LOCUS       DAOSPX010000001        38140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_1200_length_38140_cov_4.781541, whole genome shotgun sequence.
ACCESSION   DAOSPX010000001 DAOSPX010000000
VERSION     DAOSPX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1200_length_38140_cov_4.781541"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLZ75_00005"
     CDS             complement(<1..759)
                     /locus_tag="PLZ75_00005"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOS70809.1"
                     /translation="MGKNICVMLVMLSLLFTSVQGQVTEGEKKLRSQSADTAMGWKKG
                     GIIAVNVAQTSLTNWAAGGQKSMAINGIFSRFANLKGEKFTWDNSLDLGYGLLKQGDE
                     GYKKTDDKIDFLSKYGRQAFKNLYYAGLMNFKTQMQPGYKYDGEEKTKISNLFAPAYL
                     LTALGLDYKPDAYFSAFIAPLTAKFTFVTDDDLSAAGAFGVKPGEKSRGEIGGYLRAI
                     YSKSDFTSEFLKNVSFTTKLDLFSNYAHNPQNVDV"
     gene            complement(858..1193)
                     /locus_tag="PLZ75_00010"
     CDS             complement(858..1193)
                     /locus_tag="PLZ75_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOS70810.1"
                     /translation="MAKFEVYRSGKNNEFRFRLKADNGQNILSSEGYTTKAGCLNGIA
                     SVKKNSTDPKRFAKTTTPGNMFRFSLTAANNEIIGVSQNYKSESGRDNGIDSVMRNAE
                     KADIVEVDN"
     gene            1500..3038
                     /locus_tag="PLZ75_00015"
     CDS             1500..3038
                     /locus_tag="PLZ75_00015"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOS70811.1"
                     /translation="MKDKKEFIASISSGYACKGDNILLGGAVFEGEAMPESQVRIPLK
                     TLNRHGLIAGATGTGKTKTLQVMAEQLSEKGVPVLLMDIKGDLSGIAMPGKETESIAG
                     RHARIGIPYSVKGFPVELMTISKQNGVRLRATVSEFGPVLLSRILDLNDAQAGIISVI
                     FKFCDDNGLPLLDLKDLKSVIRYATSEGREEFEKEYGRISSASTGVILRKVLELEQQD
                     ADLFFGELSLEISDLLRKDKNGNAFINIVRVTDIQNRPKLFSTFMLCLLAEIYDVMPE
                     IGDREKPELVIFIEEAHLIFNRAGRTLLNEIEIIVKLIRSKGIGIFFVTQNPTDIPNS
                     VLAQLGLKIQHALRAFTAADRQAIRLAAENFPLSDYYKTDMVLTSLGTGEALVTALNE
                     KGIPTPLAVTMVRAPMSRMDILSPEEIAEINNSSELVRKYERVIDRDSAYEILDRKIV
                     SVRKSQEGRAAGKGGGSRHEGKPERTTRRAPSRRRTTIHPVVKVLTSATFIRGVMGIL
                     KKHF"
     gene            complement(3163..3585)
                     /locus_tag="PLZ75_00020"
     CDS             complement(3163..3585)
                     /locus_tag="PLZ75_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70812.1"
                     /translation="MSPNFSLGKSEIWNRTVRGVKKVVRWIIALLVMATAVFVYVNYF
                     HTFSEGYRAGTLQKFSRKGVIFKTYEGELILSGAGAENEIIISPEKFSFTMVNKDLVR
                     QFDTLQGQNVIVHFIQKNGAVFWRGESKFLVDSVRSRR"
     gene            3897..4988
                     /gene="proB"
                     /locus_tag="PLZ75_00025"
     CDS             3897..4988
                     /gene="proB"
                     /locus_tag="PLZ75_00025"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOS70813.1"
                     /translation="MNPKFNRVAIKVGSNVIAKSDGSLNTGRMLRIVEEIAILHKNNI
                     EVILITSGAVAAGRSEVVPSRKTSIISSRQIWAAIGQVKLMGTYQFLFGKYGIQAGQL
                     LATKDSFRDRMHYLNMKNCITAMLDNKVLPVVNENDAISITELMFTDNDELSGMISSM
                     MNCGSLFILSNVDGIYTGEPGSENSQLIREIDDNSGDVSQFISMSKSGFGRGGMHTKY
                     SIAMKTAAEGIDVYIANGTRDTIITDIIREKDVPCTRFIAADIKKTGVKKWLSHSGTF
                     AKGIVYINRGARDALTGDKASSLLMIGVSKVEGFFKKGDIIRIFDDANNQIGIGKSEY
                     DSEKVELYMGEKRKKPLVHYDYLLINEKN"
     gene            5005..6252
                     /locus_tag="PLZ75_00030"
     CDS             5005..6252
                     /locus_tag="PLZ75_00030"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOS70814.1"
                     /translation="MDCTPIFQSALSASRKLNNLDTDTVNRVLNHLADKTEENIPLIM
                     AENEKDLSRMDRQDPKYDRLQLTPGRIKNIAADIRNVSRLDSPLGRISYDYTKPNGLR
                     IKKISVPFGVIGIIYEARPNVTFDVFSLCLKSGNACILKGGSDAIHSNTAIVKIIKDS
                     LADFDITGDIVALLPPGREETAQLLNAVNYVDLIIPRGSQSLIDFVRKNATIPVIETG
                     AGICHSYFDEYGDRETGRKVIHNAKTRRVSVCNALDCLVIHESRLDDLGYIVGLCQDS
                     DVIIQADEKALKALRGKYPDNLLEAATDDSFGTEFLDYKMAVKTVSSFDEAIEHIAQH
                     SSQHSEAIISGDRERQEMFCKMTDAAAVYVNTSTAFTDGAEFGLGAEIGISTQKMHAR
                     GPMALEALTTYKWIIEGNGQTRP"
     gene            6278..8539
                     /locus_tag="PLZ75_00035"
     CDS             6278..8539
                     /locus_tag="PLZ75_00035"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOS70815.1"
                     /translation="MNSDSDKIKKFYEDLPGNIARIRESLNRPLTFTEKVLYNHLGDQ
                     NKVVSFIRGIDYAEFSPGRVAMQDATAQMAMLQFMMAGKLNTSVPSSIHCDHLITAFR
                     GAESDLAKSRETNREVYDFLRSVSMNYGIDFWEPGSGIIHQIILENYAFPGGMMVGTD
                     SHTPNAGGMGMIAIGVGGADAVDVMAGLPWELKFPKIIGIRLTGKLSGWVSPKDIILK
                     ITGMLSVKGGTGSVLEYFGEGAESISATGKATICNMGAETGATCSVFGFDRMMADYLI
                     ATGRQDIAEMATAAGEHLRSDPEVFDDPAKFYDNYLEINLSELEPYINGPFTPDLATP
                     ISQMKETAEKNNWPVEISAGLIGSCTNSSYEDISRAASVVKSALREKLTAKSGLRVTP
                     GSEMIRETIERDGLLAIFREIGADIYANACGPCIGQWERPGQEDNPVNTVIHSFNRNF
                     AKRNDGNPNTHAFVASPEIVTALTIAGKLTFNPLTDSLINSEGRPVNLPEPRGISFPS
                     EGFSITEGKRMEARQRKKATSVIINPRSERLQTLTPFKAWDGRDLTGLLLLIKARGKC
                     TTDHISMAGKWLRYRGHLENISNNYMIGATNAFNDKTDCILNQLNGAYEPVPAVAFSY
                     KRKGLGSIVVGEENFGEGSSREHAAMEPRFLNVRAIIVKSFARIHESNLKKQGILTLT
                     FSDKNDYAKIRETDRIDITGLRDFEPGKPFTLILHHSDGTRDTIFANHSYNESQFAWF
                     RAGSALNLIRRQQ"
     gene            8580..9854
                     /gene="icd"
                     /locus_tag="PLZ75_00040"
     CDS             8580..9854
                     /gene="icd"
                     /locus_tag="PLZ75_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOS70816.1"
                     /translation="MNGEKIRFETGKMIVPDLPLVPFITGDGTGPDIWNASVRIFDAA
                     VEKSYGGRKKIIWKEVLAGEKAFGITGNWLPRETLDAFRDHLVGIKGPLTTPVGEGMR
                     SLNVALRQELDLYTCYRPVRYFNGVPSPVKRPESVNMHIFRENTEDIYAGIEYLNGNP
                     DTEKLKSYLINEMGVRSIRFPETSSIGIKPVSVEGTERLVRQAIRFAIERKLPSVTLV
                     HKGNIMKYTEGAFMKWGYALAEREFRDKVFTWQQYSKIAGIKGEKEADDILSEAVKEG
                     KVIIKDVIADAFLQQILTRPGEYSVIATLNLNGDYISDALAAIVGGIGIAPGANINFE
                     TGHAIFEATHGTAPKYAGQDKVNPGSVILSGALMLEYLKWEEAAQCIYRNLQKTIQEK
                     KVTYDFHRLMENAQLLKTSEFADAIIKNIYSE"
     gene            9869..11227
                     /locus_tag="PLZ75_00045"
     CDS             9869..11227
                     /locus_tag="PLZ75_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOS70817.1"
                     /translation="MENNKLLSKLEKTVEESCRIDNDLFEKYDVKRGLRNSDRTGVLV
                     GLTNIGDVVGYDKENGKVIAIPGRLLYRGIDIESIAHGFQKEHRHGFDETVYLLLSGN
                     LPDRTELEEFSSMLAARRGLPDHFIKDMILSMKGRDVLNMLARSVLGLYTLDEKADDI
                     SLSNMIDQSLNLIAKFPSIIAYSYQALRHLYHGKTLSIRHPRPNLSTAENFLHLIKGD
                     GSYTRLQTDLVDLSLVLHAEHGGGNNSTFTMRVTSSTETDIYSAVTSAIGSLKGPLHG
                     GANLKVLTMMEDIKRNVSNWKDEKEVASYLLKILRKEANDKTGIIYGIGHAVYTISDP
                     RAILLKEKAAELARESGRTDEFELYALVEKLTPEVFRQFKGEKSAKVVCINVDFYSGF
                     VYSCIGIPKELYTPLFAMARIAGWCAHRIEELTFQAKRIIRPAFKNVYGTRDYIPIDA
                     RD"
     gene            complement(11317..11895)
                     /locus_tag="PLZ75_00050"
     CDS             complement(11317..11895)
                     /locus_tag="PLZ75_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOS70818.1"
                     /translation="MSLLLKGTKTEQNLLKAFAGESQATNRYEFFALVARKEGFEQIA
                     NIFQETALQEKEHAKRFFSFLEGGMVEISASYPAGRVGTTSENLKAAAEGEHEEWNDL
                     YPHFAEVAKEEGFPEIAAAFRMIAKVEAEHERRYLKLLQNISEDKVFMKDGKVWWKCI
                     NCGFVYESEKAYEKCPACLYPKSYMQLLEQNY"
     gene            12038..13387
                     /locus_tag="PLZ75_00055"
     CDS             12038..13387
                     /locus_tag="PLZ75_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HOS70819.1"
                     /translation="MKKYFWIIPALILALSACRDNQKAVTPETALGAYINSADDTYKW
                     EIREEFSSDGLQTAILKLTSQKWREIVWNHQLTVISPAGTPSDGALLFITGGSNRNGE
                     PKWHSRNNEDFKMFSMIAAKNNAVVAILSQVPNQPLYDDLTEDALISFTLHNFKNDRD
                     FTWPLLFPMTKSAIKAMDAVQEFSEQKLNHKIRGFVVSGASKRGWTTWLTGSQDKRVL
                     AIAPMVIDVLNMPVNVPYQKEAWGDYSEEIMDYVRLGIAQDLESQEGRDLATMIDPYS
                     YRASLTMPKLIIIGTNDEYWPVDAVKKYFSDLPGEKYIHYEPNTGHGLGDGVNVAKSL
                     SAFFNITINKKSHPVCEWQVENDGERTALVVRASPELIRAGLWICDSPDRDFRDNRFI
                     EKNIEYENPGSVKVTVDNPPAGFRAFYVELVYPDPVEGTYSRSTRMFVADSGELFLD"
     gene            complement(13453..14730)
                     /locus_tag="PLZ75_00060"
     CDS             complement(13453..14730)
                     /locus_tag="PLZ75_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70820.1"
                     /translation="MKKTLLLLAFLMIILQSYSQRAVFPADTLRKNAINIYMESSDFI
                     RKEIPYVNYVREIKDADVYIISTRQNTGSYGTEYTYFLVGQGKFAGMADTLTFTSAPD
                     ETSEITRYKQVNNLKMGLMRYVARTPLAGYINISFTEPLLETIYTDKWNSWVFRSSVS
                     GFLQGEKTFRSRNLSGSLTASRITENWKINLLSELNQNETKYLINDSITIRGTNSSKN
                     LDALVVKSISNHWSAGARAKLRSSSFNNENISISFMPGIEYDIYPYSESTRRQLRILY
                     TAGYTFVNYIDTTIYDRIRENLWSHSLSASYEIVQKWGNINLVAEYRNYFHDWSKNNL
                     SLTAYLDLRIARGLTVSFMGSGSMVHDQLGLVRQNMPLDQILLQRKKLATQFEYFTSF
                     GFTYTFGSIYNNVVNPRFGNSGGGMMMMPFMMP"
     gene            complement(14897..16129)
                     /locus_tag="PLZ75_00065"
     CDS             complement(14897..16129)
                     /locus_tag="PLZ75_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70821.1"
                     /translation="MDKTININLGGILFHIDEEAYRILRDYLLALSNKMRNTPGWNET
                     VEDIELRIAEIFLSKKSAASVIDSSDVDEMILIMGKPEDFTESSRAGNAFNEMFRALG
                     NIFWLVIRAVIVLAGLSFIVAGFFAAAAFIMVFVFRFPGIYAPGAAGINISYLPEFLN
                     YVINPKMVPWISFLTVITVILPLLALVYLGIKMVFRLRAADGIFFLAGFVIWILGVTI
                     LSILLFNEGISFVETARSGYREYLTGPPETLYIKQASGIAALDTDNEISIPGKNYFVF
                     IDEVNKQLYIGAHLDIMPADGKSAWIDVTKRSSGKSKTDAAKKADRIQYNYRISGDTL
                     FLDEYFTYPEAVKWSFDVVDVHVNVPRGTKISIDSGIRRMLGGNIGKDFVNDYSESTM
                     IMTRKGLEDLDSEQKTNP"
     gene            complement(16160..16486)
                     /locus_tag="PLZ75_00070"
     CDS             complement(16160..16486)
                     /locus_tag="PLZ75_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOS70822.1"
                     /translation="MDLVNTKAQMRKGILEYCILSVISRGSSYSSDIINELKKAEVIV
                     VEGTLYPLLTRLKNSGLLSYRWEESQQGPPRKYYELTAKGRNYLADLDRSWDELVESV
                     SMLRAK"
     gene            complement(16534..17124)
                     /locus_tag="PLZ75_00075"
     CDS             complement(16534..17124)
                     /locus_tag="PLZ75_00075"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOS70823.1"
                     /translation="MKITVSINLGGYAFNIDEDAYGELKRYLRELELHFAGEESSSEI
                     LSDIEARMSELFRARLSAYRQVITMEDVRYAVSVLGRPEDFSEEGSNSGKQRDSSSGY
                     HRMYRDPDRRIIGGVCAGIGAYWSLDPWIVRIIFIALILAGGLGIIIYLILYVAIPEA
                     RTTSQKIEMKGDPVNIKNITDAVRDEFDRVRKNMKL"
     gene            17361..18344
                     /locus_tag="PLZ75_00080"
     CDS             17361..18344
                     /locus_tag="PLZ75_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS70824.1"
                     /translation="MKKYKWGILAPGKMSAKFTKALKTLDNAELHAVGSRDVERAALF
                     ARDYGFRNYYGSYEELAADPELEIVYIASPHSHHYEHTMLCLRNGKHVICEKAFALNA
                     GEVAEMIDEAGKRKLFLMEALWPPFQPFYQKAKEILSSGILGRINYMNGYFSFIPPFN
                     PEDRKFNLALGGGSLLDIGIYPVIDALTFLGVPSDIKASAIFGQSGSEESLSVIFSYD
                     NRCMASIYSSFRTSAGIGSELYCENGNMTVARGRDMNQTVILTPHDREKEEFVFRPPA
                     MGYHWEAAEVMKCLDEGRTESSIVPLSFSLDLITTLDRIRIAAGIEFPGSG"
     gene            18355..19029
                     /locus_tag="PLZ75_00085"
     CDS             18355..19029
                     /locus_tag="PLZ75_00085"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOS70825.1"
                     /translation="MSGENHSSLSEEAFLKKKFLLSLIIPGIFVFLMVLAKVIELLFE
                     TDLSVLGIYPLSFRGLPGIILSPFIHADFRHLLNNSLPLFLLSVSLFYFYSEIALKVF
                     IMTWIFTGLLVWTGGREAWHIGASGIVYGLAAFLFFSGILRKYYRLVALSLLLVFLYG
                     EMVWGLFPGIYKNISWESHMLGFISGIFLAIIYRNEGPQKPVYDWMEEEDEDEEEEEG
                     GKRTEE"
     gene            19107..20492
                     /locus_tag="PLZ75_00090"
     CDS             19107..20492
                     /locus_tag="PLZ75_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70826.1"
                     /translation="MISIEKKLNPADLSDKLAGFWEISGRKINLIEKYYDESQGSPVF
                     TVKGRYSTRGWTEWTQGFQYGSAFLQFDATGDKKMLDYAREKTVRKMAPHVSHTGVHD
                     HGFNNVSTYGNLLRLMNEGKIPFSEWEKNFCELALKVSGAVQASRWTAIRDGGYIYSF
                     NGAHSLFVDTIRSCRILMIAHRLGHVLQAEGDIRISLLERALEHLKSTARYSVYYGEG
                     RDIWDTEPGRTAHESVFNPADGSFRCPSSQQGYTGFTTWTRGLAWAVCGFSEQLEWID
                     FLKREGTDHPADLNNIEKIIDRAARVTCDFYIKNSPSDGVPYWDTGAPGLVYLKDYLN
                     KPSDPFNEYEPVDSSAAAIAAQGLLRLGHFLKQKGDVYSGSRYWQAGLKVLDSLLSEP
                     YLSTGEEHQGLLLHSVYHRPNGWDHIPEGSKIPNGESSMWGDYHIREACLYLHRIISN
                     ETYYNFFSYTG"
     gene            20489..21322
                     /locus_tag="PLZ75_00095"
     CDS             20489..21322
                     /locus_tag="PLZ75_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS70827.1"
                     /translation="MNLQPITDLQRLCIHTITVKPWNIGEIAANFSSAGVKGITVWRD
                     TLAGRDIRQTGRMLRDHGLSIVSLCRGGFFPSADRARRRAAIDDNRKAIEEAHELGTD
                     LIVLVCGADPSQSLEDSRKQIHDGIASVLPDAESAGVRLAIEPLHPMFADTRSAINTL
                     AQANDLAEILESPSAGVAVDVYHLWWDPDLEKEIKRSGKNGKLFAYHVSDWKSPTVDM
                     LNDRGLMGEGCIPLKKIRSWVEDSGFSGFIEVEIFSDIYWSRDQHSFLQDIVSSYKKY
                     V"
     gene            21416..22567
                     /locus_tag="PLZ75_00100"
     CDS             21416..22567
                     /locus_tag="PLZ75_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS70828.1"
                     /translation="MKQHTVGIIMNGVTGRMGTNQHLLRSIAEIIRQGGVKCGSSETI
                     MPDPILVGRNETKLKNLCNISGISKMSTDLDEVLKDRNYQLYFDAQTTGRRVDAVKKA
                     IRAGKHIYCEKPVAISTEAALELYELCRDAGLKNGVVQDKLWLPGILKLKRLIETGFF
                     GRILSVRGEFGYWVFEGETIPAQRPSWNYRSEDDGGIILDMLCHWRYVLDNVFGKVKA
                     VSCLGATHIPERFDEEGKAYKCTADDAAYATFELENGIIAHFNSSWAVRVRRDDLLTL
                     QVDGTRGSAVAGLRECYIQHYGNTPKPVWNPDIPQPIDFFEGWSRVPEQENYDNAFKA
                     QWELFLKHVVKDEPFPWDLHEGAKGVQLAEKGMESWKKRCWTEIPELKK"
     gene            22654..23433
                     /locus_tag="PLZ75_00105"
     CDS             22654..23433
                     /locus_tag="PLZ75_00105"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOS70829.1"
                     /translation="MNNKPVAVITGASRGIGRAIAISLASEKFDIAAIARTVESEGME
                     SLAREITAKNVAFFPVGLDIACTDCQDEVVKNILGRYGRIDLLVNNAGVAPLKRNDLL
                     EMTEESYERVMNINLKGPVFFAQRIAREMIYLGRQISDYRPAIIFISSISSTRTSVNR
                     AEYCISKAGLSMASSVFADRLAADNIRVFEIRPGIINTDMTARVKDTYDKLIADGMVP
                     QKRWGYPEDIGRAVASLARGDWDFSTGAIFDISGGLNITRL"
     gene            23573..24100
                     /locus_tag="PLZ75_00110"
     CDS             23573..24100
                     /locus_tag="PLZ75_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOS70830.1"
                     /translation="MNTPTNAEEKMNADVRKTTLNWHGEKVLGHHTGTILLRDGWIIF
                     DNNRITSGEFTIDMTTIKDDDSNERLEGHLKSDDFFGVDKHPLSKIVILESEAFDKGK
                     GLVKGHLTIKGITHPVEFSATMQKNNEGTSFFALIIVDRSKYNVRYGSGSFFENLGDK
                     AIYDEFRVRVNLHLN"
     gene            complement(24114..24812)
                     /locus_tag="PLZ75_00115"
     CDS             complement(24114..24812)
                     /locus_tag="PLZ75_00115"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOS70831.1"
                     /translation="MKLKHVFKTVFRRVSARIRSGPLKGKKWSIASGSRFIRGDYESY
                     KTRAFLDNFEPGSIFLDIGAHFGYYSSMAATVSRGEGWIYSLEPRPMNIKFFRRHIEI
                     NDFRNITLIEAAAGRYDGFVRFDNRHGSATGFVTGDGNLKVEQVSVGGLIKRGVIRPP
                     DFIKIDVEGGEMEVLADLEEFISREHPKMLVATHGKECRDFTENFLRKNKYSFRVLNP
                     EAVSGDTEIIAIPV"
     gene            24947..26221
                     /gene="fucP"
                     /locus_tag="PLZ75_00120"
     CDS             24947..26221
                     /gene="fucP"
                     /locus_tag="PLZ75_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HOS70832.1"
                     /translation="MANSVSLKNDRQKLVTEGYLIPFMLVTSLFFLWGFAHGCLDVLN
                     KHFQELLSISKAKSALVQFVFYGGYFLMAIPAGLLMQRFGYKKGIIWGLLLFCAGAFL
                     MFPATFIQTFGSFLACLFIIACGLTFLETAANPYTTVLGPPESAERRINFSQSFNGLG
                     WIAGPLIGGLLIFSNTESENKFSSIALPYMVIGTLVLLVALLFWRVTLPEVKEESQKA
                     DDNGPDSNAADYDVSWRNLFRHRHFVLAVIAQFFYVAAQTGVNSFFINYVTEEMPAIS
                     NQAASRILAFGGMGLFWLGRFTGSTIFMRLAKPNRLLAIYALMNVITMIIVMAGLGWF
                     SVVALFSTYFFMSIMFPTIFALGIKDLGPLTKKASSFLVMAIVGGALIPMLMGHIADI
                     STMATGFAVPLACFAFILYFGLDGYKVRIAGK"
     gene            26275..27456
                     /locus_tag="PLZ75_00125"
     CDS             26275..27456
                     /locus_tag="PLZ75_00125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS70833.1"
                     /translation="MTGTPKKNLRISFVTSGHFPDDDRIFYHQGRTLAEKGFPVQIVS
                     SKTDTDCSAEGMILNCFDGGSMTKREKIRKFTETLRQFNPDVLICSEPLTVLAGRLFR
                     HGSKKRIRIIYDITEWYPSKKNLAGKRAFCKYITFIKLLFFNILSSALADAFIFGEYY
                     KSRPYRFLFPFRPFIFSTYYPDLKYIEFKKPEPADGKLRLSYSGKISREKGFVNFISV
                     IDGLSAVHHDLEIEVKITGWFETDDDRKECETYLKNVNADIAFIMKGKQDFAGYTGSL
                     HETDIFLDLRSDDPENKHCLPIKLFYYAAAGRPVIFSDLKAIRKEVEVEKFGFLVKPG
                     DTEMIVRLISEYLENSDLYCRHCNEARNLAETKYNWGRISSEFVKFVINAASRTGAAD
                     K"
     gene            27464..28672
                     /locus_tag="PLZ75_00130"
     CDS             27464..28672
                     /locus_tag="PLZ75_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70834.1"
                     /translation="MLSRNTSITIVTKFLILAANFALVVASTRLWGSEGRGEIALLLA
                     NISIIAIFGNVFCGSTIAFHTPVMNRDILLTAGSAGALLVSLTGSLFFSLLFGFRNFT
                     FLLPMSFFLSLSTLVTSYRLGKKDFVRYNLLTLLNPLSILIFLYILYALLHKTGLETY
                     YHAWYIGTGSVSLFALTSLLRRNPYKRPGLKTADLKRILNYGFKNEFNYLLQFLSYRL
                     SYYFIARILGYVQLGIFSVVISVSEAVWIISRSMSAVHYSNVINSNDFIRSRKETVAF
                     ARQSFIISIAVLAVSVLVPVKVYQLVFGAEFGIVRKYILYMIPGIMAISVSNLYGHYF
                     AGTGKLNIIRNKSLIGLLAIIISMPFLIKAFQMEGVCIAVNISYILSSVYLWVYFRRE
                     GKSLNFKTQA"
     gene            28679..31822
                     /locus_tag="PLZ75_00135"
     CDS             28679..31822
                     /locus_tag="PLZ75_00135"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HOS70835.1"
                     /translation="MKKHLFIFAVFLISGFTNFSEACTTFIISGRYTPDSRPILYKHR
                     DTGVTDNALAVFSDGKYNYVGLLNSDKNWNNEVWGGFNSAGFAIMNSVAYNKNIGDTT
                     GLADQEGRIMKLALQNCATVDDFEKLLAELPKPLGVDTNFGVIDALGGAAYFETGNYG
                     FEKIDANDAAAAPYGYLIRTNHAFTGPVDKGFGYIRYSTANEALYRAAAMNRYDPQYL
                     ISNISRNLYHSLTDVNLRDVLPADSEREKFVFFEDFIPRYSSASAICVAGTKAGEDPS
                     GTVMWTLCGFPLTTAVVPVWLTKDKILPAAVSMKDDLHSPLCDAALRLKDRCFPVKRG
                     SGTKYLNLAALLNGRNTGILQLLESFENEIFKKADELIRSAPGMIPDEKGIGRFYSWL
                     DDYIEDSYRMLSETGTAGGQKLPPELVDPPREFSVMPFWFWNDTLKDDEIIRQIADFE
                     SHGVYGFVIHPRVGLPQNVKWLGPEMIHAMNIAISEAARRNMYVILYDEGMYPSGSSS
                     GQVVEKNPEHAARGLAKIDLKEGEELRLEEGWKLVTIAQRPGNRRTAIIERPSGGVIR
                     GLHYLNEGEERLLEHSPPAGDLLNPDAVSSFISLVYDKYAREFGKYFGNTIMGIFTDE
                     PSPLGRDALRGMVPGNASLLPRIKKILGYDITPHLADLWYNDYPDSERHRTDYYHAIN
                     ICLEENYYRRLGNWCFLHNISLMGHPAGSMDIGTQRYFQIPGQDLVWRYVEPGPKALE
                     GQHSTMAKGASSAMIHNGYRRNSNELYGAYGHELTWEEMLWLAGWCFVRGHNLLIPHA
                     FFYSVRGPRIDERPPDVGPNASWWPDYKPYADACRRLSWLNTDSRHICDLAILCEATW
                     LPDRPAKVLYRNQRDFNYLEIRHLWEDAKIDSRGIHIGDMTYRALIVDSLSFIPPRVI
                     PKLKKLARHRHLILRSDSKLASVCNGALVYGSGDELRAAVNKITPPDIVLSPSSGNIR
                     CRHVEKDGDHYFMLFNEENSEVTANISLKIENDIKNTGNTREWIDPFTAKTSTAGTDR
                     AVYFKPFEMKLLRIARK"
     gene            31963..34026
                     /locus_tag="PLZ75_00140"
     CDS             31963..34026
                     /locus_tag="PLZ75_00140"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS70836.1"
                     /translation="MVEFIRDPEGVRIMDLRPEFTWIVPLHAKNQTACEILVSSGREK
                     LEKDIADIWNSGKMIAPASCEVEYGGPLLSPDSRYFWKVRIWDEKDRPAGYSEIRSFT
                     TGNPAGYATTANKFRSSSIHPLKLTRISEGHYFADFGKDAFGKLIITGISPDIADTLT
                     VHLGEKLAAANTVDRNPGGTIRYYRVKIPLKPGQNSFTAELPPDERNTGPSAVHLPDS
                     LGIIAPFRYAEIENCSFRLEKNNLVQEVTSYFFDDGAGSFTCSDTILNQVWDMCKYTM
                     KATSFAGIYIDGDRERIPYEADAYINQLGHYYTDREYSMGRLTNEYFIKHPTWPTEWI
                     LHTVLMFWNDYMFTGNPESLREYYEELRHKTLISLAREDGLISSKNATDRIMEDIGFS
                     DPGERIRDIVDWPPAQKDTGWKLAGPEGERDGYDMVEINTVVNSFYYRNLFLMSEIAG
                     ILGKTDDSAFFRRESEKVRKSINEKLLDRSKGIYLDGENSEHSSLHANMMPLAFGIIP
                     AEYRKSVIEFIKSRGMACSVYGAQYLLEGLFREGEAEYAVSLMNATHDRSWWNMIRTG
                     STMAMEAWDMKYKPNSDWNHAWGAVPANIIPGYMWGIAPAEPGFAAVTIRPQLSGLTY
                     SKIEVPTIRGKIVAEFRAKGKSSVYTVNIPANMKGRLILPDREAFELSPGINSLKQVH
                     TVKTQ"
     gene            34058..35704
                     /locus_tag="PLZ75_00145"
     CDS             34058..35704
                     /locus_tag="PLZ75_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS70837.1"
                     /translation="MKTAIFPLLFTFLLVAACPSCSNTDIYRADIIVYGGTSAAVTTA
                     VQAARMGKSVIVVSPDRHLGGLSSSGLGYTDTGNKEVIGGIAREFYQYVYQHYERPES
                     WKWQEKSSYGNRGQGIPAIDGEKRTMWIFEPHVAEAIFEKFTEDHNITVFRDEWLDRE
                     ENTIKKDGRIISFRTLSGKTFRGKIFVDASYEGDLMAAAGVSYHVGREANSVYDETWN
                     GVQKGLYHHKHYFRDNISPYKIPGDPSGGLLPRVSAGAPGENGSADNKIQAYCYRLCL
                     TKHPENKIPFTRPEGYDSTQYELLVRVLAAGWDEYFEKFDEIPNLKTDVNNHGPFSSD
                     NIGMNYDYPEASYERRREILNEHITYQKGLLYFTATDKRIPEWLRNEINLWGYAADEF
                     TDNGNWPYNIYVREARRMTGEYVMTEHDVLVRRPVPEPVGMGSYTMDSHHVQRYVTPE
                     GYVQNEGDIGVPAPEPYQIAMGSLLPKRSECSNLIVPVCVSSSHVAFGSVRMEPVFMI
                     LGQSAGTIASMAIDKNKNIHDLSYSGIREKLLSDGQILEY"
     gene            35963..36610
                     /locus_tag="PLZ75_00150"
     CDS             35963..36610
                     /locus_tag="PLZ75_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70838.1"
                     /translation="MKKIYLCIAAAFILAASPSLAQFEKGSFITGVSSTFGVGSFGTD
                     LMSIGTTSQKIKYNEEEVKGTLKGGKYFNLSLLPRAGYFVIDNLSVGLDLILGYNSWK
                     YPEEGKNGVEKTTSTSVAAGPFVRYYYPLEKLYPFVQANVAFGIDKTKGYYSSTNLLS
                     VYGIGVGAAKTIGEKVMVDAVLGWNSASLTDEDNDKEIYGSFGVRVGFSYFFSIK"
     gene            36650..37297
                     /locus_tag="PLZ75_00155"
     CDS             36650..37297
                     /locus_tag="PLZ75_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70839.1"
                     /translation="MKKIYLFVAAICVLSVSHTFAQFEKGRILAGISSGLQVTDPGAS
                     LMNLGLGRIKYKEDGERAEKGPKEFNINLLPRAAYFVMDNLAVGLDLQVYLNVLKDRT
                     TKGSNYVNTFLAAGPFVRYYYPLEKIHPFAEANMGLGISRTLDSSDGENGFLYGVGIG
                     AALPLGENLMLDGQVGFSSNIWKDVDPDKKQASTKYIYNSFGGRVGFILLFGKGK"
     gene            37541..38035
                     /locus_tag="PLZ75_00160"
     CDS             37541..38035
                     /locus_tag="PLZ75_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70840.1"
                     /translation="MKITSILMSLVLLISQSYSLSAGTIPVELKSLEESVFIPDQEAI
                     DLAFGELNELDEYLELNTGLSYTDLQAAGSILIKDISDMVAPFGQSEEGDDEDPLGIP
                     AFWWGCFMGIIGVLLVYIFTDNNKDQTKKAFKGFLVTLVSSFAASAIIMIVILATTPV
                     VLSD"
BASE COUNT        10908 a         8565 c         8808 g         9859 t
ORIGIN      
        1 gacatccaca ttctggggat tatgcgcgta atttgagaag aggtccagct ttgtggtaaa
       61 ggaaacattt ttcagaaact cactggtaaa atcagatttt gaataaatag ctctcagata
      121 tcctcctatt tcaccccttg atttttctcc cggttttact ccgaaagctc ccgctgctga
      181 caggtcatca tctgtgacga aggtgaattt tgcggtaagg ggtgcaataa atgcactgaa
      241 atatgcatcg ggcttataat cgagtccgag ggcggtgaga agataggccg gtgcaaagag
      301 gtttgaaatt tttgtttttt cttcaccgtc atacttatag ccaggctgca tctgtgtttt
      361 gaaattcatc aggccggcat aatatagatt cttaaatgcc tgacgcccgt attttgaaag
      421 aaaatctatt ttgtcatcag tctttttgta tccctcatca ccttgcttga gaaggccgta
      481 gcccagatcc agggaattgt cccaggtaaa tttttcacct ttcagatttg caaaacggct
      541 gaatatcccg tttattgcca tcgatttctg cccgccggct gcccagtttg taagcgaagt
      601 ctgagctaca tttacggcaa ttatcccgcc ttttttccag cccatggcag tatcggcgga
      661 ttgtgaacga agcttctttt cgccctcggt gacctgcccc tgaacagagg taaacagaag
      721 agagagcata acaagcatta cacagatatt ttttcccata ttcatgataa atatattaaa
      781 tgctactgat ataccggata taaactgaac cggcctttga aggccggttc agtatttctt
      841 tgaagcaaag aggattttta attatccact tccacgatat cggctttttc tgcatttctc
      901 atcacagaat caattccgtt atcgcggccg ctttcacttt tataattctg acttactcct
      961 attatctcat tatttgccgc tgtaagggaa aaacgaaaca tgttccccgg agtggttgtt
     1021 ttagcaaatc ttttcggatc tgttgaattc ttctttaccg atgcaatacc attcaggcat
     1081 ccggcttttg ttgtgtatcc ttcactgctg aggatgttct ggccgttgtc agccttcaga
     1141 cggaaacgga attcattatt ctttccgctg cgataaactt caaattttgc cattttaata
     1201 gatttggatt tataaattag ttgttcaatt taatattccc tcctattact gtttttaagt
     1261 caataagttg tgaggccgca aagataaaaa cattttttta attaacccga atagaaataa
     1321 ttttccctcc tgttctgcaa gctgtaagac acgccggtca ggcatatccg gaaaaatgct
     1381 gtttatccct gaaatatctt cagcagattt ctgtggcagg aaataatccc ttaaatttca
     1441 gctttcattc agaccggtaa ttttcaattc atatcagcaa tattcagaaa aggaataata
     1501 tgaaagacaa aaaagaattt atagccagca tcagttcagg ttatgcatgc aaaggtgaca
     1561 acatcctgct tggaggagcc gtttttgagg gggaagcaat gccggaatca caggtaagaa
     1621 ttccactgaa aacattgaac aggcatggtc ttattgcggg agccaccggt accggaaaga
     1681 ccaaaaccct tcaggtaatg gctgaacaat tgtcagagaa aggagttccc gtgttactga
     1741 tggatatcaa gggcgacctg agcggaatag caatgccggg aaaagaaaca gaatcaattg
     1801 ccggccggca tgccagaatc ggaattccct attccgtaaa agggtttccg gttgaactga
     1861 tgaccatatc aaaacaaaac ggtgtaaggc ttcgtgcaac cgtatccgaa tttggtccgg
     1921 tgcttctttc aagaatactc gatctgaacg atgcccaggc aggaattatt tcggtcatat
     1981 tcaaattttg tgatgacaac ggacttcctc ttctcgacct gaaggatctg aaaagtgtaa
     2041 tccgctatgc cacatcggaa ggcagggagg agtttgaaaa ggaatatggc aggatatcat
     2101 ccgcctcaac cggcgtgata ctgcgaaaag tactggaact cgaacagcag gatgcggatc
     2161 tgttttttgg cgaactttcc cttgagatca gcgatctgct cagaaaggac aaaaacggca
     2221 atgccttcat aaacattgta agggtgacgg atatacagaa caggcctaaa ctgttttcaa
     2281 cctttatgtt atgcctgctt gccgagatat atgatgtgat gcctgaaatc ggagatcggg
     2341 aaaaaccaga gctggtaata tttatcgaag aagcccatct catattcaac cgtgcaggca
     2401 ggacccttct gaacgaaatc gaaatcattg tcaagctgat acgttccaaa ggcattggga
     2461 tcttctttgt gacacagaac cccacggata ttccgaattc agtcctggca cagctcggcc
     2521 tgaagattca gcacgcacta agggcattta ccgcagctga caggcaggca atcaggcttg
     2581 cagcagaaaa ttttcccctg tcagactatt acaaaactga tatggtcctt acaagcctgg
     2641 gaacgggcga ggctcttgtc acagccctca atgaaaaggg gatacccaca ccacttgcag
     2701 tgaccatggt cagggccccg atgagcagaa tggatattct ttcccctgag gagattgcag
     2761 agatcaataa ttcatcggaa ctggtccgta aatatgagcg ggttattgac agggacagcg
     2821 cctatgaaat acttgacaga aagattgtct ccgtacggaa aagccaggaa ggccgggctg
     2881 ccgggaaggg aggaggcagc aggcatgaag gaaagccgga aagaaccaca cgacgggcac
     2941 cttcgcggag aaggaccaca atccatccgg tggtaaaggt cctgaccagc gcaacattta
     3001 tccgcggagt gatgggaata ctcaaaaagc atttctgatt aattgatgcc tgaaaagctg
     3061 attattaatg cttccggacc tgaggaatca atttttccgg cttatgatgc cagaaggtaa
     3121 attcccccgc cattgaggaa agcgcttagg tcatgaacgg aattatcttc ttgagcgcac
     3181 agaatcaacc aggaacttcg attcgcccct ccagaacacg gctccgttct tttgaatgaa
     3241 atgcacaatc acattctggc cctgaagggt gtcaaactgt ctgacaaggt ctttattaac
     3301 cattgtaaac gagaattttt cgggcgatat tattatttca ttttccgcac cagccccgct
     3361 tagtatcagt tctccttcat aagtcttgaa aatgacgccc tttcttgaaa atttctgcaa
     3421 tgttcccgcc ctgtagcctt cactgaaggt gtgaaaataa tttacatata caaaaacagc
     3481 tgttgccatg acaagcaacg caatgatcca tctgacgact ttttttacgc ctctgacggt
     3541 cctgttccag atttctgatt tgccaaggga aaaattcggg ctcatattta ttatgtctta
     3601 atgtttaccg gtcaagctgt ttcatgcaaa gtaaatcaaa attttcctta aggtcggcct
     3661 gatattgcac aagctgcaga tctgcggatt cccgcgtaca tgaaatacag gcagcaagca
     3721 tctggggtat taatgctttt ttcttcatac tgatgaagtt aattccggga taattttgat
     3781 ttcctgttat aaaaatgctt aaaaattata tatatcctgt aaaaaactac ataattactg
     3841 ttatttttgc aacattttgc tcaaatcaag gccccgccaa taaagaattg aaaaatatta
     3901 atccgaaatt caacagagtt gctataaaag taggaagcaa tgttatagca aagtcagacg
     3961 gatctctgaa taccggcagg atgttgcgta ttgttgaaga gatagcaatc cttcacaaga
     4021 acaacattga agtaatattg attacttcgg gggcggttgc agccggacgc agtgaagtag
     4081 taccatccag gaaaaccagc ataatctcgt cgcgtcagat ctgggctgca ataggccagg
     4141 tgaagcttat gggaacctat cagttcctat ttgggaaata cgggattcag gcaggacagc
     4201 ttcttgcaac aaaagacagt ttcagggaca ggatgcatta tctgaacatg aagaactgta
     4261 tcaccgctat gctggacaat aaagtgcttc ccgtggtaaa cgagaatgat gccatctcca
     4321 ttacagagct gatgttcacc gataacgatg aattgtcagg catgatttcc tcaatgatga
     4381 attgcggttc cctgtttatt ctgtcaaatg ttgacggaat ttataccggt gaacccggat
     4441 ctgaaaattc acagcttatc agggagatag atgacaattc cggtgatgtg agccagttca
     4501 tcagtatgtc aaaatcggga ttcggacgcg ggggtatgca tacaaaatat tccattgcca
     4561 tgaaaacagc ggcggaagga atcgatgtat atattgcaaa cggaacacgt gataccatta
     4621 taaccgatat catccgtgaa aaagatgttc cgtgcacaag attcattgca gccgatataa
     4681 aaaagacggg agtgaaaaaa tggctttccc actccggaac atttgcaaag ggaattgttt
     4741 acattaacag gggagcccgg gatgccctga caggggataa ggcctccagc ctgcttatga
     4801 taggagtatc aaaagttgag ggctttttta aaaaaggaga tatcatcagg atatttgatg
     4861 atgccaataa tcagatcgga atcggcaaat cggaatatga ttccgaaaag gttgagcttt
     4921 acatgggtga aaagaggaaa aaacctctgg tacactatga ttatttactg ataaatgaga
     4981 aaaactgaaa attaatctcc ggaaatggac tgtacgccga tatttcaatc agcactttcg
     5041 gcaagccgga agctgaataa ccttgatacc gataccgtca acagggtatt gaatcatctt
     5101 gctgacaaga cagaggagaa catccctctg ataatggcag aaaatgaaaa ggacctttcc
     5161 cgtatggaca ggcaggatcc gaaatatgac cgcctgcagc ttacccccgg cagaataaaa
     5221 aatattgctg cagatatccg gaatgtgtcc cgcctggatt ctccccttgg caggatcagt
     5281 tacgactaca caaaaccaaa cggactcagg ataaaaaaaa taagcgtccc gttcggagtt
     5341 atcggaataa tatatgaagc ccggccaaat gtcacttttg acgttttctc cctctgcctg
     5401 aaatcaggaa atgcctgcat actcaaggga ggaagcgatg ccattcattc aaacacagcc
     5461 attgtaaaaa taataaagga ttccctggct gattttgata tcacgggcga catcgtagcc
     5521 cttctgcctc ccggcaggga agaaaccgca cagcttctga acgccgttaa ttacgttgac
     5581 cttatcattc cgcgcggaag ccagagcctt atagatttcg taaggaagaa tgccaccata
     5641 cccgttattg aaacaggtgc aggaatatgc cacagctatt ttgatgaata tggcgaccgc
     5701 gaaacaggaa ggaaagtaat ccacaatgcc aaaacaaggc gtgtaagtgt ttgtaacgca
     5761 cttgactgcc tggtaataca tgaaagccgg ctggacgacc tcggatatat tgtaggtctc
     5821 tgtcaggatt cagatgtgat aatacaggct gatgaaaagg ccctgaaggc cctcagggga
     5881 aagtatcccg acaatctgct tgaagctgcc actgacgatt ccttcggcac tgagttcctg
     5941 gattacaaga tggctgttaa gactgtttcc tcttttgacg aagccattga acacattgca
     6001 caacacagtt cacagcatag tgaagccatt atatccggcg acagggaaag acaggaaatg
     6061 ttttgtaaaa tgacagatgc agcagctgta tatgtcaata catcaacggc atttaccgat
     6121 ggtgccgaat tcggccttgg cgccgaaatc ggaatatcca cgcagaaaat gcatgccaga
     6181 gggccaatgg cccttgaggc tcttaccacc tataaatgga taattgaggg taacgggcag
     6241 accaggccct gaacggaaca atctaaagct taccattatg aattctgatt ctgataagat
     6301 aaagaaattc tatgaagacc ttcctggaaa cattgccagg ataagggaga gtctcaacag
     6361 gccgctgacc tttaccgaaa aggtgctgta caatcatctc ggggatcaga acaaggttgt
     6421 ttccttcatc agaggaattg attacgcgga attttcaccc ggcagggttg caatgcagga
     6481 tgcaacggct cagatggcaa tgctgcagtt catgatggcc ggaaagctga acacttctgt
     6541 tccctcatca attcactgtg atcaccttat cacagcattc aggggagctg aaagcgacct
     6601 tgctaaatcc cgtgaaacca accgtgaagt atatgatttc ctccggtcag taagcatgaa
     6661 ctacggaatt gatttctggg aacccggatc cggtatcatt caccagatca ttctggaaaa
     6721 ctatgccttt ccgggaggga tgatggtggg cactgattcg cacacaccaa acgcaggggg
     6781 aatgggaatg atagcaatcg gtgtgggcgg cgctgacgcg gtggatgtaa tggcggggct
     6841 tccctgggaa ctgaagtttc cgaaaataat cggtatccgt cttacaggaa aactgagcgg
     6901 atgggtctct cccaaggaca tcatcctcaa aataacagga atgttatcag taaagggagg
     6961 taccggatca gttctggaat atttcgggga aggagccgaa tccatttcag caaccggcaa
     7021 ggccaccatc tgcaacatgg gcgctgaaac cggcgcaacc tgttctgtct tcggatttga
     7081 ccggatgatg gctgattatc ttatcgccac gggcaggcag gatatcgcag aaatggcaac
     7141 agccgccgga gaacatctca gaagtgatcc ggaagtattt gatgacccgg cgaaatttta
     7201 cgacaattac cttgagataa acctttcgga actggagccc tatatcaacg gtccgttcac
     7261 acccgatctg gccactccca tctcacagat gaaagaaact gcggaaaaaa acaactggcc
     7321 ggtggagata tctgcaggac tgattggatc atgtaccaat tcgtcctatg aagatatttc
     7381 aagggctgct tcagtggtaa aaagtgcact cagggaaaaa ctgacggcaa aatccggtct
     7441 cagggttacg cccggatcag aaatgatacg cgaaactatt gaaagagacg gccttctggc
     7501 aattttcaga gagatcggag ctgatatcta tgccaatgcc tgcggaccct gtatcggaca
     7561 atgggaaagg cccggacagg aagacaatcc ggtaaatact gtgatccatt cgtttaaccg
     7621 caattttgca aaaaggaacg acggtaatcc aaatacacat gcctttgttg catctcctga
     7681 aatagtaaca gccctgacca tagcggggaa actgacattc aaccctctta cggattcact
     7741 aatcaattcg gagggaagac cggttaatct tccggaacca cggggtattt cctttccctc
     7801 agagggattc agtattactg agggaaaaag gatggaggcc cggcaaagga aaaaagcaac
     7861 atccgtcatc ataaatccgc gctcggaaag actgcagacg cttactccct tcaaagcctg
     7921 ggacggcagg gatctgacag gtctcctcct gcttataaaa gccaggggga aatgcaccac
     7981 cgaccatata tcaatggcgg gtaaatggct cagataccgc ggtcacctgg aaaatatatc
     8041 aaacaattac atgataggcg ccacaaatgc ctttaatgac aaaaccgact gcatactgaa
     8101 ccagctcaac ggtgcatatg aaccggtacc ggcagttgcc ttttcctata agagaaaggg
     8161 actgggctca attgttgtgg gtgaggaaaa tttcggggaa ggatcatcgc gcgagcatgc
     8221 ggcaatggaa ccccgctttc tgaatgttag agccatcatt gtaaaatcat ttgcaaggat
     8281 acatgaatca aacctcaaga aacagggaat ccttacactt acattttccg acaaaaacga
     8341 ctatgccaaa atcagggaga ctgacaggat agatataaca ggattaaggg attttgagcc
     8401 cggcaagcct tttacgctta tcctgcacca tagtgacgga acgcgggata ccatatttgc
     8461 aaaccattca tacaatgaat cccagtttgc atggttcagg gcaggaagcg ccctcaatct
     8521 gataaggcgg caacagtgaa tcaataacgg aaaattgaat tattaattta aaatagagga
     8581 tgaacgggga gaaaataaga tttgaaaccg ggaagatgat tgtcccggat ttgcctctgg
     8641 ttcctttcat tacaggggac ggaacgggtc cggatatctg gaatgcttcc gtaagaatat
     8701 ttgatgccgc tgtggagaaa tcctacgggg gaaggaaaaa gataatctgg aaggaagtgc
     8761 ttgcagggga aaaggcattc ggaatcaccg gaaactggct gccccgggaa acacttgatg
     8821 cgttcaggga tcatcttgtc gggataaagg gacctctcac aacaccggtg ggggaaggaa
     8881 tgagatccct taatgttgcc ctcaggcagg aactggatct ctatacctgc taccgccccg
     8941 taaggtattt taatggcgtg cccagccctg taaaaaggcc ggaatccgta aacatgcata
     9001 ttttcaggga gaacactgag gatatctatg ccggaataga atacctgaac ggtaatccgg
     9061 atacggagaa actgaaaagt tatctgatta atgaaatggg agtccggagt atccgttttc
     9121 cggaaacatc ctcaatcggc atcaagccgg tatcggtgga aggaactgaa agacttgtaa
     9181 ggcaggcgat caggttcgca attgaaagga agctgccgtc agtgaccctg gtacacaagg
     9241 gcaacataat gaaatatacc gaaggggcat ttatgaaatg gggatatgcc ctggcggaaa
     9301 gggaattcag ggataaagtc tttacatggc agcagtacag caagattgca ggaataaagg
     9361 gggaaaagga agcggatgat attttgtcag aagctgtaaa ggaagggaag gtaataataa
     9421 aggatgttat tgccgatgca tttcttcagc agatacttac acggcccggg gaatattcgg
     9481 ttattgcaac ccttaacctt aacggggatt atatttccga tgcccttgca gccatagtcg
     9541 gcgggattgg cattgcaccc ggtgccaaca taaattttga aaccggacac gcaatatttg
     9601 aagcaactca cggtactgcc ccgaaatatg cgggacagga caaggtgaat cccggttctg
     9661 tcatcctctc gggtgctctg atgctggaat atctgaaatg ggaagaggcc gcacaatgca
     9721 tttacaggaa tcttcagaaa accattcagg aaaagaaagt tacctatgat ttccacaggc
     9781 tgatggagaa tgcacaattg ctcaaaacat cggagttcgc tgacgcgatt attaaaaata
     9841 tttattctga ataaacacgt taattatcat ggaaaacaat aaactccttt caaaactgga
     9901 aaagacagta gaggaatcat gccggattga taatgatctt tttgaaaaat atgatgtcaa
     9961 acggggattg agaaacagcg accgcaccgg agtacttgtg ggactgacga atataggcga
    10021 cgtggtcggt tatgacaagg aaaacggcaa ggtgattgct attccgggaa gattattgta
    10081 ccgtggaata gatattgaat caatagctca cggattccag aaggagcaca ggcacggatt
    10141 tgatgaaacc gtttatctgt tattgtcggg caacctgcct gacaggaccg aactggaaga
    10201 attttcgtca atgcttgcag ccaggagagg cctgccggac catttcatca aggacatgat
    10261 cctttcaatg aagggcaggg acgtgcttaa catgctggca agatctgtgc tgggcctgta
    10321 tacccttgac gaaaaagcgg atgacatttc tctcagtaac atgatagatc agtcactcaa
    10381 cctgattgca aaatttccct caataattgc ctactcctac caggccttac ggcatttgta
    10441 tcatggcaag acattgtcca tcaggcatcc gagaccaaac ctctcaacag ccgaaaattt
    10501 ccttcacctc ataaagggag atggttccta tacgaggctc cagactgatc tcgtggacct
    10561 gtccctggtt ctgcatgcgg aacacggggg aggaaacaat tccaccttta caatgagagt
    10621 aaccagttca accgaaacgg atatctattc agccgtcaca tcggctatcg gctccctgaa
    10681 aggtcccctg cacggaggag caaacctgaa ggttctgacc atgatggaag atattaaaag
    10741 aaatgtcagc aactggaagg atgaaaaaga ggttgcatcc taccttctga agatattacg
    10801 taaagaagca aatgacaaaa ccggtattat ctacggtatc ggccatgcag tctatacaat
    10861 atccgatccc agggcaatcc tcctcaagga gaaggctgcg gagctcgcaa gggaatcagg
    10921 aagaaccgat gaattcgaac tgtatgccct tgttgagaaa ctgactcccg aggtgttcag
    10981 acagttcaag ggcgagaaat ctgccaaagt ggtatgtatc aatgtcgact tctattcggg
    11041 ctttgtttat tcctgcatcg gaataccaaa ggaattatat accccgcttt ttgccatggc
    11101 aagaatagcc ggatggtgtg cccaccggat agaggaactc accttccagg caaaacgcat
    11161 cataaggccg gcatttaaaa atgtttacgg caccagggat tatattccca tagatgcaag
    11221 ggactgaccc tgtattctgt gcccgcagcc agccaggcaa tgctgctgtt ctgaactacc
    11281 agtcagcaat acaggacaga gactcaggcc tttcaatcag taattctgct ccagcagctg
    11341 catgtatgat ttcggataaa ggcaggcagg acatttctca tatgcctttt ccgattcata
    11401 tacaaatccg cagttaatgc atttccacca gacctttccg tctttcatga ataccttgtc
    11461 ttccgaaata ttctgaagca gttttaagta tctgcgttca tgttctgctt ccacctttgc
    11521 aatcattctg aaagcggctg ctatttccgg gaaaccctcc tcctttgcca cttcggcaaa
    11581 atgaggataa agatcattcc attcctcatg ttccccttcc gcggcagcct tcaggttttc
    11641 agaggttgtg ccgaccctgc cggccggata tgaagcactt atttcaacca ttcctccttc
    11701 aaggaagctg aaaaatcttt tggcatgctc cttctcctgc agtgcagttt cctgaaaaat
    11761 atttgctatc tgctcaaaac cttcctttct ggcaaccagg gcaaaaaatt cataacggtt
    11821 cgtggcctgt gattcacccg caaatgcctt cagcagattc tgctctgttt tcgtaccttt
    11881 taataatagt gacatagaat tagtttcctt ttggtttaac ttgtttaaat ttgtctgcca
    11941 atataatttt ttttaagctt cggaaaaaaa taattgttcg caatgatctt tcataccggt
    12001 tgccaacagc aatcttcatt caataaacat cactgacatg aaaaagtatt tttggataat
    12061 tccggcactt atactggcat taagtgcatg cagggacaat cagaaggccg tcacgcccga
    12121 aacagctctc ggggcttata ttaactcggc tgatgatacc tataagtggg agataaggga
    12181 ggagttcagt tcggatggcc tgcagacagc tatcctgaaa cttacttccc agaaatggcg
    12241 tgaaattgtc tggaaccatc agctgaccgt gatttctcct gcgggaacac catcagacgg
    12301 ggccttgctc ttcattaccg ggggaagtaa caggaacggg gaaccaaaat ggcattcccg
    12361 gaacaacgag gatttcaaaa tgttcagtat gattgcagca aagaacaatg cagtggtggc
    12421 aatactttcc caggtgccca accagccgct gtatgatgat ctgacggaag atgccctgat
    12481 ctcgttcacg cttcataatt ttaagaatga cagggatttt acctggcccc ttctgttccc
    12541 gatgacgaaa agcgcaataa aggctatgga tgctgttcag gagttttcag aacagaaact
    12601 gaaccataag atccgtggat ttgtggtaag cggtgcttcc aagaggggat ggacaacctg
    12661 gcttacggga tcacaggaca aaagagttct ggccatagcc cccatggtga ttgatgtgct
    12721 taatatgccg gtaaatgtgc cttatcagaa agaggcctgg ggtgattaca gtgaagagat
    12781 aatggactat gtgagactcg gtattgcaca ggatcttgaa agtcaggaag gcagggacct
    12841 tgccacaatg attgacccgt attcataccg tgcctccctc acaatgccca agctcataat
    12901 tataggtaca aatgatgaat actggccggt tgacgcagta aagaaatact tcagtgacct
    12961 gcccggggaa aaatatatcc actatgagcc caacactggc cacggactgg gtgacggagt
    13021 gaatgtggca aagtccctga gcgccttttt taatatcaca atcaataaaa aaagccatcc
    13081 ggtctgcgaa tggcaggtgg aaaatgatgg tgaaaggacc gctctggttg tcagggcatc
    13141 tcctgaactg ataagggccg gcctctggat atgtgattcc cccgaccgcg atttcaggga
    13201 taaccggttc attgaaaaga atattgaata tgagaatccc ggttcggtaa aggtgacggt
    13261 cgataatccc cctgcaggat tcagggcatt ctatgtcgag cttgtttatc cggatccggt
    13321 tgagggaaca tattcaagaa gcacccgcat gtttgtggcc gacagcggtg aattattcct
    13381 ggactgacaa attgttcctg aactgacagc tgccgccgga aaccggacaa aaacattatc
    13441 cggccgtgat gcctatggca tcatgaaagg catcatcatc atgcctcccc cggaattgcc
    13501 gaatctcgga ttcacaacat tgttgtagat tgaaccgaag gtataggtaa atccgaagct
    13561 tgtgaagtat tcgaactgtg ttgcaagttt ctttctctga agcaggatct ggtccagcgg
    13621 catgttctga cgtaccagtc caagctggtc atgcaccatc gagccgcttc ccatgaagga
    13681 aaccgtcaag cccctggcaa ttctgagatc caggtaggca gtaagcgaca gattgttttt
    13741 ggaccagtcg tggaaataat tcctgtattc agccacaagg ttgatattgc cccatttctg
    13801 tactatttca taggatgctg aaagggaatg actccacagg ttttccctga tcctgtcata
    13861 tattgttgta tctatgtaat tgacaaaggt gtagcctgct gtgtacagta tcctcagctg
    13921 acgcctggtt gattcggaat agggataaat atcatattca ataccgggca tgaaggaaat
    13981 actgatgttc tcattgttga atgaagaaga ccgcagtttt gccctggctc ctgcagacca
    14041 gtggttgctg atgcttttca ccaccagagc atccaggttt ttagatgaat tggtacccct
    14101 gatggttatt gagtcattga tcagatactt tgtctcattc tgattcagtt ccgacagcag
    14161 gttaatcttc cagttttcag taatacggct tgctgtaagg cttcctgaaa ggttcctcga
    14221 ccggaaggtt ttttctccct ggagaaaccc gctgacagaa gacctgaaca cccagctgtt
    14281 ccacttgtct gtgtagatag tctcaagcag gggttctgtg aacgatatgt ttatatagcc
    14341 tgcaagaggc gtccttgcaa catatctcat caggcccatt tttaaattgt taacctgctt
    14401 gtacctggtt atttccgaag tttcatcagg cgcagaagtg aaggtcagcg tatcggccat
    14461 tcctgcaaat ttgccctggc ctaccaggaa ataggtgtat tcagtcccgt aagatcctgt
    14521 attctgcctg gtcgaaataa tatacacatc cgcatccttg atctccctga cgtaattgac
    14581 gtagggaatt tccttcctga tgaaatctga tgactccatg taaatattaa tcgcattctt
    14641 cctgagagtg tcagccggaa agacagccct ctgggaatat gactggagaa tgatcatcag
    14701 aaaagcgaga agcagcaagg tttttttcat atccgtcagt attaaacatt ttgtaaccgg
    14761 gtcgaaaata agtaaatatg tcatcctgca atgacggaat catgtgttta acaaatcatt
    14821 aacccggaca aaaaatccgg cacccatgga caggcgccgg aagtaataaa aatttaagga
    14881 atgaaaatac agaatatcag ggatttgtct tctgttctga atcaaggtct tcaagtccct
    14941 ttcttgtcat gatcatagta ctttctgaat aatcattaac aaagtcctta ccgatgtttc
    15001 ccccgagcat tcttcttatg ccgctgtcaa tgctgatttt tgttccccgg ggtacgttta
    15061 catgaacatc aacaacatca aatgaccatt ttactgcttc cggataagta aaatactcat
    15121 caaggaagag ggtatcccct gagattctgt aattatactg aatcctgtcc gccttttttg
    15181 cagcatcggt ttttgatttc cccgatgagc gctttgttac atcaatccat gctgattttc
    15241 cgtcggctgg cataatatcc agatgcgctc cgatatacaa ctgtttattt acctcatcaa
    15301 taaatacaaa ataattcttc cccgggatgc ttatttcatt gtcagtatca agtgcggcaa
    15361 ttcctgaggc ctgttttata taaagagttt caggagggcc cgtaaggtat tccctgtagc
    15421 ctgaacgcgc agtctcaaca aaactgatgc cttcattgaa caggagaatc gacaggatgg
    15481 taactccgag aatccatatc acaaatcccg cgaggaaaaa aatgccatcc gctgccctga
    15541 gccggaatac catcttaata ccaaggtata ccaaagccag aaggggaaga atgacagtaa
    15601 tgacagtaag gaagctgatc cacggcacca ttttcggatt tattacataa ttcagaaatt
    15661 caggaaggta agaaatattt atgccggcag ctcctggcgc ataaattccc ggaaacctga
    15721 aaacaaatac cattataaag gctgccgccg caaaaaagcc ggccacaata aaagacaatc
    15781 cggccaggac aataactgcc ctgatcacca gccagaatat gtttccgaga gccctgaaca
    15841 tttcattaaa ggcatttccc gccctggagg attcagtgaa atcctccggt ttacccatga
    15901 tgagaatcat ttcatcaaca tcagagctgt ctattacact tgctgctgat ttttttgata
    15961 gaaatatttc tgcaatcctg agttcaatat cttcaaccgt ttcattccag ccgggagtgt
    16021 ttctcatttt attgctcagg gccaggagat aatccctgag tatcctgtaa gcttcttcat
    16081 caatgtgaaa aaggattcct ccaaggttga tattgattgt cttatccata attagcctgg
    16141 tttagttttg taataatgac tatttcgcac gaagcatgct gactgactct accagttcat
    16201 cccacgacct gtcaagatct gccaggtaat tcctcccttt ggctgtaagt tcataatact
    16261 tcctcggcgg tccctgctgg ctttcttccc acctgtaact gagcaatccg gaattcttga
    16321 gccgggtcag gagagggtaa agagtacctt caaccacaat tacctcagcc ttcttcagtt
    16381 cattgattat atcacttgag tagcttgatc ccctggaaat tactgacaga atacaatatt
    16441 ccagaatccc tttccgcatt tgagcctttg tgtttaccag atccataaca acagatttaa
    16501 tttttttgcc taaaccagat ttccccttcc tccctacagc ttcatgtttt tcctcaccct
    16561 gtcaaactca tcccgcacgg catcggtaat atttttgata tttaccggat cgcccttcat
    16621 ctcaatcttt tgtgaagtgg tccgtgcctc gggtattgcc acatacagga taagatagat
    16681 gatgatcccc aggccgccgg caaggatcag ggcaatgaat ataatcctga ctatccatgg
    16741 atccagactc cagtaggcac cgatacctgc acagacaccc cctattatcc tgcggtccgg
    16801 atcgcggtac atcctgtgat agccggaaga ggaatccctt tgctttccgg aattgcttcc
    16861 ctcttccgaa aaatcttcag gcctgccaag taccgaaaca gcatagcgga catcctccat
    16921 tgtgattacc tgcctgtaag cactgagtct tgcacggaat aattcagaca tcctggcctc
    16981 aatgtccgag agaatttcgg aagaactctc ctctcctgca aagtgtaatt caagctccct
    17041 gagatatctt ttcagttcgc cgtaggcatc ttcatcaatg ttgaaggcat aacccccgag
    17101 attaatgctg acagttattt tcatcttttc taatatttat actaccatta acaacataca
    17161 ataatccatt acaaagatat atataatttt tactactatg taataccttg tattgttttt
    17221 ttttactttt tccgtctgtc cggttcccgc gggtaatcat accgccggga acaatcagtt
    17281 gcggataaat taaaaaacct ttcccggaaa cattaaaaca agcaattatc catatcttta
    17341 gttccaaaat ttatctgctc atgaagaaat acaaatgggg aatcctggca cccgggaaaa
    17401 tgtcggcaaa gtttacgaaa gccctgaaaa cacttgacaa tgccgagctc catgcagttg
    17461 gctcacgcga cgttgagagg gcggcacttt ttgccaggga ttacggtttt aggaattatt
    17521 acggaagcta tgaagaactt gcagctgatc cggagcttga gatagtctat attgcctctc
    17581 cccactccca tcactatgaa cacactatgc tgtgtctgag aaacggcaag catgtgatct
    17641 gcgaaaaggc cttcgccctg aatgccgggg aagtggctga aatgattgat gaagccggaa
    17701 aaagaaagct cttcctcatg gaggctctct ggcctccctt tcagcctttt tatcagaaag
    17761 ccaaagagat actcagttca ggcatactcg gacggattaa ttacatgaac ggatattttt
    17821 ccttcatacc accctttaat cctgaagacc gcaagttcaa tcttgccctg ggaggcggct
    17881 ccctgcttga tatcggtatt taccctgtta ttgacgcact tacatttctt ggggtgccct
    17941 ccgatataaa agcctctgcc atattcggac aaagcggctc ggaagaatca ctgagcgtta
    18001 ttttttcata tgataacagg tgcatggcat ccatctacag ctctttcagg acctcggcgg
    18061 gcatcggcag tgaactgtac tgtgaaaacg gcaatatgac ggtagcccgt ggcagggata
    18121 tgaatcagac ggttattctt accccccatg acagggaaaa ggaggagttc gtattcaggc
    18181 cgccggccat gggatatcac tgggaagcgg cagaggttat gaaatgtctc gacgaaggca
    18241 ggactgaaag cagtatcgtt ccgttgtcat tcagtctgga tctgataacg actcttgacc
    18301 gcatacgcat agctgccgga atagaatttc ccggcagcgg ataataattt cagcatgtcc
    18361 ggtgaaaacc attcttccct gtcagaagaa gccttcctga aaaaaaagtt tcttctgagt
    18421 ctcattattc ccgggatttt tgtgtttctg atggtactgg ccaaagtaat tgaattgctc
    18481 tttgaaacag atctttccgt gctgggaatt tatcccctgt cattcagggg tctgcccggg
    18541 atcatactat cacccttcat acatgcggat ttcagacatc tgctgaacaa ttccctgccc
    18601 ctcttcctcc tgtcggtatc gctcttctat ttttattcgg aaattgccct gaaggttttc
    18661 atcatgacct ggatctttac aggcctgctt gtgtggaccg gaggcaggga agcatggcac
    18721 atcggggcaa gcggaattgt atacggactg gctgcattcc ttttcttcag cggcatcctg
    18781 agaaaatact acaggctggt agccctctcc ctgcttctgg ttttccttta cggagagatg
    18841 gtctggggat tgtttcccgg catctataaa aacatctcat gggaatccca tatgctggga
    18901 ttcatatccg gtattttcct ggcaattatc taccggaacg aaggcccgca gaaacctgtt
    18961 tacgactgga tggaagagga ggacgaagat gaggaggaag aagaaggggg aaaaaggacg
    19021 gaagagtaaa aggcagaagc ttcatttttc gggtaattta tttcttttta tttattttta
    19081 acgcaaatac taaacctgca ccgaccatga taagcattga aaaaaaatta aacccggctg
    19141 atctgtcaga caagcttgcc ggattctggg aaatttccgg acggaaaatc aacctgattg
    19201 aaaaatacta tgatgaatcc cagggttcac ccgtgtttac ggtaaagggc agatattcaa
    19261 caaggggctg gaccgaatgg acccagggat ttcaatacgg atcagccttc ctccagtttg
    19321 atgccacagg agataaaaag atgctggatt atgccaggga aaagacagtc aggaagatgg
    19381 cccctcatgt gagccatacg ggagttcatg accatggttt caacaatgtg agcacttacg
    19441 ggaatcttct gagactgatg aatgaaggca aaattccctt cagcgaatgg gagaaaaatt
    19501 tctgcgaact ggctctcaaa gtttcaggag ccgttcaggc aagccggtgg actgccatca
    19561 gggatggcgg atatatttac tcctttaacg gtgcccattc attgtttgtc gataccataa
    19621 gaagctgccg cattctgatg attgcccacc gtctcggcca tgttcttcag gctgagggtg
    19681 atatcaggat cagcctgctt gaacgcgcac ttgaacattt gaagtccact gccaggtatt
    19741 cggtttatta tggcgaggga cgtgatatat gggatactga acccggacgt actgcacatg
    19801 aatctgtatt taatcctgct gacggaagtt tccgctgccc gtcctcacag cagggctata
    19861 ccggttttac aacatggacc cggggactgg catgggctgt ctgcggattc tccgaacagc
    19921 ttgaatggat agattttctg aaaagggaag gaacggatca tcccgcagat ctgaataaca
    19981 ttgaaaaaat cattgacagg gcagccaggg tcacatgcga tttttatata aaaaacagtc
    20041 cttccgacgg agtcccctac tgggacaccg gggctccggg gcttgtttat ctgaaagatt
    20101 acctgaacaa gccttcagat cccttcaatg aatatgaacc ggtcgacagc tcggctgcag
    20161 ccatagctgc ccagggtctg ttgcgcctcg ggcactttct gaagcagaag ggggatgttt
    20221 attccggaag ccgctactgg caggcaggac tgaaagtgct tgattcactt ctcagtgaac
    20281 cttatctgag caccggggaa gagcatcagg ggcttctcct gcattcggtt taccacaggc
    20341 ccaacggatg ggatcacatt ccggaaggca gcaaaatacc caacggggaa tcaagcatgt
    20401 ggggagacta tcacatacgg gaagcctgcc tctatcttca cagaatcatc agtaatgaaa
    20461 catattataa tttcttcagc tatacaggat gaatttacaa ccaatcactg accttcagag
    20521 gctctgtatc cacaccataa ccgtaaaacc atggaatatc ggggaaattg ccgcaaattt
    20581 ctcctctgca ggcgtaaaag gtattacagt atggcgggac accctggctg gcagggatat
    20641 acggcaaacc ggcaggatgt tgcgggacca cggtctttcc atagtctctc tctgcagggg
    20701 aggttttttc ccttcagccg acagagccag gcgccgggcc gcaatagatg acaaccgcaa
    20761 agcaattgaa gaagctcatg aactgggaac agacctgatt gtcctggtct gcggggcaga
    20821 tccctcacag tctcttgaag attcacggaa acagatacac gacggaatag catcagtcct
    20881 tcctgatgct gaatccgcag gagtcagact ggcaatcgaa cctctccatc cgatgttcgc
    20941 ggatacacgc tcggcaatca acacccttgc ccaggcaaat gatcttgctg aaatacttga
    21001 aagtccctca gcgggggtgg cagtcgacgt atatcacctg tggtgggacc cggatctgga
    21061 aaaggaaata aagcgctcgg ggaaaaacgg aaagctgttt gcctatcatg tatccgactg
    21121 gaaatcgcca accgtggata tgcttaatga caggggactg atgggtgagg gatgcattcc
    21181 cctgaagaaa atcagatcct gggtggaaga cagcggattc agcggattca ttgaagtgga
    21241 aatcttttca gatatatact ggagccggga tcagcattcc ttcctgcagg atatagtttc
    21301 ctcatacaaa aaatatgtat gacagcattg ccaccggagc cttattccgg actggctcac
    21361 ggcagggacc aatgaatcag acagcagaaa taacatcata tcaaattaat ttaccatgaa
    21421 acagcatacc gtaggaataa tcatgaacgg agtgaccggc agaatgggca caaaccagca
    21481 tcttctccgc tcaattgcgg agataatcag acagggaggc gtaaaatgcg gatcctcaga
    21541 aaccattatg cctgatccca tcctggtggg acggaacgaa acaaagctga aaaacctctg
    21601 caacatttcc ggaatctcaa aaatgagtac cgatctggat gaagtactga aggacaggaa
    21661 ctaccagctt tattttgatg cacaaacaac cggaagaagg gttgatgccg ttaaaaaggc
    21721 catcagggcg ggaaagcata tttactgtga gaaacctgtt gccatcagca cggaggctgc
    21781 cctcgaacta tatgaattat gcagggatgc cggactaaaa aacggagtgg tacaggataa
    21841 actctggctt ccgggaatac tgaaactaaa aaggcttatt gaaacgggat tcttcggaag
    21901 gatcctttcc gtaagggggg aattcggata ctgggtgttt gagggagaaa ccattccggc
    21961 acaaagacct tcatggaatt accgcagcga ggacgacggc ggtattatac tggatatgct
    22021 gtgccactgg agatatgtcc ttgacaatgt tttcggaaaa gtaaaagctg tatcatgcct
    22081 tggagcaacc cacataccgg aaaggttcga tgaggaaggc aaagcctata agtgcactgc
    22141 cgatgatgcc gcctatgcaa ccttcgaact tgaaaacggc attattgcac atttcaattc
    22201 ctcatgggcc gtaagggtac gccgcgatga cctcctcacc cttcaggttg acggaacaag
    22261 gggatctgca gttgccggac tcagggaatg ctatatccag cattacggca acactcccaa
    22321 acctgtatgg aatcctgaca ttccccagcc gattgacttt tttgagggct ggtcaagggt
    22381 gccggagcag gagaattatg acaatgcctt caaggcacag tgggagctct tcctgaagca
    22441 tgtggtgaaa gatgaaccct ttccctggga tcttcatgaa ggtgccaaag gagtgcagct
    22501 tgcggaaaaa ggtatggaaa gctggaaaaa gcgctgctgg acagaaatac cggaactgaa
    22561 aaaatgataa tcaggcccgt tttgtggtaa ttcaccacaa ttattatctt taggtcctga
    22621 taacctaaat taaacctgct aattcagcca gtgatgaata ataaacctgt agctgtcata
    22681 acgggtgcca gcaggggaat cgggagagca attgcaattt ccctggcatc tgaaaagttt
    22741 gatattgccg ctatcgcccg taccgtggag agcgaaggta tggaatccct tgcaagggaa
    22801 ataacagcga aaaatgttgc gtttttcccc gttggccttg acatcgcctg cacggattgc
    22861 caggatgaag ttgttaagaa cattctcggg cgatatggca ggatagacct gcttgtaaac
    22921 aatgcgggcg tggctcccct gaaacgtaac gacctcctcg aaatgaccga ggagagctat
    22981 gaaagagtca tgaacataaa cctgaaaggc cccgtctttt ttgcccagcg aatcgccagg
    23041 gaaatgattt atctcggccg gcagatttca gactacagac ccgcaatcat ctttatctca
    23101 tcaatatcat ccaccaggac atctgtcaac agggcggaat actgcatttc caaagccggt
    23161 ctgagcatgg cttcctcggt ctttgccgac aggcttgcag ctgacaatat cagggtgttt
    23221 gaaatccggc ccggcattat caataccgat atgactgcca gggtaaagga tacatatgac
    23281 aaactgattg ccgacggcat ggttccccag aaacggtggg gctatccgga agatatcggc
    23341 agggcagttg catctctcgc acggggagac tgggattttt caaccggagc aatattcgat
    23401 atcagcggtg gccttaatat cacccggctc tgacaggaat actgaccttc agattattcg
    23461 gccacgggca gaaatacttg ttgaacattt tcacgaatat tctgttaatt cctgaaatca
    23521 aatgatgaat gttatgaaac cgtttttatt aacttttttt attttattgc ttatgaatac
    23581 tcctaccaat gcagaggaaa agatgaatgc cgatgtcagg aaaaccactc tgaactggca
    23641 tggggagaaa gtcctcggtc atcataccgg aacgattctc ctcagggatg gctggattat
    23701 ttttgataac aacagaatta cctccggaga atttacaatt gatatgacaa caattaagga
    23761 tgacgattcg aatgaaagac ttgagggtca tctgaaatct gatgatttct tcggagtgga
    23821 caaacaccct ctgtccaaaa ttgtgatcct ggaaagcgaa gcttttgaca agggcaaggg
    23881 tctggttaag ggccatctca ctatcaaggg gataacacat ccggttgaat tcagcgccac
    23941 aatgcaaaag aataatgaag gaacaagctt ttttgccctt atcattgtcg accggtccaa
    24001 gtataatgta cgctatggtt ccggttcatt tttcgagaat cttggcgata aagcgattta
    24061 tgacgaattc agggtcaggg tgaatctgca tctgaactga tagccataac aaattacacg
    24121 ggaattgcaa tgatttccgt atcacctgat acagcttcag gattaagaac cctgaagctg
    24181 tatttatttt tcctcagaaa attctccgtg aagtccctgc attccttccc atgagtggca
    24241 acaagcattt tcggatgttc ccgtgaaatg aattcctcca gatcggcaag cacttccatc
    24301 tcaccaccct caacatctat ttttataaaa tcaggcggcc ggatcacgcc ccgttttatc
    24361 agtccgccga cagatacctg ttcaactttc aggtttccat ctcctgtcac aaaacctgtt
    24421 gcggaaccgt gccggttgtc aaaacggaca aatccgtcat acctgccggc tgccgcttca
    24481 atcagggtga tattcctgaa atcatttatt tcaatatgcc gcctgaaaaa cttaatattc
    24541 atcggtctcg gctccagtga atagatccat ccttcccccc tgctgactgt tgcagccatc
    24601 gaggaataat atccgaaatg tgccccgata tccaggaata tgcttcccgg ttcgaaatta
    24661 tccaggaagg ctctggtttt gtacgactca taatcacctc tgatgaatct ggaacccgag
    24721 gcaatactcc attttttccc tttgagtggt ccggaccgta ttcttgcgga tactctcctg
    24781 aatacagttt taaagacgtg cttaagcttc atttacaaaa gtaaaacagg gggaaagtta
    24841 ataaaattat cagcccctga aatgaatttc ttttttagaa aattattaaa actcttggaa
    24901 atacaataaa tcataataat cttgtattga taaaacatat tcatctatgg caaatagtgt
    24961 ttctcttaaa aacgacaggc aaaaacttgt cacagaaggt tatctcatcc cttttatgct
    25021 ggttaccagc ttgttttttc tctggggctt tgctcacgga tgcctcgatg tcctgaacaa
    25081 gcattttcag gaattgctta gcatttcaaa ggcaaaatca gctcttgtgc aattcgtgtt
    25141 ttacggtgga tatttcctga tggccatccc cgcaggcctc ctgatgcagc gcttcggata
    25201 caaaaaggga atcatatggg gcctgctcct tttctgtgcc ggtgctttcc tgatgtttcc
    25261 ggcaactttt atccagacct tcggcagctt tcttgcctgc ctgttcataa ttgcctgcgg
    25321 actaaccttt ctcgaaacag ccgcaaatcc ctatacaaca gtacttggtc ctccggaaag
    25381 tgcagaaagg cgtatcaatt tctcccagtc attcaacggt ctgggatgga tagccggacc
    25441 tctcatcggt ggactgctca ttttttcgaa cactgaaagc gaaaataaat tctcatccat
    25501 tgccctgccc tacatggtga tcggcactct ggtccttctt gtcgcactgc ttttctggcg
    25561 ggtcaccctt ccagaagtaa aggaagaatc acaaaaagcg gatgacaatg gaccggacag
    25621 caatgcggcg gattatgatg tgagctggag aaatctcttc cgtcacaggc atttcgtcct
    25681 ggcagtgatt gcacagttct tttatgttgc agcccagacg ggagtgaaca gttttttcat
    25741 caactatgtt acggaagaaa tgccggccat aagcaaccag gcagcatcca ggattcttgc
    25801 tttcggaggc atgggacttt tctggctggg aaggtttacg ggaagcacaa ttttcatgcg
    25861 gctggcaaaa ccaaacaggc tgctcgcgat atatgccctg atgaatgtta ttactatgat
    25921 cattgtaatg gccgggctcg gatggttttc cgttgtggcc ctgttttcaa catacttctt
    25981 catgtcaatt atgtttccca caatatttgc ccttggaata aaggatctgg ggccactgac
    26041 caaaaaggca tcatccttcc ttgtaatggc aatagtggga ggcgctctca ttcccatgct
    26101 gatgggtcat attgcagata tttccaccat ggcaacgggt ttcgcagtac ctcttgcctg
    26161 ctttgcattc atcctgtatt tcggccttga cggatataag gtaagaattg ccggaaagta
    26221 aaccatcatt aaccatactg ataaacaaaa ttccgttagt atcttaaaaa taaattgaca
    26281 ggcaccccga agaaaaacct gaggatttca tttgtcacat ccggacactt tcctgatgac
    26341 gacaggattt tttatcatca gggcagaacc ctggcagaaa aaggattccc tgttcagatt
    26401 gtttcctcta aaacagatac tgactgttcc gctgagggaa tgatcctgaa ctgcttcgac
    26461 gggggatcca tgactaaaag ggaaaaaata aggaaattca ccgaaaccct caggcagttc
    26521 aatccggatg tactgatatg ttcagaacct cttacggtac tggcaggcag attattcaga
    26581 cacgggagta aaaaaaggat cagaataatc tatgatatta ccgaatggta tccttcaaag
    26641 aaaaacctgg ccgggaaaag agctttctgc aaatacatca cttttatcaa acttttgttt
    26701 ttcaatatat tatcctcagc actggccgat gcgtttattt tcggtgaata ttataaaagc
    26761 agaccttaca gattcctttt ccctttcaga ccattcattt tctcaaccta ctatcctgat
    26821 cttaaataca ttgaatttaa aaaacccgag ccggccgacg ggaaactaag gctctcctat
    26881 tcagggaaaa taagccggga gaagggattt gtgaatttca taagtgtcat agacggcctt
    26941 tcggctgttc accatgatct tgagattgag gtgaaaataa caggctggtt cgaaacggat
    27001 gatgacagga aagaatgcga aacatacctg aaaaacgtga atgcggatat tgctttcatc
    27061 atgaagggaa aacaggattt tgccggatat accgggagtt tgcatgaaac tgatatcttc
    27121 ctcgatctca gaagtgatga ccctgaaaat aaacattgcc ttcccataaa attattttat
    27181 tatgctgcag ctggcagacc ggtgattttt tctgacctta aggcaatcag aaaagaagtg
    27241 gaagtggaga aattcggatt cctcgtcaaa cccggtgata ctgaaatgat tgtcagactg
    27301 atttcggaat atctcgagaa tagtgactta tactgccgtc actgcaatga agcacgaaat
    27361 ctggccgaga caaagtataa ctggggaagg atcagttcag agtttgttaa attcgttatt
    27421 aatgcagctt cccgcacggg agccgctgat aaataatctt gaaatgttat ccaggaacac
    27481 gtcaattacc atcgttacca aattcctgat tctggcggcc aactttgccc ttgtggttgc
    27541 cagtaccagg ctgtggggca gtgaaggcag gggcgaaata gccctgttgc tggcaaacat
    27601 ttcaataata gccatattcg gaaatgtgtt ttgcggaagt acaatagctt tccatacacc
    27661 ggtaatgaac agggatattc tcctgacagc gggatccgcc ggagctcttc ttgtgtccct
    27721 tacaggttcg ctgttctttt cgctgctttt cggattcagg aatttcacct ttctgcttcc
    27781 aatgtccttc ttcctgtcac tttcaacact cgttacatca taccggctgg ggaaaaaaga
    27841 ttttgtgaga tacaacctgc ttaccctgct gaaccccctg tccatactca tctttttata
    27901 catactgtat gcactgctgc ataaaacagg tcttgaaact tattaccatg cctggtatat
    27961 cggtacggga tcagtctccc tcttcgcact gacaagcctc ctgagaagaa atccgtataa
    28021 aaggcccggt ctgaaaactg ctgaccttaa gagaatactg aactacggtt ttaaaaatga
    28081 attcaattat ctgctacagt tcctctcata ccgcctttca tattatttca ttgcacggat
    28141 tctcggatat gtacagctcg ggattttttc ggtagtaatt tccgtttctg aggcagtctg
    28201 gattatcagc cggagcatgt cggcagttca ttattccaat gtcatcaaca gcaatgattt
    28261 catcagaagc cgcaaagaga cagtcgcttt cgcaagacaa agctttataa tcagcattgc
    28321 tgtcctggca gtttccgttc ttgttccggt gaaagtatat cagctggtct tcggggctga
    28381 attcggtatc gtcagaaaat atattctgta catgataccg ggcataatgg caatctccgt
    28441 atcgaacctc tatggacatt actttgccgg aacgggaaag ctgaacataa taaggaacaa
    28501 atcactcata ggccttctgg caataattat atccatgccc tttctgataa aggcattcca
    28561 gatggaaggc gtatgcattg ctgttaatat ttcatatata ctatcttcgg tttatctctg
    28621 ggtttatttc cgcagggaag gaaaaagcct taactttaaa actcaagcct aataaactat
    28681 gaaaaagcat ctcttcatat ttgcagtttt cctgatatcc ggatttacaa acttcagtga
    28741 agcatgtacc acttttatta tttcaggaag atatactccc gatagcaggc caatcctgta
    28801 caagcaccgc gacaccgggg ttactgacaa tgccctggca gttttcagtg acggaaaata
    28861 taattacgtg ggtctgctga attcggataa aaactggaac aatgaagtat ggggaggatt
    28921 caattctgca gggtttgcaa taatgaactc tgtcgcctat aacaagaaca tcggtgatac
    28981 caccggcctg gccgatcagg aaggaaggat catgaaactt gcccttcaga actgtgccac
    29041 cgtggacgat tttgaaaaac tcctggcaga acttccgaag cccctgggtg ttgataccaa
    29101 tttcggagtg atagacgcac tcggaggagc cgcctatttt gaaacgggaa attacggctt
    29161 tgaaaagata gatgccaatg atgctgcagc ggcaccctac ggctatctta taaggacaaa
    29221 ccatgccttt accggacctg tcgacaaggg attcggctac atcaggtact caacggcaaa
    29281 tgaagccctt tacagggcag ctgcaatgaa caggtacgat ccgcagtatc tcatatcaaa
    29341 catatcccgc aatctttatc attcacttac ggatgtcaat ctccgggatg tactcccggc
    29401 agacagtgaa cgggagaaat ttgttttttt cgaggatttc attcccaggt attcctccgc
    29461 atctgctatc tgtgttgctg gtacaaaagc gggtgaagat ccctcaggca cggtaatgtg
    29521 gactttatgc ggctttcctc tcacaactgc cgttgtgccc gtctggctga caaaagataa
    29581 aatccttccg gcagcggtga gtatgaagga tgatcttcat tccccgctgt gcgacgcggc
    29641 actcaggctg aaagacagat gcttcccggt aaaaagagga tccgggacga aatatctgaa
    29701 tcttgcggca cttctgaacg gccggaacac cggaatctta cagctgctcg aatctttcga
    29761 gaatgaaata ttcaaaaagg cggatgaact gatccgctct gcacccggca tgataccgga
    29821 tgaaaaaggc attggccggt tttacagctg gctggatgac tatattgaag attcataccg
    29881 tatgctgtcc gaaaccggaa cagcaggcgg acagaaactg cccccggagc ttgttgatcc
    29941 tccgcgcgaa ttttcagtta tgcccttctg gttctggaat gataccctga aagatgatga
    30001 aatcatccgg cagatcgctg attttgaatc ccacggagtt tatggttttg tcattcaccc
    30061 gcgcgtcggt ctgcctcaga atgtgaaatg gctcggaccc gaaatgatcc atgcaatgaa
    30121 tattgccata agcgaagcag cccgccgcaa tatgtatgtt atactctatg acgagggaat
    30181 gtatccttcg ggctcatctt ccggacaggt tgtggaaaaa aatccggaac atgcagccag
    30241 gggactggca aaaatcgatc tgaaggaagg agaagaactc cggctggaag aaggctggaa
    30301 gcttgtaacc attgcccagc ggcccggaaa cagacgcacg gctatcatag aacgcccctc
    30361 cggaggggta atccggggtc ttcattacct gaatgagggc gaggaaaggc ttctcgaaca
    30421 ttccccgccc gcgggtgacc tcctcaaccc ggatgcagtg tcaagcttca tttcacttgt
    30481 atatgataaa tatgcaaggg aattcgggaa atatttcggg aataccatca tgggaatctt
    30541 taccgatgaa ccctctccgc tcggaaggga tgccctgagg ggaatggttc cgggaaatgc
    30601 atccctcctt ccgcgtatta aaaagatact gggatatgac attacccccc atctggcaga
    30661 cctctggtat aacgactatc ctgattcaga acggcacagg accgattatt accatgccat
    30721 caacatatgc cttgaggaaa actattacag gagactgggc aactggtgct tccttcataa
    30781 catatctctt atgggacatc cggccggatc aatggacata ggcactcagc gttatttcca
    30841 gatccccggc caggacctcg tatggagata tgttgaaccg gggccgaaag ccctggaggg
    30901 acaacattca actatggcca agggtgcttc aagtgcaatg atccacaacg gttatcgccg
    30961 gaattcaaat gagctttacg gagcctacgg ccatgagctc acctgggaag agatgctgtg
    31021 gcttgccggc tggtgttttg taagaggtca taacctgctc attccccatg cctttttcta
    31081 ttcggtccgg ggtccaagaa ttgatgagcg tccgcctgat gtggggccga atgcatcctg
    31141 gtggccggat tataaaccct atgccgatgc ctgccgcaga ttaagctggc tgaataccga
    31201 ttcccggcat atatgcgacc ttgcaatact ctgcgaagcc acctggctgc ctgacaggcc
    31261 ggcaaaagta ctgtacagga accagcggga tttcaactac cttgaaatcc gccatctgtg
    31321 ggaagacgca aagattgatt ccaggggaat ccatataggg gacatgactt acagggcact
    31381 tatagtcgac agcctttcat tcattccccc cagggtgatt cctaaactta agaaactggc
    31441 caggcacagg cacctcatcc tgagaagtga ctcaaaactt gcatccgtct gcaacggggc
    31501 actggtttac ggaagcgggg atgaactcag ggctgcagta aacaaaatca ccccgcccga
    31561 catcgtcctg agtccttctt caggaaacat acgctgccgc catgtggaaa aagacgggga
    31621 tcattacttc atgctcttca atgaggaaaa ttccgaagta actgcaaaca tcagcctgaa
    31681 aattgaaaat gacattaaga ataccggaaa tacaagggaa tggattgacc cattcaccgc
    31741 aaaaacaagt actgccggaa cagaccgggc ggtatatttc aaaccctttg aaatgaaatt
    31801 gctcagaatt gcccggaaat aacaattata accactcatt aattctgaaa actcatgccg
    31861 tttaaaacat tgaatctctt ttttcacttc tgccgcggaa ttctgccctt agcttttctg
    31921 gtgctgacgg cctgcagcaa ccggcaggtt ccggaagaac tgatggtaga attcatccgc
    31981 gatccggaag gagtcaggat aatggatctc aggccggaat ttacatggat agtgccgctg
    32041 catgcaaaaa accagactgc ctgtgaaata ctggtatcct cgggcaggga aaagcttgaa
    32101 aaagatattg cagatatctg gaacagcgga aaaatgatcg ctccggcatc gtgtgaggtg
    32161 gaatacggag gacctctcct gtcgcctgac agcaggtact tctggaaagt aaggatatgg
    32221 gatgaaaagg acagaccggc cggatactcg gaaatccggt cttttacaac cggaaatccg
    32281 gcgggctatg ccactactgc caacaaattc cgttcgtcat caatacatcc cctgaagctg
    32341 acaaggatca gtgaaggtca ttattttgca gacttcggaa aggatgcctt cggaaaactt
    32401 attattaccg gtatcagtcc tgatatagcc gataccctga ctgtccacct gggggaaaaa
    32461 cttgccgcag caaacactgt tgaccgtaat cccgggggaa caatacgtta ttacagagta
    32521 aaaatccccc tgaaacccgg acaaaattct ttcaccgcag aactgcctcc ggatgaaagg
    32581 aataccgggc cctcagccgt acatcttccc gattcactcg gcatcattgc acctttccgc
    32641 tatgcagaga ttgaaaactg cagcttcagg ctcgaaaaga acaaccttgt tcaggaagta
    32701 accagttatt tttttgatga cggtgcgggc agcttcacct gctccgatac tattctgaac
    32761 caggtgtggg atatgtgcaa atataccatg aaggccacat catttgcggg gatttatatt
    32821 gacggcgaca gggaaaggat cccctatgaa gccgatgcct atataaatca gctggggcac
    32881 tattacaccg acagggaata ttcaatggga aggcttacaa acgaatattt tataaaacat
    32941 cctacctggc ctaccgagtg gatacttcac acagtactga tgttctggaa cgattacatg
    33001 ttcaccggga atcccgaatc actcagggaa tactatgagg agctccggca caaaaccctc
    33061 atttccctgg cgagggaaga tggtctgatc tcatcaaaaa atgcaactga ccggattatg
    33121 gaggatatcg gtttttcaga cccgggggaa aggatacgcg atattgttga ctggccgccg
    33181 gcacagaagg atacgggctg gaagcttgcc ggtccggaag gcgaaagaga cggatatgac
    33241 atggttgaaa tcaatactgt ggtaaattca ttctattacc gtaatctgtt tctcatgtca
    33301 gaaatagccg ggatcctcgg gaaaacagat gactctgcat tctttcgcag ggaatcggaa
    33361 aaggtaagga aatcaatcaa tgaaaaactg ctcgaccggt ccaaaggtat ttacctcgat
    33421 ggtgagaatt cggaacattc atcgcttcat gccaacatga tgcccctggc tttcggaatc
    33481 attcccgcgg agtacagaaa aagcgtcatt gagtttatca aatccagggg aatggcatgc
    33541 agtgtttacg gagcccagta cctgctggag ggattgttca gggaaggcga agcggaatat
    33601 gcagtcagtc tgatgaatgc aacccacgac aggagctggt ggaacatgat aaggaccgga
    33661 tccacaatgg cgatggaggc ctgggacatg aaatacaaac ccaattccga ctggaaccat
    33721 gcatggggag cagtacctgc aaatatcatt cccggctata tgtggggtat tgcccctgca
    33781 gaacccggtt tcgctgctgt taccatcaga cctcagctca gcggcctgac ctacagtaaa
    33841 attgaagtgc ccacgatcag gggaaaaata gttgcggaat tcagggccaa ggggaaatca
    33901 tccgtttata cggtaaacat ccctgccaat atgaagggca gactcattct tcctgacagg
    33961 gaagcatttg aactcagtcc gggaatcaat tcattgaaac aggttcatac cgtaaaaaca
    34021 caataaaatc aacaaacatc acaaaaaaaa acatacaatg aaaacagcta tttttccctt
    34081 gcttttcaca ttcttacttg tggcggcatg cccgtcctgc agtaatactg atatttacag
    34141 ggcagacatc atcgtttacg gaggaacatc ggctgctgtc acaacagccg ttcaggcagc
    34201 aagaatggga aaatcggtaa ttgttgtttc tcccgacaga catcttggcg gcctgtcctc
    34261 ttccggtctg ggatataccg acacagggaa caaggaagtt atcggaggaa ttgccagaga
    34321 gttttatcaa tatgtttacc aacactatga aaggcctgaa tcatggaaat ggcaggaaaa
    34381 gtcctcatat ggcaaccgcg gacagggaat accggcaata gacggggaaa aacggaccat
    34441 gtggatcttt gagccccacg tggcagaagc catattcgaa aaatttacag aggatcataa
    34501 tatcactgtt ttccgcgatg aatggctgga cagggaagaa aatacaatta aaaaggatgg
    34561 caggataatt tccttccgga cactcagcgg taaaaccttc agaggaaaaa tttttgttga
    34621 tgcaagctat gaaggtgatc tgatggcagc tgccggagta agctatcatg tgggcaggga
    34681 ggcaaacagt gtctatgacg aaacctggaa tggtgtccag aaaggattgt atcatcataa
    34741 acactatttc agggataata taagcccgta taaaataccg ggcgatcctt caggcggact
    34801 gcttccccgt gtttctgccg gggcacccgg tgaaaacgga tccgccgaca acaaaataca
    34861 ggcatactgc tacaggttat gccttacaaa gcatcctgaa aacaaaatac ctttcaccag
    34921 gcctgaggga tatgacagca cacagtatga actgctggtc agggtactgg cagcaggctg
    34981 ggatgaatat tttgagaaat ttgacgagat acccaatctt aaaaccgatg taaacaacca
    35041 cgggcccttc agctcggata atatcggcat gaactatgat tacccggagg catcctatga
    35101 aagacgccgg gagatactga atgaacatat aacctaccag aaggggctgc tctattttac
    35161 tgcaacggat aaaaggattc cggaatggct caggaatgaa ataaatttat ggggctatgc
    35221 ggctgatgaa ttcaccgata acggaaactg gccctataat atatatgtaa gggaagcccg
    35281 ccgtatgacc ggggaatatg taatgaccga acatgatgta cttgtcagaa ggcctgttcc
    35341 tgaacccgtg ggcatgggct catatacaat ggactcacat catgtgcagc gttatgtgac
    35401 acccgaagga tatgttcaga atgaaggtga tataggagta cctgcccctg aaccctatca
    35461 gatagccatg ggctctctcc tgccgaaacg ctctgaatgt tcaaatctta ttgtcccggt
    35521 ttgtgtttcc agttcgcatg tagcctttgg ctcagtcagg atggagcctg ttttcatgat
    35581 tctgggccag agtgccggaa ccatcgcctc aatggccatt gataaaaaca aaaacattca
    35641 cgacctgtca tattccggga taagggagaa actgctgagt gacggacaga tacttgagta
    35701 ttaatcaggg ttcgctaata tgccgtattt caattacatc tgtctcatct gtcttttttg
    35761 atttttcgtc aaatttagaa gctgttcgtc aaagatgctt taaaatgctt gactttgaat
    35821 taactgtctt ctaattttga tttagatatt tactattaac ctcaatgtcc gggaaccagt
    35881 tagtaattcc cattatgagt gcgtagaaaa ttgtagtgca agctgaaaaa cttaagagta
    35941 agcaaaacca aaaaaaacaa ccatgaaaaa aatttatctg tgtattgctg cagcttttat
    36001 tctggctgca tccccctccc ttgctcagtt tgaaaaaggc agtttcatta cgggggttag
    36061 ttcaaccttc ggcgtaggct cattcggaac tgatctgatg agtattggta caaccagtca
    36121 aaaaataaaa tataatgagg aagaggtgaa gggcactctg aaaggcggta agtattttaa
    36181 tttatccctt ttaccccgtg ccggatactt tgtcattgac aatctttcag tcggacttga
    36241 tctgattctg ggttataatt catggaagta tccggaagaa ggcaagaacg gtgttgagaa
    36301 gacaacaagt acttctgtgg cggcaggccc ttttgtaaga tattattacc ccctggagaa
    36361 gctgtaccct tttgttcagg caaatgtggc ttttggaata gataaaacca agggatacta
    36421 cagttcaaca aatttgcttt ccgtgtatgg aattggtgtg ggagctgcca agactattgg
    36481 agagaaggta atggtggatg ctgtgctcgg ctggaattcc gccagcctga cggatgaaga
    36541 caatgacaag gagatttatg gttcatttgg tgtgcgtgtt ggtttctcct attttttcag
    36601 cattaagtaa ccagtattga tttatcagaa ataatttaaa aatcctatca tgaaaaagat
    36661 atatttattt gttgcggcta tttgtgtttt atcggtttca cacacctttg cacaatttga
    36721 aaagggcaga atactggcag gaatttcttc cggtctccag gtcactgacc caggagcttc
    36781 cctgatgaac ctgggattgg gaaggataaa atataaggaa gacggagagc gcgcggaaaa
    36841 aggtcccaaa gaatttaata ttaatcttct accccgtgca gcttattttg ttatggataa
    36901 tctggctgta ggcctggatc tgcaggtata tctgaacgtc ttaaaagaca gaactaccaa
    36961 aggttccaac tatgtcaata cttttctggc agcgggccct tttgtgagat attattatcc
    37021 tctggagaaa attcatcctt ttgctgaggc aaacatggga ctcggcatct ccagaacact
    37081 ggattcctcg gatggtgaaa atggtttttt atacggagtt ggtattggcg ccgcactgcc
    37141 tcttggagaa aacctgatgc tggacggcca ggtcggattt agctcaaata tatggaaaga
    37201 tgtggaccct gacaagaaac aggcttctac caaatatatt tacaactcgt tcggtgggcg
    37261 tgtcggattt atcctgttat tcggtaaggg aaaataattc ataaaattat ttcacggaaa
    37321 cacagcggtt tcagacatat tcttatctga aacaatcagg acgtgtcatc actgattcat
    37381 attccgcgta tcaggcatga taaacctgat tgtgattacc gggttttccg gcacatcttt
    37441 cctgcaggcc ttaattaaaa attctgccac agggttttga ataaattaaa atatttcatg
    37501 gcttaaataa atcttcatat ctgtaatgaa aaaaatacat atgaaaataa caagtatact
    37561 gatgtcactt gttttactta tttcccagag ttacagcctg tcggcaggaa caatccccgt
    37621 tgagttaaaa agccttgaag aatcagtttt catacccgac caggaggcta ttgacctggc
    37681 tttcggggaa ctgaatgaac tggatgaata tctcgagctg aataccggtt taagctacac
    37741 agatctgcag gcagccggca gcattctgat taaggatatc agcgacatgg ttgccccgtt
    37801 tggccagtct gaagaaggtg atgacgagga tccgctggga atacccgctt tctggtgggg
    37861 gtgcttcatg ggaattatag gagtactgct ggtctatata tttactgaca ataacaagga
    37921 ccaaacaaaa aaggcattca agggattcct ggtaacgctt gtttcttcct ttgccgccag
    37981 tgcaattatc atgattgtta tacttgcaac caccccggta gtgttatcag attagaatta
    38041 aacatgaatg aaaaaattat aaaaaattct catcgggtgt tttagcctga ttaattcata
    38101 aactcataat tgagtgtaaa gatacacctt tgggtcttct
//
LOCUS       DAOSPX010000002        35569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_1325_length_35569_cov_4.775581, whole genome shotgun sequence.
ACCESSION   DAOSPX010000002 DAOSPX010000000
VERSION     DAOSPX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 35569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35569
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1325_length_35569_cov_4.775581"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLZ75_00165"
     CDS             <1..574
                     /locus_tag="PLZ75_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOS70841.1"
                     /translation="DGSQRKLLGTVKYRSPGEYLISLRHNTGIEAARIFIKGDSVMVN
                     DRINRNLYIAENDYLQDRFGIGTTLVPLVFGDFLETMTDVVTLKDCRSGVSEIQGYAG
                     NRDIWYCIDCKSGKASSVTVSDRIGRNSVSLSFGEYRKTGYFIYPGRIKIEDNSGLGK
                     ISISVNSVEFRDSQKLEFLPGRNYKKIILK"
     gene            571..1752
                     /locus_tag="PLZ75_00170"
     CDS             571..1752
                     /locus_tag="PLZ75_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOS70842.1"
                     /translation="MKNIVICLMLLVLSNSPALGQSRADLEDQRRRTIEEIGYVDEML
                     KSTERERNQGIQALRIIGNKVSLRETVLKGMNEEIELLTGRIILNKTALDMMESDLQT
                     LKDDYSRSVVNSYKMAKANPDVVYILSARDFNQGYKRVRYLQQVTRYRRNESEIIREL
                     MQEIKGAKERLEKDLERISELQKRETQQKELLQAEQRRQQRMVQNLSNKEKQLRKELE
                     DKKRIARRIEAEIARIIEEERLKAAKSQLSPEQKLIGDNFQDNRGRLPWPVERGIITA
                     HFGVHPHPVLKYVTEENKGIEITSKGKVMARSVFKGEVSAISVIAGANITVIIRHGNY
                     LTVYSNLINVRVRKGDKVDTRQEVGEVYIDPQGDDNAVLKFMIFEEKTALDPEKWIIS
                     N"
     gene            1869..2264
                     /locus_tag="PLZ75_00175"
     CDS             1869..2264
                     /locus_tag="PLZ75_00175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS70843.1"
                     /translation="MQQKIRITSEIDKLRVVENAIDNLTNDNEISQDSYGKILIGTLE
                     AVNNAIVHGNKSDKSKFVEVEFKFEKGNLKVTITDEGEGFDPSGIPDPTLPENIEALT
                     GRGVFLMSRLADEIKFNNQGNSVTMTFKV"
     gene            2272..2715
                     /gene="ybeY"
                     /locus_tag="PLZ75_00180"
     CDS             2272..2715
                     /gene="ybeY"
                     /locus_tag="PLZ75_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOS70844.1"
                     /translation="MRIRFFYDEVKYRLREVKYLKDFIAEVIREEKHSPGDLSFILTG
                     DNTIREINREFLKQDYFTDVIAFDYGKGNNTCGEIYISVDTVKKNAVNYKVSFKNELI
                     RVILHGTLHLCGYRDGTEEDALIMKERENELLNRFLEGKGSGIQI"
     gene            2699..4573
                     /gene="mnmG"
                     /locus_tag="PLZ75_00185"
     CDS             2699..4573
                     /gene="mnmG"
                     /locus_tag="PLZ75_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOS70845.1"
                     /translation="MEFRYDVIVVGGGHAGCEAACAAARLGARTLLLTMDLTKLAQMS
                     CNPAMGGIAKGQIVREIDALGGMSALVTDRSMIQFRMLNKSKGPAMWSPRAQCDRLLF
                     SAEWRKVLERTDNLFLWQEQALKLIIENGRIRGVETEFGTRFKGDAVILTTGTFLNGE
                     MHVGLRKVRGGRAGDSDSAGLSDQLVDLGFKVERLKTGTSARIDGRTVRFEKMTEQKG
                     DEEGGSFSFMSDRKAIKDQKSCFITHTSREVHEELRGGLEFSPLFSGRIRGRGPRYCP
                     SIEDKIVTFADKDSHQLFLEPEGRETNEYYLNGFASSLPLEHQYRAMRKIPGLEEVEI
                     YRPGYAIEYDFFQPTQLKHTLETKRVENLYFAGQINGTTGYEEAGAQGLMAGINAVLK
                     LKGRDNVILGREESYIGVLVDDLVTKGVDEPYRMFTSRAEYRILLRQDNADERLTETG
                     FNIGLADRERLNRLNRKRELVKEIIWFLENKSVSPEEINEFLAEKSSSKIGQKVKAFS
                     IAARPQVKLSEFLNLISQGENLKALRSGRFAEFSEAAEIRIKYEGYIQREKLIAEKLK
                     RLEKIKIPADINYSELLSISTEGRQKLDRIRPASIGQAGRISGVSPSDLNILLMYLGR
                     "
     gene            4979..6598
                     /gene="ade"
                     /locus_tag="PLZ75_00190"
     CDS             4979..6598
                     /gene="ade"
                     /locus_tag="PLZ75_00190"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOS70846.1"
                     /translation="MTISGRIVDIHQRRIFPGDVKIEGSRIKSIAERKDAGDLYIIPG
                     LIDAHIHIESSMLTPASFAVAAVSRGTTGSVSDPHEIANIMGREGVDFMIEDGRKTPF
                     KFYFGAPSCVPATDFETAGARLGPAEIRELLEMEEIKYLAEMMNYPGVIYDDKEVLEK
                     IALAKSLKKPVDGHAPGLSGDALRKYIGAGIETDHECSTLEEAQEKISLGMKVIIREG
                     SAGRNLHALKDLYRTHPSVIMLGSDDIHPEMLMKRHLDKLISGLIREGYSPFDVIASA
                     TLNPVMHYGLESGLLRPGDFADLAVVDDLREMNVLETWINGQKVFDRGKVQFSARPGP
                     AVNRFSCSEICTEEILVKNCGAPFRLISAFDGELITKEVIHEAVQGKMVESNTTADVL
                     KIVVKDRYHDSAPAVAFIRGFGLKSGAFAASVAHDSHNIIAIGVSDQDIADAVNEIVR
                     LKGGMAVVNNGKTHSMQLSVAGIMSDRPCEEVAAEYQFLSGLVRDLGCKMTSPFMTLS
                     FMALLVIPELKLSDRGLFNTSNFSFVPLFAE"
     gene            6657..8450
                     /gene="uvrC"
                     /locus_tag="PLZ75_00195"
     CDS             6657..8450
                     /gene="uvrC"
                     /locus_tag="PLZ75_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOS70847.1"
                     /translation="MTLIPQDIVPLLPDKPGVYQFLDSSGRIIYAGKAKNLKKRVSSY
                     FSKNQSGKTRVMLARAAGLNHIVVDNESEALLLENNIIKQHQPRYNIQLKDDKTFPWI
                     CIRNEPFPRIFSTRNTVKDGSLYYGPYTSALMVKTLLELIRNIYKIRTCSLNLSRENI
                     AAGRFRVCLEYHLGNCRAPCVGLQDEEDYSENVRQIKEILKGNISTVIDHLKSLMMGY
                     AAELRFEEAQAVKNKIDILSKFRSRSTVVSTTIRNVDVFAMTQDTDRAYINYLKVIKG
                     AVIQAYTLEIRSRVDEEKESLLGFAVTEIRQRLSSDSPEIIVPFKPDILFENIKYTVP
                     KKGDKQKLLELAGRNALYYKLEQRKKKEIIPRGDRTLKNLEKLRNDLNMSEMPLHIEC
                     FDNSSIMGQNPVAACVVFRNGRPSKKEYRHFNVKTVTGQDDYSTMEEIVWRRYKRITG
                     EGKPMPQLIVIDGGKGQLSSAMKSLRKLNVGDGTTVIGIAEKLEEIYFPGDSVPIYLD
                     KTSVSLKIIQQVRNEAHRFGITFHRNKRSGDLTKSSLDSIPGIGIRTKEILLKKAGSV
                     DKIRKLSEEELEKLVGRKKAEILRNFLKESP"
     gene            complement(8642..9262)
                     /locus_tag="PLZ75_00200"
     CDS             complement(8642..9262)
                     /locus_tag="PLZ75_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOS70848.1"
                     /translation="MIKNIVFDLGNVLLSWKPGQYLEESGYDSDTVTLIMRDIFSSPV
                     WAGLDNGDFTSAEAIEQIAQTSSLKKDFIRSLFDLRTEIIFPLTDNIKLLPRLKKSGF
                     KLYYLSNFPLDFFEEVKSEYDFFDYFDGGIISAEVRHSKPDIRIYRILLERHRLKAEE
                     CFYIDDVEINVRAAESAGIRAYCYDSADGNPQHLYELMKEMFPERI"
     gene            complement(9417..9611)
                     /locus_tag="PLZ75_00205"
     CDS             complement(9417..9611)
                     /locus_tag="PLZ75_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HOS70849.1"
                     /translation="MEGTHRSDLKPGLAVLVELNDDKRSGKLTGGRVKEIITTAPIHP
                     HGIKVMLENGLVGRVKQIIC"
     gene            complement(9746..10645)
                     /locus_tag="PLZ75_00210"
     CDS             complement(9746..10645)
                     /locus_tag="PLZ75_00210"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOS70850.1"
                     /translation="MKHPVLFFLLFILSAGISAGQQNGLQTIIKAGARPEKLSDEFSF
                     TEGPAADRSGNVYFTDQPNDRIMIWTTDDRLVTFMQPSGRSNGMFFDPEGNLWSCADE
                     KTALWKISPDKTVEIILNSYNGAMLNGPNDLWTAPGGGIYLTDPFYRRPWWEHKNMPQ
                     EKQRVYYLYPDHKTLVSVEDELQQPNGIIGTKDGKTLYVADIRGNKTWSYTINSDGTL
                     SGKKLFCEMGSDGMTIDNKGNIYLTGKGVTVFDKNGKMLGTIEVPENWTANVCFGGKN
                     RKTLYMTAGKSLYRIPTRKKGVY"
     gene            complement(10709..11611)
                     /locus_tag="PLZ75_00215"
     CDS             complement(10709..11611)
                     /locus_tag="PLZ75_00215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS70851.1"
                     /translation="MSNLSRRKFLSTVAAGTGTILLGPSACSALSGKSDRVSDPFRKI
                     SLGKTGIKTTFLGMGTGYNGYNRSSSITRAGTAESVIRYAWDAGIRYFDCADSYGTHP
                     YTASALRGLPRDKYTIGTKIWVGPGGIPESERPDAGIVVDRFRKELDTDYIDLVQIHC
                     MTDGEWTKRQRRYMEDLEKLKSRNVIRAHGVSVHSLQALRAASEDPWVDVVHVRINPY
                     GENMDDRDPGMVMPLIQKCHDSGKGIIGMKLAGGGKFRNEPEKLDPSLKFVLGLGTVD
                     MIILGFESPEQIDDYIERMNRISI"
     gene            complement(11625..12047)
                     /locus_tag="PLZ75_00220"
     CDS             complement(11625..12047)
                     /locus_tag="PLZ75_00220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOS70852.1"
                     /translation="MKNKVPDMTGYIAELEFIVRDYECDLQGVVNNANYLHYLEHARH
                     ELLISRGISFSLLHEEGTDLIVTKAEIEYKFPLRSRDRFMVRTAVRKEGLIRLAFIQD
                     IFRIPDEKLIVKAKVTGIATREGKPVRFDSIFTLLGLT"
     gene            12226..13509
                     /locus_tag="PLZ75_00225"
     CDS             12226..13509
                     /locus_tag="PLZ75_00225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS70853.1"
                     /translation="MKKENANNGYLFPKRYTNYIFTLLFLLYMFDYIDRMVITSMFPA
                     IERDLGISHTQSGMLVSAVYWAIVLLTFPVSLMVDRWSRTKTIGIMAIIWSLATALCA
                     LTGNYIQLFMARALIGIGEAGYAPGGSAVISGLYPIEKRSRMMGIWNASIPIGTAIGH
                     FLGGFIAVRLGWKHAFGIVALPGLIIAVLFLFMRDYKTVDLAFINKNQQKIRMERKDM
                     VKEFTRRPSVLYTYFGIAAVVFVTTALITWLSTYFQNEQGLPMDTAGAKASVVMVLAV
                     VGAPLGGYLTDAWRKRQPNARLLFPAFSTLTSAVLLFVALYLLRGTVQYIFLLLMGIS
                     IMGFISGAAAVTQDVIHPGLRATSYAIAVVVQNLLGSSMAPMVIGRLYDLYNIKTALL
                     MLPFMLILGSLLFYLGSKHYEKDMKNVAKIRLEAA"
     gene            complement(13519..14493)
                     /locus_tag="PLZ75_00230"
     CDS             complement(13519..14493)
                     /locus_tag="PLZ75_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOS70854.1"
                     /translation="MSILTEIRKPVENEMGKFESYFSRKMKSEIPLLRIILNYILRRK
                     GKQMRPLLVFLTAGLHGRISEATYVAATLIELLHTASLVHDDVVDDANERRGSLSINA
                     LWNSKIAVLVGDYLLSRGLEISLEKNRYDMLEIVSAAVQSMSEGELLQIQKARKMNIR
                     EDDYFRIIRSKTAALLAACTACGAKSVTSDAATISLMKDFGENIGMAFQIKDDLLDYE
                     NNGLTGKITGNDIKERKLTLPLIHALENTSFSTRRHIMSIVRNRKKTLADINEVIDFV
                     KSSDGPEYAAGKMNMYRDRALDILSSYPGSDIRESLRRFVNYTSSREK"
     gene            complement(14684..15292)
                     /locus_tag="PLZ75_00235"
     CDS             complement(14684..15292)
                     /locus_tag="PLZ75_00235"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOS70855.1"
                     /translation="MSAGKKALLLIILILILDQALKVWVKTNMTIGEDIPLLGRIGML
                     HFIENNGMALGMEIGGKTGKLFLSVFRIAAILAIGWFLNSIIRKKASLGLILAVSAIF
                     AGAVGNMIDSAFYGMIFSESFHQPAVLFPPGGGYSSFLHGRVVDMFYFPLINTTWPDW
                     SPFRPGESFIFFRPVFNIADSAITCGVFAILLFQKRMFRDFS"
     gene            complement(15371..16204)
                     /locus_tag="PLZ75_00240"
     CDS             complement(15371..16204)
                     /locus_tag="PLZ75_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70856.1"
                     /translation="MKKITDLLLILILIAGLTSGCSKEKPVPPALPPVESMKIDFSNF
                     ITEKKRASFYDAKGIETASWAFSALAAGYWNSIMVTTLAIPVIAYENAARHSPVYLED
                     NTWEWKYDTRVFMATYRIRITGRTYGHNAEWKMYVSMEGNGSFPEFLWMEGNSRLDGT
                     GGQWTLNHCSFFNEPVMQIDWTSDGTHIQTVKYTYIRELNDSREEDPFRTSFIEFGKK
                     SGSTYDAYFVIHYFNGSGFSDVEVEWSTTEKHGRVRCRAFFADDEWHCWNGNYVNIFC
                     P"
     gene            16454..17080
                     /locus_tag="PLZ75_00245"
     CDS             16454..17080
                     /locus_tag="PLZ75_00245"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOS70857.1"
                     /translation="MKEFKTNEFPALPYSYDALEPYIDARTMEIHYDKHHRTYFNNFI
                     AAIKGSDLEKASIEKIFAEVSKAGDAVRNNGGGFYNHVIFWENLGKGSSGPSPELSAA
                     INAAFGSFEKFKETFSNAAKTRFGSGWAWLYLTSDKKLAVGSTPNQDNPLMDVSPVKG
                     IPLLALDVWEHAYYLKYQNRRPEYVDAFWHVVNWDTVNRRYADATGRK"
     gene            complement(17211..18857)
                     /locus_tag="PLZ75_00250"
     CDS             complement(17211..18857)
                     /locus_tag="PLZ75_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS70858.1"
                     /translation="MGNKETILTFPALFDKTLRNHPEANAYSFVDESPISWTEAGRMI
                     SSVTALLESAGIVPGDRVAILSTNMPNWAVSYFAVTFMGAVAVPILPDFSPVEIINVI
                     EHSGARALFVSSGLKHKTDGLKPGKPETIFSIEDFSVLSPAGSSLKYVPGSLPLRKYD
                     VAGEDLASIIYTSGTTGRSKGVMLTHKNISFNALKGEMIQPVNTNDRFLSVLPLSHTY
                     ENTLGLILPMLGGSCVYYLRKPPTPPVLLPALEKVKPTVMLTVPLIIEKVYYNKILPS
                     LTGKWLMKQLYSIPLIRKRLNRIAGAKLMKTFGGSLKFFGIGGSKLNRTVEKFLLEAR
                     FPYAIGYGLTETAPLLAGANPQKTVFDSTGPAIDEIELKINNPDPKTGEGEIWARGDN
                     VMKGYYREPEMTAEVLTADGWFKTGDLGVFDSRNNLYIKGRLKNMIVGASGENIYPEE
                     IESVINNFRFVLESLVIQQKGKLVAYVHLNMEELERKYRSLKQDLEDRFEEKIQEFIL
                     ELKEYVNTNVNKFSQINKVVLQPVPFEKTATLKIKRFLYI"
     gene            19014..19463
                     /locus_tag="PLZ75_00255"
     CDS             19014..19463
                     /locus_tag="PLZ75_00255"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOS70859.1"
                     /translation="MSIIRVTREFSFEMAHALHGYDGPCKNVHGHSYRLLVTVSGVPV
                     SDGASPKNGMLIDFNILREIVQKKIIDRFDHSLVISNDFDEERKRMMLDAFGNILILN
                     YQPTCEKLVENFAAILGDALPTGVKLHSLRLYETAKSYAEWYASDNE"
     gene            19467..22298
                     /gene="polA"
                     /locus_tag="PLZ75_00260"
     CDS             19467..22298
                     /gene="polA"
                     /locus_tag="PLZ75_00260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOS70860.1"
                     /translation="MHDTDTGKLFLLDAYALIFRSYYAFIRNPRITSKGLNTSAIFGF
                     VLTLEEVLRKQRPSHIAVVFDAPGPTFRHEMYSAYKANRDETPEDIRNAVPYIKELLQ
                     AYKIPVIDFPGYEADDVIGTLAKKAAARGFTIYMMTPDKDFAQLVSDRIFMFKPARSG
                     NESVLWGIEDVRREFSVERPEQVIDILALMGDSSDNIPGAPGVGPKTAMKLIGEYGSV
                     EELLRNTSGLKGRLKEAIEENRDKIELSKKLATIVIDVPVEFREEELISETPDAARLR
                     TLFDELEFRTAGTKILSEIEKAEDTEAVETEKDPAGAVQASLFPASREGAAATETGES
                     GISDRKYTDISGREHDYRLISGMDELREITAMALRQKEFCFDTETSGIDALDSEIVAL
                     ALSWEKNKGYLVWFPPDREETMALLEILRPLFENDNILKTGQNCKFDIHVLGNYGINV
                     KGPLFDTMIAHYLLEPDMRHNMNHLSEVYLSYTPLKIESLIGEKGRQQRSMRSVSPEQ
                     IRDYAVEDADITLQLKNVFKPMIIEEGLDKLAEEIEMPLIRVLADMERKGVLIDENEL
                     GSIATGLREDIINLENEIYDLSGSVFNISSPRQLGDILFLKLRLDDKARLTKTKQFVT
                     SEETLQRLVHKHPVVSKVLEYRGLKKLLSTYVEALPQLINRRTGRIHTSFNQAVASTG
                     RLSSNNPNLQNIPVRDERGREIRRAFVPAEGCLFFSADYSQIELRIMAHLSGDEGMIA
                     DFLSGSDIHAATAAKIFGTGIEDVTREMRSRAKTANFGIIYGISAFGLSERLNIGRKE
                     ARELIDGYFSSYPGVKRYMDESIRKARETGYVTTMFGRRRYLRDILSANQVVRGNAER
                     NAINAPIQGSAADIIKIAMIRIDERLKREAFGSAMILQVHDELIFEVPEDESDRLREM
                     VIHEMSNAAKLAVPLKVDQGSGRNWLEAH"
     gene            complement(22210..23973)
                     /locus_tag="PLZ75_00265"
     CDS             complement(22210..23973)
                     /locus_tag="PLZ75_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOS70861.1"
                     /translation="MKKKISARVAVAISALILGGAGLGFLGIQETRDFRIAKNLDIFL
                     SLFRELNTFYVDEINPDKIIKTGIDNMLKTLDPYTVYYPESEADDFTFMTTGKYGGIG
                     SLVRGGNEYVVISQVYKGFPADMAGIRAGDIIKKVDGVSIRGMSTDKVSDRLKGNPGS
                     EVVVTIERNGRESDHSMKRERIVIPPVPWYGMIDDKTAYIRFTNFTQNCIADVRNALT
                     ELRKSGPEQLILDLRSNPGGLLSEAVEIVNLFVGPGNDVVSTKGKVRQFDDNFRTTKP
                     AADETIPLAVLINRSSASAAEIVAGAIQDLDRGVIVGQRSYGKGLVQVTRPLSYNSQL
                     KVTTAKYYIPSGRCIQALDFSHPDEDGSVGFIPDSLITEFKTRNGRTVKDGGGISPDV
                     AITPEMLSQMSTELYLRNFIFDFATRYYWANPGIKNISDFKFTDKDFEDFGNFLKERR
                     FQYKTITETSLNELIANAKKEKYYELHKDLFSELEKDIAHDLDQDLAIFRDEITQLLE
                     EEIIGRYFYEEGTIIWSVKRDVQIQKALEILNDRAAYGSILSGKSLPVLRANEPPASY
                     VRSPGQLSAERPALQHSTFHV"
     gene            complement(24115..24531)
                     /gene="rnpA"
                     /locus_tag="PLZ75_00270"
     CDS             complement(24115..24531)
                     /gene="rnpA"
                     /locus_tag="PLZ75_00270"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOS70862.1"
                     /translation="MTSTRETFKKAERLSSRKTITMLFENGNTLFTRYFRVIWAESET
                     NSPFPARIAFSVPKKIFRLAVTRNLLKRRIREAYRKNKSHFYKFLVSENIQVALMVIF
                     RKDSVEEYSTLEKAVREMFDKISTEIRGKRNLKRNT"
     gene            complement(24605..25357)
                     /locus_tag="PLZ75_00275"
     CDS             complement(24605..25357)
                     /locus_tag="PLZ75_00275"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOS70863.1"
                     /translation="MKIRKILVSQPEPLNPKSPYFDLAKKYNLKVDFKPFIQVKGVSA
                     KDFRHQKINLNDFSAVIFTSKTGIDHFFRISNEMRVTVPDTMKYFCITENIAFYLQKY
                     IVYRKRKIFHGKARFQDLIDVIGRHRDDNYFVPLPMPHNAEIPELLSKSNIRHTIGIL
                     YKTISSDFTDIKDFDYDILVFYSPSGIKSLKENFPDFVQGDIKIAAFGPTTACAVEQE
                     GLRLDINAPNPKAPSMTMALDNYLKDYTRNCK"
     gene            complement(25403..26425)
                     /locus_tag="PLZ75_00280"
     CDS             complement(25403..26425)
                     /locus_tag="PLZ75_00280"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOS70864.1"
                     /translation="MIIFDRNQNIIFQEPEIQTNNSSPEKIILPAGITEKVNHTLAGD
                     SVAGTARFNSRPENEDSTSAAREGAYPGMLICRSGDLTEAFYSDKSVRFPYSFLKAAE
                     KTNQQNDKVITEHLRDGEIIPPQPFDADWIIFLLIIPAFIYSSLKAFPGKLIGNVKSF
                     ILFKGIGDPSSREKGTFFNWHSGLVNLVSFSSVALFLFLAADYHEFIPFGIQGIGLWL
                     LLFALTAFMVTVRIIVCFILGMISGEKSVFGEYAATIFQSYHITGFILFIISVLLVYT
                     SLFPPAGLLNTGFLLAALVYLMRIFRLLLIFLKQDVSIFYLILYLCALEFLPALIVLR
                     YLTNLF"
     gene            complement(26412..27284)
                     /gene="deoC"
                     /locus_tag="PLZ75_00285"
     CDS             complement(26412..27284)
                     /gene="deoC"
                     /locus_tag="PLZ75_00285"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOS70865.1"
                     /translation="MTKLYEKLIKQCPSETEIKKELKKVSEVMAELPDKYLLMTILSC
                     LDHTSLGTADNKSHILQLTGKINSFSGRFTNIPNVAAICVFPNFVPVVKEKLAVKTVK
                     IATVAGSFPSSQTFRSIKLAECKLALESGADEIDMVISVGSFLNHDFAAVADEIREIK
                     QAMGNKVLKIIIESGLLGDHENIFKASMIAMDAGADFIKTSSGKTSISATPEAAFVMC
                     RAISHFYSETGIRVGFKPAGGITSVNDAVTYYRITEHCLGKEWLNNDYFRIGSSRLAN
                     NILSEIAGERHDHF"
     gene            complement(27403..28749)
                     /locus_tag="PLZ75_00290"
     CDS             complement(27403..28749)
                     /locus_tag="PLZ75_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS70866.1"
                     /translation="MKRSVLLVTAFASFLTPFLGSAINLALPAIGNEFHTTALELGWI
                     VSSFILTSAIFLLPFGRLADIAGRKKIFKYGLSLFSLSTFLIIFSWNTVSLISFRILQ
                     GIAGAMIFGTSMAILTSVFEPGERGKAMGINTTAVYAGLSSGPVIGGLLTQHFGWRSI
                     FLFLVPVGLTSLYLIFTRIKTEWSESKGEKFDWTGSFIYGISLASFMLGFSRLPSAGG
                     FVMIGIALLLGLIFVFFEKNTQNPVFDIRLMLRNRVFAFSGIAALIHYSATSATGFFL
                     SLYLQYLKGFDARTAGLILISQPLMMALLSTFAGKLSDRYNPGIIASYGMALTSSGLI
                     MLCFINGDSPLIFIIILLAVMGIGFGLFASPNSNAIMSSVEKKHLGVASAVLGTMRMA
                     GQMMSMGIAMMLLALYMGQQAIVPSSYPGLMTGMRTGFIIFSILCTIGIFASLARK"
     gene            complement(28791..29243)
                     /gene="dtd"
                     /locus_tag="PLZ75_00295"
     CDS             complement(28791..29243)
                     /gene="dtd"
                     /locus_tag="PLZ75_00295"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOS70867.1"
                     /translation="MRAVIQRVRKSVVSSGEEILASGGPGMMILAGFEEADSAGDVEW
                     LSNKIIQLRIFNDENGIMNLSVRETGGDITVVSQFTLHARTKKGNRPSYIRAASPEKA
                     IPLYEMFVSRLTELLGKPVGTGRFGAMMLVEIHNDGPVTIIIDTRDKE"
     gene            complement(29421..30206)
                     /locus_tag="PLZ75_00300"
     CDS             complement(29421..30206)
                     /locus_tag="PLZ75_00300"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOS70868.1"
                     /translation="MHYRTDYHVHTTFSDGKAAPEDYVSAASGAGLSELGFSEHLSLF
                     GRDQYWVMDPAETGKYLEYIDNLRKTTEKLIIRTGLEVDYVEGKDKEIRDFLDGIELD
                     YRIGAVHYLGEKTVDEGPDFYEGKNIDRLFRSYFSMVMRAVSSEIFDIIAHCDLIRIF
                     GYRPVSSPEPLYRELARTMKRHDVVFEINTNGRNRPVGEFYPDPQFLKVFAEEGVAVC
                     VNSDAHMPSRVGQYFDEAYQLLKSHGFREMATFSRRRRKMMPL"
     gene            30349..31044
                     /locus_tag="PLZ75_00305"
     CDS             30349..31044
                     /locus_tag="PLZ75_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOS70869.1"
                     /translation="MKLNNIDCVCENCDFRDIVHECLDDDEIKGLCRNKKEFSYQKGE
                     VIHHEGEKITSFKYLKSGLVKVFRRTNSGDEQIVTITRPFEFVSNMSVFSGNSYRYSV
                     SALEDSVVCNIDPDYIRDLLMKNGTFSIMLLTRIAEINERIIEQTLDLRQKNLIGRIA
                     YSLLYFSSEIYNSRIFDLPVSRKEIADYIGMSTANVIRTLSDFRKEGIIRIYGKTIEI
                     SDIQKLEILSKRG"
     gene            31075..33078
                     /locus_tag="PLZ75_00310"
     CDS             31075..33078
                     /locus_tag="PLZ75_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOS70870.1"
                     /translation="MKKFIVLTFCVICSSMLSGQPRPDFRGLTDDRSQVQPQGRPGVI
                     SRSPVQSPSRDDPADGGTAWYTLSGMRHGINYFPKVRHRNVQYETGDELLFDNYHSLD
                     VIYDWLGIWAERYSDLVELYEVGRSYEGRPIMQITVTSRKTGKDTDKPAAFFEGGRHS
                     GEVTASETVMWLAKYLLENYGKDPDITHLLDTRTIYLRPVNNPDGHDLYMHTAQSNRS
                     TVRPFDNDGDGLLDEDPPDDLNNDGIIHTMRWKDEKTGNLIPDPGDPSGRIMKRVASG
                     KGIYLSSTEGIDNDRDGRINEDGIGGLDLHRNYPENWRPLTEETGRGFRQGGAGEYPL
                     SETETRSVVAFLLSHPNIFVVNSMDTSVPMHLRAPSTSASEERMYPEDLKWYKYFDEL
                     GKKITGYARAGDVYNDYGGGNPLFGHGPDFGYWYYGAVWYGDEIWNGGRFGDLNGDGV
                     TDQLDMLVWDEKENGGEGFKDWETFSHPDLGEVEIGGFDPKFFQQNPPARHLETWIRN
                     QALFNIEMARHLPELTWENIETKKIRSYKTDSADYQVRVSFRNSGKLPTAFRQADLVK
                     IVTRDRIVLELDTAGTPAGRPAFRILPGAGKRSGSEDRGRYQPEGRMQSPGTVSRNVP
                     FTDGGSVCTEIFNIRLYGNELAGKASVYSTRGGVLKAREFVIR"
     gene            33157..34689
                     /locus_tag="PLZ75_00315"
     CDS             33157..34689
                     /locus_tag="PLZ75_00315"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOS70871.1"
                     /translation="MKTENYSAGDIPRRKFFEMSIKGGLLIASAPALLTRLASCSGSA
                     ENRGEFGMDKPVLDRVIARALGKGGDFADVYLENRISRQIVMEESQFRGGLYGISRGA
                     GVRVISGNKTGYAYTDEITEKKLLHAAEVASYIARGGAGTAPLNVSEAHRESWVTVEV
                     PLQEVTDERRIEMMKRADQAAKDYDPRIRMALIEYNDEIRSRVIANSEGLFLKDELPL
                     LFFIVQVMSDNGTSRHMARERLSFHSGFEMFEKVTPESVAVNASREAIAMLDAGDAPA
                     GIMDVVMQNGWGGVLVHEAVGHPLEADNIARETGAFTGKMGQKVANECFTMIDDGTLP
                     NFRGTINFDDEGTPARRNVLIENGVLKGFMTDILSAKQLSLERTGNGRRESFRHIPIP
                     RMTNTFIAEGGDDPADILASTRKGIYVQSLSGGSVNPVTGIFNFTCREAYLIENGRKT
                     KPIKGATLIGSCMDVISNIDAVGNDLDFGPGICGKGQSAEVTAGQPTVRIRGINVGGS
                     KA"
     gene            34711..>35569
                     /locus_tag="PLZ75_00320"
     CDS             34711..>35569
                     /locus_tag="PLZ75_00320"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOS70872.1"
                     /translation="MNYTDLSEKLLKKCLKAGADAAEVFIQTSRNLSVNILRSEVETI
                     EEASSGGVGFRVFVDGRMGFSHCNDFGSKALEDTIARAVSLARLSSPDEANVLTGDRG
                     ITLVADLYDPMIASESLETKIGMALELEKAAMKDPRITKSAGAGYGENESEIFIANSN
                     GILKSCKSSACSLGVSVVAEKGNQKNTGGEYSTRVFFHDLPDPEKIAAKASRKAIELL
                     DPIMIKTQKAAIIIDPDVARGILGGIITAINGERVLQGASFLRDQMNRQFASSLLTIT
                     DDGTMPGSTG"
BASE COUNT        10049 a         7633 c         8615 g         9272 t
ORIGIN      
        1 tgacggaagc cagagaaagc ttctgggaac agtcaaatac agatcccccg gagaatatct
       61 gatatcgctc agacacaata cgggaattga agctgccaga atattcataa aaggcgattc
      121 agtgatggtt aatgacagaa ttaacagaaa tctgtatatc gcagaaaatg actatctgca
      181 ggatagattc ggaatcggga ccacgcttgt acccctcgtt tttggtgatt ttctggaaac
      241 catgactgac gttgtaacat taaaggactg caggtctggt gtttcggaaa tacagggcta
      301 tgccggaaac cgggatatct ggtattgcat cgactgtaaa agcggcaagg cttcatctgt
      361 aaccgtcagt gaccggatcg gaagaaattc tgtaagcctg agttttggtg aatatagaaa
      421 aacagggtat ttcatttatc caggcagaat aaaaattgag gacaattcgg gactcgggaa
      481 gattagcata tcagtaaatt ctgtggaatt cagggattcg caaaaactgg aattcctgcc
      541 gggaaggaat tacaaaaaaa ttatacttaa gtgaaaaata tagttatctg cctgatgtta
      601 ctggttttga gtaattctcc ggctttggga cagagcaggg ctgatcttga ggatcagaga
      661 aggcggacta ttgaggaaat cggttatgtg gatgaaatgc ttaaatccac cgaaagggaa
      721 cgaaaccagg gtatacaggc cctgcggatt ataggaaaca aggtcagctt gcgggaaacg
      781 gtgctgaaag ggatgaatga ggaaatagag ctgttgaccg gcaggataat tttgaacaag
      841 actgcccttg acatgatgga aagcgatctg cagactctca aggatgatta ttccaggtcc
      901 gtggtaaatt cgtacaaaat ggccaaggca aaccctgatg ttgtctacat cctttcagca
      961 agggatttta accagggata caagagggta aggtatcttc agcaggttac cagatacaga
     1021 aggaatgaat ccgagattat ccgggaactt atgcaggaaa ttaagggagc caaggaaaga
     1081 ctcgagaaag accttgaaag aatatcggaa ctgcaaaaaa gggaaacaca gcagaaagag
     1141 ctgcttcagg ctgaacaacg gaggcagcag aggatggttc agaatttgag taataaggaa
     1201 aaacagctca ggaaggagct ggaagacaaa aagagaattg cccgtaggat tgaggccgaa
     1261 attgctagga ttattgagga agaaaggtta aaagcggcaa aatcgcagct tagcccggaa
     1321 caaaaactta ttggtgataa ttttcaggat aacaggggcc ggttgccctg gcctgttgaa
     1381 agaggcatta ttactgcaca tttcggggtt cacccgcatc ctgtactgaa gtatgtgact
     1441 gaggaaaata agggaatcga gataacaagt aaggggaaag tgatggcacg cagtgttttc
     1501 aagggcgaag tttctgcaat ttctgtaata gcaggtgcaa acattacagt gattataaga
     1561 cacggaaatt atcttacggt gtacagcaat cttataaatg tcagggtcag gaaaggcgat
     1621 aaagttgata ccaggcagga agtcggggag gtatatatcg atcctcaggg agatgataat
     1681 gcggtgctga agtttatgat atttgaagaa aaaacggcac tagatccgga aaagtggata
     1741 atcagtaatt agtagtttta tattattgtt aaaaagagat attttgttca taacatatta
     1801 ataaataaaa attgtgcctt tgaaacataa ttatagtaat ttagtataag gaatattata
     1861 tttccaaaat gcaacagaaa atcaggataa cttcagaaat tgacaaactc agagtagttg
     1921 aaaatgcaat tgacaacctg acaaacgaca atgaaataag tcaggatagt tacggaaaaa
     1981 tactgattgg aactctcgaa gctgttaata atgcgatagt gcacggtaac aagtcggata
     2041 aaagtaaatt tgtcgaggtt gaatttaaat ttgaaaaagg aaatctgaaa gttaccatta
     2101 cagatgaggg tgaaggattc gatccgtcag gcattccgga tcccacactg ccggaaaata
     2161 tcgaagcact cacaggaagg ggtgttttcc tcatgtcaag gctggcagac gaaattaaat
     2221 ttaataacca ggggaacagc gttaccatga cgtttaaagt atgaatgttt tgtgaggata
     2281 agattttttt atgatgaggt aaagtatcgg ttaagagaag ttaaatacct aaaggatttt
     2341 attgctgagg tcatcaggga agaaaagcac agcccgggtg accttagttt tattttaacg
     2401 ggtgataata caatcaggga aataaacagg gagtttctta aacaagatta ctttactgat
     2461 gttatagcat ttgattacgg aaagggaaac aatacctgcg gtgaaatata tataagtgta
     2521 gatacggtta aaaagaatgc cgttaactac aaagttagtt tcaagaatga attaatcagg
     2581 gtaattttgc acggaaccct gcatttatgc ggatacaggg acgggactga agaagatgcc
     2641 cttattatga aggagagaga gaatgaactt ttaaacaggt ttttggaggg aaaaggaagt
     2701 ggaattcaga tatgatgtaa tagttgtggg aggagggcat gccggttgcg aagctgcctg
     2761 tgctgcggca cggcttgggg cgagaactct gctgctaacc atggatctta caaagcttgc
     2821 acaaatgtcc tgcaatccag ctatgggcgg aattgcgaaa ggacagatcg taagggaaat
     2881 agatgccctt ggaggaatga gtgctcttgt tacagaccgc agcatgattc agttcagaat
     2941 gctgaacaaa tccaagggac ctgcgatgtg gagcccgagg gcccagtgcg accgccttct
     3001 tttttcagct gaatggcgga aggtgctgga aaggacggat aatctctttt tatggcagga
     3061 acaggcgctt aaactgatta ttgaaaatgg caggataagg ggtgttgaaa cagaattcgg
     3121 gacaaggttt aaaggagatg ctgttattct gacaacggga acttttctga acggagagat
     3181 gcatgtcggc ctaaggaaag tcaggggtgg cagggctgga gattcggatt cagccgggtt
     3241 gagcgatcag ttggtagatc tggggttcaa ggttgaaagg ttaaaaacag gaaccagtgc
     3301 gaggatagac ggccggacag ttcggtttga aaagatgacc gaacagaagg gggatgagga
     3361 aggcggatcc ttttccttca tgtcggaccg gaaagccata aaggatcaga aaagctgctt
     3421 tattacacat accagcaggg aagttcatga agaactgaga ggcggcctcg aattcagtcc
     3481 tctcttttca ggaaggatca ggggcagggg gccaaggtat tgtccaagca tagaggataa
     3541 aatagtaaca tttgcggata aggattctca ccagcttttc ctcgaaccgg aaggaaggga
     3601 aaccaatgaa tattatctga acggctttgc cagcagcctg ccgctggagc accagtacag
     3661 ggctatgagg aaaatacctg gacttgagga agtggaaatt taccggccgg ggtatgctat
     3721 tgaatatgac ttttttcagc ccacccagct taaacacaca ctggaaacga aaagggtgga
     3781 gaatctttat tttgcaggtc agattaatgg aacaacagga tatgaggaag ccggtgcgca
     3841 gggactgatg gctggcatta atgcggtgtt aaagctcaaa ggccgggata atgttatact
     3901 gggcagggaa gagtcataca ttggtgttct ggttgatgat cttgtgacaa agggagtgga
     3961 tgaaccatac aggatgttta cctcaagggc tgaatacagg attttgctca gacaggacaa
     4021 tgctgatgag aggctgacag aaacaggttt taatatcgga ctggcagaca gggaaaggct
     4081 gaacaggcta aacaggaaaa gagagcttgt aaaagaaata atatggttcc ttgaaaacaa
     4141 aagtgtttca ccggaagaga taaatgagtt cctggcggaa aaatcatcaa gcaaaatcgg
     4201 tcagaaggta aaggctttca gtatagcggc aaggccgcag gttaagttgt ctgaatttct
     4261 gaatctgatc agtcagggag aaaatcttaa agccctccgc tccggaaggt tcgctgaatt
     4321 ttcagaagcg gccgaaatca ggataaaata cgaaggctat atacaaaggg aaaaacttat
     4381 agcagaaaaa ctgaagcgac ttgaaaaaat caaaatccct gcggatataa attacagtga
     4441 actgctctca atttctacgg aggggaggca gaagctggac agaatcagac cagcgtcaat
     4501 tggacaggca ggacgtatca gcggtgtaag tccgtcagac ctgaatattt tattaatgta
     4561 tctgggaagg taaattgttc cacgtggaac aattttaaaa attataattt gaattttatt
     4621 attttgagaa aggaagcagc gtgaaaaaca gcattgatag ctgcgctgta aaagataaag
     4681 cctgtctgtt gacaacaggg ataaggccgt atcttaaaca ggctttttat cagtttcgtg
     4741 aggaggtcat tttgccggag atcactatgc ggggaccggg agattcacgg taaaacaggc
     4801 tggtggctca cagctttccg gaaatttctt aactgaaagg aatactgctc agaccgggaa
     4861 aacggagatg ttgtggaaac ggctgattat ttgttttcac agatcctgta ttttcagttt
     4921 caggaggaag accaggaaaa aggacggtaa ggttcagaag attttaaaaa gaggaaaaat
     4981 gacaatttca ggcagaatcg tggatatcca tcagaggaga atatttccgg gggatgtgaa
     5041 gatagaagga tcgcggataa agagcattgc agaaaggaag gatgcgggag atctgtatat
     5101 aatacccgga ctgattgatg cgcatataca tattgaaagc tcaatgctga cgccggcatc
     5161 atttgccgtg gctgctgttt cacgtggaac aacgggcagc gtgtctgatc cccatgaaat
     5221 tgcaaatatt atggggaggg aaggagttga ttttatgata gaagacggac gaaagacacc
     5281 ttttaaattt tatttcggag ctccctcctg tgtgccggct acggattttg aaacagcagg
     5341 tgcacggctg ggacccgctg aaatccgtga attgctggaa atggaagaga taaaatacct
     5401 tgccgaaatg atgaattacc cgggagttat atatgatgat aaggaggttc tggaaaaaat
     5461 tgcccttgcg aaaagtctga aaaagccggt tgacggtcat gctcccggac ttagcgggga
     5521 cgcactgagg aaatatatcg gagcaggaat tgaaacagac cacgaatgca gcacacttga
     5581 agaggcacag gaaaaaatat ccctgggaat gaaggtaata ataagggagg gaagtgcggg
     5641 cagaaacctc catgccctga aagatctgta ccggacacat ccttccgtga taatgctggg
     5701 cagcgatgat attcatccgg aaatgctgat gaaaaggcat cttgacaagc tgatatccgg
     5761 actcatccgg gaaggataca gccctttcga tgtgatagca agcgcaactc ttaatcccgt
     5821 gatgcattac ggactcgaat ccggactgct gaggccgggc gatttcgcag acctcgcagt
     5881 tgtggatgac ctgcgggaaa tgaatgtact ggaaacctgg ataaacggac aaaaagtatt
     5941 tgaccggggg aaagtccagt tctcagccag gcccggtccg gcggtaaaca ggttcagctg
     6001 ttcggaaata tgcactgagg aaattcttgt aaaaaactgc ggggcaccct tcaggctgat
     6061 cagtgccttt gacggggaat taattacaaa agaggtaatt catgaagcgg tgcagggaaa
     6121 aatggtggaa tcaaatacca ctgcagatgt tttgaaaatt gttgtaaagg accgttacca
     6181 cgattcagct ccggcagttg cctttataag gggttttggt cttaaatcgg gagcattcgc
     6241 ggcatcagtc gcacatgaca gccataatat aattgcaata ggggtgagtg accaggatat
     6301 tgcggatgca gtaaacgaga ttgtcagact gaaaggaggc atggccgttg taaataacgg
     6361 taaaactcat tcaatgcagc tcagcgtggc aggtataatg tcagaccggc cctgtgaaga
     6421 agtggctgct gaatatcagt ttctttccgg actggtcaga gaccttggat gcaaaatgac
     6481 ttccccgttc atgaccctgt cttttatggc actccttgtg attccagaac tgaaactcag
     6541 tgacaggggc ctgtttaaca ccagtaattt cagtttcgta ccgctgtttg ctgaatgaaa
     6601 gcattatcag catccggcac gaaatatccc ggcagaagaa ttcatatctt tgcctgatga
     6661 cattgatacc gcaggatata gtacccttgc ttcctgacaa gcccggagtt taccagtttc
     6721 ttgattcctc cggaagaatt atttatgcag gaaaggccaa aaacctgaaa aaacgggtaa
     6781 gttcctattt ttcaaaaaac cagtcgggga agaccagggt gatgcttgcc agggcagcag
     6841 gccttaacca catagtggtt gacaatgaat cagaagccct gctgctcgaa aacaatataa
     6901 taaagcagca tcagccgaga tataacatac agctcaagga cgacaagact ttcccgtgga
     6961 tatgcatacg gaatgaaccc tttccccgga ttttcagtac aaggaatacc gtaaaggacg
     7021 gctcattata ctacgggccc tatacttcgg cccttatggt taaaaccctg ctggaactca
     7081 tcagaaatat ttacaagatc aggacatgct cacttaacct cagcagggaa aatatagcag
     7141 cgggcaggtt cagggtatgc ctggaatacc acctcggaaa ctgcagggcg ccctgtgtgg
     7201 gactccagga tgaagaggac tattctgaaa atgtacggca gataaaggaa atcctgaagg
     7261 gaaatatatc aacggtgata gatcatctta aaagcctgat gatgggttat gccgcggaat
     7321 tacgctttga agaggcacag gctgtaaaaa ataaaataga tatcctttca aagttcagaa
     7381 gccgttcaac agtggtaagc acaacaataa ggaatgtgga tgtctttgcc atgacacagg
     7441 atacggacag ggcttacatt aattatctga aagtaataaa gggggctgtt atacaggcat
     7501 atacacttga aataagaagc cgtgtagatg aggaaaagga atcgctgctc ggatttgcag
     7561 tcactgaaat aagacaaagg ctgtcaagtg actcccctga aattatagtt ccgttcaaac
     7621 ctgatattct gtttgaaaat attaaatata ctgtcccgaa gaagggcgat aagcagaaac
     7681 tcctcgaact tgccggacga aatgcattgt attacaaact ggaacagcgt aagaagaagg
     7741 aaatcattcc acggggcgac cggacactaa aaaatctgga aaaattacgc aatgatttaa
     7801 acatgagcga aatgccactg catatcgagt gttttgacaa ctcaagtatc atgggacaga
     7861 atccggttgc agcctgtgtg gtcttccgga atggcaggcc cagtaagaag gaataccggc
     7921 atttcaatgt aaaaacagta acaggccagg atgactacag taccatggaa gaaatagtct
     7981 ggaggcgcta caaaagaatt acaggtgaag gaaagcctat gccccagctt attgtaattg
     8041 acggcggcaa gggacagctt tcctccgcga tgaagagcct cagaaaactg aatgtcgggg
     8101 atggcactac tgtcattggg atagcagaga aactggagga aatttatttt cctggggaca
     8161 gtgtgccgat ctatctggac aaaacttcgg taagccttaa aataattcag caggtccgga
     8221 atgaggccca tcgctttgga ataacctttc accggaacaa gcgttcggga gatcttacaa
     8281 aatccagcct tgacagtatt cccggaatag ggatcagaac aaaggagatt ttactgaaga
     8341 aagcaggttc agtggataag atccggaaac tgtccgaaga ggagcttgaa aaactggtcg
     8401 gcaggaaaaa ggcagaaatt ttaaggaatt ttcttaaaga atcgccctga tgcccctccg
     8461 ggaagaagat tcagaaatca tgtttaactg atttgatcag agagcgataa gtgcctcagg
     8521 gtggttactt aactctccgg gaagcctgta ttttaaaact gtccggggaa gagtccggat
     8581 ttttgactgc agcgctgtcc aggaaccagt cttttcaggg aagggatcag gattactgac
     8641 ttcagattct ttcaggaaac atttccttca tcagctcgta cagatgctgt ggattcccat
     8701 cagccgaatc ataacagtag gccctgatgc ctgctgattc ggctgccctg acatttattt
     8761 ccacatcgtc aatgtaaaag cattcttctg ctttaagccg gtgcctctcg agcaggatcc
     8821 tgtaaatcct gatgtcgggt tttgaatgcc tcacctctgc cgaaatgatt ccaccgtcga
     8881 aataatcaaa gaaatcatat tcacttttaa cttcctcaaa aaagtcgagg gggaagttcg
     8941 acaggtaata tagtttaaaa ccgctttttt ttaagcgtgg aaggagtttt atgttgtcag
     9001 tgaggggaaa tatgatttct gtccttaaat cgaaaagaga gcggataaag tcctttttca
     9061 gcgatgatgt ctgagctatc tgttcaattg cttcagcgct ggtgaaatct ccgttgtcca
     9121 gacctgccca gacagggctg ctgaaaatat ccctcataat cagagtgacg gtatcgctgt
     9181 catagcctga ttcttcaaga tactgcccgg gtttccagct cagaaggaca ttcccgagat
     9241 cgaaaacaat attcttaatc atatcagaaa tttttcctga ggagactgag caggccggct
     9301 ccaccgtaaa acaaaaaaaa cacaatgctc cccggccagt gcaataatac tgaatttaat
     9361 ggttgattga ctcttgtgaa ggataaattg tctgaacccg gggaaggttc aggaattcag
     9421 cagataatct gtttaaccct gccaaccagt ccgttttcaa gcatcacctt gattccgtga
     9481 ggatgtatcg gggctgttgt aataatttcc ttgacacgtc cgccagtcag cttgccggat
     9541 cttttgtcat cgttcaactc taccagtacc gccaaaccgg gtttgagatc tgatctgtgg
     9601 gttccttcca tatcaagtaa ttcaaataaa taaacagaat ttatatatta atccagtcgt
     9661 tatacttaac aacaataata gaaaattgaa acaggataaa aaattaaaac cctcctctgt
     9721 tttgacaaac gccggtttta tttgactaat atactccttt tttacgtgta gggattctgt
     9781 acagactttt gcctgctgtc atataaagag tctttctgtt ttttcctccg aaacatacat
     9841 tggctgtcca gttttcggga acttctatag ttccaagcat ttttccgttt ttatcaaaca
     9901 ccgtaactcc ttttccggta agatagatat tgcctttgtt gtcgatggtc attccgtcgg
     9961 atcccatttc gcaaaacaac tttttccccg acagtgtccc gtcgctgttt attgtatatg
    10021 accatgtttt atttcctctt atatccgcaa cataaagggt tttcccgtct ttggttccga
    10081 tgatgccgtt cggctgctga agctcatctt ccacgctgac aagggtttta tgatccgggt
    10141 ataagtaata tactctttgt ttttcctggg gcatattttt atgctcccac caggggcgcc
    10201 tgtaaaaggg atcggtaagg taaatccctc cgccgggtgc tgtccagaga tcattcggac
    10261 cgttcagcat ggctccgtta taggaattaa gaataatttc aacagtttta tcgggactta
    10321 ttttccacag agctgttttt tcatccgcac atgaccagag attgccttca ggatcgaaaa
    10381 acataccgtt agacctgccg gagggctgca tgaatgttac cagcctgtca tctgttgtcc
    10441 atatcattat cctgtcgttg ggctgatcgg taaaatacac gttgccagag cggtccgctg
    10501 ccggtccttc cgtaaaactg aattcatccg aaagcttttc aggcctggcc ccggctttga
    10561 tgatcgtttg aagaccgttc tgctggccgg cggaaatacc tgccgacaga atgaacaaca
    10621 ggaaaaataa tactggatgt ttcatatcag atataattta aattagtctt taacttaaga
    10681 attactcttt cagccgccgc caaatctcct aaatgctgat acggttcatt ctttctatat
    10741 agtcatcaat ctgttccggc gattcaaatc caaggattat catatcaacg gtacccaggc
    10801 cgagtacaaa tttcagggaa ggatccagtt tttccggttc attccggaat ttcccccctc
    10861 ctgcgagctt cattccgata ataccctttc ctgaatcatg gcatttctga atcagcggca
    10921 tcaccatccc gggatcacgg tcatccatgt tctcgccgta ggggtttatc ctgacatgaa
    10981 cgacgtcgac ccagggatct tcggatgcag ctctgagtgc ctgcagtgaa tgaaccgaaa
    11041 caccatgggc ccgtatcaca ttccgggatt tcagtttctc aaggtcttcc atataccttc
    11101 tctggcgctt ggtccattcc ccgtccgtca tacagtggat ctgtaccagg tcgatgtaat
    11161 ccgtgtcaag ctcctttctg aatctgtcga ccactattcc ggcatcaggc ctttcacttt
    11221 cgggaatacc tccgggaccc acccatatct ttgttcctat tgtatatttg tcacggggca
    11281 gtcccctgag cgccgaggcc gtatacggat gagtcccgta actgtcggcg cagtcaaaat
    11341 atcttattcc tgcatcccag gcataacgga taactgattc agccgttccc gccctggtta
    11401 tggacgagga gcggttatat ccgttgtatc cggtacccat tccgaggaaa gtggttttta
    11461 tgcctgtttt tccaaggctt attttccgga agggatcaga aacccggtca gattttcctg
    11521 aaagggcgga acaggcagac ggtcccagca ggattgttcc ggtaccggca gccacggtgc
    11581 ttaaaaattt ccttctgcta agattactca ttttcatttt gtttttaggt taaaccaagc
    11641 aaggtgaata ttgaatcgaa tcgtacgggt ttaccctccc ttgtcgctat tcccgtaact
    11701 ttggccttca caatgagctt ttcatccggt attctgaaga tatcctgaat gaaagccaga
    11761 cggataaggc cttcctttct gacagccgtt ctgaccatga atctgtcacg gctcctgagg
    11821 ggaaacttat attcaatctc tgcttttgtg actatgagat ctgttccctc ttcatgaaga
    11881 agggaaaaac tgattcccct ggatatgagc agttcatgcc tggcatgctc aagataatga
    11941 agataattgg cattgttaac cactccctgc agatcgcatt catagtccct gacaataaat
    12001 tcaagttcag cgatgtatcc ggtcatgtcc gggactttgt ttttcatttt atccaatagt
    12061 ctgaatgata caattgtctg ttacagtgtc cggatgtttt caccggctca tataaattta
    12121 aatttctgac aatatgctaa gcataaagac ttaaaaatac aataaaaaca ttaactgaag
    12181 cattttttta tttatttgga ttttccaaat ttataattgc ctgtaatgaa aaaggaaaac
    12241 gccaataatg gttacctctt ccctaaaagg tataccaatt acattttcac cctcctgttt
    12301 ttgctataca tgtttgatta cattgacaga atggtgataa cgtctatgtt tccggcaata
    12361 gagcgcgacc tgggaatttc ccacacccag agcggaatgc ttgtgtcggc tgtctactgg
    12421 gccattgtcc tgctgacatt tccggtttcc cttatggttg accgctggag cagaaccaaa
    12481 accatcggaa taatggctat tatctggagc ctggccacag ctctgtgcgc acttaccggt
    12541 aactatatac agctgttcat ggccagggcc ctgataggta ttggtgaggc aggctatgcc
    12601 ccgggaggaa gtgcggtcat atctggatta tatcccatag aaaaacggtc ccggatgatg
    12661 ggtatctgga atgcttccat tccgatagga accgcaatag gtcattttct cggaggtttc
    12721 attgccgtaa ggctgggatg gaagcatgct ttcggaatag tcgcactgcc cggactgata
    12781 attgcagttc tttttctttt catgagagat tacaaaactg ttgaccttgc attcattaac
    12841 aaaaaccagc agaagatcag gatggaaagg aaggatatgg taaaggaatt caccagaagg
    12901 ccctcggttc tttatacata ttttggaata gcagcggttg tttttgtaac gacagcactt
    12961 ataacctggt tgtcaacata ttttcagaat gagcagggtc tgcccatgga cactgccggg
    13021 gccaaggcaa gtgtggtaat ggtccttgcc gtggtcgggg ctcccctcgg aggctacctt
    13081 actgatgcct ggaggaagag acagcccaat gccaggctgc tctttccggc tttttcaacc
    13141 ctgacttccg ccgtgttact gtttgtggcc ctgtatctgc tcaggggaac agtccagtat
    13201 atattccttc ttctgatggg aatcagcata atgggattca tttccggtgc ggctgccgtg
    13261 acacaggatg tcatacatcc cggactgagg gccacttcct atgcgattgc agtggtggtc
    13321 cagaacctgc tcggctcctc catggcaccg atggtgattg gaaggctgta tgatctgtac
    13381 aacatcaaaa cggcccttct catgcttcct ttcatgctga tactgggatc cctgctcttt
    13441 tatctgggat cgaagcatta tgagaaggat atgaaaaacg tggcaaagat caggcttgaa
    13501 gccgcctgac agatcttgtc atttttcccg ggagcttgta taattgacga atctcctgag
    13561 agattccctt atatctgacc cgggatagga actgagaata tccagcgccc tgtcgcggta
    13621 catattcatt ttcccggcgg cgtattccgg tccgtccgac gatttcacga aatcgattac
    13681 ttcattgatg tcagccaggg ttttctttct gttcctgaca attgacatta tatgcctcct
    13741 ggttgagaat gaagtgtttt ccagggcgtg gataagagga agggtcagct tgcgctcctt
    13801 tatgtcgttg cctgttattt tcccggtcag tccgttgttt tcataatcaa gcagatcatc
    13861 ctttatctga aatgccattc ctatattttc accgaaatcc ttcatcaggc ttatggttgc
    13921 ggcatcggag gtaaccgatt ttgcaccaca tgccgtgcag gctgcaagca atgccgctgt
    13981 tttgcttctg ataatcctga aataatcgtc ttccctgata ttcatcttcc tggccttctg
    14041 aatctggagc agttcgccct cactcatgga ctggacagcg gcagacacta tctccaacat
    14101 gtcgtaccgg ttcttctcca ggctaatctc cagtcccctt gacagcagat aatcaccgac
    14161 aagcactgca atttttgaat tccacagggc attgatggat aatgacccgc gccgttcatt
    14221 ggcatcatca acaacatcgt catggacgag tgaggcagtg tgcagtagtt caataagggt
    14281 ggctgcaaca taggtggctt cggaaatcct gccatgcagt ccggcagtca ggaagacgag
    14341 aaggggtctc atctgctttc ctttcctgcg aaggatataa ttgagtatga tcctgagaag
    14401 gggtatctca cttttcattt tcctgctgaa gtaggattca aattttccca tctcattttc
    14461 aacaggtttc cttatctcag tgagaattga catcagataa atatttcagc caaacttaca
    14521 taaaatatgc cacaataata cagtttccga aaaatagcct tttcactctg tatgtgatat
    14581 caacatattt gtatatatat ttgtttaaat tattataacg atgaaattca atgaattgaa
    14641 tcctgaaata cttgaaggaa ttatttggcg gtgtcagcag tgctcatgaa aaatcccgga
    14701 acattctttt ctggaacagg agaatggcga aaacaccgca ggtaatggca gaatcagcaa
    14761 tattgaaaac cggtctgaag aagatgaagg attctccggg ccggaaaggc gaccagtcag
    14821 gccaggtggt gtttatgagg ggaaaataga acatgtccac gactcttccg tggagaaatg
    14881 atgaatagcc tccgcccggg ggaaacaata cagccggctg gtggaaactt tcactgaaga
    14941 tcatgccgta aaaggcactg tctatcatat ttccgaccgc acctgcaaaa atggcactta
    15001 ccgccagaat caatcccagg gaggcttttt tcctgattat tgaattaagg aaccagccga
    15061 tggccaggat tgcggctatt ctgaacacgc tcaggaacag cttccccgtt ttaccgccta
    15121 tttccatccc cagggccatg ccgttgtttt caatgaagtg aagcattcct atcctgccca
    15181 aaagaggaat gtcctcacct atggtcatat ttgtcttgac ccagaccttt agtgcctgat
    15241 ccagtataag gatgagaata atcaatagca aagccttttt tcctgctgac attcgagaat
    15301 ttataaaaga ggccggcaaa ataataaaaa aaatcccggt ttgctaccgg gatcctgaaa
    15361 aatgattatt tcaggggcag aatatattta cataattgcc gttccagcaa tgccattcat
    15421 cgtctgcaaa gaaggccctg cacctcactc tgccatgctt ttcagtcgtg ctccattcca
    15481 cttccacgtc ggaaaagccg gaaccgttga aataatggat tacaaaatag gcatcatagg
    15541 tactgccgga tttctttccg aattcaataa aacttgttct gaagggatct tcctcgcgtg
    15601 aatcgttcag ctcccttata taggtatatt ttacggtttg aatgtgggtt ccgtcactgg
    15661 tccagtctat ctgcattaca ggctcattga agaatgagca gtgattgagc gtccactgtc
    15721 ctccggtacc gtcaagcctg gaatttccct ccatccatag aaattccgga aagctgccgt
    15781 ttccttccat actcacatac attttccatt cagcattatg accgtatgtc cgtcccgtga
    15841 tccggatccg gtatgtggcc atgaataccc tggtatcata tttccattcc caggtattgt
    15901 cttcaagata aacggggctg tgccgggctg cattctcgta ggctatcacg ggaatggcaa
    15961 gcgttgtgac cattattgaa ttccagtaac ctgcagcaag cgctgaaaat gcccagctgg
    16021 cagtttctat tccttttgca tcgtagaagg aagctcgctt tttttctgta ataaaattac
    16081 tgaaatcaat tttcatggat tcgaccggag gaagagcagg cggtacaggc ttctccttac
    16141 tgcaaccgct tgtcaatcct gctatcagaa tcagaatcag caacagatca gtaatcttct
    16201 tcatgacggg tatagttatc tgcttccttt taacgcaaat ttaatatgaa agttacatca
    16261 acgccaaaat taataagatt aatggagtat tttaataggt gaactgaaca aaacaacaca
    16321 tattgtgttt aacgtgaaaa ataattttaa aatgaaaaaa tatttttcaa ttgtattatt
    16381 tacaggcttg attctggtat ttacaacggg aatcatcctg gcagagaaac cagtcaacaa
    16441 aaaagatttt aacatgaaag aatttaaaac caacgaattt ccggctctgc cttattcata
    16501 tgatgcgctg gagccttaca tcgatgcacg gacaatggaa atacactacg acaagcatca
    16561 cagaacctat ttcaataatt ttatcgccgc tatcaaggga agcgatctgg aaaaagcttc
    16621 aatagagaaa atatttgccg aagtgtcaaa ggcaggcgat gcagtcagaa acaacggggg
    16681 aggtttctat aaccatgtga ttttctggga aaaccttggg aaaggttcct ccggaccatc
    16741 tcctgaactg agtgcagcta tcaatgcagc ctttggttcc tttgaaaaat tcaaggaaac
    16801 attcagcaat gccgccaaaa cacgtttcgg aagcggctgg gcatggcttt atctgacatc
    16861 cgacaaaaag ctggctgtgg gaagcacacc caatcaggac aatccgctga tggacgtatc
    16921 accggtaaag ggcattccgc tgctggccct tgatgtgtgg gagcatgcat actatctgaa
    16981 ataccagaac agaaggccgg agtatgttga tgcattctgg catgttgtta actgggatac
    17041 ggtgaaccgc agatatgccg atgcaacagg gcggaaataa actactcatg ataccagtta
    17101 aaaaggctgt tccacgttcc ggaacagctt tttttgtttg cccggcatgg gtgatgacta
    17161 taggctgaat gtcccgaacg cgccctgaca gaggggagag cgactgattt tcagatatac
    17221 agaaagcgct ttatcttcag ggttgcggtt ttctcaaatg gaacaggctg aagcaccacc
    17281 ttgttgatct ggctgaactt attcacattg gtgtttacat attccttgag ctcaagaata
    17341 aattcctgaa tcttctcctc gaacctgtcc tccagatcct gcttaagact cctgtatttc
    17401 ctctccagtt cttccatgtt caggtgaaca tatgcaacaa gctttccctt ctgctgtatt
    17461 acaagggatt ccagcacgaa gcggaaatta tttataaccg attctatttc ctcgggataa
    17521 atattttccc cgcttgcacc tacaatcata tttttcagcc gtccctttat gtacaggtta
    17581 ttacgggaat caaacacccc cagatccccg gttttgaacc atccgtcagc tgtaagcacc
    17641 tccgcagtca tttccggttc cctgtaatag cccttcatta cattgtcgcc cctggcccag
    17701 atttcgcctt ctccggtttt cgggtcggga ttgttgattt ttaattctat ttcatctatt
    17761 gcaggacctg ttgaatcaaa aaccgttttt tgcggatttg cacccgcaag caggggagct
    17821 gtttccgtaa ggccatagcc gatagcatag gggaaacgtg cttcaagaag aaacttttcc
    17881 actgtcctgt tgagtttgga tcctccgatt ccgaagaatt taagactgcc tccaaaggtt
    17941 ttcatcagtt ttgccccggc tatcctgttc agccttttcc tgataagggg aatactgtag
    18001 agctgcttca taagccattt tccggtgaga gatggcagta ttttgttgta atagactttt
    18061 tcgattatga gcggtacggt aagcataacg gtaggcttca ccttttcgag agcgggaagc
    18121 agcacggggg gagtcggcgg ctttctgaga taatatacgc aggacccgcc gagcattggc
    18181 agaataagcc cgagtgtatt ctcataggta tgtgaaaggg gaagcacgga aagaaacctg
    18241 tcatttgtgt tcaccggctg aatcatctct cctttcaatg cattgaaact gatattttta
    18301 tgagtcagca tcactccctt ggagcggcct gtggttcccg atgtatagat gattgaggca
    18361 aggtcttccc cggccacatc atatttccgc aggggcaggg aaccgggtac atacttcagg
    18421 gaactgcccg caggggacag aacggaaaaa tcctcaatac tgaatattgt ctccggcttt
    18481 ccgggtttta aaccgtctgt cttatgtttc aggcctgaag atacaaacag ggccctggct
    18541 cccgaatgct caatcacatt gatgatttcc accggtgaaa agtcgggcag aatcggtact
    18601 gccaccgctc ccatgaatgt aacggcgaaa tagctgactg cccagttggg catattggtg
    18661 cttaaaatag ccaccctgtc gccggggacg attcctgcag attcaagcag agctgttacg
    18721 gaagatatca ttcttccggc ctctgtccag cttatcggac tttcatcaac aaatgaatag
    18781 gcatttgctt caggatggtt ccttaaggtt ttgtcgaaga gtgccgggaa tgttaaaatt
    18841 gtttctttat tacccattta agtatgatta aaagtgagta aaggtaatcc aaataacgat
    18901 cccttaagaa aaacatgata aataattcat aagctgcggg gcttcacaat aaaaaagcat
    18961 tcttttcacg gatattgact aattttggca aacggaagat aattcattta ttcatgagca
    19021 ttataagagt tacaagggaa ttcagctttg agatggcaca tgcgctgcat ggttatgacg
    19081 gtccctgtaa aaacgttcac ggccattcat accgtctgct tgtgacagtt tcgggagtgc
    19141 cggtcagtga tggcgcaagt ccgaagaacg gaatgctgat agactttaat attctcaggg
    19201 agattgtaca gaaaaaaata attgaccgtt ttgatcattc acttgttatc agcaatgatt
    19261 ttgatgaaga aaggaaaaga atgatgctgg atgcctttgg gaatatactg attctcaatt
    19321 atcagcccac atgtgaaaaa ctggtggaaa attttgccgc aatacttggg gatgctttac
    19381 ccacaggcgt taaactgcat tcactcaggc tgtatgaaac agcaaaatca tatgctgagt
    19441 ggtatgcgag cgataatgaa taaaccatgc atgacacaga cacaggaaag ttatttctgc
    19501 tcgacgccta cgcgttgatc ttccgttcct actatgcatt tatccgaaat cccagaatta
    19561 cttcgaaggg attaaacaca tctgcaatat tcggttttgt ccttaccctt gaagaggtcc
    19621 tccgcaagca gcggcccagt catatagccg tggtatttga tgctcccgga ccaactttca
    19681 ggcatgagat gtacagcgct tacaaggcca accgtgacga gacaccggaa gacataagaa
    19741 atgcagtgcc ctatataaag gaactgctgc aggcgtacaa aatacccgtt attgactttc
    19801 cgggctatga ggctgatgat gtcattggca ccctggcaaa aaaagctgcc gccaggggat
    19861 ttaccatcta catgatgact cccgacaagg attttgccca gcttgtttcc gacaggatct
    19921 tcatgttcaa gcccgcgcgc agcggaaatg aatcagtgct gtggggaatt gaggatgtaa
    19981 ggagggaatt ttccgttgaa aggccggaac aggttatcga catccttgcc cttatgggag
    20041 attcctcgga caatattccg ggtgctcccg gagtcggccc gaaaactgca atgaaactga
    20101 tcggggaata cggatctgtt gaggaactgc tcagaaacac ttccggtctg aagggcaggc
    20161 taaaggaggc aattgaagag aacagggata aaatagagct ttcaaaaaag ctggccacaa
    20221 tagtcatcga tgtgcccgtt gaattcaggg aagaggaact gataagcgaa acacctgatg
    20281 cagccaggct cagaactctt tttgacgagc tggagttcag gactgcaggt acaaaaattc
    20341 tttctgaaat agagaaggcg gaggatactg aggctgttga aaccgaaaag gatccggcag
    20401 gagccgttca ggcttccctt tttcctgctt cccgggaagg ggctgctgct accgaaaccg
    20461 gagaatccgg aatttcagac cggaaataca ctgatatttc cggccgggaa cacgattaca
    20521 ggcttatcag cggcatggac gaactcaggg aaattactgc tatggcactc agacagaagg
    20581 aattctgttt tgatacagaa acaagcggta ttgatgcgct ggattcggaa atcgtcgccc
    20641 ttgccctttc atgggaaaag aacaagggct atcttgtctg gtttccgccc gaccgggagg
    20701 agaccatggc cctgctggag attctcagac ccctatttga aaatgacaat atccttaaga
    20761 caggacagaa ctgcaaattt gatatccatg ttctcgggaa ttacggcatc aatgtcaaag
    20821 gaccgctctt tgacacgatg atagcccatt atcttctcga acccgacatg cgtcacaata
    20881 tgaaccatct ttcagaagta tatctctcat ataccccgct taagattgaa tcgctcatag
    20941 gcgaaaaggg caggcagcaa aggagtatga gatcagtatc cccggagcag ataagggatt
    21001 atgcagtgga agatgcggat atcacacttc agctcaagaa tgtttttaaa cccatgatca
    21061 ttgaggaggg tcttgataaa ctggcggagg aaattgaaat gcctctgata cgggtacttg
    21121 cggatatgga aaggaagggc gtactgattg atgagaatga gcttggatcc attgccacgg
    21181 gcctgcgtga agatattata aatctcgaaa atgaaattta cgatctttcc ggaagcgttt
    21241 tcaatatctc atcacccaga cagcttgggg atatactttt tctgaagctc aggcttgacg
    21301 acaaagccag gctcacaaag acaaaacagt ttgtaaccag tgaagaaacg cttcagcgcc
    21361 ttgtccacaa gcatcctgtg gtcagcaagg ttttagagta caggggttta aaaaaactgc
    21421 tctcaaccta tgttgaggct cttccgcagc tgataaacag gagaaccgga agaatacaca
    21481 cttctttcaa ccaggctgtt gcatcaacgg gcagactgag ctccaacaat cccaaccttc
    21541 agaatatacc ggtaagggat gaacgcggaa gggaaatacg cagggctttt gttcctgctg
    21601 agggctgcct gttcttttct gccgattatt cacagattga actcaggata atggcacatc
    21661 taagcgggga tgaaggcatg atcgctgatt ttttatcggg cagcgacatt catgcagcca
    21721 ctgctgcaaa aatattcggt accggcatag aggacgtaac aagggaaatg cgcagcaggg
    21781 caaaaaccgc aaacttcggc attatttacg gaatatcggc attcggattg tcggagcggc
    21841 ttaatatagg ccggaaggaa gccagggagc tgatagacgg gtatttttct tcctatccag
    21901 gcgttaagag atacatggac gagagtatac ggaaagccag ggaaacaggc tatgttacaa
    21961 caatgttcgg caggaggaga tatctgcggg atatactgtc agccaaccag gtggtcaggg
    22021 gcaatgccga gaggaatgcc atcaatgccc ccattcaggg ttctgcagcg gatattataa
    22081 agattgccat gatacgcatt gacgaaaggc ttaaaaggga ggctttcggt tccgcaatga
    22141 tattgcaggt tcatgatgaa cttattttcg aagttccgga ggatgaatct gaccgtctcc
    22201 gggaaatggt catacatgaa atgtcgaatg ctgcaaagct ggccgttccg ctgaaagttg
    22261 accaggggag cggacgtaac tggctggagg ctcattagcc ctgagaacgg gaaggctttt
    22321 tccgctcagt atggaaccgt aggctgccct gtcgtttagt atttccaggg ccttctgtat
    22381 ctgtacatcc ctttttactg accatattat ggttccttcc tcatagaaat accggccaat
    22441 gatctcttct tccagcaact gggttatttc atccctgaag atggccagat cctggtccag
    22501 atcatgggca atatcctttt ccagttcact gaacagatcc ttatgaagtt catagtactt
    22561 ttcctttttg gcattggcaa taagctcatt cagagatgtt tctgttattg ttttgtattg
    22621 aaacctcctt tctttcagaa aattgccaaa atcttcaaaa tccttgtccg tgaatttaaa
    22681 atccgaaatg tttttaatac cgggattggc ccagtaatat ctggtggcga aatcaaaaat
    22741 gaaattcctg agatagagct cggttgacat ctggctcagc atttccgggg taatcgccac
    22801 atcaggactg attccccctc cgtcttttac cgttctgccg ttgcgtgttt tgaattcagt
    22861 gatgagtgaa tccggaatga atcccacgct gccgtcttcg tccggatggg aaaaatccag
    22921 tgcctgaatg catcttcccg aaggaatgta atatttggcg gtggttacct tcagctggct
    22981 gttgtagctg aggggacggg tgacctgaac cagtcccttt ccgtaggagc gctggcccac
    23041 aattacgccc ctgtccagat cctggattgc tccggcgaca atttccgcgg ccgaggctga
    23101 cgatctgttg ataagcactg caaggggaat ggtttcatct gcagccggct ttgtggttct
    23161 gaaattgtca tcaaactgtc tgaccttgcc tttggtggaa acaacatcat tgccgggccc
    23221 tacgaacagg tttactattt ccacggcttc cgagagaagt ccgccgggat tgcttctcag
    23281 gtcaaggata agctgctccg gacctgattt cctgagctct gtaagggcat tcctcacatc
    23341 ggcaatgcag ttctgtgtga aatttgtgaa acggatatag gctgttttat catctatcat
    23401 accataccag ggaacaggcg gtatcactat cctttcacgt ttcatggaat gatcggattc
    23461 cctgccattc ctttctatgg tcacaacgac ttctgatccc ggatttccct tcagcctgtc
    23521 actgaccttg tcggtcgaca tgcccctgat ggaaaccccg tcaaccttct tgattatatc
    23581 tcccgccctt attccggcca tatctgcggg aaatcccttg taaacctgac ttataacaac
    23641 atattcattt cctcccctga ccagcgaacc gattccgccg tacttacccg ttgtcatgaa
    23701 ggtgaaatcg tctgcttctg attccggata atatacagta taggggtcaa gggttttcag
    23761 catgttgtca atccccgtct tgatgatctt gtcaggattt atttcatcaa catagaatgt
    23821 attaagttcc ctgaaaagtg acaggaaaat atcaaggttc tttgcaatcc tgaaatcgcg
    23881 ggtttcctga attcccagga aaccaagacc cgcaccgcca aggatcaggg ctgatattgc
    23941 aactgccact ctggcagaaa tcttcttttt catatatttt caacaattga acgcacgaaa
    24001 gtaataagaa taaaatataa accggatcct tcgttgcctg tttgatctga agaacttcct
    24061 gtacagcaaa tccggtgcca ttctcctgtt tcaataccgg cttgacaggg tatttcacgt
    24121 attgcgctta aggttgcgct tcccccttat ctcagtactg attttgtcaa acatttcacg
    24181 tacagctttt tccagcgtac tgtattcttc cactgaatcc ttcctgaata taaccatcaa
    24241 tgctacctga atgttttccg aaacgaggaa tttatagaaa tgggatttgt ttttcctgta
    24301 tgcttccctg attcttcttt ttaaaagatt tcttgtgact gcaagcctga atattttttt
    24361 cggaactgaa aaggctattc ttgcgggaaa gggggaattt gtttctgatt ctgcccagat
    24421 caccctgaaa taccttgtaa aaagagtatt tccattctca aacagcattg tgattgtctt
    24481 gcggctggaa agcctttctg cttttttgaa tgtttcgcgg gtgctagtca tcccggttaa
    24541 gcctgttgta caggccctgt taatttttgc cgttgttctg tctgccgacc ctgaaagcag
    24601 gatcttattt gcaattcctg gtatagtctt tcaggtagtt gtcaagggcc attgtcatgg
    24661 aaggtgcttt aggattgggc gcattgatat caagcctgag tccttcctgt tccacggcac
    24721 aggctgttgt aggaccgaat gccgcaatct tgatgtctcc ctgaacaaaa tcgggaaaat
    24781 tttccttgag cgattttatt cccgaaggac tgtaaaagac tagaatatca tagtcaaaat
    24841 ccttgatgtc agtaaagtca ctgcttatgg ttttatatag tatgcctatt gtatgcctga
    24901 tattgctttt ggaaagcagc tcaggaatct cagcattgtg gggcatggga aggggaacga
    24961 aataattgtc atccctgtgc ctgccgatga catcgatcag atcctgaaac ctcgccttgc
    25021 catggaaaat tttccttttc ctgtaaacta tgtatttctg caggtaaaaa gcaatatttt
    25081 cagttatgca gaaatatttc attgtatcag gaacagtaac tctcatctca ttgcttattc
    25141 tgaaaaaatg atctattcct gtcttgcttg tgaaaattac agcagaaaag tcgttgaggt
    25201 tgattttctg atgtctgaaa tctttggctg atactccttt tacctggatg aacggcttga
    25261 aatctacttt cagattgtat ttttttgcca gatcgaagta tggagatttc ggatttaacg
    25321 gttctggctg tgacactaaa atcttcctta ttttcaactc cttacgattt ttagtttaac
    25381 ttttcggcac tccctggctc gtttaaaaca gatttgtcaa atatcttaaa acaatgagtg
    25441 ccggaagaaa ttccagcgca caaaggtaca aaatcaaata gaaaatcgaa acatcctgtt
    25501 tcaaaaaaat caaaagcagc ctgaaaatgc gcataaggta aaccagggcg gcaagaagga
    25561 atcctgtgtt cagaaggccg gccggcggaa acagactggt atagaccagc aggactgaaa
    25621 ttatgaaaag tatgaagccc gtgatatgat atgactggaa gatggttgcc gcatattcgc
    25681 cgaatactga tttttcaccg ctgatcattc ccaggataaa gcagacgatg atgcggacag
    25741 tgaccatgaa tgcagtcagt gcaaacagta agagccacag cccgattccc tgaatcccga
    25801 atggaatgaa ttcatggtaa tcagctgcaa gaaagagaaa cagtgccact gaagaaaatg
    25861 aaacaaggtt aaccaggcct gaatgccagt tgaaaaaagt tcctttttcc ctggatgaag
    25921 gatctccgat acccttgaaa aggatgaagg attttacatt gccgattaat ttcccgggga
    25981 aggctttcag gctggagtaa ataaatgccg ggattatcag aaggaagatg atccagtcag
    26041 catcaaaggg ctgagggggt atgatttccc cgtccctgag atgttccgtg atcaccttgt
    26101 cgttttgctg attcgtcttt tcagcagctt tcaggaagct gtagggaaag cggacggatt
    26161 tgtcactgta aaaagcttcc gtaaggtccc cgctgcggca gatgagcatg ccgggataag
    26221 cgccttcccg ggcagctgat gtactgtcct cattttcagg ccggctgttg aagcgggctg
    26281 ttccggcaac tgaatctccc gcgagggtat gattcacctt ttccgttatg ccggcgggaa
    26341 ggatgatttt ttcaggagat gaattattgg tctgtatttc cggttcctgg aatataatat
    26401 tctgatttct atcaaaaatg atcatgtctt tctccggcaa tttcagacag aatattgttt
    26461 gccagacggc tggagccgat cctgaaataa tcattattca gccattcctt tcccaggcag
    26521 tgctctgtta tcctgtaata ggtaacggca tcgtttacgc ttgtaattcc gcctgcaggc
    26581 ttgaatccga cccttatgcc cgtttcggag tagaaatggg atattgccct gcacatgaca
    26641 aatgccgctt cgggtgttgc ggatatgctg gtttttccgg aagatgtctt gatgaaatct
    26701 gccccggcat ccattgcaat cattgatgcc ttgaatatat tctcatggtc gcccagcagt
    26761 ccggattcta ttatgatttt cagaaccttg ttacccatgg cctgctttat ttcccttatc
    26821 tcatccgcaa ctgccgcaaa atcgtggttc aggaaggaac cgactgatat taccatgtct
    26881 atttcatcag cacctgattc cagggccagt ttgcattctg ccagttttat ggagcggaag
    26941 gtctgggatg aggggaagga ccctgcaacc gttgctattt ttaccgtttt gactgcaagt
    27001 ttttccttta ctacgggtac aaagttcggg aaaacacaaa tagccgcaac gtttggaata
    27061 ttcgtaaacc tgccggaaaa actgttgatt ttgcctgtaa gctgcaatat atggctttta
    27121 ttgtcggcag tgcctagact ggtatgatca aggcagctta atattgtcat cagcaaatat
    27181 ttgtcaggga gctctgccat gacttccgac acttttttca gttctttctt tatctctgtt
    27241 tcagaagggc actgtttgat cagtttctca tacaatttgg tcatcgcatc aggttaaagt
    27301 aaatgaatat atatctatgt cagacggggc gtaatttaat gctttttata tataaaaata
    27361 tttttttaac aaatttatcc ccggaagttc cggtttatac agttatttcc ttgcaaggga
    27421 ggcgaatata ccaatagtgc agagaatgga aaaaattatg aatccggtac gcattcctgt
    27481 catcagtccg ggataggacg agggtacaat tgcctgctgt cccatgtaca gggcaagaag
    27541 catcattgct attcccatgc tcatcatctg gcctgccatt ctcatggttc ccaggactgc
    27601 cgaagccacg cccagatgtt ttttttccac cgaactcatg atggcattcg aattgggtga
    27661 ggcaaacagg ccgaaaccta tgcccattac cgcgagaagg atgattataa atatcagggg
    27721 actgtccccg tttatgaaac aaagcatgat aagtcccgaa gaagtcaggg ccattccata
    27781 cgatgcgatt attcccggat tatacctgtc ggacagtttc ccggcaaaag tagacagcag
    27841 ggccatcatc aggggctggg atatcaggat cagtccggca gttctggcat cgaagccctt
    27901 gaggtactga aggtaaagac tgagaaagaa tcctgtggcc gaagtggcgg aataatggat
    27961 cagggctgca atacctgaaa aagcaaaaac cctgtttctg agcatcagcc ttatatcaaa
    28021 aaccgggttt tgcgtattct tctcgaaaaa aacgaatatc agtcccagca gaagggcaat
    28081 tcctatcatg acgaagcctc cggctgaggg cagccgcgaa aatccaagca taaaggaagc
    28141 aagcgaaata ccatagatga aggaccctgt ccagtcaaat ttttcgccct ttgattcgga
    28201 ccattctgtt tttatcctgg taaatatcag atacaaagaa gttaatccca ccggaaccag
    28261 gaagagaaat atgcttctcc agccgaaatg ctgtgtaagc agtcccccga tcaccggccc
    28321 gcttgagagt ccggcatata ctgctgtggt atttattccc atggcctttc ccctttcccc
    28381 gggttcgaaa accgaggtca ggattgccat gctggtaccg aatatcattg ctcccgctat
    28441 accctgcaga atcctgaagc tgatcagtga aaccgtgttc caggagaata ttatcagaaa
    28501 agtggacagg gagaaaaggg acaaaccata tttgaaaatc ttctttctcc cggcaatatc
    28561 ggcaagtctg ccaaagggga gaaggaaaat tgcggatgta agaatgaagc tgcttactat
    28621 ccatccgagc tcaagcgcag tggtatggaa ctcatttccg atggctggaa gggcaaggtt
    28681 aattgcagag cccaggaatg gggtcagaaa ggatgcaaaa gccgtaacaa gcagcacaga
    28741 tcttttcatg ctcgtttcaa cccggaaaga ctgctctttg ttcatcggaa tcactcttta
    28801 tccctggtgt caattattat tgttaccgga ccgtcattgt gtatttcaac cagcatcatg
    28861 gccccgaacc tgccggttcc gacaggtttc ccgagaagtt ccgtgagcct tgacacgaac
    28921 atttcataaa ggggtatggc tttctcaggg gaggcagccc tgatgtatga aggcctgttt
    28981 ccctttttcg tcctggcatg aagggtaaac tggcttacca ctgttatgtc acctcccgtt
    29041 tcacggacgg aaagattcat aataccattt tcatcgttga agatcctgag ctgtattatc
    29101 ttgttgctca gccattccac atccccggca ctgtctgctt cctcaaagcc ggcaagaatc
    29161 atcatcccgg ggcctcctga tgccagaatt tcctcaccgc tgctgacgac cgatttcctt
    29221 acacgctgaa tgactgccct catatttccc gccttgcttt aaacttaaaa cctgcaaata
    29281 tacatgatca gattgacaag ctcctgattg ttttccggaa atttaaccgg gcagctgttt
    29341 ccgttcacgg ggaagaggaa ctgatatacc gcagggggat taatgacaat tccggtgaag
    29401 gctgacatgt tccggcaggt tcacaggggc atcatttttc tccgccgcct gctgaaagtt
    29461 gccatttccc tgaaaccatg tgatttcagc agctgatatg cttcatcaaa gtactgaccc
    29521 accctggaag gcatgtgtgc atcagaattg acacatacgg caaccccttc ctcagcaaac
    29581 accttcagaa actgcggatc gggataaaat tcacccacag gcctgttcct tccgttggta
    29641 ttgatctcaa aaaccacatc atgccttttc attgtcctgg caagttccct gtacaggggt
    29701 tccgggctgg atacgggcct gtaaccgaaa atcctgataa ggtcgcaatg ggcgataatg
    29761 tcaaaaattt ctgatgacac tgcacgcatg accatggaaa aatagcttct gaaaagccgg
    29821 tcaatgtttt ttccttcata aaaatccggt ccttcatcca cggtcttttc accgagataa
    29881 tgaaccgctc ctatacggta gtcaagttca atgccgtcaa gaaaatccct tatttcctta
    29941 tcctttcctt ccacatagtc aacttccaga ccggttctga taatcagctt ctctgtagtt
    30001 ttacgcagat tgtcaatata ttcaagatat tttccggttt ctgccggatc catcacccag
    30061 tactgatccc ttccgaaaag ggagaggtgt tccgagaagc ccagctccga taaaccggca
    30121 ccggatgccg ctgaaacata gtcctcagga gctgctttcc cgtcgctgaa ggtggtgtgt
    30181 acatgatagt ctgtcctgta atgcatagtc ggatcttgtt ttataaaccg ggaaaaagtg
    30241 atcaaagtag tcctaaattt gttaataaag caaacagccg gcgtaaatta taccggctta
    30301 aggtaataat gatgaggaaa aatgtaaatt tgcagaggag caataaatat gaaattgaat
    30361 aacatagatt gtgtttgtga aaactgtgat ttcagggaca tcgttcatga atgtctcgat
    30421 gatgatgaaa taaaaggact gtgcaggaac aagaaagaat tttcatatca gaagggagag
    30481 gtgattcacc atgaggggga aaagattaca agtttcaaat atctgaagag cggactggtt
    30541 aaagtgttcc ggagaacaaa ttcaggcgac gagcagatag tgacaatcac aaggcccttt
    30601 gaatttgtga gcaacatgag cgttttttca ggaaacagct accgttattc ggtttcggca
    30661 ctggaagatt cggtagtatg caatatagat cctgattata taagggacct gctgatgaag
    30721 aacggcactt tctcaataat gctgctgaca agaatcgctg agatcaatga aaggatcata
    30781 gaacagaccc ttgatctgag acagaaaaac ctgataggac gcattgcata ttccctgctc
    30841 tatttcagca gtgaaatcta caattcgaga atcttcgacc tgccggtttc aagaaaggaa
    30901 attgccgact atatcgggat gagcactgcc aacgtgatca gaaccctttc tgatttcaga
    30961 aaagaaggaa ttatcaggat ttatggcaaa acaatagaga tatccgacat tcaaaagctt
    31021 gaaatactct caaaaagagg ataacagaac cctttaatat ctgaattgct gattatgaag
    31081 aaatttatag tactgacctt ttgtgtcatc tgctcatcca tgctttccgg ccagccccgg
    31141 cctgatttcc gcggactgac ggatgaccgg agccaggttc agccgcaggg ccggccgggc
    31201 gtcatatccc gatctcccgt tcagtcccct tcccgggatg atccggcaga tggaggaact
    31261 gcctggtaca ctctcagcgg aatgcggcac gggataaatt attttccgaa ggtacgtcac
    31321 cggaatgttc agtatgaaac gggggatgaa cttcttttcg acaattatca ttccctggat
    31381 gtcatttacg actggctcgg gatatgggct gaacgttaca gtgatcttgt ggaactgtat
    31441 gaagtgggaa ggagctatga ggggaggccc ataatgcaga ttaccgtcac cagcaggaaa
    31501 accgggaagg atactgacaa gccggctgca tttttcgagg ggggaaggca cagcggcgaa
    31561 gtaacagctt cggaaacggt gatgtggctt gcaaaatacc tgctggaaaa ttacggaaag
    31621 gatccggaca ttacccatct tctggataca aggacaatat acctgcgccc ggtgaacaat
    31681 cccgacggtc atgacctcta tatgcacacg gcacagagca acaggagtac cgtaaggcct
    31741 ttcgacaacg acggggacgg cctgctggat gaggatccgc cggatgatct gaacaatgac
    31801 ggcattattc atacaatgcg gtggaaggat gaaaagaccg gaaacctgat acctgatccg
    31861 ggagatcctt ccggaagaat tatgaagagg gtggcctcgg ggaaaggtat ttatctttca
    31921 tcaacagaag gcattgacaa tgaccgggac gggcgtataa acgaggacgg gataggagga
    31981 cttgaccttc accggaacta tcctgaaaac tggaggccgc ttaccgagga aaccggtcgc
    32041 ggattcagac agggcggtgc aggggaatat ccactgagcg aaacggaaac caggtcggtt
    32101 gtggcttttc tcctgtcgca tccgaacatt tttgtggtca attcaatgga taccagtgtc
    32161 ccgatgcatc tcagggcccc gtcaacatcc gcttcggaag agcgcatgta tccggaggat
    32221 ctgaaatggt ataagtattt tgatgaactc ggtaaaaaga tcaccggtta tgccagggca
    32281 ggagatgtgt acaacgacta tgggggaggg aatccccttt tcggtcatgg tcccgatttc
    32341 ggatactggt attacggcgc cgtgtggtac ggtgatgaaa tatggaacgg cggcaggttc
    32401 ggggatctga acggcgacgg ggtgaccgac cagctggaca tgcttgtctg ggatgaaaag
    32461 gaaaacggcg gggaagggtt caaggactgg gaaacattca gtcatcccga tctgggtgag
    32521 gttgagatcg ggggctttga tcccaagttc ttccagcaga atcctcccgc gcggcatctg
    32581 gagacatgga tcagaaacca ggcactcttc aatattgaaa tggccaggca ccttccggaa
    32641 ctgacctggg aaaacatcga aacaaaaaaa atcagatcat ataaaacgga ttctgccgat
    32701 taccaggtca gggttagttt cagaaacagc ggcaaacttc ccactgcctt caggcaggct
    32761 gatctggtga agattgtcac ccgcgacagg attgtcctgg aactggatac cgcgggaacc
    32821 cctgccggca gaccagcctt caggatactt cccggtgcag gcaaaaggtc cggcagtgaa
    32881 gaccggggca ggtatcagcc tgagggccgg atgcagtcgc cgggaactgt ttccaggaat
    32941 gttccattca ccgacggagg atcggtgtgc acagaaatat tcaacataag actctacggg
    33001 aatgaactcg caggaaaggc aagcgtgtat tctacaagag gcggtgttct gaaagccagg
    33061 gagttcgtaa tccggtaatg tgatcagagt aataattgac ggttaaagca gccggatcag
    33121 gtacagctga taaagattcc agaaaaacaa gcatatatga aaactgaaaa ttacagtgcc
    33181 ggggatattc ccaggcggaa attttttgaa atgagcatca agggcggcct gctgatagca
    33241 tctgcaccgg cacttcttac ccggctcgct tcatgcagcg gctctgcgga aaacaggggg
    33301 gaattcggca tggacaaacc ggtccttgac agggtaatag ccagggccct gggcaaaggc
    33361 ggtgattttg ccgatgttta tctcgaaaac cgtatttcga ggcagatagt aatggaggaa
    33421 tcacagttcc gcggcggact ctatggcata agcagggggg ccggcgtgag ggtgatatcc
    33481 ggaaataaaa caggatatgc ctatactgat gagataacgg agaaaaaact gctgcatgcc
    33541 gccgaagtag cttcctatat tgccagggga ggggccggaa ccgctcccct gaatgtttcg
    33601 gaagcacacc gggaatcctg ggttactgtg gaggtgcctc tgcaggaagt tactgatgag
    33661 cggaggattg aaatgatgaa aagggctgac caggcggcaa aggattatga tccgagaatc
    33721 aggatggccc tgattgaata taacgatgaa attagaagca gggtcattgc caacagtgaa
    33781 gggctttttc tgaaggatga gctgcccctt cttttcttca tagtacaggt gatgagcgat
    33841 aacggaacaa gccgccatat ggcacgggaa aggctcagct tccactccgg ttttgaaatg
    33901 tttgaaaagg tgaccccgga atcggtggca gtgaatgcat caagggaggc aatcgccatg
    33961 cttgatgcag gagatgcgcc ggccggaata atggatgtgg tcatgcaaaa cggctggggg
    34021 ggcgttcttg ttcatgaagc tgtgggacat ccgcttgaag ccgacaatat tgcaagggaa
    34081 acaggtgcct ttaccggaaa gatgggacaa aaagtggcta acgagtgctt tacaatgatt
    34141 gatgacggca ctctgcccaa cttccgcggt actataaatt ttgatgatga aggtaccccg
    34201 gcccgtagaa atgttctgat agaaaatggt gtcctgaagg gattcatgac cgatattctg
    34261 agtgcaaaac agttatcgtt ggaaaggacc ggcaacgggc gcagggaatc attcaggcat
    34321 ataccgattc cgcgcatgac aaacaccttt attgcagaag gcggggatga tcctgctgat
    34381 attcttgcat caacacggaa gggcatctat gtacagagcc tcagtggcgg aagcgtcaat
    34441 cccgtaacgg gtatcttcaa ttttacctgc agggaggcct atctgataga gaacgggaga
    34501 aaaacaaaac ccataaaggg agcaacgctt ataggatcgt gtatggacgt catttcaaac
    34561 atagatgctg ttgggaacga tctggacttc ggtccgggaa tatgcggcaa ggggcagtct
    34621 gcagaagtta ctgccggcca gccgacagta aggataagag ggatcaacgt gggcggaagc
    34681 aaagcataac aataaactga agaaagaaaa atgaactaca cggatttatc ggaaaagctg
    34741 ctgaaaaaat gcctgaaggc cggtgcggat gccgctgagg tattcattca gacgagcagg
    34801 aatctttcag taaacatact caggtcggaa gttgaaacca ttgaagaggc ttcatccggc
    34861 ggagtgggat tcagagtgtt cgttgacggc agaatgggat tcagccattg caacgacttc
    34921 ggttcaaaag ctcttgagga tactattgcc cgggccgtat cacttgcccg cctgtcctcg
    34981 cctgacgaag ctaatgttct cacaggcgac aggggaatta ccctggttgc ggatctgtat
    35041 gaccccatga ttgcctctga gtccttggaa acaaagatag ggatggccct ggagctggaa
    35101 aaggcagcga tgaaggatcc caggattaca aaaagtgcag gggcgggtta cggcgaaaac
    35161 gaatcagaaa tatttatcgc taattccaac gggatactga aaagctgcaa atcgtcggca
    35221 tgttcgctgg gtgtatccgt ggtggcggaa aaggggaacc agaagaatac cgggggagaa
    35281 tacagtaccc gggtcttttt tcatgacctg cccgatccgg aaaagatagc agccaaagcc
    35341 tcaaggaaag caattgaatt actcgacccg ataatgataa agacccagaa agctgcaatc
    35401 attatcgatc cggatgtggc caggggaatc cttgggggaa tcatcaccgc cataaacggc
    35461 gagcgtgtac ttcagggagc cagctttctc agggaccaaa tgaacaggca gtttgcatct
    35521 tcccttctta caataacgga tgacgggacc atgcctggca gcacgggca
//
LOCUS       DAOSPX010000003        34239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_1398_length_34239_cov_4.800170, whole genome shotgun sequence.
ACCESSION   DAOSPX010000003 DAOSPX010000000
VERSION     DAOSPX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1398_length_34239_cov_4.800170"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1681)
                     /locus_tag="PLZ75_00325"
     CDS             complement(122..1681)
                     /locus_tag="PLZ75_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS70873.1"
                     /translation="MKRAEQTALLKQEKDFLWDITVIGGGATGLGIAVDAATRGYKTI
                     LLEKSDFAKGTSSRSTKLVHGGVRYLAQGDVLLVTEALRERGRMLKNAPHLTGNQEFI
                     IPAYSWWDVILYTAGLKFYDLLSGRLSLGKSYFINPAKTLKRLPLLNPEGLKGGIVYH
                     DGQFDDSRMAVEMARHCTVNGAGVINYFEVRELLKDESGRITGVRGMDTVTGGIYSIR
                     SRLVINATGVFTDEIHRMDDPEARPTVRPSQGVHIVLDRSFLPEDSAIMIPRTDDGRV
                     LFAIPWYDRVVAGTTDTPLDIVTNEPKALDEEIDFILRTAGKYLVKKPRRSDVLCIFA
                     GLRPLAADPDNPASTKEVSRRHKITLSSSGLLSITGGKWTSYRRMAEETINRGIRAGI
                     LEKRKCVTRNYSFCPSGVKLRSERLKIYGSRADVIERMIEENPDLGELLDPRLPYTRA
                     EIVWICHNEMPVNLEDVMARRTRSLILDARASSDIAPEVAAIMARELKHGDDWVQNQI
                     KEYNKLLINYL"
     gene            complement(1741..2835)
                     /locus_tag="PLZ75_00330"
     CDS             complement(1741..2835)
                     /locus_tag="PLZ75_00330"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOS70874.1"
                     /translation="MLSFLAMIGFTTSGCGENPKFFAGGYAKGEEKGMFVYEMDKKNG
                     SVRLLSQADAGPNPSYFCYSPENKLVYAANEVGMFLGQRGGGLTTLRYNDQDATLEKI
                     SEMVVPFGGPCYISMSPDRGYLFMANYGSGSVAVVRLNGEGIPETVTDTVRYMVERPA
                     RSNPHMVMSDPLGDHIFVTDLGQDRFVVYEFDAGTGRLILAEDGMVNVEKGSGPRHFA
                     FSADASRMYLINELGSTMMTFKNDHKGNLSHMQTVSTRGFNSAEKNYPGDVHAGKNGE
                     YIYGSNRGENSIVIYKVAGDGTLDLAGHTTCGGDWPRNFVIDPSGEFLLVGNQRSDNI
                     AVFKINKRTGMPEGPVENVVMQAPACLKFF"
     gene            3450..6578
                     /locus_tag="PLZ75_00335"
     CDS             3450..6578
                     /locus_tag="PLZ75_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS70875.1"
                     /translation="MKKFNKINQMVFPGDEIKPSLKSIFIAALLFIFGAIAIPGLYAS
                     DDGAPVQQQQVSGRITDAVTGQAMPGVNIQVKGTTLGSISDIEGRFTLSVPDLNSTLV
                     FSFIGYISQEVPLAGRTSLDIALGEDLTQLEEVVVVGYGTVKKATITGAISTVEGVQL
                     QKSPAVNITNSFAGRLPGLMVVTRSGEPGNDQSILRIRGSNTLGDNRPLIVIDGIANR
                     SMDRISSSDIESITVLKDASAAIYGAQAANGVILITTKRGMEGKPRITVNLNQGWSMP
                     TVLPKMADAATYAEMINEINYYDGLPPKYSPEEIQKYRDGSDPWNYPNTDWYGETMKK
                     ASGQNFANLDLRGGSAAVKYYVSLGGNFQDGIYKNSATNYKQANFRSNIDATITRHIK
                     LSLDVAGRQETRNYPTRSASGIFSMLMRGYPVAHAYWPNGLPGPDIALGNNPVVITTS
                     MTGYDKQKDYVMESVIKLDVSIPWVKGLSFASNISFDKNIRNKKLWETPWYLYVWDGV
                     SLEADGTPELSKALRGLPSPQLTQSMIDGNRTTLNALVTYARTFSEAHNFKLLVGSER
                     ISGESMNFEAFRKYFVSNVVDQLFAGSELEKTNNGSASLNARLNYFGRFNYDYIGKYL
                     LEFVFRYDGSYIFPADKRFGFFPGVSLGWKISEENFWKDNLSFINTFKLRGSWGQTGN
                     DRIETYQYLSSFGFSGSRYVFQNEEKILNELRIPNPDVTWEVANQLDLGFDGAVLNGN
                     LEFQFDYFHNLRTNILWYRNASVPTTSGLTLPRENIGEVVNRGFDFQVTYRDVIGQLN
                     YTLSLNSGYQKNKIRFWDETPGIPEYQKSTGRPMNAGLYYKAIGIFKDDEALNGYPHW
                     QGARPGDIIFEDINKDGKIDGLDRYREPKTDLPVFSGGFNFDLTWKNLYSSVFLQWAT
                     GAVRNNYYEMQGEAGNFLAEDAAGRWTEQNPNASKPRTWNRYYGYWREQSNTYWLRST
                     DYMRLKNIEVGYNIASLPSVKNLGIESLRVFFTGLNLLTLDKLKDFDPESTSSTSYPL
                     NKVYNIGVSLTF"
     gene            6589..8391
                     /locus_tag="PLZ75_00340"
     CDS             6589..8391
                     /locus_tag="PLZ75_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS70876.1"
                     /translation="MKSFKFIYLLLPFLFVLGNSCSEDFLETKPLTEISEVDVWGDPA
                     LARLFVNHIYFNTPEPFRRGRLSSNLVDEADYRGNTASLNFNNGIITMDATPTWNNIY
                     YSRTWADLYSNIRYCNSFLENADKIPFDDNTEKNKMVGEVHFLRANLYHELVAMYGGV
                     PLVKSTYRLQDDFEIPRDSYEDCIKFIVDECNIAAGMLPESHSGADVGRATKGAAMAL
                     KSRVLLYAASDLHNTNMFSSFSEPKLLQYTGGDRKARWQAAKNAARDIIDLNLYRLNN
                     ANPSPSDSIAKDYADYFITLSYTPEDIWAKFVVTESGQMIGLYSLMNGYHGWGTNCPV
                     GDMVDAYEMRDGTRFDWNNPAHAASPYKNRDQRFYATVFYEGAKWRARPGDVAEKDPL
                     NAVQVGTWEKWDEATQSVNYVFGLDTRKGGIEEWNGSYTGYYMRKFIDPAWDVSFIES
                     GGGGYQTVPYRFFRYAEILLNYAEACIELEEYDEARTYINMVRRRAGQPDLTESGSAL
                     RERYRNERRIELAYEEHRIHDVRRWAIGPQAYKPVTAAKVVYKLLPDKTTATVPTITH
                     EVFQTRKWLDKAYFMPIMRDELNKNSALVQNPGY"
     gene            8523..9698
                     /locus_tag="PLZ75_00345"
     CDS             8523..9698
                     /locus_tag="PLZ75_00345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 domain-containing metallophosphoesterase
                     family protein"
                     /protein_id="HOS70877.1"
                     /translation="MKKLLIILLLLPAYCQTLPAQEQAFKITHGPYLTDMSETAVTIV
                     WITNKNALAWVEVAPDDGSHFYGKERPRYYDTYLGRRQAATTLHRVRIENLTPGTRYR
                     YGIFSKEVLHWENDARIVYGTTIANPAYSRNSLSFKTFSSSDDTVSFIMLNDIHGRAD
                     FMKELCRDIDFNTIDLVVFNGDMSSAIHSEEQIFSDFMDAAVDLFAKRVPIAFTRGNH
                     ETRGPYADYLMYYFPLKGEKIYRTFNVGNVAFIMLDCGEDKPDSDIEYSGLADFDAYR
                     NEQAAWLRQAVLEKSFRDTRNKIVILHMPPGVSDWHGTLHIDEVLVPLLNEAGTDAVL
                     SGHTHRYSFHPPVQGKTAFPVVVNSNNTYLRGDVVNGKIRIRMTGTAGTKPIEHILE"
     gene            9788..10693
                     /locus_tag="PLZ75_00350"
     CDS             9788..10693
                     /locus_tag="PLZ75_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS70878.1"
                     /translation="MIKSLSRTRIFFLSFVLLTCPLYLLDAQKAGDSGQPGSFSFVYV
                     TDIHLDYNTTTLQYFDQAVNTINELDPDFIITGGDNIKDATQPRRSYADSLFNLYLSQ
                     IKRFRMPVYTGIGNHECFGINNPDISDEDPLFGKKMYESRIGKRYYTFTHKGWKFFMI
                     DDIRITDTGHKYIGHIDDEQMEWLKNELSATDFLTPVVVCSHIPFISSMKKFEFGSLA
                     GNPDNDGVSNSIEFFRLFEHHNLKLLLQGHFHFFEVLYTNNIYYVTGPSLNGRYGSML
                     TKTAGYILFTTDGDRLSWQLIKNEL"
     gene            complement(10821..11756)
                     /locus_tag="PLZ75_00355"
     CDS             complement(10821..11756)
                     /locus_tag="PLZ75_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS70879.1"
                     /translation="MINSRRNFIKKAAVSGAAVLAAPSAFGLGKQRLPDATAPFKLKY
                     GPSIGMFRHHAGTDPVDNIKFCYDQGFRAMFDNGLMNRPVEEQLRIAGEMNRLGMEMG
                     PFVLYADFSVTSFVLNKEEVREMLSKKMQEGVECFKRTGVKWALVVPGRYDERLHRDY
                     QTANVIDNLRMCCDIVEPAGLTLVLEPLNTLINHPGLFLTGIPQAYSICRAVNRKSCK
                     IVNDLYHQQITEGNLIPNMGAAWSEIAAFHIGDNPGRNEPTTGEINYRNVFKYIYERK
                     FDGVLGMEHGISQRGTKEGEARVIQAYRECDNFEI"
     gene            complement(11800..13191)
                     /locus_tag="PLZ75_00360"
     CDS             complement(11800..13191)
                     /locus_tag="PLZ75_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS70880.1"
                     /translation="MKKSGISRRGFLGTTVAGAAGIVAPVIITGCSSGKDKPFKPVPD
                     GTWLDAAPDGPVLKAGVIGCGGRGSGAAINFLNAGPNLQVTALGDTFGDRVEQAKSAI
                     LKQKGQEVPEENCFVGFDAFEKVIASDVDIVILAEPPYFRPAHLAAAVAARKHIFAEK
                     PVCVDPTGARSVMATALKAQELGLTIVTGTQRRHQRNYVANYNQVKAGAIGDIVGGNI
                     WWNGGKLWHRDSNPAWSEMEWMIRDWVNWTWLSGDHIVEQHVHNIDVMNWFTGSHPVK
                     AHGMGSRLRRVTGDQYDNFSIEFIFEDGRRFHSQCRQINGCWNSVAERLQGTKGSTNC
                     ADTIHDLAGNEIWKYQYPLDSEGNPSSRSMMDPYVQEHIDMVTAIRNNQPFNELENTA
                     ISTMVGIMGRISAYTGRETTWEEMMNSDLKLGPKVFAFGKVDIPKEVPIAGEAYDAGA
                     EAKRAAARAARSS"
     gene            complement(13373..14863)
                     /locus_tag="PLZ75_00365"
     CDS             complement(13373..14863)
                     /locus_tag="PLZ75_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS70881.1"
                     /translation="MKTSRRDFLKSTAIAGAGAIAGNMIQSCAPREAQSNLPEIIAAA
                     RKKHSMHFNMSGYAAPALPVVRTGFVGLGDRGSGAVQRLSNIEGVEIAAFGDLREAAV
                     KGSQRYLERIGRPAAQEFFGDEDSWKKLIELPGLDLIYICTPWDMHAAMANYAMECGK
                     HVVCEVPLVKTVDEAWMLVETSEKTRKHCMMLENCCYDFFEMLTLNMARNGLFGDIIH
                     GEGAYIHNLLGMNFKKPIDDRQSDGAYTGMWRLKENASRNGILYPTHGLGPIAQVLNI
                     NCGDRMDYLTSMSTNDFTMHQREEELVAGDSFYEPWKNSRFRGNFNTTLIRTALGKTI
                     KVQHDVSSEQPYSRIHLVKGTTGMAVKYPVERIAFGESWVKDEEVAELRDKYTPEIIK
                     RVGEMARRVGGHGGMDFIMDWRMIDCLRNGLPLDQNVYDGAAWSVVAPLSEWSVANRS
                     QTIDIPDFTCGAWKTNKPHDINLEAGGTTQVLEVRRRQESLQLNVV"
     gene            15165..16649
                     /locus_tag="PLZ75_00370"
     CDS             15165..16649
                     /locus_tag="PLZ75_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biosynthesis protein"
                     /protein_id="HOS70882.1"
                     /translation="MPESLKNITCLFFFVALIPCIPGCRTESGGDLPESDRFPRISPD
                     YTDISIPYNIAPLNFVVKEKGSAYFVRISSENDTAIRLKSRKAAIRIPQGKWKKLLSA
                     NRGKQLNIEIYAKQKKYGWIKYKTVTNVISSEAVDPFVTYRLILPGYERWSDISIRQR
                     DIRGFREWPLIKNSVADRNCVNCHSFNNGRTDDFLFHMRGSRAGTYFYSEGKLIKINL
                     KTPEMKNGPVYPRWHPSGRFVAFSANNIVQQFHSANGNKVEVSDLESSLLLYDTGKNE
                     IMPVDLPDRGKYMDTYPEWSPDGRMMYFCRTSRLEENYDYRHTRYDLYRVSFDPSSRR
                     FGEPELVINASEKAKSISFPRISPDGRFLAMTYHDYGCFPVWHREADLILLNLETLEI
                     INPELNSDFPESYHSWSSNGRWLLFASRRDDGLATRPYLAYVGQDGKSGKAFIMPRRN
                     PEHYNTFLKSYNLPEFSNLKITMNPGRIRNVVTSGALQAEWGGK"
     gene            16656..18422
                     /locus_tag="PLZ75_00375"
     CDS             16656..18422
                     /locus_tag="PLZ75_00375"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOS70883.1"
                     /translation="MKIKSAKLTGFFSVLPSRLFFLFAFLYFYFFGNHVLFFQEKTSL
                     FIFSFEFLTENIKQPGGFLVYISAFLATFSCYPAAGALIYATILWMIVVFSLEITVAV
                     SGSKRVLIPYLAGLALFYLQLDYRYLLFNNTGILLQILFFWLSVRYLKGFIPVLLLPL
                     FYFLTGGFTLIYLFMFSAWLLYTRPEAWKLKMLSFWLIILLFFGLSKEFLFFQSADTL
                     LKYPFTEADTGSQIYIFSAAAFMIAFLPLLPAFRKKKPVRTEIFRLPASLTGSAILFI
                     LLSSIAALRYDKKTSQYFRVESLFYEGRYQDVIDYNIRHPSGNILTLFLNNIALCETG
                     RLNDMLFHFRQSPDGGTLFQKWDISSEISRRGGYFYYTIGMMNEARRWAFENMVTRGF
                     TPEGLRLLIKADIINGDYHTAAKYIRILKKTLFYRDEARQFEKLLFDDKAVDMHPELG
                     ERRRSKARTDFITISGIPLINIERMLATGSVNRKAFEYKLAWLLLTKDHQGILQEWDK
                     LGEYGFEKVPVHMDEAAAAVKVLSAGQFIPQGDFTVSPDTELRFERFLQTFRAYGNNP
                     KAAEPALRKQFGNTFWYWNFYH"
     gene            complement(18572..20455)
                     /locus_tag="PLZ75_00380"
     CDS             complement(18572..20455)
                     /locus_tag="PLZ75_00380"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS70884.1"
                     /translation="MKKLLSYISAISVLLFLLASCTDVLDQDPVSSFNEQVVFADVNV
                     TKAYLGKCYDRMGGNTDNGILGMREDLLSSATDQTLCIHRPANYVNLKGTMSPDQLGY
                     FANTGYAGFLRWPFIYQNIQNVNTILANIDNVPAPTSTEEALKIQIKGEAYFIRAFEY
                     ALVLMNYGGAVIATEPFQLGGDFLSKKRSSLQETLDQVLSDIDKAIANLPETMQQGRA
                     TRAAAAALKSRVLLFCASDLVNGGYEAGKTLVSFGTGGQAARWIAARDAAKAVMDGNY
                     GTFSLVGNISNPPPGGYSEAEVQAIAETYFGIFNQKGAWNSETIWGIQYPLTGGNINR
                     ANIWFGPCGYHNWGNNNPTEPAVRQYEMADGTPFEWDRYDPGNQVLRKATAAQLAAEP
                     QRNPYAGREARFYGTVLYHGAPWQASPADRAAFDPTDRVQTGHLYNMDGTVKSYGVDT
                     RQGQIEAWNGTKNGYYLKKFMDPATVGQYYYNTNTWVEFRYSEILLNYAEACIEIGGG
                     DLQNGLDALNMVRNRAGLPDRVTTDQAEARKWVRHERYLEHFAEGHQWYDMRRWMTAP
                     DVIGNVYEMKVREFVNGDWEWEYDLSAKPDDRSWANNKFYWIPLARSEINKAPQLEQA
                     PGY"
     gene            complement(20484..23681)
                     /locus_tag="PLZ75_00385"
     CDS             complement(20484..23681)
                     /locus_tag="PLZ75_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS70885.1"
                     /translation="MKKTISSFWPDGPELNFHSLSLTLKILTFLLVMGMVMPAWSLSP
                     DHIPADMQQQVRVSGTVTDAATGQAMPGVNIVVRGTTLGTISDSDGRYEIAGVNANAT
                     LVFSFIGFISQEVPVSGRSVVNVELTAEITDLDEVVVVGYSTQKRANVIGSVTAISGQ
                     SIQAVPAPTVSTSLGGRLPGSVVIQNNGEPGQYGTRILVRGRSTLGGGRNPSSAASET
                     APLVIIDGVPGRNMDEVDPNDIESLSVLKDASAAIYGAQAANGVILITTKKGTEGKTR
                     LNYQFYQGFQTPSVIPQVCNSAEYATMLSEYQVATGKSRTYTDKDIELFRSGADPWRH
                     PDTDWYADLIKKWTTTYRHNLTMDGGTKGMLYYISVGLKGDESFYKQSSTKYSQYNVR
                     AKVDMPITDWFKTGVELAGFQINRVYPYKSADAIVGQSTRLVPTNWSFWPNGLPGPDI
                     EYGDNPVVTSTFQGGKNDQKTYRLLSSFNTTLTIPFVEGLSLNGIFSYDLTNYYQKAF
                     YKPWVLYYPNWDEAVIDPHTGFVTDMPMKPTLRGLSSPQNTERFDRTINKTINLGVTY
                     TRKFYDHNIFVYGGFEQFTNDYNWFRGFRELYISSLIQTMNAGADLNKNTDGEASIYA
                     RKSYIGRVTYDFRGKYLAEALFRADGSLKFPPSSRWGYFPGFMAGWRASEEGFWRDNI
                     PFINYFKLRASWGKMGMDPGTAFQYMNSFGLGGGMSFGTSAAIESVVGPPTIANPNIT
                     WETQTTRNVGFESKWLNDLIHWNFEFFKNKREDILAPRDASVPDFTGLSLPNENIARV
                     DNSGFEMEAGVHKNLTADLRLDVSGNLSYNHNKVVFKDEPERAVPWQRETGHPYNAWL
                     MYKCIGVFADDAAVKNYPAWTGAKPGDLIFEDVSGDGKIDGNDRILIDEVDVPKTYYG
                     FNIDASWKGLSLSLMFQGQGKFYKFNHYDERRGEAGNYFKWTYDNRWTPDNRNTNIGR
                     AFNRNDFYWAHAVNMSTYWLDNTAYCRLKNLVITYNIPRQIFRGIGISNANVYFSGNN
                     LALLWTATRKFDPEANGAGVYPIMKTFAFGANISF"
     gene            complement(23914..25446)
                     /locus_tag="PLZ75_00390"
     CDS             complement(23914..25446)
                     /locus_tag="PLZ75_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS70886.1"
                     /translation="MKKFLIWFTPGLVLGILLVLGSGLMIEKTSTNEYCMSCHIHPGA
                     DNSWKKSVHYVTKSGYRVGCTECHLPPKGEGYLWAKATTGLRDLWSYWTKDSASFDWD
                     AKGRLEHARLHTYESSCIKCHENLFPPELTKEGEDAHLYYRQTKKTPDLHCINCHLNS
                     GHHIEGYTTGSNVTFGAVSSAPKELFTTAAKVSAFESFTEQIPNSSVSFNMIAIPGGR
                     FRMGSPAGEPFRRSNEGPVREVELSPFFMAEVEVTWDEYLAFYAQTSAEGRTTDTEGI
                     RSGQAEETDAISGATPPYGQPDQGWGMGQRPAISFTHHAAETYCRWLSQVTGKTYRLP
                     TEAEWEYACRAGTETPYFFPGDPDNFEKTGFRAKFSKNDTAVINSYVIFQGNSPSKTQ
                     TPDMVKPNPFGLKNMLGNVAEFCSDWYQEDAYSEYPADEIILDPQGPESGEEHVIRGG
                     SFQDMAGKVRSAARGHTQTDEWLRTDPQIPKSIWWYSDCYHVGFRVVCEFDENTGNIA
                     SR"
     gene            25586..27022
                     /locus_tag="PLZ75_00395"
     CDS             25586..27022
                     /locus_tag="PLZ75_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOS70887.1"
                     /translation="MERILSILLALLITAVVDCHPWKPNHYVIIDTDGGIDDIRAITM
                     FLASPDVRILGITTSGGALSPGNSYIKVKSLLNSMFHAGIPVGSDTGGSYGMKEMPFA
                     LQARWGSEDGISPKEAPDNLDIISSLLKAEKTKISFVCLGGMQTALKAFRNIPEFRTQ
                     VKDIVWSVNESGYRNGFNYRIDREAADAMLKQEIPVRMVRNMPSGQNDFWNDGLIRDL
                     AGIKNPYAAKLSTFFANEDFRSHRFSFSGSDEMAAVFIHYPSLFVNKITGSISESTPA
                     DIEGIREGTLKIISMKTIEENQVIKELPLDPAFYFDDLSPVVEEIIEKHGVEEWKSGI
                     FASEMHRHLGIFEIIGVKMGIRAREYFNTGVDEFRAVSYAGSVQPMSCMNDGFLVSTG
                     STPGHGLLTVRNDTALIPMVDFIYLGRKYRIKLKSEITTKISSELKEINFIYGLDSNI
                     YWELVRKNTIKYWRDMDRHDIFEIGELN"
     gene            complement(27143..28753)
                     /locus_tag="PLZ75_00400"
     CDS             complement(27143..28753)
                     /locus_tag="PLZ75_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOS70888.1"
                     /translation="MAKFIYEKPFQIEKDETEYRLLTKDYVKTTEAGGMKILQLDPRG
                     LEVLAAVAISDLSFFLRTSHTRKLRDIIDDPETSDNDRFVAYTMLRNSVISAKEELPW
                     CQDTGTAIVIAKKGEQVWTAADDAMHLSKGIFKAYQEKNLRYSQIVPYTMFEEKNSCT
                     NLPAQIDICSVEGNEYEFLFITKGGGSANKTFLYQESKSLLNDESLTRFVREKIQDLG
                     TSACPPYHLALVIGGTSAEETLKTVKLATAGWLDHLPVTGSEGGRAFRVPKWEERIQK
                     ICREYGVGAQFGGKYFVHDVRVIRLPRHAASCPAGLGVSCSADRNLKARITEEGIFLE
                     ELEKNPARFMPDKAPGLQEPVEIDLDRPMKEILAQLSRHPVKTRLSLTGTLIVARDLA
                     HARIKQMLDEGKPMPEYLKNHPVYYAGPAKTPPGMVSGSFGPTTAGRMDAYVDLFQSL
                     GGSLVMLAKGNRSRAVIESCRKHGGFFLGSVGGPAAVLAAENIKSVEVVDFEELGMEA
                     VRKIRVENMAAFIITDDKGNDFFDEYNK"
     gene            complement(28796..29635)
                     /locus_tag="PLZ75_00405"
     CDS             complement(28796..29635)
                     /locus_tag="PLZ75_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS70889.1"
                     /translation="MKVHLFNIANFKVDGGAMFGVVPKVLWSRVYESDENNLITLSLR
                     SLIVSTGDRVILVDTGWGDKQDEKFFRHVHLHNGDGLTEGLLKHGYSPGDVTDVFLTH
                     LHADHCGGCVRKNAKSTGYELVFPNAVYHVSRIQWEWALRNNPREEDAFLEENYIPVY
                     DSGRLNLIDEEGELFKDFSVRICYGHTPGLMIALVNYRGKTLVYAGDLIPTAAHLPLI
                     WNMSYDIESLKTISEKKKILEEALENDYILVIQHDRHVECCDLHMTSRGIRAKNTFKL
                     SDI"
     gene            complement(29699..30907)
                     /locus_tag="PLZ75_00410"
     CDS             complement(29699..30907)
                     /locus_tag="PLZ75_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOS70890.1"
                     /translation="MKKPSGLSGYKGGHYVELLRGGEPFFAAVEKIIDEARHFIHFQT
                     YILDEDETGTRMVKALIRAAERGVRTYLLLDAYGTKYLSKDFIDSIENSGILFRFFSP
                     TFTGKGFQLNLRLHSKVVLADGEAAIIGGMNFANRYHGSPRKKEWLDFAVLFRGPQCV
                     HTLSILKRLWNKTFISKEERSQETVDTHLFYREDIRLKVLQNNWYRNKMEISKSYREI
                     FRLAGSRIIIFNSYFLPAGNERKLMRNAGLRGVDISVVFSAESDAPVFKKATEFLYDF
                     ILKSRIRIYEYLPSNLHAKVATVDGMWCTVGSYNMNHLSDYASIEINVGILDDKFTKN
                     FEKELTEIIRNDCREITLEEYSRRRRWYSQFAGWLSYQVLRLLMRITYQMTTKKSKPR
                     ISYTASDITR"
     gene            31196..31675
                     /locus_tag="PLZ75_00415"
     CDS             31196..31675
                     /locus_tag="PLZ75_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70891.1"
                     /translation="MKKTFLLLAFTALIGLQVHGQDYKTSAGLRAGVPFGLTVKHFLT
                     KTNAVEGILASTPWGGFVITGLYENEHWTGKYPGLNWFWGAGAHVGFWDRGRNNPYFS
                     DSYRGNAVLGLDAIIGLEYTFDDIPLNLSLDLFPTFNIFGYTGWGGINGGISVRYVF"
     gene            31867..31938
                     /locus_tag="PLZ75_00420"
     tRNA            31867..31938
                     /locus_tag="PLZ75_00420"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31900..31902,aa:Glu,seq:ttc)
     gene            32187..33233
                     /locus_tag="PLZ75_00425"
     CDS             32187..33233
                     /locus_tag="PLZ75_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70892.1"
                     /translation="MKRQKQSNTVVSIKDEKFLINGRPTYQGISWQGLPIEGLLLNSR
                     MVQGIFDDKNPVTAKRWKYPDTQKWDPDRNTDEFVKAMDEWYARGLISFTINLQGGSP
                     VGYGRIDCINSSIKPDGELDNKYMGRIEKILNRANEKGMIVILGIFYFGQDQNIKDEA
                     AVKKGVENTVDWLFEKNYRNVIIEINNECNIKDYDHDILKPERVHELISFAKSKIKNG
                     YRYLVGTSYGGGTVPLENVVAVSDFILIHGNGVEDPAGITEMVKETRAVKGYKPMPII
                     FNEDDHFDFDKPVNNFTEAIKVYASWGYFDYRMKGEGFDDGFQSLPVNWSISSQRKKA
                     FFDKLEEITGGFKK"
BASE COUNT         9112 a         8258 c         7525 g         9344 t
ORIGIN      
        1 aatactgtgt gaattctttc tgtgccgttg caagaggcaa tccgttatga tcaaagacta
       61 tagccctgga acttgtcgtt ccctgatcaa gtgctaaaat gaatttttcc atcaggaaat
      121 attacaaata attaattaat aatttattat actcttttat ctgattctga acccaatcat
      181 cgccatgttt cagctcacgc gccattatcg cggcaacctc aggtgcaatg tctgaacttg
      241 cccttgcatc cagaatcagg gaacgggttc ttcttgccat tacatcttca agattcacgg
      301 gcatctcatt atggcatatc cagacaatct cggccctggt atagggtaaa cggggatcca
      361 gcaactcacc cagatcagga ttttcctcaa tcattctttc tatcacatca gccctgctcc
      421 cgtagatttt cagcctttca gacctgagtt tcacacctga tggacagaaa ctgtagttac
      481 gggttacaca ttttcttttt tccaggattc ctgcccgtat ccccctgttt attgtctctt
      541 cggccatccg tctgtatgag gtccacttcc cgccggtaat ggacagcagt cccgagctcg
      601 agagggtaat cttatgtctg cgagacacct ccttcgtgga tgccgggttg tccggatctg
      661 ctgccagtgg gcgtaatccg gcaaaaatgc agagaacatc actgcgccgg ggttttttta
      721 caagatattt tcctgcggtt ctcaggataa aatcaatctc ttcatcaagg gctttgggtt
      781 cattggtgac aatatcaagg ggtgtgtcgg ttgttccggc aacaaccctg tcataccagg
      841 gaatggcaaa aaggaccctg ccgtcatcgg ttctgggtat cattattgct gaatcttccg
      901 gcagaaagga cctgtccagt acaatatgca caccctggct cggcctgacg gtaggtcttg
      961 cctccggatc atccatacgg tgtatctcat cggtaaagac accggtcgca ttgataacca
     1021 gcctcgatct gatgctgtaa atcccgccgg taacagtatc catccccctg acaccggtga
     1081 tcctgccgct ttcatccttc agaagttccc ttacctcaaa ataattaatt acgcctgctc
     1141 cgttcactgt acaatgccgg gccatttcaa ccgccattct tgaatcatca aactgcccgt
     1201 cgtgatatac aatacccccc ttcagacctt cgggattcag caggggaagg cgcttcaggg
     1261 tttttgcagg attgatgaaa tatgatttcc cgagactgag ccttcccgac agaagatcat
     1321 aaaatttcag tcctgcagta tacagtatca cgtcccacca tgagtatgcg ggaattataa
     1381 attcctggtt cccggtaaga tgaggtgcat ttttcagcat tcgccccctt tcccggaggg
     1441 cttcagtcac cagcagcaca tcaccctgtg caagatatct tactcctcca tgaaccagtt
     1501 tggtgctgcg tgaggaggta cccttcgcga aatcggattt ctcgagaaga attgtcttat
     1561 agcctcgtgt tgctgcatca acagctattc cgaggccggt agccccgccc ccgatcaccg
     1621 tgatatccca taaaaaatct ttttcctgct ttaataaagc agtctgctct gcgcgtttca
     1681 tcaggaaaga actaaaatcc cggaaaaact atttatatct ccccggccga aatgtgaacc
     1741 ctaaaagaat ttcagacagg ccggtgcctg catcacaaca ttttctacag gaccttcagg
     1801 cattcctgtt cttttgttga tcttaaacac tgcaatattg tcggacctct gatttccgac
     1861 aagaaggaat tctcccgaag ggtctatgac aaaattcctg ggccagtctc caccacaggt
     1921 agtatgtcct gccagatcga gtgtcccgtc accggctacc ttgtaaatta ctatcgaatt
     1981 ctcacccctg ttcgatccgt aaatatattc cccgttcttt ccggcgtgaa catcaccggg
     2041 ataattcttc tcggcggaat tgaaacccct cgtggaaacc gtctgcatat gggacagatt
     2101 acccttatga tcgtttttga atgtcatcat cgtggatccc agctcattga tgagatacat
     2161 cctgcttgcg tcggcactga aggcaaagtg tcgcggaccc gaaccctttt caacgtttac
     2221 catgccatct tcagccagga tcagcctccc ggttcctgca tcaaattcat ataccacaaa
     2281 gcggtcctgc cccaggtcag ttacaaatat atggtctccg aggggatcgc tcataaccat
     2341 gtgcggattc gacctggccg gcctttccac catatacctt actgtgtccg tcacagtttc
     2401 cgggatccct tcaccgttca gcctgaccac ggcaaccgag ccgcttccgt aattcgccat
     2461 aaagagatat cccctgtcgg gtgacatgga aatataacag ggccctccga agggaacaac
     2521 catttcactt attttttcca gagtggcatc ctggtcattg tatctcaggg ttgtcagacc
     2581 acccccgcgc tgcccaagaa acattcctac ctcatttgcc gcatacacga gtttattttc
     2641 cggtgagtaa caaaaatatg agggattggg acctgcatct gcctgcgaca agagcctgac
     2701 cgacccgttc ttcttatcca tttcataaac aaacattccc ttttcttcac cctttgcata
     2761 accacctgca aagaatttcg gattctcacc acaaccggag gttgtaaatc caatcatcgc
     2821 caaaaatgat aacattaata atcctgttct tttcattctc attttatttt gtgaaactgt
     2881 ttctgatatt tcaatgcaag cccaaccgaa catgcctggc gtgaacagta aaggatcctg
     2941 ttactaagcg cttaatgttc agaagcctca aatatacttt aaataaaaat cataatttgt
     3001 gaaaataacc gattaaattt ttattttcac ccgaaaaatc tgttaaaaat gatcaattat
     3061 tcacctgttt ccgttattcc gttaactctt attttgttat tgcctgattt gacgggagcg
     3121 gactgcactc atttcaggtc tgcacttttg aaaacaggga ttatggatac tattgatgcc
     3181 cttagggagg aaaggatgat ttcctgtttt ccgccggggg cctgcctctt gttaccgcgc
     3241 cgtaatgccg gaagaacaat aagtaagact gaataatact aatcctaaag ctttttacca
     3301 acctaaatcc caagcctatg aaacaagcca aattctgatc cggcacatgt tgttgtgcct
     3361 ccggtttatc tgtttgtcat caacacatta ttcagggact gttttccctg aagggagtgt
     3421 atagtatttc aattaaaaat cattcaaaca tgaagaaatt caataaaata aatcaaatgg
     3481 tattcccggg ggatgaaata aaaccatccc tgaagagtat ctttattgca gctcttcttt
     3541 ttattttcgg agctattgcc attcccgggc tttatgcctc tgacgatggt gctcctgtcc
     3601 agcaacagca ggtcagcggc aggataacgg atgccgtcac tggtcaggcc atgcccggtg
     3661 tgaacattca ggttaagggt accacactcg gatccatatc ggatattgaa ggcagattta
     3721 cgctcagcgt ccccgacctg aactcaaccc tggttttctc ctttataggc tatataagcc
     3781 aggaagtgcc tcttgccggc aggacctcgc tggacatagc cctcggagag gaccttacac
     3841 aacttgagga ggtagtggtt gtgggctatg gaacggtcaa gaaagcaacc attacaggtg
     3901 caataagcac ggtggaaggt gtgcagctgc aaaaatcccc ggctgtaaac ataacaaact
     3961 catttgcggg aagacttccc ggactgatgg ttgtcacacg ctccggcgaa cccgggaatg
     4021 accagtcaat attgcggatc aggggatcca acacccttgg tgacaaccgc ccccttattg
     4081 tcatcgacgg aattgccaac cgcagtatgg accgtatcag ctcttccgat atcgaatcca
     4141 tcactgtact gaaggatgcc tctgccgcaa tttatggcgc acaggcagcc aacggggtta
     4201 tactgataac aaccaaacgc ggtatggaag gaaagcccag gattacagtc aatctcaacc
     4261 agggctggag catgcccacg gtactgccca aaatggcaga tgcagccaca tatgcagaaa
     4321 tgataaatga aatcaactac tatgatggtc ttcctccgaa atattctccc gaggaaatac
     4381 aaaaatacag ggacggctct gatccgtgga actatccgaa tactgactgg tatggtgaaa
     4441 ccatgaaaaa ggcatcaggc cagaactttg ccaatcttga cctgagagga ggatctgcag
     4501 ccgtcaaata ttatgtgtcg cttggcggta atttccagga cgggatatat aaaaacagtg
     4561 cgacaaacta caagcaggct aacttcagaa gcaatattga cgcaaccatt acccggcata
     4621 tcaagctcag ccttgatgtt gcaggccgcc aggaaaccag gaactatccc acacgttcgg
     4681 cctccggaat cttcagcatg cttatgaggg gatatcccgt tgcccatgca tactggccca
     4741 acggattgcc gggacctgac atagccctgg gaaataaccc cgtggtcata acaaccagca
     4801 tgaccgggta tgacaagcaa aaggattatg taatggaaag cgttatcaag cttgatgttt
     4861 ccattccatg ggtgaagggt ttgtcatttg ccagcaatat ctcgtttgat aaaaacatac
     4921 ggaacaagaa actctgggaa acaccctggt acctttacgt atgggacgga gtatcacttg
     4981 aagctgacgg cacaccggaa ctttcaaaag cactcagggg actcccctct ccgcagctga
     5041 cacaaagcat gatcgacgga aacagaacaa ctctcaatgc actggtaacc tatgcaagaa
     5101 ctttttccga agcccataac ttcaagttac tggtgggctc tgaaagaatt tcgggagaat
     5161 cgatgaattt tgaagcattc agaaaatatt tcgtatcgaa tgtggttgac cagctgttcg
     5221 caggaagtga actggaaaaa accaataacg gctcagccag cctgaatgca cgtctgaact
     5281 atttcggccg attcaattat gattacatcg gaaaatacct cctggaattt gtcttcaggt
     5341 atgatggttc atatatcttc cccgccgaca aacggttcgg attcttcccc ggagtatccc
     5401 tgggatggaa aatatcggaa gagaacttct ggaaagacaa cctgtcgttt attaatacct
     5461 ttaaactcag aggttcatgg ggccagaccg gtaacgacag gattgaaacc tatcagtatc
     5521 tctccagctt tggcttctct ggcagcagat acgttttcca gaatgaggag aaaatcctga
     5581 atgaacttcg tatccccaac ccggacgtca catgggaagt agccaaccag cttgatctgg
     5641 gttttgacgg tgcggtactg aacggcaacc tggagttcca gtttgactat ttccataatc
     5701 tccgcaccaa tatcctgtgg tacaggaatg cttccgtccc cacaacaagc ggattaaccc
     5761 tgcccaggga gaatatcgga gaagtggtaa accgcggatt tgattttcag gtcacctaca
     5821 gggatgtgat aggacaactg aattatacac tttcccttaa cagcggttac cagaaaaaca
     5881 agatcagatt ctgggatgaa acacccggta ttcctgagta ccagaaatcc accggaaggc
     5941 caatgaatgc cggattgtac tacaaggcaa tcggcatatt caaggacgat gaagctctga
     6001 acggctatcc gcactggcag ggagcacgtc cgggagacat tatttttgag gacatcaaca
     6061 aggatggcaa gattgacggt cttgaccgtt acagggaacc aaaaaccgat cttcccgtgt
     6121 tttccggagg attcaacttt gacctcacat ggaagaatct ttactcttcc gtattcctgc
     6181 aatgggcaac cggtgctgtc aggaataact actatgagat gcagggagag gcaggaaatt
     6241 tcctggctga agacgccgca ggccgctgga ccgaacagaa tcccaatgca tcaaaaccca
     6301 ggacatggaa ccgttattac gggtactgga gggagcagtc aaacacatac tggctaagat
     6361 caactgatta catgaggctt aaaaatattg aagtcggtta caacattgcc tccctgccat
     6421 cagtaaaaaa ccttggaatt gaaagtctga gggtattctt taccggattg aatcttctaa
     6481 ccttagacaa gcttaaggat ttcgatcccg agtccacctc ttccacttcc tatccgctca
     6541 acaaggtgta taacataggt gtcagtttaa ccttttaata atcagaaaat gaaaagtttc
     6601 aagtttatat atttactgtt gccttttctc tttgttttag gcaattcctg ttctgaagat
     6661 tttctggaaa caaaaccact tactgaaata tcggaagttg acgtatgggg cgacccggca
     6721 cttgccaggc tttttgtcaa tcacatatat ttcaataccc cggaaccctt cagacggggc
     6781 aggctttcat cgaatctggt tgacgaagct gattacaggg gaaatacggc ttccctgaac
     6841 tttaacaacg gaatcatcac aatggatgca actcctacct ggaacaacat ttattacagc
     6901 aggacctggg cagatctgta ttcaaacatc aggtactgca attcattcct ggagaatgca
     6961 gacaagattc ccttcgatga taataccgag aaaaacaaaa tggttggcga agttcatttt
     7021 ctaagggcca acctgtatca tgaactggtt gccatgtacg gcggtgttcc gctggtgaag
     7081 agtacatacc gtctacagga tgacttcgag ataccgcgtg actcctatga ggattgtata
     7141 aaattcattg tggatgaatg caatatcgct gccggaatgc ttccggaatc ccattccggg
     7201 gcagatgtgg gccgggccac aaaaggagct gcaatggctc tcaaatcacg tgtcctttta
     7261 tatgccgcaa gtgacctgca caataccaat atgttttcat ctttctccga accaaaactt
     7321 ctgcaatata ccggaggaga ccggaaggcg agatggcagg cagcaaaaaa tgcagccaga
     7381 gatattatag atctcaacct gtacaggctc aacaatgcca atccctctcc gtccgattca
     7441 atagcaaaag actatgcgga ttattttata accctttcct ataccccgga agatatctgg
     7501 gcaaaattcg tggtaacgga atcaggtcag atgatcggcc tgtacagcct tatgaacggc
     7561 taccatggct ggggaaccaa ttgtccggta ggtgatatgg ttgatgccta tgaaatgagg
     7621 gatggaacgc gtttcgactg gaataatccg gctcacgcgg catcacccta caagaaccgg
     7681 gatcagcgtt tctatgcaac agttttctat gagggtgcaa aatggagagc acggcccgga
     7741 gatgtggcag agaaggatcc tctcaatgcc gttcaggtgg gaacatggga aaaatgggat
     7801 gaagccaccc agtctgtaaa ctatgttttc ggcctggata ccagaaaggg cgggatcgag
     7861 gaatggaacg gatcctatac cggatattac atgaggaaat tcattgaccc ggcctgggat
     7921 gtatcgttta ttgaatcggg gggtggcggc tatcagacag ttccctaccg ctttttcagg
     7981 tatgccgaga tactccttaa ttacgcggag gcatgtatcg aacttgaaga atatgacgag
     8041 gcccgcacct atatcaatat ggtccgcagg agggcgggac agcctgatct gacagaatcg
     8101 ggatctgccc tgcgtgaacg ttacaggaat gagagacgga tcgagcttgc ttatgaggaa
     8161 caccgtattc atgacgtccg ccgctgggcc ataggaccgc aggcatacaa gcccgtgacg
     8221 gcagcaaagg tagtgtataa actcctcccc gataaaacaa ccgcaacggt accaaccatc
     8281 acacatgaag tgttccagac caggaagtgg cttgacaagg catatttcat gcccatcatg
     8341 agggatgaat taaacaagaa cagtgctctt gtacaaaatc ccggatatta acaaaaccaa
     8401 agttgccctt ttaaagggca actttttttt aaatccatac cactcactgt taaataccct
     8461 ggaatcagaa attgaaaggg ttttaatgaa aattatgaaa accaaatctt aaaccggaac
     8521 aaatgaaaaa actgctgatt attttactcc tgctgccggc atactgccag acactgccgg
     8581 cacaggaaca agcctttaaa ataacacacg gtccctatct tacagacatg agtgaaaccg
     8641 cagtcacaat tgtctggatt acaaataaaa atgctcttgc ctgggttgaa gttgctccgg
     8701 atgacggaag ccatttctat ggtaaggaac gcccgagata ctatgatacc tatcttggcc
     8761 gcaggcaggc tgcaaccaca ttgcacaggg taagaataga aaaccttact ccgggaacga
     8821 gataccgcta tgggattttc tcaaaggaag tccttcactg ggaaaatgat gcgcggattg
     8881 tttacggcac aaccatagcc aatcctgcct acagcagaaa ttcactctca ttcaaaacat
     8941 tcagcagcag cgatgataca gtttccttta taatgctgaa cgacatccac ggcagggcag
     9001 acttcatgaa agaactctgc agggatatag atttcaacac catcgacctg gttgttttca
     9061 acggcgacat gtcaagtgcc attcatagcg aagaacagat attcagtgat ttcatggatg
     9121 ctgcggttga cctgtttgca aaaagggtac ccatagcctt tacgagaggg aatcatgaaa
     9181 cacgcggtcc ttatgccgac tatctcatgt actatttccc gcttaaaggt gaaaagattt
     9241 acaggacctt caatgttggg aatgttgcct tcataatgct tgactgtggt gaggacaagc
     9301 ctgattccga tatcgaatac agtggccttg ccgatttcga tgcctacagg aatgaacagg
     9361 ctgcatggct cagacaggct gtactggaaa aatcattcag ggatacccgt aacaaaatcg
     9421 taattcttca tatgccgccc ggagtcagcg actggcacgg cacccttcat attgacgagg
     9481 ttctggtccc gctgctcaat gaagccggaa cagatgcggt actcagcggc catacacaca
     9541 gatattcctt tcacccgccc gtgcagggca aaacagcatt tcccgtggtg gtaaacagca
     9601 acaacacata cctccgggga gatgttgtca acggtaagat aaggatcaga atgaccggga
     9661 cggcaggtac aaaaccgata gaacatattc tggaataaag gtttgtgaaa tgggaattcc
     9721 ttaaataccc atttgattac gggaaatttt taaaataatt tgcattatat caatttccaa
     9781 aactatgatg ataaaatcat tgagcaggac aagaatattt ttcttatcat ttgttcttct
     9841 tacatgccct ctataccttc tggatgcaca aaaggccggg gattccggac agccaggctc
     9901 tttctccttt gtttatgtaa ctgacatcca tcttgattac aatactacaa ccctgcagta
     9961 ttttgaccag gctgtaaaca ctataaatga actggatcct gattttatta ttactggagg
    10021 tgacaatata aaagatgcaa cacaaccacg cagatcctat gcagattcac tctttaatct
    10081 gtacctgtcg cagataaaaa gattcaggat gccggtctac accggtatcg gtaatcatga
    10141 atgttttgga ataaacaatc cggatatttc agatgaagat cctttattcg ggaagaagat
    10201 gtatgaatcc aggatcggga aaagatatta tacatttacc cataaaggct ggaaattttt
    10261 catgatcgat gacatcagga tcactgatac cgggcataag tatatcggtc atattgatga
    10321 tgaacaaatg gaatggctga agaatgaact gtctgcaact gatttcctta caccggttgt
    10381 ggtatgcagc catatcccct ttatctcttc aatgaaaaag tttgaattcg gttctttagc
    10441 cggcaatcct gataatgacg gagtgtccaa ttcaattgaa ttcttcaggc tttttgaaca
    10501 tcacaatctg aaattgctcc ttcagggaca tttccatttc tttgaagttc tttataccaa
    10561 taacatatat tatgtcacag gaccttccct gaatggaaga tacggaagca tgctgacaaa
    10621 aacagcagga tacattcttt ttaccacaga cggggatcgt ctgtcatggc aactgataaa
    10681 gaatgaacta taaaaaagat tgaactataa caataaacgg ggacatccac cggaatgtcc
    10741 ccgtttatga atccatatat cttaatctac tcctaacgaa ccattttcgc tgcagcgtga
    10801 gggaaaggag agtgaactga ttatatttca aaattatcac attcccggta tgcctggatt
    10861 accctggctt caccctcttt tgtaccccgc tggcttatac catgttccat tcccagtaca
    10921 ccgtcaaatt ttcgttcgta gatatatttg aacacattcc tgtaattaat ctcacccgta
    10981 gtaggctcat tgcgtccggg attatcaccg atatggaatg cagctatttc actccatgcc
    11041 gcccccatgt tcgggatcag gtttccttcg gtaatctgct gatggtacag atcattgact
    11101 atcttgcacg attttctgtt aacagcacgg cagattgaat aagcctgggg gattccggtc
    11161 aggaacaacc cgggatggtt aatcagtgtg ttgagtggtt ccagaaccag ggtaagtccg
    11221 gcaggttcaa ctatatcaca gcacattctg agattgtcga tcacgtttgc tgtctgataa
    11281 tcccggtgaa gcctctcatc atatctcccg ggaacaacaa gtgcccattt cacaccggtt
    11341 cttttgaagc actcaactcc ttcctgcatt tttttcgaaa gcatttccct tacttcctct
    11401 ttgttcagga caaaggaagt aactgaaaaa tcagcataca ggacaaaggg ccccatttcc
    11461 attcccagcc tgttcatttc acccgcgatt ctgagttgct cctcaacggg gcggttcatc
    11521 aggccgttgt caaacatagc cctgaatccc tgatcgtaac agaatttaat attatctacg
    11581 ggatcggtgc ccgcatgatg acggaacatt ccgatactgg gtccgtattt cagcttgaaa
    11641 ggggctgtgg catcaggaag tctttgcttt cccagaccga aggctgaagg agctgccaaa
    11701 acggcagctc ctgacacagc ggcttttttt atgaaattcc ttctggagtt gatcataatc
    11761 ctaagtcatt atgtgtaatt tattttctgc tgcaggagac tagctgctcc tggcggccct
    11821 ggctgcagct ctttttgctt cagcaccggc atcatatgcc tcaccggcaa tgggaacttc
    11881 ctttggaata tcaaccttgc cgaatgcaaa taccttggga ccgagtttca ggtcggagtt
    11941 catcatttcc tcccaggttg tctccctgcc cgtataagcg gaaatacgtc ccataatccc
    12001 gaccattgtg gatatggcag tattttcaag ctcattgaaa ggctggttgt tgcggatggc
    12061 tgtaaccatg tcgatatgtt cctgcacata gggatccatc atgcttctgc ttgaaggatt
    12121 gccttcactg tcaagaggat attgatattt ccatatctcg ttgccggcaa ggtcatgaat
    12181 ggtatccgca cagtttgtcg agccctttgt tccctgcagt ctttcagcaa cgctgttcca
    12241 gcatccgttt atctgacggc actgactgtg gaaacgccgc ccgtcttcaa aaataaattc
    12301 aatggagaaa ttgtcatact ggtcacccgt gacccgcctc aggcgagaac ccatgccatg
    12361 ggccttaacc ggatgcgaac cggtgaacca gttcatgacg tctatattat gaacgtgctg
    12421 ttcaactatg tgatccccgg agagccatgt ccagttaacc cagtcacgta tcatccattc
    12481 catttccgac catgcgggat tactgtcacg gtgccacagc tttccgccgt tccaccagat
    12541 gtttccgcct actatgtctc cgattgcccc ggctttcacc tggttgtagt tggccacata
    12601 gttacgctgg tgccggcgct gtgttcccgt aacaatggtc agtcccagtt cctgggcctt
    12661 aagggcggta gccatcactg acctggcacc tgtgggatcg acgcataccg gtttttcggc
    12721 aaaaatatgt ttccgggcag ccactgcggc tgccaggtgt gccggacgga aatatggcgg
    12781 ttcagcaaga atcactatat caacatccga tgcaattact ttttcgaaag cgtcaaatcc
    12841 gacaaagcaa ttttcttcag gcacctcctg gcccttctgt ttgagaattg cactttttgc
    12901 ctgctcaaca cgatccccga aagtgtctcc aagtgctgtg acctgcagat ttggacccgc
    12961 attgaggaaa ttaatggctg ccccgcttcc gcggccgcca cagccgataa caccggcttt
    13021 cagtaccggt ccgtcgggtg ctgcatccag ccatgtgcca tcgggtaccg gtttaaaggg
    13081 tttgtccttg cctgagctgc agcctgtaat gataacaggt gccacaatac cggcagcccc
    13141 tgcaacagtc gtgcccagaa aaccacgtct tgagattcca gattttttca tatgattttg
    13201 tttttatttg aggttaaata tactaacaat caagccgggc agaagtaatc gaatttaaat
    13261 atatgaattt tataaacaaa ttttcaccgg ccgcttaact atttttaacg gggatatgcc
    13321 tgacaggcat aaataaaacc ttctgcggtt ttattccggc agccatcacc gcctacacca
    13381 cattcagctg aaggctttcc tgccggcgtc tcacttccag tacctgtgtt gttcctccgg
    13441 cctcaagatt aatgtcatga ggcttgttgg tcttccatgc cccgcaggta aaatccggaa
    13501 tatctatcgt ctgtgacctg ttggccacag accattcgct cagcggggcc accacgctcc
    13561 aggcagcacc gtcatatacg ttctggtcaa ggggaagtcc gttgcgcagg cagtcaatca
    13621 tcctccagtc catgataaag tccattcccc cgtgtccgcc aaccctcctt gccatctctc
    13681 cgactctctt tattatttca ggtgtgtact tatcccgcag ttctgccact tcctcgtcct
    13741 taacccagct ttccccgaaa gcaattctct cgacaggata tttcacggcc attcccgttg
    13801 ttcccttcac cagatgtatc ctggaatatg gctgttcaga agacacatca tgctgcacct
    13861 taatggtttt cccgagtgcg gtgcggataa gcgtggtatt gaaattcccc ctgaacctgg
    13921 aattcttcca tggttcataa aaggaatccc ctgccacgag ttcttcttcc ctctgatgca
    13981 tcgtgaaatc gtttgttgac atggaagtca ggtaatccat cctgtctccg cagttaatgt
    14041 tcagaacctg tgctattggc cccagaccat gggtgggata taatatgccg ttacggctgg
    14101 cattctcttt aagcctccac atcccggtat aggccccgtc tgactgacgg tcatctatgg
    14161 gcttcttgaa attcattccc agcaggttgt gtatatatgc gccttcccca tgtattatat
    14221 caccgaaaag tccgttgcgc gccatgttca gtgtcagcat ttcaaaaaag tcatagcagc
    14281 agttctcaag catcatacag tgtttccgcg ttttttcaga tgtttccacc agcatccatg
    14341 cttcatccac agtttttacc agaggcacct cacataccac atgtttgccg cattccatcg
    14401 catagtttgc cattgccgca tgcatgtccc atggtgtaca gatatatata agatccagac
    14461 cgggaagctc aataagcttt ttccatgaat cctcatcacc gaagaattcc tgagccgccg
    14521 gacggccgat tctttcaaga taacgctgtg accctttgac tgcagcctca cgaagatctc
    14581 cgaaagcagc tatttcaaca ccttcaatat ttgaaagcct ttgaaccgca ccactgcccc
    14641 ggtctcccaa tccgacaaaa cccgtacgga caaccggcag ggcaggcgct gcataacccg
    14701 acatgttaaa atgcatgctg tgtttctttc ttgctgcagc aatgatttcc ggaagatttg
    14761 actgggcttc acgcggtgca cagctctgaa tcatattccc cgctattgct ccggctccgg
    14821 caatagctgt tgatttcaaa aaatcgcgac gactggtttt catggtaatt cctcttttcc
    14881 tgttaactga tcaatttatt ttttaacaat tcaagtgtaa atgtacatga aaaatcagga
    14941 ttatgacagc agcaggcgaa aaatagacac ccggaaggaa gtggccgggg agcagcggct
    15001 gatccatccc cacccggagg gccgcaatag aacatgaata aaggctgctg ttccataaag
    15061 gattctgaaa attttttctg aaaaagtgtt gccgcatgtt tccaaaggaa taacggcatc
    15121 cttttgcaaa aagtttttaa atttgtagtg atacctgaac cctaatgccg gaatccctga
    15181 aaaatatcac atgccttttc ttctttgttg ctctgattcc atgcattccg ggatgcagga
    15241 ctgaatctgg aggcgacctg cctgaatctg acagattccc tcgtatcagt ccggattaca
    15301 cggatatttc aatcccgtat aacattgcgc cattgaattt cgtggtaaag gaaaagggtt
    15361 ctgcgtattt cgtcagaatc agttcagaaa atgataccgc catcagactg aaatcacgca
    15421 aggctgcaat ccggataccg cagggaaaat ggaagaagct tctgtctgca aacaggggaa
    15481 agcaattaaa catcgaaatt tatgcaaagc agaagaaata cggctggata aaatataaaa
    15541 cagtcaccaa tgtaatatcc tccgaggccg tcgatccctt tgtgacttac aggctcatcc
    15601 ttcccggata tgagagatgg agcgatattt caatccggca gcgcgatatc cggggtttca
    15661 gggaatggcc tctgataaaa aacagtgttg ccgaccggaa ctgtgtcaac tgccattcct
    15721 tcaacaacgg ccggacagat gatttccttt tccacatgag gggcagccgt gccggcactt
    15781 acttctactc agaaggaaaa cttataaaga tcaatcttaa aacacctgag atgaaaaacg
    15841 gcccggttta tcccaggtgg catccctccg gaagatttgt cgccttttct gcaaacaaca
    15901 ttgtacagca attccattct gccaacggaa acaaggttga ggtaagcgat cttgaatctt
    15961 ccctgcttct ttatgatacc ggcaaaaatg agatcatgcc ggtggatctt cctgaccgcg
    16021 ggaaatatat ggatacatat ccggaatggt ctcccgacgg caggatgatg tatttctgca
    16081 ggacctcccg tctggaagag aactatgatt accgtcatac ccgctatgat ctctacaggg
    16141 tttcctttga tccctcatca cgccgtttcg gagaacctga acttgtaatc aatgcgtcgg
    16201 agaaagctaa aagcatttca tttccgagaa tttcacccga tggcagattt ctcgccatga
    16261 cgtaccatga ctatggctgt tttccggtct ggcacaggga agctgacctt attctgctca
    16321 atctggaaac cctggagatc ataaatcctg aactgaacag tgatttccct gaaagctacc
    16381 attcctggtc atcaaacggc aggtggctcc tgtttgccag ccgccgtgac gacggcctcg
    16441 caaccagacc ctatctggcc tatgtcgggc aggacggtaa atcgggaaaa gcatttataa
    16501 tgccccggag aaatccggaa cattacaata cttttctaaa aagttataat ctgccggaat
    16561 tttcaaatct gaaaattaca atgaatccgg gcagaatacg aaatgtcgtc acctcaggag
    16621 ctcttcaggc tgaatgggga ggaaaatagc agaggatgaa aattaaaagc gcaaaattaa
    16681 ccgggttttt ctcagtcctg ccttccagat tgttcttcct gtttgccttt ctttactttt
    16741 actttttcgg aaatcatgtt ttgtttttcc aggaaaaaac cagtcttttt attttcagtt
    16801 tcgaattcct gaccgaaaac attaagcagc caggtggttt tcttgtttat atttccgcct
    16861 tccttgcaac attttcctgt tatccggcag ccggagccct gatatatgca acaatactat
    16921 ggatgattgt ggttttctcc cttgaaatca ccgttgccgt gagcggatca aaaagagttc
    16981 tgatacctta tctggccgga cttgctcttt tctatcttca gcttgactac aggtatttgt
    17041 tgttcaacaa tacgggaatt ctgctgcaga ttcttttttt ctggctgtct gtcaggtatc
    17101 tgaaaggatt tattcctgtc ctgctgcttc ccctctttta tttccttacg ggaggattca
    17161 cattgattta tctttttatg ttctctgcct ggctccttta cacaagaccg gaggcatgga
    17221 aactgaaaat gctctccttc tggctgatta tcttgttatt tttcgggtta tcgaaggaat
    17281 ttctgttttt tcagtctgca gatacattgc ttaaataccc tttcacggaa gcggatacag
    17341 gttcacagat atatattttt tctgcggcag cgttcatgat cgccttcctg ccattattgc
    17401 cggctttcag gaaaaagaag ccggtcagga ccgaaatatt caggcttcct gcgtcactta
    17461 ccggatctgc catcctgttt atccttctgt catccattgc cgcactccgg tatgacaaaa
    17521 agaccagtca gtatttccgg gtggaaagcc ttttttacga aggcaggtac caggatgtta
    17581 ttgattacaa tatcagacat ccctccggca acattcttac actcttcctg aacaatattg
    17641 cattgtgtga aacaggccgg cttaatgaca tgctctttca cttccggcag agccctgatg
    17701 gcggcaccct gttccagaaa tgggatatca gcagtgaaat atccagaaga ggaggttatt
    17761 tctactatac catcggcatg atgaacgaag cacgccgctg ggcatttgaa aacatggtta
    17821 ccagggggtt tacgccggaa gggctcaggc tgctgatcaa agcagacatc ataaacggtg
    17881 attatcatac tgcagcgaaa tatataagaa ttctgaagaa aaccctcttc tacagggatg
    17941 aggcaaggca atttgaaaaa ctccttttcg atgacaaagc ggtggatatg catccggagc
    18001 tgggcgaaag gcgcagaagc aaagcccgga cagactttat tacaatttcc ggaatccccc
    18061 tgataaatat tgaaagaatg ctggcaaccg gttcggtgaa ccggaaagct ttcgaataca
    18121 agctggcctg gctgctcctg acaaaagatc accagggcat tttacaggag tgggacaaac
    18181 taggtgaata tggctttgaa aaagtgcctg tgcatatgga tgaagctgca gcagcggtaa
    18241 aagtcctttc tgccggacaa tttattccgc agggagattt cacggtcagt ccggatactg
    18301 aactccgttt tgaaaggttc ctgcaaacct tccgggctta cggcaataat ccgaaagctg
    18361 ccgaacctgc tctcagaaaa cagtttggaa acacattctg gtactggaat ttttatcact
    18421 gaccgctgtt catgaatttc tctctgcctt ctcttcagcc ggaatagccg ggatgattcc
    18481 cttccggatg taatctgcag atgatgaaaa aagccttccg tttccggaag gccgtttcct
    18541 tttaagtgat ttcggataat tccctgaaaa actaatatcc cggagcctgt tccagctgtg
    18601 gtgccttatt gatttcagaa cgggccagcg gtatccagta aaatttgtta tttgcccagg
    18661 atctgtcatc tggcttggca gacaaatcat attcccactc ccagtctccg ttcacgaatt
    18721 ccctcacctt catctcataa acattgccga tcacatcggg agcagtcatc cagcggcgca
    18781 tatcatacca ctggtgtcct tcagcaaaat gctccagata tctttcatgg cgaacccatt
    18841 tccttgcttc agcctggtct gttgtcaccc ggtcaggcag ccctgcacgg ttgcgtacca
    18901 tgttcagcgc atcgagtccg ttctgcagat cgccgcctcc tatttcgatg caggcctctg
    18961 cgtaattaag aagtatttct gaataacgga attcaaccca tgtatttgta ttgtaataat
    19021 actgacctac agtggctgga tccataaact ttttcagata gtatccgttt ttggttccgt
    19081 tccaggcttc aatctgaccc tggcgggtat ccacgccata ggatttcaca gtgccatcca
    19141 tgttgtacaa gtgcccggtc tgtaccctgt ctgtggggtc aaatgccgcc ctgtctgcag
    19201 gacttgcctg ccatggtgcc ccgtgataca gtaccgttcc gtagaagcgt gcctcgcgtc
    19261 ctgcataggg atttctctgt ggttctgcgg caagctgggc agcagtagcc ttccggagca
    19321 cctggtttcc gggatcatac ctgtcccatt caaagggagt accgtcagcc atttcatact
    19381 gccgtaccgc cggttccgta ggattattgt ttccccagtt atgatatccg cagggaccga
    19441 accagatatt ggccctgttt atatttccgc cggtcagggg gtattgaatt ccccagatgg
    19501 tttcactgtt ccatgcaccc ttctgattga atatcccgaa atatgtttcg gcaatagcct
    19561 gaacctcggc ttcggaatat cctcccggtg gcgggttact gatatttccc acgagagaga
    19621 atgttccgta gtttccgtcc atcacggcct tggccgcatc tcttgctgct atccagcggg
    19681 cagcctgacc gcccgtcccg aaggaaacaa gggttttccc cgcttcgtat cccccgttaa
    19741 ccaggtcgct tgcacagaac aacaatactc ttgatttgag tgcggctgca gcagcacgtg
    19801 tggcacgtcc ctgctgcatg gtttcgggaa gattggcaat ggctttgtca atatccgaaa
    19861 gcacctggtc aagagtctcc tgcagggaag atcttttttt cgaaaggaaa tcacctccca
    19921 gctgaaaggg ttcggtagca atgacagccc cgccgtaatt catcagaacc agcgcatact
    19981 caaacgctct gatgaaatat gcttctccct tgatctggat tttaagtgct tcctccgttg
    20041 atgtgggagc gggtacattg tcgatatttg ccagaatggt attgacgttc tgaatattct
    20101 gataaataaa aggccatctg aggaagccgg cataacctgt attcgcaaaa tatcccagct
    20161 gatcgggact catcgtacct ttcaggttga catagttggc cggacggtga atacataagg
    20221 tctgatcggt tgccgaagac aagagatctt cacgcatgcc aaggataccg ttatcagtat
    20281 tgcctcccat tctgtcataa catttgccaa gataagcctt ggttacattg acatctgcaa
    20341 aaactacctg ttcgttaaag gaactcacag ggtcctggtc cagaacatcc gtgcatgatg
    20401 caagcagaaa cagcaggact gatattgcgc ttatataact taataacttt ttcatactgt
    20461 tatatttttt atttctcagt taattaaaag gaaatatttg caccgaacgc aaatgttttc
    20521 attatcggat acactcctgc gccgttggct tccggatcaa atttcctggt tgcggtccag
    20581 agcagtgcaa ggttgttccc tgagaaataa acgtttgcgt tagagatacc aatacccctg
    20641 aatatctgac gtggtatatt ataggtgatt accaggtttt tgagacggca gtatgcagta
    20701 ttgtcaagcc agtaggtact catgttcacg gcatgggccc agtagaaatc gttacggtta
    20761 aaggccctgc cgatatttgt gtttctgttg tcaggtgtcc agcggttgtc ataggtccat
    20821 ttgaaataat tgcctgcctc accgcggcgc tcgtcatagt gattgaattt gtagaatttc
    20881 ccctggccct ggaacatcag ggacaaggaa aggcctttcc agctggcgtc aatattaaat
    20941 ccgtaatagg ttttcggcac atcaacctca tcaatcagta tcctgtcatt gccgtcaatc
    21001 ttgccatccc cggaaacatc ctcaaatata agatcacccg gctttgcacc ggtccaggca
    21061 ggatagttct tcaccgccgc atcatcagca aaaacaccta tgcacttata catgagccag
    21121 gcattgtagg gatgacctgt ttctctctgc catggaaccg ctctttcagg ttcatccttg
    21181 aaaaccacct tgttgtggtt atagctgaga ttgcctgata catccagccg gaggtcggct
    21241 gtaagatttt tatgtactcc tgcttccatt tcaaaaccgg aattgtcaac acgggcaatg
    21301 ttttcattgg gaagcgacag acccgtaaaa tccggtacag atgcatccct cggggcaaga
    21361 atatcttctc ttttattctt gaaaaattca aaattccagt gaataaggtc attcagccat
    21421 tttgactcaa atccgacatt cctggttgtc tgtgtttccc atgtaatatt cgggtttgct
    21481 attgtcgggg ggccgacaac cgactctatt gcagcactcg ttccgaacga cattccgccg
    21541 cccagtccga aactgttcat gtactggaag gctgttccgg ggtccatacc catcttgccc
    21601 catgaagccc tcagtttaaa atagtttatg aacggaatat tgtctctcca gaatccttct
    21661 tcggaagcac gccatcctgc catgaatccg gggaaataac cccagcggct gctgggcggg
    21721 aatttaaggg atccgtctgc ccggaacagg gcctcggcaa gatatttccc cctgaaatcg
    21781 taggtcacgc ggccgatata tgatttgcgg gcatatatgg atgcctcccc gtcagtattc
    21841 ttgttaaggt ctgcccccgc attcatggtc tgtatcaggg atgagatgta aagttcgcgg
    21901 aatcccctga accagttgta atcattggtg aactgctcaa agccgccata gacgaagatg
    21961 ttgtgatcat aaaatttcct ggtgtatgtt acgccaaggt ttatggtctt gttgatggtt
    22021 ctgtcaaacc tttcagtatt ctgcggtgaa gaaagccccc tcaatgtcgg tttcatgggc
    22081 atatcggtca cgaatcccgt atgcgggtca ataacagcct cgtcccagtt cgggtaataa
    22141 agaacccagg gcttgtaaaa ggctttctgg taataatttg tcaggtcata gctgaatatt
    22201 ccgttcagtg acaaaccttc cacaaaggga attgtcaaag tggtattgaa tgaactcagc
    22261 agcctgtagg ttttctgatc attctttccg ccttgaaaag tagaggttac aacggggtta
    22321 tccccgtact caatatcagg tccgggaagg ccgttgggcc agaatgacca gttggtcggc
    22381 acaagccgtg ttgactggcc tacaatggca tcggcactct tgtagggata aaccctgttt
    22441 atctggaacc cggccagttc aactcctgtt ttgaaccagt cggttatggg catgtcgact
    22501 ttggcacgga cattatactg actgtatttc gtggaggact gcttatagaa tgattcatcc
    22561 cccttaaggc cgacggaaat ataatacagc ataccttttg tgcctccgtc cattgtcagg
    22621 ttatggcggt aggtcgtggt ccatttttta atcagatcgg cataccagtc tgtatccgga
    22681 tgcctccacg gatcggctcc tgatctgaac agctcaatat ccttatctgt ataggtcctg
    22741 gatttaccgg ttgccacctg gtattcggaa agcattgtgg catattcggc agaattgcaa
    22801 acctgcggga taaccgaggg agtctggaag ccctggtaga actggtaatt aagcctggtt
    22861 ttcccctcag tccccttttt cgttgtaata agtatcacac cgtttgcagc ctgtgcaccg
    22921 tatattgctg cggatgcatc cttaaggact gaaaggcttt caatgtcatt cggatccact
    22981 tcatccatat tacgtccggg aacaccgtca atgattacca gaggtgcagt ttccgatgcg
    23041 gccgagctcg gatttctgcc accgccaaga gtgctgcggc cgcggacaag gatccttgtc
    23101 ccgtactgac cgggttctcc gttgttctga ataaccactg aaccgggcag acggcctccg
    23161 agtgaagttg atacggtagg agccggcact gcctgaatac tctgaccgga tattgcagtt
    23221 acggagccga taacgtttgc ccgtttctgg gtggaatatc ctaccacaac aacctcatca
    23281 aggtcggtga tttcagctgt cagctcaaca ttgacgacag atctgccgga caccggaacc
    23341 tcctggctga taaaaccaat aaaggaaaag accagtgttg catttgcatt aacaccagca
    23401 atctcatatc ttccgtcaga atcggagatt gtgccaagag tggtacccct gacaacaata
    23461 ttcacaccgg gcatggcctg tccggtggca gcatccgtta ctgttccgct gactcttacc
    23521 tgctgttgca tgtcagcagg aatatgatca ggggaaaggg accaggccgg catcaccatt
    23581 cccatgacca gcaggaaagt cagaattttc aatgtcagcg acaagctgtg aaaattcagt
    23641 tcaggcccgt cgggccagaa actactgata gtttttttca taccactacg ctttaggtta
    23701 ataaaactaa attgcaaaat cgggaaatct cactcccagg tttataagtc tatggagctg
    23761 gtaaattaag attttttatg agaataaaaa aattttatac ctgatttcag agacctcaca
    23821 gggaaaaaac cggacaggca gaaaacttcc gggagccgaa ggagcttctg catgaacggc
    23881 agaagcctta agatatatgt attacatttt cagttaccgg gaagctatgt ttccggtgtt
    23941 ttcatcgaat tcacaaacca ccctgaagcc cacatggtag cagtctgaat accaccatat
    24001 actcttcggg atctgggggt cggtcctcag ccattcatca gtctgcgtat gcccgcgggc
    24061 ggcacttctt acttttccgg ccatatcctg gaaagatcct ccccgtatta catgttcttc
    24121 gcctgattcg ggtccctggg gatccagaat aatttcatca gccggatatt cggaatatgc
    24181 atcttcctgg taccagtcgg aacagaattc cgcaacgttg cccagcatgt tcttcaggcc
    24241 aaacggattg ggtttaacca tatcgggagt ctgggtcttc gacgggctgt tgccctgaaa
    24301 aataacatag gaatttatga cagcggtatc attctttgaa aattttgccc taaatcctgt
    24361 tttttcaaaa ttatcaggat cgcccggaaa gaaatatggt gtttcagttc cggccctgca
    24421 ggcatattcc cattcagcct cggtaggcag gcggtaagtt tttcccgtca cctgtgaaag
    24481 ccatctgcag tatgtctcgg cagcatggtg ggtgaatgag attgcaggcc tctgccccat
    24541 tccccatccc tggtcaggct gaccatatgg cggggtggct cctgatattg catctgtttc
    24601 ttccgcctgt cccgatctta ttccttccgt atcagtggtt ctcccttccg cagatgtctg
    24661 tgcataaaaa gcaaggtatt catcccaggt aacttccact tcagccatga agaagggact
    24721 cagttcaact tccctcaccg ggccttcatt tgatcttctg aaaggttcgc cggcagggct
    24781 tcccattctg aatctgcctc cgggaatggc tatcatattg aatgacacgg aagaattggg
    24841 aatctgttcc gtgaatgatt cgaaggcact cacctttgcc gctgtggtaa acaattcctt
    24901 tggagctgat gataccgcac cgaatgttac attgctcccg gtggtatagc cctctatatg
    24961 gtgcccggaa ttcagatgac agttgataca gtgcaggtcc ggtgtctttt ttgtctgcct
    25021 gtagtaaaga tgagcatctt ctccttcctt cgtcagctcg ggcgggaaaa gattctcatg
    25081 gcattttatg cagctgcttt cataggtatg aagccttgcg tgttccagcc ttccctttgc
    25141 atcccagtca aaggatgctg agtcctttgt ccagtaggac cagaggtcgc gcaggccggt
    25201 agttgccttt gcccacagat atccctctcc cttgggggga agatgacatt ccgtgcagcc
    25261 gacacggtag ccggattttg tcacgtaatg tacggatttt ttccagctgt tatcagcccc
    25321 ggggtgaata tggcaggaca tgcagtattc atttgtggaa gttttctcga tcattaatcc
    25381 gcttccgagc acaagcagga tccccagaac cagtccggga gtgaaccaaa taaggaactt
    25441 cttcatcagc atagtaaatt agcaggtgaa gataaaaaat atttctttta ataatacgca
    25501 tcccagactc tgtatccggt cctgaattct gtttcttatt tttaaatttc tatttttacc
    25561 gggtcaatac atctgtaggt atagtatgga aagaatctta tcaatccttc tcgcattgtt
    25621 gattactgcc gttgtggatt gccacccctg gaagccaaat cattatgtaa taatcgatac
    25681 tgatggcggc atagacgaca tcagggccat aacaatgttt cttgcatccc ctgatgtaag
    25741 gattctggga ataactacct ccggcggggc tctttctccc ggaaattcct acataaaagt
    25801 gaaaagcctt ttaaattcaa tgttccatgc aggcattcct gtcggaagcg ataccggagg
    25861 cagctacggc atgaaggaaa tgccctttgc cctgcaggcc cgctggggca gtgaagacgg
    25921 aatctcgcca aaggaagccc cggataatct tgatattatc tcttccctgc ttaaagccga
    25981 aaaaacaaag atctcctttg tatgcctcgg cggcatgcag accgccctta aagccttcag
    26041 gaacattcct gagttcagga cacaggtaaa ggatattgtg tggagcgtca atgaatcggg
    26101 atacaggaac ggattcaatt acaggatcga cagggaggcg gctgatgcaa tgctgaaaca
    26161 ggagataccc gtgaggatgg tcaggaacat gccatccggt cagaatgatt tctggaatga
    26221 cggactgata agggatctgg ccggaataaa aaatccctac gcggcaaagc tctctacatt
    26281 tttcgccaat gaagacttca gatcccacag gttctcattt tccggaagcg atgagatggc
    26341 agccgttttc atacattatc cctcactttt tgtgaacaag atcacaggaa gcatttcaga
    26401 aagcacacca gcggatattg aaggaatcag ggaaggcaca ctgaaaataa tcagtatgaa
    26461 aaccattgag gaaaaccagg tcattaagga acttcccctt gatccggctt tctattttga
    26521 cgatctcagt cctgtggtgg aagagattat tgagaagcac ggtgttgagg aatggaagtc
    26581 aggtatcttt gccagtgaga tgcaccgcca tctgggaata tttgaaataa taggcgtaaa
    26641 aatgggtata cgcgcaagag aatatttcaa taccggtgtt gatgaattca gggctgtgtc
    26701 ctatgccggt tcagtacaac ccatgagctg catgaatgac gggtttctcg taagtaccgg
    26761 ttccactccc ggacatggcc tcctgactgt gagaaatgat acggccctca tcccgatggt
    26821 tgatttcata tatctgggaa ggaaataccg gataaaactg aaatcagaga ttacaacaaa
    26881 aataagttcg gagcttaagg aaataaactt catttacggt ctcgacagca atatctactg
    26941 ggagcttgtc cgcaagaata ccattaaata ctggagggat atggacagac atgatatatt
    27001 tgagatcggg gaactgaact gaacaataat gaactgaagc agaatcttct ttcatcatcc
    27061 gttcctgccc cggtatccga ctaaaagtcc cgataagtcc cgccggcaga taatcccgct
    27121 cccttccggc cagaattaaa tatcacttat tgtattcatc aaaaaaatca ttccccttgt
    27181 catccgtaat gatgaatgca gccatattct caaccctgat ctttctgact gcttccatgc
    27241 ccagttcctc aaaatccaca acttccaccg atttaatatt ttcggcagcc agtactgctg
    27301 ccggtcctcc caccgatccc agaaaaaacc cgccatgctt cctgcatgat tcaatcacgg
    27361 cgcggctgcg gtttcccttg gccagcatga caagcgatcc gccgaggctc tgaaacagat
    27421 caacgtaggc atccatacgg ccggctgttg tcgggccgaa acttcccgat accatacccg
    27481 gtggtgtttt tgccggaccg gcataataca caggatgatt tttaagatac tcaggcatag
    27541 gttttccttc atccagcatc tgtttaatcc tggcatgcgc aagatccctt gcaacaatca
    27601 gggttcctgt cagactcaaa cgtgttttta ccggatgcct tgaaagctgt gcaagaattt
    27661 ccttcatagg cctgtcaaga tctatttcca cgggttcctg cagtccggga gccttgtcag
    27721 gcataaagcg ggcagggttc ttttccagtt cctcgagaaa aataccctct tccgttatcc
    27781 tggctttgag attacggtct gcgctgcagc tgacaccaag tcctgccgga caggaagccg
    27841 catgcctcgg taaacggata accctgacgt catgaacaaa atatttgcct ccgaactgtg
    27901 ctccaacccc gtactcccgg catatcttct gaatcctttc ctcccatttg ggtaccctga
    27961 atgccctgcc gccttcactt cccgttaccg gaagatgatc aagccagccg gccgtagcca
    28021 gctttacggt tttgagtgtt tcctctgccg aagttccgcc tatcacgagg gcaagatgat
    28081 agggcggaca ggctgaagtg cccaggtcct gtattttttc tctgacaaac cttgtgaggg
    28141 actcatcatt aagcagtgat ttgctttcct ggtacagaaa ggttttattc gccgatccgc
    28201 cccccttggt gataaacaga aactcatact cattcccctc aacagaacag atatcgattt
    28261 gtgcaggcag gttggtacat gagttctttt cctcaaacat cgtgtacggc actatctgtg
    28321 aatatctcag gttcttttcc tgatatgcct taaaaatgcc tttcgacaga tgcatcgcat
    28381 catcggcggc tgtccatacc tgttcccctt tttttgcaat aacaatggca gtgccggtat
    28441 cctgacacca gggcagttcc tcctttgctg aaataactga gttcctgagc atggtataag
    28501 ccacaaagcg gtcattgtcg ctcgtttccg gatcatcaat tatgtcccgg agtttccgtg
    28561 tgtgggatgt tctgaggaag aacgaaagat cggatattgc cacggcggca agaacttcca
    28621 ggcctcgagg atccagctgg agtatcttca ttcctccggc ttcggttgtt tttacataat
    28681 cctttgtcag aagccggtat tctgtttcat ccttttcaat ctgaaagggt ttttcataaa
    28741 taaattttgc catatctgta ttttttctga aaagtcttta ggggcagcag ttcaattata
    28801 tatccgaaag cttaaacgta ttttttgccc ttatccccct ggaagtcata tgaagatcac
    28861 agcattcaac atgccggtcg tgctgaatca ccaggatata atcgttttcc agtgcttctt
    28921 ccaggatctt tttcttctca ctgatggttt taagtgattc tatatcatag ctcatattcc
    28981 atatcagggg aagatgtgcg gcagtcggaa tcaggtcgcc tgcatagaca agtgtttttc
    29041 ccctgtaatt cacaagggct atcatcagcc cgggggtatg gccgtagcat atcctcactg
    29101 aaaaatcctt gaacagctct ccttcttcat caatcaggtt aaggcggccg ctgtcatata
    29161 ccggaatata attctcttcc aggaaggcat cctcctccct gggattgttt ctcagggccc
    29221 actcccactg tattctgctg acatggtaaa ctgcattggg aaaaacaagc tcgtagccag
    29281 tactcttagc gttttttctg acacagccgc cgcagtgatc agcatgaaga tgggtcagga
    29341 atacatcagt gacatccccg ggactgtaac catgcttcag cagtccttcg gtcagtccgt
    29401 cgccgttgtg aaggtgtaca tgcctgaaga atttctcatc ctgcttgtcg ccccagccgg
    29461 tatcgacgag tatcacacgg tcacccgtgc tgacaataag cgacctcaga gaaagagtga
    29521 taaggttatt ctcatcggat tcatagaccc ttgaccacag aacttttggc accacgccga
    29581 acatggcacc gccgtcaacc ttaaaatttg caatattaaa aagatgtact ttcattgttt
    29641 cagcagttat tctgtcagcg gggccgggtc tgactgtttc cctgtacctt gtcaactatc
    29701 accttgtaat atcactggcg gtatatgata tccgcggttt actctttttt gtcgtcatct
    29761 gatatgttat cctcataagc agtctgagaa cctggtagga aagccagccg gcaaactgcg
    29821 aataccatct gcgcctccgg ctgtactctt caagggtaat ctccctgcag tcattcctta
    29881 ttatttctgt cagctccttc tcgaaatttt ttgtgaattt atcatcgagt attcccacat
    29941 tgatctcaat actggcataa tcactcaggt ggttcatgtt atagctgccc acggtgcacc
    30001 acatcccgtc aaccgttgcc actttggcat gaagatttga cgggaggtat tcataaatcc
    30061 tgatcctgct cttcagaatg aaatcataca ggaattcggt tgccttttta aacacgggtg
    30121 catccgattc ggcggaaaat acaacggaaa tatccacacc tctcaggccg gcatttctca
    30181 ttaactttct ctcatttccc gcaggcagga aataagagtt aaaaattatt attctggaac
    30241 cggccagacg gaatatctca cggtaacttt tcgatatctc catcttgttg cggtaccagt
    30301 tgttctgaag taccttcagc ctgatatctt ccctgtagaa taaatgtgta tcaacagttt
    30361 cctgggatct ttcctctttc gatataaagg ttttattcca gagccttttc aggatcgaaa
    30421 gagtatgaac acattgtggt cccctgaaca gtactgcaaa gtcaagccat tcctttttgc
    30481 ggggggaacc atgatagcgg ttggcaaagt tcatgccccc tatgatggcc gcttcaccgt
    30541 ctgccagtac cacttttgaa tgaagccgga gattgagctg aaagcccttt ccggtaaaag
    30601 taggggaaaa gaaacggaaa agaattccgg aattctcaat gctgtcaata aaatcttttg
    30661 aaaggtattt tgttccgtat gcatcaagga gcagataggt acggacacct ctttccgcag
    30721 ccctgatcag tgcctttacc attctggttc ctgtttcatc ctcgtccaga atatatgtct
    30781 ggaaatgaat gaaatgcctt gcttcatcaa tgattttttc aacagccgcg aaaaaaggct
    30841 caccaccccg gagcagttcg acataatgtc cgcccttgta gccggaaaga cctgaaggtt
    30901 ttttcataaa tgtgctgctg agttaacctg atacaaatat aagtctttaa agtaactgta
    30961 atgaagtctg actttgagaa acaacagaca acaggtttta atgccctcac ttctcttcac
    31021 tgtttcagcc gtcaactcaa aagctcacgg tacaggacaa ttcagaaggg ctgttttcgt
    31081 ttatgtaatg acatatacgg atgcggacaa tcagctgaca aaattgccgg agaatataac
    31141 ggcattgaat ttgcgggaca gcggcagact tacaaaaaaa attaaataat caatcatgaa
    31201 aaaaacattt ctactactgg ctttcacggc tcttatcggt ttacaggttc acggacagga
    31261 ttacaaaacg agtgcaggcc tccgggcagg ggttccgttt ggcctgactg tcaaacactt
    31321 tctgacaaaa accaatgctg tggaaggaat tctggccagt acaccctggg gaggttttgt
    31381 tattaccgga ctatatgaaa atgaacactg gacaggcaaa tatcccggtt taaactggtt
    31441 ctggggtgcg ggagcacacg tcggtttctg ggacagaggc aggaacaatc cctatttcag
    31501 cgactcctac aggggaaatg cagtgctggg ccttgatgca ataattggtc ttgaatatac
    31561 ctttgatgat attcccctga acctgtcact tgacctcttt cccaccttca atattttcgg
    31621 atataccggc tggggaggaa taaacggagg aatatcagta aggtatgttt tctgaatttt
    31681 tcagggcttc atgccatata tatccggctg aaaagcagac aggcagaaat aaaggatatg
    31741 tgaaaacagt acttatgccg gtgcagttca gtaaataatg aaataaatta tcctttttac
    31801 cagtaaatat tttgaattat tcaaaaaacg tgttttcttt gcatccgcgt tttttaatct
    31861 tttaatggtc cattcgtcta gtggttagga cgtcaggttt tcatcctggt aacaggggtt
    31921 cgattcccct atggactaca aaaaaattca atggtattcg ttaccagtat cttctgtttt
    31981 tttattgcct ttttttctaa taatttggtc gttgtctgtt atttgcagcc atgacaaagg
    32041 ataacagtcc tttaatcggg ttcaggcggg attgcatctt ctcatttcaa ctgtttcttt
    32101 atgtagatct gttcaccatg cgaaaaagaa tatttctatc tgttttgatt gcattatgca
    32161 tactttttac cggaagcagt tgctccatga aaagacaaaa acaatcgaat actgttgtaa
    32221 gtattaaaga tgagaaattt ctgatcaacg gcaggcccac ataccaggga ataagctggc
    32281 aggggctccc aatcgagggc cttttattga attcaagaat ggtacaggga atattcgatg
    32341 ataaaaatcc tgtaaccgct aaacgatgga aatatcctga tactcaaaaa tgggatcctg
    32401 acaggaatac cgatgaattc gtaaaagcta tggatgaatg gtatgccagg ggtttgatct
    32461 ccttcaccat taaccttcag ggtggcagcc cggtaggata tggcaggatt gattgtataa
    32521 actcatctat aaagccggat ggcgaacttg acaataaata tatgggccgc attgaaaaaa
    32581 ttctgaatcg ggcgaatgaa aaagggatga tagttattct gggcattttt tattttggtc
    32641 aggatcagaa tataaaggat gaagcagcag tgaaaaaagg agttgaaaat acggttgact
    32701 ggctttttga aaagaattac agaaatgtca taattgaaat aaacaatgaa tgcaatatca
    32761 aagattatga tcatgatatt cttaaacctg aacgtgtgca tgaactgatc tcttttgcta
    32821 aatcaaagat aaaaaatggc tatcggtatc ttgtcggcac cagctatggt ggcggaacag
    32881 taccgcttga aaatgtcgtt gctgtttccg atttcattct tatacacggg aacggagtag
    32941 aagaccccgc aggaattaca gaaatggtaa aagaaacaag agcagtaaaa ggctataaac
    33001 caatgccgat catattcaac gaagacgatc attttgactt tgataaaccc gttaataatt
    33061 ttacagaggc aataaaagta tatgcttcct ggggatattt tgactatcgt atgaaaggtg
    33121 agggttttga cgatggtttc cagtccttac ccgttaattg gagcatcagt tcccaaagaa
    33181 aaaaggcttt ctttgacaaa ctggaagaaa ttaccggagg atttaaaaaa taaaaaagtg
    33241 acgattaaaa aacgataata acataagtgc gtttaaattt tagggtaaat aactgacttt
    33301 aaagagctta acaatacttg cagaaactgc tggtcttgta gtgcaagcag aaaagaaatg
    33361 aaagagtcct ccttggaaag ctgatgctaa ctccaaaaaa ggaattgcat tataaaagtg
    33421 tcaaaaagtc aaaatgcctt aatcctttac gcaacggaca gaaaacccca taaccttaag
    33481 gattggctca taggacactt tggtttcatc ggaggccagg ccaaagatct ttgcggagga
    33541 ggtcgcattc tctgtagcac tccaccagca acctagccag cccatgtaat cgaaaatatt
    33601 gggataacaa cagccacccc cggaagggct gtaaaagtaa ccgcttaatt tgttgcacaa
    33661 ccatttgcag acaatgcaat cttttatagc taataaaaac ctgttatggg tactgcaggt
    33721 atttacaatg ccagcgggct acaggattta aaaatgctcg ctcgtctatg ccgtagcata
    33781 cacgactctt gcggaaaact tttatttttg actcttctgc catcctgtca attgtttcga
    33841 tacattttca actgcctcgt ttattcttac aaatttctca aggcttattt gcttcatatc
    33901 tttcattaca cgtatgagca aacgaattac atctatttgc tctcgtgcta tttgaagtac
    33961 atcggctttt tgatgcctgg tattggcacg atatatcaaa gtaagcagtt ctatcgtttc
    34021 tttcttcaaa ctctcgccta ccgtatattt atattccctg ctaaattctt ttgtaaattg
    34081 gaatatcgcc aaaacaaatc ataagttgcc ttttatacgg gtaattcgtt gtattgcgcc
    34141 aaagttcaaa aaaattcgac cgcctacggc ggtgaataaa caaataaatc aaatagtcaa
    34201 atgaatcaaa taaattctta atcccttaca cagcgaaca
//
LOCUS       DAOSPX010000004        30996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_1617_length_30996_cov_4.446366, whole genome shotgun sequence.
ACCESSION   DAOSPX010000004 DAOSPX010000000
VERSION     DAOSPX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30996
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1617_length_30996_cov_4.446366"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLZ75_00430"
     CDS             <1..415
                     /locus_tag="PLZ75_00430"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOS70893.1"
                     /translation="FLNSLDDTAQLGKTILYNLNPSDNEMLITMAGNFNDGSTAVKVQ
                     FGAAWWFLDQKTGMEKHIRNLASFGLMRRFIGMVTDSRSFLSYPRHEYFRRVVCNFVG
                     EEVEKGNIPDEEEILKPLIEGISYRNAREYFGFGE"
     gene            494..1330
                     /locus_tag="PLZ75_00435"
     CDS             494..1330
                     /locus_tag="PLZ75_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS70894.1"
                     /translation="MDELNLKGKVAVITGGAGVICSEMAKSLAAKGVKTAILDLDGEA
                     ARKIAGRLQNEFGTPCIGVAASVLDKASLEQAKKEINREFGEIDILVNGAGGNSPEAT
                     TKTEIMDGSENPEDTFFGLKTEGFDKVFDLNFKGTLLPSMVFTTDMVKKRSGVIVNIS
                     SMNSYRPLTKIVAYSAAKSAINNFTMWLAVHFARVGIRVNAIAPGFLLTNQNRFLLVD
                     EKTGGSTPRGQKVLNNTPMEEYCIPEELTGTLLYLASDFSKAVTGVVIPVDKGFSAFS
                     GV"
     gene            1348..2619
                     /locus_tag="PLZ75_00440"
     CDS             1348..2619
                     /locus_tag="PLZ75_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOS70895.1"
                     /translation="MLYYQSGSENTIIGPEELQNGLYSALDRLGPKKRVLVIPPDFTR
                     FHSRAGEITSLLYKYYGEKLSDILPALGTHFPMSDSELDTMFPGVPKELFRVHDWRRD
                     VVTAGIVPGEYISEITGGALDYSWPAQLNKLVYNGGHDLILSVGQVVPHEVIGMANYN
                     KNLLVGTGGAEGINKSHFVGAVYGIENILGKADNPVRRMLNYASERYIKHLPVVYIHT
                     VVGRNDSGELVIRGLYVGDDPEVFTIAASLSLKVNFTIFEKPLQKAVVYLDPSEFKST
                     WLGNKSIYRTRMAMADNGELIVLAPGLKEFGEDREIDRLIRKYGYRGTPATLKALETS
                     EELKENLSAAAHLIHGSTEGRFSVTYCPGFLTREQIESVNFRYADLGEMLKKYDPGKL
                     RDGFNTTGEGEEFFYISNPATGLWSSMERLN"
     gene            2635..3621
                     /locus_tag="PLZ75_00445"
     CDS             2635..3621
                     /locus_tag="PLZ75_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS70896.1"
                     /translation="MINWGIIGCGNVTEVKSGPAFSKVGGSRLAAVMRRNAELAEDYA
                     ARHKVPKVYTDASELIKDPEINAVYVATPPGNHAEYAIMSIKAGKPVYIEKPMARNYG
                     ECLSIIEASEKHRVPVFVAYYRRTLPGFLLVRDLIAKGAIGKVRHVLIRCFKSPSQEE
                     LTGKLPWRVMPEISGGGHFFDLASHQLDYLDFLLGPVVKVKSVVINQAGLYRAEDFVS
                     VSFLFPDNIACTGIWCFNASPQSNSDVIEITGESGRIRFSTFSFEPVVMENNEGRKEF
                     VNERPEHVQFYLIEQIVRELSGGSKAVSTGTTAARTNRVMDEVVAEYYRTFA"
     gene            3675..5129
                     /gene="gnd"
                     /locus_tag="PLZ75_00450"
     CDS             3675..5129
                     /gene="gnd"
                     /locus_tag="PLZ75_00450"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOS70897.1"
                     /translation="MDKLSDIGLIGLAVMGENLVLNMESKGFRVSVYNRTTQKVDDFL
                     AGRARGKNIVGTHSVEELVKSLARPRKIMIMVKAGSAVDDMINTLIPLLDKGDIIIDG
                     GNTHFPDTNRRTALAESKGLLYIGTGVSGGEEGALLGPSIMPGGSKAAWPFVKPIFQA
                     IAAKVEDGSPCCDWVGEDGAGHFVKMVHNGIEYGDMQLICEAYQIMKDLLGMSAGEMH
                     EVFREWNEGELDSYLIEITRDILAFKDEDGEPLVDKILDTAGQKGTGKWTAVSALDLG
                     IPLTLIGEAVFSRFLSAIKEERVKASGILNGPVPAFRGNREEFINDLRDALYASKIVS
                     YAQGYSLMRAAALEYKWDLNYGGIALMWRGGCIIRSVFLGKIKEAFDNNPSLDNLLLD
                     PFFRTKLEKSQKGWRKVVSDALLNGIPVPAISSALGYFDGYRCERLPANLLQAQRDYF
                     GAHTYERIDRPRGEFFHTNWTGRGGTTASTAYNA"
     gene            5150..6448
                     /locus_tag="PLZ75_00455"
     CDS             5150..6448
                     /locus_tag="PLZ75_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS70898.1"
                     /translation="MSEKVKIGNYRWTVCALIFFATTVNYIDRQVIGILKPLLETDLN
                     IGEKEYSYIVMAFQLFYALGLVLAGRLIDKIGTKIGYAIAVTLWSIAAMGHALAKGAL
                     GFGFWRGLLGVSEGANFPAAIKTVAEWFPKKERALATGIFNSGTNVGAIVAPVAVPAI
                     VVLWGWQAAFIITGALGFIWLIFWIIYYEIPERQKRVKASEIAYINSDVEEQAEPKSD
                     VPWLKLLKYRQTWVFFIGKGLTDPIWWFYLFWIPGWLAEVRGTGLDLKSFGLPLVFIY
                     SMTTVGSIFGGWLSGYMIKKGMTPFNARKYSMLIFALLVTPIVFAQAPGISTWGAVML
                     IALAASSHQAWSANIFTTVSDAFPKRAVSSVTGIGGMAGALGGLGISYLAGAVLDHYK
                     NAGHVETGYVIMFVVAGCAYIVAWAIMNIFAPKNKTVTLD"
     gene            6449..7702
                     /locus_tag="PLZ75_00460"
     CDS             6449..7702
                     /locus_tag="PLZ75_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOS70899.1"
                     /translation="MKKLGKYSFGMGDRFGHQGSAQLAAIIEAEKKGVDITPVWNKSN
                     REHMIIGTRPEDVRKEADEATRKAGFTRPYFVDADHINLDNVDKFIGSSDFFTIDVAS
                     YIGRKTDEESIRKFVSGCSGYTGELKIDGLKPYPVVSESQILSFAEKYLFAAQKAGEI
                     YRKIEAQKGRDNFVAEVSMDEVPSPQTPVELFFILKMLGEEQIPAQTIAPKFSGRFNK
                     GVDYVGDPLRFAAEFEADLMVIDMAVKEFGLPGNLKMSVHSGSDKFAIYPHIGSLIRK
                     HDKGLHLKTAGTTWLEEVIGLAESGGEALEFVREIYSDALTDIEELCAPYADVIDIDA
                     GALPSSGEVAGWDSSRFAGALRHVPGNPDYNPDMRQLIHVGYKLAAMRMDEYFRLLEA
                     NEKTVSACVFENMYNRHICRLFGLE"
     gene            complement(7812..8675)
                     /locus_tag="PLZ75_00465"
     CDS             complement(7812..8675)
                     /locus_tag="PLZ75_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOS70900.1"
                     /translation="MKRKISLIAALFALPALILASEGGLYGSGIILPERGLCAHRGAM
                     ATHPENTIPAFREAVIAGAHMIEFDVFLTRDNHMVVIHDETVDRTTNGSGKVSDLTLE
                     EIKSLDAGSWKSAEFAGAEIPTLDEVLAVMPFNVWLNIHLKGEGRLPVMVAEKIASEN
                     RLHQAFLACSRKAAEMAREAVQGIMICNMDRQNDNLNYINETIESEAEFIQLTQPITP
                     SLPDYTRRLKEKGIRVNFFGTDSPEEIRVLFKSGVDFPLVNDIIHLMKIAGETGIPPV
                     VPQFRAGIQKK"
     gene            complement(8798..10615)
                     /locus_tag="PLZ75_00470"
     CDS             complement(8798..10615)
                     /locus_tag="PLZ75_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS70901.1"
                     /translation="MIRLKIYFLLFALILAGTTVPAQKAKHTFRLSPTEFLLDDEPFQ
                     IISGELHPSRIPAEYWQHRIRMARAMGCNTISVYIFWNYHESEEGVFDFSTGNRNLAE
                     FIKLVQKEKMWLIVRPGPYVCAEWELGGIPPYLLRIPDIRLRCMDPRYMAAAERYMSR
                     LADEIRPYLITRGGPVIMLQIENEYGSYANDRNYTQRLKEVWQANGIDVPTFTGDGPT
                     TYMLEAGTLPGSAVGLDPGSTAADFELAAKMNPGVPVFSSETYPGWLTHWGEEWNRPD
                     TVRLLEEVKFLMDNRKSFNFYVIHGGTNFGFTAGANSGRKGYEPDVTSYDYDAPINEQ
                     GRPTAKYFALRNLIGSYLPKNEKLPKIPDTIPAITIPLIFPTKFTSIWDNLPEPVSSV
                     TPNTFEAYGQDYGFILYRTRLIGHKKGWLTITDIHDYATVFLNGEYIGHLDRREGINT
                     IELPESKADVPVLEILVEAMGRINFAQHLIDRKGITERVTLNGMTLMNWEIFKLPMDW
                     KYIYDLRSSGKVINKPGVFFKGNFSLAITGDTYFDVSNYKKGIVWVNGHNLGRYWDIG
                     PQKRLYCPASFIRQGLNEIIILDLHMTDPKPVIAVRTLE"
     gene            complement(10745..12565)
                     /locus_tag="PLZ75_00475"
     CDS             complement(10745..12565)
                     /locus_tag="PLZ75_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS70902.1"
                     /translation="MNKIKLLLKYIAPYKWQAFNSVVFNILSAVFALVSYTLAIPFLK
                     IMFNRVDVLQHPGDFQLSIDYFNSASRYFLSLFIDRHGQAGALLIVSVVFVIASLLKN
                     GFIFLANNSMAYIRSYTVRDLRKKMFDKVLKLPLSYFSDARKGDLMTRISNDVQEIEV
                     SVMSSLTMLFRDPMYILIFVIYLFVSSWKLSLYALVLLPVSGWFIGRVSRTLRSSSLV
                     QQQQLGRLLSVVEETLTGLRIVKGFNAEKKMQEQFRRINERYSKIFRRVIRKYYLASP
                     LSEFLSTIVVMALLYLGGMMALSGETNMTPDKLIAYLIVFSQIIPPAKNITTAWFSIQ
                     KGMASIERVDQILEAEEKIRERIDPVPVKEFREEIEFRGVWYSYNTEPVLKDINLTIR
                     KGQTVAIVGKSGAGKSTLADLLPRFIDADKGSVLIDGVDVRDMNITDLRNLMGIVSQH
                     PILFNNSFYENIAFGMNSSLQEDVENAARIANAHDFIMETESGYENNVGESGNKLSGG
                     QKQRISIARAILNNPPILILDEATSALDTESERLVQDAILRLMENRTSIVIAHRLSTI
                     QHADLIVVIDDGMIVERGTHAELMNRKDGFYAKLRNYQAI"
     gene            complement(12584..13927)
                     /gene="purB"
                     /locus_tag="PLZ75_00480"
     CDS             complement(12584..13927)
                     /gene="purB"
                     /locus_tag="PLZ75_00480"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOS70903.1"
                     /translation="MDLNSLTAISPIDGRYRQKVNELDLYFSEFGLMRYRLMVEIKYF
                     ISLCDIPLPPLADFRKDNYNVLRSFYEDFSMADAERIKENERVTNHDVKSVEYFLKER
                     FRDSGWLKWSEFIHFGLTSQDVNNTAIPLSLKDAMINVYFPLLYDVRDMLAGLADEWK
                     NIPMLARTHGQPASPTRLGKEISVFVTRIDEQSKYFGQIPYSAKFGGASGNLNAHRAA
                     YPDIDWILFADNFVNGELGLQRSFPTTQVEHYDNLAALFDNMRRINTVLIDLSRDIWT
                     YVSMNYFSQKTRKGEVGSSAMPHKINPIDFENAEGNLGYANAIFGHLAEKLPLSRLQR
                     DLTDSTVLRNLGVPLGHTLVAFSSLLKGLNKLVVNHEEIKNDLENNWIVISEAIQTIL
                     RREGYPQPYEILLELTRGNVKISREAVMDFINALEISEAVKEELKALTPFNFTGY"
     gene            14053..14817
                     /locus_tag="PLZ75_00485"
     CDS             14053..14817
                     /locus_tag="PLZ75_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOS70904.1"
                     /translation="MTPEKIRISAVRYANTYPFIYGLTETGFDRKVILSTDHPADCAA
                     RLAAGKADIGLVPVASLPLISNYQIITDYCLGAYGKVRTVMLLSNCTFGEIRNIFLDY
                     RSRSSVNLARILAKHWWKREFGWKNTSENFDFKNIPEKDGIVLIGDQCFELGKQFRYG
                     TDLAEEWYDFTGLPFAFACWTANRQLPQAFLKEFNEALATGVDNIPEVVRKYGRKGII
                     RGKALETYLTENISFNLNEDKRKAIKLFLNYLKKSD"
     gene            14941..15186
                     /locus_tag="PLZ75_00490"
     CDS             14941..15186
                     /locus_tag="PLZ75_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOS70905.1"
                     /translation="MAVTITFNELRKIKDLLPPGSIRKIADKLDMNEETVRNYFGGRN
                     FEKGESVGVHFEQGPGGGIVSFDDSTILEMAKKMITE"
     gene            15302..15961
                     /locus_tag="PLZ75_00495"
     CDS             15302..15961
                     /locus_tag="PLZ75_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOS70906.1"
                     /translation="MNLSAIISQEEEKSLTALEGFFVLKWGGTNLPSHDLQHHRRVWT
                     NIKEILNHCNDPDIDRARISNLLMAAYLHDIGMAREQGVRHGFHGKKAAEEYISANRL
                     KRKDYEEALNAIEFHDNKNYDNDSGNNPVLKILSVADDLDAFGTTGIWRYMEIYRARG
                     IPEEIIGSRILENAASRFENFKAFSSDKPLLFQKHRERYNVLKRHFGNYNQSMAEAGD
                     K"
     gene            complement(16036..17112)
                     /gene="dinB"
                     /locus_tag="PLZ75_00500"
     CDS             complement(16036..17112)
                     /gene="dinB"
                     /locus_tag="PLZ75_00500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOS70907.1"
                     /translation="MRKIIHIDMDAFFASVEQLDNPELRGKPVAVGGSGERHVVAAAS
                     YEARKFGVRSAMPSITARRLCPELIFVRHRFDRYNEVSGQVFSIFSDYTDLVEPLSID
                     EAFLDVTRDRKNIGSATIIAGEIKKEIRQKTGLTASAGVSFNKFLAKMASDIKKPDGL
                     FLISPQEAEKFIEKLPVEKFYGIGKVTAEKMHKLGIHNGADLKKPDLAFLVRNFGKAG
                     KFYYDIARGIDDRPVLAESERKSIGTEITYEKDLTTQFEIITELYKVEMELMQRVEKA
                     GTTGRTVTLKIKFSDFRQITRSKTFPHFIRDFPALHRAVTEIRKSLSLDNVRIRLLGV
                     TISNLESEDCEDQQLMLFNEEDCC"
     gene            17325..20138
                     /locus_tag="PLZ75_00505"
     CDS             17325..20138
                     /locus_tag="PLZ75_00505"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOS70908.1"
                     /translation="MSESLLPYPSDDTLKILENEEIELVRITNDYSDKQIFRIYSREK
                     NVKDFLENVKPEITGKYIRPYIERRISRCFFIIRDEGTLCFQRREQSNILYPEDVLEI
                     IPDSAIPVFRFDRGKEFTTYQLTVESEGEKIELCRKAVDILCNNPCIIRHGNKIFQIS
                     GIDGQKLRPFLEKESVIIPKNSELKYYSGFVLNVVNNCTAEGKGFKIIYPESEKWPGL
                     SLENSIMGYPVLILSYNYSGITISPGDNNEYFTVFAHENGEFIYRKYKRDFRWELQCR
                     EILSEIGFNSEDKINFSLQDENLKPVSQINKVIEAVNRNYNHLVSQGFLISSKHLDKN
                     YNLRNISIEIFNKSEGDWFDLDAVIKIGEWDFSFIRFRNHILNGNREFELPDGTTAIL
                     PEEWLVRYKNIFEFGKDHGDSIRIHKQHFPLLTEVLQNREDESFRNLEKLLLPEKLPV
                     PEVPAGLNCRLRDYQLEGLAWLQWLQSSRLGGCLADDMGLGKTIQALAMLQMNKETSD
                     KPGKSATVHEPTLFDDPPVNLTSLIVVPPTIMHNWENEIRKFVPQMNIYIHRGYRRLR
                     SCSDFIKHDIILSSYHTVKQDIDLLSSFHFHYIILDESQYIKNPASLAYKCMIRLKSD
                     YRLVLTGTPIENSVTDLWAQLNFVNRGMLGSLTWFRNTYARPIEKDRDDLTENRLKKH
                     IKPFILRRTKEMVAADLPPVSQQLIFCEMTVEQQKIYESEKSAVRNVILEGQAAGKKG
                     KTAIIILQGLMKLRQISNHPVLTDEDYSYSSGKFEAVMNDIENVVNEGHKILVFSSFV
                     MHLKLFEEALGKKQIKYTIMTGASKDREKLIGSFQNDPSSRVFLISLKTGGVGLNLTS
                     ADYVFILDPWWNPAAEQQAVSRAHRIGQDKNVFIYRYISSGTLEEKITKLQERKSKLA
                     GTFIATTNPLRDMDMHEILDIIE"
     gene            complement(20157..21938)
                     /locus_tag="PLZ75_00510"
     CDS             complement(20157..21938)
                     /locus_tag="PLZ75_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOS70909.1"
                     /translation="MINLSINNQKISVEEGTTILDAARKLNINIPTLCNHPDLCVAGN
                     CRVCVVEQTGARTLIAACAMPVAEGMDIHTNTLKVRNARKHIVELLLSEHRSDCTKCY
                     RNQNCELQSLANEFAFGDTIFLDLVEDHPYVIDRSSPSFIKDDSKCIRCQRCVRTCTE
                     LQHISAIAVANKGMHQKISSFHDKPMSHVVCTNCGQCVNRCPTGALVEKTYIDQVWDA
                     IYDPGKHVIVQTAPAVRVALGEDLGYDPGERVTGKMVTALRQLGFDSVLDTDFSADLT
                     IMEEGTEFLTRLKKVLVDGDKNTALPMFTSCSPGWIKFIEHKYPELLNNLSTCKSPQQ
                     MFGALAKTYYAQKKNLDPSTIVSVSIMPCTAKKYEADRPEMRSSGYKDIDYVLTTREL
                     VIMVKQAGIDFRNLEPSGFDSVMGESTGAAVIFGATGGVMEAALRTAYEIVTGREVPF
                     KNLNITPVRGMEGIKEASIKIEDCVDDWKFLEGAELKVAIAHGLTNANKLMRLVRSGE
                     ASYHFIEIMACPGGCLGGGGQPIPTNAEVRKRRMNAIYSEDEHMEFRKSHDNPDVIAI
                     YEEFLGQPNSERAHELLHTHYTEREAY"
     gene            complement(21960..23120)
                     /locus_tag="PLZ75_00515"
     CDS             complement(21960..23120)
                     /locus_tag="PLZ75_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOS70910.1"
                     /translation="MKKRGLNKVDLIFEKVDLREVLTVAYNMPQAEIIQKVLDSGLKG
                     RGGAGFPTGLKWKYTAAEKDPDKYIVCNADEGEPGTFKDREILDRVAYKVYGGMVIAG
                     KAVGAKTGIVYLRGEYKFLLPQLLAELDEFHRLIKEIGFDFSISYRMGSGAYICGEES
                     ALFESIEGFRGEPRNKPPYPTVSGVFGKPTSINNVETLVTALMVIKHGPESYSQLGTK
                     DSRGSKVFSISGDTPTPGIFELELGMTVQAFVNEFGDGDTKAVQVGGASGFCVPRKRF
                     EDTIIGFEGVPTGGSMKLFNSSRSMYNVLHNYLDFFAEESCGQCTPCRVGCQQLLKGV
                     QKVKKGEVKSTYLTELIKLADTMKVASKCGLGQSVGNAFKTIVENFREEIIY"
     gene            complement(23133..23600)
                     /locus_tag="PLZ75_00520"
     CDS             complement(23133..23600)
                     /locus_tag="PLZ75_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOS70911.1"
                     /translation="MYQLGNLVKELTEKHGRSRESLMPILQGVVERHSYLTDEAMVEI
                     ANQLDISAAEVYGTASFYTFLDTKERGKYVIRVCKTITCAMKGKNEILETLEDMLKVK
                     VGETTADKQFSLLETNCIGWCHKAPAMLINEMPYTELTPEKVVEIIKSYMQKK"
     gene            23986..24405
                     /locus_tag="PLZ75_00525"
     CDS             23986..24405
                     /locus_tag="PLZ75_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70912.1"
                     /translation="MAVEFLCKVCRGHLKPKTSIILTATNLKNRTEKGLVFLNPEIGN
                     YTHTTHSSFQIRKGEEYIFTCPICNAQLNSMKYLHLVRILMIDEGGNESSIYFSGIGG
                     EKCTYKIRDDKVEASTGPDVKIYNKYFDVPEEDRKYL"
     gene            24486..26528
                     /locus_tag="PLZ75_00530"
     CDS             24486..26528
                     /locus_tag="PLZ75_00530"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOS70913.1"
                     /translation="MNTDVNKLAADNIRILSMAMVEKAKSGHPGGAMGGADFIHILFS
                     EFLRFDPDDSSWINRDRFFLDPGHMSPMLYSVLTLTGLFSIDDLKQFRQWGSPTPGHP
                     ELDVARGVENTSGPLGQGHTMAVGAAIAERFLAARFGELFAHKTYAFISDGGIQEEIS
                     QGAGRLAGHLGLNNLIMFYDSNDIQLSTETSAVSTEDTAAKYMAWGWEVVTIDGNDHD
                     QIRKALSQARESKDKPFLIIGRTIMGKGLIDNEGISFERKISTHGMPVSDAGASFEKS
                     LAGLGGNLSDPFTIFPAVKEYYAAVIEEKRRYATDWKRQKKEWAAENSDSERKLNLFY
                     SGDIPAINFESISQKEGIATRAASAAVLGVLANTIENMVVASADLSNSDKTDGFLKNT
                     KPLVKGDFSGAFLQTGVSELTMAAVMAGMALHGGIIPACGTFFVFSDYMKPAVRLACL
                     MGLPVRFIWTHDAFRVGEDGPTHQPVEQEAQIRLMEHLKNHKGENSMLVLRPADALET
                     NVAWKLALENKKTPTALILSRQNIRDLPSSTGSRYRDALMMSRGAYTVSDCKGRPDII
                     LVASGSEVATLVEGAELLKKDGLRVRIVSAPSEGLFRNQDQSYRESVIPDDVPVFGLT
                     AGLPVTLQGLAGPRGKVWGVNSFGYSAPYKVLDEKLGFTAGNVYRQVKDYLLHYKK"
     gene            26666..27736
                     /locus_tag="PLZ75_00535"
     CDS             26666..27736
                     /locus_tag="PLZ75_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70914.1"
                     /translation="MKRILFISALMLAMGINSSFSQNDSIRKEYAVRDTISEDIILFD
                     SDELLDIELRFNITQYRRKRDKSEYLDATLTYHVSQDDSIIKYLKIRPRGISRLTICD
                     FPPLLLNFKKNDAVPGEFNRVDKIKMVTHCSAGGEDNLLREFLVYKMYNVLTDYSFRV
                     RLLRVNYVNSEKPDKKQVREFAFLIEPVKSLGKRTNSVEVETAHVTQLHIKREIMDRM
                     AIFNYMIGNTDWSVPTRHNTLTMTEGSYGGLGSAIIVPYDFDYAGVVNTHYAVPFEGL
                     GLESVRQRRYLGVCRTEQEFSEALVEFMEKKEEIYRVINDFPYLSGKSKKDMINYLEG
                     FYRSLDKRNSIINSLRRDCIII"
     gene            27757..28362
                     /locus_tag="PLZ75_00540"
     CDS             27757..28362
                     /locus_tag="PLZ75_00540"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOS70915.1"
                     /translation="MSFESIATETTLLNIMIRFAVNLLVLFILIRFIYYRFTRKEEYV
                     FSLFMMGIIIFLLISLLGTVNIQLGMALGLFAIFAILRFRTVNYTAKDMTYVFTVIGV
                     SIINSQANIPPPVIGALIVNTTILVSAVILELFLKKRALSTMIVNFSKPELLRPERRK
                     QLLEELSAQTGQEIVKISVRKIDVNKGSIELEAFYADKIAD"
     gene            28445..29044
                     /locus_tag="PLZ75_00545"
     CDS             28445..29044
                     /locus_tag="PLZ75_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOS70916.1"
                     /translation="MQYDIQQGIKGLVFDLDGTLADTMPYHFQGWKKACEKYGARIDN
                     HFLRKYTGSPSWIVAGEVLKHCGLNNSVTIEEIMNEKHREFFRLQHLVKPITPVTEIV
                     KKYYGKLPMAVGTGGHREAVERTLEITGLREYFDIVVTANDVNAFKPDPETFLKCAEL
                     MNVEPRDIEVFEDAELGIRAAISAGMKVTDVRSWYNSDW"
     gene            29352..30839
                     /locus_tag="PLZ75_00550"
     CDS             29352..30839
                     /locus_tag="PLZ75_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS70917.1"
                     /translation="MKLDRRHFLKLSSIAGTGIVGSGILQSCKPGSGGKAVSALDLIK
                     EQSLAKHPQKFNMCGYAAPALDVVRTAFIGLRRGSTHLTSVSLLEGVEIKALCDLVPG
                     VVDNAMRRIEHTGFKPDTYTGNPEAWKKMCERDDIDLVYIATPWDMHTPMAVYAMEHG
                     KHVCIEVPAAVTMDECWQLVEVSESTQKHCMMLENCCYDFFEILTLNMARQGFFGEIV
                     HGEGAYIHTLLDSNFSKTGYWDMWRLRENSSRQGNLYPTHGLGPICQIMDINRGDKME
                     YLTAAMSNDFQMNETARKLAAVDDFYSEFVDRPYRGNMTTCTIKTNKGRTIMLQHDVT
                     SPNVYSRIHKVSGTIAAALKYPLPPKISVGHQRWLNEEEFKEVEEKYTPEIIKRIGEM
                     AKQVGGHGGMDFLMNWRLIDCLRNGLPFDQDVYDAALWSAVGPLSEWSAANRSNSIDV
                     PDFTNGAWKTNRPVDLLLEQGGNTRVIRSNAPPRRRPQPVAEGVE"
BASE COUNT         8865 a         6776 c         6958 g         8397 t
ORIGIN      
        1 ctttctcaac agtctggatg atactgcaca actgggcaag acaattcttt acaatctcaa
       61 tccctctgac aatgaaatgc tgataaccat ggcaggaaat ttcaacgacg gcagtacagc
      121 cgtgaaagtt cagttcgggg ctgcctggtg gttccttgat cagaaaacgg gtatggaaaa
      181 acatatcagg aacctggcct cattcgggct gatgagaagg tttatcggca tggtaaccga
      241 ttcaagaagc tttctgtcat atcccagaca tgaatatttc aggcgtgtgg tatgcaattt
      301 tgtgggtgaa gaagttgaaa aaggcaatat acctgacgaa gaagaaatac ttaaacctct
      361 cattgaaggt atttcttaca ggaacgccag ggaatatttc ggattcgggg aataaacccg
      421 taccggctgt atccggtctc tgctcatcag aagaaaataa acaacaaggg aaatatgata
      481 aattattaaa aatatggacg aacttaattt aaaaggtaaa gtggcagtaa tcaccggggg
      541 tgccggggtg atatgctctg aaatggctaa atcacttgct gccaaaggtg taaaaacagc
      601 tattcttgat cttgacgggg aagctgcaag gaagattgcc ggcaggctgc agaatgaatt
      661 cgggacgcca tgcattggcg ttgcggcaag tgttcttgat aaagcttcac tggagcaggc
      721 aaagaaagag ataaaccggg aattcggaga aatagacata ctcgtgaacg gagccggggg
      781 aaactcacct gaagcaacaa caaaaacaga aataatggat ggctcggaaa accccgaaga
      841 cacatttttc ggtcttaaga ccgagggttt cgataaagtt tttgatctga acttcaaggg
      901 cactctcctt ccgtcaatgg tttttacaac agatatggta aaaaaacgct ccggtgtaat
      961 agtcaatatc tcctcaatga attcctacag gccgcttaca aagattgttg cctattctgc
     1021 cgccaaatcg gccataaata acttcacaat gtggctggcg gttcattttg caagagttgg
     1081 cataagggta aatgcaattg ctccgggttt tcttcttaca aaccagaacc ggtttctgct
     1141 ggttgacgaa aaaacaggag gatcaacacc cagggggcag aaagtattga acaatacacc
     1201 catggaggaa tattgcatcc cggaggaact taccggtacc cttttatatc ttgcttctga
     1261 cttttcaaag gcagttaccg gtgtggtaat tcccgtggat aaaggattca gtgctttcag
     1321 tggtgtgtag attaaattat tcattcaatg ttatattatc agtcaggttc ggaaaatacg
     1381 ataataggtc cggaggaact tcagaacggt ctttactcgg cactggacag gctcggacca
     1441 aaaaaaagag tgctggtgat accgccggat ttcacccgct ttcactccag ggcaggagaa
     1501 attacaagcc tcctttataa gtattacggg gaaaagctca gtgatattct tcctgcactg
     1561 ggcacacatt ttcccatgag cgacagtgaa ctggacacaa tgttcccggg agtaccgaag
     1621 gaactctttc gtgtacacga ctggagaagg gatgtagtca cagccggaat cgttccgggg
     1681 gaatatattt cggaaattac cggaggtgcc cttgactatt catggccggc ccagctcaac
     1741 aagctggtct acaacggcgg tcatgacctg atattgtcag tcggacaggt ggtgccgcat
     1801 gaggttattg gcatggccaa ttacaataaa aaccttctgg tcgggacagg tggtgccgaa
     1861 ggtatcaata aaagccattt tgtgggagcc gtgtacggaa ttgaaaatat tctcggaaaa
     1921 gccgacaacc cggtgagaag aatgctgaac tacgcgtcag aacgttatat caagcatctg
     1981 cctgtagtgt atattcatac ggtggtcggc agaaatgatt cgggagaact tgtaatacgc
     2041 ggcctctatg tgggcgatga tccggaagtg ttcaccattg ccgcttccct ctcgctcaag
     2101 gtcaatttta caatttttga aaaacccctg cagaaagctg ttgtttatct tgatccatct
     2161 gaattcaaga gcacctggct cggaaataaa agtatataca ggacaagaat ggccatggca
     2221 gacaacggtg aactcattgt gcttgctccc ggacttaagg aattcggtga agacagggaa
     2281 attgaccgtc ttatcaggaa atacggctac aggggaacac ccgcaacact caaagccctg
     2341 gagaccagcg aggagcttaa ggaaaacctc agtgctgcag cccatcttat tcacggaagc
     2401 actgaaggaa ggttttccgt tacctattgt ccgggattcc tgacgaggga acagatagaa
     2461 tcggttaatt tcagatatgc cgacctcggc gaaatgttga aaaaatatga tcccggaaag
     2521 ctcagggacg gtttcaacac aaccggggaa ggcgaggaat tcttctacat ctccaatcct
     2581 gccaccggct tgtggagctc catggaaaga ttgaattaac cgtaaaccga ggggatgatc
     2641 aactggggca taattggctg cggcaatgtt acggaagtga aaagcggacc ggctttcagc
     2701 aaagtcggcg gatcccggct tgcggcggta atgaggcgga atgcggaact ggcagaggat
     2761 tatgccgcga ggcataaggt gccgaaagtg tataccgacg cttcagaact gataaaggat
     2821 cctgaaataa atgccgtgta tgtggctact ccgccgggaa accatgccga gtatgcaata
     2881 atgtcaataa aggccggcaa gccggtttat attgaaaaac cgatggcccg gaactatgga
     2941 gaatgcctca gtattatcga agcatcggaa aaacacaggg tcccggtatt tgtcgcttat
     3001 taccgcagaa ccctcccggg attcctgctg gtgagggatc tgatagcaaa gggggcaatt
     3061 ggaaaagtga gacatgtgct gatccggtgt ttcaaatcac cctcacagga agaattgaca
     3121 ggaaaactgc cctggagagt catgcctgaa atctcaggag gaggccattt ttttgacctg
     3181 gcatcccatc agcttgatta tctcgatttt cttctgggtc cggtggtgaa agtgaaatca
     3241 gtcgttatca accaggccgg cctttacagg gctgaggatt ttgtttccgt ttccttcctc
     3301 tttcctgaca atattgcatg cacgggcata tggtgcttca atgcatcgcc gcagagcaac
     3361 agtgatgtta ttgagattac gggagaatca ggcaggataa gattttccac cttcagtttc
     3421 gaacctgtgg tcatggaaaa caatgaaggc cggaaagaat tcgtgaatga aagacccgaa
     3481 catgttcagt tttatcttat agaacagatt gtaagggagc tttcaggcgg aagcaaagca
     3541 gtatcgacag gtacaacggc agccaggaca aacagggtaa tggatgaagt agtggcagaa
     3601 tattacagga catttgctta aacagcagca cactgttaca ggatatttac ttaattaacg
     3661 ataaacttat tattatggat aaactttcgg atattggatt aataggcctt gcggtaatgg
     3721 gtgagaacct ggtactgaat atggaaagca agggatttag ggtttccgta tacaaccgca
     3781 cgacacagaa ggttgatgat tttctggcag gaagggccag gggtaaaaat attgtcggga
     3841 cacactctgt tgaggaactt gtgaaatccc tggcacgccc caggaaaatc atgatcatgg
     3901 tcaaggccgg ttctgcggtt gatgatatga tcaatacact catacctctg cttgacaagg
     3961 gagatatcat cattgacggc ggaaacactc atttccccga taccaaccgg cgtaccgccc
     4021 ttgctgaaag caaaggactg ctctatatcg gcaccggggt atcaggcggc gaagaaggag
     4081 ctctcctcgg accatccatc atgcctggcg gatcaaaggc agcatggccc tttgtcaaac
     4141 ctatttttca ggcaatcgca gcaaaggttg aagacggctc tccctgctgt gactgggttg
     4201 gtgaggacgg agccggacat tttgtaaaaa tggtgcataa cgggattgaa tatggtgata
     4261 tgcagcttat ttgtgaagca taccagataa tgaaggatct ccttggaatg agtgccggtg
     4321 aaatgcatga ggttttcagg gaatggaacg aaggtgagct tgacagttat ctgattgaga
     4381 taaccaggga tattctggct ttcaaggatg aggacggtga acctcttgta gacaagattc
     4441 tcgacactgc cggccagaaa ggaacaggaa aatggacagc agtatccgca cttgatctgg
     4501 gaatccccct tactcttata ggcgaggcag tcttctccag gtttctcagc gccatcaagg
     4561 aagaacgggt aaaggcgtcc gggatactga acggcccggt tccggctttc cggggaaaca
     4621 gggaggaatt catcaacgac ctgagggatg ccctttatgc atcaaaaata gtttcgtatg
     4681 cccagggata ttcactgatg agggctgctg cccttgaata taaatgggat cttaattatg
     4741 gcggaatagc cctgatgtgg cgcggcgggt gcataatacg gtcggttttc cttggaaaga
     4801 tcaaggaagc cttcgataat aacccttcac tggacaatct ccttcttgac ccgttcttca
     4861 ggacaaaact ggagaaatcc cagaaaggat ggaggaaggt tgtttcagat gccctgttaa
     4921 acggcattcc tgtcccggca atttcatcgg ctctcggata tttcgacggc tacaggtgtg
     4981 aacggctgcc tgccaacctt cttcaggctc agagggatta tttcggggca cacacctatg
     5041 aacgtattga caggccgaga ggtgaatttt tccatacgaa ctggaccgga agaggcggaa
     5101 caacggcatc aactgcttat aatgcataag aatttttaat aaaacaatca tgtcagaaaa
     5161 agtaaaaatc ggaaactaca gatggacagt ctgtgcactg atattctttg ccaccacggt
     5221 aaactatatc gacagacagg tcattggaat tctcaagcct ttgcttgaaa ctgatcttaa
     5281 tatcggtgaa aaggaatatt catacatagt aatggccttt cagcttttct atgcccttgg
     5341 cctggttctt gccggaaggc ttatcgacaa aatcgggaca aagatcggtt atgccatagc
     5401 tgtcactctc tggagtattg cagctatggg ccatgcactt gccaaagggg cccttggttt
     5461 cggattctgg aggggattac tgggtgtcag tgaaggagcc aactttccgg cagcaattaa
     5521 aaccgtggct gaatggttcc ctaagaaaga aagggcactt gctacgggaa tctttaattc
     5581 cggaaccaat gtgggtgcaa tcgttgcacc tgttgcagtt ccggcaattg tcgtcctctg
     5641 gggctggcag gcagccttta taatcaccgg tgccctgggg ttcatttggt tgattttctg
     5701 gatcatctat tacgagatac ctgaaaggca gaaaagagtg aaagccagtg agattgccta
     5761 catcaacagt gatgtggagg aacaggccga accaaaatct gatgtaccct ggcttaaact
     5821 cctgaaatac cggcagacgt gggtgttttt cataggaaaa gggctgactg accccatatg
     5881 gtggttttat ctgttctgga tcccgggatg gcttgcagaa gtaagaggta cagggcttga
     5941 cctcaaatca ttcggactgc cgcttgtatt catatattcc atgactacgg ttggaagcat
     6001 attcggaggc tggctctcag gctatatgat caaaaaaggc atgacaccct tcaatgcaag
     6061 gaaatattcc atgctgatct ttgccctgct tgtaacacct atagtctttg cacaggctcc
     6121 cggcatcagc acctggggtg cggtaatgct tatagccctc gcagcatcat cccatcaggc
     6181 atggtcagca aacatattca caaccgtttc ggatgccttt ccaaaaagag cagtcagttc
     6241 ggttacaggt atcggaggaa tggccggtgc gctgggcggc cttggtatct cataccttgc
     6301 aggggcagta ctcgatcatt acaaaaatgc cggtcatgtt gaaacaggat acgtgatcat
     6361 gttcgttgtt gcaggttgtg catatatcgt tgcatgggca ataatgaata tttttgcacc
     6421 gaaaaacaaa accgttactc ttgattaaat gaagaaacta ggcaaatatt cattcgggat
     6481 gggcgacagg ttcggtcatc agggcagtgc acagcttgcc gcaataattg aagcggaaaa
     6541 gaagggtgtt gatattactc cggtctggaa taagtcaaac agggaacaca tgattatcgg
     6601 cacccggccc gaagatgtca ggaaggaagc tgacgaagca acacggaagg caggttttac
     6661 caggccgtat ttcgttgatg ccgaccatat caatctcgat aacgttgaca aattcatagg
     6721 aagttcagat tttttcacaa ttgatgtggc ttcctatatt ggcagaaaga cagatgaaga
     6781 atcaataagg aaatttgttt cgggctgttc ggggtatacc ggagaactga aaatagacgg
     6841 actgaaaccc taccccgtcg tatcggaatc acagatactt tcttttgcgg aaaaatatct
     6901 ttttgctgca cagaaagcag gcgaaattta caggaagata gaggctcaga aaggaagaga
     6961 caattttgtt gctgaagtat caatggatga ggtcccctca ccacagacac ccgtcgagct
     7021 ttttttcatt ctcaaaatgc ttggtgaaga gcagataccg gcacagacca tcgccccgaa
     7081 attttccgga cgcttcaaca agggagttga ttatgttggt gatccccttc gttttgccgc
     7141 tgagttcgag gctgatctca tggtaatcga catggctgta aaagaattcg gcctgcccgg
     7201 caacctcaag atgagcgtgc attcagggtc agataagttt gcaatatatc ctcacatagg
     7261 atcgcttatc agaaagcatg acaagggtct tcatcttaaa actgcaggaa caacctggct
     7321 tgaggaggtt atcggactag ccgaatcggg tggagaggcc ctggaattcg tcagggaaat
     7381 ctactcggat gccctcacgg atatcgaaga gctttgcgca ccctatgccg atgtaatcga
     7441 tattgatgcc ggggcgcttc cctcttccgg cgaagtggct ggctgggaca gcagccggtt
     7501 tgcaggggca ctgagacatg tcccgggtaa tcctgactat aatccggata tgaggcagct
     7561 gatacatgtc ggttacaaac tggctgcaat gagaatggat gaatatttca ggctgctgga
     7621 ggcaaatgag aaaactgttt cggcctgtgt ctttgaaaac atgtacaaca ggcatatctg
     7681 caggctgttc ggattagaat aatcagagac ttcccgcgcc cggtaccaaa gtgaggatta
     7741 tcaggattca gggataaaaa tcataatatt agcgttattt tctttcatga taaaggttaa
     7801 taacgctaat gttatttctt ctgaatccct gccctgaatt gaggtactac cggaggaatt
     7861 ccggtttctc cggcaatttt catcaaatgt attatatcat tcaccagggg aaaatccact
     7921 cccgatttaa acaacaccct gatttcttcg ggagaatcgg taccgaagaa attcactctg
     7981 ataccctttt ccttaagtct ccgggtataa tcaggcaatg aaggtgttat cggctgtgta
     8041 agctgaatga attcagcttc cgattcaatt gtttcattta tatagttcag attgtcattc
     8101 tgcctgtcca tattacatat cattattccc tgaacagctt cccttgccat ttcagctgcc
     8161 tttctgctgc atgcaaggaa ggcctggtgg agcctgtttt ccgaagctat tttttccgcc
     8221 accatcacag gcagtctccc ctcacctttc aggtgtatgt taagccagac attaaagggc
     8281 attacagcca ggacctcatc gagagtgggt atttcagccc cggcaaattc cgctgatttc
     8341 cagctgcctg catcaaggct tttaatttct tccagggtca ggtccgatac ttttccgctg
     8401 ccattggtgg tccggtcaac tgtttcatca tggataacca ccatatgatt atcccttgta
     8461 aggaaaacgt caaattctat catatgggca ccggcaataa ctgcctcacg gaatgccggg
     8521 attgtatttt caggatgtgt ggccatggct ccgcggtgtg cacacaatcc tctttccgga
     8581 agaatgattc cggatccgta taatccccct tcagaggcaa gaatcagggc cggcagggca
     8641 aacagtgctg caataagaga tatcttccgc ttcataatac atgattctta tgttcagaaa
     8701 aaaatctgcc ggaaataatt ttccggcaat tccgacagga ccaagttaat attaaagaat
     8761 taataatctc ccgggacagc agataaaata ttcgttccta ttcaagagtt ctgacagcaa
     8821 tcaccggttt gggatccgtc atgtgcaggt caagaattat tatttcatta agtccctgtc
     8881 tgataaagga tgcaggacag tagagccttt tctgcggacc tatatcccag tagcgtccaa
     8941 ggttatgtcc gtttacccaa acaatgcctt ttttgtagtt tgacacatcg aaataggtat
     9001 ctccggttat tgccagtgag aaatttccct tgaagaagac tccaggtttg ttaatgacct
     9061 ttccggatga acggaggtca tatatatatt tccagtccat cggcagcttg aatatctccc
     9121 agttcatgag cgtcattccg ttcagggtca ctctttccgt aattcctttt ctatctataa
     9181 gatgctgtgc aaaattgata cgccccattg cttcgaccag tatttctagt accggcacat
     9241 cagccttgct ttcgggtaat tctatcgtgt taatcccctc ccttctgtcg agatgtccta
     9301 tatattctcc gttcaggaat acagtggcat aatcatgtat atctgttatg gtaagccagc
     9361 cttttttatg tcctatcagt ctggtgcggt agagaataaa accgtaatcc tggccatagg
     9421 cctcaaaggt attcggcgta acggaactta caggttcagg aagattatcc caaattgatg
     9481 taaacttggt cgggaaaata aggggaattg taatggcggg aatggtatca ggtattttag
     9541 gcagtttttc atttttcgga agatatgatc cgataagatt cctcagtgca aaatattttg
     9601 ccgttggcct tccctgttca ttaatcgggg catcataatc atagcttgtc acatccggtt
     9661 catatccctt tctgcctgag tttgcacctg cggtaaatcc gaagttggta ccgccgtgta
     9721 taacatagaa attgaaggat ttcctgttat ccatcagaaa tttaacttcc tcaagaagcc
     9781 ttaccgtatc gggtctgttc cattcttctc cccagtgagt cagccagccc ggataagttt
     9841 cactgctgaa taccggtaca ccgggattca tttttgcggc cagctcaaaa tcggcagccg
     9901 tactgcccgg atccagtccc acagcacttc ccggcagtgt gcctgcttcc agcatgtaag
     9961 tggtggggcc gtcgccggta aaggtcggga catcaattcc attggcctgc catacttcct
    10021 tcagtctctg agtataattc ctgtcgttgg catagctgcc gtattcattc tctatctgca
    10081 gcatgatgac cggaccgccc cttgttatca gataaggccg gatctcatct gcaagacgtg
    10141 acatatatct ttcagctgca gccatatacc gcggatccat acaccggagc ctgatatccg
    10201 gtatcctgag gagatacggc ggtatgccgc caagctccca ctccgcacat acatagggcc
    10261 cgggcctgac aataagccac attttttctt tctgaacaag ctttatgaat tctgccagat
    10321 tacggttgcc ggtagagaaa tcaaataccc cttcttcaga ttcatggtaa ttccagaaga
    10381 tataaactga tatggtattg catcccattg ccctggccat cctgatccgg tgctgccagt
    10441 attcagcggg tattcgtgag ggatggagtt caccgcttat tatctgaaag ggttcatcat
    10501 ccagaagaaa ttctgtcggg ctgagcctga atgtatgttt tgccttctgt gccggaacag
    10561 ttgttcctgc gagaattaag gcaaacagca aaaaataaat ttttagtctg atcatggttt
    10621 gtaattttga gactgtaata tatatcaaaa tcctcaatct tcggctgctt attcaaaata
    10681 tgaccgcctc tgtattcctg acctgctgtt tgttgcggca acatgcacca ctggtctgtt
    10741 attcctatat tgcctggtag tttctgagct ttgcatagaa accatctttc ctgttcatca
    10801 gttcggcatg ggtccctctt tccactatca ttccgtcatc aataaccact ataagatccg
    10861 cgtgctgaat agttgagagc ctgtgtgcaa tgacgatgga tgtcctgttc tccataagcc
    10921 tcagaatggc atcctgcacc agcctttccg actcggtgtc aagtgccgag gttgcctcat
    10981 caagaatcag tatcggaggg ttattcaata tggctctcgc aatacttatc ctctgtttct
    11041 gtcctccgct gagcttattg cccgattcac ccacattatt ttcataaccg gactccgttt
    11101 ccattataaa atcatgtgca ttagcaatcc ttgcagcatt ttccacatct tcctgcaatg
    11161 aggaattcat accgaaggct atattttcat agaaagaatt attgaagagg atgggatgct
    11221 ggcttactat tcccatgagg ttgcggagat cggtaatatt catgtcccgt acatccactc
    11281 cgtcaataag gacagaacct ttatccgcat caatgaaacg cggcagcaaa tctgccagtg
    11341 tggattttcc ggcgcctgat ttgccgacaa tggcaaccgt ctgacctttt ctgatcgtaa
    11401 gattgatatc cttcaaaaca ggttcagtat tgtaagaata ccatacaccc ctgaattcaa
    11461 tctcctcccg gaattccttt acaggaaccg gatcaatcct ttccctgatc ttttcctctg
    11521 cttcgagaat ctgatcaacc ctttctattg atgccatacc cttctgaata ctgaaccagg
    11581 cggttgtgat attttttgcc ggtgggatta tctgggaaaa cacaatcaga taagcgatca
    11641 gtttatcggg agtcatgttt gtctcaccgc taagggccat cattccgcca aggtataata
    11701 gggccattac aacaattgtg gaaaggaatt cgctaagggg tgatgccaga taatatttcc
    11761 ttattacacg ccggaatatt ttcgagtatc tttcattgat ccttctgaac tgttcctgca
    11821 ttttcttttc agcattgaag ccctttacta ttctcagtcc tgtaagtgtc tcttccacaa
    11881 ccgacaggag tcttccaagc tgctgctgct gaacaagcga ggaggaacgc agggtacggc
    11941 ttaccctccc gataaaccag ccacttaccg gcagcagcac aagtgcatat aacgaaagct
    12001 tccagctgct tacaaaaaga tagatcacaa aaatgagaat gtacatcgga tcgcggaaga
    12061 gcatggtaag ggaagacatt accgatacct caatttcctg gacatcattt gatatccggg
    12121 tcataagatc tcccttgcgg gcatcggaaa aatatgacag gggcagcttc aggaccttgt
    12181 caaacatttt tttcctgaga tcccttaccg tgtaggatct gatataagcc atactgttat
    12241 ttgccaggaa aataaatccg tttttcaaca gactggcaat cacaaaaaca acgctgacta
    12301 tgagaagtgc tcccgcctga ccgtgtctgt cgataaagag tgaaaggaaa tacctggaag
    12361 ccgaattgaa atagtcaatg ctgagctgaa aatcacccgg atgctgaagc acatcaaccc
    12421 tgttgaacat gattttcagg aagggaatgg caagggtgta ggaaacaagg gcaaagacag
    12481 cactcagaat attaaaaacc acgctgttaa atgcctgcca tttataaggt gctatatatt
    12541 tgagaagaag ttttatctta ttcatcagct atgtgtaatc ttattaataa cctgtgaaat
    12601 tgaatggagt aagtgccttc agctcctctt ttaccgcttc gcttatttcc agggcattta
    12661 taaaatccat aactgcttcc cgggatatct taacattgcc acgtgtgagt tccagaagaa
    12721 tttcataagg ctgcggatag ccttcccgcc tgagtattgt ctgaatggct tcggaaataa
    12781 ctatccagtt attttcaagg tcatttttta tctcctcatg gtttacaaca agcttgttca
    12841 aacctttcag taatgagctg aacgcaacca gagtatgccc aaggggcact ccaaggttcc
    12901 gcagtactgt tgaatcggtc aggtcacgct gaagtcttga aagcgggagt ttctctgcca
    12961 gatggccaaa tattgcattc gcataaccaa ggtttccttc cgcgttttca aaatcaatcg
    13021 gattgatttt atggggcatt gccgatgaac ccacttctcc cttccttgtt ttctggctga
    13081 agtaattcat ggagacatag gtccagatat ctctcgaaag atcaatgagg acggtgttga
    13141 ttctccgcat attgtcgaaa agtgcagcca gattgtcata atgttcaacc tgtgttgttg
    13201 gaaaagatcg ttgcaatccc agttccccgt tcacaaaatt atccgcaaac agtatccagt
    13261 cgatatcggg gtaagcggcc ctgtgtgcat tgagattgcc tgaggctccc ccgaattttg
    13321 cagaataggg tatctggcca aaatattttg actgttcatc aatacgtgta acaaacactg
    13381 agatttcctt gccgagcctg gtcggtgagg caggctggcc gtgtgtcctg gccagcatgg
    13441 gaatattctt ccattcgtcc gccaggcctg caagcatatc ccttacatca tacagaagag
    13501 gaaaatagac attaatcatc gcatccttca gggacaatgg tatggccgta ttgttaacat
    13561 cctgtgatgt aagtccgaaa tgaatgaatt cactccattt cagccagcct gaatcccgga
    13621 atctttcctt gaggaaatat tcaaccgact tgacgtcatg atttgtcacc ctttcatttt
    13681 ccttgatcct ttcagcatcg gccatactga aatcttcata aaatgaacgc aggacattgt
    13741 aattatcctt ccggaaatca gccagcggag gcaggggaat atcacacagg gaaataaaat
    13801 atttaatttc caccatcagg cggtacctca tcagtccgaa ttctgaaaaa tacagatcga
    13861 gttcattcac tttctgtctg tatctgccgt caatgggaga aatagcagta agagaattaa
    13921 gatccatgat tcagttgtta aatgattgct taaatcataa aaattgtaca caaaaatagt
    13981 atttttgagc ttcttaaaaa aaatgaaagt ggccaggcca aaaaatcaaa gttcagcatc
    14041 cggtaaaatg taatgactcc agaaaaaatc aggatttccg ctgtaaggta tgcaaatacc
    14101 tatccgttta tttatggatt aactgaaacg ggctttgaca ggaaagtaat cctgtcaacc
    14161 gatcatccgg cagattgtgc tgccagactt gctgcgggga aagcagatat cggactggta
    14221 cctgtagcca gtcttccgtt aatcagtaat tatcagatta ttaccgatta ctgcctggga
    14281 gcctacggaa aggtacggac tgtaatgcta ttgagtaact gtaccttcgg ggaaatccgc
    14341 aacatatttc tcgactaccg ctcaagatca tccgtgaacc ttgcaaggat tcttgcaaaa
    14401 cactggtgga aaagggaatt tggctggaaa aataccagtg aaaactttga ttttaaaaat
    14461 attcctgaaa aggacggaat cgtgctcata ggtgatcagt gtttcgagct cgggaaacaa
    14521 ttcagatacg ggactgacct ggcagaagaa tggtatgatt ttaccggcct gccattcgcc
    14581 tttgcatgct ggacggcaaa caggcagttg ccgcaggcct ttctgaaaga attcaatgag
    14641 gctctggcaa caggtgttga taacattccg gaagtggtca gaaaatatgg caggaaagga
    14701 atcataagag gaaaagctct cgaaacatac cttacggaaa atataagttt taatcttaat
    14761 gaggacaagc ggaaagcaat aaaattgttt ctgaattatc tgaaaaaatc tgattagatg
    14821 ctgatattga acatagtgca taacccatat tacttttttc agttttaatc tcaaagatat
    14881 ttttcctata tttgaagata tcacagcatt aattttataa ccgtataaac ttttatcaat
    14941 atggccgtta caattacatt taatgaactc cggaagatca aagacctttt accccccgga
    15001 agtataagga aaattgctga caagcttgac atgaacgagg aaactgtcag gaattacttc
    15061 gggggaagga attttgaaaa gggagaaagt gttggtgttc actttgaaca gggcccggga
    15121 ggcgggattg tatcttttga cgactccacc atccttgaaa tggcaaaaaa gatgattacg
    15181 gaatgagtta acagaatcag cagcatgttc agaagcaaaa gacatgttct tttgcttttt
    15241 tgttactgtc cggattttat ccggaaagcc gttgaattga ttaatgtacc tgcatttccc
    15301 aatgaacttg tcagctataa tatcacagga agaagaaaag agtcttacag cacttgaggg
    15361 cttttttgtt ttgaagtggg gcggtacgaa tcttccttcc cacgatcttc agcatcaccg
    15421 gagagtatgg accaatataa aggaaatcct gaatcattgc aatgatcccg atattgaccg
    15481 ggcacggata agcaatcttt taatggcagc ttatctgcac gatatcggaa tggcacggga
    15541 acagggagtc cgccacggat ttcacggaaa gaaggcagct gaagaatata tatcggcgaa
    15601 ccggctgaag cggaaggatt atgaagaagc tctcaacgca atagagtttc acgacaacaa
    15661 gaattatgac aatgattcag gcaataatcc ggtactgaaa atattatccg tggcggatga
    15721 tcttgatgca ttcggcacaa ccggcatatg gcggtatatg gaaatataca gggcccgggg
    15781 cattccggaa gaaattatcg gatccaggat tcttgaaaat gctgcctcgc gttttgagaa
    15841 ttttaaagca ttttcttcag acaagcccct gctgtttcag aagcacaggg agcgttacaa
    15901 tgtactcaaa cggcatttcg ggaattataa ccagagtatg gctgaagccg gggataaatg
    15961 aggcagaagt ctgaattgcc aacatttgtt caaatgcaga tcagcagtga ttattgcggg
    16021 caaaaaaaat cagcttcagc agcaatcttc ttcattaaat aacataagct gttgatcctc
    16081 acagtcttct gactcgagat tagagatggt gactccaagc aatctgattc tgacattgtc
    16141 gaggctgagt gattttctta tttcggtaac cgcccggtgc agtgcaggga aatcacgtat
    16201 gaaatgagga aaggttttcg acctggtgat ctgtctgaaa tcagagaatt taattttcag
    16261 ggtgactgtc cttcctgttg ttccggcttt ttccacacgc tgcataagtt ccatttcaac
    16321 cttgtagagt tccgttatta tttcaaactg tgttgtcaga tccttctcat aagtaatctc
    16381 ggtaccaatg gattttctct cggattcagc cagtaccggg cggtcgtcaa tccctcttgc
    16441 gatatcatag tagaatttcc cggcctttcc gaaattcctg accaggaagg caaggtccgg
    16501 tttcttcagg tcagctccgt tgtgtattcc cagtttatgc atcttttcgg cagtaacctt
    16561 tcctatgccg taaaattttt cgacagggag tttctcaatg aatttttctg cttcctgcgg
    16621 gctgatcaga aaaagcccat ccggtttttt tatatcagaa gccattttgg caagaaattt
    16681 attgaaagaa actcctgccg aagcagtgag tcctgtcttt tgcctaattt ccttctttat
    16741 ttctcctgca ataattgtgg cggaacctat atttttcctg tcccttgtaa cgtcaagaaa
    16801 tgcttcgtct attgaaaggg gttcaaccag atcagtatag tcgctgaata tactgaaaac
    16861 ctgtcccgaa acttcattat accggtcaaa cctgtgcctg acaaaaatca gttccggaca
    16921 cagccttctg gcagtaatgg agggcattgc agaccgtaca ccgaatttcc tggcttcata
    16981 gctagctgca gcaacaacat gcctctcccc gcttccgccc actgcaacag gctttccccg
    17041 cagctcagga ttatcaagct gttctacgga tgcaaaaaaa gcatccatgt caatatgtat
    17101 gatcttcctc aattcatccc aaaactaaga agaaattagt ttcatttccg ttttgtaaat
    17161 atctttggct ctctttaaat ttaacgattt attttttgct cttgcaatat ccagccctca
    17221 acatcaggca aagatttgcc cttatcctta catcacacag gctctgggga aaattgttcc
    17281 tgccatttat tgtgtcagaa gttcctggaa agaaatattt cacattgtca gaatcccttc
    17341 ttccatatcc ttcggatgac actttgaaaa tactcgaaaa tgaagaaata gaacttgtca
    17401 gaatcaccaa tgattactcc gataaacaaa tattcaggat ttattcaaga gaaaagaatg
    17461 taaaggattt tctggaaaat gtaaaacctg aaataacagg aaaatacata aggccgtata
    17521 tagagagaag aatcagcagg tgcttcttca taataagaga cgagggaacc ctctgctttc
    17581 agagaagaga acaatctaac attctttatc cggaggatgt tctcgaaata attcctgatt
    17641 ctgcaattcc ggtattcaga tttgacagag gaaaggaatt cactacctat cagctcaccg
    17701 ttgaatcaga gggagagaaa attgaattat gccgcaaagc ggttgatatt ttatgcaata
    17761 atccttgcat tatcaggcat ggaaataaaa tctttcagat aagcggtata gacgggcaga
    17821 aattacgccc gttccttgaa aaggaatcgg taatcattcc gaaaaattca gaactgaagt
    17881 attattcagg atttgtactg aatgtcgtaa ataattgcac tgccgaaggt aaaggattta
    17941 aaatcattta cccggaatct gaaaaatggc cggggttaag cctggaaaac agcatcatgg
    18001 gatacccggt actcattctg agttacaatt attcaggcat aaccatttca cccggtgata
    18061 ataatgaata tttcacagtg ttcgcgcatg aaaacggtga attcatttac cggaaatata
    18121 aaagggattt ccgttgggaa ttgcaatgcc gggaaattct atccgagatc ggcttcaatt
    18181 ctgaggataa gattaatttt tctcttcagg atgaaaatct gaaacctgta tctcagataa
    18241 acaaggttat tgaagctgtc aacagaaatt ataatcacct tgtctcacaa ggtttcctga
    18301 tatcttcgaa gcatctggat aaaaattaca acctcagaaa catcagcatt gaaatcttca
    18361 acaagtctga aggtgactgg tttgacctgg atgctgtaat aaagatcgga gaatgggatt
    18421 tttcgtttat aaggttcaga aatcatatat tgaacggcaa cagggaattt gagcttcctg
    18481 acggaaccac agccattctc cctgaagaat ggctggtcag atacaaaaac atttttgaat
    18541 ttggcaagga tcatggagat tccatcagaa ttcacaaaca acactttccg ctgctcacgg
    18601 aggtattgca aaacagagag gatgaatcat tcaggaatct ggaaaaactg ctcctgccgg
    18661 aaaaactgcc tgttcctgaa gtacctgcgg gattaaactg ccggctgagg gattatcagc
    18721 ttgaaggact ggcatggctt cagtggctgc agtcttcccg tctgggaggt tgtctggccg
    18781 acgatatggg actcggaaaa acgatacagg ccctggccat gctgcagatg aacaaggaaa
    18841 cctctgataa accgggaaaa tcagctacgg ttcatgaacc aacacttttc gacgacccgc
    18901 cagtaaatct tacctcgctt attgtagtgc ctcccacaat aatgcataac tgggaaaatg
    18961 aaataaggaa atttgtccct cagatgaata tttatataca cagaggttac cggaggctcc
    19021 gcagctgttc cgatttcatt aagcacgaca tcattctttc aagctatcat actgtaaaac
    19081 aagatataga cttattgtct tcattccatt tccattatat tatccttgat gagagccaat
    19141 acatcaaaaa tccggcatct cttgcataca agtgtatgat caggcttaaa tcggattaca
    19201 ggctcgttct gacgggaacc ccgatagaga attctgtaac cgacctgtgg gctcagctga
    19261 attttgtgaa ccggggcatg cttggaagtc tgacctggtt caggaataca tatgcacgcc
    19321 ccattgaaaa agacagggat gatctcacgg aaaacaggct aaaaaagcat ataaaacctt
    19381 tcatattacg ccgtacaaag gaaatggtgg ctgccgatct gcctcctgta tctcagcagc
    19441 tgattttttg cgaaatgacc gtagaacagc aaaagatcta tgaatcagaa aaatcagcag
    19501 taagaaacgt tattctggag ggtcaggccg ccgggaagaa gggaaagact gccatcataa
    19561 ttcttcaggg actcatgaaa ctccgccaga tatcgaatca tcctgttttg actgacgaag
    19621 attattcata cagttcaggc aaattcgagg ctgtgatgaa tgatattgag aatgttgtaa
    19681 acgaaggaca taaaattctg gtcttttctt cttttgttat gcatcttaaa ctatttgaag
    19741 aagctcttgg caaaaaacag ataaagtata caattatgac cggtgcgagc aaagaccggg
    19801 aaaagttaat aggcagtttc caaaacgatc cttcatccag ggtttttctg atttctctca
    19861 aaaccggagg tgttggacta aacctcacat ccgcggatta tgtattcata cttgatccct
    19921 ggtggaatcc ggctgccgaa cagcaggctg tcagcagggc tcacaggata ggccaggata
    19981 aaaatgtctt tatttacaga tatatttcct ccggtaccct tgaggaaaaa ataaccaagc
    20041 ttcaggaaag aaaatcaaaa ctcgccggaa catttatagc aacgacaaat cccctgaggg
    20101 acatggatat gcatgaaatc ctggacataa tcgaatgaat cacaattgaa gcccgattag
    20161 taagcctcgc gttcagtgta atgtgtatga agcaattcat gagcccgttc actgttgggc
    20221 tgaccaagga attcctcgta tatggcaatc acatcagggt tatcatgtga tttacggaat
    20281 tccatatgtt catcctccga ataaatagca ttcatacgtc tttttcttac ttccgcattt
    20341 gtaggtatgg gctggcctcc gccgccaagg catccgcccg ggcatgccat tatttcaatg
    20401 aaatgatatg aggcctcgcc gctcctgacg agtctcatca gcttgttcgc attagtcagt
    20461 ccgtgtgcta ttgcaacctt aagttcggct ccctcaagga atttccagtc atccacgcaa
    20521 tcctcgattt ttatcgatgc ttcctttata ccttccattc cccggacagg agtaatattg
    20581 agatttttga acggaacttc tcttcctgtt acaatttcat aagctgttct cagcgcagct
    20641 tccataactc cccccgttgc tccgaatatt acggcggcac cggtcgattc acccattact
    20701 gaatcaaagc ctgagggttc aagatttctg aaatcaatac ccgcctgttt taccattatg
    20761 accagttccc gcgttgtaag cacataatcg atatccttgt aaccgcttga cctcatttcc
    20821 ggtctgtcag cctcatattt ctttgcagta cacggcatga ttgaaactga cactatggta
    20881 gaaggatcca ggttcttctt ctgtgcataa taggtttttg caagtgcccc gaacatctgc
    20941 tggggtgatt tgcaggtaga aaggttattc agaagctccg gatacttgtg ctcaataaat
    21001 ttgatccaac ccggtgagca ggatgtgaac attggcaggg cggtgttttt atccccatca
    21061 acaagaactt tcttaagtct ggtaagaaat tctgtacctt cctccattat tgtaaggtcg
    21121 gcactgaaat cagtatccag gacagaatca aagccgagct gtctcagggc tgtaaccatt
    21181 ttccccgtca ccctttcacc gggatcatat cccaggtctt cccccagggc aacccttacc
    21241 gcgggagctg tctgcactat tacatgcttg ccgggatcat aaatggcatc ccatacctgg
    21301 tcgatatatg ttttttcaac cagggcacct gtaggacagc ggttaacaca ctggccgcaa
    21361 tttgtgcata caacatgact cattggcttg tcatggaagg atgaaatttt ctggtgcatt
    21421 cccttatttg caacggcaat tgccgagata tgctgtaatt ccgtacaggt ccgcacacat
    21481 ctctggcatc tgatgcattt gctgtcatcc ttgatgaatg aaggggatga tctgtcaatg
    21541 acataaggat gatcctccac cagatcgaga aatattgtat ccccgaatgc gaattcattg
    21601 gcaagggact gaagttcaca attctggttt ctgtaacact ttgtacagtc agacctgtgt
    21661 tcggaaagaa gcagttccac aatatgcttt cttgcatttc tcaccttgag cgtgttggta
    21721 tgtatgtcca ttccttcggc tacaggcatg gcacatgcgg ctataagcgt acgggcaccc
    21781 gtctgttcca ccacacatac ccggcagttt ccggcaacac aaagatccgg atgattacac
    21841 aaagtgggta tgtttatatt caacttccgg gcagcatcga gaatagtggt tccctcctca
    21901 acagatatct tttgattatt tatactcaga tttatcattt ttttcgaaat gtttggttat
    21961 taatagataa tctcttccct gaaattctcc acaattgttt tgaaggcatt tccgactgat
    22021 tgtcccagtc cgcattttga tgcaaccttc atggtatcgg caagttttat cagttcagtc
    22081 agataggttg actttacttc tcccttttta actttctgaa ctccttttaa caattgctgg
    22141 caacccactc tgcagggagt acattgtccg caggattctt cagcaaaaaa gtcaagataa
    22201 ttatgaagaa cattgtacat cgacctggaa ctgttgaaaa gtttcattga tcctcctgtg
    22261 ggaacaccct caaatcctat tattgtatct tcaaagcgtt ttcttggcac acagaagccg
    22321 gaggctccgc caacctgcac tgccttggta tccccatcac cgaattcatt cacaaatgcc
    22381 tggactgtca tacccagttc aagctcaaaa atcccggggg tcggtgtatc tcctgaaatt
    22441 gaaaatactt tcgatcctct ggaatccttt gttcccagct gactgtatga ttccggacca
    22501 tgtttgatca ccatcagtgc agtaactagt gtctcgacat tattgattga tgtcggctta
    22561 ccgaaaactc ccgacaccgt gggatacgga ggtttgttcc tgggctctcc gcgaaaacct
    22621 tcaatcgact caaaaagtgc actttcctct ccgcagatat aggctccgct gcccatgcgg
    22681 taactgatac tgaaatcaaa accgatctct ttgataagcc tgtggaattc atcaagctct
    22741 gccagaagct ggggaagcag gaatttatat tcccccctga gatatacaat accggttttt
    22801 gccccgacag cctttcctgc aataaccatt cctccataaa ccttataggc aaccctgtca
    22861 agtatctcac ggtccttgaa ggttcctggc tcaccttcat ctgcattgca aactatatat
    22921 ttatcgggat ccttctcggc agcagtgtat ttccatttta accctgtggg gaaaccggca
    22981 cctccccgcc ccttaagccc cgaatcaagc actttctgaa taatctcagc ctggggcatg
    23041 ttataggcaa ctgtcagtac ttctctcaga tctactttct caaaaatgag atcaacctta
    23101 tttaatcctc tttttttcat agtttcggga tattattttt tttgcatata actcttgatt
    23161 atctcaacaa ccttttccgg cgtaagctcg gtatagggca tttcattgat aagcatcgcg
    23221 ggagccttgt gacaccagcc tatgcagttg gtttcaagca aactgaactg tttgtcggca
    23281 gttgtctccc ccacttttac cttaagcata tcttcaagag tctcaagtat ttcattcttt
    23341 cccttcatcg cacatgtaat agtcttgcat actctgatca catacttgcc cctttccttt
    23401 gtgtcaagga aagtataaaa tgaagctgta ccataaactt cagcagctga aatatccagc
    23461 tgatttgcaa tttcaaccat cgcctcgtca gtcaaataac tatgtctttc aaccacaccc
    23521 tgcaaaatgg gcataagact ttcccttgac ctgccgtgct tttcggttaa ttccttcaca
    23581 agatttccca attgatacat ttccaatcgt ttttattaaa aatttatatc ttttacagta
    23641 acacttacct gttttaaagg tgctgtgaat ttaataacaa tactcatata aaaacaaaat
    23701 tattgtcata gttaataatt tataacaagt ataaaaaaga attaaggtta ttaaattgct
    23761 gattatacga aaattatccc catttgtgtc attccgggca atgttaaaaa aatcctgcaa
    23821 taatctgtta tttctgtttt ttgttaaaaa atccactata cagaaataag gctgaacata
    23881 ttctggtaat tcagcctgaa aatatttatt atgggaaata tatttcctat attgtgagag
    23941 atattacaga aaaatttaaa agaactaact tacaggaggt ataatatggc tgtagaattt
    24001 ctctgcaagg tgtgccgggg tcacctgaaa ccgaagacat caataattct gaccgctacc
    24061 aaccttaaaa accgtacgga aaagggtctg gtatttctta atcccgaaat cggaaactat
    24121 acacatacta cccattcgtc atttcagatc aggaaaggtg aggaatacat ttttacatgt
    24181 ccgatctgca atgcgcagct gaacagcatg aaatatctgc atctcgtcag aatcctgatg
    24241 atagatgagg gaggcaatga atccagcatt tatttctcgg gaatcggagg agaaaagtgt
    24301 acctacaaga tccgggatga caaagtggaa gcaagtaccg gtcctgatgt aaaaatttac
    24361 aacaaatact ttgatgtccc tgaagaagac aggaaatacc tgtgatctcc ggtaattttt
    24421 atttttctgt taacaattaa ttctattttt gttgccatta aataattaat ggaaaaggct
    24481 atactatgaa cactgatgta aacaaactgg cagccgacaa tatcaggata ctgtcaatgg
    24541 caatggtgga aaaggcaaaa tcaggtcatc cgggaggtgc catgggtggt gctgatttca
    24601 ttcatattct gttttccgaa tttctcaggt tcgaccctga tgattcttcg tggataaacc
    24661 gcgacaggtt ttttcttgat cccggccaca tgtcaccgat gctgtattcg gtactgaccc
    24721 ttacaggtct tttcagtatt gacgacctca aacagttccg ccaatggggc agtccgacac
    24781 cgggacatcc ggaactggat gttgccaggg gtgtggagaa tacttccgga cctctcgggc
    24841 agggacacac aatggctgtg ggagccgcca ttgcggaaag gtttctggct gcaagattcg
    24901 gagaactgtt cgcccataaa acctatgctt tcatctccga cgggggaatt caggaggaga
    24961 tatcccaggg agccggacgg ctggccggcc atctgggcct gaataacctt atcatgtttt
    25021 acgattccaa cgacatacag ctttcaaccg aaaccagtgc cgtatcaacg gaagatacag
    25081 ctgccaaata catggcctgg ggctgggaag ttgttaccat tgacgggaat gatcacgatc
    25141 agataagaaa agcactctca caggcccggg aatcaaaaga caagcctttc ctgatcatcg
    25201 gaaggaccat tatgggtaaa gggctcattg acaatgaagg aataagtttt gaaagaaaaa
    25261 tatccacgca tggaatgccg gtaagtgatg ccggtgcttc ctttgagaaa tcccttgcag
    25321 gcctcggggg caacctgtcg gatcccttca caatatttcc tgctgtaaaa gaatattatg
    25381 cagctgtgat tgaagaaaag cgcagatatg ccaccgactg gaaaaggcag aaaaaagaat
    25441 gggcagcaga aaacagcgat tccgaacgta aactcaacct gttttattct ggcgacatac
    25501 ctgccatcaa ttttgagtcc atctcccaga aagagggaat agcaacgagg gcagcctcgg
    25561 ccgctgttct cggagttctt gcaaatacca ttgaaaacat ggtggtcgca tcagctgatc
    25621 tttcaaactc agataaaacc gatggttttc tgaaaaatac aaaacctctt gttaaaggag
    25681 atttcagcgg tgcatttctt cagaccggag tatcggagct gacaatggca gcagttatgg
    25741 ccggaatggc tctccatgga ggtattatac ctgcctgcgg gacattcttt gtattttccg
    25801 attatatgaa accggcagta aggcttgcct gcctgatggg attaccggta cgcttcatct
    25861 ggacacacga tgcattcaga gtgggggaag acggtccaac ccatcagcct gtagaacagg
    25921 aagcccagat acggcttatg gaacatctta aaaaccataa gggtgagaac agcatgcttg
    25981 tactcagacc tgccgatgca cttgaaacaa atgttgcctg gaaactggct ctcgagaata
    26041 aaaaaacgcc aacggctctc atcctgtcaa ggcagaacat cagggatctc ccctcttcaa
    26101 ctggcagcag atacagggat gctctcatga tgagcagggg agcctataca gtatcggact
    26161 gcaagggcag gccggatatc attcttgtgg ccagcggttc ggaagtggca accctggttg
    26221 agggagcaga acttctgaaa aaggacgggc tcagggtgag gattgtttca gcaccctcgg
    26281 aaggtttgtt cagaaatcag gatcaaagct acagggaatc ggtcattcct gacgatgtgc
    26341 ctgtgttcgg acttaccgca ggcctgcccg tcacattaca gggccttgcc ggtccgcgag
    26401 gaaaagtctg gggcgtaaat tccttcggtt attccgcccc ctacaaggtt cttgatgaaa
    26461 aactggggtt tactgccggg aatgtttacc gccaggtaaa ggattacctg ctacattata
    26521 aaaagtaaac attatgatca ttcgtaaaaa ggccgcctca tcagcggcct ttatcattta
    26581 tgcagattac ttttgtaatc tgctgatttt gtattaaatt cgatcgtgaa tttcaagtca
    26641 gcaacgtcaa ccataatttt gcaatatgaa aaggatcctg ttcatttcag ccctgatgct
    26701 tgccatgggc atcaattcct ccttcagcca gaacgattca atcagaaagg aatatgccgt
    26761 cagggataca atcagcgaag atatcattct gttcgacagt gatgaattgc tggatataga
    26821 actcagattc aacataacac aatacagaag gaaaagggac aagagtgaat atctcgatgc
    26881 aacccttaca taccatgtca gtcaggatga ttcaatcatc aaatatctta aaatcaggcc
    26941 gcgcggtatt tcgcgtctta caatatgtga tttccctccc ctcctgctta actttaaaaa
    27001 gaatgacgct gtgccgggag aattcaacag ggttgataaa ataaaaatgg taactcactg
    27061 cagtgccggc ggcgaagata atctgctcag ggaattcctg gtatacaaaa tgtataatgt
    27121 gctcactgat tacagtttca gagtaagatt gctgagagtc aattatgtta acagtgaaaa
    27181 gcctgacaaa aaacaggtaa gggaatttgc cttcctcatt gaacccgtca agtctctggg
    27241 caagcggaca aattcagtgg aagttgaaac agctcatgtt acccagctgc atattaaaag
    27301 agaaatcatg gacagaatgg ccattttcaa ttacatgata ggaaacaccg actggtcggt
    27361 tccaaccagg cacaatacgc ttaccatgac ggaaggcagc tacggcggcc tgggatcagc
    27421 tataatagtc ccctatgatt tcgactatgc cggtgtggta aatacacatt atgctgttcc
    27481 ttttgaggga ctgggcctgg aatccgtccg tcagcgcaga tatcttggtg tctgcaggac
    27541 agaacaggaa ttcagcgagg cactggttga attcatggaa aagaaggaag aaatatacag
    27601 agtgatcaat gattttccat atcttagcgg aaaatccaaa aaagatatga ttaactatct
    27661 tgaaggattt tacaggagcc ttgacaagcg caacagcatt ataaactccc tgagaagaga
    27721 ctgtattata atttgaatta tcagatcctg ccgtccatgt catttgaatc catcgcaacc
    27781 gaaacgaccc ttttgaatat catgatcagg tttgccgtta acctgctggt cctttttatt
    27841 ctcataagat ttatctatta ccgctttacc aggaaggaag agtatgtatt ctcgcttttc
    27901 atgatgggta taataatttt tcttctgatt tcattgcttg gaacagtcaa catacaactg
    27961 ggaatggctc tcggcctttt tgccattttt gcaatactcc ggttcagaac agtcaattac
    28021 acggcaaaag acatgaccta tgtatttacg gtaatcggag tctccatcat caattcacag
    28081 gcaaatattc cgcctccggt cattggtgcg cttatcgtaa atacaacaat tcttgtatca
    28141 gcagtaatac tggaattatt cctcaaaaaa cgggcattgt cgacgatgat tgtaaacttc
    28201 agcaagccgg aacttctcag gccggaacgc aggaaacaat tacttgagga attatcagcc
    28261 cagacaggtc aggaaattgt aaaaatctcc gtcagaaaaa ttgatgtaaa caagggcagc
    28321 attgaacttg aagcttttta tgcagacaag atagctgact gaaggacaat aacttccggc
    28381 agcacacatt ctgaagctgt atttgtcctt ttttgattaa gtttgcacct tgtttttatt
    28441 tatcatgcaa tacgatattc aacaaggaat aaaaggtctt gtttttgatc ttgacggcac
    28501 acttgccgac actatgccct accatttcca gggctggaaa aaggcctgtg aaaaatatgg
    28561 cgccaggatt gataatcatt ttctcagaaa atatacagga agccccagct ggatagttgc
    28621 cggagaggta ctgaagcatt gcggccttaa caacagtgtg accattgaag aaataatgaa
    28681 tgaaaaacac agggaattct tcaggcttca gcatcttgta aaaccgatca caccggtaac
    28741 tgaaatcgta aaaaaatatt acgggaaact gcccatggcc gttggaacag gcgggcacag
    28801 ggaagcagtt gaacgcacac tggaaataac cggcctgaga gaatattttg acatagtagt
    28861 aactgccaat gatgtgaatg cttttaagcc cgatcctgaa acatttctga aatgtgctga
    28921 actaatgaat gtcgaaccac gtgacattga ggtgtttgag gatgcggagc ttgggatccg
    28981 tgcagcaatc agtgcaggca tgaaagtgac tgacgtacgg tcctggtaca attctgactg
    29041 gtaattccac acctgtatcc ggcctgacat ggctatcctt taatctgccg gagttcagct
    29101 cttctttctg attaatgcct gaatgccgga aaagccggtt gtgatgaagg aatcagaata
    29161 ttaatcccta ttgtgttaaa acatcttttt catacggaaa attcaaatcc tttttctatt
    29221 ttagaaaaat aaacatattc ccctttttct gagttgtaaa aaccatcatt gctggttgct
    29281 ttgaatactg tttatgaaat cataatcagt agagggacta tgataattac taattctaaa
    29341 aaactattct tatgaaactc gatcgcaggc atttcttaaa actgagcagt attgccggaa
    29401 caggtattgt aggttccggt attttgcagt catgcaaacc cggcagcggg ggaaaagcag
    29461 tcagtgcact ggatttgata aaagagcagt cacttgcaaa acatccacag aaattcaata
    29521 tgtgcggata tgccgcgccc gcacttgatg tggttagaac agcattcata ggactgagaa
    29581 gaggcagcac tcatctgacc agtgtcagcc ttctggaagg tgttgaaata aaagcattgt
    29641 gcgaccttgt tccgggagtg gttgacaatg caatgagaag aattgaacac acaggattca
    29701 aaccggatac atataccggc aatccggaag cctggaaaaa aatgtgtgaa cgggatgata
    29761 tagatcttgt ttatattgcc actccatggg atatgcacac cccgatggcc gtttacgcaa
    29821 tggagcatgg caaacatgta tgcattgaag ttccggcagc agttaccatg gacgaatgct
    29881 ggcaactggt ggaagtatcg gaatcaacgc agaagcattg tatgatgctc gaaaactgct
    29941 gttatgattt ttttgaaata ctgacgctta acatggcaag acagggattc tttggtgaaa
    30001 ttgttcatgg tgaaggtgcc tatatacaca ctctgctgga ttccaatttc tccaaaacag
    30061 gttactggga tatgtggcgc ctgagggaaa acagcagccg tcaggggaat ctctatccaa
    30121 ctcatggtct gggtcctata tgccagataa tggacattaa caggggtgat aagatggaat
    30181 atcttacagc tgccatgagc aatgatttcc aaatgaatga aactgccaga aaacttgccg
    30241 ccgtagatga tttttacagt gaatttgtgg accggcccta ccgtggcaat atgacaacct
    30301 gtacaataaa aacaaacaag ggcaggacaa tcatgctaca gcatgatgtc acttcaccaa
    30361 acgtatattc ccggatacat aaggtcagcg gaactatagc agcagcactg aaatatccct
    30421 tgcccccaaa aatttccgtc ggccatcagc gctggctcaa tgaagaagaa tttaaggaag
    30481 tggaagaaaa atacactcct gaaataatca aaaggatagg agaaatggcc aaacaggtag
    30541 gaggccacgg aggaatggat tttctgatga actggaggct gatcgactgt ctgcgcaacg
    30601 gactgccctt cgaccaggat gtatatgatg ctgcactgtg gagtgccgtg ggacctttaa
    30661 gcgagtggtc cgcagccaac agatccaact ccattgatgt tcccgatttt accaacggcg
    30721 catggaagac caacagaccc gtggacctgc ttctggaaca aggcggtaat accagggtga
    30781 taaggtctaa tgctcccccg agacgcaggc cgcagccggt agctgaagga gttgaataat
    30841 gctttcagga accatagaaa ttcttaggct actatgaata aaatagaaac aagtcaacaa
    30901 gcaacccttt cgcaaagggt tgcttctgtt gatttcttcc gcggatttac catgttcctg
    30961 ctaattggtg aaggaacgcg tatttacaat agctta
//
LOCUS       DAOSPX010000005        29564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_1726_length_29564_cov_4.669084, whole genome shotgun sequence.
ACCESSION   DAOSPX010000005 DAOSPX010000000
VERSION     DAOSPX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29564
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1726_length_29564_cov_4.669084"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLZ75_00555"
     CDS             complement(<1..221)
                     /locus_tag="PLZ75_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOS70918.1"
                     /translation="MGPDERKRLSASIKGRAAALGFDLCGIARAGILADDGRILEQWC
                     EAGMNAEMSFLRRDIPRRINPEFLLAGAKT"
     gene            complement(230..550)
                     /locus_tag="PLZ75_00560"
     CDS             complement(230..550)
                     /locus_tag="PLZ75_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOS70919.1"
                     /translation="MVSSQQIDVESRIIDSLRTVYDPEIPANVYDLGLIYEIRVDDGA
                     VAHIRMTLTNPNCPVADHLLEEVREKVGKVEGIEKVDLKLTFMPPWDPDMLSDEVKLE
                     LGLL"
     gene            complement(603..1034)
                     /locus_tag="PLZ75_00565"
     CDS             complement(603..1034)
                     /locus_tag="PLZ75_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOS70920.1"
                     /translation="MTVNEVQDAIIEDFSVFDDWMDKYNLLIEMGRDLPVIDPGCKTT
                     EYLIEGCQSKVWLCPSFENGKIFFTADSDAIITRGIVALLIRVLSGRTPAEIISADLY
                     FIDRIGLHQNLSPTRSNGLLSMVKQIRLYAIAFNAKQPPAV"
     gene            complement(1039..2256)
                     /locus_tag="PLZ75_00570"
     CDS             complement(1039..2256)
                     /locus_tag="PLZ75_00570"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOS70921.1"
                     /translation="MKDISAIRADFPILSRNIYDKPLVYLDNGATTQKPKCVIDAATE
                     VFTLYNSNIHRGVHFLSDRASEEYENAREAVRSFINAGSSEEVIFTSGATGSINAVAF
                     SFGERFVGRGDEILISHLEHHANIVPWQMMCRRKQAVLKVIPVDDNGELIFEEYLKLL
                     SKKTRLVAVTQASNALGVILPVDRIIGAAHAMNIPVLIDGAQGVQHGVTDVREMDCDF
                     YVFSGHKIYGPAGIGVLYGRAGLLSELPPYQGGGDMVKNVTFEKTSYNDLPFKFEAGT
                     VNFPGAIGLARALGYLLSLGLDNISQRERELLEYATGQMHSIKGLRIYGNSPHKISII
                     SFLLKDIHMYDTGMILDKLGIAVRTGTHCAQPVMDRFGIEGTVRASMCFYNTHEEIDR
                     LCAGIEKVISMFS"
     gene            complement(2269..3627)
                     /gene="sufD"
                     /locus_tag="PLZ75_00575"
     CDS             complement(2269..3627)
                     /gene="sufD"
                     /locus_tag="PLZ75_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOS70922.1"
                     /translation="MNNSTVKYDKYSALYRDNIERISSGSSQYINSFRESAIREFVRL
                     GIPTRKNESYRYTNLDPFFSHDYSNYFIPGEQDFIRAEEFRCDVTDMDVHAIVLLNGF
                     YPTITDKIRKLPGGVLIGSLNEASKLFSDQIEKHYGKYVNGNSDGLVYLNTALASDGV
                     FIYVPEGVEPDKPIQIINLVESDDDIFNQHRNLVIVEKNASVTLIICDHTLSPRKFLT
                     NAVTEVYVGENSHFDIIRVQNEHNNAAKITHTFIHQERNSHASSNNITLHGGLVRNST
                     HHYLGGERAECNSYGLFLADKFQHVDNFVNVIHAQPYCTSNQLFKGILDDMATGAFNG
                     RIFVDKNAQGTLAYQKNNNILLNDDARMDTKPQLEIYADDVKCSHGATVGQPDNDALF
                     YLQSRGIGRHEARLMLMFGFAHEVIENIRVEALRDRIDSLVMQRLKGELSRCAYCMIN
                     CG"
     gene            complement(3620..4387)
                     /gene="sufC"
                     /locus_tag="PLZ75_00580"
     CDS             complement(3620..4387)
                     /gene="sufC"
                     /locus_tag="PLZ75_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOS70923.1"
                     /translation="MLTINNLKASINGKTILKGINLELKAGEVHALMGPNGSGKSTLA
                     AILAGREFANILEGTVTYMGKNLLEMPPEERSREGIFLGFQYPTEIPGVSMVNFMKTA
                     VNEHRKHRGLEPLSASDFLKLMREKKELVEIDSKLANRSVNEGYSGGEKKKNEIFQMA
                     MLEPKLAILDETDSGLDIDALRIVANGINKLRHPERSVIVITHYQRLLDYIIPDIVHV
                     LYDGRIVKTGGRDLALELEEKGYDWIKKEADKSTVYE"
     gene            complement(4412..5860)
                     /gene="sufB"
                     /locus_tag="PLZ75_00585"
     CDS             complement(4412..5860)
                     /gene="sufB"
                     /locus_tag="PLZ75_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOS70924.1"
                     /translation="MEKNQDQILNDVTSGEYKYGFYSDIEIEKIPKGLNENTIRIISE
                     KKGEPGWLLEFRLKAFRRWQTMKMPEWANLTIPEIDYQDIVYYAAPKQRKTSGSNDEI
                     DPELKRTFDRLGIPLEEQKHLAGIAVDAVMDSVSVKTTFRETLAEAGIIFCSFSDAVK
                     EHPDLVRKYMGTVVPYADNFFAAHNSAVFSDGSFCYIPKGVRCPMELSTYFRINAANT
                     GQFERTLIIADEESYVSYLEGCTAPMRDENQLHAAIVEIIAEKNAEVKYSTVQNWYPG
                     DREGKGGIFNFVTKRGLCRGENSKISWTQVETGSAITWKYPSCILRGDNSTGEFYSVA
                     VTNNHQQADTGTKMIHIGKNTRSTIVSKGISAGFSQNSYRGLVKVHRKASGARNFSQC
                     DSLLLGDKCGAHTFPYLEVDNLSAIVEHEATTSKIGEDQIFYCNQRGISTEDAIGLIV
                     NGYAKEIINKLPMEFAVEAQKLLQISLEGSVG"
     gene            complement(6117..6596)
                     /locus_tag="PLZ75_00590"
     CDS             complement(6117..6596)
                     /locus_tag="PLZ75_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOS70925.1"
                     /translation="MSLTSVTGNLPIILSYLGLALMLGLCGIGSSIGVTMGGNATLGA
                     MKKNPDAFGNYMLLSALPGTQGLYGFGGFFVINSSGVINPEMTMLQGIAILTAGLSLG
                     FVGLYSALKQGSVCANGIASIGSGHDVFGNTMILAVFPELYAIIAFAATFIINASIA"
     gene            complement(6630..8624)
                     /locus_tag="PLZ75_00595"
     CDS             complement(6630..8624)
                     /locus_tag="PLZ75_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOS70926.1"
                     /translation="MIAGIKKYAFLVHHKDYSDLLDTLRKAGVVHITEKHRLDEGNVL
                     QVDLERLNRYRSAIRQLRILVPGSAVSEQSEDPETVLAGLESLIKEIEDRKHALERLR
                     AEADRARPWGRFDPSVFRKLDDTDWTVSLFSCSRKHFREEWKGEHAIEVISEKGSRIY
                     FAVIHRKGEFPDLDAGEETIPERSVAAVLDEIKVNEDLLADADARLKLKAPEWNKALQ
                     DGSKNVINRIEYLTVEQQADKYAEDNLYVLEGWVPDDEEERLRKMLEDSDCYFFAVEP
                     SENEKVPIILKNNKFASLFEPITRLYSLPDYRELDLTPFFAPFFMLFFGFCLGDAGYG
                     LFFLIAGFIIKRKVSLKLRPFVTLAQYLGAAAMLFGMLTGTFFGMNLIDTGYTITGQS
                     VIRMEEYGVPAKTVNELKQLKDISFSTRQEFVAEVIKVTGGDIPGAQKNAILKSAEGN
                     FSLIRSFRHLMQDSLSMFYLAIILGAIQILFGMILKVVNISRQKGFRYSLSVIGWIVL
                     LLTLIIFKGGAALKLLDEARVAFLFNTLLIFSLVLIFLLNTPGINVFLRIGNGIWDSY
                     NVITGIFGDLLSYIRLFALGISSSILGFVFNDISSQMLSVPYVGWLFFIVLLLIGHSL
                     NIFMATLGGFVHPLRLTFVEFYKNAGFSGGGLDYKPFKLK"
     gene            complement(8621..9232)
                     /locus_tag="PLZ75_00600"
     CDS             complement(8621..9232)
                     /locus_tag="PLZ75_00600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOS70927.1"
                     /translation="MAVKFQYNKISLQNLNKQLSIRTKALPILKNKEAALRTEVKRAR
                     DEADNFEKELASIVNRYRDGLRLWSEFDSGLLRVEEIKTDFVKIAGITIPEFRDITFT
                     LNPFNLYESPAWFAEGIKILKEMAAALAKRDLMREKMGLLDHARKKTTQKVNLYEKVQ
                     IPGYEDAIRRIKRFLEDEENLSKSAQKIIKARQEELTDKNGTE"
     gene            complement(9245..10579)
                     /locus_tag="PLZ75_00605"
     CDS             complement(9245..10579)
                     /locus_tag="PLZ75_00605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOS70928.1"
                     /translation="MRERAAFQKIYTKLTRITKATCSVKATGAGYDELALVDGRLAQV
                     VKMVGDEVTLQIFSGTEGIPTNAELIFLGRPPALKVNEDLAGRFLNAYGEPIDDGPAL
                     EGMNREIGGPSVNPVRRMQPSKLIATGIAGIDLNNTLVTGQKIPFFADPDQPFNQVMA
                     TVALRAQADKIVLGGMGLTNDDYLFYRNIFENAGALERIIAFINTTENPPVERLLVPD
                     MALTAAEYFAVEKNEKVLVLLTDMTLYADALAIVSNRMDQIPSKDNMPGSLYSDLAKI
                     YEKAVQFPDGGSITIIAVTTLSEGDITHAIPDNTGYITEGQLFLRRDTEIGKVIVDPF
                     RSLSRLKQLVIARQTRNDHAQVMNSAIRLYADAANARTKLENGFELSNYDERTLSFAK
                     EYSEKLLAIDINIDITTMLDTCWSLFGKYFSKEEVAIREEFVKEYWPAESAN"
     gene            complement(10576..12333)
                     /locus_tag="PLZ75_00610"
     CDS             complement(10576..12333)
                     /locus_tag="PLZ75_00610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOS70929.1"
                     /translation="MATKGKVYGIISNLVIVNVDGPVAQNEICMISHDATKLMAEVIK
                     LQGDQAYVQVFESTRGLKIGSEVEFTGSMLEVTLGPGILSRKYDGLQNDLEKLDGIFL
                     EKGEYNFPLDQNAKWEFTPLAEAGSVVEAGSWLGEVMENWIPHKIMVPFKMEGSYRVK
                     STSPKGFYKVNDTIAVITDRDGTEHNITMVQKWPVKIPVRVYRNKTRPFRIMETGVRV
                     IDTLNPIAEGGTGFIPGPFGCGKTVLQHALSKQADADMVIFAACGERANEVVEIFKEF
                     PELTDTRTGRKLIERTTIVANTSNMPVAAREASVYTAMTLAEYYRSMGLKVLLLADST
                     SRWAQALREMSNRMEELPGPDAFPMDLPAIISNYYSRAGYVELVNGSTGSITFIGTVS
                     PAGGNLKEPVTESTKKAARCFYALSQQRSDSKRYPAVDPIESYSKYLEYDEVKDYLSE
                     KISPAWHDQVTKAKNILMRGREAQEQINILGDDGVPLDYHVRFWKSEVIDFIILQQDA
                     FDRIDQSTPLERQRYMLSKVLEVNDRELKFDTFEEVATFFKRIINLFRQMNYSEFESD
                     AFNEYQKELDMQIKEIAVI"
     gene            complement(12414..13286)
                     /locus_tag="PLZ75_00615"
     CDS             complement(12414..13286)
                     /locus_tag="PLZ75_00615"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOS70930.1"
                     /translation="MFKREYHYLVAGLADLVFDSSKGASDIAEFREELKSALHPSDYA
                     VVSILFLPHDNKNLMTYLEGGSGEWDNLGIYSLQDMEEQKRITESILREEDILPPYMV
                     KAIRKWFDSGSANDRFETERILTEGYIDTALASGNRFLMNYVSFDRDMKNVIALISSK
                     ALGLDADDFISGDYPLARRLREIFKSSRDFQIPPEPDYVPDMFRIMTEEEFLERERKI
                     DLARWEFINSETFFDYFSVDWIMGYLIKLSIVTRWKQLDPETGKIMLRNLVEEMKPSA
                     NTVHTAGKQEEEGN"
     gene            complement(13286..13903)
                     /locus_tag="PLZ75_00620"
     CDS             complement(13286..13903)
                     /locus_tag="PLZ75_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70931.1"
                     /translation="MQDKLQEITEKIYQEGVSRGNEEAEKIIAEARKESVRMTEKAEE
                     KAGQIIAAAERKAGEIIKNAESEIKLAFRLSQNSLRQDIENKIAFRIIDEPVSEIFAD
                     NSFLAGLIGIIAEKWAQQGNDEGFEIRMPEEILPDVEKFLEEKLNKTLSDRVTLNPGK
                     SMVKGFEIHPSGREYKISVSDSNMAEFLRDFIRPRLAELLFGEEK"
     gene            14175..15800
                     /locus_tag="PLZ75_00625"
     CDS             14175..15800
                     /locus_tag="PLZ75_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOS70932.1"
                     /translation="MKKLIITSMVLLIFLTRLQAENEINIKAEIAHVTVYPDRAQLSH
                     EAVVDIPSGKTLLKLSGLSPYIDARSIQVKGRGNFTILSVNHQNNYLLNLEDSPEITK
                     IKKQIEALQLKIEDEKAAIGVLKEKEAFLIANRAIPVKESAFSTDQLKNYMQLYTDNM
                     DQVSTSVLKKNRLIKEYEKQIAALQNQLSESSGRQQLHSGEITVSVSAEKALSARMDF
                     AYVVSNAYWFPSYDVRVDDISNPVEISYKANLFQNSGVEWKNVRLSFSNASPWIAGDI
                     PELNPWFIDYYKAPVLTVTARGVASSAPRRLDKAVMDYELVPQENKAEEVPEAVPVPV
                     ERKTGETAVSFDVAIPYTIASDGRIRTIEIQRTSVPAEYKYMTVPKMSPHAYLTGNIG
                     NWAGMSLQSGEATLYFENTFVGKSAINVNQLSDTLTLSLGTDNSILVKREKRKDYTSR
                     RTFGANKTETFSFLISIRNNKTNPVRINVVDQIPVSTNSGITVEALELSGGRHNAASG
                     KISWNYELKPQETREIVLTYSVRYPRDKTVILE"
     gene            complement(15961..16737)
                     /gene="lpxA"
                     /locus_tag="PLZ75_00630"
     CDS             complement(15961..16737)
                     /gene="lpxA"
                     /locus_tag="PLZ75_00630"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOS70933.1"
                     /translation="MISNSAHITPGAVIGNDVTIDPFAYIAADVVIGDRTWVGPGAVI
                     LDGARIGKDCKIFPSSVVSGIPQDVKFRGEITTAEIGDNTIIREGVTVNRGTVTTGKT
                     VVGNNCLLMAYAHVGHDCIIGNNCIIGNACGLAGEVRIDDWAILSGAVLVHQFSRIGG
                     HVIIGGGSKVRTDVPPFIKADRDPLCFMGLNTVGLNRRNFSKERIDEIHNIYRIIYQS
                     KRNTTQALEYVENEFRPSPDRDYILNFIRDSGRGIIRGPR"
     gene            complement(16742..18136)
                     /locus_tag="PLZ75_00635"
     CDS             complement(16742..18136)
                     /locus_tag="PLZ75_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOS70934.1"
                     /translation="MSEKQRTLAKSISLSGKGLHTGVNVRITFNPAPANHGYKFCRTD
                     LPGKPVIDALAEYVSDTSRGTTIQQNNVSVGTVEHVLASFYGLGVDNALIELDGPEAP
                     IMGGSAQKFVEAIKEAGIVEQDEERNYFVIKEKLAYSDEERGVDLIVYPDDHLSINVL
                     IDYNSRILGNQYAILDNIDEFEKNIYNSRTFVFFHELEPLYRNGLIKGGDLDNAVVIL
                     EKEVEQEEIDRIARLFRRPGISKHTAGVLNNTELRYPNEPARHKLLDLLGDLALVGFP
                     FKGKVVATKPGHYANTRLARIIRQEMKKSLSKHKIPFYDPNRPPVYTLEDIKKRLPHR
                     APFLLVDKIISLDETTVVGIKNVTFNESFFQGHFPDEPVMPGVLLVEALAQSGGLLVL
                     SGLDEPERYSTYFLKIDKLKFKQKVVPGDTIILKMELTEPLRRGIVTMYGQAFVGNNL
                     VVEGEMTAQVIKNK"
     gene            complement(18259..19302)
                     /gene="lpxD"
                     /locus_tag="PLZ75_00640"
     CDS             complement(18259..19302)
                     /gene="lpxD"
                     /locus_tag="PLZ75_00640"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOS70935.1"
                     /translation="MEFTAQTIAEFLKGEIAGDPAVTVNSIAKIEEGKKGALSFLANP
                     KYEPYIYKTDSSVVLVNRDFAPSGPVKATLIKVDNAYEAFASLLTLVEQSRPRKKGMH
                     PTAIIEEGTSIGQDAYIGAYVYIGENCRIGNNCRIYPQSYIGDGTIIGDDCLIYQGVK
                     IYHDCVIGNKCTIHAGSVVGSDGFGFAPQSESEFMKIPQIGNVIIEDHVEIGANVTID
                     RATMGSTIIRRGVKLDNLIQVGHNVEIGENTVMAAQTGIAGSTRVGRNCMFGGQVGVA
                     GHLRVADGTRLGAQAGVAGSIKEENHILTGTPAVDHRIFLRSSVIIRNLPELKKQIDS
                     LEREVENIRKDKS"
     gene            complement(19319..20572)
                     /locus_tag="PLZ75_00645"
     CDS             complement(19319..20572)
                     /locus_tag="PLZ75_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOS70936.1"
                     /translation="MVYGTNKRKIINDPVYGFISVPGDFIFDLIEHPWFQRLRNIRQL
                     GLTNLVYPGANHSRFQHCLGALHLMTMAIHTLRGKGTEISAEEEEAALAAILLHDSGH
                     GPFSHSLENSIMSGISHEDISLLLMRKLNEEYGGRLGLAIDMFRNSYSRKFFHELISG
                     QIDMDRLDYLRRDSFFTGVIEGSVGSDRIIRMLNVVNDSLVIDEKGIYSLEKFLIARR
                     LMYWQVYMHKTVIASEKLLINLLRRAKELAVEGRNLYATPALRFFLYNSIGPDDLYRE
                     NEFTPGIIAANFTRLDDSDIFVSAKYWMDSSDRILSDLASRLAERGLYAVELQNKPFS
                     AAKVNRMIEKLTDVMKLDPALAGYYVFSDSISNLAYSAGFPEVKILLKSGETAGISEV
                     SDMFGESFLSERITRYFLCYPKELR"
     gene            20789..21157
                     /locus_tag="PLZ75_00650"
     CDS             20789..21157
                     /locus_tag="PLZ75_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOS70937.1"
                     /translation="MGRYVNNHLIRDEEVVFETRLHWITFLTFKSLFTLTLAAWFARW
                     FSEFVITNRRIIIKTGIISRTTFEMNLSKIESVNVDQSVSARIFNYGSITIIGSGGTR
                     ESFHKIAKPLAFRKAFQELC"
     gene            21281..21817
                     /locus_tag="PLZ75_00655"
     CDS             21281..21817
                     /locus_tag="PLZ75_00655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOS70938.1"
                     /translation="MKDLAIISSSVRSGRLSHRVALYFRNYIEENRLASVRILDLKEY
                     NFPLFDERLKFQTDPSPEVIEFAERIRDADGILIVTPEYNGGYPASLKNVVDLLYEEW
                     HGKPLAIATVSDGHFGGSQVITSLLFSLWKMRVRVVTAMFPVPRVMDAFDENGIPKDK
                     ARTDKRASNFLKELFAAM"
     gene            complement(21932..22798)
                     /locus_tag="PLZ75_00660"
     CDS             complement(21932..22798)
                     /locus_tag="PLZ75_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOS70939.1"
                     /translation="MKTLKSLLISSFLVVFSLSLAGQKSTDKSAAGIIETIIRQTRTA
                     SIPNTVDVIKAGDPGTEVKGIVTTMFATMEVLKKAKELGCNLIIAHEPLYYNHLDDTK
                     QLQNDPVYLEKKKFIDDNKLVVWRFHDYIHSLKPDAIDYGMTLKLGWTDYVSENRYNR
                     FDIPRTSLKELLVYLKRIFPENAFHIIGNPDMKLSGIAFAAGAPGSSAHIRILRDPTV
                     DVLLAGEVPQWETYEYARDAVSQNRNKAVIFLGHVTSEESGMEYCAEWLREFIKDIPV
                     HFVASGPSYWTY"
     gene            22959..24407
                     /locus_tag="PLZ75_00665"
     CDS             22959..24407
                     /locus_tag="PLZ75_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70940.1"
                     /translation="MKKLIFTPILALLIAAGTTASAQDFPEEYLGLPGDNLNLYAVMK
                     LFQESETLEGFERSLNDENSRINNLDLNGDNLIDYITVHDYVDGDVHTIVLRVALSRN
                     EFQDVAVFTVQKFRDGSVNIQLVGDEALYGKNYIIEPIYNETPNPGYTGRASATRRVN
                     VTVVRTTPYEVAAWPVITYIYSPRYVVWRSAWYWGYYPSYWRPWRPYYWHYYYGYHYN
                     WYHYYYNHYHYWPHYRYPRYTAYYYTTVRVHSTVVVNNVRDGYYRTTYSRPEQRREGE
                     ALFTRVNPDQTRRTAENTPAPRMRREVFREGNERDAATGTARRQAEQNGERAATRSSA
                     DRNTGTERRAASTAGDRSAARTAPAQSSSTERRAASTAGDRPAARTAPAQNSSTERRA
                     ASTAGDRPAARTAPAQSSSQARSAPATASQRSAAKPAPAVKSQSSAGTRQSAPAARST
                     QTRPARESRQAGTQERSAGRKESSESSNTRRK"
     gene            24741..25193
                     /gene="rimP"
                     /locus_tag="PLZ75_00670"
     CDS             24741..25193
                     /gene="rimP"
                     /locus_tag="PLZ75_00670"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOS70941.1"
                     /translation="MIDKERIERIINEHITGTDMFLVSLKVSTANRITVLADTMKGIT
                     IDECAELHRFLEKQLDRNIEDFELQVSSPGLDAPFRVIQQYYRNEGNMIELTDMNGEK
                     FTGKLRNVTDGGFELETVIKEKGKSPSVKELSFNYDQIKTAKAVFKVN"
     gene            25214..26458
                     /gene="nusA"
                     /locus_tag="PLZ75_00675"
     CDS             25214..26458
                     /gene="nusA"
                     /locus_tag="PLZ75_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOS70942.1"
                     /translation="MENVNLIDTFSEFKELKNIDRPTMMSVLEDVFRNMLAKKYGSAD
                     NFDIIVNIDKGDFEIWRNRVVVNDDELSDPVTQIPLSEAKKIDEDYEVGEDVSDEVKF
                     LDFGRRAILSLRQNLTARILDLEKNNIYMKYKDRIGEIVTGEVYQVWKREILILDDDG
                     NELILPKSEQIPSDHFRKGDSIRAVVIKVEMRNNTPFIILSRTSPVFLERLFELEVPE
                     IFDGLITIKKIVRVPGERAKVAVESYDERIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INYTTNNQLFIQRALSPAKITTIKLIEEGNTRKAEIFLKPNEVSLAIGKGGLNIKLAS
                     QLTGYEIDVYREPGDEDEEDVNLEEFSDEIEQWIIDELKSIGCDTAKSVLSLSINDLV
                     KRTDLEEEQIREVVNILRAEFE"
     gene            26514..29441
                     /gene="infB"
                     /locus_tag="PLZ75_00680"
     CDS             26514..29441
                     /gene="infB"
                     /locus_tag="PLZ75_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOS70943.1"
                     /translation="MTEEVKVMRLSKAAREFNVGIATIIEFLHKKGFVLDSNPNTKLP
                     PEACLLLEKEYGPDINLKKESEKLALRDLRNKATLTLDDVTGKPQVEEPEREEEILLK
                     DTTTRIDIRTEIKKPAVKLVGKIDLEKTAKTRTEKAEEVKKEKAEKKVSKPAAKDEKP
                     AESTAPEEKKLPTVDLTIVGKIDLEAVSPRRVTPAPEEAVEEIKKEEKPGVISSVDEE
                     KPAAAPAEAETEVAGKAEITAAPAEETEEPMTDQEEVIELFRPDIAKLSGPTVVGKIQ
                     LPAEEKKKSAQQQPVKTDAESEQRRKKRRKRITKEKEQVTVAKADTAEKKEKQARKSP
                     KRRPVRAEVDEEEVQKQIKETLARLTSKGKSRGSRYRREKREAISQKHREFEDRIEQE
                     KNILKVTEFVSVNELAIMMNIPVTDLISTCMNLGLFVSINQRLDAETMVLLADEFGYN
                     VEFVSADLLAAVREEEDEEYELQPRPPIVTVMGHVDHGKTKLLDYIRRTNVIAGEAGG
                     ITQHIGAYAVKLDDQRQITFLDTPGHEAFTAMRARGAQITDIAIIVVAADDGVMPQTV
                     EAINHASAAGVPIVFAINKIDKPTANPDKIKEALANMNYLVEDWGGKYQSQEISAKSG
                     LNVELLLDKVLLEAEILELKANPDKAAIGTVIESSLDKGRGFIATVLVKAGTLRVGDM
                     MLAGSHYGNVRAMYNERGHRVSEAGPSTPALILGLNGAPQAGDQFNVMETEREAKDIA
                     TKRAQLQREQGLRTQKHITLDEIGRRIAIGNFQELNIIVKGDVDGSVEALGDSLIKLS
                     TPEIQLNIIHKAVGQISESDVLLAAASNAIIVGFQVRPSHSARKLAEKEEIDIRLYSI
                     IYDAIEEIRSAMEGMLSPEMKEEIVATLEIREIFKVTKVGTVAGCYVKEGKISRNSSV
                     RVIRDGIVIYTGELGSLKRFKDDAKEVAGGLECGLNIHNFNDIKVGDVIEGFKQIEIK
                     KTL"
BASE COUNT         8037 a         7114 c         6485 g         7928 t
ORIGIN      
        1 gtctttgctc ccgccagcag gaattccggg ttaatcctcc ggggaatatc cctccggagg
       61 aaagacattt ccgcattcat gccggcttca caccattgtt ccagaatcct gccgtcatca
      121 gccagtattc cggccctggc aataccgcag agatcaaatc ccagagcggc agccctgccc
      181 tttatggaag ccgacagccg ttttctttca tccggtccca ttgattcccc tacaaaaggc
      241 ccagttctag cttaacctca tcgctgagca tatccggatc ccaggggggc ataaaagtaa
      301 gtttcagatc caccttttca atgccttcca cttttcccac cttctccctg acctcctcca
      361 ggagatggtc tgccacgggg caattgggat tggtgagtgt catccgtata tgagccacgg
      421 ctccgtcgtc cacccggatt tcataaatca gtccgagatc gtatacgtta gcagggatct
      481 ccgggtcata gacggttctg agactgtcaa tgatccttga ttccacatca atttgctgag
      541 aagaaaccat aaaatcagta ttaattaatt acaagtccgg gccggagggt gtcacaggcc
      601 tttcagacgg ccggaggctg ttttgcgttg aatgctattg catataacct gatctgtttc
      661 accattgaaa gtaatccgtt ggaacgggtt ggtgaaagat tctgatgaag tcctattctg
      721 tcgataaaat acagatccgc tgaaattatt tcagccggag ttctccccga gagaacccgg
      781 atcagcaggg caacaattcc cctggtaatt atagcatcac tgtcggcagt gaaaaatatc
      841 ttcccgtttt caaaggaggg gcacagccat actttcgact ggcatccctc aatcaggtat
      901 tccgtagtct tgcatccggg atcgataacg ggaaggtcgc ggcccatttc gatcagcaga
      961 ttatacttat ccatccagtc gtcaaagact gaaaagtctt ctattattgc atcctgtacc
     1021 tcgttgaccg tcatcttttt atgaaaacat tgatataacc ttctcaatgc cggcacaaag
     1081 cctgtctatt tcttcatgag tgttgtaaaa acacatcgaa gcccttacgg ttccctcaat
     1141 tccaaacctg tccattaccg gctgtgcaca gtgtgttccc gttctgaccg ctattccgag
     1201 cttatccaga atcattccgg tatcatacat atgaatatct ttcagaagaa atgaaatgat
     1261 tgatattttg tgcggactgt ttccgtatat cctcagaccc tttatggaat gcatttgtcc
     1321 cgtggcatat tccagaagct ctctctctct ctgactgata ttatccagtc ccagtgagag
     1381 cagatacccc agtgccctgg caagtccgat tgcgcccggg aaattcacag tgcccgcttc
     1441 aaatttgaat ggcagatcgt tatagctggt cttttcaaat gtcacatttt ttaccatatc
     1501 accacctccc tggtaggggg gaagttcgga aagcagcccg gccctgccgt acagcacgcc
     1561 aatacctgcg ggtccgtata ttttatgtcc cgaaaagaca taaaagtcgc aatccatttc
     1621 cctgacatcg gttacgccat gctgtacacc ctgggctccg tcgataagga caggtatgtt
     1681 catggcatga gcggcaccta ttattctgtc aaccggaaga atcactccca gcgcatttga
     1741 agcctgggta acagcaacca gcctggtctt tttgctgagc agcttcagat actcttcaaa
     1801 tatcagttcg ccgttgtcgt caacaggtat aactttcagg actgcctgtt tccgtctgca
     1861 catcatctgc cagggaacaa tgtttgcatg atgctcaaga tggcttatca gtatctcgtc
     1921 cccccttcca acaaatcgtt ctccgaatga aaatgccact gcatttattg atccggtggc
     1981 tccggaggta aaaataactt cctcactgct gccggcattg ataaaggatc ttaccgcttc
     2041 ccttgcattt tcatattctt ccgacgccct gtcgcttaaa aagtgcacac ccctgtggat
     2101 attactgttg tacagggtaa atacttcagt tgccgcatca atgacacatt ttggtttctg
     2161 ggtagttgcc ccgttatcga gatacaccag gggtttgtcg tatatattcc ggctcagaat
     2221 gggaaaatca gctcttatgg cagaaatatc cttcaccctc ttcctctttt atccgcagtt
     2281 gatcatacag taagcgcatc tggaaagctc acccttcagg cgctgcatca ccaggctgtc
     2341 aattctgtcg cgcagggctt ccaccctgat attttctatg acctcatgtg caaagccaaa
     2401 catcagcatc agcctggctt catgcctgcc tattcctctc gactgaagat agaagagggc
     2461 atcattgtcc ggctggccta ctgttgcgcc atgactgcat ttcacatcat cggcatatat
     2521 ctcaagctgc ggctttgtgt ccattcttgc atcatcgttc agaagaatat tgttgttctt
     2581 ctgataggcc agggttccct gtgcattctt atcgacaaat attctgccgt taaaggctcc
     2641 cgtagccata tcatcgagta ttcctttgaa gagctgattg ctggtgcagt aaggctgtgc
     2701 gtgaatcaca ttcacaaaat tatccacatg ctggaattta tccgcaagaa acagtccgta
     2761 ggaattgcat tcggctcttt cgcctcccag gtaatgatgg gtgctgttcc ttaccagtcc
     2821 gccgtgaagg gttatgttat ttgatgaggc atggctgttc cgttcctgat gtatgaaggt
     2881 gtgggttatt tttgcagcat tgttgtgttc attctggaca cggatgatgt caaagtgtga
     2941 attttcgccc acgtaaactt cagttaccgc atttgtgagg aatttcctcg gtgacaatgt
     3001 atgatcacag attatcaggg ttactgaagc atttttctcc acaataacca ggtttctgtg
     3061 ctgattgaaa atgtcatcgt ctgattccac gagattgatg atctggatgg gcttgtcggg
     3121 ttcaactcct tccggaacat aaataaagac accgtcggag gcaagggcgg tattgagata
     3181 aaccagaccg tctgaattgc cgttgacata tttcccgtaa tgcttttcaa tctgatcgct
     3241 gaacagtttt gaggcttcat tgagacttcc gatcagaaca cccccgggca acttccgtat
     3301 cttgtccgtt atggtgggat aaaaaccgtt aagcagaaca atggcatgca cgtccatgtc
     3361 ggtcacgtcg catctgaatt cctctgccct tatgaaatcc tgctcaccgg gtatgaaata
     3421 attactgtaa tcatggctga aaaaaggatc cagattggtg tatctgtatg attcgttctt
     3481 tctggtggga atgcccagcc tgacaaattc gcggatcgcg gattcacgga atgaatttat
     3541 atactgggaa gagccggaag atatcctctc aatattgtcc ctgtacaggg ccgaatattt
     3601 atcatattta acggtactat tattcataca cagtactttt atcagcttct ttttttatcc
     3661 agtcatatcc tttttcctca agctcgaggg caaggtccct ccctcccgtt ttaactatcc
     3721 gtccgtcata gagtacatga acaatatcag gtatgatata atccagcagc ctctggtaat
     3781 gtgtgatcac aatcaccgat ctttcaggat gcctgagctt gttgatcccg ttggcaacaa
     3841 tcctgagagc atcgatatcc agtcccgaat cggtttcatc aagaatagcc agtttcggtt
     3901 caagcattgc catctggaaa atctcgtttt tcttcttttc accgcctgaa tatccttcat
     3961 tgactgatct gttggcaagt tttgaatcaa tttcaacaag ttccttcttt tcacgcataa
     4021 gcttcaggaa atcagatgcg gaaagaggtt caagccctct gtgcttgcga tgttcattaa
     4081 cagctgtctt catgaagttg accatgctta ctccgggtat ctcggtcgga tactggaaac
     4141 ccagaaatat accttccctt gaacgttcct ccggaggcat ttccagaagg ttctttccca
     4201 tgtatgtaac agtgccttcc agtatattgg cgaactccct ccctgcaagg atggctgcaa
     4261 gtgtgctttt ccccgacccg ttaggcccca taagggcatg tacctcaccc gccttcagtt
     4321 cgagattgat acccttcagt atggtctttc cgtttatact tgcttttaaa ttattgatag
     4381 ttagcataat ataatttaat ttttaatagt ctcagccgac acttccctcc aggcttatct
     4441 gtaaaagttt ctgagcctcc acggcaaatt ccatcggcag cttgtttatt atctcctttg
     4501 catatccgtt tacgatcaga ccaatggcat cctcggtaga tattcccctc tgattgcagt
     4561 aaaaaatctg gtcctcccca atcttcgagg ttgttgcttc gtgttccact attgccgaaa
     4621 ggttgtcaac ctccaggtag ggaaaagtat gcgcaccgca tttatctccc agcagaagtg
     4681 aatcgcactg tgaaaaattc cgtgcgcctg aagccttcct gtgaactttc accagtcccc
     4741 tgtagctgtt ctgactgaaa cccgcggaga tgccctttga tactatggtg cttctggtat
     4801 ttttcccgat atggatcatt tttgtgcctg tatcggcctg ctgatgattg tttgtcaccg
     4861 ccactgaata gaattccccc gtggaattat cgccccggag aatacaggaa gggtacttcc
     4921 aggttattgc agatccggtc tcaacctgag tccaggaaat ctttgagttt tcccccctgc
     4981 acaaacctct ttttgttaca aagttgaaga tacccccctt cccttccctg tcgccagggt
     5041 accagttctg aacggtggaa tattttactt cagcattttt ttcggcaata atttcaacta
     5101 tggcggcatg cagctgattc tcatccctca tcggggcagt acagccctca aggtagctga
     5161 catagctctc ctcgtcggca atgatcaggg tgcgctcaaa ctggcccgtg ttggcagcat
     5221 tgatcctgaa atatgttgaa agctccatgg ggcatctgac tcctttcgga atgtagcaga
     5281 atgagccgtc actgaacacc gcggaattat gtgccgcaaa aaagttatcc gcatagggaa
     5341 ccaccgtgcc catatatttt ctgaccagat caggatgttc cttcaccgca tcactgaaag
     5401 aacagaagat tataccggct tcggccaggg tttccctgaa agtggtcttc acggaaacac
     5461 tgtccatcac agcatcaaca gcgatcccgg caagatgctt ctgttcctca agagggatgc
     5521 ccagcctgtc aaaggtccgc ttcagttccg ggtcaatctc gtcattgctt cctgaggtct
     5581 ttctctgctt cggggcagca taatacacta tgtcctgata gtcgatttcc ggtatggtca
     5641 gattggccca ttccggcatt ttcatggtct gccagcgcct gaatgccttg aggcgaaact
     5701 caaggagcca gcctggttct cccttttttt ccgaaataat acggattgta ttttcattca
     5761 gtcctttcgg aatcttctca atctcaatat cggagtaaaa tccatatttg tactcccccg
     5821 aagtaacatc atttaatatc tgatcctggt ttttttccat ctcactgatt cttctctctt
     5881 tctatcatgt taacaaagcc ggagtgcaaa tgttaattgt atttaatcta aataaagtct
     5941 ggcaaaatta ttaaaaaaaa caattcaggc gattatcatc cgggaaaact aatgccggca
     6001 ttgccggcac aagtatgttc cgatcggata taagggcatt tccgctctgc cgtgaataca
     6061 ccggtcccgt gaggccggcc ccaggggccg gaattctcac tgaacaactg aaattgtcag
     6121 gctatggaag catttatgat gaaggtggca gcaaaggcaa taatggcata gagttctgga
     6181 aacactgcca ggatcatggt attaccgaaa acatcatgac ctgatcctat tgaggcaatg
     6241 ccgtttgcac agacactccc ctgtttcagt gcggaatata atcccacaaa gcccagggac
     6301 agtcctgcag taagaattgc aataccctga agcattgtca tttccggatt tatgacaccg
     6361 ctgctgttta taacaaagaa gcctccgaaa ccataaaggc cctgagttcc gggaagagca
     6421 ctcagaagca tgtaattacc gaaagcatca ggattttttt tcattgcccc cagggtggca
     6481 tttccgccca ttgtcactcc tattgagctg ccaatgccac acagtccgag catcagagcc
     6541 agccctagat atgatagtat gattggcaga ttccctgtaa ctgaagtaag actcattgtt
     6601 tccataatag atatttggtt aatcgtttat tattttaatt taaaaggttt ataatcaagg
     6661 cctccgccac tgaatccggc gttcttgtaa aactccacaa atgtaagcct cagcggatgt
     6721 acaaatcctc ccagtgttgc cataaaaata ttgagggaat ggcctataag gagcaaaaca
     6781 ataaaaaaga gccagcctac atatggtact gacagcatct gtgaagaaat atcattgaat
     6841 acgaatccca ggatggacga agagattccc agtgcaaaaa gccttatata ggacaggagg
     6901 tcgccgaaga tacctgttat cacattgtag gaatcccaga ttccgtttcc tattctcagg
     6961 aaaacattga ttcccggtgt gttcagaagg aatatcagca caagggaaaa gattaacagg
     7021 gtgttaaaca ggaaggcaac ccttgcttca tccagcagtt tcagtgctgc ccctcccttg
     7081 aaaattataa gtgtcagcag cagtactatc catcctataa cggaaaggct gtatctgaat
     7141 cccttctgcc tggaaatatt aacaactttc agaatcattc cgaaaaggat ctgtatggct
     7201 cccaggataa ttgccagata gaacatgctt aatgaatcct gcataagatg tctgaaagat
     7261 ctgatcagtg aaaaatttcc ctcagcgctt ttcaggatgg catttttctg tgcaccggga
     7321 atatcaccgc ctgttacttt tatcacttcc gcgacaaatt cctgtctggt ggaaaatgat
     7381 atatccttta attgtttcag ctcattcaca gttttcgccg gaacgccgta ttcctccata
     7441 cggatgactg actggccggt aatggtatat ccggtatcaa taaggttcat tccgaaaaat
     7501 gtgcctgtaa gcattccgaa cagcatggca gccgctccca ggtattgtgc cagcgtaaca
     7561 aagggcctca gtttcaggct gacttttctt tttattataa atcccgctat aaggaagaag
     7621 aggccatagc cggcatctcc caggcagaat ccaaagaaaa gcatgaaaaa gggagcaaag
     7681 aagggggtaa gatcaagttc cctgtagtcc ggcagggaat acagtcttgt tatcggttca
     7741 aaaagactgg caaacttatt attcttaaga attatcggaa ctttttcatt ttccgaaggt
     7801 tccaccgcaa agaagtaaca atctgaatct tcaagcattt tcctcagcct ctcttcttca
     7861 tcatcgggta cccatccctc gaggacgtaa agattgtctt ccgcatattt atcagcctgc
     7921 tgctcaactg taagatattc tatcctgttg attacgtttt tgctgccatc ctgaagggct
     7981 ttgttccatt ccggtgcttt cagttttaaa cgcgcatcgg cgtcggctaa aagatcctca
     8041 ttcaccttta tttcatccag gacagctgca acagaccgtt cgggtattgt ttcctccccg
     8101 gcatccagat caggaaattc acccttcctg tgaataacgg caaaatatat ccggcttccc
     8161 ttttcactga taacctcaat ggcatgttct cctttccatt cttccctgaa gtgttttctt
     8221 gaacagctga agagggagac agtccagtcc gtatcgtcca gcttcctgaa aaccgacgga
     8281 tcaaacctgc cccacggcct tgccctgtca gcctctgccc tgagtctttc aagagcatgc
     8341 ttccggtctt caatttcctt tatcagcgat tccagtccgg cgagcaccgt ttcaggatct
     8401 tccgactgtt cagaaactgc tgaacccggc accagtattc tgagctgcct gattgccgac
     8461 ctgtacctgt tcagacgttc gagatcaacc tgcaatacat tgccctcatc aagtctgtgt
     8521 ttttcagtta tgtgaaccac acctgccttc cgcaaagtat ccagcaaatc agaataatcc
     8581 ttgtggtgaa ccagaaaagc atattttttt atccctgcaa tcattcagtt ccgtttttat
     8641 ctgtaagttc ctcctgacgg gccttgatta ttttttgtgc cgacttggaa aggttttcct
     8701 catcttccag aaagcgtttt atccgcctga tggcatcttc atagcccggt atctgtacct
     8761 tttcatacag gttcaccttt tgagtggttt tttttcttgc atgatccagc agccccatct
     8821 tttccctcat cagatccctt tttgcaaggg ctgcagccat ttccttcagt atcttaatcc
     8881 cttcggcaaa ccaggccggg ctttcataaa gattaaaagg attcagtgta aaagtaatgt
     8941 ccctgaattc aggaatggtg ataccggcta ttttaacaaa atcagtcttt atttcctcca
     9001 ctctcagcaa acctgaatca aattccgacc acaggcgcag cccatcccgg taacggttga
     9061 caatggatgc gagttccttt tcaaaattat ctgcctcgtc cctggcacgc ttcacttcag
     9121 tcctcagggc tgcctccttg ttcttcagaa tgggcagagc cttagtcctg atactgagtt
     9181 gcttgttgag attctgaaga gatattttat tatattgaaa tttgacagcc ataaacagaa
     9241 attcctaatt tgctgattcc gcaggccagt attcctttac aaattcctcc ctgatcgcaa
     9301 cttcttcctt tgaaaaatac tttccgaaaa gactccagca ggtatcaagc atcgttgtaa
     9361 tatcaatatt gatatctatt gccagaagtt tttccgaata ttcctttgca aagctaagag
     9421 tcctttcatc gtagtttgac agttcaaaac cgttttccag cttggttctt gcatttgcag
     9481 catcggcata aagcctaatc gcactgttca tcacctgtgc atgatcattc cgtgtctgcc
     9541 tggctatgac aagctgtttc agcctcgaaa gcgagcggaa cgggtcaaca attacctttc
     9601 ctatttcagt atcgcgcctg agaaaaagct gtccttcggt aatatagccg gtattgtcag
     9661 gaatggcatg ggtgatgtca ccttctgaaa gtgttgttac cgcgatgatg gttattgaac
     9721 ctccgtcagg aaactgaacg gctttttcat aaatctttgc aaggtcactg taaagtgaac
     9781 cgggcatatt atcctttgaa ggtatctgat ccattctgtt tgacactatt gccagtgcat
     9841 cggcatacag cgtcatatca gtcaggagca cgagcacctt ttcatttttt tccactgcaa
     9901 aatattccgc ggcagtcagt gccatgtccg gcaccaggag tctttcaacg ggcggatttt
     9961 ctgttgtgtt tataaatgca attattcttt caagagcacc tgcattctca aaaatattcc
    10021 tgtaaaaaag gtaatcatcg tttgtcaggc ccattccgcc gagaacgatt ttgtcggcct
    10081 gagccctcag ggccacagta gccattacct ggttgaaagg ctggtcggga tctgcaaaaa
    10141 acggtatctt ttgtcccgta acaagtgtat tgttcaggtc aatccctgca attcctgtcg
    10201 ctatcagctt tgaaggctgc atcctccgta ccggattgac tgacgggccg ccgatttccc
    10261 ggttcatacc ctcaagggca gggccgtcgt caatgggttc accataagca ttcagaaatc
    10321 gtccggccag gtcttcattc actttcagtg caggaggcct gccaagaaat atcagttccg
    10381 cattggtggg tatcccttca gttcccgaga atatctgaag cgtgacctca tctccgacca
    10441 ttttgacaac ctgggccaga cggccgtcta caagtgccag ttcatcatat cctgcacccg
    10501 ttgctttcac ggaacaagtc gcctttgtaa tacgtgtcag tttcgtatag atcttctgaa
    10561 atgctgctct ttctctcata ttactgcaat ttctttaatc tgcatgtcaa gttctttctg
    10621 atattcattg aatgcatccg attcaaattc cgaataattc atctgtctga aaaggttgat
    10681 tatccttttg aagaaggtag ccacttcttc aaaagtatcg aattttaact ccctgtcatt
    10741 gacctcaagt accttgctga gcatgtagcg ctggcgttcc aggggagtgc tctggtcaat
    10801 cctgtcaaag gcatcctgct gcagaataat aaaatcaatg acttcggatt tccagaaccg
    10861 gacgtgataa tcaagcggta ccccgtcatc ccccaggatg ttgatctgtt cctgtgcctc
    10921 ccgccccctc atcagaatat tcttcgcctt ggtcacctga tcatgccagg caggggatat
    10981 cttttccgac aggtaatctt taacctcatc atattcaagg tatttagaat aactttctat
    11041 aggatcaact gcggggtatc ttttagaatc ggacctctgc tgcgagagtg cataaaagca
    11101 tcttgccgct ttttttgttg attcggtcac gggttctttc aggtttcctc cagccggtga
    11161 aacggttccg ataaaggtta ttgaaccggt actgccgttt accagttcca catagccggc
    11221 cctggaataa tagttggaaa tgattgccgg gaggtccatc gggaatgcat ccggtccggg
    11281 cagttcctcc atccggttcg acatttccct gagggcctgg gcccacctgg aagtggaatc
    11341 ggcaagcaaa agcaccttca gtcccattga ccggtagtat tcggcaaggg tcattgccgt
    11401 atagacggag gcttcccggg cagccaccgg catgtttgaa gtgtttgcca caatagttgt
    11461 tctctctatc agctttctgc ctgtcctggt gtcggtcagc tcaggaaact ccttgaatat
    11521 ttcaacaact tcatttgccc tctcaccgca ggcagcaaaa ataaccatgt cagcatcggc
    11581 ctgtttggaa agcgcatgct gaagaactgt cttgccgcag ccgaaaggtc ccgggataaa
    11641 gccggttcca ccctctgcta tcgggttaag agtatcaatc actcttactc cagtttccat
    11701 gattctgaat ggtctggtct tattgcggta aacccttacc ggtatcttga ccggccattt
    11761 ctgcaccatt gtaatattgt gttcggttcc gtccctgtca gttataacag caatggtatc
    11821 atttacttta taaaatcctt tcggactggt tgattttacc ctgtagctgc cttccatttt
    11881 gaagggaacc attattttgt ggggtatcca gttttccata acctcaccaa gccaggaacc
    11941 ggcctccaca acacttcccg cttcagccag gggtgtaaat tcccacttcg cattctggtc
    12001 gagagggaaa ttatattcgc ctttttccag gaaaatacca tcaagttttt caagatcatt
    12061 ctgcaagccg tcatatttcc gtgacagtat gccggggccc agtgtcacct caagcatgct
    12121 tcccgtgaat tcaacctcag agcctatttt caatcccctc gtactttcga aaacctgcac
    12181 atatgcctga tcgccctgca gtttgatgac ttcagccata agctttgtcg catcatggct
    12241 gatcatacat atctcattct gggcaaccgg tccgtcaaca ttgacgatga ccagattaga
    12301 tattatcccg tatacctttc cttttgtcgc catttatttc aattacatga tttatataaa
    12361 gtttcatcat aatcctgatt ttcccgaagc cgctttaatc cggcaatacc ttattagttt
    12421 ccctcttctt cctgcttccc tgctgtatga accgtatttg cagagggttt catttcttcc
    12481 accagatttc tcagcatgat ttttccggtt tcgggatcca gctgcttcca tcttgttaca
    12541 atagaaagct ttatcagata tcccataatc cagtcaacag agaaatagtc aaaaaaggtt
    12601 tccgaattga tgaactccca tctggcaaga tcaattttcc tttctctttc aaggaattct
    12661 tcctccgtca taatcctgaa catatccgga acataatccg gttccggcgg tatttgaaag
    12721 tcccggctgc ttttgaatat ttccctgagc cgccgtgcaa ggggataatc accggaaata
    12781 aaatcatctg catccagccc cagggctttt gaactgatta atgcaatgac atttttcata
    12841 tccctgtcga agcttacata attcatcaga aaccggttcc ctgaagcaag tgcggtatca
    12901 atataaccct ctgtcaggat tctttccgtc tcaaatctgt cattcgcact gccggaatca
    12961 aaccatttcc tgattgcctt aaccatgtaa ggcgggagaa tatcttcttc cctgagaatg
    13021 gattccgtta ttctcttttg ttcctccata tcctgcagtg agtatatgcc cagattatcc
    13081 cattccccac tgcctccctc aagatatgtc atgaggtttt tgttgtcgtg cggcaggaat
    13141 agtatggata ccacggcata atcagaagga tgaagggcag atttaagttc ttcccggaat
    13201 tcagcaatgt ctgaagcacc cttgctgctg tcaaaaacaa gatcggcaag cccggctacc
    13261 aggtagtgat attctctttt aaacattatt tttcttcacc aaaaagaagt tctgccagcc
    13321 tgggtctgat aaaatccctg agaaactctg ccatattgct gtcgctgaca cttatcttgt
    13381 attcccggcc tgagggatga atttcaaaac cttttaccat tgatttcccg gggtttagtg
    13441 taacccggtc cgataatgtt ttattcagtt tttcctcaag gaatttttca acatccggca
    13501 gaatttcttc aggcatgcgg atttcaaaac cctcatcatt cccctgctgt gcccatttct
    13561 cagcaataat tccgattaat cctgccagaa aactattgtc ggcaaaaatt tctgaaacag
    13621 gttcatcaat aatcctgaaa gcaattttat tctctatatc ctgcctgagg gaattctgcg
    13681 acagtcgaaa agccagtttt atctccgatt cggcattctt gattatctcg ccggcttttc
    13741 tttccgccgc ggcaataatc tgccctgcct tttcctctgc tttctctgtc attcttaccg
    13801 attccttccg cgcttccgca atgatttttt ctgcttcctc attgcctctg gaaacacctt
    13861 cctggtaaat tttttctgtt atttcctgaa gtttatcctg catgtttctt ctttgttagg
    13921 gaccagtgat ttttgtctgt aaaaaaacag cattcctgat taaaagcata ataataaaga
    13981 aattatgtat ctttaatcag atcctgcacc tgaaaaatat catggatttt aaacaaatct
    14041 tttcaaaggc agcaaaattg caatattttt tcctaatggc aaaacgatct tctgctcgcc
    14101 ggtgttattt tttggtttat atgatattgc acgctttttg ctatgaatat aattcttgat
    14161 tcaaactttt atcaatgaaa aaattaatta tcaccagtat ggttctgctg attttcctca
    14221 cccgacttca ggcagaaaat gaaataaaca taaaagctga aattgcccac gtgaccgttt
    14281 atcccgacag ggcccagctg tctcatgagg cagttgttga cattccctca ggaaaaacat
    14341 tgctgaaatt atcaggattg tcaccctaca ttgatgcaag aagcatccag gtaaagggaa
    14401 ggggtaactt taccatcctt tcagtcaatc accagaacaa ttatcttctg aaccttgagg
    14461 attcgccgga gatcacgaaa ataaaaaaac agatcgaagc ccttcagtta aaaattgagg
    14521 atgaaaaggc agcaattggc gttctgaagg aaaaagaagc ctttctgata gccaacaggg
    14581 caatccccgt aaaggaaagc gcattttcca ccgaccagct taagaattac atgcagcttt
    14641 acactgataa tatggatcag gtgagcacgt cagtactcaa aaagaaccgg cttataaagg
    14701 agtatgaaaa acagatagcc gcattgcaga atcagttatc agaaagttcc ggcagacagc
    14761 agctgcattc aggcgaaata accgtttccg tcagtgcgga gaaagccctt tcggccagaa
    14821 tggattttgc ctatgtggtg tccaatgcat actggtttcc gtcatatgat gtgagagttg
    14881 atgatatttc aaatcccgtt gaaatatcat acaaggcgaa tcttttccag aacagcggcg
    14941 tggagtggaa aaatgtaaga ctgagttttt caaatgccag tccgtggatt gcaggtgaca
    15001 tccccgaact caatccgtgg tttatagatt attacaaagc ccctgtactg accgtgacag
    15061 ccaggggggt tgcttcatcc gctcccagga ggctggataa ggcggtaatg gactatgaac
    15121 ttgttcctca ggagaacaag gcggaagaag ttccggaagc agtgcccgta cctgttgaaa
    15181 gaaaaacagg tgaaacagct gtttccttcg atgttgctat tccctataca atagcatcgg
    15241 acggcaggat ccgtacaatt gaaatacaaa gaacatcagt tcctgcagaa tacaagtaca
    15301 tgacagtacc aaaaatgtcg ccgcatgcct atctgacggg caacatagga aactgggccg
    15361 gaatgagcct tcagagcggc gaggcaacac tttatttcga aaataccttt gtgggcaaat
    15421 cggcaatcaa tgtgaaccag ctttccgata cccttacact ttcgctcggc acggataaca
    15481 gcatactggt aaaaagggaa aaaagaaagg attataccag cagaagaaca ttcggtgcca
    15541 acaagaccga aaccttttca ttccttataa gcataaggaa caacaaaaca aatcctgtca
    15601 gaatcaatgt ggtggatcag attcccgttt caacgaacag cggtatcacg gttgaagccc
    15661 ttgagctgtc aggaggcagg cataatgccg caagcgggaa gatcagctgg aattatgagc
    15721 ttaaacctca ggaaaccagg gaaatcgttt tgacctattc agtcaggtat cccagagaca
    15781 aaaccgtaat ccttgaatag tattgttact gccggttgaa actgtaatca cggaataaaa
    15841 cagctacagg cggattgcga tgttccggca accttgtaat tatattttca gaaaatgaga
    15901 acacccggca atgttatctg gcttattccc gggggactga taattgccct gcggtagtat
    15961 tcagcggggt cccctgatga tacctctccc ggaatcccgg atgaagttca ggatataatc
    16021 cctgtcgggt gagggtctga attcattttc aacatattcc agggcctgtg tcgtatttct
    16081 tttgctctgg tatattatac ggtaaatatt gtggatctca tcaattcttt ccttgctgaa
    16141 atttcgccgg ttaaggccaa cggtgttcag gcccataaaa cagagcggat ccctgtcggc
    16201 cttgataaag ggcggaacat ctgtcctgac tttcgatccg cctccgatta tcacatgacc
    16261 gccgatcctt gagaactgat gaacaagtac tgccccgctc agaatggccc agtcgtctat
    16321 tctgacctca ccggcaagac cgcatgcatt tcctataata caattgtttc cgataatgca
    16381 gtcatggccc acatgggcat atgccatcag caggcaattg ttcccgacca cggttttacc
    16441 agtggtaacg gttccccggt tcacggtcac accttccctg atgattgtat tatccccgat
    16501 ctctgcggtg gtgatctccc ccctgaattt cacatcctgg ggaatacccg aaacgaccga
    16561 tgacggaaat attttgcaat cctttcctat ccgggctcca tcgagtatta ctgctccggg
    16621 acccacccag gttctgtcgc ctattaccac atcagcagct atataggcaa agggatctat
    16681 tgtaacatca ttgccgatga cggctcccgg ggtgatatgg gctgaatttg aaatcattga
    16741 ttcatttgtt ttttattacc tgcgcagtca tttccccctc aaccacgaga ttattgccga
    16801 cgaatgcctg tccgtacatc gtaacaattc cccgcctcag gggttccgtc agctccattt
    16861 tcagaatgat ggtatctccg ggcactactt tctgcttgaa tttcagttta tcaattttaa
    16921 gaaaatatgt tgaatacctt tcaggttcat ccagtccgct gagtaccagt aatcctcctg
    16981 actgtgcaag tgcctcaacc aggagcacac cgggcatcac gggctcatcc gggaaatgac
    17041 cctggaaaaa tgattcattg aaagttacgt tttttatgcc cacaaccgtt gtttcatcaa
    17101 gggaaataat tttatcaacc agcaggaacg gagccctgtg aggcagtctc ttcttaatgt
    17161 cctcgagtgt atagaccggg ggtctgttgg gatcataaaa agggatctta tgctttgaaa
    17221 gtgatttttt catttcctgc cttataatcc ttgcaaggcg tgtattggca tagtgaccgg
    17281 gctttgtggc aactaccttg cccttgaagg ggaaacccac cagggccagg tctccgagca
    17341 gatcaagcag tttgtggcgg gcaggctcat tgggatatct gagctctgta ttattgagta
    17401 ctccggcagt atgtttgctg ataccgggac gcctgaacag cctggctata cggtcaatct
    17461 cctcctgctc cacctccttt tcaagtatca ccactgcatt gtccaggtct ccgcccttga
    17521 tcaggccgtt cctgtaaagg ggttcaagtt catgaaagaa cacaaatgtc ctgctgttgt
    17581 aaatattttt ctcaaattca tctatgttgt cgagaatcgc atactggttg cccagtatcc
    17641 gggagttgta atcgataaga acattgatac tcagatgatc atcgggatag acaatcagat
    17701 ctaccccgcg ttcctcgtcc gaataggcca gcttttcctt aatgacaaaa tagttgcgct
    17761 cctcatcctg ctcaactatt cccgcttctt ttattgcttc cacgaatttc tgtgccgatc
    17821 cgcccatgat cggagcttcc ggaccgtcaa gctcaatgag ggcattgtca accccgagcc
    17881 cgtaaaatga agccagcaca tgttccaccg tgcctaccga tacattgttc tgctgaatgg
    17941 tggtgcctct ggatgtatct gacacatatt cggccagtgc atcaatgaca ggttttccgg
    18001 gtaagtcagt ccggcagaat ttatatccgt ggtttgccgg cgcaggatta aaggtaatcc
    18061 ttacatttac cccggtatgc aatccttttc cgctgagact gatggatttt gcaagagttc
    18121 gctgtttctc actcatcaca acaaaaatta tatttatacc ggctacaaaa gaagcaaaaa
    18181 atcctgaact atcagcaaaa ttcacagtta tgcataaggg ttcgggcaaa tgtcttctgc
    18241 aaaattgtta taaagtcatc atgacttgtc ctttctgata ttttcaactt ccctttcaag
    18301 ggaatcaatt tgttttttaa gttccggcag atttctgata atcactgaag acctcaggaa
    18361 aatcctgtga tcaaccgcag gggttcctgt taaaatatgg ttctcctcct taatactgcc
    18421 tgcaacacca gcctgtgccc cgagcctggt accgtcagca actctcagat gtccggcaac
    18481 gccaacctgg cctccaaaca tacagttcct gcctaccctg gttgatccgg caattcctgt
    18541 ctgtgcagcc ataacagtat tctctcctat ttcaacattg tgtcccacct gtatcaggtt
    18601 gtccagcttc actcccctgc ggattattgt ggatcccatg gtagccctgt caatagttac
    18661 atttgcacct atttcaacat ggtcctcaat tatcacattc ccaatctggg gaattttcat
    18721 aaactcactt tccgattggg gggcaaagcc aaaaccgtca ctgccaacca ccgatccggc
    18781 atgaatagtg catttatttc ctataacaca gtcgtgatag attttcacac cctgataaat
    18841 gaggcaatca tctcctatta tggtgccatc acctatgtag gactgcggat atatccggca
    18901 gttgtttcct attctgcagt tttcacctat gtacacatag gcacctatat aggcatcctg
    18961 gcctatgctt gttccttcct cgatgattgc ggtgggatgc ataccctttt ttctcggccg
    19021 cgactgttcc acgagagtca gaagggaagc aaatgcctca taggcattat ccacttttat
    19081 aagcgttgcc ttcactggtc ccgatggtgc aaaatcgcgg ttaacaagca ctaccgatga
    19141 atcggtttta taaatataag gttcgtattt cggatttgcc agaaaggaaa gagcaccctt
    19201 tttcccctct tcaatctttg ctattgaatt gactgtcaca gcaggatcac ctgctatctc
    19261 gccctttaaa aattcggcta ttgtctgagc tgtaaattcc attctgtaaa ggtgttaatt
    19321 atcttaattc ttttggatag catagaaaat acctggtgat tctttccgaa agaaagcttt
    19381 cgccaaacat atcggacact tccgatattc cggctgtttc tcctgacttc agcaatattt
    19441 tcacctcagg gaagcccgcg gaataggcca ggttggaaat gctgtcagaa aaaacgtaat
    19501 atccggcaag ggccggatca agcttcatca cgtcggtcag tttttcaatc atcctgttca
    19561 ccttcgcggc tgaaaagggt ttattctgca gttccaccgc ataaagcccc ctttctgcaa
    19621 gacgtgatgc aagatccgac aaaatcctgt cggaactgtc catccagtat ttcgctgaaa
    19681 caaatatatc agaatcatca agcctggtga agtttgcagc aataattccc ggtgtgaatt
    19741 cattctccct gtaaagatca tcggggccga tgctgttata gagaaaaaac ctcagggccg
    19801 gtgttgcata aaggtttctt ccctctacag ccagctcctt tgccctcctg agaagattta
    19861 taagaagctt ttccgaggca ataaccgttt tatgcatata gacctgccag tacataagcc
    19921 ttcttgctat aaggaatttt tccagggaat agataccttt ttcatcaatc acaagggaat
    19981 cattcacaac attcagcatc cggataatgc ggtccgatcc gactgaacct tcaatcactc
    20041 cggtaaagaa actgtccctg cgaagataat caagcctgtc catatcaatc tgcccgctga
    20101 tgagctcatg aaaaaacttc cgcgaatagc tgttccggaa catatcgatt gcaagaccca
    20161 gcctcccgcc gtattcttca ttcagcttcc tcatcagcag aagcgagatg tcttcatggg
    20221 atatgccgct cataatagag ttttccaggg aatgagaaaa gggtccgtgt ccgctgtcat
    20281 gcagcaggat ggcggccagt gcggcttctt cctcctccgc cgatatttcc gtacccttgc
    20341 ctctgagggt atggatagcc atagtcataa gatgcagggc acccagacaa tgctgaaacc
    20401 tgctgtgatt cgcacccggg taaacaagat ttgtaagccc cagctgcctg atattgcgaa
    20461 gcctctgaaa ccatggatgt tcaatgagat caaatataaa atccccggga acactgataa
    20521 acccgtaaac cggatcattg attatcttcc gcttgtttgt cccgtacacc acttcggtaa
    20581 ttgtcagcct gtaaaaatat gaattatgtt tgtatccggc aggtttttcg ctgacctgtt
    20641 tctggaatca gatccgggga gcacttaaaa ttaccggaca ccctgatgaa acagtcaccc
    20701 acaagtgatt aacggtctgt gaaaaactta tattgctttc attgctttgt ttgtatattt
    20761 gtcacaacac cattttaatt tacttctcat gggccgctat gtaaataatc atctgataag
    20821 ggacgaggaa gtagttttcg aaacccggct ccactggatc actttcctga cattcaagtc
    20881 attgtttacc ctcacactgg cagcatggtt cgccaggtgg ttttcggaat ttgttataac
    20941 aaaccgcagg ataataatca agacgggcat aatcagcaga acaacattcg aaatgaatct
    21001 gtcgaagatt gaaagcgtga atgtggatca gtctgtatcc gcacgcatct ttaactatgg
    21061 ctccataacg ataatcggat cgggcggcac cagggaatca tttcataaaa tagctaaacc
    21121 ccttgctttc agaaaggctt tccaggagct ttgctgacgc gctttgcggg acgagcccgt
    21181 tgaagtcaac cgagtctgcg gacccggcga aaccagccat gaagtttatc ccggtaaacc
    21241 gggacaggta atctgaacat aaaaattaaa ttacatacag atgaaagatc ttgcaattat
    21301 atcatcaagc gtgagaagcg gcaggttaag ccacagggtg gcattatatt tcaggaacta
    21361 cattgaagaa aacaggctgg cttctgtaag gattcttgac ctgaaagagt ataacttccc
    21421 tctctttgac gaaaggctta aatttcagac agatccttcc cctgaagtga ttgaattcgc
    21481 agaaaggatc agggatgccg acggaatcct gatagtcacg cccgagtaca acgggggtta
    21541 tcctgcaagc cttaagaatg tggttgatct gttgtacgag gaatggcacg gcaaacccct
    21601 tgccatagcc actgtttcag acggtcattt cgggggctct caggtgatca cctcccttct
    21661 gttttcattg tggaaaatga gggtcagggt cgtgactgcc atgtttccgg ttccgcgggt
    21721 aatggatgcc tttgatgaaa acggaatccc gaaagacaag gcccgtaccg acaaacgggc
    21781 atcaaatttt ctgaaggaac tgtttgccgc catgtgagat ataccggata tccctgtggt
    21841 cctttgctcc ctgcgggatt ccgtctctga agttatagtg atgtgttcag cggctctccg
    21901 gaagagaacc cggaaataaa gcggaaatca ctcagtaggt ccagtatgag ggaccgctgg
    21961 caacgaaatg gaccggaata tcctttatga attccctgag ccattccgcg caatattcca
    22021 ttccggactc ttctgaagta acatgaccca ggaatattac agccttgttc cggttctgac
    22081 tgacagcatc ccttgcatac tcatatgttt cccactgggg aacctctcct gccagcagaa
    22141 catccacggt cggatccctg agaatcctga tgtgcgcact gcttcccggc gcccccgcgg
    22201 caaaggctat tcctgaaagc ttcatgtcag gattgccgat aatatgaaat gcattctccg
    22261 ggaatatcct tttaagataa accagcaatt ccttaaggct tgtgcggggt atgtcaaacc
    22321 tgttgtaacg gttttctgaa acataatccg tccagccaag tttcagggtc atcccgtaat
    22381 ctatcgcatc cggtttcaga ctgtgaatat aatcatgaaa ccgccatacc accagtttat
    22441 tatcatctat aaactttttc ttttccagat aaaccggatc attctgtaac tgctttgtat
    22501 catccaggtg attgtaataa agaggttcgt gggctatgat cagattacag cccagttcct
    22561 ttgctttttt cagtacttcc atagttgcaa acatggtggt gactatgccc ttcacttcag
    22621 ttccgggatc accggcctta atcacatcca cagtgtttgg aatgctcgcg gtccgggtct
    22681 gcctgataat tgtttcaatt atgccggctg cacttttatc cgtggatttc tgccctgcaa
    22741 gcgacaatga aaaaaccacg agaaatgaac tgatgagaag cgattttagt gttttcatat
    22801 cttttagacc attgattgta tttaaatctt cccgtcccgt catcattacc gccctcttac
    22861 aatctcctgt tccggagctt ggggcatcag cggaaaaaaa ttggtacgac ttttgattca
    22921 gacatggtga agagccaatt cttctaacac ttacgacaat gaaaaagtta atattcactc
    22981 cgattctcgc actcctgatt gcagcaggaa ccacagcctc cgcccaggat tttcccgagg
    23041 aatatcttgg cctgccggga gacaatctca acctgtatgc agttatgaaa ctctttcagg
    23101 agtccgaaac ccttgaaggt tttgaaagaa gcttaaatga cgaaaattca aggataaaca
    23161 atcttgatct gaacggtgac aatctgattg attatattac tgttcatgat tatgttgacg
    23221 gtgatgtgca tacgatagtg ctcagagtcg ccctcagccg gaatgaattc caggatgtgg
    23281 ccgttttcac ggttcagaaa ttccgcgacg gctccgtaaa tatccagctg gtaggcgatg
    23341 aagccctgta cggaaagaac tacatcatcg aacccattta caatgaaacg cccaatcccg
    23401 gctatacagg cagggcttcg gctacccgca gagtgaatgt gactgttgta agaaccactc
    23461 cgtatgaagt agcggcctgg ccggtaataa cctatatcta ttcaccaagg tatgtggtat
    23521 ggcgctccgc atggtactgg ggatattatc cttcctactg gcggccctgg agaccctact
    23581 actggcatta ctactatggc tatcactaca actggtatca ctactactat aatcattacc
    23641 actactggcc acattaccgc tatccgagat acaccgccta ctactacacc acggtaaggg
    23701 tgcactcaac cgtcgtggtc aacaatgtca gggacggcta ctacagaacc acctattcac
    23761 ggcctgaaca aaggcgcgag ggcgaagcac tgttcaccag ggttaatcct gaccagacca
    23821 ggagaacggc ggaaaacaca ccggcaccaa gaatgagaag agaagtattc cgtgaaggca
    23881 atgaaaggga tgctgctacc ggcacggcaa gacgccaggc tgaacagaac ggtgaaaggg
    23941 ctgcaacaag atcctctgcc gacaggaata caggaaccga aagaagagcc gcatccactg
    24001 ccggcgacag atccgcagca agaaccgccc cggcacagag cagcagtacc gaaagaaggg
    24061 ctgcatcaac tgccggcgac aggcccgcag ccagaaccgc cccggcacag aacagcagta
    24121 ccgaaagaag ggctgcatca actgccggag acagacctgc ggcaagaacc gccccggcac
    24181 agagcagcag tcaggccaga agtgctcctg ccacggcatc acagagatcc gctgcaaagc
    24241 ctgcaccggc agtcaaatca caaagttccg caggcaccag gcagtcagcg ccggcagcca
    24301 gatccacaca gaccaggccc gccagggaaa gcagacaggc cggtactcag gagagaagtg
    24361 caggcagaaa ggaatcctca gagagttcga atacccggag aaaataatta agcagcagaa
    24421 ctgaagaata aacaaagtac aacatgccgg aagcaaaaac cggaaggaca gccggcagaa
    24481 taaaggaatc acgataaaac gaaacaaaaa atgcgacagg cccgggaccg gaagacaaaa
    24541 agccggatcc cgggtttttt tatttatccg aaaaaaacag gtgaaccgct gtgataaaga
    24601 ttcattaaaa gcaagatcac tcaattatta tccccgcgga tttttgtatt ttccgaaaca
    24661 ttgcatatct ttgtcccctc caggagggga aaattgttct aggggaccga agtcccctat
    24721 tttgttatca ttataagaac atgattgaca aagaaagaat agaaagaatt atcaatgagc
    24781 atattacagg gacggatatg ttccttgtaa gcctgaaggt gagcactgca aacaggatca
    24841 ccgtgcttgc agacacaatg aaaggaatca ccattgatga atgtgcggaa ctgcacaggt
    24901 tcctggaaaa gcaactggac aggaatattg aggattttga attgcaggtt tcatctcccg
    24961 gactggatgc gcctttcagg gtaattcagc agtattacag gaatgaaggc aatatgattg
    25021 aactgaccga tatgaacgga gagaaattta cgggaaaact caggaacgtc actgatggcg
    25081 gatttgagct tgagacagtg attaaggaaa agggcaagag cccttcagtt aaggagcttt
    25141 cctttaatta cgatcagata aaaacggcaa aagcagtatt taaagttaac taacaataag
    25201 agtccttaag gaaatggaaa atgttaattt gatcgacacc ttttctgagt tcaaagagct
    25261 gaagaatata gaccgtccga ccatgatgag cgttcttgaa gacgtgttca ggaatatgct
    25321 tgcaaaaaaa tacggctcag ctgacaattt tgacataatt gtaaacatag acaagggtga
    25381 ttttgagatc tggaggaaca gagtggtggt gaatgacgat gaattatccg atcctgttac
    25441 ccagattcct ctttcagaag cgaaaaagat agatgaggac tatgaggtcg gggaagatgt
    25501 gtcggatgaa gtaaaattcc tcgatttcgg cagaagggca atactgtcct taagacagaa
    25561 cctcaccgca cgtattcttg accttgagaa aaacaatatc tacatgaagt acaaggacag
    25621 aatcggtgag atcgttacag gtgaagtcta ccaggtatgg aagcgggaaa tcctgatcct
    25681 tgatgatgac ggaaatgaac ttattcttcc gaaaagcgag cagataccct ccgatcattt
    25741 ccgcaaggga gattcaatac gggcagtggt catcaaggtt gaaatgagga acaatacacc
    25801 cttcatcatt ttaagccgga cctcccctgt tttccttgaa aggcttttcg aacttgaagt
    25861 gcctgaaata tttgacggac ttataaccat aaagaaaatt gtaagggtgc ccggggaacg
    25921 ggcaaaggtg gccgttgaat catatgacga aagaattgat cccgtgggag cctgcgtagg
    25981 tatgaaaggg tccaggatac atggcatcgt aagggagctg agaaatgaaa acattgatgt
    26041 aatcaattac acaacaaaca atcagctttt catccagaga gcactgagcc cggccaagat
    26101 taccacaata aaactgatag aggagggaaa taccaggaag gccgaaatat ttctcaaacc
    26161 taacgaagtt tcccttgcaa tcggcaaggg tggattgaat atcaagcttg caagtcagct
    26221 tacagggtat gaaatagatg tttacagaga accgggagat gaggatgagg aagatgtaaa
    26281 tctggaagaa ttcagcgacg aaatagaaca gtggattatt gatgagctca agtctatcgg
    26341 atgcgatacc gcaaaaagtg tgctcagcct gtcaataaac gacctggtca agagaactga
    26401 tcttgaggaa gagcagatca gggaggttgt caatatttta agagcagaat tcgagtaatc
    26461 cggacttctg aatcgatgtt ttccggattc caaaataaag agaagggata gttatgacag
    26521 aggaagtaaa ggtaatgcgc ttaagcaaag cagcccgcga gtttaatgta ggaatagcaa
    26581 ccataattga attcctgcac aagaagggct ttgtactcga ttcaaatccc aatacaaaac
    26641 tgcctcccga ggcatgtttg ctgctggaga aagaatacgg tcccgatatc aacctgaaaa
    26701 aggaatccga gaagcttgcc ctgagggatc taaggaataa agcaacactt accctggatg
    26761 atgttaccgg aaaacctcag gtggaagagc cggaaaggga ggaggaaata ctcctgaagg
    26821 atacaacaac caggattgat ataagaacag aaattaaaaa acctgccgtc aagctggtag
    26881 gaaagatcga tcttgaaaaa acagcaaaga ccagaaccga aaaagctgag gaggtaaaaa
    26941 aggaaaaagc tgaaaagaaa gtatccaaac cggcagctaa agatgaaaaa cccgcggaaa
    27001 gcacagcacc agaggaaaag aaactgccca cggttgatct caccatagta gggaaaatag
    27061 acctggaggc agtaagcccc aggcgcgtaa caccggcccc tgaagaagcg gttgaagaaa
    27121 taaaaaaaga ggagaagccc ggggttatca gctccgtgga tgaggaaaaa ccggcagccg
    27181 ctcccgcaga ggcagaaact gaagttgcag ggaaagcaga aatcaccgct gctccggctg
    27241 aggagacaga agagcccatg actgaccagg aagaagtgat tgaactcttc cgtccggaca
    27301 tagcaaagct ttcaggtccg actgtcgtag gcaaaattca gcttcccgcc gaggaaaaga
    27361 agaaaagcgc ccagcagcag ccggtaaaaa ctgatgcaga aagcgaacag agaagaaaga
    27421 aaagaagaaa aaggataacc aaggaaaagg aacaggtcac tgtagccaag gccgacactg
    27481 ccgaaaagaa ggaaaaacag gccaggaagt ctcctaaaag aaggccggtc agggcagagg
    27541 ttgatgaaga ggaagtgcag aagcagatca aggaaactct tgcaagactg acatcaaagg
    27601 gcaaatcaag agggtccagg tacaggaggg agaaaagaga agccataagc cagaaacaca
    27661 gggaatttga agacaggata gaacaggaaa aaaatatcct gaaagtcacg gaattcgttt
    27721 cggtgaatga actcgcgata atgatgaata ttccggttac cgatctcata tcaacctgta
    27781 tgaacctggg acttttcgtt tccattaacc agagactgga tgctgaaacg atggttctcc
    27841 tggcagatga attcggatat aatgttgagt ttgtcagtgc tgatctgctg gcagccgtca
    27901 gggaggaaga ggatgaggaa tatgaacttc agccacgccc gcccatagta acggttatgg
    27961 gacatgttga ccatggcaag acaaagcttc tggactatat aaggcgtacc aacgtaattg
    28021 cgggggaagc aggaggaatt acccagcata ttggtgcata cgcggttaaa cttgatgacc
    28081 agagacagat tacctttctt gacactcccg ggcatgaggc ctttactgcc atgcgtgcaa
    28141 gaggggcaca gataactgat atcgcaatca tcgttgttgc tgccgatgac ggagtgatgc
    28201 cccagactgt ggaggcaatc aatcatgcct cagcagcagg cgttcccata gtctttgcaa
    28261 tcaacaaaat agacaagccc acagcaaatc ccgacaagat aaaggaagct cttgccaata
    28321 tgaattacct tgtcgaggac tggggaggca aatatcagtc acaggaaatc tcggcaaaat
    28381 caggtctgaa tgttgaactg cttctggaca aggtactcct tgaagctgaa atacttgaac
    28441 tgaaggctaa ccccgacaag gcagccatag gcacggtgat agaatcatcc ctcgacaagg
    28501 gaaggggctt cattgcaacg gtacttgtaa aggcaggaac tctcagggtg ggtgatatga
    28561 tgcttgcagg aagccactac gggaacgtaa gggccatgta caatgaaaga ggccacaggg
    28621 tgtctgaagc cggtccttca acgccggcac ttatactcgg gctgaacggc gctccccagg
    28681 caggcgacca gttcaatgtg atggagaccg aaagggaggc aaaggatatt gccaccaaga
    28741 gagcacagct tcagagagaa cagggcctga ggacccagaa gcatatcacc cttgatgaaa
    28801 tagggcgcag gatagccatt ggcaatttcc aggaactgaa tatcattgtc aagggtgacg
    28861 tggacggttc cgtcgaggca ctcggggatt cactgataaa actgtccaca cccgagatac
    28921 agctcaatat cattcacaag gctgtgggcc agatatcaga atcggatgtc ctgctggctg
    28981 cagcttcaaa tgccatcatt gttggattcc aggtaaggcc ctcacatagt gcacgcaagc
    29041 tcgcggaaaa ggaggaaata gacatacgtc tttactcaat catctatgat gcgattgaag
    29101 agatccgttc ggcaatggaa ggaatgcttt cacccgaaat gaaggaggaa attgttgcca
    29161 cccttgaaat acgcgagatc ttcaaggtca caaaagtggg aacagttgcc ggatgctatg
    29221 taaaggaagg aaaaatcagc aggaactcct ccgtcagggt aatccgtgac ggaatagtca
    29281 tctatacggg tgaactgggc tctctcaaac gcttcaagga tgatgcgaag gaagttgccg
    29341 gaggtctgga atgcggtctc aacatccata atttcaacga catcaaggtt ggtgatgtta
    29401 ttgaaggatt taagcagata gagattaaga agacactgta gctgaccctt cagggaccct
    29461 gccggaatcc tgcaggaagc ttacataaat cttgcaggaa gcttacaggg aattacaggg
    29521 attctctgaa agacctgcgg tatgatctta tagaagagcc ttgt
//
LOCUS       DAOSPX010000006        29307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_1752_length_29307_cov_4.982805, whole genome shotgun sequence.
ACCESSION   DAOSPX010000006 DAOSPX010000000
VERSION     DAOSPX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1752_length_29307_cov_4.982805"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..2072
                     /locus_tag="PLZ75_00685"
     CDS             405..2072
                     /locus_tag="PLZ75_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOS70944.1"
                     /translation="MKIGVNNILLELHYGAKVRDAVLKYYSQQGKKVPRPFPRVEDGY
                     GNRVDAEGGLSSGNSLFIMTAGGRRKSLTQLFLLLLLAGLFSACGSSGKVLTGGRTER
                     EAVIFAVNDMHATLDNFPKLAYITDSLRKIYPHMLLVGAGDNQTGNPVNDQYPERGLP
                     MIELMNATGFNLSAVGNHEFDTSPEGFSNLIRKADFDFICANMSAGNPDDFKIKPWKI
                     ITLPNGLKLAFLGLVQINRNGIPDSHPGKLKGFTFRSPFEAARDYLWLKDRSDIFIAL
                     THIGFENDVKLAEIMPPAIDLIIGGHSHTRVEKEQIHNGIMITQADHKLNYGTLIRLS
                     LRSDGSLTRSMQLIRIRKAGNESPAIRAMVDEYNDIPLLKEVIAWASDDFSSKEELGY
                     LMADAQKDIAGADIALVNPGGVRIRSLEKGPVRAKDVYQLDPFGNELIVTRLTGKEIH
                     SLMLAAYPMDDRQPVFSSGIFTVLKPGTGGELADVTLFTGDGTPLDMDKTYTVAMNSY
                     MTQVYQYQHRDPGQSHYITTADALIDYLKKNQNIRSYRNEKRFRIEH"
     assembly_gap    2303..2312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2313..3145
                     /locus_tag="PLZ75_00690"
     CDS             <2313..3145
                     /locus_tag="PLZ75_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOS70945.1"
                     /translation="SEYEDRYAEMVTLFPHAGKEPSYPKDHLLNLRRAKETVSIPVIA
                     SLNAVYDESWVEYAVKIEETGVDALELNFYTVPEKLDNAYGVVEKKHAKIVSAVKAAV
                     KIPVSVKLSPFYTNPLGLIYDIDKAGADGFVLFNRLFQPDIDIETQQHRFPYNLSNLE
                     DKRLPLRFAGLLYGNLRASICTSSGIFNGEDVVKMILAGSDCVQVVSTLYLNQIEVIE
                     KILGDIAKWMDANKYGSIDDFRGKLSRKISGNETLFNRTQYIDFHLNTAEILNKHKIF
                     "
     gene            3425..3979
                     /locus_tag="PLZ75_00695"
     CDS             3425..3979
                     /locus_tag="PLZ75_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOS70946.1"
                     /translation="MTEQIKQIAARIREIREISGVPAETLAQKLGLSEEVYNRYESGN
                     TDIPVGIVFEISEMFNVELSVLLGGNNPKLHVYSIVRRGKGLRLERRQQYRYESLAWN
                     FLNKKAEIFMVTIDPKPEDTLLEFNSHPGQEFNYVIEGSMMSIIDGHEIILNEGDSIF
                     FDSGYNHAMKALNNKQVRFLAMIL"
     gene            4051..5721
                     /locus_tag="PLZ75_00700"
     CDS             4051..5721
                     /locus_tag="PLZ75_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS70947.1"
                     /translation="MLEKYLEQTEFESYENFISGFDIKIPENFNFAYDIVDETAAATP
                     GKTAMVWCDDRGNEAVFTFEQMKYYSSKAAVFFKSLGIRKGDRVMLILKRHYSYWFCT
                     LALNRIGAVTIPATHLLSAKDIVYRNNAADIKMIVSVPDPLVMEHIEEAESQSPTLKY
                     KLLIDRDRKGWLNFSRGMEESPAEFPRPAGDDATENSDTMLLYFTSGTTGMPKMVSHD
                     FTYPLGHIVTAKYWQGVLNGGLHFTIADTGWAKSAWGKIYGQWISGSAVMTYDYDRFV
                     PADILSVLEKYKVTTFCAPPTIYRFLIKEDLTRYDLSSLTNCVVAGEPLNPEVYRFFL
                     EKTGLKLMEGYGQTECTVAIATYPWMEPKPGSMGKPTPGYDIDIVDESGHPCEIGYEG
                     EIVIYTSVRKPVGMFSGYFRDDELTRSVWPDGMYKTGDMAWRDEDGYFWFVGRADDII
                     KSSGYRIGPFEVESALIEHPAVLECAVTAVPDPDRGQIVKASVILSKGFTPGDELAKE
                     LQEHVKKVTAPYKYPRIVEFVTELPKTISGKIRRVQIREENEQAPGDF"
     gene            complement(5718..7070)
                     /locus_tag="PLZ75_00705"
     CDS             complement(5718..7070)
                     /locus_tag="PLZ75_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOS70948.1"
                     /translation="MNPEENAIMKSFGKNESGTRRLLRYPAYLLLTVLVCVLVYTACR
                     EQEEEGDLLEFGMGHLLDNAELQAFGEKLFFDKNLSTPAGQSCASCHGPEAGWTGPDS
                     EINAKGAVYEGAVKGRFSNRKPPSAAYATYSPVFNAMLEEGELLFVGGNFWDGRATGY
                     LLGNAAADQAQQPFLNPVEQNIADDKGVVEKVLNSDYADMYHKIAKDIWNIEDIASCS
                     DVNMQFGIIGLALAAFEHSDRVNAFTSKYDYYLQGKVKLTPQEEKGMDLFRNKGLCAE
                     CHPMDVLEDGTPPLFTDFTYDNLGMPANPDNPWYTMDRSFNSEGREYRDPGLGGFLKN
                     VPHYAMFAEESMGLHKVPTLRNVDKRPSPDFVKSYGHNGYFKSLEEIVHFYNTRDVLP
                     VTDEVDDPRPGINSWPEPEIALNINLDELGDLGLTPEEEDAIVAFMKTLSDGYELPDN
                     "
     gene            complement(7171..7938)
                     /locus_tag="PLZ75_00710"
     CDS             complement(7171..7938)
                     /locus_tag="PLZ75_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOS70949.1"
                     /translation="MKIISWNVNGIRAIVKKSFFTDLELIGPDITCIQETKANDHQVS
                     EALESLNHNHIYTNSAVRPGYSGTAVISSISPLGITKDIGISEHDNEGRVLCLEFEQF
                     FLVNVYVPNSGAELARLGYRQEWDMAFFNYLKDLEKTKPLIVCGDLNVAHREIDLARP
                     RANYNKTAGYMQEEIDGMDRLVNGGFTDTFRHFYPDQTGRYSWWSFRAAARSKNIGWR
                     IDYFLVSPSLLSRVKDAFIHEEIMGSDHCPVGIVLDM"
     gene            complement(8053..8748)
                     /locus_tag="PLZ75_00715"
     CDS             complement(8053..8748)
                     /locus_tag="PLZ75_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOS70950.1"
                     /translation="MKTIFETDREFICDIQAPCFRDLGPEETEFIRSGKTQVLFRKGD
                     NLTKQGAFTSYVLFLITGLAKQYLESNSTKTFNLRIIRPGEFLGLSAVFHGNVFGCSS
                     SALTDCQAFLIEKEAIAKVIKQNGQFGFSLIRKYTEQNNNLLSVIRNLNHKQMNGRLS
                     DTLLYLDALKTEYPEIFTLLSRKDLADFTGISTESTVKLLKTFEKDGLIELQDKDIVI
                     KNHGILSEISKKG"
     gene            8915..9271
                     /locus_tag="PLZ75_00720"
     CDS             8915..9271
                     /locus_tag="PLZ75_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS70951.1"
                     /translation="MLYTNLKHLESAAELERAISENENVMVICGRMGPMCIPVYETAE
                     ELEGEYEHVKFYDMEFDNPEAHVIRSLPEVRGFMGIPFTIYYRDGKVVRATSSIQTEE
                     QIRTILDREFSKTVKA"
     gene            9274..10233
                     /locus_tag="PLZ75_00725"
     CDS             9274..10233
                     /locus_tag="PLZ75_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS70952.1"
                     /translation="MNGDSHYDAIVIGAGPAGLTAGIYLSRARLRTLILNEGTVGGQL
                     VLTHEIANYPGVESINGYRLSGIMKKQALSFGCDIRSNITLTGIELKDAEKRITLSDG
                     SVYTAGAVILSPGGRPRTLGVRGEEELRGRGISYCSTCDGDFFAGREIIVVGGGNSAL
                     EEAVSLTKHASKVTVIHQFGHFQAFEYAIEEARNNPRINFIMESVVTGFNGEEKLESV
                     DIKNLVTGETGNLRTEGAFVFIGYVPNTGFLEGMVPLNRGGEIIVDSDMSVNIEGVYA
                     AGDCTAGKYRQVTTAVGEGTIAALAASAYISELRVKQKQAAEI"
     gene            10235..10654
                     /gene="trxA"
                     /locus_tag="PLZ75_00730"
     CDS             10235..10654
                     /gene="trxA"
                     /locus_tag="PLZ75_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOS70953.1"
                     /translation="MKTALIIIGLLVFVIVLLYIFAKSKIKNTPVVEDHENIIVLSDR
                     NFDHQTKNRVVLVDFWAEWCVPCRMMAPVLNELAEELNGNAFVGKLNVEQYQALAQKF
                     NIRNIPTMILFRNGQEVKRFIGMKTKDFLIREIQKVA"
     gene            10656..11234
                     /locus_tag="PLZ75_00735"
     CDS             10656..11234
                     /locus_tag="PLZ75_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOS70954.1"
                     /translation="MENVSSYSEFSSKMPDSGQSFLLLYREGSVQSACALRNMETALS
                     GSGNPRIFSADVNVAKDIHQVYGIDSVPSLLVFSDGRLVNVIKGCQDSEYYTALTRNR
                     LFRSEQAPDGKSVKNVTVYSTPSCSWCNTLKEWLRKNNIKFRDIDISLDQKAAADLVR
                     RSGQQGVPQTDINGQIVVGFDQPRLKKLLEIQ"
     gene            11258..11545
                     /locus_tag="PLZ75_00740"
     CDS             11258..11545
                     /locus_tag="PLZ75_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOS70955.1"
                     /translation="MKELQPINQLVVLDVTASKDEQRTSSGIIIPDTAKEKPKSAPVV
                     WMGVIENAEIKPGDVVLYKPFAGTEIEFEGKEYLILPYADVLAKVVETEKI"
     gene            11562..11987
                     /locus_tag="PLZ75_00745"
     CDS             11562..11987
                     /locus_tag="PLZ75_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOS70956.1"
                     /translation="MAKKESTSIRWLGNMAFETEINGHKLIIDTGSEAGGGNQGPRPK
                     PLMLTALGGCTAMDVVAILKKMRVDFDSLNVIVEGDLTEEHPKHFSKMHVIYEFTGKD
                     LPVDKLQKAIDLSTEKYCGVSAVYRKAMELTSEIRILEP"
     gene            complement(11990..13237)
                     /locus_tag="PLZ75_00750"
     CDS             complement(11990..13237)
                     /locus_tag="PLZ75_00750"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOS70957.1"
                     /translation="MKKIIVIGGGFAGLQFIKNLKKGLYDIMLFDRLNHHQFQPLFYQ
                     VATSQIEPTSITFPLRKIFQKRKDVSIRYAEVLSVNPGKNSITTTAGDFSYDFLVIAS
                     GGKTNFYGNEQIRKNALSLKSTYEAMKIRNVILENFEKIINAGEEYQDGLFNIVVVGG
                     GPTGVELSGAFAEIKRDILPKDFPGIDFSRLNIFLLEGGKHTLNSMSDMAKRVSEKYL
                     NKLGVIIKKEVFVLDYDGETLKLSNGEVIRSKNVIWAAGITGNIIEGLAPETITLTNR
                     IRVDRMNRAEGYENIYALGDIAYMETPKYPRGHPQVANVAINQGRNLASNFKAMASGR
                     KQTEYEYRDLGSMATVGRNKAIVDLPFIRFHGYFAWYFWMFLHLMLILSVRNKLIIFI
                     NWAWNYVTKNSSLRLILQTDGQK"
     gene            13410..13760
                     /locus_tag="PLZ75_00755"
     CDS             13410..13760
                     /locus_tag="PLZ75_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009434909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOS70958.1"
                     /translation="MLLRRVYDPEEPGEGYRIFIDRLWPRGLSREKATWDEWMKDVSP
                     SNELRKWFGHDPSKWEEFRRRYKKELAGRSGQLEKLRQLEARQGTITLLYSSREERYN
                     NAVALKEFLTQKDG"
     gene            complement(13757..16021)
                     /locus_tag="PLZ75_00760"
     CDS             complement(13757..16021)
                     /locus_tag="PLZ75_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HOS70959.1"
                     /translation="MKTKKLWIVYVLVTVISFAVLIIFGREIYREKPPVPEKVITESG
                     ILLFTSQEIKDGQNIWQSIGGQQLGSIWGHGAYIAPDWSADWLHKEAVFLLDKWSREN
                     FGILFDKLDPERQAMLEVRLQNALRANTYDSKTGILTISDERADAIRSISRHYQGLFM
                     DDPSLNEERAAYAIPANTIKDSGRMDRMNAFFFWTAWACITERPDSELTYTSNWPPDR
                     IVGNTASAGLVLWTGFSVIMLLVGIGLLAYYNARNKSDILDPSLLPQNDPLGGLNPTP
                     SMKATLKYYWVVLGLILMQILFGVVAAHYGVEGKGFYGLSLDNILPYSVSRTWHVQLA
                     LFWIATSWLAAGLYIAPAVSGHEPKYQKLGVNLLFSALLVIVVGSLIGEWMGVMQKLG
                     LVHNFWFGHQGYEYVDLGRFWQIFLFIGFFIWLFLMFRALLPALVKPSENRQLLIMFI
                     IASVAIAGFYGAGLVWGRQTHLSVAEYWRWWVVHLWVEGFFEVFATVIAAFLFVRMGL
                     LNIRFATTGVIFAAIVYMAGGVIGTFHHLYFAGTPTAIIALGASFSALEVVPLVFMGF
                     EAYNNLQLSRATPWVRSYRWPIYFIISVSFWNLVGAGLFGFLINPPIALYYMQGLNTT
                     PVHGHAALFGVFGMLGIGLMLFVLKGLTVKYVWKTGFLKFAFWALNVGLMLMLLLSLL
                     PVGFLQTIASIKHGMWYARSAEFMQDSTMQTLRWLRIVGDVVFAAGSLALAVFVIGLK
                     TGWSIDRSHKLYED"
     gene            complement(16317..16616)
                     /locus_tag="PLZ75_00765"
     CDS             complement(16317..16616)
                     /locus_tag="PLZ75_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70960.1"
                     /translation="MTTGGKGDNYEKETLQHKMTIREDAKQVLIKQLNYINKQFQNET
                     NNNRRFNIHSYARHKSKANPNFFRWLFDDYENIGLYGTNLTSEQKEVYKIWLHNL"
     gene            16984..17655
                     /locus_tag="PLZ75_00770"
     CDS             16984..17655
                     /locus_tag="PLZ75_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70961.1"
                     /translation="MKTHNVRIISTGLITRDVLQIFTEKPEDLVFTPGQAVDISINRE
                     GWKDEKRPFTFTSLPDDDFLQFTIKTYPADRGVTNELLSLRKNDSLILYDVFGAIEYR
                     NEGYFIAGGAGITPFISILRYLKSKNAIGNNKLIYANKTRDDIILKYEFEHLLGKNFV
                     NILSDETAQGYAHGYLSRDFLSKQMADLTKDIYLCGPPSMMEAVEKILSDLGVDDSQI
                     IKEAD"
     gene            17722..17913
                     /locus_tag="PLZ75_00775"
     CDS             17722..17913
                     /locus_tag="PLZ75_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOS70962.1"
                     /translation="MAVTKVWIEEGCTACGVCEDICPEVFKVEDEATVIEGVNYSDYT
                     DEIKEAAESCPVEVIQYSE"
     gene            complement(18041..18730)
                     /gene="pdxH"
                     /locus_tag="PLZ75_00780"
     CDS             complement(18041..18730)
                     /gene="pdxH"
                     /locus_tag="PLZ75_00780"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOS70963.1"
                     /translation="MTQKVNDISFEMKSVHEIIAGSGEEYISGGLNEKDIPADPLELF
                     EKWLEEAVERNPELPNAMHLSTAGADGRPSGRIVLLKAFDKRGFVFYTNLDSRKGREL
                     KENNHAALTFFWDELFRQVRLEGRVFEVPPEESDSYFASRPRESKISALASEQSRVLK
                     NRKDLEDRVKELENSLKGKDVQRPANWGGYYLSPSYMEFWQGRAHRLHDRIVYRRKDN
                     SDRWDIMRLYP"
     gene            complement(18711..20516)
                     /locus_tag="PLZ75_00785"
     CDS             complement(18711..20516)
                     /locus_tag="PLZ75_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70964.1"
                     /translation="MKNTFKQLTRLTALLPVIFISGLKAESQKARTANQLNIKSIELN
                     YPVPAIPFYHFRAEIELPQESIIEVEAAVDGKVLRATDIRRPSDLMNMNRPTIPIRPP
                     SGYNLSHDGTLYKNFILTGWVRWETGKDYSVRISVRLKKNPDPSPGDIWLTATRTVSA
                     PRGSAVFSSDWKNYKALILSETAGISRTGEAVEVLLAFYSDEAQQIERDIRVVAVDPQ
                     TYELTEVPSQVYDRMEYLREDDLDPDEKGKPTRQAELWLPTVTARVAFLADVPAKTSR
                     VYLVFYNNPDAMGKVWTTDLRVQGEAPGLQIENSAISVILHPNSGHLDQITLKSRPDI
                     PLYHRSETNGAMHWNPDIYVPPRPWTHTADWNPPKNVSSVAGSIIARSDVWDDMRKVP
                     EVDASVRYEFFPGVPYFISSTSIRIKETVQALALRNAEMVIKRDLITHAAWYDIVRDS
                     VIIYDVSEIPDFTDLKMEADVPWVMFFNKEKGVGFAGIQLSYSNTGVESPVRVLNPFF
                     YITAGPWVYWARGLSHTFLSSNMQQVIPALKGNVFSERWAYLVFETDKSPDPFTPVTE
                     WRKRLINPLRIDLVEEVDDRVTRIMTEALDDAKSE"
     gene            complement(20701..22377)
                     /locus_tag="PLZ75_00790"
     CDS             complement(20701..22377)
                     /locus_tag="PLZ75_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS70965.1"
                     /translation="MKRREVIKTLSAIPVAGGLMSLDPLMASATQKRSAKEAGLLANA
                     PEGALAPGPQIFQSIGVEPLINCMGTFTAIGGSIPLPEVRKAIEYAALNYVYIDELAM
                     AVGKRLAEITGAEWGMVSSGCAAGMKHVTVACITGGDPEKLVRVPDLTGFPKTEVIIP
                     RYSRNQYDAAIRNAGVTMVEVQTMEELKKAISPRTAMVYLFAAPRDYSEGPLSIDNIA
                     AALKDTDIPILVDAAAEVLTVPNIHLERGATIVAYSGGKAIRGPQGAGLLLGKKDLLF
                     STWQASSPHHGPGRDNKIGREEHIGMLAAVEAWVKTNHPEKEKTWISWLEHISKKVST
                     VKGVSTRIMEPQGVDNRTARLMISWNPDELNITGQEVSKELVETKPRIVLGGGRTNPK
                     DGTTSISISAHMMQPGDEKTVADRIHGILSRQHEKKTVSIEPPAADLSGQWDVSVEFY
                     SGRGEHKLYLGKQEGNDIRGTHKSTFSLLDISGRVEGKNVKLQSYYMENAQSIPYIFD
                     GNIEGDTFSGVLYLGEYRSANFTAKRAERGRRPMMPITVPAHGGKKSDSW"
     gene            complement(22674..23126)
                     /locus_tag="PLZ75_00795"
     CDS             complement(22674..23126)
                     /locus_tag="PLZ75_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOS70966.1"
                     /translation="MKPRLDYSKTAPGALKGMLELEKYVHGSGLERSLYELVKTRASQ
                     INGCAYCIDMHTKDARRAGETEQRLYALSAWRETPFYTQRERAALAWTEALTRISENE
                     ISDSLYKATMEYFSEEEIVALTMAIIAINGWNRLAISFRTTPGSYEPQ"
     gene            complement(23147..23884)
                     /gene="ric"
                     /locus_tag="PLZ75_00800"
     CDS             complement(23147..23884)
                     /gene="ric"
                     /locus_tag="PLZ75_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOS70967.1"
                     /translation="MIISGNTGIGEVVRLNFRTAQLFREYNIDYCCGGEKTISEACSD
                     AGIDADRLISQLEDIAGNSDPDTEYINSLAPDELSDYIVRRHHSYVKKNIPHIRENLD
                     KICDVHGSAHPELFEIRKLFNESASDLAAHMQKEEVMLFPFIREMVESKKQGSRPGPE
                     AFGTVANPVRVMISEHENEGRRFERISEISGSYSPPADACTSYRTAFKQLEDFERDLH
                     RHVHLENNILFPEALRLEKELNESTDK"
     gene            24087..25721
                     /locus_tag="PLZ75_00805"
     CDS             24087..25721
                     /locus_tag="PLZ75_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOS70968.1"
                     /translation="MSRNLRGLSLRKGLTDSMYEQISDAALGADDLREANLISVAREF
                     KTGNAAILSASSFYDFLQPGHQGKKVFVCDGTACLVSGRTEEVMRELLETYQPHEIGT
                     MTCLGHCHTNDAYIAGGEIRYCKCGGGRETPEVYSNAENPVLIVPTGDIRDYYSPLAA
                     LRNNTESALRELGSSGLRGRGGAGFPFHIKVRSALNAATADTKYVVCNGDEGDPGAFS
                     DKWLLEERPHSVLFGMLATGIIIGAGAGVVYIRGEYPLAIKRVKEAIRTLAELGIAAG
                     EENDDSGLFRFHVVQGSGAYICGEETSLLNSIEGLRAEVRTRPPFPAVYGLFGRPTVL
                     SNVETFANVRYILERGGAAWAAMGTAKSPGTKLVSLDGAFNRPGLLEVKMGTPLRNVM
                     DEMGGGTRYPVKAFQIGGPLGGLVPASRIDDLTLDFESFAREGFLLGHAGVVSIPEDF
                     PVIRLVEHLFEFTRKESCGKCFPCRLGSARGHDLVSAAVKDGELIDRQLFDDLLEAME
                     LGSLCALGSGLPLPVKNALQYFGDELKDYFTTIKES"
     gene            25724..28465
                     /gene="fdhF"
                     /locus_tag="PLZ75_00810"
     CDS             25724..28465
                     /gene="fdhF"
                     /locus_tag="PLZ75_00810"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOS70969.1"
                     /translation="MEKTAYIDNKPYAIRDGETILEFVRRNLGREMIPTLCQADNLEN
                     YGSCRICSVDVALEENGPAKVMASCHTPVMPGYYIYPSTDRIRRLRKNILELVLSEYP
                     PERLAPEAGMLPTEFQSVIATAGSPEVRYRRKISKYGKDRTRPYVWSDFTECIKCYRC
                     VRACDELQTERVLWIHGRADRSRIIKDFDRSFIESTCVSCGACVQTCPTNALSDRYSA
                     KTLAADKTVRTTCTYCGVGCNLDVKVIDGAVRAIQAPLDAATNRGHTCLKGRYAFEFY
                     NHPDRLRSPMIRKNGTLTEVSWDEAYEYTAKRFREIREKYGPDALAGISSARCTNEEN
                     YLMQKFFRIVIGTNNIDGCARVCHAPTAMGMQWAFGTGAATNSIDDMYRTGCMLVIGA
                     NPVDAHPVTGARIKSVTESGIPLIVIDPLKIGLARVAKHHLQINPGTNVAVLNLFAYY
                     LLKEGLADEDFIASRTEGWEDFKNYLETLDVDEQERICGVDREEVRAAAIEYGSASEA
                     MEFHGLGVTEHWQATKAITLISNIAMMTGNIGRPGTGVNPLRGQNNVQGAADMGVQPH
                     QGAGYLDVTNPQNIELYTSYYGIEHPSKPGLRIPEMFAAASTGELKALWIMGEDILQT
                     DPNTCHVRSSLQGLEFLVVQEIFMSETARIADVIFPASSFLEKEGTFTNGERRIQKVQ
                     KVVDPLEGTRPDGRIVVEMMNHMAYPQAEYTAAGVLEEIAGIVPFFRGVKWEELGKNG
                     KQWPVLEDGTDTRIMHVGTFKRGKGKFHSWDYESSPELIEHSAEYPYILTTGRLLEHY
                     NSGTMTRRTPNSELVTEDLLYVNPHDAHAKGLVSGDFARIFSARGITTMKVEVTDVVK
                     PGVIYSTFHFPEAGINFLTSGIGDEYTLTPEYKVAAVDFEKSVYGIFARAECRTDLK"
     gene            complement(28486..>29307)
                     /locus_tag="PLZ75_00815"
     CDS             complement(28486..>29307)
                     /locus_tag="PLZ75_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS70970.1"
                     /translation="MDTGDGSLRWSRELTRDFDGVINRYGYNETVLVDGDRLYATPGG
                     VRNNVIALNRFTGDLVWTCPGMGEKAAYCTPAVIRLPSRTLLVTHTESHILGIDIRNG
                     NLLWSYPWANLRLEHQNTPLYKDGLLFCLSGYGMGAVMLRLSPDGTSVSRQWFSQSFD
                     NRMGAAVLINGYLYGSGHNDPSWQCYNWTTGEKMYSSRSLFMGAVISADGLLYCFSEK
                     GEAALVKPDPSGFEIISKMTVRGGSGPHWAHPVIHEGVLYIPRGKALMAYRISQK"
BASE COUNT         7855 a         6657 c         7011 g         7774 t
ORIGIN      
        1 tgagagccgc caggattccc gcaactgctc agataaaaca ataatatgag ggaaaaaacc
       61 aggcctgtat gtttagatct gttcatttca gattgtaatt ccagtttgtc ccctcacagg
      121 agcaggacaa ttaaactttt ttgctttcat ggtttattaa tagcttaagg cagccgtaaa
      181 agtattacca atccgtagat tagtaatacg atttgtaaaa gtataattaa tatttgatat
      241 cctgtccaca aacgctgata tgatgagctt tttaacaaat ttttaattat tcatgaccat
      301 tccggggaaa tacattaact tccttttcag catgcctctt cacagccttt ttttttcaaa
      361 ttaaaaatat ttaaagtttt tttgcagggt aaaatatgat aaagatgaaa atcggagtaa
      421 acaatatttt gcttgaactt cattacggtg cgaaagtaag ggatgcggtt ctgaaatact
      481 attcacagca ggggaagaag gttccccggc cctttccccg ggttgaggac ggctatggaa
      541 acagggtgga tgctgagggt gggctgagca gcgggaacag tctttttatc atgactgcgg
      601 gcggcaggcg gaaatccctg acacagcttt ttctgttgct gcttctggca ggactttttt
      661 ccgcctgcgg ctcttccggg aaggtcctta ccggcggccg gactgaaagg gaggctgtca
      721 ttttcgctgt taatgacatg catgcaaccc tggataattt tccgaaactg gcatatatta
      781 ccgacagtct gcgaaagatc tacccgcaca tgctgctggt aggagccggg gataaccaga
      841 cgggaaatcc ggtgaacgat cagtatcccg aaagaggcct gcccatgatt gagctgatga
      901 atgcaaccgg ctttaacctg agtgcagtgg gaaaccatga attcgatacg agccctgaag
      961 gcttcagcaa tctgatcaga aaggctgatt ttgattttat ctgtgcaaac atgtctgccg
     1021 gtaatccgga tgattttaag atcaaaccct ggaaaatcat cactcttcca aacggattga
     1081 agctggcatt tctcggactt gtacagatta accgcaacgg cattcccgat tcgcatcccg
     1141 ggaagctgaa gggttttact ttccgttccc cttttgaggc tgcccgtgat tacctctggc
     1201 tgaaggacag gagtgatatt tttatagcac tgactcatat cggatttgaa aacgatgtga
     1261 aacttgctga aatcatgccg ccggcgatag acctgataat aggaggccac tcgcatacca
     1321 gggttgaaaa ggaacagatc cataacggga tcatgataac ccaggcagat cacaagctta
     1381 actatggcac tctgatcagg ttatccctga gaagtgatgg cagccttacc aggagcatgc
     1441 agctgataag aatcaggaag gccggaaatg agagtcccgc catacgtgcg atggtggatg
     1501 aatacaatga cattcccctg ctgaaggaag tgattgcatg ggcaagcgat gatttttcat
     1561 cgaaagagga gctgggatac ctgatggctg atgcccagaa ggatattgcc ggagccgata
     1621 ttgcccttgt aaatccggga ggtgttagga tacgcagtct ggaaaagggc cctgtcaggg
     1681 ctaaggatgt gtatcagctt gatccatttg gaaatgaact gatagttacc cgtcttacag
     1741 ggaaagagat ccattccctg atgcttgccg catatcctat ggatgacagg cagccggtct
     1801 tttcttcagg tatattcacg gtacttaagc ccggaaccgg gggtgaactg gcggatgtga
     1861 ctctctttac cggagacggc accccgcttg acatggacaa aacctatacg gttgccatga
     1921 acagctatat gacacaggtt taccagtatc agcacaggga tccgggtcag tcacactata
     1981 ttacaacggc tgatgcgctt attgactatc tgaagaagaa tcagaacata agaagttacc
     2041 ggaatgaaaa aagattccgg atagaacatt gaaaccttga acctgtattg aaccaaactt
     2101 aaacaatatt gttaaataca aaaacactaa acaacatggc tgatctgcaa ataacgtaca
     2161 tgggactgaa actgaaaaac ccggtgattg ccggggcaag caatctttcc ctgaacaagg
     2221 aaaaccttgt aaaaatcgaa aaggccggtg cggcggcagt ggtatacaaa tcactgttcg
     2281 aagaacagtt ccatctcgaa aannnnnnnn nnaatcggaa tatgaagacc gttatgcaga
     2341 aatggtaacc ctgttcccgc atgcaggaaa ggagccttcg tatccgaaag accatctgct
     2401 gaatcttcgg cgggcaaagg aaaccgtttc cataccggtg atagccagcc ttaatgccgt
     2461 atatgacgag tcgtgggtgg aatatgccgt aaaaattgag gaaacgggtg ttgatgcact
     2521 ggaactgaat ttctataccg ttccggaaaa gctggataat gcctacggag tggttgagaa
     2581 gaaacatgca aaaattgtca gtgccgttaa ggcagctgta aaaattcccg tttcagtcaa
     2641 gctgagtccg ttctatacga atcccctggg cctgatatat gatattgaca aagcgggagc
     2701 cgatggattc gtcctgttta acaggctctt ccagcccgat attgatattg aaacccagca
     2761 gcaccgtttt ccctataatc tgagcaacct ggaggacaag aggctgccac tgcgctttgc
     2821 cggtctgctg tatggaaacc tcagggcatc aatatgcaca agctccggaa tattcaacgg
     2881 tgaagacgtg gtaaaaatga ttcttgcagg ctccgactgt gtacaggtgg taagcacact
     2941 ctatctcaac cagattgaag tgattgagaa aattctcggg gatatcgcaa agtggatgga
     3001 tgcaaacaaa tatggatcaa ttgatgattt caggggaaaa ctttcacgga agatctcagg
     3061 gaacgaaacc ctgttcaatc gtacacaata tattgatttt catcttaaca cggcagaaat
     3121 actgaacaag cacaaaatat tctgatcatt atttaaaata ccttttatac agagaatctg
     3181 tcatatagtc gaaatcagag ttgaaggatg gttttgctcc ccgcaatcca tcctttgttt
     3241 cttttgaaat acaggagatc cgctgatatt taatctttta tgccgtttca gggataaggc
     3301 tgaaaagaac ccgaatccgc cggatacgga tttcatcgtg ccggttgcct taatcaggca
     3361 ggctccagga gaattaaaat gagataattt atgtaatatt ggattttcaa aaaaactctt
     3421 ggtcatgaca gaacaaatta aacagattgc tgcgagaatc agggagatcc gtgagatttc
     3481 cggagttccg gctgaaacac ttgcacagaa acttggcctt tcggaagaag tgtacaacag
     3541 gtatgaaagc ggcaatactg atattcccgt aggaatagtt tttgaaatat cggagatgtt
     3601 caatgtggaa ctctcggttc tcctgggagg caacaatccg aaactgcatg tctacagcat
     3661 agtcagaagg ggcaagggct taagactgga acgcaggcag cagtatagat atgagagcct
     3721 tgcctggaat tttctgaaca agaaggctga aatattcatg gttaccatcg atccgaagcc
     3781 ggaggacact ttgcttgagt tcaattccca tccgggtcag gaattcaatt atgtaattga
     3841 gggttccatg atgtcaataa ttgacggaca tgaaatcatc ctcaatgagg gggattccat
     3901 cttctttgat tcaggataca atcatgcaat gaaggctctc aataacaagc aggtaagatt
     3961 cctggcaatg atcctgtaga caggagtgtg aagagaaact gaaaggggaa taacataaaa
     4021 aagcacaaat aaaagtacag gaaagtaatg atactggaga aataccttga gcagacggaa
     4081 tttgaatcat atgagaattt tatatccggc tttgatatta aaattccgga aaatttcaat
     4141 ttcgcctatg atatagttga tgaaacagct gcggcaacac ccgggaaaac tgcaatggta
     4201 tggtgtgatg acaggggaaa tgaagctgtc ttcacttttg aacagatgaa gtattacagc
     4261 tccaaggctg cggttttttt taaatcgctt ggcatccgga aaggcgaccg tgtaatgctt
     4321 atactgaaaa ggcactacag ctactggttc tgtaccctgg ctctgaacag gataggtgcg
     4381 gtaaccatac cggcgaccca tctgctttcc gcaaaagaca ttgtttacag gaataatgcc
     4441 gctgatataa aaatgatagt aagtgttcct gatcctcttg tcatggagca tattgaggaa
     4501 gcagaaagcc agtcgcccac tttgaaatat aagctgctga ttgaccggga caggaaagga
     4561 tggctgaatt tttccagggg catggaagag agccctgcgg aatttcccag accggccggg
     4621 gatgatgcaa cagaaaacag cgataccatg cttctttact ttacttcggg gacaacagga
     4681 atgcccaaga tggtaagcca tgatttcacc taccccctcg gacacatagt aacggcaaaa
     4741 tactggcagg gagtactgaa cggaggtctt cattttacca ttgcagatac cggctgggcc
     4801 aaatcggcat ggggcaagat ttatggccag tggatatcgg gcagtgctgt catgacctac
     4861 gattacgaca ggtttgtacc cgcagatata ctgtcagtac ttgagaaata taaggttacc
     4921 acattctgtg ctcctcctac aatatacagg ttccttatca aggaagacct gacccgttat
     4981 gatctgagca gccttacaaa ttgtgttgtt gctggagaac ccctgaatcc cgaggtatac
     5041 aggtttttcc tggaaaaaac cggactgaag ctgatggaag gatacgggca gaccgaatgt
     5101 accgtggcaa tagcaaccta tccgtggatg gaacccaagc cggggtcaat ggggaaacca
     5161 acacccggat atgatattga tattgtggat gaatcgggcc atccatgcga gatcggctat
     5221 gaaggggaga ttgtaattta cacttcagtg agaaagcctg tgggtatgtt cagtggctat
     5281 ttccgcgatg atgagctgac aagaagtgta tggcccgacg gcatgtacaa gaccggcgac
     5341 atggcctggc gtgatgagga cggttatttc tggtttgtgg gaagggccga tgatatcatt
     5401 aaaagctccg gttaccgcat tggtccgttt gaagttgaaa gtgcccttat tgaacatcct
     5461 gcagtgcttg aatgtgccgt tacagcggtt cctgatcccg acaggggaca gatagtgaag
     5521 gcctcggtta ttctgtcgaa aggcttcact ccgggtgatg aactggcaaa ggagctgcag
     5581 gaacatgtta aaaaagtaac tgcaccttat aaatatccga gaatagtaga gtttgttacc
     5641 gagctgccca aaaccataag cggcaagata cggagagtgc agataaggga ggaaaacgaa
     5701 caggcacccg gggattttta attgtccggt aattcgtatc cgtccgaaag ggtcttcatg
     5761 aaggccacta tggcatcctc ctcttcaggt gtcagtccga gatcgccaag ctcatcaaga
     5821 ttaatgttca gggctatttc cggttccggc caggaattga ttccgggtct gggatcatca
     5881 acctcatccg tcaccggaag aacgtcacgt gtattgtaaa aatgaacaat ctcctccagg
     5941 ctcttgaaat aaccattatg accgtaggat ttaacaaaat ccggagaagg gcgcttatcc
     6001 acatttctca gtgtcggcac cttgtgcagg cccatactct cttcagcaaa catcgcataa
     6061 tgaggaacat tcttcaggaa acctcccagt ccgggatccc tgtattccct gccttcgctg
     6121 ttgaaggatc tgtccattgt ataccaggga ttatcggggt tggccggcat cccgaggttg
     6181 tcatatgtga aatccgtaaa aagaggaggc gtcccgtcct caagaacatc catgggatga
     6241 cattcggcac aaagtccctt gttcctgaaa agatccattc ccttttcctc ctggggtgtc
     6301 agcttcacct tgccctgaag gtaatagtca tatttggagg tgaaggcatt caccctgtct
     6361 gaatgttcaa aggcggccag agccaggccg ataatgccga actgcatgtt aacatcactg
     6421 catgaggcaa tgtcctcaat attccagata tcctttgcta ttttatgata catatccgca
     6481 taatcggagt tcagcacctt ttccacaact cctttatcat ccgcaatgtt ctgttccacg
     6541 ggattgagaa atggctgctg tgcctgatcg gctgcagcat ttccaagcag atagcccgtt
     6601 gcccttccgt cccagaaatt accgccgaca aaaagcagtt ccccttcctc aagcatcgcg
     6661 ttgaaaacgg gactgtaggt ggcataggcg gcgctcggtg gtttcctgtt gctgaacctt
     6721 ccctttacgg cgccctcata tacggcacct ttggcgttta tctcactgtc aggccctgtc
     6781 catcctgctt cgggcccgtg acatgaggca caggactggc cggcaggagt tgataagttc
     6841 ttgtcaaaga aaagcttctc tccaaaagcc tgcagctctg cattgtccag cagatgtccc
     6901 atgccaaact caagcaggtc cccttcctct tcctgttcac ggcaggcggt gtatacaaga
     6961 acacaaacca ggacagtcag cagcaggtaa gccggatagc gcagcagtct gcgtgttccg
     7021 gattcattct tcccgaatga tttcattatt gcattttctt caggattcat gattttatac
     7081 ttttaattca attattttag gtcagtatca aaaataataa atttatagcc ttcatataac
     7141 aggattttgc ataaataaca aagtccgccg ttacatgtca aggactattc ctaccggaca
     7201 atggtcggat cccattattt cctcgtggat aaatgcatcc ttaacccttg aaaggagaga
     7261 tgggcttaca aggaaatagt cgattctcca gccgatgttc tttgatcttg cagcagccct
     7321 gaagctccac caggaatatc ttcccgtctg atcggggtaa aaatggcgga aggtatcggt
     7381 gaatcccccg tttacaagtc tgtccattcc gtcaatttcc tcctgcatgt atccggctgt
     7441 cttgttgtaa ttggccctgg gccgtgccag gtcaatctcc ctgtgggcaa cattaagatc
     7501 tccgcatacg atcaggggct tcgttttttc aagatctttc aggtagttaa aaaaggccat
     7561 gtcccattcc tgcctgtaac cgagccttgc cagctcggcc ccggaattgg gcacatacac
     7621 gtttaccagg aagaattgtt caaattcaag gcacagaacc cggccttcgt tatcgtgctc
     7681 actgatgccg atatcttttg taatgccaag cggactgata cttgaaataa ccgccgttcc
     7741 ggaatatccc ggcctgactg ccgaattcgt gtaaatatga ttgtgattaa gggactcaag
     7801 tgcttcactg acctgatgat catttgcctt tgtctcctga atgcaggtaa tgtcagggcc
     7861 aataagttca agatcagtaa agaatgattt tttaactatt gcccggatgc cgtttacgtt
     7921 ccaggatatt attttcatgg ttagaatttt gttttgcgta aaaatagcaa tactgatggg
     7981 aatagcagaa gaaaaagcag gctatatgct tttttccggc tattcaggtt gtaaagccgt
     8041 aagaattgaa tatcagcctt ttttgcttat ttcggaaagg atgccgtgat tttttattac
     8101 tatatcctta tcctgaagtt ctataagacc gtctttttcg aatgtcttca gcagtttcac
     8161 cgtactctcc gtggaaattc ccgtaaaatc agccaggtct tttctggaca ggagcgtgaa
     8221 tatttccgga tactcagtct tcagggcatc cagatataat aatgtatcag acagacggcc
     8281 gttcatctgt ttatgattca ggttccgtat cacggaaagc agattgttgt tctgctcggt
     8341 gtatttcctg atgaggctga agccgaactg tccgttttgc tttattacct ttgcaatagc
     8401 ttccttttct ataaggaatg cctggcagtc cgtaagcgcg gaagacgaac agccaaacac
     8461 atttccatga aatacggctg ataacccgag aaactccccg ggcctgatga ttctcaggtt
     8521 gaaggttttt gtactgttgc tttcaagata ctgctttgcc aggccggtaa tcaggaagag
     8581 tacataggat gtgaatgctc cctgcttggt gaggttgtct cccttcctga acagcacctg
     8641 tgtttttccc gatcttatga attcggtttc ttcaggtccg agatccctga aacagggtgc
     8701 ctgtatatca caaataaatt cacggtctgt ttcaaaaatt gttttcatat tccttttccc
     8761 gcatgagggg gtagaatccg gcataaagtt aattcaaatc aattaaaaag ttgtcctttt
     8821 tcaagcggag gattgccctg ccgggcactc aggtcctttc ccggcagcaa acctttgatg
     8881 tatttttgcc gcgtaatttt aaaattgaaa attcatgctt tacacaaatc tgaaacatct
     8941 ggaatcggcc gccgaactgg aaagagcaat cagcgaaaac gaaaatgtaa tggtaatatg
     9001 cggacgtatg ggtccgatgt gtattccggt ttatgaaact gccgaggaac ttgagggaga
     9061 atatgaacat gtgaaattct atgatatgga attcgataac cccgaggctc atgtcataag
     9121 atcgcttccc gaagtcaggg gattcatggg aatacctttt acaatttact acagggacgg
     9181 aaaggttgta agggcaactt caagcattca gaccgaagaa cagataagga caattcttga
     9241 cagggagttt tcaaaaactg taaaggcata aacatgaacg gagacagtca ttatgacgcg
     9301 atagtcatag gagcaggtcc cgccggcctg accgccggca tttatctttc acgggcccgg
     9361 ctcaggacat tgattctgaa tgaaggcacg gtcgggggac aactggtttt gactcatgag
     9421 atagccaact atccgggtgt ggaaagcatt aacggctacc gtctgtcggg aataatgaaa
     9481 aaacaggcat tgagctttgg ctgtgatata aggtcaaaca tcacacttac aggtattgag
     9541 ctgaaggatg ctgaaaagcg gataactctt tcagacggca gcgtctacac tgccggtgcc
     9601 gtgatcctca gtcccggagg aagacccaga acactcgggg taagggggga agaagaactc
     9661 agaggcagag gaatatccta ctgttcaact tgtgacggtg atttttttgc cggcagggaa
     9721 ataattgttg tcggaggagg gaattcggcc cttgaggaag ccgtatcgct tacaaaacat
     9781 gcaagtaagg tgaccgtgat tcaccagttc ggtcattttc aggcttttga atatgcaatt
     9841 gaggaagcac gcaataaccc ccggataaac tttattatgg aatctgtggt aacgggattc
     9901 aatggagaag agaagcttga aagcgttgat ataaagaatc tggttacggg agaaaccggg
     9961 aatctcagaa cagagggagc atttgtgttt ataggctatg tgcccaatac cggattcctt
    10021 gaaggtatgg taccactgaa ccggggggga gagataattg ttgacagtga tatgtcggta
    10081 aatattgaag gagtatatgc tgcaggtgac tgcacggcag ggaaataccg gcaggtgaca
    10141 accgcagtgg gggaagggac catagctgca cttgcagctt cggcctatat cagcgaatta
    10201 agggtgaaac aaaagcaggc ggctgaaatt taatatgaaa acagcattaa tcattatagg
    10261 gctattggtt tttgtaattg tattgttgta tatttttgca aaatcaaaaa taaagaatac
    10321 cccggttgta gaggatcatg aaaatataat cgtcctcagc gacaggaatt tcgatcatca
    10381 gacaaaaaac cgggttgttc ttgttgattt ctgggcagag tggtgtgtcc cgtgcaggat
    10441 gatggcacct gtccttaacg agcttgcaga agaactgaac ggcaatgcct ttgtggggaa
    10501 gctgaatgtg gagcaatacc aggctctggc acaaaaattc aatatcagga atattcccac
    10561 aatgatattg ttcaggaacg ggcaggaggt aaaacgtttt atcggaatga aaacaaagga
    10621 tttccttatc agggagattc aaaaagttgc ctgagatgga aaatgtatca tcatattctg
    10681 aattcagttc aaaaatgccg gacagcggac aatccttcct gttgttgtac agggagggaa
    10741 gtgtacagag tgcctgtgcg ctgaggaata tggaaactgc cctgtccgga agcggaaacc
    10801 ccaggatatt ttcagctgat gtaaatgttg caaaggatat acatcaggtt tatggtattg
    10861 acagtgtgcc ttcgctgctt gtcttttctg acggcaggct ggtaaatgtg atcaagggct
    10921 gccaggacag tgaatattat actgcactga cccggaacag gctgttccgt tcagaacagg
    10981 ctccggacgg gaaatcagta aaaaatgtga cagtttattc cactccttcc tgttcatggt
    11041 gcaataccct gaaggaatgg ctcaggaaga acaatataaa attcagggat attgatatct
    11101 ccctcgatca gaaagcagcc gcggatcttg tccgcaggtc tgggcagcag ggcgttccgc
    11161 agactgatat caacggacag atcgttgtag gtttcgatca gcctcgtctt aagaaacttc
    11221 ttgaaataca ataggtttaa ctttaaaata tttaaacatg aaagaattac agccaattaa
    11281 tcagcttgtt gtgcttgacg ttaccgcatc aaaagatgaa cagagaacct catccggaat
    11341 tataattccc gacacggcga aggaaaaacc aaaatcggct cccgttgtct ggatgggagt
    11401 gattgagaat gccgagataa aacccggtga tgttgtattg tataaacctt ttgcaggaac
    11461 ggaaatagaa tttgagggaa aggaatatct tattctgccc tatgcggatg tcctggcaaa
    11521 ggttgttgaa acagaaaaaa tttaattact gagctggtta aatggcaaaa aaagaatcca
    11581 cttccatccg gtggctcggg aacatggctt ttgaaacgga aatcaatggt cacaaactga
    11641 ttattgatac aggatctgaa gcaggcgggg gaaaccaggg tccgagaccc aaaccgctga
    11701 tgcttactgc cctgggtggc tgcaccgcca tggatgtggt ggcgatcctg aaaaaaatgc
    11761 gtgttgattt tgattcactg aatgttattg tggaaggtga cctgactgaa gaacatccga
    11821 aacacttcag caaaatgcat gtcatttatg aatttacagg taaagatctt ccggtggata
    11881 aacttcagaa agcaatagat ctgtcaacgg aaaaatattg tggtgtaagc gcggtgtaca
    11941 gaaaagccat ggaactgact tcagaaatca ggattctgga gccctgagct tatttctgac
    12001 cgtcggtctg caggatgagc cgcagggaac tgtttttcgt gacgtagttc catgcccagt
    12061 tgatgaatat gatcagcttg ttcctgacac tcaggataag catcagatga aggaacatcc
    12121 agaagtacca tgcaaaatat ccgtggaacc tgataaaggg caggtccacg atggctttgt
    12181 tccttcccac tgtagccatt gatccaagat ccctgtactc atattcggtc tgcttcctgc
    12241 cggatgccat cgccttaaag ttacttgcaa gattccttcc ctggttgatt gcaacattgg
    12301 caacctgggg atggcccctg ggatattttg gtgtttccat ataggcaata tcgccgaggg
    12361 cataaatatt ttcatatcct tcagccctgt tcattctgtc aacccttata cggtttgtaa
    12421 gggttattgt ctcaggagcc agaccctcta ttatattacc ggttattccg gcagcccata
    12481 ttacattttt gcttctgata acttccccgt tgcttaattt cagtgtttca ccgtcataat
    12541 ccagtacaaa aacttccttt ttgattatca ctcccagctt attcagatat ttctccgata
    12601 ctcttttcgc catgtcactc atgctgttca gggtatgctt tcctccttcc agaaggaaaa
    12661 tattcagtct tgaaaaatca attccgggaa aatccttcgg gagaatgtct ctctttattt
    12721 cggcaaacgc cccggacagt tcaacgcccg taggtccgcc tcccacaaca acgatattga
    12781 agaggccgtc ctggtattcc tcaccggcat tgatgatttt ctcaaagttc tccagaatga
    12841 cattgcgtat cttcattgcc tcataagtcg atttaagact tagtgcattc ttccttatct
    12901 gctcatttcc gtagaaattt gtttttccgc cgctggcaat taccagaaaa tcataactga
    12961 agtcacctgc agtggtcgta atggaatttt ttccggggtt gacggatagt acttccgcgt
    13021 agcggatgct gacatccttt cttttctgga atattttcct cagcggaaaa gtgatgcttg
    13081 tgggttcaat ctgcgatgtg gcaacctggt agaacagtgg ttgaaactgg tgatggttca
    13141 ggcggtcgaa cagcatgatg tcatacaatc ccttcttcag gttttttata aactgcagac
    13201 ctgcaaatcc gcctccgatg acaattattt ttttcatatc aaatcaggaa aaagaaaatt
    13261 attcaaaaac cgtatggtga ttcatccggc aggttagttt tcgggaggac atcctttcag
    13321 tatgtcacgg atcattgttt tgagtcactc gttaaacaaa ttattgctgt gattgttttg
    13381 cattatggca ttaaaaaata aatgtgatga ttttactcag aagggtttat gatccggagg
    13441 aaccgggtga aggttacagg attttcatcg acaggctgtg gcccaggggg ctgtccaggg
    13501 agaaggcaac atgggatgaa tggatgaagg atgtgtcacc cagtaatgag ctgcgaaagt
    13561 ggttcggtca cgatccttca aaatgggagg aattcagaag gcgctataaa aaggaactgg
    13621 ccggcaggtc cggccagctt gaaaagctca ggcagctgga agcccggcag ggcaccatta
    13681 cacttttgta ttcctccagg gaggaaaggt ataataatgc cgttgcctta aaggagttcc
    13741 ttactcagaa ggatggttaa tcctcatata gcttatggct cctgtctatt gaccagccgg
    13801 ttttcagacc tattacaaaa acagccaggg ccagtgatcc ggcggcaaaa acgacatcac
    13861 ccacaatcct cagccagcgc agtgtctgca ttgtggaatc ctgcatgaat tctgccgaac
    13921 gggcatacca catcccatgc tttatgcttg caattgtttg caggaatcct accggaagaa
    13981 gactcagcag aagcatgagc atcaatccta cattcaaagc ccagaaggcg aatttcagaa
    14041 atccggtttt ccatacatat ttaacagtca gtcccttgag gacaaatagc attaatccta
    14101 ttcccagcat gccaaaaaca ccaaacaggg cggcatgccc gtgaaccggt gttgtattca
    14161 gtccctgcat ataatacagg gctatcggag gattgatgag gaagccgaac agtccggccc
    14221 cgacaagatt ccagaagctg actgatataa tgaaataaat gggccacctg taggatctga
    14281 cccagggtgt tgccctgctc agctgcaggt tattgtaggc ttcaaaaccc atgaacacaa
    14341 gcggaacaac ttcaagagca ctgaaggatg cgccaagtgc aataatggca gtgggtgttc
    14401 ctgcaaaata gagatggtgg aaagtgccga taacaccgcc agccatatat acgatggctg
    14461 caaaaataac tccggtggtg gcaaatctga tattcagcag acccattctt acaaacagga
    14521 aagcagctat aacggtggca aaaacctcaa agaatccttc cacccagaga tgaaccaccc
    14581 accatctcca gtattccgca acagacaggt gagtctgcct tccccataca agtcctgcgc
    14641 cgtagaaccc ggcaattgca actgaggcta taataaacat tatcagaagc tgccggtttt
    14701 ccgatggttt tacaagggca ggcagcagtg cacggaacat caggaaaagc cagatgaaaa
    14761 agccgataaa caggaatatc tgccagaatc ttcccaggtc cacatattca tagccctgat
    14821 gtccgaacca gaaattatga acgagtccga gtttctgcat aacccccatc cattcaccga
    14881 taagtgaacc gacaactatg accaggaggg ccgaaaaaag caggttcact cccagtttct
    14941 gatattttgg ttcatggccc gatacggccg gagctatata gaggccggcg gcaagccacg
    15001 aagttgcaat ccagaacagg gccagctgaa catgccaggt acgtgatacg gaatatggca
    15061 ggatattatc caggcttagt ccgtagaacc ccttgccttc caccccgtaa tgtgcggcta
    15121 ccactccgaa caggatctgc attaggataa gacccagaac aacccagtaa tatttcaggg
    15181 ttgccttcat tgacggagtg gggtttaatc cgccgagcgg atcattctgc ggcaaaagtg
    15241 aaggatcaag aatgtcggat ttgtttcttg cattgtaata agccagcagt ccgataccca
    15301 caagcagcat tatcacgctg aatccggtcc acagtaccag tcctgccgat gcagtattcc
    15361 ccacaatcct gtcggggggc cagttgcttg tataggtcag ttcggaatcc ggcctttccg
    15421 taatacaggc ccaggcagtc cagaagaaaa aggcattcat cctgtccatc cggccggaat
    15481 ccttaattgt attggctggg attgcatagg cagccctctc ctcattcagt gacggatcgt
    15541 ccataaacag tccctggtaa tgcctgctga tgcttctgat ggcatcggcc ctttcgtcgg
    15601 atattgtaag aattcccgtt ttactgtcat atgtgttggc acgtaaggca ttctgcagtc
    15661 tcacttccag catggcctgc cgttccgggt caagtttatc gaacaggatt ccgaaatttt
    15721 cccgcgacca tttgtcaagc aggaaaacag cttccttgtg aagccagtca gccgaccagt
    15781 ccggggcaat ataggcgcca tgcccccaga tcgatccgag ttgctgccct ccgatggact
    15841 gccagatatt ctgaccgtct ttgatctcct gggaggtaaa aagcaaaata ccactttccg
    15901 taatcacctt ttcgggaaca ggcggtttct ccctgtagat ctcccgtccg aaaattatca
    15961 gaactgcaaa actgattaca gttacaagaa cataaactat ccagagtttt tttgttttca
    16021 tgagggtcaa tgaatgagtt ttaggatacg tagtataaat caagcgccta aaggtaacaa
    16081 ggtattcctt attaccaatc ttttacaata tgattattac aattatcggg aaaaaacagg
    16141 aaataaaggc tatgttcagg agatggaatg aacggactat agatgtttgt ctgtcagtaa
    16201 gataaatgtt ttaaacagca gccggacagc aaatccggaa actgcggtta ttcctgcctc
    16261 ttctttaccc ggcggctgca tggggtaccg gaagtgcaaa cattgcaggt catggattac
    16321 agattgtgta accatatctt gtatacttcc ttctgttcgc tggtcagatt tgtcccgtaa
    16381 agaccgatgt tctcataatc atcaaacaac catctgaaga aattgggatt tgctttggat
    16441 ttatgcctgg cataactgtg aatattgaat ctcctgttgt tgtttgtttc gttctgaaat
    16501 tgcttgttga tataattcaa ctgttttatc agtacctgct ttgcgtcttc cctgattgtc
    16561 attttgtgct gcagtgtttc tttttcgtaa ttatctccct ttccgcctgt agtcattgta
    16621 tctgataata gtaaactgta ttttatcttc accaaaaagg gggactataa agcccccctt
    16681 caggtgttct ttataatccg gctaacaaat agtaataatt gccaaaaatt tgtacagctc
    16741 tttctgtgct attgatttca ttaagtcttt ttattctgat cctacaatat ataattcctt
    16801 tactgataaa gcaaatatcc agagcatttt agaatgattc taatttgttt ccgccctgtt
    16861 ccagcattcg tttttaacat ctaccccact tgctcctcag gaaacttttc ctgaagaaat
    16921 ttgtttaaat ggcagagttt attttatgca ttccggttaa ttggttttat aaaaaaataa
    16981 aaaatgaaaa cacataatgt aagaatcata tcaacaggac tcattacccg tgatgtgctt
    17041 cagattttta ccgaaaagcc tgaggatctg gttttcacgc cggggcaggc cgttgacatt
    17101 tcgataaaca gggaaggatg gaaggatgaa aagaggccct ttacctttac aagccttccg
    17161 gacgacgatt ttcttcagtt tacaattaaa acctatcccg cagacagggg tgtgacaaac
    17221 gaacttctca gcctgagaaa aaatgacagc ctgattttat acgatgtttt cggggcaatt
    17281 gaatacagga acgagggata tttcattgcc ggaggtgccg gtataacccc gtttatatcc
    17341 attcttagat atctgaaatc gaaaaatgcc atcgggaaca acaagcttat ctatgcaaac
    17401 aagaccaggg atgatattat tctgaagtat gaatttgagc atcttctggg gaaaaatttt
    17461 gtgaacattc tttcggacga aacagcacag ggatatgcac atggatattt atccagggat
    17521 tttctcagta aacagatggc agatctcaca aaggatattt atctttgcgg acctccttcc
    17581 atgatggagg ctgttgaaaa aatactgtcg gatctcggtg ttgatgacag tcagattata
    17641 aaggaagcag attaaatggg ctttcctgcc ggcgacaggc ggattattga aagccccgtt
    17701 caataattaa tatattaggc tatggctgtt acaaaggtat ggatagaaga aggctgcacc
    17761 gcatgcggtg tatgcgaaga tatttgtcct gaagtattta aagtggaaga cgaagcaacg
    17821 gttattgaag gtgtgaatta ttccgattac accgatgaaa taaaggaagc agcggaaagc
    17881 tgtcctgttg aggtgataca atattcggaa taagacaggt acaaattgat ttgacgaagc
    17941 attcaggcat attgcttatg aaggcagatg ctgccgggct ccggacagac aagggttcag
    18001 ccctgtctga ggatgcatga tactaccttt tgaaactttt ctagggatat aatctcatga
    18061 tatcccatct gtcactattg tcttttctcc tgtagacaat cctgtcatgc aggcggtgag
    18121 cccttccctg ccagaattcc atgtatgaag gagacaggta atagccgccc cagttggcag
    18181 gtctttgaac atccttaccc ttcaggctgt tttccagctc ctttaccctg tcttcaagat
    18241 ccttccggtt tttcagaacc ctgctctgct ccgaagccag tgcactgatt ttactttcac
    18301 gcggccggct tgcaaaatat gaatccgatt cttcgggagg aacttcaaaa accctgcctt
    18361 ccagtcgcac ctgacggaat aactcatccc agaaaaatgt aagtgctgca tggttgtttt
    18421 ccttcagctc ccttcccttt ctgctgtcaa gattggtgta aaagacaaat cctcttttat
    18481 caaaagcctt gagcaatact atccttcccg atggcctgcc gtctgctccc gctgttgaaa
    18541 gatgcattgc attgggcagt tcaggattcc tttccactgc ttcttccagc catttctcaa
    18601 aaagttcaag aggatcggca ggaatatctt tttcattcag acctccggaa atgtattcct
    18661 ctccggaacc ggcaattatt tcatgtacgg atttcatttc aaaacttata tcattcactt
    18721 tttgcgtcat ccagtgcttc tgtcataatt ctggttaccc tgtcatcgac ctcttccacg
    18781 agatcaatcc tgagagggtt gatcagcctt ttccgccatt cggtgaccgg agtaaaagga
    18841 tcagggctct tgtcggtttc aaaaaccagg taagcccatc tttcactgaa tacatttcct
    18901 ttcagcgccg ggataacctg ctgcatattg gatgacagga aggtgtgtga caggcctctt
    18961 gcccagtata cccacggacc tgcagtgata tagaaaaagg gattgagcac tcttaccggt
    19021 gattcaactc cggtatttga atagctgagc tgaatgcctg caaatccgac acctttttcc
    19081 ttgttgaaaa acataaccca gggtacgtcg gcttccattt tcagatcggt aaaatcaggt
    19141 atttccgata catcataaat aataactgaa tcccttacta tgtcgtacca ggctgcatgt
    19201 gttatcagat cccttttaat gaccatctca gcatttctga gagccagggc ctggacggtt
    19261 tcctttattc tgatactggt ggagcttatg aaatagggca ctccgggaaa aaactcatag
    19321 cggacagagg catcaacttc aggaactttt ctcatatcat cccacacatc gctgcgggca
    19381 atgatggatc cggcaacgct gctgacattc ttcgggggat tccagtctgc tgtatgggtc
    19441 catggccttg gcggcacgta aatatccgga ttccagtgca tggcgccgtt ggtttcagac
    19501 cggtgataca gcgggatgtc cggcctgctc ttaagggtga tctgatcgag atggccggaa
    19561 ttcggatgaa ggatgaccga gattgcactg ttttctatct gaagtcccgg cgcctcgccc
    19621 tgtacacgaa gatcggtagt ccagaccttt cccatggcat cgggattgtt atagaacaca
    19681 aggtacaccc tgcttgtctt tgcaggaaca tcagcaagga atgccaccct tgccgttact
    19741 gtaggcagcc agagctctgc ctgccgtgtg ggtttcccct tttcatccgg atcaaggtca
    19801 tcttccctga ggtactccat ccggtcataa acctgcgacg gaacctcagt cagctcataa
    19861 gtttgcgggt caacggcaac aacccgtatg tccctttcaa tctgctgtgc ttcatcggaa
    19921 taaaatgcaa gcaacacctc aaccgcttcc cctgtacggc ttattcctgc tgtttcactc
    19981 aggataagag ctttatagtt tttccagtca gaactgaata cggcacttcc ccggggagca
    20041 ctcacggtcc tggtggctgt cagccatata tcccccggag agggatccgg attcttcttc
    20101 agccttaccg agattctgac ggaataatcc ttccccgttt cccatcttac ccagccggta
    20161 agaatgaaat ttttatacag tgttccgtca tgtgagagat tgtatcccga cggcggcctt
    20221 atcggaatgg tgggcctgtt catattcatg agatcggaag gcctccttat gtcggtggct
    20281 ctcaaaacct tgccgtccac agctgcttca acctcaatta tggattcctg gggcagttca
    20341 atctcggccc tgaaatgata gaacggaatg gccggcacag gataattaag ctctattgat
    20401 ttgatgttga gctggttggc cgtcctggcc ttctgagact cagctttaag gccagaaata
    20461 aatattactg gcagcaatgc cgtaagcctt gtcagctgtt taaatgtatt tttcattttt
    20521 attcgggtta tgctctggta ctgtttgcag gataatttgc cgggattatt cgtgactgcg
    20581 ggccgttttc aaggccttcc ctgttttccg gccggaaggc ctggcagctg agagttaatt
    20641 caatggtcct cccgggaggg agaaccattg aacggttaat gatgaattat tttatgctca
    20701 tcaccatgaa tcgctttttt taccgccatg ggcgggtacc gtgatgggca tcatgggacg
    20761 tctgcccctt tccgcccttt tcgcggtgaa atttgcggaa cggtattcac ccaggtacag
    20821 cactcctgaa aatgtatctc cctcaatgtt tccgtcaaag atatagggta ttgactgggc
    20881 gttttccatg tagtaactct gcagttttac atttttaccc tcaacccttc cggaaatatc
    20941 aagcaatgag aaggtgcttt tgtgagttcc cctgatatca tttccttcct gtttgccgag
    21001 atagagcttg tgttcccccc tgccggaata gaactcgaca cttacatccc actgtccgga
    21061 aagatctgct gccggtggtt cgattgaaac agtctttttc tcatgctgtc tggacagtat
    21121 gccgtgaatc ctgtcggcaa cggttttctc atcccccggc tgcatcatgt gtgcgcttat
    21181 ggatattgat gttgtgccgt ccttcgggtt tgtgcggccg ccacccagta caatcctcgg
    21241 ttttgtttca acaagttcct ttgatacttc ctgaccggta atgttcagtt catcgggatt
    21301 ccagctgatc atcagacggg cagtgcggtt atccactccc tgcggttcca ttatcctcgt
    21361 gcttactccc tttacggtgc ttactttctt tgaaatatgt tcaagccatg agatccatgt
    21421 tttttctttt tcaggatgat ttgttttaac ccatgcttca acggctgcaa gcatacctat
    21481 atgctcctcc cttcctatct tgttgtccct tcccgggcca tgatgaggag aactggcctg
    21541 ccatgtggaa aaaagaagat cctttttacc gagaagaagc cccgcgccct gaggacccct
    21601 gattgctttt cctccgctgt aggcaacaat ggtcgcaccc cgttccaggt gaatgttcgg
    21661 aacggtaagc acttcagctg ccgcatccac cagaatgggg atatccgtat ccttaagggc
    21721 tgctgcaata ttgtctattg acaggggccc ttcactgtaa tcccttggag ccgcaaaaag
    21781 ataaaccatt gcagtgcgcg gactgatggc ctttttcagt tcttccatgg tctggacctc
    21841 aaccattgtt acgcctgcat tcctgatggc tgcatcatac tgattccttg aatagcgcgg
    21901 aatgattacc tctgttttgg gaaatccggt aaggtcaggt actcttacaa gcttttcagg
    21961 atcgccgccg gtgatacagg caactgttac atgtttcatt cctgcagcac agcctgagga
    22021 taccattccc cattcagccc ctgtaatttc tgccagcctt ttgcccacag ccattgccag
    22081 ttcatcaata tatacatagt tcagggcagc atattcaatg gctttccgta cctccggcaa
    22141 tggaatggag cctccgattg ctgtgaaggt tcccatgcag tttatcaggg gttcaacacc
    22201 tatggactgg aatatctggg gtccgggagc cagggcgcct tcaggggcat tggcaagaag
    22261 accggcttcc tttgcacttc gtttttgtgt ggctgatgcc atcaggggat ccagagacat
    22321 taaacctcct gctaccggaa tggcagacag ggtttttatt acttccctac gtttcatagt
    22381 atttttgttt aaagattttg aatttaaaat attaaatgga aaacaaataa tttgtatgta
    22441 aaagcacttt aaatctcctg aattggataa aagcagaagt gtttcacaaa aatatcataa
    22501 agacggaact tatcaaactt ttgtgatatt cttctgatgc cggaacatgg ctgttccggc
    22561 ctttggctgc ccagaggcaa taaaacggaa aaccggggct gaggaataat gaaaataaag
    22621 acttgctccg gccggtcaga aaataccggg ataatatgtt tatatacagc tgcctactga
    22681 ggctcgtaac tgccgggagt tgttctgaaa cttatggcca ggcggttcca tccgttaatg
    22741 gcgatgatgg ccatggtaag cgctacaatt tcttcttcac tgaaatattc catggtagct
    22801 ttgtacaggg aatcggatat ctcattctct gaaatcctgg tcagagcttc tgtccaggcc
    22861 agtgctgccc tttcacgttg agtgtaaaag ggtgtttcac gccaggcgga cagtgcataa
    22921 agacgctgtt cggtttcccc ggccctgcgc gcatcctttg tatgcatgtc aatgcaatag
    22981 gcacagccgt ttatttgtga tgccctggtt tttacgagtt catacagcga cctttcaagg
    23041 ccggagccat ggacatactt ctcaagttcc agcattccct tcagggcgcc gggtgcagtc
    23101 tttgaataat ccagtcttgg cttcatgagt gaaattttta tttgatttat ttatctgttg
    23161 attcgtttaa ttccttctcc agccggagcg cctcaggaaa aagaatattg ttttccaggt
    23221 gaacatgcct gtgcaaatcc cgttcaaaat cctccagctg tttaaaggct gtccggtaac
    23281 ttgtgcaggc atcggcaggc ggactgtagc ttcccgatat ttccgaaatc ctttcaaacc
    23341 ttcttccttc gttctcatgc tcggaaatca tcaccctgac aggattggcc accgtaccaa
    23401 aagcctcggg cccgggccgg gaaccctgct tttttgattc caccatttcc cggatgaaag
    23461 ggaaaagcat gacttcctct ttctgcatgt gagctgcaag atctgaggca gactcattga
    23521 acagcttcct tatttcaaaa agttccggat gagcagaacc gtggacatca cagattttgt
    23581 ccaggttttc cctgatatgg ggaatattct tttttacgta ggaatgatgc ctccttacaa
    23641 tataatcact taactcgtca ggagccagac tgttgatgta ttccgtatca gggtcggaat
    23701 ttcccgctat atcttccagt tgcgaaatca gacggtctgc gtcaatgccg gcatcactgc
    23761 aggcctcgga tatcgttttt tcgcccccgc agcagtaatc aatgttgtat tcccggaata
    23821 actgtgctgt tctgaaattc agccgtacaa cttctcctat acctgtgttg cctgaaataa
    23881 tcatggtggt tttttaaatg tttatgaaac aaaggtatac attatttata caataaagac
    23941 aaaaagatcg acatatattt aattatctgc ttacttttcc atccggcaga tattgagacg
    24001 gatttttccg cggtgcacat gataattctc ataatttttt cgatgcgccc ggaaaagtta
    24061 atatattgaa aaaaatttaa aaccatatgt ccaggaacct tagaggcctc tcactgagaa
    24121 aaggccttac cgacagcatg tatgaacaaa tctcggatgc ggcactgggt gctgatgatc
    24181 tgagggaagc taatctgata tcggttgccc gtgagttcaa gacggggaat gccgccattc
    24241 tgagtgcaag ttccttctat gatttccttc agcccggaca tcagggcaaa aaggtttttg
    24301 tatgtgacgg aactgcctgt cttgtatccg gcaggacgga agaggttatg cgggagctgc
    24361 tcgaaacata ccagccgcat gagattggca cgatgacctg cctcgggcac tgtcatacca
    24421 acgatgccta tattgccggc ggggaaatcc gctactgcaa atgcggagga ggcagggaaa
    24481 ctcctgaagt gtattccaat gcggagaacc ctgtactgat tgttcccacc ggtgatatca
    24541 gggactatta ttcccccctg gccgctctgc gcaataatac cgaatctgcc ctccgggaac
    24601 tcgggagttc cggacttcgt ggccggggcg gtgcaggatt tccgttccat atcaaggtcc
    24661 gttccgcgct gaatgcagct acagccgata cgaagtatgt tgtctgcaac ggcgacgaag
    24721 gagatccggg agctttttcc gacaaatggc ttcttgagga acgtccgcat tcagtgcttt
    24781 tcggaatgct tgccacgggt attatcatcg gagccggtgc aggggtggtc tatatccgcg
    24841 gggaatatcc cctggctata aaaagggtaa aggaagcaat ccgtacactt gctgaactgg
    24901 gaattgctgc cggggaggaa aacgatgaca gcggcctttt ccgctttcat gttgttcagg
    24961 gctcgggggc atatatatgc ggtgaggaga cctcactgct taactctatt gagggattga
    25021 gggcggaggt ccgcacccgt ccgccttttc cggctgttta cggactcttt ggcaggccga
    25081 ctgttctgag caatgtggaa acctttgcca atgtccgcta tatactcgaa aggggaggtg
    25141 ctgcctgggc agcaatggga accgcgaaat cacccggcac aaagcttgta tctcttgatg
    25201 gtgcctttaa caggccggga ctgctggagg tgaagatggg gactcccctc agaaacgtta
    25261 tggacgaaat gggcggggga acacgctatc cggtgaaggc cttccagata ggcggcccgc
    25321 tgggcggact ggtacccgca tcaaggatag acgacctgac gctggatttc gaatcctttg
    25381 ccagggaagg atttctgctc ggacatgcag gggtggtgtc aatacctgaa gatttccctg
    25441 taatcagact ggtagagcat ctttttgaat ttacaagaaa ggaatcctgc ggcaagtgct
    25501 ttccctgccg gctgggttca gcgcgggggc atgacctggt ttccgctgca gtgaaagacg
    25561 gagagcttat tgaccgtcag cttttcgatg atctcctgga agcgatggag ctcggatctc
    25621 tctgtgccct gggaagcggc ttgcctctgc ccgtaaaaaa tgcattacag tattttggtg
    25681 atgaactgaa ggactatttt acaaccataa aggaatcata gccatggaaa agaccgcata
    25741 tatagacaat aagccctatg caataaggga cggagagacc atcctggagt ttgtgcgcag
    25801 gaatctcgga cgcgaaatga ttccgacact gtgtcaggct gacaatcttg aaaactatgg
    25861 ttcgtgcagg atatgttcgg ttgatgtggc ccttgaggaa aacggcccgg caaaagttat
    25921 ggcatcatgc catacccctg tgatgccggg atattatatc tatccttcca ctgacaggat
    25981 aaggcgcctc aggaaaaata tacttgaact ggtattgtcg gaataccctc ctgaaaggct
    26041 tgcacccgaa gccggcatgc ttcccacgga attccagtca gttattgcaa cagccggatc
    26101 tcctgaagta cggtaccgcc ggaaaataag caaatatggg aaagacagga cccggcccta
    26161 tgtatggtcg gatttcacgg aatgcatcaa atgctaccgt tgtgtgagag cctgtgatga
    26221 actgcagacc gagcgcgtgt tgtggataca tggaagggcc gacaggagcc gtatcataaa
    26281 ggattttgac aggtcattca ttgaatccac ctgtgtttca tgcggagcct gtgtacagac
    26341 ctgtccgaca aatgcactct cagaccgtta ttccgccaag acccttgcgg cagacaaaac
    26401 cgtacggact acctgtacct actgcggtgt gggctgcaac ctggatgtaa aggtgattga
    26461 cggtgccgtc agggctattc aggctcctct tgacgcagcc acaaacaggg gacatacctg
    26521 cttaaaggga aggtatgcat ttgagtttta caatcatccc gacaggctgc gctccccgat
    26581 gataaggaaa aacggaacgc ttacggaggt atcatgggat gaagcctatg aatataccgc
    26641 aaaacgcttc cgcgaaataa gggaaaagta cggaccggat gcccttgccg gaatttcatc
    26701 agccagatgt acaaatgagg aaaactatct tatgcagaag ttcttcagga tagttatcgg
    26761 aaccaataat attgacggct gtgcaagggt atgccatgcc ccgacggcaa tgggcatgca
    26821 gtgggccttt ggtaccggag cggcaacaaa ctccattgac gatatgtaca ggaccggatg
    26881 catgctggtt atcggagcca atccggttga tgctcatccg gtgacaggtg cgcgtatcaa
    26941 atccgtaacc gaatcgggta ttccgctgat cgttatagat cccctgaaga taggactggc
    27001 aagggttgca aagcatcacc tgcaaatcaa tccgggaaca aatgttgccg tacttaatct
    27061 ctttgcctat tacctgctga aagagggtct tgctgatgaa gatttcattg cttcccgtac
    27121 cgaaggctgg gaggatttta agaattacct tgaaacactt gatgttgatg aacaggagag
    27181 aatctgcggc gttgaccgtg aagaggtgag ggctgcggcc attgaatacg ggtcagcatc
    27241 cgaggccatg gaatttcacg gactgggagt gaccgagcac tggcaggcaa ccaaggcaat
    27301 aacccttata tcaaatatcg ccatgatgac aggaaacatc ggcagaccgg gaacgggtgt
    27361 caatcccctc cggggtcaga acaatgtgca gggtgcagcc gatatgggag tccagccaca
    27421 tcagggagca ggttatctcg atgtaaccaa tccgcagaac attgaacttt atacctccta
    27481 ttacggaatt gaacatcctt caaaacccgg actgcgtatt ccggaaatgt ttgctgctgc
    27541 atcaaccggt gaacttaagg cattatggat catgggcgag gacatcctgc agactgaccc
    27601 caatacctgc catgtgagga gctcactgca gggccttgaa ttcctggttg tccaggagat
    27661 attcatgtct gaaactgccc gtatcgccga tgtgatattc cccgcttcat ccttcctcga
    27721 gaaggaaggc acctttacaa acggggaaag aaggatacag aaagttcaga aagttgttga
    27781 cccgcttgaa ggtaccagac ctgacggccg gattgtggtt gaaatgatga atcacatggc
    27841 ttatccgcag gctgaataca cagctgcggg cgtcctggaa gagatagccg gaatagtgcc
    27901 attcttcagg ggcgttaaat gggaggaact ggggaaaaac ggcaagcaat ggccggttct
    27961 tgaagatggt acagacacca ggataatgca cgtgggaaca ttcaagagag ggaagggcaa
    28021 attccattcc tgggattatg agagttcccc tgagctcatt gaacactctg cggaatatcc
    28081 ctacatactg accacgggaa gactcctgga acattataac agcggcacaa tgacgaggcg
    28141 cacgcccaac agcgaactgg tgactgaaga cctgctgtat gtaaatccgc atgatgcgca
    28201 cgcaaaggga ctggtaagcg gtgatttcgc acggatattc tcagcccgcg gaataactac
    28261 catgaaagtg gaagtgacgg atgtggttaa acccggtgtt atctacagca cattccattt
    28321 ccccgaggcg ggaataaact ttctgaccag cggaataggc gatgaataca ctcttacgcc
    28381 ggaatacaag gtggctgccg ttgattttga aaaatcggtt tacgggatat ttgcacgtgc
    28441 cgaatgccgt acggatctga aatagggttt tttcaggtgc agtaattatt tctgactgat
    28501 cctgtatgcc ataagggctt tacccctggg tatatacaaa actccctcat ggattacagg
    28561 atgtgcccag tgaggtcccg accccccgcg gacagtcatt ttactgataa tttcaaaacc
    28621 ggaaggatca ggtttcacaa gagctgcttc acccttttca ctgaagcagt atagcagtcc
    28681 gtcagcagat atcaccgcac ccatgaacag cgaccgtgag gaatacatct tttctcccgt
    28741 ggtccagttg tagcactgcc atgaagggtc attgtgccct gaaccgtaca ggtaaccatt
    28801 aatcagaacg gcagcaccca tacggttgtc gaaggactga ctgaaccact gcctgcttac
    28861 cgaggtgccg tcggggctaa gcctgagcat tactgccccc atgccatagc cgctgagaca
    28921 gaaaagcagc ccgtctttat aaagcggggt attctgatgt tccagccgga gattggccca
    28981 tggatatgac cacagaagat ttccgtttct gatatcgatt cccagtatgt gtgattctgt
    29041 gtgtgtgacc agcagcgtac gcgaaggaag tcttatcacc gcaggtgtgc agtaagccgc
    29101 cttctcaccc atgccgggac atgtccatac gagatcaccg gtgaaacggt tcagtgcaat
    29161 gacgttgttc ctgaccccgc cgggagtagc gtacagcctg tctccgtcaa caagaaccgt
    29221 ttcattatag ccataacggt ttatcacacc gtcaaagtcc cttgtcagtt ccctgctcca
    29281 tctgagggat ccgtcccctg tatccat
//
LOCUS       DAOSPX010000007        26444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2038_length_26444_cov_4.700140, whole genome shotgun sequence.
ACCESSION   DAOSPX010000007 DAOSPX010000000
VERSION     DAOSPX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26444
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2038_length_26444_cov_4.700140"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1792
                     /locus_tag="PLZ75_00820"
     CDS             377..1792
                     /locus_tag="PLZ75_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOS70971.1"
                     /translation="MSNQRSLSGTLGLGYVIIFVFANIIGSGVYKKIAPMAAGLESSA
                     WILLAWAAAGVITLFGALSNAELAGMLADTGGEFVYLRKIYNPFFSFLYGWTLFTVIQ
                     SAAISSLAYVFVQSLNSIVPLPELFPSLQDAGIGGIFYPFRDFGIKITAILLIMGLTF
                     LNISGLKSGSRVNKVLFFVVCAGLALIVVSGLANASGRPSEIIDFGSMKESRVSVSSF
                     MTAMLAAFWAYQGWVSAGFIGGEIKNARRNVPKGIIIGVFSVTIIYLLVNYSYLAVMS
                     VPELVSVHEAGNQVAAIEMAGRVGGSSGIFLMSLLILVTTLGCLNASILTGSRPYYAM
                     SREGLFFTAIGKLNSRNVPGNSLLWQGIWSSVLVLSGNFDQLTDMLVFAVYIFYGAMA
                     AGVFILRYRMPDAFRPYKVWGYPLVPALFVLFCAGLFVNTFITRPREALIGLFLIMTG
                     VPVYFLFRRRNGKRASARPHD"
     gene            complement(1811..2836)
                     /locus_tag="PLZ75_00825"
     CDS             complement(1811..2836)
                     /locus_tag="PLZ75_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOS70972.1"
                     /translation="MKRILIFLIVSVPILLTSRSCIDTIDFRGKEMESKIVMYSLLQP
                     DSTIKAFLSFSRTLFGNTSPQQQITNAVVKLYRDGEFIETLTYVPPSVQTWYPEPTPF
                     SEYVSAGETKPLPGSVYRIVAEIPGFKTAAGSASLPGIVPIEGIDTVSYTRDEFYMYL
                     GIKTRFTDPAGKDNFYMISGYQKTGYYSGNPYQPWNPEIPVRVFEYEMDINADDEPLI
                     YPFMNDDIFNIYPYNYFNVFSDEMIPGKGYELNLKTDYRKVDTTYYEFFHPTVILQSI
                     SEDLYLYLLSFSKYLDSYDDFFSEPVLVYTNVENGLGVVGAINSSKMTVEIGKYPVDG
                     ILYSYGD"
     gene            complement(2848..5271)
                     /locus_tag="PLZ75_00830"
     CDS             complement(2848..5271)
                     /locus_tag="PLZ75_00830"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS70973.1"
                     /translation="MRLRMFAFLILQIIALQVPAQKVTVSGYISDAGSGERMIGAAVY
                     EKTSLTGSTSNNYGFYSLQLPRGDQEIIVSYLGYEQESVLVNLTSDTIINFNLEQSVR
                     ELETVTITAYGSQNKIKSSVVSMIDIPIEKFRMMPVLLGEADVLKIIQLLPGVQSGTE
                     GSAGIYVRGGGPDQNLFLLDGVPVYNASHLFGFMSVFNPDAVKSVQLYKGGFPARYGE
                     RLSSVVDIRTREGNEKEFHGSLSVGLIASKFTAEGPIVKDKTSFIISGRRTYADILAQ
                     PFIAMGNKDPNYKSNGGYFFYDLNAKINHKFSEKSRLYLSSYLGKDKAYLRYKDTPDY
                     YEDDGHKYEYTDRIGMGWGNIITSLRWNLLISNKIFSNSTLTYSNYKFDVTTEYLTRD
                     ITAKMKYHDYFRYFSGIEDIGVKTDFDYYPGANHTVRFGAGYTYHHFKPGVTRISFSA
                     SDLAGGIDTTFGDQKIYAGELIAYAEDDFDITARIKINAGLRLSLLNVQDAGYLSLQP
                     RVSLRFLATDDLSFKASYSRMTQFVHLLTTSSVSLPTDLWLPVTRKFRPPLSDQIAVG
                     SYFRLPWQLDMTIEGFYKSMDYLIEYREGASFTGSATGWESKVVNGKGWAYGVEFLIE
                     KSLGKTSGWIGYTLSKTLRKFDELNFGRTFPAKYDRRHDVSIALTHKFNERIDIGSVW
                     IYGSGNAVTLGIMEYSPMDYFIPRYFRDEKITEFPYRNNYRAPAYHRLDLSLNLHKKK
                     NKGIRTWNFAIYNVYNRRNPFFIFWDDEYFEEADPFNPGMTVTTARPVLKKASLFPII
                     PSVTYSFIF"
     gene            complement(5426..5989)
                     /locus_tag="PLZ75_00835"
     CDS             complement(5426..5989)
                     /locus_tag="PLZ75_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70974.1"
                     /translation="MRKLIFLGAAALLISAGVTAQSSEPENRVKTKKEIRIEARQQVR
                     ADEQAPQTDPLTTRKQTREQLRTQDPSGDPIMQQTRSREKIHQQAPIGDPAMKQTRSR
                     EKIHQPDPEGGQAGRANRAATYGKRPGAAANKNHSGKSQAVNPPAKAPGQTRGPAMQA
                     RPAAKMQGSTKGIRTHRPAGRGNGAGR"
     gene            complement(6064..7374)
                     /locus_tag="PLZ75_00840"
     CDS             complement(6064..7374)
                     /locus_tag="PLZ75_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS70975.1"
                     /translation="MKLQTKLALFNLLSKLTFSALFIVFLPLIIEHVNIRLSDNDLIY
                     KREEVINLISEVGIEPFINIDNATSFGSYNILKEEYISLEKADDEEEWNYIDVTRRSI
                     DDEVIDYRVLHYSLKVDGVSYLLEIGRSLSSITASARHFRNVILVFLALFIAVTLFSD
                     LTYTARLLRPLKVITGRLGDASSPSFYTEGPLITSTDDFIQLDNTLRKMMKKISELFR
                     KEKEITVNISHELLTPVSVLRSKLENMLLTENLDPATETAIEESLRTLHRLKTLVNSL
                     LMIARLESNQYLREESVDICELVREVTGELAPMADDSRVSITLSCEAVYIMKNANRSL
                     LFSMLYNVVNNAVRHTREGGAVEIAARRKEGRLEVSVCDNGEGMLPEQLNRLFTRFSS
                     RSGKKGENTGIGLAITKSIADFHAIDIRVLSDPGKGTQFFFFFP"
     gene            complement(7389..8072)
                     /locus_tag="PLZ75_00845"
     CDS             complement(7389..8072)
                     /locus_tag="PLZ75_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS70976.1"
                     /translation="MNILIVEDEKSLAGEIARYLTRENFLCEIAHTGAEASEKIAVNL
                     YDFILLDLGLPDYNGIDLISEARKISSESAFIIITARGAVEERIKGLDLGADDYLPKP
                     FALAELHARIMAVARRKFRINSPDIPLGSFTLIISARKLMSGDREVQLTKKEFDLLYY
                     LAINRNKVMTRHQLYEHIWGNILDDQYDSNFIDVHIRNIRKKLNAHSPSPWLETVRGV
                     GYRISNETK"
     gene            complement(8082..8987)
                     /locus_tag="PLZ75_00850"
     CDS             complement(8082..8987)
                     /locus_tag="PLZ75_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70977.1"
                     /translation="MIVCILAVLTLTPHSTAQSNPASAFTPHAGANDSVAGNRLSSLY
                     AGSGYGNNLLYLGTTMSQGNSFGYVSAAYGLKEKLYLSATTYTISGLSPFIAFYSLDL
                     SFSHTFNSWFDINTGFSRYNVAEKLRDTLFGNFSYAYATLGFDWRILYTQLSAGLFMA
                     DGTLPYVQARFSRYFETRSFARDKAFFSIDPYVNILAGSTFHTESSQGATGTGMNQGT
                     GTGAGIGSGTWKRERKGRNHPTGTTITSSFGLIELDLGLPAAFSYDFFTLELEPGYII
                     PLYPGKELPRNGGFLFQVSLFFRIF"
     gene            complement(9035..9550)
                     /locus_tag="PLZ75_00855"
     CDS             complement(9035..9550)
                     /locus_tag="PLZ75_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70978.1"
                     /translation="MKLKKIGKSIVMLLIIIPVSSLITVNAQELKAGRDPVMLQRQQM
                     EQKEQEQKAQEQKRAEQTAQEQKAQEQKTQDRLRTRDQTNGENQVRLGGQTGERANAS
                     GSQSQTAPGGKRGSQPGGTSAVPAKKIQGARPDWSKAKGARPASIERPSGSRIPKGAG
                     RPAGARGPGRR"
     gene            complement(9831..11324)
                     /locus_tag="PLZ75_00860"
     CDS             complement(9831..11324)
                     /locus_tag="PLZ75_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS70979.1"
                     /translation="MNRINIRIIFWTILLYGLIGAQANAGKIIYPWRAVPAIVKCGNN
                     FSILYDNERSILIDSVILEGPYNKIILNIDSVLIGKFEYDPYSHSSTNNEIWVTIPDS
                     TPEELYDLIIKSGGEVQRSLKSVKVVKEFKPKHSFIHISDLHVSRQWVGTPVNGYAKE
                     LELLNGFLKVANIISPDFILVTGDNIHNYTRINADATGWGGTLITDAGSRPLVEEKWR
                     NYFEGANNFLGINGFNSPVFSVTGNHDFYGVDENDHKAKVTQWNSLCGKRIYGFSYGE
                     TRVIAADDFMGDPVTDIPATSPMSGLQGRFFTSFFNQNGSGLLRIMAQHRHDRIDTSF
                     CNKYKINILLHGHNHKPFVESVGITPTLSTRPGAVCRSGVLDTEAELGFFRLFYIDGD
                     KFTYTEPLRFCKDPTKSYNELDLNLTIDFEKPNNGSSDKNKATLNNKLGVDLSDCNIR
                     FIMKKGKYQVTKGFIFQVIETNIYSIVDVRANIPNGKLTDITISTLK"
     gene            complement(11473..13140)
                     /locus_tag="PLZ75_00865"
     CDS             complement(11473..13140)
                     /locus_tag="PLZ75_00865"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS70980.1"
                     /translation="MSIKHLLIAITTVFIFVSCNESWLSEKPPHLITTETLYTNLAGF
                     ETGLNGLYGMVRMEREGRTGANDLIADMFMNGVDNLVTNHRDVGFSMICERWGDLNNP
                     ENAHYIGVFEWLYSIINAANTIINQAEKKDNIDWSGGGFPAEENKNRVIAEAKAIRGW
                     AYRHLTYCWGDVPLSLEESLGSTIKTNWTRTPTADVRKQIISDFLFAEKYIPVEGSLQ
                     GRITKGAVQHYLSEMYLVKNNPDSSLYWADQVINNPAYQLITSRYGVKSNQPGVPFMD
                     MFYEGNENRNQGNTEALWVFQFAIFTNGGGKCLMRRHHGSRYAEINISGVRPLQDTYE
                     RGGRGRSRMSFSKWALELYEPQDDRGSHYAIRKFFILNNAEDNAPYPADRLPPGYQYG
                     DTIWLDWSNDITYETRARLNWPFSNKTNGTDPSNVSDSYQYNDQIYLRLADTYLLKAE
                     AQFKLGLSEEAANTINIIRRRSNASDITAGDVNIDFILDERSRELILEEHRRYTLIRT
                     HKLLERTRAYNNNGGGLISERDTIFPIPQPVIDANISQSMPQNPGWN"
     gene            complement(13176..16103)
                     /locus_tag="PLZ75_00870"
     CDS             complement(13176..16103)
                     /locus_tag="PLZ75_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS70981.1"
                     /translation="MKKGLLIILLLIASLFLNAQKITVSGVITDSQNTPLPGATVQVK
                     GTTTGVLTDANGRFTVDVSSSADILVISFVGYNSEEVIVGNQKTINVILSENILGLEE
                     VIVVGYGTQKKSDVTGTVASLPKDRLEMVPNLNIAQAIQGAVPGVMIQTSSAGAASNE
                     VILIRGRNSIKASNDPLIVVDGVPYGGQISDINTNDVQSIEVLKDASSAAIYGSRGAN
                     GVILITTKEGQEGKLSISYEGKYSVQDFINMPDIMDGREFHDFKLTRHPGILLTQTEQ
                     NVYDSGNWVNWMNMILQKGHSQQHNLSFSGGSKNTSYYIAGSYIDVQGLIVNDNYKRI
                     TNRINIDTKFNNWLSFGTRTQFSFDDQSGRSPSIQAGWQMSPLSIPYDNAGNLTIYPW
                     PEDTYFQNPLAPTLYKDVDKSHQIVLNNYAAIDVPFIPGLNYRLNVGFRFRFLDKGYY
                     AGRDTKEGLEARGRASTDRNQFNSTVIENVLSYSRTFGLHNIFATGVFSFEGNKTSSN
                     SMSASGFPHDFLTWYSSSQAELIVPAYSYNENYLISQMLRFNYSYGSRYLVTLTSRRD
                     GYSGFGTKHKWGIFPSFALGWNISNESFFPWKNIINELKLRASIGYNGNQAVSAYESI
                     SRLSQFNMVSNKTTVPGYRPSKLGQDELGWESSRSINLGLDFGIYKNRISGEINIYNT
                     NTTDLLLDRSISAVHGITSITQNIGETRNIGLEVSLNSRNIIKNDFSWTTSGNLALLK
                     NKIVSLYGTLDEKGKEIDDVANEWFIGYPIRVNFDYVWLGTWQLEEAEEAASWGSQPG
                     FVKLWDADGDKTLTAADRRIIGQRDPKFLWGLNNSFTYKNFGLDIFVHGIHGVTKRNT
                     LMADATFAETRQSTTKKDWWTPTNPTNKWVMNHYDATRMGGITAAYYEKADFLRLKDL
                     SFSYNFSKNVLEKLKITNLRAFITGRNLLTLTKWSGLDPELDSQTSTPLQREFVIGLN
                     TSF"
     gene            complement(17165..17773)
                     /locus_tag="PLZ75_00875"
     CDS             complement(17165..17773)
                     /locus_tag="PLZ75_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS70982.1"
                     /translation="MKKFSWLFAILLMASVSAEAAGPYQPGDYASDFSLKNVDGKMVS
                     LSQFRDARGFIVIFICNTCPVVRQYESRIIDLHKKYADKGYPVIAINSNDVNTSPGDS
                     FEEMQKFAAEKGFGFNYLYDETQEVLNKYGATNTPHVYVLSKKDGNNKVEYVGAIDNN
                     TENPDLADKKYVEDAVNSLLRAEPVAITKTRAIGCSVKLKKA"
     gene            complement(17799..18233)
                     /locus_tag="PLZ75_00880"
     CDS             complement(17799..18233)
                     /locus_tag="PLZ75_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOS70983.1"
                     /translation="MRSQEISYINIPDLDKLLKAPENKLYVVNFWATWCAPCIAELPG
                     FQKISKEYDPEKVSFIMVSLDFPGQVQKQLVPFLKKNMITLDVRVMTDLDYNAWIEKV
                     DPSWQGNIPATLFFNNSRKTRFFHAGEIDESGLRKFIDTCLN"
     regulatory      18459..18639
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            18731..20935
                     /locus_tag="PLZ75_00885"
     CDS             18731..20935
                     /locus_tag="PLZ75_00885"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOS70984.1"
                     /translation="MNGKLFFSQIIKRNGDVVPFNREKIENAVFKATRAIGRPDRAKA
                     NRLSADVLEKLSKLEIRELPTVELVQDQVERTLFEDGDFQLAKAYILYRKQRELARNA
                     QELFSNIEVVDDYLGISDWRVNESANSAYSLQGLNQHISTTITSQYWLDRIYPENISS
                     AHKQGYFHIHDLGFLSVYCVGWDLRDLLLSGFRGVNGKTQTKPARHFRTALGQIVNFF
                     YTMQGEAAGAQAFSNFDTLLAPYIRKDNLDYKQIKQCIQEFLFNMNIPTRVGFQTPFT
                     NITMDLHVPDFLKQEQIVWGGEIQEDVYGSFQNEITLLNKAFAEVMLEGDAAGSLFSF
                     PIPTYNITPDFDWDNSDYDSIWEMTAKYGIPYFSNFVNSDMKPDDVRSMCCRLRLDKR
                     ELIKRGGGLFASNPLTGSVGVVTINLSKLGYEAISENNFFEKLKNLMILARESLEIKR
                     KTIENYTEKGLYPYSKFYLRDIYKRFNCYWKNHFSTIGINGMNECCLNFLGKDIGHPE
                     SKSFAIRVMDFMRNVLEGFQEETGNIYNLEATPAESTAFRFARIDTIQYPDIITANQK
                     AYKEGAAPYYTNSTHLPVGYSDDIYEVLSLQDDLQVKYTGGTVLHVFTGDTNMPAMSA
                     KNLIRKITKAFHLPYITISPTFSICPSHGYIPGEHFSCPECNSETDVYSRIVGYMRPV
                     NQWNKGKKQEFKDRKLFRPDTGNKEMEEDQGMEENQGIPEKASLIPELNKSR"
     gene            20935..21579
                     /locus_tag="PLZ75_00890"
     CDS             20935..21579
                     /locus_tag="PLZ75_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOS70985.1"
                     /translation="MRIGGFLKHSLIDWEGMITAVIFTRGCNFRCGYCHNPSLVLPGL
                     FNETEDIESEEILSYLNIRKQWIDGVVISGGEPTVHPDLPEFIKLVRNAGYRIKLDTN
                     GSNPVMLKELVSAGLVDFVAMDIKTLLKSSAYYEITGSSSLRLIQRISESVSILRTSG
                     VQYQFRTTLVPGFHTEEIVEQLKKEFKGDPLVLQQFREGVTIESLNDRQSFPNS"
     gene            complement(21569..22642)
                     /locus_tag="PLZ75_00895"
     CDS             complement(21569..22642)
                     /locus_tag="PLZ75_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70986.1"
                     /translation="MKKIRLKTLFPAFALITVLLLVSSCEKTVDDPPITINNLKGMFV
                     VCEGVFGQANGDISFYDSKTGQTTKNLFYSVNGMELGDVVQSFVIADTLGFIVVNNSQ
                     KVTVVNMKDFKLVKTIDGFSYPRSIVRADENTLYVSNGNGSSDNFIYKINLSTLEISG
                     SLALSTGPEKLIAAGSKVYAAISGGWNNDGNTVIQIDPVTFSVTGTYTVASVPVDLVS
                     DKDNNVWAYCKGIADYTNWPEVSYTDAGISRINISANTVSSFPVSTMSAPGINNIAAG
                     KDGSTIYFLNDALYSMPVTATELPDTKVADQEFYGLNVDPESGNIVCLDAVNSKAVVY
                     NTAGVKQLEFETAKFPCSVVFSY"
     gene            complement(22673..24598)
                     /locus_tag="PLZ75_00900"
     CDS             complement(22673..24598)
                     /locus_tag="PLZ75_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS70987.1"
                     /translation="MAEMIRKALLYFRSVLPAILLIITYSTVMFSQSIADTIIQIPDV
                     VVRASRFQQFRSDIKTDEFTSEELSPFAGESLGRFLISKTALNVKSYGVGGALSNISL
                     RGTSTSHVQVNWNGFPINSVTLGSSDFSFIPVSGFDNISVVYGAPGALYGSGTFGGAV
                     NLDNNLKTVKALKGSVNISYESIETINGSLSFTTGNNRLAWKADAWGALSGNEFSYYD
                     YIRQTDRKQTDGQWHSTGTVQQFIFRLSPSSTIEAGFWYQVKAFNIPSRIGSVSWEHQ
                     RDSTLKFFTAWKKSGHRWSLEAKTAVFKDHERYTMKASEQAESYSIDSRIRSGQYYGD
                     VNFRYHAGRNISVDAGLIGTFINADVSAYGHRKEERGLTAFAGIKYDLDRFSTQAGLR
                     KEWNSNFHSGVLSSFGMSREMITDKWKLRLNVSQKFRKPTFNDLFWMPGGNPDLKPET
                     GYSVEAGSSVTVFKKEKAVLSADAGIYWSKINNMIAWRPDGMYWTAMNYQRVYSAGMD
                     CKLILDVKHRKLKYRSALNVMLNRPDVQPYVSGEHEKMLYSPRIITSWENSLSAGIFD
                     MAIDHHFSADRFYDDNSLLKPYQLLDMKTGVKIPVANGHLGIHLSVNNLTNTTYELIR
                     LYPMPGRYWSVKINYTF"
     regulatory      complement(24734..24937)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(25056..>26444)
                     /locus_tag="PLZ75_00905"
     CDS             complement(25056..>26444)
                     /locus_tag="PLZ75_00905"
                     /inference="COORDINATES: protein motif:HMM:NF015347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HOS70988.1"
                     /translation="NQFVTVWNLAIPGGANTEITFNVATRGTVAYTWETIPAAYSGSG
                     TFSGPEATISNLPAGAIIRLNIQPENLERFFIDGGIDRNRLTLVENWGSTAWTSMALA
                     FSGCANLQITAVDVPDLSGLTNMALMFQYCTALDSPENINTWNTASVTNMSYMFAGAT
                     SFNRDIGSWNTSSANDMSGMFRNANAFDRDIGNWNTAAVSSMSEMFAWAYSFNRNISS
                     WNTSSVNDMSGMFQGTIEFNGEIGSWNTGAVTSMNAMFAGSAGFNSDISSWNTSSVND
                     MRDMFVEASGFSRNIGSWTLNPGVMLEYMFDGSGMSCIDYSATLAGWSANPNTPLNRV
                     LGAADMQYGTAAEAARTYLTETLGWTIEGDSFANESCEGLITGITDTNSGELKKITAW
                     MERDEIVINGITEKAVALLYDLKGNAIWIRKIGETGTNRINVNGLITGIYVLLVNENG
                     KDSSIKLFIPGN"
BASE COUNT         7168 a         5727 c         5442 g         8107 t
ORIGIN      
        1 ccagatagct atactccact tgcccggcct gctgctctgc ctgctgaata aactgtgaac
       61 aaccggccct gagaagcaat atcaataaaa tctgtaaaaa actgatcata acttattttc
      121 tttatcctgt ttgtcaatga agcctgattt ccccggtggg aaagaatatc ctgttcatcc
      181 ggcaccatga attccggcgg accatttaca tgtatggatt agtctgcctt cgtcttttgg
      241 tgcgctaaaa ctatcttttt ttcttcattt gtcaaaaaat aatattgcca tcaatcagta
      301 ctgtaggatc agagcataat cctcattcaa cttttttctt aatttggtca attaaaattc
      361 cggattaccg gtgccaatga gtaatcagcg aagtctgtcc gggacactcg gactgggtta
      421 tgttataatt ttcgtctttg ccaatatcat cggttcaggc gtgtacaaaa agatagcacc
      481 gatggctgcc ggcctggagt cgtccgcctg gattctcctg gcatgggctg ctgccggagt
      541 gattactctc ttcggagccc tgagcaatgc cgaactggcc ggaatgctgg ctgacacagg
      601 aggggagttt gtctatctca gaaagatcta caatcccttt ttctcctttc tgtatggctg
      661 gaccctgttc actgtgatcc agtcggctgc catttcttcc ctggcttacg tctttgtcca
      721 gtcattaaac agtattgttc ctcttccgga gttattcccc tcattacagg acgcgggtat
      781 cggaggaatt ttctacccct tccgtgattt cgggataaag attactgcga tcttgcttat
      841 tatggggctg acttttctca atatttcggg actgaaaagc ggatcccgcg ttaacaaggt
      901 tctcttcttt gttgtatgtg ccggtctggc gctgattgtg gtatccggtc tggccaatgc
      961 ttccgggagg ccttcagaaa ttattgattt cggcagcatg aaggaaagcc gggtaagcgt
     1021 ttcgtctttc atgacagcca tgctggcggc tttctgggcc tatcagggat gggtatcggc
     1081 aggatttatc ggaggcgaaa taaagaatgc caggcgaaat gttccgaaag gaattattat
     1141 tggtgttttt tcagtgacaa taatctatct tcttgtcaat tattcctacc tcgcggttat
     1201 gtcagttcct gaactcgtca gtgttcatga agcgggtaat caggttgctg ccattgagat
     1261 ggcgggaagg gtcgggggat catccggaat attcctgatg tctctgctta tacttgtaac
     1321 aaccctgggc tgcctcaatg ccagtatact gacaggttca agaccttact acgcaatgtc
     1381 gcgtgaaggg cttttcttta cagccatcgg gaagctcaac agccggaatg tgcctggtaa
     1441 ttcactgctc tggcagggga tatggtcatc ggttctggtt ctctcgggga actttgacca
     1501 gctgaccgac atgcttgtct ttgccgttta tatattctac ggggcaatgg ctgcaggggt
     1561 ttttatactc cgttacagga tgccggatgc cttccggccg tacaaggtat ggggttaccc
     1621 gcttgtgccg gcattatttg ttctgttttg tgccggactg tttgtcaata ccttcattac
     1681 aaggccccgt gaggccctga taggtctttt tctcataatg acgggcgtac cggtatattt
     1741 cctttttcgc agaagaaacg gaaagcgggc ttcagccagg cctcatgact gaaaaaacgg
     1801 tttctgccgt ttagtcgccg tatgaataca atatgccgtc aaccgggtat tttcctattt
     1861 caacagtcat ctttgaactg ttaattgctc cgaccacacc cagcccgttt tccacatttg
     1921 tataaacaag taccggttcg gagaaaaaat catcataact gtcaagatat tttgaaaagg
     1981 aaagcagata aaggtaaaga tcctcactta ttgattgcag tataacagtg ggatggaaaa
     2041 attcgtaata agtagtgtca acttttctgt agtcggtttt aagattaagt tcatacccct
     2101 ttcccggaat catttcatca ctgaagacat tgaagtaatt atagggataa atgttaaaga
     2161 tgtcatcatt catgaaaggg tatatcagag gctcatcgtc agcattgata tccatttcat
     2221 attcaaagac tcttacaggt atttccggat tccagggctg atacggattt ccggaatagt
     2281 aaccggtttt ctggtaacct gaaatcatgt agaaattatc tttgccggca ggatcggtga
     2341 acctggtttt tattcccaga tacatataaa attcatcacg ggtatatgat acggtatcta
     2401 ttccctctat cggcactata ccgggaaggc tggccgaacc agctgctgtt ttaaagccgg
     2461 gaatctctgc aacaatcctg taaacacttc cgggaagcgg ttttgtttct ccggcggaaa
     2521 catattctga aaaaggtgtt ggttccggat accacgtttg tacggaaggc ggaacataag
     2581 tcagggtttc aataaactca ccgtcacggt acagttttac aacagcattt gttatctgtt
     2641 gctgcgggga tgtattcccg aacaatgttc tgctgaatga caggaaggct ttgattgtac
     2701 tgtcgggctg tagcagtgaa tacataacta tctttgattc catttcctta cccctgaaat
     2761 caattgtatc gatgcatgac cggcttgtca gcagaatggg aacagataca atcagaaata
     2821 tgagtattct tttcatttgt tcagtattca gaaaataaag gaataggtta ctgatggaat
     2881 gatcgggaag agactggctt ttttcagcac cggtctggcg gtagtaacag tcattcccgg
     2941 attgaaggga tcagcttctt caaaatactc atcatcccag aatatgaaga agggatttct
     3001 cctgttgtaa acattgtata tggcaaagtt ccaggtacgt atccccttat tttttttctt
     3061 gtgaaggtta agtgacagat ccaggcggtg atatgcaggg gccctgtaat tattacggta
     3121 agggaactcg gtgatctttt cgtccctgaa gtaccgggga atgaaataat ccatcggtga
     3181 atattccata ataccgaggg tgaccgcatt gcccgagcca taaatccata ctgaacctat
     3241 atcaatcctt tcattgaatt tatgggtcag tgcaatgctg acatcatgcc tgcggtcgta
     3301 ttttgccggg aaggttctcc cgaagttaag ctcatcgaac ttcctgagcg tttttgacag
     3361 ggtatagccg atccagccgc tagtttttcc caggcttttt tcaatcagaa attccactcc
     3421 gtatgcccat cccttaccgt ttacaacttt tgattcccaa cccgtggctg aaccggtgaa
     3481 agaggctcct tccctgtatt ctatcagata atccattgat ttgtaaaacc cttcaatggt
     3541 catgtcaagc tgccagggca ggcggaaata cgaacccaca gcaatctgat cggacagggg
     3601 tggtctgaat tttcttgtta ccggaagcca gaggtcagtc ggaagactga cggatgatgt
     3661 ggtcagcagg tggacaaact gtgtcattct gctgtatgaa gccttgaatg acagatcgtc
     3721 tgttgcaagg aagcgtaatg atacccttgg ctgcagggat aaatagccgg catcctgaac
     3781 attgagaagt gacagcctta atccggcatt gattttaatg cgtgctgtga tgtcgaaatc
     3841 atcttccgca taggcaatca attcaccggc atagatcttc tgatcgccga atgtggtgtc
     3901 tatgccgcct gccagatcgc ttgcgctgaa actgatcctt gtaacaccgg gtttgaaatg
     3961 gtgataggta tagcctgcac cgaatctgac cgtatggttt gccccgggat agtaatcaaa
     4021 gtcagttttt acaccaatat cctcaatacc tgaaaaatac ctgaagtagt catgatattt
     4081 cattttggcg gtaatatccc tggtcaggta ttcagtggtc acgtcaaact tgtaattact
     4141 gtaggtaagg gtagagttgc tgaaaatttt gttgcttatc agaaggttcc atctcaggga
     4201 agtaatgatg tttccccatc ccatgccgat cctgtcggta tattcatatt tatggccatc
     4261 atcctcataa taatcaggtg tgtctttata tctgagatat gccttatcct ttccaaggta
     4321 agaactcaga tagaggcggc ttttttcaga gaatttgtga tttatcttgg cattcagatc
     4381 gtagaaaaaa taacccccgt tagacttata attgggatcc ttgttgccca tggctataaa
     4441 cggttgtgcc agtatatcag cataagtgcg tcttccggaa atgataaacg atgttttgtc
     4501 tttcacaatc ggtccttctg cagtaaattt tgatgcgata agaccgacgg agaggctgcc
     4561 gtgaaactcc ttttcatttc cttccctggt gcggatatca accactgaag aaagccgttc
     4621 gccatacctt gccgggaatc ctcccttata cagctgaacg gattttacgg catcgggatt
     4681 gaaaactgac atgaagccga aaaggtgact ggcattgtat accggcacac catcaaggag
     4741 gaaaagattc tggtcgggtc ctccgcccct tacatatatt ccggcagaac cttccgtgcc
     4801 agactgcaca cccggaagta actggataat tttcagaaca tcagcctcgc ccagaagaac
     4861 aggcatcatt ctgaattttt ctatcgggat gtcaatcatg ctgacaacgg agcttttgat
     4921 tttattctga ctcccgtatg ctgtgatggt tactgtttcc agttccctga cagactgttc
     4981 caggttaaaa tttataattg tatccgaggt cagatttacc aggacgcttt cctgttcata
     5041 tccaaggtat gaaacaatta tttcctgatc gccccgggga agctgaagac tgtaaaagcc
     5101 atagttattg gaagtactgc cggtcagtga agttttttcg tatacagcgg cgcctatcat
     5161 gcgttctccg cttccggcat cactgatata accgctgact gtcacttttt gtgccggaac
     5221 ctgcagtgca atgatctgca ggatcaaaaa agcaaacatt ctgaggcgca tatgcggtta
     5281 tatctcttat ttgtccgata tgaatttaca aattatttcc tcttttcata ataataaaga
     5341 atttttctct gacgaattat ccttttccgg agttactatt tcccccggct ggtttatcct
     5401 gttggagagt ctgcggttga aggtttcagc ggcctgcccc attgcctctt cctgccggcc
     5461 ggtgggtcct gatacctttt gtggagccct gcattttggc agctggtctg gcctgcatgg
     5521 ccggtccccg ggtttggccc ggtgcctttg ccggcgggtt aacagcctgg cttttaccgc
     5581 tatggttctt gtttgctgct gctccgggtc tcttcccata tgtggctgcc ctgtttgccc
     5641 tgccggcctg tccgccttcc gggtcgggct gatgtatctt ctcccgtgag cgggtctgtt
     5701 tcattgccgg atcgccaatc ggcgcctgct gatggatctt ttcccgtgaa cgggtctgct
     5761 gcatgatcgg atcacctgac ggatcctgcg tcctgagctg ttcccttgtc tgtttcctgg
     5821 tcgttaacgg gtcagtctgc ggtgcctgtt catctgccct tacctgctgc cgggcctcaa
     5881 tcctgatttc ttttttggtc ttaacccggt tctcaggctc tgacgactgt gcggtgacac
     5941 cggcacttat cagaagtgct gcagcgccta aaaatattaa ctttctcatt tttgttctgt
     6001 ttaaatttta ttctcttgtt acaaaacaaa aatgcacccg tgaagatgaa gttacagtga
     6061 aattcagggg aaaaagaaaa aaaactgcgt ccctttgccc ggatctgaca agaccctgat
     6121 gtctatagca tgaaaatcag caattgattt ggtgattgca aggccaatac cggtgttttc
     6181 tcctttcttt ccggatctgc tgctgaagcg tgtaaagagc cggttgagct gttcgggcag
     6241 cattccctcg ccattgtcgc agacagagac ttcaagcctt ccttccttcc tccgggcagc
     6301 gatttcaacc gctccaccct cccgggtatg cctgacagca ttgttgacaa cattataaag
     6361 cattgagaaa aggagtgacc ggttggcatt tttcattata taaacagcct cacatgaaag
     6421 agtaatactc actctgctgt cgtcagccat cggtgccagt tctcccgtta cctcccttac
     6481 cagttcacag atatcaacgc tctcctccct caggtactgg ttgctctcga gccttgcgat
     6541 catcagcagt gaattgacaa gtgtcttgag ccggtgtagt gtcctgagcg attcctcaat
     6601 agcagtctcc gttgccgggt caaggttctc agtaagaagc atattctcaa gcttgctgcg
     6661 gagcactgag acaggggtaa gcagttcatg ggaaatatta acggtgattt ccttctcttt
     6721 cctgaacaac tcactgatct ttttcatcat cttcctgaga gtattgtcaa gctgtatgaa
     6781 gtcgtcggtt gatgtaatca gcggtccctc cgtgtagaaa gatggagagg aggcatcccc
     6841 gagcctgccg gtgattactt tgagagggcg cagtaacctg gccgtatatg tcaggtcaga
     6901 gaagagagtg acagcaataa agagagccag gaataccagg atgacattcc ggaaatgtct
     6961 ggctgaggca gttatacttg acaggctcct gccgatctcc aggaggtatg aaacaccgtc
     7021 aaccttcagg gagtaatgaa gcacccggta atcaatcacc tcatcatcaa tacttcgcct
     7081 ggtgacgtca atgtagttcc actcttcctc atcatccgct ttttcaaggc tgatatactc
     7141 ctctttcagg atgttgtaac tgccgaagga tgttgcatta tcaatgttga tgaagggctc
     7201 aatgcctacc tccgagataa ggttaatgac ttcttcccgt ttgtaaatca gatcgttgtc
     7261 tgagagtctg atattgacat gttcaattat cagcggaagg aagacgatga acagggcgga
     7321 aaaggtcagc ttcgagagca ggttgaagag tgcgagtttt gtctggagtt tcattatacc
     7381 ccgaatagtc atttagtttc attacttatc ctgtagccca cccccctgac tgtttccagc
     7441 catggcgacg gactatgggc attcagtttt tttcggatat tccgaatatg aacatcgatg
     7501 aaattgctgt catactgatc atcaagaata tttccccaaa tatgttcata gagctggtgc
     7561 cgcgtcataa ccttgttcct gtttatggcc aggtaataga gcaggtcaaa ctcctttttg
     7621 gtcagctgca cttcccggtc accggacata agtttccttg cagagataat cagagtaaag
     7681 ctgccgagtg gtatatccgg tgagttgatg cggaacttcc tccttgctac cgccatgata
     7741 cgtgcatgca gctcggccag ggcaaagggc tttggaagat aatcgtcagc gccgaggtca
     7801 aggcctttta ttctctcttc aaccgcgccg cgggcagtga tgattatgaa tgcgctctct
     7861 gaactgatct ttctggcctc gctgataagg tctatgccgt tatagtcagg cagtcccagg
     7921 tcaagaagta taaagtcata aaggttgaca gcaatctttt cggatgcctc agctccggta
     7981 tgtgcaattt cgcaaaggaa attctccctt gtcaggtagc gtgcaatctc accggccaga
     8041 ctcttctcat cttcaacgat cagtatgttc attgttcaaa attaaaaaat cctgaagaaa
     8101 agggatacct gaaacaggaa tccgccattc cgtggcagct ccttcccggg ataaagtggg
     8161 atgatataac cgggttccag ctccagggta aaaaagtcat agctgaaggc tgcgggaagc
     8221 ccgagatcaa gttctatcag gccaaaagag ctggtgatgg ttgttccggt aggatggttc
     8281 ctgcctttcc tctccctctt ccatgtgcct gaacctatac ctgctcctgt tcctgttccc
     8341 tgattcatac ccgtcccggt cgcaccttgt gatgattcag tatggaatgt gcttcccgcc
     8401 agtatattga cgtaggggtc aatggagaaa aaggccttgt ctcgggcgaa agagcgggtc
     8461 tcaaagtatc ttgagaatct ggcctgcacg taggggagag tcccgtctgc catgaacagc
     8521 ccggcagaga gctgtgtgta gagtatcctc cagtcaaacc cgagagtcgc gtaagcatag
     8581 ctgaaattgc cgaagagcgt gtctctgagt ttttctgcca cattataccg tgagaaaccc
     8641 gtgtttatgt caaaccatga gttgaaagtg tgactgaagc tcaggtcaag gctgtagaag
     8701 gcgataaatg gcgagagccc gctgatggta taagttgttg ccgagagata cagcttctcc
     8761 ttaagcccgt atgcagccga aacatagcca aagctattgc cctgtgacat tgtggtaccg
     8821 agatacaaca gattattgcc atagccactg ccggcatata atgagctcag cctgtttcct
     8881 gcaacagagt cgtttgctcc ggcatggggt gtgaaagcac ttgccgggtt tgattgagca
     8941 gtgctgtgag gtgtcagcgt cagcaccgcc aggatgcaga caatcatata ttggagtagc
     9001 ctctctggtc tcatgataat cggatgatgt gaatttacct tctgccgggg cccctggcgc
     9061 ctgccggcct gcctgccccc tttggaatcc tcgagccgga aggcctctca atagatgccg
     9121 gacgggctcc ttttgccttg ctccagtcgg gccttgcacc ctggatcttc tttgccggga
     9181 cggcactggt gccccccggc tgtgaccccc ttttccctcc cggggctgtt tgtgactgac
     9241 tgccggaagc attggctctt tcccctgtct gtccgcccaa ccggacctgg ttttcaccat
     9301 ttgtctgatc cctggtgcgc aaccgatcct gtgtcttctg ttcctgggcc ttctgttcct
     9361 gtgccgtctg ttctgccctc ttctgttcct gtgccttctg ttcctgttct ttctgctcca
     9421 tctgttgcct ctgaagcatt acaggatcgc gaccggcctt cagttcctgt gcattcactg
     9481 ttattagtga agaaaccggt attattatca gaagcataac gatactctta ccgatctttt
     9541 ttagtttcat tgcggttctc cttatattga tacctgataa gtctgcttca ttatctgttt
     9601 aaaccgggct tgttttcaga ccatttattg aaatctcaaa tcgttttttc cttattcacg
     9661 gcaaagttaa gacttgtatt atgaagaaaa gatgaaatta ttcaattgag atattcactt
     9721 gatttaaact tgctgtccgg atgtatcagt cgcagacaga tttcctgatc gtaatatatt
     9781 cagattaacc ggtagtcttt ccggtttttt gcggtgtatt ataattgttt ttattttaag
     9841 gttgaaatag taatatcggt taatttccca ttcggaatat ttgctctaac gtctacaata
     9901 gaataaatat tagtttcaat cacctggaaa ataaatccct tagttacctg atactttcct
     9961 tttttcataa taaatctgat attacaatca gataaatcaa ctccgagttt attatttaaa
    10021 gtcgctttat ttttatctga agaaccgtta tttggttttt caaaatcaat tgtgagatta
    10081 agatctagtt cattatatga tttagtaggg tctttgcaaa atctcagtgg ttcagtatat
    10141 gtaaatttgt ctccatcgat ataaaatagc ctgaaaaatc ctagttcagc ttctgtatct
    10201 aacacaccac ttctgcatac agccccaggg cgtgtgctta atgtaggtgt aattccgaca
    10261 gattctacaa acggcttatg attgtgacca tgtagcagaa tgttgatttt gtacttatta
    10321 caaaatgacg tatcaattct gtcatgcctg tgctgtgcca taatgcgtaa taagcctgaa
    10381 ccattctgat tgaagaaaga agtaaaaaac ctaccctgaa ggccagacat tggagaagta
    10441 gccggtatat cagtaacagg atcacccata aaatcgtcag cagcaataac tcttgtttca
    10501 ccatatgaga agccataaat acgtttaccg cataaggagt tccactgagt aactttagct
    10561 ttatggtcat tctcgtctac accataaaag tcatgattgc ctgtgacaga gaatacaggt
    10621 gaattaaatc cattaattcc aagaaaatta ttcgctcctt caaaataatt tctccatttt
    10681 tcttctacca gtggccgtga tcctgcgtca gtaataagtg tgccacccca ccctgtagca
    10741 tcagcgttta ttcttgtgta attatgaata ttatctcctg ttacaagaat aaaatctgga
    10801 gaaattatat tggcaacttt caaaaaccca ttaagtagtt ccaattcttt tgcataacca
    10861 tttacaggag taccaaccca ttgacgtgat acatgtaaat cggatatatg aataaatgaa
    10921 tgttttggtt tgaattcctt taccaccttc acggatttaa gggacctttg tacttctccc
    10981 ccgcttttaa ttatcaaatc atacaattct tctggagttg agtctggaat agtgacccat
    11041 atctcattat tggtagaaga atgggaataa ggatcatatt caaatttgcc aattaaaaca
    11101 gaatctatat tcagaataat cttattataa ggtccttcta aaataactga atcaatcaat
    11161 attgatcttt cgttatcata tagtatgcta aaattatttc cacattttac tattgctgga
    11221 actgctcgcc atggatatat tatttttccg gcatttgctt gagctccaat taagccatac
    11281 aataatatag tccagaatat tattcttatg ttaatcctat tcataattta ttcatgctag
    11341 ctcattactg aagttgccag taaaaattaa ttttttaagt ttcaattctc aatgttaaga
    11401 tttgaagtaa ttcaaattta aaatgaaaat tgtttttaaa atagattctt taaagtatca
    11461 ttgttatact gactaattcc aacctgggtt ttgaggcata gattgtgaaa tatttgcatc
    11521 aatcactggt tgaggaatag gaaatatagt atctctttca gatattagac cacctccatt
    11581 attgttgtaa gccctcgtcc tttcaagcaa tttatgtgta cgaattaagg tatatctgcg
    11641 atgttcttcg agtattaatt cgcgtgatct ttcatcaaga ataaaatcaa tatttacatc
    11701 accagcagta atatcgcttg cattggatct cctcctaata atgttaattg tatttgctgc
    11761 ttcttcagat agacctaatt taaactgagc ttcagcctta aggagatagg tatctgctaa
    11821 tctgagataa atctgatcat tatattgata actgtcagat acattacttg ggtcagtacc
    11881 atttgtttta ttagaaaaag gccaattaag tcttgccctt gtttcataag taatatcatt
    11941 tgaccagtca agccagattg tatcaccata ttggtatcct ggaggtagtc gatctgcagg
    12001 ataaggagca ttatcttcag cattatttaa aataaaaaat tttctgattg cataatgtga
    12061 gcctctgtca tcctggggtt catataattc aagagcccat tttgaaaatg acattcgaga
    12121 tcttcccctg cctcctcttt cgtaggtgtc ttgtaaaggt ctgaccccac taatatttat
    12181 ttcagcatat ctgctgccgt gatgccgtct cattaaacac tttcctccac cgttagtaaa
    12241 aattgcaaac tggaaaaccc ataaagcttc agtgtttccc tgatttctgt tttcattacc
    12301 ttcgtaaaac atatccataa acggtacacc cggctggttg cttttcactc catatcggga
    12361 tgttattagt tggtaggcag gattattaat tacctgatct gcccaatata acgaactatc
    12421 tgggttattc ttaaccagat acatttcaga aagataatgt tgtacagctc cttttgttat
    12481 tctaccttga agtgaacctt caacaggtat atatttttct gcaaataaaa aatcagagat
    12541 tatctgtttt ctaacatcag ctgtaggtgt acgtgtccaa tttgttttta ttgtcgagcc
    12601 taatgattct tccaggctca atggaacatc gccccaacaa taagtcaaat gccggtaggc
    12661 ccagcctctt atagcttttg cttctgcaat tactctgttc ttattttctt cagcgggaaa
    12721 tcctcctcct gaccaatcaa tattatcctt cttttccgcc tgatttataa ttgtattagc
    12781 tgcatttatt atactataaa gccattcaaa aacaccaatg taatgagcat tttcaggatt
    12841 attcagatct ccccatcttt cacaaatcat agaaaaacca acatctctgt gattagtaac
    12901 cagattatca actccattca taaacatgtc cgcaatcaag tcattcgcac ctgtacggcc
    12961 ttctctttcc attctaacca taccatataa tccattgaga cctgtttcga atccggctaa
    13021 atttgtataa agagtttcag ttgttatcaa atgtggaggt ttttcactta accaactttc
    13081 gttacagctt acaaatatga atactgttgt tatggctatt aataaatgtt ttatactcat
    13141 gatagttatg attttcctag tcttactata aatatttaaa agcttgtatt taatcctata
    13201 acaaattccc tttgaagtgg agttgatgtc tggctgtcca gttcgggatc aagaccactc
    13261 cattttgtta aggttaataa attacgtcca gtaataaaag ctctcagatt tgttattttt
    13321 agcttttcta gaacgttttt agagaaatta taagaaaaag aaagatcttt taagcgtaaa
    13381 aaatctgctt tctcataata agctgcagtg atacccccca ttctggttgc atcataatgg
    13441 ttcataaccc atttatttgt tggatttgtt ggagtccacc agtctttttt tgttgtgctc
    13501 tgacgtgttt ctgcgaaagt tgcatctgcc atcaatgtgt ttctttttgt tactccatga
    13561 atgccgtgaa caaaaatgtc aagaccgaaa tttttatagg tgaatgaatt atttaaaccc
    13621 cataaaaact taggatccct ttggccaata attcttctat cagcagcagt gagtgtctta
    13681 tcaccatcgg catcccatag ttttacaaat ccaggttgtg atccccatga agcagcctct
    13741 tctgcttctt caagttgcca ggtacccaac catacataat caaaattaac tcgaattgga
    13801 taaccaataa accattcgtt tgcaacatca tcaatttctt tgcccttttc atccaatgtg
    13861 ccatataatg atactatttt attcttcagc aaggccagat tacctgacgt cgtccatgag
    13921 aaatcatttt taattatgtt tctcgagttc agggaaactt ctaaaccaat atttctagtc
    13981 tctccgatat tctgagtaat actggtaatt ccatggacag ctgaaatgga tcggtctaaa
    14041 agcaaatcgg ttgtattggt attataaata ttgatttctc cagagatcct gtttttatat
    14101 ataccaaaat caagtccaag atttatcgat cttgaggatt cccatccgag ttcatcttgc
    14161 cctaacttac taggtctata tcccgggaca gtagttttat tagaaaccat attgaactga
    14221 ctcaatcttg atattgattc ataagctgaa actgcctgat ttccgttata tcctatggaa
    14281 gctctgagtt ttaattcgtt aattatattt ttccaaggga aaaaactttc gtttgatata
    14341 ttccaaccta gagcaaacga agggaaaatc ccccatttgt gtttggttcc aaaaccagaa
    14401 taaccatctc ggcgacttgt cagtgtaacc aaatatcgac ttccatatga ataattaaat
    14461 ctgagcattt gagagatcag atagttctca ttgtaagaat aagccggaac aatcagttct
    14521 gcctgcgaag aagaatacca tgttaggaaa tcatgaggaa atcctgaggc gctcatacta
    14581 tttgaacttg ttttgttacc ttcaaaactg aatactccgg ttgcgaatat attgtgcaga
    14641 ccaaatgttc gtgaataaga taatacgttt tctataaccg tgctattaaa ttgattacgg
    14701 tcagtactgg cccgtcctcg tgcctccaat ccttctttag tatctcgccc agcgtaataa
    14761 cctttatcca gaaaacggaa tctaaagcct acattcaatc tataatttaa gcctggtata
    14821 aatggtacat caatagcagc ataattgttt aaaacaatct gatgtgattt atccacgtcc
    14881 ttatataaag taggtgcaag aggattttga aaataagtat cttcgggcca gggatatatt
    14941 gttagattac ctgcattgtc atacggtata ctaagtggac tcatttgcca tccggcctga
    15001 attgatggtg aacgcccact ctgatcgtca aatgaaaatt gagtacgggt gccaaaggaa
    15061 agccaattat tgaattttgt atcgatattg attctatttg taattctttt ataattatca
    15121 tttacaatta gaccttgtac atcgatgtaa cttccggcaa tatagtaact agtattttta
    15181 gaaccgcctg aaaatgaaag attatgttgc tggctatgac ctttttgtag aatcatgttc
    15241 atccagttca cccaatttcc agaatcataa acattttgtt cagtctgagt aagtagtatc
    15301 ccgggatgtc ttgtaagttt aaaatcatgg aattctctac cgtccataat gtcaggcata
    15361 ttaataaagt cctgtaccga atatttccct tcgtatgaga tcgagagttt tccttcctgt
    15421 ccttctttag tggtaatcaa tatgacccca ttagcccccc tggaaccata aatagctgca
    15481 gaagaagcat ctttcagaac ttcaattgac tgtacatcat tggtgttaat atcactaatc
    15541 tgccctccgt aaggaacacc atcaacaaca atcagaggat cgttactggc ttttattgaa
    15601 tttcggccac gaattaatat tacttcattt gatgcggctc cagcagatga ggtttggatc
    15661 attacccctg ggacggcacc ctgaattgcc tgggcaatat tcagatttgg aaccatttcc
    15721 aatctatctt ttggcaaaga agcaacagtt ccagttacat cgctcttctt ttgggttcca
    15781 taaccgacta cgattacttc ttcgagtcca agaatatttt cactgaggat gacatttatt
    15841 gttttttgat tacctacaat tacttcttct gaattgtaac cgacaaatga aataacgagt
    15901 atgtcagcag aactggaaac gtccaccgta aatctgccgt tcgcatcagt taagacccct
    15961 gttgttgttc ctttaacctg taccgtcgct cccggcagtg gggtattctg ggaatctgta
    16021 attactcctg atactgttat cttttgtgca tttaagaata aagatgcaat aagtaacagt
    16081 ataattaata agcccttttt catagatttt taaaatttat attatggtca gtagaatttt
    16141 aatgatttta aaaatattga attattggta aaataaaatc actttttcat gagtataact
    16201 taatttaaca taatttaact ctatgagatt aaaatatgta aacaaacctg ggtttccagg
    16261 aaaattgaac aaagttttga aagattgttt attagattta tgtgtaaagg gtcaattgta
    16321 attttgaatt gctcagtctt gcatttataa atgaaaatga cgtgaataat aatgttgcag
    16381 attctgaagg aggtcgaaaa tcccaggcat cacgtcaaaa aagttaaata tctagttttt
    16441 tatgattaat tcaattaaca tattcacgat aaaaaaatat tcatcctgtt atgcattttc
    16501 tgatgatcaa tacttgttgt atataaccag atgcataaag aaaatatgct tttcgaataa
    16561 tttatttcat ttgcgatcac catccaaaca aaactttacg ggctatcgca tcaaactatt
    16621 ttacgtctaa ccagaaatat aatgctgtga aagtggtgcg gacaaatata aatttaagta
    16681 tatactcacc caagtcaaag aagtataatt tatacatatt atccatgata gttacttatt
    16741 taacttccaa taagcttcga cttatactct ttaattattc ctatgcagct cttgtaagag
    16801 ggactcttat ctggaaacac agaatgataa taattctata accaatgcgc actaagaggg
    16861 tacattgatt ttcaaactta tatcttactt tttttctttc tatgctgtat ttcaaattgt
    16921 gtaaaccctt tgacggtcat ttaatgttcc gaaataaatt tgctcataaa ttcatgaatc
    16981 tttgaatatg agatacggac acatgcactt tttgattaaa aaacgctcat atattattaa
    17041 taatctgtta tttgattttc gctaagctca aatcccgaca tctgagtgtc accacaaaag
    17101 actcataagg aaaaaccatg gaaaactttc ctgatgagca tatcaaaaat attgataact
    17161 tgtattatgc ttttttcagc ttgacagaac aaccgattgc acgggtcttt gtgattgcga
    17221 ccggttctgc ccttagaagg gaattgactg catcctcaac atatttttta tcggcaagat
    17281 caggattttc agtattattg tctatggcac caacgtattc aaccttattg tttccgtcct
    17341 tttttgaaag gacataaaca tgaggtgtat tggtggctcc atacttattc agaacctcct
    17401 gtgtttcatc atacagatag ttgaatccga atcccttttc tgcggcaaac ttttgcattt
    17461 cctcgaatga atcgcccggg gatgtattta cgtcattgct gttaattgca attaccggat
    17521 aacctttgtc tgcatatttt ttatgcaggt cgataattct tgattcatat tgccggacta
    17581 ccggacaggt attgcatatg aatattacta taaagcccct ggcatcccgg aactgggaga
    17641 gagaaaccat tttcccgtca acatttttaa ggctgaaatc cgacgcatag tcacccggct
    17701 gatatggtcc ggcagcttcc gcagagacgg aagccattaa caatattgca aataaccagc
    17761 taaacttttt catatcatta cgagtttatt ttacataatt aatttaaaca ggtgtcgatg
    17821 aactttctta aaccggattc atctatttct ccggcatgaa aaaatcttgt cttccttgaa
    17881 ttgttgaaaa acaatgttgc aggaatatta ccctgccagg aaggatcgac tttttcaatc
    17941 cacgcgttgt aatcaagatc ggtcataaca cgtacatcca gagtgatcat gttctttttc
    18001 agaaagggga caagctgttt ctgaacctgg cccggaaaat caaggctgac cattataaag
    18061 ctgacctttt ccggatcata ttcctttgat attttctgga accccggcag ctccgctatg
    18121 cagggtgcac accaggtagc ccagaaattg actacataaa gtttgttttc cggtgctttc
    18181 agcagtttgt cgagatcagg gatgttgata tagcttattt cctgacttct cacttctctg
    18241 ccggttataa gcaagacaag aatgaaaaat aggatatagc ctggcctgga tttcacgcgt
    18301 ctgtcctttt aagggaaaaa caaaaaatca cctgaaaagt tcacacgaaa caggtataaa
    18361 gagaaattta agacatgtat gtatggcgga ggagagccat gttaataact tattcattca
    18421 tctggataat ttaaaatgtg tctgatactt tatatggtta tttttgttct gcaatcggtt
    18481 cttataagat taatagggaa tccggtgtga atccggagct atccccgtag ctgtaagttc
    18541 tatttcaagg ttcaatcatt acgccactgc tcccggagga gtgggaaggc gattgaaaca
    18601 gatcaagcca gaagacctgc cgatgcaaaa tttcgtagct ttcgggcgaa aagcaattga
    18661 atttctgcca tttgcaggca ttcttagaat cccccgcagg ttaccattac taatcttaaa
    18721 ttcatttaat atgaatggta aactattttt ttcacaaatc attaaacgta acggcgatgt
    18781 tgtacccttc aaccgcgaga aaattgaaaa tgctgttttt aaagcaacac gcgccatcgg
    18841 ccgtcctgac agggcaaaag cgaacagatt atcggcagat gttttggaaa aactgtcaaa
    18901 attagaaatc cgggaattac ctacagtgga acttgtacag gaccaggttg aaaggacatt
    18961 gtttgaagat ggtgattttc aactggcaaa agcttatatc ctgtacagaa aacaaaggga
    19021 gctggcaaga aatgcacagg aattattttc caacattgaa gttgttgacg attatcttgg
    19081 tatatctgac tggagggtca atgaaagtgc caattcagca tattcccttc aggggctgaa
    19141 ccagcatatc tccactacaa tcacttcaca gtactggctt gacaggatct atcctgagaa
    19201 tatctcctca gcccataagc aggggtattt ccatattcat gacctcgggt ttctgagtgt
    19261 ttattgtgtc ggctgggacc tgagggacct gcttctgtca ggattcaggg gtgttaacgg
    19321 gaagacgcag acaaaaccgg caagacattt caggactgcc ctcggacaaa ttgtaaattt
    19381 cttttatacg atgcagggtg aggctgcggg agcacaggct ttctcaaatt tcgatacctt
    19441 gcttgctcca tatatcagga aggataatct ggattataag caaattaagc aatgcatcca
    19501 ggaatttctc tttaatatga acatacctac aagagtaggt tttcagaccc cttttacaaa
    19561 tattacaatg gatcttcatg ttccggattt tcttaaacag gaacagattg tatggggtgg
    19621 tgagatacag gaagatgtat atggcagttt tcaaaacgaa attaccctgc tgaacaaggc
    19681 ctttgcagaa gtaatgctcg aaggtgatgc agcgggaagt ctgttctctt ttccaatacc
    19741 aacctataat attacaccgg attttgactg ggataattct gattacgatt caatttggga
    19801 aatgactgca aaatatggca taccctattt ttccaatttt gtgaattctg acatgaagcc
    19861 tgatgatgta agaagcatgt gttgccgtct tcgtcttgat aaaagagaat taatcaagcg
    19921 cggaggcgga ttatttgctt ccaatcctct tacgggttct gttggtgtgg taactattaa
    19981 tctttccaaa ctcggctacg aagcaatatc agagaataac ttttttgaaa agcttaaaaa
    20041 cctgatgatt cttgcacggg agagcctcga gataaaacgt aaaacaattg agaattatac
    20101 agaaaaaggc ctgtatccat attcaaaatt ttacttgcgt gatatttata aaagatttaa
    20161 ctgctattgg aaaaaccact tttcaactat cggaataaac ggaatgaacg aatgttgtct
    20221 taatttcctc ggaaaagata ttggtcatcc ggaaagcaag tcttttgcca tcagggtaat
    20281 ggattttatg agaaatgtgc tggaaggatt tcaggaagaa accggcaata tttataatct
    20341 cgaagctact ccggctgaaa gcactgcatt caggtttgca cggattgaca caatacagta
    20401 tccggatata atcactgcaa accagaaggc atataaggaa ggagctgcac cctactacac
    20461 aaactcaact catttgccgg tcggttattc tgatgatatt tatgaagtat tatcgttgca
    20521 ggatgatctt caggtgaaat ataccggtgg tacggttctt catgttttta ccggggatac
    20581 aaatatgccg gccatgtctg caaaaaacct gatcaggaaa ataacaaagg cttttcacct
    20641 tccttatata accatctctc cgactttcag catctgtccc tcacatggct atattccggg
    20701 agaacacttc agctgccctg agtgtaattc ggaaactgac gtatatagca ggattgtcgg
    20761 gtatatgagg ccggttaacc aatggaacaa agggaagaag caggaattta aggacaggaa
    20821 actgttcagg ccggacaccg gtaataagga aatggaggaa gatcagggaa tggaggaaaa
    20881 tcagggaatc cctgaaaagg cgtctctgat tccggaatta aataaatccc gctaatgcgt
    20941 atcggcgggt ttcttaaaca tagtctgatt gactgggagg gaatgattac tgctgtgatt
    21001 tttacccggg gttgcaattt ccggtgtgga tactgtcata atccctccct ggtccttccc
    21061 ggtttgttca atgaaacaga agatatagaa agtgaagaaa tactttctta cctgaatatc
    21121 cgcaaacaat ggatagatgg tgtggttatt tcaggaggag aaccgactgt tcatcccgat
    21181 ctccccgagt ttattaaact tgtcaggaat gccggctaca ggatcaaact tgacaccaat
    21241 ggcagtaacc cggtaatgct gaaggagctt gtttctgccg gacttgtcga ttttgttgcc
    21301 atggatatca agacattgct taaatcttcc gcgtattatg agattaccgg atcctccagt
    21361 ctccgcctta ttcagcgtat ttcagaatca gtcagtattt taagaacttc cggagtacag
    21421 tatcagttca ggactacact ggttcccggg tttcatacag aagaaattgt tgagcaatta
    21481 aaaaaagaat tcaaaggtga ccccttggtc ctgcagcaat tcagggaggg agtcaccatt
    21541 gaatctctta atgaccgcca gtcgtttcct aatagctgaa aaccactgaa cagggaaact
    21601 ttgccgtttc gaattccaat tgcttcaccc ctgcggtatt ataaacaaca gctttggaat
    21661 tgactgcatc cagacagaca atattgccgc tttcaggatc cacgttcagt ccataaaact
    21721 cctgatcagc cactttggtg tccggtaatt cagttgcagt taccggcatg gaataaagag
    21781 catcgttaag aaaataaatc gtgctgccat cttttcctgc cgcgatatta ttgattcccg
    21841 gggcgctcat ggtgctgacc gggaaagaac tcaccgtatt ggcagatatg ttgatcctgc
    21901 ttatcccggc atccgtatag cttacttccg gccagtttgt ataatcagca atacctttgc
    21961 agtaggccca tacattgttg tctttatcgg ataccagatc cacgggaacc gaagctacgg
    22021 tataagtgcc ggttacagag aaagtaacag ggtcgatctg aataaccgta ttgccgtcat
    22081 tgttccatcc tcctgaaata gccgcatata cttttgaacc ggcagctatg agcttttcgg
    22141 gtcctgtgct gagcgcaagg gatccgctta tctccagggt cgacaggtta attttgtaaa
    22201 taaaattatc ggatgatccg tttccgtttg atacatacag tgtgttctca tcagcacgga
    22261 caatactgcg ggggtaggaa aagccatcaa tggttttgac cagtttgaaa tccttcatgt
    22321 ttaccacagt tactttctgc gaattgttaa caacgataaa tccgagagta tctgctatta
    22381 caaatgactg gacgacatcg ccgagttcca ttccgttgac agaataaaaa agattttttg
    22441 tagtctgccc ggtctttgaa tcatagaaac taatatcacc gtttgcctgt ccaaagaccc
    22501 cttcacacac aacaaacata cccttcaggt tattaatagt aataggcggg tcatcaacgg
    22561 ttttttcaca tgatgacacc agcaggagca ctgtaatcag ggcaaaggcg ggaaacaggg
    22621 ttttcaatct gatttttttc ataatcattg ttttagatag aattaaagga tattaaaagg
    22681 tatagtttat tttgactgac cagtaacggc ccggcatcgg gtatagtctg attagctcat
    22741 aggtggtatt tgtcagatta ttaacactaa gatgaatacc cagatgtccg tttgcgacag
    22801 ggattttcac tcctgttttc atatccagaa gctggtaagg cttcagcaat gaattgtcat
    22861 cataaaaacg gtctgcactg aaatggtgat caatagccat atcaaatatt cccgctgaca
    22921 aactgttttc ccatgaagtg ataatccggg gtgaatacag cattttttca tgctcacctg
    22981 acacatacgg ctgtacatcc ggcctgttaa gcattacatt aagcgccgaa cggtatttta
    23041 attttctgtg tttcacatcc agaatcagtt tgcaatccat tccggcagaa tatactctct
    23101 ggtaattcat ggcagtccag tacatcccgt cgggccgcca tgcgatcata ttgtttattt
    23161 ttgaccagta aatgcccgca tcggctgaaa gaacggcttt ttctttttta aagaccgtta
    23221 ctgaggagcc tgcttcgacc gaatatccgg tttcaggctt taaatcagga ttacctcccg
    23281 gcatccagaa aagatcatta aatgtgggtt tgcggaattt ctgtgaaaca ttgagtctca
    23341 gtttccactt gtcagttatc atttcccgcg acatgccgaa ggaagacaat acacctgaat
    23401 gaaaattaga attccactct tttcttaatc ccgcctgggt tgagaaccgg tccaagtcat
    23461 attttattcc ggcaaaagcc gtcagtcctc tttcctcttt cctgtgcccg taggctgaca
    23521 catccgcatt gataaaagta ccgatcaggc cggcatctac cgaaatgttc cggccggcat
    23581 gatacctgaa atttacatca ccatagtact gaccggatct gatccttgaa tctatgctgt
    23641 atgattccgc ctgttcggat gctttcatag tgtatctttc atgatcctta aacacagccg
    23701 tctttgcctc caggctccag cggtgacccg attttttcca ggcggtaaag aatttcagcg
    23761 ttgaatctct ctgatgctcc caggataccg atcctatccg ggaagggata ttaaaggcct
    23821 ttacctggta ccagaatcct gcctcaatgg ttgatgacgg ggaaaggcgg aaaatgaact
    23881 gctgaacggt acctgtactg tgccactgcc cgtctgtttg ctttctgtca gtctgtctaa
    23941 tgtagtcata gtagctgaac tcattgcctg aaagagcacc ccatgcatcg gctttccatg
    24001 ccagcctgtt attcccggtg gtaaatgata agctgccgtt aattgtttcg atggactcat
    24061 agcttatatt aacagatccc ttgagtgcct tcaccgtttt caggttatta tccagattga
    24121 cggccccgcc gaatgtgccg cttccataca gagctccggg agctccgtaa acaacagata
    24181 tattatcgaa tccggaaaca gggataaatg aaaagtcact cgaacccagg gtgaccgagt
    24241 tgatgggaaa accgttccag ttgacctgaa catgacttgt tgatgtaccc ctcagggaaa
    24301 tgttgctcag agcaccgcca acaccataac tcttgacatt cagggctgtc ttgctgataa
    24361 ggaaacgtcc gagtgattct cctgcaaaag ggctgagctc ttcagaagta aattcatctg
    24421 ttttaatatc acttctgaac tgttgaaacc tggaagctct tacaaccaca tcgggtatct
    24481 gaattatggt atcggcaatg gattgtgaaa acataaccgt actataagtt attattaaca
    24541 atattgccgg caggactgac ctgaaataca acagagcttt tctaatcatt tccgccatat
    24601 aattctttgc gttaacattg cgacaagtgc atcattaatg gcgatgcagt tcacatgcaa
    24661 aaatcttcgt agaaataatt aaaaggcggg ggcggaaaca tggcagattt ccttctttgc
    24721 ctttcacccg aaagctacga agcatgttac aggcaggtct tctggcttgt cctgttccgg
    24781 ctgccttccc atctcacctg gagtttaaca gtggcagggt atgccggaac gcttgcagga
    24841 cttacagctg cgggtacagc tcctgttttt cacaggattc cctcttcgcc cgggtttgta
    24901 tctgactgac ccgggaacct gtaatcgcag agcaaatata tctttttttt ctgaaagaaa
    24961 ttaattccgc ttattatacg acttttttcc cgcaggtaaa cccggtccgg aagatcatac
    25021 cggcattagg gatttcctgt ccggatgccg gtgatctaat ttcctggtat aaagagtttg
    25081 atgctgctgt ctttgccatt ttcattgacc agtaaaacat atattccggt gataagacca
    25141 ttgacattta tcctgttggt cccagtctct ccaatttttc ttatccatat tgcattcccc
    25201 tttaagtcgt atagtaaagc aacagctttt tcagtaattc cgttaattac aatctcgtct
    25261 ctttccatcc atgcggtaat ctttttcagc tcgcctgaat ttgtgtctgt gatgcctgtt
    25321 atgagtcctt cacagctttc gtttgcaaag gaatcacctt ctatagtcca tcccagtgtt
    25381 tctgtcagat aagtccttgc tgcttcggca gcagtaccgt attgcatgtc tgctgctccc
    25441 agtaccctgt ttaagggagt atttggattg gcagaccagc ctgccagtgt tgcggaatag
    25501 tcaatacagc tcatgccgct gccgtcaaac atatattcca gcataactcc ggggtttaaa
    25561 gtccatgaac cgatgttccg gctgaaaccg gaagcttcca caaacatatc cctcatatcg
    25621 ttaacagaag atgtattcca tgaactgatg tcgctgttga aaccggcgga tccggcaaac
    25681 atagcattca tactggtaac agcacccgtg ttccagctgc caatttcccc gttaaattcg
    25741 attgttccct ggaacatacc gctcatatcg ttaacagaag atgtattcca tgagctgatg
    25801 ttccggttga agctgtaggc ccaggcaaac atctcactca tactgcttac agcagccgtg
    25861 ttccagttgc caatatcccg gtcaaaggca tttgcgttcc tgaacatacc actcatatca
    25921 ttagcagatg atgtattcca tgaaccgatg tcacggttga aactggtggc cccggcaaac
    25981 atatagctca tattggtaac ggaagccgta ttccaggtat tgatattttc aggcgaatcc
    26041 agggcagtgc aatactggaa catcagtgcc atatttgtaa ggcctgacag gtccggcaca
    26101 tctacggcag ttatctgaag attcgcacaa ccactaaatg ccaatgccat acttgtccag
    26161 gctgtgctgc cccagttttc gaccagtgtc agcctgttac ggtcaatacc accgtcaata
    26221 aagaatcttt ccaggttttc aggctgaata ttcagacgaa ttatggcacc tgcaggcagg
    26281 ttacttatgg ttgcttccgg tccgctgaat gttccggaac ctgaataagc ggcaggtatg
    26341 gtttcccagg tataggcaac tgtgcctctc gttgccacgt tgaaagttat ttccgtattt
    26401 gccccgccgg gaattgcaag attccagaca gttacaaact gatt
//
LOCUS       DAOSPX010000008        25902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2085_length_25902_cov_4.863543, whole genome shotgun sequence.
ACCESSION   DAOSPX010000008 DAOSPX010000000
VERSION     DAOSPX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25902
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_25902_cov_4.863543"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLZ75_00910"
     CDS             <1..267
                     /locus_tag="PLZ75_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70989.1"
                     /translation="NSRDKLDLTRLKTWCILINTTDTEFEAWVRKATEENLWLILLYH
                     EIEGTNGKDDATLEMFTRHMRIINEYGIEVVTMSEALEKLADYL"
     gene            complement(396..1844)
                     /locus_tag="PLZ75_00915"
     CDS             complement(396..1844)
                     /locus_tag="PLZ75_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS70990.1"
                     /translation="MKRLELIPAEKITDRLRYENPWWNTRQVSETYSSMSRRLYFELF
                     YPYVKERSVRRALVLMGPRRVGKTVMLYHSIHQLLGDDVDPRKVIFVGIDNPIYLHLG
                     LEDILELCKNALNIEDMADCYIFFDEIQYLKDWERHLKVLVDSYPETKFIVSGSAAAA
                     LKWHSTESGAGRFTDFMLPPLTFHEYIHLKKLDHLIYDGEIIYGPDKISYKLAYDIKE
                     LNREFVKYLNFGGYPEVVLSEKIQSNMGRYVKSDIVDKVLLRDLPSLYGIRDVQELYR
                     FFTYIAYHTGKEFSYETMSRQSGIQKEMLKKYLEYLEAAFLVKVMHKVNEHAGRFKRI
                     TGFKVYLTNSSLRTALFSPITETDSDMGNMVETAVLSQWMHRETLDLKYARWRSGRTE
                     GEVDLIQLSEKDFKPLWAVEIKWSNRYFTKPGDLKSLVCFCGRNNIEKALVTTIDAQA
                     VKELDNLKLTFIPSSVYTYNIGVNTLTKKSGI"
     gene            2327..3277
                     /locus_tag="PLZ75_00920"
     CDS             2327..3277
                     /locus_tag="PLZ75_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70991.1"
                     /translation="MELLNLYNLKMTVFIRFKDYSKKEYRALTDDVLTMHLQGIETVG
                     IYPLLEDNTSYFIAADFDDRNWAESILKLHSVCSKHELPSYVERSRSGNGGHLWIFFE
                     ESLPAAKTRKLMYELLREATIISPFAKEPSFDRLFPNQDYHSGKGPGNLIALPLNGKA
                     LKTFNSAFLDPADLQPYDNQFEIIKNFRKLSVYQFNTIYQEIFNEKVDVFFQSGFDRS
                     ASPIIEIQIDGQIYLKRLHLNRKLTEFIREKLNFLNSDYLVKKNMGKSTFTLEKYFRL
                     IEETPDEIILPRGFVANLIFFCRKENYSCNDPVSFEKQRD"
     gene            3243..4019
                     /locus_tag="PLZ75_00925"
     CDS             3243..4019
                     /locus_tag="PLZ75_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOS70992.1"
                     /translation="MIQSLSRSKEIEKISGRFGTIIVDECHHIPAKSFREAIVNFNSY
                     YLYGLTATPKRKNNDEKLIYFYIGSILYNADQVERIRQKQIKVEINIKDTNLYVPFNY
                     KIDKYETISKILIHDTNRNTLILDDIEKNSSRFKTFLILTERKAHVDILNLYLKEKYE
                     TITIHGEDSESSRKSKIEQLKQGHFKIVISTGQYFGEGIDLNNLECLMIAYPFAFEGK
                     LVQYIGRIQHSGNPPVIFDYRDSKIDYFEKMFRQRNRYYK"
     gene            complement(4411..5532)
                     /locus_tag="PLZ75_00930"
     CDS             complement(4411..5532)
                     /locus_tag="PLZ75_00930"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOS70993.1"
                     /translation="MRPDIHLQKELLSPPGDTIQETIDALGMSQAELAERIGRSKEKL
                     NGLINGSEPLTLKTAILLERVLDIPAAFWLEREKEYRLDLAKIEEQEFLTGCIEWTEK
                     FPVKELKKDTWLPDTKDKAAISGALLKFFSVASPEQWNNIYLNESVTASFKISLANTQ
                     SPHSISAWLRIGELKTKQLKLPEYNKKAFSGALNEIKKIVFDHPHDSLIQLQEKCFRC
                     GVAVLYTACLPKAPVSGAVWWRGNNPIIQLSGRYKTNDSFWFAFYHEAGHILKHGKKE
                     IFLEDVNGSPMDEEKESEADEFAQKKLFPEAAFETLKKIIDLKVSDIIEFADKYETHP
                     AIIVGQLQHFNIISHSSFNHLKVPVNIPQDNLLRNKTNE"
     gene            complement(5544..5876)
                     /locus_tag="PLZ75_00935"
     CDS             complement(5544..5876)
                     /locus_tag="PLZ75_00935"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOS70994.1"
                     /translation="MKITYKTNKLERQLTVAKEMQKAFGTMAKKVNQRMKELRASSNL
                     EVMKTIPAANCHELKGDRQGEFAVNISGNYRIIFEPDHDPLPLKEDNSIDCIRIIDIR
                     ILGTEDYH"
     gene            complement(6366..6572)
                     /locus_tag="PLZ75_00940"
     CDS             complement(6366..6572)
                     /locus_tag="PLZ75_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70995.1"
                     /translation="MTVRNKFSDTFVKTGIIDVKFGKLLSHLADYRQKGDYGDLYDFD
                     DKIVLPLVDQVKEFISEIKILIEK"
     gene            complement(6738..7607)
                     /locus_tag="PLZ75_00945"
     CDS             complement(6738..7607)
                     /locus_tag="PLZ75_00945"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HOS70996.1"
                     /translation="MKKNILSMFLIILAPIIAGQEIGKISGTEKNAINKIGAIPVESI
                     GNIAGIRVQHYDKNCKEILLNNPSSPDGIYTIDPDGQGGLEPFNCYCDMTTDGGGWTM
                     VGYYKRPTTVNAPDDLDNRDYAYFMKARNNEAYGRPEFIANPDSEGAWTDWRILSGVT
                     WPVEFAVILDQQPWSTGWDDYRIKVIYRVKNRDIMPNYGTEQDLTTGNNLWYKFNPTG
                     NWIDVGSSSFALTYAWYPYAASNKYLILFHESNFKYVDGREPTNYQYGAYYGGGVSGG
                     NDTWYHSARMLIR"
     gene            complement(7706..9232)
                     /locus_tag="PLZ75_00950"
     CDS             complement(7706..9232)
                     /locus_tag="PLZ75_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS70997.1"
                     /translation="MKKLFAILIAILLTAAIWAQIPEKLSYQAVIRNSSDALVINRQV
                     GMQISILQGSASGTAVYTETQAPSTNANGLVSIEIGGGTVVSGNFSAIDWANGPYFIK
                     TETDPNGGTGYTITGTSQLLSVPYALYAKTSGDAFSGDYNDLVNVPTMVTKPENIYQV
                     RLMSADEDYIDFGTFEGFTNNSDWSVIERVMMPAGTGSGGGGWHFFRGKAWQDKEGDI
                     AINIRTIGLHAWIRKDGWRDIIYNTSLEEMTWYVLCLQYDATSKVLELYLDGQLVGQL
                     TGVFPQDDSGNTNKMLWGGQDVDPSRGEGDLYAEASIIYASQHWLQRKLTPEEIRGYD
                     GTFKPEPALFFAAEINAASVTDDTGNGHDGTNGNTPEFVSGLVVQGSETNEPVFISGV
                     TNYGEIVTDIMAAGTALFMNSAGKYEKADAGSVSTMPCVALALETGTGSKQVLLQGYI
                     RNDSWNFIPGAPVYISPTAGTLTQTIPSETGQQVQIVGYASKANTVYFNPNLMLIEIK
                     "
     gene            complement(9239..9748)
                     /locus_tag="PLZ75_00955"
     CDS             complement(9239..9748)
                     /locus_tag="PLZ75_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS70998.1"
                     /translation="MKINYKRIMKVFNITLVILCLICFEFTIQAQNGIPATGGDASGS
                     GGSASYSVGLPFYTVYAGANGSVVHGVQQPYEISMVTAIEEIIGIDLLFLAYPNPVRD
                     HLTLDVNDSDIANLHFHLYDANGRLLQSRKIQGQQTTIAVGNLIPATYFIKVTSGKKE
                     LKTFKIIKN"
     gene            complement(10164..11288)
                     /locus_tag="PLZ75_00960"
     CDS             complement(10164..11288)
                     /locus_tag="PLZ75_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOS70999.1"
                     /translation="MKQYKSGQYISQGCYKSFQPTLINKQLHVDNMEVLQLLSQADRE
                     LGRLDMFSKYIPDVDLFISMHVLKEATQSSRIEGTQTNMEEALLDREDIPLDKRDDWE
                     EVQNYAKAMEWAINELDKLPFSSRLIRETHKILLQGVRGERKQPGEFRNSQNWIGGAT
                     INDAIFIPPVHTTVPELMSDIEKFLYNEEIFIPELLKIGLVHYQFETIHPFLDGNGRI
                     GRLLIPLYLVSKGILKKPILYLSDFFEKNRKLYYENLMIVRDKNNLSQWFKFFLIGII
                     KTAKNGITTFDNILQLQKTIESSIQKLGSRAVNAKKIVDYLYNRPIINAEKVSEIAGI
                     SMPSAYKLIVDLEKLDILKEITGSKRGKVYVFDNYLRLFR"
     gene            11441..12626
                     /locus_tag="PLZ75_00965"
                     /pseudo
     CDS             11441..12626
                     /locus_tag="PLZ75_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611811.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(13032..14066)
                     /locus_tag="PLZ75_00970"
     CDS             complement(13032..14066)
                     /locus_tag="PLZ75_00970"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS71000.1"
                     /translation="MKKLTQPALLLGISLIISHATSNAQIKPEDVSVRKIWSNEYHNA
                     FTSLIWFKGSFYCSFRQGSDHAGGVDGVVRIIKSKDGINWTSVAVLEKKGYDLRDPKL
                     SVTPDGRILVNMGGSVYKDRKLMSRLSHVSFSNSSGKKFSDPQPIGISANAKTNFDWL
                     WRVSWYNKIGYGVAYSTGEEKEDQEWSVCLLKTTDGINYDLVTRLSLDGRPNETTVRI
                     MPDGEMLMMVRRESGTGFWGRSKPPYKEWSWTDTGIRFGGPDFTALNDELFIAGTRIY
                     EKPAITGLFLVNREGQFRNLLRLPSGGDNSYPGFVVGRKKIFVSYYSSHEGNSSIYLA
                     EIPLSLIEGL"
     gene            complement(14209..15798)
                     /locus_tag="PLZ75_00975"
     CDS             complement(14209..15798)
                     /locus_tag="PLZ75_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOS71001.1"
                     /translation="MVLSCTGHTDTISIKIENPVLPVLTGKDANPVLKIKMIRQQPAD
                     YSLQKVVISLEGTTDLNDIEHVSLFKSDGEGRFTTGNLIGKVQSPASVVTFKDNFNIT
                     EDTLTFWLSVTLKESMDLSHRIKVQCKSITTDIGKVPVPSDDTQSLRVGVAVRQHMKD
                     DVHTSRIPGLATSKDGTLLAIYDARYESARDLQGHIDIGLNRSTDGGRTWQPMQIVLD
                     MKEWGGLPEKYNGVSDACILVDDNTGDIYIAGLWMHGVLDPETGKWVKGMTKDSIRWI
                     HQWQAKGSQPGFGVKETSQFLITKSTDDGLTWSEPVNITSVKKKEWWLFAPAPGHGIT
                     LSDGTLVFPTQGRDETGKPFSNITWSKDGGITWTTSNPAHKNTTECMAVQLSDGSIML
                     NMRDNRNRGNDEVNGRTIATTTDLGETWTEHPTSRKALIEPTCMASLHKHVYHEKGVE
                     KSVLLFANPASTSNRDHITLKVSKDDGNTWPEDKHILLDEYSGRGYSCITSVNDSTIG
                     ILFESSQADIVFQTVSLKEIR"
     gene            complement(16088..16921)
                     /locus_tag="PLZ75_00980"
     CDS             complement(16088..16921)
                     /locus_tag="PLZ75_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOS71002.1"
                     /translation="MKPQLQYGCSEKDIVEYTCRKTAERIEINGDLTKPVWLNAARSG
                     RFVDMVTGDPGLYDTRAAALWNEEYLYIAFWAEEPFVTATLTEKDSIIFLENDLEVFI
                     DGGDCYYELEINALNTIYEVFFIWRDAYEKGGRFDVPEFDVFKKTSMTFGGDYDRTGK
                     YFWWGKHPRGLRWAFLDYDMPGLETAVKIDGTINDNSDTDKGWAAEIAIPWKSLKWLA
                     NGRALPPEEGDVWRIFLGRFQKMSPSGQEIIPHPAWAMNRHGVYDTHIPECFSRFRFV
                     T"
     gene            complement(16960..19554)
                     /locus_tag="PLZ75_00985"
     CDS             complement(16960..19554)
                     /locus_tag="PLZ75_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS71003.1"
                     /translation="MSLQLSSQAVTYLLHDNWKAKRASDVLIDGCELTGSELMPEGWM
                     EAVVPGTVLTTLLHNKLVPDPFYGLNNNLIPDIYDTGRDEYTYWFYNEFEIPDLKEGQ
                     QAWLSFRGINYFADIFLNGHRVNASTHRGMFLREKYLITKYISKDGPNRLAVLVAPPD
                     PPGNAAAGQGGDGTIGRNVAMQFTAGWDWICPVRDRNTGIWDQVAVEITGAADLRNPY
                     VETRVPGTRMPGEKQAPAFVRTSVEIKNPTSRVINGILRLEFDRYKVSINAVLEPREE
                     KVFSFPEIKVEEPRLWWPNGIGDQPLYNMKIDYSDRNGTKFDSETLTFGIRETSNYFD
                     SVITAQVFCVNGQKIFIKGGNWIASDALLRLSKERYDAEIRLHAGMNMNMIRVWGGGL
                     TERPEFYEACDKYGILVWQDIWITGDCNGEWDDPLKKESQARRKAYPDDHDLFLESLT
                     DQIKMLRNHPSLYLWCGGNETYPPADILQACEKDIFPAYDPSRFFLDMSTSPKLMTNI
                     IGGVGDGPYGIREPEEIFTVRSFPFNPETGSIGIPNYDGLIKFIPENELKPPQSPRAS
                     GSWAYHKYLPLMDFPDRYGRVKDVRDFCMKAQLVSYEQYRALQEAFNHKMWDWYTGML
                     VWKNQNPWTSLRGFFYDYYLDYTGAYFGYRHAAAPVHIQLNLSDSVVCVLNQTSKDLN
                     NITASVTLYDLHGRIISESGKTISLKANDLVLLNKVVLPETSTEELYFLKLILRDKEK
                     IIDENLYWLSNKPKSYAKLNELTEVTLKTDIVKSTDNKYIINVTNPADETAFFIRLKV
                     NDPDGKLVLPVFLTENYFTLLPGESRAVELDIPSLLANPPSGIKQKAGELRLITEGWN
                     IKAQEKTF"
     gene            19765..20919
                     /locus_tag="PLZ75_00990"
     CDS             19765..20919
                     /locus_tag="PLZ75_00990"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOS71004.1"
                     /translation="MKKTLFNNYLSGLRFALVSAIVLPMLVSCATSPDVLEDPHSIIP
                     PKPDPAGYVCYRTTGNITTDGILNESDWEAAPWTDYFVDIEGDLKPAPHYKTRLKMLW
                     DSEYLYIAAEMEEPHIWATLRQRDTVIFFDNDFEVFIDPDGDTHNYYELEVNAFGTSW
                     DLLLIKPYRDGGPAVTCWDIKGLKVGTRVDGSINNPRDADRGWNIEIAIPFSVLRECN
                     TGPALPGQGDQWRINFSRVEWRTVIEDGRYRKETDPSTGRPWPEYNWVWTPQGRINMH
                     MPEMWGYLQFSGIKAGEGTEVFVPDRDLNEKWALRMVYYLQNEYFKKFGTYTSDIRML
                     GLGRDDFEGVNTLPVIRATGTTFECYFSNENGGRGWTIYHDGKIVNLSGK"
     gene            complement(20960..21157)
                     /locus_tag="PLZ75_00995"
     CDS             complement(20960..21157)
                     /locus_tag="PLZ75_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71005.1"
                     /translation="MSVRTQIKFLFTTPGSDEWDPVMSIRLHEPQIMPQGGIKRMLVI
                     QAGPREPPVKHRGGDKWGLVI"
     gene            21200..22477
                     /locus_tag="PLZ75_01000"
     CDS             21200..22477
                     /locus_tag="PLZ75_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS71006.1"
                     /translation="MKTNWLLLSFVILLWFCAGNDSKCQSIGLVDVRYAEQVITVGGP
                     GADIPGYTSQAVQIALDALKSRGGGTVKLNPGIYEMTGPLRLSDNMTLRGSGESTVLR
                     KCDGFKTSFVIDADWGMLKAVVKDASGFKKGMGIQIFDDSHKSAWDVTTAVITDIQGN
                     TIYFDNGTVNDYVASLNGTISNSFSLIEAVEAENVKISDLVIEGNGAANEYINGCRGG
                     GVYMHKSRNCLVENVRVNEFNGDSFSWQVTENISVKGCEASHGRGLGFHPGAGSDHTV
                     IEDCISHHNRGDGIFLCWRVQNGIFRNNRVYANSDNGFSIGHKDTDNIFENNHVYENG
                     RHGVYFRNENEQNGGHRNTFRNNIIENNGMAGESAGFRIDGETRDISIISNTIRSTGK
                     GKQTVAISAGRKTSGIISENNSISGSREIVYEK"
     gene            complement(22516..23547)
                     /locus_tag="PLZ75_01005"
     CDS             complement(22516..23547)
                     /locus_tag="PLZ75_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS71007.1"
                     /translation="MIRSLIYSTFSGMIPGKFSGISFSAGISFSAGGQEKNFEPSYLK
                     LHRSGELKRRGEKLWAMMESCELCPRMCRVNKLKGEKGFCQADSTLEISSAHPHFGEE
                     RPLVGKGGSGTIFLSNCSLRCVFCINYSISQEGEGTKRSIDQFAGMMLSLQKMGCPNI
                     NVVTPTHYSPHILLALDRAASKGLKVPLVYNTCGWERTEILKILDGVVDIYLPDFKYY
                     DGQKAARYSSGAREYPEMTKQALLEMHRQVGVAKPAAGGLMQRGLMIRHLVMPNKVSD
                     SEKIIDWIAENLPRDTYLNIMSQYSPTYKAFDYPEISRKITREEYFSAVNHAKAAGLT
                     NLEIQGYRY"
     gene            complement(23878..>25902)
                     /locus_tag="PLZ75_01010"
     CDS             complement(23878..>25902)
                     /locus_tag="PLZ75_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS71008.1"
                     /translation="KMKKIPEYLLLATLAVLTCSCSQKQAAPDNSGEMANNIRNYLVK
                     AAKDITDNSGSTVLSASEWEKVRPERHSEFIEMISLQDMPLTGERPPLNVRITGTVRR
                     DGYRIEKLYYESLPGLYVPANLYIPDNVSEPVPGIIYLCGHSGTQKVHYQAHPAKFAR
                     LGFVCLIVETIQYGEVRGEHHGCYARGWFNWYSRGYTPAGVEVWNAIRGLDLLSERPE
                     VDEQNLGATGISGGGAISWFLAAADTRVKASAPVCGASTMEAQITTRTIDDHCDCMMA
                     INSGLIDFQDIGALIAPRPLLIGQSDRDGLNQIESVRRIYRDLRRIYELHNAAADIEY
                     IETPGGHAYHKNSREKIFSFFARHLMGKNLSPEEAGDIDTSPGNMLSEDELRVYTAGA
                     PADDRTTTIQDSFVKLAGDPEITSEDDLIAHRKAVTDFLRKKSFSAFPQNPPPFDSVK
                     VFRTLDRASYGSDIYSFVSEEGWRLKVDIRWRNEASQKKPLMIVLRNPGESRWESEGF
                     IGDPGKDWNIVYFEVRGAGEYGWDPNLQWHIRRASAWLGRTPASMQVYDLMRCIGFCR
                     TLGGVNADSIGIAARDAMAVVALYAALLDGNCKSIILKDPPASQDVASSPDGRGAAIE
                     ILNCLRITDVYQLPAFLSPAEIIFVGDIPEAYRWSDNVLRKLGKKPLRKL"
BASE COUNT         7213 a         5604 c         5314 g         7771 t
ORIGIN      
        1 aactcaagag acaaactgga tttaacccgc ctgaaaacgt ggtgcattct tatcaatacc
       61 actgataccg agtttgaggc atgggtacga aaagccacag aagaaaacct gtggctcatt
      121 cttttgtacc atgagattga aggtaccaac ggcaaggacg atgcaacgct ggaaatgttc
      181 accaggcaca tgagaataat taatgaatat ggtattgagg tggttacgat gtcagaagcg
      241 cttgagaaac tggcggatta cctgtgacag tcaagacttg atctggcatc agtgatatta
      301 taattagcca tttaccgctt cagccaagta ttggctaatt catataattc ttatacaaat
      361 ctatttctat cctctttcaa taaaatgcct gccaatcaga taccagactt ttttgttaat
      421 gtgttaacac caatattgta ggtatacaca gaagaaggga taaaggttag tttcagatta
      481 tccaactctt ttacggcctg tgcatcaatt gttgttacca atgctttttc aatattgttc
      541 ctgccacaaa aacagaccag acttttcaag tctcccggtt tggtaaaata ccggttactc
      601 cattttattt cgacggccca taaaggttta aaatcttttt cactcaattg aatcagatct
      661 acctcacctt cggttcttcc tgagcgccag cgggcatatt ttaggtccag cgtttcacgg
      721 tgcatccatt gtgaaagcac agctgtttcc accatattac ccatatcgct atcggtttct
      781 gttattggtg aaaaaagagc tgttcgcagt gatgagtttg tcagatatac cttaaaacct
      841 gtaatacgct taaaccttcc ggcatgctca ttcactttgt gcattacttt cactaaaaag
      901 gcagcttcaa gatattcaag atactttttc aacatctctt tttgtattcc gctttgtcgt
      961 gacatcgttt cgtatgaaaa ctcctttccc gtatgatagg caatgtatgt gaaaaaccga
     1021 tacagctcct gtacatctct gattccgtac aagctgggca gatcccgaag taaaacctta
     1081 tcaacaatat cacttttaac ataacgaccc atgttgctct gaattttttc agacaaaaca
     1141 acttccggat acccgccaaa attaaggtac ttaacgaatt ctctgtttaa ttcttttata
     1201 tcataggcca gtttataaga tattttatcc gggccatata ttatttcccc gtcataaatc
     1261 aggtggtcca gtttttttaa atgaatatat tcatgaaaag taagtggtgg cagcatgaaa
     1321 tctgtaaatc tgcccgcacc actctcggtg ctgtgccatt ttaaagcggc agccgctgaa
     1381 cctgatacaa taaattttgt ttcgggatag gaatcaacca gaactttaag atgcctttcc
     1441 caatctttaa ggtattgtat ttcatcgaaa aagatgtaac agtcagccat atcttcgata
     1501 ttcaaagcat ttttacagag ctctaaaata tcttcgagtc ccagatgtaa gtaaattggg
     1561 ttatcgatac cgacaaaaat aactttacgg ggatcaacat catctcccag gagctgatga
     1621 atgctgtgat agagcattac tgttttacca acccttcgtg gtcccatcaa taccagggca
     1681 cggcggacac tccgctcttt tacataaggg taaaataatt caaaatataa acggcgggac
     1741 atactgctgt acgtttctga aacctggcgg gtattccacc agggattttc ataacgcaag
     1801 cggtctgtta ttttttcagc aggaataagc tctaaccgtt tcattatttt tcttatttat
     1861 acaaatctat acatttagac ttgttataac aagcatatat ttacattaat gataaaataa
     1921 gtgtttcaac ttgaataatg ccatctaaaa tatctagcaa ctaatccggt aaatactgta
     1981 tcactcaggt tgggatcaaa agctgacatt ccggcataaa atgtacaacc ctaatgtaag
     2041 catccggtta tgtacataat ccatttacgg aatcattgtc attgaaacca ggtactatag
     2101 cgtttggata cctggaaatg agaatactga catcagactt tctataagca aaaatataaa
     2161 ttcagaaatt cggcttccac ggatggctta cataaacata actgcggggt tcccgcaggc
     2221 ccggatccgt ttgattgatt aaacgtacat cagtaaccag gaaacgttag ggaaatatgg
     2281 gaaaccataa ttttctaacc ggacttctgc aagggaacca tatggaatgg aattattaaa
     2341 tttatacaat cttaaaatga ctgtatttat tagatttaaa gattattcaa agaaagagta
     2401 ccgggcctta actgatgatg tattgacgat gcatttacag ggaattgaaa ccgtcggtat
     2461 atatccgctt cttgaagaca atacctcata tttcattgcc gctgatttcg atgacaggaa
     2521 ctgggcagaa tctattctta aactacattc tgtctgctca aagcatgaat taccttctta
     2581 cgttgaacgt tcgcgttcag gaaatggagg gcatctatgg attttctttg aagaaagcct
     2641 tccggctgca aagacccgga aactgatgta cgaattactc agggaggcaa ctataatctc
     2701 tccgtttgct aaagaaccga gttttgacag actttttcct aatcaggatt atcattccgg
     2761 aaaaggaccg ggcaatctga ttgcccttcc gttgaatggg aaagctctga agacattcaa
     2821 ttcagcattt ttggatccgg ctgatcttca gccttacgac aatcaatttg aaattattaa
     2881 aaattttcgc aaactgtctg tttatcagtt taatactatt tatcaggaaa tttttaatga
     2941 aaaggttgat gttttttttc agtcaggttt tgatcggagc gccagtccaa taattgaaat
     3001 ccaaattgat ggtcagatat atttaaagcg ccttcacctt aacagaaaac tcactgagtt
     3061 cattagagaa aaactgaact ttcttaattc tgattatctg gtgaagaaaa acatgggtaa
     3121 atctaccttt actcttgaaa agtatttcag actgattgaa gaaacgcctg atgaaataat
     3181 tttaccaaga ggatttgtag ccaacttaat cttcttttgc aggaaagaaa attacagttg
     3241 caatgatcca gtctctttcg agaagcaaag agattgaaaa aatttccggc agattcggaa
     3301 cgattatcgt ggatgaatgt catcatattc cggcaaaatc attcagagag gcgatagtta
     3361 atttcaattc atattattta tacggactga cagccactcc caaacgtaaa aacaatgatg
     3421 aaaaactgat ttatttttat attggaagta tactatacaa tgcggatcag gttgaacgga
     3481 taaggcaaaa acaaatcaag gtagagataa atatcaagga tacaaattta tacgtacctt
     3541 ttaattacaa aattgataaa tatgaaacca tttcaaagat tctgattcat gatactaaca
     3601 gaaatacatt gattcttgat gatattgaaa agaattcaag ccggttcaaa acatttctga
     3661 tattgacaga gcgtaaagca catgttgata tccttaacct ttatcttaag gaaaaatatg
     3721 aaactattac aattcatgga gaagattctg agagcagcag gaaaagcaaa attgaacagt
     3781 taaagcaggg tcattttaaa atagtaattt ctaccggaca gtattttggt gaaggaatcg
     3841 atttaaataa tctggaatgt cttatgattg catatccctt tgcttttgaa gggaaactgg
     3901 tacagtatat cggacgtata caacattctg gaaatcctcc ggtaattttt gattatcgtg
     3961 attccaagat tgattatttt gaaaaaatgt tcagacaaag gaaccgatat tataaataac
     4021 tcacaaagaa gtaaatatct gaagaccaaa acatagaact taaggaattc aggcgaaatg
     4081 aatattgaag tgaatttacg attttgtaaa cgccagtcag attctctttt tccaaacggc
     4141 tgccataata gatgcgaacg gttgagccat tgtgacagtt aaacacactt ccgtaagcag
     4201 aggaggttaa ttcctctact gagtgattta ctaaaagatg tgaaaaaata tagtagtctc
     4261 gaaatttaag tttgattcca ttattcgact ggctgaaagc caagaacccg gaggggtaag
     4321 ttaagttaat ggtgcaaaga atctgcttgt tacaggatta atcttaacac aattttctgt
     4381 catatttaca ccatcttttt cactttccac ttactcattt gttttattcc tcaaaaggtt
     4441 atcttgaggg atattaacag gcacttttag atgattgaaa cttgaatggc ttataatatt
     4501 gaaatgctgc aactggccga caattatagc ggggtgagtt tcatacttat cagcaaattc
     4561 tattatatca gacaccttca ggtcaattat tttctttaag gtttcaaagg cagcttcggg
     4621 gaaaagcttt ttttgtgcga actcatcagc ttcactttcc ttttcttcat ccattgggct
     4681 tccgtttaca tcttcaagga aaatctcttt tttgccatgc tttaaaatat gtcctgcctc
     4741 atggtaaaat gcaaaccaga agctgtcgtt tgttttatac ctgcctgaca attgtattat
     4801 cgggttattc cctctccacc agaccgcccc tgatacaggc gctttgggta aacaggccgt
     4861 atataaaaca gcaacgccac aacggaaaca tttttcctgt agctgtatta aagaatcatg
     4921 agggtggtca aaaacaatct tctttatttc gttcaatgca cctgaaaatg cctttttatt
     4981 gtattcaggc agtttaagtt gttttgtttt gagttcacct atccgcaacc acgctgaaat
     5041 tgaatggggg ctttgtgtat tcgccaggga aattttaaat gatgccgtga cggattcgtt
     5101 gaggtaaata ttattccact gctcaggtga ggcgacgctg aaaaacttta ataatgcccc
     5161 ggatattgca gccttatcct ttgtatcggg tagccaggtg tcttttttta attctttcac
     5221 tggaaatttt tctgtccatt caatacagcc ggtgagaaat tcttgttctt caatttttgc
     5281 aagatcaagc cggtactctt tttcacgttc caaccagaaa gctgccggaa tatctaagac
     5341 tctctcgagt aaaatcgctg tctttaaggt aagaggttca ctgccgttaa tcagtccgtt
     5401 cagcttctct ttcgaacgcc caatacgttc tgcaagctct gcctgagaca tgccaagggc
     5461 atcaattgtt tcctgtattg tgtcgcccgg tggggataat aattcttttt gtaaatgtat
     5521 atcgggtcgc atagccgggt attttaatgg taatcttctg tgccaagtat ccgtatgtct
     5581 ataattctaa tgcagtcgat gctgttatct tctttaagag gtaaaggatc gtgatcgggt
     5641 tcaaaaatta ttctgtagtt cccggaaata ttaacagcaa attcaccctg tctgtcaccc
     5701 ttcaattcgt ggcaatttgc tgcggggatg gttttcataa cttcaaggtt tgatgaagcc
     5761 cttaattcct tcatacgctg gttaaccttt ttagccatag taccgaatgc tttctgcatt
     5821 tcttttgcca cggtcagttg cctttctaac ttatttgttt tgtaagtaat cttcacagca
     5881 aagataaata aaattaacga aaaaggttaa taaaaccatc aaatcatatg gatattcatg
     5941 gaatttaata ttagttattc attctctaat tctgattttt ctatcggaac aattatatta
     6001 ccatcaatca attcgccttg tattgggata ttaagagttt atttcgaaaa cttaatctca
     6061 aatactcttt acaataagtc ttataattct ctggcccaaa tttgtctaaa cagtaatcag
     6121 caagtacaac attgagtgaa tattcaaggg ttgcagcgca caatataaaa aagccgaaat
     6181 attaatattc agaatttaaa ggctgcgttt aatcttacga gttaaatgaa tgaatatgca
     6241 atatcatgat aaacaaacag atgggtgtat cgctgtttaa gttttataaa ctgggttcaa
     6301 cataggtttt attaccgggt gctgcgcacc gtatgaagcc ctttgtatta ttggctggta
     6361 taattttatt tttctatgag gatttttatt tcagatatga attcttttac ctgatcaact
     6421 aaaggaagta caattttgtc atcgaaatcg tagagatcgc cataatctcc tttttgtcta
     6481 tagtcggcca aatgcgaaag aagttttcca aatttaacat caattattcc tgttttaacg
     6541 aaggtgtcgc taaacttgtt tctgacggtc atagtttaat tccctctttt tttactgctt
     6601 tataaagcgg ggaatacttt aaagtgttgt tccagaatga tatcgggtaa ataaaaacag
     6661 atattatttg accagattca agttcaatgt tataaagaac atctctgaat ataatttttt
     6721 gtaattacct gtctgcctta tcttataagc atacgggcac tatgatacca ggtatcattt
     6781 cctcccgaaa caccacctcc ataatacgca ccgtattgat aattggttgg ttccctcccg
     6841 tcaacatatt taaagttact ttcatggaat aatataagat acttatttga ggcagcataa
     6901 ggataccacg cataagtcaa agcaaaggag gaactgccca catcaatcca gtttcctgta
     6961 ggattaaatt tataccatag gttattccct gttgtgaggt cttgttctgt tccgtagttt
     7021 ggcataatgt ccctgttctt caccctgtaa attactttta tgcgataatc atcccagccg
     7081 gttgaccagg gttgctggtc aagtattaca gcgaactcaa ccggccatgt cacaccgctg
     7141 aggatacgcc agtctgtcca tgctccttcg ctgtcgggat tggcaataaa ttccggtctt
     7201 ccatatgcct cattatttct ggctttcatg aaataggcat aatccctgtt atccagatca
     7261 tcgggtgcat tcacggtagt cggtcgtttg taatacccaa ccattgtcca tccacctccg
     7321 tcggttgtca tatcacaata acaattaaag ggctccagcc ccccctgccc gtcaggatca
     7381 attgtataaa tgccatcagg ggatgaagga ttgttcagca aaatctcctt acagttttta
     7441 tcgtaatgct gtacccgtat accggctata ttgcctattg actcaacggg tattgccccg
     7501 attttattaa tggcattttt ttcggtaccg ctgattttcc ctatttcctg tcccgctatg
     7561 atgggtgcca ggattataag aaacatggat aatatatttt ttttcatccg tggaaatttt
     7621 tgattttcag gttgtatggg cccacccata tttgaaaatc attttcataa atgaatgggt
     7681 ccttgcatat tagttcatgg aagctttatt taatttcaat cagcataagg ttcggattaa
     7741 aataaacagt atttgctttg gaagcataac cgacaatctg cacctgctga cccgtttctg
     7801 aaggtatggt ttgagtcagt gttcccgcag tcggtgaaat gtaaaccggg gcacctggga
     7861 taaagttcca gctatcatta cgtatgtacc cctgaagtaa aacctgcttg cttcctgtac
     7921 ccgtttcaag tgctaatgca acacatggca tggttgaaac agagccggca tccgcttttt
     7981 cgtatttgcc ggctgaattc ataaacagtg cagtaccggc agccatgata tccgtgacta
     8041 tctctccgta gttggtgact ccgcttataa aaaccggttc atttgtctct gaaccctgaa
     8101 caacgaggcc ggatacaaac tcgggtgtat tcccgtttgt cccgtcatgt ccgttacctg
     8161 tgtcatctgt aaccgatgcg gcatttattt ctgctgcaaa gaacaatgca ggttccggct
     8221 tgaaagtccc gtcatagccc cttatctctt ccggggtaag ctttctctgg agccagtgct
     8281 gggaagcata gataatgctt gcttccgcat agaggtcacc ctcgccccgg gaagggtcaa
     8341 catcctgacc accccataac attttatttg tatttccgga atcatcctgg ggaaaaacgc
     8401 cggttaactg acccaccaat tgcccgtcaa ggtataattc aagtactttt gatgttgcat
     8461 catattgaag acataagaca taccaggtca tctcctccag tgatgtattg tatatgatat
     8521 ctctccagcc gtcttttctt atccaggcat gtaatccgat agtgcgtata ttgattgcaa
     8581 tatctccttc cttgtcttgc catgcctttc ccctgaaaaa gtgccagcct ccgccaccgg
     8641 acccggttcc ggcaggcatc atgacacgct caatcactga ccagtcagag ttattcgtga
     8701 aaccctcaaa agtcccaaaa tcaatatagt cttcatcagc actcattagt cttacctgat
     8761 aaatattctc cggttttgtg accatggtcg gtacattgac cagatcatta taatccccgc
     8821 tgaacgcatc accgcttgtt ttggcatata gggcataggg tacgcttaac agctggcttg
     8881 taccggtaat agtgtagcct gttccgccat tggggtcggt ttcggtttta atgaagtaag
     8941 gaccgttggc ccagtcaatt gcggaaaagt tgccacttac aacggtgcca ccgcctatct
     9001 cgatactaac caaaccgttg gcattggtgc ttggtgcctg ggtttccgta taaaccgctg
     9061 taccgctagc cgaaccttgc aaaatactga tttgcatgcc cacttgccgg ttgataacca
     9121 atgcatcgct actgttgcgg attactgcct ggtagctcaa tttttcagga atttgtgccc
     9181 atatggctgc agttaataat attgctatca ggattgcaaa taattttttc atgactattt
     9241 aatttttaat gattttgaat gtttttaact cctttttgcc tgaagtgact ttaataaaat
     9301 aggtggcagg tatcaggtta ccaacggcaa tagttgtttg ctgaccctga attttcctgc
     9361 tttgcaaaag tcttccgttc gcatcataga gatggaagtg gagattggca atatctgaat
     9421 cattcacgtc cagcgtaaga tgatcgcgta caggattcgg atatgccaga aataaaaggt
     9481 ctatgccaat gatttcttca atggctgtta ccatcgaaat ttcatagggt tgttgaactc
     9541 catgaactac cgagccattt gctccggcat aaacagtgta aaaaggcaat ccgacggaat
     9601 agcttgccga accaccactg cccgaggcat ccccgcctgt ggcagggata ccattttgcg
     9661 cctgaattgt gaactcaaaa caaatcaggc acaatataac aagtgtaatg ttgaaaactt
     9721 tcatgattct cttataattg attttcatgc gatattggtc accaggcatt acctgatgta
     9781 ttttttagct ttcacaagtg tcggtattta tcagacatcc ccgctcgatt tcaagccata
     9841 cacaaaatga atatcgtaac acttcctgat gaagattatc agcagttatc cctgattttc
     9901 aggaaataac agtatttcat gaagcatcag aggtaatgaa ttctggcaaa aatatggttg
     9961 ttgccaactc tcatccataa caaatccaat tcattttgaa aaattatctt acaaggtagt
    10021 cagaatcatt tatttatatg tcctggttaa ggaatagtta ttcacattaa attcacggac
    10081 ggaaggggat ttttcctttt tgttatgaga taacttactt ggacatgaga agaaatggga
    10141 tgctgtcagc aactgggttt aaattatctg aaaagtctta aatagttgtc aaacacatac
    10201 actttacctc gcttacttcc agttatttct tttagaatat caagtttttc caaatctaca
    10261 attaatttat atgctgaagg cattgatatt ccagctattt cactaacttt ttctgcattt
    10321 ataattggac gattatacaa ataatcaact atcttttttg cattcactgc tctactacct
    10381 aatttttgaa tacttgattc aattgttttt tgtaattgaa gaatattgtc aaatgttgtt
    10441 attccatttt ttgcagtttt aattattcca atcaaaaaga acttaaacca ttgtgagaga
    10501 ttgtttttat ctcttacaat cattaagttt tcgtaatata actttctgtt tttttcaaaa
    10561 aaatcagata gatacaaaat aggtttcttt aatattcctt tgctgaccag atataacgga
    10621 atcagtaacc tgccaatcct accgtttcca tcaaggaatg ggtgaattgt ttcaaactga
    10681 taatgaacaa gtccgatttt cagcaattca gggatgaaaa tttcttcatt atagagaaat
    10741 ttttcgatat cactcattaa ttcaggtacc gtagtgtgaa ccggtgggat gaaaatagca
    10801 tcattaatag ttgctccgcc tatccaattc tggctatttc tgaattctcc aggttgtttt
    10861 cgttctcctc tgactccttg tagaagtatt ttatgggttt cacgaattaa tcgtgaggaa
    10921 aatggaagtt tatcaagttc gttaatagcc cattccattg ctttcgcata attctggact
    10981 tcctcccagt catctctctt gtctaaaggg atatcttccc tgtccagtaa tgcctcttcc
    11041 atattagttt gagttccttc aatcctgctg ctttgagtag cttctttcag aacgtgcata
    11101 cttataaaga ggtcaacatc aggaatatac tttgagaaca tatctaacct gcctaattct
    11161 ctgtctgctt gacttagtag ttgcaacact tccatattgt caacgtgcaa ttgcttgttg
    11221 attaatgtag gttgaaaact cttatagcaa ccttggctta tgtactgtcc ggatttatat
    11281 tgtttcattt cagttatttt aaacaaaact aatcgttatt taagagaatg caaaataact
    11341 taaataagat atttctttat ttaactttcc cttctaggta gagttttacg tgatgcaatc
    11401 tggaactgct acctttatcc ggacagtaag ttaagctaaa atgcttaatt taacggatat
    11461 gaaaaaaaga agaaagtatg acccggagtt caaacaaatg gttgttgagc tttcacacac
    11521 aagagacgat ctgactgttc tggcaaagga gctggatatt cgagcagaac ttatctatcg
    11581 ttggagacgg gaagcttcgg caaatttggg tgccagcttc ccgggtcagg gtaacaaatt
    11641 gatgacagag gaacaaaagg agatagcccg gttaaaaaaa gaacttcgag atgcccagtt
    11701 agagcgtgat atcttaaaaa aagctgtaag cattttctcc aggagcgatg gcaaatattc
    11761 caattcataa ggacacatac ctgtatattt cccattgaga agatgtgtaa agtattaaag
    11821 gtaagcagga gttcatatta ttgctggctg aagcgcaatc caagcaagcg gtctattgag
    11881 aacatggagt tgtctgaacg gataaaaaag atttacgatc agagtaaagg cacctacggc
    11941 agtcccagga taactataaa gcttcaggaa gaaggatttc atgtatcaag gcagagagta
    12001 gcacggctga tgaaaaagca gaatatcaag agtattatcc gtaagaaatg ggttattacc
    12061 actgattcca gacataacta ccctgttgtt gaaaataagc tcaccaggga cttcactgtg
    12121 gtccgtccgg cacagatggg tgtctgattt gacttatata aaaacgttcc aggggtggct
    12181 ttatttgacc gctattattg acctttatga caggaaagta atcggctggt catacagtag
    12241 atctttgaag gctgctcata caaccattcc ggcatggaaa atggctgtca ggaaccggat
    12301 cataacggac aagcttattt tccattctga cagaggtatt caatatgcct gcaatgagtt
    12361 tacaaatctc ctctcctcgt acaaaaatgt tgaaagaagc atgagtcgca aaggagattg
    12421 ctgggataat gctgccagca gaaagcttct ttaagacact gaaagtcgaa catgtttatc
    12481 atcacagtta taagacattt aaagaagcaa aactttctgt atttgaatat attgaagcat
    12541 ggtataacgt tgaccgtatt catactgcaa taaaaacgtc aatcagaaat aaaaaagaga
    12601 aatttataaa tcaattagta gcttaataaa gtgtccgatt ttttgttgca ggttcaatcc
    12661 tcacgaggta gagttttatg tggtgcaatc ctttcgaggt agagttttac gtgatgcaat
    12721 cgcctcgttg cttgtttata tataccgagg gtgtacaatg attgtttttt agggtagtta
    12781 ttgtgcagct taatttgtat tttttactct ataagcaatt tatgccagat tattccttaa
    12841 attgtatctg caataaaaac cagatttgcc ttaatattcc tgccaatatc tattaaaagg
    12901 aagtccggta aatacaatac aaggctgaaa ttatgccgga agacgtaata aacaccattg
    12961 cagttatagc caccagactt ccctccaaaa aatccattgg ctacaaatga atttgttcga
    13021 agtcggtttg ttcataatcc ttcaatcaga gataatggta tttcagccag ataaattgat
    13081 gaattaccct catgcgagga atagtatgat acgaatattt ttttcctgcc tacaacaaat
    13141 ccgggataac tattatcacc tcctgaaggc agccggagca gatttctgaa ttgcccttcc
    13201 ctgttaacta aaaataatcc ggttattgcc ggtttttcat atatcctggt accggcgata
    13261 aaaagctcgt catttaacgc tgtaaagtca ggtccgccaa accggattcc tgtatctgtc
    13321 caactccact ccttatatgg aggcttgctt cttccccaga agcctgtgcc tgattcccgg
    13381 cgaaccatca tcaacatttc cccgtcaggc attatcctca cagtagtttc gttgggcctc
    13441 ccatccaatg aaagcctggt aaccaggtca taattaatac catcggtagt tttaagaagg
    13501 cagactgacc attcttggtc ttctttttct tctcccgtcg aatatgcaac cccataacca
    13561 atcttattat accatgaaac tctccacagc caatcaaaat ttgttttggc attggctgaa
    13621 ataccaatgg gctggggatc agagaatttc ttccctgaac tatttgaaaa agaaacatga
    13681 gataaacggc tcatgagttt cctgtcttta tatactgaac ctcccatgtt taccagaatt
    13741 cttccgtcag gtgtaacaga caatttaggg tctctgagat cgtaaccttt tttctcaagc
    13801 actgcaaccg atgtccagtt tataccatct tttgatttta taatacgtac gactccatcg
    13861 acacctccgg cgtgatcact tccctgacgg aacgaacaat aaaaactgcc tttgaaccag
    13921 ataagggatg taaaagcatt atggtactca ttactccaga tttttctgac tgaaacatct
    13981 tccggtttaa tctgtgcatt gcttgtggca tgagaaatta tcaggcttat tccaagcagg
    14041 agtgctggtt gtgttaattt tttcattttc ttaaggagtg ttaaactatt aaaattcagt
    14101 atgacaattg atctgaccct gctgatgtaa tcattaccat gaccggaatg ggcgctgtct
    14161 tttctaaacg tcatgtctgt caggaaaggc ccccggttcc ctgtatgatt atcggatctc
    14221 ctttaatgat acagtttgga aaacgatatc ggcctgacta ctttcaaaaa ggataccgat
    14281 ggttgaatca tttaccgatg ttatacagga atatccccgt ccgctatatt catccagcag
    14341 aatatgttta tcttcgggcc atgtattgcc atcatcctta ctcactttca acgtaatatg
    14401 atcccggtta gaggtagaag cgggattggc gaaaagtaat acagatttct caacaccttt
    14461 ttcatgataa acatgtttat gcaggcttgc catacaggtt ggttctatca atgctttcct
    14521 ggaagtggga tgttccgtcc atgtttctcc caggtcggta gttgtggcga tggtccgtcc
    14581 gttcacctca tcatttcccc ggttgcggtt atccctcata ttcaacataa tggatccatc
    14641 ggatagctgg acagccatac attccgtcgt attcttatgt gcaggattac tggttgtcca
    14701 tgttattcct ccgtctttgc tccacgtgat attggaaaac ggtttacccg tctcatcccg
    14761 tccttgtgtc ggaaatacca atgtaccatc cgacagggtg atgccgtggc ccggcgcggg
    14821 agcaaataac caccattcct ttttcttgac agatgtaata tttaccggtt cactccaggt
    14881 taatccgtca tcagtactct tcgtgatcag gaactgtgaa gtttccttta caccgaatcc
    14941 cggttgggac cctttcgcct gccattgatg gatccaccgg atactatctt tggtcatccc
    15001 cttaacccac ttcccggttt ccgggtcgag tacaccatgc atccaaagac ctgctatata
    15061 gatatctccg gtattgtcgt ccaccagaat gcaggcatca cttactccgt tgtacttctc
    15121 cggcaatcca ccccattcct tcatgtccaa cacgatctgc atcggttgcc atgtacgtcc
    15181 tccgtcggta ctacggttca atccaatatc gatatgtccc tgtaaatccc tcgcactttc
    15241 ataccgcgca tcataaattg caagtaatgt tccatccttc gaggtggcca atcccggaat
    15301 acgggaggta tgcacatcgt ccttcatatg ctgacgtact gctacaccta ctctaagact
    15361 ctgtgtatcg tcggaaggaa caggtacttt cccgatatcc gttgtaatac ttttacattg
    15421 taccttgatt ctgtgtgaca gatccatact ctcttttaat gtaaccgata accaaaatgt
    15481 aagtgtatct tcagtaatgt tgaaattatc cttaaacgta acgacggagg caggtgattg
    15541 cactttacct ataagattac cggtggtaaa ccgcccttca ccgtctgatt taaaaagcga
    15601 tacatgctca atatcgttca ggtcagttgt tccctccaac gaaataacga ccttttgcaa
    15661 tgagtaatct gccggctgct ggcggatcat tttgattttc agcacaggat tggcatcctt
    15721 tccggttaac accggaagaa cagggttttc tatctttatg gatattgtat cggtatgacc
    15781 ggtacaggat aataccatta cagaaaggag aaagagtaat attcttttta tcatgtcttc
    15841 tgtttttgta tccacaatct caaagaagcc ctcatccaac ccgacaatct aattcagctt
    15901 atacctgctg tctccggatc ccattgtcaa tctcagcttc tgcatcatca gccaaatggg
    15961 ttcataaaat tcatgaccaa tctgacgtta taactcctat atcattaccg gtagtcattt
    16021 taataaataa actgtatcag ggaaagcccg ttgatacggt tttctaaatg ccggccaatt
    16081 ctgaatttca ggttacgaac ctgaatctgc tgaagcattc cgggatatgg gtgtcgtaaa
    16141 ccccgtgcct gttcatggcc catgcaggat gagggattat ttcctgtccc gagggtgaca
    16201 ttttctggaa acggcccagg aagattctcc agacatcacc ctcctccggc ggaagtgccc
    16261 ttccgtttgc gagccatttc aggcttttcc acggaatggc tatctcggcc gcccatccct
    16321 tgtctgtatc cgaattatca tttatcgtgc cgtctatttt aacagcagtt tcaaggcccg
    16381 gcatatcata gtccaggaat gcccatctca gtcccctcgg atgttttccc caccagaaat
    16441 attttcctgt ccggtcataa tcccctccga aggtcatgga tgttttttta aatacatcaa
    16501 attccggaac atcaaatctt cctccttttt cataggcatc acgccagatg aagaagactt
    16561 cataaatggt gttcagggca tttatttcca gttcataata acagtctccc ccgtcaatga
    16621 aaacctcaag atcattctcc aggaagatta ttgagtcctt ttcagtaagg gtggctgtca
    16681 caaagggctc ttccgcccag aatgcgatat agagatattc ctcattccag agggcagctg
    16741 cacgggtgtc ataaagtccc ggatcgccgg ttaccatatc gacaaagcgc cctgacctgg
    16801 ctgcatttaa ccagaccggc ttagtgaggt cgccgttgat ctctatcctt tcagccgttt
    16861 tgcggcaggt gtattcaaca atgtcctttt cgctgcatcc gtattgcaat tggggtttca
    16921 tgactataaa tcagttgaag ttaaatttgg tttatgatct cagaatgttt tttcctgtgc
    16981 tttgatattc cagccttcag tgatcagtct cagttccccg gccttctgtt ttatacctga
    17041 aggaggattt gcaagtaaag acggaatgtc aagctcaacc gccctgcttt caccgggcag
    17101 caaagtgaag tagttttcgg tgagaaagac cggaagaaca agcttaccat ccgggtcatt
    17161 caccttgagg cgtatgaaga atgcagtttc atcagcagga tttgtaacat ttataatata
    17221 tttattatct gtagatttaa cgatatccgt cttaagtgtt acttcagtca gttcattcag
    17281 ctttgcatat gactttggtt tgtttgaaag ccagtacaga ttctcgtcaa ttattttttc
    17341 cttatcccgg agaatcagtt tcaggaagta aagttcttca gtacttgttt ccggcagaac
    17401 aactttgttg agcaacacaa gatcgtttgc tttcagactt atggttttgc cggattcaga
    17461 tatgatcctg ccgtgaagat catataaagt aacacttgca gtaatattat tcaaatcctt
    17521 ggaagtctga tttaaaacac atacaactga atcgctgaga ttaagctgta tgtgcacggg
    17581 ggcggcagca tgcctgtatc caaaataggc accggtataa tcaaggtagt aatcgtagaa
    17641 gaatcctcgc agtgaggtcc agggattctg atttttccaa acaagcatac cggtgtacca
    17701 gtcccacatt ttatggttga atgcttcctg gagggcacgg tactgctcat agcttaccag
    17761 ctgagccttc atgcagaaat ccctgacatc ctttaccctg ccgtatctgt ccggaaaatc
    17821 catgagcgga agatatttat gataagccca cgatccggat gctctcggag actgcggtgg
    17881 tttcagttcg ttttccggaa tgaattttat caatccgtca tagttgggaa ttcctatgga
    17941 tcctgtttcc ggattaaatg gaaaggatct tacagtgaat atctcttcgg gttccctaat
    18001 gccataggga ccgtcgccca cgccgccgat tatgttggtc atgagtttag gcgaggtaga
    18061 catgtcgagg aagaaccgtg acggatcata tgccggaaag atatcctttt cacatgcctg
    18121 gagaatgtcg gccgggggat atgtttcgtt tcccccgcac cagagataga gacttggatg
    18181 gttccggagc attttgatct gatcggtcag ggattccagg aaaaggtcat gatcatcagg
    18241 atatgctttt cgtctggcct gcgattcctt tttcagtgga tcatcccatt ctccgttaca
    18301 gtcgcctgtt atccagatat cctgccatac caggataccg tatttgtcgc aggcttcata
    18361 gaattccggg cgttctgtca atcctccgcc ccatacccgt atcatgttca tgttcatccc
    18421 agcgtgaagc ctgatttccg cgtcgtaacg ctctttggaa aggcggagca gcgcatctga
    18481 tgctatccag tttcctcctt tgataaagat tttctgtccg ttcacgcaaa agacctgcgc
    18541 agtgattaca ctgtcaaaat agttggatgt ctcccttatt ccgaatgtga gggtttcaga
    18601 atcgaatttc gtaccgttcc tgtcggaata atcgatcttc atgttataaa gaggctgatc
    18661 accaattccg ttcggccacc acagccgggg ttcctcaact tttatctcgg gaaatgagaa
    18721 tactttctcc tcgcggggct caagtaccgc attgatactt accttatacc tgtcgaattc
    18781 caggcgcagg ataccattaa ttactcttga tgtaggattt tttatttcaa cggaagtcct
    18841 gacaaatgcg ggagcctgtt tttctcccgg catccttgtt ccgggaacac gggtttccac
    18901 gtatggattc ctcagatcgg ccgcaccggt gatttcaacg gctacctgat cccaaatacc
    18961 ggtatttcgg tcacggacag gacatatcca gtcccatccg gctgtgaact gcattgccac
    19021 attacggcct attgtcccgt cccctccctg tccggctgca gcattgccgg gaggatcggg
    19081 cggtgctacc aggactgcca gcctgtttgg cccgtccttg ctgatatatt tggtaataag
    19141 atatttttcc ctcaggaaca ttccccggtg ggtgctggca ttcacgcggt ggccgttgag
    19201 gaatatgtcg gcaaagtaat ttatacctct gaaactgagc catgcctgct gaccctcctt
    19261 aagatccgga atctcaaatt cattgtaaaa ccagtacgtg tattcatccc tgccggtatc
    19321 ataaatatcc ggaatcagat tattgttcag tccgtagaaa gggtccggaa caagcttgtt
    19381 atgcagaagc gtggttagaa cagttccggg gacaacagcc tccatccatc cttcaggcat
    19441 taattcactg ccggtaagct cacatccgtc aatcagaacg tcagaagccc tctttgcttt
    19501 ccagttgtcg tgaagaagat aggttacagc ttgagatgaa agctgtaatg acataattat
    19561 caaaataata aaaaataaaa tagttttttt catggatgat attttcaggt ttcgttatct
    19621 gattgtaaaa atatcaatac taatcgattt ttgcttatat tcagtgctga atttcctgca
    19681 gtctgtacag gctgactttg actcggtcac caccagaacc gttttttaac cggacagcat
    19741 ttttaaataa ataacttgtc aattatgaaa aaaaccttgt ttaataatta tctgtcagga
    19801 ttacgctttg ctcttgtttc tgcaattgta ttgccaatgc tggtttcatg tgccacctca
    19861 ccggatgtgc ttgaagatcc tcattccatt ataccgccga aacccgatcc tgcgggatac
    19921 gtatgttaca gaaccaccgg aaacattaca actgacggga tcctgaatga atcagactgg
    19981 gaagcggctc cgtggacaga ttattttgtt gatattgaag gtgacctgaa gccggcgcct
    20041 cattacaaaa cccggctgaa gatgctgtgg gacagtgaat acctgtacat tgccgctgag
    20101 atggaggaac ctcatatctg ggccactctc aggcaaaggg atacagttat attttttgac
    20161 aacgattttg aggtgtttat tgatccggat ggcgatactc acaactatta tgaactggag
    20221 gtgaatgctt tcggcacatc gtgggatctt ctgctgataa aaccctaccg tgacggggga
    20281 ccggcagtaa cgtgctggga tattaaggga ctgaaggtgg gaacccgggt tgatggcagc
    20341 attaataatc cacgggatgc cgacaggggc tggaatattg aaattgctat accattcagt
    20401 gtgctgaggg aatgtaatac cggtcccgcc ctgccggggc agggtgacca gtggaggata
    20461 aatttctcca gggtggagtg gcggacagtc attgaagatg gcaggtacag gaaggaaacc
    20521 gatccgtcaa cgggaagacc ctggccggaa tacaactggg tatggacgcc gcagggccgt
    20581 ataaacatgc atatgcctga aatgtgggga tacctgcagt tctccggtat aaaagccggt
    20641 gaaggcacgg aagtattcgt ccctgacagg gatctgaatg aaaaatgggc tttgcgtatg
    20701 gtttattatc tgcaaaatga atattttaaa aaattcggga catatacttc cgacatcagg
    20761 atgctgggcc ttggcaggga tgattttgag ggagtgaaca cccttccggt catcagggca
    20821 acaggaacaa cctttgaatg ctatttcagc aatgaaaacg gcggacgcgg gtggacaatc
    20881 tatcatgacg ggaagattgt gaatctttcg ggaaaatgac attgatcagt gtttttaacg
    20941 gcgactcatg aagttctgtt tatatgacaa gcccccattt gtccccgccc cggtgtttta
    21001 ccggcggctc acggggtccg gcctgtatga ccagcatccg tttgattcct ccctgtggca
    21061 ttatctgcgg ctcatggagc cggattgaca tgacaggatc ccattcgtca ctgccaggcg
    21121 tggtgaaaag gaattttatt tgagttctga cggacaagta tttttatcac aaagcaagaa
    21181 aaaccttaaa aaattagaaa tgaagacaaa ctggctttta ctatcttttg taattctcct
    21241 gtggttctgt gcaggcaatg acagcaagtg ccagagcata ggcctggttg atgtcagata
    21301 tgcagaacag gtgataacag tcggcggacc gggagctgat attcccggat acacatccca
    21361 ggcagtacag attgcactgg atgcacttaa atcaagagga ggaggcacag tgaagctgaa
    21421 tcccggaatt tatgaaatga ccggccctct tcgcctttca gacaatatga cactgagagg
    21481 atcgggagaa tcaaccgttc tgaggaaatg tgacggattt aaaaccagct ttgtaatcga
    21541 tgccgactgg ggtatgctga aggctgtggt aaaggatgca agcggtttta aaaagggaat
    21601 ggggatacag atctttgatg acagtcataa atcagcctgg gatgtgacca ctgcagttat
    21661 aactgatata caggggaaca ccatatattt tgacaacggg acagtaaatg actatgtggc
    21721 ctcactcaac ggtaccatat cgaacagttt ctcactgatt gaagcagtgg aagcggagaa
    21781 tgtaaaaatt tcagatcttg ttattgaagg aaacggggct gccaatgaat atatcaacgg
    21841 atgcagggga gggggggtat atatgcataa gtcgagaaac tgcctggttg aaaatgtaag
    21901 ggtgaatgaa ttcaacggcg actcattcag ctggcaggta accgaaaata tcagcgtcaa
    21961 aggctgtgaa gcctcccatg ggcgaggact gggctttcat ccgggagccg gttccgacca
    22021 tactgtcata gaggattgta tcagtcatca caacagggga gacggaatct ttctctgctg
    22081 gagggttcag aacggtattt tcagaaacaa cagggtgtat gcaaattccg acaacggatt
    22141 ttcaattgga cataaggaca ctgacaatat cttcgaaaat aaccatgtat atgaaaacgg
    22201 aagacatggc gtctatttcc ggaatgaaaa cgaacagaac ggcggacatc gcaatacatt
    22261 ccgtaacaat ataatagaaa ataacggaat ggcgggtgaa tctgccggtt tccgtatcga
    22321 cggcgaaacc cgcgacataa gtatcatcag caatacaatc cggtcaaccg gaaagggtaa
    22381 gcagactgtt gcaatatctg ccggaaggaa aacatccgga ataatttcgg aaaacaacag
    22441 tatttcaggg tccagggaaa tcgtatatga aaaataggaa cctgcttcag gataccggaa
    22501 agccatggaa gattgtcagt agcggtatcc ctgtatctca aggtttgtca gcccggctgc
    22561 ctttgcatga ttaacggcac tgaagtattc ttcacgagtt attttgcgtg aaatttccgg
    22621 ataatcgaat gccttgtagg taggactgta ctgggacatt atattcaggt aggtgtcccg
    22681 gggcagattt tctgcaatcc agtcaataat tttttccgaa tcacttacct tgtttggcat
    22741 tacaaggtgg cggatcatca gtcctctctg catcagcccc ccggcagcag gtttcgccac
    22801 tcccacctgg cggtgcattt ccagaagggc ctgttttgtc atttcgggat attcccgggc
    22861 accggaggaa tacctggctg ctttctgtcc gtcgtaatac ttaaagtcag gaaggtagat
    22921 atcgacaact ccgtcaagta tcttcagtat ttctgtcctt tcccatccgc aggtattgta
    22981 aacaagaggc accttcaggc cctttgaggc ggccctgtca agagcaagaa gaatatgcgg
    23041 ggaataatgt gtcggggtaa cgacattgat attgggacaa cccattttct gcagtgaaag
    23101 catcataccg gcaaactggt caatgcttct ctttgttcct tcgccttcct gactgatact
    23161 gtaattaatg caaaaaacac atctcaggct gcagtttgac aggaagatgg tacctgaacc
    23221 accttttccg acaagaggcc tttcctcacc gaaatgagga tgagctgatg aaatttccag
    23281 ggtggagtct gcctgacaga atcctttctc tcccttcagt ttattgaccc tgcacatcct
    23341 cgggcacagt tcacagcttt ccatcattgc ccacaacttt tccccgcgtc ttttcagctc
    23401 acccgaccgg tgcagtttaa ggtatgaagg ctcgaagttt ttttcctgcc ctcctgcaga
    23461 aaaggatatt cctgcagaaa aggatattcc tgaaaactta ccgggaatca ttcctgagaa
    23521 agtggagtaa ataagcgatc tgatcatatt ccttctggat acaaatttca ttttatgcag
    23581 ttgacaaatg ctgtagatgt tatttgatat ttaataataa gctcttttct tcatatttac
    23641 aaaactcaga acaaaacagg ctgaaagcat gtcttcattt gggaaacgct tttcttttac
    23701 aacctcacaa aacttaaaat acaacaggct gagggcatgc cttcattcgg gaatcacttt
    23761 tcttttacaa cctcacaaaa cttaaaatac aaaaggctga gggcatgcct tcattcctgg
    23821 aacgctttcc tttttattta ctcacaaacc tgaaacagag agtgccgctg acagcaccta
    23881 tagttttctc agcggcttct ttcccagttt tctcaggaca ttatctgacc accggtaagc
    23941 ctcaggaata tctccgacaa aaatgatctc agccggtgaa aggaatgccg gcagctggta
    24001 gacatccgtg attcttaaac aattcagtat ctcaattgct gctcccctgc catccggact
    24061 gcttgccaca tcctggctgg ccggaggatc cttcagtatg atgcttttgc aatttccgtc
    24121 tagcagggcg gcgtatagtg caacaacagc catggcatcc cttgcagcaa tcccgattga
    24181 atcggcattg acgccgccga gagtcctgca gaacccgatg cagcgcataa gatcatacac
    24241 ctgcattgat gcaggagtcc ttcccagcca tgccgatgca cggcgaatat gccactggag
    24301 attcggatcc cagccgtatt cgcctgcgcc tctcacttca aaatatacaa tattccagtc
    24361 tttcccggga tctccgatga atccttccga ctcccaccgg ctttcgcccg gattgcgtaa
    24421 aacgatcatc agaggctttt tctgtgaggc ttcgttcctc cagcgtatat caaccttcag
    24481 ccgccagcct tcctctgaaa cgaaactgta gatatcactg ccgtaagatg ccctgtcgag
    24541 cgttctgaac acttttacag agtcaaaggg aggagggttt tgaggaaatg cggagaaact
    24601 cttttttctc agaaaatccg tgacagcctt ccggtgagca atcagatcat cttccgaagt
    24661 aatctccgga tcaccggcaa gttttacaaa ggaatcctgt attgtggttg tccggtcatc
    24721 cgcaggcgct cctgccgtat atacccttaa ttcatcttct gaaagcatat ttccggggga
    24781 agtatcaata tccccggctt cctcggggga taaatttttc cccataagat gccgtgcaaa
    24841 gaatgaaaag attttttccc tcgaattttt atgataggca tgaccaccgg gtgtttcaat
    24901 atattcgata tcagctgctg cgttgtggag ctcatagata cggcgcaggt ccctgtatat
    24961 ccgccgtaca gactctatct gatttaaacc gtcacggtcc gactgtccta tcagaagggg
    25021 ccttggtgct atcagggcgc cgatatcctg aaaatctata agtccgctgt ttatagccat
    25081 catgcagtcg cagtgatcat cgatggttcg tgttgttatc tgtgcctcca ttgtgcttgc
    25141 cccgcataca ggggcggagg ccttcacccg ggtgtcagcg gcagccagga accagctgat
    25201 ggctcctcct ccggatattc ccgtggctcc aaggttttgt tcatcaactt caggtctttc
    25261 tgaaagaagg tccagacccc gtatggcatt ccatacttcc acaccggcag gagtgtatcc
    25321 ccggctgtac cagttgaacc atccccttgc atagcagccg tggtgttcgc cgcggacttc
    25381 cccgtactgt atggtttcaa caatgaggca cacaaagcca agccgggcaa atttggccgg
    25441 atgagcctgg taatgcactt tttgcgtgcc tgaatgaccg cagaggtaaa tgattccggg
    25501 cacaggctca cttacattgt cgggaatata gaggtttgcc ggaacataga ggccgggaag
    25561 cgattcatag taaagctttt ctattctgta tccatctctc cggactgttc cggtgatcct
    25621 gacattcagc ggaggtcttt ccccggtcag aggcatatcc tgcagactta tcatttcgat
    25681 aaattcagaa tgccgttccg gtctgacctt ttcccattcc gatgccgata aaacagtgct
    25741 tccggaattg tctgtaatat cctttgcggc ttttacaaga taattccgta tgttattagc
    25801 catttcacct gaattgtcgg gagcagcctg cttttgcgaa caggagcagg tgaggacggc
    25861 cagagttgcc agtagcaggt attcaggaat ttttttcatt tt
//
LOCUS       DAOSPX010000009        25272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2150_length_25272_cov_3.896697, whole genome shotgun sequence.
ACCESSION   DAOSPX010000009 DAOSPX010000000
VERSION     DAOSPX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25272
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2150_length_25272_cov_3.896697"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1637
                     /locus_tag="PLZ75_01015"
     CDS             198..1637
                     /locus_tag="PLZ75_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOS71009.1"
                     /translation="MINPLIIARILSTILLIESLCFLVCIPVSLSYSEPSRPFLLSSA
                     ITGSLCFILRMLSLRTGITKISIRDSYITVALAWIILSLAGTLPYILSGTISSLNDAF
                     FESASGFTTTGASVITDVEILPFSILFWRSFTHWIGGIGIIVLVIIILPALKLTAHQL
                     FSLESSLKEKIQPKTKTVGAILLMAYTGLTIIQILLLWAGDMNLFESVCLTFGTVATG
                     GFSVRNTGLISYSPYSQYVIAIFMFLSGVSFVVYYYLIKRQFHKIRQNNELVFYTAVV
                     LISGTVISLMLLSAGDKETEPAFREGFFQVISIITTTGYTTVDYLTWPSAGLLIIFVL
                     MFAGACTGSTTGSIKMARHLIVLKNIRLLFVRLIHPNAVIQLKMNGRPVSDRVNMSIL
                     TFVLLYVVIFLSATLIVVLTGVDTVTSAGAVASTLGNVGPGLGAVGPMFNYNHLPEAD
                     KLLFSLLMIIGRLEINTLFIIFTGSFRKM"
     gene            1954..3990
                     /locus_tag="PLZ75_01020"
     CDS             1954..3990
                     /locus_tag="PLZ75_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71010.1"
                     /translation="MDLTKNYLKIKPSEQSVDYVENKKQFHLYSLLTEQRHPKTWNLS
                     SSIKDDTAKGLQMLLSVDEDITRKTLELADNIQPLEQAADAIYRAMQEGRKIYYYGCG
                     STGRLAKQMESSFWRPFWRKMKKSHLWEKVKDHVPENIEDYLIGEMTGADRALISSLE
                     GFEDLQLIGSLQLLDRGIEKGDVVFCVTEGGETSSVIGTILKALDQYGELTAETSEEA
                     RRNLYFVYNNPDEVLMPFDRSRTVIENPAITKLNLTTGPQSITGSTRMQATTSEIFVL
                     GSVLEEALYKFLSNHLSKSELSTIGFIEGITLKKRIASFREVKESVDKTVASLCRLTE
                     AEAETYKRGRFATYLASEALVTVFIDITERSPTFRLFPLDTVKSRDRRSWIQVWAKGK
                     NTEEAWFNFLGRDFRGLEKSFYETPFREKIEDPYLKEVALKSLENAGNDQKELYDLSF
                     TTWNIENRGPSAGDLGIIVCIDDEVAEINNPESDFRKFADLFREKGGRLAVISVSEEH
                     KTKGSNNFIPDIEVEITLPSFPDPVTLRKQIALKMLLNAHSTGIMAILGRIIGNTMTT
                     LNPSNLKLIGRATYLIMSHVNDTIAQKEWIGKYGYHEPLSFDEINAVLFDSIDYISSR
                     DAGETAEVPLSIIRILESLKTKGFVSWDETRQILDRQGLENYLMEHNPNLTSGK"
     gene            4012..>5145
                     /locus_tag="PLZ75_01025"
     CDS             4012..>5145
                     /locus_tag="PLZ75_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71011.1"
                     /translation="MNFTIIDALIVIVYMAGITLFGIKLAGKQTSTRDYFLGGRKISW
                     WVVCFSIVATETSTLTFISIPGLAYLTNFNFLQLTFGYFLGRIIVAWIILPAYFKGEL
                     GTAYTFLANRFGTPMKNLASGVFMLTRLAADGVRLFATAIPLALILQGTFIGKSLSGD
                     WIYIWSIILIALVTYSYTFLGGLKSVVWMEMVQLFIYITGAIIAIVIVSSKLTGGMPA
                     GIETAAEAGKYQLFNFSFSGGLKGFFSTPYTFIASILGGMFLSMASHGIDQLIIQRLL
                     ATGTLKASRKALVTTGGIIIAQFALFLFLGTLLYVFYGGADIRSDEIFPKFIIEEIPT
                     GLSGFIIAALFAAAMSTLSSSITALGSTTMYDYVMPYYKKLNPA"
     assembly_gap    5146..5155
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5156..5491
                     /locus_tag="PLZ75_01030"
     CDS             <5156..5491
                     /locus_tag="PLZ75_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71012.1"
                     /translation="ISRMITGIWCLLLVGSAVSFMKSSQVVVELALSIASFTYGGLLG
                     TFILGVLFRKPLIRQAVPAFLTGIAVMVYIIIFTSIAWTWYTLIGTLATVLTGLVLTW
                     IAQNRKANE"
     gene            complement(5672..6385)
                     /locus_tag="PLZ75_01035"
     CDS             complement(5672..6385)
                     /locus_tag="PLZ75_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS71013.1"
                     /translation="MKAMILSAGKGTRLGKITESIPKVLVDINGKSLLRRAVEKCVSH
                     GFDDIIINVHHFAGLVEEEIVRLRREGFRISISDEREELLDTGGGLYRARHFFDRTPF
                     LLYNADIITDFDLGILLDYHLEKGGLATLVVRHRPGTRFLLIDSEGLLRGWCNKQTGE
                     RIISRGSGENLSEIAFSSMALIDPEIFSYMSEGVYSMTPLYLQLAAERNIYTLLSDSG
                     YWFNAGTPEILEEVRKFLV"
     gene            complement(6396..7430)
                     /locus_tag="PLZ75_01040"
     CDS             complement(6396..7430)
                     /locus_tag="PLZ75_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOS71014.1"
                     /translation="MDVISKVSQGYREYSGNAVTKTEALPRSGSDRRYFRIFDGEKTL
                     IGTYNRDREENDAFVGFTEHFLRKQLPVPEVYCYMPEKFIYFQRDLGDLNLYTWLHAR
                     PDISDFNDETKQLYKKILDRLILFQTRGIDGLKLDLCYPHKSFDRQSMMWDMNYYKYM
                     LLKLLAVPFRERPLEQDFNNLADYLLETGQKYFLYRDFQTANIMLIDNEPWFIDYQGG
                     RKGAPQYDLASLLFDAKIPMKQENRDELADYFQEKFCTVTNEDKQKFRGYYSGFSMIR
                     LMQALGAFGFRGLYEKKPTFTETLVPAVILLQDIAAKAEQHIRLPELYRAIAATSDTT
                     LFKTLETKRQ"
     gene            complement(7651..8760)
                     /locus_tag="PLZ75_01045"
     CDS             complement(7651..8760)
                     /locus_tag="PLZ75_01045"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOS71015.1"
                     /translation="MAKSSGRLVSLDIFRGMTIAFMIIVNTPGSWDYVYPPLRHAAWH
                     GCTPTDLVFPFFLFIVGVSLWFSMKKYGQELNSGSLLRIFRRTVTIFALGIFLTIFPH
                     FVRDYSSLRIMGVLQRIALAYCLGSIICLSVNRNYLWVVIAVILLSYWAVLAFAGGTD
                     PYSLKDNFALRVDIAILGEKHLYRGYGIPFDPEGLFSTLPAVATVLIGYYAGELLGKG
                     SAGWDKVVKLLLLGASSTGLGLLWSIFFPLNKPLWTSSYVLYTAGIAMGVLALIYLIA
                     DVWKLRKWGTFFLVFGTNALFSYFLAGIWTRIMLNVKVTAGGSEMNLYNWIWEKICVP
                     VAGNMNGSLMFAILQVLLIWIIALVLYRKKIMIRL"
     gene            complement(8784..9875)
                     /locus_tag="PLZ75_01050"
     CDS             complement(8784..9875)
                     /locus_tag="PLZ75_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOS71016.1"
                     /translation="MISSTFTSCGNNSGGKTGVRSRKTSPIKLTYTPYTLQLKHVFTL
                     AAGSRTTTPVVLTKLECDGFVGYGEASMPPYLGESHESVQKFLSRVNMEQFSDPFLIE
                     DILEYVDGIAPGNYAAKASIDIALHDLAGKIMGQPWYRIWGLNPEKTPCTSFTIGDDT
                     ADVVREKVAEAEPYKILKVKLGVGNDKNIIETIRSVSDKPIGVDVNQGWKDRNYALEM
                     AHWLKEQNVIYLEQPMPKTMTEETGWLTQNSPLPVIADEAFQTKDDLFRVKDFYDGIN
                     IKLMKCGGMNAGYKLALMARAMGMKIMVGCMTETSCAISAAAQFSPLVDWADLDGNLL
                     ISNDIYDGVTVEDGRIILKDRPGIGIIEL"
     gene            complement(9920..11107)
                     /locus_tag="PLZ75_01055"
     CDS             complement(9920..11107)
                     /locus_tag="PLZ75_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing C40 family peptidase"
                     /protein_id="HOS71017.1"
                     /translation="MKHLFRILLILLFISGCKPSEVPLSLQNEIDSIVLQYIPDKREA
                     LCNLDMEILKDRRLLIKGETSIPELKAEILKHLEQSGTGYADSIKVLPDKSETARSWG
                     LICVSVANLKKDPSHSSELVSQTVMGTPVRILKKEGSWLLMQTPDHYLGWTTASSVSG
                     MDESGFQEWKESDRLIYTSRTGDILSEGSGKEVVSDVVTGSILKRISAGRDYFTVELP
                     DGRKGRINRKDAEDFEKWRQTVRPEADKLVSFAESLTGVSYLWGGTSTKMLDCSGLVK
                     TAYFTGGIILARDASQQFRYGEEVDISSSFEALRPGDLLFFGSVNDKGEKRITHTGMY
                     IGNNEFIHASTGRGMVANNILDPDAGDYDRYLVSILQGARRIIGVPSGKGLEHISQNN
                     WYR"
     gene            11329..12573
                     /locus_tag="PLZ75_01060"
     CDS             11329..12573
                     /locus_tag="PLZ75_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOS71018.1"
                     /translation="MFALRIVLIALILGFQWEDGMNSRAVETGLEVLTGDDFSILKGK
                     RVGLITNPTGVDRNLRSAADILHNAPGVRLTALFGPEHGVRGEFAAGETIGPAVDPAT
                     GIPVYSLYGKTRKPTPEMLRNIDVLVYDIQDIGSRSYTYISTLGLAMEAAAENNIPFL
                     VLDRPNPLGGIRMEGPLVQPGFTSFVSQYPIPYIHGLTVGELAEFLNGEGLLENGVKC
                     RLEVVKMKNWKRDMLFEDTGLPWVPSSPHIPNAQTALFYPATGILGELYTVSIGVGYT
                     MPFQLLAAEWIDADSLAAALNALSLPGVRFRPLHYKPYYSVAQGTMVHGVQIHLTDPA
                     KAPLTLLQFYFLQEVRKLRPDKDIFSLCDPSRLDMFDKVCGTDRVRKEFLKTWQVSSV
                     LDIWNGDIPQFRKKAEKYLLYK"
     gene            complement(12602..14923)
                     /locus_tag="PLZ75_01065"
     CDS             complement(12602..14923)
                     /locus_tag="PLZ75_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71019.1"
                     /translation="MTNVELTFQKRSGRTGFSTKIPYLVVDNFPALGLLTSLRFLEWV
                     SENPEGLISLPTGKTPEYFLKWTRRFLEGWNRPENRRIMEENGFTIRKKPVMKKLRFV
                     QMDEFYPIDPGQHNSFYNYVLNFYIKGFGLDPAAALLMNSDEIRLAEGMHYSRVFPGN
                     RIDLSLRNREALTPHEKIQQASLFRIDKWCMEYENRIRETGGIGFFLGGIGPDGSIAF
                     NSRGSDHNSTTRLTATNFESQAAAAGDLGGIEISRNRLVLTIGLGTLTYNPECTSIIF
                     AAGESKAHIIKNALENEPDNLYPATVLQRLENARFYLTRGAAVQLHDSIESYYRQGDW
                     TFEKTERAVYDLCRKIDKYAHNLELDDLRNDKYCSLIPGLSLKAVQDVIISTKRKIER
                     GSIRENDQVFLHTGPHHDDIMLGIFPSIISQLRNRSNKFHFAVLTSGFTAVTNEMLEN
                     LLVETLEHIEQGKIQMLAYPDFFENGYRYKFDKDVNHYLNKVADRDEKGKLRGVCHRI
                     TRVMVGIYNLKSREDLVNQLQANVETLRNSYSGEKNPPDIQKLKGMLREFEEELVWAH
                     YGVRVKNIEHLRLGFYTGDIFTSQPQKTRDVMPILKMFRKIQPTVISLAFDPEGSGPD
                     THYKVLQAIAHALIQWKKEKDLKDLRIIGYRNVWFRFHPSEANVFTPVSLNSMAVLDW
                     SFRNCYISQVDASFPSYELDGPFSDLSMQIWVEQFKRVQLILGKDFFYDNESPKIRAT
                     HGMIFYREMSIDEFLVHARELEKSTEGNSPEWH"
     gene            complement(15167..18094)
                     /locus_tag="PLZ75_01070"
     CDS             complement(15167..18094)
                     /locus_tag="PLZ75_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOS71020.1"
                     /translation="MNKMKLDSLRAGLKGDLFTDRKHRILYATDGSAYRELPRAVVRP
                     KDKDDIRKVIAFARAIKGSVIPRGAGTSLAGQVVGPGIVIDVSKYMNRIIEINPEERW
                     AVVEPGVVLTALNRKLAPHGLMFGPETSTADWCTLGGMLGNNSCGAHSLIYGSVRDHI
                     LEADVILSDGTETTFRALSRKEFDEKCSGEKDSLERSIYRFIKDILSDSVNRDEIRAQ
                     YPHPGVKRRNNGYALDSLLETDPFSGNGEKINLCRLLAGSEGTLAFTTRMKLNLIPVE
                     SRKTGLITAHFMSLDEAVRANNLILKHEPAAVEMIDDFIVECTKDNIEQNKNRFFIKG
                     SPKIILLIELLKNTEKELKKCASDIEKELTAAGYGYHFPLFTGEDEIKRIWDLRKAGL
                     GVLLIIPGEKRSVQVIEDTAVLPEYFPEYIEEFQQILKKYSLNCAYYAHIATGELHLS
                     PLLDLKDPADVDIYRNLARDVAQLVKKYRGSLSGEHGDGRLRGEFIPLMLGDHIYNIL
                     RKLKRTWDPENIFNPGKIVDVPPMHTSLRYRTGEHIEPLDSTFVFPDKGTILSAAEKC
                     SGSGDCRKSPLIGGTMCPTFMATEDEDKTTRGRANILREFLTRSTKKHTYNHREIWQV
                     MDLCLSCKACKSECPSNVDMTKLKAEFLSHYYRLHGIPIRTLLIGWLPRINRFGHAIW
                     PLTNFIMRQSFFKKLIGFNPDRTLPSLSKITLRTWAEKCQTTSMHMKNPLGKVFLFAD
                     EFTNYNESDIGIKAIILLETLGYQVEVPAHAESGRTFLSKGLLKSARKIAEKNVRMLK
                     DIVTEKTPLVGIEPSAILTFRDEYPDLLSGELHDAAVKLGKNAIMIEEFICREYDKGK
                     ITPEQFVHDPLSVKLHGHCQQKAIASTSFTRKMLSIPENYEIEEIPSGCCGMAGSFGY
                     EKEHHELSMKIGELVLFPAVRAASEETVIAAPGTSCRCQIYDGTGKKAVHPVEIMYDA
                     LMR"
     gene            complement(18087..19751)
                     /locus_tag="PLZ75_01075"
     CDS             complement(18087..19751)
                     /locus_tag="PLZ75_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS71021.1"
                     /translation="MERKAYRSPWAWVPTLYFFQGVPYSIVMITSGLIYKTMGISIAS
                     LAFWTSLLYLPWAVKPLWSPYIDVVSTKRNWVVWTQLLLGIAFIGAGIAMPVSFFYPV
                     TLGIFAIIAFASASHDIAADGYYMYALDQHEQSFFVGIRSTFYRFAMLTALGLVPVIA
                     GVVQKNTGLESVTFEAVSVPADEYVPFDPSAIVIEESAGEPAILLFPRNLQIPMFRTG
                     ESEVDSAVVYIALSAPPGHGKTVVVNASRNTGSKDIDIAANQTGRYEFSDSNWDKPVS
                     LTLKINRNLDERTSAGFLVTAGNIAFSWTVALGIIGLILLLLAVYNRILLPYPPENDN
                     REKIGWKVYKEVFVSFFTKPGIIPALIFLLFYRLGESQLVKVATPFLVDSRSSGGIGL
                     TSTEYGIAYGTIGMLCLTIGGILGGIAAAKYGLKRLIWIMAISINLPDAVYIYLAYVQ
                     PMPGNPGIYLAIALEQFGYGFGFVAYMLFMLYFVGESRYKTAEYAIATSLMALGMMLP
                     GMVSGKVQELLGYQHFFIYVLICTIPGMVVIKFLKIDPSFGIKKNE"
     gene            complement(19761..22229)
                     /locus_tag="PLZ75_01080"
     CDS             complement(19761..22229)
                     /locus_tag="PLZ75_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOS71022.1"
                     /translation="MNKRSIIILIGLFLAGTLSGQSWIRINQLGYLPQSVKVAVCIST
                     DGLSDKYFTVHNAITGTVVFKDKARHYSGEEWGMRSAARLDFSKLETPGGYFIRFGNA
                     ESEAFSISADVYRGTSDFLLEYMRQQRCGYNPFYGDSCHVHDAIIVDHPEKSGQRIDV
                     TGGWHDATDYLQYTCTSANAVYQMLYAYRRNPEVFGDSHDASGNPGSNGIPDILDEAR
                     WGIDWLLKMNPDSGEMYNQIADDRDHAGFRLPAGDKASYGLGVHRPVYFVTGKSQGLS
                     RYSNRSTGVSSTAGKFASAFAIGAEVFRDIDSGLADRLRKKAEEAWRFGLTDLGTTQT
                     ACVVSPYFYEEDNYVDDLELAAIELFSLTGDSAYLVQADYWGTLERISPWIEMDTARH
                     YQFYPFVNLGHAGLAKSGTRYSGKFISFMRRGLQILEDRKKNDPFRIKIPFIWCSNNY
                     VTAALTQYRIYAEVSGDSRFAETEAAHRDWLFGCNPWGTSMITGLPEGGDYPRYPHAS
                     VTVLSGIIPPGGLVDGPIYRRIYDQQIGLTLTRPDPYAPFQNGKAIYHDDTGDYSSNE
                     PTMDGTADLTIYMSALESEGLRQTGRPGEEVIDDQGAIIRKNNKKEIYLLFSADDHND
                     GFDHILDVLSKTGSGASFFLTGNFLRNPGNEKYIRRIVSGGHFAGPHSDRHLLYAPWE
                     KRDSLLVDREQFVSDLRANYRELAKRKAGKPGTLYFLPPYEWYNSAIAHWTSSLGIKL
                     INFTPGTGTNADYTTPDMSNYRSSDELLEGLKRYEASDPRGLRGAMILIHPGTHPDRT
                     DKLYLKLGEIITFFSAKGYIFKSL"
     gene            complement(22342..23685)
                     /locus_tag="PLZ75_01085"
     CDS             complement(22342..23685)
                     /locus_tag="PLZ75_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOS71023.1"
                     /translation="MSSQDKIQELHVGILSAREISFTLNGKYESDPGNLVLGDKCRAF
                     YRNGKIVLVSEEREISSTGQIILKPAGSDMSFTLHDVVIGIGFHWERTEDQVFKGSLK
                     FIAEEDRITAVNLIHIEEYLKSVISSEMKATSSAELLKAHAVISRSWLLAQIEKSRKN
                     RGENSPVRTEFISDTEITRWYDREDHTNYDVCADDHCQRYQGITRAASPVVAEAVEET
                     IGEVLVYGDEICDTRFSKCCGGITELFENVWGPVHHPYLQSITDNASSPPGSSQAPVD
                     NERAEDWIRSSPEAFCNTTDKKILGQVLNDYDMETMDFFRWKVTYGQDELSDIIRTRT
                     GIDFGTVTGLVPLERGSSGRITRLKIEGTKKNLIIGKELEIRKSLSKSHLYSSCFYVE
                     KTESGKETKFILHGAGWGHGVGLCQIGAAVMASEGYRYDEILMHYFRGAELKRIY"
     gene            complement(23791..24861)
                     /locus_tag="PLZ75_01090"
     CDS             complement(23791..24861)
                     /locus_tag="PLZ75_01090"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOS71024.1"
                     /translation="MHSNTYSKRVSEFFLAQLGEWELAARNFRQLEKVKIRHLDFGGF
                     EVLVQFNPERYRSTAAKTDSGSIEARPCFLCERNRPPQQRGLPFDDQFTVLLNPYPII
                     RRHLTIVSNSHVEQRIMNNFGKMLSIAAALPDYVVFYNGPQCGASAPDHLHFQAVSRG
                     FMPLEKDFESQRFTHVVSTGPGYEILYWTGYLRGIVTLKGREKEILENLFESVYEKLS
                     LLTVNRAGSGQGSELSLRDQPEHTTEFRSPGDDSRLPGTGNPADQSRNAEMEPMMNIL
                     VYRNTENLIVHIIPRKKHRPGQFFAAGSDKITISPASVDLGGLVITPREEDFDKLTEN
                     DISDIFRQVCVGEDELYRMLKG"
     gene            complement(24986..>25272)
                     /locus_tag="PLZ75_01095"
     CDS             complement(24986..>25272)
                     /locus_tag="PLZ75_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS71025.1"
                     /translation="RINGLGAPRAFYTPVIRSIGIPDVSYGEDYAAGLAISRNYQIGR
                     IYESLYLCRRWEGNTDAALDIVRTNANNLYKDRIRTMELMARKRKVSLQR"
BASE COUNT         6541 a         6192 c         5494 g         7035 t
ORIGIN      
        1 gaacaatagg accgatgttc acctattccc acatgacgga tttcaacaag atattcttct
       61 gtatcctcat gataaccgga agactggagg taaatactct atttattctt ctttcaagat
      121 cattctggaa agactgaatc cggaaataaa ccggattgag tatctttacg ggccgtttga
      181 ggggcattaa aatgaaaatg atcaatcctt tgattattgc aaggatactg agtacaatcc
      241 tgctgatcga atccctttgc tttttggttt gtattcccgt ttcactcagc tacagcgagc
      301 cttcccgtcc gtttctgctt tcttcggcaa tcacaggatc cctctgtttc atattgagga
      361 tgctcagcct caggaccgga attacaaaaa tcagtatccg ggacagttat atcaccgtgg
      421 cactggcatg gataatcctc tccctggcag gtacacttcc atacatcctg tcaggaacca
      481 tttcatcact taacgacgct ttcttcgaat ctgcttcagg attcaccact acaggggcat
      541 cagtcatcac cgatgtggaa atacttccct tttccattct tttctggaga agttttacac
      601 actggatcgg gggcatagga ataattgtgc ttgtcattat aatacttcct gcccttaaac
      661 tgacagctca tcagctgttc agcctggaat catcccttaa ggagaagata cagcccaaaa
      721 caaaaaccgt gggcgccatc ctgcttatgg catatacagg tctgaccatc atacagattc
      781 tcctgctgtg ggccggagat atgaatcttt ttgaaagtgt atgccttact ttcggaactg
      841 ttgctaccgg aggattctct gtcagaaaca ccggactaat cagctattct ccatacagcc
      901 agtatgtcat tgccatcttc atgtttcttt caggtgtaag ttttgttgta tattattatc
      961 tgataaagag gcagtttcac aaaatcaggc aaaacaatga actggttttc tatacggctg
     1021 ttgttctgat cagcggaacg gtaatttccc tgatgctgct ttctgccgga gacaaagaaa
     1081 ctgagcccgc attcagggaa ggctttttcc aggtcatttc aatcattacc acaacgggat
     1141 atacaaccgt ggattacctg acatggccat ctgccggcct gttaattatt ttcgtgctta
     1201 tgtttgccgg agcctgtacg ggatctacaa cgggaagcat taaaatggcg aggcatttaa
     1261 tagttctcaa aaacatcaga ttactttttg tcagacttat tcatccaaat gcagtaatcc
     1321 agctgaaaat gaatggcaga ccggtaagtg acagggttaa tatgtcgata ctcacctttg
     1381 tgctgcttta tgtcgttatt tttttaagtg ccacgcttat tgttgtgctg acgggtgtgg
     1441 atacggttac atcggccggc gcagttgcct caaccctggg aaatgtcgga ccgggactgg
     1501 gagcagtggg tcccatgttc aattataatc atcttcctga agcagacaaa ttgcttttca
     1561 gtctgctgat gatcatcgga agacttgaaa taaacaccct cttcataatt tttaccggat
     1621 cgttcaggaa aatgtgagat cctgtttcag ggtttccaat ccattaaatg atatttacag
     1681 ttcatgtttc agctgcctgc acaatatcat gcaagcccgg ttactgacaa tattgtgccc
     1741 gggccggcgt atctccggag ccgaaactga aaaccgcttc tcataaacag aatccttcac
     1801 ggaatcatct gtttgaaaga aaattaaaaa aatgattatt ttcggggaga tttttatgaa
     1861 ataaaggaga ggaagaaagt gtcctgccgg aacaggacac agtgccgtaa atcattggaa
     1921 aaacccgttt cagcagcata aaacaaaatt attatggatt taacaaaaaa ttatctgaaa
     1981 attaaacctt cagagcagtc ggttgattat gtggaaaata aaaaacaatt ccacctctat
     2041 tcccttctta ctgaacagag acatcccaaa acatggaacc tcagttccag tataaaagat
     2101 gatacggcaa agggtctgca aatgctcctt tccgtggatg aagacattac acgcaaaacc
     2161 ctggaacttg cagataacat acagccactt gaacaggccg ccgatgccat atacagggca
     2221 atgcaggaag gccggaaaat atattactat ggctgcggat caacgggccg gcttgcaaag
     2281 cagatggaaa gctccttctg gagaccgttc tggcggaaaa tgaaaaaaag ccatttatgg
     2341 gaaaaagtga aggaccatgt tcctgaaaat attgaagatt acctgatcgg ggaaatgacc
     2401 ggggccgaca gggccctgat aagttcactg gagggtttcg aagaccttca gctcattggc
     2461 agtcttcagc tccttgacag gggcattgaa aagggagatg tggtattctg tgtaacagag
     2521 ggaggagaaa cctcatcggt gatcggtaca attctgaaag cactggatca gtacggggag
     2581 ctgaccgcgg aaacatctga ggaggcccgc cgtaatcttt actttgtata caacaatccc
     2641 gacgaagtgc tgatgccctt tgaccggagc aggacagtga tagagaatcc tgctatcacc
     2701 aaactgaacc tcacaaccgg tccgcagtca atcacaggct ctacgagaat gcaggccacc
     2761 accagcgaga tcttcgtcct tggttcagta cttgaagaag cattgtacaa gtttctaagt
     2821 aatcacctca gcaaaagcga attgtccact ataggcttca ttgaaggaat aacactgaaa
     2881 aaaagaatag cctcattcag ggaggttaag gaaagcgtgg acaaaaccgt tgcttcactt
     2941 tgcagattaa cggaagctga agcagaaact tataagcggg gccgtttcgc aacatatctg
     3001 gccagtgaag cccttgttac tgtttttatt gacattaccg aacgaagccc tacgttcagg
     3061 cttttccccc ttgatacagt aaaaagcagg gacagaagat cctggataca ggtatgggcc
     3121 aaaggaaaaa acacggaaga agcatggttc aactttcttg gcagggattt caggggtctt
     3181 gaaaaatctt tctatgagac tcccttcagg gaaaagatcg aggatccgta tcttaaggaa
     3241 gttgcattga aaagtctgga aaatgccggt aacgaccaga aggaactgta tgacctttca
     3301 ttcacaacct ggaatattga aaacagaggt ccgtcagccg gcgatctggg aattattgtc
     3361 tgtatagatg atgaagtggc tgaaataaat aatcctgaat cggatttcag gaaatttgct
     3421 gatctcttca gggaaaaggg aggccgcctg gcagtaatat cagtgtcgga agaacataaa
     3481 actaaaggca gcaacaattt tattcctgat atagaagtgg aaataacact tccctccttc
     3541 cccgatcctg tcaccctgag aaaacaaatt gccctgaaaa tgctacttaa tgcccattca
     3601 acaggtatta tggcaatact gggacggatt atcggcaata ccatgacaac ccttaatccc
     3661 agcaatctga agctcatcgg acgggctaca tatctgataa tgagtcatgt aaatgacact
     3721 atagcacaga aggaatggat aggcaagtat ggctaccatg agcccctctc tttcgatgaa
     3781 attaatgcag ttctctttga ttcaattgat tacatctcca gccgtgacgc cggggaaacc
     3841 gcggaggtcc ctctttccat aatccggatt cttgaatcac ttaaaacaaa aggtttcgtc
     3901 agctgggatg aaacccggca gattcttgac aggcagggtc tggaaaacta tctgatggaa
     3961 cataatccaa acctcacttc aggaaaataa taaaccaaca caagaagcat tatgaacttt
     4021 acaattattg atgccctgat tgtcatcgtg tacatggccg gcattacatt attcggaata
     4081 aagcttgccg gaaaacaaac ttcaaccagg gattattttc tgggaggacg aaagatttcc
     4141 tggtgggtcg tatgtttttc aatagtagcc acggaaacca gtacacttac atttatcagt
     4201 attcccggat tggcatatct cacaaatttc aatttccttc agcttacctt cggctatttc
     4261 ctcggaagaa taattgttgc ctggataatc ctgccggcct atttcaaggg cgaactggga
     4321 acagcctata cgtttctggc aaaccgtttc ggcacaccaa tgaaaaacct ggcttcaggc
     4381 gttttcatgt taacccggct ggctgccgac ggagtaaggc tttttgcaac agccataccc
     4441 cttgccctga tacttcaggg aacatttatc ggtaaaagtc tttctggtga ctggatatat
     4501 atctggtcaa taatcctgat tgcgcttgta acctacagct acacatttct cggaggattg
     4561 aaatccgtcg tctggatgga aatggtgcag ctttttattt atatcacagg ggcaattatt
     4621 gcgatagtta tcgtcagcag caagttaaca ggcggaatgc cggcaggaat tgaaacagca
     4681 gccgaagcag gaaaatacca gctgttcaat ttctcatttt cgggaggact caaaggattt
     4741 ttctccacac cctatacctt tatagccagc attctgggag gaatgttcct ttcaatggca
     4801 tcgcacggaa tagaccagct gatcatccag aggcttcttg caacgggaac tctgaaagcc
     4861 agccgcaaag ccctggtaac aaccggaggt atcatcatag cacagtttgc cctcttcctt
     4921 ttcctgggta ctctgcttta cgttttctac ggaggggccg atatcaggtc tgatgaaata
     4981 ttcccgaaat ttattataga agaaatacct acgggactgt cgggatttat cattgctgcc
     5041 ctgtttgctg ctgcaatgtc aactctttca agttccatca cggcactggg atcaaccacc
     5101 atgtacgact atgtaatgcc atactataaa aagctgaatc cggccnnnnn nnnnnatttc
     5161 cagaatgata acaggaatat ggtgcctcct gcttgtcgga tcagcagtct ctttcatgaa
     5221 aagttcccag gtagttgttg aacttgccct ttcaattgct tcgttcacat acgggggatt
     5281 gctgggtact ttcatactgg gagtcctttt caggaaaccg ctgataagac aggctgttcc
     5341 ggcttttctg acaggtattg cagttatggt ctacatcata atcttcacat ccattgcatg
     5401 gacctggtac accctgatag gaacgctggc tactgttctt accggactgg tcctgacctg
     5461 gattgctcag aacaggaaag caaatgaata agcggagtca acaccttgcc cggtctgcct
     5521 aacctgctta ttaagaatgc attcttaatc cttgtaccca gaacggtact gagaataaga
     5581 atacaacccg ctgaaccagg gtacattcct gatccctgta cgttgaacgg gactgaatat
     5641 taaatcccgg tacgccgggg ataggtggat tttaaaccag gaacttcctt acttcctcaa
     5701 gtatttcggg ggttccggca ttgaaccagt atcccgaatc ggaaagcagg gtatagatat
     5761 tcctttcggc agccagctgc aggtaaagtg gtgtcattga atacactcct tcagacatat
     5821 agctgaaaat ctccgggtct atcagtgcca tggatgaaaa cgcgatttca gacaggttct
     5881 cccctgaacc gcgtgaaata atcctttctc ccgtttgctt gttgcaccat cctctgagga
     5941 gtccttctga atctataagc agaaagcgtg tccccggcct gtgcctgaca accagtgttg
     6001 ccagtccccc tttctccagg tgataatcca gcagtattcc cagatcaaaa tcagttatga
     6061 tatcagcatt gtacaacaga aagggggttc tgtcaaaaaa atgacgggct ctgtacagac
     6121 ctccgccagt atcaaggagt tcttcccttt catccgagat acttattctg aaaccttccc
     6181 ttctcagccg tactatctcc tcttcaacca aaccggcaaa atgatggaca ttgattatga
     6241 tatcatcaaa tccgtgagac acacattttt ccacagcacg cctgaggaga ctctttccgt
     6301 ttatgtcaac aagaaccttg ggaatgcttt cagtgatctt tcccagtctg gttccttttc
     6361 cggcagagag aatcatagct ttcatattcc ggaaatcatt gtctttttgt ctcaagggtt
     6421 ttaaaaagag tggtgtctga ggttgcggca atggcacggt acagctccgg gagccggata
     6481 tgctgttctg cttttgctgc aatatcctga aggaggatca ctgcgggaac cagtgtctcc
     6541 gtaaaggtgg gttttttttc ataaagcccc ctgaaaccaa atgctcccag ggcctgcatg
     6601 agccgtatca tgctgaatcc tgaataatag cccctgaatt tctgcttatc ctcatttgtt
     6661 acagtacaga atttctcctg gaaataatct gccagttcgt cgcggttttc ctgtttcatc
     6721 ggaattttag catcaaaaag cagagatgcc aggtcatatt gaggggcgcc tttccttcct
     6781 ccctgataat caataaacca gggttcattg tctatcagca tgatattggc agtctgaaaa
     6841 tcccggtaaa ggaagtattt ctgacccgtt tccagaagat aatcggccag gttgttgaaa
     6901 tcctgctcca gggggcgttc ccggaaaggt actgccagca gtttcaggag catgtacttg
     6961 taataattca tatcccacat cattgactgg cggtcaaagc tcttatgcgg atagcacaga
     7021 tcgagtttca atccgtcaat tcccctggtc tgaaaaagta taagcctgtc aagtattttt
     7081 ttatacaact gctttgtctc atcattgaaa tccgagatat ccggcctggc gtgaagccag
     7141 gtatacagat tcaagtcgcc cagatctctc tggaaataga tgaatttttc gggcatataa
     7201 caataaacct caggaacagg cagctgcttg cggagaaaat gttcagtaaa accaacgaag
     7261 gcatcattct cctcacggtc tctgttgtat gttcctatca gggtcttttc cccgtcaaaa
     7321 atacggaaat acctcctgtc cgagcccgac cggggcaggg cttccgtttt agtcactgca
     7381 tttccgctat attccctgta cccttgcgat actttgctga tcacatccat taagtagtat
     7441 ttttatatca gtcagttttc atttttatgt cccacccccc gggaatctgt taccagcggc
     7501 ttccctttac acttagtttt cagccttgtc cgttctccgc aggttccgtt tcccgccgga
     7561 tctccggcat ttccgggaag tgcatcctgc caggcctgct ccttaccaat acgttgttct
     7621 gctgcaaaaa ttcacttctg catcacatta ttataaacgt atcataatct tttttctgta
     7681 taataccaat gcgataatcc agatgagcag aacctgcaaa atcgcaaaca taaggcttcc
     7741 attcatattg cctgcaacag gaacgcagat tttttcccat atccagttat aaaggttcat
     7801 ctctgatcca cctgcggtaa ccttgacgtt aagcattatt cttgtccaga tacctgcaag
     7861 gaaatatgaa aagagtgcat ttgtaccgaa aaccaggaaa aaagttcccc acttccgcaa
     7921 tttccacaca tctgcaatca ggtagatcaa tgcaaggacg cccattgcaa ttccggcagt
     7981 atagagaaca taggaactgg tccacaaagg cttgttgagc gggaagaata tactccagag
     8041 aagtccgagt ccggtcgagg aggctcccag caacagaagc ttcactacct tatcccagcc
     8101 tgcagaaccc ttccccagca gttctcccgc gtaataaccg atcaggaccg tggccactgc
     8161 aggcaaagtg ctgaaaaggc cttccgggtc gaaagggatg ccatagcccc tgtacaagtg
     8221 tttttctccc agtatggcga tatcaaccct gagagcaaaa ttatccttca gactgtaggg
     8281 atcagtaccg cctgcaaaag caaggaccgc ccagtatgag agaagaatta cggcaatcac
     8341 gacccacagg taattcctgt tgacagaaag acatattatg gatcccaggc agtatgccag
     8401 tgcaattcgc tggagcacac ccattatcct cagactggaa taatctctca cgaaatgagg
     8461 gaaaattgta aggaagatcc caagtgcgaa gatggtcact gtccgcctga atatccttag
     8521 caatgagcca ctgttcaatt cctgaccgta ttttttcatt gagaaccaaa gagatactcc
     8581 aacgataaac aggaagaacg gaaaaaccag gtctgtcgga gtgcatccat gccaggcggc
     8641 atgacgcaga ggaggataaa cataatccca gctgccggga gtatttacta ttatcatgaa
     8701 ggctattgtc attccccgga aaatatcaag agaaaccagc ctgccgctac tttttgccat
     8761 tattaactgt tctgggatta atttcagagt tctattatgc caattccggg tctgtctttg
     8821 agaatgatcc tgccgtcttc cacagtgacg ccgtcatata tatcattact tatcagcaaa
     8881 ttgccgtcca gatcggccca gtcgaccaga ggagaaaact gtgcagccgc agaaatggca
     8941 caggatgttt cagtcatgca gccaaccata attttcattc ccattgccct ggccatcaaa
     9001 gccagcttat atccggcatt cataccaccg catttcatca gcttgatatt gattccgtca
     9061 taaaaatcct ttaccctgaa caggtcatcc tttgtctgaa atgcttcatc cgcaatcacg
     9121 ggaagaggtg aattctgagt gagccatccc gtttcttcag tcatggtttt gggcattggc
     9181 tgttcaagat atataacatt ctgttccttg agccagtgcg ccatttcaag ggcataattc
     9241 ctgtccttcc atccctgatt gacatcaaca ccaataggtt tgtcagatac tgaacgtatg
     9301 gtttcaatta tatttttatc attgccgaca cccagtttga ctttcagtat tttataaggt
     9361 tcagcctctg caaccttctc tcttacaaca tctgctgtgt catcaccgat ggtgaaactt
     9421 gtacagggtg ttttttcagg attcagtccc cagatcctgt accatggctg acccattatc
     9481 ttgccagcca ggtcatgaag ggcaatgtct attgatgcct tggcagcata gttgccggga
     9541 gcgattccgt caacatattc aagaatatcc tctatcagga aggggtcgct gaactgttcc
     9601 atgttaaccc gggaaaggaa tttctgaaca ctttcatgac tttctcccag gtacggaggc
     9661 attgatgctt caccgtaacc aacaaagcca tcacattcca gcttagtcag cactacggga
     9721 gttgtggttc tcgatccagc agcaagtgta aatacatgct tcagctgaag tgtgtacggg
     9781 gtgtatgtca gttttatagg tgaagttttc cgggatctta ccccggtttt tcctccggaa
     9841 ttatttccac aggacgtaaa tgtgctgctt atcattgcac ctgtaagcaa tcctccatac
     9901 ttgataaaat cacgtttttt catctgtacc agttattttg tgaaatatgt tccagaccct
     9961 ttcctgaggg gactccgatt atccttcttg caccctgcag aatgctgacc aggtatctgt
    10021 cataatctcc ggcgtcagga tccagaatgt tgttcgccac cattcccctg cccgttgagg
    10081 catgaataaa ttcattgtta ccgatataca tgcctgtatg tgttattctt ttttccccct
    10141 tatcgttcac cgatccgaaa aacagcaggt ctccgggccg gagtgcttca aaggaggatg
    10201 atatatccac ttcctctccg taacggaact gctgggatgc atcccttgca aggattatac
    10261 ctcccgtaaa ataggctgtt ttcaccagcc cgctgcagtc gagcatcttt gttgacgttc
    10321 ccccccataa atatgaaact ccggtcaggg attccgcaaa actgacaagc ttatcagctt
    10381 caggccttac agtctgacgc cacttctcaa aatcctccgc atccttccgg tttattcgtc
    10441 cttttctgcc atcgggaagc tcaactgtaa agtaatccct gccggcagat atccttttca
    10501 gaatagaacc cgtcaccaca tccgatacaa cttcctttcc gcttccttcc gaaagtatat
    10561 cccccgttct tgaagtatag ataaggcggt ccgattcctt ccattcctga aatccgcttt
    10621 catccattcc actgacactg ctggcggtag tccatcccag gtaatgatcc ggtgtctgca
    10681 tgagcaacca gcttccctcc tttttgagga tccttaccgg agtccccatg acagtctggg
    10741 ataccagctc gcttgaatgt gacggatcct ttttcagatt tgcaacggaa acgcatatta
    10801 aaccccatga tctggctgtt tcagatttgt cgggaaggac tttgatactg tcagcatatc
    10861 cggtacccga ctgttcaaga tgtttaagga tctctgcctt gagttctgga atgctggttt
    10921 ctccctttat caggagcctg cggtctttca gtatctccat gtccagattg caaagggctt
    10981 ctcgcttatc tggaatatac tgaagcacga tggaatctat ttcattctga aggcttaacg
    11041 gaacctctga cggtttgcat cctgaaatga aaagaagaat caataaaatc ctgaagagat
    11101 gtttcatagg ttttcaaaat ggtaaatatc atgcaaactt attaaattga aaataaagat
    11161 tctgaataaa ttgtaaaaat tagtcaacaa ttacagatca ggaagtaata ataaactccc
    11221 cgccgccggc ggatatccac aaaagtatcc tatacagcag cttccccgga ttatttatca
    11281 caatttattt tagttacttt tataaattaa acttataaaa tataattcat gtttgccttg
    11341 agaattgttc tgatagcgct tatacttggc tttcaatggg aggatggtat gaactcccgg
    11401 gctgtggaaa ccggtctgga agttctcacc ggggatgatt tcagcatcct gaaaggaaaa
    11461 agagtcggac tgattacaaa tccgaccggt gttgacagaa atctccggtc ggcagccgat
    11521 attctgcaca atgctcccgg ggtaaggctc actgcccttt tcgggcctga acatggcgtg
    11581 cgcggtgaat ttgcagctgg agaaacaatc ggaccggcag tggacccggc aacgggaatt
    11641 cccgtgtaca gcctttacgg gaaaacaaga aaaccgactc cggagatgct ccgcaacatt
    11701 gacgtacttg tttatgacat tcaggatatc ggctccaggt catatacata cataagcaca
    11761 ctggggctgg ccatggaagc agccgcagag aacaatatac cctttctggt tcttgacagg
    11821 ccgaatccgc ttggagggat caggatggaa ggacctctcg tacagcccgg attcacatcg
    11881 tttgtcagcc agtatcccat accctatatt catggcctta cagtggggga actggctgag
    11941 tttcttaacg gggaaggtct gcttgaaaac ggtgtaaaat gcaggcttga ggtggtgaaa
    12001 atgaaaaact ggaaaaggga tatgctgttt gaggatacag gtctgccctg ggtgccttca
    12061 tcgcctcaca tcccgaatgc acagacagcc ctgttttacc ctgcaaccgg tattctcggg
    12121 gaattatata ctgtcagcat cggtgtgggc tataccatgc ctttccagct tcttgcagcc
    12181 gaatggatcg atgctgattc acttgctgca gcactgaacg cactttccct tccgggggta
    12241 agattccgtc ccctgcatta caaaccatac tacagtgtgg cacagggcac aatggtgcat
    12301 ggggtgcaga tacatcttac tgatcccgcg aaagctcccc ttaccctgct acagttctat
    12361 ttccttcagg aagtccgtaa actgcggcct gacaaggaca ttttcagttt atgcgatccg
    12421 tcacggctgg atatgttcga taaggtgtgc gggactgaca gggtgaggaa ggaatttctt
    12481 aaaacctggc aggtaagttc tgttctggat atctggaacg gagacattcc gcaattcagg
    12541 aaaaaggcag agaaatatct gctttataaa taaacagaat gaacagcgac agcctgttga
    12601 ttcaatgcca ttccggactg tttccctctg tgcttttctc cagctcgcgg gcatgtacca
    12661 ggaattcatc aatactcatt tccctgtaaa aaatcatacc gtgagtggcc cgtatcttag
    12721 gactttcatt atcatagaaa aagtcctttc ccagtatcag ctgaaccctc ttaaactgtt
    12781 caacccatat ctgcatcgac aggtcgctga agggcccgtc aagctcataa ctcggaaagg
    12841 aggcatcaac ctggctgata tagcagttcc tgaatgacca gtcaagcaca gccattgaat
    12901 taagcgacac gggagtgaat acattggctt cggatggatg aaaacggaac catacattcc
    12961 tgtagcctat gattctcaga tccttcaggt ctttttcctt tttccactga atgagggcat
    13021 gtgctatggc ctggaggacc ttgtaatgag tgtcagggcc tgatccttca ggatcaaagg
    13081 caagacttat cacagtaggc tgtattttcc tgaacatttt cagtatcggc atcacatccc
    13141 ttgttttctg gggctgggat gtgaatatat ctccggtata gaatccaagc cgcagatgct
    13201 caatgttttt gactctgaca ccataatgtg cccacacgag ttcttcttca aactccctga
    13261 gcattccttt cagtttctga atgtcaggag gattcttttc cccgctgtaa ctgtttcgca
    13321 gagtctccac attcgcctgt aactggttca ccaggtcttc cctggactta aggttgtata
    13381 taccgaccat tactcttgtt atccggtggc aaacgccccg gagtttaccc ttttcatcac
    13441 ggtcggcaac cttgttcaga taatggttta catccttgtc aaacttgtag cggtatccat
    13501 tttcgaaaaa atcgggatag gcaagcatct gtatctttcc ctgttctata tgttccaggg
    13561 tttcaaccag cagattttca agcatctcat tcgttaccgc ggtaaaccct gatgtaagaa
    13621 cggcaaaatg gaatttgttg gaacgattgc gcagctgtga aatgatgctg ggaaatatac
    13681 cgagcattat atcatcatgg tgaggtccgg tatggaggaa aacctgatca ttctccctta
    13741 tgctccccct ttcaatcttc ctttttgttg atatgatcac atcctgtacg gctttgagac
    13801 tcagaccggg aataagggaa cagtatttat cgttcctgag atcatccaat tccagattgt
    13861 gtgcatattt atctatcttc cggcacaggt cgtatactgc tctttccgtc ttttcgaaag
    13921 tccagtcccc ctgccggtag taactttcaa tactgtcgtg aagctgtact gccgcacccc
    13981 tggtgagata gaaacgtgcg ttttccagtc tctgcagtac agttgccgga taaaggttgt
    14041 ccggctcatt ttcaagggca tttttaataa tatgggcctt ggactcacct gccgcaaaaa
    14101 ttattgaagt gcattcggga ttgtaggtca gggttcccag accaatggtc agaaccagcc
    14161 tgttccttga tatctcgatg cctcccagat ctcctgcggc agcggcctgt gattcgaaat
    14221 tggtagccgt tagcctcgtg gttgaattat gatcacttcc ccgggaattg aatgcaatac
    14281 tcccgtcagg accaattcct ccgagaaaaa aaccaatgcc tcctgtttcc ctgatacggt
    14341 tctcatattc catgcaccat ttatctattc tgaaaagtga agcctgctga attttttcgt
    14401 gaggcgtcag agcttccctg ttccggaggg aaagatctat ccggttgccg ggaaacaccc
    14461 gggaataatg cattccttca gccagcctga tctcatcgga attcataagg agggcagcgg
    14521 caggatcaag gccaaatccc tttatataga aattaagaac ataattgtaa aaactgttat
    14581 gctggcccgg atcaatcgga taaaattcat ccatctgaac aaagcgcagc tttttcataa
    14641 caggtttctt cctgatggtg aagccgttct cttccattat cctcctgttc tcaggcctgt
    14701 tccaaccttc aaggaatctc cgggtccatt tcaggaaata ttccggcgtc ttgcctgtcg
    14761 gcagactgat cagcccttca ggattctccg aaacccattc taggaacctc agggaggtaa
    14821 gcaaaccgag ggcagggaaa ttatccacca ccaggtaagg tatctttgtc gaaaaccccg
    14881 ttcttcccga acgcttctga aatgtcagct caacattcgt catgtcttga atttcaataa
    14941 attattgttc caaaccagtc attcctgcaa aacgaaaagt atggaaattt ataaatctga
    15001 tattttcatc cttccggaag ctgtccggta actgcaaaca ttacaatgaa catacccgaa
    15061 cagtggcttt acctgaagtt tctgtacagt gatagcggga atgcactgtc actttgacca
    15121 tatactgaca gcaggtttcc cggctgccgg ctacaggcag cagttattac ctcatcaggg
    15181 catcatacat tatttccacc ggatgaaccg ccttctttcc tgttccgtca tagatctggc
    15241 atctgcagga tgttccggga gcggcaatta cagtttcttc cgatgcagcc ctcactgcag
    15301 ggaaaagcac cagctcccct attttcatgc tgagttcatg atgttccttt tcatatccga
    15361 atgatccggc cattccgcag catccgctgg gtatctcctc aatctcataa ttctcgggaa
    15421 ttgacagcat tttccttgta aaggaagtag aagcaatggc cttttgctga caatggccat
    15481 gcagtttcac agaaagcgga tcatgaacaa actgttcggg agtaattttc cccttgtcat
    15541 attccctgca aatgaattcc tcgatcataa tggcattttt cccgagtttc accgcggcat
    15601 catggagttc cccgctaagg agatcgggat attcatcccg gaaagtcaga attgctgatg
    15661 gttctatacc gacaagcggt gttttctccg tcactatatc cttcagcatc ctgacgttct
    15721 tctcagcaat tttcctggca cttttcaaca gacccttgga caggaatgtc ctgccgcttt
    15781 cggcatgtgc aggaacctca acctggtatc ccagtgtttc aaggagaata attgccttga
    15841 ttcctatatc gctttcattg taattggtga attcatctgc aaaaaggaac accttgccca
    15901 agggattctt catatgcatt gaagtggtct gacacttttc agcccaggtc ctgagggtga
    15961 tcttggaaag ggaaggaagg gtcctgtcag gattgaaacc aatcagtttc ttgaagaagg
    16021 actggcgcat gataaaattt gtaaggggcc atatcgcatg accaaacctg tttatcctgg
    16081 gcagccatcc tatgagcagc gtccgtatgg gaattccgtg cagtctgtaa tagtggctca
    16141 ggaattctgc cttgagttta gtcatatcca cgtttgacgg acattccgac ttgcaggcct
    16201 tgcaggaaag acaaagatcc atcacctgcc atatctccct gtgattatag gtatgcttct
    16261 tcgtggaacg cgtaaggaat tccctgagta tgttggctct tcccctggtt gttttgtctt
    16321 catcttccgt tgccataaat gtcggacaca ttgttccgcc aatcagcgga ctcttccggc
    16381 agtcacccga tccgctgcac ttttctgcag cactcagaat ggtacccttg tcggggaaga
    16441 cgaatgttga atcaaggggc tcgatatgct caccggtccg gtatctcaga cttgtatgca
    16501 tcgggggtac gtcaacgatc ttgcccggat tgaaaatatt ttccggatcc caggttcttt
    16561 tcagtttcct caggatgtta tagatatgat ctccgagcat caggggaatg aattcccccc
    16621 ttaatcttcc gtcgccgtgt tcaccgctga gggaacctct gtatttcttt acgagctgag
    16681 ccacatccct tgccaggttc cggtatatat caacatctgc cggatctttc aggtcgagca
    16741 gggggctgag gtggagctcc cccgttgcta tatgggcata ataggcacag ttcagactgt
    16801 atttcttcag aatctgctgg aattcttcaa tatattcagg aaaatattca ggaagaacgg
    16861 ccgtatcctc aatcacctga acactgcgtt tctcccccgg aatgatcaga agaaccccca
    16921 ggcccgcttt tctcaaatcc cagattcttt ttatttcatc ttcaccggtg aacaggggaa
    16981 aatggtatcc gtagccggct gcggtcagtt ccttttcaat gtcggaagca catttcttca
    17041 gttccttttc agtgtttttc agtaactcga taagaagtat gattttagga cttcccttga
    17101 taaaaaacct gtttttgttc tgttcaatat tatcctttgt acattccaca ataaagtcat
    17161 cgatcatctc caccgcggcc ggttcatgtt tcagaatcag attatttgcc ctgacagcct
    17221 catccaggct catgaaatgc gcagtgatca gtcctgtttt ccggctttct accggaataa
    17281 ggttgagctt catccttgtg gtaaaagcaa gggtcccctc cgatcctgcc agcagtctgc
    17341 acaggttgat cttttcgcca ttgccggaaa acggatccgt ttcaagcagg gaatcaagtg
    17401 catagccatt attcctgcgt tttactccgg gatggggata ctgcgcacgt atttcatccc
    17461 ggttcactga atcggacagg atatctttta tgaatctgta gattgagcgt tcaagtgaat
    17521 ctttttctcc ggaacatttt tcatcgaatt ccttcctcga aagagccctg aatgttgttt
    17581 cggttccgtc cgacaaaata acatccgcct cgagaatatg atcccttaca cttccgtaga
    17641 tcagggaatg tgccccgcag gaattgttgc ccagcattcc gcccagcgta caccagtctg
    17701 ccgtggatgt ttcaggtccg aacatcaggc cgtggggagc aagcttcctg ttaagggctg
    17761 taaggaccac accgggttca acaaccgccc atctttcctc aggatttatc tcaataatcc
    17821 tgttcatgta ctttgaaaca tcgataacga tacctggacc cacaacctgt ccggcaagtg
    17881 atgtacccgc ccctcttgga atgacggaac cctttatggc cctggcaaaa gcaataacct
    17941 tccttatatc atccttgtct ttcggccgta caacggctct gggcagttcc ctgtacgccg
    18001 aaccatcggt ggcatacagt atcctgtgct tcctgtctgt gaacagatct cctttcagtc
    18061 cggccctaag tgaatccagt ttcattttat tcattctttt ttatcccgaa ggaagggtca
    18121 attttaagaa atttaataac aaccattccc ggaatcgtac aaattaaaac ataaataaag
    18181 aagtgctgat agccgagcag ttcctgaact tttccggaaa ccattcccgg aagcatcatg
    18241 cccagtgcca tcagagatgt ggctatcgcg tactctgcag ttttataacg gctctcaccc
    18301 acaaaatata gcatgaaaag catgtatgca acaaatccga atccgtatcc gaactgctcg
    18361 agcgcaattg caaggtaaat gcctgggttt ccgggcatcg gctgaacgta tgcaagatat
    18421 atataaacgg catcaggcag gtttatggaa attgccatta tccagataag ccgtttcagt
    18481 ccgtattttg ccgctgctat tcctcccagt atccctccta tcgtcaggca gagcatacct
    18541 atggttccgt aggcaatacc gtattccgtg gatgtaagtc cgatacctcc ggagctcctg
    18601 gaatccacaa gaaagggtgt tgcaaccttc accagctggg attcaccaag gcggtaaaaa
    18661 agaaggaaaa taagcgcggg gatgataccg ggttttgtga aaaaggaaac aaaaacttcc
    18721 ttgtatactt tccagcctat cttttccctg ttatcgttct caggggggta aggcagcagt
    18781 atcctgttgt agacagccag aagcaacaaa atcagcccta ttatgcccag ggctactgtc
    18841 cagctgaaag caatatttcc ggcggtaaca agaaatcccg cggaagttct ttcatcaagg
    18901 ttcctgttga ttttcagggt aagggatacc ggtttgtccc agtttgaatc gctgaattca
    18961 taccttccgg tctggtttgc agcaatatca atatccttgc tgccagtgtt cctggaggcg
    19021 tttacaacta cggtttttcc atggccgggc ggagcagaca gggcaatgta gacaactgcc
    19081 gaatccacct cagactcgcc tgtcctgaac atcggtatct gcaggtttcg cgggaaaaga
    19141 aggatggcag gctctcctgc tgattcctca atgacaattg cagagggatc aaatggtaca
    19201 tattcatcag ccggtaccga aacagcctca aatgtaacag attccagccc cgtatttttc
    19261 tgaaccactc ctgcaatgac aggaaccagt ccgagggctg tcagcattgc gaaacggtaa
    19321 aatgttgacc ggatgcccac aaaaaaggat tgctcgtgct ggtcgagggc atacatgtaa
    19381 tatccgtcgg cagctatatc atggcttgcg gaagcaaagg caatgattgc aaagattccg
    19441 agtgtaacgg gataaaagaa ggaaacaggc attgcaatgc cggctccgat aaatgcaata
    19501 ccgagaagaa gctgtgtcca taccacccag ttccgtttgg tggaaacaac atcgatataa
    19561 gggctccaca ggggtttcac tgcccagggc agataaagca gactggtcca gaatgcaagt
    19621 gaagcaatgg aaatacccat ggttttgtat ataagaccgg aagtgatcat tacaatacta
    19681 tagggtacac cctgaaaaaa ataaagggtt ggcacccatg cccatggcga acggtaggct
    19741 tttcgttcca taatacggct ttataagctt ttaaatatat atccttttgc cgaaaagaaa
    19801 gtgattattt ctccaagctt taaatataac ttgtcagtcc ggtcggggtg ggtacccgga
    19861 tgaatcagga tcattgcccc cctgagtccc cggggatcag aggcttcata tcttttaaga
    19921 ccctcaagca gttcatccga cgacctgtaa ttactcatgt ccggtgttgt ataatctgca
    19981 tttgtcccgg tgccgggagt gaaattgata agtttgatcc cgagggagga agtccagtgt
    20041 gcaatggcgg aattatacca ttcatatggc ggaaggaagt ataatgtccc tggtttgcct
    20101 gcctttcttt ttgccagctc cctgtaattt gccctgaggt cgctgacaaa ctgctccctg
    20161 tcaaccagca gtgaatctct cttttcccac ggggcataaa gcagatgcct gtcggaatga
    20221 ggaccggcaa aatggccacc ggatacgata cggcgtatat atttctcatt cccgggattt
    20281 ctgaggaaat ttcctgtaag gaaaaaagag gcaccggatc cagttttgga aagaacatcc
    20341 agaatatgat caaacccgtc attgtgatca tcagctgaaa aaagcagata tatttctttt
    20401 ttgttgtttt tccggattat tgcgccctgg tcatcaatca cttcctctcc cggtcttcct
    20461 gtctgccgga gaccctcact ctcgagagcc gacatgtata ttgtaagatc tgccgtgcca
    20521 tccatggtag gctcatttga actgtagtca ccggtgtcat catgatagat tgccttcccg
    20581 ttctggaagg gagcataggg atcgggccgt gtgagggtca gtcctatctg ctgatcatat
    20641 attctcctgt aaatgggtcc gtcaacaagt cccccgggcg gtattattcc tgaaaggaca
    20701 gtcaccgaag catgaggata gcgcggatag tcacctcctt ccggaagtcc ggttatcatt
    20761 gaagtgcccc agggattaca gccaaacagc cagtcgcggt gagcggcttc cgtttcagca
    20821 aaacggctgt cgcccgacac ttcggcataa atcctgtact gggtaagggc ggccgtcaca
    20881 taattgtttg aacaccatat gaagggtatc tttattctga aaggatcatt ctttttacgg
    20941 tcttccagaa tttgcaggcc cctcctcatg aatgaaatga actttccgga atatcttgtt
    21001 ccggattttg ccagaccggc atggccgagg tttacaaagg gataaaactg gtaatgacgg
    21061 gcagtatcca tttctatcca cggtgagatt ctctcgagtg ttccccagta atcggcctgg
    21121 accagatagg cggaatcccc tgtcaggctg aacagttcaa ttgctgccag ttccaggtca
    21181 tccacataat tatcctcttc atagaaatat ggtgatacca cgcaggctgt ctgtgtcgtc
    21241 ccaaggtccg taagcccgaa cctccaggct tcttcagcct ttttccgcag ccggtccgca
    21301 agtccgctgt caatatccct gaacacctcc gccccgatgg caaatgccga tgcgaatttc
    21361 cctgctgtgg aagaaacacc cgtgctcctg ttactgtacc gggaaagccc ctgtgacttt
    21421 cctgtcacaa aataaaccgg ccggtgaact cccaggccat aggaagcctt gtcaccggcg
    21481 ggcagcctga atccggcatg atcccggtca tcggcaatct gattgtacat ctcgccgctg
    21541 tctggattca ttttaagaag ccagtcaata ccccaccggg cctcgtccag aatgtcggga
    21601 ataccgttgc tcccgggatt tcccgaggca tcatggctgt cgccgaacac ctccggattt
    21661 ctcctgtagg cataaagcat ctgatagacg gcattagccg aggtgcatgt gtactgaagg
    21721 taatccgtgg catcatgcca tccgccggta acatctatgc gctgcccaga tttctccgga
    21781 tggtcaacaa tgatagcatc atgtacatgg caggaatcac cataaaaggg attgtatccg
    21841 catctctgct gccgcatgta ttccagcagg aaatcactcg ttcccctgta cacatccgca
    21901 gaaatgctga aagcttcaga ttcggcgttt ccgaagcgta taaaatagcc cccgggagtt
    21961 tccagttttg aaaaatcaag tcgtgctgct gaacgcatac cccactcttc tccggaatag
    22021 tgccgggcct tgtctttgaa aacaacagtt cctgtgatgg cattatgaac tgtaaaatat
    22081 ttatcagaaa ggccgtcggt tgaaatacaa accgcaacct ttactgactg aggcagatag
    22141 cccagctgat tgatcctgat ccaggactga cctgaaaggg tgccggccag aaataaacct
    22201 atcagtatta tgatgcttct cttgttcatg atttatgttt taaaattctt cgaacccatt
    22261 attcacagcc ggtcatgaaa attattaccg tccgattctc cgttcagtat attcttttca
    22321 gttcagtata ttcttttcaa ttcagtatat tctttttagt tctgctcccc tgaaatagtg
    22381 cataaggatt tcatcatagc ggtatccctc tgatgccatt actgccgcac caatctggca
    22441 cagacccaca ccatggcccc agcctgcacc atgaaggatg aattttgttt ctttcccgct
    22501 ctcagttttt tccacataaa agcaggaact gtaaagatgg cttttcgaca atgatttcct
    22561 gatttccagt tcctttccga ttataagatt tttcttcgtt ccctcaatct tcaaccttgt
    22621 tatccttccc gaactgcctc tttcgagggg taccaggccg gtcaccgttc caaaatcaat
    22681 acctgttctt gtcctgatga tgtcactgag ctcatcctgg ccgtatgtca ccttccacct
    22741 gaaaaagtcc atggtttcca tatcatagtc attgagtacc tggcccagta ttttcttgtc
    22801 ggttgtattg cagaatgctt caggactgct ccgtatccag tcctctgccc tttcgttatc
    22861 caccggtgcc tgactgcttc cgggaggaga agaggcatta tccgtgatgc tttggagata
    22921 cggatgatgc accggtcccc aaacgttttc aaacaactcc gtgattcccc cgcagcattt
    22981 ggagaatctc gtatcacaaa tttcgtcacc gtacacaagc acttccccta tggtctcctc
    23041 aaccgcttcc gcaacaacag gtgaggccgc gcgggtaatc ccctggtatc tctggcaatg
    23101 gtcatccgca cagacatcat agttcgtgtg atcctccctg tcgtaccacc ttgttatttc
    23161 cgtatcgctg atgaactctg tccggaccgg actgttctca cccctgttct tgcggctttt
    23221 ctcgatttgt gccaggagcc agcttctgga aatcactgca tgggccttca gaagctctgc
    23281 tgatgaagtt gccttcattt cggaggagat tacgcttttc agatattcct caatatgtat
    23341 cagattaacg gctgtgatcc tgtcttcttc ggcaataaac ttcagacttc ctttaaaaac
    23401 ctgatcctct gttctttccc agtggaatcc tatacctatc accacatcat gcagggtaaa
    23461 ggacatatca gaacctgccg gtttgagaat gatctgcccg gtcgagctta tttccctttc
    23521 ttcagaaacc agaacaattt tgccattacg gtaaaatgcc ctgcacttat ctcccagcac
    23581 aaggttaccc ggatcggatt catatttccc gttcagagta aaggaaatct cccgggctga
    23641 caatattccg acatgaagtt cctgtatttt atcctggctg ctcattttat tgttttattc
    23701 attcagtatc cggctgtttt atccatttag catctgattg ttttaaccaa ttagcatccg
    23761 gttgctttgc ccatctgaca tctgatcgtt ttatcctttc agcattctgt acaattcatc
    23821 ttctcccacg cacacctgtc tgaaaatatc tgaaatatca ttttcagtaa gcttgtcaaa
    23881 atcctcctcc cggggagtaa ttaccagtcc gccaagatcg accgaagcag gactgattgt
    23941 aattttatca ctgccggcag caaaaaactg cccagggcgg tgctttttcc gcgggattat
    24001 atgaacaata aggttctccg tgttccggta tacaagtatg ttcatcatag gttccatctc
    24061 cgcattccgg gactggtctg caggattccc tgtgcccgga agccgggaat catccccggg
    24121 gctccggaac tctgtagtat gctctggctg atcccggagt gagagttctg atccttgtcc
    24181 cgatcctgct ctgtttaccg tgagaaggga gagcttttca taaacagatt caaacaggtt
    24241 ttcaaggatc tccttttccc tgcctttcag ggtgacgatc cctctcagat agcctgtcca
    24301 gtacaatatt tcgtaccctg gtcctgtaga taccacatgc gtaaaccgtt gtgattcaaa
    24361 gtctttctcg agaggcatga atcctctgct gactgcctgg aaatgaaggt ggtcaggggc
    24421 tgaagcaccg cattgcggac cattgtaaaa aacaacataa tcaggcaggg cagctgcaat
    24481 ggaaagcatt ttgccgaaat tattcattat cctctgttcc acgtgactgt ttgaaacgat
    24541 ggtcagatgc ctccggatta tcggataggg attaagcagt acggtaaact gatcatcaaa
    24601 ggggagtcct ctttgctgcg gaggcctgtt cctttcacac aggaagcagg gtcttgcctc
    24661 aatggaacct gaatctgtct ttgcagcggt cgatctgtat ctttcgggat tgaactggac
    24721 cagcacctca aaaccaccga aatcaagatg ccgtatttta accttttcca gctgacggaa
    24781 attcctggct gccagctccc attcacccag ctgggccagg aaaaattctg atactctttt
    24841 cgaatatgta ttactgtgca ctcgcctgaa gactatttat tctgatcgtt gttttaactg
    24901 tttaataatt gttgtctgat ccctgttttg ccgttgtaat gagattcgtt gccttaaccg
    24961 ttttattgtc cggttgattc ttgttttacc gctgtaacga tacttttctt tttcttgcca
    25021 tcagttccat tgtcctgatt ctgtccttgt acaggttatt tgcatttgtt cttactatat
    25081 cgagtgccgc atcggtattc ccttcccatc ttctgcacag atataatgat tcatatattc
    25141 ttccaatctg ataattacgg cttatagcca gtcctgctgc ataatcctcg ccatagctca
    25201 catcagggat gccgatactg cggattaccg gtgtataaaa agctctcggt gctcccagtc
    25261 cgttgatccg ca
//
LOCUS       DAOSPX010000010        23161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2387_length_23161_cov_4.648576, whole genome shotgun sequence.
ACCESSION   DAOSPX010000010 DAOSPX010000000
VERSION     DAOSPX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23161
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2387_length_23161_cov_4.648576"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLZ75_01100"
     CDS             complement(<1..401)
                     /locus_tag="PLZ75_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOS71026.1"
                     /translation="MAVKIRLARKGRKKLAHYHIVVADSRSPRDGRYIEKIGSYNPIT
                     NPATIELDFDKALGWLQKGALPTETCRAILSYKGVLIRKHLLEGVRKGAFDEAEADRR
                     FEEWMKQNEAKIDAKKSGLEKTKETEIAKKLA"
     gene            545..4030
                     /gene="dnaE"
                     /locus_tag="PLZ75_01105"
     CDS             545..4030
                     /gene="dnaE"
                     /locus_tag="PLZ75_01105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOS71027.1"
                     /translation="MAIFSHLHVHTQYSILDGASSIPLLVDRVKSLGMEAVAITDHGN
                     MFGVKEFHNAASRKGIKPIIGCEVYVARRSISETPGKEDRSGDHLILLAKNLKGYRNL
                     IKLISLSWIKGFYYTPRIDKELLAEYSEGLIACSACIAGEIPDKILNVSIEAAEEALK
                     SYISIFGKDFYLEVQRHETHEPDADPTVFPKQQRVIEAVKKLAARHNVGIIATNDVHF
                     VNAGDAEAHDRLICINTAKDIDDPDRLRYTKQEYLKSEEEMRRIFADMPEAVDNVAEV
                     VGKIEKYKLDHDPIMPDFDLPAGFEGKDEYLRHLTYEGARERWGSPNSEQTERIDFEL
                     QTIAKMGFPGYFLIVWDFLKAAREMGVSVGPGRGSAAGSAVAYCLKITDIDPIEYGLL
                     FERFLNLDRTSMPDIDIDFDEDGRESVLNYVVDKYGHDRVAHIITFGSMAAKMAIRDV
                     ARVQKLPLADADRLAKLVPEKPGITLLQAYQEVPELAKERESSNTLVSQTLKYAEVLE
                     GSVRQTGVHACGVIIGRESLDNYIPLSTAKDTRLYVTQYDGNHVQAVGLLKMDFLGLK
                     TLSIIKDAIRNIKNSRGIDLDIDSIPLDDAKTYELFSNGETTAIFQFESAGMKKYLKE
                     LMPNRFGDLIAMNALYRPGPMDYIREYIRRKNAGGKVEYKLPVMEKYLSDTYGITVYQ
                     EQVMLLSQELAGFTKGEADNLRKAMGKKKRSIMDEMKVKFFEGSLKKGHDEQTISEIW
                     NDWVSFAEYAFNKSHSTCYAYVAYQTGYLKAHYPAEFMAAVLSRNISDIKKITTFMDE
                     TRRMGMEVLGPDVNESYVNFTVNKDGNIRFGLGAIKGVGENAVMQLIGEREKNGLFRD
                     IYDLVERVNLNSFNKKNLEAMAVAGAFDCFEDMSRAQYFALDNRDSSFIESLIRYGQS
                     AKAIRQNNSQTLFGESGGFDLVRPLPAACSDWPKLEKLNREKEVIGIYLSSHPLDDFR
                     LEINTFTTATLADLQNLRNYLDREVTVAGMVTDTRNGIGKNNKPYGSLTLQDYTDSFR
                     FMLFDRDYIDNSKFFTIGYYLLVRGKVRKRKYREDELEFSISQISLLSSVKDELIKSI
                     TLKIDPVNIDGEMIRELRQLVSDNKGETEMRFMFLDTEDRISLPMFSRTLRVRLNNDF
                     ISYLEDHPSIDFKVN"
     gene            4067..4384
                     /gene="trxA"
                     /locus_tag="PLZ75_01110"
     CDS             4067..4384
                     /gene="trxA"
                     /locus_tag="PLZ75_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOS71028.1"
                     /translation="MAIEVSDGNFEEVVIQSEIPVIVDFWAEWCGPCRMISPILEEIS
                     KEYTGKALVTKCDVDNSPRTTAKYGIRNIPTVLFFKKGEVADKQVGAVPKSNFVNKLN
                     AIL"
     gene            4420..4998
                     /locus_tag="PLZ75_01115"
     CDS             4420..4998
                     /locus_tag="PLZ75_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOS71029.1"
                     /translation="MMTVCVFAASSSKFENEYGKDASRLGSLLAKAGISVVYGGGGIG
                     LMNALASAVISNNGNITGVIPAFMQAEGWGNDGENRTIVTSDMNERKRKMFGLSDAIV
                     ALPGGMGTLEELTEAITLKQLGLFNGPVIILNTCGFYDYFLKFTDNMVENNFLRKEHK
                     GMWEVVNSPEEVMEALSRKAGKNRTWRSIARI"
     gene            complement(5003..6094)
                     /gene="ribD"
                     /locus_tag="PLZ75_01120"
     CDS             complement(5003..6094)
                     /gene="ribD"
                     /locus_tag="PLZ75_01120"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOS71030.1"
                     /translation="MPASEDIKFMRRCIDLAAGAEGNTYPNPLVGSVIVSQGRITGEG
                     FHVRAGEAHAERVAVNSVISRGLPEDSVLYTNLEPCVHHGKTPPCTDLIIETGIKRIV
                     AGTIDTSDRVGGRGVAALRNAGCEVITGVLEEECRWLNRRFFTFHEKKRPYVILKWAE
                     SADSFLDVERDIYNGRRRPTWITGKPERVLVHRWRAAEQSVLVGAGTLRADDSLLNVR
                     DWAGNDPLKLILSSSGRLDKDSGIFHTGGSVTVFTHDPEAAGGYPAVIVPLERGLSSA
                     VQILSHLHDSGIQSLFIEGGAEVLGMFISGGLWDEARIFHGRAMFGKGIPAPEIKGKV
                     ISRREFSASSLEIILNEAETPAAGSGRMD"
     gene            6122..6988
                     /gene="prmC"
                     /locus_tag="PLZ75_01125"
     CDS             6122..6988
                     /gene="prmC"
                     /locus_tag="PLZ75_01125"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOS71031.1"
                     /translation="MAVNIQTIKDIKLYLKDELEDVYPVHEINAMSAIILREILETPG
                     IHHLALPETRLHHEQARKIMSISRELRKGKPLQYILGKTEFCNCTIYLNEHTLIPRPE
                     TEELTDLVIRENRQNRFPVLDVGTGSGCIAIALAVNLPRCKVSGFDISAEAVKKAREN
                     AALNNAPVTFTVADLFTVSRKQFHGTGIVVSNPPYVRESEKRFMAGNVLDYEPHSALF
                     VPDSDPLVFYRAIADLASEILVPDGKIYLEINEALGAELSDLLKSSGFPSPEIIRDIN
                     NRERIIKAARHD"
     gene            6981..7457
                     /locus_tag="PLZ75_01130"
     CDS             6981..7457
                     /locus_tag="PLZ75_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOS71032.1"
                     /translation="MTENDLFKTLLSRSMALCSKREYCTDDIKSRLKLWGLGEKDSER
                     IITILTKENFISDERYARAFVNDKFICNKWGKVKISAHLKAKRIPQEIISEALNSIDD
                     ETYVKTVRDIISARRGYIRAKNQYDLKGRLLRYGLSKGYESGLLYDILNDLKNDPE"
     gene            complement(7594..8022)
                     /locus_tag="PLZ75_01135"
     CDS             complement(7594..8022)
                     /locus_tag="PLZ75_01135"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOS71033.1"
                     /translation="MEAEPREKAVQTEPYLVINMALALVIALILLYSLVFSPAGDNYP
                     IPCIHEKISGEKCPSCGISHSFSLIARGRINEAYSWNSRGMSVFIFFAAQLILRISFS
                     RSYLKNPDSRRELVLTDITGSILMFLTAFMPFIVFLVRML"
     gene            complement(8024..8545)
                     /locus_tag="PLZ75_01140"
     CDS             complement(8024..8545)
                     /locus_tag="PLZ75_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOS71034.1"
                     /translation="MEDQNQNPEMPGNQERGSSGQSLGVASLITAIITFVLAVIPCVG
                     LMAVIPGIITIVLASVGLSRSSGNDSRRGLLVAGLVIGIVATLISISQIYVAGEIFRK
                     SGKWPTKIEKMVDEIQKEIDKAIEEASVDIRIENGDNNADISPASGKTARERTLEELE
                     TGKSSLSDTLSSP"
     gene            complement(8650..10710)
                     /gene="scpA"
                     /locus_tag="PLZ75_01145"
     CDS             complement(8650..10710)
                     /gene="scpA"
                     /locus_tag="PLZ75_01145"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOS71035.1"
                     /translation="MPANPEGSAVKNCYTSADLYGMEHLGYAAGLPPFLRGPYSAMYA
                     LQPWTIRQYAGYSTAEDSNAFYRRNLEAGQTGLSVAFDLPTHRGYDSDHERVAGDVGM
                     AGVAVDSVADMKILFDRIPLDRISVSMTMNGAVLPVMAFFIVTALEQGVRPEDLTGTI
                     QNDILKEFMVRNTYIYPPDFSMRIVSDIFRYTSEKMPRFNSISVSGYHMQEAGATSDI
                     ELAYTLANGLEYLRTGIRAGLDIDTLAPRISFFWGTGMDYFMEIAKMRAARMLWAKIV
                     KGFNPGNPKSMALRAHCQTSGWSLTEQDPYNNVGRTCIEAMAATAGHTQSLHTNALDE
                     ALALPTDFSAAIARDTQIYIREETEICRAVDPWAGSYYVELLTDRIARRAWEHITEIE
                     KLGGMTKAIESGIPRMRIEEAAARTQAEIDSGLKVIVGSNRYRPEGEKALEILEVDNT
                     AVRNSQIKRLAELKASRNEAECRSALDAITECVRTGKGNLLELAVRAASARATLGEIS
                     YACEKITGRYKAVIRSVSGVYSSGYGPDRDFTEAKALAAGFAEKEGRQPRIMIAKIGQ
                     DGHDRGAKVVATGYADIGFDVDIGPLFQTPEEAARQAVENDVHVLGVSSLAAGHKAHI
                     PKVLDELRELGREDILVIAGGVIPPQDYQFLFDAGVAAIFGPGTSVAKAAKQILEILL
                     EAFE"
     gene            complement(10921..12051)
                     /locus_tag="PLZ75_01150"
                     /pseudo
     CDS             complement(10921..12051)
                     /locus_tag="PLZ75_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
     gene            complement(12205..13294)
                     /gene="prfB"
                     /locus_tag="PLZ75_01155"
     CDS             complement(join(12205..13230,13232..13294))
                     /gene="prfB"
                     /locus_tag="PLZ75_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137305346.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOS71036.1"
                     /translation="MVSSEQIKSLSGRLEALRRYLDIDGKLKQIEEKESLTRRPGFWD
                     DPKQAGETGKSISRLKSWTEEFDRLKTAIDDLEVINDFFHEGEATEKDLDDQYEKCIE
                     LIENLEVRNMLGKKEDQLGAILKINSGAGGTESNDWAAMLMRMYLRWAERNNYKTRQL
                     DFLPGDEAGIKSVTIEVEGDQAYGYLKSENGVHRLVRISPFNAQGKRQTTFASVFISP
                     LVDENIKIEINPSDISWETYRSSGAGGQNVNKVETAVRLRHHPTGLVIENQETRSQLN
                     NKENAMRILKSHLYEMELRKRMEEQSRIEGEKKKIEWGSQIRSYVMHPYKMVKDLRTG
                     YETGNVQAVLDGDLNDFIRSYLMEFGGR"
     gene            13567..14166
                     /locus_tag="PLZ75_01160"
     CDS             13567..14166
                     /locus_tag="PLZ75_01160"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOS71037.1"
                     /translation="MLKSYMYRNLVEAGCDEAGRGCLAGPVTAAAVILPRNYKNPDLN
                     DSKKLTAKQRNILRNEIMDTALAWHVAFIDNMEIDSLNILRATFKAMHLAIGGLKKVP
                     QHLLVDGNRFQPYGNIEHTAVVAGDSLFLPIAAASVLAKTFRDEFMEKIHEEYPCYGW
                     NTNKGYGTPFHRKAILRHGISPYHRKSFSLFNTQMSFGF"
     gene            14267..16420
                     /locus_tag="PLZ75_01165"
     CDS             14267..16420
                     /locus_tag="PLZ75_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOS71038.1"
                     /translation="MKKSIQLLFLLLICSGSVSKADEGMWLLPMIEKLNIGTMTTLGL
                     KLSANDIYSINNASLKDAVVSFMDGGCTGELVSPEGLMLTNHHCGYSAIQSHSSLEHN
                     YLRDGFWAMTREEELPNPGLSVSFLIRIEDVSDQVLSELKPEMSESQREKAISAVSHS
                     IERTASRENGYRASVVSFYGGNYFYLLVYERYTDVRLVGAPPSSIGSFGDDTDNWEWP
                     RHTGDFSMFRIYTGPDGKPAPYSKDNIPLNSRNWLRVSIKDLNEGDFTMVMGYPGRTQ
                     RYYTSWEVGELLQITNPNRIKIRGIKQESWMEDMLADPAVNIQYASKYSTSSNYWKYS
                     IGQNRQLVSLAVTDKKKEIENRFNTWVNEDAGRKSEYGEALNLIRSSIEGRAVYQNAY
                     QYLNECMRGCEIFDFMMYTAALSEALRSGDREKTERAAERIKNIAPGLYKDYNPPTDQ
                     KTTKKMLQLYRNDVPAKFHPSFYSEIIDRKFRGNIGKYVDDMFRRSVFAGEKKLISFL
                     SSPKLKILENDPVAQAVNSIIEVRNLLTDELDKFDGKLAEGRRLWIAALREMIPGKTL
                     YPDANSTMRLSYGTIRDYEPRDAVTYKYFTTLDGVIDKYRPGDYEFDLPPRLLELHKG
                     KEYGRYASSEGYLPVNFLTTNDITGGNSGSPVMNANGELIGLAFDGNWEAMSGDVFYE
                     PEVQRTIAVDIRYVLWITDIYAGARHLVDEMTIVE"
     gene            16437..17021
                     /locus_tag="PLZ75_01170"
     CDS             16437..17021
                     /locus_tag="PLZ75_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOS71039.1"
                     /translation="MQLSFIEILGAFMVLFAIIDITGSIPVIIDIKSKGIEVHSLKVT
                     LVSGLILLLFLLVGTPLLGVFGIDVSAFAIAGAFIIFLIGMEMILGIEIFQQNSHGTG
                     AIFPIAFPLIAGAGSITTILSLKAEYHTINILIALVLNMVIVYLVLRLTSLFERLIGP
                     GGLQILKKVFGVILLSIAIKLFLTNTGIALPHGN"
     gene            17011..17505
                     /gene="rnhA"
                     /locus_tag="PLZ75_01175"
     CDS             17011..17505
                     /gene="rnhA"
                     /locus_tag="PLZ75_01175"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOS71040.1"
                     /translation="MATDVTIYTDGAARGNPGPGGYGVVLVSGRHRLEKTEGFRLTTN
                     NRMELLAVITGLEALKQPGSNVTVYTDSKYVADAVEKGWLFRWEAKSFRKTKNPDLWK
                     RFLDIYRKHNVRFIWIKGHSGHPENELCDRMAVESSMGSELSDDTGYRPDRDDVTIFS
                     EHVE"
     gene            17557..17973
                     /locus_tag="PLZ75_01180"
     CDS             17557..17973
                     /locus_tag="PLZ75_01180"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOS71041.1"
                     /translation="MQKLVIEPTHNTPGIILSPGERIFSIEGMSAPEDVRTIYYPVLE
                     WMKEFNREVQEGSQKTFTEEDPLILKIDLNYFNSASAKFLFDILLEFKELSDAGVPVV
                     TEWHYEEDDTDMMEAGEDIAEMIEIRLKFMPKPPDA"
     gene            17973..18467
                     /locus_tag="PLZ75_01185"
     CDS             17973..18467
                     /locus_tag="PLZ75_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOS71042.1"
                     /translation="MRGQKELYEILAGLDIGFEYHEHPPLNTIEDAIIHWKDFKSGRC
                     KNIFFRNHKGNRHYLVILEHLRQLDIRDLEKRLRQGKLTFASGKRLRKYLGVEPGSVS
                     PFGLVNDHENHVHLFIDEQLNEYERLAFHPNVNTASLVISRKDFLKFLDYTGNSYEFI
                     RLYD"
     gene            complement(18492..19292)
                     /locus_tag="PLZ75_01190"
     CDS             complement(18492..19292)
                     /locus_tag="PLZ75_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOS71043.1"
                     /translation="MLTRRSFISKSAVAASAAIVAPSIAAGSRRSASGSLLAGKKILF
                     VWGGWMGHEPDKCRDIFVPWMESEGARLTVSDTLDSYLDPALKDYDLIIQAWTMGTIE
                     NNQEKALLEAIRSGVGIAGWHGGLGDSFRNNTEYQFMVGGQWVAHPGGVIDYRVNITD
                     RNDPVTKGLSDFDMHSEQYYVHVDPNVKVLATTTFSDEHSPWIGGTVVPVVWKKVYGR
                     GRVFYSSLGHVAADFKVPGALEIQKRGIIWASGSKYEPMEEWKQPVYG"
     gene            19441..22089
                     /locus_tag="PLZ75_01195"
     CDS             19441..22089
                     /locus_tag="PLZ75_01195"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOS71044.1"
                     /translation="MLKLILPVLVILTAVRILPAQEQSHGKKLYKATRINEAPVINGI
                     LDEKLWEAGEWVDDFIQYEPYNGKAASQRTEFKILFDNDNLYAGIRAYDTSPDSIVRR
                     LTRRDQADGDMLALIIDSFHDLRTGFTFGISAAGVKQDMIYTSDGQNQDNSWNPNWWA
                     KTSMDNEGWTAEIKIPFNELRFDKSNGDVWGFQVARILYRKNETSLWQHIPKDSPGLI
                     HMLGELTGLVEIKPRRVFDITPYGVARAEKFKAESANPFLEKGRRFGLGGGADAKIGI
                     TSNMTMDLTINPDFGQVEADPSVVNLTAYETFYKERRPFFIEGSNITSYNIGLGEGDI
                     GNDNLFYSRRIGRQPQGRPELKDGWHADIPINTKILGAAKLTGKTGKGLSLGFVEAVT
                     AAEKAEIDTVGGRIRQTVEPLTSYFIGRVQKDFREGKTILGGIFTGTNRDIDDRLAEF
                     MHKSAYSGGIDFTQYTKGKNWMLNINTAFSHVSGTREAIQLTQKSPVRYFQRPDRKHN
                     RYDPERASLAGTGGRIGIQKLNGHLLLLGSTMWRSPGFEINDLGYIQTADQIMSVLVA
                     GYSEWEPRGIYRNYNFNADIWAVHNFAGQHLGTGIESYGSMGFKNYWNAWTGGNLSFS
                     QLSPYILRGGPMMKIPGTINLRAGFTSDNRKKLNFSFYGSLSKGLEKDSRNLYSELKI
                     DYRPVNYITLSFKPSFNSSFTELQYVTKLTKEEKDSYIFAGIDRKTISASFRVNVNLA
                     PDLTLQYWGQPFVASGKYYDHKVITDPVAANFRDRFLTFTREQIRFDPGQGKYIVDTD
                     LNGIPDFEFRNNNFIVREFLSNFVLRWEFNPGSALYLVWSQTRNYSDNSSDIRYFSDL
                     GDLFNRDNDIPHNIFLIKFSYRFALK"
     gene            22484..>23161
                     /locus_tag="PLZ75_01200"
     CDS             22484..>23161
                     /locus_tag="PLZ75_01200"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS71045.1"
                     /translation="MTSLLTGLLVSLPQLSLAGGEENPGSMDDNPAVELMQQRVTGTI
                     TDASTGDPLVGVNIVVEGTTTGTMTDFNGKYTIEVPGRNAVLSISYIGYIALKVNVEG
                     RTVIDVSMESDLRALEEVVVTGYGTVRKSDLTGSVSSIKTEQILQLPTQRVDQAIQGR
                     APGVFILNTDGSPGGNTMIRIRGSNSIHGGNDPLIIVDGLQGANINQLNPNDIASMEI
                     LKDAAATA"
BASE COUNT         6312 a         5595 c         5371 g         5883 t
ORIGIN      
        1 gccagcttct tcgctatttc tgtttctttc gttttttcaa ggccggattt ttttgcatct
       61 atctttgcct cattctgctt catccactct tcaaacctcc tgtcagcttc ggcctcgtca
      121 aatgcaccct ttctgacgcc ttcaagaagg tgtttccgga taagaacacc cttgtaggaa
      181 aggatagccc tgcatgtttc agtaggtaat gcgccttttt gcagccaacc aagagcctta
      241 tcgaaatcga gttcgatcgt ggcggggtta gtgatcgggt tgtagcttcc gatcttttca
      301 atatatctgc catcccgtgg cgacctgctg tctgctacca ctatgtggta gtgggccagt
      361 ttcttacgtc ctttcctggc caatctgatt ttaactgcca tttattacaa acgctttttt
      421 acctgtttaa aaaattgtgg gtgcaaatat aaattttttt attaacaatc atgtttattt
      481 acctgccttt attcattttt aaatattttt gtacgtcagc cggtcatccc taatttctgt
      541 tgttatggca atcttctctc atttacacgt acatacccag tattccatcc ttgacggagc
      601 ctcaagcatt cccctgcttg tcgacagggt gaaatccctg ggaatggaag cggttgcaat
      661 aacagatcat ggaaatatgt tcggggtgaa ggagtttcac aatgcagcca gcaggaaagg
      721 catcaagccc attatcggat gcgaggtcta tgtggccagg cggtccatat ctgaaacacc
      781 cggaaaggaa gaccgttcgg gagatcatct catcctgctg gcaaagaacc tgaagggtta
      841 caggaatctg ataaaactca tttccctttc atggataaag ggattttatt acaccccgcg
      901 gattgacaag gaactgctcg ccgaatacag tgagggcctg atagcatgtt cagcctgcat
      961 tgccggtgag atacccgaca aaatacttaa tgtcagtatt gaagctgcgg aagaagcact
     1021 gaaaagctat atcagcatat tcggaaagga tttctacctt gaagtacaga ggcatgagac
     1081 ccatgaacct gatgccgacc cgacggtttt tcccaaacag cagagggtga tcgaagcagt
     1141 caaaaaactt gctgcccggc ataatgtcgg aatcatcgca acaaatgatg tccatttcgt
     1201 aaatgccggg gatgctgaag cccacgaccg gcttatctgc ataaatacgg caaaggatat
     1261 tgatgatccg gacagactca ggtatacaaa gcaggaatat ctcaagagcg aggaggaaat
     1321 gaggaggatt ttcgccgaca tgcctgaggc tgttgacaat gtagcggaag ttgtcgggaa
     1381 aattgaaaaa tacaagctgg atcacgatcc catcatgcct gattttgacc tgccggccgg
     1441 atttgaggga aaggatgaat acctgaggca tcttacctat gagggtgcca gggagcgatg
     1501 gggcagcccg aacagcgaac agaccgaacg gatagatttc gaactgcaga ctattgccaa
     1561 aatgggattc cccggatatt tcctgatagt gtgggatttt ctcaaggcag cacgggaaat
     1621 gggtgtatcc gtgggtccgg gaagaggatc cgccgcagga tcggcagtgg cttactgtct
     1681 gaagattacg gatattgacc ccattgaata cggactcctt tttgagcgct tcctgaatct
     1741 cgaccgcaca tcaatgcctg atattgacat tgatttcgac gaggacggaa gggagtctgt
     1801 cctgaattat gtggtggaca aatacggaca tgacagggtg gcccatatca tcacattcgg
     1861 gtccatggcc gcaaaaatgg caatccggga tgtggcccgg gtgcagaaac tgcccctggc
     1921 cgatgcggac cgtcttgcaa agctcgtgcc cgaaaagccc ggcatcactc tgctccaggc
     1981 ctatcaggaa gttcccgagc ttgcaaagga aagggaatca tccaacacgc ttgtctcaca
     2041 gacactcaaa tatgctgaag ttctcgaagg ttcagtaagg caaaccggcg ttcatgcctg
     2101 cggcgtgata ataggcaggg aatcccttga caattatatt cccctgtcca ctgccaagga
     2161 taccaggctc tatgtaaccc agtacgacgg aaaccatgtc caggcggtgg gactgcttaa
     2221 aatggatttc ctgggactga agaccctttc aataataaag gatgccatac ggaatattaa
     2281 gaacagcagg ggcattgacc tggatattga ttcaataccg ctggatgatg cgaaaaccta
     2341 tgagctgttc agtaacggcg aaacaacagc aatctttcag ttcgaatcgg ccgggatgaa
     2401 aaaatacctg aaggaactca tgccgaacag attcggggac ctgatagcca tgaatgccct
     2461 ttacaggccc ggaccgatgg attatatccg tgaatacatc agaaggaaga acgcgggagg
     2521 gaaggttgaa tacaaacttc ccgtgatgga aaaatacctt agcgacacat atggcataac
     2581 tgtctatcag gaacaggtta tgcttctgtc acaggaactt gccggcttca caaagggtga
     2641 agccgacaac ctccggaagg caatgggcaa gaagaaacgt tccatcatgg acgaaatgaa
     2701 ggtcaagttc tttgaaggaa gcctgaaaaa gggccatgac gaacagacca tcagtgagat
     2761 atggaacgac tgggtttcat ttgccgaata tgcattcaac aaatcccatt ccacctgcta
     2821 tgcctatgtg gcatatcaga caggatacct gaaagcccat tatcccgcgg aattcatggc
     2881 tgcagttctc agccgcaata tcagcgatat caagaagatc accaccttca tggacgaaac
     2941 aaggcgtatg ggaatggaag tgctcggacc ggacgtgaat gaaagctatg tcaatttcac
     3001 ggtaaacaag gacggaaata tacggttcgg actcggggca ataaagggtg tcggcgagaa
     3061 tgccgtaatg cagcttatcg gggaaaggga aaaaaacggg ctgttcaggg atatatacga
     3121 tcttgtggaa cgcgttaatc ttaactcatt caacaagaaa aaccttgagg caatggctgt
     3181 tgccggtgcc tttgactgtt ttgaggatat gtccagggca cagtattttg cgctggacaa
     3241 cagggattcc agtttcatag aaagcctgat ccgttacggc caaagtgcaa aagccataag
     3301 gcagaacaac agtcagacgc tgttcgggga atcgggcggc tttgatcttg tcagaccgct
     3361 tcctgccgcc tgcagtgact ggcccaaact ggaaaagctc aacagggaaa aggaggtgat
     3421 aggaatatac ctctcctcac atcccctcga cgatttcaga ctggaaataa atactttcac
     3481 aacggctaca cttgccgacc tgcagaatct ccggaattat ctcgaccgcg aagtgaccgt
     3541 ggcgggaatg gtcacagaca ccagaaacgg tatcgggaag aataacaagc cctatggctc
     3601 acttaccctg caggattata ccgattcatt cagattcatg ctttttgaca gggactatat
     3661 tgacaacagc aagtttttta ctattgggta ctacctgctg gtcaggggca aagtccgtaa
     3721 gagaaaatac cgggaagacg aactcgaatt cagcataagt cagataagcc ttctgtcaag
     3781 tgtgaaggat gaactcatta aaagcataac cctgaagatt gatcccgtaa acatcgacgg
     3841 ggagatgatc cgtgaactga gacagcttgt aagtgataac aagggagaaa cggaaatgag
     3901 attcatgttt ctagacactg aagacaggat atcacttcca atgttctcaa ggacactgag
     3961 ggtaaggctg aacaatgatt ttatttcata tctcgaagat cacccttcta tagattttaa
     4021 agttaattag ctatatttgg ccactgtttt ttttaatcct tgaactatgg caattgaagt
     4081 aagcgatgga aattttgaag aagtggttat tcaatcggaa ataccggtta ttgttgattt
     4141 ctgggctgag tggtgcggac catgcagaat gatctccccc atcctggaag aaatatcaaa
     4201 agagtacacg ggaaaggctt tggttacaaa gtgcgatgtt gacaacagcc ccagaacaac
     4261 agcaaaatac ggcataagga atattcccac agtattgttt ttcaaaaagg gcgaagttgc
     4321 cgacaagcag gtgggtgccg tgcccaaaag caatttcgtc aataaattga acgccattct
     4381 ctgattcttt atatcatcat actgcagaac cattcgtgaa tgatgaccgt gtgtgtcttt
     4441 gctgcctcca gcagcaaatt tgaaaatgaa tacggcaagg atgcctcccg cctgggatcg
     4501 cttcttgcca aagccggcat cagtgttgtt tacggtggag gcggaatagg gctgatgaat
     4561 gcccttgcca gtgcagtcat cagcaacaat ggtaatatta ccggggtgat ccctgccttc
     4621 atgcaggcgg aagggtgggg aaatgacggc gagaaccgga caatcgtgac atccgacatg
     4681 aatgaacgga aaagaaaaat gttcggcctt tccgatgcca tcgtcgcatt gcccggaggc
     4741 atgggtacac tggaagaact gaccgaagcc attaccctca agcagctggg actcttcaac
     4801 ggaccggtaa taatcctgaa tacctgcgga ttctatgact acttcctgaa attcaccgat
     4861 aacatggtcg agaataattt tctgagaaag gaacacaagg ggatgtggga agtggtaaac
     4921 agtcccgagg aagtcatgga agccctgagc cggaaagcgg gaaaaaacag gacatggaga
     4981 agcatagcca ggatatgatt cattaatcca tcctgccgga tcctgctgcg ggtgtttcag
     5041 cttcgttcaa aataatctca agactgctgg cactgaattc cctccgggaa ataaccttcc
     5101 cctttatttc cggagccggt attcctttcc cgaacattgc cctgccgtga aaaatccttg
     5161 cttcatccca caggcctccg gatatgaaca tacccagtac ctctgccccg ccttcaatga
     5221 aaagcgactg aatccccgaa tcatgcagat ggctcagtat ctgaacagct gatgaaagac
     5281 cgcgctcaag cgggactatc acggcaggat atccccctgc ggcttccgga tcatgagtaa
     5341 acaccgtcac actgccgccg gtatgaaaaa taccggaatc cttgtcaagg cggcccgagc
     5401 tgctgagaat cagcttaagg ggatcattgc ccgcccaatc ccgcacgttg agaagggaat
     5461 catcagccct aagggttccg gcaccgacaa gaaccgactg ttccgcggca cgccacctgt
     5521 gtacaagaac cctttcgggc ttccctgtga tccacgtcgg ccttctacgg ccgttgtaaa
     5581 tatctctttc aacatccagg aaagagtcag cactttccgc ccactttaaa atcacatagg
     5641 gccgtttttt ctcgtgaaag gtgaaaaatc ttctgttcag ccagcggcat tcttcctcaa
     5701 gaactcccgt aatcacctca catcccgcgt ttctcagggc tgccacaccc cggcctccga
     5761 ccctgtcgct ggtatcaatc gttccggcaa caattctttt tattcccgtt tcaataatca
     5821 ggtccgtaca gggcggggtt ttaccatgat gcacacaggg ctcaaggttt gtataaagta
     5881 cggaatcctc cggcaatcca cggctgatca cggaattaac agccactctc tctgcatgtg
     5941 cttctcccgc ccttacatga aatccctccc ctgttatcct gccctgggag acgataacgg
     6001 aaccgaccag gggattggga taggtattgc cttctgcccc tgcagccaga tcaatacatc
     6061 tccgcatgaa ttttatatct tcggatgccg gcatttccct tcaaaaggct aattttgact
     6121 tatggctgtc aatatacaaa caataaagga tataaaactc tacctcaagg atgaacttga
     6181 agatgtatat cctgtccatg agatcaatgc aatgtcagcc attatcctca gggaaatcct
     6241 ggaaacaccg ggaattcatc atcttgccct gcccgaaacc aggcttcatc atgaacaggc
     6301 ccggaaaata atgtcaatca gccgtgagct cagaaaggga aagcctcttc agtatatcct
     6361 gggaaaaacc gaattctgca actgtaccat atatcttaat gagcacactc tcattccgcg
     6421 tccggaaacg gaagaactga ccgatctcgt aataagggag aacaggcaga accgctttcc
     6481 tgtcctggat gtgggaacag gatcgggctg tattgccatt gccctggcag tcaatcttcc
     6541 tcgctgcaaa gtgagcggat ttgatatttc ggcggaagct gtgaaaaaag cacgggaaaa
     6601 tgctgctctg aacaatgccc cggttacatt taccgtagcc gaccttttca cagtaagccg
     6661 gaaacagttt catggaaccg gcattgtggt aagcaatccg ccctatgtac gtgaatcgga
     6721 aaagaggttc atggcaggga atgtacttga ctatgaaccg cattcagcac tttttgtgcc
     6781 ggattccgat cccctggtct tttacagggc cattgctgac ctggcatcgg agatccttgt
     6841 tccggacggg aaaatatatc tggaaataaa cgaagccctc ggtgcagaat tgtcggatct
     6901 gctgaaatca tcgggctttc cttctccgga aataatcagg gatatcaaca acagggaaag
     6961 aataataaaa gctgccaggc atgactgaga acgatctttt taaaactctg ttgtccagat
     7021 caatggcact ctgttcaaag agagaatact gtacggatga tattaaatcc agactgaagt
     7081 tatgggggct cggggaaaag gacagtgaaa ggataatcac aatcctgaca aaagagaatt
     7141 tcatcagtga tgaacgctac gccagagcat ttgtcaatga taaattcatc tgcaacaaat
     7201 gggggaaagt caaaatttcc gcccacctga aggcaaaacg cattcctcag gaaattattt
     7261 ccgaagccct gaattcaatt gatgatgaaa cttatgtaaa aacagtaagg gatatcatct
     7321 ctgcccgccg gggttacatc agggcaaaaa accagtatga cctgaaaggc agactgctga
     7381 gatacggcct ttcaaaagga tatgaaagcg gcctgctgta cgatatactg aatgacctta
     7441 agaatgaccc tgaataataa taaagggtct tcagaataaa cctgagggat agtaaatgat
     7501 tttcaatggc ctctgaatga taggaagtga ttctcaatga tccgccagtt tatgactgta
     7561 atccctggaa tacaaaacgg atattttccg gcatcacagc atcctgacaa ggaaaacaat
     7621 gaagggcatg aatgccgtca ggaacatcag gatgcttcct gttatgtcag tcaggaccag
     7681 ttccctgcgt gaatccggat ttttcaggta agatcttgag aaagagattc tcagaatcag
     7741 ctgtgctgcg aaaaaaataa acacactcat tcccctgctg ttccatgagt aagcctcgtt
     7801 tatcctgccc ctggcaatga gggagaaact gtgtgaaata ccgcaggagg gacatttctc
     7861 cccgcttatc ttttcatgta tacagggtat ggggtaatta tcccctgcgg gagaaaaaac
     7921 cagggaatac agcagaatga gtgcgataac cagggccaat gccatgttta tcaccaggta
     7981 aggctctgtc tgaactgctt tttcccttgg ttcagcttcc atgctacgga cttgagagag
     8041 tgtcactgag ggaagatttt ccggtttcaa gttcctcaag ggtcctttcc cttgctgttt
     8101 taccggaagc cggactgata tcagcgttat tgtctccgtt ttcaatcctg atgtccacac
     8161 tcgcttcttc aatggccttg tctatttcct tctgaatctc atcaaccatc ttttcaatct
     8221 tagtcggcca cttgcccgat ttcctgaaaa tctcacccgc aacataaatc tgggaaattg
     8281 aaatcagcgt ggccacaatt cctatcacca gacccgcaac aagaaggccg cggcgggaat
     8341 cattccccga tgaccgggaa aggcctaccg aggcaagcac aatggtgatt attcccggaa
     8401 ttacagccat tagccccaca cagggtatta ctgcaagaac aaacgttatg attgcagtga
     8461 taagggaagc taccccaagg ctctgtcccg atgatcctct ttcctgattc ccgggcattt
     8521 ccggattctg attttgatct tccatgttta gtaaggtatt tctgttttca ttcctttgct
     8581 gtgatatcgt taatatcctg cattaaagac gtgagaagac cgggaatgat gcttcgggtc
     8641 cggttttttt tattcaaatg cctccagcag tatttcaagg atttgttttg ccgcctttgc
     8701 cactgatgtc cccggaccaa aaatggctgc aacgcctgca tcaaaaagga actgatagtc
     8761 ctgaggagga ataactcctc ctgcaatcac caggatatct tccctcccca gttccctgag
     8821 ttcatccaga actttgggaa tatgtgcctt gtgtcctgct gcaagactgg aaacgccaag
     8881 aacatggaca tcattctcca ctgcctgcct tgccgcttcc tccggagtct ggaacaaggg
     8941 tccgatatcc acatcgaatc ctatatcggc atagcccgtg gcaaccactt tcgcaccccg
     9001 gtcatggccg tcctgaccta tctttgcaat catgatccgg ggctggcgcc cctctttttc
     9061 agcaaaaccg gcagccaggg cttttgcctc ggtaaaatcc cggtccggcc cgtagcctga
     9121 tgaatacaca cctgaaactg atcgtatcac tgctttatat cttcctgtaa ttttttcaca
     9181 agcataggat atctctccga gggttgccct ggcggaagca gcccttacgg ccagttccag
     9241 caggtttccc ttgccggtcc tcacacattc cgtgattgca tcaagggccg aacggcattc
     9301 agcctcattc cttgatgcct tcagctcagc aagtcttttt atctgggaat tcctgacagc
     9361 tgtattatcc acttccagga tttcaagtgc cttctcccct tccggccggt acctgttact
     9421 tcccacaatc actttcagtc ccgaatcaat ttccgcctga gtccttgcag ccgcttcctc
     9481 tatccgcatg cggggaattc ccgactcaat ggcttttgtc attcccccga gcttttcaat
     9541 ctccgtgatg tgctcccagg ccctgcgggc aatccggtct gtaagtaatt caacataata
     9601 ggagccagcc catggatcaa cggccctgca tatttccgtt tcctcccgta tgtatatctg
     9661 ggtatccctt gcaatggcgg cggagaaatc ggtgggcagt gccagtgcct catcaagggc
     9721 atttgtgtga aggctctgtg tatgtccggc tgttgcagcc atggcctcaa tgcatgtcct
     9781 gccgacattg ttgtagggat cctgttccgt gaggctccat cccgaagtct ggcaatgggc
     9841 tctcagagcc attgatttcg gatttccggg attgaagccc ttcacgattt ttgcccacag
     9901 catcctggct gcacgcatct tggctatttc catgaaataa tccattccgg tgccccagaa
     9961 aaaagatatc cgaggggcaa gggtgtcgat atccagtcct gcccttattc ctgtacgcag
    10021 gtattccaga ccgttggcga gagtataggc aagttctatg tcggatgttg caccggcttc
    10081 ctgcatatgg taacccgaaa cgctgatcga attgaacctg ggcatttttt cactcgtata
    10141 cctgaaaata tcagatacta tcctcatgga aaaatcaggc gggtatatat aagtgttcct
    10201 taccatgaat tccttgagta tgtcattctg gatggtcccg gtgagatcct ccggccttac
    10261 tccctgctca agcgcggtga cgatgaagaa tgccatcacc ggaagcactg ccccgttcat
    10321 ggtcatcgac accgatatac ggtcgagcgg tatgcggtca aacagaattt tcatgtcggc
    10381 taccgaatca actgcaacac ccgccatgcc gacatccccg gcaacccttt catgatccga
    10441 gtcataaccg cggtgtgtag gaaggtcgaa agccaccgac aggccggtct gtccggcttc
    10501 aaggttcctg cggtaaaagg catttgaatc ctcagcggtg gaatatcctg catattgcct
    10561 gatggtccag ggctgcagtg catacattgc tgaataggga cccctgagga agggaggcag
    10621 ccccgcagca tagcccagat gctccattcc gtaaaggtcg gcggacgtat agcagttctt
    10681 gacggcactc ccttcaggat ttgcaggcat atcacccggt tttgtgacag gggatggcgg
    10741 attctttcca tcccggaaaa gacctgcagg accgtgtatg cggccggata cagattccgg
    10801 ttcatgtccg ggtccggcag gttcaggatc agcttcctgt tcctgccatg tttcaggaac
    10861 cggggccgga aacactatta ttttatccct tccctgtcct gtaaagcctg cggcccgttc
    10921 tgctatcctt tttccgacaa agccttttcc gagcgccctg atgaaaccgc cttccctttc
    10981 cgtttcaagg aaaagcttcc aggcactgtc tgctatcagg taagtgagtt tttcaatata
    11041 atatgacccg ccgccgggat cagccacctc cgcaaaccgg gcttcctccc tgagtatcag
    11101 ctgctgattc cttgctattc tttcgctgaa ctcatccggg tttccggaaa taatatcgaa
    11161 gggctcaaca gtcagcgagt cagcacctcc caggatggcc gacattgcct cagtctgggt
    11221 tctcagcata ttgatatggg aatctgccgt acttttattg atccgcattg tagtgcagtg
    11281 gatctccatt gcttcctgga tgccggttcc gaatttgttc aggataaccg accacaacag
    11341 gcgggcggct ctcagcctgg caatcccggg atagtactcc ggtccggcag cgaaggaaaa
    11401 ccggatcttc cgggctgctg tttcagggtc aatccccctt tcagccagtc cgtacaggta
    11461 ctcggcaccc attgacaatg caaaggcaag ttctgtcacc atatctgccc ccgcattcct
    11521 gatatttgcc gcattcaggt gaatggcacg gaagagtggc agttccgtgg tcagtccggc
    11581 cactcctgca aggtagtcga aacctttcgc gacaggaatg cagagtgttc cgttaagcat
    11641 caggcggcca agcggatctg tttcaactgc tcctcttatc ttttccggaa gaaatctctc
    11701 cgtattctgt atgaatgccg taaccagctc ttttgccctg ccgtcgctga gaaaattcag
    11761 ctccgtcgtt tcgggactga tacctgaaag gagaatatcc aggttttccg ggtttatgga
    11821 ttccggatca gcgagataga agccgagcga atcgatgccg tccctgacaa gcctgagagc
    11881 ttttctgttg gcggatgcat aatcggtcac cttcaggtcc tggcgtattc tccagctgtt
    11941 gtcacaggat tttttccccc gtatataggg gaattcaccg gctgcagcat gaagatattc
    12001 caggttttca atatcttcct gcctgtaaaa gggcatcaca tcaaatccca tggaagtttt
    12061 ccacaccagt ctgctgctga aatctgatcc tttcagatcg gatcttattc tgttcatcca
    12121 ttcttcagtg ctgacgggag gaaattcctc aaagagtttt tttctttctg acatctgcat
    12181 ttgcttattc ctggtccggg tagcttacct tcccccgaat tccatcaggt atgacctgat
    12241 gaaatcgttc agatcgccgt caagtacagc ctgcacatta ccggtttcat atccagtacg
    12301 gaggtccttc accatcttgt aggggtgcat tacatacgaa cggatctgtg agccccattc
    12361 gatttttttc ttttcccctt ctatccttga ctgttcctcc attctttttc tgagttccat
    12421 ctcatagaga tgggacttga ggatgcgcat ggcattctcc ttgttgttaa gctgggatct
    12481 tgtctcctgg ttttctatca ccagtccggt aggatgatgc ctcagcctga ctgcggtttc
    12541 gaccttgttg acattttgtc cgccggcacc gctggaacgg tatgtctccc agcttatatc
    12601 cgagggattg atttcaatct taatattttc gtccaccagg ggtgaaatga ataccgatgc
    12661 aaatgttgtc tgccgcttgc cctgtgcatt gaagggtgag atcctgacca ggcggtgaac
    12721 accgttttca cttttcagat agccgtatgc ctgatctcct tctacttcta ttgtaacaga
    12781 ttttattccg gcctcgtcgc cgggaaggaa gtcgagctga cgtgttttgt aattgttcct
    12841 ctcggcccag cgcagataca tgcgcatgag catggctgcc cagtcattgc tttcagtgcc
    12901 tccggcaccg gaatttattt tcagtatagc tccaagctga tcctcctttt tcccgagcat
    12961 attcctcacc tcaagattct cgatcagctc aatgcatttt tcatactggt cgtccagatc
    13021 cttttcggtg gcttctcctt catgaaagaa atcatttatc acttccagat catcaatggc
    13081 ggtttttagc ctgtcaaatt cctccgtcca gcttttcagc cgcgatatgc tctttccggt
    13141 ttcgcctgcc tgtttcggat catcccagaa tcccgggcgg cgggtcagac tttccttttc
    13201 ctcaatctgc ttcagctttc cgtcgatgtc aaagatacct ccttaacgcc tcaaggcgtc
    13261 cggaaagact ttttatctgt tcactgctta ccataatgga aattttcgca aaggtactta
    13321 aaaaacaaaa aaatcccgct ccaccgttac ggccaggtac aggattttta gtttttaaat
    13381 cttttgatta ttgactttcg ggttcaaata taatttaatt tttgacaaac aagcaaattt
    13441 atttgataaa aatgcatttt ttttacatta aattttttgt ttattctttt ttattacttt
    13501 ccctttaaat tatttttttg taaatatgcc gtgctttaaa tgttatgccc taattattaa
    13561 tagtttatgc tgaaatccta catgtaccgg aatttagttg aagccggatg tgacgaagcc
    13621 gggagaggat gccttgcagg accggttacg gctgcagcag taattttacc ccggaattat
    13681 aaaaatcctg acctgaatga ttcaaaaaaa ctgacagcaa aacaaagaaa tattctgagg
    13741 aatgaaataa tggatacagc cctggcatgg catgtggctt tcattgataa catggaaatt
    13801 gattccctga acatactgag agccactttc aaggcaatgc atctggccat cggcggcctt
    13861 aaaaaggttc cccagcatct gcttgttgac ggcaacaggt ttcagccata cggaaatatt
    13921 gaacatacag ccgtggttgc gggtgattcc ctttttcttc ctatagcggc agcttcggtg
    13981 cttgccaaaa cattccggga cgaattcatg gaaaagatcc atgaggaata tccctgttac
    14041 ggctggaaca caaacaaggg ctacggaact cccttccaca gaaaagcaat cctcagacac
    14101 ggaataagtc cctaccacag aaaatcgttt tcccttttca acacccagat gtcattcggt
    14161 ttctgagtaa gcagcccggt acggactgat cagttttcag ggctttcgtg atagacagaa
    14221 agaattatat ttgtattctg attaaaatta agcaattcca ctctttatga aaaaatcaat
    14281 tcaattgtta tttcttcttc tgatatgttc cggttcagtg tcaaaggctg atgaaggcat
    14341 gtggctttta cccatgatag agaaactgaa tatcggtacc atgaccacac tcggactgaa
    14401 actgtctgca aacgatatat acagcatcaa caatgcaagc ctgaaagatg cagtggtaag
    14461 cttcatggac ggaggctgca ccggtgaact ggtttcccca gagggactca tgctcaccaa
    14521 tcatcactgc ggctacagcg ccatccagtc acacagcagc cttgaacaca actatctcag
    14581 ggatggcttc tgggcaatga caagggaaga ggaactcccc aatcccgggc tttcggtatc
    14641 ctttctcata agaatagagg acgtaagcga tcaggtactg tcggagctga agccggaaat
    14701 gagtgaatcc cagagggaaa aagccataag tgccgtcagt cattcaattg aaaggactgc
    14761 ctcccgcgaa aatggttaca gggcttccgt tgtgtcattc tacggcggaa actatttcta
    14821 ccttctcgtt tacgaaagat ataccgacgt aaggctcgtc ggggctcctc cctcatccat
    14881 aggcagtttc ggggatgata ccgataactg ggaatggcct cggcataccg gagatttcag
    14941 catgttccgc atctacaccg gtcccgacgg aaaacccgca ccctattcaa aggataatat
    15001 cccgcttaat tccaggaact ggctaagagt atccataaag gatctgaacg agggagattt
    15061 taccatggtg atgggctatc ccggccggac acagcgatat tacacctcct gggaagtcgg
    15121 tgaattatta cagataacca atccaaaccg gataaagata aggggaataa agcaggaatc
    15181 atggatggaa gacatgctgg ccgaccccgc ggtaaacatc cagtatgcat ccaaatattc
    15241 aacatcctca aattactgga aatattccat cggacagaac aggcagctgg tctcccttgc
    15301 cgtgactgac aagaagaaag agattgaaaa caggttcaac acatgggtga atgaggatgc
    15361 cggaagaaaa tcggaatacg gggaggcact gaacctgatc cggtcgtcaa tagaagggcg
    15421 tgctgtatat cagaacgcat accagtacct gaatgaatgt atgaggggct gcgaaatatt
    15481 cgatttcatg atgtacactg cagcactgtc ggaagccctc aggtcgggcg acagggaaaa
    15541 gacagaaagg gcggcggaaa ggataaaaaa tatcgccccc ggtctgtaca aggattacaa
    15601 tccgcccacg gatcagaaaa ccacaaagaa aatgctccag ctttacagaa atgatgttcc
    15661 cgcaaaattc catccctcct tttacagtga aatcattgac aggaaattca ggggaaatat
    15721 cggaaaatat gttgacgata tgttcagacg gtctgtcttt gcaggcgaaa aaaaactgat
    15781 ctccttcctc tcatcaccaa aattgaagat ccttgagaac gatcctgttg cacaggctgt
    15841 taattctatc atcgaagtcc gcaacctcct tacggatgaa cttgacaaat ttgacggcaa
    15901 actggcggag ggccgcaggc tctggatagc tgcactgaga gaaatgattc ccggcaaaac
    15961 cctctaccct gatgccaatt ccacaatgcg gctttcatat ggtactatcc gcgactatga
    16021 gccgagggat gcagtcacgt acaaatactt cacgacactg gatggtgtga ttgacaaata
    16081 caggcccggc gactatgaat tcgatctgcc gcccagactg ctggaacttc ataaaggaaa
    16141 ggaatacggc agatatgctt cctctgaggg atatctcccg gtgaatttcc tgacaacaaa
    16201 tgatattaca ggaggtaatt caggaagtcc cgtaatgaat gcaaacgggg aactgatagg
    16261 cctggctttc gacggcaact gggaagcaat gagcggcgat gttttttatg aacccgaagt
    16321 tcagaggact attgctgtgg atatcaggta tgtactctgg ataacagata tatatgccgg
    16381 tgcccggcat cttgtggacg aaatgacaat agttgaataa acggaaggtg atataaatgc
    16441 agttgagttt tatcgagata cttggagcat tcatggtatt gtttgccata attgatatca
    16501 ccgggtcaat accggtaata atagacatca agtcaaaggg aatagaagtc cattcactga
    16561 aggtaaccct ggtttcaggg cttatactgc tccttttcct ccttgtgggc acgccccttc
    16621 tgggtgtttt cggaattgac gtgtcagcct ttgccatagc cggtgcattc atcatattcc
    16681 tgatcggaat ggaaatgata ctgggaatcg agatcttcca gcagaattcc cacggcacgg
    16741 gtgcaatctt tcccattgca tttcccctga ttgccggtgc cggatccatc accaccattc
    16801 tctccctgaa ggccgaatat catacaataa atattctgat agcacttgtg ctcaatatgg
    16861 ttatagtcta tctcgtcctc cggctgacaa gccttttcga aagactgatt ggtccgggcg
    16921 gactgcagat actgaaaaaa gtattcggag tcatacttct ttcaatagcc ataaagctct
    16981 tccttacaaa caccggtata gctctgcccc atggcaactg atgttacaat ctacactgac
    17041 ggtgcggcaa ggggaaatcc cggtcctggt ggatacggag tagtacttgt ttccggcagg
    17101 cacagacttg aaaaaactga aggattcagg ctcacaacaa ataaccggat ggaactgctc
    17161 gccgtgatta ccgggcttga ggcccttaag cagcccggaa gcaatgttac cgtgtatacc
    17221 gattcaaaat acgtggccga cgccgtggaa aagggatggc ttttccgctg ggaggcaaaa
    17281 tcattcagaa aaaccaaaaa tcccgatctc tggaaaagat ttctggacat ctaccgtaag
    17341 cataatgtca gattcatatg gataaaaggc cattcaggcc atcccgaaaa tgaactgtgc
    17401 gacaggatgg cagtggaatc ttcaatggga agtgaactga gcgacgatac cggctacaga
    17461 ccggaccgtg atgatgttac tattttcagt gaacatgttg aataataatc tttgcatata
    17521 agaaatatta atgcaaattt gtggaaactt ccgttaatgc aaaagctcgt catagaaccc
    17581 acacacaaca ctccgggtat aatactgtcg cccggggaaa ggattttttc aattgaggga
    17641 atgtctgcgc ctgaagatgt gcgcactatc tattatcccg ttcttgaatg gatgaaggaa
    17701 ttcaaccggg aagtgcagga aggcagtcaa aagacattca ccgaggagga tcctctgatt
    17761 ctcaaaattg acctgaacta tttcaattcg gcatcagcca agttcctttt cgatatcctg
    17821 cttgaattca aagaattatc agatgccggt gttcctgttg ttaccgaatg gcattacgag
    17881 gaagatgaca cggacatgat ggaagccggt gaggatattg cagaaatgat agaaataagg
    17941 ctgaagttca tgccaaaacc tcctgatgcc tgatgagggg ccagaaagaa ctttatgaaa
    18001 ttcttgccgg acttgatatc ggatttgaat atcatgaaca tcctccactt aataccatcg
    18061 aagatgcaat aattcactgg aaagatttca aatccggaag atgcaagaat atttttttca
    18121 gaaaccacaa gggcaacagg cactacctgg taatccttga gcatctcagg cagcttgaca
    18181 tcagggatct tgaaaaaaga ctccggcagg gaaagctgac atttgcctcc ggcaagcggc
    18241 tcaggaagta tctgggagtt gaacccggct cggtatcacc ctttggccta gtcaatgacc
    18301 acgaaaatca tgtccacctc ttcattgacg aacagctgaa tgaatatgaa agactggcat
    18361 tccaccccaa tgtgaacaca gcctccctgg tgatttccag aaaggacttc ctgaaattcc
    18421 ttgattatac gggaaacagc tatgagttca tcaggctgta cgactgacaa ggggcatgaa
    18481 cagctctctg cctatccgta aacaggctgc ttccactctt ccatcggttc gtacttgctt
    18541 ccggaggccc atattattcc tcttttctgt atctcaaggg ccccgggaac cttaaaatca
    18601 gctgcaacat gaccaaggga tgaatagaaa accctgcccc tgccgtaaac cttcttccac
    18661 accacgggaa caaccgttcc ccctatccag ggagaatgct catcactgaa tgttgttgtt
    18721 gccagaactt ttacattggg atcaacatgt acgtaatact gctccgaatg catgtcaaaa
    18781 tccgataaac cctttgtcac gggatcattc ctgtcggtaa tattcaccct gtagtctatc
    18841 acaccgcccg gatgggcaac ccactgacca ccaaccatga actggtattc ggtgttgttc
    18901 cggaatgaat cccccagccc gccgtgccag ccggctatcc cgaccccgct ccggatggct
    18961 tccagaagag ccttttcctg attgttttca atcgtaccca ttgtccatgc ctggattatc
    19021 agatcataat ccttaagagc aggatccagg tatgagtcca gcgtatccga cacagtcagc
    19081 ctggctccct ccgattccat ccagggaaca aaaatatcac ggcacttgtc aggttcatgt
    19141 cccatccagc ctccccagac aaacagtatt ttttttcctg caagcagcga tcctgaggcg
    19201 gatcttctgc tgcctgccgc tattgaaggt gcaacgattg cagctgatgc agccactgca
    19261 ctttttgaaa tgaacgatct gcgggttaac atctttattc ggtttccggc taagataagg
    19321 aatttgccgg tcggattcct gaaaaaaacc tgaaatttta taagaataaa ttatgttttc
    19381 tgcatcaaac cttttattta tccgtcatta taacaataca gcccataact gatgaatcac
    19441 atgctaaaat taattctgcc tgttcttgta atcctgactg cagtcaggat ccttccggca
    19501 caggagcagt ctcacgggaa aaaactatat aaggcaacaa gaataaatga ggcaccggta
    19561 ataaacggaa tcctggatga aaaactctgg gaagcaggtg agtgggttga cgattttata
    19621 cagtatgagc cctataacgg caaagccgcc tctcagagaa cggaattcaa aatactcttt
    19681 gacaatgata acctttatgc cggcatcagg gcctatgata ccagtcctga cagtatagta
    19741 agacgtctta cgcgaaggga tcaggcagac ggtgacatgc tggcgctgat catcgacagt
    19801 ttccatgatc tccgtaccgg cttcacattc ggcatcagcg ccgcgggagt gaaacaggat
    19861 atgatttata ccagtgacgg acagaatcag gataattcat ggaatcccaa ctggtgggca
    19921 aaaacatcga tggacaatga aggatggact gccgaaataa aaatcccctt caatgaactg
    19981 agatttgata aaagtaacgg tgatgtgtgg ggatttcagg tggcaagaat actttacagg
    20041 aaaaacgaaa catccctctg gcagcatata ccgaaagatt ctcccggact gatacatatg
    20101 cttggagaac tgacgggcct ggttgaaata aaaccgcgca gggtattcga tattactccg
    20161 tacggggtgg ccagagccga aaagttcaaa gccgaatcag caaacccctt tcttgaaaaa
    20221 gggaggagat tcggtttagg cggaggagct gatgccaaaa taggaatcac cagcaatatg
    20281 acaatggatc tgaccatcaa tcccgatttc ggacaggttg aagctgaccc gtcggtggtt
    20341 aatcttaccg cctatgaaac attctacaag gaaagaaggc cgtttttcat agagggaagc
    20401 aatataacaa gctataacat cggacttggt gaaggcgata taggaaatga caatctgttc
    20461 tattccaggc gtataggccg tcagccccag ggaagacctg agctgaagga tggatggcat
    20521 gccgatattc cgataaacac aaaaatcctt ggtgcggcca aactgacggg aaaaaccggc
    20581 aaaggcctgt cactgggttt cgttgaagct gtaacagccg ctgagaaagc agaaattgac
    20641 accgttggcg gcaggatccg gcagacagtc gaacctctta ccagctattt catcggaagg
    20701 gtacagaagg atttccgtga aggaaaaacc attctcgggg gaatattcac cggtaccaac
    20761 cgcgacattg atgaccgcct ggctgaattc atgcacaaat cggcatattc cggcggaata
    20821 gatttcactc agtatacaaa gggcaagaac tggatgctga acatcaatac ggcattcagc
    20881 cacgtcagcg gaacaaggga agccattcag cttacccaga aatcacctgt ccgctatttc
    20941 cagcggcccg acaggaaaca taacagatat gatcccgaaa gggcctcact cgcgggaacg
    21001 gggggaagga tagggataca gaagctcaac ggacatctcc tcctcctggg cagtaccatg
    21061 tggagaagtc cgggctttga aataaatgac ctgggatata ttcagacagc agaccagatt
    21121 atgtcggtgc tggttgccgg atattcggaa tgggagcccc gcggaatata caggaattac
    21181 aatttcaatg ccgatatctg ggccgttcat aatttcgcag gacagcacct tggtaccgga
    21241 attgaaagct acggatccat gggtttcaaa aattactgga acgcctggac cggtggtaac
    21301 ctgagcttca gtcagttgtc cccatacata ctcaggggcg gaccgatgat gaaaataccg
    21361 ggaaccatta acctgagagc cggattcaca agtgacaaca gaaagaaact gaatttctcg
    21421 ttttatggaa gtctgagcaa ggggctggaa aaagactccc ggaatctcta ttccgaactg
    21481 aaaatagatt acagacctgt caactacatc acactcagct tcaaaccctc attcaactcc
    21541 tcatttaccg agctgcaata tgttacaaag ctcacaaagg aagaaaaaga cagctatatc
    21601 tttgccggca ttgacaggaa aacaatcagt gcatcattca gggtcaacgt gaatcttgct
    21661 cccgatctca ctctgcagta ctggggacag ccctttgttg caagcgggaa atactatgat
    21721 cacaaggtca ttaccgaccc tgtggccgca aatttccgcg acaggttcct tacattcaca
    21781 cgcgaacaga tacgtttcga tcccgggcag ggtaaatata tcgttgatac tgacctgaat
    21841 ggcatacccg attttgaatt caggaacaac aattttattg tcagggaatt cctttcaaac
    21901 tttgtactga gatgggaatt caatcccggg tctgctctct acctcgtatg gagccagacc
    21961 aggaattatt cggacaacag cagcgatata cgctatttca gcgatctggg ggatctgttc
    22021 aacagggaca acgacatacc acacaatata tttctcatca aattcagtta ccgtttcgct
    22081 ctgaaatagg ttgcctgcct gatccgcatg caaaaattac taatttattc ttctgaatat
    22141 acatttatta ttctgtcgtt tgaccaccat ggttttgaca gataatggca aattcatctg
    22201 accggggttc cacaataata aaactggtta aatcaatgcc ggataaaacc ctgaaatctg
    22261 aggaaatttt tttcttcatc atttgatttt ttttatatat ttacttttta gccgaagaat
    22321 attttaacct gatcactcct gaaagatatt ttttgaactc acctgaaaca attaattttt
    22381 ttactgttaa cccttaaccc tgaaacgcat ggataaagaa ttaagatcct gttttctgac
    22441 gtgtttggcg gaactaaaag gttcgctaaa aagacgcctt tttatgacca gtctgttaac
    22501 cggcctgctg gtcagtttgc cgcaattgtc cctggccgga ggcgaagaaa atcccggcag
    22561 catggatgac aatccggctg ttgagctgat gcagcagagg gtgaccggaa ccatcactga
    22621 tgcttccacc ggagatcctc ttgtcggagt gaatattgtt gttgaaggaa ctacaacagg
    22681 cacaatgaca gatttcaacg gcaaatacac aattgaagtt cctggccgta atgcagtact
    22741 ttcaatatcc tacataggat atatcgctct gaaggtaaat gtggaaggca ggactgtcat
    22801 tgatgtttca atggaatctg atctcagggc acttgaagag gttgttgtaa ccggttatgg
    22861 aactgttagg aaaagtgatc ttaccggatc tgtcagttca attaaaaccg agcagatact
    22921 tcagcttccc acccagcgag tcgaccaggc tatccagggg agagccccgg gtgtattcat
    22981 tctgaatact gatggttctc caggcggaaa taccatgatc cggatcagag gcagcaactc
    23041 aatccatggc ggcaatgatc ccctgatcat tgttgacggt ctgcagggag ccaatataaa
    23101 tcagctgaat ccgaacgaca ttgcttcaat ggaaatcctg aaagatgctg cagccacggc
    23161 g
//
LOCUS       DAOSPX010000011        22606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2472_length_22606_cov_4.804931, whole genome shotgun sequence.
ACCESSION   DAOSPX010000011 DAOSPX010000000
VERSION     DAOSPX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22606
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2472_length_22606_cov_4.804931"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLZ75_01205"
     CDS             <1..265
                     /locus_tag="PLZ75_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOS71046.1"
                     /translation="EKHRRFASPFAVFILTLIGVSLSSRKVRGGIGMNIGIGLALSFS
                     YIFFQQFASQFSLKGNLSPLVAEWIPNFIYIAIGMVLYKIAPK"
     gene            343..1887
                     /locus_tag="PLZ75_01210"
     CDS             343..1887
                     /locus_tag="PLZ75_01210"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOS71047.1"
                     /translation="MALSSKIREFHEKREKLLLGGGEQTVEKQKAMGKRTARERISAL
                     LDEGSFTEYDLFVKHVAHDFDMGDKSLPGDGVITGTGTIDGSPVAVFAQDFTVAGGSL
                     GLMHSRKITKIMDHALKMRMPLIGINDSGGARIQEGVNSLAGYGEIFFRNTLSSGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVDNISKMFITGPEVIKTVLGEEISMEDLGGARV
                     HSEISGNAHFFAGSEEECFAQIKKLIGFIPRNNTEKAGVLTSAEPLTAHEIDGIVPDD
                     PTLPYDVRDVIKAVADNSDFFEVMENFAGSIVIGFGRIEGRTAGFVANQPLEMAGVLD
                     VDSSDKAARFIRFCDAFNIPVITFVDIPGYLPGADQEHAGLIRHGAKILYSYSEATVP
                     KITVILRKAYGGGYIAMGSRHLRADFVFAWPAAEIAVMGPEGAANIIFRKEIMSSADP
                     EETRRQKIEEYRKKFANPYVAASRGYIDAVIEPSETRKFIAHSLKVSENKEIQLPPKK
                     HGLPPF"
     gene            1944..2261
                     /locus_tag="PLZ75_01215"
     CDS             1944..2261
                     /locus_tag="PLZ75_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOS71048.1"
                     /translation="MKKTSQEGKLNIDGTVYRTRLSRKFIKRAPFSPSDPFLLTSIIP
                     GTILDIMVKPGQTVVKGEALMILEAMKMQNVLKSPAGGTVRMIAVKKGDRVGKGSPLL
                     RIG"
     gene            complement(2264..3082)
                     /gene="ispE"
                     /locus_tag="PLZ75_01220"
     CDS             complement(2264..3082)
                     /gene="ispE"
                     /locus_tag="PLZ75_01220"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOS71049.1"
                     /translation="MITFPAAKINIGLRVISRRHDGFHDIETVFYPVGLRDALEFVVH
                     TCSTGEDEMVVTGLGTGSAPGENLVITALKKMRESYNIPALKIHLHKKIPAAAGLGGG
                     SSDAAFFLKAVNKCFSLGIDNETLRRTALEIGSDCPFFVDPVPSYATGRGEILRPAAS
                     LPPGFRIILAYPGIRISTRDAYNNCIPAKPSISLEEIVKQHPSQWKNLVINDFEQYVF
                     RIHPEIRELKEAFYRAGAVYSSLSGSGSAVYAIFRDSIEIPGHIKPWIIYEGDL"
     gene            3370..4860
                     /gene="dnaB"
                     /locus_tag="PLZ75_01225"
     CDS             3370..4860
                     /gene="dnaB"
                     /locus_tag="PLZ75_01225"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOS71050.1"
                     /translation="MTALPDLGKVPPQAIDMEEAVLGAIMLEKEAVIAILDILKPESF
                     YKDAHRKIFKAISDLSAREYPVDLYTVTEELRAHNELDSAGGPVYLSQLTSKVVSAAN
                     VDFHARIVAQKFIQRELIRVSTEIQTRAFDDTWDVTELLDYSENELFQIAEGNIKREV
                     SPINMVIKEAIREIEEAGKRKDALVGIPSGFSKLDRLTSGWQKSELIIIAARPSMGKT
                     AFALSMARNMAIEHGKKVAVFSCEMSSIQLVNRLIIAETDIPGDKIRNGRLTEEEWKQ
                     LDSRIKKLIQAPIYIDDTPAISIFELRAKCRRLMAQHKLDVVIVDYLQLMSGPDNAGS
                     REQEVSNISRSLKSIAKELNVPVIALSQLNRSVEMRGGSKRPLLSDLRESGAIEQDAD
                     MVVFIHRQEKFGLVTFEDGSSTKGIAEIILAKNRNGPVDDVRLRFREEKAQFVDLEEF
                     DLDMVGEMNGTQSITLGSKMNHDRFRGHADNNFDNGYDYSDEPPFA"
     gene            complement(4949..7732)
                     /locus_tag="PLZ75_01230"
     CDS             complement(4949..7732)
                     /locus_tag="PLZ75_01230"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOS71051.1"
                     /translation="MKRALIISFFIFLLPLISLSQILKGRITNEAGDPVPYSTVFIVE
                     LKQGTTANTKGEYELKLPPGKYLVAFQSLGYSPVFLDIAITGETIVRDVTLPYQFYEI
                     PEVRISASGEDPAYDIMRRAIGMAPYYLNHISSWKAEVYIRGNITFRKIPKLARRSVR
                     MDYGSNTHPGGVPVKEGDVYMMESFNEVEFTAPDNYLQKVISVNSTIPAQDDNITPMD
                     IIKASFYQPVIADMAISPLSPRAFSYYRFRYLGTSLQGDLRVFKIQVIPRMKSQQLFE
                     GSIYILDDLWCLHSLELTNNNIAGKISIEQLYIPVEPDIWMPVSLKLGMEISILGFRA
                     DAGYGSSMKYSSVELNKSMKKPGTGSDRTESPASAANAPKDREIQGQQESPRQRGSQG
                     QQESPGQRGSQGQQESPGQREKIERILEKDELSRRDMMALTRLMNREARGKGSDSSRK
                     DLEIRDKTTRIIENDAGRKDSAFWAEIRPVPLSDAEMRSIRIRDSVKNKRQEPREITG
                     DSMGGNQTGKKGRFATVLKRTASGHTWPVAGGPGFTFGGLVRPDNISFNPVDGFTYGT
                     GFRLSGNFGNSGPAYIAPEMSYAFSRRKLMWRMNGGYSFKGSSTKSLYFRAGIISTDI
                     SSGGSINSFLNTMTSLLLKENHLRLYESGYILAGFRDEIANGLKIDLSAEYDNRKILE
                     NSSNFSLFRKTEDYAPNLPENRFLDAGSGSRHRLDDQIHYGFTAGISWLPRQRYRMNE
                     GVRIPAGSSWPLFGFQWKHGIYRYTEPSEGYRHFDMIRFEASKTHEPGAFSEFSWRIR
                     TGSFLNNSHIPFFDFFHFNSQSFPLLLNDYRDAFYLPRYYSLSTPELFGELHLKYTSP
                     YLALKYLPGFSRTLVRENIILSWLGSRNNPHYSEIGYSLSELFFLGEAGIFAGFDNLR
                     FRNFGIRLILKLN"
     gene            complement(7739..8413)
                     /gene="rsmI"
                     /locus_tag="PLZ75_01235"
     CDS             complement(7739..8413)
                     /gene="rsmI"
                     /locus_tag="PLZ75_01235"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOS71052.1"
                     /translation="MSKLFLVPTPIGNLKDITYRAVEILGSVDIILAEDTRHAVRLLK
                     HYNISRKLLSHHKFNEHHSADPVCDLIRSGKTAALISDAGTPGISDPGFLLVRKCIEK
                     GIPVETLPGPSALIPALVNSGLPSDRFCFEGFLPVRKGRRKKLTSLETEPRTMIFYES
                     PYRLLKTLGEMAEHFGPERQASVSRELSKIFEETIRGTLGFLADHFRNNPPKGEIVVV
                     VSGKKN"
     gene            complement(8406..9287)
                     /locus_tag="PLZ75_01240"
     CDS             complement(8406..9287)
                     /locus_tag="PLZ75_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71053.1"
                     /translation="MDFNATIDLIIRELEEAAKIIEDVENYREVPLLHLELARAKCRN
                     AADVIRMLKDMPEKVYPRKQGTAAGVFPEKPEVPEQAARGRTAGADRKDETVKPEEER
                     YGLIRKTAEATGKDRYGKPEKHEETERGSLIRKPEEHEEDEGKDPARKTAEEMGKDRY
                     GKPEEPDIQKPRTAPGKKLPDKVTIADSFSPRADSVIEKLGVRKEEDDMRSIIKSKPI
                     HSLSEAIGINDKFLFVRELFQGDPGKYTRAISALENSSGFEEARKMILNFAGNDEENE
                     AARQLIDLVKRKFSADE"
     gene            9403..10005
                     /locus_tag="PLZ75_01245"
     CDS             9403..10005
                     /locus_tag="PLZ75_01245"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOS71054.1"
                     /translation="MDILENPAGPGDRRGTIEVITGSMFSGKTEELIRRLRRAQLAGL
                     KVEIFKPSLDRRYSETKIVSHDSRWISSTPVDNAYGILLLAAGVDVVGIDEAQFFDDS
                     IVDVCNQLANNGIRTVVAGLDMNFMAKPFGPMPALLAVAEYITKVHAICMRCGSLANY
                     SYRKSSEDQIVVVGEKEKYEPLCRACYNRTAGSTALKDHI"
     gene            10019..12496
                     /locus_tag="PLZ75_01250"
     CDS             10019..12496
                     /locus_tag="PLZ75_01250"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOS71055.1"
                     /translation="MKFSSSDIAEIISGRLSGDPDLTVTEVLIDSRNFKFSEGVLFFA
                     IRGKNHDGHDFIESLYSRGIRVFVVERLPRDMEKFEGAAVIETSGSVAALQKLAAHVR
                     KSFRPPVIAVTGSAGKTIVKEWLADILGMTASVVRSPRSYNSQTGVPLSVLRLEDKYQ
                     YGIFEAGISLPGEMGRLRDIIDPDIGIITNIGDAHSENFSSLEEKAREKLELFRDTSV
                     LIYCRDHRIIHELISGREVYAGKQIADWSVSSDEAAIRVEKISGDGRQTHIRMGYRGR
                     DHFFSIPFSDRASVDNAVTAACACLVLGTDAEVIRQGLASLVSVAMRMELKAGINNCQ
                     LIEDYYNSDPGSLGMALDYLRAQNNRKTTLILSDFVQSGRDEKELYSEVSGLIRQNGT
                     GRFIGIGPVLSANRSLFGGDPEFYYSTDEFIQRFTPSAFRDETILLKGARKFEFEKIA
                     ALLEQQVHQTVLEINLDAIAHNLNEYRRRIRPGTAIMAMVKAFAYGSGHAETAALLEY
                     HRVGYLGVAYADEGVELRNAGITLPVMVMNPDPSAFELMIRYDLEPEIYSFRSFERFR
                     ETALKYGLVGYPVHIKIDTGMHRLGFMPGDADRLASLVRSSESMKIVSVFSHFAASED
                     PALDHFTHRQAALFTKVAEKLREATGYSFIRHICNSSAIIRFPEYHFEMVRPGIGLYG
                     TGYFNAGDADKPDLKPAGRYMTRISQVKTIPAGEPVGYGCAAVADSDRVIAILPVGYA
                     DGLNRKLGNGNGSLFIKNRRVPIIGNICMDMCMADISGVDAGEGDEAEIFGQGISIAE
                     LAGKCGTIPYEILTSIPPRVKRVFFRE"
     gene            complement(12598..14250)
                     /locus_tag="PLZ75_01255"
     CDS             complement(12598..14250)
                     /locus_tag="PLZ75_01255"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOS71056.1"
                     /translation="MRFSVAQLNYHIGNFTANREKIRRAIQKAREEKSDLVIFSELSL
                     PGYPPHDLLQRDDFIEDCRKALDEVADECRGITAIAGCPVVSDSSGSRKLLNSALVLS
                     EGKILFSAAKTVFSGHDIFDEHRYFSPGRNFRIFPFRGFRMALTVGDDIRSEQFPGSE
                     SGDTENHASPAVYELSGQHPDFIVNISAFPFSYKKTGERISAFRNKAARYGVPLINCN
                     QTGANTELIFDGASVTINGKGDICGMLPFFEEEVRTFSSEDLFRGIHHNDHILPGQHE
                     LVYKALVCGIRDYFRKSGFSRAILGLSGGLDSAVVLCLAAEALGNDRVQALLMPSRYS
                     SEDSVTDALTLAERLNIPYNIISIEKPFRAFEEDLAPLFAGKQPDVTEENIQARIRAI
                     LLMAVANKSGAVLLNASNKSEAAVGYGTLYGDMAGGLSVIGDVYKTDVYSLARHINRN
                     GEIIPENIIARPPSAELRADQLDTDSLPEYSILDPILYQYIEEQKAPAAITGRGITPG
                     IVSDVIGMVRNSEYKRFQAPPVLRISSRSFGQGRIMPLAAKY"
     gene            complement(14259..15065)
                     /locus_tag="PLZ75_01260"
     CDS             complement(14259..15065)
                     /locus_tag="PLZ75_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOS71057.1"
                     /translation="MITRVMEKIIRIQRNEMNHTGIKPSVKGYIVLIVLLCISFRISA
                     QTDYRNTGFSSRRDNFAPSYAYSLDIHFDPVISWFSTNRYLMRNEGAIPGSSIGISRS
                     KNYSPNYSFTSGVSITDAGGKLICLEKKYFRLRNPRHETVAVEAGERIIYRIKYFSVP
                     VGIKLRTGETGYGKIFAEAGLDPKIVLSAKADIPSLNIRDNNAFAEIRAFNLSYHVMA
                     GTEYPVIGNLSAVFGLRFENNFLDFIRNNKNKPGEMVSHKLLSFRLGINF"
     gene            15174..16196
                     /locus_tag="PLZ75_01265"
     CDS             15174..16196
                     /locus_tag="PLZ75_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71058.1"
                     /translation="MRNIIFFTVIFSCLMAAGSACRRNHYKVNTSSIEADVKIKRFEQ
                     ALFSADPAGFDEGLPLIREEYGSFLQLFAYVINAGSTDDPDFPSALLSFATDRLNYEV
                     YEEVMRVFPDMAPYEKELEEAFRHYLYHFPDKAVPSVISCVTGFNSSIIAGDSVIGLG
                     LDRYLGRNSPYYPRLEIYQYVAARMNPFNLVPDCMYGWGASEWDYESMDYPEKNLLSV
                     MLHEGKLKYFEKCMLPGISDTLLFGFTASQMKFCENNERQMWQYLIEHDLLFSTDQFV
                     IRKLTGTAPFTSYFTNESPGRAAVWTGFRIVESAMKKNRDISLGDLMDSRDIQGLLTM
                     AGYDPD"
     gene            complement(16251..16421)
                     /locus_tag="PLZ75_01270"
     CDS             complement(16251..16421)
                     /locus_tag="PLZ75_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS71059.1"
                     /translation="MAYKINEDCTACGTCLDECPVEAISEGDIYKIDPDLCTDCGACA
                     DVCPVEAIHPEN"
     gene            complement(16525..19806)
                     /locus_tag="PLZ75_01275"
     CDS             complement(16525..19806)
                     /locus_tag="PLZ75_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71060.1"
                     /translation="MRNRRLLIIIILACLAPLTVTGQFYNGHQMTFGKNRVQYYDYYW
                     SFYRFENIDCYFNEYGRDLAQYTADYAIRKLDEIEDFFDYNLEKRLVFIIHKNNNEYR
                     ESNVGLVTGDADTYNTGGFSRIIKNKVLLYYEGDHVAYQKQIGASITEVIVNEMLYDA
                     DVRDRISSSSVITIPEWFIKGLANYVSFGWDVETENRVKDGIMTGYYKNINHLEYDDA
                     VYAGQSFWRFIGKEYGDALIPNIIYLTKVYKNIEDGLLYVIGKNVKELMKEWKAFYRN
                     EFADDEAKPDYDGNVIARSKKEQLFQQVKISPDGRYIAYVTNNWGRKRIRIYDQTTGK
                     QKVIFRKEPKYEHITDNTYPVIAWHPSGRILTYINEENAGMVIYFYRTDEKKTEKRNL
                     LYFDKILDFSYSPEGSRLIFSGVKDGITDLYLHTISSGTNEQITRDIADDLNPSFLEG
                     EEDVIIFSSNRLSDTITNLADPWEKVGNTFNLFTYNLSRRDNILTRLTEGQYIERFSP
                     SGNIRKRFTYIGDADGMMNRYSATFDSAISYIDTATHYRYFIRSEALSAYDRNILTQS
                     VDRAEDNYGEVLFNKGKYMIRKGKLSDSPPLSDRERKFSNFRKEKSALLHRADSIEQL
                     RQWLISEDKKRRDTLTKPLYEYYLTSEPIDINHYIFEKEKQNYFEQLWRKDYMNIDLD
                     TGRLKLPLIRIYQTAFYNNYIANQIDFSFLNNTYQVYSGGAPYYNPGINLLTRFGVID
                     LFENYRLTGGFRFSGNFDSNEYLVSAESLKGKLDKQFIFHRQGFFSSNDTSYFKSHTH
                     NIYLTFSKPFTPVLSLKGTVAYRNDRHVMLYSDNMVAVLNNDIISRHWGNLKGELVYD
                     NTRKRNINIYYGTRFKIFGEYYKELNYKMSDMIVIGVDYRTYIRLHRELIWANRFAAS
                     TSYGPTRLVYYLGGVDNWMGNIFNRKAMFDQSIPVNPNMNYGFQALATNMRGFIQNIR
                     NGTSFALINSEIRWPVIRYFAGHPLKSNFLNSFQLVGFGDIGSAWVGRTPWAGENAWD
                     NEVIQSGSVKVTLDSNREPIVAGFGAGLRAQLLGYFVRADWAWGIENNYILPRVFYLS
                     FSLDF"
     gene            20069..21157
                     /locus_tag="PLZ75_01280"
     CDS             20069..21157
                     /locus_tag="PLZ75_01280"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOS71061.1"
                     /translation="MKKVCLIITTFFITVSVMAQPVMKLSETEHNFGSFREEDGRQTF
                     DFEVTNTGNSPLVIQNIIASCGCTLPDWTKHPIAPGGKGKITAIYDPQNRPGAFNKTL
                     SVYTNSKPQVVVITLKGEVIPREKTMEELFIFPVGPVRFQSNHLAFTNVKKTEKKIRV
                     MQIINPSKESVKVEFENVPEHLTLKASPETLKPGQKGIIEGAYDATKNNAWGNVSDMV
                     RVKLNGVTQQNVFYYISANLVEDFSSLSKSDLENAPVFKLEATTVDLGKMAGSTSKDV
                     EFRFSNAGKRDLIIRHVRSTCGCTAIQQGTQGEGIRPGQSSSIKATFNSGGYKGRVTK
                     AIYVYTNDPKNSEVVLMLNADVEQTASR"
     gene            21255..22394
                     /gene="meaB"
                     /locus_tag="PLZ75_01285"
     CDS             21255..22394
                     /gene="meaB"
                     /locus_tag="PLZ75_01285"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOS71062.1"
                     /translation="MAGGDKSVGEKPDGKRPDGEQSGSRLRIKAGTEYPAGVNPAVAG
                     GSRRKRKQYSAAEYLDGILKGSRTILSQAITLVESSLPEHAGMMRDLVESCLPFSGKS
                     VRVGITGVPGAGKSSFIEALGLHLVSEGRKVAVLAVDPSSSTSRGSILGDKTRMEKLG
                     TSPDAFIRPSPAAGSAGGVARKTREAIILCEAAGFDTILVETVGVGQSETAVHSMVDF
                     FLLLVLAGAGDELQGMKRGIMEMADAVAVTKADGSNRSRAEHAVRMYRNVLSLFPADP
                     SGWKPVALSCSVRENTGIRELWDVIRKYVALTGENGFFTEKRKLQSVMMMNETIAEYL
                     NNSFYSNDEIRALKLLLEKELLEGRISSNKAALSLIEKYQKIHCR"
BASE COUNT         5630 a         5128 c         5639 g         6209 t
ORIGIN      
        1 agaaaaacac cggcgctttg ccagtccctt tgccgttttc attcttacac tcataggtgt
       61 ctccctgtcc tccaggaaag tgaggggagg tattggcatg aacattggca taggactggc
      121 acttagcttt tcgtatatat ttttccagca gtttgcatcc cagttttccc tcaagggaaa
      181 tctcagtccc ctggtagcgg aatggattcc gaattttatt tacattgcaa taggaatggt
      241 gctttacaaa atcgcaccga aataatgcgg tttatcattc aatttgttta aacttgcata
      301 cctgaagctg agcttgcagc attatcatca atatgattca caatggcatt aagcagcaag
      361 attagggagt ttcatgaaaa gcgtgagaag cttttactcg ggggaggaga acagaccgtc
      421 gaaaagcaga aggccatggg taagcgtact gcccgggagc ggatcagtgc tcttctggat
      481 gagggttcct tcaccgaata tgatcttttt gtgaaacatg tggctcatga ttttgacatg
      541 ggtgacaaat cacttccggg tgacggtgtt attacgggta cgggtactat tgacggatcc
      601 cctgtggcag tgtttgcaca ggattttacc gttgccggcg gttccctggg actgatgcat
      661 tcgaggaaaa tcaccaagat aatggatcat gccctgaaga tgaggatgcc gcttataggg
      721 ataaatgatt caggcggcgc cagaatccag gaaggtgtga attcacttgc aggctacggc
      781 gagatatttt tccgtaacac cctgtcaagc ggggttattc cccagatttc cgtaattctg
      841 ggtccctgtg cgggaggtgc cgtatattca cctgccctga ccgattttgt gttcgtggtt
      901 gacaatatct caaaaatgtt tattaccggt ccggaagtta taaaaaccgt gctgggggaa
      961 gagatctcga tggaagatct gggaggagca cgggttcaca gtgagatcag cgggaatgcc
     1021 catttctttg ccggcagtga ggaagaatgc tttgcccaga taaagaaact catcggtttc
     1081 atacccagga acaatacgga gaaagccgga gtactgacat ctgcagaacc tctgacagct
     1141 catgagatag acgggatagt accggatgac ccgaccctgc catacgatgt aagggatgtt
     1201 ataaaggctg ttgccgacaa ttccgatttt tttgaagtga tggagaattt tgccggaagc
     1261 attgtaatcg gcttcggtcg catagaaggc cggacggccg gttttgtggc caaccagcct
     1321 ctcgagatgg caggtgtact ggatgtggat tcatctgaca aggcagcccg ttttatccgt
     1381 ttctgtgatg ccttcaacat accggtcatt acttttgtgg atatcccggg ctatctgccg
     1441 ggggccgatc aggaacatgc ggggcttatc cgtcatggtg ccaaaatact ctattcctac
     1501 agcgaggcta ccgtgcccaa gatcaccgtg atcctgcgaa aagcctacgg cggaggatat
     1561 attgcaatgg gttcgcggca tctcagggcc gactttgtct ttgcctggcc tgctgccgag
     1621 attgcagtaa tgggacccga aggagcagcc aatattattt tcaggaagga aataatgtcg
     1681 tccgctgatc ctgaagaaac gcgcaggcag aaaattgagg agtacaggaa aaagtttgcg
     1741 aatccctatg ttgcagcatc aaggggctat attgatgcgg ttattgaacc atccgaaaca
     1801 aggaagttca ttgctcattc attgaaagta tcggaaaaca aggaaataca acttccgccg
     1861 aagaagcatg gcctccctcc gttctgataa atcttttgtg acagacacct gctatgctgc
     1921 tgatccggta ataaatgatt gaaatgaaaa agacaagtca ggaaggaaag ctgaatattg
     1981 atggtactgt atacaggacc aggctgagca gaaaattcat caaaagggcc cctttcagtc
     2041 cgtccgatcc ctttttgctt acaagtataa ttccggggac aatactggat atcatggtaa
     2101 agccggggca gactgttgta aaaggagagg cgctgatgat ccttgaagcc atgaagatgc
     2161 agaatgtgct gaaatccccg gcagggggga cagtcagaat gatagcggta aaaaagggtg
     2221 acagggttgg gaaaggaagt cctttgctcc gtatagggtg aactcagaga tcaccctcat
     2281 aaattatcca gggttttata tgcccgggaa tctcaatgct gtccctgaaa atagcatata
     2341 ccgctgatcc gctgcctgac aatgagctgt aaaccgcacc tgccctgtaa aaggcttctt
     2401 tcagttccct gatttcggga tgaatcctga atacatattg ttcaaaatca ttaattacca
     2461 ggttcttcca ttgcgatgga tgctgcttta caatttcttc gagacttatg gagggttttg
     2521 ccgggatgca gttattgtat gcatcccttg tggagatgcg tatccccgga tatgccagta
     2581 taattctgaa tcccggcgga agggatgcgg caggccttag tatttcaccc cttcccgttg
     2641 cataagaggg gaccggatca acgaagaagg gacagtcgct tccgatttca agtgcggtcc
     2701 ttctgagggt ttcattatca attcccaggc tgaagcattt attgactgct ttcaggaaga
     2761 aggcagcatc tgatgatccg ccgcccaggc ctgcagcagc gggtattttt ttgtgaagat
     2821 gtattttcag ggccgggata ttgtaactct cacgcatctt tttcagagcc gtgataacca
     2881 ggttttcccc tggtgccgac ccggtaccga ggccggtgac aaccatttcg tcctccccgg
     2941 tactgcaggt atgtacaaca aattccaggg catcacgtaa gcccacggga taaaataccg
     3001 tctcaatatc atgaaaaccg tcatgacgcc tggagataac ccttaatccg atattgatct
     3061 tggcagccgg gaaagtaatc atatcagctt attttttata aaattataat aatttatctt
     3121 tcactcagtc tttttcagac tccctgccgt gatatttaat agccgtaaaa gtagccggca
     3181 aatgcgggat acttttttca acaagttttt aacagctgat gtttgtaata aaatctgttc
     3241 tttcaggggg gaaatacctt gtaattttta cctttgaagc ctgggggaat tttttgcaaa
     3301 ttcttttaaa tcaattgata ataagcagtt taaagcatgg caaaacagac aaggatcagt
     3361 aggccggctg tgacggcatt accggatctg gggaaggtgc cgcctcaggc cattgatatg
     3421 gaagaggctg ttctgggcgc aataatgctg gaaaaggaag cagtgattgc aatacttgac
     3481 attcttaaac cggaaagttt ttacaaggat gcccaccgga agatattcaa ggcgatatcg
     3541 gatctgtctg ccagggaata tccggttgat ctctatactg tgaccgagga actcagggct
     3601 cacaatgaac tggatagtgc aggcggtccg gtatatcttt cccagctgac ctccaaggtt
     3661 gtttcggcgg caaatgtgga tttccatgca cggatagtgg cgcagaaatt tattcagaga
     3721 gagcttataa gggtatccac ggaaatacag acccgggcat ttgacgacac ctgggatgta
     3781 acggagcttc ttgattattc ggaaaatgaa cttttccaga ttgccgaggg aaacataaag
     3841 cgtgaagttt ccccgatcaa tatggtaata aaggaagcga tacgggagat tgaggaagcg
     3901 ggaaagagaa aagatgccct ggtaggcatt ccttccggat tttcaaaact cgacaggctt
     3961 acttctggct ggcagaagtc cgaactcatc attatagcag ccaggccttc aatggggaaa
     4021 acggcttttg ccctttccat ggcaagaaac atggctattg aacacggtaa gaaggttgct
     4081 gttttcagct gcgagatgtc gtcgatacaa ctggtaaaca ggcttattat agcggagacg
     4141 gatattccgg gagacaagat cagaaacgga aggctaacgg aggaggaatg gaagcagctg
     4201 gacagcagga ttaagaaact gattcaggct cccatctata ttgatgatac tccggcaatc
     4261 tcaatatttg agctcagggc aaagtgccgc cggctgatgg cccagcacaa gctggatgtt
     4321 gttatagtcg actatcttca gcttatgtcg ggaccggaca atgcaggcag ccgtgagcag
     4381 gaggtaagca atatctcccg ttcgctcaaa agcattgcca aggagcttaa tgtgccggtg
     4441 attgcattgt cgcagctcaa ccgttccgtt gagatgaggg ggggatcgaa gcggcccctg
     4501 ctttccgatc tgagggaatc gggagcaatt gagcaggatg cagatatggt ggtattcatt
     4561 caccggcagg agaaattcgg ccttgttaca tttgaagacg gatcatccac aaagggtata
     4621 gctgaaatca ttcttgccaa gaaccgtaac ggccccgtgg acgatgtcag gctcaggttc
     4681 agggaggaga aagcccagtt tgttgatctg gaggagtttg acctcgacat ggtgggagaa
     4741 atgaatggca cgcaaagcat aacgctggga tcaaaaatga atcatgaccg tttccgcgga
     4801 catgcggaca ataattttga caacggatat gattacagcg atgaaccccc gtttgcatga
     4861 attcatgatg gttcttccgg attcctgtta aatcctgaac tgctgatcat ttttgcatgt
     4921 tccggcagaa gactgtatgg ttgatacttc agtttaattt cagaatcagt ctgatcccga
     4981 aatttctgaa tctgagatta tcgaatcctg caaagatccc tgcttcaccc aggaaaaaga
     5041 gttccgacaa actgtatcct atttcactgt aatgaggatt attccggctg ccaagccagg
     5101 aaaggattat attttccctg acaagtgtcc ggctgaatcc gggaagatat ttcagtgcca
     5161 ggtagggact ggtatatttc aggtgcagtt ccccgaacaa ttccggtgtg ctcagtgaat
     5221 aataccgcgg aaggtaaaaa gcatcccggt agtcattgag caatagcgga aatgactggc
     5281 tgttgaaatg aaagaaatca aagaagggaa tatgtgagtt attgaggaag ctgcctgttc
     5341 ttattctcca gctgaattcg ctgaatgctc ccggctcatg tgtttttgat gcttcaaacc
     5401 tgatcatgtc aaaatgcctg tatccctctg aaggctcagt atacctgtat atcccgtgtt
     5461 tccactgaaa accgaataga ggccaggaag atcctgccgg gattctgaca ccctcattca
     5521 tccggtatct ttgccggggc agccagctga tgccggcagt gaatccgtag tgtatctgat
     5581 cgtcaagccg gtgccgggag ccggaaccag catccagaaa gcggttttca ggcaggttgg
     5641 gtgcataatc ctctgtcttt ctgaaaaggg aaaagttact gctgttctca agaattttcc
     5701 tgttgtcata ttctgccgac aggtctattt tcagtccgtt tgctatctca tcgcggaatc
     5761 cggcaagaat atatcctgat tcatagagcc tgagatgatt ttccttcagc agcagtgatg
     5821 tcatcgtgtt caggaaactg ttgatgcttc cgccggatga gatatcggta ctgatgatcc
     5881 ctgccctgaa atacagggat tttgttgatg agcccttgaa gctgtatccg ccattcattc
     5941 tccacatcag ctttctgcgg ctgaaggcat agctcatttc cggtgcgata tatgcaggac
     6001 cgctgttccc gaagttgcct gacagtctga aaccggtacc gtaggtgaat ccgtcaacgg
     6061 gattgaagct tatattgtcc ggcctgacga gtccgccgaa agtgaatccc ggtcccccgg
     6121 caaccggcca ggtgtgtccg gatgcagtcc ttttcagcac agtggcaaac ctgccttttt
     6181 tccccgtctg attcccgccc atgctatccc ctgttatttc ccggggttcc tgccttttgt
     6241 ttttcacgct gtcacggatt cttatactcc gcatctcggc atcagagagg ggaacaggtc
     6301 ttatttcagc ccagaaggca ctgtctttcc tgccggcatc attttcgatt atacgtgttg
     6361 tcttatccct tatttcaaga tctttccgtg atgagtcaga gcctttcccg cgggcttccc
     6421 tgttcatcag gcgggtcagt gccatcatgt cgcgcctgga gagctcatct ttttcaagga
     6481 ttctttcaat tttttcccgc tgccccgggc tttcctgctg tccctggctt ccccgctgcc
     6541 ccgggctttc ctgctgtccc tggcttcccc gctgcctcgg gctttcctgc tgtccctgga
     6601 tttcccggtc cttcggggca ttggccgcgg atgccggtga ttcagttctg tcactgcctg
     6661 ttcccggttt tttcatggac ttgttcagtt ccacggagga atacttcatt gaactgccat
     6721 atccagcatc tgccctgaat cccagaatgc ttatttccat tccgagtttc aggcttaccg
     6781 gcatccagat atccggctcc accggtatat aaagctgttc gatgctgatc tttccggcaa
     6841 tgttgttatt tgtgagctca aggctgtgca ggcaccacag gtcgtccagt atgtagatgc
     6901 tgccttcaaa cagctgctgg ctcttcatcc tcggaatcac ctgtatctta aacaccctga
     6961 ggtcaccctg gagtgaagtt cccagatacc tgaagcggta ataggaaaaa gcccggggag
     7021 acaggggaga aattgccata tccgcgataa caggctgata gaaactggcc tttatgatat
     7081 ccatgggagt tatgttgtca tcctgagcgg gaattgtact gttcacggag atgaccttct
     7141 ggaggtaatt gtcgggtgca gtgaattcca cctcattaaa tgattccatc atgtaaacgt
     7201 ccccttcctt taccggaaca cctcccggat gtgtgttgct gccataatcc attctcacgg
     7261 agcggcgtgc cagttttgga attttcctga atgttatgtt tcccctgata tatacttctg
     7321 ccttccatga actgatgtgg ttaaggtaat agggagccat tccgattgcc cttctcatga
     7381 tatcatatgc cggatcctca ccggaggcgg aaatcctgac ctccggtatt tcatagaact
     7441 ggtagggcag ggtgacatcc ctgacaatgg tttcgccggt aatggcaata tccagaaaaa
     7501 caggtgaata ccccagactc tgaaaagcaa ccaggtattt gccgggagga agtttcagct
     7561 catattcgcc tttggtattg gcagtcgttc cctgtttgag ctcaactata aaaacggtgg
     7621 aataggggac gggatcaccg gcttcatttg ttatcctgcc tttcaggatc tgtgaaaggg
     7681 agatcagcgg aaggaggaat atgaaaaagg aaattatcag ggcacgcttc atccggactc
     7741 agtttttttt tcctgacact acaacaacta tctcgccttt tggcggattg tttctgaaat
     7801 ggtctgcaag aaatccgagt gttcccctga ttgtttcctc aaaaatcttg ctcagttccc
     7861 tggatacgga tgcctgacgc tcaggaccga aatgttctgc catttccccg agtgttttca
     7921 gcagccggta gggagattca taaaaaatca ttgttctcgg ctcggtttca agggaagtca
     7981 gttttttcct tcttcccttc cttacgggca gaaaaccctc aaagcagaac cggtcggacg
     8041 gaagtcccga attgaccagg gcgggaatca gtgcagaggg acccgggagt gtttccaccg
     8101 gaattccttt ttcaatgcat ttccttacaa ggaggaatcc gggatcagag atgccgggcg
     8161 tgcctgcatc tgatatcagt gcagctgttt tccccgatct gatcaggtcg catacaggat
     8221 cagctgaatg gtgttcattg aacttatggt gtgaaagcag tttccggctg atgttgtagt
     8281 gtttcagcag cctgacggca tgccttgtgt cttctgcaag tattatgtcg acgcttccca
     8341 gtatttcaac cgccctgtag gttatatcct tcagattgcc gataggtgtc ggtacaagga
     8401 ataatttact catcagccga gaattttcgt tttacgaggt cgataagttg tctggcagct
     8461 tcgttctcct catcatttcc ggcaaaattc agtatcattt tcctggcttc ctcaaagccc
     8521 gaggagtttt ccagagcgga aatcgccctg gtgtatttcc ccggatcccc ctggaaaagt
     8581 tccctgacga aaaggaattt atcatttatt ccgatggcct ccgataagct gtggatgggt
     8641 ttggatttta ttatacttct catgtcatct tcttccttcc ggactcccag cttttcaata
     8701 acgctgtcag cgcggggact gaaggaatca gctatggtga ctttgtcagg cagttttttt
     8761 ccgggcgcgg tccggggttt ttgtatatct ggttcttccg gctttccgta cctgtctttt
     8821 cccatttctt ctgccgtttt tcgtgccggg tcttttcctt cgtcttcttc atgttcttcc
     8881 ggcttccgga tcaggcttcc tctttctgtt tcttcatgtt tttccggctt tccgtacctg
     8941 tcttttcccg ttgcttctgc cgttttccgg attaagccat acctttcttc ttccggcttt
     9001 acggtttcat ctttcctgtc tgctcctgct gtcctgcctc tggctgcctg ttcagggact
     9061 tccggttttt cggggaaaac acctgctgcc gtgccctgct tccgcgggta aaccttttca
     9121 ggcatatcct tgagcatgcg gatcacatca gcggcattcc tgcactttgc cctggccagt
     9181 tccagatgca gcaggggtac ttcccggtaa ttttccacat cctcaatgat tttcgcagct
     9241 tcttcaagtt ccctgatgat cagatcgatt gttgcattga aatccatgac tcttattgtt
     9301 attaaaacaa atcaattaac ttactttgca aaagtaatta taattccacc ttcataacgg
     9361 gttatataca tactttggta caaaagcaca gatattaagc ctatggatat ccttgaaaat
     9421 ccggcgggcc cgggtgacag gcgcggaacg attgaggtga taaccggttc catgttttca
     9481 gggaaaacgg aggaacttat acgcaggttg cggagagccc agctggcagg gctgaaagtt
     9541 gaaattttca agccttccct ggacaggcgc tattctgaaa caaaaatcgt atcgcatgat
     9601 tccaggtgga tcagctccac tcccgtggac aatgcatatg gtatactgct tcttgccgct
     9661 ggtgttgatg ttgtcggaat tgatgaggcc cagtttttcg atgattccat tgttgatgtc
     9721 tgcaatcagc tggcaaacaa tggcataaga actgtcgtgg ccggtctgga tatgaatttc
     9781 atggccaaac cctttggccc catgcctgca cttcttgccg tggctgagta tattaccaag
     9841 gttcatgcca tatgtatgcg ctgcggcagc ctggcaaatt attcatacag gaaatcctca
     9901 gaggatcaga tagtggtagt tggcgaaaag gaaaagtatg aaccgctgtg cagggcctgt
     9961 tacaacagga cggcaggcag cactgcctta aaagaccata tttaaacaaa caataaatgt
    10021 gaaattctct tcttccgata ttgctgaaat aatttccggc aggctgagcg gcgatccgga
    10081 cctgacggtg acagaagtgc ttattgacag ccgtaatttc aagttcagtg agggagtgct
    10141 gttttttgcc atcaggggta aaaatcatga cgggcatgat tttattgaaa gtctgtacag
    10201 cagggggata agggtttttg ttgttgaacg gcttccccgc gacatggaga aatttgaagg
    10261 agcagctgtc atagagacat ccggttccgt tgcggcttta caaaaacttg ccgcccatgt
    10321 caggaagtca ttccggccgc ccgttattgc cgtgaccggc agtgccggaa aaaccattgt
    10381 aaaggagtgg ctggcggata ttctcggaat gactgcatca gtggtcagaa gtccgcggag
    10441 ctataattcc cagacaggag tccctctttc cgttctcagg cttgaagaca agtatcagta
    10501 cggaatattt gaagccggta tttcattacc gggtgagatg ggcaggctca gggatatcat
    10561 agatcccgat atcggtataa taacgaatat aggtgacgcg cacagtgaga atttcagcag
    10621 ccttgaggag aaggcccggg agaaactgga actcttcagg gatacatcag ttcttattta
    10681 ctgcagggat caccggatta tccatgaact gataagcggc agggaagttt atgcggggaa
    10741 gcagattgcc gactggtcgg tcagcagtga tgaggcagcg atcagggttg aaaaaatttc
    10801 cggagacggc aggcagacac atatacggat gggataccgg ggcagggacc actttttttc
    10861 catacccttc agtgaccggg catctgttga caatgccgtc accgcagcct gtgcctgcct
    10921 tgtcctgggg acagacgcgg aagttatacg gcagggactg gcctcgctcg tatcagttgc
    10981 aatgaggatg gaactgaaag ccgggataaa caattgtcag ctcattgaag actattacaa
    11041 ttccgatccg ggttcccttg gcatggctct ggattatctc agggcgcaga acaacaggaa
    11101 aacgactctc atactttccg atttcgttca gagcggaagg gatgaaaaag aattgtattc
    11161 cgaagtttcc ggactgatac ggcagaacgg aacgggcagg ttcataggga tcgggcctgt
    11221 actttccgcc aaccgttcat tgttcggcgg tgatccggag ttttactatt caacggatga
    11281 gtttatacag cgctttactc cctcggcctt ccgggatgag acaatactac tgaagggagc
    11341 gaggaaattt gaatttgaaa agattgctgc ccttctggaa cagcaggtac atcagacagt
    11401 gctcgagata aaccttgacg ccattgccca taacctgaat gaatacagaa ggagaatcag
    11461 accggggaca gccattatgg cgatggtaaa ggcttttgcc tatggttcgg gccatgcgga
    11521 aacggcagcc cttctggaat accacagggt aggctatctc ggcgttgcat acgcagatga
    11581 gggcgtcgag ctgaggaatg caggaatcac tcttcctgtg atggtaatga atcctgaccc
    11641 ctcggcattt gaactgatga tcaggtatga ccttgaaccc gagatataca gtttccgctc
    11701 attcgaaagg ttcagggaga cagctttgaa atacggactg gtgggttatc ccgtgcatat
    11761 caagattgat acgggaatgc accggcttgg ttttatgccc ggtgatgctg acaggctggc
    11821 aagcctggtg agaagcagtg aaagcatgaa aatcgtatct gttttttccc attttgcagc
    11881 cagtgaggat ccggcccttg atcatttcac tcacaggcag gccgcactgt ttacaaaggt
    11941 ggctgaaaag ctcagggaag caacgggcta ctccttcatc aggcatatat gcaattcatc
    12001 cgcgatcata cgtttccccg aataccattt tgaaatggtc aggcccggca taggcttgta
    12061 cggtaccgga tatttcaatg ccggagatgc tgacaagcct gatcttaaac cggcaggaag
    12121 gtatatgacc aggatatcgc aggtgaaaac aattccggca ggagaacctg tcggttacgg
    12181 atgtgccgct gttgcggaca gtgaccgtgt tattgccata ctgcccgtgg gatatgccga
    12241 tggactgaac aggaagctgg gaaacgggaa tggctcactt ttcataaaga accggagggt
    12301 tcccattatc ggcaatattt gcatggatat gtgcatggcc gacataagcg gagtggacgc
    12361 cggagaaggg gatgaggccg agatattcgg tcagggtatc agcattgccg aactggccgg
    12421 gaaatgcggg accataccct atgagatcct tacatcaata ccaccgcggg taaaaagagt
    12481 ctttttcagg gagtagggaa gagggaatct gtatgatgac ccgtccggag agggcggaac
    12541 agcagtcctt tcctgacaaa atggaggcag aaactgaaag ggccggcaga agggttctca
    12601 gtattttgct gccaggggca ttattcttcc ctgtccgaat gatctggagg aaattctcag
    12661 aaccggcggg gcctggaatc tcttatactc gctgttcctg accattccta tgacatctga
    12721 tacgattccg ggtgttatgc ctctgccggt gattgcggcc ggtgctttct gttcttcaat
    12781 atactggtaa agtattggat ccaggatgct gtattccgga agtgaatcgg tgtcgagctg
    12841 atcagccctc agctctgccg agggtggcct ggcaattata ttttccggaa ttatttcccc
    12901 gtttctgttt atgtggcggg caagactgta aacatcagtt ttgtagacat ccccgatgac
    12961 ggacaggcct cctgccatgt ccccgtataa tgttccgtat cccacggctg cctcactctt
    13021 gttggaagca ttcagtaaaa cggctccgga tttatttgcc actgccatca gaagtattgc
    13081 ccttattctt gcctgaatat tctcttccgt gacatccggc tgtttaccgg caaacagggg
    13141 ggcgagatct tcctcaaagg ccctgaaagg cttttctatg cttatgatgt tgtatggaat
    13201 gttcaggcgt tccgccagtg tgagggcatc ggtaacggaa tcctccgatg aatagcggga
    13261 gggcatcagg agagcctgta ccctgtcatt tccgagcgct tcggctgcaa ggcagagcac
    13321 aaccgccgaa tcaagacccc cggagaggcc caggattgcc ctgctaaacc ctgattttct
    13381 gaaatagtca cgtattccgc ataccagggc tttatataca agctcatgct gaccggggag
    13441 tatatgatca ttatgatgta ttccccggaa taaatcttca gaactgaagg ttctgacctc
    13501 ctcctcaaaa aaaggaagca tgccgcagat atcccctttg ccattgattg tcacggatgc
    13561 accgtcgaag ataagctcgg tgtttgctcc tgtctggttg cagtttataa gcggaacacc
    13621 gtacctggca gctttattcc tgaatgcgga aatcctttca ccggttttct tatatgaaaa
    13681 ggggaaggcc gaaatgttga caatgaaatc aggatgttgc cctgacagtt catacacagc
    13741 cggagaggca tggttttccg tatctcctga ttctgatccg gggaattgtt cactccgtat
    13801 gtcgtcaccg actgtaagcg ccatcctgaa gcccctgaac ggaaagatcc ggaagtttct
    13861 gccgggactg aaatatctgt gctcatcgaa tatgtcgtgt ccggaaaaca ctgttttagc
    13921 cgcagaaaag aggatttttc cctcagacag gacaagggcg gaattaagaa gcttcctgct
    13981 gccggagctg tcactgacaa cagggcagcc ggcaattgcg gttatccccc tgcattcatc
    14041 cgcaacctca tcaagtgctt tcctgcagtc ttctatgaaa tcatcacgct gtagcaggtc
    14101 atggggagga tatcccggca gggaaagctc cgaaaatatt acaagatctg atttttcttc
    14161 ccttgccttt tgtattgccc tgcggatttt ttccctgttg gcagtaaaat tgccgatatg
    14221 gtagttcagc tgtgcgaccg aaaatctcat atgaaatctc agaagttaat ccccagccgg
    14281 aaagatagca atttatgact taccatttca cccggtttat tcttattgtt ccgtatgaaa
    14341 tcaaggaaat tgttttcaaa gcggagtccg aagacggcag agaggtttcc gatgacagga
    14401 tattctgttc cggccattac gtggtatgaa aggttgaagg ccctgatttc agcaaatgca
    14461 ttattatccc gtatattaag agacgggatg tcggctttgg ccgaaagcac gattttcggg
    14521 tccagtccgg cttcggcaaa tattttcccg tagccggttt cgcctgttct tagttttatg
    14581 ccaaccggaa cggagaaata tttaatcctg taaataattc gttcgcctgc ttcaactgcg
    14641 actgtttcgt gccggggatt cctgagcctg aaatattttt tttccaggca gatgagtttt
    14701 ccccctgcgt cggtgatact gactccggat gtgaaggagt agttcgggct gtaatttttg
    14761 ctccgggata tgcctatgct tgaccccggt attgcgcctt cattgcgcat cagatatctg
    14821 tttgtggaaa accagcttat tacgggatcg aaatgaatgt cgagcgagta tgcatacgag
    14881 ggagcgaaat tatcgcgccg ggagctgaaa ccggtgttcc ggtaatcagt ctgggcggaa
    14941 atccggaatg atatgcataa cagaacaatc agtacaatat atcctttgac tgatggtttt
    15001 atacctgtat gattcatctc attcctttgt atgcggatta ttttctccat cacacgggtg
    15061 atcattcatt ttcattatca aatgatcaaa tactgagcca gatttattgc tgcacggatt
    15121 tttgccatca caatgaaaag taattacttt cgtattttca aatttataac ggcatgagaa
    15181 acattatatt cttcacggta attttttcat gtttaatggc agccggaagc gcctgcagaa
    15241 gaaatcatta taaagtgaac acttcatcca tagaggctga tgtaaagata aaaaggtttg
    15301 aacaggctct cttttcggcc gatcctgccg ggtttgatga gggattgcct ctgataaggg
    15361 aggaatacgg cagtttcctg cagttgtttg cctatgttat caatgccgga agcacagatg
    15421 atccggattt tccttccgcg cttctgagct ttgccaccga caggctgaat tatgaagtat
    15481 atgaggaagt aatgcgggtt tttcctgaca tggctcccta tgagaaggaa cttgaagaag
    15541 cattcaggca ttatctctat cattttcccg ataaggcggt tccttcggtt atcagctgtg
    15601 ttaccggttt caacagcagt atcattgccg gcgattcggt tatcggttta gggcttgaca
    15661 ggtatcttgg aaggaacagt ccctattatc cgcgtcttga aatttatcag tatgtggctg
    15721 ccaggatgaa tcccttcaac cttgttcctg actgcatgta cggctggggc gcttcggaat
    15781 gggattatga gagcatggac tatcctgaaa aaaacctgct gagtgtcatg cttcatgaag
    15841 gcaagctgaa atattttgaa aaatgtatgc ttcccggtat aagtgacaca ctgctgtttg
    15901 gtttcactgc ctcccagatg aagttctgcg aaaacaatga aaggcagatg tggcagtacc
    15961 ttatagaaca cgatcttctt ttcagcactg atcagtttgt tatccgcaag cttaccggaa
    16021 cggcaccttt caccagctat ttcacgaacg aatcaccggg aagggcagcg gtctggacag
    16081 gattcagaat agttgaatca gccatgaaga agaaccgtga catcagtctt ggtgatctta
    16141 tggattcaag ggatatacag ggcctgctga cgatggcggg atatgatccc gactgaaaca
    16201 aaacgggact gaattgcttc agccccggca gttaatctga atacacagca tcagttctca
    16261 ggatgaattg cctcaaccgg gcagacatcc gcgcatgctc cgcagtcggt gcataagtcc
    16321 ggatctattt tgtagatgtc gccttcagaa attgcctcaa ccggacattc gtccaggcat
    16381 gttccgcacg ctgtgcaatc ttcattgatt ttgtaagcca tttttatttt agtattagaa
    16441 tttttgagtt tccaaatgta tccaaaaaaa ttttatttta aaatgccgca tgacacatag
    16501 cagcggcatg tcttccggat aaggtcagaa atcgagactg aatgacaggt agaagaccct
    16561 tgggagaatg tagttgtttt caatgcccca tgcccagtcg gctcttacga aatatcccag
    16621 cagctgggct cttaatcctg ccccgaatcc tgccactatc ggctcccggt ttgagtcaag
    16681 tgtcactttc acggaaccgc tctggattac ttcgttgtcc catgcatttt ctcccgccca
    16741 gggtgttctt cccacccatg cggatccgat gtctccgaaa ccgaccagct ggaaggagtt
    16801 gagaaagttt gatttaagcg ggtgtcctgc aaagtacctg attacgggcc atcttatctc
    16861 actgtttatc agggcaaagc tcgtgccgtt gcggatattc tggataaaac cgcgcatatt
    16921 tgtagccagg gcctggaagc catagttcat attgggattg acaggaattg actggtcaaa
    16981 cattgctttc ctgttgaaaa tatttcccat ccagttatcc acgcctccaa ggtagtaaac
    17041 aagtctggtt ggtccgtagg atgtgcttgc cgcaaagcgg tttgcccaga taagttccct
    17101 gtggagtctg atataggtac ggtagtccac acctatcaca atcatatctg acattttgta
    17161 attcagctcc ttgtagtatt cgccaaagat cttgaagcgg gttccgtaat aaatatttat
    17221 atttcttttc cgtgtgttgt catataccag ttcccccttc aggtttcccc agtggcggga
    17281 aatgatatca ttgttgagca cggcaaccat gttgtcggaa taaagcatga cgtggcggtc
    17341 atttctgtag gcaacggtac ccttgagcga aagtacgggc gtaaagggct ttgaaaaggt
    17401 caggtagata ttgtgagtat gcgatttgaa atagctggta tcatttgaac tgaagaatcc
    17461 ctgccggtgg aatatgaatt gtttgtcgag tttccccttc aggctttcag cgcttaccag
    17521 gtactcgttg ctgtcgaagt tgcctgagaa ccggaatcct cctgtcaaac ggtaattttc
    17581 gaagaggtcg attaccccga atctggtaag caggtttatc ccggggttat agtagggagc
    17641 tcccccgctg tatacctggt aggtgttgtt caggaagctg aagtcgatct gatttgctat
    17701 gtaattgttg tagaaggctg tctggtagat tcttataaga ggcagtttaa gccgccccgt
    17761 atcaagatca atattcatgt aatcctttct ccagagctgt tcgaaataat tctgtttttc
    17821 tttctcgaag atatagtggt taatatctat gggctcactg gtcaggtaat attcatacag
    17881 cggttttgta agggtatcgc gtcttttttt gtcttcggaa ataagccatt gcctgagttg
    17941 ttcaatactg tctgccctgt ggagcagggc agatttctcc tttctgaagt tgctgaattt
    18001 tctttcacga tccgacaggg ggggggagtc ggacagtttt cctttccgga tcatgtattt
    18061 tcccttgttg aacagcacct ccccgtaatt atcctctgcc ctgtccactg actgggtgag
    18121 gatattgcgg tcataggcgc tgagggcttc ggatctgata aaatacctgt aatgggtggc
    18181 ggtgtcgata tagcttatgg cactgtcgaa ggtggcggag taacggttca tcataccatc
    18241 ggcatcgccg atgtaggtaa atctctttct gatatttccc gaaggggaaa atctctcaat
    18301 gtattgccct tccgtcagcc tggtgagaat attatccctc cgggaaaggt tgtaggtgaa
    18361 aagattgaag gtgtttccga ctttttccca gggatcggca aggttggtaa tagtgtcgct
    18421 gagcctgttt gatgaaaaga tgatcacatc ttcctcccct tccaggaaag aaggattgag
    18481 gtcatctgct atatccctgg tgatctgttc gtttgttcct gaagagattg tatgcaggta
    18541 aaggtcggtt atgccgtcct tcaccccgga aaaaatcagg cgtgatcctt cgggtgaata
    18601 ggaaaaatcc agaatcttgt caaaataaag aaggtttctc ttttcagtct ttttctcgtc
    18661 cgtcctgtag aaataaataa ccattccggc attttcctcg ttaatgtatg taaggatcct
    18721 tccgctcgga tgccatgcta ttacggggta ggtattatct gtgatatgct catatttggg
    18781 ttctttccgg aaaataacct tctgtttgcc tgttgtctga tcatagatcc tgatcctttt
    18841 ccttccccag ttgttggtca cataagcaat atacctgcca tcgggactga tttttacctg
    18901 ctggaagagc tgttcctttt ttgaacgggc aatgacattt ccgtcatagt caggcttcgc
    18961 ctcatcgtca gcgaactcat tgcggtagaa tgccttccat tccttcatca gttcctttac
    19021 attcttgccg ataacataca ggaggccgtc ctcgatattc ttatatacct ttgtcaggta
    19081 tataatattg ggtatcagcg catcgccata ttccttaccg ataaaacgcc agaatgactg
    19141 tccggcataa acggcatcat cgtattctag atggtttata ttcttgtaat agcctgtcat
    19201 gatgccatcc tttacccggt tttccgtttc aacatcccat ccgaatgaaa catagtttgc
    19261 cagtcccttg ataaaccatt ccggtatggt gatcacggat gagctgctga tgcggtcccg
    19321 cacatcggcg tcataaagca tctcattcac tatcacttcg gttattgatg ccccgatctg
    19381 tttctggtag gcaacgtgat ccccctcgta gtagagcagc accttgtttt ttattattct
    19441 gctgaatccg ccggtgttat aggtgtccgc atcacctgtg acaaggccga cattggattc
    19501 cctgtactcg ttattgtttt tatggataat gaaaacgagc cgtttttcga ggttatagtc
    19561 aaaaaagtcc tctatctcat ccagtttcct gatggcataa tcggcggtat actgggcgag
    19621 atcgcggccg tattcgttga aataacagtc aatattctca aaacggtaga aggaccagta
    19681 ataatcataa tactgcaccc tgttcttgcc gaatgtcatc tggtgtccgt tgtagaactg
    19741 tcctgttaca gtcagcggag caaggcatgc aagtatgatt attatcagca ggcggcggtt
    19801 tctcattcct tgtttctttg tgaaacatca gggtttttcg ttcaaatccc gttccaaaac
    19861 cctcttatta tgcaacaaaa ctaatgatat tccggtttaa aatatcttaa aatagtaaag
    19921 tttattaaat ttgtcatgtt taatgtcaaa catcattccg gtctgatttt tgtatcaacc
    19981 agtgcaacca tacaattacc ggggttgttt atatactaaa aaccgtattg gaatgttaaa
    20041 tattataagt gtctaacaat ttcgtaatat gaaaaaagtc tgtctgataa ttacaacgtt
    20101 ttttattact gtttcggtga tggctcaacc ggttatgaag ctttccgaaa ctgaacacaa
    20161 ttttggttct ttcagggaag aagatggtcg tcagaccttt gattttgagg tcaccaacac
    20221 cgggaattcc cctctggtga tacagaatat cattgcctca tgcggctgta cccttcccga
    20281 ctggaccaag catccgattg ccccgggagg aaaagggaag ataaccgcta tctatgatcc
    20341 gcagaaccgg cccggagcat tcaacaaaac tctttccgtc tatacaaact caaaaccgca
    20401 ggttgttgtt attactctga agggagaagt tattccgcgg gaaaaaacca tggaagaact
    20461 gtttatcttt cctgttggtc cggtaaggtt tcagagcaat catctcgctt tcaccaatgt
    20521 gaaaaagacc gaaaagaaaa tacgcgtcat gcagattatc aatccctcga aggaatctgt
    20581 aaaggttgaa tttgaaaatg ttccggagca tctgactctt aaggccagtc cggagacact
    20641 caagcccggc cagaaaggta ttatagaagg tgcctatgac gccacaaaaa acaatgcctg
    20701 gggcaatgtc agcgatatgg taagggtgaa gctcaacggc gtgacacagc agaatgtgtt
    20761 ttattacatt tcggccaatc tggttgaaga tttctcctca ctttccaaaa gcgacctgga
    20821 aaatgcacct gtgttcaagc ttgaggccac gaccgttgat ctgggaaaaa tggccggttc
    20881 cacttccaaa gatgttgaat tcaggttttc gaatgccggc aagagggatc tgataatacg
    20941 tcatgtcagg tcaacctgcg gatgcactgc cattcagcag ggtacccagg gagaaggcat
    21001 caggcccggt cagtccagtt ccataaaggc gaccttcaat tcaggaggct acaagggcag
    21061 ggtaaccaag gccatctatg tctatacgaa tgatccgaag aattccgaag tggttcttat
    21121 gctcaatgcc gatgttgagc agactgcttc aagatgatat gcagatcccg tgatctgatg
    21181 tttaaccctg aaagggtgct gtatgagata acggcaccct tttactgttt ttacagctat
    21241 aaaaaccgtt cagtatggca ggaggagata agtcagtcgg agaaaagcca gacggaaaaa
    21301 ggccagacgg agaacagtcg ggcagccgtc tcaggataaa ggctggaaca gaataccccg
    21361 cgggtgttaa tccggcggtt gccggaggca gcaggaggaa gagaaagcag tattccgccg
    21421 cagaatacct tgatggtata ttgaaaggca gcaggaccat cctgagccag gccataacgc
    21481 tggtcgagag ttcccttccg gagcatgccg gcatgatgcg tgacctggtt gaaagctgcc
    21541 ttccattcag cggaaaatcg gtcagggtcg ggataacggg cgtgcccgga gccggcaaga
    21601 gttcgttcat tgaggccctg ggcctgcatc ttgtttcgga gggccgtaag gtggcggttc
    21661 tggctgttga cccgagcagc agcacttccc ggggcagcat cctgggagac aagacaagga
    21721 tggaaaagct cggcaccagt ccggatgctt ttatccggcc gtctcctgcc gcaggaagtg
    21781 ccgggggagt tgcacggaag acacgtgagg caattatcct ctgcgaggca gccggatttg
    21841 ataccatact ggtggagact gtcggtgtgg gtcagtctga aacggcagtg cattccatgg
    21901 ttgatttctt tcttctgctt gtgcttgcag gtgccgggga cgaattgcag ggcatgaaaa
    21961 ggggcataat ggaaatggct gatgcagttg ccgtcaccaa agccgacgga tccaacagga
    22021 gccgggctga gcatgcggtc cgcatgtacc gtaatgtcct ctcacttttt cccgccgatc
    22081 cctcggggtg gaaacctgtg gctctgagct gctcagtgcg ggagaatacg ggaataaggg
    22141 aattgtggga tgttatacgg aaatatgtgg cacttaccgg cgaaaacggt tttttcacgg
    22201 aaaaacgcaa gctgcagtct gtaatgatga tgaatgaaac aattgcggaa tatctgaata
    22261 attcatttta cagcaacgat gagatccgtg ctttgaagct cctgttggag aaggagctcc
    22321 tggaaggcag aataagttcc aataaagctg ccctgagcct tattgagaaa tatcagaaaa
    22381 tacattgccg ctaaacaata ttttgtatct ttgcgcgatt taaagattaa aaatctgata
    22441 cttaatacat ttagcataat gaagaaaatt atctttttat ccgttctggc agcagttctc
    22501 tctgcctgta cttccggccc ccagtttgtc atcagaggaa agctggccgg tgctgacagt
    22561 gttaccctgg tacttcagaa aagagcagac ggacagattg tgaatc
//
LOCUS       DAOSPX010000012        22174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2528_length_22174_cov_4.428998, whole genome shotgun sequence.
ACCESSION   DAOSPX010000012 DAOSPX010000000
VERSION     DAOSPX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22174
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2528_length_22174_cov_4.428998"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLZ75_01290"
     CDS             complement(<1..570)
                     /locus_tag="PLZ75_01290"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS71063.1"
                     /translation="MKRHLLLQILLSLIFLTDLYPRPVQTEIKTLETGAIAPDFSLRG
                     IDDKIYSLKDFSKSPVLVVIFSCNHCPTAQAYEDRIISFTKECGAKGVAVVMISPNNV
                     DALTYSELGYSDMGDSFEDMKQRAADKIFPFIYLYDGDEQKSALAYGPVATPHCFVFD
                     KDRILRYNGRVDGSEKPGTCNGEDLRNAVD"
     gene            722..1306
                     /locus_tag="PLZ75_01295"
     CDS             722..1306
                     /locus_tag="PLZ75_01295"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOS71064.1"
                     /translation="MANFIDDFMKNYGPEVSRQLSSNLNIDQGTVQKLIPELAPLILG
                     GLKRQKDDRGGDERVNHILNKYGDSSVLGNLKGLISGKLNDSSADANLGGLLGDKGGV
                     AAARALGKKMNIDPSVIMKIIPALAPVILAALTKKRDTGKTGIGGIGSLLDANGDGNI
                     IDDIAGFILKGKGGASAGAGKNILGSLLGGITKK"
     gene            complement(1426..1818)
                     /locus_tag="PLZ75_01300"
     CDS             complement(1426..1818)
                     /locus_tag="PLZ75_01300"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOS71065.1"
                     /translation="MFHKALDIFFLLFHSSLVLFNLFGWIWRKTRFYNLITLGLTGLS
                     WSLLGLIAGVPGYCPLTDWHFSILEKMGEKGLPASYMKYLADRITGLDFSSALVDNLI
                     LYGFLGALTVSLILNIRDCIKTKSPETK"
     gene            2153..3685
                     /locus_tag="PLZ75_01305"
     CDS             2153..3685
                     /locus_tag="PLZ75_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOS71066.1"
                     /translation="MEKLIITGSGLTINDVVSVARHGRRVELHPDARRRISMCREMLE
                     KKLEAHEIMYGINTGIGEFSEIVLDDNQIRDFQKYLVYNHAAGIGDPAPLETVRGAML
                     GRINVHARGQSGNRPEITETLAEMLNRGVTPYVCQKGSVGASGDLAPMSQIALLLLGE
                     GKAYFRGELLSGKEAMDRAGIPVPGLHARDGLATINGSNMLTAMSAIWLVDANNWLKQ
                     AEIAASMSLEALKANMKPYNPLLHEARGFRGAVRSAGAINKCIAEGDLAEGKVKSKVQ
                     DAYSMRSTPQVIGAAHDMLAFARSQVEIELNGVGDNPVFFPDRDLQISGANFQGTPVS
                     VPMDMAGACITMVSVMSERRMNRLNNVALSEGLPDFLTKGAGMFSGMMLSQYTADTLI
                     VEQRILSMPASIQSIPAAADQEDFVSMGMNTAIKNNQILDCAYGILGIEFMAAAQALD
                     FRKSEYKFGRGVQKAKDVIRKHVSFLDIDRPLYPDHTLMKELVKSCEILEEVEKEVGK
                     LE"
     gene            3812..5116
                     /locus_tag="PLZ75_01310"
     CDS             3812..5116
                     /locus_tag="PLZ75_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS71067.1"
                     /translation="MMRKPFFLLIPVLLCSAMISHAQQVYTPSAENLKAREWFQDAKF
                     GMFIHWGVYSILGDGEWVMNNQRIDKETYQKLPAFFNPLGYDPAEWVAIAKNAGMKYI
                     TITSKHHDGFAMFDSKLTDWDIVDRTPYKKDVIKMLADECRKEGIKLFFYHSQLDWYQ
                     EDYYPRGNTGQASGRPDKGNWYSYLDFMDGQLTELLTNYGEIGGIWFDGFWDKRNSDW
                     RLDQTYGLIHRLQPACLVGSNHHLTPFPGEDFQMFEKDLPGQQTTGFNPEQTVGQLPL
                     ETCETMNNSWGFNLQDKNYKSTKSLIQYLVKAAGFNSNFLLNVGPMPDGKIQPEFIST
                     LKEIGEWMKKNGETVYGTRGGPVSPRSWGVTTQKGNKVFVHILNPEDNNILIPDFGKK
                     IRNISAFGSGVRIKYRQDAFGIAITLPDNLIDETDTILVIEI"
     gene            complement(5214..6569)
                     /locus_tag="PLZ75_01315"
     CDS             complement(5214..6569)
                     /locus_tag="PLZ75_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS71068.1"
                     /translation="MKNRTFITVIIMVFLLISPQLSRAQLPKETEAQKMARMKWWTEA
                     RFGMFIHWGLYALPARHEWIMNRERITAEQYRKYFEMFDPDLYNPQEWARMARQAGMK
                     YVVLTAKHHEGFCLFDSKYTDYKSTNTPAKRDLIKEFVEAFRAEGLKVGFYYSLIDWH
                     HPDYTIDRNHPQRQETDAAYEKLNRGKDMNKYREYMKNQVRELLTNYGEISIMWFDFS
                     FPGKNGKGRDDWDSENLLKLARSLQPGIIVDDRLDLRDVEGGWDFTTPEQYKVAKWPE
                     VDGRRVPWETCQTFSGSWGYYRDETTWKSPAQLLELLIESVSKGGNLLLNVGPTARGE
                     FDYRAKERLDAMGDWMKVNSRSIYGCTEAPAGFRTPENTLLTYNPSTNRLYIHLLAYP
                     LGRLTLEDMADRIKYVQFLHDASEISMSAPREGGKNDLSLTLPVLKPPAEIPVLEVFL
                     K"
     gene            complement(6600..7367)
                     /locus_tag="PLZ75_01320"
     CDS             complement(6600..7367)
                     /locus_tag="PLZ75_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOS71069.1"
                     /translation="MKNKTAYFRFYEELNDFLPSGRKQQSFQHSFRGDPSIKDVIESL
                     GVPHTEIDLILVNGRPVDFSYKPGNEDHISVYPVFESFNISGVTRLRQEPLRTLKFIA
                     DVHLGKLVRYMRLLGFDTLFERTYDDREIVELSVSQKRVILTRDRQLLKNRRVTHGYW
                     VRSSDPREQIREIADRFDLRNDLKIFTRCMECNGLLSEISKEEVSADLPPRTRQYYQR
                     FRKCGGCGKIYWEGSHYENMKKEIGNLLNLDREELNL"
     gene            complement(7476..8654)
                     /locus_tag="PLZ75_01325"
     CDS             complement(7476..8654)
                     /locus_tag="PLZ75_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOS71070.1"
                     /translation="MIESFRFSRLPLIIFGRGKSRELPALIRNFGNRMLLVSGRFSAM
                     ENFPGLSFAGVLEEEGIEVEKLVVSGEPSDRLIDDAVSHYRKHPPDLVVSVGGGSVMD
                     AGKAISAMLTVEGNVRDYLEGVGFREHPGTKIPFIAVPTTAGTGSEATKNSVISRVGK
                     DGFKKSLRHDNFVPDTALVDPDLTISCPPEVTAASGMDCFTQLTEAYLSTRSNEYTDA
                     LATEGLKAVRASLTRVFRNGKDSDARTGMAFAALTSGICLANAGLGVVHGFASAAGAL
                     YDIPHGLICGSLMAASNEMTVRRLREQSGSGPFLARYASLARLFIDDAGKKSDERLID
                     EFIEYLYELTAGLNLPGLRQAGISENDLPEIALNTESKNNPVKLDTEDLMAVLQKRYF
                     "
     gene            complement(8654..8956)
                     /locus_tag="PLZ75_01330"
     CDS             complement(8654..8956)
                     /locus_tag="PLZ75_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOS71071.1"
                     /translation="MIVTCVYVHVKPESIGDFIKATEENHNESVKEAGNFRFDFIQQA
                     DDPARFMVYEAYESEKDVEAHKQTAHYLKWRDKVAGMMAEPRKGVRYNILKPDKRE"
     gene            complement(9080..10276)
                     /locus_tag="PLZ75_01335"
     CDS             complement(9080..10276)
                     /locus_tag="PLZ75_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOS71072.1"
                     /translation="MKPKTSFVLGLMMFLQYYVWGSWYVTMGTFMTKFLGSTGIQIGA
                     AYSALAIATMISPFFIGMVADRFFAAQKVMGVLHLIGGVLLFLAARVTTNSTFYWVIL
                     LYSLAYMPTIGLSNSVAFRQMTDPGKQFPLVRVFGTVGWVIAGFMIAALGIEQTTGTF
                     IMAAVVSVALGLFSFILPDTPPPAREPTSVKSIMGVDAFVLFRDKPYLIFFIAAIFVC
                     IPLSFYFGFANLFLNQAGMHNAAGKMVMGQISEALFILAIPFLFNRIGVKNMLLIGMS
                     AWIARYVCFAFGDMGPNAWMLYAGIILHGICYDFFFVTGYMYTEKKSNERIKSASQGL
                     FTFVTYGLGMFIGTWVSGFVTNYYSAGQVYQWQKIWFIPTYIAVAVLVCFIFFFKEKK
                     EIDVAR"
     gene            complement(10398..11594)
                     /locus_tag="PLZ75_01340"
     CDS             complement(10398..11594)
                     /locus_tag="PLZ75_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOS71073.1"
                     /translation="MKVRHVILRQFQIHGPAEPLDSVKTLQADSGAMVDVEPVKIEYD
                     DLTRQKAAEIAGMKDIAAIAPAIPVKLIAPVDREPGDVSTAARDSWGIEAVGADISPF
                     DGSGIVVALLDTGIDPDHPSFEGIELIRKNFTSEADNDIVGHGTHCAGTVFGRNVSGS
                     RIGVAPGIKKALIGKILGKGGSSSDIVTEGIQWAVQNGANIVSMSFGIDFPGYVGQLT
                     GKGIPVDLATSMALEGYRSNVLLFERLAAMIRVLGDFRQPCLLVAAAGNESRRNVNPD
                     YVISVSPPAVAEGMISVAALNKVSDGYTVASFSNTGARVSAPGVGILSAKRGGGLELL
                     SGTSMATPHVAGVAALWAQKLRNENALKGTILTDKLMGSANYEGMKAGFLPADIGSGI
                     VRAPFD"
     gene            11922..12059
                     /locus_tag="PLZ75_01345"
     CDS             11922..12059
                     /locus_tag="PLZ75_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71074.1"
                     /translation="MDTSKNQSSKNPGTTPVQIIAVLITYLIAGFITVCICGIGYLLI
                     K"
     gene            12386..12529
                     /locus_tag="PLZ75_01350"
     CDS             12386..12529
                     /locus_tag="PLZ75_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71075.1"
                     /translation="MKVIYKVTSEPTGIVLIRRRKIAKALRWWLRENGFEFKYYYYFG
                     YVQ"
     gene            complement(12618..12830)
                     /locus_tag="PLZ75_01355"
     CDS             complement(12618..12830)
                     /locus_tag="PLZ75_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71076.1"
                     /translation="MRTLIRFAWIFAGTGVVCLILAAISLIFKTSIIEIDEINFFRAA
                     NSFFLLTIVLLIYADLKLRKRRITFN"
     gene            13296..13511
                     /locus_tag="PLZ75_01360"
     CDS             13296..13511
                     /locus_tag="PLZ75_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71077.1"
                     /translation="MEARAHNPEEQLRKIINLINENKSDTISVRKIRDIIGSEHLSGK
                     KIHNPFAQRKSYMEFNKDDGYRENYIS"
     gene            complement(13646..14833)
                     /locus_tag="PLZ75_01365"
     CDS             complement(13646..14833)
                     /locus_tag="PLZ75_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS71078.1"
                     /translation="MRISIIMIFLLPFLVLSVPAARAQTIPDDILRQIEQNNSTLAAL
                     RKKTDAERLGHKTDIYLPGPQADFNYLNGSPEDIGPRKDMSVRQAFDFPSAYVFRSRI
                     SDLMNDQAELEYLSHRADVFFQVRLVLADLVFLNSMSRIISERCENIRKIYELYEMKY
                     DAGEIGIMDLNKARLNLLGIQSELENNNIETAFSLNELARYNGGNRIAFSDTVYAWYP
                     AGQDFESWFEGARGNNPSLQWIGKEIAAGEVRIRLSRAMNLPRLEAGYMSEKTAGEQF
                     RGLAMGFSLPLWENRNKIRYEKASLLASKEMETDYRLQYHEQMKALYEKILSLQASIE
                     SFRSALKSADNRGYLLKALSGGEISLTEYLLEYGIYYDSFRKLKEMEREKNRSVAELN
                     RYN"
     gene            complement(14830..17922)
                     /locus_tag="PLZ75_01370"
     CDS             complement(14830..17922)
                     /locus_tag="PLZ75_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOS71079.1"
                     /translation="MLDSIIKFSLNNKFLILIASALLLVYGSRTASRMEIDVFPDLTA
                     PTVVVMTDAHGMAPEEVERMVTFPIETAVNGATDVRRVRSASSQGYSFVWVEFDWGTD
                     IFRARQIVSEKLIGISSGMPMGVGQPMLAPQSSVMGEIFFIGIQSDSTSLMDLRTLAE
                     WSVKPLILSTGGVSQVTITGGDYKQYQVLAEPQKMKYFRVTMQELAGAVRGLARNSSG
                     GVLRQYGNEYAIRGIGRTPDLEAIGDSYIKTAAGNIVRISDIAEVRIGSAVKMGHASS
                     NAEPAVIISVSKQPNANTLEVTRQIEKNLESLKNSLPPDVRLDTKIFRQADFIERSVR
                     NVQRALIEGAIFVTIILIVFLGSFRTTLISLLAIPLSLLGAVLVLRLLGMSINTMSLG
                     GMAIAIGSLVDDAIIDVENVYKRLRQNRMKPAGERESSFTVVFTASKEIRASIFNATL
                     IIMVAFFPLFFLSGMEGRMLKPLGISFLVSLFVSLVIAMTLTPLLARMMLSNEKYLER
                     NEKDKWLIRHLTYYYRISLEWALKKKTAILVSTGIVLIATLVVLSGFGRSLLPEFNEG
                     ALTLSILTQPGTSLEENDRLGRLIEKELLALPEVTSTARRTGRGELDEHSQSTNSSEI
                     DVNFLKGKRSQREFVADVRATLAKIPGIVFTVGQPLGHRIDHMLSGTSANIAIKLFGT
                     ELNKMFSTANEIRSVITDIEGLVDVNVEQQIEVPQIQIRANREMLLRHRIPIDEFTDF
                     ADLAFSGEKLGDIYEGQRSFDLVLRLNSDYTESLDGIKTALIDTYDGKKVPLEEVADI
                     VSATGPGSISRENVRRKIVISANVAGRDLRSVVNEIQDNINRRIQLPEDYWIEYGGQF
                     ESEARASRTLLFTSVVALFIIFLLLYLEFRNFRLAGIILLNLPLALIGGVFAIGLTSG
                     VLSIPAIIGFITLFGIATRNGILLISNYQRLALKGVSPGETIIRGSADRLNAILMTAL
                     TAALALVPLALKGDLSGNEIQSPMAKVILGGLLTSTLLNIYIVPIVYSLFKNPKNAGK
                     "
     gene            complement(17934..19433)
                     /locus_tag="PLZ75_01375"
     CDS             complement(17934..19433)
                     /locus_tag="PLZ75_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS71080.1"
                     /translation="MKFRLLYFFISVILLTGCGNKPEDHHSEHHDHMIQYTAYCDRFE
                     LFIETDHFVAGEPASIVTYLSSVPGFRAVGDAKIRLVYIINGKEFVPENTASSGKGIY
                     SFSFVPESAGSGILKYNISAGESECELIVNNVEVYGNHEAVHEALEDGAHSEANTSVF
                     TKEQSWKVDFSTGYPRQEAFAQVIKTTGLVQPLSSNEFIVSARTGGIVQFGPGGIYEG
                     DEVGQGQILFTVSGSSLADNNFSVRYSQAKNNFERSSADMERALSLLKDRIIPEKEFL
                     EIKNRFENDRSVYESYRESFNNTGQLVTSPAKGFIMRIMVSNGEYVNAGQTLVVILQN
                     SFLAVKVDVPQRYSSQLHQIKAVRIRKINAGRTYSLDELGGRILSYGKAAGEDNFLIP
                     VTLGIESHGEFIPGSFVEVFLEAGDREDTLVVPNTAILEEQGVYFVWVQHTPELFEKR
                     EIRTGRTDGYRTEVAEGLRADERIVTEGAIYIKLAQATGALDAHSGHVH"
     gene            complement(19519..19989)
                     /locus_tag="PLZ75_01380"
     CDS             complement(19519..19989)
                     /locus_tag="PLZ75_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71081.1"
                     /translation="MRNKVLKIPLIIILGTMAFLARTVIPHHHHGPFTCFATAHRQPC
                     ACLPSGSLSFDDLQNESESTGSHGNDKGQQGDNCFLELEVTVPEINEKIQIGTFENIT
                     PDQLLNSDYNSLTGEGLLYINQNRPSFLVFERGSGKYQLKDSYPASGLRAPPAA"
     gene            20372..22027
                     /locus_tag="PLZ75_01385"
     CDS             20372..22027
                     /locus_tag="PLZ75_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71082.1"
                     /translation="MFTLITAIPVHAQDLSELNKSEPFTISGSAGLTSSFYSASGIPD
                     RQAPFAYGIHANATMTLYGIAMPFSFTWYNNSKVGFSQPFNQFGISPKYRWLTLHLGY
                     RNLSFSEFTLSGYTFLGAGIEASPGRFRIGAMYGKFNQNSVYDLAMADSMPKMTRTGW
                     AAKLGYGSEKRFVDLSVLRIGDDPKGYDTLNIEPGIPAPEQNIALGLTSRFDISEKLT
                     FRFDGSYSFYTFNRAFAATGISDDALLRLAGNFIRVNSTSEYFKALKTSISYSFSQGT
                     VTGIEYRRIDPGFRSMGSYFFNNDIEHLTINQSLALLKNKINLRGSLGAQRDNLKGAK
                     EHTALRIIGSLTGNYNINQNWAVDATFNNFSTNQRVFRTATDQSLLIYQVNRTITLSP
                     RYMNAGEKMSHMAMLNINVTALEDKNQSTSDMTETETYMAMLIYNLGLLQLKMNISAG
                     LNYTMMLNKNFENRLAGGNINVAKTLLRDQLSLGLNNNLMINRINDDKGLILNSALTA
                     AWRFHPKHSLSLNLNLINNRFAEGSAVPTYHEIRGDFGYAFTF"
BASE COUNT         6140 a         5472 c         4766 g         5796 t
ORIGIN      
        1 atcaactgca ttcctgagat cttccccatt gcaggtcccg ggcttttccg acccgtcaac
       61 ccttccgtta tacctgagta ttctgtcttt atcaaaaacg aaacaatgtg gagtggcaac
      121 cgggccataa gccagggcgc tcttttgttc atcgccatcg taaagataaa tgaagggaaa
      181 aattttatcg gctgcacgct gtttcatatc ctcaaaactg tcacccatat ccgaataacc
      241 cagttcggaa taggtcagag catccacatt gttcggggat atcattacaa cagcgactcc
      301 tttcgccccg cactcctttg tgaaagagat tatcctgtct tcataagcct gtgcagtggg
      361 acaatgatta cagctgaaaa tgacgaccag aacgggcgat ttactgaaat ccttcagact
      421 gtatatttta tcatcaattc cccgaaggct gaaatcagga gcaatggctc cggtctcaag
      481 agtttttatt tcagtctgaa ccggccgggg atataaatca gtcaagaaaa tgaggctgag
      541 caatatctgc aagagtaaat gtcttttcat gatttcccgg gaacatattc aaatatactt
      601 tgtttttact gattggcaga tgcagaatgg agaatataaa caaatactat tttgatccgg
      661 cagtccggat taggattttg tattcaaatc aagtttccaa ataaatcaat ctcaatttac
      721 tatggcaaat tttattgacg atttcatgaa aaactatggt cctgaggtaa gcaggcagct
      781 atcatcaaac ctgaatattg accagggaac cgttcagaag cttattcccg aactggcacc
      841 gcttatcctg ggcgggctca agcgtcagaa ggatgaccgc ggaggagatg aaagggtaaa
      901 ccatatactg aacaaatatg gtgattcgtc ggtgcttggc aacctgaaag gtcttatctc
      961 gggcaaactg aatgacagca gtgctgacgc aaatcttggc ggacttcttg gagacaaggg
     1021 aggtgtggca gcagcccggg cacttggtaa aaaaatgaac attgaccctt cggttattat
     1081 gaagataatt cccgcactgg ctcctgttat ccttgcagcc ctgacaaaga aaagggatac
     1141 ggggaaaacc ggaatcggag gaataggatc actgcttgat gcaaatggcg acggaaatat
     1201 tattgacgat attgcaggct ttattttaaa aggaaaaggc ggtgcatctg caggtgcggg
     1261 taaaaatatc ctcggatcac tcctgggagg cattactaaa aaataagagg cggattccag
     1321 ggtcgcagaa tgaatccagg atcgcagaat gaatcccgga tcgcagaaca aatccccggg
     1381 ttgcagaata aattctccgg ttatagaata aatccccggc ggcagtcact ttgtctccgg
     1441 ggattttgtt ttaatacaat ccctgatatt caggatcaga gaaaccgtta aagccccgag
     1501 aaacccataa aggatcagat tatccaccag agcggaactg aaatccagtc ccgtgatcct
     1561 gtcggcaaga tatttcatat atgaagccgg aaggcctttt tcccccatct tctcaagtat
     1621 actgaaatgc cagtcggtaa ggggacagta acccggaacc ccggcaatca gaccgagcag
     1681 tgaccatgac aatcccgtca gcccaagcgt tataaggtta taaaatcttg tcttccgcca
     1741 tatccagcca aagaggttga acagcacaag tgaggaatga aacagcagga agaaaatatc
     1801 aagagcctta tgaaacatgg catatctgta tttgatccgg aaataaaatc tttcacaaac
     1861 aaaaataaaa gccggtaagt cttacttttc cgaaattata catttttaca aaggcatggc
     1921 agtggtttga cgtggaaaat aagcaggaat tctttaaatt tgttttgcaa atcatgtata
     1981 tgcatcacaa ctggttcctg gaatataaaa tcaagatcaa ccggacaggt ctttgcgggt
     2041 agtatcctca actgatataa ttatcaagat aatcattgtt gaagtcaaac atcttgatta
     2101 tctgattatc tgaaacaatt ccggtataaa tattcttcta taaatcaaat aaatggaaaa
     2161 actaataata acaggctcag gcctcacaat caacgacgtg gtaagcgttg cccgccacgg
     2221 ccggagagtt gaactgcatc ccgatgcgcg caggagaatc agcatgtgcc gcgaaatgct
     2281 ggaaaagaaa ctggaggcac acgaaataat gtacggtatc aataccggta tcggtgaatt
     2341 ttctgaaata gttctcgacg acaatcagat cagggatttt cagaaatacc tcgtttataa
     2401 ccatgctgcc ggcataggtg accctgctcc ccttgaaaca gtaaggggcg caatgcttgg
     2461 aaggattaac gtgcatgccc gcggtcaatc cggaaaccgg cctgaaatca ccgaaacact
     2521 ggcagagatg ctgaacaggg gtgtcacacc ctatgtatgc cagaaaggtt ctgtgggagc
     2581 ttccggtgat cttgccccta tgtcacagat agcccttctc ctccttggtg agggaaaagc
     2641 atatttcagg ggggaactgc tcagcggaaa agaagccatg gacagggcag gtattcccgt
     2701 tcccggactg catgcaaggg acggacttgc aacaatcaac ggatcaaaca tgctgacagc
     2761 catgagtgcc atatggctcg tcgacgcgaa taactggctg aagcaggcgg aaatagcagc
     2821 ctcaatgtcg cttgaggccc tgaaagccaa catgaaaccc tacaatccgc tccttcatga
     2881 agcaagggga ttcaggggag cggtgagaag tgccggggca atcaataaat gtattgccga
     2941 aggtgacctt gctgaaggaa aagtgaaatc aaaagtacag gatgcctatt ccatgagatc
     3001 aacaccccag gttataggag cggcacatga catgctcgcc tttgcaaggt cccaggtaga
     3061 aattgaactg aacggcgtgg gtgataatcc ggtctttttc cccgacaggg atcttcagat
     3121 ctcaggtgcc aacttccagg gcaccccggt aagcgttccg atggatatgg ccggtgcctg
     3181 tatcacaatg gtcagcgtaa tgtcggaaag acggatgaac cgtctaaaca atgttgccct
     3241 cagtgaaggc ctgccggatt tcctgacaaa gggcgcaggg atgttctcgg gaatgatgct
     3301 tagccagtat acggccgaca ccctgatagt tgaacaaagg atactgtcaa tgccggcttc
     3361 gatacagtct attccagcag ctgccgacca ggaggatttt gtttcaatgg gaatgaatac
     3421 tgcaatcaaa aacaaccaga tacttgactg cgcatacggt atcctgggca tagagtttat
     3481 ggcagcagcc caggccctgg atttcagaaa gagtgaatat aaattcggca ggggcgttca
     3541 gaaagctaag gatgtcatca gaaaacatgt cagcttcctg gatatcgacc gccctctcta
     3601 tcccgatcat accctcatga aagaactcgt gaaatcctgc gagatacttg aggaggtgga
     3661 aaaggaagtg ggaaagctgg aatgatttcc tgaccgccgg atggtgcagt tgacagcagg
     3721 gctatgctgg ttctgaacac gccatataca ggcctcacgg gtcatgcacg gataaatatt
     3781 ttacaattta ttaatacaaa atttcatgat catgatgcga aaaccatttt ttttactgat
     3841 tccggtactg ctgtgttcag caatgatatc ccatgcccag caggtctaca caccttccgc
     3901 tgaaaacctg aaagcccgtg aatggtttca ggatgcaaaa ttcggaatgt tcatccactg
     3961 gggtgtttat tcaatactcg gagatggtga atgggtaatg aacaaccaga gaattgacaa
     4021 ggagacatac cagaagcttc ctgccttctt caacccgctg ggctatgatc ccgctgaatg
     4081 ggttgcaatt gcaaaaaatg caggtatgaa gtatatcacc attacaagca agcatcacga
     4141 cggattcgcc atgtttgatt caaagctcac tgactgggac atagttgacc gtacacccta
     4201 caaaaaggat gtcataaaga tgctggccga cgaatgcagg aaggagggaa taaagctttt
     4261 cttctatcac tcccagcttg actggtacca ggaagattat tatccacggg gcaatacagg
     4321 ccaggcctcg ggtcgtcccg acaaaggcaa ctggtacagc tatctcgatt tcatggacgg
     4381 acagcttacg gaactcctta caaattacgg tgaaataggc ggtatatggt tcgacggttt
     4441 ctgggacaag agaaactccg actggaggct tgatcagaca tacggtctga tacacaggct
     4501 tcagcccgca tgtctcgtgg ggagcaatca tcacctgact cctttcccgg gtgaagattt
     4561 ccagatgttc gaaaaggacc tgccgggcca gcaaaccacc ggtttcaatc ccgaacagac
     4621 agtggggcag cttccgctgg aaacatgtga aacaatgaac aattcctggg gattcaacct
     4681 gcaggataaa aattataaat caacaaaaag ccttatccag tatcttgtaa aggcagcagg
     4741 cttcaattca aactttctgc tcaatgtggg tcccatgcct gacgggaaga tacagcccga
     4801 atttatctcg accctgaagg aaatcggcga atggatgaag aagaacggag aaactgttta
     4861 cggaacgcgg ggcggtccgg taagtcccag gtcatggggt gtgactacac aaaaaggaaa
     4921 taaggtattt gtgcacatac tcaacccgga agacaataat atcctcattc ccgatttcgg
     4981 caaaaaaatc cggaatataa gtgcctttgg ctcgggggtc aggataaagt acaggcagga
     5041 tgctttcggc attgccatta ccctgcctga taatcttata gacgaaacgg atactatact
     5101 ggttatcgaa atctagttca cggctcaatc tttccctgaa gacctgctgt gccggcctga
     5161 ggcagaatac tccgtaacaa ggaatgcggc ccctgaaaag gagctgcatt tccttatttc
     5221 agaaacacct caagcaccgg gatttctgcc gggggcttaa gtaccggaag cgttagcgac
     5281 aggtcattct tgccgccttc ccggggggct gacatgctta tttctgaagc atcatggagg
     5341 aactggacat attttatcct gtcagccata tcttcaagag tgagtcttcc gagcggatag
     5401 gcaagcaaat gaatatagag cctgtttgtt gacggattat atgtcagaag agtgttttca
     5461 ggcgtcctga atcctgccgg tgcttcggta catccgtaaa tggaacggct gttcaccttc
     5521 atccagtcgc ccatggcatc cagtctttcc tttgccctgt aatcgaattc gccccttgcc
     5581 gtcgggccta cattcagaag caggttgcct cccttgctta ctgattctat gagtagttca
     5641 agcagctggg ccgggctctt ccatgttgtc tcatcacggt aataacccca cgatccggag
     5701 aaagtctggc aggtttccca gggtactctc cggccgtcaa cttcaggcca ttttgcaacc
     5761 ttatactgct caggggttgt aaaatcccat cctccctcaa catctcgcag atcaaggcgg
     5821 tcatccacaa taatgccggg ctgaagtgaa cgggccagct tcagcaggtt ttccgaatcc
     5881 cagtcatcgc gacccttgcc gttttttccg ggaaatgaga aatcaaacca cataatgctt
     5941 atttccccgt aattggtaag caactccctt acctggttct tcatatattc ccggtatttg
     6001 ttcatgtcct ttccccggtt cagtttttca tatgcggcat ccgtttcctg ccgctgcgga
     6061 tggttcctgt caatcgtata gtcgggatga tgccagtcaa tcagtgaata ataaaaaccg
     6121 accttcaacc cctccgccct gaatgcctcg acaaactctt ttatcagatc cctttttgcc
     6181 ggtgtattgg tcgatttata gtcagtgtat ttcgaatcga acagacagaa tccttcatga
     6241 tgctttgcgg tcagtacaac atacttcatc cctgcctgcc tggccatcct ggcccattcc
     6301 tgaggattat aaagatccgg atcaaacatc tcgaaatatt tccggtactg ctccgctgtg
     6361 attctctccc tgttcattat ccattcatgc cttgccggca gggcatacag gccccagtga
     6421 ataaacattc cgaaacgtgc ttcagtccac catttcattc tcgccatttt ctgcgcttca
     6481 gtttctttcg gaagctgtgc ccgcgaaagc tgaggggata tgagcagaaa aaccataatt
     6541 ataactgtga taaaggttct gttcttcata ctttcctttt tttgaattaa tactgtggtt
     6601 tataaattta attcttccct gtctaaatta agcaaattcc cgatttcttt tttcatgttc
     6661 tcataatggg agccttccca gtagattttg ccacatcccc cgcatttgcg gaaacgctgg
     6721 taatattgcc tggtccttgg agggagatct gcagacactt cctccttgct tatttctgaa
     6781 agcaggccgt tgcattccat acatctggta aaaatcttca gatcatttct caggtcaaat
     6841 ctgtccgcta tctcccttat ctgttccctc gggtcggaag accggaccca gtatccgtga
     6901 gtgacacgcc tgtttttaag cagctgcctg tcacgggtaa gtatcacccg cttctgggat
     6961 acggaaagct caacaatctc cctgtcgtca taagtccgtt caaacagagt gtcaaagcca
     7021 agcagtctca tatatcttac cagtttgccc agatgcacgt cggcgatgaa tttcagcgtt
     7081 ctcagaggtt cctgcctgag acgggtgacc ccactgatat taaagctttc aaaaacggga
     7141 taaacggaaa tatgatcctc atttccgggt ttataggaaa aatcaaccgg tctgccgttc
     7201 acaagtatca gatcaatttc cgtatgagga acgcctagcg actcaataac atcctttatt
     7261 gagggatctc ccctgaatga atgctgaaat gactgttgtt tcctgccgga tggcagaaaa
     7321 tcattcagtt cctcataaaa ccggaaatat gctgtcttgt ttttcatttt tctgattccg
     7381 gacgttcctg ctcattctcc tgaaatgaac ctggtgaacc aggaaacccg gagttgctga
     7441 ataccgtgga aacatcatga tccttgcgga aaagtttaaa aatatctttt ctgaagtaca
     7501 gccatcagat cttccgtgtc cagtttcaca ggattgttct tggattcggt gttcagagct
     7561 atttccggca gatcgttctc actgataccg gcctgcctga gcccggggag attcagtccg
     7621 gccgtcagtt catagagata ttcaatgaat tcatcaatca gccgctcatc acttttcttc
     7681 ccggcatcat ctatgaaaag ccgtgcaagc gatgcatatc tggcgaggaa gggtccgctg
     7741 ccggattgct ctctcagccg tctcaccgtc atctcattgg aagctgccat aaggctgccg
     7801 cagataagtc cgtgcggtat atcatacaag gctcctgccg ctgaggcaaa accatgaacg
     7861 actcccagtc cggcattagc aaggcagata cccgaggtaa gtgctgcaaa agccattccc
     7921 gttcttgcat cactgtcctt tccattccgg aagaccctgg tcagggatgc cctgactgcc
     7981 ttcagccctt cggtcgccag ggcatcagta tattcatttg accgggttga aagatatgct
     8041 tcggtcagct gggtaaaaca atccattcct gaagcagcag taacttccgg cgggcatgaa
     8101 atggtcagat caggatcaac cagggctgta tcgggaacga aattgtcatg ccgcagggat
     8161 tttttgaagc catccttccc cactcttgaa attactgaat tctttgttgc ttcactgcct
     8221 gttccggctg tcgtcggcac ggcaataaag ggaatttttg ttccgggatg ctctctgaaa
     8281 ccgacccctt caaggtagtc ccttacatta ccttcaacag tcagcatggc agaaattgcc
     8341 tttccggcat ccatcacact cccaccgccc accgaaacaa caaggtcagg cggatgtttc
     8401 ctgtaatgac ttactgcatc atcaatcagc ctgtcggatg gttcaccgct gacaacaagc
     8461 ttctccacct caatcccttc ttcttccaga actcctgcaa agctgagtcc gggaaaattc
     8521 tccattgccg aaaatctccc ggaaacaaga agcatcctgt tcccgaaatt tctgataagg
     8581 gcaggcagct cccgggattt tcccctgccg aaaattataa gcggcagtct gctgaaccgg
     8641 aatgattcta tcattattcc cgcttatcgg gtttcagaat attgtatctg actcccttac
     8701 ggggttccgc catcataccg gcaactttgt cgcgccattt cagatagtga gcagtctgct
     8761 tgtgtgcttc cacatccttt tccgattcat aggcttcata aaccataaac ctggccgggt
     8821 catccgcctg ctggataaaa tcaaacctga aatttccggc ttcctttacc gattcattat
     8881 gattttcttc agttgctttt ataaaatccc cgatcgactc gggtttaaca tgtacataaa
     8941 cacaggtaac aatcatttct cgtattttta agtaaaaata agttttctgt cataaaaacc
     9001 cattgtatca gcacatcgtc acgctaccgg gactcttgcc ggaaatcagg gtccgggcag
     9061 cctggtctgc aacaggcaat tatctggcaa catcaatttc cttcttttcc ttgaagaaaa
     9121 agatgaaaca aacaaggacg gcaaccgcaa tataggttgg aatgaaccat atcttctgcc
     9181 actggtaaac ctggccggca ctgtagtaat tggtcacaaa gccggaaacc caggtgccaa
     9241 tgaacattcc cagcccgtag gtaacaaagg tgaatagtcc ctgtgaagca ctctttatcc
     9301 tctcattcga ctttttctcg gtgtacatat agcctgtcac aaagaaaaag tcatagcaga
     9361 tcccatgaag aattattccg gcataaagca tccaggcatt cggtcccatg tcgccgaatg
     9421 caaaacagac atacctggct atccatgccg acattccgat aagcagcatg ttcttgacac
     9481 ctattctgtt gaaaaggaaa ggtattgcca ggatgaaaag ggcttccgaa atctgaccca
     9541 tcaccatttt accggcagca ttatgcattc ctgcctgatt aagaaacagg tttgcgaatc
     9601 cgaaatagaa ggaaaggggt atgcagacaa atatggcggc tataaaaaag atcagatagg
     9661 gcttgtcgcg gaaaagtaca aaagcatcaa cacccattat tgattttact gaggtgggct
     9721 ccctggcagg aggcggagta tcgggaagaa taaaactgaa taaaccaagt gcaactgaaa
     9781 ctactgcggc catgataaat gttccggttg tttgttcgat gcccagtgca gctatcataa
     9841 atcctgcaat aacccagccc acggttccga acaccctgac aagtggaaac tgcttgcccg
     9901 gatctgtcat ctgcctgaat gccacactgt ttgacagacc tatcgtaggc atgtaggcaa
     9961 gcgaataaag aaggataacc cagtaaaaag tactgtttgt ggttaccctg gctgccagga
    10021 aaagtaatac gccgccaatc aggtggagta ctcccataac tttctgggcg gcaaaaaatc
    10081 tgtcagccac catgccaata aaaaacgggg agatcatggt tgcgattgcc agtgcagaat
    10141 aggcagctcc tatctgtatg ccggtggaac caagaaattt ggtcatgaat gttcccatcg
    10201 taacatacca tgatccccat acgtagtatt gaagaaacat catcagtccg agaacaaagc
    10261 tggtttttgg tttcataatc agtagatttc aggttttttt actcaaattg gatatgtttc
    10321 cggagctgtt cctgtgtaca gagtacttcc ggtgctttaa aattaaaaaa ttgaatcaga
    10381 ataaaaaacg gttttaatca gtcaaagggc gccctgacaa ttcccgaacc gatatccgcg
    10441 ggtaagaatc cggctttcat gccctcatag ttagcagaac ccatcagttt atccgtgagt
    10501 attgtccctt tcagggcatt ttcatttctg agtttctgtg cccagagtgc agccacgcct
    10561 gcaacatgag gcgttgccat actggttccg ctcaacagtt caaggcctcc gccccgtttt
    10621 gccgataata ttccgactcc cggtgctgac acccttgctc cggtattgga aaaagaagca
    10681 accgtataac catctgatac tttgttcaat gctgcgaccg aaatcattcc ctcagccaca
    10741 gccggcggac tgacggaaat tacatagtcc ggattcacat tccggcggct ttcattgcct
    10801 gctgcggcaa caagaaggca gggctggcga aaatctccca gcacccttat cattgcagca
    10861 agccgttcaa aaagaagcac attgctcctg tagccttcaa gtgccattga agttgcaaga
    10921 tccacaggta tcccctttcc tgtaagctga ccaacatagc ccgggaaatc aatgccaaac
    10981 gacattgaga caatatttgc accattctga acagcccact gaattccctc cgtgactata
    11041 tcgctgctgc tgccgccttt tcccagtatt tttccaataa gggctttttt aattccggga
    11101 gccacaccta tccgggatcc ggatacattt ctcccgaaaa cagttccggc acaatgggtt
    11161 ccgtgaccaa ctatatcgtt gtcagcctct gaggtgaaat ttttccttat cagttcaatt
    11221 ccctcgaaag agggatgatc cggatcaatt cctgtatcca gcagggcaac cacgatgcct
    11281 gaaccatcaa agggagatat gtctgccccg acagcttcta tcccccagga atcccttgca
    11341 gccgtggata catcacccgg ctccctgtcc acgggagcaa tcagtttcac cggtatcgcc
    11401 ggagcaatgg cggcaatatc cttcattcct gctatctcag cagctttttg cctggtaaga
    11461 tcatcatatt caatctttac aggttcaaca tcaaccatgg ctccggaatc agcctgcaag
    11521 gtcttaacgc tgtcaagagg ttctgccggc ccgtgaatct gaaactgacg taaaataaca
    11581 tgcctgactt tcattttttg tatagatttt ctgattctgt tatgtgataa attctttcaa
    11641 tactccccca ccccggttat gcaatttaca gcataggccg ataaaaatca acaatatacc
    11701 gggtcaatca gcggatgtta ataaaaattc atttttattc ataatttgaa acaatacata
    11761 aataaagaat gcgtttatat ttaacatgaa atgatgaata taattatttt attactattt
    11821 ttgatccttg aatgtgtctg atacaaattg cataattaat gaataattat acaccgaatc
    11881 gtttgctcaa ctgatcctga accgttttta atgtctgaat tatggataca agtaaaaacc
    11941 aatcttcaaa aaacccggga acaacacctg tccagatcat tgccgtactg ataacctacc
    12001 ttatcgccgg ttttataaca gtatgcatct gcggtatcgg ctaccttctg ataaaatagc
    12061 caggatacca tcacggtatt ccggccgttg gtcaaaacag gcaaaaaacc ggccctgact
    12121 gcagattgaa caattctcat tgattgtcaa cggattgcat tatcctttag catccaatcc
    12181 ccttttttcc gttttttcct gtcaatttac gccaggtttt gacgaatgta cccccgggat
    12241 ctataagagg catgttaaac caaacaagcg gtcatcattg tttgacaaag gcttttcaaa
    12301 tcatgctgaa aagtccatat aacgggcgtt tgcagcttgt tttaaggctc tccgcaatgt
    12361 atctttgaaa gaaaagttat ataaaatgaa agtcatttac aaggttacat ctgagcctac
    12421 aggaattgtc cttatcagga gaagaaagat tgcaaaagca ttaagatggt ggctgcgtga
    12481 gaacggattt gaattcaagt attactatta tttcggatat gttcagtaag caatggccac
    12541 tgaaaagctg caattcctga atacaggatc tgccggcccc gtcccggcag cctgcagccg
    12601 tctgcttcag aaaatcatta attgaaggtt atccttcttt ttcttaattt gagatccgca
    12661 taaatcagga gtacgattgt cagaagaaaa aagctgttgg ctgccctgaa aaaattgatt
    12721 tcgtcaattt caataatgga agttttgaat atcaatgaaa ttgctgccaa tatcagacaa
    12781 acaactcctg ttccggcgaa aatccatgca aaacgtatta gtgttctcat aacctgaagt
    12841 tttaaaaaag aaccttaatt catgccgctt cacctgctgc agttatccct ggcttcatct
    12901 gcacatcact cagaatccgg gactgtaccc ggaaatcatg ttcccgaatg taaagatact
    12961 atatgatttt ttgtttctaa ctgtctattt tattgatttt gaccaatata gaataatact
    13021 ggattaaacc tgacaggcag gtgacaaaaa cagggtaaat aaaaggaaga atgtatccgc
    13081 cccttcaggc aggagcatag gaaagacttg acttttattg aaattattag taattttata
    13141 cctgggttga aagtcaaaac tccgtcattt tctcccttgg agacccgctt gtatacaagg
    13201 gcttttaaac aggcctgatt tatattcata gctccaaaaa aggggaagcc gaaaaccttg
    13261 aaaagagtag gcataatagc aaaaggaagt ctttcatgga agcacgagcc cataatccgg
    13321 aagaacagct ccggaaaatt atcaatctga taaatgaaaa taaatcagat accatcagcg
    13381 tccgcaaaat aagggatatc atcggcagtg aacatctctc cggaaaaaaa atacacaatc
    13441 cctttgctca gagaaaatcc tacatggaat tcaataaaga cgacggctac agagagaatt
    13501 acatttccta gcagttccgg cgattacgat acacaaacat atgaaactga aaagccttca
    13561 ggtttcacca ggagaattgt tatcagctca gcataacaat aacagaatct ttttgcccga
    13621 aaagcgtcaa acagccccgc cggtatcagt tatacctgtt taattccgcg acagatctgt
    13681 ttttttccct ttccatttcc ttcagcttcc tgaaactgtc ataataaatt ccatattcga
    13741 gtagatattc cgtcagggat atttcacccc cgctgagtgc tttcaggagg tatcccctgt
    13801 tgtctgccga tttaagggct gagcggaaag attctatgct ggcctgcaaa gacaggattt
    13861 tctcataaag tgccttcatc tgttcatggt actgcaacct gtaatctgtt tccatttcct
    13921 ttgaggcaag aagacttgcc ttttcatatc tgatcttgtt cctgttctcc cagagaggca
    13981 gtgaaaaacc catggcaagt ccccggaact gttccccggc tgtcttttca ctcatatatc
    14041 ctgcttcaag cctgggcaga ttcatcgccc ggctgagccg gatccgtact tcccccgcag
    14101 ctatttcctt tccgatccac tgcagtgagg gattgttccc ccgggcacct tcaaaccatg
    14161 attcaaaatc ctgacctgcc ggataccagg cataaaccgt gtcagagaaa gcaatcctgt
    14221 ttccgccgtt atatctcgcc agttcattta gcgaaaaggc ggtttcaata ttgttgtttt
    14281 ccagctcgct ctgtatgccc agcaaattaa gtcttgcctt gttcagatcc attatgccaa
    14341 tttctccggc atcatatttc atttcataca gttcatagat cttcctgatg ttttcacagc
    14401 gttccgaaat gatccgcgac atggaattca ggaaaacaag gtcagccaga acaagcctga
    14461 cctgaaaaaa cacatcagcc ctgtgactca ggtattcaag ctctgcctga tcattcatca
    14521 ggtctgaaat cctcgaccgg aaaacatatg ctgaaggaaa atcaaatgcc tgccgtacgg
    14581 acatatcttt tctgggacca atatcttcag gagagccatt aaggtaattg aaatctgcct
    14641 gggggccggg aagataaata tccgttttat gaccaagcct ttcagcatct gtttttttcc
    14701 gcaaggcagc cagtgtactg ttattctgtt ctatctgtcg gagaatatca tcaggaatgg
    14761 tctgggcccg ggctgccggt accgacagaa caagaaatgg caataaaaat atcatgatta
    14821 ttgaaatcct cattttcctg cattttttgg gtttttgaaa agactgtata ctattggtac
    14881 tatatagata ttcaacaggg ttgatgtcag cagtccgccc aggataacct ttgccatcgg
    14941 actctgtatt tcatttccag aaagatctcc cttgagggcc agcggaacca gtgcaagggc
    15001 agcagtcagg gcggtcatca gaatcgcatt aagcctgtcg gccgatcccc ggataattgt
    15061 ctccccgggt gatactccct tcaatgccag tctctggtaa tttgaaatca gaagaatgcc
    15121 attccgggtg gcaatgccga aaagggtgat aaagcctatt atggccggaa tgcttaaaac
    15181 ccctgatgta agcccgatgg caaatactcc gccgataagg gcaagaggca gattcagaag
    15241 gatgattcct gcaagcctga aattcctgaa ctccaggtaa agcagaagga aaattataaa
    15301 cagggccacc actgaggtga acaacagagt ccgggatgct cttgcctcac tttcaaactg
    15361 acctccgtat tcaatccagt aatcctcagg aagttgtatt ctcctgttaa tattatcctg
    15421 aatttcattc accacactcc tgagatcgcg tccggccaca ttggctgata taacaatttt
    15481 tctcctgaca ttttcccggc tgatggaacc ggggcctgtt gccgaaacaa tgtccgcaac
    15541 ctcttcaaga ggaactttct ttccgtcata ggtatcaatc agggcagttt ttataccatc
    15601 cagagattca gtataatcac tgttaagcct gagtaccagg tcaaaacttc tttgcccctc
    15661 ataaatatca cccagtttct ccccactgaa ggcgagatcg gcgaaatcag taaattcatc
    15721 aatcggtatt ctgtggcgaa gaagcatttc cctgtttgca cgtatctgaa tctgtggcac
    15781 ttctatctgc tgttcaacat ttacatccac caggccctca atatctgtta taaccgacct
    15841 gatttcattt gccgttgaaa acattttatt cagttcagtg ccaaacagct ttatcgcgat
    15901 attggcactc gtacccgaca acatgtgatc gatacggtgc ccgaggggct gaccgacagt
    15961 aaagacaata ccgggtatct ttgccagagt tgcccttaca tcagccacaa attcccgctg
    16021 cgaacgcttt cccttaagga aatttacatc aatttccgag ctgttcgttg actgggaatg
    16081 ttcatcgagt tctccccttc ccgtacggcg ggccgtactt gtaacctcag gaagtgcgag
    16141 aagttccttt tcgataagtc tgccaagccg gtcattttct tccagggaag tacccggttg
    16201 tgttaatatg gaaagggtaa gggcaccctc attgaactca ggaagtaaac tgcgcccgaa
    16261 tcctgacagt acaaccaggg ttgctatcag aacaattccg gttgagacaa gtattgcagt
    16321 cttcttttta agcgcccatt caagtgatat cctgtaatag tatgttaagt gccgtatcag
    16381 ccatttatcc ttttcattcc tttcaaggta cttttcattc gaaagcatca tcctggctag
    16441 cagcggtgta agtgtcatgg caatcacaag cgacacaaac agcgaaacaa ggaatgagat
    16501 gccgagaggt ttcagcattc ttccctccat acccgacaga aagaacaatg gaaagaaagc
    16561 aaccattatt ataagggttg cattgaagat agatgccctg atctcctttg aggctgtaaa
    16621 aacaactgta aacgatgatt ccctttcgcc ggccggcttc attctgtttt gccggagcct
    16681 cttatataca ttctccacat ctatgatggc atcatcaaca agtgatccta ttgcaatagc
    16741 cattccgccc agactcatgg tattgataga catacccagt aatctcagca ccagaacagc
    16801 accaagaagc gacaacggga tggcaagcag tgagatgagt gtggtcctga aactgccgag
    16861 aaaaacaatg agaatgattg taacaaatat cgccccctca atcagggcac gctgtacatt
    16921 ccttaccgac ctctcaatga agtctgcctg ccggaatatt tttgtatcca gccgcacatc
    16981 tggtggcaga ctgtttttca ggctttccag gttcttttct atctgcctgg ttacttcaag
    17041 ggtatttgca tttggctgct ttgatactga aattattaca gcaggttcgg cattgctaga
    17101 ggcatgcccc atctttacgg cactgccgat tctgacctca gcaatatcac ttatcctcac
    17161 aatattgccg gctgcagtct ttatataact gtcgcctata gcttccagat ccggggttct
    17221 ccctattccc cttatggcat attcattccc gtattgcctt aaaaccccgc cgctggaatt
    17281 ccgggccagt ccccttacag ccccggcaag ttcctgcatg gtcactctga aatatttcat
    17341 tttctgaggc tcagccagaa cctggtactg cttgtaatca cccccggtaa tggttacctg
    17401 tgaaacaccc ccggtggaaa gaataagagg ctttacactc cactcggcga gtgtccgcaa
    17461 atccatcaga ctggtactgt ctgactgtat gcctatgaaa aatatctcac ccatcacgct
    17521 tgactgcggt gcaagcatgg gctgtcccac tcccataggc attccggatg aaataccgat
    17581 aagcttttca ctcacaatct gacgcgccct gaaaatatcc gttccccagt caaactcaac
    17641 ccatacaaat gaatatccct gggaagaggc tgatctaacc cttctgacgt cagtagcacc
    17701 attcacggca gtctctattg gaaaagtaac catcctttca acctcttccg gcgccatacc
    17761 gtgggcatca gtcattacca caaccgtagg agcggtaagg tcgggaaata catcgatctc
    17821 catacgggat gcggttctgc ttccataaac cagcagcagt gccgaagcaa tcagaataag
    17881 aaacttgttg ttcagagaga attttatgat actgtccagc atggctgttc attttaatgg
    17941 acatggccgg aatgagcgtc aagcgcgccc gtggcctgtg caagtttgat atagatggct
    18001 ccctctgtaa ctattctctc atctgcccgg agaccttccg ccacctcggt acggtaacca
    18061 tccgtcctgc cggttctgat ctcccgtttt tcaaatagtt cgggagtgtg ctgaacccat
    18121 acaaaataga caccctgctc ttcaagtatg gcagtatttg gtacaaccag tgtatcttcc
    18181 ctgtccccgg cttcaagaaa cacctcaacg aaacttcccg ggataaactc accatgactc
    18241 tcaataccca gagtgacagg tatcaggaaa ttatcttccc cggcggcttt gccgtatgac
    18301 aggattctgc ctccaagttc atcgagactg taggtcctgc cggcattgat cttcctgatt
    18361 cttactgcct taatctggtg aagctgtgag gaatatcttt gcgggacatc cacttttaca
    18421 gccaggaaac tgttttgcaa tatgacgaca agagtctggc cggcatttac atattctcca
    18481 ttgctgacca ttatccgcat tataaatcct tttgcaggac tggtgacaag ctgtccggta
    18541 ttgttgaagc tttccctgta actctcatat accgatctgt cattttcaaa acggttttta
    18601 atctccagaa attccttttc cggtattatt ctgtccttca gaagagataa tgccctctcc
    18661 atgtcggcgg aggacctctc aaaattgttt ttcgcctgtg aatacctgac cgagaaatta
    18721 ttgtcagcca ggctgctgcc gctaaccgta aacaagatct gcccctgacc gacttcatcc
    18781 ccttcataaa taccacccgg gccaaactgc actattcccc ctgttctggc agacactata
    18841 aattcattac tgctcagcgg ctgaaccagt ccggttgttt taatcacctg ggcaaaagcc
    18901 tcctgccggg ggtatccggt ggaaaaatcc accttccacg actgttcctt tgtaaaaact
    18961 gaagtgtttg cctctgaatg agccccgtct tccagtgcct catggaccgc ctcatggttt
    19021 ccgtaaacct caacattatt gacaataagc tcacattccg attctccggc tgaaatatta
    19081 tatttaagaa tgccgcttcc cgcagattca ggaacaaagg agaaactgta gattcccttt
    19141 cctgaggaag ccgtattttc aggcacaaac tccttcccgt taataatata taccagtctt
    19201 atctttgcat ccccgacagc cctgaaaccc gggactgaac ttaaataggt gacaatactg
    19261 gcaggctcac cggcaacaaa atgatctgtt tctataaaaa gctcgaatct gtcgcagtaa
    19321 gccgtatact gaatcatgtg atcgtgatgt tcagaatgat gatcctctgg cttattacca
    19381 catcctgtaa gcaggatgac tgatatgaaa aaatataata atctgaattt cattttcctg
    19441 gcttaaattc atgaaatacg aaataacacg gcaggtatgt tccttgccgg tgtaaaaaaa
    19501 ttttatctca gatggaattt aagccgcagg aggagccctg agacctgaag caggatagga
    19561 atcctttaac tgatactttc cggatcctct ttcaaagacc aaaaagctgg gcctgttctg
    19621 atttatgtat aataaacctt ctcctgtcag tgaattgtaa tctgaattta acagttggtc
    19681 cggagtaata ttctcgaatg taccgatctg tattttttca tttatttccg gaaccgtcac
    19741 ctccagttca agaaaacaat tatcaccctg ctgacccttg tcattgccgt gagaaccggt
    19801 actttcactt tcattctgca ggtcatcaaa tgacagactg ccggagggca gacaagcaca
    19861 tggctgcctg tgtgccgttg cgaaacaagt aaaagggcca tggtggtgat gaggaataac
    19921 agtgcgggca aggaaggcca tggttcccag aattattatc aggggtatct ttaagacttt
    19981 attcctcata ttgttaaatc ctgtgcaaaa gtaacaaatt aataaaccag cttgttcaat
    20041 ttaacttctt ttaacctggc tgattcataa agcgaaaccc ggtatgccgg cagatccggg
    20101 ccgtacaggg ctgatcatca gcagctatcg gcagtcatca gccgtcatcg cccgggagat
    20161 taaacctccg ggactggctt gctgaaatcc cccggaggag ttatgtcagt ccccgtccgc
    20221 tttactgaaa caaataaaat gttaaaaacc ggaaaaataa gttgaaaaac atttttgttt
    20281 tttgtctctt cataaactaa atttgtttag aactactcga tcggaataca tggcattcaa
    20341 aataaaaaaa aattcgtccc ggttttgcct gttgttcacc cttatcacag ccatcccggt
    20401 tcacgcacag gatttatctg aactgaacaa gtcagagccc tttactatca gcgggagtgc
    20461 aggactgacc agttccttct attctgcctc cggcatcccg gatcgccagg caccctttgc
    20521 ctatggcatc catgccaatg ccaccatgac cctttatggc atagcaatgc ccttttcctt
    20581 tacctggtac aacaacagca aagtgggttt cagtcagcca ttcaaccagt tcggaatcag
    20641 tccgaaatac aggtggctca ctcttcacct gggctaccgc aatctgtcct tctctgaatt
    20701 caccctgagc ggttacactt ttctgggtgc cggtattgaa gccagtccgg gcaggttccg
    20761 catcggtgcg atgtatggta agtttaacca gaactccgtg tatgacctgg cgatggccga
    20821 ctcaatgccg aaaatgaccc ggacaggctg ggctgccaag ctgggctacg gcagcgagaa
    20881 acgatttgtc gacctgtcgg tgctgcggat aggtgacgat cccaaaggat atgacactct
    20941 taatatcgaa ccgggaatac cggcacccga acagaatatt gcactgggac tcacctcccg
    21001 ctttgacata agcgaaaaac tgaccttcag gtttgacggt tcatacagtt tctacacctt
    21061 caaccgcgcc tttgccgcca ccggcatcag cgatgacgcc ctgctccggc tggcaggcaa
    21121 tttcatcagg gtgaacagca ccagcgaata cttcaaggcg ctcaagacca gcatcagcta
    21181 ttccttcagc cagggaaccg taaccggtat cgagtaccgg cgtatcgacc cgggcttccg
    21241 gtccatggga agttatttct tcaacaacga tattgaacac ctgaccatca accagagcct
    21301 ggctctcctg aaaaacaaaa ttaacctgcg aggctcactg ggggctcagc gtgataacct
    21361 gaaaggggcc aaggagcata ccgcactcag gatcatcgga tccctcaccg gcaattacaa
    21421 cataaaccag aactgggcag tagatgcaac attcaataat ttctcaacca atcagcgtgt
    21481 attcaggaca gcaacagacc aaagcctgct catctaccag gtcaaccgca cgataacact
    21541 gtccccgcgc tacatgaacg ccggcgaaaa aatgtcgcac atggccatgc tgaacatcaa
    21601 tgttacagcg cttgaagaca aaaaccagag cacttcagac atgactgaaa ccgaaaccta
    21661 tatggcaatg ctgatctaca acctgggcct gcttcagctt aagatgaaca tttccgccgg
    21721 actcaactac accatgatgc tgaacaaaaa ctttgagaac aggctggcag gcggcaatat
    21781 caatgttgcc aaaacactgc tccgggacca gttatcactt ggactgaaca ataacctgat
    21841 gatcaacagg atcaacgatg acaagggtct gatcctcaac tcggcgctga cagctgcctg
    21901 gaggttccac ccgaaacatt cactctcact taaccttaac cttatcaaca accggtttgc
    21961 cgaaggatcg gcagtaccga catatcacga aataagagga gactttggct atgcgttcac
    22021 attctgacag atcacacgaa aaagaagaaa agacaatggc atctgccaca ttccgcaaag
    22081 accggttccc ggtacggtat ccggagacat cctgccggga gagttttaag acatcgggac
    22141 aggctgcagg atacccggca tcatcccggg ctgt
//
LOCUS       DAOSPX010000013        21895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2576_length_21895_cov_3.706090, whole genome shotgun sequence.
ACCESSION   DAOSPX010000013 DAOSPX010000000
VERSION     DAOSPX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21895
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_21895_cov_3.706090"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /gene="rimO"
                     /locus_tag="PLZ75_01390"
     CDS             <1..1267
                     /gene="rimO"
                     /locus_tag="PLZ75_01390"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOS71083.1"
                     /translation="LGCSKNVVDSEKLLARFRSAGYSPIWDPGDTTADTVIINTCGFI
                     NDAKEESIDTILRFVKAKESGSVRKLYVMGCLSQRYKAELQKEIPEVDGYFGVNNPEE
                     VLEITGGGFRDSLPDNRILTNPGHYAYLKVSEGCNRHCAFCAIPSIRGRYVSRPVEKL
                     EEEARRLADMGVREIILIAQDLGWYGLELYKKRKLPDLLHSLLRSENFSWIRLHYLYP
                     DYFPEEIISLMRDNPQICKYIDIPIQHISDKMLRLMKRSYGRREAEELLYRLREQLPG
                     AAFRTTLISGHPGETDSDHRELADFVRQFRFDRLGVFRYSHEEGTYSGDRYSDDITEK
                     LKEERVSELMAIQQSISLEKNYSLAGRTMKVLIDRKENEYYAGRTEFDSPEIDQEVLV
                     PVQYDLKPGNFYDIRITQTAEFDLFGDPA"
     gene            complement(1286..3844)
                     /locus_tag="PLZ75_01395"
     CDS             complement(1286..3844)
                     /locus_tag="PLZ75_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOS71084.1"
                     /translation="MDSQFSQRVKDILAYSKEEAIRLGNSHISPEHLFLGILRDGEGV
                     AVDILINRGVDLMGLKSSLEKSIKVESPVPVADHEIIPLLRSTERILKLVYLEAKSLK
                     SSSIESEHLLLAILKDENSLITRFLTEMDVDYQTVKKMIQTGPIRASADYPRDEDDEG
                     QGFGSSGKGSASASSAGKTSSDTPVLDNFGIDLTKAAEEGRLDPVVGREREIERLAQI
                     LSRRKKNNPVLIGEPGVGKSAIAEGLALRITKKNVSRVLFHKRVVALDLASIVAGTKY
                     RGQFEERMKAILNELSKNDNIILFIDEIHTIVGAGGATGSLDAANMLKPALARGEIQC
                     IGATTLDEYRQHIEKDGALERRFQKVLVDPTSEEETINILQNIKARYEDHHNVVYTDD
                     AIDACVKLTARYISDRHLPDKAIDALDESGSRVHISNIVVPERILSLEKQLDDTKNEK
                     MTAVKNQNFEKAASFRDREKDLLEMIDVEKKKWEKELSVNRETVDAEKVAEVVAMMTG
                     VPVQRIAQTEGTRLLRMADELKGSVIGQDEAIQKIVKSIQRNRAGLKDPNKPIGTFIF
                     LGPTGVGKTQLAKVLAKFLFDTTDNLVRIDMSEYMEKFSVSRLVGAPPGYVGYEEGGQ
                     LTEKVRRKPYSVVLLDEIEKAHPDVFHILLQVLDEGQLTDSLGRRVDFRNTIVILTSN
                     IGTRQIAEFGHGIGFDTSSRKASRSEQTKSILQKALQKTFAPEFLNRIDDIIMFNSLT
                     KEDINKIIDLELQGLYDRVRTLGYQLKIVPAARDFLAEKGYDATYGARPLKRAIQKYL
                     EDPMAELLIKAEAEEGDTIHVGFNSVKSEIRIRMRKKKETAEEQNKSSSEDEQN"
     gene            4106..6577
                     /gene="gyrA"
                     /locus_tag="PLZ75_01400"
     CDS             4106..6577
                     /gene="gyrA"
                     /locus_tag="PLZ75_01400"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOS71085.1"
                     /translation="MAERERVVRVNIEEEMKSAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLYGMSELGVLSNRPYKKSARIVGEVLGKFHPHGDSSVYDAMVRMAQEWSLRYPLV
                     EGQGNFGSVDGDSPAAMRYTEARLQKIAEETLADIDKDTVDFQPNFDDSLTEPVVLPT
                     RIPILLVNGSSGIAVGMATNMPPHNLAEIVDATIACIDNPEITNEELLEHIKGPDFPT
                     GGIIYGEQGIKDVAETGRGRIVIRAKTEIELTHSGRECIVVTEIPYMVNKAEMIRKIA
                     DLINDKKLEGISYINDESDRNGMRIVIILKKDATANVVLNNLFKFSQLQTTFNVNNIA
                     LVKGRPRTLTTRHLIDNFIQHRHEIIVRRATFDLAQAEKRAHILEGLIIASDNIDEVI
                     AIIKASENPDTARERLMERFDLSEVQSRAIVEMRLRQLTGLEQEKLRAEYNEVMALIE
                     YLRKVLGSVELQMNIIKDELAEIKQKYGDKRRTMIIPNAEEFNPEDFYADEEMVITIS
                     HMGYIKRTPLTEFRRQNRGGTGARGGTTRDEDFIEHLYIATMHNTMLFFTSNGKCYWL
                     KVYNIPEGSRISKGRAMQNLINIEPGDNVRAFINVKNLQEPDYINNNYIVLCTTKGII
                     KKTSLEAYSRPRANGVNAITVREGDELLEARMTNGDHHIMLAVRSGRAIRFPEDYVRP
                     MGRTASGVRGIRLENEETDYVVGMITVENKEKDILVVSEKGYGKRSDIDAYRITNRGG
                     KGVKTINITEKTGSLIALKSVNDNYDLMIITQFGNILRSPVSALRVMGRATQGVRLIN
                     LKENDTIASVACVEVNEENGKEFED"
     gene            6644..7816
                     /locus_tag="PLZ75_01405"
     CDS             6644..7816
                     /locus_tag="PLZ75_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS71086.1"
                     /translation="MKTFYSLLLLICISLGALAQKGKVTSALSFIEQGALDKAKDALE
                     QAYVHEKSKDWFNTYFAKGKFCQAVYQANDPKYNSYCTDPLGEAYAAYEKAISMDPKG
                     SVKKRIITNMIYNSLALDLYSQGSDRFEANDYAGALKSFATQIEITESDKYAGAVDTG
                     MYYNAGLAAINSKQYNDAIKYFQKCADMHYLGITPHIQVYESYMGLGDTIKAESYLLD
                     LPNKFPGDNQVILQLIDFYLKADKPDQAQKYITMAKEADPDNYSLYFAAGIMYLNQNN
                     FDEAIAELTRSIELKNDLYDTQYGLGAAYINKAADMFLKANDIMDVNEYTQAVEAANS
                     VYAKALPYMEKALELKPDDVYALRSLQELYYRLRQKDPSLNAKYEQTRARLSELEQ"
     gene            7925..8728
                     /locus_tag="PLZ75_01410"
     CDS             7925..8728
                     /locus_tag="PLZ75_01410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS71087.1"
                     /translation="MRKVNLILLIAFLIFSGCSRKEEILLPENPRIVVLMYHKIAHEE
                     AENLYERSRVEFEKDLNYLIKNNISVISFDDLENIKKSGKMPLTHSVIITFDDGDRSW
                     YDIAVPLLKKYGMKATFFLWVEKMEQHSFLSWQEIKYISNITLPGGVKPFVFGSHTYS
                     HPFLEGIKDHFETTEEYYQVLDWELARSKEIIEENTPGEVTVLALPYGDGAGNREIIA
                     SAMQSGYRFIRTSRNAAIHNISGLDLFNIPSLPMLDETEQDEIGYYLEH"
     gene            complement(8967..10604)
                     /locus_tag="PLZ75_01415"
     CDS             complement(8967..10604)
                     /locus_tag="PLZ75_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOS71088.1"
                     /translation="MSEKFSISPGGEKFPIPGPEEYKAEFERLQKMVASEREKGREIV
                     VVMGVGFVGAVMAAIVADSVDKVTGQPSKFVIGMQRPSVRSYWKIPLLNRGISPVKAE
                     DPDVDPMIARCVKEKKTLIATYTYDVLKLADVVVVDVQCDYIKNELSNVRNGQTDMAA
                     LEASLHEIAKRIPPEALVLIETTVAPGTTEQVAYPIMKKIFRKRGIKTDPLLAHSYER
                     VMPGRNYVASIRDFWRVCSGINETARQRVEKFLKEVLNTEEYPLTVMDRPIESETAKI
                     IENSYRATILAFLDEWSVFAERNGVDLIKVIKAIRVRPTHSNMIFPGPGIGGYCLPKD
                     GGLGVWSYKHILGFEDDIFKITPMAIDINDTRGLHVGQLTRDALRNMGQPIASSEILV
                     LGAAYREDVGDTRYSGSEIVIRKLAEMGAEIRVHDPYVEHWWEFENQDSYPATGQSKA
                     RFFRNQEKLKEIHVENDLNKALKDADAVILAVPHKAYLDLDPDTVVKTIGQPAAIIDC
                     FGILSDDKIRRYFQLGCEVKGLGRGHIQRIKDEIRRA"
     gene            10990..11655
                     /locus_tag="PLZ75_01420"
     CDS             10990..11655
                     /locus_tag="PLZ75_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOS71089.1"
                     /translation="MKSISKSTLEFLSDLKINNYRDWFQQNKARYEDARSDFESFVQA
                     LLDEIITFDPILKGLEARNCIYRIYRDIRFSADKSPYKSHMGAVIVRGGRNSTGRYAG
                     YYVHVEPGDESIIGGGAYMPPSPWLSAIRDKISEQGEELADIINDRGFAAVFGEIEGE
                     KLKSAPKGYPKDHPFIGLLRYKSFLASRTVTDREVVSADFFSLTVKAFKIMKPFNDFL
                     NEY"
     gene            11680..12684
                     /locus_tag="PLZ75_01425"
     CDS             11680..12684
                     /locus_tag="PLZ75_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS71090.1"
                     /translation="MNGLERTNRFLRGEDVDRPPFHPIIMRWAARYAGVKYRDFCTIP
                     AEKCRAMIRCAEGFDIDWVTVMSDPWAEASAFGIRVEYPEDALPVDTGGHLPDAAAAS
                     GLKPCDPLRNSRCRNRIREIEIYRKLTRGRFFIVGWVEGPVAEYVDLRKASEASVDFL
                     TEPDAVEKAMEVITENAMVFIDLQIAAGAHCIGIGDSFCSQIGPELYYRFAFERQKRL
                     VEFIHSRNALAKIHICGNTESILEGVIKTGADIIDIDHMVPSMKEFTRLLADHQVFSG
                     KSDPVTVIQNGDIQSITESVRSDFQDAGGRCIVSAGCEITPETSTENMKLFSNAAKLL
                     "
     gene            12951..13487
                     /locus_tag="PLZ75_01430"
     CDS             12951..13487
                     /locus_tag="PLZ75_01430"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HOS71091.1"
                     /translation="MKKLILIILMISVSSGFLTAQSGNLEVGKKVPEWTFPDASGKNF
                     TMNEWPGKVLQVNYVDTDESDLNDSFNDAINKAVDVDKRIARESFQGFGIVDTKSTWK
                     PKGLVRMIAGNKAKRYNTTILFDYDATLQKLWGLPKDSYSVVLVDKNRVCRAVYTGKI
                     PESENEKIIQLIIALTKE"
     gene            complement(13501..14325)
                     /locus_tag="PLZ75_01435"
     CDS             complement(13501..14325)
                     /locus_tag="PLZ75_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HOS71092.1"
                     /translation="MYFTSNIKFLRKRKGRTQEDVAAALNLKRSTLSGYENGIAQPGI
                     EILVSFSKYYNISVDTLLKTDISGLSESQLGELERGYDAYIRGSNLRVLTTSVGTDNI
                     DNIELVSEKAKAGYTTGYADPEFISELPHFRLPFLSDERKYRTFQLKGDSMFPIPDGS
                     WVTGEFVQDWREIISGKAYILFTIDDGIVFKIVENNLAAEGRLVLYSLNPIYEPYEIH
                     INEVREIWKFVNYISNELPDPVLPEKQLFQTMAAMKNDLQRLKAKFAADISDAEEY"
     gene            14424..15686
                     /gene="dinB"
                     /locus_tag="PLZ75_01440"
     CDS             14424..15686
                     /gene="dinB"
                     /locus_tag="PLZ75_01440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOS71093.1"
                     /translation="MNKSSGDRSILHLDLDTFFVSVERLRNSRLIGKPVIIGGLSDRG
                     VVSCCSYEARKSGVSSAMPMKLARNLCPEAIIIRGDMEAYSNYSNIVTEIIASKSPLY
                     EKLSIDEHYVDLSGMDRFYGSCKWAHELRQYIIRETGLPLSAGLSVNKTVSKIATGEA
                     KPNGEIEVVKEGVLPFLDPLPIARIPMLGRKTSHVLRSMGIDTIFTLRNIPAEMLEKL
                     MGKRGIEIWKKANGIDSSPVISYSEQKSISTEHTFERDTTDMALLRQTLAGMVEKTAY
                     DLRKQEKLTSCVTVKIRYSNFDTHTLQKRIPYTAFDHVLTGVAKELFARLYQRRMLIR
                     LIGVRLSHLVRGVQQLDMFDDTPEKISLYMAIDRIRNHFGQDAVRRAAGVMTRGEREE
                     KEARRCEYAADEQKMIRERLRGYMMYER"
     gene            15676..18624
                     /gene="dnaE"
                     /locus_tag="PLZ75_01445"
     CDS             15676..18624
                     /gene="dnaE"
                     /locus_tag="PLZ75_01445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOS71094.1"
                     /translation="MRDKAVYLNCHSYYSLRFGTMSVEQLVEKAAGTGVESMALTDIN
                     NSTAIPEFVQECINNNIRPLAGIEFRRDNELLYTGIARNNRGMKELNEFLSKHNFEGT
                     PLPFPAPHFSEAYIIYPVKKPPGRKLFDNERTGIRKDEAGRLVSLGPDEKKSMIIMQP
                     VTFNVPDDIFIHKSLRAIDNNILLSHLKPSQCAGENEFFHSPAHLKQEFINYPGIIHN
                     TEKLTADCSISFDFGNQKNKKIFSASRYDDKLLLEKLAWDGMVYRYGKNNDEAKKRIR
                     HELEIIDKLGFSAYFLITWDIIRYSMSRGFYHVGRGSGANSIVAYCLKITNVDPIDLN
                     LYFERFINPKRSSPPDFDIDYSWKERDEVTDYIFKRYGYHHTALLGTINTFRGKSIVR
                     ELGKIHGLPKEEIDELIDNPFSGRNGSEISDIIFATGRRIADFPNLRSIHAGGILISE
                     EPVSCYTALDMPPKGFPTTQWDMYTAEEIGFEKLDILSQRGLGHIRESVEIIRENRSE
                     DVDIHAIHKFRNDPKIRQYLRTGETKGCFYVESPSMRGLLQKLRCDDYPTLVAASSII
                     RPGVARSGMMREYIFRFHNPDKFNYLHPVMKEQLEETFGVMVYQEDVLKVCHHFAGLD
                     LADADTLRRAMSGKYRSKKEMQRIVDRFFSNCRKKGYEEDLIREVWRQIESFAGYSFS
                     KAHSASYAVESFQSLYLKAHFPLEFMVAVINNFGGFYRTWVYVHEAKRYGASIQLPCV
                     NKSNFKTTIYGSDIYLGFIHILGFEAGLAKTVEDLRRAEGDFTDLENFVTRVRPGLEQ
                     IILLIRAGAFRFTGKKKSTLLWEVHMLINRGSPEKTRSLFAPPLRKFVLPDLRQNLLD
                     DAYDEIELLGFPVSLTWFDMLETSFRGEVAARDMIRFKGREIRMVGHLVTIKYIKTVK
                     KEWMNFACFIDCEGNYFDTTHFPQSLKNYPFKGSGTYLILGRVAEEFGYASLEVEKMA
                     RLPVRPDKRY"
     gene            complement(18665..19339)
                     /locus_tag="PLZ75_01450"
     CDS             complement(18665..19339)
                     /locus_tag="PLZ75_01450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOS71095.1"
                     /translation="MFNNLLFISLAPVFIIAWYIYSRDTYEKEPLRMLIKALYIGALC
                     VIPVAIIEDVLMSLYQGSDEIRKAAYTAFLVAGFTEEVFKYSAFLLYIWKDRNFNEKF
                     DGIVYAVYISLGFAGIENLMYVFRGGYGVGLMRALTAVPAHALFGIMMGYYFGLAKFS
                     KRFRAAGIAAAFIVPFAFHGLYDFLLMSNTPLLLTAFLPVLVFYWISGFRKISKHLHA
                     SAFRPD"
     gene            complement(19527..21773)
                     /locus_tag="PLZ75_01455"
     CDS             complement(19527..21773)
                     /locus_tag="PLZ75_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOS71096.1"
                     /translation="MITFIPGLLAFISIFICKRKTGGGEVDSKKIKSAYRALLKAAEN
                     NISQEDIPRIRAALDLAIKTGEGSVALTGEPQVLHALSVARIIAGEVGLGPTSIITAL
                     LHDSWHKLDLNSPELEKQFGQKVTGILKGFSRISNLESMQSSFQAENFRKLLLSLADD
                     VRVILIKLVERLEYMRNLEKAPEKDRLPVASETYFLYAPLAHRLGFYNIKSEMEDLAM
                     KVLEPEQYSYIVSRLRQTTAARNRFIKEFSQPLQEKLQAMNFNFSIRSRTKSIHSIMM
                     KMKQQGVDFEEVYDLFAIRIILRSEPENEKSDCWRAYSVVTDLYQPNPSRLRDWLSVP
                     KSNGYESLHTTVIGPRGRWVEVQIRSERMDEIAEKGLAAHFRYKGVKAEGGLDVWLGK
                     MREILESPEKEDTAFFDQIRSGLYTDEVFVFTPKGDLRQLPAGATLLDFAFDIHTAVG
                     STCVGGKVNGKNVTLRYVLQNGDHVDVLRSKNQKPKQDWLSFVVTSKAKSKIKLALNE
                     EKARAAAEGKEILMRRLKNWKINYNDTVIQRLINHYNFKTAQDFYYLIAEEKIELLEI
                     KEILQKEETAAAVHTDPVVSLKEVKEDTDTLYSDFLVIEDKVEGLDYKLAKCCKPVFG
                     DPVFGFVTISEGIKIHRKGCPNAHNMMARYPYRVVAAQWTKTERLTSFITSVKITGLA
                     NTGIVNQISEIISHYNVNIKSFNYNSEEGMFEGNLGILVPNNDVLYSIIKKIQSLKGI
                     HRVIRQSD"
BASE COUNT         6298 a         4926 c         4959 g         5712 t
ORIGIN      
        1 tctcggttgc tcaaagaatg tggtggattc agaaaaactg ctggcccgct tccgctctgc
       61 cgggtattca ccgatctggg atcccgggga taccactgcc gacacggtaa taataaatac
      121 ctgcggattc ataaatgacg caaaggagga aagtatcgac accatactcc gctttgtaaa
      181 ggcaaaggaa tcaggatctg tacggaaact gtacgtaatg gggtgcctgt cccagagata
      241 caaggcggaa ctgcaaaagg agatacccga ggtggatggc tacttcggcg tgaacaatcc
      301 tgaggaagtg cttgaaataa ccggcggcgg cttcagggac agcctgcccg acaacaggat
      361 actcaccaat cccggacact atgcctacct gaaagtctcg gagggatgca acaggcactg
      421 tgcattctgt gctattccct ctatcagagg acggtatgta tctcgacctg tagagaaact
      481 tgaggaagaa gcccggagac ttgctgatat gggagtcagg gaaataatcc tgattgcaca
      541 ggatctgggc tggtacggac tggaacttta taaaaagagg aaactgcctg atctcttaca
      601 cagtctgctc agatcagaaa atttcagctg gataaggctg cattaccttt atcccgacta
      661 ttttccggag gaaatcattt ctttaatgag agacaacccg cagatatgca aatatataga
      721 tatccccata cagcatattt ccgataaaat gcttaggctg atgaaaaggt cttacggaag
      781 gagggaggca gaggagcttc tttaccgcct cagggaacag cttcccggtg ctgcttttcg
      841 gaccaccctg atttcaggcc atccggggga gactgattcc gaccaccggg aactggctga
      901 ttttgtccgg cagttccgct ttgaccgcct tggtgtgttc cgctattccc acgaggaagg
      961 aacatattcc ggtgaccgtt acagcgatga tatcaccgaa aagctgaagg aagagagagt
     1021 gtctgagctg atggccatac agcagtcaat atccctggaa aagaattaca gtctggctgg
     1081 caggacgatg aaggtactga tcgacaggaa ggagaatgag tactacgctg gcagaacgga
     1141 gtttgactcc ccggaaatag accaggaagt gctcgttccc gtacaatatg atctgaaacc
     1201 cggtaatttc tatgatatca ggataacaca gacggccgaa tttgatttat tcggtgatcc
     1261 ggcctgatta ttcctcggaa gattattagt tctgctcatc ctccgaagat gacttgttct
     1321 gctcttccgc agtttccttc tttttccgca ttctgatcct gatttctgat ttgaccgaat
     1381 tgaagccgac atggattgtg tcgccttctt ccgcctcggc ttttatcagc agttcagcca
     1441 tcggatcttc caggtatttc tggattgccc gcttaagggg acgtgcaccg tatgttgcat
     1501 cataaccctt ctcggcaagg aaatcccttg ctgcgggaac tatcttcagc tgatagccaa
     1561 gcgtcctgac cctgtcatac aagccctgga gttcaagatc gataatcttg ttgatatcct
     1621 ccttggtgag ggaattgaac attataatgt catcaatcct gttgagaaat tccggtgcga
     1681 aggtcttctg aagagccttc tgcagaatgc tctttgtctg ttcgctgcgc gatgccttcc
     1741 tggatgaggt gtcgaaacct attccatggc caaactccgc aatctgcctt gttcctatgt
     1801 ttgaggtgag gatcactatg gtattgcgga agtcgacacg ccttccgagg ctgtcagtca
     1861 gctgaccctc atcaagcacc tgaagcagga tatggaaaac atcgggatgt gccttttcta
     1921 tttcgtcgag caggacaact gaatagggct tccttctgac tttctcggta agctggcctc
     1981 cttcctcata tcccacgtag ccgggaggag ctcccacaag acgggaaacc gagaattttt
     2041 ccatgtattc gctcatgtca atcctcacga gattgtcggt ggtgtcaaaa aggaactttg
     2101 ccaggacttt tgcaagctgg gtttttccca ctccggtggg tcccaggaaa atgaatgtgc
     2161 ctatgggttt gttgggatcc ttcagtccgg cgcggttgcg ctgtatcgat ttgacaatct
     2221 tctggatggc ctcatcctga ccgataacgc ttcccttgag ttcatccgcc atcctcagca
     2281 ggcgggtgcc ttccgtctgg gcaatccgct gaacgggaac cccggtcatc atggcaacaa
     2341 cctccgcaac cttttcggca tccaccgttt cccggttgac agacagttcc ttttcccact
     2401 tcttcttctc tacatctatc atctcaagaa gatccttttc gcggtcacgg aaactggcag
     2461 ccttttcaaa attctggttc ttgacagctg tcattttttc attcttggta tcatccagct
     2521 gtttttcgag cgacaatatc ctctccggta caacaatatt ggatatatgt acacgggaac
     2581 ccgattcgtc aagtgcatca atggccttgt cgggcagatg cctgtcggat atatagcggg
     2641 ctgtgagctt tacgcaggca tcaatggcat catcagtgta aacaacatta tgatgatcct
     2701 cataccttgc ctttatattc tgaagaatat ttatagtctc ctcttcagaa gtcgggtcaa
     2761 cgagaacttt ctgaaagcgc ctctcaagag ccccgtcctt ttctatatgc tgccggtatt
     2821 catcaagcgt ggtggcgccg atacactgta tctcacccct tgcaagtgcc ggcttcagca
     2881 tatttgccgc atcaagtgag cctgttgcgc ctccggcgcc cactatggta tgaatctcat
     2941 cgatgaaaag aataatattg tcattcttcg acagttcatt cagaatggcc ttcatccttt
     3001 cctcaaactg tcccctgtat ttcgtgccgg caacaatgga agccagatca agtgcaacaa
     3061 ctcttttgtg gaaaagaaca cgcgatacat tctttttggt aatcctgagg gcaaggcctt
     3121 ccgcaatggc ggatttgccg acacccggct ctcctataag aaccggattg tttttctttc
     3181 tcctggaaag gatctgggca agcctttcaa tctctctttc ccttccaacc acgggatcaa
     3241 gacgcccttc ctcggcagcc tttgtaagat caataccgaa attgtcaagc accggagtgt
     3301 ccgaagaggt tttgccggct gaagaagcgg aggcagatcc cttgccggat gaaccgaaac
     3361 cctgaccttc atcatcctcg tcgcggggat aatcggcact ggccctgatt ggacctgtct
     3421 gaatcatctt ttttaccgtc tgatagtcca catccatttc tgtaagaaac ctggtaatca
     3481 gggagttttc atccttaaga attgcaagaa gaaggtgttc tgattctatt gagctgcttt
     3541 tcagcgattt ggcttcaaga taaacaagtt tcaatattct ttcggtgctt ctcagaagcg
     3601 gaataatttc atgatcagcg acgggtacag ggctttcaac tttaatgctt ttttcaaggg
     3661 agcttttcag acccataaga tcaacgcctc tgttaataag gatatcaaca gcaacccctt
     3721 cgccgtccct cagtatgccg agaaaaagat gctcagggct gatatggctg ttccccagcc
     3781 ttatggcctc ttccttgctg taagcaagga tatctttcac tctctgcgaa aattgtgaat
     3841 ccataataat aattgtcgtt aatccttcaa atatctgaca tcagccatta tggcagtatc
     3901 cataagacaa aaatacatat aaatgaaaga acaaagttaa attaatgaga tattatagta
     3961 ttgcaacaat tatgcctatt tatgaacagc tgatgttaaa aattcacaat aatccggggt
     4021 gcagaatagt taaattttga ctattttcgg gagtcaaaat cccgaagggg ttttaacttt
     4081 ataattaatt aattattagt atattatggc tgaaagagag agggtggtcc gggtgaatat
     4141 tgaagaagaa atgaaatcag catacattga ttattcaatg tcagtgatag tatcccgggc
     4201 gctgcctgac gtaagggatg gcctgaagcc tgttcaccgg cgcgtgcttt acgggatgtc
     4261 ggaacttggc gtcctttcaa acaggcccta taagaaatca gcgagaatag tgggggaggt
     4321 gcttggtaaa ttccatcctc atggcgacag ttcagtgtac gacgccatgg tcaggatggc
     4381 ccaggagtgg tctctcagat acccgctggt tgaaggacag ggaaacttcg gctcggttga
     4441 cggtgacagt ccggcagcaa tgaggtatac tgaagcaaga ctgcagaaga ttgctgagga
     4501 aactctggca gatattgaca aggatactgt tgatttccag cctaacttcg atgattccct
     4561 cactgaaccg gttgtgctgc caacccgcat acccatactg ctggtaaacg ggagctcggg
     4621 tatagcagtc ggcatggcaa ccaatatgcc gccgcacaac cttgctgaaa tagtcgatgc
     4681 cacaatagcc tgcatcgaca atccggaaat aactaacgag gagctgcttg aacacataaa
     4741 ggggcctgac ttcccgacag gaggcatcat ttacggcgaa cagggaataa aggatgttgc
     4801 cgaaaccggc cgcggcagga tagtaatcag ggcaaagacg gagatagagc ttacccattc
     4861 ggggcgggaa tgcatagttg ttaccgaaat cccctacatg gtcaacaagg ccgaaatgat
     4921 ccgcaagata gccgacctga taaatgacaa gaagcttgag ggtatatctt atatcaatga
     4981 tgaatccgac aggaacggaa tgcggattgt gatcatcctg aaaaaggatg ccactgccaa
     5041 tgtggtgctg aacaacctgt tcaagttctc ccagctgcag actaccttca atgtcaacaa
     5101 tatagccctg gtaaagggcc ggccccggac actgaccacc aggcatctga ttgacaattt
     5161 catacaacac cggcatgaga ttattgtcag aagggcaacg ttcgacctgg cccaggcgga
     5221 aaaaagagct catattcttg aaggactgat cattgcaagc gacaatatcg atgaggtgat
     5281 agcgataata aaggcatcag agaatcctga tacagccagg gaaagactga tggagcgctt
     5341 tgatctcagc gaagtgcagt cacgggcaat tgttgaaatg agattgcgcc agctcacagg
     5401 tcttgaacag gaaaaactga gggctgaata caatgaagta atggctctta ttgagtatct
     5461 gaggaaggtg ctggggagtg ttgaacttca gatgaatatc attaaggatg aacttgcaga
     5521 gataaagcag aaatacggcg acaagcgccg gacaatgata attcccaacg cggaggaatt
     5581 caatcctgaa gatttctatg cggatgaaga gatggttatc accatttcac acatgggata
     5641 tattaaaaga acccccctca ccgaattcag gaggcagaac aggggaggca ccggtgcaag
     5701 gggcggaacg acaagggatg aggatttcat agagcatctt tacattgcca ccatgcacaa
     5761 caccatgctt ttcttcacca gcaatggtaa atgctactgg ctcaaggtat acaacatccc
     5821 tgaaggatcc cggatctcaa agggcagggc catgcagaac cttatcaaca tcgagccggg
     5881 cgacaatgtg agggccttca tcaatgtaaa aaacctacag gaaccggatt atatcaacaa
     5941 taactacata gttctgtgca ccaccaaggg aattattaaa aaaacatccc tcgaagccta
     6001 ttcgagacca agggcaaacg gagtcaatgc cattacggtg agggaggggg atgagctcct
     6061 tgaagccagg atgaccaacg gggatcatca tatcatgctt gcagtgagat cgggccgggc
     6121 aatacgcttt ccagaagact atgtacggcc catgggccgt accgcttccg gtgtcagggg
     6181 aataagactc gaaaatgagg aaacggacta tgttgtgggc atgattaccg tcgagaataa
     6241 ggaaaaggat atccttgttg tatcggaaaa gggatatggc aaaagatccg atatcgatgc
     6301 ctaccgtatc accaaccggg gcgggaaggg ggtaaaaacc attaacatca ctgaaaaaac
     6361 aggttcgctg atagcactga aaagtgtgaa tgataattat gacctgatga taatcaccca
     6421 gtttggcaat atcctcagaa gcccggtgtc tgcactgaga gtgatgggaa gggcaacaca
     6481 gggcgtgagg ctgatcaatc tgaaggagaa tgacaccata gcatcagtgg cctgtgtgga
     6541 ggtgaatgaa gaaaatggca aagaatttga agattaagaa gtaatattat aaatttggaa
     6601 aattatttaa gaacaaaatc tatttaaaac aaaagctata atcatgaaaa cgttctattc
     6661 actattgctg ctgatatgca tctcgttagg agccctggca caaaaaggaa aggtgacctc
     6721 tgctcttagc ttcatcgaac agggggccct ggataaagca aaagacgccc tggaacaggc
     6781 atatgtacat gaaaaatcca aagactggtt taatacatat tttgccaagg ggaagttctg
     6841 ccaggcagtt tatcaggcaa atgatccgaa gtacaattca tattgcaccg atccgctggg
     6901 agaggcctac gctgcctacg aaaaagctat ttcaatggat ccgaaaggaa gtgtaaaaaa
     6961 gaggatcatt accaatatga tctataactc ccttgccctt gatctttatt cacagggaag
     7021 cgacagattt gaagcaaacg actatgcagg agcactgaaa tcatttgcca cgcagataga
     7081 aataaccgaa agcgacaaat atgccggagc cgtggatacc ggaatgtatt acaacgccgg
     7141 acttgcagca ataaattcaa agcaatataa tgacgcaata aaatatttcc agaaatgtgc
     7201 cgacatgcat tacctgggca tcactcccca tattcaggtt tatgaaagct acatgggcct
     7261 gggtgatact ataaaggcag aaagttatct gctggatctg ccgaacaagt tccccggcga
     7321 caaccaggtt attcttcagc tgattgattt ctaccttaag gctgacaagc ccgatcaggc
     7381 acagaaatac attaccatgg caaaggaagc agatccggat aactacagcc tttattttgc
     7441 agcaggtata atgtatctga atcagaataa ctttgacgag gcaatagcag aacttaccag
     7501 gtcaatagag cttaagaacg atctttatga tactcagtac ggactgggcg cggcatacat
     7561 caataaggct gccgacatgt tccttaaggc aaacgatata atggatgtga acgaatatac
     7621 ccaggcagtt gaggcagcaa actcggtata cgcaaaagct ttgccttaca tggaaaaggc
     7681 acttgagctg aaaccggatg atgtttacgc cctcagaagc cttcaggagc tgtattacag
     7741 gctgagacag aaagatccgt cactgaacgc caaatatgag caaacaaggg ccagactcag
     7801 tgaacttgag cagtaaaagt gaaaacaaac ctgtgtcaga aatggacagg taaataaata
     7861 aacgaaaagc tttccatggg ggaaagcttt tctgatttta acaagtatca ataaaaacca
     7921 aagcatgaga aaagttaatc tgatattgtt aattgctttc ctgatatttt caggatgctc
     7981 acggaaagag gaaatcctgc ttccggaaaa tccccgtatt gtggtattaa tgtatcacaa
     8041 aatagcccac gaagaagccg aaaatcttta tgaaaggagc agggtcgaat ttgaaaaaga
     8101 ccttaattat ctgataaaga ataatatatc tgtaataagt ttcgatgatc tggaaaatat
     8161 caaaaagtcg ggcaagatgc ctttaacaca ttcagtcata ataactttcg acgatggcga
     8221 ccgttcctgg tatgatattg ccgttccgct cctgaagaaa tacggcatga aggcaacttt
     8281 tttcctctgg gtggaaaaaa tggaacagca ttcatttctt agctggcagg aaatcaaata
     8341 tatcagcaac attacgctgc caggcggagt taaacccttt gttttcggct cgcacactta
     8401 ttcacatccc ttcctggagg ggataaaaga ccattttgag actacagagg agtattacca
     8461 ggtccttgac tgggaacttg cccggtcgaa ggaaatcatt gaggaaaaca ctcccggaga
     8521 ggttacggta ctggcacttc cgtatggtga cggtgcaggt aaccgcgaaa ttatcgcatc
     8581 agcaatgcaa agcggataca ggttcatcag gacatccagg aatgccgcaa tccacaacat
     8641 ttccggcctt gacctcttca atatacccag tctgcccatg ctggacgaga cagagcagga
     8701 tgagatagga tattacctgg agcattaagc attgcaatcc tgaaattctg gatccttgtt
     8761 gcgattaatc tgcgacgaat actgattaaa aatctttact tttttaaaca tcattataaa
     8821 gaaatagata gaaggaagag ggcatatgtt tgttttctat ttaacataat attaattata
     8881 ggaaaacatg cattaaaaag aaatgagccg gaatggctca tttctgcaaa ctgaataaaa
     8941 agcttgagga aatgcagcag ctttagctat gcccgacgga tctcgtcctt tattctttga
     9001 atatgtccgc gtccgagtcc cttcacttca cagcccagct ggaaatatct ccggatctta
     9061 tcatcggata aaataccgaa gcagtctatt atggcagccg gctggccgat tgttttaaca
     9121 accgtgtcgg gatcaagatc gagataagcc ttatgcggaa ctgcaagtat cacggcatca
     9181 gcatctttaa gggctttatt cagatcgttt tcaacgtgta tctcctttag tttctcctga
     9241 ttgcggaaaa aacgtgcttt actttggcct gtcgcgggat aggaatcctg gttttcaaat
     9301 tcccaccaat gttcaacata gggatcatgt accctgattt ctgcacccat ttctgcgagc
     9361 ttcctgatca caatctcaga tccgctgtat cttgtatctc ccacatcttc acgatatgca
     9421 gctccaagaa ccagaatctc tgaactggct ataggctgac ccatatttct cagggcatcc
     9481 ctggtgagct gtccgacatg aagtccgcgt gtatcgttga tatcaatggc cattggtgtg
     9541 attttgaaaa tatcatcctc aaaaccaagt atatgtttgt atgaccagac tcccaggccg
     9601 ccgtccttcg gcagacagta gccccctatt cccggtccgg gaaatatcat gttactgtgt
     9661 gtcggacgca ccctgatcgc cttgatgact tttataagat ccactccgtt gcgttcggca
     9721 aaaacgctcc attcatcaag aaaagccaga atggtggccc tgtaactgtt ttcaataatt
     9781 ttcgctgttt ccgactcaat tggcctgtcc attacggtga gaggatattc ttcggtattc
     9841 aggacttcct tcaggaattt ctcaactctc tgtcttgcag tctcattgat cccgctgcat
     9901 actctccaga aatcgcgtat gctggccaca taattgcggc ccggcatcac tctttcatag
     9961 ctgtgtgcaa gcagaggatc ggttttaatc ccccgctttc tgaagatttt cttcatgatg
    10021 ggataagcca cctgttcagt cgtgccgggt gcaaccgttg tctcaatgag aacaagcgct
    10081 tccgggggaa tcctctttgc aatttcatgg agcgaggctt caagtgcagc catgtccgtc
    10141 tgtccgttcc ttacattact cagctcgttt ttaatgtaat cacactgaac atccaccacg
    10201 accacatcgg caagtttcaa tacatcatag gtatatgtgg caataagggt cttcttttct
    10261 ttaacacacc tggctatcat gggatcaaca tccggatcct cagccttaac cggggagatt
    10321 ccgcggttca gaaggggtat cttccagtaa ctgcgcacac tgggacgctg catgcctata
    10381 acgaactttg acggctggcc tgtcactttg tcaacactgt cagcaacaat agcagccatt
    10441 acggcaccca caaagcccac tcccatcaca acaacaatct cacggccctt ctctctttca
    10501 ctggcaacca ttttctgaag gcgttcaaac tcagctttgt attcctctgg accaggtatc
    10561 ggaaactttt cacctcccgg actgatcgaa aatttttcac tcatgttttt attgtttgat
    10621 taattaatgt gttttattcg gctgctaaag ataggaatgt ttaaataatc tgatctttat
    10681 gaagtttgat ttttgcaagc acgagtaata tgaacctatc aaccaggatt ttacagaatc
    10741 aaaattatta ttttgcagca taagggaaaa aattacgctt gttatacgta tattttgcat
    10801 actagttacc tgttaaacca atattttgcc ggaaaataaa tccgggtccc tgttacctga
    10861 ccggaaaccg ggaataaaca gcacgaaaca tattatataa agtagcatat tatatatacg
    10921 agtgccggac attccggact atttttttac ttttattcat taatcttgga cggcagacat
    10981 tattttttca tgaaaagcat cagcaaatcc actcttgaat ttctttctga tctgaaaatc
    11041 aataattacc gcgactggtt ccaacaaaac aaagccagat acgaagatgc cagatcagat
    11101 tttgaatcct tcgttcaggc attactggat gaaatcataa catttgatcc catccttaaa
    11161 ggactggagg ccagaaactg tatttacagg atttaccgcg acatcaggtt ctccgccgac
    11221 aagtcgccat acaaatccca catgggggct gttatagtac gcggaggcag gaacagcacc
    11281 ggaagatatg caggttacta tgtgcatgtg gaaccgggag atgaaagcat tataggcgga
    11341 ggtgcttaca tgccgccgtc tccatggctg tcagcaatca gggataaaat cagcgaacag
    11401 ggcgaagagc tggctgatat cattaatgac cggggattcg cagcggtatt tggtgaaatt
    11461 gaaggagaaa aactgaaatc agccccgaag gggtatccga aagatcatcc ctttatcgga
    11521 ctgctcaggt ataaaagttt tctggcctcc agaactgtta ctgacaggga agtggtcagc
    11581 gctgattttt tcagcctcac tgttaaagca tttaagataa tgaagccttt taacgatttc
    11641 ctgaacgaat actgattatt cacaaccatt ttgaaaacaa tgaacggtct ggaaagaacc
    11701 aatagatttc tcagagggga ggatgtggac cgccccccct tccaccctat catcatgaga
    11761 tgggctgccc gctatgccgg cgtaaaatac cgcgacttct gtacgatacc tgctgaaaaa
    11821 tgcagggcaa tgatcagatg tgcagaaggt tttgatattg actgggttac ggtaatgtcc
    11881 gatccctggg ctgaagcttc cgcctttggc atccgtgtcg aatatcctga ggatgctttg
    11941 ccggttgata ccggtggaca tttacccgat gcggcggcag catccggact gaaaccctgt
    12001 gatccgctcc ggaacagccg ctgccgcaac aggataaggg aaattgaaat atacagaaaa
    12061 ctgacaaggg gtagattttt tatcgtggga tgggttgaag gtcccgttgc ggaatatgtc
    12121 gacctcagga aagcatcaga agcctcggtg gattttctca ctgagccgga tgccgtggaa
    12181 aaggcaatgg aggtgattac tgaaaatgcc atggtattta ttgatctcca gatcgctgcc
    12241 ggtgcacact gtatcggaat tggcgattcc ttctgctccc agattggtcc ggaactgtac
    12301 tacaggtttg catttgaaag acaaaaaaga ctggtggaat tcattcattc cagaaatgcc
    12361 ctggccaaaa tccatatctg cggaaatacc gaatcaatcc tggaaggcgt gataaaaacg
    12421 ggtgcagaca taattgatat tgatcacatg gttccctcca tgaaagaatt tacccgctta
    12481 ctggcagatc accaggtatt ctcaggcaaa agcgacccgg taacagtgat tcagaacgga
    12541 gacatccaat ccattactga atccgtcagg tcagattttc aggacgccgg aggccgctgc
    12601 atagtatcgg ccggctgcga gattacacct gaaacgtcaa cagaaaacat gaaacttttc
    12661 agcaatgcag caaaactgct ctgatatgcc gggctgtaga attcaactga gtttccgcct
    12721 tcaatgtaaa aaaatcagca gctcatcaaa ctaatgaaat gctgaatgca ggattttact
    12781 gaaaaatact atatttcgtg aaatttacct gacggaatac aaaaactgca ttccgttaaa
    12841 acgcggcaaa aacacttaaa ccataacgcc accataaact aaaatatgtt cactattcat
    12901 tgcatagttt atttatcttg tattcaataa cttaataact cttatcccag atgaaaaaac
    12961 ttattctgat tatccttatg atttccgttt cctcaggttt tctgacagcc cagtccggta
    13021 atcttgaagt gggcaagaaa gttccggaat ggacatttcc tgatgccagc ggaaaaaatt
    13081 tcacaatgaa tgaatggccc gggaaagtat tgcaggtcaa ttatgtcgat acggatgaat
    13141 ccgatttgaa cgactctttc aatgatgcga taaataaggc agttgatgtt gacaagcgta
    13201 ttgccaggga gagtttccag ggctttggta tcgtggatac aaaatcaaca tggaaaccca
    13261 agggactcgt gaggatgatt gcgggcaaca aggcaaagag atataataca accatactgt
    13321 tcgactacga cgcgacactg cagaaactat ggggtctgcc aaaggacagc tatagtgtcg
    13381 tgcttgttga caagaacagg gtttgcagag cagtttacac gggcaagata cccgaatcgg
    13441 agaatgaaaa aatcattcag ctgatcatag cccttacaaa agagtaagag acaggttctg
    13501 ttaatactcc tcagcatcag atatgtcggc tgcgaatttt gccttcagcc tctgaaggtc
    13561 attcttcatg gctgccattg tctgaaacag ctgcttttcc ggaagcacgg gatccggaag
    13621 ttcattgctg atataattga cgaatttcca tatttccctg acttcattta tatgaatctc
    13681 atagggttca taaatgggat tcagtgagta caaaaccagc ctgccttctg cagcaaggtt
    13741 gttctcaact atcttaaaga ctatcccgtc gtcgatggtg aaaagaatat aggccttgcc
    13801 gcttattatc tcgcgccagt cctggacaaa ctcccctgtc acccacgaac cgtcaggtat
    13861 cgggaacatt gaatccccct ttagctgaaa agtacggtat tttctctcgt ctgacaggaa
    13921 gggaagtctg aaatgcggca gctcgctaat gaattcagga tcagcatagc ccgtggtata
    13981 gccggctttt gccttttcag aaaccagttc gatattatca atattgtcgg tcccgactga
    14041 tgttgtcaga actctcagat tgcttcccct gatatatgca tcatagcctc tttccagttc
    14101 tccgagctga ctttccgaca atccggaaat atctgttttc agaagggtat caaccgatat
    14161 attgtaatat ttcgaaaatg ataccaggat ctcaattccg ggctgggcaa taccgttctc
    14221 gtatccgctc agcgtggagc gcttaagatt caaagctgca gcaacatctt cctgtgtcct
    14281 gccctttctt tttcttaaaa atttaatatt ggaagtaaaa tacatatgaa aaatgattat
    14341 attgttatcg gtatattgat taatttataa tcaaaaatag aatataatta ttgtataaac
    14401 aaattttttc cggaaaatct gaaatgaata aaagcagcgg cgaccgttcc attcttcatc
    14461 ttgatctcga tacttttttt gtttcggttg agagactcag gaacagcagg ctcattggca
    14521 agcctgtgat aatcggaggc ctgtcggatc gtggtgtggt atcgtgctgc agttacgagg
    14581 caaggaaatc cggagtaagc tcggcaatgc cgatgaaact cgccagaaat ctgtgtcccg
    14641 aagcaataat aatccggggc gatatggaag cctacagcaa ttactcaaat attgttactg
    14701 aaataattgc atcaaaatca ccattatatg aaaagttatc tatagatgaa cattatgtgg
    14761 atctgagcgg gatggatcgt ttctacggaa gctgcaaatg ggctcatgaa ctgcggcagt
    14821 atataatcag ggaaacgggt ctgccgcttt cggcggggct ttctgtaaac aaaaccgtat
    14881 caaagattgc aacgggggaa gcaaagccaa acggggaaat tgaagttgta aaggaagggg
    14941 ttcttccttt cctggatcct cttcccatag caagaatccc gatgttgggc cggaaaacca
    15001 gccatgttct aaggtcaatg ggcatagata ccatatttac tctcaggaat atccctgctg
    15061 aaatgcttga aaagctgatg ggaaaaaggg gaattgagat ctggaaaaaa gcaaacggga
    15121 tagactcaag tccggtaatc agctattcgg agcagaaatc aataagcacg gaacatacat
    15181 tcgaaaggga taccaccgat atggcactgc tccggcagac acttgccggc atggttgaaa
    15241 agactgccta tgacctccgc aagcaggaaa agctcacttc ctgcgtgacc gtgaaaataa
    15301 gatattccaa ttttgatacc catacacttc agaaacgaat accttatacg gcttttgacc
    15361 atgttctgac cggtgttgca aaggagttat ttgcacggct ctaccagagg cgaatgctga
    15421 taaggctcat tggtgtcagg ttaagtcatc tggtacgggg tgtccagcaa ctcgacatgt
    15481 tcgatgacac acccgaaaag ataagccttt atatggcaat agacaggatc aggaaccatt
    15541 tcgggcagga tgcggtgcga cgggctgcag gcgtgatgac ccgcggtgaa agggaggaaa
    15601 aagaagccag acggtgtgaa tatgccgcag atgaacagaa aatgatcagg gaaagactga
    15661 gaggatatat gatgtatgag agataaagcc gtgtatctca attgtcattc atattacagc
    15721 cttcgcttcg ggacaatgtc cgtggaacag ctggtggaaa aagctgccgg cacaggtgtt
    15781 gaatcaatgg cactgaccga cataaataat tccaccgcga ttccggagtt tgtacaggaa
    15841 tgcattaaca acaatatccg cccgcttgcg gggatagaat tccggaggga caatgaactg
    15901 ctttacacag ggatagcccg gaacaacagg ggaatgaagg aacttaacga attcctgtca
    15961 aagcacaatt ttgaaggaac ccctctccca ttccctgcac cgcatttttc agaagcatat
    16021 ataatatacc ccgtaaaaaa gcctccgggc cgtaaattat ttgataatga gagaaccgga
    16081 ataagaaagg atgaagcggg ccggcttgtc agcctgggcc ctgatgaaaa gaaaagcatg
    16141 atcataatgc agcccgtcac tttcaacgta ccggacgata tttttataca taagagtctg
    16201 agagccattg ataacaatat tcttctcagt catctgaagc cttcacagtg tgccggggaa
    16261 aatgaattct tccattcccc tgctcatctg aaacaagagt tcataaatta tcccggaata
    16321 atacataaca cggaaaagct gaccgctgac tgcagcatat ccttcgattt cgggaaccag
    16381 aaaaacaaaa agatcttctc cgccagccgc tatgacgata aactgcttct tgaaaaactg
    16441 gcatgggacg ggatggtcta ccgctacggc aaaaacaatg acgaagcaaa aaagagaatc
    16501 cggcatgaac ttgaaataat cgacaagctc ggcttctctg catacttcct gataacatgg
    16561 gatattatca ggtactcaat gtccaggggc ttttaccatg tgggcagagg aagcggagca
    16621 aattcaattg ttgcttattg tttgaaaatc accaatgttg atccaatcga tctaaatctt
    16681 tacttcgaac gatttatcaa tccgaaaaga agcagtccgc ccgacttcga catagattat
    16741 tcctggaagg aaagagacga ggtaacagat tatatattca aaagatacgg gtatcatcac
    16801 actgccctcc tgggaacgat caataccttc aggggaaaat ccattgtgag ggagctcgga
    16861 aagattcacg gattgccgaa agaggaaatt gatgaactga ttgacaatcc cttttccggg
    16921 agaaacggca gtgaaataag tgatataatc tttgcaacgg gacgcaggat tgccgatttc
    16981 ccgaatttaa gaagcattca tgccgggggc attcttattt ccgaagaacc tgtttcatgc
    17041 tatactgccc ttgatatgcc gcccaaagga tttccgacca cccagtggga tatgtacact
    17101 gccgaggaga taggtttcga aaagctggat atactcagcc agagaggcct gggacatatc
    17161 agggagagcg tggaaatcat acgggaaaac cgttcggaag atgtcgacat acatgcaatc
    17221 cataaattca ggaacgaccc taagatcaga caatatctcc gtaccgggga gaccaaagga
    17281 tgcttctatg tggaaagccc gtcaatgcgg ggattgttgc agaaactcag atgcgacgat
    17341 tatccgacac ttgttgctgc cagttcaata atcagacccg gggtggcacg ttcgggaatg
    17401 atgcgggaat atattttccg ttttcataat cctgataaat tcaattactt acacccggta
    17461 atgaaagaac agcttgagga gacatttggg gtaatggtgt accaggaaga tgtcctcaag
    17521 gtgtgtcatc attttgcagg cctggacctt gcagatgcag atacgcttcg cagggcaatg
    17581 agcggaaaat accgttcaaa aaaggaaatg cagcgcattg ttgacagatt tttctccaac
    17641 tgccgtaaaa agggctacga agaggatctt atcagggagg tatggagaca gatagagtca
    17701 tttgcaggct attcgttttc aaaagcccat tcggcatcct atgccgttga aagttttcag
    17761 agtctttacc ttaaggcaca tttccccctg gaattcatgg tggctgttat caataatttt
    17821 ggcggattct acaggacctg ggtctatgta catgaagcaa aacgatatgg tgcctccatt
    17881 cagctgccat gcgtcaacaa aagcaatttc aaaacaacta tctatggttc tgatatctac
    17941 cttggtttta tccatattct gggtttcgag gccggactgg caaaaacagt ggaagatcta
    18001 cgccgcgcag aaggcgattt caccgatctt gaaaatttcg tgacgagggt caggcccgga
    18061 cttgaacaaa taatacttct cataagggcc ggggctttcc gctttaccgg aaagaaaaaa
    18121 tccacactgc tgtgggaggt ccatatgctg ataaacaggg gaagccctga aaaaaccagg
    18181 tcactctttg ctcctcccct caggaaattc gtcctccccg acctcaggca gaatctgctc
    18241 gatgatgcct atgatgaaat agaactgctc ggcttcccgg tatctctgac atggttcgac
    18301 atgctggaaa catcattccg gggagaggtt gcggcacgtg acatgatacg cttcaaaggc
    18361 cgggaaatca ggatggtcgg acatcttgta actataaagt atattaagac agtgaaaaag
    18421 gaatggatga attttgcctg tttcattgac tgtgaaggaa attacttcga caccacccat
    18481 ttcccgcaat cacttaagaa ctatcctttt aagggatcag gtacttatct catcctgggc
    18541 agggtggcag aagaattcgg atatgcctcc cttgaagttg aaaaaatggc caggctcccg
    18601 gtcagacccg ataaaagata ctgacaagct tacggatata tcaaaagggc cgtttctttc
    18661 cgtctcaatc cggcctgaag gcagaggcat gcaaatgttt cgatattttc ctgaagccgc
    18721 tgatccagta aaaaacaagg acgggaagaa aagccgtaag cagcagcggg gtattgctca
    18781 tcagaaggaa atcatacaat ccgtggaagg cgaagggaac aataaaagcc gctgcaatac
    18841 cggctgccct gaaccgtttg ctgaatttcg caagtccgaa ataatatccc atcattattc
    18901 cgaaaagagc atgggccgga acggctgtaa gtgcacgcat cagaccaacg ccataccctc
    18961 cgcggaaaac atacatcaga ttctctatcc ctgcaaatcc gagggaaata taaacagcat
    19021 acacaatccc gtcaaacttt tcgttgaaat tcctgtcctt ccatatgtaa agtaaaaatg
    19081 ctgaatattt aaaaacctcc tcggtaaatc ctgctaccag aaaagcagta tatgccgcct
    19141 tccttatctc atcagagccc tggtacagac tcatcagaac atcctcaata atagctacag
    19201 gtataacaca taaagcacct atatataaag cttttatcaa catccttaaa ggctcttttt
    19261 catatgtgtc gcggctgtat atgtaccagg caattataaa gacaggagcc agtgaaataa
    19321 ataacaaatt attaaacata ataccggaag ttcagtcagg taagctttta atcaaatcaa
    19381 acttttataa aactggtttt aaaaatgtga attaataaaa aaaaacagat tctaaaatta
    19441 agcaagtctt tttgttaaca agtcatataa catacaatac aaggtgaacc acaggatcag
    19501 cttaaggact gatcgttgct gtttttctaa tcactctggc gtattaccct gtgtattcct
    19561 tttagtgact ggattttttt gattatgctg tacagtacat cgttgttggg aactaaaata
    19621 cccaggtttc cctcaaacat tccttcctcc gagttgtaat tgaaactctt tatgttaaca
    19681 ttatagtgtg aaattatttc ggatatctgg tttactatac ctgtatttgc gagtcctgtt
    19741 attttgaccg aagtgatgaa ggatgtgagg cgttccgttt tggtccactg tgcggcaaca
    19801 accctgtaag gataccgtgc catcatatta tgtgcattcg gacatccctt tctgtgtatt
    19861 tttatacctt ccgatatggt aacaaatccg aacacgggat ccccgaatac cggcttgcag
    19921 cattttgcca gcttgtagtc gagaccttcc actttatctt ctatcaccag gaaatcggaa
    19981 tacagagtgt ctgtatcttc ttttacctct ttcaggctta caacgggatc agtgtgaact
    20041 gccgcagccg tttcctcctt ctgaaggatt tccttgatct caagcagttc tattttttct
    20101 tccgctatca ggtaatagaa atcctgggct gttttgaaat tataatgatt gataagcctc
    20161 tggatcacag tatcattata atttattttc cagttcttca gacgccgcat caggatctcc
    20221 tttccttctg cggcagccct ggctttttcc tcattgagtg caagcttgat tttcgatttt
    20281 gccttgcttg tcaccacaaa cgacagccag tcctgcttgg gtttctgatt ttttgaccgc
    20341 agaacatcaa catgatcccc gttttgcaga acatatctga gagtgacgtt ttttccgtta
    20401 accttgccgc cgacgcatgt ggaacccacc gctgtatgaa tgtcgaaagc aaaatcaaga
    20461 agggttgcac cggcaggcag ctgtctcaga tcaccctttg gagtaaaaac aaatacttca
    20521 tcggtataaa gtccggaacg gatctggtca aaaaaagcag tatcctcttt ttcaggagat
    20581 tcaagtattt cccgcatttt acccagccac acatcaagtc cgccttctgc ctttacccct
    20641 ttgtagcgga aatgcgctgc aaggcccttt tctgcaattt catccattct ttccgacctt
    20701 atctgcacct ccacccacct tcctctcggt cctatcacag ttgtatggag ggattcatag
    20761 ccatttgatt tgggaacaga cagccagtca cgcaaacgac tcgggttcgg ctgataaaga
    20821 tctgtcacca ctgaataggc gcgccagcaa tccgattttt cattttcggg ctcgctgcgc
    20881 agaattattc ttatggcaaa cagatcatat acttcctcaa aatcaacccc ctgctgtttc
    20941 attttcatca tgatggaatg aatcgatttg gtccggcttc tgatgctgaa gttaaaattc
    21001 atggcctgaa gcttttcctg caggggctgc gagaattcct ttatgaacct gttacgggcg
    21061 gcagtcgtct gcctgaggcg gctgacaata tagctgtact gttccggttc aaggactttc
    21121 atggccagat cctccatctc cgatttgata ttatagaagc caagcctgtg tgcaagaggc
    21181 gcataaagaa aataggtctc ggaggcaacg ggaagtctgt ctttttcagg agctttttcc
    21241 agattgcgca tatactccag cctttcaacc agcttgataa gtatgacacg gacatcatcc
    21301 gccaggctta acagcagttt cctgaaattt tccgcctgaa aagatgactg catggattct
    21361 aggttgctga ttcttgaaaa acctttcaga atcccggtca ctttctggcc aaactgcttt
    21421 tccagttcag gagagttaag atcaagttta tgccaggaat catgaaggag ggcggtaatt
    21481 atagatgttg gccccagtcc cacttcaccg gcaattatcc ttgcaacaga cagtgcatga
    21541 agtacctgtg gttctccggt aagggccaca gacccctcac ctgtttttat tgccaggtca
    21601 agtgcagccc tgatccttgg gatatcttcc tgtgaaatgt tattttccgc tgctttaaga
    21661 agtgccctgt atgctgattt tatctttttt gaatccacct ctcccccgcc ggttttcctt
    21721 ttgcaaataa atattgaaat aaaagccagt aatcccggaa tgaatgttat catgtccggg
    21781 attatagttt atggttaaca gcctgtgtac cagtatcaga aacagtcccg cctaactgta
    21841 aaatgaaatg cggttaacat ggcagtactg accggcatac gatatgctga tacag
//
LOCUS       DAOSPX010000014        21688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2614_length_21688_cov_4.375815, whole genome shotgun sequence.
ACCESSION   DAOSPX010000014 DAOSPX010000000
VERSION     DAOSPX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21688
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2614_length_21688_cov_4.375815"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLZ75_01460"
     CDS             complement(<1..863)
                     /locus_tag="PLZ75_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71097.1"
                     /translation="MSRLLVITTLILLIVLPGELHAQLTSPFSVDPSKYREQLISFMG
                     PNLKENHKEELNLFLQNWESQKFDTEDMTRIIDVSSQFYGRSMRPVPHMYNFLVTLNT
                     YIRAGNDREFLSNWLKGLSEIVFDPRISVDKINRYIRDTELMLTVNVLSESTTIRWKV
                     KEKALQFRHDTVFKAVVENATLTCYYQKDSTEIYNVYGSYYPEIQEFHGKKGIITWEK
                     AGYSREEVYAEISDFIISTAKDNFTIDSARLHHKTYFREPVYGVLYDKAIAFSQKEKA
                     DFPRFETYTKE"
     gene            complement(916..1683)
                     /gene="tpiA"
                     /locus_tag="PLZ75_01465"
     CDS             complement(916..1683)
                     /gene="tpiA"
                     /locus_tag="PLZ75_01465"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOS71098.1"
                     /translation="MRKKIVAGNWKMNMNYDEGLELAASLDQYFSEKAPSGAEVIICP
                     PFIHLAGISQNLKSGNVALGAQNCASEASGAFTGEVSADMIRSTGAEYVIIGHSERRS
                     YYNEDDSLLNKKTVLALNSGLKVIFCCGEIKEEREDGSYFEVVKRQIEKGLFPLAAKE
                     MKNIIIAYEPVWAIGTGLTATPDQAQEMHLHIRELVREKYGTEIADKLTILYGGSCKP
                     SNASDIFSRPDVDGGLIGGASLKKDDFTAIVEAMKQT"
     gene            complement(1696..2817)
                     /locus_tag="PLZ75_01470"
     CDS             complement(1696..2817)
                     /locus_tag="PLZ75_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOS71099.1"
                     /translation="MRYLLLICRITAGLVFIFSGTVKAIDPLGSAYKFQDYFLAFNMD
                     FLLPLSLPLGILLCAAEFFTGFSVLFNIRQKTGIWLLMLMMIVFTPLTLVLALTNPVT
                     DCGCFGDVIHLTNWQTFIKNIILLIPVTVLFINRNKVIPASGTIKEWMAMAGILALFI
                     AFSLFNLRYLPLIDFLPYKTGTYIPDKMKIPEGKPADRYETTFIYEKDGIAKEFSLEN
                     YPADDSSWVFVDQKTVLAEKGYEPPIHDFVLTTQDNTDITEDILASEDFTFLMICQKI
                     ENIKAGRLNEGFALGNRLSEENIQFLVLTASGAAEIMRYGSSHDVCSVDEITLKTMVR
                     SDPGYMLLRAGTILGKWSWARIPSFEYLEKEFLKTDNLN"
     gene            complement(2819..3505)
                     /gene="tmk"
                     /locus_tag="PLZ75_01475"
     CDS             complement(2819..3505)
                     /gene="tmk"
                     /locus_tag="PLZ75_01475"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOS71100.1"
                     /translation="MKLLVIEGVDGSGKSTQIKLLNDWFKNKGQECKYLHFPRTDSPF
                     FGELIARFLRGEFGSLNQVSPWLVAILYAGDRKDASGLLNEWLRSGSMVILDRYTFSN
                     IAYQCAKLNDEKEREELMKWILDLEFRHFGIPVPDLNIFLDVPFAFTEKKLLLSRTGD
                     DRKYLGGTRDIHEESLLFQKKVREVYLQLAGRDERLQIINCSNGQGIMLPPQKISELI
                     IDLLTDKKII"
     gene            3667..4515
                     /gene="folP"
                     /locus_tag="PLZ75_01480"
     CDS             3667..4515
                     /gene="folP"
                     /locus_tag="PLZ75_01480"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOS71101.1"
                     /translation="MLADKPGFINAGGKLIDLSVPKVMGIINVTPDSFYPGSRVTGNR
                     EIVESAAEMISEGADILDVGGYSSRPLADDVPAEEEKKRVLNAIRRIRERFPDAIISV
                     DTFRAEIAYEAVTGHGVQIVNDISGGDADGEMFGLIAELNVPYVIMHMKGNPRNMQDN
                     PEYEDVVAEILRWAGERINKLRRAGVRDIILDPGFGFGKTIKHNFDMLRRLGDFSIAG
                     LPLMAGISRKSMIWKTLSVSPGEALNGTSVLHAVALLRGVDILRVHDVKEAVQAVRLI
                     EMLKGS"
     gene            4545..5321
                     /gene="cdaA"
                     /locus_tag="PLZ75_01485"
     CDS             4545..5321
                     /gene="cdaA"
                     /locus_tag="PLZ75_01485"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOS71102.1"
                     /translation="MLVPVTVLDIIDILLVAFIMFQFYRIIKGTSVFSIVIGVFLIYM
                     FWLLVKALNMSLISSLLGQVIGVGVIALIVVFQQEVKRFLLVIGNRYIKNSKISFKRF
                     FSSEQDESVIPSVVEEIVKAAQSMSSKKIGALIVIGRQSMLGLYADRGQRIEADISAD
                     LLETIFFKNTPLHDGAVLIENNQILAARCPLPVTDQYALSSHFGMRHRAALGISEQTD
                     ALVVVVSEETGRISVADAGEIRENIKPDELRNILLMEKVW"
     gene            5392..6204
                     /locus_tag="PLZ75_01490"
     CDS             5392..6204
                     /locus_tag="PLZ75_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS71103.1"
                     /translation="MDVKGRVLITGGTSGLGLELVKLFLRKGFIVVTTGRRMLELPEH
                     EGRFIFYHVDFADLSATSELFGKISKTHEFDYIIYNAGILSPPDFTTTKDGFEYTFQI
                     NFLAHFLVNEIIIRHHAPDKPLRVVAVTSMAYRVAEPDFNYFGDAGNYRAWKAYSGSK
                     LYLVLMCRYFAGKLSGTPVRFYSYDPGIFGSELYRTQKGIFRRIYQLGVKILRHPAVS
                     AEVLAEILTDPDLTNGAVYDVRKRIRKVSLPGTVNSEAFWTAIHALARKYLK"
     gene            6286..6897
                     /locus_tag="PLZ75_01495"
     CDS             6286..6897
                     /locus_tag="PLZ75_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOS71104.1"
                     /translation="MKNTLISIMVLLSFSGLPGCREKDPSAWSSSKLDKWFEKGEWLN
                     GWHIKPDASVNRRELAVSYFRHRERWDKAFAFLRDNDPASMELKRYDLDGKNLYATVS
                     EYVSKDDSAALYEAHREYIDIQYVVSGRELIGIAPLNDVREIIVPYDEENDIEFMTVN
                     QIKNLTATPGRFFLFFPSDVHRPGLKDGENAAVRKVVVKVKIN"
     gene            complement(6978..8411)
                     /locus_tag="PLZ75_01500"
     CDS             complement(6978..8411)
                     /locus_tag="PLZ75_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOS71105.1"
                     /translation="MKSGRLFAFIIIILLFQSCRNTPEPAALFRILENEYENGNFQEI
                     PLLLDSLRTRFPNEEGLISMADSLMQISERIKLDFSLTEDQFIQRIEAYRIPVNDSIL
                     RVWDSKKWTEWRLIDGQKRYYRRAASNLRLLRMFYENRDAEKSPDPEDIATLRHTEGI
                     IAASEKTRDPVCPVSMTINYTIRVNPDVVPEGETIRCWMPWPRTDHLRQQKIEFLSAS
                     GKDFIFSPDSMIHKSVYMEQKARRGSPAVFSVSLRYESSGQFINPERLKPEPYNKESS
                     LYRKYTREEPPQIRFSEGVKKLADSLAAPDTGPFETARLFYMWFKENIPWTGALEYST
                     MADMPAYVLENMRGDCGMQTLLYISMLRYRGIPARWQSGWTVFPDHENLHDWSEIYFE
                     GTGWIPSDITYDLQASKDKTVREFFISGIDSYRLILNNGISGRLYPEKIHLRSEPYDF
                     QRGEVEWKGGNLYFDKWDYEISIEYGK"
     gene            8526..9431
                     /locus_tag="PLZ75_01505"
     CDS             8526..9431
                     /locus_tag="PLZ75_01505"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOS71106.1"
                     /translation="MIYNNILQTVGNTPLVRINRLISPGSVTISAKVEGFNPTGSIKD
                     RIALRMIEQAEAEGCLSNGRIIIEPTSGNTGIGLAMIGSVKGYRVEIVMSNAVSVERV
                     KMIRSFGATVTLTDSKAGTDGAILKARELVELHPDKYFMPDQFSNRFNKLVHYKTTGE
                     EIWKQTNGNIDYFVSAIGTSGTIMGVGSALKENNPGIKIVCAHPVKGHYIQGLKNMEE
                     AIVPSIYDPSLIDETIMVETEEACRMARKIIEKEGIFVGMSSGAAMCAAMRLAERIKS
                     GNIVVIFPDRGEKYLSTVLFNGENF"
     gene            complement(9539..9862)
                     /locus_tag="PLZ75_01510"
     CDS             complement(9539..9862)
                     /locus_tag="PLZ75_01510"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71107.1"
                     /translation="MNADRWSAFRKHEKEPFAGSKPSAGGDGGNHFANFLEALRAGKP
                     EMLNCEIIEGVYSAALVHLANISYKLGRSLRFINGYEKFANDPEADTLLSRDYRSPYI
                     VPAEV"
     gene            complement(9902..10948)
                     /locus_tag="PLZ75_01515"
     CDS             complement(9902..10948)
                     /locus_tag="PLZ75_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71108.1"
                     /translation="MTKRREFIRTTALGTAGITIGGLGLDARTYSGIKGANDRINLAL
                     VGIRNQGSEHINEWCALRKSHNVELTTLCDADERLFGPKAKTVTEKTGRKPKTEWDIL
                     KVLEDPEIDAVAIVTPNHWHALATIWACQAGKHVYVEKPASHNIFEGRKMVEAARKYN
                     VLVQVGHNNRSSRNVIEAIEFLHKGGIGEIYMARGLCYKSRDSYGWAKDSTPPPSFHY
                     ERWLGPAQWRPYNVKRSHYSWHWYWDTGNGDTGNQGPHQFDIARWGLSKYEHPVSVYS
                     TGGIYGFRKDESAPGTEIRGTMVYGDVETYGDSKTSQETPNIQTASFMYNDGTILEFE
                     TRGRYTNREGKSEI"
     gene            11058..11465
                     /locus_tag="PLZ75_01520"
     CDS             11058..11465
                     /locus_tag="PLZ75_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71109.1"
                     /translation="MKKNIEGTRQYLQLAADIGAIGILVFPNALPREGDMERKRILEQ
                     IGKSVSELATFGYNLGVQLRLEEQFNLVKGRIGNVHLRELFTGDPWRKLFNLLSESGY
                     EGYCDIELSEQSCELVRMMKNYRVGFLALQNAL"
     gene            complement(11559..13214)
                     /locus_tag="PLZ75_01525"
     CDS             complement(11559..13214)
                     /locus_tag="PLZ75_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS71110.1"
                     /translation="MKSNKNLQYMLAILIFGGTLFISSCDESSFLNRYPLDSPSPDVF
                     FVNETSARMAVTAAYESWRRGQYSYYRDMVIMNDALSDDSYWRPSRAAAIAFSQWQIN
                     SSDPIANDYWGIAFQSVNAANFAIENIPLLLDKGLTQEKIDPYIAEARFMRGFSYMFL
                     TSFFGDVPLHDRPRSSFEDFEQPRTSVADIYNQIIADFTFAKEKLPEKQPSSYTGAPT
                     KATAAAFLAKAYLYNKEWGPAEAAARSAINIAEAAGYGLEDSYEEIFKHENERNKELL
                     FYIQFIDNNRSFGDNYMIQRITRNCPPEFIHVYGMAGWGYALPTRDLFDAYEPGDSRR
                     EATIHYPGSVYGIYNGDKPFTYSHRTYNTEGNLISYEKNYTVGDKIDYDYRWSETGMN
                     VKKSTRNVAHLQDEYSDGLDVPIMRMAELYIILAEALAEQGKEEALTWVNKVRARPSV
                     NLPPKTQADGTLIDIVRKERRVELAMEGHRIFDLLRWGIMKQTFGDNKKVKKHFFSDY
                     LTDEFTRFSQPKLEKYPGETVLLPIPQNEIDRNSKIDKNNPGY"
     gene            complement(13249..16203)
                     /locus_tag="PLZ75_01530"
     CDS             complement(13249..16203)
                     /locus_tag="PLZ75_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS71111.1"
                     /translation="MAQQIPVTGIITDYQTGETIPGVNVMVKGTTIGTTSDIDGKYSL
                     TVPDPNAVLVFSFVGYSSLEQEISGRSTINVSLTPITQALDEVLVIGYGTVRRSNLTG
                     AVSQVRTGDFERVPTTSPLISIQGRSPGLSIVPNSGEPGSSASIRIRGDQSISGTNSP
                     IFVVDGVITDNINNLNSEDIESISILKDASVVAIYGARAANGVILITTKRGMAGHTPS
                     ITFHTYHGLQQTSNLRLKLLNAEQFLELWTEAYDNAGISKPWGEEELIKYKGVDEDWL
                     GAVMRTGYLANYNLSVSGGTERSNYFVSAAHLNNKGMVLGMDYSRFNLRFNGDYKIGQ
                     LIKFGNSVSVYSGKRNGSVGQYTRALQKVPITRIYEDDGGWGKIRNTSLEHMHGNAVW
                     MAETNSSKLDEKGIMGNLYLTISIIKGLDFTTRGNLEWNNNYQTDFTGGVDPLWQWEG
                     STINFVSKDNRETLHWITDYLLDFKRTFGTVHDVSALLGYSLEEQTYESLFASRTGTP
                     NNAIRFLNAGDPASQLNTNSYSDWAFASLFGRIGYTYSNKYIFSGTVRRDGTSRLSKE
                     KRYGIFPSVSFAWRVGQEKFMDSVSWLDELKLRVSWGLTGNVLSVGPYGTVPSLIARN
                     YPMNDQPSQGYTMSTAVNKNIKWENTEKKNLGLDITVLKNSLYLISDFYIQDTYDLLF
                     TQPIPGSTGLTGSPYINAGQIRNTGIELEIGYQKTIGKWYYSLSANATHFKNKVIDLA
                     GRDLRTEGIVEGYPLRSFFGYKSNGLIRTENDLKNNPQFPGKKIGDIWLLDIDGPDTQ
                     GKLTGKPDGVVNANDRTLIGSRYPDLTYGFMGTAGYKNWTLQVQLQGVQGILKDLRGG
                     TNLGVLNYFTMWAMNHDVLLLDRYHPTKNPDGIWPRVDKADSGNNVNMLSDFWLKNAS
                     YLRIRNININWDMPKSLVQKIRAGKLSTYISVQNLYTFTSFYGPEVDSNADVLTGVPQ
                     PRIWTLGFQLSF"
     gene            17796..17870
                     /locus_tag="PLZ75_01535"
     tRNA            17796..17870
                     /locus_tag="PLZ75_01535"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17831..17833,aa:Pro,seq:tgg)
     gene            complement(18313..18507)
                     /locus_tag="PLZ75_01540"
     CDS             complement(18313..18507)
                     /locus_tag="PLZ75_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71112.1"
                     /translation="MIVLDKDLKIRLLKAIKEGIFRGEDFPELATELKRIQIEVINSA
                     DQVRQDDEIVPGAILGAITS"
     gene            complement(18504..18809)
                     /locus_tag="PLZ75_01545"
     CDS             complement(18504..18809)
                     /locus_tag="PLZ75_01545"
                     /inference="COORDINATES: protein motif:HMM:NF039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HOS71113.1"
                     /translation="MVHSQFKPGQSGNPKGRPKGVKNKVTTETRAWLQKIVNDNRQQL
                     EVDLMRLDPRDRWNIIEKLLQYCLPKLSTIDASVQFEQLNEETIEQIVNELLQSIRK"
     gene            complement(18836..19051)
                     /locus_tag="PLZ75_01550"
     CDS             complement(18836..19051)
                     /locus_tag="PLZ75_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71114.1"
                     /translation="MDEIKLSRDYVAAFRAAYAIKNIDEMFNIFQSLLSEIGIPDSSY
                     IAAQQAYGKAAAELLNARDRGIGGKKI"
     gene            complement(19069..19272)
                     /locus_tag="PLZ75_01555"
     CDS             complement(19069..19272)
                     /locus_tag="PLZ75_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71115.1"
                     /translation="MKKKSKSTIWPRMKTFDEKAKGKRGKLAVLEGMNPFPKEPFAIT
                     MQKAFMSNHLDATKVYFVSPEEE"
     gene            complement(19269..20066)
                     /locus_tag="PLZ75_01560"
     CDS             complement(19269..20066)
                     /locus_tag="PLZ75_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71116.1"
                     /translation="MTGRNKRIDLRASQKDEKNLQAVAEKLGTKTITDTIFQAVETVA
                     TDEPELFLIDRERIRVLDEMSEFSKKHFQAFIDEFRKVTGWDITMTQLKQIFKHTGTL
                     GSKAKLQNEINNMIRAGQLARMQAKYPDLIVTFDNIPDLETSELLRLAEKFEDMPTFV
                     PGAKPVIYWNTIELKEGKINILSAEVEKLKNSYRHYATTPEEKKKLAKVKKLCQALND
                     ILATGEVNPDGISRLFYYDAIAARFEPTGGWIKFSVNPHDIIKIDES"
     gene            complement(20371..20949)
                     /locus_tag="PLZ75_01565"
     CDS             complement(20371..20949)
                     /locus_tag="PLZ75_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71117.1"
                     /translation="MMSNYLTKLWCSDPERFRDIIAKEPFDEELYLMSVIDEDGSVYI
                     ESCLHTPKEIICQLSADNPDLIFTAELSFEIEEFEIIYTYNFKNGECAHVKTEPNYNI
                     CYSERDGDPEFRYRGIIGEYYYDKLFNRVIEICKRLDIVTDNGVDFVQEKIQITVEDS
                     NIIMTVLKHGSRLEVMDCYKREFEISDSLPFF"
     gene            complement(21241..>21688)
                     /locus_tag="PLZ75_01570"
     CDS             complement(21241..>21688)
                     /locus_tag="PLZ75_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71118.1"
                     /translation="VTSGKFYQYMMNKFKENDISFIDRKDLKEQLFIVFFGRNGAERY
                     SKAARLFSKCFPNVWQLFKTIKKEDYRKLAILLQKIESFVILQRVAPRIVQDLPGVPF
                     ITKHDSILPSDILIVREAGKVKKIMAEVIKEVTGYCPKIRIKNHEN"
BASE COUNT         5879 a         4546 c         4549 g         6714 t
ORIGIN      
        1 aactcctttg tatatgtctc aaatctcggg aagtcagctt tttctttctg actgaacgca
       61 atcgccttat cataaagcac tccgtagaca ggttccctga aatatgtctt gtgatgaagc
      121 ctggcagaat cgatggtgaa attgtccttt gccgtactga taatgaaatc gcttatttct
      181 gcatagactt cttcacgtga ataacctgcc ttttcccatg ttattattcc tttcttcccg
      241 tgaaactcct ggatttccgg ataataggac ccgtaaacgt tatagatttc agtactgtcc
      301 ttctgataat aacaggtaag ggtggcattt tccacaactg ccttgaaaac tgtgtcatga
      361 cggaattgaa gagctttctc cttgaccttc catctgattg tggtactttc ggaaagaaca
      421 tttaccgtca gcatcagctc agtgtccctt atgtacctgt ttattttgtc aacggatatc
      481 ctgggatcaa agacaatttc gctgagtcct ttgagccagt tgctcaggaa ttccctgtca
      541 ttgccggccc tgatataggt attgagagtg acaagaaagt tgtacatatg cgggacaggc
      601 ctcattgatc ttccgtaaaa ctggctggaa acgtcgatta tcctggtcat atcctcagtg
      661 tcaaatttct ggctttccca gttttgcaga aagaggttca gttcctcctt gtgattctcc
      721 ttcaggttag ggcccatgaa agatatgagc tgttccctgt attttgaggg atcgactgaa
      781 aagggtgaag tgagctgtgc atgcaactcc ccgggtaaaa caatcaggag tatcagggtt
      841 gtgatgacca gtaatcttga catgattgca gaattctctt tcctgttatc tcatgcaaca
      901 atcttcacaa ttgaattatg tctgtttcat tgcttcaact attgctgtaa aatcgtcttt
      961 cttgagagat gcaccgccta tcagtccgcc atcgacatca ggccgggaaa aaatgtctga
     1021 ggcatttgac ggtttgcagc ttcctccata cagaatcgtg agtttatctg caatttccgt
     1081 accgtatttt tccctgacca gttcccggat atgcaggtgc atttcctgtg cctgatccgg
     1141 cgtggcggtc agaccggtac cgatagccca tacaggttcg taggcgatta ttatgttttt
     1201 catttctttt gctgcgagcg gaaacaatcc cttttcaatc tgccttttca caacttcgaa
     1261 ataagatcca tcctctctct cttcctttat ttcaccacag cagaaaatca ccttaagtcc
     1321 gctgttaagc gcaaggacgg tttttttatt cagcaggctg tcatcctcat tataatagct
     1381 ccttctttcc gaatggccga taattacata ttcagccccg gtgctcctga tcatatctgc
     1441 agatacttca cccgtaaatg ctcctgaggc ctccgatgca caattctggg cacccagtgc
     1501 cacattcccg cttttcagat tctgtgagat gcctgccagg tggataaaag gcgggcatat
     1561 tatcacctca gccccggaag gagctttttc agagaaatac tgatcgaggg atgcggctaa
     1621 ttccagtccc tcgtcatagt tcatgttcat tttccagttt ccggcaacaa ttttctttct
     1681 catttgaaat agtgtttagt ttaaattatc agtttttaaa aattcttttt caagatattc
     1741 aaatgaaggt atcctggccc atgaccattt gccgaggatt gttcctgccc gcagcagcat
     1801 atatcccgga tctgatctta ccattgtctt gagggttatc tcgtctacac tgcacacatc
     1861 gtggctgctg ccgtatctca tgatctctgc ggctccggat gcggtaagaa ctagaaactg
     1921 aatattttct tctgacagcc tgtttccaag cgcgaaccct tcattcagtc ttccggcttt
     1981 gatgttttca attttctgac agatcatgag gaaggtgaaa tcctctgatg caagtatgtc
     2041 ttcagtaatg tccgtattgt cctgtgtggt aagtacaaaa tcgtgaatag gaggttcata
     2101 gcccttctca gccagtacgg ttttctgatc cacaaatacc caggagctat cgtctgcagg
     2161 gtagttttca agtgaaaatt cctttgcaat accatctttc tcatagataa aggttgtttc
     2221 atacctgtcc gcaggttttc cttccggaat tttcatttta tcaggaatgt aagtgccggt
     2281 tttgtatgga aggaaatcaa ttaggggaag atatctgaga ttgaaaaggg aaaaggcaat
     2341 gaagagggca agtattcctg ccattgccat ccattccttt attgttccgc ttgctggtat
     2401 aaccttgttt ctgtttataa acagaactgt taccggaatg agcagaatta tgttcttaat
     2461 gaatgtctgc cagttggtaa gatgtataac atcgccgaag catccgcagt ctgttacagg
     2521 atttgtaagg gccaggacaa gggttagcgg tgtgaaaaca atcatcatga gcataaggag
     2581 ccagattccg gttttttgac ggatattgaa caaaaccgaa aagccggtaa aaaattcagc
     2641 tgcacacaga agtatgccga gcggcagact taaaggaaga aggaaatcca tgttaaatgc
     2701 caggaagtaa tcctggaact tgtatgctga acccagtgga tcaatggctt ttaccgtgcc
     2761 tgaaaaaatg aagaccaggc cggccgtgat cctgcatatc agtaacaagt atctcatatc
     2821 atattatttt tttgtctgtt agtaagtcaa ttattagttc agatattttt tgtggaggaa
     2881 gcattatacc ctgcccatta ctgcagttta taatctgaag ccgttcatcc ctgccggcaa
     2941 gctgaaggta tacttcccgt acctttttct ggaaaagaag tgattcttcg tgaatatccc
     3001 tggttccgcc aagatatttc ctgtcgtcac cggtcctgga aagcaataat tttttctcgg
     3061 taaaggcaaa cggcacatcg agaaaaatgt tcagatcagg aacaggtatt ccgaaatgcc
     3121 tgaattccag gtccagtatc catttcatca gttcttcacg ttccttttcg tcattcagtt
     3181 ttgcgcactg gtaggcaata ttcgaaaagg tgtacctgtc aagtatgacc atgctgcccg
     3241 atctcagcca ttcatttaat aatcctgatg catcctttct gtctcctgca taaagtattg
     3301 ccacaagcca gggactgacc tgattcagac tgccgaattc cccgcgcagg aagcgggcaa
     3361 taagctcccc gaaaaatgga gaatcagtcc tgggaaaatg aaggtatttg cattcctgac
     3421 ctttattttt gaaccagtca ttcagaagtt ttatttgtgt ggattttcct gatccatcaa
     3481 ctccctcaat aacaagtaat ttcatctcac agtcattagc agtagtgcaa atgtagcaag
     3541 cgcacttcaa agattaaaac gaaaggccgg tataattatg atttgttttc atgagttatt
     3601 aaccggatgc gtgcagaggt gtttgctttt aatgaaaaat cactaatttc acaaaataat
     3661 cctacagtgt tggctgataa accgggtttt attaatgcag gcgggaaact cattgatctg
     3721 agcgttccta aagtgatggg gataatcaat gtcactcccg attcattcta tccgggtagc
     3781 agggttacag gaaacaggga gattgttgaa tcagcggctg aaatgatcag tgagggtgcg
     3841 gacatacttg atgtcggagg ctattcttca cggcctttag ctgatgatgt gccggcggaa
     3901 gaggagaaaa aaagggtgct gaacgcaatc agaaggataa gggaaaggtt tccggatgcc
     3961 attatctcag ttgatacatt ccgggctgag attgcatatg aggccgtcac cggtcatggt
     4021 gtacagattg tgaatgatat ttccggcgga gatgccgacg gggaaatgtt cggtttaata
     4081 gctgaactca atgttcccta tgtgattatg catatgaagg gaaatccgcg taacatgcag
     4141 gacaatcccg agtatgagga tgtggttgca gagatactga gatgggcggg tgagaggata
     4201 aataaactca ggagggcagg agtaagggat ataatcctgg atccgggttt tggttttggt
     4261 aagacaataa agcataactt tgacatgctt aggcgcctgg gtgatttttc cattgccggc
     4321 ttgccgctta tggcggggat ttcacggaaa tcgatgatct ggaaaaccct ttccgtatct
     4381 cccggtgaag cacttaacgg cacgtctgtt cttcatgcag ttgccctcct cagaggggtt
     4441 gacatactca gggtgcatga tgtgaaagaa gctgttcagg ctgtcaggct gattgaaatg
     4501 ttaaaaggaa gctgaactgc gaaagataaa caaacaatgg taagatgtta gtaccggtaa
     4561 ccgttcttga tataattgac atactgcttg ttgctttcat catgttccag ttttacagga
     4621 tcatcaaggg aacttcggta ttcagcattg taatcggcgt attccttatc tatatgtttt
     4681 ggcttttagt caaagcactt aatatgagcc ttataagttc tcttcttgga caggtgatag
     4741 gagtcggtgt gattgccctg atagttgtat tccagcagga ggtaaagcga tttctgctgg
     4801 tgatcgggaa cagatatatc aagaattcaa agatttcatt taagaggttt ttctcttcag
     4861 aacaggatga gtctgtcata ccttcggttg tggaggagat agttaaggct gcccagtcga
     4921 tgagttcgaa gaagatcggg gcactgatag ttatcgggcg gcagagtatg ctgggtttgt
     4981 atgccgaccg cggacagagg atagaagctg acataagtgc tgatctcctt gaaacgatat
     5041 ttttcaagaa cactcctctt catgacggag cggtactgat agaaaacaac cagatactgg
     5101 ctgccaggtg tccgctgccc gtgacggacc agtacgccct gtcatctcat ttcggaatgc
     5161 gtcacagagc tgctctggga atatcagaac agacagacgc gcttgttgtg gtagtatctg
     5221 aggaaacagg gcggatttcc gtggctgatg caggtgagat ccgggaaaat atcaagcctg
     5281 atgaactgag aaacattctc cttatggaga aggtatggta aaaatttcca cattttcccg
     5341 gctcagacgg cctttttatt atgtttgtcc gttaattttg gatgaactca tatggatgtg
     5401 aagggcaggg tgctgattac aggcggaacc tcaggactgg gtcttgaact tgtgaaattg
     5461 tttctccgga aaggatttat tgttgttacc acagggagaa ggatgcttga gttaccggaa
     5521 catgagggca gattcatctt ttaccatgtt gattttgcgg atctttccgc tacttcggag
     5581 ctgttcggaa aaatctccaa aacacatgaa tttgattaca taatttataa tgcagggatt
     5641 ttaagccctc ctgacttcac aaccactaag gacggatttg aatatacatt ccagattaac
     5701 ttcctggcac attttcttgt gaatgaaatc attatccgtc atcatgcccc tgacaaaccg
     5761 ctgagggtag ttgctgttac ttccatggca taccgggtgg cagaaccgga ctttaattat
     5821 ttcggggatg ccgggaatta ccgggcatgg aaagcttatt ccggttccaa gctttatctt
     5881 gttcttatgt gccggtattt tgccgggaaa ctttcgggaa ccccggtccg gttttacagt
     5941 tatgatcccg gaatttttgg ctccgagctt taccggacac aaaaggggat tttcaggagg
     6001 atttaccagc tgggggtgaa aatacttaga catccggccg tgtcagcaga agttcttgct
     6061 gaaattctga cggatcctga tttaactaac ggagctgtat atgatgtaag aaagagaatc
     6121 cggaaagtca gcctgcccgg aactgttaac agtgaggcat tctggaccgc catccatgcc
     6181 cttgcccgta agtatttaaa ataaagaggc agaaatggtt gttttttttt ataatttgcc
     6241 attggtattt gtcagagagt attttttaaa aattttaaac aggaaatgaa aaacacatta
     6301 atctctatca tggtactatt aagcttttcc ggtttgcccg gctgcaggga aaaggatcct
     6361 tcagcctgga gcagcagcaa acttgacaaa tggtttgaaa agggtgaatg gctgaacgga
     6421 tggcatataa agcccgatgc ttctgttaac cgcagggaac tggcagtctc atatttcagg
     6481 cacagggaaa gatgggacaa ggcttttgct tttctcaggg ataatgatcc cgcatcaatg
     6541 gaactgaaac gttatgacct tgacggaaaa aatctgtatg ctactgtcag tgagtatgtc
     6601 tcaaaagatg attctgccgc cctctatgaa gcacacaggg aatacatcga cattcagtat
     6661 gttgtgagcg gaagggaact gataggaatt gcgcccttaa atgatgtccg ggaaattatt
     6721 gtcccttatg acgaagaaaa cgatattgaa tttatgaccg ttaatcagat taaaaacctt
     6781 actgccacac ctggcaggtt tttccttttc tttccgtcgg atgttcaccg gccgggactg
     6841 aaggatggtg aaaatgcagc tgtcagaaaa gtggtagtca aggtaaaaat caattagacg
     6901 aaaaggtcag ccggaactcc ctgtcctgct tccgctatgt tgcatccgcc aggccacggc
     6961 agatattcag tatcagacta tttcccgtat tctatactta tctcgtagtc ccatttgtcg
     7021 aaatacagat tcccgccttt ccattcaact tctcctctct gaaaatcata gggttcactt
     7081 ctgagatgaa ttttttcagg atacagtctg ccggagattc cattgttcag gatcagtctg
     7141 taggagtcga ttcccgagat gaaaaattcc cttacggtct tgtctttgct tgcctggaga
     7201 tcatatgtga tatccgacgg tatccatcct gtgccctcaa aatagatttc actccagtca
     7261 tgaagatttt cgtggtcagg aaaaactgtc cagccgcttt gccatcttgc aggaattccc
     7321 ctgtatctga gcattgaaat atacagcagt gtctgcattc cgcaatcgcc tctcatgttt
     7381 tccagaacat aggcaggcat gtcggccatt gttgaatatt ccagcgcacc cgtccatggg
     7441 atgttttcct tgaaccacat gtaaaacagc cttgcagttt caaaaggacc ggtatcagga
     7501 gctgcaagac tgtctgcaag tttctttaca ccttccgaga aacggatctg gggcggttct
     7561 tcccgggtat attttctgta aagcgaagat tctttattgt atggttcagg ttttaagcgc
     7621 tcgggattta tgaactgacc tgatgactcg tagcgtaatg atactgaaaa gactgcagga
     7681 cttcccctgc gggctttttg ttccatgtag actgatttgt ggatcatgga gtcgggagaa
     7741 aaaataaagt ccttcccgga ggctgacagg aattcgattt tctgctgccg caggtgatct
     7801 gttctgggcc atggcatcca gcagcggatt gtctctccct ccggtacaac atccggattg
     7861 acacggattg tataatttat tgtcatgctg accggacaga caggatcccg ggttttttcc
     7921 gatgctgcaa tgatcccctc tgtatgtctg agcgttgcaa tatcttccgg atcagggctt
     7981 ttttctgcat ccctgttttc ataaaacatc ctgagcagtc ttaaatttga tgctgccctt
     8041 ctgtaatacc tcttttgccc gtcaataagc cgccattcgg tccatttctt actgtcccac
     8101 acccggagga tgctgtcgtt gaccggaatc ctgtaagcct caattctctg aatgaactgg
     8161 tcttcggtaa gagaaaaatc aagttttatc ctttcggaaa tctgcatcag gctgtctgcc
     8221 atcgagataa gaccttcttc attgggaaac cgggttctca gggaatcaag gagaagcggt
     8281 atctcttgaa aatttccgtt ttcatattca ttttccagaa ttctgaataa agctgcgggt
     8341 tccggtgtat tcctgcagga ctgaaacaac aggataataa tgatgaaagc aaaaagccgg
     8401 ccggatttca tagaattggt aattattatc ttataaggta aaaatagtta tatttttaag
     8461 gtattggaaa agaaaagtaa taatattttt aaatttgcgc tgctgatttt aaaaattaaa
     8521 aaacaatgat ttataataat atattgcaaa cggttggcaa tacgcctctt gtaaggataa
     8581 accgtcttat ttcacccgga agtgtcacga tcagtgcaaa ggttgaagga ttcaatccaa
     8641 cgggcagtat caaggacagg atagccttgc ggatgattga gcaggctgag gcggaaggat
     8701 gcctcagtaa tggcaggatt attattgaac cgacttccgg gaacacgggt attggactgg
     8761 ccatgatagg gtctgtcaag ggctacaggg tggaaatagt gatgagcaat gccgtttctg
     8821 tagaaagggt aaaaatgatc aggtcattcg gggccactgt tacacttacc gattcaaagg
     8881 caggtaccga cggagccatc cttaaagcaa gggaacttgt ggaacttcat cctgataaat
     8941 atttcatgcc cgaccagttt tcaaacaggt tcaacaagct ggttcactac aaaacaaccg
     9001 gtgaggaaat ctggaagcag acgaacggta atatagatta cttcgtttcg gcgattggta
     9061 catcaggaac aataatggga gtgggaagcg ccctgaagga gaataatccc ggtataaaga
     9121 tcgtctgcgc ccatccggta aagggccatt atatccaggg tctgaaaaat atggaagagg
     9181 ccatcgtgcc atccatttat gacccctcac tgattgatga gaccattatg gttgaaactg
     9241 aggaagcctg caggatggcc cggaagatta tagaaaagga gggaatattt gtggggatga
     9301 gcagtggtgc cgccatgtgt gctgccatga gactggcaga aagaattaaa tcgggaaata
     9361 ttgttgtaat atttcccgac aggggcgaaa agtatctcag tacagttctt tttaacggag
     9421 aaaattttta aaacggataa tctgttgttt ttccggctcc gggtaatccc tccccgttca
     9481 ttctgtcgca ttaatccgga gcctttttac agctgtacgg ggatgagtga cttatatcct
     9541 atacttcagc cgggacaatg tagggggatc tgtaatccct cgacagcaat gtatcagctt
     9601 ccgggtcatt ggcaaatttt tcgtatccgt ttataaatct gagactgcgt ccaagcttgt
     9661 agcttatgtt tgccaggtgc accagtgcag ctgaataaac cccttcaatt atttcgcagt
     9721 tgagcatctc cggttttccg gccctaaggg cctccagaaa attggcaaaa tgattcccgc
     9781 catcgcctcc ggcagacggt tttgatcctg caaatggttc tttttcatgt ttgcggaaag
     9841 cactccatct gtctgcatta atttcgagat atccttccgt gccgtaaaat atattaccga
     9901 tttaaatttc gcttttacct tcccggtttg tataacgccc tcttgtttca aactcgagaa
     9961 tggttccgtc attatacatg aatgaggcag tctggatatt aggtgtttcc tgggaagttt
    10021 tgctgtcgcc atatgtttca acatcaccat aaaccattgt tcccctgatt tctgttcccg
    10081 gagcgctttc atccttcctg aatccgtaaa tccctcctgt ggaatatacc gatacaggat
    10141 gctcgtattt gctgagtccc cagcgtgcta tgtcaaactg gtgcggtccc tgattgcctg
    10201 tatctccgtt gcctgtatcc cagtaccagt gccaggaata atggcttcgt tttacattgt
    10261 acggcctcca ctgggccggc cccagccagc gttcataatg gaatgaggga ggtggtgttg
    10321 aatcctttgc ccagccgtat gaatcgcgtg acttgtaaca gagtcccctg gccatgtata
    10381 tttcgcctat gcctcccttg tgtaggaact caattgcttc aatcacattc cttgaagaac
    10441 ggttgttatg tccgacctgg acaagaacat tgtattttct agcagcttcc accatcttcc
    10501 ttccttcaaa tatgttatga ctggcaggtt tttcaacgta gacatgcttt cctgcctgac
    10561 atgcccagat ggtagccagc gcatgccagt gattcggggt tacaattgcg acagcatcaa
    10621 tttcagggtc ttcgaggacc ttgagtatgt cccattcggt tttgggtttc cggccggttt
    10681 tttcggttac tgtctttgct ttcggcccga aaaggcgttc gtcagcatca cataatgtgg
    10741 tcagttcaac attgtggctc tttctgagtg cacaccattc gttgatatgt tcggaaccct
    10801 gattccggat tccgacaagt gccaggttta tacggtcatt tgcgcccttt atgccggagt
    10861 atgtacgggc atcaagcccc agacctccga tggtaatgcc cgctgtgccc agtgcggtag
    10921 tcctgataaa ttcccttctc tttgtcataa agcagaatta atgatttatt agcagttagt
    10981 gtaaaattaa ttatttatca tgaattagtc ataataaagg gcagtgatac tgaaatgcac
    11041 ttgccgatcc tacggagttg aaaaaaaata ttgaaggaac cagacagtat cttcagctgg
    11101 cggcagatat cggggccatt ggaattcttg tatttcccaa cgcactaccg cgggaaggag
    11161 atatggaaag gaaaagaatt cttgaacaga tcggaaaatc tgtttctgaa ttggcaacat
    11221 tcggttataa tcttggagtt cagctccgcc ttgaagaaca gtttaatctg gttaagggcc
    11281 ggataggcaa tgtgcatctg cgcgaattat tcaccggtga tccctggcga aaacttttca
    11341 acctcctaag tgaatcagga tatgaaggat attgtgatat tgaactctca gaacaaagct
    11401 gtgagcttgt caggatgatg aaaaattacc gcgtgggttt tcttgcgctg cagaatgcat
    11461 tataggaaaa ctgcattaaa aaatgtcagg aagcttctta gaaccttcct gacattcagt
    11521 aataaaataa ttgtttacgg attggatatc tgaattccct aataaccggg attattttta
    11581 tcaatctttg aatttctgtc tatttcattt tgaggtattg gcaataagac agtttccccc
    11641 ggatattttt caagttttgg ctgtgaaaac cgtgtaaatt catcagtcag ataatcagaa
    11701 aagaaatgtt ttttgacttt tttattatct ccgaaagttt gtttcattat tccccacctc
    11761 agcagatcaa aaatccgatg tccttccatt gcaagttcaa cacgccgctc ttttctgaca
    11821 atatcaatga gggttccgtc tgcctgagtt ttgggtggca gattaacaga cggacgggcc
    11881 cggactttat ttacccatgt cagggcttct tcttttccct gttctgcaag agcttcagca
    11941 agaataatat ataattcagc cattcgcatt atgggaacat ccagtccgtc tgaatattcg
    12001 tcctggagat gggcaacatt acgtgttgac ttttttacat tcattccggt ctctgaccac
    12061 ctgtagtcat aatcaatctt atcaccaaca gtataatttt tttcatagct tatcaggttt
    12121 ccttccgtat tatatgttct gtgtgagtat gtgaatggtt tatccccatt atatattccg
    12181 taaacagatc ccggataatg aatagtggct tcgcggcgtg aatcacccgg ttcatatgca
    12241 tcaaacagat cacgggttgg caatgcataa ccccagccgg ccattccgta cacatgaatg
    12301 aattccggag ggcaattgcg tgttatcctt tgaatcatat aattatctcc aaaacttctg
    12361 ttgttatcaa taaactgaat ataaaacagc aattccttgt ttctttcatt ttcatgtttg
    12421 aatatttctt cataagaatc ttcaagtccg tatccggctg cttccgcaat gtttattgca
    12481 gaacgtgctg ctgcttctgc cgggccccat tccttgttgt ataagtaagc ctttgccagg
    12541 aaagcggctg ctgtagcttt tgtaggagca cctgtgtatg aagatggttg tttctcgggg
    12601 agtttttctt tagcaaatgt aaagtcagca attatctgat tatagatatc agcaactgaa
    12661 gtcctgggct gttcaaaatc ctcgaaagaa gaacggggcc ggtcatgcaa aggcacatcg
    12721 ccaaagaagc ttgtcagaaa catataggaa aaaccacgca taaaacgggc ctccgcaata
    12781 taaggatcta tcttttcctg agtcagtccc ttgtcgagca aaagaggtat gttttcaatt
    12841 gcaaaattag cagcattgac actttggaat gctattcccc agtaatcatt tgctatggga
    12901 tcactggaat tgatttgcca ttgtgaaaaa gcgattgcag cagcccgtga aggtctccag
    12961 taggaatcat ctgaaagtgc atcgttcatt attaccatat cccggtaata actgtactga
    13021 cctcttctcc aggattcgta tgctgccgta acagccattc tggctgaggt ttcattgaca
    13081 aaaaatacat caggagaggg cgaatcgaga ggatagcgat ttaaaaaact ggattcatca
    13141 caggaggaaa taaaaagcgt tcccccaaaa atgaggatcg ccagcatgta ttgtagattt
    13201 ttattacttt tcattttact gtaattattg aattattgca tgaattgttt agaaagacag
    13261 ctgaaatccc agtgtccata tccttggttg agggacacct gtaagtacat cagcatttga
    13321 atcaacttca ggaccataaa aactggtgaa agtatacaaa ttctgtacac tgatgtaggt
    13381 tgacagtttt ccggccctga ttttttgaac caatgattta ggcatatccc agttaatatt
    13441 gatatttctt attctaagat aagaagcatt cttcagccag aaatccgata acatgtttac
    13501 attgttgcct gaatcagcct tgtccactct gggccagatt ccgtcaggat tttttgtggg
    13561 atggtagcgg tcgagcaaga gtacatcatg attcattgcc cacattgtaa agtaatttaa
    13621 gactcccaga ttggttcctc ccctcaaatc cttcagtata ccctgaacac cctgaagttg
    13681 tacctgtaat gtccagtttt tgtaacctgc agttcccata aaaccatagg tgagatcagg
    13741 atatctgctg ccaatgaggg ttctgtcatt agcgttgact actccatcag gcttaccggt
    13801 taattttccc tgagtatcag ggccgtctat atccagcagc catatatctc caatcttttt
    13861 gccagggaat tgaggattgt ttttcaaatc attttcagtt ctgatcaggc catttgattt
    13921 atagccaaaa aatgacctca ggggataacc ttccacaata ccctcggttc ttagatctct
    13981 tccggcaagg tcgataacct tgttcttaaa atgagttgcg ttggcactca ggctgtaata
    14041 ccacttgcca atagttttct gatagcctat ctcaagttct atccccgtgt tccgtatctg
    14101 tccggcgttt atatatggac ttccggtaag gcctgttgat ccggggatgg gttgggtaaa
    14161 taaaagatca tacgtgtcct ggatgtagaa atcagatata agatataaac tgttttttaa
    14221 gacagtaatg tcaaggccaa gattcttttt ctcagtgttt tcccatttaa tatttttatt
    14281 aacagcggtt gacatagtat aaccctggga aggctggtca ttcatcgggt aattacgcgc
    14341 tattagtgaa ggaacagttc cataaggacc tacgcttaaa acattacctg tcagtcccca
    14401 gctgactctt aattttaatt cgtcaagcca gctcacagaa tccatgaact tttcctgtcc
    14461 gacacgccat gcaaaggaaa cagaagggaa gataccgtat ctcttttcct tgctcagccg
    14521 tgatgttcca tcccgtctta ctgttcctga aaagatgtat ttattactgt atgtatatcc
    14581 gatgcggcca aataatgatg caaatgccca gtcggagtat gaatttgtat tcagttgtga
    14641 ggccggatct cctgcattaa gaaaacggat tgcattattg ggagtgcccg tcctgctggc
    14701 aaataaactt tcatatgtct gttcttcaag tgaatatccc aaaagggcgg aaacatcatg
    14761 cacggtacca aaagtcctct tgaaatccag taagtaatca gttatccaat gaagagtttc
    14821 ccggttatcc ttacttacaa agttaatggt acttccttcc cattgccata atggatcaac
    14881 ccctcctgta aaatccgtct ggtaattatt attccactcc agatttccgc gtgttgtaaa
    14941 atccaggcct tttataatgg agattgtcag atataaatta cccattatcc ctttttcgtc
    15001 aagtttgctg gagtttgttt ctgccatcca gactgcgttc ccatgcatgt gttcaagtga
    15061 agtattacga atttttcccc agcctccgtc atcttcataa atccttgtta tgggtacttt
    15121 ttgcaaagcc cgtgtatact gcccgacact cccgttacgt tttcctgaat agacgctgac
    15181 ggaattgcca aattttatta gttgtcctat tttataatca ccattgaatc tcagattgaa
    15241 tctgctgtag tccattccga gcaccatacc cttattgtta agatgagctg cagaaacaaa
    15301 ataatttgat ctttccgttc ctccagacac tgaaagattg taatttgcca ggtagcctgt
    15361 acgcatcacc gctccgagcc agtcttcgtc cacgccttta tactttataa gttcttcttc
    15421 accccaaggt ttggaaatac cggcattatc atatgcttct gtccatagtt ccagaaactg
    15481 ctcagcattc aataatttta gtctgaggtt acttgtttgc tggagaccat ggtaagtgtg
    15541 aaaagtaatt gaaggtgtgt gaccggccat tccccgtttg gtagttatga ggatcacgcc
    15601 atttgctgcc ctggcaccat atattgcaac tacggatgca tctttgagaa ttgaaatgga
    15661 ttctatgtct tcactgttca ggttatttat gttatcggtt attactccgt caacgacgaa
    15721 aatcggactg ttggtaccgc ttatggactg atccccgcga atgcggatac tcgcagaaga
    15781 acctggttct cctgaatttg gaacaatact taaaccaggc gaccggcctt gaatggaaat
    15841 taagggagaa gtggttggaa cccgttcaaa atcacctgtt ctgacctggg aaacagcacc
    15901 tgtcagattg cttcttctga cagtaccgta accaatgaca agtacttcat ccaaagcctg
    15961 ggtaataggt gtaagactca cattaattgt tgaccgacct gaaatttctt gttccagact
    16021 gctgtaaccg acaaaagaaa atacaagcac agcatttggg tctggaacag ttaaagaata
    16081 tttcccgtca atatccgatg tggtcccgat agttgttcct tttaccatga cgttaacccc
    16141 cggtatcgtt tctccggtct gataatccgt aataataccg gttaccggta tttgttgtgc
    16201 catcgaattt aaatgaagga ataaaataat accggtatat gcaattttac aggtattcct
    16261 ccataaattc ttagtaaaat tcaatttttg catctcgttt ttcataggaa ttgtttttga
    16321 ttagtaatag atgtttgggt cgtatcgtat gtatatacag gagagaagtg gacagaagtt
    16381 cagtcctgtc aggttttcaa agaaaatcaa tatccgattc aatttatcat atccgtttta
    16441 aaacaagcat cagaacaaaa gccttttttc ttcaacggtt tacagtgaaa attcaagaac
    16501 aggtatatta atggtttttc tactatcagt ctggagaaaa tacaaaataa ttcaatcagg
    16561 taagaaatcg tgattttatt cccgtaaaac aaatcctccc caaactcatt tttctgaaaa
    16621 agattttatc ggtaactctt atgtgaatat aatttttttc gatgtttaat acaaattttt
    16681 tatgcctttt ctttcagaaa tattcccgct taaatccgta ctccccctac taagaacact
    16741 ggttttgaat ttaatctgtt aaaatgtaat taatgaagtg attatgaatt acttttatac
    16801 tttttacagt tggaaaattc tggagaaaat actaaatttg atttgaatta ctgagggtaa
    16861 aaacggatga cagtatcgcc tgagtacccg gaatatttat ttaaccagtc agtcctggaa
    16921 atcattcata ctcttgatat ttaacagtat atgctaaaaa tgtgatttat tattctgcag
    16981 cttttttgcc agatttgtta aaaagcctgc agaaatgacg taagcaactg caaactagcc
    17041 gcattgtggt atcccgggca gtgaagaagc tctggcttct gcttctgcta gtgagaaata
    17101 acagaggtaa cttcctgcaa atttaatttg atctgatctg atctgatctg atccttggat
    17161 ttccatatcc tgattgtatc cctctttcaa ttgacagata aggtaagaaa ttaaggtttg
    17221 actaattgct ttcaaaaatc aattaaatga aaagagttat tggattttta ttttgttggt
    17281 aattatgtta gtgacataca tgtcatgcaa ttacaaggca cctgatccaa atgctccgaa
    17341 agcatgcttt acagcaccaa aggaaatatt tatcgggatt gctgccgcgt ttaactcgtc
    17401 atgtcctgag aacgctgtct ggcttaaaca tgctattcca tatataataa cggtaaacat
    17461 tagtgtaacg aacccgaccg gagcaaaact gacgatacag gcagggaaat acattcagca
    17521 ataacgccat gggcggcatt cacaatgagt aatgcctgat aatctggtag aagggactgt
    17581 ctgaaaatgt tccggacagc cactttcttt tctgctttca ccccgattgg tacagaagta
    17641 attgttcccg gtttcggcat agacaagaat gcttttttgc agccggaatg aatttttgaa
    17701 aactgaatat tcattacaca ggattctaag gaaaaactga ttatatttgc ggcactttta
    17761 aaacctgccg tcagtaatgc tgacgggcag ccttgcggga tgtagcgcag cccggttagc
    17821 gcatctggtt tgggaccaga gggtcgtcag ttcgaatctg accatcccga caaaagaaaa
    17881 tcagggagtt agcacgtgtt taactccctt tttatttatg gcattttcaa agaatttgca
    17941 aactcatcaa aattgtattt tattctgaaa tattttaatg ctgggataag aacacattat
    18001 tcaattcaga taacttgccc gatctggaac aactaaatta tatctattat cttcatcagt
    18061 tgccagtttc ccacgtttcc cgttttcctg aataagcctt ttaacaaagc cagacaaaca
    18121 gttcatcccg ggtgatactt tgatattgcc ctgtgtgaga tcgtggctta aaactcatta
    18181 aaatgcgtca gttttatata tgtaactttc tgcatataaa aaaagcagcg ggaacaggat
    18241 tgcccgctgc cgaattcaac cacaaacgtg ttgagcatta tacggtgtcg tgccggtatg
    18301 gtagccggaa gatcacgacg ttattgcccc gagaatggcc cccggaacaa tttcatcatc
    18361 ctgccggacc tgatcagcag aatttattac ttcaatctga atacgtttta attctgttgc
    18421 aagttcaggg aagtcttccc ctcggaagat cccttcttta atagctttta atagccgtat
    18481 ttttaaatct ttatccagga caatcatttt cttatacttt gcaatagttc attaacaatc
    18541 tgttcaatag tttcttcatt aagttgttca aactgaacgc tggcatcaat ggttgataac
    18601 ttcggaaggc agtattgaag caatttctca ataatgttcc accggtcccg gggatccagc
    18661 cgcatcaaat caacttccag ttgctgacgg ttatcattca caattttctg aagccaggcc
    18721 ctcgtttcgg tagtgacttt attttttact cccttcggcc tgcccttcgg attcccggac
    18781 tgtccgggtt taaattgtga atgaaccatg actatctgtt tttatcctgt tcttttcaga
    18841 tctttttccc gcctattccc ctgtctctcg cgtttaacag ttctgcagct gcttttccat
    18901 aagcttgctg cgctgcaatg tatgatgaat ccgggatccc gatttctgat aaaagagatt
    18961 gaaatatatt gaacatctca tcaatatttt tgattgcata agctgcccga aatgctgcta
    19021 cgtaatctct cgaaagttta atttcatcca tgttattaat tattaaattt attcctcttc
    19081 gggtgaaaca aaatatactt ttgtcgcgtc taaatgattt gacataaaag ctttttgcat
    19141 cgtgattgca aaaggttcct ttggaaaggg gttcattcct tccaggactg ccagtttgcc
    19201 ccgcttcccc ttcgccttct catcgaatgt tttcatcctg ggccaaattg ttgatttact
    19261 ttttttcttc atgattcatc aattttgata atgtcgtgcg gattgactga aaatttaatc
    19321 cagccgcctg tcggctcaaa acgtgctgca atagcatcgt agtaaaatag acggctgatc
    19381 ccgtcagggt tcacttcccc ggttgccaga atgtcgttta atgcctggca taattttttc
    19441 acctttgcca gcttcttttt ttcttctggg gtagttgcat aatgccgata actatttttc
    19501 agcttttcaa cttctgcgga caggatattg atttttcctt ctttcagttc aatggtgttc
    19561 caatagatga caggctttgc ccctggtacg aaagtcggca tatcctcaaa tttttctgcc
    19621 agccttaaaa gttctgacgt ttccagatcc gggatattgt caaaggttac aatcaaatcc
    19681 ggatatttgg cctgcattct tgcaagctgc ccggcccgga tcatattatt tatttcattc
    19741 tggagcttag cttttgatcc gagggttccg gtatgtttaa agatctgttt cagctgtgtc
    19801 attgtaatat cccagcctgt tactttgcgg aattcatcaa tgaaggcctg aaaatgtttt
    19861 ttgctgaatt ctgacatttc atccaggacc cggattcttt ccctgtcaat taaaaacaat
    19921 tccggttcat ctgtggcaac tgtttcaacg gcctggaata ttgtgtcagt aattgttttc
    19981 gttccgagtt tctccgcaac tgcctgcagg tttttctcat ccttctggct ggccctcaaa
    20041 tcaatacgtt tatttctgcc cgtcataatg aactgttttg taattatttt cgaatgtacg
    20101 taatttctat ctgtttgcaa atagtttgaa aattagttat taacaataaa ataatatttt
    20161 tataaaaaac attgaatcaa taatgtaaac aaagaatata agaataaagc cctctgttga
    20221 taagccagaa gatatattta taaggggctt gtaaggacct ttaaaaaact tataaggacc
    20281 ttcaaagaaa agggccctgt tgacctgggt aatgcaaatc ccgccacagg gccccggtac
    20341 actgttttat aggtgtacac ttgatgtaca ttaaaaaaat ggtaatgaat ctgatatttc
    20401 aaattctctc ttgtaacaat ccataacttc aagccggctg ccatgtttta atacagtcat
    20461 tataatatta ctatcttcaa ccgtgatttg tatcttttct tgtacaaagt caacgccatt
    20521 atctgtaaca atatcaagcc gcttgcatat ctcaatcact ctgttgaaaa gtttatcata
    20581 ataatattcc cctataatac ctcggtatct gaattcagga tcaccgtcac gctcagagta
    20641 acaaatatta taattaggtt ctgttttcac atgagcacat tcaccattct taaaattata
    20701 agtgtaaata atttcaaact cttcaatttc aaaggaaagt tcagccgtga atattaaatc
    20761 cgggttatcc gcgcttaatt gacaaattat ctcttttggt gtatgaagac aactttcgat
    20821 atagacggat ccatcttcat caattacact cataagatac agttcttcgt caaaaggttc
    20881 tttggcaata atatccctga atctttcagg atcactgcac catagttttg ttaaataatt
    20941 actcatcatg atttttttaa ttaatttatt aataaacagt tatttataaa gcttgcagca
    21001 tcatagcagc ggcattgttt tttattcata aaagagcccc ggaagtggca gttatgcaag
    21061 actgtatttc cggggcttat gcagtcagca cacactacta aaactgaccg cattttccag
    21121 agatagacgt ttttaccttt aatgaatatc aatgagttac agagattctg acaacataag
    21181 ggtaagagat atgtaataat acagtaaaat tgaaaaatga ttagtatttc tatgccgagg
    21241 tcaattttca tgatttttga ttctgatttt tggacaatag cctgttactt ctttaatgac
    21301 ttcagccatt atttttttta cttttccagc ttcccttaca atgagtatat ccgagggcaa
    21361 aatagaatca tgttttgtta tgaatggcac acctggaaga tcctgcacaa ttcgtggtgc
    21421 tactctttga aggatcacaa aagattctat tttctgcaat aggattgcaa gttttctgta
    21481 atcttctttt tttatcgttt tgaacagctg ccatacattc gggaagcatt tagaaaacag
    21541 acgggccgct ttactatatc tttccgctcc attgcggcca aagaaaacaa tgaacaactg
    21601 ttctttcagg tccttccggt caataaatga aatgtcattc tctttgaatt tattcatcat
    21661 gtattgataa aatttcccgc ttgttacg
//
LOCUS       DAOSPX010000015        21514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2639_length_21514_cov_4.904283, whole genome shotgun sequence.
ACCESSION   DAOSPX010000015 DAOSPX010000000
VERSION     DAOSPX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21514
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2639_length_21514_cov_4.904283"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLZ75_01575"
     CDS             complement(<1..451)
                     /locus_tag="PLZ75_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOS71119.1"
                     /translation="MKPDLVNRIFSISQQTDFQAITLEIFKLQSEKNRVYRDFIGYLG
                     IDPAAVKRPEDIPFLPAGFFRNHRVVTGSGSAKLVFGSSGTGGEMTSRHYIADPEIYE
                     MSFQGGFQLFYGDPSEYFIAALLPSYAERANSSLIYMARGLIGKSNCR"
     gene            complement(448..987)
                     /locus_tag="PLZ75_01580"
     CDS             complement(448..987)
                     /locus_tag="PLZ75_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71120.1"
                     /translation="MGNVRGKIVSILLLAAVLLISCDRKKEPERSGTATIDNELVLDK
                     ELQTYVNYGFLFSEARLASNVESPRPDITVFRDGDKISLDANNLSPSFYKYGEYNSQQ
                     EAAEAFRNLSSATVSRWEERATMLKINQIWLYRSNTERYAKIRIISIQLENRSLQEYV
                     KCTFEWVYQPDGSLNFPGK"
     gene            complement(1081..2343)
                     /locus_tag="PLZ75_01585"
     CDS             complement(1081..2343)
                     /locus_tag="PLZ75_01585"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOS71121.1"
                     /translation="MKKIQDFNFKGIRAIVRVDFNVPLDKETFEVTDDTRIRGALPTI
                     RKITGDGGSCILMSHLGRPKGRMEKFSLKPVLPVLEKHLGMKVLFADDCLGESAVKMT
                     AGLKPGEVLLLENVRFYPEEEGKPVLPDTATEEEKKAAKAELKIRQKEMAQKLAGYAD
                     VYVNDAFGTAHRAHASTAVIAGFFPGDRKMFGFLIDSELAAMDKVLKDAGRPFTAVMG
                     GAKVSDKILIIENLLDRVDNLIIGGGMTYTFIRAMGGRTGNSLCEEDKLDVARAIIEK
                     AREKGVNFLLPVDGVNADRFDAGADTYVSAIDDVRDGWMGLDIGEESIRLFEDVIVKS
                     KTVLWNGPMGVFEIDKFAAGTTAIARAVAKATEQGAFTLIGGGDSVAAINKNGLADKV
                     SYVSTGGGAMLEYMEGKILPGIAAIRGD"
     gene            2504..3001
                     /gene="tpx"
                     /locus_tag="PLZ75_01590"
     CDS             2504..3001
                     /gene="tpx"
                     /locus_tag="PLZ75_01590"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOS71122.1"
                     /translation="MAKITFHGNPVTTSGNLPRAGSQAPDFTLVKSDLDSLRLSDLKG
                     EKLILNIFPSLDTPVCATSVRKFNELAAGKPGVRVLAISKDLPFAHGRFCSTEGINNV
                     LTLSGFRDSDFGKAYGIGITDGPLAGLYARSIVVIDENGVVKYNELVPEIVQEPDYDA
                     ALNAL"
     gene            3060..4205
                     /locus_tag="PLZ75_01595"
     CDS             3060..4205
                     /locus_tag="PLZ75_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOS71123.1"
                     /translation="MNFSQIPGQKEIISKLIRSVREERVSHAQIFTGPEGCGSLAVAL
                     AFAKYISCENRNDHDSCDVCRSCVKYEKMIHPDLHFVFPVIKKGRKDSESVSDSFLAE
                     WREFAGKSPYFTINAWLDSINVENAQGLIFASEAEEIIKKLSLKTYESDYKIMIIWLP
                     EKMHPAAANKLLKLIEEPPDKTLFLLVSDEPDRIIPTILSRCQLVRLPGFTNDEIREY
                     LTGRYSAGRQRAEEIAKVVSGNIIKAIELFENEDSSRQNLDRFRKLMRFAWKRDVMSL
                     MKWSDDISVTGREAQKKFLSYSLGMLRENLMMNLGQMKNNIVFLAGEEASFSGNFHPF
                     ISLANIYPLNDEFNQAFSHVEANGNPRIIFLDLAFKVTRLISRTPKV"
     gene            4268..5419
                     /gene="ricT"
                     /locus_tag="PLZ75_01600"
     CDS             4268..5419
                     /gene="ricT"
                     /locus_tag="PLZ75_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOS71124.1"
                     /translation="MSENKELQDQAEEELLLPDTTYKPGCNKLDSFNWLRNLPECAAE
                     NEIVEIRFKNTRKGFYRNVNNLRLEIGDIVAVEASPGHDIGRVSMTGRLVREQLKELK
                     IPPSPDDLRKVYRKAKAADIQKWEEAKKLEIPTMLRSRQISGELNLNMKIGDVEYQGD
                     KTKAIFYYIADERVDFRQLIKVLAEEFRVRIEMRQIGARQEAGRIGGIGSCGRELCCS
                     THITNFNSVTTTHAKYQELSLNPQKLAGQCGKLKCCLNFEFDCYVDAQKGFPPRDIPL
                     QATEETAHFLKMEVHKKLYWYTCGPDSTSNMIAVPVERVKEVQDMNRKGRKPRRILNS
                     VTEWEGSDPSGKEDDEYSITRFDSPVQAKKKRRHSRKKKNRKSNEKKKN"
     gene            5400..5879
                     /locus_tag="PLZ75_01605"
     CDS             5400..5879
                     /locus_tag="PLZ75_01605"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOS71125.1"
                     /translation="MRRKRIKAGILLIPLMVFVAACGKNILFTDSADIPDKTWKLTNI
                     PEFRYNSADTTGKADISFTIRTGSDFPYRNIFLFVTAISPDGRSLTDTLEYDLADEKG
                     RWYGKGSGDVHELRLPWKSNVFFPLKGEYVFRIQHGMRSTDLRGVYDIGLRIERITI"
     gene            5889..6584
                     /gene="trmB"
                     /locus_tag="PLZ75_01610"
     CDS             5889..6584
                     /gene="trmB"
                     /locus_tag="PLZ75_01610"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOS71126.1"
                     /translation="MGKNKLARWAELKTFRNVTEAGPDIRSGKDHPVKGKWKQNIFHN
                     NNPLVLELGCGKGEYTTGLAERFPDRNFIGVDIKGARMWRGAKTAHEKNIPNAAFLRT
                     RIEFAGSFFAADEVDEIWLTFPDPHAGKKKTNKRLTSPWFLNLYRSFLRDNGTVYLKT
                     DNEELYNYTRSLAEKNKLPLVKATSDLYGEDNPDEIFLIRTYYENNYLDEGRKIHLLA
                     FTLPGNKISEDEI"
     gene            6574..6933
                     /locus_tag="PLZ75_01615"
     CDS             6574..6933
                     /locus_tag="PLZ75_01615"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOS71127.1"
                     /translation="MKSEKPPAENDFFGMVYEVCRLIPYGRVTSYGAIARYLGSGRSA
                     RTVGWALNACHTQPGFIPAHRVVNRQGLLTGKHHFGNSTTMEQLLANEGVYVEDDRVV
                     DFTEKFWDPSAEIDPLI"
     gene            complement(7239..9086)
                     /locus_tag="PLZ75_01620"
     CDS             complement(7239..9086)
                     /locus_tag="PLZ75_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOS71128.1"
                     /translation="MKALSIRGILFISLFFVCSPALFSQSNPRLIVRSDDMGAFHSVN
                     MACIKAYKNGIQTVVEVMPVTAWFPEAVRLLNENPGIDVGIHLAITSEWENVKWRPLT
                     HSPSLTDENGYFYPMMSPHNAYQGKSIRENNWDITEIEREFRAQIELTLRNIPQASHI
                     TGHMGALRFDPEVNELAHKLSEEYNLTFVESEELRKKYNVTQAWYDGSNATDKKESSF
                     ISMLNRLEGGKNYIFLDHPAFNNDEMESVGHVGYEWVGEDRQGVTDLLTSEKVKQTIG
                     ERGITLINYNDLTKALPRGTPESEKVSEKGIRNYMEAVKKSGQDLHSVMIVRNGKVVF
                     EHWFGDHVSNKPHIMYSVSKTYTATAIGFAVAEGRLSVTDKVISFFPEKLPANVSQEL
                     KDLEIKHLLTMSVGHDPAAVREKTRTNDDWVSVFLAEPVTEKPGAQFNYNSVATYMLS
                     AILQKVTGEKVIDYLYPRLFRPLGIVGATWEECPKGINVGGWGLYVKTEDMAKLGQLL
                     LQKGEWNGVQLLPASWIEEASSSKIESIPAGAKKEEIKIKKNDSDWLQGYGYQMWRCR
                     NNAYRADGANGQFILILPEKNTVIVTTANISDMQAELNLIWKYLYRALK"
     gene            complement(9302..9640)
                     /locus_tag="PLZ75_01625"
     CDS             complement(9302..9640)
                     /locus_tag="PLZ75_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71129.1"
                     /translation="MQSATISADVEASSSLPAGESDKLAQYIHNLFERIQDYQTSNNK
                     GAIRICLSSFNLYAEGDNKNDLERSGCRIDAIERLMTFTLVMLQQYIIIAAKPVLRYT
                     TAKQGKPNIF"
     gene            complement(9749..9967)
                     /locus_tag="PLZ75_01630"
                     /pseudo
     CDS             complement(9749..9967)
                     /locus_tag="PLZ75_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765242.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            10530..10769
                     /locus_tag="PLZ75_01635"
     CDS             10530..10769
                     /locus_tag="PLZ75_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOS71130.1"
                     /translation="MSDTLTIKERVLNALNKIRPYLQADGGDIELLEITDDMSVRVKL
                     TGACHGCPYSMMTLKAGVEKAVRQDVPEIKQVISA"
     gene            10922..13510
                     /locus_tag="PLZ75_01640"
     CDS             10922..13510
                     /locus_tag="PLZ75_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS71131.1"
                     /translation="MEKNTSTTRFYHSLTSRYNIWFNGYESFKRGVQKVSAGHRDDYA
                     ELLNVFEYSDPSTVSMCSSDMDQAIMKASKLISLKSITAKPEIKDRNKLTPAELEFYN
                     RKEFNDWVDASYLLIAKSHFYKHEFGEAESVLNYTITEANDAEVRKEASVWLARVFNE
                     TGKYPDSFRTLNETDIGNESSRYLKEMYYTTFADLNIKQKRYSEAIAPLEKAMDFVSG
                     KRTKYRLCYLLAQLNELEGNSAAATELYRKVVRMNPPYDVEFNARINIAGVFDINSGN
                     PNEIRKELEKMLRNSKNNDYHDQIYFSLAGLAMKSGNEKEALSLYRKSVTVPSSNPNQ
                     KGRSYQALGDYYYDNADFIRAGRYYDSTVFFLDQNHPEYRSLREKSADLNTLVSQLVI
                     IQQEDSLQRVAAMPESERNMFIANLINEVTKAETEGRSTDYSDRYNLGQYYENERRFQ
                     GNIAQEGKWYFYNQAALTFGRTEFRRRYGDRKLEDNWRRANKITVSSAQPGMTAAESE
                     LSGGDEAEEAVLDNKKPEFYLRNLPLTDSLLQVSNEKIALASLNAGLAYSEKIGDNQR
                     ANETLESLLRRFPSHHLVPETLYTLYKINQAGSPAVAETYRQRLLERFPETEFAKILS
                     DPEYYEKKLAREKRSEQLYNKAYNEYSEENFTAAISTIDEVLNNDDDDMLIPKFMLLR
                     AYSVARVSDERAFREELGRLIKLWPQTEESKKAGEIIEFLNEKTPELKIEEETVIARE
                     LFEADTSSSHIFALIIPDPAFNLNLATFDVISYNIDNYTNRNFRTESSLADDKYIIIS
                     VSGFSSFSEAMEYYRKFRIESVVRNSAGKGMYAFVINNFNLEVMRKDKNPERYSLFFR
                     ENYHPE"
     gene            13522..15081
                     /locus_tag="PLZ75_01645"
     CDS             13522..15081
                     /locus_tag="PLZ75_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOS71132.1"
                     /translation="MRKIRILWTDDEIESLRPHIFFLTEKGYEVDTCTNGNDTVDLVR
                     QNHYDLIFLDEHMPGLSGIETLRLIREIRTDIPVVMITKSEEEDIMEAAIGSAIEYYL
                     IKPVKPNQVLLAIKKILDQKRLVTKKATTDYRLEFSRIGNMISSASSFSDWAELYRKI
                     VFWESELEKSTDPGMSEILMMQENEANNSFSKFVMKNYLGWLDPKNENAPLMSPSLFS
                     SKILPHVSPGQSLFFILIDNMRFDQWKKISAELTGLYRIMDEDIYCSILPTATQFSRN
                     AIFAGMMPSRIVATTPQYWISDNEEEGKNEFEEELFKNQLAGKRLRISWSYHKISNSQ
                     EGRKVNEKIKQLLENDLNILVFNFVDILSHARTEIGLIRDLAGDESAYRSIVRSWFIH
                     SSLFELLNMLASHPVKIIFSTDHGTIRVQNPLKIIGDKKTSANLRYKMGRNLNYNPAE
                     VFELRDPEAAFLPRANISSRYIFARNKDYLVYQNNFNYYAGYYKDTFQHGGISMQEML
                     LPVVLLEPVIK"
     gene            15078..15491
                     /gene="tsaE"
                     /locus_tag="PLZ75_01650"
     CDS             15078..15491
                     /gene="tsaE"
                     /locus_tag="PLZ75_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOS71133.1"
                     /translation="MKITIAHIRDVPEAAGELLKISEGKKVFAFYGALGSGKTTIIKA
                     ICRELGAGDEGSSPSFTIVNEYRSNSGEAIYHIDFYRIGKQEEVFDFGLEEYLWSGAY
                     CFMEWPEIVEDMLPLNTFRIRITVEKNGERILETV"
     gene            15555..16775
                     /locus_tag="PLZ75_01655"
     CDS             15555..16775
                     /locus_tag="PLZ75_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOS71134.1"
                     /translation="MNGKSRSVKLNIGNSALMPKEEHLETTVRSRGISIGIPADVRDD
                     EKRVALTPEAVNILVESGNEVIVQKGAGAGCNYADKDYSENGAVITESPARVYGSDVV
                     IKVAQFSTKEANYLKGNQVVMSYLNVFKLSEETLASLVRKKITAIAFERIRDNSGIMP
                     VTESMSEICGVTSVMLASEYLSNHHGGKGIMLGGITGITPTEIVILGANTAGEFAARA
                     ALGLGAQVKLFDNSLQKLRRIETLVGQRLQTSVFHPQVLRKALRSADVLIGAIGLDEL
                     KSWYYVTEDMVRTMKNKAVIIDLSIDRGGCIETSEYRAMKDPVYEKHGVIHFSAWNLP
                     SRVPRTASMALSNIFAPILQNLADSGGITQVLKNDPGFRNGVYLYNGILTNEILGHKF
                     NILPKDLDLLLSAF"
     gene            complement(16814..17728)
                     /locus_tag="PLZ75_01660"
     CDS             complement(16814..17728)
                     /locus_tag="PLZ75_01660"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOS71135.1"
                     /translation="MSSSKFQPPFLRKGDEVAIISPAYFIEEEQLEDSVRILEGWGLR
                     VHISKNALKREGPFAGTERERISDIQEMTRSGNIKAIFCSRGGYGLLKIIDKIDFSAL
                     KRYPKWYCGFSDVTILLVWLSEVVGLMTIHGEMPLNYLNREIRPETLDSLHDALFGGL
                     EPVSWRGEFFRSGTATGEVTGGNLSLLYSLMGTIAEPKTRGRILFLEDTDEYLYHIDR
                     MMTSLRLAGKLRGLAALVAGGFTSVKNTKIPWDTSVEQIISSVTSTYRYPVLTGFPAG
                     HTGDNRAFYIGRRASLVVTEGEATFSYL"
     gene            complement(17752..18420)
                     /locus_tag="PLZ75_01665"
     CDS             complement(17752..18420)
                     /locus_tag="PLZ75_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HOS71136.1"
                     /translation="MSRVLNILVEGSTEKEFVSNLIYPYLYDNGISNVRTITIETSPG
                     FKGGDVRYKARYKPNIQKILKGEEDMRVTSLIDYYRLRNDFPCYEKSRTLARAEQRVE
                     LIEQGCYEDINDIRFLPYIQLHEFEGLLFSASIGFNELFPDLPKKNKRELLDIIDKFP
                     NPEMINDRPEFAPSKRLERLIPNYRKPLYGSMIALENGFPAIMDKCPRFRNWIETLIK
                     RMKE"
     gene            complement(18417..19505)
                     /locus_tag="PLZ75_01670"
     CDS             complement(18417..19505)
                     /locus_tag="PLZ75_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS71137.1"
                     /translation="MGIYSIKIQNFKSIRETDDMLIKPLNVLIGPNGAGKSNFISFFK
                     FLNRLYEQQLQLYISQNGRAENFLFFGSKGSDFLAGRIVFDNNWKNEYVFKMVPDQSG
                     NLIFAEEWSNFTSPGSASKNFSNISIGGNPESVLKNDDGYRNKYLRTQFNEFKLFHFH
                     DTSFNSKVKQPSGTQDYASLREDGGNLAAFLYRLQESHPKHFKVITRIIQSIAPFFQD
                     FYLVPDEINTQHIFLRWKEKGSDQLFTAHNLSDGTLRMICLTTLLMQPHLPGTIIIDE
                     PELGLHPFAIQKLAAMLKSASDKSQIIVSTQSVNLVDQFTADDIIVVERSDNQTVFKR
                     QSEETLAEWIEEYSLGELWSKNVLGGTP"
     gene            complement(19590..19952)
                     /locus_tag="PLZ75_01675"
     CDS             complement(19590..19952)
                     /locus_tag="PLZ75_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOS71138.1"
                     /translation="MSIKTAFISRTQKIKIMPESNTLGQILRGHRESKGLLLRQVAAA
                     MEMDTALLSKFERDERKPNKEQVFAFAKYYNVNADELLLAWLSDKIAVEVQNEDLAKE
                     ALKAAGKKVELFKRKKKP"
     gene            20359..>21514
                     /gene="metG"
                     /locus_tag="PLZ75_01680"
     CDS             20359..>21514
                     /gene="metG"
                     /locus_tag="PLZ75_01680"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOS71139.1"
                     /translation="MMKKYKRHLITSALPYANGPLHIGHLAGVYVPSDIYTRFLRLRD
                     EDVISICGSDEHGVPITLKARSEGVTPQEIVDRYHAINKKAFEDFGIAFDIYSRTSNK
                     IHYETASEFFLTLYNKGIFTEKTTEQYYDEEAGTFLADRYIRGTCPHCGNEDAYGDQC
                     EGCGSSLSPTELINARSMISGGKPVLKETLHWYLPLDKYEQWLKEWILEGHREWKTNV
                     YGQCKSWLDQGLQPRAVSRDLDWGIPVPVENAEGKVLYVWFDAPIGYISATRELTPDW
                     EKYWKDSDTRMIHFIGKDNIVFHCIIFPVMLKAEGSYILPDNVPANEFLNLENDKIST
                     SRNWAVWLHEYLADFPGKQDSLRYSLCASAPETKDNDFTWKEFQARNNNEL"
BASE COUNT         6180 a         5001 c         4640 g         5693 t
ORIGIN      
        1 accgacagtt acttttccct ataaggccgc gggccatgta aatcagtgat gaattggctc
       61 tttcggcata ggatggcaga agagcggcaa taaaatattc tgagggatcc ccgtaaaaaa
      121 gctgaaagcc tccctggaaa ctcatttcat aaatctccgg atcagcaatg taatgccggc
      181 tggtcatctc cccgccggta ccgctgctcc cgaaaaccag ctttgcgctt ccggatccgg
      241 tgaccaccct gtgattcctg aaaaatcctg cgggaaggaa gggaatatcc tccggccgtt
      301 tcacagcagc aggatcaata ccgagatatc cgataaaatc cctgtaaacc cggttttttt
      361 ctgactgcag cttaaatatt tcgagggtta tagcctgaaa atccgtttgt tgtgagatgc
      421 tgaaaattct atttaccaga tccggcttca tttgccggga aagtttaaag atccgtcagg
      481 ctggtaaacc cactcaaagg tgcatttgac atattcctgc aaagacctgt tttccagctg
      541 gatggaaatt attctgattt ttgcatagcg ctcggtgttt gaacgataca gccagatctg
      601 gtttattttc agcatggttg cacgctcttc ccagcggctt acggttgcag atgagagatt
      661 tctgaaagcc tctgctgctt cctgctgact gttgtattcc ccgtatttgt aaaacgaagg
      721 acttaaattg tttgcatcga gtgatatttt gtccccatcc ctgaaaactg tgatgtcagg
      781 cctgggactt tcaacatttg atgcaagcct tgcctcggaa aacaggaatc cgtaatttac
      841 ataggtctga agttccttgt ctaaaaccag ttcattgtca atagtggcag tcccgctgcg
      901 ttccggttct tttttcctgt cacaggatat aaggagcact gcagccagca gcaatattga
      961 tacaattttt cccctgacat ttcccataac aacaaagata aggcatttcc cgcagatccg
     1021 ggatccgttt ccggtcggcc cttacggtca ccctttaccg ttccggccgg tcctgccggt
     1081 tcaatcaccc ctgatggcag cgattcccgg cagtatcttc ccttccatgt attcaagcat
     1141 tgcaccgcct ccagtagaaa cgtagctcac cttgtcggcc aggccgtttt tgtttattgc
     1201 agcaacggaa tctcctccgc caatcagggt gaaggcaccc tgttccgttg cctttgcaac
     1261 tgccctggca atggctgttg ttccggcagc aaacttatct atttcaaata cgcccatagg
     1321 tccgttccag agaacggttt tcgatttcac tatcacatct tcaaaaagcc tgatgctttc
     1381 ctctccgatg tccagcccca tccatccgtc ccgtacgtca tcaatggcag acacataggt
     1441 atcggctccg gcatcaaacc tgtcggcatt gacaccgtcg accggcagca ggaaattcac
     1501 acccttttcc ctggctttct caattattgc ccttgccaca tccagcttgt cctcttcaca
     1561 caaggaattg cccgtcctgc ctcccatcgc cctgatgaag gtatatgtca ttccccctcc
     1621 tatgatcagg ttgtcaaccc tgtcgagaag gttttcaatg atcagtatct tgtcggaaac
     1681 ctttgcacct cccataaccg cggtgaaggg tctgcctgca tccttcagca ccttgtccat
     1741 agcagctagt tcactgtcaa tcaggaatcc gaacatctta cggtcgcccg ggaaaaaacc
     1801 ggcaatgacg gcagtactgg catgtgccct gtgtgcagtt ccgaatgcat cattgacata
     1861 aacatcggca tagccggcaa gtttttgagc catctctttc tgcctgatct tcagttctgc
     1921 ctttgctgct ttcttttctt cctcagtggc tgtatcagga agaacgggct ttccttcctc
     1981 ctcggggtag aaacggacat tctcaagcag aagcacttcc cccggcttca gtccggcagt
     2041 cattttaacc gccgactcac ccaggcagtc atctgcaaac agaaccttca ttccaagatg
     2101 cttttcaaga accggcaata ccggtttcag ggaaaatttc tccatccgtc ccttcggtct
     2161 gcccagatgt gacatcagaa tgcatgagcc cccgtctccc gttatcttcc ttatggtagg
     2221 caaggcaccc cttatccttg tatcatcagt tacttcaaat gtttccttgt cgagaggcac
     2281 attgaaatca acccgtacaa ttgccctgat gcctttaaaa ttgaaatcct gtatcttttt
     2341 catatctgtg ttttttaggt tttatcccgg aatttttaaa gcatacaaac ctaatatttt
     2401 tttatcaatt acagacatta accggttaaa agtagatgaa caattcattc gctgataatg
     2461 ttaactgcat gtaaaagttt aattaatttt gaagaaaaaa gctatggcaa aaattacttt
     2521 tcatggaaat ccggtcacca catccggcaa tcttccccgg gcgggaagcc aggcacctga
     2581 tttcacactc gtaaaatcag atcttgacag tctcagactt tccgatctga agggagaaaa
     2641 gcttatactg aacatttttc ccagtcttga caccccggtt tgtgcaacct cggtgagaaa
     2701 attcaatgaa ctggctgccg gaaaacccgg cgtcagggta ctggccatct ccaaggatct
     2761 gccttttgcg cacggtcgct tctgttcaac ggaaggaata aataatgtat taactttatc
     2821 gggcttccgc gacagtgatt tcgggaaagc atacggaatc ggaattacag acggccctct
     2881 cgcgggatta tatgccagaa gcattgtggt catcgacgag aacggagtgg tgaaatacaa
     2941 tgaactggtg cctgaaattg ttcaggaacc cgattatgat gcggcactca atgccctgta
     3001 gagaggaaat cagtaagtaa agccccaagc cgggacaaac aggcagaaat gaaatcagga
     3061 tgaatttttc acagataccg ggccagaaag aaataatttc aaaactgatc cgctccgtcc
     3121 gggaagaaag ggtcagtcat gcacagatat tcaccggtcc ggaaggctgc ggctccctgg
     3181 ctgtggcact ggcattcgca aagtatatca gctgtgagaa caggaatgat catgattcat
     3241 gtgacgtatg ccgctcatgc gtgaaatatg aaaaaatgat acatcctgat cttcattttg
     3301 tttttccggt aataaaaaag gggaggaagg attccgaatc cgtaagtgat tcatttcttg
     3361 ctgagtggag ggagtttgcc ggcaaatcac cgtatttcac aataaacgcg tggcttgatt
     3421 caataaatgt ggaaaatgcc cagggactga tatttgcatc cgaggcggag gagatcatta
     3481 agaaattaag tcttaagact tatgaatccg actataagat aatgatcatc tggcttcccg
     3541 agaaaatgca cccggcggca gccaacaagc ttctgaaact cattgaggaa ccgcccgaca
     3601 agactctgtt tctccttgtt tcggatgagc ccgacagaat cattcccact atcctttcac
     3661 ggtgccagct cgtacgtttg ccgggattca caaatgacga aatcagggaa tacctgaccg
     3721 gcagatactc tgccggcagg caaagagcgg aagaaatagc gaaggttgtc agcggaaaca
     3781 ttataaaggc aatcgaatta tttgaaaacg aagattcatc caggcagaat cttgaccgtt
     3841 tcagaaagct gatgcgtttt gcctggaaac gcgatgtcat gtcccttatg aagtggtctg
     3901 acgatatttc cgtaaccggc agggaggcac agaaaaaatt cctttcatac tcactgggta
     3961 tgctgaggga aaacctgatg atgaatctcg gccagatgaa aaacaatatt gttttccttg
     4021 ccggagagga ggcgtccttt tcagggaatt tccatccctt tatcagcctt gcaaatattt
     4081 atcccctgaa tgatgaattc aaccaggcat tcagtcatgt tgaggcaaac gggaatcccc
     4141 gcattatttt tcttgatctt gccttcaaag tgaccaggct catatcaaga acaccaaaag
     4201 tataattgat attaattcat ttattgacta cttttgcgct ttgaaccgac aatctgttga
     4261 tcatcatatg tcagaaaata aagaattaca ggatcaggca gaagaagagc ttctgctgcc
     4321 tgataccaca tataagcccg gatgcaataa gcttgattcc ttcaactggc tgagaaacct
     4381 gccggaatgt gccgctgaga atgaaatagt tgaaataagg ttcaagaata cccggaaggg
     4441 cttttacagg aatgtcaaca acctgcggct tgaaatcggg gatattgttg cggttgaagc
     4501 atctcccgga catgatatcg gaagggtatc aatgaccgga aggctggtaa gggaacagct
     4561 caaggaactg aagatcccgc cgtccccgga tgatctcagg aaagtatacc ggaaggccaa
     4621 ggcagctgat atccagaaat gggaagaggc gaaaaagctt gagattccta ccatgctgcg
     4681 gtcaaggcag atctcggggg aactcaatct caacatgaaa atcggagatg tcgaatacca
     4741 gggtgataaa accaaggcaa tattctatta tattgctgat gaaagagttg acttccggca
     4801 actgataaag gtgctggcgg aggagttcag ggtcaggata gaaatgcggc agataggtgc
     4861 caggcaggaa gccggacgaa taggaggcat aggatcatgc ggaagggaac tgtgctgttc
     4921 aacccatatc acaaacttca actctgtcac cacaacccat gctaaatacc aggaattatc
     4981 acttaacccc cagaagcttg caggacagtg cggaaagctc aagtgctgcc tgaattttga
     5041 atttgactgc tatgtggatg cccagaaagg atttcctccc cgggatatac cccttcaggc
     5101 aactgaggaa acggcccatt tcctgaaaat ggaagtccat aagaaactat actggtatac
     5161 atgcggtcct gattcaacat caaacatgat agccgtgccc gttgaaagag tgaaggaagt
     5221 acaggatatg aacaggaagg gccggaaacc acggcgcatc ctcaacagcg tcacggaatg
     5281 ggaaggatcc gacccgtccg ggaaagagga tgatgagtac agtataaccc gctttgattc
     5341 ccctgtacag gcgaaaaaga aacgccgtca ttcccggaaa aagaaaaaca ggaaatccaa
     5401 tgagaagaaa aagaattaaa gcgggtatat tactgatccc tttaatggtc tttgttgctg
     5461 cctgcggaaa aaatatcctt ttcaccgatt cagccgacat tccggacaag acatggaagc
     5521 tgacaaacat tcccgaattc aggtataact ctgccgatac cacgggcaaa gccgatattt
     5581 cattcacaat ccgcaccggc tcggattttc cctacaggaa catattcctg tttgtcacgg
     5641 caatatcccc cgatggcaga agccttaccg acacccttga atatgatctc gccgatgaaa
     5701 aaggcaggtg gtacggaaaa gggtccggag atgttcacga actcaggctg ccctggaaat
     5761 caaatgtatt tttcccgctt aaaggtgaat acgttttcag aatacagcac ggaatgcgca
     5821 gtaccgatct caggggagta tatgatatcg gactgagaat tgaaagaatc actatctgac
     5881 tatctggggt gggaaagaat aaacttgccc gctgggcgga gctgaagact ttcaggaatg
     5941 taacagaggc cggtccggac atccggtcag gaaaagatca tccggtaaag ggaaaatgga
     6001 aacaaaacat atttcataac aataatccgt tagtccttga gcttggctgc ggaaagggag
     6061 aatatacaac aggcctggcg gaaagatttc ccgacaggaa ttttatcggg gtagatatca
     6121 agggagccag gatgtggcgc ggagcaaaga ccgcacatga aaaaaacatc ccgaatgctg
     6181 ctttcctgag aacaagaatc gaatttgcag gatctttctt cgccgctgat gaggttgatg
     6241 aaatatggct tacctttcct gacccgcatg ccggaaagaa aaaaacaaat aaaaggctca
     6301 catctccctg gttcctcaac ctttaccggt ccttcctcag ggacaatggt actgtctacc
     6361 tgaaaaccga taacgaagag ctgtataact ataccagatc gcttgccgaa aagaataagc
     6421 tccccctggt gaaggcaaca tccgatctgt acggggaaga taaccctgat gaaattttcc
     6481 ttatcagaac gtattacgag aataattatc tggatgaagg gcggaagatc cacctgctgg
     6541 ctttcactct cccgggtaac aaaatcagtg aagatgaaat ctgaaaaacc accggcagaa
     6601 aatgattttt tcggtatggt gtatgaggtg tgcaggctca tcccctacgg cagagtaact
     6661 tcctacggcg ctatcgccag gtatctcggc tcgggcagat cggcaagaac tgttggctgg
     6721 gctcttaatg cctgccatac acagccgggc ttcattccgg cacacagagt cgtaaaccgc
     6781 cagggccttc ttaccgggaa acatcatttc ggcaattcca caacgatgga acagctcctt
     6841 gcaaatgagg gtgtctatgt tgaagacgac agggttgttg atttcactga gaaattctgg
     6901 gacccttctg cagaaattga tccgctgatt taatctctga tcctgtccgg cagcggccgg
     6961 gagcttgcgg ttgctttata tatcaatttg tcagctttgg gtcttcctcc tttacggtaa
     7021 gttaactgat tttccccttc ggatcggttt tccggtttat cccgtagagt gttgcaatgc
     7081 ttgccaccga aaacaccaca caaaaaattc tgcataaagc caaattctgg aaactgcacg
     7141 aacatactcc cataaactaa cgacaacgtt ttatgctcaa catactcctt gacggatttg
     7201 aaggtaaatt gcagactctt ctgaccgcac tatgtctgtt acttcaacgc ccgatacaga
     7261 tatttccaga tcagattgag ctctgcctgc atgtcactaa tattcgctgt tgtaactata
     7321 actgtatttt tctcaggaag gatcagtata aactgcccgt tcgccccatc agcccgataa
     7381 gcattattac ggcaacgcca catctgataa ccatagccct gaagccagtc gctatcattc
     7441 ttctttattt taatctcctc ctttttagct ccggcaggga ttgattctat cttggaggaa
     7501 gaggcctcct ctatccatga tgcgggaagc agttgtaccc cgttccattc tcctttctgt
     7561 aacaacaact gccccagctt tgccatatct tcagttttaa catacagacc ccagccaccg
     7621 acatttatcc ctttaggaca ctcttcccag gtggctccaa ctattcccaa cggacggaat
     7681 aaacggggat ataaatagtc aatcactttc tcacctgtca ctttttgtaa aatggcagag
     7741 agcatatagg ttgccacact gttataattg aattgtgctc cgggtttctc cgtaacaggc
     7801 tctgccagga aaacggagac ccaatcgtca tttgtacggg tcttctccct gaccgctgcc
     7861 ggatcatgcc cgacagacat ggttaacaga tgctttatct ccagatcttt aagttcctga
     7921 cttacatttg caggcagctt ttccggaaag aatgagatca ccttgtctgt cacgctgagt
     7981 cgtccttccg ctacagcaaa cccgatggct gttgcggtat atgtcttact taccgagtac
     8041 attatgtgtg gtttatttga aacatgatcg ccaaaccaat gttcgaacac aactttcccg
     8101 ttcctgacaa tcataacact gtgtaagtcc tgcccgcttt ttttgacagc ctccatataa
     8161 ttcctgatac ctttctcaga taccttttcc gactcgggtg taccgcgggg aagcgctttg
     8221 gtcagatcgt tatagttgat aagtgttata cctctctctc cgatcgtttg cttaaccttt
     8281 tcacttgtca acaggtcagt taccccctga cgatcttcgc caacccactc gtaacctaca
     8341 tgacctacac tctccatctc atcgttatta aatgcaggat gatcaagaaa tatgtaattc
     8401 tttccccctt ccagcctatt gagcatactg atgaaactgc tctctttctt gtcagtagca
     8461 ttagaaccgt cataccatgc ctgagtgaca ttgtatttct ttcttagctc ctcactttcc
     8521 acgaatgtca gattgtactc ctccgacagt ttgtgagcca gctcattcac ttcaggatcg
     8581 aatcgcaatg ctcccatgtg accggtaatg tgacttgcct gtggaatatt cctcaaggtc
     8641 aactctatct gagcccggaa ctctctttct atttcagtga tatcccaatt gttctcacgg
     8701 atagatttcc cctgataggc attatgcgga ctcatcatcg ggtagaagta accattttca
     8761 tcagtcaggc tcggactgtg ggtcagcggg cgccatttta cattctccca ttcactggtt
     8821 atagcaagat gtattccgac atcaatgccc ggattctcat tcagtaagcg tactgcctca
     8881 ggaaaccatg cagtaaccgg cattacctct acaacagtct gaatgccatt cttatatgct
     8941 ttgatacatg ccatgttcac agagtggaat gcacccatgt catctgaacg gacaattaac
     9001 cgtggattac tctgactgaa aagggcaggg gagcatacga aaaataagga tataaatagt
     9061 attcctctga ttgataatgc tttcatatta ttctaattgt tgttttgtat gtttaatgtt
     9121 ttatcggtgc ttctcgttta tactcatatt attatacttc ctgcatgctg taagtcaaaa
     9181 catacagaca atcattctgg ttatcagcag caggatatca attgcagaag atattcaaag
     9241 aatccttccc tgattactat gcccaataat agtgcaaaac caaaactcaa gagagtgccg
     9301 attaaaatat atttggtttt ccctgttttg ctgtcgtata cctcaggacc ggtttggctg
     9361 ctattataat gtattgctgc aacatcacta atgtaaatgt cattaaacgt tctattgcat
     9421 ctatccggca gccggatctc tcaagatcat ttttattatc tccctctgca tacagattaa
     9481 atgacgagag gcagatcctt atcgctcctt tattattgct tgtctgataa tcctgtattc
     9541 tctcaaagag attatgtata tattgggcca gcttgtcaga ctcccccgca ggtaaagagg
     9601 aagatgcctc cacatcagct gatattgttg cactttgcat tacgactact tatgttatgt
     9661 aaatataaaa cttactttta cacccaatgc tgcggcaatc ttattgatgg tggaaagtgt
     9721 caggttaaat tttccgtttt caatcttgga tatttgtatt gtccagatat actttatcaa
     9781 ccttccctaa tccccggata ccactttttt caccacgtaa ccggctaata agcccggcct
     9841 tttccatata cgaaaaataa tcatccaagg agttccggct tacaccaatt atttctgata
     9901 ttttggagaa atttggttta aaaggcacac ttgctgcgat aatggaaagt aatcgtttca
     9961 aatttctggt aaagtgttct cattgctttg atgtacaatt ttccacaaag ataaaaaacg
    10021 cacaatgtat tcaacaatat ttctaatatc tgttcatttg aatatgcact taactatctg
    10081 tccaaaacca cagtggcaaa agatatggaa ctactatact cgtatattat ctgttcacat
    10141 tctttgccat caaactccag cctgtaacat atccggtgtc ccttatgggt ttttgcctct
    10201 tcccaataag agacaatttc atgttgctcc atatctccga ataatgtagg gaaagaaagt
    10261 ttgtaaataa tcacatctgc cggtaaaaaa tctatagaag tcatcttggg ataaaattcc
    10321 ataaacaaac catctacccc gaaataactg ccgtattccc cgatctgtaa gctatatccg
    10381 ccaacatcag aagtaatacc aggtttaaag gtttcagaag aaactgaagc accgggtatc
    10441 cattccggaa agattttgcg ctaaaagaaa aaagtagtaa tattgcgatt tatttataat
    10501 taatttaaat aaaaataaga acatcatcca tgtcagatac attaaccata aaagaacgag
    10561 tgctgaatgc acttaacaag atcagaccgt atcttcaggc agacggcggg gatattgaac
    10621 tgctggaaat aacggacgac atgtcggtca gggtaaaact gacaggtgca tgccatggct
    10681 gtccctacag catgatgaca ctcaaggcag gtgttgaaaa ggcagtcaga caggatgtac
    10741 cggaaataaa acaggtaata tccgcctgaa ggaaacagca tgccaatttt cattaccttt
    10801 gaaatactat atactgaaaa aacaagttta tagtattaaa ggtcagctat tgtcttcatt
    10861 gaaagcagca tataaatttc tgccggcttc agcacttttg tttatcctgg cagcctgctc
    10921 cgtggaaaag aacacgagta caacaaggtt ttaccacagc cttacatcaa gatataatat
    10981 ctggttcaac ggatatgaaa gcttcaaaag aggtgtccag aaagtatccg ccggacacag
    11041 ggacgactat gccgaactgc tgaatgtttt tgaatacagc gatccctcaa cggtttccat
    11101 gtgttcttcc gacatggatc aggcaataat gaaggcttca aagctgatct ccctcaagtc
    11161 aataactgca aagccggaaa taaaggacag gaacaaactg acgccggcag aactggaatt
    11221 ctataacagg aaggagttta atgactgggt tgatgccagc tatcttctta tagcgaaatc
    11281 acatttttac aaacacgaat tcggcgaggc tgaatctgtt cttaactata ccatcacgga
    11341 ggcaaatgat gctgaggtaa ggaaagaggc atccgtctgg cttgcaaggg tcttcaatga
    11401 aacggggaaa tatcctgact ccttccgtac cctgaatgaa acggatatcg gaaacgaatc
    11461 atcacggtat ctgaaggaga tgtattacac cacatttgca gatctcaata tcaaacagaa
    11521 gcgatattcc gaagcgatag cccctctcga gaaggccatg gattttgttt ccggcaaaag
    11581 aacaaaatac cgcctctgct atctccttgc ccagctcaat gagctggaag gcaattccgc
    11641 agcagctaca gaactgtacc ggaaggtggt cagaatgaat cctccctatg acgttgaatt
    11701 caatgcaagg attaatattg caggcgtttt tgatataaat tccggtaatc ctaatgagat
    11761 caggaaagag cttgaaaaaa tgctcaggaa ctcaaaaaac aacgattacc acgatcagat
    11821 atatttctcg cttgccggac tggcaatgaa atcggggaac gaaaaggaag ctctcagcct
    11881 gtacagaaaa tcagttacgg taccttcatc caatccgaat cagaaaggaa ggtcctatca
    11941 ggcccttggt gactattact atgacaatgc cgatttcatc agagccggca ggtattatga
    12001 cagcaccgta ttttttctag atcagaatca tccggaatac cggtcactga gagaaaaatc
    12061 agcggatctg aacacactcg tttcacaact ggtaataatc cagcaggagg acagtctgca
    12121 aagagttgcc gcaatgccgg aatccgaaag aaatatgttc atagccaatc tgatcaacga
    12181 ggttacaaaa gctgaaaccg agggaagaag cacagattac agcgacagat acaaccttgg
    12241 ccagtactat gagaacgaaa ggcgattcca gggaaatatt gcacaggaag gcaaatggta
    12301 tttttacaac caggctgccc ttactttcgg gagaactgaa ttcaggcgcc gttacggcga
    12361 caggaaactg gaagacaact ggaggagggc aaacaaaata actgtttcat cagcacagcc
    12421 gggaatgact gcggccgaat cagaactcag cggcggagac gaagctgagg aggctgttct
    12481 tgataacaag aaaccggagt tttatctccg taacctgccg ctcaccgatt cactcctcca
    12541 ggtatcaaat gaaaagatag cattagcctc acttaatgca ggactggcct attcagaaaa
    12601 aatcggggac aaccagaggg caaatgaaac tctggaaagc ctgctcaggc gctttccctc
    12661 ccatcatctt gttccggaaa cactttacac tctgtataag ataaaccagg caggcagtcc
    12721 cgctgttgcc gagacatacc ggcagagact gcttgagaga ttccctgaaa cggaatttgc
    12781 aaagatcctg tccgatccgg aatactacga gaaaaagctt gcccgggaaa aaaggtccga
    12841 acagctttac aacaaggctt acaatgaata ttccgaggaa aacttcactg ccgccatttc
    12901 caccatcgat gaagttctga acaacgatga cgacgacatg ctgattccca aattcatgct
    12961 cctcagagcc tattcagtgg caagggtctc cgatgaacgt gccttcaggg aggaacttgg
    13021 cagactgata aaactctggc cacagacgga ggaaagcaaa aaagcagggg aaataattga
    13081 attcctcaat gaaaagacac ccgaactgaa aatcgaggaa gaaaccgtca tagccaggga
    13141 actcttcgag gccgacacct cttcctccca tatttttgcg ctgatcatac ccgatccggc
    13201 gtttaactta aatctggcca cattcgatgt tatcagctat aacatcgata attatacaaa
    13261 cagaaatttc agaaccgaaa gcagcctggc ggatgacaag tacattatca tcagtgtaag
    13321 cgggttcagc agcttttcgg aagcaatgga atattacagg aaattccgga tcgaatcagt
    13381 tgtcaggaat tcagcaggga agggaatgta tgcatttgtg ataaacaact tcaacctgga
    13441 ggttatgcgc aaggacaaga atcctgaacg atacagtctt ttcttcaggg aaaattatca
    13501 tccggaataa gtcaaaagtc aatgagaaag atcaggatac tctggaccga tgatgagatt
    13561 gaatctctca ggccgcatat ttttttcctc acggaaaagg gttacgaggt ggatacctgc
    13621 acgaacggga atgataccgt cgacctggtg aggcagaatc actatgatct cattttcctc
    13681 gacgaacaca tgccgggact cagcggtatt gaaactctcc gtctgataag ggaaatacgc
    13741 accgacatac ccgttgtgat gataacaaag agtgaggagg aagatatcat ggaggcagcc
    13801 atcgggtccg caattgaata ttatcttata aaacccgtaa aacccaatca ggttctgctg
    13861 gccataaaaa agatactcga ccagaaacgg ctggtaacaa aaaaggccac aacagattac
    13921 agactggaat tcagcaggat aggcaatatg atatcttccg cttccagttt ttcagactgg
    13981 gcggaactgt accgcaagat agtattctgg gagagcgaac ttgagaaatc cacggacccg
    14041 ggaatgtcgg aaatactgat gatgcaggag aacgaggcaa acaactcatt cagcaagttc
    14101 gtaatgaaga attatctcgg atggcttgat ccgaagaatg aaaacgctcc cctcatgtcc
    14161 ccgtccctgt tcagctcgaa gatactgccg catgtttcac ccggacagtc cctgttcttc
    14221 attctcatcg acaatatgag gtttgatcag tggaaaaaaa tatccgccga actgaccgga
    14281 ctctaccgga taatggatga agacatatac tgcagcatcc tgcccactgc cacacagttc
    14341 agcagaaatg ccatatttgc cggcatgatg ccctcgcgta tcgtcgccac aacgccacag
    14401 tactggatca gtgataatga ggaggagggc aagaatgagt ttgaagagga gcttttcaaa
    14461 aaccagcttg caggcaagag actcaggatc agctggtcat atcacaaaat aagcaacagc
    14521 caggaaggca ggaaagtcaa cgaaaaaata aaacagttgc tggaaaatga tctcaacata
    14581 ctggtcttca attttgttga tatactttcc catgcacgta cggagatagg actgatcagg
    14641 gatcttgccg gtgatgaatc agcctacagg agcattgtac gctcatggtt cattcattcc
    14701 tccctctttg aactgctcaa catgcttgca tcccatccgg taaaaataat tttcagcacg
    14761 gaccacggga ccatcagggt tcagaatccc ctgaaaataa ttggtgataa aaaaacatca
    14821 gccaacctgc gatacaagat gggcagaaat ctcaactaca atcccgcgga ggtctttgaa
    14881 ttacgtgatc cggaagccgc tttccttccc agggcaaata tatccagcag atatattttt
    14941 gcccggaaca aggactatct tgtttatcag aacaatttca actactatgc cgggtattac
    15001 aaggatacct ttcagcacgg gggtatttca atgcaggaaa tgcttctccc tgttgtactt
    15061 ctggagcccg tcatcaaatg aaaataacca tagcccatat cagggatgtt ccggaagcag
    15121 ccggagagct gctgaagatt tctgaaggga aaaaggtatt tgctttctat ggtgccctgg
    15181 ggtccggcaa aacaacaatc ataaaggcca tctgccgtga actgggagca ggcgatgaag
    15241 gaagcagtcc ctctttcaca atagtgaatg aatacaggag caactcggga gaggcaatct
    15301 atcatatcga cttctacagg atagggaagc aggaggaggt tttcgatttc ggactggagg
    15361 aatacctctg gtcaggcgca tactgcttta tggagtggcc tgaaattgtg gaagatatgc
    15421 ttccgcttaa tactttcagg ataaggatca ccgtggaaaa gaacggggaa agaattcttg
    15481 aaacggtctg aacatttctg ctggtacaga aattcaattt ttattacctt tgaaacaaca
    15541 acgatatccc cggaatgaac ggaaaaagca ggagcgtgaa actaaatatc gggaacagtg
    15601 ccctgatgcc caaggaggag catctggaga ccactgtgag atccagggga atttccatag
    15661 gaattcccgc tgacgtaaga gatgacgaaa aaagagtggc cctcactcct gaagcagtta
    15721 acatcctggt cgagtcaggc aatgaggtga ttgtccagaa aggggccggg gccggatgca
    15781 actatgccga caaggactac agtgaaaacg gggctgtgat aacagaatcc ccggcacggg
    15841 tgtacggttc cgatgttgtt atcaaggttg cacaattctc tacgaaggaa gcaaattacc
    15901 tgaaaggcaa ccaggtggtc atgtcgtacc tgaatgtatt caaactgagc gaagaaacac
    15961 ttgccagcct ggtcaggaag aaaataaccg ccattgcttt tgaaaggata cgcgacaaca
    16021 gcggaatcat gccggtaacc gaatccatga gtgagatctg cggtgtcaca tcggttatgc
    16081 tggcatcgga atatctcagc aatcaccacg gagggaaggg cattatgctg ggcggaataa
    16141 caggcattac tcccacggaa attgtcatcc tgggtgcaaa taccgccggg gaatttgctg
    16201 caagggccgc gctgggcctg ggtgcacagg taaagttatt cgacaattca cttcagaaac
    16261 tgagaaggat tgaaaccctt gttggccaga ggctgcagac ctcagtgttt catccccagg
    16321 tcctccggaa ggccctccgg tcagccgatg tgcttatcgg ggcaatcggg cttgatgaac
    16381 tcaaatcatg gtactacgtg acggaagaca tggtaaggac aatgaagaac aaggctgtca
    16441 tcatcgacct gagcatagac cgcggaggct gtatcgaaac atccgaatac cgggcaatga
    16501 aggacccggt gtatgaaaaa catggtgtca ttcacttttc cgcatggaac ctgccttcaa
    16561 gggtgcccag gactgcctca atggcattaa gcaacatttt tgcacccata ctgcagaatc
    16621 ttgcagattc aggaggaata acacaggttc tcaaaaatga tccgggattc agaaacggcg
    16681 tttacctgta caacggtatt cttacaaacg aaatactcgg acataaattc aatatactgc
    16741 caaaggatct ggatcttctc ctttcggcat tctgagtccg gcctgattct gccggcatgc
    16801 tgcatactga tcatcagaga taggaaaatg tggcttcgcc ttcagtgaca accagacttg
    16861 ccctgcgtcc tatgtagaat gcccggttat caccggtatg gccggccgga aaacctgtaa
    16921 gaacaggata tctgtaagtt gaggtcacag acgagatgat ctgctccacg cttgtatccc
    16981 aggggatttt tgtgttcttc accgaagtaa aaccacctgc cacaagggct gccagtcccc
    17041 tgagctttcc ggcaagcctg agggatgtca tcatcctgtc gatatgatac agatattcat
    17101 ccgtatcttc caggaaaagg atcctgcccc ttgtttttgg ttcggcaatt gtacccatca
    17161 ggctgtacag caatgaaaga ttgcctcccg tcacctcacc cgtcgctgtg ccggatctga
    17221 aaaactctcc ccgccagctc accggctcaa gtccgccgaa cagggcatca tgcagtgaat
    17281 ccagggtttc cggtcttatt tccctgttaa ggtaattcag gggcatttcc ccgtgtatgg
    17341 tcatcagtcc caccacctca ctcagccaga caagcagtat tgtaacatca ctgaagccgc
    17401 agtaccattt cggataacgc ttcagtgccg aaaaatcgat tttatcaatg atcttaagca
    17461 agccatagcc tcccctggaa cagaatattg ccttgatatt tccggaccgg gtcatttcct
    17521 gaatatcgct gattcgctcc ctttcggttc ctgcgaaagg accctcacgc tttagagcat
    17581 tttttgaaat atgaaccctc aggccccagc cctcaagtat cctcacagaa tcctcaagct
    17641 gttcctcttc gatgaaatat gcaggtgaaa tgatggctac ctcatcaccc ttcctcaaaa
    17701 acgggggctg gaacttagag cttgacatac ttcaatattt tttgtagaat ttcattcttt
    17761 catacgctta atcaatgttt caatccagtt tctgaatcgt ggacatttgt ccataattgc
    17821 agggaaacca ttttctaaag caatcatact tccatacaaa ggtttcctgt aatttggaat
    17881 gagcctttcc agtctttttg aaggagcaaa ttccggacgg tcgtttatca tttcaggatt
    17941 tggaaacttg tcaattatat ctaacaattc ccttttattt ttttttggca agtctgggaa
    18001 aagttcattg aacccaatgg aagcagaaaa caataagcct tcaaattcat gaagttgtat
    18061 gtagggtaaa aatcttatgt cattgatatc ttcgtaacaa ccttgctcaa ttaattcaac
    18121 cctctgttca gccctggcaa gtgttctgct tttttcatag catggaaaat cgtttcgcaa
    18181 gcggtaatag tcaattaagg aagttacccg catatcctcc tcccctttca atattttttg
    18241 aatattaggc ttatatcttg ccttatagcg tacatcacca cccttaaagc ccggacttgt
    18301 ctcaatagtg atggttctta cattgctgat gccattatca tacaaataag gataaatgag
    18361 attggaaaca aactcttttt ccgttgaacc ttcaactaaa atatttaaca ctctgctcat
    18421 ggtgttcctc ccaatacatt cttgctccac aattcaccca atgaatattc ctctatccat
    18481 tcagcaagtg tttcttcgct ttgccgtttg aaaactgttt gattatcact tctttcaacc
    18541 acaataatat catcagcagt aaactgatcc accagattga cagattgcgt tgaaactata
    18601 atttgagatt tgtccgatgc gcttttcagc attgctgcta atttctgtat ggcaaaagga
    18661 tgcaagccca attcgggttc gtcaattata attgtgcctg gcaaatgggg ttgcatcaac
    18721 agggtagtaa gacaaatcat ccttagcgtt ccatccgata agttatgtgc tgtaaacaat
    18781 tgatcggaac ctttttcttt ccagcgtaaa aaaatatgtt gtgtattaat ctcgtcgggt
    18841 accagataaa agtcctgaaa aaatggagct atggattgta taatcctggt gattaccttg
    18901 aaatgcttag gatgcgattc ctgaagcctg tataaaaaag cagccaggtt gccaccatcc
    18961 tctcgtaaac ttgcatagtc ttgagtacca cttggttgtt ttactttaga gttaaaactg
    19021 gtatcgtgga agtgaaatag cttgaattcg ttgaactgag tcctaagata tttatttctg
    19081 taaccatcat cattttttaa aacactctca ggattaccac caatactaat attggaaaaa
    19141 ttttttgacg ctgagcccgg acttgtaaaa ttgctccatt cttccgcaaa aatcagattg
    19201 ccggattggt caggaaccat tttaaagaca tattcatttt tccaattgtt atcaaaaaca
    19261 attctccctg caagaaaatc tgaacctttg cttccgaaga aaagaaagtt ttcagcgcgt
    19321 ccgttttgac tgatatataa ttggagttgc tgttcataaa gcctgttcaa aaacttaaag
    19381 aagctgatga aattactttt acctgctcca ttaggcccta ttaatacatt caggggtttt
    19441 atcagcatat catctgtttc cctgatgctc ttgaaatttt gtattttaat ggaatatatg
    19501 cccatgttga tttgattatg acgttttaaa atccagccct gcttctttca tctgcgacac
    19561 cgtgttggtt ttcattttta atggaattat taaggttttt tcttcctttt aaaaagttca
    19621 accttttttc cggcagcttt caaagcttct tttgcaaggt cttcattttg cacttctacg
    19681 gctatcttgt cacttagcca ggccaaaagc aattcatcag catttacatt gtaatacttt
    19741 gcaaatgcaa acacctgttc tttgtttggt ttcctttcgt cccgttcaaa tttgctcaac
    19801 aatgccgtgt ccatttccat tgccgctgcc acttgtcgta ataacaaacc ctttgattca
    19861 cgatgtcctc ttaatatttg acctaatgta ttactctcgg gcattatttt tattttttgt
    19921 gttcgtgaaa taaatgcagt tttaatagac ataaatttga cttgtcaata attgtctaaa
    19981 attacaacta tttttaaaat gcaggaggga ataatccgct ctttttgatt ttacttgtga
    20041 atttggtttg caggaaaggt acagggatct tgttcattcc ataaaagtcc ttgcaaggca
    20101 ccgttcatga acggaaagat ctgaacacag ccggtacttc tcagctataa aaatacttgg
    20161 atccgaaagc cgcagagttt tgatccatta catcattgta tcatttttta ttatctttgg
    20221 cctttttcct gaacaccatc aacacaacag atttatcaaa cccgcacatg actgattatc
    20281 attacaaaca tccggcaaat ccaatttacg gtcatgtatc tttaatccgc ctgtacgggg
    20341 attactaaaa aaattttact gatgaaaaaa tataagagac accttattac atccgcattg
    20401 ccgtatgcca atggcccgct tcatatcggc catcttgccg gagtatatgt tccttccgat
    20461 atttatacaa gattcctcag gctcagggat gaggatgtga tctcaatctg cggttcggat
    20521 gagcatggtg ttcccatcac tctcaaggcc agaagcgagg gtgttactcc ccaggagata
    20581 gtcgacaggt atcatgcaat aaacaagaag gcatttgagg atttcgggat tgcatttgat
    20641 atttattccc gtacctccaa caagattcac tatgaaacag cttccgaatt tttccttaca
    20701 ctgtacaaca agggcatatt caccgaaaaa accaccgaac agtactatga tgaggaagca
    20761 ggtacattcc ttgcagaccg ctatataagg ggaacctgtc cgcattgcgg taatgaggat
    20821 gcctacggcg accagtgtga aggctgcggg tcttccctca gcccgacaga gcttataaac
    20881 gctcgttcta tgatcagtgg cggcaagccg gttctgaagg aaacccttca ctggtacctt
    20941 ccgctggata aatatgaaca atggctgaag gaatggatac tggaaggtca cagggaatgg
    21001 aagacaaacg tatatggcca gtgcaagtca tggctcgacc agggcctgca gccaagggcg
    21061 gtaagccgcg atctggactg gggcattccc gtgccggtgg agaatgcgga ggggaaagtg
    21121 ctgtatgtct ggttcgatgc cccgatagga tatatatccg ctacccggga actgacaccc
    21181 gactgggaga aatactggaa ggacagtgac acgaggatga ttcatttcat cggaaaggac
    21241 aatattgttt ttcactgcat catctttccg gtaatgctga aagccgaggg aagctatatc
    21301 cttcccgaca atgtcccggc aaacgaattc ctgaatcttg agaatgacaa gatatccacc
    21361 tcacgcaact gggctgtatg gcttcatgaa tacctggccg acttccccgg caagcaggat
    21421 tccctgcggt attcactctg tgcaagcgcc ccggaaacca aggataacga ttttacatgg
    21481 aaggaattcc aggcccgcaa caataatgaa ctgg
//
LOCUS       DAOSPX010000016        20939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2724_length_20939_cov_5.131967, whole genome shotgun sequence.
ACCESSION   DAOSPX010000016 DAOSPX010000000
VERSION     DAOSPX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20939
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2724_length_20939_cov_5.131967"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLZ75_01685"
     CDS             complement(<1..301)
                     /locus_tag="PLZ75_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71140.1"
                     /translation="MKIKLFIVALILFGGSAVLALAQDKAAGQSQEVPVPAGRGVYVD
                     ADNNGICDYFEMRGANYGFGRGQGYGRAQAAVQGYGRGQAAAQGYGRAQMAVPGGG"
     gene            451..909
                     /locus_tag="PLZ75_01690"
     CDS             451..909
                     /locus_tag="PLZ75_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOS71141.1"
                     /translation="MPNRRRHRRLTNPPRMKGYKPFGIPMRELESVILLYEEFEALRL
                     ADYENLLHAEAAERMNISRPTFTRLYDKARKSVARAFVEGKAIMIQGGTFIAEDYWYK
                     CEECHETMVSDHPVDHCRECDSDNLTTLNEAAAKEQKDVKAGKTVKPDNF"
     gene            1048..1386
                     /locus_tag="PLZ75_01695"
     CDS             1048..1386
                     /locus_tag="PLZ75_01695"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOS71142.1"
                     /translation="MPAGDRTGPLGQGPGTGRTLGFCYGYDSPGFMKGRGMRMGRGSG
                     FIGGGARGPGFRGGAGRGRGFGRFMPGYPWAPSLSREDEVRMLKSEAEALKRSQQEIE
                     RRLGELEKEA"
     gene            complement(1443..2411)
                     /locus_tag="PLZ75_01700"
     CDS             complement(1443..2411)
                     /locus_tag="PLZ75_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS71143.1"
                     /translation="MISFGPVPSRRLGKSLGINNIIAPKTCSYACVYCQAGRTHIKSI
                     KRDSFFEPEKIYRSVNEHISLLPSDNYPDYLTLVSNGEPTLDINLGRTIELLRRTGIP
                     VAVITNASLLFSDTVREDLYRADWISLKVDAADDDLWKKINRPDRNLDFDRHMKSIFL
                     FSAEYKGVLCTESMIIDGLNDFPDHFEKLAGIVKSINPEKAYLSVPTRPPSEKWIKPP
                     DAEKLNAAWQIFSKRKIKTELLTGFEGTDTGSGGNIYEDILNITAVHPLREDSMLKLL
                     EKNNADFGIVESLINQKLIRPVVYMDKRFFIREYHFSRNKSGDSNT"
     gene            complement(2442..3299)
                     /locus_tag="PLZ75_01705"
     CDS             complement(2442..3299)
                     /locus_tag="PLZ75_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOS71144.1"
                     /translation="MASDYRCFYCLARSVENLLEKIPMPAEKKKSFTLDMIDLYLERQ
                     RKELTPDFSRDLYRILRGYSGIDDPYKEEKHRSNLQALELLPKLEEIIRQSSDPFVTA
                     IRLAIAGNVIDFAVHRNFNLEETIEKSLKAPFAADHTAELKSAVEKASSILYLGDNAG
                     EIVFDKLFIRTMNHSNVTFAVRGMPVINDATLEDARFTGMHEVARVISNGYDAPSTLV
                     EKSGSEFREYFRNADLIISKGQGNLEGLYKLNDKRIFFILLVKCNVIAELLGVEKNSL
                     VTFNSSYLK"
     gene            3632..5098
                     /locus_tag="PLZ75_01710"
     CDS             3632..5098
                     /locus_tag="PLZ75_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOS71145.1"
                     /translation="MANNLFRTKSPQQMMEAASGSEHGLKRALTATNLVFVGIGCIVG
                     TGIFVITGTAAAQYAGPALSISFLISAVVCMFVALCYTEFASMIPISGSAYSYAYMTV
                     GEILAWFIGWNLVLEYIFGASLVAVGWSGYVVSFLKDFGIVIPPELSSAPFTYHGGWI
                     KTGAILNFPSVFIVGLITTVLVFGIKESVRFNNIIVVIKVAVILLFIGFGLFYINPAN
                     YSPFIPENTGEPGHFGWSGIFRAAGIVFLAYLGFDAVSTVAQESKNPQRDMPIGILGS
                     LAIVTVLYISVSLVLTGMVNYKFLNVPDPIAVGINAAGSKLFWLRPIIKIGAIAGMSS
                     VILVLLTGMPRILYIMSVDGLLPPVIGRIHRKFRTPHITTIIVGSIVALISGLFPIDL
                     IGELVSMGTLLAFSMVCISIIILRIKRPDMHRPFKTPLVYFVASAGAAGCLYLMTSLP
                     HSTWVRLGGWTVIGMLIYFLYGRKHSKLNEIPGKSGGS"
     gene            complement(5128..6177)
                     /locus_tag="PLZ75_01715"
     CDS             complement(5128..6177)
                     /locus_tag="PLZ75_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71146.1"
                     /translation="MLYDLDPEWDSFEIRDVRFSNSMVSESLKEEGFEVCIEELNDYH
                     LEKILRHYDPADTIVFNLCETLPGIECSERKVVEIIEGRGFTCTGNTAGVIELSYDKL
                     RVKECLASLGIKVPYGAVLAPEEAGGWNLFPAIVKPSREHCSLTITEKSVVYDTASLR
                     EQILYVNNLLKQPAMVEDFIDGREFHVSVWNNEIPEMLPPAEMDFSAFSEARERLCTY
                     DSKFRPGSLHYEKIETLIPAPLTERQYEKLEKTVLKTWREFGCRDYARFDFRLRDNDF
                     FLLDINPNNDISADTSFALAAEMKNYSYGRMVKRIAMMAAGRHSLLAGEIQELVWAGN
                     GSGNGCPRGPGHLKS"
     gene            6336..7625
                     /locus_tag="PLZ75_01720"
     CDS             6336..7625
                     /locus_tag="PLZ75_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS71147.1"
                     /translation="MRVKGTPARGLFGTTTGFFFGFAAVALYGPTAVEFREAMDLSPA
                     LVGLLIAIPSLSGSLLRIPFGAWVDISGGRKPFLILMIMSAAGLGGVTLLLSTSYPDG
                     MKGLYWLVLLLGFLSGCGIATFSVGIAQTSYWFPHSRQGIALGTYAGLGNLAPGIFSI
                     VLPLYLTRFGFISAYFAWFLFLITGIILYAAIAQNSYYFQFKKAGFTVNEAKTRAQEL
                     GQELFPSGGMKESLVSSAKITNTWALVVLYFTTFGGFIALTAWFPTYWNEYQHFDPVT
                     AGLLTAVFSLLASLIRVFGGKIADKSGGEKTILVSMSVILLSALFLSFLSDVFTSVVM
                     VILLAAGMGISNAAVFKLVPAYVPKAVGGASGWIGGLGAFGGFALPPVMGAIAGKYGT
                     AGYARGFLVFVMLSVINLLIIYFLIRKHRTETVHRPG"
     gene            complement(7664..8269)
                     /gene="pncA"
                     /locus_tag="PLZ75_01725"
     CDS             complement(7664..8269)
                     /gene="pncA"
                     /locus_tag="PLZ75_01725"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOS71148.1"
                     /translation="MKTLLIIDVQNDFIPGGSLEVPLADRVVPLINSIQHRFDLVLAT
                     QDWHPEDHISFASGHSGKKAFDTIDLEGNEQTLWPDHCVQGSSGAMFHPDLETDRIAA
                     IFRKGMNPAVDSYSGFYDNMHLISTGLSGYLREKGATDLYFCGIAGDICVYHSINDAI
                     EEGFRATLIEDASAPLNREVFEVQKKELQKKGVRIINSNEI"
     gene            complement(8271..9677)
                     /locus_tag="PLZ75_01730"
     CDS             complement(8271..9677)
                     /locus_tag="PLZ75_01730"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOS71149.1"
                     /translation="MFNYTATYTDHYQLTMAQAYFLNGQKENISVFDYFFRKLPFNSG
                     YAVFAGLENLLDILQELKFGRNDLEFLRSNGFHPEFLKYLEQFRFRGNIYSSREGDLV
                     FPVRPVLQVEADIIEAQLVETILLNVLNFQTLVATKASRMRKAAGNDTLIDFGLRRAQ
                     GPGGYYASRAAYIGGFDATSNVLAGRDYGIPVSGTMAHSYIQSIDDELAAFNHFSDVH
                     PDNSVLLVDTYDTLNSGIPNAIKTARRLEEKGHRLLGIRLDSGDLAYLAKKSRKMLDE
                     AGLDYVKIAVSNQLDEYIIKSLKAQKAPIDVFGVGTSLVTGFPDAALDGVYKMAYSDG
                     KPRIKISENVTKITLPHRKQVLRAFDSDGRCTGADVVVLNSEKDTDIMYHPLYPHKSF
                     PLKNKKKEALLLKVMEGGKRTSESVSLKETAQYSSERLALLPDEYKRFDNPHAYKVGL
                     SEKLMKERNRLIEDHKKY"
     gene            complement(10027..10965)
                     /locus_tag="PLZ75_01735"
     CDS             complement(10027..10965)
                     /locus_tag="PLZ75_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOS71150.1"
                     /translation="MQTILGANGTIGKVLAGELTRYTDRIRLVSRNPVRINETDELLA
                     LDLTKPGAVEKAVSGSEVVYLVIGFEYSTKVWELSWPALMRDTIDACLKHNAKLVFFD
                     NVYMYDKEYLAELTEDTPVNPSSRKGVVRKWIAGMLLEAVKTKQLKALIARSADFYGP
                     DNQNSFVTQMILKNLAAGKKALWFIGPGYKHSFTYTPDAAKATALLGNTPDAYGQVWH
                     LPTDSRPVTGKQFISMIASEFGIPDPKLSFLPKWMLYPLGLFIPVMKEMPEMMYQYDR
                     DYFFDSSKFTGRFNLKATPYEEGIKNTCKAWKQTGE"
     gene            complement(11209..11739)
                     /locus_tag="PLZ75_01740"
     CDS             complement(11209..11739)
                     /locus_tag="PLZ75_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71151.1"
                     /translation="MKKILLTGMLFLYISGILIGQSIYSGSFDLKNQSAFNLISENEI
                     VTVQIAKKQIDINNSEKNTYGLRKIKSNQFLFFCGSDSIQIVQAKKKLTFQPGLVFDY
                     KKRTLKELILVDAEGKIALNAEYDFKYPVANYKINIQGNTKIKELLSYATYYLFDKSK
                     KLKNAYETPYIYFGPY"
     gene            complement(11965..12384)
                     /locus_tag="PLZ75_01745"
     CDS             complement(11965..12384)
                     /locus_tag="PLZ75_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71152.1"
                     /translation="MKALTKNLIFFMIFFFIGTIMFRYSLSYFIENRTYDLVWIIAVI
                     YFFYNFFIGWIFGKKDYETLPLYDIGFRFHFATYLLFNIVSILWFLFDFHSHYESIRS
                     VYVTALFWGIGIIIHLVIYLITRKNAILGIEKSEIFD"
     gene            complement(12387..12758)
                     /locus_tag="PLZ75_01750"
     CDS             complement(12387..12758)
                     /locus_tag="PLZ75_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOS71153.1"
                     /translation="MNKTNYLSELLSTWEETYKKGQLTLWIFLSLKDGKKYVDEIKCF
                     IEEQSNQTMTCESQSLYRTLRKFQHLGVVDFETGEGNKGPERKYYFLTEIGHRLLENF
                     IERNISLFFNKHIKNLITKED"
     gene            13185..13358
                     /locus_tag="PLZ75_01755"
     CDS             13185..13358
                     /locus_tag="PLZ75_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71154.1"
                     /translation="MRYSRSLFGKITHVPADDIYATNANRKWAPVSISPQTSSVKDEQ
                     ASTNSRYTWPMVH"
     gene            complement(13582..15069)
                     /locus_tag="PLZ75_01760"
     CDS             complement(13582..15069)
                     /locus_tag="PLZ75_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71155.1"
                     /translation="MSEKLSRLDFLKTSGLAASGFVLGKTAVAQGRGPEILPSGKLSG
                     RTGKVNLALIGIANQGAGVARQFINSGLANIVAVCDVDLDAKGCQETIKLVPKAKQFR
                     DFRFMFDKMAGDIDAVQVAIPDFAHFPVCMAAMQLGKSVFVEKPMTRTFLEAEILARM
                     AENNPKIVTQVGNQGHSGENYFQFRAWKDAGIIRDVTAVTAFMNGSRRWHPYDPNISR
                     YPEAQPLPPGMTETDWDVWLTTAAYHDFNEKYHPGNWRGWYDFGLGALGDWGAHILDT
                     IHEFLDLGLPYEIEPIHVKNLNDYFFPLETTLRFRFPSRGDMPPCDITWYDGVDNLPP
                     FPEGYDSVSATVDSTIPPPGGVSRQERAKQAGGQPRRPQAGKIIYSKELTFKGGTHSA
                     PLFIIPEAKAKEMAPKLPPVPESPSDHYVNFLKACMGEEKTRSPFQVFAPLSQIFSLG
                     IIAVRRNKKLLFNRETKEFINDKFANSLLTDRPPRKGWESYYNIS"
     gene            complement(15343..16716)
                     /locus_tag="PLZ75_01765"
     CDS             complement(15343..16716)
                     /locus_tag="PLZ75_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71156.1"
                     /translation="MDITEKLTYRKIFLFWGPLALTWLMMAFEQPFLIAFIARLNEAK
                     FNLAAFGIAGSFAMIIESPIIMLMSASTALVRGRHSYRKLKRFTDLLNAGITVIQLII
                     LIPPVFSFIVTGLMGMPEDIARPAHTALFIFLPWAASIGYRRFYQGILIRNNLTRRVT
                     YGTVVRLVVIMIMGVILYSAGVKGAYVGAGAMSLAVFLEAIATRMMVSRTLKTLLQID
                     DTENRNLRLRSVARFYFPLALTSILSLGVQPFVTFFLGRSRMAVESLAVLPVVSSLVF
                     IFRSMGLSYQEVNIALIGKEKQNYRLLKNYAIFLGLVVTVLISVLAFTPLANLWFINI
                     SGLSQDLADMSYLPLKIMILVPALTVLLNFQRSSLIINGTTRPISVATAIELGTIIMT
                     LLVCTVFLNFIGVVAAAIAFMTGRIMSNLYLIQKQVSAVREWNIKELVTETVRLDSDV
                     HDHRLRR"
     gene            complement(16792..17898)
                     /gene="tsaA"
                     /locus_tag="PLZ75_01770"
     CDS             complement(16792..17898)
                     /gene="tsaA"
                     /locus_tag="PLZ75_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOS71157.1"
                     /translation="MKTYTVRPVGLVRNGFDNPADYNTIKEHPSSVIVHDEYSEALLN
                     IDECEYLDIVFWFHKSEQGTLSGKTHSGAVRGVFASRSPRRPNLIGITTVKLIERKNN
                     ELIVEGLDAINNTPVIDIKNCDTSLFASVTESNPVHNSILKSDPRIEIRNNIAKGETD
                     ILLIKAAQMHGHFCPGLAMGVMAAVHAMRELQADSDGMENLLAITETNNCFSDGVQFV
                     TGCSFGNNSLIYKDLGKTAFTLAGRDGKGIRICSRHESRDVIEEAFPDFRKYYRSVIA
                     EQKRDPDMVAAYKKSALERAFGTLDLPFGSLFTVKRVHTAIPDYAGIDESVVCRLCNE
                     SVMRSRTTEEGDSFTCFSCSGRDHAILDGNGIHL"
     gene            complement(17918..18676)
                     /locus_tag="PLZ75_01775"
     CDS             complement(17918..18676)
                     /locus_tag="PLZ75_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS71158.1"
                     /translation="MKITIQDIEFSYNGFPALENVNNRIEKGDFVALVGPNGSGKSTL
                     IKCMNGILKVSKGTVLIDDRAISMFSPNELAREIAYVPQSENRKAVLKVFDIVLLGRK
                     PYINWKPAEHDLRVTSEILNTLNLDHLAMRDFNKLSGGQQQIVYIARALAQEPDILLL
                     DEPIANLDIRHQIEVLNLLKKLSARGITIVIALHDINMAVRYASGIMMLRDGKVFASG
                     GREIITKQNIENLYDIRVKIIRNDNDTFILPEGL"
     gene            complement(18799..19869)
                     /locus_tag="PLZ75_01780"
     CDS             complement(18799..19869)
                     /locus_tag="PLZ75_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOS71159.1"
                     /translation="MSEQVLSQYYNFRKKRILFILFLLLLLFIIIIYSLFSGSADIGL
                     RDIYSLITGTGQAVSRQIIYNIRLPRIAGAFLAGMALSVSGAVIQSILRNPLASPFTL
                     GISGASAFGAAFAIIVLGAGSSYAGSNDMAIINNPYVVTVSAFLWSLCSVAVIIVLSK
                     YKGATPEIIILSGIIISSLFGAGISAMQYFSDNIQLASIVFWTFGDIGRASWNNCLII
                     AVVVSLVMVFFISQSWNYKALQSGDEYARSLGVNVSRVRLTGLVVASLVTAVVVSFYG
                     IIAFVGLVVPHIVRRIIGGDEQFLIPASAIGGGTFLLFSDTLARTIISPVILPVGILT
                     SFAGAPLFLFLLIKGMGKDYWT"
     gene            complement(19869..>20939)
                     /locus_tag="PLZ75_01785"
     CDS             complement(19869..>20939)
                     /locus_tag="PLZ75_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS71160.1"
                     /translation="RHIAAGSRLSSDSRGQPAGNNGSVAVRDILGREVFIPSRINRVA
                     GLRAGALRLLVYMDLADRIAGVEQNEKRGSTPYMKAHPELAQLPSLGPSMGGDAELIL
                     KSKPDLIFLTYTTAGDADALQQKTGIPVIALECTEFGTARDTLFASLKLIGKIMDRSG
                     RADTLINYIENSIAELNRRSSGTAESEKPLVYIGGVGYSGSYGINSTQPFFPPFMFLN
                     ARSPASEIDKRLISHVKGTHIDTEQLMLWNPDILFIDESGLNLVKQDLRRGTALFNRL
                     KAVEENRMYTLLPYNNYATNYEMVLANAWFAGKILYPEKFSDIDADEKTKEVYRIFLG
                     RDISDEIKPSSSAFRNISKNEL"
BASE COUNT         5553 a         4713 c         4660 g         6013 t
ORIGIN      
        1 gtccgccgcc cggcactgcc atttgtgccc tgccgtagcc ctgggctgct gcctggcctc
       61 tgccataacc ctggactgct gcctgagccc tgccatatcc ctgtcccctg ccaaaaccat
      121 aatttgcgcc gcgcatttca aaataatcac aaattccatt attgtccgcg tcgacataaa
      181 ccccccttcc ggccggaaca ggaacttcct gagactgtcc ggccgctttg tcctgggcaa
      241 gtgccaggac agctgatccg ccaaataaaa ttaaggcaac gataaaaagt ttgattttca
      301 taatttcgta cctcttttat attttatatt tatctgataa taaagtgaaa taacattcta
      361 cctgtaaaat taagaataat aaatgaacat atgtccattt aaaaattaaa tttgtaaaaa
      421 tattcatcag aagacatcca ttttttatca atgccaaaca gaagaaggca ccggcggctc
      481 accaatcctc cgagaatgaa aggctacaag cctttcggga ttccgatgcg ggaacttgaa
      541 tccgtgattc ttttatatga agaatttgaa gctttgcggc ttgctgatta cgaaaacctg
      601 ttacacgcgg aagctgcgga aagaatgaat atttcgcggc cgacttttac aagattatat
      661 gacaaggccc gtaaaagtgt tgccagggct tttgttgaag gaaaggccat aatgatccag
      721 ggaggcacct ttattgccga agattactgg tataaatgcg aagaatgcca tgaaaccatg
      781 gtcagcgatc acccggtgga tcattgcagg gaatgtgatt ctgataatct gaccaccctc
      841 aatgaagctg ccgcaaaaga acagaaggat gtaaaagccg gaaaaaccgt gaaaccggat
      901 aatttctgaa aacccggcag acactgatcc tgcataactc ttacggataa aatcagtaaa
      961 aaatcatatg aaatgaacat atgttcattt taattattat atttgtctta tgataagatt
     1021 agttatttac cataaaaaca agtaagcatg ccggcaggag atcgtaccgg accactggga
     1081 cagggtcccg gaaccggaag aacattaggt ttttgttatg gatatgacag tcccggtttt
     1141 atgaagggac ggggaatgag aatgggccgc ggatccggct tcataggcgg aggggcccgg
     1201 gggcccggct tcaggggcgg ggcaggacgg ggccgtggat ttggccgctt tatgcccgga
     1261 tacccatggg ctccttcatt gagcagggaa gatgaggtaa gaatgctgaa atcagaagca
     1321 gaggccctta aacgttcaca gcaggagatt gaaagacggc tcggagaact ggaaaaggaa
     1381 gcctgacaca ctttcctttg cccggaaaac agctgagaaa aaacagcggg gacaggatat
     1441 catcaggtat tactatcccc gcttttgttt cggctaaagt ggtattccct tataaagaac
     1501 cttttatcca tatacactac cggccttatc agtttctgat taatcagtga ttcaacaatt
     1561 ccgaaatcag cattgttctt ctccagaagt ttcagcatgc tgtcttccct cagcggatga
     1621 actgctgtta tgttcaggat gtcttcatat atgtttccgc ctgaaccggt atcggtcccc
     1681 tcaaaacctg tcagcagttc cgttttgatt ttccttttgc tgaatatctg ccatgcagcg
     1741 ttcagctttt ccgcatcagg cggttttatc catttttccg aaggaggcct tgtgggtacc
     1801 gacagatagg ctttttccgg atttatactc ttaacgattc cggcaagctt ttcaaaatga
     1861 tcaggaaaat cattcagccc gtcaattatc attgattctg tacagaggac acccttgtat
     1921 tcagcagaaa aaaggaaaat gctcttcata tgcctgtcga aatcaagatt cctgtcgggg
     1981 cggttgattt ttttccataa atcatcatct gcagcatcca cctttaatga aatccagtct
     2041 gcccggtaaa gatcttccct caccgtatcg gaaaaaagca gggaggcatt ggtaattaca
     2101 gcgacaggga tgcctgttct gcggagtagc tctatggtcc tgccgagatt aatgtcgaga
     2161 gtcggctccc cgtttgaaac aagtgtcaga taatcaggat aattatcaga tggcaaaaga
     2221 ctgatatgct catttacgga cctgtatatc ttttcaggtt caaagaaact atctctttta
     2281 atgcttttta tgtgtgtccg gcctgcctga cagtaaacac aggcatatga acatgttttt
     2341 ggtgcgatta tattgtttat gcccaggctt tttccaagcc gcctcgatgg tacaggaccg
     2401 aaacttatca tgatctccgc ggatgttgga ttgttatacg gttatttcag atatgaggaa
     2461 ttgaatgtca ccaggctgtt cttttcaacc ccgagcagtt ctgcaatcac attgcatttg
     2521 accagaagaa tgaagaagat tcttttgtca ttcagtttgt acaatccttc aagatttccc
     2581 tggcctttgg aaattatcag atccgcattg cggaaatatt ccctgaactc gctgcctgat
     2641 ttttcaacca gagttgaggg agcatcatat ccgtttgaaa tgaccctggc tacttcatgc
     2701 attccggtaa aacgggcatc ctcaagggtg gcatcattga ttacgggcat tccccttaca
     2761 gcaaaggtca cattactgtg gttcattgtt ctgataaaga gcttgtcaaa gactatttcg
     2821 cccgcgttgt cccccaggta gagaatggaa gaggcctttt ccacggcgga tttcagttct
     2881 gcggtatgat ctgccgcaaa aggagccttg agggatttct caattgtctc ctcaagatta
     2941 aaattccggt gcacggcaaa atcaatcaca ttgccggcaa ttgccaggcg tatggccgtg
     3001 acaaagggat cagatgactg cctgatgatc tcctccagtt ttggaagcag ctcaagagcc
     3061 tgaagattgc tcctgtgttt ttcctctttg tagggatcgt ctatgcccga atatcccctg
     3121 agtatcctgt aaagatcccg cgaaaaatcg ggagtaagtt ccttacgctg ccgttcaagg
     3181 taaagatcaa tcatatcgag ggtaaagctc ttcttctttt ctgccggcat gggtatcttc
     3241 tccagcagat tttcaacgga acgcgcaaga cagtaaaagc aacggtagtc agaggccatg
     3301 ggaataaatg tttttggcgg taaagtaaat gaacataggt tcattttgca aataattacc
     3361 gtcaattaat taaactatgc caataaagta atattctgtc attttctcca tggttttgct
     3421 tcggataatt aacaattcac cctgaaatgc cggacaagat atttctttta aatgataaga
     3481 atggattatt tccattattt tagcccactg tttctgttcc ggacatattt ccggcatttt
     3541 tttccggcaa tcttttcctg caggaatccg ggctaatctg cccgggcagg ctcctggaac
     3601 agaatcagaa caggtttatt tacaccggat tatggcaaac aatcttttca gaacgaagtc
     3661 tccccagcag atgatggagg ctgcatcggg tagcgagcat gggctaaaac gtgccttaac
     3721 agcaacaaat cttgtttttg taggtatcgg atgcattgtc ggtacgggta ttttcgtaat
     3781 aacaggaacg gcagcggccc agtatgcggg acctgcactg agtatttcct tcctgatttc
     3841 agctgtcgta tgcatgtttg tagctctatg ctatacggaa ttcgcatcga tgataccaat
     3901 atccgggagc gcttattcat atgcatatat gactgtagga gagatactcg catggtttat
     3961 cggttggaat ctggtgcttg aatatatttt cggggcatct ctggtggcag tcggatggtc
     4021 ggggtatgtt gtcagtttcc tgaaggattt tggtattgtg attccgccgg aactcagcag
     4081 tgctcccttt acataccacg gtggctggat aaaaaccggg gccattctta attttccctc
     4141 agtgtttatt gttggcctta tcacgaccgt gcttgttttt ggtataaaag aatcagtgag
     4201 attcaataat attattgttg ttatcaaagt tgcagtgatc ctccttttca taggatttgg
     4261 gcttttctac atcaatccgg caaattacag ccccttcatt cctgaaaata ccggtgaacc
     4321 gggtcatttc gggtggagcg gtatattcag ggctgcagga atagtattcc ttgcctatct
     4381 gggttttgat gcagtttcaa cagttgcaca ggaatcaaag aatccgcagc gcgatatgcc
     4441 gataggcatt ctgggttcac ttgcaatagt gacagtactc tatatttccg tatcccttgt
     4501 tctcaccgga atggtcaatt acaagtttct gaatgtcccc gatccgattg ccgtgggcat
     4561 caatgctgcc ggaagcaaac tgttctggtt gagacctata attaaaatcg gcgccatagc
     4621 cggaatgagt tctgtaatcc ttgtattgct gacaggaatg ccgaggatat tatatataat
     4681 gtctgttgac ggcctgctgc cgccggtgat aggaaggatt cacaggaagt tcagaacgcc
     4741 gcatattacc acgataattg ttggttcaat agtagctctc atatcgggcc tctttcccat
     4801 tgatctgata ggtgaactgg tctcaatggg cacactgctt gcattctcga tggtctgtat
     4861 aagcattatc attctcagaa tcaaacggcc tgacatgcat cgccccttta aaacaccact
     4921 ggtatatttc gttgcatccg cgggtgcggc aggatgtctg taccttatga catctcttcc
     4981 gcattcaacc tgggtacgcc tgggcggctg gacagtcata ggcatgttaa tatatttcct
     5041 ctacggcagg aagcacagca agttgaatga aattcccgga aagtccggcg gctcatgatg
     5101 cccggaaagc aggaaatcct gatgaattca ggattttaaa tgtcccggac cccgggggca
     5161 gccgtttccg gaaccgttgc cggcccatac cagttcctga atctccccgg ccagcagaga
     5221 gtgcctcccg gcagccatca tagcaatacg ctttaccatc cggccatagg agtaattttt
     5281 catttctgca gccagggcaa aactggtatc agcactgata tcgttgttgg gattaatatc
     5341 gagaaggaaa aagtcgttgt cacgcagccg gaaatcgaag cgtgcatagt cccggcaccc
     5401 gaattccctc caggttttca gaacggtttt ttccagcttt tcatactgtc tttcagtaag
     5461 gggggcaggt atcagcgtct cgatcttttc gtaatgcagc gatccgggcc tgaacttgga
     5521 atcataggtg cacagccttt cccttgcttc gctaaaggct gagaaatcca tttcagcggg
     5581 tggcagcatt tccggtattt cattgttcca taccgataca tgaaattcac ggccgtcaat
     5641 gaaatcctca accattgcag gctgcttcag caggttgttc acataaagta tctgttcccg
     5701 aagggaagct gtatcgtaaa ctactgattt ttcggtgatg gtaaggctgc aatgttcgcg
     5761 ggaaggcttg acgattgcag gaaaaagatt ccatccgcca gcctcctcag gagcgagaac
     5821 agccccgtaa ggaactttta tcccgaggga ggcgaggcac tcttttactc tcagtttatc
     5881 ataacttagt tctataaccc cggctgtgtt tcctgtacag gtgaatcctc ttccttcaat
     5941 tatctctaca acttttcttt cgctgcactc aatcccggga agcgtttcac agaggttgaa
     6001 aacaattgta tcggcaggat cgtaatgcct gagtattttt tccaggtgat aatcattcag
     6061 ttcttcgata cagacttcaa agccttcttc cttcagtgat tcagaaacca tgctgtttga
     6121 aaatctgacg tccctgattt caaagctatc ccattcggga tcaagatcat atagcactaa
     6181 aaccctgata aaaaattttt gccccgaaga tagaatgaat atctgaaaaa atatgacagt
     6241 cagactgttt tttaactttt tgaataagat tcctgttgat ttctctcatt tttaaataaa
     6301 ttgcagctga aaatatttac gaatcatctt tttttatgag agttaaagga acgcctgcaa
     6361 ggggtttgtt tggaaccaca acaggctttt ttttcggatt tgcggcagtg gcactctatg
     6421 gcccgacagc cgtggaattc agggaggcaa tggatctctc gccggctctg gtgggcctgc
     6481 ttattgccat tccttcactc tccggttcac tgctcaggat tcctttcgga gcctgggttg
     6541 acataagcgg agggagaaaa ccttttctga ttctgatgat aatgagtgca gctggcctgg
     6601 gcggggtaac cctgcttctt tccacttcct atcccgacgg aatgaaggga ctctactggc
     6661 tggttctcct gctgggattt ctcagcggat gcgggattgc aacattttct gtcggaatag
     6721 cccagacttc atactggttt ccgcacagcc ggcagggtat agcactggga acatatgccg
     6781 gactgggaaa tctggctccg ggaatctttt ctatcgtcct gcccctgtat cttacacgtt
     6841 tcggattcat ttcagcttat ttcgcatggt ttctgttcct gataaccgga attatcctgt
     6901 atgcggcaat tgcacagaat tcctattatt tccagttcaa aaaagccggt ttcacggtga
     6961 atgaagcaaa gaccagggca caggagcttg gtcaggagct gtttccatca ggcgggatga
     7021 aggaaagcct ggtcagttcg gctaaaataa cgaatacctg ggcactggtt gtgctttatt
     7081 ttaccacatt cggcggcttt atagcactga cagcctggtt tcccacatac tggaacgagt
     7141 accagcattt tgatccggtt acggcgggcc tgctgactgc tgtcttttca ctgctggctt
     7201 cccttatccg ggtcttcgga ggcaagattg ccgacaaaag cgggggagaa aagacgattc
     7261 ttgtttccat gtcggtaata ctgctgtcag cacttttttt gtccttcctc agcgatgttt
     7321 ttacgagtgt ggtaatggtg atcctgctgg ctgccggaat gggcatatcc aatgccgctg
     7381 ttttcaagct ggtcccggcc tatgttccga aggcagtggg aggagcttcc ggatggatcg
     7441 gaggcctggg ggctttcggc ggatttgcat taccgccggt gatgggagca atagccggga
     7501 aatacggaac cgcaggatat gctagaggtt tcctcgtatt tgtgatgttg tcggtaatta
     7561 acctgctgat aatttatttc ctgataagaa aacacaggac agaaacagta caccggcccg
     7621 gctgatacgg cagaccggct tgcaaaaaga agtcccggag cggtcagatt tcattgctgt
     7681 tgatgatgcg aacccctttc ttctgaagtt cttttttctg gacctcaaaa acttccctgt
     7741 tcagcggagc tgatgcgtcc tcaatcagtg ttgccctgaa gccttcctca atagcatcat
     7801 taatggagtg atatacacat atatccccgg caattccgca gaaatacaga tcagtggctc
     7861 ccttttccct gaggtagccc gacaggcccg tgcttatcag atgcatattg tcatagaaac
     7921 cgctgtagct gtcaacagcc ggattcattc ccttacggaa gatggcagcg atcctgtctg
     7981 tttcaagatc agggtgaaac attgctccgc tgctgccctg gacacaatga tcaggccaca
     8041 gtgtctgttc atttccttcg aggtcgattg tgtcaaaggc cttcttgccg gaatgcccgg
     8101 aagcgaagct tatatgatcc tccggatgcc agtcctgagt ggccagcaca agatcaaaac
     8161 gatgctggat actgttaata aggggaacca ccctgtcggc caaaggtacc tcaagtgaac
     8221 ctccgggtat aaaatcattc tgcacatcaa taatcagaag tgttttcatc tcagtatttt
     8281 ttatgatctt ctatcagtct gtttctttct ttcatgagct tttcgctgag tcctaccttg
     8341 taggcatgag gattgtcaaa ccgtttgtac tcatccggca ggagggccag cctttccgag
     8401 ctgtattgtg ctgtttcctt caggctgaca gattctgatg ttcgctttcc tccttccatt
     8461 accttaagta aaagagcttc cttctttttg ttttttaatg gaaatgactt gtgcggatag
     8521 aggggatgat acattatgtc agtgtccttt tcgctgttga ggactaccac atctgcaccg
     8581 gtacacctgc cgtcgctgtc aaatgctctc agcacctgct tccggtgggg cagtgtaatc
     8641 ttggtaacgt tttcggaaat tttgatccgg ggcttgccgt ccgaatatgc cattttatat
     8701 accccgtcaa gtgccgcatc aggaaaaccg gttaccagac ttgtgccgac accaaaaaca
     8761 tcaatcggcg ccttctgggc tttgagactt ttaatgatat attcgtcaag ctggttcgac
     8821 acagctattt ttacataatc aagtccggcc tcatccagca ttttccggct ctttttggcg
     8881 agataggcaa gatcgccgct gtcgagcctg atacccagca gcctgtgacc tttctcctcc
     8941 agccttctgg ctgttttgat tgcattgggg ataccgctgt tgagagtgtc ataagtgtca
     9001 accagcaata cactgttgtc ggggtgcacg tcggagaaat gattgaatgc cgccagttca
     9061 tcatcaatgc tctgaatata tgagtgcgcc attgtgcctg aaacgggaat gccgtaatca
     9121 cggccggcaa ggacattgct tgttgcatcg aagccaccta tgtaagctgc ccggctggca
     9181 taatatccgc cggggccctg tgcccttctc agtccgaaat cgatcagtgt gtcatttccc
     9241 gccgccttcc gcatacggct ggcttttgtt gcgacaaggg tctggaaatt cagtacattc
     9301 agaagtatgg tctctaccag ttgggcctca ataatatctg cttcaacctg caggaccggc
     9361 ctgacaggaa ataccagatc cccttcccgg gaagagtaaa tatttcccct gaatctgaat
     9421 tgttccagat atttaagaaa ttccggatgg aatccgtttg acctgaggaa ttcaagatca
     9481 tttctgccga atttcagttc ctgaagaatg tccagcaggt tttccaggcc ggcgaacaca
     9541 gcatacccgc tgttgaaagg cagtttacgg aagaaatagt cgaaaactga gatattttcc
     9601 ttctgcccgt tcagaaaata ggcctgagcc atggtaagct ggtaatgatc ggtataagtt
     9661 gccgtgtagt tgaacatgag gcgttaattt gttattattc ccgcaagatt ctgtttccgg
     9721 agttctgaaa ttcttttcag atcagggccg gatacgattg ttaatcagtc tttttaccgg
     9781 cctgaattat ttctgactga aagcctttta caaagttatc acaagaacag gaacaattac
     9841 atgctattaa tactattact ctgaaaaact ttgtttttta gcaaattttg tttgtagcca
     9901 tgagctactt tatgaacaaa aaagaaaatc attttccgga aattacagat atccggcaca
     9961 ataaataaca atttaaaccc ggagttgttc ccgggaatca gacctgaaaa tgcaggattt
    10021 cctgacttat tcaccagtct gcttccaggc tttgcaggta ttctttatcc cttcctcgta
    10081 gggtgtggcc ttcagattaa aacgaccggt aaatttagac gaatcaaaaa aatagtcacg
    10141 gtcatattga tacatcatct cgggcatttc cttcatcacg gggatgaaaa gacccagagg
    10201 gtaaagcatc catttcggga ggaaggaaag tttcggatcc ggaatgccga attcggaagc
    10261 gatcattgat atgaactgct tcccggtgac aggcctgctg tcggttggga ggtgccagac
    10321 ctgaccatag gcatcgggtg tattgcccag gagagccgta gcctttgcag cgtcgggggt
    10381 ataggtaaag gaatgcttgt atccgggccc gataaaccat agcgcttttt tgccggcagc
    10441 aaggtttttc agtatcatct gggtgacaaa gctgttctgg ttatccgggc cgtaaaaatc
    10501 ggctgaccgt gcaataagag ccttcagttg ttttgttttt accgcctcaa ggagcatccc
    10561 ggctatccat tttcttacga cacccttccg gcttgaaggg ttgacaggtg tatcctcggt
    10621 cagttccgcc aagtattcct tgtcatacat atagacattg tcaaagaata ccagttttgc
    10681 attatgtttc agacaggcat caatagtgtc tctcatcagg gcgggccagg aaagctccca
    10741 cacctttgta ctgtattcga agcctatgac aaggtatacc acctctgagc ccgacacagc
    10801 cttttcgacg gctcccggtt tagtcagatc aagggcaaga agctcatcag tttcgttgat
    10861 ccttaccgga ttccggctta caagccggat cctgtcagtg tagcgggtca gttctcctgc
    10921 caggactttc ccgatagtac cgtttgcgcc aagaattgtc tgcatggttt ttatggttta
    10981 gcaactgatg ataaaacaaa tattgaaagg aaaagttaga tcacggtgat gaaataatga
    11041 ttcctgcttt ttaccctgtt gaggtatgcg ttacggcaaa aaatactcat agttgcagga
    11101 tttcaaggca ctatcaattc aagttagcat aaatgatctt ttcccgcata aattttttgt
    11161 taacacaaaa cctgcaatta ccgcttatac aatgttatga attggttttt aataaggacc
    11221 aaagtaaata tagggtgttt cataagcgtt ttttagtttc ttgcttttgt caaataaata
    11281 atatgtagca tacgataaca gctccttaat ttttgtattg ccctgaatat taattttata
    11341 atttgcaacc ggatatttga aatcgtattc agcatttaat gcaatcttgc cttcggcatc
    11401 cacaagtatt aattctttca atgttctttt tttatagtca aatactaaac ccggttggaa
    11461 agtaagtttc ttttttgctt gaacaatttg gattgagtcg ctgccacaga agaataaaaa
    11521 ctgattactc tttatttttc taagtccata tgtatttttt tcagaattat taatgtctat
    11581 ttgctttttg gctatttgaa cagtaacaat ttcattttct gaaattaaat taaaagcgga
    11641 ttgatttttg agatcaaaac tacctgagta aattgattgt ccaatcagta tgccgcttat
    11701 atacaagaag agcatgcctg ttaataatat ttttttcata tctgtttctt ttaatatttc
    11761 tatttgtttg acttttaggt tgtgtcccgt tttcttaagt gattgccggg ctcaactttt
    11821 tttttcaatg ctttaaaagt tgcgaactgt taaacttaaa taccggtggt tctgttgtct
    11881 atttaattcc atttgtccac gaactgacag tataaagaga attaatacca ggcaaacaga
    11941 tattcttttc ataaattaac cgatttagtc aaagatttca gatttttcaa ttcctaaaat
    12001 tgcgttctta cgggtaatca agtaaatgac taagtgaata atgataccaa tcccccagaa
    12061 taaagctgtg acataaacac ttctaatgct ttcatagtgt gaatgaaaat caaataaaaa
    12121 ccataagatt gatacaatat tgaaaagtaa ataagttgca aaatgaaaac ggaatccaat
    12181 gtcataaaga ggtagagttt cataatcctt tttaccgaat atccaaccta tgaaaaaatt
    12241 gtagaaaaaa tagattaccg caataatcca taccagatca taagtgcgat tttctataaa
    12301 gtaactcaaa ctatagcgaa acataatagt acctatgaaa aagaaaatca taaaaaatat
    12361 aagatttttt gttaaagctt tcatttttag tcctccttgg taattaagtt tttaatatgt
    12421 ttattaaaaa acaatgagat atttctctca ataaaatttt ctaaaagtct atgacctatt
    12481 tctgtcaaga aatagtattt acgctcagga cctttgttgc cttcgcctgt ttcaaaatca
    12541 acaactccta agtgttgaaa ctttctaagt gttcgataaa ggctttgact ttcacaagtc
    12601 attgtctgat tagactgttc ttcaataaaa cattttattt catcaacata ttttttgcca
    12661 tctttcaatg aaagaaaaat ccatagagtc aattgcccct ttttataagt ttcctcccaa
    12721 gttgataata attcagataa gtaattagtc ttattcatga tttattcatt atgttataaa
    12781 caatatatat aatacataat gttataaaca acatgtataa tacaaagata ataatacaat
    12841 tttgaaaaac aaataaattg caaaaaaatg ataaaaataa atcacttgcc actaacaagc
    12901 ggtataaaac aatatggttt aagtggttat gcaatatttc tacccggcgt caaagttcga
    12961 tatcggggga tactgacgta atccgcaatc cccaacgttt cataccgcca aaccgttaca
    13021 taaccggaaa taactcgcag agttataaca agagtttgga ctttactgga agagtttttt
    13081 ggaatcagaa ccgggtaaaa tttatttggc cattccctgg gatcaactta attgaacgcc
    13141 tcagcttcga tcccctcaat gaaggcaccc ggttaattga atcagtgagg tacagccgta
    13201 gtttgttcgg gaagatcact cacgtcccgg ctgatgacat ttatgcaacg aatgccaaca
    13261 ggaagtgggc acccgtcagc atatcaccac aaacttcaag cgtaaaggac gagcaggcaa
    13321 gtacgaattc gaggtacaca tggccaatgg tacactaaag aatccgatca ggatatcaat
    13381 cacccaatcg gattttttga aatattgttt ctgaatcttt ttacggtacg cggagtggca
    13441 gacagaccgt atccggggaa ttgggaaacg ccttagagtt acagaaaggg cgcttaaagc
    13501 gcgcggaatt aaaaaactcc cggatggttt atactttccg ggagtcatgt aataaattag
    13561 aaaaaagtat tccgtcggga tttatgatat gttgtaataa ctctcccatc ctttcctggg
    13621 aggcctgtct gtcagcaatg aattcgcgaa cttgtcattg ataaattcct ttgtttccct
    13681 gttgaacaat agttttttat tccggcggac cgctattatg cccagggaaa atatctgtga
    13741 aagtggcgca aatacctgga agggtgaacg tgttttttct tcacccatac aggctttcag
    13801 gaagttgaca taatgatccg aggggctttc aggaacgggc ggcaatttcg gagccatttc
    13861 ctttgccttt gcctcaggga taataaacag gggagcggaa tgtgtgcctc ccttgaatgt
    13921 aagctctttg ctgtaaatga tttttccggc ctggggtctt ctgggctgac cgcctgcctg
    13981 ctttgccctt tcctgacggc tcactcctcc gggaggtggt atggtactgt caaccgttgc
    14041 cgaaacagaa tcatatcctt cagggaaggg cggaaggttg tccacaccgt cataccaggt
    14101 tatatcgcat gggggcatat ccccgcggct cgggaaacgg aaacggagcg ttgtttcaag
    14161 cgggaagaaa tagtcattga ggtttttaac atgaataggc tctatttcat atggtaaacc
    14221 aaggtctaga aattcatgta ttgtgtcaag gatgtgggct ccccagtcgc caagtgctcc
    14281 aagtccgaaa tcataccagc ctctccagtt gcccggatga tatttttcat tgaaatcgtg
    14341 atatgcagcc gtggtaagcc agacatccca gtcggtttcg gtcattcccg gcggcaatgg
    14401 ctgtgcttca ggatatctgc tgatgtttgg atcatatgga tgccagcgcc ggctgccgtt
    14461 catgaatgcg gtaacggccg ttacatcccg gattatgcct gcatccttcc aggccctgaa
    14521 ctgaaaatag ttctcccccg aatgtccctg gttgcctacc tgcgtcacaa ttttcggatt
    14581 attttcagcc attcgggcga gaatctctgc ctcaaggaat gtccgtgtca ttggcttctc
    14641 aacaaatacg ctcttgccca gctgcattgc tgccatacat accgggaaat gtgcaaagtc
    14701 gggaattgct acctgtacag catcaatatc acctgccatt ttatcgaaca taaacctgaa
    14761 atccctgaac tgctttgctt tgggaaccag ctttatcgtt tcctggcagc cttttgcatc
    14821 aaggtccaca tcacaaacgg caacaatgtt tgccagacct gaattgatga attgccttgc
    14881 cactcctgct ccctgatttg caatccctat cagggcgaga ttaacttttc ccgtccggcc
    14941 tgacaatttt ccggagggta atatttcagg accccggccc tgagcaacgg ctgtcttgcc
    15001 taatacaaat cccgatgcag ccagtcctga agtttttaaa aagtcgagtc tggataattt
    15061 ttctgacata acagaggctt taaaattttt acttgaacag gtcggttaaa atgaattttg
    15121 aaaagatcgc ggtttttata caatatacaa ttataataat ttgtcaggaa aaaataaagg
    15181 aattgctgtc agtaaattga aaaagactgt ggtcactacc ctgccgggga ccgggaccgg
    15241 agatctccgc tgtttatgtt cagccagccg gttgtaaggg aaataaacca gaaccggata
    15301 atgtgagaaa catatcggaa aacacaacgg atatgataac tgtcatcttc tgagacgatg
    15361 gtcatggaca tcggagtcca gtcttaccgt ttcggtcacc agttctttaa tattccattc
    15421 cctcacggct gacacctgtt tttgtatcag gtagaggttt gacattatcc tgccggtcat
    15481 aaaggcaata gctgcagcga ctaccccgat aaagttcaga aaaactgtac agacaagcaa
    15541 agtcattatg atggtgccga gctctattgc cgttgccacg gaaatggggc gtgtggtacc
    15601 gtttataatg agcgaggacc tctggaaatt cagaagaact gtaagcgcag gcacgagtat
    15661 catgattttc aatggaagat atgacatatc ggcaagatcc tgactcagtc ccgatatatt
    15721 tatgaaccac aggtttgcca ggggagtgaa agccagtacc gaaatcagga ccgtcaccac
    15781 caggccaagg aaaatggcat aattcttcag aagcctgtaa ttctgctttt ccttgcctat
    15841 cagtgcaatg ttcacttcct ggtatgacag tcccatgctt ctgaaaataa ataccagcga
    15901 gctcaccacg ggcagaacgg ccagtgattc cacagccata cggcttcttc cgaggaaaaa
    15961 ggtgacaaag ggctggactc cgagcgagag tattgaagta agggcaaggg ggaaatagaa
    16021 tcgtgcaact gaccttaagc ggagattgcg gttttctgta tcgtctatct gaagcagtgt
    16081 cttcagcgtg cgggaaacca tcattcttgt tgcaattgct tcaagaaaaa ctgccagtga
    16141 cattgccccg gcaccgacat aggctccttt cacgcctgct gaataaagga ttactcccat
    16201 tatcattatc accaccagcc tcacgacagt tccgtaagtc accctcctgg tgagattatt
    16261 cctgatcagt attccctgat agaacctcct gtaaccgatg gatgcagccc agggaagaaa
    16321 aatgaaaagt gccgtgtgcg ccggtcttgc gatatcctca ggcattccca tcagaccggt
    16381 tacgataaag ctgaaaaccg gcggaataag gatgatcagc tgaatgactg tgatgccggc
    16441 attgagcagg tcagtgaatc ttttcagctt cctgtatgaa tgacgtcccc tgacaagagc
    16501 agtggaagca ctcattagca tgattatcgg cgactcgatt atcatggcaa aagatccggc
    16561 tattccaaaa gcggcaagat tgaactttgc ctcattcagg cgggcaatga aggcaatgag
    16621 gaaaggctgc tcaaaagcca tcatcagcca ggtaagcgca agcggccccc agaaaagaaa
    16681 gattttcctg tatgtcagct tttcagttat gtccatttcc cgggaaaagc gcaaagatag
    16741 gaaaatatga aagcagatga atagtacggc ttcccggcta tccggaagca tttacagatg
    16801 aatgccgttt ccgtcaagaa tggcatgatc ccggccgctg cagctgaaac aggtaaagga
    16861 atcaccctct tccgttgttc tcgatctcat tacactttca ttgcagagcc ggcataccac
    16921 gctctcatca attccggcat aatccggtat tgcggtatga acccgcttaa cagtgaataa
    16981 acttccgaaa ggaagatcca gggtgccgaa agccctttcc agggcagact tcttatatgc
    17041 ggcaaccatg tcaggatcac gtttctgctc agcaatgact gaccggtagt atttccggaa
    17101 atccgggaat gcctcttcta tcacatccct tgattcatgg cgcgaacaga tccttattcc
    17161 tttaccgtcc cttccggcaa gcgtaaatgc tgtttttccc agatccttgt agatcaggga
    17221 attattgccg aatgagcagc ctgtgacaaa ctgcactccg tcagaaaaac aattgtttgt
    17281 ttcggttata gccagaaggt tctccatgcc gtcggaatca gcctgcaact ccctcatggc
    17341 atggacggct gccatgactc ccatggcaag tcccggacag aaatgaccgt gcatctgtgc
    17401 agcctttata agcaatatat ctgtttcgcc ctttgctatg ttgtttctga tttcaattct
    17461 cggatcagat ttgagaatcg aattatgaac ggggtttgat tcggtgactg aagcaaacaa
    17521 tgaggtgtca cagtttttta tatcaattac aggtgtgtta ttgatggcat caaggccttc
    17581 cacaatgagt tcgttatttt tccgctcaat cagttttact gttgtaatcc ctatcagatt
    17641 gggcctcctg ggactgcggg aggcaaacac acccctcacg gcacctgaat gggtttttcc
    17701 ggacagggtt ccctgttccg atttatgaaa ccagaaaaca atatccagat attcacactc
    17761 atcaatgttc agcagggcct cactgtattc atcatgcact ataacggagc tggggtgttc
    17821 ctttatcgtg ttgtaatcgg cgggattgtc gaatccgttg cgtaccaggc ctactggtct
    17881 gacagtgtat gttttcatat ctgaacagtg ttttatttta taatccttcc ggcaggataa
    17941 atgtatcatt gtcatttctg ataatcttta ccctgatatc ataaagattt tcaatattct
    18001 gttttgttat tatttccctg ccgccgctgg caaatacctt tccgtccctg agcatcatta
    18061 ttcccgaggc atagcgtaca gccatgttta tgtcatgcag ggcgatgaca atggtgatgc
    18121 ctctggcgga aagctttttc agcaggttca gcacttcaat ctggtgtctg atatcaagat
    18181 ttgcaattgg ttcgtcaaga agcaggatat ccggttcctg ggccagagcc ctggctatgt
    18241 acactatctg ttgctgtccg ccgctcagtt tattgaaatc cctcattgcc agatgatcaa
    18301 gattgagagt attcagtatt tccgatgtta ccctgaggtc gtgttcagcc ggtttccagt
    18361 tgatataggg tttccttcca agaaggacaa tgtcaaatac cttaagtact gccttcctgt
    18421 tttcactctg gggaacatag gcaatctccc tggccagttc attgggactg aacatggata
    18481 ttgccctgtc atctatcagt acagtgcctt tgctgacttt cagtattccg ttcatacact
    18541 tgataagggt acttttacct gatccgttcg gaccgaccag tgccacaaaa tcacctttct
    18601 caattctgtt gttcacattt tcgagtgcag ggaagccatt ataactgaat tcaatatcct
    18661 gaatggtgat tttcatcgtt taaccggctt taatgatatg atgaaccccg ggaaattttg
    18721 cgttttgaac ggttgcggct ttcatctctg tatttatatt gactggcaaa attaagacaa
    18781 aactgttctg taatattttc aggtccagta atcctttccc attcctttaa taagcagaaa
    18841 cagaaacagg ggtgcacctg cgaaagatgt taaaattccc acaggaagga tgaccgggga
    18901 tatgattgtt cttgcaagag tgtcggaaaa cagcaaaaat gttcctcccc ctattgccga
    18961 tgcaggtatc aggaactgtt cgtcgcctcc gatgatcctg cgtacaatat gaggaactac
    19021 cagtcccaca aaggcaatga ttccgtaaaa tgaaacaaca actgctgtca ccagtgaagc
    19081 cacaaccagt cctgtcaggc ggactcttga aacattgact cccagacttc ttgcatattc
    19141 atctcccgac tgaagtgctt tatagttcca gctctggctt ataaaaaaaa ccatgacaag
    19201 actcactacg actgcaatta tcagacagtt gttccatgat gctctgccaa tgtcgccgaa
    19261 agtccagaat acaattgatg caagctggat attatcggaa aaatactgca ttgccgatat
    19321 gcctgcacca aacagtgaag aaataattat ccctgacaat atgattattt caggtgttgc
    19381 acccttatac tttgagagca cgatgatgac ggcaacactg cagaggctcc acaggaatgc
    19441 ggaaaccgtc accacatagg gattattgat tatggccata tcgttggatc cggcgtagct
    19501 gctgccggct ccgaggacaa ttattgcaaa ggcagcgccg aatgcggatg ctccggaaat
    19561 acccagagtg aagggggaag ccagtggatt cctgagaatg ctctgaatta cggctcctga
    19621 cacggaaaga gccatacctg ccagaaaggc tccggcaatt cgcggcaggc gtatgttgta
    19681 gatgatctga cgtgaaactg cctgtcccgt tcccgttata aggctgtata tatcccttaa
    19741 acctatgtcg gccgatccgg aaaaaagcga gtatatgata ataatgaaca gcagcagcag
    19801 aagaaagagt atgaaaagta ttctcttctt cctgaaattg taatattggc ttaaaacctg
    19861 ttctgacatc aaagttcgtt tttactgata ttcctgaaag ctgatgaaga gggttttatt
    19921 tcatccgata tatccctgcc caggaatatg cggtatactt ccttcgtttt ttcatcggca
    19981 tcaatgtcac tgaatttttc gggatagagg atttttccgg caaaccatgc atttgccagt
    20041 accatttcat agttggttgc ataattgtta tagggcagaa gggtgtacat cctgttttcc
    20101 tctacggcct taagcctgtt gaataatgct gttccccttc ggagatcctg ctttaccagg
    20161 ttaagtcctg attcatcaat gaaaagaata tccggattcc agagcatcag ttgttccgta
    20221 tcaatatgag tgcccttcac gtgggatatg agtcttttgt caatctctga tgccggactg
    20281 cgggcattca ggaacatgaa aggagggaag aagggctggg ttgaattgat gccatatgaa
    20341 cccgaatagc cgactccgcc tatgtaaacg agtggttttt ctgattccgc tgttccggat
    20401 gaccgtctgt tcagctcagc aattgaattt tcaatgtagt ttatcagtgt atcggccctg
    20461 ccgcttctgt ccatgatctt tcctatgagt ttaagggaag caaaaagggt atcccttgcc
    20521 gtgccgaatt cagtacattc gagggcaatt accggaatgc ctgttttctg ctgcagggca
    20581 tcagcatcac ctgctgtggt ataggtaagg aaaatcaggt caggttttga tttcagtatc
    20641 agttcggcat caccgcccat tgacggaccc agtgaaggca gctgggccag ttcgggatgt
    20701 gctttcatgt agggcgtgct gccccgtttc tcattctgtt ccactccggc aatcctgtcg
    20761 gcaaggtcca tatataccag cagccgtaaa gctccggcac gcagaccggc gacacggttt
    20821 atgcgtgaag gaatgaaaac ctccctgcca agtatatccc ttaccgccac agatccgtta
    20881 ttccctgctg gctgtccccg gctgtccgaa gacagcctgc tgcctgcagc gatgtgcct
//
LOCUS       DAOSPX010000017        20544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2784_length_20544_cov_5.236468, whole genome shotgun sequence.
ACCESSION   DAOSPX010000017 DAOSPX010000000
VERSION     DAOSPX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20544
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2784_length_20544_cov_5.236468"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLZ75_01790"
     CDS             <1..382
                     /locus_tag="PLZ75_01790"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOS71161.1"
                     /translation="ISNRGKVQGETLRDLSFSILQATEARITRNRYISCPTCGRTKFN
                     LQDAVRKVKEATSHFSGLKIAVMGCIVNGPGEMQGADYGYVGAGIGKVHVYRGMVPVY
                     KNVPEEEAIARLLELINADLNPDY"
     gene            complement(453..869)
                     /locus_tag="PLZ75_01795"
     CDS             complement(453..869)
                     /locus_tag="PLZ75_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOS71162.1"
                     /translation="MMVHRLLTAYLNGDNPGERKKYEKMCEHSSKMERLAAEAERASI
                     KYKQVEFMSDKTGRKYEGIISGVTEWGIYVEIIENQCEGMINIRELDDDFYEYDEDNY
                     CLRGRSTGRVYTLGDKVMIEVVKADLQKKQLDYRLA"
     gene            complement(884..2581)
                     /locus_tag="PLZ75_01800"
     CDS             complement(884..2581)
                     /locus_tag="PLZ75_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOS71163.1"
                     /translation="MSRKGKGKRKSGISAEWIREKILTVLSGNNRQGLNYKQVAKRLN
                     ITDPAEKQLVAEMLRSLKKQEVVEEVYPGKYRTRAACSYVNGTVDMTRMGYAFITGDD
                     IKEDIFVSGRNLNTALHGDRVKVCLFARKKGARPEGEVVEILERWRSSFVGTVEVMPG
                     FAFLIPDNKNMHSDLFIPLSKLKGARQGQKAVAKVIEWDSRSRNPVAEIINVLGEPGM
                     HETEMHAILAEFELPYHFSEEVVKEAGKIPAEITEKEIKSRRDFRSVPTFTIDPADAR
                     DFDDALSLQKLDNGNWEAAIHIADVTFYVSPDSLLEAEAQQRATSVYLVDRVVPMLPE
                     RLSNDICSLGPGKDKLTYSAVFEMNDRAEVLNEWFGRTIINSDRQFSYAEAQNVIDTG
                     SGAMKDQLLVLHDLAQKLRKKRFASGSFAFEKVEVEFDLDEAGRPLAIKFRDMGTANQ
                     LIEEFMLLANRRVAEFVSRKLRGRTFVYRVHDKPDPEKITNFANFISRFGYRLEGNDG
                     VPLPKAMNRLLTSVAGRKEQNIIETLALRAMAKAVYSTDNIGHYGLAFKHYTHSPRP"
     gene            2812..3099
                     /locus_tag="PLZ75_01805"
     CDS             2812..3099
                     /locus_tag="PLZ75_01805"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOS71164.1"
                     /translation="MINLKSVPRRSESIVTRKTGNEYVLVPVTDNIADMNSVYTLNDS
                     GAFIWELIDGKRDIEEMIEALVSRYDTDRETASEDLLSFIGKMSEYLVIDR"
     gene            3170..4177
                     /locus_tag="PLZ75_01810"
     CDS             3170..4177
                     /locus_tag="PLZ75_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71165.1"
                     /translation="MKSYVLNIADYKIRFENPEGNIDLMPSGRYSGFLSATENHDVCI
                     RVFPGDTELPAGTQKVFSAPYVEEINGLRLKKKDRFWSVHKKDDTLYILTEFPFSGDN
                     RTGLLRFSLESRNWDLFLNSSSIRTGSSDQVFGADPMDYLPDRGCRAEPADGAVSPPQ
                     ADPMDYPLDGLILYYLTVIHGDIMIHASGINSGGKGFMFSGVSGKGKSTMAGLWDRHG
                     ARVIHDDRLIIRKVRGSYSMFNTPVYRNDEARESPLDAIFIIEHGTENEALRLHGASA
                     ITSLLSNCIQHNWDEAVIRQSLDAAIRICASIPVFQLRFRPDNSIIAYLLQQVKTNES
                     G"
     gene            4197..4631
                     /locus_tag="PLZ75_01815"
     CDS             4197..4631
                     /locus_tag="PLZ75_01815"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOS71166.1"
                     /translation="MEKSGNDHKIARDLGFSLLSEGRILRIRAGGYSMYPSIKPGTTI
                     LISPKENMKDIVPGDIIAWKRAQGFVVHRLIRTEERGNEILYYTRGDSCRHEDSPVTG
                     EQIAGKVIRTEDRKNRIKKGDQLRKKPAYPLNRIIVRFISGI"
     gene            4681..6468
                     /locus_tag="PLZ75_01820"
     CDS             4681..6468
                     /locus_tag="PLZ75_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS71167.1"
                     /translation="MNNKFKYFRESLKLVWQSAPGWTFANIMLSVLRSILPLLLIWLL
                     KNLVDELTKAVSLNSPEVLPSIIWLIAAVVIIWFVDEATSDLGNYVRKKQSMKLEIYM
                     YGLIHSKSVRLDLLNFEYSAYFDSLSRASREAPWRPNSILNNIVSLFRSLLSLLLMAG
                     VLVVLHWAVALLLVVVNIPGIWLRLKYAGTLYNFQREQTPEARKAAYFNWLLTGDRPS
                     REIRLFGLGNYFTALFKKSFLRTKEEEIRIIRKRTAIELVSDLVKAAAVMITLVFMAR
                     ETTGGKLTLGHMTMFVLAFRQGMMYIRELLGSLAGLYEDSLFIGDIFEFLNLEEKIKA
                     IPPVADIREFSSGISVNNISFTYPGNNSHTIKDVSFSIRKGEVVAVVGPNGAGKTTLV
                     RLLCRLYDPDSGSISIDGKDLKNLDPGQYRKLLSVVFQDFMLYNLGAGENIRLGNISK
                     SDAPEDIIKAARMTGIHDLINDLPDGYSTVIGNLFSDSRELSWGEWQKLAISRALFRD
                     APLLILDEPSSSLDADTEYEIFSRFREIVSGRTSILISHRFINVSLADRIIVLSKGSI
                     AESGTHDELMEKKGIYHSMYTKQTSRFRR"
     gene            6465..7634
                     /locus_tag="PLZ75_01825"
     CDS             6465..7634
                     /locus_tag="PLZ75_01825"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS71168.1"
                     /translation="MTESLNLRTEEQLLCGLCRLSFSSAQTEKLKSCIGNTNDWDYFL
                     SLARNHGVSALVYHNSNRLGLTDLIPPAVTGSLRNSYMINLSRNSGFLVKMPPVLKLL
                     NSRNIKTVLLKGLALELTVYGNSGVRQMTDVDILVSPENALAARQVLIENGFVSKPLK
                     SVLYKPLLTCSGKHLPSLSAEGLSVEIHTGLFGKKNSCLTRKLYDESPEKDLGGEKAY
                     IPSPLMFFAYLVRHLWLHEMKNESQLRLYADLVVLIEKYHGEILGPSLTGLCEKAGMN
                     EIMATRLRPLRDVLEISFPDDINNFIDEWAAPDSLDKFLFFLKSPGNNPPVSRARLYR
                     YHLGEIPGFHRKLLFIAGDLFPSFEFMKNRYACRSRLETLMYYPLRLGKLWYLIG"
     gene            7957..8187
                     /locus_tag="PLZ75_01830"
     CDS             7957..8187
                     /locus_tag="PLZ75_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71169.1"
                     /translation="MTKKKRELTKDSILNLIRLRAAGTPADLAGRFGVSERSIKRMIS
                     ELKLEGYGIVFWHGGNTYILENDMDQDIKRAV"
     gene            complement(8664..8846)
                     /locus_tag="PLZ75_01835"
     CDS             complement(8664..8846)
                     /locus_tag="PLZ75_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71170.1"
                     /translation="MENKKSLVPVIVITVSAIIVLFALLIPSAKPLPWLILAASLFYL
                     TTGWYMFSICEFTKQV"
     gene            complement(9051..10022)
                     /locus_tag="PLZ75_01840"
     CDS             complement(9051..10022)
                     /locus_tag="PLZ75_01840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS71171.1"
                     /translation="MKYRTFGRTGWKISEIGYGTWGMGSWTGSDDEESMRSLQLAADL
                     GCNFFDAAWAYGRGKSEDLIGQLVRANKDKKLYTATKMPPKNFKWPARREYTLEDCFP
                     PDHIQEFVERSLKNLGLDSYDLMQFHTWEDGWLEDERGLKKMMELKEQGLFHAIGISL
                     NRWEPWNGIRAVRSGLIDSVQVIYNIFDQNPEDELLPACREMNVAVIARVPFDEGTLT
                     GTLTKESTWPKGDWRNSYFIKENLDSSVEHADALKPLVPEGMTMPEMALRFILGEPAI
                     STIIPGMRKESHVRSNIATSDAGPLDDALMKELRKHRWDREPSEWSQ"
     gene            complement(10136..10906)
                     /locus_tag="PLZ75_01845"
     CDS             complement(10136..10906)
                     /locus_tag="PLZ75_01845"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOS71172.1"
                     /translation="MKNKATDTDIRSVLNFRDAGGLNLTGGGRMRKGLIYRSASPDRI
                     SRRDLKKLQALGIKTVADLRDPAEFSRRKAELPGIRIINYPLSFESVTRERLVPLMKR
                     RYDAGAINKVINGLYVDIVDAVRPVLGKTAELMLEPGSTPVLIHCQAGKDRTGILSAL
                     FQMIAGADRDEIIRNYMASNDSLVPHYEKRLRVRKFITLGFFPADSVLHAVTQKKEDI
                     VTVLDRIEGHYGGIIGYLSGSGFDVSKTDKLRDMLAEK"
     gene            11184..11939
                     /locus_tag="PLZ75_01850"
     CDS             11184..11939
                     /locus_tag="PLZ75_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71173.1"
                     /translation="MKKIGLLFLTAILIITVSCSKSDGDTGILEFKGLKAFQETSHKS
                     SEKSARAAFADGPQTHKMHTADMKFDIAEIWVSQDMVEDNIKDNLTWHLIGRNKGIKS
                     CEDYSFSASKLPVGEYKSIKMVFRNRIIRIAVYADNFSKSVEMNSSLDEESEGDDSFI
                     TEYFCPNGSFILSGGMIKLMSTGEKISGFTIRSGEKTILSWKLGGPDSKITDCSFDWV
                     DTNGNGQWEWDSDEITNDHCSVEMPMFSFIVEE"
     gene            complement(12068..13291)
                     /locus_tag="PLZ75_01855"
     CDS             complement(12068..13291)
                     /locus_tag="PLZ75_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71174.1"
                     /translation="MDRLADRGIIQWEDIFVFLYSGASILALISFFFPEYKSCKEPFS
                     KIYPINNFTRALTGLISDCMSRFIIILAAVILISALFFKVPFPGRMRYAGCLLTGVLN
                     AVILSRNLRFLVEKDFYKSIHRIPGWLLMVIIYTVFLFFIFRYIDSVIFNVLTVVAGF
                     GLQLVLNKWTYDRRISDRGSHLPFVIQLYIRNKIFRVSVLIMFAVKIIIILFFFLLLE
                     IDPDANTAFKIPVVFYSSPVLVFTYLFNNFFGFFPAFITNFKISGTEYDSLTGLLLKT
                     VLPLILLDFLLSFAFIFYGRYDLQAWLLFYFSVLTVLIPVSLHLSTFWPNPVKAAFSS
                     SNNRPMLNSFSMIPVLAASVCYLYFQSLLPVIFLLYFTISVFTARSFIARYKKNFPHP
                     CIHYLFQAAEILPGI"
     gene            complement(13436..14122)
                     /locus_tag="PLZ75_01860"
     CDS             complement(13436..14122)
                     /locus_tag="PLZ75_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS71175.1"
                     /translation="MIVLQKVYKSYKDKKVLINLDLSVERGQIFGLTGKNGSGKTSLL
                     KIICGLEKPDSGNVRIINNTTGKPVIGVSLYETSQLINELDGYTFLNFIGRIYKIPLF
                     ETEKRILELSDIFEFSDVLYKPIGSYSTGTRKKMSICTAIFHKPQLLLLDEPFENLDA
                     TACRKLITLLKAYISPEKTIIITSGQTGHLQQLTNNIAVLSNGRITDLHADPGFQKIG
                     GGNKILPETE"
     gene            complement(14149..14859)
                     /locus_tag="PLZ75_01865"
     CDS             complement(14149..14859)
                     /locus_tag="PLZ75_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS71176.1"
                     /translation="MIKAENLLKKFGNTIAVDIPELSIENGDIWGLVGNNGAGKTTFL
                     RLMVDLLKPDRGQILSKSIPVYQSEHWKNYTGTFIDNNFLIEFLTPKEYFEFIAKIYD
                     LEKNEYYERLQGYNHFLNSEISETSKYIRQLSSGEKHKVGVIGALLPDPEVLILDEPF
                     NFLDPSSQIVLNKILNSGFRDKNKTLILSSHNLNHISDICTRVALMEKGKIIRDLQNN
                     RESMSEVEDYFRYQTNNQ"
     gene            complement(14856..16322)
                     /locus_tag="PLZ75_01870"
     CDS             complement(14856..16322)
                     /locus_tag="PLZ75_01870"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HOS71177.1"
                     /translation="MLTEVIKHSIKSFLRNTTFDLRLILRLLMILSILYLFSFLVYLG
                     YGFSGFLYLYSPSSDPVTFLNNRLLHIGAVLFILQVFSIKNPLKALLAYLHLPIKRNK
                     LVLYILFLSQFNYFFIGLLLFFVPYSIRTILPVYSIQQFFFYFPGVLIIFFSVGYFSL
                     LIRNLIGISYSYIIIPILLFGGLYLMDLFLDISFKDMSRFIFKQLINKNLYFLILLIL
                     ILAGLLICNFILLKKGFYGIYQNQKSLLNYSFRPASHYFRENDLFTYVLLEIRLIARN
                     RRTRGFLLIILFFSIMFYRILPYDGNLYVIFILFILISGMFGYTFIQFLFSWESGYFD
                     FILSTKFDIIKYLKAKYAVYSIFSVILFTVYFPVIKPVQTGLHMFFSALLYNSSLGYF
                     VLIFLATYNKFKVDPNRSILFNYQGLNSFQVIGVLLVLLIPILTLFLLLLRLNLTHSL
                     LIINLLCIIALIIQGKLWKIIQNKLMRRKYINLEGYRK"
     gene            complement(16353..16529)
                     /locus_tag="PLZ75_01875"
     CDS             complement(16353..16529)
                     /locus_tag="PLZ75_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71178.1"
                     /translation="MIDLTFIHEGKLLLAWVWGTGIIGNSVCRSGKYIFLLHEIAETI
                     FIMEGDTWYFSANR"
     gene            complement(18158..18895)
                     /locus_tag="PLZ75_01880"
     CDS             complement(18158..18895)
                     /locus_tag="PLZ75_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS71179.1"
                     /translation="MISLKSVNKSFNGNSVLEDINLSLERAQVYCLLGRNGAGKTTIL
                     NLILDLLTPDSGLIEVLNKPGNKLDNQSKARIGVLREDLAVINELTGFDYLTLTGHFY
                     NMSGIAADHKISELADYFFDEKEVELKKRIGTYSFGMKKKVAIIAALINEPDLLILDE
                     PFAGLDPVSAKRLVTYLKNYISPERLILLSSHNLNYIKQISTHIGIVHKKRLFLDSKI
                     NDFPVREGESFEDAFCRLIETEIETEI"
     gene            complement(19542..20291)
                     /locus_tag="PLZ75_01885"
     CDS             complement(19542..20291)
                     /locus_tag="PLZ75_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS71180.1"
                     /translation="MEEIINFNSVIKRYGKITALNGLSFSIKKGESVGLIGNNGCGKT
                     TTINVLCNLVNYDSGNVLIYGNKIIPGFVTYKNKLGILLSPPIFVNEFTPIQYLKFVC
                     KFQKVDQFEIDLRIESLIKSFNLNSFNKKKIGDFSSGDKMKIALAAAIIHNPEVLVFD
                     EPFIHLDFQTIDFISSLIKSLRNKKTLFITSHNIDLMTNLCSRFLIMDHGQIINDIAL
                     HENMSHNEIKSLITDSILKQNIRSINMDWFE"
BASE COUNT         6332 a         4264 c         4095 g         5853 t
ORIGIN      
        1 tataagcaac aggggaaaag tacagggaga aacactcaga gatctttcat tctcaatact
       61 tcaggcaact gaagcaagga taacccgcaa ccgctatatc tcgtgtccca cctgcgggag
      121 aacaaaattc aatctgcagg atgcagtcag gaaggttaag gaagcaacaa gccatttcag
      181 cggattaaag attgcggtta tgggatgtat tgtcaacgga ccgggtgaga tgcagggagc
      241 tgactatggc tatgtagggg caggcatcgg gaaagtgcat gtatacaggg gtatggtccc
      301 ggtctataag aatgttcccg aagaagaagc aattgccagg ctgctggagt taatcaatgc
      361 tgatctgaat ccggattact gaatgccaaa tggaaagaat acctgaagta aagtcttttg
      421 cttccggaag cttcagaaag gaattcctgg aatcatgcca gccggtagtc aagctgtttt
      481 ttctgcagat ccgccttaac cacttcaatc ataaccttgt ccccaagggt ataaaccctt
      541 ccggttgagc gtcccctcag gcagtagtta tcttcatcat attcataaaa gtcatcatca
      601 agttcccgga tattgatcat gccctcacac tggttctcaa ttatctccac atagattccc
      661 cattcggtaa ctcccgaaat tattccttca tattttcttc cggtcttgtc actcatgaat
      721 tccacctgtt tgtacttgat ggatgccctt tcggcctcgg ctgccagtct ttccattttg
      781 gaggaatgct cacacatttt ttcatatttc ttcctttcac cgggattatc accgttcaga
      841 tatgcagtta gcagcctgtg aaccatcatg tcggggtacc ttcttatggg cgaggtgaat
      901 gagtataatg cttgaaagcc aggccataat gtcctatgtt gtcggttgag taaacagctt
      961 tcgccatggc tctcagtgca agcgtttcaa ttatgttctg ttccttcctg cctgcaactg
     1021 aagtcagcag tctgttcatc gccttcggaa gaggaacacc gtcattccct tcaagtctgt
     1081 agccaaaacg gcttatgaaa ttagcgaagt tcgtgatctt ttcaggatca ggcttgtcgt
     1141 gtaccctgta aacaaaggtt cttcccctca gttttctgct tacaaactct gcaacccttc
     1201 ggtttgcaag gagcatgaat tcctctatca gctggttggc agttcccatg tcacggaatt
     1261 ttattgccag gggccggcct gcctcatcca gatcaaactc cacctcaact ttttcaaatg
     1321 caaatgaacc cgaggcaaat cttttcttcc tgagtttctg agccaggtca tggagaacaa
     1381 gaagctgatc cttcattgcc ccggaaccgg tgtcaatgac attctgtgct tcggcatagg
     1441 aaaactgcct gtcggaattg ataatggttc tcccgaacca ttcattcagg acctcagccc
     1501 tgtcattcat ttcaaacaca gctgaatagg tcagcttatc ctttccggga ccaagtgaac
     1561 atatatcgtt tgaaagtctt tcaggcagca taggaacaac cctgtcaacc agatatactg
     1621 aagttgctct ctgctgtgct tcagcctcga gtagtgaatc cggactgaca tagaaggtaa
     1681 catccgcaat atgaatggct gcttcccagt ttccgttatc aagtttctga agggaaaggg
     1741 catcgtcaaa atcacgtgca tccgcaggat ctattgtaaa tgtcggaacg gaccggaaat
     1801 cccttctcga tttaatctcc ttttccgtta tctctgccgg aattttaccg gcctccttca
     1861 ccacctcctc ggagaaatga taaggcagtt caaattccgc tagtattgca tgcatttccg
     1921 tctcatgcat gcccggctcg cccagtacgt ttattatctc ggcaacggga ttccttgacc
     1981 tggaatccca ttcaatgact tttgcaacag ctttttgtcc ctgtctggcc cccttaagtt
     2041 tcgaaagagg tatgaacaag tcggaatgca tgttcttgtt gtcggggatc agaaatgcaa
     2101 atcccggcat cacctcaacg gtcccgacaa aggaactgcg ccatctctcg agtatctcca
     2161 ccacctcccc ttcgggtctt gcgcccttct tcctggcaaa caggcatacc ttcaccctgt
     2221 cgccgtgtag ggccgtgttc aggttccttc ccgacacgaa tatgtcttcc tttatatcat
     2281 ctcccgtgat aaaggcataa cccatacggg tcatatcaac agttccgttt acataggaac
     2341 aggcagccct ggtcctgtac tttcccggat aaacttcttc cacaacctcc tgctttttga
     2401 gactccggag catctcggca accaactgtt tttcagccgg atccgtaatg ttcaaacgtt
     2461 tggcgacctg tttatagttt agaccctgtc tgttatttcc tgataaaact gtaagaatct
     2521 tttccctgat ccattcggcg gatatgcctg atttcctctt ccccttaccc ttcctgctca
     2581 taaaaaatat ttctgcgaag ttacaaaaag gattattaac tgactttcag aactgaaagt
     2641 tttaaccggt tttgcctgtt gtaagggttt taattcaaga atgtcctgaa tcatgacgga
     2701 ctagcacgtt cctgataagg atataaaagg agtaaacttt aaagtttaac gatctgttga
     2761 ttggaatact aagtttaaaa tttaatacat ttgaaatgtt aatgctgaaa tatgataaat
     2821 ctgaaatctg ttccccggcg gtctgaatca atagtaacaa gaaaaacagg caatgaatac
     2881 gttcttgtcc cggtaacgga caatattgcc gatatgaaca gtgtttacac cctgaatgat
     2941 tccggtgcct ttatctggga actcatcgac gggaagagag acattgagga aatgatcgag
     3001 gcacttgtaa gcaggtatga cactgacagg gaaacagcaa gtgaagattt gctttcattt
     3061 atcggtaaga tgagtgagta cctcgttata gacagatgat ccctgcatga aagtcacggg
     3121 gaagcagctg gaaacataca aaacaatccg tttaaactga ttttatattt tgaaaagcta
     3181 cgtactgaat attgcagact ataagatcag atttgaaaat cccgagggca acattgacct
     3241 gatgccttcc ggaagataca gcgggttttt atccgctacg gaaaaccatg atgtctgtat
     3301 cagggtgttt ccgggtgata cggaattgcc ggccggtaca caaaaagtct tttccgcacc
     3361 atatgttgaa gagatcaatg gtctcaggct aaaaaagaag gacaggttct ggagtgtcca
     3421 taaaaaagac gacactcttt atatccttac cgaattccct ttctccgggg ataacagaac
     3481 gggattgctc aggttctccc tggaaagcag gaactgggat ctttttctga acagcagttc
     3541 catccggacc ggttcttcag atcaggtttt tggcgctgat ccaatggatt atctgccaga
     3601 ccggggctgc cgcgcagagc cggcagacgg agctgtttcg cctcctcagg ctgatccaat
     3661 ggattatccg cttgacggac tgatcctgta ttatcttacg gtaatccatg gcgacatcat
     3721 gattcatgct tccggaatca attccggggg aaagggattc atgttcagcg gtgtatcagg
     3781 aaaaggaaag tcaaccatgg ccggattatg ggacaggcac ggagcacggg tcattcacga
     3841 tgacaggctg atcatcagaa aggtccgcgg ctcctattca atgttcaata ctcccgttta
     3901 cagaaatgat gaagccaggg aatctcctct ggatgccata ttcatcattg aacacggaac
     3961 ggaaaacgaa gccctgcggc tgcatggggc ttcggcgata acatccttat tgtcaaactg
     4021 tatacagcat aactgggacg aagcagtaat caggcagtct ctcgatgcgg caataaggat
     4081 atgtgcctcc attcctgttt ttcaactccg gttcaggccg gataacagta tcattgccta
     4141 tctccttcag caggtaaaaa caaatgaatc aggctgaaaa agagcacggg aagcatatgg
     4201 aaaagagcgg taatgaccat aaaattgcaa gggatcttgg cttttcgctt ctctcggaag
     4261 gcaggattct caggataagg gccggcggct acagcatgta cccatccata aagcccggaa
     4321 ccaccattct catcagtccg aaggagaata tgaaggatat tgttcccgga gatatcattg
     4381 catggaaaag agcacaggga tttgttgttc accgtctgat aagaacagaa gaaaggggga
     4441 atgaaattct ttattacaca aggggtgaca gttgcagaca tgaagacagt cctgtaacgg
     4501 gtgaacagat agcagggaag gtaatccgga cagaagacag gaagaacagg ataaaaaagg
     4561 gagatcagct taggaaaaaa ccggcttatc cgcttaaccg gataattgta aggtttataa
     4621 gcggaatata acatcttaca aataatctgt ctgctatctt tgcaaaagca tcagggtcta
     4681 atgaataaca aattcaaata tttccgggaa tcgctgaaac tggtctggca aagtgctccg
     4741 ggctggacct ttgccaacat aatgctttca gtcctgagga gtatcctgcc ccttcttctc
     4801 atctggctgc tgaaaaacct cgttgatgag ctcacgaagg ctgtgtcgct gaattcccct
     4861 gaagtcctgc cgtcaatcat atggctcatt gccgcagttg taattatctg gtttgttgat
     4921 gaagccacgt cggacctggg gaattatgtc cgcaagaaac agtcgatgaa actggaaatc
     4981 tacatgtacg gactcatcca ttcgaagtcc gtgaggctcg atctgctgaa tttcgagtat
     5041 tccgcttatt tcgattctct ttcaagagct tccagggaag ctccctggag accgaacagc
     5101 atactcaaca atatcgtatc gctgttccgg agcctacttt cccttctgct gatggcagga
     5161 gtgctggttg tccttcactg ggccgtggca ctgctgcttg ttgtggtaaa cattccggga
     5221 atatggctca ggctgaagta tgccggcact ctctacaatt tccagcgtga acagacccct
     5281 gaagccagga aggctgccta tttcaactgg ctcctaacgg gtgaccgccc gtcaagggaa
     5341 ataaggcttt tcggcctggg aaactatttt accgcattgt tcaaaaagtc ctttctcaga
     5401 acaaaggagg aagagataag gataatcagg aaaagaacgg ctattgaact ggtatccgac
     5461 ctggtcaagg cagctgccgt gatgattacc ctggtattca tggcaaggga aacgaccgga
     5521 ggaaaactca cactgggaca catgaccatg ttcgtcctgg cattccggca ggggatgatg
     5581 tacatcaggg aacttctcgg ttcacttgcc ggtctgtatg aagattcact cttcataggg
     5641 gatatttttg aattcctgaa tcttgaagaa aaaattaaag ccattccgcc tgtcgctgat
     5701 atccgggaat tcagctccgg tatcagtgtg aacaacatct ccttcacata cccggggaat
     5761 aactcccaca caatcaagga tgtatccttc agcatcagga aaggagaggt ggtagctgtt
     5821 gtcggaccga acggtgcagg gaaaacgact ctggtcagac tgctttgcag attatatgat
     5881 ccggattctg gatcgataag cattgacgga aaggatctga aaaaccttga tcccggtcag
     5941 tacaggaaac ttctttcagt ggtcttccag gatttcatgc tctataatct gggtgcggga
     6001 gaaaacatcc gccttggaaa catttcgaaa tcagatgctc cggaagacat cataaaggct
     6061 gccaggatga ccggtattca tgacctgata aatgatcttc cggatgggta ttccacggtg
     6121 ataggaaatc ttttcagcga cagccgggaa ctgagctggg gggaatggca gaagcttgct
     6181 atttcaaggg ctcttttcag ggatgcgccc ctgcttattc ttgatgaacc ctccagttcc
     6241 cttgacgcgg ataccgaata tgaaatattc agccgcttca gagagatagt aagcggacgg
     6301 acatcaatac ttataagtca caggtttata aatgtgagtc ttgccgaccg gataatagta
     6361 ctcagtaagg gaagtatcgc ggaaagcgga actcatgatg aactgatgga gaaaaaaggc
     6421 atataccaca gcatgtacac aaaacagaca agcagattcc ggagatgaca gaatccctga
     6481 acctgagaac ggaagaacag ctgttatgcg gactttgccg gctgtcattc agcagtgcgc
     6541 agactgaaaa gcttaaaagc tgcatcggga atacaaatga ctgggattat ttcctgtcac
     6601 tggcacgaaa ccatggggtt tcagcgctgg tataccacaa cagcaacaga cttggcctga
     6661 ctgatcttat ccctcctgct gtgacaggca gcctgcggaa ttcatacatg atcaatctgt
     6721 caaggaattc gggttttctt gttaaaatgc cccctgtcct taaacttctg aacagccgga
     6781 atataaaaac ggtcctgctc aaggggcttg cactggaatt aacggtttac ggaaattccg
     6841 gagtcagaca gatgaccgat gttgacatac ttgtttcacc ggagaatgcc ctggcagcca
     6901 ggcaggtcct tattgaaaac ggattcgtgt caaagcccct gaaatctgta ttgtacaagc
     6961 cgcttctcac ctgttcagga aagcatctgc cgagtctgtc agctgaaggt ctttcggttg
     7021 agatccatac cggacttttc ggaaaaaaaa attcctgcct cacccgcaag ctttacgatg
     7081 agagtcctga aaaggatctg ggcggtgaaa aggcatacat tccttccccc cttatgttct
     7141 ttgcctatct ggtaaggcat ctctggcttc acgagatgaa aaatgaatcc cagctaaggc
     7201 tttatgccga tctggtggtt ctgatagaaa aatatcacgg ggagattctc ggaccctcgc
     7261 ttacgggact ttgcgaaaag gccggaatga atgagatcat ggcaaccagg ctcagacctt
     7321 tgagagatgt tcttgaaatc tcctttcctg atgacatcaa taatttcatt gacgaatggg
     7381 ctgctccgga ttcactggat aaattcctgt ttttcctgaa aagtcccggg aataatcctc
     7441 ctgtaagcag ggcccggctt taccgttacc atctgggtga aatacccgga tttcaccgta
     7501 aactcctgtt tatcgccggt gaccttttcc cttcctttga atttatgaaa aacaggtacg
     7561 cctgcaggag caggctggaa actcttatgt actatcccct gagactgggg aaactgtggt
     7621 acctgatcgg ttagaaagtc ccatgcctgt ctttgcggcc ggcgggacag tttcctgcag
     7681 aaaatctaaa aattctgcca ttacaaggta acaaatcagc tgctgtgcat attaaacagt
     7741 aaatctgaaa ccggcttacg ggctttttcc tctatcaaag ccggacaagt ggaataaata
     7801 tgttgtcagg taatgaaata ttgtttttcc ctgagaattt ttcgaatata aaaatcgaat
     7861 tttttaattt ttttccgaat attgacatgc tcctgattta tttatgtaaa tttagaattg
     7921 aaaggcaatg tcatattatt cgaaaacatt aataatatga caaaaaagaa aagagaactg
     7981 acaaaagaca gcatcctgaa cctgatcaga ttaagggctg ctggcactcc ggcagatctg
     8041 gccggcagat tcggcgtaag cgaaagaagt ataaagcgga tgataagcga attaaagctt
     8101 gagggttacg ggatagtatt ctggcatgga gggaatacct atatccttga aaatgatatg
     8161 gatcaggata taaaacgagc tgtctgaaaa aaccctttgt ccctgaagga aaacaatctg
     8221 tttcgtgcgg acaggaatat agaaaacccc ggattgtcag tgccttgatg ggatggtaac
     8281 tttcaatccg gggatatcag gtgtgtcgtg gacgaaacac ctgtctttaa gcacaatttg
     8341 tgcagcgaga tttcgtccgg tccgcacatc ttcatgccgg acccgtagca cgttaatatt
     8401 accctacaaa aaccaattta aatccacccg gggacagaca agccgtttga atgaactatt
     8461 tattcacacg ttatcaacag gttattttat tgtatttcag tatgttatct gttttttgtt
     8521 aataacttat taacttcctg ttttacattt acttatcagg cggtatttgc aaaacacggc
     8581 actgtttcaa tggcttattg tttagctttt caggaaagga tttcaccccg ggtccgggtt
     8641 attatcatgc agcctgcttc gaattatacc tgttttgtaa attcacaaat actgaacata
     8701 taccatcccg ttgtcaggta aaacagtgat gcggccagta tcagccacgg aagcggcttt
     8761 gcagacggaa tcagcaaggc aaacaagaca ataattgcac tgacggttat aacaatgaca
     8821 ggaaccaggg actttttatt ttccataata tttagaataa ctgatcaata gcacgatcca
     8881 gggccttaaa tcacagtaat gccctatttg gagaaatctc ccggcagtta tttcaaaata
     8941 cgcttcaggc aatgatccgg aaagaatatt cgtaaatttt cccctgttcc ggaattcatt
     9001 tcccttgcag cctgctgtct gcagcaggca gggaacggac acaaagagat ttactgagac
     9061 cattcactgg gctcacggtc ccagcgatgc tttctgagtt ccttcatcag ggcatcatcg
     9121 aggggtcccg catcactggt tgcgatattg gaccttacat gagattcctt gcgcatgccc
     9181 gggattatcg tgctgatagc gggttcccct aaaataaacc tcagagccat ttccggcatg
     9241 gtcattcctt ccggcaccag gggtttaagg gcatcagcat gttccacact ggaatccagg
     9301 ttttccttta tgaaatatga attcctccag tcacctttcg gccaggtgct ttcttttgtc
     9361 agggtaccgg taagtgttcc ttcatcaaac ggaacccttg ctataaccgc aacattcatt
     9421 tcacgacagg cgggaagcag ttcatcttca ggattctgat caaatatgtt atatattacc
     9481 tgaacggaat caatcaggcc gctcctgact gccctgattc cgttccaggg ttcccagcgg
     9541 ttaaggctta tgccaatggc atggaacaaa ccctgttcct tcagttccat catctttttc
     9601 aatccccttt catcttcaag ccagccatcc tcccaggtat gaaactgcat cagatcgtag
     9661 gaatccagtc ccagattctt aaggctgcgc tcaacaaatt cctgaatatg atccggcggg
     9721 aaacagtctt caagtgtata ttcccttctg gccggccatt taaaattttt cggcggcatt
     9781 ttggttgcag tgtacagctt cttgtccttg tttgccctta caagctgtcc tatcagatct
     9841 tcactcttac cccggccgta agcccaggca gcatcgaaga aattgcatcc gaggtcagcc
     9901 gcaagctgaa gggatctcat tgattcctca tcatctgaac cggtccatga tcccataccc
     9961 caggtgccat agcctatctc actgattttc catcctgttc ttccaaatgt tctgtatttc
    10021 atatcttttt taattaattc ctgtaaatca gattattcct ttatgcattc ttgggattgt
    10081 cgtttccccg gtcagatttc cgttcaggct gcagctgaaa gccggaaaac gaactctatt
    10141 tctctgcaag catatctctg agcttatctg tttttgaaac atcaaagcct gatcccgaga
    10201 gatatccgat tattccgcca taatgacctt caatcctgtc caaaaccgta acgatatcct
    10261 cctttttctg agttacggca tgaagcacag aatcagccgg aaaaaaccca agtgtaatga
    10321 attttctgac tctcagcctt ttttcataat gaggtaccag tgaatcgttt gaagccatat
    10381 aattcctgat gatctcgtcc ctgtcggcgc cggcaatcat ctggaacaat gcgctcagta
    10441 ttccggtcct gtctttccct gcctggcagt gaataagtac aggggtgctt ccgggttcaa
    10501 gcatcagttc tgctgttttt ccaaggaccg gccttacagc atccacaatg tcaacataca
    10561 gtccgttgat caccttgttg attgctccgg catcataacg gcgtttcatg aggggcacaa
    10621 gcctctcccg ggttacactt tcaaaactca atggatagtt tattatcctg attccgggca
    10681 gttctgcttt tcttcttgaa aattccgccg ggtcacgcag gtctgcaact gtttttatcc
    10741 cgagggcctg cagttttttt aaatcccgtc tgcttatcct gtccggactg gccgacctgt
    10801 aaataagacc cttcctcatt ctgccgccgc cggtaagatt caggccgcct gcatccctga
    10861 aatttaaaac tgatcttatg tctgtatctg tagctttatt cttcattcct gtaaaaataa
    10921 ggaatattgc cacaaaatct caacctgaaa tattaaaagt cagccggcga aatatgaaat
    10981 ccccgataat actgcagagc agcactagtc cacatacaaa ttgttgataa atcatgcttt
    11041 ttaaacatgc cggtttgata attcagcttt gccaattatt gaatcggttt taactttacc
    11101 cccgtctgat atcagtgttt aatattagta aggttaactg atccatcacg gatttaagta
    11161 ttaattgaaa taaaccattt attatgaaga aaatcggatt attatttctg acagcaatat
    11221 tgattatcac tgtttcctgc agcaagtcgg atggtgatac gggtatcctt gaattcaagg
    11281 gactgaaggc cttccaggaa acatctcata aatcatctga gaaaagtgcc agggcggcat
    11341 ttgccgatgg accgcagacc cataaaatgc atactgccga tatgaagttt gatattgctg
    11401 agatatgggt ttcacaggac atggtagaag ataatattaa agataatctg acctggcacc
    11461 ttattggcag gaacaagggg attaaatcct gtgaagatta ctcgttcagt gccagcaaac
    11521 tgcctgttgg ggaatacaaa agtattaaaa tggtgttcag aaaccggatc atccgcattg
    11581 cagtttatgc ggacaatttt agtaaatctg tggaaatgaa cagttccctt gatgaggaat
    11641 cggaagggga tgatagcttt attacagaat atttctgtcc aaacggcagt tttatacttt
    11701 ccggtggtat gattaaactt atgtccacag gtgagaaaat aagcggattt accataaggt
    11761 caggagagaa gacaattttg tcatggaaac tgggaggtcc tgacagtaaa attacggatt
    11821 gttcatttga ctgggttgat accaacggca acggtcaatg ggagtgggat tcggatgaaa
    11881 tcacaaacga tcattgctct gtggaaatgc ccatgttttc gttcattgtc gaggaatgat
    11941 tcattcccct gccaggggag ttactgtttg cattagtctt tattttcagc ttttccctcc
    12001 tggattgtca ggagggaaaa gtatttaaaa agcttttata attcgacctt tcagggataa
    12061 tttacaatca gatcccgggt aatatttccg cagcctgaaa cagataatgt atgcagggat
    12121 gcggaaaatt ctttttatat cttgctataa atgaacgtgc tgtaaaaacc gaaatggtga
    12181 aatacaacag aaatatcacc gggagaaggg actgaaaata caggtagcag actgaggctg
    12241 ccagtacagg aatcatagaa aagctgttta acatggggcg gttattggaa gatgaaaatg
    12301 ctgctttaac cggatttggc cagaatgtac taagatgcaa tgaaaccgga atgagcactg
    12361 tcagcactga aaaataaaag agcagccatg cctgcaaatc atacctgccg taaaaaataa
    12421 aagcaaaaga cagcaggaag tcaaggagaa tcagcggaag aacagtttta agcaaaagtc
    12481 cggtcaggga atcatattct gtaccggaaa ttttaaaatt ggtaatgaaa gccgggaaga
    12541 aaccgaaaaa attattgaat aaatatgtaa aaaccagaac cggagaactg taaaaaacta
    12601 ccgggatctt aaaggcagtg ttcgcatcag gatcaatttc caggagcagg aaaaagaaga
    12661 gaattataat tattttaacg gcaaacatta tcagaacaga aacacggaag attttattcc
    12721 tgatgtacaa ctgaattaca aacggcagat gcgatccgcg atcagatatc ctcctgtcat
    12781 aagtccactt attaagaaca agctgtagtc caaaaccggc aacaactgtc aaaacattga
    12841 aaattacaga atcaatgtat ctgaatatga aaaacagaaa aaccgtgtaa attatcacca
    12901 ttaaaagcca gcccggtatt ctgtggatgc ttttatagaa gtctttttca acaagaaacc
    12961 ttagattcct gcttaaaata acagcgttga gcactcctgt caataagcag cccgcataac
    13021 gcatcctgcc gggaaaggga actttaaaga acaaggccga aatcaggata acagccgcga
    13081 gtataataat gaaccgcgac atacaatcac tgataaggcc ggtcagggct cttgtaaaat
    13141 tatttatggg ataaattttg ctgaagggtt ctttacatga tttatattca ggaaagaaaa
    13201 aggatattaa agccaggatt gaagcacccg aatatagaaa gacaaaaata tcctcccact
    13261 gtattattcc cctgtctgcc agcctgtcca tataattact caagaaaaag gcataaagca
    13321 gaaaagcagg aaatgcaaga aagaaaatca gtttttgtct gcgtcttcca caattccgga
    13381 acaggagctt aaaccggact tttatgaaat aaaaaatgaa attcatttcg gcaatttatt
    13441 ccgtttccgg cagaatctta tttcccccgc caatcttctg aaatccggga tctgcatgaa
    13501 gatccgtgat tctcccattt gacaatacag ctatattatt tgtaagctgc tgtaaatgac
    13561 ctgtctgacc ggaggtaatg ataattgttt tttccgggga aatgtatgct ttcagcaatg
    13621 ttatgagttt ccgacaggct gtggcatcga ggttttcaaa aggttcatcc agcaataata
    13681 attgtggctt gtgaaaaatt gcagtgcata ttgacatttt cttcctggta cctgtggaat
    13741 atgaccctat cggtttatac agaacatcag aaaactcaaa aatgtcgctt aattccagaa
    13801 ttcttttttc agtctcaaat aaggggatct tatatatccg gcctatgaaa ttcagaaatg
    13861 tataaccgtc aagttcatta atcagctggc tggtttcata taaagatacc ccgatcacgg
    13921 gttttccggt tgtattgttt ataatgcgaa catttcctga gtctggtttt tccaggccgc
    13981 atattatttt cagcaggctt gttttcccgg aaccgttctt acctgtcagt ccaaaaatct
    14041 gacctctttc aactgataaa tccagattta ttaatacttt cttgtctttg taacttttat
    14101 atactttctg aagtacaatc atttacaggc aattctaaaa aacagcaatt attgattatt
    14161 tgtctgatac ctgaaataat cctcaacctc tgacattgat tctctgttgt tttgaagatc
    14221 tcttattatt ttaccctttt ccatcaaagc aacacgggtg catatatcag atatgtgatt
    14281 cagattatgg ctggaaagaa taagcgtttt gtttttatcc cggaatcccg aattcagtat
    14341 cttatttaat acgatctgcg aactgggatc caggaaatta aaaggttcat ccagtatcag
    14401 tacttcagga tccgggagaa gagcgccgat aacacctacc ttatgcttct ccccggatga
    14461 taactgtctg atatatttgg aagtttctga tatttcggaa ttaagaaaat gattatagcc
    14521 ctgcagcctc tcgtaatatt catttttttc taaatcatag atttttgcaa tgaactcaaa
    14581 atattcctta ggtgtcagga actcgataag aaaattatta tcaataaaag tgcccgtata
    14641 atttttccaa tgttccgact ggtaaaccgg aatcgatttt gaaagtatct gacccctgtc
    14701 aggtttcagc agatcaacca ttaatcttaa gaaagttgtt ttacctgccc cgttatttcc
    14761 aacaagcccc catatatccc cgttttcaat tgataattcc ggaatatcca cggcaatcgt
    14821 gtttccgaat ttttttaaaa gattctctgc tttgatcatt tcctgtatcc ttcaagattt
    14881 atatacttcc tccgcattaa tttattttga ataattttcc agagcttacc ctgaatgatt
    14941 aaggcaataa tacataataa gttaataata aggaggctat gagttaaatt tagccggagc
    15001 agcaaaagaa acagggtcag aattggtatc agcagaacta acaatacccc tattacctgg
    15061 aaactgttta atccctgata attaaacaaa atacttcggt tcggatcaac tttgaattta
    15121 ttataggttg ccaggaaaat aagaacaaag tatccaaggc tggaattata caataatgcc
    15181 gagaaaaaca tatgtaatcc ggtctgaacc ggttttatca caggaaaata aactgtgaat
    15241 agaattacac taaaaattga atatactgca tattttgcct ttaaatattt aatgatatcg
    15301 aattttgtcg ataagataaa atcaaagtat ccgctttccc atgaaaataa aaactgaata
    15361 aaagtatagc caaacatccc gcttataaga atgaaaagta tgaaaataac atacagattg
    15421 ccatcataag ggagaattct gtaaaacata attgagaaga ataaaattat cagcaaaaaa
    15481 cctcttgtcc gcctgtttct tgcaataagc cggatttcca gaaggacata tgtaaataaa
    15541 tcattttctc taaaataatg actggcaggt ctgaatgaat aattcaacaa ggatttttga
    15601 ttttgataaa taccataaaa gccctttttc aataaaatga aattgcagat taataatcct
    15661 gcaagtatta aaatcagtaa tatcagaaaa tatagatttt tgttaatgag ttgcttaaag
    15721 atgaaacggg acatgtcttt aaaagaaata tccaggaaaa gatccattaa gtaaaggcct
    15781 ccaaacagaa gtatgggtat aattatataa ctataagaga tcccgattaa attcctgatc
    15841 agcaaactga aatacccgac cgaaaaaaat attattaaaa ctccgggaaa atagaagaaa
    15901 aactgctgga tactgtaaac cggaagaatc gtcctgattg aatatggaac aaaaaacaat
    15961 aacaatccga taaaaaaata attgaactgt gacagaaaaa gtatatacaa taccagcttg
    16021 tttcttttaa tcggtaagtg taaataagcc agcagtgctt tcagtggatt tttaatagaa
    16081 aaaacctgca ggataaacag aaccgcacct atatgtaata acctgttatt aaggaatgta
    16141 accggatcag atgaagggct gtacaagtat aagaaaccag aaaaaccata acccagatag
    16201 accaggaaag aaaaaagata taatatggag aggatcataa gcaacctcaa tattaatctc
    16261 aggtcaaatg tggtatttct gagaaagctt tttattgagt gctttataac ctctgttagc
    16321 atgacttgtt ttacattaaa aagacaaaca ccttaacggt ttgccgaaaa ataccaggta
    16381 tccccctcca tgatgaaaat cgtttctgca atctcatgca gcagaaaaat atatttgccg
    16441 gatctgcaaa cggaattccc gatgatacca gtgccccata cccatgcaag taagagcttc
    16501 ccttcgtgaa tgaaagttaa atcaatcact tacaaagagg ggttctgttt gattcaatcc
    16561 aaactccttc ctaagaacag caaagaaact aatttctctc tacaggtttt cgtttcctga
    16621 ttacagatct aatatacaca attacatctg tatccggaaa atacaaaata ccagtccggt
    16681 gctgccggat taataaaatt caagggcccg gagccttttt tcagaagatc ctgaaaatat
    16741 gttctgccat atgatattct gtcagaaaga acagtgaatg aaatcaatta agcggaaaaa
    16801 agagtcggga aaatattacg ttcatttttt tctaagtcag actttatttt caggataaat
    16861 aaaactgata gaaatatata aaacggaata agcatataaa accatgagtc aattttcatc
    16921 agatagagta aacctgtacc aagcagacaa ataaaattgc ctttcgggga atgagctatt
    16981 ttgaaaactg cagaatcggt ggcataagca ggactgacaa atgaccagta gaaagaaaag
    17041 cagcttaaga aaaacagaga aaccaaataa aaggatataa cgaataattt atcattccat
    17101 agtattaaaa gaaataccaa tgaaataacc atatctataa caagcggtat tgaaatcagt
    17161 ttataaaaaa tattcagata ttctttccag tcattccctg aatgaatcct taattgcagc
    17221 cacaaaatac gataatggcc ccaaaggtta ttaaacacat atgagaaatg aatagtggga
    17281 gcagctatta aaatccagta taataaattg ccgtctaaaa tatgacgtcc tgtatgcctg
    17341 acaaaaaaca tatccgtgat aaaaaagacg atcttcaggg ttaatccaat aatcaaagac
    17401 aatctgacgg ccctgttata aaataaaaat tgccagaaaa tattcttcac agtggatttc
    17461 ttcagatgca gaaagcttct gttttgtccc ggggttttac tctctataaa aaacagaaga
    17521 aaaactgaaa gccccgaaat catcagtgcc cagtcaaagt tcgacggcat ataagggcgt
    17581 ttgaaaaacg aaattgaaaa aaccaggata atcagtaaag ccatcgttac agatgacctt
    17641 aaatcatatc tgttctcaat aatattctga aatatcctcc ggaaaagatg tgacgtaata
    17701 ataagtaaat aaccctgtat taaaagcttt aaattaaaat ctttggaaat aatggttatt
    17761 gtcagaagaa aaataaacaa gaaaatgaag aaacccgata acatgtcaat aaaaagattt
    17821 ataatcattt tattgaaact tgaaacagga tgataatgta gtactatcct tctcagcggc
    17881 ttatatgccg gcaatacaat tcttatgaat ggaagcatga aaagtcccgc taataaatat
    17941 actattgact catctgagcc ttttaccagt aatccatcat ttgctgtatt aagtaaataa
    18001 cctgccagaa gaccaagtaa tactgaagaa gagaaaaaga caattatttt atataattgc
    18061 ctggatttgt ataaatcaga gaagttcctc aataacaatt taaaaatctg ccacattatt
    18121 tttcagttta gccggaacgg atttattcag tttcagttta aatttcagtt tcaatttcag
    18181 tttcaattaa cctgcaaaac gcatcttcaa aggactctcc ttcccgaacg ggaaaatcat
    18241 tgattttaga atctaaaaag agtctctttt tatggacaat ccctatatgg gttgaaatct
    18301 gttttatata attcaggtta tgtgaggaca gcaaaatcaa tctttcagga ctgatataat
    18361 ttttcagata agtaacgagt cttttagcag aaaccgggtc cagaccagca aatggttcat
    18421 ccaaaattaa cagatcaggt tcatttatta gtgctgcaat aattgcaact ttctttttca
    18481 tgccaaatga atacgtccca atcctctttt taagctctac ttccttttca tcaaaaaaat
    18541 aatctgccag ctcgctgatt ttatgatctg ctgctattcc tgacatattg tagaaatgac
    18601 cggtcagagt aagataatca aatccggtta attcatttat gactgccaga tcttccctta
    18661 aaaccccaat tcttgcctta ctttgattgt caagtttatt accgggtttg ttcaggactt
    18721 ctatcagacc actgtctggt gtgagtaaat ccaggataag attcagtata gttgttttac
    18781 cggctccgtt cctgcctaaa aggcagtaaa cctgagctct ctccaggctg agatttatat
    18841 cctccagtac tgaattacca ttgaatgatt tattaacaga cttaaggctt atcatattaa
    18901 ataattattt tgtcaaacac ataagttcca cttattcagt cattatggag cttatgtgca
    18961 gaatacaacc tcggaaacaa aatcactaat aaacttaaat catttgtctc gttaaacaaa
    19021 gaaaggataa gtgtatcccg gtcatagtca aaaaaaggga ttggcaaatc ctgcaaataa
    19081 acttgctacc acacagccta attaagcaac ttgtgcttag gcatctttac gggtctcatt
    19141 tcataaaaat tttcggcatc gattcttttc ataatgcaaa tagcttcagc aatcttcgaa
    19201 cagacaatta taacgacttc gcaaaggcta aaatatattg ttcccgggtc tgctgacaat
    19261 atcccacttg ccaattatct ttcatatctt ttctgccact aatttgatag tgaagcatat
    19321 tttcataacc gattgttttt ccatagtttc accataaaga tgtttactct cttcaaattt
    19381 cctggtttat acgaacagat ttaatatata gtattttatc tgtaacgaaa aagaggagtt
    19441 attaattgat aacaagtagt cataaaaatg aagtatttgg cgctcaaaga ttgactcatt
    19501 acctccttgc aaatgattga tttttgcaat gcatgaaaac cttattcaaa ccagtccata
    19561 ttaatacttc taatattttg tttcaggata ctgtctgtga ttaggctttt tatttcattg
    19621 tgggacatat tttcatgcaa agcaatgtca ttaataattt gaccatgatc cataatcaga
    19681 aatcggctac ataaattagt cataaggtct atattatgag aggtaataaa taaggtcttt
    19741 ttatttctta aagatttgat taaggatgag atgaagtcga tagtttggaa atcaagatga
    19801 ataaatggtt catcaaacac cagaacttca ggattatgta taatagcagc agctaatgct
    19861 attttcattt tatctccaga cgaaaagtct ccgatctttt tcttgttgaa agaatttaag
    19921 ttaaatgatt taatcaagct ttctattcta aggtctattt caaattgatc aactttttga
    19981 aatttgcaga caaattttaa atattgaata ggagtaaatt catttacaaa aatcggggga
    20041 ctcaacaaaa tgcctagttt atttttatag gtgacaaaac caggaattat tttattacca
    20101 taaattaaaa cattaccact atcataattg actaggttac ataagacatt tattgtggta
    20161 gttttaccac atccattgtt accaatcaat ccaactgatt ctcctttttt aatagagaat
    20221 gacaagccat tgagtgctgt tattttccca tatcttttaa taactgaatt aaaattaatt
    20281 atttcttcca tggctaacct ctctcttcac ggctcaattt aattaccaaa aaaataaaat
    20341 aggatattaa tatccaaaag attattaaca tcaagaacaa tcctgacaaa atgtaactta
    20401 acaagatcgt gaacgaaaaa aaagtaaaat aaaatattct gagcagtcta aggtgctcat
    20461 caacaaatat caactttgtt aaaaaaaaca gaagtattat tgaaattatg gttagatata
    20521 attgagattc tattttgaat ttaa
//
LOCUS       DAOSPX010000018        20275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2821_length_20275_cov_4.717804, whole genome shotgun sequence.
ACCESSION   DAOSPX010000018 DAOSPX010000000
VERSION     DAOSPX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_20275_cov_4.717804"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLZ75_01890"
     CDS             <1..941
                     /locus_tag="PLZ75_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS71181.1"
                     /translation="PYQEAKTDTTKSWCVGGLWAMTEYPVNTPGAKELLQKAKVQLDF
                     LYGKYPIGAFTDLNNPAMDNKGGAIFQVQYLVGTSGNGCIPCFVPLSSHTTVLSIETG
                     TIIPSLAYFNSYDPADKRIVDRNYFYYSDTYAAKYDVNEGPAGKFPLPFLYKYYDYEC
                     MKVKGESGLNFNLYRYADILLMLTEVNWALTQLGVSVPSADVVKGINEVRERAGLSTF
                     ASAGDLTLKDIMSERAYELIMENKMLWDMRRTRKALVDGAGSFIRIENLVGHQPTSFN
                     YKFSAKHLLSPMSVNEIANNVKVLQNYGWLPVQIGQ"
     gene            977..2149
                     /locus_tag="PLZ75_01895"
     CDS             977..2149
                     /locus_tag="PLZ75_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71182.1"
                     /translation="MKKLIFFTLIMMLFVSCQDELYDIPRDAGGKAILTDISSTATTG
                     ISTLDDQFSVTAYLPNAKSGDIMNVECLQLQVPAGATTTQLLPLPGTQKTATVGSDLK
                     ATITFTRTEAKLNKAGDYVVVTFGGETESAKQRVDMVPATTTTKPRVAGKDVDVARTD
                     ETAFFNVSVVPKIGTFTDTLTAKRKNGINDVLTDVPGSPFSGTQPFLVPISGNDFAPD
                     KDTMYYVFMATSGSYTDIIEYSVVVRDPYFYLKKTGVTLNLGGSSAGRNLLRNAAVAE
                     DNANATIAVSGSLILKGGSAWLAAGNSIEFVPATAALYSTNSTTASIAAFSAGTPSET
                     ADPIAGEGIFIFKIVNGPDPENVIYGMIKVTSVIPGVSVTFEYRIGNLYAHLAVIS"
     gene            2325..5225
                     /locus_tag="PLZ75_01900"
     CDS             2325..5225
                     /locus_tag="PLZ75_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOS71183.1"
                     /translation="MKKIHVRFIMILAGLMISVIIADCQTISPSGIEFKNAPVLFKEG
                     GKSFQQILVNYRAVNPEKLVITSSGKELLNANLTKGVNRFLLTLPAVSRPGKITLTAR
                     IGNGPAEKCPFTIVPPKKWKIYFVQHSHTDIGYTRPQSEILAEHMRYIDYALDYCDQT
                     DNLPDDAKFRWTCESAWVTREYLRSRPSEQIRRLKERVTEKRVEVTGMFSNMAEIADE
                     NVMYDFLQPVKEISSHGINVETAMQNDVNGIAWCMPDYFKNTGVRYLIMGINMTRSIL
                     PFDKPTAFWWESPSGARLLAFRADHYMTGNNFGIESRELKAENMLWHIADLDDRGYPF
                     DRIGIQFSGYRTDNAPPSTAACELVKQWNAKYEYPKIRLAVAGEFMEYVDRNYSGKLP
                     VYRNAWLDWWTDGYGSTSRETAEIRKTQNIKQVDEGLLAMVTIMGGEMNNAIEGKLNH
                     INENAIFFDEHTCGADESISHPYSENSTKQWLQKGAYAWEALKRVTLLNEEALARFQP
                     FLSKASFPVIYVINPMGWDRSGNIELFVDNEVVPVENKVNIIDISTGRKVPAQVKTRR
                     REGSWWIIEVNNVPAMGYKALKVEIIPEPATIQAESEKTEILENQYYRLVIDKTSGSV
                     SSLFDKALDQDLVDPENQYHLGQPVRETSERRDVPPFIRTSVANVRVDKGINGDVWES
                     VRISYDLDGFEKGSPGKPGGIQTEIRLYRNVRKIEFRYTANKLILTDPEALYVAFPFA
                     LKDSRIVFETIGGILTQGEQLPGSSSDWNVAQNFVAVRGKSGQVIIVSNEVPLWHFSD
                     FNMGKFERYPVKGKPWLYSWVMNNYWFTNFRAYQEGAFSWGYQLTSSGDTSNTFATHW
                     AWGERNPFLTRTFPAGTGELKIPSFQTLKLSGTGNVLPVNSRPVFNRERTILLHLREL
                     EGLPGEVEISSAAPGQMIRKMTEVNSFGKPAARLSSVIPLKPYEVKFVELEF"
     gene            5249..7507
                     /locus_tag="PLZ75_01905"
     CDS             5249..7507
                     /locus_tag="PLZ75_01905"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOS71184.1"
                     /translation="MSKVRLFLVFLLVPMACGNNDVTRFVDPNIGGVAPLLTTKCPTV
                     HRPHSMVRVFPVTKPGLSDRYLSDRIYGFGLNMPAYRMGHVTSIMPVPGELSFDPDAT
                     ASSYDHDLEDVHPWKHTVFLEDHDIVAEWTTSERAVVYRFVFRKDVPANVIFRSAGQA
                     SFRIRGNNTIRGWEEFRNTKQYFHAEFSLPFDSRGTFNGSEIIPGSDEADGRGTGACI
                     SFFAPGQPLEVRIGISYIDEDQARENLEREMKGKTFEKICDESYRIWKDALGKIYAEG
                     GTERQMRIFYTSLYRCFERMVNISEYGRYYSGYDRKVHNDEGKPFYVDDWLWDTFRSQ
                     HPLLYIIEPDKQLDMAQSYVRMYEQSGWMPTFPQFYGDFSAMIGFHSTALVWDIYQKG
                     GRDFDVEKAYEGFIKNAMKGTLVPWRSGPMCGLDSFYIQNGWFPALPEGAEETEPLVD
                     GFEKRQAVAVTLEHSYDDWCLAQLAKALGKNEDYEYFSRRAQNYRNVYNPETGFMAPK
                     MADGNWVEPFDPTLSGGIGSRMYFAENNAWIWNFSVMHDIPSLIELIGGAETFTDRLD
                     RLFNEPTGIAKWQFMGQFPDASGLNGMFPAGNEPSFHVPYLYNYAGKPWKTQRRIKEV
                     MELWFDDDPLGLPGDEDGGALCAWYVLSAMGFYPVTPGNGLYAIGTPFFKKVSINLPD
                     GKIFTIKARNISKKNKYIQSAKLNGKPFDRAWIRHSEITGGGTLEFVMGDRPNREWGN
                     DPAVLEQLTADN"
     gene            7534..10716
                     /locus_tag="PLZ75_01910"
     CDS             7534..10716
                     /locus_tag="PLZ75_01910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOS71185.1"
                     /translation="MTRFILALLFISFSLLSCSRDTKIVWSIGSEDGSSADLALGPDG
                     FRDFLSRDFGFEDKYFLIGHSVDRESFPYVLPGPADTWGGTWSTAGWRTHQINIFFGI
                     REMQGKSMHGKWKLVISLLDFSGRFPPLLKASINEYDRKIRLFAGEYQPADQVHPGMT
                     AAVTDTASISGDLKDIHPRKIEIPVDPGIIKRGGNCITLTILEGSWIMFDELHLEAPG
                     NVSLIVPEEVFIREVKASSYELDQGEDRIQPLLVTAVHLDGSPEISVELDGKEIFRER
                     TEAGICQFEVPMPATDKRIKSGYKIYSGNVLLEEGTVIRSPQKAGTLADYVDTRTGTA
                     HSRWMIAPGPWMPFSMVKLSPDNQNPGWQAGYEPSFESIGTFSHIHEWTLGGLGIFAS
                     NGRLITKIGDELLDEEGYRSGINKRTEEAPLGYYRADLTRYGIKAELTATTRCGFLRF
                     TFPSGRDSSRILVDLKVPAEYSYELKEINIKATGKNRIEGFCRQFAPRVWSNDAQQDY
                     TVHFVLETDRPFKSMGSWLNGKIIYSDSLYAKEAEDAGIFLEFDTRKQRAVQLRSGIS
                     YVSIPNASENLETEISGPFGWDFDAVRNNNVNTWNDIFGRVLIKTSDRLEKVRFYNNF
                     YRALCSRNIFSDVNGEWISTDGLTRKFENPDHVALGCDAFWNTFWNLNQFWNLVTPEW
                     SERWVNSQLAMYDANGWLAKGPAGMNYIPVMVAEHEIPLIVSAYQMGAGNFDADKALE
                     AAVKMQTTPAQKVFNGFAGNRDLIPWLRYKYVPYDKGRFSNSMEYSFDDWTVGQLARA
                     LGNEELYRKFNERGYWWRNAINPENGYCHMRHSDGTWLKDFDPFLSGANRHYVEANAW
                     QMTFFVPQDVPALVDAIGKDRFNERLQWGFEQSEKWRYNAPNDQYWNYPVVQGNQQSM
                     HFSFLFNWSGKPWLTQKWTRSIIDRYYGTGTANAYLGDEDQGQMSAWFLMASMGLFQT
                     DGGCRVSPVYELASPVFKRIEIDLGNRYGRGEKFIIEAENSSRKNMYIRKAILNGTEL
                     ESFLIPAAELLKGGSLMLEMGSEPDTTLFR"
     gene            10910..11584
                     /locus_tag="PLZ75_01915"
     CDS             10910..11584
                     /locus_tag="PLZ75_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOS71186.1"
                     /translation="MRRSEVNKIIMDFERFLAEHKFYLPVWAYWKPEEWKGKYDICSE
                     IVDNKLGWDITDFGSGDFKKTGLSLFTIRNGNWDKKDKMYCEKIMMADEEQETPMHYH
                     WAKTEDIINRGGGNLVMELYYATDDDRLSEDPVTVSIDGVLTTVEAGRPLILKPGQSI
                     SLKSKLYHRFYGEKGRGKVLIGEVSLVNDDAKDNKFHEEIGRFPEIDEDVSPHRLLVN
                     DYEKYL"
     gene            11621..12808
                     /locus_tag="PLZ75_01920"
     CDS             11621..12808
                     /locus_tag="PLZ75_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOS71187.1"
                     /translation="MKRKIIVSGTGCCLVDLLFNNIDFSSDQILDFLSKERGDGGLSP
                     GKLVFQEEFEKFSGKPLVSVLDTLTGGRKPDKINIGGPSIVSLIHLAQVTGREKCEVR
                     FHGSRGKDDIGEYLLTALKRTPVVLKDYRITERRTPSTVVLSDPQYDRGHGERMFINS
                     IGAAWDYGPDDLDSEFFGSDIVVFGGTALMPKIHDRLAGLLKKAKSAGAVTVVNTVFD
                     FRNEKRDPSGRWPLGESDESYSHTDVLITDMEEALRLSGKHDIESAAEFFIKKKVSSF
                     IITNGSGNITLYSDGRFFRPSEMAEMPVSQRIILEIKDVREGDTTGCGDNFAGGIIAS
                     VVEQMHDGIRHPDLREACSWGIVSGGYTCFYMGGTWFEERAGEKLERIRPYYESYREQ
                     TGH"
     gene            12756..13991
                     /locus_tag="PLZ75_01925"
     CDS             12756..13991
                     /locus_tag="PLZ75_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71188.1"
                     /translation="MKESDPTMNPTGNRPGIDDHSIVIFGAGRIGRSFIGQLFGRSGY
                     RLVFIDADPVLVGRLNAAGCYRVIMKGEKEEEIVVSNLEAIHAADTVHAAGAVSSAGI
                     LAVSVGMNAIEKVVPSVAAGIDLRHRLNPRAPLDIILAENMRSAAGFVRDGLRKHLPP
                     DFPVDTYTGLIETSIGKMVPIMPVSELEKDPLLIFAEPYNTLILDAAAFKNPVPGVAG
                     LAPVNNIRAWVDRKAFIHNLGHATAAYFGNYLFPEAVYMHEVLDHQEVRKFTREVMLQ
                     SADIIKATYPDEFTLQDLVDHTDDLILRFRNKALGDTIFRVGRDLPRKLAADDRFMGA
                     VHLAARFGMPCDKIIKAMSFGFFFSAKDEAGNSYQPDIDFRKAVQENPGTAMLNILGL
                     DLPGDSAVIKQLKEYLAAG"
     gene            complement(14000..15091)
                     /locus_tag="PLZ75_01930"
     CDS             complement(14000..15091)
                     /locus_tag="PLZ75_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOS71189.1"
                     /translation="MKTRTFTSLPHQGRRNFLKSNFFLGLAALSPGLILSGSCTSGRK
                     AEETEDLTENELLEKYLECLGGPFPEAPPLNPVLRETIRKDGYRIESVTYEGMPGERI
                     PAFILIPDGIDSSNPAPGIAVWHQHNGEYHLGKSEPAGLAGNPMHHTGVALVREGYVV
                     LCPDSLCFEERQDPTGILKGGNYERFEFLREVVRGRCLAWKDVFDMKQSVSMLCDRPE
                     VRKDYLGCYGHSMGSTHAWLVGPLEPRLKCVIGNCCLPTYEAIEEEHLLHCFPNFVPG
                     WKKYGDTPEIAALIAPRALHLNLGEEDDGSPIESARRGIERIAGVYEKAGVPENFTYF
                     IEAGAGHVLSEAMWDHVRKCFARHLGGNA"
     gene            complement(15242..17611)
                     /locus_tag="PLZ75_01935"
     CDS             complement(15242..17611)
                     /locus_tag="PLZ75_01935"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOS71190.1"
                     /translation="MYRKLFVFKGGMLFTLLSTLLIAFAGCRSTQTGPAAYVNPLIDT
                     KSPRWLFFSSACRPFGLVSLSPDTWVYGTWNSGYLYDTTTIRCFSHIHCWQLSGIPVM
                     PVTGEMKGHLGFEACKSRFSHDNEIVKPGYHKVILEDHNISVELTSTCRVGFHRYSFP
                     EGRAGHVLFDIGAELGHGKMDSASIRRIGDSELAGYTIMSPTHRRKKPCKVYFIAQFN
                     KPFSSFGGWKRPLSGPDAARELYHDTDDIHGIECGGYVTYNDPGGKALLMKVALSYVS
                     EDQARMNLNAELPHWDFDRIVKESYDEWNNELGKIEIEGGTHEQRVKFYTDLWHVLLG
                     RHIYSDYNGMYTDNTGPEPRIRQIPSDRNGNPVFNMHNSDSFWGTEFNLNILWSLVYP
                     QVMNNMAATLIEYYKNGGMIARGPSGGNYTFVMNGDQATPLIAAAYNKGIRNFDIDAA
                     FAGCLRNSEPGGIRSHSGYEVNPNKYMDYYVERGYVPEGIPGEGWHRTGCSLTLYFAY
                     QDWCMAQFAKGLGKTDIYEHYLKRSFNYRNVFDAETGWMRPREMDGSWYEDFSPVVEE
                     GSARGFTEGNSAIFTYYVPHNIPDLIRMIGGNKAFTDKLNNQFELAAPDRFITPHGLH
                     ARNWVDYENQPGFHMAHLFSHAGAPWLTQYWLRRLKDEIFSDITPYGGYNGDEDQGQI
                     GGFGVLTSIGLFDVTGGASTDPCYEITSPVFDKITIHLDRNYYPGKKFVIRTINNSPE
                     NIYIESARLNGKTLNSFRVPHRVITGGGSLEIVLSDKPSEWGIENVASE"
     gene            17933..18946
                     /locus_tag="PLZ75_01940"
     CDS             17933..18946
                     /locus_tag="PLZ75_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71191.1"
                     /translation="MKIENNQTVLEINLNGGSISDFHLKDLPVNPLSWRNKDTTMPPF
                     MGHFLCFDRWGPPSEGEKLNGFQHHGEVNTITWKVLSEPRINDGKISCSMTCTLPMGG
                     LELTRRIEMPVDGTVAFVREEIKNLNKYGRMFNIVQHVTLGPPFLDKTTLFDNNTEKG
                     FENKGDGSPDQEEPVLRWPRTVHNGKKVSLRHFHDPWPLVSSFVYSPGDKYGWTSASN
                     PGKNLLLGYIWKTEDYPWINFWRSMENDIPLAFGMEFGTTGLHEPFPVVAKKGKIFNR
                     NLYDFIDAGEVILKSYTVFLARIPDDYQGTDKIEITDSSVIVREKKRPSRDIVLTLKN
                     INP"
     gene            18943..20169
                     /locus_tag="PLZ75_01945"
     CDS             18943..20169
                     /locus_tag="PLZ75_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS71192.1"
                     /translation="MNSRERLISAINHKEPDRIPVDLGATPSSGISAVAWHNLVKYLG
                     LDLKNHVYDVIQEVTQPEMEILELFKVDILDIGRFFNNFPEYWHELELIEGIPGLYPR
                     WFNPVKQPDGSWLAAGPAGEYIAKMPADATFFDQTIFPYLDGYPDDYKNIAYDMSRVS
                     WGGFGFPPYDRMNEKDFWKMLRQTIIREREKTDRALLIGVGCNLFEWGTFLRRIDNFL
                     MDLYMDPVNVSRLLDKLLEMHMEFLSRICDAVGDIVEVIKFGDDLGSNTGPLMPPEIY
                     EKFFLPRHRMMCDYVKKHSSAHTMLHCCGGIYELIPGLIRAGFEILNPVQINAYMMEP
                     ERLKKEFGRDITFWGGGANSQNILNRASPQEVRDHVRHNVEIFSKGGGFVFNTVHNIL
                     PDVPPENIMAMFEAIR"
BASE COUNT         5655 a         4379 c         5058 g         5183 t
ORIGIN      
        1 atccctacca ggaagccaaa actgatacca caaaatcatg gtgcgtcgga ggtctctggg
       61 ccatgacgga atatcctgtc aacactcccg gtgcaaagga acttctgcag aaagcaaaag
      121 tgcagctcga tttcctgtac gggaaatacc ctataggcgc attcactgac ctgaacaatc
      181 cggccatgga taacaagggt ggtgcgatct tccaggtaca gtatctggtc gggacaagcg
      241 ggaacgggtg tattccctgt ttcgtacctc tttcaagcca taccacagtt ctcagcatcg
      301 agacggggac aataatcccc agccttgcat actttaattc atatgatccg gcagacaagc
      361 gtatcgtgga caggaattat ttttactaca gtgataccta cgcagccaaa tatgatgtaa
      421 atgaagggcc tgccggaaaa ttccccctgc ctttccttta taaatactat gactatgaat
      481 gcatgaaggt aaaaggtgaa tcaggcctga atttcaatct ttacagatat gctgacattc
      541 ttttaatgct cactgaagtg aactgggcgc tgacccagct tggtgtatcc gttccgtcag
      601 ccgatgttgt gaaggggatc aatgaagtca gggaaagggc aggactgtca acatttgcaa
      661 gtgcgggcga ccttaccctg aaggatataa tgagtgaacg tgcctacgaa ctgataatgg
      721 agaacaaaat gctgtgggat atgaggcgta cacgcaaagc ccttgttgac ggcgcaggtt
      781 catttatccg tattgaaaac ctggtcggac atcagccgac aagtttcaac tataagttct
      841 ctgccaaaca ccttctgtcg ccgatgtcag taaatgaaat cgccaataat gtgaaagttc
      901 tgcagaatta tggttggtta cctgttcaga ttggacaata atgaaaagat ctatgtaaat
      961 ttttaattgt atgaaaatga aaaaactcat attcttcacg ttaataatga tgctttttgt
     1021 cagttgtcag gacgaactgt acgatattcc aagggatgcc ggcgggaaag ccatattgac
     1081 agatatatcg agcacagcca caacaggtat cagtacactt gatgatcagt tttctgttac
     1141 tgcatatctt cccaatgcaa aatcaggaga catcatgaat gttgaatgtc tgcaacttca
     1201 ggtgccggca ggtgccacga ccactcaact gttaccactg ccgggaacac agaaaacagc
     1261 aacagtcggt tcggatctta aagcaaccat tacttttaca aggaccgaag ccaaacttaa
     1321 taaggccggt gattatgttg ttgtgacatt cggcggagaa accgaatccg ccaaacagag
     1381 ggttgacatg gttcctgcaa caacgacaac aaaacccagg gttgcaggaa aggatgttga
     1441 tgttgcacgc actgatgaaa cagcattttt taatgtaagt gttgttccca aaattggtac
     1501 ttttacagat actctcacag caaagaggaa gaacggtatt aacgatgttc tgactgatgt
     1561 gccgggatcc cccttctccg ggacccagcc atttctggtt ccgatttcgg gaaatgattt
     1621 tgctcctgac aaagatacca tgtactatgt ttttatggca acatccggat catatacgga
     1681 tattatagaa tattccgtgg tggtgcgcga tccttatttc tacctgaaaa aaaccggtgt
     1741 tacacttaat ctgggcggat catccgcagg caggaacctt ttaagaaatg ccgccgtggc
     1801 ggaggataat gccaatgcca caatagctgt ttcaggatca cttatactca agggaggatc
     1861 agcatggctg gcagccggca attcaataga attcgtaccc gccactgcag ctttgtacag
     1921 tacaaacagc accacggcat caatagctgc attcagtgcc ggaactccgt ctgaaacggc
     1981 ggatcccata gcaggtgagg gtatttttat ctttaaaata gtaaacgggc ctgatcctga
     2041 aaatgtcatt tacggcatga taaaagtaac atcggtaatc cccggtgttt cggttacctt
     2101 tgaatacagg ataggaaacc tgtatgctca tctggcggta atatcgtaaa cgatacttgc
     2161 aaagagggtg tttcagaaaa ggagcaccct ctttaaattc agataccgat gagttgcctc
     2221 ttatgcctga tcctccggaa taagggaagg gatgaggtaa tcagaaattg agtatctttc
     2281 tgatctctgt gttatcttat atttaaattt ttgattttaa gatcatgaaa aaaatccatg
     2341 tgcgttttat tatgatcctg gccggtttga tgatttccgt catcattgct gattgtcaga
     2401 ccatatcccc ttccggaatt gaatttaaga atgctccggt tctttttaag gaaggaggaa
     2461 aatcctttca gcagatattg gtgaattatc gggccgtcaa tccggaaaaa ctggtaataa
     2521 catcttcagg gaaggaattg ctgaatgcga accttacgaa aggggttaac agattccttt
     2581 taacccttcc tgcagtcagc agacccggga aaattacact gacagcccgt atcggcaacg
     2641 gaccggctga aaaatgccct tttacaatag ttccgccaaa aaaatggaag atctattttg
     2701 ttcagcattc acacaccgat ataggctata cccgtcctca atcggaaatt cttgcggaac
     2761 atatgcgcta tatagactat gcgcttgatt attgtgatca gaccgataat ctgcctgacg
     2821 atgcaaaatt caggtggaca tgtgaatcgg cctgggttac cagggagtat ttaagatcca
     2881 ggccatcgga acagatccgg aggctgaagg aaagagttac cgaaaaacgt gtagaagtta
     2941 caggaatgtt ttccaatatg gctgaaattg ccgatgagaa tgtaatgtac gatttccttc
     3001 agcctgtgaa ggaaatcagc agtcacggca taaatgttga aacagccatg cagaatgatg
     3061 tcaacggaat agcctggtgc atgcccgatt attttaaaaa taccggtgtc cggtatctga
     3121 taatgggcat aaacatgacc agatccatac tgcctttcga caaaccgact gctttctggt
     3181 gggaatcgcc atcaggagcc agactgcttg cattcagggc cgatcactat atgacgggaa
     3241 acaatttcgg gatcgagagc agggagctga aagcggagaa tatgctctgg catattgccg
     3301 atctggacga caggggttat ccctttgaca ggatagggat acagttttcc ggttaccgga
     3361 ccgataatgc ccctccttca acagcggcat gtgaactggt taaacaatgg aatgcgaaat
     3421 atgaatatcc caaaatcaga ctggcagtgg caggtgagtt catggaatat gttgacagga
     3481 attattccgg caagcttccg gtttaccgca atgcatggct tgactggtgg acggatggtt
     3541 acggatcaac ttcacgggaa acagctgaaa taaggaaaac acagaatata aagcaggttg
     3601 atgaaggact cctggcgatg gtgacaatta tgggcggtga aatgaacaat gcaattgaag
     3661 gaaaactcaa tcatataaat gagaatgcca tcttctttga tgaacataca tgcggtgcgg
     3721 atgaaagtat cagtcatccc tattccgaaa attccacaaa acagtggctt cagaaaggag
     3781 cttacgcctg ggaggcactg aaaagagtca ccctccttaa tgaggaagca ctggcaagat
     3841 tccagccatt tctttccaaa gcatcatttc cggttattta tgtgatcaat ccgatgggct
     3901 gggacagatc aggcaatata gaattatttg ttgacaatga ggttgttccg gttgagaaca
     3961 aagttaatat aatagatatt tcaacaggaa ggaaagtacc cgcacaggtg aaaacaagac
     4021 gtcgtgaagg ctcctggtgg atcattgagg taaataatgt tcccgcaatg gggtacaaag
     4081 ctctgaaagt tgagattata cctgagcccg caacaatcca ggctgaatca gaaaaaacag
     4141 aaattcttga aaaccaatac tacagactgg taatagataa gacaagcgga tcggtaagct
     4201 ctctctttga caaggcgctt gaccaggatc tggtggatcc ggaaaatcag tatcatcttg
     4261 gccagccggt acgggaaaca tccgaaagac gggatgtgcc tcctttcatc cgtacatcag
     4321 ttgccaatgt cagggtggat aagggtatta atggtgatgt atgggaaagc gtaagaataa
     4381 gctatgatct ggacggcttt gagaaaggca gcccgggcaa gccgggaggc atacaaacgg
     4441 aaataagatt atacaggaat gtccggaaaa ttgaattcag atacacggcc aacaaactta
     4501 tacttactga ccctgaagcc ctgtatgttg catttccctt tgcactgaaa gactccagaa
     4561 tcgtttttga aacaataggt ggtattctga cccagggaga acagcttccg ggatcttctt
     4621 ccgactggaa cgttgcccag aatttcgtgg ctgtacgcgg aaaaagcgga caggtgatta
     4681 ttgtcagcaa tgaggtgccc ttatggcatt tcagcgattt caacatgggt aagtttgaac
     4741 gctatccggt aaagggcaaa ccctggctct attcatgggt gatgaataat tactggttca
     4801 ccaatttcag ggcttatcag gaaggtgctt tcagctgggg ctatcagctg acatcgtccg
     4861 gggatacttc caatacattc gcaactcact gggcatgggg agaaagaaat ccttttctga
     4921 caagaacatt cccggccgga acaggcgaac tgaaaattcc ttcctttcag actctgaagt
     4981 tatcaggaac cggaaacgta ttgccggtaa acagccgtcc cgtctttaac cgcgaaagaa
     5041 ccatcctcct gcatttaaga gaacttgaag gcctgccggg cgaggtggaa atttcaagtg
     5101 ctgctcccgg acagatgata aggaaaatga ctgaagtcaa ttctttcggc aagccggctg
     5161 cccggctgtc atctgtaatc cctctgaaac cctatgaagt caaattcgtt gagctggaat
     5221 tctgaaaacc attaaatcct gtaagtaaat gtcgaaagta agattattcc ttgtttttct
     5281 gcttgtcccc atggcatgcg gcaacaatga tgtaacacgc tttgtggatc cgaacatagg
     5341 gggcgtggct ccccttctca caacaaaatg tcccaccgtc cacaggcctc acagcatggt
     5401 cagggttttt cccgttacaa aaccagggct gagtgaccgg tatctttcgg acaggatata
     5461 cggattcgga cttaacatgc cggcctacag gatgggccat gttaccagta ttatgccggt
     5521 accgggagaa ctctcatttg atcctgatgc aacagcatca tcctatgatc atgatcttga
     5581 agatgtgcac ccctggaagc atacagtttt tctggaagat catgatatag ttgcggaatg
     5641 gacaacaagc gaaagagctg ttgtataccg ttttgtgttc cggaaggatg ttccggcaaa
     5701 tgtgattttc agatccgccg gacaggcttc tttcaggatc agggggaata atacaatccg
     5761 gggatgggaa gaattcagga atacaaagca atacttccat gccgagtttt ctcttccctt
     5821 tgattcccgc ggaacattta acggatcgga gattattccc ggaagtgatg aggcagacgg
     5881 caggggaaca ggtgcctgta tatccttttt tgctccggga caaccgctgg aggtaaggat
     5941 aggtatatcc tacattgatg aggatcaggc aagagaaaat ctggagcgtg aaatgaaggg
     6001 taaaacgttt gagaaaatct gtgatgaaag ttacaggatc tggaaagatg cacttggtaa
     6061 aatatacgct gagggtggta ctgagagaca gatgaggatt ttctatacct cactgtacag
     6121 atgttttgag agaatggtga atatttccga atatggcagg tattacagcg gttacgacag
     6181 gaaggtgcac aatgatgaag gaaaaccttt ttatgttgac gactggttat gggatacatt
     6241 ccggagccag catcccctgc tctatattat agagcctgac aaacagcttg atatggcaca
     6301 gtcttatgtc agaatgtatg agcaaagcgg ctggatgccg acatttcccc agttttacgg
     6361 cgatttttcg gcgatgatag gatttcattc cactgcgctt gtatgggata tatatcagaa
     6421 ggggggaagg gattttgatg ttgaaaaagc ttatgaaggt tttatcaaga atgccatgaa
     6481 ggggaccctg gtgccatgga ggtccggtcc tatgtgcgga cttgacagtt tctatattca
     6541 gaacggctgg tttcccgccc ttccggaagg agctgaggaa acagagcccc tggttgacgg
     6601 ctttgaaaaa cggcaggctg tagctgtaac actggaacac agttatgatg actggtgcct
     6661 ggcgcagctg gcaaaagccc tggggaagaa tgaggattat gaatatttca gcaggagagc
     6721 tcagaattac aggaatgttt ataaccccga aaccggtttt atggctccga agatggccga
     6781 cggaaactgg gtggaaccct ttgatcccac cctgtctggc ggaatcggtt ccagaatgta
     6841 ttttgcggaa aataatgcct ggatctggaa tttcagcgtg atgcacgata ttccctccct
     6901 gattgaactg attgggggtg cggaaacatt tacggaccgg cttgacaggc ttttcaatga
     6961 accgacaggt attgccaaat ggcagttcat gggtcagttc ccggatgctt ccggattgaa
     7021 cggtatgttc cctgcaggca atgaaccttc attccatgtc ccctaccttt acaattacgc
     7081 cgggaaacca tggaaaaccc agagaaggat aaaggaagtg atggaactgt ggttcgatga
     7141 tgatccgctt ggcctccccg gtgatgaaga cggaggagcc ttatgcgcct ggtatgtatt
     7201 atcggcaatg ggattctacc cggtcactcc cgggaacgga ttgtatgcaa tcggaacacc
     7261 gttttttaaa aaagtcagca ttaatctgcc tgacgggaaa atatttacaa taaaagcccg
     7321 gaatatatca aaaaagaata aatatattca gtcggcaaaa ctcaacggaa aaccctttga
     7381 ccgggcatgg atcagacatt ccgagatcac cgggggaggc acacttgagt ttgtgatggg
     7441 tgatcgcccc aacagggaat ggggaaatga tccggcagtg cttgagcagc tgacagctga
     7501 taactgaatt cataaataat atatttccag gcaatgacca gatttatctt agccctgctg
     7561 tttatatcgt tcagcctttt atcatgcagc agggatacaa aaattgtatg gagcataggc
     7621 agtgaggacg gatcttcggc agatcttgca ctgggtccgg acggattcag ggatttcctg
     7681 agccgggatt ttggttttga ggataagtat tttcttattg gccattcggt ggacagggag
     7741 tcttttccct atgttcttcc gggcccggcc gatacctggg gaggaacatg gtccaccgcg
     7801 ggctggagga cacatcagat aaatattttt ttcgggatcc gggaaatgca gggaaagagc
     7861 atgcatggca agtggaaact ggttatcagc cttcttgatt tttcagggag attcccgcca
     7921 ttgctgaagg caagcataaa tgaatatgac cggaaaatca ggctgtttgc cggtgagtat
     7981 cagccggctg atcaggttca tcccggaatg accgcggctg tcaccgatac ggcatccatt
     8041 tcaggcgatc tgaaggatat tcacccccgg aagattgaaa tacctgttga tccgggaatc
     8101 atcaaacgcg gaggcaactg cattacactg acaattctgg aaggctcatg gattatgttc
     8161 gatgagcttc accttgaggc tccgggaaat gtatccctta ttgtgccgga agaggttttt
     8221 atccgggaag tgaaagcttc ctcatatgaa cttgatcagg gggaagacag gattcagccg
     8281 ctcctggtga ccgcggtaca tcttgacgga tcaccggaaa taagtgtgga actggacggg
     8341 aaggagattt tcagggaaag aactgaggca ggtatctgtc agtttgaggt gccgatgccg
     8401 gcaactgaca aaagaatcaa atcgggatat aaaatatatt cagggaatgt tctgcttgag
     8461 gaaggaactg ttatacgttc acctcagaaa gccggaacgc ttgcagatta tgttgacaca
     8521 agaaccggaa cggcacattc aagatggatg attgcacccg gtccctggat gcctttcagc
     8581 atggtaaaac tcagtccgga taatcagaat ccgggatggc aggccggcta tgaaccttcc
     8641 tttgagagta taggtacttt cagccatatt catgaatgga ctctcggcgg acttggtatt
     8701 ttcgcttcaa atggcaggct tatcacaaag ataggggatg aacttctgga tgaggaagga
     8761 taccgttccg gaatcaataa aaggaccgag gaagcccctt tgggttatta cagggccgat
     8821 ctcaccaggt acggcataaa ggcagagctt acagctacca cccgctgcgg tttcctgaga
     8881 ttcacctttc cttcgggcag ggacagctca agaatacttg ttgatttaaa agtgccggct
     8941 gaatacagct atgaactgaa agagataaat ataaaggcga caggaaaaaa ccggattgag
     9001 ggtttttgcc gccagtttgc cccgagggtc tggagcaatg atgcacagca ggactatact
     9061 gtgcatttcg tgcttgaaac ggacaggcct ttcaaaagta tgggaagctg gctgaacggg
     9121 aaaataattt attccgattc cctttatgcg aaagaggcag aggatgcagg gatattcctg
     9181 gaatttgata cccggaagca gcgtgcggta cagcttagat cgggtatttc ctatgtcagc
     9241 atcccgaatg catcggagaa tcttgaaaca gagatctccg ggcctttcgg ctgggatttt
     9301 gatgccgtca ggaacaataa tgtaaataca tggaatgata tttttgggcg ggttctgata
     9361 aaaaccagcg acaggctgga gaaagtacgc ttctataata acttttacag ggctttgtgc
     9421 agcaggaaca ttttttccga tgtaaacgga gaatggatct ccaccgacgg tttgacaagg
     9481 aaatttgaaa atccggatca tgttgccctg ggatgtgatg cattctggaa caccttctgg
     9541 aacctgaacc agttctggaa cctggtaacc ccggagtggt ccgaaagatg ggttaactca
     9601 cagcttgcaa tgtatgatgc caacggatgg ctggcaaagg gacccgcggg gatgaactac
     9661 atcccggtaa tggtggctga acatgaaata ccgcttattg taagtgcata ccagatggga
     9721 gccggcaatt tcgatgcaga caaggcactg gaggctgctg ttaagatgca gaccacccct
     9781 gcacagaaag tatttaatgg ttttgccggt aacagggatc tgattccctg gctccgttat
     9841 aaatatgttc cgtatgacaa gggaagattt tccaacagca tggaatattc atttgatgac
     9901 tggaccgttg gtcagctggc cagggcactg ggaaatgagg agctataccg gaaattcaat
     9961 gaacggggct actggtggcg caatgcgata aatccggaaa atggctattg tcacatgaga
    10021 cacagtgatg gcacatggct gaaggatttc gatcccttcc tctcaggtgc caacaggcac
    10081 tatgttgagg ccaatgcatg gcagatgacg ttttttgtac cgcaggatgt gcccgccctt
    10141 gttgacgcca taggaaagga ccgcttcaat gaaaggctgc aatggggctt tgaacagagt
    10201 gagaaatgga gatacaacgc tccgaatgac cagtactgga attatcctgt tgtacaggga
    10261 aaccagcagt caatgcattt ttcctttctg ttcaactggt cgggaaaacc atggctgacc
    10321 cagaaatgga ccagatccat tatagacagg tattacggaa cgggaacagc caatgcctat
    10381 ctcggggatg aggaccaggg ccagatgagt gcctggttcc tgatggcatc aatggggctg
    10441 ttccagacag acggcggatg cagggtcagt cccgtttatg aactggccag tcccgttttt
    10501 aaaaggattg aaatcgacct gggaaacaga tacggcaggg gagaaaaatt cataatagaa
    10561 gcagaaaaca gttcaaggaa aaacatgtat atcaggaagg caatcctcaa tggcactgag
    10621 cttgaatcat tccttattcc tgcagctgaa ttgctgaagg gaggttcact gatgcttgaa
    10681 atgggatccg aaccggatac aacgcttttc cggtagcagt tcatcagaat gttttatatt
    10741 tttgaaagac atattttata aaagaacccg gtcagaacgg ttcaggaatt tgtaataata
    10801 atttacagat ctgacaataa atgttttaca aaccctgccg gtaaagggtt aaagaatttt
    10861 aagcgattgc atatatcagg cgcaaaacaa ttaattaaag taaaaggcta tgagaagaag
    10921 tgaagtaaac aaaatcatta tggattttga aaggtttctt gccgaacata agttttacct
    10981 gcccgtgtgg gcatactgga aaccggagga atggaagggg aaatatgata tctgctccga
    11041 gatcgttgat aataagcttg gctgggatat caccgatttt ggcagcggtg atttcaaaaa
    11101 gactggactg tcacttttta cgatacgcaa cggcaactgg gacaagaaag acaagatgta
    11161 ttgtgaaaag ataatgatgg ctgatgagga acaggaaact cccatgcatt atcactgggc
    11221 caagacggaa gatataatca accgtggcgg cggaaatctg gtgatggagc tttattatgc
    11281 aacagatgat gacagattgt ctgaagatcc ggttacagta agcattgacg gtgtgctgac
    11341 aaccgtagag gcaggcagac ccctcatact taaacccggt cagagcatct cgctcaagtc
    11401 aaaactctac cacaggtttt acggggaaaa gggcaggggc aaagtcctca tcggagaagt
    11461 cagccttgta aatgatgatg ccaaagacaa taagttccat gaggagatag gaaggtttcc
    11521 ggagattgat gaagacgtca gtccgcaccg cctcctggtg aacgattatg aaaaatacct
    11581 ctgacagcag acaaaaaaac aatataagtc agatactgtt gtgaagagaa aaattatcgt
    11641 atccggtaca ggctgctgcc tggtggatct gcttttcaat aatattgatt tcagcagtga
    11701 tcagatcctg gacttccttt cgaaggagag gggtgatggc ggactgtcgc cggggaagct
    11761 ggtattccag gaagaattcg agaagttcag cggaaaaccc cttgtttcgg tactggatac
    11821 cctcaccggc gggagaaaac cggataagat caatatcggg gggccttcaa ttgtatcgct
    11881 tatacatctt gcccaggtga caggacgtga aaaatgtgag gtcaggttcc atggcagccg
    11941 ggggaaggat gatattggtg aatatctgct gacagccctg aaaaggaccc ctgtggttct
    12001 gaaggattac aggattacgg aaaggcggac gccgtcgact gtagtgcttt cagatccgca
    12061 gtacgacagg gggcatggtg agaggatgtt tattaattca ataggggcag catgggatta
    12121 cggaccggat gatctggaca gtgaattttt cggttccgat attgttgttt tcggcggtac
    12181 agctcttatg ccgaaaatac acgacaggct ggccggtctt ctgaaaaaag caaaatcagc
    12241 aggagctgtt acggttgtga acactgtatt tgatttcagg aacgagaaaa gagacccctc
    12301 aggcaggtgg ccgctgggtg agagtgatga aagctacagc catacagatg ttctgatcac
    12361 ggatatggaa gaagctctgc gtctcagcgg aaagcacgat atagaatctg cagcggagtt
    12421 ttttataaag aaaaaggttt cgtcatttat tataaccaac gggtcgggaa atattaccct
    12481 ttattctgac ggtcgcttct tcaggccatc tgaaatggca gagatgcctg tatcacaaag
    12541 aattatcttg gagataaaag atgttcggga aggcgacaca accggatgcg gggataattt
    12601 tgccggagga ataattgcat cggtcgttga acagatgcat gacggcatac ggcatcccga
    12661 cctcagggaa gcatgttcct ggggaattgt ttcgggagga tatacctgct tttatatggg
    12721 gggaacctgg tttgaagaaa gggcaggaga aaaacttgaa agaatcagac cctactatga
    12781 atcctacagg gaacagaccg ggcattgatg atcacagtat cgtcatattc ggtgccggca
    12841 ggataggacg ttccttcatc ggtcagctgt tcggcaggag cggatacaga ctggttttca
    12901 tagatgcaga tcctgtgctt gtcggaagac tgaatgcggc aggatgctac agggttataa
    12961 tgaagggaga gaaggaagaa gagatagttg tatccaacct ggaggcaata catgctgctg
    13021 acactgttca tgctgccggt gcagtatctt cagcaggaat tcttgcagtg tcggtcggca
    13081 tgaatgcaat tgaaaaggtg gttccgtctg ttgccgcagg aattgatctc cgtcaccgtt
    13141 taaacccccg ggcacctctt gatatcattc tggcagagaa catgagatct gctgccggct
    13201 ttgtcaggga tgggctcagg aagcatctgc ctccggattt tcctgtggat acctatacag
    13261 gcctgatcga aacgagtatt ggaaagatgg tgccgatcat gccggtttcg gaactggaga
    13321 aagatcccct gctcatattt gccgaaccgt acaatacact gatactcgat gcagcagcct
    13381 ttaaaaatcc tgttcccggg gtggccggac tggccccggt gaacaatatc agggcctggg
    13441 ttgaccgcaa ggcatttatt cacaatctcg gacatgccac tgctgcatat tttggaaatt
    13501 atttattccc tgaagcggta tacatgcatg aagtccttga tcatcaggaa gtgagaaaat
    13561 tcacgaggga agtgatgctt cagtcggcag atattatcaa agccacatac ccggatgaat
    13621 tcacccttca ggatcttgtg gaccatacgg acgatctgat acttcgcttc aggaacaagg
    13681 ctttgggaga cacaattttc agggtaggca gggatctccc ccggaaactt gctgcagatg
    13741 accgttttat gggagcagtt catcttgctg cccgctttgg catgccctgc gacaaaatta
    13801 taaaggcaat gtcgttcgga tttttcttca gcgcaaagga tgaagcagga aacagctatc
    13861 agcctgacat tgatttccgg aaggctgttc aggaaaatcc cggaacagca atgctaaaca
    13921 tcctggggct cgatctgccc ggagacagtg cagttataaa gcagcttaag gagtaccttg
    13981 ccgcagggta aaaccattat caggcattcc cgcccagatg cctggcaaaa catttcctta
    14041 cgtgatccca cattgcctct gagaggacat gtccggctcc tgcttcaata aaatatgtga
    14101 aattttcagg cacgcctgcc ttttcatata ctccggcaat ccgttctatc ccccttcttg
    14161 cagattcaat gggacttccg tcatcttctt cgccgagatt gagatgcagg gccctgggtg
    14221 caatcagtgc cgctatttca ggagtatcgc cgtatttttt ccatcccggg acgaaattcg
    14281 gaaaacagtg gaggaggtgt tcctcttcta tggcttcata ggtaggcagg cagcaattgc
    14341 cgatcacaca cttcagcctt ggttccaggg gaccgacaag ccaggcatgt gttgatccca
    14401 tagagtgccc gtagcagcct aaataatctt ttctgacctc aggtctatca cacagcatcg
    14461 agactgattg cttcatgtcg aagacatcct tccaggccag gcatcttccc cttactactt
    14521 cacgcaggaa ctcaaagcgt tcatagtttc ctcccttaag tattccggtc ggatcctggc
    14581 gttcttcaaa acacagggaa tccgggcaga gcacaacata accctcgcgt acaagggcaa
    14641 ctccggtgtg atgcatcggg ttgcccgcca gacccgcagg ttcagattta cccaggtgat
    14701 attctccgtt gtgctgatgc cagactgcga ttcccggggc cggattagag gaatcaatgc
    14761 cgtcaggaat gagaatgaat gccggaatcc tttctcctgg cattccttca taggtgaccg
    14821 actcaatacg gtaaccatcc ttccggattg tttcccgcaa tacgggattc aggggcggtg
    14881 cttccgggaa cggtcctccc aggcattcca gatatttttc aagcagttca ttttctgtca
    14941 gatcttcagt ttcctcagct tttcgcccgg atgtacagga accggaaaga atcagtcccg
    15001 ggctcagggc agccagtcca aggaaaaagt ttgatttcag gaagttccgc ctgccctgat
    15061 gcggtaaaga tgtgaatgtt ctggttttca ttgatactct tattttattg ttggcatact
    15121 cctgcaaaaa tcgtcacggg tgttgtataa gtgaccccgg gtactaatga aatattttgc
    15181 cgggattccg tatctgagga tttgttcctg ctgattgaaa gcaagggttc tcagctacgg
    15241 ctcattctga agctacgttt tcaatacccc attccgatgg tttgtctgac aatactattt
    15301 caagggatcc tcccccggtg atcaccctgt gaggaacgcg gaaactgttc agtgttttcc
    15361 cgttcagcct ggccgactca atgtatatgt tctccggact gttgttgatt gtcctgatca
    15421 caaatttctt ccccgggtaa taatttctgt cgaggtgaat tgtgatttta tcaaatacgg
    15481 gactggttat ctcgtagcag ggatcggttg aagcaccgcc tgttacgtca aaaagaccga
    15541 ttgaggtcag taccccgaaa cctccgatct gcccctgatc ctcatccccg ttatagcctc
    15601 cgtagggagt aatgtcgctg aagatttcat ccttcagcct tctgagccag tactgtgtaa
    15661 gccagggggc accggcatgg ctgaaaagat gtgccatatg aaatccgggc tggttctcat
    15721 agtcaaccca gttccgggca tgcagtccgt gaggtgtgat aaacctgtcg ggcgctgcca
    15781 gttcaaactg attgttcagt ttgtcggtaa aggctttgtt gcctccgatc atcctgatca
    15841 gatccggaat attatgcggc acgtaatagg tgaatatggc agagtttcct tcggtaaagc
    15901 ctcttgcaga accttcctcc acaacgggac tgaaatcctc ataccatgat ccgtccatct
    15961 ccctcggccg catccatcct gtttccgcgt caaaaacatt ccggtagttg aaagaacgct
    16021 tcaggtaatg ctcatagata tctgtttttc ccagcccctt tgcaaactgg gccatgcacc
    16081 agtcctggta ggcaaaataa agggtgaggg aacatccggt cctgtgccat ccttcacccg
    16141 gaataccttc aggcacatag cccctctcga cgtaataatc catgtatttg ttcggattta
    16201 cctcatagcc gctgtgtgag cgtatcccgc cgggctccga attcctgagg cagccggcaa
    16261 aagccgcgtc aatatcaaaa ttgcggatac ccttgttgta tgctgcagca ataaggggag
    16321 ttgcctgatc gccgttcatt acaaaggtat aatttcctcc gcttggcccc cgtgcaatca
    16381 tgcctccgtt cttgtaatac tcaatcaggg tcgctgccat gttgttcatc acctgcggat
    16441 aaaccagcga ccagaggatg ttcagattaa attcggttcc ccagaagctg tcggaattat
    16501 gcatgttgaa aaccggattg ccgttcctgt cggaaggaat ctgccgaatg cgcggttcag
    16561 gacctgtatt gtcagtatac attccgttgt aatcactgta aatatgacgt ccaagcagaa
    16621 catgccacag gtctgtatag aatttaaccc gctgttcatg agttcctccc tctatttcaa
    16681 ttttaccaag ctcgttattc cattcatcat acgattcttt tactatcctg tcaaagtccc
    16741 aatgaggcag ttcagcattg agattcatgc gggcctgatc ttcacttaca tacgacaggg
    16801 caactttcat cagtaaagct ttccctcccg gatcattata ggtgacatag cctccgcatt
    16861 caattccatg tatatcatct gtgtcatgat acaattccct tgcagcatca gggccgctga
    16921 gcggcctttt ccatcctccg aatgatgaaa acggcttgtt gaactgtgca atgaaatata
    16981 ctttgcaggg ttttttcctc ctgtgggttg gcgacatgat agtataaccg gcaagttcac
    17041 tgtcaccaat cctgcggatt gaggcggaat ccattttccc gtgtcccagt tcggccccga
    17101 tatcaaataa tacatgccct gccctgcctt ccgggaaaga gtatctgtga aacccgaccc
    17161 tgcacgtgga agtgagttca acgcttatat tgtgatcctc aagtattacc ttgtgatatc
    17221 cgggttttac aatttcattg tcgtgtgaaa atcgtgattt gcaggcctca aagcccaggt
    17281 gacccttcat ttcgcctgtt accggcatga ccgggatgcc ggacaactgc cagcagtgta
    17341 tgtggctgaa acaacggatg gtggtcgtat catacagata gcccgaattc catgtaccgt
    17401 acacccatgt gtcgggactg aggcttacga gtccgaaggg cctgcatgcc gatgaaaaaa
    17461 acaaccatct cggacttttt gtatcaatca ggggattgac ataagcggca ggaccggtct
    17521 gggtgctgcg gcatccggca aaggctatca gaagggttga caataaggtg aatagcatgc
    17581 cgcccttaaa gacaaatagt tttctgtaca tggctgtaaa ataatagaaa caacaatgag
    17641 atatacccgg aatatacaca aatataatca gtctgaccgt tggatacttt ccggaaaaca
    17701 tttattttta ttaaacacag ggatttatcc cggcctgaga gaataataat agtatatttt
    17761 atataacgct ttgataacct atatgttatt tatgaaaaga aaaatgatga cgatactgtt
    17821 tttatttctt ttattactct tttcgggtca gataaaaaac agcgggatat cttctgattc
    17881 ccaggcaaaa aaatatgccg gcgttcaaaa tacaactttt gcaaacactg ttatgaagat
    17941 tgaaaacaac cagactgttc tcgagattaa tctgaatggc gggtcaatat ctgattttca
    18001 cctgaaagat ttgcctgtga atcctctttc ctggcggaat aaagatacca ccatgcctcc
    18061 gtttatggga cattttcttt gttttgacag atggggcccc ccttctgaag gtgaaaaatt
    18121 aaatggattt cagcatcacg gagaagtaaa caccattacc tggaaggttc tttccgaacc
    18181 ccgtatcaat gacggaaaga taagctgttc catgacgtgt accctgccca tgggcggact
    18241 ggagctgacg aggaggattg aaatgcctgt ggacgggacc gtggcttttg taagggaaga
    18301 aataaaaaac ctgaataaat acggccgcat gttcaatatt gtccagcatg taaccctggg
    18361 acctcctttt cttgacaaaa caacactttt tgacaataat accgagaagg gttttgaaaa
    18421 taaaggagac ggaagcccgg atcaggaaga acccgtatta agatggcccc ggacagttca
    18481 taatggcaaa aaggtaagcc tgcggcattt tcacgacccg tggccgcttg tttcttcctt
    18541 tgtttacagc cccggggaca aatatggatg gacatcagcc agtaatccgg gtaaaaatct
    18601 tcttcttgga tatatctgga aaacagaaga ttatccctgg atcaatttct ggcgctctat
    18661 ggaaaatgac atcccgcttg ctttcggaat ggaattcgga actaccggat tacacgaacc
    18721 tttccctgtt gtggcaaaaa agggaaaaat attcaatcgt aatctttatg acttcatcga
    18781 tgccggtgag gtgatcctga aatcctatac ggtgttcctt gcgcggatcc cggacgatta
    18841 ccagggaacg gataaaattg agattacaga ttcttcagtg attgtcaggg aaaagaaaag
    18901 gccttcacgg gatattgtac tgactcttaa aaacataaat ccatgaattc aagggagaga
    18961 cttatttcag caataaatca taaggaaccc gacaggatac ccgtcgatct gggtgcaacg
    19021 cccagttccg gaatctcggc agtcgcctgg cacaatctgg taaaatattt gggacttgac
    19081 ctgaaaaacc atgtatatga tgtgatccag gaagtaaccc agcctgaaat ggaaattctg
    19141 gaactgttta aagtcgatat cctggatata ggaagattct ttaacaactt tccggagtac
    19201 tggcatgaac ttgaactgat agagggtatc cccggccttt atccccgctg gtttaatccc
    19261 gtgaaacaac ccgatggcag ctggcttgct gccgggcctg caggcgaata tattgcaaag
    19321 atgccagcag atgccacatt ttttgatcag accatatttc cctatctgga tggttatcct
    19381 gatgactata agaatattgc ttacgacatg tccagagtgt cgtggggcgg attcggattt
    19441 cctccctatg acaggatgaa tgaaaaagat ttctggaaaa tgctcaggca gacaatcatc
    19501 agggaaaggg aaaaaaccga cagggcctta ctgataggtg tgggatgcaa cttgtttgaa
    19561 tggggtactt tcctgcgcag gatagataat ttccttatgg atctgtacat ggatcctgta
    19621 aacgtaagca ggttgcttga caaacttctg gaaatgcata tggaatttct ttcgaggatc
    19681 tgtgacgcgg tgggggatat tgttgaggtt ataaaattcg gggatgatct gggctcgaac
    19741 acaggaccgc ttatgcctcc ggagatttat gagaagttct tcctgccacg gcacaggatg
    19801 atgtgtgatt atgtaaaaaa gcactcttca gcccatacca tgcttcattg ctgcggaggg
    19861 atatatgaac ttatacccgg actgatccgc gcaggttttg aaattctcaa tcctgtacag
    19921 ataaatgctt acatgatgga acctgaaagg ctgaaaaaag agtttggcag ggatattaca
    19981 ttctggggcg ggggtgccaa ctcacaaaat atacttaaca gagcttcacc gcaggaggta
    20041 agggaccatg tacggcataa cgtggagata ttttcaaaag gcggaggatt tgttttcaat
    20101 actgtccata acattctgcc cgatgtgcct ccggagaata tcatggcaat gtttgaggcg
    20161 atccgttgaa ttcatttact gccttgtagg ctgcaactat attctgcggg ggaacctctg
    20221 acaatatatt atgaatgggg gcaaagacaa agccgccgtc acgggaaaat atttc
//
LOCUS       DAOSPX010000019        20237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2825_length_20237_cov_4.523288, whole genome shotgun sequence.
ACCESSION   DAOSPX010000019 DAOSPX010000000
VERSION     DAOSPX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2825_length_20237_cov_4.523288"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1844)
                     /locus_tag="PLZ75_01950"
     CDS             complement(<1..1844)
                     /locus_tag="PLZ75_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71193.1"
                     /translation="MKTIYAISAALCIIICTAACSNNKHPRPVQHHRDLPFVQDYSIK
                     YPADDEKTVFHNVVSDRNGYIQVFSSAGLLKPSGGRFLFPGKLVKDHHYRPLSDKKIS
                     GLGTCLGQMLYIDDTALLSNAWAGKLFVKHNMPGARILESGNDFAFLVSDGRKLAFLN
                     DREILWEDVYHDEISDIKFENRNNLFWILGKKGIGIFDPELKKTEKILDRENITCLEA
                     SGGKIIAGTGEGYFLIDAADRKADGELHNRLPCTDITVIKEIDGSLWFGSSRGAFRLR
                     EDGKYDYYASERWLPSDNVRDISEGPDNSVLILTDRGLGRIFFKKMTLEQKAAFYEKQ
                     VRERHIRHGFNATLSGMINGDLSSGSLSDSDNDGLWTSMYLAGQVFRYAVSGSGEALD
                     NIRESLDAMERLYTVNPVPGFPARSFERRGYKYEDIPWRRAEDPEWDWKSTTSSDEAI
                     GHIFAFGTIAELVSQPDIRQKAIMLIDTLMSHIVRNNFYLTDWNGEPTLWGRWNPEYV
                     NARPVMVGDRKLNSSNIIGMLQTAYHFTGREKYRDTAFYLMREHGYLENLMRPFREIG
                     PAPETADPWSRMLSESWNHSDDEMYYCGYWGLYRYAFNDTLKAKYRQA"
     gene            complement(1858..3156)
                     /locus_tag="PLZ75_01955"
     CDS             complement(1858..3156)
                     /locus_tag="PLZ75_01955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS71194.1"
                     /translation="MNYNFSDKPAVLGGTPVRDTRTMPWPEWPVWDENEEKALIKTLR
                     SGIWSYNGPLENQFNKMFADYNGTEYALSAANGTVTLQLALEACGIGLGDEVILPGLT
                     WQATAATVLDVNALPVLADVCEDTWCIDPEKIREAISPRTKAIIPVHLYGSFADMDEI
                     MKIAGEHGLFVIEDCAHKHGGEWKGKKAGSIGDIGSFSFQLSKLLTAGEGGALTTSSF
                     SLYEKLDALRNCGRRPEPAGFDKTDKGAGFYFDAGNFLQSGNYRITEFQAAILIEGLR
                     RLPEQNKRREENALYLNSLLEKLPGIKPVKRDERQTAGAYYNFAFRYNSKAFSDLPAE
                     KFRQALSAELGIEIAASYIPLNKCSLYAPCTKAPRHRLSEEYWKAIDPSRFDLPVCDR
                     IHSGESVCVHHKVLMGTRKDMEMFADAVRKIYENAVKLLA"
     gene            3479..4408
                     /locus_tag="PLZ75_01960"
     CDS             3479..4408
                     /locus_tag="PLZ75_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOS71195.1"
                     /translation="MKKFCSVVLIAFATGPVLLVSCKKEEPKGPPVAGIFYSVVEKKV
                     AFTALTKRADTWLWDFGDGQTSTEMNPVHIYQEGGIYDVKLTVTGSGQSAGASTVVSL
                     ALSNIQMLTGGLKYPDGKKWKISSAHSDKDAFTLADDKFTVIQPVATGMLGSVGLGLG
                     EVYDDIFIFKHDGSYIHSPQHGGAFAGLLYSMLTKQNILNVTPVSQSFGLCYTAWTPQ
                     TATFTLNEKKDLEITTVSPDGNTPADRVYKDVMALSFSGTEFIGFMDFTRECIIQSLS
                     PSKMRLAMFVCTTQKAHFNKPSHVVILTFETVP"
     gene            4454..6070
                     /locus_tag="PLZ75_01965"
     CDS             4454..6070
                     /locus_tag="PLZ75_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOS71196.1"
                     /translation="MKHHIKINDIVRYVIPAMLLSFLLTHNLMADRIPDYGINPGSSI
                     AGQQGIVITGRVTAETDNALMPGVSIVVRGTPRGTVTNSAGEYSIEVPGSDAVLVFSF
                     IGYTTEEVTVGSQRTINVVLRESVEALEEVVVTALGITRREKSLGYSVGRVSGEDLAR
                     VVQENAISSLAGKMTGVQISSTGGTGSSVSMIIRGATSLSNDNQPLFVIDGVPVVNTL
                     NNIAGFGSDNRVDYGNAISDLNSDDIESVSVLKGPSAAALYGSRAGNGVVIITTKSGM
                     KSKGMRVTISTNNVFDKPYKYFPVQKKFASGYFSFTPEDLPPGYVMKVNPAEAAGAGI
                     ELDKEYFAVKWDSPRDANGVQIPVELVSHPDNLPNFVQTGITSTNDISVTNNTDMMNY
                     RIGVSNMSNRGIVPNSDLFRNNLTAATSLRVRENITLSSNININRSWSNNRPAGNRGA
                     NPLEWAYKIPQDTDIRDLRNYWEEGQEGIQQRTPYNGIYNNPYFLAYEINNSFLRDRI
                     FGNLKAEWKISRDFTLMGRYSLDQFSEKRE"
     gene            6074..7900
                     /locus_tag="PLZ75_01970"
     CDS             6074..7900
                     /locus_tag="PLZ75_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS71197.1"
                     /translation="MAPSYTKESNNGAYGVQDLTSYERNADVLATYSKTTDNFSLSAS
                     AGGNALYKKGTSVSSSSKSGAGLTVPNVFTLSNIKSGSLDYSSYWSQKAIYSIYGLIN
                     FGFRDMIFLDITGRNDWSSTLPRENWSYFYPSASLSIIVNEMLNMGGGVELFKIRGGW
                     AKVGNDTDPYQLFSTYGNAGQWGDATRLTKSGQILNPNLKPEEAVSREAGLDLNLFRN
                     RIRFEGTYYVVENRNQIIRNIPVASSTGSDNVNINAGLIRSKGWEFMLGGTLLKTPGW
                     YWDVSANFSRNRTRVIEIAEGIDVIKFWEDARGGAWSYVGDEIGAIYDAEVITVTDKN
                     SPYYGYPILDEGDLEWGAVEMRYTKNKIGNYNPRFIMGFRSSLSYKAFSLNMTVDWRN
                     GGQFISQTQRYMAEDGSSQIWLDNLIHPGGRTGRELRDWLVQNEDKYIRNGFHVVGGP
                     TKEYGGFRENFSGVYVNDGIFVPGVWAKDDGEGGVTYIENLGEDGTVIVPYVVSYPWG
                     FAKPSMFDSDCVKLREISLSYSVPRDVVNRLRFIHDIALSLYSRNIILWTKAKSGIDP
                     ERAFQAESSTGGTRGTQFKQGIERFNLEPWVIPVGIKLDITF"
     gene            7985..9637
                     /locus_tag="PLZ75_01975"
     CDS             7985..9637
                     /locus_tag="PLZ75_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOS71198.1"
                     /translation="MKAIKHKISALLTVLLLMFISCQNLEELNINPNGVDPANARPFL
                     LMATVLTQTGQNVVGLGFGDLAGVMQHTQKDGWAGGHNSYDWSDQNWSSFYGILRNAE
                     EILKKSESDGLEFYKGVALIIKAYNYGMITDLWGDAPFSEALKGEAGNLKPSYDSQKD
                     IYTGILAWLETANTLLSKDQNSYPDINTTQDVMYGGSVSNWRKFANSLALRYYMRISS
                     KEPSVARTGIEKIAADPEKYPLILDARDDANIAYVGNSTSDSWPCNTTFDVSESNWRR
                     LKMCSTLVEKLQELNDPRLALWASKIAVPLVLDPDRPDDFDEIIDGRRVIAQNIADTY
                     EATYDVLLNLDPEYVGLPPAWSILPQAYNLCPNLEQAPLNPHASHLSNMYKQAAGPHL
                     KARLLSAAEVNFILAEAALNGWAAGGSAKDYYEAGIRASLDAWELTGSYQAYITEDGV
                     AWDGTQGQLMGQKWIAGWTAAAESWFDYRRTGYPELLPGKIVKRSAIPLRFYYGTNEL
                     LYNAEKAEAAIERLETTSFSSTDGKNSPWSKSWLLQGTGKPW"
     gene            complement(9744..10760)
                     /locus_tag="PLZ75_01980"
     CDS             complement(9744..10760)
                     /locus_tag="PLZ75_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOS71199.1"
                     /translation="MAIRSKYSLLKRIVRSGLLALAAVIVVFVLWMSLPRLWSSLSPG
                     KPPAGYFFTPATYAAVWLGVEKLMDLGPEIPENIEEFRDIEYKNINGRSLQLDIYRDK
                     NSPGPAPLIAFIHGGSWRSGKRSDYLPYLIGLARKGYVTATVSYRLLRDAPYPACVED
                     IADAVIWFHSNGDQYGYDTSRMALAGGSAGAHLALLAAYGWKPAVLHDTIPGQKTRGK
                     IKAVADIYGPVDLTTEYARNHRLVTSFLNSSWDENPDIFREASPVSYIDGNDPPTLIF
                     HGTSDRLVPVSQSDRFRELLDSAGVPCVYHRIPGWPHTMDIVQRVNIYCQEKMNDFFL
                     QYLK"
     gene            complement(10764..12419)
                     /locus_tag="PLZ75_01985"
     CDS             complement(10764..12419)
                     /locus_tag="PLZ75_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71200.1"
                     /translation="MKRLKIIFKAAGIALLAVILAIIVLSFKNMKDHNPGYRADIEIH
                     GGKVSALKAGFSAVSITPEVPDRWTDADNNAKYEPRRGDTFTDGNGNGVFDPVWIAGF
                     GNKRAANGVHDDLWARTMILDDGKSRLAIVVLDLIGFMNNDVIDVRSRIPEDAGITYT
                     IIISTHTHEGPDMLGLWGKSPLKSGVDGKYKEYVKRRIVESVIAATESLRPAYLEVSE
                     DLTGAEHLVTDTRKPDVYDSGLRCIKVLDAENGSTLGSVISWADHPETLWGNNLLITS
                     DFPHFVREGVEKGVFRNGSTDGERTEAGFSRGSSLAGIGTEETVSGGGNQGMEGTGGT
                     AIYVNGAIGGLMTTHPSLSVKDPFSDTEYSEPSFAKAEAQGKQVALLALKAMQNPAAR
                     IDSATLSLVARTVILPVRNKLFRLGTMLGILDRGMTGWMKMRTELAVFKIGPLSFATM
                     PGEVYPEIVNGGIEAPEGNDFSTGPAELPPVREMMPGRFKFFFGLANDEIGYVIPKSQ
                     WDVKSPYTYGRNSPPYGEENSLGPETAGILHENLKEMLRELNR"
     gene            complement(12434..13561)
                     /locus_tag="PLZ75_01990"
     CDS             complement(12434..13561)
                     /locus_tag="PLZ75_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71201.1"
                     /translation="MKTRVKNSKLSSAGRLLPASALRLLLLVAPLSLSHGQEQGAASG
                     AKDRLNLKNEFALFESEELLDVSLEFDLKTFLRKHPKTGYQQAVLAIRISDTDTVARK
                     VRIRPRGQYRREVCSFPPVMVNFKKQLPAYSDTGKINKLKLVTHCQPGKISDENVLRE
                     YLVYKLYNVLTDTSFRVRLLRMSYIDAARTRKTIEQYGFFIEPDEIMALRTGSALVKS
                     ASLTQKMIVPSNMDRVAIFFYMISQWDWSVPGLHNIAVIIPPGHASSGKGITVPYDFD
                     LTGIVNPSYGFPDEKMGISSNRDRLYSGLCRTREEFIHALEEFRVLKENFYSVVNDFP
                     YLDQRAKKDITGFLDQFFSQLNTPREMERLVQQFMKTCKPL"
     gene            complement(13536..14537)
                     /locus_tag="PLZ75_01995"
     CDS             complement(13536..14537)
                     /locus_tag="PLZ75_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOS71202.1"
                     /translation="MKKILGIGNALVDIMTLINDDDILAEFSLPKGSMQLVDASGSER
                     IKAETKNFQKKLSQGGSIANTIHGLAMLGAGAGFIGSVGRDETGDIFENEMKKAGVMT
                     YLSRHDSPTGTAITLVSGDSERTFATHLGAAVEFNAADLDPEIFKKFDILYIEGYLIS
                     DFRLVETACRIAREQNMTIALDLASYNVVESYLNEFRTIVKDYVDILFANEDEARVFT
                     GLEPVEALAEMAGYTMISVVKTGPAGSWLRQNDEIIKVDPLPVKCEDTTGAGDLYACG
                     FLYGFAKNLGLEKCGVLGSVMAGNVIETVGTKIPGEKWPSIRKYISVLSGENSGKEQ"
     gene            14626..14699
                     /locus_tag="PLZ75_02000"
     tRNA            14626..14699
                     /locus_tag="PLZ75_02000"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14660..14662,aa:Asn,seq:gtt)
     gene            complement(14826..15491)
                     /locus_tag="PLZ75_02005"
     CDS             complement(14826..15491)
                     /locus_tag="PLZ75_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71203.1"
                     /translation="MKTKILILAMLFCSVSSLQAQQNDCKVLLESISGTYTGKCKKGL
                     AHGKGSAQGIDYYEGTFSKGLPSGKGLYRWSDGSYYDGEWKDGKKDGRGKLVRDGSEI
                     SGFWKNDEYIGKEIIPPYKVTRSLNLARSTFKRSGAIEGVRILFLQAGSENVAIENLS
                     IASSSGSDYRSGKMYGIQNVFFPLDVKIQYFTWNKMRTGKVEVIFEFTINEPGAWEVT
                     LNN"
     gene            15668..>15929
                     /locus_tag="PLZ75_02010"
     CDS             15668..>15929
                     /locus_tag="PLZ75_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOS71204.1"
                     /translation="MKKSICFLLMVLTVPVLLTGQSGKVYDNLSMKSQILKMDRKYAV
                     YLPPDYESSGRSYPVLYLLHGGGDDQTGWVQFGEVMHITDKAI"
     assembly_gap    15930..15939
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15940..16504
                     /locus_tag="PLZ75_02015"
     CDS             <15940..16504
                     /locus_tag="PLZ75_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOS71205.1"
                     /translation="MPDANTGRRGYFNSMKGDFQYEDFFFREFIPFIEKTYRIRAEKQ
                     YRAVAGLSMGGGGTILYALHHPEMFAAACPLSASVRTSSAERGRIAAADTAGLTTAQV
                     TGYLNQHNALYLIENMPDDQKTAVRWYIDCGDDDFLYEGNSLMHIAMRKKEIPHEYRV
                     RDGGHTWQYWREALPEVLDFISRAFHR"
     gene            16717..18018
                     /locus_tag="PLZ75_02020"
     CDS             16717..18018
                     /locus_tag="PLZ75_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS71206.1"
                     /translation="MTEQPSKIGHYRYRILALVFMATTINYFDRSIIGVMAPTLQNLF
                     DWSNRDYAAITVSFKIAYGLGLLFMGGLIDRLGTKIGYTMSIAIWSIFGMLHAAIKPA
                     FSLVGFMAARFGLGFGESGNFPAAIKTVAEWFPKRDRAFATGIFDASTSVGAILAPFI
                     VGAIVSVEGHRWQIPFLFTGVLSGLWVWLWWKTYHKPEFHPKLSKAELDYINSDSEPE
                     SEERIPWARTIPRRETWAFALTTVTDAVWWFYLFWGAKFLFSQFGVDIKNVGLPFLVI
                     FIMADAGSLLGGLASGALIRKGWSINKSRKITLLVNAIIILPVALVPMIASKWLAIFI
                     IGIGAFGHQSWSINSYTLVPDVFPKKATASVIGIGKMVGVAVSIVADLALGSVLDKAG
                     NSGYFWAFVIAGFSYLAILGFVHLLMPKMTPLDDNLEYITK"
     gene            complement(18090..19397)
                     /locus_tag="PLZ75_02025"
     CDS             complement(18090..19397)
                     /locus_tag="PLZ75_02025"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71207.1"
                     /translation="MASTRRNFIKTASVLAAGSVISPELFAGAGKTVAPSDKIRVALI
                     GGNSMGWSNLTSFLKNPEVECAAMCDIDRNILNKRTDELIKMGQPKPKLYVDYRNMLE
                     NKDIDIVIIGTPDHWHCLMFCDSLEAGKHVYVEKPVGNSIAEINIMQNAVRKHGKIVQ
                     VGQWQRSQPHFVDAINYLKTGKLGRIRATKAWSFVDWKGAVPKVPDSPVPAGVDYDRW
                     LGPAPKRPFNANRFHFTWRWFWEYAGGLMTDWGVHLIDYILYGMNKSVPVSVMAIGGK
                     YAFPDDAMVTPDTMTAVYDFGDFTMSWEHTIGIGLGNWRRPHGMAYTGENGTLVLDRQ
                     GWEVFPEKDRIEAVPLQKNVGIGLDLHVRNHLDCIKDNTPQKLNAGINIGRDVALVAQ
                     MGNIAFRTGEKVFWDDKRQAFTTKAANSLITPRYNNGYTLPKY"
     gene            19603..>20237
                     /locus_tag="PLZ75_02030"
     CDS             19603..>20237
                     /locus_tag="PLZ75_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOS71208.1"
                     /translation="MKINRLVIIIVLLVFIPAFSQVVILGQIRDPDSPIPPDSSVIIG
                     RLDNGLAYYIKHNKKPEQRMEMRLAVNAGSICETDDQQGLAHFVEHMCFNGTKNFPSN
                     RIVDMLEEMGMKFGEEINAYTSFDETVYMLKVPTDKTETINAGFRILEDWAHQVSMEE
                     EEIDKERGVIVEEWRLGLGADERMMQKYIPVLLKGSRYAERLPIGRMDIVKS"
BASE COUNT         5238 a         4817 c         4807 g         5365 t
ORIGIN      
        1 atggcctgcc tgtattttgc cttcagggta tcgttgaagg catacctgta taatccccag
       61 tagccgcagt aatacatttc atcgtcggaa tggttccagc tctcagacag catcctgctc
      121 caggggtcgg ctgtttccgg ggcaggaccg atttccctga acggcctcat gagattttca
      181 agataaccat gctcccgcat caggtaaaag gcagtatccc ggtatttctc cctgccggtg
      241 aaatgatagg ctgtctgaag cattcctatg atattcgatg agttcagttt cctgtcgcct
      301 accattacag gacgggcatt tacatattcc ggattccatc tgccccataa tgtcggttcc
      361 ccgttccagt cggtcagata gaaattattt ctgactatat gcgacattag ggtatcgatg
      421 agcattattg ccttctgcct gatatcaggc tggcttacca gttccgcgat ggtgccgaat
      481 gcaaaaatat gtccgatagc ctcatcactg ctggttgttg atttccagtc ccattcagga
      541 tcttctgccc gcctccaggg tatatcttcg tacttgtatc cgcgcctttc aaaggaacgg
      601 gccgggaaac ccggaacagg attgacagtg taaagccttt ccattgcatc aagggattcc
      661 ctgatgttgt ccagggcttc accggatccg cttacggcat aacggaaaac ctggccggca
      721 aggtacatgg atgtccataa accgtcatta tcagaatctg acaggcttcc tgaagataaa
      781 tccccgttta tcattccgga gagtgttgcg ttgaaaccat gccgtatatg acgctcgcgg
      841 acctgtttct cgtaaaaggc ggctttctgt tcaagggtca tctttttgaa aaagattctc
      901 cccaggcccc tgtcggtcag aatcagaacg gaattatcgg gaccttctga aatatccctt
      961 acattatccg aaggcagcca gcgttctgaa gcatagtaat catattttcc gtcctctctc
     1021 agcctgaagg cccccctgct gcttccgaac cataaactgc cgtcaatttc ctttattacg
     1081 gttatgtcag tacatggcag cctgttgtgc aattccccgt ctgctttcct gtctgcagca
     1141 tctatcagga aatatccctc accggtgccg gcaattatct ttccgcccga tgcttccaga
     1201 caggtaatat tttctcggtc aagaatcttt tcagtttttt tcagttcagg atcaaaaatg
     1261 cctatgcctt ttttccccag tatccagaaa agattattcc ggttttcaaa ttttatatct
     1321 gatatctcgt catgataaac atcttcccat aatatttccc tgtcattcag aaaagccagt
     1381 ttcctgccat cagaaacgag aaaggcaaaa tcatttcctg attcaagtat ccgtgcaccg
     1441 ggcatattgt gtttcacaaa caattttccc gcccatgcat tgctcagcag ggccgtgtca
     1501 tcgatataca gcatctggcc gaggcaggtg cctaatccgg atatcttttt atctgacaac
     1561 ggtctgtagt gatgatcctt caccagcttt cccgggaaaa gaaaccttcc tcccgagggt
     1621 tttaaaagtc ctgcagagga aaagacctgt atatagccgt tccggtccga cactacattg
     1681 tgaaatactg tcttttcatc atcagccggg tatttaatgc tgtagtcctg cacgaaggga
     1741 agatcccggt gatgctgaac cggacgggga tgtttgttat tactgcaggc tgccgtacag
     1801 atgattatgc acagggcagc tgaaatggca taaattgttt tcataggaag ggatctgtca
     1861 ggctaaaagt tttacggcat tttcataaat tttccttact gcatctgcaa acatctccat
     1921 atcctttctt gtccccatca gaaccttgtg atgcacacat accgattccc cggaatgaat
     1981 cctgtcgcat accggcagat caaagcgtga agggtctatt gccttccagt attcttcgct
     2041 tagtctgtgg cgcggagctt ttgtacaggg tgcataaagg gaacatttgt tcagggggat
     2101 atagcttgcc gcgatctcaa taccgagttc agcggaaagg gcctgtctga acttttcagc
     2161 cggaagatca ctgaaagcct tgctgttgta tctgaaagcg aaattataat aggccccggc
     2221 tgtctggcgt tcatcccttt tcacaggctt tatgccggga agcttttcca ggagggaatt
     2281 taaatacagg gcattttctt ccctcctttt attttgttca ggcagccgcc tgagaccctc
     2341 aataagaatt gcagcctgga attcagttat cctgtagttg ccggattgca ggaagttacc
     2401 tgcatcgaag taaaaccccg cccccttgtc ggttttatca aaaccggccg gttccggtct
     2461 cctgccgcag ttgcgcaggg cgtcaagctt ttcatataaa ctgaaactgc tcgtggtgag
     2521 agctccgcct tcacccgcgg taagcagctt tgacagctga aagctgaaac tgccgatatc
     2581 tcctatgctg cccgcctttt tccccttcca ttcccctccg tgcttgtgtg cacaatcctc
     2641 aataacaaaa agaccatgtt ccccggcaat tttcattatt tcgtccatat cagcaaagga
     2701 accgtagaga tgaaccggaa taattgcctt tgtccgcggg cttatcgctt cacggatctt
     2761 ttccggatca atgcaccatg tatcctcgca tacgtcggca agaaccggaa gggcattgac
     2821 atcaagaaca gttgcagctg tcgcctgcca ggtaagcccc ggaagaatca cctcgtcgcc
     2881 aagcccgata ccgcaggctt caagtgccag ttgcagtgtt accgtcccgt ttgcggcaga
     2941 caatgcatat tctgttccgt tatagtcagc aaacatctta ttgaactgat tttccagcgg
     3001 tccgttatat gaccagatgc cgcttctgag ggtttttatc agtgcctttt cctcgttttc
     3061 atcccataca ggccactcgg gccagggcat tgtcctggtg tcccgtacgg gagtaccgcc
     3121 aagaacagcc ggtttatctg agaagttata attcattccg gtataaatag tgaaaagtga
     3181 atgatccaag ataggataaa ataaaaacaa agtaaagcgt aattgcaggt tttaagttat
     3241 acgcagatga aactacagcg gtggtgatat caggcatttc catgttcaga gctgtttaat
     3301 gcctgaatca tttatcataa ttcttggatt tttagaataa aaatccgaaa ttaggagtta
     3361 acttccttat gatcaacctg acgtactctc atgcatggcg tgctccatac caatatcatc
     3421 actattcccg gacagattca ctggccggat aatcagaatt tatcaaatag gaaattctat
     3481 gaaaaagttt tgttctgttg ttttaattgc atttgcaacc ggaccggtcc ttctggtatc
     3541 ctgtaagaaa gaagagccga agggtcctcc ggtagcaggg attttttaca gtgttgtcga
     3601 aaagaaagtg gcatttacag cccttaccaa gagggctgac acctggctgt gggatttcgg
     3661 cgacggacag acaagcactg aaatgaatcc ggttcatatt taccaggaag gtggtatata
     3721 tgatgtgaag ctcacagtga ccggcagtgg tcagagtgcc ggggcaagca cggttgtctc
     3781 gcttgccctt tcaaacatcc agatgcttac cggcggcctg aaatatcctg acggcaagaa
     3841 atggaagata agcagcgctc actccgataa agatgcattt acactggctg atgacaagtt
     3901 taccgttata cagcctgtgg ccaccggcat gcttggaagt gtagggcttg gccttggtga
     3961 agtgtatgat gatattttta tcttcaaaca tgacggatcc tatatccatt ctccacagca
     4021 tggaggagct tttgcaggat tgctttattc aatgcttaca aagcagaata tccttaatgt
     4081 cacacctgtg agccaaagtt tcggtttatg ctatacagca tggacccccc agacagcaac
     4141 attcaccctc aatgagaaga aagatctgga aatcactacc gtaagtccgg atggtaatac
     4201 tccggctgac cgggtttata aagatgtcat ggctctgagt ttttcaggta ctgaatttat
     4261 cggattcatg gattttacgc gcgagtgcat aatccagtcc ctttcaccct caaaaatgag
     4321 acttgcaatg ttcgtatgta caacacaaaa ggctcatttt aacaaacctt ctcatgttgt
     4381 gatcctgaca tttgaaacag ttccctgaac agttacttaa acctgtggaa ataaaaaaca
     4441 aaaacatata atgatgaaac atcatatcaa aataaatgac attgtccggt atgtgatacc
     4501 ggcaatgttg ctgtcttttc tgctgacgca caatcttatg gctgaccgga ttccggatta
     4561 tggcataaac ccgggaagca gtattgccgg gcagcaggga attgtaatca ccggccgggt
     4621 aactgcagaa actgataacg cactgatgcc gggtgtcagc atagttgtca gggggactcc
     4681 caggggtacg gtaacgaatt ctgccgggga atactccatt gaggttcccg gaagcgatgc
     4741 cgtgctggta ttctcattca taggatacac tacggaagag gtcaccgtgg gcagccagag
     4801 aaccatcaat gttgtcctga gggaatcagt agaggcactg gaggaagtgg tggtaacagc
     4861 cctgggtatc accagaaggg agaaatccct cggatattcc gttggcaggg tgagcgggga
     4921 agatctggcc cgggtggttc aggagaatgc aataagttcc cttgccggga agatgaccgg
     4981 cgttcagatc agctccacag gcggtaccgg atcttcggta agcatgatta tcagaggggc
     5041 tacgtccctg agcaatgata atcagcctct cttcgtgatt gacggagtgc cggtcgtaaa
     5101 taccctgaac aatatagcag gtttcggaag cgataaccgt gtggattacg ggaatgccat
     5161 ctccgatctt aattccgatg acattgaaag tgtttccgtg ctgaaggggc cgagtgccgc
     5221 tgctttgtac ggatcgcggg caggaaacgg tgtggtaatt attaccacca agtcgggaat
     5281 gaagagcaag ggcatgagag ttacaataag taccaacaat gtgttcgaca agccttacaa
     5341 gtatttcccc gttcagaaaa agtttgcttc gggctatttt tcatttacac ccgaagatct
     5401 gcctccggga tatgtgatga aagtcaatcc ggccgaagcg gcaggcgcgg ggattgaact
     5461 cgacaaggaa tattttgctg ttaagtggga cagcccaagg gatgcaaacg gtgtccagat
     5521 accggtggaa ctggtcagcc atccagacaa cctgcctaat tttgtacaga ccggaataac
     5581 ttccacaaat gatatatcgg ttaccaataa taccgatatg atgaattaca ggataggagt
     5641 atctaacatg tcaaacaggg gaattgtccc gaattccgat cttttcagga ataatcttac
     5701 agccgcaact tccctgaggg taagggaaaa cataaccctg agctcaaata ttaacattaa
     5761 ccgctcatgg tccaataaca gaccggcggg caacaggggg gccaatccgc tagaatgggc
     5821 ctataagata ccacaggaca cagatataag agatctgagg aattactggg aagaaggtca
     5881 ggaaggcatt cagcagagaa ctccctataa cggaatttac aacaaccctt atttccttgc
     5941 atacgaaata aacaacagtt tcctgcgcga caggatattc ggaaacctta aggccgaatg
     6001 gaaaatatca agggatttta cccttatggg aagatattcc cttgaccagt tttccgagaa
     6061 acgggaataa aaactggctc ccagctatac aaaggaatcc aacaacggcg cctacggcgt
     6121 tcaggattta accagctatg agaggaacgc ggatgttctg gcaacctatt cgaaaaccac
     6181 ggataatttc agcctgtcag cctctgccgg tggcaatgct ctctataaaa aaggaacatc
     6241 tgtaagcagt tcatcaaaat cgggtgcagg cctgactgtt cccaatgttt ttaccctgag
     6301 caacattaaa tccggatcac tcgattactc gagttactgg tcccagaagg ccatttacag
     6361 catatacggc ctgatcaatt tcgggttcag ggatatgata ttcctggata tcacaggacg
     6421 gaacgactgg tccagtacgc tgccccggga aaactggtca tatttctacc cctctgcttc
     6481 actgagtatt atagtaaatg aaatgctgaa catgggtggc ggagtggagc ttttcaagat
     6541 aagaggcggc tgggccaagg tgggtaacga caccgatccg tatcagctgt tcagtactta
     6601 cggaaatgcc ggtcagtggg gggatgcaac ccgcctgaca aaatcgggac agatacttaa
     6661 ccccaacctg aaacccgagg aagctgtttc cagggaagcc ggcctggacc tgaacctttt
     6721 cagaaacagg ataaggtttg aaggaaccta ctatgtcgtg gagaaccgta accagataat
     6781 caggaatatt cccgtggcca gttccacagg atcagataat gtgaatataa atgccggact
     6841 tataagaagc aagggctggg aattcatgct gggcggtact cttctgaaaa ctcccggctg
     6901 gtactgggat gtaagtgcca atttttcaag gaacaggaca agggtaattg aaatagcaga
     6961 gggaattgat gtcattaagt tctgggaaga tgccagggga ggagcctggt cctacgtggg
     7021 ggatgaaata ggcgcaattt atgatgccga ggttattacc gtgaccgata aaaattcacc
     7081 ctactatggt tacccgatac ttgatgaagg tgacctggaa tggggagcag tcgagatgcg
     7141 atataccaaa aacaagatag gaaactataa tcccagattc ataatgggat tccggagctc
     7201 tctttcctat aaggcattct ccctgaatat gactgtcgac tggaggaacg ggggccagtt
     7261 catttcccag acgcaaagat acatggctga ggacgggagt tcccagatct ggctcgataa
     7321 ccttatccat cccgggggaa ggacgggcag agaactgcgt gactggctcg ttcagaatga
     7381 ggacaagtac atcaggaacg ggttccatgt tgtcggcgga ccgactaagg aatacggggg
     7441 cttccgtgaa aatttcagcg gcgtatatgt aaatgacgga atatttgttc cgggtgtatg
     7501 ggcgaaggat gacggtgagg gaggtgtgac ctatattgag aatctgggag aagacgggac
     7561 agtgatagta ccctatgtgg taagctatcc gtggggattc gccaaacctt ccatgtttga
     7621 ttccgactgt gtgaagctga gggaaatctc tttgagctac agtgttccgc gggacgttgt
     7681 aaacaggctc cgcttcatcc atgacattgc cctgtcgctt tacagccgca atattatact
     7741 ctggacaaaa gccaaatccg gaattgatcc ggaaagggca tttcaggcag aaagctcaac
     7801 aggtggcacc aggggaacac aattcaagca gggtattgaa agattcaatc tggaaccctg
     7861 ggtaatcccg gtgggaataa aactggacat aacattctga ttaaaaacag aaattatgaa
     7921 acccgcagga taatctgtaa taacgcgggt ttcaccatac cattagaatt aaaatcatat
     7981 tatgatgaaa gcaataaaac ataaaatctc agcgctcctg acagtcctgc ttctgatgtt
     8041 tatttcctgc cagaatctcg aagagctcaa cataaaccca aatggtgttg atccggcaaa
     8101 tgcccggccc tttttactga tggctaccgt gctgacccag accggtcaga atgtggtggg
     8161 actgggtttt ggcgatctgg ccggcgttat gcagcatacc cagaaggacg gatgggccgg
     8221 aggccataat tcctacgact ggtcagatca gaactggagc agtttctacg ggatattgag
     8281 aaatgccgag gagatcctga aaaagtccga aagtgacgga cttgaattct acaagggtgt
     8341 tgcactgata atcaaggctt acaattatgg aatgatcaca gacctgtggg gtgatgcccc
     8401 cttctccgag gcattgaagg gggaggcagg gaatctgaaa ccctcatacg actcccagaa
     8461 ggatatttat acgggtattc tcgcctggct tgagactgca aatacattgc tgtcaaaaga
     8521 ccagaactct tatccggaca tcaataccac ccaggatgtc atgtacggcg gttcggtcag
     8581 caattggaga aaatttgcca attcacttgc acttcgctat tatatgagga tttcctcgaa
     8641 agaaccttcg gttgcaagaa cgggaataga aaagattgcc gctgatccgg aaaaatatcc
     8701 gctgattctt gatgccaggg atgacgcaaa tatagcctat gtagggaaca gcacctcaga
     8761 ctcgtggccg tgcaacacca cttttgacgt cagcgagtca aactggagaa gacttaaaat
     8821 gtgttcaacc cttgttgaaa agcttcagga actcaatgat ccgagacttg ccctctgggc
     8881 cagtaaaatt gctgttcccc tggttcttga tccggacagg cctgacgatt ttgatgaaat
     8941 aattgatggc aggcgggtca tagctcagaa tattgccgat acctatgagg caacctatga
     9001 cgttctcctc aacctcgatc cggaatatgt gggacttccc ccggcatggt caatactgcc
     9061 gcaggcatac aatctatgtc ccaatcttga acaggccccg ctgaatccgc atgcctcaca
     9121 tctcagcaac atgtataagc aggcagccgg accacatctg aaggcacgcc tcctttcggc
     9181 cgcggaggtc aatttcattc ttgccgaggc tgcgctgaac ggatgggctg caggcggctc
     9241 tgcaaaggat tattatgaag ccggcataag agcctccctg gatgcatggg agcttacagg
     9301 atcatatcag gcttatatca ctgaagacgg ggttgcctgg gacggaaccc agggccagct
     9361 gatggggcag aaatggattg caggctggac ggcagcggcg gaatcctggt ttgactacag
     9421 aagaaccggc tatcccgaac tgttgccggg aaagatagtg aaaaggagtg ctatccccct
     9481 gcggttttat tacgggacaa acgaattgct gtataacgcg gaaaaagcag aagctgccat
     9541 tgagagactt gaaaccacaa gtttttcatc aaccgacggg aaaaacagcc cgtggtcaaa
     9601 gtcgtggctg cttcagggta ccggaaaacc atggtgaccg gataatatgt taatgccccg
     9661 cggacagaag gatgcttttg gcctgattct gcctgcaggg ctgcagacct ccggacttct
     9721 gacttccgga tctcctgacc ggattatttg agatactgaa ggaaaaagtc attcattttt
     9781 tcctggcagt aaatatttac cctctgaaca atatccatgg tatggggcca gccggggata
     9841 cggtggtaaa cacagggtac tcctgccgaa tcgagcagtt ccctgaaacg gtccgactgg
     9901 cttacgggaa caagacggtc ggatgtgccg tgaaaaatca gtgtgggagg atcattcccg
     9961 tcaatataac tgaccggtga tgcttccctg aaaatatccg gattttcatc ccagctgctg
    10021 ttcaggaatg atgtcacaag cctgtggttg cgggcatatt ccgttgtgag atccaccggt
    10081 ccgtaaatgt cggccacagc ctttatcttc cccctggttt tttgtccggg aattgtgtca
    10141 tgaagaacag cgggtttcca tccgtaggct gcgagcagtg ccagatgagc tccggctgaa
    10201 cccccggcca gagccatacg cgaagtatca taaccgtatt ggtcaccgtt ggaatggaac
    10261 catattaccg catcggctat atcttctacg caggccggat agggagcgtc cctgagcagc
    10321 ctgtaggata cagtcgccgt aacatatccc tttcgggcca agccaataag ataaggcaga
    10381 taatctgatc ttttgccgct tctccatgag cctccgtgta taaaagcaat aagcggggca
    10441 gggcccgggg aatttttatc ccggtaaata tccagctgca gggacctgcc attgatattt
    10501 ttgtattcaa tatccctgaa ttcctcaatg ttttccggaa tttcaggccc caggtccatc
    10561 agcttttcaa cacccagcca tactgcagca taagttgccg gagtaaaaaa ataacccgcc
    10621 ggcggctttc ccggactaag agatgaccaa agccgtggaa ggctcatcca aagaacaaaa
    10681 accactataa ctgctgccag agccagcagg cctgacctga caatacgttt aagcaggctg
    10741 tatttactac ggatcgccat aagtcagcgg ttcagctccc ggagcatttc cttaaggttt
    10801 tcatgcaata tccctgccgt ttccggcccg agggagttct cctcaccgta aggcgggcta
    10861 ttcctgccgt aggtgtaggg tgatttcaca tcccactgac tttttggaat aacgtagcct
    10921 atttcatcat ttgccaggcc gaaaaagaat ttgaaccttc ccggcatcat ttctctaacc
    10981 ggaggcagct ccgccggccc ggtggaaaag tcattcccct ccggagcttc aatacctccg
    11041 ttgacaattt caggatatac ttcccccggc atggtggcaa atgacagcgg acctatttta
    11101 aaaaccgcca gttcggttcg cattttcatc cacccggtca tgccacggtc aaggattccc
    11161 agcatcgttc ccagtctgaa caatttattc ctgacgggaa ggattaccgt tctggcaaca
    11221 agtgaaagag tggcggaatc tatccttgcg gcaggatttt gcatggcctt cagtgccaga
    11281 agggcaacct gctttccctg ggcttcggct ttcgcaaatg agggctctga atattcagta
    11341 tccgaaaacg ggtcttttac actcagactc ggatgtgtgg tcatcagtcc tcctattgca
    11401 ccgtttacat atatggccgt cccccccgtg ccttccatac cctgattgcc acctccggaa
    11461 acagtttctt ccgttcctat gccggcaagg ctgctgcccc gggaaaatcc ggcttctgtt
    11521 ctttctccgt cagtgctgcc atttctgaaa acaccctttt ccacaccttc cctgacgaaa
    11581 tgagggaaat cagaggtaat caggagatta tttccccaca gtgtttccgg atgatccgcc
    11641 caggatatga ctgaacccag ggtacttccg ttttctgcgt caagcacttt gatgcagcgg
    11701 agcccggaat catatacatc gggtttcctt gtgtcggtta ccaggtgctc agcacctgtc
    11761 agatcttccg atacctccag gtatgcaggg cgcagacttt ctgttgcggc aatcaccgat
    11821 tccactatac gcctctttac atattcctta tatttcccgt ccactccgct tttcaggggt
    11881 gattttcccc aaagtcccag catgtcagga ccttcatgtg tatgggtgga aatgattatt
    11941 gtgtatgtaa tgccggcatc ctcaggtatc ctgcttctga catctataac gtcattgttc
    12001 atgaaaccta tcagatccag aacaactatt gccagcctgg atttcccgtc atccagtatc
    12061 atggttctgg cccagaggtc gtcatgcact ccgtttgccg cccttttgtt accgaatcct
    12121 gcaatccaga cgggatcgaa aacgccgttt ccgttgccat cagtaaaggt atcccccctg
    12181 cggggttcgt actttgcatt gttatctgca tcagtccagc ggtcaggaac ctccggagtg
    12241 atgctgacag cagaaaaacc tgctttaagc gcggacactt ttcccccgtg tatttcaata
    12301 tccgccctgt agcccggatt atggtccttc atgtttttaa aagacaagac aattatggcc
    12361 agaattactg caagcagggc aattcctgct gccttgaata tgatcttcag ccttttcatc
    12421 tgatattcct gttttataga ggtttgcaag ttttcatgaa ttgctggacc agtctttcca
    12481 tttcccttgg tgtattcagc tggctgaaaa actgatcgag aaaaccggta atatcttttt
    12541 tggccctctg gtcaagatac gggaaatcgt tgaccacaga ataaaaattt tccttaagaa
    12601 ccctgaattc ctccagggcg tgaataaatt cctccctggt cctgcacaag ccgctgtata
    12661 atctgtccct gtttgagctg attcccattt tttcatcggg gaaaccgtag gagggattta
    12721 caattcctgt gagatcaaaa tcatagggaa ccgtaatgcc cttccctgag gaagcatgac
    12781 cgggaggtat tatgactgca atattatgaa gacccggtac tgaccagtcc cattgtgaaa
    12841 tcatataaaa gaaaattgcc accctgtcca tatttgaagg gactatcatt ttttgtgtga
    12901 gactggctga cttaaccagg gctgatccgg ttctgagggc cattatctca tcaggttcaa
    12961 tgaaaaagcc atattgctca attgttttcc tcgtcctggc tgcatcgatg tagctcattc
    13021 ttaacagtct taccctgaat gatgtatcag taaggacatt ataaagttta tatacaaggt
    13081 attcgcggag aacattctca tcggatattt ttcccggctg gcaatgtgtc accagtttca
    13141 gcttattaat cttaccggta tcggagtaag ccggaagctg ttttttgaaa ttgaccatta
    13201 ccggggggaa actgcatact tcccggcggt actgccccct gggcctgatt ctgactttcc
    13261 tggcaacagt gtcagtgtca gatatgcgta ttgccagtac cgcttgctga tatcccgttt
    13321 ttggatgctt cctgagaaat gttttcagat caaactcaag gctgacgtca agaagctcct
    13381 cgctttcaaa aagtgcaaat tcatttttca ggttaagcct gtcctttgcc cctgaagcag
    13441 ccccttgctc ctggccatgt gacagtgaga ggggagcaac cagaaggaga agccggagtg
    13501 ctgacgccgg aagcagtctt cctgctgaac taagtttact gttctttacc cgagttttca
    13561 ccgcttaaaa ctgaaatgta ttttcgtatg gaaggccatt tttcgccggg gatctttgta
    13621 cctactgttt ctatcacatt tcctgccatt acggacccga ggaccccgca tttttcgaga
    13681 ccgagatttt ttgcaaagcc gtacaggaat ccgcaggcat aaagatcgcc tgctcccgtt
    13741 gtatcctcac attttacggg aagcggatcc actttgatta tttcatcatt ttgcctgagc
    13801 catgaaccgg ccggacctgt cttcacaact gaaatcattg tgtacccggc catttccgcg
    13861 agtgcctcca cgggttcaag tcctgtaaaa acccttgctt catcttcatt ggcaaagaga
    13921 atatcaacat aatccttcac aatggtcctg aattcattca gatagctttc aaccacattg
    13981 tagcttgcca ggtcaagggc gattgtcata ttctgctcgc gggcgatcct gcaggcagtc
    14041 tccacaagcc ggaaatcgct tatgagataa ccttctatgt aaaggatgtc gaatttttta
    14101 aatatttcag gatccagatc tgcggcgtta aattccactg cagccccgag atgggtggcg
    14161 aaagtccttt cactgtcccc tgagacaagg gtaatggctg ttcctgtggg agaatcatgc
    14221 ctcgacaggt aggtcatcac acctgccttt ttcatttcgt tttcgaaaat atctcctgtt
    14281 tcatcccggc cgacagaacc aatgaatccg gccccggcac ccagcatcgc cagtccgtga
    14341 atggtatttg caattgagcc tccttgagag agtttttttt gaaaattttt tgtttcagcc
    14401 tttattcttt ctgatcctga tgcatctaca agctgcatgc tgcctttcgg aagtgaaaat
    14461 tcagccagga tatcatcgtc atttatcagt gtcattatat ctacaagggc gttgccgata
    14521 ccaaggattt tcttcataat taaaaatttg cttaaagata ttgttaaatt cactaaaagc
    14581 ccttattttt ggagcgctgg ttaaaaaatg gcaatcagca aaaattcctc agtagctcag
    14641 acggttagag cggcggactg ttaatccgta ggtcgcaggt tcgatccctg cctggggagc
    14701 aaaacccggg aaaatcagat aaatataacg gcatcatagc tttgggttcc atcccgggcg
    14761 cggagcctca tgcaggtacc ccgaattatc cgtgaagccg tcatccggag ccatttcccc
    14821 ctgtattagt tattcagcgt cacctcccat gcgcctggtt catttatggt aaactcaaaa
    14881 atgacctcca cctttccggt ccgcatcttg ttccatgtaa aatactggat cttcacatcc
    14941 agcgggaaga agacgttctg aatcccgtac atcttgcctg aacggtaatc ggacccgctg
    15001 gaggaggcaa ttgacagatt ctcgatagca acattttccg agccggcctg aaggaataaa
    15061 atccttaccc cttcaatcgc accggatctc ttaaaggtgg atcgtgccag attcaggctg
    15121 cgagttactt tgtaaggagg aataatttcc ttgccgatat attcgtcgtt tttccagaat
    15181 ccgcttattt ccgaaccgtc cctgacaagt ttccccctgc cgtctttttt cccgtctttc
    15241 cattcaccat catagtagga accgtcactc catctgtaaa gacctttgcc tgaaggcagg
    15301 cctttggaaa aggttccctc gtaataatca atgccctgtg cgctaccctt tccgtgagcc
    15361 agcccctttt tacacttgcc tgtataggtt cccgagatac tctcaaggag caccttgcag
    15421 tcattctgct gtgcctgaag tgagctgaca gaacaaaaca acattgccag gatcagaatt
    15481 tttgttttca tatgaatctg tattaattaa aaaaagaatg tcagaagaca tgttttattg
    15541 actgcttttc cattcataca aaattactac tctttccatc atttcaatgg atattatatt
    15601 ttgatatttt aactttgaat catgtcatta attcaataat attgaatgaa taaaataatc
    15661 agcgaaaatg aaaaaatcta tctgtttttt gttaatggtc ctgactgttc cggtcttgct
    15721 gaccggccag tccggcaaag tgtatgacaa tctttcaatg aagagccaga tactgaagat
    15781 ggaccgtaag tatgcagttt atcttccgcc cgattatgaa agctcgggcc gcagttatcc
    15841 ggttctctac cttcttcacg ggggaggtga tgaccagacg ggatgggttc agttcggcga
    15901 ggtaatgcat attaccgaca aggcaatcan nnnnnnnnnc atgcctgatg ccaatacggg
    15961 ccgcagggga tacttcaatt ccatgaaagg ggattttcag tatgaggatt ttttcttcag
    16021 ggagttcata ccctttatag aaaagaccta caggatccgg gcagagaagc agtaccgggc
    16081 agtagccggt ttgtccatgg gaggaggcgg aacaatcctg tatgcacttc atcatccgga
    16141 aatgtttgct gcggcatgtc cgctgagcgc ctcggtacgg acaagcagtg ccgagagggg
    16201 cagaatcgct gcagctgata cggcaggtct tacaacggca caggtcaccg gttatctcaa
    16261 ccagcacaat gccctgtatc ttattgagaa catgccagat gatcagaaaa ctgcagtgag
    16321 atggtatatt gactgcgggg atgatgactt tttatacgag ggaaattcac tgatgcatat
    16381 tgccatgcga aaaaaggaaa tacctcatga ataccgggta agggacggcg gacatacctg
    16441 gcaatactgg agggaggccc tgcccgaggt cctggatttt atatcaaggg cattccacag
    16501 gtaatacccc tgcttcctga aaggtcagag aaaaatactg ccggtccggt aaaaatacag
    16561 ccggtcaggg aaaaataccg ccggtccgga ggggggtaat gtcagccggc agcagaaggc
    16621 tgtgtcatct gaaaatgaaa agtaccagtt ttcaggccgg ttttctttat tccgatattt
    16681 aattattttt atggcagata accttattac ttttccatga cagaacagcc ctcaaagatt
    16741 ggtcactacc ggtaccggat acttgccctg gttttcatgg caaccaccat caactatttc
    16801 gaccggagta ttatcggggt aatggccccg acgctgcaga acctgtttga ctggtcaaac
    16861 agggattatg cggcaataac tgtatccttc aagattgcct acggactggg attgcttttt
    16921 atgggtggtc ttatcgacag gctcggaacg aagattggct atacaatgtc cattgccatc
    16981 tggagtattt tcgggatgct gcatgccgca ataaaaccgg ctttcagcct ggtgggattc
    17041 atggctgcaa ggtttggcct tggtttcggt gaatcgggaa atttccccgc ggcaataaag
    17101 actgtggcag agtggtttcc gaaaagggac agagcctttg caaccggtat atttgacgca
    17161 tcaacaagtg ttggcgccat ccttgccccc ttcattgtgg gagccattgt aagcgttgag
    17221 ggccaccgct ggcagatacc cttcctgttt acaggcgtgc tgagcggttt atgggtgtgg
    17281 ctgtggtgga agacatacca taagcccgag tttcacccga agctgtcaaa agccgaactt
    17341 gactatataa acagtgattc cgaaccggaa agcgaggaaa ggataccctg ggctaggaca
    17401 attccccgca gggaaacctg ggcctttgcc cttacaacag tgactgacgc cgtatggtgg
    17461 ttctaccttt tctggggtgc aaaattcctt ttctcacagt tcggagtgga tataaaaaat
    17521 gtgggactgc ccttcctggt gatttttatc atggctgatg ccgggagcct gcttggagga
    17581 ctggcctccg gtgcgctgat aagaaaagga tggtcgataa acaagtcacg gaaaatcacc
    17641 ctgctggtca atgccattat tatactgcca gtagcccttg ttcccatgat agccagtaaa
    17701 tggcttgcca tttttataat tggcataggt gcattcggac atcagtcatg gtccatcaac
    17761 agctacactc ttgtgcccga tgttttcccg aaaaaggcaa cggcttcagt tatcggaata
    17821 ggtaaaatgg ttggtgtggc tgtttcaatc gtggcggatc ttgctctggg ctctgttctg
    17881 gacaaggccg gcaacagcgg ttatttctgg gcattcgtta tcgccgggtt ctcatacctg
    17941 gccatactgg gatttgtaca tctcctgatg ccgaaaatga cacccctgga tgataacctg
    18001 gaatatatta caaagtaggc ttatcgatgc tgaatgaagg tccgggaaag atattccgga
    18061 ccatggtatc atgaaaactc cgcttcctcc taatatttgg gaagggtgta tccgttattg
    18121 tatcttggtg ttataaggct gtttgctgct tttgtggtaa aagcctgtct tttgtcatcc
    18181 cagaacactt tctcgccggt gcggaaggct atattgccca tctgtgccac cagtgccaca
    18241 tcacggccga tattgatacc ggcattcagc ttctgggggg tgttgtcctt aatacagtcg
    18301 agatggtttc ttacatgcag gtcgagacct attccgacat ttttctgaag cggaacggct
    18361 tcaatcctgt ccttttcggg aaatacttcc catccctggc ggtcaagcac gagcgtgccg
    18421 ttctcaccgg tatatgccat accgtgaggt cttctccagt tccccagccc gatgccgatg
    18481 gtgtgttccc acgacatggt gaaatccccg aaatcatata ctgccgtcat ggtatcgggt
    18541 gttaccattg catcatcggg gaaggcatat ttacctccta tggccatgac ggatacgggg
    18601 acagacttgt tcattccgta aaggatgtaa tcaatgagat gcacacccca gtctgtcatc
    18661 agtccgcctg catattccca gaaccagcgc catgtaaaat gaaagcggtt tgcattgaag
    18721 ggccttttcg gggcaggacc gagccatctg tcatagtcaa caccggccgg gacaggcgaa
    18781 tccggaacct tcgggactgc tcccttccag tcgacaaagg accatgcctt ggtagccctg
    18841 atacgcccaa gcttaccggt cttcagataa ttaatggcat caacaaaatg cggctggctt
    18901 ctctgccact ggccgacctg tacaattttt ccgtgctttc ttacggcatt ctgcattatg
    18961 tttatctcgg caatggaatt tcctacgggc ttttcaacat acacatgctt gcctgcttcc
    19021 agtgaatcac aaaacataag gcagtgccag tgatcgggag ttccgataat tacaatatct
    19081 atatccttgt tttccagcat attccggtaa tcgacataaa gcttcggctt tggctgtccc
    19141 attttgatca gctcatcggt ccgtttgttg agaatattcc tgtcaatatc gcacatcgcc
    19201 gcacattcaa cttccgggtt tttcagaaat gaggtaaggt tactccatcc catactgttt
    19261 ccccctataa gggcaaccct tattttatct gatggtgcaa ctgtttttcc tgctccggca
    19321 aacaattcag gagagatcac ggaacctgct gcgagtactg atgcagtctt tataaaattt
    19381 cttcttgtcg aggccataac tgatgatatt tcgggtaata agccgcaagt taatattaat
    19441 attgcttaat tatcacttca tagcgttaaa aaatctgaaa tgtaccgggt ctgctacgtg
    19501 accgggaaag gtttaaaaag tatttgtaag tctgtttttt gattcacgaa actggaatta
    19561 gttcgcttta aatactacat ttaccaataa atatgatatg aaatgaaaat aaatcgactg
    19621 gttatcatta ttgttctgct ggtttttatc ccggctttta gccaggttgt catccttggc
    19681 cagatccggg atcctgacag tcccattccg cctgattcca gcgttatcat cggaaggctt
    19741 gacaacgggc ttgcatatta catcaaacac aataaaaagc ctgagcaaag gatggaaatg
    19801 aggctggccg tcaatgccgg ttccatttgt gaaacggatg atcagcaggg acttgcacat
    19861 tttgttgagc acatgtgttt caacgggaca aagaacttcc ccagtaacag gatagttgac
    19921 atgctcgagg aaatgggcat gaaattcgga gaagaaataa atgcatacac cagctttgat
    19981 gaaactgtgt atatgcttaa agtgccgact gacaagactg aaaccataaa tgccggattc
    20041 cggattcttg aagactgggc tcatcaggtt agcatggaag aggaggagat agacaaggaa
    20101 aggggtgtta ttgttgagga atggaggctc gggctgggtg ctgatgaacg gatgatgcag
    20161 aagtatatac ctgttctgct gaaggggtcg agatatgccg aaaggctgcc cataggcaga
    20221 atggatatag taaaatc
//
LOCUS       DAOSPX010000020        19965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2882_length_19965_cov_4.408237, whole genome shotgun sequence.
ACCESSION   DAOSPX010000020 DAOSPX010000000
VERSION     DAOSPX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2882_length_19965_cov_4.408237"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLZ75_02035"
     CDS             <1..481
                     /locus_tag="PLZ75_02035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HOS71209.1"
                     /translation="CITGNFPYNISSQILFRVLEYRNKVTEVCGMFQKEVAERICSPP
                     GSRVYGILSVLLQAYYHTEYLFTVPGEVFSPPPKVNSGVVRLVRNDVKELEVNETLFR
                     QVVKAAFSQRRKKLRNSIRSAFALRSEDCPLFDLRPEQLSVNMFADLTKWIEENRVV"
     gene            complement(499..1752)
                     /locus_tag="PLZ75_02040"
     CDS             complement(499..1752)
                     /locus_tag="PLZ75_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS71210.1"
                     /translation="MKYLKLLKESVILALGSVMINKLRTFLTLSGITIGIFSIISVFT
                     VLDWMEKAIRDSISSMGSNVVYIQKYPWSMGASLKWWDIIRWPAVALDDYHAIAGRST
                     RTESLSLVVGHSASLSYRNNSAGGAAVAAVTDNLEKVLSFEIEKGRYFSPYEYTSGTN
                     VAVLGAETAERLFKDENPLGKEIKLQGRKVRVIGILKKEGEGGINLSNIDQITIIPLN
                     FGKNFINLRNRFVNTQLIVKAKPGVNVQELSDEATMILRSARRLKPVEPENFSVNTPT
                     MLSKGFDPVFTGINIGGWLIGGFSILVGGFGIANIMFVSVKERTNMIGIQKALGAKRS
                     FILQQFLAESVLLSVIGGIFGIFLIFIGTIAVNYLYELNMHLTFSNIMLAIVISGVIG
                     IIAGYAPAYSAARMNPVDAIGYSFS"
     gene            1940..3463
                     /gene="purH"
                     /locus_tag="PLZ75_02045"
     CDS             1940..3463
                     /gene="purH"
                     /locus_tag="PLZ75_02045"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOS71211.1"
                     /translation="MSERKIKSALISVYHKEGLDDIVSLLNQLKVRIFSTGGTWDHIT
                     RTGIRAEKVEDLTTYPSILGGRVKTLHPSVFGGILARRENSGDMEQMDRYGIPEIDLV
                     IVDLYPFGETVRNTDDEAEITEKIDIGGISLIRAAAKNFRDVVVISSRDQYSRLLELL
                     RGKGGMTSLQERRRFAAEAFMNSSHYDTEIFRYYNLREKLPVFRESINISGELRYGEN
                     PHQKGIYYGDPGEMFEKLHGKDLSYNNLLDIESAVTLIDEFTETTVIIIKHNNACGAA
                     SRDNLTDAWNDALACDPVSAFGGIIAANREIDEQTASAMDKIFFEVLIAPGFSAKALE
                     ILEQKKNRIILKRKAVFRMEAAFRSLLNGVLWQERDNSTQTPEDLMPVTPRKPDKNET
                     EDLVFANILVKHSKSNAIVLAKNKQLCASGIGQTSRVDALKQAIEKARLFGFDLKDSV
                     MASDAFFPFPDCVEIAHKAGVTAVVQPGGSVRDKDSIDFCAANGMAMVFTGIRHFKH"
     gene            3474..4496
                     /locus_tag="PLZ75_02050"
     CDS             3474..4496
                     /locus_tag="PLZ75_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOS71212.1"
                     /translation="MGLFSFLTQEIAIDLGTANTIIIQNDKIVVDEPSIVAIDKNTGK
                     LIAIGEAARQMHGKTHENIKTIRPLRDGVIADFNAAELMIRGMIKMINKGPKLFSPSM
                     KMVICIPSGSTEVEIRAVRDSSEHAGGRDVYIIYEPMAAAIGIGIDVEAPEGSMIVDI
                     GGGTTEIAVIALGGIVCNKSIRIAGDGFTADIQAYMRHQHNIKIGERTAEDIKIAVGA
                     AFPDIDNPPPDYIVRGPNIMTALPIEIPVSYQEIAYCLDKSITKIEAALLNVLEQTPP
                     ELYADIVNRGIYMVGGGALLRGFDRRLADKININFNVVEDPLHSVARGTGVALKNVEK
                     YPFLMR"
     gene            4510..5331
                     /gene="mreC"
                     /locus_tag="PLZ75_02055"
     CDS             4510..5331
                     /gene="mreC"
                     /locus_tag="PLZ75_02055"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOS71213.1"
                     /translation="MRNLLNFFKRYNNVIIFLILEGIALSLLASGNSYHNTRLIKGIR
                     GITAGIEKSFSRTASYFRLRETEMNLARENAMLRNLLEQAGKMRDNSFSTVTDSVYRQ
                     KYLWFSAEVINNSVNRRKNFLTLDKGYNDDLAPDMAVIADDAVAGVIVGCSSNYSVAM
                     SLLNTDFRLSARIRSNGYFGSLSWDGKSYRHAVLNDIPQHVTVVAGDTVETTGYSAIF
                     PEGLMIGVIDEIERSGSDFYRISVALKADFKKLRFVNLAGNLQKTEQQNLETQFQ"
     gene            5328..5834
                     /locus_tag="PLZ75_02060"
     CDS             5328..5834
                     /locus_tag="PLZ75_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOS71214.1"
                     /translation="MINRILRYTLIFVLLIFLQLFLFNNIQFSGYVNPYVYIMFIMLL
                     PFDISSWLLLFLSFLTGFVIDVFSGTPGMHTSATVAAGFVRPWVLRLISTRGDYEFVS
                     EPSLRIYGFKWFLIYTLVIVFIHHFFLFYLEVFRLTDFFTTFLRVLISTLFSVVFIVL
                     LEYIRRGK"
     gene            5845..7689
                     /gene="mrdA"
                     /locus_tag="PLZ75_02065"
     CDS             5845..7689
                     /gene="mrdA"
                     /locus_tag="PLZ75_02065"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOS71215.1"
                     /translation="MKDLYAARKYFIIAMIVIASAVLVVRLFVIQVVKDVYRLSADNN
                     VLRYVTQYPARGLIYDRNGKLIVFNQAAYDLMVIPSQTSAMDTALFCDDLEISIEAFR
                     ERMKAARSYSYRAPSIFLKQISAESYARFQEKIFMFPGFYVQSRTLRKYSKPVAAHIL
                     GYVSEVDEMQIKKDPYYKPGDYIGKLGIEEAYEKELRGTKGVKIFLVDVYSRVKGSYA
                     DGTMDTAAVQGNDIVSSIDLDLQEYGERLMKNKTGSIVAIEPSSGEIIALVSSPCYDP
                     ELLVGRARSENFMKLSADTLLPLFNRAFMASYPPGSTFKPVNGLIALQEKVIVPSTSF
                     SCNRGYLFVGCHTHSSPLDLKGGIMNSCNAYFCQTFRRILENPAYSSVTEAYEKWRSY
                     LTQFGFGTRLETEFVNELPGLIPTPAYYDRYHGKNGWKALTVISLAIGQGEIGATPLQ
                     MANMTAAIANRGYYITPHVVRPAGGEGEAGSRYETRHHIEIDSANFEEVILGMEAAVN
                     GGAGATARIAALPDVTVCGKTGTVQNPHGKDHSVFIAFAPKHDPQIAIAVFVENSGFG
                     ATYAAPVASLMIEKYLKGEISAASKWKEQRMLELDLIGMKNRQRDEER"
     gene            7676..9097
                     /gene="rodA"
                     /locus_tag="PLZ75_02070"
     CDS             7676..9097
                     /gene="rodA"
                     /locus_tag="PLZ75_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOS71216.1"
                     /translation="MRNVNIWKRIDKPVVLVYLLMVIMGWFNIYAAVFNEEHREIFDF
                     SQRYGKQFIWIIAALVLAVLVMVTDTRFYFFFYWVIYGIMILMLILLFFFGKEINGSR
                     SWFEFGFVSLQPSEFAKFATALALAGYLNYHRQELTRFRIILPALGIIILPALLTAAQ
                     PDMGSTIVFFALILVLFREGMSPYVFVSGLLMIFLFFLALLMDNYMLELILTGLAFFL
                     TWLVTRKLKVALAGAGIIVLIAAPLFIVNHYLNEPASYNVIIVITLLVAGLFFAYYIY
                     QKKALGVLVIYAFLIGSVLYVNTVDFVFDNFLKPHQKERVEIMLGFKSDPHGTGYNLN
                     QSLISIGSGGFSGKGFLRGTQTKFKFVPAQSTDFIFCTVGEEWGFLGSVLIVGLYLFL
                     MLRLIWLAERQRSVFSRTYGYGVVSILFIHFFVNLGMTIGLVPVIGIPLPFFSYGGSS
                     LWGFTILLFVFLRLDASRSEHLI"
     gene            complement(9098..9709)
                     /locus_tag="PLZ75_02075"
     CDS             complement(9098..9709)
                     /locus_tag="PLZ75_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HOS71217.1"
                     /translation="MNVLAHIYLSGDSEKIIIGNYIGDYVKGRDYLKYPEQVRKGIIL
                     HRHIDMFTDRHPVVHRSKIHFSRKYHKYAGVVTDIIYDHFLTREWSLFSRRPLESVTY
                     NFYRAMVNNYDILPDKVKEMMPFFIINNWIETYQTRRGIGRTLRTLSRNTTLPNETRF
                     AIRMLKKNYYTLLDDFMEFFPQLIDYVENDFGIEISHRITVPF"
     gene            9954..11291
                     /locus_tag="PLZ75_02080"
     CDS             9954..11291
                     /locus_tag="PLZ75_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOS71218.1"
                     /translation="MKRKNVIIIGAAGRDFHNFNTVYRDNEDYNVVAFTAAQIPDIDG
                     RKYPSGLAGRLYPGGIPVFPEEDLPGLIRKYSVDDCVFSYSDVPYQKIMSTGAVVNAA
                     GANFVMLGPSATMIRSTKPVIAVGAVRTGCGKSQTSRKIIEILMARGLKVVAVRHPMP
                     YGNLIEQKVQRFAVLEDLAKHKCTVEEMEEYEPHIERGNVIYAGVDYEAILREAEKDP
                     DGCDIILWDGGNNDFPFYRPDLMITVADPHRPGHELNYYPGEVTLRMADVVVINKVDS
                     AEPGAVDIVRENTSRVNPGAVVIDAASPIRVDDPSLIRGRKVLVVEDGPTLTHGEMKI
                     GAGIVAARKFGAAETIDPRPFLSGKLKDTFSQYPGIGSLLPAMGYGEQQLRDLEATIN
                     NTVCDSVVIATPIDLKRIIDIRKPATRVYYELQEIGDPTLEQVLDDFLAKSGR"
     gene            complement(11346..12425)
                     /locus_tag="PLZ75_02085"
     CDS             complement(11346..12425)
                     /locus_tag="PLZ75_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOS71219.1"
                     /translation="MRIELRKDFKWSLVVPTSMGVRITPLHGQPVHCSDTFHMHATSA
                     ESNVASVSSYLGLPVKVLTTFVKDSPVARFIKDNLASRHMAYEGIEVDQGGPWGYRHQ
                     FNIADSGYGTRGPRVQNDRAGEVGRTLNVKDFNLARLFGEEGVQILHLSGLIAALSPE
                     TSNFCLELARAAKKHGTRISFDLNYRASFWKNREEELSATFREIAGIADILVGNEEDF
                     QLCLGVEGPEEGGKDLAAQIESFKEMIRRVRKAFPGVSVFGNTLRQVISVNEHLWGAI
                     VHETDNWHVIEPRRINVLDRIGGGDGFVGGLLYSILKGWEPGKWIQFAWASGALATTM
                     LTDYAQPADEEQIWSIWEGNARVRR"
     gene            complement(12506..13849)
                     /locus_tag="PLZ75_02090"
     CDS             complement(12506..13849)
                     /locus_tag="PLZ75_02090"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOS71220.1"
                     /translation="MEKHISIDLKNSADFISWEEVIALALQSVRHLDALNSGTGAGND
                     FLGWLSLPEDIQVQLDEIKKRARHLRSVSDTTVVIGIGGSYLGSRAVTEALSHSFAPL
                     LKSSQHQMLFAGQNICEDYISDLTDILDDRNYSVIVISKSGTTTEPAIAFRIIKDHLE
                     RKVGKAKAADRIIAVTDSSRGALRSLAELNGYETFVIPDDVGGRYSVLSPVGLLPLAV
                     AGMDIRKLVKGARDMELITRKNRNAENNPALLYAAARNLLLQTGRSVEVLVGFTPKLR
                     YFSEWWKQLYGESEGKDGKGIFPASVEFTTDLHSLGQYIQEGQRILFETILLVNETEK
                     KVTIPSEKDDSDQLNYLACKRLSEVNRSAGEGTILAHNDGNVPVMRINIPLLNEYYVG
                     QLIYFFEIACALSGYILDVNPFDQPGVEAYKKNMFALLNKPGFEEEGKKLRARLK"
     gene            complement(13959..14687)
                     /locus_tag="PLZ75_02095"
     CDS             complement(13959..14687)
                     /locus_tag="PLZ75_02095"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOS71221.1"
                     /translation="MKIMKNLKNLLPLILVLVISSGAHAQFRRTVYGNGRVITSERTP
                     VNASGIKVSTGIDIYLRQGDEPELAVEADENLHGHIIARERNGILEIYTDVNIRRAKM
                     KTVYVTLKEIKSIATSSAGNITGESAIESDNLHIVASSAGDIKLEVYARKIDAGISSS
                     GDVTLSGEADMLSAVLSSAGNLNAYNLRVKEADVRASSAGDAGICVSERLTARASSAG
                     DIYYKGNPLYIDTRASSAGSIRRR"
     gene            15031..15924
                     /locus_tag="PLZ75_02100"
     CDS             15031..15924
                     /locus_tag="PLZ75_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS71222.1"
                     /translation="MSVYSAEKNVSGSFTISGAYALTAILKNWAQDFMSIHQDVKIEI
                     LEKGTGQGIVDLIDGKADLAMISRPLTDEEKEAGIWMMPVAKDGVAIIVNDNNPYLPR
                     LLKQGLSPDEIQKLFTAVPSPAWGELLDTTGSARAVVYTRADESGAAELLAGFCFRKP
                     IDLKGTEVTGDEEMITRVRNNIFSAGFCNLSYAFNKVTGERIENIQIVPFDLDYDNKI
                     GKIEKPFSDIETAHRSVWLGIYPESLCRDLTLGSVGKPDDPAILAFLDYVIHDGQKIV
                     KEAGLCELNNVALRFARESLK"
     gene            complement(15946..17538)
                     /locus_tag="PLZ75_02105"
     CDS             complement(15946..17538)
                     /locus_tag="PLZ75_02105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS71223.1"
                     /translation="MKRIYGLMFSCTFVLSCLVLTGSGSPDYSGTGRDLSGTESARNN
                     QPVKINPFGLEIIPPSSGVQFYRNSIVFLGFSKNFQKMIPSHVSFGTTQAYYAIPGDS
                     TLTDPRVFSADFMFSFPCDAISFSSDYQRMYFTAREEGGEHYKIYSSDYSVKRKGKGS
                     WSKPGRPLDFCNGEFSCTHPALSHDGQMMVFASDRDGTAGKYDLYISRKNGEKWSAPE
                     NMGNLVNTGGDELYPFLDNQNNLYFSSDGHPGYGGLDIFVSKYNGKNWEEPVNLTGDL
                     NTPGDDIAFTLNRQDGKTGFYTSTSGSGKKETRLCKISTGNDNASERLADLAEILIRN
                     AEADTTFTFRKLLLAQADDIARAEMEARAAEAERAVRMEKGEKAEKALKAAKEEEARA
                     AEAKRLAEEARAAEAKRLAEEARAAEAKRLAEEARAAEAKRLAEQAKAAGASEVPANL
                     KDVVVYRVQFLSGPAGKEIKELIVDGKSYTPFVYYYMKEYRYTVGEFTALEPARQFQS
                     AVRKAGYPQAFVATFKNNERIR"
     gene            complement(17595..18527)
                     /locus_tag="PLZ75_02110"
     CDS             complement(17595..18527)
                     /locus_tag="PLZ75_02110"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOS71224.1"
                     /translation="MSGAKKVLFLILLLPSVMASGQGAPFNPVSGRIFTQYVFNPAIA
                     GSRDFLSVDLLASLQDKQHSQLLGAHMRIGKKEHGSRPESGIREFSNIGAGAYIWNDN
                     TGSGRNAGAALAGAYHIPLGRERHSFLSLGAAAKGFFSHSPGDADLNIAARDTVFPDL
                     DAGIYFYNPVFHIGLSATNIFSRNPVNGTDGTYEKYVLNTGFKILISRSLRIILEPLM
                     IAGMGDALPDKIRDVFHPGMKLYLGNFCMGSYLNDYERIPFFFQYKYPGFYIGTFFDL
                     PRNTPFYKKDITAEIALGINIIKNKTAYNRIDQW"
     gene            18674..19045
                     /locus_tag="PLZ75_02115"
     CDS             18674..19045
                     /locus_tag="PLZ75_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS71225.1"
                     /translation="MAESAAPGPAIEGYSNFGYFRLAEKYPVKLIDLDQEKHFRPHPV
                     RFSSILLSPDSYLISAARMKTHDRVVVTLSLKNIIFGAPVKDPGFSWGKTRREGAWSD
                     KAIVHGSGFRGTNYNLFDLAV"
     gene            19244..>19965
                     /locus_tag="PLZ75_02120"
     CDS             19244..>19965
                     /locus_tag="PLZ75_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71226.1"
                     /translation="MSESILNALVQLFALIADIHGETVITGRGKDVVRAFLSRHLNNE
                     LTTRYMGKFEEYLMVYNAEDIERDSIRDRKRISLNAVKILTICQKINEELQQKQKIYV
                     LIQLMDFISLGEKVTENELDFLATVADSFFVPKDEYSDIRSFVIDDARGPDRNSRTVV
                     INNRKEQEGRGDRHLYSEHLEGNLSFLFIASTNTYIFRYSGSEDLYLNGQNIIPDQTY
                     IFDHGSSIRGSGIRTIYYTDIS"
BASE COUNT         5262 a         4512 c         4789 g         5402 t
ORIGIN      
        1 gtgcattaca ggtaattttc cctacaatat ctcatcccag atccttttca gggtactgga
       61 atacaggaat aaagtaacgg aagtgtgtgg tatgtttcag aaggaagtgg ccgaaaggat
      121 atgttcaccg cccggatcac gggtctatgg aattctgagc gtgcttctcc aggcatatta
      181 ccataccgag tatcttttca cggtacccgg tgaagtattt tctcccccgc cgaaagtgaa
      241 ttccggagtg gtcagactgg tcaggaacga tgtgaaggaa ctggaagtga atgagactct
      301 gttcagacag gtagtaaaag ccgccttcag ccagcggcgg aaaaagctca ggaactccat
      361 caggtcagct tttgcacttc gcagcgaaga ttgcccgctc tttgacctga gaccggaaca
      421 gttgtcagtt aacatgtttg ccgatctgac gaaatggatt gaagaaaacc gggtggttta
      481 gccggacagg atctgagttc agctgaatga atatccgatc gcatcaacag gattcatgcg
      541 ggctgcggaa taggcagggg catagcccgc aataatgccg ataactccgg aaatgacgat
      601 ggcaagcatt atgtttgaga atgtaaggtg catgttcagc tcataaagat agtttaccgc
      661 tattgtaccg ataaagatca ggaatatgcc gaatatcccc ccgatcaccg aaagcagaac
      721 cgattcggca agaaactgct ggaggatgaa ggagcgtttt gcgccaaggg ccttctgaat
      781 gcctatcatg tttgttcttt cctttaccga gacgaacatt atgttggcaa tcccgaatcc
      841 tcccacaagg attgaaaaac ctcctatcag ccagcctcct atatttattc ccgtgaacac
      901 cgggtcgaat ccctttgaaa gcattgtagg ggtgtttacc gaaaaattct ccggctcaac
      961 cggcttcaga cgcctggctg atctgagtat catcgttgcc tcatccgaga gctcctgcac
     1021 attaacgccg ggttttgcct tgactatcag ctgggtgttt acaaatctgt tcctgaggtt
     1081 gatgaagttc ttgccgaaat taaggggaat gatggtgatc tgatcaatat tgctgagatt
     1141 tattccgcct tccccttcct ttttcagaat cccgataacc cttacctttc gtccctgaag
     1201 ctttatttcc ttccctaaag gattttcatc cttgaacagc ctttcggccg tttccgctcc
     1261 cagcactgcc acattggtac cgctcgtata ttcatagggg gaaaaatacc ttcccttctc
     1321 aatttcgaat gataatacct tttccaggtt atccgttact gctgcgaccg ctgccccgcc
     1381 tgcggaatta ttcctgtagc tcaggcttgc ggaatggccc actaccagag aaagagactc
     1441 agtccgggtg gatctgcctg ctattgcatg gtagtcatcc agcgcgactg ccggccaccg
     1501 gattatgtcc caccatttga gactggctcc cattgaccag ggatacttct gaatgtacac
     1561 aacattgctt cccattgaac tgatgctgtc gcgtatggct ttctccatcc agtcgagcac
     1621 tgtaaagacg gatatgatgg aaaaaatccc tatggtgatc ccggaaaggg tgagaaatgt
     1681 ccttaattta ttgatcatca cagaacccag tgcaagaatc acactttcct tcagcagctt
     1741 cagatacttc atcccctgtt tgaatattgt tacggaatcc aaagataaac aaataaaaca
     1801 gcaggtaatt tgaaaaagcg gaaaagtatg ttcattttat gctgataatc attattattt
     1861 aacatattga ggtttgtaaa aaaataccta tttttactcg cccgctgaat ctatttccgg
     1921 caatcgtaat aatcagacaa tgagtgaaag aaaaatcaaa agcgccttaa tttccgtata
     1981 tcacaaggaa ggactggatg atatagtaag tctgcttaac cagctgaaag tgagaatatt
     2041 ctcaacggga ggcacctggg atcatataac ccgcacgggt ataagggcag aaaaggttga
     2101 agatcttacc acttatccct ccattctcgg cggaagggta aaaaccctgc acccgtctgt
     2161 tttcggggga atacttgcaa ggagggaaaa cagcggtgat atggagcaaa tggacagata
     2221 cggcattccg gagattgacc tggtgatcgt ggatctttat cctttcgggg aaacagtcag
     2281 gaatacggat gatgaggcag agataacgga aaaaattgat atcgggggca tttcgcttat
     2341 cagggctgcg gcaaagaatt tcagggacgt ggtggttatt tcttccaggg atcagtacag
     2401 ccgcctgctg gagcttctca ggggaaaggg aggtatgacc tccctacagg agagaaggcg
     2461 ctttgccgcc gaggcattca tgaactcatc acattacgac actgaaatat tcaggtatta
     2521 caatctcagg gaaaagctcc ccgtattcag ggagagtata aatatttccg gagagctcag
     2581 atacggcgaa aatcctcatc agaaaggaat ttactacggg gatccgggtg agatgtttga
     2641 aaagctgcac gggaaggatc tctcctacaa caatctgctg gatatagaat ctgcggtcac
     2701 tcttattgac gaattcacgg aaacgacagt gatcatcatc aagcataaca atgcctgcgg
     2761 ggcagcttca agggataatc tcaccgatgc atggaatgat gccctggcat gcgatcccgt
     2821 atcggcattc gggggaataa ttgctgccaa cagggaaatt gatgagcaga cagcttctgc
     2881 aatggacaag atattctttg aggttctgat agcacccggc ttcagtgcaa aagccctgga
     2941 aatactggaa cagaagaaaa acaggatcat tttaaaaaga aaagctgttt tcaggatgga
     3001 ggctgctttc agatcacttc tgaacggtgt cctgtggcag gaaagggata acagtacaca
     3061 gacccctgag gatctgatgc ccgtaactcc ccggaaacct gataagaatg aaacagaaga
     3121 tcttgtattt gccaatatcc ttgtaaaaca cagcaagtca aatgcgattg tcctggcaaa
     3181 aaacaaacag ctgtgtgcaa gcggaatagg acagacatca agggtggatg cactgaaaca
     3241 ggcaattgag aaagcccgtt tgttcggttt tgacctgaaa gattccgtta tggcatcaga
     3301 tgccttcttt ccctttcctg actgtgtgga gattgctcac aaggcagggg ttacagcggt
     3361 ggttcagccg ggaggatcgg tcagggataa ggattcaata gatttttgtg ccgccaacgg
     3421 aatggcaatg gtatttacag gaataagaca ttttaaacat tgataattga tatatgggac
     3481 tattttcatt tttgacacag gagatcgcta ttgacctggg tacagccaat actattatta
     3541 ttcagaatga caagattgta gtggatgagc cttcgatagt tgctattgac aagaatacgg
     3601 gaaaacttat agcaataggg gaggctgcca ggcagatgca tggcaagaca catgaaaata
     3661 tcaagacaat aaggcctttg cgtgacgggg tgattgccga tttcaatgca gcagaactga
     3721 tgataagggg aatgataaag atgatcaaca agggaccgaa acttttttcc ccgtcaatga
     3781 aaatggtcat ttgcatacct tccggaagca ctgaggtgga aataagggct gtgcgcgact
     3841 catcagagca tgccggggga agggatgttt acattattta tgagccaatg gcggcagcca
     3901 tcggtatcgg tatagatgtt gaagcacccg agggttcaat gatagtcgat ataggcggcg
     3961 gtaccacgga gattgcagtt attgccctgg gagggatagt atgcaataag tcaatcagga
     4021 ttgcaggaga cggattcact gcggatattc aggcatatat gaggcatcag cataatataa
     4081 aaatagggga aaggacggcg gaagatataa agatagctgt cggtgccgca tttcccgaca
     4141 tagataatcc gcctccggac tacatagtga gggggccgaa cataatgact gcccttccga
     4201 ttgaaatacc tgtatcctat caggaaatag cctactgcct tgacaagtcc attaccaaga
     4261 ttgaggcagc ccttctcaat gtgctggaac agaccccgcc ggaactctat gccgacattg
     4321 tcaacagggg aatatacatg gtgggaggcg gtgccctgct caggggattt gacagaaggc
     4381 tggccgacaa gattaatata aacttcaatg tggttgagga tcctcttcac tcggtggcaa
     4441 gaggcacggg cgtggcactg aagaatgttg aaaaatatcc tttcctcatg agataatatc
     4501 cggccgtgaa tgagaaatct ccttaatttt tttaagagat acaataatgt gataatcttc
     4561 ctgatacttg aaggaatagc attgtccctg ctggcatcgg gcaacagcta tcacaataca
     4621 agactgataa agggaatcag gggaattacg gccggtattg aaaaatcatt cagcagaacg
     4681 gcttcctatt tccgcctgcg tgaaacggaa atgaacctgg cacgggagaa tgccatgctg
     4741 aggaatcttc ttgagcaggc gggaaaaatg agggacaaca gcttttccac tgtcactgac
     4801 tctgtttaca gacagaaata tttatggttc tctgcggaag taataaataa ttcggtgaac
     4861 cgccggaaaa acttcctgac cctcgacaag ggatataatg atgatctggc acctgatatg
     4921 gccgttattg cagatgatgc cgttgccgga gtgatagtgg gatgctcgtc aaactattct
     4981 gtggcaatgt cccttctcaa cactgatttc agacttagtg caaggataag gtcaaacgga
     5041 tatttcggtt cactgagctg ggacgggaaa agctacaggc atgccgttct gaatgacatt
     5101 ccccagcatg tgacggttgt tgcgggtgat actgttgaaa caaccggcta ttctgccatt
     5161 ttccccgaag gacttatgat tggcgtgata gatgaaatag agagatcggg aagcgatttc
     5221 tacaggatct cggtggccct gaaggctgat tttaaaaaac tcagatttgt caacctggcc
     5281 ggaaaccttc agaagacaga acagcagaat cttgaaacac aattccaatg atcaacagga
     5341 tactcagata caccctgata tttgtcttgc tgatatttct tcagcttttc ctgttcaaca
     5401 atatacagtt cagcgggtat gtgaatccct atgtctatat catgttcatc atgctgctgc
     5461 cctttgacat atcgtcatgg ctgcttcttt ttctttcctt tctgacggga tttgttattg
     5521 atgtcttctc cggtaccccg ggaatgcaca cttccgcaac tgtagccgcc ggattcgtgc
     5581 gcccctgggt gctgagactg atttccacca gaggggatta cgaatttgtg tcagaaccat
     5641 cactgaggat ttacggattc aaatggtttc tgatatatac ccttgtgatc gtttttatcc
     5701 atcatttctt tcttttttac cttgaagtgt tccggctcac tgattttttc actacatttc
     5761 tgagagttct gataagtaca cttttttccg tggtttttat agttcttctt gaatatatca
     5821 gaagagggaa atagtcagcc gtttatgaag gatttgtatg cagccaggaa atatttcatt
     5881 atcgcaatga tagtcattgc gtctgccgtg cttgtcgtaa gactgttcgt catacaggta
     5941 gtaaaggatg tatacaggct ttcagctgat aataatgttc tcagatatgt aacccagtat
     6001 cctgccaggg gactgatcta tgacaggaac gggaaactta ttgtttttaa ccaggcagcg
     6061 tatgacctca tggtgattcc gtcacagaca tccgcaatgg ataccgcact tttctgcgat
     6121 gatctggaaa taagcatcga ggcattcagg gagagaatga aggctgccag aagttattcc
     6181 tacagggctc cttcaatatt cctcaagcag atttcagctg aatcctacgc aagattccag
     6241 gaaaagatat tcatgtttcc gggattctac gtacagtcaa ggacactgag gaaatattcg
     6301 aagcctgttg ctgcacatat actgggctat gtcagcgagg ttgacgagat gcagataaag
     6361 aaggacccgt attacaagcc gggcgattat atcggcaagc ttggcattga agaggcctat
     6421 gaaaaggaat tgagaggcac gaagggcgtg aaaatattcc tggttgatgt ctacagccgt
     6481 gtaaagggat cctatgccga cgggaccatg gacacggcag ctgttcaggg aaatgatatc
     6541 gtttccagta ttgatcttga tcttcaggaa tacggtgaac ggctgatgaa aaacaagaca
     6601 ggaagcattg ttgccatcga gccgtcatcg ggtgagataa ttgccctggt ttcgtcaccg
     6661 tgctatgatc cggagcttct cgtaggaagg gcccggtcag aaaacttcat gaagctgtcg
     6721 gcagacaccc tgctgccatt gttcaacagg gctttcatgg cctcatatcc cccgggatca
     6781 accttcaagc cggtcaacgg cctcattgca cttcaggaaa aggttatcgt gccatcaaca
     6841 agcttctcct gcaacagggg ctatctgttt gtaggctgcc atacacattc atcccccctt
     6901 gacctcaagg gaggaataat gaattcgtgc aatgcatatt tctgccagac attcagaagg
     6961 atacttgaaa atcccgcata ttcatccgtt acggaggcat atgaaaaatg gaggtcatac
     7021 cttacccagt tcggtttcgg aacaaggctg gaaaccgaat ttgtgaatga gctgccgggc
     7081 ctgattccta ctccggcata ttatgacagg tatcacggaa agaacggatg gaaggcactt
     7141 acggtcattt ccctcgcaat agggcagggc gagataggag ccactcccct tcagatggca
     7201 aacatgactg cagcaatagc caacagggga tattatatta ctcctcatgt tgtaaggcct
     7261 gccggcgggg aaggggaagc aggcagccgc tacgaaacaa ggcatcatat agaaattgat
     7321 tctgccaatt tcgaggaggt tatcctcgga atggaggcag ctgtgaacgg aggagcgggg
     7381 gcaacagcaa ggattgccgc actgcccgat gttaccgtat gcgggaagac aggcacggta
     7441 cagaacccgc atggaaagga ccattccgtt tttattgcat tcgcaccgaa gcatgatcct
     7501 cagatagcca ttgcagtgtt tgttgagaat tcaggttttg gagccaccta tgcagctccc
     7561 gtggccagcc tgatgataga aaaatatctt aaaggggaaa tatcagcggc atcaaaatgg
     7621 aaggaacaga ggatgcttga actggatctt atcggcatga aaaacaggca gcgtgatgag
     7681 gaacgttaac atatggaagc ggatagacaa gcccgtagta cttgtctacc tcctcatggt
     7741 catcatggga tggttcaata tttatgctgc cgtgttcaac gaagagcaca gggagatatt
     7801 tgacttttcg caacgttacg ggaaacagtt catctggatc atcgcagccc tggtactggc
     7861 cgtattggtt atggttaccg atacaaggtt ttactttttc ttttactggg ttatttacgg
     7921 catcatgatc ctgatgctga tcctcttatt ctttttcggc aaggaaataa acggctccag
     7981 gtcatggttc gaattcggat ttgtaagcct ccagccttcc gaattcgcca agtttgcaac
     8041 ggccctggcc ctggccggct atctcaatta ccacaggcag gaactcacca ggttcaggat
     8101 aatactgccc gcactgggga ttatcattct gcctgccctc cttacagctg cccagcccga
     8161 catggggtca accatcgttt tctttgcact gatactggtg ctgttcaggg aaggaatgag
     8221 cccctatgtc tttgtgtcgg gtcttttgat gatctttctt ttcttccttg ccctcctgat
     8281 ggataattac atgcttgaac tgatactgac cggactggca ttttttctga cctggcttgt
     8341 tacaagaaaa ctgaaagtgg ctcttgccgg tgcgggcatc attgtactta ttgccgcacc
     8401 cctgttcata gtgaatcatt atctgaatga accggcttca tataatgtga ttattgtaat
     8461 aacactgctg gtggcgggcc tgttttttgc ctattatatt taccagaaaa aagccctcgg
     8521 agtacttgtt atttatgctt ttctgattgg cagtgttctg tacgtcaata ctgttgattt
     8581 tgtgtttgac aattttctga aaccgcatca gaaggaaagg gtggaaatca tgctcggatt
     8641 caaatcagat ccccacggaa cgggttataa tctgaaccag tcacttattt ccatcgggtc
     8701 gggaggtttc agcggcaagg gtttcctgcg ggggacccag acaaaattca agtttgttcc
     8761 ggcacaaagt accgacttca ttttttgcac ggtcggagag gaatggggat tcttaggttc
     8821 agtccttatt gtgggccttt atctttttct catgctgagg ctgatctggc tggccgagcg
     8881 gcagcgttcg gtattttcgc gaacttacgg atacggagtg gtatcaattc tgttcattca
     8941 ttttttcgtg aaccttggaa tgacaatcgg acttgttccg gtgataggaa tacctcttcc
     9001 ctttttcagt tacgggggaa gttcactatg gggtttcacc atcctgctct ttgtatttct
     9061 gaggcttgat gccagccgtt cggagcatct gatatgatca gaacggaaca gtgatcctgt
     9121 gagagatctc tatcccgaaa tcattttcca cgtaatcgat cagctgcgga aagaattcca
     9181 tgaaatcatc cagcagagtg taataattct tcttgagcat gcgtattgca aaccttgtct
     9241 cgttgggcaa tgtggtgttc ctgctcagtg tcctcagtgt ccgaccgata cccctgcggg
     9301 tctgataagt ctcaatccag ttgttgataa tgaaaaaggg catcatttcc tttaccttgt
     9361 cgggaaggat atcatagtta ttgaccattg ccctgtaaaa attataggta acactctcca
     9421 ggggcctccg tgaaaacagg ctccactccc gtgtaaggaa atgatcatag atgatatctg
     9481 tgacaacacc ggcatattta tggtatttcc tcgagaaatg tatcttgctt ctgtgaacca
     9541 cgggatgcct gtcagtaaac atatcaatat gcctgtgcag gattatgccc ttccgtacct
     9601 gttcgggata tttcagataa tccctgccct tcacataatc acctatgtaa tttccgatga
     9661 ttattttttc ggaatcaccc gacaggtata tgtgggccag gacattcatt tatgcttata
     9721 atagtacaaa ttatttacga attttttcat taaatggttc actttgcaaa ggccctatca
     9781 ggattcaacc tcttattata agatatattg tatcacagac agttaaaatc tgaaagtcat
     9841 ttaaaaaaaa tgacttaaaa tttcgttaat ccgccatgca aattatacat ttgctcattc
     9901 attttaaaat gaaaaacagc ctgtcaataa attgatgata aaaattatta aaaatgaaaa
     9961 gaaaaaatgt aatcataatc ggtgctgcag gaagggactt ccataatttc aataccgttt
    10021 accgcgacaa tgaggattat aatgttgtgg cattcacagc tgcccagata cctgatattg
    10081 acggcaggaa atatccttcc ggactagcgg ggaggcttta tcccggcgga atacccgttt
    10141 ttccggagga ggatcttccc gggcttatca ggaagtacag cgttgatgac tgtgttttct
    10201 cctacagtga tgtgccctat cagaagataa tgtcaacagg tgcggttgta aatgcggcag
    10261 gagcgaattt cgtcatgctg ggtccttcgg cgacgatgat cagaagtacc aaaccggtca
    10321 ttgccgttgg tgcggtcagg accggctgcg gcaagagcca gacatcaagg aagataatag
    10381 agattctgat ggcaagggga ctgaaggttg ttgcggtccg gcatccaatg ccctatggca
    10441 acctgattga gcagaaggtt cagcgctttg cagtccttga ggatctggca aaacataaat
    10501 gcaccgttga ggagatggag gaatatgaac cccatattga aaggggcaat gtcatatatg
    10561 ccggtgtgga ttatgaggct atactccgtg aggcagagaa ggatcctgat ggctgcgaca
    10621 tcatcctgtg ggacgggggc aataatgatt tcccctttta ccggcccgat cttatgataa
    10681 ctgttgccga tccccacagg ccgggccatg aactgaatta ctatcccggt gaagtgaccc
    10741 tgcgtatggc tgatgttgtt gtaataaaca aggttgacag tgctgagccc ggagcagtgg
    10801 atattgtaag ggaaaataca tccagggtca atccgggagc tgtggtcatt gatgccgcat
    10861 caccgatcag ggttgatgat ccttccctga taaggggcag gaaggtgctt gttgtggagg
    10921 acggtccgac ccttacacac ggggaaatga aaataggagc cggaatagtt gccgcacgga
    10981 aattcggtgc tgctgaaaca atcgatccca gacctttcct gagcggcaaa ctgaaggaca
    11041 ccttcagtca gtatccgggc attggcagtc ttcttcctgc aatgggatac ggggagcagc
    11101 agctgcgcga ccttgaggcg acaataaaca ataccgtctg cgacagtgtt gtgatagcaa
    11161 cgccgataga ccttaaaagg ataattgata tcaggaagcc ggcaacgagg gtgtactatg
    11221 agttacagga aataggggat cccacgctgg aacaggtact tgatgatttc ctggcaaaat
    11281 ccggccgctg aaaaacagac ggtcccgtaa tgcaggcggg accgtccctg tgaatatcta
    11341 tattttcagc gtcttacacg ggcgttgcct tcccagatgc tccagatctg ttcttcatcc
    11401 gcaggctgtg catagtcggt aagcatggtt gtggcaagtg caccggaagc ccatgcaaac
    11461 tgaatccatt ttccgggctc ccagcctttg aggattgaat aaagcagacc gcccacaaat
    11521 ccgtcaccgc cgccaatcct gtcaaggaca tttatccggc gcggctcaat cacgtgccag
    11581 ttgtcagttt catgcactat tgcaccccac agatgctcat tgacgctgat gacctgacgg
    11641 agggtgttgc caaaaacgga aacaccggga aacgcttttc tgactcttct tatcatttcc
    11701 ttgaaacttt caatctgagc tgcaagatcc tttccgcctt cctccggtcc ctcaacaccc
    11761 aggcagagct ggaaatcctc ttcgtttcca accagtatat cggcaattcc cgcgatttcc
    11821 ctgaaagttg cggataattc ctcttcacgg ttcttccaga aggatgcccg gtaattcaga
    11881 tcgaaagata tcctggtgcc gtgttttttt gcagcacggg ccagttcgag gcaaaaattg
    11941 ctggtttcag gcgacagggc ggcaataagt cctgaaagat gcagtatctg aaccccttct
    12001 tcaccgaaga gcctggccag gttgaaatcc ttgacattca gtgtccggcc cacctccccg
    12061 gcacggtcat tctgcaccct cggccccctg gtgccgtaac cgctgtcggc tatattgaac
    12121 tggtgccggt aaccccaggg ccccccctgg tcaacttcaa taccttcgta ggccatatgc
    12181 ctgctggcaa gattgtcctt gatgaacctg gctaccgggc tgtcttttac aaatgtggta
    12241 agaaccttga caggcaggcc aagataggat gaaacacttg caacattcga ctcggcactg
    12301 gttgcgtgca tgtgaaaggt atcactgcag tggacgggct ggccgtgaag aggagtgatt
    12361 ctgaccccca tgctcgtagg caccaccagt gaccatttga aatcttttct tagctcaatt
    12421 ctcataatgt aaatggttta agttatttat tgattgatta ttctgcaaca ggacagccgg
    12481 ttccggctcc ggtttccccg gctgcttatt tcagtctggc cctgagtttc ttcccttctt
    12541 cttcaaagcc gggcttgttc aggagggcga acatgttttt cttgtaggct tcaacgccgg
    12601 gctgatcaaa gggattcaca tcaaggatat agccgcttaa ggcacaggct atctcgaaga
    12661 aataaatcaa ctggccgaca tagtattcat tcagcagagg gatgttgatc ctcataaccg
    12721 gaacgttgcc gtcattatgg gcaaggatgg ttccctcccc ggcactgcgg ttcacttcag
    12781 agagtctttt gcaggcaaga taattgagct ggtcggaatc atccttctca gatggtattg
    12841 ttactttctt ttctgtttcg tttaccagaa ggattgtttc gaagagaata cgctgccctt
    12901 cctggatata ttgtcccagt gaatgcaggt ctgttgtaaa ctcgactgat gccgggaaaa
    12961 taccctttcc gtcctttcct tcactttcac cgtaaagctg cttccaccat tccgagaaat
    13021 agcggagttt tggcgtaaac ccgacaagta cctcaacgga tcttccggtc tgaagcaaca
    13081 ggttccgggc tgcagcatag agaagagccg gattgttttc agcattcctg ttctttcttg
    13141 ttataagctc catgtcgcgg gctcccttaa ccagctttct gatatccatg cctgctaccg
    13201 ctaatggcag cagaccgacc ggactgagga cggaatacct tccccccaca tcatcgggga
    13261 tcacaaatgt ttcatagcca ttaagctctg ccagcgatct cagtgcaccc ctgctgctgt
    13321 cggtgacagc aatgatcctg tcggctgcct tcgcttttcc gacttttctt tcaagatgat
    13381 ccttgatgat cctgaatgca atagcaggtt cggttgtggt tcctgacttg gaaatgacaa
    13441 tgactgaata attcctgtca tccaggatat cggtcaggtc agaaatataa tcctcgcaga
    13501 tattctgccc tgcaaacagc atctggtgct ggctggattt cagcagtgga gcgaaggaat
    13561 gagagagagc ttcagtcact gccctggaac caaggtaaga gccgccgata ccgataacca
    13621 cggtggtatc tgaaacagat ctgagatgtc tggccctctt cttaatttcg tcaagctgca
    13681 cctgtatgtc ttcgggaaga gacagccatc ccagaaaatc atttccggca cccgttccgc
    13741 tgttgagggc atcaagatgc ctcacagact gtaaggcaag agctatcacc tcctcccagg
    13801 agataaagtc tgcagaattc ttaagatcaa tagatatgtg tttttccata atgaaaggtt
    13861 tttgtttgcc tcaggattaa cgcaaagtta aaaaaataac cgccgggaat attccctgac
    13921 ggttattata aaaccggtat tatatatcgg gaaggaagtt atcttcttct aatgcttcct
    13981 gcacttgaag cccgggtgtc aatatacagg ggattaccct tgtagtatat atctcctgca
    14041 ctggaggccc gtgccgtcag tctttcgctg acacatatgc ctgcatcccc ggcactggaa
    14101 gctctgacgt cagcttcctt caccctgaga ttgtatgcat tcaggttgcc tgcactgctc
    14161 agaacggcag aaagcatgtc tgcttcacca ctcagtgtca catcaccgga actgcttatc
    14221 ccggcatcta tcttgcgggc gtatacttcc agtttgatgt cacctgcact gcttgccacg
    14281 atatgaagat tgtcactctc aatcgccgat tccccggtaa tgtttcctgc gctggaagtg
    14341 gcaatacttt ttatctcttt cagggttaca tatacggttt tcattttcgc acgccggata
    14401 tttacatcag tatatatttc aagaattccg ttcctttccc tggcaatgat atggccgtgc
    14461 agattctcat cagcctctac agccagttcc ggttcgtcac cctgcctcag gtaaatgtct
    14521 attccggtgc tgacttttat tcctgatgca ttcaccggag ttctttcact tgtaattact
    14581 cttccgtttc cgtatacagt cctgcgaaat tgtgcatggg caccggatga aataaccagg
    14641 accaggatga ggggcaataa attcttcagg tttttcatga tcttcattaa ttgggattag
    14701 cgttttattc cgtgttaaag acagtgctta ttaacgtttg ctgcatcaaa tgcatacatt
    14761 atttgcataa aatgatgttt ttacaaaaag aactttctga aacaggaact ttctttggag
    14821 attgttttga gtttgcagct ttttaaactc ttatctttta tgggtatttt gcatcctttt
    14881 ctggttactt acatgtaaat cggggattac tgaaaaaata ttttataatt ttatacaaat
    14941 ttttgaacct tgaaaaagat aactgttctg ttgcttgcat ttgcaataat ttttggctgt
    15001 aagccgcgaa aatccggaac cccagggagc atgtccgtct attccgcgga gaagaatgtt
    15061 tccggaagct ttacaataag cggtgcctat gcccttaccg ctattctgaa gaactgggcg
    15121 caggatttca tgagcattca tcaggatgta aaaattgaga ttctggagaa aggtaccgga
    15181 cagggtatag tggatctcat tgacggaaaa gctgatctgg caatgatttc gcgtcctctt
    15241 accgacgagg aaaaggaggc gggaatctgg atgatgccgg tggccaagga cggtgttgca
    15301 ataatcgtca atgataataa tccctatctt ccccggctgt tgaaacaggg actaagtccg
    15361 gatgagatac agaaactatt tactgccgta ccgtctccgg cctggggaga actgcttgac
    15421 actacaggaa gtgcccgggc tgttgtctat accagggctg atgaatcggg tgctgctgaa
    15481 cttctggcag gattttgttt cagaaaacct attgatctga agggaacaga ggtcacgggt
    15541 gatgaagaaa tgataacccg tgtgagaaac aatatttttt ctgcgggatt ctgcaacctg
    15601 tcatatgcat tcaataaggt gacaggggaa agaatcgaaa acatacagat agtacccttt
    15661 gatcttgact atgacaataa gataggaaag atagaaaaac ctttcagtga tatagagact
    15721 gcccacagaa gcgtctggct gggaatatat ccggaatccc tgtgccgtga cctgaccctg
    15781 ggttcagtgg gcaaacctga cgatcctgcg atactggcat ttctggacta tgttatccat
    15841 gacgggcaga aaattgtaaa agaagccggt ctctgtgaac ttaacaatgt agcactgagg
    15901 tttgcaaggg aaagccttaa gtaagcctga agcaggccgg cttaatcagc gtatcctttc
    15961 gttattcttg aatgttgcaa caaatgcctg ggggtatccc gcctttctta cggcggactg
    16021 aaactgcctg gcaggctcaa gtgcagtgaa ttcccccacc gtgtagcggt attccttcat
    16081 ataatagtat acaaagggag tatatgactt gccgtccacg ataagctcct ttatctcttt
    16141 ccctgctggt ccggaaagga actgcaccct gtaaaccacg acatccttca gattagcagg
    16201 tacttctgat gccccggcag ctttagcctg ttcagcaagc ctttttgctt cggcggccct
    16261 tgcttcctct gcaagccttt ttgcctcggc ggccctggct tcctctgcaa gcctttttgc
    16321 ttcggcggcc cttgcttcct ctgcaagcct ttttgcctcg gcggccctgg cttcctcttc
    16381 cttcgccgct ttaagtgctt tctcagcttt ctcccccttt tccattctta cagctctttc
    16441 agcttccgct gcccttgctt ccatttctgc ccgggctata tcgtcagcct gtgccaggag
    16501 gagtttccgg aaggtgaaag tggtatctgc ctctgcattt cttatgagta tttctgccag
    16561 gtctgcaagt ctttctgaag cattatcatt tccggtgctt attttgcaga ggcgggtttc
    16621 ctttttcccg gaaccggaag ttgaggtata aaagccggtt tttccgtcct ggcgattaag
    16681 ggtgaaggca atatcatcac cgggggtatt caggtctcct gtcaggttaa ccggttcttc
    16741 ccagtttttc ccgttgtatt tacttacgaa gatgtcaagt cccccgtaac ccggatgccc
    16801 gtctgaagaa aaatagaggt tgttctgatt gtccaggaag ggatagagtt catctccgcc
    16861 ggtattgaca aggtttccca tattttccgg tgctgaccat ttttcaccgt ttttcctgct
    16921 gatgtaaaga tcatatttac cggccgttcc gtccctgtcg gatgcgaaaa ccatcatctg
    16981 accgtcatgg gacagggccg gatgggtaca ggaaaactca ccgttacaga aatcaagggg
    17041 cctgcccggt tttgaccagg aacccttgcc ctttcgtttt acagaataat ccgatgaata
    17101 tattttgtaa tgttcccctc cctcttccct tgcggtgaaa tacatccttt gataatcgct
    17161 gctgaaggaa atggcatcac agggaaagga aaacatgaaa tccgctgaaa agacccgggg
    17221 gtcagtcagt gtggaatctc cgggaattgc atagtatgcc tgggtggttc cgaatgatac
    17281 atgtgacggt atcatcttct ggaaattttt cgaaaacccg agaaacacta tgctgtttct
    17341 gtaaaactgc acacctgaag agggaggtat tatttccagt ccgaatggat ttattttaac
    17401 gggctgatta ttccgggctg attcagttcc ggaaagatcc ctgccggtgc cggaatagtc
    17461 aggtgaaccg gagcctgtga ggaccaggca gctgagaaca aacgtgcagg aaaacattaa
    17521 tccgtaaatt cttttcatac tagctggagt tggggtaaat catgctggct caatggacac
    17581 agtgacatgt ttcctcacca ctggtctatc ctgttatatg ccgttttgtt ttttataatg
    17641 ttaattccca gagctatttc cgcagtgata tcctttttat aaaagggggt attccttggc
    17701 aggtcaaaga atgtgccgat atagaatccc gggtatttgt actgaaaaaa gaaagggatt
    17761 ctttcatagt cattcagata tgatcccata cagaaattcc cgagatacag tttcattccg
    17821 ggatgaaaaa catccctgat tttatcggga agtgcatcac ccattcctgc aatcatcagt
    17881 ggttcaagta ttattctgag acttctgctg atcagtatct tgaatcctgt attgaggaca
    17941 tatttttcat atgtgccgtc agtgccgttc accgggttcc gggaaaagat gttggttgct
    18001 gaaaggccta tatggaatac cgggttataa aaatatattc cggcatccag gtcggggaat
    18061 accgtatccc ttgcagctat attcagatcg gcatcacccg gagaatggct gaaaaaacct
    18121 tttgctgcgg ctcccagtga gagaaagctg tgcctttccc ttcccagtgg aatatgatat
    18181 gctcctgcaa gagccgctcc ggcattgcgg ccgcttccgg tattgtcatt ccagatatag
    18241 gctcctgcac cgatgtttga gaattctctt atgccggatt ccggccggct gccatgttcc
    18301 tttttcccta tcctcatgtg tgccccaagc agttgtgaat gctgtttatc ctggagggac
    18361 gccaggaggt caaccgacag aaaatccctg cttccggcga ttgccggatt aaagacatac
    18421 tgggtaaaaa tcctgccgga taccggatta aagggtgcac cctgccctga ggccatgaca
    18481 gagggaagga gcagaatgag aaacaaaacc tttttcgcgc ctgacattga tctttcagaa
    18541 attagacaat ccgaaaatca gaaatacgat tcaaattaca aattgtttca aaatccggat
    18601 attatttatg tgaaatggca gaatttattt tcggatatcc tcccggggaa caagcccctg
    18661 cctcaatgtc ataattgccg aatcagctgc tccgggtccg gctatcgagg gatacagcaa
    18721 tttcggctat ttcaggctgg cagaaaaata tcccgtgaag cttattgacc tggatcagga
    18781 aaagcatttc agacctcatc ccgtcaggtt ctcaagcatc ctcctcagtc cggattccta
    18841 tctgatatcg gcagcaagaa tgaaaacaca cgaccgggtt gttgttactc tttcactgaa
    18901 gaatattatt ttcggggcac cggtaaaaga cccgggattt tcatggggaa aaacccgcag
    18961 ggaaggtgca tggagtgata aggccattgt acacggaagc ggattcaggg gtaccaatta
    19021 caatctcttt gaccttgccg tctgaaaccc tatgacttgt tcacttacgg aaaacgcggt
    19081 actgccgtgg cgctccggat attgacaaca gctgcagtct cctgctcata acttttcttc
    19141 agaaattcgt aaaaatgaat taatttgcac aaaattaatt ctcaggaatt taattccgtg
    19201 ctaattgatt tagcaggact tgtgaaggtc aaaccgaatt taaatgagtg aatccatcct
    19261 gaatgcgctg gttcagttgt ttgcactgat agccgatatc catggtgaaa cggtaattac
    19321 cggcagggga aaggatgttg tacgggcatt cctgtcgcgg catctgaaca atgaactgac
    19381 aacaagatac atggggaagt ttgaggaata cctcatggtt tacaatgcag aggatattga
    19441 aagagacagt atcagggaca gaaaaagaat atccctgaat gccgtcaaga tactcaccat
    19501 ctgccagaag ataaatgaag aacttcagca gaagcagaaa atatacgttc tcatccagct
    19561 gatggatttt atttcccttg gagagaaggt aacggaaaat gaactcgact tccttgcaac
    19621 cgtggcagat tccttctttg ttcccaagga tgaatacagc gatatcagga gttttgtcat
    19681 tgatgatgcg aggggcccgg acaggaactc aaggacagtg gtgataaaca acaggaagga
    19741 acaggaggga aggggagaca gacaccttta cagtgaacat ctggaaggaa acctgtcgtt
    19801 tttattcatt gccagtacca atacctatat tttcagatac agcggcagtg aggaccttta
    19861 cctcaacggc cagaatatta ttcctgacca gacatatatt tttgaccacg gatcatcaat
    19921 aaggggatca gggatccgga ccatttatta taccgatata agcag
//
LOCUS       DAOSPX010000021        19616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_2938_length_19616_cov_3.966259, whole genome shotgun sequence.
ACCESSION   DAOSPX010000021 DAOSPX010000000
VERSION     DAOSPX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2938_length_19616_cov_3.966259"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..263)
                     /locus_tag="PLZ75_02125"
     tRNA            complement(190..263)
                     /locus_tag="PLZ75_02125"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Ile,seq:gat)
     gene            complement(588..974)
                     /locus_tag="PLZ75_02130"
     CDS             complement(588..974)
                     /locus_tag="PLZ75_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOS71227.1"
                     /translation="MIDRFVILKFLKFSVVGFSGMLIDFGVTWLLKEKARANKYIANS
                     TGFILAATSNYIWNRIWTFGSKSEEIAIEYFSFILISVAGLGINNLVIYLLHDRLGMN
                     FYLSKLIAIGIVTVWNFTMNLIFTFR"
     gene            complement(967..2685)
                     /locus_tag="PLZ75_02135"
     CDS             complement(967..2685)
                     /locus_tag="PLZ75_02135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOS71228.1"
                     /translation="MRTPENSLKDSVNRKLNYRSIFILFLAIWFLVNLLQAVFMEILS
                     DEAYYGLFGRYLDWGYYDHPPMVALMTRISSVLFSGNLGIRFMSVLLQTGTIILIWLT
                     ADFRTDDRRTVVIFFLVAASISMFSAYGVLAAPDSPLLFFTALAFFAYRKFLAGAGWK
                     EVLLLAISMAGLVYSKYQAVLVIGFLVLSNLKLLKNYRFLAAGVIALLILSPHIYWQL
                     SNDFPSFRYHLVTRSAGFRWNYFLEYLPNQMAVFNPFTLGAVVYVMFRYRPDSLMKRN
                     YYLQIAGFLVFFWMMSFRGHVEPHWTIACSIPMILILTEKCSSDPSLLRYAGRFILPS
                     ILLLVAIRLILMTDIRFVRDIAFGGKEIKYRELADEAEGLPLVFTGAFQRPSLYNFFT
                     GGEAVAVSSVYSRQTQFDLWQFEKKYHNRPAFICLNQHTNSEIYVSDTMRFGGFRTDS
                     LQTVNRMKIEFDSGEGIRHSGDTLQLSFSLHNPCEFDIDFNHRHFPVSFSMVFIRGKI
                     LYIYDVFPCEPVNIVGPGETVHGMIPAHIPDLPAGKYSFGLSLNTLFGPSLNSSFKKL
                     KIEKDD"
     gene            complement(2682..3716)
                     /locus_tag="PLZ75_02140"
     CDS             complement(2682..3716)
                     /locus_tag="PLZ75_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71229.1"
                     /translation="MKNSLGIILLCLAMPFNAGSQQIGFRTGLYSFFDNTEFGHSRVH
                     IPQTMAGVRFVPGMSVSFDSVHTIVAGVNMLHEYGSTGIIDDLSPVAYYMFRKSGFSF
                     LAGAFPREEALDRFPRLFFRDSISFYRPNMNGILLDYSGRNISANLWLDWTSRQSYES
                     RETFFVGFSGKYNREHFYIRNFSYMFHFAGFMDPVTDEPLHDNLLQLTSAGLDLSGVT
                     FLSRLDFNVGYVTGFDRARALKTGWLLHHGFLSELNIEYRGIGIFNTFYAGDGQMEYY
                     PVHNNELYWGDPFYRTNTYNRTDFYWSFLRSNIIKARLEFSFHFTEGNIYNEQALKVS
                     VNLNNIRMKN"
     gene            3826..4611
                     /locus_tag="PLZ75_02145"
     CDS             3826..4611
                     /locus_tag="PLZ75_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOS71230.1"
                     /translation="MANLIIIPTYLESENIEAITRTIFSLSIPFSILVIDDNSADGTA
                     AIVKNLQTEFKNLHLIERPGKLGLGTAYITGFRWALENDFDYIFEMDADFSHNPDDLI
                     RLYSACHDDGADVAIGSRYISGVNVVNWPLSRVLMSYFASVYVRIITRMNVKDTTAGF
                     ICYRSEVLRNIHLEHIRSKGYGFQIEMKFAAWKLGYKIVEVPIIFTDRKLGTSKMSGG
                     VFNEALWGVIRMKIRSWFMKYSPGGAGMTADAETEKEDDKKKD"
     gene            4781..6124
                     /locus_tag="PLZ75_02150"
     CDS             4781..6124
                     /locus_tag="PLZ75_02150"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOS71231.1"
                     /translation="MSDILIQNPTIINEGLSFRGDVLVSGEIIAAVGLPGEIEVPEKT
                     QILDASGLILIPGVIDDHVHFRDPGLTHKGDIFSETRAAAAGGITSFMDMPNTVPQTV
                     TNRDLDDKFSIASEKSLINYSFYIGATESNLDEILNTDPARVCGIKLFMGASTGNMLV
                     ENENSLRELFRQALIPVAAHCEDETTIRRNTASFRNKYGDAIPVEMHPMIRSRDACFK
                     SSALAVGLAREYNTRLHVLHLSTADEMKLFRPGKLNREKRITAEVCVHHLWFDDSDYA
                     KLGSKIRWNPAIKTIFDKNALTQAVNNDIIDVIGTDHAPHTPEEKSGSYTGSPSGAPM
                     IQHSLATMLELWHRKIFSLEKIVEKMCHNPAVLFNISKRGFIREGYKADLVLIDPEHE
                     WMVTKNNILYKCGWSPLEGQTFRSKIVKTFVNGNLVYDHGGINNDHRGQPLSFER"
     gene            complement(6165..7436)
                     /locus_tag="PLZ75_02155"
     CDS             complement(6165..7436)
                     /locus_tag="PLZ75_02155"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOS71232.1"
                     /translation="MKIYKFGGASVRDAAGIENLAEIVSAEKDDLVIVVSAMGKTTDA
                     LERVLNAWMKNDESYREHLDGILDYHLAVAKALLPPGHRGIKLIHNSFAKLGEYLSQE
                     EKSVYDYEYDQIVSHGEIWSTMLIAEYLNRRGLKVKWTDIRNKLVTDDRYREANILWG
                     ESYLRIRPAFDLPEYSCYLTQGFIGATIAGQTTTLGREGSDYTAAVLATILDAESVTF
                     WKDVPGILNADPRWLPDVEKLEEVSYREAVEMTFSGAKVIHPKTIKPLHNRNIPLYVK
                     SFIDPKGSGTLVTDEPKHRITIPVFVRKENQVLLSLLPKDFSFVMGDNLSRMFQAFIK
                     HGIKVSLIDASAVSINVCIDDERTKIDSLLNDLRNEYSAVYNDNVEILSVRHYTAEAL
                     DKIRKGREILLEQRSRGMVRLVTRQPVPERG"
     gene            7535..10957
                     /gene="mfd"
                     /locus_tag="PLZ75_02160"
     CDS             7535..10957
                     /gene="mfd"
                     /locus_tag="PLZ75_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOS71233.1"
                     /translation="MKQNGLTDLFNDHPVLSALTERIKSATEARMGVEGLYGSSKAVA
                     IAGAFLKTPFTHIAVVPEKEDAAYLYNDLVSLTGDDSVFFFPSTYKRSVLYEQTEPAN
                     IVLRTEVLNFLSSGGRKGIIVTYPESAMEKVISKKNLQKNTFTIRKGDNLSLEFLEEL
                     LREYNFERVDFVSEPGQYAIRGSIADIFSYSTDLPYRIDFFGEEIESLRTFSTDDQLS
                     VNTCDRISVIPNIQDISIEVTNDSFTAFLPETSVIWIEDTGYIRDKINDISGQLARKE
                     ESGQAEGKSATILNGGRFIEECLKFRTVEIGKPGLPVSCMRFEFRTEAQPVFNKNFSL
                     LSSKLVSNEEEGYRTFIISESQSQIDRLKDIFAEINPDLRFESMLLSLHGGFADHDIR
                     IAVYTDHQIFDRYHKFRIRGFFTKRDSISVKELTGLNPGDYVVHIDHGIGKFGGLEKI
                     EINGRIQEAIKLVYRDNDILYVGIHSLHRISKYKGREGAEPRIYKLGSGAWQKLKQRT
                     KSKVKDIAKDLIRLYAKRMASGGYSFSPDSYLQRELEASFIYEDTPDQLVATSAIKED
                     MEAPHPMDRLVCGDVGFGKTELAIRAAFKSAADNKQTAVLVPTTILAFQHYQTFTGRL
                     RGFPCTVEYLSRHRKPAEQKKIIADLESGKIDIIIGTHKLVGKNIRFRDLGLLIIDEE
                     QRFGVSVKEKLKTLRASVDTLTMTATPIPRTLQFSLMGARDLSVINTPPPNRMPIITE
                     IHDFNEEIIREGIEYEIARGGQVFVIHNRIENINEIRDKIKRICPSVRIAVVHGRMDG
                     DTIENVMFDFIRGDYDVLVATTIIESGLDIPNANTIFINEAQHFGLSELHQLRGRVGR
                     SNKKAFCYLLAPPLSALPQDARRRLKTIEEYSGLGSGFNIAMQDLDIRGSGNLLGGEQ
                     SGFIADVGFETYQNILSEALAELRYSETDESGDFGSGKAAGNVRTETENGGTPGQDNE
                     ARTLAARAVSGRTRSYVSDFQIDTDLEIMFPDEYISNISERIRLYKELNEIRDETALG
                     TFARNLEDRFGALPGPAAALLDIVRIKWLAVRLGMEKIMLKNDLLIANFVSDPDSPFY
                     RSPLFVSIMTYVNRFPGKMSVRQKETKLSLTVRDIRSVQSAIHALSNIPDSA"
     gene            11058..11780
                     /locus_tag="PLZ75_02165"
     CDS             11058..11780
                     /locus_tag="PLZ75_02165"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOS71234.1"
                     /translation="MNIIYDLGKAYNRLAMYNGSERIWCIHTKDLSCEQIMEKALPFR
                     LQKMIIHSSKELPEYIYDLISVDIPFVHLFTSKSKLPFTSHYFTAGTPDTGRIAAIAG
                     ACSHFPEQEILLISAGTVLTFDFLAAGKHEGTNISPGMSMRFKALQKHTGKRNFDSEP
                     GEYTSPGKNDAEAVKAGVITGIVYEINEYIRTFKKEHKKAAFVISGPDADLLGKRIRY
                     STRYMPELVMDGLNYILEYNAK"
     gene            11845..13053
                     /locus_tag="PLZ75_02170"
     CDS             11845..13053
                     /locus_tag="PLZ75_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71235.1"
                     /translation="MINSPYARFNLGTLEPAGSFRSRAMGGTGTAIRDNSTLYYTNPA
                     SFSSIDTNSFVFDFGINYGINTLSDGSEKYSSDDVNFDHMLLGLPLAKGWGAGFGITS
                     FSNGYYSIASKVGRNDSGYNPVTGEYAENHSGKGGISRLFAGTGVKMLRYFSAGVNVN
                     ILFGSIRRSNQFIFTDYFNSYNNDLTEKLELNGINFDYGLQAELPFKNNYFLIAGASL
                     GQGMNYRSRYEVLSYRYNYYGLNDTINWSADSSKTFIPGTLSLGLSFGKKNKFTAAVD
                     YVMTNWSESNLHGSRGYLADARSLSFGVEYIPEKYSNFSLFRRMEYRMGAHLDDNYLI
                     INGEQLKEAGITAGIGIPMRRSLSKANIYFDFTKRSGKGGIMHTENIFTIGASLNLYD
                     PYWFLKRKYD"
     gene            13113..13634
                     /gene="lptC"
                     /locus_tag="PLZ75_02175"
     CDS             13113..13634
                     /gene="lptC"
                     /locus_tag="PLZ75_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOS71236.1"
                     /translation="MILSAVFSACERKLDTIKNADILLLPSLTAKNSTTVFTDSGKIQ
                     LIMSFEIMESYNNVEQPYSEFRSGIRVSFFEGNDTPAGTVFSKYARYMDKKKLWELKD
                     SVVVVNEANEKLETEQLFWDQEKDLIYTDRFITITSEDQTVMGTGFESDHRLTRRKIK
                     NVKATIYLKEEEE"
     gene            complement(13814..14644)
                     /locus_tag="PLZ75_02180"
     CDS             complement(13814..14644)
                     /locus_tag="PLZ75_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71237.1"
                     /translation="MKNKILIRLFSLMAIISVMAACEKSPIEKAQDAYDATKVVPVVL
                     GFKKGPVKVLQTFSYEYEVSYYRAGSTWSWSAVDATISSVTPDTRGVNVLFDKLPASG
                     KAQIKVVETTSGGVKSPEKVIEVTVSPFCPLPVSGFVGTWTGKDVADDFEYSVTVTAS
                     LSDGKILLEGFGTDIIEEEAFWNEAVVEGGTCLVTINPDGTLVIPEQFFLDTDETEGY
                     KIKGEGTWDNCGPKPTLEIKYDIWYPDPDDNFWIADEYFGEPLTLTLTLAGDDDGNGG
                     "
     gene            complement(14751..16244)
                     /locus_tag="PLZ75_02185"
     CDS             complement(14751..16244)
                     /locus_tag="PLZ75_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71238.1"
                     /translation="MKTYKYFLLLIVTAAFLVTGCESLEVKNLNDPDFATAFSKPSDV
                     KGVAGGLINTWFINTQEYTGPALALWVGADAGTCSHGNMAMWDFSKEPRIAWDNTPSY
                     AYAAVTENFYKKMYSLLASSNEILGKVVNEGMKIEGDGGKDETPMVKAMAYLGQGLAL
                     GYVGLFFDKGFITTEETDLSLEIEHSPYGDLITAALAGLDKCITICESSTFTLPGPWI
                     PGKTYTQKEIGELANSMAARFLSYAPRNKEENEAVNWGKVLSYANKGLTYDFAPKMDD
                     VNWYDLYHTYANYQGWGRVDMRIAHMMDPDMPAQWVGADQYDHLPAPKTSHVDGVDDR
                     IFTDFEYLSSNTFRVERGYYHFSCYRYSRHDDYLATWITPSPAFRKAENDLLKAEAMM
                     HQNNFDGAAQIINAGERITRGALPPIAANAAEVKDAIFHERNIELFNSGMGIEFCTMR
                     KDDKLQKGTPLHFPIPGQQLEVNLMEPYSFGATKGVAGKDYSNGGWF"
     gene            complement(16264..19374)
                     /locus_tag="PLZ75_02190"
     CDS             complement(16264..19374)
                     /locus_tag="PLZ75_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS71239.1"
                     /translation="MKRLLLMIILCVFAGTSVLLAQARVITGTVTSSVEGEGPIPGVS
                     VTVVGTTLGTYTDLGGNYTLSVPANARFLSFSFIGMKTQDVEIAGRSVINVVLDQDLL
                     NLDEVIVTGVAAGTPKKKLAVSVERVDENKIKEVYAGSASSALQGKVAGLTVISSTGE
                     PGSAATILVRGATQITGSQAPLIILDGAIMEGTLGDINVDDIESIEVVKGASASALYG
                     SRAGNGVIAVTTRRGNLLTEGKSEIIVRNEYGYNSLAKKYNLATHHQYQLQDDQASFP
                     YTRYRGVTYPANYPGFGDEGVTGSRIIKADQYLDNPYARLYDHESEVFPGSGFYTNYI
                     SAGSNLGKTNFLISFENNRQAGLVTETDGYKRNSFRVNVDHRLTPKLLISASNLYVKS
                     STQDPGGANYYNGGVFFNTLLIEPDVNLKSENPDGQPYLFVPNKWQEEQENPIYNLWK
                     VEDLTKRNRILGTYNARYNISPAINVEGKYAFEYSVGDYSSYEPYDTWERGGSGPTYS
                     LGTYYRRGTNSFSQNFQTTVNFNQKFGDFNTRAKLSYLFEKQEYKSHSARGYDFSLGG
                     VPSFDAIAGNKSITSYQDEILAKNYFAILYLDYKDKYIFDGMYRYDGSSLFGSEARWN
                     PYYRLSAAWRLTEDIHIPNVQELKLRAAYGTAGQRPGFADQYEVMAISGGLTQKSQLG
                     NMHLKPSLSKEWELGLNFDFLNRFSFEVIRSITNTSDQILAVPLAVQYGGWPSQVQNA
                     GSLNSKVWEASFNAAVVRKTNFTYNAGFIFDRIRTKITELTVPPFQTGPQGQEADKCF
                     YIREGEVFGAMYGYKFLTSLEEMRQQLGADDNIDNYVLNSDGFVIRKGTEGTTEEKPI
                     KKLDEEGALWYGKTGDSNPKFKMAMTNNLSYKGIGLYALLEWKNGGDIYNKSAQWLTR
                     DDRHGMMDQSGKPDYLKKTLSYYKTFYDVNDFNDFWVEDGSYLKLREVSLSYTIPASA
                     LNNFANGYIKGIKVAVLGKNLWTLTNYSGYDPEVATTDGTQYFAYDFMGYPNYRSFAA
                     SLEIKF"
BASE COUNT         5576 a         4525 c         4361 g         5154 t
ORIGIN      
        1 gtcgaaccct tgaccccctg cgtgcaaggc aggtgctctg gccaactgag ctaattcccc
       61 aggtaatgta taatgtatta tagattatag attatagatt atagattata gactatagac
      121 tattttcgac attttaaata attgcacatc gcctataata cataatacat tatacattat
      181 acatccaagt agtcccgcgc ggagttgaac cgcggacctc tacattatca gtgtagcgct
      241 ctaaccaact gagctacggg actaattcag ttccggaatc cgggctgatt ccagccggtt
      301 tttatatcct ggaacgcgat attctctatc ctggtaataa ctgcttcatg ttttcaagga
      361 acatccaccg actatcataa tctccttcat ccggcccggc cattacacgg tggcaatccc
      421 cgcaaatgct tcctcctgtt tttccgaagc gactcagtgt cacgatgccc ggtccttcgg
      481 gaagaatttg catttaaaac gggtgcaaag atataaattg attcgttgtt tacaatatca
      541 aaatcaaaaa atattacatc accggtattc attaaacaat atccttctta acgaaaggta
      601 aaaataaggt tcatggtaaa attccatact gtcactattc cgatggcaat gagttttgaa
      661 aggtagaaat tcataccaag cctgtcatgc agaagataga tcaccagatt gttgatgcca
      721 agtccggcaa ccgaaatcag aataaaggaa aaatattcaa ttgctatttc ctcgctctta
      781 ctcccgaaag tccagatcct gttccatata tagttcgagg tggcggccag gataaatccg
      841 gtggaattgg caatgtattt gtttgccctt gctttttcct ttaaaagcca ggtgacgccg
      901 aaatcaatca gcattcccga aaaacccact acagaaaatt tcaggaactt cagtatgaca
      961 aacctgtcaa tcatcctttt ctattttcag ctttttaaaa ctgctgttca gtgaaggacc
     1021 gaaaagtgta ttcaacgaca ggccgaaact gtatttccct gcaggaagat caggaatgtg
     1081 tgccgggatc attccgtgta ccgtctctcc cggacctaca atattgacag gttcacaggg
     1141 aaatacatca tatatgtaaa gtattttacc ccttataaag accatgctga aactgacagg
     1201 gaaatgcctg tggttaaaat caatatcaaa ttcacaggga ttatgaaggg aaaaagacag
     1261 ctgtaatgta tcacctgaat gccggatccc ctcccctgaa tcaaattcta ttttcatcct
     1321 gttgacggtc tgcaggctgt cagttctgaa tccgccaaag cgcatcgtgt cggaaacata
     1381 tatctctgaa tttgtatgct gattgaggca gatgaaagcc gggcggttat gatatttctt
     1441 ttcaaactgc cagaggtcga actgtgtctg gcgcgaatag actgagctga cagccactgc
     1501 ttccccgcct gtaaaaaaat tgtaaagcga cggccgttga aatgcgcctg taaagaccag
     1561 cggaagaccc tctgcttcat cagccagctc acggtatttt atttcctttc cgccaaaagc
     1621 aatatccctt acaaagcgga tatcagtcat caggatcagc cttattgcaa caagaagcag
     1681 tattgacggc agaatgaacc ttccggcata gcgcagaagg gaaggatcgg agctgcattt
     1741 ttccgtaagg atcagtatca tcggtatcga acatgcaata gtccagtgcg gctcaacatg
     1801 tccgcggaac gacatcatcc agaaaaaaac aaggaaaccg gcaatctgaa ggtagtagtt
     1861 ccttttcatc aggctgtccg gcctgtacct gaacatgaca tataccacgg ctccgagggt
     1921 gaagggattg aagaccgcca tctggtttgg cagatactcc aggaaatagt tccaccggaa
     1981 tcctgcggac ctggtaacca ggtgatagcg gaagcttggg aaatcattcg acagctgcca
     2041 gtaaatatgt ggtgaaagga tcagaagggc aatcactcct gctgccagaa acctgtaatt
     2101 tttcagcagt ttcagattgg aaagcaccaa gaatcctata accagcacag cctgatactt
     2161 gctgtataca agtccggcca ttgatattgc aagcagaaga acctccttcc agccggcacc
     2221 ggcaaggaat ttcctgtatg caaaaaaggc aagtgcagtg aaaaacaaca ggggtgaatc
     2281 aggagcagcc aggacaccat aggctgagaa catacttatt gatgcagcca ctaaaaagaa
     2341 aatcacaacg gttctcctgt catctgtcct gaaatctgcc gtcagccaga tcagtataat
     2401 ggtgcctgtc tgcaaaagca ctgacataaa cctgatacca agattcccgg agaaaagaac
     2461 tgaacttatt cttgtcatca gtgcaaccat cggagggtgg tcataataac cccagtcgag
     2521 gtatctcccg aacaagccgt aataggcctc atcactcaga atttccataa aaaccgcctg
     2581 cagcaggttt acaagaaacc agattgcaag aaacagaatg aaaatacttc tgtaattcag
     2641 tttcctgttt acactatctt tcaggctatt ttcaggagtt ctcaattctt cattctgata
     2701 ttgttaagat tgaccgatac tttcagggcc tgttcattgt aaatattccc ttccgtaaaa
     2761 tgaaaggaaa actccagtct ggctttgata atattactcc tgagaaaact ccagtagaag
     2821 tctgtccggt tataggtatt tgtcctgtaa aacggatcac cccagtatag ctcgttgtta
     2881 tgaaccggat aatattccat ctgcccgtca ccggcataga atgtgttgaa aattcctatt
     2941 cccctgtact caatattaag ttcggacagg aatccgtggt gtaacagcca cccggttttg
     3001 agtgcacgtg ccctgtcaaa acctgtgaca taacccacat tgaagtcaag ccggctcagg
     3061 aaagtgactc ccgaaagatc cagtccggcg gatgtaagct gaagaagatt gtcatgaagg
     3121 ggttcatctg tgacaggatc catgaatccg gcaaaatgaa acatataact gaagttacgg
     3181 atgtagaaat gttcccgatt atatttcccc gaaaaaccga caaaaaatgt ctccctgctt
     3241 tcatatgact gacggctggt ccagtcaagc cataagttcg ctgatatgtt ccttccggaa
     3301 taatccagga ggataccgtt catattcggc cggtaaaagg agattgaatc cctgaaaaac
     3361 agtctgggaa acctgtcaag agcttcttcc ctcggaaatg caccagccag aaagctgaag
     3421 ccgctctttc tgaacatgta ataggccaca ggcgagagat catcaataat accggtgctc
     3481 ccgtattcat gaagcatatt tactccggca acaatggtat gaaccgaatc gaaactcact
     3541 gacatgcccg ggacaaatcg tacacctgcc atggtttgcg gaatatgaac acgggaatga
     3601 ccgaattcgg tattgtcgaa aaatgaatat aatcctgtcc tgaatccgat ttgctgtgac
     3661 ccagcattaa acggcattgc caggcacaat aatataatcc ccagtgagtt tttcattttc
     3721 tgacgattga cgggggacaa aattaaaatt aataataact taaaagctac atggtatgaa
     3781 gttttttatc tgaatttccg tattttagca atttaataaa accccatggc caatctgata
     3841 attataccca cgtatctgga aagtgaaaat attgaggcaa ttacacgaac cattttttcg
     3901 ctgagcattc ctttcagcat tctggttatt gatgacaatt cagctgacgg cactgcagca
     3961 attgtcaaaa accttcagac tgaatttaaa aaccttcatc tcattgaacg tcccggcaaa
     4021 ctcggactcg gaacagccta tattaccgga ttccggtggg cccttgaaaa cgacttcgac
     4081 tacatatttg aaatggatgc cgatttctcg cacaatcctg atgacctgat caggctgtac
     4141 agtgcgtgcc atgatgatgg tgctgacgtg gccataggat cgagatacat cagcggtgtc
     4201 aatgtggtga actggcccct ttcaagagtt ctgatgtcct attttgcttc agtatatgta
     4261 aggataataa ccaggatgaa tgtaaaggat acaaccgcag gatttatctg ctacaggagc
     4321 gaagtgctga gaaacataca tctggaacat atcagatcaa agggctacgg ctttcagata
     4381 gagatgaagt tcgccgcatg gaaactcgga tataaaatag ttgaagtccc gataattttc
     4441 accgacagaa agcttggcac ctcaaaaatg tcgggaggcg tattcaatga agcattatgg
     4501 ggagtcatcc ggatgaaaat acgaagctgg ttcatgaaat acagccccgg cggggcaggt
     4561 atgacagcgg atgcggaaac agaaaaggag gatgataaaa agaaggactg ataccattat
     4621 gcatttcccc cggcataaca gagaacaggg aaaaaagaca gccgggtacc ggaatgtctg
     4681 aatatccaaa ccctgtgaca gtcacacacc gggataaaca gacctgaaaa aacagaatat
     4741 aaagacggca ggataccagg actcaatcat ttgaatctta atgtcagata ttttaatcca
     4801 gaatcccacc attataaatg aagggctctc tttcaggggc gatgtgcttg taagcggtga
     4861 aatcatagct gcagtcgggt tgccgggaga aattgaggtt ccggaaaaga cacagatact
     4921 ggatgcatcc ggactgatcc tgataccggg tgtgattgat gaccatgtgc atttccggga
     4981 tcccggatta acccataaag gagatatttt cagtgaaaca agggcggcag cggcaggcgg
     5041 aatcacttcc ttcatggata tgcccaacac cgtaccgcag acagtaacaa accgggatct
     5101 tgatgataaa ttcagcattg cctccgaaaa atccctcata aactattcct tctatatagg
     5161 ggcaacagaa agcaacctgg atgagatcct gaataccgac cctgccaggg tgtgcggaat
     5221 caagctgttc atgggagcct caacgggcaa tatgctggtg gagaatgaaa actcgctccg
     5281 ggaattgttc agacaggccc ttattccggt agcagcacac tgtgaagacg aaacaacaat
     5341 cagaaggaat accgcatcat tccggaataa atacggagat gccattccgg ttgaaatgca
     5401 tcccatgata cgaagccgcg atgcctgttt taaatcttca gcccttgccg tcggcctggc
     5461 acgggaatac aatacccgcc ttcatgtcct gcatctctca actgctgatg aaatgaagct
     5521 tttcaggccc gggaaattaa accgggaaaa aagaatcacc gctgaagtat gtgtgcatca
     5581 cctatggttt gacgattcgg attatgcaaa gctgggatca aaaatcagat ggaatccggc
     5641 gataaaaaca atatttgata aaaatgcatt aactcaggca gtcaataatg acattattga
     5701 tgtgatagga actgatcatg cacctcatac ccctgaggaa aaatccggca gctatactgg
     5761 atccccctca ggcgctccca tgattcagca ttcactggcc acaatgctgg aactgtggca
     5821 caggaaaata ttcagcctgg aaaaaattgt ggagaaaatg tgtcataatc cggctgtttt
     5881 gttcaatata agcaaaaggg gatttatcag ggaaggatat aaagccgacc tggtgcttat
     5941 cgatccggaa catgaatgga tggtaacaaa aaacaacatt ctctacaaat gcggctggtc
     6001 gcctctggaa ggacagacat tcaggtcaaa gatcgtgaag acatttgtga acggaaacct
     6061 tgtttatgac catggcggga taaacaacga tcacagggga cagccgctgt catttgaacg
     6121 ctgaatacca gccggataat caccgcagga agtagcggcc ggcgtcagcc gcgttccggg
     6181 acaggctgcc tggtaacaag cctgaccatg ccccgcgatc tttgttccag aagtatttcc
     6241 cggcctttcc tgatcttgtc cagggcttcc gctgtataat gacgtacaga cagtatttca
     6301 acattgtcgt tgtaaacagc cgagtattca ttcctgaggt cattcagcag actgtcaatc
     6361 tttgtccttt cgtcatcaat gcagacattg atgcttaccg cacttgcatc aataaggctg
     6421 accttgatcc cgtgcttgat gaatgcctgg aacatccggc tgagattatc ccccatcacg
     6481 aatgaaaaat cctttggaag aagtgaaagc aatacctgat tctccttgcg gacaaaaacc
     6541 ggaatcgtta ttctatgttt tggttcatcc gtaaccagtg ttccggaccc cttcggatcg
     6601 atgaaggatt ttacgtaaag gggaatattt ctgttgtgaa gcggcttaat agttttggga
     6661 tgaatcactt ttgcacctga aaaggtcatc tcaacagctt ccctgtaaga tacttcttcc
     6721 agcttttcaa catcagggag ccacctggga tcagcattca gaattccggg cacatctttc
     6781 cagaatgtaa cactttctgc atccaggatt gtagccagga cggcggctgt ataatccgaa
     6841 ccttcacgtc ccagtgttgt ggtctggccg gcaattgttg cccctataaa accctgggtc
     6901 agatagcagg aatattccgg caggtcaaag gcaggcctga tcctcagata actctccccc
     6961 cacaggatat ttgcctccct gtatctgtca tctgtaacaa gcttgtttct gatatcagtc
     7021 cacttaactt tcaggcccct cctgttaagg tattctgcaa tcagcatggt tgaccagatt
     7081 tcaccatggg aaacaatctg atcatattca tagtcataaa ctgatttttc ctcctgtgac
     7141 agatactctc ccagctttgc aaaagagttg tgtatcagtt ttatgccccg gtggccggga
     7201 ggcagaagag ctttggcaac cgcaagatga taatccagaa tgccatcaag gtgttccctg
     7261 tagctctcgt catttttcat ccatgcattc agcacccttt ccagtgcatc ggtggttttg
     7321 cccatggctg aaaccacaat gacaagatcg tccttctcgg ccgatacaat ttccgcaaga
     7381 ttttcaatac ctgcggcatc cctgactgaa gcccctccga atttgtaaat cttcataact
     7441 gaaaatttcc cgtaaaaata agattaattc attattccgc ggccttgacg gggttccgga
     7501 aattgatatt aaatttgcat ccacatttgt gctgatgaaa cagaacggac tgacagactt
     7561 gttcaatgac catccggttc tttccgccct gactgaaagg ataaaatctg ccactgaagc
     7621 caggatgggc gttgagggtc tgtacggatc atcaaaagcg gtcgcaatag ccggggcatt
     7681 tctgaaaacg ccgtttacac acattgcagt ggttcctgag aaagaagatg ccgcctacct
     7741 ctacaacgac cttgtatccc ttaccggtga tgattcggtg ttcttcttcc cgtcaacata
     7801 caaacgctcg gttctctacg aacagaccga acctgccaat attgtcctcc gtacggaggt
     7861 tcttaatttt ctctcatccg gcggaaggaa agggattatc gtaacctatc ccgaatctgc
     7921 aatggaaaag gtgatcagca aaaagaacct gcagaagaat accttcacca tcaggaaggg
     7981 agacaatctt tcactggaat tcctggaaga actattgcgc gaatacaatt ttgaaagagt
     8041 tgactttgtc agtgaaccgg gtcagtacgc catcaggggt agcattgcag acatattctc
     8101 ctattcaacc gacctgcctt acagaataga cttctttggt gaggaaatag aatccctcag
     8161 aaccttcagc actgacgatc agctgtctgt gaacacatgt gaccggatat ccgtaatacc
     8221 aaacattcag gatatttcca ttgaggtaac gaacgactct tttaccgctt tcctgccgga
     8281 gacatcggtt atctggattg aagacaccgg atatatcagg gataagataa atgacatttc
     8341 cggacagctg gcaaggaagg aggaatcggg tcaggcagag ggaaaatccg ccacgatatt
     8401 gaacggcggg cgttttattg aagaatgtct gaaattcagg acagtagaaa taggaaaacc
     8461 aggactgccc gtttcctgca tgcgttttga attcaggacc gaagcacagc cggttttcaa
     8521 caagaatttc agccttctca gctccaaact tgtttcaaat gaggaagagg gataccggac
     8581 cttcattatc tccgaaagcc agtcccagat cgacaggctg aaagacatct tcgcggagat
     8641 aaatcctgat ctgcgttttg agtcaatgct tttaagcctt cacggcggat ttgcagatca
     8701 tgatatcaga atagcagtat ataccgatca ccagatattt gacaggtatc acaaattcag
     8761 gataagaggc tttttcacaa aaagggacag catctcggtc aaggagctta caggactaaa
     8821 tccgggtgat tatgtggttc atatagatca tgggatagga aaattcggcg gactggagaa
     8881 aatcgagata aacggcagga tacaggaagc catcaaactg gtttaccggg ataatgacat
     8941 cctgtatgtg ggcatccatt ccctgcacag aatttcaaaa tacaagggga gggagggcgc
     9001 tgaacccagg atatacaaac tcggttccgg ggcatggcag aagctgaaac aaagaaccaa
     9061 gagcaaagtc aaggatattg caaaggacct gatcagactc tatgcaaaaa ggatggcttc
     9121 cggaggatac tccttctcgc ccgattccta cctgcagagg gaacttgaag cctcgttcat
     9181 atacgaggat actcctgacc agcttgttgc aacatcagca ataaaggagg atatggaagc
     9241 accgcatccc atggaccgtc tggtttgcgg ggatgtgggt ttcggaaaaa cagaacttgc
     9301 aatcagggcc gcattcaaat ctgctgctga caacaaacag accgctgttc tggtacccac
     9361 aacaattctg gccttccagc attatcagac attcaccggc agactcaggg gttttccctg
     9421 tacggtcgaa tacctaagca gacacaggaa accggctgaa cagaaaaaga taatcgccga
     9481 cctggaaagc ggaaaaattg atataataat aggaacccat aagcttgtcg ggaaaaatat
     9541 cagattcagg gaccttggat tgctgatcat tgatgaagag cagagattcg gtgtttcagt
     9601 aaaggagaaa ctcaaaacac tcagggccag tgttgatacc cttacaatga cagcaactcc
     9661 gatcccccgg accctccagt tttcactgat gggagccagg gatctttccg taatcaatac
     9721 ccctccccca aacagaatgc ctattataac ggaaatccat gacttcaacg aggaaattat
     9781 cagggaagga attgaatatg aaatagcccg cggaggccag gtatttgtca ttcataacag
     9841 aattgagaat ataaacgaaa tccgggataa aataaaaaga atctgtccca gtgtgcggat
     9901 tgccgtggtt catggcagaa tggacggtga tacaatagaa aatgtgatgt ttgattttat
     9961 ccggggggat tatgacgttc tcgttgcaac cacaataatt gaatcaggac tggatattcc
    10021 caatgcaaat accatcttta tcaatgaagc ccagcatttc ggactgtcag aactgcatca
    10081 gctgagggga agagtgggac gttccaacaa gaaagccttc tgctacctgc tggccccccc
    10141 cctctctgca ctgccacaag atgcccggag aagacttaaa acgattgagg agtattccgg
    10201 actgggaagc ggattcaata ttgcaatgca ggatcttgat ataaggggat cgggaaacct
    10261 tctcggggga gaacagagcg gattcattgc cgacgtggga tttgaaacat accagaacat
    10321 tctcagtgaa gccctggccg aactgaggta cagtgaaacg gatgagtccg gagactttgg
    10381 atcaggaaag gctgccggca atgtaaggac tgaaactgaa aacggcggta ctcccggaca
    10441 ggataatgaa gcccgtacgc tggctgcccg ggccgtcagc gggaggacca gatcctatgt
    10501 cagtgatttc cagatagaca ccgatctcga aataatgttt cctgatgaat atatttccaa
    10561 tatatccgaa agaataaggc tttataagga acttaatgaa ataagggatg agacagcgct
    10621 cggcacattc gccaggaacc ttgaagacag gtttggcgcc ctgccggggc cggccgcggc
    10681 cctgctggat attgtcagga taaaatggct ggccgtcagg ctgggaatgg agaaaataat
    10741 gctgaagaat gatcttctga ttgccaattt cgtcagcgac ccggactcac ctttctacag
    10801 aagtcccctg tttgtctcaa taatgactta tgtaaacagg tttccgggaa aaatgtcggt
    10861 gaggcagaaa gaaacaaagc tgagcctgac tgtccgggac atcagaagcg tacagtcggc
    10921 aatccatgct ctcagcaaca ttccggattc agcctgagaa actgtttcgg ccctgataat
    10981 tcctgccagg tcctattgat ttacggcact ctggacaatt attttatatt tcctaacttt
    11041 gtaattattt taaagtcatg aatattatat atgatctggg caaagcctac aacagactgg
    11101 caatgtataa cggcagcgag aggatctggt gcatccatac caaagacctc agctgcgaac
    11161 agataatgga aaaggcactt cccttcagat tgcagaaaat gattatccac tcttcaaaag
    11221 aacttccgga atacatctat gacctgataa gtgtagatat cccttttgtt catttgttta
    11281 ccagcaaatc gaaacttcct ttcacaagcc attattttac tgcaggaacg cctgataccg
    11341 gcaggatagc agcaatcgcc ggtgcctgct cccattttcc cgagcaggaa atcctgctga
    11401 tcagtgccgg aactgttctg acatttgatt tcctggctgc cggcaaacac gaaggaacca
    11461 atatatctcc cgggatgtcc atgagattca aggcgctgca aaaacatacc ggaaagagaa
    11521 attttgactc tgaacccgga gaatatactt ctcccgggaa aaatgatgca gaagcggtta
    11581 aggcaggagt cattacagga atcgtatatg aaataaatga gtatattcgc acgtttaaaa
    11641 aggaacataa aaaagccgct tttgtcattt caggtccgga cgcagatctc ctcggaaaaa
    11701 ggatacgtta cagcaccagg tacatgcccg aacttgtaat ggacggatta aattatattc
    11761 tcgaatacaa tgcaaaataa gtttgctgca attattttta ttatcctaat ctcttccccg
    11821 ttttttagcg cgtcaggaca gaaaatgatt aactctccct atgcccgttt caatctgggc
    11881 acactggagc ccgccggatc attcaggagc cgcgcaatgg gcggaaccgg gaccgcgatc
    11941 agggataaca gcactcttta ttatacaaat ccggcatcat tcagcagtat tgacacaaat
    12001 tcatttgtat ttgatttcgg aattaattac ggtataaaca ctttaagcga cggttcggaa
    12061 aaatacagtt ccgacgatgt caattttgac cacatgcttc tcggattgcc tctggcaaag
    12121 ggctggggag caggtttcgg aataacatcc ttcagcaacg gttattattc tatcgccagc
    12181 aaggtcggca gaaatgattc aggatacaac cccgttaccg gtgaatacgc agaaaatcat
    12241 tccgggaaag ggggtataag cagacttttt gcaggtaccg gggttaaaat gctcagatat
    12301 ttctccgccg gtgtcaatgt caacatcctg ttcggatcca tcaggagatc aaatcagttt
    12361 atttttaccg actattttaa ttcatacaat aacgatttga ctgaaaagct cgagctgaac
    12421 ggaataaatt tcgattacgg tctgcaggcg gaactcccct ttaagaacaa ttattttctt
    12481 attgccggag cctcactcgg tcagggaatg aattacaggt cgagatacga agtcctgtcc
    12541 tacaggtata actactacgg actgaacgat acaatcaact ggtcggccga ttcttcgaaa
    12601 acctttattc ccggcactct cagtctggga ctgtcattcg ggaaaaagaa taaattcact
    12661 gcagccgttg attacgtaat gacaaactgg tcggagtcaa acctccacgg aagtcgcgga
    12721 taccttgctg atgccaggtc actctccttc ggagtagaat atattcccga gaagtattcc
    12781 aatttcagcc tgttcagaag aatggaatac aggatgggag ctcaccttga cgataattat
    12841 ctcatcataa acggcgaaca gctgaaagaa gctggtatca cggcaggaat agggattccg
    12901 atgagaagat ccctgtcgaa agcaaacatt tatttcgact ttacaaaaag atccggaaag
    12961 ggaggaataa tgcacaccga aaatattttc accatcggtg ccagcctcaa cctttatgat
    13021 ccctactggt tcctcaaaag aaaatatgat tgattttcat gtttcccctt ttttcccgaa
    13081 ataagctcca ctcaggggtc atttccgcag ttgtgatcct gtcagccgta ttttctgcat
    13141 gcgaaagaaa gctggataca ataaaaaatg cagatattct tcttcttcct tcactgacgg
    13201 caaaaaattc caccactgtt ttcaccgatt ccggaaaaat acagctgatc atgtcgtttg
    13261 agatcatgga gtcatacaat aatgttgaac agccttattc ggaattcaga tccggaataa
    13321 gggtgagctt ttttgaaggg aacgacacac cggccggaac agtgttttcg aaatatgcca
    13381 gatatatgga taaaaagaaa ctctgggaac tgaaggattc agttgttgtt gtcaatgaag
    13441 caaatgaaaa gcttgaaacc gaacagctgt tctgggacca ggaaaaggat ctcatctata
    13501 ccgacaggtt tattaccatc accagtgagg atcagacagt catgggtacc ggttttgaat
    13561 ccgaccacag actgacaaga cggaagatca aaaatgtcaa ggccacaatc tacctgaagg
    13621 aagaggaaga ataatatatc atgatcatac cggaacagca gggggtattc ccgaaacggg
    13681 aacaccccct gctgtttatc attgtatttt attcggttaa aacttcaggc acttccctgc
    13741 accggaaacc gtatatccgt caatcaaagt cctgatccag gatacttccg gtgcaaaggc
    13801 gtttccggct gaatcacccg ccgttgccgt catcatcgcc ggccagggta agggtaaggg
    13861 taagaggttc accgaaatat tcatctgcta tccagaaatt gtcatcagga tccggatacc
    13921 agatatcata tttaatttca agtgtaggtt ttggtccgca attatcccat gtcccttctc
    13981 cctttatttt atatccttcc gtttcatcag tgtcaaggaa gaactgttcc ggtataacaa
    14041 gagtgccatc cggattaatt gtcacaaggc aggttccgcc ttctacaacc gcttcgttcc
    14101 agaaagcctc ttcttcaata atgtcagtac cgaaaccttc aagcaatatt ttcccgtcac
    14161 tcagactggc agtaaccgta acactgtatt caaaatcatc ggccacgtcc ttgcctgtcc
    14221 aggtgccgac aaagccgctt accggaagcg gacagaaggg gctgacagta acttcaatga
    14281 ctttctcggg tgacttgaca ccgcctgatg ttgtttcgac gaccttaatc tgtgccttgc
    14341 cgcttgccgg gagtttgtca aaaaggacat ttactcccct ggtgtcggga gttactgaag
    14401 agatggtggc atcaacggca ctccagctcc aggttgaacc ggcacggtag taactgacct
    14461 catattcata actgaaagtc tgcagcactt ttacaggtcc ttttttaaaa ccaagaacaa
    14521 ccggaaccac ctttgttgca tcataggcgt cctgggcttt ttcaatggga ctcttttcac
    14581 aggcagccat aaccgatatt atagccatga gtgaaaataa cctgatcaat attttatttt
    14641 tcatttgtgg aattatttta gttttcataa ggtgatggaa acctgcatac cgatgcttca
    14701 tcccgtacag gtttccacta tcctgaattt attggctgta ttgttaaatg ctagaaccat
    14761 cctccgtttg aataatcctt tcctgcaacg cccttggttg ctccaaagga atagggctcc
    14821 atcaggttga cctcaagctg ctgaccggga atcgggaaat ggaggggggt acccttctgg
    14881 agcttgtcat ccttgcgcat ggtacagaac tcaatcccca ttccgctgtt gaaaagttca
    14941 atattccttt catggaagat tgcatcctta acctcggcag cattggctgc tatgggcgga
    15001 agcgctcccc tggtaattct ttctccggca ttgataatct gagctgcacc gtcaaaatta
    15061 ttctggtgca tcatggcttc tgccttgagc agatcatttt cagcctttct gaaagcgggt
    15121 gaaggagtaa tccatgttgc caggtagtca tcatgtctgc tatacctgta gcaggaaaaa
    15181 tgatagtaac ctctttccac cctgaaagta ttggatgaga gatattcgaa atctgtgaag
    15241 atacggtcat caacaccgtc cacatgtgag gttttgggag ccggaagatg atcatactgg
    15301 tcggcaccta cccattgagc cggcatatcc ggatccatca tgtgggctat cctcatgtca
    15361 accctgcccc agccctggta gtttgcatag gtgtgataga gatcatacca gttaacatca
    15421 tccatcttcg gggcgaaatc atatgtaaga cctttattgg catatgacaa tacttttccc
    15481 cagtttactg cttcattttc ctccttgttc cgcggggcat aggaaaggaa tcttgctgcc
    15541 atggaattgg ccagttcccc gatttccttc tgggtatagg tcttgccagg tatccacggt
    15601 ccgggaagcg tgaaggtgct gctttcacag atggtaatgc atttgtcaag gcctgcaagc
    15661 gctgcagtga taagatcacc gtagggagag tgctctattt ccagcgaaag atctgtttct
    15721 tccgtggtga taaagccctt gtcaaagaac agcccgacat aaccaagggc aaggccctga
    15781 cccaggtagg ccattgcctt taccatgggt gtttcgtctt ttccgccgtc cccttctatt
    15841 ttcatgcctt catttactac ttttccaaga atttcattgg aggaagccag cagggaatac
    15901 attttcttgt agaaattctc agtcacggca gcatacgcgt aagagggtgt attgtcccac
    15961 gcgatcctgg gttccttgct gaaatcccac atggccatgt taccatgtga gcaggtacct
    16021 gcatcagcac ccacccacag tgccagggcc ggccctgtat attcctgtgt gttgatgaac
    16081 caggtattta taagtccgcc ggccacaccc ttcacatcgg aaggctttga gaatgcagtg
    16141 gcaaaatccg gatcattcag attctttact tccaggcttt cacatcccgt tacaaggaat
    16201 gcagccgtga caataagcaa taaaaaatat ttatatgttt tcataatcgt aatattctgg
    16261 atgttagaac tttatttcaa gtgatgcagc gaacgacctg tagttcggat atcccatgaa
    16321 atcatatgca aaatactggg taccatcagt ggttgctacc tccggatcat atcctgaata
    16381 gttggtgagg gtccagaggt tcttgcccaa tactgccact ttaattcctt tgatgtatcc
    16441 gttggcaaaa ttgttgagtg ccgaagccgg aattgtgtag gaaagcgaca cttccctcag
    16501 tttcaggtag cttccgtcct caacccagaa atcgttgaaa tcgttgacat cataaaaggt
    16561 cttgtaataa ctcagggtct ttttcagata atcaggcttg ccggactgat ccatcattcc
    16621 gtgcctgtca tccctggtaa gccactgtgc cgacttattg tatatgtctc ctccgttttt
    16681 ccattcgaga agtgcataaa gacctatacc cttatagctt aagttattgg tcattgccat
    16741 cttgaatttg ggattagagt ctcctgtttt gccataccac agtgcacctt cctcatcgag
    16801 tttctttatc ggcttttctt ccgttgttcc ctctgttcct ttgcgaatga caaagccgtc
    16861 gctgttgaga acataattgt cgatgttatc atcagctccg agctgttgcc tcatctcctc
    16921 gagtgaggta aggaacttat atccatacat tgcaccgaat acttctcctt cacggatata
    16981 gaagcatttg tcggcttcct gtccctgagg accggtctgg aaaggcggaa cggtcagttc
    17041 agtaatcttg gtcctgatcc tgtcaaagat aaatccggca ttataggtga aatttgtttt
    17101 tcttacaact gcagcattga atgaggcctc ccataccttt gaattaagcg accctgcatt
    17161 ctgcacctgg ctgggccatc ctccgtactg gactgcaagc ggaacggcaa gtatctgatc
    17221 agaggtattt gtaatcgatc ttattacctc aaaactgaac ctgttgagga agtcgaaatt
    17281 taatcccagt tcccattcct ttgaaagaga aggcttgaga tgcatatttc ccagctgtga
    17341 tttttgcgta agaccgccac tgatagccat aacttcatac tgatcggcaa atccgggtct
    17401 ctgacctgct gttccgtatg cagctctcag ctttaattcc tgaacattgg gaatgtgaat
    17461 atcttcggtt agcctccatg ccgcggaaag cctgtagtag ggattccatc ttgcttcgga
    17521 accgaaaagg gatgatccgt catagcggta cataccgtca aaaatatact tgtccttata
    17581 gtcaagataa aggatggcga aatagttttt tgcaagaatc tcatcctggt agcttgtaat
    17641 ggacttattc cctgcaatgg catcaaagga gggaacgccg cccagactga aatcatagcc
    17701 tctggctgaa tggctcttgt attcctgctt ttcaaacaga taactcagtt ttgcccttgt
    17761 attgaaatct ccgaacttct gattgaaatt taccgtagtc tggaaattct gggagaaact
    17821 gtttgtacct cttctgtaat aggtacccag ggaatatgtg ggtcctgatc cgccacgttc
    17881 ccatgtatca taaggctcat aacttgaata gtctccgacg gagtattcaa aggcatattt
    17941 cccttcaaca tttattgccg gggaaatatt gtacctggca ttatatgttc cgagtatcct
    18001 gttacgtttt gtaagatctt cgactttcca aagattgtaa atagggtttt cctgctcttc
    18061 ctgccatttg ttaggtacga aaagatatgg ctgtccgtca gggttttctg atttcagatt
    18121 cacatcgggt tcaataagaa gagtattgaa gaacacacct ccgttataat agttggctcc
    18181 tccgggatcc tgtgtcgatg acttcacata aagatttgaa gccgatatca ggagtttggg
    18241 agtcagcctg tggtcaacgt tcaccctgaa gctgtttctt ttgtatccgt cagtttctgt
    18301 aacaagtcct gcctgccggt tattttcaaa agaaataagg aagttggtct tgcccaggtt
    18361 tgatccggct gaaatatagt ttgtatagaa tccgctgccg gggaacacct cactttcgtg
    18421 gtcatagagg cgggcatatg gattatcgag gtactgatca gccttgatga tccgggatcc
    18481 tgtaacgcct tcatcgccaa atccaggata attggcagga taagtcactc ccctgtatct
    18541 ggtatagggg aatgaggcct gatcatcctg aagctgatac tggtgatggg tggcaagatt
    18601 atattttttt gccagtgaat tgtaaccgta ttcgttcctg acgatgattt cagatttgcc
    18661 ctcggtgagg agattgcccc tgcgtgtggt aacggcaata acgccgtttc ctgcacgtga
    18721 accgtaaaga gcagatgcag aagcaccctt tacgacttct attgattcaa tatcgtcgac
    18781 attgatgtca ccgagcgtac cttccatgat ggctccgtcc agaatgatga ggggggcctg
    18841 tgatccggtg atctgggtgg caccgcggac aaggatggtg gctgcactgc ccggttcccc
    18901 ggtggagctg atcactgtaa gaccggcaac cttgccctgc agggcggaag aggctgatcc
    18961 tgcataaact tccttgatct tgttttcatc aacacgctca accgatacag caagcttctt
    19021 cttgggagta ccggcggcaa cacctgtgac gataacctcg tcaaggttaa gaaggtcctg
    19081 atccagcact acattaataa cgcttctgcc ggcaatttcc acatcctgcg tcttcatccc
    19141 gataaacgag aagcttaaaa atcttgcatt tgcaggcaca ctcagggtat aattccctcc
    19201 cagatcagta taggtcccca gtgtggttcc cacaactgtg acggatactc cgggaatcgg
    19261 tccctctcct tctacggaag aagtaactgt acctgtaatc actcttgcct gtgccaacag
    19321 aacggaagtt cctgcaaaca cacacaaaat aatcatcagt aatagtctct tcataactaa
    19381 ttcagtttta ggttttgaac atagggcaca aatatacttg ttttttcttt ttttcaagaa
    19441 tagcaacagt aattttttaa atatcaatca aaatcgcagt ttttaagtat taaaaaatgt
    19501 taaaaatgct ttgcattttt aatatatata acatttggta tgattcagga ttctttccgg
    19561 agttctctgg cagcagcaag cagtttctca tacttttcct tccccagcgc ccttat
//
LOCUS       DAOSPX010000022        19262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3014_length_19262_cov_4.584735, whole genome shotgun sequence.
ACCESSION   DAOSPX010000022 DAOSPX010000000
VERSION     DAOSPX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19262
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3014_length_19262_cov_4.584735"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..342)
                     /locus_tag="PLZ75_02195"
     CDS             complement(40..342)
                     /locus_tag="PLZ75_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOS71240.1"
                     /translation="MKQIITASSNIKHRNLKPGDQYSPASLQKILKQSVLKAGIRKPV
                     TLHWLRHSYATHLADSGTDLRYIQELPGHKSSRTTGIYTHVSVSSIQKIRSFFDDL"
     gene            complement(381..950)
                     /locus_tag="PLZ75_02200"
     CDS             complement(381..950)
                     /locus_tag="PLZ75_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71241.1"
                     /translation="MDHTESLKNLVLAPYIIKATALIGKSRNVGGNQFRHAIATLGIL
                     LDYKYFDNYVLLKASVIHDLFEDIPETNEYEIRSIDYEADMVVDLVLEVTRPEGMPKV
                     EYLKRILEHGSVNAKILKSADRISNLTDLHRAQYSRKVMSEYLDMTEEWVLPMAESVN
                     PDMAIELGDLIRKRREQVNILKYPGLLQL"
     gene            complement(1091..1657)
                     /locus_tag="PLZ75_02205"
     CDS             complement(1091..1657)
                     /locus_tag="PLZ75_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71242.1"
                     /translation="MDIPFQVPEPFLFSPLKHYFPYIRAYSEKKAAVERYPGSPEFIR
                     ELRQIGSCVMDVYRGELTADRIFSEISNFLISRGISRKEAYAAWTGKSFSSYRKINLA
                     DSSEWILKYNDDDTRYVHLFPSRSGPFTFRVKASTLRSALLYQILIGKDYITGEDLNR
                     ARFPAGLPPVKSIEDAGAITEMIEMLRD"
     gene            1786..3042
                     /locus_tag="PLZ75_02210"
     CDS             1786..3042
                     /locus_tag="PLZ75_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS71243.1"
                     /translation="MTPGSKRNFTDTINHRQPERLVVDFGSTSVTGIHVLIVEKLREY
                     YGLEKRPVKVTEPYQMLGEIDNELIHAMDLDVLGLYGENNMFGIRNRGWKVHRTPWGQ
                     EVMFPGEFNYSFTEKGDILIHPEGDTSVPPSAVMPVSGYFFDALHRQEPIDDATLRPE
                     DNLEEFQAVTDHDLDFWKSQLESLDYSGKAVIASFGGTALGDIALVPGLQLKNPRGIR
                     GVEEWYVSTVTREEFIRNLFDRQTDIALENLKRIYGVIGDRIDAVFICGTDFGTQDST
                     FCSPETFSRVWMPYYRKVNDWIHDNTGWKTFKHSCGAIESFMELFIESGFDIINPVQI
                     NASGMDPKMLKKKYGGRIVFWGGGVDTQKAFMFGTPSQVRDQVRQMCDIFNDGGGFVF
                     NTVHNVQANVPFENVVAMLEALREIR"
     gene            3179..4285
                     /locus_tag="PLZ75_02215"
     CDS             3179..4285
                     /locus_tag="PLZ75_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS71244.1"
                     /translation="MKKIIFTFVVTVFVLHGCTGQGDRQTREDRDDPGKIMLKDYRPV
                     SLYNIPVTYIEKAAFPVFDAHSHDYATNREEIEQWIKNMDECGIARTVVHTQASGAIF
                     DSIYNLYSPYGDRFQLWCGFDYTGYDESGWGPAAIAELERCHRLGARGVGEEGDKGMG
                     LTFNRPVAARGMHFDDPRMKPLFEKCAELNMPVSIHVADPIWMYEKMDSTNDGLMNGY
                     KWRINTDRPGIIGFDALIQTLENAVRDNPKTTFIACHFANLNHDPDRLGQLLEKYPNL
                     YADISARFAESAPIPRYMKAFYEKFQDRLLYGTDWGYETEMYRKTFRILESNDEHIYE
                     IDHFNYHWALSGFGLPPGILKKVYYDNAAGLFGD"
     gene            complement(4294..5211)
                     /locus_tag="PLZ75_02220"
     CDS             complement(4294..5211)
                     /locus_tag="PLZ75_02220"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOS71245.1"
                     /translation="MNIQQFQYVLAVAEHRHFETAAEKCFISQSTLSTMISKLEDELG
                     VIIFNRKKKPVDITREGQEIIAQLRVINFEITQLNELVKKMTGEVTGILKIGCIATVA
                     PYLLPLILYDFSVKYPGLSLEIKESTTDEIVSLIKSRELDTGIISTPLNDKELIEYPV
                     YREPFVLYDTSGIHSGGCSLRDMSWDNFWLLEEGHCLSDQILEICSSGNSYIKSPSSI
                     HFKTGSIDSLVRFVKTYKGQTLLPSLAVRNFREEDRRHLVRFVQPAPSRTIGLVVHQH
                     FANKQILELLQNEIRLKTALLNDIEVISP"
     gene            5299..6765
                     /locus_tag="PLZ75_02225"
     CDS             5299..6765
                     /locus_tag="PLZ75_02225"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOS71246.1"
                     /translation="MAEKKKKLTSTSGRPIADNQDVKTAGKRGPMLMEDYWFLEKLSH
                     FDREVIPERRMHAKGSGAFGKFTVTHDITKYTKAKLFSEIGKETEVFVRFSTVAGERG
                     AADAERDIRGFAIKFYTEEGNWDLAGNNTPVFFLRDPYRFPDLNKAVKRDPKTNLRSA
                     NHNWDFWTLLPESLHQVTITMSDRGIPKSYRHMNGYGSHTFSLINADNERIWVKFHFK
                     TEQGIENLTNEEAAAIIAKDRESHQRDLFNAIERGDYPRWRMKIQLMTEEQAEKSPFN
                     PFDLTKVWPHADYPLIDVGVLELNRNPENYFADVEQAAFNPADVVPGIGFSPDRMLQA
                     RLFSYGDAQRYRLGVNYYQIPVNKPKYPFHNYHRDGAMRVDGNEGSTIHYFPNSYGQW
                     EESREYSEPPLKLSGDADHYNFREDDNDYYSQAGNLFRLMSPEEQKRLFCNTAAEVGG
                     AEKFIQERHIVNCYKADPAYGKGVADALGIDINTVKIE"
     gene            7104..10160
                     /locus_tag="PLZ75_02230"
     CDS             7104..10160
                     /locus_tag="PLZ75_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOS71247.1"
                     /translation="MSFGKRLPIYCRNYFRTCFTALSGARRLTVILLLSVTAVAKAVR
                     PESVYTQRPDDPEAFYFTPENYGFKAGGKSDVSEALQRAINQVKAEKNFGILFIPEGK
                     YRISRTIYIPGAVRLIGYGSKRPEIILTRNSPDFQKEAEDDKGRAAYMFWFTGGPVSE
                     GRKPQDAGAGTFYSAMSNIDLRIEDGNPYAVALRTHYAQHSFVSHMNIYAGSGKAGLF
                     DAGNEMENVAFYGGDYGIYTTKASPGWPVMMVDSYFEGQRKAALHTQESGLAIVNMHV
                     RNVPCVIEIEPNYADRLFIEDSRFENVRGPALVISNENNSNNQITLRNVDCRNVPLLA
                     RFRRSDTETPVNHRMYRINNYWHGLQMDDMLDQPRYRTVADIIPLESWSSEISSVTVP
                     SLPPVSSWVNILELGACGDGKTDNTRIIQDAIDMYDNIYIPQGWYLVSNTLRMKLNTC
                     LIGLHPMGTQLMIKESTAAFSGFGPPAAILESSRGGRNILNGIGINTNTFNYRAVGLK
                     WMAGPESLVNDVKFTGGHGSMQKPGEPASRTVYTSQGMDPSWDNQYWSLWVTGNGGGI
                     FKDIWTASTHATNGLYVSNTSSPGRIYAMSVEHHVRNEVRMKNVSNWKIYALQLEEES
                     RESTECQPMELENCRDLTFANLYMFRVIRINRPYHNSVRIWNCENVEMLNLHNYSQIK
                     YTTDIPVYDINRDIEVRPWELQRLVISGSEKRPVPLQAVPGVAEKLAGGFEFIEGITR
                     DSRGNIYFSENRMRRIYRWSAENNTLSMVADFPWKPVSLGCDSLDNLLVVFRYDPQPG
                     HMIDGKPESYYFPELPQEDRGGTSFSGWGNSGFAIRVYSIDPDNPEETISLLPLVPMK
                     SVASVHKALYVSNRWRDFHDFNSISLRVPPDCFLAPDKRTIIPRHYDLARGSSLLEAF
                     PGKPFYTSDEYDRRMVRFDVAGDGTLSNLHYFTEWGEFGSATDSEGNLYVADGNILIF
                     DKTGRMQGTIQVPERPSTIQFGGRNGDQLFITGRSGFFRVIMEKPD"
     gene            complement(10252..11163)
                     /locus_tag="PLZ75_02235"
     CDS             complement(10252..11163)
                     /locus_tag="PLZ75_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOS71248.1"
                     /translation="MKLKSKYKNLLLITFLFITAAVPLRPVHAQATDPEGFHILKKGS
                     SMKFFRYRPGAPVIISGHRGGREKGYPENSLEGFRNVLRQMPAFFEIDPRLTRDSVIV
                     LMHDATLDRTTTAKGNLNDYTYEQLRDVRLRDFAGNPTAFSIPTLEEVIVWSRGRTVV
                     NLDRKDVPPEMIVDLVKRLRASGHVMLTVHNGAQAKYYHENLPKAMLSAFVRTMKEYE
                     NIASSGVPWKKMIAYVGPAITSENAELVRLLHSRGVRCMISVAPTHDRLQEREERRKG
                     YLQELASNPDIIETDLPLEVWNVMKEK"
     gene            11459..13774
                     /locus_tag="PLZ75_02240"
     CDS             11459..13774
                     /locus_tag="PLZ75_02240"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOS71249.1"
                     /translation="MKKQKTVNLFLLLLSLLLASLSCRQGSSDTGTANIDNGLISIAF
                     NDRNGSLVNFSDISGKHEWIDDDVAAGSPWELYFTEASGIEPINAADAGKFRFSKPDS
                     RTLILKWKRFGNPGIRNLEVEARVSLDSDKALSYWNISVRNPDRKQIRQLNYPKLAGL
                     REAGEEYLAVPQWMGQITKNPRESLAPGVSAAGRYEWTYPGLSLQCLALYSPGRSGLY
                     IACNDTLAYIKNFSFTLDSLNTLTYRMHNFPSIDPSSASYTIPYSAVVGSFRGDWITA
                     AEIYREWASGQRWARESRFLKGLTPEWLEKTALWVWNRSKSDNVLVPAADIRQRLGLP
                     VSVFWHWWHGCSYDDGFPEYLPPREGRKSFLSAMDEAHKKGIRSIVYMNQRLWGTETE
                     SWEKENAAEYSVKNSDGSMNTHVYNIFSGKGTASMCLGTAFWKDRYASLCDSVVNSYT
                     ADGVYMDQACLALPCYDPGHDHPTGPGNYWVENFGRLTDQIRSKVAGEKQMILAGEGC
                     GEAWLPHLDLMLTLAVSKERYAGLNQWETIPFFQAVYHQYSITYGNYSSLLVPPYDEL
                     WPEEYAPEDPLEMLDRKFSRQFLMEQARSFVWGMQPTIANYHARLASHRKEEIDFLLR
                     LSRIRNNNLKYLLHGKFMRSPDIGSPSEEIDISRLSIYAGKTGNTVSAFRGTFPLVYS
                     GAWKADDGDLGIALASTGDRPVSLSFGFDAGEYGLPAAGRIFLADDRGRRELTAYTDS
                     RAEISLTLAPAAVCVLEVIPDHGSSSGNEMQ"
     gene            complement(13790..16042)
                     /locus_tag="PLZ75_02245"
     CDS             complement(13790..16042)
                     /locus_tag="PLZ75_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71250.1"
                     /translation="MKQLSLLFLIAGFVLTTACNQKNSVSDGDIIIENDQARLVISAD
                     GTTKSLVYRPGKTECLIQGGKIPFSTITEPRPYQNEVKLAYPNKKTTFRSNSVRQEGD
                     RLIIGYELIPWEATVSFRVTADYIAFTLEEFNLTEPYGITMTEPPISEMWFLRLPVRN
                     LGHWGDWLNVIWNEKVAVCVLAAEPCANADSEEGDGYRILQAGVDEKVKLTDVTAVLL
                     TCSRDQLLDKIAVVEKDYGLPAGADSRRSNLYNASYYWTSNITPLNADEHIKYARMGG
                     FRLMNIYYPAFLESRGYRLIGNYDVYRPEYPNGKDDLKAMLQKIKDAGITPGCHFLHS
                     HIGRDSRYVTPVADHRLNLLRVFTLAKPLGKTDTVIYVEQNPQNSTMAGNRKVLKIGG
                     ELVSYETYTTTPPYSFRGCVRGIDNTGTGAHPAGYMFGLLDVSEFGATSVYIDQYSGL
                     QDEIAGYIADLWDAGFEFIYFDGSEGVNPPFWFHVANAQWRVFSKLKPEPIFAEGAAK
                     THFSWHMLSGGNAFDVFPPEKLKEETIKHPFREAPRMRDNLTRLNFGWLGYWLPDEKT
                     IGTQPDQLEFVTSKAAAWDCPVSIHAYPATLARHPRTEDNFEVFRRWEEVRARKWLTE
                     DQKKMLQDPDQEFMLLLNEKNDFELVACDQIEDVAGGSREVIAFTFRRNRDLYACYWH
                     ISGDRKLELPLRAADLTLMENLGSTIRVQAAENDAGTVLPAGKRRYIRVTNMKLSDLQ
                     TAFRNARIID"
     gene            complement(16319..17128)
                     /locus_tag="PLZ75_02250"
     CDS             complement(16319..17128)
                     /locus_tag="PLZ75_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71251.1"
                     /translation="MAERIVRKTDEKVVSAVKDDHISLSSGGTEVLINLKNGLIAGAS
                     RDGRSSNFTDGPRFVGISSVPSEVRHYESPDGYVVEALYPGRSGGSVKWTMLPGGWLR
                     LDYGYRPAGLQDLLGITFSFSEETVKDAFLMANGPYRVWKNRLKGPLFGVYHKEYNNT
                     VTGESWDYPEFKGYYSNFYAVRIGTADLPLTIVSSTNDIYLHLFTPQNPGHASGWVTP
                     EFPEGNISLLNSISPIGTKFNPPESLGPGSQKNSYREAKPLGGTIYFRFGD"
     gene            complement(17110..19074)
                     /locus_tag="PLZ75_02255"
     CDS             complement(17110..19074)
                     /locus_tag="PLZ75_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS71252.1"
                     /translation="MKKITGLLTAILLIAAANGQKAEIVYLSGTDNENTVLWDFFCTG
                     GQNSGKWTKIPVPSNWELQGFGTYNYGNDKQQADEKGLYRHEFTVPAAAGKRRIFLVF
                     EGSMTDTEVRVNGKPAGTVHQGAFYRFSYEITSLIKPRGSNLLEVTVSKKSADESINR
                     AEREADYWIFGGIHRPVYLKIVPYEFIEHVAVNAEADGDFTAELTLKGCSGTRSIEAM
                     IMTPDGRAVAGPFTTGVKKGQTRVLIRGKADKPKQWTPESPDLYRAVFRLKDKDAILY
                     ETEERIGFRTVEVRQNDGIYVNGVRVMFRGICRHNSWPSSGKTTSKKISILDVNLMKD
                     MNVNAVRMTHYPPDKHFLEVCDSLGLFVINELSGWQAYYDTGPGRKLVRELVERDVNH
                     PCIILWANGNERGFNPELREEFAKYDPQKRLVIEPISNFNGMDTKHYIPYNYGVSTFF
                     NGRDIFFPTEWLHGCYDGGSGAGLDDYWNLMLSNPLSAGGFLWLFADEGVVRTDRKDS
                     IDTAGNQAPDGIVGPYREKEGSWFTVRDVWSPLWLERKIITPEFDGRLKIENRFLYTS
                     LDKCRLSYYLIKFSDSFPVSVTQSEKQQIDIPPLRPGQRDQVQLSLQAGWNDYDAIRI
                     DAEDPSGRLINSWSFGHSILPARRRWLKEL"
BASE COUNT         5005 a         4316 c         4725 g         5216 t
ORIGIN      
        1 gtagctgggg tgaattttat attttgtacg gccgggaagt cagagatcat caaaaaacga
       61 tcttatcttc tgaatgctgc tgacggatac atgtgtgtaa atcccggtgg tccgcgagct
      121 cttgtgaccc ggcagctcct gaatgtagcg caggtctgta ccgctatccg ccagatgagt
      181 ggcatagctg tgccgcaacc agtgtaaagt aacaggcttg cgtattccag ccttcaggac
      241 agactgcttc aaaatttttt gcagactggc tggggaatat tgatccccgg gcttcaggtt
      301 acgatgcttt atattggaag aggcagtgat gatctgcttc acctcctcct tgctcagaac
      361 acagggcagt gcagaattta ttacaactgc agcagtcccg gatatttcag tatgttcacc
      421 tgttccctcc gttttctgat gagatcgccc agttcgattg ccatgtccgg attaacactt
      481 tctgccatcg gcaataccca ttcttcagtc atgtcaagat attcggacat caccttcctg
      541 gaatactggg cacggtggag atcggtcaga tttgatatcc tgtcggcaga cttcagtatc
      601 tttgcattta ctgagccatg ttccagaatg cgcttcaggt attcaacctt tggcataccc
      661 tccggccggg ttacctcaag aacgagatcc accaccatgt cagcttcgta gtcgatgctg
      721 cggatctcat actcattcgt ttcaggaata tcctcgaaca ggtcgtggat cacagatgcc
      781 ttgagaagta cataattatc aaaatacttg tagtccagca gtatgcccag ggttgctatt
      841 gcgtgcctga actggttgcc gccgacattc ctgctcttgc ctatcagggc agtggctttg
      901 ataatataag gagcgagtac aagatttttt aaactctcgg tatgatccat tggtattgag
      961 attcagatct gtaaatttag caatttttcc ccttcatacg gctttccgga gcctgtagtt
     1021 ttcaaacctc tcagaatgaa tttattatat cagctaccgg aaggggccgt ccggctgaat
     1081 tatgtgaata ctaatcccgt aacatttcta tcatttccgt aattgcacct gcgtcctcaa
     1141 tactttttac aggtggtaat cctgccggaa atcttgcccg gttcagatct tctcctgtaa
     1201 tataatcctt gccgatcagt atctggtaga gtagtgccga cctgagggtc gaggccttta
     1261 ccctgaaggt gaaaggacct gatcgtgacg ggaaaaggtg gacatacctg gtatcatcat
     1321 cattatattt gagtatccat tccgaactgt cagcaaggtt tatcttcctg taactgctga
     1381 aactttttcc tgtccatgcg gcatatgcct ctttccggct gatcccccgg gaaatgagaa
     1441 aattgctgat ttcgctgaaa atacgatccg ctgtcagctc tcccctgtag acatccatta
     1501 cgcatgagcc gatttgccgt agttccctga taaattcagg agatcccgga tatctttcca
     1561 ccgctgcttt cttttccgaa tatgccctga tatatggaaa ataatgcttc agcgggctga
     1621 aaaggaaggg ttcaggcact tgaaacggaa tatccatgag ctggtaaaaa tgtgaaaatg
     1681 aaactatttt tcgatgaaaa caaaaagcag gcagaaaaat aaggttgaat aataactgaa
     1741 agatttaact ttattggttt aataacgttt ttaaattcca attcaatgac gcccggatca
     1801 aagagaaatt ttaccgatac catcaatcac cgtcagccgg aaaggctggt tgtggatttc
     1861 ggatccactt ctgtaacagg aattcatgtg ctgatagttg aaaaactccg ggagtactac
     1921 ggactcgaaa aaagaccggt aaaggtgact gaaccatatc agatgctcgg ggaaatagac
     1981 aatgagctga tacatgcgat ggatctggat gtccttggac tttacggtga aaacaatatg
     2041 ttcggtataa gaaacagggg ctggaaggtg caccggactc cctgggggca ggaagtgatg
     2101 tttccgggcg aattcaatta tagctttacg gagaaagggg atatacttat tcatcctgaa
     2161 ggggatacat cggttcctcc cagtgcagtg atgcctgttt caggttactt ttttgatgcc
     2221 cttcacaggc aggaacccat tgatgatgcc actttgcggc cggaagataa tctggaagaa
     2281 ttccaggcag ttacagatca tgatcttgat ttctggaaaa gccagcttga atcgctggat
     2341 tactccggga aggctgttat tgcatctttt ggaggaactg cacttggaga tattgccctt
     2401 gtaccgggtc tgcagcttaa aaatccccgg ggtatcaggg gcgtggagga atggtatgtg
     2461 tcaacagtga cgcgcgagga attcatcagg aacctgtttg accgccagac agatattgct
     2521 ctggaaaatc tgaaaagaat atacggtgtg ataggagaca ggatagatgc cgtgtttatc
     2581 tgcggcactg atttcggaac ccaggattcc acgttctgtt ctccggaaac cttttcgagg
     2641 gtatggatgc catattacag gaaggttaat gactggattc atgataatac cggctggaaa
     2701 actttcaagc attcatgcgg tgcaatagaa tccttcatgg aactgtttat cgaatcggga
     2761 tttgatataa tcaatccggt tcagataaat gcttcgggaa tggatccgaa gatgctcaaa
     2821 aagaaatacg gcggaaggat tgtgttctgg ggaggaggcg ttgataccca gaaagcattc
     2881 atgtttggaa cgccttcaca ggtaagggat caggtaagac agatgtgtga catattcaat
     2941 gacgggggag gttttgtgtt caatacggta cacaatgtgc aggcgaatgt gccctttgag
     3001 aatgtggtgg caatgctgga ggccttaagg gagataagat gaatgtaaac attaaggctg
     3061 cccatgaccg gcatctcgtt atccataatt ttgcggttcc ggaaactgcg ccggggattg
     3121 aacggcttac ccggaaatat ttgatttact tttaatacca aaccatatta acaggcttat
     3181 gaaaaaaatc atttttacat ttgttgttac agtttttgtt ctgcatggct gcaccggcca
     3241 gggagaccgt cagactaggg aggaccggga tgatccggga aaaataatgc tgaaggatta
     3301 ccggcccgtt tcattgtata atattccggt aacctatata gaaaaggcag ctttcccggt
     3361 gtttgatgcc cactctcatg attatgcaac aaacagggag gagatagagc aatggataaa
     3421 gaatatggat gaatgcggca ttgcccgcac tgttgtgcac acccaggcat ccggtgcaat
     3481 atttgattcc atttataact tgtattcccc ctatggtgac aggtttcagc tctggtgcgg
     3541 atttgactac accggctatg atgagtcggg atggggacct gctgcaatag cggaacttga
     3601 aagatgtcac cggctcggag ccaggggcgt cggagaggaa ggtgacaagg gtatgggtct
     3661 gaccttcaac agacccgtgg cagccagggg tatgcatttt gatgacccga gaatgaaacc
     3721 cctgtttgaa aaatgtgccg aactgaacat gccggtcagc atacatgttg cagatcctat
     3781 ctggatgtat gaaaaaatgg attcgacaaa tgacggtctg atgaacgggt ataaatggag
     3841 gatcaacacc gaccggcccg gtattatcgg atttgatgcc ctgatacaga cccttgagaa
     3901 tgctgtaagg gataatccca aaacgacttt tattgcctgc cattttgcca atcttaatca
     3961 cgatcccgac aggctggggc agcttctgga aaaatatccc aatctctatg ccgacatatc
     4021 ggcacgcttt gcagagtcag cccccatacc gcgctatatg aaggcctttt atgaaaaatt
     4081 tcaggacagg ctcctttatg gtaccgactg gggttacgaa actgagatgt acaggaaaac
     4141 gttcagaata cttgaatcaa acgatgaaca tatttacgaa atagatcatt ttaactatca
     4201 ctgggctctc agcggattcg gactgcctcc cgggattctt aagaaggttt attatgataa
     4261 tgctgccgga ctgtttggtg attaagggcg gcatcaggga ctgatcactt caatatcatt
     4321 cagcaaggct gttttaagtc ttatttcatt ctgaagcagt tccagtattt gtttgtttgc
     4381 aaaatgctga tgtaccacca gcccgattgt ccgcgaaggg gcaggctgca caaaacggac
     4441 gagatgtctg cggtcttctt cccggaaatt acggacagcc agactcggca gcagggtctg
     4501 acccttatat gttttcacaa aacggaccag gctgtcaata gaacctgttt tgaaatgaat
     4561 ggaagaggga gatttgatat aggaattgcc cgaactgcag atttccagaa tctgatcact
     4621 gaggcagtga ccctcttcca gcagccagaa attatcccag ctcatgtctc tcagggaaca
     4681 tcctcctgaa tgaatgcctg aagtgtcata cagcacaaat ggttccctgt acacgggata
     4741 ctcaataagc tctttatcat tcagaggtgt tgaaatgatt ccggtatcaa gctcccgcga
     4801 ctttatcagg cttacaattt cgtcagttgt actttctttt atttccaggc tgagaccggg
     4861 atatttcacc gaaaaatcat acaggatcag tggcagcaga tagggtgcaa cagtggcaat
     4921 acagccaatt ttcagaatac ccgtgacttc gcctgtcatt ttttttacca gttcatttaa
     4981 ctgggttatt tcaaaattga tgaccctcag ctgtgctata atttcctgcc cttccctggt
     5041 aatgtcaacc ggtttcttct tcctgttgaa aatgataact cccagttcgt cctccagctt
     5101 tgatatcatt gtgcttaagg tggattgcga aataaagcat ttttcagcag ctgtttcgaa
     5161 atgccggtgt tcagccacgg caagtacgta ttgaaattgc tggatgttca tctattttat
     5221 caatattgtt attcaaaaaa tcatttttgt aaaacaagta tagtgattaa ttttgacaaa
     5281 actaataatt acaggatcat ggctgagaaa aagaaaaaac tgacatccac atcaggcagg
     5341 ccgattgcgg acaatcagga tgtaaaaact gccggtaaaa ggggaccaat gttaatggag
     5401 gattattggt tcctggagaa gctctctcat tttgaccgtg aggtgattcc ggagcggaga
     5461 atgcatgcca agggatcggg tgcatttgga aaatttactg taactcatga tattacgaag
     5521 tacacaaagg ctaaactgtt ttcggaaata ggaaaggaaa cagaagtatt tgtccgtttc
     5581 tccactgttg ccggagaaag gggggctgct gatgccgaac gtgatatacg tggatttgca
     5641 ataaaattct atactgaaga aggcaactgg gatctggccg gaaacaacac acctgttttc
     5701 tttctgaggg atccctacag gtttcccgat ctgaacaagg ctgtgaaaag ggatccgaaa
     5761 acgaatctca gaagtgccaa tcataactgg gatttctgga ctctgttgcc ggaatctttg
     5821 catcaggtca cgattacaat gagtgacagg ggtatcccga aatcctaccg tcacatgaac
     5881 ggctacggca gccatacatt cagtctgata aacgcggata acgagaggat atgggttaag
     5941 ttccacttta aaacggaaca gggaattgaa aacctcacca atgaggaagc tgccgcaata
     6001 attgcaaagg accgggaaag ccatcagaga gatttgttca atgcaattga gcggggtgat
     6061 tatccgagat ggcgtatgaa aatccagtta atgacggaag aacaggcaga gaaatctccc
     6121 ttcaatccct ttgacctgac caaggtatgg ccgcatgccg attaccccct gattgatgtc
     6181 ggggtactgg aattaaacag gaatcccgag aactactttg cagacgtgga acaggcagcc
     6241 tttaatcctg cggatgttgt tccgggaata ggattttcgc cggacaggat gcttcaggca
     6301 cgtttgtttt catacggtga tgcacaacgt tatcgcctgg gggtgaatta ctaccagatt
     6361 cccgtcaaca aaccgaaata tcccttccat aattatcaca gggacggggc aatgcgtgtg
     6421 gacggcaatg aaggctccac catccattat ttcccgaaca gttacgggca atgggaggaa
     6481 agccgggagt acagcgagcc gcccctcaaa ctgtcgggcg acgcagatca ttacaatttc
     6541 agggaagatg ataatgacta ctacagccag gctggcaatc ttttcaggct gatgtctcct
     6601 gaagagcaga agcgcctgtt ctgcaatacc gctgcggaag tgggcggagc cgaaaagttt
     6661 attcaggaaa ggcatatagt aaactgttac aaggctgatc ccgcttatgg aaaaggagtg
     6721 gcggatgcac tcgggattga tataaataca gtgaagattg aataggctgt atgccgggat
     6781 atttccatac attcagtaat attggtaaat gatttttata caaggtaaaa actgacaata
     6841 aaaaacttca ggaatttaat ctgaaatgta aggaaccggg atcccggaat tgaaaagccg
     6901 ggttaccggt ttttttattc ccggacaaaa atccgtgaat ggttgttaaa attgttgcct
     6961 gagattttga atacaattat tcggattccc ggattaaacg ctgtccgtgc aaaccggccg
     7021 gtatgcagac gggtagcagt gcttgtcaat gttgaaggat acttatattt ttgactttta
     7081 aaattgtata aaaaagataa gagatgagtt ttggtaaacg tttaccgatt tattgccgga
     7141 attatttccg gacttgtttc actgcccttt ccggagcccg ccggctcact gtaatattgc
     7201 tgctgtccgt cacggcagtt gcaaaggctg taaggccgga atctgtttac actcagcgcc
     7261 cggatgatcc ggaagcattc tatttcacac cggaaaacta cggtttcaaa gccggcggaa
     7321 aaagcgatgt gtcggaagcc ctgcagaggg caataaacca ggtaaaggca gaaaagaatt
     7381 ttggaattct gtttattcct gaaggaaaat acaggatcag cagaaccata tatattccgg
     7441 gggctgtaag actgataggt tatggcagta agcggcctga gataattctg acaaggaatt
     7501 ccccggactt tcagaaggag gctgaggatg acaagggaag ggctgcctat atgttctggt
     7561 ttaccggtgg tccggtttct gagggccgga aaccgcagga tgccggggca ggaacctttt
     7621 acagtgccat gtcgaacata gacctccgga tagaggacgg gaatccctat gcggttgcac
     7681 tcagaacaca ttatgcccag cacagctttg tcagtcatat gaatatatat gccggaagcg
     7741 gcaaggccgg gctttttgat gccggcaatg aaatggaaaa tgtggctttt tacggagggg
     7801 attacggaat atatacaacc aaagcctctc ccggatggcc cgtaatgatg gtggattcat
     7861 attttgaggg acagaggaag gcggcacttc atacacagga atcgggactg gctattgtaa
     7921 acatgcatgt gcgtaatgtg ccctgtgtta ttgagattga acccaattat gctgacaggc
     7981 tgtttattga ggacagccgt tttgagaatg tcagagggcc ggccctggtg atcagcaatg
     8041 agaacaacag taataatcag atcaccctga ggaatgtgga ttgcaggaac gtgccccttc
     8101 ttgcccggtt cagaaggagt gataccgaaa ctcctgtaaa tcacaggatg tacaggataa
     8161 ataattactg gcatggtctg cagatggatg atatgctgga tcagccccgt tacaggaccg
     8221 ttgcagatat tatccccctg gaaagttggt cgtcagaaat cagctcagtg actgtccctt
     8281 cccttccgcc ggtgtcatcg tgggtgaata ttcttgagct gggagcctgc ggggacggga
     8341 aaacggataa tacccggatc attcaggatg cgattgacat gtatgacaat atttacatac
     8401 cgcagggatg gtatctggtc agcaataccc tcaggatgaa gctaaatacc tgtctgatcg
     8461 gccttcatcc gatgggaaca cagctgatga taaaggaaag cacagctgcc ttcagcggtt
     8521 ttggtccgcc tgcagcaatt cttgaatcat cacggggcgg cagaaacatt ctcaatggta
     8581 tcggaatcaa taccaataca tttaattacc gggctgttgg ccttaaatgg atggccggtc
     8641 cggaatcgct ggtgaacgat gtgaaattta ccggaggaca cggaagtatg cagaaacccg
     8701 gagagcctgc ttcacgaacg gtttacacgt cccagggaat ggatccctca tgggacaacc
     8761 agtactggag cttatgggtc accggcaacg gcggcgggat tttcaaggat atatggacag
     8821 cttcaaccca tgccacaaac ggtctgtatg tcagtaatac atcttctccc ggaaggattt
     8881 atgccatgtc ggtagagcat catgtgagga atgaagtgag aatgaaaaat gtgtcgaact
     8941 ggaagattta tgcattgcag cttgaggagg aaagccgtga gagtactgaa tgccagccca
     9001 tggaactgga gaactgccgt gatcttacat ttgccaatct ctacatgttc agggttatca
     9061 ggattaaccg tccctatcat aattccgtcc ggatctggaa ttgtgaaaac gtggagatgc
     9121 ttaacctgca taactattcc cagatcaaat ataccacgga tatacctgtt tatgacatca
     9181 accgggatat tgaagtcagg ccctgggaac tgcagagact ggtaatcagc ggcagtgaaa
     9241 aaagaccggt cccgttgcag gcagtcccgg gtgttgcaga gaaacttgca gggggatttg
     9301 agtttattga gggtattacc cgtgacagca ggggcaacat atatttcagc gaaaaccgga
     9361 tgcgtcgtat ttaccggtgg tctgcagaaa ataatacatt atcgatggtt gctgatttcc
     9421 catggaaacc cgtgagtctc ggatgcgact cactggataa tctgcttgtt gttttcaggt
     9481 atgatccgca gcccggacat atgatagacg ggaaacccga gtcatattat tttccggagc
     9541 ttccgcagga agaccggggc ggcacatcct tcagcggctg gggcaattcg ggtttcgcca
     9601 tacgggtcta ttccattgat cctgacaatc ctgaggaaac aatcagtctt cttcccctgg
     9661 taccaatgaa gtccgtcgcc agtgttcaca aagcgctcta tgtatcaaat agatggcgtg
     9721 attttcatga tttcaacagc atatccctca gggttccccc ggattgtttc cttgcacctg
     9781 acaagaggac gataattccc aggcattatg atctggccag aggctcctct cttcttgagg
     9841 catttcccgg aaaacccttt tacacttcgg atgaatatga ccgcaggatg gtcaggttcg
     9901 atgtggcagg ggacggcaca ctttccaatc tgcattattt taccgaatgg ggagaattcg
     9961 gatcggcaac cgattccgaa ggaaatctat atgttgctga tggtaatata cttatctttg
    10021 ataaaaccgg caggatgcag ggaacgattc aggttcccga gcggccttca acgatacagt
    10081 tcggaggcag gaacggagac cagcttttca taaccggccg gtccgggttt ttccgggtaa
    10141 taatggaaaa accggattga aagattattc atcaacagcg ggcctgtttt ccttcatgac
    10201 attccatact tccagcggaa ggtcggtttc tataatatcc ggattactgg cctatttttc
    10261 cttcattaca ttccatactt ccagcggaag gtcggtttct ataatatccg gattactggc
    10321 cagttcctgc agatagcctt tcctgcgttc ctctctttcc tgcagcctgt catgcgtggg
    10381 agcaaccgaa atcatgcatc ttactcccct ggaatgcagg aggcggacca actcagcgtt
    10441 ttccgaggta atcgccgggc ctacgtaggc aatcatcttc ttccagggaa cacctgaaga
    10501 ggcaatattc tcatattcct tcattgtacg gacaaatgcc gaaagcatcg ccttcggcag
    10561 attttcatgg tagtattttg cctgggctcc gttatgaacg gtaagcatca catggccgga
    10621 tgccctgagt ctttttacaa gatcgacgat catttccggg ggtacgtctt tccggtcgag
    10681 attgacaacc gttctgcccc tgctccatac aatcacctct tcaagggtag gaattgaaaa
    10741 ggctgtcggg ttaccggcaa aatccctgag ccggacatcc ctgagctgct cataggtata
    10801 atcattaaga ttgccttttg cagttgtggt tctgtcaagg gtggcgtcat gcatgagtac
    10861 gatcacgctg tccctggtaa gccgggggtc gatttcgaaa aaggcaggca tctggcggag
    10921 gacatttctg aagccttcca ggctgttttc aggataaccc ttttcccttc ctccgcggtg
    10981 gccgctgata ataaccggag caccaggacg gtagcgaaaa aatttcatgg aactgccctt
    11041 tttcagtata tgaaaaccct ccgggtctgt tgcctgtgca tgaaccggcc ggagcggaac
    11101 ggctgctgta atgaacagaa aagtgatcag taacaggttt ttgtatttac ttttaagttt
    11161 catatccctt tgtgtgtttg tattatgaag gtttcttgat cttttgtgtg gttgtgacat
    11221 gaaggtttta tcagattgtt tttataaggt tttttataaa actatgaacg tttttctcaa
    11281 tacctggatc acagcatctg aagaataaaa ataaaaaaat agtttgacag tagtcctgtg
    11341 gaaatttaaa acattaaatt gaaagcggat actttgcgga tttaaaatcg gaatactgag
    11401 aattcaacaa cacatccggt gatttaacta ttaaccgggc tgcaaataat tatagaaaat
    11461 gaaaaaacaa aagactgtaa atcttttcct gttgcttctg agcctgttgc ttgcctccct
    11521 gtcgtgcagg caaggcagtt cagatacagg aacagcaaat attgacaacg ggttaataag
    11581 tattgctttc aatgaccgga acgggtcgct ggtgaatttc agcgatattt ccggcaagca
    11641 tgagtggatt gacgacgatg tggctgccgg ttctccctgg gaactttatt ttaccgaagc
    11701 gtcaggcatt gaaccaataa atgcagccga tgccggtaaa ttccgttttt ccaaaccgga
    11761 ttcccgtaca ctgatactga aatggaagag attcggaaat cctgggataa ggaatctgga
    11821 agttgaggcg agagtcagtc ttgacagtga caaggccttg tcatactgga atattagtgt
    11881 ccggaatcct gacaggaaac agatcaggca gcttaactat ccgaagctgg ccggtctgag
    11941 ggaggcaggc gaggaatatc ttgcagttcc ccagtggatg ggacagatca caaaaaaccc
    12001 cagggagagc ctggctccgg gagtaagtgc tgccggcaga tatgaatgga catatcccgg
    12061 gctctcgctt cagtgccttg cactctatag tcccgggaga agcggattat atattgcatg
    12121 taacgataca ctggcttaca tcaaaaattt ttccttcact ttggacagtc tcaacacctt
    12181 gacttacagg atgcacaatt ttccatcaat tgatccctca tcagcatcat atacaattcc
    12241 ttattcagct gtggtcgggt cattcagggg tgactggatc actgctgcgg aaatatacag
    12301 ggaatgggcc tcggggcagc gatgggcgag ggaaagcagg tttctgaaag gactgacacc
    12361 ggagtggctt gagaagacgg cactttgggt atggaacagg agcaaatcgg ataatgtgct
    12421 tgtaccagct gcagatatca ggcagcggct gggattgccg gtaagtgtat tctggcactg
    12481 gtggcacgga tgttcctatg acgatggttt tcccgaatat cttcctccga gagaagggag
    12541 aaaatcattc ttgtcggcta tggacgaggc gcataaaaag gggataaggt caattgtgta
    12601 tatgaatcag cgcctctggg gtaccgaaac cgaaagctgg gagaaagaaa atgcagctga
    12661 gtacagcgta aagaattctg atgggagcat gaatacccat gtttacaata ttttttccgg
    12721 caagggcact gcaagcatgt gcctgggaac agctttctgg aaggacaggt atgcatcgtt
    12781 gtgcgacagt gttgtcaaca gctatacggc agatggtgtt tatatggatc aggcctgcct
    12841 tgctctgcca tgctatgatc cgggccatga tcacccgacg ggtccgggaa attactgggt
    12901 ggagaatttt ggcaggctca cggaccagat aaggtcaaag gtagcgggcg aaaagcagat
    12961 gatccttgcg ggtgaaggct gcggagaggc ctggcttccg catcttgatc tcatgcttac
    13021 cctggctgtc agcaaggaaa ggtatgcagg gctgaatcaa tgggaaacaa ttcccttttt
    13081 ccaggcggtt tatcatcagt attccataac ttacggcaat tattcctcgc ttcttgttcc
    13141 tccctacgat gagctctggc cggaagaata tgcccctgaa gatcctcttg aaatgctcga
    13201 caggaagttc agcaggcagt ttctgatgga acaggcacgg tcctttgtct ggggcatgca
    13261 gcctaccata gcgaattatc atgccaggct tgcaagccac aggaaggaag agattgattt
    13321 tctcctccgt ctctcccgta tcagaaacaa taatctgaag tacctgttac acggcaaatt
    13381 tatgaggagt ccggatatcg gttctccgtc agaggaaatt gatatatcca ggctttccat
    13441 ctatgcggga aagaccggga acaccgtcag tgctttccgc ggaacgttcc cgctggttta
    13501 ttccggtgcc tggaaggcgg atgacgggga tctgggaata gcactggcaa gcacaggcga
    13561 caggcccgtg agcctcagct ttggttttga tgcaggtgaa tacggtctgc cggccgcagg
    13621 taggatattt ctagctgatg acaggggaag gagggaactg actgcctata cggacagcag
    13681 ggcagaaata tccctcaccc tggctcccgc ggctgtatgt gttctggaag tcattccgga
    13741 tcacggaagc agttcgggaa atgaaatgca gtgacctggt ccggctggtc taatcaatta
    13801 tcctggcatt gcggaatgca gtctgaaggt cgctgagctt catgtttgtg accctgatat
    13861 acctgcgctt acccgcagga agtacggttc cggcatcgtt ttcagctgcc tgtacccgta
    13921 ttgtgcttcc gaggttttcc ataagggtca gatcagcggc ccgcagtggc agttccagtt
    13981 tcctgtcgcc ggatatatgc cagtaacatg cataaagatc cctgtttctc cggaaggtaa
    14041 aagcgatcac ctccctgctg ccgccggcaa catcttctat ctgatcgcag gccaccagtt
    14101 cgaaatcgtt tttttcattc agcagcagca taaattcctg atccggatcc tgcagcatct
    14161 tcttctggtc ttccgtcagc cacttccttg cccgtacctc ttcccacctt ctgaaaacct
    14221 caaagttgtc ttccgttctc ggatgtcttg caagggttgc gggataggca tgaatggata
    14281 cggggcagtc ccatgcagct gccttgcttg tcacaaattc aagctgatca ggctgtgttc
    14341 cgatggtctt ctcatccgga agccagtatc ccagccagcc gaaattcagc cgtgttaaat
    14401 tatcccgcat cctgggtgct tcccggaaag gatgttttat ggtttcttcc ttgagctttt
    14461 caggggggaa aacgtcaaag gcgtttcctc ctgaaagcat atgccagctg aaatgggtct
    14521 ttgccgcacc ttccgcaaat atgggttcgg gtttcagttt gctgaatacc ctccactgag
    14581 cgttggcaac atgaaaccag aaaggaggat tgactccctc tgatccgtcg aagtaaataa
    14641 attcaaaccc cgcatcccac agatctgcta tataaccggc aatctcatcc tgcagaccgg
    14701 aatactggtc gatatagaca cttgttgccc cgaactcgct gacatccagc agtccgaaca
    14761 tatagcccgc cggatgcgcc ccggtcccgg tattgtcaat ccctctcacg caaccccgga
    14821 agctgtacgg gggagttgtg gtataggtct cgtacgaaac aagttctccg cctattttca
    14881 gtactttcct gtttcccgcc atggttgagt tctgaggatt ctgttccacg taaatgactg
    14941 tatccgtttt gcccagtggt tttgcaaggg tgaaaaccct gagcaggttc agcctgtgat
    15001 cagccacggg agtcacgtac ctgctgtctc ttccgatatg gctgtgaagg aaatggcatc
    15061 cgggagtgat gccggcatcc tttatctttt gaagcattgc tttcagatcg tccttgccgt
    15121 tggggtactc aggcctgtat acatcatagt ttccgatcag cctgtagcct ctgctttcga
    15181 ggaaggccgg atagtaaatg ttcatcagtc tgaatccccc catccttgca tattttatgt
    15241 gttcatcggc attgaggggc gtaatgtttg acgtccagta atatgaagca ttatagagat
    15301 tacttcttct gctgtcagca ccagcgggca ggccgtaatc cttttccaca acggctattt
    15361 tgtcaagcag ctgatcacgg gagcatgtca gaagaacggc tgtgacatca gtcagtttaa
    15421 ccttttcatc cactcctgcc tgaaggatgc gatagccatc gccttcttcc gaatcagcat
    15481 tggcacaagg ttctgcagct aacacgcaca cagccacttt ttcgttccat atcacattca
    15541 gccagtcgcc ccagtgaccg aggttcctga cgggaaggcg aagaaaccac atttcggata
    15601 tggggggttc tgtcatcgtt atgccatatg gctccgtgag gttaaactcc tcaagggtaa
    15661 aagctatata atcagcagtt acccggaatg ataccgtagc ttcccaggga ataagctcgt
    15721 atcctattat cagcctgtcg ccctcctgcc ttacactgtt tgatctgaag gtggtctttt
    15781 tgtttggata tgccagtttg acctcattct ggtagggacg cggttcggtg attgtactga
    15841 agggtatctt cccgccctgt atcaggcatt cggttttgcc gggcctgtaa acaagacttt
    15901 ttgtggtgcc gtcagctgag ataaccagcc tggcctgatc gttctcaatt atgatatcgc
    15961 cgtcgctgac tgaatttttt tgattacagg ctgtagtcag aacaaatcct gcaatcagga
    16021 ataataaact gagctgtttc atgatatgtg gttccggttt taatatgaca gggttttaat
    16081 atgacaaggt tttgatgtta caaggatttt atgttacagg tttttgatgt tactgggttt
    16141 tgatgttacg aagttttgat gttactgggt tttgatgttg cagggttttg atgttactgg
    16201 gttttaatgt tgcagggttt ttaatgtacg attattcaaa tataggcaaa caacagcttc
    16261 atgccatcat tatcacagct taacttaaat tgtaaatcct tacgttcccg gattattctt
    16321 agtctccgaa cctgaaatag attgtgcccc cgagaggttt tgcctcgcgg tagctgtttt
    16381 tttgtgaccc ggggcccagt gattcgggtg gattgaattt tgttcctatc ggagatatgc
    16441 tgttgagaag ggaaatattt ccttccggga attccggggt aacccagcct gatgcatgtc
    16501 cgggattttg cggagtgaaa agatgaagat aaatatcatt tgttgaactg actattgtta
    16561 aagggagatc agcggtaccg attctcactg cataaaaatt ggaataataa cccttgaatt
    16621 ccggataatc ccaggattca ccagttacag tattgttgta ttccttgtgg tacacaccga
    16681 acaaagggcc tttaaggcgg tttttccata cccggtaggg accgtttgcc atcagaaagg
    16741 catcctttac cgtttcttcg ctgaaggaga aggttattcc cagaagatcc tgcagtccgg
    16801 ccgggcggta gccatagtcc agcctgagcc atccgcccgg cagcattgtc catttcacgc
    16861 tgcctccgga ccttcccgga tagagagcct caaccacata cccgtcaggg gattcataat
    16921 gccgtacctc agagggaaca gaacttattc cgacaaaacg gggtccgtca gtgaagttac
    16981 tgcttctgcc atctctcgaa gccccggcta ttaatccgtt tttaaggttt atcagcactt
    17041 cggtgcctcc tgaagaaaga gagatgtgat catctttcac agcagacaca actttttcat
    17101 ctgtttttct tacaattctt tcagccatct ccgacgggcc ggtaatattg aatgaccaaa
    17161 tgaccagctg tttatcagtc ttccggaagg atcttcggca tctatgcgga tagcatcata
    17221 gtcattccat ccggcttgca gactgagctg cacctggtcg cgctgccccg gccggagcgg
    17281 aggtatgtct atttgttgct tttctgactg agtgacagat acgggaaaag aatccgaaaa
    17341 ttttattaag taataggata acctgcattt gtcaagtgaa gtgtagagaa agcggttttc
    17401 aattttcagt ctgccgtcaa attctggtgt gattattttt ctttccagcc agagcggaga
    17461 ccagacatcc cttactgtaa accagcttcc ctctttctcg cggtaaggcc cgacaatgcc
    17521 gtcaggagcc tgattgccag cggtatcaat ggaatccttc ctgtcggtcc tcacaacccc
    17581 ctcatcagcg aacagccaca ggaagcctcc agcggagagt ggatttgaaa gcatgagatt
    17641 ccagtaatca tccaggccgg caccgcttcc cccgtcataa caaccgtgaa gccattccgt
    17701 cggaaagaaa atatcacgtc cgttaaaaaa tgttgaaaca ccgtaattat atggaatata
    17761 atgttttgtg tccattccat taaagttgga tatgggttct ataaccaggc gtttctgcgg
    17821 atcgtatttt gcaaactctt ccctcagttc agggttgaaa cccctttcat tcccgttggc
    17881 ccaaagaatg atgcaggggt ggttaacatc cctttcaaca agttccctca ccagtttcct
    17941 gcctggtccg gtatcataat aggcctgcca tccggacaac tcattaataa caaaaagccc
    18001 gagtgaatca catacttcca gaaaatgctt gtcgggaggg tagtgagtca tccttacggc
    18061 atttacattc atgtctttca tcagattgac atccaggata cttatttttt tgcttgtcgt
    18121 tttgccggag gaaggccagg aattatggcg gcatattccc cggaacatga ctctgacacc
    18181 attcacataa attccgtcat tctgtcttac ctcaactgtt ctgaatccga tacgctcttc
    18241 tgtttcatac agtattgcat ccttgtcctt cagtctgaaa accgccctgt ataggtcggg
    18301 cgattccgga gtccactgtt tcggcttatc agcctttccc ctgatcagga cacgggtctg
    18361 accttttttc actccggtgg taaaagggcc tgcaacagct ctgccatcgg gtgtcataat
    18421 cattgcttca atgcttcttg tcccggaaca gcccttaaga gttaattctg cagtgaaatc
    18481 tccgtcagct tcagcattca ctgcaacatg ttcaatgaat tcatagggta ctattttaag
    18541 gtaaacagga cggtgaatgc ctccgaatat ccagtaatcc gcttcacgtt ctgccctgtt
    18601 gatactttca tcagctgact ttttgctgac agttacctca agcaggttgc tgccacgggg
    18661 ttttatcagg gaggtgattt cataggagaa tctgtaaaat gcaccctgat ggacggttcc
    18721 tgccggcttt ccgtttaccc ggacctccgt gtcagtcatg gatccttcaa atacaaggaa
    18781 tatccttctt ttacctgcag cagccgggac agtaaactca tgtctgtaca ggcccttttc
    18841 gtcagcctgt tgtttatcat ttccgtaatt gtatgttccg aaaccctgca gctcccagtt
    18901 tgagggtacg ggtatttttg tccatttacc gctgttttgt cctccggtac agaaaaaatc
    18961 ccatagtacc gtattttcat tgtctgttcc tgagagatag acaatctctg ccttttgtcc
    19021 gtttgctgct gcaataagaa gaattgcagt aagcaggcct gtaatttttt tcatgttgtt
    19081 ttccgcttta gctataaaga taccctgtct ggtatgatta tctaaagata tcatattatg
    19141 atgaggagag acaatgaaat gcctgcaaaa tcagtgaagc agccatcctg ccaaacctgc
    19201 caggagaatc agcgcaatag gatgcatctt ggtttttact gacagaagaa atgctgctat
    19261 aa
//
LOCUS       DAOSPX010000023        19244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3017_length_19244_cov_5.093491, whole genome shotgun sequence.
ACCESSION   DAOSPX010000023 DAOSPX010000000
VERSION     DAOSPX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3017_length_19244_cov_5.093491"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLZ75_02260"
     CDS             complement(<1..698)
                     /locus_tag="PLZ75_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOS71253.1"
                     /translation="MSVLVYTENQDGRFKKHIFELVSYASETAKMLNCSVTALSTGKV
                     EESELMKLGNYGAGRIISVENSLPSGLDNRAYSYVVSEVAGKTGATVIILSDNNTGKA
                     IAPGLSVRLKAAVGSGVSRLPLSTEPFVVYKRTFSGNAYAHVVLKTPVRIITLARNSF
                     SSIESAGNPSIEKMEIPVDGALVTTTIKEVIRQSGRMQLSDADIVVSGGRGMKSPDNW
                     APLLELAESLGAATA"
     gene            complement(726..1475)
                     /locus_tag="PLZ75_02265"
     CDS             complement(726..1475)
                     /locus_tag="PLZ75_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOS71254.1"
                     /translation="MKILVCISNVPDTTTKIRFTEGNTSVDTSGIQWVINPWDELSLT
                     RALELRDDPSSGVKSVTVIHSGPASAEPVIRKALAIGADDAVRIDSPSGDAYSVAAGI
                     AEVVRKEQYDIILCGVESSDYNGSMVGGMIAEFLEIPSVSGISELKAEGGNVTITRET
                     DGGKEIVSVPLPFTGIVQKGIAKEPRIAAMRGIMMARSKQIRVYEPGPEEALTGIEVF
                     EKPPARGACKFIDPDNPEQLIDLLQNEAKVI"
     gene            1909..2832
                     /locus_tag="PLZ75_02270"
     CDS             1909..2832
                     /locus_tag="PLZ75_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOS71255.1"
                     /translation="MRDFIFRYGTVTLSMVFWAFSFIWFKVANETYRPLTIVFIRLVI
                     SVILLNIFISLSRSRIRIKREDRKLFILLAVFEPFFYFLGESFGLTYVNATTGSVIIS
                     TIPVFAATGAWVLFGEKLKPVNYTGIIVSFIGILVFLLNRDGSLSFNIRGILLMTFAV
                     FSAVGYNLTLSRLVGSYDPISIVNIQNMIGALLFLPLFLISDLDHFISTPFQAKALMP
                     VAKLAVFASCGAFILFAYAVRQMGVTKANVFTNCIPLFTAVFSFFILGDILTLQNISG
                     MAIVITGLFMSQYNGRSGKSGEVKVLTGKTA"
     gene            2910..3944
                     /gene="porQ"
                     /locus_tag="PLZ75_02275"
     CDS             2910..3944
                     /gene="porQ"
                     /locus_tag="PLZ75_02275"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOS71256.1"
                     /translation="MGKRILYIFLTGILAINSGAQTGGDNVYEFLNLTHSGLVSSLGG
                     TNVSIYADNLNLVYHNPSLLSSEMDKSLAMSFVSYFAGINYGQVMYALSVPRAGDFAA
                     GISYLNYGSFTEADPSGNITGKFYASEYAFSLAWSREIHEGFNAGINLKPVLSHLERY
                     SSFGLAMDIGGSYHSESGHFSAGLAIRNLGVQITTYAGEKRLSPPFEIQAGVSRKLAH
                     SPFRFSLTLRHLQKFKLTHLYDDPEESSQDSGFFENLMRHAIFGVEMIPHRNFYLAAG
                     YNYQRRRELKTGSGSSAAGFSWGFGIKTSAMNIEFGRATYHLAGSSNHVSLILKTDRI
                     YKKLWTKESQ"
     gene            3923..4618
                     /gene="cmk"
                     /locus_tag="PLZ75_02280"
     CDS             3923..4618
                     /gene="cmk"
                     /locus_tag="PLZ75_02280"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOS71257.1"
                     /translation="MDKRITIAIDGYSSSGKSTFAREIAKELGYIYIDSGAMYRAATL
                     YCIRKGLAGRDVIDIQGIAEAMKEIRIEFVYNPDSGESETYLNSENVEKEIRGLDVSA
                     HVSAISKISEVRTRLVELQKQMGANRGIVMDGRDIGTTVFPDADLKIFMTAPVDIRAK
                     RRYEELKNKGIDASFEEIRNNIVARDITDENRDISPLRRAGDAVILDNSRMSIAGQME
                     WVRKLIEEKLHDR"
     gene            4608..5483
                     /locus_tag="PLZ75_02285"
     CDS             4608..5483
                     /locus_tag="PLZ75_02285"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOS71258.1"
                     /translation="MIVETDNFSGFCFGVRNAVEIAEKALRAGEEIYSLGLIVHNEAE
                     INRLVALGLKMISHKELDSLKNSKLLIRAHGEPPETYRTAEKNNITVIDATCPIVKKL
                     QARIRETWLKSRENGGQIVIYGKPGHAEVIGLLGHINNDGIIVSGPGDLSGIDFSRPV
                     MLFSQTTMSVKEYNRIAELIRESMAQSGITDTDSHLKVHNTICAQVSGRESQLRSFAQ
                     KHDIIIFVSGKDSSNGKLLYSVCKSANPDSWFVSSPGEIERSWFEGKKSAGICGATST
                     PKWLIEEIRNLIISF"
     gene            5551..6669
                     /locus_tag="PLZ75_02290"
     CDS             5551..6669
                     /locus_tag="PLZ75_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS71259.1"
                     /translation="MEKSKVYFTDLRIKPGLNLLKKLNNLIIKAGIKQIEFEKKIVAV
                     KIHFGEPGNLAYLRPNFAAHIVKFLKAEKALPFLTDCNTLYHGKRANAPEHLEAAFEH
                     GYNPLVTGCPVIIADGIRGGEYREVELNLELTRSAKIGSAIADSDIIISMNHFKGHEL
                     TGFGGALKNLGMGCASVGGKLYLHSGTSPVITEENCTGCKMCERYCAHDAIKVESDKI
                     ARIDYEKCVGCGQCVAVCQYNAAGFVLNHSSELACRRISEYALAVVRDKPSFHINFIL
                     DVSPDCDCWDANDVPIVPDIGMAASFDPVALDKASADMVTSSPGLRGSKILGKRSDGD
                     LSGMDKFRMTHPDTHWQSGIEHAVKIGLGNADYELIHV"
     gene            6725..7573
                     /locus_tag="PLZ75_02295"
     CDS             6725..7573
                     /locus_tag="PLZ75_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOS71260.1"
                     /translation="MNLKVKTARLSIISNTLLIVLKLGAGLASGSVSIISEAIHSFMD
                     LLAAVIAFFSVKISDNPPDARHPYGHGKVENVSGVIEALLILAAAGFIIIEAVKKLTG
                     KPFDLEFIWAGIIVMIISSIVNFIVSRRLYRVASETGSIALEADALHLKTDVYTSLGV
                     AAGLGLIMLTGIKWLDPLVAIMVAGLIIYEAFNLLRKAFWPLIDTAWTDEEIRLLKKK
                     LEMMKVNYHGLRTRIAGNYRFIDINVVIPAEHSVADAHRFCDMIEDELSRNYENLTVN
                     IHVEPS"
     gene            complement(7575..8558)
                     /locus_tag="PLZ75_02300"
     CDS             complement(7575..8558)
                     /locus_tag="PLZ75_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS71261.1"
                     /translation="MKKRKKLWKADEVVHPFRVIVHKEISDQVNSWRFIILLFIIGLT
                     CFGSMYTALTNIGSAVQANDQEGNFLFLKLYTISDGTLPSFFVFISFLGPLLGISLGF
                     DTVNSEQTKGTLSRLMSQPIHRDYLINAKFVSALTVISVLFFALGFLFMGLGIIITGL
                     PPTPEEFWRVVFFILVSILYVAFWLNLAILFSVIFRQPATSALACIAVWLFLTVFYNL
                     IVNLIAKAIAPSAMASTHQVFRYQEIIMTLMKILPGELYSDATTTILMPSVRSLGPLT
                     MEQIVGAVPGPLPLGQSLLLVWPQLTGLIAATVFCFVLSYVTFMRREIRSR"
     gene            complement(8884..9546)
                     /locus_tag="PLZ75_02305"
                     /pseudo
     CDS             complement(8884..9546)
                     /locus_tag="PLZ75_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(9550..10701)
                     /locus_tag="PLZ75_02310"
     CDS             complement(9550..10701)
                     /locus_tag="PLZ75_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOS71262.1"
                     /translation="MLKYSKSFSLAALIIFGSFLSGITAGASADSVTLFTPNTRISVP
                     PGESITYSIDVINKSKGVRNMDISVPGIPRTWVYSLTSGSYKVGRIAVLPEQKQTLTL
                     KVDVPMKVNKGSYGFSVVAGGGASLPLVIVVTEQGTYETEFTTKQSNMQGHSTSTFTF
                     STELRNRTGETQSYAFRTNAPRGWNVIFKPNYNQATSVEIEPNSTSNVTIEIKPPVNI
                     QAGTYQIPVVAATNVTSANLDLEVVITGSFNIELTTPSGLLSTGITAGDSRRIELLVR
                     NTGSSGLDDVEMSFSAPVNWDVVFDPPKIDRIEAGSNSKVFATIKADKKAIAGDYVAN
                     LEAKTPEASSRATIRISVKTPMLLGWIGVAVIAVALGSVYFLFRKYGRR"
     gene            11042..12958
                     /locus_tag="PLZ75_02315"
     CDS             11042..12958
                     /locus_tag="PLZ75_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS71263.1"
                     /translation="MKRGKKRGVKAAKATEADEKQKRQRKAMKGNEDDKIKGNEENKD
                     KSTVSGLRELMKLYRFLKPYRWIFILGLFFLLISSAASLMFPKYLGNMVDIANRGNLA
                     GEISRTGLILLAIVITQAIFSYSRTRIFVYVTEKTLASIRQHIYNHLIKMPMAFFAER
                     RVGELNSRISSDVAILQHSLTETLADFMSQLLIITGGISLMMVTSFKLTLFMLAIVPP
                     MALFAFFSGRAIRRYSKKAQSHVAESNTIVEETLQGIQNVKAFTNELFEINRYRDKTD
                     EVARTGIKGGKYQAAVSFIILGFFIAMGLVIWRGALLIAKGEMEAGQLFSFVIYSGFI
                     AGNIAGMAGVYTRLQRTIGAAENLLLLMDEATERIDEQYIPDPDYMIHGQIDFDHVHF
                     RYPSRDEMTVLEDVSFSIKPGQEIAIVGPSGAGKSTIASLLLKFYEPDRGRILFDGRD
                     SREFPVTALRSQMAVVMQDVFLFGGTIRENIAYGKPGASDEEIIQAAVKANAWDFIKS
                     FPGKLDTVVGERGVQLSGGQRQRISIARAVLKDPIILLLDEATSSLDSESERLVQEAL
                     DLLLRGRTSLVIAHRLSTIMHADHICVLENGRIIEEGDHSSLLEKQGMYHAMWRQQTG
                     ERENSKMISRTEVI"
     gene            13121..13576
                     /locus_tag="PLZ75_02320"
     CDS             13121..13576
                     /locus_tag="PLZ75_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOS71264.1"
                     /translation="MARSQETFGKKEVRNRKEKKRKEKEQKRAQKRSEGKKASFNDMI
                     AYVDEFGIISSTPPDPGKKTEVDAQSIELTIRHNSPETDPAPGKKGVVSFFNKSKGFG
                     FIRDSDSGQRIFVHMNNLLEPVAENDEVSYEITRGTRGPSAINVRLLRE"
     gene            13678..14352
                     /locus_tag="PLZ75_02325"
     CDS             13678..14352
                     /locus_tag="PLZ75_02325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOS71265.1"
                     /translation="MKSEDNSRNMDPEPMFSVITASYNRGGLIERALKSLTGQSEKDW
                     EGIIVDDGSTDDTYSRIFPFLKADSRIRYFRKEHSGEALTKNYGIRMSSGRYITFLDS
                     DDEYHRDHLASRKKILLQNPGIRFLHGGVEILGNQYVPDRNDPSRPIHLKDCIIGGTF
                     VIERETMLSLEGFRNIKLGPDSDLFERALRRGIPMMKLDLPTYIYHHENPDSITNILF
                     GKIQDH"
     gene            complement(14346..15539)
                     /locus_tag="PLZ75_02330"
     CDS             complement(14346..15539)
                     /locus_tag="PLZ75_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOS71266.1"
                     /translation="MNTDKFLSLLDRLTATGKRIYFTGLIIALLVTSNEVIRQIYNNF
                     QIFSYGSLDFWNGINPYSDWNHLSILGKQLDVFIYGPLFSILFTPFTLIPGWAGVYLW
                     NIFTYTLFYFSVFTLPEKFSFNTKRFIFFFSSLLLFTTLLSVQFNPVVAALFLFSFTL
                     LEKNRGFWAVLLILVSGFTKVYGIFQIIMLLFYPRLWRNIFYAVLIGTFLFLLPLVRI
                     PLPELIDYYNSWIFLVTNHLEGFNKYSIYRPFLAVDGSIASITGFISLGVFAVIAIFT
                     LIRLKIFRESFILRAQYLGLLMSWVILFSAGSERHTYVVAAAGYAIWYSSIFPARPEK
                     ILLWINLLLLGVLPVDLLCPRMISDIILARLHLGVIVFSITWFIMVKKTFFSSGLQRE
                     LPAFQ"
     gene            complement(15717..16505)
                     /locus_tag="PLZ75_02335"
     CDS             complement(15717..16505)
                     /locus_tag="PLZ75_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS71267.1"
                     /translation="MDGSKYILFTIDLEEFDLPLEYGISLPSGRMMSVTNKGLSRIYD
                     LLEKHNIRATFFITSGYAEANAGMVRKIAEKHEISSHSHSHTIFHPGDPGKSKNILEE
                     ISRQKINGFRMPRMAKTDLNELKQYGYSYDASVNPTLVPFRYNMLSSPRTLFRDKATG
                     LPVVPVSVSPVIRFPLFWLSFKNLPFGIFAFLCKRTLDKDKYLHLYFHSWEFADIRHF
                     RIPFYIRRKDGDQLTNLMERLLLSLKRQGEFIGITEFLQIRKMI"
     gene            complement(16492..17211)
                     /locus_tag="PLZ75_02340"
     CDS             complement(16492..17211)
                     /locus_tag="PLZ75_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS71268.1"
                     /translation="MVTKRDLAIILPVYNPHQGWEGELLCALGKIQEIFSGVDYQITI
                     VNDGSTDGVDDIIRNGILPEYPDIKYLCYPRNMGKGFAIRHAMERSASDYYIYCDFDF
                     PFGTDVLRKIYEILAGGSTNLVMASRNFNTFIRRLPFIRRLVSLSLILINYCITGFRI
                     KDTQAGLKGLDNRARSIFLTTKTNSFVFDLEFALKCLKSGIKFGFVKVDLNPEIRFNN
                     FSSAVLRRELVNFLKIIVGRV"
     gene            17556..17945
                     /locus_tag="PLZ75_02345"
     CDS             17556..17945
                     /locus_tag="PLZ75_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71269.1"
                     /translation="MTGGLALNGQAVQQFYHNRIENAIDEISSTEVNSGAVVWKLYNH
                     TYIIKTPSVTIGFDIQRGIPGNENFAFSRKITDRLIIPGHENELNHSTDHREPCWLNY
                     TRLGNDPALPWIQMAPGEKYHYNSRMQ"
     gene            18033..18209
                     /locus_tag="PLZ75_02350"
     CDS             18033..18209
                     /locus_tag="PLZ75_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOS71270.1"
                     /translation="MEDIEICHCNGIMKSEIVKAIKEKGLKTVEEVQDETEAGTVCGG
                     CIPDIEDILSEING"
     gene            18218..18553
                     /locus_tag="PLZ75_02355"
     CDS             18218..18553
                     /locus_tag="PLZ75_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOS71271.1"
                     /translation="MGKNEFEKGKKFSFADSITYAEKSVVSKHVLKKETGNISLMSFD
                     KGEGLTEHTSPFDAVVHIVDGKADIVIDGVSNILETGDTVIMPANIPHSLSAVERFKM
                     VLIMIRSKQ"
     gene            18570..>19244
                     /locus_tag="PLZ75_02360"
     CDS             18570..>19244
                     /locus_tag="PLZ75_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HOS71272.1"
                     /translation="MVIGIDIGSTTTKVAAIMPDKEIIRIKTRAIDAITSATGAFGKL
                     IVENHFSLDSIKKINITGVGAARITDNLFGIKTNRVGEMTAIGTGGKYLAKKDRIIIS
                     NIGTGTAIIEVDGSKINHLGGTGVGGGTIIGLSKIMLGTTDFDTIMQYASKGSINNVD
                     LLIGDIADTNISFLDKEFTASNFGKMLDIAGKEDLAMGIFNLTYQVIAMVSVFAAKSK
                     NNDTVVV"
BASE COUNT         5614 a         4421 c         4371 g         4838 t
ORIGIN      
        1 gcggtcgctg ctcccaggct ttcggccagt tccagcagcg gggcccagtt atccggcgat
       61 ttcattcccc ttccgcccga taccacaatg tcggcatcag acagctgcat ccttcctgat
      121 tgtctgatca cctcctttat ggtagtcgtg acaagcgccc cgtcaaccgg gatttccatt
      181 ttctcaatgg aaggattgcc ggctgattca atagaggaaa acgagttccg ggcaagggtg
      241 atgatcctga ccggggtttt caggacgaca tgtgcatatg cattgccgga gaatgtcctt
      301 ttatagacaa cgaaaggctc cgttgatagc ggcaagcggc tcactccgga tccgacggca
      361 gccttaagcc tgacagacag gccgggtgca atggccttgc cggtgttgtt gtcggagaga
      421 atgatcactg tggcacctgt tttaccggca acttcggata caacatatga ataggccctg
      481 ttgtcaaggc ctgagggaag gctgttttcc accgatatta tccggccggc tccgtagttc
      541 cccagcttca tcaattcgga ttcctcaact tttccggttg acagggcagt aacagagcaa
      601 ttcagcattt ttgccgtttc cgatgcatag gagaccagtt caaaaatatg cttcttgaat
      661 ctcccgtcct ggttttcagt atatacaaga actgacatat atatttggat tatttatatt
      721 cccgtttaaa tgacttttgc ctcgttctga agcagatcaa tgagctgttc cggattatcg
      781 ggatcaataa atttacacgc tcccctggca gggggttttt caaagacttc tattccggta
      841 agagcttcct ccggcccggg ctcataaacc ctgatctgct tgctccgggc catcattatt
      901 ccccgcattg cagcaattct gggttccttc gctatgcctt tctgtacaat cccggtaaag
      961 ggcaaaggaa cgctcacaat ttcctttccg ccgtcagtct cccgtgtgat ggtcacattc
     1021 ccgccttctg cctttaactc cgaaatacct gacacggaag gtatttcaag gaattcagct
     1081 atcattcccc caaccatcga tccgttgtaa tcacttgact ccaccccgca caggataatg
     1141 tcatactgtt ccttgcggac aacttccgct attcctgccg ccacactgta tgcatcaccg
     1201 gagggtgaat caattctcac cgcatcatca gccccgatgg caagagcttt ccgtatgacg
     1261 ggctcagcag aggccggtcc cgaatggata actgtaacgg acttaacccc gctggacggg
     1321 tcatccctca gttcaagggc acgcgtcagg gacagctcat cccagggatt gataacccac
     1381 tggatgcctg aagtgtcaac tgaagtgttc ccctcggtaa atctgatttt tgtagtggta
     1441 tcgggaacat tgctgatgca aaccagaatt ttcattttat tcttctaata attatattat
     1501 taataaccaa tatattatat aaaatacgga ttccgtttat catgtcagag gcggcaaata
     1561 taataattta tgattcacat tcaaacctgc agaaaagtaa ttttaaaaaa caattcgaaa
     1621 gccagactaa tattaatcta ttgaattaca ttttattgta cagaaaatat atcaacggaa
     1681 tttcccctgg tcgaattgca gccggcttca gtccggtctg tcacggtttc gctgtgaaac
     1741 tgatgcagct ttgccgggag gagaactgat gaggctttgc cgggaggaga actgataagg
     1801 ctttaccggg agggagggct ggcactgctt taccggggaa ggatactgtt aattgaataa
     1861 tatcgtttaa attcgccccg ccggaaaaac tgtctccggc cggctgaaat gagagatttt
     1921 atattcagat atggtactgt cacactttcc atggtgttct gggccttttc cttcatctgg
     1981 ttcaaggttg ccaatgaaac ttaccggccg ctgacaatcg tttttatccg cctggtaata
     2041 tccgtaatac tcctcaacat tttcatatcc ttaagccgga gccgtatcag gataaaaagg
     2101 gaagacagga agctcttcat cctccttgca gtgtttgagc cgttttttta ttttctgggt
     2161 gaaagtttcg gcctcactta tgtaaatgca acaaccggct ctgttataat ttcaactatt
     2221 cctgtctttg cagcgaccgg agcctgggta ttgttcgggg aaaagcttaa gcctgtcaat
     2281 tatacaggga tcatagtgtc tttcatcgga atactggttt ttctccttaa ccgcgacggt
     2341 tcactctcat tcaatatcag ggggattctc ctgatgacct tcgcagtatt ttcggcagtg
     2401 ggatataacc tgacactcag ccggcttgtc ggttcctatg atccaataag cattgtcaac
     2461 atacagaata tgataggcgc cctgctcttt ctgccgctgt tcctcatatc cgatctggat
     2521 catttcatca gcacaccctt tcaggcaaaa gccctgatgc ctgttgccaa gctggcggtg
     2581 tttgcctcct gcggtgcctt tatactgttc gcctacgcgg taagacaaat gggagtaaca
     2641 aaggctaacg tgtttaccaa ttgcatcccg ctgttcacgg cagtattctc attcttcata
     2701 ctgggcgaca tactgaccct tcagaacatt tcaggaatgg caatagttat aaccggactt
     2761 ttcatgtcgc agtataacgg ccgttcgggc aaaagcgggg aggtaaaggt gctgacaggc
     2821 aaaacagcct gagcgatgct ctgtttcact tatcatatta cgttttcccg tattcagatt
     2881 ctttctatct ttgaccattg cataaaaaca tgggaaaaag aatcttgtat attttcctta
     2941 caggcattct ggccataaat tcaggtgcac aaacaggcgg cgacaatgtt tatgaattcc
     3001 tgaaccttac ccattccggc cttgtatcat ccctcggggg aacaaatgtt tcgatttatg
     3061 cagacaatct gaatctggtt taccataatc cctcccttct cagctctgaa atggacaagt
     3121 cgcttgcaat gagctttgtc agttattttg ccggaataaa ttacggacag gttatgtacg
     3181 cgctttccgt ccctcgggca ggagactttg ccgcaggaat aagctatctg aattacggat
     3241 catttacgga agcagaccct tccggaaaca ttaccggaaa attctatgca tcggaatatg
     3301 cattttccct ggcctggtcg cgggaaatcc atgagggctt caatgccgga ataaatctga
     3361 agcccgtcct ttcgcatctt gaaaggtaca gttccttcgg actggcaatg gatatcgggg
     3421 gcagctacca cagtgagtcg ggtcatttct cggcaggtct tgccatcagg aatctgggag
     3481 tccagattac cacgtatgca ggggaaaaac gcctcagtcc cccctttgag attcaggcag
     3541 gagttagccg gaagctggcc cactctcctt tcagattctc cctgaccctc agacatcttc
     3601 agaaattcaa actcacacat ctctatgatg atcctgagga aagctcacag gattccggtt
     3661 tttttgaaaa cctgatgaga catgcaattt tcggggtgga aatgattccc cacaggaatt
     3721 tttacctggc tgcgggctac aattatcaga gaagacggga actgaaaacc ggatcaggaa
     3781 gttcggcagc aggattttca tggggcttcg gaattaaaac ttccgccatg aacattgaat
     3841 tcggaagggc aacataccat cttgccggat cttcaaatca tgtttcactg atcctcaaaa
     3901 cagacaggat ttataaaaaa ctatggacaa aagaatcaca atagccatcg acggttactc
     3961 ttccagcggc aaaagcacat ttgccaggga aatagcgaaa gaactgggtt atatatatat
     4021 tgacagcgga gccatgtaca gggccgcaac cctgtactgc atcagaaagg gccttgccgg
     4081 cagggatgtg atcgatattc agggtatcgc ggaagcgatg aaggagatcc ggattgaatt
     4141 cgtctataac cccgattcag gtgagagtga aacttatctc aattcggaga atgtggaaaa
     4201 ggagatcagg ggacttgatg tttcggccca tgtaagtgca attagcaaaa taagcgaggt
     4261 ccgcacacgg ctggtggagc ttcagaaaca gatgggtgca aacaggggca ttgttatgga
     4321 tggaagggat ataggcacca cggtgtttcc ggatgccgat ctgaagatat tcatgactgc
     4381 cccggtcgac atcagggcaa aaagaagata tgaagaactt aagaacaagg gtatagatgc
     4441 cagcttcgaa gaaataagaa acaatatcgt tgccagggat atcaccgacg agaacaggga
     4501 tatcagtccg ctgcgcaggg ccggcgacgc ggtcattctc gacaacagca gaatgagtat
     4561 cgccggacag atggaatggg ttcggaaact aattgaagaa aagctgcatg atcgttgaaa
     4621 ctgacaattt ttcgggattc tgtttcggag tccgcaacgc tgttgaaata gcagaaaagg
     4681 ctctcagggc cggtgaggag atttattctc tcggactgat tgttcataat gaagcggaaa
     4741 tcaacaggct tgttgccctc ggactaaaaa tgatcagcca caaagaactg gacagcctga
     4801 aaaacagcaa actgcttata agggcccatg gtgaacctcc tgaaacatac cgcacagcag
     4861 aaaaaaacaa tattacggta attgatgcca cctgtcccat tgtaaaaaaa cttcaggcaa
     4921 ggataaggga aacatggctg aagtcacggg agaacggggg gcagattgtg atatacggca
     4981 agcccggcca tgccgaggta atcggattgc tcggacatat aaacaatgac ggcattattg
     5041 tttccggccc cggggatctc tccggaatag atttcagcag accggtcatg ctcttctccc
     5101 agaccaccat gagtgttaag gaatacaaca ggatagcaga gcttatacgg gagagtatgg
     5161 cacaaagcgg aatcactgat acggacagtc acctgaaggt tcataatacc atatgtgccc
     5221 aggtatcggg aagggaatca cagctgagat cctttgcaca gaagcatgac attataattt
     5281 ttgtaagcgg gaaagacagt tccaacggca aacttctcta ttccgtctgc aaatctgcca
     5341 accccgacag ctggtttgtg tcttcccccg gggaaataga acgatcatgg tttgaaggga
     5401 aaaaatccgc aggaatttgc ggggccacct ccacaccaaa atggcttatc gaggaaatac
     5461 gcaatcttat aatcagtttc tgacagctcc gttgtaccat attctgatat taatattttc
     5521 tgactatttt tgcggtatta aaataattcc atggaaaaat caaaggttta ttttactgac
     5581 ctgagaataa aacccggcct gaatctgctg aaaaaactta acaatctgat tattaaagcc
     5641 ggaataaaac aaatagagtt tgaaaagaaa atagtggcag tcaagataca tttcggcgaa
     5701 cccggtaatc ttgcctatct gaggcccaac tttgcagcac acattgtgaa attcctcaaa
     5761 gcagaaaaag cacttccctt cctgaccgac tgcaacacat tgtatcatgg aaaacgtgca
     5821 aatgcgcctg aacatcttga ggcggcattt gaacacggat acaatccgct ggtaacggga
     5881 tgcccggtaa taattgccga tggaataaga gggggtgaat accgcgaggt ggaactgaac
     5941 ctggaactta cccgctcagc taaaataggt tcggcaatag cagactccga catcattatc
     6001 tcaatgaatc acttcaaggg acatgagctt accggattcg ggggagccct caagaacctg
     6061 ggaatgggct gtgcatcggt gggtggcaag ctctatcttc attccggcac ttcaccggta
     6121 ataacggagg agaactgtac cggatgcaaa atgtgtgaaa gatactgtgc ccatgatgca
     6181 attaaagtgg agagtgacaa gatagcacgt atcgactatg aaaaatgcgt gggctgcgga
     6241 cagtgtgttg cagtctgcca gtacaacgcc gcaggcttcg tacttaacca ttcctccgaa
     6301 cttgcctgcc ggagaatatc cgaatatgcc ctggctgttg taagggacaa accctcgttc
     6361 catataaact tcattctgga tgtttctccc gattgcgact gctgggatgc aaatgatgtt
     6421 cccatagtcc ccgacatagg catggcagct tcatttgacc cggtggcact ggacaaggcc
     6481 tctgccgaca tggttacctc ctcacccggc ctccggggaa gtaaaatact cggaaagcgc
     6541 agtgacggcg atctaagcgg aatggacaag ttcaggatga cccatcccga cacccactgg
     6601 cagtcaggta ttgaacatgc cgtgaagata ggcctgggga atgcggatta tgaactgata
     6661 cacgtataat attattaaaa gccaggataa tacctcagaa aaaccaaagc tgattccggc
     6721 cagaatgaat ctgaaagtta aaacagcacg gctctccatt atttcgaata ccctgcttat
     6781 agttcttaag ctgggggcag gcctggcaag cggttccgta agcatcatct cagaagcgat
     6841 tcactctttc atggatctgc tggcagctgt tattgcattt ttctcagtga agatctcgga
     6901 taatcctccc gatgccagac atccttacgg acacgggaaa gtggagaatg tttccggggt
     6961 tattgaagct ctcctgatac tggctgctgc cgggttcatc atcattgaag ccgtgaagaa
     7021 actcacggga aagccattcg atctggaatt catctgggca ggtatcatcg taatgatcat
     7081 atcctcaatt gtcaacttca ttgtatcccg gagactatac agggtggctt ccgaaacggg
     7141 ttccattgca cttgaggcgg atgccctgca tctgaaaacc gatgtttaca catccctggg
     7201 tgttgcagcc ggactggggc tgataatgct gacaggaatc aaatggctgg atccactggt
     7261 ggcaataatg gttgccggac tcattatcta tgaggcgttc aacctgctca ggaaggcatt
     7321 ctggccgctt attgacactg cctggaccga tgaggaaatc aggctcctca agaaaaaact
     7381 cgaaatgatg aaggttaatt atcacggtct cagaacccgt attgccggaa actacaggtt
     7441 catcgacatc aatgtcgtga ttccggcaga acactcggtt gctgatgcac acaggttctg
     7501 cgacatgatc gaggatgagt tgtcgaggaa ttacgagaac ctgacggtga acatacacgt
     7561 ggaaccgtca tgacttatct cgaacggatc tcccttctca taaaagttac ataggataat
     7621 acaaagcaga aaacggtagc tgctataagt ccagtcagct gaggccatac cagcagaagg
     7681 ctctgtccga gaggcagcgg acccggaact gctcccacaa tctgttccat ggtaaggggc
     7741 cccaggctcc ttaccgaagg catcagaata gttgttgttg catcactgta aagctccccc
     7801 ggcaatatct tcatcagtgt cataattatt tcctggtagc ggaatacctg atgtgtgctt
     7861 gccattgccg aaggtgctat agccttggct atcagattga cgataagatt gtaaaaaacc
     7921 gtgagaaaga gccacacggc gatacatgcc agggcggatg tggcaggctg acgaaagatt
     7981 accgagaaca gaatcgccag attaagccag aaggcaacat ataaaatgct tactaatatg
     8041 aagaatacaa ctctccagaa ttcctccggc gtcgggggaa gccccgtaat gataattccc
     8101 agtcccataa acagaaatcc gagagcaaag aacagtacgc tgatcactgt gagcgccgac
     8161 acgaatttcg cgttaatgag ataatcacgg tgaataggct gtgacatcag ccggcttagc
     8221 gttccctttg tctgctccga gttgacggtg tcaaatccca gactgattcc gagcaacggc
     8281 cccagaaagc ttataaacac gaaaaacgac ggaagagtac cgtcagaaat ggtgtaaagc
     8341 ttcaggaaca gaaaatttcc ttcctgatca ttggcctgta ccgcacttcc tatgtttgtc
     8401 agggccgtgt acatcgaccc gaagcaggta agaccgataa tgaaaagcag tattataaag
     8461 cgccagctgt tcacctggtc ggaaatctcc ttgtggacaa tcaccctgaa aggatgaaca
     8521 acctcgtcag ccttccacag ttttttcctt ttcttcactg ttgctgtcat attattcatt
     8581 attccccctt ccttcaaaat aacggtgata aatatcatcc agcccgtact cctttttgtt
     8641 caggtaattg agaccggcgc cggcttccac aacccttgcc gctatctcct cagccacatc
     8701 acgcctgcat tctacaatgt attttccata attcagactc accttcccca caccttcaat
     8761 atttccgaga aggtcccgaa gccaccttgc actgttctga tcatgatagc cctgcggagg
     8821 gtttctcagt ccgacttcca ccgtgtacgg actgccggaa aaaagattct ccgacagggt
     8881 ctgtatgtct cctgaagcca gaagcctgcc ttccacaaat atcccgacac ggtcacaaac
     8941 ctgctgtacc tggtgaagat gatgcgaaca aaacagtact gttattccct gttccctgct
     9001 gagacgcaca atcagatcca ggaactccct tacaccggcc gggtcaatac caagagtggg
     9061 ttcgtcaagt atgattattt ccggattctt gataagcaca tcggcaagcc ccagcctctg
     9121 cagcattccc cgtgaatact tcagagtctt cttgccggaa tgttccgtca gaccgacctt
     9181 ttccagaagc tcctccgcct tttccattgc ctcgtttccc gtaacagcgt tcagacggcc
     9241 tgtaaaaaca aggttctgga gtcctgtcat atcctcatag aaccctacat tctcaggaag
     9301 atatccaacc tttttcttga cttcagtcgg gctgcttgtt gaatcaatcc cgcataccct
     9361 cacgcttccc gaagtgggat cagtcagccc gagcatcatc aggatggtgg ttgtctttcc
     9421 tgcaccgttg ggcccgagaa gaccaaatat ctcaccttca cgtattgtca ggttcagctg
     9481 gtcaaccgca gtgaaagatc cgtatttctt cgtcaggtcc ctgagttcaa taatattttc
     9541 cgccaccctt tacctccttc cgtatttacg gaacagaaaa taaacgctgc ccagtgccac
     9601 ggcaataaca gccacaccta tccatcccag caacatgggt gtctttaccg atattctgat
     9661 tgtagccctg gaagacgctt cgggtgtctt cgcctcaaga tttgccacat aatcccctgc
     9721 aatcgccttt ttgtctgctt tgatcgtggc aaaaaccttg ctgttgctgc cggcttctat
     9781 cctgtcaatc ttcggagggt caaaaaccac atcccagtta acaggtgcag aaaaggacat
     9841 ctccacatca tcaagaccag atgaaccggt atttctgacc agaagctcaa tccttcggga
     9901 atcgcccgcc gttattccgg tgctgagcaa tccgctgggt gttgtgagtt caatattgaa
     9961 cgatccggtt attacaactt ccagatccag atttgcagaa gtgacatttg tagctgcaac
    10021 aacaggtatc tgatatgttc ccgcctggat attcacagga ggtttaatct caattgttac
    10081 attggaagta ctgttcggtt caatctccac cgatgtggcc tgattgtaat tcggtttgaa
    10141 tatgacattc caccctctcg gggcattggt tctgaaggca taggactgag tctcccctgt
    10201 tctgttcctg agctccgtag aaaaagtgaa ggttgaagtg gaatgaccct gcatattcga
    10261 ctgttttgtt gtgaattcag tttcatatgt accctgctcg gtcaccacaa taaccagagg
    10321 cagactcgca ccgccgcctg caacgactga aaatccgtag ctgcccttgt taaccttcat
    10381 gggaacatcc accttcaggg tgagtgtctg tttctgctca ggcagcaccg ctatcctgcc
    10441 aaccttgtat gagcctgatg tgagagaata gacccaggtg cgcggaattc ccggtaccga
    10501 aatatccatg ttccggactc ccttgctttt gtttatgaca tcaatactgt atgtgatcga
    10561 ttcacccgga ggaacggata tcctggtatt cggagtgaat aaggttacac tgtcagctga
    10621 tgcaccggct gtaatgccgg ataaaaaact tccgaaaata ataagcgctg caaggctaaa
    10681 agatttagaa tactttaaca taagaaatcc tccaagtttt aggcagttaa tacggcagac
    10741 aaatttaaca tttgaaaaga aatatctgta taaatcatat tcttaaaaat ttgttaaaat
    10801 tgactgaccc tgcctgttac ctgctgcaat attcaatgca tccggcattg ataccctgcg
    10861 gatttgtatc tttgcacaaa atcttttcag aaatgttcaa accgtataag aatattacct
    10921 gtttaaataa ttgcggatga gctgagaggt acagcgatgt taatccggtc gggaggatat
    10981 ggggacagca gttaagggga acaaaagaag gaaataaaaa acagaaaccg gagagaaggg
    11041 aatgaaaaga gggaaaaaac gcggagtaaa ggcggcaaaa gcaacagaag ctgacgaaaa
    11101 acaaaaaagg caaagaaagg caatgaaagg caacgaagat gacaaaataa agggcaacga
    11161 ggaaaacaag gacaaaagta cggtatcggg attgcgggaa ttaatgaaac tatacaggtt
    11221 tctgaagccc taccgctgga ttttcatcct tggattgttt ttcctgctca tttcaagtgc
    11281 ggcgagcctt atgttcccga aatatctggg gaacatggtg gatattgcaa acaggggaaa
    11341 tctggcaggc gaaataagca gaacaggact catactgctt gcaatagtga tcacccaggc
    11401 aatattttcc tattcccgga caaggatatt cgtttacgtc acggaaaaaa cactggcatc
    11461 catccggcag catatttaca atcacctgat aaaaatgccg atggcctttt ttgccgaacg
    11521 cagggttggt gagcttaaca gccgtatttc ctccgatgtg gctatcctac agcattcact
    11581 cacagaaacc cttgcggatt tcatgtcaca gctgctgata atcaccggcg gcatatccct
    11641 catgatggtg acctccttta aattaaccct cttcatgctt gccatcgtgc ctcccatggc
    11701 cctttttgca tttttctcgg gaagggcaat acgcagatat tcaaaaaaag cccagtcgca
    11761 tgttgcagaa tcgaatacca tagttgagga gaccttgcag ggcatacaga atgtgaaggc
    11821 attcaccaat gaactctttg aaataaacag ataccgcgac aaaaccgatg aagtggcccg
    11881 cacagggata aaagggggaa aataccaggc agctgtctcg ttcataatac tgggattctt
    11941 cattgccatg ggacttgtca tctggagggg agcccttctg atagcaaaag gtgaaatgga
    12001 agccggacag cttttctcct ttgtaatcta ttcagggttc attgcaggaa acatagccgg
    12061 aatggctggt gtttacacaa gactgcagag gacgatcggt gcggccgaaa acctcctgct
    12121 cctgatggac gaagcaacgg aaagaattga cgagcagtat atccccgatc ctgactatat
    12181 gattcacgga caaatcgatt tcgatcatgt ccatttccgc tatccttcaa gggatgagat
    12241 gacagtcctg gaggatgtga gcttcagcat aaaacccgga caggaaattg ccatcgtggg
    12301 accaagcggt gcaggaaaat ccacgatagc atccctcctt ttaaaattct atgagcccga
    12361 caggggaagg atacttttcg acggccgcga cagcagggaa ttccccgtaa cagccctccg
    12421 ctcacagatg gccgtggtga tgcaggatgt ttttcttttc gggggcacaa tacgggagaa
    12481 tattgcatac ggcaaacccg gggcatccga tgaggaaata attcaagcgg cggtaaaggc
    12541 aaatgcatgg gacttcatca aatcattccc cggaaagctc gacactgttg tgggagaacg
    12601 cggggtacag ctttccggag gacagagaca acgtatctca attgcccgcg cagtgctcaa
    12661 agatcccata atccttcttc ttgatgaagc tacttcttca ctcgattcag aatccgaaag
    12721 actggtacag gaagcactcg acctgctgct gaggggaaga acttccctcg ttatcgcaca
    12781 ccgcctgtct accatcatgc atgccgacca tatctgcgtt ctggaaaacg gaagaataat
    12841 cgaggaggga gatcacagca gcctgcttga aaaacaggga atgtaccatg ccatgtggag
    12901 acagcagacc ggcgaaaggg aaaacagcaa aatgatcagc cggactgaag taatttaagc
    12961 ccggaactga ctaatccggt tttttaatgt acctttgata tctgcctggt ttttcaatct
    13021 tgtgcattaa tctgatcatt ttccaggatt gattcatcac ccattccatc atttataaag
    13081 ccggcaattt atttttatta ttaaccttca ttgaaatttc atggcaagat cgcaggagac
    13141 attcgggaaa aaggaagtaa gaaacaggaa agagaaaaag agaaaggaaa aggaacagaa
    13201 acgcgcccaa aaaagaagtg aagggaaaaa agccagcttt aatgacatga tcgcatatgt
    13261 tgatgaattc ggaatcattt catcaacacc ccctgacccc ggcaaaaaaa cagaagtgga
    13321 tgctcagagc atagaactca caataaggca taacagtccg gaaacagatc ccgctcccgg
    13381 gaaaaaaggc gttgtctcct ttttcaacaa atcgaaagga ttcggcttta tccgcgacag
    13441 cgattccggt cagcggattt ttgtacacat gaacaatctt cttgaaccgg tcgccgaaaa
    13501 cgatgaggta agctatgaaa taaccagggg caccagggga ccgtctgcaa ttaatgtcag
    13561 gctgctcagg gaataggccg tagtgagcaa caggacaggt cagtagaaaa agattccccg
    13621 gaatattaat gtaataagcg gaaaataatt gaacaataga gaggtaaaac tgcagacatg
    13681 aaatctgaag ataacagccg gaatatggac ccggagccaa tgttctcggt aataacggcc
    13741 agctacaaca ggggcggact cattgaaaga gccctgaaat cccttacagg acagagcgaa
    13801 aaggactggg aagggataat agtcgatgac ggaagcacag atgacacata ttccaggata
    13861 tttcccttcc tgaaagcgga tagcagaatc aggtatttca ggaaagaaca cagtggcgaa
    13921 gccctgacaa agaattacgg catcaggatg tcgtcaggca gatatatcac cttcctcgat
    13981 tcagatgatg aataccatcg tgatcacctg gcatcaagga aaaaaatcct gttacagaat
    14041 cccggtatcc gcttcctgca cggaggcgtg gagatactcg gcaaccagta tgtgccggac
    14101 aggaatgatc cttccagacc gatacatctg aaagattgca tcataggagg cacattcgtg
    14161 atcgaaaggg aaacaatgtt atctcttgaa ggattcagaa acatcaaact gggccccgac
    14221 agtgaccttt ttgagagagc tctcaggaga ggaatcccca tgatgaagct tgatctgcca
    14281 acctatatct atcatcatga aaatcccgat tcaataacaa acatcctctt cgggaaaata
    14341 caggatcact gaaaagcagg cagttcacgc tgcaaacctg acgaaaagaa agtcttcttc
    14401 accattataa accaggtaat tgaaaaaaca atcactccca ggtgcagcct ggcaagaatg
    14461 atatcagata tcattctcgg acacaaaagg tcaacaggca ggactccgag cagcaataaa
    14521 tttatccaaa gcagtatttt ttcaggacgt gcaggaaata tgcttgaata ccatattgcg
    14581 tatcccgccg cggcaaccac ataggtatgt ctctccgaac ctgcactgaa cagaatgacc
    14641 cagctcatca ggagtcccag gtactgagcc cggagaataa aagattccct gaatattttc
    14701 agccgtatca gcgtgaatat ggcgataact gcaaacactc ccagcgatat gaacccggta
    14761 atggaggcaa ttgaaccgtc aacagccagg aaaggacggt aaatgctgta tttgttgaat
    14821 ccttccagat ggtttgtcac cagaaatatc caggaattat aataatcaat aagttccggc
    14881 aaagggatcc ggaccaatgg caggagaaac aggaaagttc cgatgagaac agcgtaaaaa
    14941 atatttctcc acagccgcgg gtaaaacaga agcattataa tctgaaaaat tccgtaaact
    15001 tttgtgaatc ccgataccaa tatcagaagt accgcccaga atcctctgtt cttttccaac
    15061 agggtgaaag agaatagaaa aagagccgca actaccggat taaactgcac cgacagcaat
    15121 gttgtaaaaa gcagaagcga ggaaaagaaa aagatgaatc ttttggtatt gaaggaaaat
    15181 ttttcgggta aggtgaaaac agaaaaataa aacagggtat atgtgaatat gttccacaaa
    15241 tatacacctg cccagccagg aataagggtg aagggcgtaa acagaattga gaacagaggt
    15301 ccgtaaataa aaacatcaag ctgtttgccc agaatggaaa gatgattcca gtccgaatat
    15361 ggatttatgc cattccagaa gtcaagagaa ccataagaaa agatctgaaa gttgttgtat
    15421 atctgacgga tcacttcatt tgaagtaaca agaagcgcta ttatcaggcc tgtgaaatag
    15481 attctttttc cggtagcggt cagcctgtcg agcagtgaca ggaatttatc cgtattcatt
    15541 ttactgatct tgtttatttt taatattttt caaaactaat atttttccgc caggagaaaa
    15601 tctgtcgggc ctggttttaa gggttaccgg atccacgggt gaaacaggac aattatcccg
    15661 tactgagtga cacggaaatg tgttttccct ccgattacgg ctttatatta acaggtttaa
    15721 atcatttttc tgatttgcag gaattcggta attccaataa attctccctg cctcttcaga
    15781 cttaaaagaa gcctttccat cagatttgtt aactgatccc cgtcttttct tctgatatag
    15841 aaaggaattc taaagtgacg gatatccgca aattcccatg aatggaagta aaggtgcagg
    15901 tatttatcct tgtcgagtgt ccgcttgcac aggaaagcaa atatgccgaa ggggagattt
    15961 ttaaaactaa gccagaagag cgggaatctt attaccggac taacagacac ggggacaacc
    16021 ggcagaccgg tagctttatc cctgaacaga gtacggggac ttgaaagcat gttgtacctg
    16081 aagggaacca gggtaggatt gacagatgca tcataactgt aaccatattg cttcagttca
    16141 ttcaaatcgg tttttgccat cctgggcata cgaaatccgt ttattttctg cctgcttatc
    16201 tcctccagta tattttttga ttttccagga tcgcccggat gaaatatcgt atgactgtgt
    16261 gaatgcgaag atatttcatg tttctctgct atcttcctga ccattccggc attggcttct
    16321 gcatagcctg aggtgataaa aaaagtcgca cgtatattat gtttttcaag caaatcatat
    16381 attcttgaaa ggcccttgtt tgtaacggac atcatcctgc cggatggcag acttatgccg
    16441 tattccaacg gcagatcaaa ttcctccaga tcaattgtaa acaatatata tttagacccg
    16501 tccaacaatt attttcagga aattgaccag ttcccgcctt aatactgcag aactgaagtt
    16561 gttaaatctt atttcagggt ttaaatcaac cttgacaaaa ccaaatttaa taccactctt
    16621 taagcatttc agagcaaact ccagatcaaa gacaaagctg tttgttttcg ttgtcagaaa
    16681 aatactcctt gccctgttgt caagcccttt taatcctgcc tgggtgtctt ttatcctgaa
    16741 ccccgtgata caatagttta taaggatcag cgacaatgat accaatcttc tgataaacgg
    16801 aagccttctg atgaaggtat tgaaattcct ggatgccatt acaagatttg tactgcctcc
    16861 tgccagtatc tcatatattt ttctcagtac atcagtgccg aatggaaaat caaagtcgca
    16921 ataaatataa taatctgatg ctgaacgttc cattgcatgg cggattgcaa atcccttgcc
    16981 catattccgc ggatagcaca gatatttaat atcagggtat tctggcagga ttccgtttct
    17041 gatgatatca tcaacaccat cagtggaccc atcattgaca attgttatct gataatcaac
    17101 gccggaaaat atttcctgaa ttttccccag cgcacataac aattctccct cccacccctg
    17161 gtgcggatta tatacaggaa gaatgattgc cagatcccgc ttcgtgacca tgaattgata
    17221 gggattttct ttcagtacat tacaatgatc actgttaaag tccttactgc caggtttaaa
    17281 atactgcttc aggttataag gcccaatata aaaataataa ataaaaatac tatcacagga
    17341 gaggaaattc ctcaaatttt gttgaaatca ggagaaccgg gcgtgctgat tccagctgtc
    17401 ttgcccagtg gagtgtctgt ccggtacttg tgtcagacgg gtaaaatccg gattaaaaag
    17461 tccttattgt aaataaaaga taaatcatat aacttgcgca acgtttttat caccccggca
    17521 tgaacaagat ttatttttta gcactattaa ttctgatgac tggcggtctg gctctgaacg
    17581 gtcaggcagt tcagcagttt taccataaca ggattgaaaa cgccattgat gagatcagca
    17641 gtactgaagt aaattccggt gcggtggtct ggaagctcta taatcatact tacatcatta
    17701 aaacaccgtc tgttacaata ggctttgata tccagagagg tattccggga aatgaaaact
    17761 ttgccttcag caggaaaata acggaccggc tgataatacc gggacatgaa aatgaactga
    17821 accacagcac agatcaccgt gaaccctgct ggctcaacta cacaaggctg gggaacgatc
    17881 ctgcccttcc ctggatacag atggctccgg gagaaaaata tcattataat tcccggatgc
    17941 aataaactgc ccgggactgt ttttactgct ttcatgcgaa ccttatagaa cttatattgt
    18001 taaacggcaa ataaatttaa aatcagaaaa aaatggaaga cattgaaatc tgccactgta
    18061 acggcataat gaaaagtgaa attgttaagg caataaaaga aaagggatta aagaccgtcg
    18121 aggaagtaca ggatgaaaca gaagcgggta ccgtatgcgg cggatgtatt cccgacattg
    18181 aagatatcct cagtgaaatt aacggctaaa aggagttatg gggaaaaacg aatttgaaaa
    18241 aggaaaaaag ttctctttcg cggacagtat tacatatgct gaaaaatccg tggtaagcaa
    18301 acacgtactt aaaaaagaga caggaaatat cagtctcatg tcctttgaca agggggaagg
    18361 cctgactgaa cacacttcac cctttgatgc agtggtgcat attgttgatg gcaaggctga
    18421 catagttatc gacggtgtct caaatatcct tgaaaccggg gataccgtca tcatgcctgc
    18481 caatatcccc cactccctgt ctgctgtcga aaggttcaag atggtcctta tcatgatcag
    18541 aagcaaacaa tagacagctt ttatcaagca tggtaatagg tatagacatc ggcagcacca
    18601 caaccaaggt ggcggcaatc atgcccgaca aggagattat ccggataaaa acaagggcca
    18661 ttgatgccat tacctcggca acaggagcat ttgggaaact gattgttgaa aatcatttca
    18721 gcctcgacag cattaaaaag atcaatatca ccggggtggg tgccgcaagg atcaccgaca
    18781 atctgttcgg cataaaaacc aacagggtcg gggaaatgac ggcaatagga accggcggca
    18841 aatacctggc aaaaaaggac aggatcataa tttcaaatat aggaaccgga acagccatta
    18901 tagaggttga cggcagcaaa atcaatcatc ttggcggaac cggagtcggc ggcggtacga
    18961 ttatcggcct ttcaaaaata atgctcggaa caaccgattt cgatacaatc atgcaatatg
    19021 cctcaaaagg atcaatcaac aatgttgacc tgctgatagg tgatattgcg gataccaata
    19081 taagcttcct cgataaggaa ttcaccgctt ccaacttcgg aaaaatgctg gatattgccg
    19141 gaaaggaaga tctggctatg ggaatattca atcttacata ccaggttatt gccatggttt
    19201 cagtgtttgc agccaaaagc aagaataatg ataccgttgt ggtt
//
LOCUS       DAOSPX010000024        18008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3254_length_18008_cov_4.155350, whole genome shotgun sequence.
ACCESSION   DAOSPX010000024 DAOSPX010000000
VERSION     DAOSPX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18008
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_18008_cov_4.155350"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /gene="murD"
                     /locus_tag="PLZ75_02365"
     CDS             <1..1032
                     /gene="murD"
                     /locus_tag="PLZ75_02365"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005649514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOS71273.1"
                     /translation="IKICVTGSNGKTTVTNLIRHMLEKAGKKVAMTGNVGNSFAMSVA
                     REHYDYYVIELSSFQLDDMYDFRADIAILLNITPDHLDRYGYDFRNYIDSKFRITRNQ
                     TKSDYFIYWADDPVITKELERRKYDMTLLPFSDRHTEKMAAYIDHEELIIDYQNKTTK
                     LMTIHELALKGRHNIYNSMAAAIAGRVLDIRKPVIRESLTDFQAVEHRLEPVIKVSGI
                     NFINDSKATNVNSTWYALECMETPVVWIAGGIDKGNDYSELFEVVKQKVKAVVCLGKD
                     NAKIIDAFKDKVKTIIEAASMEEAVRSSYYLAGKGDTVLLSPACASFDLFVNYEDRGR
                     QFKAAVRNL"
     gene            1044..2255
                     /locus_tag="PLZ75_02370"
     CDS             1044..2255
                     /locus_tag="PLZ75_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOS71274.1"
                     /translation="MFQGWLSRTFKGDRAIWGVVAVFMVYSLLAVYSSSVNVAFRNHG
                     GNTTYFLRSQFLMLTAGLLIIILVHYLPYRIYSALAGIAVIVSAILLVLTYFFGVTIN
                     EATRWLEIPYIGFRIQTSDIAKVALVIYLARSLARYQNQLKDFVLVTKFLMVPVLIIC
                     ALIMPENLSTALMVFGICILIMFIGGVPVKFLIAYAGMAVVFVIFFALVLWLLKEDNR
                     VLTWIERMISFFAGKSSGADDYQINQAKIAISTGGLFGKAPGHSIQRNMLPQSNSDFI
                     FAIIIEEYGLLLGAIPLIMAYMILLFRGISIVKKCETAFPAYLVLGLIVMIVTQAMLN
                     MMVAVDIFPVTGQTLPLVSWGRTSVVMMCFSIGVILSVSRVVNARLRKEELPEEEQKD
                     EGELIYGTVAK"
     gene            2245..3348
                     /gene="murG"
                     /locus_tag="PLZ75_02375"
     CDS             2245..3348
                     /gene="murG"
                     /locus_tag="PLZ75_02375"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009237085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOS71275.1"
                     /translation="MRNKRIIISGGGTGGHIFPAISVANALRRIEPGTDILFVGAEGR
                     MEMEKVPAAGYRIRALPVSGFYRQITFRNFSVLLRLIRSLSMAKRIIREFRPDVALGV
                     GGFASGPVMWKAAGMGIPVVIQEQNSYAGVTNRLLAKRASAICVAYDGMEKYFPRDKI
                     IKTGNPVRHSFDNSGVLKNEALEYFGFSREKQVVLVLGGSLGAGTINKTLGGALDRMA
                     GSGLQWLWQTGMNYYEDVKSLAADSLAETIAIHGFIDRMDYAFAAADIIVSRAGAGTI
                     SELCLVGKPVILVPSPNVAEDHQTRNAEALSGNGAAILIADSQAEKKLIDEVIRLSLD
                     TSGKAGLSENIRKMAERDSDIRIANEVLKLAGT"
     gene            3345..4742
                     /gene="murC"
                     /locus_tag="PLZ75_02380"
     CDS             3345..4742
                     /gene="murC"
                     /locus_tag="PLZ75_02380"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOS71276.1"
                     /translation="MNDLNNMEGVYFVGIGGIGMSALALWFRHRGYVVAGFDRTQSSI
                     TDSLAAKGCEIVFEDDPSALPELFREKIPGKEILVVYTPAIPAESSLLSHFREKGFRL
                     RKRAEVLGTISRSTDTIAVAGTHGKTTTSTMTAHLLKQSRVDCSAFLGGISRNYDSNL
                     LLGNSRFTVIEADEYDRSFHQLDPLMAVITSVEADHLDIYGDLKTMTEAYDVFCGKIR
                     PGGLLLLNHRIRDRLKHPEDVSVFTYGNDPGADYRYFNVVSGEDYYLFSLSTPEGMIE
                     NLRFHFPGRINIENLTAAIAIALKCGVSETEIRRSVPLFKGVRRRFDIRVNMPGLTYI
                     DDYAHHPGEIKACIRSLREYFGGRRITGIFQPHLFSRTRDHADEFAEILDELDEAILL
                     PIYPAREKPIEGVSSELIFERMKLENKKMLQPQNVVDMLDPGRLDVLVTIGAGDIDRL
                     VDPIERKLRGENPEK"
     gene            4788..5570
                     /locus_tag="PLZ75_02385"
     CDS             4788..5570
                     /locus_tag="PLZ75_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71277.1"
                     /translation="MMRLMKILLIIPLIYMLAAPVWLSSYYGSRPCRDISIVICDSSD
                     YDFVTRKQLLSLVNGREGSLLGRPQGSLPLWDIEGRVRTLKELRKAEAYMSIDGILNV
                     DVYQRNPVMRVIPDEGGDFLVDAEGVLIRKRTLQSPRLHIVGGDIAISRAMLEGTSIL
                     DAGHTILKDAYHFVRYISGDDFWSAQIDQIYVDKRGKIDLIPRAGNHRIHLGTFENFE
                     GKLKNLAAFYENVIPETGWDKYSVISLEFRDQIVCKKRQQAK"
     gene            5581..6933
                     /gene="ftsA"
                     /locus_tag="PLZ75_02390"
     CDS             5581..6933
                     /gene="ftsA"
                     /locus_tag="PLZ75_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOS71278.1"
                     /translation="MSKREQYIAAIDIGTTKIVSIVGKKNENGKIEILGLSKALSRGV
                     KRGVVINIEETVTAIQTTVEDVQKRSGVKFSEVFVGIAGQHIKSMKSRGYITRDSYED
                     DIKKEEVFRLIEDMHKIHVEIGEEIIHVIPQNFIVDDETGLKSPIGICGRRLEANFHI
                     VIGQVAAAKNIEKCVRRANLSVKDLILEPLASSDAVLTDDEKEAGVVLVDIGGGTTDM
                     AVYYDNIIRHTAVIPLGGNVVTNDIKEGCAILHRHAEQLKIQYGSALGDIAPDDKVVA
                     IPGISGRNPKEISFKSLAYIIQSRMEEIIDFVNFEIQNSGYADRLAAGIVITGGGAML
                     KHLPQLMQFRTAMDVRVGYPNEHLGGAGREEINQPMYSTSVGLIMRGFDFLETYRKSF
                     NAAPQHEFTEQGGGSFEPASVPGEDQADTAAEPKTSLTEKIKMLMQKMFEVEDNPINQ
                     "
     gene            6968..8476
                     /gene="ftsZ"
                     /locus_tag="PLZ75_02395"
     CDS             6968..8476
                     /gene="ftsZ"
                     /locus_tag="PLZ75_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOS71279.1"
                     /translation="MEKKMILSENSDDLINFDLPVERSSIIKVVGVGGGGVNAVNHMF
                     EKGIKDVNFIVCNTDRQSLDKSPVPVKIQLGASLTEGMGAGSQPEKGRKAAEENIADV
                     MNALSGNTRMVFLTTGMGGGTGTGAIPVIAKACREAGLLTIAVVTIPFKSEGKIRINY
                     AVEGIAELENHVDSLLVINNEKLRDIYGNLGVSSAFGKADDVLATAVKGIAEIITVTG
                     YINVDFADVETVMKNSGVAVMGMGIAAGENRAIEAMENALASPLLNSNDITGAKSILI
                     NISSGRAEDELTMDELGDITDYIYSVASDDALIIRGLSSDEELKENLSIIVIATGFEA
                     NNIIQPMKPRRAKKVEVLSSGDYPLNPGRETEKAPEEFRVHGKENEPIIADFRENYQG
                     TLDFSVETARGRKSSADRDNDHEGGEDPELRLKKVKHMQKIMQKEGISSRTMKENIEA
                     FEDVPAYIRRNMSLPAPDRQSGSELSQFTLSSDENDGPVISKNNAYLNDNVD"
     gene            8548..10464
                     /locus_tag="PLZ75_02400"
     CDS             8548..10464
                     /locus_tag="PLZ75_02400"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOS71280.1"
                     /translation="MIRNLKILFILLILTGSGLSAQKSAGTRTIRQWNLSHDFTEETI
                     IPFDTVFSLFHRYRIADRYSPLNATLGNYGLPFYQINFFDRITDPDEFLYSYYLPVMY
                     VPRKALFMNTQVPFTEMVWTNGGPRQMSEQTFRVRHSQNVNRYLNFGLVYDIVFNLGQ
                     YSYQKAVDKNFTFFSSYTGPVYKYYFSAGINNITSYENGGINDREDLDLFDTRDVPVR
                     MGGLNSARSGLKNKNMLLVQRYTVGRKTGTADSLNSSRDFSGLRGIFSHIFIFDNSGR
                     TYSDKYPQSGFYDTIYINNSFTYDSLSQRIIKNTLRFDFSTDEKRKFRVGGGAGIRNE
                     LIRYSQICPLPDTLSADTLVWRKPNNVLTGRLYNNIGEKFMWTADGELFLTGYRAGDF
                     RLKGELVKSFEWKKGTAVWNINGSIMNRQPSFWYERWGSNHFSWNAGMKKEFRVDIGS
                     RFSYPAAKTDLRFNYAIIDNYTDFNPESLPSQHGGGLSVLSVMAGKELTAWKMHLSGD
                     LLTQLSSNPAVLDLPLLSVRTAGYIEHLFRFRQTNGSLNSQFGIDLLYHTPYRAYSYM
                     PATGRFFRQEKTKTGNYPFVNIFLNLKLKRTRIFVMLDHVNSGLSGSDYFMIPLYPMN
                     YRMLRYGIAWTFYN"
     gene            10527..12023
                     /locus_tag="PLZ75_02405"
     CDS             10527..12023
                     /locus_tag="PLZ75_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOS71281.1"
                     /translation="MGKITIFITAILAAGLISCTDGKIHPPETDPSNLSSDLEIIRQR
                     GKLIAITDINSTNYFVYKGEPMGFHYELLNDFADYLGLDLELITENQLDKACDLLQDG
                     KADIIAIGLTVNSARKKKILFTEPILQTRQVLIQRKPRNWRSLTAAALERQLVRNQLD
                     LANKTVFVQENSAYINRLRALSNEIGDTIGIVEVPYNSEELIRFVANGEIEYTISDEN
                     VAMVNSTYYPDIDISTPVSFPQNIAWGLKKRESSDLLNELNHWLSTYKETRRYRAMYA
                     KYFRNSRSNYMVKSDYYSLSTGKISRWDDMIKRASREIEWDWRLLASLIYQESRFDPD
                     VESWAGAYGLMQVMPPTGEKFGIDVKSSPASNMRAGILYINWLKTMFENKIPEENERL
                     KFILASYNAGPGHVLDAMKLAEKNGMDPRKWEDNVAVWLLKKSDPVYYKDAVVKNGYF
                     KGTESVNYVTQILERYEHYKNIISGENSIRLTMNTDQDSRDNSLRISN"
     gene            complement(12033..13277)
                     /locus_tag="PLZ75_02410"
     CDS             complement(12033..13277)
                     /locus_tag="PLZ75_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS71282.1"
                     /translation="MNLPYFIAERLIRGRREGTSFSRPINVIAIAGIATGLAVMILAV
                     AILTGFKQQIREKIIGFGSSIQIVNLDSNLSFETVPIRQGQEFIPKIRNLPGIKHVQV
                     FATKAGIIKTGDNIQGVVLKGVGSDYDWSYFASSMVEGEVFRVSDSTRTDRVIISRKI
                     SDMLRLRTGDSFAMHFVQDPPRMRKFVISGIYETSLEEFDKIYVFCDINHIKRLNGWD
                     DDQVSGFEIFINDFGKLDQMTSAVRSAIGYQLLRDEEKFNVISIREKYPQIFDWLNFQ
                     DINVIIIILLMLTVAGFNMVSNLLILILEKTNMIGILKALGSHDKTIRGVFLYQAAYL
                     IGKGLFWGNIIGIGLSVLQLKTGLISLDPSSYYIKTVPVNLQMIHIVFLNAGTMAAIL
                     VMLLVPSKLISRISPVKAIRYD"
     gene            13562..14725
                     /locus_tag="PLZ75_02415"
     CDS             13562..14725
                     /locus_tag="PLZ75_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOS71283.1"
                     /translation="MKYLLTFLLMVASACACKSGNEGPVPGAERPEEYMKMLDGLAVG
                     IVANQTSMAGETHLVEYLLGRGVDVRMIFAPEHGYRELAADGAHIGDGTDAATGIPVR
                     SLYGVRRKPDPEDMAGLDAVIYDIQDVGARFYTYISTLHYVMEACAENNVKCIVFDRP
                     NPNGFYFDGNIADTAYRSFVAMHPVPVMHGMTIGEYAGMINGEKWLPGGQQCELAVIR
                     CRNYTHQSLYELPVRPSPNLPNMTAVYLYPSLCFFEGTSISLGRGTPFPFQVYGSPLF
                     PDEGFSFTPESVPASVRPPLLGVKCYGVDLRNAMEEKLVPRPMVNLDWLTDAWTKYPE
                     KDKFFNNYFDVLAAGPTLREQIQSGMSAAEIRATWKDGLEKFGKTRQKYLIYE"
     gene            complement(14735..15952)
                     /locus_tag="PLZ75_02420"
     CDS             complement(14735..15952)
                     /locus_tag="PLZ75_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOS71284.1"
                     /translation="MPLLSQRSKNIPASPIRKLIPYAEGAVKRGIKVYHLNIGQPDIH
                     TPEVALNAMRNLDKKVLEYSHSAGYESYRRKLAGFYRNAGIDISWDQTLITTGASEAI
                     SFAVNSCLDYGDEIIIPEPFYANYNALASIAGVRIVSVTSGIEVDFALPPVSEIEKKI
                     SEKTRAILLCNPNNPTGYVYSAGELDQIRELVKKYNLFLFSDEAYREFAYDGQKVVSA
                     MNLKGIEENVILIDSVSKRYSECGIRIGVLISRNKEVMSTVLRYGQSRLSPPGLGQIV
                     AEASIDTPPEYFTEVNREYLARRNFIVDALNRIPGVRCPMPKGAFYTMARLPVDDADK
                     FAQWLLEEFEYEKQTVMIAPGSGFYSTPGLGRNEARIAYVLNIGDLRNAAMVLEKALK
                     VYPGRTQGDGSSK"
     gene            16216..17379
                     /locus_tag="PLZ75_02425"
     CDS             16216..17379
                     /locus_tag="PLZ75_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOS71285.1"
                     /translation="MLIAIPKEIMEEEKRVAATPETTEKLIKLGFKVILESNAGAGIF
                     YSDEDYRSAGAEIVADAETLYGRADIILKVKEPRYNVNAGKHEVEMMKDESILIAFLH
                     PATPSNHSLVKMLRDKKITSLTMDGIPRTPNAQKMDALTSMSTVTGYKAVLLAANRLQ
                     KFVPMIGTAIGPVRPSRFLVVGAGVVGLQAIATAKRLGGMISAVDIRQKAREEATSLG
                     AAIGGFDVPGDLALGRGGYAKSLPAEWLEKEREALRPLMADADVVILSALVPGEVAPV
                     LVTDEMIASMKPGSVIVDVSIDQGGNCAVTEAGRHIVRHNVTIDGLQNIPGRVAIHST
                     WLYATNMYYYVENLFKGGDFRPDLEDEIVRSSLVTHEGRIVHQGTLKAMDQSN"
     gene            17397..17681
                     /locus_tag="PLZ75_02430"
     CDS             17397..17681
                     /locus_tag="PLZ75_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOS71286.1"
                     /translation="MSELLIMLAVFVLSIIIGYLLISKVPNMLHTPLMSMTNAISAVT
                     ILGALLVFAVKPDLTETLLGAAAIALAAFNVVGGFTITERMLRMFKSRKE"
     gene            17706..>18008
                     /locus_tag="PLZ75_02435"
     CDS             17706..>18008
                     /locus_tag="PLZ75_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOS71287.1"
                     /translation="MNSLTDLLIDLIIILIFLSGFSRFRTPGAARTGNLSAAFALALA
                     LVVVMARHRIMQPHVVIIALMIGSVLGWLVAMRVNMIQIPAMVAFQHGAGGIAAFLV"
BASE COUNT         4961 a         3864 c         4657 g         4526 t
ORIGIN      
        1 atcaaaatct gtgttaccgg cagcaacgga aagacgacgg tcacgaatct tatacggcac
       61 atgcttgaaa aagccggaaa aaaggttgcc atgacgggaa atgtgggaaa cagctttgca
      121 atgtcagtgg cacgggagca ttatgattat tatgttatag aactttccag cttccagctg
      181 gacgacatgt atgacttcag ggctgatata gccattcttc tcaatattac tcctgatcac
      241 ctggacaggt acggctatga tttcaggaat tatattgatt caaaattccg gataaccagg
      301 aatcagacga aatcggatta tttcatttac tgggcagatg atcctgtgat cacaaaagag
      361 ctggaaagaa gaaaatatga tatgacactt ctgccttttt ctgacaggca cacggagaaa
      421 atggcagcat atattgatca tgaagaatta attattgatt accagaataa aaccaccaag
      481 cttatgacca ttcatgaact ggcgctcaaa gggcgtcata acatttacaa ttccatggct
      541 gcagctattg ccggcagggt gcttgatata cgcaagcctg tgatcaggga gagtcttact
      601 gatttccagg ctgtggagca ccgtctggag ccggtgatta aggtgagtgg aataaatttc
      661 atcaatgact ccaaggccac caatgtcaat tccacctggt atgcgcttga atgcatggag
      721 actccggtgg tatggattgc cggcggaata gacaagggaa acgactattc cgaattgttt
      781 gaggttgtaa agcagaaggt aaaagcggtg gtctgccttg gaaaggacaa tgcaaaaatc
      841 attgatgctt tcaaagacaa ggtaaaaacc attattgaag ctgcatcaat ggaggaggcc
      901 gtccggtctt catactatct ggcaggaaag ggtgatacgg ttctgctctc gcctgcctgt
      961 gccagctttg atctgtttgt caactatgag gacagggggc gtcagttcaa ggctgccgta
     1021 agaaaccttt aatctgcagg gaaatgtttc agggatggct ttccagaacg tttaagggag
     1081 acagggcaat atggggtgtt gttgccgtct tcatggtcta ttcactgcta gccgtttaca
     1141 gttcctcagt gaatgtggct ttcaggaacc atggagggaa taccacatat ttcctgagaa
     1201 gccagttcct gatgctgact gcagggctgc tgataataat actggttcat tatctgccgt
     1261 acaggatata ttcggccctg gccgggattg ccgttatcgt ttcggcaata ctactcgtgc
     1321 ttacatactt ctttggcgtt acgatcaatg aagcgacacg ctggcttgag atcccctata
     1381 tcggtttcag gatccagacc tccgatattg ccaaggtagc cctggtcatt taccttgcaa
     1441 gatctcttgc cagatatcag aatcagctga aggattttgt gcttgttacc aaattcctga
     1501 tggtgccggt actgatcata tgtgcactga taatgcccga gaacctttcc acggcattaa
     1561 tggtctttgg aatctgcatc ctgataatgt tcattggcgg tgtcccggta aagtttctta
     1621 tagcatatgc gggaatggct gtggtttttg taatattttt tgcccttgtg ctctggctgc
     1681 tgaaagaaga caacagggtt cttacctgga ttgaaagaat gataagcttt tttgcgggaa
     1741 agagcagcgg tgctgatgat tatcagatca accaggctaa aattgccata tctacgggag
     1801 gcttgtttgg caaggcaccc ggtcacagca ttcagagaaa catgctgccg cagtcaaatt
     1861 ccgatttcat ttttgcaata ataattgagg aatacggatt gctgttagga gcaatcccgc
     1921 tgatcatggc atacatgatc cttcttttca ggggaatatc aatcgtgaaa aagtgcgaga
     1981 ccgcatttcc tgcctatctg gttctcggac ttatagtgat gatagtgaca caggcgatgc
     2041 tcaatatgat ggttgccgtt gatattttcc cggttacggg gcaaacactc ccgctggtca
     2101 gctggggacg gacatccgtg gtcatgatgt gcttttcgat aggggtaata ctgagtgtca
     2161 gcagggtggt gaatgccagg ctcaggaagg aagagcttcc cgaagaggag caaaaggatg
     2221 aaggtgaact gatttatgga acagttgcga aataaaagga ttatcatcag cggaggaggt
     2281 acgggaggac atattttccc ggctatttcc gttgccaatg ccctcaggcg tattgagccc
     2341 gggacggata tcctttttgt aggcgctgaa ggcaggatgg aaatggaaaa agtaccggca
     2401 gccggttaca ggatcagggc cttaccggtt tcgggatttt acaggcagat cactttcagg
     2461 aatttcagtg tcctgctgag gctgatcagg agcctttcca tggcaaaaag gataatcagg
     2521 gagttccggc cggatgtcgc ccttggcgtg ggaggctttg caagtggtcc ggtgatgtgg
     2581 aaagcggcag gaatgggaat accggtagtt atccaggaac agaacagtta tgcgggagtc
     2641 accaacaggc ttcttgcaaa aagggcttcg gccatctgcg tggcatatga cggcatggaa
     2701 aaatatttcc cccgtgacaa gatcatcaaa acaggaaatc cggtccgtca tagttttgac
     2761 aattccgggg ttcttaaaaa tgaggcactg gaatatttcg gtttcagcag ggaaaaacag
     2821 gttgtccttg tgctgggcgg atccctcggt gcaggcacta ttaataaaac cctcggcggg
     2881 gccctggaca gaatggccgg atcaggcctg caatggctgt ggcagacagg aatgaactac
     2941 tatgaggatg taaagagcct tgctgctgat tcacttgcgg aaaccatagc catccatggt
     3001 tttattgacc ggatggacta tgcatttgca gctgccgaca taattgtttc aagagccggt
     3061 gcaggaacca tttccgaact gtgccttgtg ggcaagccgg tgatccttgt tccttcgccg
     3121 aatgtggccg aagaccatca gaccagaaat gcagaagctc tttccggaaa tggagctgcc
     3181 attcttattg ctgacagtca ggcagagaag aaactgattg atgaggtcat caggctttct
     3241 cttgacactt ccgggaaagc aggactttcg gaaaatatca gaaaaatggc ggaaagggat
     3301 tccgatataa gaatagcgaa cgaagtattg aaactggcag ggacatgaat gatctgaaca
     3361 acatggaagg cgtttatttt gtaggtatag gcggtatagg gatgagtgcc cttgctctct
     3421 ggttcaggca caggggatat gttgttgcag gttttgaccg gactcaaagc agcattaccg
     3481 attcccttgc cgcgaaagga tgtgaaatag tgtttgagga tgatccctcc gcgctgcctg
     3541 agctgttcag ggaaaaaatc cccgggaagg agattcttgt ggtctatact cccgcaatac
     3601 cggcggagag cagcctgctc agccatttca gggagaaagg gttcaggctg aggaagaggg
     3661 cggaggtgct gggaaccatt tcacgcagca ccgataccat tgctgttgcc ggaacccacg
     3721 gcaaaacaac aacctcgacg atgaccgcac atttgctgaa gcagtcccgt gttgactgtt
     3781 ccgcattcct cgggggaatc tcacgtaact atgattccaa tctcctgctg ggtaacagca
     3841 ggttcaccgt gattgaggcc gatgaatatg accgttcatt tcaccagctt gatccgctta
     3901 tggcagtgat cacatctgtt gaggccgatc atcttgacat ttacggcgac ctgaaaacaa
     3961 tgacggaagc ctatgatgtt ttttgcggga agatcaggcc gggagggctt cttctgctga
     4021 accacaggat aagggacaga ctaaagcatc cggaagatgt atcagttttt acctacggta
     4081 atgatcccgg cgcggattac agatatttca atgttgtttc aggggaggat tattatctct
     4141 tcagcctttc cacgcctgaa ggaatgattg agaacctgcg tttccacttt cccggcagaa
     4201 taaatataga aaatcttacc gcggctatcg ccatagcgct taaatgcggg gtaagcgaaa
     4261 ccgaaatccg cagatcggta ccgcttttca agggcgtcag gaggaggttt gacatacggg
     4321 ttaacatgcc gggactgacc tatattgatg actatgccca ccatcccggg gaaataaagg
     4381 cctgtatcag atctctcagg gagtatttcg ggggaagaag gatcaccggg atctttcagc
     4441 ctcatctgtt ttccaggaca cgtgaccatg cggatgaatt tgcagaaatt cttgatgagc
     4501 tcgatgaggc aatacttctt ccgatttatc cggcaaggga aaaaccaatt gaaggagttt
     4561 cctcggaact catttttgaa aggatgaaac tggaaaacaa aaaaatgttg cagccgcaga
     4621 atgtggttga catgcttgat cccggcaggc ttgatgtcct tgtaaccata ggtgcgggcg
     4681 acattgacag gcttgttgat ccgattgaga ggaaactgcg gggtgaaaac ccggaaaaat
     4741 gaaacaacag aggaacagga aggaaatgaa aggatgataa tgaacagatg atgcgtctta
     4801 tgaaaatatt gctgattatt cccctgatct acatgctggc ggctccggtc tggttgtcgt
     4861 cctactacgg atcgaggccc tgcagggata tttccatagt catctgcgat tcatccgatt
     4921 atgattttgt cacaaggaaa cagcttctca gcctggttaa cggaagggag ggcagtctgc
     4981 ttggccgtcc tcagggcagc ctgccgctgt gggacattga gggaagggtt cgcacactga
     5041 aagaactgcg gaaggcagaa gcatatatga gcattgacgg cattctgaat gttgatgtat
     5101 accagcggaa tcccgtcatg cgggttattc ccgatgaggg aggggatttc ctcgttgatg
     5161 cggagggggt gctgatcagg aaaaggaccc ttcagagccc gaggctgcat atcgtcggcg
     5221 gggatattgc aataagccgt gcaatgcttg aaggtaccag tatcctggat gcgggacata
     5281 caatcctgaa ggatgcatat cattttgtca gatacatttc cggagatgat ttctggtcgg
     5341 cacagataga tcagatctac gttgacaagc gggggaagat agatcttata cccagggcgg
     5401 gaaaccaccg gatacacctg ggaacatttg aaaattttga aggaaagcta aaaaaccttg
     5461 cagcatttta tgaaaatgtt atcccggaaa cgggatggga taaatattcc gtgatcagtc
     5521 tggaattcag ggatcagata gtatgtaaga aaagacaaca ggcaaaataa aacaattgtt
     5581 atgagcaaga gagaacagta cattgcagca atcgacatcg ggaccaccaa aattgtttca
     5641 attgtcggca agaaaaatga aaacggaaag attgagatac tcgggctgag caaggctctc
     5701 tcaagggggg tgaaaagagg ggttgtgata aatattgaag agacggtcac ggctattcag
     5761 accactgtgg aggatgtgca gaaaaggtcg ggtgtgaaat tctccgaagt atttgtgggg
     5821 atagcgggtc agcatataaa aagcatgaaa agccgcggtt atattacccg tgactcctat
     5881 gaggatgaca taaaaaaaga ggaggttttc aggctgattg aggatatgca caagatccat
     5941 gttgaaatag gagaagagat aatccatgtc atcccgcaga atttcattgt tgacgatgaa
     6001 acaggcctga agagtcccat cggtatttgc gggcggaggc ttgaagccaa ttttcatatt
     6061 gtcataggcc aggtggcggc agcaaagaat attgaaaaat gcgtgaggcg tgccaacctc
     6121 agtgtgaagg atcttatcct tgaacctctt gcttcatccg atgctgttct cactgatgat
     6181 gaaaaggaag ccggggttgt tcttgttgat attggcggcg gcactacgga tatggctgtt
     6241 tattacgaca acataatcag gcacactgcg gttattcccc tgggcgggaa tgtggttacc
     6301 aatgatataa aggaaggctg tgccattctc cataggcatg ccgaacagct caagatacag
     6361 tacggatcag ccctgggtga tattgccccg gatgacaagg tcgttgccat tcctggcata
     6421 agcggaagga atcccaagga aatctccttc aagagtcttg cctatatcat ccagtcaaga
     6481 atggaagaaa taatagattt tgtcaatttc gaaatacaaa actccggcta tgctgaccgg
     6541 cttgctgccg gaatagtcat aaccggcggc ggcgccatgc tgaagcacct gccccagctc
     6601 atgcagttca ggacagcgat ggatgtccgt gtcggatatc ccaacgagca tcttggcgga
     6661 gcaggcaggg aagagataaa ccagcccatg tattcaacga gtgtcggact aataatgagg
     6721 ggctttgact ttctggagac ctacaggaaa tcgttcaatg cagcacctca gcatgagttt
     6781 acggaacagg gcggaggcag ctttgagcct gcatctgtgc cgggcgaaga tcaggcagac
     6841 actgctgcag agccgaaaac cagtctgacg gaaaaaataa aaatgctgat gcagaaaatg
     6901 tttgaagtgg aggacaatcc aataaatcaa tgattataaa ccctgaagtg taataaaatt
     6961 gaaggccgtg gaaaagaaaa tgatattaag tgaaaattcc gatgacctga taaatttcga
     7021 tcttccggtt gaaagatcat caataatcaa ggttgtggga gtgggcggcg gcggtgtcaa
     7081 tgccgttaat catatgtttg agaagggtat aaaagacgtt aatttcattg tctgcaatac
     7141 cgaccgccag tctctcgaca agagcccggt accggttaag atacagctgg gtgcttcgct
     7201 taccgagggc atgggtgcgg gaagccagcc tgaaaaaggc aggaaggctg ccgaggagaa
     7261 cattgctgat gtcatgaatg cactttcggg caataccagg atggttttcc taacaacggg
     7321 catgggagga ggaaccggga caggagcaat accggtaata gcaaaagcct gcagggaggc
     7381 cggtttgctt accatagcgg ttgttaccat cccgttcaag tcagagggaa agatcaggat
     7441 caactatgct gtggaaggca tagctgagct cgagaaccat gtggattccc ttctggtgat
     7501 aaacaacgaa aaactcaggg atatttacgg aaacctcggc gtgtcatctg catttggaaa
     7561 agcagatgac gttctggcca cggccgtaaa gggcattgca gaaataatca ctgtaacagg
     7621 atatatcaat gtcgactttg ccgacgttga aacagtaatg aaaaattcgg gagtcgctgt
     7681 catgggtatg ggaattgcag caggagagaa cagggcaata gaagcaatgg aaaatgcact
     7741 ggcctctcct ctgcttaatt caaatgacat aacaggggca aaaagcattc tgataaacat
     7801 atcctcggga agggccgagg atgaacttac aatggatgaa ctgggtgata ttacagatta
     7861 tatatattcg gttgcatcgg atgatgcact gataataaga ggactctcat cagatgagga
     7921 actgaaggaa aacctgagca taatagtcat tgccaccggc tttgaagcca acaatattat
     7981 tcagcccatg aagcccagga gggcaaaaaa ggttgaagtc ctgagttccg gtgactatcc
     8041 gctgaatccg ggaagagaaa cggaaaaagc tccggaggaa ttcagggttc acggaaaaga
     8101 aaatgaaccc ataattgcag attttcggga aaattaccag ggcaccctcg atttcagcgt
     8161 tgaaactgca aggggcagaa aatcatctgc tgacagggat aatgatcatg aaggcgggga
     8221 agatcccgaa ttgcgcctga agaaggtcaa gcacatgcag aaaatcatgc agaaggaggg
     8281 aatatcgagc aggaccatga aggagaacat tgaggcattc gaggatgtgc cggcctatat
     8341 aagaagaaat atgtcgctgc cggcccctga caggcagtca ggaagcgaat tgtcacaatt
     8401 cactctcagc tcggatgaga atgatggtcc ggtaatcagt aaaaacaatg cctatctgaa
     8461 cgataatgtg gattgacacg gaatccgcat cattcccggc ctgattaaca cagctgcggt
     8521 gttgtcatta ttcctatatt tgcgcagatg atcaggaatc tgaaaatatt gttcatttta
     8581 ctgattctga cgggatcagg cctttctgca cagaaatctg ccggaaccag aacgatcagg
     8641 caatggaatc tcagccacga cttcacggaa gagacaatca ttccatttga tacagtcttt
     8701 tccctgtttc accgttacag gattgctgac aggtattctc cactgaatgc caccctcgga
     8761 aattacggac ttccttttta ccagattaat ttttttgacc ggataactga tccggatgaa
     8821 ttcctgtatt cttattatct tcccgtgatg tatgttccgc gaaaggccct gttcatgaac
     8881 actcaggttc ccttcaccga gatggtctgg acaaacgggg ggccacggca gatgtccgaa
     8941 cagacattca gggtgcgcca ttcccagaat gtaaacagat atctaaattt cggactggta
     9001 tatgatattg ttttcaatct cggacagtac agctaccaga aggcagttga caagaacttt
     9061 acgttctttt cgtcatatac gggcccggtg tacaaatact atttctcagc cggcatcaat
     9121 aatataacgt cgtatgagaa cggcggaata aacgacaggg aggatctgga cctttttgac
     9181 acccgggatg tgccggtgag gatgggcggc ctgaacagtg cgaggagcgg tcttaagaac
     9241 aagaatatgc ttctggtgca gaggtatacg gtgggcagga aaaccggaac ggcagattcg
     9301 ctgaacagca gcagggattt ctccgggctc aggggcatat tttcccatat tttcattttt
     9361 gacaattcag gcagaacgta ttccgacaaa tatccgcaat cgggttttta tgatacaata
     9421 tatataaaca attccttcac ctatgattcc ctgtcgcagc ggattatcaa aaataccctg
     9481 agatttgact tcagcactga tgaaaaaagg aaattccgtg tcggtggcgg ggcgggcata
     9541 aggaatgaac tgatacgcta cagccagatc tgtccgctgc cggacacctt gtctgctgac
     9601 acgctggtct ggaggaagcc gaacaatgtc ctgaccggca ggctttacaa caacatcgga
     9661 gagaagttca tgtggacagc tgacggggaa cttttcctga ccggatacag ggccggggat
     9721 ttcaggctta agggggaact tgtaaaatcc tttgaatgga aaaagggcac ggctgtctgg
     9781 aatatcaacg gcagtatcat gaacaggcag ccatctttct ggtatgaacg ctgggggagc
     9841 aatcacttca gctggaatgc aggcatgaaa aaggaattca gagtagatat aggaagccgt
     9901 ttttcctatc cggccgcgaa aacagatctg agattcaact atgcaatcat tgacaattat
     9961 acggatttca atccggaatc acttccatcc cagcacggag gcggattgtc tgttttgtca
    10021 gtcatggcag gaaaggagct tacggcatgg aaaatgcacc tctccggcga tttgctgaca
    10081 cagctaagca gcaatccggc ggttcttgac cttcccctcc tctcggtacg tactgccggt
    10141 tatattgaac acctcttcag attcaggcag acaaacggtt ccctcaacag ccagttcggg
    10201 atagatcttc tttatcatac tccctatcgt gcatattcat acatgcctgc cacgggacgc
    10261 tttttcaggc aggaaaaaac aaaaaccgga aactatccct ttgtcaatat ctttctcaat
    10321 ctcaagctca aacgtacacg gatctttgtg atgctggatc atgtcaattc gggattatcc
    10381 gggagtgact atttcatgat tcccttatat ccaatgaatt acagaatgct aaggtatgga
    10441 attgcatgga cattttataa ttaaaaaaat gtattatctt tgccgcgttt ttgtctgaaa
    10501 atatttgttg aactaatgct tcatcattgg gaaaaataac aatctttatc acagccatac
    10561 tggcagcggg attaatctcc tgtacagatg gcaaaattca cccgccagaa acagatccca
    10621 gcaatctttc ctctgatctt gaaattataa ggcagagagg gaagctgatt gccataacgg
    10681 atataaactc aacgaattat tttgtttaca agggtgaacc gatgggcttt cactatgaac
    10741 tgctcaatga ctttgcagat tatctgggcc ttgaccttga gctgattact gaaaatcagc
    10801 ttgacaaagc ctgtgatctt ctgcaggacg ggaaagctga cataattgca attgggctga
    10861 cagttaattc agccaggaag aaaaaaatac ttttcactga accgatactg cagacgcgtc
    10921 aggttcttat ccagcgcaag ccacggaact ggaggtccct tacggccgct gcccttgaaa
    10981 ggcagctggt gaggaatcag cttgacctcg caaacaagac ggtttttgtt caggagaatt
    11041 cggcgtacat taaccgtctc agggcacttt caaatgaaat aggcgacacc atagggattg
    11101 ttgaagttcc ctataattca gaagagctga tcaggtttgt ggcaaacggg gaaatagaat
    11161 ataccataag cgatgagaat gttgccatgg ttaattcaac ttattatcct gatattgata
    11221 tcagcacacc ggtaagcttt cctcagaata ttgcatgggg tctgaaaaag cgtgaatctt
    11281 ccgatctgct taacgaattg aatcactggc tttccaccta taaggagacg aggaggtacc
    11341 gggcgatgta tgccaaatac ttcaggaaca gcagatccaa ttacatggtg aagagcgatt
    11401 actattcact gagcacgggc aagatctcca ggtgggatga tatgatcaag agggcaagcc
    11461 gggaaatcga atgggactgg cgtcttctgg cctcgctcat ttaccaggag tcaaggtttg
    11521 atccggatgt tgagtcatgg gccggagcct acggacttat gcaggtgatg cctcccacgg
    11581 gcgaaaaatt cggaatagat gtaaaatcat ctcccgcaag caacatgagg gctggcatat
    11641 tgtatatcaa ctggcttaag acgatgttcg agaataagat tcccgaggag aacgaaaggc
    11701 ttaaatttat tcttgcatcc tataatgccg gacccggaca tgtcctcgat gcaatgaaac
    11761 ttgctgaaaa gaacggaatg gatccccgca aatgggaaga caatgttgca gtgtggctgc
    11821 tgaaaaagtc ggaccctgtt tattacaagg atgcagttgt aaagaacggc tatttcaagg
    11881 gcacggaatc ggtcaactat gtaacccaga tcctggaaag atatgaacat tacaagaata
    11941 taatttccgg ggaaaacagc attcgcctga cgatgaacac agatcaggac agcagggata
    12001 attccctcag aatcagtaac tgattcattt ctctagtcgt atcttatcgc tttcacgggt
    12061 gagatcctgc ttatcagctt tgacggcacc aggagcatca cgagtatggc ggccatggtg
    12121 ccggcattga gaaaaactat gtggatcatc tgcagattga ccgggacggt tttgatataa
    12181 tacgaggagg gatcaagaga tatcaggccg gtttttaact gcaggactga aagccctata
    12241 ccgatgatgt ttccccagaa caggcccttg ccgatcaggt aggctgcctg gtagagaaag
    12301 acccccctga tggttttgtc gtgagaaccc agcgccttca gtattccgat catgtttgtt
    12361 ttctcaagta tcaggatcag caggttggat accatattga aacctgcaac ggtaagcatc
    12421 agcagtatta ttattatcac attgatatcc tgaaaattga gccagtcaaa gatctgcggg
    12481 tatttttccc ttatgctgat cacattgaac ttttcttcgt cacggagaag ctgataaccg
    12541 attgcagacc ttaccgctga tgtcatttgg tcgagttttc cgaaatcatt gatgaagatc
    12601 tcaaaaccgc tcacctggtc atcatcccat ccgttaagac gttttatgtg gtttatgtcg
    12661 cagaagacat agattttgtc aaactcctca aggctggtct cataaatgcc gctgatgaca
    12721 aatttcctca ttcttggcgg gtcctgaaca aaatgcatgg caaacgaatc acctgtcctg
    12781 agccggagca tatcggatat ttttcttgat attatgaccc tgtcggtccg tgttgaatcg
    12841 ctcacccgga aaacctcccc ctcaaccatg ctggaggcaa aatagctcca gtcataatca
    12901 gaacccactc ccttgagcac aacgccctga atattgtccc cggtttttat gattccggct
    12961 tttgttgcaa aaacctggac gtgttttatt ccgggcaggt ttctgatttt tggtatgaat
    13021 tcctggccct ggcggatggg tacagtctca aaggacaggt ttgaatcaag gttcacaatc
    13081 tggatgctgg atccgaaacc tatgatcttt tcccttatct gctgcttgaa gccggtcagg
    13141 atggccactg caagtatcat gaccgccagg cccgtggcaa tacctgcaat tgcaattaca
    13201 ttgatgggcc ttgaaaatga tgtgccctcc ctgcgtcccc tgataagacg ttctgctatg
    13261 aaataaggta gattcatctg tcaaatgaat tcaaaattaa taaaattaac acaaccggtg
    13321 cgggtcggga tttcatcagg gaaaaaattg taaattaagc gccgttaagg cgtctgtatg
    13381 gaagttgtcc ggcgcagcct ccattcaggg ggagcgggaa gagattccgt taccggcaga
    13441 ggggaggacc gggcaacagg ccgggaacct gtggcaatgg acagggcccg gtatgcagga
    13501 caggatctgc cgggccagga ggcaggatta ttaatgggaa tcagaataaa attacttgca
    13561 tatgaaatac ttactgacat ttctgttgat ggttgcttct gcatgcgcat gcaaaagcgg
    13621 taacgaaggc cctgtgcccg gagcggagag accggaggaa tacatgaaaa tgctggatgg
    13681 tctggctgtg ggaattgtgg ctaatcagac ttctatggcc ggggagaccc atctggttga
    13741 atatttactt ggcaggggag ttgatgtgag gatgattttt gctcccgagc atggttaccg
    13801 ggagctcgcg gcggatggtg cgcacatagg cgacggaaca gatgctgcca ccggaattcc
    13861 ggtcaggagc ctctacgggg tccgccgcaa accggacccg gaagacatgg ccggactgga
    13921 tgcggtgatt tatgatattc aggacgtggg agcgcgcttt tatacctata tatcaactct
    13981 tcattacgta atggaagcct gtgctgaaaa caatgtgaaa tgcattgttt ttgaccggcc
    14041 caatcccaac gggttttatt tcgacggtaa tattgccgac actgcctacc gttcatttgt
    14101 ggccatgcac ccggtcccgg taatgcacgg aatgaccatc ggggaatatg cggggatgat
    14161 aaacggtgaa aaatggcttc cgggcggaca gcagtgtgag ctcgcagtaa taaggtgcag
    14221 aaattacacc catcagtccc tgtatgaact tcccgtaagg ccttcgccca accttccgaa
    14281 catgaccgcc gtttatcttt atccctctct gtgtttcttc gagggaacaa gtatttctct
    14341 cggaaggggc actccctttc ccttccaggt ttacgggtcg ccgctgtttc ctgatgaagg
    14401 gttcagcttt acgcccgaaa gtgttcccgc atcagtcaga cctcccctgc tgggagtgaa
    14461 gtgttacggg gttgatctga ggaatgcgat ggaggaaaaa cttgtgcccc ggcccatggt
    14521 caatcttgac tggctgacgg atgcctggac gaaatatccc gaaaaggaca aattctttaa
    14581 caattacttt gatgttctgg cagccggccc gacactgagg gaacagattc agtcggggat
    14641 gagtgcggca gagatccgcg ccacatggaa ggacggactg gaaaagttcg ggaagacccg
    14701 gcaaaaatac ctgatctacg aataatcccc tgaatcattt gctgctgccg tctccctgtg
    14761 ttcttcccgg ataaaccttc agtgcctttt ccaggaccat tgcggcattc ctgagatccc
    14821 cgatattcag cacataggca atccttgcct cattcctgcc cagtccggga gtggaataga
    14881 atcctgatcc cggtgcaatc atgacggtct gcttttcgta ttcaaattct tccagcagcc
    14941 attgtgcaaa tttgtcggca tcatcaaccg gaagtcttgc catggtataa aatgcgccct
    15001 tgggcatcgg gcaacgcacg cccggtattc tgttcagggc atcgacaatg aaattccggc
    15061 gggccaggta ttccctgttg acttccgtga aatactcagg aggtgtgtct attgaagctt
    15121 ctgcgactat ctgcccgaga ccggggggag aaagacgcga ctggccatat ctgaggactg
    15181 tcgacatcac ctctttgttc ctcgagataa ggactcctat ccggattccg cattcactgt
    15241 aacgcttgga tacagaatca atcagaatta cattttcttc tatccccttg agattcatgg
    15301 cagaaactac cttctggccg tcataggcga attcacggta agcctcatcg gaaaaaagga
    15361 agaggttgta tttttttacc agctccctta tctggtccag ctcgccggcg gaatagacat
    15421 atcccgtagg gttattggga ttgcagagca ggatggccct cgttttctca ctgatctttt
    15481 tttctatttc ggaaaccggg ggcagggcaa aatccacctc aatccctgaa gtaactgaaa
    15541 caatccttac tcctgcaatg gaagcgagag cattgtaatt tgcatagaaa ggttcaggaa
    15601 ttattatttc atctccgtag tcaaggcatg aattgactgc aaacgaaatt gcttctgagg
    15661 cccctgttgt aataagtgtc tggtcccagg aaatatctat tcccgcattc ctgtaaaaac
    15721 cggccagttt gcgccggtag gattcataac cggcggaatg actgtattcc aatactttct
    15781 tgtccaggtt tcgcatggca ttcagtgcga cctcaggagt gtgtatatcg ggctggccga
    15841 tattgagatg atacaccttt attccccttt taacggctcc ctcggcataa ggaatcagct
    15901 tccttatcgg tgaggcgggt atatttttac tgcgttgtga gagtaatggc atgttttcag
    15961 aagtttatgt atgtgtagaa ccttttatac aaaggtataa aatattaggc agtaaacatt
    16021 taatactttc cagtatttgg aacggtgcat cagcttttct gtttttagtc ctatttgccg
    16081 gaatgcaaat tatttagcgg aaattcttcc ggatttctaa aaaatgaacg atatttaagt
    16141 tacatttagt tacaggtttt cctgatcgca gacctgatat aaaaaaagtt taatcaacat
    16201 gaaaggaggt ttgttatgtt aatcgctatt ccaaaagaaa ttatggaaga agaaaagaga
    16261 gttgcagcaa ctccggagac aacagagaag cttataaaat taggttttaa ggttatccta
    16321 gaatcaaacg ccggcgcagg tattttttac agcgatgaag attacagatc ggccggggcc
    16381 gagattgttg ccgatgcaga gactttatac ggcagggcag atatcattct taaggtcaag
    16441 gagcccagat acaatgtgaa tgccgggaaa catgaagttg agatgatgaa ggatgaaagc
    16501 atactgatcg cctttcttca tccggcaaca ccatccaatc attcattggt taagatgttg
    16561 agagataaaa aaataacatc tctgaccatg gatggtattc ccaggactcc gaatgcacag
    16621 aagatggatg ccctgacttc aatgagcacg gttaccggtt acaaggcagt gcttcttgca
    16681 gccaacaggc ttcagaaatt tgttccgatg atcgggacag ccataggtcc tgtaaggcct
    16741 tccagattcc ttgtggtcgg ggccggtgtg gtcgggctcc aggctatagc cacggcaaaa
    16801 aggctcggag gcatgatcag cgctgtggat atacggcaga aggcaaggga ggaagcaaca
    16861 agcctgggag ccgcaatagg cggttttgat gttccgggcg atctggccct gggcaggggg
    16921 ggatatgcaa aaagcctgcc ggccgaatgg cttgaaaaag aaagagaggc actcaggccc
    16981 ctgatggcgg atgccgatgt tgtgatacta agcgcacttg ttccggggga agttgcaccg
    17041 gtcctggtga ccgatgaaat gatagcctcc atgaaacccg gatcggttat agtcgatgtg
    17101 tcgattgatc aggggggtaa ctgtgccgtt acagaagcag gcaggcatat tgtaaggcat
    17161 aatgtgacca ttgacgggct gcagaatatc ccgggcaggg tggccataca ttcaacctgg
    17221 ctctatgcga caaatatgta ctattacgtt gaaaacctct ttaagggagg tgatttcaga
    17281 cctgatctcg aagatgagat tgtcaggagt tccctggtta cccatgaagg aaggattgta
    17341 caccagggga cactcaaggc aatggaccaa agcaactgaa acccaacaag acgaatatga
    17401 gtgaattact gataatgctc gcagtatttg tactgtcgat aatcatagga tacctgctca
    17461 tctcaaaggt tcccaatatg ctccatactc ccctgatgtc gatgaccaat gccatttcgg
    17521 cggtaacgat acttggagcc cttcttgtct ttgcagtgaa gcctgatttg acggaaacac
    17581 tgcttggtgc agcagcaatt gccctggcag cattcaatgt ggtcggcgga tttaccatta
    17641 ccgaaagaat gctcaggatg tttaaaagca ggaaagaata acaacaggaa cttaagctga
    17701 agcagatgaa cagcctgact gatttattga ttgatctgat aatcattctt atatttctga
    17761 gtggtttttc aaggttcaga acaccgggag ctgcacgaac aggtaatctg agtgcggctt
    17821 ttgcccttgc gcttgctctt gtcgtggtaa tggcccggca caggatcatg cagccccatg
    17881 ttgtgatcat tgccctgatg attggttcgg ttctgggctg gctggttgcc atgagggtga
    17941 acatgatcca gattcctgca atggtggctt ttcagcatgg ggcaggcggt attgcggcat
    18001 tccttgta
//
LOCUS       DAOSPX010000025        17939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3266_length_17939_cov_4.120555, whole genome shotgun sequence.
ACCESSION   DAOSPX010000025 DAOSPX010000000
VERSION     DAOSPX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17939
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3266_length_17939_cov_4.120555"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..2052)
                     /locus_tag="PLZ75_02440"
     CDS             complement(43..2052)
                     /locus_tag="PLZ75_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71288.1"
                     /translation="MKRSGIILIILVFTFTDQVLAQKIDSLTFSLNTRYSFYSFEDNA
                     EIILHIPPAHTSDRLAVLVTLANGDTVCTWSGTARNRLVRIPFKITTGKGFHRLNAEI
                     HAAGTTTRFIAKTLLLHLDYKPNEVKTDRLTGGLIVNKRQFFPAGFYCYSPVHPSLPE
                     EEAVRGFNMISPYQKILPSSFPERKAYMDRCAQLGMKVHYNLLSVAGGGGVASQLEGI
                     TAAGKKALLLNEIKSFRDHPALLAWYIADEPNGYRIPPDTIRAIYELIRENDPWHPVS
                     VVFMAPFTDARKYINALDIVMADPYPIPVSPVTLPGNVAKTLSNEFRGRRPVWMVPQA
                     FGGGELWSREPTVQEVRAMTYQSIINGAKGIQYFVREGLNLFPKSTALWNECGKMAVE
                     IAEMTPWLLSDEASVAVSSGSENIMLASFAHRGQLMILAANRTNMPVRADFSLASPLS
                     GRARVIFENRFVTLKDGKFNDQLSALGTQVYLFNLLTVKENVKPFKGNLITDPGFEDL
                     SSPGIPASCYAWNNGDRGATFFLDSREHVEGNHSLRLITPADNRSARLRFFPFRTSPG
                     KTYYISVMAKTDPEIITDNPVTGSPLYFEISLGNYGSRRFALSSEWKEYVAAITIPYG
                     NNIPPKTNVILQLPSAGVAWFDMLQAIEGVDVNRSINPELLFIRE"
     gene            2242..5385
                     /locus_tag="PLZ75_02445"
     CDS             2242..5385
                     /locus_tag="PLZ75_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS71289.1"
                     /translation="MKKKMKSELFFPSGKRIGNYIAVTVLLLMILILIPVVKVSAVDH
                     FKILPDDFPVQNIVTGKVTDAKGESLPGVTVAVKGTNLGTLTDIGGRFSISNIPDDAV
                     LVFSFVGLKTLEISVAGQASIDVSMEEEAIGLAEVVVIGYGTALRKDFTGTVGSLNVE
                     SSPVAQLPKFNALESLKGSISGLNIGSTNTAGGQPSMLIRGQNSISGSNDPLIVLDGV
                     IYLGSLGDINPNDIASFDVLKDAVSAAAYGSRSANGVIAITTKKGLVGKPLISFKASS
                     GIQTWQNMPVMMKGEEWITVVNARNQYTPGSTEWMKPGELANLKAGKETIWLEEVTRT
                     GVIQDYQIAVSGANPNVNYYFSSSYDVNKGIIVGDDFNRLSFLAKLKTNVAKWLEIGL
                     DAGFSRRDYSGFSANIGTAQVMSPYGVMYRDDKGNLEKYPYTQSGVNPLWGVINDVAD
                     NKDIRNNFRLNAYSVINIPWVKGLSYRLNLQTNLDKNESGNFYFEDYYIAEGEGLERY
                     SAAVIQGFLSRANGNIDNNSTYSYVFDNILNYKHVHGDHSVDVTAVATRDFRQYKQVN
                     STGSDFAANGNTTLGRWGLHKATVQKVLLNAEDRANIGYYGRLNYSFGDRYFFTGSYR
                     RDGASVFGANNKWANFFAAGTAYKISNEPFFNIDLINSLKLKLSWGQNGNQGISPYGT
                     LSTVANAASGGSRYEFSNTGSTIYYGLYQSALGNADLGWETTESWNAGFESEILNNRI
                     SVDMDVYFSKTTDQIFTRNIPAMTGFNTIKTSMGQVNNTGIEFRISSVNIKKSDWSWN
                     SSFTFWKNNNKLVHLYGDDLDGDGKEDDDIANSLFIGKSLGAIYGYEQDGIVQENDAE
                     YIALTGAAPGAPKYKDHDKVPGITANDRIILGYTKENFRSSLSNIAGYRNFELYVMIM
                     GIFGGNNHYLKSNTAAYLTSGTGLFNANMTSKPYWTPENKSNLYPSATFAGDGRYLAL
                     QSRGFVRIQDVSLSYTFNKRLTDVLKVNSLKAYLSVQNMATFTNWFGGDPETGTTVRE
                     NTYPVPSTFSVGINATF"
     gene            5405..7189
                     /locus_tag="PLZ75_02450"
     CDS             5405..7189
                     /locus_tag="PLZ75_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS71290.1"
                     /translation="MNKINFIKHIWILILIFALFACESDKEFLNEKPTTFYTTDNAFS
                     TSAQIDQVLVGIYSQLRDLWANPGEAAWIFVFRGNGTDMFDVPSIRRGNTFNNYGTIN
                     PDHSHFLNIYSAWYQIISKANLVLYASELPQITWSSPAEKAYVLAQARFFRAFAYRNL
                     GELFGGVPIVTEISKTAKYDFERASRLETYQFAIDELLAIENDIPVTNVSGGRIVRGA
                     AQHNLCELYLALGTQLAADGNSSGAQTAFTNSISYANKVIDGGIYSLINNRFGKRKNE
                     LTINIPVYSQGNTSTPVDNLEQTTNHYWDMFQEGNVNYQDGNTECIWALQTDYAAYKA
                     EDGKSKLPYSRTYGPVFRDGAKNHLTGTLEDVGGRGISQVIPTMYTRDLIYEDKWAED
                     LRNSDVVFRRTFIGNVVTSPYYRIVVPWDIMYYESADPSTNNNNRSLCYPVSCKIATD
                     KYTGLDAGENRSNLFRDDYFIRLSETILLRAEAKQRSGDKNGAAEDINLLRNRAQCTY
                     LVTAADMDDKFNLILDERARELVYEECRWNTLLRMGGTIAVDRIAQYAYWPEAQATLT
                     FDYNLWPIPQTVIDTNKDKVLEQNPGWK"
     gene            7186..10317
                     /locus_tag="PLZ75_02455"
     CDS             7186..10317
                     /locus_tag="PLZ75_02455"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS71291.1"
                     /translation="MIRIFSGRLVPFLILSLISGQACKTTVTTDGFRKEISNDLRADF
                     LNPPSYARLRTFWWWLNSNVTKECITQDLEAMKANGYGGAIIFDAGSSNYTVAKKTDA
                     GPAFLSDKWMELYGHAIKEADRLGLELTINVQSGWNPGGPSVTPEFAMKKITWSETDL
                     SGPSKINSALPDPTSQLFYKDILVQAVRKFSGDQDSPGIKNHAIKTLLERIGWSGIYP
                     LHKLREDYDTEPLHTLNKGDIIDVTPFYKDGILTWDVPDGEWTIIRYGMTCTGVKVST
                     SSDGWDGLSMDHLNRNALTRYNEDVISRLIARAKQEGNSLKFIHTDSWEMGVANWTQG
                     FMEKFRELRGYDMTEYMPVITNRIVGNRDISNRFLHDFRRTVSDLIADEFYKTFSEIA
                     RQNDLFTHPESGGPHSAPIDAIKTMGYNDVPMGEFWVRSNTHRVSDAQRLCVKQSASV
                     AHVYGKQFVAAEGPTSIGPHWERPPRDCKNVIDRIFCSGVNRIVWHTFTASPDEYGEP
                     GNEYFAGTHLNRHVTWWKESEEFIRYMNRCSFLLSQGKFVADALYYYGDDTPNFIFIK
                     EEVTDLGQGYDWDKCSLDVLLNRVRIKDGKIILPDGMTYRILVLPEEKALNIKLLRKL
                     EKLVHDGLILIGPKPEKSTGLTEYPEVDAAVSEIAEKLWGNIDGNGIKVNKFGKGKVI
                     WGLTPGETLRSVNINPDFSYSSAADSTCLEYIHRSTGKEEIYFVVNRLARHGIYDTQY
                     RYLTDLPDRYEEVVCKFRVTGKIPELWNPMTGEIKQPAVYHDDGYYTYVPLHLNPEGS
                     VFVIFVANSNDKHIIKIVKDGEEIFPAAVRPGSKFPTIECYYTEEGVITTFSQPGNYT
                     LRWSDGTENTTNVKEPLSVIPIDSSINLKFLNQWGPKETIAISEFKSWSDFEDKRIKY
                     YSGPAEYLTVFNLKNGSLAGKKVLIDLGNVQEIAAVWINDEYAGVAWIAPFILDITGF
                     VHEGNNKLTVRVVNSWVNRLIGDGHLPPEKRYTRTNVLKFEGGDKDHLLRKSGLTDDI
                     KLIIIDEKIIGHF"
     gene            complement(10489..11472)
                     /locus_tag="PLZ75_02460"
     CDS             complement(10489..11472)
                     /locus_tag="PLZ75_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOS71292.1"
                     /translation="MKEQDVEITNSILFEFQRYVRGEMTKREEFTFQKNIRKDQYADA
                     AIVGFADVPEEEIPFFTNFAEEEEEKRIKSKKRLIYSIIASVIILLIATSAYLVAEKK
                     LSVKQFKPFRFLHSSLPAAPVVLPVIKDNQQIAAFQQAESMASLAIPDIRTLPPPPEI
                     IPDPEILPLAGDSFSGDNSELLATDNVTDSSAIQDQTVLTARAGSGDSPDIIAGSTAA
                     GEESAGQAGNDQGGYIAPQPVNGDNSFSKYIEENIRKPQTLPEGENAVAVVSFVVKTS
                     GDVDGIKVLSSPGEEYTAEAIRLIREGPAWKPAENNGMAVEDQKMLRIVFK"
     gene            complement(11846..12004)
                     /locus_tag="PLZ75_02465"
     CDS             complement(11846..12004)
                     /locus_tag="PLZ75_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71293.1"
                     /translation="MKRQSRITAVPHRALQKRIKRYGAVEINGQKIICSKQEFFGKRL
                     IRLSMLKL"
     gene            12156..13682
                     /gene="zwf"
                     /locus_tag="PLZ75_02470"
     CDS             12156..13682
                     /gene="zwf"
                     /locus_tag="PLZ75_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOS71294.1"
                     /translation="MEHPDNLILVIFGASGDLTSRKLIPALFSLKSQKLLPSQFAIVG
                     SGRTSFSGDEFREKMRTAIFAVHGGRNPDSRLVREFLEQIYYVTMDSAEPENYSRLKG
                     KLSLLNSECGTCGNYIFYLATPPVLYEVISTNLTKAGLADQSEGFRRFIIEKPFGYDL
                     ASAQKLTGKLHELISEDQIYRIDHYLGKETVQNILVTRFANGIFEPLWNRNYVHRIEI
                     TSSENLGVGERGGYYDTSGALRDMVQNHLLQMVGLTAMEPPSSMMPDAIRNEILKVFQ
                     SFQPLREEDVPHQVIRGQYLESRISGERIPAYRSEKGVSPDSRTETYVALKFFVNNWR
                     WGGVPFYIRTGKRLPVKVTEIVIHFKRTPHHLFQRNEGPMNSNKLILRIQPDEGILLN
                     FGMKEPGSGFNVKNVDMDFRYSSLADVRVPAAYERLLFDVMVGDSTLFSRDDVVETAW
                     RFLEPVQNAWKNDPGIKLYGYPAGTWGPDVADNLVEGCGLTWRYPCNNLAEDGKFCEL
                     "
     gene            13729..14466
                     /gene="pgl"
                     /locus_tag="PLZ75_02475"
     CDS             13729..14466
                     /gene="pgl"
                     /locus_tag="PLZ75_02475"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOS71295.1"
                     /translation="MEKTVRIYDSPEELAEAFARELASSFRNATEKGSVFTVALSGGS
                     TPSALYSLLAEKYADSVNWKYIHFFWGDERCVPPDDPESNYGMAFDKFLGRIDIPPGN
                     IHRIAGEGIPSVEALRYSGEILLNTRSFNGLPVFNHFILGMGEDGHIVSIFPSNREMF
                     HSDKICDVAVHPVTGQKRITVTGKVINNSESVTFLVTGKSKAETVERIFMKDNGFPAS
                     DVMPYHGRLAWLLDKEAASKISGNGQC"
     gene            complement(14714..14908)
                     /locus_tag="PLZ75_02480"
     CDS             complement(14714..14908)
                     /locus_tag="PLZ75_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOS71296.1"
                     /translation="MNKGKVKFFNVSKGFGFIKDADSDNEYFVHASGLVDKIKENDDV
                     TFELAEGKKGINAVNVKKIQ"
     gene            15054..16946
                     /gene="speA"
                     /locus_tag="PLZ75_02485"
     CDS             15054..16946
                     /gene="speA"
                     /locus_tag="PLZ75_02485"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOS71297.1"
                     /translation="MRKWRIEDSAELYNINGWGVGYFSINEKGNVIVTPQENNPGVDL
                     KELIDGLSLRDVTTPVLLRFPDILDDRIVNITNCFRKASEEYDYKAQNFIIYPIKVNQ
                     MRPVVEEIVSHGKKFNIGLEAGSKPELHAVIAINTEAESLIICNGYKDEDYIELALLA
                     QKMGKMIFIVVEKLSELKLITSIARRLKVKPNIGIRIKLASTGSGKWEESGGDVSKFG
                     LTSSELLEAIDFIEKNRLKSSLRLIHFHIGSQVTKIRRIKTALREASQFYVQLRKLGF
                     NVEFVDIGGGLGVDYDGTGSANSASSINYTLQEYVNDSVSTFVDASNKNNIPHPNVIT
                     ESGRSLTAHHSVLVFEVLETATLPEWDVEEEIGENDHELVKEIYTLWDNFNQQRMIES
                     WHDAQQIREEALDRFSLGIIDLRTRAQIEKLFWSIARKVYQTAGKSRHVPEEIRQISK
                     ILSDKYFCNFSLFQSLPDAWAIDQIFPVMPIQRLNEQPVVAATIQDMTCDSDGKIDNF
                     IATSNNSHFLPVHHLKPKEPYYLGVFLVGAYQEILGDLHNLFGDTNAVHVSVDKDGYS
                     IDQVIDGETIAEVLDYVQYNPKKMVRTVESWVTSSVKAGIITLEEGREFLSNYRSGLY
                     GYTYLE"
     gene            17042..17695
                     /locus_tag="PLZ75_02490"
     CDS             17042..17695
                     /locus_tag="PLZ75_02490"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOS71298.1"
                     /translation="MSNSSFDFNALIRDSKEILKDPKAYFSSMKTTGGIAEPVIKAVV
                     YGLVAGIFAFIWSLLKIGPASYGISGGAVGFMALVWYVVGAVIGLFIGAVILLVISAI
                     CKGSTDFESNVRVTAALMVMMPISAFLGFFMGIGITLGTIVSLIVSLYGLYLLYHGLI
                     ETLKANAGTSRVLVLVLAALLVIFSLVGIGARKKAASFMGDFNMDQIREFYEDAGNN"
BASE COUNT         5203 a         3547 c         4084 g         5105 t
ORIGIN      
        1 ttgctttgca ggaattaagc ccggaacagg gaccgggatt tgttattccc ggataaacaa
       61 cagttcagga ttgatactcc tgttgacatc cactccctcg attgcctgta gcatgtcaaa
      121 ccaggcaact cctgcagaag gcagttgcaa tataacattt gttttcggag gaatattgtt
      181 tccgtacgga attgtgattg ctgccacata ttctttccat tcactgctca gggcgaaccg
      241 cctgctgcca tagttgccca gggaaatttc aaaataaagc ggactgcctg ttacgggatt
      301 gtccgttatt atttccggat cggttttcgc cattacggaa atataatatg tttttcccgg
      361 gctggtcctg aaggggaaaa atctcagacg tgcgctgcgg ttgtccgcag gggttataag
      421 tctgagggaa tgatttccct ccacatgttc acggctgtca aggaagaaag tagcacccct
      481 gtcgccattg ttccaggcgt agcatgatgc aggaatacca ggactggaga gatcctcgaa
      541 tcccggatcc gttataaggt ttcctttaaa tggttttacg ttttctttga ccgttaaaag
      601 attaaaaagg taaacctggg tgcccagggc agaaagctga tcattgaatt ttccgtcctt
      661 cagtgtaaca aatctgtttt caaagatgac cctggctctg cccgacaatg gggatgcaag
      721 ggaaaaatct gcacggaccg gcatatttgt ccggttggcg gcaaggatca tcagctggcc
      781 cctgtgagcg aatgatgcaa gcatgatatt ttccgagccg gatgagaccg caacacttgc
      841 ttcatcagac aaaagccatg gtgtcatttc ggctatttca acagccattt ttccgcattc
      901 attccacagt gcggttgatt tcgggaaaag gttcaatccc tcccttacga aatactgaat
      961 accttttgcg ccatttatta ttgactggta agtcattgcc ctgacttcct gaacagttgg
     1021 ttcccggctc cataattcac cgcccccgaa tgcctggggc accatccata ccggccgcct
     1081 gcccctgaat tcgtttgaaa gggtttttgc cacattcccc ggcaatgtta caggtgagac
     1141 cggaatggga taggggtctg ccataacgat atcgagtgcg ttgatatatt tgcgtgcatc
     1201 cgtgaaggga gccataaata ctacagatac aggatgccag ggatcatttt cccggatcag
     1261 ttcatagatg gcccggattg tatcgggagg gatcctgtag ccgtttggtt catccgcgat
     1321 ataccaggca aggagggcgg gatggtcacg aaatgatttt atctcattca gcaacaaggc
     1381 tttcttcccc gctgccgtga ttccttccag ctgtgaggcc actcccccgc ctccggctac
     1441 ggaaagaagg ttgtaatgca ctttcattcc cagttgtgca catctgtcca tatatgcctt
     1501 tctttccgga aatgaagatg gcagaatctt ctggtagggc gatatcatgt tgaatcccct
     1561 gactgcttct tcttccggca gggagggatg aacgggagaa tagcagtaaa accctgccgg
     1621 aaagaattgt cttttattca caatcagtcc gccggtcagc ctgtcggttt tgacttcatt
     1681 gggtttgtag tccagatgca gcagaagcgt ttttgcaatg aaccttgtgg tagtgcctgc
     1741 tgcatggatt tctgcattca gtctgtggaa acctttcccg gttgtaattt taaagggtat
     1801 ccttaccagt ctgttccgtg cagtcccgct ccatgtgcag actgtgtccc catttgccag
     1861 agtgacaagc accgccagcc tgtctgatgt atgagccggg gggatatgaa gtataatttc
     1921 agcattgtcc tcaaagctgt agaaagagta tcttgtattg agactgaatg ttagtgaatc
     1981 aatcttttgc gccaggacct gatctgtaaa tgtaaaaaca agtattatta agattattcc
     2041 agaccttttc acgggatata ttattttaat aatattcagc ttttaaaatt attaattcct
     2101 cagaaattgt atttttgtta acgtagaagt tatcaataga tgttcacata acacaatccg
     2161 gattatcaaa catccggtac taaattcaga aatactgtaa cggtttttta atctttaatc
     2221 tttatttaaa tctgttaatc tatgaaaaag aaaatgaaga gtgaattatt tttcccttcc
     2281 ggaaaaagaa ttggaaacta catagcggta acggttttgt tattaatgat tctgattctg
     2341 attcctgttg tgaaggtttc agctgttgat cattttaaaa tacttcctga tgattttcct
     2401 gtgcaaaata ttgttacagg gaaagttact gatgccaagg gcgaatcatt gcctggagta
     2461 actgttgccg tgaaaggtac gaatcttggt acacttaccg atatcggagg caggttttcc
     2521 atcagcaata tacctgatga tgctgtactt gttttttcat ttgtcggatt aaaaacactg
     2581 gaaatatctg ttgccggtca ggcctcaata gatgtcagta tggaggaaga agctatcggt
     2641 cttgctgaag ttgttgtgat cggatatggg acagctttgc gaaaggattt taccggaacg
     2701 gtcggttctc taaatgtaga aagctcgccc gtggcacagc ttccgaaatt caatgcactt
     2761 gaaagcctta aggggagtat ttcaggcctg aatattggat caaccaatac tgcaggagga
     2821 cagccaagta tgctgatccg tggtcagaat tcaatttcag gttcaaacga tcccctgatc
     2881 gtacttgacg gggttattta tcttggcagt cttggggata ttaatccaaa tgatattgcc
     2941 agctttgatg ttctcaagga tgctgtttcc gcagctgctt atggttcaag atcagcaaac
     3001 ggggtaattg ccattacaac aaagaaagga ctggtaggaa aaccgctgat aagttttaaa
     3061 gcgtcctcag gtatccagac atggcagaac atgcctgtta tgatgaaggg tgaagaatgg
     3121 ataacggttg taaatgcccg taatcaatat accccggggt caacggaatg gatgaaaccg
     3181 ggagaacttg ccaacctgaa agcaggaaaa gaaacaatct ggctggagga ggttacacgt
     3241 accggtgtga tacaggatta tcaaattgcg gtttccggtg caaatcctaa tgttaactat
     3301 tatttttcca gttcatatga tgtcaacaaa ggaattattg taggcgatga ttttaacaga
     3361 ttgtcttttc tggccaaact gaaaacaaat gttgcaaaat ggcttgagat cggtctggac
     3421 gcgggttttt ccagacgtga ttattcagga ttttccgcta acattggtac tgcacaggta
     3481 atgtcccctt atggtgtcat gtaccgcgac gacaagggga atcttgaaaa atatccatat
     3541 acacaatctg gtgtaaatcc cctgtggggt gtgataaatg atgttgccga taacaaggat
     3601 atcaggaata atttcaggct gaacgcgtat tcggtaataa atatcccatg ggtgaaaggt
     3661 ctgagttaca gacttaattt gcagactaac cttgataaaa atgaatcggg aaatttctat
     3721 tttgaggatt attacattgc tgaaggtgaa ggtctggaaa gatattccgc ggccgtgatc
     3781 cagggttttc tttccagggc aaacggcaat attgacaata acagtactta cagttatgtt
     3841 tttgacaata tccttaatta taaacatgtc catggagatc acagtgtgga tgtaactgct
     3901 gttgctacaa gggattttcg acaatacaaa caggtaaatt cgacaggttc tgattttgca
     3961 gccaacggga ataccacact tggcagatgg ggcttgcata aagccactgt ccagaaggtg
     4021 ctgcttaatg cagaggacag ggcaaatatt ggatattatg gccgtctgaa ctattcattt
     4081 ggcgatagat atttctttac aggctcttac aggagagatg gcgcatcggt atttggtgca
     4141 aataataaat gggcaaattt ctttgccgcg ggaacagctt ataaaatctc gaatgaaccg
     4201 ttctttaata ttgatttgat caacagtctg aagcttaaac tttcctgggg acaaaacggt
     4261 aatcagggca tcagtcctta cggaactctt tcaacggttg caaacgctgc atccggaggt
     4321 tcaagatatg aattctcaaa cacgggaagt acaatttatt acgggctgta ccagagcgct
     4381 ctcgggaatg cagacctggg ctgggaaact actgagtcgt ggaatgcagg ttttgaatca
     4441 gagatcctga acaaccggat ttcagtcgac atggatgtct atttctcaaa aacaactgat
     4501 cagatattta caagaaatat tcctgcaatg acaggtttta atacaattaa aacatccatg
     4561 ggacaggtta ataatacagg cattgaattc aggataagca gtgtgaatat taaaaaatct
     4621 gactggtcct ggaacagctc attcactttt tggaaaaaca acaataagct ggtacatctt
     4681 tatggtgatg atcttgatgg tgacgggaaa gaggatgacg atattgcaaa cagtcttttc
     4741 ataggtaaat cacttggggc aatatatggt tatgaacagg atggaattgt tcaggagaat
     4801 gatgcagaat atattgctct tacaggagct gccccgggtg caccgaaata taaggaccat
     4861 gacaaggttc ccggaattac tgcaaatgac cggatcattc tgggttacac caaggaaaat
     4921 ttccggtcaa gcctgagcaa tattgccggt tatagaaatt ttgaactata tgtgatgatc
     4981 atgggtattt tcggcggaaa taaccattat ctgaaaagta atactgctgc ataccttaca
     5041 tccggaacag gcctgttcaa tgctaatatg acatcaaaac cttactggac tcctgaaaat
     5101 aaaagtaatc tttatccgtc ggcaactttt gcaggagacg gaagatatct tgcacttcag
     5161 tcaagaggat ttgtcaggat tcaggacgtt tcattatcgt acacttttaa caagagattg
     5221 acggatgttc tgaaagtcaa ttcactaaaa gcttatctga gtgttcagaa tatggccaca
     5281 tttaccaact ggtttggagg agatccggaa acaggaacta ccgtcaggga aaatacatat
     5341 cctgttccat caacattctc agttggtata aatgcaactt tttaacatat aaaaaatgaa
     5401 agcaatgaac aagattaatt ttataaagca tatctggata ctgattctca ttttcgcgtt
     5461 attcgcatgt gagtctgata aagagttcct caatgaaaaa ccaacaacgt tttatacaac
     5521 tgataatgca ttctctacat ctgcccagat agatcaggta cttgtaggaa tttattccca
     5581 gctgagagat ctctgggcaa atcccggtga agcagcgtgg atatttgttt tccgtggtaa
     5641 cggtactgac atgttcgatg taccaagcat cagaagaggg aatacattta ataattatgg
     5701 tacaataaat ccggatcatt ctcatttcct gaatatttat agtgcatggt accagataat
     5761 ctcaaaagct aatctggtgc tttatgcttc ggaattgcca cagatcacat ggtcatcacc
     5821 tgcggaaaaa gcctatgtgc ttgcccaggc aaggttcttc agagcatttg catacaggaa
     5881 tctcggtgaa cttttcggag gagtgccgat tgtgactgag atttccaaaa cggcgaaata
     5941 tgattttgaa cgtgcatcgc ggctggaaac ataccagttt gcaattgatg aacttctcgc
     6001 catagagaat gatataccgg taacgaatgt atcaggtggc cgtattgtca gaggagcagc
     6061 ccaacacaat ctttgtgaac tctaccttgc cctgggtacc caactggcgg ctgatggaaa
     6121 ttcgtcggga gcacagacag ccttcaccaa ttcaatttct tatgctaata aagtgattga
     6181 cggtggaata tattctctga taaataaccg ctttggaaaa agaaaaaatg agttgacaat
     6241 aaatattcct gtatacagcc agggaaatac aagtacacct gttgataatc tcgaacaaac
     6301 cacgaaccat tactgggata tgttccagga aggcaatgtg aattaccagg atggtaatac
     6361 agaatgtatc tgggcacttc aaacagatta cgcggcatat aaagctgaag acggcaaatc
     6421 gaaattaccc tattcccgga cttacgggcc ggtttttcgc gatggtgcta aaaatcatct
     6481 tacgggtact cttgaagatg tgggaggaag aggtatttca caggttatcc cgacaatgta
     6541 taccagagat cttatttatg aggacaaatg ggcagaagat ttacggaatt cggatgttgt
     6601 ttttcgcaga acatttatcg gcaatgttgt cacatcaccc tattatagaa tagtagtgcc
     6661 ctgggatata atgtattatg aaagtgccga tccttctacc aacaataata accgcagtct
     6721 ctgttatcct gtttcctgta agatagctac tgataaatat acaggacttg atgccggaga
     6781 aaacagaagt aatcttttcc gtgatgatta tttcatacga ctttcggaaa ccatccttct
     6841 ccgggctgaa gcgaaacaac gcagtggtga caagaacggt gcggcagaag atataaatct
     6901 tctcagaaac cgcgctcaat gcacctatct ggttacagcc gctgatatgg atgataaatt
     6961 caatctcatt cttgatgagc gggcgagaga actggtctat gaagagtgcc gctggaatac
     7021 actactgagg atgggtggta ccatagctgt tgaccgtatt gctcaatatg cttactggcc
     7081 cgaagcccag gcaaccctga catttgatta taacctgtgg ccgattcccc agactgttat
     7141 tgacacaaac aaggacaagg tccttgaaca gaatccggga tggaaatgat aagaattttt
     7201 tccggaaggc ttgtgccatt tttaattctc agtctgattt ccggccaggc ttgtaaaacg
     7261 acagtaacaa cggatggatt caggaaagaa ataagtaacg acctgagggc ggatttcctg
     7321 aatccgcctt cttatgcacg attgagaaca ttctggtggt ggctgaacag caatgtgacc
     7381 aaagagtgta ttacacagga tctggaagcc atgaaggcta acggttacgg gggggcaatt
     7441 atatttgatg caggttcttc aaattacacg gtcgccaaaa aaacagatgc aggccctgcc
     7501 ttcctgagtg acaaatggat ggaattatac ggtcatgcca taaaagaagc tgatcgcctt
     7561 ggcctggaac tgactattaa tgtccagagc gggtggaacc cgggaggccc ttcggttaca
     7621 cccgaattcg cgatgaaaaa gataacctgg agtgaaacgg atttatccgg tccctcaaaa
     7681 atcaattccg ccctgcctga tccaacatca caattatttt acaaagatat tctggtacag
     7741 gcagtcagaa aattttctgg agatcaggat tcccctggaa tcaaaaatca tgcaattaaa
     7801 acactacttg aaaggatagg ctggtcaggg atttatcctc tccacaaatt aagagaagat
     7861 tatgatacgg aacctctcca cacattaaac aaaggtgata taatagatgt aacaccgttt
     7921 tataaggacg gtattctgac ctgggatgtg cctgatggag agtggacaat tatccgttat
     7981 gggatgacat gtaccggagt aaaagtttca acctcgagtg acggatggga tggactttcc
     8041 atggatcacc tgaacaggaa tgcattaacg cgatacaatg aagatgtgat ttccaggtta
     8101 attgccaggg caaagcagga gggaaattct cttaagttca ttcataccga cagctgggaa
     8161 atgggagttg ctaactggac acagggattc atggaaaaat tcagagaatt gagaggatat
     8221 gatatgactg aatatatgcc ggtcataaca aacaggatcg tgggtaacag agatatctca
     8281 aaccgttttc tccacgattt tcgcagaacc gtgagtgatc tgattgctga tgaattctac
     8341 aagacttttt cggaaattgc ccggcagaat gaccttttta ctcatcctga atcaggcggg
     8401 cctcattcag ctcctattga tgcaataaaa accatgggat acaatgatgt tcccatgggt
     8461 gaattctggg tgcgatcaaa cacacaccgg gtatctgatg cacaaaggct atgtgttaaa
     8521 caaagcgcca gtgtcgctca tgtttacggt aaacagttcg tggcagcgga aggaccgaca
     8581 agcataggtc cacattggga aagaccgcca agagattgca aaaatgtaat tgacaggata
     8641 ttttgttccg gagtgaacag gattgtctgg cataccttta cagcatctcc ggatgaatac
     8701 ggagaaccag gaaatgaata ttttgccgga acccatttga acagacatgt aacatggtgg
     8761 aaagaatctg aagaatttat acgatacatg aaccgatgct catttcttct ctcacaagga
     8821 aaatttgttg cagatgccct ttattactat ggtgacgata cacctaattt catttttatc
     8881 aaagaagaag ttacagatct tggccaggga tatgattggg ataaatgcag cctcgatgtt
     8941 ctacttaaca gagtcagaat taaagatggc aaaattatac ttcctgatgg aatgacatac
     9001 cggattctgg ttctgcctga ggaaaaagct cttaatatca aacttttaag aaaactcgag
     9061 aaactggtgc atgatggatt aatacttatc ggtccaaaac ctgagaaatc tactggatta
     9121 acagagtatc cggaggttga tgcagcggtt tctgaaattg ctgaaaaact ctggggaaat
     9181 attgatggca atggtataaa agtaaataag ttcggaaagg gtaaagtgat ttgggggttg
     9241 actccgggag agacactcag atctgttaat atcaaccctg attttagtta ctcatcagca
     9301 gccgacagta cctgcctgga atatattcat cgttctaccg gaaaggagga aatatatttc
     9361 gttgtaaatc gtctggcacg tcatggtatt tatgatactc aatacaggta tcttactgat
     9421 ctcccggaca gatatgaaga ggttgtatgc aaattcaggg taacagggaa aattccggaa
     9481 ttatggaatc ccatgactgg tgaaataaaa caaccggcag tataccatga tgatggatat
     9541 tatacttatg tgcctttgca tttgaatcct gaaggttcgg tatttgtgat atttgtcgct
     9601 aattctaatg ataaacatat cataaagatc gtgaaagacg gggaagagat ttttcccgcg
     9661 gcagtgagac ccggatccaa attccctacg atagaatgtt attacactga ggaaggggta
     9721 ataacaacct tttcccaacc gggaaattac acactccgct ggtccgatgg cacagaaaat
     9781 acaacaaatg taaaagaacc attatccgta atcccaatag actcttccat caatcttaaa
     9841 ttcctgaacc aatggggtcc gaaagagact atagctatta gtgaattcaa atcctggtcg
     9901 gattttgaag ataaaaggat aaaatattac agcggacctg cagaatattt aacagtattt
     9961 aatctaaaaa atggaagtct tgccggtaaa aaggtactta ttgaccttgg taatgttcag
    10021 gagattgccg ctgtatggat taatgatgaa tatgcaggag tagcctggat cgctcccttt
    10081 attctggata ttaccggctt tgtacatgag ggtaataata aactgaccgt aagagttgtg
    10141 aattcatggg taaacaggtt gattggagat ggacacctgc ctccggaaaa gcgatacacg
    10201 cgaacaaatg tccttaaatt tgaaggaggt gacaaggatc atttgttaag aaaatcggga
    10261 ttgacggatg atattaaact gattatcatt gacgaaaaaa tcattggaca tttctaaaaa
    10321 ttatttatta tgtaaataag cccgagtcag gattagccaa tccattaatt atttacagcc
    10381 ggtttattag tcatgaaact ggataaggga atttgaataa aaagcggata ctgtgtatag
    10441 tatccgcttt catgactggt cgctgccttt aattagtatt tcatggtatt atttgaagac
    10501 tattctcagc attttctgat cttccacggc cattccgtta ttttcagccg gcttccaggc
    10561 cggaccttcc ctgatcagtc ttattgcctc ggcggtatat tcttcacccg gactgctcag
    10621 tactttaatt ccgtcaacat cgccgctggt ttttacaaca aagcttacaa ccgctactgc
    10681 attttctcct tcaggcagtg tttggggttt tcttatattc tcctcaatgt atttgctgaa
    10741 actgttgtca ccgttgaccg gctgcggggc tatatagcca ccctgatcat ttccggcctg
    10801 tcctgcagat tcctcacctg cagcagtgct tccggcaatg atgtccggtg aatcaccgct
    10861 gccggccctg gcagttaata cggtctgatc ctgaatagcc gatgaatccg ttacattatc
    10921 cgtggcaagc agttcactgt tgtcaccgga aaatgaatcg cctgccaggg gcagtatttc
    10981 cggatcagga attatttcgg gaggcggagg cagtgtcctg atatccggta tggccaggga
    11041 ggccattgat tctgcttgct gaaaggcggc aatctgttga ttgtccttta ttacaggcag
    11101 aacaacggga gctgccggaa ggctgctgtg aagaaatctg aagggcttga attgctttac
    11161 ggaaagtttc ttttctgcca caagatatgc cgaagtagct atcaggagga ttattacaga
    11221 ggctataata ctgtagataa gcctcttttt acttttaatc cttttttctt cctcttcctc
    11281 ggcaaagttt gtgaagaaag gaatctcttc ttccggaaca tcggcaaaac caactatggc
    11341 tgcatcagca tactggtctt tcctgatatt cttctggaag gtgaattctt ccctttttgt
    11401 catttctcct cttacataac gctggaattc aaagaggata ctattggtaa tttcgacatc
    11461 ttgttctttc atttctcttc aattcaaagc ataaattcta ctatattccc gggcgaactt
    11521 tttgtttaat cccgtaatat gactttcttt ttctgttctg ttttaaccag accccggata
    11581 agcctgccgg agtcttgtta aatttaaata caaattgacg tagaatctaa attttaatgg
    11641 aataataggt ttcgtcgcat acgggaaaga tacgttcgtc aaaatttttc gggttctggc
    11701 aaaaaaaacc gtcattttta ttgtataaac tgcctttttc tgttgcagat ttcatggttt
    11761 ttaccatgga atcttaagca aaccaggacg tgaatgacct gacaataaca ttactgcact
    11821 ctgtgacaaa caggtatgtt gaaccttata gtttcagcat tgacagtctg atgagtcttt
    11881 tcccgaaaaa ctcctgtttg gagcagatga ttttctgtcc gtttatttca accgctccgt
    11941 atcttttaat tcttttctgc aaagccctgt gtggtacagc tgttatcctc gactgccgtt
    12001 tcaccggtta cggattttag gcctgcatga tcatcctgta ttctgtggtg attatcagtg
    12061 tatgaatata ttttttcact ctgatgtatt tccttttggc tgattggtta tatttacggc
    12121 atcaagctct tcgcggactg ttttaaaata ttgtcatgga gcatcccgac aacctgatcc
    12181 tggtgatttt cggagcatca ggcgatctta cctcccggaa actcattcct gctcttttct
    12241 cactgaagag ccagaagctt cttccatcgc aatttgcaat agttgggagc gggaggacct
    12301 cttttagcgg ggatgaattc agggaaaaaa tgaggacagc catttttgcc gttcacgggg
    12361 gcaggaatcc tgattcccgg ctggtccggg aatttttaga acagatttat tatgtgacaa
    12421 tggacagtgc tgagccggaa aactattccc ggctgaaagg aaaactcagc ctgctgaatt
    12481 ctgaatgtgg tacctgcggc aattatattt tctatctggc aaccccgccc gtattgtatg
    12541 aagtgatttc cacaaatctg acaaaagcag gcctggcaga ccagtcggaa ggtttcagac
    12601 gcttcataat agagaagcct ttcggatatg atctggcttc tgcacaaaaa cttactggga
    12661 aacttcacga actgataagt gaggaccaga tatacaggat agatcactat ctgggcaagg
    12721 aaactgttca gaacatactt gtgacccgtt ttgccaacgg aatttttgag ccattatgga
    12781 acaggaatta tgtacacagg attgagatta catcttccga aaatttaggt gtcggagaga
    12841 gagggggtta ttatgataca tccggcgccc tgagggatat ggttcagaat catttgctcc
    12901 agatggtcgg acttaccgca atggagcccc cttcatccat gatgccggat gcgataagga
    12961 atgaaatact gaaggttttt cagtcatttc agccgttgcg ggaggaggac gtgccgcatc
    13021 aggttatacg ggggcaatat ctggagtcca ggatttccgg tgaacggatc cctgcataca
    13081 ggtcggaaaa gggcgtatcc ccggattcca ggacagaaac ctatgtggcg ctgaaattct
    13141 ttgtgaacaa ctggcgatgg ggaggtgttc ccttttatat caggacaggt aagcgtctgc
    13201 ctgtgaaagt gactgaaata gtgattcatt tcaaacgtac cccccaccat ctttttcaga
    13261 gaaatgaagg tccgatgaat tccaataagc ttattctgag aattcagccg gatgagggaa
    13321 tactgctgaa tttcggcatg aaggaaccgg gatcgggctt caatgtaaag aatgtggata
    13381 tggatttccg ttacagcagc ctggctgatg taagagttcc ggcagcatac gaaagacttc
    13441 tttttgatgt tatggtaggt gattccacat tattttcacg ggatgatgtt gttgaaactg
    13501 cctggcggtt tcttgaacct gtgcagaatg catggaaaaa tgatcccggc attaaactgt
    13561 acggataccc tgccggaaca tgggggcccg atgtggcgga caatcttgtt gagggctgcg
    13621 gactgacatg gagatatccg tgcaacaatc tggccgagga cggaaaattc tgtgaattgt
    13681 aattttaaag aacaagctgt taaaaccggg attccgaaca ggacaggaat ggaaaaaaca
    13741 gtaaggatat atgattcccc ggaggagctt gctgaggcat ttgcgcggga gctggccagc
    13801 agtttcagga atgcaaccga aaagggcagt gtttttaccg tggcgctttc aggcggaagc
    13861 actccttctg ccttgtattc ccttttggct gagaaatacg ctgattctgt aaactggaaa
    13921 tatattcact tcttctgggg tgatgaaagg tgtgtgcctc cggatgatcc ggaaagcaat
    13981 tatggaatgg catttgataa attcctcggg aggatcgata ttcctccggg aaatattcac
    14041 agaatagcag gggaagggat accttccgtg gaagcactca ggtattccgg tgaaatttta
    14101 ctgaatacac ggagttttaa cgggttgccc gtttttaacc attttattct gggaatgggg
    14161 gaggacggac acattgtatc catttttcct tcaaaccgtg aaatgttcca cagcgataaa
    14221 atatgtgatg tggcagttca tccggtgacg ggacaaaaaa ggattacggt tacaggaaaa
    14281 gttatcaaca attccgaatc agttactttt cttgttacgg gaaagtcaaa ggcagaaact
    14341 gttgaaagga ttttcatgaa ggacaatggt tttccggctt ctgatgttat gccctaccat
    14401 ggcaggcttg cctggttgct tgataaagag gctgcttcaa agatcagcgg gaacggtcag
    14461 tgttaaaaaa cttacagaat ttcttaaagt agatttatta ttttaaacat gttgttgaat
    14521 ctaatactta tgcatcatgg tacaggagaa agcaaaacag caggtaaagg tcaagaagaa
    14581 agcggccaag accctgaagg aaaaacgtgc agaaaaaaag actaaaaaag ccgaaaagaa
    14641 caaataagaa atgcccgggc atgcggataa aaaaagaggc tgcaggcagc ctctcttttt
    14701 atttccagtc gttttattga atctttttaa catttaccgc attaatccct tttttgcctt
    14761 ctgccagttc aaaggtaaca tcatcattct cctttatttt atcaacaagc ccagatgcgt
    14821 gcacaaaata ttcattgtcc gaatcagcat ccttaatgaa tccaaatcct tttgagacat
    14881 taaaaaattt aacctttcct ttgttcattg taattgtgtt ttaaaattaa gcgcgctaaa
    14941 ttacaaattt ttattttata tatatccttt cttattcatg ttattttaat aacattgcaa
    15001 aaaatttgtt ttatgcatca ttaatctgtt cttttaaaac taagttgttt ataatgagaa
    15061 agtggcgaat tgaagattct gcagaacttt acaatatcaa cgggtgggga gtcggctatt
    15121 tctccattaa tgaaaagggg aatgtcattg taactccaca ggaaaataat ccgggggtcg
    15181 accttaagga actgatagac gggctcagcc tgagagatgt tacaactcct gttctgcttc
    15241 ggttcccgga cattcttgat gatcgtattg taaacattac gaattgtttc agaaaggctt
    15301 cggaggagta tgactacaaa gcccagaatt tcataatata tcccattaaa gtcaaccaga
    15361 tgcggccggt cgttgaggag atagtcagcc atggcaaaaa attcaacatc ggactagaag
    15421 ccggatccaa gcctgaactt cacgctgtaa tagcaataaa taccgaggct gaatcgctta
    15481 ttatatgcaa cggatataag gatgaagatt atattgaact tgcactcctg gcccagaaaa
    15541 tgggaaagat gatctttatt gtggttgaaa aactcagcga actgaaactt atcaccagta
    15601 ttgccagaag actcaaggtt aagccaaata tcggcatcag gatcaagctc gcatccaccg
    15661 gaagcggcaa atgggaagaa tccggcggtg atgtcagtaa attcggcctt acatcaagtg
    15721 aattgcttga ggcaatcgat ttcattgaaa agaacaggtt gaaaagcagt ctgcggctga
    15781 tccattttca tattggcagc caggtaacca agatcagaag gattaagacc gcactgaggg
    15841 aggcttcaca gttctacgtt cagttgagaa aactcggttt caatgtggaa tttgtggata
    15901 tcggcggcgg gctcggtgtt gactatgacg gaaccggttc cgccaacagt gcaagcagta
    15961 ttaactacac ccttcaggaa tatgtaaatg attcggtcag tacatttgtt gatgcaagca
    16021 acaagaacaa tattccgcat ccgaacgtaa taaccgaatc aggccggtct ctcacagccc
    16081 accattcggt gcttgtcttt gaagtactgg aaacagccac attgcctgaa tgggatgttg
    16141 aagaagaaat aggtgaaaat gatcatgaac tggtaaagga gatctatacc ctttgggata
    16201 atttcaatca gcagcgaatg attgaatcct ggcatgatgc gcagcagatc cgtgaagaag
    16261 cacttgaccg tttcagtctt ggcattattg atctgaggac aagagcccag attgaaaaac
    16321 ttttctggtc catagccaga aaggtgtacc agactgccgg gaaatccaga catgttccgg
    16381 aggaaataag gcaaatatca aaaatactgt ctgacaaata tttttgcaat ttctcgcttt
    16441 tccaatcact tcctgatgcc tgggcaattg accagatttt tccggtaatg cctattcagc
    16501 gcctgaatga acaacctgtt gttgctgcca ccatacagga catgacctgt gattcagacg
    16561 gaaagattga taatttcata gcaaccagca ataattcaca ttttctgccg gttcatcacc
    16621 tgaaaccaaa agagccttat tacctgggtg tgtttcttgt gggagcatac caggaaatac
    16681 ttggtgatct gcataacctt ttcggtgata ccaatgcggt gcatgtttcc gtggataagg
    16741 acggatatag tattgatcag gttatagatg gcgaaacaat agcggaagtt cttgattatg
    16801 tgcagtataa tccaaagaaa atggtaagaa ccgtagagtc ctgggtcact tcatccgtga
    16861 aggcgggaat aataaccctg gaggaaggcc gggagttcct gtcgaattac agatcaggcc
    16921 tgtatggcta tacttatctt gaatgaagat aaccgcagat taattgatta tttgatagaa
    16981 ttctcatcat ttttttttaa acttgtaaat actaataata aaccatttaa tcttacagga
    17041 catgagtaat tcttcatttg atttcaatgc actgatcagg gattccaaag agatccttaa
    17101 agatccgaag gcatatttct catccatgaa aacaacggga ggaattgctg aaccggttat
    17161 caaagctgtt gtttacggtt tggttgccgg aatatttgca tttatctgga gcctgctgaa
    17221 aatcggacct gcctcttatg gcatatcggg aggagccgtg ggattcatgg cgcttgtatg
    17281 gtatgttgtc ggtgctgtta tcggcctttt cataggtgca gttattctgc tcgttatttc
    17341 cgcaatatgc aagggaagta cggattttga atcaaatgta agagtcactg cagccctgat
    17401 ggttatgatg cccatatcag ccttcctcgg cttttttatg ggaataggca tcaccctcgg
    17461 taccatagta agccttattg tttcgctgta cggactttat cttctttacc atggacttat
    17521 tgaaaccctg aaagccaatg ccggaacttc cagggtactt gtccttgtac tcgcagccct
    17581 gcttgttata ttctcacttg ttggaatcgg agcaaggaaa aaggcggcca gttttatggg
    17641 ggacttcaat atggatcaga tcagggaatt ttacgaggat gccggcaata attagaaaaa
    17701 cccggaaata caaaatatcc cggacccggt gaatttcagt caccgggttt ttcaattgtt
    17761 tttatacttt tacaaatccg tatcggacag cgttggctta tccggaaata attcaggcat
    17821 gaagaactgg ttcttttcaa agaatatact tgttgtttta atctacagaa tcctgataat
    17881 tcttttcctc ttttccttaa gcagaatcaa attttatatt ttcaatcata aaatgtttc
//
LOCUS       DAOSPX010000026        17443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3385_length_17443_cov_4.847078, whole genome shotgun sequence.
ACCESSION   DAOSPX010000026 DAOSPX010000000
VERSION     DAOSPX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3385_length_17443_cov_4.847078"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PLZ75_02495"
     CDS             <1..903
                     /locus_tag="PLZ75_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HOS71299.1"
                     /translation="GFFDIILADVPCSGEGMFRNLSVRTEWSEASAELCALRQKRILM
                     DVWPALREGGILIYSTCTFNPAENEDNIAWLAVKAGIESLRPDFSEYEGITEIERGKI
                     YGYGFYPGKISGEGFFVSVIRKTEAVPGKHGRTVRRQDSAGVSGEERAAVERMADLPS
                     ERLVKHRDEVIALPCSKDDYLRIADEVKIVKAGTRILKSGGVPLHDLALSVKCRTDSF
                     PAVNLAYGQALAFLGRSVIAPENIPRGWNLVRYRGINLGFINNVGSRINNYYPVEWRI
                     RQQIPGEPGADIIKWTGYNQPGRI"
     gene            1004..2149
                     /locus_tag="PLZ75_02500"
     CDS             1004..2149
                     /locus_tag="PLZ75_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOS71300.1"
                     /translation="MNKLSREFQIFVKPVGAACNMRCSYCYYLSRADLYPGNKFPLMS
                     DEMLERYIVQHIEATTDDVVLFSWHGGEPVLAGLDFYRKAIAFQKKHNREKKRIINGI
                     QTNGTLLDDELCRFMRDEKFIAGISLDGPQEYHDRYRTDSRGKGTFKKAMRGYELLRK
                     YGVTTEILCVVNSFNVRHPLALYEFFRGTDARYITFLPLVERDVHSENEVTADTVPAE
                     QFGIFLSEIFDRWSADDIGRIEIQIFEEATRRAFGREHSLCIFRVDCGGVPVVEHNGD
                     FYSCDHYVDREHFLGNIAEGTLAGFLDSERQQAFGRAKSMTLPRYCLECEFLDMCNGE
                     CPRNRFISTPDGEAGLNYLCRGYRIFFAHCQPFISALRELWLMQGKA"
     gene            complement(2209..3069)
                     /locus_tag="PLZ75_02505"
     CDS             complement(2209..3069)
                     /locus_tag="PLZ75_02505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOS71301.1"
                     /translation="MEERISSLYNDYRKGLSGRREFLRKLTMITGSTAAAMALLPILE
                     DNRAIAADAKKQKELKITADFIKYPADSGEMRAYLAKPAKGKLFPAVIVIHENRGLVP
                     HIQDVTRRMAREGFLALAPDALTPLGGTPEDESNTPELIRKLDNEETVKNFVAAVRFL
                     KTHPNSNGKVGCTGFCWGGAMTNQVAVNSPELDAAIPWYGRQPSAEDAARIKAPVMAH
                     YAENDPGINAGIADFEEALKKNYIEHYFFTYPGTGHAFNNDTNPSRYNAGAAQLAWAR
                     TVGFLKEKLK"
     gene            3201..4871
                     /locus_tag="PLZ75_02510"
     CDS             3201..4871
                     /locus_tag="PLZ75_02510"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOS71302.1"
                     /translation="MKRDIEIAQETRKKPIVEIAQMLGIPEEDLELFGRYKAKIPLRF
                     IDEEKMKKSNLILVTAITPTPAGEGKTTTSIGLAQAMNKLGKLTTVVIREPSLGPVFG
                     MKGGAAGGGYSQVVPMEDINLHFTGDLSAVEKAHNLLAAMIDNNIQLKEGNLGLDPRT
                     IEWKRVMDMNERALRDIVIGLGGTTEGVPRQSGFNITAASEVMATLCMASDLTDLKER
                     LGRIFVGYTHAGKPVFAADLKAQGAMAALLKYTLMPNLVQTLEGTPAIIHGGPFGNIA
                     QGTNSIIGTKMGLSLSDYVVTEAGFASELGAEKFFDIKCRFGNLRTKTVVIVATIRAL
                     KYHGNEKLAALQNENLTALKKGFDNLDKHIENMRSFGFNPIIAINKFATDTDAEVNLL
                     FEHCREQNVKVAINDGWARGGDGAIELAQAVIESVAGGTPEFKPLYDLDWSFEKKIET
                     ICRKMYGADGVEYTPKAKKQLERIDNLGISDLAVCIAKTQKSLSDEPGLRGRPQGFTV
                     KIREVELSSGAGFIVPIAGAIMRMPGLPNVPAAEKIDIDGEGRITGLF"
     gene            5115..>6744
                     /locus_tag="PLZ75_02515"
     CDS             5115..>6744
                     /locus_tag="PLZ75_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS71303.1"
                     /translation="MNRLVLFFIMALLVISGCRRYSKYEGVEFTEKEPRDWENPGMIG
                     QNKEAPHVSLISYGDRESALAADKKAAPNLLSLDGIWKFNLVKAPGDRPYWFFKDDYD
                     TRDWDDIEVPSNWEMKGYDVPIYVNITFPHKNDPPFIQHDYNPVGSYKRNFRIPSQWR
                     DKEVYLHFGAVASAFYVWINEQLVGYSQDSKTPAEFNITRYLKRGRNSIAVEVYRWCD
                     GSYLEDQDFWRLSGIQRSVFLHARPRTHIRDFYAVADLENDYRDGALKLDVELNTADA
                     TDAGDAGSEYIVEASLFDGSQELYSESKEVRMTGNTAGVSFLKNFPGIKRWSAESPDL
                     YSLVITLKNRNGRALESVSSRIGFRKVEIRDSQFLVNGSAIYLKGVNLHEHSDVDGHV
                     ISEELILKDIGLMKSNNINAVRTSHYPQQELFYELCDKYGLYVIDEANIESHGIGYDK
                     DVTIADKPEWAPAHMARMQAMVERDKNHPSIVIWSLGNESGDGHNFLDLYKWTKDRDK
                     TRPVQYERAEKSTNTTERHTDIVCPMYDRVWEVEA"
     assembly_gap    6745..6754
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6755..8368
                     /locus_tag="PLZ75_02520"
     CDS             <6755..8368
                     /locus_tag="PLZ75_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS71304.1"
                     /translation="AKDPASDRPYILCEYAHSMGNATGNLQDYWDVIEKYPILQGGFI
                     WDWVDQGILQTDENGEKYWAYGGDFGEEGVPSDGNFCINGLVLPDRSVKPGLNEVKKV
                     YQYVGFESVDLKKGLIRIKNKYDFTSLSKFDLDWEIMADGSVIQSGRAPLSDLQPGKT
                     ADIAIPLQMPEPLPGTEYFLNLRISGSEAWGIIPEDHVYATEQFKLPAEGAPVPLKQS
                     DLAVLQITTKEFLLEIGGSDMLLTFNLETGRLESYKYRGRELLLKGPEPDFWRPPTDS
                     DYGYNMDRKLGVWKKAGERSILKSVDIRQPELGRVLVVFRYDIPDTEGKKIAGYSSAF
                     TVLGSGDVIISNSFAKVSDQIPEIPRMGMQMQLPVELTNLKWFGRGPHENYSDRKTSA
                     ETGLYESTVADQYVPYIRPQENGYKTDVRWLSLTDNDGNGILVSGNPLFCFAALNNIH
                     DDFESPGKLSQYRKDAKSANTHTNDVKPRDFVNLNVDLAQMGLGGDNAWGAEIYRQYR
                     LLGSKYEYSFRIRPVVRGDDVIKLAKGRY"
     gene            complement(8393..9454)
                     /locus_tag="PLZ75_02525"
     CDS             complement(8393..9454)
                     /locus_tag="PLZ75_02525"
                     /inference="COORDINATES: protein motif:HMM:NF027708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HOS71305.1"
                     /translation="MKRLSVFLLLIISTTLLLTGQRKIINLPDLPGYVTLKCDFHLHT
                     AFSDGNVWPTIRVNEAYRDGLDAIAITDHLEYTPHKDYIPVDHNAAWKITRNTARDYN
                     LILVHGAEITRSMPPGHVNALFISDANALAVDSVWDAFEATIKQGGFLLWNHPGWKAQ
                     QPDGIPRMYNIHHRLIRNKWLHGIEFFNHKEYYPLVMTFALENNLAVFANSDMHGAIS
                     EEYAGKTHRPMTLVFARERSHDSLKEAMFAGRTLAYFDDMLAGRKEYALPFFHQCISV
                     GKPYYQNEKSIYLEITNRSDIPFYLTDGVPGTPSSITLPANSATRVVLSKSNTNPLIY
                     NVRNIITGESEVLKVELKY"
     gene            complement(9588..10229)
                     /locus_tag="PLZ75_02530"
     CDS             complement(9588..10229)
                     /locus_tag="PLZ75_02530"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HOS71306.1"
                     /translation="MGLQVEDLEDPSRYRELLKLPYEELIDFVVDYIRRKSEVMVFFW
                     AVCIIFLGFAITIRINIAGYFPLMSIALHTFLGLIVFPLLLIPFHELVHIIVYLISGA
                     RRIRVGMDLRQYMFYVTAHRYVTGQKKFRLVALAPFVFISLALILLVFILPGLWKWSM
                     SLLLFVHATMCAGDFAMLNFYFFNRKKKIYTWDDFDRKIAYFYEEIQGTDTEE"
     gene            complement(10645..11412)
                     /locus_tag="PLZ75_02535"
     CDS             complement(10645..11412)
                     /locus_tag="PLZ75_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS71307.1"
                     /translation="MKKLPLFIVFILSVQLCFGQKINKAEYFIDSDPGFGKAVTIPVS
                     TPAKDVSLNFLANLSGLSQGFHMIGVRARDDNGAWSMKHEQLFYLFRRVPAASPKITG
                     LEYFIDNDPGFGKGTKVAVRVPADRVTLEFIADLTGLSSGSHILYMRARDEANRWSTI
                     HTHAFTLNVTAIGEEIVPWFKMYPNPSEGNFVIDFDDLQRGSVKVTITDLNGRIVYSN
                     ELNGNIIPLSVDLPNGIYTLRAESGSQHFIQKLIIRR"
     gene            complement(11432..12424)
                     /locus_tag="PLZ75_02540"
     CDS             complement(11432..12424)
                     /locus_tag="PLZ75_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71308.1"
                     /translation="MKKLLLLSTLFSLFCGIIFGQHLIRVNNDPAANADYTTIQSAID
                     GAQPGDTIYVEGSPVNYAGAKIDKKLVIIGPGYFLADNDSTQANIHVAEISASLDFIK
                     GSEGSKVCGLIIDQTIYIRDDEIVVSRCKINKLQIYSNSNNITITQNFISDISTYYVN
                     LTNSVISNNITNYISVSKASYPLQITNNVFIGGTQTYVLSGVYNSTISNNIFIHPTRK
                     MQENTGNTIINNIFSYDGTDANGNKYNIDMTDVFVDPDGTLGYSQDARYKLKAGSPAI
                     GAGVTGVDCGVFGGATPYVLSGLPPIPRIYEAAIAGTAYSDKGLSCIIKIKAGK"
     gene            12661..13320
                     /locus_tag="PLZ75_02545"
     CDS             12661..13320
                     /locus_tag="PLZ75_02545"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOS71309.1"
                     /translation="MATDVRHFFLLIVFIILPVPLSGQTDTSRKVNERTVIPDSAREE
                     IHVLQKVERNGLVLPEVEIKEVTIIGRRQESYNPRKIRAQERQYDRLVYNLKKTYPYA
                     VITREKLSEVNTRLEQISDEKERKKFLKDFEKELFAEFEADVRNMTITQGRLLIKLID
                     RETQNTSYELIRQYRGFLSAVFWQGIARIFGSNLKAEYDPFGEDALIELIVRDIEMGW
                     L"
     gene            13549..14109
                     /gene="thpR"
                     /locus_tag="PLZ75_02550"
     CDS             13549..14109
                     /gene="thpR"
                     /locus_tag="PLZ75_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOS71310.1"
                     /translation="MMKRIFTAIKIIPEKSFLDIHSSFQAILANEKIKWVDTSNIHLT
                     LAFLGDIDEKRIPELAVMLKLKCAGSGEFDFTLAGAGIFKNLRDSRVIWLGVKEYEKL
                     ISLYEEIKAGLKETGFQTEDRQFRPHITIGRIKFIRDAGNLGKALEMYRDQFIQKVNV
                     EELVLYESILKPSGPVYKALEKFRLV"
     gene            complement(14171..15172)
                     /locus_tag="PLZ75_02555"
     CDS             complement(14171..15172)
                     /locus_tag="PLZ75_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOS71311.1"
                     /translation="MKDANKSVTACVVSISMFFIVLGISCKKEKSVLPEVDTTEVSVV
                     TPTSASCTGVVISAGSTKLIEKGLCWSDSPSPTIEDSTVSTHLYPYLVPFELRIGGLK
                     PEITYYVRAYATSEAGTSYGNELLFATPADLTGEKGTVADSDGNIYQTIGIGSQIWIA
                     ENLKTTRYNDGTSIPLITGNREWSQLFKPAYCWYNNDVINKDIYGALYNWYTVQTGKL
                     CPSGWHVPDLKEWKIIETFLGGPDVAGGKMKAAGTDYWKSPNVGATNSSGFTGLPGGF
                     RADDGSFVYIRESNLLWSSTGNKSTSAWYRAQGYIASDLPIGSNTVLWGGSVRCIKD"
     gene            15637..16362
                     /locus_tag="PLZ75_02560"
     CDS             15637..16362
                     /locus_tag="PLZ75_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOS71312.1"
                     /translation="MKTSEYISLTIDRLPKGYVFTYADFITEVNKKEAVIKALNRMVA
                     SGKIMKLAKGKYYKPETTPFGKLLPDQKQVVKDLLEENGKIIGYLTGYSIYNQLGLTT
                     QVSNTIQIGRNQIRPSFKRERYTISFIKQKNTITKENIPLLQILDALRYIKKIPDAKI
                     ESSCRRLLNILKELTENDKSSLVRLALKYPPVTRALLGAMLEQLQQTEITQPLLKSLN
                     PITKYKLSGISKVLITAEKWNLL"
     gene            16359..17303
                     /locus_tag="PLZ75_02565"
     CDS             16359..17303
                     /locus_tag="PLZ75_02565"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOS71313.1"
                     /translation="MKLHHDIKLFSDTLRAASQHLDIKLEFVEKDYWITLVLSRLAKN
                     KYVDESVFKGGTSLSKGYNLIKRFSEDVDIAIINDKGKTGNEIKTIIRTIEKEITSDL
                     TEVQMDGVTSKGSRIRKSVFEYVTTDKSNKNNKLIVEINSFANPFPFQRLSLKSFVFD
                     FLQQTGNEKYIEQYDLQPFEVNVLSKEQTLLEKTISLIRFSFEQKAVETISKKIRHFY
                     DLYFLMKNPECAEFVSSDSFKKQFETILQHDREMFEEPIGWQTKLISESPLVADFPAI
                     WEQLKEKYQTELSALAYRTIPAENEVAKWFKEFIKRIE"
BASE COUNT         5104 a         3587 c         4082 g         4660 t
ORIGIN      
        1 ggcttctttg acattatcct tgcagatgtt ccctgttccg gtgagggaat gttcaggaat
       61 ctcagtgtta ggaccgaatg gtcggaagcc agtgcagaac tgtgtgctct gagacagaaa
      121 aggatactga tggatgtttg gccggccctg agggaaggcg ggatattgat ctacagtaca
      181 tgtaccttta atcctgccga gaatgaagat aatatagcct ggcttgccgt aaaggccgga
      241 attgaatcac tgagaccgga tttttcggaa tatgaaggaa ttacagagat tgagcgcggg
      301 aaaatttacg gctacggttt ttaccccgga aagatcagcg gcgaaggatt ttttgtttca
      361 gtgataagga aaacggaagc tgttccggga aaacatggca ggactgtcag gaggcaggat
      421 tctgcaggag tgagcgggga ggagagggct gctgttgaaa ggatggcgga tcttccttcg
      481 gaacggcttg tgaagcacag ggatgaagtg atagcattgc cgtgcagcaa ggatgattac
      541 ctcagaatag cggatgaagt gaagatagtt aaagccggga ccaggattct gaaatcggga
      601 ggggttccgc ttcatgacct tgctttgtca gtgaagtgca gaacggattc cttccctgct
      661 gtaaaccttg catacggtca ggcacttgcc tttctgggca gatcagtgat tgcaccggaa
      721 aatattccgc ggggctggaa cctggtccgg tacaggggaa ttaaccttgg attcatcaat
      781 aatgtcggca gcaggataaa caattattac cctgtggaat ggaggatcag gcagcaaatc
      841 ccgggcgaac ccggagcgga tataatcaaa tggaccggat ataatcagcc gggccggata
      901 taatcaaccg gaccgttaaa gagagccccg gcgggaaagg cggaaccccg gttattgcgg
      961 tggggcagag aaatttaata aaaacagaag agattcagga acaatgaata agctgtcgcg
     1021 cgaatttcag atctttgtca agccggttgg tgccgcatgt aacatgcgct gctcttattg
     1081 ctattatctt tcccgggcgg atctctatcc cgggaacaag tttcccctga tgagcgatga
     1141 aatgctggaa agatatatag ttcagcatat cgaagcaacg acggatgatg ttgtcttgtt
     1201 ctcctggcat ggaggagagc ctgttcttgc cggacttgat ttttacagga aggctattgc
     1261 attccagaag aagcacaaca gggagaagaa aaggattata aacggcatac agaccaatgg
     1321 tactcttctt gatgatgagc tgtgcaggtt tatgcgggat gagaaattca ttgccgggat
     1381 aagccttgac ggcccgcagg agtatcatga ccgctacagg acagacagca ggggaaaggg
     1441 cacattcaaa aaggcaatgc ggggctatga actgctgagg aaatacgggg tgacaaccga
     1501 aatactctgt gtcgtgaatt ccttcaatgt caggcatccg ctggctctgt atgaattctt
     1561 ccgcggtact gatgccaggt atattacatt ccttcccctg gtggaaaggg atgttcattc
     1621 ggaaaacgaa gtcaccgcgg ataccgttcc ggctgagcag tttgggattt ttctttcgga
     1681 gatatttgac cgctggtcag ctgatgatat cggcaggatt gagattcaga tctttgaaga
     1741 agccacccgc agggctttcg gacgggaaca cagtctatgc attttcaggg tggattgcgg
     1801 aggagtgccg gttgttgaac ataacggaga tttttattcc tgtgatcatt atgttgacag
     1861 ggagcacttc ctgggtaata ttgctgaggg tacacttgcc ggatttctgg acagtgagag
     1921 acagcaggcc tttggaaggg caaaatcaat gactcttccc cgatattgcc tggaatgtga
     1981 atttcttgac atgtgtaacg gggaatgtcc cagaaaccgc tttatttcta ctcccgacgg
     2041 ggaagccggt ctgaattatc tgtgcagggg atatagaata tttttcgcac actgccagcc
     2101 ttttatttca gccctaaggg aactatggct gatgcaggga aaggcctgac acaacggaac
     2161 tggttctgta tctgtgccct gcttagggtt ccaggacaag atcatgtgct attttagttt
     2221 ttccttcagg aatcccactg tcctggccca tgccagctgt gccgctccgg cattgtagcg
     2281 cgacggattg gtgtcattgt taaatgcatg gccggttccg gggtatgtga agaaataatg
     2341 ttctatataa ttctttttca atgcttcctc aaaatctgct atgcctgcat ttattccggg
     2401 atcattttcc gcatagtgtg ccattacggg ggctttgatc cttgctgcgt cctccgctga
     2461 aggctgcctg ccataccagg gaatggcagc atcaagctca ggcgaattta ctgcaacctg
     2521 gttggtcatt gctccgcccc agcaaaatcc ggtgcatccg accttgccgt tggagttggg
     2581 atgggttttc aggaatctga ccgctgccac gaaattttta acagtttcct cgttgtcgag
     2641 tttcctgata agttccggcg tattgctttc atcttcaggc gtgcctccga gtggagtaag
     2701 ggcatcggga gccagggcaa gaaatccctc ccttgccatt cttcttgtca catcctgaat
     2761 atgcggaacc agtcccctgt tttcatgaat tacgataaca gccggaaata attttccttt
     2821 cgccggtttt gccagataag cccgcatttc cccggagtct gccggatatt ttataaaatc
     2881 tgctgttatc ttcagctctt tttgcttttt tgcgtcagct gcaattgccc ggttatcttc
     2941 cagtatggga agaagtgcca tggctgctgc agtgcttccc gtaatcattg tcagcttcct
     3001 cagaaactcc cggcgccctg aaaggccctt ccggtaatca ttgtaaagcg aggatattct
     3061 ttcttccatg atctttacga ttaatgtgat atatttgact cggatataaa atttcaattt
     3121 acagaaaaaa tcataaaatc acttattttt gaagactttg tttcaagtat aatttcagaa
     3181 aaaccattaa aatcttaact atgaagcgtg atatcgaaat tgcccaggaa accaggaaaa
     3241 agccaatagt tgaaatagcg cagatgttag ggattccgga agaggatctt gaattatttg
     3301 gaaggtacaa ggcgaaaata ccactgaggt ttatcgacga ggaaaaaatg aagaagtcaa
     3361 acctcatcct tgttacggca ataactccca caccggcagg cgaaggaaaa acaacaacat
     3421 caataggtct tgcccaggca atgaacaagc tcggaaagct taccacagtc gttatccgcg
     3481 aaccatcact cggtcctgtt ttcggcatga aggggggagc tgccggcggc ggctattccc
     3541 aggttgtacc catggaagat atcaatcttc attttaccgg tgatctgtct gctgttgaga
     3601 aggctcataa ccttctggca gccatgatag acaacaatat ccagctgaag gaaggcaatc
     3661 tgggtctcga tcccagaaca atcgaatgga agagggttat ggatatgaat gaacgcgcac
     3721 tcagggatat tgtcattggt ctgggaggca caaccgaggg agtgccccgg cagtcaggat
     3781 tcaacattac cgccgcatcg gaagtaatgg caacactctg tatggccagt gacctcactg
     3841 atctgaagga aagactggga aggatattcg tcggctatac ccatgcaggc aagcctgtgt
     3901 ttgctgccga cctgaaagcc cagggtgcaa tggccgccct gctcaagtat acactaatgc
     3961 cgaaccttgt tcagactctt gaagggacgc cggcaatcat tcacggagga cctttcggaa
     4021 atatagccca gggaaccaat tcaataatcg gaacaaagat gggcctgtca ctcagcgatt
     4081 atgttgttac ggaagcagga tttgccagcg agctgggggc agagaaattc tttgacataa
     4141 aatgccgctt cggcaatctc aggaccaaaa cggttgtaat agtggccacc ataagagccc
     4201 tgaaatacca tggaaacgaa aagctggcag ctcttcagaa tgagaatctc acagcactga
     4261 aaaaaggatt tgacaatctc gacaagcaca ttgagaatat gagatctttc ggattcaatc
     4321 cgattattgc gataaacaag tttgccacag ataccgatgc agaagtgaat ctcctttttg
     4381 aacattgccg cgaacagaat gtgaaggttg ccattaatga cggatgggcc aggggaggag
     4441 atggcgccat tgaactggca caggcagtga tcgaatccgt tgccggtgga acaccggaat
     4501 tcaaaccctt atatgatctt gactggtcat tcgagaagaa aattgaaacc atatgcagga
     4561 aaatgtacgg agctgatggt gtggagtata cgcccaaggc gaaaaagcag cttgaacgga
     4621 tcgataatct cgggatcagt gatctggctg tctgcatagc caaaacccag aaatcactct
     4681 ccgatgaacc cggtctgagg ggaaggcctc agggattcac ggtaaagata agggaagttg
     4741 aactctcatc aggagcagga tttatagtac ccattgccgg agccattatg agaatgcccg
     4801 gtctgcccaa tgtgccggcg gcagaaaaga ttgatatcga cggggaagga agaataacgg
     4861 gtcttttctg aaaaacagaa atatttctta acttgtatgt cctgatcgta ttgcagatcc
     4921 ggcaggataa aaacggctgt cacatccggg ctgccggccg gccagggcgg actggaaatg
     4981 catggggata atggtcggga gatctgccat gttcctgaca gggtcagggg ctatggacca
     5041 acataacctg ctatttacag ttcttcagtt ttcccggcat aaaacatgac aattgacata
     5101 ttaattataa acccatgaac agacttgtat tgttttttat tatggcttta ctggtaattt
     5161 ccggctgtag gagatacagt aaatacgaag gcgtggagtt tacggagaag gaaccccgcg
     5221 actgggaaaa ccccggaatg atcggacaga acaaggaagc cccgcatgtg tctctgatca
     5281 gttacggcga cagggaatct gccctggcgg cagacaaaaa agctgcgccg aaccttcttt
     5341 ccctcgacgg aatatggaag ttcaatcttg taaaagctcc cggcgacagg ccatactggt
     5401 ttttcaagga tgattacgac acccgtgact gggatgatat tgaagtgccg tcaaactggg
     5461 aaatgaaggg ctacgatgtg cccatctatg taaatattac atttccgcat aagaatgacc
     5521 ctccctttat ccagcacgac tataacccgg taggatccta caagaggaat ttcagaatac
     5581 cctcacaatg gagggacaag gaggtttatc ttcatttcgg agcagtggca tctgcctttt
     5641 atgtctggat caatgagcag cttgtcggtt acagccagga cagcaagact cccgcggaat
     5701 tcaatattac caggtattta aaaagaggca ggaactccat tgcggttgag gtataccgct
     5761 ggtgtgacgg cagttacctc gaggatcagg atttctggag actgagcggc attcagagga
     5821 gtgttttcct tcatgcaaga ccacgtaccc atattaggga cttttacgct gttgctgatc
     5881 ttgaaaatga ttacagggat ggtgccctga agcttgatgt agagctgaac accgctgatg
     5941 ccactgacgc cggtgatgcc ggcagtgaat atattgttga ggcttcgctt tttgacggat
     6001 cacaggaact gtacagtgaa tcaaaggaag tcagaatgac agggaacaca gccggagtaa
     6061 gttttctcaa aaattttccc ggaataaaaa gatggtcggc agagagtcct gatctttatt
     6121 ccctggtgat taccctcaaa aaccgtaatg gcagggcact tgaaagcgta agttcaagga
     6181 tcggtttcag gaaggtggaa atcagggatt cacaattcct tgtcaatggt tctgcaatat
     6241 acctgaaggg agtcaatctt cacgaacaca gtgacgtgga cggccatgta ataagtgagg
     6301 agttgatcct taaggatata ggccttatga agagcaataa tatcaatgct gtcagaacat
     6361 cacactatcc gcagcaggaa ctcttttacg aattgtgcga taaatacggc ctctatgtga
     6421 tagatgaagc gaatattgaa tcccatggaa taggatatga caaggatgta accattgccg
     6481 acaagcctga atgggcaccg gcgcatatgg ccaggatgca ggccatggtg gaaagggata
     6541 agaatcatcc ttcaattgtt atctggtccc tcggcaatga atcaggtgac ggtcataact
     6601 tcctggatct ttacaaatgg accaaggata gggacaaaac acgtccggtt cagtatgaaa
     6661 gggctgaaaa gtcgaccaat acaaccgaac ggcacactga tattgtatgt cccatgtatg
     6721 acagggtatg ggaggtggaa gcctnnnnnn nnnngcgaag gatcctgcca gcgaccggcc
     6781 gtatatttta tgcgaatatg cccattcaat gggtaatgca accggaaacc tgcaggatta
     6841 ctgggatgtt atagagaaat atcccatcct ccagggggga ttcatttggg actgggttga
     6901 ccagggtatt cttcagactg atgaaaatgg tgaaaaatac tgggcctatg gcggggattt
     6961 cggcgaagaa ggcgtaccca gcgacggcaa tttctgcata aacggcctcg tactgccgga
     7021 cagaagcgtg aaacccggac tgaatgaagt gaaaaaggtt tatcagtatg tcggctttga
     7081 gtctgtggat cttaagaaag gattgataag aattaaaaac aagtatgatt tcaccagtct
     7141 ttcaaagttt gaccttgact gggaaataat ggcagacgga tccgtgatac agtcgggaag
     7201 agcgcccctt tctgatctgc agccgggtaa aacagcagat attgcaatac cgctgcagat
     7261 gccggagcct ttgcccggaa cggaatactt cctgaatttg agaatatccg ggagcgaagc
     7321 atggggtata ataccggagg accatgttta tgccacggaa cagttcaaac ttcccgctga
     7381 aggtgctccg gtaccgctga aacagtctga tctggctgtg cttcagatca ccacaaaaga
     7441 gtttctgctt gaaatcgggg gcagtgatat gttgctgaca ttcaaccttg agaccggcag
     7501 acttgaatcc tacaaataca ggggaaggga gttgctgctg aagggtccgg agccggattt
     7561 ctggaggccg cctaccgata gcgattatgg ctacaatatg gacaggaaac tgggtgtctg
     7621 gaaaaaggcc ggagaaaggt ccattttaaa aagtgttgat atcaggcagc cggaactggg
     7681 cagggtgctt gtggttttca gatatgatat tccggatact gagggcaaaa agatagctgg
     7741 ttactcctca gcatttaccg tgcttggttc cggggatgtg atcatttcaa actcatttgc
     7801 aaaggtatct gatcagattc ccgaaatacc aaggatgggt atgcagatgc agctccctgt
     7861 tgaattaaca aatctgaagt ggttcggacg ggggcctcat gagaattatt ccgaccggaa
     7921 gacatcggca gaaacaggat tgtatgaaag tacggtcgcc gaccagtatg tcccgtatat
     7981 ccggccgcag gagaatggtt ataaaacgga tgtccgctgg ctcagcctta ccgataatga
     8041 cgggaacggt atccttgtaa gcggaaatcc cctgttctgt ttcgcggcac ttaacaatat
     8101 ccacgatgat tttgaatcgc ccggcaagct gagccagtac aggaaagatg caaaaagcgc
     8161 caatacccat acaaatgacg tcaaaccccg tgacttcgta aatctgaatg ttgatctcgc
     8221 acagatgggc ctgggaggag ataatgcctg gggtgccgag atatacaggc agtacaggct
     8281 tcttggaagc aaatatgaat atagtttccg gatccgtccc gttgtaaggg gagatgatgt
     8341 tataaaactc gcaaaaggga gatattaggt aaactatttt ataggttcgc cttcagtatt
     8401 tcagttctac tttcaggact tcgctttctc ccgtgattat atttcttaca ttatatatca
     8461 ggggattggt attggatttg cttaagacaa ccctggtagc tgaatttgcc ggaagagtta
     8521 ttgatgaagg agttcccggc accccatctg taaggtaaaa gggaatatcc gatctgttgg
     8581 ttatttcaag gtaaatgctt ttctcattct ggtaatacgg tttcccgacg gatatgcact
     8641 ggtggaagaa tggcagggca tattcctttc tgcctgccag catgtcatca aaataggcaa
     8701 gggtacggcc tgcgaacatg gcttccttca gtgaatcatg gctgcgttcc ctggcaaaga
     8761 ccagtgtcat gggcctgtgt gtttttcctg catattcctc actgatcgca ccgtgcatgt
     8821 cgctgttggc aaatacagcc agattattct ccagcgcgaa tgtcatcacc agcggataat
     8881 actccttgtg attgaagaac tcaataccat gaagccactt gttcctgata agtctgtgat
     8941 gaatattgta cattctggga ataccgtcag gctgctgcgc tttccatccg ggatggttcc
     9001 agagaagaaa tcctccctgt ttaatggtgg cttcaaatgc atcccatacc gagtcaacag
     9061 caagggcatt ggcatctgaa atgaataagg cattcacatg tccgggcggc attgaacggg
     9121 ttatttcggc tccgtgcacg agaatcaggt tataatccct ggcagtgttc cgggttattt
     9181 tccaggcagc attatgatca acaggaatat aatctttatg cggggtatat tccaggtgat
     9241 ctgttattgc aatggcatcc agtccgtccc tgtaggcttc attcactctg atggtcggcc
     9301 atacgtttcc gtcagagaaa gcagtatgga gatggaaatc acatttcagg gtaacatatc
     9361 ccggaagatc cggaaggttg ataatttttc gctgtccggt aagcagtaat gttgtggaaa
     9421 taatcagcaa aaggaaaaca gacaatcttt tcataactgg ttttttattt tgtgtaatat
     9481 ggaaacatgt tgcgggccgg cagaaagtat tttctgagcg ctgaccggaa aaatgttttc
     9541 aacatggcaa tcagtgtcag gttttattta aaaacaggag gcatgaatca ttcttcagtg
     9601 tcagttccct gtatttcttc ataaaaatat gcaattttcc tgtcgaaatc gtcccatgta
     9661 tatatctttt tcttcctgtt gaagaaatag aaattcagca ttgcaaaatc accggcgcac
     9721 attgttgcat gtacgaacag caggagtgac atgctccact tccagagacc gggcagaatg
     9781 aaaacaagaa ggatcagcgc aaggctgata aatacgaagg gtgccagtgc aaccagtctg
     9841 aactttttct gaccggttac ataacggtgt gccgtgacat aaaacatata ctgccggaga
     9901 tccattccca ctcttatcct ccttgctcct gatattaaat agacaataat atgtaccagt
     9961 tcatgaaagg gtatcagaag cagcggaaac actatcagac caagaaatgt gtgcaaggcg
    10021 atgctcatca aggggaaata gcctgcaata ttgattctga tggtaatggc aaaacccagg
    10081 aaaataatac agaccgccca gaaaaagacc ataacctccg atttccttcg aatatagtct
    10141 acaacgaaat cgatcagctc ctcataaggt aatttaagca gttccctgta tcttgacgga
    10201 tcttcaagat cctccacctg caagcccata aaattgattt tagcaaattt aactttttgc
    10261 ggaagaatat aaaagaaaag gccgacacgt ttattgattc cggaatccgg aagactgccc
    10321 tggttgattc gaagataaaa ccggtttcaa tgattgtttt tgttatagga caaggagtat
    10381 taccgcgctt gaccctgccg gccgggagac agatcagacg ataagggaag gtgttcttat
    10441 tgtaaggaag ccggttcctg aggagatccg gatatcagag ctgccacctg cgagtaagag
    10501 ggcaagagtc aggcgggata aaaaccctta tcatcaataa acagaaaagt gaagcctgag
    10561 cttcactttt caaaagtgta ttctgtcctg agccgggaac aaaaaccaac ccggaacggt
    10621 ccggcggctt cggttcgtta ctttttagcg tctgataatt agcttctgta taaagtgctg
    10681 actgcctgat tcagctctta atgtataaat cccgtttggc agatcaacgg acaagggaat
    10741 gatattgcca ttcaattcat tggaataaac gatccgcccg ttcaaatcgg taatggttac
    10801 tttaacagaa cctctctgaa gatcatcaaa atcaatcaca aaatttccct cactgggatt
    10861 cggatacatt ttgaaccagg gcacgatctc ttctccaatt gcagttacat tcagagtaaa
    10921 cgcatgcgta tggatagtgc tccacctgtt tgcttcatcc ctggcccgca tatataaaat
    10981 atggcttccg ctgctcagtc ctgtcaaatc cgcaataaat tccagagtca ccctgtcggc
    11041 cggtacccga actgcaacct tcgttccctt gccaaagccg ggatcattat caatgaaata
    11101 ttccagtccg gtaattttcg gggaagcagc aggtactctc ctgaagagat aaaataattg
    11161 ctcgtgtttc atgctccacg ccccattgtc gtcgcgggcc ctcactccta tcatatggaa
    11221 tccctgagaa agtccggata agttggccag gaaatttaac gacacatctt ttgccggtgt
    11281 gcttacggga atggtcactg ccttgccgaa tcccggatca ctgtcgataa agtattccgc
    11341 cttatttatc ttctgtccaa aacacaattg aacagacaga ataaacacaa taaataaagg
    11401 tagctttttc attgttatcc ctgttttgat gttacttacc tgctttgatc ttaataatgc
    11461 atgaaagacc cttatcggaa taggcagtac cggctattgc agcttcgtat atacgcggta
    11521 tgggtggcag gcccgataaa acatagggag ttgctccgcc aaatactccg cagtcgacac
    11581 cggtgacacc tgccccgatg gcgggagaac cggctttcaa cttgtatctt gcatcctgac
    11641 tgtaaccaag ggttccgtcg ggatcaacaa atacatctgt catatcgatg ttgtatttgt
    11701 tcccattggc atcggtgccg tcataggaaa aaatattgtt gattattgta ttgccggtat
    11761 tttcctgcat ttttcttgtt ggatgtataa atatattatt ggagatggtt gaattgtaaa
    11821 caccagataa aacataagtt tgggttcctc ctatgaaaac attatttgta atctgcagag
    11881 ggtagctcgc cttacttaca ctgatgtaat tggtgatatt attcgaaata accgaattgg
    11941 tcagattaac ataataagta gaaatatcag aaataaaatt ttgggttatc gtaatattgt
    12001 tactgttcga atatatctgc aatttgttaa tcttacatct tgatacgaca atttcatcat
    12061 cgcgaatata aatagtctgg tctattatta aaccacatac cttgcttccc tctgaacctt
    12121 taatgaaatc aagactggca cttatttcag caacatggat attagcctga gtactgtcat
    12181 tatcagcaag gaaataaccc ggaccgatta ttacaagttt tttatcgatt ttcgcaccgg
    12241 cataattaac aggtgaacct tcgacataaa ttgtatctcc gggttgggca ccgtcaattg
    12301 ctgactgaat tgttgtataa tccgcattgg cagctgggtc gttgttcacc ctgataagat
    12361 gctgtccaaa gataattccg caaaatagcg agaatagggt gctgagtaaa agtaatttct
    12421 tcataattag atgtattaga tggtttatag ttaaaagaag atggctcttc taccgcaata
    12481 taaaattacg ctaaaaaaag aacatgggat atcaaaaaat caggaaattt ttattgattt
    12541 tcaattaact atattttttt ctgtcagagc ttcggccccg cggtagtcaa agtaccgctt
    12601 tcaggcaaaa agagcttcat gcataatcaa atggtccgta atttgataaa tttgcatata
    12661 atggcgactg atgtgagaca tttctttttg ctgatagtat tcataattct tcctgttccg
    12721 ctttccggcc agacggatac gagccgtaag gttaacgaaa gaacagtcat acctgattca
    12781 gcccgggaag agatacatgt tttgcaaaag gttgagagaa acggcctggt ccttcccgag
    12841 gttgaaataa aggaagttac gataatcggc cgccggcagg agagctacaa tccaagaaag
    12901 atcagggcac aggaaagaca gtatgacagg cttgtttaca accttaaaaa aacttatccc
    12961 tatgccgtga taaccaggga aaaactatct gaggtcaata cccggctgga gcaaatcagc
    13021 gatgaaaagg agaggaagaa gtttctcaaa gattttgaaa aggaattgtt tgcagaattt
    13081 gaggcggatg tgagaaatat gaccattacc cagggaagac ttctgatcaa gctgattgac
    13141 cgggaaacac agaatacatc ctatgaactg ataagacaat accgcggatt cctgtcggcc
    13201 gttttctggc agggaatagc aaggatattc ggatccaatc tcaaggcgga atacgatccc
    13261 ttcggggaag atgcccttat tgaattgatc gtgagggata ttgaaatggg gtggttgtag
    13321 tatggcgctt atccggacag tgtttgaatt atagtcagta cttaaaccgg aaataaagca
    13381 ggttttcgga ttttcacaaa aagcggtcag gatgaattat cagggacagg gattgttgct
    13441 ttttgcggct ggcttgtgcc cggcaaaaaa aactgagccg ggacaaatac ctcaatataa
    13501 tattcaatgc tatgcgaatc tcagcatgct gtaaatcaaa atcatattat gatgaaaaga
    13561 attttcactg caataaaaat cattcctgag aaatcttttc tggatattca ttcctctttt
    13621 caggcaattc tggcaaacga gaaaatcaaa tgggttgata cttccaatat tcatcttacc
    13681 ctggcttttc tcggggatat tgatgaaaaa agaattcctg aactggccgt catgctgaaa
    13741 cttaaatgtg cgggatcggg ggagtttgat ttcaccctgg cgggggccgg tatttttaag
    13801 aatctcaggg attccagggt tatctggctg ggtgtcaaag agtacgaaaa gctgatcagt
    13861 ctttatgaag aaataaaggc aggattgaaa gagactggct ttcaaacaga agatcgtcag
    13921 ttcagacctc atattacgat tggaaggatt aaattcatcc gggatgccgg taacctcgga
    13981 aaggccctgg agatgtacag ggatcagttc atacagaaag tgaatgtgga ggagcttgta
    14041 ttgtatgaaa gtattctgaa accttcaggc ccggtctaca aagccctgga aaaattcagg
    14101 ctggtgtagt tgttgccata aaattcaccc aaacaggctt aataatgttg caggttcatc
    14161 atgtttttaa tcaatctttt atacaacgaa ctgatcctcc ccacaacaca gtattggatc
    14221 ctattggtaa atcagaagct atatagcctt gggcccgata ccatgcagat gttgatttat
    14281 ttccagttga actccacaat aaattacttt ccctgatata tacaaatgac ccatcgtctg
    14341 cacgaaagcc tccgggcaga ccagtaaatc cacttgaatt tgttgctccg acattaggac
    14401 tcttccagta atcagttcca gcagccttca tctttccacc agcaacatca ggcccaccta
    14461 aaaaagtctc aataatcttc cattccttaa ggtcaggtac atgccatcct gaagggcaca
    14521 gttttcctgt ttgtaccgtg taccagttat atagagcgcc atagatgtct ttattaatca
    14581 cgtcattatt gtaccagcaa taagccggct tgaaaagctg actccattcc cggttaccgg
    14641 ttatcagcgg aatgcttgtc ccatcattat acctggttgt tttcaggttc tcagctatcc
    14701 atatctgtga accaattcct atggtctgat atatgtttcc gtctgaatca gctactgtgc
    14761 ctttctctcc tgtcagatct gcgggtgtgg caaataataa ttcattcccg tatgaagtgc
    14821 ctgcctcact tgtagcgtaa gccctgacgt aataggtaat ttcaggtttt agaccgccaa
    14881 tcctgagttc aaagggtaca aggtagggat aaagatgggt gctgaccgta ctgtcttcaa
    14941 ttgtgggcga aggagagtcg ctccagcaca agcctttttc aatcagtttt gtactgccgg
    15001 cagatattac aactccagtg caggaagcag atgttggtgt gacaacagat acttcagttg
    15061 tatctacttc aggtaaaaca gatttttctt ttttacaact gatcccgagt acaataaaga
    15121 acattgatat acttaccacg caggctgtaa cacttttatt tgcgtctttc attgtcttta
    15181 aatatcagtt tttatcttaa tggcttattt ttatcttata agaataatga agattgcgaa
    15241 ttcagtggca atgtcagaaa attaagacag cttacaaata atatcttttt cataagcagg
    15301 aattattaaa atggatttga cctcagttca gttttatgca gacaattttt ccattaaact
    15361 gaacttgtta tcaaaattat gaaataatca aacctgagtc aaaaaaatat ccgcgttgtg
    15421 gagatttgaa atttgccgtt ctcttttaag ttgggtaaac gaaggttctc atactttgcc
    15481 cgatgatttg tatattgagg cacctgattg caccaaagtg aaatatttaa aagtatttca
    15541 ggatattttc aaccatactg caattaatta tcaaatgtcc agtttatttt ataatttact
    15601 ggacaatttt attacttttg caaaagcaat ttattaatga aaacatctga atacatatca
    15661 cttacaatcg acagacttcc gaaaggatat gttttcacct atgccgactt tattaccgag
    15721 gtgaacaaaa aagaagccgt tataaaagca cttaaccgga tggtggcttc cggtaagata
    15781 atgaaactcg ccaaaggtaa atattataaa cccgaaacca caccgttcgg aaaactgttg
    15841 cccgaccaaa aacaggtagt aaaagacttg ttggaagaaa acggtaaaat tatcggttac
    15901 ctgacaggtt acagcatata caatcaattg ggtttaacca cgcaggttag caataccata
    15961 caaatagggc gaaaccaaat aaggccaagt tttaaacgcg aacgttatac catttcattt
    16021 atcaaacaaa aaaacaccat cacaaaagaa aatattccct tattacaaat tctggatgcc
    16081 ttacggtaca taaaaaaaat acctgatgca aaaatagaat catcgtgtcg aaggctttta
    16141 aatatcttaa aagaattaac cgaaaacgac aagagttcat tggttcggtt ggcgttaaaa
    16201 tatccgccgg ttacaagggc tttattagga gctatgttgg agcaattgca gcaaaccgaa
    16261 ataacacaac ccttgcttaa atcgctcaat cctattacta aatacaagct ttcgggcatt
    16321 tcgaaggttt taattaccgc cgaaaaatgg aatctgttat gaagctgcac cacgatataa
    16381 aattattttc ggacacattg agggctgcat cacagcactt ggatattaaa ttggaatttg
    16441 tagaaaaaga ttactggata accttggtgt taagtcgatt ggcaaaaaat aaatatgttg
    16501 atgaatcggt ttttaaagga ggtacatcgc tatcaaaagg atataatctg ataaagcgat
    16561 tttcggagga tgttgatatt gccattataa acgacaaggg taaaacaggt aatgaaatta
    16621 agaccatcat ccgcactatt gaaaaagaaa tcacatccga tttaaccgaa gtgcaaatgg
    16681 acggtgttac cagcaaaggt tcacggatca gaaaatctgt ttttgaatat gtgaccacag
    16741 ataaaagtaa taagaacaac aaattgattg tcgagataaa ttcatttgcc aatccttttc
    16801 cttttcaacg gctttcctta aaaagttttg tatttgactt cttacagcag acaggaaatg
    16861 aaaaatacat agagcaatac gaccttcaac catttgaggt aaatgtattg agcaaagagc
    16921 aaacgttgtt ggagaaaaca atttcactaa tccggttttc attcgaacaa aaagccgttg
    16981 aaactatttc gaaaaagata cggcactttt acgatttata cttcttaatg aaaaatcctg
    17041 aatgtgctga atttgtttca tctgattcgt ttaagaaaca atttgaaact atcctgcaac
    17101 acgacaggga aatgttcgaa gaaccgatag gatggcaaac taagttaatt tcagaatctc
    17161 ctttagtggc agatttccca gccatctggg aacaattaaa agaaaaatat caaactgaat
    17221 tatcagcgtt ggcatatagg acaatccctg ctgaaaatga agtggctaaa tggtttaaag
    17281 aatttataaa gagaattgaa tgaagttaac tgaagaaaaa tcagaaaagg catttaccga
    17341 attgttattt accgtttaca gcgcctcatg tagcaggtgt tgcggcatta atattaccac
    17401 aaaaaacagt ttatgcagcc ttatcgccgc caccgctttt cga
//
LOCUS       DAOSPX010000027        17415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3397_length_17415_cov_4.176498, whole genome shotgun sequence.
ACCESSION   DAOSPX010000027 DAOSPX010000000
VERSION     DAOSPX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17415
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3397_length_17415_cov_4.176498"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLZ75_02570"
     CDS             complement(<1..885)
                     /locus_tag="PLZ75_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 9"
                     /protein_id="HOS71314.1"
                     /translation="MRKFLFLFLVAFPFITVFSQIDDKLISDIKNTGYIHRPLPLDHS
                     KSYETFAVTKKVLVSEMLCDMEDLSKWTHSGVGGMRLTSERSVSGKRSLRLVAPTVPE
                     VHPGWGLGRGTSMASFDMGGRNIEKFNRVRLHVYPDCEGARSIYLNLVFENDGKIKVP
                     DPFGREGIHEMNLVNGTWNECVLEIPELARDKVTKISLAIEIFGREQTMGDSLKFDID
                     AIELQVVEKPEIASGWIPADDRIIFSTTGYGLNSDKTAIVQTNDNVPLFQLVDKFSAQ
                     VVYEGKVVPTKTPLGSFKV"
     gene            1201..1578
                     /locus_tag="PLZ75_02575"
     CDS             1201..1578
                     /locus_tag="PLZ75_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS71315.1"
                     /translation="MQNKVSFTDIKACPLRYVLAINDTLNVLTGKWKLPIIGSLLSDR
                     KRFTEIQQTIPRITPRMLSKELKELEINGMIKRTVRNTSPVTVEYELTESAVQFREVL
                     DKMLQWGLQHRDSVFSQENQDQA"
     gene            complement(1630..3156)
                     /locus_tag="PLZ75_02580"
     CDS             complement(1630..3156)
                     /locus_tag="PLZ75_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS71316.1"
                     /translation="MKRRKFVKSVGALSAAAYTYPNKIIDETSSLFYHGKLLEAPSVF
                     FDQDISNSGGPEDGPNQPMGRPQGVKPGYVVWAWNPAATNPDCKNVIENNNWYFNPEN
                     FDQKIINGMFAESIRELTGEKDLKKAWDLIFRYHNKRKHNTDRGYTAGEKIYIKINQG
                     TSDWLLSREDKNNGYFVPSYPVKPGTRTRGYGAMETGPYIVPELLRELVNECGINQAD
                     ISIGDPHKHIFGHNYTYWHREFPNVRYTDRYSEDFNRTVVRETENVLLFYSDKTQDDK
                     LFDVIEEADYMINLANFKPHGSAGITLTAKNHFGSIAGRAARLHYSNVAPSWETSPRQ
                     VGNPNNRGYRKYRAMVDLMGSKYLGRNTMLYIIDGMFGGGSDETRGPVKYLMPPFNNH
                     WSSSIFMSLDQVALESVCFDFLRTEWDGVNKHDPANAIWEWVPNMPGVDDYLHQTADS
                     KNWPEGLIYDPDNSGIPLPSLGVHEHWNNPTDMQYSGNLGKPNGITLVSVGDYIVKRV
                     "
     gene            3540..5984
                     /locus_tag="PLZ75_02585"
     CDS             3540..5984
                     /locus_tag="PLZ75_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOS71317.1"
                     /translation="MKKITGIILILFFNFAFLSAQVDEKLKEDIRNTGYVHSPLPLDH
                     SKSFETFGLTKKVLVSNVLCDMESMNKWTHKGIGKIYRTGERSKDGRHSLRLAAPTTV
                     TEFLGWGLGFGTCLASYDVGGENWEKYNRLHFYIFPDCEGARSIYLNLYVENDGKIKV
                     PDRYGREGYHEINLVNGQWNECFVEMTELARDKVTKISFAIEVFGKERTMGDSLKFDV
                     DNVKLQVIENPEVVNGWMPAENRIIFSTTGYGRDSKKSAIVRVKNHGGKFQLIDYNTY
                     QVAYEGNITPVKTSIGNFETIDFSDFTREGRYILRAGDVNTMPFYINRNIWEDSAWRV
                     LNFMFVERCGYPVPEKHGVCHTDLNGEHNGKMFAVNGGWHDAADMSQQTLQTGEIAFA
                     IFEMANRAKEKGNTDLYLRLMEEAQWGLDYILKTRFGDGYRVQTWGTNLWTDGFIGTF
                     DDAGRRRVRVHNRAFENFVFSGIEAYASMSIEKDVMLKQHLAKVAEEDFQFAMERYKE
                     LGYEELLGAGGGGDHAAMASRSQYNANISFAASLLYKLTGKKYYADIAAEYIKYTIQC
                     QRTEPLKDKDKIRGFFYRDLNRKSIVHYTHQSRDHVYMQALTALCETQPDHPDYKTWA
                     GSIALYAEYLKKIMQYVQPYGMVPSGVYHKDEIKDSVNFYQIQVGIRGGAEKDFREQL
                     ENGVKLDKEHYLRVFPVWFSFKGNAAVHLATGKNAALCGKFLNDKELIDIAEQQLFWI
                     VGKNPFGQSLIWGEGSNYAQQYTALPGETVGEMPVGMQSRFNEDMPYWPQFNTATYKE
                     VWGSSAGRWFSLIAEF"
     gene            6010..7242
                     /locus_tag="PLZ75_02590"
     CDS             6010..7242
                     /locus_tag="PLZ75_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOS71318.1"
                     /translation="MNNTLKADFTQRDAGIDFLRALTMFIMIFVNDFWKIHDVPHWLE
                     HAAFGEDFMGLADVVFPAFLFVVGMSIPFAIERRYSKGLSAESTIGHILTRTFALLVM
                     GAFITNSEARLSPDVSYKIGVYWILMVTAFLLVWNNYPRTDSRNRKILFSILKTAGVI
                     ILFYLAVTFRNPKGGVFAAHWGILGSIGWAYLICAMIYIFTRDRLKYLIPVWFAFVII
                     CILGSRMNETWGGTAILQFPRPNFYNDMLNVLHIGNGALVSFTMGGLIFSLINAKYLN
                     HDNRKKMIYLISSVIIFVLAGYISRKFWILSKISATPPWIFYVTAIVIVVYTLLKCLA
                     DIGKAGWLNIIKPAGTATLTCYMIPYVSYGLADLTGIVLPDWFTHGFMGLVNCFCFAL
                     LIIGITWLLEKINIKLKI"
     gene            7277..8851
                     /locus_tag="PLZ75_02595"
     CDS             7277..8851
                     /locus_tag="PLZ75_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOS71319.1"
                     /translation="MKFFKLFAIAFVFAAFTACTGTKKTQEKEYPMFWSWLDYRPGMN
                     MDSVCAIMNETGLDGLILNAPTPDDYRVAIPTVNKYGIKVFAWLWTMNLEHDRDKIVK
                     EHPEWLSVNRKGESLADTRAYVDYYKFMCPALPEVRDHIRQKIKAYCEVDGLDGIAID
                     YHRFVDVILPTTLWPRYGIVQDREYPEWDFGYHPEMIRLFREKFGYNPLDLEDPSADE
                     KWQQFRCDQITEVANEIAEVVHSYGKVMAASPFPTPAMSRKMVRQDWGRWNLDVVFPM
                     VYHTFYTGDVGFIEDCTIDNVKQKNSMTTLYCGMTASNGPEMFKCMDAALNNGAEGIA
                     IFTVSSLRSPEVRARFKEYADSVRALRAETKVNPAATGKTEPNRNPFDNKNIMNGVDM
                     RMKAYLSLANALKSPKMRQYDRNEVERIAVNAMQVNTPEDYLNRLTRPMFPRMPIRPE
                     ATIIADFFRDDMNKELNLGEYKLVDEYDITKIYEVTDANSMVTFTVRFYLYGGILSGW
                     SVEPVKASYDKYRARI"
     gene            9114..9602
                     /locus_tag="PLZ75_02600"
     CDS             9114..9602
                     /locus_tag="PLZ75_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71320.1"
                     /translation="MKIVKWTFLLLTAMLMFSCSKKQEEEENQNFTVVTGEWINIFGQ
                     NVVMEDHPLLGGLCIVADEKVNEKTVVNGTAHIYLRSAISKVPAVYSYVPGEFGKNNS
                     VELKGMLDKGYVLKFGTLPEASPLGTLHVKGLIIPKGAKLPAKLDIKDYEQVKEHFEL
                     KD"
     gene            9908..10537
                     /locus_tag="PLZ75_02605"
     CDS             9908..10537
                     /locus_tag="PLZ75_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOS71321.1"
                     /translation="MKIRDIFEHNEKWIAERLRADPDYFVKLSGPQKPEYLFIGCSDS
                     RVSVESLLGTKPGDVFMHRNIGNVIPSNDLNALSVISYAVNHIRIKHIIVCGHYNCGG
                     VKAAMTARDEGLLNPWLRHIRDVYRIHRNELNSIDTPGRRFDRLVELNVVEQCINIVK
                     TRDVQKALLEKRISLHGWVFDLRTGRLIDLKINLEKIMDEIMDVYNLFE"
     gene            complement(10570..12903)
                     /locus_tag="PLZ75_02610"
     CDS             complement(10570..12903)
                     /locus_tag="PLZ75_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOS71322.1"
                     /translation="MNTIRKSFIITSAAILLILLAGGCARKWSEEKLESHTLIHNKGG
                     LTLGYSPSSGVKIIAADRFAFKDLNRNGKLDVYEDWRLPFDDRARDLASQMTVEQIAG
                     LMLYSSHQSIPAAGRAPAGGGTYGGKPYAESGAGAGDLSDQQKIFLSDDNVRHVLITS
                     VQSPEAAAVWNNNAQSFVEGIAFGIPVNTSSDPRHGSDPHAEYNAGAGGNISLWPGTL
                     GIAATFDPSVMKRFGEIASAEYRALGIATALSPQIDLASEPRWSRFEGTMGEDPALAA
                     DMARAYVDGFQTSSGPDEISGGWGYQSVNAMVKHWPGGGPEEGGRDGHFGYGSYAVYP
                     GNNLRDHLIPFTEGVFKLEGPTAMASAVMPYYTISYDQDTIYGENVGNAYSKYIVNDL
                     LRGVYKYDGVVCTDWGVTGNVSSIDRFEGKPWGAETLSEAERHYRIIMAGLDQFGGNN
                     AKGPVIEAYNIGVKENGEEFMRKRLEESAVRLLRNIFRAGLFENPYLDPQKTKSIVGS
                     PEFMKAGYEAQLKSVVMLKNTAKTLPLKKNLKVFIPQKYVPAGRNWFGQTTPEKWTDP
                     LNLNIVTNYFGIVNDPSEADFALVSITSPSGGTGYSTDDLKAKGNGYVPIPLQYSRYT
                     AVHARAASIAGGSPFETFTNRSYKGKTVTANNSYDMKMVNETRAQMGNKPVIVLINVS
                     NPMVFSEIEKSASSILIHFGVQDQALLDLITGAEEPSGLLPFQMPADMRTVEEQNEDV
                     PRDMKCHTDSEGNVYDFGFGLNWKGIIKDSRTARYGN"
     gene            13347..16562
                     /locus_tag="PLZ75_02615"
     CDS             13347..16562
                     /locus_tag="PLZ75_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS71323.1"
                     /translation="MRKKLLMFFTIFLMICASALAQERTISGMITSAEDGSALPGVNV
                     RVQGSSIGTISDAQGRYSIRVPSGFNTIEFSFIGMESRVVTIGISDNIDIAMESSSQL
                     LDEVVVTALGIERKAKAIGFAQQELKSDQLSAARETNLTSFLTAKVAGVRVSKTASGT
                     GGSSAITIRGAKSLLGNNQPLFVLDGVPITNIGNTSGGTWEGLDLGDGMSDLNPEDIE
                     SMQVLKGPNASALYGSRGSNGVILITTKSGRQKKGIGVELNSNFSLETLNLFPTYQNY
                     YATGYEDTNLYGSFVEIPEGSGKFYETMDTWHGDSWGPPMDGRRVIVDPFMFPDEKPF
                     SRTFTLLPQPANNVREFYNKGIGLSNTLAISASDEKTSARLSVGHGSNTGIIPNSKWQ
                     KQTIVLNVNSRVTDYLSFDGKISYLRDEGNNRPILGTMYQGGNVSQVFAVMGRYVPMD
                     FLKEYYERTKSPGTFPAVQYNPYYIVNELKSRDVKDRIIGQISTTLKITDWLSLMGRA
                     GTDYYTLKLVRTWPVGSKISDNTRGRVYNEISLVKDINADIILTATKELSSQISGNLS
                     VGSSILHQERNNQSIDGRVFKAPGVYDVSNCTDIRPYTWLSRKQMQSVFFTGQLAYNN
                     YLFLDVTGRNDWSSALGINNYSFFYPSVSTSFVFTDAFPSLAGNVLTFGKVRASWAQV
                     GNDSDPYLTRNGYASTTTTYAGQPISWMNSTIPLFDLKNELTASWEIGTNLRFLNNRI
                     DFDFTYYDGKTTNQILPISISNSSGYSDVVINAGEIQNKGIEIALNLNPVRTSTGFSW
                     DINANYSRNRSMVVSLAPGIETLVVGGGASATIEARPGQPYGNIVGYPYMRAPDGQKI
                     VDETGSYLFDPQLTVLGNITPDWIGGLNNTFSFKGFSMNVLLDFVQGNEITSVTKYQM
                     EAKGTGKWTVEGRRLRDMDDEGNQLPLVGILDGVVEIDDGEGNITYEKNTKAVDGQTY
                     WAMRAWGSISEEFVMDGSYISLREVMLSYSFSPSRLAKTPFAGITLSLIGRNLLYLEE
                     HMMGMGVSPESSPNTSAGWAGTEYFAMPTTRTWGFNVKLTF"
     gene            16587..>17415
                     /locus_tag="PLZ75_02620"
     CDS             16587..>17415
                     /locus_tag="PLZ75_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOS71324.1"
                     /translation="MKKSFISITTFLLALVLLISNACTKNYEEMNTDPKIVTADIIDP
                     GLLLTYVQSQAIVIGGSYGNSADGSLSGMCTRGDGSPFGEADMPGMWNFSYTVTLNNL
                     SDIIHIIEKRENRDELVNKTAIARIMKAWSVSRLTDTYGDVPYSESCLPQEQAIYSPK
                     YDSQESIYRDLFKELKEAVAQMDPSKPSFGSADLIYGGDVGKWEKLANSLRLRLALRI
                     RYADPDLARAQLSDLTEADLMSDLGDDAYILNSTEYPEHLNPRYFRIINWGTVSEEVH
                     "
BASE COUNT         4778 a         3765 c         3996 g         4876 t
ORIGIN      
        1 aactttaaaa ctccccagcg gggtctttgt tggaacaacc ttgccctcat agacaacctg
       61 tgcggaaaat ttgtcaacca gctggaacag cggaacattg tcattcgttt gtacaattgc
      121 cgttttatcg ctgttcaatc cgtatcctgt ggttgaaaag attatcctgt catccgccgg
      181 tatccatccg ctggcaattt ccggtttttc caccacctgc agttcaatag catctatgtc
      241 aaatttcagg gaatcgccca ttgtctgttc cctgccgaaa atttcaattg caagagaaat
      301 ttttgtaacc ttgtcgcggg ccagctcagg tatttcaaga acacactcat tccatgttcc
      361 gttgacaagg ttcatttcat ggattccctc cctgccaaag ggatcgggaa ccttgatttt
      421 accgtcattt tcaaaaacaa gattcagata gatggatcta gctccttcac agtcaggata
      481 aacatgaagc cttaccctgt tgaatttttc aatattcctc ccccccatat caaagctggc
      541 catagatgtg cccctgccaa ggccccagcc cgggtgtact tccggtacgg tgggagccac
      601 gagcctgaga ctgcgtttgc ctgaaacgga tctttcagag gtcagcctca tgccacctac
      661 tcctgagtgg gtccatttgc tcaaatcttc catgtcgcac agcatttccg aaacaagtac
      721 cttttttgtg actgcgaaag tttcatacga tttgctgtga tccaatggca aaggcctgtg
      781 tatgtatcct gtatttttaa tatcagagat cagcttatcg tctatttgtg aaaaaaccgt
      841 aataaacggg aaagcaacaa gaaataaaaa aagaaatttt ctcatggtta tgtatagttt
      901 tggtttgtgt tttacaagtg tccgaaatta gtgaatctta tttacagcgt caatagtttc
      961 acacccgaat gccatgcatt cctgcctttt cagattgtca aattcttcca tgccttcaga
     1021 tatccacagt gatgtgtcgc gtgagagtcc atttttttat ttgtgttatt tatatgaata
     1081 tcaatatttt aattttgccg ggttttatat caaaaatata ttaagtttgc tatggatttt
     1141 atagtactat acttctgtat actgaccaat gcacggaatt aaccagaatt aagatctgct
     1201 atgcaaaaca aagtgagttt taccgatata aaagcatgtc cgttgagata tgtcctggct
     1261 ataaatgata cattgaatgt acttacaggt aaatggaaac tgcctattat aggatctctt
     1321 ctttctgaca ggaagcgttt taccgagatt cagcaaacta ttcccaggat aacaccgcgg
     1381 atgttgtcaa aggagcttaa ggagcttgaa atcaacggga tgataaaaag aactgtccgt
     1441 aatacaagcc ccgtgacggt ggagtatgaa cttaccgaat cagcggtgca gttcagggag
     1501 gttcttgata aaatgcttca atggggactt cagcacaggg attctgtttt ctcgcaggaa
     1561 aatcaggatc aggcatgaat gacctgaaat atccggaaat ccctgtaaat ggtttctgcc
     1621 tccttatttt cagactcttt ttacaatata gtctcccacg cttaccagtg tgatcccgtt
     1681 tggttttcca agatttcctg aatactgcat gtctgtcgga ttgttccagt gttcatggac
     1741 acccaggcta ggcagtggta ttccgctgtt gtccggatca tatatcagtc cttcgggcca
     1801 gttttttgag tctgccgtct ggtggaggta gtcatcgact cccggcatgt ttggcaccca
     1861 ttcccagatg gcattcgccg gatcatgctt gttcaccccg tcccattctg ttctgaggaa
     1921 gtcaaagcat acggattcca gtgccacctg gtcgagcgac atgaatattg atgagctcca
     1981 gtggttgttg aacggcggca tgaggtattt caccggtcct cttgtttcat ctgatcctcc
     2041 cccgaacata ccgtctatta tatacagcat agtgtttcgt cccaggtatt tgcttcccat
     2101 caggtccacc attgccctgt attttctgta tcccctgttg ttcggatttc ccacctgcct
     2161 gggagatgtt tcccatgatg gtgccacgtt ggaatagtgc agcctggcag ctcttcctgc
     2221 tatagatccg aaatggtttt ttgccgtgag tgttattcct gctgaaccat gaggtttgaa
     2281 gtttgcaagg ttaatcatgt aatcagcctc ttcaatcaca tcgaagagtt tgtcgtcctg
     2341 tgttttgtct gaatagaaca ggagtacgtt ttcagtttcc ctcaccacgg tccggttgaa
     2401 atcctctgaa tacctgtctg tataccttac atttggaaac tccctgtgcc agtatgtgta
     2461 gttatgcccg aatatatgct tgtgcgggtc tcctattgat atgtccgcct ggtttattcc
     2521 gcattcattt accagttccc gcagcagttc cggcactatg tatggtcctg tttccattgc
     2581 tccgtatcct cttgtccggg ttcccggctt gacaggatag cttggtacaa agtatccgtt
     2641 gtttttgtct tccctgctca gcagccagtc ggaagttccc tggtttattt ttatgtatat
     2701 tttttctccc gctgtgtatc ccctgtctgt gttgtgcttt ctcttgttat gataccggaa
     2761 tatcaggtcc catgcctttt taagatcctt ttcacctgtc agttccctga ttgattcggc
     2821 aaacattccg ttaataattt tctggtcgaa gttttcagga ttaaagtacc agttgttgtt
     2881 ttcaatcaca ttcttgcagt ccggatttgt tgcagccggg ttccatgccc atactacata
     2941 acctggtttt acaccctggg gccggcccat gggctggttt ggcccgtctt ccggtccccc
     3001 gctgtttgaa atatcctgat caaaaaagac gcttggtgcc tccagcagtt ttccgtggta
     3061 aaacagggag gatgtttcgt caattatctt gtttggatag gtgtaggctg cagcactcag
     3121 tgcaccaacc gattttacaa attttcttct tttcattttt ctctttttta aaatctcaat
     3181 ccggcctgat cttcatacta cagatatatg ccatggctgc ctgccctgat aatcattcag
     3241 gggccggcca cagcatcaaa ccaggtatca aatttaacaa ttaaaactct gaatgaacgt
     3301 ggccagggcc ctggacttgt taaaaatatc taaaaggctg aatattatca ggaattagtc
     3361 cggataatat tcatatattc atggctccaa atggaataaa atccttggtt catcagcaac
     3421 tgcgacgtaa aacaaagagc cgcagaagca accggatagt tttgcatttg gccaatgctc
     3481 caataatgag cgatttaccc tttaaactat ctaaattcaa cattctaaaa gtattactca
     3541 tgaaaaaaat tacaggaata atcctcattc ttttcttcaa ttttgctttt ttatctgcac
     3601 aggtggatga aaaactgaaa gaagatatcc gtaataccgg atatgtgcac agtccgctgc
     3661 ccctggatca cagcaaatca ttcgagacat tcggactgac aaagaaggtt ctggtttcca
     3721 atgtcctctg tgacatggaa agcatgaata aatggaccca caaagggatc gggaagatct
     3781 acaggacagg tgagcgcagc aaggacggca ggcacagtct caggctggca gctccgacta
     3841 ccgtaacgga gttcctggga tggggactgg gcttcggcac ctgtcttgcc agctatgatg
     3901 tcggcgggga aaactgggaa aaatacaaca ggcttcattt ctatatcttt cccgactgtg
     3961 aaggagcccg gagtatttat ctgaaccttt atgttgaaaa cgacggcaag atcaaggtac
     4021 ctgacagata tggcagggaa ggttatcatg aaataaatct cgtcaatggt cagtggaatg
     4081 aatgtttcgt ggaaatgacc gaactggccc gtgacaaggt tacaaagata tcctttgcca
     4141 tagaggtatt cggaaaggaa cgcaccatgg gtgattccct caaattcgat gtggacaatg
     4201 tgaagctcca ggttattgaa aacccggaag ttgttaacgg atggatgcct gcagaaaacc
     4261 gcataatatt ttccaccacc ggttatggca gggatagcaa aaaatctgcc attgtcagag
     4321 tgaaaaacca tggaggaaaa ttccagctga ttgattataa tacctaccaa gttgcatacg
     4381 agggaaatat aacacccgtt aaaacatcca tcggcaattt tgaaactata gatttttctg
     4441 atttcaccag ggaaggacgc tacattctcc gtgcaggcga tgttaacaca atgccttttt
     4501 atattaaccg gaatatctgg gaggattccg catggcgcgt tttaaacttc atgtttgttg
     4561 aacgctgcgg ttatcctgtt ccggagaaac acggggtatg ccataccgac ctgaacggtg
     4621 agcacaatgg taaaatgttt gcagtcaacg gcggctggca tgatgcggcc gatatgtcac
     4681 agcagacact tcagacgggc gaaattgcct ttgccatttt tgaaatggca aaccgggcaa
     4741 aagaaaaggg aaatacagat ctttaccttc gcctgatgga agaagcccag tggggactcg
     4801 actatattct gaaaacccgt ttcggtgatg gttaccgggt tcagacctgg ggtaccaacc
     4861 tctggacgga cggctttatc ggaacatttg atgatgccgg tcgcagacgc gtcagagttc
     4921 ataaccgtgc atttgagaac tttgtatttt caggaataga ggcttatgct tcaatgagca
     4981 tagaaaaaga tgtcatgctg aaacagcatc ttgcgaaagt tgcggaggaa gattttcagt
     5041 ttgccatgga gcgttacaag gaactgggat atgaagaact cctgggggca ggaggcgggg
     5101 gagaccacgc ggctatggct tcgagaagcc agtacaatgc caatatttcc tttgcagcaa
     5161 gcctgcttta taagctgaca gggaaaaaat attatgctga cattgcggca gaatatatta
     5221 aatacaccat acaatgccag cgcaccgagc cattaaagga caaggacaag atcaggggtt
     5281 tcttttaccg cgacctgaac aggaaatcca tagtgcatta tacccaccag tcgcgtgacc
     5341 atgtctatat gcaggcactg accgcattgt gtgaaactca gccggatcat ccggattata
     5401 aaacatgggc cggctccatt gccctttacg ctgaatatct gaaaaaaatt atgcagtatg
     5461 ttcagccata cggcatggtg cccagcggag tgtatcacaa agacgaaata aaggattcgg
     5521 ttaacttcta ccagatacag gtgggaatca gaggcggtgc agaaaaggat ttcagggaac
     5581 aacttgaaaa tggagttaaa ctggacaagg aacattacct gcgtgtattc ccggtatggt
     5641 tttcatttaa ggggaatgct gctgttcacc ttgcaaccgg aaagaatgcc gctctgtgtg
     5701 gtaagttttt gaacgacaag gaactgattg acatagcaga acagcagttg ttctggatag
     5761 tgggtaaaaa cccgttcggc cagtcgctta tatgggggga aggaagcaat tatgcacagc
     5821 aatacactgc cctgcccggg gaaacagttg gagagatgcc cgtgggaatg caatccagat
     5881 tcaacgagga tatgccttac tggccccagt tcaatacggc aacatataag gaagtatggg
     5941 gatcatcagc cggaagatgg ttttcactga tagcagaatt ctgatcttgt tttgttaaaa
     6001 ctgcatatcg tgaataacac attgaaagct gatttcacac agcgggatgc cggaattgat
     6061 tttctgcgtg ccctcacaat gttcataatg atctttgtca atgatttctg gaaaatccat
     6121 gatgttcccc actggctgga acatgctgca ttcggcgaag atttcatggg actggcagat
     6181 gtggtctttc cggcatttct gttcgttgtg ggaatgtcga taccttttgc cattgaaaga
     6241 aggtacagta agggattatc ggcagaatcc acaataggcc atatattaac acggactttc
     6301 gctcttctgg taatgggagc cttcattacc aattccgaag cccgcctgtc acccgatgtg
     6361 agctataaaa tcggggttta ctggatactg atggtgacag cctttctcct tgtctggaat
     6421 aattatccgc gtacggacag cagaaacaga aaaattctct tttccattct taaaacagct
     6481 ggagtaataa tactttttta tctggctgtt acattcagga atcccaaggg tggtgtcttt
     6541 gccgcccatt ggggaatact tggatctatc ggctgggcct atctgatttg tgcaatgatc
     6601 tatatcttca cacgcgatcg tttgaagtat ctcattcctg tatggtttgc cttcgtgata
     6661 atttgtattc ttggttcccg tatgaatgaa acatgggggg gaacggccat cctgcagttt
     6721 ccgcgtccca atttctacaa tgacatgctg aatgttctgc acattgggaa tggtgcgctg
     6781 gtatcattta ctatgggagg ccttattttt tcacttatca atgcaaaata tctcaaccat
     6841 gacaacagga aaaagatgat ttacctgatt tcatctgtca tcatttttgt tctggcagga
     6901 tacatttccc gcaagttctg gattctttcc aagataagcg ccactcctcc gtggatcttt
     6961 tatgtcacgg caattgtaat tgttgtttac accctgctga aatgtcttgc agatatcggg
     7021 aaggccggct ggctgaatat aattaaaccg gctggaacag ccacacttac ctgttacatg
     7081 ataccatatg tttcctacgg attagctgat ctgacgggca ttgtgctgcc cgactggttc
     7141 acacacggat ttatgggact ggttaattgt ttctgttttg ctctattgat tatcggaatc
     7201 acctggttgc ttgaaaaaat aaatatcaaa ctgaaaatat gaatattaac ctttaaattt
     7261 ttaaataatt ataatgatga aattctttaa actatttgca attgcttttg tttttgcagc
     7321 ttttacagcc tgtaccggaa caaaaaagac ccaggaaaag gaatatccca tgttctggag
     7381 ctggctcgac tatcgccccg gaatgaatat ggattcggta tgtgcaataa tgaatgagac
     7441 gggactggac ggtcttattc tgaatgcacc gacgccggat gattaccggg ttgccatccc
     7501 gacggtaaac aagtacggca taaaggtatt cgcctggcta tggactatga atcttgagca
     7561 tgacagggat aaaatagtaa aggaacatcc ggaatggctc agcgtgaacc gtaaaggtga
     7621 aagccttgct gatacgagag cttatgttga ttattacaaa ttcatgtgtc ctgcccttcc
     7681 tgaagtgcgc gatcatataa gacaaaaaat caaagcttat tgcgaagtag atggtttgga
     7741 tggcatagct attgattatc accgttttgt tgatgttata ttaccgacaa cactgtggcc
     7801 ccgctatggt atagtgcagg acagggagta tcctgaatgg gactttggat atcatccgga
     7861 gatgatcaga ttgttcaggg aaaaattcgg gtataatcca ctggatcttg aagatccgtc
     7921 tgccgacgaa aaatggcagc aattccgctg tgatcagatt acagaagtgg caaatgaaat
     7981 tgctgaagta gtccattcct acggtaaggt aatggcagct tcaccattcc ctactccggc
     8041 tatgtcccgt aaaatggtga ggcaggactg gggcaggtgg aatctggatg tcgttttccc
     8101 gatggtttac catacctttt acacagggga tgtcggcttc attgaagact gcacaataga
     8161 taacgtaaag cagaaaaatt ccatgaccac cctttattgt ggcatgacgg caagtaatgg
     8221 ccctgaaatg tttaaatgca tggatgccgc ccttaataac ggagcagagg gaatagccat
     8281 ttttacagta agcagtctgc gttccccgga ggttcgcgcc cgttttaagg agtatgctga
     8341 cagtgtgcgg gctttacgtg cggaaacaaa agtaaatcct gctgcaacgg ggaaaacgga
     8401 acccaacaga aatccctttg acaacaagaa tatcatgaat ggtgttgata tgcggatgaa
     8461 agcctatctg agccttgcga acgccctgaa aagcccgaaa atgaggcagt atgaccgtaa
     8521 tgaagttgag cgtattgcag tgaatgccat gcaggtaaat actcccgaag actatctcaa
     8581 ccgtcttaca aggcccatgt ttccaaggat gcctataagg ccggaagcaa ccataattgc
     8641 cgatttcttc agggatgaca tgaataagga attgaacctc ggggaatata agctggttga
     8701 cgaatatgac ataaccaaaa tatatgaggt aacagatgca aacagcatgg ttacatttac
     8761 ggtaaggttc tatctgtatg gaggaatact ttcaggatgg agcgtggaac cggtgaaggc
     8821 atcctatgat aaataccgtg ccaggatttg atttgagtta tgaactgatt ttaagtccct
     8881 gactgcaagg gatttttagc agattgtaat tgcaggacat gaaattccgg ccgtcgggaa
     8941 gagttaaata agatggtaat acttgcttat tcgccttttt tctaaatcct gatcgttttg
     9001 tgtaggataa aagcaacaga tttattatcc cggaacaata acagacggta tgctgcgtta
     9061 attatcttag agttactgtc tgaacaaaca ctgtttaacc aaattgtatc attatgaaaa
     9121 ttgttaaatg gacttttctg ctccttacag ctatgctgat gttctcctgt tcgaaaaagc
     9181 aggaagaaga agaaaatcag aactttactg ttgttaccgg agagtggatc aacatcttcg
     9241 gccagaatgt tgtgatggaa gaccacccgc ttctgggggg cttatgtata gttgccgatg
     9301 aaaaggtgaa cgagaaaaca gttgtcaacg gaacagcaca catctacttg cgaagtgcga
     9361 tatctaaagt accggctgtt tattcttatg ttcccggtga attcggcaag aataacagtg
     9421 tggaacttaa aggtatgctg gataaaggct atgtactgaa atttggaacc ctgcccgaag
     9481 caagtcccct gggaacactg catgttaaag gactgattat tcccaagggt gcaaaactgc
     9541 ctgccaaact cgatattaaa gattatgagc aggttaaaga acattttgaa ttaaaggact
     9601 gaaactggcg ttccggatga gaacttgcct tccgccggat ggtgacagcc tgaagtgtca
     9661 ttattaacag gatccactga tatgaatgat taatttatat cagtggattt tttagtttga
     9721 aggacactgt tctgtaacca gactttcttt cagaataaca atgcaaagga caggcatcgg
     9781 aaccgttttt cagggcactt gatttgacta ataatattat ctttgtatcc gcagaaagag
     9841 cgggaaggat aaaaaaagcg attttctgaa agggtggttt tctgttttta acactaaatg
     9901 aaaacctatg aagatcaggg atatttttga gcataacgaa aaatggattg ctgagcgcct
     9961 gagggctgat ccggattatt ttgtgaaatt atcaggtcct cagaaaccgg aatatttgtt
    10021 catcggatgt tccgacagcc gcgtttccgt tgaatccctg ttgggcacaa agccgggaga
    10081 tgttttcatg caccggaata tcggaaatgt tattcccagc aatgatttaa atgccctttc
    10141 agtaataagc tatgcagtaa accatataag gataaagcat attatcgttt gcggacatta
    10201 taactgcggt ggtgttaagg cagcaatgac tgcccgtgat gaaggccttc tgaatccctg
    10261 gctcaggcat atcagggatg tgtaccggat ccacagaaat gaactgaaca gcattgatac
    10321 gcccggcaga cgctttgacc gcctggttga actgaacgtt gtggaacagt gtatcaatat
    10381 tgtcaagacc cgcgatgttc aaaaggccct gctggagaaa agaatatccc ttcacggctg
    10441 ggtgtttgat ctccgtacgg gcaggcttat tgacctaaag ataaacctgg aaaagattat
    10501 ggatgaaatt atggatgtct acaatttgtt tgaataattg ttgcccgcat ttcgcccgga
    10561 tcctcctggt tagtttccgt acctggcagt cctggaatcc tttataattc ctttccagtt
    10621 aagaccgaaa ccaaaatcat acacgtttcc ctcgctgtcg gtatggcatt tcatgtcacg
    10681 gggcacatcc tcattctgtt cctcaacggt tctcatgtct gccggcatct gaaaaggcag
    10741 caatccggag ggttcttcgg ctcctgttat aaggtccagc aaagcctgat cctgtacacc
    10801 aaaatgaatc aatatggatg atgcactttt ttctatttct gaaaatacca tggggtttga
    10861 cacatttata agcactataa ctggtttgtt tcccatctgt gcccttgttt cattaaccat
    10921 tttcatgtca taggaattat ttgccgttac tgtctttcct ttataggaac ggttcgtgaa
    10981 ggtttcaaag gggctgccgc ccgcaatgct tgctgcccgg gcatgaactg ccgtgtatct
    11041 tgaatactga agcggaatgg gaacatagcc gttgcctttt gccttcaggt cgtcggtgct
    11101 gtagcccgtg ccacctgacg gactggtgat tgaaaccagg gcaaaatcag cctctgaggg
    11161 gtcattgaca atcccgaaat agtttgttac aatgttcaga ttcaacgggt cagtccattt
    11221 ttccggtgtt gtctgtccga accagtttct tcctgccgga acatattttt gcggaatgaa
    11281 taccttcaga ttcttcttga gaggaagagt tttggctgta ttcttaagca taaccactga
    11341 tttcagctgg gcttcatatc ctgccttcat gaattccgga ctgccgacaa tgctttttgt
    11401 tttttgcgga tccaggtagg gattctcaaa cagacctgcc ctgaatatat ttctcagaag
    11461 ccgtacagcg gattcttcca aacgcttccg cataaattct tctccgtttt cttttactcc
    11521 aatattataa gcttctatta caggaccttt ggcattgttt ccgccaaact ggtcaagacc
    11581 agccataatt atcctgtaat ggcgttctgc ttctgacagg gtttcagcac cccagggttt
    11641 cccctcaaac ctgtcgatgc tgctcacgtt tcctgtcacg ccccagtcgg tgcagaccac
    11701 accatcatat ttatataccc ctctcaagag atcgttgacg atatatttgc tgtaggcgtt
    11761 tcctacattc tcaccgtata tggtatcctg gtcatatgat atggtatagt aaggcataac
    11821 ggcagatgcc attgcagtgg gaccttccag tttaaaaact ccttcagtga agggtatcag
    11881 gtgatccctg aggttgtttc cgggatagac ggcatacgat ccgtatccga aatgaccgtc
    11941 tcttcctccc tcttcaggac cgcccccggg ccagtgtttt accattgcat tgacactctg
    12001 ataaccccag ccgccggaaa tctcatcagg gcctgaggat gtctggaacc cgtcaacata
    12061 agcacgtgcc atatctgcgg caagtgcagg atcctccccc atagtgccct caaagcggct
    12121 ccatcttggt tcggatgcca gatcaatctg aggcgaaagg gcagttgcaa tcccaagcgc
    12181 cctgtattcg gcagaagcaa tttctccgaa tcttttcata acggacggat caaatgtagc
    12241 tgcaatgccc agcgttccgg gccacaatga tatatttccc ccggcaccgg cattgtattc
    12301 tgcatgcgga tcgcttccat gcctcgggtc ggaacttgta tttacaggaa tgccgaatgc
    12361 aatgccttcc acgaatgact gtgcattatt gttccagact gcagcagctt cgggactctg
    12421 aacagatgta atcagcacat gtcttacatt gtcgtctgac agaaagattt tttgctgatc
    12481 tgacaaatct cccgcacctg caccactttc ggcataaggc tttccaccgt aagttccgcc
    12541 tcctgcagga gcgcgtcctg ccgcaggtat tgactgatga ctgctgtaga gcatcagacc
    12601 cgcaatctgt tcaacagtca tttgtgaggc cagatcgcgt gcacggtcat caaaaggaag
    12661 cctccagtcc tcgtacacgt caagttttcc gtttctgttc agatccttga aggcaaaccg
    12721 gtctgctgct atgattttaa cgcccgaaga cggagaatag ccaagagtca aacctccctt
    12781 gttgtgaatg agagtatggg attccagttt ttcctcactc catttccggg cacagcctcc
    12841 tgcaagcaga atcaggagga ttgccgcaga agtaataatg aaagatttcc tgattgtatt
    12901 cattgcctgt tatttaattg atttgttttg caagtcacag gtttttagcc acatcaaata
    12961 tccaaatatt tattgcaaaa caaaaactgc tgtaaagaat atattttttt gaaggaatat
    13021 tactaattcg ggttgaataa ttaataatat ttgataattg aactggagaa agcaggtatt
    13081 ttaatacccg taaacagttt tttttaacaa aaccttttat ttttttaaaa ataatgacgg
    13141 atctttttaa aaatgtttta atttagattt tattataccg aatcaggaga aatatttaat
    13201 gtttgttgtt agtaaacctg gatcagcgga aaaaatgatg ctatataaaa gaacagatct
    13261 gccgtgtctc aggcctcctt gttcagatta ttaagcaggt cacaaaacgg gcagatttct
    13321 ttatttaacc ctaaaattaa cacattatga gaaaaaaact attgatgttt tttacaattt
    13381 tcctgatgat atgtgcttcc gcccttgctc aggaacgcac tatttcgggc atgattactt
    13441 cggcagagga cgggtccgca ttgccggggg tgaatgtaag agttcaggga agcagcatcg
    13501 gaaccatttc cgatgcacag ggacgataca gcatcagagt tccatcggga ttcaacacca
    13561 ttgagttttc atttatcgga atggaatccc gggtggtaac gatcggtatt tcagataata
    13621 ttgacattgc catggaatcc tcgtcacaat tgcttgatga ggtggttgtt acagccctgg
    13681 gtattgaaag gaaggcaaaa gcgattggtt ttgcccagca ggaactgaaa tctgatcagt
    13741 tgtctgctgc aagggaaacc aatctgacaa gtttccttac agccaaggtt gcaggggtga
    13801 gagtgtcaaa aaccgcttcc ggtacgggag gttcctcagc gatcaccatc aggggagcca
    13861 agtcgcttct cggaaacaat cagcctcttt ttgtcctgga cggtgtaccc ataacaaata
    13921 tcgggaatac atcgggggga acatgggaag gacttgacct gggtgatggt atgagcgacc
    13981 ttaatccgga agacattgaa tcgatgcagg tgctgaaggg acccaatgcg tctgcacttt
    14041 acggatcccg gggaagcaac ggcgtcattc ttataactac aaaatcggga aggcagaaga
    14101 aaggaatagg agtggagctc aactccaatt tttccctgga aacgcttaac ctctttccga
    14161 catatcagaa ttattatgcc acaggctatg aagatacaaa tctctacggc agcttcgtgg
    14221 aaatccccga gggaagcggg aaattctatg agaccatgga tacatggcat ggtgacagct
    14281 ggggacctcc catggacggc aggagggtta ttgtagatcc cttcatgttt cctgatgaga
    14341 aacctttttc gagaacattt acactgctgc cccagccggc aaacaacgta agggaattct
    14401 acaacaaggg aatcggactg tcaaacaccc ttgcaatttc agcctcagat gagaaaactt
    14461 cagcaaggct ttctgtggga catggctcga ataccgggat cattcccaac tcaaaatggc
    14521 agaagcagac cattgtcctg aacgtgaatt cccgtgttac agattatctt tctttcgacg
    14581 gaaaaataag ttatctgcgt gatgagggca ataacaggcc aatacttggt accatgtacc
    14641 agggcggcaa tgtctcacag gtgtttgctg taatgggaag atatgttccc atggatttcc
    14701 ttaaggaata ttatgagagg accaaatcac caggcacttt ccctgcagta cagtataatc
    14761 catactatat tgtcaatgag ttgaaaagca gggatgtaaa agacaggatt atcggccaga
    14821 tttccactac ccttaaaatt accgactggc tcagtcttat gggcagggcc ggaacggatt
    14881 attatacact gaaacttgtc aggacctggc cagtaggttc caagataagc gacaataccc
    14941 ggggacgggt ttataacgag ataagccttg taaaagacat taatgctgat attatactta
    15001 cagccacaaa agagctcagc agccagattt caggtaatct gtcggttggt tccagtattt
    15061 tacaccagga aaggaacaac cagtccattg acggaagagt ttttaaggca cccggagtat
    15121 atgatgtaag caactgcaca gatatccggc cctatacatg gctttcaagg aaacagatgc
    15181 agtctgtttt cttcaccggt cagcttgcat acaacaatta tcttttcctt gatgtgaccg
    15241 gcagaaatga ctggtcctcc gcactcggaa taaacaatta ttccttcttc tatccctctg
    15301 taagcacaag cttcgtcttt accgatgcat tcccgtccct tgcaggcaat gtcctgacat
    15361 tcggaaaagt aagggcatca tgggcacagg tgggaaatga ttcagatccc taccttacca
    15421 ggaacggata tgcgtcaaca accacaacct atgccgggca accgatttca tggatgaaca
    15481 gtacaattcc gctttttgac ctgaagaatg aactcaccgc atcgtgggaa atcggaacca
    15541 atctgaggtt cctaaataac aggattgatt ttgactttac ctattacgac ggtaaaacca
    15601 caaatcagat tcttccgata agcatttcaa attcaagcgg atacagtgat gtcgtgatca
    15661 acgccggaga gattcagaac aagggaatag agattgcact gaatctgaat ccggtcagaa
    15721 catcaaccgg tttttcatgg gatataaatg caaactattc caggaacaga tccatggttg
    15781 tttccctggc ccccggaatc gagacacttg tagtcggagg gggtgcttcc gccacgattg
    15841 aagccaggcc gggccagccc tacgggaaca ttgtaggata tccctatatg agagcccctg
    15901 acggacaaaa aatcgttgat gagaccggca gttacctgtt tgatccccaa ctcactgtcc
    15961 tgggaaatat cactcccgac tggattggag gcttaaacaa caccttttca ttcaagggtt
    16021 tctctatgaa tgtccttctt gattttgttc agggcaatga aattacatct gtaacaaaat
    16081 accagatgga agccaaagga accggtaaat ggacagttga gggaagaagg ctgagggata
    16141 tggatgatga gggaaatcag ctgcccctgg taggtattct tgacggagtt gttgaaatag
    16201 acgatggtga aggcaatatc acctatgaaa aaaataccaa ggctgttgat ggccagactt
    16261 actgggcaat gagggcctgg ggatccattt ctgaagaatt tgttatggac ggcagttata
    16321 tcagtctgcg tgaagtaatg ttgtcctatt ccttcagtcc gtccagactg gcaaaaactc
    16381 cctttgcagg aataacactc agcctcatag gacgtaatct gctgtacctt gaggaacaca
    16441 tgatgggaat gggagtttct cccgaatcct ctccaaacac ttcagccgga tgggcaggta
    16501 cggaatattt tgccatgccg acaactcgta catggggttt caatgttaag ctcacatttt
    16561 agtaaaaccc gcaaaaattg agaacaatga aaaaatcatt tataagtatt acgactttcc
    16621 tgcttgcact ggttttactg atatccaatg cctgcacgaa gaattatgaa gaaatgaata
    16681 cggacccgaa gattgtaacc gctgatatta ttgatccggg tttgttgctt acctatgtac
    16741 agtcgcaagc aattgtgata ggtggaagtt atggcaactc ggctgatggc agtttatccg
    16801 gaatgtgtac gaggggggat ggcagtccct ttggtgaggc ggatatgccg ggaatgtgga
    16861 atttttctta caccgttaca ttaaataacc tgtcggatat tatccatatt attgaaaaaa
    16921 gggaaaacag ggacgaattg gttaataaaa cggcgattgc caggataatg aaagcatggt
    16981 ctgtcagccg gctgactgat acctatggtg atgtgccgta ttcagagtca tgtcttcccc
    17041 aggaacaggc tatttacagt cctaaatatg acagccagga gagtatttac agggaccttt
    17101 ttaaggaact gaaggaagca gtcgcccaga tggatccttc caaacccagt ttcggaagtg
    17161 ccgacctgat ttacggaggt gatgtgggta aatgggaaaa actggccaat agtctgagac
    17221 tcagacttgc gctcaggatc agatatgcag atccggatct tgccagggcg cagctcagcg
    17281 atctgactga agcagacctt atgtctgacc tcggtgatga tgcctacatc ctgaacagta
    17341 cggaatatcc ggagcatctg aaccccaggt attttagaat tataaactgg ggaactgttt
    17401 cggaagaggt ccata
//
LOCUS       DAOSPX010000028        16777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3532_length_16777_cov_4.281964, whole genome shotgun sequence.
ACCESSION   DAOSPX010000028 DAOSPX010000000
VERSION     DAOSPX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16777
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3532_length_16777_cov_4.281964"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2014
                     /locus_tag="PLZ75_02625"
     CDS             <1..2014
                     /locus_tag="PLZ75_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS71325.1"
                     /translation="RLNARVTLDNDLTKWLKLSANIAYAKSKRDRMSSDLMRNTSEYF
                     AILPAYQYPNDPAIYGAFAGTWPTARDFNVGENRPGATFMLNQDEGYYKQDEFTGAFE
                     LTAQITPDLTFRTNLSVDNRNQTSRWFSGDYQGRRNSDANAQHWLWQYWQSENYFNYS
                     KTIADHQITGLVGLSWSENKYDYVTASASKFSSTFYQWNNLGAGSNPPTVGSSNTRGS
                     LNSYFARATYSYQGKYMLTATGRIDGSSKFGSNSKYGFFPSVGVAWRISQEGFMADIK
                     TISNLKLRISAGQTGNQEIGNFITQTYISNANVLLANGYNAGLYPGSTGNDNLKWETT
                     TQYDIGLDLGLIKGRISLTVDVYDKLTTDMLFNLPLPQSTTTGSAYVNYGSVKNTGIE
                     FSLNTVNFQRKNFSWETLFTIASNKNEIMELGPTGADIYTDTGAGNGTSVYRVGEPIG
                     TFFGLNRMGTWGTMEAVEAARYGRLPGDLKFEDVNNDGKIELLTDGDVIGHSYPKFYG
                     GLNNTFTYRNFDASINIMFVGGVDKAIVHESAEDRQFVNGMMNTVLDGWRPDHQNTMI
                     AQVRAGNAGARYDSYPDTHMIWNAAYIRGQAASLGYTIRGNLGGIDRLRVYATADNFF
                     LLTAVELPGYDPEGSAIQKINVRVPNIDKYQHPVPTTFSLGVNISF"
     gene            2033..3661
                     /locus_tag="PLZ75_02630"
     CDS             2033..3661
                     /locus_tag="PLZ75_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS71326.1"
                     /translation="MKYILKNNIALRFTILLVIFLMALSGCEDFLKEVPTNQLTTAAD
                     LTSLQYKEPFTVGPYRRLQDWTSGAGDWGNNIPSTIEYPTGGAYTDEPHVLFNKFATN
                     QVTGSLLDDYNNPWFYWYSGIQDCNLSIQQLPLIKMSETELNKALGEVRTLRAFYYFC
                     IVRYWGDAVLITKPITDVWATELPRTSLKTIYDEIIIPDLEFAVANLPAGRSTIGRVT
                     QDVARAILADVYMTVAGYPYQEVASNPSKDWCGTAAWAEQTYPVASGLEFLKKAQTQL
                     NALYNSVYTLGTYDDLHDPAMNNKGEAIFQVQYNATLRGNGVLQPSLPLLSAISPKEE
                     NGTFTPWVGYYNSYSDNDLRKKERQFFFTWDTKYSNVNDTVRFDVPFLYKQYVKAAVK
                     GTDGSGLNWTHYRYGEILLNLTEVNWALRQLGQSVSDDDIVKGINEIRKRALLPTYTA
                     DQVGLKEILAERAYELIFENKMLWDQRRTRKCVVYGNGEISSIQNFIGHQPAIFNFAF
                     APMHLLSPIPGNEIARNGLATQNSGYLPVQTKQN"
     gene            3693..4142
                     /locus_tag="PLZ75_02635"
     CDS             3693..4142
                     /locus_tag="PLZ75_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71327.1"
                     /translation="MKAKIIITLSFIAAIAIMTGCTKQPYYDIPYDENGNVYITKISI
                     ITSTGVTTADDSFTINCYFPNAKSGDVMKATVLQQQVPSWNPTGAKQLLPVAGSEKSI
                     TVGADLKTSVTYTRTEAHLLNVGDAVTVVFSGATDSGIISIVLKAAP"
     gene            complement(4754..6025)
                     /locus_tag="PLZ75_02640"
     CDS             complement(4754..6025)
                     /locus_tag="PLZ75_02640"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOS71328.1"
                     /translation="MKQSIIIVLAVTFLVLVSCNNVTVPGLHPGSPESAGVSAGRLAR
                     IDTVLKEYIDSGWIAGAVGFISRNGKIIYHKAFGVSDIENNTPMRTDNIFRIASQTKA
                     IVSVGMMMLYEEGKFLLDDPVSKYIPEFKNPAVLDKFSERDTSYTTLPARREITIRDL
                     FTHTSGIDYAGIGSDVMRKIYTKGGIRGGFGGDEVTIGDDMVRLGKMPLVHHPGEKFT
                     YGLNVDVIGYLIEILSGEKLDIWLKKRLFGPLGMNDTWFYLPPDRHGRLVKVSTENRE
                     GKAVSMPQNLADYPLLAGGKYFSGGAGLSSTTGDYAIFLQMLLNRGVYNGKRLLARRT
                     VELITSNQIGDLSIGRNKFGLGFEIMTEEGQAQLGITEGSFSWGGYYGTIYWADPKER
                     LVGQLYMQQVPISHSDIENKFKAMVYQALDD"
     gene            complement(6111..6920)
                     /locus_tag="PLZ75_02645"
     CDS             complement(6111..6920)
                     /locus_tag="PLZ75_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOS71329.1"
                     /translation="MKSTVKNKGGTLILFVLLSALFFPRNACSQPVVALDNWFNREIH
                     PRTGIPYHYLWTDTEFSGYSEWGRIFKARGAVITTIEKPLRSVLNDADVYIIVDPDTT
                     TESASPNYILPEDIRVIKRWVRKGGVLAIFANDGPNCEFTHLNKLASKFGIRFNHVSL
                     YPVTNNDWEMGAITNLPDHPLFRGVSKIYLKEISDMKLKRQARPVLTAGGRVLMAEND
                     FGKGYVFAVGDPWLYNEYIDHALLPESFENLKAAKNLTDLLLEKTGKKARE"
     gene            7256..7666
                     /locus_tag="PLZ75_02650"
     CDS             7256..7666
                     /locus_tag="PLZ75_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71330.1"
                     /translation="MRRTICLSFIAIALLLTACSKEKESERFRLLTTPVWVSESILVN
                     GVKPEGDWDFLNDFTGDAKFHKDGTGYFGSFTGEWKFNDAETEIIITTTTVPIPIVTE
                     IIELTSVTLKISTTILNPQNPAESADVIMIFKAR"
     gene            7663..9897
                     /locus_tag="PLZ75_02655"
     CDS             7663..9897
                     /locus_tag="PLZ75_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS71331.1"
                     /translation="MIRHFLTVILCILCCCSLASGAEGNIKGRVIDSEHGEPLAGVYV
                     IYGSNRGTTTNDSGYYFIADVSGRVRVVFQFVGYATVTRDVTVRADETVELDISLETL
                     VSEIDQVVVSANRTEQKVAELSVSMSVIKNSFLSDNHISDARELIGKNPGIDVMDGQA
                     SVRGGSGFSYGAGSRVLALIDGLPFNSADAGNIKWQFLPLENLSRIEVIKGASSVLYG
                     SSALNGIINFRSADASNTPVTKFHTEAGIFGRPRNREWKWWNTPRFFTNTSLSHLQKF
                     GKTDIGMSAYFMTDEGYRRLNGENLGRISLRLKHFSTKVEGLNYGLNISAGSTHKTDF
                     VLWENARTGALRQSEATAAEFRGNFITIDPFISLRKNERTRHDLKIRFRSDRNRLPRN
                     EKNNSDAVNLYSEYQLWCRITDFLDITSGLTENYSLVNSEFFGDHHGLNLAGFSQVEI
                     RPVNRLKAVAGLRLEHFSLDGVSDRLTPILRAGINFQAAEYTYLRASFGQGYRYPSIA
                     EKYASTTLGSVKIIPNPGILAEKGWSTELGVKQAFLLGKTAGQADLSFFLMQNSNLIE
                     FLFASYHGEGVGFRADNLEQARIYGSEFEFAFETRLNRVNISSTGGYAYTFPRDISNR
                     YSDKIVFLKYRRKHSVKLYVNSSWKSFTLGSGLNIRSKILNIDNVFLEPLTRELFLPG
                     FYEYWNRNNRGHAVLDVSAGYIISPRLNLSFVVKNFTNTEYMGRPGDIQPHRNFSIRL
                     SGNL"
     gene            10020..10646
                     /locus_tag="PLZ75_02660"
     CDS             10020..10646
                     /locus_tag="PLZ75_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOS71332.1"
                     /translation="MCHQLQMNMPSTVLNNNRSFSSGMLLFLSAEMLFFGLLFIVYLA
                     YRISYAQAFHFASGGLSLARGTVSTVILLISSATLAKSFDAIRQDNKRLAQQLTGITI
                     LLGILFLLTGCFEWQQHIRAGIYPASSVLALRGPGDVLFYGLYFFITGLHALHVIIGL
                     AVIGFLMRGIIRGKTDSGNYDSARRKGLYWYLVVIIWVFIFPLFYLIT"
     gene            complement(10667..11845)
                     /locus_tag="PLZ75_02665"
     CDS             complement(10667..11845)
                     /locus_tag="PLZ75_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71333.1"
                     /translation="MKEQLQVSTRFKTVCLILAAAGAGTFAAGLFSDRETAWAAYLVT
                     NYYFFSLSMGGVFFYVIQNITQSGWSSAFKRVPEAMMAYMPFAAVFFLLLYFGTSHIY
                     RWSLPEVIAGDELLQNRTAYLNVPFFYLRIFICFALWVIFIRILRILSLREDSLDPAD
                     QDGIMALFSKSELYSKIFIFILIITFSLSAFDLIMSIDVHWYSTIFAFKNLIAAFLHG
                     VSVMVLIIFILHKRGYFSFLNQYHLHDFARYIFMLSIMWGYFWFAQFMLIWYGNIPEE
                     TVYYYLRWQKGWETLFFLEIGLNWAVPFMLLLPVKTSRNMTVITVVIIFLIIGQYVDL
                     FVQVIPGTTGMLRFGLTEAGLFLGYAGLFALVVATALSKARIIGPNHPYLEESMKHKF
                     "
     gene            complement(11890..12477)
                     /locus_tag="PLZ75_02670"
     CDS             complement(11890..12477)
                     /locus_tag="PLZ75_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOS71334.1"
                     /translation="MKIYGTAFLVLFSLVQFSCNRDRNHPGWDYFPDMFYSTAYETFS
                     ENPNFTDGMTMREPVPGTVPRDFVPFGYTADAESRARAGNELANPFSPAAAVLDRGRE
                     VYTIFCAGCHGIRGNGDGELFTAGLYSLQPKSLTDSIALSLKDGEIFHTITLGLGFMG
                     AHGSQVLPDDRWKMILYIRELQDRLPGPAGSGPQR"
     gene            complement(12495..13028)
                     /locus_tag="PLZ75_02675"
     CDS             complement(12495..13028)
                     /locus_tag="PLZ75_02675"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HOS71335.1"
                     /translation="MARTFKIGIFDDEEKFISSVRTLQEQQYPIHEVYTPYPVNEVFH
                     LLKRNTRLPTAAYFFGLAGIIATLAFLYYTSVISWPIVYGGKPFNSFPSFIVVTIVLT
                     ILVVTILSLAVFSGRSKLYPGRENTVFDSRATDNKFIIVMDTEILNQPMSGRAADIMK
                     AEGAIEIIDKEIENVNT"
     gene            complement(13040..14401)
                     /gene="nrfD"
                     /locus_tag="PLZ75_02680"
     CDS             complement(13040..14401)
                     /gene="nrfD"
                     /locus_tag="PLZ75_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOS71336.1"
                     /translation="MYNRKIRGPLIEGNKTFGQITKEVVAPLETRPGLWWYIALAVSS
                     AAAVYGVYAMYITVTRGIGTWGLNNSVAWGWDIINFIWWIGIGHAGTAFSIFLLVLRQ
                     RWRTSINRSAEAMTVLAVACAALFPLIHMGRIWLAFYIIPYPNTRGPLWVNFNSPLFW
                     DFTAITAYLLISVSFWYLGMLPDFGTIRDRAGSRIRKAVYGFFSLGWTGSSRQWIRFE
                     SLSFVFGGLAAVLVVSVHSIVATDFAAGIVPGWHSTIFPPFFVVGAIFSGFAMVMTLM
                     VIIRKIYKLTDYITDNHMDAIARILIFISLIMAVSYLIELFIAWYSGNEYEMHAIIGN
                     RVTGDYALQFWGMIVCNALIPQLFWFRKIRRNWLALLLISLVINLGMWLERYNIVVTP
                     LSKDFLPSSWVTYTPTVIDIGVFAGTIGLFAIGVLLFMRYIPMMAISELKGVLHIGKK
                     DED"
     gene            complement(14419..>16777)
                     /locus_tag="PLZ75_02685"
     CDS             complement(14419..>16777)
                     /locus_tag="PLZ75_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOS71337.1"
                     /translation="LTLQLLLVWHKYSAVLEANSLCFGKRVIPDYNFDRTDLVLSVNA
                     DFLGTWVAPVHFIPKYIARRKLNNGERSMLRHIHYEAGMSITGSNADLRRKLRPSEEK
                     IMLTDLYNRIAVNSGAETLPGPAFREDLSGLAESLMACRGRSIVVSGTNKVEIQIIVN
                     GINHLLGNYPDCIDPGRHLAIAAGIDSKTEALVNDMNDGKIRALLMYNVNPVYDYPEA
                     DKFLNGLSRTELTINMAVALNETAGRAKYECPVHHYLESWNDAEIIPGQVSLCQPAIH
                     PLFSTRSFQESLLKWSGNPVTWHDYMRSYWEREYFPGSGQSSFRNFWNTSLGKGVHTC
                     PVKAENMPPFDRTAFRKVSESRDLPSPDNYEVIIYESIGMGTGIYANNPWLMELPDPL
                     TRHCWENVACLSPSDAAGLGVVNGHLLKIGDGLLLPAFIQPGQAEGTISVAAGYGHTG
                     SGPVADGKGVNLYPFAKIKGGSRSYGFTVSRVENTLRESPLALTQTHSSMEGRPIVKE
                     TFLSRYRENPASGNNLHGASDLHDKTLYPEVKYDGFHWGIAIDLNACVGCNACVIACQ
                     AENNIPVVGKDEVRLRRIMHWIRIDRYYSESSDNPRVYFQPLMCQHCDNAPCENVCPV
                     SATNHSSEGLNQMTYNRCVGTKYCLNNCPYKVRRFNWYRYTDNRSFDFNTASDLGKMV
                     LNPDVTVRERGVVEKCSFCVQRIQEKKLRAKLENRTLNDGEIKTACMQACPSGAIVFG
                     NLNDKNSRVSRLFSDPRQYFLLEELHTLPSVGFLTKVWNDEDIKS"
BASE COUNT         4685 a         3853 c         3754 g         4485 t
ORIGIN      
        1 ccggttgaat gccagagtaa ctctggacaa tgatcttact aaatggctca aattgagcgc
       61 caacatagca tatgcaaaaa gtaaaagaga ccgaatgagt agtgatttga tgagaaatac
      121 ctcggaatat tttgctattt tgccagcata tcagtatcct aatgatcctg caatatatgg
      181 agcttttgca ggaacatggc ctactgccag ggactttaat gttggtgaga accggccagg
      241 tgcaactttt atgctgaatc aagacgaagg ctattacaaa caagacgaat ttaccggagc
      301 atttgaacta actgcacaaa ttacaccaga cttgactttt agaacaaacc tctctgttga
      361 taacaggaat caaacgagta gatggtttag tggagattac cagggcagac gtaactcaga
      421 tgcaaatgct caacattggt tatggcaata ttggcaatcc gaaaactatt tcaactattc
      481 gaaaacaata gctgatcatc aaataaccgg tttggttggg ctttcatggt ctgaaaataa
      541 atatgactat gttactgcat cggcatccaa attctcttct actttttacc aatggaacaa
      601 tttaggtgca ggtagcaatc cacctacagt aggctccagc aataccaggg gttcgttaaa
      661 ctcttatttt gcccgtgcta cttatagcta ccagggcaaa tatatgttaa cagcaaccgg
      721 gcgtattgac ggttcatcaa aattcgggag caactctaaa tatggtttct ttccatcggt
      781 tggtgttgca tggagaatct ctcaagaggg ttttatggcc gacatcaaaa caatatctaa
      841 ccttaaactg cgtataagtg ccggacaaac cggtaaccag gaaattggta attttataac
      901 acaaacgtat attagtaacg ccaatgtttt attggcaaat ggctataatg caggccttta
      961 tccaggctct acaggtaacg ataacctgaa atgggaaaca acaacacaat acgatattgg
     1021 tttagatctt ggcctgataa aaggtaggat cagcttaact gtggatgtgt atgataaatt
     1081 gacgaccgac atgttgttta atcttccatt acctcaatca actacaacag gtagtgctta
     1141 cgtaaactat ggttcggtta aaaacaccgg catcgagttc tcattgaaca cggtcaattt
     1201 ccaaaggaaa aacttttcat gggaaactct ctttacaata gcatccaata aaaatgagat
     1261 tatggaactc ggccctactg gtgccgacat ttacacggat accggagcag gtaacggaac
     1321 cagcgtttat agagttggag aaccaatcgg gacattcttt ggattgaacc gcatgggaac
     1381 ctggggcact atggaagcag tggaagcagc gcgttatggc agactaccgg gagatttgaa
     1441 atttgaagat gtgaacaatg atggaaaaat tgagctgctg accgacggcg atgttattgg
     1501 tcactcatat ccaaaattct atggtggcct taacaacaca tttacttata gaaactttga
     1561 tgcaagtatt aacattatgt ttgtaggcgg agttgacaaa gctattgtgc atgagtctgc
     1621 agaagaccgc cagtttgtaa atggtatgat gaatacagtt ttggatggat ggcgtcctga
     1681 tcatcagaat acaatgattg ctcaggttcg tgccggtaat gccggtgcac gctatgactc
     1741 ataccctgac acccatatga tttggaacgc tgcatacatt agggggcagg cggcttcact
     1801 aggttatact atccgtggaa accttggtgg tattgataga ttgagagttt atgccactgc
     1861 agataatttc tttttgttga ccgcagttga acttccaggt tatgatcctg aaggtagtgc
     1921 tatacagaaa attaatgtca gggtaccaaa tattgataaa tatcagcatc cggttcctac
     1981 aaccttcagc ctgggtgtta atatcagttt ctaattttaa aattttcata ttatgaaata
     2041 catattaaaa aacaacatag cattaagatt cactatactt ttggtgatat tcctgatggc
     2101 tcttagtggt tgcgaggact ttctgaaaga agtgcctaca aatcaactta ctactgcagc
     2161 agatctaacc agtttacaat ataaagaacc ttttacagtt ggaccttacc gacgtttgca
     2221 ggactggact agtggagcag gagactgggg aaataatatt ccatctacaa ttgagtatcc
     2281 aactggcggt gcatacacag atgagccgca cgttctgttc aataaatttg cgaccaatca
     2341 ggtaacaggc agtttactcg acgattacaa taatccctgg ttttattggt attcaggtat
     2401 acaagactgt aatctctcta tccaacaact tcctttgatt aagatgagtg agacagaact
     2461 taataaagct ttgggagaag tacgtacctt aagggcattc tattattttt gtattgttcg
     2521 ctactggggt gatgctgttc ttataacaaa accaataaca gatgtgtggg caacagagtt
     2581 gcctcgtacc agtctgaaaa caatttatga tgaaataatt attccagacc tggaattcgc
     2641 agttgccaat cttccggcag gcagatccac gattggtcgc gtaacacagg atgtagcccg
     2701 ggcaatactg gcagatgtgt atatgaccgt tgccggttat ccttatcagg aagtagccag
     2761 caatccgtct aaagactggt gcggtacagc agcttgggca gagcagacct atccggttgc
     2821 aagtggtctt gaattcttaa aaaaggcaca aacacaactt aacgcgttgt ataatagtgt
     2881 atatacattg gggacctatg atgatttgca cgatccggct atgaacaata agggggaagc
     2941 aatatttcag gtgcaatata acgcgacact caggggtaat ggtgttttac aaccatctct
     3001 tcctttgcta agtgcgattt ccccaaaaga agaaaacggc acatttactc catgggtagg
     3061 atattacaat tcctattctg ataatgactt gcggaaaaag gaacgtcagt ttttcttcac
     3121 atgggatact aaatattcca atgtaaatga tactgttcgt tttgatgttc catttctcta
     3181 taagcaatac gttaaagcgg ctgttaaagg gacagatggt tccggtctga actggacgca
     3241 ttaccgttat ggagaaatac tgttgaatct tactgaagta aactgggcgt taagacaatt
     3301 aggccagtcg gtatctgacg acgacatcgt aaaaggaatt aacgaaatca ggaaaagggc
     3361 attacttcct acttatacag cagatcaagt tggtctgaaa gaaatcttag ctgaaagagc
     3421 atatgagctg atattcgaaa ataaaatgct ctgggatcaa cgtcgcacaa gaaaatgcgt
     3481 tgtatatggc aatggcgaaa tttcatctat tcagaatttc attggtcatc agcctgcaat
     3541 tttcaacttt gcttttgctc caatgcattt attatcacct attcctggta atgaaattgc
     3601 cagaaacggg ttagcgacac agaatagtgg gtatttgcct gtacaaacta aacaaaatta
     3661 agtattgcat ttttattatt taactaaaaa atatgaaagc gaaaattatt ataacacttt
     3721 cattcattgc tgcaatagcg ataatgacag gttgtacgaa acaaccttat tacgacatcc
     3781 cttatgatga gaacggtaat gtttatatta ccaaaatatc aataataact tctaccggag
     3841 ttacaactgc tgatgattca tttacaatta actgctattt tccaaatgca aaatccggag
     3901 atgttatgaa ggctactgtt ttacaacagc aggtacccag ttggaatcca actggtgcca
     3961 aacaacttct gccggtagcc ggtagcgaaa aaagtattac ggttggggct gatcttaaaa
     4021 catcagtaac ttataccaga acggaagcac atttgttaaa tgttggtgat gctgtaaccg
     4081 ttgtattttc cggagcaaca gattcaggca taatatcaat tgtgcttaaa gcagccccat
     4141 aatgtacttt ctttttcaca aatagttttt aaagaggctg tctttagggg cagtctcttt
     4201 aaactttaaa tcgaatgaat atccgccatt atacctaaga agaacctgag ccggcctgga
     4261 caggcagaat atatcagacc atatttgttg aaagatctta agattgtcag gcctaatcag
     4321 gaataggcta ttaatataag tgcgccagga aacgatcatc tcaataatga gaaatcgtcg
     4381 ccgttttttg aaccggcagt tgaaatactg caccattata caagggaagt atggtggaag
     4441 ggggttgtga agtttcatta tgtgctgagt atgaaacgaa ataagcagaa caaccttaaa
     4501 tgtaccggag gcaagccatt tactgccggt cgggctacca tagaatctat accaagagtt
     4561 atggaactgg gtaacagata tccgtaatgg aataaacgcg gcaatagagt cacgcagtcg
     4621 tgaccgacga tctggaagaa cagttttcct gttttctccg ggctgtatta ccgtacacaa
     4681 tctccgggca gcatcctcgt gaatctgcaa accacggcct gtataaattc tgaggaaata
     4741 cggcagctgt gattcagtcg tccagtgcct ggtagaccat tgccttgaat ttattttcaa
     4801 tatcactgtg gctgatcgga acctgctgca tgtacagctg accgacgagc ctttccttcg
     4861 gatcagccca gtaaatggtt ccgtaataac ctccccatga aaaggagcct tcagttatac
     4921 caagctgtgc ctgaccttct tccgtcatta tttcaaaacc cagtccgaac ttgttacggc
     4981 ctatgctcag atcacctatc tggtttgagg taataagttc aacggttctc cttgccagga
     5041 gccttttgcc attgtatacg cccctgttca gcagcatctg gaggaatatg gcatagtcgc
     5101 ccgtggtgga actcagacct gcccctcccg aaaaatactt tcctcctgca agcagcggat
     5161 aatcagcaag gttctgaggc attgacactg ccttcccttc cctgttttca gtgctcacct
     5221 tcaccagtct gccatgcctg tcagggggca ggtaaaacca ggtgtcattc attcccaggg
     5281 gcccgaaaag ccgtttcttc agccagatat caagtttttc acccgacagt atttcgatca
     5341 gataacctat cacatccacg ttcaggccat aggtgaattt ttctccggga tgatggacaa
     5401 gcggcatttt tcccagacgt accatatcat cccctatagt gacttcatcg cctccgaatc
     5461 cgcctcttat tccccccttt gtatatatct tcctcatcac atccgaaccg atgccggcat
     5521 aatcaatgcc cgaggtatgc gtgaaaagat ctcttattgt aatctcccgt cttgcaggca
     5581 gggtggtata gcttgtatcc ctttcactga acttgtccag taccgccggg tttttgaatt
     5641 cgggaatata ttttgagacc ggatcatcaa gcaggaattt tccctcctcg tacaacatca
     5701 tcataccgac actgactatg gcttttgtct gggaagctat cctgaaaata ttatctgtcc
     5761 gcatgggagt attgttttca atatcgctta ctccaaaggc tttgtgatag attattttgc
     5821 cgttgcggga aataaatccg actgcgcctg ctatccaccc gctgtcaata tattccttca
     5881 gcacagtgtc aatacgggcc aggcggccgg cagaaacccc tgccgattcc ggggaacccg
     5941 gatgaaggcc gggaacagtg acattattgc aggaaacaag gacaagaaaa gtgactgcaa
     6001 gtacaataat aatcgattgt ttcattttat gatattttaa aaaatgtgat taacctgtat
     6061 tgttgccgga cagctgactg acttcacctc agtccatcca agcactcttt ttattcccgt
     6121 gcctttttac ccgtcttctc cagcaggaga tccgtaagat tctttgccgc tttcaggttc
     6181 tcaaagcttt ccggaagaag ggcatggtca atatattcat tgtagagcca gggatctccg
     6241 acggcaaata catacccctt tccgaaatcg ttttccgcca tcagcactct tccgccagct
     6301 gtcaggaccg gtctggcctg ccttttaagc ttcatatccg aaatttcctt gagatagatc
     6361 tttgacactc ccctgaaaag cggatgatcg ggcaggttgg ttattgctcc catttcccag
     6421 tcgttgttag tgacaggata gagtgaaaca tgattgaatc tgattccgaa ttttgacgca
     6481 agtttattga gatgcgtgaa ttcacagttc ggcccgtcat tggcaaagat ggcaagaact
     6541 ccccccttcc tgacccacct ctttatcaca cgtatatcct cgggaagtat gtaattcggt
     6601 gatgctgact cggttgtcgt atcaggatca acaattatat agacatctgc atcgttaagt
     6661 actgaacgga gcggtttctc aatggttgtt atcacggcgc cgcgggcttt aaatattctg
     6721 ccccattcgg aatatccgct gaattctgta tcggtccaca gataatgata gggaatgccg
     6781 gttctgggat gtatttcccg gttgaaccag ttatccaggg caactaccgg ctggctgcag
     6841 gcattccggg gaaaaaacaa tgctgataac agtacaaaca ggatcaaagt cccgccctta
     6901 tttttcactg tgcttttcat atttcttatt tttcatataa catcctggca acctgcattg
     6961 tcaaataaaa aacagcaaca tcatgcaaca ttcataccgg tcagtgtttt cagagccgtt
     7021 gaattttatg cttcttgctg attgttaatt caaaaataga aaagaaatgt gatgattgaa
     7081 atttccacag ggatatattt tcgccctgcc ttatccgcga ttgtaaattc ccggtaacag
     7141 gaaatgacaa tactgccatg aaattcgtaa ttttgcaatc agagacaggg ccatgatact
     7201 ttgttggaga cctttcatgt aaacctggca tgattgtatt aaaaattgaa tcataatgag
     7261 aagaactatt tgcctatctt tcatcgcaat tgccctgctg ctgacggcat gcagcaagga
     7321 aaaggaatct gaacgcttca ggcttctgac gacacccgtg tgggtcagcg aatccattct
     7381 tgtaaacggt gtaaagcctg agggagactg ggactttctg aatgacttca ccggagatgc
     7441 aaaattccat aaggacggaa caggatattt cggaagtttt accggagagt ggaaattcaa
     7501 tgatgctgaa acggaaatca tcataaccac aacaacagtg cccattccga tagtaacgga
     7561 aataattgaa ctgacttccg tgaccctgaa aatatccact accattctca atccgcagaa
     7621 tcctgcagaa tcagctgatg taatcatgat cttcaaagca agatgatacg ccatttcctc
     7681 actgtcattc tctgtattct gtgttgctgt tcactggctt caggtgcgga aggcaacata
     7741 aagggaagag tgattgacag cgaacatggt gaacctcttg caggtgttta tgttatttac
     7801 ggaagcaacc gcggaacaac caccaatgat tcaggctatt acttcattgc agatgtttcg
     7861 ggcagggtca gggtggtatt ccagtttgtg ggctatgcca cggttacaag ggatgtcacg
     7921 gtgagagcgg atgagacggt ggaacttgac atcagccttg aaacactggt cagtgagatt
     7981 gaccaggttg ttgtgagcgc taacagaact gagcagaaag ttgcagagct cagtgtttca
     8041 atgtctgtta taaaaaactc ctttctttct gacaaccata taagcgacgc aagggaactg
     8101 ataggcaaga atcccggaat tgacgtgatg gacggccagg cgtcggtaag gggaggaagc
     8161 ggattcagtt acggggcggg aagccgtgtt cttgcactta tcgacggact gcccttcaat
     8221 tccgctgacg ccggaaacat taaatggcag ttcctgccgc tcgaaaacct ttcccgtatc
     8281 gaggtgatca agggagcatc ctctgtatta tacggttctt cagctcttaa cggaatcata
     8341 aatttcagaa gtgctgacgc cagcaatacc cctgtgacaa aattccatac cgaggcggga
     8401 atatttggca ggccccgtaa ccgcgaatgg aaatggtgga atactcccag gttctttaca
     8461 aacacctccc tttcacacct tcagaagttc ggaaaaaccg acataggcat gagtgcgtat
     8521 ttcatgacgg acgaaggata caggaggctg aacggggaga acctgggcag gataagtctc
     8581 aggctgaaac acttcagcac aaaggtggaa ggacttaatt acgggttgaa cataagtgcg
     8641 ggaagcaccc acaagacaga tttcgtatta tgggagaatg cccggacagg ggccctcagg
     8701 caatcggaag caaccgctgc ggagttccgg ggaaatttca ttacaatcga ccccttcatt
     8761 tccctgagaa agaatgaaag gacaagacat gacctgaaaa tcaggttccg ttccgacagg
     8821 aacaggcttc ccaggaatga aaagaacaac agtgatgccg tgaatcttta ttcagagtat
     8881 cagctgtggt gcagaataac agatttcctt gatataactt ccggcctgac agaaaattac
     8941 agtctggtaa attcagagtt tttcggggat catcacggac ttaatcttgc cggtttttca
     9001 caggttgaaa taaggcccgt aaaccgtctc aaggctgttg ccggactgag gcttgagcat
     9061 ttctcacttg acggggtcag tgacaggctt actcccatcc tgagggcggg tatcaatttc
     9121 caagccgcgg aatacacata cctgagagcc tcattcggcc agggctaccg ctacccttcc
     9181 attgccgaga aatatgcatc aaccaccctg gggtccgtga agataattcc aaatccggga
     9241 atcctggctg aaaagggatg gagtaccgaa ctgggggtca agcaggcgtt tctactggga
     9301 aaaactgcgg gccaggccga cctgtcattc tttctcatgc agaattcaaa cctgatcgaa
     9361 ttcctgtttg ccagctatca cggtgaaggt gtcggtttca gggcagacaa cctggaacag
     9421 gccaggatct acggcagtga gtttgaattt gcttttgaaa cacgccttaa cagggtgaat
     9481 atctcatcca ccggaggcta tgcctacacc tttccgaggg atatcagcaa caggtacagc
     9541 gataagatag tcttcctgaa atacagaagg aagcattcag tcaagctgta tgtaaactca
     9601 agctggaagt ccttcaccct gggttcgggt ctgaatatac gctcaaaaat tctgaacatt
     9661 gacaatgtgt ttctcgagcc cctcacacgt gaactctttc tgccgggctt ttatgaatac
     9721 tggaacagga ataacagggg ccatgccgta cttgatgtct ctgccggata tatcatcagt
     9781 ccgcggctca acctgtcatt tgtggttaaa aactttacaa acaccgaata tatgggcagg
     9841 cctggcgaca ttcagccaca caggaatttc agtatccgcc tgtcagggaa tttataataa
     9901 tacacggacc tgttcatgtt tttattcatt tgggattttt cagctttgga tttggaaatt
     9961 aatagtttta tctttaaacg atgatccggc ctgcctcatg caggatcacg cgacgctaaa
    10021 tgtgccatca actccagatg aatatgcctt caacagtgct taacaacaat cggtcattca
    10081 gttccgggat gctgcttttc ctttctgccg agatgctctt tttcggactg ttgtttatcg
    10141 tttacctggc ataccgtatc agctatgccc aggcatttca ttttgcctcc ggcggattat
    10201 ccctggcaag aggtacggtg agcacagtca tcctgctgat cagcagtgcc acccttgcaa
    10261 aatcgtttga tgcaatccgt caggacaaca aaaggcttgc acagcagctt accggcataa
    10321 ccatcctgct cggaatcctg ttcctgctta caggctgttt tgaatggcag cagcatataa
    10381 gggccgggat ctatcctgcc tcttccgtgc tcgctctccg cggcccgggg gatgttcttt
    10441 tttatggcct gtactttttc ataacaggac tgcatgcact gcatgttatt attggccttg
    10501 cagtgattgg attccttatg cgcggcataa tcaggggaaa aacagattca gggaattatg
    10561 actccgcccg caggaagggt ttatactggt atctggttgt cattatatgg gtctttatct
    10621 ttcccctctt ctacctgata acataacaac ctgaggttct gagaaatcag aacttatgct
    10681 tcatgctctc ttccagatac ggatgattgg gtcctatgat ccgggccttg ctgagggccg
    10741 ttgccaccac cagggcaaac aggccggcat agccgaggaa cagtcccgct tcagtcagtc
    10801 cgaacctgag cattccggtt gttcccggga tgacctgtac gaaaaggtca acatattgtc
    10861 cgataataag aaatattatt accacggtaa taactgtcat gttcctgctg gttttgacgg
    10921 gaagcaggag catgaagggg actgcccagt tcagaccgat ctccaggaaa aataatgttt
    10981 cccagccctt ctgccagcgc aggtaatagt aaactgtttc ttcagggata ttgccatacc
    11041 agatcagcat gaactgtgca aaccagaaat atccccacat gatggagagc atgaatatgt
    11101 agcgggcaaa atcgtgcaga tgatactggt tgaggaaact gaaatatccc cgtttatgca
    11161 ggatgaatat gatgagaacc ataaccgaca caccatgaag gaatgcggca atcaggtttt
    11221 taaatgcaaa aatcgtactg taccagtgca catcaatgga cattatcagg tcgaaggcag
    11281 aaagtgaaaa ggtgattatc agtatgaaaa taaatatttt tgaatacagc tcacttttac
    11341 tgaaaagggc cataatgcca tcctggtcag ccggatcaag gctgtcttcc ctgagcgaca
    11401 gaattcttaa tatacgtatg aaaatgaccc acagggcaaa gcatataaat atcctgaggt
    11461 agaaaaacgg gacattcagg tatgccgttc tgttctgaag cagttcgtca ccggcaataa
    11521 cttccggcag tgaccaccgg taaatgtggc tggttccaaa ataaagaagc aggaaaaaaa
    11581 ctgccgcaaa aggcatgtat gccatcatgg cttcgggaac acgcttaaac gcggaggacc
    11641 atcccgactg ggtaatattc tgaatgacat aaaagaaaac ccctcccatc gaaagcgaaa
    11701 agaaataata attggtgaca agataggcag cccaggcagt ttcccggtct gaaaacaatc
    11761 cggcagcaaa ggtgcctgca cctgcagctg ccaggatcag gcatactgtt ttgaatcttg
    11821 ttgatacctg tagttgctcc ttcattattt ttactgtatt atgcaatgat attaggcaaa
    11881 ttctggttcc tatctctgag gtccgctgcc ggccggcccc ggaagcctgt cctgaagctc
    11941 ccttatataa aggatcatct tccaccggtc gtcaggcagt acctgtgatc cgtgtgcacc
    12001 cataaaaccc aggcccagtg tgattgtgtg aaagatctca ccgtccttca ggctgagcgc
    12061 aatactgtca gtcagggatt ttggctgaag cgaataaagg ccggcagtaa aaagctctcc
    12121 gtccccgttt ccacggatac cgtgacatcc tgcacagaat atggtataaa cctcccttcc
    12181 cctgtccagc acagcggcag ccggcgaaaa cggatttgcc agttcgtttc ctgctcttgc
    12241 cctcgattcc gcatcggcag tatatccgaa aggaacaaaa tcacgcggta ccgtacccgg
    12301 caccggttcc ctcatagtca ttccgtcagt gaagttcgga ttctccgaaa atgtttcata
    12361 ggccgtggaa taaaacatat cagggaaata atcccatccg ggatgattcc tgtctctgtt
    12421 gcaggagaac tgaaccaatg aaaacagtac aagaaaggct gttccgtaaa ttttcattct
    12481 caggctatta tacatcatgt atttacgttt tcaatttctt tgtcaataat ctcaattgca
    12541 ccctccgcct tcattatgtc tgcggccctg cctgacatag gctggttcag gatttcagta
    12601 tccatgacaa ttataaactt attgtcggta gccctgctgt caaataccgt gttctcacgg
    12661 ccggggtaaa gttttgatct tccggaaaac acggcaaggc ttaaaatagt tactaccagt
    12721 atggttaata caatggtaac cacgatgaag gagggaaagg agttgaatgg cttgcccccg
    12781 tatacaatgg gccagctgat tacagaggtg taatagagaa aagccagggt ggcaataata
    12841 ccagccagac caaagaaata agctgcagtc ggcagccttg tattccgctt taacagatga
    12901 aacacttcat ttacaggata aggagtataa acctcatgga taggatactg ctgctcctgc
    12961 agtgtcctga ccgaggaaat gaacttctcc tcatcatcga atatccctat tttaaaagtt
    13021 ctggccataa cttagaaaat taatcctcgt ccttctttcc tatatgtaaa acacctttca
    13081 gttcactgat tgccatcatg ggaatatacc tcatgaacag caatacccct atggcaaaca
    13141 ggcctattgt cccggcaaac acgcctatat caattacagt gggcgtatat gtaacccagc
    13201 tggaaggcag aaaatcctta ctcagcggag taaccactat attgtatctc tccagccaca
    13261 tcccgaggtt aatgaccagg gagatcagca gaagggccag ccagtttctt ctgatcttcc
    13321 tgaaccagaa gagctggggt ataagagcgt tgcagactat catcccccag aactgaaggg
    13381 cgtaatcacc cgtgaccctg tttcctatga ttgcatgcat ctcatattca ttgcctgaat
    13441 accatgcaat aaaaagctca ataagatagg atacagccat gatcagagag atgaatatga
    13501 gtattctggc tatggcatcc atatgattat cagtaatata atcagtgagc ttgtaaatct
    13561 ttctgatgat taccataagt gtcatcacca tggcaaaacc tgaaaagatt gctcccacaa
    13621 caaaaaacgg cgggaaaatc gttgaatgcc agcccggtac tattcctgca gcgaagtccg
    13681 tggcaacaat tgaatgaaca gacaccacca gaacggcagc aaggcctccg aaaacaaaac
    13741 tgagtgattc gaaacgtatc cactgacggc tggatcctgt ccacccgaga ctgaaaaagc
    13801 cgtaaacagc ttttctgatt ctagagcctg ccctgtcgcg gattgttccg aaatcgggca
    13861 gcattcccag gtaccagaat gaaactgaga tcaggagata ggcagttatt gcggtaaaat
    13921 cccagaacag cggtgagttg aaattgaccc acagggggcc ccgggtgttg ggatacggaa
    13981 taatgtagaa ggccagccat atacggccca tgtgtatcag gggaaacagg gcggcacatg
    14041 cgacagccag cactgtcatt gcttcagccg aacggttgat ggatgttctc cacctttgtc
    14101 tgagaacaag aaggaaaatt gaaaaggccg ttcctgcatg acctattcct atccaccaga
    14161 taaagttgat aatgtcccat ccccaggcca cggagttatt cagtccccag gtaccgattc
    14221 ccctggttac cgtgatgtac atggcataaa ccccgtaaac agctgcagcc gaggagacgg
    14281 ccaaagcaat ataccaccac agtccgggcc tggtttccag gggtgcaaca acctccttcg
    14341 ttatctggcc gaaagtcttg ttcccttcaa tgagcggacc tctgattttt ctgttataca
    14401 tcgcttgccc tgtattattt atgattttat atcctcgtca ttccacacct ttgtcagaaa
    14461 gcccaccgag ggcagtgtgt gcagttcctc aagcaggaaa tactgcctgg gatcactgaa
    14521 caggcgcgaa accctgctgt ttttgtcatt caggttgcca aacacgattg cccctgaagg
    14581 acaggcctgc atacatgctg tctttatctc accgtcattc agcgttctgt tttccagctt
    14641 tgccctcagc tttttttcct gaatacgctg aacacagaaa gaacatttct ccaccacccc
    14701 tctctccctt actgtcacat cggggtttaa aaccattttc cccagatccg atgccgtatt
    14761 gaaatcaaac gacctgttgt cggtatacct gtaccagttg aatctcctaa ctttataggg
    14821 acagttatta aggcagtact tcgttccgac gcaacggttg tatgtcatct ggttcaggcc
    14881 ttcactgctg tggttggtag ctgataccgg acatacgttt tcacaggggg cattatcaca
    14941 atgctgacac atgaggggct ggaaataaac cctgggattg tcggatgatt cggaataata
    15001 acggtctatt ctgatccagt gcatgattct ccgcagcctg acctcatcct ttcccacaac
    15061 gggaatattg ttttcggcct ggcaggcaat cacacatgca ttgcatccga cacatgcatt
    15121 gaggtctatt gctattcccc agtgaaatcc gtcatacttc acttcgggat aaagagtctt
    15181 gtcatgcaga tcagatgccc cgtgaagatt attccccgaa gccgggtttt ccctgtacct
    15241 gctcagaaaa gtttctttta caatcggcct tccttccatg gaggaatgtg tttgcgtgag
    15301 ggccagggga ctttccctta atgtattctc cacacggctg acagtaaatc cgtaactccg
    15361 gctgcctccc ttgatttttg caaagggata gaggttcact cctttcccgt ccgccacggg
    15421 tccggaaccg gtatgtccgt aaccggcagc cacggaaatt gttccttcag cctgtcccgg
    15481 ctggataaat gccggaagaa gaagtccgtc gccgattttc agtaaatgtc cgttcacgac
    15541 tccgagccct gcagcatcgg atggtgaaag acaggccaca ttttcccagc agtggcgggt
    15601 gagcggatca ggcaattcca tcagccacgg attgttggca tatattcccg tacccatgcc
    15661 tatgctttca taaataatga cctcatagtt atccggagaa ggaaggtccc tgctttccga
    15721 aaccttccgg aaggctgtcc ggtcaaaggg aggcatgttt tccgccttaa cgggacaggt
    15781 atggacaccc ttgcccaggg atgtgttcca gaaattcctg aatgaggact gacctgatcc
    15841 ggggaaatat tccctctccc agtaagaacg catgtagtca tgccatgtca cgggatttcc
    15901 tgaccatttc agaagggatt cctggaatga gcgtgtgctg aaaagcggat gaatcgcagg
    15961 ctggcaaagg cttacctgtc cgggaatgat ctcggcatcg ttccatgact caaggtaatg
    16021 atgaaccgga cactcatatt ttgccctgcc ggctgtctca ttgagagcaa ctgccatatt
    16081 gatggtcagt tccgttctgc tcagtccgtt caggaactta tccgcttcgg gataatcata
    16141 aacaggattg acattgtaca ttagcagggc cctgattttg ccgtcattca tatcatttac
    16201 cagagcctcg gtcttactgt caatgccggc agcaattgct aaatgcctcc cggggtcgat
    16261 acaatcagga taattgccaa gcaggtggtt aatcccgttt acaatgatct gtatctcaac
    16321 tttattagtt cctgatacca ctatggatct tcccctgcat gccatcaggc tttccgccag
    16381 ccccgacaga tcctcgcgga atgccggtcc cggaagtgtt tcagcaccag agtttacggc
    16441 aatcctgttg taaaggtcag taagcatgat cttttcctcg gacggtctta atttccttct
    16501 caggtcggca ttgcttcccg tgatgctcat acccgcctca taatgaatgt ggcggagcat
    16561 gctcctctca ccattgttca gtttcctgcg tgcaatatat ttgggaatga aatgaaccgg
    16621 agccacccat gtccccagga aatccgcatt tactgaaagc accaggtctg tcctgtcaaa
    16681 attataatcc gggatcactc ttttcccgaa acaaagggaa ttggcttcaa gaacagctga
    16741 atatttgtgc cagaccagca gtagctgcag ggttaag
//
LOCUS       DAOSPX010000029        16526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3600_length_16526_cov_3.904681, whole genome shotgun sequence.
ACCESSION   DAOSPX010000029 DAOSPX010000000
VERSION     DAOSPX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3600_length_16526_cov_3.904681"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="PLZ75_02690"
     CDS             complement(<1..1395)
                     /locus_tag="PLZ75_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HOS71338.1"
                     /translation="MDEARLLRFPAISGDKIVFSYAGDLYSVSSDGGIARKLTTDTGD
                     EIFPRFSPDGRYIAFTGQYNGNTEVFIIPGEGGTPRRLTHTATLSRDDIGDRMGPNNI
                     VMTWTPDGKHIVYRSRRHSFSDFLGQLFMVPLEGGMSKEIPLTNGGFCSFSPDGKKLA
                     FNWVFREFRTWKYYEGGMADDIRIFDFESGNVEKITDTRAQEICPMWIGEEIFFLSDR
                     DRTMNLFVYNTRTKETGKVTDFDTYDIKFPSSDGNQIVFENGGYIYRMDARTRKPEKV
                     TIYIADDFESAKRVTKDASERITMVSPSPDGERVVISARGDIFDVPVKEGVTRNLTAT
                     SGVHERNAQWSPDGKNIAWVSDAGGEFEIWMQKADRSMPARSITKDQNTYIFGIRWSP
                     DSKKLLYTTKKNDLRYVDVSTGRITVVDQSPKSPMYNASWSPDSKWIAFVRNEVGFPV
                     IRIYNVETAKSSEVT"
     gene            complement(1551..2273)
                     /locus_tag="PLZ75_02695"
     CDS             complement(1551..2273)
                     /locus_tag="PLZ75_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOS71339.1"
                     /translation="MCFTVNVNLIKEEIEYRYGINFPDKYRYEPSYYYHAFSFPELAA
                     ICSGYSTVRFLKWGLIPSWVRNASEAASVRTKTFNARSESLDSKQSFSSSFKSKRCII
                     PVRGFYEWQHAGKMKIPWYVYRADGGILSLAGLYSSWYNKDNGEIVDTCSVITTDANE
                     MMAVIHNSRKRMPVVLDRNNEENWISPDSSPSLLSEMLRPCPADVLKAHTIGPLINDR
                     TKVRNTPEIIREYIWDIPHTLF"
     gene            complement(2273..3607)
                     /locus_tag="PLZ75_02700"
     CDS             complement(2273..3607)
                     /locus_tag="PLZ75_02700"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOS71340.1"
                     /translation="MKSRYPLSVHKKILVIALLITLVSYNPGTGDESPELTNKLMLDN
                     VRLTNSGSDGPQFEDVERYLESFMRKWEIRGASVAVSKDGQLIYARGFGLADTVTKEE
                     VQPFYKFRIASISKLITAVAVMKLAEEGRLSLSDRVFGGNAILDDVYFSNPKDKRVYD
                     ITVAHLLSHEAGWSQRWGDQMFMPVVIADHMGTDMPVDTRTIIRFALDKNLHFTPGTG
                     RAYSNLGYAILGLVIEKISGMPYDEYCKKSILEPLGIYDMMLARNLKEEKAPWEVTYH
                     VPEDVLPKPSVYGTGELLSPCYGGNDIEALGAAGAWLATAPDLMRLLLAVDGFDTRPD
                     ILSAPSIELMTDNGKGLAPAGWKTTLDNGTWWRTGSFPGTAAMMKREADGTAWVVLLN
                     TSTWNGPEIHSYINSLMQRVTSKLNPGDNTDLFNFSIPVPLVSADGNELFIG"
     gene            3751..5187
                     /locus_tag="PLZ75_02705"
     CDS             3751..5187
                     /locus_tag="PLZ75_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOS71341.1"
                     /translation="MPKKIPIRRFVLAAILTCFVLHTGAQQVAEPDTSEYLPLIFNGA
                     LEYNLTIAAASGYSSEIERLIKAGADVDAKSSQGATPLFFAIINKRAEAVKTLVDYGA
                     NVNELSLYNENPLLLAINVNDLHIAEILIRNGADVNFKNRAGVTPLHVTSVYGLLELT
                     DLLLYYDAEVDAKDNDGTTPLMAAVWTGYSDVTGLLIVNGANMESRDNEGFTPFLIAA
                     QNGDTLIMNMLADRGVDIYARNIYNWDALALAIKSNHVKAAEFLLKKGNRWHEEARES
                     VNPYNIAAKYGRTEILELLEREKVPGRYKPRFDQMALSFSSRFNGKDFYSGMSLSFKE
                     PLSRAGLIAGIDTKLWYTRVLLKKTDDLFFQYFDRSSLVYAGAFREFRLTDNVLRGNI
                     FIHASLAAAYSFGNKLKGTEITPENKLRIMPSASVRWEKRILQVFGGMEYAGSPFYKT
                     GPLWVRIGCSVNFYFDPDRAPGKIIKWY"
     gene            5190..5642
                     /locus_tag="PLZ75_02710"
     CDS             5190..5642
                     /locus_tag="PLZ75_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71342.1"
                     /translation="MIKTYRIFFVFLLFTIVFSCEEVELEPGTMTLVNCSECTAEEPL
                     SAYITMIFRNPYELGVTGGMISVDIYEGNLEDNVIFKSIQVLRTEVEVNLPVNKKYTF
                     AASYYIDNKTYVVVNSIRPKVKFDKNSCDEPCYYTSPRKVNLKLRHTK"
     gene            5710..6309
                     /locus_tag="PLZ75_02715"
     CDS             5710..6309
                     /locus_tag="PLZ75_02715"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOS71343.1"
                     /translation="METSFFNSVIFSYGILPAFIFIARVCDVSLGTLRIIFVSKGNKK
                     IAPFLGFFEVLIWIVAISRIMQNLDNYLTYIAYAGGFATGNYVGMLIEERLAMGIQMI
                     RVFTHKNSEELVQTLNGRGYGATSVQAHGAKERVHLIYTIIQRNEMEEVLDIINKFNP
                     KAFYTIEDVKAVNEGIFHPRKRKNVIPLSNILREWRKGK"
     gene            6306..7172
                     /locus_tag="PLZ75_02720"
     CDS             6306..7172
                     /locus_tag="PLZ75_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71344.1"
                     /translation="MNRFSSHPLYRKHDLDSAMSTLWTFYRERFMILFITSFIMSLGV
                     QLVTLFFDFSELVNLTDPMEVLDKLSGMIWPMMAVTLLSLLCITILHYYVIYSPLDSE
                     VTIFVSLHRSLRYYLPYLIIMILFSVFASMAMVLGLMLLVIGVVFALVYVMTLYLFFM
                     PVLMAEGPDIANAISRTFILAHRRFWSNIGWVAVFLLILIVVSMILNSVIMIPFTGSM
                     LKSITKPEEALNAVSFIQNPVYIVLSSLVNALFYPLIPLLGTILYFNGRAREEAVSEQ
                     EQVQDQDSGTWS"
     gene            complement(7123..8268)
                     /locus_tag="PLZ75_02725"
     CDS             complement(7123..8268)
                     /locus_tag="PLZ75_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOS71345.1"
                     /translation="MRIKNILRFFLPVICFLTFSCLLTAQASGSDFEKMEQERTDLYA
                     GQLEDYLRMYLVDRYGERSGCLWNRDYSCEEAFVRSVEPNRERWKSLVIKPPVLKKTG
                     SVNRLPYTVEGIEGEWLELPLGPLTAQAFLAFPPGTDKNKPVPLVIAQHGIGSTPESP
                     YKGGGYNYYAKELLKAGFAVLAPMNLRSVERRNRIERLCRLADISLPGIELARVQHLL
                     DVVLEDARIDSERIGMWGLSLGGMATMFWMPLEPRIKAGIVSAWFNHRRNKMAVPDER
                     YVSFLVTAEEHAFFTGWLTEFSDHDVVSLICPRPLMIQHGKTDRIAWWPQVIEEFNVA
                     KAHYEKLNIPERMEIDVHEGGHDAVIISGIRFMTRYLNPDPAPVPVR"
     gene            complement(8293..9708)
                     /locus_tag="PLZ75_02730"
     CDS             complement(8293..9708)
                     /locus_tag="PLZ75_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71346.1"
                     /translation="MNKEKNTTSRRKFMQTTGMAAAGVAFGTNSFNAFSYDRIPGANE
                     KIRVGFIGTGNRGSQMLATFMGMKDCEVGALCDIYEPYITRDRAKVDPRYIRDMPGFI
                     PAMGETFPGKVERYTDYRKLLENKSIDAVCISTPDHWHALQTIHSIQAGKDVYVEKPF
                     SKTIREGRAMVRAAAESKQVVTAGLNRRGASTFMQLAKDIPAGKIGKVTFASACHVSN
                     MYPSGIGKMKPENPPADFNWDAWLGPRAYRPYQYNIAPYKFRWWEDYANQISNNGVHY
                     LDLIRWLIGETAPVTITATGGRYAVDDDRTIPDTMQATFEFASGVIVTVNIIETSSGS
                     FIPYGFLEIRGTKATLLTGENDYRIVPAKPGQFQTWTALAESETVDLQTDDKVFLSDG
                     RYRNSTANLVRNFLDCIRSRQATYCPPEEGHRSTSLAHLATIALITKERLEWDGSAER
                     FTNSEKANQLLEYEYRKPYKF"
     gene            complement(9766..10470)
                     /locus_tag="PLZ75_02735"
     CDS             complement(9766..10470)
                     /locus_tag="PLZ75_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71347.1"
                     /translation="MGGLFLLLLLAGCLIMRTRGRTLIQVDIHQNKELIYLSAFAEPP
                     QFAVWLENPGTGDLKNIFVTRRVATGDWEGKANVPVALPQWFRLFRENDNPSGSSGKA
                     DDLVISGATPKADYFSVRVEVKPESEWICWIEMNLAGDFNEAFPEFDPGTFREDEFGN
                     GQPALLFKTEVRAVEKNNYTAALWAQAVWNDGSVKIEPVSEGVTTARYVFDTINVSIL
                     RPKPKLIDKYKIREPE"
     gene            complement(10523..11782)
                     /locus_tag="PLZ75_02740"
     CDS             complement(10523..11782)
                     /locus_tag="PLZ75_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71348.1"
                     /translation="MSDVNDKVVLALIGAGGRGTQVILSMQKCSPGVEVKYVCDVDEE
                     RGGRAIDELEKQQGRRPERIGDMRLSFDDKDVDAVVICTPEHWHALATIRACQAGKDV
                     YVEKNISMTIPEGRKMIEAGKKYNRIIQCGFQNRSAPYNFSARNYIREGKLGKIVLVK
                     AYCMLPGHKPWIYKENEPVPDGLDWDQWLGPAPLVPYNVSRHKGYNEFWAYSCGLPLA
                     DCCHVIDLARMVLGDPAYVKSAYCAGGRVLYDDERDIPDNQTITYDFGEFPMTVEASI
                     YGEYMSKAAPEIRYGNLFPNWPFNSDRMEIYGTEGMMYLGRHGAGWQVLGSGSEIMAQ
                     EYGYFPDEAHQQDFINCIRSRKVPNSDIEQGHRSATLVHLANLSYRTGKKQLCFDPVS
                     ETLINSDEANRISLGTYRKNYEIPEII"
     gene            complement(11801..13141)
                     /locus_tag="PLZ75_02745"
     CDS             complement(11801..13141)
                     /locus_tag="PLZ75_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71349.1"
                     /translation="MRRRKFISAALTGSAGLAVGAGGLLMSSCKGANDRVVLALIGAG
                     GRGLSTISACCRENDGVEIKTICDVNDLNSARAAAEIEKQFGYRPQTVRYMNEIFADR
                     DIDAVYISTPDHWHALATIRACQAGKDVYVEKSPSHSIREGRKMAEAAAKYRRIVQVG
                     FQNRSAGYNHEAREYISSGRLGQVVHIRIYNLLPAGRWIPIPEEAVPPTLDWNEWIGP
                     APFRPYSPALLKGWVYFYDYCPGFLDDAVHQFDLSRMVMGDPGHPQSVYGWGGKHVWN
                     SEQQAPEVQSVTFDYDKYTLTCESGNVTAYMSKTPDDIRMDPSRFPEWRTNADRIEIY
                     GTSGVMFLGRQGGGWQVYGPKEEIVAQSGGIHSDREHQINFIECVRKRKQPNSTVVQG
                     HMSATLAHMANIAYRCGNRQLLFDAGNERFIDNEEANRLLKGSYRENYIVPEKV"
     gene            complement(13352..14731)
                     /locus_tag="PLZ75_02750"
     CDS             complement(13352..14731)
                     /locus_tag="PLZ75_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71350.1"
                     /translation="MTRKLLIILCLSAAIAGCRNETRENPASLPVSTPDARINATVYD
                     SGFEHSHDTYNGLSSASDGKIYYVLCSELMDVAGQFYCLDPETGEIEHLGDLTELCGE
                     KDMKVVAQGKCHTNFHEMQGKLYFSTHLGYYSIIDGMEKPGIPEKGYGPYRGGHLLAL
                     DLKTRKTEDLGIAPDKCGILTMGMDTVRGLIYGLTWPLGNLFRYNVFSREMEDLGPFT
                     GRGEAGKGQEYRVICRSFAVNHDDGSVYLTNAEGDIKRLRPGQNGVETIAEEDMRKDY
                     FGTYEIFTSGSMAYNWRQVFWHEPEKKIYGVHGNSGYLFRFDPEIPEMEVLERITSIP
                     SKRTGMGDQFSYGYLGFKPGPDGHTIYYLTGAPVYIEGKRVRGAATTSRGEAKGLEDL
                     HLITYDINNGLYLDHGAVFYPDGQRPLYVNSIAIGNDGTVYSMGRINRNGKTVTDLFS
                     IPDPFKKYD"
     gene            complement(14756..16129)
                     /locus_tag="PLZ75_02755"
     CDS             complement(14756..16129)
                     /locus_tag="PLZ75_02755"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS71351.1"
                     /translation="MSEKNNYSRRKFVKQNTIAGVGAAVAMSVTPSVFAGCAADTGIP
                     AILGGTKIRTGEWPSWPLWDPAADEEQVIRVLRSGVWSRAEMVTRFEETWAELIGSRR
                     CLAVVNGTNALITALAQSDIGGGDEVIVPPYTFIATVIAVLQTGAMPVFVDTDPETFQ
                     IDVNKIEEKITPRTRAILPVHILGLPADMKRIMTIARKHNLVVIEDACQAWLAEIDRR
                     KVGTFGKAGCFSFQNSKNIPIGEGGAIVSDDDEFMDRCYSYHNYGNPYGSHVGTISSG
                     TLIAGTKQRLTEYQAAIGLAQLQRLEEQTEKRNRNAAYLRSEIRRIPGILPYKLTPGV
                     TRCAFHLFPFRYRKEEFSGLSREQFLKALDAEGIPAMSGYTPLNKMPYMKNVLESKNF
                     RLMYTPEMLDYDKYMDNNQCPENDRLCEEAVWLFHPLLLAEKKDMDDIADAIWRISRN
                     ADKIKNL"
     gene            complement(16224..>16526)
                     /locus_tag="PLZ75_02760"
     CDS             complement(16224..>16526)
                     /locus_tag="PLZ75_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS71352.1"
                     /translation="LTLLTRSMTCEWAKYNIQANGIGPGYIATAQTAPIREDGHPFNE
                     LVMTRTPAGRWGRPEDVANAALFLASEASDFVNGHILFVDGGILANFGYVKGENEI"
BASE COUNT         4219 a         4064 c         3541 g         4702 t
ORIGIN      
        1 agtcacttcc gatgattttg ccgtttccac attataaata cggatcacag gaaaccccac
       61 ctcatttctg acgaaggcaa tccatttgct gtcaggcgac cagctggcat tatacatcgg
      121 actcttgggt gattgatcca caacggttat cctgcctgtg gaaacatcca catacctgag
      181 atcattcttt ttagtcgtgt aaagaagttt tttactgtca ggcgaccatc tgatcccgaa
      241 aatatatgtg ttctgatctt tcgttatgct gcgggccggc atggatctgt cggctttctg
      301 catccatatc tcgaattccc cgccggcatc ggaaacccag gcaatatttt tcccgtcagg
      361 agaccactgt gcatttcttt catgaacacc tgaagtagca gtgagatttc ttgtcactcc
      421 ttcctttacc ggaacgtcaa agatatcgcc gcgagcactt ataactactc tttccccgtc
      481 gggagaagga ctgaccatgg taatcctttc cgatgcatct ttcgtcactc ttttcgctga
      541 ctcaaaatca tctgcaatat aaatggtcac tttttcgggt ttgcgtgtcc gggcatccat
      601 cctgtatatg tagccgccat tttcaaacac aatctggttt ccgtcactgg acgggaattt
      661 aatgtcgtat gtatcgaaat ctgttacttt tccggtttct tttgtacggg tgttgtatac
      721 aaacaggttc attgtcctgt cccggtcaga aaggaaaaat atctcttctc ctatccacat
      781 gggacatatt tcctgggccc gggtatctgt aattttttcc acattgcccg attcaaaatc
      841 aaatatccgg atatcatcag ccattcctcc ctcataatat ttccaggttc tgaattcgcg
      901 gaaaacccag ttgaatgcca gttttttccc gtcaggcgaa aatgagcaga atccgccgtt
      961 tgtcaggggt atttcctttg acattccacc ttcaagcggt accatgaaga gctggcccag
     1021 aaaatcactg aaggaatgtc ttcttgacct gtaaactata tgtttcccgt caggagtcca
     1081 ggtcatgaca atattgtttg gccccatcct gtcgccaatg tcatccctgc tcagggttgc
     1141 tgtatgtgtc agcctcctgg gagtacctcc ctccccggga ataatgaata cttcagtatt
     1201 tccgttatac tgtccggtga aggcaatata tctgccgtca ggcgaaaaac ggggaaatat
     1261 ttcatctccg gtatcagtag tcagttttct ggcaattcct ccgtcagaag atacagagta
     1321 caggtcgccg gcatagctga acactatctt atcgccgctg atagccggga agcgcagcaa
     1381 tctggcttca tccatcgctg acaatgataa atataaggct aacagaataa tggaagtgaa
     1441 aaatttagaa gtcataattt taagtttaag ttcagggaca atccggcaat ttagtaaaaa
     1501 aaccccaatc catggcatat ttcgccgtaa ataaacggaa ttgatataat ctagaatagt
     1561 gtatgaggga tatcccaaat atattccctt attatttccg gagtattcct gactttggtc
     1621 cggtcattta tcagaggacc tatggtatgg gctttcagca catccgcggg acagggcctg
     1681 agcatttccg acaagaggga cggtgaagaa tccgggctga tccagttttc ttcattattc
     1741 cggtccagca caacgggcat tcttttcctg gaattatgga tcacggccat catttcgttc
     1801 gcatcagtgg ttataaccga acaggtatcc acaatctcgc cgttatcctt attgtaccag
     1861 ctggaatata gtccggccag tgaaagtatc ccgccgtctg ccctgtaaac ataccagggt
     1921 atcttcatct ttccggcatg ctgccattca tagaaccccc tgacgggaat gatgcatctt
     1981 tttgatttga atgaggatga aaaagattgt ttcgaatcca ggctttccga tcgtgcattg
     2041 aatgtttttg tccttacaga ggctgcctca gatgcatttc tgacccagga aggaatgagt
     2101 ccccatttaa gaaaacgcac cgttgaataa cccgaacata tggccgccag ttccgggaaa
     2161 ctgaaggcat ggtaataata gcttggttcg tagcggtatt tatcaggaaa attaatcccg
     2221 tatcggtatt caatttcctc ctttatcaga ttgacattaa ccgtaaagca cattatccaa
     2281 taaataattc atttccgtcg gcagatacaa ggggtaccgg aattgaaaaa ttaaataggt
     2341 cggtattgtc tccggggttt agctttgatg tgaccctttg cataaggctg ttaatgtatg
     2401 aatgtatctc gggtccgttc caggtgcttg tattaagaag aacaacccag gccgttccgt
     2461 cggcttccct tttcatcatt gcggctgttc cgggaaagga gccggtccgc caccatgttc
     2521 cgttatccag ggttgttttc catccggccg gtgcaagacc cttgccgtta tctgtcatca
     2581 gttcaatgct aggggccgac aatatatccg gccttgtatc aaacccgtca accgcaagca
     2641 acaggcgcat caggtcggga gccgttgcaa gccatgcacc tgccgcaccg agagcctcaa
     2701 tatcatttcc tccgtagcag ggagacagga gttctcctgt tccataaacc gaaggttttg
     2761 gcaggacatc ttccggaaca tgataagtca cttcccaggg tgctttttct tccttcaggt
     2821 tgcgggcaag catcatgtca taaattccaa gcggttccag gattgatttt ttacaatatt
     2881 catcataagg catgcccgat atcttttcaa tgaccagtcc cagaatggcg tatccgagat
     2941 ttgaataagc ccttccagtt ccgggagtaa aatgaaggtt cttatcaagg gcaaagcgga
     3001 taatggtcct ggtgtccacg ggcatgtccg ttcccatatg atcagcaatt accaccggca
     3061 taaacatctg atcaccccag cgctgcgacc atccggcctc atggctcagg aggtgtgcca
     3121 cagtaatatc atataccctt ttatcctttg gattactgaa ataaacatca tcaagaatgg
     3181 cattcccccc gaaaaccctg tcgcttaaag acaggcggcc ttcctcagct agtttcatca
     3241 cggcaactgc cgttatcagc tttgagatac tggctatccg gaatttataa aatggctgca
     3301 cttcttcctt tgttacggta tcagccagtc caaatcccct ggcgtaaata agttgaccgt
     3361 cctttgatac tgcaacggat gcacccctta tctcccattt tcgcatgaat gattcaaggt
     3421 atctttccac gtcttcgaat tgcggcccgt cagaacccga atttgtcagc cgcacattat
     3481 ccagcatcag cttgttggta agttcagggc tttcatcacc ggttccggga ttataactta
     3541 caagagttat cagaagtgca atgaccagta tttttttatg aactgacaaa ggatatcgac
     3601 ttttcaatca gcaaaaataa aactattcct gtaactattc cggccgtttt gcctgtttta
     3661 ataaaaatca gacggaaatt attcacattt tgactgtgaa ccattgtttt tacacctctg
     3721 aaaattcaat caaagaatta actttgtact atgccaaaaa aaattccaat acgccggttt
     3781 gttttagcag caattctcac atgttttgtt ctgcatactg gagcacaaca ggttgcagaa
     3841 ccggatacct ctgaatacct tcccctcatt tttaacggag ccctggaata taatcttacc
     3901 atagcagctg cttccggata cagttcagaa attgaaagac tgataaaagc cggagctgat
     3961 gtggatgcaa aaagcagcca gggagccacc ccgctttttt tcgccataat caacaaaagg
     4021 gcggaggcag taaaaacact tgtcgattac ggggcaaacg ttaacgaatt atcactgtac
     4081 aacgaaaatc cgcttcttct tgccataaat gtcaatgatc tgcatattgc tgaaattctc
     4141 ataaggaacg gtgcggatgt caactttaaa aaccgcgcag gtgtcacgcc attgcatgtt
     4201 acatcggttt acggcctcct tgagctgacg gatctgcttc tttactatga tgcagaagtt
     4261 gatgcaaagg acaatgacgg caccacgccg ctgatggctg cggtctggac cggttattca
     4321 gatgtgaccg ggctgctcat tgtaaacggg gcaaatatgg aatcaaggga caatgaaggt
     4381 ttcacaccat ttctaatagc cgctcagaat ggcgacacgc tgattatgaa tatgctcgca
     4441 gacaggggtg ttgacattta tgcaaggaac atatacaact gggatgcgct ggcacttgca
     4501 ataaaatcaa atcatgtaaa agcagcggaa tttcttctga aaaaaggaaa cagatggcat
     4561 gaagaagcca gggaatcagt taatccctat aatattgctg caaagtacgg gagaacggaa
     4621 atacttgagc tgcttgaaag ggaaaaagtt ccaggacgct ataagccccg tttcgatcag
     4681 atggctctgt cattttcttc cagattcaac ggaaaggatt tctacagcgg aatgagcctc
     4741 tcattcaagg aaccacttag ccgtgccgga ctgattgccg gaattgatac gaagctgtgg
     4801 tacaccagag tacttttaaa aaagacggat gatctgtttt tccagtattt tgacaggagc
     4861 agtctggttt acgctggtgc attcagggaa ttccggctta cagacaatgt attgaggggt
     4921 aacatattta tccatgcatc cctggctgca gcctattcct ttggcaacaa gctgaaaggg
     4981 actgagatca ctccggaaaa caagctccgt attatgccct cggcatctgt cagatgggag
     5041 aaaagaatat tacaggtttt tggcggaatg gaatatgcag gatcaccctt ttataaaacc
     5101 ggcccattgt gggtaagaat cggatgttct gtgaatttct actttgaccc ggacagggct
     5161 ccgggaaaaa tcataaaatg gtactgatta tgataaaaac atacaggata ttctttgtat
     5221 ttctgctttt caccattgtc ttctcctgtg aagaagtcga gctggaaccg ggtacaatga
     5281 ccctggtaaa ctgcagtgag tgcactgcag aagaacctct gagtgcgtat atcaccatga
     5341 ttttcaggaa cccctatgag ctgggagtaa ctggcggaat gatttccgtg gacatttacg
     5401 agggcaacct ggaagacaat gtgatattca agtcaataca ggttttaaga acggaagtgg
     5461 aagtaaattt gccggtcaac aaaaaataca cctttgccgc cagctattat attgataaca
     5521 agacctatgt tgtggtaaac tccatcagac caaaagtaaa attcgacaaa aacagctgcg
     5581 atgaaccctg ttattacaca tctccgcgaa aagtgaatct gaaactcaga catacaaaat
     5641 aactgttata ataatgcaga ttaatgaata atcaggcatc gactttgtaa atattaaaca
     5701 atctcacaaa tggaaacctc ctttttcaat tctgtcattt tcagttacgg tattctgcct
     5761 gcatttatct ttattgccag agtctgtgat gtttcacttg gcacactcag aataatcttt
     5821 gtatctaaag gcaacaagaa aattgctccc ttcctgggct tttttgaagt tcttatctgg
     5881 atagtggcaa tcagcagaat aatgcagaac cttgataatt acctgaccta tattgcatat
     5941 gcgggtggtt ttgctaccgg aaattacgtg ggcatgctta ttgaggaaag gctggcaatg
     6001 ggaattcaga tgataagggt gtttacgcac aaaaattcgg aagaactggt ccagacattg
     6061 aacggcaggg gctacggagc cacttcagta caggctcacg gggcaaagga gagagtccat
     6121 ctcatttaca ccattattca aagaaacgaa atggaggagg tcctggatat tattaacaag
     6181 ttcaatccca aggcatttta tacgattgaa gatgtgaaag ccgttaatga aggaattttc
     6241 catcctagaa aaagaaaaaa cgtaattccc ctgtcaaaca ttctcagaga gtggaggaaa
     6301 ggaaaatgaa caggttcagc agccatcccc tttacaggaa acatgatctt gacagtgcta
     6361 tgagcactct gtggaccttt tacagggaaa gattcatgat tctgttcatc acatccttca
     6421 tcatgtcatt gggagttcag ctggtaacgc tgttttttga cttcagtgag cttgtgaatc
     6481 tgaccgatcc gatggaggtg ctggacaaat taagcgggat gatttggccg atgatggctg
     6541 tcactcttct gagcctgctc tgtattacta ttttgcatta ttacgtcatt tacagtcctc
     6601 ttgacagtga ggttacaatt tttgtatctc ttcacaggtc attgagatat tatctgccct
     6661 acctgataat catgatcctt ttttcggtat ttgcatcaat ggccatggtt ctgggactta
     6721 tgctgcttgt tataggtgtt gtttttgcgc ttgtctatgt catgaccctg tatctcttct
     6781 tcatgcctgt actgatggct gaagggcctg atatagccaa tgcgataagc cgcaccttca
     6841 ttctggccca caggaggttc tggtcaaata tcggctgggt tgccgttttc ctcctgatac
     6901 tgatagttgt ttcaatgatc cttaactccg tcataatgat tcccttcacg gggagcatgc
     6961 ttaagagcat aacaaaacca gaagaggcat taaatgcggt cagttttatt cagaatccgg
     7021 tatatattgt actatcctcg cttgtaaacg ctttattcta cccgctcatt ccgctgcttg
     7081 gtaccatttt atactttaac ggcagggccc gggaagaagc agtcagcgaa caggaacagg
     7141 tgcaggatca ggattcaggt acctggtcat aaaacgtatc ccgcttatga tgacagcatc
     7201 gtgacctcct tcatgaacat caatttccat tctttcaggg atattcagct tttcataatg
     7261 cgcttttgcc acattgaatt cttcgattac ctgtggccac caggctattc tgtctgtttt
     7321 cccgtgttgt atcatcaggg gcctgggaca aatgagtgaa acgacgtcat gatcggaaaa
     7381 ttcagtgagc cagcccgtaa agaaagcatg ctcctcggct gttaccagga agctgacata
     7441 tctttcatcg ggaactgcca ttttattgcg acggtgattg aaccatgcgg aaactatccc
     7501 tgccttaatc ctgggttcca gtggcatcca gaacatggta gccatgcccc caagtgaaag
     7561 tccccacatc cctatccttt cagagtcgat ccttgcatct tccagcacca catcaagaag
     7621 atgctgcact cttgccagct caatgcccgg aaggcttata tcagccaggc ggcacagcct
     7681 ttcaatacga tttcttcttt caacagatct caggttcatg ggggccagca ctgcaaaccc
     7741 ggctttcagc aattccttcg cgtaataatt ataaccaccc cccttatagg gagattcagg
     7801 agtactgcca ataccatgct gtgcaattac aagcggtacc ggtttatttt tatccgttcc
     7861 gggcgggaaa gcaaggaaag cctgagctgt aagcggtccc aaaggcaatt ccagccactc
     7921 accctcaata ccttccaccg tatatggcag cctgttcacg gaaccggttt ttttcagaac
     7981 agggggtttt atcaccaggg acttccagcg ttccctgttg ggttcaacgc ttctgacaaa
     8041 agcctcttca cagctgtaat cacggttcca cagacagccg ctgcgttccc cgtaacggtc
     8101 aacaagatac attctgagat aatcctcaag ctgtcccgca taaagatctg tccgctcctg
     8161 ctccattttt tcaaagtccg aaccggatgc ctgtgcagtc agcagacagg aaaaagtcag
     8221 gaaacagatg accggcagga aaaatcgtaa gatatttttt attctcataa taattggttt
     8281 acagagcgac ctctaaaatt tatatggttt cctgtattca tattcaagaa gctgattggc
     8341 cttttcgctg ttggtaaatc tctccgctga accgtcccac tcaagtcttt cctttgttat
     8401 cagggcaatg gttgcaaggt gggcaaggct cgtggagcga tggccctctt caggaggaca
     8461 gtaggtggcc tgtctggacc tgatgcagtc gaggaaatta cgaaccaggt tggcagtgct
     8521 gttacggtat ctgccatcgg aaagaaacac tttatcatca gtctgcagat caactgtttc
     8581 agattctgcc agtgctgtcc atgtctggaa ctggcccggt ttggcaggta caatcctgta
     8641 atcattttcc cctgtcagca atgtagcttt cgtacccctg atttccagga agccataggg
     8701 aataaatgag cctgaactgg tttcaataat attaaccgtc acaatcacgc cggaggcaaa
     8761 ctcaaaggtg gcctgcatgg tgtcgggtat ggtacggtca tcatcaacag catacctgcc
     8821 ccccgttgcc gttatggtta cgggggcagt ctcaccgatg agccagcgta taagatcaag
     8881 ataatgaacg ccattattgc ttatctgatt tgcataatcc tcccaccagc ggaatttata
     8941 cggggcaatg ttatactgat atggcctgta ggctcttggt ccgagccatg cgtcccagtt
     9001 gaagtctgca ggcgggtttt ccggtttcat ttttcctatg ccggaaggat acatattgct
     9061 tacatggcag gcactggcaa aggtaacttt cccgattttc cctgcaggga tatcttttgc
     9121 cagctgcatg aatgtggatg ctcctcttct gttgaggcct gccgttacaa cctgtttgct
     9181 ctctgcagca gccctgacca tggcgcgtcc ttccctgatg gttttggaga aaggtttttc
     9241 aacatagaca tccttgcctg cctgaatgga atggatggtc tgaagtgcat gccagtgatc
     9301 aggcgtggaa atacaaacag catcaatact tttattttcc agcagtttgc ggtagtcggt
     9361 atatctctct acctttccgg gaaatgtttc ccccattgcc ggtataaatc cgggcatgtc
     9421 cctgatataa cggggatcga cctttgcacg gtcacgtgtg atgtaaggtt catagatatc
     9481 gcaaagagcg ccaacctcac aatccttcat gcccataaat gttgcaagca tctggctgcc
     9541 gcgattgccg gtgccaatga atccgactct tattttctca tttgctcccg ggatccggtc
     9601 ataactgaag gcattaaaac tgtttgtgcc aaatgcaact cctgctgcag ccatcccggt
     9661 cgtttgcatg aatttcctgc gggatgtggt gtttttttcc ttgttcatgt tcaggttttt
     9721 aaaaagttaa aattttttat ccgtgacaga tcacatccgg taaaatcatt ccggctcacg
     9781 gatcttatac ttgtctatca gtttcggctt tggtctgagt atcgaaacat taatagtatc
     9841 aaacacatat cttgctgttg taaccccctc gcttacgggt tcaattttta cggaaccgtc
     9901 gttccaaacc gcctgagccc acaaagctgc tgtgtaatta tttttttcaa cagccctgac
     9961 ttcagttttg aacagtagcg cgggctgtcc gtttccgaat tcatcttctc tgaaagttcc
    10021 gggatcaaac tcgggaaatg cctcattaaa atcaccggca agattcatct cgatccagca
    10081 gatccattca gattcaggct tcacttccac cctgacagag aaatagtcag cctttggtgt
    10141 agcaccgctg atcacaaggt catctgcctt tccggatgaa ccggatggat tgtcattctc
    10201 cctgaacagc cggaaccact gcggcaatgc caccggaaca tttgcttttc cctcccagtc
    10261 gccggtagcc acacggcggg taacaaaaat attctttaaa tcaccggtcc cgggattctc
    10321 cagccagaca gcaaactgag gaggttcagc aaaggctgac agatagataa gttccttatt
    10381 ctgatgtata tcaacctgta tgagagtccg gccccgggtt ctcattataa ggcagccggc
    10441 aagcaacagc agcagaaaaa gaccacccaa agccactaat attatatttc tgaataactt
    10501 cttccgctga gggatcatag tttcagataa tctccggtat ttcataattc tttctgtaag
    10561 ttcccaggga aatccggtta gcttcgtcag aattaataag cgtttcactt acaggatcaa
    10621 agcaaagctg tttctttccc gtacggtatg acaggttggc cagatggacc aatgtggcgc
    10681 tcctgtgacc ctgttcaata tcggaattag gcactttccg gctgcggata cagtttataa
    10741 aatcctgctg atgagcttca tcagggaaat aaccatattc ctgcgccatt atttcggatc
    10801 cggatcccag aacctgccat ccggcaccat gacggccgag atacatcatt ccttcagtcc
    10861 cgtatatttc catcctgtcg ctgttaaagg gccagttcgg aaataagttg ccataccgga
    10921 tttcgggtgc tgccttcgac atgtattccc cgtatataga agcctccaca gtcatgggaa
    10981 actctccaaa atcatatgta atagtctggt tgtccggaat atcgcgctca tcatcgtata
    11041 acacccgtcc tccggcacaa tatgctgatt tcacatatgc gggatctccc agcaccattc
    11101 ttgcaaggtc aataacatga caacagtctg caagcggcaa tccgcaggaa taagcccaga
    11161 attcattata tcccttatga cggctcacat tatagggaac caggggagcc ggaccaagcc
    11221 actgatccca gtccagtcca tcaggaacag gttcattttc cttgtatatc cacggtttat
    11281 ggccgggaag catgcaatag gccttcacca gaacaatttt tccgagtttc ccttccctga
    11341 tatagtttct tgcagagaaa ttatatgggg cgctccgatt ctggaatcca cactgaatga
    11401 tcctgttata ttttttaccc gcttcaatca tttttcttcc ttcaggaatg gtcatggaaa
    11461 tatttttttc cacatataca tcctttccgg cctggcatgc cctgattgtt gcaagagcgt
    11521 gccagtgttc cggagtgcag atcactactg catcaacatc cttatcgtca aaggataacc
    11581 tcatatcgcc tatacgttcc ggtctcctgc cctgctgttt ttccagttca tctattgccc
    11641 ttccccctct ctcctcatca acatcacata catattttac ttccactcca gggctgcatt
    11701 tctgcatact cagaataacc tgtgttcctc ttcctccggc acctatcagt gcgagaacca
    11761 ctttatcatt tacatctgac atttctttcg cattttcaag ttaaaccttt tccggaacga
    11821 tataattctc acggtaactg cccttcagaa gcctgtttgc ttcttcattg tctatgaacc
    11881 gttcatttcc ggcatcgaag agcaactgcc tgttgccgca acggtatgca atatttgcca
    11941 tatgtgcaag tgtagcactc atatggccct gaacaacagt actgtttggc tgtttccgct
    12001 ttctgacgca ttctatgaaa ttaatctgat gctccctgtc ggaatgaatc ccgcccgatt
    12061 gtgcaactat ctcttccttt ggtccgtaaa cctgccagcc tccgccctgc cgtccaagaa
    12121 acatcacacc cgatgttccg taaatttcta ttctgtcagc attggttctc cattcaggaa
    12181 aacgcgacgg atccatcctg atatcatccg gcgtttttga catgtatgcg gtaacgttgc
    12241 cgctttcgca ggtcagggta tatttatcat aatcaaaggt tactgactgc acttcgggtg
    12301 cctgttgttc agaattccag acatgcttgc cgccccagcc ataaaccgat tggggatgac
    12361 cgggatcacc cattaccata cggctcagat cgaactggtg aactgcatcg tcaaggaatc
    12421 cggggcaata atcgtagaag taaacccagc cctttagcaa tgcaggagaa tatggccgga
    12481 acggggccgg gccaatccat tcattccagt ccagtgtcgg aggaacagcc tcttcgggta
    12541 tgggaatcca tctccccgca ggaagcaggt tatagatacg gatatgcacg acctgtccga
    12601 gcctgccgct gctgatatat tccctggctt catgattata gccggcactc cggttctgga
    12661 atcccacctg gactatccgc cggtactttg cggctgcctc tgccattttt ctgccttccc
    12721 ggattgagtg actcgggctc ttttcaacat atacatcctt accggcctgg caggctctga
    12781 ttgttgcaag tgcatgccag tggtcgggcg tggaaatata tactgcatct atatcccggt
    12841 cggcaaaaat ctcattcata tacctgactg tctgcggtct gtagccgaat tgtttttcga
    12901 tttcagcagc agccctggct gaattaagat catttacatc acagatggtt ttgatctcca
    12961 caccatcatt ttccctgcaa catgccgata tggttgacag ccccctgcca ccggcaccaa
    13021 tcagagccag aaccaccctg tcatttgctc ccttacagga tgacatcagc aggccgccgg
    13081 caccaacagc cagtcctgcc gaacctgtaa gtgcagcaga aataaatttt cgtctcctca
    13141 tgtttcattt tgttttatta ctattcaatt tccagaattg ttcctgcaat atgtctgctc
    13201 ccatccccga tattgaaata atataccacc agaatcttaa aaaatgattt tcccggatat
    13261 gtcatggaac gatttttcag atggaagtta ttatcgtccg gttcaaaata atcacaataa
    13321 atacaagtct gtttttcagt cgtatatgaa attaatcata ctttttgaaa ggatccggaa
    13381 tactgaacag gtctgttacg gttttcccgt tcctgttgat cctgcccatg ctgtatacag
    13441 taccgtcatt tcctatggcg attgaattaa cgtaaagggg tctttgtccg tcgggataaa
    13501 aaacagcccc gtgatcaaga tacagaccat tgttaatgtc ataggttatc aggtgaaggt
    13561 cttccaggcc ttttgcctct cccctggatg ttgttgccgc tccccttaca cgtttccctt
    13621 caatatagac gggagctccg gtaagatagt agattgtatg cccgtccggc ccgggtttga
    13681 aacccagata gccgtagctg aactgatctc ccattccggt acgttttgac ggaattgaag
    13741 taattctttc cagcacctcc atctccggga tttccggatc aaatctgaaa agatatccgg
    13801 aattgccatg aacgccgtaa atcttttttt ccggttcatg ccagaacacc tggcgccagt
    13861 tatatgccat gctgcctgaa gtaaaaatct catatgtacc gaaatagtcc ttcctcatat
    13921 cctcttctgc tatggtttcc actccatttt gtcccggtct cagccttttt atatcgcctt
    13981 cggcattggt cagatagacg gaaccgtcat catgattaac cgcgaaagaa cggcagataa
    14041 ccctgtattc ctgtcctttg cctgcttcgc ctctgcccgt gaagggacca agatcttcca
    14101 tttcacgcga gaacacatta tacctgaata gatttcccag cggccacgta agtccgtata
    14161 tcagtccgcg gaccgtatcc atgcccattg tcagtatccc gcacttatcg ggagctatac
    14221 ccagatcctc ggtttttctc gttttcaggt caagagccag aagatgacct cccctgtacg
    14281 ggccatagcc cttttccgga atacccggct tttccattcc gtcaataatg ctgtagtatc
    14341 ccagatgggt ggaaaaatac agcttcccct gcatctcatg gaaattggta tggcatttcc
    14401 cctgggctac aaccttcata tccttctcgc cgcataattc ggtcagatca cccagatgtt
    14461 caatctcccc cgtttcagga tcaaggcagt aaaactgtcc ggccacatcc atcagttcgg
    14521 agcagaggac atagtatatt tttccgtcgc ttgcagaaga caatccgttg tacgtgtcat
    14581 gcgaatgctc aaagccggaa tcatagaccg ttgcatttat ccttgcatca ggtgttgata
    14641 caggaaggga tgcaggattt tcccttgttt cattccggca tccggcaatg gcagcagaca
    14701 ggcacaggat gatcaggagt tttcttgtca ttatttgtga aattatattg ttgattcaga
    14761 gatttttaat tttatccgca tttctggaaa ttctccagat tgcatcagca atatcatcca
    14821 tatccttttt ctctgcaagg agtaagggat ggaaaagcca tacagcctcc tcacacagcc
    14881 ggtcattttc cgggcattga ttgttatcca tatacttgtc ataatcaagc atttcaggcg
    14941 tatacatcag tctgaaattt tttgattcca gcacattttt catatagggc attttgttca
    15001 ggggcgtata tccgctcatt gcaggtatac cttctgcatc gagggctttg aggaactgct
    15061 ccctggaaag accgctgaat tcttctttcc tgtaacggaa agggaaaaga tgaaaagcgc
    15121 atctggtgac accgggcgta agtttatacg gcaggatacc gggaatcctt ctgatttcag
    15181 atctcaggta tgctgcattt ctgtttcttt tttcggtttg ttcctccagg cgttgcagct
    15241 gtgccagtcc tatggctgcc tgatattccg tcagtctctg ttttgtgccg gcaataaggg
    15301 ttcctgaact tatggtacct acatgtgagc cgtatggatt gccgtaatta tggtaggaat
    15361 aacagcggtc catgaattca tcatcatcgc ttacaatggc tcctccttct ccaatgggaa
    15421 tattttttga attctggaaa ctgaaacagc cggcttttcc gaatgtaccg accttcctgc
    15481 ggtcaatttc agccagccac gcctggcagg catcttctat taccacaaga ttgtgctttc
    15541 tggcaatagt catgattctt ttcatgtcag caggcaggcc gagaatgtgt acaggcagaa
    15601 tggccctggt tctcggagtg attttttcct ctattttatt gacatcaatc tggaaggttt
    15661 ccggatcggt atcaacaaat acaggcattg cccctgtctg aagcactgct ataaccgttg
    15721 caatgaatgt atacggagga acgatgactt catccccgcc tcctatatct gactgtgcca
    15781 gtgcggtaat cagggcattt gtaccgttta caactgccag acatcgcctg gacccgatca
    15841 gttctgccca tgtttcctca aacctggtca ccatttcagc acgtgaccat accccgctcc
    15901 gaagaacacg gatcacctgc tcttcatcgg cagccggatc ccataagggc caggaaggcc
    15961 attcccctgt ccttattttc gttccgccca gaattgccgg aataccggtg tctgcggcac
    16021 atcctgcgaa aacggaagga gtcacactca ttgcgacagc agcccctact cctgcaattg
    16081 tgttttgttt cacaaatttg cgccgagaat aattattctt ttcactcatg acagcaatat
    16141 tttggtttat agttaaaatt cggaattatc atgcatttac aattaaaatg aacctcaaat
    16201 ccccaacagg acagcaggca gaatcagatt tcattttcac ctttcacata accaaagttt
    16261 gccagtatgc cgccgtcaac gaacagaata tgtccgttaa caaaatcact ggcttcagaa
    16321 gccaggaaca atgctgcatt tgccacatct tccggcctgc cccatctgcc cgcgggggtt
    16381 cttgtcatta ccagttcatt gaaaggatgg ccgtcctccc tgataggtgc tgtctgggca
    16441 gtggcaatgt atcccggacc gataccgttt gcctggatgt tgtatttcgc ccattcgcat
    16501 gtcatgctcc tggtcaggag ggtcag
//
LOCUS       DAOSPX010000030        15874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3797_length_15874_cov_4.647386, whole genome shotgun sequence.
ACCESSION   DAOSPX010000030 DAOSPX010000000
VERSION     DAOSPX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3797_length_15874_cov_4.647386"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3073
                     /locus_tag="PLZ75_02765"
     CDS             <1..3073
                     /locus_tag="PLZ75_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS71353.1"
                     /translation="INYFILFCILVSFPLIGYGQFEVRGKVTDSQSDQPLAGVTVVIK
                     GSTTGTLTDIDGNYSILAPSEKSILAFSFIGYISREVTVGNQKEIDIALETDVLRLEE
                     VVVIGYGTAKKSDLTGSVVSVKAENYKTQQMVQFTDMLTGTIAGFNANQGTSASGGGA
                     SLEIRGPKSLSAGTDPLIVLDGVIYTGTLREINPNDIASVDILKDASSAAVFGAKAAA
                     GVVLITTTRGRLGKPTINFSTKIGIAEVNNKRRGLGPEEYIRFRQDYFRQMFPSIDYN
                     FYTHPDKLPPGVSIDQWRALSSSPVDDNIQEWMGRMRFYPEEQENYREGRTMDMFDYV
                     FRKGLQQDYDLSISGGTEKATYYWSIGYANNEGVRVGDQFSTVRSRINADYNITNWLS
                     VGMNTQFSDRNENSVPASLDFYVNSPYGEMWDANGNLKRYPHGHSDNPLLAYYRTDRL
                     NKTNSIFSNLFANIRLPYGFNFRVSFQPRYGTTKYGSFTNISEKLGGLSTETPSGERR
                     ESSTMNWMVDNLLTWKREIGIHNFDLTLLANIEENQYWSTSMTNKNFKPNQNLSYHGL
                     HFGDSPGISINDTRSTGDALMARLNYTLLNRYLLTASVRRDGYSAFGQKNPRAVFPAF
                     ALAWVVSDESFFNIGLIDRMKFRVSWGANGNRDIGIYSALANTASSLWFDGTSTRVGI
                     YNSTLANTGLRWERTTSLNFGLDITLMKGRLDLSADYYDMTTTDLLMRRVLPQVTGFT
                     SIMANLGELGNKGFEMTLNSINVSQRDFTWKSNLVFSLNRNKIKKLFGDIGTYTLLNE
                     QREGELPDFTNLWFPGEALDVVWDYDIIGIYQLNEADAAAVYGLQPGDFKAVDVNNDG
                     KFVNVQDKKFIGYNSPRYRLGIRNDFNFMKNFTASVFIRSDLGHIGSYSPALNSGYES
                     NDRWNRNVGPVPYWTADRPNNEYARLNCIRAVFGGGLMIYKPRSFTRIQDISLTYNLP
                     ADLAKRISLNNLQFFGSIRNLATFTKWPGWDPESGMTPMPRTYTIGLNLSL"
     gene            3109..4881
                     /locus_tag="PLZ75_02770"
     CDS             3109..4881
                     /locus_tag="PLZ75_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS71354.1"
                     /translation="MKRIKITSLITLFFLLCISCSDEWLKPDPLSFFAPENIYVDAAG
                     FEAGLVRCRKEMNAENHGYQNPIAVDVMYTDIAVPLRQSDFTQNTPSLVQQVPLVLTI
                     FSSFYGYIKNANTIISRIDNITWTDEGLRNRILSEALWFRAYWYYRLVHTYGDIPWVG
                     EELTGAKLDYYSTSRWAILGKLQEDLEYAVKWLPITSGVPGNVTKGAANHLLAKIYLA
                     NCEFDKAVSATTEVINGPYALMKERFGVDANKKWHNVMWDLHRYQNKNISANKETIYA
                     TVDRPEAPTETWWSNPGTYSMRLYTPSYWKVMDATGNRACNWQTVAGDTLGIGNGDVR
                     TNDFWHYWMWNEAGYTWENTPDMRRAPNNWIEMGKETAEIITVRPESPNLGEPLSKQY
                     YKSLDDTVDTWYSWPQYKTYTPTPNYHQPHGGQGDWYTFRLAETYLLRAEAYYWKGQP
                     DLAASDINVVRDRAHAPLITSDDVSIDYIFTERAKELYTEEPRHSEMVRVAFIFGKLN
                     RDGYSLSTMTEKNWYFDRIMKYNPFYKEPRYRFWDATAYMEPKHFLWPIPQTVITANT
                     MGVINQNYGYDGYENNVLPLETIP"
     gene            4977..7385
                     /locus_tag="PLZ75_02775"
     CDS             4977..7385
                     /locus_tag="PLZ75_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOS71355.1"
                     /translation="MRKHLIGSKSFKLLTVIILIFCSCKNQQPAESFLTDIIPQVSNT
                     AKQKAELEKLLAWLPPDRTINGRVSFLDETFRDWLARTGELPPEFDKMPSLPFLPDPL
                     ILDEGGRNIPVKTKKQWREKREWMKRQLEYYITGTYPPPPENMTSVIVSENMDNETIL
                     QIVELTFGPDNRAKLTLELMIPPGKGPFPVFLTQWNHREWAQIAVRRGYIGCVYAGAD
                     SKDDTEEYSEIWAGRYDFTRLMRRAFGASRAIDYLYTLPFVDRDKIGITGHSRNGKLS
                     LIAAAFDERIGACIPSSGGTGAEVPWRFCSQNYDVEDIALLACAQPAWLHPRLRFFTG
                     RENKLPVDQNLFMALIAPRGLMLSTAITEGASNPWGIEQAYHATGKVYEFLGAPDNLA
                     IRSRYGLHSICARDMEDYMDFFDYVFKRSNHKPENRLFYNYSFEDWRRLSGEKVNPLD
                     FPVKGIGDLLADAGQKSITTVSEWESEREEIRKAIQRVLGDEPPGVTNPGPGSLSMGG
                     RGESTFGSFLQRPGPAAGMGVMAITPYNGFGDQLFGYLYYPIDKNGKPENNNLPVVIY
                     LHEYDYSKGFNSYHQIESFFRSVCERGYAVFAFDMIGFGNRIEEGTRFYHRYPRWSRM
                     GKMIADVRGAVDALTNLDFTDSSRIYVAGYSLGATVGLHAAALDERIAGVVSVAGFTP
                     MRLNTAERGTGGIKAYSHLHGLLPRLGYFAGNESRIPYDYHEVLAAIAPRPVLVIAPL
                     LDKDAHWEDVQECVKQAGKVYSLYGAAGNIELFSPDDYSRFSVEMRDSTYSWLQKRQL
                     SPGP"
     gene            complement(7481..8104)
                     /locus_tag="PLZ75_02780"
     CDS             complement(7481..8104)
                     /locus_tag="PLZ75_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOS71356.1"
                     /translation="MKHREKKLAQVFIFALLVSFTACQTQAPVKEKDDSIRGSIAVLV
                     AEGFHDGEAYMPMGYLVNNGYEITVIGPERGKVKAYNSEFTINIDKSVSEVSPDDFDA
                     LILPGGRGPSVLRENDRVIEFVKAFWNTGKVTAAICHGPQVLVTADLLKGLVLTGTGA
                     IKGEIEQAGATYVDSTVVVYGNLITSRNPQDLKNFSSSVVEALEKQN"
     gene            complement(8175..8247)
                     /locus_tag="PLZ75_02785"
     tRNA            complement(8175..8247)
                     /locus_tag="PLZ75_02785"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8212..8214),aa:Gln,seq:ctg)
     gene            complement(8892..10607)
                     /locus_tag="PLZ75_02790"
     CDS             complement(8892..10607)
                     /locus_tag="PLZ75_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOS71357.1"
                     /translation="MNGEKTKEAGESKVPVSFIEQIIIDDLREGKNNGRIHTRFPPEP
                     NGYLHIGHAKAICLDFGMAEKYGGRCNLRFDDTNPVKEEVEYIDSIKEDIHWLGFDWD
                     NREYYASDYFGKLFDFAVDLIKKGLAYVDDQPSDIIAEQKGTPTQAGTESPFIRRSVQ
                     ENLDLFYRMNEGEFEEGSRVLRARIDMSSPNMHMRDPIIYRIIKTPHHRTGNSWNVYP
                     MYDFAHGQCDYFEGITHSLCTLEFEIHRPLYDWFIDQLMTSAYRPRQIEFNRLNLTYT
                     VMSKRRLLELVRDGKVKGWDDPRMPTLCGLRRRGYTPESVRKFIDLIGYTKVEAINDV
                     SLLEHAVREDLNLKAPRVFGVLDPLRVVITNYPADKVEEMEIINNPEDESMGSRMVPF
                     SKEIYIERDDFMENPPHKFFRLAPGSEVRLKGAYIIRCESFKKNPESGIIEEVYCTYD
                     PETKSGKHLSAKKVKGTLHWVSAQHAIDAEVRLYDRLFSHEDPSGQKDRDYREFLNPD
                     SLKVLKNCKLEPSLGSARPYDKFQFQRLGYFCTDPDSTPARPVFNRTVSLRDTWARMV
                     SKKQE"
     gene            complement(10679..10972)
                     /locus_tag="PLZ75_02795"
     CDS             complement(10679..10972)
                     /locus_tag="PLZ75_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOS71358.1"
                     /translation="MKRYKDLDPLLHSQLRLAVMSILISVETADFTFIREQTGATAGN
                     LSIQIGKLKEAGYIEVTKKFRDNYPQTKCRITELGRERFNEYVSALKDYLNQR"
     gene            complement(10959..11546)
                     /locus_tag="PLZ75_02800"
     CDS             complement(10959..11546)
                     /locus_tag="PLZ75_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71359.1"
                     /translation="MENGEKIMTGEESFRLITEMINRTKVNIRNSSFHYLFWGWLILA
                     CSLAEYILWKFIGAGSHWYVWFLSIPGVFVSMIYGYRTGKKERFHTYADNIYVWTWMA
                     FLIAAAVLFIISSGRMQFVAPLILTLAAVPTFLSGFIIKFRPLIFGGITFWIFALIAR
                     FAGNEISALTVPVAMLTGYLLPGYMLKRKVDHEKV"
     gene            11694..12044
                     /gene="folB"
                     /locus_tag="PLZ75_02805"
     CDS             11694..12044
                     /gene="folB"
                     /locus_tag="PLZ75_02805"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOS71360.1"
                     /translation="MGLIQIEGMEFYAFHGHFREEQIVGSRFLVDLTIETDLEKPAES
                     DNLRDAVNYQEAYKLIKVQMEQKSHLLENIASRILDSLYSEMTGIRKATVKVSKMNPP
                     VGGKVNCVSIIMER"
     gene            12248..13804
                     /locus_tag="PLZ75_02810"
     CDS             12248..13804
                     /locus_tag="PLZ75_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOS71361.1"
                     /translation="MNLRKYLLFFLLAAFVSLNAGAQVITSKEIDVLVENVMKTFDVP
                     GIAVGIVKDGKVIHSKGYGVRSLATGQKVDSNTLFGIASNSKAFTAAALGILVDENKL
                     YWNDRVIDYIPEFRLYDPYVTSEFTIADLLTHRSGLGLGAGDLMIWPGRNDFTVNDII
                     HNLRFLKPVSSFRSKYDYDNLLYIVAGEIVARVSGMSWAEFIEKRIMEPLEMKGSAAS
                     FSRLKDKSNVIDAHAPVEGKVQVIDRSFNEMAVAAGGIYSNISDMSKWVIMQLNRGRY
                     GGNPGRKLFSENVHRTMWSPHTIIPVAESAEYRIHFSAYGLGWRLNDIMGYKQVGHTG
                     GLAGIVTQVTLIPGLNLGIMVFTNQQAGAAFRAITNTILDSYFGIRGADRVKEYYENE
                     KRSIENAKRISDDIWKNIEARESPAFKPDNSAYTGTYNDNWFGEVTISDRNGTLWFEA
                     KRSPVLSGAMLPYKGNTFVVKWNDRSLDADAFAVFTLNVEGRASGMTMKAISPLTDFS
                     YDFHDLDFRR"
     gene            complement(13870..14535)
                     /locus_tag="PLZ75_02815"
     CDS             complement(13870..14535)
                     /locus_tag="PLZ75_02815"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOS71362.1"
                     /translation="MDKIKIGIFIRIKEESQKSNTIGMPDNIFNLNNPDFFGILLRFV
                     INTLFHIVLIRVVYFRYTKKEKFLFTFFLLGITVFFITSMLKSVFIEFGMAVGLFAIF
                     SILRFRTRNFSLKDMSYMFATIGLSVINSLKLVGFPLLGVLIFNIILIVCVIILEEFT
                     LQYNTTTYRIIIRDLELLKTGKKQKILKEISTLTGKDILRYRIREISYKDMIAKIDVT
                     YKD"
     gene            complement(14554..15486)
                     /locus_tag="PLZ75_02820"
     CDS             complement(14554..15486)
                     /locus_tag="PLZ75_02820"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HOS71363.1"
                     /translation="MKQLLLIFLIIYPLMTDAQTGNKSAEGRSDPGYDLSAPDKVYIL
                     PPVLLEISGIAVLDGSTIACVEDEHEAVYIYDISKNQITRQIFSGHKGDYEGIARVGR
                     TIYLLRSDGMLTEIADYDKDKFKRTIYKTNIPWKDNEGLCHDQKNNRLLVAPKEVPKK
                     DSPCSDFRLIYSFDLTDKKMQSEPVFRFSMAEIRKYALDNKIRVPVKSKKGTVSPVPD
                     IHFRTSAIGIHPVTGNLFVISGPESILFVFDRKGNIVFMEQLDKNLFRQPEGISFMKN
                     GDMFISNEGKGKAATIVKFSFRGDDREIYQHISR"
BASE COUNT         4599 a         3229 c         3598 g         4448 t
ORIGIN      
        1 gataaattat tttattctat tctgcattct ggttagtttc cctcttattg gttatggcca
       61 atttgaagta agaggaaagg tcacagattc tcaatcagat caaccactcg caggagttac
      121 ggttgttata aaaggttcta ctactggtac gcttacggat attgatggta attattcaat
      181 tttagctccc agtgagaaaa gcattcttgc attttctttt atcggatata tttcgcggga
      241 agttacagta ggtaatcaaa aggagataga cattgcactt gaaacagatg ttttaagact
      301 tgaagaagta gtggttattg gttacggtac agcaaagaaa agtgatctta ccggatcagt
      361 tgtaagtgta aaagccgaaa actacaaaac acagcaaatg gtacaattca ctgacatgct
      421 gacaggtacg atagcaggtt ttaatgcgaa tcagggaact tcagcatcag gaggcggagc
      481 ctcacttgaa ataagggggc caaaatctct ttctgcaggc acagacccac ttatcgtcct
      541 tgatggtgtc atatatactg ggacactccg tgagataaat cccaatgata ttgcttcggt
      601 agatattctt aaagatgcca gctctgctgc ggtatttggt gccaaagcag cagcaggagt
      661 tgttttgatt accactaccc ggggaaggct tggaaagcct acaattaatt tctcaaccaa
      721 gatcggcatt gctgaagtaa ataataaacg ccgggggctt ggacctgaag aatatatacg
      781 ttttcgtcag gattatttca gacagatgtt ccccagcatt gattataatt tttatactca
      841 tcctgacaaa cttccgcctg gtgtgtcaat tgaccaatgg cgggctttaa gctcttctcc
      901 tgtagatgat aacatccagg aatggatggg gagaatgagg ttttatccgg aggaacagga
      961 aaactatcgt gaaggcagaa ccatggatat gtttgattat gtattcagga aaggtcttca
     1021 gcaggactac gatcttagta tttcaggagg aactgaaaag gcaacatact attggtcaat
     1081 aggatatgcc aataatgagg gagtcagagt cggagaccag ttctcaacag tccgttcccg
     1141 aatcaatgct gattataata taacaaattg gttgtcagtt gggatgaata cccaattttc
     1201 tgacaggaat gaaaattctg ttcccgcaag cctcgatttt tatgtcaaca gcccctatgg
     1261 tgaaatgtgg gatgcaaatg gaaatttaaa aagatatcct cacggtcatt cggataatcc
     1321 tttgctggcc tactacagga cagacagatt aaataaaacc aacagtattt tttcaaattt
     1381 atttgcgaac atcagattac catatggatt taatttcaga gtatcctttc agccgcgtta
     1441 tggtaccaca aaatatggga gtttcacgaa cataagtgaa aaactgggtg gtctgtcaac
     1501 agaaacacct tcaggagaaa gaagagagtc ctcaactatg aattggatgg tagataatct
     1561 tttaacctgg aaaagagaaa taggtataca taattttgat ttgactttat tggccaatat
     1621 agaagaaaac cagtattggt caacgtcaat gacaaacaag aattttaagc cgaatcagaa
     1681 tctgtcttac cacggactcc attttggtga ttcaccagga ataagtatca atgatacaag
     1741 aagtaccggt gatgcattga tggcacgtct aaattataca ttattaaacc gttatttgtt
     1801 aactgcatcc gtaaggagag atggttattc tgcttttgga cagaaaaatc ccagggctgt
     1861 gtttccggcc tttgcacttg catgggtagt ttcggacgaa agtttcttca atatcggatt
     1921 aattgacagg atgaagttta gagtttcctg gggtgccaat ggcaacagag acattggtat
     1981 ttattccgca ctggctaata cagcttccag cttatggttc gatggtacat caacgcgtgt
     2041 tggtatctat aattcaactc ttgcaaatac aggtttgaga tgggaaagaa ccacctcact
     2101 aaatttcgga ctggatatta ctttgatgaa aggcagactg gatttgtcgg ccgattatta
     2161 tgacatgacc actacagacc ttttaatgag acgtgttctg ccccaggtta caggcttcac
     2221 cagtataatg gcaaacctgg gtgagcttgg aaataaaggt tttgaaatga cactgaattc
     2281 aataaatgtc agccaaagag actttacctg gaaatctaat ctggttttct ctctgaatag
     2341 aaataaaata aagaaattat ttggagatat aggaacctat accctgctta atgagcaaag
     2401 agagggagaa cttccggatt ttactaatct atggtttccg ggtgaggctc ttgatgttgt
     2461 ctgggattac gatataatag gtatttatca actgaatgaa gcggatgcag cagctgtcta
     2521 tggcctccag ccaggagatt tcaaagccgt tgatgtcaac aatgacggta agtttgtaaa
     2581 tgtacaggac aagaaattta taggatataa tagcccgaga tacaggttgg gaataagaaa
     2641 tgatttcaac tttatgaaga atttcactgc ctcagttttt attcgttcag acctcggaca
     2701 tattggttca tacagtcctg ctctgaacag tggctatgaa tcaaacgaca ggtggaacag
     2761 aaatgtcgga ccggtacctt actggacagc tgacagacct aataatgaat acgcacgttt
     2821 gaactgcatc cgtgccgtgt ttggaggagg actgatgatt tacaagccca gatctttcac
     2881 aagaatacag gatatttcac tgacatacaa tcttcctgct gatctagcca aaagaatcag
     2941 tctgaataat ctgcagtttt ttggatcaat aagaaatctt gcaacattta ctaaatggcc
     3001 aggttgggat cctgagtcag gaatgactcc tatgccgaga acctatacaa ttggcctcaa
     3061 tctgtcatta taatttatgt tgaatgtaac tctaaacaga taaataaaat gaaacgaata
     3121 aaaataacat cccttattac actcttcttt ctattgtgta tttcctgttc ggatgaatgg
     3181 ttaaaacctg acccgctttc tttctttgct cccgaaaata tttatgtgga tgctgccgga
     3241 tttgaagcag gacttgtaag atgcagaaaa gaaatgaatg cggaaaacca cggttatcag
     3301 aatccgattg cggttgatgt catgtataca gatattgcag tacctcttag acaaagtgat
     3361 ttcacacaga atacacccag ccttgttcag caggtaccac tagtattgac aatattcagt
     3421 agtttttacg ggtatattaa aaatgcaaat actattattt cacgaattga taacattact
     3481 tggacagacg aagggctaag aaacagaata ttgtcggaag ctttgtggtt tcgtgcttac
     3541 tggtattatc gtctggtaca tacctatgga gatattccat gggttggaga ggaattaact
     3601 ggagcaaaac ttgattatta ttctacatca aggtgggcaa ttctgggaaa acttcaggag
     3661 gatctggaat acgcagtaaa gtggcttccg attacttctg gagttcccgg taatgttaca
     3721 aaaggtgcgg caaatcatct tttggcaaaa atatatttgg ccaattgcga atttgataaa
     3781 gctgtaagcg caacaactga agtcattaat ggtccatatg ctctgatgaa agaaagattt
     3841 ggagttgacg cgaataagaa atggcataat gtgatgtggg atttacaccg ttatcagaat
     3901 aaaaatatat ctgccaataa agaaacgatt tatgcaacag ttgacagacc agaagctccc
     3961 acagaaacat ggtggagtaa ccctggcacg tattcaatga gactttatac gccctcatac
     4021 tggaaagtaa tggatgcaac gggtaatcga gcttgcaact ggcaaacagt ggcgggtgat
     4081 actcttggta ttggcaatgg agatgtgaga accaatgact tctggcatta ctggatgtgg
     4141 aatgaggctg gttatacctg ggagaatact ccggatatga gaagagctcc caataattgg
     4201 attgaaatgg gtaaggaaac agcagaaatt attactgtac gacccgagtc tccgaatttg
     4261 ggagaacctt tatcaaaaca atattataaa agtctggatg atactgtgga cacctggtat
     4321 tcatggccac aatataaaac atatactcct acaccaaatt atcaccagcc gcatggggga
     4381 caaggagatt ggtatacatt ccggctcgct gaaacatatc ttctcagggc agaagcttat
     4441 tactggaaag ggcaacccga tttagcagca tcagacataa atgtggttag agaccgtgct
     4501 catgcaccac ttattacatc agatgacgtt tctatcgatt atatatttac agaacgcgcc
     4561 aaggaattat atacggaaga gccacgtcat tcagaaatgg tacgggttgc attcattttt
     4621 gggaagctaa acagggatgg ttacagttta tcaacaatga cagagaagaa ttggtatttt
     4681 gacaggatta tgaaatataa tccgttttat aaagaaccca ggtacagatt ctgggatgcc
     4741 acggcataca tggaaccaaa gcattttctg tggcccattc ctcagactgt cattactgct
     4801 aacaccatgg gtgtgatcaa tcagaattac gggtatgacg gctatgaaaa taatgtcctt
     4861 ccgctcgaaa cgatacctta aattatttat ttaaatgtgt ggaaaaagga aattgattcc
     4921 acacatttat tttcattacc ggcattttta taatcaaaac tttgtttaaa attattatgc
     4981 gtaaacatct gattggttct aagagcttta aattattaac tgtaataatt ctaatctttt
     5041 gttcttgtaa gaatcaacaa cctgcggaat cattcctgac ggatatcatt ccgcaggttt
     5101 ctaatacggc aaaacagaag gctgaactgg aaaagcttct tgcctggctt cctcccgacc
     5161 ggactataaa cggacgggtt tcattcctgg atgaaacatt ccgcgactgg ctggccagaa
     5221 caggtgaact tcctcctgaa tttgataaaa tgccttcact gcctttcctt cccgatccgc
     5281 tgatactgga tgaaggcggc aggaacatac ctgttaaaac aaagaaacag tggagagaaa
     5341 agcgggaatg gatgaaaagg cagctggaat attatattac cggtacttac ccgcctccgc
     5401 ctgaaaacat gacatcagta attgtgagtg aaaatatgga taatgaaaca attttacaga
     5461 ttgttgagct cacattcgga ccggacaaca gggcgaaact gacacttgag ctgatgatac
     5521 caccggggaa agggcctttc cccgtattcc ttacccagtg gaatcatcgt gaatgggcac
     5581 agatagctgt cagaaggggc tatatcggat gtgtttatgc aggagcagat tcaaaagatg
     5641 atacggagga atattcggag atatgggcag gccgctatga tttcacaaga ctgatgcgca
     5701 gagccttcgg agcttccagg gcaatagact atctgtacac ccttcctttt gtggacaggg
     5761 ataaaatcgg aatcacaggc cattccagaa atggaaaatt atctcttatt gcggctgcat
     5821 ttgatgaaag aattggtgcc tgcataccca gtagcggggg aaccggtgcc gaggttccct
     5881 ggcgattctg ttcacagaat tatgatgttg aggatattgc actgctggca tgtgctcagc
     5941 ctgcatggct tcatccgcgg ctcaggttct tcaccggccg tgaaaacaaa ctgcctgttg
     6001 accagaatct ttttatggcc cttatcgcac ccaggggact gatgttaagc acggcaataa
     6061 cggaaggggc aagcaatccc tggggtattg agcaggccta tcatgccacc gggaaagtat
     6121 atgagtttct gggtgccccc gataacctgg ccatcaggtc acgttacggc ctgcacagta
     6181 tttgtgcccg ggatatggaa gactatatgg atttctttga ttacgttttt aaacgcagca
     6241 atcataaacc ggagaatcgc ctgttttaca actattcatt cgaagactgg cgcaggctga
     6301 gcggagaaaa ggtcaatccc ctggactttc cggttaaggg tatcggggat ttgcttgcag
     6361 atgccgggca gaaatcaatc accactgtca gtgaatggga atcagagagg gaagagataa
     6421 gaaaagccat tcaacgggtt ctgggggatg aacctccggg cgttacaaat ccgggccctg
     6481 gaagtttaag catgggaggc agaggcgaaa gtactttcgg ttcatttctg cagcggccgg
     6541 gcccggcagc aggaatgggg gtaatggcta taacacccta caatggtttc ggagatcagc
     6601 tgttcggcta tctttactat cctatagata aaaacgggaa acctgaaaac aataatcttc
     6661 ctgttgttat atatcttcat gaatatgatt attcaaaagg attcaattcc tatcatcaga
     6721 ttgaatcatt tttcagatca gtatgcgaaa gaggatatgc cgtattcgcc ttcgatatga
     6781 taggtttcgg aaaccgcatt gaagagggga cacgctttta tcacaggtat ccccgctggt
     6841 ccagaatggg caagatgata gctgatgtaa gaggagctgt tgatgcactg acaaatctgg
     6901 actttacaga cagttcacga atatatgttg ccggttattc acttggagca acagtcggcc
     6961 tgcatgctgc ggccctggat gaacgtattg cgggagtggt atccgttgcc ggattcacgc
     7021 caatgcgcct gaacacagct gaaaggggaa ccggaggaat aaaggcatat tcacatctgc
     7081 acggactgtt gccaagactg ggatatttcg ccggcaacga atcccggata ccctatgact
     7141 accacgaagt actggcggca atagcaccga ggcctgttct ggttatagca cctttgctgg
     7201 ataaggatgc ccattgggaa gatgtacagg aatgcgttaa gcaggcaggg aaagtatatt
     7261 ctctctatgg tgctgccggt aatattgagt tgttttcacc tgatgactac agcagatttt
     7321 cagtggaaat gcgtgacagt acatattcct ggctgcagaa gagacaactc tcccccggtc
     7381 catgaattgt tttagcttat aaatctgcca ggtatccttg ccaggaatgc agcaaagggt
     7441 gaaaatctgc caatccggat aaataaacca gaatgttacc ttagttttgc ttttccaggg
     7501 cttcaacaac agatgaggaa aagtttttaa gatcctgcgg gtttcttgag gtaatcagat
     7561 tcccgtatac aactaccgtt gaatcaacat atgttgctcc tgcctgttcg atttcgcctt
     7621 taatggcacc agtgcctgtc agaacaagac ctttcagcaa atcagccgta acaagcacct
     7681 gggggccatg gcagattgca gcggttactt ttccggtatt ccagaaagcc tttacaaatt
     7741 caataaccct gtcattttct ctcaataccg aaggtcctct tcctcccgga aggatcagag
     7801 catcaaaatc atccggactg acctctgata ccgacttgtc aatatttatt gtgaattcac
     7861 tgttgtatgc ctttaccttt ccgcgttcag gtccgattac agtaatttca taaccattgt
     7921 taaccagata tcccatgggc atataggctt ccccgtcatg gaatccttct gcaaccaata
     7981 cagcaatact tcctcgtatt gaatcatctt tttccttaac tggtgcctgt gtctggcatg
     8041 cagtaaaaga aaccagtaaa gcaaaaatga aaacctgtgc taatttcttt tctctgtgtt
     8101 tcatgatact ccttgtgttt ggttaattaa attccggttt aggtaaaaaa tcccgccggg
     8161 gcgggatgtt tttgttgctc cgccagggct cgaacctgga cttttctgat ccagagtcag
     8221 acgtgttgcc aattacacca cggagcagta tttgggaatg caaatatata aaatcctgat
     8281 aaaccttcat gatataattt taaaatgacc gggaaaaaga tctgttgtca tgaagttttc
     8341 cggaaaaagt ccttaataaa tgtattctga aaataattcc cctgaaggaa cagaccggta
     8401 atatattgtg ccccggttga aagacgaagc tgtttctcat ctgtaattca ggaaattgcg
     8461 cagggaaaca agcgaaggaa tacaaaggaa aatgtgaagg tacatacaag taaacatgtt
     8521 ggaacaagca gataagctgc gggtaacata aagggaaaca ctttgaacct tatttaaaag
     8581 agcgagggcg ctccacactg taacgccctc tgagaaaatt gccggaatac tccggcaatc
     8641 ggatcatagt atgaaataga taacacttat acgaaaagcc tggaaaaaag ttataccggt
     8701 cctgtacagg tctttccggc tcctttcagc gtgggaaacg gcacctcatt gcgggaactg
     8761 gcacctcatt gcgggaatga tacctcattg cgggaacgat acctcattgg ggtaaccggc
     8821 acctcatttt ctggacaggt tttcagcggt agatgtgact ggcggagctg aggatcctgc
     8881 ttcccctgcc cttattcctg tttcttgctg accatcctgg cccaggtatc cctgagggag
     8941 actgtcctgt tgaaaaccgg gcgggcaggt gtggaatccg ggtcggtaca aaaatatccc
     9001 agccgctgga actggaattt atcgtatggc cgtgctgaac cgagagatgg ttccagttta
     9061 cagtttttaa ggactttaag ggaatcagga ttgagaaact ccctgtaatc cctgtctttc
     9121 tggcccgaag gatcttcatg actgaagaga cggtcataca gtcttacctc cgcatcaatg
     9181 gcatgttgtg cagaaaccca gtgaagggtg cctttgacct ttttcgcgga taaatgtttt
     9241 ccgctttttg tctcaggatc ataggtacag tacacttctt caatgattcc gctttcagga
     9301 ttttttttga aggattcgca tcttatgatg tatgcacctt tcaggcggac ttccgatccg
     9361 ggagccaggc ggaaaaattt atgaggaggg ttttccatga aatcgtcacg ttcaatatag
     9421 atctccttac tgaaggggac catccttgat cccatggatt catcctcggg attgttgatt
     9481 atttccattt cctcgacctt gtccgcagga tagttggtga taaccaccct gagaggatcc
     9541 agcaccccga aaaccctggg agctttcaga ttgagatcct cgcgcacggc atgctcaagg
     9601 agtgaaacat catttatagc ctcaaccttt gtatatccta tgagatcaat gaatttccgc
     9661 actgattccg gggtatagcc ccgtcttcga aggccgcaga gagtgggcat ccggggatca
     9721 tcccagcctt tgacctttcc gtcgcgtaca agctccagga gtctcctttt gctcataaca
     9781 gtatatgtca ggttcaggcg gttaaattca atctgccttg gcctgtaagc ggaggtcata
     9841 agctgatcaa tgaaccagtc gtacaggggg cggtgtatct caaactccag ggtacagagg
     9901 gaatgtgtga ttccttcaaa ataatcacac tgtccgtgtg cgaaatcata catcggataa
     9961 acattccagc tgttgcccgt gcggtgatgc ggagtcttaa tgatcctgta aataatagga
    10021 tcgcgcatgt gcatgttggg tgatgacatg tctatccggg cacggaggac acggctgcct
    10081 tcctcaaatt cgccttcatt catcctgtaa aacagatcca ggttctcctg aacggatctc
    10141 ctgatgaagg ggctttctgt accggcctga gtgggagtgc ccttttgttc cgcaataata
    10201 tccgatggct ggtcatccac gtatgccagg ccttttttta tgagatcaac ggcaaagtcg
    10261 aaaagttttc cgaaataatc cgatgcataa tattcgcggt tatcccagtc aaagcccagc
    10321 cagtgaatgt cttcctttat cgaatcgata tattcaactt cctcttttac aggattggtg
    10381 tcatcaaatc tcagattgca tctgcctccg tatttttcgg ccattccgaa atcaaggcag
    10441 atggccttgg catgaccgat gtgaaggtag ccgttaggct cgggagggaa cctggtgtgt
    10501 attcttccgt tgttctttcc ttccctgaga tcatcaatga tgatttgttc aataaagctg
    10561 accggtactt ttgattctcc ggcctctttt gttttctctc cgttcatcct gaaaaattaa
    10621 gattacaaaa ataggatgaa tttcagggaa aaaaaacatg gggcaaagaa ccaatatcct
    10681 atctctgatt caggtaatct ttcagggcgc tgacatattc attgaatctt tcccttccaa
    10741 gttcggtaat cctgcacttt gtctgagggt aattatccct gaactttttt gtcacctcaa
    10801 tgtatcccgc ctctttcagc ttgcctatct gaatactcag gttcccggct gtggcccctg
    10861 tctgttcccg gatgaaggtg aaatccgcgg tttcaacact gatgaggatt gacatcactg
    10921 caagcctcag ctgtgaatgt aacaggggat caagatcctt ataccttttc atgatcaacc
    10981 tttcttttta gcatataccc cggtaaaaga tagccggtaa gcatggcaac cggcactgta
    11041 agggccgata tctcattccc cgcgaaacgg gcaatcaggg caaatatcca gaaagtaatc
    11101 cccccgaata tcagaggcct gaatttaata atgaaccctg acagaaaggt tggtacggct
    11161 gccagtgtca gtatgagcgg agcaacgaac tgcatcctcc ccgatgatat gatgaacagg
    11221 acagcagcag ctatcaggaa ggccatccag gtccagacgt aaatattgtc ggcataggta
    11281 tggaatcttt ctttcttccc tgttctgtaa ccgtatatca tggaaacaaa aacccccggt
    11341 atcgacagaa accacacgta ccagtgagat ccggcgccaa tgaatttcca cagaatatat
    11401 tccgccaggc tgcatgcaag tatgagccag ccccagaaaa gatagtgaaa actgctgttt
    11461 ctgatattca cttttgtcct gttgatcatc tcggtaatca gcctgaagct ctcttcacct
    11521 gtcattattt tttctccgtt ttccataatt attcttattt ttaattgtga atggaattgt
    11581 ttataaatat attgtaaata taaagtagtt tataatataa accaaatatt ccggcaggaa
    11641 ttttattctg cattctaaaa aaatattatt attcggacta tcaatacact aatatgggtc
    11701 taattcagat tgaaggtatg gaattttatg cctttcacgg gcatttcagg gaagaacaga
    11761 tagttggcag caggtttctg gtggatctca ccattgaaac tgatcttgaa aaaccggcag
    11821 agagcgacaa tctccgggat gcggtcaatt atcaggaggc ttacaagtta atcaaggttc
    11881 agatggaaca gaaatcacat ttgcttgaaa acattgcttc aaggatactc gattcgctct
    11941 attcggaaat gaccggaatc aggaaggcta ctgtgaaggt ctcgaaaatg aatcccccgg
    12001 tcggcggcaa ggtaaattgt gtcagcatta taatggaaag atagtatttt agcggtagag
    12061 gaattttgaa tcattcttta gtcgggccgt attatgccgg aaatctgctg aaaaccatca
    12121 ggtattcggg aggaactatt caggttctgt attaaccggg atgaaattcc ggtataattc
    12181 aggggattcc ggcgaaaaca ggtcactgac tttttcagat aattatgaat catttaaata
    12241 gtataaaatg aatttgagaa agtatttgct tttttttctc ctggcggcat ttgtcagcct
    12301 caatgcagga gctcaggtaa tcacttccaa agagattgac gttctggttg agaatgttat
    12361 gaaaaccttt gacgtaccag gcattgccgt tggcattgta aaggatggaa aggtaattca
    12421 ttcaaaagga tatggtgtca gatcgcttgc aacgggacag aaagttgatt caaataccct
    12481 gttcggcatt gcttcaaaca gcaaggcatt tactgcggcg gcactcggca ttctggtgga
    12541 tgaaaataag ctttactgga acgacagggt tattgattat attcctgaat tccggcttta
    12601 tgatccctat gttacaagtg aattcaccat agccgatctg ctgactcaca gaagcggcct
    12661 gggtctggga gcgggggacc tcatgatctg gccgggaagg aatgatttca cggtaaatga
    12721 tatcattcac aatctcaggt ttcttaaacc ggtttcaagt ttcaggtcga aatatgatta
    12781 tgacaatctt ctttatatag ttgcgggaga gattgtggcc agggtttcag ggatgagctg
    12841 ggcagagttt attgagaaac ggattatgga acctcttgaa atgaagggaa gtgctgcctc
    12901 attcagccgg ctgaaagaca aatcgaacgt gatcgatgcg cacgctcccg tagaggggaa
    12961 ggtacaggtg attgacagga gtttcaatga aatggctgta gcagccggag gtatttacag
    13021 taatatctcc gacatgagca aatgggttat tatgcagctg aacaggggca gatacggggg
    13081 gaatcccggc aggaagcttt tcagtgagaa tgtccaccgg acaatgtggt cgcctcatac
    13141 aataatcccg gttgcggaat cagctgaata cagaatacat ttttcagcat acggtctggg
    13201 atggagactc aatgacataa tgggatacaa gcaggtgggc cataccggag ggctggctgg
    13261 catcgtgacc caggtaaccc ttattcccgg actgaacctg ggaatcatgg tctttaccaa
    13321 tcagcaggca ggagcagctt tccgtgccat aacaaacacg attcttgaca gttattttgg
    13381 catcagggga gctgacaggg taaaagagta ttatgaaaac gaaaaaagga gtattgagaa
    13441 tgcgaaaagg ataagcgatg atatatggaa aaatatagaa gcccgggaat caccggcatt
    13501 taaaccggat aattcggctt acaccggtac atacaacgat aactggttcg gcgaggtaac
    13561 aatatcggac aggaacggaa ctctctggtt tgaggcgaaa agatctcctg tcctcagcgg
    13621 ggcaatgctt ccctacaagg gcaacacctt tgtggtgaaa tggaacgaca ggagcctgga
    13681 tgcggatgcc tttgcggttt ttaccctcaa tgtggagggc agggcttccg gaatgaccat
    13741 gaaggcaata tctcccctca cggacttcag ttatgatttt catgaccttg acttcaggag
    13801 atgaactata tcctgaagtc aatgatcagg attaacgccg gctccggcag gcggaatgaa
    13861 cagcggcagt tagtccttgt aagtgacgtc tattttcgca atcatgtcct tgtaggatat
    13921 ttcccggatc ctgtatctga gtatatcctt ccccgttaaa gtcgagattt ccttcaggat
    13981 tttctgcttt ttacctgttt taagcaattc aaggtccctg atgattatcc tgtaggttgt
    14041 agtgttgtat tgaagggtga attcttcgag gataatcacg cacacaatca ggataatgtt
    14101 gaatattaaa accccgagca gcgggaatcc caccagttta agggaattaa tcacggataa
    14161 tcctatggtt gcaaacatat aggacatatc cttcaggctg aaatttctgg ttctgaatct
    14221 cagtatggag aaaattgcaa agagaccgac cgccattccg aattcaataa agaccgattt
    14281 cagcattgag gtgatgaaaa ataccgttat gccaagcagg aagaatgtaa aaagaaattt
    14341 ctccttcttg gtataccgga aatataccac cctgattaga acaatatgga acaacgtatt
    14401 tattacaaag cgcagcagta tcccgaaaaa atcaggattg ttcagattga aaatattgtc
    14461 aggcatgccg atagtattgg atttttgact ttcttcctta atgcggatga aaatacctat
    14521 ttttatctta tccaatacag ctaattatca acatcatcgg ctgatgtgtt gataaatttc
    14581 ccggtcatcc cccctgaaac tgaatttaac gattgtggct gccttgcctt ttccttcatt
    14641 ggaaatgaac atatctccgt ttttcatgaa gcttattcct tccggctgcc tgaacagatt
    14701 tttgtcaagc tgttccatga atacaatatt cccctttctg tcaaagacaa agagaatact
    14761 ttccggacct gaaatgacaa acaagttccc cgtcaccggg tgaattccta ttgcagatgt
    14821 tctgaaatgt atgtcaggga cgggactgac ggttcccttc ttacttttca caggcaccct
    14881 gattttattg tccagggcat atttcctgat ttctgccata ctgaatctga aaacaggctc
    14941 agactgcatt tttttatccg tcaggtcaaa agaataaatc agcctgaaat cagaacaggg
    15001 agaatccttt ttcggaactt ccttgggtgc aacaagaagc ctgttgtttt tctgatcatg
    15061 gcagagccct tcattatctt tccagggaat atttgtttta taaatggttc tcttaaactt
    15121 atctttatcg tagtcagcaa tttcagtgag cattccatca ctgcgcagga ggtaaattgt
    15181 cctgccgacc ctggctatcc cttcataatc acctttatga ccgctaaaga tctgccttgt
    15241 tatctggttt ttgctgatgt cgtagatata aaccgcttca tgttcatctt caacacaggc
    15301 tattgttgag ccatcaagga cggcgattcc cgagatctcc agaaggaccg gaggcagaat
    15361 ataaaccttg tcaggagcag agagatcata tcccggatct gatcttcctt cagctgattt
    15421 attaccggtc tgtgcatctg tcatcagggg atatattata aggaatatca gaagaagctg
    15481 tttcatgttg aattattagt tactggccgg catacagatg caaaataaca attaatcggg
    15541 gaacaacaga aaaatattga ttatgaagta tgatcatcag gcatttcagg tatcggatat
    15601 tgacagttca attgattttt acgtgaataa acttggattc aggctttgtt caaaggaaac
    15661 ggataataat atactggagt ttattcagcg atataataaa aagtgatatc agccggcaag
    15721 caggatctgg ctgcctgtct ccatacagct tcaattcata aaggaagggg cgttacatta
    15781 ctgacttctc tctcagaact tctgtattct ttaacatgta ttctgtaaca tgtaatctcg
    15841 aatatatatt ctgtaatttg cctgattttt attt
//
LOCUS       DAOSPX010000031        15715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3850_length_15715_cov_4.538761, whole genome shotgun sequence.
ACCESSION   DAOSPX010000031 DAOSPX010000000
VERSION     DAOSPX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15715
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3850_length_15715_cov_4.538761"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PLZ75_02825"
     CDS             complement(<1..1246)
                     /locus_tag="PLZ75_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOS71364.1"
                     /translation="MKMNDIRDINQERPNRVNQPKNQFDLISLVPLVLKNWYWFVISI
                     AIALFLGRFYINHTLPVFRTYATVLINETEDRPLVDNSELLTGLGLPGGMRNLENQIA
                     ILKSRSLTKLTLEELPFEIEYYFQATRNKLPVYPEQPIRVVIDSGAPLPNDIDFSIKY
                     LGDDRFNIESGSEFFPLNMNTSFGEIIEMPEGKFSIECRNYDWLISNSHRDFRFVINS
                     PQQLISHYAYRLSVDRISREGSMLRLSLEGTNRAKDVDFINKQIEGFQAISLARKNAE
                     VERRLQFIDSQLVGISDSLSTTETRLQQFRSSHQVMDISAQGQSIIAQVTLLENERAR
                     LSLEANYYDYLADYLSKETSEEIPIVPITMGINDPGLTRLVEELAELQGQLSTRGAGE
                     MNPLQRNLEKRVRTAKDALKETL"
     gene            complement(1323..2114)
                     /locus_tag="PLZ75_02830"
     CDS             complement(1323..2114)
                     /locus_tag="PLZ75_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOS71365.1"
                     /translation="MKKYKSVYLILITIIFISGSCISKKKLMYLQYADQTSVYETPEG
                     AIASVIPSAYKIMPFDNLYIRVLTPDPQWSELFNAMPVGAGGAVTEESAALFGYPVDE
                     EGNIEIPFVGKITAGGKTLAEIKTELDLVFKNYLTDASITVRLVNNFVSVLGEVNAPG
                     RYLLTKDRVNVFEAISLAGDLSIFSNRRKVQLIRPSPYGPVVKEFSLADRNILSSELY
                     YIFPNDVIYVMPIQGRPFQTNSTVLTLFLSTISTALVIFSYIRTL"
     gene            complement(2389..3507)
                     /locus_tag="PLZ75_02835"
     CDS             complement(2389..3507)
                     /locus_tag="PLZ75_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOS71366.1"
                     /translation="MLKIGITGQSGFIGTHLYNFLGLKTDEIIRIPFEDSIFSNRRAL
                     EEFAGQCDVIVHLAALNRHNDPQEIYNTNIRLVKQLIEAFEATNSRPHVLFSSSIQEE
                     RDNIFGKSKREGRQLLSEWAERSGAAFTGMVIPNVFGPFGNPYYNSVVATFSHQLTHD
                     EKPKIDVDAQLRIIYIADLVKEIWNCIENRTNNKEYKIAHTNEIKVTEILQLLERFKS
                     DYFEKGILPDLNDPLERNLFNTFVCYIDHEKYFPFRLKLNTDERGSFVETVKLNSGGQ
                     ISFSTTKPGITRGNHFHTRKAERFAVIKGKARIQLRRVGTDRIIDFDLDGTRPSYVDM
                     PVWHTHNITNTGDEDLYTIFWISEFYNPDDPDTFFEQV"
     gene            complement(3645..4685)
                     /locus_tag="PLZ75_02840"
     CDS             complement(3645..4685)
                     /locus_tag="PLZ75_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOS71367.1"
                     /translation="MNFNGKTLLITGGTGSFGNAVLRRFLNTDISEIRIFSRDEKKQD
                     DMRHFYKNPKIKYFIGDVRDRMSIDNAMHGVDFVFHASALKQVPSCEFFPAEAVRTNV
                     LGCENVLDSAQHHHVKKVIVLSTDKAVYPVNAMGMTKALSEKIMVAKSRNLNGTGTVF
                     CGTRYGNVMASRGSVIPLFVNQMKHNQPITVTDPDMTRFMMSLESAVELVLFAFNNGN
                     NGDIFVMKSPATTIANLAQAIKELYKSESPIKVIGSRHGEKLYETLINREEMVKAVDM
                     GDFYRIPADTRDLNYNKFFTEGTQEVSMADEYNSHNTKRLTIEETKELLLTLRFIRQD
                     VLQEKQKIVYEP"
     gene            complement(4697..6673)
                     /locus_tag="PLZ75_02845"
     CDS             complement(4697..6673)
                     /locus_tag="PLZ75_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOS71368.1"
                     /translation="MSKTYPNWFLSLRRQVFNIFAKYYLPRWIVFTMDIIAVFLMFLC
                     AYLLRFNLDIDQMPFPLVWEQALLVIFVFGIMSFIFRSYAGLIRHTTLTDISLIFIVT
                     TLSTAVVLLLSYSARLFWPEFILLNIPVSVVIINFVLITVFLFSVRVIIKIIFRFVTS
                     SGRTKKRVLIYGAGELGFIVKRVILSDPENGFSVLGFIDDEKNLIGKKINQIPVFGYN
                     ALRRDFISKNRITSLILAIKDIDVEKKSRIIKLAVGRGLEVLETPEVSKWVNGQLQAR
                     QFQKVKLEDLLGRKPIKLNLDLIREGLDKKTILVTGAAGSIGSEIVRQLTRFNNEKLI
                     LVDDAETPLFYLENEIREKFTDLQARLILGDVTHPEKMEQVFRDFRPDIVFHAAAYKH
                     VSLIEENPHEALRVNVGGTRNMTELAVRYGVSKFVMISTDKSVNPTSVMGVTKRICEK
                     IVQASSLLELNRTQFIVTRFGNVLGSNGSVIPIFSNQIARGGPVTVTHPEVFRYFMTI
                     SEACQLVLEAGFMGKGGEIFVFDMGEPVKIADLARNMIRLSGLEPGRDIKIEYTGLRP
                     GEKLVEELLTDKEITKETHHPKIRIANVEPIDEFEILSKIDFLLQNLYSLTRKEAVKL
                     ISEIVPEYKSSNVRYNGYIASTEPVNGTQNSAHE"
     gene            complement(6985..7560)
                     /locus_tag="PLZ75_02850"
     CDS             complement(6985..7560)
                     /locus_tag="PLZ75_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71369.1"
                     /translation="MKKSLLGSLFFLSFTCIYAQDIISNPFDPKPGYININEVTAGFG
                     LGSTEVANSRFFYGFTTTHGYEFIIRPFGINSGLSMGAGGGMQFYDDAAYFPVFGDMR
                     YFVRLKTVSPFVSGSSGILVNPDNLRDQSMLFLNCGTGTMIKLSNNLNLSLGAGLFMQ
                     FSRTETRDTFLNFKAGVSFKPDRRLRNPNYN"
     gene            complement(8020..9189)
                     /gene="wecB"
                     /locus_tag="PLZ75_02855"
     CDS             complement(8020..9189)
                     /gene="wecB"
                     /locus_tag="PLZ75_02855"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOS71370.1"
                     /translation="MRVISVVGARPNFMKIAPFIRAVEKYNREHDNYIDHILVHTGQH
                     YDIRMSEAFFRALEIPEPCLNLEIGSGTHAEQLGHTMIEFEKVLRWEYPDWVVVVGDV
                     NATLSCSVVAKKFNVKLCHIEAGLRSGDMTMPEEINRLVTDRLSDLLFTPDLISSENL
                     RKEGVPEEKIFFAGNIMIDTLESNRIKAENLSVDKIISDNLIEGRKHSVDFSGNGDYA
                     LMTLHRPSNVDDEDVFFPLIKFLSEEVTKNFPLIWAVHPRARKQLEKNGWWDYVMSNP
                     ALIILEPVGYHEMLRLNMGARLMLTDSGGLQEECTVLGTPCITLRYNTERPVTLREHG
                     GLSVLTGNNLERIREEFLNALDIKRKPVRPELWDGHAAERIVEKLVEFDRRPEPR"
     gene            complement(9366..10670)
                     /locus_tag="PLZ75_02860"
     CDS             complement(9366..10670)
                     /locus_tag="PLZ75_02860"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOS71371.1"
                     /translation="MNITIVGTGYVGLVTGACFAEMGVNVTCVDKDKGKIEVLTDGGI
                     PIYEPGLEELVSKNMTAGRLKFSASLPEAMKDTEVIILAVGTPPDEDGSADLNNVLEV
                     AGEIGEHLERYVLVVNKSTVPVGTASKVEETIKKKLGERGAEIAFDVASNPEFLKEGS
                     AIKDFMTPDRVIVGTNSERAHEIMDRLYRPFMLNNYRVIYMDIPSAEMTKYAANAMLA
                     TRISFINEIANLCESVGADINMVRTGIGSDSRIGSKFLYPGVGYGGSCFPKDVKALIH
                     TAKLNGKQLRVLEAVEKVNELQKEVLFEKLIRHFGDDISGKKFAVWGLSFKPETDDVR
                     EAPSRVIIDRIISAGGTVSAYDPAAMDEFRKIRADINFSSDMYEACIDASAVLLVTEW
                     KEFRIPSWPTLKRIMASPVIFDGRNIYRRSDVTASGFVYYSI"
     gene            complement(10730..11695)
                     /locus_tag="PLZ75_02865"
     CDS             complement(10730..11695)
                     /locus_tag="PLZ75_02865"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOS71372.1"
                     /translation="MKVLITGAAGFIGSHACEHFIDSGYQVAGLDNFDDFYSRSIKEN
                     NLKTVKESDRFVFHEGDIRDESLLKKIFDNNSFDLVIHLAAKAGVRPSIYNVKEYYDV
                     NVNGTLNILEAMRVHNVKKLIFASSSSVYGNNSRLPFSESDPVDNPVSPYASTKKSGE
                     LLCHVYTHLYGFDVTCLRFFTVYGPRQRPDLAIHKFTRLIDEGKTVDLYGDGSTARDY
                     TYIDDIIEGIGRAAGRLKGYNIYNLGESDMIDLLSMVKVIEEYLGKKALLNYSPVQPG
                     DVKKTYADILKAKTELGYDPGYDFRKGMGKFIEWYRENRKPAAAR"
     gene            complement(11813..12958)
                     /gene="wecB"
                     /locus_tag="PLZ75_02870"
     CDS             complement(11813..12958)
                     /gene="wecB"
                     /locus_tag="PLZ75_02870"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOS71373.1"
                     /translation="MNKSSLKVLTVVGTRPEIIRLSRVMALLDQHFNHIIAHTGQNYD
                     YELNEVFFRDLELRKPDFFMNVDVSSLEASVGDIIRKSGELLRKEKPDALLVLGDTNS
                     CLSAYMAKRLHIPVFHMEAGNRCFDFNVPEELNRRIIDHIADFNLVYTEHARRHLISE
                     GLPHRRIYLTGSPMREVLQYYMNKIEHSSVLRELNLKTGEYFIVSVHREENVDHPANL
                     HRILSILNRLAGDYGFPVIVSTHPRTRKRLENLPGVKTDPLIQFLKPFGFIDYVHLQM
                     KALCTVSDSGTISEESSILNFPAISLRQSMERPEAQDAGTIILTGFDQQVVMDSIKLV
                     LKEFEQTGGYKKIADDYMIENTSWRVVKLISGNAGLSNIWNGIHHEN"
     gene            13662..15227
                     /locus_tag="PLZ75_02875"
     CDS             13662..15227
                     /locus_tag="PLZ75_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOS71374.1"
                     /translation="MKYILTLFISLAGFLVNAQTVQKIEGKTYTNNEDTWYGVNIPRK
                     DPTTLTFKNNSITSVNRYGYLLQAGDEVPGSYNNNLDGAVITGNRLKWNGTPEIGIIP
                     HGIFTGYNINVKVKYNYLDRIPMAIIRKSNGMTDVSGVVAYNIVKDPGVGVVVKGMNG
                     VKIYNNTFYSSLTPTETNRPLIHIYENTDISPVGSATGTKIYNNIFYCKHQIKNISIT
                     AACLPGFESDYNIFYCESGTPLFSVNGSLKTFAEWQALGYDTHSIVINPDFKDLTSFV
                     PKKRLDYGKNLGSEFATGLSPNVKWGTSDPATSAQNGKWQVGAIIHPEPENLPPVVSI
                     SLGDKGSDFTAPATIDINAMASDPDGFINKVEFYNGTAKLAESTTAPYSFTWKNVAVG
                     TYSLKAVATDNKNSKTTSDPVTITVSNMPTSVFNPSFSNMPSSKNNDRNQIRLYPNPS
                     NGIFKIDLSDPLLSDKNTVTIISLKGEILYTGLLMRDETDRQYDLSRAEKGNYIIMIK
                     SDQAVFTGKFIKN"
     gene            complement(15228..>15715)
                     /locus_tag="PLZ75_02880"
     CDS             complement(15228..>15715)
                     /locus_tag="PLZ75_02880"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS71375.1"
                     /translation="SGGLPSSFGSSSLADYKYSVYCSYRTGYSQTSGDANQNPKRAVK
                     DFAMMDDNTEKSSDTDDTGNDEMTLNLYPAEGTNKRMIVYPNPTSGNVTVSFEEASED
                     RHLLLIYDLQGRLVKTVNVEPWNYNIDVSIFDRKKGIYLFILKDMTNGTILNRSRVKK
                     N"
BASE COUNT         4514 a         3541 c         2963 g         4697 t
ORIGIN      
        1 tcaatgtttc ttttaaagca tccttcgctg tcctaaccct tttttccaga ttccgttgaa
       61 gcggattcat ttctcctgcc cctcttgttg acaattgccc ctgcagctca gcaagctctt
      121 ccaccagccg ggtcagccca ggatcattaa tccccatagt aatgggaaca atcgggattt
      181 cttcagaagt ttccttggaa agataatcag ccagataatc atagtaattt gcttcaagac
      241 taagacgtgc acgttcattc tcaagaaggg tgacctgagc aataatggat tgcccctggg
      301 cagagatatc cattacttga tgagatgagc ggaactgctg tagcctggtt tctgtggtgg
      361 aaagcgagtc agatatacct actaactgac tatcaataaa ctgcagcctt ctttccactt
      421 ccgcattttt tcgtgccaga gagattgcct gaaatccttc tatttgttta tttataaaat
      481 caacatcttt tgccctgttc gtgccttcaa gacttaatct cagcatagag ccctcccggg
      541 atattctgtc caccgaaaga cgataggcat aatgggaaat cagctgttgc ggactgttga
      601 ttacaaagcg gaaatcccgg tgactgttac ttatcagcca gtcatagttc ctgcattcaa
      661 tactgaactt accctcaggc atctcaatga tttctccaaa tgaagtattc atatttaaag
      721 ggaaaaattc ggaacctgac tctatattga aacgatcatc acccaggtac ttaatggaga
      781 aatcaatatc attcggaagc ggagctcctg aatcaattac aacccttata ggctgttccg
      841 gatatacagg gagcttgttc ctggtagcct gaaagtaata ttcaatctca aaaggcagtt
      901 cttccaaagt aagtttggtt aaagatcgtg attttaaaat agcaatctga ttttccagat
      961 tacgcattcc tcccggtaat ccgagccctg tcaataattc agagttgtct acaagcggcc
     1021 tgtcctcggt ctcatttatc agcacagttg catatgtccg aaaaacaggc aatgtatgat
     1081 taatatagaa acgcccaaga aacagagcaa tggcaatcga tataacaaac cagtaccagt
     1141 ttttcaaaac cagcggaaca agtgaaataa gatcaaactg attctttggc tgatttactc
     1201 tgtttggcct ttcctggttt atatccctga tgtcgttcat cttcatcccc catttaattt
     1261 catggtaatt attcctttgt gagacatttt cctttcagct aacttgcttt cggtcaatat
     1321 tattaaagag tccttatata actgaaaatc acaagagcag ttgatatggt agataaaaac
     1381 agtgtcagga cagttgaatt tgtctgaaaa ggtctgccct gaatcggcat tacataaatc
     1441 acatcattcg gaaaaatata ataaagttca gaagaaagaa tgttcctgtc tgcaagtgag
     1501 aattccttta caaccgggcc atagggagaa ggtcttatca actgcacctt cctgcggtta
     1561 ctaaatatac ttagatctcc tgccagcgaa attgcctcga aaacattcac cctgtccttg
     1621 gttaaaagat accttccggg agcatttact tcacccagaa cacttacaaa attatttacc
     1681 agtctgactg ttattgaggc atctgtcaga taattcttaa ataccagatc cagttctgtt
     1741 tttatttccg caagggtttt tcctcccgca gtaatctttc ctacaaaggg aatttcaata
     1801 ttcccttctt catccacagg atatccgaat aaagctgcgc tttcctcggt aacagctccc
     1861 ccggctccta ccggcattgc attaaacagt tcagaccatt gcggatcggg agtcagtacc
     1921 ctgatataaa gattgtcaaa tggcatgatt ttgtacgcag aaggtattac cgatgcaatc
     1981 gcaccttccg gagtttcata tacggaggtt tgatcagcat actgaagata catcagcttc
     2041 ttctttgata tacaggagcc tgatataaag atgatagtta ttaatatcag atagactgat
     2101 ttgtactttt tcattgtggt agtggcatta taatacaaaa atacacaaag aattgcttat
     2161 catgtttatt taattatctg aaacaacaat tgtcatcctg ttggctgatt ccgtattaca
     2221 ctccggtgat accggcaaat aattttcagc actgcatatt tattaacctc ccttattatc
     2281 cggccggaca atttatgcaa ttcttttttt actgtatatt cccatatacg taacatcagg
     2341 aaaaaagaca gcaaagcaca gaaatatttt tcaggttcaa attaagtttc agacctgttc
     2401 aaaaaaagta tccggatcat ccggattgta aaattcactt atccagaata tcgtatacag
     2461 gtcttcatcg cctgtattgg tgatattgtg ggtatgccag actggcatgt caacatagga
     2521 tggacgggtt ccgtcaagat caaaatcaat gatcctgtct gttcccacac gcctcagctg
     2581 aattctggct tttcccttaa tcacagcaaa acgctcggct ttacgggtat ggaaatgatt
     2641 cccgcgggta atacccggtt ttgtggtaga aaacgaaatc tggccgccgc tgttgagttt
     2701 taccgtttcc acaaatgaac ccctttcatc agtattgagc tttaaccgga agggaaaata
     2761 tttttcatga tcaatataac agacaaaggt attgaacagg tttctttcca aaggatcatt
     2821 aagatcaggt aaaattcctt tctcaaaata atcagatttg aatctttcaa gcagctgaag
     2881 tatttctgta acctttattt catttgtgtg ggcaatctta tattccttat tgtttgttct
     2941 gttctcaata caattccaga tctccttaac caggtcggct atgtaaataa ttctcagctg
     3001 tgcatctaca tcaattttcg gtttttcatc atgggtgagc tgatgcgaga atgtggcaac
     3061 aaccgagtta tagtaaggat tcccgaaggg accaaacaca ttgggtatca ccataccagt
     3121 gaaggctgct ccgcttcttt cagcccattc cgacagtaac tgcctaccct cccgttttga
     3181 ctttccgaaa atattatcac gttcttcctg aatggaagat gaaaaaagca catgtggcct
     3241 ggaatttgtc gcctcaaatg cttcgataag ctgttttacc agtctgatat tggtattgta
     3301 aatctcctga ggatcattat gcctgttgag agcagccagg tgaacaatta catcacattg
     3361 ccctgcgaat tcctccaatg cccgcctgtt ggaaaaaatg gagtcttcaa agggaatccg
     3421 gataatttca tccgttttta aacccagaaa attatataaa tgtgtgccga taaagcctga
     3481 ctggccggtt attcctattt taagcatatt atttttttca gggttagtaa taaaactctt
     3541 cgaaaccggt tccggaattg aggtcatggc tgttactctg ttcaggctta gtaataaaac
     3601 tcctcgaaac cggttccgga actgagattc tggctggttt ctgatcaggg ttcgtaaaca
     3661 attttctgtt tctcctgcag gacatcctgc cggatgaatc tgagtgtcag aagaagttct
     3721 ttcgtttctt caattgtcag gcgtttagta ttatgggaat tgtattcatc tgccattgaa
     3781 acctcctgtg tgccttcagt gaagaactta ttgtaattaa gatcgcgggt atcagcgggg
     3841 atcctgtaga aatcgcccat atccacggct tttaccattt cttcgcggtt gatgagtgtt
     3901 tcatacagtt tttcgccatg gcgggatcct attactttta ttggtgattc tgatttataa
     3961 agttctttta ttgcctgtgc cagattggcg atggttgttg ccggcgattt catcacaaag
     4021 atatctccgt tgttgccatt gttgaaagca aacaaaacca gttcaaccgc actttcaagg
     4081 ctcatcatga acctggtcat atccggatca gtaacggtaa taggctggtt atgcttcatc
     4141 tgattgacaa ataacggtat taccgatcct ctggaagcca tgacgttgcc atatcttgta
     4201 ccgcagaaaa cagttcccgt accgttaaga ttcctcgatt ttgcaaccat tatcttttcg
     4261 ctgagcgctt tggtcattcc catggcatta accgggtaaa cagccttgtc ggtactcaga
     4321 acaattacct tttttacgtg atgatgctgt gccgaatcga gaacattttc acagcccaga
     4381 acgttggtcc tgacagcctc agccgggaaa aactcacagc tcggaacctg cttcagtgct
     4441 gatgcatgaa aaacaaaatc aacaccgtgc atggcattgt caatactcat cctgtcgcgg
     4501 acatctccta tgaaatattt gattttggga tttttataaa aatgccgcat atcatcctgt
     4561 ttcttttcat cgcggctgaa aatccgtatc tccgaaatat ctgtattcaa aaaacgccgc
     4621 aaaacggcat ttccgaatga ccctgtacct cctgtgatga ggagggtttt tccgttaaaa
     4681 ttcatattat aaaattttat tcgtgagctg aattctgcgt tccgttaacg ggttccgtgc
     4741 tagctatgta tccgttgtaa cgcacattgc tggatttata ttccggtact atctccgaaa
     4801 tcagcttaac agcttctttt ctggttaaac tgtataagtt ctgtaaaagg aaatctattt
     4861 tggaaaggat ttcaaactca tctatgggtt caacattcgc aatccggatt ttgggatgat
     4921 gggtttcctt cgttatttcc ttgtcagtaa gcaattcctc gacaagtttt tctcccggac
     4981 gtaatccggt atattcgatc ttaatatccc ttcccggttc cagtcctgac aggcggatca
     5041 tatttcttgc cagatctgct atctttacag gttctcccat atcgaaaaca aatatctccc
     5101 ctcccttccc catgaatcct gcttcaagaa caagctgaca ggcctctgat attgtcatga
     5161 aatacctgaa tacttcagga tgggtaactg ttaccggacc gcctctggct atctgattgg
     5221 agaatatcgg tataaccgaa ccatttgacc ccaggacatt tccaaaccgt gtaacaatga
     5281 actgggtcct gttcagttcc agcagggaag acgcctgaac aatcttctcg catatccgtt
     5341 ttgtaactcc catcacgctg gtgggattca ccgatttatc agtagagatc atgacaaact
     5401 tgctgactcc gtacctgact gccagttccg tcatgttcct ggtacctcct acattcaccc
     5461 tcagtgcttc atggggattt tcttcaatca gggatacatg cttgtaagct gcagcatgaa
     5521 aaacaatatc cggacggaaa tccctgaaga cctgctccat cttctccggg tgtgtcacgt
     5581 caccaagaat cagcctggcc tgaagatcag taaatttttc ccttatctca ttttcgaggt
     5641 aaaagagagg tgtttccgca tcatccacca gaatcagttt ttcattattg aaacgggtaa
     5701 gctgacgtac aatttctgaa ccgattgatc cggcggcacc tgttaccaga atggttttct
     5761 tgtcgagtcc ttctcgtata agatccagat tcagtttaat aggctttctg ccaagcagat
     5821 cttccagttt tactttctgg aactgtctcg cctgcaactg accattaacc catttactta
     5881 cttcaggtgt ttccagaact tcaagtccgc ggcctacggc aagtttaatt atcctgcttt
     5941 ttttctcaac atcaatatcc ttaatagcta aaatcagact ggtaatcctg ttctttgaaa
     6001 tgaaatccct tctgagcgcg ttatagccaa aaaccggtat ctgattgatt ttttttccta
     6061 taagattctt ttcatcgtca ataaaaccca agacactgaa tccgttttca ggatcactga
     6121 gaatgacacg tttcacaatg aatcccagtt ctccggcacc atatatcagt actcttttct
     6181 ttgtccttcc cgaggaagtc acgaaacgga aaataatttt aattatcacc cgcaccgaaa
     6241 aaaggaatac cgttatcagt acgaagttta ttatgaccac tgaaacagga atattcagca
     6301 ggataaattc cggccagaac aacctggctg aatatgataa gagcagaaca acagcggtag
     6361 aaagagttgt aacaatgaat atgagtgaaa tatccgtcag ggtcgtatga cgaatgagtc
     6421 ccgcgtatga acggaatata aaactcatta ttccaaagac aaaaataaca agcaatgcct
     6481 gttcccatac caggggaaac ggcatctgat ctatatccag attgaatctc agcagataag
     6541 cgcaaagaaa catcaggaaa accgcaatga tatccatagt gaatactatc caccgtggca
     6601 gataatactt cgcaaatatg ttaaacacct gccggcgaag agaaaggaac cagtttgggt
     6661 aagttttaga catcagctaa attatctgtt ttaaatagtc cgggtataat ttttaggatt
     6721 cagtttccat atccggttca tcgctaaaaa tcagccaagc taaccaaaaa tttaacatgc
     6781 tgactatatt ttaaacgaac cacttaaatt actttaccaa catgcaaaat aatttgataa
     6841 ccggcaaaat ttctattatc cggcaaaacg ctttttttac ctgcaaagct atttttaaac
     6901 ctgataactt taaatttatc tgtataaccc atcggcttgc cgctttggtc tattccatat
     6961 ccccgattca gtaatacaaa gatattaatt gtaattcgga tttctgagtc ttcggtcggg
     7021 tttaaagctt actccggctt tgaaatttaa aaaagtatct cttgtttcag tccggctgaa
     7081 ttgcataaaa agaccggcac ccagcgacag attgagatta ttactcagtt ttatcattgt
     7141 acctgtacca cagttcagga agagcatcga ctgatcgcgc agattatcag ggttaaccag
     7201 tatgccgcta ctcccggaaa caaagggaga aacagtcttt aatctgacaa aatatctcat
     7261 atcgccaaaa accgggaaat atgcagcgtc atcataaaac tgcattcctc ccccggcccc
     7321 catggaaaga ccgctgttta taccgaaagg cctgataata aactcataac catgagttgt
     7381 cgtgaagcca taaaaaaacc gggaattggc cacctctgta cttcccagcc caaaacctgc
     7441 tgtcacctca tttatattga tatatcccgg tttcggatca aagggattgg aaattatgtc
     7501 ctgcgcgtat atgcaggtaa aactcaaaaa gaacagactg cccaaaagag attttttcat
     7561 tcctgaaaca ttcgttgcaa acggcttgac ttacttaatg aaatattgca aataactaaa
     7621 atctgcttgt ttaattatca aaattttact attccttaga tcagggcatg cttccgcccc
     7681 gtgagcccca ctcttaaaag gtccatttat ctgatccagt gtctgttaaa agcaatattt
     7741 gatttaagta aatcaatttc aggaaatatc acacagatcc gcatggtccc gggatcccct
     7801 ttgcagatac gaaattaaac aatgctactc tatcttcaaa atgcttaata ttaacagaca
     7861 atagcaaata tagccgttct gaagccaact ggacttttgt tttatcaaca aatcaaaagt
     7921 gattgttaat atgcttaata atcaaaaaag gcaatcagta tatctgtaat ccaaaaaaca
     7981 gaaaaaatat tgaaaatccg gaagttagga tatgcaggtt tatcgaggtt ccggtcttct
     8041 gtcaaactct acaagctttt ccactatccg ttctgccgca tgtccgtccc agagttccgg
     8101 tctcacaggc ttccgcttaa tatcgagagc attcaggaat tcctccctga tcctctccag
     8161 gttatttccc gtaagcacac tcagtccgcc gtgttcccgc agggtaacag ggcgttcggt
     8221 attataacgc agtgtgatgc atggggttcc cagaacggta cactcttcct gaagtccgcc
     8281 ggaatcagta agcatcagcc tggctcccat attcaggcgc aacatttcat gatagcccac
     8341 cggctcaagg attatcagag caggattgct catcacgtaa tcccaccagc cgtttttttc
     8401 aagctgtttc cttgcacgcg gatggactgc ccagatcagc ggaaaattct ttgtcacctc
     8461 ttctgacagg aatttgatca gcggaaaaaa tacatcctca tcatccacat tggacggacg
     8521 gtgaagggtc atcagagcat agtcgccatt tccggaaaaa tcgacagaat gtttacggcc
     8581 ctcaataaga ttatccgaaa tgattttatc aacagacaga ttttccgcct ttatcctgtt
     8641 tgattcaagg gtatctatca ttatgttgcc ggcaaagaat attttttctt ccggcacccc
     8701 ttcctttctc aggttttccg aagaaataag atcaggagta aaaagaagat ccgaaagcct
     8761 gtcggttacg agccggttta tttcttccgg catggtcatg tcccctgaac gaaggcctgc
     8821 ctcaatatgg cataatttta cattgaattt ttttgcaacg actgaacatg aaagtgttgc
     8881 attaacatca cccacaacaa ccacccagtc gggatattcc cagcggagca ccttttcgaa
     8941 ctctatcatg gtgtggccca gctgttcggc atgggttccc gatccgatct caaggttaag
     9001 acagggttca ggtatctcaa gagccctgaa aaatgcttcc gacatcctga tatcataatg
     9061 ttgtccggta tgtaccagga tatgatcgat ataattatcg tgttcccggt tatatttctc
     9121 aacagctctg ataaaaggag ctattttcat gaaattcgga cgggcaccta caactgatat
     9181 tactctcatc agtaaattat tttggtttta ttgttacgac agtctgcacc aactctgcag
     9241 taaaaagggg atcttccaga agttccggga tatccggtgt aagctgatga atgttaagac
     9301 ttaaaaaacc tgagtgccag aaaaatccgg aggtatcata tcccggtatt taagatccgg
     9361 cattatcaga tactgtaata aacaaatccg ctggctgtaa cgtctgaacg cctgtatata
     9421 tttcttccgt cgaaaattac cggtgaagcc ataattcttt tcagagtggg ccacgaagga
     9481 atacggaatt ccttccattc cgtcacaagc agaacagccg aagcatcgat acaggcttca
     9541 tacatatcac tgctgaaatt tatatcagcc cttatcttcc tgaattcatc cattgctgca
     9601 ggatcatacg ccgaaacagt tccgcctgcg ctgattatcc tgtcaattat caccctggag
     9661 ggagcttcgc ggacatcatc cgtttcaggt ttgaatgaca gcccccatac agcaaatttt
     9721 tttcctgaaa tatcgtctcc gaaatgcctg ataagctttt cgaacaagac ttccttctgc
     9781 aattcattta ctttctcaac tgcttccaga accctgagct gttttccatt cagcttcgcc
     9841 gtatgaatca gggccttaac atctttcggg aaacatgatc ccccgtaacc tactcccggg
     9901 taaaggaatt tgcttcctat cctgctgtcg gaacctattc ccgttcttac catatttata
     9961 tctgccccga cactttcaca cagattggca atttcgttaa taaagcttat ccgggttgca
    10021 agcattgcat ttgcagcgta tttcgtcatt tcagccgaag ggatatccat atatatcacc
    10081 ctgtaattat tgagcatgaa cggcctgtac aaacgatcca ttatctcatg ggccctttcc
    10141 gaattagttc cgacaatcac acggtccggt gtcataaaat cctttatggc cgaaccttcc
    10201 ttaaggaatt ccggatttga ggcaacatcg aatgcgattt cagctcccct ttcccccagc
    10261 ttctttttta tggtttcttc aacctttgat gcagtgccta ccggaaccgt acttttgttc
    10321 acaaccagaa catatctctc caggtgttct cctatttctc ctgctacttc aagaacgttg
    10381 ttcagatccg cactgccgtc ttcgtccggc ggagtgccca ctgccaggat aatcacctct
    10441 gtatctttca ttgcttccgg cagggaagca gagaatttca gtctgccggc agtcatattc
    10501 ttgcttacaa gttcttcaag tcctggctca taaatcggaa taccgccatc agtaagaact
    10561 tctattttcc ctttatcctt gtcaacacag gtgacattta cacccatctc cgcaaaacat
    10621 gccccggtta ccagaccgac ataacctgtg ccaacaattg taatattcat aaacctattt
    10681 tttaaacaat aaccgtttta ctctgtgagg tccttccggg aaggaaacct taccgggcag
    10741 cagccggctt tctgttttcc ctgtaccatt caatgaattt ccccattcct ttcctgaaat
    10801 catatcccgg atcatacccg agttcagtct tagctttcag aatatcggca taagttttct
    10861 ttacatcacc gggttgcacg ggtgagtaat tcaatagtgc cttttttcca aggtattctt
    10921 caataacctt gaccattgaa agcaggtcaa tcatatccga ttctcccaga ttataaatat
    10981 tgtaaccctt cagccggcct gcagccctgc cgattccttc aatgatatca tcaatatagg
    11041 tataatctct tgctgttgat ccgtctccgt ataaatcaac cgttttccct tcatctatta
    11101 gtctcgtaaa cttatgaatg gccagatcgg gcctctgacg gggaccgtaa acagtgaaaa
    11161 atctcagaca tgttacatca aaaccgtaaa gatgagtata aacatgacac agcagttctc
    11221 cgcttttttt tgttgatgca tagggcgaaa ccggattatc cactggatct gattctgaaa
    11281 acggcagtct ggaattattg ccatatactg atgaagatga ggcaaatata agttttttta
    11341 cattatggac cctcattgct tcaaggatat tcagggttcc gtttacattg acatcataat
    11401 attctttcac attataaatg gatggcctta ctcccgcttt tgccgcaaga tgaataacca
    11461 gatcaaaact gttgttgtca aatatttttt ttaacaggga ttcatctctt atatcccctt
    11521 catgaaacac aaagcggtca gattctttaa cggtcttaag attattctcc ttgatgctcc
    11581 tggaatagaa atcatcaaaa ttatccagac cagcaacctg atacccacta tcaataaaat
    11641 gttcacaggc atgtgagcca atgaatcctg cagcaccggt aatcaatacc ttcattctat
    11701 accttgttta tttttgtgtt tctggtataa taacatgacc atgcgccgga taccctgaat
    11761 tctcataaaa acattagttt atgcctgaat atctgatggt gaaaacgagc tgttaatttt
    11821 catgatgaat cccgttccat atattgctta gtccggcatt tcctgaaatc agtttcacga
    11881 cacgccatga tgtgttttca atcatataat catctgcaat ttttttgtat cccccggtct
    11941 gttcaaattc cttcaggacg agcttaatgc tgtccatcac gacctgctga tcaaaacctg
    12001 tcagaatgat tgttccggcg tcttgtgctt caggtctttc cattgactgg cgcaatgaaa
    12061 tggccggaaa attgagaatg gacgattctt cacttattgt tcccgaatca gaaaccgtac
    12121 aaagggcttt catctgaaga tgaacataat cgatgaaacc aaagggtttg aggaactgta
    12181 tcagcggatc cgtcttcaca ccaggaaggt tttcaagcct ttttcttgta cggggatgtg
    12241 ttgatactat aacaggaaat ccgtagtcgc cggcaagccg gttcaaaatg gacagaatcc
    12301 tgtgaagatt ggcaggatga tcaacatttt cctccctgtg gactgatact atgaaatatt
    12361 cgcctgtttt cagattcagt tccctgagga cggaagaatg ttcaatctta ttcatgtaat
    12421 attgcagcac ttctctcatc ggactgcccg taagatatat cctcctgtgc ggcaatccct
    12481 ccgatatgag atgccgccgt gcatgttcag tgtaaacaag attgaagtca gctatatggt
    12541 ctattattct tctgttaagt tcctccggca cattaaagtc gaaacaacgg tttccggctt
    12601 ccatatgaaa aacaggaata tgaagccttt ttgccatata ggccgacagg caggaattgg
    12661 tatctccaag taccagcaaa gcatcaggct tttcctttct cagcagttct cccgatttcc
    12721 ggattatgtc tcccactgag gcttcgaggg atgaaacatc aacattcata aaaaaatcgg
    12781 gtttacgaag ttccaggtcc ctgaaaaaaa cttcattcag ttcataatca tagttctgtc
    12841 cggtatgggc tataatatga ttgaaatgct gatcgaggag tgccatcact cttgagagac
    12901 ggattatttc cggacgagtt ccgacaactg tcagaacttt aagggaggat ttattcatgt
    12961 tctgattctg tgacaactgg tgagttttta tggtttataa tgaatagtaa aaaagattgt
    13021 tcgggtacaa atgtaacaag ttgtgaaatt ctgttactga tattctgaat tttctgcatt
    13081 tattgtctgt ttctcttggg tccgggcatg cttccgcccc gtcagcccca tgagttttct
    13141 gatataacca attgaatata tgatctaaag atcccggcca gagaagtccg gaattttaaa
    13201 tcatattcaa tttatccctc attttctgac tatgtaaatg caataataca atatatgaaa
    13261 aatcaaatgt ttaaacgatg ggagggtgaa gatgttcgta aaagttgaaa aaattacgga
    13321 aacaggcagt ttttattgat gagggtggga ttatacccgg taagaaccgg gaaatgttta
    13381 tctgttttga cagcattttc ccctgccgca ccatgaaaac aggaatggaa aaagaagttt
    13441 cgcttcatat tcttttatcc atttcatgtc ttgctgcagt tgatcaagca ggactagctt
    13501 aatcatcatg tttctgtttc agaaggttaa ggaatattga caagtatcaa tccacagcta
    13561 tgaaattttt atgcttttgt gccgttaatg taacaccggc agttgtccga cgtataaaca
    13621 ggggtgattg tcaaaagtta tatacatttt aattgaattg catgaaatat atactaacac
    13681 tatttatttc attagccgga ttcttggtta atgcccagac agtacagaaa atagaaggaa
    13741 aaacatatac gaacaatgag gatacatggt atggagtgaa tattccacga aaagatccca
    13801 caacattaac ctttaaaaac aattcaatta cttcagtgaa ccgttacgga tatcttctcc
    13861 aggccggaga tgaggttccc ggatcataca acaacaatct tgacggtgca gttatcaccg
    13921 gaaaccggtt aaaatggaac ggaaccccgg aaattggaat tattcctcat ggcatattca
    13981 ccggatataa tattaacgta aaggtaaaat acaactatct cgacaggatt ccaatggcaa
    14041 taatcaggaa gtcaaacggg atgacagatg tatccggtgt ggtagcatac aatattgtaa
    14101 aggatcccgg tgtcggagtt gtagtgaagg gcatgaacgg tgtaaagata tacaacaata
    14161 ccttttacag ttcattaacc ccaacggaaa caaaccgccc gcttatccat atttacgaga
    14221 acacagacat cagccctgtg ggttcagcaa caggtaccaa aatatataac aatatttttt
    14281 attgtaagca tcagataaag aatataagca taacagcagc ctgcctgccc ggatttgaaa
    14341 gtgattataa cattttttac tgtgaatccg gtactccgct cttttcagta aacggatccc
    14401 tgaagacatt tgccgaatgg caggccctgg gatatgacac tcattctatt gtaataaatc
    14461 ctgattttaa ggatcttacc agttttgttc cgaaaaaaag acttgattat ggcaaaaacc
    14521 tgggttctga atttgccaca ggactttccc cgaacgtaaa atggggaaca agtgatccgg
    14581 caacttctgc tcagaacgga aaatggcagg ttggtgcaat tatccatcca gaaccggaaa
    14641 acctgccccc ggtggtcagt atctctcttg gagacaaagg atcagacttt acagctcccg
    14701 ctacaattga tattaatgcc atggcatccg atcccgatgg ttttattaac aaagtggaat
    14761 tctataacgg gactgccaaa ctcgccgaaa gcactacagc tccttactct ttcacctgga
    14821 aaaatgtagc cgtaggaaca tattcgctaa aagctgttgc taccgataat aagaacagca
    14881 aaaccacctc ggaccctgtt accataacag tcagcaacat gccaacttct gtctttaatc
    14941 cgtcattttc aaatatgccg tcatcaaaaa acaatgacag aaatcagatc aggctttatc
    15001 ccaatccttc aaacgggatt tttaagatag atctgtccga tcctctttta tccgataaaa
    15061 atacagtaac aataataagt ctgaaaggag agatcctcta tacaggattg ctgatgaggg
    15121 atgaaacaga ccggcagtac gatttatccc gtgcagaaaa gggaaattac attatcatga
    15181 tcaaaagtga ccaggctgtc tttaccggaa aatttattaa aaactaatca gttctttttt
    15241 actcttgacc tgttaagtat cgttccgtta gtcatatcct tcaggatgaa cagatatatt
    15301 cctttcttcc tgtcaaaaat actgacgtca atattataat tccagggctc aacatttact
    15361 gtttttacaa gtctgccctg caaatcataa atcaataata agtgcctgtc ctcagatgcc
    15421 tcttcaaaac tcacggtaac attaccgctg gtaggatttg gatatacaat cattcgctta
    15481 ttcgtacctt ccgccggata cagattcaaa gtcatttcat catttccagt atcatccgta
    15541 tcagatgatt tttctgtatt gtcgtccatc attgcaaaat ctttcactgc tcttttggga
    15601 ttctggtttg catcaccgga agtctgggaa tatcctgttc tgtagctgca atacacagag
    15661 tatttgtaat cagccaggct ggatgaacca aagctggagg gcagaccacc agacc
//
LOCUS       DAOSPX010000032        15700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3857_length_15700_cov_4.161202, whole genome shotgun sequence.
ACCESSION   DAOSPX010000032 DAOSPX010000000
VERSION     DAOSPX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15700
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3857_length_15700_cov_4.161202"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1333)
                     /gene="rocD"
                     /locus_tag="PLZ75_02885"
     CDS             complement(122..1333)
                     /gene="rocD"
                     /locus_tag="PLZ75_02885"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOS71376.1"
                     /translation="MKTQDYIEREEKYGAHNYHPLPVVLERGEGPYVWDVEGKRYFDF
                     LSAYSAVNQGHCHPEIIKALKDQADRLTLTSRAFYSSVLGEYEEYITRYFGYDKVLPM
                     NSGAEADETAIKLCRKWAYKKKGIEQNKAKIIACEGNFHGRTITIISMSTDPDSTGDY
                     GPFTPGFLIIPYNDTDALEEALQDPDVAGFLVEPIQGEAGVVVPDEGYLRKSYELCKK
                     HNVLFIADEIQTGLCRTGKLLACDHEGVRPDILVLGKALSGGVMPISAVLADDDIMLT
                     IKPGEHGSTFGGNPLAGKVGIAALEVLKNEKLSENAVRLGEIFRKEMKAVKSDMIETV
                     RGKGLLNAVVIRPKGGKTAWDVCIAMKDKGVIAKPTHGHIIRFAPPLVINEEQMAEAI
                     SLIKDAFKLFE"
     gene            complement(1615..2295)
                     /gene="trmD"
                     /locus_tag="PLZ75_02890"
     CDS             complement(1615..2295)
                     /gene="trmD"
                     /locus_tag="PLZ75_02890"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOS71377.1"
                     /translation="MRIDILTVSPELLESPLNFSIIKRARDKGVADINVVNLHDFAKD
                     RYRSVDDYAFGGGAGMVLMIEPVWKAIEKLRSERDYDEIIYASPDGMMFDQKMANRLS
                     LKNSIMILAGHFKGVDQRIRDHLVTMEISIGNYVLSGGELPAAVITDAIVRLLPGAIS
                     DEQSALSDSFQDDLLAPPVYTRPADFRGWKVPDILLSGNAREIDKWRHEQSVSKTKRL
                     RPDLAGDE"
     gene            2358..2600
                     /locus_tag="PLZ75_02895"
     CDS             2358..2600
                     /locus_tag="PLZ75_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOS71378.1"
                     /translation="MECRPGCGACCTVISISSPIPGMSEGKPAGVPCIHLLEDYRCAL
                     YGDPSRPPVCLNYRAEPEFCGNSREEAIRILTSLEV"
     gene            complement(2632..4320)
                     /locus_tag="PLZ75_02900"
     CDS             complement(2632..4320)
                     /locus_tag="PLZ75_02900"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOS71379.1"
                     /translation="MNRILLLLSVTVMVLTFSCGQPEAADLVILNGKILTIDSSNPLC
                     EAVAVKEGKISATGKTNKIRKFINPGETRVIDASGRLVIPGFNDAHLHFGPLDPDYIE
                     LRYTTDPAVITEKVKARVAGSKPGEIIRGGHWDHEMFTTREWPSKELIDKVSPDNPVI
                     LSRADGHSVLVNSYVLRKSGITKNTPDPFGGEIQKDPVTGEPTGILKETAMGLAKTGE
                     VKQELTDEEIAQKTWQGYLLAMKHARELGVTSVQNAGSADFEAYEKLQEAGELTCRID
                     IGRTLTADPDILRSYRELEEKYPDEGNWIRFGFLKAFIDGTMGSGTALMHEPYDDEPG
                     TSGLAMWKYDEFEEMVLAADKMGFQIAVHAIGDKGNTWVLDAFEKATEVNGMRDSRHR
                     DEHAQTLTPSDIPRFARLGVIPSMQPTHCITDKRFCEKRIGTERSAGAYAWRSLLDAG
                     AVLAFGTDYQVEPLNPMEGLYAAVTRKDRKGEEGDGWFPEQKLSMEEAIKYYTWGSAY
                     AQFMEDRKGMLKPGYLADMVIVDRDLLTIPENEIMQTKVDYTIVDGKVVYESGK"
     gene            4457..5176
                     /gene="dapB"
                     /locus_tag="PLZ75_02905"
     CDS             4457..5176
                     /gene="dapB"
                     /locus_tag="PLZ75_02905"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOS71380.1"
                     /translation="MNIVLIGYGRMGHEIEEAAVKRGHTIVLAIDADNLEDLNEENMK
                     EADVAIEFTMPEAAFGNISACLKMGVPVVSGTTGWLKDYDRARELCIENGTSFLHSSN
                     FSIGVSLLYRLSSELAEYMKHYSEYSVSIEEIHHVKKLDAPSGTAIALAEGIAGRHPG
                     YAAGWCPDQERKEGMIPVKSVREGLVPGTHIITWDSDIDTISLKHEAKNRKGLALGAV
                     VAAEFISTRKGVFTMKDVLGF"
     gene            5217..6605
                     /locus_tag="PLZ75_02910"
     CDS             5217..6605
                     /locus_tag="PLZ75_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOS71381.1"
                     /translation="MNKFLQKKYIMFVIAALIFLFVVVWIGNFWLLLGLPVIFDIYVS
                     KKVNWTFWKRRDRNNSAFIEWLDAIIFAVIAVTLINIFLFQNYRIPTPSMEKSLLVGD
                     HLFVSKLAYGPRMPNTPLAFPFTQHTLPLTKSKSWTDVVYWPYKRLAGLGHVKRNDPI
                     VFNFPAGDTVVVEEQATSYYEIVRRTARELQARDSYGNPEPKALEYYLPAARKEIRSR
                     YEVIYRPADRRDNYVKRCMALPGDTLEIRAGEVYIDGRPVEENKTQQTTYIVATNGTT
                     INPKAFERLGISRSDVNMLSGTAYILPLTKSSAEAISKFPNVLSTTAVYARKGEYSPQ
                     IFPYDPVRAWNEDNFGPLWIPRKGVEVKIDTSNICLYERIIEVYEENDLRTENGRIYI
                     NGVESDSYTFKMDYYFMMGDNRHNSADSRWWGFVPEDHIVGKPKIIWLSLNKEGKGFR
                     KIRLNRMFMKIK"
     gene            complement(6659..7006)
                     /gene="rplT"
                     /locus_tag="PLZ75_02915"
     CDS             complement(6659..7006)
                     /gene="rplT"
                     /locus_tag="PLZ75_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOS71382.1"
                     /translation="MPRSVNAVASRARRKKILRQTKGNYLGRKNLITVAKDTLYKGLG
                     YAYRDRRRKKGEFRSLWIRRINAGVRQYDMSYSEFIGKLDKKGIALNRKTLADLAMNH
                     PEAFRAIVEKVKE"
     gene            complement(7124..7321)
                     /gene="rpmI"
                     /locus_tag="PLZ75_02920"
     CDS             complement(7124..7321)
                     /gene="rpmI"
                     /locus_tag="PLZ75_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOS71383.1"
                     /translation="MPKMKTNPGAKKRFSFTGTGKIKRKHAYKSHILTKKSTKRKRNL
                     TYFGQVDKADMNNIKLLMVKK"
     gene            complement(7340..7894)
                     /gene="infC"
                     /locus_tag="PLZ75_02925"
     CDS             complement(7340..7894)
                     /gene="infC"
                     /locus_tag="PLZ75_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOS71384.1"
                     /translation="MAIPVKRYPGKVLQPAHRINNKITAKVVRVVGDEIDAEIMSVQD
                     ALKLADRMELDLVEISPNADPPVCKIIDYQKFLYQQKKKQKEIKAKAARVIVKEIRFG
                     PNTDDHDYNFKLRHAIKFLEEGAKVRAYVFFKGRSILFKEQGELLLLRFASDLEEYGK
                     VDQLPKLEGKRMIISFSPKKKKKT"
     gene            complement(7940..8170)
                     /locus_tag="PLZ75_02930"
     CDS             complement(7940..8170)
                     /locus_tag="PLZ75_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71385.1"
                     /translation="MDLGKRRESRRVQEKSEEKRRIKDWRTEESWEEQRRVVKNRGET
                     GRKKENRKELMNIENNGIKPGSTGKRGGKQGA"
     gene            complement(8219..10159)
                     /gene="thrS"
                     /locus_tag="PLZ75_02935"
     CDS             complement(8219..10159)
                     /gene="thrS"
                     /locus_tag="PLZ75_02935"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOS71386.1"
                     /translation="MIKITFPDGTSKEYREGITSLEIAGEISPRLAKAVYSATVNGEV
                     RDLTMPLRSDSTLRLNKWEDDEGRHAFWHSSAHLMAEALESLYPGIKFGIGPAIENGF
                     YYDVDPGDGVSITDSDLPAIENKMKELAAMNLTYKRKEVSKAEAMELFTRKGDEYKTE
                     LIGELEDGTITLYSNGNFTDLCRGPHLPSTEPIKAIKLTSVAGAYWRGDEKRKMLTRI
                     YGITFPEQKQLKEYLDFLEEAKKRDHRRLGKELELFTFSPKVGLGLPLWLPRGSDVRQ
                     NLINYLTSLLRKRGYKIVTTPHIGNVNLYKLSGHFEKYRTSSFQPISTPEEGEQFMLK
                     PMNCPHHCEIYNATPHSYRELPLRMAEFGTVYRYEQSGELHGLTRVRSFTQDDAHHFC
                     RPDQLREEFKSIIDLVLYVFKILNFNDFTAQISLRDPEDKDKYIGSDENWERAEQDII
                     EAAREKNLKTVVVPGEAAFYGPKLDFMVKDAIGRKWQLGTIQVDYNLPERFDLTYNGS
                     DNQKHRPVLIHRAIFGSLERFVAVLIENTAGKLPLWLSPEKAVILPISEKFNDYASNV
                     LEILNNSDICTLIDDRSEKIGKKIRDNELKRIPYLLVIGEKEAESGTVSVRKQGEGDK
                     GSMKIGDFIAFIRSEISKELAD"
     gene            10369..11118
                     /locus_tag="PLZ75_02940"
     CDS             10369..11118
                     /locus_tag="PLZ75_02940"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOS71387.1"
                     /translation="MKKIIFLLSFLPVFLFSCEKTPEAYFTVDKTGPEVGQEVHFKNK
                     SRNAVEYEWDFGDGFTSGEVNPVHIFTGTGVFDVVLTVYSGSGLSDKATATIKVKIPT
                     LLEIEVVEYYDEYYVPGASVRLYPTLPDWIDEKNMTAEGYTDSQGFVVFSHLGPYIHY
                     VDVWEKDHNNYMLKDEDINFIRTPEIMPNRINRFVAWVDYTGNGKGARSADRSVVIRK
                     LERRSPDKEIKDSGAAVQDWKELYDRSIKLK"
     gene            11268..13418
                     /gene="cas3"
                     /locus_tag="PLZ75_02945"
     CDS             11268..13418
                     /gene="cas3"
                     /locus_tag="PLZ75_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOS71388.1"
                     /translation="MTWIAHSANSAGSTHSLSEHLLRVAEKMRDFSVNTGFESIFYFT
                     GLLHDVGKYQPAFQKYLKEGGRRGSVPHASWGATLSTKYNHFEAAFAIDGHHKGLPDR
                     GELKSDLSEIENHAHSVFSSIQSTFLNDLNLDESDLRYKDTGLRGTDKELFIRLLFSA
                     LTDADWLDTESHFNIAVSERRYGRTLDTSFLIEKLSQEINNKNKDGIINQLRNKVREY
                     ALSKAGTEPGFFSMALPTGMGKTLTSVSWALQHAQYNDMKRIIIVLPFISIIDQTAQE
                     LKRIFGDEWVLEHHSNFNVNQETDNNIADETIQDAHYTKRLAIENWDFPVIVTTSVQF
                     FESLFGNKPTQCRKVHNIAKSVVIFDEIQTLPKEMVMPTLSMLKSVQKVMGTTFLFCT
                     ATQPAFEKYEEFDGIENIQSLVENTREVYDITRRVEYFSIENYQPVTIDKLCDKVLQQ
                     NESALCIFNTKKQTLLFFNTLKNRTTDKTYHLSTGMFPAHRKEVIRKIRISLNNNVKI
                     LVSSTQLIEAGVDFDFPTVYREIAPLESIIQSAGRCNREGKMNNPGKVYIFSLMDSGA
                     PDKQYRSLAEFANSLYKGKEHLLFDHDFYKDYYRKILKLFIDADKKKIEDARKVFNFK
                     SVAQSYRLIENNTTPVFVYCEASKTLYERIRYKPVLSREDYREMQQYSVQIYDNFLKE
                     NIDKLGKEEQGYWKWFGDYNNESGISTDSPVLIL"
     gene            13442..14152
                     /gene="cas5c"
                     /locus_tag="PLZ75_02950"
     CDS             13442..14152
                     /gene="cas5c"
                     /locus_tag="PLZ75_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOS71389.1"
                     /translation="MMDNEILRVKITGEFACFTRPDLKVERMTYPCMTPSAARGILDS
                     ILWKPEFQWWIHRIIVIKPVKFFAVKRNEIKAKQGKMPIIIEDKRVQRNSIVLKDVEY
                     IIEASIYQKTEDRKNPPKKYAEIFKRRVRKGQCWRRPYLGTREFAAEFFAADGTEQPI
                     QDVIPIGSMLFDIFYDKNGKPSPLFFYDVAVKDGILECPASENEKMMHSSHIQPELNS
                     EISSVIYDFNKYEEKNEI"
     gene            14149..>15700
                     /locus_tag="PLZ75_02955"
     CDS             14149..>15700
                     /locus_tag="PLZ75_02955"
                     /inference="COORDINATES: protein motif:HMM:NF021243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOS71390.1"
                     /translation="MIRELAEFGKRIRTGHDALKDEPISIDLIINEDGSFNSFSIIEK
                     IVRPAEAITAKKGKARLLLDKAEEVLNYVSAREIINARGDLGKASKSVEHKHRLFIEK
                     LQQYQYLELLKPVFLFYNEKKESGINVALKEFEILVGEKEREGNIAFRVKDTRLHEHA
                     IVYNAIIEQYENLQAKQLKGQQKKCSVCGNPDFPVLHKPHGMIKRVPDGQKAGCALVS
                     YNENAFESYGLKGNNNSSICTACAKNYVEGLNWLLSNGTPKLIEDRKGKIKTQFTYSN
                     RKDFGKDTAMIYWTREKEIIDDINLLDGPDVGEIDQLIKSVNSENKKSRLGIKTNQFY
                     SCTLSGAAARISVRDWIEVSLDEFRQNLVKWFQDIAIKAYGEIKYTPLFALAKSGHNT
                     NSKNDPTNSRIAAHLWNAALRNSSPPLWILTAVLKRIKFIETTEDGKTRESITPERAA
                     LIRLILNRNNKNGGYMIKEQSDSNNRLPAIVCGKIFAVMESIQRAALGKDLNAGIRDR
                     FFSFASTSP"
BASE COUNT         4336 a         3278 c         3368 g         4718 t
ORIGIN      
        1 ccgacagcac aaggagcagc atcagtattt cggtgaatac gaccgcttta tttttgttca
       61 tagtggaatt aatttttcag aacggtctga aaaaacctta taacagcaat aaaacaatat
      121 gctattcaaa aagtttgaaa gcatccttta taagggaaat agcttccgcc atctgttcct
      181 cgttaatcac gaggggagga gcgaatctga taatatgtcc gtgagtgggt ttggcaatca
      241 cgcctttgtc tttcatggca atgcagacat cccatgccgt tttgcctccc ttgggcctta
      301 ttacaacagc attcagaagt cctttgcccc ggactgtttc tatcatatcc gatttaacag
      361 ccttcatttc ttttctgaaa atctcgccca ggcggacagc attctccgac agtttttcat
      421 ttttcagaac ctcgagcgca gcaataccta cttttcctgc cagtggattt ccgccgaagg
      481 ttgagccatg ttctcccggt ttgattgtca gcatgatgtc atcatcggcc agaacggcgg
      541 aaatcggcat gaccccgccg gacaatgcct ttccgagtac cagtatgtcg ggtctcactc
      601 cttcatggtc acaggcaaga agttttcctg ttcggcagag gcctgtctga atttcatccg
      661 cgataaaaag aacattgtgc tttttgcaca gttcgtagga tttcctgaga tacccttcat
      721 cgggaaccac cacgcctgcc tcgccctgga tgggttcaac aagaaatccc gccacatcgg
      781 gatcctgaag tgcttcttca agggcatccg tatcattgta aggaataatc aggaagcccg
      841 gggtaaaggg tccgtaatcg ccggtggaat cagggtcggt cgacatggag attattgtga
      901 tggtcctgcc atggaaattt ccttcacatg caatgatttt ggctttgttc tgttcaatgc
      961 ctttcttttt gtatgcccat ttgcggcaga gcttgatagc tgtttcatca gcttcggctc
     1021 ctgaattcat ggggagtacc ttgtcatatc cgaaatacct ggttatatat tcctcatatt
     1081 caccaagcac agaactatag aatgcccttg atgtcagagt aagcctgtct gcctggtctt
     1141 tcagcgcctt aatgatttcc gggtgacaat gaccctggtt gacagctgaa taagctgaca
     1201 ggaaatcaaa atatcttttc ccttcaacat cccatacgta gggaccttca cctctttcaa
     1261 ggacaacggg gagagggtga tagttgtgtg caccatattt ttcttcccgt tcgatgtaat
     1321 cctgtgtctt cattttgttg aatattaatt gattaatctt tctgtaaaag aacctgtaat
     1381 tttactaaag gtttttattt tatccaaaaa aagtgctgca tgaatgtcag acacctgaat
     1441 cttacagccg ttcagcatga aatggttccg gtattacagg ttttcagcat gacagggctt
     1501 cgggattaca gcgttttatg ttcacagcgg tccagtgggt cagcgtttcg ggcttaaaag
     1561 gcttcaatgt tacaatattt taatctgcag caatgaaaag ttctgttatg atttttattc
     1621 atcgccggca agatccggtc ttaatctttt tgttttgctg accgactgtt catgtctcca
     1681 tttatctatt tcccgtgcat ttcccgaaag caggatatcc ggcaccttcc agcccctgaa
     1741 atcagccggt cttgtatata cgggaggtgc aagaaggtca tcctggaagg aatcggacag
     1801 ggcagactgt tcatctgaaa tggcaccggg cagcaatctt actattgcat cggtaataac
     1861 agctgccggt aattctccgc ctgaaagaac gtaatttcct atggatatct ccattgttac
     1921 aagatgatcc ctgatgcgct ggtcaacgcc cttgaaatgc cctgccagga tcattatgct
     1981 gtttttaagc gacaggcggt ttgccatttt ctgatcaaac atcattccgt ccggagatgc
     2041 ataaataatt tcatcataat ccctttcact cctgagtttt tcgatggcct tccacaccgg
     2101 ttcaatcata agtaccattc ctgcaccccc tccgaatgcg taatcatcaa cgcttctgta
     2161 tctgtccttt gcaaagtcat ggaggtttac aacattgata tctgcaactc ccttgtccct
     2221 ggcgcgtttg atgatggaga aattcaaagg gctttccagg agttccggag ataccgtaag
     2281 gatgtcaatt ctcatcatgc ggatgtttgt gtaaatatat ttatttttgc cggtaagtga
     2341 ccaattagtt ttctgttatg gaatgccggc cgggttgcgg agcctgctgc acagtaattt
     2401 cgatatcatc acccattccg ggaatgtctg aaggcaagcc tgccggtgta ccttgtatac
     2461 atctgcttga agactaccgc tgtgcgcttt acggggatcc gtcaagacct ccggtatgcc
     2521 tgaattacag ggcagaacct gaattttgcg gtaacagcag ggaagaagcg atacggatcc
     2581 tgacctcact ggaggtctga aaacctgttt ttccgccggt tttccgttac atcattttcc
     2641 tgattcatag accacctttc cgtcgactat ggtataatcc acctttgtct gcataatttc
     2701 attctccgga atggtaagga gatccctgtc gactatcacc atatcggcca ggtatccggg
     2761 tttcagcatg cctttcctgt cttccatgaa ctgagcatag gcagatcccc aggtatagta
     2821 ttttattgcc tcttccatac tgagtttctg ttcagggaac catccgtccc cttcttctcc
     2881 tttccggtcc ttgcgggtaa cggctgcata cagcccttcc atgggattga gcggctcaac
     2941 ctggtaatcc gttccgaagg caaggacggc ccctgcatcc agcaggctcc tccatgcgta
     3001 tgcgccggca gatctttcag taccgattcg tttttcacag aatctcttgt cggtaataca
     3061 gtgggtaggc tgcatggacg ggatgacccc caggcgggca aagcggggaa tatctgacgg
     3121 agtgagtgtc tgggcatgct cgtcacgatg ccggctgtca cgcatgccat tcacctctgt
     3181 tgccttttca aatgcgtcca ggacccaggt gtttcccttg tcacctattg catggacagc
     3241 tatctggaaa cccattttgt cggccgcaag caccatttct tcaaactcat cgtatttcca
     3301 cattgccagt cctgatgttc ccggttcatc atcatagggt tcatgcatca gtgcggtacc
     3361 ggaacccatt gtaccgtcta tgaaggcctt gagaaacccg aacctgatcc agttgccttc
     3421 atcgggatat ttttcttcca gttccctgta gcttctgaga atatccggat cagcggtaag
     3481 cgtccgtcct atatctatcc tgcaggtaag ctcaccagct tcctgaagtt tttcgtaagc
     3541 ttcaaaatct gcgcttcctg cattctggac acttgtcacg cccagttcac gggcatgctt
     3601 cattgccaga agataaccct gccatgtttt ctgtgcgatt tcttcatctg tcagctcctg
     3661 ttttacctct ccggtttttg caaggcccat tgctgtttcc ttaagtattc ccgtaggttc
     3721 gcctgtaacg ggatctttct gaatttcacc gccgaaaggg tcgggtgtgt tttttgttat
     3781 ccccgatttt ctcagcacat agctgttaac cagtactgaa tggccatcgg cccttgaaag
     3841 gataacagga ttgtccggcg aaaccttgtc aataagctct tttgacggcc attcccgtgt
     3901 tgtgaacatt tcatggtccc agtggccccc tctgataatt tcccctggct tcgaccctgc
     3961 gacccgagcc tttacctttt cagttatcac ggcaggatcg gtggtatagc ggagctcaat
     4021 ataatccggg tcaagcggtc cgaaatggag gtgtgcgtca ttgaatccgg gaataaccag
     4081 tctgcccgag gcatcgatta ccctcgtttc tcccggattt atgaattttc tgattttgtt
     4141 tgtttttcca gtggcactta tttttccttc cttcactgca acagcttcac aaagcggatt
     4201 tgagctgtca atcgtaagta ttttgccgtt caggataacc aggtcggctg cctcaggctg
     4261 tccgcaggaa aatgtcagta ccatgacagt cacagataac aggagcaata tacgattcat
     4321 aggtaaactg gttttggacg gaaatatatt aaaataatca ttcaatagtc tttttttact
     4381 tcattttaag aattttgtca gattaataac gcagaacata tctgaaaata aataatttcg
     4441 caggaaaatc aaatatatga atattgtact tataggctat ggccggatgg gtcatgagat
     4501 tgaagaggca gccgtaaaac gcggtcatac aatcgttctt gcaattgatg cagacaacct
     4561 ggaggatctg aatgaagaga atatgaagga agcggatgta gcgattgaat tcaccatgcc
     4621 ggaagcggca ttcggaaata tttccgcctg tctgaagatg ggtgttcctg ttgtgtcagg
     4681 caccacgggc tggcttaagg attatgacag agcccgggag ctgtgcattg aaaacgggac
     4741 atcctttctt cattcatcca atttcagcat aggtgtcagc ctgctttaca ggctcagttc
     4801 ggaactggct gagtatatga agcattacag tgagtacagt gtatccattg aagagataca
     4861 tcatgtgaag aagcttgatg ctcccagcgg tacagcgatt gcccttgcgg aagggatagc
     4921 aggccgtcat ccgggatatg cggcaggatg gtgtcccgac caggaaagaa aagagggtat
     4981 gataccggta aaatctgtca gggaaggact tgttccggga acccatatta ttacctggga
     5041 ttcggatatt gacaccatca gtctgaagca tgaggcaaaa aacaggaagg gccttgccct
     5101 cggggctgtt gtggcagccg aattcatcag taccaggaaa ggtgtgttta ccatgaagga
     5161 tgttctggga ttctgaatat attttatact tttatccgct taaataaact tttgaaatga
     5221 ataaattcct tcagaagaaa tatataatgt ttgtgatagc ggcattgata tttctgtttg
     5281 tcgttgtctg gatcggcaat ttctggcttc tcctgggatt accggtgata ttcgatattt
     5341 atgtttcgaa gaaagtgaac tggacattct ggaaaaggag ggaccgcaat aacagtgctt
     5401 ttatagaatg gcttgatgca ataatttttg cagttattgc ggtcacactc ataaacatat
     5461 ttctttttca gaattacagg ataccgaccc cttccatgga aaaatcactt ctggtcgggg
     5521 atcatctctt tgtaagcaaa cttgcatacg ggcccagaat gccaaatact ccgctggcat
     5581 ttccctttac ccagcatacc cttcccctta caaaaagcaa atcatggaca gacgttgttt
     5641 actggcccta taagaggctt gccggtctgg gtcatgtaaa gcgaaacgat cccattgtct
     5701 tcaatttccc ggcaggagat actgtggttg ttgaagaaca ggcaacgagc tactatgaaa
     5761 tagtgagacg aacggcaagg gagttgcagg ccagggatag ttacgggaat ccggagccga
     5821 aggctcttga atattatctg cctgcagccc ggaaagagat acggagcagg tatgaagtca
     5881 tttacagacc ggcggacaga agggacaatt atgtaaaaag gtgcatggca ttgccgggcg
     5941 acactcttga aatacgtgca ggggaagttt atatagacgg caggcctgtt gaggaaaaca
     6001 aaacccagca gacgacatac atagtagcga ccaatggaac aaccattaat ccgaaagcct
     6061 ttgaaagact tggcatttcc aggtctgatg tgaatatgct ttcaggtaca gcctatattc
     6121 ttcctcttac aaaaagcagt gccgaagcaa tttccaaatt tccgaatgtt ctcagtacaa
     6181 cggcagtata tgccaggaaa ggggagtatt ccccgcagat ctttccctat gatcccgttc
     6241 gtgcctggaa tgaagacaat ttcggaccat tgtggattcc gcgcaagggt gtggaagtaa
     6301 aaattgacac ttccaatatt tgcctttatg aacgtattat tgaagtttat gaagaaaatg
     6361 acctgaggac agaaaatggc aggatataca ttaacggggt tgaatcagac agttatacgt
     6421 tcaaaatgga ttattacttc atgatgggtg ataaccgcca taattctgct gattcgcgct
     6481 ggtggggctt tgtgccggag gatcatattg tgggtaaacc caagattatc tggctttcgc
     6541 tgaataagga aggaaagggt ttcaggaaaa tacgcctgaa ccgtatgttt atgaaaataa
     6601 aataaggttg atacactttt cgggtggatc aaccttatat tccgggagtt tccgtgtatt
     6661 attcctttac tttttcgact atggccctga aggcctcggg atggttcatt gccaggtcgg
     6721 caagggtctt tctgttgagg gcgatacctt ttttatcgag tttgccgatg aattcggaat
     6781 aactcatgtc gtactgcctg actcctgcat tgattcttct tatccacagt gaacgaaatt
     6841 cacctttctt cctgcgtctg tcgcggtaag cgtaacccag acccttgtac agggtatcct
     6901 tggcaacggt tatcagattt tttcttccaa gataatttcc tttcgtctgc ctgagtatct
     6961 tttttcttct ggctcttgat gctactgcat ttactgatcg tggcataatt caatttttag
     7021 aaggtcagcg ttccgtcttt cacggaatct tgaatgctga acaatcggtt aatactatat
     7081 actcttataa taaatctgtc tgctggaaat aaagtgaaaa tgcttatttt ttaaccatta
     7141 aaagcttgat attgttcata tctgccttat ctacctgtcc gaagtaggta agatttcttt
     7201 tcctcttggt tgattttttt gtaaggatat ggcttttata tgcatgttta cgctttatct
     7261 tccctgttcc ggtaaatgag aatctcttct tggctcccgg atttgttttc atttttggca
     7321 ttgttctgta aatatatatt tatgtctttt ttttcttttt tggtgaaaag gagatgatca
     7381 tcctcttgcc ttcgagcttt ggaagctggt caacctttcc atattcttca aggtcgctgg
     7441 caaagcgaag cagcagtaat tcaccctgtt ccttgaacaa tattgatctt cccttgaaga
     7501 aaacataggc tctcactttc gcaccttcct caagaaattt tatcgcgtgc ctgagcttga
     7561 aattgtaatc atgatcatcg gtattgggac caaatctgat ttctttaacg attactcgtg
     7621 cagccttggc tttgatttct ttctgcttct tcttttgttg atacagaaat ttttgataat
     7681 caatgatttt gcataccggg ggatcagcat tgggggagat ctcaacaaga tccagttcca
     7741 ttctgtctgc cagtttcagg gcatcctgaa cactcattat ttctgcatca atttcatcac
     7801 caacaaccct gaccactttg gcagtgatct tgttgttaat cctgtgagcc ggctgtaaaa
     7861 ccttgccggg gtatctttta acaggtatgg ctataactct ttcctcctga ctttagttaa
     7921 tactcatttt tctgtactct tatgctccct gcttccctcc gcgcttcccg gtacttccgg
     7981 gttttattcc gttattttca atattcatca gttctttccg gttttccttt ttcctccctg
     8041 tctctcctct gtttttcaca actctcctct gttcttccca actctcctct gttctccagt
     8101 ccttaattct ccttttctcc tcacttttct cctgaactct ccggctctcc ctacgttttc
     8161 ctaaatccac cccgctctat tcagtctttt ttgttattct cagtcctctt tgttattctc
     8221 agtccgccag ttctttactg atctccgacc ggatgaaggc gataaaatcc ccgattttca
     8281 tacttccctt gtctccttcg ccctgcttcc tgacggatac tgttccgctt tcagcttctt
     8341 tttcacctat tacaagaagg tagggtatcc gctttaattc attatccctg attttcttac
     8401 cgatcttttc gcttctgtcg tcaatcagtg tgcaaatatc ggaattattt agaatttcca
     8461 aaacatttga agcataatcg ttgaatttct cgctgatagg gaggatcacg gctttttcgg
     8521 gagacagcca caggggcagt tttcctgcag tgttttcaat aagaactgcc acaaaccttt
     8581 cgagcgaccc gaatattgcc cggtggataa gcaccgggcg gtgtttctgg ttatcgctgc
     8641 cattataggt caggtcaaat ctttccggaa ggttgtaatc gacctggatg gtacccagct
     8701 gccatttccg tcctatggca tctttcacca tgaaatccag tttcgggccg taaaaggcag
     8761 cctctccggg gacgacaacc gttttcagat tcttttccct tgcggcttca attatgtcct
     8821 gttcagccct ttcccagttt tcatcagaac cgatgtactt atccttgtct tccgggtccc
     8881 gcagtgagat ctgtgccgtg aaatcattga agtttagaat tttaaagaca tagagcacca
     8941 ggtcgatgat gcttttaaac tcttccctca gctggtcggg cctgcagaaa tgatgcgcat
     9001 catcctgggt aaagcttctt actctggtca gtccgtgaag ctcaccgctt tgctcgtatc
     9061 tgtaaactgt tccgaattcc gccattctga gaggaagttc cctgtaggag tggggagtgg
     9121 cattatagat ttcgcaatga tgcgggcaat tcattggttt aagcatgaac tgctcaccct
     9181 cttcgggagt tgatatcggc tgaaatgaac tggtcctgta tttctcaaaa tgtcctgaca
     9241 gtttatacag gtttacattt ccgatatggg gagtggtaac aattttatat cctcttttcc
     9301 tgagcagtga ggtaaggtag tttatcagat tctgtcttac gtctgagccc cttggcagcc
     9361 aaaggggaag gcccagtccg actttcgggg aaaaggtgaa aagctcaagt tcctttccca
     9421 ggcgcctgtg atctctcttt ttcgcttctt caaggaagtc aagatattcc ttaagctgtt
     9481 tctgttccgg gaatgtgatg ccatagattc ttgtcagcat tttgcgtttt tcatcacctc
     9541 tccagtaagc acctgcaaca cttgtcagct ttatggcctt aatcggttcg gtggacggga
     9601 gatggggccc gcggcagaga tccgtgaaat ttccgtttga ataaagagtt attgtaccgt
     9661 cttcgagttc gccgataagt tcggttttat attcatcacc ctttctggtg aacagttcca
     9721 tggcctcagc cttgctcact tccttccttt tgtatgtgag attcattgcg gccagttcct
     9781 tcattttatt ctcgatggcc ggcaggtccg aatcggttat ggatacgcca tcacccggat
     9841 ccacatcata gtagaaacca ttctcgatgg ccggaccgat cccgaatttt attcccgggt
     9901 acaatgattc cagggcttcg gccatcagat gtgcggagga atgccagaaa gcatgccttc
     9961 cctcatcatc ctcccatttg ttaagcctga gtgttgaatc actcctgagt ggcatggtca
    10021 gatcgcggac ttcaccattc actgtagctg agtatacggc ttttgccagt cgcggactta
    10081 tttctccggc tatttcaagg cttgtgatac cttccctgta ttcctttgaa gttccgtccg
    10141 gaaacgttat tttgatcata atgagtagcc tttttaagaa gtgtgcaaag gtaattatta
    10201 tttttttatt ttgaggcttt gcaaccttgc ttcaggcagt gccgtatata atttaaaaga
    10261 ctactattgt atggccgtta agcgttggaa acaattatag gtttgttgtg aaaattaaag
    10321 ctggcacata atttgatttt tatgtcgcat ataccaaatc atcagacaat gaaaaaaata
    10381 atttttctgc tttcattcct gccggtgttt cttttctcct gcgaaaaaac accagaagca
    10441 tattttactg ttgataaaac aggacctgaa gtaggacagg aggtacattt caaaaataaa
    10501 tcccggaatg cagtggagta tgaatgggat tttggtgacg gattcacatc cggtgaagtt
    10561 aatcctgttc acatcttcac cgggaccggt gtttttgacg tcgtccttac tgtttattca
    10621 ggttcagggc tttcagacaa ggccacagca acaattaagg ttaaaattcc aactctcctg
    10681 gaaatcgaag tagtggaata ttatgatgaa tattatgtgc cgggtgcaag tgtgaggctt
    10741 tatccgacgc tgcccgactg gattgatgaa aaaaatatga cggcagaagg ctatacggat
    10801 tcccagggct ttgtggtatt ctcacatctc ggtccctata tccattatgt ggatgtctgg
    10861 gagaaagacc ataacaacta catgttgaag gatgaggata tcaatttcat ccgcacaccg
    10921 gagatcatgc caaacaggat caaccgcttc gttgcctggg ttgattatac aggaaacggc
    10981 aagggggcac ggtcggcaga caggtccgtt gtcatccgca agctggagcg cagaagtcct
    11041 gataaggaaa ttaaagactc cggcgcagct gttcaggact ggaaggagct gtatgacagg
    11101 agtataaaat tgaaataagg tcattaaaaa ttttaagcaa gggtgcaagt cattcgaggt
    11161 tgcatccttt tctatttata cctatattag taagttctct aacggccaga aaaaacctga
    11221 gatatctcaa ttttaagatg attacgaagt aaataatatc ttaacgaatg acctggattg
    11281 cacactcagc aaatagcgca ggttcgaccc atagtttgtc tgaacatctt ttgcgggttg
    11341 ctgaaaaaat gagagatttt tctgtaaata ccggatttga aagtatcttt tattttacag
    11401 gactgttgca tgatgtagga aagtatcaac ctgcttttca gaaatatctt aaagagggag
    11461 gaagaagagg cagtgtacct catgcaagct ggggagcaac gttaagtacg aagtataatc
    11521 attttgaggc tgcttttgct atagacggac atcataaagg tttacctgac agaggagagt
    11581 tgaagtctga tctgagtgaa atagaaaatc atgcacattc agtttttagt tccatacaat
    11641 ctacatttct aaatgatcta aatcttgacg aatccgatct tcgctataaa gacacaggat
    11701 taagaggtac agataaggaa ctgtttataa gattgctttt tagtgcatta actgatgctg
    11761 actggttaga tactgaaagt cattttaaca ttgctgtctc agaaagaaga tatggaagaa
    11821 ctctcgatac aagtttttta atagaaaaac tttcacagga aatcaataac aaaaataaag
    11881 atggtataat aaaccagctt agaaataaag tcagagaata tgcactgtcc aaggcaggca
    11941 ctgaaccggg ttttttttcc atggctttac cgacaggtat gggtaagact ctgacttctg
    12001 tcagttgggc tttgcagcat gcgcaatata atgacatgaa aagaatcatt atagttttgc
    12061 catttattag tattatagat cagacagcac aggaactaaa acgaattttt ggtgatgaat
    12121 gggttctgga acatcattcg aactttaatg taaaccagga aacagataat aatattgctg
    12181 atgaaaccat tcaggatgct cactatacaa aacgattagc aattgaaaac tgggatttcc
    12241 ctgttattgt gacgacttca gtacaatttt tcgaatcgtt atttggaaat aaacctacgc
    12301 aatgccggaa agtacacaat atagccaaat ctgttgtaat atttgatgaa atacaaacat
    12361 tgccaaaaga gatggtcatg ccaacactca gcatgttgaa aagtgttcag aaggtaatgg
    12421 gaacaacatt tcttttttgt acagcaactc aacccgcgtt cgagaaatac gaagaatttg
    12481 atggaattga aaatattcaa tccctggttg aaaacaccag ggaagtatat gatatcacca
    12541 ggcgggttga atatttttca attgaaaatt atcaaccggt gaccatcgat aagttatgcg
    12601 ataaagtcct gcagcagaat gaatctgctt tgtgtatttt taatacaaag aaacaaactc
    12661 tgttattttt taatacattg aaaaacagga cgacagataa aacctaccat ttatctaccg
    12721 gtatgttccc cgctcatcgt aaagaagtaa taaggaaaat caggattagt ttaaataata
    12781 atgtaaaaat actggtatca tcaactcagt tgattgaagc aggtgtcgat ttcgactttc
    12841 caaccgttta tcgggaaata gcaccattag aatctatcat tcagtcggca gggcgatgta
    12901 atcgtgaagg gaaaatgaac aatcctggta aagtatatat tttttcatta atggattccg
    12961 gagcacctga caagcaatac cggtctttgg cggaatttgc aaactcatta tacaaaggaa
    13021 aagagcatct tctctttgat cacgattttt ataaggatta ttacagaaaa atattaaaat
    13081 tgtttattga tgccgataaa aagaaaattg aagatgctag aaaagtgttt aattttaagt
    13141 cagtagcaca atcctatcgg ttaatcgaga ataacacaac acctgttttt gtttattgcg
    13201 aagcaagcaa aaccttatac gaaagaatca gatataaacc tgttctctca cgtgaagatt
    13261 atcgtgaaat gcagcaatat tcggttcaga tttatgataa tttcctgaaa gaaaatattg
    13321 ataaacttgg taaagaagaa cagggttatt ggaaatggtt tggtgattat aataatgaat
    13381 caggcatttc aactgatagt ccggtactga ttttataatt aattatataa cagataattg
    13441 tatgatggat aacgaaattt tacgtgtaaa gatcaccggg gaatttgcct gttttacacg
    13501 acctgatctg aaagtggaaa ggatgaccta tccctgcatg actccgtccg cagcaagagg
    13561 cattctggat tctattctct ggaaacctga atttcaatgg tggatacacc ggattatcgt
    13621 cattaagccg gttaagtttt tcgcagttaa acgaaatgaa attaaagcaa aacaaggcaa
    13681 gatgccaata ataattgaag ataaaagagt tcaacgtaat agcatcgttc ttaaagatgt
    13741 cgaatatatt attgaggcat caatttatca aaaaacagaa gaccgcaaaa acccacccaa
    13801 aaaatatgct gaaatattca aacgcagggt acgaaaagga caatgttggc gaaggccata
    13861 tctgggaacg agggaatttg cagctgagtt ttttgcagcg gacggtacgg aacagccaat
    13921 tcaggacgta attccaatcg gcagcatgct attcgatatt ttttatgata aaaatgggaa
    13981 accatctccc ttattctttt atgatgttgc tgttaaagat ggaattttgg aatgccctgc
    14041 ttcggaaaat gaaaaaatga tgcattcttc acatattcaa ccggagctga atagtgaaat
    14101 atcatctgta atttatgatt ttaataaata tgaggaaaag aatgaaatat gatcagggaa
    14161 ttagctgaat ttggaaagag aatcagaaca ggacatgatg ccttgaaaga tgaaccaata
    14221 tcaatagatc taataataaa tgaagatggt tcatttaatt cgttttctat aatagaaaaa
    14281 atcgtcagac ccgccgaagc aataactgca aaaaaaggaa aagcacgttt gcttcttgac
    14341 aaagctgaag aagtgcttaa ctatgtgtct gcaagggaaa ttataaatgc tagaggggat
    14401 ctaggcaaag cttctaaatc agtcgaacac aaacacaggt tatttattga aaaattgcaa
    14461 caatatcaat atttagaatt acttaaaccc gtttttctct tttataatga gaaaaaggaa
    14521 tcaggaatta atgtcgcttt aaaggaattt gaaatactag taggagaaaa agaaagagaa
    14581 gggaatattg cattcagggt aaaggataca cgacttcatg aacatgcgat agtatataat
    14641 gccattattg aacaatatga gaatttacag gcaaagcagc ttaaaggaca gcaaaagaaa
    14701 tgctctgttt gtgggaatcc tgattttcct gttttacata agccacacgg catgataaag
    14761 cgggttccgg atggtcaaaa agcaggttgt gctttagttt catataatga gaatgccttt
    14821 gagtcatacg ggttgaaagg gaataataac tcttcaatat gcacggcctg tgccaaaaat
    14881 tatgttgaag gtttaaactg gttgttatcc aatggaactc caaaactaat tgaagataga
    14941 aaaggaaaaa tcaaaactca atttacgtat tcaaaccgta aggattttgg taaagacaca
    15001 gccatgattt actggacaag ggaaaaagaa attatagatg atatcaatct tctcgatggt
    15061 ccggatgttg gcgaaataga tcaactaata aaatctgtga acagtgaaaa taaaaaatct
    15121 agattaggta ttaaaacaaa ccagttttat tcctgtactt tgtcgggtgc agcagcacgt
    15181 atttcagttc gtgactggat tgaagtaagc cttgatgaat tcagacagaa tctggtaaaa
    15241 tggtttcagg atattgccat caaggcttat ggtgaaataa aatatacacc tttgtttgcc
    15301 ttggcaaaat ccggacataa taccaattct aaaaatgatc caacaaattc gagaattgca
    15361 gcccatctat ggaatgcggc tttaagaaat tcaagccctc cattatggat tctgacagct
    15421 gtattaaaac gtataaaatt tattgaaact actgaagatg gcaaaaccag ggagtctatt
    15481 acacccgaga gggctgcctt gattcgttta atattaaaca gaaacaataa aaatggaggt
    15541 tatatgatta aagaacagag tgattcaaac aacagactac cggccattgt ttgtggtaaa
    15601 atctttgccg tcatggagag tattcaacgt gcggcactgg gaaaagacct gaatgccggg
    15661 atacgtgacc gttttttctc ttttgcatca acaagtccag
//
LOCUS       DAOSPX010000033        15594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3890_length_15594_cov_4.951026, whole genome shotgun sequence.
ACCESSION   DAOSPX010000033 DAOSPX010000000
VERSION     DAOSPX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15594
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3890_length_15594_cov_4.951026"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2031
                     /locus_tag="PLZ75_02960"
     CDS             <1..2031
                     /locus_tag="PLZ75_02960"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOS71391.1"
                     /translation="QVSTDNGINWDDIYDNAHYAGTTTQQLSIIDINYDDFHNNRYRL
                     ALKAFCATAYTNAAVLTVNRLPVVSFTDDPIPACGDVPLVITPVITGGTAPWKSHSWT
                     GDIGPLNNYFSPAPTFRTRIAGTYDLTYRVVDDNMCAGEGSVSVVVDAPDATFTQDIS
                     MACTPATVKFSKDMTGMKSWEWNFGDGTPVNTTDENPVHVFTNTTSTAIVYRTIKLTV
                     ESMAGCYDTKTSMMTVYPAVIAHLTASDDVVCHGNQIVFTADPGANIYMWDFGDGTPR
                     VPGVNTSTHMFTNLTGTPVTRTVKVVTTSMYGCSDSTTMDIVVMPMPLAQFSATPNNQ
                     TYDAVGNKVVFTDETTPVSTWTYHWDFGDGGSSTLQHPEHTFTGTGTYNVVLTVTNGK
                     CTSTISHPVHILPLPPVADFDSIPSGCATWTITPNNTSLNTEMPGTTYRWDFGDGSYS
                     TAKNPSYTYFKAGEYRVELTVTGPGGMSVKSQVVRAYPSPRAYFEVSTTLVFVNDERV
                     RCFNLTEGGDYFVWEFGDGDTSRMREPYHKYMEEGVYDITLWAYNNDGCRDVFVLSPG
                     VTVQPAGDVRFSTVFRPNPTGPIERSDLPTGGNEIDQFFFPPIRDKVIKYKLQVFNRL
                     GVLIFESHDINVPWNGYYKGQLCPQGVYVWYVEGKYANGQPFKKVGDITLLH"
     gene            2376..3476
                     /locus_tag="PLZ75_02965"
     CDS             2376..3476
                     /locus_tag="PLZ75_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS71392.1"
                     /translation="MTENPFKFGTVVDGIYFTDREEEVSQISSYLKSENHLIIISPRR
                     FGKTSLIRKILSASGRRFIYLDMQLVLTEEDFAAQLLKRIYRIFPVQKLKNYIKSFRI
                     IPTVIMNPVTGDVEISFSPGSRDLTPLEDVLNLVEKLGKPDDKIIVVLDEFQDVFRIN
                     TGLVRMLRSVIQVHKNINYVFMGSSESMIREIFEKKDSPFYRFGILYPLGKIPETKFR
                     LFLEYNFQGIADDPATVSGEILKITGAHPYYTQQLAFMVWELMNRKGLEQGMLADSAA
                     DEIVRSHDNDFELLWNTLNRTDMMVLAGMAESDVSPLSDEFRKQFGTGAASTVYSTLQ
                     RLVKKGMVIRDDTGYFIDDPFFRRWIRMRRRV"
     gene            3901..4086
                     /locus_tag="PLZ75_02970"
     CDS             3901..4086
                     /locus_tag="PLZ75_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71393.1"
                     /translation="MSWERVTACGKDQIVRIAVSIIPERLSDEDFSVHLLLPGKEGRL
                     SDEDFSVLFLLPGKEED"
     gene            4283..5524
                     /locus_tag="PLZ75_02975"
     CDS             4283..5524
                     /locus_tag="PLZ75_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOS71394.1"
                     /translation="MKRNIKLFLAAMAVVILLVTCSKVDNGEPDLTPKSISLTAVASQ
                     AIQSSNEFGIGLFTKVAETERDNLMLSPLSASTALTMLLNGCGGDTYSQLQETLRYPS
                     GMTTAEINEAYKSLVDQLLKADPKVKLALANAIFYRSGFIVKQPFLATMSKDYKATVE
                     ALNFASPAALNTINKWAGDNTNGKIPKVLDEIDANAVMFIMNALYFKGSWSYRFDKTG
                     TMDRLFYPDGGSAVYVSTMKGEVGAKVVSGNDYRAVEMPYGRSNFTMVVIVPTATLAE
                     FNSTFTNLVWNSITGAFDNTDEFEKLFVYMPKFKFSYEKYLNDQLKSMGMVDAFLAEK
                     ADLSGISDQGIFVDFVKQNTFVEVDEEGTEAAAVTTIGIRDSFNPEPREFIVDKSFIF
                     AIRERTTNTLLFIGQVVKPEV"
     gene            complement(5641..5985)
                     /locus_tag="PLZ75_02980"
     CDS             complement(5641..5985)
                     /locus_tag="PLZ75_02980"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOS71395.1"
                     /translation="MKLLLDQNISFRITNKIQDIFPESKQVKDLGLENSKDSAIWTFA
                     KENDYCIVTFDADFYDLGLIKESSPKVIWLRLGNTSTQEIEKILRKNFDLIKTFLTDP
                     DYKEIGCLEINF"
     gene            complement(5985..6206)
                     /locus_tag="PLZ75_02985"
     CDS             complement(5985..6206)
                     /locus_tag="PLZ75_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOS71396.1"
                     /translation="MKAYNKIIKINPDKRFGKPCIRNTRIAVFDVLGWLGSGMTYDDV
                     LSDYPELTREDIRATLMFAADRERKLQTI"
     gene            6430..7410
                     /gene="pfkA"
                     /locus_tag="PLZ75_02990"
     CDS             6430..7410
                     /gene="pfkA"
                     /locus_tag="PLZ75_02990"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOS71397.1"
                     /translation="MGKIKRIGVLTSGGDAPGMNAAIRSVVRTAVYNGLKVTGIRQGF
                     YGMIYKDFVEMVASSVSDIIQRGGTILKTARCDEFMTEEGRLTAYRNLREAGIDGVVV
                     IGGDGSFIGARLFCEEYDIPFVGIPGTIDNDICGTDYTIGYDTALNTVVQAVDKIRDT
                     ADAHNRIFFVEVMGAEAGYIALYGGLASGAEAILIPETTDEERDLIKIIRPDSGRKKT
                     SMIVIVAEGDESGGAIALSEKVKSEFKDLDIRVSILGHLQRGGSPSATDRVNASRMGH
                     AAVEALLDNQKSMMIGLVNNEITLISFRRSLKLNKHINHKLIELAKILSA"
     gene            complement(7638..7955)
                     /locus_tag="PLZ75_02995"
     CDS             complement(7638..7955)
                     /locus_tag="PLZ75_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71398.1"
                     /translation="MKTLKILTRLLTGMLFVHKNAEHKAIVEKTKNFLPGFYSPFGLK
                     LLSTIDFIITEKNIETPEAITKELANWSNRKKTMFTNPNFIQIATKNIKQHNLPTANK
                     GLA"
     gene            complement(8198..10528)
                     /locus_tag="PLZ75_03000"
     CDS             complement(8198..10528)
                     /locus_tag="PLZ75_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOS71399.1"
                     /translation="MKKLIILSGFILLIISGCKSRQMQNTEYGSFLYDHKAMPMDTSN
                     SLQYKWNHKEILESVLIDGMESLENWELTFGNRENVASISLTDEKVFEGKSSIKFISP
                     TKLPVSTGADGRYWGRQNLTRKFPAEDFSEYNRISINIYPEFKGFRKLYLTLILHNED
                     NVPDQYGKEGWHTVMLENNRWNRLVMEIPHLPHDKVTGITISYGLQGNEPDAADTIVY
                     YADNLRLEVVEPDYYEGWGTDKAISFSHSGYNIRDQKTAFTSVTGGDKFRLVNHETNK
                     TVLEKDVLKQAGRIGSFTVFDFTEIKAPGNYRIVYGDTESRPFPIGRDVWLPVIEKIT
                     NQFYLQRCGFDQPGIHLKCHTDWYTVYRGDTIVMAGGWHDAGDLSQSYWQTAGATAAF
                     FRLARAYARENKRLSERLIEEGSWGLEWLHKNRFDGLQVISWTTHDCYTDGVIGNFDD
                     KHVQPGSRINTNDNYYSIIADVEAALTLKGRDKALADKSAQYAVEYWNLLSEHPHRWN
                     TESLAIAVLAGTKLYQLTKDEGVKNLTISYADSLMTFQQKEAMNWEIPLSGFFYADRS
                     RNRFFGYNHSCTAASPVLGLVELCRLLPGDEKYAEWFRTVELHKNYLIDIAQLTAPYF
                     MIPANIYKLGGTSDDAQILNGIRLDDDHYLRLFPVWQAFRGNSSVILSPAQGLASANR
                     LFKDPVVHQVALAQLEWMQGKNPFSQSLMYGEGYDFSPQYAVFTGDITGGLPVGILTR
                     DDLDIPYWKTSVLHNYKELWGQPAFRELELIYYLKQ"
     gene            10693..10935
                     /locus_tag="PLZ75_03005"
     CDS             10693..10935
                     /locus_tag="PLZ75_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71400.1"
                     /translation="MALLKYIERVRRIDMLIHMKATGPPVEFASKLGIRRSTLFQSLQ
                     EMKEMGVDIRYSNTRETYYYADGRRIEIKVEKTTGS"
     gene            11036..11698
                     /locus_tag="PLZ75_03010"
     CDS             11036..11698
                     /locus_tag="PLZ75_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71401.1"
                     /translation="MKVLEGYFGLERNKLLFIMISVNLLLIWLSKSVLLNETVFYNTY
                     SEQLTYERSMELFKRIMDLSWVSYLFSPLVLIIKFSVLSLIIYTGFFLSDLHGKVSLG
                     MIFTTVVASEIIIVFASLAKFLWFTFFAGNYTLDDMNFFYPLSLINLFKKGEVAQYWI
                     YPLQSVNLFQVAYILMLATGLRTISSVKREKTDTIVLLTYGTALILWIAVIMFITIDI
                     QS"
     gene            11695..12228
                     /locus_tag="PLZ75_03015"
     CDS             11695..12228
                     /locus_tag="PLZ75_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOS71402.1"
                     /translation="MKKILTFLITVSVCAILFILAKGIFLKVLKANDRDDRIKTLPSF
                     SLGTLNGEIFNSADLKNGPLLLVYFHPDCDYCQYEISSLIRHDIQSKGVKVLLVSNAL
                     PETIDEFMEQFETGQDEFCILSDSSLVFANLFGISVIPVSLIYNENLELIKFFKGEVS
                     PNAILNFLNDVCQYQEN"
     gene            12200..14374
                     /locus_tag="PLZ75_03020"
     CDS             12200..14374
                     /locus_tag="PLZ75_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOS71403.1"
                     /translation="MMSVNIRKIKEKALLQHDSTDCGAACLASAIRYFGGESGIERIR
                     KLSGTSQSGTTMLGLYQAAVASGLKAQGYEASVESIKSFKGLLILHVSPEIRYDHYIL
                     SFGYEDGRFVLWDPANGLTTKTAEELEKIWPGHKCLSVEPGEGFVFKKEEDKGKRKWL
                     INTIKPDRELLMISILLGLLISALGAVMAIFTQKLIDRILPSGEVKVLLISSVLVFVL
                     LISRVVLLSLRQYILLSQGKSFNIRIVDGFYRSLLGLPKLFFDTRKTGDLVARLNDTM
                     RIQRVISDLISIYFIDIVVLIVTSVLIFIYSLPAGIISVIAIPLIYLLVYRWNKRIIS
                     SQNELMTGYAMNESNFINSLRGIAEIKCMNWQGYYSLRNESVFSNFQEKFFSLGKIRV
                     SLGMLTNLAGTLYLIVLLIYSSLKVMDLQMTQGELMAILSLSSTLIPSVMNLALISIP
                     LSEAKVAIARMFEFTRISPEETDSGNIKENIAIEKISLNNMKFRFPGQKLFLTDINLE
                     IEKGQIIALVGESGSGKSTLVNILLRFLIPESGDIIINDIVPVNQIMPGVWRRSVGVV
                     PQDIHIFNGTILENLIPEPSEKEVEILLRLISDYGLESFIEALPLGLVTLVGEDGVKL
                     SGGQKQVIAFLRALINEPDILLIDEGTSGMDKDTEDLMLKMLISLKAKLGILLVTHRI
                     SLIKKICDRIYILEDRTIKESGTHYELISSENIYKRYWEELN"
BASE COUNT         4435 a         3142 c         3484 g         4533 t
ORIGIN      
        1 caggtaagta cggataacgg aattaactgg gacgatattt atgacaatgc acattacgcc
       61 ggtaccacta cacagcagtt gtcgataata gatattaatt acgatgattt ccataacaat
      121 cggtacaggc tggctctgaa ggcattctgt gcaacagcct ataccaatgc ggccgttctg
      181 acagtcaaca ggcttcctgt ggtatcattt accgatgatc ctataccggc ctgcggtgac
      241 gttccactgg tgattacccc ggtaataacc ggaggaaccg ctccctggaa atcacatagc
      301 tggacgggcg atatcggtcc gcttaacaac tatttcagtc cggcaccaac cttcagaacc
      361 cgtattgcag gaacatatga tctcacttac agggttgtgg atgacaatat gtgtgcaggc
      421 gagggcagcg tatccgtggt tgttgatgcc cctgatgcga cattcacaca ggatatatca
      481 atggcctgta ctcctgccac ggttaagttc agcaaggaca tgacaggaat gaagagctgg
      541 gaatggaact ttggcgatgg cacaccggtt aataccactg atgagaatcc ggtgcacgtg
      601 tttacaaata caacttccac agccattgta taccgtacaa ttaagctgac tgttgagtca
      661 atggcaggct gctatgacac caaaacatca atgatgactg tttatcctgc agtgattgcc
      721 catctgacag ccagtgatga tgtcgtgtgc catggtaatc agatagtatt cacagcagat
      781 cccggggcca atatttacat gtgggatttt ggtgacggaa ctcccagggt acccggagta
      841 aacacttcaa cccatatgtt caccaatctc actggtactc cggtaacccg gacagttaag
      901 gtggtaacaa catcaatgta cggatgttcc gacagtacaa cgatggatat tgttgtgatg
      961 cccatgcctc ttgcccagtt ctctgcaaca ccgaacaatc agacctatga tgctgtagga
     1021 aacaaagtgg tgttcaccga tgaaaccaca ccggtaagca cctggaccta tcactgggat
     1081 ttcggtgacg gcggatcatc aacattgcag caccctgaac atacctttac gggaacgggt
     1141 acctacaatg tggttctgac tgttacaaac ggcaagtgta ccagcactat aagtcatccg
     1201 gttcatatat tgcctttacc tccggtagcg gacttcgatt caataccttc gggatgtgcc
     1261 acctggacca tcacgccgaa caacacttca ctcaacaccg aaatgccggg aacaacctac
     1321 aggtgggact ttggtgacgg aagctattca acggcaaaga atccgtccta tacctacttc
     1381 aaggcaggag aataccgtgt tgaacttaca gtaaccggtc ccggcggaat gtcggtgaaa
     1441 tcgcaggttg taagggccta tccttctccg agagcctatt tcgaggtaag taccacactt
     1501 gtattcgtaa atgatgaaag agtcaggtgc ttcaacctga ccgagggcgg ggattacttt
     1561 gtatgggaat tcggagacgg agatacttca cggatgagag aaccatatca caaatacatg
     1621 gaagaaggag tctatgacat aaccctgtgg gcttacaaca acgacggttg cagggacgtg
     1681 ttcgtactgt cacccggagt gaccgtgcag cctgcgggag atgtaagatt ctccacggtc
     1741 ttcaggccca atcctacagg accgatagaa aggagcgatc tgcctaccgg aggcaatgag
     1801 atcgaccagt tcttcttccc gcccatcagg gacaaggtga tcaagtataa gcttcaggtc
     1861 ttcaacaggc ttggtgtgct gatattcgaa agtcacgaca ttaatgtacc gtggaacgga
     1921 tattacaagg gtcagctatg cccgcaggga gtgtatgtgt ggtacgttga gggcaaatat
     1981 gcaaacggtc agccattcaa gaaagttgga gatataacac tgctgcatta acaagatccg
     2041 gggttggtgg ctgaaggccc gaaccccggc ttacaaattg aatcagacaa aacggcccgt
     2101 cgcaggacgg gctttttttt gcatgccctg tccggattgc ttttaggatt acgaaaatta
     2161 tcataaattt gattgcgaaa attatcgtaa cgattaatca cgtaatggaa aatatcacaa
     2221 taaaaaatct ctcattcagg aatctcgtaa aaggcatacc ccctacgtaa ctttgtttct
     2281 ttgatgccgg atttgctttt aaaaatcctg tcagcggaat atccgcaaaa aggaatcaaa
     2341 tattcgtaaa cataataaga aataacaaca gggaaatgac agaaaacccg ttcaaattcg
     2401 gaactgtagt tgatggaatt tattttaccg acagagagga ggaagtatcg caaatatctt
     2461 cctacctgaa aagtgagaac cacctgataa ttatcagtcc aaggcgtttc ggcaagacaa
     2521 gcctgataag gaaaatactg tccgcctccg gccgccgctt catatatctg gatatgcagc
     2581 ttgtgcttac ggaggaggat ttcgcggcac agctgcttaa aaggatctac aggatcttcc
     2641 ccgtgcagaa gctgaagaac tatatcaaat ccttccgtat tattccaacc gtcataatga
     2701 atcccgtgac gggagatgtg gaaatatcct tttccccggg ctcccgggac ctgactcccc
     2761 ttgaggatgt cctgaacctt gtggagaagc ttggaaaacc tgatgacaag ataattgtag
     2821 tgctcgatga gtttcaggat gttttcagaa tcaataccgg acttgtcagg atgctgcgct
     2881 ccgtgataca ggttcataaa aatatcaatt atgtctttat ggggagcagt gagtcgatga
     2941 tacgggagat ctttgaaaag aaagattcac ccttctacag gtttggtata ctatatccgc
     3001 tggggaagat acctgaaacg aaattccgtt tgtttcttga atataatttc caggggattg
     3061 ctgatgaccc tgcgacagta tccggagaaa tacttaaaat aacaggggca catccgtact
     3121 atacccagca gcttgccttt atggtatggg aactgatgaa ccgcaaggga ctggaacagg
     3181 ggatgcttgc cgattcagcc gcggatgaaa ttgtaagaag ccatgacaac gactttgaac
     3241 tgctctggaa cacgcttaac cggacagata tgatggtact tgcgggaatg gctgaaagcg
     3301 atgtttcgcc tctgtcggat gaattcagga aacagttcgg aacaggagcc gccagcacgg
     3361 tatacagcac tctccagagg cttgtaaaaa aaggaatggt tatcagggat gatacgggtt
     3421 atttcatcga tgatcccttc ttcagaagat ggatcaggat gaggagaagg gtttagcttc
     3481 ctatttcatg attccggatg aaaatcataa tttagtgaat catacaatgg gtcgttaaac
     3541 ttttgagtct gttgattccc ggcaggagta tttatgtgat aacgtattgc tgcctggtta
     3601 cttattgaaa tataacaaac tgttattata acaataagca attcaaaaag cctggtgaag
     3661 aaatacaaaa aagatactct gacagagata ctgcttatga gatattttta ctgatgatga
     3721 acaataataa cttcaaagat tatttccgta aagcttcact ctggaagatc ggactgatct
     3781 ggggcctgat tacgggaatc ctggtgtcta tagttcagga taagactgtt ctgaaatatt
     3841 ttctcagccg ggaaatagtt ttgcaattgt tgttttttat gattgccgga gccgggctgt
     3901 ttgtcctggg aacgagttac cgcctgtgga aaagatcaga tagtaaggat agcggtttca
     3961 ataattccgg aaagactgag tgatgaggac ttttcagtac acctccttct tccgggaaag
     4021 gagggaaggc tgagtgatga ggacttttca gtactctttc ttcttccggg aaaggaggaa
     4081 gactgaaaac ccaggttagc aggatttgct gcaggcttca acgtgtcatt acagaaatta
     4141 cacttttacc cctgagaatt tggcacccgg tcattgacgc ttacatgacc gggggaaaaa
     4201 aacggcagtg cactgcagca ttttgaaggt ttcatgcgta atagtaatat aaacgcggac
     4261 aacattaaaa aagaataatc atatgaagcg gaatattaaa cttttcctgg cagccatggc
     4321 tgtagttatc cttctggtga catgttcaaa ggttgacaac ggtgaacctg atctgacccc
     4381 aaagagtatc agtctgacag ctgtggcttc acaggccatt cagagcagca atgaattcgg
     4441 gatcggactt ttcacgaagg ttgcggaaac tgaaagggat aatcttatgt tatcgcccct
     4501 gagtgcaagc acggccctca cgatgctgct aaacggatgc ggcggtgata cttattcaca
     4561 gctccaggaa acactcagat atccgtccgg tatgactacc gctgagatta acgaagcata
     4621 taagagtctt gttgaccagt tgctgaaagc tgatccgaaa gtgaagcttg ctcttgcaaa
     4681 tgcaattttc taccggagcg gattcattgt aaaacaaccg tttcttgcca caatgagcaa
     4741 ggattacaaa gccacggttg aagctctcaa cttcgcgtca ccagctgcgc tgaatacaat
     4801 caataaatgg gccggcgaca ataccaatgg taaaattcca aaggtactgg atgagattga
     4861 tgccaatgcg gtaatgttta ttatgaacgc actctatttt aagggcagct ggagttaccg
     4921 tttcgacaag acgggaacca tggatcgtct cttttacccc gacgggggtt cagcggtgta
     4981 tgtaagcacc atgaagggcg aagtgggagc gaaagtagta tctggtaacg attacagggc
     5041 agttgagatg ccctacggac gatccaattt cacaatggtt gtgattgttc caacggcaac
     5101 actggcggag ttcaattcca ccttcacgaa tctggtatgg aactcgataa ccggagcatt
     5161 cgacaataca gatgaatttg agaagctttt tgtctatatg cccaaattca aattctcata
     5221 tgagaaatac ctgaatgacc agttgaaaag catgggtatg gttgatgcat tccttgcaga
     5281 aaaagcagat ctttcaggaa tttcagatca ggggattttt gtggattttg taaagcagaa
     5341 cacattcgtt gaggtggatg aggagggaac ggaagccgct gcagttacaa cgataggcat
     5401 acgtgacagt tttaatcccg aaccccggga attcattgtc gacaagtcat tcatatttgc
     5461 catacgtgaa cgtacgacaa ataccctgct gtttatcggg caggtggtga agccggaggt
     5521 gtaaaagtca aatgatcaaa acgactgaga tttaggtatt gcgcttaaaa gttgcggaca
     5581 aacctttaaa tgattcttct caaatgctgc aatcactctg attcttttag caaagtgatt
     5641 ttaaaaattt atctcgagac agccgatctc cttataatca gggtcagtta agaatgtttt
     5701 tatcaggtca aaattttttc gaagaatttt ttcaatttcc tgagtactcg tgtttccaag
     5761 tctcagccag attacttttg gagaagattc ttttatcaaa cctaagtcgt aaaaatcagc
     5821 atcaaaagtg acgatgcaat aatcattttc ttttgcaaag gtccatatag ctgagtcttt
     5881 ggaattctct aatcccaaat cttttacctg ttttgattca ggaaatatat cttggatttt
     5941 atttgtaatt cgaaaggaaa tattttgatc aagaagtaat ttcatcagat tgtctgaagt
     6001 ttacgttctc tatcggccgc aaacataaga gttgccctaa tgtcttcccg ggtaagttct
     6061 ggataatctg aaagaacatc gtcatatgtc atacctgatc caagccaccc cagaacatca
     6121 aaaaccgcaa tcctcgtatt tcttatacaa ggttttccga atcttttgtc agggtttatt
     6181 tttattatct tattgtaagc tttcatgatg caaagttaat ctttttcatt ttaatcctgt
     6241 tgggacgggt atttcatttt gcctaacttg ttataaaaac aacagatatt tttaatactc
     6301 acatgtacgg gtaaatatcg ttatggctgt taactatttc agattaatgt actcgtcagt
     6361 attccccggg gttgatccat ttttaaatat ctgttacctt tgggcgactc ttataaagaa
     6421 cagagatata tggggaaaat taaaagaatc ggagtgctga catcgggagg agatgctccg
     6481 ggcatgaatg ccgcaataag atcagtggtc agaactgcgg tttacaacgg acttaaagtt
     6541 acaggtatca ggcagggatt ctacggaatg atatataaag atttcgtcga aatggtggct
     6601 tcatctgtaa gcgatataat tcagcgcggc gggacaatcc taaaaacggc gcgatgtgat
     6661 gagtttatga cagaagaagg aaggcttact gcttacagga atctgagaga agcaggtatc
     6721 gacggtgttg tggtaatagg cggggatggt tccttcatcg gcgccaggtt attctgcgag
     6781 gagtacgata tcccttttgt aggtattccg ggaacgatag acaatgacat ctgtggtacc
     6841 gattacacaa taggttatga tactgctctg aatactgtcg tgcaggcagt cgataagatc
     6901 agggacacgg cagatgctca caaccgtata tttttcgttg aggttatggg tgctgaagca
     6961 ggttatattg cactttacgg tggactggcc agcggggcgg aagctatact gattcctgaa
     7021 accacagatg aagaacgtga tctcattaag atcatcaggc ctgatagcgg tagaaaaaaa
     7081 acaagcatga tcgtgattgt ggcagaaggt gatgagagcg gcggcgccat tgcactttcc
     7141 gaaaaagtga agagtgaatt caaagatctg gatataagag tgtcgatact cggtcatctg
     7201 cagagaggcg gatctccttc ggcaaccgac agggttaatg caagcaggat gggacatgcg
     7261 gccgtggagg ctcttcttga caatcagaag agcatgatga tcggccttgt aaacaacgag
     7321 atcactctta tttcattcag aagatcgtta aagcttaata agcatataaa ccacaagctg
     7381 atagagcttg caaaaatatt atcggcttaa tggctcaacc gcaaaagcac agtgaccagc
     7441 tggacggtcg ttatcaaatg gataacctga ctgaaattac tatacgacct cttgattagg
     7501 acggaaaata atatgttctg tggtaatcat cgcttgtccc gctttaacgg gaatgcatca
     7561 gagaatgcag caatcatgta ctcgctgctt gacatagttg gtttttgtgc ggttggcatt
     7621 gagcgttggc aagccaatta cgccaaaccc ttgttagcag taggcagatt gtgttgttta
     7681 atattttttg ttgcaatttg aatgaagttt ggattagtga acatcgtctt tttacgatta
     7741 ctccaatttg ccaattcctt agtaatagct tcaggtgttt cgatattctt ttcagtaata
     7801 ataaagtcaa ttgttgaaag tagtttcaat ccaaaaggag aataaaatcc aggcaagaag
     7861 tttttggttt tctcaactat agctttgtgt tctgcattct tgtgtacgaa cagcattcca
     7921 gtcaataatc gtgtcaatat tttcaatgtc ttcattagtg tattgtgttg agaaactatc
     7981 aactgcgtgt ccatctgtaa aaacaaagtt acagttattt ttagtcaggg ctatgttcac
     8041 atgatatttt gttttctctt tcggtgtatt tctatgtaag atttaaaagt ttctatctag
     8101 atgtgggtta cactgcttat tgccagcttg ttgcaaatgg gtggatttcg ttatgcctgt
     8161 taaccatttc agattatcat gcttgttggg gcttctctta ttgtttcagg taataaatca
     8221 gttccagttc ccggaatgcg ggctgccccc acagctcttt ataattatgc aggacacttg
     8281 ttttccagta cgggatatcc agatcgtcac gggtgaggat cccgacaggc agtccgcctg
     8341 ttatatctcc tgtgaaaaca gcatactgcg gtgagaaatc atatccttct ccgtacataa
     8401 gactctggct gaatggattt ttcccctgca tccactccag ctgggccagg gcaacctggt
     8461 gtaccacagg gtctttaaag agccggtttg cggatgccag tccctgagcc ggtgagagga
     8521 tgacagaact gtttccccgg aaagcttgcc ataccggaaa caacctaagg taatgatcat
     8581 catccagcct tataccgttg agtatctgtg catcatccga agttccgcca agtttgtaga
     8641 tgtttgccgg aatcatgaaa tacggagctg taagctgtgc aatatcaata agataattct
     8701 tatgtaactc taccgttctg aaccattcag catacttttc atccccgggt aacagcctgc
     8761 aaagttcaac gagaccaagt accggtgagg ctgccgtaca actgtgatta tacccgaaga
     8821 acctgttccg ggatctatcc gcataaaaga agccgctaag gggtatttcc cagttcatcg
     8881 cttccttctg ctggaaggtc ataagagaat cagcatagct gatcgtcagg tttttaactc
     8941 cttcatcctt tgtcagctgg taaagctttg ttccggccag aacagctatt gcaagacttt
     9001 cagtattcca cctgtgagga tgctccgaca ggagattcca gtattccacg gcgtattgcg
     9061 ctgacttatc agccagggcc ttatctctcc ctttaagggt aagagctgct tcaacatcag
     9121 caattattga ataataatta tcatttgtgt ttatcctgct tcccggctga acatgtttgt
     9181 catcgaaatt tcctatcaca ccgtcagtgt agcagtcgtg ggttgtccag ctgatgacct
     9241 gaaggccgtc gaagcggttt ttatgaagcc attcgagtcc ccagctgcct tcctcgatca
     9301 agcgctccga aagccttttg ttttccctgg catatgcacg ggcaagcctg aaaaaggccg
     9361 ccgtggcacc ggctgtctgc cagtatgact gggaaagatc acccgcatca tgccatccac
     9421 ctgccatgac tatcgtatct cccctgtaaa cggtatacca atcggtatgg catttcagat
     9481 gaattcccgg ctgatcaaac ccgcatctct gaaggtagaa ctggttagtg atcttttcaa
     9541 taactggaag ccatacatcc cggcctatcg ggaaaggtct tgattccgta tcaccatata
     9601 caatcctgta gttccccggg gcttttatct ctgtgaaatc aaataccgta aatgatccga
     9661 tcctgcctgc ctgtttgagc acatcctttt ccagaactgt tttgtttgtc tcatggttaa
     9721 caaggcgaaa cttatctcct cctgtaactg aagtaaaggc agtcttttga tccctgatgt
     9781 tatatcccga gtgcgaaaaa gatattgcct tgtctgttcc ccaaccttca taataatcag
     9841 gctcaaccac ttcaagtctg aggttatctg cataatagac aatagtatcg gccgcgtcag
     9901 gttcatttcc ctgcaaaccg tagctgatag ttatcccggt cactttgtcg tggggcaggt
     9961 gggggatttc catcacgagc ctgttccacc tgttgttttc gagcattacc gtgtgccagc
    10021 cttcctttcc atactgatcg ggtacattgt cttcgttatg cagtatcagt gtcaggtata
    10081 atttcctgaa tcctttgaat tcgggatata tattgattga aatccggtta tattctgaaa
    10141 aatcctcagc agggaatttg cgtgtgagat tttgtctgcc ccagtacctt ccgtcggcac
    10201 cggtgctgac aggcagtttt gtaggagata taaatttaat cgaagacttt ccctcaaaaa
    10261 ccttttcatc cgtcagactg atgcttgcaa cattttcccg gttaccgaaa gtgagctccc
    10321 agttttccag cgattccatt ccatcaataa gaacggattc caggatttcc ttatgattcc
    10381 atttatactg caggctgttg gaagtatcca ttggcatggc tttatggtca tacagaaagg
    10441 aaccatattc cgtgttttgc atctgtctgc tcttacagcc ggaaattatc agcaggatga
    10501 agcctgaaag gattattaat tttttcatgg tttattaaaa tcaccattca attcacaaca
    10561 tatcaaaatt actgctaatc tttcacatac aggtgaaata aataatcata aaaattttat
    10621 tgagtttctc tggactcata taatatattt atctatattt gatcagctgc gtttctttaa
    10681 aaccaaaata tcatggctct cttaaagtat atcgaaaggg tgcgccgtat tgatatgctt
    10741 atacatatga aggcaacagg cccgccggtg gaatttgcca gcaagctcgg cataaggcgg
    10801 agtaccctgt ttcaatccct tcaggagatg aaggaaatgg gagttgacat cagatattcg
    10861 aatacaaggg agacatatta ttatgcggat ggaaggagaa ttgaaataaa agttgaaaag
    10921 acaaccggca gctgatcgta gtgtttggtt tttctgactc ttagggtatt ttgacaaatg
    10981 ctctatgatt gtacgacaac aagttacaat aaataatatt tcaattcgct gaacaatgaa
    11041 agtgctggaa ggatacttcg gcttggaaag aaacaagctg ttatttataa tgatatcagt
    11101 taatctgttg ctgatatggt tgtcgaaaag cgtactctta aacgaaactg tattttacaa
    11161 tacctacagt gaacagctga cctatgaaag gtccatggaa ctttttaaaa ggatcatgga
    11221 cttatcctgg gtcagctatc tgttttcccc gcttgtcctg atcatcaaat tttcggttct
    11281 gagcctgata atttatacag gattcttcct gagtgacctg cacggaaaag tgtcactggg
    11341 catgatcttt accacagttg ttgcttcgga aattatcatc gtgtttgctt cccttgccaa
    11401 atttctgtgg tttacatttt ttgccggtaa ttacacactt gacgatatga atttcttcta
    11461 tcccctttct cttattaatc tgtttaagaa aggtgaagtg gctcagtact ggatctaccc
    11521 ccttcagtcg gttaatctgt tccaggtggc ttacatactg atgctggcca caggcctgcg
    11581 gacaatcagt tctgttaaaa gggaaaagac cgatactata gtgcttctga cctatggcac
    11641 ggccctgatt ttgtggattg cagtgataat gttcattacc attgatattc agtcatgaaa
    11701 aagatattga cctttttgat aacagtatct gtctgtgcta ttcttttcat cctggcaaag
    11761 gggattttcc tgaaagtgct aaaagcaaat gaccgtgacg acaggattaa aacgctgcct
    11821 tccttttcac tgggaacgct gaatggcgag atttttaatt cggcagatct taaaaatggt
    11881 cctttgcttc ttgtttattt ccatcctgac tgtgattatt gtcaatatga gatatcgtct
    11941 cttatccggc acgatattca gtcaaagggt gttaaagttc tgcttgtcag caatgccctg
    12001 ccggaaacta ttgatgaatt catggaacaa tttgaaacag gacaggatga attctgtatt
    12061 ttatcagaca gctcccttgt ttttgcaaac ttgtttggta tcagtgttat ccctgtcagt
    12121 ctgatatata atgaaaatct ggagctgatt aaattcttca agggagaagt tagccccaat
    12181 gcaattttaa attttttgaa tgatgtctgt caatatcagg aaaattaaag aaaaggctct
    12241 gttgcagcat gattccactg actgcggagc tgcctgcctg gcttcggcca taagatattt
    12301 cggcggggaa tcaggcattg aaagaataag gaaacttagc ggaacatcac aatcgggaac
    12361 cacaatgctc ggactttacc aggcagctgt tgcaagcgga ttaaaggcac agggatatga
    12421 ggcttcagtt gagagcatca agtcatttaa gggattattg attcttcatg tgagtccgga
    12481 aataagatat gatcattata tattaagttt tggatatgaa gatggccgtt ttgtgttgtg
    12541 ggatcctgcg aacggactga ctacaaaaac agctgaagaa ctggaaaaga tatggcccgg
    12601 acacaaatgt ctttcagttg agcccggaga aggttttgta tttaaaaagg aagaagataa
    12661 agggaagcgc aaatggctga tcaacaccat aaaacccgac agggagcttt taatgatcag
    12721 tattttactg ggcttgctca tatcggcact gggcgctgta atggccattt tcacacagaa
    12781 gctgattgac cgtattttgc cttcgggtga agtgaaggtc ctcctgattt cctctgttct
    12841 tgtgtttgta ctgcttattt ccagggttgt tttgttgtca ctaaggcagt atattcttct
    12901 gtctcaggga aaatccttca acatacgcat agtggatggc ttctaccgat cgttgctggg
    12961 acttccgaaa ttgttctttg atacacgtaa gacaggcgac ctggttgcac gccttaatga
    13021 tacaatgcgc attcagagag tgatatccga tctcatcagt atctatttta ttgacattgt
    13081 tgttctgatt gtaacttctg tgctgatttt catatattcc ctgccagcag gtatcatttc
    13141 agtgatcgca attccattga tttatttact ggtttaccgt tggaataaaa gaatcatttc
    13201 atcccagaat gaattaatga ccggatatgc aatgaatgaa agcaatttca ttaattccct
    13261 tagaggcata gccgagataa aatgcatgaa ctggcagggt tattattctt tgaggaatga
    13321 atctgtcttc agcaatttcc aggaaaaatt cttctccctg ggcaaaatca gggtcagtct
    13381 gggaatgctg accaatctcg cgggaactct ttacctgatt gtattgctca tttattcatc
    13441 cttaaaggtt atggacctgc agatgacaca gggggaactg atggcaatcc tgtcgcttag
    13501 ctctaccctg ataccatcgg tgatgaatct ggcattgatt tccattccac tgagcgaagc
    13561 aaaggttgct atcgcaagga tgttcgaatt caccaggatc agtccggaag agactgattc
    13621 cggtaatata aaagagaata ttgcaattga aaaaatcagt cttaataata tgaagtttcg
    13681 cttcccgggc cagaagctgt ttctgacaga tatcaacctg gaaattgaaa aggggcaaat
    13741 aatcgcattg gtgggagaat caggttcggg gaaaagtact ttggtaaata ttcttttaag
    13801 attcctgatt ccggaatcag gtgatattat cataaatgat atcgttccgg tcaatcagat
    13861 aatgcctggc gtatggcgca gatcagtcgg agttgttccg caggatatac atatttttaa
    13921 cggaacaata ctggaaaatc tgattcctga accgtcagaa aaggaggttg aaattttatt
    13981 gcgccttatt tccgattacg gcctggaatc gtttattgag gccttgcctc tgggccttgt
    14041 caccctggtt ggggaagatg gagtaaagct ttccggtggt cagaaacagg ttattgcttt
    14101 tctcagggca ttgatcaatg aaccggatat tttactgatt gatgaaggta cctccggcat
    14161 ggataaggat acggaggacc tgatgctaaa aatgctgatc agcctgaaag cgaagctggg
    14221 aatcttgctg gtaactcaca gaatcagtct gattaaaaaa atctgtgaca ggatttacat
    14281 acttgaggac cgtaccataa aagaatcagg aactcattat gagcttatca gcagcgaaaa
    14341 tatatacaag cgttactggg aagaacttaa ttgaaaattc tatgaatatt gtattttttg
    14401 actgatattt actcatatgc tacattttca gaattgattt ccgggatcta gaaccctgtt
    14461 ttgagtgcaa cgttattttg aatgactgtt tttctttctg cctgggaagg cagaaataca
    14521 ggtaaacgat atttgtcata caaactaaaa gataatgaga atccggatat tctggatgcg
    14581 agaaccctga taacctgaga ttatgaaaag atatttctat accattaatt tcttcttgac
    14641 ggctatcttt ttttttgtag ccttagtgct ggatctgaaa ccttcaatac aaggttttag
    14701 ttttgttata ttttgttcct caatcggggt attgataggt gattatttga gaatcaggaa
    14761 aaatatcata ttgccagttg ctggaaaaaa actcatttcc tggattcttt ttataagcat
    14821 tgtatttatc ttattaatac tcttcctgat tgataatata ttaacaaaac agattctcat
    14881 tttttgtact ttactgacag gtgcattaat aactatattc attgtatttt ttaaaggaag
    14941 acctttgaaa aaagatgagc agggcaaaga ccggatccat tcaagctcac gttaggaaca
    15001 ggattatgcg gcccggattg attaacaact ttttctgtca gattatcctg ctagtgtgac
    15061 ctttttcaaa ttcttttcct actgctgtat tgatatttgt attgttcagt tataagcaag
    15121 gttaatgaaa ataacggttg taaatgagct aaccacccaa agtcgagcat gtgaaagaga
    15181 aaatcgacat taaataatag agtatccgat ttttagaatc gattattgtt taatcaagaa
    15241 gaagttttaa atcttgaaaa acttcctttt taaaaacaaa ttgatataaa aagctttttt
    15301 gtaagttttc ccggaatagt tgatattgca tcgttaattg tggtctcttg taaagaagat
    15361 aatcttgcta aagacttaag tatgaatttt agcaaacatc gataatgcac aaccaagcta
    15421 tggatttcga ctagttatgt aggtcagcgg cgttgccgcc gctttcccca ttaagaactg
    15481 tacgagcgac tttcaccgca tacagctccg ataattcaaa atttagttct cataaaacaa
    15541 aatcggctca tccgctcctt tgcaggaaac gatttccgat aaagtgccct ttcg
//
LOCUS       DAOSPX010000034        15584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3892_length_15584_cov_5.431708, whole genome shotgun sequence.
ACCESSION   DAOSPX010000034 DAOSPX010000000
VERSION     DAOSPX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3892_length_15584_cov_5.431708"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1293
                     /locus_tag="PLZ75_03025"
     CDS             19..1293
                     /locus_tag="PLZ75_03025"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOS71404.1"
                     /translation="MDKDEIIRLRSEAENHLVNELLPFWISRMTDRVNGGFITHFDRN
                     GMDTGEDEKSLIAQTRSVYTLSSAHRAGYGDGKYADLASHGVDFLINRMWDRKYGGFF
                     WMTDRRGNIKIDQKIIYGHSFAIYSLSEYTLATGDPRGIEYAEKVFDLLQKYCADTMY
                     GGYWEMFHRDWRLCGPGSQGGDRKTLDVHMHLMEAFTCLYECTGQDVHRRKLLEDIDL
                     LTIRLIHPVYKTGIPQFYKDWTIAPQIRFDIIWGWDRFSEDGQKGNATDNTCYGHNIE
                     FAWLLIHALNILGIDPSDYHELFRIIFDHTITNGIDYEYGGVFVEGPHSGGVYDREKE
                     FWQQAETLIGLLDAVIMFGDEKYWKAYKNVHRFVFDKVVNKGVGEWWPLLTRKGEPIW
                     THMSHSWKVNYHSVRAMVQSIKRLDCLIAGKA"
     gene            1543..2979
                     /locus_tag="PLZ75_03030"
     CDS             1543..2979
                     /locus_tag="PLZ75_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71405.1"
                     /translation="MKKNSGITRRRFIGTTTAAAAGFMIVPSNTVSGLGHKAPSDKLN
                     IAGVGIGGMGRGNIQRMADENIVALCDVEWGKNRRTNVAEVFQAYPKARKYYDWRQMY
                     DEMGKSIDAVMCATADHTHAIISAHAIALGKHVFCQKPLTHSVYESRLLTRLAAKHRV
                     ATQMGNQGSSAEGVRLICEWIWNGEIGEVRKIEAYTNRPIWPQGLTAPTEEQPVPETL
                     KWDLFIGPAKFRPYNEAYTPWVWRGWWDFGTGALGDMACHVMHPVFKSMMLQYPTKVI
                     GSSTPFTTDSPPNAEIMHLTFPERKKPADIKIDLPEVHVTWYDGGLKPGFPENWPADR
                     VQNSGVLFHGTKDILVCGEYGTDPILLSGRVPEVPKKLRRIEGTHEQDWIRACKESPE
                     SRVPAASDFSEAGPFNEMVVMGVLATRLQSLNKVLEWDGLNMTFKNVTDSDKITLGRN
                     ETVSAKAFADELIRHTYREGWSLPDMPA"
     gene            3167..3844
                     /locus_tag="PLZ75_03035"
     CDS             3167..3844
                     /locus_tag="PLZ75_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71406.1"
                     /translation="MIRITTLIVTGFFSLAGLSGYSQQTECAVKMESISGEYSGKCKK
                     GLAHGQGIASGTDRYEGEFRNGLPHGTGKYTWANGDYYRGGWKKGLKEGEGRLVKADS
                     SVVEGIWKQDSYVGKKLIAAYKINKSMSVTRYSFRKTNSTANDVKVKLIRGGIENSGV
                     DNLIIAHTSGSQYRSGSHTGIQNPLFPLDIKVTFTAWNMFRTAKSDVIFDFTINQPGS
                     WEVVINY"
     gene            complement(3951..4469)
                     /locus_tag="PLZ75_03040"
     CDS             complement(3951..4469)
                     /locus_tag="PLZ75_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOS71407.1"
                     /translation="MDYYELIQSRESIRNYDPDRPVPENTLKKILDAGRLAPSARNIQ
                     PWKFLVVSSREMLGKLRECYPRDWFRDAPHILVVAGMKDKAWTRSWDGYNSIETDLAI
                     AMTHILLAAANEGVGTCWVAAYNPEVLRQALELEESEVVYGITPLGFPKPEFVRKGAK
                     NRKSLDEIAEFL"
     gene            complement(4610..6280)
                     /locus_tag="PLZ75_03045"
     CDS             complement(4610..6280)
                     /locus_tag="PLZ75_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71408.1"
                     /translation="MKKSIYFAGIIILFISGISCSDDFLSKNEKNLYELNDTLKLDNR
                     QTNVQVPVQLPVLTDGSYTLFMQPKWLSFNVMKGKVTKGKFILDFDILNDYYKNVYEI
                     FYATVIIDIENLGLVGFTVSYENSGFPVLEFTPSVLRFDSFNYQTLTIRNTSEGLLKW
                     KITDIPEWLYLYPEAGSLQKDQSTMVMIHILPDPLKIYGQTSAELQLVSNSTNGNLIL
                     PVQIDESLVVQKDLVEIAGTVTDAEFSQESGIMAVITKSPDNLIIYNTSTKESRILSL
                     PHSPKCISISEEGDKAVIGYNTNLAGYWDLDNSELLRDYTIDCTPFDIVFGENGWCYI
                     SPDEGGFTGLRNLNLATGELSVTGSSSVLIYEKTTIAKMQGKPFIVGTRILTPSGILI
                     FDISGGRANDKITYYHTCADQFWIKKDGTRLYTYHRLVYYLPPYDGEYHPFDPPVFAH
                     IESSSPYITSFGESPGSGTIYTASTFYDNDPQYNAVIEQYYSGNLRKIRTFYVSPVFI
                     NENGTKELYKTSVRYLFADKYGSYVYAVKNLREYYHRDYWTIETVDVR"
     gene            complement(6277..7137)
                     /locus_tag="PLZ75_03050"
     CDS             complement(6277..7137)
                     /locus_tag="PLZ75_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71409.1"
                     /translation="MIRPLLAANKRAGRLLLIPVLFSFLVTAVYPQDKTISPDKDIKG
                     LRNKGTPGNLKDVTAFEEMFTGSTSNSRIRFDMNAGAGYLLAGTWKARDALVSQGADP
                     RGAESYYKNLKTGWSTGAGLTYMIKPQLGAGFRYRFFYTSGSLTGFFDPQDGIHLLYG
                     TYGEKICVNFYGPSLSYHISPGYRKLQVHFAYSAGMTTYRNEAEYINNYLLISGRNLG
                     IDASMGLEYFITDYLSAGAELSSFVTSLRKINLSDGTNSSSVKLDKKNYENLSRIELL
                     LGIRFYFWNR"
     gene            7326..7520
                     /locus_tag="PLZ75_03055"
     CDS             7326..7520
                     /locus_tag="PLZ75_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71410.1"
                     /translation="MKRKSRKSLYFFIPLSLVIKIILTVLLFNLPGGCREAREHFQEL
                     LEPGSEFAYFVDPYQNSENE"
     gene            7567..8013
                     /locus_tag="PLZ75_03060"
     CDS             7567..8013
                     /locus_tag="PLZ75_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS71411.1"
                     /translation="MKITEIKEYNEKVFRAVRTLLPQLDPAIILPSDDFLRGIIEGRD
                     TFLFVAEDENGDIRGILTLVRYDIPTGTKFWIEDVVVDESQRGKGYGRGLMIHAIRFA
                     ESHGARKIDLTSRPERIAANRLYQDLGFIRRETNFYRYLPESVRKS"
     gene            complement(8738..9499)
                     /locus_tag="PLZ75_03065"
     CDS             complement(8738..9499)
                     /locus_tag="PLZ75_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOS71412.1"
                     /translation="MEQTPSILLIAVIIFLFIILASAIKILREYERAVVFRLGRLVGV
                     KGPGLILLIPVVDKMVKVSLRTVVLDVPPQDVITKDNVSIKVNAVVYFRVMLPDKAII
                     EVENYLFATSQLSQTTLRSILGQSELDDLLSHREKINMELAKIIDHQTEPWGIKVSNV
                     EVKHIDLPQEMQRAMAKQAEAERERRAKVIHAEGEFQASTRLADAANIISANPTTLQL
                     RFLQTLTEVASEQSSTIIFPVPLDIISAFMQREKK"
     gene            complement(9522..10949)
                     /locus_tag="PLZ75_03070"
     CDS             complement(9522..10949)
                     /locus_tag="PLZ75_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOS71413.1"
                     /translation="MRFVIFHIILFSLLLQVRLPATAGGRSPETGRAMPHTTLWEIKP
                     AESAESLALTGPAAARKTVQEQGSRVRKVHLISVDASINPSSTEYIQSGIRRAEQENA
                     ECLIIKLNTPGGLLKSTRMIVTDILDSDVPVVVFVYPGGSQSASAGVFITLAAHIAAM
                     APGTNIGAAHPVSLQGQQDSVMIEKATNDAAAFIRTISEKRERNVQWAEDAVRKSLSI
                     TETEAMKMNVIDLIAISVPDLLEQIDGKEISLSSGTRILETANAEVVDIEMDFKQKLL
                     SLLSDPNIAYILMMLGIYGIMFELYSPGAIFPGVIGGISLIVAFYSLQTLPVNYAGLA
                     LIIFAIILFILEIKIVSHGLLSIGGIISLILGSVMLINTESTLEVIKISWYVILVIVI
                     LTAAFFLFAIGFAIKAQTRKPTTGVEGLVGETGTVITDLDPEGQVSVHGEFWFAESPD
                     GHLGKGTKVRIIRVSNMKLIVQKVI"
     gene            11431..11601
                     /locus_tag="PLZ75_03075"
     CDS             11431..11601
                     /locus_tag="PLZ75_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71414.1"
                     /translation="MKKVKPEEPLKARKLIRINDYTWIEKKANESDEATRERFLQKLA
                     KAENARDYHSEF"
     gene            complement(11633..12037)
                     /locus_tag="PLZ75_03080"
     CDS             complement(11633..12037)
                     /locus_tag="PLZ75_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOS71415.1"
                     /translation="MDTEEFLRRDHFASLLGIELFEAENGKARASLKIGEKHLNGLNM
                     AHGGAIFGLADLVFAAASNSYDRVAVGIHADIYFLRPVRKGEVLTGEASELSKSDKLA
                     TYAIKITNDQDELIASFRGMVYRKKESLERKS"
     gene            complement(12124..12840)
                     /locus_tag="PLZ75_03085"
     CDS             complement(12124..12840)
                     /locus_tag="PLZ75_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS71416.1"
                     /translation="MALKIDRLNKNYDEITLFRDFSISFTEGIITCILGPSGCGKTTL
                     LNIIGGITRPGSGNMSGFEDKRMSYIFQDPRLLQWKTVRGNIEFVMNRNMTDRERHRE
                     SDRLIRLVELEGFAGYYPSQLSGGMRQRVSIARAFACHSDIILMDEPLKGLDIALKQN
                     MIRSFTRIWKTDRRTVIFVSHDVEEAILLGEEIIVLSRPPVKIVAHENNNEPFESRRS
                     GSAATERLKQILFSSLGQNG"
     gene            complement(12843..13598)
                     /locus_tag="PLZ75_03090"
     CDS             complement(12843..13598)
                     /locus_tag="PLZ75_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS71417.1"
                     /translation="MKISITKKQYLGFASVVAMLILWQLLAMYFDSDVIVPSPGKTLI
                     TAIGLFNDPDFISVVGTTIIRGLAGFVISAFLGMLLGIIAGLSSGFNALLQPFLVTVR
                     SVPVIALILLALIWFTPGSVPVFIALLTMFPFICTNVIDGIRSVDPDIIVMAKFYRIS
                     RSRIISEVYIPAIMPFIISGASSAMGIGWRAIIIGEVLSQPRYGIGTMMQAEQTFLNV
                     DAVIAWTIIAVLISYCFEKVIRWSEHKIVTWRD"
     gene            complement(13579..14541)
                     /locus_tag="PLZ75_03095"
     CDS             complement(13579..14541)
                     /locus_tag="PLZ75_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS71418.1"
                     /translation="MRSNLFILIFILLVCSACGGHGKRSHERKFLTIASLKGPSSMGI
                     IRFIDSVYNDSAKNIKISIQDEPIQVRKMILDGSADFALLPSNMAAILYNMGFDYRLA
                     AIPVWGTLYLFGGDTSVKSWEDLRNKRVYAMARGMTPDVLFRFLLEKNGLDPDRDIIL
                     DYSFPTHLNLSNAVAAGKADLAVLSEPQVSLVMKRNKNVHRIFDLNSEWNKVQGFPIA
                     QTAFIVREDLLRNDPGLAERIISACERSVRWVNSYPDSAAVLIVKYNILPDAEVAVSA
                     IPGSNMNFVRARGIREEIEKYFAVFYKMNPDIIGRKMPDENFYY"
     gene            14844..15098
                     /locus_tag="PLZ75_03100"
     CDS             14844..15098
                     /locus_tag="PLZ75_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71419.1"
                     /translation="MEKRIGTVLIRIDSRESVHLLNEIISDHSDIIIGRQGLPREYSQ
                     SIISLVLEGTTDQIGSLTGRIGRLSGIRAKSVLLKNKENE"
     gene            15139..>15584
                     /locus_tag="PLZ75_03105"
     CDS             15139..>15584
                     /locus_tag="PLZ75_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOS71420.1"
                     /translation="MSSRGQERNQQKLKSDTLTLTEKTVNLGEVVVSSLRLDRQVKKL
                     PVPLAVVRSFDYQKQSSLTLSNVLEKKSGISMGGDGVWATNINIRGLSENRLVTLIDG
                     NRVETANDLTASMSMIDMYDVERVEVIKGAQSSLYGSGAMGGIVNI"
BASE COUNT         4275 a         3399 c         3606 g         4304 t
ORIGIN      
        1 acaaataaat aattttcaat ggataaggat gagataatca gattaagatc agaggctgag
       61 aaccatctgg tcaatgagtt attgcctttc tggatttccc gtatgaccga cagggtcaac
      121 ggtggtttca tcactcattt tgacaggaac gggatggata cgggcgagga tgaaaagtct
      181 ctaattgccc agacccgttc ggtttacact ctttcttccg ctcaccgggc cggatacgga
      241 gacgggaaat acgcggatct tgcaagtcat ggtgtggatt tcctgatcaa ccggatgtgg
      301 gaccggaaat acgggggatt tttctggatg acggaccggc ggggaaatat taagatcgat
      361 cagaaaataa tttatggtca cagttttgca atttattctt taagtgaata taccctggct
      421 acaggtgatc cccgcggaat tgaatatgca gaaaaagtct ttgacctcct tcaaaaatat
      481 tgtgcagata ccatgtacgg gggttactgg gaaatgttcc accgcgactg gaggctttgc
      541 ggaccgggca gtcagggtgg tgacagaaag accctcgatg tgcacatgca tcttatggaa
      601 gcctttacct gcctttacga gtgcactggt caggatgtgc accgccggaa actgcttgaa
      661 gatattgacc tgttaacaat acggcttatt cacccggtat ataaaaccgg aataccacag
      721 ttctataaag actggacaat agctccccag atcaggtttg atataatctg gggatgggac
      781 aggttttcgg aagacgggca gaagggaaat gccaccgaca atacatgcta tggtcataac
      841 atagaatttg catggcttct gatccatgcg ctgaacatcc ttggcattga tccctcggac
      901 tatcatgaac ttttcagaat catattcgat catactataa ccaacggcat tgattacgaa
      961 tatggcggcg tttttgttga aggacctcac tccggcgggg tttatgacag ggagaaggag
     1021 ttctggcagc aggccgaaac tctgatcggc ctccttgatg cagtaatcat gtttggtgat
     1081 gaaaaatact ggaaagccta caaaaatgtg caccgttttg tttttgacaa ggttgtaaac
     1141 aagggtgtgg gagaatggtg gcctttgctg acaagaaaag gggaaccgat atggacacac
     1201 atgtcgcatt catggaaggt caattatcat tcagtcaggg ccatggtaca gagcatcaaa
     1261 agactggact gtctgattgc aggaaaagct taagctgctg acaggcatac aggcggtgtt
     1321 attgatgtat tatgctgttg tacatgcatc cgggaaaaaa gatttcttcc ggatcactgg
     1381 ctgactgatt ttaaaatttg tacaggattc tgtttttaac tgctaataat atggggagga
     1441 actgtcagga tcacaggatg aacgaacaac gaaagacatt ttttcaaaag ataaacaata
     1501 tcaggatctt atattgaatt attaatcaaa acttcgtttt atatgaaaaa gaactcaggg
     1561 attacgcgaa ggagatttat tggaacaaca acagctgccg cggcaggatt tatgattgtt
     1621 ccttccaata ctgtttccgg gctggggcat aaggctccga gtgataaact gaatattgcc
     1681 ggtgtgggga tagggggtat gggaaggggc aatattcaga gaatggctga tgaaaacata
     1741 gtcgctctat gtgatgtgga atggggcaag aacaggagaa ccaatgttgc agaggttttt
     1801 caggcttacc cgaaagccag gaagtactat gactggcgtc agatgtatga tgaaatgggc
     1861 aagtcgatag atgctgtcat gtgtgcgact gccgaccata cgcatgctat catttcggct
     1921 catgcgattg cactgggcaa acacgtgttt tgtcagaaac ccctgacaca cagtgtttat
     1981 gaatcacgtt tactgaccag gctggctgca aagcacaggg tagcaaccca gatgggaaac
     2041 cagggttcgt ctgccgaagg agtcagactg atctgtgaat ggatttggaa tggtgaaatt
     2101 ggtgaggtaa ggaaaataga agcctatacg aaccgtccca tctggcctca gggcctgaca
     2161 gccccgacag aggaacagcc tgttcccgaa acactcaaat gggacctgtt tatcggtcct
     2221 gcgaaattcc ggccgtataa tgaagcctat acgccctggg tatggcgcgg ctggtgggat
     2281 tttggcaccg gggcccttgg agatatggcc tgccatgtga tgcatccggt tttcaaatca
     2341 atgatgcttc aatatccgac aaaagttatc ggcagttcca cgccattcac aaccgacagt
     2401 cctcccaatg ccgaaataat gcatcttact ttccctgaac ggaaaaagcc ggctgatatt
     2461 aaaatcgatc tgccggaagt gcacgttaca tggtatgacg gcggattgaa gccgggattc
     2521 ccggagaact ggccggcaga cagggtacag aattccggtg tgcttttcca cgggacaaag
     2581 gatattctgg tctgcggcga atatggtaca gatccgatcc ttctttcagg aagggttccg
     2641 gaggtaccga agaaacttcg ccgtattgag ggaacccatg aacaggactg gatccgtgcc
     2701 tgtaaggaaa gtcctgaaag ccgtgtgccc gcagcatccg atttcagcga ggccggtcct
     2761 ttcaacgaaa tggtggttat gggtgtcctt gcaacacgtc tgcagtctct taataaagtg
     2821 cttgaatggg acggattgaa catgacattt aaaaatgtca ccgatagtga taaaataact
     2881 ctgggcagga atgaaaccgt ttctgccaaa gcattcgcgg acgaactgat caggcatacc
     2941 tacagggagg gatggtctct gcctgacatg cctgcatgat tcttatgata aatgcgctgc
     3001 tgaaaatgat cagcagcgca cggataaacc ggaatcatca gcaataattc ctgctgcaga
     3061 caggtttcag gtaaggagca ccggctgatt gtttaaaatt aattgcctgt cagatgaata
     3121 tggaaaaata aaatacgata tttgttacaa tcagagaaca gggactatga tacggataac
     3181 aacactgata gtaaccgggt ttttcagcct tgccggttta agcggatatt cacagcagac
     3241 ggaatgtgcg gtaaaaatgg aatctatctc gggtgaatat tccgggaagt gcaaaaaggg
     3301 actggcgcat gggcagggta ttgcttccgg aaccgaccgt tatgaaggag aattcaggaa
     3361 cggattgccg catggaacag gaaaatatac atgggccaac ggtgattatt acaggggagg
     3421 gtggaaaaag ggactgaagg aaggagaagg ccggctcgtg aaggcagatt catcggttgt
     3481 ggaaggtatc tggaaacagg acagctatgt cgggaagaaa ctaattgccg cttacaagat
     3541 aaacaaaagc atgtccgtta ctaggtattc tttcagaaag accaacagta ccgcaaatga
     3601 tgtaaaggtg aagctgataa ggggagggat agagaattca ggtgttgaca atctgataat
     3661 tgcacatacc agcggttccc agtacagatc gggatcccat accggtatcc agaatccgct
     3721 gttccccctg gatataaagg tcaccttcac tgcctggaac atgttccgta ccgccaagtc
     3781 cgatgttata tttgatttca caataaacca gcccggcagc tgggaggtag taatcaatta
     3841 ttagacttca tgctgcacta tatattttat ctcccggatc cttgccgttt aaagagaaat
     3901 gtgccgtttt gcctgaacac ccgtaacgtg acaggattcg tgacttgcag tcagaggaat
     3961 tcagctatct catccaggga cttcctgttt tttgctccct ttctgacaaa ttccggtttc
     4021 gggaatccga gcggagtaat tccgtaaaca acttcagatt cttcaagttc aagggcctgc
     4081 cttaaaactt ccgggttata tgctgcaacc cagcaggttc ccacaccttc atttgcagct
     4141 gccagcagaa tatgggtcat ggcaatggcc agatccgttt ctatggaatt atagccgtcc
     4201 caggaacgtg tccaggcctt gtctttcatt ccggccacga caaggatatg gggagcatcc
     4261 cggaaccagt ccctaggata acattccctc agctttccga gcatctcccg ggaagagaca
     4321 accaggaatt tccagggctg gatattgcgt gctgatggcg ccagcctgcc tgcatcgaga
     4381 atttttttaa gtgtattttc gggaacaggt ctgtccggat cgtagttacg gatgctttca
     4441 cgcgactgaa tgagttcgta ataatccatt tttctgtttt tgggttgagc ggaagccagt
     4501 attccggaat ccggtcatat agtcaggaac gggttttccg gatgatctcc aaagttaaga
     4561 atcaatattg aattgtccgg cagaggtaac gcatctgttt aaagatttgc taacggacat
     4621 caacggtttc gattgtccag taatccctgt ggtaatattc cctcagattt tttacggcat
     4681 agacgtagga cccgtacttg tctgcaaaca ggtatctcac tgatgtttta tataattcct
     4741 tcgtgccgtt ttcattaata aataccgggg aaacatagaa tgtccttatt ttccggagat
     4801 tcccggaata atattgttct attacagcat tgtattgggg atcattgtcg taaaatgttg
     4861 aagccgtgta aattgtgccc gatccgggcg attcaccgaa tgatgtgata tagggtgatg
     4921 aggactctat atgtgcgaat acgggcggat caaacggatg atattccccg tcataggggg
     4981 gcaggtaata aaccagcctg tgataggtat agagcctggt gccgtctttt ttaatccaga
     5041 actgatctgc acatgtatgg taatatgtta ttttatcgtt tgctctgcct ccggaaatat
     5101 cgaaaataag gattcccgaa ggagtcagta tcctggtccc gacaataaac ggcttcccct
     5161 gcattttggc gatagtggtt ttttcataga tcaatactga agaagatcct gtaaccgaca
     5221 gttcccccgt tgcaaggttg agattcctga gacccgtaaa tcccccttca tccggagaaa
     5281 tatagcacca gccattttcg ccaaacacga tatcaaaggg agtgcaatca atagtgtagt
     5341 ccctgagcag ttctgaattg tcaagatccc aatagccggc caggttcgtg ttatagccta
     5401 ttacagcctt atccccttcc tcagatatgc ttatacattt cgggctgtga gggagggaaa
     5461 ggattcgtga ctcttttgtg ctggtattgt agatgatgag attgtcgggc gattttgtta
     5521 tgacagccat tatacctgat tcctggctga attccgcatc agtcactgtc ccggctattt
     5581 ccaccagatc tttctgaacc accagggatt catcaatctg aaccggcagg atcagattac
     5641 cgttggttga attgctgacc aactgtaatt cagctgatgt ctgtccgtaa atttttaaag
     5701 gatccggaag gatatgaatc attaccatgg ttgactggtc tttctgcaga gagccggctt
     5761 ccggataaag gtacagccat tcgggtatgt ctgttatttt ccacttcaga agaccctcag
     5821 aagtattcct gatggtaaga gtctgataat tgaatgaatc gaatctgagt acggaaggtg
     5881 tgaactcaag gacagggaat ccggaatttt cgtaggaaac cgtgaaacca accagtccca
     5941 gattttcaat gtcaataatg acagtcgcgt agaaaatctc atagacgttt ttataatagt
     6001 cattgaggat atcgaaatcg agtatgaatt ttccctttgt caccttgcct ttcataacat
     6061 tgaaagacaa ccattttggc tgcataaaca gggtgtagct cccatcggtt aaaaccggaa
     6121 gctgtaccgg cacctgtaca tttgtctgcc tgttatccag tttcagggta tcgttgagtt
     6181 cataaagatt cttttcgttt tttgaaagga aatcatcact gcatgagatt cctgaaatga
     6241 aaagaataat tattccggca aaatatattg attttttcat ctgttccaga aataaaacct
     6301 gatacccagt aataattcaa ttcgtgagag attctcataa ttctttttat caagtttaac
     6361 acttgagctg ttggtgccgt ctgataaatt tatttttctg agtgatgtaa caaaggatga
     6421 aagctcagcg ccggctgaca gatagtctgt gatgaaatat tccaggccca tggaagcgtc
     6481 aattcccaaa ttccggccac ttataagcag atagttattt atatattcag cctcattcct
     6541 gtaggtggtc attcctgctg aataggcaaa atgtacctgc agcttacggt atccgggact
     6601 gatatgatag ctcagggatg gaccgtaaaa attgacgcaa attttctccc cgtaagtacc
     6661 atagagcaga tggatcccgt cctgcgggtc aaagaaaccg gtcagacttc ctgaagtata
     6721 aaagaatctg tacctgaatc ctgctcccag ttgcggtttg atcatgtaag taagcccggc
     6781 tcctgtactc catccggttt tcaggttttt ataatatgat tccgccccgc gtggatctgc
     6841 accctgactt acaagggcgt ccctggcttt ccatgttccg gccagtaaat atccggcacc
     6901 ggcattcata tcaaaccgta tcctgctgtt tgaagtgctg cccgtgaaca tttcctcaaa
     6961 ggcagttaca tcttttaagt ttccgggggt ccccttgttc cttaaacctt ttatgtcctt
     7021 atccggggat atcgtcttat cctgcggata aaccgcagtt accaggaatg aaaacagtac
     7081 cgggatcagc aggagtctgc cggctcgttt atttgctgct agaagcggac ggatcattgt
     7141 ttctcagggt tgtgctaatt gtgggaatgg ataacggaaa ctgttgaatt ctactttttt
     7201 tgccagcatt taagatgcta aagtaaatat tctttcatca gactgtaccg gctgaatgaa
     7261 attttttttc agttcctgaa acaaaagctg ctcaccggcg tttatcgcag taatattact
     7321 gtaccatgaa aagaaaaagc aggaagtcac tttatttctt catacctctg agccttgtaa
     7381 tcaagattat actgactgta ttgctgttta atcttccggg cggatgcagg gaggcaagag
     7441 aacattttca ggaactgctt gaacctggtt cggaatttgc ttattttgtg gatccatacc
     7501 agaattcaga aaatgaataa ctcctccttg cagtgtgtaa gctgcggaaa caaataaata
     7561 gtcaatatga aaataactga aataaaagag tataatgaaa aagtattcag ggcagtccgg
     7621 acactgctgc cgcaacttga cccggccata attcttccgt cagacgattt tctaaggggc
     7681 atcatagaag gcagggatac ttttcttttt gttgcggagg atgaaaatgg tgatatcaga
     7741 ggcattctga cccttgtcag gtatgatatt cccaccggaa ccaaattctg gattgaagat
     7801 gttgttgtgg atgaatcgca aagaggaaaa ggttatggca ggggcctgat gattcatgca
     7861 atcagatttg ccgaatcaca cggagcaagg aaaattgatc tgacttccag accggaaaga
     7921 atagctgcaa accgcctgta tcaggatctt ggatttataa ggcgggaaac aaatttttac
     7981 agatatctgc cggaatcagt cagaaaatcc tgaatctgct gactgaatct gccgaaaaac
     8041 ccttttttca tcataataaa gcgtcagctg gtcaaaaccg tcccgccgga cgtcaatatt
     8101 caattcattt tgacgaatgc aggaatatta tatgccagtt aaggctgtgg cttaacggta
     8161 ctgcagaatt tattgaccag gctttgccgg gaatgctcca aaccccgtca gatggacatt
     8221 accagtttgt gagagagctt tcgggtactt atctttggaa tggtaataat ttgaagatca
     8281 tgaaggctgt ctatcacata aaatcaacag taacaggatc tgttaagctt aaacgcagga
     8341 agattgcaaa agcactccgg tggtggttgc gcgagaatgg ttttgagttt agttacagtt
     8401 attcctgcag ttaataacat aatccgtaat gcctgaaacc ggaatgaaac gtcatttgaa
     8461 agatattttt cccttactga ttctctcctt attgtggctg ggtctttctt ctttttcggt
     8521 tccggatgtg aagcccggcc gggtaaaaaa agagcagcgc agggaatgca ggaaacagaa
     8581 cggcagctgt attgtcagca ggcaaaaaca ctatccaacg ccattcagta ataaaaaaat
     8641 aaaaaccaaa tccaatcgca ggatcaaatc agaccggaga tgagtaaaac aggcagttcg
     8701 cctgtcttac tcagaactga ccggtagtat cagtatctca tttcttttcc ctttgcatga
     8761 atgccgaaat gatatcgagc ggtaccggga atattatagt ggagctctgt tcggatgcca
     8821 cttccgtaag ggtttgcagg aaacgaagct gcagggtagt gggatttgca ctgattatat
     8881 ttgctgcatc ggccagccgg gtgcttgcct ggaattcacc ttcggcatgt ataacttttg
     8941 cacggcgttc tctttcagct tcagcctgtt ttgccattgc acgctgcatc tcctggggaa
     9001 gatctatatg ttttacttca acatttgata cttttatccc ccagggttcg gtctgatggt
     9061 caataatctt ggccagttcc atatttattt tttcacggtg tgaaaggagg tcatccagtt
     9121 ccgactgtcc cagtatgctt ctcagggttg tctgcgaaag ctgggaggtt gcaaacagat
     9181 agttctccac ttcaataatt gccttatccg gcagcatcac ccggaaataa accacggcgt
     9241 ttacctttat cgagacgttg tcctttgtaa tgacatcctg cggcgggaca tccagcacta
     9301 ccgttctgag gcttaccttt accattttgt cgaccaccgg gatcagcagg atcaggcccg
     9361 gtcccttcac accgaccagt cttccgagac ggaaaaccac agcacgttca tattcgcgta
     9421 aaatctttat tgcacttgcc agaatgataa acaggaatat tatcactgct atcaaaagaa
     9481 tactgggtgt ttgttccata atgattccct ttctgtttga tttaaattac tttttgaact
     9541 attaatttca tgtttgaaac cctgataatc ctgacttttg tgccttttcc caaatgtccg
     9601 tcaggacttt cggcaaacca gaactccccg tgaacactta cctgtccctc gggatcaagg
     9661 tcagtgataa cagtgcccgt ctccccgaca agtccttcca caccggtggt gggcttacga
     9721 gtctgtgcct tgatggcaaa gcctatggca aagaggaaga atgctgccgt aagtatgaca
     9781 attaccagga ttacatacca ggatattttg attacttcaa gtgttgattc cgtgtttatt
     9841 agcatgactg atccaagaat tagtgatatt attccgccta tggataaaag cccgtgactg
     9901 actatcttta tttctagaat gaagaggatg attgcaaaaa ttatcagtgc caggcctgca
     9961 taattaaccg gcaatgtctg cagggaataa aatgcaacga tcaggcttat tcctcctatt
    10021 actcccggga atatggcacc cggactgtaa agttcaaaca tgataccgta tattcccagc
    10081 atcatcagga tatatgcaat gttcgggtca ctcagcaggc ttagtagctt ttgcttgaaa
    10141 tccatttcaa tgtcaacaac ttcggcattt gcggtttcca gaatcctggt accggatgag
    10201 agacttattt ccttgccgtc tatctgttcg agcagatccg gaacggatat ggctatcagg
    10261 tcaataacat tcatcttcat tgcctcggtt tcggttattg acaggctctt tctgacagca
    10321 tcctctgccc actggacatt cctctccctt ttctcgctga tggttcttat gaatgctgcc
    10381 gcatcgttgg tggccttttc aatcattacc gaatcctgct gaccctgaag ggaaacgggg
    10441 tgagcggcac caatgtttgt tcccggtgcc attgccgcaa tatgtgctgc aagggtgatg
    10501 aaaactcctg ccgaggcaga ctgtgatccg cccggataaa caaacaccac aacgggaaca
    10561 tcggaatcca gaatgtcagt aacaatcatc ctggttgatt taagcaatcc gcccggtgta
    10621 ttcagtttaa tgataagaca ttcagcattt tcctgttcgg cacgtctgat ccccgactga
    10681 atgtattcag tacttgaagg atttattgag gcatcaaccg aaatcaaatg taccttcctg
    10741 actctgctgc cctgttcctg aactgtcttc cgtgccgcag ccggacctgt taacgctaag
    10801 ctttcagctg attctgcagg ctttatttcc cataaggttg tatggggcat tgcccggccg
    10861 gtttcaggcg acctgccacc ggcagttgcc ggcagcctta cctgtaacag caggctgaaa
    10921 agaattatat gaaatattac aaatcgcaac ttattaaatt ataaaactat aacagctgtt
    10981 attaattatt gaaaggctgt aaagataatc gttatcctgg tcttactctt atgttactaa
    11041 aatcaaattt aatttaattg atctatattt cataattttc attatacaat aattgatgct
    11101 gataccgtga tggaatctct ctgctattga ttattaagta aatgtgctca ggcccctgcc
    11161 tggtacctgc ggccgtttct ttccgggccg ggtttatata ggcatgtact gttttagtac
    11221 ttcctgtagt acttcctgat ttaccggtcc ggatgttaaa aaggcccgga aaaatgttaa
    11281 taagtaaatc actgtcaatt cattattcta tatatattta ttctcagcaa tacgattcct
    11341 gaaatagggt ttgaatatac tcagaagtct gaaacacaga tgatgtgaaa ataatatgtc
    11401 aatgtcttat acttcaaaaa ttactgtgct atgaagaagg ttaaacctga agaaccgctg
    11461 aaggcaagga aacttatccg cattaacgat tacacctgga ttgaaaaaaa agcaaatgaa
    11521 tctgatgagg caacgcgtga aagatttctt cagaaacttg caaaggcgga aaatgcccgg
    11581 gattatcact cagaatttta gacaggactg catatttctg gcggagctca gttcatgatt
    11641 tccgttcaag gctttccttt ttgcggtaaa ccattccccg gaagctggca ataagctcat
    11701 cctgatcgtt tgtgattttt attgcatagg tggcgagctt gtcactcttg gataattctg
    11761 acgcttcgcc ggttaaaacc tcacctttgc gtaccggtct caggaaatat atatctgcat
    11821 gaataccgac ggcaaccctg tcataggagt ttgacgctgc cgcgaacacc agatctgcaa
    11881 gaccgaatat tgctcctccg tgagccatat taaggccgtt caggtgtttt tcacctattt
    11941 tcagacttgc cctggctttc ccgttttcag cctcgaataa ttcaattccg aggagcgagg
    12001 caaaatgatc ccgtctgaga aattcttctg tgtccatatt tttaggttta aaattttatc
    12061 atgaggcaat gaaagcaaga tcagtggaaa tctccgcact gaattgcggc ttccggtctc
    12121 cgctcagccg ttctgcccga gagaactgaa caatatctgc ttcagccgtt ccgttgcggc
    12181 tgaccctgaa cgccggctct caaagggttc attattgttt tcatgtgcca ctattttcac
    12241 agggggacgg ctcagtacga ttatctcttc accaagcaga atagcttcct caacatcgtg
    12301 actgacaaaa attactgttc tcctgtcggt tttccatata cgcgtgaacg atcttatcat
    12361 gttttgtttt aatgcaatat ccagtccctt caggggttca tccatcagaa tgatatcgga
    12421 atggcaggca aaggcccttg caattgaaac ccgctgacgc attcccccgc tgagctgtga
    12481 gggataatat ccggcaaaac cttccagttc aaccagccgg atgagacggt cactttccct
    12541 gtgcctttcc ctgtctgtca tattcctgtt catcacaaac tctatgtttc cccttacggt
    12601 tttccattgc agaaggcggg gatcctgaaa gatatatgac atccgcttgt cttcaaagcc
    12661 gctcatattc ccgctgccgg gacgggtgat accgccgatg atattcagca gtgtggtttt
    12721 tccgcatccg gaaggaccca ggatacaggt tattatgcct tcagtgaagc ttatgctgaa
    12781 atccctgaac agagtgatct catcataatt cttgttcagt ctgtcgatct tcagagccat
    12841 aatcagtctc tccaggttac aattttatgt tcactccagc ggatgacttt ctcgaagcag
    12901 taactgatga gaactgctat gatcgtccat gcaataactg catcaacgtt cagaaaagtc
    12961 tgttctgcct gcatcattgt gcctattccg tatctgggct gactcagaac ctcaccgatt
    13021 attattgccc tccagcctat acccatggca cttgatgccc cgcttattat aaatggcata
    13081 atggccggga tgtaaacttc ggaaattatt ctactgcggc ttattctgta aaactttgcc
    13141 attacaataa tgtcggggtc cacgctgcgt attccgtcaa tcacattggt gcagatgaag
    13201 ggaaacattg taagaagcgc aatgaaaaca ggaacggatc ctggcgtgaa ccagataagt
    13261 gccagcagga tgagggcgat gacaggaacc gagcggaccg tcaccaggaa gggctgcagc
    13321 agggcattga atccggaact aaggcctgca ataataccga gcagcatgcc aaggaaagca
    13381 gaaatgacaa atcctgcaag gccacgtatt attgtggtgc caaccacgga gatgaaatcc
    13441 ggatcgttga acagtccgat tgcagttatt aatgtcttgc cgggtgacgg aacaattacg
    13501 tcggaatcaa aatacatggc aagtaactgc cataggatca gcattgccac aaccgatgca
    13561 aaaccaagat attgcttttt agtaatagaa attttcatca ggcatcttcc ttccgataat
    13621 atcaggattc attttataaa aaacagcaaa gtatttttcg atttcctcac ggataccacg
    13681 ggcccttaca aagttcatgt ttgaacccgg tatggcactt accgctactt cagcatcagg
    13741 gagtatgttg tattttacga ttaataccgc agcactgtcg ggataactgt ttacccaccg
    13801 gaccgatctt tcgcatgcgg aaataatcct ttccgccagg ccggggtcat tccggagaag
    13861 atcttccctg acaatgaaag ccgtctgggc aatcggaaac ccctgaacct tgttccattc
    13921 actgttcaga tcaaatatcc tgtgcacatt tttattcctt ttcattacca ggcttacctg
    13981 gggttcggaa aggacggcaa gatctgcctt gccggcagca acggcattgg acaggttgag
    14041 gtgggttgga aaactgtagt caagtattat atccctgtca ggatcaagac cgtttttttc
    14101 aagaagaaag cgaaagagca catccggagt cattcccctg gccattgcat ataccctctt
    14161 gttcctcaga tcttcccagc ttttgactga tgtatccccg ccgaaaagat agagggtgcc
    14221 ccacacggga atggccgcaa gcctgtagtc aaatcccatg ttatacagta ttgcagccat
    14281 gttggagggg agaagggcaa aatccgcgct gccgtccaga atcatttttc tcacctgtat
    14341 gggttcgtcc tgtatgctga tcttaatgtt tttggcacta tcattgtaaa cgctgtctat
    14401 aaagcgtatt attcccatgg atgaaggccc tttcaggctg gcaatggtaa ggaacttacg
    14461 ttcatggctt cttttcccat gaccgccgca tgctgaacag acaagcagaa tgaagatgag
    14521 aataaacaaa ttgctgcgca tctgaaaaac tttacacaaa tctatttaat ttataacaca
    14581 gttatataat tttatatttc attctgcata acggaaaggt gtttgttttc cgtgaagaac
    14641 aactgccctg accagaatgc ctgatccggg atgcctgcat caacaggcct gcctgccgga
    14701 aacatgacca tagtcattgt tggaatcatt ataaacatct acttttgcag ttaagaaaaa
    14761 cctgtaaata ttactgaggt ttctgatttt attcagggcc aaggttctgc aggctgatat
    14821 taatctaatc caaaggtatt ctgatggaaa agagaatagg aacagtactc atccgcattg
    14881 atagtcgtga aagcgttcat cttctgaacg aaatcatctc agatcattcc gatatcatca
    14941 taggccgcca ggggctgccg cgtgaataca gtcaaagcat catatctctt gttctcgagg
    15001 gtaccacgga tcagatcggt tccctgaccg gccgtatcgg ccgtctgagc gggatccggg
    15061 caaaatctgt acttttaaag aacaaggaaa atgaataagg tattttacat ttcggcagtc
    15121 tgtttaatta ttttttccat gagctcccgc ggacaggagc ggaatcagca gaaactgaaa
    15181 agcgacactc ttacccttac agaaaaaact gtaaatcttg gtgaagtggt tgtctcgagt
    15241 ctgaggctcg accggcaggt aaaaaaactc ccggtaccgc tggctgttgt caggtctttt
    15301 gattaccaga aacaatcctc tctcaccctg tccaacgtgc ttgagaaaaa atccggaatt
    15361 tccatgggcg gggacggagt ctgggcaaca aatattaata tccgcggact gagtgagaac
    15421 aggctggtaa ccctgataga cggaaacagg gttgagaccg ctaatgacct gacggcatcc
    15481 atgagcatga tagacatgta tgatgttgaa agggtggaag tgattaaggg tgcacaatcg
    15541 tcattgtacg gttcgggtgc aatgggcgga attgtaaata ttat
//
LOCUS       DAOSPX010000035        15564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_3896_length_15564_cov_4.958218, whole genome shotgun sequence.
ACCESSION   DAOSPX010000035 DAOSPX010000000
VERSION     DAOSPX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15564
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3896_length_15564_cov_4.958218"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLZ75_03110"
     CDS             complement(<1..935)
                     /locus_tag="PLZ75_03110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOS71421.1"
                     /translation="MIRNIVLGTFMFVSFSLQSDTAAKIPVPGRYSFEADSFRNYLDR
                     RVPELMRHYKIPGVSLSVLKKGRILWSDSYGYADIQAGRKMTNDSYYRVESLSKSLTA
                     WGVMKLIEQGRLDPDQPVMTYFKNRVIPDSEYSEESVTVRMLLSHTAGMPLGSIGLRY
                     APGEKKPALRQQLLADAFLKQEPGRSFSYSNTGFNILEALIEEITERDFSEYMKNELL
                     LPLGMEHSSFDWNESFNDQIPKGYDLKGNPVPVYVYAEKASGGLFASVADIAAFLAAG
                     MTNDSVADRKVINTQSINELYMPRADIHGFYGLAF"
     gene            complement(988..3354)
                     /locus_tag="PLZ75_03115"
     CDS             complement(988..3354)
                     /locus_tag="PLZ75_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS71422.1"
                     /translation="MKRIIKSLLIALLLVLNSLTSPSQELVQTVRGRVTDAYTEVPIP
                     GVVIIIKDSDPLKGVVTDAEGFFRFGNLPVGRITLSVSSIGYKPEVLTNLLLTSGREL
                     IVEIRLTEQVYSLEEIVIRPNTRKDQPSNEIAVISARSFTIDETERYAGSLGDPSRMA
                     TNFAGVSSVSDQRNDIIIRGNSPLGLLWRLEGVEIPNPNHFGSLGSTGGPVSMLNNNL
                     LANSDFYTSAFPAEYGNALSGVFDLRMRNGNNQKREFLGQIGFNGFELGAEGPLHEKS
                     RASYLLSFRYSTLELLHAVGMSFGTGSAIPKYKDLSLKLNFPLNKGRLSLFALGGDNR
                     IAMLDSEGDDAQYGFSGTDLFYRNRMGVGGLSYVRYLTDNARLTGTIAVSGIEGIAEI
                     FDLDGRLQQKVIDENMHEIKYTFSTKYHQRFNSRNYLNTGLVIDIFGLKYTGAQFRKT
                     TGDYFYYMNSEGNTGYARLFSEWQHRFSDKLSLGTGLNTSYFFLNDSKSIEPRFGLKW
                     NNAPGQSFNLGAGLHSQTQMKAVYFSQNMVSAFPGHYEKTNEHLDLSRSIHLVAGYDR
                     LFGEGHRLKAELYYQQLYNIPVSKHQPEYSLINQGGGFSFVVLDSMENTGTGRNYGME
                     ITLEKFLQQGFYYLLTVSLFDARYRGYDGVQRNSAFNNRFVFNALSGYEWKLGRKSLL
                     SADIKIVYAGGSPYLEIDAERTRAENTLRYRWDRAYEMHFPNYFRMNGRITFRLNMNR
                     LNQEWALDLQNITNHKNIFTQNWNSSTKEISTSFQMGFMPMVTYRICF"
     gene            complement(3492..4250)
                     /locus_tag="PLZ75_03120"
     CDS             complement(3492..4250)
                     /locus_tag="PLZ75_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOS71423.1"
                     /translation="MKVVIIEDESFASLRLKKLIHDYDSSIEVIAELESVEESVKWFR
                     SNKEPDLIFLDIHLEDDLSFSIFEKVNISCPIIFTTAFDEYAIKAFKLNSIDYLLKPI
                     IQEELAAALKKYSGMTGQTGAGTDINALYRLMAERTPRYRERFSASFGTKIKTIAVDQ
                     VAYFVSEGGGVFAYLKEKGSYPVDISLDRLMAELDPKKFFRINRKILVGINGIGEIHV
                     YPKSRLKLDLNPKFHEDVFVSLDKVTSFKKWLDQ"
     gene            complement(4410..5759)
                     /locus_tag="PLZ75_03125"
     CDS             complement(4410..5759)
                     /locus_tag="PLZ75_03125"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOS71424.1"
                     /translation="MKNWHMDLDRELIREEQLKGARISVYFRWTFIFLVAVLLIVQLS
                     LGYRKESVHAFILIMVYFLSNIVLWYAVVRKYNPGYLGFASALLDVGVICAHYYILSA
                     EFDPLASSSAATIFLIPLLFLLYTFRLDQILLTFLIIIALIGFGLVYTVNYQANPAVY
                     LQSPSTSPSSHFFKGIYILFIGALCIYLQNYMIRFIGKQLRIAAEKAASDLRVRIEEE
                     RTAHALQLIEQERLLNKRLENEVKERTLELTQANTRLIELQKENLQSQFEVLKQQVNP
                     HFLFNSLNVLLSLIKTDSDLAESFTEKLSKVYRYVLENKEKDIVPLGTELEFINSYVF
                     LLNIRFTGKIIVRIQTGDDKNGRMILPMALQILIENAIKHNAFSKTNPLRIDISVDKD
                     DFLAVKNNIQARENHIQSTGIGLTNIRNRYKLISDRIPVFEKTETEYIARIPLIKEE"
     gene            complement(5961..6920)
                     /locus_tag="PLZ75_03130"
     CDS             complement(5961..6920)
                     /locus_tag="PLZ75_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71425.1"
                     /translation="MTNRLILTLSLTLMLQACITWPHRDKLIFTEAKPAAGFNYPYFL
                     FVPAGVSDKDEMVLVVEPNNTGAADDDFEKHVEKARRTASRDFYTGNYVARKLNYPLL
                     VPVFPRPLIEWKIYTHAYDRDVALQKNNPLERIDLQLLAMIGDAGIRLGEMGCRINEK
                     VFMTGFSASGSFTNRFTAIHPEKIMAAAAGGTNGLLILPVDSLKGKPVPFPLGTFDFM
                     ELFGRHFDPALFEQTPQYYYIGELDDNDAVSYEDGYDQPERLLVRELLGEEMQPERWN
                     ECIRIYREKKVDVQFRTYKGTGHEVTDEIRTDILDFFRQQAER"
     gene            7684..8811
                     /locus_tag="PLZ75_03135"
     CDS             7684..8811
                     /locus_tag="PLZ75_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71426.1"
                     /translation="MRIAFFLIILIHALIHLLGFLKGFEIKEIKEITSPVSRTMGVIW
                     LTSFFLMLVFGILFLLNYGYSWIPGFIAALISQILVILFWSDARFGTIPNILILAVSL
                     FSFGYFNFNRLIQQDTSQILGQNQVVAEKIINDGDIQHLPEPVRNWLYNSGMVGKPLI
                     SVGKVTQEAEMKMKPGQEKWMKAEATQYTALNVPAFIWTVELKINRLLQIQGRDKFAD
                     GKGDMLIKMNSLINIVNELGEKIDEGALQRYLGEMVWFPSLALSPYITWEKIDSATAR
                     AYMTFKGTSGSGIFYFNSSGDVISYYALRYKDNFAEAKRYGWKMNITEYKTFEGIKVP
                     SKMNSVWELDEGDWTWLNLEVTDIRYNMNSIPGQHISGTRQ"
     gene            complement(9060..10256)
                     /locus_tag="PLZ75_03140"
     CDS             complement(9060..10256)
                     /locus_tag="PLZ75_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS71427.1"
                     /translation="MRSRTFKKGNRNEANTSPHDRRDFLKNVTFLGTAAFVPAVFTQS
                     CKDQKMESSGESENKAAVISQRRILGKGTAAMEVSALGFGVMGMNYHRGIHPDKKAMI
                     KLAHQAVERGVTIFDTAETYGPFTNEELAGEALSGYKDRIAITTKFGFNYAGNRINEM
                     PAGLNSRPERIREMVEASLKRLRIDVIELLYQHRLDPDVPIEDVAGTVKDLITEGKVK
                     RFGLCEVGAETIRRAHSVQPVTAIQSEYHLMWRSVEEEILPVCEELGIGFVPYSPINR
                     GFLTGAINEYTRFDSGNDNRNILPRFTPEAIRANLRIVEELNRFGRTRGMTIAQVALG
                     WLLQKAPYIVPIPGTTKLSHLEENLRTADFSLAKQEWDELEMAVAAIPVTGDRYPASE
                     QRKVEK"
     gene            complement(10622..11275)
                     /locus_tag="PLZ75_03145"
     CDS             complement(10622..11275)
                     /locus_tag="PLZ75_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOS71428.1"
                     /translation="MRVRVKICCISSVSEALTAVRHGADAIGLVGEMPSGPGPIPDSL
                     ISEIAGIVPPPLGTFLLTSETSAAEIVKHLRRTNTNTVQIVDLLSDGSYSQIKDVLPS
                     VKIVQVIHVIDAGSVDLAVRLSESVDALLLDSGNPYLKIKELGGTGRIHDWKLSRQIR
                     DNSKCPVFLAGGLTPDNVKQAIEEVRPFAVDVCSGVRTDGALDENKLIAFMKNSGSL"
     gene            complement(11557..11832)
                     /locus_tag="PLZ75_03150"
     CDS             complement(11557..11832)
                     /locus_tag="PLZ75_03150"
                     /inference="COORDINATES: protein motif:HMM:NF023159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOS71429.1"
                     /translation="MPAAQTIKTLTKIPGVGKSVARDLYNIGIRQIDDLKGRDPEVLY
                     NESNLFAGCIQDRCLLYVFRCAVYFAETPADKQDREKLKWWNWKDKQ"
     gene            complement(11897..12727)
                     /locus_tag="PLZ75_03155"
     CDS             complement(11897..12727)
                     /locus_tag="PLZ75_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOS71430.1"
                     /translation="MKNTVLVITILATLLSGCQGQKSKQTQKELADGKITVITGKQSG
                     YSPASETSATNASLSLDKNEIRSLAQNVKALDPDLPTIGILIFDQVLMTDITAPIDVF
                     AKHSEDGKQLFNVITIAERYDLFTSEEGLKMFPDYTISNAPKLDIIIVPSAYDMTSQV
                     ANKNIVEFIREQNKNTDYTVSNCAGATLIGEAGIADGRRIVTWIGGGADLQKNYPNLK
                     VQDDSKVSFVEDGKFMSSNGNLATYISSLELLEKLTNREHRKFVESHLYLDRLQQWKK
                     "
     gene            complement(12921..13103)
                     /locus_tag="PLZ75_03160"
     CDS             complement(12921..13103)
                     /locus_tag="PLZ75_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71431.1"
                     /translation="MAGGNDVKILDKYIHDVPGGAGIQLGPDGIPFTADDGLRLQSVS
                     PAIGSAPGNRNLGAYQ"
     gene            complement(13082..13303)
                     /locus_tag="PLZ75_03165"
     CDS             complement(13082..13303)
                     /locus_tag="PLZ75_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71432.1"
                     /translation="MVCVKNGTYDERVVIKKGGENDKPIVYVGFPRRETFVSHGFEIR
                     AGHVELIGFDITHDKAGWNENGIWLAVTM"
     gene            complement(13739..14434)
                     /locus_tag="PLZ75_03170"
     CDS             complement(13739..14434)
                     /locus_tag="PLZ75_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71433.1"
                     /translation="MTIDIRFNVYSDAKSGDPDSTSPTLRSYHKLLWSKQLPNGEYFE
                     LTDKKRGAYLFHKSRLGEYYLGSDAITHSYRNHKRKIWLTRQIPNEVEELFDTGSTIG
                     AYTLFPNKRVDGKHTINQARGVNSFIDDRFDLTLECIRLFYIGQQNPLYDTFLRYKDF
                     FDLFENFNGYINFFLLNDLIDDNGKIQFYLPFDNFKTKPAFADVGEYLIYKKGVMDFV
                     KARNKRINKYANS"
     gene            complement(14598..15002)
                     /locus_tag="PLZ75_03175"
     CDS             complement(14598..15002)
                     /locus_tag="PLZ75_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71434.1"
                     /translation="MEYLEEIKYRIDEILVKQKASDDVERLLSKFHYDLIARLNWKTR
                     KIIGRTHAVGITYFLSDHYKAILFLNVRQQYLTAIFFTGDKIIRGLGKGNWVYKNDNK
                     GSETFKIENEFTLNEALNYSLEAFNIATEWSG"
     gene            15326..>15564
                     /locus_tag="PLZ75_03180"
     CDS             15326..>15564
                     /locus_tag="PLZ75_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71435.1"
                     /translation="MYKTCGLDVHKDTVFCGIFDGKRHQVVKEYSTLTSSITSLGNYL
                     RSEGVEDVALESTGMYWMEKTVILTPQIGHVDPLR"
BASE COUNT         4221 a         3475 c         3136 g         4732 t
ORIGIN      
        1 tcaaatgcaa gaccataaaa tccgtggata tcagcccgtg gcatatacaa ttcattaata
       61 ctctgagtgt ttattacttt ccggtctgca acggaatcgt tagtcattcc ggcagcaaga
      121 aaagctgcaa tatcggccac acttgcaaag agaccgccag atgccttctc cgcataaaca
      181 tacaccggaa cgggattccc tttaagatca taacctttag ggatctggtc attgaagctc
      241 tcattccagt caaaactgga atgctccatg cccagaggaa gcagaagctc atttttcatg
      301 tattctgaaa aatcgcgttc agtaatctcc tcaataagtg cctcaagaat gttaaacccg
      361 gtattggagt agctgaatga ccttcctggt tcctgcttaa gaaaagcatc tgccagcaac
      421 tgttgcctca atgcaggctt tttttcgccc ggggcataac gaagccctat ggaccccaga
      481 ggcatgcctg cagtatggct gaggagcatt cttacagtga cagattcttc tgaatattca
      541 gaatccggga tcacccggtt tttaaaataa gtcatcaccg gctggtccgg gtcaagcctg
      601 ccctgttcaa tcagcttcat cactccccac gctgttaagg attttgataa ggactcaaca
      661 cggtaatagg agtcatttgt cattttccgt ccggcttgaa tgtcagcata tccgtaggaa
      721 tcactccaca gaatccggcc ctttttaaga actgacaggc tgactcccgg aattttataa
      781 tgcctcatca gttcaggtac acgcctgtca aggtaattcc tgaatgaatc tgcttcaaag
      841 ctgtatctgc cgggaaccgg tatctttgcc gctgtatcag actgcagtga aaaagaaaca
      901 aacatgaagg ttcccagaac aatatttcgt atcactgaca tagtttttta cacgtttctg
      961 cactgttcag tcataatatg aagtcatcta aaagcagatt ctgtaggtaa ccataggcat
     1021 aaagcccatt tggaaggatg ttgatatttc tttggtgctg ctgttccagt tttgtgtgaa
     1081 tatgttttta tgattggtaa tattttgcag atccaaagcc cattcctgat tcagccggtt
     1141 catattcagg cgaaatgtta ttcttccgtt catcctgaaa taattgggaa aatgcatttc
     1201 ataagcccga tcccatcgat atctgagagt attctctgcc cgtgtgcgtt ccgcatcaat
     1261 ctcaagataa gggctgcctc cggcgtatac gatttttata tctgccgata acagcgattt
     1321 tcttcccagt ttccattcat aaccggacag tgcattaaat acaaacctgt tattaaatgc
     1381 cgagttgcgc tgaactccgt cgtaccctct gtatcgggca tcaaacagcg acactgtaag
     1441 cagatagtaa aatccttgct gaaggaattt ttcaagcgtg atttccatac cgtaattccg
     1501 acccgtgccg gtgttttcca tactgtcaag aaccacaaag gaaaaccctc caccctgatt
     1561 gataagtgaa tattcaggct gatgtttcga aaccggtata ttatacagtt gctgataata
     1621 aagttctgct ttcagtctgt gcccctctcc aaagagacgg tcatatcctg ctacaagatg
     1681 gatacttctt gacagatcaa gatgttcatt ggttttttca taatgcccgg gaaaagcgga
     1741 aaccatattt tgactgaaat acactgcctt catctgggtc tggctgtgaa gcccggctcc
     1801 gagattaaat gactgaccgg gggcattatt ccatttcagc ccgaatcgtg gctcaatgga
     1861 tttgctgtca tttagaaaga agtaagatgt atttaaacct gtaccaaggc taagtttatc
     1921 tgaaaaccgg tgctgccact cgctgaagag acgggcatac cctgtattcc cttctgaatt
     1981 catatagtag aagtaatcgc cggtggtttt gcggaattgg gctccagtat acttcagacc
     2041 aaaaatgtca ataaccaggc cggtgttgag ataattccgc gaattgaacc gctggtggta
     2101 tttagtcgaa aaagtatatt taatttcatg catattttca tctatcactt tctgctgcaa
     2161 tcgcccgtca agatcaaaga tttccgcgat tccttcaatc cccgagactg caattgttcc
     2221 tgtcagacgg gcattatcag tcagatatct gacataactt aatccgccaa cacccatcct
     2281 gttccgataa aagagatctg ttccggagaa tccatattgt gcatcatctc cttcactgtc
     2341 aagcatggcg attctgttat caccgcccag tgcaaacaat gacagacgtc ctttatttag
     2401 cgggaaattc agtttcaatg acagatcctt gtattttggt attgcggagc cggtgccaaa
     2461 actcatcccg acagcatgca gaagttcaag tgtgctgtag cggaagctga gcagataaga
     2521 agccctgctt ttttcatgaa gcggtccttc agctcccagt tcaaacccgt taaacccgat
     2581 ctggcccaga aattcccttt tctggttgtt gccgttacgc atccgcagat caaatactcc
     2641 ggacagtgca ttgccgtact cggcaggaaa tgcagaggta tagaagtcag agttggcaag
     2701 gagattattg tttaacatac ttaccggacc tcctgtggaa ccgagcgatc cgaaatggtt
     2761 cgggtttgga atctccaccc cctcaagtcg ccagagaagg cccagggggg aattcccgcg
     2821 gataataata tcgttgcgct ggtcagagac agaagagact cccgcaaaat ttgttgccat
     2881 acgcgaggga tcgcccaggc tgccggcata tctttctgtt tcatcgatag taaacgaacg
     2941 ggcactgatc accgcaattt cgtttgaagg ctgatctttc cgcgtatttg gccgtattac
     3001 aatttcctcc agtgaatata cctgctctgt cagcctgatt tcaacaatca gttcacgtcc
     3061 tgatgtcaac aacaggttag tcagcacctc cggcttatag cctatcgaac tgacagataa
     3121 ggttatcctt cctacaggca ggttcccgaa gcggaaaaaa ccctctgcgt ccgtgacaac
     3181 cccttttaaa ggatcagaat ccttaataat gataacaaca ccgggtatgg gaacctcggt
     3241 ataagcgtca gtaactcgcc ccctgacagt ctgtacaagc tcctgtgatg gtgaggtcag
     3301 ggagttcaga accagaagaa gggcaatcag caatgatttg attattcttt tcatcagcac
     3361 tcattttaat tttaactact ttaaccaaca gccaaaatta gatcaggcaa atgagtgctt
     3421 aaaatttgtg tggatgaatg acttaaaccg gctcatgaat gccggaaaat tcagttctga
     3481 ccgcctgaag cctattggtc aagccatttt ttaaagcttg ttaccttatc aagacttaca
     3541 aaaacatcct catgaaattt cggattcaga tcaagtttca gacggctttt gggatacaca
     3601 tgtatttcac caataccatt tatcccaacc agtattttcc ggttaatcct gaaaaatttc
     3661 ttcggatcca gttctgccat cagcctgtcg agactgatat cgaccgggta agaacccttt
     3721 tccttcaggt aagcaaatac ccctccccct tcgctgacga aataggccac ctgatcaacg
     3781 gctatggttt ttatttttgt gccgaaactg gcagaaaacc tttcacggta tcggggtgtt
     3841 ctctctgcca tcaaacgata aagtgcatta atgtcggttc ctgcccctgt ctgcccggtc
     3901 attccgctgt attttttaag tgcggccgcc agctcctcct gaataattgg cttgaggaga
     3961 taatcgatac tgttaagctt gaaggccttg attgcgtatt catcaaatgc cgttgtgaaa
     4021 atgattggac atgagatgtt tactttttca aaaatagaaa agctaagatc atcttcaagg
     4081 tgaatgtcaa gaaaaataag atccggctcc ttgttgctcc tgaaccattt caccgactcc
     4141 tcaaccgatt ccagctcggc gataacttca atactgctgt catagtcatg tatcagcttt
     4201 ttaagcctca acgaggcaaa tgattcatct tcaataatta caactttcat gaataaatac
     4261 tgttaatttt aacggctcat ttctttttcc tgtcctggca ggtgtgcaaa tcctttatat
     4321 atacccccgt tttcagcgga ataaataaat ccggatattc cttccggctt attcgttgcc
     4381 ttctcatgct ttggcatgtt attgccaggt cattcctctt ttatcagggg tattcttgca
     4441 atatactctg tttctgtttt ttcaaaaaca ggaatcctgt ccgatattaa tttatatctg
     4501 tttctgatat ttgtaagtcc gattccggtt gactggatat gattctcccg ggcctgtatg
     4561 ttgtttttaa ccgcaagaaa gtcatctttg tcaacactga tatcaatcct gagtggattt
     4621 gttttggaga atgcattatg cttaattgca ttttcaatca aaatctgtaa tgccatcggg
     4681 agaatcatcc tgccgttttt atcatctcct gtctggattc tcacaattat cttcccggtg
     4741 aaccttatat tcagcagaaa gacatatgaa tttataaatt cgagttcggt tcccaatggt
     4801 acgatatcct tttctttgtt ttccaggaca taccggtaga ccttcgacag cttttcagta
     4861 aatgattctg ccaggtctga gtctgtcttt ataagggaga gcagtacatt caggctgtta
     4921 aaaagaaaat gaggatttac ctgctgcttc aacacctcaa actgtgattg caggttttct
     4981 ttctggagtt cgataagacg cgtgtttgcc tgagtcagtt cgagagttct ttcctttacc
     5041 tcattctcca gtcttttatt cagcagacgt tcctgctcaa tcagctgaag ggcatgtgcg
     5101 gttctctcct cttctatcct tacccggaga tcggaggcag ctttttcagc cgcaatcctg
     5161 agctgtttgc cgataaaacg gatcatgtaa ttttgtaaat aaatgcataa ggcccctatg
     5221 aaaagtatat atatcccttt aaagaaatgc gaggagggag aagttgacgg actttgaaga
     5281 taaactgctg gatttgcctg ataattaact gtataaacca gaccgaaccc aatcagggca
     5341 ataatgatca gaaaggtcag caggatctgg tcaagcctga atgtatacag taaaaacaga
     5401 agtggtatta aaaaaatagt tgcagcagag gaggaagcca ggggatcgaa ttcggctgag
     5461 agtatatagt agtgggcaca aatgacaccg acatcgagca gggcacttgc aaaaccaaga
     5521 tatcccggat tatattttct gaccacagca taccacaaca caatattgga aagaaaatat
     5581 accatgatca gaataaaagc atgaacagat tctttccggt acccgagtga aagctgcaca
     5641 attagcagaa cggctaccag gaaaataaaa gtccagcgga agtaaacaga aatccgggct
     5701 cctttcagct gctcttccct gatcagttca cgatcaagat ccatatgcca gtttttcatt
     5761 tttctcctgt ttatagttac accatctctg taaaaaatat tcaaaactca ttaataagtc
     5821 ttactagttc aggcagaatg ccgtacgcaa ttgtaacaat tgttacacat aaccagaaat
     5881 gccttatttt caataattat cattcggctt tgattaattc cggttcttat gggttttgct
     5941 gctgttttcc ctggccacaa tcaccgttca gcttgttgcc tgaaaaaatc cagaatgtct
     6001 gttctgatct catctgtgac ttcatgccct gttcccttat atgttctgaa ttgtacatcc
     6061 accttttttt cgcggtagat cctgatgcat tcattccatc gttccggttg catttcttca
     6121 cccagtaatt cccggaccag tagcctttcc ggctggtcat aaccatcttc ataggaaact
     6181 gcatcattgt catccagttc accgatataa taatactggg gcgtctgttc aaacaaagcc
     6241 gggtcaaaat gccgtccgaa aagttccata aagtcgaaag ttccaagggg gaagggaacg
     6301 ggctttcctt tcaggctgtc aaccggcaga atcagtaatc cgttggtacc tcccgcggcg
     6361 gcagccatga tcttttccgg atgtatggca gtgaacctgt ttgtaaaaga acctgaggct
     6421 gaaaaaccgg tcataaatac tttttcatta attctgcagc ccatctcccc cagcctgatc
     6481 cctgcatccc cgatcatggc aagaagctgc aagtcgattc gttccagagg attattcttc
     6541 tgaagcgcaa catctctgtc gtacgcatga gtgtatattt tccattcgat gagcgggcgg
     6601 gggaaaaccg gtacaaggag gggataattc agcttccgcg caacatagtt gcccgtataa
     6661 aaatcgcgtg atgccgttct cctagctttt tccacatgtt tctcgaaatc atcatctgca
     6721 gcacctgtgt tattaggttc aacaaccagt accatttcat ccttatccga tactccggca
     6781 ggaacgaaaa ggaaataggg ataattgaaa cctgccgcag gttttgcttc agtaaaaatc
     6841 agtttgtccc tgtgaggcca tgtaatacag gcttgtaaca tcagggtcag ggacagggtt
     6901 aagatcagac ggtttgtcat atgtaaaaag ttataatgtt ttcaggtaaa tttatccctg
     6961 aagacatcct gatctgccag gttgtccgca ggttttagca gtatcaggat gaccacaggc
     7021 gaatgatcat ttttaatact tcttttcaca actattgaat tataaataac tctcctgacg
     7081 gaagagtggt ttttcttcct ttttgtgtca ctgtttcaat cgggtttttc gccgaaaata
     7141 atatcacctg ctgccaggta caaaaagaag aagacgaaag ggtggtttcg ctggttgaat
     7201 gacctgtaac cttgattttt ctctttattc atgatctttc aggctaagcc gcctggcctg
     7261 attgcttaac tgaatgcttc tgcagaaaca taccctccct ttattgtttc tcggatgctc
     7321 ctgtaaagat aaagcataat tgccggagtg acattcaggg taataatgaa taaagtattc
     7381 accggctccc ggctttgcaa aacacagcat aacaaccggt aagccataaa ttacataaat
     7441 tatttttttt gatttttggt aattattaat caactttgtt attcgccagt ccgttatcag
     7501 accctctaat taccaggatt gtacgagagc ctgacaatat taacctgaac ctactgaaga
     7561 tcctgttatg acaaagtttc aggtttaaaa acatttgcag aggtctgcgg ggatcagtcg
     7621 ttttacacct ctgaagcgtt tgcagcaggc attagggttt gttacattat acaattcgtt
     7681 gaaatgagaa tagcgttttt tttaataatt ctgatccatg cactgatcca tttacttgga
     7741 tttttaaaag gatttgaaat taaagagatt aaagaaatta cctcccctgt ttcaaggaca
     7801 atgggagtaa tatggcttac ttcttttttc ttaatgcttg tgtttggcat tttgttttta
     7861 ctcaattacg ggtacagctg gatacctggt ttcattgccg cattgatttc acagattctt
     7921 gtcatacttt tctggagtga tgccagattc gggactatac ccaatattct cattcttgct
     7981 gtgtcactgt tttcattcgg atatttcaat ttcaaccggc ttattcaaca ggataccagc
     8041 caaattttag gtcagaatca ggtagttgcg gagaaaatta tcaatgacgg tgacattcag
     8101 catttgccgg agcctgtcag gaactggctt tacaattcag gcatggttgg aaaaccactt
     8161 ataagtgttg gaaaagtaac acaggaagct gaaatgaaaa tgaaaccggg acaggaaaaa
     8221 tggatgaaag ctgaagcaac ccaatatacg gctctcaacg ttccggcctt tatctggaca
     8281 gtcgaattga aaattaacag gctgcttcaa attcagggca gggacaaatt tgcagacggc
     8341 aaaggggata tgttgattaa aatgaattca ttaatcaaca ttgtaaatga attgggtgag
     8401 aaaatagacg aaggggcttt gcaacgatac ctgggtgaga tggtatggtt cccttcactg
     8461 gcattaagtc cttacatcac atgggaaaaa atagactccg caactgccag ggcatatatg
     8521 acttttaaag gaacttcagg gagtggaatc ttttatttta actccagcgg tgatgttata
     8581 agttattatg ctctgcgcta caaagacaat tttgcggaag ctaaacgata tggatggaaa
     8641 atgaacatta cggagtacaa gacttttgaa ggaataaaag taccatcaaa aatgaattca
     8701 gtctgggagc ttgatgaagg cgactggact tggttgaatc ttgaggtgac cgatataaga
     8761 tataacatga attcaatacc aggacaacat ataagcggca ccaggcagta aagtgcatta
     8821 cctgtcagat caggtattgg ctattatatg tcggtatctc tgattctttc acatacggcc
     8881 tgctgttgtt tccaatgccg cgccaggcaa tggtccccgc tgagaacggt cttaccggag
     8941 cctgctctga acggaaattc agtacgccag ggcagaacca gattgcgttt tgcaatctgc
     9001 ttcaatccgg cattacaggc tgtaagtttt atcagaccat aatttacttt tctgcatttc
     9061 tacttttcta cttttctttg ttcagatgca ggataacggt cgcctgtaac gggaatggca
     9121 gcaaccgcca tttcaagctc atcccattcc tgttttgcca gactaaaatc cgcggtcctc
     9181 aggttttctt ccagatgtga aagcttggtt gtcccgggaa tgggaacgat atatggtgct
     9241 ttttgcaaca gccagcccag ggcaacctgt gcaatagtca tcccccttgt tcttccgaat
     9301 ctgttcaatt cctcaacaat tctaagatta gcacgtatcg cctctggtgt aaaccgtggt
     9361 aaaatattac ggttgtcatt gccggaatca aaccttgtgt attcgttgat ggctcctgtg
     9421 agaaaaccgc ggtttatggg actgtaaggt acaaaaccaa ttcccagctc ttcacataca
     9481 ggcaatattt cttcttccac actacgccac atcaaatggt actcgctctg aatggctgta
     9541 acaggctgca ctgaatgtgc ccttcttatt gtttccgccc ctacctcaca aagtccgaaa
     9601 cgttttactt tcccctctgt tattaaatct ttaaccgtac ctgcaacatc ttcaatgggt
     9661 acatcaggat ccaggcggtg ctggtacagc agttctatca cgtcaatcct gagacgtttc
     9721 agggacgctt ccaccatttc acggatacgt tcgggacggc tgttcagtcc tgcgggcatt
     9781 tcattgattc tgtttccggc ataattgaag ccgaattttg tggttatggc aatcctgtct
     9841 ttataaccgc tcagggcctc acctgccagt tcctcatttg tgaacggacc gtaggtttct
     9901 gccgtatcaa agatagtaac tccgcgttcc acagcctggt gggccagttt tatcatcgct
     9961 tttttatcgg gatgtatgcc acgatggtaa ttcattccca ttacaccgaa gcccaaagcc
    10021 gatacttcca tggcagctgt tcccttcccc agaatacggc gctgtgaaat aacagctgct
    10081 ttgttttcac tctcgcctga ggattccatc ttttgatctt tacagctttg agtgaaaaca
    10141 gcaggaacaa atgcggctgt tcctaaaaag gtcacatttt tcagaaagtc acgtctgtca
    10201 tgtgggcttg tattagcctc gttccggttt ccttttttaa atgttctgct tctcataaat
    10261 atattttttt gtgtggttcc gtccgaacca gctctggccg gtagttaagc cctggtctgc
    10321 ggaccggatt gtatccggtg gcctgtctgg catgtagcga ccacatacag caaaaagttg
    10381 attgtttctg taaagataag aagcaatttg gtttaacaca ccttccgggc acattacttc
    10441 cggaggcagt agctgcaagt ccggatacat ccggaattca gctccggctg aatatgtctg
    10501 gacctggccg ggaatgaatc tgtgtcttaa cagcctgaat gtccctgaaa ttttttatca
    10561 gccgtagtta cttttttctg tactagccga agaagttttt aaaatggaga gcagactttt
    10621 ttcaaagact tccggaattt ttcatgaatg caattaattt attctcatcc agggcaccgt
    10681 cagtcctgac tccactgcaa acatcaacag caaacggccg gacttcctca atggcctgtt
    10741 ttacattatc cggggttaat ccaccggcaa ggaaaacagg gcactttgaa ttatcacgga
    10801 tctgtctgct aagcttccag tcgtgaattc tgccggtacc gccaagttct tttattttaa
    10861 ggtaggggtt tccgctatcc agcagtaagg catccacact ttctgaaagc ctgacagcaa
    10921 gatcgaccga tccggcgtca ataacatgaa ttacctgtac aattttcacg gatggcagca
    10981 catctttaat ttgcgaataa gacccatcag aaagcagatc gacaatctga acagtatttg
    11041 tgttggtcct cctgagatgt tttacaatct cagcggcaga tgtctcactg gtcagaagga
    11101 atgtaccgag cggaggcgga acaattccgg caatctcgct gatcagactg tccgggattg
    11161 gtcccggacc gcttggcatt tcaccgacca gtccgattgc atccgcccca tgtctgacag
    11221 ctgtcagtgc ttcggaaaca ctgctgatac agcagatttt tacacgtact ctcataaaaa
    11281 gtttttattt tgaagcaacg cccttcttac agccaacggt ttcagaacgg ctatcctata
    11341 gtttccggga aatgttttct gaacaattgt acaaataacg aataaaagcc aactgcatta
    11401 tttgttattg tccgggttat aattgccgga ttatagttgc cggaataagc ttgccggaac
    11461 aagtctgccg ggctactgta caaatacccg gctgaagaaa aacgttatac ctgactattg
    11521 caaagactat ttttggaaga atggttttat cagcaattat tgtttgtctt tccagttcca
    11581 ccatttcaat ttttccctgt cctgtttgtc tgccggtgtt tctgcaaaat atacggcaca
    11641 cctgaagaca taaagcaaac acctgtcctg aatgcacccg gcaaacaagt tggattcgtt
    11701 atacagcact tccggatctc tgcctttcaa atcatcaatt tgtctgattc cgatgttgta
    11761 taaatctctc gcaacagatt ttccgacccc cgggattttt gtcaatgttt ttatagtctg
    11821 cgctgcaggc attatagttt taaatcatcc tttccgcgga tactatccgg aatgatatat
    11881 tgtcagggca atatatttat tttttccatt gctgcaatct gtccagatac aaatgtgact
    11941 ctacaaattt cctgtgttcc ctgtttgtga gtttttccag cagttccagg gatgaaatgt
    12001 aagtagccag atttccgttt gacgacataa atttgccatc ttccacaaaa ctgacttttg
    12061 agtcatcctg tacctttaaa ttgggataat tcttttgcag atcggcaccc ccgcctatcc
    12121 aggtaacgat tctccttccg tcggcaatac cggcttcgcc gattaatgtt gcaccggcac
    12181 agttacttac agtgtagtca gtatttttat tctgttccct gataaattcc acaatattct
    12241 tattggctac ctggcttgtc atatcatatg cactcggaac aattattata tccagtttgg
    12301 gtgcattgga aatggtatag tccggaaaca ttttcaatcc ttcttcactt gtaaaaaggt
    12361 cataacgctc ggcaatcgta atcacattga atagctgctt cccgtcttcc gaatgttttg
    12421 caaaaacgtc tatcggagca gtgatatctg tcatcaggac ctgatcaaat atcagaatac
    12481 caatggttgg cagatcggga tccagtgctt taacattttg ggctagtgac cggatttcat
    12541 ttttgtcaag cgaaaggctc gcatttgttg ctgaggtttc tgaagccgga ctgtaacctg
    12601 actgtttacc agttataacg gttatctttc cgtcagccag ctctttttgt gtttgtttgc
    12661 ttttctgtcc ctgacatcct gaaagcaagg ttgccagaat agtgatgacc agtactgtat
    12721 ttttcatttt actcagtatt tcatattatt aatattatat taacagctgg caatatattt
    12781 ttgttgccgg atctgaagca cagccggttt tgttaacctt aagttttctg aaaatcccgg
    12841 atcttatgcc tgatacaggt cgcaggctct tcccggtaac tgcattatcg catgcagtca
    12901 tacagaggta agggccagga ttactggtat gcaccaaggt tccggtttcc cggtgcactg
    12961 cctattgccg gcgaaacgct ttgcagccgc agaccgtcat cagcagtaaa agggattccg
    13021 tcgggaccga gctgtatccc ggcaccgccg ggtacatcgt gaatatattt atccagtatt
    13081 tttacatcgt taccgccagc catattccat tctcattcca cccggccttg tcatgtgtaa
    13141 tatcgaatcc gataagctca acatggccgg ccctgatttc aaaaccatgg ctgacaaagg
    13201 tttccctccg gggaaagcct acataaacaa tgggtttgtc attctctccc ccttttttga
    13261 ttaccacacg ctcatcatag gtgccgtttt tcacacagac cacatcgccg ggcttaacgc
    13321 tgttggctgc atgctgaatg ctcagccacg gcagttcttt agtaccggga ttatcatcac
    13381 tggcattgct gtgcttgttg tcaatatagt aatcaaagat aaaagcatca taatcaacct
    13441 tcgggactgg ctccgctatc ggaaatccgg cacagatgtt tagtcagatt aattttttgt
    13501 atttagcttt tttgcttccc tgaaactaaa aactgcatct gctttattgt tttctgccag
    13561 tgccgcgact ctgcttccag tctgaactaa tgactttaac atttacaaaa aatgatgtga
    13621 ttctgctaac aggatctcaa atactatttc caacgaaaga cagacttcag gtttttagca
    13681 ccaaaagatc aagcatctta gcagtcggcg gtaagtggct ggtattctat tttgttctct
    13741 aagagtttgc atatttgtta attcgtttat tacgtgcttt aacaaagtcc attaccccct
    13801 ttttataaat taaatactcg ccaacatcgg caaatgccgg ctttgttttg aagttgtcaa
    13861 atggcaagta aaactgtatt tttccattat cgtcaataag gtcgttaagt aaaaagaaat
    13921 tgatatatcc attaaagttc tcaaacaagt caaagaagtc tttgtatctt aaaaatgtat
    13981 cataaagtgg gttttgctgt cctatataaa aaagtctaat acactctaaa gtcagatcaa
    14041 acctgtcgtc tatgaaactg tttactcccc tagcttgatt tatggtgtgt tttccatcaa
    14101 cacgcttgtt tgggaaaagc gtgtaggctc caatcgttga acctgtatcg aatagctctt
    14161 ccacttcgtt tggaatctgt cgtgtcagcc agatcttgcg tttgtgattt ctgtatgaat
    14221 gagtaattgc atcacttcct aaataatatt ctccaagtct tgatttgtgg aataaatagg
    14281 cccctctttt cttatctgtc aactcaaaat actctccatt aggcaattgt ttgctccata
    14341 acaatttatg ataggagcgt agcgtcggac ttgtgctgtc cggatcgcca ctttttgcat
    14401 ctgaatatac attaaatcta atgtcaattg tcattattta tcttatgata ctttttactt
    14461 gatcacatct ctacgctggc caccaacttg aaaatataat caaggatttg ttgagcctcc
    14521 atgatcagta tttaaaatcc cgttcttttc taacttgtgt tagcatgcag aaaaaccgtg
    14581 atcaaatgtt taatatatta acctgaccac tcagtggcaa tattaaaagc ttctaacgaa
    14641 taattcaaag cctcatttaa cgtaaattca ttttcaatct tgaaggtttc actgcctttg
    14701 ttatcatttt tataaaccca gtttcctttc ccaaggcctc taatgatttt gtctccggtg
    14761 aaaaatattg ccgttaaata ctgttgcctg acgttcaaga aaagaattgc tttgtaatga
    14821 tcggataaaa aataagttat tcctacagca tgtgttctgc caataatctt cctggttttc
    14881 cagttcagcc tagctatcaa atcatagtga aattttgata aaagccgttc tacatcatca
    14941 cttgctttct gctttacaag tatctcatca atacgatact ttatttcttc caaatattcc
    15001 ataattctaa tataaatggt ttatagacac ttaaaagcgg atattcttat ttgccaacgt
    15061 cactgtaact gagttctgca caaaggtcaa ttccgccagt gtttgccctg gtcggaagta
    15121 aaatctaaaa aggtgtggaa ccaatctacg tttatcattc tgccgggctg tgtttaggta
    15181 caatatccgg caagaattga atatttatat aatatacatg aaaaatctcg attgacacac
    15241 agacgaataa aaatccagtc cgtgttttga agctctttcg tgttcgagac acattcgtat
    15301 attagagcaa ataaatttca atattatgta caaaacatgc ggactggacg tacacaaaga
    15361 tacagttttt tgtggcattt ttgatggcaa gcggcatcag gtagtaaaag aatattccac
    15421 tttgacatca tcaattactt ctcttggcaa ttatttgaga agcgaaggag tggaggatgt
    15481 tgctttagag agtaccggga tgtactggat ggaaaaaacg gtgatattga cccctcaaat
    15541 cggtcatgtt gaccccctga ggtt
//
LOCUS       DAOSPX010000036        15176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4007_length_15176_cov_4.355532, whole genome shotgun sequence.
ACCESSION   DAOSPX010000036 DAOSPX010000000
VERSION     DAOSPX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15176
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4007_length_15176_cov_4.355532"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..539)
                     /locus_tag="PLZ75_03185"
     CDS             complement(69..539)
                     /locus_tag="PLZ75_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71436.1"
                     /translation="MGKNHASPPGVGHANKTYRQPLWPIKTKTMKQFSKLIYLAILIV
                     LGSCINNNEVRIFIENQSKISDSLVIRDLITEDPIVILPYTVSDTLIKINEPIVVSIA
                     SLSNSDNNLHLGILSPNSDKSLKIDSSGNLKSNYLADSLLQYLFISKCDKKSHK"
     gene            complement(720..1223)
                     /locus_tag="PLZ75_03190"
     CDS             complement(720..1223)
                     /locus_tag="PLZ75_03190"
                     /inference="COORDINATES: protein motif:HMM:NF027625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4943 family protein"
                     /protein_id="HOS71437.1"
                     /translation="MKILLRSFILVTLFGLLGCEKHTDNPDVDTYIDQLISGTYESFD
                     LPEFNTADISALLEYRNETTIITNFPRNPISSFWQQECKLGMFVLWTVESIRAVETNS
                     KFLIGRFPSQNPVLALRDAPELQIVFDDQSHKKAASAYYDWWNSIHLFKDKIKIDPLG
                     KTKYKWH"
     gene            complement(1387..1536)
                     /locus_tag="PLZ75_03195"
     CDS             complement(1387..1536)
                     /locus_tag="PLZ75_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71438.1"
                     /translation="MIRLFLQEAGKRPVGEINNDHVVDNIYRHVVDKGYSSAYKPLIF
                     RLYKI"
     gene            1727..3316
                     /gene="istA"
                     /locus_tag="PLZ75_03200"
     CDS             1727..3316
                     /gene="istA"
                     /locus_tag="PLZ75_03200"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOS71439.1"
                     /translation="MIKKTEMDARQKRLIIMYHEIQRLRNVEHFSIQRIADKLKINFR
                     TVRKILHMTEEDYDKFIEKKWAKGRLLDPYRDFIISYLQQYPDTPSAVMHDRLKERFD
                     TLPRVDPKTVYNYVMTVRRDHNIHMVSSSERQYSAVPDLPAGHLAQVDFGEKKLRRST
                     GEWVKVYFFVIVLCYSRHKFVLFLDRPFTSDDAVIAHEKAFEFFGGIPREIIYDQDSV
                     FLHRENGGDYLMTDVFHRYQASRGFKVTFCRAGDPESKGKVENSVKFVKHNFLFNRTF
                     INHNILNDQVLAWLERTGNGTLHNTTRKVPQEQWLLERPFLRRWIPLLTAARTNGHKV
                     IKTNTIKYRGNSYSLPFGTYRNDESRVFVTEEGNELVIKNAAGSIITTHLIPDGVGHN
                     IINTNHRRDTSIKLDVLRGKVREFFGHSGEIDTFIDKINKLYPRYVRDQLTAVLTAAE
                     RSGQQHAGIALEFCVRNSLFSASDFKSVLQGQKAGRTHLSTVARPVIKPLGDARTQLI
                     VNIEPERSDIREYEVIFNQSL"
     gene            3321..4067
                     /gene="istB"
                     /locus_tag="PLZ75_03205"
     CDS             3321..4067
                     /gene="istB"
                     /locus_tag="PLZ75_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOS71440.1"
                     /translation="MSQYIPLIRRQASRLRLSGIGGNIPNLIREAEQNSPSYDEFTHS
                     LFAFEIKGREAKQLMMRTKLARLPLNHDLDSYDFNFSSGLSASQLNQLRELNWLDQCY
                     NIMFSGPSGTGKTYISAGLCYDALQRGYKAYFRGMNDILATLRLKELTPTAGKEFKRL
                     SEAQLIVIDDVMTLPLGREDGNRFFVFLNQIYETTSFIITTNKSPAEWARSVDDETLA
                     TALLDRLLYKCQLIQLQGKSYRMQNRKTIF"
     gene            4570..>5234
                     /locus_tag="PLZ75_03210"
     CDS             4570..>5234
                     /locus_tag="PLZ75_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS71441.1"
                     /translation="MAKKFSSKIAIKDRTTNQSLTFSKALIGALILTSKFKKYDKGFI
                     GIMIPTSAGCALASVAALMSGRIPVMINYSTGAEANARYAQKKCRFNTIIASKALLDK
                     IGCPVIEGMVMIEDLMKGVTTADKLKAALKSMLPVKLLLNTIHKGAEDDYVVMLFTSG
                     SEKDPKAVPLTHRNIVSNIESFTKVISITSSDIILSNLVFYHVFGLTVDLWVPLYLGM
                     TMIT"
     assembly_gap    5235..5244
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5300..6103
                     /locus_tag="PLZ75_03215"
     CDS             5300..6103
                     /locus_tag="PLZ75_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS71442.1"
                     /translation="MAREERPTLMVGTPSFFWGYLQKSEPGDYKSFRLMVSGADKCPD
                     ALRGEFKKKHGVTLLEGYGTTETSPVISVNSHEFNRPGSTGRVIPGLQVRIENFETGE
                     DCKTGEVGKIMVKGDSVMKGYFEDPEQTAEVLKDGWYNTGDMGFLDGEGYLWHAGRFK
                     RFTKIGGEMVSLVKVEGVLEKLLPEGVKCCVVEISDEKKGSYIVAAVSAGINKTEILR
                     KMMKELPIISLPRQFVVIEELPMMGTGKIDFRRVTQMVGEMEEVTGDAR"
     gene            complement(6137..6487)
                     /locus_tag="PLZ75_03220"
     CDS             complement(6137..6487)
                     /locus_tag="PLZ75_03220"
                     /inference="COORDINATES: protein motif:HMM:NF018088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOS71443.1"
                     /translation="MKIISLDQVDKMQVQMKGASGAWKQLPLGSMDGAPLYSLRVFTI
                     EPGGNTPYHSHPYEHMNYVIEGQGVLVDPEGEEHPMKTGDFALVSPDEKHQYRNTSPD
                     SLFRIICGVPKEFE"
     gene            complement(6509..8035)
                     /locus_tag="PLZ75_03225"
     CDS             complement(6509..8035)
                     /locus_tag="PLZ75_03225"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOS71444.1"
                     /translation="MVILDQIGFDNDKYLEEQTSAILERVNRFHQKLYLEFGGKILYD
                     YHAARVLPGFDPNVKMRLLQMLKDKIDVILCIHAGAIERKKVRADFGITYDVDALKTI
                     DDFREWGIDIAAVVITRYQNQPHAKIFRNKLQHRGVKVYLHYPTKGYPTDVDLIVSDD
                     GYGANEQIKTTRPIVIVTGPGPGSGKLATCLCNLYHDYRKGIKAGYAKFETFPVWDLP
                     ADHPVNLAYEAATADQEDRVLLDDYHMKAYNIKSVNYTRDLEAFHLLKKIIEKITGGE
                     SMYQSPTDMGVNRASAGIIKDSIIREASRQEIIRRFFRCSVEYAAGLSEKEAMDRIVL
                     IMDKVGVREEDRNVVEPARKAARDAEKCGKGNEGIFSGAAIELHDGTIITGKNSPLMQ
                     ASSSLVLNAAKYLAGLPDKMYLLPENIIDSVTHLKKNILHGKMVSLDVDETLIVLGIS
                     ALSNPAAKMALENLKLLRDCEVHLTHIPTPGDEAGWRKLRVNLTCDPVYSSKSLFISA
                     "
     gene            8319..9761
                     /locus_tag="PLZ75_03230"
     CDS             8319..9761
                     /locus_tag="PLZ75_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71445.1"
                     /translation="MKCYKLLLTILPALLILSGCSDTDSSTAKRKKLTKKEINYIVEF
                     DKPVPSIPSGLRISTFDTDATPPPGTWLAYDSLLNTGDLGLRAKGLVILGAGEPIVIC
                     CVDWLGISNAGYDSFRRSLGNAAGTSPERVSIHTVHQHDAPNCDFTVEKILLDAGVDP
                     ACFEGTFHREFIRKLSYVVRNSLEKSQPVTHIGTGRGIVDRVASNRRILGENGKVIGT
                     RWTTTKDPALRALPEGLIDPEVSVVSLWNNDDPLAVLSFYASHPQSYYRTGIANPDFP
                     GIARFFRQLEVPDALHIHFNGAGGNIGAGKYNDGAHENRLILAQRLAEGMKLAWESTV
                     KEEITPESVRWNYIPVSLPPAKHLLELEKRMTEISDTLYSHSLASKLAWLERTRAGIK
                     TDVSCLSAENTRILFLPGELFVEYQLTARSMRKDLFVAMASYGEYGMGYIGTAAAYDE
                     GGYETGDESFVDSGSETILMDAISRLLGNR"
     gene            complement(9794..11149)
                     /locus_tag="PLZ75_03235"
     CDS             complement(9794..11149)
                     /locus_tag="PLZ75_03235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS71446.1"
                     /translation="MKKITRRKFITTASAGSAAVASGAISPFKDIEIINDNSGKLAIL
                     GGTPAAPAKVWPEWPYIDEKVIAEMMDTAKSGIWCRIQSADNKVLKFENEFARLMGIK
                     TSIATGSGTQSLNTCIEALGIGPGDEVITSPYTDPGTIASILVSRALPVLADLDPDSF
                     QLDPDDVERRITSRTKALMPVHMMGQPCNMERIMEIAKKHKLLVIEDSAQAHFAEYQG
                     KKAGLIGDVGCFSFQTSKVLSCGEGGGIVSNNEELLDKCYTVHNHGTSKRGRTETIGS
                     KYRMNEFEAACLLGQLPGVMDRHYIRNRNAKYLTEHLKDCPGLVPQKLYPGTTAGSWY
                     LYPWSYKKDMFNGAPREKFLKAIAAEGISLSPYITNGLHREPWTQHIQTLPVYRKMYG
                     AARLRKFNSELHLPKCDQVCEDMVMLWASGPLLGTLADMDDLINAIMKVYVNRDKLNT
                     I"
     gene            11239..12045
                     /locus_tag="PLZ75_03240"
     CDS             11239..12045
                     /locus_tag="PLZ75_03240"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS71447.1"
                     /translation="MNNRFIGSGIKITAIAISMSMLLISCNTWPGFTGNKTEKINRLT
                     RAEKKQGFMLLFDGKTFNGWRGLGRDHVPAGLWVIEDGMIKKVDTRNKEKLPDGRPVE
                     GGDLMTVETYENFELTFEWKLNKAGNSGVKYNVSEEMSQEYGSGFSALGFEYQLLDDD
                     DESYRGVLKPAQFTGSLYDLIPSRNVALRPVGEFNSSRIIVDGDHAEHWLNGIRVLEY
                     DFGSQELEEAYRDSKFSKIPDFIRKRKAHIVLQNHNDESWFRNIKIRIID"
     gene            complement(12069..12524)
                     /locus_tag="PLZ75_03245"
     CDS             complement(12069..12524)
                     /locus_tag="PLZ75_03245"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HOS71448.1"
                     /translation="MSLNNFTLEKTAKTPRIDLNKDSGELVFSGKSIPENAAKVYEPV
                     LNWVSEYALKAKPITNLRLDIEYFNTASMMWLSRILVILKKINDPEYILYIHLYMPLE
                     EYDDMNDIDDIRDALIPVQDILQDAIPCVGIKLYGTNNSRVIKDALVFI"
     gene            12842..13768
                     /locus_tag="PLZ75_03250"
     CDS             12842..13768
                     /locus_tag="PLZ75_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS71449.1"
                     /translation="MDIVIENLTKRYGVQKAVDNISLRVATGEILGFLGPNGAGKTTT
                     MKIITNFITADEGEVYIGGRSLRDDPLEIKRHIGYLPENNPLYQEMPVIDYLEFCALI
                     NNIEKHRVAGRVAEMIRITGLNSEKHKKIAELSKGYRQRVGLAQAMIHDPEILILDEP
                     TSGLDPNQIAEIRKLIRELGREKTVILSTHILPEVEATCDRIFIINKGRLVADGTAES
                     LRKKAQGSDILRVRIEDGDIDQIFKALQSLKAVSMVDFADRQKNRFDVHCRSEEVKRD
                     IFRLCVERNWVLTEITPFETRLEDIFRELTMN"
     gene            13781..14506
                     /locus_tag="PLZ75_03255"
     CDS             13781..14506
                     /locus_tag="PLZ75_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOS71450.1"
                     /translation="MKTIWIIAKREVNSYFDSLTAVIMIVLFLGFSGFFTWISGSDIF
                     FLGQASLRSFFGIAYWTLFFFIPALTMKTFAEENKSGTIEMLLTKPVTDRQVILGKYF
                     ATLILIAIALALTLPYVITVSKIGNLDAGGTFCGYLALLLISAAYASIGLFTSSITSN
                     QIVAFLSALFIGLFFHIIFQIIAGGMKGFLGQIVNYLSITFHFESLARGVLDSKDVIY
                     FASIIFMGLFLTEVSLSKRNAFN"
     gene            14555..>15176
                     /locus_tag="PLZ75_03260"
     CDS             14555..>15176
                     /locus_tag="PLZ75_03260"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOS71451.1"
                     /translation="MRKAKITSTIIIVTMIVVVINILSKSYNFRLDFTEGKEYTLSKA
                     TRDILEGLDKPVTVTAYFSKDLPPHIGSISDHLRDMLIEYGNRSKGMVVFKFVNPNEQ
                     EDLEQEAMKNGIQPVMINVREKDQVKQQKAYLGAVVSVGDDRETIPFFQPGAAMEYAL
                     TTSIKKLSITDKPAIALIQGHGEPSLNEMPQVYAELSVLYNVEPFTM"
BASE COUNT         4208 a         3203 c         3418 g         4337 t
ORIGIN      
        1 tgccagtcac gcacgcctct ttaggctact attgacggaa caataggttc agacgccatg
       61 ctgaacaatt acttgtgcga cttcttgtcg cacttactaa taaaaagata ctgaagcagc
      121 gaatcagcca aataattact ttttaggtta ccgctggaat cgatttttaa tgatttgtca
      181 ctgttaggag aaaggatacc cagatgaaga ttattatcag aattgcttaa actggcaatg
      241 gaaactacaa tcggttcatt tatttttatc aatgtgtcag atacagtata aggtaaaatt
      301 acaatggggt cttctgtaat taaatctcgt atcacaagtg aatcacttat tttactttga
      361 ttttcaatga atattctgac ttcattgtta ttaatgcagc ttcccaatac tattagaata
      421 gcaaggtaga taagttttga aaattgcttc attgtttttg ttttaattgg ccacaacggc
      481 tggcggtatg ttttgtttgc gtgcccgacg ccaggagggc tggcgtggtt tttgcccatc
      541 gcggcgcttg cagtaatctg tctttttgtt tttcaggtta tcgcgccgct gtcacaagtt
      601 tgctggatgt tacaaatcta tccttctgca catcgtgtta tagacagcga gcaaaaacca
      661 tgacaagcaa atgaaatata ccgacctgtt atgcttaggc ttatttatta ttctcaaggc
      721 taatgccact tatattttgt tttccctagt ggatcaattt taatcttgtc tttgaataaa
      781 tgaattgaat tccaccaatc ataataggcg ctggcagcct ttttatgtga ttggtcatca
      841 aaaactatct gtaattcagg agcatctctt aaggcaagaa caggattttg tgaagggaat
      901 ctacctatta aaaacttact attggtttca actgctctta tggattcaac tgtccacaaa
      961 acaaacatac caagtttaca ttcttgttgc cagaaagagg aaattggatt acggggaaag
     1021 ttagtgatga ttgtagtttc atttctgtat tcaagtaaag cagatatgtc agcggtgtta
     1081 aattctggta aatcaaatga ttcatatgtg cctgaaatta actgatctat atatgtgtct
     1141 acatcaggat tgtctgtatg tttttcacat ccaagcagtc caaataatgt aactaatatg
     1201 aatgatctga gaagtatctt catgggttta gtggttggtt tatcaactta tatgctacaa
     1261 aaactgttcc atttgattta ttaattctct ggacgactta agcttaagca taacttactt
     1321 atttgtatac caaaggtaaa caataattgc tgattcaggt ggttattgtg tggctgggat
     1381 aaatttttat attttgtaca gccggaaaat cagaggctta taggcagaac tgtaaccctt
     1441 gtcaaccaca tgcctgtaga tattatccac cacatgatca ttgtttatct ccccgactgg
     1501 ccgcttacct gcctcttgca ggaataatct gatcatatgg gcataggttc aggtggtacc
     1561 accttaaggt gctagaggcg taagcctctt tgtaatcggg aagcaaaaat gcagtaaaat
     1621 tgggaagtgc aatgcagtaa aaatgggaac aatgatacag taaaatcggg aacaattatg
     1681 cagtgataag ttatcttgat tattaacctg tgctaccttt caggatttga taaaaaagac
     1741 tgagatggat gcacgccaaa aacgacttat cattatgtat catgagattc aaaggctccg
     1801 taatgttgaa catttttcca ttcagcgcat tgcggataaa ttaaaaatca atttccggac
     1861 cgttaggaag atcctccata tgacggaaga agattatgac aaatttatag agaagaagtg
     1921 ggcaaaaggc cgccttctgg atccctaccg tgattttatt atcagctacc ttcagcagta
     1981 tccggatact ccttcggcag tcatgcatga caggcttaag gagcggtttg atacactacc
     2041 tagggttgat cctaaaacag tttacaacta tgtgatgacg gttcgccggg accataacat
     2101 tcatatggtc agttccagtg aaaggcagta ctcggcagtc ccggatcttc ctgcgggcca
     2161 cctggcccag gttgacttcg gagagaagaa gctccgtcga agtaccggtg agtgggttaa
     2221 ggtatacttt ttcgttatag ttctttgtta cagccgtcac aagtttgttc tgtttcttga
     2281 caggcccttt acctctgacg atgctgttat agcccatgag aaggcattcg agttttttgg
     2341 tggcatcccc cgcgagataa tctatgatca ggacagcgtt tttcttcacc gtgaaaacgg
     2401 cggagattat cttatgacag atgtttttca tcgctaccag gccagccggg ggtttaaggt
     2461 taccttttgc cgggctggtg atcccgagag taaaggtaag gttgagaact cggttaagtt
     2521 cgtcaagcac aactttcttt ttaatcgtac attcataaat cataatatcc ttaatgatca
     2581 ggtcctggcc tggcttgaac gtaccggtaa tggcacgctg cacaatacca cccgtaaagt
     2641 gccacaggag caatggcttc ttgagcgtcc ctttctgcgg cgctggatac cactgcttac
     2701 agcagcaaga acaaatggtc ataaggtcat taaaacaaat actataaagt atcggggcaa
     2761 cagttactcg cttcctttcg gtacttaccg caacgatgag tcaagagtat ttgtaacaga
     2821 agaaggtaat gagttggtta taaaaaatgc tgcaggcagc ataataacga ctcatctgat
     2881 cccggatgga gtaggccata atattataaa taccaatcat cgcagggata cttccattaa
     2941 acttgatgtt ctgcgtggca aggtaaggga gttttttggc cactccggtg agatagatac
     3001 atttatcgat aagatcaaca agctttatcc ccgctatgtc cgtgaccagc ttacggcggt
     3061 tcttactgca gcagaaagaa gcgggcagca acatgcaggg atagcccttg agttttgtgt
     3121 gcgtaacagc ctgttcagcg caagtgattt taaatcggtt ctccagggcc agaaggccgg
     3181 cagaacccac ctctctacag tggcacgacc agtcatcaag cccctgggag atgccaggac
     3241 acagttgata gtgaacatag aacccgagcg gagtgacata cgggagtatg aagtgatttt
     3301 taaccaaagc ctttgatgcc atgagtcagt acataccact tatcaggcgt caggcctcgc
     3361 gccttcgcct ttcgggtatc ggcgggaaca tacccaacct catccgggag gcagagcaaa
     3421 actctccgag ttatgatgag ttcacacata gtctgttcgc ttttgagata aaaggcagag
     3481 aagccaaaca gctgatgatg aggacgaagc ttgcaagact accattaaat catgatcttg
     3541 acagctatga ctttaatttt tcctccggac tatcggcctc acagctaaat caactccgtg
     3601 agctcaattg gctggatcag tgttacaata ttatgtttag cggaccatcg ggaacaggca
     3661 aaacatacat ctctgccggt ctgtgctacg atgccctgca gcgtggatac aaagcatact
     3721 tccgtggcat gaacgatata ctggccaccc tcaggctgaa ggaacttaca cctactgccg
     3781 gcaaggagtt taaaagactc tcagaagctc agcttattgt tattgatgat gttatgacac
     3841 tgcctcttgg aagggaagat ggcaacaggt tctttgtatt cttaaatcaa atctacgaga
     3901 caacttcttt cataatcaca accaacaaat caccggctga gtgggcaaga tcagtagatg
     3961 acgagaccct ggcaacagcg ctgcttgaca gattgcttta caagtgccag ttaatccaac
     4021 ttcagggcaa gagttacaga atgcaaaaca gaaagacaat tttttaaaca ttgatgaaaa
     4081 cttgcaagaa aataggcata cttttacacc agagtcactg cataattgtt cccgatttta
     4141 ctgtatcatt gttcccattt ttactgcact tcattttccc gaaatcactg tatttctttt
     4201 tcctgtttac actctttttt cattatatgg cttcctgatc ctgaaatctg agctgattat
     4261 cacgattatt tcagagaaac cgccatccgg ttcaggactt ttacaaaaat tcccggcagc
     4321 tgccgccagg cgcaggtttt tcgaattttt ctagccttta tcgtccggtg ttgctttgat
     4381 ctgtgtaaga tacagagaaa cagctgatta ataatttaat tatcacaatg attttcgaac
     4441 gctctttcag tatccggaaa tattttgttg taatctgaaa tataatttct actttaggca
     4501 actttaaatt catttgaaaa tttatataac aaatttaact ctatgttact tcatcagcag
     4561 tttgtccgga tggcaaagaa attctcatcc aaaattgcca taaaagacag gacgaccaac
     4621 cagtcactta ccttttcaaa ggcattgatc ggagctctga tcctgacttc aaagtttaag
     4681 aaatatgata agggatttat cggtattatg ataccgactt ccgcgggctg tgccctggca
     4741 tccgttgcag ctcttatgag cggcaggatc cctgttatga ttaattattc aacaggggct
     4801 gaagccaacg ccaggtatgc ccagaaaaaa tgcaggttta atacaatcat tgcctcaaaa
     4861 gccctgctgg acaagattgg ctgtccggta atcgagggaa tggttatgat tgaggatctg
     4921 atgaagggtg ttactactgc cgacaagctt aaggctgctc ttaaatcaat gctccccgta
     4981 aaattgctgc tgaacactat acataaaggg gctgaagatg attatgttgt catgcttttt
     5041 accagcggaa gcgaaaaaga cccgaaggct gttccgctta ctcacaggaa tatagtttca
     5101 aatatcgaga gcttcaccaa ggttatctcc ataacctcat cagatatcat cctttcaaac
     5161 ctggtcttct accatgtctt cggactgaca gtggatctgt gggttcctct ctatcttggc
     5221 atgaccatga ttacnnnnnn nnnnagtaaa ggtttaaatc aatagggtca acattggtga
     5281 ttttcagaca ataagcaata tagcccgaga ggaaaggccg acactgatgg ttggaactcc
     5341 cagctttttc tggggatatc ttcagaaatc cgagcccggt gattacaagt ctttcaggct
     5401 gatggtttcg ggtgccgaca aatgtccgga tgcattgcgg ggggaattca aaaagaaaca
     5461 tggtgttact ttgctggaag gttacggaac cacggaaact tctcctgtca tatcagtcaa
     5521 ttcccatgaa ttcaatcgtc ccggcagcac cggcagggtt attcccggcc ttcaggtaag
     5581 aattgagaat tttgaaaccg gggaggactg caagacgggt gaggttggaa aaataatggt
     5641 aaagggcgat tcagtgatga agggatattt tgaggatcct gaacagactg cagaggtatt
     5701 gaaggacgga tggtacaata ccggcgatat gggattcctc gatggagaag gctatttatg
     5761 gcatgccgga cgcttcaaga gatttacaaa gatcggcggg gaaatggttt cccttgtaaa
     5821 agtggagggg gttctcgaaa aactgcttcc ggaaggcgta aaatgttgtg ttgttgagat
     5881 ttccgatgaa aagaagggtt cctacattgt tgccgcggtg tctgccggga ttaacaagac
     5941 agaaatactc cggaagatga tgaaggaact gccgattatt tcactgccca ggcaatttgt
     6001 tgttatagag gaactgccga tgatgggaac cggaaaaata gatttccgca gggtaacgca
     6061 gatggtcggg gagatggagg aagtaaccgg cgacgccagg taggtacagc tgccggcagc
     6121 tataccgtta cggatcctat tcaaactcct tcggtactcc gcagattatc ctgaataagc
     6181 tgtcgggcga agtgttcctg tactggtgtt tttcgtccgg gctgacaagg gcgaaatcgc
     6241 ctgtcttcat aggatgttct tccccttccg gatcaacaag gacaccctgt ccttcaatga
     6301 cataattcat atgttcatag ggatggctgt gatatggagt atttccgccc ggttcgattg
     6361 tgaaaaccct gagggaatac agcggtgcac catccatgct tccgaggggc agctgtttcc
     6421 aggcaccgga agcgcctttc atttgtacct gcattttatc aacctggtca agagaaataa
     6481 ttttcatagc tgtttttttt atttgttctt atgcactgat gaaaaggctt ttgcttgaat
     6541 aaaccggatc acaggtaagg ttgaccctta gctttctcca tccggcctcg tcgcccggag
     6601 tggggatatg ggtgagatgc acctcacagt cacggaggag tttcaggttt tcaagtgcca
     6661 tttttgctgc cggatttgac aaagcactta ttccaagtac aatcagcgtt tcatcaacat
     6721 ccaggctgac cattttgcca tgaaggatgt ttttcttcag atgagtgacg gaatcgatta
     6781 tattttcagg taaaagatac attttatccg gcaggccggc gagatatttt gcggcattca
     6841 gcacaaggct tgatgaggcc tgcatcaggg gggagttttt ccccgtaatg atagtcccgt
     6901 cgtgtaattc tatggctgct cctgaaaaga ttccttcatt tccctttccg catttttcag
     6961 catccctggc agctttcctt gcgggctcaa caacatttct gtcctcttcc ctcaccccga
     7021 ccttgtccat tatcaggaca atacggtcca tggcctcctt ttccgacaac ccggcagcat
     7081 attcaaccga acatctgaaa aatcttctga ttatttcctg gcgggaagct tccctgatta
     7141 tactgtcctt aatgatgccc gcacttgccc tgttcactcc catgtcggtg ggcgactgat
     7201 acatggattc tccgcctgtg atcttttcaa tgatcttttt aagaagatgg aatgcttcca
     7261 ggtcacgggt ataattcaca ctttttatat tgtaggcctt catatggtaa tcatccagga
     7321 gcaccctgtc ttcctgatcg gcagttgcag cttcataggc gagattgacc ggatgatcgg
     7381 ccggaagatc ccacaccgga aatgtctcaa attttgcata gcctgccttg attcccttcc
     7441 tgtaatcatg gtaaaggttg cagagacagg ttgccagttt accgctgccc ggtcccggtc
     7501 cggtgacaat tacgatgggg cgggtggttt ttatctgttc attggctccg tatccgtcat
     7561 cgcttactat aagatcaaca tctgtggggt aaccttttgt gggataatgc agataaacct
     7621 ttacacccct gtgttgcagc ttgttcctga aaatcttcgc atgaggctga ttctggtacc
     7681 tggtgatgac aacagctgcg atatcaatgc cccattccct gaaatcgtca atggtcttga
     7741 gagcatcaac atcataagtg attccgaaat ccgccctgac tttttttctt tcaattgctc
     7801 cggcatgaat acaaagaatt acatcaatct tgtccttcag catctgcaga agcctcatct
     7861 ttacgttcgg atcaaaaccc ggaagaaccc tggcggcatg ataatcataa agtatcttgc
     7921 ctccgaactc cagatagagt ttctgatgga aacggtttac ccgctcaaga atggcagatg
     7981 tctgttcttc aagatattta tcattatcaa accctatctg atctaaaatc accattttaa
     8041 ttccctttaa aaatcattct tccccggtcc ggatcagaac ttcaatatta atgatttttt
     8101 cagttcacaa aataatcttt attccttatg aactcatttt tgatcattat atactgactt
     8161 aatgatatat ttgtatcagg gattaatttt ccggtaactc atattccgga ggcctgatcg
     8221 ggactaaatc atttgggtct gcaaggacag atgcggatgc catggaattg atgaggcgga
     8281 gtaaagtaaa aaaaaattga attttttaat cgcaaattat gaagtgttac aaattgttgc
     8341 tgacaatact tccggcactt ctcatactat ccggttgtag tgatacggac agttctaccg
     8401 ctaaaagaaa gaaattaaca aagaaggaga tcaattacat tgttgagttc gataaacctg
     8461 ttccgtcaat accttcaggt ttgaggatct cgacatttga cacagatgca actcctcctc
     8521 cgggaacatg gctggcttat gattcattgt tgaatacagg ggatctgggt ttaagagcaa
     8581 aaggcctggt tatcctgggc gcaggagaac ctattgttat ttgttgtgtg gattggctgg
     8641 ggatctccaa tgccgggtat gattcattcc gccgttctct gggcaatgca gccggaacaa
     8701 gcccggaaag ggtatcaata cacaccgttc accagcatga tgctccaaac tgtgatttca
     8761 ccgttgaaaa aatacttctg gatgcaggcg ttgatccggc atgctttgaa ggtacttttc
     8821 accgggaatt tatccgtaaa ctgtcgtatg tggtcaggaa ttcgctggaa aaatcacagc
     8881 ctgtaactca tatagggacc ggcaggggaa ttgttgatcg ggtggcttcc aacagaagga
     8941 tattgggaga aaacgggaaa gttatcggga caaggtggac cactacgaag gatccggctc
     9001 tgagggcact gccagaagga ctgatcgatc ccgaggtctc ggttgtgagt ctttggaata
     9061 atgatgatcc cctggcagtc ctgagctttt atgcttcaca tccacagagt tattaccgga
     9121 caggtatagc taatccggat tttccgggca tagcacgttt tttccgtcag cttgaagtac
     9181 cggatgccct tcatattcat tttaatggag ccggtggtaa tattggcgcc gggaaataca
     9241 acgacggagc tcatgaaaac cgtcttatac ttgcccagcg cctggctgaa ggcatgaaac
     9301 tggcatggga atcaacagta aaggaagaaa tcacacccga atccgtgaga tggaattata
     9361 tccccgtttc acttccccct gcgaaacatt tgcttgaact tgaaaaacgg atgacagaaa
     9421 tttcagatac cctgtattct cattccctgg cctccaagct tgcctggctg gagcgtacca
     9481 gggccggaat taaaactgac gtttcttgtc tttcagcaga aaataccaga atactctttt
     9541 tacccggtga attatttgtt gaatatcagc ttacagccag gtccatgaga aaggatctct
     9601 ttgtagccat ggcctcctac ggtgaatatg gaatgggata cataggtact gccgctgcct
     9661 atgatgaggg cggatatgaa acaggagacg aatcctttgt tgactccggc tcggaaacaa
     9721 tacttatgga cgcaataagc cggctgttgg gtaacaggta atatcagatg tctgtatcat
     9781 tgataatgaa tactcagatt gtgttgagct tatctctgtt gacatagacc ttcattatgg
     9841 catttatcag gtcatccata tctgccagag ttccgagcag tggacctgat gcccataaca
     9901 ttaccatatc ctcacaaacc tggtcacatt taggcagatg cagttcggaa ttgaatttcc
     9961 tgagcctggc ggcaccatac atttttctgt aaaccggcag tgtctgaata tgctgtgtcc
    10021 atggttccct gtggaggcca ttcgtgatat aagggctcag gcttatacct tcagcggcaa
    10081 ttgctttaag gaatttctca cggggagctc cgttgaacat atccttttta tacgaccacg
    10141 gataaagata ccacgaacct gcagtagtac cgggataaag tttctgcggg acaagccccg
    10201 ggcagtcctt aagatgttcg gtcaggtatt ttgcattcct gttgcggata taatgcctgt
    10261 ccataactcc cggtaactga ccgagcaggc aggcagcttc aaattcgttc attcggtatt
    10321 ttgatccgat agtttcggtc ctgcctcttt ttgaagtacc atgattatgt actgtgtagc
    10381 atttatccag aagctcttca ttgttactca caatgcctcc tccttcgccg catgaaagta
    10441 ctttgctggt ttggaagctg aaacaaccga catcacctat caggcctgct ttctttccct
    10501 ggtattctgc aaagtgagcc tgagctgaat cttcaataac cagcagttta tgctttttcg
    10561 caatttccat tatccgttcc atgttacagg gctgacccat catatgaaca ggcatcaggg
    10621 cttttgtccg gcttgttatt cttctttcaa catcatcagg gtcgagctgg aaggaatccg
    10681 gatcaaggtc tgccaatacc ggaagagctc tgctgaccag gattgatgct attgttccgg
    10741 gatcggtata gggcgaagtg atcacctcgt caccgggacc aatgccaaga gcttcaatac
    10801 atgtgttcag agattgtgtg cctgaaccgg tggcgatgct tgttttaatc cccatcagcc
    10861 tcgcaaattc attctcaaat ttcagaacct tattgtcggc tgactgaatc cggcaccata
    10921 ttccgctttt tgcagtatcc atcatttctg caatgacttt ttcgtcaata taaggccatt
    10981 cgggccatac ttttgccggt gctgcaggag tgcctcccag aatagccagt ttaccggaat
    11041 tgtcatttat aatttcaata tccttaaacg gggatatggc ccctgatgca acagcagcag
    11101 atcctgcaga tgctgttgta ataaatttcc gtctcgtaat ttttttcatt ttgtatatgt
    11161 tataaactta taaatcccca tttttataat cgatatggat agtatcaaag ttcgtcagtc
    11221 tatttaatat ttcaaaacat gaataacaga tttatcggtt ccggcattaa gattactgcc
    11281 attgccatat caatgtcaat gttgctgatc agttgtaata cctggccggg atttacagga
    11341 aataaaactg aaaagatcaa caggcttacc agggctgaaa agaaacaggg atttatgctg
    11401 ttgtttgacg gaaagacttt caacggctgg cgtggtctgg gaagggatca tgtccctgcc
    11461 ggcctctggg ttattgaaga cggaatgatc aaaaaagtgg atacaagaaa taaggagaaa
    11521 ctccctgatg gaaggcccgt tgaaggagga gatctgatga cggttgagac ttatgaaaat
    11581 ttcgaactga cttttgagtg gaagctgaat aaggcgggaa acagcggtgt gaaatacaac
    11641 gtgtcggaag agatgtcaca ggaatatgga tcaggatttt cggcactggg ttttgaatat
    11701 cagttgctgg atgatgatga tgaatcctac aggggtgtat taaaaccggc acagttcacc
    11761 ggctccctct atgatctgat accctcgcgg aatgttgctc tcagacctgt cggggagttc
    11821 aacagcagca ggataatagt tgacggtgat catgccgagc actggctgaa cgggatcagg
    11881 gtgcttgaat acgatttcgg gtcacaggaa ctggaagagg catacaggga cagtaaattc
    11941 agcaaaatac cggattttat caggaaacgg aaggcacata tcgttttaca gaaccataat
    12001 gatgaatcgt ggttccggaa tatcaagata aggataattg attgattgcc ggcagctggt
    12061 tttctgcttc agatgaatac cagggcatcc tttattaccc tgctgttgtt ggtgccgtaa
    12121 agttttatcc cgacacaggg aatggcatcc tggagtatgt cctgaacagg gatcagtgca
    12181 tccctgatat catctatatc gttcatgtca tcatactctt ccagaggcat ataaagatga
    12241 atatacagaa tgtactccgg gtcgtttatt tttttgagta ttaccagtat tctggaaagc
    12301 cacatcattg atgcggtatt gaaatactca atatccagcc tcaggttggt gattggtttg
    12361 gcttttaacg catactcact tacccagttc aggaccggct cataaacctt tgccgcattt
    12421 tcaggaattg attttccgga aaacaccagc tcgccggaat ccttattcag gtcaatcctg
    12481 ggagtttttg cagttttttc aagagtgaaa ttattaagtg acattttttt aaaaaaatat
    12541 ggcagaagaa aactaggcaa tacagtttca aaaatacttg atatggtgct aaatggaaaa
    12601 atttttatca aatactattg aacaatagtc aataaaatcg gcattttatc aacaaatttc
    12661 cctttcgggc caattgcggg atcaattccc cggtatggct tgcagactga atccggaatg
    12721 aattccatct gtctgacagc ttctttaaca aatttttaac tttcatcttt tttatttttc
    12781 cctgttttga aataatttca gccgttattt taaagcaacc cgcaagaaaa acagatattt
    12841 tatggatatt gtgattgaaa atctcaccaa gcgatacggt gtccagaaag ccgttgacaa
    12901 tatttcgctc agggtggcaa caggtgagat tctgggtttc ctcggaccca acggagcagg
    12961 aaagaccacc acaatgaaaa tcattaccaa ttttataact gctgatgagg gagaggttta
    13021 tatcggcggc agatcgctca gggatgatcc gcttgaaata aagaggcata tcggctatct
    13081 tccggagaat aatcccctct atcaggaaat gccggtaatt gattacctgg aattctgcgc
    13141 actgataaac aatattgaaa agcacagggt ggcgggaaga gttgccgaga tgatccgtat
    13201 taccggcctg aacagcgaga aacataagaa gattgccgaa ttgtcgaaag gatacagaca
    13261 gagagtcggt ctggcccagg ctatgatcca tgatccggaa atactcattc tggacgagcc
    13321 cacatccgga ctggatccca accagatagc cgagatacgc aagctgatac gcgagctggg
    13381 aagggaaaag acagtaattc tcagtaccca tatcctgccg gaagtggagg ctacctgcga
    13441 caggattttc attataaaca aggggaggct tgttgctgac ggtactgccg agagtctgag
    13501 gaaaaaggca cagggttccg acatattgcg cgtgagaatt gaggatggcg atatagatca
    13561 gattttcaag gcactgcaat ccttaaaggc agtgtcaatg gttgattttg ccgacaggca
    13621 gaaaaacagg tttgatgttc attgcaggag cgaggaggtg aaacgtgata ttttcaggct
    13681 ctgcgttgaa cggaactggg tacttaccga aataacgcca tttgaaacca ggcttgagga
    13741 tattttccgg gaactgacaa tgaattaatt ctgaataagc atgaaaacaa tctggataat
    13801 agcaaaacgg gaagtgaatt catattttga ttcgcttacg gcagttataa tgatagtgtt
    13861 attcctgggc ttcagcggat tctttacctg gatatcgggt tcggacattt tttttctcgg
    13921 gcaggcgagc ctgagatcct ttttcggaat tgcctactgg acactcttct tttttatacc
    13981 ggctctcacc atgaagactt ttgccgaaga aaacaagagc ggcacaattg aaatgctcct
    14041 gacaaaacct gtaaccgacc gccaggtgat ccttggcaaa tattttgcaa cactgatact
    14101 catagcaata gcccttgctc tcacacttcc ctatgtgatc acggtttcaa aaatagggaa
    14161 cctggatgct ggcggaacct tctgcggata tcttgccctt ctgctgatca gtgcggcata
    14221 tgcaagcatc ggactgttta caagcagtat tacatcaaac cagatagtgg catttctttc
    14281 tgcacttttt atcggacttt ttttccatat aatctttcag atcattgccg gaggaatgaa
    14341 aggattcctg ggacagattg taaactatct cagcattacc ttccattttg aaagccttgc
    14401 ccggggagtc cttgattcaa aggatgttat ctattttgca tcaataattt tcatgggatt
    14461 attccttacg gaagtttccc tttcaaaaag aaatgccttt aactaatcag actgatcatg
    14521 gtccgttgat ccgtcaataa ttcagaaaac gattatgagg aaagcaaaaa taacatcaac
    14581 aattatcatc gtcacaatga ttgtggtcgt tataaatatt ctttcaaaaa gctacaattt
    14641 caggctcgat tttaccgagg gtaaggaata cacactcagc aaggcaacca gggatattct
    14701 tgaaggtctt gacaaacctg tcacggtcac ggcatatttt tctaaagatc tgccgccgca
    14761 tatcggcagc atatctgatc acctcaggga tatgctgatt gaatatggca acaggtcaaa
    14821 gggtatggtg gtatttaaat tcgtcaatcc caatgaacag gaggatctgg aacaggaggc
    14881 gatgaaaaat ggtattcagc cggtgatgat aaatgtgagg gaaaaagacc aggtgaagca
    14941 gcagaaagcc tatctgggag ctgttgtttc ggttggcgac gacagggaga ccataccttt
    15001 tttccagccg ggtgcagcca tggagtatgc ccttaccacc tcaatcaaaa aactatccat
    15061 aactgacaag ccggcaatag ccctgatcca ggggcacggg gaaccgtcat taaatgaaat
    15121 gccccaggtt tatgcggaat taagcgtatt atacaatgtt gagcctttta ccatga
//
LOCUS       DAOSPX010000037        15165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4015_length_15165_cov_4.931701, whole genome shotgun sequence.
ACCESSION   DAOSPX010000037 DAOSPX010000000
VERSION     DAOSPX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4015_length_15165_cov_4.931701"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLZ75_03265"
     CDS             complement(<1..482)
                     /locus_tag="PLZ75_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HOS71452.1"
                     /translation="MKKKISGSLMVLFSRVLFRLVLSLAIIGFLQSCSTEKGGGILKL
                     RNDGRIHHIVVDDNSDQVVRFAAMQLKEYLERISGNEFRVSGRSDHKSKKNTIQLNIK
                     KDKALKWDGFMIKKTSKNIQLRSAESRGLLYAVYCLLEEAGCSFVYPGKEEEIIPQNE
                     "
     gene            complement(561..1658)
                     /locus_tag="PLZ75_03270"
     CDS             complement(561..1658)
                     /locus_tag="PLZ75_03270"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOS71453.1"
                     /translation="MSANNVKQIENRIFSIDFFRGFTMFMLVGGVWELFGNPDPDKSS
                     TLISFLNYQMDHAPWDGLRFWDLIHPFFIFIVGVAMPFSLTRRWERGDSWKKTLYHVL
                     QRCLFLLLIGWAINSGPNSSDFNNIMAQLSVSYLIAFLVMRKSVKWQLIFSFGLLLVA
                     HLLYIFWPVEGFNQPWVAGHNFGSWFDRLLTGEQYMNHDQWAPFNAIPSTANIIWGVL
                     TGRMLMTDWSQKKKLIILLLTGLAALLTGYLMSPYIPLVKRIWTVSYAFASGGWCLIG
                     MGISYWLVDILKLRKIPLFFAAFGMNPLLMYLLAGRLRSFFASLVNPFTYRIFAWGGQ
                     PVITMVSFLLISAMLWYVCYFLYRNRIFIRL"
     gene            complement(1771..3693)
                     /locus_tag="PLZ75_03275"
     CDS             complement(1771..3693)
                     /locus_tag="PLZ75_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71454.1"
                     /translation="MKLKEFTLPCVVLLLSLLHFSSCTSRYSPSEMDGEDWLRPFRVL
                     VVTGDQWDDPASYVVGKINTGTTEILHPGARKESDFYHIIILLKSWGIPFDIIRLDQQ
                     LLDRYMFLDMNGKPEYGTIIWNVNQTDNLMPQDYSILPEMTEQYGIGLIVLSDHIFRE
                     EILNTLGLKYTGIREGDTLLKVKGTHFITEGLLSPFRADTGTLVSMMIQRVRLSETDM
                     ERQHVAVSDDVITIVEQGSFPQVTVREYPSGGRAVWIGNDNNYIFYFQDMRTLLRRAI
                     TWTIGYNLYKTWDNDLIMIMDDPGMADNAWLDTWHFPTLSEDTIIQYLINPLLQHNAV
                     LNINFTPGFVNDAKERLEPTWTRVFTDEFGTKQNYVSSKRGYQKGVDLGVFTVMSHGL
                     THMQPDLASEPRWYGSPLSGEKAVNGWYQEFRDNRRNQEIPAAEQLWRMKTGMKWLKE
                     QFGVTPLEVVAGGNGVSTTYFNNTIKLAGQAGFGWYGWSGGYLGSDLAVVDWKFFGTP
                     DAPLIVGSPPNNHDYGIALEPEKFASIFDQYPDGRFISINEYIGYMHACNSGNLDRGK
                     KRLDITLDYDPHYCQFFEKHNSVWNLEFSDWLLEESGTVSSVTVDGKKAGKFTGKVEI
                     PRGCGSHRIDIHFRTK"
     gene            4048..4842
                     /locus_tag="PLZ75_03280"
     CDS             4048..4842
                     /locus_tag="PLZ75_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOS71455.1"
                     /translation="MFSGNIPARYIYTALTVPAFGIMYLFTALVIFAGFPFLVLRRKT
                     VIKMFMQFWAKSIFYIIGKRIHIQGTENLKTTKHYILLANHSSLFDIVAIASVFPDLT
                     WFGHERLLKIPVFRKVLQLTDYIPMRKATIANTKEMIRQLTARSATGNIAIFPEGTRT
                     LNGRLNDFFRGFIILLRTTEVNALPVTLNGFYELKPKNRFHIDFSSTLGMVIHKPVPG
                     EDLKNKSDREIAGHIKSIIESSLTGYHEGSTDEIQLKPGTLGNERR"
     gene            4829..5764
                     /locus_tag="PLZ75_03285"
     CDS             4829..5764
                     /locus_tag="PLZ75_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOS71456.1"
                     /translation="MKEDKWVFIINPIAGNGFAAEMESVIKAEVEKRGINGEIVFTEK
                     HGHAPEISERYASAGYRYIIGVGGDGTFNEIAAPLLHKKNVVAGIIPAGSGNDFIQIT
                     GFPSRFRAENWNSFFRAETIAIDAGFCNGKYFFNGLGLGFDANVASKNYTAEGELQRG
                     GKYKYIWHILGTILFYREKRMITVNNDSRNEDDCFINTVSNGRRYAGGFLLTPEAIAN
                     DGLLDVCNIKRLGLLQRLKILSMVPKGRHLGDKRVNYYRTTQLELEFGQKVPYHLDGE
                     VFFSERFDIRIIPGALNIIYNPSGRHFLKIPPEEI"
     gene            5825..6448
                     /locus_tag="PLZ75_03290"
     CDS             5825..6448
                     /locus_tag="PLZ75_03290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS71457.1"
                     /translation="MSSVFKTIRKGKLRLLYPFIVVLVLIAACGGRQDGKEKIVIRTE
                     LGDIHVSIDLRKAPVTSANFLKYVDAHLYDSACFYRLVQPGNQPADSVRIEVIQGGRY
                     KRAAERGFPPIAHETTVTTGILHRDGTVSMARSQPGTATSEFFICVGDQPELDFGGKR
                     NPDGQGFAAFGEVTSGMDVVRKIHSIDIQGQYLEKPVIITEIIREGR"
     gene            complement(6498..9338)
                     /locus_tag="PLZ75_03295"
     CDS             complement(6498..9338)
                     /locus_tag="PLZ75_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS71458.1"
                     /translation="MKKLIILLFIVAGCSVTEQHVSDVLSLAGEWEFRIDSLDQGLSR
                     KWYNEKFDESVKLPGSMAENGKGNEVTVFTDWTGDIIDRSYFTDARYEKYRQPGNIKI
                     PFWLKPVKYYKGAAWYQKEFDLPVTREDRRVILFLERCHWESKVFVNGREAGTQNSLA
                     APHEYDITDLVSTGKNKISIRIDNRIIIPVGVNSHSISDHTQSNWNGITGDIYLQFRP
                     QVYIDNVRIVPDIKENNAKVIVSIRNPRKTGFKGTLRLRAESFNTRQKQILPEKSEKI
                     STDTDEQQVIISYKIRKPLLWSEFSPSMYRMSVTLTESDKGLSDNCLVDFGMREFTTS
                     GTRFEVNGRPVFLRGTTECCIFPLTGYPPSDEASWEKVLQTCRDYGLNHMRFHSYCPP
                     EAAFRAADKLGIYFHVECSSWANQGSSIGDGGIIDRFIYEEGDRIIKEYGNHPSFCML
                     AYGNEPAGKNQNAFLGQLINYWKSNDGRRLYTSAAGWPVIPENDYHLIPAPRIQQWGE
                     GLKSIINREAPQTLFDFREIIGKYEVPVVGHEIGQWCVYPDFSEINSYTGVLKPANFE
                     IFRETLEKNNMGDQAEDFLKASGKLQVLCYKADIEAALRTPGYGGFELLQIYDFPGQG
                     TALVGILNPFFGSKGYVSPEEFRMFCNETVPLARLEKMIYKAGEKLTADIEIAHFGEK
                     PLKNARILCRLVSADGKIFGEETMHVDEIEIGNGKTIGTFKTDIPDFARAEQLDFEIS
                     IDGTSFRNHWNLWAYPSSVKSDPREVYVTDMIDGKTEDILAGGGSVLLLTYGKVGRTQ
                     GAQVAIGFSSIFWNTAWTKNQPPHTLGILCDPGHPLFTGFPTEYHSNWQWWDPVTHSQ
                     AMILDAFPAKLKPLIQPIDTWFENRKLALAFEVKAGGGKMLVCSIDLKDIPEERPVSK
                     QLIASILNYMNSDSFNPQTDIELKQVYELINK"
     gene            complement(9474..10868)
                     /locus_tag="PLZ75_03300"
     CDS             complement(9474..10868)
                     /locus_tag="PLZ75_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOS71459.1"
                     /translation="MKGQKYNWHYLLGISLVSAMGGLLFGYDWVVIGGAKPFYEPFFG
                     ITGSPGLQGWAMSCALTGCLAGAVLSGWLSDRFGRKKLLILSAFFFVAASIGTGSAGT
                     FTAFVLYRILGGIGIGLTSNLSPMYIAEVTPGNIRGGFVSINQLTIVIGILAAQLINW
                     RIAQPVPPGASTVYILESWNGQTGWRWMFYACTIPALLFFILMWFVPESPRWLAKNKA
                     NHLKVKKILTRIGGAEYAETELVSIDETLRNTPGKTDFRVLRQPGISKLLVLGIGLAV
                     FQQWCGINVIFNYAQEIFSNAGYSVSDILFNIVITGSVNLIFTFVGMFTVDRLGRKAL
                     MLIGAGGLAVIYAVIGSMYFFHLQGMLLLIMVVIAIGCYAMSLAPVTWVILSEIFPNR
                     IRGFAMSVATFSLWTACFVLTYTFPLLNHALKVSGTFWLYGFTCVLGFWFIRKRLPET
                     KGKTLEEIEHELIR"
     gene            complement(11448..13175)
                     /locus_tag="PLZ75_03305"
     CDS             complement(11448..13175)
                     /locus_tag="PLZ75_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOS71460.1"
                     /translation="MNLSTVDLIIVIVFLVGITLFGIFQMKKKRDTSDGFFLAGRNLR
                     WPIIGLSMFAANISTIHVVGLASSGFNEGMVWGNFEWLAGVTLIFLALIFAPFYFKSR
                     IQTLPEFLERRYDARSRTVFAFIAILGALFVHIGLSLYTGALIFQRFIGISIWTSIAV
                     LSGLTLIYYLAGGLRAVVYTQAVQAGVLIFGSVALTVAGLMKLGVLMDESGMIGSFID
                     NFMNLARLGQMDVLHTKASIAGLTGEINKEFSANGFDSVSGSGLTWYAAFLGYPILGL
                     WYWCSDQTIVQQVLGAKTKDDAQKGPIFAAFIKLFTPFIMILPGIIGFVLLKQEIFDA
                     AQSAVPAVTKPGDMTLTVLIDQLLPVGLQGIFAAGLMAALMSTIAAALNSISTLVSID
                     IYKRIKPETSDKNLIKIGRIAATFIMMIAAMWSTQGDKFSSVFEAINKVAAVLSPPIA
                     AVLLLGVLYKKGTKEASLITLTTGLIMGITVFVLDFKFTSSGTSIVTESWGVPFMMQA
                     WWLFCICVAIYLVASIFTPKPDPLVIEKYTWDNPLSIMKGKILNINDVRVLILILILA
                     LIVLYKVFS"
     gene            complement(13421..13549)
                     /locus_tag="PLZ75_03310"
     CDS             complement(13421..13549)
                     /locus_tag="PLZ75_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71461.1"
                     /translation="MEMHKYTGKDIENWMDRELIIETSDVKNIKSTCAGGSSIAGF"
     gene            complement(13599..13898)
                     /locus_tag="PLZ75_03315"
     CDS             complement(13599..13898)
                     /locus_tag="PLZ75_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71462.1"
                     /translation="MELVKIFKKIKDFGVTASLDMAYTEPSSESGKADWKKILKSVLP
                     YVDIYIPSIEETLYMLMRDYLNDINTNDNLINNLDVNVLPRLEDALLFWSESGRN"
     gene            complement(13935..14354)
                     /locus_tag="PLZ75_03320"
     CDS             complement(13935..14354)
                     /locus_tag="PLZ75_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOS71463.1"
                     /translation="MVAGHICLDITPVFRTDNYLDIQQVFTPGHLTNMMGVKIAAGGV
                     VPNVGISLSIMGLNCQLIGKIGKDFFGEDILNFLKQLNVSESIVRTTSENTSYTVVIA
                     PPGVDRIFFQDSGANNKFTYDDINFEIVKKARLFLFG"
     gene            complement(14411..15097)
                     /locus_tag="PLZ75_03325"
     CDS             complement(14411..15097)
                     /locus_tag="PLZ75_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOS71464.1"
                     /translation="MKRSEINEAIKISKDLLERNNFRLPAFAYWTINEWEQNKDKIAT
                     IVRVMQGWDITDFGSGRFKEVGAVLFTIRNGDIKNNGIGTPYAEKIIILQHEKGQKIP
                     MHCHRIKTEDIINRGGGVMCIQLFGSNQEGKLDNYVNVEVFMDGIKYCFKAGETIDIQ
                     PGCSITLSTYIYHAFWAKKGAGDLIVGEVSSINDDKTDNFFVEQARRFSSIEEDEAIL
                     HPLVNDYDKL"
BASE COUNT         4328 a         3423 c         3022 g         4392 t
ORIGIN      
        1 tcctcattct gaggtatgat ctcttcctcc ttcccgggat atacaaagga acatccggct
       61 tcctccagaa ggcagtaaac cgcataaagc aatcccctgg attcagcaga ccggagttgg
      121 atgttcttac tggttttctt aatcatgaaa ccgtcccatt tcagggcctt gtcctttttg
      181 atattcagtt gtatggtgtt ctttttgctt ttatggtctg accgtcctga aacacggaat
      241 tcatttccgc ttattctctc caggtactct ttgagctgca ttgcggcaaa gcgtacaacc
      301 tggtctgaat tatcatccac cactatatga tgtatccttc cgtcattccg tagtttcagg
      361 attcctccgc ctttttcggt gctgcaggac tgaaggaatc cgatgatggc aagacttagc
      421 accaagcgga acagaacgcg ggaaaacaga accattaagc ttccggatat tttctttttc
      481 atagagttga tttataaaaa tattgtcctg aaatactcct gcacagatac tgtctcctgc
      541 atccgtacag cggatcgtct ttacagcctg atgaatatcc tgttcctgta caggaaataa
      601 cagacatacc acagcattgc ggatataagc aggaacgata ccattgttat taccggctgt
      661 ccgccccagg cgaaaatacg gtatgtgaac ggatttacaa ggctggcgaa aaatgaacgc
      721 agtctgccgg ccagcaggta cataagcaat ggattcattc cgaaggccgc aaaaaacagc
      781 ggtattttcc tcagtttcaa aatatcaacc agccagtagg aaattcccat tccgatcagg
      841 caccatcctc cgcttgcaaa ggcataggag actgtccata tgcgtttgac aaggggtatg
      901 tacgggctca ttaagtatcc tgtcagaaga gcggcaaggc cggtcagtaa aagaataata
      961 agttttttct tctgtgacca gtcagtcatg agcatcctgc cggttagaac tccccatatg
     1021 atgtttgccg ttgacggtat ggcattgaat ggtgcccact ggtcgtgatt catatactgt
     1081 tctccggtaa ggagcctgtc aaaccaggag ccgaaattgt gaccggcaac ccagggctgg
     1141 ttgaagcctt caaccggcca gaagatgtag agaagatggg caacaagaag caatccgaat
     1201 gaaaatatca attgccattt tacggatttc cgcattacca ggaatgcaat gagatagctt
     1261 accgatagct gtgccattat gttattgaag tccgaactgt ttggtcctga gtttattgcc
     1321 cagccgatca gcagcagaaa aagacagcgc tgcaaaacgt ggtacagggt ttttttccag
     1381 ctgtcacctc tttcccatct ccttgtaagg gagaagggca tggctactcc cactatgaaa
     1441 atgaaaaagg gatgtatcag gtcccagaaa cggaggccgt cccagggagc atgatccatc
     1501 tggtagttga gaaaggaaat taaagtgctg cttttatccg gatcaggatt gccgaataat
     1561 tcccatacac ctcccacaag catgaacatg gtaaaaccac ggaaaaaatc aattgaaaaa
     1621 atacgatttt ctatctgttt tacattgttt gcgctcatta tgctgatatt ttaaattggt
     1681 attgtccgcc ggtatagctg tatccatatc tgtctgaagt cctttcatcg gtttgttctg
     1741 taaaaaacaa ctgcagagtt tgagtaagga ttattttgta cggaagtgaa tatctatcct
     1801 gtgacttccg caaccccggg gtatttcaac tttcccggta aatttgcctg cttttttccc
     1861 gtccacggta acggaactga cagttccgct ttcttcaagc agccagtcgg aaaactccag
     1921 gttccagact gaattatgtt tttcaaaaaa ctggcagtaa tgggggtcat aatccagtgt
     1981 gatatcaagt ctttttttcc ccctgtccag gtttcctgaa ttgcatgcat gcatgtaacc
     2041 gatatattcg ttgatgctga tgaagcgtcc atccggatac tgatcaaata tggaagcaaa
     2101 tttttccggt tccagggcga taccgtaatc atggttattc ggaggagaac caacaatcag
     2161 gggggcatcg ggtgtcccga aaaatttcca atcgacaaca gccaggtcac ttccaagata
     2221 gcctccgctc catccgtacc atccaaatcc tgcctgacct gccagtttta ttgtattatt
     2281 gaaatatgta gtggagacac catttccgcc ggccaccact tcaagaggag taacaccaaa
     2341 ctgttccttc agccatttca tgccggtttt cattctccag agctgttcag ctgcaggaat
     2401 ttcctggttt ctcctgttgt ctctgaattc ctggtaccag ccgttcacgg ctttctcccc
     2461 gctgagcggc gagccgtacc agcgcggctc tgaagccagg tcaggctgca tatgtgtgag
     2521 tccgtgggac ataaccgtga aaacgcccag gtcaacacct ttctgataac cgcgtttgct
     2581 ggaaacataa ttctgttttg tcccgaactc atccgtaaaa acccgggtcc atgttggctc
     2641 cagcctttct ttggcatcgt tgacaaaacc cggggtgaaa ttgatattca gaacagcatt
     2701 gtgttgcagt aaagggttga tgaggtactg tatgatagta tcctcggaca gtgtcgggaa
     2761 gtgccaggtg tcaagccatg cgttatcagc cattcccgga tcgtccatga tcatgatcag
     2821 atcattatcc catgtcttgt aaagattgta tccgattgtc cacgtaatgg cccgtcgcag
     2881 caatgtccgc atgtcctgga aatagaagat ataattattg tcatttccta tccagaccgc
     2941 acggccgccg gaaggatatt cccttaccgt tacctgcgga aaggaaccct gttcaacaat
     3001 tgtaatcaca tcatccgaaa cagccacgtg ctgacgttcc atatcggttt ccgacagcct
     3061 gacgcgctga atcatcatgc ttaccagtgt gccggtatca gccctgaaag gagataagag
     3121 tccttcggtt atgaaatgtg tgccctttac tttcaacagg gtatcgcctt ccctgattcc
     3181 ggtatatttt aagcccagtg tattcagtat ctcttcccgg aaaatatgat ccgaaagaac
     3241 aataaggcca atcccgtatt gttccgtcat ttccggaaga atggaataat cctgcggcat
     3301 cagattatcc gtctgattaa cgttccagat aatggttccg tactccggtt tcccgttcat
     3361 gtcaagaaac atgtaccggt caagcaattg ctggtccaga cggatgatat caaagggaat
     3421 cccccaggat ttcagcaaaa tgataatatg ataaaaatca gactccttgc gagctccggg
     3481 atgtaaaatc tctgtagttc cggtatttat tttacccacc acatagcttg ccggatcatc
     3541 ccactgatca cctgtaacaa cgagtaccct gaacggcctg agccagtctt cgccgtccat
     3601 ttccgaagga ctgtaccggg aagtgcatga tgaaaaatgc agcagtgaaa gtaatagtac
     3661 aacacagggc agggtaaatt ctttcagttt cattataaag ctcaaattat ttaatgcacg
     3721 tatcctgtta ttattaaaat aatttaggcg gacacggtaa aatatatgat tccgtgatag
     3781 ttggcagaat tgatgaatga ttctgagttt attaccaggc actgttatcg gtttaataat
     3841 aaagatccct gaaaagtgct cttaagtatg agataatcag gcaatatact ttgattttca
     3901 ataaaaatct catgcaaatt actgttttta ttcagtaatc cggtcatata ccggcaaata
     3961 aatgtacttc atttcatcag attcttccgg tttttttaga attaatattt ttacttttat
     4021 gcggtttaat aatgaatatc cggagatatg ttctcaggaa atataccggc aagatatatt
     4081 tacaccgcac ttaccgttcc ggctttcggg attatgtatc tgtttaccgc cctcgttata
     4141 tttgccggct ttccgtttct tgttttaagg agaaaaacgg taataaaaat gttcatgcag
     4201 ttctgggcaa agagtatctt ttatatcatc gggaaaagaa tacatataca gggaacagag
     4261 aacctgaaaa caacgaaaca ctatattctc ctggcaaatc attccagtct tttcgatatt
     4321 gttgccattg cttccgtatt tcccgacctg acatggtttg gccatgaaag gcttttaaaa
     4381 attcctgttt tccggaaggt gcttcagctg acagattata ttccaatgcg gaaagcgaca
     4441 atagcaaaca caaaggaaat gatccggcag cttactgccc ggtccgcaac aggcaatatt
     4501 gccattttcc cggaggggac aagaacgctg aacggcaggc tgaacgattt tttccgggga
     4561 tttatcatcc ttctgaggac gacggaggtt aatgcccttc ccgtcactct gaacggattc
     4621 tatgaactca agcccaaaaa cagattccat attgatttca gttccacact ggggatggtg
     4681 attcataaac ctgtgcccgg tgaagatctg aaaaacaaaa gcgacaggga aattgccggg
     4741 catataaagt caattattga atcatcactg accggatatc atgaaggtag tacggacgaa
     4801 atacaactta agccgggtac attaggaaat gaaagaagat aaatgggttt ttataataaa
     4861 ccccatcgcg ggaaatggtt ttgccgcgga aatggaatcc gtgatcaaag cggaagtgga
     4921 aaaacggggt attaacggag aaattgtttt taccgaaaaa catggccatg ccccggaaat
     4981 atcggaaaga tacgcttccg ccggttacag gtatataata ggggtagggg gcgacggtac
     5041 cttcaacgag atagccgctc cgctcctgca taaaaagaat gttgttgcgg gaattatccc
     5101 ggcaggatcg gggaatgact tcattcagat aacaggcttt ccctccaggt tcagggcgga
     5161 aaactggaac agttttttca gggcggaaac gattgccatt gatgcaggtt tttgtaacgg
     5221 aaaatatttt tttaacggcc tggggctggg atttgatgca aatgttgcgt caaagaacta
     5281 tactgcagaa ggtgaattgc aacgcggggg caagtataag tatatctggc atattctggg
     5341 cactattctt ttttaccggg agaaaaggat gattacggta aacaatgaca gcaggaacga
     5401 ggacgactgc tttatcaata ctgtttccaa cggccgcagg tatgccggcg gtttccttct
     5461 caccccggaa gcaattgcaa acgacggtct gctggatgtg tgcaatataa aaagactggg
     5521 actccttcaa cgcctgaaaa tactttcaat ggttccgaaa ggcagacatc tcggcgataa
     5581 aagggtgaac tattacagga caacacagct ggaacttgaa ttcggacaga aagttcccta
     5641 tcatcttgac ggcgaggtct ttttttctga aagatttgat atcaggatca tacccggggc
     5701 actgaacata atttacaatc cgtcgggcag acattttctg aaaatacccc ctgaagagat
     5761 ttagatgttt tctgtgtgcc gggatcagga aattatacat gttttaatct ttaataatca
     5821 gaccatgtca tcagttttta aaaccatccg gaagggaaaa ctccggctgt tatacccgtt
     5881 tattgtcgtg ctggtgctga ttgccgcctg tggcggcagg caggacggaa aggaaaaaat
     5941 agtgatcagg acggaacttg gcgacataca tgtaagcatt gacctgcgta aggccccggt
     6001 cacatctgcc aatttcctga aatatgttga tgcgcatctt tatgacagtg catgctttta
     6061 tcgtcttgta cagcccggca accagccggc ggactctgtc cgcatagagg tgattcaggg
     6121 aggcaggtac aaaagggcgg ctgaaagagg atttcctccc atagcacatg aaacgactgt
     6181 cactacgggg atcctgcatc gcgacggtac ggtttccatg gcacgcagcc agcctggaac
     6241 ggcaacatcg gaatttttta tctgtgtggg ggatcagccc gagcttgatt ttggagggaa
     6301 acgcaatccc gacggacagg gttttgcagc cttcggcgag gttacctcag gaatggatgt
     6361 tgtgagaaaa atccattcga ttgatataca gggacaatat cttgaaaaac cggtgataat
     6421 aaccgaaatc atcagggaag gacgataagg cttaaatccg gctctcatct gtcagcttgc
     6481 cgcgatagta atcactttta tttgtttatc agttcataaa cctgtttcag ttcaatatct
     6541 gtctgagggt taaaagaatc gctgttcata taattaagga tagaagcgat cagttgttta
     6601 gaaaccggcc tttcttcggg tatatctttc agatctatgc tgcaaaccag catttttccg
     6661 cctccggctt ttacttcaaa ggccagcgcc agttttctgt tctcaaacca ggtatctatc
     6721 ggctggatga gaggttttaa ctttgccgga aatgcatcca gtatcattgc ctgggaatgt
     6781 gtgacaggat cccaccattg ccagttgctg tgatattccg tcgggaaacc tgtgaacagg
     6841 ggatgcccgg ggtcgcacag tattcccagg gtgtggggag gctggttctt agtccaggca
     6901 gtgttccaga aaatacttga aaaaccaatt gcaacctgtg ccccttgtgt tctgccgact
     6961 tttccatagg ttaaaagcag cacggaacca cctcctgcaa ggatatcctc agttttcccg
     7021 tcaatcatgt cagtcacata aacttcccgg ggatctgact ttactgatga cggataagcc
     7081 cataaattcc agtgattcct gaaagatgta ccgtcaatgc tgatttcaaa gtcgagttgt
     7141 tcagcccggg caaaatcagg aatatccgtt ttaaatgtac cgatagtttt tccattacct
     7201 atttcaatct catcaacatg catggtttcc tcaccaaata ttttcccgtc cgctgagact
     7261 aatctgcaca ggatccgtgc attttttaac ggtttttccc cgaaatgggc aatttcaata
     7321 tctgccgtga gtttttctcc ggctttataa atcatctttt caagccttgc caggggaacg
     7381 gtttcattgc agaacattct gaattcttcc gggcttacat atccctttga tccgaagaaa
     7441 ggattaagaa tcccgaccag tgctgttccc tgtccgggga aatcataaat ctgcagaagt
     7501 tcaaaccctc cgtagccagg tgtcctcaat gctgcttcta tatcagcttt atagcaaagg
     7561 acctgaagtt ttcctgaagc tttaaggaaa tcttcagcct gatcacccat gttattcttt
     7621 tcaagtgtct ccctgaaaat ctcaaaattg gcaggtttta agactcctgt ataggaatta
     7681 atttcactga aatcgggata aacacaccac tgtcctatct catggccgac aacaggtact
     7741 tcatatttcc cgatgatttc ccggaaatcg aaaagtgtct gaggagcttc cctgttgata
     7801 atgcttttaa gaccctctcc ccattgctga atcctgggag cagggatcaa gtggtaatca
     7861 ttttcaggaa taacaggcca gccggccgca gatgtataca atcttctgcc gtcatttgat
     7921 ttccaataat ttattagttg tcccagaaat gcattctgat tttttcctgc cggttcgttt
     7981 ccataggcca gcatacagaa tgacggatga tttccatact cctttattat tctgtcacct
     8041 tcctcatata tgaacctgtc aattattcct ccgtccccga ttgaacttcc ctggttcgcc
     8101 catgaactgc attcaacatg aaaatagata cccagcttgt ctgcagctct gaaagctgct
     8161 tcgggagggc agtaggaatg aaatctcata tggttgagtc cgtagtcacg gcaggtctga
     8221 aggactttct cccatgatgc ctcatcagaa ggaggataac cggtcagcgg aaaaatgcag
     8281 cattcggttg tgcctctgag gaaaaccggc cgcccgttga cttcaaatct ggtaccggaa
     8341 gtggtgaatt cccgcatccc gaaatcaacc agacaattat ctgacaaacc cttgtctgat
     8401 tctgtcaggg tcactgacat cctgtacatt gaaggtgaaa attcgctcca cagaaggggt
     8461 ttgcggatct tataacttat aataacctgt tgttcatctg tatcagtact gatcttttca
     8521 cttttttccg ggaggatctg tttctgcctt gtattgaaac tttctgccct gagcctcaat
     8581 gtacctttaa aaccggtctt ccgcggattc ctaatgctta caatgacttt cgcattattc
     8641 tcctttatgt ccggcactat cctgacatta tcaatataga cctgtggccg gaattgaagg
     8701 taaatgtcac cggttatgcc gttccagttg gattgtgtat gatcacttat gctgtgcgaa
     8761 ttgaccccca cgggaattat gatccggtta tcaatccgga tactgatttt gtttttccct
     8821 gtggaaacca gatcggtaat atcatattcg tgtggtgcgg caagactgtt ttgtgttccg
     8881 gcctcccgtc cattgacaaa tacctttgat tcccagtgac atctttcaag aaaaagtatc
     8941 acacgcctgt cttcccgggt caccggcaag tcaaattcct tctgatacca tgcagctcct
     9001 ttataatact tcacaggttt aagccagaat ggtattttaa tattcccggg ctggcggtat
     9061 ttttcatatc tggcatccgt aaaatacgac cggtcaataa tatcacctgt ccagtcagta
     9121 aatactgtaa cttcattccc tttaccgttc tcagccatcg agcccggcag cttcaccgac
     9181 tcatcaaatt tctcattgta ccatttccgg gataaaccct ggtcgagtga atcaatccgg
     9241 aattcccatt ctcccgcaag ggaaaggaca tcactgacat gctgttcggt aactgaacaa
     9301 cctgcgacta taaacagcag aatgattaat ttcttcatat atcaatgatt ttgattttaa
     9361 aggtaatatg aaccattaag gcttcgcggg aagtgccccg ctatgtttgt gcatttataa
     9421 ctacatgtct ttatcaggaa tatcccgtta atacaatttt cccggattcg gtctcaccgg
     9481 attagttcat gctcaatttc ttcaagtgtc tttccctttg tctcgggaag tcttttccgg
     9541 atgaaccaga atccgagaac acatgtgaat ccgtaaagcc agaatgttcc cgaaactttt
     9601 agtgcatggt tcagcagagg gaaagtatat gtcagtacga agcaggctgt ccagagggaa
     9661 aatgtggcaa ctgacattgc aaagccccgt atcctgttgg gaaatatttc tgacaggatc
     9721 acccaggtga ccggagcaag tgacatcgcg tagcagccta tggcgataac taccattatc
     9781 agtaaaagca taccctgaag atggaaaaag tacatgcttc ctatgaccgc ataaataacc
     9841 gcaagtcctc cggctcctat aagcataagt gcctttctgc cgagcctgtc aaccgtgaac
     9901 atcccaacaa aagtgaatat aagattaaca cttccggtaa tgacaatgtt gaataatata
     9961 tcagatacac tgtagcctgc atttgagaat atctcctgtg catagttgaa tataacattt
    10021 atcccgcacc attgctgaaa gactgcaagc ccgatgccca gtacgagcaa ttttgatatt
    10081 ccgggttgcc tcagaacgcg gaaatcagtc ttgccgggag tgtttctgag ggtttcatca
    10141 attgaaacaa gttcagtctc agcatattca gctcccccga tcctggtcag gatctttttc
    10201 actttcaggt gatttgcttt attcttggcc agccaccttg ggctttcagg gacgaaccac
    10261 ataagaatga aaaacaataa ggccggtatg gtacatgcat agaacatcca gcgccatccc
    10321 gtctgaccat tccatgattc caatatataa actgttgaag caccgggtgg aacaggttgc
    10381 gcgatcctcc agtttataag ttgtgctgcc agtatcccta tcacaattgt aagctggttt
    10441 atggacacaa aaccgccccg gatattcccc ggggtcactt cagcaatata cattggtgac
    10501 aggtttgaag tcagtccgat tcctatccct cccaggattc tgtaaagtac aaaagcggta
    10561 aatgtgccgg ctgatcctgt cccgattgat gcagcaacaa aaaagaatgc tgagagaatt
    10621 aataatttct tacgtccgaa cctgtcactc agccagcctg ataagacggc accagccaga
    10681 cagcctgtca gggcacaact catggcccag ccctgcaaac ccggtgagcc ggtaatacca
    10741 aagaatggct cataaaatgg ttttgcccct ccgatcacca cccagtcata gccaaaaaga
    10801 agtccgccca tagccgatac cagggagatg ccaagcagat aatgccagtt atacttttgt
    10861 cctttcactg caattgactt aatgaactaa atccggacaa tctgaccaca ttttgccgta
    10921 acaaactgac cacatagcag ccggtccttt aagaagagct ttctcacggt aaaaagcggg
    10981 tttacaaata tgtctatttt aattcattat ttccatacct ttttgccctt catttgtctt
    11041 actcaggaac tctcccttat catggaaagc tttccggaga aaaagaatgc cgtgttgtgc
    11101 cactaaaaaa gaaattgtca ggaagttaat gaatctaatg gtgaaggcat acagaggacc
    11161 gtggactccg gctttttgtt tgccgcgaac ttcccggcaa actaaaaagc aagattttta
    11221 aataatggga aattagtatt ggccacgcgt aatagctgat ctatctcaag aggatcggta
    11281 gagcagccaa gggtctttaa aactctgttt atacgacctg ctttttcgac gatttgaaca
    11341 cttatgctgc cgcttactca atataactta caggtaataa acattctaat aaatactgat
    11401 aaaatacttc tttcactaaa cggcttagac accagccact gaaataccta tgaaaaaact
    11461 ttgtacaaaa cgatcaaagc tagtataaga attaatatca gaacacgaac atcatttata
    11521 tttaaaattt tgcctttcat aattgataga gggttatccc aagtgtattt ttcaataacg
    11581 agaggatcag gttttggtgt aaatatgctg gcaactaaat aaatagctac acatatgcaa
    11641 aacagccacc atgcttgcat cataaaaggc actccccatg attcggttac aattgaagta
    11701 cctgacgaag taaatttaaa atcaagtaca aacactgtta ttcccataat taatcctgta
    11761 gttagtgtaa ttaaggatgc ttccttagtc ccctttttgt atagcactcc taacaacagt
    11821 acagctgcta taggaggcga taatactgct gctactttat ttatggcttc aaaaacactt
    11881 gaaaatttat ctccctgagt cgaccacatc gcagcgatca tcataatgaa tgtggcagca
    11941 attctaccaa tctttattaa gtttttatca gaggtttcgg gtttaatccg cttataaata
    12001 tcaattgata ctaatgtaga tatactattc aacgctgctg ctattgtact cattaaagca
    12061 gccatcaaac ccgccgcaaa aattccctgt aatccaactg gtaaaagctg atctattaaa
    12121 actgtcaaag tcatatcacc tggcttcgtt actgctggta cagccgattg agctgcatca
    12181 aaaatttctt gctttaataa tacaaatcct attatacctg gaagtatcat tataaaggga
    12241 gtaaaaagct ttatgaaagc ggcaaaaata ggtccctttt gggcatcatc ttttgtttta
    12301 gcacctaaca cttgttggac tatggtttgg tcagaacacc agtaccacaa tcctagtata
    12361 ggatatccaa gaaatgctgc ataccaggtc aacccactcc ccgaaacaga atcaaaacca
    12421 ttggctgaga attccttgtt tatttcacca gttaatcctg cgattgatgc cttggtatgt
    12481 aaaacatcca tctgtcctag tctggcgaga ttcatgaaat tatcaataaa actacctatc
    12541 ataccagatt catccattag gacgccaagc ttcattaatc ctgctactgt caatgcaaca
    12601 gaaccaaaaa ttaatacacc tgcctgaaca gcttgggtat aaactactgc ccttaaacct
    12661 cccgcgaggt aatatattaa ggtgagcccc gacaatacgg ctattgaagt ccagatgcta
    12721 attccaataa atcgctgaaa aattaatgca ccggtataaa ggcttaggcc aatatgtaca
    12781 aacaaagctc cgagtattgc tataaatgca aagacagtcc tcgatctggc atcgtacctg
    12841 cgttctagaa actcaggtaa ggtttgtatc ctgcttttaa aataaaaagg ggcaaaaata
    12901 agggcaagaa aaattaatgt caccccagct aaccattcaa aattccccca aaccattcct
    12961 tcgttaaaac ccgatgaagc aaggcctaca acatgaatag ttgaaatatt tgctgcgaac
    13021 attgataaac ctattatagg ccaccttaaa tttcttcccg caagaaaaaa gccgtcactt
    13081 gtatcacgtt ttttcttcat ttgaaatatt ccaaacaggg taatcccgac caggaatact
    13141 atgacaatta ttaaatcaac tgtgcttaaa ttcatatttt attaactatt aaacaaactt
    13201 tctatctttt ttccccttta atacaccaag ttgcttatca tatttccagt aagaatcttc
    13261 aatttcgtac cgaataacag gtatactttt tttaccattt cagaaattgt atcaaaatct
    13321 tttatattac aaaaacatca taaaccaaaa tactttgtat agctactgcg tggggaatct
    13381 taactgctcc ctcaataggt ctgatattta gaaatgcagc ttaaaatcct gcaatactgc
    13441 tacctccagc acatgttgat tttatatttt ttacatcaga cgtttcaata attaactctc
    13501 tgtccatcca attctcaatg tcttttccgg tatacttatg catttccatt aatgcttcct
    13561 ttagctgagt tttgatatag taacccttga taccacactt aattacgacc actttcgctc
    13621 caaaataata gagcatcctc aagccttggc aatacattaa catctaagtt gttaatcaga
    13681 ttatcattgg tgtttatgtc atttaggtaa tctctcatta acatataaag tgtttcttca
    13741 atactcggga tgtaaatatc tacataaggg agtacacttt tgagaatctt tttccagtca
    13801 gccttccctg attctgagga tggttcagta taagccatat ccagggaggc agttactcca
    13861 aaatccttta tctttttgaa gatttttacc aattccatgc catcattttc acatattttc
    13921 ctaattaaag gtggttaacc aaaaaggaat agcctagctt tttttacgat ttcaaaattg
    13981 atatcatcat aagtaaactt attatttgca ccagaatctt ggaagaatat cctgtcgaca
    14041 ccgggagggg caatcacaac agtatatgat gtattttcgc tagtagttct aactatgctt
    14101 tcgctaacat tcaattgttt caagaaatta agaatatcct caccaaagaa atcttttcct
    14161 atttttccaa ttagttggca attcaatccc ataatgctta atgaaattcc aacatttggc
    14221 acgactccac cagcagcaat ctttaccccc atcatattag tcaaatgacc tggtgtaaaa
    14281 acctgttgaa tatccaaata attatctgtc ctgaaaacgg gagttatgtc tagacatata
    14341 tgtccagcta ccattacatc atttactttt ttggggattc ctacattcag atcattttat
    14401 ttatgaaatg ttataactta tcataatcgt ttaccagcgg atgtaagata gcctcatcct
    14461 cttcaataga agaaaacctt ctcgcttgtt caacaaaaaa attgtctgtc ttgtcgtcgt
    14521 ttatggatga cacttcaccc acaattaagt cacctgctcc tttttttgcc caaaatgcat
    14581 gataaatgta agttgaaagc gtaatactgc aaccgggctg gatatcaatt gtttctccag
    14641 ctttaaaaca gtactttatg ccgtccataa aaacttcaac atttacatag ttatccaatt
    14701 taccttcctg gtttgaacca aacaattgga tacacatgac cccaccaccc ctgttgataa
    14761 tatcttctgt ttttatgcgg tggcaatgca ttggtatctt ctgccccttt tcgtgttgga
    14821 gtataattat tttctccgca taaggggtcc caataccatt attttttata tctccattcc
    14881 taatggtgaa aagaacagct ccaacttctt tgaaccttcc cgaaccaaaa tcggtaatgt
    14941 cccatccctg catcactcga acgatagtag ctattttatc cttattttgt tcccattcat
    15001 taattgtcca gtatgcaaag gccggtaatc tgaagttatt cctttcaagc aagtcctttg
    15061 aaatttttat ggcttcatta atctctgatc ttttcatata ataaaatttt atgactatcg
    15121 gtaatgttta acaatatttt gataatttgt agtaacacaa gcaat
//
LOCUS       DAOSPX010000038        15041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4055_length_15041_cov_3.940077, whole genome shotgun sequence.
ACCESSION   DAOSPX010000038 DAOSPX010000000
VERSION     DAOSPX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15041
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4055_length_15041_cov_3.940077"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLZ75_03330"
     CDS             <1..876
                     /locus_tag="PLZ75_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOS71465.1"
                     /translation="KSARLPIMTSCCPGWVNFFEEYFPDLNDIPSTAKSPAQMFGSIA
                     KTYFADKIKVDRKDLVVVSIMPCLAKKYECQREEFSVNGNRDVDFSISTRELAAFIKQ
                     ANIDLNNLEDGEFDAPLGESTGAGVIFGATGGVIEAACRTAYELHTGKKLERIDFTEL
                     RGLQGVRSATIDFDGLPLKIGIAHGLGNARKLLDEIKSGKSQYHAIEVMACPGGCIGG
                     GGQPPHHGNSEIIKARAAAIYEEDRNKPLRKSHENPFIIKLYDEFLGKPNSEKAHHLL
                     HTRYFDKKKKIVVKH"
     gene            911..1393
                     /gene="nuoE"
                     /locus_tag="PLZ75_03335"
     CDS             911..1393
                     /gene="nuoE"
                     /locus_tag="PLZ75_03335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOS71466.1"
                     /translation="MSSIKIELRQEDINKIKEICTSFGNDPRELINVLHKCQEHFGYL
                     PAEVQEVISDELEVPVAKIYGVVTFYSFFTMTPRGKHPISICMGTACYVRGAETVLEE
                     FRKELGLQVGQTTEDGKFSLSSLRCVGACGLAPVVMIGDKTYGRVAPDDVRNILKEYE
                     "
     gene            1489..3720
                     /locus_tag="PLZ75_03340"
     CDS             1489..3720
                     /locus_tag="PLZ75_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS71467.1"
                     /translation="MDINKTIMQHKQVCNYASQKRIKQSLDTLAGMLENVTSGYLRDE
                     YDNLVMTYRNMLAYTIEGIRDPERNKIYQKLIQSILKLSDRVRQDILSHNSGWNTYWL
                     KQQTEKEQQLTGKTIVETVDDLMFKSELDEWLRLSSETNPDPESAIAIKHKKLISNIF
                     NHLWLTDYYGEAEESLINILLNSGKFRWYESSVFTTAITLSSFRTWQTEKLLILARIY
                     RSGQEQVMERALSGLLLNFHYHNDRIMLYPEVTEAINSLTKLPAFREHCRLIVLQIIR
                     SRETEKLSRRLHDEILPQVAKLRPGIEEKLDLNNILPTDKNEGKNPDWSAMFSGADEI
                     YRTMEELTNLQMEGADVYMSAFANLKNFDFFRDIQNWFMPFHPDHEVVEEIFQDDILG
                     QGTNELAEAMYKTPFICNSDKFSLILNLKYLPATQKSMMLKVFRMELEGFQQLNEDEF
                     LTDPNKRFRINITQYLQDIYRFFKLSPYKKEFEDIFTGKLDIYNSHFFRLSCDTGEAE
                     AGLADYFFSKDFYEDALVLYLRMLENRPDDAQLYEKTAYCHQENGNYREALRYYKKAE
                     LIDRKAWTVKKIGLCYRRLGRNEEALEYYIQAGDMEPDNLHTTMMTGHCYLDLRQYEN
                     ALKYYFRIEYKDPGNLKILRPIAYCYLALGRFEESEKYYELLSAGKLNAHDLINMGHL
                     ALARGRKKEAVELYRKSILSGEINKKNFISIFTEDQDQLISLGVSADDLPILLDYLLF
                     ILE"
     gene            complement(3655..4239)
                     /locus_tag="PLZ75_03345"
     CDS             complement(3655..4239)
                     /locus_tag="PLZ75_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOS71468.1"
                     /translation="MKTKPIILVVLTLIIGFVLGMLTSAQLRLNRLKPVRFYFSDERF
                     REGFYKIIQPDEKQKAEIDKILDKYNKKNRELQEYFWKESESNMRAFRKEIDSKLTRE
                     QISRLREMDERREKMIRDARRRHRHDSLGHQDSGAGDRRFRQFDDAGRIPEGNRPPPR
                     ERGPAAPSEDPRPSFQNKQEVVKQDRQIIRTDPQ"
     gene            complement(4250..4576)
                     /locus_tag="PLZ75_03350"
     CDS             complement(4250..4576)
                     /locus_tag="PLZ75_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71469.1"
                     /translation="MIRSLNPEADAADIARQLENAGVSYDFGNDFTARVSEKLFSQGR
                     VIPFEPDFVTNLSSVFYRIAVTGVAAIILLLISIFIMEGSLSVNSFLGITDTFDESIV
                     YLLTGN"
     gene            complement(4609..5166)
                     /locus_tag="PLZ75_03355"
     CDS             complement(4609..5166)
                     /locus_tag="PLZ75_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOS71470.1"
                     /translation="MKVPYSKADESELIKATLGNDELAFGEIVNRYRKMVARTVKGML
                     GDTIYAEDIGQEVFIKLYHSLAEFRGESRLATYIQKIAINLTLNEIKRRKRFFSLFAQ
                     KGSDEMYEMDIADGNCEEKREAGEIVAKALKKLDPKFRVVVAMRMLQGYSTKETAEML
                     ELPVGTVLSRLSRAQEQMKKIIEKL"
     gene            complement(5267..7357)
                     /locus_tag="PLZ75_03360"
     CDS             complement(5267..7357)
                     /locus_tag="PLZ75_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOS71471.1"
                     /translation="MPASADIFAQIVIERSKDKVIISGIQYYIHLVKKGETAYSISKA
                     YNVTLEELNRENPSAVSGLKEGQSLKIPVREAVPASQASPSSPLSPPSQASPASPPSP
                     ATGQQPAMQRDESKYYYHRIQPGQTIYSLSKLYDVPEAEILAANPGIDISRLPVYSEI
                     AVPKKETLAGRESAPVRDGAAPQETGKTSFIRAVSDSRTVRDTRELQDKRYMFHKVEA
                     GESLASIAEKYGVTLRELRRENRNVKFPQVGDYLRIPESGRMKFQLAEAEQPAADTSD
                     VQPADTLMIYERPEGYTPVGRLAGTFNVAVLLPFYLAENAIRMEIDSSKYVKGKVTYR
                     PVKRLEDWIYYRSAGFVEMYEGILLAADTLRSLGLNINLHAFDIKSDTIEVTRLINRG
                     QLAGMDLIIGPVYSRNLAIVSEYARKLNIPVVSPVRLMNNSALVNNPLLFMANASLEV
                     AQDAIAREASDYYLNNFIFIHADTSRNDPDVGRFRQKILSELSSRIPRSEIRFRELAF
                     YSRSAFNSDSVRRLENALSARSENLIIIASEDDPVVSETLQEIHSLSKKYPVRIIGYP
                     SMRGIENLDPKFIFELGVLVLSPFWIDYSEADVSGFIRGFRKKFLTEPSEMSYAWIGY
                     DLTYYFLSGLAIHGRDFLSYPWMHNPDLLQTEFDYRRKGPGDGFENYKLYRVKYTQDY
                     EVVLEAGEPEYRKR"
     gene            complement(7502..8470)
                     /locus_tag="PLZ75_03365"
     CDS             complement(7502..8470)
                     /locus_tag="PLZ75_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOS71472.1"
                     /translation="MTEIVIFLEDIDPVIFFGTNNLLLDRIRTFFPKIKILSRGNEIK
                     CLGEESEITLFAERFNELIDYYRKYNRIEEQDLEKYLTDPEHMKSAASGDFEKVLVFG
                     TSGKPVRARTVNQLLLVNDYSINDLIIAEGPAGTGKTYTSIALAVRALREREVKKIIL
                     TRPAVEAGERLGFLPGDMKEKLDPYLQPLYDALHDMIPFRKLETWLEDGTVQIAPLAF
                     MRGRTLESAFVILDEAQNATISQLKMFLTRMGINSKFILTGDTTQIDLPKKSESGLLQ
                     AMRILGGIEGVSVIKFDERDIVRHKLVKRIVKAYEEEENGAAEKET"
     gene            8616..9410
                     /locus_tag="PLZ75_03370"
     CDS             8616..9410
                     /locus_tag="PLZ75_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOS71473.1"
                     /translation="MPLVTLTTEWKPDDIYSGIIKGKLSSLYPGVTIVENASGIAAFD
                     ILHAAFVVRNTYLNYPEGSVHIICVHSEPVKKQDHLIVRAKGHFFIGTDNGIFSLILN
                     GEPDEIIKIDHDEDGDELDVFSKTAASLLSGRNASELGKPKAELVERMPLRATIESNT
                     ITGSIIFIDSYGNAISNITREVFLRIFEKDDYRILIQSNKNYTRQIVRKYSEVAVGDL
                     LTRFNSLGLLEIAINGANISQLLSLGIGTVVRIEKVKETTAPGHLF"
     gene            9423..10205
                     /gene="mazG"
                     /locus_tag="PLZ75_03375"
     CDS             9423..10205
                     /gene="mazG"
                     /locus_tag="PLZ75_03375"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOS71474.1"
                     /translation="MNDRKDSVKEFERLMEIMDRLRESCPWDKKQTNETLRKLTIEET
                     YELADAIISGDDDGIRNELGDLMLHIVFYAKIGSEKGVFSMKDILRGINEKLIYRHPH
                     VFSDVRVSGAEEVEENWEQLKIRENNGNKPVLSGVPSSLPSMVKANRIQEKVRGVGFD
                     WEKPEQIWDKVLEEISELKSEIERYDREKIESEIGDVIFSVINASRLYGIDPEAALEK
                     TNRKFIKRFNYLEKETISRGQSLHDMSLDEMNRIWEKAKKHD"
     gene            complement(10239..11102)
                     /gene="speB"
                     /locus_tag="PLZ75_03380"
     CDS             complement(10239..11102)
                     /gene="speB"
                     /locus_tag="PLZ75_03380"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOS71475.1"
                     /translation="MNFGGPEIVSAYAESEIVMVPVPYDETSTWIKGSDRGPGAIFEA
                     SPNLEFYDMETGTEVWRRGIFTASPVTEKRSPELLVKAVYERALSLLNEKKFPVIIGG
                     NHTVSIGAARAFAGHYPDLTVLQLDAHADLRQEYDGSPYNHACVMARIRESVPVVQVG
                     IRSMSAEELPYADRSRIFPAHELYADRELYGKALSLLSPDIYITIDLDVLDPSIMPST
                     GTPEPGGPGYRELMDFLREVAVKRNIVGFDVVELCPSPSNKAPDFVAAKIIYQLLSYI
                     FALKDHSPANP"
     gene            complement(11152..11619)
                     /locus_tag="PLZ75_03385"
     CDS             complement(11152..11619)
                     /locus_tag="PLZ75_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOS71476.1"
                     /translation="MLLKDLMAIPGQPGLFRFIAQGKNAIIVEHLETRKRSTVFSSAR
                     ASSLEEISVFTENEDLPLGKVFDKIHEKENGGPAIDHKSDPEKLRSWFGEILPDYNRE
                     RVYVSDIKKIIQWYNTLHKLDLLVREETAGSVEDGESEASATDADAVTGTDAS"
     gene            complement(11728..12459)
                     /locus_tag="PLZ75_03390"
     CDS             complement(11728..12459)
                     /locus_tag="PLZ75_03390"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOS71477.1"
                     /translation="MARLGKWIGGGLGWVMGGPIGALLGFLLGSVVDSASDYIRPEIA
                     ETSRKTTPGGFGVTFIVLIAAVMKADGKVLKSELDYVKQYFVRQFGRETAREATIMLR
                     DLLKQNIPLADVCRQIERHMDYSSRLQLLHLLFNVSIADGVVNQAEQAVIEKIAAYTE
                     VSSADYISIKNMFIPDTDPDYKILETDPSATDEEVKKAYRRMAMKYHPDKVSHLGEDF
                     KKTAGEKFRKVNEAYKRIKEERNMK"
     gene            complement(12468..13067)
                     /gene="coaE"
                     /locus_tag="PLZ75_03395"
     CDS             complement(12468..13067)
                     /gene="coaE"
                     /locus_tag="PLZ75_03395"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOS71478.1"
                     /translation="MKKRMKLGITGGIGSGKTSVCRVFGILGIPAFFADPEAQFLMNS
                     EDYIIRGINDIAGKDMYPEGVLNREELAGIIFNNPEKLKRVNSLVHPAVFERFRNWAE
                     NQTSPYVILEAAILFESGGAGHVDRIATVTAPLEERISRLTAGNKLTREQVAERIRHQ
                     MDDEERISLSDYVINNSENEMIIPVILDIHEDILAHLDI"
     gene            complement(13126..13593)
                     /locus_tag="PLZ75_03400"
     CDS             complement(13126..13593)
                     /locus_tag="PLZ75_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71479.1"
                     /translation="MEKSEKNWSGRLAKKGMGNLKANILVFSIFLFMSFVFWYLNSLG
                     KTIQSDIRYPVKFINVPRDRELETVPARLNLVLQGTGFSMLRLKISGSRDPAVIDLLK
                     VPYKSVRSNAPADYYLVTSGLVQNFNTQLKPECRITAIRPDTIFFSFKEVVEK"
     gene            complement(13623..13925)
                     /gene="yajC"
                     /locus_tag="PLZ75_03405"
     CDS             complement(13623..13925)
                     /gene="yajC"
                     /locus_tag="PLZ75_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOS71480.1"
                     /translation="MSQPAGAAGGGQSNPMITFLPLILVFVVFYFFMIRPQMKKQKEM
                     NTYRSSLKKGDKVVTTGGIYGRVHEVKDNYIMMDAGGDVKLKVDKSALLKDPSADE"
     gene            complement(13980..14951)
                     /gene="nusB"
                     /locus_tag="PLZ75_03410"
     CDS             complement(13980..14951)
                     /gene="nusB"
                     /locus_tag="PLZ75_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOS71481.1"
                     /translation="MISRRLLRIKALMALYAFNRREDGDLVKAESELVFSIAKTYDLY
                     HYLLLLVLEISDVATDRIERALQKKMPTPQDLNPNRRFIDNPVIVQLRNNRSLNKYLN
                     GKKLSWVNFAHIPRILFNNMLSWDEFGEYMNQEEMNYQGHKKFVINLVSKLFSASEDL
                     VNSLEEQSIYWNDDLEYVLLMIEKTLKKFRAASGEQAELMPLYKNREDEEFVKLLFRK
                     AVLNYRQSSELIDNNTTNWEVDRIALMDTLVMQLAIAEVLEFPEIPVKVTLNEYIEIA
                     KYYCTPKSSTFVNGILDNIFREMREKGLLKKYGRGLIGESTDSKLNN"
BASE COUNT         4132 a         3506 c         3159 g         4244 t
ORIGIN      
        1 aagagcgcca gactgcccat aatgacttcc tgctgtccgg gatgggtgaa cttctttgaa
       61 gagtatttcc cggaccttaa tgatataccc tcaacggcca agtcgcctgc ccagatgttc
      121 ggctccattg caaaaacata tttcgccgat aaaatcaagg tcgaccggaa ggatctggtg
      181 gttgtatcaa taatgccctg ccttgcaaaa aaatacgaat gccagagaga ggaattctcc
      241 gtcaatggaa atagggatgt cgatttttca atttcaacca gggaacttgc agcttttatc
      301 aaacaggcca atattgatct gaataatctt gaggatggtg aattcgatgc tcctctgggc
      361 gaatcgacgg gtgctggggt tattttcggt gcaaccggcg gtgtcattga agctgcatgc
      421 agaacagcct atgaattaca cacaggaaag aaacttgaaa ggatagattt tacggaattg
      481 agaggcctcc agggagtgcg ttctgccaca atagatttcg acggacttcc cctgaaaata
      541 gggatagccc acggacttgg aaatgccagg aagctccttg acgagataaa atcaggaaaa
      601 tcacagtacc atgccataga agtaatggca tgccccgggg gttgtatcgg aggagggggc
      661 cagcccccgc atcacggcaa ctctgaaatc ataaaagcca gggctgcagc aatatatgaa
      721 gaagacagaa acaaaccgct gagaaaatca catgagaatc ctttcatcat aaagctttat
      781 gatgaattcc tcgggaaacc aaacagcgag aaggctcatc atctgcttca tacaaggtat
      841 ttcgacaaga agaaaaaaat tgtagtaaaa cattaagcca gttatttgat ccatcataaa
      901 tcctatcgtc atgtcaagca tcaaaataga attaaggcaa gaagacataa acaaaataaa
      961 ggagatatgt acttcattcg gaaatgaccc gcgtgagctt atcaacgtcc tccataaatg
     1021 tcaggaacat ttcggatatc tccccgctga agttcaggaa gttatttctg acgaactgga
     1081 agttccggtg gcaaagatct acggagtggt aactttctat tcctttttca ccatgacgcc
     1141 caggggcaaa cacccgataa gcatctgtat gggaactgcc tgctatgtca ggggagctga
     1201 aacagtactt gaagaattca ggaaggaact tggcctgcag gttggacaga caacggaaga
     1261 tggcaaattc tcgctttcaa gtctgagatg tgtgggagcc tgcggcctgg ccccggttgt
     1321 aatgattggc gataagacct acggccgcgt ggcgcctgac gatgtaagga acattctgaa
     1381 agaatacgaa taatccggaa ttcctatttg cggcctgatt gttgcagtat caatcatgac
     1441 aatatccatg tgatattttg tcatgatttg tttttataac ggatactgat ggatatcaat
     1501 aaaaccataa tgcagcataa acaggtttgc aattatgctt ctcagaagag aatcaaacaa
     1561 agccttgata ccctggcagg tatgctggaa aatgtcacat ccggttattt acgggatgag
     1621 tatgacaacc tggtcatgac ctacaggaat atgctcgcct atactattga agggatcagg
     1681 gaccctgaaa gaaataaaat ctaccagaag ctgatccagt caatcctgaa gctttctgac
     1741 cgtgtcaggc aggacatatt gtcacataat tccggctgga acacatactg gctcaaacaa
     1801 cagactgaga aggaacagca gctcacagga aaaaccattg tagaaacagt tgatgacctg
     1861 atgtttaaat cagaacttga tgagtggctc aggttaagca gtgaaacaaa cccggatccc
     1921 gaatcagcca ttgcaataaa gcataagaaa ctgataagta atattttcaa tcacctgtgg
     1981 ctgactgatt attatggtga ggccgaggaa agccttatca acatcctcct taattccgga
     2041 aaattcagat ggtatgaatc gagcgtcttt accacagcca taacactcag ttcattcaga
     2101 acgtggcaga ccgagaaact gctcatcctg gcaaggattt acaggtccgg gcaggaacag
     2161 gtaatggaaa gggcactgtc gggcctcctg cttaactttc attatcataa cgacaggata
     2221 atgctttacc ccgaagttac ggaagcaatc aacagtctga caaagcttcc ggcattcagg
     2281 gagcattgca ggctgattgt ccttcagatc atcaggtcac gcgaaacaga aaaactgagc
     2341 cgccgcctgc atgatgaaat tctgccgcag gtggcaaagc tccggcccgg aatagaggaa
     2401 aagctcgatc tcaataatat cctgccaacc gacaagaacg agggaaagaa ccccgactgg
     2461 tcagcaatgt tcagcggtgc ggatgagata tacaggacaa tggaggaact taccaacctt
     2521 cagatggaag gagctgacgt ttacatgtcg gcttttgcca atcttaagaa cttcgacttt
     2581 ttcagggata ttcagaactg gttcatgcca tttcatcccg atcatgaagt ggtggaggaa
     2641 attttccagg atgatattct cgggcagggt acaaatgaac tggccgaagc aatgtacaaa
     2701 acgcccttta tctgcaattc tgacaagttc tcactcatcc tgaacctcaa gtaccttccg
     2761 gcgacacaga aaagcatgat gctgaaggta ttcagaatgg aactggaggg attccagcag
     2821 cttaatgaag atgaattcct gaccgatccc aacaaacgtt tccggatcaa cattacccag
     2881 tatcttcagg atatttacag atttttcaaa ctgtctccct ataaaaagga atttgaggat
     2941 atttttaccg ggaagctgga tatttataat tcccatttct tccgcctttc ctgtgacacc
     3001 ggggaagcgg aagccggcct ggcagattat tttttcagca aggatttcta tgaagatgcc
     3061 cttgtccttt acctcagaat gttggaaaac aggccggatg atgcacagct ttatgaaaaa
     3121 acagcctatt gccatcagga aaacggaaac tacagggaag ccctcagata ttataaaaaa
     3181 gcggaactca ttgacagaaa agcatggaca gttaaaaaga tcggactgtg ttacaggcgg
     3241 ctggggagaa atgaggaagc cctggaatac tatattcagg caggtgacat ggaaccggac
     3301 aatctgcata caacaatgat gacgggacat tgttaccttg atctcagaca gtatgaaaat
     3361 gcccttaaat attatttcag gatagagtac aaggatccgg gaaatctgaa aatcctccgc
     3421 cctattgcat actgttacct tgccctgggc cgctttgagg aatcggagaa atactatgaa
     3481 ctcctttcag ccggaaagct taatgcccat gaccttatca acatgggtca cctggctctt
     3541 gccaggggca ggaagaaaga agcagttgaa ctttacagga aaagtatact gagcggcgaa
     3601 ataaacaaaa aaaacttcat ttcaattttc acggaagatc aggaccagct gatctcattg
     3661 ggggtcagtg cggatgatct gcctatcctg cttgactacc tcctgtttat tctggaatga
     3721 tggtcgggga tcttcagaag gggcagcagg tcccctttcc cggggaggag gacggtttcc
     3781 ttccggaata cggcccgcat catcgaactg cctgaatctt ctgtctccag caccagaatc
     3841 ctgatgaccc agagaatcat gcctgtgcct tcgcctggca tccctgatca ttttttcgcg
     3901 cctttcatcc atttccctca gcctggagat ctgttccctt gtaagtttgg aatctatctc
     3961 cttcctgaat gccctcatgt tggattctga ttccttccag aaatattcct ggagttccct
     4021 gttcttttta ttgtatttgt cgaggatctt atcaatttct gctttctgct tctcatcagg
     4081 ctgaataatt ttataaaaac cctccctgaa tctttcatca gaaaagtaga atcttaccgg
     4141 tttaagcctg tttaatctca gttgtgcaga agtgagcatg cccagcacaa atcctataat
     4201 gagagtcaga acaacaagaa ttatcggttt tgtcttcatg attccggaat taattacccg
     4261 taagcaggta tacaatactt tcatcaaagg tatcagtgat acccaggaat gagtttaccg
     4321 aaagcgatcc ctccatgatg aaaatagaaa tgaggagaag tataatggca gccactcctg
     4381 ttactgcgat cctgtaaaag accgagctca gatttgtaac aaagtccggt tcaaatggaa
     4441 tcactcttcc ctgagagaat aatttctctg acaccctggc tgtaaaatca tttccgaaat
     4501 catacgatac tcccgcgttt tccagctgtc tggcaatatc tgccgcatca gcctcaggat
     4561 tcagagatct gatcatcagt tgttttatat ccggacattt cataacacct ataatttttc
     4621 aattattttt ttcatttgtt cctgtgccct tgaaagcctt gacagaaccg ttccgacagg
     4681 aagctcaagc atttcggctg tttctttggt ggaataaccc tgcagcatcc tcatcgccac
     4741 aacaactctg aatttcggat caagcttttt aagcgccttg gccacaattt cccctgcttc
     4801 acgcttttcc tcacaatttc cgtccgcaat atccatttca tacatttcgt cactgccctt
     4861 ttgtgcaaac agggaaaaaa accttttcct cctttttatt tcattcagcg taaggtttat
     4921 cgcaattttc tgaatgtagg ttgccagcct tgattccccc ctgaattcgg ccagggaatg
     4981 ataaagcttt ataaacactt cctgaccaat gtcttcagca tatatcgtat ctccaagcat
     5041 acccttgaca gttcgtgcaa ccattttgcg gtacctgtta acaatttccc cgaatgcaag
     5101 ctcgtcgttc ccgagagtag cctttataag ttcggactcg tcggccttgc tgtacggtac
     5161 tttcattgtt atccttttta tagacaatac tgaaggaaag attattcccg attgttaatt
     5221 tttattaaca taaaaacagg cggcctggct gatattcatg aattccttac cgtttccggt
     5281 attccggctc accggcttcc aggactacct cataatcctg tgtatatttc accctgtaaa
     5341 gtttatagtt ttcgaatccg tcacccggac ctttcctcct gtaatcaaat tccgtctgaa
     5401 ggagatcagg attatgcatc cagggatatg aaaggaaatc cctcccgtgt atggccagtc
     5461 cgctcagaaa ataataggta aggtcgtaac cgatccatgc atagctcatt tcagatggtt
     5521 cggtcaggaa ttttttcctg aaccccctga tgaatccaga cacatccgct tctgaataat
     5581 ctatccagaa aggtgataaa accagtacgc caagttcaaa aatgaatttg ggatcaagat
     5641 tttctattcc cctcattgaa ggatatccta ttattctcac cggatatttt tttgacagtg
     5701 aatgtatttc ctgcagggtc tcacttacca cgggatcatc ttccgatgca atgataatca
     5761 ggttttccga tctggcagaa agagcattct ccagccttct gaccgaatcg ctgttaaaag
     5821 ctgatctgct gtaaaaagcc agttccctga acctgatctc gcttctgggg attctgctgc
     5881 tcagctcaga taatatcttt tgccggaaac ggccgacatc gggatcgttc cttgaagtat
     5941 ccgcatgaat gaatataaaa ttgttaaggt aatagtcact tgcctccctg gctattgcat
     6001 cctgggcaac ctcaagggat gcatttgcca taaagagcag ggggttattg accagggccg
     6061 agttattcat aagccggacg ggtgacacca ccgggatatt cagtttacgg gcatattccg
     6121 acacaattgc cagattccgt gaataaaccg gaccaattat caggtccatt cctgcaagct
     6181 ggccccggtt tatcagcctt gtcacttcta tggtatcact cttgatatca aatgcatgca
     6241 gatttatatt caaacccagg gagcgaaggg tatcggctgc aagcagtatt ccctcgtaca
     6301 tttcaacaaa accggcactc cggtaatata tccagtcttc aagccttttc accggccggt
     6361 aggtaacctt gcccttcaca tactttgatg aatcaatttc cattctgatg gcattttctg
     6421 caagatagaa aggcaggaga actgcaacat tgaaggttcc tgccagcctg cctaccggtg
     6481 tatagccttc gggtctttca tagatcataa gggtatcagc cggctgaaca tcgcttgtat
     6541 ctgccgcagg ctgctcagcc tcggccagct gaaatttcat ccgccccgat tccggtattc
     6601 tgagataatc cccgacctgg ggaaatttga cattccggtt ttcgcgcctc agttccctta
     6661 gtgtgacacc atatttttca gctattgatg caagggattc cccggcttcg accttgtgaa
     6721 acatatatcg cttgtcctgt aattccctgg tatcccgtac ggttctggaa tccgaaactg
     6781 cgcggatgaa gctggttttt ccggtttcct gcggggctgc gccatccctg acaggagctg
     6841 attccctccc tgccagggtc tcctttttcg gaaccgctat ttcagaataa accggaagcc
     6901 gtgaaatatc aatacccggg ttagctgcca gaatttccgc ctcgggaaca tcataaagtt
     6961 ttgacaatga ataaatagtc tgaccaggct gaatcctgtg ataataatat ttcgattcat
     7021 ctctctgcat ggcaggctgc tgtccggttg ccggggatgg aggggatgcc ggggaagcct
     7081 gggatggagg agacaacgga gatgaagggg atgcctgaga cgccgggact gcttccctca
     7141 ccgggatctt cagcgactgt ccttccttaa ggccggagac agcagacggg ttttcccggt
     7201 taagttcttc cagggtgaca ttgtaagcct ttgatattga atatgcggtt tctcctttct
     7261 taaccaggtg tatataatac tggatcccgg atattattac tttatccttc gatctctcaa
     7321 tcactatctg tgcaaatata tctgcagaag caggcaaaag gattaaaata attgtcagaa
     7381 ataatcttaa atgtacttta atattcatta ttagacactt attcattatg atatcattgc
     7441 aaagttagca gataattcag aatcagcgtt aaaagctgcc tgaagcatca caccatgtga
     7501 tttatgtttc cttttccgcg gcaccgtttt cttcttcttc ataagcttta acgatccgct
     7561 tgacaagttt atggcgcact atatcccttt catcgaactt tattactgac actccttcaa
     7621 ttccccccaa tatcctcatt gcctgcagaa gtccggattc actctttttc ggaaggtcaa
     7681 tctgggttgt atcaccggtc agtatgaatt ttgagtttat ccccatcctt gtaagaaaca
     7741 ttttcaactg actgatagtg gcattctggg cctcgtcaag gataacgaat gcgctctcca
     7801 gtgttctgcc gcgcataaat gccaggggag ctatctgaac cgtaccgtct tcaagccagg
     7861 tttccagttt cctgaaagga atcatgtcat gaagggcgtc atacaatggc tgaagatagg
     7921 gatctagctt ttccttcatg tcacccggca gaaatccaag tctctctccc gcttccacgg
     7981 ccggccgcgt aaggataatc tttttaacct ctctctctct taacgccctg acggccaggg
     8041 ctattgaagt atatgtcttt ccggttcccg ccggaccttc ggcaatgatc agatcgttga
     8101 tactgtaatc gttcacaagc aacagctgat tcaccgtccg tgcccttaca ggttttccgc
     8161 tggtgccaaa aaccaggacc ttttcaaaat caccgcttgc cgcacttttc atatgctccg
     8221 gatccgtcag gtacttttcc agatcctgtt cttcaatacg attgtatttc cggtaataat
     8281 caatgagttc attgaacctt tcggcaaaca gcgttatttc agactcttca cccaggcatt
     8341 ttatttcatt cccccttgac agaatcttga ttttaggaaa aaatgtccgg attctgtcaa
     8401 gcaataaatt attggtgcca aaaaaaatga ccggatcaat atcttcaaga aaaattacta
     8461 tttccgtcat caattataac ctttcatttt caatgggcag gaaataatta tcaactgcct
     8521 gctcagggta aagttcataa ataatcatca tggcagcgat acaaagtaag tttatttttc
     8581 catagaaggt tatctttgtt ttaagttttt tggatatgcc gcttgttact cttaccactg
     8641 aatggaaacc tgatgatatc tactccggaa tcataaaggg aaagttatca agcctgtacc
     8701 cgggagttac cattgttgaa aacgcatccg gcatagcggc atttgacata ttgcatgccg
     8761 cctttgttgt cagaaacaca tatctcaact atccggaggg ctctgttcac atcatatgcg
     8821 ttcattccga accggtaaag aaacaggatc atctcatagt cagggcaaaa ggccattttt
     8881 tcataggaac tgacaacggg attttcagtc ttattctcaa tggggaaccg gatgaaatca
     8941 taaaaattga tcacgatgag gatggggatg aactggatgt tttttctaaa acggctgcat
     9001 ccctgctttc aggcaggaat gcttccgaac tgggtaaacc gaaagctgaa ctggttgaac
     9061 gtatgcctct gagagctaca attgaaagta ataccattac aggcagcatc atttttatcg
     9121 attcctacgg aaatgccata tcaaatatta ccagggaggt atttctcaga atttttgaaa
     9181 aggatgatta ccgcatcctg atacagagca ataagaatta tacccggcaa atcgtcagaa
     9241 aatacagtga agtggctgta ggggatcttc ttacaagatt caattccctg ggcctcctgg
     9301 aaatagcaat aaacggtgca aatatttctc aattattaag tcttggcatc gggaccgtgg
     9361 tcagaattga aaaagttaag gaaaccacgg ctccgggcca tcttttttaa aaatccggaa
     9421 taatgaacga cagaaaggac agtgttaagg aattcgaaag gctcatggaa atcatggaca
     9481 gattaaggga atcctgtccg tgggacaaga agcagaccaa cgaaacactc cgcaaactaa
     9541 ccatagaaga gacctatgaa cttgcagatg ccataatttc cggcgatgat gacgggataa
     9601 ggaatgagct gggagacctg atgcttcata ttgtgttcta tgcaaaaata ggcagcgaaa
     9661 aaggcgtgtt cagcatgaag gatattctca ggggaatcaa tgagaaactt atttacaggc
     9721 atcctcatgt tttcagtgat gtcagggttt caggagccga agaggttgag gaaaactggg
     9781 aacaactgaa gatcagggaa aacaatggaa acaagccggt tctttcgggt gtgccttctt
     9841 cccttccgtc aatggtaaaa gccaacagga tacaggaaaa agtgagagga gtgggattcg
     9901 actgggagaa acccgaacag atctgggaca aggtgctcga ggaaatatca gaactgaaaa
     9961 gtgaaattga aaggtacgac agggaaaaga ttgaatcaga gatcggggat gtcatttttt
    10021 ctgtaatcaa tgcttcccgt ctttacggaa ttgatccgga agcagctctt gagaaaacaa
    10081 acagaaaatt cataaagaga ttcaactacc ttgagaagga aaccatttcc aggggacaat
    10141 cacttcatga catgagcctc gacgagatga acaggatctg ggaaaaggca aaaaagcatg
    10201 actgatcccg gatcaggttt ccggggcatg ctgtaaactc agggattcgc cggggaatgg
    10261 tccttcaatg caaatatata gctcagaagc tgatagatta tctttgccgc aacaaaatca
    10321 ggggccttgt tcgagggaga agggcataat tcaaccacat caaatccaac aatgtttctt
    10381 tttactgcca cttccctgag aaaatccatc agctcacggt atcccggccc cccgggttca
    10441 ggagtaccgg tagaaggcat tattgaagga tccagtacat ccaggtcaat tgtaatatag
    10501 atatcaggtg aaagaagact gagggccttt ccgtatagtt cacggtcggc gtacagttca
    10561 tgtgcgggaa atatcctgct ccggtcggca tagggcagct cttctgccga catgctccgt
    10621 attccgacct gtacaacggg aaccgattcc cttatcctgg ccattacgca ggcatgatta
    10681 tagggcgatc cgtcatattc ctgcctcaga tcggcatggg catcaagctg cagaaccgta
    10741 agatcagggt aatgaccggc gaaggccctt gctgctccta tgctaacggt gtgattgccg
    10801 cctatgatta ccgggaattt tttttcattc agaagagaca gagctctctc ataaacagct
    10861 tttactagta attccggaga tcgtttttca gtaacgggac tggcagtgaa gatccccctt
    10921 ctccagacct cagtgcctgt ttccatatca taaaattcaa ggttcggcga tgcctcaaat
    10981 atggcaccgg gtcccctgtc agagcctttt atccaggtac ttgtttcatc atatggtaca
    11041 ggcaccatta caatctctga ttcagcataa gcggaaacta tttccggtcc tccgaaattc
    11101 atagttctga ttttgttttc tgatttacta tcctcactgc atcacccgga ttcaggaggc
    11161 atccgtaccg gtgactgcat ctgcgtccgt ggcggaagct tcactttccc cgtcttctac
    11221 ggagccggca gtttcttccc tgacaaggag atccagttta tgaagcgtat tataccactg
    11281 tataattttt tttatgtcac taacatatac tctttcgcgg ttataatcag gaaggatttc
    11341 cccgaaccac gatctgagct tttccggatc ggatttatga tcaattgcag gtcctccgtt
    11401 ttccttttca tggatcttat cgaagacctt tccaagcgga aggtcttcat tctcagtaaa
    11461 taccgaaatt tcctccaggg agctggccct ggcagaactg aacacagtac ttcttttcct
    11521 tgtttccaga tgttccacga ttattgcatt tttaccctgt gcaataaacc tgaacagtcc
    11581 cggctgaccg ggtatagcca taagatcctt taaaagcata tttataaatt taagttcatt
    11641 ttatttccgt ttactttact tccggttact tcatattccg ttcacttcat ttctgcttac
    11701 ttcatttccg tttaattcat gttccgttca cttcatgttc cgttcttcct tgattctttt
    11761 ataagcctca ttaaccttcc tgaatttctc accggcagtc tttttgaaat cttcaccaag
    11821 gtgacttacc ttatcgggat gatatttcat ggccattcgc ctgtaagctt tttttacttc
    11881 ctcatcagtg gccgagggat ccgtttcaag gattttgtaa tccggatccg tatcgggtat
    11941 gaacatgttc tttatcgaaa tgtaatcggc tgaggaaact tccgtataag cggctatttt
    12001 ttcaatgacg gcctgttcgg cctgattgac cacaccatcg gcaattgata cattaaacag
    12061 taaatgaagt agctgaaggc gtgaggaata atccatgtgt ctttcaatct gcctgcatac
    12121 atctgccagc ggaatattct gcttcagcag atcccttagc attattgtgg cttcacgtgc
    12181 tgtttccctg ccaaactgtc tgacaaaata ctgtttcaca taatcaagct ccgacttcag
    12241 aactttgccg tctgctttca tcacggcagc aatcaggact atgaaagtga caccgaatcc
    12301 tcctggtgtg gttttccggc ttgtttcagc aatttcgggc ctgatataat ctgaggcact
    12361 gtccactacc gatcccaaca gaaaacccag taatgcacca atgggccctc ccattaccca
    12421 tcccagtccg ccccctatcc atttgcccag ccgagccata attgtcttca gatatctaaa
    12481 tgagcaagga tatcttcatg gatatcaagt attacaggaa taatcatttc attttcggaa
    12541 ttgtttatca cataatccga caatgagatt ctttcctcat catccatctg atgtctgatc
    12601 ctctcagcaa cctgttcccg ggtaagcttg tttcctgcgg tcagtcttga tatcctttcc
    12661 tcaaggggtg ccgttaccgt cgcaatcctg tcaacatggc ctgcaccgcc gctctcaaac
    12721 agtatggctg cttccagtat cacatagggt gaagtctgat tctccgccca gttcctgaaa
    12781 cgttcaaaga ctgcagggtg aacgagtgaa ttcactcttt tcagcttttc gggattgtta
    12841 aagattattc ctgcaagttc ctccctgttc agaactccct ccgggtacat gtctttgccg
    12901 gcaatatcat ttatacccct gatgatatag tcctcactgt tcatcagaaa ttgtgcttcg
    12961 ggatcggcga agaaggccgg tatgcccagg attccaaaaa ccctgcatac agaagttttc
    13021 ccgcttccga tacctcctgt tataccgagt ttcatccgtt tcttcatacc tgtggcctgt
    13081 aaaacatatc acggacctga acatcctcag cccgtggcta tcagcttatt tttcaacaac
    13141 ttccttaaat gaaaagaata tggtatccgg tctgatcgcc gtgatcctgc attccggttt
    13201 taactgtgtg ttgaaattct gtaccagtcc tgatgtaaca agataataat cagcaggtgc
    13261 attgctccgg acacttttat acgggacttt gaggaggtct atcaccgcag gatccctgct
    13321 gccggaaatc ttaaggcgca acatggaaaa tcctgtaccc tgcaaaacca gattcagtct
    13381 ggccggaact gtttcaagct ccctgtccct tggcacattt ataaatttta caggatatct
    13441 gatgtctgac tgaatcgttt tacccagtga attgagatac cagaaaacaa atgacatgaa
    13501 aagaaagatg gaaaagacca gtatgtttgc ctttaaattg cccatcccct tttttgcaag
    13561 cctgcctgac cagttttttt cagatttttc caatgtaatt tatcccggtt gtttttcaga
    13621 tcctattcat ctgctgaggg atccttgaga agggcgctct tgtcaacctt aagctttaca
    13681 tcccccccgg catccatcat tatataattg tccttcactt catgcactct tccgtaaata
    13741 cctcccgtag taaccacctt gtcccccttt ttcagggaac tgcggtaagt attcatctct
    13801 ttctgctttt tcatctgtgg ccgtatcatg aaaaaataga ataccacaaa aacaagtatc
    13861 aacggaagaa atgttatcat gggattgctt tgtccgcctc cggcagctcc tgccggctgg
    13921 ctcatcaaat aaattaaagt taagctgatc atcattcaat tggtttatta ttgttgtttt
    13981 caattattca gtttactgtc cgttgattca ccaatcaggc ctctgccata ctttttcaga
    14041 aggccttttt ccctcatctc tctgaaaata ttgtcgagaa taccgtttac aaatgtactg
    14101 ctttttggag tacaataata ctttgctatt tcaatgtatt cattcagagt gaccttgaca
    14161 ggaatctccg ggaattccag cacttcggca attgccagct gcatcacgag ggtatccatc
    14221 agtgcaatac ggtcaacttc ccagttggtg gtattattat ctatcagttc cgagctttgc
    14281 ctgtaattca gcactgcctt cctgaaaagc agtttaacaa actcctcgtc ttccctgttc
    14341 ttgtacagag gcatgagttc cgcctgttcc ccggatgccg ccctgaactt cttcagagtt
    14401 ttttctatca tcagcagtac atattccaga tcatcattcc agtaaatgct ttgctcctca
    14461 aggctgttga caaggtcttc agaagcggag aaaagcttgc ttacaagatt gataacaaat
    14521 tttttatgcc cctgataatt catctcttcc tggttcatgt attccccaaa ttcatcccag
    14581 gaaagcatgt tgttaaaaag tattctcgga atatgtgcaa aattcaccca cgagagtttt
    14641 ttgccattca ggtatttgtt caatgacctg ttgtttctca gctgtacgat tacgggattg
    14701 tcaataaaac ggcggttcgg attgaggtcc tggggagtgg gcattttttt ctgcagggcc
    14761 cgttctatcc tgtcagtagc aacatcgctt atttcaagca caagcagtaa aagataatgg
    14821 taaagatcat aggtctttgc tatactgaaa acaagttccg attcagcttt taccagatcg
    14881 ccatcctctc tgcggttaaa agcataaagt gccatcagtg cttttattcg caataatctt
    14941 ctgcttatca ttaatataag aacctcatga tttctgagtt gcaaaaatac tctttttcca
    15001 atactcccga cacaagtttg taaaaaaaca ggatcaaagc a
//
LOCUS       DAOSPX010000039        14961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4084_length_14961_cov_4.553334, whole genome shotgun sequence.
ACCESSION   DAOSPX010000039 DAOSPX010000000
VERSION     DAOSPX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4084_length_14961_cov_4.553334"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..702
                     /gene="greA"
                     /locus_tag="PLZ75_03415"
     CDS             229..702
                     /gene="greA"
                     /locus_tag="PLZ75_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOS71482.1"
                     /translation="MSKVLHVTEGGLQKLREELDHLVAVERPLISRMLSDARDKGDLA
                     ENAEYAAAQEAQAMLEMKIARLEDIIARSRVIDESRIDNSTVRILHKVRIRNKKNNSE
                     MEYQIVPESEADLKRGKIAVNSPIAQGLIGRKNGETVEIKVPSGIMVFEIVSISL"
     gene            719..1117
                     /locus_tag="PLZ75_03420"
     CDS             719..1117
                     /locus_tag="PLZ75_03420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOS71483.1"
                     /translation="MATIFSRIIRGEIPCYKIVEDDSYFAFLDINPLKAGHTLVIPKQ
                     ETDYIFDLDDEMLAGLILFSKKIAVALKKALPCNRIGVAVIGLEVPHTHIHLVPMDSM
                     EDLNFRNPKLKFSQDEFNAIAEKIRSEIDF"
     gene            1240..1941
                     /locus_tag="PLZ75_03425"
     CDS             1240..1941
                     /locus_tag="PLZ75_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOS71484.1"
                     /translation="MNYLHDLWDDLISVLFPRLCQACGNHLMRNEKLICTECHVLIPR
                     TDFHLTDDNPLARLFWGRCRMEKAAAFSFYNRDSRIRKLIHNLKYKGVQEIGIELGRI
                     YGLILFSSGFTADMDIIVPVPLHPDKERSRGFNQSHLIARGISEVSGLALDAASLQRV
                     LTSSTQTKKSRFERWRNVEDAFLISNPAPFRGKHVLLVDDVITTGATIESCVNELIGS
                     GALKVSAAALAAALL"
     gene            complement(1969..2538)
                     /gene="ruvC"
                     /locus_tag="PLZ75_03430"
     CDS             complement(1969..2538)
                     /gene="ruvC"
                     /locus_tag="PLZ75_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOS71485.1"
                     /translation="MAVKQKAAERIILGIDPGTTITGYGVIRAGIAGPELITIGCIDL
                     SKFGDHYLKLKHIFDRTTGIIDEYHPDELAIEAPFYGKNVQSMLKLGRAQGAAIAAAL
                     SRSLPIFEYAPRKIKLSITGVGSASKEQVAAMLMKILRFRDSELKLDATDGLAAALCH
                     FYQTGRPSAAGSKAQSWKQFIRENPGRIK"
     gene            complement(2657..3547)
                     /locus_tag="PLZ75_03435"
     CDS             complement(2657..3547)
                     /locus_tag="PLZ75_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOS71486.1"
                     /translation="MNRSRQSYMLALLAVFFWSTIPTAFKICLGELGIVPMLTLASVT
                     SALALLAILAGEKKTGLIAASGRKELFNSALLGFINPFVYYLILLKAYSLLPAQVAQP
                     LNMIWPIVLVFLSVPLLGQKISRKSYIALLISFAGVYIISSRGDLLHPGRSDLKGVLL
                     ATGSSVFWALYFILNVRDKREEAVKLFLNFAFGSLYLIILFIVTELPLTGKYGLKDIL
                     SAIYIGLFEMGITFYLWLRALKTAETTAGISNLVYLAPFLSLVFIHFLLHEPLYYTTP
                     AGLLFIISGIWYQNHKASGI"
     gene            complement(3597..4871)
                     /gene="serS"
                     /locus_tag="PLZ75_03440"
     CDS             complement(3597..4871)
                     /gene="serS"
                     /locus_tag="PLZ75_03440"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOS71487.1"
                     /translation="MLTINLIREKRDFISDRLKIKNFNSEEILDKILELDSRRREIQS
                     RSDTMQAEMNRISKEIGSLMMQNRKTEAESAKENTYRLKEEIRELGEELGRLDSELRE
                     EIIRLPNLPHESVVRGSSSEDNVKVREGGEKPSVPADALPHWELIRKYDIIDFDLGNK
                     LTGAGFPVYKGKGAKLERSLISFFLDEGVSAGYTEVIPPIVVNEDSGFGTGQLPDMEG
                     QMYHMTLDNFYLIPTAEVPVTNIYRDVILNAEDLPVKNIAYTPCFRREAGSWGAHVRG
                     LNRLHQFDKVEIVQIAHPDHSYKALDEMVNHVEGLVKKLGLPYRIMRLCGSDMSFTSA
                     LTYDFEVWSAAQERWLEVSSVSNFESFQANRLKCRFREKGGKQTRLAHTLNGSALALP
                     RIVAAILENNQTPSGIRIPQALIPYTGFEMID"
     gene            complement(4990..5286)
                     /gene="rpmA"
                     /locus_tag="PLZ75_03445"
     CDS             complement(4990..5286)
                     /gene="rpmA"
                     /locus_tag="PLZ75_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOS71488.1"
                     /translation="MAHKKGAGSSRNGRDSTGRRLGVKLFGGQKVTAGNIIVRQRGTR
                     LNPGQNVGQGKDHTLFALTDGVIEFRKRKNDKVFVSVISEKETVAEKASTEQQN"
     gene            complement(5655..5951)
                     /gene="rplU"
                     /locus_tag="PLZ75_03450"
                     /pseudo
     CDS             complement(5655..5951)
                     /gene="rplU"
                     /locus_tag="PLZ75_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            6175..6768
                     /locus_tag="PLZ75_03455"
     CDS             6175..6768
                     /locus_tag="PLZ75_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOS71489.1"
                     /translation="MKRVKLKVMGISYSQTQSGAYALLLIEENGERRIPIIIGGFEAQ
                     AIVIKLENLEPPRPLTHDLFKKFADRFNIAVIEVMIYKLEDGVFFSKLLCNNGEKEYS
                     IDSRTSDAVAIALRFGCPIYISEDILEKAQISNAPSETDTVVSGESGSKRKPSSKYDS
                     FSDEELYKMIDEAIKTEDYEKAASIRDEIEKRKGKKG"
     gene            6855..8807
                     /locus_tag="PLZ75_03460"
     CDS             6855..8807
                     /locus_tag="PLZ75_03460"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOS71490.1"
                     /translation="MKQLFVFLFVLAGINAHPAPAVYKISSPDGKIAVELSAGPDIRW
                     SATYQNSEIISSVKAGLVSGDGRLSGENESVRRAQYGKIDDVLEPVVAHRHSRIEDKC
                     NTLRISFRSGLALDFRVYNDGIAYRFETALKGELIVRDEISELKFPSGSFSYYPLEER
                     FMSHNERKYIYSSLDTLTDIHLASLPVLFTTKGINILLTEADIQDYPGMWLRGMGSGG
                     LRAVWPPCPETEELRGDRDVFVTGARDYIASTAGSRTFPWRAFVIAENDGRLIESELV
                     YKLAAPLRLKDTGWIKPGKVAWDWWNANNIYGVDFRAGINTETYRYYIDFAAANGIEY
                     IIIDDGWYPLGETVLKSVPGMDVPELCRYAAERNVGVILWVAWNLFWDEIDEAVALYE
                     KWGVKGVTVDFMQRDDQKIVNFYHEAARKTAEHRLLINFHGSYKPDGMIRTWPNALTR
                     EGVMGLENNKWSSDITPSHELTIPFIRMVAGPMDFTPGAMINMGQSEFHPLFNRPSSQ
                     GTRAHQMALYVLYESPMQMLCDSPVNYMREQECTDFIVKSPVVWDDLLVLDAKITEYL
                     LLARRSGNDWFAGAITDWTARDLELDLSFLPEGEYRMEIFCDGINADRHAQDYRHLTK
                     TVRQGEKLKINLVSGGGWLAKISPIK"
     gene            8823..9617
                     /locus_tag="PLZ75_03465"
     CDS             8823..9617
                     /locus_tag="PLZ75_03465"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOS71491.1"
                     /translation="MKQYLELLEHVLADGVEKQDRTGTGTLSVFGYQMRFSLQEGFPL
                     LTTKKLHLRSIIHELLWFISGNTNIRYLQDNGVKIWNEWADGEGNLGPVYGYQWRSWP
                     AADGRRIDQLAGVIENIKNNPDSRRLIVSAWNAGELDRMALPPCHLLFQFYVSAGKLS
                     CQLYQRSCDIFLGVPFNIASYSLLTLMIAQVTGLKPGEFIHTLGDAHIYLNHLEQVKL
                     QLEREPFPLPEMEINPDVKDIDSFRYEDFTLKNYISHPLIRGEISV"
     gene            9629..10126
                     /locus_tag="PLZ75_03470"
     CDS             9629..10126
                     /locus_tag="PLZ75_03470"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOS71492.1"
                     /translation="MISIIAAVSEDLGLGRNNDLLWYIPEDLKRFKRLTYGKTVVMGK
                     RTWESLPLKPLPGRKNIVISDIPGDSFENALTACSIEDAMDKCGHDGEIFIIGGASIY
                     RQFMPYAGRLYITHVHMNKPADVFFPEIDMSRWKVVEREEYRDTGQESIPYSYIVYER
                     KEPGS"
     gene            complement(10175..11392)
                     /gene="clpX"
                     /locus_tag="PLZ75_03475"
     CDS             complement(10175..11392)
                     /gene="clpX"
                     /locus_tag="PLZ75_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOS71493.1"
                     /translation="MSKCSFCGRSKKETNILIAGLEGHICDHCIEQAHGIMTDELGTK
                     ASTRFDTINLLKPPEIKKFLDQYVIGQEMAKKIISVAVYNHYKRLTQKTDDDDVEIEK
                     SNIILVGETGTGKTLLAKTIAKMLHVPFAIVDATVLTEAGYVGEDIESLLTRLLQNAD
                     YDVKAAEKGIIFIDEIDKIARKEGDNPSITRDVSGEGVQQGLLKLLEGSVVNVPPQGG
                     RKHPEQKMIPVDTKNILFICGGAFVGIEKKIAARLNTQIVGFGASKVRSKVDLDNLLK
                     YLAPQDLRAFGLIPEIIGRLPVITHLNPLDRKALKAILTEPKNAIVKQYIKLFKMDDI
                     ELSFADDALDFIVDKAVEFKLGARGLRSICETIMIDAMYEMPSLGRDDLLITVDYAAH
                     RLSKIDMKVLRVS"
     gene            complement(11399..12076)
                     /gene="clpP"
                     /locus_tag="PLZ75_03480"
     CDS             complement(11399..12076)
                     /gene="clpP"
                     /locus_tag="PLZ75_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOS71494.1"
                     /translation="MNNSDDFRKYAGSRYGLSSLTLHRYTSVLNNYISPTIIEERQLN
                     VASMDVFSRLMMDRIIFLGLPIDDYVANIIQAQLLYLDSSDPGKDIQIYFNTPGGSVH
                     AGLGIYDTMQYISADIATICTGMAASMGAVLLTAGTKGKRSALKHSRLMIHQPMGGFQ
                     GQASDIEIQAREIMKIKKELYEIIALHTGNPLEKVEKDADRDHWMTSFEAKEYGMIDE
                     ILQKTVH"
     gene            complement(12196..13548)
                     /gene="tig"
                     /locus_tag="PLZ75_03485"
     CDS             complement(12196..13548)
                     /gene="tig"
                     /locus_tag="PLZ75_03485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOS71495.1"
                     /translation="MDISRENKDDLNAVLTVKISKSDYDERVETVLKDYRRKANIKGF
                     RPGMVPIGVIRSMYGNAVKVDEINKLISENVHKYLVDEKIDILGDPLPLTDENEKTDF
                     IHDDEFTFSFELGLAPSFELTLGKGDRVTFHEIIIDDKMKKDYADSYARRFGEFRKAE
                     ASGEKDILKGRIEAVDENGAVIPEGISADETSLSVDMVRDEEIKKSFIGLAENDSVVF
                     SIRKAFPNTAEIAGLLRMESRNAENIEGDFRLTVKEISRFYPAETGPDLYNRIYGEGV
                     VSDEEEFMQKIEEEISANLRKESEFKLMQDVRKLTLDKTEFRLPEEFLKKWLMKAGEK
                     TSSEEIENEFGTFIQDLKWQLIKNKVAKDNEIQISPEEMQEEAEKITRFQFSQYGLYY
                     ATDEQISGYARETLQREADAKRIADKILDDKVLNTLKEVVEIENKKVTAEEFNKLFEN
                     "
     gene            complement(13628..13713)
                     /locus_tag="PLZ75_03490"
     tRNA            complement(13628..13713)
                     /locus_tag="PLZ75_03490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13677..13679),aa:Leu,seq:tag)
     gene            complement(13768..14493)
                     /gene="lptB"
                     /locus_tag="PLZ75_03495"
     CDS             complement(13768..14493)
                     /gene="lptB"
                     /locus_tag="PLZ75_03495"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOS71496.1"
                     /translation="MKLHTENLVKKYHNRTVVDHVSFEVAQGEIVGLLGPNGAGKTTS
                     FYMIVGLIRPREGKIFLDGEDITDLPVYKRARKGLGYLAQEASVFRNLSVEDNLRAVL
                     EMSAFSRREQAQRLESLLDEFGLQKIRKSKGIQLSGGERRRTEIARALALKPKFILLD
                     EPFAGIDPIAVEDIQNIVSHLKNKNIGILITDHNVHETLSITDRAYLLFEGKILKSGT
                     ASQLAEDEHVRKVYLGKNFELRR"
     gene            complement(14502..>14961)
                     /locus_tag="PLZ75_03500"
     CDS             complement(14502..>14961)
                     /locus_tag="PLZ75_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOS71497.1"
                     /translation="VFSTTMLFFSGVLFGYFLLAPLSIHFLGSYHVSEDVVNQINIRS
                     YIGTISSICLATGVIFELPVIAFFLTKIGVLTPAFMRQYRKHSIVVIFILAAIITPPD
                     VFSQMLVAIPLLILYEVSIGISGRVIRKKEKEHDAFMMEDSIEKAQNTAT"
BASE COUNT         3818 a         3391 c         3512 g         4240 t
ORIGIN      
        1 caataaagat acggaaacaa gatacaaggc taaaaagaaa gcagttgtat cgaaaaataa
       61 aaaagccggg agaaagtacc gttaaaacca caaatctgaa tctgtaattc tctaattttc
      121 agagtaattt ttttttgttt ttttaaaaaa aattgtatat ttgtctaatg aagagttccg
      181 gaaaccccgg aattcttctt atttttttaa ttctttacag taagggaaat gtcaaaggtt
      241 ttgcatgtta cagaaggagg ccttcagaaa ttaagggaag agcttgacca tctggtcgca
      301 gttgaaaggc cgttgatatc caggatgctg tctgatgcga gggataaggg agatcttgca
      361 gagaacgctg aatatgcagc agcgcaggaa gctcaggcca tgctggaaat gaagattgcc
      421 cggctggagg atataatagc caggtccaga gtgattgatg aatcacgaat tgataattca
      481 actgtaagga ttttacacaa ggtcaggatc aggaataaaa agaataactc cgaaatggaa
      541 taccagattg tccctgaaag cgaagctgat ctgaagaggg gtaaaattgc ggttaattcc
      601 cctatagccc agggacttat cggaagaaaa aatggcgaaa ccgtagagat aaaagtgcct
      661 tccggaataa tggtttttga aattgtgtca atatcattgt gatcaaataa gctgaagcat
      721 ggcaactata ttttccagga tcataagggg tgagataccc tgctacaaga tcgtcgagga
      781 tgacagctac tttgcatttc ttgacatcaa tccgctgaag gcaggtcata cccttgttat
      841 cccgaagcag gaaacggatt atatttttga tctcgatgat gaaatgcttg ccggactgat
      901 tcttttctcc aaaaagattg ctgttgccct gaagaaagca ttgccctgta acaggatagg
      961 cgtagcggtg ataggtctgg aagtccctca tacccacatt catcttgtgc cgatggattc
     1021 aatggaagat ttgaatttcc ggaatcccaa gctgaaattt tcacaggatg aattcaatgc
     1081 tattgcagaa aaaatccgca gcgaaataga tttttaatac ggccgtttat tcattatttt
     1141 acaggataac tcatttcgtt tgcagaaaaa atccgcagcg aaataggttt taattacagc
     1201 aatttattct taattttaaa ggataattca tttcgctgca tgaattacct gcatgattta
     1261 tgggacgatc tgatctcagt tcttttcccg aggctgtgcc aggcctgcgg aaatcacctt
     1321 atgcggaacg agaagctgat ctgcacggaa tgtcatgttc ttattcccag aacggatttc
     1381 catcttaccg atgacaatcc gcttgccagg cttttctggg gaagatgcag aatggaaaag
     1441 gcggcagcct tctctttcta caacagggac agccggatca ggaagcttat tcataatctg
     1501 aaatacaagg gtgtacagga gatcggaatt gaactgggca ggatttacgg cctgatattg
     1561 ttcagttcgg gttttacagc cgatatggat attattgtgc cggttccgct tcacccggac
     1621 aaggagcgtt cacgcggctt caatcagagc cacctgatag cccggggcat ctccgaagta
     1681 tcgggtctgg ccctcgacgc ggcttccctg caaagggtgc ttacatcatc cacacagaca
     1741 aagaagtcaa ggtttgaacg ctggaggaat gttgaggatg cattcctcat cagtaatcct
     1801 gctcctttcc gggggaaaca tgttcttctg gtggatgatg tgataacaac cggagccaca
     1861 atagaatcgt gcgtgaatga actgatcggg tcgggtgcgc tgaaggtgag tgctgcggca
     1921 ctggctgccg cactgctgta aaagaatggc tttccctggg agatgtggct attttattct
     1981 tccgggattt tccctgatga actgtttcca gctttgggct ttgctgccgg ccgctgatgg
     2041 cctgccggtc tggtaaaaat ggcaaagggc cgcagccagg ccgtcggttg catcaagctt
     2101 cagctctgaa tcgcggaatc tcaggatttt catcagcatt gccgcaacct gttccttgga
     2161 agcggatccg acaccggtaa ttgacaattt gatctttctg ggtgcatatt cgaatatggg
     2221 gagtgaacgc gacaatgctg ccgctattgc tgccccctgg gctcttccga gtttaagcat
     2281 cgactggaca ttcttcccgt aaaagggagc ttcaatcgcc agttcatcag gatgatactc
     2341 atcaattatt ccggtggtgc ggtcaaagat atgtttcagt ttcagatagt ggtccccgaa
     2401 ctttgaaagg tctatacatc ctatggttat cagttcgggt ccggcaatgc ccgcccttat
     2461 cacaccgtat ccggtaattg tggttccggg gtcaatacca agaattattc tttctgccgc
     2521 tttctgtttc actgccatct gctttgcttt ttcccggata aagacattat tcatccgcag
     2581 aagtttcctc aataccggat ctgccgtgtg gatgcctttg cttcactgat ccttaatccg
     2641 gatcaaagat atgaaattat atccctgatg ccttatgatt ctgataccat attcctgata
     2701 ttatgaacag taaacctgcc ggagttgtgt agtaaagggg ttcatgcagc aggaaatgaa
     2761 tgaataccag ggaaaggaag ggggcaaggt aaaccaggtt gcttattcct gctgttgttt
     2821 ctgccgtttt cagtgccctg agccagagat aaaaagtgat acccatttca aacaggccaa
     2881 tgtaaatggc cgaaagaatg tctttcagtc cgtatttgcc ggtaagggga agttccgtga
     2941 cgatgaaaag gataataaga taaagcgatc caaaggcaaa attcagaaag agctttacgg
     3001 cttcctctct tttgtctctt acgttaagga tgaaataaag tgcccagaat acagagctgc
     3061 ccgttgcgag caatacacct ttaaggtctg atctgccggg atgcaggaga tctcccctgg
     3121 aagaaatgat atatactcct gcaaagctga tcaataatgc aatgtaactt ttcctgctta
     3181 ttttctgtcc gagaagggga acagacagga agaccaggac aatcggccag atcatgttca
     3241 gcggctgggc cacctgtgcc ggaagcagac tgtatgcttt cagcaggatc agataataaa
     3301 cgaatgggtt aatgaatccg agaagggcgg aattaaaaag ctctttccgg cctgatgccg
     3361 cgataagccc ggttttcttt tcgcctgcca gtattgcaag cagggcaagc gcggatgtga
     3421 cagatgccag ggtaagcatc ggcactattc ccagttcacc caggcagatc ttgaatgcgg
     3481 tgggaatggt tgaccagaaa aatactgcaa gaagggcaag catgtatgac tgccgggatc
     3541 tgttcatttt taaccacgat taaaaatgtg ttcctgggat tgccgtgatc cccgtttcag
     3601 tcaatcattt caaatcctgt atacggaatc agtgcctgtg gtattctgat gcctgagggg
     3661 gtctggttgt tttcgaggat ggcagcaacg attcttggaa gagcaagtgc gctgccgttc
     3721 agcgtatggg ccagtctggt ctgttttccg cctttttccc tgaaacggca tttcagcctg
     3781 tttgcctgga atgattcgaa gttggatact gaactgactt ccagccatct ttcctgggcg
     3841 gctgaccaga cctcgaaatc gtaggtgagt gctgaagtga atgacatatc gcttccgcaa
     3901 agcctcatta tccggtatgg aagcccgagc tttttcacca ggccttcaac atggtttacc
     3961 atttcatcaa gggccttgta ggaatgatcg ggatgcgcta tctggacaat ttcaaccttg
     4021 tcaaactgat gaagcctgtt cagtcccctt acatgggctc cccatgagcc ggcttcgcgc
     4081 ctgaaacagg gtgtgtaggc tatatttttt acgggcaggt cttctgcatt cagtatcaca
     4141 tccctgtaaa tatttgttac cggaacctcg gctgtgggga tgagataaaa attatcaagt
     4201 gtcatatggt acatctgtcc ctccatatcg ggcagctggc ccgtcccgaa acctgaatcc
     4261 tcattcacca ctatcggtgg tatcacttcg gtatagccgg cactgacacc ttcgtcaaga
     4321 aagaatgaaa taagggatct ttcaagcttt gcgccctttc ccttgtatac ggggaaacct
     4381 gcaccggtca gcttgtttcc cagatcaaaa tcgatgatgt catattttct gatgagttcc
     4441 cagtgcggaa gcgcatctgc gggaacagaa ggcttttcac caccttccct gaccttcaca
     4501 ttatcttcgg aactgctgcc tctgacaacg gattcatgcg gcagattcgg caagcggatt
     4561 atttcctccc ttaattccga atcgagcctt ccaagctctt cacccagctc tctgatttcc
     4621 tccttcagcc ggtaggtgtt ttcctttgct gattcagctt cagttttcct gttctgcatc
     4681 attagtgaac ctatctcctt tgagatcctg ttcatttctg cctgcattgt atcggatctc
     4741 gactggattt cacgccggcg ggagtcgagt tccaggattt tatccagaat ctcttccgaa
     4801 ttgaaatttt ttattttcag acgatcggaa atgaaatctc ttttttcgcg gataagattg
     4861 atagtaagca ttttgtatga gatgttgtaa aaataaaaaa ctcccggcaa tttttccgtg
     4921 gccgggaaag atagcgggag tcaggattct tcagaaagtg tgcttatatg aacaggtttg
     4981 cccagattct taattctgct gttcagtgga agctttttct gcaactgttt ctttctcact
     5041 tattacagaa acgaaaacct tatcgttttt ccttttcctg aattcaataa cgccgtcggt
     5101 gagggcaaac agggtatggt ccttaccctg gccgacattc tgcccgggat taagccttgt
     5161 tcctctctgg cggacaataa tattgcccgc tgtcaccttc tggccgccaa agagtttcac
     5221 tccaagccgc cggcctgttg aatcacggcc gtttcgtgaa ctgcctgctc ctttcttgtg
     5281 tgccattcta ttttaatgtt ttaatatttc tgttattact cattcgcctt tgcggaatcc
     5341 tgttcctcag ccctgcttcc ttaagctgtt tccgtttcgt ctgatcctgt ttccgcggaa
     5401 tccttttcct ttgccggagc tgttttcccg gtttttttaa cggattcttt ttcagtttct
     5461 gctttcaggg gagcatctgt cttcccggct ttttttgcac tgctcttttt tgcagctgcc
     5521 ggttttgctg aggcatccgt cctggcggca ggtattgcct ctgcgccggc ttcagctgcg
     5581 gcagcttcca cggtaactgc cttttcagct tcagcttctt ttcctgaacc tgcttttttt
     5641 ccgccgatat ataatatttc aatctgggta aaatcctggc gatgaccgtt tttaaccctg
     5701 tagccctttc ttctcttctt cttgaaaaca atcaccttgt cacccctgac ctggtcgttc
     5761 aggactttcc cctcaaccac gaaatccctg atgaagggtt cgcctattat tatttttcca
     5821 tcatcctcaa ccagaaggac tttgtcgaat tctattcttt ccccttcttt ctcttcgagc
     5881 cggtggacat atattttgcg gccttcttca actttaaact gctgaccagc aatttctact
     5941 attgcgtaca ttctgctacg ttttatgttt ctccgccagg cgtatatcct acagggaata
     6001 tagctttgac aggcctttac ggattatctg aatttggact gcaaagatat acaaaatagg
     6061 caatttccaa actataaagt attatttttg caggctgatg aaacctttat ttatgcaatt
     6121 ataatcctta tatttgtctg aggctaaaca aaagaaagga gaaagatatc agtgatgaag
     6181 agggttaagc tgaaagttat gggaatttct tacagtcaga cacaatcggg cgcatatgcc
     6241 cttttactga ttgaagaaaa cggagagaga agaattccga taatcatagg aggatttgaa
     6301 gcacaggcca tagttattaa acttgaaaac cttgaacctc ccaggccatt gacacatgat
     6361 ctgttcaaaa agttcgccga ccgtttcaac atagctgtaa ttgaagttat gatctacaag
     6421 ctggaggacg gggtgttctt ttcaaaactt ctatgcaaca acggggagaa ggaatattca
     6481 attgacagca ggacatctga tgcggttgcc atagcactga gattcggatg ccccatatat
     6541 atcagcgagg acatactgga aaaggcacag atctcaaatg caccttccga aaccgatact
     6601 gtcgtttccg gtgaatccgg atcaaaacgg aagccatcat caaagtacga ttcattctcc
     6661 gacgaggagc tctataaaat gatagatgag gcaataaaga cggaggatta cgaaaaggca
     6721 gcctctatta gggatgagat tgaaaaaagg aagggaaaga aaggctgaaa ggacaggaaa
     6781 tcactgttcc gcctgcagat tgtgcccgtg ataaaatacc ggacagatac atgattttta
     6841 ataacccagg gaaaatgaaa caattattcg tattcttgtt tgttctggcc gggataaacg
     6901 cccatccggc tccggcagtg tacaaaataa gttctcctga cggaaagatt gccgtggaac
     6961 tgagtgcagg cccggatatc cgctggtctg caacttatca gaacagtgaa ataataagtt
     7021 cggtgaaagc cggactggtt agcggagacg gcaggttatc cggagagaat gaatccgtcc
     7081 gcagggcaca gtacgggaaa atcgatgatg ttcttgaacc ggttgttgct cacaggcatt
     7141 caagaataga agacaaatgc aacacgctca ggatttcttt caggtcgggc ctggcccttg
     7201 atttcagggt ttacaatgac ggaatagcgt acaggtttga aacggcactg aagggtgagc
     7261 tgattgtcag ggatgagatt tcggaactga agtttccctc cgggtctttt tcatattatc
     7321 cgcttgagga gagattcatg tcacacaatg aaaggaaata tatttattcc tcactggata
     7381 ccctgacaga catacatctt gcaagccttc ccgtactttt caccacaaag ggaataaata
     7441 ttctgcttac agaggccgat attcaggatt atccgggaat gtggctcagg ggtatggggt
     7501 ccggcggact gagggctgtc tggccgccct gtcccgaaac ggaagaactg agaggcgacc
     7561 gggatgtttt tgttacaggt gcaagggatt atattgcctc gaccgcagga agcaggactt
     7621 tcccctggag ggcattcgtg attgcggaaa atgatggcag actcattgaa agcgagctgg
     7681 tatacaagct cgccgccccc ctgaggctga aagacaccgg atggataaaa cccggcaagg
     7741 ttgcctggga ctggtggaat gcgaacaata tttacggagt ggacttcagg gcagggataa
     7801 acactgaaac ctacaggtat tatattgatt ttgccgcggc aaacgggatt gaatacataa
     7861 taattgacga cggatggtat cccctggggg aaacagtatt gaaatccgta ccggggatgg
     7921 atgttcctga actgtgcaga tatgccgctg aaaggaatgt gggagttatt ttgtgggttg
     7981 catggaacct gttctgggat gagattgatg aggctgtggc actttatgaa aaatggggtg
     8041 tgaagggtgt aacggtcgat ttcatgcaga gggatgacca gaaaattgtc aacttctatc
     8101 atgaagctgc aagaaagacg gctgaacacc gcctgctgat caattttcac ggatcgtata
     8161 aacctgacgg gatgatccgg acatggccca atgccctcac cagggaagga gtgatgggtc
     8221 tggagaacaa taaatggagc agtgatatca ctccctctca tgaactgacc attcccttca
     8281 tcagaatggt tgccggacca atggatttta ctcccggggc gatgataaac atgggacagt
     8341 cagagtttca tccgctgttc aaccgccctt caagtcaggg taccagggct caccagatgg
     8401 ccctgtacgt tctttatgaa agccccatgc agatgctttg cgattcccct gtcaactata
     8461 tgagggagca ggaatgtact gattttatcg tgaagagccc ggtggtatgg gatgacctgc
     8521 ttgtgctgga tgcaaaaatt acggaatacc tgctgctggc caggcgctcg ggaaatgact
     8581 ggtttgcagg ggcaattact gactggacag ccagggatct ggagcttgat ctctcctttc
     8641 tgcctgaagg cgagtaccgg atggaaatat tttgtgacgg catcaatgcc gaccgtcatg
     8701 cacaggatta caggcatctc acaaaaaccg tcagacaggg ggagaagctg aaaataaacc
     8761 ttgtatccgg aggcggatgg cttgcaaaaa tatcaccaat aaagtaaatt ctgtaaaaca
     8821 agatgaaaca gtatcttgag cttcttgagc atgtactggc tgacggagtg gaaaaacagg
     8881 acagaaccgg tacaggcacc ctgagtgttt tcggatatca gatgagattc agcctgcagg
     8941 aaggatttcc tttgctgaca accaagaaac tccatctcag atcaattatt catgaactgc
     9001 tgtggttcat ctcagggaat acaaatatcc gctaccttca ggataacgga gttaagatat
     9061 ggaatgaatg ggccgacggg gaaggcaatc tgggaccggt atacggctat cagtggaggt
     9121 catggccggc tgctgacggg agaaggatcg accagcttgc cggagtgata gaaaacataa
     9181 aaaacaatcc cgattcaaga cgccttattg tgagtgcctg gaatgccggt gaacttgaca
     9241 ggatggcgct gcctccctgc catctcctgt tccagttcta tgtttcagcc gggaaattgt
     9301 catgccagct ctatcagcgc agttgcgaca tttttctcgg agttcccttc aatattgcat
     9361 catattcact tctgaccctg atgatagcac aggtaaccgg cctgaagccc ggtgaattca
     9421 ttcacactct gggggatgca catatctacc tgaatcatct tgagcaggtg aagcttcagc
     9481 ttgaacggga accctttccc ctgcctgaaa tggaaatcaa tcccgatgta aaggatatag
     9541 attcattccg ctatgaggat tttacactta agaattatat atcacatccg cttatcaggg
     9601 gagagatttc cgtataaata ttttctgtat gatctcaata attgccgctg tatccgaaga
     9661 tctcggcctg ggcagaaaca atgacctgct ctggtatatt cccgaagacc ttaaaaggtt
     9721 taaacggctc acctacggga aaaccgtggt aatgggcaaa cggacctggg aatcattgcc
     9781 cctgaagcct cttcccggac ggaagaatat tgtaataagc gacattccgg gcgacagctt
     9841 tgagaatgcc ctgactgcct gttcgataga agatgcaatg gataaatgcg ggcatgacgg
     9901 ggaaatattc attatcgggg gagcaagcat ataccggcag ttcatgcctt atgccggcag
     9961 gctgtatatc acccatgttc acatgaacaa gccggctgat gtctttttcc cggaaataga
    10021 catgagcagg tggaaggttg ttgagaggga ggaataccgt gacacagggc aggaaagcat
    10081 tccttacagc tatattgtat acgagagaaa ggagccgggt tcataacggg ttttcccggg
    10141 aacccggtat tattaagcgg gaaagtatct tatgctatga aactctcaaa accttcatgt
    10201 cgattttgga aagcctgtgg gccgcatagt cgactgtgat caggaggtca tccctgccca
    10261 gggaaggcat ttcgtacatt gcatctatca ttatcgtttc gcagatcgat ctcagccctc
    10321 ttgcaccaag tttgaactca actgccttat cgacaataaa atccagggcg tcgtcggcaa
    10381 aggaaagttc gatgtcatcc attttgaaga gcttgatata ctgctttaca atcgcattct
    10441 ttggttctgt aagtattgcc ttcagtgctt tcctgtcgag gggattgaga tgggtaatga
    10501 cgggcagccg gccgattatt tccggtatca gtccgaaagc cctcagatcc tgtggcgcca
    10561 ggtatttcag aagattgtcc aggtcgacct ttgatctgac cttcgatgcc ccgaatccca
    10621 ctatctgggt attgagcctt gctgctatct ttttttcgat tccgacaaat gcacctccgc
    10681 agataaagag tatgtttttt gtgtcaacgg gtatcatttt ctgctcggga tgctttcttc
    10741 cgccctgggg aggaacattt acaaccgagc cttcaagaag tttgagaagt ccctgctgta
    10801 ctccctcgcc cgacacgtca cgtgttattg agggattgtc accttctttc ctggcaatct
    10861 tgtcgatctc atcaatgaat atgattccct tttcagctgc cttgacgtca tagtcggcat
    10921 tctgaagcag ccgggtgaga aggctttcta tatcctcccc gacatatcct gcttcggtaa
    10981 ggactgtcgc atcaacaatg gcaaagggaa catgcagcat ctttgcaata gtctttgcca
    11041 gaagggtttt gccggttccc gtttccccga caaggattat gttggatttt tcaatttcaa
    11101 cgtcatcatc atccgtcttc tgtgtaagcc ttttgtaatg attgtataca gcaaccgaga
    11161 tgattttctt ggccatttcc tggccgataa catactggtc gaggaatttt tttatttccg
    11221 gcggcttgag aagatttatg gtatcgaacc tcgtgctggc ctttgtcccg agctcgtcgg
    11281 tcattatgcc gtgggcctgc tcgatgcaat gatcgcagat atgcccttca aggccggcaa
    11341 taagtatgtt ggtttccttt ttcgatctgc cgcagaatga acacttgctc atgtcttttc
    11401 aatgtacggt tttctgaagg atttcgtcga tcattccgta ttcctttgct tcaaaggatg
    11461 tcatccagtg atccctgtcg gcatcctttt caactttctc cagcggattg cctgtatgaa
    11521 gcgcaatgat ctcgtaaagt tcctttttta ttttcattat ttcacgggcc tggatctcga
    11581 tatcggatgc ctgcccctga aagcctccca tgggctgatg tatcatcagc cgtgaatgct
    11641 tgagagccga tctcttgcct tttgttcctg cagtgaggag aacagctccc attgaagctg
    11701 ccattccggt gcagatggtt gctatgtcag cggatatgta ctgcatggtg tcataaatgc
    11761 cgagaccggc atgtacgctg cctcccgggg tgttgaaata aatctgtatg tctttccccg
    11821 ggtcggagga atcaagatac aggagctggg cctgaatgat atttgcaaca tagtcatcaa
    11881 tggggagtcc caggaatatt atcctgtcca tcatcaggcg tgaaaaaaca tccatggatg
    11941 ccacgttcag ctgtctctcc tcaatgatgg taggggaaat ataattgttc agaaccgagg
    12001 tatacctgtg aagggtgaga ctgctcagtc cgtatctgct gcctgcatat tttctgaaat
    12061 cgtcgctgtt gttcattatt gtattttatt gtttgatact attgaatctg atctcttatt
    12121 gccggatctt tattttattt attcactgcc gcagttacaa agttacaaag attaatcctg
    12181 aattttccgc gtgattcaat tttcaaaaag tttgttaaac tcttcagcag tgaccttttt
    12241 gttttcaatc tcaacaactt ccttcagtgt gttcagtact ttatcatcca gaattttatc
    12301 ggcaatccgc tttgcgtccg cttctctttg aagggtttcc cttgcatagc cggatatctg
    12361 ttcatcagtg gcatagtaaa gtccgtactg agaaaactgg aacctggtga ttttttcagc
    12421 ttcttcctgc atttcctcgg gacttatctg tatttcattg tcctttgcca ctttattttt
    12481 gatcagctgc catttaagat cctggatgaa tgtgccgaat tcattttcaa tctcttcaga
    12541 tgaagttttt tccccggcct tcatcagcca ttttttaaga aattcttcag ggaggcggaa
    12601 ttcagttttg tcgagtgtga gcttcctgac atcctgcatc agtttaaact cactttcctt
    12661 ccttaggttc gcggatattt cctcttcaat cttctgcatg aattcctcct cgtccgacac
    12721 caccccctca ccgtaaattc tgttatagag gtcaggaccc gtttctgccg gataaaacct
    12781 tgatatctct tttacggtaa gccggaaatc tccttcaata ttttccgcat tcctgctttc
    12841 cattctgagc agaccggcta tttcagccgt gtttggaaag gcctttctta tactgaaaac
    12901 aacactgtca ttctcagcaa gcccgatgaa tgattttttg atttcctcat cccttaccat
    12961 gtcaaccgac aggctcgtct cgtcggcaga aataccctca gggatgactg cgccattttc
    13021 gtcaacagct tcaatcctgc ccttcagaat atctttttca ccgcttgctt ccgccttcct
    13081 gaattcaccg aaacggcgtg catagctgtc ggcataatcc ttcttcatct tgtcgtcgat
    13141 aatgatttcg tggaaggtga ccctgtcgcc cttgccgaga gtcagttcga aagatggtgc
    13201 gagcccgagt tcaaaggaaa aggtgaactc atcgtcatgt atgaaatcgg ttttttcatt
    13261 ttcatctgtc agcggaagag gatccccgag gatgtcaatc ttttcatcaa caaggtattt
    13321 atgaacattt tccgaaatca gcttatttat ttcatccacc ttcacggcat tcccgtacat
    13381 gcttcgtata acgccgatag gaaccatgcc gggcctgaac ccttttatgt ttgcctttct
    13441 ccggtaatcc ttcaggactg tttctactct ttcatcatag tctgacttgc tgatcttcac
    13501 tgtcagaaca gcattcagat cgtctttgtt ttccctggaa atatccataa caaaatatta
    13561 ttgtattaat cagaaatcat tggcggcaat attgttgcac aaggaatatg aagcgcctcc
    13621 gacattctgg tgcggatgaa gggactcgaa cccccacgtc attaagacac aagatcctaa
    13681 gtcttgctcg gctaccaatt acgacacatc cgcagaaaaa tctccgcaaa attactacaa
    13741 ataaaattaa ttgcaataca gaacggctta tcgccgcagt tcgaagttct ttccgagata
    13801 tacctttcta acatgctcat cttctgccag ctgtgatgca gtacccgatt taagtatttt
    13861 tccctcgaag aggaggtagg ccctgtcggt tatcgacagg gtttcgtgga cgttgtggtc
    13921 ggtgataagt atacctatat ttttgttctt gaggtgggag acaatattct ggatatcctc
    13981 caccgcaatg ggatctatgc cggcaaaggg ttcgtcaagg aggatgaact tgggcttgag
    14041 ggcgagggcc cttgcaattt cggtacgtct tctctcgcct ccggaaagct gaataccctt
    14101 gctctttcgg attttctgca gaccgaattc gtccagcagg gattcaagcc tttgtgcctg
    14161 ttcccttcgt gaaaatgccg acatttcaag aacggcccgg agattgtctt caaccgaaag
    14221 attcctgaaa acggatgctt cctgggccag atagcccaga cccttccttg cacgcttgta
    14281 aacgggaagg tcggtgatat cctcgccgtc aaggaatatt tttccttccc tcggacggat
    14341 caggcctact atcatgtaga atgatgtggt ctttcctgct ccgttgggac caagcagacc
    14401 cacaatctcc ccctgtgcaa cctcaaagga aacatggtca acaaccgtgc ggttatgata
    14461 tttttttacc aggttttcag tatgcagttt catgacatgg atcaggttgc cgtattttgt
    14521 gccttttcaa tactgtcctc catcatgaag gcgtcatgtt ccttctcctt tttcctgata
    14581 actcttccgg agatgccgat acttacttca taaaggatca ggagcggaat ggcgaccagc
    14641 atctgggaaa acacatcggg aggtgtgata atggcggcaa ggatgaaaat caccacaatg
    14701 gaatgttttc tgtactgtct catgaatgcc ggtgtgagaa ccccgatctt tgtaaggaaa
    14761 aaggcaatta ccggaagctc gaatatcact cccgttgcca ggcagattga cgagatggtg
    14821 cctatatatg atctgatatt gatctggttc acgacatcct cgctcacatg gtaggaaccc
    14881 aggaagtgga tcgaaagggg ggcaaggaga aaatagccga aaaggacacc ggaaaagaaa
    14941 agcatggtgg tggaaaatac g
//
LOCUS       DAOSPX010000040        14743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4159_length_14743_cov_4.310934, whole genome shotgun sequence.
ACCESSION   DAOSPX010000040 DAOSPX010000000
VERSION     DAOSPX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4159_length_14743_cov_4.310934"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /gene="pcm"
                     /locus_tag="PLZ75_03505"
     CDS             <1..240
                     /gene="pcm"
                     /locus_tag="PLZ75_03505"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="catalyzes the methyl esterification of
                     L-isoaspartyl residues that are formed in damaged
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOS71498.1"
                     /translation="YGDGYQGWPEHAPFDIIVVTAAADQIPQPLIDQLAENGRLVIPV
                     GPPAAIQELILITKKNGKPVERRLTYVRFVPFKRL"
     gene            complement(288..364)
                     /locus_tag="PLZ75_03510"
     tRNA            complement(288..364)
                     /locus_tag="PLZ75_03510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(328..330),aa:Arg,seq:tct)
     gene            969..1685
                     /locus_tag="PLZ75_03515"
     CDS             969..1685
                     /locus_tag="PLZ75_03515"
                     /inference="COORDINATES: protein motif:HMM:NF016209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HOS71499.1"
                     /translation="MKTYIRRREKNDSLVVMYGAWGTDENVFEPLCNDEFDFILFYNY
                     SADQALVLPEMKTYKKITLIGWSIGVWAAEYLSPSIGIMPDVTIAVNGTPVPLDDNYG
                     IPPRSFEEALDDITEESIEKFYYRMFGNRRTFNLNSDRIPGRTLDSFHVELRWLYNRV
                     MEQREPGFRWDFAVTSKSDKVFPTENVTKYWDKMGNTRQIIVPFPHYMFYKWPSFNEF
                     INYVEKRGRIKSVSPETTGL"
     gene            complement(1686..3032)
                     /locus_tag="PLZ75_03520"
     CDS             complement(1686..3032)
                     /locus_tag="PLZ75_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOS71500.1"
                     /translation="MANPELSWPFKKIKQWNDAIWHTPLSELSRRKSFLIRQLRIIVL
                     AAQNSLKDKVSLRASALTFYTLISIIPIVAIGLAIAKGFGLDQNLEAIIRNTKEFTLY
                     NDILNPLLENAKNTVQRTRGGYLAGIGAVILIWSVVSLLGQIETSFNHIWQTSVSRPW
                     HRKFTDYLTILLIAPIFIILSSSISIFIITSLSEYMDRVPILASFKWLVSFLIKSLPY
                     LLTWLMLTLLFIVMPNVKVRFGPALISGIIAGTFLKILQWLYLDLQFNITKLSAIYGG
                     LAAIPLFLVFLETCWIVILLGAELSFANQNVSRYEFEAEALNISHYQKRTIILLVMYL
                     IIRSFYLGEKPLTSEEISRELKIPVRLARDILEDLEETGLVSVIHEHENKERSYQPAM
                     DINKLTVSFVLSRVDKRATQEKYFMRSREYEKVTQILDSFDALASGSESNILIRDL"
     gene            complement(3120..4061)
                     /locus_tag="PLZ75_03525"
     CDS             complement(3120..4061)
                     /locus_tag="PLZ75_03525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS71501.1"
                     /translation="MTISLLIPVCDYDIVALVHSMKSCIGKVPELIEIIIGDDGSSPE
                     YKKKYKLLKGDGVEILESPVNIGRAAIRNRLALAAKGDYVLFIDADTMMPGTAEDYIR
                     KWIPAMNVSRVTCGGVIYHKSPPGDPDKILRWKYGRKNDGIRASERNKHPYHKFSTFN
                     VLFDRTIFSKLRFNEDLKQYGHEDTLMSYQLRKAGISILHIDNGLIHEGLESNREFLN
                     KTKLGIENLSKLYDIVTDKMTFSSTVRVLQIYSILKWFRLTLILAGIYIRYRERMELR
                     IDSANPSLWLFRFYKVCLFCTYREIHGRRKILPSLNI"
     gene            4188..5606
                     /locus_tag="PLZ75_03530"
     CDS             4188..5606
                     /locus_tag="PLZ75_03530"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOS71502.1"
                     /translation="MRKFLQITVLLFLSIHTYSQEKAGYEISVTISDISDSTIFLAYH
                     YGDRQYLSDTLKLDGKGQGIFKGKEKLPEGIYMIVLPGRKYFEFLMTDNQHFSLSCLY
                     NDFFRTLKFSGSPLNTAFTDYQRQWSQMQQKASGIANRLQQNKANSDSLRILSGKQRE
                     YESEMKAYLRTVAGKNSSNFLGVLVKSMIPLEIPEFSIPAGTNNPDSVRWYLRYNYNK
                     DHFFDNIDLTDERLLRTPILQARLEVFFKNVIIQSPDSINKEIDRIFPKTGNNPKMFQ
                     FLSVFLFNHFRQSEIMGHDAVLVKLADDIYLSGKADWITEEFRNDLHRQVELLRHNLL
                     GMTAQNLVMDSYKGIFVALHDIEKEFTVLYFWEPNCGHCNEATPKLKAWYEKAKNDGV
                     EIFSVCTTSNRQEWADYIEKHGLTWINGWDPNRQTHFDYYYNVQATPLIYILDKNKKI
                     IAKKLSVDDIPSFIDNYRKYFR"
     gene            complement(5599..6618)
                     /locus_tag="PLZ75_03535"
     CDS             complement(5599..6618)
                     /locus_tag="PLZ75_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS71503.1"
                     /translation="MKQQTEEILTVNSLIIGYRYGRKKIPVLPPLGASAGKGELTAVL
                     GRNGIGKSTLLRTITGLQKNIGGSVLIRDEDTSHYSRLKFARTVGYISTEIVKVSHMR
                     VYDLVALGRFPYTDWTGRIDRTADEIINDSIKKAGLAGFSDRIISELSDGERQRAMIA
                     RALAQNTDILIMDEPLAFLDISGKFEVLRLMKELTASGKTIVFSTHDFNIAVTNADKI
                     WLITDKGIIEGAPEDLWLTNNYECLFDYEHTGFNPEDGSFSFSTACTSDIYLEGSEGK
                     KKNWTVKALRRNGFRISADKTQPFVRMLEGEEGWSLVHGDEVRNSGSLYDLLKLLRQY
                     NNVPT"
     gene            complement(6618..7697)
                     /locus_tag="PLZ75_03540"
     CDS             complement(6618..7697)
                     /locus_tag="PLZ75_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOS71504.1"
                     /translation="MTDGKNITGHISHTFSPSVLPVQKLIFTGFIVLLLILFGLDLFL
                     GSVSIRPADVIRSLTGTGDETVQTLILKFRLPKAITAVLAGIALSLSGLQMQTVFRNP
                     MAGPYVLGVSSGAGLGVALVILGFSSGMGPASVTAGGSWLLVSAAWTGAGAVMILMLF
                     ISARVRSIMTVLIIGMMLASGISAIVSILQYFSDESMLKAYVVWTMGSLGNLSSEQLK
                     VLAVSVAAGVILSMASVKMLNALLIGENYASSAGLNVTAARIIIFAGTSILSGSVTAF
                     CGPIAFIGIAVPHIVRNMLRTSDHRLLVPGTVFAGATVMLISDILSQLPGSEKILPVN
                     SVTSLIGIPVVIWIILRKNISSELF"
     gene            complement(7694..8848)
                     /locus_tag="PLZ75_03545"
     CDS             complement(7694..8848)
                     /locus_tag="PLZ75_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS71505.1"
                     /translation="MQRYKLLYLFLSVSFLLMIFSSCSRDRKREKIQKNTGGNISHAE
                     RFDLHATDSCTILRITDPWQGAGNVIHEYYLVRDTGRMEIKAGTSKIISVPVRRIICM
                     STTHIAMIRALGCQNSIVGVSGAGFVFDSDISNRISRGLVSDIGYDSGLNTELIVKSS
                     PDLVMIYGIGSESAGYTAKFAELGLNVMYNADYLETDPLGKAEWIKVFGALYCCEDLA
                     DSLFREIRDSYLEIRELMAGKVPKPVIMLGMPFRDTWYVSPGNSYISKLIDDAGGRYL
                     WKETESAQSMPYSLETVYAAAMKADIWLNTGSASSKAEITALDKRLGSMPPFINNRIY
                     NNNKRLNLTGGNDYWESGAINPHLVLRDIASIIHPEIFSGYEMVYYRKIE"
     regulatory      complement(9039..9253)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9359..10393)
                     /locus_tag="PLZ75_03550"
     CDS             complement(9359..10393)
                     /locus_tag="PLZ75_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOS71506.1"
                     /translation="MKKFKNKDLAARLGVSGTLVSLVLNNKADQHGIRKETQEKVWAM
                     ARQMGYFSSHEEKGQITPHDEKPGIIGMIVPSSGNSFVQSITPYLQKSFHSIGLGFSI
                     ITKDPDDQRYDRMIGAFRKFFSGLILMGETADDQTIRTLRTASCPFILLEKSIKTGRL
                     STVNTDTAAGIQLVADHVNKLGYKNIIIATDKKSAKHDTDAINQLISALTEKGRQVNK
                     PVVVEFEASLTDNEYYFNQIEQFLRPPFRADIILVMHAELVYPVMSMLRHKKLRVPQD
                     IALISMEDGPGFDLMFTPVTCLKKPVSGLALKAANMIWSEIKNAGKGKFKRQVNLPPS
                     LEMRNSCGTL"
     gene            complement(10542..12512)
                     /gene="yidC"
                     /locus_tag="PLZ75_03555"
     CDS             complement(10542..12512)
                     /gene="yidC"
                     /locus_tag="PLZ75_03555"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOS71507.1"
                     /translation="MDKNTITGLILIFLIFIGFSIYNNSRLNKEFEKALTAAEAEYDK
                     GNFERARTEYVNALRFKPNHPDVLERISELNQKLGTIPGVTEIPADSAVAEPEAVRPT
                     DRSVTPADDPGQYGVFAASALGEDEYITLENDKIDLRISLKGGRVYSARVKDFTTHDD
                     KPLILFSGDSTVFGFNFFTSDNKAVQTNNLYFTPLSENRHYFVESEPLSVVLRLVADD
                     ERYIEYRYTLAPGKNSVDYDITFKNMHGILPENQNNIELDWRMYIPQQEKGRQNEDMY
                     STIRYKYYQDDVDGLRLRSNKEVEKVDITTRLSWVAYVDQFFSSVLITDNYFLSASIT
                     SAKTLTSEKYIRYYTSELGVPLENGPETNLALKFYYGPNHISTLRKEGYDMDKIIFVG
                     KNIVGWINRFIIIPVFNWLERYISSYGIIILILTILIKVILFPLTFKSYQSTAKMKVL
                     KPLVDELAKKYPKQEDAMKKQQATMDLYKRAGVNPMGGCLPMLLQFPILFAMFRFFPV
                     SIELRQESFLWATDLSTYDSILNLPFKIPWYGDHVSLWTLLMTISSIVTTRMSGTTSG
                     QDQPGMKVMMYMMPVMFLFIFNNFSSGLTYYYFLANMITWLQNVLSKRFIDADKVLAT
                     LEENRKKPLPKSKWQQRLEQAAKQRGYKPPKR"
     gene            complement(12668..14725)
                     /locus_tag="PLZ75_03560"
     CDS             complement(12668..14725)
                     /locus_tag="PLZ75_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOS71508.1"
                     /translation="MIIDKDPDDSVRDEQSGAPENAAGTERNRNTEELPEASITDQQK
                     EQPGEEKSADEHSEAELPEGDQTEREGGVPVKRRRKTVQESERDSGIEEYESGIENLD
                     DIELPPVDYSGFSKNDLVETLALLIENRPPSEIRSDVDRIKVYFYKKLKQEAEERKSK
                     FLEGGGTLEDYRSWVDPLEAQVKHLLDKYRQKKTDFNRIQEAEKYENLKKKYEIIDKI
                     KELVNREESINKTFNEFRSLQNEWHSVGVVPQNALKHLWENYHHHVEIFYDYIKINKE
                     LRDLDLKKNLEAKVLLCEKAEELLLEPNPVNAFRILQEYHNHWREIGPVPVESKNEIW
                     ERFREATSQINKRHHEYFENQKDVQRRNLEAKIALCEEVEEINLLEIKNFKDFDEKAE
                     KIMALQKMWRTIGFAPKKQNNKVYQRFRDACDSFFDKKRNFYADNREIQQNNLQLKID
                     LCLQAESLQDSTDWKGTTDALIKLQKSWKEIGPVPRKQSEKLWKRFRKACDHFFDRKA
                     EFFSDLDTSYEDNLKAKLVLIRELEEFEAGTDVQEAFDRLKDIQRRWTEIGFVPFNRK
                     EEITNKYRIALNKEFDRLKIGDEDKNILKFRSRLENLKTNPKASRKIRNERDKFFTKI
                     KQLENDIVLWENNIGFFTKSPNADTMIREVRGKIENAKKMIKILEEKVKMIDQSGIDD
                     YNR"
BASE COUNT         3894 a         3365 c         3066 g         4418 t
ORIGIN      
        1 tacggggatg gttaccaggg gtggccggaa catgcacctt tcgacattat tgttgtgact
       61 gcggcagcag accagatacc ccagcccctg attgaccagc ttgcagaaaa cggccggctg
      121 gttattccgg tggggccgcc ggctgcaatt caggaactta ttctgataac caaaaagaat
      181 ggaaaacctg tggaaagacg gctcacttat gtccggtttg tccctttcaa aagattataa
      241 tctcctttcc tgaattctgc agcaagccgg acaggaaaga ggcaccctgg tgattccggc
      301 gcgattcgaa cgcgcgaccc acagcttaga aggctgttgc tctatccagc tgagctacgg
      361 aacccttttc cgccgcaaaa ttatttaatt tttcgattga agcaagttaa tctgtcactc
      421 aagtttgatt ttcccgtctg tccatctgct gtaatatatt aagcataccc ctgttttcag
      481 agttgctttc tgggaaaaac caggaggcaa acaggtcaat tcgggcaggt aaattattaa
      541 tccggattta tgaaaaccat catcaggaat tctgtcaaaa taatacttca tttagtcaat
      601 attattctgc ccttcgtcat aatgtcattt tttatgcttt gtcaagtcaa ttattatttt
      661 ttaatttgtt accaagctca tgctattggt ataagaggag atgagcaagg gttgggagtg
      721 aaaatcaaca ggattgtatt gcagacattg actttcacat ctggaacctg aggaatagcg
      781 ccagcttacc atggttccat ccttttatca gacagtagac acgggaatta tctcataaat
      841 aaaacccgct ccctgatgtt cacgaccacc cttcctcctc ttattttttt tgtacctgcc
      901 caactgccag tcctgttcag taattgattc ccggaaaata aattatcttt atgtttttct
      961 gttaaaaaat gaaaacctat atacgcagaa gggaaaagaa cgatagtctt gtagtaatgt
     1021 acggtgcatg gggcacggac gagaatgtgt ttgaaccctt atgcaacgat gagtttgatt
     1081 tcattctttt ctacaattat tctgctgatc aggctctggt actgcctgaa atgaaaactt
     1141 ataaaaaaat aacccttata ggatggtcca ttggcgtctg ggctgcggaa tacctgtctc
     1201 cttccatcgg tattatgcct gatgtgacga tagccgtcaa cggtacaccc gttccccttg
     1261 atgacaatta tgggatccct cccagatcat ttgaagaagc cctggacgat atcactgaag
     1321 aaagcattga gaaattctac taccgcatgt ttggaaacag gagaacattt aatctcaatt
     1381 ccgacaggat tcccggcagg acgctggatt cattccatgt ggaattaagg tggctgtaca
     1441 acagggtgat ggaacagagg gagccgggct tccgctggga tttcgcggta acaagcaaat
     1501 cggataaagt atttcccact gaaaatgtta caaaatactg ggataaaatg gggaacacca
     1561 ggcagataat tgtgcctttt cctcattaca tgttctacaa atggccttca ttcaacgaat
     1621 tcataaatta tgtggaaaag cgcggaagaa taaagtctgt ttctccggaa accaccgggc
     1681 tttaatcaca gatcccttat cagaatattt gattccgaac cggaagccag tgcatcaaaa
     1741 gaatcaagta tctgagtcac tttttcatat tccctgctcc tcatgaaata tttttcctga
     1801 gttgcccttt tatccactcg tgacaacaca aaactgacag tcagcttgtt gatatccatg
     1861 gcaggctgat atgacctttc cttgttttca tgttcatgga tcacggatac aagtccggtt
     1921 tcctccagat cttccagtat gtcccttgcc agccggacag gaattttcag ttcccggctt
     1981 atctcttcag aggtcagagg tttttctccc aggtaaaagc tcctgataat gagatacatt
     2041 accaggagga ttattgtacg cttctgataa tggctaatat tcagagcttc cgcttcaaat
     2101 tcatatcttg aaacattctg atttgcaaag gacagctcag cgccgagcag aatcactatc
     2161 cagcaggttt ccaggaaaac caggaacagg ggtattgctg caagtccgcc gtaaatggca
     2221 ctcagcttgg taatgttaaa ctgcaggtca agataaagcc actgaagtat cttaagaaag
     2281 gtcccggcaa taattccgga aatgagtgca ggtccgaagc gcactttcac attgggcatc
     2341 actatgaaca gaagtgtcag catcagccat gtcagcagat aaggaagtga cttgataagg
     2401 aaactgacca accacttgaa tgatgccagt atcggaaccc tgtccatgta ttccgacagg
     2461 cttgtgatta taaagattga gatgctgctc gacagaatta taaatatcgg tgctatcaga
     2521 agaatagtaa gataatccgt aaattttctg tgccagggcc ggctgacgga tgtctgccag
     2581 atatgattga aggatgtttc gatctgacca agcagggata ccactgacca gatcaggata
     2641 acggcaccga ttccggcaag gtatccgccc ctggtacgct gaacggtgtt cttcgcattt
     2701 tcaagcaacg gattcagaat atcattgtaa agagtaaatt cctttgtatt tctgataatc
     2761 gcttccagat tctggtcaag tccgaatccc tttgcaatgg caagccctat ggcgacaata
     2821 gggataattg aaatgagggt gtagaaagtg agtgccgaag cacggagact caccttgtct
     2881 ttaagtgaat tctgtgctgc caggacaata attctcagct gccgtataag gaagcttttt
     2941 cttcgcgata attccgacaa tggtgtatgc cagatagcat cgttccactg ttttattttt
     3001 ttaaaaggcc atgataattc aggatttgcc atcagtcagg aattgattgc agaatatctt
     3061 tataactaag ttaatgatga caaatataca aaatctatgt aaatacttca acaatagagc
     3121 tatatattaa gtgatggcaa tattttcctt ctgccatgaa tttcgcggta tgtacagaaa
     3181 aggcatactt tataaaatct gaacagccac agggaagggt ttgccgaatc tattctcaat
     3241 tccatccttt cccggtatct gatataaatc cctgccagga tcagggtaag gcggaaccat
     3301 ttcaggatgc tgtagatctg caggaccctg actgttgagg agaaggtcat tttatccgtt
     3361 acaatgtcat agagcttgct gagattctcg atacccagtt ttgttttgtt aaggaattcc
     3421 cggttcgatt ccagtccttc atgtataaga ccattatcta tatgcaggat acttattccc
     3481 gctttgcgga gctgatagct cataagtgta tcttcgtggc cgtactgttt gagatcttca
     3541 ttaaacctca gctttgaaaa tattgtacgg tcaaacagca cattgaatgt tgagaattta
     3601 tgatacggat gcttgtttct ttcagaggcc ctgattccgt cattcttcct tccgtatttc
     3661 catctgagaa tcttgtccgg atcacccggc ggggatttat gatagattac cccgccgcac
     3721 gtcacccttg aaacgttcat tgccggtatc catttccgga tatagtcttc ggctgttccg
     3781 ggcatcatgg tatcggcgtc aataaacaga acataatcac cttttgcagc cagggcaagc
     3841 cggtttctga tggcagccct gccgatattc accggtgatt caagaatttc aaccccgtcc
     3901 cctttaagca gtttgtactt tttcttgtat tccggtgaag aaccatcatc gccgatgatt
     3961 atctcaatca gttcgggaac ttttccgata cagctcttca tactatgaac aagtgcaaca
     4021 atatcatagt cgcaaaccgg aatgagcaaa gaaatagtca ttatatccac cctcgaatta
     4081 acctgctaaa tttacaacta atttctgaat tagactgagt tcctgtttgg caaagagatt
     4141 gtattgattt atttttgtgt tcattcaatt gagttatttg ttttcagatg agaaaatttc
     4201 tgcagatcac cgtactactg tttttatcaa tccatacata ttcccaggaa aaagcgggtt
     4261 atgagatcag tgtaactatt tctgacataa gtgattcaac aatatttctg gcataccatt
     4321 acggcgatag gcaatacctg tcggatacct tgaaacttga cgggaaagga cagggaattt
     4381 ttaaaggtaa ggaaaagctg ccggaaggca tctacatgat tgtgctgccg ggcaggaaat
     4441 attttgaatt tttaatgact gacaaccagc atttttccct gtcatgcctg tacaatgatt
     4501 tcttcaggac cctgaaattc tccggctcgc cactcaatac agctttcact gactaccaga
     4561 gacagtggtc acagatgcag caaaaggcat cgggcattgc aaacaggctt cagcagaaca
     4621 aagccaacag tgactccctg cggatactct ccggaaaaca acgggagtac gagtcagaga
     4681 tgaaggcata cctgagaacc gttgccggca aaaacagttc aaacttcctc ggtgtccttg
     4741 taaaatcgat gattcccctg gagattcctg aattcagcat cccggccgga acaaataatc
     4801 ctgattctgt aagatggtat ctcagataca actataacaa ggatcatttt ttcgacaata
     4861 ttgacctgac ggatgaaaga ctgctgagaa cccccatcct tcaggcaagg ctggaggtct
     4921 ttttcaaaaa tgtcattatc cagtctcctg attcaataaa caaggaaata gacaggattt
     4981 ttccaaaaac cggaaataat ccgaagatgt ttcaattcct ctcggttttc ctgttcaatc
     5041 atttcaggca aagtgagatc atgggccatg atgctgttct ggtgaagctg gccgatgata
     5101 tttatctttc aggaaaggca gactggataa cggaagaatt caggaatgac cttcacaggc
     5161 aggtggaatt attaagacac aatttgttgg gcatgacagc tcagaatctt gtaatggatt
     5221 catacaaagg tatatttgtt gcccttcatg atatcgaaaa agaatttacg gttttatatt
     5281 tctgggagcc gaactgcggc cactgcaatg aagcaacacc aaaactgaag gcttggtatg
     5341 agaaagcaaa aaatgacggg gttgagatat tttcggtctg taccacaagc aaccggcagg
     5401 aatgggctga ttatatagaa aaacacggac ttacctggat aaatggctgg gatcccaacc
     5461 ggcagacaca ctttgattac tattataatg ttcaggccac acccttgatt tatatactgg
     5521 acaaaaacaa aaagattata gcaaagaagc tgtcggttga tgatatacct tcattcattg
     5581 ataattacag aaaatatttc aggtaggtac attattgtac tgccggagta attttagcag
     5641 atcatacagg cttcccgaat tcctgacctc gtcaccatgg accagggacc atccctcctc
     5701 tccttccagc atcctgacaa acggttgtgt tttatcggct gagattctga aaccattcct
     5761 tctcaaggct ttaacggtcc agtttttctt ttttccctca ctgccttcca ggtatatatc
     5821 tgatgtgcat gccgtactga agctgaagct tccgtcttca ggattaaatc ccgtatgctc
     5881 ataatcaaag agacattcat aattatttgt cagccacaga tcttccgggg cgccctcaat
     5941 tatcccctta tctgttatca gccagatttt atcggcatta gtgactgcaa tattaaaatc
     6001 atgggtggaa aatactattg tcttgccaga agcggtcagt tccttcatca gtctgagcac
     6061 ttcaaacttt cccgagatgt caagaaaggc aaggggttca tccattatga gaatatccgt
     6121 attctgggca agtgccctgg ctatcatcgc cctctgccgt tcaccgtcag acagttcaga
     6181 aatgatcctg tctgaaaagc ctgccagtcc tgcctttttt atagaatcat taataatttc
     6241 atcagctgtg cggtcaattc tcccggtcca gtcagtatag ggaaagcgtc ccagtgcaac
     6301 caggtcatat accctcatat ggctcacctt tactatctct gttgaaatgt atccgacagt
     6361 cctggcaaat ttcaacctgg aatagtggct ggtatcttca tcccgtataa gcactgaacc
     6421 tcctatattt ttctgcaggc cggtaattgt tctgagaagc gtacttttcc cgataccatt
     6481 cctgccaaga accgcagtca gttccccttt ccctgccgaa gcaccgagtg gcggcaatac
     6541 gggtattttc ttcctgccat aacggtaacc aattatcaga gagtttacag tcaatatttc
     6601 ttcagtctgc tgtttcatca gaatagttcg gaacttatgt ttttcctcaa tataatccag
     6661 attaccaccg gaatacctat cagggaggta acggagttca ccggcagaat cttttctgat
     6721 cccggcagct gtgaaaggat atcgctgatg agcatgacgg tggctccggc aaaaacggtg
     6781 cccggcacaa gaaggcggtg atccgatgtt ctgagcatat tcctgacgat gtggggaact
     6841 gcaattccta tgaatgctat cggaccgcag aatgctgtca cgcttcctga cagaatactt
     6901 gtgcccgcaa aaataattat gcgtgcagca gttacattaa gaccggcact cgatgcataa
     6961 ttctccccta tcagcagtgc attgagcatt tttacggatg ccatgctcag gatcacaccg
     7021 gctgcaacag aaacagccaa taccttaagc tgttctgagg aaaggttgcc cagactgccc
     7081 attgtccaga ccacataggc cttcagcatg ctctcatcgc tgaaatactg aagaatactg
     7141 acaatggcgg agataccgct tgccagcatc ataccaatga taagtacggt cattatgctt
     7201 cgcaccctgg ctgatatgaa cagcatcagg atcatgactg cccctgcacc ggtccatgct
     7261 gcggaaacca gcagccagct cccgcctgca gtaactgatg ccgggcccat gcctgaagaa
     7321 aagcccagga taacaagcgc aacgccaagt ccggcacctg aacttacacc aagaacataa
     7381 gggccggcca tgggattcct gaaaacggtc tgcatctgta gtccgctcag tgacagggca
     7441 atgcctgcaa gaacagcagt aatggctttc ggaagcctga atttcagaat gagtgtctga
     7501 acagtttcat cgcccgtacc ggttaaggat cgtataacgt cagcaggcct gatggatact
     7561 gatcccagaa acaggtcaag accaaagagt ataagaagca gtacaataaa gcccgtgaag
     7621 atcaatttct gcaccggaag cactgaaggc gaaaaggtat ggctgatatg cccggtaata
     7681 ttttttccgt cagtcattca attttcctgt aataaaccat ctcataaccg gaaaaaattt
     7741 ccggatgaat tattgaggca atatccctga gaacaaggtg aggattaatc gctccgcttt
     7801 cccagtaatc atttccgcct gtaagattca gtcttttatt gttattatat atcctgttgt
     7861 taataaacgg aggcattgat cccagccgtt tatcaagagc agttatttca gctttggagg
     7921 aagcacttcc ggtattgagc cagatatcag ccttcattgc ggcagcatac accgtttcca
     7981 gactgtaggg cattgactgc gcagattctg tctctttcca taaatatctg ccgcctgcgt
     8041 catctataag tttgctgatg tacgaattac caggcgacac ataccaggta tcgcggaaag
     8101 gcattcccag cattattacc ggtttcggca cttttccggc cataagctcc ctgatttcca
     8161 gatatgagtc ccggatttcc ctgaacagcg aatcagcaag gtcttcacaa cagtacagtg
     8221 caccaaaaac ctttatccat tctgctttcc ccagggggtc agtttcaaga taatcagcat
     8281 tatacattac gttaaggccc agttcggcga atttggccgt atatccggca gattcgcttc
     8341 ctattccgta tatcataaca agatcaggcg aggatttaac aataagttcc gtattaagtc
     8401 ctgaatcata acctatatcg cttacaagtc cccggcttat cctgttactg atgtcgctgt
     8461 caaaaacaaa accggctcct gaaaccccga caatggaatt ctggcagccc agtgcccgga
     8521 tcattgcaat atgagtggtt gacatgcaga ttatcctcct taccgggaca cttattattt
     8581 ttgaagtacc ggctttaatt tccattctgc ccgtatccct taccaggtaa tattcatgaa
     8641 tcacattgcc cgcaccctgc cagggatctg taattctgag gatggtacag ctatcggtag
     8701 catgcaggtc aaagcgttcg gcatggctta tatttccccc ggtgtttttc tgtatttttt
     8761 cccttttccg gtctctactg caggaagaga aaatcatcag caggaaggaa acagacaaaa
     8821 acaggtataa caacttatat ctctgcactt ttacttattc ctgacaatac aatcacaata
     8881 caatacaata caatacaatt caactcaata taaatcacat caattcaatt cggtccgatt
     8941 ccgtttcata catcacagcg gccggccatc attcagtgtt tacaggagat caattggtga
     9001 atcagaagaa ttaaactctg agcttttctt cccgaaagca ttgaatagtg gtatccggca
     9061 ggtcttctga cttgtcccgg tccggcgaag ccttcccatt ccgtctgtcg gaacagtggc
     9121 agggtatcgc aggaccatta tcgcaggaac gatcgggcat cacagcagcg ggtactgtcg
     9181 cggtttctca ccgcattccc ttttcattgc agcatttgtc agatgctgca aaaccagaat
     9241 cagacaaata tatgactttt tatgtcatat atgacaggat gtggattttt gagtgaagag
     9301 tctgtctgag ggcatcctcc aggaggagta cggaaaccat tactctgata aacagctgtt
     9361 acagtgttcc gcaggaattt ctcatttcaa ggctgggcgg cagatttacc tgtcttttga
     9421 atttaccctt gcctgcattt tttatttccg accagatcat atttgctgct ttcaatgcaa
     9481 ggcctgaaac aggtttttta agacaggtta ccggagtgaa cataagatcg aatcccggtc
     9541 cgtcttccat cgatatcagt gcgatatcct gaggaacgcg cagcttttta tgccgcagca
     9601 ttgacatgac cggatacaca agttctgcat gcatcacgag aatgatatcg gccctgaagg
     9661 gaggccggag aaactgttct atctgattga aataatattc attatcagta agggaagctt
     9721 cgaactccac taccacaggt ttgtttacct gccggccttt ttccgtaagt gcggagatca
     9781 gctggttgat agcatcagta tcatgctttg ccgatttttt atcggttgca ataatgatat
     9841 tcttgtatcc gagtttattt acatggtctg ccacaagctg gatacccgca gccgtatctg
     9901 tattcacggt gctgagcctt cctgttttaa tgcttttttc aagaaggata aaagggcagg
     9961 atgctgttct cagagttctt atggtctgat catcggcagt ttcacccatc aggataagac
    10021 cactgaaaaa ttttctgaaa gcgccgatca ttctgtcata acgctgatca tcggggtctt
    10081 ttgttattat tgagaatccc agtccgatgc tgtggaatga tttctgcaga tatggcgtga
    10141 tgctctgaac aaaggaattt cctgatgaag gaactatcat tcctattatt cccggttttt
    10201 cgtcatgcgg cgttatctgc cccttttcct catgtgaact gaaatagccc atctgtctgg
    10261 ccattgccca tactttctcc tgtgtctctt ttcggattcc gtgctgatcg gccttattgt
    10321 tcagcaccag tgacaccagg gtaccagaaa cacccaatct ggcagcaaga tctttatttt
    10381 taaatttttt cattcctcta aactgtggtg aaaaataaat ttaggccttt tttggaagac
    10441 agttataaaa ataatgaatt tttcgctgtt tttttaaatt ttccttaaaa tattcatgtc
    10501 aaactcctca ggttaacagt catcttcagc gaaccagcac cttatctttt aggcggctta
    10561 tatcctctct gttttgcagc ctgttcaagc ctctgctgcc atttagattt cggcaggggt
    10621 ttcttcctgt tttcttccag tgtggccaat accttgtcag cgtcaataaa tctttttgac
    10681 agaacattct gcaaccatgt tatcatattg gcaagaaaat aataataagt cagtccggat
    10741 gagaaattat tgaaaatgaa caggaacata acgggcatca tgtacatcat taccttcatt
    10801 cccggctgat cctggcctga ggtggtcccc gacattctgg ttgtaacaat ggacgatatt
    10861 gtcatgagca gggtccacag gctcacatgg tcaccatacc agggaatttt aaagggcagg
    10921 ttgagaatgg aatcatatgt ggaaaggtct gttgcccaga ggaaggattc ctgtctcagt
    10981 tcaatggaaa ccggaaagaa tctgaacatt gcaaacaata tgggaaactg caggagcatg
    11041 ggcaggcagc cgcccatggg attcacacct gctctcttat agagatccat ggttgcctgc
    11101 tgcttcttca ttgcatcttc ctgtttggga tatttttttg ccagttcatc caccagtggc
    11161 ttaaggacct tcattttggc agttgactgg taggatttga aggtcagcgg gaaaagaatt
    11221 acttttatga gtattgtaag aataaggatg atgatgccat aactgctgat ataccgctca
    11281 agccagttaa atacaggtat aatgataaac ctgttgatcc aaccgacaat gttctttccg
    11341 acaaaaatta tcttgtccat atcatagcct tctttccgca gtgtcgagat atgattcgga
    11401 ccatagtaga atttcagggc gaggtttgtt tccgggccgt tttcaagggg gactcccagt
    11461 tcagaggtat agtaacggat atatttttct gacgtgagag tttttgcaga tgtaatggaa
    11521 gcgctcagga aataattatc cgtaatgagg accgaggaga aaaactgatc aacgtatgcc
    11581 acccagctta gcctggtggt gatgtcaacc ttttccactt ccttgtttga tctcagcctc
    11641 aatccgtcaa catcgtcctg atagtattta tacctgatgg tggaatacat atcctcattc
    11701 tgccttcctt tttcctgctg gggaatgtac attctccagt caagttcaat attattctgg
    11761 ttttcaggaa gaataccgtg catgttttta aaggtaatat cgtaatcgac actgttcttc
    11821 cccggagcga gggtatacct gtattcgata tatctttcat cgtctgcaac cagcctgagg
    11881 actacgctaa gcggttccga ttcaacaaaa tagtgtctgt tttcggaaag cggggtgaaa
    11941 tacagattat ttgtctgtac tgccttgttg tctgaggtga aaaagttgaa tccgaatacg
    12001 gttgaatcac ctgagaagag aatgagaggc ttgtcatcgt gtgttgtaaa atccttcacc
    12061 cgtgcggaat agacccggcc tcccttaagt gagattctga ggtctatctt gtcgttttcg
    12121 agtgttatat attcatcttc gccaagagct gatgcggcaa acacaccgta ttgcccggga
    12181 tcatccgcgg gtgttacgct cctgtctgtc ggtcttacgg cttcaggttc agcaactgca
    12241 ctgtcagcag gaatttcggt aactccggga attgtgccca gtttctggtt cagttcactg
    12301 atcctttcca gaacatccgg atgattgggt ttaaaccgga gtgcattgac atattcagtc
    12361 ctggcccgtt cgaaattacc cttgtcatat tcagcttccg ctgcagtcag tgctttttca
    12421 aattccttat taagcctgct gttattgtaa atactgaaac cgatgaagat caggaagatc
    12481 agtatcagtc ctgttattgt attcttgtcc attctagctg ttaatattat tttcttccct
    12541 cataaaaccc cagtccttct ttttatacag tggtctgcac aaatatgtga gcctgtcatt
    12601 ttgccggcac aaatatatcc ctaacatctg tcaaagcaat aaaaaatgtt tctgtttaag
    12661 ggcagcacta gcggttataa tcatcaattc ctgactggtc aatcattttg actttttcct
    12721 ccaggatctt gatcatcttt tttgcatttt caatctttcc ccttacctcg cgaatcatgg
    12781 tatcggcatt gggtgatttg gtaaaaaagc cgatattgtt ttcccagagc acaatatcat
    12841 tttccagctg ctttattttt gtgaaaaact tatcccgctc atttctgatt tttctagatg
    12901 ccttggggtt tgttttaagg ttttccagtc tggatctgaa cttgagaatg tttttatcct
    12961 catctcctat tttcagccgg tcaaattctt tgttcagtgc aatacgatac ttgtttgtaa
    13021 tctcctcctt cctgttgaag ggaacaaatc ctatttcggt ccatctgcgc tggatgtcct
    13081 taagtctgtc gaaggcttcc tgtacgtcgg ttcctgcctc aaactcttca agttccctga
    13141 tcagaacaag tttagccttt agattatctt catatgaagt atccagatca ctgaaaaatt
    13201 cagcttttct gtcaaagaag tgatcgcagg ctttccggaa tcttttccag agtttttcgg
    13261 attgtttcct gggaacgggg cctatttcct tccagctttt ctgaagtttt atcagtgcgt
    13321 ccgtggttcc tttccagtcg gtactgtcct gcaatgactc ggcctggaga cacagatcaa
    13381 ttttaagctg gagattgttc tgctgtattt ccctgttatc ggcatagaaa ttcctttttt
    13441 tgtcgaaaaa gctgtcacag gcatcccgga atctctggta tactttattg ttttgctttt
    13501 ttggcgcaaa gccgattgtt ctccacattt tctgcagcgc catgattttt tctgcctttt
    13561 cgtcaaaatc cttgaaattt ttgatttcca gaagatttat ttcctccact tcttcacaca
    13621 gggctatctt tgcctccagg tttctgcgct gaacatcttt ctggttctcg aaatattcat
    13681 gatgcctctt gtttatctgt gatgttgctt ccctgaagcg ttcccatatt tcatttttcg
    13741 attcaacggg tacaggccct atttcacgcc agtgattatg gtattcctgc aggatccgga
    13801 aggcatttac cggatttggc tcaaggagaa gttcttcggc cttttcacat aacagcacct
    13861 tggcttcgag attctttttc agatcaaggt cacgtaactc tttattgatt ttaatataat
    13921 cataaaaaat ttccacgtga tgatggtaat tctcccagag atgcttcagt gcgttctggg
    13981 gtaccacgcc taccgaatgc cactcattct ggagtgacct gaattcattg aaggttttat
    14041 ttattgattc ctcacggttg acaagttcct ttatcttgtc gattatctca tatttcttct
    14101 tgaggttctc atatttttca gcttcctgta ttctgttgaa gtcagttttt ttctgcctgt
    14161 acttatcaag cagatgttta acctgtgctt caagcggatc gacccatgac cggtaatcct
    14221 cgagagtacc gccgccttcc aggaatttgc tttttctttc ttcagcttcc tgcttcagct
    14281 ttttatagaa atagaccttt atcctgtcca catcgctgcg gatctctgac gggggcctgt
    14341 tctcgatcag aagagccagt gtctctacca gatcattctt cgagaaaccg gaataatcaa
    14401 ccggaggcag ttcaatatca tcaagatttt caatcccgct ctcgtattcc tcgattccgg
    14461 aatccctttc agactcctgt accgttttcc tccttctttt tacaggaacc ccgccttccc
    14521 tctcagtctg gtccccttcc ggaagttccg cttccgaatg ttcatctgct gacttttctt
    14581 ctcccggctg ttctttctgc tgatctgtaa tactggcttc cggtaattct tcagtatttc
    14641 tatttctctc agttccggca gcgttttcag gtgccccgga ttgttcatca cgaacagagt
    14701 catccggatc tttatctatt atcatagcaa gtcctttcta att
//
LOCUS       DAOSPX010000041        14376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4287_length_14376_cov_3.689407, whole genome shotgun sequence.
ACCESSION   DAOSPX010000041 DAOSPX010000000
VERSION     DAOSPX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4287_length_14376_cov_3.689407"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLZ75_03565"
     CDS             complement(<1..383)
                     /locus_tag="PLZ75_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOS71509.1"
                     /translation="MAACVEQQSDKFEAARNRMVKIQIQNRGVTNSAVLDAMRKVPRH
                     LFVPDEYQSEAYNDYPLPIGYGQTISQPFIVAYMTEAVRPSGNMKALEIGTGSGYQAA
                     VLAELVKEVYTIEIVPELAAESAGR"
     gene            complement(521..1159)
                     /locus_tag="PLZ75_03570"
     CDS             complement(521..1159)
                     /locus_tag="PLZ75_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOS71510.1"
                     /translation="MDKFDILRTLKFLPGIILGLTVHEFSHAFVAHRCGDPTSKDQGR
                     VSLNPLKHIDPMGFIMLLVVGFGWAKPVEFNEQNLRNPRTDTLKIAVAGPLSNALFAM
                     LLSVLFVIVFRLFNIAYYSITTEILIYAIYINWGLFIFNLLPVPPLDGSHLLLSFFRK
                     YPVFYNGLYKYGSYFFLGLIVLSLVSGLNLFPIWSLVQFLGNGFLSLFGFTL"
     gene            complement(1146..1946)
                     /locus_tag="PLZ75_03575"
     CDS             complement(1146..1946)
                     /locus_tag="PLZ75_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71511.1"
                     /translation="MNRKLYLLLLLYFVISTHCRRDEPFPVTDLHIHLKGNFGMEDAI
                     AKSQAENISYGIAFNCGLKFPVHSDSQIDSVITMMKDYPQFYAAMQAEGREWVNIFSR
                     ESINKFDYVFTDAMTFTDAKGRRNRLWLKEETWIDDEEEFMDYLVSTTVNILLEEPID
                     IYVNPTFLPAQMAGRYNEFWTAERTDRVIQAARAANIAIEINNRYRIPSADFIKKAKK
                     AGLKFTVGTNNADENFSGAEYALEMIKKCRLTRDDFYLPVNKRLNGQI"
     gene            2081..3265
                     /locus_tag="PLZ75_03580"
     CDS             2081..3265
                     /locus_tag="PLZ75_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOS71512.1"
                     /translation="MEKLFSVFSAIVLFFSAGIYAQDREVPEVYLLTCGPGTETYSRW
                     GHSALRIVDSMEGTDRVYNWGVFDFNTPAFAWKFARGRLEYMLGVSTYERFIQEYFLE
                     GRWVQSQKINLEPRETAELMHLIGINLRPENVKYRYDFFYDDCSTRIRDLLEEAIGDS
                     MVYPPEIFDEAPSFRFLTGKYLMTAPWLKLGIDLLMGSPGNKKASVRDRMFLPLDMQK
                     GLSDIVINRNGKMIPLLQNPVTVLEKSLKTEKTSFLTSPLFVFSILLVIVIILSAVFR
                     DLKSNRIIDILLFSVFSVLALMMIFFNFFTDHQQMRLNANAVWLNPFIILCLLSVILK
                     RNWHTWFRMVFVLNVLWFFVLLIFPHSSNTAFMPLVLVLIVRSSARAGYPWNPLTLPE
                     EE"
     gene            3353..4348
                     /locus_tag="PLZ75_03585"
     CDS             3353..4348
                     /locus_tag="PLZ75_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS71513.1"
                     /translation="MEQTADIKILNEKIEKESAFVDIVRMEMNKVIVGQKHLIDSLLI
                     GLLGNGHILLEGVPGLAKTLAIKSLAAVIDAKFSRIQFTPDLLPADLIGTMIYSQKKE
                     EFTVKKGPVFANFILADEINRSPAKVQSALLEAMQERQVTIGESTFSLEEPFLVLATQ
                     NPIEQEGTYPLPEAQMDRFMLKVLITYPSMEEEKLIIRENLAKEFPKTNTVIRPSDII
                     RARDVVREVYMDEKIEKYILDIVFASREPEKYGLTQFRNMISYGASPRASIYLSVAAK
                     AYAFIRRRGYVIPEDVRALCPDVMRHRIGLTYEAEAENMNQDKIIAEILNVVEVP"
     gene            4433..5296
                     /locus_tag="PLZ75_03590"
     CDS             4433..5296
                     /locus_tag="PLZ75_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOS71514.1"
                     /translation="MEATDLLRKVRRIEIKARGLTRHIFAGEYHSAFKGRGIAFSEVR
                     EYHYGDDIRNIDWNVTARFNHPYVKIFEEERELTVMLLIDVSGSGRFGTSISFKQDLM
                     TEVAAVLSFSAIFNNDKIGVIFFSDRIEKFIPPQKGRKHILVIIRELLSFRPESRKTD
                     LDVPLRFLTNAIKKRCTAFILSDFFAPGFEEALRIASSKHDIAAIKVYDPAETGIPDI
                     GMLKISDAETGAERWIDTSSKESRNAYARWWDKHVSAIDGIFKKCGVDSAFISTAEDY
                     VKPLIKLFEKR"
     gene            5293..6276
                     /locus_tag="PLZ75_03595"
     CDS             5293..6276
                     /locus_tag="PLZ75_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71515.1"
                     /translation="MKFQIHIYTGLFLFSCLLPLDAQEVNVSARFDTSRIYIGDQINY
                     TVIVDQPAEISLDIVSAKDTLVKGIEVLSGPLTDTLALDNGKLRIVQNYLITSFDSGF
                     YEVPPRYAELILESGIRRYYSDYAALEVVRVNITPPDTASGIFDIIGPYGAPLTFGEI
                     VPWILLAALSAVLIWVVLRLMKRFRKARPGYEPVIIREAAHVIAFRELEKLRDQKLWQ
                     KGEVKHYFSRLTEIVRQYLEDRYGVFSLELTTSETLEELLKTGFKKDETYIILRDILN
                     AGDLVKFARYKPDPSENEFHFENSWKFVDMTRQREIPEEQVPGNNDKGDKQ"
     gene            6282..7265
                     /locus_tag="PLZ75_03600"
     CDS             6282..7265
                     /locus_tag="PLZ75_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOS71516.1"
                     /translation="MTFANPVFLWLLVLVPVIVAYYVIRQHKTVSSLTMPHIDAFGNA
                     GKSFRNWLRHLLFGFRVLVIGLLIVAAARPQKSDRFQDVSTEGIDIIIALDISGSMLA
                     RDFRPDRLEASKNVATEFISGRPYDRIGLVVFSGVSFTQCPLTTDHAVLINLMRELKS
                     GMIEDGTAIGNGLATAISRIKDSGSKGKVIILLTDGVNNRGEIAPATAADIAKVYGIR
                     VYTIGVGTLGTAPFPVQTPYGTQIMNMAVEIDEGILKEISAKTGGKYFRATDNNKLVE
                     IYAEIDKLEKSRIEVRQYIRKEEKFLLPALIAFILLAVDMLARYTIYKKIT"
     gene            7277..8317
                     /locus_tag="PLZ75_03605"
     CDS             7277..8317
                     /locus_tag="PLZ75_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOS71517.1"
                     /translation="MQLFRFANSAFLYLLLLLPVMILLWILNERRKRKALRKFGNTDL
                     VKKLMPEYSAVRPGLKFFFSLLAMGLIILVLARPQFGSKLEEVKKQGVEVIIALDVSN
                     SMLAEDIQPDRLTRAKQALNRLIDKLENDRIGLIVFAGDAYTQIPITTDYISAKMFLS
                     SISPEMVPKQGTAIGSAIALGMRSFTPGEDKSRAMIIITDGENHEDDPVAAAEEAARA
                     GIVIHTVGIGSVEGVPVPLSVGGRKDYLKDHEGNTVITKLDEETLKKIAVSTSGTYVR
                     AGVTSIGLDEVFNEIRKMKKQDLESKMYTEYNDQFRIFAIAALIFLIAEFLIMDRKNR
                     KLADIKLFRYKI"
     gene            8331..9086
                     /locus_tag="PLZ75_03610"
     CDS             8331..9086
                     /locus_tag="PLZ75_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS71518.1"
                     /translation="MNHKNKYHNKLLRQLSVLILAVLSVPPSAYSQGDKKYIREGNRE
                     YEKANYADSEISYRKALDKNNSSGNAVFNIGNALYKQEKYEDAGRQYSTNFEMNDDRR
                     KKSASLYNLGNSLLKGGRIPESIEAYKRSLKLDPRNMEAKYNLAFAQDLLKQQEEQQQ
                     QQQNQNQEQENQDDRQQQEQQRQDNEQDQQQQDNKQDQQQEQQQEQDRESQISREDAE
                     RILNSLANDEKDIQEQLKLDKAKQQKVKTLRNW"
     gene            9105..10934
                     /locus_tag="PLZ75_03615"
     CDS             9105..10934
                     /locus_tag="PLZ75_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOS71519.1"
                     /translation="MKKLLIYNLLFLITANLSAQDIVVEAEYPRTVRAGEQFAIQWRI
                     NARGGDFTAPSFAGFIRLMGPQTSYSSSTQIINGRVTHETSESYLFYLQAVDEGIFVL
                     PPASVTIKNKTYHSDSVRIVVTGGSAPPAAGQPGTAGQQGAAGQQQRTSPSSPPEGEI
                     SLDLSVNRREVYMGEPVSVTVKLYTRVDISGYNEIKYPSFTGFLKTDLETPPLSSLRQ
                     ENINGTIYGTGVLQQFLLYPQITGEITIDPVQITVLVRQRSGRSDPFFGDFFSTYTTV
                     PRAVASTPVKITVRPLPGIKPEDFSGIAGKISIKAGINRDTINVNDALNYKITISGTG
                     NLRLAGIPALKLPPDIEVYDPKVTEDLRNTVNGTSGQKIFEYLLIPRHHGDYVIPAVS
                     YSYFNVSTGKYEHLRTPEFRFHARQGSAQGSEIAVFGGVAKEDVRYLGKDIRFIRTNP
                     GKLSKPGRQILSQNYFYLAYVITLFLFLVILFLRREHIRRNADIAAVKNRRAGKVAAK
                     RLKAASDCMKKGETDRFYEEILKAIWGYLSDKLSIPVSDLTRNSAMDALQKRGIDDEK
                     IKNLADILDKCEYARYAPASSGTAVPEIYNDASGFIRSVENEI"
     gene            10937..11707
                     /locus_tag="PLZ75_03620"
     CDS             10937..11707
                     /locus_tag="PLZ75_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71520.1"
                     /translation="MNFKSSLIPLILLLLFVIPDAGAQDPAAERFNRGTEYFSEGNYE
                     KALEEWTGLYTAGYRSATLDYNIGNAYFKLGNIPGSILFYERARLRKPADENIRYNLG
                     IARAMTTDRLDEIPELFFVRWYDFLSLILSSDNWAVISLVSFIICLAFLSLYFYSSKY
                     NMKVAGFWLAVLALLISLASLSFSLRNKRLIYESNKAIIFSPIVNAKSSPDDSGTDLF
                     VIHEGLRVSVEDEVGEWKEIRLPDGNKGWVPANSLEII"
     gene            11771..12187
                     /locus_tag="PLZ75_03625"
     CDS             11771..12187
                     /locus_tag="PLZ75_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71521.1"
                     /translation="MRKNICLLFVLLALSACKKEKWEPEGPTDVRIRNLQTDELMADV
                     VINTAGVRDTVGNVKKLGMVKPGRTSEYRRVSIAFPKAEITAEINGETFSTGPVNSTY
                     MHYIGLMRITYEVQIKDPEKRLLEIKNIIPDEALKP"
     gene            complement(12337..13002)
                     /gene="ung"
                     /locus_tag="PLZ75_03630"
     CDS             complement(12337..13002)
                     /gene="ung"
                     /locus_tag="PLZ75_03630"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOS71522.1"
                     /translation="MDVKIESSWKEKLKDEFSKDYFISLTGFVREEYGKTQVFPPGGL
                     IFKAFDLCPFDRVKVVIIGQDPYHGPGQAHGLCFSVREGIDFPPSLVNIFKEIESDLG
                     IKPYSSGNLSRWAAQGVFLLNATLTVRARQAASHQGKGWEEFTDAAISVLNNDRDNLV
                     FFLWGAYAQKKGTSIDRNRHLVLESVHPSPLSASRGFFGNRHFSRCNKYLTDHGIAPV
                     DWR"
     gene            complement(13017..13493)
                     /locus_tag="PLZ75_03635"
     CDS             complement(13017..13493)
                     /locus_tag="PLZ75_03635"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS71523.1"
                     /translation="MRISEGIGDEDIPGLEKILASTGYFYDFEIRTALEIAVQTVSQG
                     MDASGYFWIRITEGEQLIGFACYGKNDFSVHSWELYWIAVHQDFRNHSYGRALLQAAE
                     ERIRQKGGKILWIETSGRPLYKPTEEFYKKNGYRLEVSLQDFYGPGDPKQIYARRL"
     gene            complement(13501..>14376)
                     /locus_tag="PLZ75_03640"
     CDS             complement(13501..>14376)
                     /locus_tag="PLZ75_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOS71524.1"
                     /translation="KNLSEIGVETFRTGITVDIMKEIAGLAAGKVDFVFNLVESIIGK
                     GELCYFIPALLNAYSIPYTGNPVEAIFVTTSKAFTARSLKQAGINNPGGFAPSQSGQL
                     IPGNTYIIKPVWEDGSLGITSDSVFTCTPEIIKKLKKYDDTHWLIQDYIEGREFNISI
                     ISGETGPEVMPAAEMIFHDSWDGKPKIVDFKAKWEEDSFEFQNTLRHFPGDKLDKALE
                     KQICDIALKCWKIFGLRGYARIDLRVDKNNIPYVIEVNANPCLSPDSGLVAATRVAGL
                     KFSEVIRRIITDLNN"
BASE COUNT         4153 a         3160 c         3288 g         3775 t
ORIGIN      
        1 aacctgccgg cagattccgc ggcaagttca ggaacaattt caattgtata gacttctttc
       61 accagttctg ccagaaccgc tgcctgatat ccggatcctg ttccgatttc cagagctttc
      121 atgttccccg acggccgtac tgcctctgtc atatatgcca caataaatgg ctgtgaaatg
      181 gtctgtccgt aacctatagg caggggataa tcattgtatg cttcactctg atattcatcc
      241 ggaacgaaaa gatggcgggg aactttacgc attgcatcaa gtaccgcaga attagtcacg
      301 cccctgttct gaatctggat ttttaccatc cggtttctgg cagcctcgaa tttatcagat
      361 tgctgttcaa cacaggctgc cataatcaaa atctgaatta atataaatat tttcacaatg
      421 acctcctttc ctgcaatata tttaaatttc atcaaattac aaagggatga ttcgccccgt
      481 tccggcagct gaagcaggaa ttttcattga attgttcctt ttacaaggta aatccaaaca
      541 gcgacaggaa accatttccc agaaactgaa caagtgacca tatgggaaaa aggttcagcc
      601 cggaaaccag cgacaggaca atgagaccca ggaagaaata gcttccgtat ttatatagtc
      661 cgttgtaaaa gacaggatat ttcctgaaaa aggaaaggag aagatgtgat ccgtcaagcg
      721 ggggaaccgg gagaagatta aagataaaga ggccccagtt gatatatatt gcgtagatga
      781 gaatttctgt agttattgaa taataggcaa tattgaaaag cctgaaaaca atcacgaaca
      841 gaacagaaag cagcatagca aaaagagcat tggaaagcgg tccggcaaca gcaatcttaa
      901 gcgtgtcggt ccggggattg cgcagattct gctcattgaa ttccaccggt ttagcccaac
      961 caaaaccgac aacaagcaac atgataaatc ccatgggatc aatgtgcttc aggggattta
     1021 gagaaaccct gccctgatct ttcgaagtag ggtcaccaca cctgtgggca acaaacgcat
     1081 gactgaactc atggactgta agaccaagaa taatcccggg cagaaattta agagtcctca
     1141 gaatatcaaa tttgtccatt tagtcttttg ttaacaggca gataaaaatc atccctggtc
     1201 agtctgcatt ttttgatcat ttccagagca tattccgcac ccgaaaaatt ctcatcggca
     1261 ttatttgttc caacagtgaa tttcagccct gccttttttg cctttttaat aaaatccgca
     1321 gaaggaatcc tgtagcggtt atttatctca atggctatat tggcggctct ggcagcctgt
     1381 atcaccctgt ccgtcctttc cgctgtccag aattcattat aacgccccgc catctgtgca
     1441 ggcaggaaag tgggatttac ataaatatca atcggttcct caagaagaat atttactgtt
     1501 gtactgacaa gataatccat aaattcctct tcatcatcta tccatgtctc ctcttttaac
     1561 cacagcctgt tccttctccc ttttgcatcg gtgaaggtca tggcatcagt gaaaacataa
     1621 tcgaatttat ttatggattc cctggagaaa atatttaccc actcccttcc ttcagcctgc
     1681 attgcagcat aaaactgagg ataatccttc atcatggtaa tcacagaatc aatctgactg
     1741 tcagaatgaa caggaaactt cagtccgcag ttaaatgcaa ttccgtaact gatattttcc
     1801 gcctgtgatt tggcaatggc atcttccatg ccgaaatttc ctttcagatg tatatgaaga
     1861 tccgttacag ggaatggttc atccctcctg caatgtgttg atattacaaa atataacaaa
     1921 aggagaaggt ataatttccg gttcatgaga agaggtatta attattttcc ggcaatttaa
     1981 taaaatttaa aaaaatgctg tccggtttgc tgctatatca ggacttattt gcactttcgt
     2041 agctgtaatg aagaattcat aaataattaa tctgtaatcc atggaaaaac tgttttcggt
     2101 attctcagca attgtattat ttttttctgc tggaatttat gcgcaggaca gggaggttcc
     2161 tgaggtatat ctgctgacct gcggtcccgg aactgaaact tattcacgct ggggtcacag
     2221 tgcactcagg atagttgaca gcatggaagg tactgacagg gtttacaact ggggggtttt
     2281 tgatttcaat actccggctt tcgcgtggaa atttgccagg ggcagacttg aatacatgct
     2341 tggcgtttca acttatgagc gtttcatcca ggaatatttc ctggaaggaa gatgggtaca
     2401 gtcacagaaa ataaatcttg aacctcggga aacggcggaa ctgatgcatc tgataggtat
     2461 aaatctcagg cccgaaaacg taaaataccg ctatgacttt ttttatgatg attgttccac
     2521 acgtatcagg gatctgctgg aagaggcgat aggggatagc atggtgtatc ctcctgaaat
     2581 ttttgacgag gcgccttcat tccgctttct gaccggaaaa taccttatga cagcaccatg
     2641 gctgaaactg ggaatagacc ttcttatggg atctcccgga aacaagaagg catcagtacg
     2701 tgaccgtatg tttctgcctc tggatatgca gaaaggcctt tcggatattg ttattaacag
     2761 aaacggtaaa atgattccgc tcctgcaaaa tccggtaacc gttctggaaa aaagcctgaa
     2821 gaccgaaaaa acaagctttt taacatcacc cttatttgtg ttctcaatcc tgctggtaat
     2881 tgttatcatt ctttccgcgg ttttcaggga ccttaaaagc aaccggatca ttgatatcct
     2941 gttgttctct gttttctcgg ttcttgcatt aatgatgatc ttctttaact ttttcaccga
     3001 tcatcagcaa atgcgcctga atgccaatgc agtctggctt aatcctttta ttatcctctg
     3061 ccttctttca gtgatcctga aaagaaactg gcatacatgg ttccggatgg tattcgttct
     3121 gaacgtgctg tggttttttg tattgctgat ttttccgcat tcaagcaaca cggcctttat
     3181 gcccctggta ctggtgctta ttgtcagaag ttcggcaaga gcaggatatc cctggaatcc
     3241 cctcacgctt cccgaggagg aatgagcctg ttttaacaga tatttaacac tccttaacac
     3301 ctccttatta attacagggc tttttttgta ttgcctttta aagattcata ttatggagca
     3361 gacagccgac attaaaatac taaatgaaaa gatcgaaaag gaaagtgctt ttgttgacat
     3421 tgtcagaatg gaaatgaaca aagttattgt tggacagaag catctgatcg acagcctttt
     3481 gatcggactt ctggggaacg ggcacattct tctggaggga gttcccgggc tggcaaagac
     3541 tcttgccata aaatccctgg ctgccgttat tgatgcaaaa ttcagccgta ttcagtttac
     3601 acccgatctt cttccggctg accttatagg tactatgatc tacagccaga agaaggaaga
     3661 gttcactgta aaaaaaggtc cggtctttgc caattttatt ctggcagatg aaatcaaccg
     3721 ttcgcctgca aaagtgcaga gtgcacttct cgaggccatg caggaacggc aggttactat
     3781 tggggaatcc acattctcgc tagaggagcc tttcctcgtt cttgccacac agaacccgat
     3841 agaacaggaa ggtacctatc cgcttccgga agcacagatg gacaggttta tgcttaaggt
     3901 actgatcact tacccctcaa tggaggagga aaaacttatt atcagggaaa atcttgcaaa
     3961 agagtttccg aaaacaaata cggtaatcag gccttcagat attatccgcg caagggacgt
     4021 tgtcagggaa gtgtacatgg acgaaaaaat agaaaaatat attctggata ttgtttttgc
     4081 aagcagggaa cctgagaaat acggtcttac acaattcagg aacatgattt catacggagc
     4141 atcacccagg gcttcaattt atctgtctgt tgcggccaag gcatacgcct tcataagaag
     4201 aaggggatat gtgatcccgg aagatgtcag ggctttgtgt cccgatgtta tgcgccacag
     4261 gatcggcctg acctatgagg ctgaagcaga aaatatgaat caggacaaga taattgcaga
     4321 aatactgaat gtagttgagg ttccgtaaga cacgggggga ggatcaaacc ctcaggatat
     4381 gcggcctgcg cagtgaatca ggaataatac tgaaggaatt actttgctga aagtggaagc
     4441 aacggattta ttaagaaaag taaggaggat tgaaataaag gcacgcggac tcacgagaca
     4501 tatttttgcc ggtgagtatc acagtgcatt caagggaagg ggtattgcct tcagtgaagt
     4561 aagggaatac cattacggcg atgatatcag gaatatcgac tggaatgtca ccgcacgctt
     4621 caatcatcct tatgtcaaaa tatttgaaga ggaacgggag cttaccgtaa tgcttctgat
     4681 cgatgtaagc ggatcaggaa gattcggaac cagcataagc ttcaaacagg atcttatgac
     4741 cgaagtggca gcggtactgt ccttctctgc aattttcaac aatgacaaga tcggtgtgat
     4801 cttcttttct gacagaatcg agaagtttat tccgccccag aaagggagaa agcatatatt
     4861 ggttataata agggaattgt tgagtttcag gcctgaaagc cggaaaacag atctggatgt
     4921 tcccctgaga tttcttacca atgcgataaa gaaaagatgc acagctttca ttctttctga
     4981 tttttttgcc cccggttttg aggaagctct gagaattgct tcaagcaagc atgatatagc
     5041 agcaatcaag gtatatgatc ctgccgaaac cggaattccc gatatcggaa tgttaaaaat
     5101 ttccgatgct gaaacaggtg cggaaagatg gatagatact tcctcaaaag aatccaggaa
     5161 tgcctatgcc cggtggtggg ataagcatgt atcagccatt gatggcatat ttaaaaaatg
     5221 cggagtcgat tcggcattta taagtacagc tgaggattat gttaaaccgc tgataaaact
     5281 gtttgagaaa cggtgaaatt tcagatacat atttataccg gcctcttcct gttcagctgc
     5341 cttttacctc tggatgccca ggaagtaaat gtgagtgccc gttttgatac atcacggatt
     5401 tatatcggcg accagatcaa ttatactgtt atagttgacc agccggccga aataagtctt
     5461 gacattgtat ctgcaaagga taccctggtg aaaggcattg aagtcctctc gggtccgtta
     5521 actgatacac tggcactcga taacggcaaa ctgagaattg ttcagaacta ccttataaca
     5581 tcatttgatt caggattcta tgaggtacca ccccgttatg cggaattaat tcttgaatcg
     5641 ggaatcagga gatattattc tgattatgct gccctcgaag tagtaagggt gaatattaca
     5701 cctcctgaca cggcatccgg aattttcgat ataatagggc cctacggtgc acctctcaca
     5761 ttcggggaaa tagtgccgtg gattcttctg gcagcccttt cggcagtgct gatatgggta
     5821 gtgttgcggc ttatgaaacg gttcaggaag gcccggccgg gatatgaacc tgttatcatc
     5881 agggaagcgg cacatgttat tgctttccgt gaacttgaaa aactcaggga ccagaaatta
     5941 tggcagaaag gtgaagtcaa gcactatttt tcaaggctga ccgaaatagt cagacaatac
     6001 ctggaggacc ggtacggagt cttttccctg gaactgacta cttcggaaac tctggaagag
     6061 ttgctgaaaa ccggttttaa aaaagacgaa acctatatca ttttacgaga tattctgaac
     6121 gcgggagatc tggtgaaatt tgccagatac aaacccgatc cttcggaaaa tgagtttcat
     6181 tttgaaaact cgtggaaatt tgtcgacatg acaaggcaga gggagatacc agaggaacaa
     6241 gttcccggaa ataacgataa gggagataaa caatgaaagg aattacattt gcaaatcctg
     6301 tatttttatg gttgctggtg cttgttccgg tgattgttgc ctattatgta atcaggcagc
     6361 ataaaactgt ttcctcgtta acaatgcctc atattgatgc tttcggaaat gccggaaaga
     6421 gtttcaggaa ctggctgagg catcttctgt tcggtttcag ggtgcttgtc attggtcttc
     6481 tgattgtggc ggctgcccgt ccccagaaat cagacaggtt tcaggatgtg agcacggaag
     6541 gaatagacat tatcattgcc ctggatattt cgggaagcat gcttgcgcgc gacttcaggc
     6601 ccgacagact ggaagcctca aagaacgtgg caactgaatt tatttccgga agaccatacg
     6661 accgcatagg actggttgtt ttcagcggtg tgagctttac ccagtgtccg ctcacaaccg
     6721 accatgctgt cctgataaac ctgatgcggg agcttaaaag cggcatgata gaagacggga
     6781 ccgcaatagg aaacggactg gcaacggcta taagcagaat aaaggattcc ggatcgaaag
     6841 gaaaggtgat cattcttctt acagacgggg taaacaacag gggtgagatt gccccggcca
     6901 ctgctgcaga tattgcaaaa gtctatggca tccgggtcta taccatcgga gtgggaaccc
     6961 tgggaacggc tcccttcccg gtccagacac cctacggaac ccagatcatg aatatggccg
     7021 ttgaaattga tgaaggcata ctgaaggaga tctctgccaa aacaggagga aaatatttcc
     7081 gggcaacgga taacaataaa cttgttgaga tctacgctga aatagacaag ctggagaaat
     7141 cgagaatcga agtgcgtcaa tatatacgga aggaggaaaa attcctgctt cccgcactga
     7201 ttgcctttat tcttctggct gttgatatgc ttgcaagata tactatttat aaaaaaatta
     7261 cctaaacgag agcaaaatgc agttattccg gtttgcaaat tccgctttcc tgtacctgtt
     7321 gcttctgctt ccggtaatga tcctgctttg gatactgaat gaaaggagaa aaagaaaggc
     7381 tctccgcaaa ttcggaaaca ctgatcttgt aaaaaagctc atgccggaat attcggcagt
     7441 gagaccggga cttaaattct ttttcagtct gctggcaatg ggacttataa tcctggttct
     7501 tgccagaccc cagttcggat caaagctcga ggaggtgaaa aagcagggag ttgaggttat
     7561 tatagccctt gatgtcagta attcaatgct tgcggaggat attcagcccg acaggctcac
     7621 cagggccaag caggccctta acagacttat tgacaagctc gaaaatgaca ggatcggatt
     7681 aattgttttt gcaggcgatg cctatacgca aattccgatt accactgatt acatttcggc
     7741 aaaaatgttc ctctcctcca tcagtcctga aatggttccg aagcagggaa ccgccatagg
     7801 ctcggcaata gccctgggaa tgcgctcctt cacccccggg gaggacaaga gcagggccat
     7861 gataatcatc actgacggcg agaatcatga ggatgacccg gtagctgccg cggaagaagc
     7921 cgccagggcc gggattgtca ttcacactgt gggaatagga tcggtggagg gtgtgcctgt
     7981 tccgctttct gtaggaggca ggaaagatta tctgaaggat catgagggca ataccgttat
     8041 tacaaaactt gacgaggaaa cactgaagaa aattgcagta agcacttcag gaacctatgt
     8101 ccgggccggt gtcaccagca tagggctcga tgaggttttt aatgaaataa gaaaaatgaa
     8161 aaaacaggat ctggaaagta aaatgtatac agaatacaac gatcagttcc gtatttttgc
     8221 aattgcagca ttaatattcc tgattgccga attccttatt atggacagga agaacaggaa
     8281 acttgctgat attaaattat tcagatacaa aatatgatat tcccgggcat atgaatcata
     8341 agaataaata ccacaacaaa cttctccggc agctgtctgt cctgattctc gccgtgttgt
     8401 ccgttccgcc gtcagcatat tcccagggag acaaaaaata tatcagggag ggaaaccggg
     8461 aatacgaaaa ggccaattat gctgattccg aaatatcata ccggaaggcg ctggataaga
     8521 acaattcctc cggaaatgct gtttttaaca ttggcaatgc cctttacaaa caggaaaagt
     8581 atgaagatgc gggcaggcag tacagcacta actttgaaat gaatgacgac aggcggaaaa
     8641 aatcggccag cctgtataac ctcggcaatt cccttctcaa gggcggcagg attcccgaaa
     8701 gcatcgaggc gtataagaga tcccttaaac tggatccgcg caacatggaa gcaaaatata
     8761 accttgcctt tgcccaggat cttctgaaac agcaggaaga acagcaacag caacagcaga
     8821 atcagaacca ggaacaggaa aaccaggatg acaggcagca gcaggaacag caacggcagg
     8881 ataatgaaca ggatcagcag cagcaggata ataagcagga tcagcagcag gaacagcagc
     8941 aggaacagga ccgggaatcg cagatatcca gggaagatgc ggaaaggata ctgaactccc
     9001 ttgccaatga tgaaaaggat atccaggaac agctgaaact ggataaggca aaacagcaaa
     9061 aagttaaaac tctcaggaac tggtagcacg acaggcacga aagaatgaaa aagttgctga
     9121 tatataatct tctgtttctc ataactgcaa atttaagcgc tcaggatatc gttgttgaag
     9181 cagaataccc gcggactgtc cgggcaggag aacagtttgc catacagtgg cgcataaatg
     9241 caagaggagg ggatttcacc gcaccctcct ttgccggttt tatcagactg atggggcccc
     9301 agacatctta cagttccagc acacagatca taaacggccg ggtgacccat gaaacatcgg
     9361 aatcatacct gttttacctt caggcagttg atgaaggaat atttgttctg cctccggcct
     9421 ctgtcacaat caaaaataaa acctaccatt ccgattcagt ccgaatagta gtaacgggag
     9481 gcagtgctcc cccggctgcg ggacagccgg gaactgccgg tcagcagggt gctgccggtc
     9541 agcagcaaag aacatccccg tcttctcctc ctgaagggga gatatcgctt gatctttccg
     9601 taaacaggag agaagtctat atgggagaac ccgtatccgt gacggtaaaa ttatatacca
     9661 gggtcgatat ttccggctat aatgagatta aatatccttc gtttaccgga ttccttaaaa
     9721 ccgatcttga gacacccccg ctcagctccc tccgtcagga gaacataaac ggaaccattt
     9781 acggaacggg tgtgctgcag cagtttcttc tctatccgca gattaccggg gagattacca
     9841 ttgatccggt tcagataacc gtcctcgtcc ggcaaagatc aggaagatcg gatcctttct
     9901 tcggcgattt cttttcaaca tatacaaccg taccccgtgc tgttgccagc acaccggtaa
     9961 aaataacggt cagacccctc cccggcatca aaccggagga tttctcgggc attgccggta
    10021 aaatcagcat caaggcaggc ataaacaggg ataccattaa tgtaaatgat gccctgaact
    10081 ataaaataac aatatccgga acaggaaatc tcaggctggc cggtataccg gctctgaagc
    10141 ttcctcccga catagaggtc tatgatccga aagtaacgga agacctcagg aataccgtta
    10201 acggcacaag cggacagaaa atatttgaat atctgcttat tcccaggcat catggagatt
    10261 atgtcattcc tgcagtatcc tattcatatt ttaatgtttc aacaggaaaa tatgaacatc
    10321 tcagaactcc ggagttcagg ttccatgcca gacaaggcag tgcacagggc tctgagatag
    10381 ctgttttcgg aggagtggca aaggaagatg tgcggtatct gggcaaggac atacgcttca
    10441 tccggacaaa cccgggaaag ctttccaaac ccggcagaca gatactgtct cagaattatt
    10501 tctacctggc ctatgtgatc actttgttct tgttccttgt cattttgttc ttaaggcggg
    10561 aacatatccg aaggaatgcg gacattgccg ccgttaagaa ccgcagggca ggcaaggttg
    10621 cggcaaaacg gctgaaagcg gcttccgact gtatgaagaa aggtgaaacg gacagattct
    10681 atgaggagat actgaaagcc atatggggtt atctgagcga taaactcagc attcctgttt
    10741 cggaccttac caggaacagt gccatggatg ccctgcagaa aagaggcatt gacgatgaaa
    10801 aaataaaaaa tctggcggac attctcgata agtgcgaata cgcgagatat gcacctgcct
    10861 cctcaggtac tgcagtgccg gagatttaca atgacgcatc aggatttatc aggtcagttg
    10921 aaaatgaaat ttaaacatga attttaaatc ttctctaata ccattaattc tgcttcttct
    10981 ttttgtgatt cctgatgcgg gggcacagga tccggccgct gaaaggttca acaggggaac
    11041 tgaatatttt tccgaaggca attatgaaaa ggcccttgaa gagtggaccg gtctgtatac
    11101 tgccggttac cgctcagcca ccctggatta caatattggc aatgcatatt tcaaacttgg
    11161 taatattccg ggatccatac tcttctatga aagggcccgc ctcaggaaac cggccgatga
    11221 gaacatcaga tacaatctcg gcattgccag ggccatgacc acggaccggc ttgatgaaat
    11281 ccctgaactt tttttcgtca ggtggtacga ttttctctcc ctcatccttt catcagacaa
    11341 ctgggcagta ataagccttg tttcctttat catatgcctt gccttccttt cactctattt
    11401 ctattcctca aaatacaaca tgaaagttgc cggattctgg cttgcagtcc tggcattgtt
    11461 aatttctctc gcttcgcttt ctttctccct gcggaataaa agactgattt atgaaagcaa
    11521 taaagccatt atcttcagtc ccatcgtcaa tgccaaaagc tcacccgacg acagcggaac
    11581 cgaccttttt gtgatacacg aagggctcag ggtgagtgtt gaggacgaag tcggtgagtg
    11641 gaaggaaata agacttcccg atggcaataa aggttgggtt ccggctaaca gtctcgaaat
    11701 tatctgataa aatgcaacat ttttgctgaa tttttcgtct gataattgtg atctgtaatt
    11761 cagtccgcaa atgagaaaaa atatctgcct tttatttgtt ttactggcat tgtctgcctg
    11821 taaaaaggaa aaatgggaac ctgaaggtcc cacggatgtc agaatcagaa atttacaaac
    11881 agatgaactt atggctgatg tggtgataaa caccgcaggg gtaagggata ctgtcgggaa
    11941 tgttaaaaaa ctaggaatgg tgaaacccgg acgaacatcg gaatacagga gggtatccat
    12001 tgcattcccg aaagctgaaa ttacggctga aatcaacggg gaaacatttt caaccggtcc
    12061 ggtcaacagt acctatatgc actacatagg gctgatgcgg attacctatg aggttcagat
    12121 aaaggatccg gaaaagaggc tgctcgaaat aaaaaacatc attccggatg aagccctgaa
    12181 gccctgaagc accgcagccc cggaattttg cagtactgaa atccggaagc acagaaactg
    12241 tcagacactg aagtgctgaa gcctgtagtt cgataatcct gaaccttttc gtcgataaaa
    12301 ctcagaaaat cacggatgaa gccggcctga accggtttat ctccagtcta ccggagcaat
    12361 accatgatcc gtaagatact tattgcagcg gctgaaatgc ctgtttccga aaaatcccct
    12421 tgaggcggaa aggggggaag gatgaacgga ttcaagaaca agatgcctgt tcctgtcaat
    12481 tgatgttccc ttcttctgtg cataggcgcc ccacagaaaa aaaacaagat tgtccctgtc
    12541 gttgtttaat acgctgatcg ctgcatccgt gaattcttcc cagcccttgc cctggtgcga
    12601 tgctgcctgg cgggctctca ccgtcagggt tgcatttaaa agaaacacac cctgggcggc
    12661 ccaacgcgaa agatttcccg aagaatatgg tttaattccc agatccgatt caatttcttt
    12721 gaaaatattg acaaggcttg gagggaaatc aattccttcc cttacggaaa aacacagtcc
    12781 gtgtgcctgt cccggaccgt ggtaaggatc ctggccaatg atcactactt taaccctgtc
    12841 gaagggacac agatcaaaag ccttgaatat caatccgccg ggtggaaaaa cctgagtctt
    12901 tccgtattct tcccttacaa agccggtaag acttataaaa taatccttac tgaactcatc
    12961 cttaagtttc tctttccagc tgctttctat ttttacgtcc atggtaagaa aaaaaatcac
    13021 agtctgcggg catagatttg ttttggatca ccgggtccgt aaaaatcctg caggctgacc
    13081 tcaagcctgt atccgttttt tttataaaat tcttcggtgg gtttatataa agggcggcct
    13141 gatgtttcaa tccagagtat ctttcctccc ttctgtctga ttctttcctc ggcagcctgt
    13201 aaaagagctc tgccataact gtggtttctg aaatcctgat gtactgcgat ccagtaaagt
    13261 tcccatgaat gaaccgagaa gtcatttttt ccgtaacatg caaagccgat taactgttcc
    13321 ccttcagtta ttctgatcca gaaatagccg cttgcatcca tgccttgcga aacagtctgg
    13381 acggctattt ccagtgcagt gcgtatttcg aaatcgtaaa aatatcctgt agacgccagt
    13441 atcttctcca gtcccggtat gtcttcatct cctatccctt cagatattct catatccgtt
    13501 tcagttattc agatcggtga ttatgcggcg gataacttca gaaaacttca gtcccgcaac
    13561 ccttgtggcg gccaccaggc cactgtcagg cgacaggcag ggatttgcat ttacctcaat
    13621 tacatagggt atgttgtttt tatcaacgcg caaatctatt cttgcatatc ccctgagacc
    13681 aaatatcttc cagcatttca gggcaatatc gcagatttgc ttttcaaggg ccttgtccag
    13741 cttatccccg gggaaatgtc tcagggtatt ctgaaattca aaggaatctt cttcccactt
    13801 agccttgaaa tcgacaatct tgggcttacc gtcccatgaa tcatgaaaaa tcatttctgc
    13861 tgcgggcatg acttccggtc ctgtttcacc cgaaataata ctgatgttga attccctccc
    13921 ctctatgtaa tcctgtatta gccagtgggt atcatcgtat tttttaagtt ttttgatgat
    13981 ttccggtgtg caggtgaaaa ccgaatcact tgttattccc agggagccgt cctcccatac
    14041 cggtttgatt atataggtgt tgccgggaat gagctggcct gactgggacg gagcgaatcc
    14101 tcccggatta tttattcctg cctgtttcag cgatctggca gtaaaagctt tgctggttgt
    14161 cacgaagatc gcttccacgg gattgcccgt atagggtatt gaataggcat tgaggagtgc
    14221 ggggatgaaa tagcataatt cccctttgcc gataatggat tccacaaggt tgaaaacgaa
    14281 atccactttg ccggcagcaa gtccggcaat ttctttcata atatcaacgg taatgcccgt
    14341 cctgaatgtc tcaaccccta tctcactgag attctt
//
LOCUS       DAOSPX010000042        14352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4293_length_14352_cov_3.697909, whole genome shotgun sequence.
ACCESSION   DAOSPX010000042 DAOSPX010000000
VERSION     DAOSPX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4293_length_14352_cov_3.697909"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1753
                     /gene="ftcD"
                     /locus_tag="PLZ75_03645"
     CDS             98..1753
                     /gene="ftcD"
                     /locus_tag="PLZ75_03645"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOS71525.1"
                     /translation="MTKRILECVPNFSEGRDMAIIKQITDEIESVENVKLLDVDPGRD
                     TNRTVVTFAGEPDAVAEAAFRAVKKASEVIDMSRHRGEHPRMGATDVCPFVPVSGISM
                     EETVIIARKVAERIGKELNIPVFCYENAAFRENRRSLAHCRSGEYEGLEKKFSDPEWK
                     PDFGPAVFNRKSGATAVGARDFLVAFNVNLNTTSTRRANAIAFDVRESGRDIINEKGE
                     KVNVPGSLRSVRAIGWYIKEYGIAQISMNLTDISVTPVHLAFDEVCRKAEARGVRVTG
                     SELVGLIPLRSLLEAGKYFLEKQKRSAGLPDEELIHIAVRSMGLDELQPFRPEEKIIE
                     YLLEKEKENLTSLSLKEYMDMTASELPVPGGGSASAYMGALGVALGTMVANLSAHRKD
                     WDERWKEFSVQAARGTEIQNMLLNLVDADTDAYNKIIEARRLPKSGDKERELRNAAIQ
                     EAYKQAILVPLKVMETAYSGFEIVKSMTETGNPSSVTDAAVGSLALLACIRGAHLNIK
                     INATGVEDSRFTGEILSRAGKIEEEAVHLQENILTAANRRISL"
     gene            2020..5133
                     /locus_tag="PLZ75_03650"
     CDS             2020..5133
                     /locus_tag="PLZ75_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS71526.1"
                     /translation="MKRILMFSTLLLITVTLVMAQTVQITGTVTGSEDGLPLPGVNVT
                     VKGTTIGSITGADGKYVLSLPVSAQTLVFSFIGFVTQEVPIQGRTDINVVLKQDLYNV
                     DEVVVVAYGTVQKRDITGSVSSVKADAIRNIPVQSFDQALQGKAAGVSITMPSGALNN
                     PPVIRIRGFNSITGSSSPLIVVDGVPIFSGDVSQTYSFANALSDINPSDIESMDILKD
                     ASATALYGSRAANGVIIITTKRGTGAKTNVTYDGYVGYTAPYHLFDLMNAQQYLSLKN
                     KALANSGSSTVLKYASGADGKNIDTDWADFVYQKGFQHNHAITFSGSTPSTSYFLSVG
                     YTNQEGMIRKSSFTRKNARLNIDHKLNKWVTLAANIAYTNGYNVSPNTGSDFSTAGAA
                     RLAFVLPSILGPYKADGSYNINGAAIGTMGTGLTGVSYYNPAAIIDLCKFTNETDRVL
                     ATLSGSLEPIKGLTLKTVYGIDNLSSESVEFRTPLTGDGYSDNGSVWNSFYRPKRWTW
                     TNTLNYNISLLEKFNFGFLAGAEEQYTKSVQWTASKYDVADPFFTVYQGSWVEPELGG
                     GVIGENYFISYFGRFNFNYNRKYYLEGSVRRDAFSGLSKDNKWGTFGGASVMWNASNE
                     QFISDAIGSVFSDIRLKASFGRVGNMSGIGNFSSLYLYGAGLYADVPTLAFSQIGNSA
                     LKWETSDKYDAGLSFGLLDDKIQAEINWYYNNVNGLILDVQQTPSKGIPGGTIPQNIG
                     SMFNTGFELTLTSYNFSTPRFSWTTTLNFSTLKNEVKALAPGIDYLVGTTGGLETTNR
                     TVVGEPIGNLFAVETRGVDPQSGRRIFVNKDGKEITYSHENPAATRWNYRDGSGVAPQ
                     ITLAGDGKIMGSPLPKYFGGLDNNLTYLGFDMTLNLTYAFDFDLYCGSKAGLRDQRFW
                     NNSVEVFETAWEKPGDKTNIPKPVWGDNVSNGSAIPISENIERGDFVKVRTVSAGYTF
                     KSLPAITKIEKIRIYAQLFNAFVFTKYSGSDPEVSTNRDANLTPGVDRNTAPQARTWT
                     FGLSVNF"
     gene            5152..6630
                     /locus_tag="PLZ75_03655"
     CDS             5152..6630
                     /locus_tag="PLZ75_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS71527.1"
                     /translation="MKNIYKFSIGLALLLLVGCSKEYLDPVPQTSLSSLTVFETKDRI
                     EAQVNGMYDAMKTAQFYGGRFFVYNDIRCDNFIPKSSNLVTGYSTWNHSVITSTNEVQ
                     NLWSAGYNTINTINIFLDGLKTAWDEGKLAGILTDTEYKQYTSEAYALRAISYFSLLQ
                     LYAKPFNMGNGNNPGLPLRLIAETSSENNDLARSSVAEVYTQILKDLNDAEGMAIANY
                     TTDQLNTTRIHKNSIIAYKTRVYLHMQDWAAVGTESAKIVALSGGKFSAPTGVAFELN
                     PSFTEIWKTPYTSKESIFSMPHSTTDNPGSQNSLPSYYFSGATESYYLNTAPGYAFSA
                     MDDADVRKTSLEKVNENYFISKFPDYTSHSNYSPVIRYAEVLLNRAEALVRAGNTVTA
                     EAVELLNAVRTRSFPAGAYQQADFADADAFYTAILKERNIEFLGEGIRNMDLMRLSLP
                     IPGKDGGSMGNISAVNPTDPTYIWPISALELTFNKLMTGNGK"
     gene            complement(6884..7660)
                     /locus_tag="PLZ75_03660"
     CDS             complement(6884..7660)
                     /locus_tag="PLZ75_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOS71528.1"
                     /translation="MDSYICENIFVPLRAGPSHKSEMLSQVLFGEKYRILDYSGHWIK
                     IETLFDSYAGWIDANHLQQKDDRPGSAGLVLNRSLLCFRKDKTRIVLEAGCEIYEPDF
                     DNKSFMLGNDRYTTCQEFTQGYVDVSASPADTAMKFINSPYIWGGRIPSGIDCSGFTQ
                     LVYKLCGITIPRDSRMQAETGETISFLEESIPGDLVFFDDESGRISHVGIVISRGLVI
                     HASGRVRVDTIDYHGIFKQELNTYSHRLRTIRRVVLSGNT"
     gene            7822..9219
                     /locus_tag="PLZ75_03665"
     CDS             7822..9219
                     /locus_tag="PLZ75_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOS71529.1"
                     /translation="MQKSADFAGKFPRFDKNKQHIAVFLGSIIAVVLTLTWGKNPGIK
                     EMAGTILVVTTCYIVLFFFLLQFRQEVLSVVLKTFFILLAILTFLIITRIVTGISARN
                     IIFLVPFAIIPVIIRTFYDARLALFILLITIMLAGFMVEEPFIFILMSFISGMVAIFT
                     LTNNFRKSRLLFTSLMVIISNMVLYSAILLMWKSSDLQFDPYDYMMLAGNGLLILLSY
                     PLILLFEQEFLLVSDTTLMELADTNQPLLRKLAVEAPGSFQHSLQVSNLAEEAARAIG
                     ANVNLVRAGGLYHDIGKIAKPTYFIENQSDGSSPHDKMDPRDSAQIIINHVKNGIVLA
                     KNYKIPAPVIDFIRTHHGTTIAYYFYKKFTDLHPEEGNRENLFAYPGPKPFSKETAIV
                     MMADAVEASSRSIQSYTEENISDLVERIIYLQEQDGQFANVPITYKDMSDIKSAFKKR
                     LTNIYHARIVYPERT"
     gene            complement(9216..9851)
                     /locus_tag="PLZ75_03670"
     CDS             complement(9216..9851)
                     /locus_tag="PLZ75_03670"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOS71530.1"
                     /translation="MKKMLYIFFLMVFPIAAAGQNDPKAVSILDNFSSAISAAPSVSM
                     KFLMITDDQMAKSSDTISGAILLSRDRYKLELQNNIIWYDGRDSWSYLPYEKEVTITR
                     PDKNDNSFESRPSSIFTMYKKGYKSRLVEEKRDSYLIDLYPEEIKSELIRVRMTIKKP
                     SLDLLSFEYKRRDGITITLLVQDYNLKQQTDPGMFRFNPEKYRDLEIIDMR"
     gene            complement(9863..12175)
                     /locus_tag="PLZ75_03675"
     CDS             complement(9863..12175)
                     /locus_tag="PLZ75_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOS71531.1"
                     /translation="MRFVSGILVSGIAIYLLLAFIAYLIWWKADQSIPTADILSGPDV
                     EVKNWSGKSGYYLSFLMISNGFGLGAFFIPMIFGTLGLYLLKFPKIRFWSLTAKFVFA
                     TIILSLILAFIFGKADGYLYAGPGGAHGYHFINWLNAFVGKPGTAIILILLTLSYLVF
                     ALKVPPSVFSRKKKEMSGQEDITGGGESGKYPAGKKDDGNTGFRINNRTGTDDQVFRD
                     EGVSVLSPATGSLIHDLDRDQPPDPGEKKAGMRITKTEVRDLVPEEELERIMKNYDPR
                     LDLSNYRFPPVSILKDHRSGSAFDNNEVIENKENIIKTLGDYKISIKHISATVGPTVT
                     LYEIIPERGVKLARIKSLEDDIALNLSAMGIRIIAPIPGRGTVGIEVPNKKPEIVSMR
                     SLIASRAFQESRFDIALALGRTITNDPYIVDLTKMPHLLVAGATGQGKSVGLNTIITS
                     ILYKKHPAELKFVLIDPKRVELPLYSKIERHYLAKLPGEEDAIITDTQKVINTLYSLC
                     IEMDNRLELLKAAQSRNIKEYNEKFITRRLNPEKGHRYLPYIILVIDEFADLIMTAGR
                     EIEGPLGRLAQVARAIGIHLIISTQRPTTNIITGFIKANFPTRIAFRVFSSIDSRTIL
                     DVTGADRLVGNGDMLISSGAEPVRVQCAYIDTSEIEELTEYIGSQQGYPDAMHLPEYI
                     NDTDGTASEVDMRKKDALFEDAARLVVQYQQGSTSLIQRKLSIGYNRAGRIIDQLEAA
                     GIVGPFEGSKAREVLCMDIMALEQILKNLE"
     gene            complement(12398..13090)
                     /locus_tag="PLZ75_03680"
     CDS             complement(12398..13090)
                     /locus_tag="PLZ75_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71532.1"
                     /translation="MRVSLLFLSVAIISVTGDSCVNSGVRNIDQGEIHYSITYSGDSG
                     FIPREFMPKHLIVCFKDNKILFDISAPFGNSGIMNLANPVNGIFDTYINFLGFKYFYP
                     SGPDENYPGFEAMTGMEIHKTSGTSVICGFNCKSAEVTFPGSKTGNKKYRIWYTDEIK
                     VRNPNAATPFNEIDGVLMKFFFVINQREMHFEAETVYRKDIPDKIFERKEKYVRISKK
                     DIDEIIDKFVSM"
     gene            13440..>14352
                     /gene="argF"
                     /locus_tag="PLZ75_03685"
     CDS             13440..>14352
                     /gene="argF"
                     /locus_tag="PLZ75_03685"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOS71533.1"
                     /translation="MEINLKNRHFLKLLDFTPEEINYLLDLAAKLKTEKQSGKEIQRL
                     KGKNIALIFEKTSTRTRCAFEVAAFDQGANITYLGPGGSQIGNKESMKDTARVLGRMF
                     HGIEYRGYGQKLVEDLAEYSGVPVWNGLTNEFHPTQVLADLMTMKEHSDRNLNNISLC
                     YLGDARFNMGNSLMAGSAKMGIDFRIAAPAMFQPSANLTGICRAVAEKTGARITITEN
                     LDEAVRGADFLYTDVWVSMGEPEDVWEERIRLLRPYQVNMEVIRKTGNPDVRFLHCLP
                     AFHNRETSIGQEIYEKYGLDGMEVTEDV"
BASE COUNT         4037 a         3048 c         3232 g         4035 t
ORIGIN      
        1 atgccctatg cctttggcag caacctggta gaaacagtga tattaaaagg aaaaagaatc
       61 atctgaacca gtgttaatta acaaataata cctctttatg acgaaaagaa tactggaatg
      121 tgttccgaat ttcagcgaag gccgtgatat ggccataata aagcaaataa cagacgaaat
      181 tgaatctgtt gagaatgtga aacttcttga tgtcgatccc ggaagagaca ccaaccgtac
      241 tgttgtgaca tttgcagggg agcctgacgc agttgccgaa gcagctttca gagctgtgaa
      301 aaaggcctcg gaagtgatag atatgtccag gcacaggggc gagcatccca gaatgggagc
      361 gaccgatgtt tgtccattcg taccggtatc agggatatcc atggaggaaa ctgtaataat
      421 agcccggaaa gtggctgaac gtataggcaa agaactaaac attcctgtgt tctgttacga
      481 aaatgctgca tttagggaaa acagacggag tcttgcccat tgcaggtcgg gagaatatga
      541 agggcttgag aagaagttct ctgatccgga atggaaaccc gatttcggac cggctgtctt
      601 caacaggaaa tcgggcgcca cggcagtcgg ggcacgggat ttcctggtcg ccttcaatgt
      661 aaatctcaac acgacatcga ccaggcgtgc caatgccata gcatttgacg taagggaaag
      721 cggcagggat attatcaatg aaaagggtga aaaagtgaat gttccgggct ctctcagaag
      781 tgtaagggct attggctggt atataaagga atacggaata gcacagatct caatgaatct
      841 gacagacatt tccgtaacac ccgttcatct tgcctttgat gaggtatgca gaaaagcaga
      901 ggcaagaggt gtcagggtta caggttcgga actggtggga cttattcccc tgagatcact
      961 gcttgaagca gggaaatatt tcctggaaaa gcaaaaaagg tccgcgggcc ttcctgacga
     1021 agagctgata catattgctg tcaggtcgat gggacttgat gagttgcagc ctttcaggcc
     1081 ggaggagaag ataattgaat atctgctgga gaaagagaaa gaaaatctca cttcccttag
     1141 tctgaaagaa tatatggata tgacagcttc cgagcttccg gttccgggtg gaggctccgc
     1201 atcggcttac atgggtgcac tgggagtggc actgggaaca atggttgcaa atctttcagc
     1261 ccacaggaag gactgggatg aacgctggaa ggaattttcc gtccaggccg cgcggggaac
     1321 ggaaattcag aacatgcttc taaatcttgt ggatgcagat acggatgcgt acaataaaat
     1381 aattgaagca cggaggctgc cgaaatcagg tgacaaagag agagagctgc gaaatgccgc
     1441 catccaggaa gcatacaaac aggctattct ggttccctta aaggttatgg aaaccgctta
     1501 ttccggattt gaaatagtaa aaagcatgac tgaaacaggc aatccttctt cggtaacaga
     1561 tgcggcggtt ggatcccttg ccctgctggc ctgtatcagg ggtgcccatc tcaatataaa
     1621 aatcaacgca acaggagttg aggattcccg ttttaccggt gaaattcttt ccagggcggg
     1681 aaagatcgag gaggaagctg ttcatctgca ggaaaatata ctgactgcag caaacaggag
     1741 gatatctctt tgaacagggg aaaggggaga agcatagcaa ttttctttta ctgatgaaag
     1801 agaaaatacc tctttgatac cgtaagtttt agcaattttc ggttttcatc tttcatacat
     1861 ttatgaaatt ccttctctgt tggtcaaaaa aaaggaagaa gatatgtttt atattaaatt
     1921 tttaaataaa ttcgtcatca gttttaacta ttaacctgga cccaagtttt aactattaac
     1981 ctaaactcaa actttaacta ttaacctaaa cttatgttta tgaagcgaat tctgatgttt
     2041 agcacactgt tgctgataac agttacactt gttatggctc aaacagtgca gattaccgga
     2101 acagtgaccg gctctgagga tgggctgccg ctgcccggtg ttaatgttac ggtaaaagga
     2161 accacaattg gctcaatcac aggggctgac ggtaaatatg ttctgtcact accggtcagt
     2221 gcacaaaccc ttgtattcag ctttattggt tttgtaaccc aggaagtgcc gattcaggga
     2281 agaacagata tcaatgttgt tctgaaacag gacctttata atgtcgatga agtcgtggtg
     2341 gtggcctatg gtactgtaca gaaacgggat ataaccggtt ctgtgtcatc ggttaaggct
     2401 gatgccatca gaaatatacc tgtacaaagt ttcgaccagg ctttacaggg caaagctgca
     2461 ggcgtatcca tcaccatgcc aagcggagcc cttaataatc ctcctgttat acgtatcagg
     2521 ggttttaatt ctataactgg gagctcttct ccgcttattg ttgttgatgg ggtgccaata
     2581 ttttcgggtg atgtatcaca aacctattca tttgccaacg ccctctccga cattaatccg
     2641 tccgatattg aatcgatgga tattctgaag gatgcatcag ctactgcctt gtatggatcc
     2701 agggcagcca atggtgtcat tataatcact acaaaacgtg gtaccggggc caagacaaat
     2761 gttacatacg acggatacgt ggggtacacg gcaccttatc atctttttga tctaatgaat
     2821 gctcagcagt atctttccct taagaacaaa gctctcgcaa attcgggatc atcaactgtt
     2881 cttaagtatg ctagcggtgc ggacggaaag aatatagata ccgactgggc tgactttgtt
     2941 tatcagaaag gatttcagca caaccatgca attacatttt caggatcaac tccttcaaca
     3001 agttatttcc tttcagttgg atacaccaat caggaaggaa tgatccgcaa aagttccttt
     3061 acaaggaaaa atgcaaggtt gaatattgac cataagctga ataaatgggt tactctggct
     3121 gcaaatatcg cttatacaaa tgggtataac gtatcaccca acacgggatc ggatttttcc
     3181 actgcaggag ctgcccgttt ggcatttgtt ctgccttcca tccttggccc ttataaggca
     3241 gatggttcat acaatataaa cggagcggca atcggcacta tgggtactgg tctgacgggt
     3301 gtttcatact ataatcctgc tgcaataata gatctttgta agtttaccaa tgaaacagac
     3361 agggtccttg caacactttc cggaagtctg gaacctataa aaggacttac attgaaaact
     3421 gtatatggta ttgataacct gtcgtctgag tcagttgaat tccgtacacc tctgacagga
     3481 gacggatatt ccgacaacgg tagtgtctgg aattcattct acaggccaaa aagatggaca
     3541 tggaccaata cgctgaacta taatatctca ctccttgaaa aatttaattt tggatttctt
     3601 gcaggagctg aagagcaata tacaaaaagt gttcaatgga cagcctcaaa atatgatgtt
     3661 gccgatccat ttttcacggt ttaccagggt tcatgggtgg aaccggaatt gggtggtgga
     3721 gtcataggag aaaattattt catttcatat ttcggcaggt ttaattttaa ttataacagg
     3781 aagtattatc ttgaaggaag tgtgcgtcgt gatgccttct caggactctc aaaagataat
     3841 aaatggggta catttggagg tgcgtcagtc atgtggaatg cttcaaatga gcaatttatc
     3901 agtgatgcga tcggatcagt attttcagat atacgcctta aagccagctt tggccgggtt
     3961 ggtaacatgt ctggtatcgg taatttcagc tctttgtacc tctatggagc aggactgtat
     4021 gctgatgttc ctacactggc tttttcacag ataggtaatt cagcacttaa gtgggaaaca
     4081 agcgacaaat atgatgcagg tctgagcttc ggacttctgg atgataagat ccaggcagaa
     4141 attaactggt attacaataa tgtaaacggt cttatcctgg atgttcagca gacaccttca
     4201 aagggtattc cggggggaac aattcctcaa aacataggtt caatgttcaa taccggtttt
     4261 gaactaacac ttacctcata taatttctcg actccgcgat tttcctggac gactaccctg
     4321 aatttcagta ctttgaaaaa tgaagtaaaa gctcttgctc cgggtattga ttatcttgta
     4381 ggtaccacag gaggactgga aacgacaaac aggactgttg tcggagagcc gataggtaat
     4441 ctctttgctg ttgaaaccag aggtgttgat cctcagagtg gaagaagaat atttgtgaac
     4501 aaggatggaa aggaaattac ttattcccat gagaatccag ccgcaacaag atggaattac
     4561 agggacggtt ccggagttgc acctcagata acactggctg gtgatggcaa gataatgggg
     4621 tctcctctgc ccaaatattt tggaggattg gacaataatc tcacttatct tggttttgat
     4681 atgactctta atcttactta tgcatttgat tttgaccttt attgtggatc aaaagcaggt
     4741 ctgagagacc aaagattctg gaacaattca gttgaagtat ttgaaactgc atgggaaaaa
     4801 ccgggtgata aaacaaatat cccgaaaccg gtgtggggtg ataatgtatc gaatggatca
     4861 gccataccga tatccgagaa tattgaaaga ggtgatttcg tgaaagtaag aactgtctct
     4921 gcagggtata cattcaaatc cctgccagct ataacaaaga tcgaaaagat cagaatatac
     4981 gcgcagttgt tcaatgcatt cgtgtttacc aaatattccg gttccgaccc tgaagtatcc
     5041 accaaccgtg atgctaacct tacccccggt gtagacagga acacagcacc gcaggcaaga
     5101 acatggacgt ttggtttaag cgttaatttt taatttgtta aaaatactga tatgaaaaac
     5161 atatataaat tttcaatagg cctcgctctt ctgcttttag ttggatgttc aaaggaatat
     5221 cttgatccgg ttccccagac atcattgtca agccttactg tgtttgaaac aaaggataga
     5281 attgaggctc aggttaacgg aatgtatgat gcaatgaaaa ccgcccagtt ctacggagga
     5341 agattcttcg tatataatga tataagatgc gataatttca ttccgaaaag ttccaacctg
     5401 gtaacaggct attccacatg gaaccatagc gtaattacat caacaaatga agtccagaat
     5461 ctctggtctg ccggttataa tactattaat accataaata ttttccttga cggcctgaaa
     5521 acggcgtggg atgagggaaa attggcagga atactaactg ataccgaata taagcaatac
     5581 acaagtgagg catatgcact cagggccatt tcttatttta gcctccttca gttatatgct
     5641 aaacccttta acatgggcaa tggtaataat ccaggattgc cgctcagact gatcgcagaa
     5701 acctcttccg aaaataatga tctggccagg agttctgtgg cagaagttta tactcagata
     5761 cttaaggatc ttaatgatgc tgaaggaatg gctattgcaa attataccac agaccagctt
     5821 aataccacga gaattcataa aaattcaatt attgcttaca agaccagggt atatcttcac
     5881 atgcaagact gggccgcagt tgggacagaa tcagcaaaaa tagttgcttt atcggggggc
     5941 aagttctctg caccgacggg agtggcattt gaactcaatc cttcgtttac agaaatatgg
     6001 aaaaccccat atacctcgaa agaatctatt ttttcaatgc ctcattcaac cactgacaat
     6061 cctggctcac agaacagcct tccaagctat tatttctcag gtgcaacgga atcatattac
     6121 ctgaatacag ctccggggta tgcttttagt gcaatggatg acgctgatgt tagaaaaacg
     6181 agccttgaga aagtaaatga gaattatttt atttcgaaat ttcctgatta tacatcccat
     6241 tcaaattatt caccggtgat aaggtacgca gaagtacttc ttaaccgtgc agaagccctg
     6301 gtaagagcag gaaacactgt tactgccgaa gccgttgagc tccttaatgc tgtcagaacc
     6361 agatcgtttc ctgccggagc ctatcagcag gcagattttg cagatgctga tgctttctat
     6421 actgcaattc tgaaggaaag aaatatcgaa ttccttggtg aagggatccg taatatggac
     6481 cttatgagac tgagcctgcc tattccgggg aaagatggag gaagtatggg aaatatttca
     6541 gctgttaatc cgacagatcc tacatatata tggccaatct ctgctcttga actcaccttt
     6601 aacaaactga tgaccggaaa cggtaaatag aaaaaaaata gtttataaaa aaagttgtct
     6661 gccaatcggt aagacaactt tttttgttta tgcgggatac gggcgatgac tccggggaga
     6721 tagttgcgga tatacacatg aagtgaggct aagatggaaa agcctgttga acagttgaaa
     6781 tcaagaaaca gctgatgtgg aaatgaaaat cagaaggaag cgttaaatca ataagattat
     6841 gctatgcagg ccgtagcttc agcgtgggct tgcggcagtg cggttatgta ttaccgctta
     6901 ataccaccct gcgtatcgtc ctcaaacgat gcgagtacgt attaagctcc tgcttgaata
     6961 tcccatggta atctattgta tccaccctga ccctgcccga ggcatgaatg acaagtccgc
     7021 gcgatattac tatcccgaca tggctgattc ggcctgattc gtcatcgaaa aacaccaggt
     7081 cacccggaat gctttcttcg agaaagctga ttgtttctcc tgtttccgcc tgcatccggc
     7141 tgtcgcgcgg tattgtgatg ccgcaaagct tatagaccag ttgcgtgaat cccgagcaat
     7201 caatgcctga aggtatgcgg cctccccata tatagggact gttgatgaat ttcattgcag
     7261 tatccgcagg cgaagcagaa acatcaacat agccctgagt gaattcctgg caggtggtat
     7321 agcggtcatt cccgagcata aacgacttgt tgtcaaaatc cggttcatat atttcacagc
     7381 ctgcctccag tacaatcctt gtcttatcct tcctgaaaca caggagggat ctgttaagaa
     7441 ccagtccggc tgaaccaggc ctgtcatcct tctgctgaag atggttggcg tcaatccagc
     7501 ccgcatagct gtcaaagagg gtttctatct ttatccagtg acctgaatag tcaagtatgc
     7561 ggtatttttc cccgaacagt acctggctga gcatttcaga tttgtgtgag ggcccggcgc
     7621 gtaaaggcac aaagatattt tcacagatat agctgtccat ggcttaatca ttacataccc
     7681 aaaagtaata aattaaacat tatgtaaatt tctgccatct gctgtcagag atccggttcc
     7741 cgttaaactc cggctattct ttttaaaacc ggagtcttga caaaatttgc taattttacc
     7801 ccgcaaaaaa tgacaggatc aatgcaaaaa tctgcagatt ttgccggtaa atttcccagg
     7861 tttgataaaa acaaacagca tattgcagtt tttctgggca gtataatagc cgttgtacta
     7921 acacttacct ggggaaagaa tcccggtata aaagagatgg caggaaccat cctggttgtt
     7981 acaacctgct atatagtact ctttttcttc cttcttcagt tcagacagga agtgttgagt
     8041 gtcgttctga aaacattctt catacttctt gccatactga ccttcctgat cattacccgg
     8101 attgttaccg ggatctctgc ccggaatata atatttctcg ttccctttgc cataattcct
     8161 gttatcatac ggacattcta tgatgcgcgc cttgctctgt tcattcttct tattaccatc
     8221 atgctggcag gtttcatggt ggaggagccc ttcatcttca tattgatgtc cttcatttcc
     8281 ggaatggttg caatattcac actgacgaac aacttcagaa agtcgcggct cctgtttact
     8341 tccctgatgg tgattatcag caatatggtg ttatattccg ccatacttct catgtggaaa
     8401 tcctctgatc tccagtttga tccatatgac tacatgatgc ttgccgggaa cggccttctt
     8461 atccttctga gctatcccct gatattgctt tttgaacagg aattcctgct ggtgtcagat
     8521 accacactca tggaacttgc cgacacgaac cagccacttt taagaaaact tgcggttgag
     8581 gctcccgggt catttcagca ttccctgcag gtttcaaatc ttgccgagga agctgcgagg
     8641 gcaataggag caaatgtaaa cctcgtacgt gccggcgggc tttatcatga tatcggcaaa
     8701 attgcaaaac ccacctattt tatcgagaat cagtcagacg gatcaagtcc gcatgataaa
     8761 atggatccaa gggacagtgc acagataatc atcaaccatg taaagaacgg catagtcctt
     8821 gccaagaact ataagattcc cgctccggtt attgatttca tcaggactca tcacgggacg
     8881 accattgcat attatttcta caaaaaattc accgatctgc atcccgaaga gggcaacagg
     8941 gagaatctct ttgcctatcc cgggccaaag cccttttcca aggaaacagc aattgttatg
     9001 atggctgatg ccgttgaggc atcttcaaga agtatccagt cctatacgga ggaaaatatc
     9061 agtgacctgg ttgaaaggat aatctatctt caggaacagg atggccagtt tgcgaatgtg
     9121 ccgataacct acaaggatat gtcggatatt aaaagtgcct tcaaaaaaag gctgaccaat
     9181 atttatcatg caaggatagt ttatcctgag aggacctagc gcatatctat aatctccaga
     9241 tccctgtatt tctcaggatt gaatctgaac atcccgggat ctgtctgctg cttcagattg
     9301 taatcctgta ccagcagggt aattgttatt ccgtcccttc gcttgtattc aaagctcagc
     9361 aggtcaagtg atggcttttt aattgtcatc ctgaccctta tcagttcgct ttttatctct
     9421 tccggataaa gatctatcag gtatgaatcc cttttctctt caaccagcct ggatttatat
     9481 ccctttttat acattgtaaa gatggatgag ggacggcttt cgaaggagtt gtcgttttta
     9541 tccggccggg tgattgttac ttccttttca taaggcaggt aactccagga atccctgcca
     9601 tcataccaga taatattatt ctgaagttca agcttgtatc tgtccctgct cagtaaaatc
     9661 gccccgctta tggtatcgct tgattttgcc atctgatcgt cggtgatcat gaggaatttc
     9721 atacttaccg aaggagcggc gctaatggct gatgagaaat tatcaagtat gctcaccgct
     9781 ttcggatcat tttgtcctgc cgctgcaatt gggaaaacca tcagaaaaaa tatgtaaagc
     9841 atctttttca ttgacggaag atttattcaa ggttcttcaa aatctgttcc aaagccatta
     9901 tgtccatgca caatacttcc cttgccttgc ttccctcaaa tggcccgact atacccgctg
     9961 cctcaagctg gtcaatgatc ctgccggccc tgttgtatcc tatggagagt ttcctctgaa
    10021 taagggaggt tgacccctgc tgatactgaa ccaccagtcg tgccgcatcc tcaaacagag
    10081 catccttttt cctcatgtcc acctcggagg cagttccgtc ggtatcgttg atatattccg
    10141 gaaggtgcat tgcatcggga tagccctgct gtgatccgat gtattctgta agttcctcta
    10201 tttcgctggt gtctatatat gcgcactgga cgcgtaccgg ttcagcaccc gaggagataa
    10261 gcatgtctcc gttgcctaca agccggtccg caccggtgac gtcaagtatt gtccttgaat
    10321 caattgatga aaatacacgg aaggctatcc ttgtaggaaa attggctttg atgaaacctg
    10381 taatgatatt ggtggtggga cgctgggtgg aaattatgag atgaatccct atggccctgg
    10441 caacctgtgc cagccttccg aggggtccct ctatctccct gcctgctgtc atgatgagat
    10501 cggcaaactc atcgataacc aggatgatgt aagggagata cctgtgtccc ttttcgggat
    10561 taagccttct tgttatgaat ttttcattgt attcctttat attcctcgat tgtgcggctt
    10621 tcaggagttc aaggcggtta tccatttcaa tacacaggga atacagtgtg ttgataactt
    10681 tctgggtatc cgttatgatg gcgtcttcct cgccggggag cttggccagg taatgccgtt
    10741 cgatctttga atacaatggc agttcaaccc ttttcggatc aatgaggaca aacttgagtt
    10801 cggcaggatg ttttttgtac aatatggatg tgataatagt attgagtccg accgattttc
    10861 cctggccggt tgctcctgca acaagaaggt gaggcatctt ggtcaggtct acaatgtagg
    10921 ggtcattcgt tattgtcctt ccgagtgcaa gtgcaatgtc gaacctggat tcctgaaagg
    10981 ccctggaagc aataagtgat cgcattgaca ctatttccgg tttcttgttg ggcacttcaa
    11041 tgccaactgt ccccctgccc ggtatcggtg caatgattct gatacccatg gctgaaaggt
    11101 tcagtgcaat gtcatcctca agcgatttga tgcgtgccag cttgacacct ctctccggaa
    11161 taatttcata aagtgtgacc gtcgggccga ctgtcgctga aatatgcttt atgcttattt
    11221 tgtagtcacc gagtgtcttt attatgtttt ccttgttctc aatcacctcg ttgttatcga
    11281 aggcactgcc cgacctgtga tccttcagga ttgaaacggg cggaaacctg tagttcgaaa
    11341 gatcaaggcg gggatcatag tttttcatga tccgttccag ttcttcttcg ggaacaaggt
    11401 ccctcacttc cgtttttgtt atccgcatac ccgctttctt ctcaccggga tccggaggct
    11461 gatccctgtc cagatcgtgg ataagtgaac ctgttgcagg agacaggact gatacgcctt
    11521 catcgcggaa tacctgatca tcagttcctg tccggttatt tatgcggaat cctgtattgc
    11581 cgtcatcctt ttttccggcc gggtattttc cgctttcgcc tcctcctgtt atatcctcct
    11641 gtccggacat ttccttcttt tttctgctga aaactgaagg tggtactttc agagcaaaaa
    11701 ccagatagct cagtgtcaga aggatcagaa tgatagctgt tcccggcttt cctacaaatg
    11761 cattcagcca gtttataaaa tgatatccgt gtgccccgcc cgggcctgca taaagatatc
    11821 cgtctgcctt cccgaaaatg aaagcaagaa taagggacag aattatcgtt gcaaagacaa
    11881 attttgctgt aaggctccag aatcttattt tcgggaattt aagcaggtat aatcccaggg
    11941 ttccgaaaat catgggaata aaaaaagcac ccagaccaaa tccgttgctt atcatcaaaa
    12001 aggaaagata atacccgctt ttccccgacc agttctttac ttccacatcc gggccggata
    12061 atatatctgc cgtgggaatg ctctggtccg ctttccacca tatgagatag gctatgaaag
    12121 ccaggagcag gtagatggca atgccgctga caaggatacc gcttacaaac ctcattcgtt
    12181 catccttgaa aaacgctttc ttttttccct tgtttcttac ggttttatca gattttttag
    12241 acacgttttt tatccttgtc cggtttatgc attaataaat gcaaggcaaa gttagtaatt
    12301 attcgaaatt cagaaataca aatgtgaagt gcagtccggg atggcagggc aaaacacctg
    12361 ctccggaggt gagaaaaaat aaaatccggc cggaaaatta catgctgacg aatttgtcaa
    12421 taatttcatc tatatccttc tttgagatcc ggacatactt ttccttcctt tcaaatatct
    12481 tgtcgggaat atcctttctg tataccgtct cggcctcaaa atgcatttcc ctctggttta
    12541 ttacaaaaaa gaatttcatc agtaccccgt ctatttcatt gaaaggagtt gccgcattcg
    12601 ggtttctgac ctttatttca tcggtgtacc agatgcggta ttttttgttg ccggttttac
    12661 tgcccggaaa cgtgacttca gctgatttgc agttaaatcc gcaaatcacc gaagtgccgg
    12721 aggttttatg tatctccatc cccgtcatgg cctcaaatcc gggatagttc tcatcagggc
    12781 ctgaaggata gaaatattta aaacccagaa aatttatgta tgtatcgaat attccgttta
    12841 cgggattggc cagattcata attcccgagt ttccaaaagg agccgatata tcaaaaagaa
    12901 ttttattatc tttaaaacag actatcagat gcttaggcat aaattccctg ggaatgaacc
    12961 cggaatcacc gctataggtg attgaataat gaatctcacc ctggtctatg ttcctgactc
    13021 cggaattaac acaggaatct cctgttacag atataattgc gacagataaa aacagcaggc
    13081 tgactctcac ttattttttg ttttgacatg taaaaatagc ttttttttat tagaaatcct
    13141 ctatctttca aatgattgtt gtttccgtta ttttaacggc cgctgctgaa acatgtcctg
    13201 cgtgggttta tgttgtttgt attcagggat atcgggtgcc ggttgaaaca tctgaaaaag
    13261 gaccggtccg gcagaaattc agaaattttt gtccggtgat ttttccgcag caggcagttt
    13321 ggtttaataa atttataaat ttgcctgatt cagtttcaag tataataatc ctcaaagata
    13381 cttgtactgc tttactcagg ggtccctgcc ggaatgcagg gaacataaac atctgaacaa
    13441 tggaaataaa cctcaaaaac cgtcattttc tcaaacttct tgactttact ccggaagaga
    13501 taaattatct gctggatctt gcagcaaagc tgaaaactga aaaacaatcg gggaaggaga
    13561 ttcagcggct gaaagggaaa aatattgctc tcatttttga gaaaacctct acccggacga
    13621 ggtgtgcatt tgaagttgca gcctttgatc agggtgcgaa tattacctat ctgggcccgg
    13681 gcggctcaca gatcggaaac aaggaatcta tgaaagacac agccagggtg ctgggaagaa
    13741 tgtttcacgg gatagaatac cggggatacg gacagaaact cgttgaggat ctggctgaat
    13801 attcaggagt tcctgtctgg aacggactta cgaacgagtt tcaccctact caggtccttg
    13861 cggatttgat gacaatgaag gaacattcgg acaggaatct gaacaatatt tcattatgct
    13921 atctcggcga tgcccgtttc aatatgggta attcgctcat ggcagggtcg gcaaaaatgg
    13981 gaattgattt cagaattgct gcaccggcta tgtttcagcc ctccgccaat ctgaccggga
    14041 tttgcagggc agtggctgag aagacaggag caaggatcac catcacggaa aacctggacg
    14101 aagcagtcag gggagctgat tttctttata ccgatgtatg ggtctcaatg ggtgaaccgg
    14161 aagatgtatg ggaagaaaga ataaggcttc tcaggcctta ccaggtgaat atggaagtga
    14221 tcagaaaaac cggaaatccg gatgtcaggt ttcttcactg tcttccggca tttcataaca
    14281 gggaaacatc aataggtcag gagatatatg aaaaatacgg cctcgacgga atggaggtga
    14341 ctgaagatgt tt
//
LOCUS       DAOSPX010000043        14293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4317_length_14293_cov_4.313457, whole genome shotgun sequence.
ACCESSION   DAOSPX010000043 DAOSPX010000000
VERSION     DAOSPX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14293
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4317_length_14293_cov_4.313457"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLZ75_03690"
     CDS             <1..819
                     /locus_tag="PLZ75_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOS71534.1"
                     /translation="ALIAEMGKKETESFGPVISELSRSYRIRSRIQVTISDLGGNPVH
                     GIFRVCGVYRTANTGFDQTTAFVNEDELVKLYGGKEILTHEIAILLNNTDESGMVRDK
                     LNRIAGNNSVSTWRELAPDAALLNDFMIVYYFIFIGIIMLALAFGIINTMLMAVLERT
                     RELGMLMAIGMNRRRVFAMIMLETVFLTFTGAIAGMLSGWVITGILGKTGIYLSGWAE
                     GFEALGFASRVYPVVTIDFIMFTSLMVTATAILASVWPARKALKLIPADALSTK"
     gene            844..1530
                     /locus_tag="PLZ75_03695"
     CDS             844..1530
                     /locus_tag="PLZ75_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS71535.1"
                     /translation="MKVLELKNVNKIYNSAEIKVHAVNDVTLEFEEAEFAAIVGPSGS
                     GKTTLLNMIGGLDMPTSGEIIIGGVDLSRLRPSELIDFRLNNIGFVFQSFNLIPVLTA
                     KENVEFIMALQKWPADKREERTLELLRAVGLEERINSRPSKLSGGQQQRVAVARALAS
                     RPKFVLADEPTANLDSKAAAILLEIMEKLNRDEKITFIFSTHDPRVMKMARRVITLED
                     GKVISDEGKE"
     gene            1545..2699
                     /locus_tag="PLZ75_03700"
     CDS             1545..2699
                     /locus_tag="PLZ75_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71536.1"
                     /translation="MTRIKILLQLSLLFISVPAFNQEDGKKIAISGYLSSVQSVMFDT
                     LKGPFVNDYILHNRLNFKSWLNDHITFAAEFRNRLFTGDMAKAGKTYAVLTGSDDGFV
                     DMSWNLISGKSFFFNTTIDRLWVDISYEKFQVCAGRQRINWGQALVWNPNDIFNTYSF
                     FDVDYIERPGCDAVRLQYYPSWSSALELAVSAGSEKDITAAGLFRFSRWGYDIQFLAG
                     YSNSSDIVAGAGWSGPAGSFSFRGETTWFRPAESFLNSRGTILVTAGVDRIFSDNSVV
                     QLQMMYCNDPQGPGTLTKLLASGLSARNLAFSKFTLFGQCVLAATPLLNTGLSSMWFP
                     DLKGFFAGPSLEWSMAQNVDFSFIWQHFNGKTDELRSRINLMFLKFRYSF"
     gene            2736..3473
                     /locus_tag="PLZ75_03705"
     CDS             2736..3473
                     /locus_tag="PLZ75_03705"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOS71537.1"
                     /translation="MALVHEFERSGNWLFRRRSWLPLFLVAAGILILWGGNRQAGSFN
                     LRDEMVFLAISLTGEIIRILTVGFAPQGTSGRNTSAGQVAWELNETGAYSLVRHPLYA
                     GNFLMWLGPVLFLRSAWFTVIFILVYWLYYERIMFAEEQFLRRKFGEKYDEYSSKVYP
                     IIPFKFNFLPPALPFSLRNVLKREYHSFVNIFLIFAALDISRNYFLTGRLFITDIWIW
                     LTCGAGIIWLIIRLIHKFTDWLEVEGR"
     gene            complement(3498..4175)
                     /locus_tag="PLZ75_03710"
     CDS             complement(3498..4175)
                     /locus_tag="PLZ75_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOS71538.1"
                     /translation="MNIWLIFIAVMVLSWIASSTLKSKFRKYSKIPVENGMTGKEIAE
                     KMLHDNGIYDVSVVSVPGQLTDHYNPEKKTINLSRDVYASRSIAAAAVAAHETGHAVQ
                     HANAYAWLTLRSRLVPVVSFSSKWVTWVILAGILMIKTFPALLFTGIVLFALTTLFSF
                     ITLPVEFNASQRALVWLSGTGVTSHENQARARDALKWAAMTYVIAAVGSLATLLYYIM
                     IFTGARE"
     gene            4329..4559
                     /gene="yidD"
                     /locus_tag="PLZ75_03715"
                     /pseudo
     CDS             4329..4559
                     /gene="yidD"
                     /locus_tag="PLZ75_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
     gene            complement(4683..5435)
                     /locus_tag="PLZ75_03720"
     CDS             complement(4683..5435)
                     /locus_tag="PLZ75_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOS71539.1"
                     /translation="MKKIRTIIIDDESPARELIRHYLQDLDYIDIISECSDGFSGLKA
                     ITAMKPGLVFLDIQMPRLTGIEMLEVMTEKPEIIFTTAYDQYAIRAFELNAVDYLLKP
                     FDKRRFLDAVNRAAEKIRSGTAGREAANRILSQLPAPEAPVARIVVRTGNAIRIIPVE
                     KIRYLEAQDDYVMVHHIEGRALKQQTMKYYEDNLPPADFIRIHRSYMVRVQEISKIEP
                     YGKDSHIAVLKSGDRLPVSRTGYQNLKKELDF"
     gene            complement(5586..6638)
                     /locus_tag="PLZ75_03725"
     CDS             complement(5586..6638)
                     /locus_tag="PLZ75_03725"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOS71540.1"
                     /translation="MKHPVLQNRTRLIIWWLVWIILAAGQILLFYFIYGYFDALSIPD
                     TLLSFFILSVLILSLWYPFSYINRENTKVFLIVFSLIIAGTVFISLSMVITRMTLQSV
                     TGDKLFFGIYWKSTLPYRAGASVFIYILAVVTYYLFISLGKLSEKKAREAKLESLLKE
                     TELKMLRSQINPHFLFNSLNSVSSLTLTNPEKAREMVIKLSEFIRYGLSKKDEQAVSL
                     RNELENLKLYSDIEKIRFGDRLSTEENIDTQCLDLKLPVMILQPLFENAIKHGVYESS
                     GSVTIKTEVKKTEDCVQITISNNFESSPSFRKGTGTGLANVENRLSLFYGNKARLITS
                     KEKDVFSVTLFIPHET"
     gene            complement(6655..7404)
                     /locus_tag="PLZ75_03730"
     CDS             complement(6655..7404)
                     /locus_tag="PLZ75_03730"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOS71541.1"
                     /translation="METNDEYRKHPGYQGREHVYDKNNKATVGVILVLTGLFLLLRNT
                     GIFPDFIDRVIFSWQMLLVVIGLVMTVNSREKSAGIIVMAVGGFFMIPLVFRHTFHMY
                     NLFWPAIFIIAGILFISSRRKGWSGRVTKDLTSDDYIDYINIFSGGDRQIVSNNFRGG
                     KITAVFGGTELNLTKAVLAPGRNEIEIICVFGGATIIVPESWQVTIEVTPVLGGFTDS
                     RKLRQERPVNPESQLVIKGTVVFGGGEVKSY"
     gene            complement(7415..7795)
                     /locus_tag="PLZ75_03735"
     CDS             complement(7415..7795)
                     /locus_tag="PLZ75_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOS71542.1"
                     /translation="MKKRDTFVQKQREHHPGRGIGLFLIVLGLALLVATNDLLHLGGI
                     KEYFTWETAMIFVGVLLILNPSLLPGILLIAGGVWFLYENRLGGMPAAVKVFYWPSVV
                     ILAGISLIIASFFRKKKQAEKTNI"
     gene            7971..8489
                     /locus_tag="PLZ75_03740"
     CDS             7971..8489
                     /locus_tag="PLZ75_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOS71543.1"
                     /translation="MSGLYSIPISGMKEGCRSFDFEIGNDLFAHFEEAEVREGSLKAI
                     VQAEKGPAHIDLTILIDGAVNISCDRCLGIFPFPVHCINSLLVKFGKTHDESDPDIIL
                     LSEEENELDLMQYFYEYIILALPIQRIHPADESGNSTCDPEMISRLNRHLKNNNTGTD
                     PRWDELRKITNN"
     gene            8501..8686
                     /gene="rpmF"
                     /locus_tag="PLZ75_03745"
     CDS             8501..8686
                     /gene="rpmF"
                     /locus_tag="PLZ75_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOS71544.1"
                     /translation="MAHPKRKQSKARRDKRRTHDKATAPTVISCSNCGFSVQYHRVCP
                     ECGYYRGKPAVEKKTSA"
     gene            8712..9650
                     /locus_tag="PLZ75_03750"
     CDS             8712..9650
                     /locus_tag="PLZ75_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HOS71545.1"
                     /translation="MRIGLDIMGGDFAPGAVIEGAIDTLVHLSGEDKLVLIGDQQAVE
                     SKLRESTADLSRVEIVHSTQVIGMGDIPSKAYSHKPDSSIAVGYRLLKEGRIDGFCSA
                     GNTGAMLVGASYTVNVIPGVLRPALAAIVPLFDDRNAVILDVGLNPDTKPDVLLQYGL
                     LGSFFAKYVLKTDSPVVKLLNIGEEESKGTAAIRAAYELLKSHPGINFQGNFEANEMF
                     RENNPDVLVCDGFVGNIVLKQTEAFYRLYKSRNLGDPYFERLNFENIGGTPVVGINAN
                     VVIGHGISKRKAIMNMILQTLAVVRVNLSQKIKESI"
     gene            9653..10651
                     /locus_tag="PLZ75_03755"
     CDS             9653..10651
                     /locus_tag="PLZ75_03755"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOS71546.1"
                     /translation="MGKIRAAITGIHAWVPEYRLTNFELSRMVDTSDEWIMQRVGIKE
                     RRILKGEGLGTSELGVNAVKGLLEKTGTSPAEVELLICATITPDMMFPATANIISDKA
                     GIKNAFSFDLSAACSGFLFALQTGAAYIESGLCRKVVIVGADKMSSITDYTDRTTCPL
                     FGDGAGAVLLEPTEEEVGIMDHILNVDGSGCRYLHMKAGGSARPASHETVEAREHFVY
                     QEGQAVFKFAVSSMADVAAEIMVRNNLKPEDIAYLVPHQANLRIIDATGRRMGLPPEK
                     VMVNIENYGNTTAATIPLCLWDYEKKLKKGDILILAAFGGGFTWGSIYLKWAYDPQ"
     gene            10742..11137
                     /locus_tag="PLZ75_03760"
     CDS             10742..11137
                     /locus_tag="PLZ75_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOS71547.1"
                     /translation="MAKFNEAEIAAINLISSGTDITGDIKSGGDLRIDGTLNGNLNTK
                     GKVVIGPTGKVNGEVVCKNSEVSGIVEGKITVHQLLILKVSSKILGDIVTAKLSIEPG
                     AIFSGNCKMSDNETGGGAGKGKESEKTSR"
     gene            11082..11327
                     /locus_tag="PLZ75_03765"
     CDS             11082..11327
                     /locus_tag="PLZ75_03765"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOS71548.1"
                     /translation="MKPEEELEKEKNQKKPQDKRLSSYAKYTGMAFQMGIIILATTWG
                     GIKIDQLAGFEKPVFTIILSLLGVFAAIYTAVKDFLK"
     gene            11336..11722
                     /locus_tag="PLZ75_03770"
     CDS             11336..11722
                     /locus_tag="PLZ75_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71549.1"
                     /translation="MKLFLKNITGLVILTVIVLSAGLILVLKYGPGFNFGELALLTLT
                     FAILALVSMTIIHKGRKKDADAQVIHLLVAVGIKFITDLVIALVWFFVLKKTSVTLVI
                     LFFVLYLAFTLFSVLGVLKTLKYKPL"
     gene            11741..12826
                     /gene="atpB"
                     /locus_tag="PLZ75_03775"
     CDS             11741..12826
                     /gene="atpB"
                     /locus_tag="PLZ75_03775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOS71550.1"
                     /translation="MRIVTFVFLILLHIAYPVSGSVQNGSGPHEEKNQTEAGTKFNAS
                     EFIIDHISDSHEWHIMTLGEGENEKHITLYLPVILYSKKSGLHLFSSRRLAHGHEYQG
                     FRLMQEGDLKGKIVSLSENGEVDSENLLLDFSMTKTVVGMLAAAIIGLTLFLLLSRSY
                     SKTGISHPKGIQSLLEPVICFVRDDIAIPNIGHEKHARFMPYLLSVFFFILINNIMGL
                     IPFPPPFGANVTGNIAVTFTLAFFSFVIIQINGSKSYWRHIFAAPGLPVWMLPLMIPV
                     EMIGVIAKPFALMVRLFANITAGHIIVMSLLALIFIFNSLFVAPVTILFVIFMDCLEL
                     LVAFLQAYVFTLLSALFISLAVVEEHH"
     gene            12858..13061
                     /gene="atpE"
                     /locus_tag="PLZ75_03780"
     CDS             12858..13061
                     /gene="atpE"
                     /locus_tag="PLZ75_03780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOS71551.1"
                     /translation="MTGTLAAIGAGMAVIGASYGIGWIGKSAMEAIARQPEAANKVQT
                     AMIIAAALIEGVALFAVVVALIA"
     gene            13128..13643
                     /gene="atpF"
                     /locus_tag="PLZ75_03785"
     CDS             13128..13643
                     /gene="atpF"
                     /locus_tag="PLZ75_03785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOS71552.1"
                     /translation="MILASNSLTSPAVGTIFWAVLIFGIFFYILSKFAWKPILSALKQ
                     RDDLIKGSLEAAENARREMIQLQSDNEAILQKAREERENILKEARDVRDKLISEAKVR
                     ATEEASKIVESAKTGIERERNKVLSEIHDQLATLSVDIASKLIGEHLSGSGEQEKLIR
                     KYLDDIKINKN"
     gene            13643..14185
                     /gene="atpH"
                     /locus_tag="PLZ75_03790"
     CDS             13643..14185
                     /gene="atpH"
                     /locus_tag="PLZ75_03790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOS71553.1"
                     /translation="MNDGKISVRYARALFLSALDRKILDRVYADMALIAEVCSVPEFR
                     ELLESPIILPSRKNEIIQGTLKGKIDEITASLIRLVVKNGRESYLPGIAREFVRSTKE
                     YRGITETVLTTAVRVDEETKKQIADLIAEAFKTKVELKEVVDRDIIGGFILKAEDNYI
                     DASVRNKLRKIEKELKSRTF"
BASE COUNT         3923 a         3055 c         3354 g         3961 t
ORIGIN      
        1 gcattgattg cggaaatggg aaaaaaggaa acggagtcct tcggccctgt catttctgaa
       61 ttgtcgcgaa gttacaggat cagaagcagg atacaggtta caatatcaga tcttggaggt
      121 aatcctgttc acggaatatt tcgggtatgt ggtgtgtacc ggacggccaa cacaggtttt
      181 gaccagacaa cggcttttgt aaatgaggat gagcttgtga aactctatgg cggaaaagaa
      241 attcttaccc atgaaatagc aattttgctg aataatactg atgagtccgg catggtcagg
      301 gacaaactga accggattgc cggaaacaat tcagtcagta cctggaggga actggccccc
      361 gatgcagcct tgctgaatga tttcatgatt gtgtattatt ttatttttat cggaattata
      421 atgcttgccc tggctttcgg tattataaac acgatgctga tggcggttct tgaaaggacc
      481 agggaactgg gcatgcttat ggccatagga atgaaccgcc ggagagtctt tgcaatgatc
      541 atgcttgaaa ccgtttttct gacctttacc ggagcgattg caggaatgtt atccggatgg
      601 gtgataacgg gaatactggg gaaaaccggc atttatctgt ccggatgggc cgaaggattt
      661 gaggccctgg gatttgcctc aagggtatat ccggttgtca ccattgactt tatcatgttc
      721 acctcactaa tggtaacagc cacggcaata ttagcttctg tatggccggc ccgcaaggcc
      781 ctgaaattaa ttccggctga tgctctgagt acaaaatagc aacaatgcaa aaagaaacag
      841 gccatgaaag tacttgaact gaagaatgtg aataaaatat ataattcagc agagatcaaa
      901 gtccatgcgg taaatgatgt gactttggaa tttgaagagg ctgaatttgc tgctatcgtc
      961 ggcccgtccg gatcgggcaa gacaacactt ctgaacatga tcggcggact tgacatgccg
     1021 acatccggtg aaattattat cgggggtgtt gatctttcaa ggctcaggcc gtcggaactg
     1081 atcgatttca gactgaacaa cataggattt gtgttccagt cattcaacct tattcccgtt
     1141 cttacggcaa aagagaatgt tgaattcatt atggcacttc agaaatggcc ggcagataaa
     1201 agggaggaac ggactcttga attgctgagg gccgtgggtc tggaagaaag gattaacagc
     1261 cgcccctcaa aactgtcagg cggacagcag cagagggttg cggtggccag ggcccttgca
     1321 tcccgaccga agtttgtact ggccgatgaa ccaactgcca atctcgattc aaaggctgcg
     1381 gcaatactgc ttgagataat ggaaaagctg aaccgggatg agaaaataac atttatcttt
     1441 tcaacccatg atccgagggt gatgaaaatg gcccgcaggg tgatcaccct ggaagacgga
     1501 aaggtcattt ccgatgaagg gaaggaataa aatctctttt tttcatgacg agaataaaaa
     1561 tactgttaca gctttccctg ttattcattt ccgttccggc ctttaaccag gaagatggaa
     1621 agaagattgc aataagcggt tatctgagct ccgtgcagag tgtgatgttt gatactctga
     1681 aaggcccctt cgtcaatgac tatattttac ataaccgcct caatttcaaa agctggctga
     1741 atgatcacat aacgtttgcg gcggaattcc ggaacaggct gttcacgggt gatatggcaa
     1801 aggccgggaa aacctatgcc gttctgacag gaagtgatga tggctttgtg gatatgtcct
     1861 ggaatctgat ttccggaaaa tctttctttt ttaatacaac aattgacagg ctgtgggtgg
     1921 atatcagcta tgaaaaattc caggtctgtg caggaagaca gagaataaac tgggggcagg
     1981 cactggtatg gaatcccaat gatattttca atacttattc ctttttcgat gttgactata
     2041 ttgaaagacc gggctgtgat gcggtccgcc ttcagtatta tccctcatgg tcatccgcgc
     2101 ttgaactggc tgtcagtgcc ggcagtgaga aggacatcac tgctgcaggc ctgttccgct
     2161 tcagcaggtg gggatatgat atacagttcc ttgcggggta ttcaaactcc tcggatattg
     2221 ttgccggcgc aggatggtcg gggccggccg gatcattctc attcaggggg gaaactacct
     2281 ggttccggcc tgcggaatca ttcctgaact ccaggggcac gattctggta accgccggtg
     2341 ttgacaggat attcagcgat aattcggtgg ttcagcttca gatgatgtat tgcaatgacc
     2401 cgcagggtcc ggggaccctg acaaagctgc ttgcatccgg cctgtcagcc cggaatctgg
     2461 ctttttcgaa atttaccctt ttcgggcagt gtgtgcttgc tgcaacacct ctactgaaca
     2521 ccgggctgtc atcgatgtgg tttcctgatc tgaaaggttt ctttgccgga ccttctctcg
     2581 aatggtccat ggcccagaat gtcgactttt ccttcatctg gcagcacttc aacgggaaaa
     2641 cggatgagct gcggtcaaga ataaacctga tgtttctgaa attcaggtac agtttctaga
     2701 attttcctac ttttaccgcc ataaaaaaat tatcaatggc actggttcat gaattcgaac
     2761 gaagcggcaa ctggcttttc agaagaagaa gctggctccc tctgtttctt gttgctgccg
     2821 gcatcctgat actctggggc ggaaacaggc aggcaggctc ctttaatctc cgcgatgaga
     2881 tggtttttct cgcaataagc cttactggag agataatcag gatacttaca gtgggctttg
     2941 caccacaggg aacatccgga agaaacactt ctgccggaca ggttgcatgg gagctgaatg
     3001 agaccggtgc ttattcactg gtaagacatc ccctttatgc cggaaacttt ctcatgtggc
     3061 tcggaccggt gctttttctg aggtctgcct ggtttaccgt gatatttatt ctggtatact
     3121 ggctttatta tgagaggata atgtttgccg aggaacagtt tctcagacgg aaattcgggg
     3181 agaaatatga tgaatattct tcgaaagtat atccaattat tccgtttaaa tttaatttcc
     3241 ttcccccggc acttcccttt tctctcagga atgtactgaa aagggaatat cacagctttg
     3301 tcaatatctt cctgattttt gcagcccttg atatttccag gaattacttc cttaccggaa
     3361 ggctgtttat taccgatatc tggatatggc tcacatgcgg agccggaatt atctggctga
     3421 taatccggct catccataaa ttcacggact ggcttgaggt tgaaggccgt tgaaaaccac
     3481 gcctttgcct gtgccgctta ttcccttgct cctgtgaata tcatgatata atagagcaat
     3541 gttgccagtg agcccactgc agctataaca taggtcatag ccgcccattt cagggcatcc
     3601 cgggcccttg cctggttctc atgtgaggta acccctgtac ctgaaagcca tacaagggct
     3661 ctctgacttg cattgaattc tacgggcagc gttataaagc tgaaaagggt tgtcagggcg
     3721 aacaggacaa tgccggtgaa taaaagtgcg gggaaggttt taatcatcag aatgcctgca
     3781 aggatgaccc aggtaaccca ttttgatgag aagctgacaa cgggaaccag ccttgatctt
     3841 aatgtgagcc atgcataggc atttgcatgc tgtaccgcat gcccggtttc atgagctgcc
     3901 actgctgctg ctgcaatact ccttgatgca tatacatccc ggctgagatt gatagtcttc
     3961 ttttccggat tgtaatggtc tgtaagctgt cccggcacgc ttacgacact cacgtcatat
     4021 ataccgttgt catgaagcat cttttcagct atttcctttc cggtcatacc gttttcaaca
     4081 ggtatttttg aatatttcct gaattttgat ttcagagttg aactggcgat ccagctaagg
     4141 accattactg ctatgaaaat taaccagata ttcattattg tatttgtttt taaattaact
     4201 tatttccggg ttgaattacc ttcaggactt caaaagccct gccatttgac tttcatgcca
     4261 atttgacagg gactttttaa ggatcagggt aaaaacggac agaaagcgct tttatctata
     4321 tttggaacgt ggcaaaatta cttaaggata tactgtcatt tctgctggca cttccggtgc
     4381 atgtttaccg ccatgccatt tctccctggc tcaggccttc ctgcaggcat gttccttcct
     4441 gttcggcata tatgctcgat gccctgaagc ttcacggtcc ctttaaaggc tttttgcttg
     4501 gctttgacag gattctccgg tgccggcccg ggggaacaca cggatatgat cccgtacccc
     4561 tgtttatttt ccgccggtat aaatacctgt caggaatact gagaggctgg aaaaggcaga
     4621 accgcctcag acattaagtt tcagcatcac agcaggctca gatccgcagg cctgcagtca
     4681 tattaaaaat ccagttcctt tttcagattc tgatatcccg tcctgcttac cggaagcctg
     4741 tcacccgatt tcagtacggc aatatgactg tccttgccgt agggttcgat cttgctgatt
     4801 tcttgcactc ttaccatgta tgatctgtgg atcctgataa aatcagccgg gggaagatta
     4861 tcttcataat atttcattgt ctgttgcttg agtgccctgc cctcaatgtg gtgtaccatc
     4921 acataatcgt cctgtgcctc aaggtatctt attttctcaa cgggaataat tcttattgca
     4981 ttacctgttc ttacaacgat ccttgcaacg ggtgcttccg gagcaggcaa ctgtgaaagg
     5041 atacggtttg ccgcctccct gccggcagtt ccggaacgga ttttttcagc cgccctgttg
     5101 acagcatcca aaaagcgcct tttatcaaac ggtttcagca gatagtcaac ggcattgagc
     5161 tcaaaggctc ttattgcata ctggtcatag gccgtggtga atataatttc aggcttttcc
     5221 gtcattacct caagcatttc tattcccgtc agcctgggca tctgtatgtc aaggaacaca
     5281 aggccgggtt tcatggctgt aatggctttc agtccggaaa aaccgtcact gcattccgaa
     5341 atgatatcaa tataatccag atcctggaga taatgcctta taagctccct ggccggactt
     5401 tcatcatcta tgattatcgt tctgatcttt ttcatcggtg ctgttgtttt ggaatgagac
     5461 tgtccggtaa cttccttttc ttttccggct tggcgcagga acgggtattc cggattatat
     5521 tccggatgat atgtggttca gtccggcaat gaaatacgga aaggagacct gcggcagctt
     5581 ttttctcagg tttcatgcgg aataaaaagt gttacactga atacgtcctt ctcttttgag
     5641 gttataagcc gggctttgtt accgtaaaac aatgacagcc ggttttcaac atttgccagc
     5701 cccgtaccgg ttcccttcct gaaagacggg gatgattcaa aattattgct tattgtgatc
     5761 tgcacgcagt cctctgtttt tttcacttcg gtttttatgg ttacggatcc gctgctttca
     5821 taaacgccgt gttttatggc attttcaaaa agaggctgca gtatcattac aggcagcttc
     5881 aggtcgagac actgcgtatc aatattttct tccgttgaaa gcctgtcgcc aaaacggatt
     5941 ttttctatat cggaataaag tttgagattt tccagttcat tcctgagtga tacagcctgt
     6001 tcatcctttt ttgaaagccc gtatcttatg aattcagaca gttttatcac catctctctt
     6061 gctttctccg gattggtaag ggtgagagag ctgacggaat tcaggctgtt gaacaggaaa
     6121 tgcggattga tctgtgatct gagcattttc agttctgttt cctttaaaag actttcaagt
     6181 tttgcttccc ttgctttttt ttccgacagc tttcccaggc ttataaacag ataataggtt
     6241 actacagcca ggatatagat aaaaacggat gccccggccc tgtatggcaa tgtgcttttc
     6301 cagtatatcc cgaagaataa tttatccccg gtcactgact gtaacgtcat tctggttatt
     6361 accatgctca gagaaatgaa gacagtgcct gctattatca gactaaatac aatcagaaaa
     6421 acttttgtgt tttccctgtt gatatagctg aaagggtacc atagtgacaa aatcagaaca
     6481 gaaaggatga aaaaggacag aagggtatcc ggtatgctta gcgcatcaaa gtagccgtat
     6541 atgaaataga ataacagtat ctggccggct gcaaggatga tccagaccag ccaccatatg
     6601 atcagccttg tcctgttctg aagtacagga tgtttcatgt ccggattttt cagattaata
     6661 actcttcact tcgcccccgc caaaaaccac agttcctttt attacaagct ggctttcggg
     6721 attgacgggt ctttcctgcc tcagcttccg tgaatcggtg aatcctccga ggacaggagt
     6781 cacttcaatg gtgacctgcc atgattcggg gacaattatt gttgctcctc caaaaacaca
     6841 gattatctct atctcgtttc ttcccggagc caggacggct tttgtgagat tgagttccgt
     6901 cccgccgaat acggccgtga tttttccccc tctgaagtta ttggaaacaa tctgtctgtc
     6961 cccgccgctg aaaatattga tataatcaat gtaatcatca ctcgtaagat cctttgttac
     7021 ccttcccgac catcccttcc tccttgagga aatgaaaaga atgccggcaa ttatgaatat
     7081 tgcgggccag aacaaattat acatatggaa ggtatgcctg aacacaaggg gtatcataaa
     7141 gaatccgccg acggccatta ctattatgcc tgcacttttt tccctggaat ttaccgtcat
     7201 taccaggcca ataaccacca gcagcatctg ccagctgaaa atcaccctgt cgataaaatc
     7261 agggaatatt ccggtatttc ttaacaggag aaataatccg gtaaggacaa gtatcacccc
     7321 tacggttgcc ttgttgtttt tgtcatagac atgttccctg ccctgatagc cgggatgttt
     7381 ccggtattcg tcatttgttt ccatacttgc atatttaaat atttgttttt tcagcctgtt
     7441 ttttctttct gaagaaggag gcaatgatta atgatattcc cgcaaggatt actactgagg
     7501 gccagtaaaa gactttaacc gctgccggca ttccgccgag ccggttttcg tacaaaaacc
     7561 acaccccccc ggcaatcaga agaatgcccg gcaggagact ggggttaagt atcagaagga
     7621 ccccgacaaa gatcattgct gtttcccagg taaaatattc cttaatgccg cccaggtgca
     7681 gcaggtcatt tgttgccacc aggagagcaa gtcccagaac aatgaggaaa agcccgattc
     7741 ctctccccgg atggtgctct ctctgtttct gtacaaaagt atcgcgcttt ttcatcagaa
     7801 ataatttatg atcaaaaata cttcagtgtt ttaaatagtg ttaataaaaa tcggtgaatg
     7861 ccggattttt taaggtatat cacagctgca aaggatattt tttacatttt tgttgataat
     7921 ttttttaatt ggataagctt tcatactttt gcagaccaaa ttttgttgag atgtcgggtt
     7981 tatatagcat accgataagc gggatgaagg agggctgccg gagttttgat tttgaaatag
     8041 ggaatgatct ttttgcccat tttgaggaag cagaagtaag ggaaggatcc ctgaaagcga
     8101 ttgttcaggc cgaaaagggt cctgctcaca ttgatctcac aattctcatt gacggtgcag
     8161 taaacatttc atgtgaccgg tgtctcggca tttttccgtt tccggtacat tgcattaaca
     8221 gcttgctggt taagtttgga aagacacacg atgaaagcga tccggatatt attttattat
     8281 ccgaagaaga aaatgaactg gacctgatgc agtattttta cgaatatatt attcttgcat
     8341 tgccgataca gcgtattcac cccgcggatg aaagcgggaa cagtacctgt gatccggaaa
     8401 tgataagcag gctgaacagg catttaaaaa acaacaatac cggaactgac ccgcgctggg
     8461 acgaattaag aaagattacg aacaattaaa actgattaca atggcacatc ccaaacgaaa
     8521 acaatccaag gccaggagag acaagcgcag aactcatgac aaggcaactg caccgacagt
     8581 tataagctgt tcaaactgtg gattttcagt tcagtatcac agggtatgtc ctgaatgcgg
     8641 atattaccgt ggcaagccgg ctgtagaaaa gaaaaccagc gcttaggatt tttcactaaa
     8701 cccttgatac aatgagaata ggacttgata tcatgggtgg agattttgca cccggagcag
     8761 ttatagaagg tgcaattgat acacttgttc atctttccgg cgaagacaaa ttagttctta
     8821 ttggtgatca gcaggcggtt gaatcaaaac tcagggagag tactgccgat ctttcacgtg
     8881 tcgaaattgt ccattcaaca caggttatcg gaatgggtga tatcccttcc aaggcctatt
     8941 cgcacaagcc cgattcgagt atagctgtgg gataccggct tctgaaggaa ggcaggattg
     9001 acgggttctg cagtgccggc aatacaggag caatgctggt gggcgcaagc tataccgtca
     9061 acgtcatccc gggggtttta aggccggcac ttgctgcaat tgttcccctg tttgacgacc
     9121 gcaatgcagt gattctggat gtgggtctga atcccgacac aaagcctgac gtgcttctcc
     9181 aatatgggct tctgggaagc ttctttgcaa aatatgtcct gaagactgac agtcccgttg
     9241 tgaagcttct caatattgga gaagaggaat cgaaaggaac agcagcaatc agagctgcct
     9301 atgaacttct gaaaagtcac ccgggaataa atttccaggg taattttgaa gcaaacgaaa
     9361 tgttcaggga aaataatcct gacgtacttg tttgcgatgg ttttgtcgga aatattgtcc
     9421 tgaagcagac agaggccttt taccggctgt ataaatcgag aaatctgggt gatccctatt
     9481 ttgaaaggct taattttgag aatatcggcg gaacgccggt agtgggtatt aatgcaaatg
     9541 tggttatagg gcatggaata tcgaaacgta aggccatcat gaacatgatc cttcagaccc
     9601 ttgcggtagt ccgtgtcaat ctctcgcaaa agatcaaaga atcaatctga taatgggaaa
     9661 aatacgtgca gccataactg ggattcatgc gtgggttccg gaatacaggc ttacaaattt
     9721 tgagctgtcc agaatggttg acacatccga cgaatggata atgcagcgtg tcgggataaa
     9781 ggagagaagg atcctgaaag gggaagggct cggtacctcc gaactgggtg tgaacgcagt
     9841 aaagggcctg ctggaaaaga caggaacatc acccgctgaa gtggaacttc tgatttgtgc
     9901 caccatcaca cctgacatga tgtttccggc aacggccaat ataatatccg acaaggcagg
     9961 tataaaaaat gcattcagct ttgacctgtc ggcagcctgt tcaggttttc tctttgctct
    10021 tcagaccggt gcggcataca ttgaatccgg attatgcagg aaggtggtca tagtgggagc
    10081 ggataaaatg tcatccataa ctgattatac cgaccgtacc acctgccccc tcttcggaga
    10141 cggcgccggt gcggttcttc ttgaaccgac agaagaggaa gtgggcatca tggaccatat
    10201 tctgaatgtt gacggatcgg gttgcaggta ccttcacatg aaagccgggg gatctgccag
    10261 gcctgcatcc catgaaactg ttgaagccag ggaacacttc gtctaccagg aaggacaggc
    10321 agtattcaag tttgcagtgt caagcatggc tgatgtggct gctgaaataa tggtacgcaa
    10381 caatcttaaa cctgaagata ttgcctatct ggttccccac caggctaatc tcagaatcat
    10441 tgatgccacc gggcggagaa tgggactgcc tccagaaaag gttatggtaa atattgaaaa
    10501 ttacggaaat accactgcgg caaccattcc tctctgcctc tgggattatg aaaaaaaact
    10561 gaaaaaagga gatatactca ttcttgcagc tttcggggga ggattcacct ggggctccat
    10621 ttatttaaaa tgggcctatg acccgcaata agcataatgt aatatacgta atagtatgta
    10681 aattacgcaa tagtttatat atttgcgagt tattaagttt atatcttaaa actctgataa
    10741 aatggcaaaa ttcaatgagg ctgaaatagc agcaatcaat ctgatcagca gtggaactga
    10801 tataaccggg gatataaaat caggcggaga cctgcggatt gacggcactc ttaacggcaa
    10861 tctcaatacc aagggaaagg tggttatcgg gccgacagga aaggtaaacg gagaagtagt
    10921 ctgcaaaaat tctgaggttt caggtattgt tgaagggaaa atcactgtac accagctgct
    10981 tattttaaaa gtctcttcca agatcctggg cgatatagtt actgcaaaac tatcaatcga
    11041 gccaggcgca atattttcgg gaaactgtaa aatgagtgac aatgaaaccg gaggaggagc
    11101 tggaaaagga aaagaatcag aaaaaacctc aagataaaag gctgagcagc tatgccaaat
    11161 ataccggtat ggccttccag atgggaataa taattctggc caccacctgg ggcggaatta
    11221 agatagacca gcttgccgga tttgaaaagc ctgtatttac aataatactt tcattactcg
    11281 gtgtttttgc agcaatctat actgcagtca aagactttct gaagtaaacc ttaccgtgaa
    11341 actgttttta aaaaatatta ccggcctcgt catcctgacg gtcattgtcc tttcagcggg
    11401 attgatactg gttctgaaat acggaccggg atttaatttc ggagagcttg ccctgctgac
    11461 actcaccttt gccatccttg cccttgtttc aatgacaatt attcacaaag gccggaaaaa
    11521 agatgcggat gcccaggtta ttcacctgct tgttgccgtg ggtatcaagt ttataactga
    11581 tcttgtcatc gcccttgtct ggttttttgt cctgaaaaaa accagtgtaa cattagtaat
    11641 attgtttttt gtactatatt tagccttcac tctgttttcg gtattgggtg tattgaaaac
    11701 actgaaatac aaacccttat aacagactat caagttgaaa atgcgtattg taacatttgt
    11761 atttctcatt ttactgcata ttgcatatcc tgtttccgga tcagtacaaa acggttccgg
    11821 accgcatgag gaaaaaaatc agactgaagc cggaacaaaa ttcaatgcca gtgaatttat
    11881 aatagatcac atcagtgatt cgcatgaatg gcatatcatg accctcgggg agggcgaaaa
    11941 cgagaagcat atcaccctgt accttccggt aatattatac agcaagaaaa gcggtctgca
    12001 tcttttctcc tcaaggaggc ttgcccacgg tcatgaatat cagggcttca ggctcatgca
    12061 ggagggagat ctcaagggga agattgtttc actcagtgaa aacggagaag ttgacagtga
    12121 aaacctgctg ctggattttt ccatgacaaa gacggttgtg ggtatgcttg ccgcagcaat
    12181 cataggtctt acactttttc tgctcctttc caggtcgtac agtaaaacgg gaatcagcca
    12241 tccgaagggc atacagagcc tgctggaacc agttatatgc tttgtccgtg atgatatagc
    12301 aattcccaat atcggtcatg agaaacatgc caggttcatg ccctatctcc tgtcggtatt
    12361 cttttttatt ctgataaaca acataatggg acttattccc tttccgccgc ctttcggggc
    12421 aaacgtgacc gggaatattg cagttacttt cacacttgcc tttttctcat ttgtgatcat
    12481 tcagataaac ggcagcaagt cttactggag gcatattttt gccgcacccg gcctgccggt
    12541 gtggatgctg cccctgatga tacccgtgga gatgataggt gtgatagcaa agccttttgc
    12601 acttatggtc cgtctttttg cgaatattac cgcaggacac ataattgtaa tgagtctgct
    12661 tgcgctgata tttattttca actctctctt tgttgccccg gtaacaatat tatttgtgat
    12721 tttcatggat tgcctcgagt tacttgtggc tttccttcag gcatatgttt ttacgctttt
    12781 gtccgcactg tttatcagtc tggcagtagt ggaggaacat cattaatatt ttatgtgttt
    12841 aattaattaa aatcattatg acaggaacat tagcggcgat tggagcaggc atggcagtta
    12901 tcggagccag ctatggcatc ggctggattg gaaaatccgc catggaagcg atagcccgcc
    12961 agccggaagc tgcaaacaag gtgcagacag ccatgattat tgcagcagca cttattgaag
    13021 gcgttgcatt atttgcagtt gtagttgctc ttattgctta agaaaaatga aatcctgcaa
    13081 cggttggttg caggatttct tcatttcaga aatttaacag attaatcatg atattagcca
    13141 gcaatagtct taccagccct gcagtaggaa caattttctg ggccgtgctt atcttcggca
    13201 tcttttttta catactttca aaatttgcat ggaaacccat tctcagtgcc ctgaaacaaa
    13261 gggatgacct gataaaggga tctcttgagg cagctgaaaa tgcacgcagg gaaatgattc
    13321 agcttcagag cgataatgaa gcaatcctgc aaaaggcaag ggaagagaga gagaatatcc
    13381 tgaaagaagc cagggatgtg agggataaac tgatttccga agcgaaggtc agggccacgg
    13441 aggaagcttc aaaaattgtt gaaagtgcaa aaaccggtat tgaaagggaa agaaacaagg
    13501 tcctttccga gattcatgat cagctggcta cgctgtccgt tgacattgcc tcaaagctta
    13561 taggcgaaca tctgtccggg agcggagaac aggagaagct cataagaaaa taccttgacg
    13621 atataaaaat caataagaac tgatgaacga cggtaaaata tctgtcagat atgcacgtgc
    13681 acttttcctg tcggcacttg acaggaaaat acttgacagg gtttacgccg atatggcgct
    13741 tattgcggag gtatgcagtg ttcccgaatt cagggagttg ctggaaagcc cgataatcct
    13801 gccatccagg aaaaatgaaa taatacaggg aaccctgaag gggaaaatag atgagattac
    13861 tgcttccctg atcaggcttg ttgtaaaaaa cggacgggaa tcataccttc ccggtattgc
    13921 gagggaattt gtacgctcta caaaggaata caggggtatc accgaaacgg ttctgacaac
    13981 ggcggtcagg gtggatgaag aaacaaagaa acagatagca gaccttattg cagaagcctt
    14041 caaaacaaag gtggaactga aggaagttgt cgacagggac attatcggcg gattcatact
    14101 gaaagcagaa gataattata tagatgcaag cgtcaggaat aagctgagaa aaattgaaaa
    14161 agaacttaaa agcagaacct tttagtttca ctgttttccg tcgggcattc agccgggaaa
    14221 tgaattaaga taaacaagta attaatatca gaaatatggc aagtataaaa cccgcggaag
    14281 tatcaagcat tct
//
LOCUS       DAOSPX010000044        14257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4329_length_14257_cov_4.598155, whole genome shotgun sequence.
ACCESSION   DAOSPX010000044 DAOSPX010000000
VERSION     DAOSPX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4329_length_14257_cov_4.598155"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLZ75_03795"
     CDS             <1..190
                     /locus_tag="PLZ75_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71554.1"
                     /translation="KAILFYEARIEIEKIEINTTGIPEGVILIEIDYIISATNSRFNF
                     VYPFWRQEGTELSSLLKI"
     gene            204..3962
                     /locus_tag="PLZ75_03800"
     CDS             204..3962
                     /locus_tag="PLZ75_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71555.1"
                     /translation="MADCSKNKNPLIRSGINQSQRILRELDGKFILPDGKGFKEWLVY
                     ARDLASHIKFYNTGNKEDGDWQPFFNTDVSAVLALIAIQDVNDYKDRIRLLIDSISSK
                     ELQGNAPELRRHFGLLFSAIFSLSSRMDYFTEIIPDDITLKRTISNLILTKLARVLKR
                     FIGYYKAAEAFYTPPLISDSEISDWTILGFKQVKVSEAVSLVRKLSERWIYRPLTAVN
                     YNDFYDEIPVDEKIYDHPLPVVNDPDLWKLLNHAASHNLFSSVFSEYLSVYARIVSDA
                     ESQLIKTITGHNNHEPHYALYLSFLHLFRLAQNGLNTYTRRHLDFYYKEILRLAEKKS
                     EPNHVFLIFEPARTVNEHLIKKGTAFRAGKDNEGKEIFYNTDEDIIINRSAVGRLCSF
                     YKETSTEGSNPFRGMLFASPEANSADGAGAKLTSLSGDWHPFANRIFSEGILSDINMP
                     GANVGFALASHYLFLKEGTRKITITIHGSSLRSLSDTEFLCLLTTEKKWLEKSDISVA
                     ATSTRATITININPSDPPVTAYVKKVHLDQFNNIDTPVVKFILKNISGKNQYDTLKDM
                     LITHVDLKVTVGDLNKYNQKGLKELFVSTDSGTVDPSKPFIPFGQNPKAGTGLVIGNK
                     EVFSKKGAKMTLSVKWAEMDQTIQDMDYNLTDIFFPTAQLKFLNNGIWTNWDQYSLLN
                     KEHHSAERGEVELFWGKNSIMIPDTYLPSFKATIPDECITDYRDDYVQYGISSQRGFF
                     RLELNSDFQLDKYFSDLQIFLIELANKTPQADRTVKTQPVKPYIPKIQSVFLSYEAET
                     TIRFSSPGENPSSFIHIYPFGYERIDSSNVPLNMVNLMPQFIHREDNIPVHNIAEFYI
                     GLKDVVPEQRVSILFQLLDGSTNPLQSKPAEHVFWSYLSNDGWIDFDKNEIADNTRQL
                     ISTGTINFPFTGNATSGNTILPSGYYWIKAGIKEFPGQVCRIVDVRAQAVRATFCDRN
                     NSEDFLKQPLGPETIKKFAEPDVSVKKVEQPYSSFGGRYKEDPVSFYTRASERLRHKN
                     RAITIRDFEQLILEAFPDIHKVKCLNHTKLDLQVIPAVYNELAPGHVTIITIPDLRQK
                     NAIDPLRPYTSRGRLNEIRDYLKKISNCNVSLYVENPAFEEVRIKADIVLTGDASGNN
                     TFYAGLLKQELVEFLTPWAYKTGSDIEFGGKITKSDIINFIEERSYVDFILDLKMYQG
                     TSSASQAGGDMEEIIASTARSILVSAPASSHFINILPNPKTNDNNEWK"
     gene            3940..6981
                     /locus_tag="PLZ75_03805"
     CDS             3940..6981
                     /locus_tag="PLZ75_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71556.1"
                     /translation="MTTMNGNDHITISKDNLLPQSQDYYFLRREGLKLIEQLGSRIWT
                     DYNIHDPGITILEVLCYAITDLSYRSSFSVKDLIAEEKPGMFNPETQAFFSAREILTT
                     APWTTNDYRKILIDLEEIRNAWIKCNECSCGPDIYVNCKKSELTFKKTDINKAFISEV
                     VPKGIYNVLLELDRDEQYGDLNTGKVSVSYSHLVDGNPETLYLEVRFPSGQELNRLQK
                     TDANLELFRHPESRVSNVEVKLISDKKNDSADIEESRVITALRSVMYASFEISFDEFL
                     TTPGIAKTRKVTFRDVPFRFLLLKSEIRYEMVLQDIRDILKDKTESGIAARYMLKTRL
                     ADEAVGKALKVLNSHRNITEDFCCVEEVEIEEVGVCADIDLTPEADIEEVLAEIYYRI
                     EEYFNPTIQFKTLAALLESKTVDEIFDGPKLLHGFIEDNDLEKNVLNRTLYSSDIINI
                     IMDIPGVISVRNFVLIRFDKEGKNAGPDPWKLKVSENHLPRLYIEGSKLLVFKNGLPF
                     LPDTSELLDTIKVISSRSTMPKLKDHDLDLDIPEGTYYPMKDYKPVQNSLPLVYGTGY
                     ESLPPRADDLRRAQAKQLKSYLTFFEQLLVNYLGQLANLKHLFSVDNSIKQTYFPVFL
                     GADLLKNDLYNDFTTEFYRDFDAAGLTEMTESRDLFLNRRNKFLDQLLSRFNESFSEY
                     ALLLYSYKIQKKIASENLITSKIAFLKQFPWQSANKAQGFDHTVGDYICLNKDLSGIH
                     NRISALLGIQNSLNYFDFVISKTGDGNYRSSLKLRDNDDKVLLKLADTSEDKDRDIIV
                     KQLNLNMSRILNLVIGKSNYRIKPSSGKYIIELGVPGIAISGEKFDNQTDATAAIDRI
                     VDFCKLNLSDERFIIVEHILLRPHVKGDPLLPVCVDNNCDFCGEEDPYSYKVTFVFDG
                     ESELPAKHFNFRKFAEKTIRNELPAHVMAKICWVKHDVFLAFEEAYCEWLSATSSTRS
                     LKLEKLVSIFETLKSIYPSPTLHDCIDGNDENPVLLDQTQL"
     gene            7001..11215
                     /locus_tag="PLZ75_03810"
     CDS             7001..11215
                     /locus_tag="PLZ75_03810"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOS71557.1"
                     /translation="MIPVKTDEKDFNYPNFQTDQILTASHLNHMFDFLDVQERLTRTN
                     LVGIGIVCGLKVSRSGDGKTVTITKGTGVTSHGYLIAHGIENSDDPVIYRKYRNFDAL
                     KVRTYDVFVNGNVQKYALWELIDKDHEDTNDHNLSKEFLNNKVCLLFYEILDEQAKNC
                     DPLSCDDKGVKVFINIRPLLITITDATTIINTLNAKAGASGSGEVFPGIFSLPDIRLP
                     GFDVPATSLETTSDVYNAYRKIFTKKFVEDTGEVLNKAFNTFKGYLKVAVNPFTDFSA
                     KFGFLHNGSIDGNNLLNFQYYYDFFSDLLAAYNEWRESALRLTGLCTPPGALFPRHLF
                     LGTTDNTDELSRSFYRNYFIPSPIHNICSDKYNDFLGLFSRLERLVSSLKIPVPFVSG
                     TASADANIRITPSLIGSYPLGKRAIPFYYTPDEAGRSLLKVWDPEMTSKGKERQILSY
                     NPSYNTQDDFVRNPLLYDLEPYNFFRIEGHIGKKYYTALASIQTIVKRHRLPFNIVAV
                     SVDSLPGSVDQTLYACHFNDLEAVYNELRAALLCRFCRILACIYKLPRKSAGKTKKVR
                     LIKSNLELIKGCKDVLFYSQETFGYAYEQLHPTLVKAKNSEFEILEALGKENLFKMME
                     AEKGGGTVMADNMKALSDPSLAIFFTRLILLLGKLSESLTEQLSSFDYNLMSSNVKNI
                     NTLLQEQGTDYPVLKDVFASNPCLSDLLNLCDVASFRSVYAEFIRRTADLKKMQSLKQ
                     FSASHPGLRHKGGVPKGGTFVMVYRNIEVSAAASAFEKDMLLGEFLVSDAAGGEAKAA
                     AKEKIIRTVAKTKYTAKRLSQLKTQISLMKDRGMPDQEIEEILGVSLSDLQQAAGVTD
                     DKTLWLESGTVIADFYLPYQSNSDCPPVQYVINIPPPKVTFSLEKTNYCNTDTSEYIF
                     ITNPIAGKIVTSQPDTVLDKSNGLYSFIPSKVIIPEGQNNIVLSFTYTKDDQSQTINI
                     TVYRMPEVKIIAVPNPLNPFEYNFKPEPSDSVSSADWSFGDGATASQISATHVYSSAG
                     EYKIGLDVKNVECTAKAEDITISVIEPEPVKVTIEQNEVCIDSGPVPFTITPAGGTLT
                     GEAVTEEPADSGKYIFNPSSVKLNGASKKQVTFNYTSPQGATATFSITVFDRPDGKRS
                     YDTGIRTLHLSFSGLSNTTVITADYGDGISETYTVTGLSNQTMRHSYLKGGIYTVKTA
                     LLNGPCILQNEDIVAAIEDDQPPVKVCQSFRVPVEKYFRLSDPLFNSKDFQENYSQEI
                     KNVEEFFKVLSDKLTRSGKVDLRFFDEHPVMPDWIRLLPVKPAALRTLSLQLLMIFIS
                     IITIIACNQKKDISGTMMELFKFINTKLNEMDKLNAAEKGMVQELIEYLKDEIDKLNS
                     FNEAGIKQNYLNALNKLIDVLNQKLI"
     gene            11219..12718
                     /locus_tag="PLZ75_03815"
     CDS             11219..12718
                     /locus_tag="PLZ75_03815"
                     /inference="COORDINATES: protein motif:HMM:NF039901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HOS71558.1"
                     /translation="MTAIQQHIIRRTSLKVMTGTSDGFTLQQKISNLLNGEAIEKMGR
                     LFDDLVSSEEWLILDRLDIQLGDINADELESRFADLLTEKLKGVIEDRKAQILTGAIK
                     GTGSASLMSLQEKTLDAFIVFIEKGMFPWWYEVRSHAEFENEISDAFQKFYTLTLSRP
                     GDHYLHELKRILSHEYAAARLAVQFSDKVFEQLLEFLSSQPGKAPTHGPAVTAMNRIS
                     VILAGMANRLSSGIIIPETLAHIKSLLVQKIALAKSNEEITGRWLSDVVQMLSSGDMI
                     PVRDEVLNDTELKELFSGKRIIISDDKEKRILTSSHKAEMEPHYLTQAQQSDIIREIE
                     SLEGVMVKNAGLVIVAPFLPELFNECRILEGKKITDPDMALAIMHLIVWGNIDYREYD
                     TVLSKVLCNIGSEHHLKLITRLEPDVYGKVEEMLKATVRNWGALKDTSPDGLRESFLR
                     RKGKLAFSNDSWFLHVEQNTVDILLQSLPWSIGFIRLPWMKAMLRTEWI"
     gene            12728..>14257
                     /locus_tag="PLZ75_03820"
     CDS             12728..>14257
                     /locus_tag="PLZ75_03820"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HOS71559.1"
                     /translation="MHTSSEQTRKTSTTVIRQTGIPFFARKHDMTFFQGNRQAFSSAS
                     FIQPKLAVSQPEDPQEKEADQMADRVMTMPDTQVSSAPAEKEEEIQRQEEEEPVQRTA
                     SEEIRKPDDREKDEHVSRKIIPGDGRVLVARSGRSPPGPAKSSFEHSLSSARGTGSPL
                     PGETRSFMESRFNADFSGVRIHTGELSVQMNREINSQAFTYGNDIFFNSGKYSTGTPE
                     GQSLIAHELTHTIQQGASSHISPKNTGRQKNITVAARNNNAHSTHSTHSTLHRSAANM
                     TAAVSIAKGEAGKVIANKEGPDGFRVGWPRLMEYFRTALGPEKILPEGQTGDSTDVSE
                     SQIRKFFKADNVDVVTPDNRVIKGTRDALPSWCGIFAFWSLNKAGLPMKKWTLGRMTI
                     PPEAAFSPGTTPPPGAIAYRKERSHYGLVEKSDGVSVTTINGNTAGDDHLGGEVQVQT
                     HPLSGWMAFIDPMKLIEGTVRNPDFGVEERQLSLSDLRKELFGVQRKEQSDETGKKDQ
                     EN"
BASE COUNT         4342 a         2975 c         3167 g         3773 t
ORIGIN      
        1 gaaggctatt ctgttctatg aggccaggat tgagattgaa aagatagaga tcaatacaac
       61 cggaatcccc gaaggagtga tcctgataga aatcgattat atcatcagcg cgaccaacag
      121 ccgttttaac ttcgtatatc cgttctggcg gcaggaaggg acagaattat catcattatt
      181 aaaaatctga gaattgtatc taaatggcag attgttcaaa aaataaaaat cccctgattc
      241 gcagcggtat aaaccagagt cagcgtatcc tccgggaact tgacgggaaa ttcattctgc
      301 ctgacgggaa aggcttcaaa gaatggcttg tttatgcaag agacctggca tctcatataa
      361 aattctataa cacaggcaat aaagaggatg gcgactggca accatttttt aacaccgacg
      421 tttcggctgt actcgctctt attgccatcc aggatgtcaa tgactataag gacaggatcc
      481 gtttattgat tgattcaatc tcatctaagg aacttcaggg aaacgcacct gaactgcgaa
      541 ggcatttcgg gcttcttttc agcgcaatat tttcgcttag cagcagaatg gattatttca
      601 cagagataat tcccgatgac ataactctta agcgcaccat atcaaacctg atccttacaa
      661 aactggcccg ggtattaaaa cggtttatag gctattacaa agctgctgag gctttctata
      721 caccgcctct catttctgac agtgaaatca gtgactggac catactcgga tttaaacagg
      781 tcaaagtatc agaggccgtg tcgcttgtca gaaaattatc tgaaagatgg atctacaggc
      841 cattaactgc tgtaaactat aacgacttct acgatgagat ccctgttgat gaaaagatct
      901 atgaccatcc tcttcctgta gtaaatgatc cggacctctg gaaattgctc aaccatgctg
      961 ccagccataa tcttttttcc tccgtatttt cagaatatct ctctgtttat gccagaattg
     1021 tgagtgatgc agagtcacag ctgataaaga ccataaccgg acataataat catgaacctc
     1081 attatgcgct ttacctttca ttcctgcatc ttttcaggct ggctcaaaac ggactgaaca
     1141 cttataccag gcgtcatctt gatttttatt ataaagagat tctgaggctg gctgagaaaa
     1201 aaagtgagcc aaaccatgtg tttctgatct ttgaacctgc ccgaaccgta aatgaacatc
     1261 tgatcaaaaa gggaaccgca ttcagggcag gaaaagacaa tgaggggaaa gagatttttt
     1321 acaataccga tgaggatata atcataaaca gatcggcagt cggcaggttg tgctctttct
     1381 ataaggaaac atcaactgaa ggcagcaatc cgttcagggg aatgttattc gcttcaccgg
     1441 aagcaaactc tgcagacggt gcgggtgcca aacttaccag cttatcgggt gactggcacc
     1501 cgtttgcaaa caggatattt tcggaaggaa tattatcgga catcaatatg cccggagcta
     1561 atgtgggatt cgccctggca tcacattacc tcttcctaaa ggaaggtaca aggaagatca
     1621 caattaccat tcatggcagt tcactccggt ccctgtcaga tacagagttc ctttgtttac
     1681 tcaccacgga gaagaaatgg cttgaaaaga gcgatatatc tgtagcagca acatcaaccc
     1741 gagccaccat taccataaat atcaatcctt ccgaccctcc tgttacagca tatgttaaaa
     1801 aggtgcatct cgatcagttt aataatatcg atacaccggt tgttaagttc attttaaaga
     1861 acattagcgg gaagaaccag tatgacacac tgaaggatat gctcataact catgtcgacc
     1921 tgaaagtaac tgttggtgat ctgaataaat acaaccagaa gggccttaaa gaattgtttg
     1981 tatccactga ttcgggaact gtcgatcctt caaaaccgtt cattcctttt ggtcagaatc
     2041 caaaggccgg taccgggctg gtgattggta ataaggaggt tttcagcaag aaaggggcta
     2101 agatgaccct gtcggtaaaa tgggctgaga tggatcagac aatacaggat atggattata
     2161 atctgaccga cattttcttt ccgactgcac agctgaaatt tctgaacaac ggaatatgga
     2221 cgaactggga ccagtacagt cttctgaata aagagcatca ctcggctgaa cggggtgaag
     2281 tagaactatt ctggggtaag aattcgatta tgatccccga tacctatctg ccttcattta
     2341 aagccacaat acctgatgaa tgcataaccg attacaggga tgattacgtt cagtatggca
     2401 tttcaagtca gcggggattt ttcagacttg aactcaactc cgattttcag ttagataaat
     2461 atttttctga tctgcagatc ttcctgatag aacttgccaa taagacacct caggccgaca
     2521 ggacggtgaa aacccagccg gtaaaacctt acatacctaa gatacaatcg gttttcttaa
     2581 gttatgaggc cgaaaccacg ataaggtttt catcacccgg ggaaaatcct tccagtttca
     2641 tacacatcta tccatttggc tatgagagga ttgacagcag caatgttccc cttaacatgg
     2701 tgaaccttat gccgcaattc attcaccgcg aagacaatat accggttcac aacatagctg
     2761 aattttatat aggactgaaa gatgttgttc cggagcaaag ggtttccata ctttttcagt
     2821 tacttgatgg ttcgacaaat ccgcttcaga gcaaaccggc agagcatgta ttctggagtt
     2881 atctgagcaa tgacgggtgg atagactttg ataaaaatga aattgccgac aacacacgtc
     2941 agcttatctc gacaggcaca ataaactttc cgtttaccgg caacgctacc agcgggaaca
     3001 ccatattgcc gtcaggttat tactggataa aggccggcat caaagaattc cccgggcagg
     3061 tttgcaggat tgttgatgtg agggcccagg ctgtaagggc aaccttttgc gacaggaata
     3121 attcagagga ttttttaaag cagcctcttg gtcccgaaac cataaaaaag tttgccgagc
     3181 ctgatgtgtc agttaagaaa gttgaacagc catattcctc attcggagga agatataagg
     3241 aagatccggt aagcttttat acccgtgcga gtgaacggtt acggcataag aaccgtgcga
     3301 taacgatccg cgattttgaa cagctgatac tggaggcatt ccccgatatt cataaggtta
     3361 aatgtctgaa tcatacaaaa ctagatctgc aggtgatacc tgccgtttat aatgaactgg
     3421 ctcccgggca tgttaccatt atcaccattc ccgatctccg ccagaagaat gccattgatc
     3481 ctctcaggcc ctatacaagc cgtggccgac tgaatgagat aagagattat ctcaaaaaga
     3541 tcagcaactg caatgtcagt ctgtatgtgg agaatccggc ctttgaggag gtaaggatca
     3601 aagccgatat tgtcctcacc ggcgatgcat cgggcaataa cactttctat gccggacttc
     3661 tgaagcagga acttgtggaa ttccttactc cctgggcgta taagacaggt tctgacatag
     3721 agttcggcgg taagatcaca aagagcgata tcataaattt tatcgaggag agaagttatg
     3781 tcgattttat tctcgatctt aaaatgtatc agggcacttc atccgcctca caggcgggtg
     3841 gcgatatgga agaaataatt gcctccactg caaggtccat tcttgtcagc gctccggcaa
     3901 gcagccattt cattaacatt ctgccaaacc caaaaacaaa tgacaacaat gaatggaaat
     3961 gaccatatta ccatttcaaa ggacaatctt ctgcctcaat cccaggatta ttatttttta
     4021 agaagggaag ggctgaagct cattgaacaa ctgggaagca ggatatggac cgattacaat
     4081 attcatgatc ccggtattac catcctggaa gtattatgct atgcaataac tgatctttca
     4141 taccggtcgt cgttcagcgt aaaagacctg attgcagagg aaaagcccgg aatgttcaat
     4201 ccggagacac aggcattttt ttctgcacgt gaaatactta ccaccgcacc gtggactaca
     4261 aatgattacc gcaagatact tatcgacctg gaggagatac ggaatgcctg gataaagtgt
     4321 aatgaatgca gttgtggtcc cgatatttat gtgaactgta aaaagagcga actgactttt
     4381 aagaaaactg atattaacaa agcctttata agtgaggtgg ttccgaaagg tatatataat
     4441 gttcttcttg agcttgacag ggatgaacaa tatggtgacc tgaataccgg taaggtgtca
     4501 gtttcataca gccacctcgt agatggtaat cctgaaacac tgtaccttga ggtacgattc
     4561 ccatcgggac aggaacttaa caggcttcag aaaacagatg ccaaccttga acttttcaga
     4621 catcctgaaa gcagggtaag taatgttgaa gtaaaactta tatcagataa aaagaatgat
     4681 tctgccgata tcgaggaaag cagagtgata acggctttaa gaagtgtcat gtatgcaagc
     4741 tttgaaatat cgtttgatga gttcctgacc actcccggca ttgccaaaac acggaaagtg
     4801 acattcaggg atgtgccttt caggttttta ctgcttaaaa gcgagatcag gtatgagatg
     4861 gttctacagg atatcaggga tatcctgaag gataaaaccg agtcgggtat cgcagccagg
     4921 tatatgctaa agacccggct ggctgacgaa gctgtcggga aagctttaaa agtattgaac
     4981 agtcacagga acatcacgga agatttctgc tgtgttgagg aggttgaaat tgaagaggtg
     5041 ggggtctgtg cagatattga tctgacacct gaagcagata tcgaagaggt gctggccgaa
     5101 atatattacc ggattgaaga atatttcaat ccgacaatac aatttaaaac tctggccgca
     5161 ttgcttgaat caaaaactgt tgatgagata tttgacgggc ctaagctttt gcatggattt
     5221 attgaagata atgatctgga aaagaacgta ttaaacagaa ctctctattc ttcggatatt
     5281 atcaatatca taatggatat ccccggcgtg atcagcgtgc ggaattttgt acttataaga
     5341 ttcgacaagg aggggaagaa tgccggcccc gatccctgga agcttaaagt ttcagaaaat
     5401 catttaccca ggttatatat tgagggttca aagcttcttg ttttcaagaa cggactgcct
     5461 tttcttcccg atacatccga actgctggac actattaagg ttataagcag ccgaagcacc
     5521 atgccaaagc tgaaagatca tgaccttgac cttgatatcc ctgaaggtac atactacccg
     5581 atgaaagatt ataaacctgt acagaattcc cttccactcg tctacggtac cggttacgaa
     5641 agtctgcccc cccgggcaga tgatctgaga agagcccagg caaaacaact gaaatcatat
     5701 cttacatttt tcgaacaact cctggtaaac tatcttggtc agctggctaa cctgaaacat
     5761 ctcttttctg ttgacaacag tataaaacag acatacttcc ctgtctttct gggtgctgat
     5821 ctcctgaaga acgatcttta taatgatttt accactgaat tctacagaga ttttgatgcg
     5881 gccggtctta ctgagatgac tgagagcagg gatctcttcc tgaaccgcag aaataaattt
     5941 cttgatcagc ttttgtcccg tttcaacgaa agcttttctg aatacgctct gcttctgtat
     6001 tcgtacaaga ttcaaaaaaa gatcgcatcg gaaaatctca ttacttcaaa aatcgcattc
     6061 ctgaaacagt tcccgtggca gagtgcaaac aaagcacagg gctttgatca taccgtggga
     6121 gattatattt gtctcaataa ggacctgagc ggtattcata accgcataag cgctctgctg
     6181 ggaatacaga actcactgaa ctattttgac tttgtgatct caaaaacagg agatggtaat
     6241 tacagatcgt cacttaagct ccgcgacaat gatgataaag tattgcttaa actggcagac
     6301 acttctgaag ataaagaccg tgacattatt gtaaagcagc ttaacctgaa tatgtcacgt
     6361 attttaaatc tggttatcgg caagtcaaat tacaggatta aaccttcctc ggggaaatac
     6421 atcattgagc tgggagtacc tggtattgca atatccggag aaaaatttga caatcaaact
     6481 gatgcaactg ccgcaattga caggattgtt gatttctgca agctcaatct gagcgatgaa
     6541 agatttatta ttgttgagca tatactttta cgtccacatg taaaaggtga tccgctgtta
     6601 ccggtatgtg ttgacaataa ttgtgatttc tgcggtgaag aagatcctta ttcatataaa
     6661 gtgacatttg tttttgacgg tgaatctgaa cttcctgcaa aacatttcaa cttcagaaag
     6721 ttcgctgaaa agactatccg gaacgaactt cccgcacatg ttatggccaa gatctgctgg
     6781 gttaaacatg atgtcttcct ggcttttgaa gaagcttatt gtgaatggtt aagtgcaaca
     6841 tcatcaacca gatcccttaa gctcgaaaag ctggtcagta tattcgaaac acttaaaagc
     6901 atatatcctt ctcctactct gcacgattgc atcgacggta atgatgagaa cccggtgctt
     6961 cttgaccaga ctcaattgta aatgaaaaaa atcaactgcc atgataccag taaaaactga
     7021 cgaaaaagat tttaattatc cgaattttca gactgatcag atcctgactg ccagtcatct
     7081 gaaccacatg ttcgattttc tggatgtaca ggaaaggctg acacggacca acctggtggg
     7141 aatagggatc gtgtgcgggc tgaaagtttc cagatcggga gacggtaaaa ccgtaaccat
     7201 taccaaaggt acgggagtta cgtcacatgg gtacctgatt gctcatggca ttgagaacag
     7261 cgatgacccg gtaatttata ggaaatacag gaattttgat gctctgaaag tcaggacata
     7321 tgatgtattt gtcaatggta atgttcaaaa atatgcatta tgggaactta tagataaaga
     7381 ccatgaagat acgaacgacc ataacctcag caaagaattc cttaacaata aggtatgtct
     7441 ccttttttat gaaatacttg atgagcaggc aaagaactgc gatcctttat cgtgtgatga
     7501 taaaggagta aaggttttta tcaatatccg gcctcttctc attacaataa ccgatgcaac
     7561 aacaataata aataccctga acgcaaaagc aggtgcatca ggctcagggg aagtattccc
     7621 cggtatattt agtttaccgg atatcaggct gccagggttt gacgttccgg caacaagtct
     7681 tgagactacc agtgacgttt ataatgcata caggaaaatt tttacaaaaa agttcgttga
     7741 agacaccggt gaagttctta ataaagcttt taatactttc aaaggatatc ttaaagtagc
     7801 agttaatccc tttactgatt tcagtgcaaa attcggattc cttcataacg ggagcattga
     7861 tggtaacaac ctgttgaatt tccagtacta ctatgatttt ttctctgacc ttcttgctgc
     7921 ctataatgaa tggagggagt cggcgctgag acttacaggt ttgtgcacac cacccggggc
     7981 actgttcccc cggcatctgt tcctcggtac caccgacaat acagatgagc tttccaggag
     8041 tttttacagg aattatttta ttccgtcacc cattcataat atctgttcag ataaatataa
     8101 tgatttcctg ggattgttca gccggcttga aagactggtt tcaagcctta aaatacctgt
     8161 gccttttgtt tcaggtacgg cttcagccga tgccaacatc aggataaccc cttcactgat
     8221 aggatcatat cccttgggga aacgagctat tccattctat tatacaccag atgaagccgg
     8281 caggagcctt ctgaaagtgt gggatcccga aatgacatcc aaaggaaagg aacggcagat
     8341 attaagctat aatccgtcgt ataacaccca ggatgatttt gtcagaaacc cccttcttta
     8401 tgatctcgaa ccctataatt ttttcaggat tgagggtcat atcgggaaaa agtattatac
     8461 agcccttgct tccattcaga ccattgtaaa gagacaccgg ctgccgttca atattgttgc
     8521 agtttcggtc gattcactgc caggcagtgt cgatcagact ctgtatgcct gccattttaa
     8581 cgatctggag gctgtttaca acgaactgag ggctgctttg ctgtgccgct tttgcagaat
     8641 acttgcatgt atctataagc ttccaagaaa gtcagcggga aaaacaaaaa aagtcaggct
     8701 gattaagagc aacctggaac ttataaaggg ttgcaaggat gtacttttct acagccagga
     8761 aacattcggt tatgcttatg aacagcttca tcccacactg gtaaaggcaa aaaacagtga
     8821 atttgaaata ctcgaagctc tcggtaagga gaaccttttt aagatgatgg aagctgaaaa
     8881 aggcggcggt acggtgatgg ctgataacat gaaggctctg agtgacccgt ccctggcaat
     8941 tttctttacc aggcttatac tacttcttgg aaagttatca gaatcactta ctgaacagct
     9001 tagcagcttt gattataacc tgatgagctc aaatgtaaaa aacatcaata cccttcttca
     9061 ggaacaaggc acagactatc ctgttcttaa agatgtcttt gcctcaaacc cctgtctcag
     9121 tgatctgtta aatctctgtg atgttgcatc attccgttcg gtttatgccg aatttatcag
     9181 gcggacagct gatctgaaaa aaatgcagtc gctcaagcag ttcagcgcct cacatcccgg
     9241 cctgcgccat aagggtggcg tgccgaaagg aggtaccttc gtgatggttt acagaaatat
     9301 agaggtttct gccgctgcct ctgcatttga aaaagatatg cttctgggtg aatttttagt
     9361 atcggatgct gccggtggcg aagcaaaagc tgccgctaaa gaaaaaataa tcagaacagt
     9421 agcaaaaaca aaatatacag ctaaaagact gtcccagttg aaaacacaga tttccctgat
     9481 gaaagacaga ggaatgcctg atcaggagat tgaggagata cttggtgtca gcttatctga
     9541 cttgcagcag gcggcagggg ttactgatga caaaacattg tggctcgaga gcggaactgt
     9601 catagctgat ttttaccttc cgtatcagag caattccgat tgtccgcctg ttcagtatgt
     9661 tattaacatt ccaccgccga aggttacttt ctcccttgaa aagacaaact attgtaatac
     9721 tgatacttcg gaatatattt ttataactaa tcccatcgct ggtaaaattg tcacaagcca
     9781 gcctgatact gttcttgaca agagtaacgg actatattct ttcataccat caaaagtgat
     9841 cattcctgag ggtcagaata atattgtcct gtctttcaca tatacaaaag acgatcagtc
     9901 tcagactata aatataactg tttacaggat gccggaagtg aagataattg ccgtaccaaa
     9961 tcctttaaat ccgtttgagt ataacttcaa acctgaaccg tctgattctg tttcttctgc
    10021 tgactggagt tttggagatg gtgcaacagc ctcacagata agcgcaactc atgtttacag
    10081 cagtgcaggg gaatataaga tcggtcttga tgttaaaaat gttgaatgta cggcaaaagc
    10141 ggaggatata actatatctg tcattgaacc tgaaccggtt aaagtgacta ttgagcaaaa
    10201 tgaagtatgc atcgattccg ggccggttcc tttcacgatt actcctgctg gaggcacgct
    10261 tacaggtgaa gccgttacag aagaacctgc ggacagcggc aaatatatat tcaatccatc
    10321 ttctgttaaa ctgaacggag cctcaaaaaa acaggtaaca ttcaactaca cttcaccaca
    10381 gggagccaca gcaaccttca gtatcaccgt ttttgacaga ccagacggca agagaagcta
    10441 tgatacaggt atacgcacac ttcatctgtc attcagcggt ctgtccaata ccaccgttat
    10501 aactgccgat tatggcgacg ggatctcaga aacatatacc gtaaccggtc ttagtaacca
    10561 gaccatgaga cattcttatt taaaaggagg aatatacact gtaaaaacgg ctctgttgaa
    10621 tggtccgtgt atcttgcaaa atgaagatat tgttgcagcc atagaagacg atcagcctcc
    10681 cgttaaagtt tgccagtcat tcagagtccc cgtcgaaaaa tatttcagac taagtgatcc
    10741 gttattcaat tcgaaagatt tccaagagaa ttattctcag gagattaaaa atgttgagga
    10801 attttttaaa gttctttcag ataaacttac ccgttcaggg aaagtcgatc tgaggttttt
    10861 cgacgagcac ccggtaatgc cggactggat cagactttta cctgtaaagc ctgccgcgtt
    10921 acgcacactc tccttacagt tattgatgat ctttatcagc atcatcacta tcattgcctg
    10981 caaccagaaa aaagatataa gcggcaccat gatggagctg tttaaattca tcaatactaa
    11041 attaaatgaa atggacaagc tgaatgccgc tgaaaaggga atggttcagg aactcatcga
    11101 ataccttaaa gacgagattg ataagctgaa tagttttaac gaagccggca taaaacaaaa
    11161 ttatctgaat gctttgaata aactgataga tgttcttaac cagaagttga tctgataatt
    11221 gactgcgata caacaacata ttatacggag aacctctttg aaggtaatga caggaacatc
    11281 ggacggattt accctgcaac aaaaaatatc caatctgttg aatggtgaag cgattgaaaa
    11341 aatgggcaga ttgttcgatg atctggtaag cagtgaggaa tggctgatat tggaccgcct
    11401 tgatattcag cttggtgata taaatgccga tgaactggaa tcaaggtttg ccgatctgct
    11461 gactgaaaag ctgaagggag tgattgagga caggaaggct cagattttga ccggtgcaat
    11521 caaaggcaca ggttccgcat cgcttatgtc gttgcaggaa aaaacattgg atgctttcat
    11581 tgtttttatt gaaaagggta tgtttccctg gtggtatgaa gtaagatcac atgctgaatt
    11641 tgaaaatgaa atttcagatg catttcagaa attttatact cttactctaa gccggccggg
    11701 agatcattat ctgcatgagc tgaaaaggat actgtcacat gaatatgccg ccgccaggct
    11761 tgcggtacag ttcagtgata aggtttttga gcagttgctg gaatttttat cttctcagcc
    11821 gggcaaagct ccgacccatg gtccggcggt tactgcaatg aacagaattt ctgtaattct
    11881 ggcgggtatg gccaacagac tttcatccgg aattattatc ccagaaacac ttgcacatat
    11941 aaaatcattg ctggttcaga agatcgccct ggcaaagtca aacgaagaaa ttacaggccg
    12001 gtggttatca gatgttgttc agatgctttc atcaggtgat atgattccag tcagggatga
    12061 agttctcaat gacacggaac tgaaggaatt attttccggg aagcgcataa ttatttctga
    12121 tgataaggaa aaaaggattt taacatcgtc gcataaggct gaaatggagc ctcattattt
    12181 aacacaggca caacaatctg atattataag ggagattgaa tcactggaag gggtgatggt
    12241 aaaaaatgcc ggactggtca ttgtggcacc ttttcttcct gaattattca atgaatgccg
    12301 gatacttgaa gggaaaaaaa taaccgatcc tgacatggcc cttgcaataa tgcatcttat
    12361 tgtatggggt aatattgatt acagggaata tgataccgtt ctaagcaagg tgctgtgtaa
    12421 tattggaagt gaacatcatt taaagctgat aaccagactg gaacctgatg tttacggcaa
    12481 ggttgaggag atgctgaagg cgactgtcag gaactgggga gcgctgaagg atacttcgcc
    12541 cgatggactc cgggaaagtt ttctccggag aaaaggtaag ctggctttta gtaatgacag
    12601 ctggttcctg catgttgagc agaacacggt tgacatactt ctgcaaagcc tgccctggag
    12661 cataggattc atcagacttc cctggatgaa agcaatgtta agaactgaat ggatttaaaa
    12721 ctgaagtatg catacttctt ccgagcagac aagaaagact tctaccaccg tgatcagaca
    12781 gacgggaata cccttttttg cccggaagca cgatatgaca tttttccagg gaaaccgtca
    12841 ggcattttcc tctgcatcgt tcattcagcc caaattagca gtaagtcagc ctgaagatcc
    12901 tcaggaaaag gaagccgacc agatggctga cagagtgatg accatgcccg atacccaggt
    12961 ttcgtcagcg ccggcagaaa aggaagagga aattcagcgc caggaagaag aagaaccggt
    13021 tcagagaacc gcttcagaag agatcaggaa accggacgac agggagaaag acgaacatgt
    13081 gagccggaag ataatcccgg gtgatggccg ggtactggtc gcacgctcag ggaggagccc
    13141 gcctggtccg gcaaaaagtt catttgaaca ttccctttca tctgccagag ggaccggatc
    13201 gccgttacct ggtgaaaccc gttcctttat ggaaagccgt tttaatgccg atttctcagg
    13261 tgtgcgtatt cataccgggg aattatccgt tcagatgaac agagagatca attctcaggc
    13321 atttacctac ggcaatgata tttttttcaa cagcggcaaa tactctacag gtaccccgga
    13381 ggggcaaagt cttattgccc atgaactgac tcatacaata cagcagggag ccagcagtca
    13441 tatttccccc aaaaacacgg gaaggcaaaa aaacattact gttgccgcaa gaaataataa
    13501 cgcccattct acccattcta cccattctac ccttcacagg agtgctgcaa acatgacggc
    13561 agcagtcagt atcgccaaag gggaagccgg gaaagttata gccaataagg aaggccccga
    13621 cggtttccgt gtcgggtggc cccgtctgat ggaatatttc aggactgccc ttggtccgga
    13681 aaagatacta cctgaagggc agacaggaga ttccacagac gtatctgaat ctcagatcag
    13741 aaagtttttt aaagcagata atgtcgatgt cgtaacaccg gataacaggg tgataaaagg
    13801 aacccgtgat gcattaccca gctggtgcgg aatttttgct ttctggtcac tgaacaaagc
    13861 agggctgcca atgaaaaaat ggaccctcgg cagaatgact attcctccgg aggcagcctt
    13921 ttctccagga acgactcctc ctccgggtgc aattgcctac cgcaaagagc gctcacatta
    13981 cggactggtc gaaaaatcag acggagtaag cgttacaacc attaacggca ataccgccgg
    14041 cgatgatcat ctgggaggtg aggtccaggt gcagacccat cctctttcag gctggatggc
    14101 ttttattgat cccatgaagc ttattgaagg aactgtccgc aatcctgact tcggggttga
    14161 agaacggcaa ttatcgttaa gtgatctcag gaaagagctt tttggagttc agaggaagga
    14221 gcagtcagat gagaccggta aaaaggatca ggaaaat
//
LOCUS       DAOSPX010000045        14203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4353_length_14203_cov_5.636415, whole genome shotgun sequence.
ACCESSION   DAOSPX010000045 DAOSPX010000000
VERSION     DAOSPX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14203
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4353_length_14203_cov_5.636415"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1806)
                     /locus_tag="PLZ75_03825"
     CDS             complement(73..1806)
                     /locus_tag="PLZ75_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOS71560.1"
                     /translation="MKKVILMAFHVLLMAQGRSVPPARPECCLSSPGVMPGDVVITEI
                     MADPVPSLGLPGEEYLEILNRSRHTIDLTNWRLSSATQHSYFPEISIEPGEYLILCAV
                     SDTGLFTGYGRVAGLKPFPALTNRGRLVLLYDDRGNLINGVDYSSNWYGNSLKSKGGW
                     SLEIIDPGFPFFGEGNWEASSDRKGGTPGKINSAHRSNPDNSFRGITNVFPADNMKII
                     IEFSEPLIGFGNQTGSITVGESPVTGWELSDPLYRSFTLIPGLPLERGRIYTLGLPAE
                     ISDFAGNCPAVYDFRFGIPEPAGAKDLVFNELLFNPLPDNPDYIEFVNISGKILDASE
                     FMVSSVKEGSGAISDPLSISTGPRCIIPGTCYTITTDRLKVISGFPLSAGENIHEVPA
                     LPSMPDARGHLLLMNRQLERIDEVIYSEKMHYSLLSGKEGISLEKIRPELPSDERTSW
                     HSASGSSGWGTPGNRNSVFTADDNLTETVVLSSRKISPDNDGHEDVLVIGINSDDPGS
                     IVSVLIFDETGSCVRKLAENHLAGFRASLVWDGCGNDGRPVRRGIYILAVEIYNAGGR
                     VSKWKKVCAVI"
     gene            1894..2883
                     /locus_tag="PLZ75_03830"
     CDS             1894..2883
                     /locus_tag="PLZ75_03830"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOS71561.1"
                     /translation="MKVAVVGATGMVGRTMMNILEERQFPLTEIIPAASERSAGKEIT
                     FRGRTVKIVSVREAVDRKPEFAIFSAGASVSKEWAPVFAGNGTVVIDNSSAWRMDKNI
                     PLVVPEINSHVIRRGDRIIANPNCSTTQMVMALAPLHRKYRIRRIVVATYQSVTGTGV
                     EAVRQMENERKGISGEMVYPHPIDLNCFPHGGAFKEDGYTTEEQKLVDETRKILEDQS
                     IMVTATAVRIPVVGGHSEAVNVEFEKEFDIDEVKALISAFPGNIIHDDPFRNEYPMPL
                     MAHNRDEVFVGRIRRDFSREKCLNLWVVSDNIRKGAATNAIQIAEYMLENKLY"
     gene            complement(2933..3742)
                     /locus_tag="PLZ75_03835"
     CDS             complement(2933..3742)
                     /locus_tag="PLZ75_03835"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOS71562.1"
                     /translation="MLEKIYETTDFLRERGFVNPDAGIILGTGLGGLTAKMENCIEMD
                     YRDIPNFPVSTVDGHDGKLIYGDFGNRKVVAMKGRFHYYEGHGPEKVSFPVRVLKYLG
                     IKCLFLSNASGGLNPAFEIGDIMIITDHINLLPNPLIGPNDDRIGVRFPDMGEAYDGY
                     LIRKAAEIAGEHGIRVRKGVYLSTTGPTFETPAEYRFFRTAGADAVGMSTTTEVIVAR
                     HMNLPCFAVSIITDLGVEGKIEYNSHEAVLKEAAKAEDKMTIIMTELISSL"
     gene            complement(3714..4790)
                     /gene="lpxK"
                     /locus_tag="PLZ75_03840"
     CDS             complement(3714..4790)
                     /gene="lpxK"
                     /locus_tag="PLZ75_03840"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOS71563.1"
                     /translation="MGNRRSIFLYPFSIIWGIITGFRNFLYDKKILKSRKFSLPVICI
                     GNITVGGTGKTPHALYLAGLLGSRYNVAVLSRGYRRKTKGFLLAGPSSGTADIGDEPL
                     LISRKFPDITVAVDRDRCAGITRILEERPQTGVIVLDDGFQHRRVTPGLSILLSDYDR
                     PVYRDHLLPYGNLRESRGNLKRADIVIITKTPEDISPEEQKAIRESLDLAPGQQLFFS
                     SLVYGDPVPVFKGNTLPGPSSGDPDNGILLLTGIGNPAPLYNHLRKSYSRVIHLRFSD
                     HHYFRPGDIRKIQKILTGQKEELKYVITTEKDAVRLRETGSVAEPLLPVLYYIPVEID
                     FPDHRKEEFNKLITDYVGKNLRNN"
     gene            complement(4794..6566)
                     /gene="sppA"
                     /locus_tag="PLZ75_03845"
     CDS             complement(4794..6566)
                     /gene="sppA"
                     /locus_tag="PLZ75_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOS71564.1"
                     /translation="MKNFLVYVLATITGIIIASILFFLVMTASLSIIIASGNKSVSLA
                     DNSVLVLKTGIPVPDRTDPNPFAGLDVLNMTITPTPGLNDILRNIEKAANDRKIKGIL
                     IENGILASGWATTEEIRNALLKFRETSNKFVIAYSEYLLPQAGYYLSSAADKVYINPA
                     SVLDFKGLSGEIMFYRKALEKLGVEVQVIRHGKFKGAVEPYILDRLSSENREQIKDYV
                     GSIWNHVAAKIAESRGMTTGRVNSLADKLTGYNADDAFESGLVDGLIYRDQLIDTLKI
                     LSGLGTDDKIAMVPMHKYTKVPDPKKVLTTKTRIAVIYASGNITDGKGNESNIGGDRF
                     AEIIRKERKDSTVKAIVLRVNSRGGSAIASDIMWRELDLAAKTKPLVISMGNYAASGG
                     YYISAPGTKIYAGPTTISGSIGVFGLFPNTGKLMEQKLGINTETVNTNENSDFPSVFR
                     PMTSYEKQVMQAAVEKTYSDFIGKVGEGRKMSSESVDNIAQGRVWSGSSAMRLGLVDS
                     TGGLTDAIKGAAALAGIEKYSIRELPVAEDPYMKLISQLGSEIRSAFLKKELGESVRF
                     YRELKEIKELSGIQARLPYLIEIN"
     gene            6669..7166
                     /gene="folK"
                     /locus_tag="PLZ75_03850"
     CDS             6669..7166
                     /gene="folK"
                     /locus_tag="PLZ75_03850"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOS71565.1"
                     /translation="METVFLGLGTNLGEREENLRKAVLLIEEHIGKPVAESPVYETEP
                     WGFTSEISFLNMAVKVETDLSPSGLLGRTLMIEAMMGRLREGKGYKSRIIDIDILLFG
                     SRVIETRALQIPHPRLHERRFVLVPLSGIASDTVHPVLGKSIGELLAECRDGNKVAEY
                     RKNNL"
     gene            complement(7177..7860)
                     /gene="radC"
                     /locus_tag="PLZ75_03855"
     CDS             complement(7177..7860)
                     /gene="radC"
                     /locus_tag="PLZ75_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOS71566.1"
                     /translation="MSLKITDWAAEDRPREKLIHKGTSSLSNAELLGILISSGTRDKS
                     AVDLGRELLSMAKNNLNNLGKLTISDFTKLNGIGPARAVTIAAALELGRRRKLAEAES
                     FVLIKSSKDVADIFQPILSDLAHEEFWILYLNRSNKVISRMKLSQGGISGTVIDVRLI
                     LKKAVECLASGIILCHNHPSGNLNPSEADTKITKKIREAGSYMDVQLLDHLIISEKEY
                     FSFADNGLL"
     gene            complement(7957..8211)
                     /gene="rpsT"
                     /locus_tag="PLZ75_03860"
     CDS             complement(7957..8211)
                     /gene="rpsT"
                     /locus_tag="PLZ75_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOS71567.1"
                     /translation="MAYHKSAKKRIRQTETRNARNRYYAKTMRNALKTIRTSTDKTEA
                     SELLPKMNSMLDKLAKKNVIHHSKAANLKSSVAKHVNSLK"
     gene            complement(8286..9209)
                     /locus_tag="PLZ75_03865"
     CDS             complement(8286..9209)
                     /locus_tag="PLZ75_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOS71568.1"
                     /translation="MRKISYAALPVLIAMLAASCAPVYRCGEPRPAKTPLTWSKNLRD
                     VVRERDIVCAELELKEAENAGLTSSLAEMTKMHNEVRNQYNDQLAANRELQEKYNTLI
                     DNSLSRTEQLNQALMAKSAELDRKERLLSEREETLREMQKIIARQDSITKRLNSILHN
                     ALLGFNPDELSLEIKNGKVYVSMSDKLLFRSGSAAVESKGREALKVLADVLGKNTDID
                     ILVEGHTDNVPIKTAVYKDNWDLSVARATSIVRILSEDYRIEPTRLTASGKGEFFPKA
                     DNATAEGRARNRRTEIVLSPKLEEIMQLLTN"
     gene            complement(9323..12457)
                     /locus_tag="PLZ75_03870"
     CDS             complement(9323..12457)
                     /locus_tag="PLZ75_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS71569.1"
                     /translation="MKTGNIFFLLLIIITAGLIKGCKQPAATRAGINDFDTIAAIFKS
                     PPAEFTTSPFFVWNYRITKEEIDRFMTDFSNSGSSQVFIHPRPGLITEYLSEEWLDLV
                     EYTVKKGRDLGMKVWLYDENSYPSGFAGGHVPDRMPESYNQGQGLLMKEFDILPDTAD
                     KFFLCLKEEDGKYIGITSGLENNRGKSGKYLLFHKTYYSRSGWFGDFSYVDLLCPGVT
                     QKFLEITMDTYERRIGYDFGKTVPGVFTDEPEISSPGGIRWTPDLFETFQKTWGYDLK
                     TNLPSLFRETGNWKKVRHNYTKTLLQMFIDRWAKPYYNYCEEKGLIFTGHYWEHLWPA
                     MNHGGDNMAMYAWFQMPGIDMLFNQFNDSSARAQFGNIRSVKELASAANQTGRQRKLS
                     ETYGGGGWELTFTDMKRLGDWEYALGVNFMNQHLSHYSLAGARKYDYPPTFSYHEPWW
                     GNYKYINDHYARLSMTLSAGKQINSILIIEPTTSAWLYDSYTARNPQCNTIGQSFQDF
                     ISILEKSQVEYDLGSEQIIKDLGSVKGRKLVVGQCSYKTVVLPPLTENIDLSTFKLLQ
                     KFVARGGRLIVFSTPSLVDGNADESLKSFFEKYADRISHEENLTADLITKYFKDQETN
                     FTGVSGGSLYHHRRVLADGQILFLVNSSLDENVIGSVSTDGADAIEMNTLTGEIYGYP
                     NKSEGNSVSITFNLPPAGSLLLYIPKNRKTDYDMPEVPEEFTRVPGASSMKIRRDYDN
                     AIAIPFCDLLLDGKLSRDLHNYTAADMVYKHYGFKDGNPWNHAVQFKTNITGRDKFDA
                     NSGFTAEYHFNIKGKFDYSSFRAVIERPYLWKVYINGTGIEPEENEWWLDREFAVFDI
                     SSYLIQGNNTITLKTAPVKVYAEIEPVYITGDFSVSPASKGWLIEAPSGSLRSGSWKD
                     QGMPFYSWTVTYSRDFNVEQQNSIYRVQLNDWKGTIAEVSVNGKWAGVIAFPPYNLNV
                     TKLIQPGTNTIDVKVTGSLKNLMGPHFNDPAPGMTGPGHWRNVKNYPDGRDYQLLDYG
                     LMEDFELLKIKTYL"
     gene            complement(12474..13907)
                     /locus_tag="PLZ75_03875"
     CDS             complement(12474..13907)
                     /locus_tag="PLZ75_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose isomerase"
                     /protein_id="HOS71570.1"
                     /translation="MREKYKLRIGLFGIGLDTYWPQFKGLKERLEAYLEEVCGKLKNF
                     NAYIVSLGLIDNPVSALEAGHKFRREDVDIIFLYVTTYALSSTVLPVVRRAKVPVIIL
                     NLAPEKAIDYQIFNNLNNRTAMTGEWLANVSACPVPEIANVFMRAGIRFHQITGVLHG
                     DEEVWKEVSEWVEAANVANILAHNRLGLMGHYYGGMLDVYTDLTLQLTSFGGHIEMLE
                     VDELSCLRRKISEQEIADRLELFYMEFDIDDGCAASDLRQAAITSVALEKMIEKHDLG
                     SLAYFYSGTGIQENEDSISTIILANSLLTSKGIPVAGEYEVRNVQAMKIMDSFGAGGS
                     FTEYYALDFNDDVVLMGHDGPGHLAIAEGKTKVRPLEVYHGKTGKGLSVEMSVRHGPV
                     TLLSVVQKPDGKLLLLVAEGESVPGPILEIGNTNSRYRFPGGMKNFVNAWNSHGPAHH
                     CAAGVGHIGRKIEKLGELLEMEVIKIC"
BASE COUNT         3449 a         3342 c         3255 g         4157 t
ORIGIN      
        1 caacaagggt ttttatccca tatgggagca tattgacacc acacttaagt atgcgatcag
       61 gtacctggaa aactaaatga cggcgcacac ttttttccat ttgcttaccc ttccgccggc
      121 attgtagatt tcgacagcca ggatatatat acccctcctg acaggcctgc cgtcatttcc
      181 gcagccgtcc cacaccagtg aggccctgaa tcctgccaga tggttttctg caagcttcct
      241 gacacagctg ccggtttcat caaatatcag cactgaaaca atgcttcccg ggtcatctga
      301 attaattcct atcaccagga catcctcgtg accgtcattg tccggggaaa ttttccgaga
      361 ggaaagtaca actgtttcag tgagattatc atctgcagta aatacggaat tcctgtttcc
      421 gggagtgccc cagcctgaac tgccggaagc cgagtgccag ctcgttcttt catcagaggg
      481 cagttccggc cttatcttct caagggagat gccctccttc cctgacagaa gggaataatg
      541 cattttctcg ctgtatatca cttcatcaat cctttcaagc tgcctgttca tcagcagcag
      601 atgacccctg gcatcgggca ttgagggaag tgcgggcact tcatggatat tttccccggc
      661 tgaaagggga aagcccgata tcaccttcag cctgtcggtt gtgatggtat agcaggttcc
      721 gggaatgatg catctaggtc cggtggaaat gctgagcgga tcagagattg cgccgctgcc
      781 ttccttcacg gacgagacca tgaactctga agcatcaaga attttccccg agatgttgac
      841 gaattctata taatcgggat tgtcggggag gggattgaaa agaagttcat tgaagacaag
      901 gtctttcgcg ccggcaggtt cagggattcc gaagcggaaa tcatacactg cgggacagtt
      961 gcctgcaaaa tccgatattt ccgcaggaag acccagggta tatatccttc ccctttcaag
     1021 gggaagaccg ggtataaggg taaagctgcg gtacagcgga tcggaaagtt cccagccggt
     1081 tacaggggat tccccgacgg tgatactgcc ggtctgattc ccgaaaccga tcagcggttc
     1141 actgaactca attattattt tcatgttgtc tgcaggaaaa acattggtaa taccccggaa
     1201 cgagttatcc gggtttgagc ggtgagccga gttgatcttt cccggagtgc ctcccttcct
     1261 gtcggatgag gcttcccagt tgccttcccc gaaaaaggga aaaccgggat caatgatttc
     1321 cagtgaccat cctcccttgc ttttcaggct gttgccgtac cagtttgagg aataatcgac
     1381 accattgata aggtttcccc ggtcgtcgta gagaagaacc agccttcccc tgtttgtcag
     1441 cgccgggaaa ggcttcagtc cggcaaccct gccgtatcct gtgaacagtc cggtatcact
     1501 gactgcgcat aaaataagat attccccggg ttcgatactt atttccggga aataggaatg
     1561 ctgggtggca gaggagagcc tccagtttgt cagatcaatt gtgtgccggc tccggttgag
     1621 gatttcaaga tattcctcac cgggaaggcc aagggaggga acgggatcag ccatgatttc
     1681 cgttatcacc acatcgcccg gcatgacccc cggtgaagac aggcagcatt ccggccgggc
     1741 cgggggaacg gagcggccct gtgccattaa gagcacatgg aaggccatca aaataacttt
     1801 tttcatgcag cgtgacgaat tgaaaaaaac cttatttttg accgtttttg cttaataaat
     1861 ttataaataa ttttacaaat cccaatcaag aaaatgaaag ttgctgtagt gggagcgacc
     1921 ggcatggtag gccggacgat gatgaatatt ctggaagaga gacagtttcc ccttactgaa
     1981 attattcctg ccgcatcgga gcggtctgcg ggaaaggaga tcactttcag gggaaggacg
     2041 gtaaagattg tcagcgtcag ggaagccgtg gacaggaaac ctgagtttgc aatattttct
     2101 gcaggagcat ccgtgtcgaa ggaatgggct cctgtgtttg cagggaacgg aacggtggtg
     2161 atagacaatt cctcggcatg gaggatggat aagaacattc cccttgtggt tccggaaata
     2221 aacagtcatg ttatccgcag gggcgacagg ataattgcaa atccgaactg ttcgaccaca
     2281 cagatggtta tggcactggc accccttcac aggaaataca ggatcagaag gattgttgtt
     2341 gcaacctatc agtcggttac cggaacaggt gtagaggctg tcaggcagat ggagaatgaa
     2401 aggaagggaa tcagcgggga aatggtttat cctcatccca ttgatctcaa ttgctttcct
     2461 cacggaggcg ccttcaagga ggacggatat accaccgagg agcagaagct tgttgatgaa
     2521 acacggaaga tacttgagga ccagtcaatc atggtgactg cgacggccgt gaggatacct
     2581 gttgtgggcg ggcattcgga agccgtgaat gttgaatttg aaaaggaatt cgacattgat
     2641 gaagtgaagg cccttatctc ggcatttccc ggcaatatca ttcatgatga ccctttccgt
     2701 aatgaatacc ccatgccgct gatggcgcac aaccgcgatg aagtgtttgt cggaaggata
     2761 agaagggatt tttcgaggga aaaatgcctc aacctgtggg ttgtttccga taatatccgg
     2821 aagggtgcgg caaccaatgc cattcagatt gccgagtaca tgctggagaa taagctttac
     2881 tagaattcag gatttcccct gctgtatagc tggtcagtat acagccggcc ttttagagtg
     2941 acgagatcag ttctgtcatt attattgtca ttttgtcttc tgcttttgca gcttccttta
     3001 gtactgcctc gtgggagttg tattcaatct ttccctccac accaaggtct gtgataatgc
     3061 ttaccgcgaa acagggaaga ttcatatggc gggccactat gacctcggtt gttgttgaca
     3121 ttcctactgc atccgcgccg gctgtccgga aaaacctgta ttctgccggt gtttcgaatg
     3181 taggaccggt tgttgagaga taaactccct tcctgaccct gatcccgtgt tccccggcaa
     3241 tctctgctgc cttcctgata agatacccgt cataggcttc ccccatgtcg gggaaacgga
     3301 cgccgatgcg atcgtcattg ggaccgatga gaggattggg cagaaggttg atatggtcgg
     3361 tgataatcat gatatcgcct atttcgaatg cgggattcaa tccgccggag gcattggaaa
     3421 ggaaaaggca tttgattcca agatatttca gcacccttac cgggaatgat actttttcag
     3481 gaccatggcc ttcgtagtag tgaaacctcc ctttcattgc cactactttc ctgttgccga
     3541 aatcgccgta gataagcttc ccgtcgtgcc cgtcgacagt tgataccggg aaattgggaa
     3601 tatccctgta atccatttct atgcagtttt ccatctttgc cgtcagcccc cccagtccgg
     3661 tgccgagtat gatgcccgca tcaggattga caaagcctcg ttctctgagg aaatcagttg
     3721 tttcgtaaat tttttccaac ataatctgtt attagcttat tgaattcttc ttttctgtga
     3781 tccggaaaat ctatttccac gggaatataa tacaacaccg gcagcagggg ttctgcaacg
     3841 gaacccgttt ccctcagcct gacagcatcc ttttcggtgg tgatgacata tttcagttcc
     3901 tccttctgtc cggtcagtat cttctgaatt ttccggatat cccccggacg gaaatagtga
     3961 tggtcgctga aacgcaggtg tatcaccctg ctgtacgatt tccgcagatg attataaagt
     4021 ggtgcgggat ttcctattcc tgtaagcaga aggatgccgt tatccggatc cccggaggaa
     4081 gggccgggaa gtgtattccc tttgaataca gggacaggat ccccgtaaac caggctggaa
     4141 aaaaacagct gttggcccgg agccaggtca aggctttccc tgatagcttt ctgctcctca
     4201 ggggagatgt cttcaggtgt tttcgtgatg attacaatat cggcccgctt caggtttccc
     4261 cgactttccc tcaggtttcc gtaggggaga agatgatcgc ggtacaccgg cctgtcataa
     4321 tccgaaagaa gtattgacag acccggcgtg actcttctgt gctggaatcc gtcgtcgagg
     4381 acaataacgc cggtttgtgg tctttcctcg agtattctgg ttatacctgc gcaccggtcg
     4441 cggtccactg caaccgttat atccgggaat tttctgctga taagaagggg ttcatcaccg
     4501 atatcggccg ttcctgaaga cggtccggcc agcaggaagc cctttgtttt tctcctgtat
     4561 ccccggctca gtaccgccac attgtacctg ctgcccagga gtcctgcaag gtatagggca
     4621 tgaggcgttt tgcccgtccc gcccactgta atgttaccga tgcatattac cggaaggctg
     4681 aatttccgtg acttcagtat ctttttgtca tacaggaagt ttctgaagcc agtaattatt
     4741 ccccagatga tggaaaaggg atacaggaat atgctccgtc tgttccccat ctatcagttt
     4801 atctcaataa gatatggcag gcgcgcctgt attcctgaaa gctcctttat ttccttcagt
     4861 tccctgtaga atctgactga ttccccgagt tcctttttca gaaatgcgga tctgatttct
     4921 gatccgagct ggctgatgag tttcatgtac gggtcttcgg caaccggcag ttcccttatt
     4981 gaatattttt cgattcctgc cagtgccgct gcacctttta tggcatcagt cagtcctccc
     5041 gtactgtcaa caagacccag tctcatggcg cttgaaccgc tccatacacg tccctgtgca
     5101 atattgtcga ctgattcgct gctcatcttc cttccctcac caacctttcc gatgaaatcg
     5161 ctgtatgttt tttcaacggc cgcctgcatt acctgtttct catagctggt catgggcctg
     5221 aaaaccgacg gaaaatcaga attctcattt gtattaaccg tttcagtgtt tatacccagt
     5281 ttctgttcca tcagttttcc tgtattggga aacaggccga aaacacctat tgatcctgaa
     5341 atagtggtcg gaccggcgta tatcttcgtt cccggtgcgg aaatataata tcctccggag
     5401 gctgcataat ttcccattga gattaccagg ggttttgttt ttgctgcaag atcgagttct
     5461 ctccacataa tatccgatgc tatagcactt cccccgcggg aatttactct caggacgata
     5521 gcttttaccg tgctgtcctt tctttccttc ctgatgatct ccgcaaagcg gtctccgcca
     5581 atgttgcttt catttccctt tccgtcggtg atgtttcccg atgcatagat tactgcaatt
     5641 cttgtctttg ttgtcagtac ctttttagga tccggaactt tagtgtactt gtgcattgga
     5701 accatggcta tcttgtcatc cgtacccagc cccgacagta ttttcagcgt atcgatcagc
     5761 tgatcgcggt atatcagtcc gtcaaccagt ccgctttcaa aagcatcatc tgcattgtat
     5821 cccgtgagtt tatcggcaag ggagttgacc cttccggttg tcatgccccg tgattcagct
     5881 atctttgctg ccacatgatt ccagatggat ccgacataat cttttatctg ctccctgttt
     5941 tcactgctga gcctgtcgag tatgtatggt tcaactgctc ccttgaattt tccgtgacgg
     6001 ataacctgca cttcaacgcc aagcttttca agagccttcc tgtaaaacat tatctctccg
     6061 ctcagtccct tgaaatcgag gactgatgcc gggtttatat agactttatc ggcagcggat
     6121 gagagataat aacctgcctg cggcaacagg tattcggaat aggcgattac aaatttattg
     6181 cttgtttccc tgaatttcag aagtgcattc cggatctctt ccgttgtggc ccatcctgaa
     6241 gccaggatgc cgttttcgat gagtatccct tttattttcc tgtcatttgc ggctttttcg
     6301 atgtttctca gaatatcatt cagtccggga gtcggggtaa ttgtcatgtt aaggacatcc
     6361 aggcctgcaa agggattggg gtcggttctg tcaggaacgg gaatgcctgt cttgagtacc
     6421 agtacagaat tatcggcaag tgatacactt ttgtttccgg aggcaattat aatgctcaga
     6481 ctggccgtca tgaccaggaa aaacaggatt gatgcaatta tgatacctgt tattgttgcc
     6541 agtacataaa caagaaaatt tttcattgca gggtgagttt aaaataaaga gaaccaaata
     6601 tatgaaaaac ctgaaaatta tgtaaagtta tcacaggatt taataaattt gaatgtaata
     6661 caggaggaat ggaaacagtt tttctcggtt taggaacaaa tctgggggag agggaagaaa
     6721 atctccggaa ggcggttttg cttattgaag aacatatagg gaagcctgtt gcggaatcac
     6781 ctgtatatga aacggaaccc tggggtttca cgagcgaaat aagtttcctc aacatggctg
     6841 ttaaggttga aacggatctg agcccctccg gtttgctggg aaggacactc atgattgagg
     6901 caatgatggg aagactgcgt gaaggcaagg gctacaagtc gagaataatt gatattgaca
     6961 ttttgctttt cggcagcagg gtaattgaaa ccagggccct gcagataccc catccgcgcc
     7021 tccatgaaag gaggtttgtt ctggttcctc tttcagggat tgcttcagat acagtccatc
     7081 ccgtactagg gaaaagcatt ggtgaattgc tggctgaatg cagggacggg aacaaggtgg
     7141 ctgagtacag aaaaaataat ctgtgatagt cagacttcac aacaatccgt tgtcagcaaa
     7201 gctgaaatat tctttttcag agattatcag atgatccagg agctggacat ccatgtaact
     7261 gccggcttcc ctgatttttt tggtgatttt tgtgtcggcc tcggaggggt tcaggtttcc
     7321 tgacggatgg ttatggcaca gaattattcc cgaagccagg cattcaacgg cttttttcag
     7381 tatcagcctt acgtcaatga ccgttccgct gatacctccc tggctgagtt tcattctgct
     7441 gatcaccttg tttgatctgt taaggtacag tatccagaat tcctcatggg caagatcaga
     7501 cagtatcggc tggaagatat ccgccacatc ctttgaagat tttataagga caaagctctc
     7561 tgcctccgcc agttttcttc ttcttcccag ttcaagtgcg gcagcaatgg ttacagctct
     7621 tgcaggaccg ataccattaa gctttgtaaa atctgaaatt gttagtttcc ccagattgtt
     7681 cagattgttt tttgccatgc tgaggagttc cctgcccaga tcgacagcag atttgtccct
     7741 tgtgcccgag cttatcagaa tacccaataa ttcggcattg cttaaactgg aagtaccttt
     7801 gtgaataagc ttttccctgg gtctgtcctc ggcagcccag tcagtaattt ttagcgacat
     7861 acagcgataa tttttattcc ttatgcaata taggaaatta cggcggaaat aaaaaaggcc
     7921 gtcaatactg acggcccggg aaatatttgt ctgattttat ttcaggctgt ttacatgttt
     7981 tgcgacagaa gatttgaggt ttgctgcctt gctgtggtga atgacattct ttttggccag
     8041 cttgtcgagc atagaattca tttttggcag taactccgaa gcctcggttt tgtctgtgga
     8101 agtacgtatg gttttcagtg cattacgcat tgtttttgca taatacctgt ttctggcatt
     8161 cctggtttct gtctgcctta tccttttttt cgctgatttg tgatatgcca ttttcttctg
     8221 cggttctgtt ttagcggggc aaagataatt atatttttat aaaatggaaa cgacagggaa
     8281 aaattttagt ttgtcagcag ctgcatgatc tcttcaagtt tgggagaaag aactatttcg
     8341 gtacgcctgt tcctggccct gccttcagct gtggcattat ctgccttcgg aaaaaattca
     8401 cccttgcctg aagccgtaag gcgtgtgggt tcaatcctgt agtcttcaga caatatcctg
     8461 acaatggacg ttgcccttgc aacactcaga tcccagttat ccttgtaaac agcagtttta
     8521 atgggcacat tatccgtatg tccttcaaca agtatatcaa tatccgtatt cttcccgaga
     8581 acatctgcca ggactttcag ggcttcacgt ccctttgact cgaccgcggc gctgccggat
     8641 ctgaacagga gcttgtcaga catggaaaca tacactttcc cgttctttat ttccagggat
     8701 agttcatccg gattaaatcc gagcagggca ttgtgcagga tactgttaag cctttttgtg
     8761 atagaatcct gcctggcaat gattttctgc atctcccgga gtgtttcctc cctttcggaa
     8821 agcagccttt ccttcctgtc gagctcggct gactttgcca tcagtgcctg gttaagctgt
     8881 tcggtccggg acaggctgtt atcgataaga gtattgtatt tttcctgcaa ttccctgttg
     8941 gctgccagct ggtcgttata ctggttccgt acctcattat gcattttcgt catttcggcc
     9001 aggctgcttg tcagtccggc attctcggcc tccttaagtt ccagttcggc acaaactata
     9061 tctctttccc gtaccacgtc cctcaggttt ttactccagg taaggggggt ctttgcaggt
     9121 ctcggctccc cgcatctgta aacaggtgca caggaagcgg ccagcattgc aattaatacc
     9181 ggcagtgcag catagctgat ctttctcatc tcatgtggtt tttaatgaat taactgtcgc
     9241 aacatcaatt tcagacaatc tgttttcaaa gatagatttt cttttgattc aacgcatttt
     9301 taaaggaaat cattgtgacg gtttacaagt aagtttttat cttcaacaat tcaaaatcct
     9361 ccattagtcc ataatcaagg agctggtaat ccctgccgtc cggataattt tttacattgc
     9421 gccagtggcc ggggccggtc atgccgggag caggatcgtt gaaatggggg cccatcagat
     9481 tcttaagact tccggtgact tttacatcaa tggtatttgt tcctggctga ataagtttcg
     9541 ttacattcag gttataggga ggaaaagcaa tgacaccggc ccatttcccg ttgaccgata
     9601 cttcagctat cgtccctttc cagtcattaa gctgaaccct gtaaatggaa ttctgctgtt
     9661 caacattaaa atccctgctg tatgtgactg tccatgaata gaacggcatt ccctgatctt
     9721 tccagcttcc ggaccttaag cttcctgacg gcgcttcaat cagccatcct tttgacgcgg
     9781 ggcttaccga aaaatcacct gtaatataaa ccggttcaat ctcggcataa accttcaccg
     9841 gagcggtttt cagggttatt gtattattcc cctgtatcag ataactgctt atatcaaaaa
     9901 cggcaaactc ccggtccagc caccattcat tctcctccgg ctcaattccg gttccgttga
     9961 tataaacttt ccacagatat ggtctttcaa taactgccct gaaggaagaa tagtcaaact
    10021 tcccttttat gttgaaatga tattctgcag taaagcctga attcgcgtca aatttatcgc
    10081 ggccggtgat atttgtttta aactgaacag catgattcca gggattcccg tctttgaaac
    10141 catagtgttt ataaaccatg tcggctgcag tataattatg gagatccctg ctcagtttcc
    10201 catccaggag cagatcgcag aatggaatcg cgatggcatt gtcataatcc cttctgatct
    10261 tcatggatga tgctcccggc acccgggtga attcttcagg aacttccggc atgtcatagt
    10321 ctgttttcct gtttttcgga atatacagaa gaagacttcc cgcgggaggg aggttaaatg
    10381 taatgctgac tgaatttcct tcggacttgt taggatatcc gtatatttca cccgtgaggg
    10441 tattcatttc tatggcatca gcaccatcgg tgcttaccga accaatgaca ttttcatcaa
    10501 ggctggaatt gaccaggaac aggatttgtc cgtcggcaag gactctcctg tggtgataca
    10561 gactgcctcc ggatacgccc gtaaaatttg tttcctgatc tttaaaatac tttgtaataa
    10621 ggtctgctgt cagattttcc tcatgactga tcctgtctgc atatttttca aaaaagcttt
    10681 tcaggctttc atcagcgttt ccgtcaacca gtgaaggggt ggaaaataca atcagccttc
    10741 ctccccgggc aacgaatttc tgcaggagct taaaggttga aagatcaata ttctccgtca
    10801 gagggggtaa taccactgtt ttataactgc actgtcccac taccagtttt ctgcctttga
    10861 ctgatcccag atccttgatt atctgttctg atcccaggtc atattcaacc tggctttttt
    10921 cgaggatgct tataaaatcc tggaaagatt gccctattgt attgcattga ggattcctcg
    10981 cggtgtagga atcatagagc catgccgatg tagtaggttc gatgatcagg atactgttta
    11041 tctgctttcc tgctgaaagt gtcattgaaa gccttgcata atgatcattg atatacttgt
    11101 aattacccca ccagggttca tgatatgaga atgtgggagg gtagtcatat tttcttgctc
    11161 cggcaaggct gtagtgggag agatgctgat tcataaaatt aacacccagt gcatattccc
    11221 agtcgccaag gcgtttcatg tcggtaaagg tcagttccca gccaccgccg ccataggttt
    11281 cactcagttt tctttggcgc cctgtctggt ttgctgcact ggccagctcc ttaactgacc
    11341 tgatgttccc gaactgggct cttgctgaag agtcgttgaa ctgattgaaa agcatatcta
    11401 tccccggcat ctgaaaccag gcatacattg ccatattatc tccgccatga ttcatggccg
    11461 gccataagtg ttcccagtaa tgaccggtga atatcagacc tttttcttca caataattat
    11521 aatatggttt ggcccatctg tcgatgaaca tctgaagcag ggttttggta taattatgtc
    11581 ttactttttt ccagttcccg gtttccctga acagtgatgg aagattagtc ttcagatcat
    11641 atccccaggt tttctgaaaa gtttcaaaaa gatccggcgt ccatcggata cctcccgggg
    11701 aactgatttc cggttcatcg gtaaaaacgc cgggaacagt tttgccgaaa tcatacccga
    11761 ttcttcgttc ataggtatcc attgtaattt ccaggaattt ttgtgtcaca cccgggcata
    11821 acaggtcaac atacgagaaa tctccaaacc agccgctcct gctgtaatat gttttatgga
    11881 aaagaagata ttttccgctt ttccccctgt tattttcaag acctgaagtg ataccaatat
    11941 attttccatc ttcttcttta aggcacagga aaaacttatc cgctgtatct ggcaggatat
    12001 caaattcttt cataagcaat ccctgtccct ggttatagga ttcgggcatc cggtcaggaa
    12061 catggccacc tgcaaatccg ctcgggtatg aattttcatc atagagccag actttcatcc
    12121 cgaggtctct gccttttttc acggtatatt caaccagatc gagccattct tccgacaggt
    12181 attcggtaat gagccccgga cgcggatgaa taaatacctg actgcttccc gagttactga
    12241 aatcggtcat gaatctgtca atctcctctt ttgtgatcct gtaattccat acgaagaaag
    12301 gagaggttgt aaattccgcg ggtggtgatt taaagatagc ggcgattgta tcgaaatcat
    12361 tgattcccgc tctggtcgcg gctggttgtt tacacccttt gatcaggcca gccgttataa
    12421 taattagtaa taagaaaaag atgtttcccg ttttcatgat cctgataatt ttattaacag
    12481 atttttataa cttccatttc aagtaattcg ccgagttttt ctattttccg gccgatatgt
    12541 ccgacaccgg ctgcacaatg atgtgccggg ccatggctgt tccaggcgtt gacgaaattt
    12601 ttcatcccgc ccgggaatct gtaccggctg ttggtattgc caatttcaag aatcgggccc
    12661 gggactgact cgccttcagc caccagcaga agaagctttc catcaggctt ctgtactaca
    12721 gaaagaagtg ttaccggtcc gtgcctgaca gacatttcaa ctgaaagacc cttacctgtt
    12781 ttcccatgat atacttcaag aggcctgact tttgtttttc cttcggcaat tgccagatgt
    12841 ccggggccat catgtcccat cagtaccacg tcatcattaa aatcgagtgc ataatattcc
    12901 gtgaatgatc ctcccgcgcc aaaactgtcc attattttca tggcctggac attcctgacc
    12961 tcatattcgc ctgctaccgg tattcccttt gatgtgagca atgaattggc aagtatgatt
    13021 gtacttattg aatcttcatt ttcctgtatt cccgtgccgc tgtagaaata tgccagtgaa
    13081 cccagatcat gtttttcaat cattttttcc agggcaactg atgttattgc agcctgtctg
    13141 aggtcagatg cggcacaacc gtcatcaata tcgaattcca tgtaaaacag ttcaagacgg
    13201 tcggcgattt cctgttcgga aatttttctt ctgagacatg agagttcatc gacttcaagc
    13261 atttcaatgt gaccgccgaa ggaggtcagc tggagggtaa ggtccgtgta aacatcgagc
    13321 attccgccat aataatgacc cattaaacca agccggttat gtgccagtat attggcaaca
    13381 tttgctgcct caacccattc actaacctct ttccatacct cctcgtcacc atgaagaacg
    13441 ccggttatct ggtgaaatct gatacctgct ctcatgaaga cattcgctat ttcgggaacc
    13501 ggacaggcag agacatttgc aagccattcc ccggtcattg cggtccggtt attcaaatta
    13561 ttaaagattt gataatctat ggctttctcc ggagcaagat taagaataat cacaggtacc
    13621 ttggctctcc ggactaccgg gagtaccgtg gaggaaagag cataggtggt aacatataag
    13681 aatattatat caacatcctc tcttctgaat ttatgtcctg cctccagggc gctgaccgga
    13741 ttatcgatca gtccgagact gacaatataa gcattgaagt ttttcagttt tccgcagact
    13801 tcttccagat atgcttcgag gcgttccttc aatcctttga actggggcca gtaggtatca
    13861 agtccgatcc cgaaaaggcc gattcttagt ttgtatttct ctctcatggt taattacagg
    13921 gttttaatgt tcagtttttc acggagtttc aaagtttagg ggttcaggag ttacagagta
    13981 ttgctctcac tcctcatggc aatttacaaa ttattggcgt tagtgcggta aaaaccaatt
    14041 ttctgattat tttatcattt atgatcaggg tgttacaaat aaatgcagaa ttaattttgc
    14101 agagcgtcac ttgtcgggaa atactgtcag ctgcgttaag gaattatagt ccgcccggag
    14161 aatttttatt tgacttgggg tttcgtattt cgtaacttta aca
//
LOCUS       DAOSPX010000046        14199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4355_length_14199_cov_4.984446, whole genome shotgun sequence.
ACCESSION   DAOSPX010000046 DAOSPX010000000
VERSION     DAOSPX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14199
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4355_length_14199_cov_4.984446"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PLZ75_03880"
     CDS             complement(<1..1272)
                     /locus_tag="PLZ75_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOS71571.1"
                     /translation="MKRGVTVLIVSLIFSFCHAQRKMTEPVLPPHYVKTINSHWTFNY
                     FPDATEGSGYESPLYDDSNWPAISIPHTWNSYETTFELHSSAEGNTGGTDNPRWREGR
                     GWYRKHFIINPAAAGKKIFIEFDGVQKYCKLWLNGKYLGDHDGFHTSFVFDITEHTVT
                     GKDNVLAVAVSNSGRNQAAGSAAEENRFHMFGGIYRDVRLIITDRLYITLKEAASPEK
                     GIITGTPAISERDGVVSIRASVRNDYPDTRKCIVKTCITDRSGRNVQVLKSRVSIAPG
                     RLYGFEQLSRPIAGPQLWSPGSPALYGVRTEVTSGGKVTDVYNSTLAFKRFEWNSDDR
                     SLLLNDEKIVLRGGRLNPVHPWLGIAIPRWMREAEIRNMAENLGYNYLVTGIVPADRF
                     LYELADRYGIIIIDEEFSTAQAAVETMAGSRD"
     gene            1686..3512
                     /gene="ilvD"
                     /locus_tag="PLZ75_03885"
     CDS             1686..3512
                     /gene="ilvD"
                     /locus_tag="PLZ75_03885"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOS71572.1"
                     /translation="MRNKLRSSTTMTGRTMAGARSLWRANGMKESQFGKPVIAIVNSF
                     TQFVPGHVHLHGAGQMVKEEIEKHGYFGAEFNTIAIDDGIAMGHTGMLYSLPSRELIA
                     DSVEYMCNAHCADAMICISNCDKITPGMMMAAMRLNIPVVFVSGGPMEAGVYKDRQID
                     LIDAMIDGGDENVSDEELLEIERSACPTCGSCSGMFTANSMNCLNEALGLALPGNGTV
                     LATHANRLRLIKKAANLIVSLADRYYFEGDGSVLPRSIATREAFMNAMALDIAMGGST
                     NTVLHLLAIAREAEVDFTMKDIDELSRRIPNICKVAPSSSYHVQDVNRAGGIMAIMGE
                     LSRNNLIDTSVKRVDYPTLRQALDDWDISGGSAGPGAEDLYRSAPSMKRNIVLGSGES
                     MYKSLDTDREKGCIRSVANAYSIDGGLAVLYGNIAEDGCIVKTAGVDTELLRFRGPAI
                     VFESQEDAVEGILNGTVKSGDAVVIRYEGPKGGPGMQEMLYPTSFLKSRGLGRECALI
                     TDGRFSGGTSGLSVGHTSPEAAAGGAIAIIENGDIIAIDIPGRKINVELTVKEIESRL
                     QKECSRKDAAFMPRNRERKISASLRAYASIVSSADKGAVRIV"
     gene            3590..5335
                     /gene="ilvB"
                     /locus_tag="PLZ75_03890"
     CDS             3590..5335
                     /gene="ilvB"
                     /locus_tag="PLZ75_03890"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOS71573.1"
                     /translation="MMTDKEVQETMERINGTEEITGAEALLRCLIEEGVDTIFGYPGG
                     AIMPVYDKLMDYDHKLKHILTRHEQGSAHAAQAYAMVTGKPGVCFSTSGPGATNLVTG
                     IANAFLDSVPMILVTAQVVSGLIGTDAFQETDILGVSMPITKWNCQIKNAQDIPGVLA
                     KAFYIATTGRPGPVLIDIAKDAQINKLQFRYEKCTYLRSYNPNVPVNMKAIESAAIMI
                     NHAQKPLILAGHGVLLARAENELLRFAEKTGIPVAVTLLGLSAFPSGHRQYAGFLGMH
                     GNYAPNLLTNEADLIIAIGMRFADRVTGNLNDYAKKARVIHIEIDQAEINKNIQVDIE
                     IHSDAKIALQNLTAMVRENSYESWIREFRILDNIEYDRVIKEETKPASGRMRMGEVVK
                     LVSDMTNGDAIIVTDVGQNQMSAARYYRFSNPNSFVTSGGMGTMGFGLPAAIGAKIGA
                     PDKEVIAFLGDGGFQMTIQELGTILQYKVPVKLVVLNNEFLGMVRQWQDMFFEKRYAA
                     TELVNPDFIGIAAAYGIPAKKITEREDLEESLKEMLEAEGPFILETFIEKEGNVLPMI
                     EPGASVSQVTLNYKG"
     gene            5461..6018
                     /gene="ilvN"
                     /locus_tag="PLZ75_03895"
     CDS             5461..6018
                     /gene="ilvN"
                     /locus_tag="PLZ75_03895"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOS71574.1"
                     /translation="MKQEFTISIFSENHIGILNQITIILTRRQVNIESLTASESAVKG
                     VHLLTLVVEVTPDMVRKISRQIEKLVEVLKVFVHTSDQIIYQEIALFKVSAKSIMTGD
                     ILERMVKHYNARILVVSPEYIVIEKTGHKSEINELLEKLETYGVLQFARSGRVAITKQ
                     VKELTHYLKELDEASRKTPGEPVLN"
     gene            6102..7151
                     /gene="ilvC"
                     /locus_tag="PLZ75_03900"
     CDS             6102..7151
                     /gene="ilvC"
                     /locus_tag="PLZ75_03900"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOS71575.1"
                     /translation="MAKIDFGGVIENVVTREEFPLSKAREILKDEIVAVIGYGVQGPA
                     QALNMRDNGINVIIGQAPEFKADWDKAVKDGFVPGKTLFPVEEAAEKGTIIQYLVSDA
                     AQRILWPKIKPCLKKGDALYFSHGFSVTYKDHTGVVPPENVDVIMVAPKGSGRSVRLN
                     YLAGTGINSSYSVFQDFTGKAEERTIALGIAIGSGYLFPTTFENEVYSDLAGERGVLM
                     GALAGIMEAQYEVFRENGHSPSEAFNETVEELTQSLIRLVDENGMDWMYSNCSATAQR
                     GALDWKPRFREITLPLFRELYEKVKSGEECERVLKSTGVPNYKEILDGELREMADSEL
                     WKAGAAVRKLRPETK"
     gene            7176..8669
                     /locus_tag="PLZ75_03905"
     CDS             7176..8669
                     /locus_tag="PLZ75_03905"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOS71576.1"
                     /translation="MTEKVIIFDTTLRDGEQVPGCQLNTIEKIQVAKALEELGVDVLE
                     AGFPISSPGDFNSVVEISKAVSNPVICALTRAVKKDIEVAAESLQFAKKKRIHTGIGV
                     SDYHMRYKIKATPEEILRRAADAVKYAKKFVEDVEFYAEDAGRSENEFLARVIEAVID
                     AGATVVNIPDTTGYCMPLEYGSKIRYLKENVRNIDKAIISTHCHNDLGMATANTMMGV
                     LNGARQVEVTINGIGERAGNTSLEEVAMIIKSHHDLNLETSINSTLIYKTSRLVSNLM
                     SMPVQANKAIVGRNAFAHSSGIHQDGVLKKRENYEIIDPVSVGVRESSIILTARSGRA
                     ALNHRLEMLGFSYGKEELDDIYHRFLLLADKKKEINDEDIRILTGQVFQGDKSVKLEH
                     LQVICGKSAIPVATVGLNINGEIHTATSVGNGPVDAAFTAVKQLISKTVHLEEFLIQA
                     ITQGSDDLGKVHVQLEHDEKIYYGFGTSTDIVTASVEAFIDAITKIK"
     gene            8748..10154
                     /gene="leuC"
                     /locus_tag="PLZ75_03910"
     CDS             8748..10154
                     /gene="leuC"
                     /locus_tag="PLZ75_03910"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOS71577.1"
                     /translation="MKPETLFDKIWDSHVVRSIEGGPDVLYIDQHFIHEVTSPQAFDG
                     LRARGIDVFRPSMTLATADHNIPTVNQNMPIRDELSRRQVETLTRNCNEFGITCYGLN
                     DPHNGIVHVIGPELGYTLPGMTIVCGDSHTSTHGAFGTVAFGIGTSEVEMVLATQCIL
                     QPKPKKMRIRVEGELGRGITAKDLILYIISQLSTSGATGYFVEYAGSAVESLSMEGRM
                     TVCNMSIEMGARGGLIAPDDTTFEYLRKIPALSNRPDFYALVKKWSRLRSDPDAVFDR
                     ELTFDAGKIKPMITYGTNPGMGISIDESIPALEEISDETKASFIKSLNYMGFTPGMKL
                     AGQPVDYVFLGSCTNGRIEDLRAFAGFVRGRKKAPGVTVLIVPGSKKVEKQAIDEGLA
                     DILKDAGFVLRQPGCSSCLAMNEDKIPPGKYAVSTSNRNFEGRQGPGARTLLASPLTA
                     AAAAVTGRITDPGELMQD"
     gene            10212..10814
                     /gene="leuD"
                     /locus_tag="PLZ75_03915"
     CDS             10212..10814
                     /gene="leuD"
                     /locus_tag="PLZ75_03915"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOS71578.1"
                     /translation="MPKEKFDILESTCIPLPIENIDTDQIIPARFLSLTSREGFGEYL
                     FRDWRFDKSGNKIEDCVFNNPRYSGRILVTGKNFGSGSSREHAVWAIAGYGFRAVVSS
                     FFADIFRNNCLNNGILPVVVKEDYLQNLLKTIRDNPSTMARIDLDKQLFTVLPDGEGI
                     SFEINPYKKECLLNGLDDIDYLLSIQDAIKAYELRKTLGK"
     gene            10826..12358
                     /locus_tag="PLZ75_03920"
     CDS             10826..12358
                     /locus_tag="PLZ75_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOS71579.1"
                     /translation="MDTTLRDGEQTQGVSYSPFEKLHLAKFLLKDLRIDRIEVASARV
                     SKGEFEAIRQITEWAARNDFHNKVEVLGFIDGETSLNWIRDAGARVINLLCKGSLRHL
                     EKQLRKTPETHAADIRDIIRKTVDSGMEINIYLEDWSNGMIHSEDYVFFMIDSIRDEP
                     VKRIMLPDTLGILNPDQTYSFCKKIVDTYPGIHFDFHPHNDYDLAVANVFSAVKAGIR
                     GVHTTINGMGERAGNAPLSSVIGVLHDQLEAETGINEMEITMASRIVETFSGIRVPPN
                     KPVIGENVFTQTSGVHADGDSKDNLYFNNLLPERFGRKRKYALGKQSGKATIRKNLEE
                     FGLALSAESIDKITQRVIELGDKKESVTTEDLPFIISDILGTEQIVYRILIKNYALST
                     SYGLKPFASVKMEIDGQIHEGTSAGDGQYDAFMRAVRMIYEKVGKELPHLTDYQVSIP
                     PGGKTDALVETIISWKHHDKEFRTKGLDVDQTGSAIKATEKMLNLIESLNNGTEIKTM
                     PV"
     gene            12401..13462
                     /locus_tag="PLZ75_03925"
     CDS             12401..13462
                     /locus_tag="PLZ75_03925"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOS71580.1"
                     /translation="MNKNYNIAVIPGDGTGPEVVREGKKVLLAAASRFGFSIGFGEYD
                     FGGDRYLRTGEILPDSAVEELKKYDSIFLGAIGHPGVRPGILEKGILLRLRFELDQYI
                     NLRPVKLYPGVDTPLKDKGPEHIDYVVIRENSGDVYTGSGGVTMKGTAHEIAIQNMVY
                     SRMQVDRCLEYAFEYTRKRNRKKTLALCGKTNVLTYVFDLWERAFHEIGEAKYPDIKR
                     EYYHVDAISMWMIKNPEWFDVVVTSNMFGDIITDLGAITQGGMGVAAGGNINPEGVSM
                     FEPIGGSAPKYTGLNVINPIAAISCGAMMLDHLGEGKAANAIENAIMDVTANKLKDMG
                     AGRMGYSTSEVGDLVAERI"
     gene            13749..>14199
                     /gene="hisG"
                     /locus_tag="PLZ75_03930"
     CDS             13749..>14199
                     /gene="hisG"
                     /locus_tag="PLZ75_03930"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOS71581.1"
                     /translation="MNRLKIAIQKSGRLNEKSRSLLAECGIKFPNGTGLLKTSARNFP
                     VDILFLRDDDIPQYVEQQVADIGILGENVVYEKNRNVSTLEKLGFANCRLSLAIPGED
                     RFTGPEYFNNKRIATSHPRLLSEYLSANSISARIEEISGSVEIAPGIG"
BASE COUNT         4251 a         3192 c         3551 g         3205 t
ORIGIN      
        1 atctctgctg ccagccatgg tttcaacagc tgcctgggcc gttgaaaatt cctcatcaat
       61 aataatgata ccgtacctgt cggccagctc atataaaaac ctgtcggcag gaacgattcc
      121 cgtcaccaga tagttatatc cgaggttctc agccatgttc ctgatttccg cttccctcat
      181 ccaccgggga attgcaatgc ccagccaggg atgaacaggg ttcaggcggc ctcccctgag
      241 aacaattttt tcatcattca gcaagaggct tctgtcatcg gaattccatt cgaacctttt
      301 aaatgccagg gtgctgttat aaacatctgt aactttcccc ccgcttgtca cttccgttct
      361 cacaccatac agagcgggtg atccgggaga ccacagttgt ggtcctgcaa tgggccggga
      421 cagctgttca aagccgtaca gacggcccgg agcgatactc acccttgact tcagcacctg
      481 cacattcctt ccggatctgt cggttataca ggttttgaca atgcatttcc gggtatccgg
      541 ataatcattc ctgaccgatg cccggatgct tacaactccg tccctttcac ttattgccgg
      601 tgtgcctgtt attataccct tctcagggga agccgcctcc ttcagtgtta tatacagacg
      661 gtccgtgata atcagccgga catcacggta tataccgcca aacatatgaa atctgttttc
      721 ttcagccgca gaaccggcag cctgattcct gccggaattg ctgacagcta ctgccaggac
      781 attatccttt cctgttactg tatgctcagt tatatcgaaa acaaatgatg tgtggaatcc
      841 gtcgtgatca ccaaggtatt ttccgttcag ccacagttta cagtatttct gaaccccgtc
      901 aaactcaatg aagatctttt ttcctgctgc cgccgggttg atgatgaaat gtttccggta
      961 ccagccccgg ccctcacgcc accgcgggtt atcagttccg ccggtattcc cctccgcgga
     1021 agaatgcagt tcaaaggttg tttcgtagct gttccatgta tggggaatgc ttattgccgg
     1081 ccagttcgaa tcgtcataca ggggagattc atatccgctg ccttccgttg catcaggaaa
     1141 atagttgaaa gtccagtgtg agtttatggt ctttacataa tgcggcggaa ggacgggctc
     1201 agtcattttc cgctgggcat gacaaaatga aaatatcagt gatacaatca gaaccgtgac
     1261 acctcgtttc atattgcaga ctttcggagt gataaagtta ataattgttg gataaactgc
     1321 aacatcaccc gtatgttaat aaacggaaag aaatcaacta ttttatttca gatgttgctg
     1381 ctgcttaccg caaaagttaa tttattgatt tactgtacgt ttttttaaag ttttgctgag
     1441 aagcgggaca gggaatataa aattttcaag aaaaaatact tttttctttg atatattgaa
     1501 ataataagta cttttgtcag tctcataaaa acgaaaccat gagaaccgga ttaatacata
     1561 ttatcgccct cattcttatt ctcgtgcgct gcgcatgatc cgggaataat atgtattaac
     1621 acaagaatat acagaagctt tccggatcag tccgggaagc ttttttttgt caaaaattat
     1681 ctgaaatgag aaataaactc agaagttcaa caacgatgac cggccgcacg atggccggtg
     1741 cccggagcct ctggcgtgca aacgggatga aggaaagcca gttcggcaaa ccggtaatag
     1801 ccatagtgaa ctcattcaca cagtttgtgc cgggacatgt tcacctgcac ggagccggtc
     1861 agatggtgaa agaggaaatt gaaaaacatg gctattttgg tgccgaattc aacaccatcg
     1921 ccattgacga cggcatagca atgggccata ccggaatgct ctactccctt ccgtcgaggg
     1981 aactgattgc cgacagcgtc gagtacatgt gcaatgcgca ttgtgcggac gcaatgatct
     2041 gcataagcaa ttgtgacaaa ataactcccg gcatgatgat ggccgcaatg aggctaaaca
     2101 tcccggtggt attcgtttca ggcggaccca tggaagcagg agtttacaaa gacaggcaga
     2161 tcgacctcat cgatgcaatg attgacggcg gtgatgagaa tgtgagtgat gaagaactcc
     2221 tggaaattga aaggtcagcc tgccccacct gcggatcctg ctcgggaatg ttcactgcca
     2281 attcaatgaa ttgcctgaat gaagcccttg gccttgccct tccgggaaac ggaacggttc
     2341 tggctactca tgccaaccgg ctgagactca tcaaaaaggc tgcgaatctg atcgtttcgc
     2401 ttgcagacag atactatttt gaaggtgacg gttcagtgct tccgcgttca atagcgacac
     2461 gggaagcctt catgaatgcc atggcgctcg acatagccat gggaggctcc acgaacaccg
     2521 ttctgcatct gctggcaata gcccgggaag cagaagtgga cttcacaatg aaagatatag
     2581 atgaactttc acgccgtatc cccaacatat gcaaagttgc ccccagctca agctaccatg
     2641 tccaggatgt taacagggcc ggcggaatca tggcaataat gggtgaactc agcaggaata
     2701 acctgattga tacttcagta aagcgggttg attatcccac actcagacag gcacttgacg
     2761 actgggacat aagcggtggc agtgccggtc ccggggcaga ggatctgtac aggagcgcac
     2821 cgtcgatgaa aagaaatata gttttgggat ccggtgaaag tatgtacaaa tcacttgaca
     2881 ccgacaggga aaagggatgt ataaggagtg tggccaatgc ctacagtatt gacggcggtc
     2941 tggcagtttt atacgggaat atagctgagg acggctgtat tgtcaaaaca gcgggtgtcg
     3001 atacggaact tctcagattc agaggacctg ccattgtttt tgaatcccag gaggatgccg
     3061 ttgaagggat tctgaacgga acggtaaaat caggagatgc cgtggtgatc aggtatgaag
     3121 gtccgaaagg cggaccggga atgcaggaaa tgctgtatcc cacttctttc ctcaagtcaa
     3181 ggggactggg cagggaatgt gcgctgatta ccgacggaag gttttcggga ggcacctcgg
     3241 gcctttcggt gggacataca tctcccgagg cggcagccgg aggcgcaatt gccataatag
     3301 aaaacgggga tataatagca atagacatcc ccggaaggaa aatcaatgtt gaacttacag
     3361 taaaggaaat agaatcgaga ctgcaaaaag agtgttcgag aaaagatgca gccttcatgc
     3421 cccggaacag agaaaggaag atatccgcct cactgagggc ttatgcttca atagtgagct
     3481 cagcggataa gggagcagtg agaattgtct gaaaacatac cggagcccga aagctggaaa
     3541 aagctaaagt tagaaagtaa aatatttttc gttttaaaag gacaaaacta tgatgactga
     3601 taaggaagtc caggagacaa tggaaagaat aaacggaacc gaggaaatta ccggagcaga
     3661 ggctctgctg agatgcctca tcgaagaggg tgttgacacc attttcggat atccgggggg
     3721 agctatcatg ccggtatatg acaagctgat ggattacgac cacaagctta agcatatcct
     3781 cacaaggcat gaacagggct ctgctcatgc tgcacaggca tatgccatgg ttacaggcaa
     3841 gcccggagtc tgcttttcaa catcgggacc cggagccaca aatcttgtta ccggaatagc
     3901 aaacgccttc cttgattccg tcccgatgat ccttgtcacc gcacaggtgg tgtcggggct
     3961 gataggaacc gatgcttttc aggaaaccga catactgggt gtatccatgc ccataacaaa
     4021 atggaactgc cagataaaaa acgcacagga catacccgga gtcctggcga aagcattcta
     4081 tatcgccacc acgggcagac ccgggccggt actgatcgat attgccaagg acgcacagat
     4141 taacaagctt cagttcagat acgagaaatg tacctatctg agaagctata atcccaatgt
     4201 tccggttaac atgaaagcga tagaatcggc agcgataatg ataaaccatg ctcaaaaacc
     4261 cctgattctt gcaggtcatg gagtgcttct cgcgagggcg gaaaatgaac ttctccgttt
     4321 tgcggaaaaa acagggatac cggttgcggt aaccctgctg ggcctgtcgg cttttccctc
     4381 cggacacagg caatacgcgg gattcctggg catgcacggc aactatgccc ccaacctgct
     4441 gacaaatgaa gctgatctga ttattgccat tggaatgcgt ttcgctgacc gtgttacggg
     4501 aaacctgaat gactatgcaa agaaagccag ggtgatccat atagaaatcg accaggctga
     4561 aataaacaag aatatccagg ttgatatcga aatacacagt gacgcaaaaa tagccctgca
     4621 gaatcttacc gccatggtaa gggaaaacag ttatgaatca tggatcaggg aattcaggat
     4681 actggacaat attgaatatg accgtgttat taaagaggaa acaaaaccgg cttccggccg
     4741 catgagaatg ggcgaggtag taaaacttgt ttccgacatg acaaacggag atgccataat
     4801 agtaaccgac gtgggccaga accagatgtc ggcagcaagg tattacaggt tcagcaatcc
     4861 caattccttt gttacctcgg gaggcatggg aacaatgggt ttcggactgc cggccgccat
     4921 aggtgcgaaa atcggcgccc cggacaagga ggttattgca ttcctgggcg acgggggctt
     4981 ccagatgaca atacaggaac tgggaaccat acttcagtat aaagtgcctg tcaaactggt
     5041 cgttctcaat aatgaattcc tcggaatggt aaggcagtgg caggatatgt ttttcgaaaa
     5101 aagatacgct gccaccgaac tggtcaatcc cgattttatc ggaattgccg cggcatacgg
     5161 cataccggcg aaaaagatca ccgaaagaga ggacctggaa gagtccctga aagaaatgct
     5221 cgaggcagaa ggccccttca tcctggaaac cttcattgaa aaggaaggaa acgttctgcc
     5281 gatgattgaa cccggagcct cagtctcgca ggtaacactt aattataaag gataaaaatc
     5341 tgaccaataa agggagagaa acttcggtac tgcatgcagc ccatgacatc agggtgatta
     5401 cctgagtaag ggcagggcag ccgagggaaa ccccggaaaa aatacgaaac agtaaaattt
     5461 atgaaacagg aatttaccat ttcaatcttc agtgagaacc atataggtat tctcaaccag
     5521 ataacaatta tcctgacacg gagacaggtg aacatagaaa gcctgactgc ttcggaatcg
     5581 gccgtaaaag gcgttcacct ccttaccctg gtggttgagg taacacccga catggtccgc
     5641 aagatttcac ggcagataga aaagctggtt gaggtgctga aagtgtttgt acacacgtcc
     5701 gatcagataa tttaccagga aatcgccctg ttcaaggtat cggccaaaag cattatgaca
     5761 ggcgatattc tggaaaggat ggttaaacac tataacgcca gaatactggt tgtttccccg
     5821 gaatatatag tgattgaaaa gacaggccac aagagcgaga taaacgagct gctcgaaaaa
     5881 ctggagacat acggggttct gcagtttgca cggtcgggaa gggtggcaat aacaaagcag
     5941 gtaaaggaac tgacccatta cctgaaggaa cttgacgagg caagccggaa aacacccggt
     6001 gagccggtcc ttaattaact gataaaataa ctcttctgtc ccccccggga acggaaaggg
     6061 ttactgcata atttaaaata ttaattaaaa aatcttgaaa aatggcaaaa attgattttg
     6121 gaggagttat tgagaatgta gttacaagag aagaattccc tttgtccaaa gcacgcgaaa
     6181 ttcttaaaga tgaaattgtt gcagtcatcg gatacggtgt tcagggtccg gctcaggcat
     6241 tgaacatgag agataatggt ataaacgtga tcataggcca ggcacctgaa ttcaaggctg
     6301 actgggacaa ggcagtaaaa gatggatttg ttccgggaaa aactttgttt ccggttgagg
     6361 aagcagcgga aaaaggcacc atcatccagt atcttgtatc ggatgccgcc cagcgtatat
     6421 tatggccaaa gatcaagccc tgcctgaaaa aaggtgatgc cctctacttt tcacacggat
     6481 tctccgttac gtacaaggac cataccggag ttgttcctcc ggaaaatgtt gacgtgataa
     6541 tggttgcgcc aaaaggctca ggcagaagcg tacgcctgaa ttatcttgca ggaaccggta
     6601 tcaactcaag ctattctgtt ttccaggact ttacgggaaa ggctgaagag cgtacaattg
     6661 ccctgggaat agccataggt tcaggatatc ttttccccac aacctttgaa aatgaagtat
     6721 acagcgatct tgcaggtgaa cgcggcgtgc ttatgggagc tctcgcggga ataatggaag
     6781 cccagtatga agtattcagg gaaaacggac acagtccctc cgaagctttc aacgaaaccg
     6841 tggaagaact gacccagagc cttatacgac tggtggacga gaacggtatg gactggatgt
     6901 attcaaactg cagtgccaca gcccagagag gagctcttga ctggaagccc cggttcaggg
     6961 aaatcaccct tccgcttttc agggaactgt atgaaaaagt gaaatccggc gaggaatgtg
     7021 aaagagtact gaaatcgaca ggagttccca attacaagga aatactggat ggcgaactca
     7081 gggagatggc tgattcggaa ttatggaagg caggagcagc cgtgaggaaa ttacggccgg
     7141 aaacaaaata aacaaaccaa cattttatta aatatatgac tgaaaaagta atcattttcg
     7201 acacaaccct gagggacgga gagcaggtgc cgggttgcca gctgaacacc attgagaaga
     7261 tacaggtagc caaagccctc gaggaactcg gggttgatgt gcttgaagcc ggttttccga
     7321 tctccagtcc gggagatttc aattcggtgg ttgagatatc caaagctgta tcaaatcctg
     7381 ttatctgcgc tctcacaagg gcagtaaaaa aggatattga agtggcagca gaatctcttc
     7441 agtttgctaa gaagaagagg atccataccg ggataggtgt ttcagactac catatgcgct
     7501 acaagatcaa ggcaactccg gaggagatac tcagaagggc tgccgatgcc gtgaagtacg
     7561 cgaagaaatt tgttgaggat gtggaattct atgctgaaga tgcgggccgc agtgaaaatg
     7621 agtttctggc aagagtcatt gaagccgtca ttgatgccgg ggcaacagtg gtgaatattc
     7681 ccgatacaac cggctattgc atgccactgg aatacggcag caaaataaga tatctgaagg
     7741 agaatgtaag gaacattgac aaggcaatca tatccaccca ttgtcacaac gatctcggaa
     7801 tggccacggc caataccatg atgggagtcc ttaacggggc cagacaggtt gaggtaacca
     7861 ttaacggcat aggggaaaga gcgggaaaca cctcactgga agaagtggca atgatcataa
     7921 agagccacca cgacctgaac ctggaaacca gcatcaattc cacacttatc tacaaaacca
     7981 gcaggctggt atcaaatctg atgagcatgc ccgtccaggc aaacaaggcc atagtgggaa
     8041 gaaacgcctt tgcccattcc tcagggatac atcaggacgg agtgctgaaa aaacgcgaga
     8101 actatgagat catcgatccc gtgtcagtgg gcgtgaggga atcttcaatc attcttaccg
     8161 cacgcagcgg aagggccgcc ctgaaccacc gcctggaaat gctcggtttc agttatggca
     8221 aggaggaact cgatgatatc taccaccgct tccttcttct tgcagataag aaaaaggaaa
     8281 tcaacgacga ggatatcagg atactgacag ggcaggtttt ccagggtgat aaatccgtta
     8341 aactggaaca tctccaggtc atctgtggca aatcagccat cccggtggca accgtgggtc
     8401 tgaatataaa cggggaaatc cacactgcca catccgtcgg aaacggcccg gtggatgcag
     8461 cattcactgc agtgaaacag cttatttcca aaacagttca cctggaggaa ttccttatac
     8521 aggcaatcac ccagggcagc gatgatctcg gaaaagtaca tgtacagctt gaacacgacg
     8581 agaaaatcta ttacggcttc ggcaccagca cggatattgt aactgcctca gtggaagcat
     8641 ttatcgatgc aataacaaaa atcaagtaag tatccggaat ctgtttggcc cgccggatga
     8701 caggcacact gagttttaag gaaaaacaga ctgcttagat aataaaaatg aaaccagaaa
     8761 cacttttcga caagatatgg gacagccatg ttgtcagatc catcgaagga ggaccggatg
     8821 tcctgtacat cgaccagcat ttcatacatg aggttaccag tccgcaggcg tttgacggac
     8881 tgagagccag gggcatagac gtgttccgcc cttccatgac gctggcaact gccgatcata
     8941 atattccgac agtgaaccag aatatgccca tcagggatga actgtcgcgc aggcaggtag
     9001 agaccctcac aaggaactgc aatgagttcg ggataacctg ctatggcctt aatgatccgc
     9061 ataacggaat tgtgcatgtc attggtccgg aactgggcta tacccttccc ggcatgacaa
     9121 tagtgtgcgg cgacagccat acatccacac acggggcttt tggaacggtt gcattcggaa
     9181 tcggcaccag cgaagtggaa atggtactgg ccacacagtg tatcctgcag cccaaaccga
     9241 aaaaaatgag aatacgcgtt gagggcgaac tgggcagggg cattactgca aaggatctta
     9301 tactctatat catatcacag ctgtcaacaa gcggtgccac cggatatttc gtggaatatg
     9361 ccggttctgc cgtcgaaagc ctcagcatgg aaggcaggat gacggtgtgc aacatgagta
     9421 tagagatggg agccagagga ggcctgattg cccctgatga tacaactttc gaatatctca
     9481 ggaagatacc cgcactgagc aaccgtcctg acttctatgc gcttgtcaaa aaatggtcgc
     9541 gcctgagaag cgatccggat gccgtttttg accgtgaact gaccttcgat gcaggaaaaa
     9601 taaaacccat gataacatac ggcacgaatc cgggaatggg tatttccatt gatgaatcaa
     9661 ttcctgccct tgaggaaatc agtgatgaaa caaaggcatc attcatcaaa tcgcttaatt
     9721 atatgggatt tactcccgga atgaaactgg ccggacagcc cgtcgactat gtgttcctgg
     9781 gcagctgcac caacgggagg attgaggatc tcagagcttt tgcaggcttt gtcaggggaa
     9841 ggaaaaaagc ccccggagtt accgtactca ttgttccggg ctcaaagaaa gttgaaaagc
     9901 aggcaattga cgaagggctg gcagatatcc tgaaggatgc cggattcgtg ttgagacagc
     9961 cgggctgctc atcatgcctg gccatgaatg aagataagat accgccggga aaatatgccg
    10021 tgtcaacatc caacagaaat tttgaaggca gacagggacc gggggcgaga acactgcttg
    10081 caagtccgct tactgccgca gccgctgctg taacagggag aataaccgat cccggggaac
    10141 tgatgcagga ttaaccatac acagatacgg gaacctataa aacaaatcag atacctataa
    10201 taataattgc catgccaaaa gagaaatttg atattctgga atcaacttgc atacctctgc
    10261 ctatcgaaaa cattgatacc gatcagatta ttcctgcacg gtttctgagc ctcacctcaa
    10321 gggaaggctt tggcgaatat cttttccgcg actggagatt tgacaaatca ggcaataaaa
    10381 ttgaagactg tgtttttaat aatccacgtt attcgggccg gatactggta accgggaaaa
    10441 atttcggaag cggatcaagc cgtgaacatg ccgtatgggc aatagcagga tacggattcc
    10501 gggcagtggt atcaagcttc tttgcggata tattccggaa caactgcctg aacaacggaa
    10561 tcctgccggt ggtggtaaag gaggattatc tgcagaacct gctgaaaaca atcagggaca
    10621 atccctccac catggcaagg atagaccttg acaaacagct tttcaccgtt ctgcccgacg
    10681 gcgaaggcat aagctttgaa ataaacccgt ataaaaagga atgccttctc aatggcctcg
    10741 atgacattga ctacctgtta agcatacagg atgccatcaa agcatatgaa ttacgaaaaa
    10801 ctcttggaaa atgagaatag aaataatgga caccaccctc agggacgggg aacagaccca
    10861 gggtgtttcc tattcaccct tcgaaaaact tcatctcgca aaattccttc tgaaggatct
    10921 cagaattgac cgcatcgagg ttgcttcagc ccgtgtttca aaaggggaat ttgaagccat
    10981 caggcagata accgaatggg ctgcccggaa tgattttcac aacaaagtgg aagttctcgg
    11041 attcattgat ggcgaaactt cccttaactg gatacgggat gcgggagcca gggtcataaa
    11101 cctcctttgc aagggctcac tgagacatct ggaaaagcag ctcagaaaaa cccctgaaac
    11161 acatgctgcc gacatcaggg atatcatcag aaaaaccgtg gattcgggaa tggaaataaa
    11221 tatctacctg gaagactggt ccaacgggat gatccattcg gaagactatg tattcttcat
    11281 gattgattca atcagggatg aacccgtaaa aagaataatg ctccccgata cgctgggaat
    11341 tctgaatccc gatcagactt attcattctg taaaaaaatc gttgacacct atcccgggat
    11401 acatttcgac tttcatcccc ataacgatta tgacctggca gtggcaaatg tcttttctgc
    11461 cgttaaagcc gggatcaggg gagttcacac caccattaac ggaatgggcg aaagggcggg
    11521 gaatgcgccc ctgtcaagcg ttatcggagt ccttcacgac cagctggaag cggagacagg
    11581 gataaatgaa atggaaataa ccatggcaag ccgtattgtc gaaacattca gcggaatacg
    11641 ggtgccgccg aacaagccgg tcatagggga aaacgtgttt acccagactt cgggtgttca
    11701 tgccgacggt gacagcaagg acaatctcta tttcaacaac cttctgcccg agagattcgg
    11761 aagaaagaga aaatatgccc tgggcaaaca gtcgggcaag gccacgataa gaaagaacct
    11821 ggaggaattc ggactggctc tcagtgccga atcaatcgat aagataaccc agagagtaat
    11881 tgaactgggc gataagaagg aaagtgtgac aactgaggat cttccgttca ttatatccga
    11941 tatcctggga accgaacaga tagtctacag aatattaata aagaattatg ccctttccac
    12001 cagttacgga ctaaaaccct ttgcctccgt aaagatggaa attgacggtc agatccatga
    12061 aggcacatca gccggcgacg gacagtatga cgctttcatg agagccgtaa gaatgatcta
    12121 cgagaaggtt ggcaaggaac tgccccatct gacggactac caggtttcaa taccgccagg
    12181 gggaaagacg gatgcgctcg ttgaaaccat aataagctgg aagcatcacg ataaggaatt
    12241 ccgtaccaaa ggactggatg tggatcagac aggttcggca ataaaggcta ccgaaaaaat
    12301 gctgaacctg atagaaagcc ttaacaacgg aacggaaata aaaacaatgc ccgtctgaat
    12361 ccgcagcaga attattcaat caataaatta tatcaacctc atgaacaaaa attacaatat
    12421 tgccgtcatt cccggtgacg gaacaggtcc tgaagtagta agggaaggta aaaaagtgct
    12481 cctggccgca gcatccagat tcggattcag catcggtttc ggggaatatg acttcggcgg
    12541 ggacagatac ctgagaacag gggaaatatt gccggattcc gctgttgagg agctgaaaaa
    12601 atacgattca atattcctag gagccatcgg tcatcccggt gtcaggccgg gaatccttga
    12661 aaaaggaatt ctcctccgcc tccgcttcga actagaccag tacataaacc ttcgcccggt
    12721 aaagctttac cccggggtgg ataccccgct gaaagacaag ggacccgaac atatcgacta
    12781 tgtggtgata agggaaaaca gcggcgacgt ctataccggt tcgggcggcg tgacaatgaa
    12841 gggaacagca cacgaaattg ccattcagaa catggtgtat tcacgaatgc aggtagacag
    12901 atgccttgaa tatgcctttg aatatacaag gaaaagaaac agaaagaaga ctcttgcgct
    12961 ttgcggaaaa accaatgtcc ttacatatgt tttcgacctg tgggaaaggg catttcatga
    13021 aataggagaa gcaaaatacc ccgatatcaa aagagaatat taccatgttg atgccatcag
    13081 catgtggatg ataaaaaatc cggaatggtt tgatgttgtt gtaacaagca acatgttcgg
    13141 ggatatcatc accgacctcg gggccatcac ccagggagga atgggagtgg cagccggagg
    13201 aaatataaac ccggaaggag tgtcaatgtt tgaaccgatc ggcggatcag caccaaaata
    13261 cacaggactg aatgtgatca atcccattgc agcaatctcc tgcggggcta tgatgctcga
    13321 ccaccttggg gagggaaagg cagcaaatgc cattgagaat gccattatgg acgtcaccgc
    13381 aaacaaactg aaggatatgg gcgccggccg tatgggctac tccaccagcg aagtcggaga
    13441 ccttgtggca gaaaggatct gagtcgtctg aaaatgttta cggcagacca ggtcttcaaa
    13501 ctgcatgatt gctgcaataa tttaattgtt actgcacact ctgcgtttat aataatttga
    13561 tttttttgaa atatttggat tttatcaaat aattattact tttacgctac aattttcaac
    13621 catgtatacc tccaaattgc atcatcacca ttttctttcc tgatctcagg cgaggaatga
    13681 tgatttttgt aatcaggata taattaaccc gtctgagaat accaggcggg ttttattttt
    13741 taacggatat gaacagacta aagattgcaa ttcagaaaag cggacgatta aatgaaaaat
    13801 cccgcagcct gctggcagaa tgcggaataa aatttcccaa cggaacaggg cttcttaaaa
    13861 catcggcaag aaattttccc gtggatattt tgtttctcag ggatgacgac attccccagt
    13921 atgtggaaca gcaggtggct gatatcggaa tcctgggcga aaatgtcgtc tatgagaaaa
    13981 acaggaacgt aagcactctg gagaagcttg gttttgcaaa ctgccggctc agccttgcca
    14041 ttcccggaga ggatagattt acaggtccgg aatatttcaa caacaagaga atcgcaacaa
    14101 gccatccgcg gctcctgtca gaatatcttt cagcgaacag tatttctgcc cgtattgagg
    14161 aaataagcgg cagcgttgaa atagccccgg gaataggcc
//
LOCUS       DAOSPX010000047        14097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4389_length_14097_cov_4.437402, whole genome shotgun sequence.
ACCESSION   DAOSPX010000047 DAOSPX010000000
VERSION     DAOSPX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14097
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4389_length_14097_cov_4.437402"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1721
                     /locus_tag="PLZ75_03935"
     CDS             219..1721
                     /locus_tag="PLZ75_03935"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HOS71582.1"
                     /translation="MIKKICVLIFLCASSYNILYSNSFESEVRKMNKNFNGPLKTNPH
                     NPNYFTDNKGKAVYLTGSHTWANFQEIGIDTTKRFDYDDYLEMMINCHHNFMRLWMFE
                     NSAFASWTPDTVLWHPNIYQRSGQGKAGDGGTRFDLTRFNQYYFDRLRQRIIQAGNRG
                     IYVSVMLFQGWSLDKTGISDPWRAHPWNKNNNINGIGAKDSIADNNSSATIHSLKNPE
                     LLKIQEEYLKKVIETVNDLDNVLYEVSNEGGDHDWHEWVINFVRKYEGKKTKQHVIGY
                     TTPSQQGHLNKNLYESSADWVSPAMEPLGWAYPGTLYMQNYMEDPPANKFGKVNILDT
                     DHLWGHGGNWKWVWKSFTRGYNPIFMDPWQPIEYHGDQRVSWVFVGGVSKDNRDYPDW
                     DLVRTNMGYTRKIAESVNLIDLFPEPDLASTGYCLTDKKNVFIVYLPEGGDVTLNMLS
                     CDVNANYHIEWFFPILNRSMEINKPVTGGGYKNFSLPFSGDGVLILTKTP"
     gene            1985..2827
                     /locus_tag="PLZ75_03940"
     CDS             1985..2827
                     /locus_tag="PLZ75_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS71583.1"
                     /translation="MTLTRRNFIKTTGAGLIAAGAPVLASGSFSHPVPEAKYPFRLAI
                     AGYTFAKFNLDQTLEMMKRVNIGLLGVKDFHLPLDSSQEVINQTLEKMKSFGVEPYGV
                     GPIYMKSEEAIDQAFAYAGRVGTNTLVGVPDVHLLPYVEKKVREYNIRLAIHNHGPDL
                     PIYPGAIDVWKEIRDLDPRMGFCLDIAHSVRLGQDPFRDFLNYKERIFDFHIWDVDKA
                     EKSGWCVESGRGLIDFPRFFRMLKEHNYTGTCSLEYAKDMDDPLAGIAESIGYFRGVL
                     AGLA"
     gene            2853..5123
                     /locus_tag="PLZ75_03945"
     CDS             2853..5123
                     /locus_tag="PLZ75_03945"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOS71584.1"
                     /translation="MKTARLFTLLLLLSAILYHGCNSEKENNREPVDYVNPYIGNISH
                     LLVPTYPTVHLPNSMLRVYPERADYTTLKLRGLPLVVTSHRGRSAFNLSPFQGEAGNL
                     APVISYTYDHEKISPYSYSVVLDEQNIGVRMGVSRQSALYEFTFPDDGKKNIILNSGY
                     GEMKWDGKAVSGYQNLNERTKVFIYLEPDVRPSAAGVLKDGRMDESLSEASGRNAAVV
                     LSFPGSTPALKLRYGISFIDADQAKRNLEREIRDFDLGKIQSEGRRIWNEALGKIRVR
                     GGSEDDRTVFYTSVYRIYERPVNITEDGRYFSAFDGKVHDCEGVPFYTDDWIWDTYRA
                     AHPLRVLTEPELETNIIRSFLRMTEQMENPWMPTFPGITGDSRSMNSNHGVASVIDAY
                     RKGLQDFDLEQAYEACRRAIMEKTLAPWSGKPAGRLDDFYKEHGYIPALAENEKETIP
                     EVHPFEKRQAVAVTLGTAYDQWCLAQIAKELGKTDDYRYFLECSYNYRKLFNAETDFF
                     HPKDSRGNFIMPFDYKFSGGMGARDYYDENNAYTYRWDVQHNIADLIKLMGGREKFVK
                     RLDETFNEPLGRSKYAFYAQLPDQTGNVGQFSMANEPSLHIPYLYNYAGQPWRTQKLI
                     RTLLFEWFRNDLMGVPGDEDGGGTSAFVVFSQLGFYPVTPGSPTYNIGSPVFTESVMD
                     LGNGREFKIVALNCSEENKYIQSARLNGREWNKPWFSHDDIKDGATLELVMGPSPERK
                     WGSDADSAPPSAERME"
     gene            5230..5475
                     /locus_tag="PLZ75_03950"
     CDS             5230..5475
                     /locus_tag="PLZ75_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71585.1"
                     /translation="MKKELTFLRGFILVLIAGMLLGSCSKKEEEATDNLVGKWSVSNV
                     TLVTKVGTRSLSQYLIDELDLTSVEAQQMVQMINAMI"
     gene            complement(5710..5784)
                     /locus_tag="PLZ75_03955"
     tRNA            complement(5710..5784)
                     /locus_tag="PLZ75_03955"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5747..5749),aa:Val,seq:gac)
     gene            complement(5882..7012)
                     /locus_tag="PLZ75_03960"
     CDS             complement(5882..7012)
                     /locus_tag="PLZ75_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71586.1"
                     /translation="MTEKTIFDFQGLEIRVTDRADEGILNILNRAVQGSEGGMRFTFQ
                     NISERIAAYKDRIRFISLYKKNKITATAGACFRISGQCDLRYPSTFVKYLAFHSLFQS
                     DTRSSRRRQERETRTEPFDSFKSRTLELFGKPHLLGLPDVSEKDRHILYAFLESMNEK
                     AKDLVHKAGFEYIRSFLTVAFSRFSPGPDKRVSLIRENEINEMHELLRNYYRNYSLFT
                     ADYAFYGNRYYVLKEGGEIVAGVSAFPTSNKIYNIPGVWGWVMMKVLPFTPYFRRLFS
                     PGIFHFLVFDAIYCKKGKESLLYPLLESACALEGFHTGLMWLDDRSELYDKIRTSGRL
                     GALNRMLNAKPGLVYIKFVNLTEEEQEKFYDAPAYISGFDFS"
     gene            complement(7074..8894)
                     /locus_tag="PLZ75_03965"
     CDS             complement(7074..8894)
                     /locus_tag="PLZ75_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS71587.1"
                     /translation="MKKYYLLRNVTTFLVFFFTVFLLDGQSVVNKRAEWFVDARYGMF
                     IHWGLYSGAEGVWKGESLRNDNNYAEWIQYRNSIGHEEYLGLIDRFKWDEIDPEKWVL
                     LAKDAGMKYVVLTAKHHDGFALWDSKASSYNVARHTSPSRDILRELADACQKHGLRLG
                     LYYSHWIDWDHEYGWNHRMEITGIRPEDYDLYWQNKVIPQMRELLTNYGPVDLIWFDM
                     WIHHSGTVVSREQLIQLKSLIRELQPSCLINSRLGLSLKEDSDVDIHEMGDNQIGSQL
                     QDFPWQSPATVSHSWGFHRRDTEWKSTTSLLRSLINNVSLNGNYLLNIGPRANGEVPY
                     EIAVRLREMGKWMEVNWESVYGCKAFELPEGYHDWGRITCRETAGGTRLYLHLFNYPL
                     DNKLYFSGVTSKPDRVYLLADKQKRPLDYTHKGALATISLPASAPDPYVSVIVLEYDG
                     KPGIHEGLVAKNMDGGYSLTPWNRDNSGIVLKTVNKQRRGTVPAYASITEPTVLKWKI
                     YIDEPGPKNFDISYSFQGETGKGIIRVKADGETLSHSVTPTGKTVAEPNSDWVIDDFR
                     SHRAGIINFNNSGYYDIEVEINPQNREEIKFQWLWICRGG"
     gene            complement(9036..9803)
                     /locus_tag="PLZ75_03970"
     CDS             complement(9036..9803)
                     /locus_tag="PLZ75_03970"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOS71588.1"
                     /translation="MKTRVLFFLTSLFFLTSCAELAKVLQTTGNVPLTEAEVVSGLRE
                     ALGTGAVNAAGLLSKTDGYYGDELVKILLPDEARVIVENISRIPGGERLIEDVILRIN
                     RAAEDAAGEVAPIFLNSIKQMTIRDAFGILNGADNAATNYLRSTTFSELYALYKPKIQ
                     ASTEKKIAGNISTKESWNTLTSKWNSVANSVAGKLAGLNPVNTDLDDFLTDKALSGMF
                     LKVENEELKIRKEVSARVSPLLQKVFGSLDTKTAADK"
     gene            complement(9938..10609)
                     /locus_tag="PLZ75_03975"
     CDS             complement(9938..10609)
                     /locus_tag="PLZ75_03975"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOS71589.1"
                     /translation="MNIYALVVAAGSGERMKSGIPKQFIEIAGKPVLMHAIEKFRIYD
                     SSIRIIVVLPENQTDYWNELRDKYSFTVPHTVAVGGATRFHSVRNGLDLITEPGLVAI
                     HDGVRPLVSIAAIGRCFETAMKLGNAIPVISPSDSLRKTSPDGSEALDRTGIKLVQTP
                     QVFHTSLIKKAFLQDYLPEFTDDAMVLERTGTSINLVEGNRENIKITTPEDLIIAECL
                     IKRTV"
     gene            complement(10723..12375)
                     /locus_tag="PLZ75_03980"
     CDS             complement(10723..12375)
                     /locus_tag="PLZ75_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS71590.1"
                     /translation="MIKERLIGYIEDSIRQNWEIEALSNYREKGYTYRQIAEHILKIH
                     IFFRDTGIGEGDKIALVGRNSANWCMIYLATVTYGAVIVPILPDFKPDDLMNLINHSD
                     SKILFVDDKIFETIDSSKIPGIKASVSVDTLKMIKSADKTVSGVYDSVEDRFSREYPA
                     LKPEDIRFSAVGNDKLAVISYTGGTTGFSKGVMLSHNSLAANVRFAQENMPLKPGDPL
                     VSFLPLAHTYGCAFEFLFPFTYGCHITILSKTPSPQVIIQAFKDIRPRLIISVPLVIE
                     KIFKKQILPVISKPHMKVLLAIPGLNRILHKKIREKLEESFGGRFHEIVIGGAAFNPE
                     AEHFFRKIGLRFTVGYGMTECGPLISYASHDTTKAGASGRPVDTLEVTIDSPDPQKIM
                     GEIILRGENVMLGYYKNEKATKEIIDENGWMHTGDLGLMDRDKNIFIRGRSKTMLLGA
                     SGKNIFPEEIEAVINNMDYIAESLVIEEDDKLIALIFPDSEMMKRDNIQDDQLPEILE
                     KIRKEVNERLPDYMAVNRFRLHPEEFAKTPKRSIKRFLYTKN"
     gene            complement(12444..12851)
                     /locus_tag="PLZ75_03985"
     CDS             complement(12444..12851)
                     /locus_tag="PLZ75_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71591.1"
                     /translation="MILISGDVANFKDILNHILGAVFIVIIFALAYAYLKPHQLHKRR
                     LVSTLLLKISYMVYLTVLLIVVYLAALVKGGLGTVFHGIEFFAFLVVLFAPNIGILAR
                     KLGPFSKKRENYNYFFTVVNVISMLAILVMYFV"
     gene            complement(12854..13825)
                     /locus_tag="PLZ75_03990"
     CDS             complement(12854..13825)
                     /locus_tag="PLZ75_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOS71592.1"
                     /translation="MEKKEVAAGIDIGGTNTIFGLVDREGNILAEDRVITSHYDEIEV
                     FVAALYEKIQLCVSKAGKGIELKGYGVGAPMGNINCGTIEYAAGLPWKGIVPIAAEFR
                     RHTSLPVTVTNDANAAAVGEMIYGGARGMKNFVVITLGTGLGSGFVIDGKLVYGHAGF
                     AGEIGHTAIRPGDSNRDCGCGRKGCLETYVSATGIKRSLLKIMADSIQPSELRKYSFE
                     ELDAKIIHDAALRGDPLALRAFEHTGRMLGFKLADVVAHTNPEAIFLFGGLALAKDLI
                     FEPAKEYMEENLLSIYKGKVKLLASELSTQNAAVLGASSLVWSELEA"
BASE COUNT         3803 a         3230 c         3102 g         3962 t
ORIGIN      
        1 ttggagagta ttatgctttc ccttttttct caatctttta tatcaaaccc cttattgatt
       61 taattaaatc gttgtatctt caaatagcat tatcagaaaa ccctgttttc tagttattct
      121 gtcctttatc cgggatgttt cagaaatata cagactatat accttataaa caaataatta
      181 agattcctgt tagtgtgact gtttaaatcc ttcatatcat gataaaaaaa atctgtgtat
      241 taattttctt atgcgcttcc tcttataata tcttgtattc aaatagcttt gaatcagaag
      301 tgcggaaaat gaacaaaaat ttcaatggtc cattgaaaac aaatcctcac aatccaaatt
      361 attttaccga taataaaggg aaggctgttt atttaaccgg ctctcataca tgggctaatt
      421 ttcaggaaat aggtattgat accactaaaa gatttgatta tgatgattat ttggaaatga
      481 tgataaattg tcatcataat tttatgaggc tgtggatgtt tgagaactct gcctttgcca
      541 gctggacacc ggataccgta ctatggcatc cgaatatata tcaacgttca ggacagggaa
      601 aagctggtga cgggggtacc agatttgatt taaccagatt taatcaatat tattttgacc
      661 gtttacgtca gcgtattatt caggccggga accggggaat ttatgtatca gttatgctgt
      721 ttcagggctg gagtctggat aaaaccggta tctctgatcc atggagagcg catccgtgga
      781 ataaaaacaa taatattaac ggcataggag ccaaagacag tatagccgac aataactctt
      841 cggctacaat tcatagcctg aaaaatcctg aattactgaa aatccaggaa gaatatttaa
      901 agaaagtaat tgaaacagta aatgatcttg acaatgtttt atatgaagtc agtaatgaag
      961 gcggagatca tgattggcac gaatgggtta taaattttgt ccgtaagtat gaaggtaaaa
     1021 aaaccaaaca acatgtcatt ggttatacta caccaagtca gcagggtcat ttaaacaaaa
     1081 atttatatga aagctctgct gactgggtat caccggctat ggaaccgtta ggctgggcct
     1141 atccgggaac actttatatg caaaattaca tggaggatcc gccggccaat aaatttggga
     1201 aagttaatat tttggatacc gatcatttat ggggacacgg tggtaattgg aaatgggttt
     1261 ggaagagttt caccagaggt tataatccaa tctttatgga cccctggcaa cctattgaat
     1321 atcatggcga tcagcgagtt tcatgggtct tcgtgggtgg tgtgagcaaa gataatcgtg
     1381 attatcctga ttgggatctt gtacgtacta atatgggata caccaggaaa attgcagaaa
     1441 gtgttaattt gattgattta tttccggaac ctgatctggc ttcaacaggg tattgtctga
     1501 cagataaaaa gaatgtattt attgtgtatt taccggaagg tggtgatgtc actttaaata
     1561 tgctttcatg tgatgtaaat gcaaattacc atattgagtg gtttttcccg attctcaaca
     1621 gatccatgga aataaacaaa ccagtaaccg ggggcggata caaaaatttc agtttgcctt
     1681 tttctggtga cggagtgctg attttaacca aaactcctta aaataatcat taaaagtacg
     1741 tgaaggttaa ttgcccctta aaagatatta ttatctgaat ttattccgga tttatttttg
     1801 taattcattc aagttataag aaacttgtca aacaggattt tctaaataca cacaactgta
     1861 tgaagtccac gaaaatattg cttcataaca tagcccgtct caattttctt taattaaaca
     1921 attattttat atatttccat taatatatca gacaagaaaa atctaacgaa acagcgctgt
     1981 taacatgaca ctcaccagac gtaacttcat caaaactacc ggagcagggc tgattgcagc
     2041 cggagctccc gtgcttgcat caggttcatt cagccacccc gtccctgagg caaagtatcc
     2101 gttcagactg gccattgccg gttatacctt tgcaaagttc aatctcgacc agacactgga
     2161 aatgatgaaa agagtgaaca ttggcttact cggagtgaag gattttcatc ttcctctcga
     2221 cagttcgcag gaagttatca accagacact ggaaaagatg aaatcgttcg gagtggaacc
     2281 ctacggtgtg ggtcccatct acatgaaaag tgaggaagcc attgatcagg ccttcgccta
     2341 tgccggaaga gtagggacaa atacccttgt tggagttcct gatgtccatc ttcttcccta
     2401 tgtggaaaag aaggtcaggg aatataatat ccgcctggcg attcataatc acggaccgga
     2461 tctgccgatc tatccgggag caattgatgt gtggaaggag atcagggatc ttgatccgag
     2521 gatgggcttc tgccttgata ttgcacattc ggtaagactg ggccaggatc ctttccggga
     2581 tttcctgaat tacaaggaaa ggatttttga ttttcatatc tgggatgtgg acaaagcgga
     2641 aaaatcgggc tggtgtgttg aatccggacg cggattaatt gatttcccgc gctttttcag
     2701 aatgctgaag gaacacaact ataccggtac atgcagcctg gaatatgcga aagacatgga
     2761 tgacccgctg gcaggaatag ctgaatcaat tggttatttc cgcggtgtgc tggccggcct
     2821 ggcctgatgc ggaaacttta aaaaacagat gaatgaaaac tgcaagactt tttactttat
     2881 tattactgct gtctgccata ctttatcacg gctgtaattc tgaaaaagaa aacaacaggg
     2941 aacccgttga ttatgtaaat ccctatatcg ggaacataag tcatctgctg gttcctacct
     3001 atcctaccgt tcacctgccc aacagcatgc tcagggtata tcccgaaagg gcggattata
     3061 caactctgaa actaaggggc ctgccccttg tggtgaccag ccacagggga cgttcagcct
     3121 tcaacctcag tccctttcag ggagaagcag gtaatctcgc tccggttatc agctatacct
     3181 atgatcacga gaaaataagt ccgtattcat attcagttgt tcttgatgag cagaatatcg
     3241 gagtaaggat gggtgtttca cggcagtcgg cactttatga gtttactttc ccggatgacg
     3301 ggaaaaagaa tatcatcctg aattcaggct acggggaaat gaaatgggac ggaaaggctg
     3361 tttcaggata ccagaatctt aatgagcgca cgaaagtttt catttacctg gaacctgatg
     3421 tcagaccctc agcggcgggt gttctgaaag acggacggat ggatgaatcc ctctcagagg
     3481 cttccggcag gaatgcagcc gttgtactca gttttcccgg cagtacccct gcacttaaac
     3541 tgcggtatgg catctcgttt atagatgcag accaggcgaa aagaaacctt gaacgcgaga
     3601 taagggattt tgacctgggg aaaatacaat ccgaaggtcg cagaatatgg aatgaggctc
     3661 tcggaaaaat cagggtcagg ggaggatcgg aagatgacag aacagtattt tatacatctg
     3721 tttaccgtat ctatgagcgt ccggtgaaca taacagaaga cggaaggtat ttcagtgcct
     3781 ttgacgggaa agttcatgat tgcgaaggtg ttcccttcta tacggacgac tggatatggg
     3841 atacctacag ggcagctcat cccctgagag tcctgacaga accggaactc gaaaccaata
     3901 ttatcagatc atttctgaga atgaccgaac agatggaaaa tccctggatg ccgacatttc
     3961 ccgggataac aggcgacagc aggagcatga attccaatca tggtgtggca agcgtcattg
     4021 acgcatacag aaaaggtttg caggattttg atctggaaca ggcctatgaa gcctgcagaa
     4081 gggccataat ggaaaaaacg ctggcgccct ggtcgggaaa acctgccggc agactcgatg
     4141 atttctacaa ggaacatggt tacatccccg cacttgctga gaatgagaag gaaacaattc
     4201 cggaagttca tcctttcgag aaaaggcagg cagtggccgt cacactcggg acagcctacg
     4261 accagtggtg tctggcacag attgcaaaag agcttggaaa aacagatgac taccggtatt
     4321 tcctggagtg ctcctataat taccgtaagc ttttcaatgc agaaactgac tttttccatc
     4381 cgaaagacag caggggaaat ttcatcatgc cctttgacta taagttttcc gggggcatgg
     4441 gtgcccgcga ctactatgat gaaaacaatg catacacata caggtgggat gtccagcata
     4501 atattgccga cctgataaag ctgatggggg gcagggagaa gtttgtaaaa aggcttgatg
     4561 agacattcaa tgaacctctt ggcagaagca agtatgcatt ctatgcacag ctgcccgacc
     4621 agaccggaaa cgtcggccag ttttcaatgg caaatgaacc atctctccat ataccctacc
     4681 tgtataatta tgcaggccag ccatggcgta cgcaaaaact gatcagaaca ctgctttttg
     4741 aatggttccg caacgacctg atgggagttc ccggtgatga ggacgggggc gggacatctg
     4801 cattcgtggt attctcccag ctgggattct atccggttac cccgggatct cccacgtata
     4861 acattggcag tcctgtattc actgaatcag tgatggatct tggaaacgga agggaattta
     4921 agatagtggc acttaactgt tctgaagaaa acaaatatat acagtcagca aggcttaacg
     4981 gcagggagtg gaacaagccc tggttcagtc atgatgacat caaggatggt gcaacactcg
     5041 aacttgtgat gggtccgtct cctgaacgca aatggggaag tgatgcggat tcagcacctc
     5101 cttcggctga aagaatggaa taaacccgcc gggatataaa tcctgccacg aatatatttt
     5161 ccgtatgtct tttctgaaca aaacagattg gaatctgttt agatttatta ttaattaaat
     5221 ctgtagacca tgaaaaaaga attgacattt ttacgggggt ttattctggt attgattgcc
     5281 ggtatgcttt tgggttcatg ctcaaaaaaa gaagaagaag ctaccgataa tctggtaggt
     5341 aaatggagtg tcagcaatgt aacattagtc accaaggtcg ggaccagatc actttcacaa
     5401 tatttgatag atgagctgga tttaacttct gttgaagccc agcagatggt tcagatgatc
     5461 aatgctatga tctagccggt cctcagattt ttgatgtcct tgaactaagt tccggcaagc
     5521 ttgtactcaa aatcacccag caggaggatg tggatctcga tggtgataat aagggtgaaa
     5581 ccttaaccgt tacaatcgag atgaccctga gtaaatagca gatagattta attaagcggg
     5641 aaatattctg tattctgaag ggaacttttt cagagagtca tgatggcatg actccctgat
     5701 tttcctttgt ggggcgtact ggattcgaac cagtgacctc ttgcctgtca agcaagcgtt
     5761 ctgaaccaac tgaactaacg tcccgtcatc atgccggcat cagacttccg ctatcagtaa
     5821 taaatgtccc ggatttccgg gttagcgggg atctcctgcc agctgcaaaa atagtaaatg
     5881 cttatgaaaa atcaaatccg gaaatatagg caggggcatc atagaatttt tcctgctctt
     5941 cctcagtcag attcacaaat tttatataaa caagtcccgg cttggcattg agcatccggt
     6001 tcagagcccc cagcctgccc gatgtacgta tcttgtcata taactcactc cggtcgtcaa
     6061 gccacatcag cccggtgtga aacccttcca gagcacaggc cgattcaaga aggggataaa
     6121 ggagagattc cttccctttt ttgcagtaaa tggcgtcaaa caccaggaag tgaaaaattc
     6181 ccggactgaa aaggcggcgg aaatacggtg tgaaaggaag cactttcatc atcacccagc
     6241 cccatacacc gggaatattg tatatcttgt tggaggttgg aaaggcactg actccggcaa
     6301 ctatctcccc gccttccttc agaacgtaat acctgttgcc gtaaaaggca taatccgcag
     6361 tgaaaaggga ataattacgg taataattcc tgagaagctc atgcatttca tttatttcat
     6421 tctccctgat cagtgatacc cgcttgtcgg gtcccggact gaacctgctg aaggcaactg
     6481 tgaggaatga tcttatatac tcaaatccgg ccttgtgaac aagatccttt gccttctcat
     6541 tcatactttc aagaaaagca tacagaatgt gcctgtcctt ttcggaaaca tcgggcagtc
     6601 ccagcagatg gggcttcccg aaaagttcaa gtgtcctgga cttgaaggaa tcgaatggct
     6661 ctgttcttgt ctccctttcc tgtcttctcc tgctgctcct ggtatccgac tggaacaatg
     6721 aatgaaaagc cagatacttg acaaaggtgg aagggtatct gagatcgcac tggcctgaaa
     6781 tacggaaaca ggctccggcc gttgccgtga ttttattttt tttatagagg gagataaacc
     6841 tgatccggtc cttatatgcg gctatcctct cggaaatgtt ctggaaggta aacctcatac
     6901 ctccttccga accctgaaca gcacggttga gaatgttaag aataccttca tcagccctgt
     6961 ctgtcaccct tatttcaagt ccttgaaaat caaaaattgt tttttcagtc ataatctgcc
     7021 tgctgatcct tattaattca caaaaccgga ttcagaaccc ctgtaatacc ggtttatcct
     7081 ccccggcaaa tccagagcca ctgaaactta atttcttccc tgttttgagg atttatttcc
     7141 acctctatgt catagtaacc ggaattatta aagtttatta tgcctgcgcg gtgcgatctg
     7201 aaatcatcta ttacccagtc agaattgggt tcagccactg tttttccggt tggtgtcaca
     7261 ctgtgtgata atgtttcccc gtcagccttt accctgatta tacctttgcc tgtttctccc
     7321 tggaaactgt atgaaatgtc aaagtttttc ggtcccggtt cgtcaatata tattttccat
     7381 ttaagtacag tgggttccgt aattgaagca taggccggaa cggttcccct gcgctgttta
     7441 ttcacggtct tcaggacaat gccggaatta tctctgttcc acggtgtgag ggaatatccc
     7501 ccgtccatgt tttttgccac caggccttca tgaatcccgg gctttccgtc atattcaagc
     7561 acaataacgg atacataggg atcaggtgcg gatgccggca gggaaatcgt tgcaagagcc
     7621 cccttatggg tatagtcaag tggccttttc tgtttatctg caagaaggta aaccctgtcc
     7681 ggctttgatg tcacgccact gaaatacaat ttattgtcaa gaggataatt gaacaggtgg
     7741 agatacagcc ttgtgccgcc agccgtttcc ctgcaggtaa ttctgcccca gtcgtggtag
     7801 ccttccggga gttcaaatgc cttgcagcca taaaccgatt cccagtttac ttccatccac
     7861 ttccccattt cacgcaaacg cactgcaatc tcataaggaa cctccccgtt ggccctcgga
     7921 ccaatattaa gcagataatt cccgttgagg cttacgttat tgatcaggga ccggagcaat
     7981 gatgttgtgg atttccattc ggtatcgcgc ctgtgaaaac cccaggaatg agaaactgtg
     8041 gcaggggatt gccagggaaa atcctgcagc tggcttccga tctgattatc ccccatttca
     8101 tgtatatcaa catcactgtc ttcctttaaa gaaagaccca gacgggaatt tatcagacac
     8161 gagggctgaa gttcgcgaat aaggctttta agctgtatca gctgttccct gctgaccacg
     8221 gtcccggaat gatgtatcca catgtcgaac cagataaggt cgacgggacc gtagtttgtc
     8281 agcagttccc gcatctgggg tattaccttg ttctgccagt aaagatcata gtcttcgggc
     8341 ctgatgcctg ttatttccat cctgtggttc cagccgtatt catggtccca gtcgatccag
     8401 tgggaataat aaaggccgag ccgcaagcca tgtttctgac aggcatcagc aagttccctc
     8461 agaatatccc gggaaggact tgtatgccgg gcaacgttat aagagcttgc cttactgtcc
     8521 cacagggcaa atccgtcgtg atgcttggct gtgagaacca catatttcat acctgcatcc
     8581 ttcgcaagaa gcacccattt ctcaggatca atctcatccc atttaaagcg gtcaataagg
     8641 ccaaggtatt cttcatgccc tatactgttc ctgtactgta tccactcagc atagttattg
     8701 tcatttctca ggctttcgcc tttccagact ccctcggctc cgctgtaaag gccccagtga
     8761 ataaacatgc cgtagcgggc atcaacaaac cattccgccc ttttgtttac aactgactgg
     8821 ccatcaagca ggaaaacggt aaagaaaaaa accagaaacg ttgtgacatt ccgcagtaag
     8881 taatattttt tcataataac cggcattggt ttatctgaat atcctttaac tatccgggat
     8941 ccggtgattc cgatgaatcc gggatcgggt taatccggag aatccggcat ccggcaaaac
     9001 cggtaaaaca atacgctatg caactcaatc ccctgtcatt tatcagccgc cgtttttgta
     9061 tcgaggcttc cgaacacttt ctgaagcagg ggactgactc tggcgcttac ttcttttctt
     9121 attttaagtt cctcattttc aactttcaga aacattcctg aaagagcttt atctgtaaga
     9181 aaatcgtcca gatcggtatt tacaggattc aggccggcaa gttttccggc aactgaattt
     9241 gcaacggaat tccattttga agtaagggta ttccaggatt cctttgttga tatgtttcct
     9301 gctatcttct tttccgtgga tgcctgtatc tttggcttat agagagcata gagctcactg
     9361 aaggtggtgc ttctcagata attcgtcgcg gcattgtctg ctccgttcag aataccgaag
     9421 gcatccctga tagtcatttg ttttatactg ttcaaaaata tgggagccac ttccccggct
     9481 gcatcctcgg cagcccggtt gattctcagt atgacatcct ctatcagcct ttccccgccg
     9541 ggaatccttg aaatattctc aacaatcacc cttgcctcat cgggcagaag gatctttacc
     9601 agctcatcgc cgtaatatcc gtccgttttt gaaagcagac ccgcagcatt aacggcccct
     9661 gtgcccagag cctccctgag accgctgaca acctcggctt cagtaagggg gacattcccg
     9721 gttgtctgaa ggacttttgc cagttcagca catgatgtca gaaaaaataa agatgtaaga
     9781 aaaaataaaa cccttgtttt catttgcagc taaattgaaa ttccctgcaa tatacaaaat
     9841 atcagaaaaa cagagattat ttcatcacac agtctccagc gtcaggatac cccgttgaac
     9901 aggtcttccc gataacggaa ataccggcag gccatgttca gacagtcctt tttatcaggc
     9961 attcagcaat gatcaggtct tcaggcgtgg ttatcttgat attttccctg ttaccctcaa
    10021 caagatttat acttgtaccc gttctttcca gcaccattgc atcatctgtg aattccggca
    10081 gataatcctg aagaaaagcc ttttttatca ggctggtatg aaacacctgg ggtgtctgaa
    10141 caagctttat cccggttctg tccagagctt cactcccgtc agggcttgtc ttcctcagtg
    10201 aatctgaagg cgatattacg ggtatggcat tcccgagttt catggcagtc tcaaaacatc
    10261 tgccaatggc agcgatactg acaaggggcc tcacaccgtc gtgaatggca acgagtccgg
    10321 gttcagtgat cagatccagt ccgtttctca ccgagtgaaa gcgggttgca ccgcccactg
    10381 caaccgtatg cggaacggtg aatgaatatt tatccctgag ttcattccag taatctgtct
    10441 gattttccgg cagtactaca attatccgaa tggagctgtc atatatccgg aacttttcaa
    10501 ttgcatgcat cagtacaggc tttccggcaa tctcaatgaa ctgcttcggt atgccgctct
    10561 tcatcctttc gccggatccc gctgcaacaa ctagggcata aatattcatg ggaagaaaag
    10621 ataatccaat aattcctcct gttctttctt tatacttcct gaaggctcag ggtaacctgt
    10681 taatcattac gtctcccgtc cctccaggga ttctgtcccg gttcagtttt tcgtatacag
    10741 gaatctcttg atgctcctct ttggtgtttt ggcaaattcc tcgggatgga gtctgaatct
    10801 gtttacagcc atataatcag gaagccgttc attaacttct tttctgattt tttcaaggat
    10861 ctcaggcagc tgatcatcct ggatattatc tcttttcatc atttcagaat ccgggaaaat
    10921 aagtgcgatc agcttgtcat cctcctctat cacaagtgat tcagctatat agtccatatt
    10981 attgataaca gcctcgatct cttcggggaa aatattcttt ccggatgctc ccaggagcat
    11041 cgttttactt cttcccctga taaaaatatt cttgtccctg tccatcagtc cgaggtctcc
    11101 ggtatgcatc cagccgttct catctattat ttcctttgta gccttctcat tcttgtaata
    11161 accaagcatg acattttcac cgcggagaat tatttcgccc attattttct gcggatcggg
    11221 tgaatctatt gtcacctcga gagtatcaac cggtcttccc gatgctcccg ctttcgtggt
    11281 gtcgtgtgaa gcatagctga tcagggggcc gcattcggtc atgccgtatc ccaccgtgaa
    11341 cctcagtccg atcttcctga agaaatgttc cgcttcggga ttgaaggctg cacctccgat
    11401 gacaatttca tggaaccttc cgccgaatga ttcctcaagc ttttccctta ttttcttatg
    11461 aagtatcctg tttaatccgg gtattgccag aaggaccttc atatgcggtt tgctgataac
    11521 agggagaatt tgtttcttga atatcttctc aatgaccagc ggtaccgaaa ttatcagtct
    11581 gggccgtata tccttgaatg cctgaatgat tacctgtggc gagggtgttt ttgacagtat
    11641 tgtaatgtgg catccgtagg taaaggggaa caggaattca aaggcacagc cgtatgtatg
    11701 tgccagggga aggaatgaga ccagcggatc gccgggtttc agcggcatgt tttcctgggc
    11761 aaacctgaca tttgctgcaa ggctgttatg ggacagcatg acaccctttg aaaaacctgt
    11821 tgttcctcct gtatagctga tgacggcaag cttatcattg ccgacagcgg aaaacctgat
    11881 atcctccggt ttaagagcgg gatattccct gctgaacctg tcttcaaccg aatcatatac
    11941 cccgcttacg gttttatcgg ctgatttgat cattttaagg gtatcgaccg atacggaagc
    12001 ttttatcccg gggatttttg aagaatcgat tgtctcaaaa atcttgtcat ccacaaacag
    12061 tatctttgag tctgaatgat ttatgagatt catcagatca tccggcttga agtcgggtag
    12121 tattggtaca atcacggcgc cataggtgac ggttgcaaga tagatcatac accagttggc
    12181 ggaatttctc ccgacaagcg ctatcttgtc accttcccct atgcccgtat ccctgaaaaa
    12241 tatatggatt ttaaggatat gctccgctat ctgccggtag gtgtatcctt tttccctata
    12301 gtttgacaga gcttcaattt cccagttctg cctgatgcta tcttcaatgt agccaattag
    12361 acgttccttt atcatgtttt cagattttag ttaactattt tgacattatc ccaatatagg
    12421 tggaaaaaat gttaaatgcc agtttataca aaatacatta caagaattgc cagcatggat
    12481 attacattca cgaccgtgaa gaaataattg taattctcgc gttttttgct gaatggtccc
    12541 agcttccggg ccagtatccc gatgttcgga gcaaacagca cgaccaggaa agcaaaaaat
    12601 tctattccgt ggaaaactgt tccgagtcct ccctttacca gtgctgccag atacacaact
    12661 atcagcagga cggtaagata caccatatag ctgattttaa ggagaagggt tgaaaccagc
    12721 ctgcgtttat gaagctggtg gggtttcaga taagcatatg ccagtgcaaa aattatcacg
    12781 ataaagactg caccaagaat atgattcagt atatccttaa aattagccac gtcacccgag
    12841 attagtatca ttactatgct tcaagttccg accatacaag tgaacttgca ccaagaacgg
    12901 cggcattctg tgtgctgagc tcagaggcca gcagttttac cttccccttg taaatgctca
    12961 gcagattttc ttccatgtat tcttttgccg gttcaaatat caggtccttc gccagggcca
    13021 gtcctccgaa caggaagatt gcttccggat ttgtgtgtgc cacaacgtcg gccagtttga
    13081 aaccaagcat tcttcctgta tgctcgaatg ccctgagggc aagcggatcg ccccgcaatg
    13141 ccgcatcatg gattatcttt gcatccagct cttcaaagct gtatttcctc agttcactgg
    13201 gctgaatact gtcagccatg atcttgagca gggatctttt tattccggtt gccgatacgt
    13261 atgtctcgag gcagcctttt ctgccgcatc cgcagtcgcg gtttgaatca ccgggccgga
    13321 ttgccgtatg gcctatttca ccggcaaaac cggcatgacc ataaacgagc tttccgtcaa
    13381 ttacaaatcc gcttcccagt ccggttccca gggtaataac aacaaaattc ttcattcccc
    13441 tggctccgcc gtaaatcatt tcccctacag cagcagcatt tgcatcattg gtaactgtta
    13501 ccggcagact ggtatgtcgg cggaattcgg cggcaatcgg tactatgccc ttccagggca
    13561 atccggcagc atattcaatg gtgccgcaat tgatgttgcc catgggagca cccacgccat
    13621 aaccctttaa ctcgattcct ttaccggctt tgctgacaca aagctggatc ttctcataca
    13681 aggctgctac aaacacctct atctcatcat aatgagaggt tataaccctg tcttccgcga
    13741 gaatatttcc ctcccggtca accagtccga atatggtatt ggttcccccg atgtcaatac
    13801 ctgccgctac ttctttcttt tccattcaag taagataaaa acctgtttat attccggatc
    13861 attccgcccg gatacttgtc gcagcaagtg ctgtccggca cggatgaccc cggtaatgac
    13921 tgctgccccg agtctattca ggctggtata tattacctac cttcctggct ctttgtggtt
    13981 tgtccaggtt caggcccaga gccacaccgg cttcaacaag tatgttccag ccggctgcca
    14041 gctgcaggta ttccgaatac tcacctccgt caggtattat gactgtcggg aagattg
//
LOCUS       DAOSPX010000048        14007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4430_length_14007_cov_4.078555, whole genome shotgun sequence.
ACCESSION   DAOSPX010000048 DAOSPX010000000
VERSION     DAOSPX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14007
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4430_length_14007_cov_4.078555"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLZ75_03995"
     CDS             <1..620
                     /locus_tag="PLZ75_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS71593.1"
                     /translation="SVRQIRDLIKRKKPFVLFDRYFQDVNCNYIILDNFKATYTATRH
                     LIAKGHRKIGLVAYKTSLIHMKERIRGYREALKEAGLEHSKYLAEIKFSHSFRDFENS
                     FRSFINRHSDIEALLCANNGLSISSLYYASQNCIRIPEGIDFIGFDGGEPFDLYSTPL
                     SYVRQPIEEMGREVVKLLTELMNDSSKVSHIMHDPELVIREGRDC"
     gene            785..2857
                     /locus_tag="PLZ75_04000"
     CDS             785..2857
                     /locus_tag="PLZ75_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOS71594.1"
                     /translation="MKNSYLRILPAEIIIPVIFLLIFSGCSRSPEIIWDKWGVPHIYA
                     NDPAGMYYAFGWAQMRSHGDLILELYARSRGRVSEYFGNDYLESDKKILTFGIPELAE
                     SGVSRLAPEYRRYSEAFVKGMNDYIDHNRDSIDSKLLQVYPVSAADILAHTYRVIWLE
                     FLGGEDIYATARMMNPGSNAVALAPSRTTSGKAMLVINPHLPWGDFFTWFEAHLNCPG
                     FSSYGIALAGMPVLTLAFSKYLGWAHTVNPLDASDRYELKLQDGGYILDGQIKQFAQK
                     NYIIRVRQDDGSYDEIPFSVKYSVHGPVAESDDGRAMAIRIAGLEDFGIFEQYHRMAA
                     AKNFTGFESALRLMQSPMFNVVYADRDGNIFYLFNGNIPVRQEGDFNFWRGTIDGSSS
                     KYLWDKYHDYDDLPRLFNPETGFVQNCNEPPWFCTYPPVLKQTDYPPYFSSSWMTLRP
                     QRAINLIRGHEKISFEQLIDIKLNTGMEAADRFLAGLIEASSSSTDPIVLEAVRVLRE
                     WNGKTDTDSRGAVLFAAWMDLVTPDMYIRKWDPEDPADTPSGLKDPEKAAVLLGKAAS
                     RVKDSYGSLDVPWGEIYRFRRGNIDYPANGGPDHLGIFRTMYFQKDVDNKFRAFHGDT
                     FHAVVEFGDNPRAMVLQAYGNSSRKGDRHNGDQIQYLSEKKLRPALLSRKDLRENTEL
                     TEKFNKYK"
     gene            2947..6024
                     /locus_tag="PLZ75_04005"
     CDS             2947..6024
                     /locus_tag="PLZ75_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS71595.1"
                     /translation="MTAAFNPLQGQKAGEQQKRITGVVRDESGQALSGAAVLVEGTTT
                     GTYTDAEGRYTIMVSDDSQVLVFSFMGYMTQKFPVKGRTVIDADMNPDTQTLDDVVVV
                     GYGTQKKVNVSGAVDVVTAKAIEKRAVANLSQSLQGISPSLNISVGNSGGEMGARMEM
                     NIRGVGSITGGSPYILVDGLEQDLNNLNPDDIESISVLKDAASSAIYGARAAFGVVLV
                     TTKKGRNDGISINYSNNYSFSAPTIVPHSVDSKRFATYLNVASLNDGNPPLFQNIILD
                     FIDQYLAGEIDFWTAPYPLAPQYWLLNQGAWANTDWYDINYKKWVPNTLHNLSISGGD
                     QKTQFYVSGSMFDQSGLLNFGTDNYGRYTLNTKINTRILDWLRFNFTSKFSRINIERP
                     SYDRNLLYDRLARQWPTNAPYFPNGTPNYEAVQVWLEQGGMYREKNNELSIIPGLEIE
                     PLKGWIIYSNLRWKMNNWNMSDHEAKVKAWLTDMSMTYLRPENNYSTNNYAGYYMSPN
                     IYSSYSKAFGKHNFTVMAGFEQELFNYESTFAKRYDLISDEVPSLGTATGRQEVAGTQ
                     GHSATRSFFGRLNYGFNEKYLLEFSFRYDGSSKFPPGYRWGTFPSGSMAYLISREDFW
                     KPLSVVVNQFKIRASFGSLGNQNVDNYLYIERLPVSTNLAYIMGNERPNYVGMAAPIS
                     PGLTWEKVNTSNAGIDAGFLKNRLNLSMDYFIRNTHDMLGPAESLPAVLGTAVPRSNN
                     AALRTRGFELSLDWRDHFAGFNYGAKFVLSDAVSTVTRYFNPEKLLSAPYYEGMKLGE
                     IWGFTTVGLFESDEQAQSVDQSYFSADVLRAGDVHYKDMNNDGRIDIGKNTVADPGDK
                     SIIGNSTPRFAYSLTLNADWKGIDMNIIWQGIAKRDLWLSSPIFWGAGGIYWLTAYEG
                     HMDYWTPENTDAYWPRPYIDHGSKNKQVQTRYLQTGAFLRLKSLQLGYTIPQKLTKKL
                     YISNLRIYTSGENLLTFTKLFEAFDPEATEGERAHGYIYPLQKVLSGGVSITF"
     gene            6077..7828
                     /locus_tag="PLZ75_04010"
     CDS             6077..7828
                     /locus_tag="PLZ75_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS71596.1"
                     /translation="MKKRFFSLFILAGLLVSCSEDYLNRTPLDEISPGDFFRTANDLR
                     LYANRFYPLLPSHSGHGGGTFWTDDNSDNLVPGTEDKRLAGTRTVPSSGGGWNWEHIR
                     QVNYFLDHCFLDPDEADLKRTYIAEVRLFKAMIYFDMLRTFGDVPWFTRALNPDSPEL
                     YSGRLSREIVADSIIALTDYAIENLKPAGQSEEFRLNREVAILFKARVCLYEGTWEKY
                     HQGTPFGVQGSDGTRFLQMAEEAAALLMQSGTLNLYKGPEGKEYSSLFNKLDYSGIPE
                     VLLWEKYDIGLGIYHWCSQYLPFGAGNTGVSKSLMDAYLCTDGKPISVSPLFRGYDSI
                     QAESADRDPRLAQSMLLPGDDVTINSPGGSLDKKWWAPALDQSQIFRATTGYCMNKGA
                     NLDYSQQQSSGGIMGSIIFRYTEALLIYAEARAELGIITQDDIDKTVNKIRDRVGMIH
                     LDINNITIDPAWDFPELSPLINEIRRERRVELAFEAQRWDDLARWRAHRLLAGKRPRG
                     IKYIGSNLEGTYKDYLGKPTIVIGQNLFVDSEGRVDPYQVKFPNGLGFNPERDYLSPI
                     PSDELTLNNKLIQNPGW"
     gene            7873..9483
                     /locus_tag="PLZ75_04015"
     CDS             7873..9483
                     /locus_tag="PLZ75_04015"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS71597.1"
                     /translation="MKHTVTLEKLISEMTSRESLSYYPLPDYKMVQASSYNRKSVSPD
                     SAGWFENNDMSHFIRVEENRGRREFVMLDTEGPGALVRWWMTFFKARDGVIRIYLDNS
                     EEPVIQGAPGELLSGTLLAPWPFSASVQEGAPLGEEGRDYDHNLYFPVPFSRHCKVTY
                     ECDSLRILYDYEGLSVPEGYYWPDVFYNINYRLYDKSVKVQSFTLKELEKCRKKLETA
                     GSYLLSEASLPGNKISAKERIMPGDSLVLSDCSGPSAIGFLQAEISSSGLNQSLRSVV
                     LSIRTDGISSVRVPVGDFFGSGYTVQTHRTFFNSRDSAGLMKSFWIMPYRESCSIAFI
                     NYGRDTVEISALAGTIPYRWNDRSMYFGASWHEYHRLRTRDETGSSFDLTFVKLEGRG
                     VYTGDQLVLYNNTYHWWGEGDEKTFVDNESFPSIFGTGSEDYYGYSFGRRESFSHPFI
                     SQPVGTGNMSKGVTINMRHRTLDAIPFRTAIDSRIEMWHWADIKMNIALTSYYYFFAP
                     LTGNIEPDIVSVQNKVAVTGSDFDEADN"
     gene            9651..11711
                     /locus_tag="PLZ75_04020"
     CDS             9651..11711
                     /locus_tag="PLZ75_04020"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS71598.1"
                     /translation="MAGFIFFSLSSCGRKSDIVTQGSLFDEMISLSRLSSARGTNYRA
                     VQFSSYDRRSVSPGDSTWFANEDGFGGEPIPGFEKVLIAPDSSGRGTYLICDVSGPGV
                     IQRFWTAGISGTVRVFLDSPDELLYEGSAEDFFRKPAETLTGDNDLAKHTHSFYQFDA
                     SYFPVPFSKRFRMEWTGNTSDIHFYHVGLRLYNKGQNVESFSRENFIKYRSAMIRTDS
                     LLRNPDNRPAGKRYSSVNETITLESGEKALLMNLEGPSVITRFKVKINAVNPENALRQ
                     AVLRIWFDETENAQVNSPLGDFFAAAPGINPYESLPFSVLTDSTMMCRFEMPFRENAK
                     IAVENYSSGKIEINAEAIVAPFRWKEGESMHFHAGWSMDHGLTTSELDSRASDIPYLI
                     AGGRGRIAGAAALIHNPSSVPTSWGNWWGEGDEKIFIDRDTFPSFFGTGSEDYFNYSW
                     SSSEIFSYPYCGQPRNDGPGNRGYVSNYRWHIADDIPFGEKVKFYMELKHHGRVEGFS
                     YGRIIYFYLLPGDNNLPAMFSENDLKEIRYDPWLPEAFKGSAGWSFVQAEELVSGTED
                     ICIEKDNFWAGSGVLKWKPAHHQDILKLNIHSSGEVRETRIGLTLTHDPQSGRLVFRL
                     NGKPVRFSGSDTLDISTRSGRLLENHFSSPLSLRKGLNELEISMPGADGGKIAKIDFV
                     WLRE"
     gene            11759..>14007
                     /locus_tag="PLZ75_04025"
     CDS             11759..>14007
                     /locus_tag="PLZ75_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOS71599.1"
                     /translation="MKKTGIVRIILLTIIILFQVLPSPCQDREREFNGWYYGRMLDRI
                     SFPVGGLGAGMFCMDGNGSVSHLSVRHHPDLFNEPVCFAAISVKDCENGAKVLEYQVP
                     EWKYYGRPGAGLGFPGKLYGLPRFEKGRFLARFPFAILELEDEDIPLSVELKGWSPFI
                     PGDEDNSSLPAGVFEYSFRNNTDRTLEAVFSWNSRNILTEWAGPVRGIKGGFVMSENS
                     SDENTVRNTGFAVFTDDPSAIVDHCWFRGQWFDPYIILWKNIETQTIPSNPESREPVG
                     GASIYIPFMLDPGEEKTVKLNFCWYLPESGLSIGNIPGTDEKLPPYEVWYSSKFRSLG
                     EVIDYWNTNSERLRKETELFTNALYSSTLPSEVMEAVTANLSILKSPTLLRQADGRLW
                     AWEGCADSEGSCHGSCTHVWNYAQAIAHLFPGLERTLRETEFLVSQDAEGHQNFRVNL
                     PVSEPPHGFHAAADGQLGGIMKVYREWRISGNTEWMRNLYPKVRQSLDYCIKTWDPDH
                     KGYLEEPHHNTYDIEFWGANGMTTSFYLGALNALIEMSKALKAPYKDYEAILKKGIYY
                     ADTELFDGEYYIQKIKWEGLHSPDPVEAARKSFQTNYSPEALLILKEEGPKYQYGSGC
                     LSDGVLGMWLSQVCGLRQVMDNSKITSHLASVHKYNLRNNLTDHANPQRPAYAAASEG
                     GLLLCSWPKGGRLSLPFIYSNEVWTGIEYQAASHLFFQGEVQKGLDIVRTARLRYSGN
                     IRNPFNEIE"
BASE COUNT         3879 a         2978 c         3498 g         3652 t
ORIGIN      
        1 gttccgtcag gcagatcaga gacctgataa agaggaagaa acctttcgta ttgtttgaca
       61 ggtattttca ggatgtaaac tgcaattata ttattcttga caatttcaag gcgacctata
      121 cggcaacccg gcacctgatt gcaaaaggtc acaggaaaat agggcttgtc gcctacaaaa
      181 catctctgat acatatgaaa gagaggatca ggggatacag ggaagctctg aaggaagccg
      241 ggctggagca ctcgaaatac cttgccgaga taaaattttc ccattcgttc agggattttg
      301 agaacagttt caggtctttt attaacaggc acagtgatat cgaggccctg ttatgcgcca
      361 ataatggttt atcgatttcc agtctctact acgccagtca gaattgtatc aggattccgg
      421 aaggtattga ttttatcggc ttcgacgggg gagagccctt tgatctctat tcaaccccgc
      481 tttcctatgt gagacagccc attgaagaaa tgggcaggga ggttgtgaaa ctgctgacag
      541 aactgatgaa cgactcatcc aaggtgtctc atattatgca cgatcccgaa ctggttatca
      601 gagaaggccg ggattgttga tttctttgat tcctgcttaa acgttttgtc atttttaaag
      661 taacaatgaa aatgaaatga attgaactga actgaactgc acaggacagg acaacgaacg
      721 ggatccggtt ctggttccgg ttcgggagca ggcctggcaa taacctctat aacaattaag
      781 atctatgaaa aacagctatc ttagaattct tccggcagag attataatcc ctgttatttt
      841 ccttttgatt ttttcgggat gcagccgcag tcctgaaatc atatgggata agtggggggt
      901 accccatata tacgcaaatg acccggccgg gatgtattat gcattcggat gggcacagat
      961 gagatctcac ggggatctta tcctggagct gtatgcccgg tcgagaggca gagtttccga
     1021 atatttcgga aacgattacc tggagagcga caaaaagata ctgactttcg ggattcctga
     1081 gcttgctgag agcggagtca gcagactggc tccggagtac cgcagatatt ccgaggcatt
     1141 cgttaaaggc atgaatgatt acattgatca caaccgggat tcaattgaca gtaaactcct
     1201 gcaggtttat ccggtgagcg ccgcagatat cctggcacat acttacaggg ttatttggct
     1261 tgaattcctt gggggagaag acatatatgc cactgcccgt atgatgaatc ccggttcaaa
     1321 tgcggttgca cttgccccgt ccaggacaac atcaggcaaa gcaatgcttg tgataaatcc
     1381 tcatctcccc tggggtgatt tctttacctg gttcgaggca catcttaatt gccccggttt
     1441 cagcagctac ggcattgccc tggccggtat gccggttctt acactggctt tcagcaaata
     1501 tctgggatgg gctcatactg tcaatcccct tgatgcatcc gaccgttacg aattaaagct
     1561 tcaggatggc ggatatatac tggacgggca gataaagcaa ttcgcacaaa aaaactacat
     1621 tatcagggta aggcaggatg acggaagcta tgatgaaatt cctttctcag taaaatattc
     1681 agtacacgga ccggtggcag aatcggatga tggcagggca atggccatca gaattgccgg
     1741 tctggaggat ttcggaatat ttgaacagta tcacagaatg gcagcagcaa aaaattttac
     1801 cgggtttgaa tctgccctcc ggctgatgca gagccctatg ttcaatgttg tctatgccga
     1861 cagggacgga aatatctttt accttttcaa tggaaatatc ccggtaaggc aggagggtga
     1921 tttcaatttc tggcggggga ctattgacgg tagttcctca aagtacctgt gggataaata
     1981 tcatgactat gatgatcttc ccaggctgtt taaccctgaa acaggatttg ttcagaactg
     2041 caatgaaccg ccctggttct gcacatatcc gccggtgctg aaacagaccg attatccgcc
     2101 gtatttttca tcatcctgga tgacactcag accacagcgg gcaataaacc tgatacgagg
     2161 ccatgagaaa atatcttttg aacagctgat tgatattaag ctgaacacag gcatggaggc
     2221 tgccgacagg tttcttgccg gactgattga agcatcctct tcaagcacgg atcccattgt
     2281 cctggaagcc gtaagggtac tcagagaatg gaacgggaaa acagatacag acagtcgcgg
     2341 tgcagttctt tttgctgcct ggatggatct tgttacgccg gatatgtata taagaaaatg
     2401 ggatccggaa gatcctgccg atactccttc aggtttgaaa gatccggaaa aggctgctgt
     2461 tttacttggg aaagccgcat cccgggtcaa agactcgtac ggttcccttg acgttccatg
     2521 gggtgagata taccgcttca gaaggggaaa tattgattat ccggcgaacg gcggaccgga
     2581 tcatcttgga atcttcagga ctatgtattt ccaaaaggat gttgataata aattccgggc
     2641 ctttcacggt gatacttttc atgctgtggt tgaatttggt gataatcccc gtgcgatggt
     2701 actgcaggct tatggaaatt cctcccgtaa gggcgacagg cacaatggcg atcagattca
     2761 atacctgtcg gaaaaaaaat tgcggccggc actcctcagc agaaaagatc ttcgtgaaaa
     2821 tacagaactg actgaaaaat ttaacaaata caaatagtct taatcatgaa aatctatata
     2881 aggaatacat tgcaatccgg tcgtttgcta tctgttccac atattttttt ctgtttaatg
     2941 attttaatga ccgctgcttt taatcccctg cagggacaaa aagccgggga gcagcagaaa
     3001 agaattacag gggttgtcag ggatgaaagc ggacaggctc tttcaggtgc agcagtcctg
     3061 gttgagggaa ccaccactgg cacatacact gatgcagaag gcagatacac aataatggtt
     3121 tcggatgata gccaggtact ggtattttcg tttatgggat atatgacaca gaaatttccg
     3181 gtgaaaggca gaacagtcat tgatgcggat atgaatccgg acacacagac gctggatgac
     3241 gttgtggtgg tgggctacgg gacacagaaa aaggtgaatg tttccggagc ggttgatgtg
     3301 gttactgcaa aagccatcga aaaaagggca gtagccaacc tgtcccagtc tctccagggc
     3361 atctcgccga gccttaatat aagtgtcggc aattcgggag gtgagatggg agcccggatg
     3421 gaaatgaaca tcaggggtgt gggctctata acgggaggat cgccttatat ccttgttgac
     3481 gggctggaac aggatttgaa caaccttaac cctgatgata tagaaagtat ttcggtgctc
     3541 aaggatgctg catcatcagc aatttacgga gccagggcag cattcggagt tgttcttgta
     3601 accactaaaa aaggcaggaa tgacggtatt tcaattaatt attccaataa ttattctttt
     3661 tcagcaccta ccattgtgcc tcattctgtc gattcgaaaa ggtttgctac ctatctgaac
     3721 gttgcttcac ttaatgatgg aaatcccccc ttgttccaga atatcatcct ggattttata
     3781 gaccagtatc ttgcaggcga gatagatttc tggaccgcac cctatccgct tgctccgcag
     3841 tactggctgt tgaatcaggg agcatgggca aacaccgact ggtatgacat aaattataaa
     3901 aaatgggtgc caaacacact gcacaatctc agtatcagcg ggggggacca gaaaacccag
     3961 ttttatgttt cgggctcaat gtttgatcag tcgggtctgc ttaatttcgg aaccgataat
     4021 tacggaagat acaccctgaa taccaagata aataccagga tacttgactg gctgagattc
     4081 aattttacca gtaaattcag caggataaat attgaacgcc caagttatga ccggaatctt
     4141 ctgtatgacc ggcttgcaag gcagtggccg acgaatgccc cgtattttcc caatgggact
     4201 cccaattatg aagctgttca ggtatggctt gaacaggggg gaatgtacag ggaaaaaaac
     4261 aatgaactga gcataattcc cggactggag atagaacctt tgaaaggctg gattatttac
     4321 tcaaacctgc gctggaaaat gaacaactgg aacatgtcag accatgaagc aaaagtgaaa
     4381 gcatggctta ctgatatgtc aatgacatac ctgcgtccgg aaaataatta ttcaacaaac
     4441 aattatgcgg gatattatat gtcgcccaat atttacagca gctattcaaa agcatttgga
     4501 aagcataatt ttacagtgat ggcgggcttt gagcaggagc ttttcaatta tgaatcgacc
     4561 tttgcaaaga gatacgatct tataagcgat gaggtgcctt ccctgggaac tgccaccggc
     4621 agacaggaag tggcaggaac acagggccat tcggctacaa gaagcttttt cggccgtttg
     4681 aattatggat ttaatgaaaa gtaccttctg gagttttcat tccgatatga cgggtcttcc
     4741 aaattccccc cgggttaccg ctggggtaca ttcccgtcag gttctatggc ctacctgatt
     4801 tcgagggagg acttctggaa acctttatcc gtagtggtga accagtttaa gataagagct
     4861 tcctttggtt ccctgggaaa ccagaatgtt gacaattatc tctatataga aagattgcct
     4921 gtatcaacaa accttgccta catcatgggc aatgaacggc ccaattatgt gggcatggct
     4981 gcaccgataa gtccgggact cacctgggaa aaagtaaata caagcaatgc aggcatagat
     5041 gccgggtttt tgaagaacag gctgaacctg agtatggatt atttcatcag gaacacgcat
     5101 gacatgcttg gtcctgcaga atctctcccc gccgtccttg gaactgctgt tcccagatcg
     5161 aacaacgctg ctctcagaac aagaggtttt gaactttctc tcgactggcg ggatcatttt
     5221 gcaggtttta attacggggc aaaatttgtc ctctcggatg cagtctcaac agtaaccaga
     5281 tatttcaatc cggagaaact tctcagtgct ccctattatg aagggatgaa actgggagaa
     5341 atctggggct ttaccactgt cggacttttc gagtctgatg aacaggcaca gtccgttgac
     5401 cagtcatact tctccgccga tgttctcagg gccggtgacg ttcactacaa ggatatgaac
     5461 aatgacggca gaattgatat agggaaaaat actgtggcag accccggtga taagtcaata
     5521 atcggcaatt caactccgag gtttgcctat agccttaccc ttaatgcaga ctggaagggt
     5581 atcgatatga acataatctg gcagggaata gcgaaaaggg atctctggct cagtagtccg
     5641 attttctggg gggccggagg aatatactgg ctcacggcct atgaaggaca tatggattat
     5701 tggacgcctg aaaacacaga tgcgtattgg ccgagaccat atattgacca tggaagcaaa
     5761 aacaaacagg ttcagaccag atacctgcag acaggtgcct ttctgaggct gaaatccctt
     5821 cagcttggat acacaattcc gcagaagctt acaaagaaat tgtacataag caatttacga
     5881 atatatacct cgggagagaa tctgcttact tttacaaagc tgtttgaagc gtttgatcct
     5941 gaggccactg agggagaaag agcacacgga tatatttatc cccttcaaaa agtgttatcg
     6001 ggaggggtaa gcattacttt ctgaaaccgt catgattata aagatgaatt tcaaccatca
     6061 tttaacagtg agagagatga aaaaaagatt cttttcatta tttatactgg ccggtcttct
     6121 tgtttcctgt tcagaggatt acctgaaccg gacacctctg gatgagattt cacccggcga
     6181 ttttttcagg acggcgaatg atctcaggct ctatgccaac agattttatc cacttttgcc
     6241 gtctcattca gggcacgggg gaggaacctt ctggacagat gacaacagtg ataatcttgt
     6301 tccgggaacg gaggataagc gtctggccgg aacaagaact gtaccctcat cgggaggcgg
     6361 ctggaactgg gaacatatca ggcaggtaaa ttatttcctt gatcattgtt tcctggatcc
     6421 ggatgaggca gatcttaaaa ggacatatat tgccgaagtc aggttattca aggcaatgat
     6481 ttacttcgat atgctcagga ctttcgggga tgttccctgg tttacgaggg ccctgaatcc
     6541 cgattcgccg gaattatatt ccggaaggct cagcagggaa atcgttgcag attcaattat
     6601 agctctgact gattatgcca ttgaaaacct taaaccggcg ggccagtcag aggaattcag
     6661 acttaacagg gaggttgcaa tccttttcaa ggcaagggta tgtctctacg aaggaacctg
     6721 ggaaaaatat catcagggta cgcctttcgg tgttcagggc agcgacggga ccagatttct
     6781 gcaaatggca gaggaggctg cggcccttct gatgcagtca ggcaccttaa acctctacaa
     6841 aggtccggaa gggaaagagt attcttccct tttcaacaaa ctcgattatt ccgggatccc
     6901 ggaagttctt ctctgggaaa aatatgacat cggtctgggt atatatcact ggtgcagcca
     6961 gtatctgccc ttcggtgcag gtaataccgg tgtgtcaaaa agcctcatgg atgcctatct
     7021 gtgcactgac ggcaagccaa tctcagtaag ccctcttttc cggggttatg attcaataca
     7081 ggcagaatcg gctgatcggg atccgcggct ggctcagtca atgcttctgc cgggcgatga
     7141 tgtgacaatc aattctccgg gtggctcact tgataagaag tggtgggcac ccgcacttga
     7201 tcagagtcag attttcaggg caacaacggg ttattgtatg aataagggag caaatctgga
     7261 ttattcgcag cagcaaagtt cgggtggaat catgggcagc ataatcttcc gttatactga
     7321 agcgctgctt atatatgcag aggcaagagc cgaactggga attattacac aggatgatat
     7381 tgataaaacg gtcaataaga tccgggatcg tgttggaatg attcatctgg atatcaataa
     7441 tattaccatc gatccggcat gggatttccc tgaattgtca ccactgatca atgagatcag
     7501 gagggaaagg agggttgaac tggcctttga ggcccagaga tgggatgatc ttgcccgctg
     7561 gagggcacac aggctgcttg caggcaaacg gcccagggga attaaatata tcggatcaaa
     7621 tcttgaagga acctacaagg attatctggg caagccaacc atagtaatcg ggcagaatct
     7681 gtttgtagat tctgaaggcc gggttgaccc gtatcaggtc aaattcccca acggactggg
     7741 atttaatcct gaaagggatt atctttcccc cataccgagc gatgaactaa ctctgaataa
     7801 taaactaata cagaatccgg gatggtaaga atcattgtaa tatttctttt gcttgctttt
     7861 ttgccgggct gtgtgaaaca tacagtcacc ctggaaaagc ttatttccga aatgacttcc
     7921 agggaatcgc tatcctacta tcccttgccg gattacaaga tggttcaggc aagcagttat
     7981 aacaggaaat ccgtttcacc cgacagtgcc ggatggtttg agaacaacga catgtctcac
     8041 ttcataaggg tggaggaaaa ccgcggacga agggaatttg tcatgctcga cactgaggga
     8101 ccgggagctc ttgtaagatg gtggatgacc tttttcaagg cccgggacgg tgtgatccgc
     8161 atatatcttg acaattcaga agaacctgtt atacagggag ctcctggtga actgctgagc
     8221 ggaacacttc tggctccatg gcctttttct gcatccgtac aggaaggtgc accgctgggt
     8281 gaggaaggcc gtgattatga ccataacctg tattttcctg tccctttctc caggcattgc
     8341 aaagtaacct atgagtgtga ttcactaagg attctttatg attatgaagg attaagtgtg
     8401 ccggaaggat attactggcc cgatgtgttt tataatatta actaccgcct ttatgacaaa
     8461 tctgtaaaag tgcagtcatt taccctgaag gagctggaga aatgccggaa aaagcttgaa
     8521 actgccggct cttatctctt gtcagaagcc tctttgccgg gaaataagat ttcagcaaag
     8581 gagcggatta tgccgggtga ttccctcgta ctttctgatt gttccggtcc gtccgcaatt
     8641 ggcttccttc aggcagaaat cagctcttcc ggactgaatc agtccctgag atcggtggtg
     8701 ttgagtatca ggactgacgg tatttcttct gtccgggtgc ctgtcggtga tttctttggt
     8761 tccggctata cggtgcaaac gcaccgtacc ttttttaaca gcagggattc cgccggattg
     8821 atgaaatctt tctggatcat gccttacagg gaaagctgct ctattgcatt tattaattat
     8881 ggcagggata ctgttgagat ttccgcactg gcaggtacga ttccttaccg gtggaatgac
     8941 agaagcatgt atttcggggc gtcatggcat gaataccatc gcctcagaac acgcgatgaa
     9001 acagggtcct cctttgatct cacctttgta aaattggaag gcaggggtgt ttatacggga
     9061 gatcagcttg tgctttacaa taatacctat cactggtggg gtgagggtga tgagaaaacc
     9121 tttgttgaca atgaatcttt tccatccata tttggtaccg gatcagagga ttattacgga
     9181 tattcattcg gacgcaggga atcattctca catcctttca tttcacagcc ggtcggtaca
     9241 ggcaatatga gcaagggagt tacaataaac atgagacaca ggacactcga tgcaatacca
     9301 ttcaggacag caattgattc acggatagaa atgtggcact gggctgatat taaaatgaat
     9361 attgccctta catcctatta ctactttttt gctccgttga ccggaaacat agagccggac
     9421 attgtttccg tacaaaacaa ggtggctgtc accggaagcg attttgatga ggctgataac
     9481 taatggttcc gggaccctga caggattccg gaaatgaaac cggttttgct gtcccataat
     9541 gtattctgac atgccatgcg acagaatatt ttgcaacaag atttttttaa taagcagatt
     9601 atcatttagt tattatgaaa aatattcggg atgtgccaat acatttttta ttggctggct
     9661 ttatattttt ttcgttaagc tcctgtggca gaaaaagtga cattgtcacc cagggctctc
     9721 tttttgatga gatgatcagc ctttccaggc tgtcttcagc ccggggaacg aactacaggg
     9781 ctgttcagtt ttcatcatat gaccgcagaa gcgtcagtcc cggtgacagc acctggtttg
     9841 ccaatgaaga tggtttcgga ggagagccga tacccggatt cgaaaaagtg cttattgcac
     9901 ccgactcttc cggcagggga acgtatctga tctgtgatgt aagcggaccg ggagtgattc
     9961 agagattctg gacagcgggt atttccggta ctgtcagggt ttttctggat tctccggacg
    10021 aacttttata tgaaggcagt gcagaggatt ttttcaggaa gccggctgag acacttaccg
    10081 gggacaatga ccttgcaaag catactcatt ctttttacca gtttgatgct tcctacttcc
    10141 cggttccgtt ttcaaaaaga ttcagaatgg aatggacggg gaataccagt gatattcatt
    10201 tctatcatgt cggacttcgt ctgtataaca aaggtcagaa tgtggaatcc ttcagcaggg
    10261 agaatttcat caaatacagg tcagcaatga tcagaacaga ttcactgctc agaaacccgg
    10321 ataacaggcc ggccgggaag cgttattctt cagttaacga gacaatcaca ctggaatccg
    10381 gagaaaaggc cctgctcatg aatcttgagg gaccatctgt gattacccgc ttcaaggtga
    10441 agatcaatgc cgtcaacccg gaaaatgcac taaggcaggc agttctgaga atatggtttg
    10501 atgaaacaga aaatgcacag gtgaattctc ccctgggtga ttttttcgct gccgcaccgg
    10561 gaatcaatcc ttatgaatca cttcctttct ctgtgctgac tgacagtacc atgatgtgca
    10621 ggtttgaaat gcctttcagg gaaaacgcaa aaatcgcagt tgagaactat tcctccggta
    10681 aaatagaaat aaatgcagaa gcaattgttg ccccgtttag atggaaagag ggagaatcga
    10741 tgcatttcca tgcaggctgg agtatggatc acggcctgac aacttcagaa cttgattcca
    10801 gggcttcgga tataccttat ctcattgccg ggggcagggg gagaattgca ggagcagccg
    10861 cactgatcca caatccgagc agcgtgccca cgtcgtgggg taactggtgg ggggagggtg
    10921 atgagaaaat attcatagac agggatactt ttccttcctt tttcggaacc ggttcggagg
    10981 attatttcaa ttattcctgg tcgtcttcag agatattctc ttatccatat tgcgggcagc
    11041 caagaaatga tggaccgggc aacaggggat atgtttcaaa ctacagatgg catatcgccg
    11101 atgatattcc tttcggggag aaagtaaaat tttatatgga actgaaacat catggcagag
    11161 tagaaggatt ctcctacggc cggatcattt acttttatct tctgcccggt gataataatt
    11221 tgccagcaat gttttctgaa aatgatctga aagaaataag gtacgatcca tggcttccgg
    11281 aagctttcaa aggttcagca ggatggagct ttgtccaggc tgaagaactt gtgtcaggca
    11341 ctgaagatat ttgcattgaa aaagataatt tctgggccgg ttccggagtt ttgaaatgga
    11401 agcccgcaca tcatcaggat atcctgaaac tgaatattca ttcttccggg gaggtcagag
    11461 aaacacgtat tggcctaacc ctgacacatg acccgcaatc gggacgcctt gttttcagat
    11521 taaacggaaa accggtcagg ttcagcggca gcgataccct ggacatcagc acccgttccg
    11581 ggagactgct ggagaaccac ttttcctctc cgctcagcct gagaaaggga ttaaatgaac
    11641 tggaaatcag tatgcccggc gctgatggcg gaaaaattgc gaaaattgac tttgtctggc
    11701 tgagagagtg agatatcctt taaatcaata attaatatac cccgccatgg aaaaaagcat
    11761 gaaaaaaaca gggattgtca ggattatatt gctgacaata ataattctgt tccaggtttt
    11821 accgtccccg tgtcaggaca gggaaaggga attcaacggc tggtattacg ggagaatgct
    11881 tgacagaatc agctttcccg tcggaggtct gggagccgga atgttctgca tggatggcaa
    11941 cggatcagtt tcgcatctgt ctgtcaggca tcatcccgat ctgtttaacg agccggtttg
    12001 ctttgctgcc atatcagtaa aagattgtga aaacggagcg aaagttctcg aataccaggt
    12061 gccggaatgg aaatattacg gtcggcccgg agccggactc ggattcccgg ggaagcttta
    12121 cggcctccca aggtttgaaa aaggccgctt tctggccagg tttccctttg ccattcttga
    12181 gcttgaagac gaagacattc ccctgtctgt ggaactgaaa ggatggagtc cttttattcc
    12241 cggtgatgaa gacaattcaa gccttccggc aggagtattt gaatacagtt tcaggaacaa
    12301 tactgacaga acccttgaag ctgttttttc ctggaactca aggaatattc tcactgaatg
    12361 ggctggtccc gtcaggggta taaaaggcgg ctttgtcatg tcggaaaaca gttctgacga
    12421 aaacacggtc agaaataccg ggtttgccgt ctttacagac gacccttcag caatagtcga
    12481 tcactgctgg ttcaggggac agtggtttga tccctatatt attctctgga agaatattga
    12541 aacacaaacc atcccttcca atccggaaag cagggagccg gttggaggag cttcaatcta
    12601 tattcctttc atgcttgatc cgggtgagga aaaaactgtg aaacttaatt tctgctggta
    12661 tttgccggaa tccggactgt caataggaaa tattccggga acagacgaga aactgccgcc
    12721 ctatgaagtc tggtacagtt caaaattcag atcgctggga gaggtgattg attactggaa
    12781 taccaacagt gaaagactca ggaaagaaac ggagctgttc accaatgccc tttattcatc
    12841 caccctgcca tcagaagtaa tggaagctgt tactgccaac ctgagcatcc tcaagtcacc
    12901 aaccctgctc aggcaggcgg acggaaggct gtgggcctgg gaaggctgtg ctgacagcga
    12961 gggcagctgc cacggaagtt gcacacatgt gtggaattat gcccaggcta ttgcccatct
    13021 tttccccggg cttgaaagaa ccctgcggga gacagaattc cttgtcagtc aggatgctga
    13081 aggccaccag aatttcaggg tcaatctgcc ggtttcggaa ccgccccatg gttttcatgc
    13141 tgcagccgac ggacagcttg gaggtatcat gaaagtttac cgggaatggc gtataagcgg
    13201 gaatacggaa tggatgagga atctttaccc gaaagtcagg cagagccttg attattgtat
    13261 aaaaacctgg gatcctgatc ataaaggata tctggaagaa ccgcatcata atacctatga
    13321 tattgaattc tggggagcca atggtatgac aacaagcttt tatctcgggg ctctgaatgc
    13381 cttaattgaa atgagcaaag ccctgaaagc gccttataaa gattatgaag ccattctcaa
    13441 gaaagggatt tattatgctg atactgaact tttcgacggg gaatactata ttcagaaaat
    13501 aaaatgggag ggccttcatt ctcctgatcc tgttgaagca gccaggaaaa gcttccagac
    13561 aaactattct cctgaagccc tcctgatact caaggaggaa ggaccaaaat accagtatgg
    13621 atcagggtgt ttgtcagacg gtgtcctggg aatgtggttg tctcaggttt gtggtttaag
    13681 gcaggttatg gataattcaa aaataaccag ccatcttgcg tcggttcata agtataattt
    13741 aagaaataat ctgacggatc atgcaaatcc gcagcggccg gcttatgctg cagcatccga
    13801 gggcggattg ctgctctgct catggccgaa gggaggcagg ctcagtttgc cgtttattta
    13861 cagcaatgaa gtatggacgg gcatagaata tcaggctgcc agccaccttt tcttccaggg
    13921 agaggttcag aagggcctgg atattgtgag aaccgcaaga ctcagataca gtggaaatat
    13981 aagaaatccc ttcaatgaga tcgaatg
//
LOCUS       DAOSPX010000049        13654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4584_length_13654_cov_4.530774, whole genome shotgun sequence.
ACCESSION   DAOSPX010000049 DAOSPX010000000
VERSION     DAOSPX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13654
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4584_length_13654_cov_4.530774"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLZ75_04030"
     CDS             complement(<1..336)
                     /locus_tag="PLZ75_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71600.1"
                     /translation="MNSFREKSRILRFQLLALIFVPAIVSSQSAKEIREIYKQAEAHY
                     LYEEYDLANQLYILLDDPENMNLKYKIGTCYLNIPDEKEKAIPFLEEAVKNAACDAKI
                     KSLKEKRAPL"
     gene            complement(541..2658)
                     /locus_tag="PLZ75_04035"
     CDS             complement(541..2658)
                     /locus_tag="PLZ75_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS71601.1"
                     /translation="MIKRVLPFIIFLILINSSNLVSQSRTSNRINFREAESWILFEDY
                     REALPIYQELLKLYPDNSNFKYRIGQCYLNLPGEKEKAIAYLEEAADNINPDYKEKNF
                     REKGAPWDALYYLANAYRINLQLDKAIETYRLFMEKLDPRIYDSAIVAFQIRSCENAK
                     VLMASPVFIKEENLGRVINDDNSEFNPVVSADETLLVFSRRLPFYDAIMYSVRENGSW
                     TEPRNMNEILKIDRNIYPSSVSSDGKVLYLYNSDNYDGNIYTSTYENGTWGPAVKLND
                     NINTKYWESHAAVSHDNKKLFFTSNRKGGYGGLDIYVSDRDSTGDWGPAVNLGPVINT
                     RFNEETPFLSRDGKTLFFSSSGHHNMGGYDIFYSSLLGNGEWSVPLNAGYPLNTTDDD
                     LFFMPLNQGYEGYYSRTGPQGYGGQDIYRVEIFSDKHPRKFRVSGMAKIDGPDMRPAN
                     KIRISSFNIRNPEEISEGLANPETGSYELTVNHGDHTLIYETNGREALRKNIHLPLAS
                     KSDSFILPPAILPLAGSRAILGSRMESNINAYERDSILIPLKTEPKSRLVAEHWHGDS
                     LLHTRRFNIDDSVFNLKISPRRGGNRILLTLSNQYGNTAEKEFFITAEKKEERAWIRR
                     PEHPEILAERRAEAISRMEGKIRTVPAETSENLEGDPDSTSGTVPQEKKSCRLWYLWI
                     IAGAGIAWFVLVYYKKRKNKNEG"
     gene            complement(2864..4228)
                     /locus_tag="PLZ75_04040"
     CDS             complement(2864..4228)
                     /locus_tag="PLZ75_04040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOS71602.1"
                     /translation="MAKIDILLPAMGEGVIEATITKWLVKEGSVVREDDPLVEVATDK
                     VDSEIPAPADGKIIKITAPAGTVVQVGQTIAVIESDALAAPDEPGILQEVERIRETIS
                     EVRREKQELDNVSRNLKSRTPSGKFLSPLVRSIAARENISYSDLDRIKGTGMDGRITK
                     DDVLGFLESLKGKKEIAQTGATGYKSPAAAGKEESEETLSATGKTPKAAGQAAASGDS
                     GDEIIEMDRVRKIIAEHMVLSKKTSPHVTSFIDADVTKLVNWRNSVKDRFLASAGYRL
                     TLTAVFIDATARALSELPMLNISVDGNNIIKKKNINIGMATALPDGNLIVPVIRNADE
                     KNLNGLARSVSDLAERARNNKLKPDEITGGTFSLTNFGSFDNLAGTPIINQPQTAILG
                     TGAVRKIPAVIETPDGDVIAIRHVMILSLSYDHRVIDGALAGRFLKKIKEILENYTPD
                     LILH"
     gene            complement(4320..5633)
                     /locus_tag="PLZ75_04045"
     CDS             complement(4320..5633)
                     /locus_tag="PLZ75_04045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS71603.1"
                     /translation="MPGGSLNQTENNMVPPADLSVIIVSYNVKYFLAMCLASVRKAGR
                     NLNCEIFVVDNNSSDGSCEMIQRDFPGVKLISNRENRGFSAANNQAIKEAGGKYILLL
                     NPDTIVEEDTFTRCIRFMDEHPDAGAAGVMMINGHGKYLPESKRAIPLPETAFWKMSG
                     LYRLFPASPRINRYYLGHLNKLDINRIEVIAGAFMFLRSKAVSETGLLDEDFFMYGED
                     IDFSYRLLGKGYENYYFPDVRIIHFKGESTKKEEMDFVIRFYDAMLIFVRKHFSSTTL
                     RKMILPVSTAIRLRAAASVIHRWFRRMLDPLTGRITRVIGPGRNSPAEGIKTAVLSGA
                     DMLEKINYLLAVTGAKADVVISTDKYMTDPGGSIQEIIQRTAESIVSKKVRQVIFSPA
                     NMEFSSIIDCMQHLSQHRIRFRIASPDGKYLIGSGSAVSADGRGS"
     gene            complement(5659..6273)
                     /gene="recR"
                     /locus_tag="PLZ75_04050"
     CDS             complement(5659..6273)
                     /gene="recR"
                     /locus_tag="PLZ75_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOS71604.1"
                     /translation="MSFTEFPSRLLENAVNEFASLPGIGRKTAFRLVMNLLRRDQGEV
                     TRFGESIIRLRSEIKYCSVCHNISDTEICSICSDSKRDRSTICVVENIQDVMAVENTR
                     QYRGLYHVLGGIISPIDGIGPADLRIDSLEAKVQSGNINEVILALSATMEGDTTNFYL
                     YRRLGKYNTLITTLARGVAVGDELEYTDEITLGKAINNRNPYQQ"
     gene            6417..7868
                     /locus_tag="PLZ75_04055"
     CDS             6417..7868
                     /locus_tag="PLZ75_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOS71605.1"
                     /translation="MSPYLIAGIFLVYTALLFLITWITARKADSQSFFTGNRASPWFV
                     VAFGMIGASLSGVTFMSVPGWVSETQFTYMLVVFGYLAGYFVIALVLLPLYYRLQLTS
                     IYTYLEKRLGSWSYRTGAGFFLLSRTLVTSLRMFLVINVLQLFVFDAWSMPFWLNALV
                     FIILAVLYTFRGGIRTIIWTDTLQTGFMLLAVVLSMVFISRELDAGFGSLVMEIRRAG
                     MSKMFVTDWHHERYYLKQFLSGMFITITMTGLDQEMMQKNLSCRNIREARKNMFTFSG
                     ILILVNFLFLLLGAFLVLYADKRGISVASSDDLFPTIAFKYMSPLAGIVFLTGLISAA
                     FPSADGALTSLTTSVSIDFLRLDRREDLTEKKATGIRYIVHLSAAILIFACIMIFRIV
                     NDRAVIDMLFTLVSYSYGPLLGLYTFGLFTRRKARDRLTPLIAVLSPVICYFLSENSV
                     LLLNGYRFGFELIFLNGFLTFAGLLAFSRTKAD"
     gene            complement(7942..8784)
                     /locus_tag="PLZ75_04060"
     CDS             complement(7942..8784)
                     /locus_tag="PLZ75_04060"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HOS71606.1"
                     /translation="MTLRTGQATRWITVLRTIMTFCFIMTLFLLPGTAFSQAGGNAEV
                     KDSVDIPLKIRTGIDITGPAIWFTDKSILTAEAYISTDLNEKFALFLGAGYSDYSYSQ
                     YNYDYLSKGSFFKAGMDFNLLRPEIAAGKYWAGIGIRYGISSCLSETPSFTHENYWGS
                     ISSAIKPYRIMGHYLEISPGFRAELFKNFSIGWSVNLRKLISPGQKKDLRPLWLPGYG
                     SGESVTAGAAYYISWNIPYRKIRVGLKKDEPEEYYDDDESGGSREDRSYRSISGSGAG
                     NRLR"
     gene            complement(8846..9310)
                     /locus_tag="PLZ75_04065"
     CDS             complement(8846..9310)
                     /locus_tag="PLZ75_04065"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HOS71607.1"
                     /translation="MRNTFNIISAVVVACALFLSVSCTPGSCFEETNSYLKATFYRTS
                     QGKPVPPDSLSMYGIGKDSAVYKKASRVQPALIPLDPSASACGFLIRINGVDDTVSFF
                     YSTFTHLISRECGYTFFHDLDSLAYTRNIIDTILIKFRTISTFNEPNIFIYY"
     gene            complement(9366..12002)
                     /gene="alaS"
                     /locus_tag="PLZ75_04070"
     CDS             complement(9366..12002)
                     /gene="alaS"
                     /locus_tag="PLZ75_04070"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOS71608.1"
                     /translation="MKSQKLRQLFLNFFREKGHEIVPSAPMVIRNDPSLMFTNAGMNQ
                     FKDIFLGHTAPKYRRVADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWEFLNGRLGIPADRLYATIFTGSPEDNVPRDEESFRWWQECFPGQSDRILE
                     GSKEDNFWEMGETGPCGPCSEIHVDLRDDSEREKIPGHSLVNRSHPLVLEIWNLVFIQ
                     YNRRADGSLELLPDSHVDTGMGFERLCMVVQGKTSNYDTDIFQTLINEISISCGRKYG
                     ETEEQDIAMRVVADHLRAISFSIADGQLPSNNKAGYVIRRILRRAVRYAYNYLNVDEP
                     FMYRLVPVLVAEMGDQYPELKNSEEHISRIIHEEESSFLKTLGKGLKMIDRMISDLKM
                     KKINVLPGRIAFELYDTYGFPADLTRLILRENNMTLDTEGFEQEMENQKNRSRKDGTL
                     DSGDWINVRGGDTTIFTGYDKTTDDILIMKYRVVKARGKDICQLVFDKTPFYAESGGQ
                     SGDSGVIASEEEQIRITDTIWENELIIHLADKIPSRPDAAFVAKVDLRKRAMTASNHT
                     ATHLLHYALRKVLGNHVEQKGSLVTSERLRFDFSHFSRLTREEILEVENIANQLVRNN
                     FTRQVMDDVPLEEALTMGAMALFGEKYGDTVRVVRFGDSVELCGGTHADSTAGIGLIK
                     IISEGAIAAGIRRIEAITGSKADEYINEKIAELENVASMLKSTGNVRESVEKLISENA
                     ALRKTVEKYKSRIAKDIIRDLVQNAVQIGDVRFIRGQVEADSAETLKSIAFEIRKIPG
                     KNVAVMGSQIGEKAGLVAMVSDPLAKEGRLEATEIIREIAPEISGGGGGQPFLATAGG
                     KNPGGIARALEKAGKFIRERLS"
     gene            12126..13088
                     /locus_tag="PLZ75_04075"
     CDS             12126..13088
                     /locus_tag="PLZ75_04075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS71609.1"
                     /translation="MIKKKYYLDPNDLQYKRLRLPWKRKFLMGLLWFTVTVVLTVIYS
                     TIFHSIFGSPKYQALSRQLSALKLNFTLANRELDLTVDMINDLKMSDEVRYRPVLAME
                     SLPESYRNPGFGGIDRFRDLNGYPSSSIMRNTRTRIEEMKNMLEVQQASFRSIIEREA
                     EWSRMYSYLPLICPVSVKIRRGEGVMYREVHPVLGTSQWHHGQDFRTPYGTEVYATGN
                     GRVIEAGWNSGGFGNYVVIDHGYGYQSTYAHLSGIKVSKGMEVKRGDLIGLTGNSGIS
                     TGPHLHYQIDLYGRYQNPLHFFNDDLTEDEYFEMINLLTSSKKF"
     gene            13178..>13654
                     /locus_tag="PLZ75_04080"
     CDS             13178..>13654
                     /locus_tag="PLZ75_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HOS71610.1"
                     /translation="MPKSKYKFNPDSLSFDKVTLRAKDIFLRLMAFFVGSLLIAVLYY
                     LIFALFFNSPKEKALIREIEQLALQYEIIQRDMGNMEKVIVQLQETDDNLYRTIFEAE
                     PIPVTLREGGVGGVNRYKELEGFSNSNIVIETTRKLDKLRKQIYTQSKSFDELIILS"
BASE COUNT         3218 a         3188 c         3125 g         4123 t
ORIGIN      
        1 aagcggagct cttttttcct tcagggattt tattttagca tcgcaggctg cattttttac
       61 agcttcctcc aggaatggta tggctttctc cttttcatcg ggaatattca ggtaacatgt
      121 tccgatttta tatttcagat tcatgttttc cggatcatcc agcagaatat aaagctggtt
      181 ggccagatca tattcttcgt acagataatg agcttcagcc tgtttgtata tttcccttat
      241 ctcctttgca gactgggatg agacaatggc aggaacaaaa atcagagcca gcagctgaaa
      301 tctcaggatc cgtgattttt ctctgaaact attcataata acaaatgtag cgttttataa
      361 tgtcttaatc cagatgaatt ttcaatattt tactcaggta ttttttttat taacggagat
      421 tgttttcctg cagcagggtt attgttccgg ccgaagaaca cagatgtctc ccggctgtaa
      481 tttattggaa gcccctgacc gcagtttgtc agaaccttca gtccgcagct ttgttccgca
      541 tcagccttca tttttattct tccttttttt gtaatagaca agtacaaacc atgcaattcc
      601 tgctcctgca ataatccaca aataccacag cctgcagctc tttttttcct gcgggactgt
      661 tccggaggta ctgtccggat ctccttccaa attttccgat gtttccgcgg gcaccgtcct
      721 gatttttcct tccatcctgg aaatagcctc ggctcgtctt tctgccagta tttcaggatg
      781 ttccggtctt ctgatccatg ctctttcttc tttcttttcc gctgttatga aaaattcttt
      841 ctcagcagta ttaccgtact gattactgag agtcagcaaa attctgtttc cgcctctacg
      901 cgggcttatt ttaagattga aaaccgagtc atcaatatta aagcgccttg tatgaagcag
      961 tgaatccccg tgccagtgtt ctgccacaag acgtgatttt ggctcagtct tcagagggat
     1021 caggatgctg tctctttcat atgcgtttat attgctttcc attcgtgatc cgagaattgc
     1081 acgtgatccg gcaaggggca ggattgctgg aggcaggatg aaggaatctg atttgcttgc
     1141 aaggggcagg tgaatgtttt tcctgagagc ctccctgccg tttgtttcat agatgagggt
     1201 atggtcgcca tggtttactg tcagttcata tgagccggtt tcaggatttg ccaggccttc
     1261 ggaaatttct tcaggatttc tgatgttgaa tgagctgatt cttattttat tggcgggcct
     1321 catatcgggg ccgtcaattt ttgccatgcc gcttacccgg aatttccgcg gatgcttatc
     1381 cgagaatatt tctaccctgt aaatatcctg accgccatat ccctgagggc ccgttcttga
     1441 ataatagcct tcgtagccct ggttcagcgg cataaagaac agatcatcgt cggttgtatt
     1501 caggggatat cctgcattca ggggaactga ccactcgccg ttgccgagga ggctggagta
     1561 gaaaatatcg tatcctccca tgttatggtg tccggatgag ctgaagaaaa gcgttttgcc
     1621 gtccctgctc aggaagggag tctcttcatt gaatctggtg ttgatgacag gtccgaggtt
     1681 gactgccggt ccccagtcac ccgtggaatc cctgtccgaa acgtaaatat ccaggccgcc
     1741 gtagccgcct ttcctgttac tggtgaaaaa gagctttttg ttatcatggg aaacagcagc
     1801 atgggattcc cagtatttgg tgtttatgtt gtcgttaagc tttactgccg ggccccacgt
     1861 gccattttcg tatgtgcttg tataaatatt tccgtcatag ttgtcggaat tgtagagata
     1921 gagaacctta ccgtcggatg agactgagga agggtagatg ttcctgtcga ttttcagtat
     1981 ctcattcata tttctgggtt cggtccatga tccgttctcc ctgacggagt acataatcgc
     2041 atcatagaag ggaagccttc tggaaaagac cagcaaggtt tcatcggcag aaacaacagg
     2101 gttgaattcg gaattgtcat cattaatgac tctgccgaga ttttcttcct ttatgaaaac
     2161 aggggaagcc atcaggactt tggcattttc gcatgaacgg atctgaaatg ccactattgc
     2221 cgaatcatag atcctggggt caagtttttc catgaaaagc ctgtatgttt caattgcctt
     2281 gtcgagctgc agatttattc tgtatgcatt ggcaaggtaa tagagtgcat cccacggagc
     2341 gcccttttcc ctgaagttct tctccttgta atccggattt atgttgtctg cagcttcttc
     2401 cagataggca attgcctttt ccttttcacc cggcagatta agatagcact ggccgatcct
     2461 gtatttgaaa tttgaattat ccggataaag tttcagaagt tcctgataaa tcggaagagc
     2521 ttccctgtaa tcctcaaaaa ggatccagct ttccgcttcg cggaaattaa tcctgtttga
     2581 tgtccttgac tgggatacaa gattgctgct gttaataagg atcagaaaga taatgaacgg
     2641 caaaaccctt ttaatcatac tgaaacagac ataaatggtt tgactaaacc tgaaaaacaa
     2701 ataatgctaa aacctgatta cgacagtata ctggccccgc aataaattat gcgcaatata
     2761 ataaaaatgg acaagaacag cctgtccttt ttatcaaccg ttgttaacat cttctcaaca
     2821 atgcagacgg gcagactgat tctatgtcat cagattatgg atatcagtgc aggatcagat
     2881 cgggagtgta attctcgagt atctccttta tttttttcag gaatcgtccg gcaagggcgc
     2941 cgtcaatgac cctgtgatca tagctcagag acaatatcat gacgtgccgg atggcaatca
     3001 catcaccatc aggtgtttct attactgcag gtatttttct tactgctcct gttcccagga
     3061 ttgctgtttg cggctggttg atgatcggtg ttccggcgag attgtcaaag ctgccgaaat
     3121 tggtgagtga aaatgtgccc ccggtgatct cgtcgggttt cagtttattg tttcttgctc
     3181 tttcagcaag atcgctgaca ctgcgggcga ggccgttaag atttttttca tcggcattcc
     3241 tgatcaccgg cacgatgagg ttcccgtcgg gcagggccgt tgccattccg atgtttatat
     3301 tttttttctt tataatattg tttccgtcaa ccgagatgtt cagcattggc agttccgaca
     3361 gcgccctggc tgtggcgtca atgaagacag cagtgagagt aagcctgtat ccggcggatg
     3421 ccaggaacct gtccttgacg gaatttctcc agtttaccag tttggtgaca tctgcatcaa
     3481 tgaaggatgt tacatggggg gaagtttttt tcgagagtac catgtgttcg gctatgatct
     3541 tcctgaccct gtccatttct attatttcat ctcccgaatc cccggaggca gctgcctgtc
     3601 cggccgcttt gggggttttt cctgtggcag acagggtttc ctcagattct tccttcccgg
     3661 ctgctgcagg agatttatat cccgttgctc cagtttgtgc aatttccttc tttcctttca
     3721 gggactctaa aaatccaagg acatcatctt tggtaattct tccatccatt cctgttccct
     3781 ttatcctgtc gagatccgaa tagctgatgt tttccctggc cgcgatgctt ctgaccaggg
     3841 gtgaaaggaa tttgcctgag ggagttctgc ttttcaggtt acgtgataca ttatcaagct
     3901 cctgtttttc cctgcggacc tctgaaatgg tttccctgat cctttcaact tcctgaagaa
     3961 ttccgggctc atccggtgct gccagggcat cactttctat tacggcaatt gtctgcccga
     4021 cctgtaccac tgttcctgca ggagctgtga tctttataat tttgccatcg gcgggggcag
     4081 gtatctcgga atcaacttta tcagttgcca cttcaaccag agggtcatct tccctgacaa
     4141 cagacccttc ctttacaagc catttggtaa ttgttgcttc tataacccct tcgcccattg
     4201 cgggaagtaa aatatctatt ttcgccattt ctcggttaat tcttgttttt tgttacagaa
     4261 taacatattg aaatgaaaat tgttcggcaa tttttacttt gtgcgggcaa tggttcccgt
     4321 taactgcccc tgccgtctgc agagacggcc gaaccgcttc ctatcagata tttcccgtca
     4381 ggcgaagcta tcctgaatct tatcctgtgc tgcgataaat gctgcataca atcaatgatc
     4441 gaagaaaatt ccatgttcgc cggactgaag atgacctgcc tgactttttt actgactatg
     4501 ctttcagctg ttctctgaat aatttcctga atgctgcctc cgggatctgt catatattta
     4561 tcagtactga tcaccacatc ggctttggct cccgtgacag ccagcaggta atttattttt
     4621 tccagcatat ctgcccctga aaggactgct gtttttattc cctctgccgg cgaattccgt
     4681 cccggtccaa tgacccgtgt tattcttccg gtcagcggat caagcatcct gcggaaccac
     4741 cggtgtatta cagaagctgc agccctgagc ctgatggcgg tactaacggg aaggatcatt
     4801 ttcctgaggg ttgtgctgct gaagtgtttc cggacaaata tcagcattgc gtcatagaag
     4861 cggatcacga aatccatctc ctcctttttt gtactttctc ccttgaaatg gataatcctg
     4921 acatcgggga aataataatt ttcatatcct tttccgagca gcctgtagct gaaatcgatg
     4981 tcctcaccgt acatgaaaaa atcctcatcc agcaatccgg tttccgaaac ggctttgctc
     5041 cgtaagaaca taaaagcacc ggcgatgacc tctatgcggt ttatgtcgag cttattaaga
     5101 tgtcccagat aatacctgtt gatccgtggg gaagccggga agaggcggta gagtccgctc
     5161 attttccaga atgccgtttc aggcagggga atagctcttt ttgattccgg gagatatttt
     5221 ccatggccat taatcatcat taccccggca gcgccggcat ccggatgctc atccataaac
     5281 ctgatacatc tggtaaaggt atcttcctca actatagtgt caggattcag aagcaggatg
     5341 tattttcctc ctgcctcttt tatagcctgg ttgtttgcgg ccgagaatcc cctgttttcc
     5401 cggttggaaa tcagtttcac ccccgggaag tccctttgaa tcatttcaca ggatccgtcg
     5461 gaagaattat tatcaacgac aaagatctcg cagttcaggt tgcggcctgc cttcctgact
     5521 gaagcgaggc acatcgcgag aaaatatttc acgttgtagc tgacaataat tacagacaga
     5581 tccgcagggg gcaccatatt attctcagtc tgattcaggc ttccgcccgg catttccatt
     5641 tttaaagaga gtatttgttt attgctgata tggattcctg ttgttaatcg ctttgcccag
     5701 ggttatttca tcggtatatt caagttcatc accaactgca acgcccctgg ccagtgtggt
     5761 gatcagggta ttatactttc ccagtctcct gtacaggtaa aaattcgtgg tatcaccctc
     5821 catggttgcg ctcagggcaa gaatcacttc attaatattt ccggactgca cctttgcctc
     5881 gagtgaatct atcctgaggt ctgcaggtcc tattccgtca atgggggata tgattccccc
     5941 gagaacatga taaagccccc tgtattgccg ggtattttct actgccatca cgtcctggat
     6001 gttttccact acacagattg ttgacctgtc gcgcttgctg tcgctgcata tgctgcagat
     6061 ctccgtatca gaaatattat ggcatacgct gcagtatttt atttccgaac gcagtctgat
     6121 aatgctttcg ccgaaccttg ttacctcacc ctgatctctt ctcagcaggt tcatgacaag
     6181 cctgaaggcg gtctttcttc ctattcccgg cagggaagcg aattcattca ccgcgttttc
     6241 aagcagcctc gaaggaaatt cggtaaaact catgactaat gtttcaggca aaaataattc
     6301 aatttttgat tatgttgccg ggagttccgc ccggtcccgc ttttattacc ggaaagcgat
     6361 tctgtaattt ttaagctttc attcttttta ttattcttgc agatcattaa atccgtatgt
     6421 caccctacct cattgccgga atattccttg tatatactgc cctgcttttc ctgattacat
     6481 ggataacggc cagaaaagcc gacagccagt cctttttcac cggcaacagg gcatcgccgt
     6541 ggtttgtggt ggctttcgga atgatcggag cctccctttc tggggttacc ttcatgtcgg
     6601 ttccgggatg ggtttcagag acgcagttca catacatgct tgtagtgttt ggttacctgg
     6661 ccggatattt tgtaatcgca cttgttcttc tgccgctcta ttacaggctt cagctgacct
     6721 ccatctacac ctatctggag aaacggttag gaagctggtc ctacaggaca ggcgcaggtt
     6781 tctttctgct ttcacgcaca ctggtgacat ctctcagaat gtttcttgtg attaacgtgc
     6841 tgcagctctt cgtttttgat gcctggagca tgcctttctg gctgaatgct ttggttttca
     6901 tcatacttgc agtgctttac actttcaggg gagggatacg taccattata tggaccgaca
     6961 cccttcagac aggcttcatg ttgctggcag tcgtgctttc aatggttttc atcagcaggg
     7021 aactggacgc cggtttcggg agcctggtaa tggaaatccg ccgggccggt atgtcgaaaa
     7081 tgtttgttac agactggcat catgagcgtt actatctgaa acagtttctt tcaggtatgt
     7141 ttattaccat taccatgacc ggccttgacc aggagatgat gcaaaaaaat ctcagctgcc
     7201 gtaatatacg ggaagccagg aaaaatatgt tcacattcag cggtatactt atactggtga
     7261 atttcctttt cctgctgctc ggagccttcc ttgttcttta tgcagacaaa agggggatca
     7321 gtgtcgcttc gtcggatgac ctgtttccca ccattgcctt taaatacatg agtccgctgg
     7381 cagggattgt atttctgaca ggactgattt ctgcggcatt cccgagtgcg gatggtgcac
     7441 tcacttcgct caccacctct gtcagtatag attttctcag gctggacagg agggaggatc
     7501 ttactgaaaa gaaggcaaca ggaatcaggt atattgttca tctgtcagcc gccatcctga
     7561 tttttgcctg tatcatgata ttccgtatag taaatgacag ggccgttatc gatatgctgt
     7621 tcacccttgt ctcatacagc tatggtccgc tgctgggcct ctataccttc ggccttttta
     7681 caaggagaaa ggcccgtgac cgtcttactc cgctcattgc ggttctgtcg ccggtaatat
     7741 gttatttcct cagtgagaac tcggttcttc tgctgaatgg ttacaggttt ggttttgaac
     7801 tgatatttct gaacggattt ctgacctttg cggggttgct ggccttcagc aggacgaaag
     7861 cagattagaa cgggagatgc ggatcccggg aaagaacgtg acagggataa gcaggtaaca
     7921 gaggatatgc cgtaattatg atcatctcag acggtttcct gcaccgcttc cgctgatgct
     7981 acggtaactt ctgtcctccc tgcttccccc ggattcgtcg tcatcgtaat attcctcagg
     8041 ctcatctttc ttaagcccga ctcttatttt cctataaggg atgttccatg aaatgtaata
     8101 ggctgctccg gcagtgactg attctccgga tccgtatccc ggcagccaga ggggtcttaa
     8161 atcctttttt tgtcccgggc tgatcagttt ccgcaggttg accgaccatc ctatgctgaa
     8221 gtttttaaac agttccgccc tgaaacccgg ggaaatttcc agataatgtc ccatgatccg
     8281 gtatggtttg atggccgaac tgatactgcc ccagtaattc tcgtgagtaa aggagggcgt
     8341 ttctgaaaga cagctgctga tgccgtagcg gatgccgata ccggcccagt attttcctgc
     8401 cgcaatttcg ggcctgagca ggttgaaatc cattcctgcc ttgaagaaag atcctttgct
     8461 caggtagtca taattgtact gtgagtaact gtaatcagaa tatccggcac caaggaacaa
     8521 tgcaaatttc tcattcaggt cggtggaaat atatgcttct gcagtcagga tacttttatc
     8581 cgtaaaccat atagcaggac cggttatatc aattccagtc ctgattttca gaggtatatc
     8641 aacactgtcc ttcacttcgg catttccgcc tgcctgtgaa aaggcagtac ctggcagaag
     8701 gaagagagtc atgatgaaac agaaagtcat gatggtccgg agaaccgtga tccagcgggt
     8761 ggcctggccc gtacggagag tcatgatgca acgggaggac atgcagggca ggaggtacgt
     8821 gttgcacctg gagacagggg agatatcagt aatatatgaa tatattcggt tcattgaagg
     8881 tagagatggt tctgaacttg atcagaatgg tatctattat attcctggtg tatgccaggg
     8941 aatcaagatc atgaaaaaag gtgtagccgc attccctgga aatcaggtgt gtaaaagtgg
     9001 aatagaagaa tgatacagta tcatccaccc cgttgatgcg tataagaaat ccgcaggcgg
     9061 atgcggaggg atccagaggt ataagtgcag gctgcacccg gcttgctttt ttataaacag
     9121 cactgtcttt cccgataccg tacattgaaa ggctgtcggg cgggacgggt tttccctggg
     9181 aagtccggta gaatgtggcc ttgaggtaag aatttgtttc ttcaaaacat gacccgggtg
     9241 tacaggaaac agacaagaaa agggcacagg caacaacaac cgcggaaata atattaaaag
     9301 tgtttctcat gactttctgt ctgtaaagcc ataaaagtat aaaaaatctc cgcattccgg
     9361 atttttcagc ttaatcgttc cctgataaac ttaccggctt tttccagtgc ccttgctatt
     9421 ccgccgggat tttttccccc ggcagttgca aggaatggct gaccgccgcc cccgccgctt
     9481 atttccggag caatttcccg gattatttcc gttgcttcaa gcctgccttc ctttgccagc
     9541 ggatcactga ccattgcaac gagtccggcc ttttcaccaa tttgtgatcc cataacggca
     9601 acgtttttgc cgggtatttt acgtatctca aaggcaattg acttaagggt ttctgccgag
     9661 tcagcctcca cctgcccccg gataaacctg acatcaccaa tctgaacggc gttttggaca
     9721 agatccctga taatatcttt tgctatcctt gacttgtatt tttcaactgt ttttctgagt
     9781 gccgcatttt cgcttataag cttttccacg ctttccctga cgtttccggt actttttagc
     9841 attgatgcaa cattctccag ctctgcaatt ttttcattta tgtattcatc agcctttgag
     9901 cctgttattg cttcaatcct tcttattccg gctgcgattg ccccttcgga gattattttg
     9961 atcaggccaa tgccggctgt tgagtcagcg tgtgtcccgc cgcataattc cacagagtca
    10021 ccgaaccgaa ccacccttac cgtatcaccg tatttttcgc cgaacagtgc cattgcgccc
    10081 attgtaagag cttcctccag gggaacgtcg tccatgacct gccttgtgaa gttatttctg
    10141 accagctggt ttgcaatatt ttcaacttca aggatttcct cccttgtcag cctgctgaaa
    10201 tggctgaaat caaatctcag ccgttctgag gtaacgagtg atcctttctg ttcaacgtga
    10261 ttgcccagga ctttcctcag ggcatagtga agaaggtggg ttgcagtatg attgctggca
    10321 gtcattgccc ttttccgcag atcaactttt gccacgaagg ctgcatcggg gcgggaggga
    10381 attttatccg ccagatggat gatgagttcg ttttcccaga tggtgtctgt gattcttatc
    10441 tgctcttctt ccgaggcaat gacgccactg tctccggact gacccccgga ttccgcataa
    10501 aaaggtgttt tgtcgaacac gagctggcag atatcctttc ccctggcctt caccacgcgg
    10561 tatttcatta taaggatgtc atcagttgtt ttgtcatatc ccgtaaatat agttgtatct
    10621 cctcccctta cattgatcca gtcgcccgaa tccagtgtcc cgtcctttct ggaccggttt
    10681 ttctgattct ccatttcctg ttcaaagccc tcagtgtcga gagtcatatt gttctccctg
    10741 agtatgagac gggtcaggtc ggcggggaag ccatatgtgt cataaagttc aaaagctatc
    10801 ctgcccggaa gaacatttat ttttttcatt ttcaggtccg aaatcattct gtcaatcatt
    10861 ttcaggcctt tcccgagtgt tttcaggaat gagctttctt cttcatgaat tattctggat
    10921 atatgttctt cagagttttt cagttcgggg tactgatccc ccatttccgc gacaaggaca
    10981 gggacaagcc tgtacatgaa gggttcgtcc acgttcaggt agttgtatgc atatcttaca
    11041 gccctgcgga ggattctccg gataacatat ccggccttgt tgtttgaggg cagttgtccg
    11101 tctgcaattg aaaatgatat tgccctgagg tgatcggcaa ccactctcat ggcgatatcc
    11161 tgttcttccg tttcgccgta tttccggccg catgagattg atatttcatt gatgagggtc
    11221 tggaaaatat cggtatcgta gtttgatgtc ttgccctgga ctaccataca gagcctctca
    11281 aaccccattc cggtgtccac atggctgtcg ggaagcagtt cgagcgatcc gtcggccctg
    11341 cggttatact ggatgaatac cagattccat atttcaagca ccaggggatg gctccggttt
    11401 acaaggctgt ggccgggaat tttttccctt tcgctgtcgt cacggagatc cacatggatt
    11461 tccgaacagg gaccgcaggg tccggtttcc cccatttccc agaaattgtc ctcctttgat
    11521 ccctcaagta ttctgtcgga ctgccccggg aagcattcct gccaccacct gaaggattct
    11581 tcatcgcggg gcacattgtc ttccgggctt cctgtaaata ttgtggcata gagcctgtcc
    11641 gcgggtattc ccagcctgcc gttcaggaat tcccaggccc attcaatggc ttccttcttg
    11701 aagtaatcac caaaagacca gttgcccagc atctcaaaca tggtatgatg ataggtgtca
    11761 tgaccaactt cctccaggtc attatgcttt ccggaaaccc tcaggcattt ctgtgaatca
    11821 gcaaccctcc ggtattttgg tgcggtatgg ccgaggaaaa tatccttgaa ctgattcatc
    11881 cccgcgttgg taaacatgag agaagggtca ttcctgatta ccatgggagc agaaggtaca
    11941 atctcgtggc ccttttccct gaaaaaatta aggaacaact gtctcagttt ctgtgatttc
    12001 atattttttt atatatctgt gtaacatttc gtcaaagtat tcgtaaattg gtttgtaaaa
    12061 ttaatttaaa tgtttagatt tgaggcttgc ggctacaaaa tattccagaa acagaagtgt
    12121 ggagtatgat taagaagaaa tattatctcg atcctaatga tttgcagtat aaacgattaa
    12181 gacttccctg gaaaaggaag ttcttaatgg gtctgctttg gtttacagta acagttgtcc
    12241 tgactgttat ttattcaaca atctttcaca gtatattcgg ctcacccaaa taccaggcgc
    12301 tcagccgcca gttatccgcc ctcaagctga actttacgct tgcaaaccgg gaactggatc
    12361 ttaccgttga catgatcaat gatctgaaga tgtcggatga agtaagatac cgtcccgttc
    12421 ttgcgatgga gagccttccg gagtcctacc ggaatccggg cttcggaggt attgaccgtt
    12481 tcagggatct gaacggttac ccgagttcat ctatcatgag aaataccagg accaggattg
    12541 aagagatgaa gaatatgctg gaagttcagc aggcgtcgtt caggtcgata attgaaaggg
    12601 aagctgaatg gtcgcggatg tacagttacc tgccgcttat atgtccggtt tcggttaaga
    12661 taaggagggg ggagggagtg atgtacaggg aagtacatcc tgtgctgggc acctctcagt
    12721 ggcatcatgg ccaggatttc agaacccctt atggtacaga ggtatatgct acaggaaacg
    12781 gaagggttat tgaggccgga tggaacagcg gagggttcgg aaattatgta gtaattgatc
    12841 atggttacgg atatcagtca acatatgcac acctgagcgg tattaaagtc agcaagggaa
    12901 tggaggtgaa gcgaggagat ctgatcgggc ttaccgggaa ttccggaata tcaacgggac
    12961 cgcatcttca ttaccagatc gatttatacg gaaggtatca gaatccattg catttcttca
    13021 atgatgatct gacggaagat gagtattttg aaatgatcaa tcttcttaca tcaagtaaaa
    13081 aattctgaat cagatatctg tctgcctgtt gacaaatcct gaatgtgttt aaaagtgcag
    13141 ttttgaattt tcatttattg attatctttg tgctgtggtg ccaaagtcaa aatataaatt
    13201 caatcctgat tccctgagtt tcgataaggt aactctcaga gccaaggata tttttctgag
    13261 gcttatggct ttttttgtcg gatcacttct tattgccgtt ttatattatc tgatatttgc
    13321 ccttttcttc aattcgccca aggagaaagc gcttatccgg gagattgagc agcttgcact
    13381 tcagtacgaa attattcaga gggatatggg caatatggaa aaagtgattg tacagcttca
    13441 ggaaacggac gataatcttt acaggaccat attcgaagcc gaacccattc cggtcacact
    13501 gcgtgaaggc ggtgtcggcg gggtaaacag gtataaggaa cttgagggct tcagcaattc
    13561 aaacatcgtt atcgaaacaa ccaggaagct tgacaagctc aggaagcaga tctatactca
    13621 gtcaaaatcc tttgatgagc ttattatcct ttcc
//
LOCUS       DAOSPX010000050        13649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4588_length_13649_cov_4.593203, whole genome shotgun sequence.
ACCESSION   DAOSPX010000050 DAOSPX010000000
VERSION     DAOSPX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13649
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4588_length_13649_cov_4.593203"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLZ75_04085"
     CDS             <1..317
                     /locus_tag="PLZ75_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOS71611.1"
                     /translation="IAQNIPRSLHVRLIAPFYWRVENVMKKKELDITKAEEYVVDTDE
                     KRHQLMTTFLEKKVVNVDYLFDITINRKSFGVEETADMIVSMYEKKTGHLKAAKPRSK
                     AD"
     gene            complement(420..1673)
                     /locus_tag="PLZ75_04090"
                     /pseudo
     CDS             complement(420..1673)
                     /locus_tag="PLZ75_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            1875..2810
                     /locus_tag="PLZ75_04095"
     CDS             1875..2810
                     /locus_tag="PLZ75_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOS71612.1"
                     /translation="MDRNLAIGADIGGSHISCAAVDIVSLRILRETLVERDVNNKASA
                     DEIISVWAGALSASISKIPREKLRGIGFGMPGPFDYVKGISYIKGVAKYENLYGHNVK
                     EELSKALNLPEDFPVRFMNDVSSFAVGEALVGKAASYSRSMAVTLGTGLGSAFIKNRI
                     PVVDGPEVPKLGCVYHLPYKDNIADDYFSTRWFVRSYNTLTGKEIKGVKELSEMAPHD
                     QIAMNLFQEFGYNLGTFLTPFLITFNAGIIVMGGNISFSYNFFGPRFEKRLKQEGCEC
                     KVALSDLKEDAALIGAAFLLDNRFWTDIQHALPLM"
     gene            2936..4090
                     /locus_tag="PLZ75_04100"
     CDS             2936..4090
                     /locus_tag="PLZ75_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS71613.1"
                     /translation="MNTQKINLAKIAPVLMACFVMSFVDLVGIIVDRVSSDMHLSASV
                     AQLIPSAIFLWFFLLSVPIGVLQARIGKRNMLNIGMGITALGLLVPWVFYSFAMVMVG
                     AALLGIGNTIVQVSANPLLVDVVPGNRRSSFLSFSQFIKAVGSMLGAPLAALFAAQFG
                     DWKILFLVFGIVSILSVVWLGNVRIEETRTEGLKVSIGSAFKLLGNNFILLMVLSIFL
                     VVGVDVGFNSNSGQFLMKQFGIAQVAAESGRSVYFLGRMLGTFAGAIMLTKISSSRFF
                     MWTSLLGILFLLAILLIKSSALAWVLVFLIGLAVANIWPLVFSIAVGKHPDRSNEISG
                     LMMMAISGGAIIPFLVGWIGEVTNLSIGMLVLVACMVYLAAVGAYCIKNE"
     gene            4120..5379
                     /locus_tag="PLZ75_04105"
     CDS             4120..5379
                     /locus_tag="PLZ75_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71614.1"
                     /translation="MKIGRRDFLRKTGAATVAFTVVPRFVLGGRGYTAPSDQLTKGII
                     GVGGMGRGHISYEGTRLIAVCDVDRNHMAQAIAQAGGNIAAYHDFRELIARDDIDIVH
                     IATPPHWHGIMSVMAAEAGKDIWCEKPMTRTIGEGKKVVEAVQKHGRMFRLNTWFRFK
                     DTFYGMGTEVKPIKKLVENGVFGWPLKVTVSGITGYDWKFYWSGLHNLKPEPVPPELD
                     YDFWLGPAPYKPYNVERVHSKFRGYWDYDGGGLGDMGQHYLDPVQYFLGKDETGPVSV
                     EIDSPQQHYDAVGSWRRITYTYEDGCQIILDGENRDKDAAYIEGPKGKLYRGFRSDIK
                     DIDKIVASLPDPEPQVTTFTESVISRKKFALNESNGHRSCSMVNLGVIALRLGRNLRF
                     DPVKQRFIDDDGANRLIDQPMRDPWRI"
     gene            5414..8812
                     /locus_tag="PLZ75_04110"
     CDS             5414..8812
                     /locus_tag="PLZ75_04110"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS71615.1"
                     /translation="MKIKNIFLICSSFLLISVLSYSQDRRTLETKVADLLAQFPATSL
                     SYTDRLMEDMMTLGEAGLKQICDQIIPAGTGDDTRARFAVDTYSRFLSSAGRESEREL
                     WEKICISYASRKAEPDVTDFFIKQLQLIGSEQAAEALKIYIADKINCTSAISAITAIG
                     GRVAEQILAESLKDWDLPCAAAVMNALASMRSKAAVNDIIFWSGELNTNIKASAYNAL
                     AMGGTREAYSVLLKAAKSHEYRWERTGATAALINYANTLGLNGDIKTSNKITRMLIKK
                     CNDRLTYQNKAAALKTYVTFNGIDAFPELQKAAGHPDVKYRNAAIGFTGLIEGGEVVN
                     KWISHFPRAGQAARPEIIRMLGKRGDEQALGLINSSLSDADVNVRTAAAEALAGMTGS
                     KAVPALIAYMMQNDSPADQEAAVSALKSVSGDREIPALKDVLAKGSPAAKISAMELMA
                     WNRSSENFREILPFAASDDRNVRAAAFRALPGLAGEQDQEALISLLKSEENSGYVAEL
                     QQALAAAANQAADPEKRAAQILKAMDGRMDKKLLIPVLARTGGREALAAVLKEFEAGT
                     PEMREVCFGALTSWTDYSASSALYEICASGNKTYEAPAFAGYVRQIRNANIKPEQKLL
                     LFRKIMPYALSAERKNQILNELGRVKTWQSLFFVAGYLDDPSTSATAARSVMYIALPS
                     ADEATGMYGTNVREILVKAMSKISGQESEYDREMISKYLESMPPDEGFVSMFNGRDLT
                     GWQGLVENPVSRARMKPAELARKQAEADRKVSENWSVKDGCIWFSGRGDNLCSVKLYA
                     DFEMLVDWKITKGGDSGIYLRGSPQVQIWDTSRVAVGAQVGSGGLYNNQIHPSKPLKV
                     ADNQVGEWNTFRIIMIGEKVSVWLNGELVVDNVTMENYWDRSIPIFPTGPIELQAHGT
                     DLAFRDIYVREISDKEYNLTPEEKAEGFVSLFNGRNLDSWTGNKESYVAEDGMIVIRP
                     ERGSGGNLFTNEEYSDFIFRFEFLLTPGANNGLGIRAPLEGDAAYAGMELQILDDSAP
                     IYANLEPYQYHGSVYGVIPARRGYLKPVGEWNYEEVTVKGTSIRIVLNGTVIVDGDIA
                     GPRDKGTMDNRDHPGLKRKSGHIGFLGHGSVVNFRNIRIRDLSE"
     gene            8944..10389
                     /locus_tag="PLZ75_04115"
     CDS             8944..10389
                     /locus_tag="PLZ75_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS71616.1"
                     /translation="MRKLFIPAIILISLAACRHAGPPEPYGPLPSERQLAWHEMEYYM
                     FVHFTVNTFTGKEWGYGDEGESVFNPSEMDCRQWARVAKDAGMKGIIITAKHHDGFCL
                     WPSDYTEHSVKNSVWRNGKGDVLRDLREACDEFGLKMGVYLSPWDRNIAVYGTPEYLE
                     YYRNQLHELLTSYGDIFEVWFDGANGGSGYYGGARETRRIDNKTYYDWPKTHELVRKL
                     QPDAVMFSDAGPDVRWVGNESGMGSLTNWCLLRRDELWPGGDFARILGEGHEDGNYWV
                     PAEVDVSIRPGWFYHPEEDSRVRSPENLLELYYSSVGRNSNLLLNIPPDKRGLLHEKD
                     VESLLAFRKIREREFENELAGGKAARASSVRGRDFKAGNVTDGNPDTYWTVKDGQTAA
                     DITIDLGQETEVNRILIQEYIRLGQRVREYKVEALSDGEWIPLTEGTTIGYKVIRKFP
                     TVRTSRVRLTISKSGACPVISNIELYRAPGE"
     gene            complement(10537..11514)
                     /locus_tag="PLZ75_04120"
     CDS             complement(10537..11514)
                     /locus_tag="PLZ75_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOS71617.1"
                     /translation="MAELLLGLDIGGTKCTVIHGKTGDGGLDIIDRFTFPTETHLGLE
                     HSLGTIFSHIDLMIGKHGLSSGKVKALGVSCGGPLDSKTGIVMSPPNLPGWDNVPLVK
                     LLTDRYGIKSGIQNDANACALAEWKSGAGRGSENMIFMTFGTGLGAGLILNGRLYSGT
                     NDMAGEIGHIRMCSAGPVGYGKAGSWEGFCSGSGIAQLARTMVMEKWQMGERVSFCKT
                     RDDFDNLNAQIVAEAAIRGDETALGVYRTSGEYLGRGLSILIDILNPEVIVIGSIFAR
                     SGKLLEPHTLRIIEAEALALSRNVCRIVPAQLGEDLGDYAALSVAANLL"
     gene            complement(11562..11714)
                     /locus_tag="PLZ75_04125"
     CDS             complement(11562..11714)
                     /locus_tag="PLZ75_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71618.1"
                     /translation="MRGKSETVREKRAGRQPESGRKLRNGLIDSMKEEGNCETGKTAM
                     KEKYRI"
     gene            11768..13006
                     /locus_tag="PLZ75_04130"
     CDS             11768..13006
                     /locus_tag="PLZ75_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOS71619.1"
                     /translation="MALNYIWIAFFLIGFLAALGQLIFAGNTQIFNEIINSIFDSAKT
                     GFEISLGLTGVLALWMGLMKVGERGGVVSILGRLFGPLFERLFPGLPKGHPAYGSIML
                     NFSANMLGLDNAATPMGLKAMKEMQDVNPDKESASNAQIMFLVLNASGLTIIPVTIIV
                     YRTQLGAANPADIFIPIMIATFAATLAGLISVSIVQKINLLDRVVIAYLGVLTAVVAG
                     IIIFFMNLPKEKITSISTFAANFILLLIIVLFIVLAMLRKVNVYDAFIEGAKDGFGIA
                     VRIIPFLVAILVGIGIFRASGVMDFIVKGMAGFFAWLGLDTEFTGALPVALMKPLSGS
                     GSRGLMIDAMKTFGADSFVGRMSCALQGSTDTTFYILAVYFGSVGIKNTRHAVGCALI
                     ADITGAVAAILVSYLFFGPG"
     gene            13039..>13649
                     /locus_tag="PLZ75_04135"
     CDS             13039..>13649
                     /locus_tag="PLZ75_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71620.1"
                     /translation="MSVLAVSILVLISIIAIIILTSTLKLNAFVSLFLVSVFLAFATL
                     PGDTIVTSIRQGFGNTMASIGLLIIFGAMIGVILDRTGATLSIAGYMLSVTGEKNSPW
                     ALGITGFITGLPIFCDSGFIVLSGLTKSFSARSGMALPFMATVLATSLYSVHCLIPPH
                     PGALAAAGIIDANIGNLIIAGILFAIPGALAAGAWAGFMVKGK"
BASE COUNT         3613 a         3047 c         3489 g         3500 t
ORIGIN      
        1 ccattgccca gaacattcca aggtcacttc atgtgcggct aatagcaccc ttttactgga
       61 gagttgagaa tgtgatgaaa aagaaagagc tggatatcac aaaagctgag gagtatgtgg
      121 ttgatacaga cgagaaaaga catcagctca tgactacctt ccttgagaaa aaagtggtga
      181 atgttgatta cctgtttgat atcacaatca accggaaatc attcggtgtg gaagaaacgg
      241 ctgatatgat agtttcaatg tatgagaaaa aaacagggca tctgaaagcc gcaaaaccaa
      301 gatcaaaagc ggattaaccg ccgtgccccg gacggatgcc tggtctgatt attcatcccc
      361 gccgtttata cacagctgat atccccgatt tttttgacat gttcgcttgt ccgcctgtca
      421 agatgctttc tgtaggcatc ccagtcgctt acaggaaatt ttgccacacc gctttcaatg
      481 gctgccctgg ctacagccgg agccacacat gaaataagcc tgggatcaag aggtttcggg
      541 atgatatatt cttttccgaa agaaagttca tcaagattat aggctttgag aacggtttca
      601 ggaaccggtt cctttaccag tgcagccagt gcttttgttg cagcaatttt catttcttca
      661 tttatggctg atgctcttac atcaagtgcc cccctgaaaa tatagggaaa tcccaggacg
      721 ttgtttatct gattgggata atccgagcgg cctgtggcaa atatgagatc atccctcgtt
      781 gcaaaggcat cttcgcggga aatttccgga tcgggatttg ccattgcaaa cactattggc
      841 tggtccgcca tgcttgagag catctcccct gtcagcatat ttgccaccga aagtccgaga
      901 aagagatcgg cacccctgac tgcctcagtc agagtgtcaa tatttctgtc gctgataaat
      961 tcctgtttga atttgttcag atcctttctt ttccggttga tgacaccctt gctgtcgacc
     1021 atgacgatat tttccttttt cacgcccagc atgatataga gacgggtgca ggatattgca
     1081 gccgctcctg ctccgcatac cacaactttt atcttgtctg cctcacggcc ggttaattcc
     1141 agggaattaa gcagagctgc ggcagatata attgccgttc catgctgatc gtcatgaaaa
     1201 accggaatgt caagcatttt cttcagattc tcctcgagtt cgaagcattc gggggccttt
     1261 acatcctcaa gattaatgcc tccgaaagtg ggagaaatct ttgcaattat cccggcaagc
     1321 ttcccggggt ctttttcatc aatttcaata tcgaatacat caacatcggc aaatactttg
     1381 aacagcagac ccttaccctc cattaccggt ttgccggcaa gacagccgat atcgcccagt
     1441 ccgagaactg ccgttccgtt tgaaatgact gcaacaagat tgccttttgc agtatacatg
     1501 taggcgtcat caggattctt ttctatctcc agacatggcc aggccactcc cggtgtatag
     1561 gcaaggctca ggtcaaactg tgtgcaatat ggcttggtag gtatcacttc caccttaccg
     1621 tgcctgcccc tgctgtgata gataagggca tccttcttgg ttatttttaa catggcagta
     1681 tttttttcgc ccggtgatta aaatctgcat cagggaaaca gctattcgaa aatataataa
     1741 ttaaaccgga ataatcatta aaaagatcat gtaataataa caaaatggaa ataaattttg
     1801 tagtgagcac gattttagat attttcaaag cctgtttaaa gcgataataa ttaacttttt
     1861 taatccgcat atttatggac cgcaacctgg caatcggtgc tgatattgga ggaagccata
     1921 tcagttgcgc cgcagtcgac attgtatcgt taagaatatt aagagaaacc cttgtcgaaa
     1981 gggatgtcaa caacaaggct tcggcagatg agatcatatc ggtatgggca ggcgcccttt
     2041 ccgcatcaat cagcaaaatt ccccgcgaaa agctcagagg cataggattc gggatgcccg
     2101 gcccctttga ctacgtgaaa ggcatcagct acataaaagg tgttgccaag tatgagaacc
     2161 tgtatgggca caatgttaag gaggagctgt cgaaagcact gaatctgccg gaagatttcc
     2221 ccgtaagatt catgaatgac gtttcatctt tcgccgtggg cgaagccctg gtggggaaag
     2281 ccgcatccta cagccggtca atggctgtta cactgggaac aggacttggt tcagcattta
     2341 taaaaaaccg gattcctgtt gttgacggcc ccgaggtgcc taaactgggc tgcgtttatc
     2401 accttcccta caaggacaat atagcggatg attatttttc gaccagatgg tttgtcagga
     2461 gctataatac attgactggt aaagaaataa aaggggtaaa ggaactttct gaaatggcgc
     2521 cacatgatca gatagcaatg aatctttttc aggaattcgg atataacctg ggaacattcc
     2581 tgactccctt tctcatcaca ttcaatgccg gaattattgt tatgggtgga aatatttcct
     2641 tttcatacaa ctttttcggt ccgaggttcg agaaaagact taagcaggag ggttgtgaat
     2701 gcaaggtggc actgtcggac ctgaaagaag atgcggccct tatcggcgca gcttttcttc
     2761 tggataaccg tttctggacg gatatccagc atgctcttcc gctgatgtaa aaccgaaatt
     2821 cagaaccgga atccggaact ggtatttaat agaaaactca aagaaagcat aaaagaaaac
     2881 cgcaaaaccc aaacaaacgc gtactaataa acgcgttatc ataaaaaccc tgataatgaa
     2941 tacacaaaaa atcaatctgg caaaaatagc tcctgttctc atggcctgtt ttgtcatgag
     3001 ttttgtcgac ctggtcggaa taattgtcga ccgtgtcagc agtgatatgc atctcagtgc
     3061 aagcgtggca cagctgatac cctcagccat attcctgtgg ttctttctgt tgtcagttcc
     3121 cataggagtg cttcaggcaa gaataggaaa acgcaatatg ctgaatatcg gaatgggtat
     3181 aaccgcactc ggattactgg ttccctgggt tttttactca tttgcaatgg tgatggttgg
     3241 tgcagccttg ctgggcatag gaaataccat cgtccaggta tcggcaaatc cgctgctggt
     3301 cgatgtggtt cccggaaaca gaaggtcgag tttcctgagc ttctcccagt tcataaaggc
     3361 agtgggatca atgctgggcg ctccgcttgc cgcacttttt gccgcacagt tcggcgactg
     3421 gaaaatactt ttcctggtgt ttggtattgt ttccatcctg tctgtagtct ggctaggaaa
     3481 tgtcagaatt gaagaaaccc ggacagaggg cctgaaagtg tcaatagggt cggctttcaa
     3541 gcttctgggg aataatttca tactcctgat ggtgctttca atattcctgg tagtgggtgt
     3601 ggatgtcgga ttcaactcca attccggaca gtttctgatg aaacagttcg gaatagcgca
     3661 ggtggccgcg gaatcgggca ggagcgtcta ttttctcgga cgtatgctcg gcacttttgc
     3721 cggagccatc atgcttacaa aaatttcttc atcaagattt ttcatgtgga cttcccttct
     3781 gggaattctt ttcctgctgg ccattcttct cattaaatca tcagccctcg cctgggtact
     3841 ggtttttctg ataggactgg ctgtcgcaaa catatggccc ctggtttttt ccatcgccgt
     3901 cgggaaacat cctgatagga gcaacgagat ttccggactt atgatgatgg ctatctccgg
     3961 aggagcgatc attcctttcc tggttggctg gataggggag gtcaccaatc tcagcatcgg
     4021 tatgcttgtg cttgtcgcat gtatggttta ccttgctgcc gtcggggcat attgtattaa
     4081 aaatgaatag aaccaacaat cactaatgtt atgaaaaata ttaaaattgg cagacgtgat
     4141 tttctgagaa aaaccggtgc tgccacggtt gcttttactg ttgtacccag gtttgtgctc
     4201 gggggcaggg gatatactgc tccaagcgat cagcttacaa agggcataat aggagtggga
     4261 ggaatgggac gcggccatat cagttatgaa ggtacccgtt tgattgcagt atgcgatgtt
     4321 gacaggaatc atatggcaca ggccattgcc caggccggtg gcaatatagc cgcttatcat
     4381 gatttccggg aactgattgc aagggatgac atagatattg tccatattgc gacaccgccc
     4441 cactggcatg gcataatgtc ggttatggca gcggaagccg gcaaggacat ttggtgcgag
     4501 aagcccatga caagaaccat tggtgaaggg aaaaaagttg ttgaggccgt tcagaaacac
     4561 ggtagaatgt ttcgtctgaa tacatggttc aggttcaagg atactttcta tggtatgggt
     4621 acggaagtta agcccatcaa aaaacttgtt gaaaacggtg tcttcggatg gcccctgaaa
     4681 gtaaccgtaa gcggaataac cggatatgac tggaaatttt actggagcgg attgcataac
     4741 ctgaaaccgg aacccgtgcc gcctgaactg gattatgatt tctggctggg accggccccg
     4801 tataaaccat ataacgttga aagggtccat tcaaaattcc gcggatactg ggattatgac
     4861 ggtggtggcc tgggggacat gggccagcat tatcttgatc ccgtgcagta tttcctcggc
     4921 aaggatgaaa ccggaccggt atcggttgaa atagattcac cccagcagca ttatgatgcc
     4981 gtgggatcat ggagaaggat aacctacacc tacgaggacg gatgccagat aatccttgac
     5041 ggtgaaaaca gggacaagga tgcggcatat attgaaggtc ccaaaggaaa actgtacagg
     5101 ggattcagat cagatataaa ggatattgac aaaattgtgg cttcactgcc cgatcccgaa
     5161 ccgcaggtca caacattcac ggaatcggtg ataagcagaa agaaattcgc actcaatgaa
     5221 tccaacggcc acaggtcatg ttccatggtg aaccttggag tgattgcact cagactggga
     5281 aggaatctca ggtttgaccc tgtaaagcag cgttttattg atgatgatgg agccaacagg
     5341 ctgattgacc agccaatgag ggatccctgg agaatatgaa cgatacaacc ttaaaataca
     5401 aaatgcgaaa atcatgaaga tcaaaaatat atttctaatc tgttcctcat ttctgctgat
     5461 atcggttttg tcatattcac aggacaggag gacgcttgag acgaaagtcg ctgatctgct
     5521 ggctcagttc cctgcaacca gcctttccta taccgacagg cttatggaag acatgatgac
     5581 tctgggagag gccggtctca aacagatttg tgaccagatc atacccgccg gtacaggaga
     5641 tgataccagg gccaggtttg ctgtcgacac ctattccaga tttctttcgt cggcaggaag
     5701 ggaaagcgaa agagaacttt gggaaaaaat ctgtatttca tatgcttcca ggaaagctga
     5761 acccgatgtc acggactttt tcataaagca gcttcagctc attggcagtg agcaggcagc
     5821 tgaagcactt aaaatctata ttgccgacaa aattaactgt acttccgcaa tatcggctat
     5881 tactgccatc ggcggacggg ttgccgaaca gatactggca gaatcgctga aagactggga
     5941 tcttccctgt gctgcagcgg tcatgaatgc tcttgcatcc atgagatcga aggcagccgt
     6001 taatgatatt attttctggt caggcgagct gaatacgaat attaaggctt ctgcatacaa
     6061 tgcacttgca atgggcggga cccgtgaggc atattccgtt ctccttaaag cggcaaagag
     6121 ccatgaatac cgctgggagc gaaccggtgc caccgctgca ctgatcaact atgccaatac
     6181 cctgggactc aatggtgata tcaaaacctc taacaagata accaggatgc tcatcaaaaa
     6241 atgcaacgac aggcttacct atcagaataa ggctgcagca ctgaaaacct atgtcacttt
     6301 caatggaatc gatgcatttc ccgaactgca gaaggcagcc ggacatcccg atgtgaaata
     6361 caggaatgca gccatcggtt ttaccggatt gatagaagga ggtgaggtcg tgaacaaatg
     6421 gatcagtcat tttcccaggg caggtcaggc ggccagaccg gaaataatca gaatgcttgg
     6481 aaaacgcggg gatgaacagg cgctcggtct tatcaattca tccctgtcgg atgctgacgt
     6541 taatgtaagg actgcggcag cggaggcgct tgcagggatg accggcagca aagccgttcc
     6601 ggcacttata gcctatatga tgcagaatga ttctccagcg gatcaggagg cagcagtgtc
     6661 agccctgaaa agtgtttcgg gcgacaggga aatacctgca ctgaaggatg ttctggccaa
     6721 aggctcgccg gcagcaaaaa taagtgcaat ggaactgatg gcctggaaca ggagcagtga
     6781 aaatttcagg gagatactgc ctttcgcagc atccgatgac agaaatgtga gggccgctgc
     6841 attcagggcc ctgcccggac tggctgggga gcaggaccag gaagccctga tcagtctttt
     6901 gaaatcggaa gaaaattccg gctatgttgc ggaattgcaa caggcacttg ctgcagctgc
     6961 aaatcaggca gcagatcctg aaaagagagc ggcacagata ctgaaagcca tggatggcag
     7021 aatggataaa aagcttctta ttcccgtact tgccagaacc gggggaagag aggcgcttgc
     7081 agccgttctg aaggaatttg aagccggtac tcccgaaatg cgggaagtat gcttcggggc
     7141 acttacttcc tggaccgatt attccgcatc ctcggcatta tacgaaatat gcgcatcagg
     7201 taataaaact tacgaagccc ctgcctttgc agggtatgtc aggcagatca gaaatgcaaa
     7261 catcaaacct gaacaaaagc ttctgctttt cagaaagata atgccatatg ccctgtcagc
     7321 cgaacggaag aaccagattc tgaatgaact cggcagggta aaaacatggc agtcactctt
     7381 ctttgtggca gggtatcttg atgatccgtc aacatccgcc acggcagcaa ggtcggtaat
     7441 gtatatcgca ctgccttcag cagacgaggc aacagggatg tacggtacaa atgtgaggga
     7501 gatacttgtg aaggcaatgt cgaaaatatc cgggcaggaa agtgagtacg acagggagat
     7561 gatcagcaaa taccttgaat caatgcctcc ggatgaagga tttgtttcaa tgttcaacgg
     7621 cagggatctt acaggctggc agggactggt tgaaaacccg gtgagccgtg caaggatgaa
     7681 acctgcggaa cttgcacgga aacaggctga ggctgacagg aaggtttcag aaaactggag
     7741 cgtgaaagac ggatgcatct ggttcagcgg gcggggggat aatctctgtt cggtgaagct
     7801 ctatgccgac tttgaaatgc ttgtcgactg gaagataaca aagggaggag acagcggaat
     7861 atatctcagg ggttcacccc aggtacagat ctgggatacc tcaagagttg cagtcggggc
     7921 ccaggtagga tctggcggat tgtacaacaa tcagatccat ccctcaaagc ccctgaaagt
     7981 agccgataat caggttggcg agtggaatac attcagaatc attatgatcg gagaaaaggt
     8041 gtcagtgtgg ctcaacggtg aacttgtggt ggacaatgtt acgatggaaa attactggga
     8101 ccgcagtata ccgatctttc cgaccggacc gatcgagctt caggctcacg gaaccgatct
     8161 ggcattccgc gatatttatg tcagggagat aagtgataag gaatataatc ttacaccgga
     8221 ggagaaagca gaaggatttg tttccctgtt taacggaaga aatcttgata gctggacagg
     8281 caacaaggaa tcatacgttg cggaagatgg catgatagtg atcaggccgg aaaggggcag
     8341 cggggggaat ctgtttacaa atgaagaata cagtgatttc atattccgtt tcgaattcct
     8401 tctcacaccc ggggctaaca acggtctcgg tataagggcg cctcttgaag gagatgcagc
     8461 ttatgcagga atggaacttc agatccttga tgacagtgcg ccgatctatg caaacctgga
     8521 gccttaccag taccacggat cggtttacgg ggttatcccg gccaggagag gttacctgaa
     8581 accggtcggt gaatggaatt atgaggaagt tactgtgaag ggaacatcca tcaggatagt
     8641 tctgaacgga acagtcattg ttgacggaga catagccgga ccccgcgata aaggaaccat
     8701 ggacaacagg gatcatcccg gactgaaaag gaagtcaggg catataggct ttctgggaca
     8761 tggttcggtt gttaatttcc gcaacatcag aatcagggac ttgtcagaat aaagaacttt
     8821 ccggactaat attctggatt ttaaaaacag attgaaaata aacggataag aacacaagga
     8881 tcattatccc tgcattattg ttttcataag cctttcatga cactttcatt aaactttatt
     8941 aaaatgagaa agttatttat tcccgcaatt atcctgatca gcctggcagc ctgcaggcat
     9001 gccggaccac cggaacctta cggtcctctg ccttcggagc gtcagttggc ctggcatgag
     9061 atggagtatt atatgttcgt tcatttcact gtcaatacct ttacaggcaa ggaatgggga
     9121 tacggagatg aaggagaatc ggttttcaat ccttctgaaa tggattgcag gcagtgggcc
     9181 agggtggcta aagatgccgg aatgaaggga atcataataa ccgcgaagca tcatgacggc
     9241 ttttgtctgt ggccgtcgga ttatactgaa cattccgtga aaaattcagt atggagaaac
     9301 ggaaagggtg atgttttaag ggatctcagg gaggcatgcg atgaattcgg actgaagatg
     9361 ggagtgtatc tttctccgtg ggacaggaac attgctgttt acggaacgcc ggaatacctt
     9421 gaatattacc gtaatcagct tcatgagctg cttacatcct acggcgacat tttcgaagta
     9481 tggtttgacg gggccaacgg aggcagcggt tattacgggg gtgcacgcga aacacggcgg
     9541 attgacaata aaacctatta cgactggccg aaaacgcatg aactggtaag aaagctgcag
     9601 cctgacgcgg tgatgttcag tgatgccggg ccggatgtca gatgggttgg caatgaaagc
     9661 ggaatgggca gtctgacgaa ctggtgcctc ctgcgccgcg acgaactgtg gcccggcggg
     9721 gattttgcaa gaatcctcgg agaaggccat gaagatggca attactgggt tcccgcggag
     9781 gtggatgtct caatacgccc cggatggttc tatcatccgg aagaggattc ccgggtccgt
     9841 tcaccggaaa accttctgga actttactat tcttcagtag ggcggaacag taacctgctg
     9901 ctgaatattc ctccggataa aaggggattg ctgcatgaga aggatgttga atcgcttctg
     9961 gctttcagaa aaataagaga aagggaattt gaaaatgaac tggccggagg caaggctgca
    10021 agggcttctt ctgtcagggg aagggatttt aaggcaggaa atgtgacaga cggaaatccg
    10081 gatacatact ggaccgtgaa ggacgggcag actgcagcag acattaccat tgacctggga
    10141 caggaaacgg aagtgaacag aatacttatc caggaatata taagactcgg acagcgtgtt
    10201 agggagtata aggtcgaggc cctcagcgac ggggaatgga ttccgcttac ggaaggcaca
    10261 acaataggat acaaggtgat caggaaattt cccacagtca gaacttcacg ggtcaggctg
    10321 acaataagca aatcgggggc ctgtccggtg atctccaata tagaacttta cagggctccc
    10381 ggggagtaat tggctcaggg cacagggctc agggggagta attggctcag ggcacaggac
    10441 acaggactca gggttcaggg tgcagggttt cgggtgcagg gttcagggtg cagagggagg
    10501 gactttaagt ggccggtatt aactgatcaa ttattgttac agtaaattcg ctgctacgga
    10561 aagggctgcg tagtcaccaa gatcctcccc gagctgtgcg ggtactatcc ggcatacatt
    10621 cctggaaagg gcaagggctt cggcttcgat gatccttagt gtatggggtt ccaacaattt
    10681 cccggacctt gcaaaaatgc ttccgatcac tatcacttcg ggattcagaa tgtcgataag
    10741 tattgacaga cctctgccga gatattctcc ggaagtacgg tataccccaa gtgccgtctc
    10801 atctcccctg attgcagctt cggccacgat ctgcgcgttc aggttatcaa agtcatccct
    10861 ggttttgcag aagcttactc tttctcccat ctgccatttt tccatgacca tggttctggc
    10921 cagctgggca ataccgcttc cgctgcagaa gccttcccag cttcctgcct tgccgtagcc
    10981 cacgggaccg gcagaacaca tcctgatatg gcctatctct cccgccatat catttgtgcc
    11041 cgaatacagg cggccgttga gaatcagtcc ggccccgagc cctgttccga aggtcatgaa
    11101 tatcatgttt tcgcttcctc gtccggcccc ggatttccat tcggcaagag cacaggcatt
    11161 tgcatcattc tgaatgccgg atttaatgcc gtatctgtca gtcagcagct ttaccagcgg
    11221 cacattgtcc catcccggca gattcggcgg tgacatcaca atgcctgttt tactgtcaag
    11281 aggcccgcca cagcttaccc caagggcttt cacttttccg gatgaaaggc cgtgctttcc
    11341 tatcatcagg tcaatgtgac tgaatatggt gccgagggag tgctcaagtc ccaggtgggt
    11401 ttcagtgggg aaagtaaacc ggtcaattat gtcgaggccc ccgtcgcccg ttttgccatg
    11461 gataacggta cattttgtgc ctcctatatc caggcctaat agtagttcag ccatgtttca
    11521 tatgttaatc cgttcatact gactaaaagt aataatttaa ttcatattct gtatttctct
    11581 ttcattgccg ttttgccggt ttcacagtta ccttcttcct tcatgctgtc aattaagccg
    11641 tttcgcagtt tccttccgct ttccggctgt ctccctgccc gtttctccct caccgtttca
    11701 cttttccctc tcacctatta aatatatctt ccataatcct tatctttagc accacataga
    11761 ctcctttatg gctttaaact atatctggat agcttttttt ctgatcggat ttctggcagc
    11821 tctcgggcaa ctgatttttg caggcaatac acagatattc aacgaaatca ttaattccat
    11881 ctttgacagc gcaaaaaccg gatttgagat atctctggga cttacgggtg tgcttgcctt
    11941 gtggatggga ctgatgaagg ttggtgaaag gggaggagtg gtttcaatac tgggcaggct
    12001 cttcggtccc ctttttgaga gattgtttcc cggtttgccg aagggacacc cggcctacgg
    12061 ctccatcatg cttaatttct cagccaacat gcttggccta gacaatgccg ctactccgat
    12121 gggactgaag gctatgaagg agatgcagga tgtaaacccg gataaggaaa gcgcctcaaa
    12181 tgcccagata atgtttctgg tactgaatgc atcgggactg accattattc ccgtaacgat
    12241 aatagtgtac cggacacagc ttggtgcggc aaacccggcg gatattttca ttccgataat
    12301 gatcgcaaca tttgctgcga cattggccgg actcatcagt gtttccatcg tccagaaaat
    12361 aaatctgctt gacagggtgg taatcgcata tctcggggtt cttacagctg ttgtggccgg
    12421 aataataatc tttttcatga atcttccgaa ggagaagata acttcaattt caacatttgc
    12481 cgcaaatttc attctgcttc tgatcatagt actgttcatt gtcctggcga tgcttagaaa
    12541 ggtgaatgtg tacgatgcat tcatcgaggg agcaaaggac ggattcggaa tagcggtcag
    12601 gattataccc tttcttgtgg caatactggt tggtatagga atattcaggg catcgggtgt
    12661 aatggatttc attgtaaagg ggatggcagg attctttgca tggctgggac tggataccga
    12721 attcaccggc gctcttccgg ttgctttgat gaagccgctg agcggcagtg gttcaagggg
    12781 gctgatgata gatgcaatga agactttcgg ggccgattca tttgtagggc gtatgtcatg
    12841 tgcattgcag ggctcaaccg ataccacctt ctatatcctc gcggtttatt tcggatcggt
    12901 gggaataaaa aacacaagac atgccgtggg ctgtgcactg atcgccgata taacgggggc
    12961 agtggctgcc atactggtct cttatttgtt tttcggcccg ggctgatttg accgtgatag
    13021 attattccta agctgatcat gagtgtactt gctgtttcca tactggttct gataagcatt
    13081 attgcaatta tcatcctgac ttcaaccctg aagctcaatg cctttgtttc actgttcctg
    13141 gtttcggtat tccttgcctt tgccacactt ccgggcgata cgatagttac ttcgatcaga
    13201 cagggctttg gcaataccat ggcctccatc gggctcctta tcattttcgg agccatgata
    13261 ggtgttattc ttgacaggac gggtgccacg ctgagtatag ccggctatat gctttccgtt
    13321 acgggagaaa agaacagtcc gtgggccctg ggcatcacgg gttttataac cggtctgccc
    13381 attttctgcg attcgggatt cattgtttta agcggactga caaaatcatt cagtgccagg
    13441 tcagggatgg cgcttccctt tatggctacc gttctcgcaa catcgcttta ttcggttcac
    13501 tgtctgatac ctccgcatcc aggggctctt gcggcagccg gaataattga cgcgaatatc
    13561 ggcaatctca taatagcggg aattttattc gccataccgg gtgcccttgc tgcaggagca
    13621 tgggccggat ttatggtcaa agggaagaa
//
LOCUS       DAOSPX010000051        13624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4599_length_13624_cov_4.035964, whole genome shotgun sequence.
ACCESSION   DAOSPX010000051 DAOSPX010000000
VERSION     DAOSPX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13624
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4599_length_13624_cov_4.035964"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLZ75_04140"
     CDS             <1..211
                     /locus_tag="PLZ75_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HOS71621.1"
                     /translation="AGMHANVVRSVMEAGPARIVYISCNPATQARDISMLSERYEVVK
                     IQPVDMFPQTHHVENVALLKLKNET"
     gene            308..1081
                     /locus_tag="PLZ75_04145"
     CDS             308..1081
                     /locus_tag="PLZ75_04145"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOS71622.1"
                     /translation="MYNQSIDEHLASLRYAGIIQQALMPDDEHLASLFRDHFVFFLPR
                     DIVSGDFYYALRYRHYICVAAGDCTGHGVPGALLSIMGISFLNEILQARCEPRANRVL
                     NFMREKVMKALHQTGEKSEAKDSIDIGLCVFDTESQKLQYSGANRPLLRIRDGEVSEF
                     KPDKMSIGLAPVHEHPFTNVIMDTRPGDTFYLYTDGFSDQFGELTDKKFKHNQLKKVI
                     AGVSGFPMSRQREVLEDTFYDWKGNTPQIDDVLVFGFRI"
     gene            1093..3372
                     /locus_tag="PLZ75_04150"
     CDS             1093..3372
                     /locus_tag="PLZ75_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS71623.1"
                     /translation="MMKLIAFRIRNFRSITDTGWQSLSPDNITCLIGQNESGKTSILE
                     ALKVFYTGVISEDVLRSDLSLPEVHCRFRIPEGWLLNITDNPGPELRELLAGLDSLEL
                     TRTWEADLSSVIKVSGAVSQYLDSLEDAWKIYLGDVTLRLEEEMDNIDVLEATLENIS
                     RQESLLSSQLPSRDSAKKPFSFLSRKNGNERAAAGVNPELYSELDDLRDKKARITSEL
                     ESRKGMKQAGEKWKEILDRYSMYEKHMSELFLKLEERHQKLTLLMKPFDDEDIAWKSV
                     LQDYRKTREDRDMIKSELDRHIAFSGYLLNGYSEAEAEEKVGEIIQSYKSQFNSEMLG
                     RKYFAHCPVFEFFEDFSSLLPNRIDLHDIISGNEQVEGYKAARNFLTIARLDYSFFQQ
                     RSSRILKQKIENINQTLTTDFQDFWQQSIGRNNKIHIQFELDHYDATAGRKSGKPYLE
                     FWIKDEGERLYPKQRSRGVRWFLSFYLELKASANINREMVLLVDEPGVSLHARAQEDV
                     LKVFEDIRERIQVVYTTHSPHLVEINKLHRVLAIQRDDIDSMRSSTRILDPLQLSSAT
                     PDTLSPLQSILGNPLPVEGAAPGKFNLIVNDNGTFYLINAILHLTGFKNRISVIPSTG
                     VSSIPLLCNILMGWGLDFLVLLFRNDMEEDIVETLQETVYKPGSNGKETIIMMPENFH
                     NSEDLLSTLDFKNHILKTREGITVSNSDYIREKEMPRNFVFSRFLSEVMDGDIRLSDF
                     DEETNENFRLLIDKLKGHK"
     gene            complement(3567..5621)
                     /locus_tag="PLZ75_04155"
     CDS             complement(3567..5621)
                     /locus_tag="PLZ75_04155"
                     /inference="COORDINATES: protein motif:HMM:NF021534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HOS71624.1"
                     /translation="MKNTFEVKNPDWKLSPFTGMTKKHYIECAKYLLERAFKHVRSIE
                     TPISFPKIPGKTYPQPDAPDWRYRSADFEALERTFTLAGPLIHVDPDTKINNINLRDY
                     YCRHIYNALTPGHPNSLPLPENLPDSTYQFTCEFGGLFKTLLLMPETLWPHFTREQRD
                     EIAVTMSKWAHHRTTQNNWRIFNIVTLSFLKKYGYEIDEELLKSHLLWVASYHSGNGW
                     YLEQTYNYYTISLFIVYGTIWNRAFGDEYYPEIAETIEKSAYELMKTYTDFFARDGYV
                     NMWARSICYRTWISGGFPVSFMLKGISPLDPGWARRLCSGSLLQFVTREEFYENDIPS
                     LGFYGHREYVLQNYSCAGSPFIMFLPFICLALPDDSPFWTAKENEGIWEMLGNNSKTT
                     VLENPGLVLVNHGKTGTSEIIPGKVYYDDHNYSKLVYNTHFPWEDHNPGGGTSMEYSF
                     RSLDPRDVRGDDINFYLTGHTLQNDPEKNAGFTTSQSMLYNGVRKNVLYRQAIMRKPP
                     NNGVGYIIDLAEIIIPGGIIRIDRSRLAFEYELTLGHYGLPHIKGNKAEIHQFEEGTK
                     KVITAAIPGRRLALITYGGWDKVAATIHTGRNAEADESTVLYAYKKRMAKNPAIELMI
                     TVLLHKTDDNEWTEEELSPVKKIEIMDITPSYSVLGANITLSDNKEYEIDFKDIDGYR
                     TC"
     gene            6121..6207
                     /locus_tag="PLZ75_04160"
     tRNA            6121..6207
                     /locus_tag="PLZ75_04160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6155..6157,aa:Ser,seq:gct)
     gene            6609..7592
                     /locus_tag="PLZ75_04165"
     CDS             6609..7592
                     /locus_tag="PLZ75_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOS71625.1"
                     /translation="MKPVLYLVLFSLFIIFSCREAERIPAIIFDTDLGPDYDDAGAMA
                     VLHAMADSGKAEILATLSSNRHELVAPSIDVINTYFNRPDLPVGAPKTGGISMGSGQH
                     WADSIVSVYPHRIRSTEEVPDAVEVYRRILSDRPDRSVTIVTVGFLTNLSNLLMSQPD
                     VYSPLNGRELVERKVKRLVSMAGSFPEGREFNVYTDSVASKHALDNWPGEIIFTGFEI
                     GSRILTGLKLIAAGIKDNPVRDVYRISIPLSAGDKYGRMSWDHTAVLIAVYGTGGFFD
                     TVRGRIIVNSDGSNVWENDAEGPHSYVVQKMPADEVAAFIESRMMHVPERK"
     gene            complement(7552..8274)
                     /locus_tag="PLZ75_04170"
     CDS             complement(7552..8274)
                     /locus_tag="PLZ75_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS71626.1"
                     /translation="MEYYLRKLIEEGENQYVDFKYCVSDSRKIARTLSAFSNTDGGRL
                     LIGVRDNGSIAGIQSEEEIFMVDTAAQIFCRPEINYTIKQHAVGVKTILEVEVSKGTK
                     RPYQAQDENGKWLTYFRHNDQNLLANRVLLQVWRKEGKKTGVMVKFSKAENILMDYLA
                     ENGSISISKFKKIAKISSCRAESILANLLLFKVLVMKASERGFSYEINPEDPMKLLNE
                     KTYPATHSIFISFQEHASSSTR"
     gene            complement(8336..9613)
                     /locus_tag="PLZ75_04175"
     CDS             complement(8336..9613)
                     /locus_tag="PLZ75_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOS71627.1"
                     /translation="MARFTGIIGIIVILGLAFLWSNNRKAINYRLVFTGLGLQIALAI
                     FILKVPIGQEIFHWLGKAINKLLDFSQEGGSFVFGDLTKVQEILPPTIVSGGVFLFIL
                     IPTIIFVCVLVAMAYHIGLMQRVVALIARFVHWVMQVSGSEALSNVASAFVGQVEAQI
                     MIRPYLSGMTMSELLASMTGSMACIAGGVMAVYISMGVPASYLLAASLMAAPGALVIS
                     KIVYPETKVSETKGKVTVEVKKEHANIMDAISHGASDGWKISTNVIAMLIGVIALVAL
                     ADAILGYLGQLLSLLGASLDFIGLDIENLRLKDVFGLVFSLFAIIMGVPVAESFEVGS
                     LMGTKMVINEFVAYTGLAPMIAANSLSPKAIVIASFALCGFANFSSIAIQIGGIGAMA
                     PTRKADLARLGFKAMLCGTMASYISASLAGILL"
     gene            complement(9752..10546)
                     /locus_tag="PLZ75_04180"
     CDS             complement(9752..10546)
                     /locus_tag="PLZ75_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71628.1"
                     /translation="MKQELQSPPAGKKLHIFILTVAVIFMIIHLVLSMSAPEKKLNEL
                     RTAYGPDKNIKNPPDERILADPTYLALMKEKAYYQSRVTMAASDSIYMTVNLADSTVN
                     LEIGGVVVHTARILRKRISSILQRDEYAILNMLSSPFTIASDVSTIRKEPLMIKMAPR
                     DTSEYQPDMIPDTTDFEPVNFILLMENGLKLHVYQDEKLKKGDNFHRFLFDLRDRLET
                     TAASLSRTITFRVPEYYPAIRIRLARGDVKRIYRALPYRGQIGIYR"
     gene            complement(10557..11357)
                     /locus_tag="PLZ75_04185"
     CDS             complement(10557..11357)
                     /locus_tag="PLZ75_04185"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOS71629.1"
                     /translation="MTDPKKIRFLEADRIRKTALNILSKLKSLVIQFFEKIRENEKLG
                     AVLLKIYMSAIFSAAIAFLIIFLLWIVPLAQELCCGRVASPPAASEVKEDPSYQKTIA
                     RIRTDTERLERRYNSYTTGQSYLVINTTDNIFHLYRNKKEIRTGPCSSGSYKMLQTHE
                     GRSWIFKTPKGRFRIRAKTVNPVWTRPDWAFVEEGLPIPAHNHPSRYEHGVLGKYSLS
                     LGDGYLIHGTIYKRLIGMPVTHGCVRLGDEDLEVIFRTLNIGSKVYIF"
     gene            complement(11368..12480)
                     /locus_tag="PLZ75_04190"
     CDS             complement(11368..12480)
                     /locus_tag="PLZ75_04190"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOS71630.1"
                     /translation="MRKRKRNLLIFLLTMTALLSAGFLFIFFLPKPPAGEMIFAVESL
                     SVAKRNQADRYTPEFYNQASVSCDSGMAIWRRENRKFILFRKFDLVREYALLSAGMSD
                     SANIRTALLKAEMKEGQARIIDTLNKIVKEINDHYSSFPYSFEIRERITRGKMLLIDA
                     ETAFTEGNYIDADSYITDSENLLVSSREFADLNLREYFQSFPQWEKWIALTLSESEKT
                     GGYAIIVDKFARKLILYHGGKKIREYSAELGRNWIGSKRIMGDMATPEGMYQIIKKLE
                     GSGTKYHRALLLNYPNSEDMARFKRDIRNGALPPTADIGGMIEIHGGGGRGADWTEGC
                     IALANNDIDALFPYVRVGTRVTIVGSLYDQKKIFEN"
     gene            complement(12692..13288)
                     /locus_tag="PLZ75_04195"
     CDS             complement(12692..13288)
                     /locus_tag="PLZ75_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71631.1"
                     /translation="MKNKMLMGFAVIVMIGILAGCGKVPQAQIDATNAAIEAARTVQA
                     DIYLPDEFAAIQDSMNAILVNIELQKSKLFKNFGDEKDRLDQVTNMANQTAARVDAKK
                     EEVKQEVQNLLNDTRNVVAENSRLVTRLPRGKEGAQVIEQIKADLSGVENSVSEAQNL
                     FDKGAFMDALNRVKAARAKADGLNSEMKEVLTKARIRF"
BASE COUNT         3542 a         2945 c         3121 g         4016 t
ORIGIN      
        1 agcgggaatg catgcgaatg ttgtcagatc agtgatggag gccggacctg ccaggattgt
       61 ctatatcagc tgtaatccgg caacccaggc cagggatatt tccatgctgt cggaaaggta
      121 tgaagtggtg aaaattcagc ctgttgatat gtttccgcag acacatcatg ttgaaaatgt
      181 agctcttctg aaacttaaga atgaaacata gataacaata gaaccaaaaa tattgtatat
      241 ttgtctatct gataggtgaa agtaaatgtc gtctgaaact caatttacta atgagatcat
      301 atctctgatg tacaatcaga gtattgatga acatttggca agtctcaggt atgccggtat
      361 tatccagcag gccctgatgc ctgatgatga gcatcttgcc tcgctgttca gagatcattt
      421 tgtgtttttt cttccgcgtg acattgtaag cggagatttt tattatgcac tccgctacag
      481 gcattatatc tgtgttgcag cgggtgactg caccggacac ggtgttccgg gggctctttt
      541 aagcattatg ggcataagtt ttctgaatga gattctgcag gcaaggtgtg agccaagggc
      601 caacagggtt ctgaatttca tgagggaaaa agtgatgaag gctcttcatc agacgggtga
      661 gaaatctgaa gccaaggaca gtattgacat cgggctttgt gtatttgaca ccgagtcgca
      721 aaaacttcag tattcagggg ctaacaggcc gcttttaaga ataagggacg gggaagtctc
      781 ggagttcaag cccgacaaaa tgtctattgg tctggcaccc gttcatgagc atcctttcac
      841 gaatgtgatc atggataccc ggcccgggga tacattttac ctctatacag acggattttc
      901 ggatcagttc ggtgagctta ccgacaagaa gttcaagcat aatcagttga aaaaggttat
      961 tgcaggtgtg tcaggttttc ccatgagccg tcagagggag gttctggagg atacttttta
     1021 tgactggaag ggaaataccc cgcagattga tgacgtgctg gttttcggat tcagaatata
     1081 attaagcgga ttatgatgaa gctgatagct ttcaggatca ggaatttccg gtcgataacc
     1141 gataccggct ggcaatcact ttctcctgac aacataacct gtcttattgg tcagaacgaa
     1201 tcggggaaga cctctattct cgaggcgctg aaagtctttt ataccggagt catttccgag
     1261 gatgttctcc ggagcgatct ttccctcccg gaggtccact gccgcttcag gattccggaa
     1321 ggctggcttc tgaatataac tgataatccc ggtcccgagc tgagagagct ccttgccggg
     1381 cttgacagtc tggagctgac cagaacatgg gaagctgatc tgtcctctgt gattaaggtc
     1441 agtggtgcgg tcagccagta tcttgattct cttgaggatg cctggaagat ttaccttggc
     1501 gacgtgaccc tgaggcttga agaagagatg gacaatattg atgtgctgga agccactctg
     1561 gagaatatca gcaggcagga atccctgctg agttcccagc tgccgtcgag ggattccgcg
     1621 aaaaagcctt tttcattcct gtcgcggaaa aacgggaatg agagagcggc tgccggggtt
     1681 aatcccgaac tgtattccga gctggatgat cttcgtgaca aaaaggccag gatcacaagc
     1741 gagctggaaa gcaggaaggg gatgaagcag gccggggaaa aatggaagga gatcctcgac
     1801 aggtattcca tgtatgaaaa gcatatgagt gagctctttc tgaagcttga ggaaaggcat
     1861 cagaaactca cccttcttat gaagcctttt gatgatgagg atattgcatg gaagagtgtt
     1921 ctgcaggact atcgcaagac ccgtgaggac cgtgatatga taaagtcgga actggacagg
     1981 catattgcct tttccggata tcttctcaac ggttacagtg aggcggaagc agaggaaaag
     2041 gttggtgaga taatccagtc atacaaatcg cagtttaaca gtgagatgct gggcaggaaa
     2101 tattttgccc actgtcccgt atttgaattt ttcgaggatt tcagcagtct tcttccgaac
     2161 cgtattgatc tgcatgatat tatttcagga aatgaacagg ttgaaggata caaggctgca
     2221 aggaatttcc ttacgatagc caggctggat tattcatttt tccagcagcg gtcgagcagg
     2281 atactgaagc aaaagattga gaatataaat cagacgctta caacggattt ccaggatttc
     2341 tggcagcagt cgataggccg caacaataag atccacattc agtttgagct ggatcattat
     2401 gatgcgactg caggccggaa atcgggcaag ccctatcttg agttctggat caaggatgaa
     2461 ggcgaaaggc tttatcccaa gcagcggagc aggggagtgc ggtggttcct ttcattctat
     2521 ctggagctga aggcatctgc aaatattaac cgggaaatgg ttctcctggt tgatgaaccg
     2581 ggagttagtc ttcatgcacg ggcgcaggag gatgttctga aggttttcga ggatatcagg
     2641 gaaaggatac aggttgttta tacaacgcat tcaccccatc tggttgagat taacaagctg
     2701 caccgggtcc ttgccatcca gcgggatgat attgacagca tgcggagctc caccaggatt
     2761 ctggatcctc ttcagctttc ctcggccact cccgataccc tcagtcctct tcagagcatt
     2821 ctcgggaatc cccttcccgt ggaaggcgct gcgcccggca agttcaatct cattgtgaat
     2881 gacaacggaa cgttttatct catcaacgcc attctccatc ttacgggctt caagaacagg
     2941 ataagcgtga ttccttccac cggtgtttcg tcaattcccc tgctgtgcaa tatattgatg
     3001 gggtgggggc tcgatttcct ggtacttctc ttcaggaacg acatggagga agatattgtt
     3061 gaaaccttgc aggaaacggt atataaaccc gggagcaacg gaaaggaaac cattatcatg
     3121 atgccggaaa attttcataa ttctgaggat ctgttatcta cacttgattt taaaaatcat
     3181 attctgaaga ccagggaagg tatcacggtg tccaattccg attatatacg ggaaaaggaa
     3241 atgccacgca acttcgtttt ttcaagattc ctttcagagg ttatggatgg tgatatccgg
     3301 ctgtcggatt ttgacgagga gaccaatgag aatttcaggc ttttaatcga taagctgaaa
     3361 ggtcacaaat aaggctgaaa atattttgca cttaataccc tgtttcctgg gttttcgcag
     3421 cggggcagat aagggattgc tgccaggaga cgggagaccg ggccttattc cgttcgcaac
     3481 aacggaggac gacttgtttc aggcagtatt acaaagggaa ggagatttcc gccaatgttg
     3541 cccggtaaat aagggtccgg cagaccttaa caggttctgt atccatctat atctttaaaa
     3601 tctatctcat attccttgtt gtcagaaagg gtgatgttcg ctcccaacac cgaataagaa
     3661 ggggtaatat ccatgatctc tatctttttc acgggcgata attcttcttc tgtccattcg
     3721 ttatcatccg tcttatggag taataccgtg atcatcagtt caatggcggg attctttgcc
     3781 attctttttt tgtatgcata cagaacggta ctttcgtcgg cttcagcatt ccgtcccgtg
     3841 tgtatagttg ccgcaacttt atcccatccg ccataggtga tcaatgcaag tcttctgccg
     3901 ggaatggcag cagttataac tttttttgta ccctcttcga attgatgaat ttctgcttta
     3961 ttccccttaa tatgcggcag gccataatgc ccgagggtta attcatactc aaaagccaaa
     4021 cgggaacggt ctatcctaat aattcccccg gggataataa tttccgcgag gtctatgata
     4081 tatcccacgc cattattggg cggttttctc ataattgcct gcctgtacaa tacatttttc
     4141 cggacaccat tatacagcat actttgagat gtcgtaaagc ctgcattttt ttcaggatcg
     4201 ttttgcaggg tatgcccggt aagataaaag ttaatgtcat cgcctctcac atcacgcggg
     4261 tcaagactgc ggaagctata ttccattgat gttccgcctc cgggattatg atcttcccag
     4321 ggaaaatgag tgttatacac aagctttgaa taattatggt cgtcataata aacttttccc
     4381 ggaattattt cggaagttcc ggttttgcca tgatttacaa gaaccagtcc gggattttca
     4441 agaactgtcg ttttcgaatt gtttccgagc atttcccaga taccttcatt ttctttggct
     4501 gtccagaatg gcgaatcatc cggcaacgca aggcagatga acggcaaaaa catgatgaac
     4561 ggacttccgg cacaactata gttttgcagg acatattccc tgtgcccata aaaaccaaga
     4621 cttggaatat cgttttcata aaactcctca cgtgttacga actggagtaa agatcccgaa
     4681 caaagtcttc tggcccaacc cggatccagg ggcgatatac cttttagcat aaaggaaaca
     4741 gggaatcctc ccgaaatcca tgtcctgtaa catatactcc tagcccacat attcacatag
     4801 ccatctctgg caaaaaaatc agtgtatgtt ttcattaatt cgtaagcaga tttttcaatg
     4861 gtttctgcta tttcgggata atactcatcg ccaaacgccc tgttccatat tgttccgtaa
     4921 acgataaaca ggcttattgt ataataattg taagtttgtt caaggtacca cccgttaccc
     4981 gagtggtagg aggccaccca taaaagatgg cttttcagca gctcctcgtc aatttcgtaa
     5041 ccatattttt tgaggaaaga aagcgtaaca atgttaaaaa ttctccagtt gttctgggta
     5101 gttctgtgat gtgcccattt tgacatggtt accgctatct cgtctctttg ttcccgggta
     5161 aaatgaggcc aaagggtttc aggcatgagc agcagggttt tgaacaaacc tccaaactca
     5221 catgtgaact gataagtgga atcgggcagg ttttcaggta aaggcaatga attcggatgc
     5281 cccggggtga gcgcgttata aatatgccgg caatagtaat cccttaaatt gatattatta
     5341 atttttgtat cgggatcaac atggataagc ggtcccgcta gtgtgaaagt cctttcgagg
     5401 gcttcaaaat cggccgaacg gtaacgccag tcgggagcat ccggttgtgg atatgtttta
     5461 ccgggaattt tcggaaagga aatgggggtt tcaatagacc ggacatgctt aaatgccctt
     5521 tccaataaat attttgcgca ttctatgtag tgtttttttg tcattcctgt aaagggactt
     5581 aatttccagt caggattctt tacttcgaaa gtatttttca tcttatgttc cattttaaca
     5641 atgagtccgg tctgaaaagg taatttttaa atacagggat ttaacttcac gtttaactgt
     5701 taatttaagt gagcgattta tgataaaaaa caaaagacag gttgtgtatt ggttcaggta
     5761 tgtatatgtt agagattata atactgttta gcaacacatc accggttagg tgcacaacac
     5821 tatcctgtat gagtaccaag ataaaaaaaa atggcataac aaacggaaaa atatctgcct
     5881 gtgggggctc ggtttcctgg tatttctctt caggaacgac atggaggaaa tattgctgaa
     5941 accttgcagg gaacggtata taaacccggg agcaacggtg aggataccaa tgagaatttc
     6001 aggcttttaa tcgataagct gaaaggtcac aaataaggct gaaaatattt tgcacttaat
     6061 accctatttc ctatttttgt cgcagcgcag cagacaggag attgctgtcc tttacatgtt
     6121 ggagagatgg gtgagtggct taaaccaaca gtttgctaaa ctgtcgtatg ggtaactgta
     6181 ccgggggttc gaatccccct ctctccgcag aatgcctgaa taccagcccg ccttccggcg
     6241 ggcgttttgt tttgttaaat cagggaaggg atccgtagtc tgcaggcgtt tcaaccaaca
     6301 gcagccatct gaccttaatc caacccaacc ggaaccgcgt tatgaaatga tttgggaatc
     6361 attatttgca aggactggca attattttca gtagcgcgtt gttaaaaaac ctgtatttta
     6421 cctattttta aaaaaagtaa tggctgtgta tggccggccg gctgaaataa agcgaatatg
     6481 caatccggtg acaggaaaac agatatgttt cttgttgcac aacagcctga aacagaaatg
     6541 gatttacgga ttaaaacctg ataagaagac aaataatcat tcatatattt gaaaaaccgg
     6601 gataaacaat gaaaccagta ctctaccttg ttcttttttc tctttttata atattctcat
     6661 gccgtgaagc agaaaggatt ccggcgataa ttttcgacac tgacctggga cctgattatg
     6721 atgatgccgg tgccatggcc gttcttcatg caatggctga cagcgggaaa gcggaaatcc
     6781 ttgccaccct atcttcgaac aggcatgaac ttgtagcccc gtcgattgat gtaataaata
     6841 cctacttcaa ccgcccggat cttccggtcg gagcaccgaa gaccggggga atatccatgg
     6901 gttcgggaca gcactgggcc gactccattg tatcagtgta tcctcaccgg atcagatcga
     6961 ctgaagaggt tcccgatgcc gtggaggttt accgcaggat actgagtgac cgtccggaca
     7021 ggagtgttac cattgtaacc gtaggctttc ttacaaatct gagcaatctt ttgatgtcgc
     7081 agcctgatgt ttacagtccg ctgaacggac gtgaactggt tgagaggaag gtaaagaggc
     7141 ttgtatcgat ggccggatct tttccggaag gcagggaatt caatgtctat accgattccg
     7201 tggcttcaaa gcatgctctg gacaactggc cgggagaaat catattcacc ggttttgaaa
     7261 ttggatcaag gattttaacc ggattgaaac ttattgccgc ggggattaaa gacaatccgg
     7321 tcagggatgt gtaccgcatc agcattcccc tgtctgccgg agacaaatat ggcaggatga
     7381 gctgggatca taccgctgtt ctgattgccg tgtacggtac cggaggattc tttgacacgg
     7441 tgaggggaag gattattgtg aacagtgatg gttcaaatgt ctgggagaat gatgctgaag
     7501 gccctcattc atatgttgtt cagaaaatgc cggctgatga ggtggcagct tttatcgagt
     7561 cgaggatgat gcatgttcct gaaaggaaat aaatatgctg tgagttgcag gatatgtttt
     7621 ttcgttcagc agtttcatgg ggtcttcggg attgatttca tagctgaatc ctctttctga
     7681 agccttcatt acaaggactt tgaaaaggag aaggtttgca agaatggatt cagccctgca
     7741 ggatgatatt tttgcaatct ttttgaattt tgaaatgctg attgatccgt tttcggccag
     7801 gtaatccatc agtatatttt cggccttgct gaattttacc attaccccag ttttctttcc
     7861 ctccttgcgc catacctgga gcaggacccg gttggccaga aggttttggt cgttgtgcct
     7921 gaagtatgtc agccatttcc cgttttcgtc ctgtgcctgg taaggtcttt tggttccttt
     7981 ggacacttca acttcaagta ttgttttcac accaacagca tgttgtttta tggtgtagtt
     8041 tatttccggg cggcagaata tctgggctgc agtgtcgacc ataaaaattt cctcttccga
     8101 ttgtattcct gctatgcttc cgttatccct tacgccaatc agcaatctcc cgccgtcagt
     8161 atttgagaat gcagataatg ttcttgctat tttcctggag tcggatacac aatacttgaa
     8221 atcgacatac tgattctcac cctcttcaat cagttttctc agatagtatt ccatctttcc
     8281 ggttttattt taaggattcc ggacaagcgc cttcagcggc ggttcctttt ccctgttaca
     8341 gcaatattcc tgccagtgaa gcggagatat atgatgccat tgttccgcag agcattgcct
     8401 tgaatcccag tctggccaga tcggctttcc gggttggtgc cattgcaccg attcctccga
     8461 tctgtatggc tattgaactg aagtttgcaa aaccgcaaag tgcaaagctt gcaataacta
     8521 ttgccttggg acttaagctg tttgccgcga tcataggagc cagaccggta tatgcgacga
     8581 attcgttaat aaccattttc gtgcccatga gggatcccac ctcaaatgat tccgctacag
     8641 gcacacccat tataatagca aaaagggaaa agacgagtcc gaatacatcc ttaagtctta
     8701 aattttcaat atccagtccg ataaaatcaa gtgaggcgcc cagaagtgaa aggagctgtc
     8761 cgagatagcc caggatggca tcagcgagtg ccacaagagc tatcacgccg atcagcattg
     8821 caatgacatt tgtggaaatt ttccagccgt cagatgctcc gtgtgagatt gcatccatta
     8881 tgtttgcatg ttcctttttc acttctactg ttaccttacc ctttgtttcg gataccttcg
     8941 tctcgggata aacgattttt gatatcacca gtgctcccgg agcggccata aggcttgctg
     9001 ccagaaggta tgatgcagga acccccatgg atatatatac agccattact ccgccggcta
     9061 tgcatgccat gctgccggtc attgacgcca gcagttccga catggtcatt ccggacagat
     9121 aaggccttat cattatctgg gcttccacct ggccgacaaa tgcacttgcg acatttgaaa
     9181 gagcctcact tccgctgacc tgcatcaccc agtgtacaaa cctggcgata agggcgacaa
     9241 ctctctgcat caatcctata tgataggcca ttgcaacaag gacgcagaca aatattattg
     9301 tggggataag gataaaaagg aaaacacctc ccgagacaat tgtcggagga agaatttcct
     9361 gaacctttgt cagatcgccg aatacgaatg atccgccttc ctgggagaag tcaagcagtt
     9421 tatttatggc tttcccgagc cagtgaaata tttcctgacc tatggggact ttgagaatga
     9481 atatggcaag tgcaatctga agtcccagac cggtaaacac aagtctgtaa ttaatcgcct
     9541 ttctgttatt cgaccataaa aatgcaaggc cgagaatcac tattatcccg atgatcccgg
     9601 taaatcgtgc catgtaagta tgaattttca aagagtatga gccaaaccta catttttttt
     9661 cgatacacag gtatcacaac cggaaacaat ttttacctgt atattcctgc ctgtccaata
     9721 caggaaagtg cccagccaaa agaaacaatt cttacctgta tattcctatc tgtccacgat
     9781 agggaagcgc cctgtaaatt cttttcacat ctcctcttgc cagtctgatt cttatggcgg
     9841 gatagtattc gggaaccctg aatgtgattg tccgggacaa tgaagctgca gttgtttcga
     9901 gtctgtctct gaggtcgaaa agaaagcggt ggaaattatc cccttttttt aatttctcat
     9961 cctggtacac gtgaagcttt aaaccgtttt ccatcagaag aatgaaattc accggctcaa
    10021 aatctgtggt gtccggaatc atgtcgggct gatattcaga tgtatccctt ggagccattt
    10081 ttatcatcag gggttccttt ctgattgtgg agacatccga agcaatggtg aaagggctgg
    10141 aaagcatgtt caggattgca tactcatctc tttggaggat actgcttatc cttttcctga
    10201 gaattctggc cgtatggaca acaacaccgc ctatttccag atttacagta ctgtcggcaa
    10261 ggtttaccgt catgtagatt gaatcgctgg cagccattgt gactcttgac tggtagtatg
    10321 ccttttcctt cattagggca aggtatgttg gatcagcaag aattctttcg tcaggagggt
    10381 tttttatgtt tttatccgga ccgtatgctg ttctcagctc attaagtttt ttctccggcg
    10441 ctgacattga aagaacaagg tgtatgatca tgaaaatgac tgcaactgtc agtatgaaga
    10501 tatgaagctt tttcccggcc ggaggggatt gtaattcttg tttcatacac tgagattcag
    10561 aagatatata cttttgatcc tatattcagt gttctgaaga ttacttccag atcttcatca
    10621 cccagtctga cacatccatg agttaccggc atacctatca gccttttata aattgttccg
    10681 tgaatcaggt atccgtcgcc gaggctcagg gaatattttc caagtacccc gtgttcatat
    10741 ctggaaggat ggttgtgagc cggtattggc agtccttcct ccacgaaagc ccagtcgggc
    10801 ctggtccata caggattaac ggtttttgca cggatccgga accggccttt gggcgttttg
    10861 aatatccagc ttctgccttc gtgtgtctga agcatcttat agcttcccga gctgcagggg
    10921 cctgttctta tctctttttt attccggtac aaatggaaaa tattgtcagt agtgttgatt
    10981 acaaggtatg actgtcctgt ggtataggaa ttatatcttc tttcaagtct ttcggtatct
    11041 gttctgatgc gggcaatagt cttctgatac gaaggatctt cttttacttc tgatgcggca
    11101 ggaggggatg ccacccttcc gcagcagagt tcctgtgcca gggggactat ccacagaagg
    11161 aatatgatca ggaaggcaat ggctgctgaa aatattgcag acatgtatat tttcaggaga
    11221 acggcgccaa gtttttcatt ctccctgatt ttttcaaaaa attgaatgac cagtgatttc
    11281 agtttgctta atatgttcag agcagtcttc cggattctgt ctgcttcaag gaaccggatt
    11341 ttcttcggat ctgtcatgct ttgagattca attttcaaaa atctttttct gatcatatag
    11401 tgatcccacg attgttaccc gtgttccgac ccttacatat ggaaaaagcg cgtcaatatc
    11461 attattggca agtgcaatgc atccttcggt ccagtcggct ccccttccac ctccgccatg
    11521 gatctcgatc attccgccta tatcagctgt gggaggcagg gcgccatttc tgatatcccg
    11581 tttaaacctt gccatatcct cactgttcgg atagttaagc aggagtgccc tgtggtattt
    11641 tgttccggat ccttccagtt ttttaattat ttggtacata ccttcaggag ttgccatatc
    11701 tcccataatt cttttactgc ctatccagtt tctacccagt tctgccgaat attcccttat
    11761 tttttttcct ccatggtaca gaatcagctt ccttgcgaac ttatcgacaa ttattgcata
    11821 acctccggtc ttttccgatt cagataatgt tagggctatc catttttccc attgagggaa
    11881 ggattgaaaa tactctctta aattgagatc tgcaaactcc cgtgaggaca caagaaggtt
    11941 ttcagaatcc gtaatatagg aatctgcatc aatatagttt ccttcagtaa aagctgtttc
    12001 ggcatctatc aggagcattt tcccccgtgt tatccgttcc ctgatttcga aagaataggg
    12061 gaagctgctg taatgatcat taatctcttt cacaatttta ttcagggtat caatgatccg
    12121 ggcctggccc tccttcattt cagctttgag cagggcagtc cttatatttg ccgaatctga
    12181 cattcctgcc gataatagtg catattctct taccaggtcg aatttgcgga agaggatgaa
    12241 ttttctgttt tctcttctcc agattgccat tccggagtcg caggacacag atgcctgatt
    12301 gtaaaactcc ggggtatagc ggtctgcctg atttcttttt gccacagaca aggattccac
    12361 tgcgaatatc atttcgcctg ccgggggttt cgggagaaaa aaaatgaaaa ggaatccggc
    12421 agacagcagt gctgtcattg ttaacaaaaa gatcagcaga ttccgttttc tttttctcat
    12481 accgggatat tcagtcggaa agaagttcgc ggacacaata cagttcaggt atcagaatcc
    12541 ggataagagc cgcctgttcc cagtggacag tgacaaactc tgaaacagga attatgtatc
    12601 agtaatttct gggagtaccg ggacggctca tccggatctt ccgccatacg tttaataatt
    12661 tttccgcgtc agagtgattt tctcagcaga gtcagaatct gattcttgct tttgtcagga
    12721 cttccttcat ttcactgtta agtccgtcag cttttgccct ggctgctttt actctgttaa
    12781 gcgcatccat gaatgcacct ttgtcgaaga gattctgggc ttctgagact gagttttcaa
    12841 caccggaaag gtcggctttg atctgttcaa tgacctgtgc accttcttta cctctcggaa
    12901 gcctggttac gagccttgag ttttccgcaa ccacgttcct ggtatcattc aggaggttct
    12961 ggacttcctg ttttacttcc tcctttttag catctactct ggcagctgtc tggttagcca
    13021 tatttgttac ctgatcaagt ctgtcctttt cgtcaccgaa atttttgaag agtttggatt
    13081 tctggagttc aatatttacc agaatggcat tcattgaatc ctggattgct gcaaactcat
    13141 cagggagata tatgtcagcc tggaccgtcc tggctgcttc aatagctgca tttgttgcat
    13201 ctatctgggc ctgcggaact tttccgcaac ctgcaagaat tccaatcatt actatgactg
    13261 caaatcccat caacattttg tttttcatct tttcaattgt ttttaattct gaacttatct
    13321 gaaactttct gatttatccg ggtcattttc cattgatccg gaaaaaatcg ttgcaaaatt
    13381 atatcatttt ctttgaattg acagtaaggg tataaaggaa aatttttata gaaattttat
    13441 ttgaaaaaca ctggtaaata aagtttttta attcatttaa atctggttca ttatccggtt
    13501 taatccttat aggggtcata tatttccggt cctgccattg ctactccgac cgggtgaagc
    13561 ctgaagagaa tttttattgt atctccctga gatttcagca cttcatccag ttttttgtag
    13621 caat
//
LOCUS       DAOSPX010000052        13589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4614_length_13589_cov_4.018472, whole genome shotgun sequence.
ACCESSION   DAOSPX010000052 DAOSPX010000000
VERSION     DAOSPX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13589
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4614_length_13589_cov_4.018472"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLZ75_04200"
     CDS             <1..322
                     /locus_tag="PLZ75_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOS71632.1"
                     /translation="KLDGVSVRVPTPTGSLVDLVAVLKKEVTKEEINAAMKKAAEGPM
                     KGILEYTEEPIVSVDVIHTEASAIFDALSTMVNGKMIKVMAWYDNEWAYSCRVVELIP
                     LLMK"
     gene            complement(414..1655)
                     /locus_tag="PLZ75_04205"
     CDS             complement(414..1655)
                     /locus_tag="PLZ75_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS71633.1"
                     /translation="MKRGVVKENLRVSLRAIRSNQLRTILTICIIAFGIMALIGILTA
                     IDAIKNSLTDQFTMMGANSFSISSRGMNIQVGNDRIRTRNFSRISYRQAMEFKSLFAE
                     PAWVSVSFRATDMATVKYGREETNPNIGIMGVDENYLTVSGYEIEKGREFTAEELRLN
                     RRIILLGSVIAADLFPTGIDPLGQEVSIAGLKMKVVGVLKSKGASLMSDDNVCFIPIS
                     TARYYFSRPNLNYRINVMPMNPVDLDVMAGEAEAVFRIVRNLDPRDETDFNISKSDSI
                     VALLLKNIKFITLAATLIGIITLFGAAVGLMNIMLVSVTERTREIGVRKAVGAKPSTI
                     KYQFLYESVIIGQLGGLFGITLGIIIGNLVASALKSHFIIPWLWILLGVAVCFIVGVV
                     SGYAPAVKAAGIDPIEALRYE"
     gene            1881..2090
                     /locus_tag="PLZ75_04210"
     CDS             1881..2090
                     /locus_tag="PLZ75_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71634.1"
                     /translation="MKKQVTIFPPEIISRDITGRRRRRRRREREKERKREREKERKRK
                     GEKEKRRIRRKRKREGEKENSENYL"
     gene            2167..2547
                     /locus_tag="PLZ75_04215"
     CDS             2167..2547
                     /locus_tag="PLZ75_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOS71635.1"
                     /translation="MRELTRAEEQVMQVLWKLEKGFVKDILEHFDDPKPAYNTVSTIV
                     RILQNKGFVSYRAYGRTHEYFPLITKDEYSKFHMSSFIQDYFSNSFGKMVSFFAREKH
                     ISLREMEEIMQIMEKEVKKQKPEK"
     gene            2544..4253
                     /locus_tag="PLZ75_04220"
     CDS             2544..4253
                     /locus_tag="PLZ75_04220"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOS71636.1"
                     /translation="MNPLLIYTIKASFCLASFYLFYILFLDRDTLYSRNRIFLLLSLA
                     VSHLLPFIVIPLKQAGEIQFAGIDLSGIMLNTRAEPSETGSTIHSSINTGSVLNAIYL
                     TGVIILSLTLVFEMLKLLFLIIRKKEKDDRIIRLAGNRITGFTAFGYIFIDESADSGE
                     EEIIRHEQKHLESGHFYDILLMELIRIFQWFNPFVYLYNRSLRAVHEFEADEKCLSAG
                     ASPASYQGLILDQVFRTKVFSSANRFSHPTLIKKRMIMMTKKRSGKLASLKILMVVPV
                     VIFLILIISTCSKQSFDSFSGSVTSTAPQQAEQAAALSAVMAPVIEGDAYVVVEQMPM
                     FPGGDIALLRYIAENTSYPEEAKAQNIEGRVIIRFMVLEDGTVSALSVIRGVDPVLDS
                     EALRVVSTVPKFEPGKQNGVAVPVWYMVPITFALPDKSGSDRQAPPALQPSQAGAVME
                     VSVPGSEEPFVVVEEMPLFPGGDAAILQYLADNLRYPAAARENKITGRVIVRFCVNAT
                     GQVDRVSVLKGVNPELDAEAVRLVSTLPAFRPGRQNGRAVPVWYMAPVNFGTEETKTG
                     SPE"
     gene            4394..5437
                     /locus_tag="PLZ75_04225"
     CDS             4394..5437
                     /locus_tag="PLZ75_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOS71637.1"
                     /translation="MKLAERIETFASLGESLRDALAGKQTGHSRRLNELIEKQYLKNP
                     WFTPPGVRLAVSAIASELVHDRLSHWCSAYPALDEEITPANVGVVMAGNIPLAGFHDF
                     LSILISGNRIIAKKSSKDPDLIELIADIVISLNPAFGTMISLTEDRLSGFDMIIATGS
                     DNSARYFEYYFGRYPHIIRRNRNSIAIIDGTESKGELRELGNDVFSYFGLGCRNVSLV
                     FLPEGYDIRDIISVWHEFSSAGNHNKYAGNYDFHKAVFLVNRQEFTDTGFLLLKEDNS
                     LSSPVSVLHYSFYKTAEELQSWISTRKDKIQCITGHGHVPFGRSQMPALWDYADGVDT
                     IEFLLKKKQRGIL"
     gene            5479..6039
                     /locus_tag="PLZ75_04230"
     CDS             5479..6039
                     /locus_tag="PLZ75_04230"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71638.1"
                     /translation="MVYKFVVLSDEDESFMREFEFLDSQTLLDFHNLIQEELEFDKSQ
                     MASFFMAADNWEKEEEFTLFDMGTGSSTMEDTVLEDIIFHKNQKLLYVFDFFNDRSLF
                     IEYTGEVEEIDGREYPVCSNSKGIPPKQVVFGSSSKKLYRNIIVSDDDDDDDEPDMDE
                     LFLDDDDDVLPDFDNDEPMIDDEDDD"
     gene            6058..6972
                     /gene="miaA"
                     /locus_tag="PLZ75_04235"
     CDS             6058..6972
                     /gene="miaA"
                     /locus_tag="PLZ75_04235"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOS71639.1"
                     /translation="MKNILIVLLGPTGVGKTDLSIDLAVHFRCDIISADSRQFYREMN
                     IGTAVPSPDQLSRVKHHFIGFLSVKDYYSASLYERDVLRLLPSLFARNPLVLMTGGSG
                     LYIDAVCRGIDDIPDTDQAIRRKYVQKYREEGIESLRMLLRMLDPVYYEKVDLKNPRR
                     IIRALEICESTGRPYSAFLTKKEQPRNFEVIRIGINRPREELFGRINRRVDEMIAAGL
                     EDEAERLYSLRGINALNSVGYTELFEYFSSRITREQAIGLIKRNSRRYAKRQLTWWNR
                     DREIAWFGADEKNRITEYLSNKINSLSE"
     gene            complement(7090..7746)
                     /gene="rpe"
                     /locus_tag="PLZ75_04240"
     CDS             complement(7090..7746)
                     /gene="rpe"
                     /locus_tag="PLZ75_04240"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOS71640.1"
                     /translation="MNHLVSPSLLAADFGNLSEDVRLINGSMADWIHCDIMDGVFVPN
                     ISFGFPVVEHVRKVAEKPLDVHLMIVDPDRYLVRWKETGAAILTVQYEACVHLHRTVT
                     EIRRLGMKAGVAVNPHTPVALLKNILHTVDMILIMTVNPGFGGQVFIEESYGKIEELK
                     YMIRTGGHDVLIQVDGGIDTHNAAELVETGADVLVAGNTVFSSGDPADTIRKLKMLEE
                     "
     gene            complement(7936..8799)
                     /locus_tag="PLZ75_04245"
     CDS             complement(7936..8799)
                     /locus_tag="PLZ75_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOS71641.1"
                     /translation="MRQLKITKQVTNRDTPSLDKYLQEIGRVELISPEEEVILARKIR
                     AGDTEALKKLINSNLRFVVSVAKQYQNQGMSLPDLINEGNLGLMKAASRFDETRGFKF
                     ISYAVWWIRQAILQALAEQSRIVRLPVNKIGSISRINKAMIRLEQEFEREPTSLEIAE
                     LLEMIPEDVRESLRTNGRTVSMDAPISAEEDNNMYDVIQNDEIPSPDRNLINESLSYE
                     IERALSTLSPREAKVLKYYFGLGMKHPLTLEEIGEKLHLTRERVRQIKEKAIKRIQFT
                     TRCKILRTYLG"
     gene            9151..10884
                     /gene="rho"
                     /locus_tag="PLZ75_04250"
     CDS             9151..10884
                     /gene="rho"
                     /locus_tag="PLZ75_04250"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOS71642.1"
                     /translation="MYDILELSKKLLPELREIAKTLNIKKADSLKKQDLVYKILDQQA
                     ITSTESITIQKKEDPPQGKSEPEPVREKNQRRGKRPRTVKPLFKSPTESQAASSQGAG
                     RTTAALAGNEMADERRSPAIRPEPKSEEKAAGSGKRRNSGADTAVTEAVPETAEGNTS
                     AGDDQAAEKTMTTGTPASEPVTAEAAAAPRPAPAREEKKEKPYEFEGIIYNTGVLEIM
                     PDGYGFLRSPDYNYLNSPDDIYVSQSQIKLFGLKTGDTIKGTIRPPKEGEKYFPLIKV
                     DEINGRSPDYIRDRVPFDFLTPLFPNEKFTLCTPGEGSLSVRVIDMFTPIGKGQRGLI
                     VAQPKTGKTILLQEIANAIAKYHPEVYLMILLIDERPEEVTDMARNVKAEVIASTFDE
                     PAERHCRVANIVLEKAKRLVECGHDVVILLDSITRLARAFNTTAPASGKVLSGGVDSN
                     ALHKPKRFFGAARNIEDGGSLTILATALTETGSKMDDVIFEEFKGTGNMELQLDRKLS
                     NRRIFPSIDIPASSTRREDLLLNKNIMQKIWVLRNYLADMNSLEAMEFLRDRLMQSRS
                     NEEFLISMNGG"
     gene            10959..>13589
                     /gene="nifJ"
                     /locus_tag="PLZ75_04255"
     CDS             10959..>13589
                     /gene="nifJ"
                     /locus_tag="PLZ75_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOS71643.1"
                     /translation="MGTGKFIRCDGNQAASHIAYMFSEVACIYPITPSSTMAEFVDEW
                     SANGLKNMFGEQVKVVEMQSEGGAAGAVHGSLQAGALCSTFTASQGLLLMIPNMYKIA
                     AELLPGVFHVSARTIAAHALSIFGDHSDIYATRQTGFAILATGSVQEIMDLGGVAHLS
                     AIKSRIPFLHFFDGFRTSSEVQKVRLMEMEDLKKLVDFEALERFRNNALNPEHPVTRG
                     TAQNPDIFFQAKEAINRFYEPVADIVNDYMTEISKITGKDYRPFTYYGAPDAENIIIA
                     MGSVTDTAKEVIDYLAEKGEKAGLISVHLYRPFSAKYFFNVLPSTVKKITVLDRTKEP
                     GAPGEPLYLDIRDLFYNRSERPLIIGGRYGLSSKDTTPAQVLAVYENMKLAEPKNQFT
                     VGIVDDVTHTSLPLLPEINVAPKGTYSAKFYGLGSDGTVGANKNSIKIIGDTTDKYCQ
                     AYFAYDSKKSGGITISHLRFGDNPIRSPYYVNTPDFVACHVPSYLDKYDMLKGLKKGG
                     TFLLNSIWDAEETKKRLPDHMKKYLAVNEINFHIINATLIAEEIGLGSRTNTIMQSAF
                     FRLANVIPYDKAEEEMKKAIYSSYGRRGEEVVKMNYEAVARGSQVEKVEIPAEWADIV
                     IKKAEDKRDIPEFIRDFAEPVNMLKGDDLPVSAFVGREDGTFPAGTTAFEKRGIAVNV
                     PEWQAEECIQCNQCSYVCPHAAIRPFLLTEEEAANAPEGTKTLQGIPKTLNEYRFRIQ
                     LSLYDCTGCGNCADVCPSKNKALIMKPFETQLEEGPRWTYMHEKVGYKDHVVDKFLNV
                     KNSQFAQPLFEFSGACAGCGETPYIKTITQLFGDRMIIANATGCSSIYGGSAPNTPYT
                     VNKHGHGPAWANSLFEDNAEF"
BASE COUNT         3960 a         3146 c         3118 g         3365 t
ORIGIN      
        1 aaaactggat ggcgtttcag tgagagtccc cactcccacg ggttcacttg ttgaccttgt
       61 tgccgtactg aaaaaggaag tgacgaagga ggagataaat gcagcaatga aaaaagccgc
      121 tgaaggaccc atgaaaggta ttctcgaata taccgaagaa cccattgttt ccgttgatgt
      181 gatccatacg gaggcatcag ccatctttga tgccctcagc acaatggtga acggaaaaat
      241 gatcaaggtg atggcatggt atgataatga atgggcatat tcatgcaggg ttgtcgaact
      301 gattccctta ctgatgaaat aaacgggaaa ttttcatgtg agaccgaacg gatggtcttg
      361 catgcaatgc cataacaatg aagccgggat actggaaaca gtgtcccggt ttttcattca
      421 tagcgcaaag cttcaatggg atcgataccg gcagccttta ccgccggggc ataaccggat
      481 accactccaa cgataaaaca aactgcaact cccagcagta tccacagcca gggtattata
      541 aagtgagatt tcagggcaga tgcaacaagg ttaccgatta tgatgcccag tgttatgcca
      601 aacaggcctc ccagctgtcc gataataacc gattcataaa gaaactggta ttttatggtg
      661 gagggcttgg ctcccactgc ctttctcacg ccaatctccc gggtgcgttc cgttaccgat
      721 acaagcatga tgttcatcag gcccacggcg gctccgaaaa gggttataat gcctatcagc
      781 gttgccgcca gggtgatgaa ctttatgttt ttcagcaaaa gggccacaat ggagtcactt
      841 tttgaaatat tgaaatccgt ttcatccctg ggatccaggt tgcgcacaat cctgaaaaca
      901 gcttccgcct caccggccat cacgtcaaga tcaacaggat tcatcggcat tacgttgatg
      961 cggtaattca ggttgggacg tgaaaaataa tacctcgcgg tgcttatcgg aatgaaacag
     1021 acattgtcat cgctcataag gcttgcaccc ttgctcttca gcaccccgac aaccttcatc
     1081 ttcaggcctg caatggaaac ctcctgtccc agcgggtcaa tacctgtcgg gaacaggtcc
     1141 gcggcaatta ccgaaccaag caggatgatc cgcctgttga gccttaattc ctctgctgta
     1201 aactccctgc ccttttctat ttcataaccc gataccgtta aataattctc gtcaaccccc
     1261 ataatcccta tattgggatt agtctcttcc cggccatact tcacggttgc catgtcggtt
     1321 gcccggaagg ataccgatac ccatgcaggt tcggcaaaca gggatttgaa ttccattgcc
     1381 tgcctgtagg aaatacggga aaagttcctg gtgcggatac ggtcatttcc cacctggatg
     1441 ttcatgcctc tcgatgatat gctgaatgag ttggcaccca tcatcgtaaa ctgatcagta
     1501 agcgaattct tgatggcatc aattgccgtg agtataccta tcagagccat tatcccgaaa
     1561 gcaattatac atatggtcag aatcgttctc aactgattcg acctgatagc cctgagggaa
     1621 accctcagat tctccttcac cacacctctc ttcataacgg gataaaatga aacctgactc
     1681 aattttaaaa aacagaataa aagtacacat ttttaagcat agcttatacg gggctttcct
     1741 caaattaata ccggacatct ctcaaattaa taccggccct tctccttcct gtccaccatt
     1801 acattaattc cggatgcgga taaatatttt catcaaatac ttaacgattt agttgtataa
     1861 ctaaatattt cgttgtaaat ttgaaaaaac aggtaacaat ttttccgccg gagataattt
     1921 cccgggatat aaccggaagg agaaggagaa ggagaaggag agaaagagag aaagagagaa
     1981 agagagaaag agagaaagag agaaagagaa aaggagaaaa ggagaaaagg agaataagga
     2041 gaaagagaaa gagagaagga gaaaaagaaa atagtgagaa ttacctttaa gggaaaacca
     2101 gtggcagcat aatacaatta aatcttaata ataaaaaccg gtcgccagac tattaaacaa
     2161 cttaaaatga gagaacttac cagggcagaa gagcaggtaa tgcaggttct gtggaagctt
     2221 gaaaagggat ttgtaaaaga catcctggaa catttcgatg atccgaaacc tgcatacaat
     2281 acagtcagca caattgtcag gatattgcag aacaaggggt ttgtctccta ccgggcctat
     2341 ggcaggacgc atgaatattt cccgctcatt acaaaggatg aatattcaaa attccacatg
     2401 agttcattca ttcaggatta tttctccaat tccttcggca agatggtcag tttctttgcc
     2461 agggaaaaac atatttccct cagggaaatg gaggagataa tgcagatcat ggaaaaggaa
     2521 gtaaaaaaac aaaagcctga aaaatgaatc cactgcttat atacacgatc aaggcttcat
     2581 tctgccttgc ttctttttat cttttttaca tcctgtttct ggacagggat accctgtaca
     2641 gccgcaacag gatcttcctg cttttatcac tggcggtgtc ccaccttctt cccttcattg
     2701 ttattccgct aaaacaggcc ggcgaaatac agtttgcggg aattgacctc agcgggatca
     2761 tgctgaacac cagggccgaa ccttcagaaa ccggcagtac aatccattcc tccattaata
     2821 ccggcagcgt cctgaatgcc atttacctga ccggggttat cattctttcc ctgacacttg
     2881 tcttcgaaat gctgaagctt ctttttctga tcatcaggaa gaaggaaaag gatgacagga
     2941 ttatcaggct ggcaggtaac agaataacag gattcacggc attcggatac atatttattg
     3001 atgaatcagc ggacagcggg gaggaagaga ttatcaggca cgaacagaaa catcttgaaa
     3061 gcggccattt ctatgatatc ctgttaatgg agctcatcag gatctttcaa tggtttaatc
     3121 cctttgtata cctgtataac aggtccctga gagcggttca tgaatttgag gctgatgaga
     3181 aatgtctgag tgccggagct tcgccggcca gctaccaggg attgattctc gatcaggttt
     3241 tccggacaaa agtattcagc tctgcaaaca gattttcaca ccccacactt ataaaaaaac
     3301 gaatgatcat gatgacaaaa aaacgttccg gaaaactggc cagcctgaaa atactcatgg
     3361 tagtgccggt agtaatcttt ttaatactga taatttccac ctgttcaaaa caatcctttg
     3421 attcattttc gggcagtgta acatccaccg cgccgcagca ggctgagcag gccgcagcct
     3481 taagtgcagt catggcacct gtcattgaag gtgatgcata tgttgttgtt gagcaaatgc
     3541 ccatgttccc gggaggggat atagccctgc tgagatatat tgctgaaaat accagctacc
     3601 cggaggaagc aaaggcgcaa aatatcgaag gaagggtcat tatccgcttc atggtcctgg
     3661 aagacggtac ggtttccgct ctttcggtga taagaggagt tgacccggtt ctggacagtg
     3721 aagcactgag ggttgtaagc accgttccga agtttgaacc cggaaagcag aacggtgttg
     3781 cagttccggt atggtatatg gttccgatta catttgccct tccggacaag tccggttcgg
     3841 accggcaggc tccgccggcc ctgcagccat cacaggccgg ggcagtaatg gaggtcagtg
     3901 tcccgggatc agaagaaccc tttgtggtgg tcgaggaaat gcctctcttt cccggcggcg
     3961 atgcagccat ccttcagtac cttgctgata atctcagata ccccgcagcg gccagggaaa
     4021 ataaaattac cggcagggtt attgtccggt tctgtgtaaa tgcaacaggt caggtcgaca
     4081 gggtaagcgt cctgaaagga gttaacccgg aactggatgc tgaagcggtc cggcttgtat
     4141 caacacttcc ggctttcaga cccggcaggc agaatggcag agctgtcccg gtgtggtata
     4201 tggctcccgt gaactttggt actgaagaaa caaaaaccgg ttccccggaa taattccccg
     4261 gagaattttc cccggaaaga caatggcggg aaaacattct gacgcgcttt ttcattccac
     4321 tatgccatta aaactgatct cctgacggga tcagtttttt atttcagtaa atttgccggt
     4381 caattaccgg atcatgaaac tagccgaaag aatagaaact tttgcatctc tcggagaatc
     4441 cctgcgcgat gcgcttgcag ggaagcaaac tggccacagc agacggctca atgagctcat
     4501 agaaaagcag tatctcaaga acccctggtt cacacctccc ggtgtaaggc tggctgtaag
     4561 cgccatagcc agtgaacttg tccatgacag actcagccac tggtgttcgg cttatcctgc
     4621 tctggatgaa gaaatcactc cggctaatgt tggtgttgtg atggcaggca atattccgct
     4681 ggcgggattt catgatttcc tgagcatcct gatatccgga aaccggatca ttgcaaaaaa
     4741 aagctcaaag gatcccgatc tgatcgaact gattgcagac attgtcattt cccttaatcc
     4801 tgcattcggt acaatgattt ctcttactga agaccgcctc agcggctttg acatgataat
     4861 cgcaaccggt tccgacaaca gcgcccgtta ctttgaatat tatttcggga gatatcctca
     4921 cataatccgc aggaacagaa acagtattgc aataattgac ggtacggaaa gcaaagggga
     4981 actccgggaa ctcggaaatg atgttttctc atatttcggg ctcgggtgca ggaatgtttc
     5041 actggtcttt ttgcctgaag gttatgatat cagagatata atatcggtat ggcatgaatt
     5101 ttcctcagcc gggaatcaca ataaatacgc cggcaactat gattttcaca aggcggtttt
     5161 tcttgtaaac agacaggaat tcacggatac cggatttctt ctcctgaagg aagataattc
     5221 gctgtcatct cccgtatctg ttctccatta ttccttctat aagaccgcag aggaactaca
     5281 gtcatggata agtacccgga aggacaaaat ccagtgcata acgggccacg gacatgtccc
     5341 ttttggaaga tcgcagatgc cagccctgtg ggattatgca gacggagtgg acacaattga
     5401 atttcttctt aaaaaaaaac agcgtggaat attgtaaagt aaaaaatact atattgcatc
     5461 atcacattaa caacgattat ggtatataaa tttgtcgttt tatcggatga ggatgagtca
     5521 tttatgagag aatttgaatt tcttgactca cagaccctgc tggattttca caatctgatc
     5581 caggaggaac ttgaatttga taaatcacag atggcgtcat tcttcatggc agcagacaat
     5641 tgggaaaaag aggaagaatt tacgctcttc gacatgggta cgggatcatc aacaatggag
     5701 gatactgtcc ttgaagatat tatttttcac aaaaatcaga agctgctcta tgtttttgat
     5761 tttttcaacg acagaagcct ttttatagaa tatacgggtg aagtcgagga aatcgacggg
     5821 cgcgaatatc cggtgtgttc aaattcaaaa ggcatacctc ccaaacaggt agtcttcgga
     5881 agcagttcaa agaagctata cagaaacatt attgtatccg acgacgatga cgatgatgat
     5941 gaacccgaca tggatgagct tttccttgac gatgacgatg atgtgctgcc ggacttcgat
     6001 aatgatgaac ccatgatcga cgacgaggac gacgactagg cgtcacggaa aaaacgcatg
     6061 aagaatatcc tgattgtcct gctcggccct accggtgtcg ggaagaccga tctttccatt
     6121 gaccttgccg tacatttccg ctgtgatata atttctgccg attcacggca gttctacagg
     6181 gaaatgaata tcggaacagc ggtcccttcc ccggatcagc tttcaagggt aaaacatcat
     6241 ttcataggat tcctttccgt aaaagactat tacagtgcaa gtctctatga gagagatgta
     6301 ctcaggctcc ttccctcttt gttcgccagg aatccgcttg tattaatgac aggaggctca
     6361 ggattataca ttgatgcggt ctgcaggggc atagatgaca tcccggatac cgatcaggcc
     6421 atcaggagga aatatgtaca gaaatacagg gaagaaggta ttgagagcct gagaatgctg
     6481 ctcaggatgc tcgatcccgt ctattatgaa aaggtggacc tgaaaaatcc ccggagaata
     6541 ataagggcac ttgagatttg tgaaagcact ggacgtccct attccgcttt tctgacaaaa
     6601 aaggaacagc cgagaaactt tgaggtgata cggataggca tcaaccggcc cagggaagaa
     6661 ctgttcggaa ggatcaacag gagggttgac gagatgattg cagcaggact ggaggatgaa
     6721 gctgaaaggc tgtattccct ccggggtatc aatgccctca acagcgttgg ttacacggag
     6781 ttatttgaat atttttcctc acgtataaca agggaacagg ccatagggct tataaagcgc
     6841 aattcgcggc gatatgcaaa acggcagctg acctggtgga acagggacag ggagattgcc
     6901 tggttcgggg ctgatgaaaa aaacaggata acagaatatc tcagcaataa aataaacagc
     6961 ctctctgaat aaaaacaatt tcctgaataa caggagacag caaggaacaa aatggcagtg
     7021 ctgcaaaacc ttcctgccgg aacagctcca ttatgtaacc ccgcgtatac agggtttccg
     7081 gaaccgtttt cattcttcaa gcatcttcag cttccggatt gtatccgcag gatccccgga
     7141 actgaaaaca gtgtttccgg caaccagcac atcggctcct gtctcaacga gttcagcagc
     7201 attgtgcgtg tcaatcccgc cgtccacctg aataagcaca tcatgccccc cggttctgat
     7261 catatattta agttcctcta tcttgccgta actctcctct atgaatacct gtccgccgaa
     7321 tcccggattc acggtcatta ttaatatcat atccacagta tgcagaatat ttttcagcag
     7381 cgccaccggg gtatggggat taacagcaac accggccttc atgccaagcc tgcggatctc
     7441 cgttactgtc cggtgaagat ggacacaggc ttcgtactga acggtaagaa tggcagctcc
     7501 cgtttccttc cagcggacaa gatacctgtc gggatccact atcatcagat gaacatcgag
     7561 cggcttttca gctactttcc ttacgtgctc aaccaccggg aaaccgaagg agatgttggg
     7621 aacaaaaaca ccgtccatta tgtcacagtg tatccagtcg gccatgcttc cgtttataag
     7681 ccggacatct tccgaaaggt ttccgaaatc ggccgccaga agggagggtg atacaagatg
     7741 attcatttca aatggttttt tttcaaatat atgtttttcc cggatatata tttttcccac
     7801 aaatatgctt ttagcggata aaaaatttca tgtataaatg atttcacggt aaatatagca
     7861 taatgaaaat tattgtgcgg caaagaattt agtctgccgg agaatctggg catgacgaaa
     7921 aatatggatc tgcttttaac ccaggtaggt acggaggatt ttacaccttg tggtaaactg
     7981 tattcttttg attgccttct cctttatctg cctgaccctt tcacgcgtca ggtgaagttt
     8041 ctccccgatt tcttccagcg taagaggatg tttcattcca agcccgaaat aatatttcag
     8101 gactttcgct tcccgggggg aaagagtgct caatgcacgt tctatctcgt atgaaagtga
     8161 ttcgtttatc agattcctgt caggacttgg tatttcatcg ttctggataa catcatacat
     8221 gttattatcc tcctccgcac tgatcggggc gtccatgctt accgtccgtc cgttggtcct
     8281 gagggattcc ctcacatcct caggaatcat ttccaggagt tcggctatct ccagggatgt
     8341 tggttcacgt tcaaattcct gttcaagcct gatcattgcc ttgtttatcc tgctgatcga
     8401 tccgattttg ttaaccggca gtctgactat tctcgactgt tctgccagtg cctggagaat
     8461 tgcctggcgg atccaccaca ctgcatatga aatgaatttg aatcccctag tctcgtcgaa
     8521 acgggatgca gccttcatca ggcccagatt cccttcattg atcaggtcgg gaaggctcat
     8581 tccctggttc tggtattgct ttgccacaga cacaacaaag cgcaggttgg aattgataag
     8641 ctttttcagg gcttcggtat cacctgccct tatcttcctg gccaggatta cttcctcttc
     8701 agggctgatc agttcaaccc ttccgatctc ctgcagatat ttgtccagag aaggtgtatc
     8761 acggtttgtg acctgcttgg ttattttcag ctgacgcatg acaggcccgg attagttaca
     8821 agggatcagg tactcaaaat acaattttaa taaaaaaact gatatccccg acagataatg
     8881 aatgtttttt ttgggaaatt catttttaag taataaattg cggtgctttt taagtgaaga
     8941 acaaagcact aactcaatta attattttcc gttgcatttt attttgttaa ctattagaat
     9001 tctgacattt ttattatact tgtaccggtt ttcaggacat gctatattag cgctgcatta
     9061 actgtctggc tcatagtcat tcttgatacg gttcccaaaa aacaaattca aattggtcat
     9121 tttttattat tccatcatta ttactgacat atgtacgaca ttcttgaatt aagtaaaaag
     9181 ctactgcccg aattacggga aatagcgaaa acacttaata ttaaaaaggc cgattcgctg
     9241 aaaaagcagg atcttgttta taaaattctt gatcagcagg caattacttc cacagaatca
     9301 ataaccattc agaaaaagga agacccgccc cagggtaaat ctgaaccgga acccgttcgc
     9361 gagaagaatc aaagacgggg gaaaaggccc agaaccgtta agcccttatt caagagcccc
     9421 acggaatccc aggctgcctc atctcaggga gccggcagga ccacggctgc cctggccgga
     9481 aatgaaatgg cagacgaaag acggtctcct gccatcaggc ctgaaccaaa atctgaggaa
     9541 aaggctgctg gttccggcaa acgcagaaac agtggtgctg atacagctgt aacagaagct
     9601 gttcccgaaa cagcagaggg taatacttcc gccggcgatg accaggctgc tgaaaaaacc
     9661 atgacaaccg gcacgccagc atctgaaccg gtaactgcag aagctgccgc ggcaccccgg
     9721 ccggcgcctg ccagggaaga aaagaaggaa aaaccctatg aatttgaagg tataatctat
     9781 aatacaggtg tgcttgagat catgcccgac ggttacggat ttctcagatc acccgattac
     9841 aactacctca attcaccgga tgatatatat gtgtcccagt cccagataaa acttttcgga
     9901 ctgaaaaccg gggatacaat aaagggaaca atcagacctc ccaaggaagg agaaaaatat
     9961 tttcctctta tcaaagtgga tgagataaac ggaagaagtc ctgattacat aagggacagg
    10021 gttcccttcg acttcctcac tccccttttc cccaatgaga aattcaccct gtgcaccccg
    10081 ggcgaaggaa gtctttccgt aagggtgatc gacatgttca caccgatagg aaaaggtcag
    10141 agaggactta tcgttgccca gccgaaaaca ggaaaaacca ttcttctcca ggaaatagcc
    10201 aatgccatag caaaatatca tcctgaagta tacctgatga tcctgctgat tgatgaaagg
    10261 cccgaggaag tgaccgacat ggcacgcaac gtaaaggctg aggttattgc atcaaccttt
    10321 gacgagcctg ccgaaaggca ctgccgcgtg gccaacatcg tactcgaaaa agccaaaagg
    10381 ctcgttgaat gcggacatga tgtcgtaata cttcttgact ccattaccag gcttgccagg
    10441 gcattcaaca ccactgcacc cgcatcaggc aaggtacttt cgggaggtgt cgattcaaat
    10501 gccctgcaca aaccgaaaag attcttcggc gctgcccgta acatagaaga cggcgggtca
    10561 ctgaccattc tggcaaccgc cctcacggaa accggatcca agatggatga tgtgattttc
    10621 gaggaattca agggaacagg aaacatggag cttcagctcg acaggaaact gtcaaacagg
    10681 aggatctttc cgtcaatcga catacccgct tcaagcacca gaagagaaga ccttctcctc
    10741 aataagaata tcatgcagaa aatctgggtg ctgcggaatt acctggccga catgaattcg
    10801 cttgaggcaa tggaattcct cagggacagg ctgatgcaga gcaggtcaaa tgaagaattc
    10861 ctgatctcta tgaatggtgg ataatccaaa taaaattcca tacatttgta gccttgttaa
    10921 aaaattaaca atcaaacata atttatttta aaaaaaatat gggaacagga aaatttataa
    10981 gatgcgacgg taaccaggcc gcatcgcaca ttgcatatat gttcagcgag gtcgcttgca
    11041 tatatcctat aacaccatct tccaccatgg ccgaatttgt tgatgaatgg tcggccaacg
    11101 gattgaagaa catgttcggg gaacaggtca aggttgttga aatgcaatct gaaggaggcg
    11161 ctgccggtgc agtgcatggt tcccttcagg cgggagctct ctgttcaaca ttcacggctt
    11221 cacagggact gctccttatg ataccgaaca tgtacaaaat tgccgcggaa cttcttcccg
    11281 gcgtgttcca tgtgagcgcc aggacaatcg ccgcacatgc actgtcaata ttcggagatc
    11341 acagtgatat ctatgccaca cgccagacag gcttcgcgat actggccacg ggaagcgtac
    11401 aggagataat ggatctcgga ggcgtagcac acctttccgc tataaagtca agaataccct
    11461 tcctgcattt ctttgacgga ttcagaacat catccgaagt acagaaggtc aggctcatgg
    11521 aaatggaaga cctgaagaaa ctggtagatt ttgaggcgct ggaaagattc cgcaacaatg
    11581 ctctcaatcc ggaacatccg gtaacaaggg gaacagccca gaatcctgat atcttcttcc
    11641 aggcaaaaga agccatcaac aggttttacg agccggttgc cgatatcgtt aacgattaca
    11701 tgactgaaat ttcaaagatc accggaaaag attacaggcc tttcacatat tacggagcac
    11761 ccgatgcaga aaatattatt atcgcaatgg gttccgtcac tgacacagcc aaggaagtaa
    11821 tagattatct ggcagaaaag ggtgaaaaag caggactgat ctccgtacac ctttaccggc
    11881 ctttctcagc gaaatacttc tttaacgttc ttccctcaac agtaaagaaa atcactgtcc
    11941 tcgacaggac aaaagaaccg ggggcaccgg gagaacctct ttatcttgat ataagggacc
    12001 tgttctacaa ccgttccgaa agacctctta ttatcggagg ccggtacgga ctaagttcaa
    12061 aggataccac acctgcacag gtgcttgccg tttatgaaaa catgaagctt gccgaaccca
    12121 aaaaccagtt caccgtaggt atagttgacg atgtaacaca tacatccctg cctctccttc
    12181 cggaaataaa cgttgctccc aaaggaacct actcggcaaa attctacggt ctgggttcag
    12241 acggaacagt tggcgcaaac aaaaactcaa tcaagattat cggcgacacc accgataaat
    12301 attgccaggc atactttgca tacgattcaa aaaaatcggg agggatcacc atttcccatc
    12361 tccgcttcgg ggataatccg ataagatcgc cctattatgt aaacacaccc gatttcgtgg
    12421 cctgccatgt cccctcctat ctcgataaat atgacatgct aaagggactg aaaaaaggag
    12481 gcacatttct ccttaactcc atatgggatg cggaagaaac caagaaacgc cttcctgacc
    12541 atatgaagaa atatctcgcc gtaaatgaaa tcaatttcca cataatcaat gcaaccctga
    12601 tagccgagga aatcggtctt ggaagcagga ccaacaccat catgcagtct gcattcttca
    12661 ggctggccaa tgttattccc tatgacaagg ccgaggaaga aatgaagaaa gccatttaca
    12721 gcagctatgg cagaagaggc gaagaagtgg tcaagatgaa ttatgaggcc gtagccaggg
    12781 gcagtcaggt ggaaaaggtt gaaatacccg ctgaatgggc tgacattgta attaaaaagg
    12841 ctgaggataa gcgcgatatc ccggaattca tccgcgattt tgccgagcct gtcaacatgc
    12901 tgaaaggtga tgaccttccg gtaagtgcat ttgtgggaag agaagacggt accttccctg
    12961 ccggcacaac agcctttgaa aagaggggta tcgccgtgaa cgtgcccgaa tggcaggcag
    13021 aggaatgcat tcagtgcaac cagtgttcat atgtctgccc gcatgcagcc atacgtccct
    13081 tcctgcttac ggaagaagaa gccgctaatg ctccggaagg aacaaaaacc cttcagggga
    13141 ttccaaaaac actgaatgag tacaggttca ggatacaatt aagtctttac gactgtacgg
    13201 gctgcggcaa ttgtgccgat gtctgtccgt cgaagaacaa ggctctgata atgaagcctt
    13261 ttgaaaccca gctggaagaa ggtccgcgct ggacatacat gcacgaaaaa gtgggttata
    13321 aggatcatgt tgtagacaag ttcctgaacg tcaagaacag tcagtttgca cagcccctgt
    13381 ttgaattctc cggtgcatgt gccggttgcg gtgaaacacc ctatattaaa accatcaccc
    13441 agctctttgg cgacaggatg ataattgcaa acgctacagg atgctcttca atatacggcg
    13501 gttcagcccc gaacactccc tataccgtta ataagcacgg tcatggccct gcatgggcaa
    13561 actctctctt cgaagacaac gcagagttc
//
LOCUS       DAOSPX010000053        13516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4644_length_13516_cov_4.053414, whole genome shotgun sequence.
ACCESSION   DAOSPX010000053 DAOSPX010000000
VERSION     DAOSPX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4644_length_13516_cov_4.053414"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2218
                     /locus_tag="PLZ75_04260"
     CDS             <1..2218
                     /locus_tag="PLZ75_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOS71644.1"
                     /translation="IGQEASAVGMAYHLDTDDFIFGSHRSHGEIIAKGLSAIHKLDEE
                     TSYSLMKNYFGGNALKVVEKGFQGEIRDLAVHFLLYGALAEIFARENGFNKGLGGSMH
                     AFFTPFGIYPNNAIVGGSGDISVGAALYKKINRKPGIVVCNIGDASSACGPVWEGICF
                     ATMDQYRTLWEGEYQGGLPLIFNFMNNLYGMGGQTMGETMGFGILARLGAGVNPEMMH
                     AERVDGYNPLAVIDAFRRKIQILREKKGPVLLDTLTYRISGHSPSDASSYRTKEEIDL
                     WQKEDSIITYGSLLVSSGITTQDVLDEIREDTSALMTHILELAVDDDISPRLDLDRDP
                     DLIGRMMFSGESRDRMEEGKPETLIPLDENPRVKSIRNKERYYLDKDGKPVSKAKLFQ
                     LRDAIFEAIIDRFYKDPSLVAWGEENRDWGGAFAVYRGLTEALPYHRLFNSPISEGAI
                     VGTAIGYGMCGGRVIAEIMYCDFLGRSGDEVFNQLPKWQAMSGNILKMPVVIRVSVGS
                     KYGAQHSQDWSSLTAHIPGLKVIFPATPYDARGLMNSALQGTDPVVFFESQGIYDIGE
                     MFHRPGVPEEYYEIPIGEPDIKRTGSDISILTIGATLYTAIKAADILSEKFGLSAEII
                     DARTLVPFNYTPVIESLKKTGKIVLASDASSRGSFLKDMAQTITELAFDYLDAPPVVV
                     GSRNWITPAFEMEEYFFPQPGWIIDAIHQKIMPLEGHNPQYDFSDEKTIALNRDGI"
     gene            2338..2976
                     /locus_tag="PLZ75_04265"
     CDS             2338..2976
                     /locus_tag="PLZ75_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOS71645.1"
                     /translation="MDEPEKYRNERKEVARFMRRLYRHGLTTTSGGNISRRLDDRLIA
                     ITPSATDKGRMKWKEVGIMSLTGENLTPHLRPSIEHSMHIKIYNSNKVVNAIIHAHPV
                     YATSFTAMKHAIDTSLTAEARAICGEPKLVPYALMGTEKLADTAAQHARDSDVMLLEN
                     HGVLTTAPTLLQAFDKLEVLENAAKMTLIVEISGGKSPLSKSRLTEIDRIFR"
     gene            2973..3617
                     /locus_tag="PLZ75_04270"
     CDS             2973..3617
                     /locus_tag="PLZ75_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOS71646.1"
                     /translation="MIKAVLFDMDGVLADSEQLISRAAAMMFGELGIKVEQHDFKPFT
                     GTGENRYIGGVAEKYNISIDIEKAKARTYEIYMKIIRGKLKPHPGAAALVSRCRETGL
                     KLALATSSDRIKTEATLAEIGISPGSFDTVITGSDVKNKKPFPDIYLEAARRTGYTPG
                     ECLVIEDAPSGIKAAKSAGCRCLAVTTTFEKSLLAGADWICDTLENIPEEALKW"
     gene            3640..4908
                     /locus_tag="PLZ75_04275"
     CDS             3640..4908
                     /locus_tag="PLZ75_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOS71647.1"
                     /translation="MKKNFLENIPWHDELTKKNADIELPDYREANAHIHTPYSFSSFD
                     SMDTLFRQAREEEIAVLGINDFFVADGYETFHNGCVRNRIFPLFNIEFIGLLKEEQKK
                     GIRINDPNNPGRIYLCGKGLDYPFSLNWWLRRKLKNVIRENQNQIKAMIDRLNRLIEQ
                     VNPALVLSYGEIRTRFAKNLVRERHLAKALRSLAVDNYEKAGDQVEFIESLYNGKKVK
                     AAISDQAAMENEIRSNLLKAGGPAFVGENEKSFPSLEMLIRIIIKAGGIPCYPVLLDD
                     ASGKLTEFEADMEKLYSSLSSAGIGCIELIPSRNDPSMLENCTEYFNEKGFIVTFGTE
                     HNTPDLAPLAITSRGSIPLKENLRKTAWEGACVIAAHQYLRTHGRQGYVLDDGTLCVD
                     QKNDLAALGRNVIEYFLNNSQHESGNKGVN"
     gene            4880..6853
                     /locus_tag="PLZ75_04280"
     CDS             4880..6853
                     /locus_tag="PLZ75_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS71648.1"
                     /translation="MKAEIKELIEISRFYGGNKEYVIAGGGNTSFKDENTIWVKASGQ
                     SLAELTEEGLVALSRNKLREISGKTYADDPMLREEQVKNDLYACMQNPLEGKRPSVET
                     SLHELISYSFVVHLHPTVLNGILCSRNAGKTVASLFGDKALFVPYTDPGYTLFKALET
                     EITAYRKLHQRDPKCIFLENHGVFAAADTTAEIKDIYRDLIGSARKNLPEPVKAEDLP
                     FNQVMHRVLPVIRMMLSEDRPKIIRGRHNTLIAEYYGSQQDFYKVSLPLTPDMIVYCK
                     TKYLYIEQSASAERIIESLRMQLPRFRTEYGYLPKLIIIKNAGVFAVEDTPAAAETTL
                     DVYEDLMQVSYYASGSGGIKHLTAEQIAFIDQWEVENYRRKVAAANLSSDKLSGKIAI
                     VTGGARGFGAGIAESLHRLNLNVVIADLNEETGTGLVSELSQKPSSGKIIFKKTDVAD
                     PESVKELVAFAVSEFGGLDLMISNAGVLRAGGLEEMEAEVFSMTTNINYNAFFYCAKY
                     ASAVMKLQNSGKPGYFTDIVQINSKSGLRGSNRNFTYAGAKFGGIGLTQSFALELAPY
                     RIKVNAICPGNYYEGPLWSDPENGLFVQYLKTGKVPGAKTIEDVRKFYEDQVPLKRGC
                     RLEDLVRALLYVVDQEYETGQAVPVTGGQVMLG"
     gene            6955..8223
                     /locus_tag="PLZ75_04285"
     CDS             6955..8223
                     /locus_tag="PLZ75_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOS71649.1"
                     /translation="MKTKAVRIYGKNDLRLEEFDLPPMKDDEILAQVVSDSICMSTHK
                     AALQGAAHKRIPDDIHVNPTIVGHEFSGIILEVGKKWQHKFSRGQKFSIQPAMSHTGT
                     LDAPGYSFRYIGGNATHIIIPDIVMEADCLLPYDGEAFFPASLSEPMSCIVGAFHASY
                     HTRPGTYIHEMGIRRGGKMAILAGVGPMGLGAIDYALHQERKPSLLVVTDIDDSRLAR
                     AACLFTPEHAAEEGVKLVYVNTAKTDDPVSYLRELSGGNGYDDVMVFAPVKQVVEQGD
                     AILGFDGCLNFFAGPTNPDFKAEFNFYNVHYAFTHIVGTSGGNTDDMRESLRLMGEGR
                     INPAVMITHIGGLDAVIDTTLNLPGIPGGKKLIYTNISMPLTSLYKLGEKGEKENNRL
                     YRELHEIVSKNDFIWSLEAEKYLLANADPI"
     gene            complement(8298..9224)
                     /locus_tag="PLZ75_04290"
     CDS             complement(8298..9224)
                     /locus_tag="PLZ75_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS71650.1"
                     /translation="MRQIFTIGESLYDIIFKDGRPHEGKPGGAMLNSSVSLGRAGLPV
                     SFISEYADDETGNLIDAFLVDNGVGNKFIRRYSDGKTALAIAVLNEKNDASYTFYKDY
                     PEHRLQMEFPRVQADDIVLFGSIYAITKEIRARLVQFISTSAKNGAIVIYDPNFRTAH
                     APGLARLKPLILENMQMSAIVRGSDEDFRNIFQAESPDSAWSVVGNYSPCLVYTASAD
                     AVYVRTAGFSGMFPVRKITPVSTIGAGDNFNAGMIAAIYRAGIKKDELEMMGEEVWHS
                     VITAGMDFATDVCMSYENYISRELAGKYRGGT"
     gene            9293..10327
                     /locus_tag="PLZ75_04295"
     CDS             9293..10327
                     /locus_tag="PLZ75_04295"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOS71651.1"
                     /translation="MKAMMLTGIRQMEMREVNDPVIKQPDDVRIRMLAVGVCGSDIHY
                     YTQGRIGSQEVKYPFTVGHEGSGIIVETGPAVTRLKAGDRIAIEPSMPCGSCDQCLSG
                     RSHTCRRIKFLGCPGQAEGALSEFIVMPEANCLVLPDNLNSDHGSISEPLAIGVYSVK
                     KSGGVKGLRIGILGFGPIGMSVMLAARADGAEKIYVTDKIDERLEIALKEMVCSASNP
                     LRDDIVKIIRDHEPPGLDVVFECCGQQDALDQAVDLLKPGGKIIVTGIPEFDNWSLNV
                     ENTRRRELSVQFIRRQVDCAETAIVMMSKGTIDIGNMVTHRFPFERTREAFDLVAGYR
                     DGVMKAMIDF"
     gene            complement(10324..11403)
                     /locus_tag="PLZ75_04300"
     CDS             complement(10324..11403)
                     /locus_tag="PLZ75_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOS71652.1"
                     /translation="MEQYTLMYNVMKGIPGRLPRTIDAAGSLVTAEPDWWTSGFFPGT
                     LWYLYEYSGDVKFREYAEEMTARIEKEKDNRGTHDLGFMLYCSFGNGLRLTGNEAYKD
                     ILLTGAKSLITRYRPNIGCIQSWNSRRGWQCPVIIDNMMNLEFLMWAFRVSGDSTYYR
                     ICVNHSDTTMKNHFRPDYSSWHVVSYDTISGRVEKKQTAQGYSDASAWSRGQCWGLYG
                     YVVMYRETGLQKYLDQATGIAEFLINHPRMPEDGIPYWDFDDPAIPDTERDASAGAIM
                     ASALIELSGMTAEPASSRYLNMAELQLRSLASPRYRAGKGENGNFILMHSTGSLPDDS
                     EVNVPLTYADYYFIEALLRYRALKD"
     gene            11607..11978
                     /locus_tag="PLZ75_04305"
     CDS             11607..11978
                     /locus_tag="PLZ75_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOS71653.1"
                     /translation="MATSKKLLSVTNMPVFKEKLTPAERISPDFERRIAFLNKLMIVV
                     CDFTNGPAAEPEKPHSHPHEQISYVAEGELYLFLGEEKHRLSKGDIFTVPSGIPHCIQ
                     TLSSHVRLIDAFSPVREDYIG"
     gene            12044..13192
                     /locus_tag="PLZ75_04310"
     CDS             12044..13192
                     /locus_tag="PLZ75_04310"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOS71654.1"
                     /translation="MATKKLKSGIKGEKRKTANTISALLIICGMFAMAAMAPGHREYA
                     GKDAGAEISLMQDAPKSVKNISFKKNFTAGNFTSVLVDDNNTKWFLTDKGIASFDGSK
                     WQIHNKNKQIPVTGVKDFAIDVSSAGRELWLASPAGATAVKLPITAKSAITTYNTGNS
                     ELLSDTVRAVAVGKGSLRWFGTNRGVSAYINGKWLSYDYELFYPEVIFRDFPITAIAT
                     NPGGDSVYVATDGAGVGRMFRNEVDAISGASEYAIWGPIDMPSDNVHSICITPDGTKW
                     FGTDLGIARHIGNETLAEWTVFDTNDGLVDNFVQCIACYPDGSVWFGTKGGISIFRDD
                     SWKSLTSENGLLSNNIQCMTVDKTGVVWCGTDKGVIAIDRGVITNYTH"
     gene            complement(13270..13425)
                     /locus_tag="PLZ75_04315"
     CDS             complement(13270..13425)
                     /locus_tag="PLZ75_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71655.1"
                     /translation="MGILYFRRAAGQEIFLIRGRSGRGVGDFSSERNHITWMDVASRL
                     KIGIGYF"
BASE COUNT         3940 a         3171 c         3234 g         3171 t
ORIGIN      
        1 catagggcag gaagcttcag ccgtaggaat ggcataccat cttgacactg atgatttcat
       61 cttcggatca caccggagcc acggggaaat tatagcaaag ggactatcag caattcataa
      121 acttgacgaa gagaccagct attcactgat gaagaattat ttcggcggaa atgcccttaa
      181 agttgtggaa aagggatttc agggggaaat acgggatctg gctgttcatt ttcttctgta
      241 tggtgccctt gctgagatct ttgcgcgtga aaacggtttt aacaaaggac tgggaggatc
      301 aatgcatgct ttcttcacac ctttcggcat ttatcccaac aacgcaatag tcggaggttc
      361 gggtgatatt tcggtaggag cggcattgta caaaaaaatc aacagaaaac cgggcattgt
      421 tgtctgtaac ataggcgatg catcctctgc atgcggcccc gtatgggaag gaatatgctt
      481 cgccaccatg gatcagtaca ggacgctctg ggagggagaa taccagggag gactgccgct
      541 gatattcaat ttcatgaata atctctacgg catgggcggc cagacaatgg gggaaaccat
      601 gggattcgga atcctggccc gcctgggagc aggagtgaat cccgaaatga tgcatgccga
      661 acgtgtggac ggctataatc cccttgcagt aatagatgcc ttcagacgaa agatacagat
      721 actccgggaa aagaaaggac ctgtactcct cgacaccctc acctacagaa taagcggaca
      781 ctctccctcg gacgcctcgt cctacaggac aaaggaggaa atagacctat ggcagaagga
      841 ggattcaata atcacttacg gcagtctgct ggtaagctca ggaatcacta ctcaggacgt
      901 gctcgacgaa ataagggagg acacatctgc actgatgaca catattcttg aacttgcagt
      961 tgatgatgat atttcaccca gacttgacct tgaccgcgat cccgatctga tcggacgaat
     1021 gatgttttcc ggcgaatccc gcgacagaat ggaggaagga aaaccggaaa ccctgatacc
     1081 tcttgatgaa aatccccggg taaaatccat caggaacaag gaaagatatt atctggacaa
     1141 ggacggaaaa cccgtgtcaa aagcaaaact gttccagctg cgcgacgcga tcttcgaagc
     1201 cataatagac cgtttctata aagatccttc acttgttgca tggggcgagg aaaacaggga
     1261 ctggggagga gcatttgctg tttacagggg acttaccgaa gccctgccct accaccggct
     1321 cttcaactcc ccgatttccg agggggcgat tgtaggaacg gcaataggct acggaatgtg
     1381 cgggggaagg gtgattgccg aaataatgta ctgcgacttt ctcggacgat ctggtgatga
     1441 agtgtttaac cagctgccga aatggcaggc catgagcgga aatatattaa aaatgcctgt
     1501 ggtaataaga gtgtctgtcg gctcgaaata cggtgcacag cattcccagg actggtcatc
     1561 gctgacagcc catatcccgg gcctgaaagt tattttcccg gccactccct atgatgccag
     1621 gggactgatg aattccgcac tgcagggaac cgatccggta gtcttcttcg agagccaggg
     1681 tatatatgat atcggtgaaa tgtttcaccg gccgggtgtt cccgaagaat actacgaaat
     1741 acccataggc gaacccgaca tcaagaggac gggaagcgac atcagcatac tgaccatagg
     1801 tgcaacgctc tacacagcca taaaggcagc agatatactg agcgaaaaat tcggcctttc
     1861 agctgagata attgatgcca gaaccctcgt tcccttcaat tatacacctg taattgaatc
     1921 acttaaaaag acaggaaaga tcgttcttgc aagcgatgcc tcctcccggg gatcctttct
     1981 gaaggatatg gcccagacca ttacggaact ggcttttgat tacctggatg ctccccccgt
     2041 ggtggtggga tccaggaact ggataacacc ggcattcgaa atggaagaat atttctttcc
     2101 gcagcccgga tggataatag atgcaattca tcagaaaatc atgccgctgg aaggacacaa
     2161 cccgcagtat gatttttcgg acgaaaaaac cattgccctc aacagggacg gaatatagcc
     2221 cccggaacca ggagatgaac aggaagtaaa tttccaactt cctgccatcc ctgctacttt
     2281 ttaatatctt taccggcata aaaaccattt cctgacttaa ggcaaacatg ttgaccgatg
     2341 gacgaaccgg aaaaatacag gaatgaaaga aaggaagtag cccgtttcat gagaagactc
     2401 taccggcacg ggctgacaac aacctcggga ggaaacatca gcaggagact tgacgacaga
     2461 ctcattgcaa taactccctc cgcaactgac aagggaagaa tgaaatggaa ggaagtggga
     2521 ataatgtcac tcaccggtga gaatctgact cctcatctca gaccttcaat tgaacacagc
     2581 atgcacataa aaatatacaa cagcaacaag gttgtaaatg caataatcca tgcccacccg
     2641 gtttatgcca cttcattcac tgccatgaaa cacgccatag acacatccct taccgctgaa
     2701 gcccgggcca tatgcggtga accgaaactg gtgccctatg cgctgatggg cacggagaaa
     2761 ctggcagaca ctgcagcaca acatgcgagg gattcggatg tgatgcttct ggaaaaccac
     2821 ggcgtcttaa ctaccgcgcc gacacttctc caggctttcg acaagcttga agtgcttgag
     2881 aatgcggcta agatgactct gattgtcgaa ataagcgggg gaaaaagccc gctcagcaaa
     2941 tcccggctga cggaaattga cagaatattc agatgataaa ggctgtgctt tttgatatgg
     3001 atggcgtact tgccgactcg gaacagctga tcagcagggc tgcagccatg atgttcggag
     3061 aactgggaat caaggttgaa cagcatgatt tcaagccttt caccggaaca ggggaaaatc
     3121 gctatatcgg aggtgtggca gaaaaataca atatcagcat cgatattgaa aaagcaaagg
     3181 cccggaccta tgagatttat atgaagataa tcaggggaaa actcaaacct catcccggag
     3241 cagccgcact tgtcagccgg tgcagggaga cagggctgaa actggctctg gccacaagct
     3301 ccgacaggat aaagacggaa gcaaccctgg ctgagatagg aatttctccg ggttcctttg
     3361 atacggtaat aacaggatcg gatgtgaaaa acaagaagcc cttccccgac atttatcttg
     3421 aagctgcccg gagaacggga tatactcccg gggaatgcct tgtcatcgaa gatgccccca
     3481 gcgggataaa ggctgccaaa agtgcaggat gccgctgcct tgcggtaaca acaacatttg
     3541 aaaaatcatt gcttgccggg gcggactgga tctgtgacac tctggaaaac atcccggagg
     3601 aagcattgaa atggtaatat caacataaaa tcatttgctg tgaaaaaaaa tttccttgaa
     3661 aatataccct ggcatgatga actgacgaaa aagaacgccg atattgaatt accggattac
     3721 agggaggcaa atgcccatat tcacactccc tattctttca gttcctttga ttcaatggac
     3781 acgcttttca ggcaggcccg ggaggaggaa atcgccgtcc tgggcatcaa cgattttttt
     3841 gttgccgatg gttatgagac ctttcataac ggctgtgtca gaaacaggat attcccgctt
     3901 ttcaacattg agttcatcgg acttctgaaa gaagaacaaa agaaaggcat aagaataaac
     3961 gatcccaata atcccggcag gatatattta tgcgggaaag gccttgatta tcccttttcc
     4021 ctgaactggt ggctcagaag aaaactgaag aatgtcatcc gggagaatca gaatcagata
     4081 aaggccatga tcgacaggct caacaggctg attgagcagg taaacccggc acttgttttg
     4141 tcctacgggg aaatccgcac gagatttgca aaaaaccttg tgagggaacg ccatctggca
     4201 aaggccctca gatccctggc agtcgacaat tacgaaaagg caggggatca ggtggaattc
     4261 attgaatcac tctacaacgg caaaaaggtg aaagccgcca tttcagatca ggcagcaatg
     4321 gaaaacgaaa tcagatcaaa ccttcttaaa gcgggagggc ctgcctttgt cggggaaaac
     4381 gaaaaatcat tcccatctct tgaaatgctg atcaggataa taataaaagc cggcgggatc
     4441 ccgtgctacc cggtactgct ggatgacgca tcgggtaagc tgacggaatt tgaagccgat
     4501 atggaaaaac tttacagctc tctttcatct gcaggaatag gctgtataga gcttattccc
     4561 tccaggaatg atccttcaat gctcgaaaac tgcacagaat attttaatga aaaaggcttt
     4621 atagtcactt tcggcacgga acacaatacc ccggaccttg caccgcttgc aattacatcc
     4681 aggggcagca ttccgctgaa agaaaacctg aggaaaactg cctgggaagg cgcatgcgta
     4741 atagcagcac atcaatatct gagaacacat ggcaggcagg gctacgtact tgacgacggc
     4801 acactgtgcg ttgaccagaa aaatgacctg gcagccctgg gaagaaatgt aattgaatat
     4861 tttctaaaca acagtcagca tgaaagcgga aataaaggag ttaattgaga tttcgcgatt
     4921 ttatggcgga aataaggaat atgtgattgc cggcggcgga aatacttctt tcaaggatga
     4981 aaatacgata tgggtaaagg ccagcggcca gtcccttgcg gaactgactg aagagggcct
     5041 ggtggccctg agcaggaata aattaaggga aatttcggga aaaacatatg cagatgaccc
     5101 gatgctcagg gaggaacagg taaaaaatga tctttacgca tgcatgcaga acccgctgga
     5161 aggcaaaaga ccatcggttg aaacatctct tcatgaactg ataagctata gcttcgtggt
     5221 gcacctccat cccaccgttc tgaacgggat actctgcagc cggaacgcag gaaaaactgt
     5281 cgcaagccta tttggagaca aggccctgtt tgtaccgtat acagatcccg gctacacact
     5341 ttttaaagcg cttgagacgg aaattaccgc atacaggaag ctgcatcaga gggatcctaa
     5401 atgcatattc cttgaaaacc acggtgtttt tgcagcagca gatacaaccg cggaaataaa
     5461 ggacatctac agggatctta tcggatcagc ccggaaaaat ctgcccgaac ctgtaaaggc
     5521 agaagacctc cccttcaacc aggtaatgca cagggttctc cccgttatca gaatgatgct
     5581 ctcggaagac aggccaaaaa taatacgcgg caggcacaat acccttatcg ctgaatatta
     5641 tggttcccag caggatttct ataaggtatc gctgccgctc acgcccgaca tgatagtata
     5701 ctgcaaaaca aagtatctct atatagaaca atctgcaagt gccgaaagaa tcattgagtc
     5761 attgagaatg cagctgccga ggttcaggac tgaatacggc taccttccga aactgatcat
     5821 tattaaaaat gcgggtgtat ttgccgtgga agatactccg gctgcggcag aaacaacact
     5881 cgatgtttac gaggacctta tgcaggtaag ctattatgcc tccggatcag gcggaataaa
     5941 acacctgact gccgaacaga tagcattcat tgaccagtgg gaagttgaaa actacagacg
     6001 aaaagtagct gccgcaaatc tatcctcaga caaactctca ggtaaaatag caatagttac
     6061 cggtggtgcc cgggggtttg gtgcaggcat agccgaatcg ctgcacaggc tgaaccttaa
     6121 cgtggttatc gcagacctta atgaagagac aggaacagga ctggtatcag aattatcaca
     6181 aaagccttct tccggaaaaa taatattcaa aaaaactgat gttgctgatc cggaatcagt
     6241 taaggaactt gtagcctttg ctgtaagtga attcggcgga cttgatctca tgatcagcaa
     6301 tgccggggta ctcagggccg gaggacttga ggaaatggaa gcggaagtgt tctccatgac
     6361 aacaaatatc aactataacg cttttttcta ttgcgcaaaa tatgcttccg cggtaatgaa
     6421 gcttcagaac agtggaaaac ccggctattt caccgatatt gtccagatca attccaaatc
     6481 cggacttcgg ggaagtaacc ggaatttcac atatgcaggt gccaaattcg gaggaatcgg
     6541 actgacccag tcctttgcgc tcgagcttgc tccatacagg ataaaggtta atgccatctg
     6601 ccccgggaat tattacgaag gccccttgtg gtccgatccg gaaaacggac tttttgtcca
     6661 gtatcttaaa accggcaagg tgccgggtgc aaaaaccatt gaggatgtta ggaaattcta
     6721 tgaagaccag gttcccctga aacgcggctg cagactggaa gacctcgtaa gggcactgct
     6781 ttacgttgtg gatcaggaat atgaaacggg gcaggctgtg ccggtaacag gaggccaggt
     6841 aatgcttgga taggacctgt cccggttaag ctgtaatacc ggaaagcctg aaaacatttt
     6901 agccctgaaa cagttaaaac tgcaggaacg gaaaaaagaa aaaacaatat caagatgaaa
     6961 acaaaagcag ttaggattta cggcaaaaac gatcttcgcc ttgaggaatt cgatctgccg
     7021 ccgatgaagg atgacgagat cctggcacag gttgtctccg acagtatatg catgtcaacc
     7081 cataaggcag cactccaggg agccgcccac aagcgcatac ctgatgacat tcatgtcaac
     7141 cccacaatcg tcggccacga attttcaggg ataatccttg aagtgggtaa aaagtggcag
     7201 cataagtttt cccgaggcca gaaattttcc attcagcctg caatgagcca tacaggaaca
     7261 ctggatgccc cgggatactc cttccgctac atcggaggca atgccaccca tataatcatt
     7321 ccggatattg taatggaggc tgactgccta ctgccttatg acggtgaggc attctttccg
     7381 gcttcgcttt cagaacccat gtcatgcatt gtaggcgcct tccatgcctc ctatcatacc
     7441 aggcccggaa cctatatcca tgaaatgggt atccgcagag gcggtaaaat ggcaattctt
     7501 gccggtgtcg gcccaatggg cctgggagct attgactatg cgcttcacca ggagaggaaa
     7561 ccctccctgc ttgtcgttac tgatatagat gattcccgcc ttgccagggc agcatgcctt
     7621 ttcactcccg aacatgccgc tgaggaaggt gttaaactgg tgtatgtcaa taccgcaaaa
     7681 acagatgacc ctgtaagcta tctgagggaa ctatccgggg gaaacggata tgacgacgta
     7741 atggttttcg cacctgtgaa gcaggttgtg gaacagggtg atgccatcct tggatttgac
     7801 ggctgcctga acttttttgc cggacctacg aatcctgact tcaaggctga attcaatttc
     7861 tacaatgtcc actatgcatt cacacatatt gtgggcacct cgggcgggaa taccgacgac
     7921 atgcgggaat ccctcaggct tatgggggaa ggcagaatca atcccgccgt tatgataaca
     7981 catatcggag gactcgatgc cgtaattgat accactctta acctgcccgg catacccggg
     8041 ggtaaaaaac tgatatatac aaacatttca atgcccctga cctcgttgta taaactcggg
     8101 gaaaaaggtg aaaaggaaaa taacagatta tacagggaac tgcatgaaat cgtttcaaaa
     8161 aatgatttta tctggtcgct ggaagctgaa aaataccttc ttgccaatgc agacccgatc
     8221 tgataaacgg accgggatat aataaacgga ccggcatgtc ccggcgcccc cgggcagagt
     8281 atagcatagt acaaaaatca ggttcctccc ctgtatttac cggcgagttc ccgggatata
     8341 tagttctcgt aactcataca gacatcagtg gcaaaatcca tacctgcagt aattacggaa
     8401 tgccatacct cctcccccat catttcaagt tcatcctttt tgatgcccgc cctgtatatg
     8461 gctgcaatca tgcctgcatt aaaattatca cccgcaccaa ttgtacttac cggtgtgatc
     8521 ttcctcaccg gaaacattcc cgaaaaaccc gctgttctta cataaacggc atctgcactt
     8581 gcagtatata ccagacaggg actgtaattg ccgacaacag accaggcact gtcaggagat
     8641 tctgcctgaa atatgttcct gaaatcctca tccgaccccc ttactatggc tgacatctgc
     8701 atattttcca gtatcagcgg ctttagccgg gccagtccgg gggcatgggc tgttctgaag
     8761 ttcggatcat aaatcacaat agcaccattt tttgccgaag tgctgataaa ctgcacaagc
     8821 ctggccctta tctcctttgt gatggcatag attgatccga acagcacaat gtcatccgcc
     8881 tgtacccgcg gaaactccat ctgcagcctg tgttcagggt agtctttgta aaaagtatag
     8941 ctggcatcat tcttctcatt cagaacagct attgccagag cggttttccc atcactgtac
     9001 ctccgtatga atttatttcc gacgccattg tcaacaagaa aggcatctat cagattaccg
     9061 gtttcatcat ctgcatattc gctgatgaat gataccggca gcccggcacg gccgagcgaa
     9121 acggaactgt tcagcattgc accgccgggc ttcccctcat gcgggcgtcc gtccttaaaa
     9181 attatatcat aaagagattc gccaattgta aaaatctgtc tcatatcctg aaaagtctgt
     9241 ctgataaagg aagttgatta tatttgtttt ccgaatttaa aaaatatttg caatgaaggc
     9301 aatgatgctt accggaataa ggcagatgga aatgagggaa gtaaatgatc ctgtcattaa
     9361 acagcctgac gacgtcagga taaggatgct ggcagttggt gtgtgcggct ctgacattca
     9421 ttattacaca cagggacgga tcggttcaca ggaagtgaaa tatcctttca cagtaggaca
     9481 cgaaggatcc ggaataattg ttgaaaccgg gcctgcagtc acaaggctga aagccggcga
     9541 caggatagca attgaaccgt caatgccatg cggcagctgt gaccagtgcc tgtcaggccg
     9601 cagtcatacc tgccgcagga taaagttcct cggatgcccg ggacaggcgg agggcgctct
     9661 ttcggaattc atagtgatgc ccgaggccaa ttgcctcgta ctgccggata acctcaattc
     9721 agatcacggc agtatctcgg agccactggc cattggcgtt tattctgtaa aaaaatcagg
     9781 tggtgtaaaa ggactgagaa taggtatact ggggttcggt cctatcggaa tgagtgtcat
     9841 gctggcagcc agggcagatg gtgcggaaaa aatatatgtc actgacaaga ttgatgaacg
     9901 ccttgaaata gcattgaagg aaatggtatg ctccgcctca aatccgctca gggacgatat
     9961 cgtgaaaata atccgggatc atgaaccccc gggacttgat gtggtattcg aatgctgtgg
    10021 tcaacaagat gctctcgatc aggcagttga ccttctcaag ccgggaggaa aaatcatagt
    10081 gacaggtatt cccgaattcg acaactggtc cctgaacgtt gaaaacacca ggcggcggga
    10141 attatcggtc cagttcataa gaaggcaggt ggattgtgcc gaaacagcca tcgtaatgat
    10201 gagtaagggc accattgaca tcggcaatat ggtcactcac cgtttcccct ttgaaagaac
    10261 cagggaagca tttgatcttg tagccggcta cagggatggc gtaatgaaag ccatgattga
    10321 cttttagtcc ttcagggccc tgtacctgag aagggcctca atgaaatagt aatctgcata
    10381 tgtcagcggc acattcactt cactgtcatc tggaagagac cctgtgctgt gcatcaggat
    10441 aaaattgccg ttttcaccct ttcctgccct gtaacgggga gaagcaaggg agcgcagctg
    10501 cagttcagcc atgttcagat atcttgaaga tgcaggttct gcagtcattc cgctcagctc
    10561 gatcagggcc gatgccatta ttgctccggc agaagcatcc ctttccgtat caggaatggc
    10621 cggatcatcg aaatcccagt aaggaatgcc atcctccggc atcctgggat gatttatcag
    10681 aaattccgca attcctgttg cctggtcaag atatttctgc agacccgttt cacggtacat
    10741 cacaacatag ccgtaaagtc cccagcattg ccccctcgac catgcggatg catcggaata
    10801 gccctgtgca gtctgttttt tctcaaccct gccgcttatg gtatcatagg aaacaacatg
    10861 ccacgagctg taatccggcc tgaaatgatt cttcatcgtt gtatccgaat gattcacgca
    10921 gatcctgtaa taagtggaat cacctgaaac cctgaaagcc cacatgagaa attccagatt
    10981 catcatattg tcaattatca ccgggcactg ccatcccctc ctgctgttcc aggactgtat
    11041 gcagccaata ttcgggcgat atcttgtaat cagcgatttt gcccctgtca gaagaatatc
    11101 cttataggct tcattgccgg tcagccttaa tccgttgccg aaactgcagt aaagcatgaa
    11161 gccaagatca tgtgtacccc tgttgtcctt ctccttttca attctggctg tcatctcctc
    11221 agcatattcc ctgaatttca catcacctga atattcataa aggtaccaga gtgttccggg
    11281 gaaaaaacca cttgtccacc agtccggctc tgccgtcaca agagaacctg cggcatcaat
    11341 ggttctgggc agcctccccg gtattccttt catcacattg tacataagcg tgtactgttc
    11401 catggaaaaa ttcatggatt ccctgatgat cccgtccata ggcgcaggcc tgttacagga
    11461 tgacacagct atcagaaagc ctgccaggat aaataatctt gttttcataa gcttaaaatt
    11521 tgggtaattt ctgaaaagta cttgtcagga tggaatgata tgatttatta atcaaatata
    11581 aaattttaaa gcgaaattta atatatttgg caacttcgaa aaaactgtta tctgtaacca
    11641 atatgccggt attcaaggaa aaacttacac ccgcggaaag gatctctcct gattttgaaa
    11701 ggagaatcgc atttctgaat aaactgatga tcgttgtatg tgattttaca aacgggccgg
    11761 ctgctgaacc agaaaaacct cacagtcacc cacatgaaca gatctcatat gtcgcggagg
    11821 gagagcttta cctctttttg ggcgaagaaa aacaccgcct ctcaaaaggg gatattttta
    11881 ctgtcccctc cggaataccg cactgtatcc agacactgag cagtcatgta agactgatag
    11941 atgcattcag cccggtaagg gaagactaca tcggatagca gagcggaatc agtgttctgc
    12001 tgaaaatgta ttaaaaattg ttaaatatta aaacaggatt caaatggcta caaaaaaatt
    12061 aaaatcggga atcaaagggg aaaaacgaaa aacagcaaat actatatctg ctcttttaat
    12121 tatctgcgga atgtttgcaa tggcggctat ggctcccgga cacagggaat atgcaggaaa
    12181 ggatgcaggg gcggaaatct ccctgatgca ggatgctccc aaatcagtaa aaaacatttc
    12241 attcaagaag aattttactg ccgggaactt cacttcggta ctggttgatg acaacaatac
    12301 caaatggttt ctcactgaca agggaatagc cagttttgac ggcagcaaat ggcagataca
    12361 caacaaaaac aaacaaatcc cggtaaccgg agtgaaagac tttgccattg atgtttcatc
    12421 cgccggcagg gaactgtggc ttgcatcccc cgccggggca acggctgtga aacttccgat
    12481 tactgccaaa tcagccatta ctacatataa tacaggaaac tcagagcttc tcagcgatac
    12541 ggtacgcgca gtggccgtcg gaaaaggatc gctcagatgg tttggaacaa acaggggagt
    12601 ttcagcttat atcaatggca aatggcttag ctatgactat gaattattct atcctgaagt
    12661 aattttccgc gatttcccca taactgcaat tgcaacaaac ccgggaggtg attccgtcta
    12721 tgttgcaaca gacggtgcag gtgtgggcag gatgttcaga aatgaagttg atgccatctc
    12781 cggtgcttcg gaatacgcca tctggggacc gattgacatg ccgtccgaca atgttcacag
    12841 tatatgcata acccccgatg gaaccaagtg gtttggaact gatctgggga ttgcccgtca
    12901 tataggaaac gaaactctcg ccgaatggac ggtttttgat accaacgacg gacttgttga
    12961 taattttgta caatgtattg catgctatcc cgacggctca gtatggttcg gaacaaaagg
    13021 cggcatctca attttcagag atgacagctg gaaatcactg acttcggaaa atggtcttct
    13081 cagcaataat atccagtgta tgaccgtcga taagacagga gttgtctggt gcggtaccga
    13141 caagggcgtg attgccatcg acaggggagt tataacaaat tacacgcact gagcggcttt
    13201 aacagaataa acctgccatg gtgcttttgc cagggctccc aaaagccgga atgcatcagg
    13261 caggtttttt taaaaatacc ctataccaat cttcagacgg gaagccacat ccatccaggt
    13321 gatgtggttt ctctctgaag aaaaatcccc gactcctctc ccgctccttc cccggatcag
    13381 gaaaatctcc tgcccggcag cccttctgaa gtaaaggatc cccatcactc tgatctttca
    13441 gtattatcat aagatttata atataaatta gtacttttat tctggtttag gatggccccg
    13501 tcatggaact ttagcc
//
LOCUS       DAOSPX010000054        13171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4758_length_13171_cov_4.743138, whole genome shotgun sequence.
ACCESSION   DAOSPX010000054 DAOSPX010000000
VERSION     DAOSPX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13171
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4758_length_13171_cov_4.743138"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /gene="carB"
                     /locus_tag="PLZ75_04320"
     CDS             <1..1820
                     /gene="carB"
                     /locus_tag="PLZ75_04320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOS71656.1"
                     /translation="KELVDEEEAILGYRGSMPPDDVLASAKRNGFADKYLSQLLGIPE
                     KDIRTKRVSLGIREAWEPVPVSGVEDEAYYFSTYNGPDKVGITDRKKIMVLGGGPNRI
                     GQGIEFDYCCVHAAFAIRDAGYESIMVNCNPETVSTDYDTADRLYFEPLTAEDVLSIY
                     EKEKPEGVIVQFGGQTPLNLARELSEAGVRILGTTSETIDLAEDRDQFRHIIEKLGIP
                     QPDSGMASNLQEARTIAERIGYPLMVRPSYVLGGRAMKIILDEQMLREYVTKAVEVSP
                     DRPLLIDKFLEDAVEAEADAISDGTDAFVPAVMQHIEYAGIHSGDSACVIPSVTISKE
                     HKKTIYDYTRRIAMELNVVGLMNIQYAIYGDDVYILEANPRASRTVPLVSKVCNVSMA
                     AVATHILLGKKLPEFSLRNRPVKYYGVKEAVFPFNMFPAIDPLLGPEMRSTGEVLGIA
                     DTFGMAFFKSQEATQLPLPVKGTVLITVADRDKERILEIAGNFIKMGFTILSTSGTRK
                     FLEDHGIPAGLVLKVHEGRPNIVDAIKNGEIDLLINTPAGRLSEYDDSYIRTNAIRYK
                     LPYITTTSAALSATEGIRERHNGSYIVRSIQEYHASIGE"
     gene            complement(1939..2295)
                     /locus_tag="PLZ75_04325"
     CDS             complement(1939..2295)
                     /locus_tag="PLZ75_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71657.1"
                     /translation="MILLLMPPAMSVAGAVVLTVVLLLVAAAIGSVTTWFYAKSVYTP
                     VIKKLEEEKSGLQMQVADLNDEIRNLEKRIDSLKDDVSRLEKQVESLEENAAGLEKTL
                     EQKESQIKELKKKVKE"
     gene            complement(2297..2809)
                     /locus_tag="PLZ75_04330"
     CDS             complement(2297..2809)
                     /locus_tag="PLZ75_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOS71658.1"
                     /translation="MRIIIAGLLAFVIWCVFSAWMFNDNLLPVLKKQAVEPVPADNEA
                     RVADSLARLKASMPESLLVYFDFGKSEIKTDLQLESRIAPFRAWLRDHPDSKLLITGH
                     TDLIGTHDYNMQLGMERAQAVAGKVLELGIERNRIITESKGETQPAAGYISEENRSKN
                     RRTEITIKLP"
     gene            3300..3920
                     /locus_tag="PLZ75_04335"
     CDS             3300..3920
                     /locus_tag="PLZ75_04335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS71659.1"
                     /translation="MPLSSQSLKEDPALDEKVRKFLSENSRRWYDMNVPEVDGRLLYD
                     IIIKNGYRSALEIGTSTGHSGIWIAWALSKTGGRLITIDIDEGRHRRALENFRKAGLD
                     RYIDARLADAHVLVKELKGSFDFVFSDADKEWYKNYFIDIDPKLVVGGCFTAHNVYDR
                     RGRYGGYGAYGGPAEFLQYVRSLDNYETTVNSDGGGVSISYKKSEK"
     gene            3933..5297
                     /locus_tag="PLZ75_04340"
     CDS             3933..5297
                     /locus_tag="PLZ75_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOS71660.1"
                     /translation="MTEDKLQRKLGLFPATNIVIANMIGAGIFTTSGLLMSGLHNPLL
                     MLALWAIGGIIALCGAFSYGELGAAMPGAGGEYLFLSRLYHPIFGFLSGWVSFMVGFS
                     APIAASALGFSEYFSRAVPGFFIWIQDAGIMGPELTKKMLAVSIIVIFTFIHYRGIKY
                     GAGIQNVLTLLKVLLIVVLLIAGFGSGRGDFRNFSEGGKMLSGLSGFKTIGLSLMWIM
                     FSYSGWNASTYLGAEIKNPVRTLPRSLILGTGIVILLYLGVNVLYVYSIKPSEMKDVI
                     SVGGLAMGNLFGKPAEILVSLLIAFALFSSLSAFIIIGPRVYYSMAKDGLFFRSAARI
                     HERFKVPSNSILFQCLIAVILAFSGTFEQVLTYMGFALGIFPIMTVIGIWKLRKENPG
                     APRLKGFPYTQIIYIASGLLILILSFMEKPVESSVALLTVAVGIPAFFIFRRNNIKRN
                     GIIE"
     gene            5343..5525
                     /locus_tag="PLZ75_04345"
     CDS             5343..5525
                     /locus_tag="PLZ75_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71661.1"
                     /translation="MTDQEGRKEGRKEGRKERWKDGKKKERKKERKARRGERQVIPAR
                     SRMTTEAQAMPNAAGI"
     gene            complement(5456..6178)
                     /locus_tag="PLZ75_04350"
     CDS             complement(5456..6178)
                     /locus_tag="PLZ75_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOS71662.1"
                     /translation="MLSQWLERTEMLIGTESLKKLRESRVLVVGLGGVGAWAAEMICR
                     AGVGSMTVADGDRVQPSNINRQLLALESSVGMSKALLMGERLLGINPALNLTVIDEYV
                     RDEGIITVLDAGYDYVVDAIDSLSPKVFLLYHALARNCRIVSSMGSGGKFDPLKIRIT
                     DFSETSACTLARLLRKRLHKLGVREGFKAVWSPEATDKKKIIPVAGERNKASIVGTIS
                     YMPAAFGIACASVVIRDLAGIT"
     gene            complement(6265..6942)
                     /locus_tag="PLZ75_04355"
     CDS             complement(6265..6942)
                     /locus_tag="PLZ75_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOS71663.1"
                     /translation="MSLPDPGDYIDIHTHHGTHPEKGIFSVVSLMAHEEAGLLPLPGI
                     AYTFGIHPWHLSASSYEDHISGVRKAAADSRVLAIGEAGFDRLRGPDPELQRKAFEEQ
                     ALIAAEHHKPVIVHCVRAWDELLRARKRLRPARPWLVHGFRGSRDLAAQLISKDMFLS
                     FWFGFIIRPEAGKLIRSLPRERIFLETDGSGAGIRNIYVKVSADLDISVSGLKRIIYD
                     NFMDFFG"
     gene            complement(6939..8453)
                     /gene="gltX"
                     /locus_tag="PLZ75_04360"
     CDS             complement(6939..8453)
                     /gene="gltX"
                     /locus_tag="PLZ75_04360"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOS71664.1"
                     /translation="MQVRVRFAPSPTGPLHIGGIRTALYNYLFARKHNGTFILRIEDT
                     DQARLVPGAEEYIFESLDWCGIQVDEGIREGGDSGPYRQSERKELYGSYADMLISAGD
                     AYYAFDTPAELEQLRADAEKKGETFIYNASVRDRLQNSLALPEKMWKERMERGEPFVV
                     RYRMPSGEDIHFDDMIRGHIVVNSGTLDDKVLFKSDGMPTYHLANIVDDHLMEITHVI
                     RGEEWLPSLPLHVLLYRSFGWKAPEFAHLPLLLKPDGKGKLSKRDGDRMGFPVFPIFW
                     PYGETAKGYREEGYYPEAFVNMLALLGWNPGTEQEIFSMTELIDAFSMERVNRAGSRF
                     DPDKARWFNHQYLQHRSNRELALEYREILRTKGYHLDIGDIEILVGMVKERVNFVRDL
                     WQETDFFFIRPESYDADVIRKRWTAETPGQLLELRELLDGMDELRPDIAEQTVKSWIS
                     EKGYKTGAVMNAFRLVIVGASRGPGIFDIISWIGKQETLERIDRALELIGKDAL"
     gene            8619..9653
                     /gene="tdh"
                     /locus_tag="PLZ75_04365"
     CDS             8619..9653
                     /gene="tdh"
                     /locus_tag="PLZ75_04365"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOS71665.1"
                     /translation="MNTMKALVKARPEKGLWMQEVPIPEPGLNDVVIKVKKSAICGTD
                     LHIYKWDEWAAKTIKTPMIIGHEYMGYIEKMGAGVTNLKIGDRVTGEGHLACGHCRNC
                     RRGKEHVCENSIGIGVHIDGSFAEYVRIPAANVVPLDNRIPDDWAAIMDPFGNATHTA
                     LSFPLLGEDVLVTGAGLIGSMAVAIARYAGARFIVVSDLSDFRLDIARKMGASLTINP
                     SKGEKISDAVRQLGMRGFDVGLEMSGSPQAFIEMISNMYNGSNISLLGLLPSNFEIPW
                     DEIIFKAITLKGIYGREMWETWYKMEQMIIGGINLDPVITHRLHIDDFQEGFRIMEEG
                     KCGKVILNWY"
     gene            9733..10923
                     /gene="kbl"
                     /locus_tag="PLZ75_04370"
     CDS             9733..10923
                     /gene="kbl"
                     /locus_tag="PLZ75_04370"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOS71666.1"
                     /translation="MYTDYQKHLRKIIEEIKEAGLYKSERIIISPQGAEIELNTGQKV
                     LNFCANNYLGLSNNPGLIEAAKKALEDHGYGMSSVRFICGTSDLHKELERKISRFFMT
                     EDTILYASCFDANAGVFEPLLTEEDAIISDALNHASIIDGVRLCRAKRYIYPNADTDE
                     LEKQLKLAQSQRFRLIVTDGVFSMDGNVAPFRRIAELADQYRAMIMVDESHSAGVVGE
                     TGRGVTEFFGMPGRAEIITGTLGKAFGGAIGGFTTGKKEIIELLRQRSRPYLFSNSIP
                     PLVAAAGIEMIDMMTRNNELQDKLHRNSEYFISRISNLGFDIKPTRSAICAIMLYDAR
                     LSQEFAAKLLKEGIYVIGFSYPVVPKGEARIRVQLSAAHEREHLDTAVRAFEKIGRET
                     GVLK"
     gene            complement(10981..>13171)
                     /locus_tag="PLZ75_04375"
     CDS             complement(10981..>13171)
                     /locus_tag="PLZ75_04375"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOS71667.1"
                     /translation="VIGNEGSSDSYGGIMKIDQEQVQLMKRRGGVGHDLSHIRPRGTP
                     VLNSALTSTGVVPFMERYSNSTREVAQDGRRGALMLSISIKHPDSGKFIDAKMESGKV
                     TGANVSVKITDEFMKAVEQKSVFRQQYPIASANPKFTSDVDAVTLWNKIVHNAWKSAE
                     PGVLFWDTIIRESVPDCYADLGYATASTNPCGEIPLCPYDSCRLLAINLYSYVKNPFT
                     KEAEFDFELYKEHVGLAQRIMDDIIDLELEKIDAILEKVAADPEPDELKRVERNLWRN
                     IRRKTEEGRRTGIGITAEGDMLAALGLRYGTDKATDFSVEIHKTLALEAYKSSTYLAR
                     ERGPFSIYNSEREKNNPFILRLKEADPVLYNNMVKFGRRNIALLTIAPTGTTSLMTQT
                     TSGIEPVFSVFYKRRRKVNPNDKDARITFRDEVGDSWEEFNVFHHKFIEWLKVSGYDP
                     EKVMRMKDAEIESIIAKSPYYRATANDVDWIAKVRMQGAIQKWVDHSISVTINLPSDV
                     KEELVSELYLTAWKSGCKGATVYRDGSRAGVLVNGKPEARTERPKRPKVLNCDVIRFN
                     INEEKWVAFVGLKDNKPYEIFTGIADEEIFPIPKSIVKGQIIKHKPESGKSRYDFQYT
                     DKYGYKKTMEGLSHMFKPEFWNYAKLISGVLRHGMPIADVVNLVQSLKLDSESINNWK
                     NGVERALKKYIPNGTRAKGKCSECHSENLVYEEGCLICKDCGSSKCG"
BASE COUNT         3415 a         3177 c         3125 g         3454 t
ORIGIN      
        1 tgaaggaact ggtggatgag gaagaggcta tactcggata caggggatca atgcctcccg
       61 atgacgtcct ggcaagcgcg aaaagaaatg gttttgccga caaatacctg tcacagctgc
      121 tggggattcc cgagaaggat atcagaacaa agcgtgtttc cctgggtatc agggaagcat
      181 gggagcccgt acctgtcagc ggggttgaag atgaagcata ttatttttcc acatacaatg
      241 gtcccgacaa ggttgggata actgacagaa agaaaataat ggttctggga ggcggtccga
      301 acaggatagg ccaggggatt gaatttgact actgctgtgt acatgctgcc tttgcaatcc
      361 gggatgccgg ctatgaatcg ataatggtaa actgcaatcc cgaaaccgtt tccactgact
      421 atgacacagc cgacagactg tattttgaac ctctgacggc cgaggatgtt ctgagcatct
      481 atgaaaagga aaagcccgag ggagtcattg tacagttcgg aggccagacc ccccttaatc
      541 ttgcacgtga actgagtgaa gccggggtga ggatactcgg aaccacttcc gaaaccattg
      601 acctggcgga agaccgcgac cagttcaggc atataatcga aaaactggga atcccccagc
      661 ctgattcagg tatggcaagc aatctccagg aagcccggac aattgctgaa aggattggtt
      721 atccgcttat ggtaagacct tcctatgtgc tcgggggaag agcaatgaaa atcattctgg
      781 atgaacagat gctgagggaa tatgttacaa aagccgtgga agtgtctccc gaccgacctc
      841 tgctcattga caagttcctt gaggatgccg ttgaggccga ggcagatgca atatctgacg
      901 ggactgatgc atttgtgccg gcggtaatgc agcatatcga gtatgcaggc attcattccg
      961 gcgattctgc ctgtgtgata ccttcggtta caatatcaaa ggagcacaag aagacgatct
     1021 atgactatac ccgcaggatt gcgatggagc tgaacgttgt gggactgatg aatatccagt
     1081 atgcaatcta cggggatgac gtatatattc tggaagcaaa tcccagggcc tcgagaacgg
     1141 tgcctctcgt ttcaaaggtc tgcaacgtgt caatggcagc tgttgcaaca catatcctgc
     1201 tcggtaagaa actgcctgaa ttcagtctca ggaacaggcc ggtaaagtat tacggcgtga
     1261 aggaagctgt ttttccattc aacatgtttc ctgccataga cccgctgctg gggcccgaga
     1321 tgcgctccac cggagaggtg ctgggcattg ccgatacctt cggaatggca ttcttcaagt
     1381 cgcaggaagc aacacaactg ccgcttccgg tgaaggggac ggttcttatc accgtggcag
     1441 accgcgacaa ggagcgcata ctggaaatag ccggtaactt tatcaaaatg ggattcacca
     1501 tcctgtcaac ttcaggaacc agaaaattcc ttgaggatca tggaatacct gccggactgg
     1561 tactgaaagt ccatgaagga aggccgaata ttgttgatgc aataaagaac ggtgaaattg
     1621 atcttcttat aaacactccg gccggacgac tgagtgaata tgacgattca tacatcagga
     1681 caaacgccat cagatacaag ctgccctata taaccactac ttctgctgcc ctttcagcaa
     1741 cggaaggaat aagggaaagg cataacggat catatattgt gagatccatt caggagtacc
     1801 atgcttcgat aggagaatag tttccgtccg gttaagggca acgggctgcc ggccggcggc
     1861 cccgggcatg ccgacactgc ggattgatat cgggaatacc ctgtatgcac tttaagccac
     1921 accagtcttt cagctgtgtt attccttcac ctttttcttc agttccttta tctggctttc
     1981 cttttgttcc agagtctttt caagtccggc agcattctcc tcaagcgatt caacctgctt
     2041 ttcaagcctg ctgacatcgt ccttcaggga atcaattctt ttttccaggt ttctgatttc
     2101 atcattcagg tcggcaacct gcatctgaag gccggatttt tcctcttcaa gctttttaat
     2161 aacgggggta tacactgatt ttgcatagaa ccacgtcgta acagaaccta ttgctgccgc
     2221 aaccaggagc agtacaaccg tcaggaccac agctccggcc acggacattg cgggaggcat
     2281 taataataga atcatatcac gggagtttaa tagttatttc tgttcttctg ttttttgacc
     2341 tgttttcttc gctgatatat ccggctgcag gctgggtttc accttttgat tcagttatta
     2401 tcctgttcct ttcaattccc agttccagga ccttgcctgc aacagcctga gctctttcca
     2461 taccgagctg catattgtag tcatgtgtgc cgatcaggtc agtgtggccg gttataagaa
     2521 gctttgaatc aggatgatcc cggagccatg ctctgaatgg agcgatcctg ctttccagtt
     2581 gcagatctgt tttgatttct gatttgccga aatcaaaata aaccagtagt gattcaggca
     2641 ttgatgcttt aagccgggct aaagaatctg ccactcttgc ctcattgtcg gcaggcactg
     2701 gctctacagc ctgtttcttc agtaccggaa gcaaattatc attgaacatc catgccgaaa
     2761 aaacacacca tatcacaaaa gccagtaaac ctgcaattat tattcgcatt gctgaaatga
     2821 tttaagttac tgatcaggta attttcctac aagttaccaa aaaaatctaa aataaaggat
     2881 taaaaacctg caataaaatt gacctgacta cagcagcact gcaaatctcc cggactcatc
     2941 ccgccgaaaa caagccgtga ttcctgtctg acggttttta taactgtttc ccggcaaacc
     3001 gggcacaagg ttaaaagtta ttaaaaaaat ataattaaaa gccttcacgt gtcccgtgcg
     3061 gaataataaa gtatatttat ctgtctatag ttttaaaaag acttattcca tcattcaata
     3121 taaaaatgaa acccgaatag ttgatcacgt tatcaaatac aaaaaaatat aatttgcagt
     3181 catgcatggt ttcccgcttt ttgcaggaca ggctgtaata ccattaaaat cagaattaca
     3241 ccatgacgaa aggtattttg ttttttcctt tatgtatctc acttataatt ttcaccggta
     3301 tgccgctgag ttcccagtcc ttaaaggaag accccgccct tgatgaaaaa gtgaggaaat
     3361 tcctgtctga gaattcccgc cgctggtacg acatgaatgt tcctgaagtg gatggcaggc
     3421 ttctgtatga cataattata aagaacggat acaggtccgc tctggaaata ggaacttcca
     3481 cgggtcattc aggcatctgg atagcctggg cattaagcaa aacggggggc aggctgataa
     3541 caattgatat tgatgagggc aggcaccgca gggctcttga gaatttcagg aaagcaggac
     3601 tggacagata cattgatgcc cggctggccg atgcccatgt tcttgtaaaa gagctgaaag
     3661 gatctttcga tttcgttttc agcgatgcag acaaggaatg gtataagaat tatttcattg
     3721 atattgatcc caaactggtt gtcggtggtt gttttacagc tcataacgtt tacgacagaa
     3781 gaggaagata cggcggatac ggagcctacg gaggtccggc tgaatttctg cagtacgtca
     3841 ggagccttga taattatgaa acaacagtca acagcgacgg cggaggtgtc tcaataagtt
     3901 ataagaaatc tgaaaaataa tcccggctga ttatgacgga agataaacta cagagaaagc
     3961 tgggactctt ccctgctaca aacattgtta tagccaacat gatcggcgca gggatcttca
     4021 cgacatcagg gcttctgatg tcgggccttc acaaccccct gctgatgcta gccctctggg
     4081 ccataggagg tattatcgca ctttgcggcg cattctccta tggtgagctc ggagcggcaa
     4141 tgcccggtgc gggaggggaa tatcttttcc tttcaagact ctatcatccc atctttggct
     4201 ttttaagcgg atgggtctcc ttcatggtag gattctccgc tcccattgcc gcatcagccc
     4261 ttggcttcag cgagtatttc tcaagagcgg tgccaggctt cttcatatgg atacaggatg
     4321 caggaataat gggaccggaa ctgacaaaga agatgcttgc cgtaagtata attgtaatat
     4381 tcaccttcat ccattacagg ggaataaaat acggtgccgg aattcagaat gtcctcaccc
     4441 tgctgaaagt ccttctgatc gtcgtattgc tgatagccgg attcggatcg ggaaggggag
     4501 atttccggaa tttcagcgaa ggcggaaaaa tgctgtcagg attgtcaggg tttaaaacaa
     4561 taggcctttc acttatgtgg atcatgtttt catacagcgg atggaatgct tcaacctatc
     4621 tgggtgctga aataaagaat cccgtccgga cgctgccccg ttcactgatt ctcgggaccg
     4681 gaatcgttat actgctgtat ctgggagtaa atgttctgta tgtttacagc atcaaaccct
     4741 ccgaaatgaa ggatgtcatc tcggtaggcg gtctggcaat gggcaatctg ttcgggaaac
     4801 cggcagaaat ccttgtttcg cttcttattg cctttgccct gttttcttcc ctcagtgctt
     4861 tcataataat cggccccaga gtgtattatt caatggcaaa ggacggcctc ttcttcagat
     4921 cggcagccag aatacatgaa agatttaaag tgccctccaa ttcaatcttg tttcaatgtc
     4981 tcatagcagt gatactggct ttctcgggca ctttcgagca ggttctgact tatatgggct
     5041 tcgccctcgg aatatttccc atcatgaccg tcatcggcat ctggaagctc aggaaggaaa
     5101 atcccggagc tcccaggctc aaaggatttc cctatacgca gattatatat atagcttcag
     5161 gattgctcat acttatactc tcgttcatgg aaaaaccggt tgaatcctcg gttgccctgc
     5221 tgactgtagc cgtggggatt ccggcatttt tcatcttcag gcggaacaat ataaagagaa
     5281 acggaatcat cgagtaaggt tcgctgatga tcaggaataa taatttagaa agccgtgcgg
     5341 gtatgactga tcaggaagga aggaaggaag gaaggaagga aggaaggaag gaaagatgga
     5401 aagatggaaa gaagaaagaa agaaagaaag aaagaaaggc aagacggggt gaacgtcagg
     5461 ttatcccggc aaggtcgcgg atgacaacgg aagcacaggc aatgccgaat gcagcaggca
     5521 tataggaaat ggttccgact attgaagcct tgtttcgttc ccctgcaacc ggtatgatct
     5581 tctttttatc cgttgcctcg ggcgaccaga ctgccttgaa cccctccctg acacccagct
     5641 tgtgaagcct tttcctgagg agccgggcca gcgtacaggc gctggtttcc gaaaagtcag
     5701 tgattcttat tttcagcgga tcaaatttcc cgcctgatcc cattgaactg acaatccggc
     5761 aattccgggc gagggcatgg tacaggagaa agaccttggg ggaaagagaa tctatggcat
     5821 ccactacata gtcgtaaccc gcatcaagaa cagttattat tccttcatcc ctcacatatt
     5881 catcaataac agtaagattg agtgcggggt ttatccccag cagtctttcg cccataagaa
     5941 gggcttttga catgccgaca ctgctttcaa gcgccaggag ctgcctgttg atgtttgaag
     6001 gctgaaccct gtccccgtcc gcaacagtca tcgagcctac tccggccctg catatcattt
     6061 ccgcagccca ggcccccact ccgcccaatc cgacaacaag aacccgggat tcccggagct
     6121 tcttcaggct ctctgtcccg atcagcattt cagtccgttc aagccactgt gacaacatga
     6181 tgatcaattt ttaacaacaa cggcatttca cgcctggtgt aaaaataagg aacaggctgc
     6241 ataaaccggt ccggagaaat catttcatcc gaaaaaatcc atgaaattat cgtagataat
     6301 acgtttcagt ccggaaacag aaatatccag gtcggcagaa acctttacgt aaatattcct
     6361 tatcccggct cctgatccgt ccgtttcaag aaatatcctt tcccggggaa ggcttcgtat
     6421 cagttttccg gcttcgggcc tgataatgaa accgaaccag aatgaaagga acatgtcttt
     6481 tgatatcagc tgggcggcaa gatccctgct tcccctgaaa ccgtgaacaa gccagggtct
     6541 tgcaggcctg agtcgttttc gggcccggag cagttcatcc catgccctga cacaatgaac
     6601 aatcacaggt ttatggtgtt ccgcggcaat cagagcctgt tcttcgaatg cctttctctg
     6661 aagctccgga tcaggtcccc tcagcctgtc aaagcctgct tcgcctattg caagcacccg
     6721 gctgtcagcc gcggccttcc ttactccgga aatatgatcc tcataggatg aagcactgag
     6781 atgccaggga tgaatcccga aggtataggc gatcccggga aggggaagaa gtccggcttc
     6841 ttcatgtgcc atcaggctta ccacggaaaa gatgcccttt tccggatggg tgccgtgatg
     6901 ggtgtggata tcaatataat caccaggatc cggcaggctc ataatgcatc ttttcctata
     6961 agctccagtg ccctgtcaat tctttcaagg gtttcctgct ttccgatcca ggaaataata
     7021 tcaaaaattc ccggtccgcg tgaggcgccg acaataacaa gcctgaaggc attcatcacc
     7081 gcaccggttt tataaccttt ctctgaaatc catgacttca ccgtctgttc tgcaatatca
     7141 ggcctgagtt catccatgcc gtccagcagc tccctcagtt ccagcagctg tccgggagtt
     7201 tcggcagtcc atctctttct gatgacatca gcatcgtagc tctccggcct gataaaaaag
     7261 aaatccgttt cctgccatag atcccgcacg aagttcaccc tttccttcac cattccaacg
     7321 agtatttcaa tatcaccaat gtcgagatga tagccttttg ttctgagtat ttccctgtat
     7381 tccagagcca gttccctgtt gcttctgtgc tggagatact ggtggttgaa ccatctggcc
     7441 ttgtcagggt cgaacctgga tcccgccctg ttgactcttt ccattgaaaa ggcatctatc
     7501 agttctgtca tgctgaatat ttcctgttcc gtaccgggat tccatccgag cagtgccagc
     7561 atgttgacaa aagcttccgg ataatatcct tcttccctgt atccttttgc cgtttcccca
     7621 tagggccaga atatcggaaa cacggggaat cccatcctgt caccgtcgcg cttgctgagc
     7681 ttccctttgc cgtcgggctt gagaagcagt ggcaggtgtg cgaattcagg tgccttccat
     7741 ccgaaagaac ggtaaagcaa aacatgaagg ggaagtgaag gcagccattc ttccccgcgg
     7801 atgacatgag tgatctccat cagatgatca tcaacaatat ttgcaagatg ataggtgggc
     7861 atcccgtcgg atttgaaaag caccttgtcg tcaagggttc cggaattcac gacaatatgc
     7921 ccgcggatca tgtcgtcaaa atgtatgtct tcgccggagg gcatcctgta cctgacaaca
     7981 aagggctctc ccctctccat cctctctttc cacatctttt caggcagtgc gagggaattc
     8041 tgcagtctgt cccgtacaga tgcattgtaa atgaaggtct cccctttttt ctccgcgtcc
     8101 gccctcagtt gttccagctc agcgggagta tcaaaagcat aataggcatc accagcagaa
     8161 ataagcatat ccgcatagct gccgtacagc tcctttcttt cgctctgcct gtagggcccg
     8221 gaatctcctc cttcgcgaat accttcatca acttgtattc cgcaccagtc cagtgattca
     8281 aaaatgtatt cctcggctcc cggaacaagc ctcgcctggt ctgtgtcttc tattctgaga
     8341 ataaaagtac cattatgctt tcttgcaaaa agataattat aaagagccgt ccgtataccc
     8401 cctatatgga gagggcccgt cggactgggc gcaaatctta ctcgtacctg cattgagaaa
     8461 taatttgatt aatcctgtaa agataactaa agccttttac tttaaactgc atgcagtaat
     8521 catcattatt tccgtatttt tatctgcctg tgggtttata ggaatttttc atgtacattt
     8581 gagtactcct gttatgtttc atctcaaatt tcaaaggcat gaacacgatg aaagcactgg
     8641 tcaaggctcg tcccgaaaag ggtctctgga tgcaggaagt accgattccc gaaccgggtc
     8701 tgaacgatgt tgtcataaag gtcaaaaagt ccgccatctg cggcaccgat ctccatatct
     8761 acaaatggga tgagtgggcg gcgaaaacca ttaaaacccc gatgatcatc ggccatgaat
     8821 acatgggtta cattgaaaaa atgggagccg gtgtcacgaa tctcaaaatt ggcgacaggg
     8881 ttaccgggga aggtcacctt gcctgcggac attgcaggaa ctgccgccgt ggaaaggaac
     8941 atgtatgtga gaattccata ggtatcgggg ttcatatcga cggatcattt gccgaatatg
     9001 taaggattcc ggctgccaac gtggttcccc ttgacaaccg cataccggat gactgggcag
     9061 caattatgga tcctttcgga aatgcaaccc atacggccct ttcattcccg cttctcggag
     9121 aggatgtgct ggtaaccggt gccgggctga taggaagcat ggccgtggct attgcacgat
     9181 atgcgggagc gaggttcatc gtagtgagtg atctgagcga tttccggctc gatatagcaa
     9241 ggaaaatggg agcaagcctt accataaatc cttcaaaggg agaaaaaata tccgatgccg
     9301 tcaggcagct cgggatgaga ggatttgatg tgggcctgga aatgtcgggg tctcctcagg
     9361 cattcataga aatgatttcc aacatgtata acgggtcgaa tatttccctg cttggccttc
     9421 tgccttcaaa cttcgagata ccctgggatg aaataatttt caaggcaata accctgaaag
     9481 gaatctatgg cagggaaatg tgggagacct ggtataagat ggaacagatg atcataggtg
     9541 gcataaacct tgatcccgtg ataacccacc gcctccatat tgatgatttc caggagggat
     9601 tcaggataat ggaagagggt aagtgcggaa aggtcattct caactggtac tgaagggaaa
     9661 ttaccggaaa acccatcccg gcgtatgaat tccgcgtaac aagattaaaa caccaagact
     9721 aaaacacagg atatgtatac agattatcag aagcacctta ggaaaatcat tgaagaaata
     9781 aaggaagccg gtctctacaa gagtgaacgg ataatcatat ctccccaggg tgctgaaata
     9841 gagcttaaca cgggccagaa ggtcctgaat ttctgtgcca acaactacct cgggctgtca
     9901 aacaatcccg ggctcatcga agctgcaaaa aaggcccttg aggatcacgg atacggaatg
     9961 tcgtcagtaa ggtttatctg cgggacaagc gaccttcaca aagaactgga gaggaagatt
    10021 tccaggtttt tcatgacgga agatactata ctctatgcat cctgtttcga tgcaaacgcc
    10081 ggggtttttg agcctcttct cactgaggag gatgccataa tctcggatgc tctgaaccat
    10141 gcatccataa ttgacggggt ccgcctgtgc agagcaaaaa ggtatattta tccgaatgcc
    10201 gacacggatg aactggagaa acagctcaag ctggcgcagt cacaaaggtt caggctgata
    10261 gtgactgacg gggttttttc catggacggc aatgtggcac ctttcagaag aatagccgag
    10321 cttgcggacc agtacagggc catgataatg gttgatgaat cccattccgc cggtgttgtt
    10381 ggcgaaaccg gaaggggggt taccgaattc ttcggcatgc cgggccgcgc agagattatc
    10441 acgggaactc tcggaaaggc tttcggaggg gcaataggcg gtttcacaac gggaaaaaag
    10501 gagataattg aacttttgcg ccagagatcc aggccctacc tcttctccaa ttcaattccg
    10561 ccccttgtgg ctgctgcagg gattgaaatg attgacatga tgaccaggaa caatgaactg
    10621 caggataagc tgcaccgcaa ttcggagtat ttcattagca gaatatccaa tctcggattt
    10681 gatatcaagc ccaccaggtc ggcaatctgc gcaatcatgc tctatgatgc aagactgtca
    10741 caggaatttg ccgcaaaact gctgaaggaa gggatatatg ttatcggatt ttcctatcct
    10801 gttgttccga agggtgaggc aaggatcagg gtgcaactgt ctgccgccca tgaaagagaa
    10861 catctggata ctgctgtaag ggcttttgaa aagataggca gggaaacggg agtattgaag
    10921 taagtctccc cgggtgtcaa tggccgcatt taattaccgg caatttttgc ctgcaacctt
    10981 ttatccgcac tttgatgatc cgcaatcctt gcagatgagg cagccttcct cataaacaag
    11041 gttctcggaa tggcattcac tgcatttccc cttggctctg gtcccgttgg gaatatattt
    11101 cttcagggcc ctctcaactc cgtttttcca gttattgatg gattcactgt caagtttcag
    11161 tgactggacc aggtttacca catcggcaat cggcattccg tgcctgagca cgccggaaat
    11221 aagcttggca taattccaga actccggttt gaacatatgg gataagcctt ccattgtttt
    11281 cttgtagccg tacttgtcgg tatactggaa atcgtaacgg cttttgccgc tttccggctt
    11341 gtgctttatt atctgtccct ttacaatgct cttgggaatg ggaaatattt cctcatctgc
    11401 aatacccgtg aagatctcat agggcttgtt gtccttcagc ccgacaaagg ccacccattt
    11461 ctcttcattg atattgaatc ttattacatc acaattaagt accttcggcc gtttcggcct
    11521 ttccgtcctg gcctcgggtt tgccgtttac cagtacccct gcccttgatc cgtcgcgata
    11581 cacggtagca cccttgcaac ccgatttcca ggctgtgaga tataattcgc tcacgagttc
    11641 ctccttcacg tccgaaggaa ggtttatggt aacacttatg gaatggtcaa cccatttctg
    11701 tattgctccc tgcattctca cctttgcaat ccagtcgaca tcattggcgg ttgccctgta
    11761 ataaggtgac ttagcaatga ttgattctat ctccgcatct ttcatccgca tcactttctc
    11821 cggatcataa ccgctgacct taagccattc gataaattta tgatgaaaga cattgaattc
    11881 ctcccacgaa tctcccactt catccctgaa agtgatcctg gcatccttgt cattgggatt
    11941 cacttttctc ctgcgcttgt aaaacactga aaacaccggt tcaatgcctg aagttgtctg
    12001 ggtcatcagg ctggtggtcc cggtaggggc gatggtaagc agggcaatat tccttcttcc
    12061 gaatttcacc atattgttgt aaagcacagg atctgcttcc ttcagtctca gaataaacgg
    12121 attgtttttt tccctttctg aattgtaaat actgaagggt cctctttccc tggccagata
    12181 ggttgaagat ttataggcct cgagggcaag ggttttatga atttcaacgg aaaaatccgt
    12241 cgccttgtca gtaccatacc tcaatcccag ggcagcaagc atatcccctt cggcagttat
    12301 tccgatgcct gttcttcttc cttcctcggt tttcctgcgg atattcctcc agagattgcg
    12361 ttccaccctt ttcagttcat cgggttcagg atcggcagcc accttttcca gtatggcatc
    12421 tatcttttcc agctccaggt cgattatatc atccattatc ctctgggcca ggcccacatg
    12481 ctccttgtag agctcaaaat caaattcagc ttcctttgta aaggggtttt tcacatagct
    12541 gtacaggttg attgcgagaa ggcggcagct gtcataggga cagagaggta tttccccgca
    12601 ggggtttgtg gatgccgtcg catagcccag gtcggcatag cagtcaggca cactctccct
    12661 tatgatggta tcccagaaaa gtacgcccgg ttcggcggat ttccatgcat tatgcacaat
    12721 cttgttccag agtgttactg catccacgtc gctggtgaac ttcggattgg cagaagcgat
    12781 cggatactgc tggcggaata cgcttttttg ctcaacagcc ttcatgaact catcggtaat
    12841 cttaaccgat acattggctc cggtaacctt gccgctttcc atttttgcat cgatgaactt
    12901 gccggaatca ggatgcttga tggatattga aagcatgagg gcacctctcc ttccgtcctg
    12961 tgccacttcg cgtgtggagt ttgaataacg ttccatgaaa gggacaacgc ctgttgaagt
    13021 gagggcactg ttcagaaccg gtgttcctct cgggcgtata tgggaaagat catgacccac
    13081 ccctcctctt cttttcatca gctgtacctg ctcctggtca atcttcatta tgccgccgta
    13141 ggaatcggag gacccttcat tgccgataac a
//
LOCUS       DAOSPX010000055        13085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4795_length_13085_cov_3.990637, whole genome shotgun sequence.
ACCESSION   DAOSPX010000055 DAOSPX010000000
VERSION     DAOSPX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4795_length_13085_cov_3.990637"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..3678
                     /locus_tag="PLZ75_04380"
     CDS             109..3678
                     /locus_tag="PLZ75_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71668.1"
                     /translation="MPARITVYKDDTLRITVKTPEFIFTKAGLTGPETSMAIYIGKDS
                     IYHSNLSFSYNAEQHQVNFFRGNNPISRSPSFNSFHMLDMYFEYLSWNLDDSKIVFSR
                     SRGSSIGKAHFESASFFDSRLFMDLAGIDEYHPLMQLKKFSEWYYSETFPVAEFAAWL
                     NRPAEAVTGLCIDLANRGFLFYDRTYNEVTLKKKVNDFLTAFAKKKDYDILRFNSETK
                     APLDNAVLDTKNNQMTVNGVSNVHLSDSQKVAIFPYNQQIRIGRNRDMDFDGVVEAGL
                     FTIFGHNFRFSYDTFKINLANIDSIRIAVEIDEVDAYGNPLVKAMDNLLELTNADLFI
                     DDPDNKSGLKNLTQYPMVGVKKDSYIFYDNIPGLENVYPREDFYFRIFPFVYENIDHF
                     KIETINLPGEFAGGNILKPMRQNLIVKDSSLLGFDMIIPPEGIELYGGKAVLYDSISM
                     GSKGLRGKGTIKHLASTIVSEQFYLYPDSTITRATSFSMGGDVSGRFPSLESHNVAMR
                     WLTGENEMQAVSSRGNSFRMFDNSSDFDGNIAYTPKSMTGTGIFNTSDSRITSDGFSF
                     TSTSVKTDTSDFFLKSPSTGGYAFIAENAAVNVDFTLKTTALRLNTDDSFIKFPEIQY
                     ISKLSDFEYDMQDRILRMQEKRKAEKELVTPDRLLRTDLKNPEKPNFFATSTIRDTIS
                     FNAQKASYNLDGEYIEAENINYIRIADALIQPEKGRIKIGRRAMIDPLQNAVVAINNR
                     HLLHSAGINIESARKYSGRGIYDYTDDSGEVRNISFSEVTVDTMATSARGFITSGENF
                     MLSPAFTFTGDVNLYSGQEFLLFTGAAGIVNTCPSVKSAPVQFKSFIDPRNVMIPASD
                     KPRDINGNPVMTGSLLNIDAMTIYPAFLSEQRSWIDVGLVSSRGYLYYDKAKSSYLLT
                     SLEKIADRTLHGDLIALDRNSCILSGEGRINLGARFDPVKMTGAGQIKHNIDTGYVEI
                     QALLGFDFHFSPEALRIMSQELKFIPTLKPVNLNSELYTKGMRDLFGVETANRMKEDL
                     DLFGVSGELPPEFTFRLFLNDVTLYWHEASASFRSKGRIGLGFIGTDPVNVYVDGMIE
                     IQRRRAGDMFDIYLKANESTWYYFSYIKGNMMTLSSNSAYNSVIENTRQRLRRIPKKV
                     SREPYSYMIAVRDRMIRFLRRMESSDEEERSPAQDDPLRNLVR"
     gene            3719..4423
                     /locus_tag="PLZ75_04385"
     CDS             3719..4423
                     /locus_tag="PLZ75_04385"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOS71669.1"
                     /translation="MQIFYAPDISDDIYILDEKESRHSVRVLRLKKGDPAGIIDGKGN
                     LYQGTIIDPDVKHCSVRILNKIENYEKRNYSLHIAISPLRNPGRLEWFTEKCVEIGTD
                     EITPLICTNTEKHSIKKDRLQNLIISAMKQSLKAKLTKLNDPSGFNDFISMKRKGKLI
                     IAHCNSRFSRDSIKDVYGKGEDAVILIGPEGDFSEEEILAATERDYCPVHLGPSRLRT
                     ETAGIAACHSVYLINQ"
     gene            complement(4441..4626)
                     /locus_tag="PLZ75_04390"
     CDS             complement(4441..4626)
                     /locus_tag="PLZ75_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71670.1"
                     /translation="MSGKKAFVLRLDPGKLKAVEKWAADEFRSTNGQIEYLLDQALRK
                     AGRWKPADHSPPAGEKK"
     gene            complement(4629..5492)
                     /locus_tag="PLZ75_04395"
     CDS             complement(4629..5492)
                     /locus_tag="PLZ75_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOS71671.1"
                     /translation="MQEMKHEYSVKPVSGYVSLPFAAIFLAGAIVGFAFEIIWLGSIL
                     AILFVLAVFGYTIVYPNGSCVMVLFGAYKGTIRENGFFWVNPFYTKRKISLRARNFDS
                     EPIKVNDKRGNPIKIGFVLVWRVLDTYKAVFDVDDFKNFVLVQSDAALRKLAGLYPYD
                     NFDDNVEEIALRSGGEEINDELENEIRDRLKIAGVEVIEARINYIAYSEEIANAMLRR
                     QQAAAVVSARHKIVEGAVSMVQMALEQLSDKMIVDLDEERKAAMVSNLMVVLCSDKDT
                     TPVVNTGTLYN"
     gene            complement(5584..5769)
                     /locus_tag="PLZ75_04400"
     CDS             complement(5584..5769)
                     /locus_tag="PLZ75_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71672.1"
                     /translation="MDLISTQFVKYQEIKTRDIDLHEDAFGLYQQFDDVIGYLNMLKP
                     EPPKNLTRRLITRIRKH"
     gene            complement(5859..6446)
                     /locus_tag="PLZ75_04405"
     CDS             complement(5859..6446)
                     /locus_tag="PLZ75_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS71673.1"
                     /translation="MSKTISDYELIQRFVKGEQSCFNQIIHRHKNKVFAYISLYIRDQ
                     ALAEDIFQDTFMKVIQSVKKGRYQDNGKFVSWVMRIAHNLIIDHFRRAKQMNTISNNE
                     YESDLFNSRKLSDSTIEDNLIRRQIQKDVRKMISCLPDDQREVVILRHYAGLSFKEIA
                     DITNVSINTALGRMRYALINMRKIMEEKKISLTLN"
     gene            6603..9425
                     /gene="uvrA"
                     /locus_tag="PLZ75_04410"
     CDS             6603..9425
                     /gene="uvrA"
                     /locus_tag="PLZ75_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOS71674.1"
                     /translation="MNGCIEIRGARVHNLKNISLKIPRNKLVVITGVSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGRMNKPEVDFISGIPPAIAIEQKIKSRNPRSTVGTS
                     TEIYDYLRLLYGRIGRTVSPVSGKEVKKHSVDDIVEFLASLQEDSRVIITAAGQIPEN
                     INIGDYFSFLRKQGFDRIETGGKIIRISEINEDDFKNGQKINIVIDRLIIDHSPDNFS
                     RAADSAQTAFHTGQGYCEVLILNDDGPPDREGFSTRFEADGIVFEEPSEYLFSFNNPL
                     GACPLCEGYGRIIGIDEDLVIPDRNLSVYDDAIACWKGESMRRWKERLISSAHLFDFP
                     VHKPYFQLSDEQKALLWKGNRYFYGLDRFFRYLEEKKYKIQYRVMLSRYRGKTVCPEC
                     RGSRLRKEAGYVKIAGKTIQELADMPANRLYDHFSEIKLDSPGEEMVAGRLLTEINTR
                     LRFILDVGLSYLTLNRLSSTLSGGESQRINLSSSLGSNLVGSMYILDEPSIGLHPRDT
                     ALLVRVLRELRDLGNTVIVVEHEEEIIKAADHIIDLGPEAGRLGGEIVFQGSPAMAAS
                     GGSLTAGYLAGTMKIAVPSSRRKWNSFIEIVGARENNLKNIDVKFPLHTITAVTGVSG
                     SGKSSLVTEILHKYLSNRITGSNLQPGQFREIRGDITGLTGIELIDQNPIGKSSRSNP
                     VTYLKAYDEIRKLFAGQQASVQNNYKPSHFSFNIKGGRCEECQGEGIIRVEMQFMAPV
                     ELTCEECRGMRFKRDILEVKYHGKNIHDLLEMTIDEAIEFFSGHPGKTESKIVEKLKP
                     LSEVGLGYIKMGQSSSTLSGGESQRVKLAYFLSQEKASGHILFIFDEPTTGLHFHDIN
                     KLLKSINALVEHGHSVVVIEHNPEIIKSCDWIIDLGPEGGNEGGYVVFEGKPEDLVNC
                     AKSYTGKYLLHNLEKNSKAPLKTGHR"
     gene            complement(9446..9889)
                     /locus_tag="PLZ75_04415"
     CDS             complement(9446..9889)
                     /locus_tag="PLZ75_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71675.1"
                     /translation="MKTLKNILLPVILLLFSGGFAGAQCKAFAKKECLPNLAGYTHDG
                     NYHAAVLVEGEEAELYKTFYSDMNYRVSIVGDSKLPDIEFRVLDSNKNVLYTNKDKNY
                     SSTWDFKLESSQQLKFLIKVPSATRSGQQPSSGCVAIMFGFRLKK"
     gene            complement(9896..10816)
                     /locus_tag="PLZ75_04420"
     CDS             complement(9896..10816)
                     /locus_tag="PLZ75_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71676.1"
                     /translation="MTRQFRISSGIGIGIVFILGIILFTCCKGGRKAPEVNLELDIRD
                     KEAVIQDIKQAEKIFQALPSPLESAMLIKSAGARFEEKLLNPVSNVNKYVTNKSMALN
                     LGIYTCDLSFASLYEQTQLLIDYMNAAKKMADGLGILKAIEQKDIDRLEENINNSEVI
                     MDIVSQTFMNSNSYLEDNGQPATAAMVLTGGWIEGLYISTQLVDMNDFDGNKLVGRII
                     DQKLSVDILLRLLSGSKGTAAVDELMVEVEKLKNVFDKIRLDTSPVRPEYDPDSRSTV
                     LKSETRTDMTPAVFLELSAVVKEIRNSFVI"
     gene            10951..11469
                     /locus_tag="PLZ75_04425"
     CDS             10951..11469
                     /locus_tag="PLZ75_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71677.1"
                     /translation="MKKSEKRYRELEGILKKGRVSLISEAIRSLREEEPFEGAIELLA
                     EHYNVTDDKMNIIAIEEFFNDIKYLSARSEIMAEIRKPSYKQKTISMLVSSCWQSGLD
                     YSEYVQDIVRVFLEGDYTTAIECMTLIEESLRYNTREDKDKAIHLVERSPEAFTHEKD
                     PLSRELISILAR"
     gene            complement(11557..12534)
                     /locus_tag="PLZ75_04430"
     CDS             complement(11557..12534)
                     /locus_tag="PLZ75_04430"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOS71678.1"
                     /translation="MGKLSVKYLGLDLKSPLIVSSSRLTSSLDHLKEAEDNGAGAVVL
                     KSLFEEQIDHYIHTIPVSADYPEAGDYVSYYMRSNRVDEYLSLIKSAKESLSIPVIPS
                     INCYSSGGWTGFAGNIAEAGADAIEINVFFLPDDRKKSATDSEKVYLELVEKLKKTIK
                     IPIALKIGFRFSNILNIIDQFYMRGVEGIVMFNRFYEPDIDVDKMDIVPAPVFSREEE
                     MRYVLRWIGMASAQDIKIDISASTGVHSGHHAIKYILAGANSVQVCSVLYNKGLQFLK
                     VMNTQIEEWMERKEFRSVDEFRGRLNYRNYGRPVVYERTQFMKYFAAED"
     gene            complement(12555..>13085)
                     /locus_tag="PLZ75_04435"
     CDS             complement(12555..>13085)
                     /locus_tag="PLZ75_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOS71679.1"
                     /translation="ENRVQELLSKKDLLPGDIEWHLIGHLQTNKVKYVVPFVSMIQSV
                     DSFKLLRAVNTAALKINRRIDCLLQFHIAEEETKYGFALKEVRELAGSDEVRRMEAVR
                     ICGVMGMATFTSDMEQVRDEFRYLAACFRELKQDFFADKDHFREISMGMSGDYTVAIE
                     EGSTVIRIGSIIFGGR"
BASE COUNT         3821 a         2937 c         2801 g         3526 t
ORIGIN      
        1 ccttcgaagg cgagtcctcc ctgatagtca aacctgtacc gtggtgttga ctatcaggga
       61 ggactcgcct tcgaaggagc gacagtcaag ggccggggag gcaataatat gccggcacga
      121 ataactgtgt acaaggacga taccctcaga atcaccgtaa aaacacctga attcatattc
      181 accaaagccg gattgacagg cccggaaacc tcaatggcca tttatatcgg caaggattcc
      241 atttatcatt ccaacctgtc attttcttac aacgcggaac agcatcaggt taactttttc
      301 aggggaaaca atcctatctc cagaagtccg tcattcaatt catttcacat gctcgacatg
      361 tattttgaat acctctcctg gaatctggac gattcaaaaa tcgtcttttc acgctcaagg
      421 ggaagctcga taggtaaagc acactttgaa tctgcttcat tctttgattc cagattgttc
      481 atggatctgg ccgggataga tgaatatcat ccgctgatgc agttaaagaa attctcggaa
      541 tggtattact ccgaaacctt tcccgttgcg gaatttgccg catggctgaa caggcctgcc
      601 gaagccgtga caggtttatg cattgacctt gccaacaggg gatttctgtt ctacgaccgg
      661 acatacaatg aggttaccct caagaagaaa gtgaatgatt ttctaaccgc ctttgcaaag
      721 aaaaaggatt acgatattct tcgtttcaac agcgagacaa aggcgccgct tgataatgcg
      781 gtactggata ccaaaaataa tcagatgaca gtcaacggag tatcaaatgt tcacctgagc
      841 gattctcaaa aggtggcaat atttccctac aaccagcaaa tcagaatcgg caggaaccgg
      901 gatatggatt ttgacggtgt tgtcgaagcg ggactcttta caatcttcgg acacaatttc
      961 agattcagct atgatacctt caaaataaac ctggcaaata ttgattcgat aagaattgct
     1021 gttgagattg atgaagtgga tgcttacgga aatcccctgg taaaggcaat ggataatctc
     1081 ctggaactga caaatgcaga tcttttcata gatgatccgg ataataagtc aggactgaaa
     1141 aaccttacac agtacccgat ggtaggggtg aagaaagact cctacatatt ttatgacaac
     1201 atacccgggc tcgagaatgt gtatccgagg gaggattttt atttcaggat cttccccttt
     1261 gtctatgaga acattgatca tttcaagatt gaaaccatca atcttccggg tgaatttgcg
     1321 ggaggaaata tcctcaaacc catgcggcag aatctgatcg tgaaagacag cagtcttctg
     1381 ggctttgaca tgataatacc ccccgaaggc attgaactgt acggaggcaa ggctgtcctg
     1441 tacgacagca taagcatggg cagcaaggga ctgcggggga aaggaaccat caaacatctt
     1501 gcatccacca ttgtttcgga acaattttat ctctatcccg attcaaccat tacccgggct
     1561 acttcattca gcatgggcgg agatgtgtcg ggaaggtttc cgagcctaga aagccataat
     1621 gttgcaatga gatggcttac aggggaaaat gaaatgcagg cagtcagcag caggggtaac
     1681 agcttcagga tgtttgacaa cagcagtgat tttgacggga acattgcata tacgccaaaa
     1741 tcaatgacag gtaccggaat atttaatacc agcgattcaa gaataacatc agacggtttc
     1801 agcttcacat ccaccagcgt aaaaacggat acgtcggatt tctttcttaa atcgccgtcc
     1861 acgggaggtt atgctttcat tgctgaaaat gcggccgtaa atgttgattt cacacttaaa
     1921 accaccgctc tacggctgaa tacagatgat tcattcataa aatttcctga aatccagtac
     1981 ataagtaaac tgagtgattt tgaatatgat atgcaggaca gaatactgag aatgcaggaa
     2041 aagagaaagg cggaaaagga actggttact cctgacaggc tgttaagaac ggatttgaaa
     2101 aatccggaaa aacccaattt ctttgccacc agcactataa gggacactat cagtttcaat
     2161 gcacagaagg caagttataa tcttgacggc gaatatattg aagcagaaaa tataaactac
     2221 atcaggattg cggatgcgct tatccagcct gaaaaaggca ggataaaaat cggccgcagg
     2281 gcaatgattg atccgctgca gaacgctgtt gtggcgatta acaaccgcca cctccttcat
     2341 tctgcaggaa taaatattga atcggcaaga aaatactcag gccggggaat atatgattac
     2401 actgacgaca gcggggaagt cagaaacatc agtttttccg aggtgactgt tgataccatg
     2461 gccacaagtg caaggggctt catcaccagc ggtgagaact tcatgctgag ccctgcattt
     2521 accttcacgg gcgatgtgaa cctgtattcc ggtcaggaat ttttactgtt cacgggagca
     2581 gcgggtatcg tcaacacatg cccgtcagtg aaatcagctc cggtacagtt caaatccttc
     2641 atcgacccga gaaacgtaat gattccggca agtgacaagc cgcgtgacat aaacggtaat
     2701 cccgtcatga caggttctct tttaaatatt gatgcaatga ccatctatcc ggctttcctg
     2761 tcggaacaga gatcctggat cgacgtggga ctggtcagtt caagaggata tctctattac
     2821 gacaaggcaa aaagcagtta ccttctgact tcgcttgaaa aaattgctga ccggacactt
     2881 cacggagatc tgatagcact cgacagaaac agctgcatac tgtcgggtga aggccggatc
     2941 aatctcggag ccaggtttga cccggtaaaa atgaccggag ccggacaaat aaaacataat
     3001 atcgacacgg gttatgttga aatacaggca ctgctgggat ttgattttca tttttcaccg
     3061 gaagccctca gaataatgag tcaggaattg aaattcattc cgactttaaa acctgttaac
     3121 ctgaactccg agctctacac aaagggaatg agggacctgt ttggagtgga aacggcgaac
     3181 aggatgaaag aggaccttga tctcttcggg gtttcaggtg aactgccccc tgaattcacc
     3241 ttcagactct ttttaaatga tgtgaccctg tactggcatg aggcatctgc atctttcagg
     3301 tcgaagggca gaataggact tggattcatc ggtacagatc cggtgaatgt atatgttgac
     3361 ggaatgattg agatacagcg ccggagagcc ggggacatgt ttgatatcta cctgaaagcc
     3421 aatgaatcaa cctggtatta tttttcctat atcaagggga atatgatgac cctttcatcc
     3481 aattcggctt acaattcggt aatagaaaac accaggcaga gactgcgcag gattcccaaa
     3541 aaggtatcca gggaacctta ttcatatatg attgcagtaa gggacaggat gatcagattt
     3601 ctgcgcagaa tggagagcag cgatgaagag gaacgctctc ctgcccagga tgatcctctc
     3661 aggaatcttg tcagatgata attcatggat tattttttgg ataaacagca ggcataaaat
     3721 gcagatcttt tatgcacctg atatcagtga tgatatctac atactcgacg aaaaggaatc
     3781 ccggcattcc gtcagggtac tcagattgaa gaaaggcgac cctgcaggaa tcattgacgg
     3841 aaagggcaat ctgtaccagg gaacaatcat tgatccggat gtaaaacatt gttcagtaag
     3901 gatactgaat aaaatagaga actatgaaaa aaggaattat tcgcttcata ttgccatttc
     3961 acctctcagg aatcccggcc ggctggaatg gtttacagaa aaatgtgtcg aaataggaac
     4021 cgacgagatc actcccctga tttgcacgaa caccgaaaag cacagtataa agaaggacag
     4081 gctgcagaat ctgatcatat cagccatgaa gcagtctctc aaggcaaagc tgacaaagct
     4141 caatgatcct tccgggttta atgacttcat cagcatgaaa aggaagggga agcttataat
     4201 agctcactgc aacagcagat tcagccgtga ttccataaaa gatgtttacg gaaagggtga
     4261 agatgctgtt atcctgattg gtccggaggg tgatttttct gaggaagaga ttcttgcagc
     4321 aacagaaaga gattattgtc ctgttcacct gggtccttcc aggctgagga cggaaacggc
     4381 aggtattgct gcctgccatt cagtatatct tatcaaccag taaaaagccg gatcctaggg
     4441 ttacttcttc tccccggcgg gaggagagtg atctgccggc ttccatcttc cagccttgcg
     4501 caaagcctga tcaagcagat attctatctg accgtttgta ctgcggaatt catcagcagc
     4561 ccatttttca actgccttca gttttcccgg atcaagcctc aggacaaatg cctttttccc
     4621 actcatcatc agttatacaa ggttcctgta tttaccaccg gggtggtatc cttatcactg
     4681 cagagaacca ccatcagatt gcttaccata gcagcttttc tttcctcatc cagatcaaca
     4741 atcattttgt cggaaagctg ctcaagagcc atctgaacca ttgaaaccgc accctccact
     4801 atcttgtgcc tggcagatac caccgccgca gcctgctgcc gcctgagcat tgcattggct
     4861 atttcctccg aataggcaat ataatttatt ctggcttcaa taacctcaac cccggcaatc
     4921 ttcaatctat cgcgtatctc attctcaagc tcatcattga tctcttcacc cccgctgcga
     4981 agagcaatct cctcaacatt atcatcaaaa ttgtcatagg ggtatagacc ggccagtttc
     5041 ctgagtgctg catcactttg taccagaaca aaattcttaa agtcatctac atcaaaaaca
     5101 gccttgtagg tatccaggac cctccatacg agaacaaagc caatctttat aggattcccc
     5161 ctcttatcat tgaccttgat tggttcactg tcaaaattac gggctcgcaa agagatttta
     5221 cgcttcgtgt aaaagggatt gacccagaaa aatccattct cccttatcgt acccttatag
     5281 gcaccgaata gaaccataac acaggatccg ttcggataaa caatggtgta tccaaacact
     5341 gcaagaacga aaagaattgc cagaattgag ccaagccaga ttatttcaaa ggcaaatccg
     5401 acaattgcac cggccagaaa tattgccgca aagggcagag atacatagcc ggaaacaggt
     5461 ttgactgaat attcatgttt catctcttgg atatgatatt aaaataatat tataaatata
     5521 tcattttgat tttaaataat aaaaaaaaag acacagaaaa atttctgtgt cttcatgttt
     5581 ttttcagtgt ttcctgatcc ttgtaatcag ccggcgtgta agattttttg gaggctcagg
     5641 cttaagcatg ttaagatatc cgatcacatc atcaaactgc tgatacagac caaaagcatc
     5701 ctcatgcagg tcaatatccc gggttttgat ttcctgatat ttaacaaatt gagtagaaat
     5761 taaatccata ggcttcatgt tttccgtttt tctgaaaacg ccgtatggat aacaatattg
     5821 cagtatgtgg gaaatatatt ttaaaagctt gtgacacctt aatttaaggt gaggctgatc
     5881 ttcttttcct ccataatttt ccgcatattg atcagggcat acctcatcct gccaagtgcg
     5941 gtattaatgc tcacatttgt tatatccgca atctccttaa agctcagtcc ggcgtaatgc
     6001 cgcaatatca ccacctctct ctggtcatcc ggaaggcagc tgatcatttt cctcacatcc
     6061 ttctggatct gccgtctgat gagattatcc tcaatggttg aatcggacag ttttctggaa
     6121 ttaaaaaggt cggattcata ttcattattt gatattgtat tcatttgttt tgccctcctg
     6181 aagtggtcaa ttatcagatt atgggcaatc cgcattaccc atgatacgaa tttgccgtta
     6241 tcctggtacc tgcccttttt aacagactgt atcaccttca tgaaagtatc ctggaaaata
     6301 tcttcggcaa gtgcctgatc gcgtatataa agacttatat atgcaaagac cttgttttta
     6361 tggcggtgga ttatctgatt gaaacaggac tgctccccct ttacgaatct ctgaataagt
     6421 tcgtaatcgg aaattgtttt actcatagct cactctttta gaatcaagag tagagttgtt
     6481 ttataggcag tcctcctgtt tttttaaatt atttattgca aattaaatca aattttttta
     6541 atctgaaagt attatttttg cattttaata aaaactttta cttataactg ctgaatccat
     6601 taatgaacgg ttgcattgaa atcagggggg caagggttca taatctcaag aatataagct
     6661 taaaaatacc aaggaacaaa ctggttgtca taaccggtgt ttcaggatca ggaaagtcat
     6721 cacttgcctt tgacaccatc tatgctgaag gccagagaag atatgtcgag agtctctctt
     6781 cctatgcccg ccagtttctt gggaggatga acaaacccga agttgatttc atcagcggaa
     6841 ttcctcctgc aattgccata gaacagaaga tcaaatcccg gaacccaagg tcaactgttg
     6901 gcacttcaac tgaaatttat gactacctga gactcttata cggaaggata ggccgcactg
     6961 tttcaccggt ttccggcaag gaagtcaaaa agcacagtgt tgacgatatt gttgaattcc
     7021 tggcatcact tcaggaagac agcagggtta taattaccgc tgcagggcaa atccctgaga
     7081 acatcaatat cggggattat ttttcctttc tacgcaaaca gggatttgac cgcatagaaa
     7141 ccgggggcaa aataatcagg atatcagaaa taaatgaaga tgatttcaaa aacggccaga
     7201 aaatcaatat tgtgattgac aggctgatca ttgatcattc acctgacaat ttcagcaggg
     7261 cggcagattc tgcgcagact gcatttcata ccggccaggg ctattgtgaa gttcttatcc
     7321 tgaatgatga cggtccgcct gacagggaag gtttctcgac cagattcgag gctgacggca
     7381 ttgttttcga ggaaccgtcc gaatacctgt tcagtttcaa taaccccctg ggggcctgcc
     7441 ctctgtgtga aggttacgga aggataatag gaattgatga agatcttgta atacccgacc
     7501 ggaatctttc ggtttatgat gatgccattg cctgctggaa gggagaatca atgaggagat
     7561 ggaaggaaag gctgatctcc agcgcccatc tctttgattt tccggtacac aagccttatt
     7621 ttcagctttc tgatgaacag aaggcgcttt tatggaaggg caacaggtat ttttacggcc
     7681 tcgacagatt tttcagatat cttgaagaaa agaaatacaa gattcagtat cgtgtaatgc
     7741 tgagccgcta caggggcaag actgtctgtc ctgaatgcag gggatcaaga ctgcgaaagg
     7801 aagccggata cgttaaaatc gccggaaaaa ccattcagga gctggcagat atgccggcaa
     7861 acaggctgta tgatcatttt tcggaaataa aactcgattc acccggcgaa gaaatggttg
     7921 ccggaagatt actgacggaa ataaacacga ggcttcgttt cattcttgac gtcggacttt
     7981 cctaccttac cctcaacagg ctttcctcca ctctctccgg cggtgaatcc cagagaataa
     8041 acctgtcatc ctcacttggc agcaaccttg tcggctcaat gtatattctg gatgaaccca
     8101 gcataggact tcatccccgc gacaccgctc tcctggtaag ggttctcagg gaactgagag
     8161 acctgggcaa cacggtaatc gtggttgaac atgaggagga aataataaag gcagcagatc
     8221 atataattga tcttggtccc gaagccggaa ggctcggcgg agaaattgta tttcagggaa
     8281 gtccggccat ggcggcttcc ggcggatctc tgactgcagg atacctggcc ggaacaatga
     8341 agattgccgt cccctcttca agaaggaagt ggaacagttt tatagaaatc gtcggggcaa
     8401 gggaaaataa tcttaaaaat attgatgtaa agtttcccct gcacactatc acggccgtga
     8461 ccggagtcag cggatccggc aaatcaagcc ttgtaactga aatccttcac aaatacctgt
     8521 ccaacaggat cacaggaagc aatctgcagc caggtcagtt cagggaaatc agaggcgaca
     8581 taaccggtct gaccggcatt gaactgatag accagaaccc gataggcaaa tcaagcagat
     8641 caaatccggt aacctatctg aaggcatatg atgaaataag aaagctgttt gccggacaac
     8701 aggcttcggt acagaataat tacaagcctt ctcatttctc cttcaatatt aagggtggaa
     8761 gatgcgagga atgccagggt gagggcataa taagagtaga aatgcagttt atggctccgg
     8821 ttgaactcac ctgcgaggaa tgcaggggaa tgcgtttcaa aagagacatc ctggaagtga
     8881 aataccacgg aaaaaatatt cacgaccttc ttgaaatgac aattgatgag gcgatagaat
     8941 ttttttccgg acacccgggt aaaactgaaa gtaaaattgt tgaaaaactg aagccgcttt
     9001 cggaagtcgg actgggatat attaaaatgg gtcagtcgtc cagtaccctt tcaggcggag
     9061 aaagccagag agtcaagctg gcttatttcc tcagccagga aaaagcttcc ggacatatcc
     9121 tttttatttt tgatgaacca acaacaggtc ttcatttcca tgacataaac aagcttctta
     9181 aatccataaa cgcccttgtt gaacacggcc attccgttgt tgtaattgaa cataacccgg
     9241 agataatcaa aagctgcgac tggattattg atcttggacc cgaaggcggg aatgagggtg
     9301 gttatgtggt atttgaaggg aaaccggaag acctggtgaa ctgtgccaaa tcatacactg
     9361 gaaaatatct cctgcacaat ctggaaaaga acagcaaggc tccgctgaaa acgggtcacc
     9421 gctgacaaca ataaacaccc tgattttatt tcttcagcct gaacccgaac ataattgcaa
     9481 cgcatccgct tgacggctgc tgcccggaac gggttgcaga gggcaccttg ataaggaact
     9541 tgagctgctg gctcgattca agtttaaaat cccaggtact ggaatagttt ttatccttgt
     9601 tggtataaag tacattttta tttgaatcca gaaccctgaa ttcaatatcc ggaagttttg
     9661 agtcgcccac aatggaaacc ctgtaattca tatcggaata gaaggtctta tacaattcgg
     9721 cctcttcgcc ttcaaccagg acggcagcgt gataattgcc gtcatgtgta tagccggcaa
     9781 ggttcgggag acattctttt ttggcaaatg ccttgcactg tgcacccgca aatcccccgg
     9841 aaaaaaggag caggatgacc ggaagaagaa tatttttaag tgttttcatg ttcttttaga
     9901 ttacaaatga attcctgatc tctttaacaa ccgctgacaa ttccaaaaaa acagcaggtg
     9961 tcatgtccgt ccgggtttcc gatttcaata cggttgatct ggagtccggg tcatattccg
    10021 gccttacagg cgaagtgtca agcctgattt tatcaaatac gttcttaagt ttttcaactt
    10081 ccaccatcag ttcatcaacc gcagcagtgc ccttactacc gcttagaagc ctgagaagaa
    10141 tatctacaga gagcttctgg tcaattatcc tgccaaccag tttgtttccg tcgaaatcat
    10201 tcatgtcaac cagctgggtg gagatataga gtccctcaat ccatcccccg gtaagaacca
    10261 tagctgctgt tgcaggctgg ccgttatcct ccagatagga attggagttc atgaatgtct
    10321 gggatactat atccattatt acttcactgt tgttgatatt ttcctcaagc ctgtcaatat
    10381 ctttctgttc aatagccttt aaaataccca gtccgtctgc cattttcttg gccgcattca
    10441 tgtaatcgat cagtaattgt gtctgttcgt aaaggcttgc aaagctaaga tcgcaggtat
    10501 aaattccaag gttcagtgcc atggacttgt ttgtaacata tttattgaca ttggatacgg
    10561 gattcagcaa tttctcctca aatcttgcac ctgccgattt tataagcatc gccgattcaa
    10621 gaggagatgg cagcgcctga aaaatctttt ctgcctgttt aatgtcctgt attacagctt
    10681 ccttatccct gatatcgagc tccagattca cttcgggagc ttttctgcca cccttacagc
    10741 aggtaaacag aattatgccc agtataaata caataccaat tccgattccg gaactaattc
    10801 tgaactgtct agtcatgatt tttatcaatt aaattgcaaa cattaaaaca ggactaaatt
    10861 agcattttat ttttatttat caaaaaggcc ttaatgatat tgatggtatt tctacctttg
    10921 aactgaaatt aaaatacaga ctttaccggg gtgaaaaaat cggagaaaag atacagggaa
    10981 cttgaaggca ttctcaagaa gggcagggta tcgctgataa gcgaggcaat ccggtcgctc
    11041 cgtgaggagg agccctttga gggagcaata gaactgcttg cagaacatta caatgtcact
    11101 gatgataaaa tgaatatcat tgcgattgaa gaatttttca acgacatcaa atacctttca
    11161 gcccgttccg aaatcatggc agaaatacgc aagccttcgt acaagcagaa aaccataagc
    11221 atgctggttt catcatgctg gcagtcagga ctggattatt cggagtatgt tcaggacata
    11281 gtcagggtat ttctcgaagg ggattacact acagccattg aatgcatgac actgattgaa
    11341 gagtcattac gctacaatac aagggaagat aaggataagg ccatacacct ggtggaaaga
    11401 agcccggagg cttttacgca tgaaaaagac ccgctttcca gggagcttat ttccatactg
    11461 gcacgctgag cctcttcatc tgtcttttac atctgaccct ctgaaccgcc ggagtttaaa
    11521 aggctgaaga attctaaaaa tcctaagatt cctgcatcag tcttccgcag caaaatattt
    11581 catgaactgg gtccgctcat aaacaacagg cctgccatag ttccggtaat taagcctgcc
    11641 tctgaattca tccactgatc tgaattcctt cctttccatc cattcttcga tctgtgtatt
    11701 cataaccttc agaaactgca gccccttgtt gtaaagaacc gagcaaacct gtaccgagtt
    11761 tgcgcctgcc agtatgtatt ttattgcatg atggccgctg tgaacacctg ttgaggctga
    11821 aatatcaatc ttaatatcct gtgcactggc catgcctatc cacctgagaa catatctcat
    11881 ctcttcctcc ctgctgaaga ccggagccgg tacaatatcc attttgtcaa catcaatatc
    11941 cggttcataa aacctgttga acatcactat cccttcaacg cctctcatat agaactgatc
    12001 aataatattc aggatgtttg aaaagcggaa tccgattttc agcgcaatcg gtattttaat
    12061 ggttttcttc agcttctcca ctagttccag gtagactttt tcggaatcgg tagcgctttt
    12121 tttcctgtca tccggaagga aaaacacgtt tatttcaatt gcatcggcac cggcttccgc
    12181 gatattcccg gcaaagcctg tccagccacc cgaggaataa cagtttatac tgggtatcac
    12241 cggtattgaa agactttcct ttgcgctttt aatgagactg aggtattcat caaccctgtt
    12301 tgacctcata taatatgaca catagtcacc tgcctccgga tagtcagcac taaccggaat
    12361 ggtatgaata taatggtcaa tctgttcctc gaaaagggat ttcaggacca cggcacctgc
    12421 accattatcc tcggcttcct tcagatgatc cagactggag gttaatctgc tgctgctcac
    12481 tataagagga cttttaagat caagccccag atattttact gacaatttac ccatttattg
    12541 aatcttttaa ttttctatcg ccctccaaat ataatacttc caattcttat taccgtgctt
    12601 ccttcctcaa tcgcaactgt gtaatcacct gacattccca tggaaatttc tctgaaatga
    12661 tccttgtctg caaagaaatc ctgcttcagc tccctgaaac aagctgccag atatctgaat
    12721 tcatccctca cctgttccat atcacttgta aacgtggcca tccccatcac tccgcagatc
    12781 cttactgctt ccatccgtct gacttcatca gagcctgcca gctccctgac ttctttcaaa
    12841 gcaaaaccat attttgtttc ttcctcggct atgtggaact gcagaagaca atcaattctc
    12901 ctgttgattt ttaaggctgc agtgttaacg gctctcagaa gtttaaatga atccactgac
    12961 tgtatcatgc tgacaaaggg aaccacatac tttaccttgt tggtctgcaa atgtccaatc
    13021 aggtgccatt caatatcacc ggggagcagg tctttctttg acaacagctc ctgcaccctg
    13081 ttctc
//
LOCUS       DAOSPX010000056        12941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4841_length_12941_cov_4.495189, whole genome shotgun sequence.
ACCESSION   DAOSPX010000056 DAOSPX010000000
VERSION     DAOSPX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12941
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4841_length_12941_cov_4.495189"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLZ75_04440"
     CDS             <1..209
                     /locus_tag="PLZ75_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71680.1"
                     /translation="QYFGVEMLDQAFAKEGYKTDCTELNRKEYFHQKVLGPGQLHELD
                     AARTIAAFFPFLLRTEENMTAEKQ"
     gene            complement(227..499)
                     /locus_tag="PLZ75_04445"
     CDS             complement(227..499)
                     /locus_tag="PLZ75_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS71681.1"
                     /translation="MTRKLKTIIIDEEPNSVDFIDSLILRYCPELEIAGKAYDVSEGV
                     RVVKNSKPDLIFLDVEMPNGTAFDLLSHFPNEKGAGQAPCSNLKPG"
     gene            complement(1021..1551)
                     /locus_tag="PLZ75_04450"
     CDS             complement(1021..1551)
                     /locus_tag="PLZ75_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71682.1"
                     /translation="MKNGQTHTSVMNYNTVTEKMVFIKDDSYFDLTNTGMIDTIILQD
                     IRFVPVGKAFYEVLISDSSSLYIQYKGELLPAGKPVGYGGTSHLASSTYLSSVDLSGG
                     RYNLPVPPEFTVNMYSVYWIRKGTEWSGFVNEKQFLKLFPEKAGALKAFIKKNRIKID
                     RKKQMIELMEYCNSLN"
     gene            complement(1712..3826)
                     /locus_tag="PLZ75_04455"
     CDS             complement(1712..3826)
                     /locus_tag="PLZ75_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71683.1"
                     /translation="MKALIPGFALTLAVLISCGNDQKINRYQLVTRHNIETSAIDSLN
                     SLSVGNGEFAFTVDVTGLQTFPAFYRGGIPLGTMSNWGWHSSENPQNYSLEDNYREYE
                     VNGRQVKYLWQYGSEDEERKKLASTWLRENPHRIHLGMIGLYILKKDGTEITIDDISD
                     NVQRLNLWTGEIVSRFTVDGFPVQVTTLCHPELDQVSARIESGLIAENRLGIRVHFPL
                     GIPQPAGYDFENPEGHVTAIRASSANVTLFERIQDADRYFVKLSHPGGSIENAAPHLY
                     YIFPRNDSKVFEFSCLFSKRDEHGSPEDFAISAGKNRKSWEEFWSTGAAVDFSQCTDP
                     RAPELERRVILSRYLTRIQCSGSLPPAETGLTYNSWFGKFHLEMHWWHAVHFALWQKE
                     AILARQMNYYSDIIGEAGETARIQGYRGARWPKMTNPSGRESPSGVGVFLIWQQPHPI
                     FYSELLYKNSSDKNTVLGKYSEIVFESAEFMASYAFHDRVNSRYVLGPVLIPAQESLK
                     KESTLNPAFEIVYWYWGLKTAAEWKKRLGQEIPALWTDITERLSELAVQDGLYLCTED
                     TRDSWINPRYMSDHPIVSGIMGILPETGKVNSLILGNTLDTIMKKWNWPSGWGWDFPM
                     LAMSAASIGRGEQALDFLMMDAPKNRYLLNGHNYQDPRLAVYLPGNGGLLTAVAKMCV
                     CGQFPQNGRWKVRWENLNEYVD"
     gene            3984..5105
                     /locus_tag="PLZ75_04460"
     CDS             3984..5105
                     /locus_tag="PLZ75_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOS71684.1"
                     /translation="MDTAITIIGAGVIGLAAGERISENFSNVFIIEKHPSFGRETSSR
                     NSEVVHAGIYYPEGSLKAVLCVEGRELLYDYCIKHEIPYNRCGKLIVATNREEVKVIR
                     DIKETARRNGVKLEILDKDQIAALEPEIFAVNALYSPDTGIVDTHSLMKSLETGIIHN
                     GGQMVYGSEVTGIRKLDKGYEIKLMDADGQEFCFTTGIVINSAGLYADRIAAMAGIND
                     ENLAIHFCKGEYFRLKPPRNRLISRLIYPVPYHNMEGIGIHATVDLAGGVKLGPDVTY
                     LDSNIQDYHVDVSKQEIFWRSAKKFLPFLSPDDLSPELAGIRPKTQKKGDPARDFYIA
                     EESARGLKGFINLIGMESPGLTSCLSIANYILNLVINIE"
     gene            5155..5988
                     /locus_tag="PLZ75_04465"
     CDS             5155..5988
                     /locus_tag="PLZ75_04465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS71685.1"
                     /translation="MKSQSKYKFPVLTLIWVLVPLVLAGQSNKSGEEYVPEVGQQGKD
                     VVWVPTPQELVDTMLSIAKVTPSDFVIDLGSGDGRTVISAAKRGARALGIEYNPDMVN
                     LSKRNAARAGVSGKAEFIEADLYDTDLSKATVITMFLLPEINLKLRPRLLELKPGTRI
                     VSNTFTMGEWEADVHVTTEDNWNSWNTALMWIVPAKAEGTWKLGRDELILTQEYQMLQ
                     GRLKQGEKTVSISGGRLKGNEITFRAGDYLYTGFINGNRMTGKVTDSVLGNTGDWTAV
                     R"
     gene            complement(6061..8082)
                     /gene="uvrB"
                     /locus_tag="PLZ75_04470"
     CDS             complement(6061..8082)
                     /gene="uvrB"
                     /locus_tag="PLZ75_04470"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOS71686.1"
                     /translation="MKFKLISDFVPTGDQPEAIRQLAEGLEKGVPYQTLLGVTGSGKT
                     FTIANVIEKAQRPVLVLSHNKTLAAQLYGEFRQFFPENRVEYFVSYYDYYQPEAYLPV
                     TDTYIEKDLSINEDIEKLRLSTTSALLSGRKDVLVVSSVSCLYGIGNPDDYHSNTVHL
                     ETGKKTGRNQFLRKLVDSLYSRNELEFRHGTFRVRGDTVDIFPAYADIAVRVIFFGDQ
                     VEEIYTFDPQSGDVFEKLDDYIIYPANIFVTTRDRINQAVGMIQDDLVKQVIYFNDIG
                     KKEEAKRLEQRVSYDLEMIKELGYCSGIENYSRYFDGRPAGTRPFCLLDYFPRDFITV
                     IDESHVSIPQIRGMYGGDHSRKQTLVEYGFRLPAALDNRPLRIEEFESLTGQVIYVSA
                     TPADYELEKSEGVFVDQVLRPTGLLDPPVEVRPTLNQIDHLMDEIRERALAGERTLVT
                     TITKRMAEELNAYFIKFDIKSRYIHSDIDTIERIEIMEGLRAGTFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSARSLTQTAGRAARNLNGKVIMYADTVTNSMQMTINET
                     DRRREKQLKYNAEMGITPARIVRKTGTILAGLSKKGVTVKAYIEPGPVDIAADPVIKY
                     MNREALEKAIEATRKKMEKAASELDFIEAARFRDEMNGLQKLLKGRPKQ"
     gene            complement(8252..9112)
                     /locus_tag="PLZ75_04475"
     CDS             complement(8252..9112)
                     /locus_tag="PLZ75_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOS71687.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEELISVEEEVELAQRIK
                     KGDRYALEKLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSRFEQENERAPTAEELAE
                     VLELPKEKVTDTLKVSGRHISVDAPFIEGEDNSLLDVLTNIDSPVADKLLMDESLSRE
                     IYRALATLTERERDIIRYFFGIGCQEMTLEEIGEKFGLTRERVRQIKEKAIRRLRHTS
                     RSKLLKGYLG"
     gene            complement(9380..10831)
                     /locus_tag="PLZ75_04480"
     CDS             complement(9380..10831)
                     /locus_tag="PLZ75_04480"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOS71688.1"
                     /translation="MKGRNLLGALLMAVLGAAIALFAYTRIADKPAVVVAKDSSRTEA
                     SEARAYLTALQDLPGQVDFTYAAEQTVHAVVHVRTKSMVQQPSNPIMEWFYGDRYRSR
                     PREVAGYGSGVIISSDGYIITNNHVIEDAENIEVKLNDNRVFSAELIGRDPSTDIALL
                     KIKAENLSFLKYGDSDKLKVGEWVLAVGNPFNLTSTVTAGIVSAKGRNLGILDNDYRI
                     ESFIQTDAALNMGNSGGALVDTRGLLVGITSAIYSPSGAYAGNSFAIPVSIVQKVVDD
                     LRQYGEVQRALIGVNITDVTSEIAEQKKLPEVKGAMITEVREGGSAADANLKKDDVII
                     RFDGVVVNNATELQEQVGKHRPGDRVSITFIRNGKENTASLTLKNISGTTDVVPRGSG
                     SGAVFGARFEPVSQADKRSYGINSGVKIVEIGDGLFRDLGIRRGTIITAVNNKKVNSS
                     DDVRSAAGTSERSLKSIAGITPEGREFHFQYGN"
     gene            11004..11783
                     /gene="dapF"
                     /locus_tag="PLZ75_04485"
     CDS             11004..11783
                     /gene="dapF"
                     /locus_tag="PLZ75_04485"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOS71689.1"
                     /translation="MMTVTFNKYQGAGNDFVIIDNRKNIINPDNSVLINRLCDRRFGI
                     GADGLILISEIKGHDYEMKYFNSDGKLGSMCGNGGRCAAHFAMKHGIAGKEQEFMAFD
                     GLHQAKVDNDTVRLQMADVSDHKLVDDNYFIDTGSPHYILFTDDVDSLNVYEEGKRIR
                     WSEKFAPGGTNVNFVQVIDNGLKIRTFERGVEDETLACGTGVTASAIASVLKGHFGTS
                     PVDVRARGGNLRVEFEIDNKMIRNIWLTGPATFVYEGKLDI"
     gene            11907..12389
                     /locus_tag="PLZ75_04490"
     CDS             11907..12389
                     /locus_tag="PLZ75_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71690.1"
                     /translation="MKAYTPIILSVFLLLPCKLNSQPGTTELMKAIEEISADIIREMI
                     SQGYVVVAAEYRKRSPAWQVKKYRGTPLLIHTNTNDEDVNVLEVEHLIKSFRAEGKSG
                     FSYEIFENMPGGHSFDRMDYKEAKEIRMKIYNHLNRYLKPPNPFRSVKDLQKAGYGFN
                     "
     gene            12426..>12941
                     /locus_tag="PLZ75_04495"
     CDS             12426..>12941
                     /locus_tag="PLZ75_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133828.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOS71691.1"
                     /translation="MSKEKLYGKTLDELVAVTKKAGLPGYSAKQIAGWLYGKEINTLD
                     EMTNLSKKMRERLSEEYEIGLSPPAGVTESRDGTKKYLFKVLSGKYIESAYIPEKNRA
                     TLCISSQAGCRMACIFCMTGRQLFQGNLSSNEILNQFRSIPEFGMITNIVYMGMGEPL
                     DNIGEVLKSLEI"
BASE COUNT         3542 a         2995 c         2806 g         3598 t
ORIGIN      
        1 ctcagtattt cggagttgag atgcttgacc aggcctttgc aaaggagggt tataaaacag
       61 attgcacgga actgaacagg aaggaatatt tccatcagaa ggttctgggg ccggggcagt
      121 tgcatgaact ggatgccgcc cggaccattg ccgcgttttt tcctttcctc ctgaggacgg
      181 aagaaaacat gactgctgaa aaacagtgag catttccgga ggcgcttcag ccgggtttta
      241 ggtttgaaca aggggcctgt ccggcccctt tttcatttgg aaaatggctg agcaaatcaa
      301 aggcggtgcc attcggcatt tcgacatcca ggaaaatcag atccggtttg ctgtttttaa
      361 caactcttac cccttctgat acatcatagg cttttcctgc aatttccagt tcagggcaat
      421 acctcagaat gagcgaatca ataaaatcaa cagaattcgg ttcctcatca ataattattg
      481 ttttaagctt ccttgtcatg tcatgatggg aatttcgatt tcaacacggg tgccttcgga
      541 atcagcccgt ggcagattga attctcaacg tatggctgaa taagcattga tggtacgttg
      601 tatatcaaag gatcaatttc tgacatgaaa acttcgtaag gtatgtgttt gtctcaagtt
      661 tatcatgacg gtacatatag tactgtatcg aattcagggt gttgaaaata aaatgaggat
      721 tcatctggtg tctcagatta gcctgtgtga gttcctatat tgcccaggga attggaagca
      781 ctggcaagct gtttagccgg aatacagtaa tcaagtgatt tatgataata atacagggag
      841 gaatcatata atttctcaag ataaaaattc ctataaaatt aaaaaaacac cggcagatga
      901 ccagtattag tttccagatt gcaggtatga accaacaaat ggttttcctt aaaagcgctt
      961 aatcattaaa tccattaaga atttgtctga taaagcagtc gatagattaa gaatcaggtt
     1021 ttaatttaaa ctgttacaat actccattag ttcaatcatc tgttttttcc tgtcaatttt
     1081 tatacggttt ttctttatga aagctttcag tgctccggct ttttcgggga agagtttcag
     1141 aaattgtttt tcattgacaa aaccggacca ttctgtacct tttcttatcc agtaaactga
     1201 atacatgttc acggtaaatt caggtggaac aggcaaatta tatcttccac ctgaaagatc
     1261 gacactgctg agataagtac ttgatgccag atgggaagtt cctccgtatc ccacaggttt
     1321 gcctgccggc aataattcac ccttgtattg aatatataat gaggaggaat cagaaatcag
     1381 gacttcataa aaagccttcc cgaccggaac aaacctgatg tcctgcagaa ttatcgtatc
     1441 gatcataccg gtatttgtaa gatcaaaata actgtcatcc ttaataaaaa ccattttttc
     1501 agtaacagta ttataattca taaccgatgt gtgagtctgt ccattcttca tgaaaattct
     1561 gcctgcagaa aactccggaa aatgatattg cgtcatggat ccatcactgg tttgctgtcc
     1621 ctgtaataat atcgtcataa aattgaatat cagggcgata attgataaag ataatttcct
     1681 tttaatcata tatttgtgat tttgtttatc atcagtcaac atattcattt aagttttccc
     1741 atctcacctt ccatctgccg ttctgaggaa actggccgca gacacacatt tttgcaacag
     1801 cagtcagaag tcccccgttt ccggggagat agactgccag ccggggatcc tggtaattat
     1861 gtccgttcag cagatatctg tttttagggg catccatcat aagaaaatcc agagcctgtt
     1921 ctccgcgccc gatggaagcc gctgacattg ccagcatggg gaagtcccat ccccagccgg
     1981 aaggccagtt ccattttttc attatggtat ccagtgtgtt tcccagaata agactgttga
     2041 ctttgcctgt ttccggcaat attcccatga ttccggaaac tatggggtga tcactcatat
     2101 atcgcggatt gatccaggaa tccctggtat cttccgtaca aagatagagg ccatcctgta
     2161 cagcaagctc cgaaagtctt tcagtgatat ccgtccataa agcaggaatt tcctggccca
     2221 gacgcttttt ccattctgcc gccgttttca gtccccagta ccagtaaacg atttcgaatg
     2281 caggatttaa agtgctttcc tttttcaggc tttcctgtgc gggaatcagc accggtccga
     2341 ggacatagcg gctgttaacc ctgtcatgga aggcatagga tgccatgaat tcggccgact
     2401 caaaaacaat ttcactgtat ttcccgagaa ctgtattctt atcggatgaa tttttgtaaa
     2461 gcagttccga gtaaaaaatc ggatggggct gctgccagat gagaaaaaca cccacaccac
     2521 tggggctttc cctgcccgaa ggatttgtca tcttgggcca ccgtgcaccc ctgtatccct
     2581 ggatccgggc agtttccccg gcttcgccga tgatatcaga ataataattc atttgcctgg
     2641 ccagaattgc ttccttttgc cagagcgcaa aatgaacggc gtgccaccag tgcatttcaa
     2701 gatgaaattt cccgaaccag ctgttataag tgagaccggt ttctgcggga ggcaatgatc
     2761 cgctgcactg tatccgcgta agatagcgcg aaagaatgac ccttctctcc agttcaggcg
     2821 ccctcgggtc agtacattga gaaaagtcaa ctgcagcccc ggtactccag aactcttccc
     2881 agctcttcct gtttttcccg gcgctaattg caaaatcctc aggagatccg tgttcatccc
     2941 tcttactgaa cagacatgaa aattcaaata ccttactgtc gtttcgcgga aaaatgtaat
     3001 aaagatgcgg tgctgcattt tcaattgatc ctccgggatg gctcagttta acaaaatacc
     3061 tgtcagcatc ctggatcctt tcaaacaatg tcacattggc ggaagaggca cgtatcgccg
     3121 tgacatgccc ctcaggattc tcaaaatcgt atccggccgg ctggggaatt cccaatggga
     3181 aatgtacccg tattccaagt ctgttttcag ctataagtcc ggattcaatc cttgcagata
     3241 cctggtccag ttccggatgg cacagggtag tgacctgaac aggaaaacca tcaactgtaa
     3301 aacggctgac tatctcaccg gtccacagat tcagtctctg aacattgtcg ctgatatcat
     3361 ctatggtgat ttctgtcccg tcctttttca ggatatataa gcctatcatg cccaaatgaa
     3421 tacggtgtgg attttctctc agccatgtgc tggccagctt ttttctttcc tcatcctcgc
     3481 tgccatactg ccagagatat ttcacctgcc tgccatttac ttcatattca cggtaattat
     3541 cttcaagact atagttttga ggattctcag atgaatgcca gccccagttc gacatggttc
     3601 ccaggggtat tccccccctg taaaaagcgg ggaaagtctg aaggcccgtt acgtcaacag
     3661 taaatgcaaa ttcaccattt cctacagaaa gtgaattcag actgtctata gcgctggtct
     3721 caatattatg ccttgtcacc agttggtatc tgttaatttt ctgatcattg ccgcatgaaa
     3781 tgagaactgc cagtgtcaga gcaaatcccg ggataagtgc tttcatgtta aaaagttatt
     3841 tggaacataa aagtactggt ttgattgaaa tctgccaaga ttgtccctca gcaatgaatc
     3901 attcccgtaa caaatttccc ggatgatgac ggcatatcgc tttttaggct atttttggaa
     3961 aataaatttg tcagcaattc actttggata ctgccattac catcattggt gcaggagtaa
     4021 taggtcttgc cgccggggaa aggatttcgg aaaatttcag caatgttttc ataatcgaga
     4081 aacatccgtc attcggccgg gaaaccagca gccggaacag tgaggttgtt catgccggaa
     4141 tttattatcc ggagggaagc ctgaaggccg tgctgtgcgt ggaaggcagg gaactccttt
     4201 atgattactg cattaaacat gaaataccgt ataacaggtg cgggaaactg attgtcgcca
     4261 caaaccggga agaagtaaag gtaataaggg atataaaaga aactgcccgc agaaatggag
     4321 ttaagctcga aatacttgac aaagatcaga tagctgcgct cgagccggaa atttttgcag
     4381 taaatgccct ctattcaccg gatacgggaa ttgttgacac acattccctg atgaaaagcc
     4441 ttgaaaccgg gataatacac aacggcggac aaatggttta tggaagtgag gtgacaggga
     4501 tcaggaagct tgacaagggt tatgaaataa agcttatgga tgctgacgga caggaattct
     4561 gttttacaac aggaatagta ataaattccg caggccttta tgcggacagg attgccgcaa
     4621 tggccgggat caatgacgaa aaccttgcca tacatttctg caagggtgaa tatttcaggc
     4681 ttaagccgcc ccggaacaga cttataagcc ggctcatcta tccggttcca tatcataata
     4741 tggaaggcat aggtatccac gcaaccgttg atctggcggg aggtgtaaag ctcggaccgg
     4801 atgtaacgta cctcgactca aacatacagg attaccatgt tgatgtttca aaacaggaaa
     4861 ttttctggag gtctgcaaaa aagtttctcc cttttttgtc tcctgatgac ctgtctcccg
     4921 agctggcggg aataaggccc aaaacccaga aaaaaggaga tccggcaaga gatttctaca
     4981 ttgcggagga aagtgcaagg ggattaaagg gatttatcaa tcttatcggt atggaatcgc
     5041 ccggactgac atcctgtctt tccattgcaa attatatcct gaatcttgtt atcaatattg
     5101 aataaaacac ttatttttac ctgattttcc agcacagaaa caaaagagga tgaaatgaaa
     5161 tcacagagca aatacaaatt tccggttttg accctgatat gggttcttgt acccctggta
     5221 ctggcagggc agagcaataa atcgggcgag gaatatgtac cggaagtagg ccagcagggc
     5281 aaggatgtgg tctgggttcc cacaccacag gaacttgttg atacaatgct ttcaatagca
     5341 aaagtaactc cttctgattt tgtcattgac cttggttcgg gggatggcag aacagttata
     5401 tctgcggcca aaagaggtgc aagggctctg ggaatcgaat ataatcccga catggtcaac
     5461 ctttcaaaaa gaaatgcagc aagggcgggt gtcagcggga aagcagaatt cattgaagcc
     5521 gacctgtacg acaccgattt gtcaaaggcc actgtaataa ccatgttcct ccttccggaa
     5581 atcaacctca agttaaggcc gagactactg gaactcaaac cgggcacaag gattgtctca
     5641 aacaccttca ctatgggaga atgggaagct gacgtacacg tcacaaccga agacaactgg
     5701 aacagctgga acactgctct tatgtggatt gttccggcaa aagctgaagg cacatggaaa
     5761 ctgggcaggg atgaacttat cctgacccag gaatatcaaa tgctgcaggg caggcttaag
     5821 cagggtgaaa aaactgtctc aatcagcggc ggcagactga aaggaaatga aataacattc
     5881 agggcaggtg attatttata tacaggcttt atcaacggca acaggatgac cggcaaggta
     5941 acggattctg ttctgggcaa taccggtgac tggactgcag ttcgttaagc cctgctcttc
     6001 tgaaccgctt cacaagtcgt ttttcagact gccccggatc tggcaaggcc gggatatcag
     6061 ttactgcttc ggacgtccct tcaacagctt ctgaagtccg ttcatctcgt ccctgaatct
     6121 tgcagcctca ataaaatcaa gctccgaagc tgccttttcc atttttttcc ttgtggcctc
     6181 aatggctttc tctagagcct cacggttcat gtactttatt acgggatctg ctgcaatatc
     6241 aacagggccg ggttcaatat aggcttttac cgtaacaccc tttttactga gtccggccag
     6301 gatggttccg gtttttctga caatccgggc aggagttata cccatttctg cattgtattt
     6361 caactgcttt tcccgccttc ggtctgtttc attgatcgtc atctgcatac tgttggttac
     6421 agtgtcggca tacattataa ctttcccgtt caggttacgg gcagctctcc cggcagtctg
     6481 tgtaagtgat ctggcggatc tcagaaagcc ttccttgtca gcatcgagga tggctaccag
     6541 cgatacttcc ggcagatcca gtccttcacg cagaagattc acccccacaa gaacatcaaa
     6601 cgtgccagcc ctcagtcctt ccataatctc tatgcgttca attgtatcga tgtccgaatg
     6661 gatgtatctg cttttaatat cgaactttat gaaataggca ttgagctctt cggccattct
     6721 cttggttatg gttgtgacaa gagttctctc acctgccagg gcccgctctc ttatttcatc
     6781 cataaggtga tctatctgat tcagtgtcgg ccttacctcc acgggaggat ccagcaggcc
     6841 cgtaggtctc agaacctggt cgacgaacac tccctcgctt ttttcaagtt cataatctgc
     6901 aggagttgca ctcacataaa tcacctgacc tgtaagagac tcaaactctt ctatacgcag
     6961 gggcctgtta tccagggccg caggcaatct gaagccgtat tcaaccagtg tctgtttccg
     7021 tgaatgatct ccgccgtaca tgcccctgat ctggggaatg ctcacatggc tttcatcaat
     7081 gaccgttatg aaatcccgcg gaaaataatc aagaaggcag aatggtctgg tgccggccgg
     7141 acggccgtca aaatagcggg aataattctc tataccgctg caataaccca gttccttaat
     7201 catttcaaga tcatacgaca ccctctgctc aagccttttt gcctcttcct tcttccctat
     7261 gtcattgaaa tatatcacct gcttcaccag gtcatcctgt atcatgccta ctgcctgatt
     7321 tatcctgtca cgggtcgtga caaatatatt ggccggataa atgatatagt catcgagctt
     7381 ttcaaaaaca tcaccgcttt ggggatcaaa tgtatagatt tcttcaacct ggtcaccaaa
     7441 gaaaatcacc ctgacagcta tgtcggcata ggcaggaaaa atatcaaccg tatcccctct
     7501 aaccctgaat gtgccgtgcc tgaattcaag ctcattccgt gaatacaggc tgtcaaccag
     7561 tttcctgaga aactggttcc gcccggtttt tttcccggtt tcaaggtgta cggtattgga
     7621 atgataatca tccggattgc cgatcccgta aagacaggaa acggaagaga ccacaagcac
     7681 atcctttctg cccgacagga gtgcggatgt tgtactgagc ctcagttttt caatatcttc
     7741 gtttatcgac aggtcctttt caatataggt atcagtaaca ggcagatatg cttcgggctg
     7801 ataataatca tagtatgaaa cgaaatattc aacacggttc tccgggaaaa actgtctgaa
     7861 ttcgccatag agctgggctg caagggtttt attatgacta agtaccagaa ctggccgctg
     7921 tgccttttct ataacgtttg ctattgtaaa agtcttgccg gaaccggtta cccccagcag
     7981 agtctgatac ggaacaccct tttccagtcc ttcggcaagc tgtctgattg cctccggctg
     8041 atcgcccgtg ggaacaaaat ccgatatcag tttgaatttc attatttccg cttaactgta
     8101 tgttcttgtt ttaaaaagta aagttagaaa ttatctcttt gcctggtgaa gtctgaaagg
     8161 cttccgcctg acctgttccg tcagtgtttt ccggccatca ataagaaaag gcgctcccag
     8221 gcgcctcttc tttccggaaa tttccggtat actaacctaa ataacccttc agaagtttac
     8281 tccgcgatgt gtgcctgagc ctgcggatgg ccttttcctt tatctgcctt accctttcgc
     8341 gggtcaggcc gaatttctcc cctatttcct caagcgtcat ctcctggcat cctatgccga
     8401 aaaagtatct gattatatcc ctttcccgtt cagtaagtgt ggcaagtgca cggtatattt
     8461 ccctggataa tgattcatcc atcagcagtt tgtcggcaac aggtgaatca atattcgtaa
     8521 ggacgtcaag aaggctgttg tcttcaccct caatgaaagg ggcatcaaca gaaatatgcc
     8581 tgcctgaaac cttcagtgta tccgtcacct tttccttggg aagttcaaga acttctgcaa
     8641 gttcttcagc ggtcggagca cgttcattct cctgttcaaa cctcgagaat gccttattga
     8701 tcttgttcaa cgaccctacc tggttgaggg gcagccttac tattctggac tgttctgcaa
     8761 gtgcctgcag tattgactgc ctgatccacc acacagcata ggatataaac ttgaaacccc
     8821 tcgtttcatc aaatttttcc gcagccttta tcagaccgag attgccttca ttgataaggt
     8881 caggaagact cagaccctga ttctggtact gtttggcaac ggaaactacg aaacggagat
     8941 ttgccttggt gagtttttcg agagcatacc tgtctccctt ttttattcgc tgagccagtt
     9001 caacctcttc ttctaccgat atcaactcct ctttaccaat ttcctgcaag tacttgtcaa
     9061 gtgatgcact ctctctgttc gttatggact tggtaatttt aagctgtctc atgctttttg
     9121 aaaaattgtc gcaaagttaa tattatacta tacaataaca aaaattttta acagttgttg
     9181 ttaacagaaa aatgttaata attcaaatcc ctgttactct gttgtttata atattttcca
     9241 tattcgtttt cagattcagg aaaaatatca ggatccggat acagggcggc tgttaactct
     9301 gcagccgccg gacgtgccgg atccgaaaca gcacaggaac ctgaactcat tgaacaggtt
     9361 cctgaatcat ggtcatgtct cagtttccgt actggaagtg gaattccctt ccttccgggg
     9421 taataccggc aattgattta agtgatctct cgcttgttcc tgccgccgag cgaacatcat
     9481 cagaactgtt taccttttta ttgttgacag ctgtaattat tgtaccccgt cttattccca
     9541 gatccctgaa aagtccgtca cctatctcaa caattttaac gccgctgtta atgccgtacg
     9601 atcttttatc agcctgactc actggctcaa atcttgctcc gaacaccgca ccgctacctg
     9661 aaccgcgggg aaccacatcg gttgttccgg aaatattctt aagagtcagc gaagctgtgt
     9721 tttcctttcc gtttctgata aatgtgatac ttaccctgtc acccggacgg tgctttccga
     9781 cctgttcctg aagctcggtg gcattattca ccacgacacc gtcaaacctt atgatcacat
     9841 catccttttt gagattggca tctgccgcgg aacctccctc acgcacttct gtaatcattg
     9901 cacccttaac ctcgggcagt tttttctgtt ccgcaatttc ggaagtcaca tcggttatat
     9961 taacgcctat cagtgcgcgc tgaacctctc cgtactgtct tagatcatca accacctttt
    10021 gcacgatact taccggaatg gcaaaggaat tacctgcata tgcaccgctg ggggaataga
    10081 tagcagaggt tattcccaca agcaatcccc tggtgtcaac gagtgctccg ccgctgttac
    10141 ccatgttcag tgcggcatcg gtctggataa aggattcaat cctgtaatca ttgtcaagaa
    10201 tgcccaggtt ccgtcccttg gcacttacaa taccggcggt gacggttgag gtaagattga
    10261 agggattgcc cactgccaga acccactctc ccaccttcag cttatccgaa tctccgtatt
    10321 tcaggaagga aaggttttca gcctttattt tcaggagggc tatatccgtg ctcggatcac
    10381 ggcctatcag ttctgcactg aaaaccctgt tgtcattcag ttttacttca atattttcgg
    10441 catcctcaat aacatggtta tttgtaatta tatatccgtc tgaggatatt atcactcctg
    10501 atccgtagcc tgcaacttcc ctgggcctgc tccggtaacg atcgccgtag aaccattcca
    10561 tgatcggatt gctcggctgc tgaaccatgg attttgtccg gacatggacc actgcatgta
    10621 ctgtctgctc tgctgcatag gtaaaatcaa cctgcccggg aaggtcctga agagccgtaa
    10681 gataagctct cgcctcggaa gcctcggtcc gggaactgtc ttttgcaact accacggccg
    10741 gtttgtcagc aattctggta taagcaaaca gtgctattgc agctcccagg acagccataa
    10801 gtagtgcacc caataaattt ctgcctttca tatctttaaa gttttaaatt ttcctctggt
    10861 tattctccgt tcttttttca gattttatgc ctgataaaag attgatatct ttataaccat
    10921 tttctgtgaa tattgtttta caaagatcgt gcattttttc tatgagcttc aatattttag
    10981 aatgaattaa caacacttta acaatgatga cagtcacttt taacaaatac cagggagcgg
    11041 gtaacgattt cgttatcata gataatcgga agaatataat taaccctgac aattcagttc
    11101 ttattaacag attatgcgac cggagattcg gcataggggc tgacggattg attctcattt
    11161 cagaaattaa aggacatgat tacgaaatga aatatttcaa ttccgacggt aaacttggct
    11221 caatgtgcgg aaacggagga agatgcgcag cccattttgc catgaaacac ggaatagccg
    11281 gaaaagagca ggaatttatg gccttcgacg gcctccatca ggcaaaggtg gacaatgaca
    11341 ccgtcaggct tcagatggct gatgtaagtg accacaaatt agtggatgac aattatttta
    11401 tcgataccgg gtcgccacat tatatccttt tcactgatga cgttgacagt ctgaatgtat
    11461 acgaggaagg gaaaaggata agatggtctg aaaaattcgc accaggcgga accaatgtaa
    11521 attttgtcca ggttattgac aatggactta aaataagaac attcgaaaga ggtgttgaag
    11581 atgaaaccct ggcatgcggc acaggcgtga cagcatctgc cattgcttca gttctgaaag
    11641 gacattttgg cacatccccg gttgatgtca gggcacgcgg cggcaacctc agagtagaat
    11701 ttgaaataga caataagatg atcagaaaca tctggctgac cggacctgcc acttttgtat
    11761 acgaaggtaa attagatatc tgatcacaat tgccattgga aatataaatc cctcttctta
    11821 caaagaggag taaaatgaat gaacatatgc ctgaaaacac atcgccgtaa tccgttattc
    11881 ataaaccaat taaatataat tcagccatga aagcttacac cccgattatc ctttcagtat
    11941 tccttctcct gccctgcaaa ctgaattcac agccggggac aacagagttg atgaaagcca
    12001 ttgaggaaat ctcagcggac atcatcaggg aaatgatttc acagggctat gttgttgtag
    12061 ctgccgaata ccgaaaaaga tctccggcat ggcaggtgaa gaaataccgt ggaactccgc
    12121 tgctgattca cactaataca aacgatgagg atgttaatgt gctcgaagtg gaacacctga
    12181 taaaatcttt cagggcagaa gggaaaagcg gtttttcata tgaaatattt gaaaatatgc
    12241 caggcggaca ctcctttgac agaatggatt ataaagaagc caaagagata agaatgaaga
    12301 tttacaatca tctgaacaga tatcttaaac cacccaatcc cttcagatca gtaaaggatc
    12361 ttcagaaagc agggtacgga tttaattaac aggacattga aaaccagaat cagaaattca
    12421 caattgtgag taaggagaag ctttacggta aaacgctgga cgaacttgtt gctgtaacaa
    12481 aaaaggctgg tctgccgggc tattccgcaa aacagatcgc cggctggctg tacgggaaag
    12541 agattaatac gcttgatgag atgacaaacc tctccaaaaa gatgagggaa agattgtctg
    12601 aggagtatga gatcggtctt tcccctccgg caggtgttac ggaaagcagg gacggaacaa
    12661 aaaaatacct tttcaaagtg ctctccggca aatacatcga gtcagcctat attcccgaaa
    12721 agaacagggc taccctgtgt atctcctcgc aggccggatg caggatggcc tgcatttttt
    12781 gcatgaccgg cagacaactc ttccagggga atctctcatc gaacgagata ctcaaccagt
    12841 tccgcagtat tccggagttc ggcatgatca cgaatattgt gtacatggga atgggggaac
    12901 ctctcgacaa tattggggag gtacttaaaa gtctggagat c
//
LOCUS       DAOSPX010000057        12939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4843_length_12939_cov_4.549984, whole genome shotgun sequence.
ACCESSION   DAOSPX010000057 DAOSPX010000000
VERSION     DAOSPX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12939
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4843_length_12939_cov_4.549984"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1989)
                     /locus_tag="PLZ75_04500"
     CDS             complement(<1..1989)
                     /locus_tag="PLZ75_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOS71692.1"
                     /translation="MIYPDNFEQKIGFDRIRELLREKCLSSMGIEKVDSTGFDTDPDR
                     IREKLSATYEFQQLLVYEENFPSGNYYSFAATLEKIRIEGTFPEIREIFDLRRSLETV
                     RSILSFFRTREESRYPALKRLCRPVKTYPYVTESIDRIIDRHGSVKDNASPRLREIRS
                     EILSKNALVSRRLSAVLKQAQAEGIVDPDTAVTVRNGRGVIPVSVSGKKRLKGLIHDQ
                     SASGKTIYIEPQEIVEINNDIVELEYEEKREIVKILTRFADDIRPYIDDLLESSVFLG
                     EIDFLRAKAVFGNQFNSVKPVISDNPGISWKGAIHPLLFLAFKRLPDERKVIPLDIEL
                     DDRNRILLISGPNAGGKSVCLKTVGLLQYMLQCGITIPVRENSECGIFRDILIDIGDE
                     QSIENDLSTYSSHLINMKFFLRHGSERSLILIDEFGTGTEPMLGGSIAEAVLGELNRK
                     RVYGVLTTHYTNLKHFASVTEGIVNGAMAFDSHLMQPLFQLYTGKPGSSFAFEIARRI
                     GLPEEILNEASGKAGIENINYDRHLKDIARDKRYWETKRRNIRQHEKRLEEVIDEYER
                     ELAAAKSLGKEIMTRARDEAEKLLKEANRMIENTIRQIKESQAEKEKTRDVRKVLEDF
                     RTRISDEAFLPETDREKKISGLARKAKRLKPGKGSRETP"
     gene            2147..3721
                     /locus_tag="PLZ75_04505"
     CDS             2147..3721
                     /locus_tag="PLZ75_04505"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOS71693.1"
                     /translation="MKNFKACISLALMLMMPLWLLSQTEKVDLGMIYRIKQEGSNNSK
                     MEELAFGLTDLTGSRLTGSTGGDKGNEWAKKKMEEFGFQNVRIEEAGDFSRGGWDNLK
                     AYAAMTAPYYVNFACLPVAWTGSTNGLVRAEVILLDIKTESDLDKYRGKLNGKIILMP
                     SASAMPHEISFEPLASRYSEKQLEELTLENPPSSGGRAAQPFDMAAMMAQRQLRAKIT
                     EMLKNEGVSVILNNSGTYNIPRASGAFYSAGESEPVAEMNIPMEAHGRMERLIRNNVK
                     VEMEVEILNKFFPSPKIYNVIGEIPGTDKVLKNEVVLLGAHIDSWHGGTGAADNASGC
                     IVMMEALRILKSLNVAPRRTIRVALWGAEEQGLNGSRGYVEKYLYNPDTETNRPDYDR
                     FSVYFNMDNGTGRYRGIYLQQNEMARPIFEELLKPFESLQGNTITIRNTGGTDHLSFD
                     RIGLPGFQFIQDPIEYGRGYHSSMDTYERLIMSDLRINAIITASLAYHAAMRDEKFPR
                     KPVMTFPGRSPFGFPR"
     gene            complement(3905..6046)
                     /locus_tag="PLZ75_04510"
     CDS             complement(3905..6046)
                     /locus_tag="PLZ75_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOS71694.1"
                     /translation="MGTDKHIATIALKLGLHEWQVENTIRLLGDGATIPFISRYRKEM
                     TGSLDELKLLHIRDEYRRLQELDARRESIVRSIEEQEKMTPELMEKIKNAVTMAELED
                     IYLPFRPKRRTRATVAREKGLETLAELLFRQQETNPSLKAEEFLSDEVSTTEEALSGA
                     SDIIAEWINEDETARKRLRRLYEKEAVIYSKAVKGRETEGIKYSDYYDWSEPLRKCPS
                     HRLLAMRRGEEEGFLKISIEPGEENALEMLSSLFVKGRNESSKLVENAVKDSWKRLLS
                     PSMETEFRNISKEKADDEAIKVFSENLRQLLLAPPLGEKNVLAIDPGFRTGCKLVCLD
                     RQGNLLHNETIYPHPPHNQTALSVKKILSLVNAYKTEAIAIGNGTASRETEDFIRFIR
                     FEKDIQVFVVNEAGASVYSASKVAREEFPGYDVTVRGAVSIGRRLMDPLAELVKIDPK
                     SIGVGQYQHDVDQNKLQDALDEVVISCVNAVGVEVNTASSHLLAYVSGLGPQLAQNII
                     DYRTENGPFRSRQEFLKVKRMGTRTFEQSAGFLRIRNAVNPLDGSAVHPESYRVVEKM
                     AGDLGCDINELMKNEARRKEIRLDDYVTAQTGLPTLRDIMDELARPGRDPRSRIKEFR
                     FADIHEIEDLTPGMIVPGIVTNITKFGAFVDIGIQQDGLVHISNMSTSYIKDPSEVVK
                     IHQHVMVKVIAVDAERKRIQLSMKDTTGKTQ"
     gene            complement(6233..7414)
                     /locus_tag="PLZ75_04515"
     CDS             complement(6233..7414)
                     /locus_tag="PLZ75_04515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOS71695.1"
                     /translation="MERARIILKSGKDQSVKRFHPWIFSGAIKKIYGSPSEGDVVDVF
                     DNKNTFLARGHYQPGSIAVRILTFENTETGIDFFRERIRNAINYRESAVLKNTRETNA
                     FRLVHAEGDNLPGLIVDYYNGVVVMQMHSVGMYRLREELTEILKDLLGEKVRAVYDKS
                     GETVPYLSGIGARNGFLYGSSGPVEITENGFRFMVDIESGQKTGFFLDQRENRKMLAE
                     YSEGRNVLNLFAYTGGFSVYAMKNARMVRSVDSSASATGLADENIRLNFGDDKRHEAV
                     KADAFSYLAGTKDEFDLIILDPPAFAKHNNVLANALQGYKRLNLKAIEQIRPGGIIFT
                     FSCSQVVSRENFRKSVFAAAANTGRKIRILHQPGQPPDHPVNIYHPESEYLKGLVLYV
                     E"
     gene            complement(7534..8025)
                     /locus_tag="PLZ75_04520"
     CDS             complement(7534..8025)
                     /locus_tag="PLZ75_04520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOS71696.1"
                     /translation="MISQNSGKYFRNWPAIRFWVLIPRHAPLSARDERTRYLSFSCQP
                     AACLFRINRIGIPDELADILSDPSVIKAGVAIHDDLRFLTGIRKFTPDGFIDLQKFVK
                     EFGIKSSGLRKLAAIILGFRISKAQQITDWEAGELSEAQIVYAATDAWVCYEIYSKLM
                     NGR"
     gene            complement(8106..8723)
                     /locus_tag="PLZ75_04525"
     CDS             complement(8106..8723)
                     /locus_tag="PLZ75_04525"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOS71697.1"
                     /translation="MEKKTDPVYSRNVLEFVAAANEFCKYAERSAEIKGGELLRILQR
                     LLPYLYIRASFLPSLEPVFEDGNEKFVTESDWNTIHDSLKKQFGSTDEYLDIYDAGPE
                     GPDNIVQSSLSENLADMYQDIKDFLLLYQTGTEEIMNDAVWECKISYENIWGQKLVNS
                     LRAIHMFIYSGDEPEKDIKNENDGERDTSEWFISKRQREYGKNDE"
     gene            8960..9769
                     /gene="zupT"
                     /locus_tag="PLZ75_04530"
     CDS             8960..9769
                     /gene="zupT"
                     /locus_tag="PLZ75_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOS71698.1"
                     /translation="MNTENFLFALGLTVFAGLSTGIGSLMAFFSKKFSPKFLALSLGF
                     SAGVMIYISLIEIFPAARETLSLSFGQKAGYWYTVIAFFTGIGLIALIDRLIPSYENP
                     HEIHKLNGNSDKSEYQNHKLYRLGLFSATAIAIHNFPEGLATFLAALNDQTMGISIAV
                     AVAIHNIPEGVAVSVPIYFATRNKSKAFWLSFTSGLAEPVGALAGYFILKTFLGEGAF
                     GFIFAAVAGIMIYISLDELLPTAEEYGEHHLAIGGLVGGMMVMAVSLLLFA"
     gene            9989..12490
                     /locus_tag="PLZ75_04535"
     CDS             9989..12490
                     /locus_tag="PLZ75_04535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOS71699.1"
                     /translation="MDLNKIRLRYKNVSYLRKPEDLTLEEWQYALRRQYAWTHHFAVK
                     KQGNHPVFSDYEVRNPETGLRYKVAIRSKDNSANFCECGDFKTNGLGTCKHIEAVFRY
                     IDTVLNKSHLLNKEYNPVYSSIFLDYRGERKIRIRTGTENSKELQILAEKYFDENKVL
                     PESSYDIIDQIIQEAKTISESFRCYPDALSYIIEKREDHKRSKMVFEKYRLQISNGVF
                     KSLVNTELFPYQKEGICFAAEKGRCIIADDMGLGKTVQAIAATELLRKEAGITYALII
                     CPTSLKYQWKSEIEKFTGQQARVIEGLVDKRIVQYKNDSFYKIVSYNTAINDIEEIRQ
                     TLPDIIILDEAQRIKNFKTKISRNIKKLQAPYSFVLTGTPLENKLEELYSIVQFTNPY
                     TLGPFYRFLHEHRIADHKGKVIGYQNLNRIGEILSDTMIRRRKKDVLLQLPERMDKIL
                     YVPMTGAQSRIHEEYRQYVATLIAKWHRQGFLSEKDRQNLMIFLNTMRMVCDSTYILD
                     QTSRNDTKIDELLEILEKVFAEGDEKIVIFSQWERMTRIVSQELDARNIKFQYLHGGI
                     PGSARGKLFENFNNDPDCRVFLSTDAGGVGLNLQAASLLINLDIPWNPAVIEQRIARI
                     YRIGQKRNVSVINMVSTGTIEHKMLSVLSFKKGLAEGILDQGDNTIFLNNSKFKVLMN
                     SVEEMISSEPAITDQPEEIEASTADQVAGENVDEIFIEESTATGDVTRNATGDFTGIP
                     AFLGDDDIVQKEAENKKVADKPVAPEDIIQTGLAFLGKLGQTLASPEATKQFVSSIVQ
                     KDASDGKTYIKIPVESEQAIENVLTMFAGIFNRKE"
     gene            complement(12538..12744)
                     /locus_tag="PLZ75_04540"
     CDS             complement(12538..12744)
                     /locus_tag="PLZ75_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71700.1"
                     /translation="MYSDKWISVKSIIENQDVSEVEIKERSYAKQIIIFEEYNRKIEE
                     ARKRKEKETALDCKSIYRIPDRYR"
BASE COUNT         3565 a         2967 c         2688 g         3719 t
ORIGIN      
        1 aggagtttca cgggatcctt tcccgggttt cagccttttt gccttccggg caaggccgga
       61 aatcttcttt tccctgtcag tctccggaag aaatgcttca tcagaaatcc tggtcctgaa
      121 atcctcaagc accttccgca catccctggt tttttctttt tcagcctgcg attcctttat
      181 ctgccgtatg gtattttcaa tcatcctgtt ggcctctttc agcagctttt ccgcttcatc
      241 cctggccctt gtcattatct ctttcccgag gcttttagct gcggccagtt ctctttcata
      301 ttcatcaatg acttcttcca gacgcttttc atgctgcctg atattccggc gttttgtctc
      361 ccagtatctc ttgtcgcggg ctatatcctt cagatgcctg tcgtaattta tattctcaat
      421 ccccgccttg cccgaggcct cattcagtat ttcctcggga agaccgatcc gtctggcaat
      481 ctcaaaggca aaggagcttc ccggctttcc ggtataaagc tggaaaagag gctgcataag
      541 atgactgtca aaggccattg caccgttaac tattccttcc gtaactgaag caaaatgctt
      601 gagattcgta tagtgtgtcg tcagtacgcc gtaaaccctc ttgcggttaa gctcaccaag
      661 aactgcctct gcaatcgatc cgcccagcat gggctcggtt ccggtgccga attcatcaat
      721 gagtataagg cttctctcag aaccgtgccg caggaagaat ttcatgttga tgaggtggga
      781 actgtatgta ctgagatcat tttcaatact ctgttcatcc ccgatatcaa tgaggatatc
      841 cctgaagatc ccgcattccg aattctccct gacaggaatt gttataccgc actgcagcat
      901 atactgaagt agtcccacag tcttcaggca gaccgatttg ccgccggcat tggggccgga
      961 gataagcagt atccggttcc tgtcgtccag ttcaatatcc aggggaatga cttttctctc
     1021 gtcaggaagt cttttaaaag ccagaaaaag aaggggatga atggctcctt tccaggaaat
     1081 accgggatta tccgaaataa ccggcttaac cgaattgaac tgattcccaa aaacagcttt
     1141 tgccctcagg aaatctattt cgccaagaaa tacagacgat tccagcaggt catcaatata
     1201 gggcctgata tcatcggcaa aacgggtaag gatttttaca atttccctct tttcctcata
     1261 ttcaagttca acaatatcat tgtttatttc aactatttcc tggggctcta tatatattgt
     1321 cttgccggaa gccgactgat catgaataag gcctttcagg cgcttcttcc cggatacgct
     1381 gaccggaatc actcccctgc cgttcctgac ggttactgca gtgtcagggt cgacaattcc
     1441 ttcagcctgg gcctgtttaa gcactgcact tagcctcctt gaaacgaggg cattctttga
     1501 caggatctct gatcttatct ccctcaggcg cggggatgca ttatccttta ctgaaccatg
     1561 cctgtcaatt atcctgtcaa tggattcagt gacataggga tatgtcttta ccgggcggca
     1621 cagccttttt agagcaggat acctggactc ttcccttgtt cggaagaagc ttagtattga
     1681 tctgactgtt tcaagcgacc tcctcaggtc aaaaatctcc cttatttcag gaaacgttcc
     1741 ttcaatacgg attttttcaa gagtggctgc aaaactgtaa taattgccgg acggaaaatt
     1801 ttcctcataa accagaagct gttgaaactc atatgttgct gacagtttct ccctgatcct
     1861 gtccggatcg gtatcaaaac ccgtactgtc gactttctca atacccatgg aactgaggca
     1921 cttctccctc aggagctctc ttatgcggtc aaaacctatc ttctgctcaa aattatccgg
     1981 gtaaatcatc tgtcaaataa aatacaaaat tataaaagaa acatcacaaa tgctgaatct
     2041 caaacttgta gttaattgtt gttaaataaa tccggattaa agctaattaa cagctttttc
     2101 tttattttta acactgatta taacaaaacc aaaacagaat aataacatga aaaattttaa
     2161 agcctgtatc agtctggcac tgatgcttat gatgccgctc tggcttttgt ctcagacgga
     2221 aaaggtcgat ctcggtatga tctacaggat caagcaggaa ggcagcaata attcgaagat
     2281 ggaggaactt gctttcggac tcactgatct taccggatca aggctgaccg gctcaacggg
     2341 aggcgacaaa gggaacgagt gggcaaagaa aaagatggag gaatttggtt ttcagaatgt
     2401 gagaatcgag gaagcaggcg atttcagccg cggaggctgg gacaatctga aagcatatgc
     2461 tgcaatgaca gctccctact atgtaaattt tgcctgcctt ccggttgcat ggacaggaag
     2521 taccaacgga cttgtcaggg ctgaagtaat ccttctggat ataaaaacgg aaagtgatct
     2581 tgataaatac aggggtaagc tgaatggaaa aataatcctt atgccttcag catcagccat
     2641 gccccatgaa atcagctttg agccgctggc atcaagatat agcgaaaaac agcttgaaga
     2701 gcttaccctg gagaatcccc cgtcttcagg aggaagagcg gcccagcctt tcgacatggc
     2761 tgccatgatg gcacagagac agctcagggc gaagatcacc gaaatgctga aaaacgaagg
     2821 tgtaagtgta atcctcaaca attcaggaac atataatatc ccgagggctt caggggcttt
     2881 ttacagcgcg ggagaaagtg aaccggttgc agaaatgaat attccgatgg aagctcacgg
     2941 acgtatggaa aggctgatca ggaataatgt gaaagtggaa atggaagtgg agattttgaa
     3001 caaattcttt cccagtccga aaatatacaa tgttattggt gagataccgg gaacggataa
     3061 ggtcctgaaa aatgaagtgg tgctgctggg tgcacatatc gattcgtggc atggcggaac
     3121 gggcgcagcc gacaatgcgt caggctgtat tgtaatgatg gaggccctcc gcatactgaa
     3181 aagcctgaac gtggcaccgc gcaggaccat cagggtcgct ctctggggtg ccgaggaaca
     3241 ggggctcaac ggttcaagag gatatgttga aaaatatctg tataaccctg ataccgaaac
     3301 caacaggccg gattatgaca ggttttccgt atacttcaat atggataacg gtacaggccg
     3361 gtaccgggga atatatctcc agcagaatga aatggcaaga cccatatttg aagaattgct
     3421 caagccattc gaatcactgc agggcaatac aataacaatc agaaatacag gtggaaccga
     3481 tcatctttcc tttgaccgta tcggtcttcc gggatttcag ttcatccagg atcccattga
     3541 atacgggcgt ggctaccatt cctcaatgga tacctatgaa aggcttatca tgtcggatct
     3601 gaggataaat gccataataa cggcatcact ggcatatcat gctgcaatga gagatgaaaa
     3661 attcccaagg aagccggtaa tgacctttcc gggaagatca ccctttggat tcccccggtg
     3721 agaattttta acagagtctg atcccggaat tgccacgggg aaaaattacc tggaacggca
     3781 accttttagc ttccaggaag agaaaccttt attaaggttt ctccagcagt tatccggatg
     3841 atgacataat accattgcta aaccttagta ttaccctctg gatttccccg gcgccctgat
     3901 gttgttattg agtttttccg gtggtatcct tcattgaaag ctgtatcctt tttctttcag
     3961 catcaacggc aataaccttt accattacat gctgatgtat cttcaccact tccgaaggat
     4021 ctttaatata ggatgtgctc atatttgaaa tatgcaccag gccgtcctgc tgtattccga
     4081 tatccacgaa tgccccgaat ttcgtgatat tggtaacgat tcccggaaca atcattcccg
     4141 gagtaagatc ttctatttcg tggatatctg caaaacgaaa ctcctttatc cttgatctcg
     4201 ggtcgcgccc tggtctggca agttcatcca tgatgtccct gagtgttggc agtccggttt
     4261 gtgctgtaac ataatcatcc agccttatct ctttccgtct tgcttcattt ttcataagtt
     4321 catttatgtc gcagcccaga tcccctgcca ttttttcgac aacccggtag ctttcagggt
     4381 gaaccgcact gccgtcaagc gggtttacgg cattccggat acgaaggaaa cctgcactct
     4441 gttcaaaggt ccgggtcccc atccttttta ctttcaggaa ctcctgccgc gatctgaagg
     4501 gcccgttttc agtcctgtaa tcaataatgt tctgtgccag ctgcggaccg agtccggaca
     4561 cataggcaag aagatgactg ctagctgtat tgacttcaac gcccactgca ttcacacagc
     4621 ttataaccac ttcatcaaga gcatcctgca atttattctg atcaacatca tgctgatact
     4681 gccccactcc tatcgatttc ggatctatct tgaccagttc tgcaagcgga tccataagac
     4741 gccgtccaat ggaaacggct ccccggacag taacatcata accgggaaat tcctcccttg
     4801 caacctttga ggcggaatat actgatgcac cggcctcatt cacaacaaat acctggatat
     4861 ccttttcaaa cctgataaac ctgatgaaat cttccgtttc cctgcttgcc gttccgttgc
     4921 cgatggcaat tgcttccgtt ttgtaggcat ttaccagtga cagtatcttt ttcacggaaa
     4981 gggcggtctg gttatgcgga ggatgcggat atattgtttc attatggagg aggtttccct
     5041 ggcggtcaag gcataccagc ttgcatcctg tcctgaatcc cggatcaatg gccagtacat
     5101 tcttttcgcc gaggggtggt gccagaagca gctgacgaag attctccgaa aagactttta
     5161 tggcctcatc atccgccttt tccttggata tattcctgaa ttcagtctcc attgacgggg
     5221 aaagaagccg tttccagctg tctttgacgg cattttcaac aagttttgat gattcatttc
     5281 gcccttttac aaaaagagaa ctcagcatct caagggcatt ttcttccccc ggttcaattg
     5341 atatcttcag aaaaccttcc tcttctcccc ttctcatggc aagcagccga tgggacggac
     5401 atttcctgag aggctccgac cagtcataat aatcactgta cttaatacct tccgtctccc
     5461 tgccttttac tgcctttgag taaataaccg cctccttttc ataaagacgc ctcagtcttt
     5521 tccttgctgt ttcatcttca tttatccatt cggctattat atcggaggct ccggaaagtg
     5581 cctcctccgt ggtgcttact tcatcactca ggaactcttc ggctttcaaa gagggattgg
     5641 tttcctgctg cctgaaaagc aactcagcaa gtgtttcaag acctttctcc ctggccaccg
     5701 tcgccctggt ccttcttttt ggtctgaaag gcaggtagat atcctccagt tcggccatgg
     5761 tcactgcatt cttaatcttc tccatcagtt caggagtcat tttctcctgc tcttctatgg
     5821 atctgacaat actttccctt cgtgcatcca gttcctgaag cctccggtat tcgtcacgga
     5881 tatggagcag tttcagctca tccagactgc ctgtcatttc cttgcggtac ctgcttataa
     5941 aggggattgt tgccccgtcg ccaagaagac ggattgtatt ctcaacctgc cactcatgaa
     6001 gaccgagttt taacgcaatt gtcgcaatgt gtttatctgt acccatattt tataaaccaa
     6061 ttcatttatc aaatcaaaac cttcaccgcc tgcaattcac catatataac tgtattcccg
     6121 gattttcgcc gtttattcaa agtatacttc ccgtccctga agatacaggc caaccctgaa
     6181 gataccggcc gggaaatcaa tatatacatc aggaacatca gtttctcatt gttcattcca
     6241 catataatac cagtcccttc agatactcgc tttccggatg ataaatattc accggatgat
     6301 ccggcggctg cccgggctgg tgaagaatcc ttatcttccg gcccgtattt gcggcagccg
     6361 caaacactga tttcctgaaa ttctcccttg agacaacctg ggaacaggaa aaggtaaata
     6421 ttattcctcc aggcctgatc tgttctatag ctttaaggtt taatctttta tatccctgaa
     6481 gggcatttgc aagcacattg ttgtgttttg caaacgccgg cggatcaagt attatgagat
     6541 caaattcgtc cttagtaccg gcaaggtacg agaatgcatc tgcctttaca gcctcatgtc
     6601 ttttgtcatc cccgaaatta agccgtatat tttcatccgc cagcccggtt gctgaagcgg
     6661 aactgtcaac ggaacggacc atccgggcat ttttcattgc atacacggaa aagcctcctg
     6721 tataggcaaa caggtttaga acatttcttc cctcagaata ttcagcaagc attttcctgt
     6781 tttccctctg gtcaaggaag aatccggttt tctgtcccga ttcaatatca accataaacc
     6841 gaaatccgtt ttcagtaatt tccacaggtc ctgaactgcc gtaaaggaat ccgtttcttg
     6901 ccccaatacc cgacaagtac ggaactgttt ccccgctttt atcatagaca gcccttacct
     6961 tttccccgag taaatccttc agtatttccg taagctcctc tctgagtctg tacatcccca
     7021 cggaatgcat ctgcataacc accaccccgt tgtaataatc aactatcagg ccgggaagat
     7081 tgtctccctc cgcatggaca agcctgaaag cattggtttc cctggtattt ttcagtactg
     7141 cagattccct gtaattaatt gcatttctga tcctttccct gaaaaaatca attccggttt
     7201 cagtgttttc gaaagtcagg atcctcacgg ctatggatcc gggctgataa tggcccctgg
     7261 caagaaatgt gttcttgttg tcaaaaacat ccacaacatc cccttccgac ggagatccgt
     7321 agatcttctt tatggctccc gaaaatatcc atgggtgaaa tctttttacg gattgatcct
     7381 tgcctgactt taatatgatt ctcgctcttt ccatcacctg gattatctgt taataagggg
     7441 agtcatgtct gataattttc aacaacaaaa tatataaact gtaagatctg gtatctgtgt
     7501 aacagaaaat tccggtcttc ctttctgaaa aatctatctg ccgttcatta gtttgctgta
     7561 gatttcatag cagacccagg catctgtggc cgcatatact atctgggctt ctgaaagctc
     7621 acctgcttcc cagtctgtaa tctgctgggc cttagatatt ctgaaaccaa gaatgattgc
     7681 cgcgagtttc ctgagtccgg agctttttat cccgaattcc ttcacgaact tctggagatc
     7741 aataaatcca tccggggtaa atttcctgat gcctgtcaga aaacgaagat catcatgaat
     7801 cgcaactcct gccttaatga ctgagggatc ggaaagtatg tcagcaagtt catccggtat
     7861 ccctatcctg tttattctga aaaggcaggc cgccggttga cagctgaatg agagatacct
     7921 tgttctttcg tcccttgcgg aaagaggggc gtgtctcggt atcaaaaccc agaaccttat
     7981 ggccggccag ttccggaaat attttcctga attttgtgat atcatccact accactatcc
     8041 tccctctgaa ccaggcaaga tcataatttt tgatttcttc agcagtaata ttttcaggat
     8101 aagtgctatt catcattttt tccgtattcc ctctgacgtt tagagataaa ccattcagaa
     8161 gtatctcttt ccccgtcatt ttcattttta atatctttct caggttcatc tcccgagtaa
     8221 atgaacatgt gaattgccct caatgaattg acaagtttct gtccccagat attttcataa
     8281 cttattttac attcccagac tgcatcgttc atgatttcct cggttcccgt ctgatacaga
     8341 agcagaaaat ccttgatgtc ctggtacata tcagcaagat tttctgaaag actggactgt
     8401 acaatattat ccgggccttc cggtcccgca tcgtatatat caagatattc atcggtactg
     8461 ccaaactgtt ttttcagtga atcatgaatg gtattccagt ctgattcggt gacgaatttt
     8521 tcattcccgt cttcaaaaac cggttccagt gatggcagga aggatgccct gatataaagg
     8581 tagggaagca gacgctgaag gatcctgagg agctctcctc ccttaatctc cgcggaacgt
     8641 tcggcatatt tgcagaattc gtttgctgca gccacgaatt ccaggacatt ccgggaatat
     8701 acaggatcag tctttttttc catgtttttt atttgactgc aaaattagac aatttttgga
     8761 gagcgcttat aaaatcattt ttctgaaaaa tatcaggagg gttaaaacat aaaaatcaag
     8821 cagagaaaag tttttccgtt ccagggagaa gtaactattt cccggagaag ggaaaataca
     8881 gtttcccgca ggaggggaaa aaacagcatt aatatattat tatatttacc ctgttcattt
     8941 acgtaaaaga atcatatcga tgaacacaga aaacttttta ttcgccctgg gactcacggt
     9001 ttttgccggc ctttcaaccg gcattggcag tctgatggca tttttttcaa aaaaattcag
     9061 tccgaaattt ctggctcttt cccttggatt ttcagcaggt gtaatgatat atatttctct
     9121 tatagaaatt tttcctgcgg caagggaaac attaagtctg tccttcggac aaaaagcagg
     9181 atactggtac actgtcattg ccttttttac gggtatcgga cttatagcac tgatcgacag
     9241 actgatcccc tcctatgaaa atccacatga aatacataag ctcaatggga atagtgataa
     9301 gtccgaatat cagaatcaca aattataccg cctcggtctt ttttccgcaa ccgcaattgc
     9361 cattcacaat ttcccggaag gactggctac ttttctggct gccctgaatg accagacaat
     9421 gggtatcagc attgccgtag cagtggcaat acacaatatt ccggaaggtg ttgccgtgtc
     9481 tgttcctatt tattttgcca caagaaacaa aagcaaggcc ttctggcttt catttacttc
     9541 aggcctggca gaacctgtcg gagcactggc cggatatttc attctaaaga catttctcgg
     9601 ggaaggagcc ttcggtttca tatttgcggc agttgccgga ataatgattt atatctccct
     9661 ggacgagctt cttccgacag ccgaggaata cggtgaacat catctggcaa ttggcggcct
     9721 ggtcggcggc atgatggtta tggcagtaag cctgctactg tttgcctgag gaaaatgaaa
     9781 acaaacgata gtctggcctg ccaattcagc attcttcaac cttttatgaa ataaaaatca
     9841 cagttacatt ttctttcacc tctcggtata ttgtttagta aaataaaaag caggcagttg
     9901 tttatttcct gtttaaaact tttgtaaaag ttaagccggc atgccggcca ttaaaaatca
     9961 taacgggata cttttgaaca aaaaaaccat ggacttaaac aaaatcagac tccgttataa
    10021 aaatgtctcc tatctgagaa agcctgaaga tctgaccctt gaagaatggc aatatgcttt
    10081 acgcaggcaa tatgcatgga ctcatcattt tgcagtaaag aaacagggga accatccggt
    10141 attttcagat tatgaggtac gtaatcccga aaccggctta aggtataaag ttgcgataag
    10201 aagtaaggat aacagtgcaa atttctgtga atgtggcgat ttcaaaacaa atggtctggg
    10261 cacctgcaag catatcgaag ccgttttcag gtatattgac actgttctta ataaaagtca
    10321 tcttctgaac aaagaataca atccggttta tagttccata ttcctggatt acaggggtga
    10381 acggaaaatc agaataagaa caggaacaga aaacagtaag gaactgcaga ttctggctga
    10441 aaagtatttc gatgaaaata aagtacttcc cgaaagttca tatgatatca tagatcaaat
    10501 aattcaggaa gccaaaacta tcagtgaaag tttcagatgt tatcctgatg cgctttccta
    10561 catcattgag aagagggaag atcataaacg cagtaaaatg gtttttgaaa aatatcgtct
    10621 gcagataagt aacggtgttt tcaaatccct ggttaatacc gagctgtttc cctaccagaa
    10681 ggaaggtatt tgttttgccg ccgaaaaggg tcgttgtatt attgctgatg atatgggact
    10741 cggaaagact gttcaggcaa ttgcggcaac tgaattactc aggaaagaag caggtattac
    10801 atatgcactg atcatttgtc cgacatcgct gaaataccag tggaaatcgg aaattgaaaa
    10861 atttaccggt caacaggccc gggtgattga aggattggtc gataaaagaa tagtgcaata
    10921 taaaaatgat tctttctata aaattgtttc atacaacacg gcgataaatg atattgaaga
    10981 gatccggcag acattgccgg acataatcat actggatgaa gctcagcgaa ttaaaaactt
    11041 caagacaaag atctccagga atattaaaaa acttcaggca ccatattctt ttgtacttac
    11101 aggtacacca ctcgagaaca aattggagga attatattcg attgttcagt ttacgaatcc
    11161 atatacactg gggccctttt accgtttcct gcatgaacac cggatagctg atcataaagg
    11221 gaaagttatt ggttatcaga atttaaacag gataggggaa attttatccg acaccatgat
    11281 acgccgtcgt aaaaaagatg ttttactgca gctgccggaa agaatggata agatcctgta
    11341 tgttcccatg accggggctc aaagcagaat tcacgaggaa taccggcaat atgttgccac
    11401 acttattgct aaatggcaca ggcagggttt cctgagtgag aaagaccgtc agaatctgat
    11461 gatatttctt aacacaatgc gtatggtttg tgacagtact tatattcttg atcaaaccag
    11521 ccgtaatgat accaagattg atgaactgct tgagattctt gaaaaagtat ttgccgaagg
    11581 cgatgaaaag atagttattt tcagtcagtg ggaacggatg acccgtatcg tttcgcagga
    11641 acttgatgcc cggaatatta aatttcaata tctgcacggc ggtattccgg gatccgcccg
    11701 tggcaaactt tttgaaaact tcaataatga cccggattgc agggtgttcc tctcaacaga
    11761 tgcaggaggt gtgggactta atcttcaggc ggcctcactg cttatcaacc tggatatccc
    11821 atggaaccct gccgtaattg aacaacggat cgcccgaatc tacaggatcg gacagaaaag
    11881 aaatgtttct gtgataaata tggtatcaac cggtaccatt gagcataaga tgctgagtgt
    11941 acttagtttt aaaaaaggat tggcagaagg tattcttgac cagggtgata atacaatttt
    12001 tctgaataac agtaaattca aagtgttgat gaattcggtt gaggaaatga tctcctctga
    12061 gccggccata acagatcagc ccgaagagat tgaagcttca acagcagatc aggttgccgg
    12121 agaaaatgtt gatgaaatat ttatagaaga aagtaccgcc acaggagatg tcacaagaaa
    12181 cgccacagga gatttcacag gaattcctgc tttcctcggg gatgacgata ttgtgcagaa
    12241 agaagcagaa aacaaaaaag ttgcggacaa acccgtcgct cccgaagata ttattcagac
    12301 aggtctcgcc tttcttggta aactgggaca aacccttgcc agtcctgaag ccacaaaaca
    12361 atttgtttca tccatagtac aaaaagatgc atctgatggc aaaacataca ttaaaatacc
    12421 cgttgaaagt gaacaggcga ttgaaaatgt actcaccatg ttcgcaggaa tatttaaccg
    12481 taaggaatga tatttttctc aagccatgta aaaggatgat gcgatttttc acgctgctta
    12541 gcggtagcgg tctggtattc tataaattga cttacaatct aaagctgtct ctttctcttt
    12601 tcttttcctt gcctcttcaa ttttcctgtt atactcctcg aagattatta tctgttttgc
    12661 ataagatcgt tctttgattt ctacttccga tacatcctgg ttttcaataa tacttttaac
    12721 cgagatccat ttatcgctgt acattatttg caaatccgga ttaaggatgt agcttcccct
    12781 cgatattctt ttaaataata ttttatatga ttatccgtta acatgccaat atgattatat
    12841 ttgtgcaaaa atttttcaac atgaacttga ttgattttgc cagacagcat cctgatgagg
    12901 aaagttgtat acagcatttt cggcttgcca aagagaaga
//
LOCUS       DAOSPX010000058        12791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4924_length_12791_cov_4.479036, whole genome shotgun sequence.
ACCESSION   DAOSPX010000058 DAOSPX010000000
VERSION     DAOSPX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4924_length_12791_cov_4.479036"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1173)
                     /locus_tag="PLZ75_04545"
     CDS             complement(133..1173)
                     /locus_tag="PLZ75_04545"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOS71701.1"
                     /translation="MKKLLFVFIFSCVLFSCKTSQLTLSVTEPAPVTLPREIRKVGVI
                     NRSIPTDETKLLDIIDKALSLEGADLDRDGAEQCVKGLTEELMTNRRFDEVRNLAEID
                     FRTPKLGNIFPVPLSWEIVDKIAEEQEIDALFSLDYFDTEARVNYSTGRTDIKAPGGI
                     VVPAVEHRAEMETIVRTGWRIYCPADRLIADEYHDLQSVKYSGRGVSPLVAVAGLIKR
                     KDAIMEVSRLAGHDYALRLIPFSRRVSREYFVRGTDNFRIARRKAQAGQWEEAGMLWE
                     KETSGSRPKIAGRACHNMGIISEINGDVKLALSWAQKAYGDYNIKKSLNYVRILENRI
                     RKADLLEYQKIQ"
     gene            complement(1272..1979)
                     /locus_tag="PLZ75_04550"
     CDS             complement(1272..1979)
                     /locus_tag="PLZ75_04550"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS71702.1"
                     /translation="MPDNFKHIIMTSHTSAAGTVFWLIIPLLIICCSGGSGKPGPADE
                     NAGIPEYDSTFLFNGRDLDGWEITNFGPQGPVYVSGTTIVLGMGEGCTGITWKKEFPE
                     MNYRISLEARRVAGNDFFCGMTFPAGKEPCTLIVGGWGGTTVGLSTINSMDASENQTT
                     NFREFEKNRWYRISLLVHADTIRALIDDEVVVNFVKGKYDELSIRPEVELSKPFGIAS
                     WYTTAQLRNIRLDKLDD"
     gene            2085..3593
                     /locus_tag="PLZ75_04555"
     CDS             2085..3593
                     /locus_tag="PLZ75_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71703.1"
                     /translation="MTKSKKINRREFLGSAAAGSLALTVVPRSVLGGPGYVAPNDRLT
                     IGYIGCGTQGLREMVSLIANPALQIVAVSDPNKFTTDYIDWSLHGIRNGIRKALGDPG
                     WGENFRGIPGGRDIGQELVQKYYARSRDMSNYKGCASYEDYRELLEKERDIDAIKIMT
                     PDHLHAHVAIHSMNKGRHVVTHKPIANRMKEFRATYDTAKKTGLRTHLLAWSPKPGYD
                     LAKKWIREGRIGTLREIHNWSNRPVWPQWQSNPTEKVPVPEGFNWDLWLGPVPARPYH
                     PNYTHNVFRGWYDFGGGSIADMGHYSLFPLFLTFGIDKPALSADAYGTTTAMAENNVC
                     RGVVNNVAFPLSCMIRFEMPATRELGAFDLYWWDGGMRPFTPEELLSERKSFEREGMM
                     FVGDRGKILAGFRGENPRLIPESRMAGISDKEPESSEPDTNEAWIQAFRSNSEARGSF
                     LLAGPVSETILLGTVSLRAGKRVEYDSESMTITNAPEANQFLTREYRPGWEI"
     gene            3581..4579
                     /locus_tag="PLZ75_04560"
     CDS             3581..4579
                     /locus_tag="PLZ75_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS71704.1"
                     /translation="MGNIIKENLTTLLKIIAMTSISRRSFIEKSLLTAAAGVAAPTLL
                     EAAAGSQTSAASPASPVSPASPESPASLASPASPTPRYVKDDISLAQWALVDEIRAGK
                     WKTLDFPKVAKNDFGLNGIEFVNTLFEVPTEGYLRRLKRNAGDLGVTMVLIMVDDEGD
                     GCSVTSEERKQFVTNHRKWIDAAVYLGCHAIRTNCVGPKQIDRNEALKHAAETYNMLL
                     EYAVPLNISVLIENHGGLSNDADWMVSLMKTVNNLYFGSYPDWRQPSDNFDNVDYLVK
                     MLPWAGGMSYRNQPTEELTARMINLAKSSGYRGWYGIESSGREEIKKGITLLKKYL"
     gene            4590..6632
                     /locus_tag="PLZ75_04565"
     CDS             4590..6632
                     /locus_tag="PLZ75_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71705.1"
                     /translation="MTRKMLLLAITVITACSGPGGGKREAQPALFISEETIKTAIRDF
                     KPESPDADIALFEKGVRHAASLWRESDGTAAEFLSFVKDNYISDPLKRRTVFNKTSDY
                     FESLYGYFNEITLSLRKNLDEARGEIDGIDRMFGNYSPGAHLQDDLYSNKIAFVTALN
                     FPYFTLAEKEELGPSWSREQWAMARLGDIFVSRIPAELIQALSVATGNAEMYIAGYNI
                     HMGRLLTDEGRKIFPDDMVLLSHWNLRDEIKADYADRLNGREKQEMIFSVMERIIRQE
                     IPEVVINNPETDWAPFTNRVTKDGRPLESAAENDMRYQHILNTFRAMKAIDPYVPEMN
                     TAILRSFSWDMEISQEEVEALFDNYLRSPRLAKIAGVIRERLGRDLRPYDIWYDGFKS
                     RSSISEDFLSSRTAKLYPSPEAFRAGMPAMLKKLGWSPERAEYISSKIVVDPARGSGH
                     AWGAGRKGSLSRLRTRVPETGMDYKGYNIAVHEFGHNVEQTITLYDVDHYMMSGVPNT
                     AFTEATAFIFQQRDLMLLGISDSGPEKEKSEILDNAWSLMEIMGVGMVDMKMWKWLYE
                     NPGASAAMLREAVVRIATDTWNEYFAPVIGVKDTPLLAIYSHMVNAPLYLANYSYGHV
                     IQFQIEEFMKGKKLSDEIDRIYKQGRLTPQQWMTGAVGSKISAQPVLDAVDRALGN"
     gene            6647..8023
                     /locus_tag="PLZ75_04570"
     CDS             6647..8023
                     /locus_tag="PLZ75_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOS71706.1"
                     /translation="MGKEEINSILEEQLKFFRSGKTLDINFRLGILKKFRTLIIYHEK
                     EIADALWKDFHKPEFEVIATETGFVLKELNLAIKKLKKWARPKKVRTPFVHFPARSYI
                     THQPYGQVLVLSPWNYPFQLVFLPMLGAITAGNCVIVKASGKVPNITSVMQKIIGNFP
                     AGLVAMVNGDHSISEHLLSYKFDYIFFTGSTRIGKYVMQKASENLIPVSLELGGKNPC
                     VVTSDAKLGFAAKRIVWGKFINAGQTCICADYLLVDKKVKDRLLDLISAGLKEFYGEN
                     PEKSRDFARIINEENLLRLVALTKTGHIVAGGNSDTETLYLEPTVIKDITPGDLIMQE
                     EIFGPVLPVIEFENLSEVYNIIERNPKPLAVYIFTRSKKLAREFLARTSSGTAAVNDT
                     VMQIASPFLPYGGVGYSGMGRYHGRRSFETFSNTRSVLVKSNLIDLILRYPPYTKFSK
                     KIIRWLMN"
     gene            complement(8070..9032)
                     /locus_tag="PLZ75_04575"
     CDS             complement(8070..9032)
                     /locus_tag="PLZ75_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOS71707.1"
                     /translation="MIQEIGLKELDPDKFVEEQAESIKAAVGDGIAINALSGGVDSSV
                     VTMLGHRALGRQLRTVIVQNGLLREGETEWVSTVFHELGVEVEIIDARMEFLDSLKGL
                     TDPEEKREAVTNTFYKRVFGRLVRESGARFLLQGTILTDVDETVAGIKRQHNVFAQLG
                     IDPEEALGYQIIEPLVRLRKDGVRIAGRALGLPEEMLGRIPFPGPGLAARIIGEVTAE
                     RLETVRKATVIVEKALAGSGAFQYMAILHSDRVTGVREGRRDFGQQIEVRCWDSTDSR
                     TATPSRLPYEILEKMAADITDQVPGVVSVTYNITAKPPSTIEAI"
     gene            9842..11086
                     /locus_tag="PLZ75_04580"
     CDS             9842..11086
                     /locus_tag="PLZ75_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS71708.1"
                     /translation="MLLSLVISEKGIAQDNNWTHFRGSGLNGIASVETVPLNWNDDSG
                     FRWKTDIHGRGWSSPVVYGDQIWITTATDDGRELFAVCADFRNGKIIHDIKVFSVSDV
                     EGKHSLNSYATPTPCIEKGFVYVHFGSNGTACINTSNGNIVWTRTDLKCKHVQGPASS
                     PVLYKNLLILHYEGTDVRFITALNKSTGQTVWKTDRPQKPYEPLTVIGRKAYVTPLIL
                     NVKGKDLLITNGSAVCNAYNPDTGEEVWSLIGGAESTIAMPFSENGVLYYYTGFMRDP
                     SGTEYSELMAVNPDGKGDITASNIIWKKRSGRLQLLTPVIKDGLIYTVDSNNNLMCIE
                     AETGKEVWTTRVRANFNASPVLAAGRIYFFSVRGDVLVIRPGRKYEVLSQFQTGNPVW
                     ATPAFLRNSIILRTDKYIGRIE"
     gene            complement(11099..12055)
                     /locus_tag="PLZ75_04585"
     CDS             complement(11099..12055)
                     /locus_tag="PLZ75_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOS71709.1"
                     /translation="MKVLITGSSGSLARMLIGHLAEKNTEIVGIDIRECSDSLHGDKF
                     RFYKCSITEKEKLISIFAGEKPTNVVHFACSFNKVRNRKQEYEIDITGSDNVLKASQI
                     TPSVRQLIYISSAAAYGGKKNNPLWMSESEPLDPGKYRYGMNKKLVEHNYTNIKVRDD
                     LRIVILRPCQVLGPAFDKPKSIVSILIKVPFLPKFTRETKLQFIHSVDLVALMELILK
                     DNEISGIYNLVPDSYSTVKDIVPDKKFLPVPFFVIKGFLWICWHLKIMNIQPASIVHS
                     FYPTIVDSTKLTSRYGYKFKYSSKEAFEDTVVYNRIPEGSWL"
     gene            complement(12093..12245)
                     /locus_tag="PLZ75_04590"
     CDS             complement(12093..12245)
                     /locus_tag="PLZ75_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71710.1"
                     /translation="MAIKAFGNEASKLPFEVLETLAGDIINEVPGIVSVTYNISAKPP
                     SSIEAV"
     gene            complement(12272..>12791)
                     /locus_tag="PLZ75_04595"
     CDS             complement(12272..>12791)
                     /locus_tag="PLZ75_04595"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOS71711.1"
                     /translation="VFGCSIKWAWKNEYAEKLYEQWAKLPVTLEDIDVNGVMELIQNN
                     SSDKLRLINIWATWCGPCKTEFPELVIIDRMYCGRDFEFITISADKQDRKNDALKFLQ
                     SQQASNRNYIFSEDDVYRLIDAVGNGWRGALPFTLLAEPGGKIVYARQDMIDPHEMKK
                     LIVENKYIGRYY"
BASE COUNT         3536 a         3012 c         2879 g         3364 t
ORIGIN      
        1 tcagttcctg aaaatcagca gtctcctgcc ggataataag agaccgtttt ttcacagcct
       61 gcagctgatc ctgtaaatca tgtcataata ccggtcccgg cttcctgatc atcatggttt
      121 cctgaattaa agttactgaa ttttttgata ctccagcagg tctgcctttc gtatcctgtt
      181 ttccagtatc ctcacatagt tgagggattt ttttatgttg taatccccgt atgctttctg
      241 tgcccatgaa agtgccagct taacatcgcc gtttatctca ctgataatcc ccatattatg
      301 acaggctctt cctgctattt tcggtctgga accggaagtt tccttctccc agagcattcc
      361 ggcttcctcc cactggcctg cctgggcctt acgccttgct atcctgaaat tgtctgttcc
      421 cctgacaaaa tattcccggc tgacgcgcct gctgaaaggg ataagcctta aggcataatc
      481 atgtccggcc agtctgctga cctccataat tgcatccttc cgttttatca gtccggcaac
      541 ggccacaagc gggctgactc cccttcctga atacttcact gactgcaggt cgtgatactc
      601 atcggctatc agtctgtccg cagggcagta aatacgccat cctgtcctga cgatggtttc
      661 catttcggca cgatgttcaa ctgccggaac cacaatgccg ccgggagcct taatatctgt
      721 cctgcccgtt gaataattca ctctggcttc cgtatcaaaa taatcaagtg aaaacagggc
      781 atcaatttcc tgttcctctg caatcttatc cacaatttcc caggaaaggg gcactgggaa
      841 aatatttccc agtttcgggg ttctgaaatc aatttcagcc agatttctga cttcatcaaa
      901 cctgcggttc gtcatcagtt cttcagtaag tccctttacg cactgttcgg ctccgtccct
      961 gtcgagatca gcaccttcaa gtgaaagagc cttgtcgata atatcaagca gttttgtttc
     1021 atcagtgggg atgcttctgt taatcacgcc tactttcctg atttcccggg ggagtgtcac
     1081 aggagctggt tcggtaaccg aaagggtcag ctggctggtt ttgcaggaaa acagcacaca
     1141 gctgaaaata aatacaaata acaacttttt cataataatc atatgctatt ggttaaaaga
     1201 ctttcatttc acccgtcccc gccccggaac aattcaatgc agcaccgatg attccggttt
     1261 atgcgaaaat gtcagtcatc aagcttatca agcctgatat tcctcaattg ggcagtagtg
     1321 taccacgatg ctatcccgaa gggctttgac agttctactt caggcctgat ggacaattca
     1381 tcatatttcc ctttcacaaa attcacaaca acttcatcgt cgatcagtgc acgtatggta
     1441 tcagcatgca ccagcaggct gatccggtac catcggttct tttcaaattc cctgaaattt
     1501 gttgtctgat tctcagaagc atccatggag tttatagtac tcaggcccac agttgttccg
     1561 ccccagccgc caacaatgag ggtgcatggt tctttcccgg cagggaaggt cattccgcaa
     1621 aaaaagtcgt ttcctgcaac ccgcctggct tccagactta ttctgtaatt catttcagga
     1681 aattcttttt tccaggttat acccgtacat ccttcaccca ttcccagcac tatcgtagtt
     1741 ccggaaacgt atacaggtcc ctgggggccg aaatttgtta tctcccaccc gtccaggtcc
     1801 cggccgttga aaagaaaggt gctgtcatat tccggtattc ctgcattttc gtcagccggc
     1861 cccggctttc ccgaacctcc gctgcagcaa atgatcagta acgggattat cagccagaat
     1921 actgttccgg ctgcactggt atgacttgtc attatgatat gtttaaaatt gtccggcatg
     1981 catgtcctga ctaaatttca aatttaaaag attttagtga acctggtgta aaaaacgcct
     2041 gttttattaa cttcgtaact aagaaacctg taacccatac aaacatgaca aaatcaaaaa
     2101 aaatcaatcg ccgtgaattc cttggttcgg ctgctgccgg atcgcttgcg ctgacagttg
     2161 ttccaaggag tgtgcttggc gggcccggat atgtggcacc gaatgacagg ctcaccattg
     2221 gctacatagg ctgcggaaca cagggcctga gggaaatggt cagcctgata gccaatcctg
     2281 ccctgcagat agtcgcagtt tctgatccga ataaattcac tactgactac atcgactggt
     2341 cgcttcacgg aatccgcaac ggaataagga aagccctggg agatccgggc tggggcgaaa
     2401 acttcagggg aatccccggg ggaagggata tcggacagga acttgtacag aaatactatg
     2461 ccagatcccg ggatatgtca aattacaagg gatgtgcttc atatgaagat tatcgcgaac
     2521 tgctcgaaaa ggaaagggat atagatgcca tcaagatcat gacccccgat catcttcacg
     2581 cccatgttgc catacattcg atgaacaaag gcaggcacgt tgtcacccac aagccaattg
     2641 caaaccggat gaaggaattc agggcaacat atgatacggc aaaaaagacc ggattgagaa
     2701 cccatctcct tgcctggagt ccgaagcccg gatatgacct ggcgaaaaag tggataaggg
     2761 aaggccggat aggaaccctg agggagatac acaactggtc aaaccgtccg gtatggccgc
     2821 agtggcagag taatcccaca gaaaaggttc ccgttcccga aggctttaac tgggatctgt
     2881 ggctcggacc ggtaccagcc cgtccctacc atccaaacta cactcataat gtgttcagag
     2941 gatggtatga ttttggcggc ggcagcattg ccgacatggg acactacagc ctttttccgc
     3001 tcttcctcac attcggaata gacaaaccgg cactgagcgc cgatgcctat ggaacaacaa
     3061 cagccatggc agaaaacaat gtatgcaggg gagttgttaa caatgtggcc ttccccctgt
     3121 catgcatgat tcgctttgag atgcccgcaa ccagggaact gggagctttc gatctatact
     3181 ggtgggatgg ggggatgagg cccttcaccc ccgaagaact cctttctgag agaaaatcat
     3241 tcgaaaggga aggaatgatg tttgtaggtg acaggggaaa gatcctggcc ggtttcagag
     3301 gtgagaatcc aagactgata cccgaaagca gaatggccgg aataagcgat aaggaaccgg
     3361 agtcttccga acctgataca aatgaagcgt ggatacaggc attcagaagc aacagcgaag
     3421 ccagaggcag cttccttctg gcaggaccgg tttcggaaac cattctcctg ggaaccgttt
     3481 cgctccgtgc aggaaaaaga gtcgagtatg acagtgaaag catgaccata accaatgccc
     3541 ctgaagccaa tcagttcctc accagggaat accgtcccgg atgggaaata taataaaaga
     3601 aaacctgacc acccttttaa aaatcatagc catgacatcc atatcaagaa gaagttttat
     3661 agaaaaatcc cttttaactg ctgcggctgg tgtggcagca cccaccctcc tggaggcagc
     3721 agcgggatca cagacatccg cagcatcccc tgcatcaccg gtctcaccgg catcgccgga
     3781 atccccggca tcgctggcct cgccggcatc gcctacaccc cggtacgtaa aggatgacat
     3841 ctcccttgcc cagtgggcac tggttgatga aataagagca ggtaaatgga aaacactcga
     3901 ctttccgaaa gtcgctaaaa atgatttcgg actgaacgga attgaatttg tcaacacact
     3961 ttttgaagtg ccgacggaag gctacctcag acggctgaaa agaaatgccg gagacctggg
     4021 ggttaccatg gtactgataa tggttgatga tgaaggtgac ggctgctcgg ttaccagtga
     4081 agaacggaaa cagtttgtga caaatcacag gaaatggatt gatgcagccg tttacctcgg
     4141 atgccatgca atccggacaa actgcgtggg tccgaaacag atcgacagga acgaggctct
     4201 taaacatgct gcggaaacct acaacatgct gctggaatat gctgttccgc ttaacataag
     4261 tgttctgatc gagaaccacg gcgggctctc aaatgatgca gactggatgg tttctctcat
     4321 gaagacggta aataatctct atttcggcag ctacccagac tggagacagc cttcagacaa
     4381 tttcgacaac gtggattatc ttgtgaaaat gctgccatgg gccggcggca tgtcttaccg
     4441 taatcagccc accgaagaac tgacagccag aatgattaac ctggcaaaga gttccggcta
     4501 caggggctgg tacggaatag aatcgtcagg ccgtgaagaa ataaaaaaag gtattacatt
     4561 gctgaaaaaa tatctttaaa aaacaacata tgacaagaaa aatgcttctg ctcgcgatca
     4621 cagtcattac tgcctgctcc ggtccgggag gcggtaaaag ggaggctcag ccggcactgt
     4681 tcatcagtga agaaaccatt aagaccgcaa tcagggattt caaaccggaa tcaccggacg
     4741 ccgatattgc cctctttgag aagggagtga ggcatgctgc ctcactgtgg agggagagtg
     4801 acggaacagc cgctgagttt ctcagcttcg taaaggataa ttatatcagc gaccctctga
     4861 aacggagaac cgttttcaac aaaacaagtg attattttga atccctttat ggctatttca
     4921 atgaaataac acttagcctg agaaagaatc tggatgaagc ccggggggaa atagacggga
     4981 tagaccgtat gttcggcaat tatagtccgg gggcccatct gcaggatgat ctctattcca
     5041 acaagattgc atttgtaaca gctcttaact tcccctactt taccctggcg gaaaaagagg
     5101 agctcggccc ttcatggagc cgagagcagt gggcaatggc acggctggga gatatctttg
     5161 tctcaagaat tccggcagaa cttatccagg cactgtcagt ggcaacagga aatgccgaga
     5221 tgtatattgc tggttataat atccatatgg gacgtctgct gactgatgaa ggccggaaaa
     5281 tctttccgga cgacatggta ctcctctcac actggaatct cagggatgag atcaaggctg
     5341 actatgcgga caggctgaac ggccgggaga aacaggagat gatattcagt gtgatggaaa
     5401 gaataatacg gcaggagata ccggaggttg ttatcaataa cccggaaacg gactgggcac
     5461 ctttcacaaa cagggtaacc aaagatggca gaccccttga atcagcagct gagaacgata
     5521 tgcgctacca gcatatactc aatacattca gggcaatgaa ggctatagat ccctatgttc
     5581 cggaaatgaa tactgccatc ctccgcagtt tttcatggga tatggaaata tcacaggaag
     5641 aggttgaagc actttttgac aattacctcc gttcaccccg gcttgcgaaa atagccggtg
     5701 tcatcaggga acgcctggga cgggatctca ggccctatga catatggtac gacggattca
     5761 agtcaagaag cagcatatct gaagattttc tcagctccag aaccgcaaaa ctgtatccgt
     5821 ctccagaagc ctttcgtgca gggatgcccg ccatgttgaa aaagctcggc tggagcccgg
     5881 aaagagcgga atatatttct tcaaagatag tggtcgaccc ggcaagagga tcagggcatg
     5941 cctggggggc aggcaggaaa ggatcccttt cccgcctgcg taccagggtt ccggaaacag
     6001 gaatggatta caagggctat aacattgctg ttcatgaatt cggtcacaac gtggaacaga
     6061 ccattacact ctatgatgtt gaccattaca tgatgtcagg tgtgcccaac acggccttta
     6121 ccgaggcaac tgccttcatc ttccagcaaa gggacctgat gcttcttgga atcagcgatt
     6181 ccggtccgga aaaggaaaaa tcagaaatcc ttgacaatgc ctggtctctt atggaaatca
     6241 tgggtgttgg aatggtggac atgaaaatgt ggaagtggct gtatgaaaat cccggtgcat
     6301 cagcagctat gctcagggaa gccgtggtca gaattgcaac ggatacctgg aatgagtact
     6361 ttgcccctgt gatcggagta aaggataccc cgctgctggc aatatattcc cacatggtga
     6421 atgctccgct ttatcttgca aattattctt acggccatgt tatccagttc cagatcgagg
     6481 aattcatgaa agggaaaaaa ctgtcagacg aaatagaccg gatatataag cagggacgcc
     6541 tgactccgca gcaatggatg accggggctg tcggatcaaa aatttcagca cagcccgttc
     6601 ttgatgcggt tgacagggct ttgggaaatt aggagccggt gaacgaatgg gaaaagaaga
     6661 aatcaattca atacttgaag aacagcttaa gtttttcaga tcaggaaaaa cccttgatat
     6721 aaattttcgt cttgggattc tgaaaaaatt cagaacactg ataatatatc atgagaaaga
     6781 aatagcggat gcattatgga aggatttcca caaacccgaa ttcgaggtta tcgccacaga
     6841 aacgggtttt gtcctcaagg aactgaacct ggcaataaaa aagcttaaaa agtgggccag
     6901 gcctaaaaag gtaagaactc cgtttgttca ttttcctgcc cgtagttata tcactcatca
     6961 gccatacgga caggtacttg ttctttctcc atggaattac ccttttcagc tggttttcct
     7021 tcccatgctg ggagcaatta ccgccggaaa ttgcgttata gtgaaagcat ccggaaaggt
     7081 tccgaatatt accagcgtaa tgcagaagat aattggaaat tttcccgccg gactggtggc
     7141 aatggtaaac ggagatcatt caataagcga acaccttctc agctataaat tcgattatat
     7201 cttttttaca ggcagcacac ggattggtaa atacgtgatg caaaaagcat ctgaaaacct
     7261 tatacctgta tctcttgagc ttggcggcaa gaatccatgt gtcgtaacct cggatgcgaa
     7321 actcggcttt gcggcaaaga ggattgtttg gggaaaattc atcaacgcag gccaaacctg
     7381 tatctgtgct gattatctgc tggtagacaa aaaggtgaaa gatcgcctgc tagatctgat
     7441 atccgccggt ttaaaagaat tctacggcga aaatccggaa aaaagcaggg attttgcaag
     7501 gattatcaat gaagagaacc tgctgcggct tgttgccctc actaaaacag gacatatagt
     7561 tgccggcgga aactccgaca cagaaaccct ctaccttgaa ccaacggtaa ttaaggatat
     7621 cacacccggg gatctcatta tgcaggaaga gatcttcgga cctgtgctcc ctgtaataga
     7681 atttgaaaac ttaagtgaag tttataatat tattgaaaga aatccgaaac cccttgcagt
     7741 ttatatattc acccgcagta aaaaactggc ccgcgaattc ctggcaagaa caagttcggg
     7801 tacggctgct gtaaatgata ccgtgatgca gattgcctct ccctttcttc catacggtgg
     7861 agtgggatac agcggcatgg gcaggtatca tggcagaaga tcatttgaaa cattttcgaa
     7921 tacccggtcc gtactcgtaa aatcgaatct gatagatttg attttacgtt atcctcctta
     7981 cacaaaattc agcaaaaaaa taatcagatg gctgatgaac tgaaaattgt atcgggctga
     8041 ttcatacatt cccgctttct gcctgcagct tatattgctt caatggttga aggcggtttt
     8101 gctgttatat tataggtaac gcttaccaca cctggaacct ggtctgtaat gtcggcagcc
     8161 attttttcaa gaatttcgta tggcagacgt gagggggttg ccgttcttga atctgtactg
     8221 tcccagcatc tgacttcaat ctgctgtccg aaatcacgcc tgccctccct gacccctgtc
     8281 accctgtcgg aatgcagaat agccatatac tggaatgcac cgctgccggc aagagccttt
     8341 tcaacaatca ctgtagcctt tcttactgtt tccagccttt cagctgtcac ttcaccaatt
     8401 atcctggctg ccagtcccgg tcccggaaag ggtatcctgc ccagcatctc ttccggcagt
     8461 cccagggccc tgcctgcaat ccttaccccg tccttcctca gccgtacaag aggttctatt
     8521 atctgatagc ccagggcttc ctccggatct attccaagct gggcaaaaac attgtgctgc
     8581 cgcttgatgc ccgccacagt ttcatcaaca tcagtaagaa tcgtaccctg aagcaggaat
     8641 ctggcaccgc tttcccttac cagtctcccg aaaacccttt tataaaaggt atttgttact
     8701 gcctctcttt tctcctcagg atcagtcagt cctttaagtg aatcaagaaa ttccattctg
     8761 gcatcgatta tctcaacctc aaccccgagt tcatggaata ctgttgacac ccattcggtt
     8821 tcgccctcac gcaatagtcc gttctgaact attacagtcc ggagctgccg ccccagtgcc
     8881 cggtgcccca gcattgtcac cactgaagaa tcaaccccgc ccgagagagc attaattgca
     8941 ataccgtctc cgacagctgc ttttatcgac tctgcctgtt cctcaacaaa tttatccgga
     9001 tccagttcct taaggcctat ctcttgtatc attgaatgat tatttagtat ttacagctcc
     9061 gtttttctct cttcctgcag gatcatattt aaccagcccc gccccgcagt tcaggatccg
     9121 gttctttccg cctgacagca tcccgtctca caatccgttc cggtcaacgt gcctgccgga
     9181 tattcaacca cctcgtatac aggccggtat tgccggttca ggcggacttg ttcatatttc
     9241 cggcattgtt aaacccattc aaatatattt tgtttttttt atttaaatgt tatacatcag
     9301 cagatccttt gcattttccc cgactgtcga agcattattg ccataacatc agcaacttat
     9361 aattcctttt tttagctttc tcctgtaaat cctttccgaa ttacagcgcc cttgaggttg
     9421 cagattcctc ctttgagtat gattcaggcg ggacatccag gagtttcaga ccgcggaccg
     9481 gattttttat gtaccttcaa tattttctac ctttgtatat tctttgtgtt ctcatcaaaa
     9541 aattctgaat aaaaccagtg tatatgaaca acatacatga attaacaaaa tagtaaaaaa
     9601 cttatgataa aaacattcct gcctgccatt accgaagtga ctgcatgctg cagtcgctgt
     9661 tctgctaaaa aacacccggg gatatggatt tgaagtggcc ggaaccggtt acggagaggg
     9721 gcagggaaaa gagtataact tccaacttaa tcagttctga tccgtgacag tatgttatgc
     9781 gaaaaataga aaattattaa ttaccgggtt tatgagaaaa acaatttcag gtttttgtat
     9841 gatgttatta tcattagtga tatccgaaaa ggggattgca caggataata actggacgca
     9901 tttcagggga agtggtctta atggtatcgc aagtgtagaa actgttccac tgaactggaa
     9961 tgatgattcc ggtttcagat ggaagacaga catccatggc aggggctggt cttcccctgt
    10021 agtctatggt gatcagatat ggataaccac tgctactgat gacggcaggg aactttttgc
    10081 cgtgtgtgcc gatttccgga acggcaaaat aattcatgac attaaggtat tttccgtttc
    10141 tgatgttgaa gggaagcatt cactgaattc atacgctact cctaccccat gtatagaaaa
    10201 aggatttgtg tatgtccatt tcggcagtaa cggcaccgca tgcattaata cttccaatgg
    10261 caatatagtc tggaccagga ctgatctgaa atgcaaacat gtacagggcc cggcttcatc
    10321 tccggtactg tataaaaacc ttctcatcct tcattatgaa ggtactgatg tcagatttat
    10381 tactgccctg aacaaatcaa caggccagac agtatggaaa accgacaggc ctcaaaaacc
    10441 ttatgaacca ctcacggtca taggcaggaa ggcgtatgta acacctctta tactgaatgt
    10501 aaagggcaag gatctgctga taaccaatgg ttcggcagta tgcaatgctt ataatcctga
    10561 tactggtgag gaggtctgga gtctgatcgg gggagctgaa tcaaccattg ccatgccctt
    10621 ttcagaaaac ggcgttttat actattatac gggcttcatg agagatcctt caggaacgga
    10681 atattccgaa ttaatggcag taaatccgga tgggaaagga gatattacag ccagcaacat
    10741 aatatggaaa aaaagaagcg gcagacttca attgcttact ccggtcataa aagacggact
    10801 gatctatact gttgattcaa acaataacct gatgtgcatt gaagcagaaa cggggaaaga
    10861 ggtgtggaca acaagggtga gggctaattt caacgcatca cccgtcctgg cggcaggcag
    10921 gatctatttt ttctcggtaa ggggtgatgt acttgtgatc aggcccggaa ggaaatatga
    10981 agttttaagt cagttccaga ctggcaaccc ggtatgggca acaccggcgt ttcttaggaa
    11041 ttcgatcatt ctgcggacgg ataaatatat aggaaggatc gaatgatttt cccttcttct
    11101 acagccacga accttcaggt atcctgttat aaaccacagt atcttcaaac gcttcctttg
    11161 aagaatattt aaacttataa ccataccttg aagtaagttt tgtcgaatca acgattgtag
    11221 gataaaaact gtggacaatg gaagcaggct gaatattcat tatcttcaga tgccagcaaa
    11281 tccacaggaa tcctttaatc acgaaaaaag gaaccggcag gaatttttta tcgggaacaa
    11341 tgtctttgac tgtcgaataa ctgtcaggta caagattata aattcctgag atttcattat
    11401 ccttaagaat cagttccatt aaagctacca ggtcgacact gtggatgaac tggagttttg
    11461 tttcccgtgt gaatttcggc aggaagggta cctttataag gattgatact atgctttttg
    11521 gtttgtcaaa ggcaggaccc agtacctggc agggtctcag tatcactata cgcagatcat
    11581 cccggacttt aatattcgtg taattgtgtt caacaagctt tttgttcatt ccatacctgt
    11641 attttccggg atcgaggggc tcagattctg acatccacag gggattattc tttttccccc
    11701 cgtaagctgc agccgaagat atatatataa gttgcctgac agatggagtt atctgcgaag
    11761 ctttcaatac attatccgat cctgttatat ctatctcata ttcctgtttc cggttgcgca
    11821 ctttattgaa ggaacatgca aaatgcacta cgttggttgg cttctctccg gcaaatattg
    11881 aaataagttt ttctttctcg gtaatactgc atttataaaa ccggaactta tccccgtgta
    11941 aagagtcact gcattccctg atgtcgattc cgactatttc tgtatttttc tctgccaggt
    12001 ggccgatcag cattctggcc agagatccgg atgaacctgt tattaatacc ttcataataa
    12061 ttaaattaac cagacaatta atgtatcagt tcttatactg cctcgatact tgatggtggt
    12121 ttggctgaaa tattatatgt cacactgaca ataccgggta cctcatttat gatatctcct
    12181 gcaagtgttt caagtacttc gaaaggcagt tttgaagctt cattcccgaa agcctttatt
    12241 gccacaaaag aaacgcccgg ctgatccggg actaataata tctgccaata tatttatttt
    12301 caacaatgag ctttttcatt tcatgcggat cgatcatatc ctgtctggca taaacaatct
    12361 ttcctcccgg ctctgccagc agtgtgaaag ggagggctcc tctccagccg ttgccaacag
    12421 catcaataag cctgtaaaca tcatcttcac tgaagatata attccggttt gatgcctgct
    12481 gtgactggag gaacttcaat gcatcattct ttctgtcctg tttgtcggca ctaattgtta
    12541 tgaattcgaa atcacggccg caatacatac ggtcgatgat aaccagttcg ggaaattctg
    12601 ttttacaggg accgcaccat gttgcccata tattgatcag tctgagttta tccgaagaat
    12661 tattctgaat gagctccatt actccgttaa catcaatatc ttcaagtgtt acaggcagtt
    12721 ttgcccactg ttcatagagc ttttcagcat attcattttt ccatgcccac ttaatggagc
    12781 atccgaaaac c
//
LOCUS       DAOSPX010000059        12738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4950_length_12738_cov_4.341402, whole genome shotgun sequence.
ACCESSION   DAOSPX010000059 DAOSPX010000000
VERSION     DAOSPX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4950_length_12738_cov_4.341402"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2567)
                     /locus_tag="PLZ75_04600"
     CDS             complement(<1..2567)
                     /locus_tag="PLZ75_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HOS71712.1"
                     /translation="MTGLIIVLAAVCVYSCRNSSSDPWVLSAPAGSLYTSINKTGVTV
                     IPNGRLITPSGKTIDVAPHPFGLTLSRDGETAVTANSGIRPLSISIIRNLNTEPEIMQ
                     IPPGYNTDKGILESVFMGLAISPENDRVFVAGGQSNCIFVFDMLTGEKTDSINCAVSS
                     DNSRWPDGYIGDMVISSDGNFLYAVDQTNFRLLIADTRGMQVVRSVPVGRYPFGVALS
                     KDEKKVYVANVGMFQYSKISDIEEKSDYQKALKFPPFAYNTEEMRNGVVIDSLEIPGL
                     GDPNSDMAFSVWAISIEDPDNPEVISRIKTGNLIGQMVEGIPAVGGSSPNSLAVTSDY
                     VFTSNGNNDNITVIDIRSDTVVCDIYLKPDEAVKQFRGVIPFGLAVSPDEKKLYVAEA
                     GINAIGVIDLKSFRVEGHIPTGWFPSRIKVSPDGKNLIVTNAKGYGSGPNGGRSFTEG
                     GEGTYIGNLMKGSVSIIRIPSDRELRMMTSRVMANNFRKYRASKVLKARKDNPVPLYG
                     GQKESPIRHIVFISKENRTYDEVFGQVKSGRGDSSLARYGYNVTFSNRNNTRRVENAT
                     VMANHLKLAAEFAICDNFYVDADVSADGHRWLVNTYPNEWVETSVAASYGGNRNYNPA
                     SSAPGSLGMNGSAGAIYPEDYNESGSMWDHLERNNIEFFNFGFSVMFEPGFYHESYKY
                     EGIRQFVNFPLPTSLFSRTSRQYPTYNMAIPDQFRINQFIREFSEKWLSGKDSLPPVI
                     TVIVPNDHGADERPEAGYPFRESYMVDNDFAVGRLVEFLSNTPYWKNMAIFITEDDAQ
                     NGVDHVDAHRSILMVISPYVKKNYVGKVHYSFGSIFKTFWNILGVPYLNQYDAGATDL
                     "
     gene            complement(2715..4112)
                     /locus_tag="PLZ75_04605"
     CDS             complement(2715..4112)
                     /locus_tag="PLZ75_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOS71713.1"
                     /translation="MKSIHHRGKNPGTNIVSGTGKSRGTKNRRAAAALIIFLISAGSV
                     ISQDWPQYLGPQRNGTSPQKGILRSWPAKGPEILWTANIGIGYGGTVVRDGRVYLLDR
                     DDRTGDRLRCFDLSSGRELWSVGYDAPGTVQFPGSRSVPAIDGNRIYTCGPYGHLYCI
                     DINTRKPLWNKNIWTDFGGGEIPRWAISQCPLVYGNLLIVASQAPEAGVVAYDKLSGS
                     VRWTTASLGPVGYVSPALLKAGTQDHVVMITASAGRGPSASGGKVAGIDPLTGKILWE
                     YDKWQCGIPVPCAIDAGEGRVLITGGYQAGAAMLKISRKSDGSFEVTELFKNPDFGAH
                     TQPPVLHNGYFYAQYSTNERKDGLVCMSMDGQVKWSTRRSPAFDKGGMILAEGLLLST
                     DGSNRLYLIQPDPSAFRALASAEILSPGGTGTENDPVASRVGGATQNWAPLALSDGRL
                     IIRDQKRLICVRVAK"
     gene            4384..5310
                     /locus_tag="PLZ75_04610"
     CDS             4384..5310
                     /locus_tag="PLZ75_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71714.1"
                     /translation="MKKFTLSVLFCLIISSILTVYGQTIPREELIFLTSEWKGERFPD
                     GRPRISDDLIRRARNLSLEDAWQIMNNEGYSNQFEGNWKMVRDDLPIIGRAVTATFMP
                     SRPDIEKNIKERGWKNGFVGNTNSWPISVLQKDDVYVADCFGKIKGGTMIGDMLATSI
                     YTKTGTGVIFDAAARDLTDISEIKGFNAYVRDFHPSFLVEVVLTGLNTPTRIGEAIVL
                     PGDLVISDKEGVLFIPAHLAEMVIATAEFMSARADFSKAMLKEGRYSSGQVDSQWTDD
                     IKEHFLRWLKETKSETQMTRKQLDEFMSKRTW"
     gene            5409..6677
                     /locus_tag="PLZ75_04615"
     CDS             5409..6677
                     /locus_tag="PLZ75_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71715.1"
                     /translation="MKTKSDEIEANLDNPEILEKLYRSDKKGFRTEFEKLRPGTGFSE
                     IIRFWKVRLEYDKKSDTVKMPSVYEITTVLAAGIITAVLIKIPDIFRTGIDENMFYLK
                     NAAIIIFTGLTLYTILINRVSEPGKIIYTLLAFLVPAIYINLLPSSDPGASVILACIH
                     LPVLMWFIYGIAATGYDTGNLNKRADFIRFNGRLAIVYVLVAIAGALLAAVTIGLFDS
                     IDVNIEKFYTRNIIIMGAVAAPVAAAFISDKYPALVGKTAPLLAGIFSPLVLITLAVF
                     LATMLTAGKNPYKDRDFLLIFNIMLLGVTGIIIFSVFEAPVLRNQKFNAGVLLILSLI
                     TVITDLIALSAIFFRLGEYGLTPNRLAVLMSNILILINLILIMIDLIRISFKGRDFKI
                     VEMTTVKYLPVYMAWVIIVVFGFPAVFGMQ"
     gene            complement(6706..7047)
                     /locus_tag="PLZ75_04620"
     CDS             complement(6706..7047)
                     /locus_tag="PLZ75_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOS71716.1"
                     /translation="MRTAIDNIVPGNIIIIVSGLVSERGTTPDFDCGLATENMFIAAH
                     GLGLGARIYTGPVRDVNLNRELFQIPAGYNAVALLRIGNPDRNIDAVSSATPRKTPDE
                     LVNRKKKKSDP"
     gene            7418..8320
                     /locus_tag="PLZ75_04625"
     CDS             7418..8320
                     /locus_tag="PLZ75_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HOS71717.1"
                     /translation="MKISKFIEKYFWIFLIAGIVAGILLPLKSEAPPIVLKLLLGMML
                     FLVFIKIDALEVLENMKDLKLMALIAFIYMIAVPVLFYLMTRIFDRQLAIGLLLLTAM
                     PAGVSSPALVDLLKGNIPLAMSIAVVTQIIAPFTVPLLFWIIGLTGLEINILLVFRDI
                     VLLIFTPMILAQLCKNLLPGAISRGQHLFTSLNVFVLFSFVYVAISAQRDALMENPVS
                     LIWKTVILYLVFALLHAVGYLMGLRQNRENRIAYAVTSAYMNNGMAIVLAAAYFGPEI
                     LILMVLSELPWNTLPEPFRRITRM"
     gene            8590..10647
                     /locus_tag="PLZ75_04630"
     CDS             8590..10647
                     /locus_tag="PLZ75_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOS71718.1"
                     /translation="MSCGRKKQMMQPVLGSRSAAIIEQDGFKFKDLNKNGKLDKYEDW
                     RLSPEERSHDLLSQMSLEQKAGFMLINSLNMVGTARAGNSEGKLAPEDLDEGGPGRSA
                     IPGMERPGGRDQARGRQTGQRAPEGDAQNRNIQRFAAMAGTAGTSGMVKEFHNRHFIL
                     RANESAWITAEWANKLQALCESEPLGIPAIIASNPRNHITTNATIGTSVGTTVFSSWP
                     GELGLSAMRDLELTREFADIARQEWAAVGLRKGYMYMADLSTEPRWSRINGTFGENAE
                     WVAKMIREIVIGFQGEKLGSSSVALTTKHFPGGGAGEKGQDSHFEWGKREVFPGGMFK
                     NNLIPFQAAIDAGTSAIMPYYSLPAGTEYDEVGYAFNKGVLTDLLRNQMGFRGIINSD
                     TGPINNMPWGVENLTVHERYKKALEAGVNIFSGISDPKDLIEVIESGMVDMSLIDNSV
                     LLLLREKFELGLFENPYTDPDEAERIVNNEKFRERASLALRKSIVLLRNNNEALPLKA
                     GTKVYFENLHRGRNQEAGRKDVYLPSDNQYDLSFVDSPSAADIAVLWIQPAGNALFSS
                     TGEPISLSLSKCLIDVGYINNIISRKPTLLVINYTNPWVINEIYNEGAQNIAGVIATF
                     GTTPEALLDVISGKFNPSGRMPFSTPVSDEAVTNQKEDVPGYLEVEGYALFNYDEGLS
                     YSR"
     gene            complement(10650..11564)
                     /locus_tag="PLZ75_04635"
     CDS             complement(10650..11564)
                     /locus_tag="PLZ75_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS71719.1"
                     /translation="MDIDRRKFISTTITIIGGIPLASLIHAKDDFEPVCFGMFTDSHY
                     ADREPLHSRHYRESLDKLSECVDLMNELKVDFLIELGDFKDQGDPPDEKETLRFLDTI
                     EKGFCRFKGPHYHVLGNHDHDSITKGQFLNGISNSGFDRALPYYSFDTASFHFVVLDA
                     NYRPDGKAYGCGNFDWTSAFVPGRQLRWLEKDLAGTVKPTVVFIHQRLDINQENRIYG
                     PVNAAEVRKVLEDSGKVIIVFQGHEHAGASGRINNIPYYTLRGMIEGSGPESNSYATV
                     SIDRDLNINIKGYHRAVSDYLPPVSVPG"
     gene            11862..12266
                     /locus_tag="PLZ75_04640"
     CDS             11862..12266
                     /locus_tag="PLZ75_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71720.1"
                     /translation="MNDFLCPKCKDHLRVGDHIIFKVKNNEKKTALLLLNPQIGNYTS
                     TKHPSFIIKEGEFLEFYCPLCGASLKSDIHENLALVLMNDEKGNRYAVYFSQIAGEHS
                     TYKTTDDAVHMEGEDAGRYTYFRVGDRFKRYF"
     gene            complement(12314..>12738)
                     /locus_tag="PLZ75_04645"
     CDS             complement(12314..>12738)
                     /locus_tag="PLZ75_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOS71721.1"
                     /translation="VMWLFSHSGINNVNIDVAVAAREKGMKVIVFGSASEAAGRKSRH
                     SSGKTIFELADVVVDTCTPLEDASVPLKNHPDKIGPVSTMAFITCVWMTITTVAEILA
                     DRGVKLYIHPSHNVPGNESSRVRLDEALDEYKRRIAGV"
BASE COUNT         3481 a         3088 c         2846 g         3323 t
ORIGIN      
        1 agatctgttg caccggcatc atactggttg agatagggga ccccgagaat attccagaaa
       61 gtcttgaaaa tacttccgaa actgtaatgc acctttccga cataattctt ttttacgtaa
      121 ggtgatatga ccatgagaat actcctatgg gcatcaacat gatcaacacc attctgtgca
      181 tcatcctcag taataaatat ggccatattc ttccagtacg gagtgtttga caggaattcc
      241 accagcctgc cgacagcaaa atcgttatct accatatagc tttccctgaa gggataaccg
      301 gcttcaggcc gctcatcggc cccgtgatca ttgggtacga taacggttat cacaggtggc
      361 aggctgtcct ttccgctcag ccacttttca ctgaattccc tgataaactg gtttatcctg
      421 aactgatcgg gaattgccat attatacgta gggtattgcc tcgatgtcct gctgaaaaga
      481 gacgtgggaa gcggaaaatt cacaaactgc ctgatgcctt catatttgta ggattcatga
      541 taaaaacccg gttcaaacat cacgctgaaa ccgaaattga aaaattcaat attgtttctc
      601 tcaaggtgat cccacattga ccctgattcg ttgtagtcct cgggatatat tgctccggca
      661 gatccgttca taccaagact tccgggcgca cttgatgccg ggttataatt acggtttccg
      721 ccgtagcttg ccgccaccga tgtctccacc cattcattgg gataggtatt gactagccag
      781 cggtgtccgt ccgccgaaac atcagcgtca acataaaaat tgtcacatat ggcaaactcg
      841 gcagcaagtt tcagatgatt ggccattact gtggcatttt ctacccggcg cgtattattc
      901 ctgtttgaga aagtaacatt gtacccgtac cttgcaaggc ttgaatcacc ccttcctgat
      961 ttcacctgtc cgaaaacctc gtcataggtt ctgttttcct tcgaaataaa cacgatatgt
     1021 cggatcggag actccttctg ccccccgtaa agaggtacgg gattatcctt cctggccttt
     1081 aaaaccttcg aggccctgta tttccggaaa ttattcgcca ttacccgtga tgtcatcatc
     1141 cgcaattccc tgtcagaagg tattctgatt attgataccg atcccttcat cagatttccg
     1201 atataggtac cttcaccccc ttcggtgaaa ctcctgcccc cattgggacc tgatccgtat
     1261 ccctttgcat tggttacaat aagattttta ccatccggac ttacctttat cctggaggga
     1321 aaccatcccg tgggaatatg cccctcaacc ctgaagctct tcaaatctat gactcctatt
     1381 gcattgatgc ccgcttcagc cacataaagc tttttctcgt caggcgaaac ggcaagtccg
     1441 aaaggaatga cacccctgaa ctgcttaacc gcttcatcgg gcttcagata tatatcgcag
     1501 acaacagtat cgcttctgat atcaatcacg gtaatattgt cattgttgcc attgctggta
     1561 aaaacatagt cagatgtaac agccagggaa ttcgggctgg accccccgac agcgggaatg
     1621 ccctccacca tctgacctat cagattgccg gtctttattc tggaaatcac ttccggattg
     1681 tcaggatcct ctattgaaat ggcccatacc gaaaaggcca tgtcgctgtt aggatcaccc
     1741 aggccgggta tctcaagcga atcaatgaca acaccattcc tcatctcttc agtattgtag
     1801 gcaaagggag gaaattttaa agctttctga tagtcggact tctcttctat atcacttatc
     1861 ttgctgtact gaaacatgcc gacgtttgca acatatacct ttttctcatc cttcgacagg
     1921 gcaacgccaa aaggataacg gcccacagga actgatctga caacctgcat gccccgcgta
     1981 tcggcaatca gaagacggaa gtttgtctga tccaccgcat aaaggaaatt accgtccgat
     2041 gaaatgacca tgtctccgat atagccatcg ggccatctgc tgttgtcaga tgaaactgca
     2101 cagtttattg aatccgtttt ttccccggtc agcatgtcaa aaacaaaaat gcagttgctc
     2161 tggcccccgg caacaaaaac cctgtcattt tcaggtgata tggccagacc cataaagact
     2221 gattccagta tccccttgtc agtgttatat cccggcggga tctgcatgat ctccggttcc
     2281 gtattcagat tccttattat tgaaattgaa aggggacgta tacctgagtt ggctgtaacg
     2341 gctgtttccc cgtcacggct cagtgtcagg ccgaagggat ggggagcaac atcaatggtt
     2401 ttccccgatg gcgtgatcag ccgcccgtta ggtatgacag tcacccctgt cttatttatc
     2461 gaggtgtaca gggatccggc aggggcagaa agcacccaag gatcgctgct gctgtttctg
     2521 caggaataca cacatactgc tgcaaggact attatcaagc ccgtcaatcc ggaaagtttt
     2581 atagtgttca atgtataatt gtttgtcaga ttaattttta tgaaaacatc agcactgaaa
     2641 ccggaaaata tatactgcaa tgaatccggg actgaagcaa agcatgaagc cggtcctgca
     2701 gccataaacc gtgatcattt cgccactctt acacatatta atcgtttctg gtcgcgtatg
     2761 atcaggcgcc cgtcactcag tgccagggga gcccagttct gggtagctcc cccgactctt
     2821 gacgctaccg gatcgttttc tgttccggta cctcccggac taagtatctc tgccgaggca
     2881 agtgccctga aagccgaagg atcgggctga ataagataaa gcctgttgct tccgtcggtg
     2941 ctgagcaaaa gaccttctgc aagtatcata cctcccttgt caaacgcggg agatctcctt
     3001 gttgaccatt tgacctggcc atccatgctc atgcagacca ggccgtcctt tctttcattg
     3061 gttgaatact gtgcataaaa ataaccgtta tgtagaaccg gaggctgtgt atgggcacca
     3121 aaatccggat tcttaaaaag ttccgtcacc tcaaagctcc cgtctgactt ccggcttatc
     3181 ttcagcattg cggcaccggc ctggtatccg ccggttataa gcactctgcc ctctcctgca
     3241 tcaattgcac aaggaacagg gattccgcat tgccacttgt catattccca caatattttg
     3301 cctgtaagag gatctatccc ggcaaccttt cctccgcttg ccgacggacc ccggccggct
     3361 gatgcagtta tcattacaac atgatcctga gtgcctgctt tcagaagcgc aggacttaca
     3421 tagcctacag gtcccagtga agctgttgtc catctgacac tgccgctgag cttgtcatag
     3481 gcaaccactc ctgcctccgg agcctgtgaa gctacaataa gcagattgcc gtaaacaaga
     3541 ggacactggg atattgccca tctcggaatc tcccctccgc cgaagtcggt ccatatattc
     3601 ttgttccaca atggttttct tgtgttgata tctatgcaat acagatgccc gtatggtccg
     3661 caggtataga tcctgttacc gtcaatagcc gggacactcc ttgaaccggg aaactgaacc
     3721 gtacccgggg catcatatcc gacactccac agctccctgc cactggaaag atcaaaacac
     3781 ctcagcctgt ctcccgtcct gtcatccctg tcaaggaggt aaaccctgcc gtccctgacc
     3841 accgtacctc cgtagccaat tcctatgttt gcagtccaga ggatttcagg ccccttcgcc
     3901 ggccatgaac gcagaattcc tttctgcggg gaggtgccgt tcctctgcgg accaagatac
     3961 tggggccagt cctgtgaaat aacactgcct gcactgatca ggaaaataat cagcgcagca
     4021 gcagccctgc ggtttttcgt tccccggctt ttgccggtgc ctgaaacgat gttggttccc
     4081 ggattttttc cccggtgatg aatgcttttc atccggtaag aaagatttat aaaataatgc
     4141 agtattgttt tcacaataat aaatcaatta cttaaaataa acaagataag gatacttcac
     4201 acctgcatgg ctccgttttt cccggcaggg aatggcctga tccttttaaa accaggtgaa
     4261 tcctcatgcc tgaaacaact ggtaaattca ttatttcact tataataaat ttcttgctat
     4321 ttttggaaaa tatcagtttt aatttcaaat agtaaatatt tataaaacct aaattttgta
     4381 tcaatgaaga aatttacatt atctgttttg ttttgcttaa taatcagttc tatcctcact
     4441 gtatacgggc agaccatccc acgggaagaa cttatttttc tgacctcgga atggaaggga
     4501 gagcgctttc ccgacggccg tcccagaatc tctgacgacc ttatcagaag ggccagaaat
     4561 ctcagtcttg aagatgcatg gcagatcatg aacaatgaag gatattcgaa ccagtttgaa
     4621 gggaactgga aaatggtccg cgatgatctt cccattattg gcagggctgt gacagccact
     4681 tttatgccaa gccggccgga tattgaaaaa aacatcaagg aaagaggctg gaaaaacgga
     4741 tttgtaggca acacaaactc ctggcccata tcagtccttc agaaggatga tgtgtatgtt
     4801 gccgattgtt ttggaaaaat aaaaggcggc accatgatcg gggacatgct tgcaacctcc
     4861 atctacacaa aaaccggaac cggtgtaata ttcgatgctg ctgcacgcga tctgacagat
     4921 atatccgaaa tcaagggctt caatgcttat gtacgtgatt tccacccttc atttcttgtt
     4981 gaggttgtac ttaccggtct gaacactccc acaaggatag gagaagccat tgtactgccg
     5041 ggagatctgg taataagtga caaggaagga gtgcttttca tcccggcaca tctggccgag
     5101 atggtcatcg ccacagcaga attcatgagt gctcgtgccg atttcagtaa agccatgctg
     5161 aaggaaggac gctacagttc aggtcaggtg gattcgcagt ggacggatga cataaaggaa
     5221 catttcctca gatggctcaa ggaaacaaag agcgaaaccc agatgacccg caaacagctt
     5281 gacgaattca tgtcaaaaag aacctggtaa gagcttccgt gcctccaacc ccagatggca
     5341 cgcaaacagc ccgaggagtt cataccgtaa gaacccggga gaaactcccg ggttccggta
     5401 aaaaagccat gaaaacaaaa tccgatgaaa tagaagccaa tctggataat cctgaaattc
     5461 tggaaaaact ctatcgttcc gataaaaaag ggttcaggac tgaatttgaa aagctccgcc
     5521 cgggaaccgg attttcggaa ataataagat tctggaaagt aagacttgaa tatgacaaaa
     5581 agtctgacac ggtaaaaatg ccttcagtgt atgagatcac tacagtgctt gcagccggaa
     5641 tcatcacagc tgtcctgatc aagatcccgg atattttccg gacaggaatc gatgaaaata
     5701 tgttttattt gaagaatgcc gcgatcataa tatttaccgg acttaccctt tataccatac
     5761 tgataaaccg tgtttcggaa cccggtaaaa ttatttatac cctgttggct tttcttgtcc
     5821 cggcaatata cataaacctt cttccatcat ctgaccccgg cgcatctgta atccttgcct
     5881 gcatacatct tcccgtactt atgtggttca tatacgggat cgcggcaacc ggatatgata
     5941 ccggcaacct gaataaacgg gcagatttca taagattcaa tggcaggctc gcaattgtgt
     6001 acgtgttggt tgcaatagca ggagcactcc ttgcagcagt tacaatcgga ttatttgatt
     6061 caattgacgt aaatattgag aaattctata cccggaacat aattataatg ggtgctgtgg
     6121 cagcacctgt tgctgcagca ttcatttctg acaaatatcc ggcccttgtc ggaaaaacag
     6181 ctccgctcct agctggcatt ttcagcccgc ttgtcctgat aacacttgca gtattcctgg
     6241 caacaatgct tactgcaggc aaaaatccct acaaggaccg cgatttcctg ctgatcttta
     6301 atattatgct tctgggggtg acaggcataa tcatcttttc ggtttttgaa gcgccggttt
     6361 taaggaatca gaaattcaat gcaggagtat tattgatttt atcgcttatt acagttataa
     6421 cggatctgat cgcgttgtct gccatctttt tccggcttgg ggaatacgga ctgactccaa
     6481 acagactggc tgtacttatg tcaaacattc tcatcctgat aaacctgatc ctgataatga
     6541 ttgatctgat cagaatcagt ttcaaaggca gggattttaa aattgtggaa atgacaacag
     6601 taaaatacct gcctgtctat atggcatggg taataattgt tgtcttcggg ttccctgctg
     6661 ttttcggcat gcagtgaaaa ccatggatgc gggaaatcct gagttttaag ggtcagactt
     6721 tttctttttc cggttgacaa gctcatcagg cgttttccgg ggagttgccg acgaaacggc
     6781 atcaatattc ctgtccggat ttcctatccg cagcagtgcc actgcattgt agccggcagg
     6841 aatctgaaat aattccctgt tcaggttgac atcccttacc gggcctgtgt aaatccgcgc
     6901 ccccagcccg agtccgtgtg cagcaatgaa catattctcc gtcgcaagac cgcaatcgaa
     6961 atcgggtgtt gtcccacgct ccgaaaccag tccggaaacg attatgataa tgtttcccgg
     7021 aacaatattg tcaatcgcag tccgcatggt ttctatgtcc cgtataactg tgaatttcca
     7081 tggctgcagg tttctgccac tcggcgattt gattccgcat gccagaataa tatcaagctg
     7141 ctggtttata tttatggaca gtaatttatg aaattttctc catttttacg gaatgactga
     7201 ctgattcttc taaaaatacc cgctttcaca gatactgcag cagccatccc gggttaattt
     7261 ttgcgctata tttcaaaaag aaaaaataaa ttttcatctt cgccagccgg aaattaaatt
     7321 cacagtccgg aatgacaggt ggaacagatc ctgggaacag atcctttaaa aatccggtgt
     7381 ccgttataac aataacaaat cattcagaca gaggaagatg aaaataagca aattcattga
     7441 aaaatatttc tggatctttc ttatcgcggg aattgttgct ggtatactgc tgcctctcaa
     7501 atctgaagct ccgccgattg tactgaagct cctgctcgga atgatgctct tcctggtatt
     7561 cataaaaatt gatgcgcttg aggttcttga aaacatgaag gatctgaaac tgatggccct
     7621 gattgcgttt atttatatga tagcagtgcc tgtcctgttt tatctgatga caaggatctt
     7681 cgacaggcag ctggcaatcg gattactcct gcttaccgca atgccggcag gggtctcatc
     7741 ccctgccctg gtggatctgc tgaaaggaaa tatacccctt gcaatgagca ttgcagtggt
     7801 gacacagatt atagcccctt ttaccgtacc tctgctgttc tggattatcg gattaacagg
     7861 ccttgaaatc aatattctcc tggtgttcag ggatatagtc cttctgatct tcacgccgat
     7921 gatacttgca caattatgca agaacctttt gccgggtgca ataagccggg gtcagcatct
     7981 tttcacttca ctcaacgttt ttgttctctt ttcttttgta tatgtagcca tttcagccca
     8041 gagggatgct ctgatggaaa atcccgtcag cctgatctgg aaaacagtga tattatacct
     8101 tgtttttgcc ctgcttcatg ccgttggcta tcttatgggc ctgaggcaga acagggagaa
     8161 caggattgcc tatgcagtta catcagccta tatgaacaac ggtatggcca ttgtcctggc
     8221 agcagcctat ttcggaccgg aaatactgat attgatggta ctgtcggagc ttccctggaa
     8281 taccctgccc gaacccttca ggagaatcac caggatgtaa atcctcttgc tcactttgtc
     8341 acctgtatct gctaccccgg caccttcaca ggaatgttgc aggcatatgc cctgtaacct
     8401 gacagattaa gtaatgagtg accggatcac agtccggatc tcccggcaac cggatattca
     8461 tttctgtaat gaatgaaaag gaaaaagtta tctttgcaga catatgaaaa ttacaggcaa
     8521 aaccataaac tgatcatgaa aaattattta accaatgcag ttcttatatt gttagttatc
     8581 tcaggcttaa tgtcatgtgg cagaaagaaa cagatgatgc agcctgttct gggcagcagg
     8641 tccgcagcca taattgaaca agacggcttt aagttcaagg atctcaacaa aaacggaaag
     8701 cttgacaaat atgaagactg gcgcttgtca ccggaagaaa ggagtcatga tcttctttcg
     8761 cagatgtcgc tggaacagaa ggcgggtttt atgctgataa attcgctgaa catggtcgga
     8821 acggccaggg ccgggaattc agaaggcaaa cttgcacctg aggaccttga tgaaggcggg
     8881 ccggggagat ctgctattcc gggaatggaa aggccgggcg gaagggacca ggcaagggga
     8941 aggcagaccg gacagagggc cccggaaggg gatgcacaaa acaggaatat ccagcgtttt
     9001 gccgccatgg caggcacagc cggaacatcc ggaatggtaa aggaattcca caaccgccac
     9061 ttcattctca gggcaaatga gagtgcatgg ataacagccg aatgggcaaa caaactacag
     9121 gcactctgtg aaagcgaacc cctgggtatc ccggcaatca tcgcctcaaa cccccgtaac
     9181 catatcacta cgaatgccac cataggcaca agtgtcggca caacagtctt ttcatcatgg
     9241 ccgggtgaac tgggactgtc ggcaatgcgc gacctggagc tcacgaggga gtttgcggat
     9301 attgcccgcc aggaatgggc agcagtcggc ctcagaaaag gttacatgta catggctgat
     9361 ctctccactg aaccaagatg gtcaagaatt aacggcactt tcggagaaaa tgccgaatgg
     9421 gtggcaaaga tgatcaggga aatagtaatc ggattccagg gtgagaaact gggatcttca
     9481 tctgttgccc ttactacaaa gcattttccc ggagggggag cgggggagaa aggacaggat
     9541 tcacatttcg aatggggcaa gagggaagtt tttcccggcg gaatgttcaa aaacaatctt
     9601 atcccattcc aggctgcaat tgatgccggt acttccgcaa tcatgccata ttattccctt
     9661 cccgccggaa cggaatacga cgaggtcggc tatgctttca acaagggggt acttactgac
     9721 ctgctgcgca atcagatggg attcagggga atcatcaatt ccgatacggg acccatcaac
     9781 aatatgccgt ggggcgttga aaaccttaca gttcatgaaa gatataaaaa ggccctggaa
     9841 gccggggtga acatcttctc cggcatttcc gacccgaagg accttattga agttattgaa
     9901 tcaggaatgg ttgacatgag cctgattgac aattccgtcc ttctgctgct cagggaaaaa
     9961 ttcgaactcg gactgttcga gaatccctat acggatcctg atgaagccga aagaatcgtc
    10021 aacaacgaaa aattccgcga gagagcctca ctggcattgc gcaaatcaat cgttcttctg
    10081 agaaacaata acgaagccct gcctctgaaa gctggaacca aggtatattt tgaaaatctg
    10141 cacaggggaa gaaaccagga agccggccgg aaggatgtct atctcccctc agacaaccag
    10201 tatgacctga gcttcgttga cagtccctca gcggcagata ttgcagttct ctggattcag
    10261 cccgcgggaa atgccctctt cagctcaacc ggggaaccga tctcgctttc tctttcaaaa
    10321 tgtttaattg atgttggtta tataaacaat ataatttcac gtaaaccaac tctcctggtg
    10381 attaactaca caaatccctg ggtcattaat gagatttaca acgaaggagc acaaaatata
    10441 gcaggtgtta tcgcaacttt cggaaccacg ccggaagccc tcctcgatgt gataagcggg
    10501 aaattcaatc cttccggcag aatgcccttc tccacccctg tttccgatga ggcggtcaca
    10561 aaccagaagg aagacgttcc gggatacctg gaagtggaag gatatgcact cttcaactat
    10621 gatgaaggcc tgagctattc acggtaaact cagcccggca cggaaaccgg cggcaggtaa
    10681 tcagatacag ccctgtggta acccttaata tttatgttaa ggtcccggtc gatgctgaca
    10741 gtggcataac tgttgctttc aggtccggaa ccttcaatca tgcccctgag agtgtaatac
    10801 ggaatattgt ttatccggcc tgaagccccg gcatgttcat gtccctgaaa cactattatt
    10861 accttgcctg aatcctccag tacctttctg acttcagcag cattaaccgg tccgtaaatc
    10921 ctgttttcct ggttaatgtc aagacgctga tgaatgaata caacagtggg tttaactgtc
    10981 ccggccaggt ccttctcaag ccacctgagc tgcctgccgg gaacaaatgc actggtccag
    11041 tcaaaattcc cgcatccgta ggctttgccg tccggtctgt aattggcatc caggacaaca
    11101 aaatgaaaag aagccgtatc aaaggaataa taaggcagtg ccctgtcaaa gccgctattg
    11161 ctgataccat tcaggaactg acccttggta atggaatcat ggtcatggtt ccccagtaca
    11221 tgataatgcg ggcctttgaa acggcagaat cccttttcaa ttgtgtcaag gaacctgaga
    11281 gtttccttct catcgggagg atccccctga tccttgaaat ctcccagctc aataagaaaa
    11341 tcaaccttca gctcattcat cagatcaaca cattccgaca atttatccag ggattccctg
    11401 taatgacgcg agtgaagggg ctccctgtca gcatagtggg aatcggtaaa catcccgaaa
    11461 cagaccggct caaaatcatc tttggcatga atgagggatg caaggggaat accaccaatg
    11521 attgtgatgg tggttgaaat gaattttctg cggtcaatat ccatatgaaa cagtctgtga
    11581 gattattatt aatattattc agagccttcc ctgttcagca ggacagccgg cgtcagctca
    11641 cggggattgc accgaataat atcttatcag gtgaaattac gataaatttg caagttcaga
    11701 gtcagaattc acgtgatatt ttttcgcgga agcctgaaac ccgggaatca tccacgggca
    11761 aagaacgggg aaggaagggt atgaatgctt atgattacat aattcatgct aatttcttac
    11821 ctttatattg ttaagatcaa tcacggaaaa aaggaaaaga aatgaacgat tttttatgcc
    11881 cgaaatgcaa ggaccacctg agagtaggtg accacatcat tttcaaggtg aagaacaatg
    11941 aaaagaagac tgccctgctt cttttaaatc ctcagatagg taattatacc agtactaaac
    12001 atccttcttt cataataaag gaaggagagt tcctggaatt ttactgtccc ctctgcggtg
    12061 cttccctgaa atcggatatt catgaaaatc ttgcgcttgt cctgatgaac gatgagaaag
    12121 gcaacaggta cgcggtctac ttttcacaga tagccggaga acacagcacc tacaaaacaa
    12181 cggatgacgc cgttcatatg gaaggagagg atgccggaag atacacctat ttcagggttg
    12241 gggatagatt caagagatat ttctgattgt gaaatccggt atgaaacctg taatggatac
    12301 agcctgaagg caatcagact cctgctattc ttctcttgta ttcatccaga gcctcatcca
    12361 gcctcacacg ggaactttca ttccccggaa cattgtgtga gggatgaata taaagcttca
    12421 cacccctgtc ggcaagtatc tcagctacag tggtaattgt catccataca caggtaatga
    12481 atgccatcgt cgaaaccggt ccaatcttgt cagggtggtt cttcagcgga acggaagcat
    12541 cttcaagggg agtacaggta tccaccacca catccgcaag ctcaaaaatg gttttgccgc
    12601 ttgaatgcct tgatttcctt cccgcagctt cagatgccga tccgaaaaca attaccttca
    12661 ttcccttctc gcgggcggca acagccacgt caatattgac attgttgata cccgaatggg
    12721 agaaaagcca catcacat
//
LOCUS       DAOSPX010000060        12719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4959_length_12719_cov_3.890714, whole genome shotgun sequence.
ACCESSION   DAOSPX010000060 DAOSPX010000000
VERSION     DAOSPX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4959_length_12719_cov_3.890714"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..599
                     /gene="efp"
                     /locus_tag="PLZ75_04650"
     CDS             33..599
                     /gene="efp"
                     /locus_tag="PLZ75_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOS71722.1"
                     /translation="MATTADFRNGLVIEFNNDLFTIVQFQHVKPGKGPAFVRTKLKNI
                     KSGRVLDNTFTAGVKINVARVERRPYQYLYKDEVGFYFMHLDTFEQIHVEEHVIDYPE
                     FLMEGQNVEVVAHADTENILSVELPQFIVFEVTYTEKGLKGDTATNAQKQATLENGSV
                     IMVPLFINNGDKIKVDTHNRAYMERVKG"
     gene            613..1827
                     /locus_tag="PLZ75_04655"
     CDS             613..1827
                     /locus_tag="PLZ75_04655"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOS71723.1"
                     /translation="MKTRSNRLQISRFKLGALLDITMAINANLPTETLLAKYESILRD
                     DLGIGKVLIFKLSDSWECLLNAGFQEELESLNVEEKLLGFEEITFVSADMDFPGIDII
                     VPVYNNNIQIAFLFIGDIEEEGEGMSPVLKHLNFIQTISNIIIVAIENIRLQKERLAQ
                     EALKKELELAARMQKMLIPDNRQLPKRPEISVHGFYYPHHEVGGDYYDCIRLSDTKTG
                     FCIADVSGKGMSAAILMSNFQASFRALFSAESDLEELLYKLNSIVIKNAKGEKFITFF
                     VARYDHVTRSLEYINAAHTPPVTYNTATGETDFLNTTCVGVGMLDSIPSIRTRSINIS
                     GHFKIVCYTDGLSELKDDFGFETGTREIINFISNERSVEQNMKDMLDHLGIPDSNPYL
                     FDDVSILAADLT"
     gene            complement(2146..3435)
                     /gene="tyrS"
                     /locus_tag="PLZ75_04660"
     CDS             complement(2146..3435)
                     /gene="tyrS"
                     /locus_tag="PLZ75_04660"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOS71724.1"
                     /translation="MNFVEELRWRGMLHDITPGTEEMLEKETAKGYIGFDPTADSLHI
                     GHMVQVMLLVHFQRAGHIPVALVGGATGMIGDPSGKSEERNLLDETTLRKNQEGIKKQ
                     LSKFLDFGSGRKNMAIMVNNYDWMKEFSFLEFIRQIGKHITVNYMMAKDSVKKRLGSE
                     GEGMSFTEFSYQLVQGYDFLHLYNEIGCRLQMGGSDQWGNIVTGTELIRRKTGGEAFA
                     LTCPLITKSDGAKFGKTESGNVWLDPERTSPYHFYQFWLNVSDEDAARYIKIFTLLGR
                     DEIEELIGEHSKSPHERLLQKRLAEEVTVLVHSREDYQLAVEASQILFGKGTTETLRK
                     MNETTFLSVFEGVPSFDIDRGILSAGAGFTALCAEHSAIFSSRGELRRLVQGGGVSLN
                     KVKIDDPDIVVGQDQLLNGKYLVVQKGKKNYFLLRFV"
     gene            complement(3476..4042)
                     /locus_tag="PLZ75_04665"
     CDS             complement(3476..4042)
                     /locus_tag="PLZ75_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOS71725.1"
                     /translation="MNWIDLIIVIVIIVAIIRGFTDGLIKEVAALAALILGIWGAVKF
                     SSFTAVKLYEWFDMTGQFVGVLAFIITFGVIMIIIHFVGIIVDRIMEAASLGFANRLL
                     GMLFGFLKSVLIMSVIFVILNALDSMKSFLPKKTISNSRFYIPISDVAPMIFPVLGEG
                     SYKRSFDRFTPPPEEKPKKERDPSELSI"
     gene            4669..4917
                     /locus_tag="PLZ75_04670"
     CDS             4669..4917
                     /locus_tag="PLZ75_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS71726.1"
                     /translation="MDPVDFTLYPNPAMDNTWLVLGEEMKSYTITIYNLSGTPLSVIT
                     GYASQSVWLDLKNYPVGIYIIKVTSGIRTGSKRLILNH"
     gene            4951..5568
                     /locus_tag="PLZ75_04675"
     CDS             4951..5568
                     /locus_tag="PLZ75_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOS71727.1"
                     /translation="MKKYMILTVLVINLLPLAGQNKIGVSFNQIYSTFRFKNSEGEIE
                     DLKYTIKYGYDIIWQYKILEVFFVEGSLSYNCKGASSTLGVQKLDWSLHYANAGVNAA
                     YKMTIGRLSPHGGAGFYYGRLLKADQLIGSDYYNLMETNYIRKSDIGTNIFAGIAYDY
                     SENGSVFIRLNEAVGLLQLEKNDLSSQRMFNRTFSIQVGLFFSIQ"
     gene            5582..7735
                     /locus_tag="PLZ75_04680"
     CDS             5582..7735
                     /locus_tag="PLZ75_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71728.1"
                     /translation="MKKRLSLLFAILGIAISLSAQIADGITYQAVALDDKGKEIAGHD
                     INGLIIHSKQISVRFSILRNSPDGELLYKEVHSTYTDPHGLFTLVIGHGEVSPDGMHQ
                     RLTEINWGAGRLFLMVEIDIKSRGNFKVMGVQQMMAVPFAFHSLSSSTVKYDDILNVP
                     QLSAVATTGNYSDLINTPSVFNGDYNELTNKPVIPAKTSELINDAGFINNYYETDPAW
                     RASLPYYYTKEDLRNADSSQIHFRNIINTPKTLAGYGITDAMSKSAPANRISSSDIAN
                     WNTSFSWGNHTGLYRLSSWLPSWNEIRANPFIIISPSNNQLLRYNSTTGKWQNWTPDY
                     LSDYTESDPVWTSASINYYTKTNLQTSGGSLVHFNNIISRPTTITGYGITDAMRTSHP
                     ANNITVANILNWNTAFSWGSHIGLYRPVTWLPSWNDLTANPFTITAPLNNQILRYNSS
                     TGKWQNWTPDYLTGYTESDPLWTSVSVNYYTKMNLEMPGSSRINFANIINRPGNLAGY
                     GITDAMVITHPANAITPSDIGNWRTAFSWGNHAAAGYQPFIVPGEIHHYLRGDMTWQP
                     LTAEAVGLGNVENISLHGWMGSPSLSVLGFVSQGEWHAGLIDASGPVMADLIIKKGGS
                     NREFLKADGSVDSNTYMIQTTEITDEFSASDGQTNFTLTRYPSAFSRIKMFVNGVRIS
                     NTAYSVSGQAITYISANNGSYELVGGDRIQFDYSY"
     gene            7883..>8637
                     /locus_tag="PLZ75_04685"
     CDS             7883..>8637
                     /locus_tag="PLZ75_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71729.1"
                     /translation="MPSLNRYGQELFKVTVTTFPVTSVTSNSAESGGRIENPDNIEII
                     ACGVCWSTSPGPDISDYKTSENTDVTEFTSHLTRLIPSTTYYLRAYVTTIAGTAYGEE
                     ISFRTLPRTDPSECLIAWYPFNGNADEATGFGIDGIVNGAVLSEDRFGNTESAYYFNG
                     IDNSITIPGYLPVTNTFTLSFWAYNEKVKGSANIITDGSRNIGGMDFLLNFRGNEIGI
                     RADKGSLLNYEYSSPAELRNLNLLNRWVHVAWV"
     assembly_gap    8638..8737
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8764..11529
                     /locus_tag="PLZ75_04690"
     CDS             8764..11529
                     /locus_tag="PLZ75_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOS71730.1"
                     /translation="MESNIISDGGTEVTERGVVWSTSLNPTIHDNKTSDGSGKGSFTS
                     HITGLLPGTTYYLKSYAINNAGTFYGNEWSFRTHDTLSNCGTVTDIDGNIYNTVTIGN
                     QCWMKENLKTTRYSDGTPISLVDEQNIWSKLSISDKAYCWYNNDINNKNPYGALYTWA
                     AAMNGADSSTSIPSGIQGVCPTGWHLPSWSEWGILIQYLDPQIVDPLYLRGSSIAGGK
                     MKESGFTYWDYPNTGATNESGFSGLPGGGRSEDGEFLFLGNSSYWWSTSQFGSGNYTR
                     VQNLNYNNSSNQMTSTPWKSGISVRCIKNVDQLIQLPVITTTQISHISDTSATSGGKI
                     INNGGDEIIARGICWSLSTNPTTTNNKTNSGTGTGLFIDHIGPLTPGTTYYVRAYATN
                     SAGTAYGNEEVFVTCDYIPPVAFNSNLAYGVITDIEGNFYKTIKIGNQEWMAENLRTT
                     KYNNGESIPVITDNDTWQSLSEGALCWLNDNICYKNTYGGIYNWYAANNNLCPVGWHL
                     PTNEEWSVMNRALGGNEIAGGKLKEQGFSHWSEPNTGATNESGFSALPTGGFRNGALF
                     VTGEFASFWTSTEYPDNQAFYRSLQHQYSIEGYSWGYKSNGANVRCLKGPAPPKPSVT
                     TLWARNIMSDKAEIVSVISSVLPVNSRGVCWNTSPAPEVLDNKTTNDGGTGSFTSLLT
                     GLESNTTYYVRAYSINDDGITYGNEITFSTVEDLPIGGLAAYWPFNGNANDESGNNNN
                     GTVYGATLVADRFGNPEKAYYFNGISNKIYIGNSLIVQGHITVACWIKSTDIGNQIIS
                     SGSRNSYRIGKTKDGVDAAVYLTDSSDGYGLDVHAVTDCSDWTHVAIVYNGVSLDLYV
                     NGQLMDSAYGPGNVFCNQPVYLNFGTYQWLSTYYGFYQGYLDDVYMFNRALSNSEIEA
                     LYHEGGW"
     gene            11611..>12719
                     /locus_tag="PLZ75_04695"
     CDS             11611..>12719
                     /locus_tag="PLZ75_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71731.1"
                     /translation="MKNLFKSVLIALLVYSMLSCSSDKEKTESFTTRSVEENKATIES
                     AGIDLIGVLNRMRSTQTVEAIGNMIQLTGSGKSKGIGDFTGTGLYSVFRSIHGCAART
                     LQVNDVFESIKGCADEDPESIQEFWDRNVGTYTWNSSASDWDISPGGDKFIIKFPSSG
                     NSKMNNATFTIYNYEGVIVENPFDEEYTGDFPASLQADLKIGSQTLLRFVFGASYNAD
                     GIPNAIACDLDFEGFKFEIDVANDTRVISASYKFMEGKAVIMKLSAVVNGLFTADNLE
                     LNTVHHSESHSYVCDFVYNPETKEYVEVYCEYTNEWEKIEFEEIAHSANAEFQLMNLA
                     VRGDIDIKKLVDKIRIIEDQYDTEQIEEEEYFNSIS"
BASE COUNT         3898 a         2586 c         2663 g         3472 t
ORIGIN      
        1 aaaaaagaaa taaataagga agaataaaga ttatggcaac cacagcagat ttcagaaacg
       61 gactggttat tgaattcaat aatgaccttt tcaccattgt tcagtttcaa catgttaaac
      121 ccggcaaggg gccggccttt gtaaggacca agctgaaaaa cataaaaagc ggaagagtgc
      181 tggacaatac ctttaccgcg ggtgtgaaaa tcaacgttgc cagggttgaa cgaagacctt
      241 accagtatct ttacaaggat gaagtgggat tctatttcat gcatcttgat acctttgaac
      301 agattcatgt tgaagaacat gtaatagatt atcccgagtt cctcatggaa ggccagaatg
      361 ttgaggttgt tgcgcatgcc gatacagaaa atatcctgtc agttgaactg cctcagttta
      421 ttgtttttga agtgacttac acggagaagg gactgaaggg ggataccgca accaatgccc
      481 agaaacaggc taccctggaa aatggttcgg ttataatggt gccccttttc atcaataacg
      541 gagataaaat caaggttgat actcacaacc gtgcctatat ggaacgggtc aagggctgat
      601 aataaccaca aaatgaagac acgcagcaac aggttacaaa tttccagatt caagcttggc
      661 gcattgcttg acataacaat ggcgatcaat gccaaccttc ccacagaaac ccttcttgca
      721 aaatatgaat ccatattaag ggatgatctt ggtatcggga aggttctcat cttcaaactt
      781 tcagacagct gggaatgcct tctgaatgcc ggtttccagg aagaacttga aagcctgaat
      841 gtggaggaaa agctgcttgg ctttgaagag atcacctttg tatcggcgga tatggatttt
      901 ccgggaatag acattattgt tccggtctat aacaataaca tacagattgc atttcttttc
      961 atcggagata ttgaggagga aggagaaggt atgagtccgg tactgaaaca tcttaatttc
     1021 attcagacaa tctcgaatat tattattgtg gccattgaga acatccgcct gcagaaagag
     1081 aggcttgccc aggaagctct taagaaggag ctggaactgg ctgcaaggat gcagaagatg
     1141 ctcattcctg acaaccgcca gcttccgaaa aggccggaaa tttctgttca tggcttttat
     1201 tatccgcatc atgaagtagg aggtgattat tatgattgca tcaggctttc cgatacaaaa
     1261 acaggattct gtatcgctga tgtttccggg aaaggcatgt ctgccgctat tcttatgtca
     1321 aacttccagg caagtttcag ggctctcttt tcagccgaat ccgatctcga ggaacttctt
     1381 tacaaactta attcaatagt aatcaagaat gccaaaggtg aaaagttcat cacctttttt
     1441 gttgcacgct atgatcatgt aacccgcagc cttgaataca taaacgcggc tcataccccg
     1501 ccggtaacct acaatacggc aacgggagag acagatttcc tcaatacaac atgtgtggga
     1561 gtaggtatgc tcgacagtat cccaagcatc aggacccggt caatcaatat cagcggccat
     1621 ttcaagatag tctgttatac cgacggactt tcagagctta aggatgattt tggatttgaa
     1681 acgggaacga gggaaatcat caatttcatt tcaaatgagc gcagcgtgga acaaaacatg
     1741 aaggacatgc tggatcatct cggtattccc gattccaacc cctatctttt tgatgatgtt
     1801 tccatccttg ctgcggatct gacctgaaga ttcaaggaaa cctcttcaga aactttcctt
     1861 tgcacctgca ccggatccgg gtaactataa aagcatccaa ggcaagggat tccgataagt
     1921 cccagcaaaa cagcagcttt aacctacttt tgtttcaggc agctctatga gcatcaggag
     1981 aaaagacttc agacaaacct cagcagaaat agttattctt acctgcaccg gatccgggca
     2041 actatataag catccaaggc aagagattcc gataagtccc ggcaaaacag cagctgtaac
     2101 ctgcttgtat ttccggcagc tctatgagca tcaggagaaa agacttcaga caaacctcag
     2161 caggaaatag ttcttcttcc ctttctgaac caccaggtac ttaccattca gaagctgatc
     2221 ctgtcccaca acaatatccg gatcgtcaat tttcaccttg ttaaggctta cacctcctcc
     2281 ctgtacaagt cttctgagct ctcccctgga actgaaaatt gccgaatgtt ccgcgcacag
     2341 ggccgtgaat cccgcaccgg ctgacaggat gccccggtca atatcaaatg agggaactcc
     2401 ttcaaagacg gaaaggaatg ttgtttcatt catctttctg agtgtttcgg tagttccttt
     2461 tccgaacagt atctgtgagg cctcgaccgc aagctgatag tcctcacgtg aatggaccag
     2521 gacggtgact tcctcggcga gccttttctg aagaagcctt tcatgaggtg acttgctgtg
     2581 ttccccgatc agttcttcaa tctcatcacg tcctaggaga gtaaatattt ttatgtatcg
     2641 tgctgcatcc tcatcggaaa cattgagcca gaactggtaa aaatggtatg gtgaggttct
     2701 ttccggatca agccacacat ttcccgattc cgttttcccg aatttggcac cgtcggattt
     2761 ggttatcagg ggacaggtca gtgcaaaggc ttcacctccg gttttccggc ggataagttc
     2821 ggttccggtc acaatatttc cccactggtc agatcccccc atctgcaatc tgcatccgat
     2881 ttcattgtaa agatgaagga agtcataacc ctgaacaagc tgataggaga attccgtaaa
     2941 ggacatcccc tctccttccg atcccagtct ctttttgacg gagtcctttg ccatcatata
     3001 attgacggtt atatgctttc ctatctgacg aataaattca aggaaggaaa attccttcat
     3061 ccagtcataa ttattgacca ttatggccat gttttttctt cctgagccaa aatcaagaaa
     3121 ttttgaaagc tgttttttta ttccttcctg gtttttacgc agggtcgttt catcgagaag
     3181 gtttctttcc tcagattttc ccgaaggatc tcctatcatt cccgtcgcac ctcccacaag
     3241 ggctacagga atatggccgg ccctctgaaa atgaaccagc agcataacct gaaccatatg
     3301 accaatatgc agggagtcag cggtcggatc aaaaccgata tagccttttg ctgtttcttt
     3361 ctcaagcatt tcctcagttc cgggtgttat atcatgcaac attccgcgcc atctgagttc
     3421 ttcaacaaaa ttcataagca gtaaaatttg agaaatgcaa atatagcaaa gtggattaaa
     3481 ttgacagctc tgagggatcc cgttcctttt ttggcttttc ttccgggggc ggtgtgaatc
     3541 tgtcaaagct ccgcttatag cttccctctc ccagaacagg gaaaatcatc ggagccacat
     3601 ctgaaatagg aatataaaac ctggagtttg aaatggtctt tttcggaaga aaggatttca
     3661 tggaatcgag ggcattgaga ataacaaaaa tcacactcat tataagtact gacttgagaa
     3721 atccgaataa catgcccagc aatctgttcg caaatcccag cgatgctgcc tccatgatcc
     3781 tgtcaacaat tattccgacg aaatgaataa ttatcattat taccccgaag gttataataa
     3841 aggccagtac cccgacaaac tgccctgtca tgtcaaacca ttcataaagt ttgactgctg
     3901 tgaaggatga gaatttaacc gctccccata ttccaagtat aagtgccgcc agtgcagcca
     3961 cttcctttat cagtccgtcg gtgaatcctc ttattatggc aactattata acaatgacta
     4021 taattaagtc aatccagttc attttgcgga aaattgaata tgtgaaagta tgaaacaatc
     4081 agcagttatc aaaatgcggt ccgccgtgag aggatgcccg ggaaaccggt tcgctagagc
     4141 ggatagagcc gggattttta aaatattctt taaagaaaat ctaaaaatat taacatttat
     4201 ttttctgata atcaatgttt ctagtggtta ttaacaaaac aggttctgta aatcttctat
     4261 ttcgcagcta aacattgctt aattttatat caacaggcag ttagcctgaa actaattgaa
     4321 agtcaatagt cgattaatta atttacctgt caagaaattt ctatgagtcc tgtctcattt
     4381 accggtgcca tacagcaggt tgcggaagaa tcccgtaatt accattcagc atccgggatt
     4441 gcagttctgg cacttttttg ctttctgacc ctgcaatgtc ctcttacaac agcacaaagt
     4501 ataattcgtt cttcattaag ttcaattgga acggctgttt cagaagaagg aattattttc
     4561 cggcaaacag tgggccaatc atcaaacact ttcattttta gaaatggagg aatatgtctt
     4621 cttcagggat ttcagcaacc aatatcaggt acatacttat tccggcctat ggatcctgtt
     4681 gatttcacac tctacccaaa tcctgccatg gataatacat ggctggttct gggagaagaa
     4741 atgaaatctt atactataac aatctataat ctgtcaggta ctcccctgtc ggttattact
     4801 ggctatgcct cacagtcagt ctggctcgat ctcaaaaatt atccggtagg catatacata
     4861 atcaaagtca caagtggtat aagaaccgga tctaaaagac tcatcctgaa tcattagata
     4921 aagttattaa catcatcaaa atcacgaaag atgaaaaaat atatgatttt aactgtgtta
     4981 gttattaatc ttctgcctct tgcaggtcag aataaaatag gtgtttcatt taatcagata
     5041 tattccacgt tcagattcaa aaattccgaa ggagagattg aggatctgaa atacaccatt
     5101 aaatatggtt atgacataat atggcaatat aaaatcctcg aagtcttttt tgttgaaggt
     5161 tccctgtcat ataactgcaa aggagcaagt tccactctcg gagtccaaaa acttgactgg
     5221 tcactccact atgcaaatgc aggagtaaat gccgcttata aaatgactat aggcaggctc
     5281 agtcctcatg gtggtgcagg attttactat ggcaggcttc ttaaggccga ccagttgata
     5341 ggatcggatt attacaacct gatggaaacg aattatatca ggaaaagtga tatcgggacc
     5401 aatatttttg cagggatcgc ttatgattat tccgaaaacg gatcagtatt tatcagattg
     5461 aacgaggctg tcggattact tcagcttgaa aagaatgact tgtcaagcca aagaatgttt
     5521 aaccgcacat tttcaatcca ggtcggatta ttcttcagca tacaataaac actaataaaa
     5581 gatgaaaaag agattatctc tcctgtttgc cattcttgga attgcaatat cgctatcagc
     5641 acagatagct gacggaatca cgtatcaggc tgtcgcactt gatgacaagg gaaaggaaat
     5701 tgcaggccat gatataaacg gcctcatcat tcatagcaag cagataagtg tcagattttc
     5761 aatattaagg aattcacctg acggagaatt gctttataag gaagttcatt caacttatac
     5821 ggacccgcac ggccttttta cgcttgtaat cggtcacggg gaggtttctc cggatggaat
     5881 gcatcagagg ctgactgaga tcaactgggg tgcaggcaga ctctttctca tggtggaaat
     5941 agatataaaa agcagaggta attttaaagt catgggtgtc cagcaaatga tggccgtacc
     6001 ttttgccttt cattccctta gcagtagtac ggttaaatac gatgatattc tgaatgtacc
     6061 tcagttatcc gctgtcgcca caacaggaaa ttattctgac ctgatcaata cgccttcggt
     6121 attcaacggt gattataacg aacttacaaa caaacctgtt attccggcaa aaaccagtga
     6181 gctcattaac gatgccggat ttattaataa ttattatgaa actgatccgg catggagagc
     6241 ttcattgcca tattattata ccaaagaaga cttaagaaat gcagattctt cgcagattca
     6301 tttccggaat ataatcaaca ctcccaaaac acttgccggt tatggaatta cggatgcaat
     6361 gagcaagtca gctcctgcca acagaataag cagctcagat attgcaaact ggaatacttc
     6421 cttcagctgg ggaaatcata ccggcttata ccggctttct tcctggcttc cttcctggaa
     6481 tgaaataagg gccaatccgt ttattattat atccccatca aataatcagc ttctgcgata
     6541 caattccact accggaaaat ggcagaactg gaccccggat tatctttccg actatactga
     6601 atcagatcct gtgtggactt cagcatcaat taattactat acgaaaacaa acctgcagac
     6661 aagtggaggt tctttggttc attttaacaa cattatcagc aggccgacaa caattaccgg
     6721 atacgggata actgatgcga tgagaacttc acatcccgca aacaatataa cagttgccaa
     6781 tattttgaac tggaatactg ctttcagctg gggaagccat atcgggttat atcgaccggt
     6841 cacctggctc ccttcctgga atgaccttac cgcaaatccc tttactataa ctgcaccttt
     6901 aaataaccag atcctgagat ataattcctc aaccgggaaa tggcagaact ggaccccgga
     6961 ttacctgaca ggttatactg aatcagatcc tttatggacc tcggtatctg tcaattatta
     7021 cactaaaatg aatctagaaa tgccaggatc gtcacggata aatttcgcta atatcatcaa
     7081 cagaccagga aatcttgccg gatacggtat aactgatgcg atggtcatca cacatcctgc
     7141 caatgccata accccgtcgg atattgggaa ctggagaaca gccttttcat ggggaaatca
     7201 tgctgctgca ggttatcagc cgtttatagt acccggagaa atacatcatt atctcagggg
     7261 agatatgacc tggcagccac tgactgctga agccgtgggc ctgggcaatg tagagaacat
     7321 ttcattgcac ggctggatgg gctccccgag cctgagtgta ctgggatttg tttcccaggg
     7381 tgaatggcat gccgggctta ttgatgccag cggcccggta atggcagatt taataattaa
     7441 aaaaggcgga tcgaacaggg agtttctgaa agccgacgga agcgtggatt caaataccta
     7501 tatgatacag acaacagaaa taacagatga attttctgct tcagacggcc agacaaactt
     7561 tactctcaca cgctatccat ctgctttcag cagaatcaaa atgtttgtaa acggcgtaag
     7621 gataagtaat actgcctatt ctgtttcagg tcaggcaatt acctatattt cagcaaataa
     7681 tggttcgtat gaacttgttg gaggtgacag aattcaattt gattattcct attgattatt
     7741 tcaaagattg atgaaactgc caattatttc cctggtattt attattatcc cgttacagat
     7801 ttctggccag gggattacaa agcatggccg gtcagctgcg gaagctcctg attttgtaaa
     7861 caggaacggg aaactaataa atatgccatc actaaacaga tatggtcagg agctattcaa
     7921 ggtaactgta acaacatttc cagtgacctc tgttaccagt aattcagcag aaagcggagg
     7981 gaggattgaa aatcccgaca atatagaaat tatcgcatgt ggtgtttgct ggagcacttc
     8041 tcccggccct gatatatcag attataaaac ttccgaaaat actgatgtaa ctgaatttac
     8101 aagtcatctt acccggctta ttccctcaac aacatattac ctgagagcat atgtaacaac
     8161 cattgcggga acggcctatg gtgaggaaat cagtttccgg acactgccca gaacagatcc
     8221 ctcagaatgc ctgatagcat ggtatccctt taacggaaat gcagatgaag caaccggttt
     8281 tggaattgac ggtattgtca acggagcagt attgtcggaa gacaggttcg gaaacactga
     8341 atcagcatat tactttaacg gtattgataa ttcaataact ataccgggtt acctgccggt
     8401 aacaaataca tttactttat cattctgggc ttacaatgaa aaagtaaaag gcagtgcaaa
     8461 tatcatcact gacggaagca gaaatatcgg cggcatggat tttctgctga acttcagggg
     8521 caatgaaata ggaataaggg ctgataaagg ctcacttctc aattatgaat actcaagtcc
     8581 agcggaatta cggaacctga accttttaaa cagatgggtc catgtagcct gggttatnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg ataccacttc ttcttcaatc
     8761 acggtggaaa gtaacattat cagtgacgga ggaaccgaag taacagagcg tggtgttgtt
     8821 tggagcactt ccttaaatcc cacaatccac gataataaaa cctctgatgg cagtggcaaa
     8881 ggatctttca ccagtcatat aacgggttta ttacccggga caacctacta cctcaaatca
     8941 tatgcaatca acaatgcagg cactttttat ggaaatgaat ggtcattccg gacacatgat
     9001 actttgtcga attgcgggac tgtcacagat attgatggca acatttataa tactgtcacg
     9061 ataggcaacc agtgctggat gaaggaaaat ctgaaaacaa caagatatag tgacggaaca
     9121 cccatttcat tggttgatga acagaatatc tggagtaagc tgtcaatcag cgataaagct
     9181 tattgctggt ataataatga tattaataat aaaaatccat atggcgcatt atacacatgg
     9241 gctgcagcaa tgaatggagc tgattccagt acttcaattc caagcggtat ccagggtgtt
     9301 tgtccgacag gttggcattt accaagctgg agtgagtggg gtatccttat acaatatctt
     9361 gatccgcaaa ttgttgatcc attatatctt cgtgggagta gtatcgccgg tggtaaaatg
     9421 aaagaaagcg gattcacata ttgggattat cccaacaccg gagcgacaaa tgaaagcgga
     9481 ttttcagggc ttcccggtgg cggcagatca gaggacggag aatttctttt tcttggtaat
     9541 tcttcttatt ggtggagtac atcacaattc ggaagcggta attataccag agttcaaaat
     9601 ctcaattaca acaacagttc taatcaaatg actagcaccc cctggaaaag cgggatatca
     9661 gttcggtgta taaaaaatgt tgatcagctt atacaattac ctgttatcac tacaacacag
     9721 atttcccata tatctgatac ttctgcaaca agtggaggaa aaattattaa taacggagga
     9781 gatgaaatta ttgcccgtgg catctgctgg agtttgtcaa ctaatccaac aacaacaaat
     9841 aacaagacaa acagtggtac cggaaccggt ttgtttattg atcacattgg tccattaacg
     9901 ccaggaacaa catattatgt acgtgcatat gcaactaata gtgcagggac agcctatggt
     9961 aatgaagaag tatttgtaac ctgtgattac atacctcctg ttgcttttaa ttcaaacctc
    10021 gcttacggag tgataacaga tattgaaggt aacttttata aaaccataaa gattggtaat
    10081 caggaatgga tggctgaaaa tttaaggact acaaaatata acaatggtga atcaatacct
    10141 gttataacag ataatgacac gtggcaaagc ctgtcagaag gtgctctttg ttggttaaat
    10201 gataatatct gttataagaa tacatatggt ggtatctata actggtatgc tgccaataat
    10261 aatctatgtc cggttggttg gcaccttcca acaaatgaag aatggtcagt gatgaataga
    10321 gcacttggag gaaatgaaat tgcgggcgga aaacttaagg aacaaggatt tagtcactgg
    10381 agcgaaccaa atacaggtgc tactaatgaa agtggatttt cagctttgcc aaccggagga
    10441 tttcgtaatg gagctttatt tgttacaggt gagtttgcca gcttttggac ctcaactgaa
    10501 tatcctgaca atcaggcatt ttacagaagc ctgcaacacc aatatagcat agagggttat
    10561 agctggggtt ataagtcaaa tggcgccaat gtaagatgcc ttaaagggcc tgctcctcca
    10621 aaaccctctg taaccacact ttgggcaaga aatatcatgt ctgataaagc agaaatagtt
    10681 tcagtcatca gcagtgtttt gcctgttaat tcaagaggag tttgctggaa cacttcaccg
    10741 gctccggagg tactggataa caaaacaaca aacgatggcg gcacgggatc attcacaagt
    10801 ctgctaaccg gactggaatc caataccaca tattatgtca gggcttactc gattaatgat
    10861 gatggtatta cttacggaaa cgaaataacc ttcagcactg tagaagattt gccaatcggg
    10921 ggtcttgcgg cttactggcc ttttaacggc aatgcaaatg atgaatcggg taataataat
    10981 aatggcactg tatacggcgc cacccttgtc gcagacagat tcggaaatcc tgagaaagca
    11041 tactatttta atggcatcag caacaaaata tatattggaa attcactgat agttcagggg
    11101 catattaccg ttgcatgctg gattaaaagt accgatattg gtaatcagat aatttcatcg
    11161 ggaagcagaa attcctacag aataggaaaa acaaaggatg gggttgatgc ggctgtctat
    11221 ctgactgata gctccgatgg ttatggactt gatgtccatg cggttaccga ttgcagtgac
    11281 tggacgcatg tggctattgt gtataatgga gtcagccttg acctatatgt taacgggcaa
    11341 ttaatggact cagcctatgg acccggaaat gttttttgta atcagccagt ttatctgaat
    11401 tttggcacat atcagtggtt atctacatat tacggttttt atcagggata tcttgatgat
    11461 gtgtatatgt tcaacagggc actttcaaat tcagaaattg aggcattgta tcatgaaggt
    11521 ggctggtaaa tgatgctgaa gaaaaaagga aacagagtca ggcaaaacaa ttatagtact
    11581 atgcaataca aatcccaaac aattatcaaa atgaagaatc tttttaaatc agtccttatt
    11641 gcattgcttg tttattctat gttatcatgc agcagtgata aggaaaagac agaatcattc
    11701 accacaagaa gtgttgaaga aaacaaagct actattgagt ctgccggaat tgacctgatc
    11761 ggggttctta accgtatgag atcaactcaa acagttgaag ctatcggtaa tatgatacaa
    11821 ctaacaggat caggcaaatc aaaaggaatc ggggatttta ccggaacagg tctctattcc
    11881 gtatttcgct cgatacatgg atgtgccgca cgaaccctgc aggtaaacga tgtattcgag
    11941 tccataaaag gctgtgctga cgaagacccg gaaagtatac aggaattctg ggacagaaac
    12001 gtgggaacct atacatggaa ttcctctgcc agcgactggg atataagtcc gggtggggat
    12061 aaattcatta taaagttccc ttcttccgga aattcaaaaa tgaataatgc cacgtttaca
    12121 atatacaatt atgaaggtgt aatcgtggaa aatccatttg atgaagaata tacaggagat
    12181 tttcctgctt cattacaagc cgatctgaaa atcggaagcc agacactgct ccggtttgta
    12241 ttcggtgcaa gctataacgc agacggaata ccaaatgcaa ttgcatgcga tctggatttt
    12301 gaaggattta aattcgaaat tgatgtggca aacgatacca gggtaatatc tgcgtcttac
    12361 aagttcatgg aagggaaagc agtaataatg aaattatccg ctgttgtcaa tggacttttt
    12421 accgctgata accttgaact caatactgtt catcattctg aatcccattc ttatgtatgt
    12481 gactttgtct ataatccgga aacaaaggaa tatgttgagg tttattgtga atacaccaat
    12541 gaatgggaga aaattgaatt tgaagagatt gcacattctg caaatgctga gttccagtta
    12601 atgaatctgg ctgtccgtgg tgatattgat ataaaaaaac tggttgacaa aatcaggatt
    12661 attgaggatc agtatgacac cgaacaaatt gaagaagaag aatatttcaa cagcatatc
//
LOCUS       DAOSPX010000061        12666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_4992_length_12666_cov_4.605027, whole genome shotgun sequence.
ACCESSION   DAOSPX010000061 DAOSPX010000000
VERSION     DAOSPX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12666
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4992_length_12666_cov_4.605027"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLZ75_04700"
     CDS             <1..590
                     /locus_tag="PLZ75_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71732.1"
                     /translation="DAYGFGHFIESLSNGRIAVSHGGQGTGWMTHFHSVPSTGDGIVI
                     LTNSQRSWLLISHLLTDWAGWNGFSGVGMSRILLGEKIIRTLIVLLLSLLLWQAGRLI
                     IAVFSGRRRFRLLSGTSGLKSYVYFGCFVIIASCLILAVTRKYLFISSLYPSSSRWLG
                     YSLAFGSFLFLMSSFFPSITKSKGGCVHFPCNTAE"
     gene            complement(591..2624)
                     /locus_tag="PLZ75_04705"
     CDS             complement(591..2624)
                     /locus_tag="PLZ75_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS71733.1"
                     /translation="MNKFYLIGFLTFLPRIISGNSPDSISVHDTLLSRTLSEVVITAN
                     RFGSTGINTPEAIKIAGEPAIMKNQLRTAPEALSITPGVFVQKTNHGGGSPFLRGLTG
                     NQTLLLIDGIRLSNSTTRYGPNQYFNTIDVFSIEKMEVLRGSGSVQYGSDAIGGTIQA
                     FSHQLRTTDEALWGSSLLTRFGTHGMEQSIRGGITFSNSRIALRSGISLRNFGDIVGG
                     DTTGKQSPTGYREFDYDLKGKIILSEKTSATILYQDVNQMNVPVYHKVALENYAINKM
                     DPQKRKLAYLRMDHDMNLGIFKSVSVTASFQNSREIREMQKNGAATLRIENDKVRTLG
                     FSAESVVETAEIWTGIIGFEVYNDLVNSTRTDISAGNAVGKRGLYPDGSSMVSMAGFT
                     VNSFDLGKWIITAGTRFNTYEIKVNDEALGLTRLTPSALVGNLALLRKLSATSNLFAS
                     LNTGFRAPSIDDLGTLGIVDFRYETPNLDLKPESSVQYQIGYKYLDRKVRGEIYIYRN
                     QLYNLITRSRVGEQVIEGYPVYQKENSGKSFIQGIETAWDYDFSQAWSFHGDITYTYG
                     QNLTKNEPVRRIPPLYGRIAAEYSAGNWLLNIEWLAAAKQSRLAQGDKDDNRIPEGGT
                     PGWNIFNINGSYSWKFITIDLSLRNILNTDYRYHGSGVNGYGRSLFLTVGIDI"
     gene            2857..3789
                     /locus_tag="PLZ75_04710"
     CDS             2857..3789
                     /locus_tag="PLZ75_04710"
                     /inference="COORDINATES: protein motif:HMM:NF017953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOS71734.1"
                     /translation="MAYPIERKLVIAIASSALFDLSESDKVFREKGVEGYRKFQEVNI
                     DKTLDKGVAFPFIRRFLNLNSVFPEEEPVEVVLLSRNSPETGMRVFRSIRDYGLNITR
                     AAFFSGKSPYEYLPGFNASLFLSANSEDVNNAIRAGFPAGRVIETRISDDDSDNELRI
                     AFDFDGVIADDAAEVVYKSTRDLNKFHETETQLSLVPHNPGPLQDLFKKISFFQKLET
                     REIRNNPDYERILKISIVTARSAPSHERMINTLKNWGVSVDETFFLGGIEKKRILEIM
                     KPHMYFDDQSVHLEHIDTIPLVHIPFGIANIDSL"
     gene            4120..4614
                     /locus_tag="PLZ75_04715"
     CDS             4120..4614
                     /locus_tag="PLZ75_04715"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOS71735.1"
                     /translation="MKRRNVLKNLAAVPLAGVAGHEMSVFDKPGKGGEWLPKKEQIAP
                     VYKGSAPVSLCIRSGHLVFLSGIGGWYEHRRAEPGDIKVQIASALTDMKNQLEQAGTS
                     MANVLKVHMTVVDPNNNIGPLNEVYQTFFPDPKPARSYTGCTADLMGRDGILVQIDCV
                     AYID"
     gene            4883..5890
                     /locus_tag="PLZ75_04720"
     CDS             4883..5890
                     /locus_tag="PLZ75_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71736.1"
                     /translation="MKQLAVILVSFLILVRVYADIAPNPIIIKGIFTPDSCSIRMTRE
                     YVSADLYNDSARVECVFELLNTGDSITIQTGFPVMNFQYWSMGEYSEDDKTHFRIFVN
                     GRMLTENEIGVPAEMDSIYRAYMEIYHYEREYRRKTDSIYSANMVTVKRNGNLNYPSD
                     RSYQMTSSAMRMLYEWRETKPFFSSDLWKQFDNQMSKGNFPWYVWNIHFGKNEKKQVK
                     VVYSLPSGQGYGANYRYFKYILETGAGWYGTIGEANIELKFHDIEFGTLEEILPSGYL
                     ADRESKTIEWAFCNFEPAKDDDIYVRYYNKKERESWKRYQRKRKRAIIFRQLNPVNWF
                     G"
     gene            6226..6687
                     /locus_tag="PLZ75_04725"
     CDS             6226..6687
                     /locus_tag="PLZ75_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOS71737.1"
                     /translation="MSFEKVKNYFESLSLGGRIKTFEDSSATVEMAARAIGCEIDQIA
                     KTLSFLVSDLPVLIVAAGNVRIDNRKFKAFFHQKPKMIPGELVNEYTGYDSGGVCPFE
                     VKPDVKIYLDVSLKNNEIIYPGAGSGNSIVELSAGELEKYTGYEQWVDVCK"
     gene            6762..6974
                     /locus_tag="PLZ75_04730"
                     /pseudo
     CDS             6762..6974
                     /locus_tag="PLZ75_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            7139..7810
                     /locus_tag="PLZ75_04735"
     CDS             7139..7810
                     /locus_tag="PLZ75_04735"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOS71738.1"
                     /translation="MKNNVLFRKIQTVLIMFLQAVILFATAGTLKWKWAWMFIASQVA
                     ILIVNALVLPRELIEERGRIRKDVKKWDRILNRINIIPFSGIFILSGFDYRFHWTGSF
                     GLEIHIAGLILILLGSMIFTWSMISNKFFSTMVSIQDDRGHSTATGGPYRYIRHPGYT
                     GFIIMTIATPVALGSFYALTMAFLVSVIFIVRTIFEDKTLISELPGYLEYSEKVKYKL
                     IPYIW"
     gene            complement(7973..10396)
                     /locus_tag="PLZ75_04740"
     CDS             complement(7973..10396)
                     /locus_tag="PLZ75_04740"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS71739.1"
                     /translation="MKSRRIIITLVILSAITQVLVAQTSISGRVLSREDRQPLISSTV
                     SVVKAGSGELVTGTLTNEEGRFNVSGLATGEYDFIISYLGYKTEKRKVLSGSLNKFLD
                     IGNILLEPSGFELSEVKIEGQKLTTSATLDMKSYSADNFVTGSAGSVLDILKTLPGIV
                     IDQESHIVLRGSDKVAILIDGKPSSLTGFGSQKGLENIPASQIESIEIINNPSAKYDA
                     AGMAGIVNIKYKKSESVGLSGDAGFTPGIGMITKRKADLPTGMSSYSNNRKLTPSLNL
                     NYKTEKLNIYWQSYLLNQRRLPNNEFTTRFYDNGNITESQVAENRSQNHYNFKLGVDW
                     NPAPDQTITLFGLYDYEWHIDTTRVWYFGNTDYNNPLRKWGFNESEGTGFTNLTLQHK
                     YRFPQSGHELNSQLLFTKGWEDETYNLKQDGPLPYPVIKTDKTRVLAPEYIYLISSDY
                     VRPLAFGRLEAGISGRLRHMPITYTVTKDPANTALIYNFGDWSKWNESLAGAYLNLVA
                     ELAKLNIEAGLRGEYTAISYKFAPNPYFSEDQYNYFSLFPNTRFTFIINKSNKISLFY
                     NRRIDRPGEDILRIFPKYDDPELLKIGNPSLRPQFTQNVEIAYRCLWQTGTFYTSLYH
                     KIIDDYYTRVYIQDPYHTEISIKAYDNLGKATNTGIELGVDQNIVKAWKVTASANLYS
                     NTIFEHEGQIMFPVEQSYIIAKRTDMPVYLKMTNQLTTRNDFIFELTAQYFSEKNIGQ
                     GKELSRGGLDFGMKKKFFENKLEINLTASDIFNTMGLRQKIEAEGFNVEYQNFYETQI
                     FLLALKYRF"
     gene            10650..10841
                     /locus_tag="PLZ75_04745"
     CDS             10650..10841
                     /locus_tag="PLZ75_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71740.1"
                     /translation="MRKFRQIIIIGSVILLIVFLAMFDYSNPWTRSNHAVNVGIVAVC
                     LNIIAMILSNRHDEKAGKK"
     gene            11046..11903
                     /locus_tag="PLZ75_04750"
     CDS             11046..11903
                     /locus_tag="PLZ75_04750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOS71741.1"
                     /translation="MREKPQLLPYWTLWVPGATYFYQGRIIQGSVFSALELGGIYTGI
                     KYDKNLRGNSSSPYYNYPLLMGLQAFQTEKLTMFSNQLEVFKYNYPDFRYDCLSEKDL
                     YLAPFKIKNIMTPITGGMVLLAGVFLGIEKFNETKRFTDIERMYFLNRYISRDQGLAV
                     FGTVSLAAAWSAGIGEEYVFRNWMMPLLDYKYGQKKGLIISSAIFGGVHFTNVLFAEK
                     PDYKAALLQVGEAAIAGYFLGRDVQKRGYNIGPAVAAHMWYDFTLMLGSFLINPQNNF
                     LGVNLKFRI"
     gene            complement(11974..>12666)
                     /locus_tag="PLZ75_04755"
     CDS             complement(11974..>12666)
                     /locus_tag="PLZ75_04755"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOS71742.1"
                     /translation="EKRIPGGACEHSPGSGTLYLERPGASTFARSWVPSIGGFQGMLV
                     THDEVMSIADYLSVRNEDSFLYRPTVMFVYHPCDDAMLSALELEGSGWVLQASARRLG
                     PDIVEGTDELGVLLAGHERNAYWYGSQLGIEEARRHVDYANATTIQVTAGAIAAILWA
                     IKNPRRGIVEPEDLDFEDCLETAIPYLGRMVSEFTEWTPLDGRGKYFPEKLDVQSPWQ
                     LQNIRSRQYYGE"
BASE COUNT         3555 a         2539 c         2780 g         3792 t
ORIGIN      
        1 ttgatgcata tggattcggg cactttattg aaagtctttc aaacggcagg attgctgttt
       61 cgcatggggg tcaggggacg ggctggatga ctcatttcca ttcggtccca tcaacgggag
      121 acggaattgt gattctgacc aacagccaga gaagctggct gctgatctca cacctgctga
      181 ccgactgggc cggatggaac ggattctcgg gtgttggaat gagcaggata cttcttggcg
      241 aaaaaataat ccggaccttg atagtattgt tactgagcct cttattatgg caagccgggc
      301 ggcttattat tgctgtattt tccggccggc gccgtttcag gctgctttcc ggaacatccg
      361 gactaaagag ttatgtatat ttcgggtgct ttgtaattat tgcctcctgt cttatcctgg
      421 ctgttaccag aaagtatttg ttcatctcct ccctctaccc gtcatcttcc aggtggcttg
      481 gttattccct ggctttcggc tcttttcttt ttctgatgtc ttcttttttt ccttccataa
      541 caaaaagtaa ggggggatgt gtacatttcc cctgtaatac agcagagtaa tcagatatct
      601 atccccaccg taaggaagag cgatcttcca taaccgttaa cgcctgaacc atggtaacgg
      661 taatcggtat tcagaatatt ccttaagctc agatcaattg taataaactt ccagctgtaa
      721 ctgccgttaa tattaaaaat attccatccg ggagtgccac cttcgggtat gcggttatcg
      781 tccttgtcac cctgtgcaag acggctttgc tttgctgccg caagccattc gatattcaaa
      841 agccagttac cggcgctgta ttctgccgca atccttccgt acaagggagg tatccttctc
      901 accggttcat tcttcgtcag gttctgtcca taggtatatg ttatgtcgcc atgaaaactc
      961 cacgcctgac tgaaatcata atcccaggct gtctcaatac cctgaataaa tgatttgccg
     1021 ctgttttcct tctgataaac agggtatccc tcaataacct gttctccaac cctgcttctg
     1081 gttatgagat tgtacaattg attacggtaa atataaattt cacctctcac tttccggtca
     1141 aggtatttgt acccgatctg gtactggaca gagctttccg gtttaaggtc cagattcggg
     1201 gtttcatatc tgaaatcgac tatgccaagt gtgcccaggt catcaatact aggtgccctg
     1261 aaacctgtgt tcagtgatgc aaaaagattc gatgtagcgc ttaattttct gagaagagcc
     1321 agattcccca ccagtgccga gggagtcagt cttgttaagc caagggcttc atcattcacc
     1381 tttatttcat aggtattgaa tctggtaccg gcagtgatta tccattttcc aaggtcaaat
     1441 gaatttaccg tgaatcctgc catactgacc atcgaggatc cgtcaggata taatccccgt
     1501 ttccccactg cattacctgc ggaaatatct gttcttgtac tgttaacaag atcattataa
     1561 acctcaaagc ctatgattcc ggtccatatt tcagctgtct caaccactga ttccgcagaa
     1621 aaccccaatg tacggacctt gtcattttca attcgcagtg ttgctgcacc attcttctgc
     1681 atttccctga tctccctgct gttctggaag gatgcagtta ccgatactga tttgaatatc
     1741 ccaaggttca tatcatgatc cattcttaaa taggcaagtt ttcttttttg agggtccatt
     1801 ttatttatgg cataattctc aagtgccacc ttgtgatata ccgggacatt catctggttt
     1861 acatcctgat aaaggattgt tgcgcttgtc ttttccgaga gtattatttt tcctttgaga
     1921 tcatagtcaa attccctgta acctgtcggt gattgtttcc ctgttgtatc gcctccgaca
     1981 atatctccaa agttacgcaa gctgatacct gaccgcaatg caatcctgct gttactaaag
     2041 gttatccctc ccctgatact ctgttccatc ccgtgggtgc caaatcttgt tagcagtgat
     2101 gaaccccaca gtgcttcatc tgttgttctg agctggtggc tgaaagcctg gatagtacca
     2161 cctatggcat cagatccgta ctgaacggat ccgcttcctc tgagtacctc cattttctct
     2221 atgctgaaaa catcgatagt gttgaaatac tgattgggtc cgtaacgcgt ggttgaatta
     2281 ctcagcctta taccgtcaat aagaagaagt gtctggttac cggtaagtcc tcgtaaaaaa
     2341 ggggagcctc cgccgtgatt cgttttctga acaaacactc cgggggtgat acttaacgct
     2401 tcaggagccg tccgcagctg atttttcatt attgcaggtt cacctgcaat ttttatggct
     2461 tccggagtat tgatacctgt tgatccgaac ctgttcgccg taataacgac ctccgacaga
     2521 gttctggaaa gaagagtgtc gtgaacagaa attgaatcgg gtgaatttcc gctgataatc
     2581 cgtggaagaa atgttaaaaa tccaatcagg taaaatttgt tcataataat aaatgagtca
     2641 agttaagaac aaatttaatt taaagtttgt gttgccggca tgtttgcatg aaaaatgctt
     2701 ccggaggtat aatatagccg gcaaatcagg aaaaatcagc ttacattttt cagccggcct
     2761 catgatttat aataaaaatc cgggattccc atcttcatcg ttgtccttct ttaccgcata
     2821 ataaactaac tttgaaaaac cattaaacca tggatcatgg catatccgat agaaagaaaa
     2881 cttgttattg caattgcgtc aagtgccctt tttgatttgt cggaatcaga taaggttttc
     2941 agggaaaagg gagttgaagg gtatcgtaaa tttcaggaag tgaatatcga caagactctg
     3001 gacaaagggg ttgcgtttcc ttttatccga aggtttctta atttgaattc tgttttcccg
     3061 gaagaagagc cggtggaagt tgtcctcctg tcgaggaaca gccctgaaac gggcatgagg
     3121 gttttccgtt cgatcaggga ctacggactg aatattacca gggctgcatt tttttccgga
     3181 aagtcgccat acgaatattt accgggtttt aatgcttcac tgtttctttc tgcaaattct
     3241 gaagatgtta ataatgcaat acgtgcggga tttccggcag gaagggtgat tgaaacaagg
     3301 atatcggacg atgacagtga taatgaactg aggatagctt ttgactttga cggtgtgatt
     3361 gctgatgatg ccgctgaagt tgtttacaaa tcaacaaggg atctgaataa atttcatgaa
     3421 actgaaacgc aactgtctct ggttcctcac aatcccggac cattacagga tttattcaaa
     3481 aagatttcat ttttccagaa gcttgaaacc cgggaaatca ggaacaatcc ggactatgag
     3541 cggatattga aaatttcaat tgtgactgcc agaagcgccc cttctcatga aagaatgatt
     3601 aataccctta aaaactgggg ggtatctgtt gatgaaacat tttttttggg aggcattgaa
     3661 aaaaaacgga ttcttgaaat aatgaaacct catatgtatt tcgatgatca gtcagttcat
     3721 ctggaacata ttgatacaat cccgcttgtt catattccgt ttggaattgc aaacattgat
     3781 tccctgtaaa taaggggact ccggttaaaa tatatatccg cgccgttaga gtaaatattt
     3841 atccttactg ttggattata tatatccttt tttgttggat taaatttgta tcctttcttt
     3901 ttttaagagt ctggataaaa tttatccatg tcctccgtaa aacctgataa aacacaaaac
     3961 ccggccggtt ttgcagtcag agaatgtttt gtatcttccc ttgcaaaatt ttgtacgtat
     4021 gaaacccgta tgactgacca gaaaacaaac acgaatgaat attatcgcgt tcagtcatgt
     4081 atggttccat cagatcttta aaaaataaat tatatacgga tgaaaagaag aaatgtgttg
     4141 aagaatcttg cagcagtacc gcttgccggc gttgcaggtc atgaaatgtc agtttttgat
     4201 aagcccggaa agggtggcga atggctgccg aaaaaggaac aaatagctcc tgtttacaaa
     4261 ggttcggcac cggtgagtct ttgtatccgt tcaggacatc ttgtatttct cagtggcata
     4321 ggaggatggt atgaacatcg cagggctgag cccggggata tcaaggtaca gatcgccagt
     4381 gcactcacag atatgaaaaa tcagcttgaa caggcaggca catcaatggc gaatgttctg
     4441 aaggtacata tgacagtggt ggaccccaac aataatatcg ggccgctaaa cgaagtatat
     4501 cagacatttt ttcccgatcc caagcctgca aggtcataca cgggatgcac ggccgacctc
     4561 atgggacgtg acggaattct tgtccagatc gattgtgtgg catatattga ctaagccggg
     4621 agagaaggtg ccggacaaca gaagaaacag gcgggttgcc ggatagaaac ataccagagg
     4681 gggccgctga atgcatcctg agcccgcagg ggcgggatca gccgctgttc aggcatcatg
     4741 tatcgctatt tatgttttct gtgccgcaag cttgtaacgt tatttctgcc tcctgcccgt
     4801 tcgcacaatg agaggaaaac atttcagaag cggaaggaca acgtgcgata atcaatgaat
     4861 tcatttttgt aaaagatgct atatgaagca attagcagta attctggttt catttctgat
     4921 tcttgtaaga gtttatgcag atatagctcc caatccgata atcataaaag ggatattcac
     4981 acctgacagt tgcagtatcc ggatgaccag ggaatatgtt tctgctgatc tttataatga
     5041 ttctgcaagg gtagaatgtg ttttcgagct tctgaacaca ggagacagta ttacaattca
     5101 gaccgggttt ccggtaatga atttccagta ctggtctatg ggagaatatt ccgaagatga
     5161 caaaacacat ttcaggatct ttgtcaacgg cagaatgtta acagaaaacg agataggggt
     5221 gccagcggaa atggacagca tctacagggc ttatatggaa atatatcact atgagagaga
     5281 ataccggagg aaaaccgaca gtatatattc tgcgaatatg gttacggtaa aaaggaacgg
     5341 taacctgaat tacccgtcag accggtcata tcaaatgaca agttcagcaa tgagaatgtt
     5401 atatgaatgg agagaaacca agccattttt tagttctgat ctgtggaaac agtttgacaa
     5461 tcagatgagt aaggggaatt ttccatggta tgtctggaat atacacttcg gtaaaaatga
     5521 aaaaaaacag gtcaaagtgg tttactcact gccatcgggg cagggatatg gtgcaaatta
     5581 cagatacttc aaatatatcc ttgaaacagg agccggatgg tatggaacta tcggggaggc
     5641 aaacattgag ttaaagtttc atgatataga atttgggacc ctggaggaga ttctcccttc
     5701 aggatatctg gcagacaggg agagtaaaac aatagaatgg gcattctgca attttgaacc
     5761 agccaaagat gatgatattt atgtaagata ctacaacaaa aaggaaaggg aaagctggaa
     5821 acgttatcag agaaaaagga aacgagcaat aatattccgg cagttaaatc ctgtaaactg
     5881 gttcggataa tctttggagt gggcattctg aagcacctgt attttcgttg ccggataaaa
     5941 tcatttattt cagcagaaaa ccgcagaaaa ctcttactgc ttctgtaaat ccgggatatc
     6001 tgcagggtat ggattcggtt gtttatcaga acttccggtt ttacctggcg tttttttcgg
     6061 atccggtaac actatttgcg gagctgcaaa acagaaaaga gaagagaatt tccaaattcc
     6121 gggagaacag acagagggcc gtgcagatcg gaatgaccgg gaagaccaag aagaccgggg
     6181 aaaattattt gaagtatatt atgaaccgaa tattaaaaaa gaacgatgtc atttgagaaa
     6241 gtaaagaatt attttgaaag tctgagtttg ggtggccgga ttaaaacgtt tgaagattcc
     6301 agtgctacag tggaaatggc agccagggcc attggctgtg aaatagatca gattgcaaaa
     6361 accctgtctt ttctggtcag tgatttgccc gtattgattg ttgctgccgg aaatgtaagg
     6421 attgataaca ggaagtttaa ggcctttttt catcaaaaac cgaaaatgat tccgggtgag
     6481 ctggtaaatg aatacacagg ttatgattcg ggaggtgtct gtccgtttga agtcaagccg
     6541 gatgtgaaaa tctatcttga tgtttcctta aaaaacaatg aaataattta tcccggtgcc
     6601 ggaagcggca acagcattgt agaactgtcg gccggggagc ttgaaaagta cacaggctat
     6661 gaacagtggg tggatgtctg taaataaggg agcttgttgg ataccggtaa aaaacctgaa
     6721 aaatattatg aaacgcgaaa aaggagaaaa gccgaaacaa tggtacgaaa tattgtttga
     6781 aaattattga tatcggatgc ggaacaggcc gccattcaat tgaattggcc aaaagaggct
     6841 attctgtcac tggtgttgat ttatccggat ccatgctgga aagagcaagg gaaaaggccg
     6901 ggcagaatga tgtgcagata gatttcttac agcttgatgc acgtaattgc agaaaaatga
     6961 actctgttgc ctgacaataa agacttgccc cggtgagaaa tctctcttcc cggaaaagcc
     7021 atcctgttaa acccggacaa ttagtatgag taagatattt caaataaaac atctctgatt
     7081 catccttact gagctgatta ttctgtattt tgctgtttaa atattaaaat ccgcggattt
     7141 gaaaaacaac gttctgttca ggaagattca gaccgttctc atcatgtttc tgcaggctgt
     7201 gatactattt gcaactgccg gtacactgaa gtggaaatgg gcctggatgt ttattgcctc
     7261 ccaggtggca atactgattg tcaatgcgct ggtactgcca agggaactca ttgaggaaag
     7321 aggcaggata cggaaggatg taaagaaatg ggacaggatt ctgaacagga taaacattat
     7381 tccattttcc ggtattttca ttctttcagg atttgattac aggtttcact ggacaggctc
     7441 gtttggctta gaaattcata ttgcaggact gatactgatt ctcctgggtt cgatgatctt
     7501 tacctggtca atgatttcaa acaaattctt ttcaaccatg gtcagtattc aggatgaccg
     7561 agggcacagt acagctaccg gggggccgta caggtatata cggcatccgg gatatacggg
     7621 ttttatcatt atgacaattg caacacctgt ggcactggga tcattttatg cgttgactat
     7681 ggcatttctg gtttcggtca tatttattgt ccggacgata tttgaagaca aaacacttat
     7741 ttcggaactc ccgggctacc tggagtattc tgaaaaggta aaatataagc tgatcccata
     7801 tatctggtaa atgaccttgt caggtttgcg atggaaaatg ctttgttgtt ttccggctca
     7861 ccctgatttt tttgccaaat gactggttca gttttacgac gggaaatact ttgttattcc
     7921 gcaacaccca ttattttctg tactaaaagg ccgggatctg tttttgattt tttcagaacc
     7981 tgtattttaa tgctaataag aaaatctggg tttcataaaa attctgatat tccacgttaa
     8041 aaccctccgc ttcaattttc tgacgcaaac ccatggtgtt gaaaatatca gaagccgtaa
     8101 gatttatttc aagtttattt tcaaagaatt tctttttcat tccaaaatct aatccccctc
     8161 gtgacagctc ctttccctgt ccaatatttt tctcactaaa atactgagcg gtcaattcaa
     8221 aaataaaatc attacgagtg gtcaactggt ttgtcatttt gagataaacg ggcatatcgg
     8281 tacgtttggc gattatatag gactgctcca caggaaacat gatctgacct tcatgctcaa
     8341 atatcgtgtt actgtataag ttcgcgcttg ccgtaacttt ccaggctttc actatatttt
     8401 gatcaacgcc tagttcaata cctgtatttg tagcttttcc aaggttgtca taagccttta
     8461 tggatatctc agtatggtag gggtcctgaa tgtaaacacg tgtatagtag tcatctatga
     8521 ttttatgata taatgaagta taaaaggtac cggtttgcca caaacacctg tatgcaatct
     8581 ccacattctg tgtgaactgt ggtctcaatg aaggattgcc gattttaagc agctccggat
     8641 cgtcatattt agggaaaatt ctgagtatat cttctccggg gcgatcaatg cgacgattat
     8701 aaaacagaga gatcttattc gatttgttta tgatgaaagt aaaacgtgta ttcggaaaca
     8761 gactgaaata attatactga tcctcactaa aataaggatt gggagcaaat ttatagctga
     8821 ttgctgtata ttctccgcgt aatcctgcct cgatgttgag ttttgccagt tcagccacca
     8881 gatttaaata agctccggcc aggctttcat tccattttga ccagtcacca aaattataaa
     8941 tcaacgcggt atttgccgga tcttttgtta cggtgtaagt tatgggcata tgccgcaatc
     9001 tgcctgatat cccggcttcc agtcttccaa aagcaagtgg gcgaacataa tcggatgaaa
     9061 tcaggtatat gtattcgggt gcaagaacac gagttttatc tgttttaata acaggataag
     9121 gaagtggtcc atcttgtttg agattatatg tttcatcttc ccatcctttt gtaaaaagca
     9181 gttgtgaatt caattcatgt ccagattgtg gaaacctgta tttgtgctga agtgtcagat
     9241 tggtgaatcc cgtgccttca ctttcattaa atccccactt tctcaatgga ttgttatagt
     9301 ctgtattgcc aaaataccat accctcgtgg tatcaatatg ccattcataa tcatagagcc
     9361 cgaaaagagt tattgtctgg tctggagcag gattccaatc tactcccagt ttgaagttat
     9421 aatgattttg tgaccggttt tcggcaacct gactttctgt aatattcccg ttatcgtaga
     9481 agcgggttgt gaattcatta ttcggtaatc tgcgctgatt taacaggtaa gactgccagt
     9541 aaatattgag tttttctgtt ttataattca ggttcaatga aggagttagt ttccggttat
     9601 tcgaatagct tgacattccg gttggaagat cagccttcct tttagttatc atgccgatac
     9661 ctggtgtaaa gccggcatca ccacttaaac caacggattc tgatttctta tatttgatat
     9721 ttacaatacc cgccataccg gcagcgtcat attttgctga aggattgttg ataatttcaa
     9781 tactctcaat ctgagatgcc ggaatgtttt ccagaccttt ctgacttccg aatcctgtaa
     9841 gactggaagg tttcccgtcg atcagaattg ctactttgtc acttccgcga agcacaatat
     9901 gactctcctg atcgatgact atccctggca gagtcttcaa aatatccaga acagagcctg
     9961 cggaaccggt aacaaagtta tcggctgaat aacttttcat atcaagcgta gctgaggttg
    10021 taagcttttg cccttcaatc tttacttcag aaagttcaaa accagatggc tccaatagaa
    10081 tattacctat gtcgaggaat ttgttaagtg aacctgaaag caccttcctt ttttcagttt
    10141 tataacctaa ataggaaatg ataaaatcat attctcctgt tgcaagacct gatacattaa
    10201 atctcccttc ttcatttgtc aaagtaccgg taaccagctc tcccgaacct gcttttacca
    10261 ccgataccgt tgaacttatc agaggctgcc tgtcttccct ggaaagaacc cttcccgaga
    10321 tacttgtctg tgccactagt acttgggtaa ttgctgacag aataacaagt gtaattatga
    10381 tccgccgtga tttcatttca acttgaatta attggtcatt gtatgaatat cataatagac
    10441 catcaccccc aattctttat tccatcctta aggtatattt agtttgattt tttatcaata
    10501 aaaacaataa aaggatatgt caattcatgc tccggtttcc acagtccgga tgttgctttt
    10561 tccggctgtt taattgtaag gttacagact caggtaattt ttaagcagtt ttattcttta
    10621 tatttatagt attgaaaatc acatctggta tgagaaaatt caggcagata attattatcg
    10681 gatcagttat attgctgatc gtttttttgg caatgtttga ctacagtaac ccctggacga
    10741 gatccaacca tgcagttaat gtcggtatag ttgcagtttg tttaaacatt attgcaatga
    10801 tactttccaa caggcacgat gaaaaagcgg ggaaaaaata acaagcgtct gttccagaaa
    10861 gaaaatggca gacgtctgaa tttttcgaat agctgcagtc agaaaatgtt aaaacggtta
    10921 ttggagaacg ttgatccgtt actgaacaat ctgattcata atattatgca aatatgaaaa
    10981 gagagatccc gattttatta cttatagcat tatgtttcgg tacttttgct caggctccgg
    11041 attctttgag agaaaagcca cagttgttac cctactggac cttatgggtt cccggtgcaa
    11101 catattttta tcagggaagg attattcagg gttcggtgtt ttctgcgctg gaactcggtg
    11161 gtatttatac gggtataaaa tatgataaga atttaagggg caatagttca tcaccatatt
    11221 acaactatcc cttactgatg ggattacagg cttttcagac tgagaagctg acaatgttca
    11281 gtaatcagct tgaagtgttc aaatataatt atcctgattt cagatatgat tgcctgtcag
    11341 agaaggattt atatctggct ccgttcaaaa ttaaaaatat aatgactccc ataacagggg
    11401 ggatggtgct gctggccggc gtctttctgg gtattgaaaa attcaatgag acaaagaggt
    11461 tcactgatat tgaaaggatg tattttctga accgttatat cagcagggat cagggattgg
    11521 ctgttttcgg gacggtttca cttgcagcag cctggagtgc cggtatcggt gaggaatatg
    11581 ttttccggaa ctggatgatg cccctgcttg attacaaata cggtcagaaa aagggtttga
    11641 ttatctcaag cgccatcttt gggggagttc attttaccaa tgttttattt gctgaaaagc
    11701 ccgattacaa agctgcatta ttgcaggtag gggaagcagc aattgcagga tatttcctgg
    11761 gccgtgacgt tcagaagaga ggttataata tcggtccggc tgttgcggct catatgtggt
    11821 atgactttac cctgatgctg ggatcatttc tgattaaccc gcagaataat tttctgggag
    11881 tgaatttaaa gttcaggatt taaaaagaat tattgctaaa aatatcatca attcagtaac
    11941 gctgtgcaga gaaagatgaa gacccggctg cttttactcc ccgtaatact gcctgctgcg
    12001 tatgttctga agctgccagg gactttgaac atccagtttt tccggaaagt atttgccacg
    12061 tccgtcaagt ggtgtccatt ccgtaaattc acttaccatc cggccaaggt atggtattgc
    12121 cgtttcaaga cagtcttcaa aatccagatc ttcaggttcc acgatccctc tgcgggggtt
    12181 ttttattgcc cacaggatgg cagcgattgc ccctgccgta acctgtatgg ttgtcgcatt
    12241 ggcgtaatcc acatggcggc gtgcttcttc tatgccgagc tgtgaaccgt accagtatgc
    12301 atttctttca tggcctgcca gcagtactcc gagttcatcc gttccctcca cgatatcagg
    12361 gccgagtctt ctggctgaag cctgcagaac ccatccgctg ccttcaagtt ccagtgcgga
    12421 aagcatggca tcgtcacaag ggtggtatac aaacatcaca gtgggccggt acaggaatga
    12481 atcctcatta cggaccgaca ggtagtcagc aattgacata acctcgtcat gtgttaccag
    12541 cattccctga aatccgccaa ttgagggtac ccagctgcgg gcaaaggtac ttgctcccgg
    12601 acgttccaga tacagtgttc cggagccggg actatgttca catgcaccgc cggggatgcg
    12661 tttctc
//
LOCUS       DAOSPX010000062        12414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5101_length_12414_cov_4.312161, whole genome shotgun sequence.
ACCESSION   DAOSPX010000062 DAOSPX010000000
VERSION     DAOSPX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12414
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5101_length_12414_cov_4.312161"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLZ75_04760"
     CDS             <1..417
                     /locus_tag="PLZ75_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOS71743.1"
                     /translation="KNTLVVLVADHSRRNSPEIQEYSEEIFEIPMLWLGGVLSETGMT
                     VDRTGSQVDIPLTILNQLGLSDHYPFSKDLLSSGSSSFAFYTFNEGFGFITDSSRYIY
                     EHKLGKPVLEAGKSPEIAGKYGKAFLQTLFDDFLER"
     gene            477..1844
                     /locus_tag="PLZ75_04765"
     CDS             477..1844
                     /locus_tag="PLZ75_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS71744.1"
                     /translation="MCSSQIRIYSEKTSPRLDYAAGLIFGDIMGLDWEIVTDESRISR
                     QGIPVINYSRKSIPDSFRIKPVSLLFEKGIIPREISITEWKGLPVFFDADRNPDGGPD
                     SGSAPGSGSDCISEIPFDLLAASFYLVSRYEEYLDYRPDRHGRFPASASAAYRHGFLT
                     LPVVDLWTRELAKELGQKYPGLKFSFSSYHALFTMDSDQPFAYLGKSLFRTAGGLARN
                     MLTDRRKAAERLRVLAGKARDPFDVYDYILRSIAESGSCARFFFPAGSLSEYDRNPNW
                     RNRRYRKLIRKLGNSHVSGLHPSYLASDDLSRFRSERERLGRILNRPVEISRFHFIRM
                     RIPGSYRMISGEGINEDYSMGYPDHPGFRAGIARPFFFYDLGEERKTDLKLVPFQFMD
                     SSFYDYMNLDPEEAEELIIKLVDETRNAGGLFVSLWHNTTLADTYEGRRWRALFEKML
                     VMQKP"
     gene            1841..2746
                     /locus_tag="PLZ75_04770"
     CDS             1841..2746
                     /locus_tag="PLZ75_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71745.1"
                     /translation="MINFYRNCEIDRRRWDACIEKSQGARPYGFSWYLDIMSPGWNAL
                     IDDDYATVFPLPASEKYGIKYVATPVFLQKLGTYSAESPPGKGINEFMGLMYDTYRFI
                     DLCTAQRTDDDRFIVTRKASYELLLEGSYEKLRAGFSVHCRRNIARAQKEKIEIESDV
                     KPSEAVKLFRENAGRRIRGIRPGNYQRLSDLMEYCTGNNRGRILGVRNPAGKLIYGIF
                     ITETGRRKTMLFVVNSGESRLKRIGYYIVNELVRESAGTGTILDFAGSSIPSIASFME
                     SFGSKNIPYYRNYCNRLPWPLRLFR"
     gene            complement(2779..3495)
                     /locus_tag="PLZ75_04775"
     CDS             complement(2779..3495)
                     /locus_tag="PLZ75_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS71746.1"
                     /translation="MQSSLPLDLIIELKDCQIWQQDNLVLSDVNLNVAKGEHIYLVGR
                     VGSGKTSLIKTINAQLPLKHGKATVAGFDLAGLKNRDIPLLRRKLGIVFQDFQLLTDR
                     PVSDNLDFVLRATGWKDRNDINNRIGEVLEKVGLGHKGYKLPHQLSGGEQQRVVIGRA
                     LLNNPDIILADEPTGNLDPETSEGIIRLLIEISKTGRAVIIATHNHTILRKFPARTVR
                     CQDGRLIPVDDDEEIELEKL"
     gene            3697..6702
                     /locus_tag="PLZ75_04780"
     CDS             3697..6702
                     /locus_tag="PLZ75_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS71747.1"
                     /translation="MLISAILSLFSFRAGAQVASSGREYSSEFRRAMEYYNKEKYPAA
                     IRLFDLYLSDPDNDDILSLTDARYYRALSSVKLFNPDAEYQVLTFIKMHPESPRINEA
                     RLALGNYFYQNKNYRKAVTHLEAVNRQELSPEELPEYFFRLGYSHYMRGQKPRALLMF
                     SEIKDTDNEYSVPAVYYYSQIAYEEEMYETALEGFNRLRHNETFGSIVPYYIVQILYL
                     KKDYDQILEIAPGLADKAGKDRANELYRFIGDAYFNKQNYAEALPYLEKFAEGTKTAG
                     REDKYQLGYCYYKAGDYDKAIKVLEEIGVRSDILSQNIWYVLGDCYLRLNNKDRAKFG
                     FAEAAKLDFDRKIQEDALFNFAKITYEISYSPFGEAIKSFQEYVNLFPGSERIEEAYN
                     YLVLAYLQDKNYSAALNSLDKIRNKDARLEEAYQRAAFFRGLEMFNNLAFESAVDMFS
                     KSLQYEKYNRQLRTRAVYWRGEAYYRLGNYDEARKDYVTFLGIPGSLGLNEGSMLRYN
                     LGYTFFNMKDYPNAIVHLKAFESEGGKTKTDLMADARNRLGDCYFIATNYRAAIDYYD
                     KVIDYGQTDADYAMFQKAFSLGLISNERGKADVLSSLITRYPSSSYVPNAIFERGRAY
                     TQLGDYSRGEADFNTIISSHGSSPFVPRALVQLGLLYYNTGQNTRAVEQYKKVIENYR
                     ATPEARSAMTGLRNAYVEMNDVESYFTYARTVQGYGDINMAEKDSLLYASGENLYMSG
                     NYQRAAGVLESYLREFPAGGFVQNARFYLAECRRSEGRDDEALNLYSAVVAAPNNQFT
                     EQSLISAAGLSFDNEDYMASLDYYGKLEAVASSPENRIVSLRGQLRSAYELGDAQKTI
                     AAVDKIGKSAGIPEELQREAIFMRAKANYSLNNFDDALSDFRRTAGEVVSSEGAESKY
                     RIAEILYMKNRNDESERIIQEFIDQKSPHQYWMAKMFLLLSDISVKKGDMLQARVTLE
                     SIRDYYTIRDDGILDDVRTKLAALDQNNL"
     gene            6699..8426
                     /locus_tag="PLZ75_04785"
     CDS             6699..8426
                     /locus_tag="PLZ75_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71748.1"
                     /translation="MKSKFGNMMKRNLLNIFLLILIAADLFSQEQDLKREVTLYNPFI
                     PSLSDFRKKSFLPVIRDTNVVRPEFTYTVNTNPYSPEYTISPIKAAAIVPDPLEKLYK
                     SHVNLGLGNYFAPLAEISVTNERSREGTYGLYAKHYSTHGKVKLQNDKRVFAGYMDNE
                     VSLFGKKFFHDNYLEGSIDYFQKTRHAYGYDTSIVAYEPEKKDIRIPYSKIGANLSFA
                     SMTLDSSDFSYDFDVAYNYFFSDRNMSQNSLGIAGYMAKTYLDLYMGAGLEMDFYRPS
                     AEISRLGRYVVALSPFISKSSAQWSFKLGIQLLLDRDTASTVRFRFYPDAGMAFSIVP
                     SYIDFFARLSGKLEKNTPERIIEENPFLLRNGSLFALPNTSHRLVLSGGLKGNTGMGG
                     NYVLSASYSMIQDMLFYANLVDDGPLPEPMPGNYFLPLPDDVELFNLHAGMNGIIGDK
                     ISYSGSANYYSYRPGTHEYPWGKQPWDAQVGVKYNLRNKIIAGIELTSLGKRRFVVDR
                     SNMSIPQEDYVFTSPVLVSSNLSAEYRYTKILSLWARINNIDFSRNYDWAYYPTQRYM
                     FMIGLTYSL"
     gene            8803..10773
                     /gene="gyrB"
                     /locus_tag="PLZ75_04790"
     CDS             8803..10773
                     /gene="gyrB"
                     /locus_tag="PLZ75_04790"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOS71749.1"
                     /translation="MDDEMNILQEDRKYSAESIQVLEGLEAIRKRPAMYIGDIGVKGL
                     HHLVYEVIDNSIDEALAGYCSRIDIIIHEDGSVTVSDDGRGIPTGIMEKEQKSALEVV
                     MTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSSELTATVCREGKLFRQVYRKGKPLS
                     DVEVLGESDKSGTTITFKPDGTIFQTLDFNYEILAARLRELAFLNKGILLTLKDLREI
                     VENGNGGQPLYTEFFSENGLLEFVQYLDANRERIIEKPIHITSDKTDFPVEIALQYNN
                     SFSENVHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEDSGAAAKLKFDINGDDFREG
                     LTAVISVKVAEPQFEGQTKTKLGNSEVMGPVDQAVSTLLTNYLEENPRDAKIIVQKVI
                     LAATARHAARKARELVQRKNEFSGSGLPGKLADCADRDPANCEIYLVEGDSAGGTAKQ
                     GRDRKFQAILPLRGKILNVEKAMQHRIYESEEIKNIFTALGVNIGTEEDSKALNTSGL
                     RYHKIIIMTDADVDGSHITTLIMTFFFRYMQDLIKGGNLFIATPPLYLVKKGKAERYC
                     WSEEERNEAVRELGQGKDSSVTVQRYKGLGEMNAEQLWNTTMNPETRTLRQVTIDSAA
                     EADHIFSMLMGDEVPPRREFIETHAKYARIDA"
     gene            complement(10784..11896)
                     /locus_tag="PLZ75_04795"
     CDS             complement(10784..11896)
                     /locus_tag="PLZ75_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS71750.1"
                     /translation="MNSIVAIRKCNEYNVTEITDLIADIYESCNGPDPRNKKILLKPN
                     ILSDSDPLKCVSTHPAVVEAMIIYLQSKGATVLVGDSPSIHMRGFRPVNSGITAVCEK
                     TGAEWVDFLLSPSAVKAGGSRIRIASAVRETDLIISLPKFKNHELMLFTGAIKNTLGL
                     VPGFIKARQHAIHQDRDRFAEFLVDLNESVLPHFFLMDAVRGMEGPGPGQGIPVSIGV
                     LIGSANPLALDIIATTLADYDPLEIPTNTVALARGKWLRDPGEIIYDGPEPESLVKKD
                     FKRIIESGSKNTLYSFIFNRFKRIRRIQRRPVFIHRNCTGCMECIRICPVNAIRMHPE
                     KKNHVVLNDRQCIRCFCCSEVCRYHAVEIRRKLFGV"
     gene            complement(11893..>12414)
                     /locus_tag="PLZ75_04800"
     CDS             complement(11893..>12414)
                     /locus_tag="PLZ75_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861015.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOS71751.1"
                     /translation="TADWGYGWSPKRITLSTIGLKNRIREFLDKSRCHLAISLHSPFD
                     DERERLMPVQRSSRIRDVIDIVREYDFTGQRRISFEYILFSGLNDTPAHVRELVRLIS
                     GLKCRINIIRFHEIPGTELKSPDQAEAERFRDALNKKGIPATVRASRGEDIGAACGLL
                     STLEQNKQKKLTE"
BASE COUNT         3434 a         2722 c         3014 g         3244 t
ORIGIN      
        1 aaaaataccc ttgttgttct cgtagccgat cattccagga gaaattctcc ggagatccag
       61 gaatactccg aagaaatatt tgaaattcca atgctctggc tgggaggtgt attgtcagaa
      121 accgggatga ctgttgacag aaccggcagc caggtcgata tccccctcac aatactgaac
      181 cagctgggac tcagtgatca ttaccccttt tcgaaggacc tgctttcttc aggatcttct
      241 tcatttgcct tttatacctt caatgaaggt ttcggtttca ttaccgacag ttccaggtat
      301 atctatgagc ataaactagg caaaccggtc ctggaagcag gaaaaagccc tgaaattgcc
      361 gggaaatatg gaaaggcatt tctccagaca ttgtttgatg attttctgga acgctgagtc
      421 tgacgatatt cactttcacg ctgcaggcag aacgccggat ttagtaaatt tgtatcatgt
      481 gttcctctca gatcaggatt tactcggaaa aaaccagtcc gcggctggac tatgccgccg
      541 gattgatttt tggagatatt atgggacttg actgggagat agttacggat gaaagcagaa
      601 tcagcaggca gggaattcca gtaataaatt attcccggaa aagcataccg gattcattca
      661 ggataaaacc cgtttccctc ctttttgaaa aaggcataat tccccgggaa atttcaatta
      721 ctgaatggaa aggactgcct gtgttctttg atgccgacag gaacccggat ggcggtcctg
      781 actccggttc cgctccgggt tcaggttctg attgcatttc tgaaatcccc tttgatctgc
      841 tggcagcatc cttctatctc gtaagccgct atgaggaata tctggattac cggcccgaca
      901 ggcacggaag attccctgcc tctgcttcgg ctgcttaccg gcacggtttc ctgactcttc
      961 ctgttgtgga tctctggaca agggaattgg caaaggaact ggggcaaaag tatcccgggc
     1021 ttaaattcag tttcagcagc taccatgctc tgtttacaat ggactcggat cagccctttg
     1081 cctatcttgg caagagcctg ttccgcacgg cagggggatt ggcaaggaat atgctgactg
     1141 atcgccggaa agcggcggaa agactcaggg tgctggcagg aaaggcaagg gatccctttg
     1201 atgtttatga ttatattctc cgttccattg ccgaaagcgg atcctgtgca agatttttct
     1261 ttccggcagg gagtttgtcg gaatatgaca ggaacccgaa ctggaggaac cggagataca
     1321 ggaaactgat aaggaaactg ggcaacagcc atgtttcggg tttgcatcct tcataccttg
     1381 catcggatga tctgtccaga ttcagatcag aaagggaaag gctgggcagg attctgaaca
     1441 gaccggtaga gataagcagg tttcatttca tcaggatgag aatacccggt tcatacagaa
     1501 tgatttccgg agaaggaata aatgaggact attcgatggg ttaccctgat caccccggat
     1561 tcagggcagg cattgcacgg cccttctttt tctatgatct gggagaggaa agaaagacag
     1621 acctgaagct agtgcctttt cagtttatgg attcatcatt ctatgactat atgaaccttg
     1681 acccggaaga agcggaggag ctgatcatta aactggtgga tgaaacaagg aatgccggag
     1741 ggcttttcgt cagcctgtgg cacaatacca cacttgctga cacttatgaa ggacggagat
     1801 ggagagcctt gtttgaaaaa atgctggtaa tgcagaaacc atgataaatt tttacaggaa
     1861 ttgcgaaatc gaccgcaggc gctgggatgc ctgtattgag aaatcacagg gagccagacc
     1921 ctacggtttc tcatggtatc tcgacatcat gtctcccggc tggaatgccc tgatagatga
     1981 tgattacgct acggtctttc ccctgccggc atcagaaaaa tacggaataa aatatgtggc
     2041 aactcctgtt tttctgcaga agctcggaac atattcagca gaaagtcccc cgggaaaagg
     2101 aataaatgaa tttatgggct taatgtatga tacttatcgt tttatagatc tctgcactgc
     2161 ccagagaacc gatgatgaca gatttattgt tacccggaag gccagttatg aactgctcct
     2221 tgaaggctct tatgaaaaac tgcgcgcagg attttccgtt cattgcagaa gaaatatagc
     2281 cagggcgcaa aaagaaaaaa ttgaaattga aagcgatgta aaaccatccg aggccgtaaa
     2341 acttttccgg gaaaatgcgg gcaggaggat caggggaata aggcccggaa attaccagcg
     2401 tctcagtgat ctgatggagt actgtaccgg gaataacagg ggaaggatac tgggagtcag
     2461 aaatccggcg gggaaactta tttacgggat attcattact gagacaggga gaaggaaaac
     2521 aatgcttttt gttgtgaatt ccggcgaaag ccgcctgaag cggattggtt attatattgt
     2581 caatgaactt gtgagagaat ctgcagggac cggcaccatt ctcgattttg caggttcatc
     2641 aattccttcc attgcttctt tcatggaatc gtttggcagt aagaatattc cctactaccg
     2701 taattactgc aacaggctgc catggcccct cagactgttc cggtgagaaa cagtgaggcc
     2761 ttgttccgtc cctttattct agagtttttc cagttcgatc tcctcgtcat cgtcaacagg
     2821 tatcagtctg ccatcctggc atctgaccgt acgggcggga aatttcctga gaattgtgtg
     2881 gttatgggtt gcaatgataa cagccctgcc ggttttgctt atttcaataa gcagtctgat
     2941 gataccttcg gatgtttcag gatcaagatt gcctgtcggt tcatccgcca gtattatatc
     3001 ggggttgttc agcagggccc ttcctattac aacacgctgc tgttcccctc ctgaaagctg
     3061 gtggggcagt ttgtatccct tatggccaag gcctaccttt tccagtacct caccgatccg
     3121 gttgttgata tcatttctgt ccttccatcc cgtggcccgg aggacaaaat cgagattgtc
     3181 acttaccggg cggtcggtca gcagctgaaa atcctgaaat actattccga gttttcgcct
     3241 gagcagcgga atgtcccggt ttttaagacc ggccaggtca aagcccgcca ccgtagcctt
     3301 cccatgtttc aggggcagct gtgcatttat cgtctttatc aggcttgtct tgccgctccc
     3361 caccctgcca accagataaa tgtgttcacc ctttgcaaca ttcaggttta catctgacaa
     3421 taccagattg tcctgctgcc atatctggca atccttaagt tctatgataa ggtcaagagg
     3481 aagagaactc tgcatatctg tgtgtttgga ttgtgcaaat taatgaaaga aatggtaaat
     3541 taagccccgc atgttgaaaa aaacaataat aaagaccctc cgaaggcgtt tatataattg
     3601 taattttgca gctgtttgcc ggaacagttg ttggatagcc gttatggcct gaagaggaaa
     3661 aggttaatga tacaaaatat gaataaaaca ggatttttgc taatctccgc tatactttcc
     3721 ctgttctcat tccgggcagg tgcccaggtg gcttcttcgg ggcgcgagta ttcatcagaa
     3781 ttcaggagag ccatggaata ctacaataag gaaaagtatc ctgccgccat caggcttttt
     3841 gatctgtatc tgagcgatcc tgacaacgat gatattcttt cccttactga tgcccggtat
     3901 tacagggctc tttcctcggt gaagcttttc aacccggatg cggaatacca ggtgcttacc
     3961 ttcataaaaa tgcatcctga aagtccccgg ataaatgaag ccaggcttgc cctcgggaat
     4021 tatttctacc agaataagaa ctaccgcaag gccgttactc accttgaagc agtgaaccgg
     4081 caggagcttt cccctgaaga gctgccggaa tattttttcc gcctgggata ttcccattac
     4141 atgcgcggcc agaaaccgcg ggccctgctc atgttctccg aaataaagga tacggataac
     4201 gaatacagcg taccggccgt ctattattat tcccagatag cctatgagga ggagatgtat
     4261 gagacagccc tggaaggctt taaccgtctc aggcataatg aaaccttcgg aagtatcgtt
     4321 ccttactaca tagtacagat cctctatctc aaaaaagatt atgaccagat actcgaaatt
     4381 gcaccaggac tggcagacaa ggcgggaaag gatcgtgcca acgagttgta ccgctttatc
     4441 ggggatgcct atttcaacaa gcagaattat gcggaagcac tgccctatct ggaaaaattc
     4501 gcagaaggga ccaaaacagc cgggagggag gataagtacc agctgggata ctgctattac
     4561 aaggccggag attatgacaa ggcaataaaa gtgctggagg agataggggt aaggtccgat
     4621 atactcagtc agaatatctg gtatgttctc ggagactgct acttaaggct gaacaacaag
     4681 gacagggcca aattcggatt tgcggaagct gcaaaacttg attttgacag gaaaatacag
     4741 gaggatgccc tctttaactt tgcaaagatt acctatgaga tttcatattc cccctttggc
     4801 gaagccataa agtctttcca ggaatatgta aacctgtttc cgggatctga aaggattgag
     4861 gaagcctata attatcttgt tctggcatat ctgcaggaca aaaattacag tgcggcactg
     4921 aattccctcg acaagatccg taacaaggat gccaggctgg aggaagccta tcagcgtgcg
     4981 gcatttttca ggggactcga gatgttcaat aatcttgctt ttgaaagcgc tgttgatatg
     5041 ttcagcaaat cactgcaata cgaaaaatat aacagacagc tcagaaccag ggcagtttac
     5101 tggaggggag aggcgtatta ccgtctggga aattatgatg aagccaggaa ggactatgtt
     5161 actttcctgg gaatacccgg ctcccttggc ctgaatgagg gttccatgct cagatacaat
     5221 cttggctata ctttcttcaa catgaaggat tatccgaacg cgattgttca cctcaaagcc
     5281 ttcgagtcag aagggggaaa gacaaaaaca gatctgatgg ctgatgcaag aaacagactg
     5341 ggtgactgtt attttatagc gaccaattac cgtgcggcca tagattatta tgacaaggtt
     5401 attgactatg gccagacgga cgccgattat gccatgttcc agaaagcgtt ttcacttggc
     5461 ctgataagca atgaacgcgg aaaggctgat gtgctgagtt cccttatcac ccgttacccc
     5521 tcttcatcct acgtcccgaa tgctattttt gaacgcgggc gggcatatac ccagctgggg
     5581 gactattcaa ggggtgaagc agacttcaac accataatca gttcccacgg gtcaagtcca
     5641 tttgtgcccc gcgcccttgt acagctcggg ctcctttact acaacacggg tcagaacacc
     5701 agggccgttg agcagtataa gaaggttatc gagaactaca gggctacacc tgaggcaaga
     5761 agtgccatga caggccttag aaatgcctat gttgaaatga atgatgttga gtcatatttt
     5821 acctatgcaa gaactgttca gggttacggc gatatcaaca tggccgaaaa ggattccctg
     5881 ctctatgcat ccggagaaaa tctctatatg tcaggaaatt accagagggc tgcaggtgtt
     5941 cttgaaagct atctgaggga atttcctgcc ggaggatttg tccagaatgc ccgtttttac
     6001 cttgccgaat gccgcaggtc ggaagggagg gatgatgagg cgctgaatct ttattctgca
     6061 gttgttgcgg ctcccaataa tcagttcacg gaacagtcgc tgatttctgc cgccggcctc
     6121 tcgtttgaca atgaggatta catggcttca ctggactatt acgggaaact tgaagctgtt
     6181 gccagcagtc cggaaaacag gattgtttcc ctgaggggac agctgaggtc ggcatatgag
     6241 ctgggtgatg cacagaaaac aatagcggca gtagacaaga taggtaaaag tgccggtatt
     6301 ccggaagagt tacagagaga agcaattttt atgagggcaa aggcaaacta cagtctgaac
     6361 aattttgatg atgccctgtc agatttccgc aggacagccg gggaagtggt aagctctgaa
     6421 ggggctgaat ccaaatacag gattgcggag atcctatata tgaaaaaccg gaacgatgaa
     6481 tcggaaagga taattcaaga gttcatcgat cagaaatccc cccatcagta ctggatggca
     6541 aaaatgttcc ttttactttc ggacatctcg gtgaaaaagg gtgacatgct tcaggcaagg
     6601 gtaaccctgg aaagcatcag ggattattac acaataaggg atgacgggat tctggacgat
     6661 gtcaggacaa aacttgcagc ccttgaccag aataatctat gaaaagcaaa tttggcaaca
     6721 tgatgaaaag aaatcttctg aatatatttc tactgatact gatcgcggct gatctgtttt
     6781 cccaggaaca ggacctgaaa agggaagtga ccctctataa tccgtttatt ccttcgctgt
     6841 cggacttcag gaagaagagc ttcctgcctg tgatcaggga tactaatgtg gtcaggcccg
     6901 agttcaccta tacagtaaat acaaaccctt acagtccgga gtacacgatt agtcctataa
     6961 aggcggcagc catcgttccc gatccgctgg aaaaactgta taaaagccat gttaatctcg
     7021 ggctgggaaa ctattttgca cccctggccg agataagcgt gacaaatgaa cgctcaaggg
     7081 aaggaacata cggactttat gcgaagcatt actcaacaca cgggaaggtg aaactgcaga
     7141 atgacaaacg ggtgtttgca ggatatatgg ataatgaagt atccctcttc ggcaaaaagt
     7201 tttttcacga taattatctg gaaggttcaa ttgattattt tcagaaaacc aggcatgcct
     7261 atggctatga tacaagcatt gtggcttatg aacctgagaa aaaggatatc aggattccat
     7321 acagcaaaat cggggcaaat ctgtcatttg cttccatgac cctggattca tcagacttct
     7381 cctatgattt tgacgtggcc tataactatt ttttcagcga caggaatatg tcgcaaaaca
     7441 gcctggggat agcaggttat atggctaaga cctacctgga cctgtatatg ggggcaggac
     7501 tggaaatgga tttttaccgg ccgtcagcgg aaatatcccg gctgggaaga tatgttgttg
     7561 ccctcagccc ctttatttcc aaaagttctg cacagtggag cttcaaactc gggatacagc
     7621 tgctgcttga cagggacacg gcatccacgg tgagattccg tttttacccc gatgcaggaa
     7681 tggcattcag catagttcca tcatatattg atttctttgc acggctttca ggaaagcttg
     7741 aaaagaatac gcctgaaagg attattgaag aaaatccttt ccttcttcgt aacggaagcc
     7801 tttttgcact tcccaatacc agccacaggc ttgtgctgtc gggcgggctg aagggcaata
     7861 cgggcatggg gggtaattat gtgctttcag cctcatattc aatgatacag gatatgttgt
     7921 tctatgccaa cctggttgac gacggacccc tgccggagcc gatgcccggc aattatttcc
     7981 tgcctctgcc ggatgatgtt gaattgttta acctgcatgc aggtatgaat ggtattatcg
     8041 gcgataaaat aagctacagc ggaagtgcca attattacag ctacaggccc ggaacacatg
     8101 aatacccatg gggcaagcaa ccctgggacg cacaggttgg agttaaatac aatctcagga
     8161 ataagattat tgcgggaatt gagctgacat ccctcggaaa gaggaggttt gttgttgaca
     8221 gaagcaatat gtcaattcct caggaagatt atgtatttac ctccccggtg cttgtcagtt
     8281 caaatctgag cgcggaatac aggtacacaa agattctttc cctatgggcc agaataaaca
     8341 atattgactt cagcaggaat tatgactggg catattatcc cacccagaga tacatgttca
     8401 tgataggact gacctatagt ctgtaaggca gatatacagt acaataacct gattaccgta
     8461 tttaatatga ccgggaagga cagcagcgga ctttcatctt ttccggttct ttccgattgt
     8521 tgattttttc aggaaattgt gcataaaaac caggtgcttt tcctgtgtga aaagagaata
     8581 tttttgtata tttgcagcca tgattaatga acatgaattc agttatttat cacgtctgaa
     8641 gagagagtat tccttgtgct ggcgattaat ggaatattca gctgttttct cccggaaaaa
     8701 tgcataatta gtcagagcag agcaaagaac ggatgatctc cctcaggaaa ttcatgatgc
     8761 atataatccc taatactgta cctttttaaa agttggcttg aaatggatga tgaaatgaac
     8821 atattgcagg aagacagaaa atattcagcg gagagcatcc aggtccttga aggacttgag
     8881 gcaataagaa agagacctgc aatgtatata ggggacattg gtgtaaaagg tcttcatcac
     8941 cttgtttatg aggttatcga taattcaatt gatgaggcac ttgccggcta ttgctcccgg
     9001 atagatatta taatacatga agatggttcc gtaacggttt cagatgacgg aaggggaatc
     9061 cccacgggga tcatggaaaa ggaacagaaa tcagcacttg aagttgttat gacggtgctg
     9121 catgcaggcg gaaaatttga caagggatcc tataaagtat ccggcggcct tcatggagtc
     9181 ggtgtttcct gcgtaaatgc actttcttca gaactgaccg ccactgtatg ccgggaagga
     9241 aagctgttca ggcaggttta ccggaagggc aaacctctca gtgatgtaga agttctcgga
     9301 gaaagtgaca agtccggaac cacaataacc ttcaagcccg acggaacgat ttttcagaca
     9361 cttgatttca actatgagat ccttgcagcc aggctgagag agcttgcttt cctgaacaag
     9421 ggtattcttc ttacacttaa ggatctgagg gagattgttg aaaacggaaa cggaggccag
     9481 cctctttaca ccgagttctt ttcggaaaac gggctcctgg aatttgttca gtatcttgat
     9541 gccaacaggg aaaggattat tgaaaaacct attcatatca cttccgacaa aacggatttc
     9601 cccgttgaga tagcattgca gtacaacaat tccttctctg agaatgtgca ttcctatgtc
     9661 aataatatca acacaattga aggaggaacc catctggccg gtttccgcag gggactcacc
     9721 agaacactta agaaatatgc agaggactcg ggagctgcag ccaagctgaa gtttgacata
     9781 aacggtgacg attttcgtga aggactcacc gctgttattt ccgtaaaggt tgccgagccg
     9841 cagtttgagg gacagacaaa aacaaaactg ggcaattcgg aagtaatggg tcctgttgac
     9901 caggctgtca gtactttgct gacaaactac ctagaagaga atcccaggga tgcaaaaatc
     9961 attgtgcaga aggtgattct tgccgcaacg gcacgtcatg ctgcccggaa agcacgggaa
    10021 ctggtacaga ggaaaaatga attttcagga tcaggccttc cgggaaagct ggctgactgt
    10081 gccgaccgcg atcctgccaa ttgcgagata taccttgttg aaggggattc agccggggga
    10141 accgcaaaac agggccgcga caggaagttc caggccatcc tgccgctgcg gggcaagata
    10201 ctgaatgttg aaaaagccat gcagcacaga atttatgaaa gtgaggagat aaaaaatatt
    10261 ttcactgcac tcggtgttaa cataggaacc gaagaagaca gcaaggctct caatacttcc
    10321 gggctgagat atcacaagat cataatcatg actgatgccg atgttgacgg ctcacatata
    10381 accaccctga ttatgacctt ctttttcaga tatatgcagg atctgataaa aggcgggaat
    10441 cttttcattg ccactcctcc cctctacctg gtaaaaaaag ggaaggcaga aaggtactgc
    10501 tggtcggaag aggaaagaaa cgaagccgtc agggaactcg gacaggggaa ggactcatca
    10561 gtgacagttc agcgctacaa gggccttggg gaaatgaatg cggaacagct gtggaatacg
    10621 acaatgaatc ccgagacaag gacactgagg caggtgacga ttgacagcgc tgcggaagcc
    10681 gatcatattt tctcaatgct tatgggggat gaagtgcctc cgagaaggga attcattgaa
    10741 acacatgcaa aatatgccag gatagatgcc taggcattgt tattcagaca ccaaataatt
    10801 ttcgtctgat ttcaactgca tggtaccggc atacctcact gcagcagaaa caacgtatgc
    10861 actgcctgtc gttaaggacg acatggtttt tcttctcagg atgcattctg attgcattta
    10921 ccggacatat tctgatgcat tccatacagc cggtacagtt tctgtgtata aataccggtc
    10981 ttctctgaat tcttcgtata cgtttgaacc tgttgaatat aaaactatac agggtgtttt
    11041 ttgaccctga ttcgattatc cttttgaagt ctttctttac cagtgattcc ggttccggtc
    11101 cgtcataaat tatttcaccc gggtccctga gccattttcc ccgggccagg gccacagtat
    11161 ttgtcggaat ttcaagagga tcgtaatctg ccagagttgt tgcgattata tccagcgcaa
    11221 gaggattcgc tgatcctata agaacaccaa tgcttaccgg aattccctgt cccggaccgg
    11281 gaccttccat cccccttact gcatccataa gaaaaaaatg aggcaatact gattcattga
    11341 gatcaacaag aaattctgca aacctgtccc tgtcctggtg aattgcatgc tggcgggctt
    11401 ttataaatcc cgggaccagt cccagtgtat tttttattgc gccggtaaag agcattaatt
    11461 catgattctt gaatttggga agtgatatta taagatcggt ttccctcact gctgaagcaa
    11521 tccgtatccg gctgccaccc gctttcacgg cagaaggcga aagcaggaag tcaacccatt
    11581 ccgctccggt cttttcacaa acggcagtta tgcctgaatt aaccggcctg aaccccctca
    11641 tatgtatgga tggagaatcg cccaccagga ctgtagctcc ctttgactga agatatatta
    11701 tcattgcttc gacaacagcc ggatgggttg atacacattt cagcggatcg gaatcagaaa
    11761 gaatattggg cttcagcaga atttttttat tccgcggatc gggcccgttg cagctttcgt
    11821 aaatatctgc tataagatca gttatttccg tgacattgta ttcattacat tttctgattg
    11881 ctactattga gttcattcag ttagtttttt ctgtttattt tgttccaggg ttgagagcaa
    11941 tccgcatgca gcaccaatgt cttcacctcg ggatgccctt acggtagcag gaatcccctt
    12001 cttgttgagg gcatccctga aacgttctgc ttctgcctga tccggactct tcagttctgt
    12061 tccgggtatt tcatgaaagc ggataatgtt tatccggcat ttaaggccgc tgatgagacg
    12121 gaccagttcc ctgacatgtg ccggggtgtc attcaaaccg ctgaaaagta tgtattcaaa
    12181 ggatattctt cgttgtccgg taaaatcata ttcccttact atatcaatca catccctgat
    12241 ccggctggat ctctgtacgg gcatgagcct ttcccgctca tcgtcgaagg gtgaatgaag
    12301 gcttattgca aggtggcagc ggcttttgtc gaggaattcc cttatcctgt ttttcaatcc
    12361 tatggtactc agtgtgatcc ttttgggact ccatccgtat ccccagtcag cagt
//
LOCUS       DAOSPX010000063        12312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5154_length_12312_cov_4.955780, whole genome shotgun sequence.
ACCESSION   DAOSPX010000063 DAOSPX010000000
VERSION     DAOSPX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12312
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5154_length_12312_cov_4.955780"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLZ75_04805"
     CDS             <1..392
                     /locus_tag="PLZ75_04805"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS71752.1"
                     /translation="DFPVSVEAQFLGGNGVDKRPTLNVCTPGTHIVMNDSLITTHCIN
                     STSKTYHGDVWVSCELVVFADSIIHHIVEGDTVLTYSKPQVGGSNKPADYPVPDGTLI
                     KEGYICLQAESHPVEFRKVELLNLEDK"
     gene            455..538
                     /locus_tag="PLZ75_04810"
     tRNA            455..538
                     /locus_tag="PLZ75_04810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:489..491,aa:Leu,seq:caa)
     gene            complement(554..3202)
                     /locus_tag="PLZ75_04815"
     CDS             complement(554..3202)
                     /locus_tag="PLZ75_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOS71753.1"
                     /translation="MNRQKIFWLSILILLLIPGAFLRAGERDFRISIRNVIQTAPNQL
                     VFDLYILDTDPAQNFHYAMSQIGLMLNSRIYEGGDIYVAIDNRNSTIKPPFTSKPRVS
                     RYSDRTKTYIELDPEVVAYLGSGMIIDKVEPGNLMTRFIITSTVDFAINSTPDIEFTG
                     EIVVFPQFPTKVFAYINRVRTPLPVIPGQNAIVYENPVLNQVRPPVAFEVTGGGSYCE
                     GEEGLPVGLAGSQRGVIYHLYRNGILQDIRVEGTGREISFGIQQDGVYTVTGINRGGE
                     TEMTGNATITANPLPEVPVVSVDCSLGAGKAVVSIISQSARSYEYRLDEGDYQKEPVF
                     RHVLNGSHAITVRNESGCTVTTAPFEVDCPCADPPFIVLSSYSGSTCGTAAVTVSDNS
                     FGGSATSVSLHTSGEGRIFLVSAGSGSFEFVYIPAENDIGKIISIIAETNNPAGPPCE
                     AATAVYALTVGEIPSAPVPGEVIPPSCTVPSGSIALSGLPSAGNWTVTRYPGAHTATG
                     SGTRIVLRGIPPGEYLFTVTNSAGCISPLSDGVNMPDAPDIPSAPVPGEITHPTPDVP
                     TGSVVITGLPSRGLWHLTRNPGNVVTSGTGTTTLVGSLPPGSYTFTVTNEEGCVSRPS
                     AAVVINPQPHAPVLVINNPPTICSTETADLTRPEITAGSDPGLILTYWRDDRAREPLN
                     TPARAPKGTYYIKGTTAAGYFVIKPVIVLADEMPVADSGPDITLAYVFETRLDARVEF
                     GNGMWTVLTGDGRIDDIYDPRSTVRDLSMGQNILLWKVKNGVCPEAVDSLIITVKDFL
                     VPSLLTPNMDGRNDYFMIVGLEKMGRSELVVFNRRGARVYHDRDYQNDWNGVDYNGRL
                     LPDDTYFYVLKTENGQKISGFIVLRK"
     gene            complement(3728..6265)
                     /locus_tag="PLZ75_04820"
     CDS             complement(3728..6265)
                     /locus_tag="PLZ75_04820"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOS71754.1"
                     /translation="MIEKFCSLVAISLFSFNCFSQDTKSFEAVFTDTQLTIDGFLDEA
                     EWQLAPVVSDFIQFEPEEGKPSDRRTEVRVLFGKNDLYIGARMYDDPENIEQNLGRRD
                     EYNRADWFMVSIDSYYNQKTAYTFAVNAAGVQFDGQQDDNKKLVSFNMNPMLPMGLDP
                     SWDAIWFSEVKPGDDGWTAEMRIPYSMLRYSRSQMQNWGIHFTRRIPKNGEVSEWPYI
                     PRNVRMNLVSGYGTITGIRDLDPRRSIQVRPYVLTGLDVFEDKNNPGHSASRWKYGAG
                     ADLKVGLGPNVMLDATIKPDFGQVEADPAVLNLTAFETFFTEKRPFFLEGADIFKFGI
                     GSSRLFYSRRLGASEPIIAAAKLSGHSKKGLSFGLLGTTTGEDFNPTNNYGVFRANQT
                     IGQYSSAGGIVTYYYSPAEKGRGWQSITGGVDWDMRFNRNKYGLEGVFAFSDRNSLEP
                     GRKNEDGFMSALILRKRQGVIDGHLTLLMFSDKYNPNDVGWISYEQNWYQLWSNLSYK
                     INQGKPFGNFQRAMVNMHYSRRLSFIEWYDMGASANLSTNFVTKNFRSFTVGGNFTDL
                     LGGYDLWETRGLGIWAKPAGIGFSGDFSTDDRKNWKIMPSVSAKGDNLRGREYSAGLQ
                     SVFNTGTRLSFQANIRGNWEVNKTAWASNESFLSENSAWKIGNKSLSPDNLEYSDFAE
                     FDDKGQLDEILDGVDQFKPDYYYLPVFGQRDTRSLDLVLRGSLTFTNKFSLQLYTQFF
                     VAKGIYDNFSLLTDPDHMADFSAYPKRRDFNYKNLQSNFVARWEYRPGSTIYFVWSHS
                     RNRRDDMNPLAPWGESAYEQSLGGQLSDLFRIFPGNSFMIKLDYAFF"
     gene            complement(6683..6847)
                     /locus_tag="PLZ75_04825"
     CDS             complement(6683..6847)
                     /locus_tag="PLZ75_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71755.1"
                     /translation="MSVSLKSGDRDIVESFTAGRSLPVSFPPDWAGSDRGINNNSAGR
                     IYSVFMPGGF"
     gene            complement(7357..11343)
                     /locus_tag="PLZ75_04830"
     CDS             complement(7357..11343)
                     /locus_tag="PLZ75_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71756.1"
                     /translation="MAKLELRSIRIRKAPGFRDGLHAPGLDKLAGDINIITGPNGTGK
                     TTIARLIHQLIWPERTVSADVYGSYMINEEPWTVHIDPTARIVQKDGTDSTPSGIPPK
                     ELQKVYMLALHELVGQDDKDLAGRILRESIGGYDLDRASADLKYSGETRNKRSREFTD
                     FNDASRLVAGLSRAHSELKEDEENLSQLEERKLEAERAGELRDLYDKVIQLRQAEQEA
                     AALKISMDAFPAVLNNAAGDEQERIEELERESERENSVVEAATKIIAEEEEKIRELAL
                     PAEGIDDQLLFDLDSRIREIYETGKSIGELENEIIKLETEEIQARESISNIRRTEEWK
                     GLKLEGAQELDVFFHKSLALMQKEISIRTELERLEWEEAAIGENAGTADTAPATAITT
                     DPELTADADKLVKGIDSLGKWLKEARPFRGVQQWLFIVLSLAGILTAAAIFFSGIYGF
                     AGATLIPGILLILGILIYGLLRNSSRNIAGNLKVREDDYARTGLKQPVEWETGTVTAL
                     LDELIGELNKVTGAAEVRRTRQNRIKDLRTGLDRLNVQIREMNNTREMLLGKLRAMPD
                     FPESAEDAISPLYLFLTGLNRWQSAYTSLLSLKESLAGLQKRRNEEIEKCNAVFRKLG
                     INIVVPDDSAKSGAAVTDSGGSGGSDRPDEPVGPGGSDGPGGPDEPGGPDELGRSDGP
                     GGPDEPGGPGRPGELLADSGLFARSITDPDSSDRIRTDSSRTDGDLTDNSRPDGGLTD
                     NSTTDGDLTKTERNDSGYEELRINLSDYSRKARALFQTLSAAENKRREAVREIAGQRR
                     QIENSKARIIDTERKIRALYDKLETQPGNFAEVIRIMEDYPLFRDVKKDYDAASVKLS
                     IKENELKGHSLYPEHEAVLNEISLFEAQTRAEELEKTAAGWRELNEAIISTKTLIEEK
                     KRGAELEAALAAKKNAEDELEKLYTSNLSSMTGFLIAGHLRKETANLSRPEVLKRADR
                     IFSLITKGRYNLRVIDEEGPVFRAIDNVMDLGLTLDQLSTGTRVQLLLATRLAFVETA
                     ENDVRLPVLADELLANSDDERADAIIEALCGISSEGRQVFYFTAQPDEVERWLSYLRR
                     RNDISHKIIQLGGSGEGEGSYDVHKPEIESISFVQKIPEPGNMEMNEYRKVLGIRPYD
                     LIRDDTAGLSLSFLTGDVKLLYDCLSGGVATWGQLHAFHEAGGKIGGTDEKLIAEMQK
                     KTDLLEYFQELYRQGRPRPVGIDVIIESGTVSDRFLDEVKGKLDKAEGNPLKLIAALD
                     TIRNFRADKKEQLRDYLTDRGYIDESPALEEDEIRLRLQARTAELGIIPAEAEKFING
                     LIEFSGSD"
     gene            complement(11345..>12312)
                     /locus_tag="PLZ75_04835"
     CDS             complement(11345..>12312)
                     /locus_tag="PLZ75_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71757.1"
                     /translation="GAGGTWELERYVKGSETLQFAGWSFPVQHVRNSPLKNFDITGID
                     PDLPCIGLLHADYGAVDSPYNPVSLPDFVRPGIDAWILGHIHKPQELNLSGPYIFYPG
                     SPLALSSKETGAHGPVVITVNNNTAGRAERAFPSPVRFENISIDISGSGTEEALRDTL
                     VNGLLDRTLSLGDELQDVSCISYGVMLTGTHANPALVESWASGMRDDFSHETADGTKI
                     VVHDTDFNIQPEVSNLRELARQNSPAGMLARTIVAIEDGEETEFLKNLLAEWKTNHRE
                     MNRKEIYLSLQKSALTDDDAEKKGKQFILAESKRLLGNLLQQKSE"
BASE COUNT         2896 a         3266 c         2679 g         3471 t
ORIGIN      
        1 aggacttccc tgtatctgtt gaggcccagt tcctcggagg aaacggtgtc gacaagcgtc
       61 ctaccctgaa cgtgtgcact ccaggtactc acatcgtgat gaatgattcg ctcatcacca
      121 cccattgtat aaactcaaca tcgaaaacct atcacggcga cgtgtgggtc tcgtgtgaac
      181 ttgtggtctt tgccgacagc atcatccacc atattgttga gggagatacc gtacttacct
      241 actcaaagcc gcaggtggga ggaagcaata aaccggcaga ttatcccgtg ccggacggca
      301 ccctgataaa ggaaggatac atttgccttc aggcagaaag ccatcctgtg gaattcagaa
      361 aggttgagct cctgaacctg gaagataaat aagggttctt ttggcttccg gcttttgccg
      421 tttatatttt tctttataat tttgctccgc ttttgcccgg atggcggaat aggtagacgc
      481 gctggtctca aacaccagtg gcagaaatgc tgtgccggtt cgatcccggc tccgggcacc
      541 tgaatatatc gatctacttc ctcagcacta tgaatccgct gattttctgc ccgttctccg
      601 ttttcagtac gtagaaataa gtatcatcgg gaagtagtct tccattataa tccacaccat
      661 tccagtcatt ctggtaatcc ctgtcatgat acaccctggc tcccctccgg ttaaacacca
      721 ccagttccga tctgcccatt ttttccagac ccacaatcat gaaataatcg ttccggccgt
      781 ccatattggg ggtaagcagt gagggcacca gaaaatcctt tacggttatt ataagggaat
      841 ccacggcttc gggacaaaca ccgtttttta ccttccagag gaggatattc tgccccattg
      901 acaaatccct cacagttgac cggggatcat agatatcatc aatccttccg tctcccgtca
      961 ggacagtcca catgccgttg ccgaattcaa ctcttgcatc cagtcttgtc tcaaaaacat
     1021 aagccagtgt aatgtcgggt ccggaatccg caacaggcat ctcatccgcc aggacaatta
     1081 ccggtttgat cacaaaatat cccgcagcag tagtgccttt tatgtaatat gttcccttag
     1141 gggctctggc aggtgtgttc agaggttccc gggccctgtc gtcacgccag tatgtcagta
     1201 tcagtccggg atccgaacct gcagtgattt caggccgggt aaggtcagca gtttccgtgg
     1261 agcagattgt aggcgggttg ttgattacca gtaccggggc atgaggctgc ggattgataa
     1321 ctactgcagc cgacggacga gagacgcatc cttcctcatt tgtcacggta aatgtatagc
     1381 tgcccggggg aagggagccc accagggttg tggttcccgt gccgcttgta acaacattcc
     1441 ccggattccg ggtgagatgc cagaggcccc gtgacggcag acctgtgatt accacgcttc
     1501 ccgtgggcac atcgggcgtg ggatgagtaa tctctcccgg caccggtgct gagggaatat
     1561 ctggtgcatc gggcatatta accccgtccg acagcgggga gatacaaccc gcggaattgg
     1621 tgaccgtgaa aagatattcc cccgggggaa ttcctctaag aacaatccgc gtaccgcttc
     1681 cggttgcggt atgtgctccc ggataccttg tgactgtcca gtttccggct gaaggcagac
     1741 ctgacagcgc aatacttccc gacggaacag tgcatgacgg cggaatgact tccccgggaa
     1801 caggagctga aggtatttca ccaaccgtca gggcataaac agctgtggca gcttcacatg
     1861 gaggaccggc aggattattt gtctctgcaa tgatactgat tatttttcct atatcatttt
     1921 ctgccgggat atatacaaac tcgaaactgc cggaacctgc tgaaacaagg aatattctcc
     1981 cttcaccgct tgtatgaagg ctcacgcttg tggcacttcc gccgaagctg ttatcactta
     2041 ccgtaacggc ggcagtgccg catgtacttc cggaatagct gctgagaact atgaagggag
     2101 gatctgcaca gggacaatca acctcgaagg gagctgtggt gacagtacat ccggattcat
     2161 tcctcaccgt aatggcatga ctgccgttta atacgtgtct gaaaacgggc tctttctgat
     2221 agtccccctc atcaagccgg tattcataag accttgctga ctggctgatt atgcttacca
     2281 ccgcttttcc cgcccccagg ctgcaatcaa cggaaacaac gggcacttca ggaagaggat
     2341 ttgcagttat tgtggcattt ccggtcattt ctgtctcccc gcccctgttt ataccggtaa
     2401 ccgtgtatac tccgtcctgc tgaatgccga aagaaatttc tcttccggtt ccctcaaccc
     2461 ggatatcctg taatattcca ttgcggtaga gatgataaat cacaccccgc tgtgatcctg
     2521 ccaggccgac aggaagtcct tcctcacctt cacagtagga cccgccgcct gtaacttcaa
     2581 aagccaccgg gggccttacc tggttaagta caggattttc atatacaatg gcattctggc
     2641 cggggatcac aggaagaggg gtccggaccc ggttaatata tgcaaagact ttagtgggaa
     2701 actggggaaa gacaacaatt tctcccgtaa attcaatatc cggagtgctg ttgatagcga
     2761 aatccactgt ggaagtgatg ataaaacggg tcatcagatt accgggctca accttgtcaa
     2821 ttatcattcc ggagcctaaa tatgcaacaa cttccgggtc cagttctata tatgttttgg
     2881 tcctgtctga ataccttgaa acccggggtt ttgacgtgaa aggaggtttg attgttgaat
     2941 tccggttatc aattgccaca tagatatcgc ctccttcata aattcgggaa ttcagcatga
     3001 gtccgatctg actcatggca taatggaaat tctgtgcggg atctgtgtca aggatgtaca
     3061 gatcaaaaac aagctgattc ggagctgtct ggataacgtt cctgattgat attctgaaat
     3121 ccctttcccc ggcacgcaaa aaggctccgg gaatcaggag aagaatcagt atgctcagcc
     3181 agaagatctt ttgtctgttc attgtgcaaa taaatcatca atgacctgct atgaagcttt
     3241 tatgcattca gttattcatg acaggtttta aagttatcac agtccggata agaccagact
     3301 gccggtttca gattaaagtc ctgcaaaaat cgttcccgaa ccaaacagac cgggaacaat
     3361 cagttatttc agggaataaa gttaggcatt cctgtaaaaa tcaacttgct gttaatctga
     3421 tttttgtcaa gtaaaaaagc ctgatcgtca aattttcccc ttattcagct atttttgtat
     3481 ttttcctctg aattaaagtt cccatgaaga aactctcact cattcccgtt gttttactgc
     3541 tgacatccgc cggcttatcc ggccaggatt accgggagac gggaagattc aacacccaca
     3601 tacgggcagg ggattgcttt tgaccgtaca ataaaaaaca ggaagataat ttacggaata
     3661 agcaggaagg ataaactgat catctctttt gaaattgatt agccggaaag tcctgttccg
     3721 gggactatca gaagaatgcg taatcgagct ttatcatgaa gctgttcccg ggaaatatcc
     3781 tgaagagatc tgacagctgg cctccgaggg attgctcata agccgattcg ccccatggag
     3841 caagcggatt catatcatcc ctcctgtttc tcgaatggct ccacacgaaa taaatcgttg
     3901 atccgggcct gtattcccat ctggcaacaa agtttgactg aaggttttta taattgaaat
     3961 cccttctttt cggatatgcg ctgaaatccg ccatatgatc cggatctgtc agcaagctga
     4021 aattatcata aattcccttg gcaacgaaaa actgtgtata gagctggagc gagaacttgt
     4081 ttgtaaacgt aagtgatccc cgcagtacaa gatcgagtga ccttgtgtcg cgctgtccga
     4141 agaccggcag gtaatagtaa tccggtttga actggtccac accgtcgagg atttcatcaa
     4201 gctgcccctt atcgtcgaat tctgcaaaat cactgtattc aagattatcc ggcgagagcg
     4261 acttgtttcc tattttccat gctgaatttt ctgacagaaa ggattcattt gacgcccatg
     4321 ccgttttgtt tacttcccag ttacccctga tgttagcctg aaatgacagt ctggttcctg
     4381 tattaaacac actctgcagg cctgcggaat attccctgcc tctgagatta tccccttttg
     4441 ccgataccga gggcatgatt ttccagttct tcctgtcatc cgtgctgaaa tcgcctgaaa
     4501 accctattcc cgccggtttg gcccatatac cgagcccccg tgtttcccac aggtcatatc
     4561 ctcccagcag gtcagtgaaa tttccgccaa ctgtaaatga tctgaaattt tttgttacaa
     4621 aattggtact gagattggcg cttgcaccca tatcatacca ttcgataaag gacaggcgcc
     4681 ttgaatagtg catgttcacc attgccctct ggaaattgcc gaaaggtttg ccctgattga
     4741 tcttatagct caggtttgac cacagctggt accagttctg ttcatagctg atccagccca
     4801 cgtcgttcgg attgtacttg tcagaaaaca tgagcagcgt cagatgtccg tcaataacac
     4861 cctgcctttt ccttaaaatc agggcgctca tgaaaccatc ctcatttttt cttcccggct
     4921 caagggagtt tctgtcggaa aatgcaaata caccttccag tccgtacttg ttccggttga
     4981 acctcatgtc ccagtcgact cccccggtta tgctctgcca gccccttccc ttttctgcag
     5041 ggctgtagta atatgttact attccgcctg ctgaggaata ttgtccgata gtctggtttg
     5101 ccctgaaaac cccgtagtta ttggtaggat taaaatcttc acccgtggtt gttccaagca
     5161 ggccgaacga cagtcccttt tttgaatggc ccgaaagttt ggctgcggca attataggtt
     5221 cacttgcacc cagcctgcgt gaatagaaca gcctgctgct tccgatgccg aatttgaata
     5281 tatcggcacc ctccaggaaa aagggtcttt tttccgtaaa gaatgtctcg aaggcagtca
     5341 ggttaagaac tgcagggtcg gcctcaacct gtccgaaatc aggttttatg gtggcatcga
     5401 gcattacatt gggtcccagt ccgaccttca ggtcggctcc cgcaccatac ttccacctgg
     5461 aggcagaatg gccgggattg tttttgtctt caaaaacatc cagaccggtc aggacatagg
     5521 gcctcacctg gatactgcgc ctggggtcaa gatccctgat tccggttatt gtcccatagc
     5581 ccgaaacgag attcatcctg acatttctgg gaatatacgg ccattccgac acttcaccgt
     5641 ttttcggtat ccgcctggtg aaatgaatgc cccagttctg catctggctt cttgaatagc
     5701 ggagcatgct gtacgggatt ctcatttcgg ccgtccaccc gtcatctccg ggtttcactt
     5761 ccgaaaacca tattgcatcc cacgaaggat ccaggcccat cggaagcatg gggttcatat
     5821 tgaatgatac cagtttcttg ttgtcatcct gctgtccgtc aaactgaaca ccggcggcat
     5881 tcacggcaaa ggtgtatgcg gttttctgat tatagtagga atctattgag accatgaacc
     5941 agtcggccct gttgtattca tccctgcgtc cgagattctg ttctatgttt tccggatcat
     6001 catacattct ggccccgatg tacagatcat tcttcccgaa taataccctc acctctgtcc
     6061 tgcggtctga gggtttaccc tcctccggct caaactggat aaaatcgctg actacaggag
     6121 ccagctgcca ttccgcttca tccaggaagc catcgatggt gagctgggta tctgtgaaga
     6181 cagcctcaaa ggattttgtg tcctgtgaaa agcaattaaa tgaaaagaga gatatagcga
     6241 caagggaaca gaatttttca atcatggaca ataaaagtgt atatatattt aaaacaggat
     6301 attttcagtt tttttaaaat ttttgcaatt atagcatttt tttcaaatgc gaaactgtcc
     6361 gggaatgcat ggttaccgcc tcagtgcact atattaacat aatttaagaa gccagccctg
     6421 aactttgtgt aactactcct aacctgattt ggaatttgca ctgaattaac gggctggctg
     6481 gttggaaatt ctattgaaaa attctacaat tcctttataa atttacacca aataaaatac
     6541 gaaatcaaat ttttattcat aaaaatgagg ggtttttgtt aatttttttc aattcccgga
     6601 tgctgttttt tccttccgga ttttccgttt ataagtaact gatccggaat gatttttgta
     6661 gtaacttaaa agatcttccg tatcaaaaac cgcccggcat gaaaacactg tatattcttc
     6721 cggcactgtt attatttatt cccctgtcgc tgccggccca gtcaggggga aaagaaaccg
     6781 gcagactacg gcctgccgta aatgattcaa caatatccct gtcccctgat ttaagtgaca
     6841 cactcattca aaaacggccc ggaaagaaag acgaaaatcc ttttattttc aggcaccagt
     6901 gtccggattt ccggccgccg gggggatctg aaacatttcc cgatctgcgg ccaccgcagg
     6961 gttcagagaa atttccgcat tcaaggccac cgcaggattc agataaattt ccgcattccc
     7021 ggccacctca gggttcagag atatttcccc cttccctgcc gcaaccgggt tcggagattt
     7081 atcccggtgc gccgaaattc tacggtaaaa gcaggactca gcccctgccg tatgaaaaat
     7141 catttgttat cccgcacaga attcctctca acagtaaata tcatctcata ataaaggatc
     7201 ctctcactaa aaggattatc aattagtatc agaagtcatt ccctgtaaag cccggtcttt
     7261 ccttttcatc catatgcata tgagactgtc gtttcaatca gtctgaaacc ttctcctgtt
     7321 ctttttaggg tggccggtcg tttacgggag acagggttaa tctgatccgg aaaattctat
     7381 cagaccattg ataaatttct ctgcttctgc cggaatgatt ccaagctctg ctgtcctggc
     7441 ctgcagccgg agtcttattt catcctcttc aagagcaggt gattcatcta tataacccct
     7501 gtctgtcaga taatccctta actgttcctt tttgtctgcc ctgaaattcc ggatggtatc
     7561 cagcgccgca atcagtttca ggggattgcc ttctgcctta tccagctttc ccttcacttc
     7621 atccaggaac ctgtctgaaa ccgttcccga ttcaatgatt acatcaatac ctaccggccg
     7681 cggcctgccc tgccggtaaa gctcctggaa gtattccagc agatctgtct ttttttgcat
     7741 ttccgctatc agtttctcat ctgttccgcc gatctttccg cccgcctcat gaaacgcatg
     7801 aagctggccc catgtcgcga cacccccgga tagacagtca taaagcaatt tcacatctcc
     7861 ggtcagaaag gacagggaca ggcccgccgt atcatcccgg ataaggtcat atggtcttat
     7921 cccgagcact ttccggtatt cattcatttc catattcccc ggttcaggaa ttttctgaac
     7981 aaagctgatg ctctcaattt ccggtttgtg aacatcgtag ctgccctctc cttcaccgct
     8041 acctccgagc tgaatgattt tatggctgat atcattgcgc ctgcggagat atgacagcca
     8101 tctttccact tcatcgggct gtgcggtgaa ataaaacacc tgtctgcctt cactgctgat
     8161 accacacaga gcctcaataa tggcatccgc cctttcatcg tcgctgtttg caaggagttc
     8221 atctgccaga accggaagtc tgacatcgtt ctcggctgtt tccacaaaag cgagtcttgt
     8281 cgcaagcaga agctgcaccc ttgtaccggt tgaaagctga tccagtgtca gtccgagatc
     8341 catcacattg tcaatggccc ggaatacggg gccctcctca tctatgaccc tgagattgta
     8401 gcgccctttt gttatcagac tgaatatcct gtcagcccgt ttcagcacct ccggcctgct
     8461 caggtttgcc gtttcctttc tcagatgtcc ggcaatcagg aagcctgtca tggaggaaag
     8521 attcgaagtg taaagcttct caagttcatc ttcagcattc ttcttcgccg ccagggcagc
     8581 ttcaagctcg gcacctctct ttttctcttc aatcagggtt ttagtactga taatggcctc
     8641 gttaagttcc ctccatccgg ctgctgtctt ctccagctct tcagccctgg tctgagcctc
     8701 aaaaaggctt atttcgttta atactgcctc atgttccggg tatagggaat gccccttcaa
     8761 ctcattttcc tttatgctga gtttcacgga agcagcatca taatccttct ttacatccct
     8821 gaaaagcgga tagtcttcca ttatccttat gacttcagca aagtttcccg gttgtgtttc
     8881 cagtttatca tacaatgccc tgatttttct ctcagtatcg atgattcttg ccttgctgtt
     8941 ttcaatctgt cttctctgac cggcgatttc ccttacagcc tcccgccttt tgttttctgc
     9001 tgctgaaaga gtctggaaca gggctctggc tttccgggaa taatcggaaa gatttatccg
     9061 taattcttcg taaccggaat cgttcctttc agtttttgtt aaatcgccgt cagtcgtgct
     9121 gttatcagtc aaacctccat caggccggct gttatctgtc agatctccgt cagtccggct
     9181 gctatctgtc cggatcctgt cagaactgtc aggatctgtt atagatctgg caaacaagcc
     9241 actgtctgcc aacagctcac ccggtctgcc tggtccacct ggttcgtctg gcccgcctgg
     9301 tccgtctgat ctgcctagtt cgtctggtcc gcccggttcg tctggtccgc ccggtccgtc
     9361 tgatccgcct ggtcctactg gttcatctgg tctgtctgat ccacctgacc cgcctgaatc
     9421 tgttacagct gcaccggatt tagcggaatc atccggaacc actatgttta taccaagctt
     9481 tctgaaaaca gcattgcatt tttcaatttc ttcatttctc ctcttctgta aaccggcaag
     9541 agactctttg agggaaagga ggctggtgta agcagactgc catctattta aacccgtaag
     9601 gaaaaggtaa agcggtgata ttgcatcttc cgcgctttcc ggaaagtcag gcattgcccg
     9661 gagttttccc agaagcatct ccctggtatt gttcatttcc ctgatctgaa cattcagcct
     9721 gtcaagtccg gtcctcaagt ccttaattct gttctgtctc gtccttctca cctctgctgc
     9781 gcctgtcacc ttattcagtt cccctatcag ctcatcaagc agagcagtaa ccgtacccgt
     9841 ctcccactca acaggctgtt tcagaccggt tctggcgtag tcatcctccc tgaccttcag
     9901 atttcctgca atattcctgc tgctgtttct gagcaggcca tatatcagga tacccaggat
     9961 caggaggata cccgggatca gggttgcacc ggcaaaaccg tatatgcctg aaaaaaatat
    10021 tgctgcagct gtcaggattc ctgccagtga cagaacaatg aacaaccatt gctgaacacc
    10081 cctgaacggt ctggcttcct tgagccattt cccgagacta tcaataccct ttacgagttt
    10141 atccgcatca gctgtaagct cagggtcagt tgtaattgcg gtggcagggg cagtatcggc
    10201 agttccggca ttttctccta ttgccgcttc ttcccattca agtctttcaa gttctgttct
    10261 tatgcttatc tctttttgca tcagggcaag agacttgtgg aaaaacacat caagctcttg
    10321 tgccccttca agtttcaaac ccttccattc ctccgttctc cgtatgttac tgatactttc
    10381 ccttgcctga atctcctcgg tttccagttt tattatctcg ttttccagct ccccgatcga
    10441 cttcccggtt tcatatattt cccggatcct gctgtcaagg tcaaacagca gctgatcatc
    10501 aataccctct gccggcagtg ccagctcccg gattttttct tcctcctcgg ctatgatttt
    10561 tgtggcagcc tcaacaacgg aattctccct ttcagattcc ctctccagct cttcaatcct
    10621 ttcctgttca tctccggcag cattattcag cacggcaggg aaggcatcca tactgatttt
    10681 aagagctgct gcctcctgtt ccgcctgacg taactgaatc accttgtcat acaggtccct
    10741 gagctcaccg gctctctctg cctcaagttt cctttcctca agctgcgaca gattttcctc
    10801 atcttccttc agttcactgt gagccctgct taagccggca accagccgcg atgcatcatt
    10861 aaaatccgtg aactccctgc ttcttttatt tcttgtttcg ccggaatatt taagatctgc
    10921 ggaagccctg tcaaggtcat acccccctat tgactcccgg agtatcctcc cggccagatc
    10981 cttatcatcc tggccaacca gttcatgaag ggcaagcata tacactttct gaagttcctt
    11041 tggcgggatc cctgaaggag tgctgtctgt tccgtctttc tgcactatcc gggctgtagg
    11101 atcaatatgg actgtccagg gttcctcatt gatcatatag gaaccgtaaa catctgccga
    11161 aacagttctt tcaggccaga tcagctggtg aataagacgt gcaatggttg ttttccccgt
    11221 accgttcgga ccggtgataa tatttatatc tcccgccagc ttatccaggc ccggggcatg
    11281 cagaccgtcc ctgaagccgg gagctttccg tattctgatt gatctgagtt caagtttcgc
    11341 catgttattc tgatttttgc tgcaacaggt ttcccagaag ccttttagac tcggccagga
    11401 tgaactgctt cccctttttt tcagcatcat catccgtcaa tgcggatttc tgcagactga
    11461 gatagatctc cttcctgttc atttccctgt ggtttgtttt ccattcggca agaaggtttt
    11521 tgagaaactc cgtttcctct ccgtcttcaa tggcaacaat ggttcgtgcc agcattcctg
    11581 ccggtgaatt ttgcctggca agctccctga ggttactcac ctcaggctgt atgttgaaat
    11641 ccgtgtcgtg gacaactatc ttcgtaccat cagcagtttc atgggagaaa tcatccctca
    11701 tacctgatgc ccatgattcc accagtgccg gattggcatg agtgccggta agcatgaccc
    11761 catagcttat acagctcaca tcctgaagct cgtcccccaa tgaaagagtc ctgtccagca
    11821 gtccgtttac aagggtatca cgaagtgctt cctcagttcc ggaaccgctg atgtcaatgc
    11881 tgatgttttc gaagcggacg ggagacggga aagccctttc cgcccttcct gccgtgttat
    11941 tgtttactgt gataaccaca ggtccatgtg cccctgtttc ctttgaactc aatgcaagcg
    12001 gcgatccagg ataaaatata tagggaccgg aaagattcag ttcctgcggt ttgtgaatat
    12061 gacctagaat ccaggcatct atacccggac ggacaaaatc cggaaggctc acgggattgt
    12121 agggactgtc tacggctccg taatccgcat gcaaaagtcc tatgcagggc agatccggat
    12181 caataccagt gatatcaaaa tttttcagcg gactgttcct tacatgctgt accggaaagg
    12241 accatcccgc gaactgcagc gtttcacttc ccttaacata cctttccagc tcccacgtcc
    12301 cgcccgcgcc aa
//
LOCUS       DAOSPX010000064        12268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5179_length_12268_cov_3.424220, whole genome shotgun sequence.
ACCESSION   DAOSPX010000064 DAOSPX010000000
VERSION     DAOSPX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12268
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5179_length_12268_cov_3.424220"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLZ75_04840"
     CDS             complement(<1..832)
                     /locus_tag="PLZ75_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOS71758.1"
                     /translation="MLTNIKITFWINRTKKNSKNLVPVYLRVAHNYENFNRATGIRIP
                     AADWDKKAMRIRGATQEALTANATLNSLKVKVLQIINQLNVSGKPYNIHTIQKILDGK
                     EISQLTVLKIYDEQLAEMKKLKGKDYTAATIIKYANTHLRLKQYMKFKYRRSDMFLYE
                     LNYQFISGFEAFLKQKFNNSTTTCYKHYQRFTRVIHKAMHKGYIEKYPFENYKIKLPK
                     KRIQYLTQEEIDRIDQCDFNVERLNIIRDVFIFACYTGLAYAEVEALTPDNITKGMDG
                     E"
     gene            complement(1165..1560)
                     /gene="ssrA"
                     /locus_tag="PLZ75_04845"
     tmRNA           complement(1165..1560)
                     /gene="ssrA"
                     /locus_tag="PLZ75_04845"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2090..3241
                     /locus_tag="PLZ75_04850"
     CDS             2090..3241
                     /locus_tag="PLZ75_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018966072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS71759.1"
                     /translation="MNKKRIYLSSPHMGGTEEKYVGEAFSSNWIAPLGPNVDAFEKSV
                     AEYCGIEHAVALSSGTAAIHLALIMLGIKAGDEVMASTFTFSATVNPITYVGAIPVLI
                     DSEPGTWNMDPGLLEQAILERSSGNSKRKIKAIIVVHLYGMPANMDRITEIAARYEIP
                     LIEDAAEALGSRFSGIQTGTFGKMGILSFNGNKIITTSGGGALICNDKGLADRARFLS
                     TQARDNAPHYQHSQIGYNYRMSNVLAGIGIGQMEVLEERVKARRNNHFFYKKNLEKFE
                     EISFLNEPGESYFSNFWLTTILIDPARTRVTREELQTALENENIESRPLWKPMHLQPV
                     FSSCPVYQTGVSEDFFNRGLCLPSGSNLTDDDRERIMNVITKTLSRGRR"
     gene            3439..5148
                     /locus_tag="PLZ75_04855"
     CDS             3439..5148
                     /locus_tag="PLZ75_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOS71760.1"
                     /translation="MKYTRTICVLIAVALFSVLTDSSIAFGQKLKPGPQDLSFFSKAD
                     ETDQPYAIYIPQNFDESKKYPLVVFLHGAWSNHRLGLRRVFGQGNLPLPGMNTPGNIQ
                     RESDAEASRYFPPLKDAGYIVAAPYARGTAGYQGIPEQDVYEMLDDIKSRFLIDEDRI
                     YLTGLSMGGGGTIWLGLTRPDIWAAIAPCCPAPPEESGEIACNALNLPVHLFVGDEDF
                     LYKTVLEWKTRFETHTSHLDYAEYPGIGHNSWDYAYRDGFIFEWFSQFKRDMFPREVK
                     FATRWFKYSKAYWVKLDNFTPGTLTTVNAGFAGENNIEIKTDNLEALSLNLAGHPMFN
                     PSKRVSVKIDGKSFSVSTADGVSFMKVNGSWTNRKFTPPLISKRPGAEGPISAAVRSN
                     HIYVYGTGGNPSQEELASRRALAASAADWSGMGGRIMVFPRVLSDKELRESDFDNSNI
                     ILFGTRESNLVIEKLADRLPFHLKDEVKNLGLVYIFPLNDRYVLVNSGLPWWTAPRKS
                     REQGGMAFMGSKIDMLKNYKDFILFRDSPDNIVSEGYFDNNWELPVGAGAAMKNSGVI
                     NLK"
     gene            5173..7188
                     /locus_tag="PLZ75_04860"
     CDS             5173..7188
                     /locus_tag="PLZ75_04860"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HOS71761.1"
                     /translation="MIIRKLQLVTILLVFLVICNSLQARVIRVYVMRTEPYMEGRIFG
                     DAGSYVKIRGQIYGEVDPYDPHNSMIQDIQLAPRNKDGNVEYISDYIIIHPADMSKSN
                     GLLFLSLPNRGNAFDPDSLLLCRGYIYAWCAWQGDVLRGNDRLLMRVPYAGMNGEEIS
                     GIHRTEYQVGTATRTLNLGSGLFTGNSHHSYETVNHDNSDFTLTKRVLEQDERIPVPN
                     SDWAFSDCTYRRFPGAPDEKKISLKDEFQPGFIYELIYEAKNPLVLGLGFAAIRDFAS
                     FIRYETKDTADFPNPLLADKGIANPVKAAVMQGVSQCSNFSRTFLFLGFNQDEKDRQV
                     FDGVNAHVGVRRIALNIRFARPGGVGMQHEDHLFPGNDPPFTWSVETDPVSGIRGGIL
                     EKCLETGTCPKIMQTLSSTEYWQSRASLTTTDSYGTRDLDIPDNVRIYLFSGTQHSPA
                     DAADQASGFRTNPNSFVPYHRALLVALEKWVLDDIEPPPGAYPTIASGTLVPPEREKS
                     GWPDIPGIPYNGRVNELPLLDYGPRYDNRNVSGILTQEPPAAKTDQFYRTLVPAVDRD
                     GNEIAGIRSIDIRVPVGTYTGWALRREGYGRGDLSSLNGMFIPFRINREGRKISKDPR
                     PSFYERYGKEERYMKAVRQAAEDLLREGFLLPEDVNAEIARAIRNIP"
     gene            7333..8469
                     /locus_tag="PLZ75_04865"
     CDS             7333..8469
                     /locus_tag="PLZ75_04865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS71762.1"
                     /translation="MKIILLGTAHPYRGGLASYNERLAGQFLSEGNDVRIYTFTLQYP
                     EIFFPGKTQFTDKPAPEGIKIIRTLNSVNPFSWIRTGRRIKKENPDILLIKYWQPFMA
                     PGFGTVARIAGTGGTKVICIFDNVIPHEKKLFDRLLTGYFIRSIDGAIVMSRSVGEDL
                     AQFRTSVPVIYNPHPLFDNYGEKVPRKDALGKLGLPEDCSYLLFFGFIREYKGLDILL
                     KAFGDKRLREMKLKLLVAGEFYENDQPYRKLVTEAGMDDDVIFFDRFIREDEVSLFFS
                     VADLVVQPYRSATQSGVTQIAYHFGKPMIVTDVGGLSEIVPDGKCGYVVKPEPGEVAG
                     AIIDFFNNERQSFFTEGVENERSRFTWDRLTASILEVYRLILKR"
     gene            8748..9782
                     /locus_tag="PLZ75_04870"
     CDS             8748..9782
                     /locus_tag="PLZ75_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOS71763.1"
                     /translation="MIFRSKAPLRLGLAGGGSDVSPYSEMYGGAVLNATIDQYAYCTI
                     EENGSGQVTLIAADLDQTGQYPASLNLPVDGHLDLHKGVYNRIVQKFGITEPLSFRMT
                     TWCDVPPGSGLGTSSTMVVAIVKAFAEWLNLPLGDYDTALLAYQVERLDLGLSGGKQD
                     QYAATFGGFNFIEFTKDERVIVNPLRMKRWIIDELEASMVLFYTGESRSSDRIIREQQ
                     DNTREGKKLPLEATHRIKENSFIMKELLLKGEIVRFARTLGQEWENKKKMALSISNRK
                     IDRIYQAAMDAGAYGGKVSGAGGGGFMFFMTDPVRRFALLSSLERFNGRVVNFRFSEN
                     GCQGWRIQNG"
     gene            9889..10587
                     /locus_tag="PLZ75_04875"
     CDS             9889..10587
                     /locus_tag="PLZ75_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS71764.1"
                     /translation="MEVIILAGGLGTRLREVIRDIPKPMAPVAGRPFLHYILDWLRRY
                     PVRKIIISAGYRAEVIREHFGSRFYEIPLEYVIEEKPLGTGGAVNYALGKTAGNDILV
                     LNGDTWFPVDPCRLMGFHSSEDSRFSLALKRMFDFDRYGTVSLEGNRVLGFNEKKACD
                     EGLINGGIYVINRKFFESQKLPEVFSLEKDFLEKNAGKSVLKGMIFGDPFLDIGIPED
                     YAKAEEVLGKYFQK"
     gene            10691..11143
                     /locus_tag="PLZ75_04880"
     CDS             10691..11143
                     /locus_tag="PLZ75_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOS71765.1"
                     /translation="MNKALFLDRDGVINVDRGYVFRIDDFEFIPGIFELCRKYQSEGY
                     LIIVVTNQAGIARELYSEEDFEGLTDWMTDRFRDRGIEIAKFYHCPHHPYITGPCDCR
                     KPEPGMLLQAVRDFDLDISQCVLVGDNESDLEAGRRAGIKEENLHLFV"
     gene            complement(11217..12170)
                     /locus_tag="PLZ75_04885"
     CDS             complement(11217..12170)
                     /locus_tag="PLZ75_04885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS71766.1"
                     /translation="MDLSVVIPLYNEEESISELTEWIERVCVSANISFEIIIIDDGSS
                     DSSWERISELAAARKFIRGIRFRRNYGKAAALHTGFGQAAGDVVITMDADLQDSPGEI
                     PELIRMIKEDGYDLVSGWKKKRYDPFIKRYTSRIYNGTARLASGIRLHDFNCGLKAYR
                     NDVVKSIEVYGEMHRYIPILAREAGFLNIGEKIVEHHPRKYGDTKYGPGRFVKGYLDL
                     LTVGFITRFGKNPMHLFGSLGTVMFLIGFIMAACLGIRKLVFVSRGLRAPLVTDSAYF
                     YIALTVMIIGSILFLTGFLGELINRTSTERNHYLLKDKVNL"
BASE COUNT         3405 a         2538 c         3052 g         3273 t
ORIGIN      
        1 actctccgtc cattcccttt gtaatgttat caggggttaa cgcttccacc tcagcgtaag
       61 caagcccagt gtaacaggca aagatgaaaa catccctgat gatgttcagg cgttccacat
      121 taaaatcgca ctggtcgatg cggtcaattt cttcttgcgt aaggtattgt atccgtttct
      181 tgggaagttt tatcttataa ttttcaaacg gatatttttc gatatatccc ttatgcattg
      241 ctttgtggat aacacgtgta aaacgttgat aatgcttata gcatgtcgtt gttgaattat
      301 tgaatttctg tttgagaaac gcttcgaagc cgcttataaa ttggtagttc aattcataca
      361 ggaacatatc gcttctacgg tatttgaatt tcatgtactg tttaagacgc agatgggtgt
      421 tggcgtattt gatgatggtg gcagcagtgt agtccttacc cttcagtttt ttcatttctg
      481 caagctgttc atcatagatt ttaagcacgg tcagttgaga gatttccttg ccatcaagga
      541 ttttctggat agtgtgaata ttataaggct tcccgcttac atttaattga ttaataatct
      601 gaagaacttt tactttaagt gaattaagtg tagcattggc ggttaatgcc tcttgggtag
      661 caccccttat tctcattgcc tttttatccc aatcagcagc aggtatcctg ataccagtag
      721 ccctgttgaa gttttcataa ttatgtgcca cacgtaaata cactggcaca aggtttttcg
      781 aattcttctt tgttctgtta atccagaagg tgattttaat gtttgttagc atagcataaa
      841 atgttaaatt ttgataaagt cgattaacgg cattctatgc tggtttgcga acatcaaatt
      901 agccaattcg taacccaaaa cgggtatttg gaaggatagg tacaagaatt ggctaatttt
      961 attcactcat tttttaacat ttaaggtagc ggctgcaaaa aaataatgcc tttgtaagtg
     1021 ctgaaatcgt gagagttgcg caggtgaaat gttgcgtttg cccagtatat gcacgcttgc
     1081 gttgcatgaa gtagcggaaa cgtccagtat acctgcgctt cagtggttat aaaaacaaaa
     1141 ccacactcgt tggaatgtgg ttcttagtgg agctggcggg agtcgaaccc gcgtccaaac
     1201 acgatgacca tatgctttct acatgcttag tcctgcttgt ttttcgtgac ctgccagctg
     1261 cagaacaagc taaccggccc ttagttcctg attttctcgt catccgaccg gaacaatctt
     1321 ctgaccagcc ccgaattccg tgcgcctcct tgccgggttg gatcagggcc tgaccgcccg
     1381 ggagacgtcc cgtcccggaa cctggttccg ggataaagct taagttactt tattcgctta
     1441 agcagcgaga gcgtaattat attcgccagt tattgttttg tgacactgtt caagcgacgc
     1501 gccaccacac gctgcatgct tacataccac ccctcatgct gtcaaaacca agtcaacccc
     1561 tgagctgttg caggccggac taatcccgaa atacccttaa aacttccttc cgggcggatc
     1621 agttcccgga cattgccggt cctcttcaac ttattgcctg aataattgtt tcttttatca
     1681 ggttgaagta tgccgtcact gccggcctgc aggtatgcaa ttctttgcaa agatacgatt
     1741 ttaataactt aaccggaaat cattatcaaa aatcagacca tttgatgata tagtgaaacc
     1801 agtttgattt ttaaccgtaa aatatttccg gtccgtgagg aaccggatgc ccgcatatga
     1861 cttgaaaaac gaatttaaaa acggactgat cagagtcaac agggatttta taagtgagac
     1921 aaaatgaaag aataaacaac cttctgtaag tgaattcttt ttgtgaaggg aagtttccgt
     1981 gttttaaaga ttatgttata gttaatcatt attttgatga agtttcatta ttacttttac
     2041 ctgtaaaatt accaggatat cgtattcttc aatatattag caaagatcaa tgaataaaaa
     2101 gagaatatat ctgtcttccc cgcacatggg gggtactgag gaaaaatatg tcggggaagc
     2161 tttcagcagt aactggattg cacctcttgg accaaatgtg gatgcttttg aaaagtcagt
     2221 cgctgaatat tgtggaattg aacacgcagt tgcactgagc tccggtaccg cagccataca
     2281 tctggctctt ataatgctgg gaatcaaagc cggagatgag gtgatggcat caacatttac
     2341 tttttcggca acggttaatc ccatcacgta tgtgggtgca attccggttc tgattgacag
     2401 tgaacccgga acctggaata tggatcccgg ccttctggaa caggccatac tggagcgcag
     2461 ttccggtaat tcaaagcgga aaataaaggc aataattgtg gttcatctgt acggaatgcc
     2521 cgccaatatg gacaggataa cggaaatagc cgccagatat gaaattccgc ttattgagga
     2581 tgctgccgag gcactgggaa gcagattcag tggtattcag acaggtacct ttgggaaaat
     2641 gggaatttta tcctttaacg gaaacaagat aataaccact tcaggtggcg gggcattgat
     2701 ctgcaatgat aagggactgg cagacagggc caggttcctg tcaactcagg cacgtgacaa
     2761 tgctcctcat tatcagcata gccagatagg gtataattat cgcatgagta atgtactggc
     2821 cggaataggt atcggtcaga tggaggtcct ggaagaaaga gtgaaagcga gaaggaataa
     2881 tcatttcttt tacaaaaaga atcttgaaaa gtttgaagaa attagtttcc tgaatgaacc
     2941 cggggaatca tatttttcga acttctggct gacaaccatt ctgattgatc ctgcccggac
     3001 aagggtgacc agggaagaac tgcagactgc cctggaaaat gagaacattg aatcgcggcc
     3061 gttatggaaa cctatgcatc ttcagcccgt tttttcatcc tgcccggtat accagactgg
     3121 tgtttcggaa gactttttca acagggggct ctgcctgcct tccggatcaa accttacaga
     3181 tgatgaccgt gagagaataa tgaatgtaat tacaaaaacc ctgtcacggg gcagaagatg
     3241 atgaatgttt cattgtaatt gcagagggag aatgtagtaa ctccgaaaaa ttcttattgc
     3301 cccgggcaac tttattttcc acagttttta tatcacggat tatgtttata tccgggaatt
     3361 atgtttctga aatattgaac aataatattc aaattagtgt atttttaaat caggaatcat
     3421 aattaacagt cagtcgtgat gaaatatacc agaaccattt gtgtcttaat tgcagttgcc
     3481 ctgttttctg tattgacaga cagtagtatt gcctttggcc agaagctgaa accgggccct
     3541 caggatcttt ccttcttctc aaaggccgat gaaaccgatc agccttatgc aatctatatt
     3601 ccgcagaatt ttgatgaatc gaaaaaatat ccgctggtgg tttttctcca tggtgcatgg
     3661 tcgaatcatc gtcttggcct gaggcgggtt tttggccagg gaaatcttcc cctgcccggt
     3721 atgaatactc cgggaaatat acagagggaa agtgatgccg aggcttcaag atatttcccc
     3781 ccgctgaagg atgcaggata tattgtcgct gctccctatg caaggggaac agccggttac
     3841 cagggcatcc ccgagcagga tgtgtatgag atgctcgatg atatcaaaag ccgttttctg
     3901 atagatgagg acaggatcta tcttacagga ctttcaatgg gaggcggcgg caccatctgg
     3961 ctgggtctga cacggccgga tatatgggct gcaatagctc cctgctgtcc cgctccgccg
     4021 gaagaatcgg gagaaatagc atgcaatgcc cttaatctcc cggtacatct ctttgttggt
     4081 gatgaagact ttctgtataa aacggtgctg gaatggaaga caagatttga aacccatact
     4141 tcgcaccttg attatgccga atatcccgga ataggtcata acagctggga ttatgcctac
     4201 agggacggat ttatatttga atggttctcc cagttcaaaa gggatatgtt tccccgggaa
     4261 gtgaagtttg caacaagatg gttcaaatac agtaaggcat actgggtgaa actggacaat
     4321 tttactccgg gaaccctcac cactgtcaat gcagggtttg caggagaaaa caatattgag
     4381 ataaagactg acaacctgga ggctttaagc ctgaacctgg caggacatcc catgttcaat
     4441 ccttcgaaaa gagtttcggt gaagatcgac ggcaagtctt tcagtgtcag tacggctgac
     4501 ggagtgtcat ttatgaaggt taacggcagc tggacaaaca ggaaatttac tcccccgctc
     4561 atatcaaagc gtccgggagc cgagggacca ataagcgctg ccgtgagaag caatcatatt
     4621 tatgtttatg gaacgggagg caatccgtca caggaagaac ttgcttccag aagagctctt
     4681 gctgcctctg ctgccgactg gtccggaatg ggcggcagga taatggtatt tcccagggtg
     4741 ctttcggata aggaattgag ggagagtgat tttgataata gtaatatcat tcttttcggt
     4801 accagggaaa gcaatctggt gattgagaaa ctcgccgaca gacttccttt tcatctgaag
     4861 gatgaggtta agaatctcgg cctggtatat attttcccac tgaatgaccg ctatgttctt
     4921 gtaaattccg gacttccctg gtggaccgct ccgcggaaaa gcagggaaca gggaggaatg
     4981 gcatttatgg gctcaaaaat agatatgctg aaaaattata aggatttcat cctcttcagg
     5041 gatagcccgg acaatattgt atcagagggt tactttgaca ataactggga actgcccgtc
     5101 ggtgcagggg cagcaatgaa aaactccggg gtcataaatc tgaaatgatc acggaattat
     5161 atagcaaaaa acatgatcat cagaaagtta cagttagtta ctattttact tgtttttctg
     5221 gtaatttgca actccctgca ggccagggtt atcagggtat atgtcatgag gacggaaccc
     5281 tatatggaag gcaggatttt tggagatgca ggttcctatg tgaagataag aggtcagatc
     5341 tatggtgaag tggatccgta tgatcctcac aacagtatga tccaggatat tcagctggca
     5401 ccacgaaaca aggatggcaa tgtggaatat atttccgatt acataattat ccatcctgcg
     5461 gacatgtcga aaagcaacgg tcttcttttt ctgagtctgc caaacagggg aaatgccttt
     5521 gatcctgatt cgctgctgct gtgcagggga tatatctatg catggtgcgc ctggcagggc
     5581 gatgttctca ggggaaatga ccgccttctt atgagagtgc cctatgccgg gatgaacgga
     5641 gaggagattt ccggtatcca tcgaactgaa taccaggttg gcacagcaac caggacatta
     5701 aatctgggca gcggcctgtt cacgggcaat tctcatcaca gttatgaaac agtcaatcac
     5761 gataattctg atttcacact gacaaagcgg gttctggaac aggatgaacg gataccggta
     5821 cctaacagcg actgggcatt ttcagattgc acataccggc gttttccggg cgctcccgat
     5881 gaaaagaaga tttccctgaa ggacgaattt cagccgggat ttatttatga gctgatttat
     5941 gaagcgaaaa atcccctggt actcggactg ggttttgctg caatacggga tttcgcatct
     6001 ttcatcaggt atgaaacaaa agatacggcg gattttccca atcctctgct tgcagataaa
     6061 ggtattgcaa atccggtaaa agcggcggta atgcagggtg tgtcgcagtg cagcaatttc
     6121 agccgtacat ttctgtttct gggttttaac caggatgaaa aagaccggca ggtatttgac
     6181 ggagtgaacg cacatgtcgg ggttcgccgc attgcattga atatcagatt tgccagaccg
     6241 ggaggtgtgg gaatgcagca tgaagatcat ttattcccgg ggaatgaccc tccttttaca
     6301 tggagtgtcg aaactgaccc ggtttcagga ataagaggcg ggattctgga aaaatgcctt
     6361 gaaacgggta cctgtccgaa aataatgcag acactcagtt caaccgagta ctggcagtcc
     6421 cgggcttccc tgacgaccac cgattcatac ggtacaaggg atctggatat tcctgataat
     6481 gtcaggatat atctgttttc aggtacccag cattctcctg ccgatgctgc tgaccaggca
     6541 agcggattcc ggacaaatcc gaattcattt gtgccctatc accgggctct tcttgttgca
     6601 ctggagaaat gggtgctgga tgatattgaa ccgcctccag gtgcttatcc cactattgct
     6661 tccgggactc ttgttccgcc ggagcgggaa aaaagtggct ggcctgatat ccccggaatt
     6721 ccgtataacg gaagggtgaa tgaactgcct ctgcttgatt acgggccccg gtacgataac
     6781 aggaacgtgt caggaatcct tacgcaggaa ccgccggccg ccaagacgga tcagttttac
     6841 cggacccttg tgccggctgt agacagggac ggtaatgaaa tagcgggaat cagaagcatt
     6901 gacatcaggg tgcctgtcgg aacatataca ggctgggcac taagaaggga aggatatggc
     6961 aggggagacc tgtcatcact gaacggtatg ttcattccgt tcaggattaa cagggaaggc
     7021 aggaagatat caaaagaccc cagaccatct ttctatgaaa gatacggaaa agaggaaagg
     7081 tatatgaagg ctgtcagaca ggcagctgaa gacctgctaa gggaaggctt tctgctgccc
     7141 gaagatgtaa atgctgaaat agcccgtgca atacggaata ttccctgatt gcccgggacg
     7201 gtctgaaacc agcactgcac cctgaaacca gctctgcacc ctgtaaccca tatcccggtg
     7261 ccggaatccg gatggtctcc taagtctgta tccggcattt ttgctaattt tgctgtcagt
     7321 tacattggtc tgatgaaaat tattctgctt gggactgcac atccttaccg tggaggcctg
     7381 gcttcgtaca atgaacgcct tgcggggcag tttttgtcgg agggcaatga tgtacggata
     7441 tatacattta cattgcagta tccggagatt tttttcccgg gaaaaacaca attcactgat
     7501 aagccggcac cggaaggtat taaaataatc cgtacgctga attcggttaa tcccttcagc
     7561 tggataagga caggacgcag gataaaaaag gagaaccccg acatacttct cataaagtac
     7621 tggcagccat ttatggcccc gggtttcggc actgttgcca ggattgcggg aacaggcgga
     7681 acaaaagtca tctgtatttt cgacaatgtg atcccccatg aaaaaaaact gtttgacaga
     7741 ctgcttaccg ggtattttat acgcagcatt gacggtgcca ttgtgatgtc acgaagtgtg
     7801 ggagaagatc ttgcacagtt ccggacatcc gttccggtta tttacaatcc ccatcctctt
     7861 tttgacaatt atggtgagaa ggttccccgg aaagatgcac tcggaaaact cggcctgccc
     7921 gaagactgtt catacctgtt gttttttgga ttcatcaggg aatacaaagg acttgatatt
     7981 cttttgaagg catttggtga caagcgtttg agggaaatga aactcaaact gctggtagcc
     8041 ggtgagtttt atgaaaatga tcagccatac aggaaactgg ttacagaagc cggcatggac
     8101 gatgatgtga tattttttga ccgcttcatc agggaggacg aggtctccct gtttttcagc
     8161 gttgccgatc ttgttgtcca gccttaccga agtgccacgc agagcggtgt gacgcagata
     8221 gcctatcatt tcgggaagcc gatgattgta acggacgtcg ggggactgag cgaaatagtc
     8281 cctgatggca agtgcggata cgtggtaaag cctgaacccg gggaggttgc cggtgcgata
     8341 attgattttt tcaacaatga acggcaatct ttttttaccg aaggagttga aaatgaaaga
     8401 tcaagattca catgggacag gctgactgca tcaatacttg aggtgtacag attgatattg
     8461 aagagataat cgtaaatcct gaacggcgta taagaggaaa aattaaacgg attaatcgta
     8521 taagcgggta aacgggtaaa cgaataatag agtattgaat aattgccagg ctgatgaaac
     8581 gactgacggg gtaaaagaat aacgggacaa tctgctgcct ggctgaaagt taaatgacaa
     8641 atgattcctt gaaatatctg ccagatgatt aactggactg gcgaatcatt taataataag
     8701 gtaattattg accggataat tgagaagaac aaaaaataac agagtatatg atttttcgaa
     8761 gcaaggctcc cctgaggctt ggactggcag ggggaggcag tgatgtttca ccatattcgg
     8821 agatgtatgg cggcgccgta ctcaatgcaa caattgacca gtacgcatac tgtaccatcg
     8881 aggaaaatgg ttccggtcag gtaaccctga ttgctgcaga cctggatcag accggacagt
     8941 atccggcatc actgaacctg ccggttgacg gtcatctcga cctgcacaag ggtgtttaca
     9001 accggattgt acagaaattc ggcatcacgg aaccgctctc attcagaatg acaacatggt
     9061 gtgatgtacc tcccggaagc ggtctgggaa catcttccac catggtggtt gccatcgtaa
     9121 aggcatttgc agaatggctc aacctgccgc tgggggatta tgacacggct ctgcttgcct
     9181 accaggttga aaggcttgac cttggcctga gtggaggtaa acaggaccag tacgcagcca
     9241 ctttcggggg attcaatttt attgaattca caaaggatga aagagttatt gtcaatccgc
     9301 tcagaatgaa aagatggatc attgatgaac tggaagcatc catggtattg ttttatacgg
     9361 gggaatcaag atcatctgac cggataatcc gtgaacaaca ggacaatacc agggaaggaa
     9421 aaaaactccc gctggaggcc acccacagga ttaaggagaa ttcctttatt atgaaagaac
     9481 tgctgcttaa aggtgagatt gtccgctttg ccagaaccct gggacaggaa tgggagaata
     9541 agaaaaaaat ggctttgtcg atttccaacc ggaaaatcga caggatctat caggctgcaa
     9601 tggatgcggg agcttatgga ggaaaggtat ccggggcagg agggggagga ttcatgtttt
     9661 ttatgacaga tcccgtgcgc agatttgcgt tgcttagctc actggaacgc tttaacggca
     9721 gggtggtgaa tttccgtttc agtgagaacg gatgccaggg atggagaata cagaatggat
     9781 gaggaacaca gggtgaatga ggaatacaga gaggatgagg aacacagggt ggttgaggaa
     9841 tacagggagg atgaagtcag agtcagaact taatttattc ataatccgat ggaagtgatc
     9901 atactggcag gaggattggg aacacgactg agggaggtga tacgggatat tcccaaacca
     9961 atggcaccgg ttgccggcag gcccttcctt cattacatcc ttgactggtt acggcgttat
    10021 ccggtaagga agataatcat ttccgcagga tacagggcgg aagtgataag ggagcatttc
    10081 ggaagccgtt tttatgaaat tccacttgaa tatgtgattg aggagaaacc actcggcacg
    10141 ggaggagcgg tgaattatgc actcggaaaa acggccggga atgacatcct tgtattgaac
    10201 ggggacacct ggtttccggt tgatccctgc aggctgatgg gattccattc atctgaagac
    10261 agccggtttt cccttgcact gaagagaatg tttgattttg acaggtatgg tacagtgagc
    10321 ctggaaggca atagagttct cggattcaat gaaaagaaag cctgtgatga gggcctgata
    10381 aacgggggaa tatatgtaat aaacaggaaa ttctttgaat cacagaagct gccggaagtg
    10441 ttttcactgg agaaggattt cctggaaaag aatgccggaa aatctgttct gaaagggatg
    10501 atcttcggtg accccttcct tgatataggt attcctgaag attatgcaaa agcagaagag
    10561 gttcttggaa aatattttca aaagtagaga aatagcacag gtgttttttc agcagaaaca
    10621 aaaagcgtct gcctttcctt caaagcctgt ctgaacttat tgttttaaga taaattaact
    10681 gaaaattgac atgaataagg ctctgtttct ggatcgtgac ggggtgataa atgttgacag
    10741 gggttatgtt ttcagaattg atgatttcga attcattccc gggatttttg aactgtgcag
    10801 gaaataccag tctgaaggtt atctgattat cgttgtcaca aaccaggccg gaatcgcgag
    10861 ggaattatac agtgaagaag attttgaagg gctgacggac tggatgacgg accggttcag
    10921 ggacaggggc attgagatag cgaaattcta tcattgtccc catcatccgt atattaccgg
    10981 accatgcgac tgccgcaagc ctgaacccgg aatgctgctt caggccgtaa gggattttga
    11041 tctggacatc tcacaatgtg ttcttgtcgg ggataatgaa tccgaccttg aagccgggag
    11101 aagagccggg ataaaggaag agaacctgca tctctttgta taaaactccc cttgggggga
    11161 tcagtcgtgt ccggtactaa acaggtgtat gtcaggaaga tagcgaatcc ggagtattac
    11221 aggtttacct tatcttttaa aaggtagtgg tttcgttcgg ttgatgtcct gttgatcagc
    11281 tctccgagga agcccgtcag aaagaggatg gagccgatta tcatgactgt aagtgcaata
    11341 tagaaatatg cactgtccgt gacaagcggg gctcttagtc cgcggctgac gaatacaagt
    11401 tttctgattc caagacaggc tgccataatg aatccgatca ggaacatgac agtgcccagg
    11461 gatccgaaca ggtgcatggg atttttgccg aagcgcgtta tgaatcccac ggtaagcagg
    11521 tcgagatagc cttttacaaa cctgccgggt ccatatttgg tgtctccgta tttcctgggg
    11581 tggtgttcaa caattttttc ccctatattc aggaatccgg cttccctggc cagtatcggt
    11641 atatagcggt gcatttcacc atatacctct atgcttttga caacatcatt ccggtaagct
    11701 ttcagtccgc agttgaaatc atgaaggcgg atcccggagg ccagcctggc ggttccgttg
    11761 tatatccggc tggtgtagcg tttaatgaaa ggatcatatc tctttttctt ccagcccgaa
    11821 actagatcat atccgtcttc ctttatcatt cttatgagtt ccggtatttc accgggactg
    11881 tcctgaaggt ctgcatccat tgtaatgacc acatcaccgg cagcctgacc gaaacctgtg
    11941 tgaagagctg cagccttgcc gtaattcctt ctgaacctga tacccctgat gaatttcctg
    12001 gcggcggcaa gttcactgat cctttcccat gaagaatctg aactgccgtc atcaataatt
    12061 attatttcga aagaaatatt tgcactgacg cacactcttt caatccattc cgtcagttct
    12121 gaaatagatt cctcttcgtt gtaaagcggt atgacaaccg acagatccat ctgaaaaata
    12181 ttcaggaatt aattaacggg attgtcaaca agcggattgc cttcccttct tacaaaagca
    12241 gcaacgataa gcgacataat cagtccct
//
LOCUS       DAOSPX010000065        11975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5305_length_11975_cov_5.261326, whole genome shotgun sequence.
ACCESSION   DAOSPX010000065 DAOSPX010000000
VERSION     DAOSPX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11975
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5305_length_11975_cov_5.261326"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLZ75_04890"
     CDS             complement(<1..504)
                     /locus_tag="PLZ75_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOS71767.1"
                     /translation="MKRNIKSFSRMNKGILILTAVLLFQGIHFSVTAQQRAQVRYRGG
                     GSDRPLNIICSFSDFATITEYIAGNRAQVSYIASGERDPHFVPPKPSYAVMLSRADMW
                     IATGMDLEMWSATLLDKAHNPRIMDGEPGFVAAAQGVNVVEKVEFASRSEGDIHLMGN
                     PHITTGPL"
     gene            complement(523..1875)
                     /locus_tag="PLZ75_04895"
     CDS             complement(523..1875)
                     /locus_tag="PLZ75_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71768.1"
                     /translation="MTKYIINLSILLFVISIFDLGAQRQNVVYVSEQDSLLKLKEELK
                     LLKTEYIDIASRFDSLIQAIDRKEKAAQAENDLQKLLDEANRMAALEKEKEEDLAVKF
                     QSGVRQQQALNPNISLGGDFFGAISTSGHEFISEPGEISYGNNGFFLREIEVGIEAPL
                     DPFTRGKSFISITSDAINIEEAYMEWLNLPLNMNLKIGIFYTEYGPINRYHDHALPQF
                     DRPRALVNLFSNQGLGGTGVSTTFMLPRLLFSDASSLDLSVQKGTPTEDIFSFGNKGF
                     LYSGQFKNYYDISQNSYFEVRLSNVTGRNPVPGNNLSSVGSLGMSYKWVPAGREKYRT
                     LDWKSEFLYSYRREHTGSIRSIGFYSTVQNKLNARFTAGARIGYSQLPYDNKQYEWDY
                     TVNLDFWQSEFVFARIQYQYNRRNLTDFQPDPPGMMPNDHSVILQVCWAMGPHKHEAY
                     "
     gene            complement(1944..2321)
                     /locus_tag="PLZ75_04900"
     CDS             complement(1944..2321)
                     /locus_tag="PLZ75_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71769.1"
                     /translation="MLKLTKIARIFTPLFLVIVIGYITNATINQHSHKLSSGIVVTHS
                     HPFNNGGTGTPFQDHKHGSEELILLSQISISLFRIYLFIIILSTTAAISVIKTPFQQN
                     SSGTSGLFFLNNYHAPPPSTPGF"
     gene            2511..3302
                     /locus_tag="PLZ75_04905"
     CDS             2511..3302
                     /locus_tag="PLZ75_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS71770.1"
                     /translation="MQTQIKISVLTDNHAGSFFPAEHGLSYLIEYDGKKILFDTGQSD
                     MFLRNAEKLNAGLDNTDMTVLSHGHFDHGNGLAFLPGGKLVCHPGCFVRRFRGQDGTY
                     IGLGMTEEEISSKFDLKTSADPLQISGKIIFLGAIPRITDFESRKTTFRFEDGTADFV
                     EDDSALAVILPEGLFIISGCGHSGIVNTCEYARKITGISKLHGIMGGFHLKTTATPTR
                     ETIKYLSETGAKHVFPSHCTELPALAAFYEAFAIRQIKTGDVLVF"
     gene            complement(3311..4156)
                     /locus_tag="PLZ75_04910"
     CDS             complement(3311..4156)
                     /locus_tag="PLZ75_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOS71771.1"
                     /translation="MATNPQSKPMNDLFEKIPMEGIRNIIVVASGKGGVGKSTVAVNL
                     AVTLARNGLSTALFDADIFGPSVPRMFGLEDVRPEVSAVGDKEIFFPVKKFGVNIISI
                     GFFIDQSQSLIWRGPMAGKAVTQLLGDTQWGETDYLIIDFPPGTSDIQLTTVQKLNLS
                     GAVIVTTPQEISLNDARKAASMFNNPDLNVPILGVIENMSWFTPEKHPDEVYYIFGKG
                     GGLRLAKEFNTELLGQIPLVMEVGEAADQGLTVFSQKNKPVVKAFEQIAESIIAKTEK
                     REAGK"
     gene            complement(4126..4998)
                     /locus_tag="PLZ75_04915"
     CDS             complement(4126..4998)
                     /locus_tag="PLZ75_04915"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS71772.1"
                     /translation="MEIAIVSGKGGTGKSSISAAFATAGGRVVIADCDVDAANLYLIF
                     NPVDEEETVYVSGHNAVINYDLCTNCGLCTDYCRFDAISAGDDGIVTISGISCDGCFL
                     CSRVCPEKAIEMIPNDKSRMYSGSFRNGRMVYGKLAPGEENSGKLVNMVREKAKETAR
                     ENNIGTIILDGPPGIGCPAISTITGADKVVIVTEPTISGLSDLKRMAEVTGKFSLQAL
                     AIINKYDLNTEISGQIEEWCSQNGIRVIGKLPFDSRITEAMIQSKSITEYNPQLDISN
                     KLKEIWQQILNQNQ"
     gene            complement(4973..5851)
                     /locus_tag="PLZ75_04920"
     CDS             complement(4973..5851)
                     /locus_tag="PLZ75_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS71773.1"
                     /translation="MIKIAIASGKGGTGKTFVSTNIFYALTRENISSVLADCDAEAPN
                     VLSFFRTVRKETHEVVQHVPVIDVSKCTFCGKCHEYCNYNAIFILPPSGIISVIEDLC
                     HGCGACSVACEYDAITEKPFPLGNVNISLYNNSHPVIEARMNTGIMSPVRVIKEALRH
                     IRHEAEIVILDSPPGTSCPFIQTVSQADYVILVTEPTPFGLSDLRQSVETLRTMDSKF
                     GVIINKAGMGNNDVREYLKKEGIDLLLEIPYSRETAGLYSAGRLLAEADESFGKQLLN
                     VTEYITGKYGNSNSQR"
     gene            complement(5887..6225)
                     /locus_tag="PLZ75_04925"
     CDS             complement(5887..6225)
                     /locus_tag="PLZ75_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOS71774.1"
                     /translation="MKKRIAVPLENGILCTHFGHCQQFAIIDTNENIITEEQLITPPP
                     HEPGLLPGWLAEKGVTDVIAGGMGQRAINLFNQQKINVFVGAPVKTPNELASDLLSNS
                     LKAGANYCDH"
     gene            complement(6296..6712)
                     /locus_tag="PLZ75_04930"
     CDS             complement(6296..6712)
                     /locus_tag="PLZ75_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOS71775.1"
                     /translation="MFNIPYIRDMFIGMIRRPKPRNAGRINKFNIIMKVAITSTGSTS
                     DSKLDPRFGRCSYFVIYDTDTREMEFIRNPNKDATEGAGPASVQTVASRNVKKIISGE
                     FGTKIRSLLDSLGIEMTAIRDPEKSISDIVEMLGQQ"
     gene            6964..7938
                     /locus_tag="PLZ75_04935"
     CDS             6964..7938
                     /locus_tag="PLZ75_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS71776.1"
                     /translation="MNLRLLRRVAVMLIISAVLGFNLNGQTRSDVIKVYNEGAKTVKT
                     DVNASIEAFENVIKLAAEVGETCDDLRDRAIQILPGLYVRLANDALSEKKPAAELIKA
                     SKTAVAAAEKYNNETHRENANKLLVQGYYNLGNEYFNTRDYDNALLVYDSLLAINPDY
                     TAAIFNKALLYRNQDDAENFEGTIDLCIEKASAANDTARAQQASGVALEYFRAAGSRA
                     NQADNLEEALGLLNKSAKYGDDKDLFYYFADVYNKQKNYNSGAEYAQKGLDMETGDAE
                     AKAKFYYQLAVAQAGKGQNAEACASFKNAMYGPFLEASKAQRTNLKCQ"
     gene            8105..8506
                     /gene="nikR"
                     /locus_tag="PLZ75_04940"
     CDS             8105..8506
                     /gene="nikR"
                     /locus_tag="PLZ75_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOS71777.1"
                     /translation="MSVVRFSVSLEEKLLKELDDYVGNNNFANRSQGLRYLIEKNIVE
                     EKWHCNNIVAGAVIIIYDQQKKDIITKSNDVRHNYFDVILSSQHFYLNQDICLEVIAV
                     RGKAKRLTELADKLIAIKGIRHGKLLMSKTD"
     gene            complement(8531..9958)
                     /locus_tag="PLZ75_04945"
     CDS             complement(8531..9958)
                     /locus_tag="PLZ75_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOS71778.1"
                     /translation="MPFLDETILFETMNIYTFFLSLIAAMGGFLFGYDTAVISGTISQ
                     VALQFGLDSIQTGWYVGCALVGSIIGVSFAGILSDYLGRKNTMFISAVLFTVSAAGCA
                     IASGFNGLVVYRIIGGMGIGVASIVSPVYISEISAARHRGSMVALYQLAITIGFLAAY
                     LFNYLLLGYSVRSSFASPLMHKIFNAEVWRAMLGLETLPALLFIILIFFVPESPRWLI
                     LKNRVEKSRSIIYRIHGNRQDTEEEINSILESTGRETKSEWTMLLKPGIFKAVLIGVA
                     IAMLGQFMGVNAVLYYGPTIFEKSGLSTGDSLFYQVMVGLVNMGTTVIALLIIDRVGR
                     KKLVYWGVSGMIISLILIGLYFLYGEKAGLASLFLLAFFLLYIFCCAISICAVIFVLL
                     SEMYPTRVRGLAMSVAGISLWIGTYLIGQLTPWLLENLTPSGTFFLFAAMCFPYMWLM
                     WKKIPETTGKSLEEIEEYWLGPTGA"
     gene            complement(10096..11109)
                     /locus_tag="PLZ75_04950"
     CDS             complement(10096..11109)
                     /locus_tag="PLZ75_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71779.1"
                     /translation="MANVIIGIHGLGNKPPEEILATWWKAAMAEGLKNSGHDSAMIRF
                     SLAYWADILYDKRQDPSEKDPVSPYYMDEKYNKASGDFTGEDYSTRKKVIDFLGRQMN
                     RIFLNEDLTLNYSFITDAIIHRYFNDLEIYYSGHILDIKKNALLNAGELIRERLLSLL
                     KAHSKDEIMLIGHSMGSIIAYDVLTFLAPDIRINTLVTMGSPLGLPVIISRTAAEAGQ
                     RGMDNKQLATPPGVIRKWYNFSDLHDKVSFNYRLSDHYSENAHGVKPFDILIINNYES
                     NGIRNPHKSFGYLRATQFTEILNEFILSEKLSLVQKAGRSISRMTRSLRSLISPAGQK
                     TRQ"
     gene            11245..11703
                     /locus_tag="PLZ75_04955"
     CDS             11245..11703
                     /locus_tag="PLZ75_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71780.1"
                     /translation="MKEEYRLGRLFGQALIYAGYFLIVSGVIISCFSIAGLLLVLAGI
                     FAVFTYEGTIIDFERRRLKNYICLFGWVKAGRWYSVDHFRKFTIYTKGRRSTGRSRRN
                     APSNLTDRNIRLALVSRDGSRKIVVNRYDTFDAARREMNELIKDLKLKEA"
     gene            complement(11779..>11975)
                     /locus_tag="PLZ75_04960"
     CDS             complement(11779..>11975)
                     /locus_tag="PLZ75_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71781.1"
                     /translation="GGPGPGGGWGRAMAPGQGRGLGPAKGWGRGLGPGQGQGLAPGGR
                     FFKDENKNGICDFYEESVKK"
BASE COUNT         3234 a         2692 c         2694 g         3355 t
ORIGIN      
        1 aagcggaccg gtggtaatat ggggattccc cataaggtga atatctcctt cagaacggct
       61 tgcaaattca actttttcaa caacattgac tccctgggca gcagcaacaa atcccggttc
      121 accgtccata atcctggggt tatgtgcctt gtcaagaaga gtagctgacc acatttcaag
      181 atccatacct gtggcaatcc acatgtcagc ccttgaaagc atgaccgcat aactgggctt
      241 gggaggaaca aaatgaggat cccgttcacc gcttgcaata tagcttacct gggccctgtt
      301 gccggcaatg tattcagtaa tggttgcaaa gtcggaaaaa gaacagataa tgttgagcgg
      361 tctgtcggat ccgccgcctc tgtatctgac ctgagccctt tgctgggccg ttacgctgaa
      421 atgaattccc tgaaacaaga gtacggccgt cagtatcagg atccctttat tcattcttga
      481 aaaactcttt atatttcttt tcatattatt aaaataagat cgttaataag cttcatgttt
      541 atgggggccc attgcccagc atacctgcag aataaccgag tggtcattcg gcatcatgcc
      601 aggcggatct ggctgaaaat ccgtcaggtt tcgccggtta tactgatact gaattcttgc
      661 aaaaacaaat tcgctctgcc agaagtcaag attcacggta taatcccatt catactgctt
      721 attgtcatag ggcagctggg agtagcctat tctggcgccg gctgtaaatc ttgcattcag
      781 cttgttctga acggtggaat aaaatccaat gcttctgata ctgccggtat gttccctgcg
      841 gtaactgtat aaaaattcgg atttccagtc aagggtccgg tatttctcac ggccggccgg
      901 aacccattta tatgacatgc caaggcttcc gacgctggac aggttgtttc ccggcacagg
      961 atttctgcct gtcacattgc ttagtctgac ttcaaaatag ctgttctgac ttatatcata
     1021 ataattttta aactgccccg aatacaggaa gcccttattg ccgaaactga aaatatcttc
     1081 cgtgggagta cccttctgaa cactcaggtc cagggagctt gcatcagaaa aaaggagcct
     1141 tggcagcata aaagtcgttg aaacacctgt tccgccgagt ccctggttgc tgaaaaggtt
     1201 taccagggcc ctcggcctgt caaactgggg cagtgcatga tcatgatacc tgttgatggg
     1261 accgtattcg gtatagaata taccgatttt aagattcatg ttcagaggaa ggttcagcca
     1321 ttccatatag gcctcctcaa tgtttatcgc atcactggta atggaaataa atgatttccc
     1381 ccgggtaaaa ggatcgagag gagcttcaat gcccacttct atttcacgca ggaagaatcc
     1441 gttgtttcca taggatattt caccgggttc ggaaatgaat tcatgacctg atgtactgat
     1501 ggccccgaaa aaatccccgc cgaggctgat attcggatta agtgcctgct gctgccttac
     1561 cccgctctgg aattttaccg ccagatcctc ctccttttcc ttttccagcg cggccatacg
     1621 gttggcctca tcaagcagct tctgtaaatc attctcagcc tgagcggctt tttccttccg
     1681 gtcaattgcc tgaataaggg aatcaaacct tgaagcgatg tcgatatatt ccgtcttcag
     1741 aagctttaat tcttctttta gttttaagag tgagtcctgt tcgctcacat aaaccacgtt
     1801 ttgccgctga gcgcccagat caaaaatgga tatcacaaat aaaagaatgg acagatttat
     1861 aatatattta gtcatactta tacaacagtc agttgtttag ttgattaggt gaataataaa
     1921 tgattagatt gtcttgccgt ccttcagaaa cccggagtgc ttggcggagg tgcgtgataa
     1981 ttattcagaa aaaaaaggcc ggatgttccg gaagaattct gttgaaaagg ggttttaata
     2041 actgatatgg cagcagtggt actcagaatg attataaaaa gataaatacg gaagagtgaa
     2101 atggagatct ggctcaggag tattaattcc tcgctaccat gcttgtgatc ctgaaaggga
     2161 gtccccgtac cgccattatt gaaaggatgg gaatgagtga ccacaatccc tgaagataat
     2221 ttatgggagt gctggtttat agttgcattt gttatatagc caataacaat aacaagaaac
     2281 agtggtgtga agatccttgc tattttagtc agctttaaca attttatcct ttaatgaggt
     2341 ggctggtaaa tttaaatttc tttaatctct ttacaaactt attaagattt agttaagata
     2401 aaaatagagg catagtaata aatgttaaat ttacataaat gatattgctt cagaatacag
     2461 tatttcaggg taaatctaca tgaacagtta ccatatattc atacacacag gtgcagacac
     2521 aaattaaaat atcagttctt acagacaacc acgcgggatc tttttttcct gctgagcatg
     2581 gtctttcata tttgattgaa tatgacggaa agaagatact ttttgatacc gggcagagtg
     2641 atatgtttct caggaatgct gagaaactga atgccggcct tgataatacg gatatgacgg
     2701 tattgagtca tggccacttc gatcacggga acggacttgc ttttctgccc ggggggaaac
     2761 ttgtttgtca tccgggatgc tttgtcaggc ggttcagagg ccaggacggt acttatatag
     2821 gactgggaat gacggaggag gaaatatcct caaaatttga tctgaaaact tctgcggatc
     2881 ccttgcagat cagcggaaaa ataatttttc tcggagcaat accgaggatt acggattttg
     2941 agtccaggaa gacaacattc aggtttgaag atggcactgc tgattttgtt gaggacgaca
     3001 gcgcacttgc cgttattctc cctgaaggcc tgtttataat aagcggatgc ggtcattccg
     3061 gtattgtaaa cacctgtgaa tatgccagga agattacggg gatcagcaag ctgcacggaa
     3121 taatgggagg atttcacctg aaaacgaccg caaccccgac cagggaaaca ataaaatatc
     3181 tcagtgaaac gggagcgaaa catgttttcc cttcacattg tactgaactt ccggcactgg
     3241 cagcttttta tgaagctttc gcaatccgcc agatcaaaac cggagatgta ctggttttct
     3301 gacagcaggt ttatttccct gcttcccttt tttcggtttt tgcaattatt gattccgcta
     3361 tctgttcaaa agcctttaca accggcttgt ttttctgact gaatacagtc agtccctgat
     3421 ccgctgcttc acccacttcc ataaccaggg gtatctgtcc gagcagttct gtgttgaatt
     3481 cctttgccag ccttagtccg ccgccttttc cgaaaatata atatacttca tccggatgct
     3541 tttcaggtgt aaaccacgac atgttttcaa taaccccgag aatgggaaca ttgagatcgg
     3601 gattattgaa catggacgct gctttacggg catcgttgag tgatatttcc tgcggagtgg
     3661 ttacaatgac tgctccggaa agattcagtt tctgaacggt agtaagctgg atatcactgg
     3721 ttcccggagg aaaatcaatt atcaggtaat cggtttcacc ccactgggta tcacccagaa
     3781 gctgtgtgac tgcttttccc gccatgggtc cgcgccatat aagtgactgg ctctgatcaa
     3841 taaaaaagcc tattgaaatg atgtttaccc cgaatttctt taccgggaag aaaatctcct
     3901 tatccccgac tgctgatact tccggcctga catcttccag tccgaacatt ctgggaactg
     3961 acggaccgaa tatgtccgcg tcgaacaatg cggtactgag accgtttctg gcaagggtga
     4021 ctgcaaggtt gacggctacg gttgattttc cgactccgcc cttgccggat gccacaacaa
     4081 taatgtttct gatcccttcc atgggaattt tttcaaaaag atcattcatt ggttttgatt
     4141 gaggatttgt tgccatattt ctttcaattt attactgata tcaagttgcg gattatattc
     4201 cgtaatgctt tttgactgta tcattgcttc cgttatgcgg ctgtcaaagg gaagttttcc
     4261 gataacacgg attccgttct gactgcacca ctcttcaatc tgaccgctta tttcagtatt
     4321 gaggtcatac ttgttgatga tggccaatgc ctgcaggctg aatttccctg taacctctgc
     4381 catccttttc aggtcagaca atccggatat ggtcggttcc gttacgatga caaccttgtc
     4441 ggcaccggtt atggttgaga ttgcagggca gcctattccc gggggaccat ccaggatgat
     4501 tgtgccgata ttattttcac gggctgtttc ctttgccttt tcgcgtacca tgtttaccag
     4561 cttccctgaa ttctcttccc cgggggcaag ctttccgtaa accattctgc cgtttcggaa
     4621 agatcctgag tacattctgc ttttgtcatt ggggatcatc tcaatggctt tctcgggaca
     4681 aacgcgtgaa cagagaaagc agccgtcaca ggaaatcccg gagatggtta caattccatc
     4741 atccccggct gatattgcat caaagcggca gtagtctgta cataaaccgc agttagtgca
     4801 caggtcatag tttatgacag cattatgacc tgatacatat acagtttctt cttcatcaac
     4861 aggattgaat atcagataaa ggtttgccgc atcaacgtca caatctgcaa tgacaacccg
     4921 gccgccggcc gttgcaaacg ccgcactaat gctggatttg cccgtaccgc ccttaccgct
     4981 gactattgct atttccatat ttcccggtaa tgtattcagt aacattcagc aattgtttcc
     5041 cgaaggattc atcagcttcc gcaagcagcc tgccggcgga atacagtccg gcagtttccc
     5101 tgctgtaagg tatttccagt aaaaggtcta tgccttcttt tttcaggtat tccctgacat
     5161 cattgttgcc cattcccgct ttattgataa tcacaccaaa cttactgtcc attgttctga
     5221 gtgtttccac gctctgtctg aggtcactca ggccgaatgg agtcggttcg gtaacaagta
     5281 ttacataatc agcctggctg actgtctgga taaaaggaca ggatgttccc ggaggagagt
     5341 ccaggatcac tatttcagcc tcatgcctga tatgcctgag agcttccttg ataaccctaa
     5401 ccggtgacat aatacccgta ttcattcttg cctcaataac agggtgtgaa ttgttgtaca
     5461 gagaaatatt aacatttcca agcggaaaag gtttttcggt aatggcatca tattcacagg
     5521 caacactgca ggcaccgcag ccgtggcaga gatcttcaat cacactgatt attcctgacg
     5581 gcggcaggat gaatatggca ttgtaattac agtattcatg gcattttccg cagaaagtgc
     5641 acttgctgac atctatcacc ggcacgtgct gtacaacctc atgagtctcc ttcctgacag
     5701 tcctgaagaa tgaaagtaca ttcggggctt cggcatcaca gtctgctaga acggaactga
     5761 tattctcccg ggtcagagca taaaagatat tagtggaaac gaaggttttt cccgttccgc
     5821 cttttccgct ggcaatagca atttttatca ttatggtaca ggccaggtca aaatgcctgc
     5881 gtaatttcag tgatcacaat aatttgctcc cgctttcagt gaattgctga gcagatcgct
     5941 cgccagctca ttcggcgttt tgaccggtgc accgacaaat acgtttattt tctgctgatt
     6001 gaataaatta atggcccttt gacccattcc gcccgctatc acatccgtaa ctcccttttc
     6061 tgccagccag cccggaagaa gacccggttc atgaggaggc ggggtaataa gttgttcctc
     6121 tgtaataata ttttcgtttg tgtcaataat ggcaaattgc tggcaatggc cgaaatgtgt
     6181 acataatatc ccgttttcaa gcgggacggc tattcttttt ttcatctttg ttttggtttt
     6241 gtttgacaaa ggcctcccgg tgcattccat catggccgct tcatgtgtat atgttttatt
     6301 gctgaccgag catttctaca atatccgata tgcttttttc aggatcccgg attgctgtca
     6361 tctctattcc gagactgtca agcagggatc ttatcttggt accgaattct cctgatatga
     6421 tttttttaac attccgggaa gcaactgtct gaaccgatgc cggacctgca ccttccgtcg
     6481 catccttgtt gggattccgg atgaattcca tttcccgggt gtcagtatca tagatgacga
     6541 aatacgaaca tctgccgaat ctcggatcaa gtttgctgtc agatgtactg cctgtactgg
     6601 taatggctac tttcatgatt atgttgaatt tgttaatcct tcccgcgtta cggggcttcg
     6661 gccgccttat catgccaatg aacatatccc ggatgtacgg gatattaaac attcatgccc
     6721 gcagatccct gatgaaaggg ggccggcccg gactgcaaag ctaatgaaca tttgttcata
     6781 attggaaaaa agcagtaatt ttaacaaatt ttgaccaatg catgatttaa ttatttaaaa
     6841 aatatcatgt taagtgagca gtatggttat ttttgctttt gtggcaggct gaaaaccgga
     6901 ccttataaat tttatttaat aaatttgctg actttgatta ttaattaata aatctgattt
     6961 gttatgaact tacgtttatt aagaagggtt gcagttatgc tgatcatttc cgccgtactt
     7021 ggtttcaatc tcaacgggca gaccagaagc gatgttatca aggtttacaa tgaaggggca
     7081 aaaaccgtta aaaccgatgt gaatgcttcc attgaagctt ttgaaaatgt cattaaactt
     7141 gcagccgaag taggcgaaac ctgtgatgat cttagggaca gggcaataca gattctgccg
     7201 ggattgtatg tcagactggc taatgatgct ctcagtgaaa agaagcctgc ggcagaactg
     7261 attaaagcat ctaagactgc agttgccgcc gcagaaaaat ataacaatga aacgcacagg
     7321 gaaaacgcaa ataaactgct tgtacaggga tactataacc tcggcaatga atattttaat
     7381 accagggact atgataatgc cctgctggtt tatgattccc tcctggccat aaatcctgac
     7441 tacactgcgg caatattcaa taaggcattg ctgtacagga atcaggatga tgcagaaaat
     7501 tttgagggga ccattgatct ttgcattgaa aaggcctcag cagcaaatga cacggcaaga
     7561 gctcagcagg catccggagt tgcacttgaa tatttccgtg cagccggctc aagggccaat
     7621 caggctgata atctcgaaga agccctcggc ctgcttaata aatccgcaaa atatggagat
     7681 gacaaggatc tgttctatta ttttgccgat gtgtacaaca agcagaaaaa ttacaacagc
     7741 ggtgcggaat atgcacagaa gggcctggat atggaaaccg gggatgctga agccaaggca
     7801 aagttctatt accagcttgc cgtggcacag gcggggaaag gccagaatgc ggaagcatgt
     7861 gcttcattta agaatgccat gtacggacct tttcttgaag catcaaaggc acagagaacc
     7921 aatcttaagt gccagtaata cccggacggg aacctgcgga atttaccgtt gctttacaca
     7981 gtggccggcc ggcggcgaaa aggccgtaag catgataacg gttcctgacc gcccgccgga
     8041 aagcatcagg gaaataattc cggctcccgg cagataatgc ccgccggatc aaatttgtgt
     8101 tacgatgagt gttgttcgat ttagtgtaag ccttgaggaa aagctgctta aggagctgga
     8161 tgattacgtc ggaaataaca atttcgccaa ccgttcacag ggcctgaggt accttattga
     8221 aaaaaatatt gttgaggaaa aatggcattg caacaatatt gttgcgggcg cagtgatcat
     8281 catctatgat cagcagaaga aggatatcat aacaaagtca aatgatgtca ggcataacta
     8341 tttcgatgtt atcctttcat cccagcattt ctacctgaat caggatatat gcctggaggt
     8401 cattgcagtc aggggtaagg ctaaaaggct gactgaactg gccgacaagc tgatagccat
     8461 aaagggcatc aggcacggga agctcctgat gagcaaaacg gactgatatg atttccgttt
     8521 accggggaag tcaggcaccc gtcgggccaa gccagtattc ttcaatttcc tcaagtgatt
     8581 ttccggttgt ttccgggatc tttttccaca tcagccacat ataggggaag cacattgctg
     8641 cgaaaaggaa aaatgtaccc gagggtgtga gattctcaag cagccaggga gtgagctgac
     8701 cgatcagata agttcctatc cacagtgaaa tacctgctac cgacattgcc agtccgcgta
     8761 ccctggtagg gtacatttcc gacagcagaa caaatataac ggcacatatg gaaatggcac
     8821 agcaaaagat atacaggagg aagaatgcaa ggagaaacag ggatgccaat cctgcctttt
     8881 ccccgtacag aaaatacaga cctatcagga taagggaaat gatcattccc gacacgcccc
     8941 agtaaacaag cttcttccgg cccactctgt ctattataag gagtgcgatg acagttgttc
     9001 ccatgtttac gagtcctacc ataacctggt agaagagtga atcgccggtt gacaggccgc
     9061 ttttttcaaa tattgtcggc ccgtagtaca gaactgcatt tactcccatg aattgtccca
     9121 gcatggcaat tgccactccg atcagaacag ctttgaaaat gcctggcttc agcagcattg
     9181 tccattccga ttttgtttcc cggccggttg attcaagtat ggaatttatt tcctcttcgg
     9241 tatcctgcct gtttccgtga atacggtaaa tgatgctccg gcttttttca accctgtttt
     9301 tcaaaataag ccagcgcgga ctctcaggca cgaaaaaaat caggattatg aacaggaggg
     9361 caggaagggt ttccagtccg agcattgcac gccatacttc ggcattgaat attttgtgca
     9421 tcaggggact ggcaaaggaa ctcctgacgg agtaacccag aagcagataa ttgaacaggt
     9481 atgctgccag aaatcctatg gttattgcaa gctgatacag tgcaaccatg cttcccctgt
     9541 gtcttgccgc agatatttcc gaaatataga cgggagatac gatggatgcc actccaatac
     9601 ccatccctcc gattatcctg taaacaacca gtccgttgaa gccgcttgct attgcacagc
     9661 ccgcagcaga aacggtgaac agaacagctg aaatgaacat tgtgtttttc cttccgagat
     9721 aatcactcag tatgcctgca aaactgactc ctattattga acccacaagg gcacatccca
     9781 cataccagcc ggtctggatt gagtcgagac caaactgcag tgcaacctgt gaaattgtac
     9841 ctgagatcac tgcagtatca tatccgaaaa gaaagcctcc cattgcggct atcagggaaa
     9901 gaaaaaacgt atagatattc attgtttcaa aaagaattgt ttcatcaaga aagggcatat
     9961 atctgatacg ccagccgcca aacttacata aaaatcatgg cccgcaacaa aatcaccctg
    10021 tcttttcatt tattttccgt gtgactgaag gaaattcccc tgtctgctga aagaggcaaa
    10081 gggattttat gagagttatt gcctggtttt ttgccctgca ggtgaaatta atgatctgag
    10141 gcttctggtc atcctgctga tactgcgccc tgctttctgt acaagggata atttttcgga
    10201 caggatgaat tcattcagga tttccgtgaa ctgcgtagcc cgcagatatc cgaatgattt
    10261 atggggattg cggatcccgt tgctttcgta gttgttgatg atcagaatat caaagggttt
    10321 aacaccgtgg gcattctcag agtaatgatc tgataatctg taattaaatg aaaccttgtc
    10381 atgcagatca gaaaaattat accactttct gattacaccg ggaggcgtgg caagctgttt
    10441 attgtccatg ccccgttgtc cggcttcggc ggccgtcctg ctgatgataa ccggaagacc
    10501 gagcggggat cccattgtta caagggtatt gatcctgatg tcgggtgcaa gaaacgtcag
    10561 aacatcataa gcaatgatcg atcccattga atggccgatc agcattattt cgtccttgct
    10621 gtgtgccttc agaagagaga gcaggcgctc ccttatcagt tccccggcat taagcagtgc
    10681 attctttttt atatcaagaa tatgacctga atagtatatt tcaagatcgt taaaatacct
    10741 gtggattata gcgtcggtta tgaatgaata attcagagtc agatcttcat tgagaaatat
    10801 ccggttcatc tgacggccaa ggaaatcaat aacctttttc cgggtactgt aatcttcacc
    10861 tgtaaaatcc ccggaagctt tattgtattt ttcatccata taatacgggc tgaccggatc
    10921 tttttcggaa ggatcctgtc ttttgtcata tagaatatca gcccagtatg ctaggctgaa
    10981 tcttatcatg gcactatcat ggcctgaatt tttcagccct tcggccattg cagccttcca
    11041 ccaggttgcg agaatttcct ccggtggttt gttgcccagg ccatggatac ctattattac
    11101 atttgccatg atcccgcaaa attaatatta ataatcaccg gatgaatatg ttcttcgtta
    11161 cgaaatgtta actttaatgc aggagattct ttttaacggt ccgggtacag tagaggttga
    11221 aatgttttaa ttaatctttg aaacatgaag gaagaataca gattgggcag gctgttcggg
    11281 caggcactca tatatgccgg atatttcctt atcgtttccg gagtgataat ctcgtgtttc
    11341 agtattgccg gcctgctcct tgtactagcg ggaatatttg cagtattcac atatgaagga
    11401 accattatcg attttgagag gcgaaggctg aaaaactaca tctgcctttt cggatgggtt
    11461 aaagccggca ggtggtacag tgtggaccat ttcaggaaat ttacaattta taccaaagga
    11521 agaagatcaa cgggcaggag caggcggaat gccccctcaa accttacaga caggaacatc
    11581 aggctggccc tggtaagtag ggacggatcc agaaaaattg tcgttaacag atatgatact
    11641 ttcgatgctg cacggagaga aatgaatgag ctgataaaag atctgaaact caaggaagca
    11701 taaaccagtt acatctgtcc acccgccatt tgttctctcc ggcactgaag acagtgcgct
    11761 tatttaagtc ggagctgttt attttttaac ggattcctcg taaaaatcac agattccgtt
    11821 tttgttttca tctttaaaaa accttcctcc cggtgcaagt ccctgtccct gccccggccc
    11881 cagtccgcgg ccccagcctt ttgcgggacc cagtccacgg ccctgtccgg gagccattgc
    11941 acggccccat ccgccgcccg ggcctggtcc gccgc
//
LOCUS       DAOSPX010000066        11740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5437_length_11740_cov_4.239623, whole genome shotgun sequence.
ACCESSION   DAOSPX010000066 DAOSPX010000000
VERSION     DAOSPX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5437_length_11740_cov_4.239623"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="lpxB"
                     /locus_tag="PLZ75_04965"
     CDS             complement(<1..872)
                     /gene="lpxB"
                     /locus_tag="PLZ75_04965"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOS71782.1"
                     /translation="MKYFIVAGEASGDLHGSNLVKELFRQDRNAEIACWGGDLMQSAG
                     ARVLMHYRNTAFMGFMVILKNLGRVLRNISLCRKHVIDYNPDVLILIDYPGFNLRIAK
                     FAKRAGIRVFYYISPKFWAWGEKRVEKVRKRVDRMFIIFPFETEFYAKYGIPVKYRGN
                     PLVDEIESRIASLSGKEEISEMLQLSEKPVIGILPGSRRQEIRSILPQIMKVTDDFPG
                     YQFVIAGVRNVPAGLYRSITGNSRVRVVTDRTYELLSVCEAAIVKSGTSTLEAALFNI
                     PQVVCYRGDFFSML"
     gene            complement(996..1163)
                     /locus_tag="PLZ75_04970"
     CDS             complement(996..1163)
                     /locus_tag="PLZ75_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71783.1"
                     /translation="MTDSGIATQMITLCVVPNVLVFLIFNHYDMLKAARGVLGLTIAW
                     AFLIFAVKFLV"
     gene            complement(1166..1957)
                     /gene="surE"
                     /locus_tag="PLZ75_04975"
     CDS             complement(1166..1957)
                     /gene="surE"
                     /locus_tag="PLZ75_04975"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOS71784.1"
                     /translation="MIIEKEDKLILVTNDDGLYAAGLRTLLEVMEEFGKVVLISTLES
                     QSGMSQALTVKTPLRTKLLEENERHRIYACNGTPTDSIKLAINQLLERKPDFVVSGIN
                     HGANASVSVLYSGTMAAALEGCLYGITSVGFSLNSFSREIDFSVCRDFIRIIMNDLSA
                     GPLPEGICLNVNIPAVKREEIRGMLVCRQSRGNWKEEFDKRKDPMGRTYYWLTGLYNN
                     HEPDAEDTDEWALRNNYVSVVPVSIDMTAHGYIDTLRNRLNGSSR"
     gene            2196..3230
                     /locus_tag="PLZ75_04980"
     CDS             2196..3230
                     /locus_tag="PLZ75_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71785.1"
                     /translation="MRYLLWFVLSVFYPGVSGQTQGIYFKRVDEPREKALSLLIPSGW
                     TIDGGAIRIMSDQIAGAANMIDCKFDMAVKDDQTGSVMIRWLPEMMCVDQSRAFGYPE
                     GSVFNNCLVRRKRTPSGFIYEVAIPYAHPEAVDVNIISEKPLQELAELFHRLTDPSVL
                     AFTNLSYQAGMLEYSYSERGKKYQERMITVIEDYGYSGAGMWKNRSTMLIRAPFGELD
                     KWEPVLSVIQNSGKWNTSWVVNEANGQRQRAGQMILTQQEIQALDRAISESHNRTNSE
                     INKDMYLNLTGQNEFSNPHTGETETDTNHWKYRWVNNLGEIIYTDQQSYNPNFDPVLN
                     KSGFKLSTPR"
     gene            3419..5872
                     /gene="pheT"
                     /locus_tag="PLZ75_04985"
     CDS             3419..5872
                     /gene="pheT"
                     /locus_tag="PLZ75_04985"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOS71786.1"
                     /translation="MKISYNWLRDYLDIDIDPGKLSAILTAIGLEVEAVEEWESVKGG
                     LEGIVVGEVLTCKKHPGADKLTVTTVDTGTGGPLQIVCGAPNVAPGQKVPVATTGTRV
                     FKGDESIVIKKTRIRGELSEGNICAEDELGLGTSHEGVMVLDENAVPGTALSEYFEVV
                     RDTVFEIGLTPNRIDSGSHFGVARDIAAYLNINEDFSERAVLPSVENFRPDNNDNPYE
                     VIIENREACPRYTGITISGVKVGESPAWLKNRLRAIGQSPINNVVDITNFVHHEIGQP
                     LHAFDADKIDGRKVIIRNLPDNSPFITLDGVERKLSSRDLMITNVNEGMCIAGVFGGI
                     KSGVTANTRNVFLESAYFNPVTIRKTAKRHSLNTDASFRFERGADPERSGWALKRAIM
                     LIKDIAGGKISSDVVDVYPEKIKRRTVDVSYRNIARLIGKQIDSQIIKKILDLLDIRI
                     LKEDGDRLTLEIPLYRVDVHREADVVEEILRIYGYNNIEIDDHVNSTLYFPPKPDREK
                     LQNIISDMLTANGYAEIMCNSLNPASFYESGDFDRDRLVVLANPLSSDLNALRQSLLY
                     GGLSSVARNINRQNTDLKLYEFGNCYFHKKSGGAFPVVDDYSEKKCLDIFISGNRGKQ
                     RWNYKTSPTDFYNIKAAVEMVFTRLGLDPGNIGNSESDRKYFSESVTYLSGNKLIAEA
                     GKISGEYLEKFDIGQDVFYAHIEWDQLIRLISRHRILFRELPRYPWVRRDLALLLDKE
                     IPFSRIKEIAFKTEKQILREVDLFDVYESESLGHNKKSYAVSFILRDEMKTLTDRTID
                     KTMNNLIEVFKTELKASIR"
     gene            5872..6393
                     /locus_tag="PLZ75_04990"
     CDS             5872..6393
                     /locus_tag="PLZ75_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOS71787.1"
                     /translation="MEKMCKERIELIKGDITTLDVDAIVNAANNSLLGGGGVDRAIHK
                     AAGPELYTECLKLDGCRTGEAKITKGYNLKARNIIHTVGPIWHGGSRNETDLLASCYK
                     SSLALAKSNNIESIAFPGISTGAYRFPKDLAALIAVNETKRFLKNNSSPSRVIFVAFD
                     DESYDIYQKLLCQ"
     gene            6390..7262
                     /locus_tag="PLZ75_04995"
     CDS             6390..7262
                     /locus_tag="PLZ75_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOS71788.1"
                     /translation="MSVFFRSVRNYLSLVRFSHTVFAMPFALIGFTLAVTGDSEKFSF
                     RLFLLIILCMVLARNAAMGFNRIADRKYDALNPRTKDREIPSGKISPASAAIFVTVNS
                     VLFIAAAGFINRLTLMLAPLALLIILGYSLTKRFTSLCHFILGLGLSLAPIGAYISVT
                     GRFSFLPLVYSFIVLTWVSGFDIIYSLMDDSFDRYNRLHSIPSALGISKAIRVSVLVH
                     LLTIILVIVAGTAGNGRFLFWTGALIFISLLIYQHLIVKKDDLSRVNLAFGTTNGIAS
                     IVFAAFVILDLILL"
     gene            7460..8581
                     /locus_tag="PLZ75_05000"
     CDS             7460..8581
                     /locus_tag="PLZ75_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71789.1"
                     /translation="MAELLVREVLTKRDLRKFIYLPAEIHKNDPEWLPPIYMDEWELF
                     DSKRNRAFNYSDTALWLAFRGKKPVGRIMGIINRRYNEIHGENHGRFSFMECFEDQEV
                     FHALISTAEKWAREKGMEKLVGPLGFSDKDPQGFQIEGFEYPSFIVSATNSPYMPVML
                     EREGFEKKVDLVNYFIRIPEKLPEIYERAYTRISRNNQFRVIEFKSKQEIKKYILPVL
                     ELLNQTFIEIYGFVPLYDQEKMDLAKRYLMILDPQFVKVVESEGKMVGFAVGIPDISE
                     GIKAAKGKLFPFGFIRILKASRRSRKLLMMLGGIKKEFRNQGIDVLMAVKIIDSCIRH
                     KMDSIDVHLVLETNKTMIGECERIGGQILKKWRIYHKPL"
     gene            complement(8627..10537)
                     /locus_tag="PLZ75_05005"
     CDS             complement(8627..10537)
                     /locus_tag="PLZ75_05005"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOS71790.1"
                     /translation="MSLKNLKYLGLIFISLLAYNAYSQDKTQTDSLKRAMTDGQAVEM
                     ADSSGVETADSSGVELIDNPGKIVIDSLKRKGIILSDQKEMPEIVLSAEQAVKFLQER
                     SMPGYWKNPDDPLRAAIRQLVFKAINPPWDSAELFISKYPFDSLNVSWDKFYVWKPVR
                     MKIPAISPPPLPTVADTMALPVIPVWDYPPDTARIIDIKIPEFPERSVRRAYLKDTVI
                     YVVADTLREVVPDYPGFPFTHYDAPFQGDSLRTAVRTLSSYIQAKDSSIVYFTGLGDV
                     ITPVWLNSRSGMMHRYWLKTDLNDSVTVWIGSQDRNTIGLYLETGVSFARPVRQGYQS
                     TARIELQEIDKSTLLAVKKIITKPLRWKYHTETSFALNQAALSNWVKGGESNISTSID
                     ITGYADYTNKPYNITSNNFARIKYGLVASGDKGVRKNLDLLETNSKINHKAFGKFDFS
                     GILLFKTQIAKGYNYPNDSVPVSKFLNPGILTLGVGLDYKPDKNTSLNFSPVSYKGTY
                     VPDTANIDQTKYGIAANRRSKNEFGASFMISNVYKLTPTIALTNRLQLFTNYINNPQN
                     IDIDWEMIVTAKLNWFTDVRLNTHLIFDDDTKTAVYDKDNKPVMGPDNQIKKTARVQF
                     KQLLGFSFIFRF"
     gene            10792..>11740
                     /locus_tag="PLZ75_05010"
     CDS             10792..>11740
                     /locus_tag="PLZ75_05010"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOS71791.1"
                     /translation="MKLPLFEPYKIKMTEPVYTSSREEREKWIRDASFNVFNLKSEQV
                     TIDLVSDTGSGAMSDRQWSALFTGDESYAGTSSFDNLRQAIYDITGFQRIIPTYQGRI
                     ADSILFSAMLKEGDIVPCNSLFDTSRALIEYRKAIPVDCTIRESNDLYAYHPFKGNLD
                     LGRLDDILSNNSRERIPCIILSVTNNSTGGQPVSMENIRNIRELSYKYGVKLILNSSR
                     FAENAWFIKTREAGYADKSIREILLEMFSHADAMTMSAQEDAISNTGGFTAFRDETLY
                     HEAVRFNMVHEGLIHTGGMSGRDMNALARGLYEGTNTDFI"
BASE COUNT         3398 a         2648 c         2644 g         3050 t
ORIGIN      
        1 atcagcatcg agaaaaagtc ccccctgtag cagaccacct ggggaatgtt gaaaagtgcc
       61 gcttcaagag ttgaggtgcc cgacttcact attgcagcct cacacacact gagcagttca
      121 taggttctgt cagttactac cctcacccgt gaatttcccg taatgctccg gtacaggccg
      181 gccggcacgt tccttacccc tgctatcaca aactgatagc cgggaaaatc atcagtgacc
      241 ttcattatct gcggaagaat ggatctgatt tcctgtctgc ggctgccggg aagtattcct
      301 attaccggtt tttcagaaag ctgaagcatt tcagatattt cctcctttcc tgataatgag
      361 gcaatgcggc tttctatctc atccacgaga gggtttcccc tgtattttac agggatcccg
      421 tatttcgcat aaaactctgt ttcgaacggg aaaataataa acatcctgtc aaccctcttt
      481 ctcacctttt ccactctttt ctccccccag gcccagaatt tgggagaaat ataatagaaa
      541 acccttattc ccgctctttt tgcaaatttt gctatcctca ggttgaaacc cggataatcg
      601 atgagtatga gcacatcggg gttataatca atgacatgct tcctgcacag agatatgttc
      661 cggagcactc ttcccaggtt tttaagtatt accatgaagc ccatgaatgc cgtattcctg
      721 taatgcatca gcacccttgc cccggccgac tgcatcagat cgccacccca gcatgctatt
      781 tcggcattcc tgtcctgcct gaacagctct ttaaccagat ttgaaccgtg aagatctcct
      841 gaagcctcgc ctgctacaat aaaatatttc atcagtttag ttcattcgga aaattattat
      901 gacaacgctg taaaccgggc agcctgtatc cttcctgcgg cagaatatgc aggaccggct
      961 tcattgcttc cctccggtat tggcttgtga aagtttcata caaggaattt aaccgcaaag
     1021 atcaggaatg cccatgcaat tgtcaggcca aggactcccc ttgctgcctt cagcatgtca
     1081 taatgattga aaatcaaaaa aaccagtaca ttcggcacca cgcaaagagt aatcatctgc
     1141 gttgcaatac cgctgtctgt caaacttagc gggaagatcc attcagcctg ttccttagag
     1201 tatcaatata tccgtgggcc gtcatgtcaa ttgataccgg aacaaccgaa acatagttgt
     1261 tcctgagggc ccattcgtca gtatcctcag catccggttc atggttgttg tacaggccgg
     1321 tcagccagta atatgttctt cccatgggat ccttgcgctt gtcaaactcc tccttccagt
     1381 tgccccttga ctgccggcaa acaagcatcc cccggatttc ttcccttttt acagccggaa
     1441 tgttcacgtt gaggcaaatt ccttcaggaa gcggcccggc tgacaaatca ttcataataa
     1501 tgcggatgaa atccctgcac acggagaaat ctatctcacg tgagaagctg ttaagggaaa
     1561 agcccaccga tgtaatccca tacaggcagc cttcaagtgc tgcagccatg gtaccggaat
     1621 ataaaacact caccgatgca tttgcaccat gattgatgcc cgacacgaca aaatcgggtt
     1681 tcctttcaag aagctgattg attgccagct taatactgtc ggtcggagtg ccgttgcagg
     1741 catatattct gtgcctttcg ttctcttcaa gcagttttgt ccgcagcggt gtcttaacgg
     1801 ttagtgcctg tgacatcccc gactggcttt cgagggttga tataagaaca accttgccga
     1861 actcttccat aacctccaga agggttctca ggccggctgc atacagaccg tcatcatttg
     1921 taacaagaat cagcttgtct tctttttcta ttatcatata gcagactttg cctgcaaaga
     1981 taaggaaata ccggccttcc ctgcaataga ataaaggaaa cttaaatagt tgtacacttt
     2041 tacagcttta ctgaattgtg aaccggaaac agtcatgtgt gtcagacttg cggcaaggca
     2101 agaggctgtt acagaaagga tactgataat acttccataa ttattgatta ttctgtttcg
     2161 aattcataaa tttatattat aaaaaaaaga aagtaatgcg ttatttgctc tggtttgtat
     2221 taagtgtatt ttacccaggg gtttccggac aaacacaagg tatatatttc aagcgtgttg
     2281 atgagcccag ggagaaagcc ctcagcctcc tgattccttc aggatggaca atagatggcg
     2341 gcgcaatcag aataatgtct gaccagattg ccggtgcagc caatatgatt gactgtaaat
     2401 ttgatatggc tgtaaaggat gatcagacgg gaagtgtaat gatacgctgg cttccggaaa
     2461 tgatgtgcgt ggatcagtcc cgagccttcg gatatccgga aggatctgtc ttcaataatt
     2521 gccttgtaag aagaaaaaga actccgtcag gattcattta cgaggttgcg ataccctatg
     2581 cacatcctga agctgtggat gtaaatatta tttcagaaaa gccgcttcag gaacttgcgg
     2641 aattgtttca tagacttacg gatccttctg tacttgcatt tacgaattta tcgtaccagg
     2701 caggaatgct tgaatacagt tattccgaac ggggtaaaaa ataccaggaa agaatgatta
     2761 cagtgataga ggattacggg tacagcggag cgggtatgtg gaaaaacagg agcaccatgc
     2821 tgatcagagc gcccttcggt gaactggaca aatgggagcc ggtactgagc gtaatccaga
     2881 attcaggtaa atggaatact tcatgggttg tgaatgaggc aaatggtcag cgtcaacggg
     2941 ccggacagat gatccttacc cagcaggaga tccaggcgct ggacagggcg atcagtgaaa
     3001 gccacaaccg gaccaattcg gagatcaaca aagatatgta cctcaacctg acaggccaga
     3061 atgaattcag caaccctcac acaggggaaa ctgaaacaga tacaaaccac tggaaataca
     3121 gatgggtgaa taatctcggg gaaataatat atacagacca gcaaagctat aatccaaatt
     3181 ttgatcccgt actgaataag agcggattta aattatcaac acctagataa tcatcagaat
     3241 tacagaatcc ggcagtatat tccccgcttt gcttaatgcc ggaaccgagg atctcacatt
     3301 atttaattca attggccgca ccgagattct cacataaatt accggctgaa atagccggca
     3361 atgatcaaaa cattttgcaa aattcttact tttgcacttc tgttaataaa attacaaaat
     3421 gaagatctca tacaactggc tcagggatta tctcgacatt gacatagatc cggggaagct
     3481 gtctgccatt ctcacagcca tcggccttga agtggaagct gtcgaagaat gggaatctgt
     3541 aaaaggcggg ctggaaggca ttgttgtcgg ggaggtgctg acatgcaaaa aacatcccgg
     3601 agccgacaag cttacggtta caaccgttga taccggcaca ggagggcccc tgcagatagt
     3661 atgcggtgcg ccgaatgttg cacccggaca gaaagttccg gtagccacca caggtacccg
     3721 ggtttttaag ggtgatgaga gtattgtaat caagaaaaca aggataaggg gcgaactttc
     3781 cgaaggcaac atctgcgccg aggatgaact gggcctcgga acatcgcatg agggtgtcat
     3841 ggtactggat gagaatgccg tgccgggcac ggccctttca gaatatttcg aagtagtcag
     3901 ggatacagta tttgaaatag gcctgacacc aaaccggatc gacagcggct cccatttcgg
     3961 agtggccagg gatatcgcag cctatctgaa cataaatgaa gatttctcgg aaagagcggt
     4021 tcttccgtca gtggaaaact tcaggcctga taacaacgac aatccctatg aagtgataat
     4081 tgagaacagg gaagcatgcc ccagatacac cggtatcacc ataagcgggg tgaaagtggg
     4141 cgaatcaccg gcatggctca aaaacaggct ccgggctata ggacagagtc cgataaacaa
     4201 tgtggttgac atcaccaatt ttgtccatca tgaaattggc cagcctctgc atgcattcga
     4261 tgccgacaag attgacggaa gaaaagtgat catacgcaat cttcccgaca attcaccatt
     4321 catcacactc gacggtgtgg agagaaaact ctcttccaga gacctcatga tcaccaatgt
     4381 caatgaggga atgtgcattg caggtgtatt cgggggaata aaatcgggtg ttaccgcaaa
     4441 tacgaggaat gtctttttgg aaagtgcata tttcaatccg gtaaccataa ggaaaacagc
     4501 aaaaagacat agcctcaata ccgatgcatc attccgtttc gaaaggggtg ccgaccctga
     4561 aaggtccggc tgggccctca aaagagcaat aatgcttatt aaggatattg cagggggaaa
     4621 aatatcatcg gatgtggtgg atgtttatcc ggaaaagatt aaacgcagga ctgttgatgt
     4681 aagctacaga aacattgcca ggctgatcgg gaaacagatc gattcacaga tcattaaaaa
     4741 aatactggat ctgctggata tcaggatact gaaagaggac ggcgacaggc tgacccttga
     4801 gatacccctg taccgggttg atgtccaccg tgaagccgac gtggtggaag agatactgag
     4861 aatatacgga tataataata ttgagattga cgaccatgtc aactcaacac tttatttccc
     4921 tcccaaaccc gacagggaaa agcttcagaa tatcatctcc gatatgctga cagccaacgg
     4981 atatgcagaa ataatgtgca attccctcaa tccggcatcc ttttacgaaa gcggtgattt
     5041 cgaccgcgac cggctggtgg tactggcaaa tcccctcagt tcagatctca atgcactgag
     5101 acagtccctt ctgtacgggg ggctcagctc ggtggcacgg aacataaacc gccagaacac
     5161 cgatcttaag ctatacgaat tcgggaactg ctatttccac aaaaaatccg gcggagcttt
     5221 ccctgttgtg gatgattact ctgaaaaaaa atgtcttgat atttttattt caggaaacag
     5281 gggaaagcag agatggaatt acaagacctc cccaaccgat ttctataata tcaaggcagc
     5341 ggttgaaatg gttttcacaa gactcggtct ggatcccgga aacatcggca attccgaaag
     5401 cgacagaaaa tatttctccg aatcagtaac ttacctgtcc ggcaataaac tcattgccga
     5461 agcaggaaaa atttccgggg agtacctgga aaaattcgat atcggccagg atgtgtttta
     5521 cgctcatata gagtgggatc agctcatccg gctgatcagc agacacagga tcctgttcag
     5581 ggagcttccc agatacccct gggtaagaag ggatcttgcg cttctcctcg acaaggaaat
     5641 ccctttcagc cggataaagg agatagcttt taaaacggaa aagcaaattc tcagggaagt
     5701 ggacctcttt gatgtatacg agagcgaatc gcttggccat aacaagaaat cctatgcagt
     5761 gagcttcatt ctgagggatg aaatgaaaac actcactgac aggaccattg ataaaaccat
     5821 gaataatctg atcgaggtgt ttaaaacaga acttaaagca agtataagat aatggaaaag
     5881 atgtgtaagg agagaattga actcataaaa ggagatatta ccactcttga cgtcgatgca
     5941 atagtgaatg ctgcaaacaa ctcacttctg gggggcggcg gggttgacag ggccatacac
     6001 aaggctgcag gacctgaact ctataccgaa tgtctgaaac ttgacggatg ccggacgggc
     6061 gaagcaaaaa tcacgaaggg ttacaatctg aaagccagaa atattattca cacagtagga
     6121 cctatctggc atggaggcag ccggaatgaa accgatttgc tggcttcatg ctacaagtca
     6181 agccttgcac tggccaaatc caataatatt gaatcgatag ccttcccggg aatatccaca
     6241 ggtgcataca ggtttccgaa agatcttgca gccctgattg cagtcaatga aacaaagaga
     6301 ttcctgaaaa ataattcctc cccctcgaga gttatattcg ttgcattcga tgatgaaagc
     6361 tacgatatat accagaagct tctctgtcaa tgagtgtttt tttcaggtcg gtaaggaatt
     6421 acctgtctct tgtcaggttc agccatactg tgtttgccat gccttttgcc ctgatcggct
     6481 ttacactggc agtgaccgga gacagtgaga aattcagttt caggcttttt ctgctgataa
     6541 tactctgcat ggttctcgca cggaatgctg ccatgggttt taacaggatt gccgacagga
     6601 agtatgatgc actgaatccc aggacaaaag accgggaaat tccttctgga aaaatcagtc
     6661 cggcatcagc tgccattttt gtgacagtaa attccgtact gtttatcgct gcagccggat
     6721 ttatcaacag gctcaccctg atgcttgcac ccctggcact gctgattatt ctcggttaca
     6781 gtcttacaaa acgctttacc tcactctgtc atttcatact cggcctcgga ctgagcctgg
     6841 ctcccatcgg ggcatatatt tccgtcaccg gccgcttttc atttctgccc cttgtatatt
     6901 cattcatcgt actgacatgg gtgagcggct ttgacatcat ctattcgctg atggatgatt
     6961 cctttgacag gtataacagg ctgcactcaa taccctcagc ccttggcatc agcaaagcaa
     7021 taagggtatc agtgcttgtt cacctgctga caattatcct cgtcatcgtg gcaggaactg
     7081 caggaaacgg ccgctttctt ttctggacgg gtgccctgat attcatctcc ctgctaatat
     7141 atcagcatct cattgtgaag aaagatgacc tgagcagggt aaacctggcc ttcggaacca
     7201 ctaacggcat cgcaagcatt gtctttgccg cctttgtcat actcgatctg atcctgcttt
     7261 gaacataatt ccgtaaacac ctgcagcagc ctgcaatcat tattcccttg ccctgatttt
     7321 tcctgttgcc cggacgacat cccctgtttt tccggcacct cagaagaata aaagcataaa
     7381 aaagataaaa gaatgaaaca cagatcagtt tttttcatat acttgtttta ttttaacctg
     7441 cattgtaaac tgaactaaca tggctgaact gcttgtcaga gaggttctga caaaaagaga
     7501 cctcaggaaa ttcatttatc ttcccgctga aatccacaaa aatgatcctg aatggctgcc
     7561 acccatctat atggatgagt gggaattatt tgacagtaaa agaaacaggg cattcaatta
     7621 ttcggatact gccctctggc tggctttcag gggaaagaag ccggtgggca ggataatggg
     7681 aataattaac aggcgttaca acgagataca cggggagaat catggccgtt tcagcttcat
     7741 ggaatgcttt gaagatcagg aagtatttca tgcactgatc tccacggccg agaaatgggc
     7801 cagggaaaaa ggaatggaaa agctcgtggg accactggga ttctccgaca aggatcccca
     7861 gggtttccag attgagggat tcgaatatcc ttccttcatt gtcagtgcca caaactcccc
     7921 ctatatgcct gttatgctgg aaagggaagg ctttgaaaag aaagttgacc tggtaaacta
     7981 cttcatcagg atccctgaaa aactcccgga gatatatgaa agagcctaca cgagaatcag
     8041 cagaaacaat cagttcagag taatcgaatt caaatcgaag caggagataa aaaaatatat
     8101 cctgcctgtc ctggaactcc tgaaccagac tttcatagag atatacggct ttgttccgct
     8161 ttatgaccag gaaaagatgg accttgcaaa aagatatctc atgatacttg accctcaatt
     8221 tgtaaaagtt gtggaatctg aaggaaagat ggtgggcttc gccgttggca taccggatat
     8281 cagtgagggt ataaaggcag caaaaggaaa attgtttcca ttcggtttca tcaggattct
     8341 gaaagcctca agaaggtcaa gaaagctcct tatgatgctg ggcggcataa agaaggaatt
     8401 caggaatcag ggaatcgacg tgctgatggc agttaaaata atagattcat gcatacggca
     8461 caaaatggat tccattgatg tccacctggt tctggaaacc aataaaacca tgataggcga
     8521 atgtgagaga ataggcggac agatactcaa gaaatggagg atataccaca aaccgctcta
     8581 aaacctgaaa aagatcaaaa tctgaacaaa ttaaagcctc aacagattaa aacctgaata
     8641 taaaggaaaa acccagtaac tgcttgaact ggacccgggc agttttctta atctgattat
     8701 ccggtcccat caccggcttg ttatccttgt cataaacagc tgtctttgta tcatcatcaa
     8761 atataagatg ggtgttgagc cttacatcgg taaaccagtt gagtttggcc gtaactatca
     8821 tttcccagtc tatatcaata ttctgcggat tattaatata attggtaaag agctgaagcc
     8881 tgtttgtcag cgcaatggtt ggagtcagtt tatatacatt gcttatcatg aagctggccc
     8941 cgaactcatt ctttgatctc cggtttgctg caataccgta tttagtctgg tcaatatttg
     9001 ccgtatccgg cacataagta cccttgtagg ataccggtga aaagttgagg gaagtatttt
     9061 tatccggttt ataatcaagt cccacaccga gtgtaagaat tcccgggtta agaaatttcg
     9121 acaccggcac cgaatcattg ggataattat atcccttggc tatctgcgtt ttaaacaaaa
     9181 gtatcccgct gaaatcaaac ttcccgaaag ccttgtggtt gatcttggaa ttggtttcca
     9241 gcaggtcgag gttctttctt acccctttat cgcccgaagc aaccagacca tacttgatac
     9301 gggcaaaatt attggatgtg atgttgtatg gcttgtttgt atagtcggca tacccggtaa
     9361 tatcaatgga tgttgatata ttgctttccc ctccctttac ccagtttgaa agtgcagcct
     9421 ggttcagggc aaatgaagtt tcggtgtgat acttccagcg gagaggtttt gtgatgattt
     9481 tttttactgc aagcaatgtg gatttgtcta tctcctgcaa ttcgattctt gctgttgact
     9541 gataaccctg tctcacgggc ctggcaaaac tcacgcccgt ctcaaggtaa agaccaatgg
     9601 tatttctgtc ctgtgagcca atccataccg ttaccgaatc attcagatca gtcttcagcc
     9661 agtaccggtg catcatccct gaacgggaat tgagccagac cggagttata acatccccga
     9721 gaccagtgaa atagactatg cttgaatcct ttgcctgaat gtaactgctg agtgttctta
     9781 ctgctgtcct tagagaatca ccctggaatg gtgcatcgta atgcgtaaac gggaatccgg
     9841 gataatccgg aaccacttcc cgcaaagtat ctgcaaccac gtatatgaca gtatccttta
     9901 aatatgccct gcgtactgat cgttccggaa actcagggat tttaatatca atgatccggg
     9961 ccgtgtcggg gggataatcc cagaccggga ttactggcag tgccatcgta tcggcaactg
    10021 tgggcaaagg agggggagag atggcaggaa tcttcattct gacgggcttc cagacataaa
    10081 atttatccca cgaaacattc agggagtcaa aagggtattt tgagatgaac agttccgcgg
    10141 aatcccaggg aggattgatg gctttgaata ccagctgacg gattgcagcc ctcagaggat
    10201 catcaggatt tttccagtat cccggcatgc tgcgttcctg aaggaatttt actgcctgtt
    10261 cagcagaaag tactatttcc ggcatttctt tctggtcact gagtattatt cccttccttt
    10321 tcagcgaatc aatcacaatc tttccgggat tgtcgatcag ctccacgccg gagctgtcgg
    10381 ctgtttccac tccggaactg tcagccattt ccactgcctg accgtctgtc attgcccttt
    10441 tcagactgtc agtctgtgtt ttatcctgag aataagcatt ataggcaagt aatgaaataa
    10501 atattaatcc cagatatttt aaatttttta aactcattat aattaaaact ctaatttttt
    10561 tcgttgaata tattataaaa tatttataat tttacctgtc tgatgatccc agaaaaatgg
    10621 acttttctta taacggtaaa ttaccttccg gatcataagg caaacagata acggtaattt
    10681 aaccggttat attttataag ggaattcctc ataattatac aaatccaata gctggctgca
    10741 ggggagtatt ttgactctac ggttagttat tattttgtat catctgtaat tatgaaatta
    10801 ccattatttg agccctacaa gataaaaatg acggagcctg tatacacaag ctccagagaa
    10861 gaaagggaaa aatggataag ggatgcttca ttcaacgttt tcaatctgaa atccgaacag
    10921 gtaaccattg atcttgtctc tgataccgga tccggtgcca tgagcgacag gcaatggtca
    10981 gccctgttca caggagatga aagttatgcc ggcacctcat catttgataa cctccggcag
    11041 gcaatctacg acattaccgg attccaaagg atcataccaa cctaccaggg ccggattgcc
    11101 gacagtattc ttttttccgc aatgcttaaa gagggcgata ttgtcccctg caattcactt
    11161 ttcgacacat caagggcact cattgagtac aggaaggcaa ttccggtcga ctgcaccatc
    11221 agggaatcaa atgatcttta tgcatatcat ccatttaagg gaaacctgga cctgggaagg
    11281 ctggatgaca ttctcagcaa caattccagg gaaagaattc cctgtatcat cctgtctgtt
    11341 accaacaatt cgaccggagg ccagccggta agcatggaaa atatcagaaa tatccgtgaa
    11401 ctatcatata aatatggtgt aaaactaatc ctcaactcgt cgaggtttgc cgaaaatgcc
    11461 tggttcatca aaaccaggga agccgggtat gccgataaaa gtatcaggga gattctgctt
    11521 gaaatgtttt cccatgcgga tgcaatgaca atgagcgcac aggaagatgc catctccaac
    11581 actggcggat ttaccgcatt cagggatgag accttgtatc atgaagctgt caggttcaac
    11641 atggtacatg aaggactgat ccacaccggg ggaatgagcg ggcgcgacat gaatgccctg
    11701 gcacgcggac tgtatgaagg cacgaataca gatttcatca
//
LOCUS       DAOSPX010000067        11616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5507_length_11616_cov_4.562062, whole genome shotgun sequence.
ACCESSION   DAOSPX010000067 DAOSPX010000000
VERSION     DAOSPX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5507_length_11616_cov_4.562062"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PLZ75_05015"
     CDS             <1..636
                     /locus_tag="PLZ75_05015"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOS71792.1"
                     /translation="ADRTPQINAVWGKKFWNRAEKTSLKNYMGDYPSHFPKTQVRVKY
                     DENYIYVTFRVKDNYVKAVATQTNGRVFQDSCVEFFFTPGPDVSRGYFNLETNCKGVF
                     LFQYKNSNNKQAFVALDDARQIEISHSLEENVENEITKPLTWIIEYRIPFSVLSKYME
                     VDIPGPGVQWRANFYKCADMTSHPHWLTWAPVDFLKPSFHRPEFFGWLNFE"
     gene            775..2067
                     /locus_tag="PLZ75_05020"
     CDS             775..2067
                     /locus_tag="PLZ75_05020"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOS71793.1"
                     /translation="MLSLTLLASFCLLFPAFLIWLTKKYSFFKKIGAIVLAYATGILI
                     ANIGILPKSSDAYQQATIGQDRAYIPKTELHEMAEAGILSRSDVRYNGIAMIQDSMQS
                     ALVLLAIPLILFSLNVRRWLRFSGKGFLSMLLALISIITVVVTGYLIFRDRVEEAEKI
                     GGMLVGLYTGGSVNLASIALALNVDPHAFVMTNTYDMIVGALLIFFFITAGPAFFRLF
                     LPPFEAPVSTRVQKAAADDIDRQLTEDFDDYSGIFSRDVIIPLLRAIGLSLIILAMGL
                     AVSFLFPTVPMTISVILTITTLGVAASFIRPVREIKKTFQLGMYFIVAFSIVIATRCD
                     LSIIFQAKYLNLLYFVSYVVFGSLFLHLLLSWIFRVPAEDYMITTTGFVYSPPFVPMV
                     AAALKNRDIILTGLATGMIGWIIGNYIGVAFGMWLDKL"
     gene            complement(2083..3621)
                     /locus_tag="PLZ75_05025"
     CDS             complement(2083..3621)
                     /locus_tag="PLZ75_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOS71794.1"
                     /translation="MLSLLMLSGNSQQSKVIDRIIEIGRTDNRTMHHLDVITNRFGGR
                     PVGSDAYTNAAQWCASEFRKWGMEVIMDEAGSVPVGFNRGPWFGRLLSENGMTLHFAT
                     PSYTSGTKGVQRGHVLIEPKTRDEFERMKGALKGAWVLIGGKNDGWPIDYSEKADSAR
                     EKVIVYNDSIGKINRAIMFENFANRGKPPKELHPLKEEPALFYREMREAGILGVIQSS
                     AVPIRALYDRKNLDRMTFENLPTVCDIKLDEDQYAIIEKMAKERRYFLLEFDIRNHFK
                     PGPVKYHNVIGVIRGTRYPDEYVMMGGHLDAFDVATGGVDDGSGATPVMEAARLIMAA
                     GGRPQRTILACLWAAEEFGLLGSKHWVETNREKLPRISNYFNRDGGPTVASGIVVPDA
                     MYNDFEKVCKPLNSINPDFPFRLTRNTNPPRPRPTTASSSDHAYFAMNGVPTLSFETP
                     DSKGYNFDYGEIWHTERDTYDKSIPEYQEHTSVVTAVVVYGLANLDHLLSRKGLYLEP
                     EEKK"
     gene            3980..4879
                     /locus_tag="PLZ75_05030"
     CDS             3980..4879
                     /locus_tag="PLZ75_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOS71795.1"
                     /translation="MILKHQAGNLIPGENFSFINRILKGFGQIMLQENALTGLLFLAG
                     IFLGSLTMGVAALLAAVSGTVTAIVLKYDRENTDKGLYGFSASLTGVALMLFFKPAPA
                     VWLFVICGSVMAAMIQHFFIKRNMAVYTLPFVLVTWLIYLFLRYGFPGLLVPAASADP
                     VYSHFLFPLRGFGQVIFQEKVFSGLLFFTAVFISSPLAAVYGLAGALLSGILSQYLAV
                     EPESISRGLLSFNAVLCAILFSGNQFRNAAGALVSVLLSLIISVTMLRYNLIQLTFPF
                     VAASVITLFIGKVYQTVRSGSNR"
     gene            complement(4975..6435)
                     /locus_tag="PLZ75_05035"
     CDS             complement(4975..6435)
                     /locus_tag="PLZ75_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71796.1"
                     /translation="MKITAFIRSLAAFIFLLTISSSDYKILSQEIPDNIRILLEKLSP
                     LSYERGNRELIYQYSIGDLSRLSDKDTELVIKELSKRGIGVITFWKQAEAFERGIEEG
                     IRIARLQENLGLKVAVDATDLLYGFYDKTDFTAHIDNEGRTFYDSSFAGHWMGCPFTL
                     QERIPVIRSRIIAYSEAYQAAGVNIDIVTADWEIDGPHEWNEAWVNSKKCMTCCKNID
                     NIQDFYVFQSKLRSLRSKLLEICYTRPILERHPKALITNYGTYPNDGWRYWYDYFESP
                     QIDLPNIKDQKAIYRPWYNEFTETGFTMAMPVVYTWYPIYNSYPEYTSDYRWFYNMLK
                     VGTNAGKSTPSEIPIATFFHWHTTAPPEKQDLNVKQMSRHSYQELLWHLLLRGHDIFF
                     SWCRKEEIETEMTLLQEVYNNSLNYNDWLQSGRPVLFEVPAKESSVVSGVKLNNRVLV
                     RRTDFTDNKKAVKCIIDDKIIMVPYKPGECQIITIE"
     gene            complement(6527..8242)
                     /locus_tag="PLZ75_05040"
     CDS             complement(6527..8242)
                     /locus_tag="PLZ75_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS71797.1"
                     /translation="MKLTVISSLERIGQDDQLFGELQVKIKAAKNEYESFQVVVGAIL
                     KNIRVVNAEISDLSGRAGIFSKENFTLYRPEYIRISRPSARAQLPPGLYTDPLVPFIN
                     PQTGKPIEPRTQVREYWPGPVVSRGYEMYALPFDVWKGQNQPIWVDVFVPENTVAGEY
                     EGTFTVTLDNFPDPWGPVADSIRVKTISVPVSITIWDFTLPDQTTHRNHFGRVGRISE
                     RFGVEVNSDEYKKIEMEYCRIYTEHRLNPPFPHYLMPEMSTDGSLKIIPERHNELKKF
                     IEDFNVSDFEIPRAPIAGFTDPHKALNASDREKAVRYYSDFYKYLKENGWDKRAYVYL
                     YDEPNTPECYKRVLELAEDVHKGAPQLKILVVEQPYSHDPSWPDIDSAVDIWCPLFGF
                     IDRESVTDAISRGDEVWSYTALSQRAPRYHPHYDEVKNYDSPYWHIDAPLTSHRVPTW
                     MNYQYGIKGILYWSLTTTVMDTWYHPAFSHFGTHFNGGGYFIYPGVPCGIVGPISSIR
                     LKNLRESMEDYEYLNLYEKLAGREAVLKIVSKVAPNWWETTDNPNDIFAAREMLASEI
                     LKLTK"
     gene            complement(8597..9799)
                     /locus_tag="PLZ75_05045"
     CDS             complement(8597..9799)
                     /locus_tag="PLZ75_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71798.1"
                     /translation="MKRKFKMNKARSYRLLIYPLFWMVIYISVTSCGQEGSVNRITTD
                     EAFRIFFYSDSGGITGADGLFSLLLPDGSSLFMLGDCFLGKVVNGTRDPNTTMLRNAF
                     VVIDKNRTSAEPVYRGSWENPLTLMEPVNEPGDSTYRWYWPGHGFVRKDTLYLFALGL
                     VNIPDTAGTTDPAGQNGSTSPDSEPENIFTFAVTHVDLLSFSLPGFRHLETRRADIDY
                     PANLIDFGNCVLEDEDYIYIYGTKNLPFHAQIHVARVPAGSSRFYRDWEYYTGEGWDK
                     DIGKSEPMDIDISVSEQFSIFRYRNKYILLTQERAGNDIYTYISDLPYTGFHNKKFIY
                     HTPEPDKDPNQHIITYNALAHRQYIENDRLLVSYCVNSLNIRDIFDDVEAYRARFLRV
                     PLKYILKE"
     gene            complement(9802..>11616)
                     /locus_tag="PLZ75_05050"
     CDS             complement(9802..>11616)
                     /locus_tag="PLZ75_05050"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOS71799.1"
                     /translation="YGCLPPGPGTIAVNQLLTLCRPDMADNYVKSMLHLHSHGGWFPD
                     NSEGIGYPGLSASSHVIPLLAAARQKGIHDYDIQDLYRAVQHDLTSPGQAHAGGGYSG
                     RKYLEEYLLSDSVTPAGPPACIIREYAYHDWVMSQMARASGNVSDYMTFARRAFKYKN
                     VSGPEEDVMQNFVPHDAAGLITVYGKEEFTVRLEKEISRHLAHAGQEEAIADLLKKML
                     YSGDCPPVQSPLQAAYLFNFAGAPWLAQKWLREILSGYGEHHSPLSSVLCYPEQSAAL
                     YALGAIGLFQTDGGTSARPCYEIGSPLFDRITIRLDGKYYPGGKFVIEAGNNSSGNIY
                     VRKAALNGKELTGPWFPHTDLSSGGNLVLRMGSRPDKTWGIRPENAPPSMSSGMTEAE
                     IGEIMKYDRFAEDLELWNKAMKAYYYHRKEHFETLPNADDEIIFLGNSITDQAEWSEM
                     FGNPRIKNRGIGGDVTDGILERLDEVTESRPLKIFLMIGTNDLSEGRSVDYITENFKR
                     IIERIRESTPATKIYIQSVLPVDDAVHYTRRNSDIIAVNDRLKEIASLNGLEYIDLFS
                     LFRLENNRLNPEYSYDGLHLNGKGYQVWKEAIRKYVEE"
BASE COUNT         3073 a         2694 c         2456 g         3393 t
ORIGIN      
        1 gcagatcgca caccgcagat aaatgcagtg tggggcaaga aattctggaa cagggcagaa
       61 aagacaagtc tgaagaatta tatgggagat tatccgtctc atttcccaaa aacccaggta
      121 agagtgaaat atgatgagaa ttatatttat gtgacttttc gcgtaaaaga caactatgtc
      181 aaagcagtgg ccacccaaac caatgggagg gtgtttcagg acagctgtgt tgaattcttt
      241 ttcacccccg gtcctgatgt gagcagagga tatttcaacc tggaaacaaa ctgtaagggt
      301 gtttttctgt ttcagtacaa gaacagcaat aacaaacagg cctttgtcgc ccttgatgat
      361 gcccggcaga tagagatctc gcattccctg gaagaaaatg ttgaaaatga aatcacaaag
      421 cctctgacct ggatcattga ataccgtatt cccttttctg ttctttcaaa atatatggaa
      481 gtggatattc ccggacccgg agttcagtgg agagccaatt tttacaaatg tgctgatatg
      541 acttcccatc cgcactggct cacatgggca cctgtcgatt ttctaaagcc tagttttcac
      601 cgtccggaat tctttggctg gctgaatttt gaataaaagg ctcaagggag agtcccggaa
      661 ctgtttatct gggtaaaaac accaccggca aggtggttaa aatacctctt ttcaggatat
      721 gggagcccaa aggctaaggc atttgtatat ttttgatata ctaaactcat tgctatgctt
      781 tcactgacac tcctggcaag cttctgcctt cttttccctg catttcttat ctggcttaca
      841 aagaaatatt cgttttttaa aaagatagga gccattgtgc tggcctatgc cacggggatt
      901 cttattgcaa atatcggcat actgccgaaa agcagcgatg cctaccagca ggccaccata
      961 ggtcaggacc gggcatacat cccgaagact gagctccatg agatggcaga agcaggaata
     1021 ctgtcaagaa gtgatgtgag atacaacggt attgccatga ttcaggacag tatgcagagt
     1081 gccctggttc tgcttgccat acctcttata ctcttttccc tgaatgtcag aagatggctt
     1141 agattctcag gcaagggatt tctctccatg ctcctggccc tgatttcaat tattacagtc
     1201 gttgtcacgg gttatctgat cttcagggac agggttgagg aggcggaaaa aataggagga
     1261 atgctggtcg gtctttatac cggaggatca gtaaacctgg cctcaatagc acttgcactt
     1321 aacgttgatc cgcatgcctt tgtcatgacc aatacttacg acatgatagt cggtgccctg
     1381 ttgatattct tcttcatcac tgccgggccg gcctttttca gattattcct gccgcccttc
     1441 gaagcgcctg taagcacaag ggttcagaag gcagcagctg atgatataga caggcagctg
     1501 acagaagatt ttgacgacta ttcagggata ttcagccggg atgtcattat accactgctg
     1561 agagccatcg gactgtccct gattatcctg gcaatgggac ttgccgtcag cttccttttt
     1621 cccacagtgc cgatgacaat atcggtgata ctgaccatta ccacactggg cgtggcagcc
     1681 tctttcatca gaccggtacg ggaaatcaaa aagacctttc agcttggtat gtacttcatt
     1741 gtggctttct ccattgtcat tgccaccaga tgcgacctgt ccattatttt ccaggcgaaa
     1801 taccttaatc tcctgtattt tgtaagctat gttgtatttg gctccctctt cctgcatctc
     1861 cttctttcat ggatattcag ggttcctgcc gaagactaca tgattaccac caccggtttt
     1921 gtgtattcac caccctttgt accgatggtg gctgcagccc tgaaaaacag ggatatcatc
     1981 ctcaccggac ttgccaccgg tatgatagga tggatcatag gcaactatat aggtgtagct
     2041 ttcggcatgt ggctcgacaa gctttaacag gtactataca gtttatttct tctcttccgg
     2101 ctccaggtaa agtcctttcc ttgacagcag atgatcgagg ttggccagtc cgtagaccac
     2161 cacggccgtc accaccgaag tatgttcctg gtattccgga atgctcttgt catatgtatc
     2221 cctctcggta tgccagattt ccccgtaatc gaaattatag ccctttgagt cgggggtttc
     2281 aaacgaaagg gtcgggactc cgttcatggc aaaataggca tggtcggaac tgcttgccgt
     2341 tgtcggcctc ggacggggcg gatttgtatt ccttgtcagt ctgaaaggaa aatcaggatt
     2401 aatactgttc aggggcttgc atactttttc aaaatcattg tacattgcgt cagggacaac
     2461 gattccggat gcaacggttg gtccgccgtc acggttaaaa taattggaga tcctgggaag
     2521 cttttccctg tttgtctcca cccagtgttt ggatcccagc aatccgaatt cctctgccgc
     2581 ccacaggcag gcaagtattg tcctttgcgg cctgccgccg gctgccatta tcagcctggc
     2641 tgcttccatg acgggcgtgg ctccggatcc gtcatccaca ccaccggttg caacatcaaa
     2701 tgcatccaga tgtcctccca tcatgacata ttcgtcggga tagcgcgtac ccctgatgac
     2761 tcctataaca ttatgatatt taaccggacc cggcttgaag tgattgcgga tatcaaactc
     2821 gagaagaaaa taacggcgtt cttttgccat tttttctatt atggcatact gatcttcatc
     2881 aagcttaata tcacaaactg tcggcagatt ttcaaatgtc atcctgtcaa ggtttttcct
     2941 atcgtacaag gctcttatag gtaccgctga tgactgaatc acaccgagaa tgccggcttc
     3001 ccgcatctcg cggtagaaaa gggccggttc ttcctttaag ggatgcaatt ctttcggggg
     3061 cttgccgcga tttgcaaaat tttcaaacat tattgcccgg ttgattttac ctatcgaatc
     3121 attgtatact atgacttttt cacgcgcaga atcagctttt tcggaataat ctatcggcca
     3181 gccgtcattc ttgccgccga taagtaccca tgctcctttc agtgctccct tcatacgttc
     3241 aaactcatca cgggttttag gctcaataag aacatgcccc ctctgaacac cttttgttcc
     3301 cgaggtatat gagggagttg caaaatgcag tgtcatgccg ttttccgaaa gcagcctgcc
     3361 aaaccagggc cccctgttga atcccaccgg aacactgccg gcctcatcca ttataacttc
     3421 catgccccat ttccggaatt cggaagcgca ccactgggct gcattcgtat aggcatctga
     3481 accgacaggc ctgcctccga atctgtttgt gataacatca agatggtgca tggtccggtt
     3541 gtctgtcctg ccaatttcaa taattctgtc aataaccttc gattgctgcg aattaccgct
     3601 taacatcagc aatgaaagca tccagaatac aaatagtttt ttgatcattt tatcgaattt
     3661 tagttatcat ggtcacaatt ttagcaataa aatcaaataa atactattca ggaagggtaa
     3721 ttatcctcct gaaaaagcca gattcccgga acggaattgt tccggaaaag ccgtgctcta
     3781 tttccggtat tataccagta tgaccgggcc agcttatcat ggtttgaaaa agaaacaagg
     3841 atgaaaaaaa taatattgta acggcaaact gaaatacagt aaatttggac cggtattaaa
     3901 caaaaagctg ccagagaaca cggaatataa gcgggaccac agactttttt tcataaaacc
     3961 agataaaaac aattgtaata tgatattgaa acatcaggca ggaaacctga ttcccggtga
     4021 aaatttctcc tttataaacc ggattttaaa aggattcgga caaatcatgc ttcaggaaaa
     4081 tgcattaacc ggattgctgt ttctggccgg tattttcctg ggatctctca ctatgggggt
     4141 ggcagccctc ctggctgcag tcagcggaac agtcacggcc attgttttaa agtacgaccg
     4201 ggaaaatacc gataagggac tgtatggatt cagtgcttca cttacgggcg ttgcactgat
     4261 gctgtttttc aaacctgctc cggcagtgtg gctgtttgtc atatgcgggt ccgttatggc
     4321 agccatgatc cagcattttt ttataaaaag aaacatggcc gtatatacac tgccatttgt
     4381 actggtcacc tggctgatat acctgtttct cagatacggc ttccccggct tactggtgcc
     4441 agcggcatct gctgatcccg tttacagtca ttttttattt ccgctgagag gtttcggaca
     4501 ggttattttc caggagaagg tattttcagg tctccttttt ttcacagcag tgttcatcag
     4561 ttctcctctg gcagcagtgt acggacttgc aggagcattg ttgtcgggaa ttctttcaca
     4621 ataccttgca gttgaaccgg aaagcataag caggggattg ctgagcttca atgccgtatt
     4681 atgcgccata ctcttttccg gtaatcagtt caggaatgca gccggagccc ttgtttcggt
     4741 gctgctgtcc ctaataatca gtgttacaat gctaagatac aatctgatac agctcacatt
     4801 cccctttgtt gccgcttcgg taatcacact gtttatcggc aaagtttatc aaaccgtccg
     4861 gtccggttca aacaggtagt gttccaatca gcgtgtccgt ggcagactgt caaaaggtac
     4921 tgaagatgat acaattaata caatgagttc ttaagaccgg actgaaatag cctgttattc
     4981 aatcgttatt atctggcatt ctcctggttt gtacggaacc ataataattt tatcatcaat
     5041 aatacatttg actgcttttt tattatctgt aaaatcagtt cttcttacca atacccggtt
     5101 attcaacttt acaccggaaa caacactgct ttcctttgcc ggtacctcaa acaacaccgg
     5161 acgtcccgac tgaagccaat cattatagtt aagggaatta ttatatactt cctgtaacaa
     5221 ggtcatttca gtctcaattt cttctttcct gcaccaggaa aaaaatatat catgccctct
     5281 cagaagcaga tgccaaagta attcctgata agaatgcctg ctcatttgtt taacatttaa
     5341 atcctgcttt tcagggggtg ctgttgtatg ccagtgaaaa aacgtagcta tcggaatttc
     5401 agaaggagtt gatttacctg cattggtacc aactttaagc atattataaa accagcggta
     5461 atcagaagtg tattccggat atgaattata tatcggatac caggtataaa caacgggcat
     5521 ggccatcgta aatcctgttt ctgtaaattc attataccag ggacggtaaa ttgctttttg
     5581 atccttgata tttggcaaat ctatctgagg agattcaaaa taatcatacc aataccgcca
     5641 gccatcattg ggatatgtac cataatttgt tatcaatgct tttggatgtc tttcgagaat
     5701 tggccttgta tagcatattt ccaacagttt actgcgcaaa gaccttaatt tggactggaa
     5761 tacataaaaa tcctgaatat tgtctatatt tttacaacaa gtcatgcatt tttttgaatt
     5821 tacccatgct tcattccatt catgaggtcc gtctatttcc caatccgcag taacaatatc
     5881 tatatttacc cctgcagcct gatatgcttc cgaataagct ataatccggc tcctgataac
     5941 aggtatccgt tcctgaagtg tgaaaggaca tcccatccaa tgtcctgcaa atgagctatc
     6001 ataaaatgtt ctcccttcat tgtcaatatg tgctgtaaaa tcggtcttat cataaaaccc
     6061 gtataaaaga tcagttgcat caacagcaac ttttaatccc aggttctcct gaagcctggc
     6121 gatccggatc ccttcttcaa tacccctttc aaatgcttca gcctgtttcc agaatgtaat
     6181 aactccaatt ccacgttttg acaattcttt gattaccagt tcggtatctt tatcagagag
     6241 tctgctcaaa tcaccaattg aatactgata tattaattcc ctgtttcctc tttcataaga
     6301 taacggagaa agtttttcca acaggattct gatattatca ggaatttcct gcgaaagaat
     6361 tttataatca gatgaactta ttgttaaaag gaaaatgaag gctgcaagtg aacgaataaa
     6421 agctgttatt ttcatattgt atgaattagg agttaaaagg tacaaatttg tcaaagaaca
     6481 gaactttccg gcccgggcaa gcttgaaaat caatttttgt gagagttcat tttgtcagtt
     6541 tcaaaatttc acttgccagc atctcccggg cagcaaagat atcatttggg ttatcagttg
     6601 tttcccacca atttggcgcc acttttgata ctattttcaa tacagcttcc ctgcctgcca
     6661 gtttctcata aagattcaaa tattcataat cctccattga ttcacgcagg tttttcaaac
     6721 gtatactgga tatgggccca acaatgccac aaggcactcc gggataaata aaatatccac
     6781 ctccgttaaa atgcgttccg aagtggctaa aagccggatg ataccaggtg tccatcacgg
     6841 ttgtagtcaa agaccaataa aggatccctt taatgccgta ttgataattc atccatgtgg
     6901 gtaccctgtg tgatgttaat ggtgcatcga tatgccagta aggcgaatca taatttttaa
     6961 cctcgtcata atgtgggtga tacctcggcg ccctttgaga aagtgctgtg taagaccata
     7021 cttcatcacc ccggcttatt gcatcagtaa ctgattcacg atcgataaaa ccaaacaagg
     7081 gacaccagat atccaccgct gaatcaatat caggccagga aggatcatgg ctgtaaggtt
     7141 gttccacaac aagaatcttc agctgcgggg cacctttatg aacatcctcg gcaagttcca
     7201 gtactctttt atagcattct ggtgtatttg gctcatcata caaataaaca taggcgcgtt
     7261 tatcccatcc attttctttg agatacttat aaaaatcgct gtaatatcta actgcttttt
     7321 ctctgtcaga tgcattaagt gccttgtgcg gatcagtaaa tcctgctatc ggagcccgtg
     7381 gaatttcaaa atcactcaca ttaaagtctt ctataaattt tttcagttca ttatgtcttt
     7441 caggtatgat tttcagagaa ccatctgtgc tcatttcagg catcagataa tgagggaagg
     7501 gtggattcag acgatgttcc gtataaatcc tgcaatattc catttctatt ttcttatatt
     7561 catccgaatt aacctcgaca ccaaatcgtt cagagatcct tcccactctt ccaaaatggt
     7621 ttcgatgagt tgtttgatcg ggcagtgtga aatcccaaat cgttatggat acgggaacag
     7681 atatggtttt aactctgata ctgtcagcaa ccggccccca gggatcggga aaattatcca
     7741 gagtaacagt gaaagtacct tcatattcac cggcaacagt attttccgga acaaagacat
     7801 caacccatat aggctggttt tgacctttcc atacatcaaa gggcagggca tacatttcat
     7861 atcctctgct taccacagga ccgggccaat actctcttac ctgtgtacgt ggctcaattg
     7921 gtttgcctgt ctgtggattg ataaaaggta caagaggatc tgtatacaac ccggggggaa
     7981 gttgagcacg ggctgacgga cggctgattc ttatatattc agggcggtac agcgtgaaat
     8041 tttcttttga aaagattcca gccctgcctg aaagatctga tatttcggca ttgactaccc
     8101 tgatattttt caatatagcg ccgactacaa cctgaaatga ttcatattca tttttggcag
     8161 ctttaatttt aacttgcaat tcaccaaata attgatcatc ctgccctatt ctttccagac
     8221 tggaaattac agttagcttc acctcctctt tctcattgtt atttaataaa cacgaactga
     8281 ggatcatagt tatggcaatt gtaaaaactc tgatgactgg aaaatggttc tgtttcattt
     8341 taaatcctcc ttatttttta attaactcta aaaatacgaa caatctcctt tttgtatttg
     8401 ccggaggttg ccggaattta actgtatgtt ggtttggtgt tactgtatca gcttctcaat
     8461 actgaaaaaa acccactgat ccggatgcag taaaatgtcc ttatacattc ccatgggtca
     8521 atacatatta tttttcagga tgctgaatct taatgaagac tgtaaatgtt ttctgagcat
     8581 acgtctgatc tgaatcttat tctttcagga tgtatttcaa cggtactctt aaaaagcgtg
     8641 cccggtaggc ttcaacgtca tcaaatatat ccctgatgtt gagggaattc acgcaatagc
     8701 tcacaagaag ccggtcattt tctatgtact gtctgtgagc aagggcatta taggtaataa
     8761 tgtgttgatt gggatcttta tccggctcag gcgtgtggta aatgaatttt ttattatgaa
     8821 atcccgtgta cggcaaatct gaaatgtaag tgtagatatc attccctgct ctttcctgtg
     8881 taagcaggat gtacttattc cggtatctga aaatactgaa ctgttcggat acagaaatat
     8941 cgatatccat gggttctgat ttgccgatat ccttatccca gccttccccg gtgtaatatt
     9001 cccagtcgcg gtaaaaccga ctgcttcccg ccggaacacg ggcaacatga atctgtgcat
     9061 ggaaaggcag gttttttgta ccatatatat atatgtaatc ctcatcttcc agaacacagt
     9121 tcccgaaatc aatgagattt gcagggtaat caatatctgc cctccgggtt tccagatgcc
     9181 tgaagccggg caggctgaag gaaagcagat cgacatgggt aaccgcgaaa gtaaaaatat
     9241 tttctggttc actgtcaggg gaagtgctgc cattctgacc ggcgggatcc gttgttccgg
     9301 ctgtgtcggg aatatttacc aggcccagtg caaaaagata gagggtgtcc tttcttacaa
     9361 agccatgtcc gggccagtac cagcggtaag ttgaatcgcc cggttcattg accggttcca
     9421 tcagtgtcag tggattttcc cagcttcccc tgtaaaccgg ttcagcagaa gttctgtttt
     9481 tatctattac cacaaaggca tttctcagca ttgtggtgtt tggatccctg gtaccgttaa
     9541 ccactttccc gagaaaacag tctcccagca tgaacagcga ggaaccgtcg ggaagaagca
     9601 gtgagaacag tccgtctgct cccgtgatgc caccggaatc tgaatagaaa aagatacgga
     9661 aagcctcatc agtggtgatc ctgttcactg atccctcctg cccgcaggat gtcacggaaa
     9721 tatatatgac catccagaac agaggatata tcaacaagcg gtaacttctt gccttattca
     9781 ttttaaattt ccttttcata tttattcctc aacatatttt ctgatcgcct ctttccagac
     9841 ctggtatcct tttccgttaa gatgcaaacc gtcataactg tattccggat tgagtctgtt
     9901 gttttccaga cggaagagtg agaaaagatc aatatattca agcccgttaa gggacgcaat
     9961 ttccttcagg cggtcattga ccgcaataat atcgctgttg cggcgggtgt agtgaacggc
    10021 atcatccacc ggcagaacgc tctggatata gatttttgtc gcaggagtgg attccctgat
    10081 ccgctctatt attcttttga aattttctgt aatgtagtcc acggatctgc cttcggacag
    10141 gtcattggta ccgatcatga gaaaaatttt caggggccgg gattcagtaa cctcatccag
    10201 tctttccagg ataccgtcgg tgacatcacc gccaataccc cggtttttta tccgcgggtt
    10261 gccgaacatt tccgaccatt cagcctgatc agtaatgctg ttgccaagaa atattatttc
    10321 atcgtcagca tttggcaggg tctcaaaatg ttccttcctg tgatagtaat aggccttcat
    10381 ggctttgttc cagagctcga ggtcttcagc aaacctgtca tatttcatta tctccccgat
    10441 ctctgcttcg gtcattcccg agctcattga cggaggagca ttttccggcc gtatacccca
    10501 ggttttgtca ggtcgggatc ccatccgcag aaccaggttc ccccccgatg aaagatctgt
    10561 atgaggaaac catggccctg tcagttcctt cccgttcagg gcagccttcc ttacataaat
    10621 gtttccggat gagttattcc cggcctcaat gacaaatttc ccgcccgggt agtatttgcc
    10681 gtccagcctg atggtaatcc tgtcaaaaag aggacttccg atctcataac agggccgggc
    10741 agaggttccg ccgtcggtct gaaacaaacc gattgctccc agtgcataaa gtgctgcaga
    10801 ctgctcagga tagcataaaa cagagctcag gggtgaatga tgctcaccat atcccgaaag
    10861 aatctctctc agccacttct gtgcaagcca cggagctccg gcaaagttaa aaaggtatgc
    10921 tgcctgcagg ggagactgaa ccggaggaca gtccccggaa taaagcatct ttttcagcag
    10981 gtctgctatt gcttcctcct gaccggcatg tgccagatga cgtgaaatct ccttttcaag
    11041 cctgacggtg aattcttcct ttccgtacac agtgatgagt cctgctgcat catgcggaac
    11101 aaaattctgc atcacatctt cttccgggcc gctcacattt ttgtatttga aggctctccg
    11161 ggcgaatgtc atgtaatcgg acacatttcc cgaggcgcgt gccatctgtg acataaccca
    11221 gtcgtggtat gcatattcac ggataataca ggcaggcggt ccggccgggg taactgaatc
    11281 tgagagcagg tattcttcaa gatatttcct gccgctgtat ccgccgcctg catgtgcctg
    11341 gccgggactt gtcagatcat gctgtactgc cctgtacaga tcctgtatat cgtaatcatg
    11401 tattcccttc tgccgtgccg ccgccagtaa ggggatcaca tgggagctgg cggaaagtcc
    11461 cggatatcct ataccctctg agttatccgg gaaccagcct ccgtgcgaat gaagatgaag
    11521 cattgattta acgtaattat ccgccatatc cggccggcaa agggtcagaa gctgatttac
    11581 ggcaattgtg ccgggtccgg gaggcaaaca accata
//
LOCUS       DAOSPX010000068        11270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5688_length_11270_cov_4.106108, whole genome shotgun sequence.
ACCESSION   DAOSPX010000068 DAOSPX010000000
VERSION     DAOSPX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5688_length_11270_cov_4.106108"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..386)
                     /locus_tag="PLZ75_05055"
     tRNA            complement(313..386)
                     /locus_tag="PLZ75_05055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(350..352),aa:Pro,seq:cgg)
     gene            651..2777
                     /locus_tag="PLZ75_05060"
     CDS             651..2777
                     /locus_tag="PLZ75_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOS71800.1"
                     /translation="MKKLIYLILILLIKFFGMSHALSQGNHITDSLFALLKESKEKAA
                     ILNSLANEYLYISLPESMKLASDALGAARKEKNLSEEAKSLHNTASALIILGKYDSAV
                     IILEKSHTLFDRLKNTEGILGISNSMAVINYLQGRLNESYSQYEHNLVQSKKEKLPSI
                     QVSALMNIGRINWLRGQNDVALQYYNEALNIADSLRNEYMQGMIHLLAGISCQDMGYY
                     ELATGRMLRATELFEKMNYLLKLPYAYNYLGSVYFELQENEKALTSFRKALDGFTEIG
                     DEWGKAMTYRFMGSSFRRLRLLDSALYFFRTALVISNNLNDGSGELYSRRFLAEVLFE
                     QGQQDSARILLSENINKSLLIGNIPERVNNLYDLGLLYIKEGTLQKGIRFLQDALIIT
                     DSLNLFYENMLIYKQLAETYEKMGEPVKALAHYKRYKSLSDTIFTAEKRKNIDELQLR
                     YETVKKNSEISHLKLEQLMQAERLRNQRILGYTLAALLILLFVSSAILWNSYNHKKKA
                     NREKEILLKEIHHRVKNNLQTISSLLSLQSHNIADPGVKNAVKESQDRVKSMALIHQM
                     LYQQERTSMIDFAKYLNQLTDSIRSSYCGPGMNFSCTVKCDAVEIDIDTAIPLGLIAN
                     ELIVNACKYAFHPSGNGELLVSLSRDISNKLMFRVKDNGKGMPADFRIDKTDTLGLKL
                     VSLLVRQLKGDIRYNNHKGTEFIITF"
     gene            2866..3609
                     /locus_tag="PLZ75_05065"
     CDS             2866..3609
                     /locus_tag="PLZ75_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS71801.1"
                     /translation="MEASGDIRILIVEDELIIAEDIRTKLISLGYNVTGIAMTADEAE
                     RLLESDRPDLVMLDILIKGERDGIDLARIIRENYKIPFIFLTSHADRSTVEKARSVLP
                     DGYLLKPFTDKDLFAAIEVAIFRKSDEAEKKYEELPGKILQDCIFIKKDYFLIKVKFD
                     DLLWMRSEGNYLELYCTGDKKHLIRSPLKDFLNKLPADSFLQVHKSYAVNIKHIDAIE
                     YTTISVENHSIPVGRKFIDAIRQALDIDL"
     gene            3996..6509
                     /locus_tag="PLZ75_05070"
     CDS             3996..6509
                     /locus_tag="PLZ75_05070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOS71802.1"
                     /translation="MKKILILVILLVLTGKVFQSAGQVSLNKWKISDPVNVFLPAFSD
                     KSSVDGKKFDKAALFESVHSCTEEITSWRELETGNNSIIPGINGRDQLVQMTGFLRTD
                     RWTKTDITISTNALFELYLDGRKIKSQTNVSGKPVKTGITVDNGIHQLLLKLLTTKDS
                     LVFSAEAAASGKDSEAVFDWSINPQRKLTINDILEGETVSNASLSPSGKFLLINVSEV
                     LPGSGKTQSYTRIYDTELKKDLISLRNTAIRADWLPSTDRLCYQVTKENISDIYLYDI
                     QKGEETLIAAGLKSPGRITWSPDESYFVFSGTAEADKTGDLKRVFSNEDRIPNSRNRY
                     NLFLYNLKTKMVQQLTAGNLSASLHAIRPDGSAILFSVSRTDYSEIPFSKQNLYEMDV
                     RTFQLDTIWKDKLYGGYCQYSPDGKQLLVTGGPETFGTTGVKVSEGRIPNSSDTQLYL
                     YDPEKKESTSLTRDFDPAVERAYWSKDGMIYLTAVERDYVNLYRLDLKKHSFNKIDLP
                     VDVTGTVDYAKDKAIAVYSGSSISMPQKLYLLNLRTGKSTLLMDPKEKQMKDVVFGNS
                     EEWNFVNKNGTTIYGRVYYPVDYDPAKKYPLIVNFYGGTLPTDRSFGGRYPKNTWAAR
                     GYIVYVLQPSGATGFGQDFSALHVNGWGREAIDDIIEGTRKFLSAHPSADAGNIGCIG
                     ASYGGFTTMMLQTRTDIFKTAIAHAGISDITSYWGEGYWGYSYSASATRGSYPWNRRD
                     IYVDNSPLFNADRFSNSILLLHGTSDTNVPVGESLQFYAALKILGKNVEMVLVSGEDH
                     HILDYKKRIEWHNTIIAWFDKMLKDQPDQWNELYPDKNL"
     gene            complement(6587..8644)
                     /locus_tag="PLZ75_05075"
     CDS             complement(6587..8644)
                     /locus_tag="PLZ75_05075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOS71803.1"
                     /translation="MAESCTTKTGIKENPFFTEWKTPFEVPPFDRIADEHYLPAIDSG
                     IALARQQIEAIAASEEAPTFANTIARLDRSNELLNKVAAVFFSQTSANTNPDLEELQV
                     EIAPKLSAFGDEVLLNTALFERIRSVWENRASEQLDAEELFLLDNLYKNFVRNGALLG
                     SEDQEKLKSLNQELSVLTVRFSQNVLAETNNYKLVIDNEADLKGLPETVVTTASEMAK
                     AEGMEGKWIFTTQKPSMIPFLQYAENRDLRKKLYDAYLNRGNNGNEYDNNKIVSDIIR
                     LRAERAKLLGYKTHADFVLETRMAKTPGNVLSLLNDLLRRSLIVAKRERDEMQKIVVA
                     EGGGFKLEPHDWWYYAEKLRKAKYDLDENELRPYFSLDNVRDGAFAVAGRLYGITFSR
                     INNIPLPHPDAQAFEVKEADGRHVGVLYMDFFPRESKRQGAWCGGYRGHEVVDGKEIS
                     PVVTIVGNFTRPAGNAPALLSMDDVNTLFHEFGHGLQALFSENRYNETTVAWDIVELP
                     SQIMEHWATEPEVLAMYARHYETGEVIPRDLVDKIRNSSYFNTGFDNVEVMAASLLDM
                     AYHSLEAPAQVEPQQFEKDVMKRIGLIPEIEPRYRTTYFGHMIGGYDAGYYVYTWAAV
                     LDNDAFEAFREKNNIFDQAVAGSFRKNILETMGTRDAEQMYLDFRGREPVIEPMLRNR
                     GLN"
     gene            9130..10353
                     /locus_tag="PLZ75_05080"
     CDS             9130..10353
                     /locus_tag="PLZ75_05080"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOS71804.1"
                     /translation="MIFTLSLKYLDMASKNRILSIDIMRGLTLLLMLFVNDLNMHVAP
                     RWLGHMEASFDGMGLADWVFPGFLFIVGLAVPFALSKRFFRGDSNADISRHIITRSVS
                     LLIIGVLMLNSTRVNAELTGLSMNLWALLMYIAVFLLWNDYPNARERIYTIAGYKLTG
                     LAILIYLVFKFRSGEEINNGSLITGWWGILGLIGWGYLVTAFTYLLFRDSVWKTLMAV
                     LFFLVLNILSSLRLLTFLDSLQPYLGVLIGGNTPFIVLSGLLAGVILKKLKSEENSNI
                     IPIFIALGVFCLVSGFILRRWFIISKIWATPSWGMICNGISFLIYILIYWIVDIKGRK
                     KWAGFIMPAGQHSLTTYLAPSVLYHLIWMTNLPILIYKQSQSPAIVIAGSIIWAVAMA
                     GLTALLVRFNIRLKL"
     gene            complement(10427..>11270)
                     /locus_tag="PLZ75_05085"
     CDS             complement(10427..>11270)
                     /locus_tag="PLZ75_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOS71805.1"
                     /translation="ARKLKRPQEEIDLFAKRAMNYRNVFDPGRKLMRGRNEDGSFQSP
                     FSPYKWGDAFTEGNSWHYTWSVFQDIAGLVELMGGKDEFVLKLDSIFEVPPVFDYSYY
                     GIVIHEIREMQIMNMGNYAHGNQPIQHMIYLYNYAGQPWKTQMRVREVMEKLYNYTPD
                     GYCGDEDNGQTSAWYVLSALGFYPVTPGTDEYVFGSPLFDRVILTFENGKRFVVDAPG
                     NSKKNVYINSISLNGKSFRRNYITHYELQRGGKLTFSMSAEANRKRGTEESTFPYSMS
                     TERK"
BASE COUNT         3196 a         2573 c         2438 g         3063 t
ORIGIN      
        1 gcagcgacaa tccagccatt ttacgggcag caattaatcg gtgtgcaaaa atgttattca
       61 tagctttcct ggtttgttga caaatataca atatatatac caattgtcaa cctttttttt
      121 tataaattaa ttttaatatt atcgtattta tcaataaaac aagcgtctaa actatatcag
      181 tataagataa tacgagtata aattgaagac cctgtgaaaa aagtaaaata ttatttttct
      241 accacttttt ctactacaaa gaaaaatgcc gctgtaattt attgatttac aacggcattc
      301 tgttctaatc agtcggggtg agaagactcg aacttccgac ctccacgtcc cgaacgtggc
      361 gcgctaacca actgtgctac accccggttt tgcaaagaac catttttaca tggtcttcag
      421 tttccgccga caaaaataag tattttttta taagtaagga aattatcata atccgggaca
      481 cagagctaat aaattgtatt agtgctttaa tttccagaga agacacagta agctggtctg
      541 ttgcttatat gcacaaaaat tattaaattg tttacttgct taactgttta agtcagatta
      601 ttaaatactc tttcagaagt cgctttaaat tataaaacag gctataaaaa atgaagaaat
      661 taatatatct gattctgatt cttctgataa aattctttgg catgagccat gccctttcac
      721 agggcaatca tataaccgac agcctgtttg ccctgctgaa agaatcaaag gaaaaagctg
      781 caattctgaa ttcgctggca aatgaatacc tttacatatc actcccggaa tcaatgaagc
      841 tggcctcaga tgctttggga gcagcaagaa aagagaagaa tctttcggag gaggctaaat
      901 cattgcataa tacagcctct gccctgataa tcctggggaa atatgattct gcagtaatca
      961 tacttgagaa atctcatacc ctgtttgata gactaaaaaa tactgaaggt atacttggta
     1021 tcagtaactc tatggccgtg ataaactacc tgcaaggccg gctcaatgaa tcatactcac
     1081 aatatgaaca taatcttgtt cagtcaaaaa aggaaaaact gccctcaata caggtatcag
     1141 cactgatgaa tatcggacgt atcaactggc ttcggggaca aaatgatgtt gccctgcaat
     1201 actacaatga ggccctgaat attgctgatt cactcagaaa tgaatatatg cagggaatga
     1261 tacaccttct tgcagggata tcctgccagg atatgggcta ttatgagctg gctaccggac
     1321 gaatgctccg ggcaacagag ttgtttgaaa aaatgaacta cttgcttaaa cttccttatg
     1381 cctataatta cctgggatct gtttactttg agttgcagga aaatgaaaaa gcactgactt
     1441 ctttcagaaa agctcttgac ggcttcacag agattggaga tgaatggggt aaagcgatga
     1501 catacagatt tatgggcagt tcattccggc ggttacgctt actcgattct gccttgtatt
     1561 ttttcagaac cgccctggtt atttctaaca atctgaacga tggaagcggt gaactctatt
     1621 cccgcagatt cctggccgaa gttctttttg aacagggaca gcaggacagt gcccgtatat
     1681 tgttaagtga aaatatcaat aagtctttgc tgatagggaa catcccggaa agggtcaata
     1741 acctgtatga cctcggtttg ctttatatca aggaaggaac tctgcaaaaa gggataagat
     1801 ttttacagga tgcattaata attaccgatt cactcaacct cttttatgaa aacatgctga
     1861 tatacaagca gcttgccgaa acatatgaaa agatgggaga accagttaag gcccttgcac
     1921 attacaaaag atacaaatca ctcagtgaca ccattttcac tgctgaaaaa aggaaaaata
     1981 ttgacgaact tcaactcagg tatgagacag ttaaaaagaa cagtgaaatc agtcatctga
     2041 aactggaaca acttatgcag gctgaacgat tgcgaaatca acgcattctg ggttataccc
     2101 tggcggcatt actgatattg ctgtttgtct cgtcagcaat tctctggaat agttacaacc
     2161 ataagaaaaa agccaacagg gaaaaggaaa tccttttgaa agagatccat cacagggtaa
     2221 agaataattt acagacaatc tccagtcttc tgagcctgca gtctcataac atagctgatc
     2281 cgggagtaaa aaacgctgtg aaagagagcc aggaccgtgt caaatcaatg gctctgatac
     2341 atcagatgct ctatcagcag gagagaacct caatgattga ttttgcaaag tacctgaatc
     2401 agcttactga cagtatcaga tcaagttatt gcgggcccgg aatgaatttt agctgcactg
     2461 tgaaatgcga tgctgttgag attgatatag atactgccat accactggga cttattgcaa
     2521 atgaactaat tgtcaatgcc tgtaaatatg ctttccatcc ctcaggaaat ggagaactgc
     2581 ttgtttctct ttcaagggac atcagcaata aactgatgtt cagagtcaag gacaatggca
     2641 aaggtatgcc tgcggatttc agaattgaca agactgacac actgggtctg aagcttgttt
     2701 ccttgctcgt gaggcagctg aaaggggata tcaggtataa taaccataag ggtactgagt
     2761 tcattatcac cttctgaatt tattgcgata ttgtttttat agtgttaatt tcgcattgga
     2821 gaaaacacta cagtaaaaga ctttcctgcc ataaagcagc ttttcatgga agcatcgggc
     2881 gacataagga tattgattgt ggaagatgag ctgataatag ccgaggacat ccgcaccaaa
     2941 ctcatcagtc tcggatacaa tgtaacagga atagcaatga ccgctgatga ggccgaacgg
     3001 ttacttgaat ccgacaggcc cgacctcgtt atgcttgata tactgataaa gggcgaaaga
     3061 gacggtattg accttgcccg cataatcagg gaaaattata aaataccctt tattttcctg
     3121 acatcgcatg ccgacagaag tactgttgag aaagccagaa gcgttcttcc cgacgggtat
     3181 ttgcttaaac cctttaccga taaagacctg tttgccgcaa ttgaagtggc aatcttcaga
     3241 aagagtgacg aagcggaaaa gaaatatgaa gaactgccgg gcaaaatact tcaggattgt
     3301 attttcatta aaaaggatta tttccttata aaggtgaaat ttgatgatct tctgtggatg
     3361 agatcggaag ggaattatct tgaactgtac tgcacgggag acaaaaaaca tcttatcagg
     3421 tctccgctga aggacttcct gaataagctt cctgcagatt cattccttca ggttcacaaa
     3481 tcctatgccg taaacataaa acacatagac gctatagaat acaccactat atcggtggaa
     3541 aaccacagta ttcccgtggg acggaaattc attgacgcga tcagacaggc tcttgacatc
     3601 gatctgtaac agctttcaga atcgcttcct gtttgcgccc tctcccgtgt gtcagatgtg
     3661 gccggccgtc agtcaggcgg taacgggata catcagagga aaaagcagtg gaagtccgcg
     3721 cttccgttcc tggcccttca accacagcac agtagtcaaa agcgccatca aaaggagaag
     3781 ggcgctttta tgagttgcct aaagttcaga cagggactgt gtattctatt ttatcacatg
     3841 aagaatatgt cttctctgca ttttattata actttcggcc tgaagttgaa atgatctcag
     3901 tcacaaacac gaaatgatat agatcagagt catgcatgac ttccccgctt tcgcagggca
     3961 gacatttata gcataaaaat caaaattcta ttatgatgaa aaaaatttta atcctggtta
     4021 ttctactggt tcttacagga aaagtttttc agtcagcagg ccaggtttcc ctgaacaaat
     4081 ggaaaatatc agatccggta aatgtgttcc tgccggcctt ttccgacaaa tcatccgttg
     4141 atggcaagaa atttgacaag gccgctcttt ttgaatcggt tcattcctgc actgaagaaa
     4201 tcacatcctg gcgggaactg gaaacgggaa ataactctat catacccggg ataaatggca
     4261 gggatcagct ggtacagatg acaggctttc tcaggaccga ccgctggaca aagactgata
     4321 ttacaatctc caccaatgcc ctgtttgagc tgtatcttga cggcaggaaa ataaaatctc
     4381 agacaaatgt atcaggcaag cctgtaaaga ccgggattac cgttgacaac ggaattcacc
     4441 agctgctgct gaaactgctg acaacgaaag attctcttgt gttttcagct gaagccgctg
     4501 cttcaggcaa agactcagag gcagtctttg actggagtat caatccgcaa cgcaaactaa
     4561 cgataaatga tatacttgaa ggtgagaccg tctcaaatgc cagcctctcc ccttccggaa
     4621 aattcctgct tatcaatgta tccgaagtac tgcccggcag cggcaaaaca caaagttata
     4681 cccggatata tgatacagaa ctgaaaaagg accttatatc cctgcggaat acagccatcc
     4741 gcgccgactg gcttccctcc acagacagac tgtgctacca ggttacaaag gaaaacattt
     4801 ccgatatcta tctgtacgat atacagaagg gtgaggaaac actgattgca gccggactga
     4861 aatctccggg aaggataaca tggtcgcccg atgagagtta tttcgtcttc tccggtactg
     4921 ccgaagcaga caaaaccgga gatctgaaac gtgttttcag caatgaggac cgtataccaa
     4981 acagccggaa ccgctataat ctgtttcttt acaatctgaa gaccaaaatg gtacagcaac
     5041 ttaccgcagg aaacctgtct gcatcattac atgcaatcag gccagatggt tctgcaattc
     5101 tcttctccgt atcgaggaca gactactctg aaataccttt ctcaaaacag aatctgtatg
     5161 aaatggatgt caggaccttt cagcttgata ccatctggaa agataagctt tacggaggtt
     5221 actgccagta ttcacctgac gggaaacagc tgctcgtaac gggcggaccg gaaacattcg
     5281 gcactacggg cgtgaaggtg agtgagggaa gaataccaaa cagcagtgat acccagcttt
     5341 atctctatga tccggagaag aaggagagca caagtcttac ccgtgacttc gatccggctg
     5401 tggaacgggc ttactggtca aaggatggaa tgatttacct gacagcagtt gaacgtgact
     5461 atgtaaacct gtatcgcctt gatctgaaaa agcatagctt caataagatt gatcttccgg
     5521 ttgatgttac cggaacggtt gactacgcca aagacaaggc aattgccgtc tattccggca
     5581 gcagtatcag catgccgcag aaactgtatc tgcttaatct caggacagga aaatcaaccc
     5641 tgctgatgga tccgaaagaa aagcagatga aggatgtggt gtttggcaat tccgaggagt
     5701 ggaattttgt aaataaaaac ggaacaacca tttatggacg tgtttattat ccggttgatt
     5761 atgaccctgc aaaaaaatac ccgctcattg tgaatttcta tggcggcaca ctgcccacgg
     5821 acaggtcttt tggcggacgt tatcctaaaa acacctgggc tgcaagaggc tatattgtct
     5881 atgtacttca gcccagcggg gcaaccggtt ttgggcagga tttctctgcc cttcatgtca
     5941 atggctgggg ccgtgaagcc atcgacgaca tcattgaagg caccagaaaa ttcctcagcg
     6001 cacatccctc tgccgatgcc gggaatatcg ggtgcatagg tgcttcttac ggaggcttca
     6061 ccaccatgat gcttcagacc cgtaccgaca tttttaaaac ggcaattgcc cacgcaggta
     6121 taagcgatat cacaagttac tggggcgagg gttactgggg atattcatac agtgccagcg
     6181 caacaagggg aagctacccc tggaaccgcc gcgacatcta cgtggataac agtcctcttt
     6241 tcaatgccga cagattcagt aactccatcc ttcttctcca cggcacttcc gataccaatg
     6301 tgcccgttgg cgaaagtctt cagttctatg cagcactgaa aatcctgggg aaaaacgtgg
     6361 agatggtcct cgtcagtggc gaagaccatc atatactgga ttataaaaag agaatcgaat
     6421 ggcataatac cataatcgcc tggttcgata aaatgcttaa ggaccagccc gatcagtgga
     6481 atgaactgta tcccgacaag aatctctgaa caactggcgg tctgcctgca gtactgtcag
     6541 aatcagtatg ttcttcaaaa aatcctgaca gtacagcaga ccttcttcag ttcagacccc
     6601 tgttcctgag catgggttct ataacgggtt cacggccccg gaaatcgaga tacatctgtt
     6661 cagcatccct tgttcccatt gtctcaagga tattcttcct gaatgaaccg gcaacagcct
     6721 ggtcgaaaat attattcttc tccctgaatg cctcaaaggc atcattgtca aggacggcag
     6781 cccaggtata tacatagtat cctgcatcat atccgccgat catgtggcca aagtaggttg
     6841 tacggtatct tggttcaatc tccggaatca gtccgatcct tttcatcaca tccttttcaa
     6901 actgctgtgg ttcaacctgt gcaggtgcct caagtgagtg atatgccata tcaagcaacg
     6961 atgcagccat cacctcaaca ttatcgaatc ctgtattgaa ataactgctg ttccttatct
     7021 tgtcgaccag atcccgggga attacctctc cggtttcata atgccttgca tacatggcca
     7081 gcacttcagg ctccgttgcc cagtgctcca tgatctgtga gggcagttca actatatccc
     7141 atgcaacggt ggtctcgttg tacctgttct ccgaaaagag ggcctgcaag ccgtgaccaa
     7201 actcatgaaa cagggtgttt acatcatcca tgctgagaag ggcaggtgca tttcctgccg
     7261 gacgggtgaa attgccgaca atggttacaa ccggacttat ctctttgcca tcaacaactt
     7321 catgaccgcg atagcctccg caccatgctc cctgcctctt gctttcgcgc gggaagaaat
     7381 ccatataaag tactcccaca tgcctgccgt cggcttcttt tacctcaaag gcctgtgcat
     7441 cgggatgagg aaggggaata ttgtttatac gggagaaagt gatgccatac agtctgccgg
     7501 caacggcaaa ggcgccgtca cggacattgt caagactgaa atagggcctg agttcattct
     7561 cgtccagatc atatttggcc ttacgcagct tttcggcata ataccaccag tcatgaggtt
     7621 caagcttaaa tccgccgccc tcggcaacaa ctatcttctg catctcgtca cgctcacgct
     7681 ttgccacaat cagcgatctc ctgagcaggt cgttcagcag gctgagtaca ttccccggag
     7741 tttttgccat gcgggtctca agaacaaaat cagcatgcgt cttatatccg agcagttttg
     7801 ccctttcagc tctcagcctt attatatcac tgacaatctt gttattgtca tattcattcc
     7861 cattgttgcc acggttcaga taggcatcat aaagcttttt acgaagatcg cggttttctg
     7921 catactgaag aaaggggatc atgctgggct tctgggtggt aaagatccat ttgccttcca
     7981 tgccttcagc ctttgccatc tctgaggcag tggtgacaac agtttcagga aggcctttca
     8041 gatcagcctc attgtcaata acaagcttgt aattatttgt ctcggcaagt acattctgac
     8101 tgaaacggac agtcagaacc gaaagctcct ggttcaggct ttttagtttt tcctgatcct
     8161 cagaaccaag cagggcaccg tttctcacaa aatttttata aagattgtcg agcaggaaaa
     8221 gctcttcggc atcaagctgt tctgaagccc tgttttccca aacgctcctg attctctcaa
     8281 aaagagccgt gttaagcaat acctcatcac cgaatgccga cagcttcggg gctatctcta
     8341 cctgcagctc ctcaaggtca gggttggtat tggcacttgt ctgcgaaaaa aacacggctg
     8401 ccaccttgtt cagcaattca ttggaacggt caaggcgggc tatggtgttg gcaaatgtcg
     8461 gtgcctcttc actggcagca atcgcttcaa tctgctgccg ggccaatgca atgcccgaat
     8521 caatggcagg caggtaatgc tcatcagcaa tccggtcaaa gggcggtact tcaaagggtg
     8581 tcttccattc cgtaaaaaag ggattctcct taatacctgt ttttgtagta catgattcag
     8641 ccataatcag gactaatatc acaaaaaaac atactttttt catttgtcta taatttaatt
     8701 taaatactcc gatacctgtc ccgcgtaagc gggtgcttgt cccggttcat cgggacctgt
     8761 cccgcgtaag cgggtgcttg ttcccgattt atcgggtacc tgttccgcga aagcgggtac
     8821 ctgtcccgat ttatcgggag tttgttccgc agaagcagcg gaatttccat gaatccgaag
     8881 tcatacccgt gtgcaggttc tttacatggt cgtttcattt tgaattttac aatgggttta
     8941 ttcgaagcgt aaagttaaac tttttatctg attctgatat acacagcatc cctctctccg
     9001 gggctgtgag caggatggca aaaaaagcct tgcccctgag accggtttca gatttcccgc
     9061 agaaaaaatg aactgatcag ggtacgatga tgaggtaaat aagggaaagg aaggaattgg
     9121 aattttttta tgatatttac actttcctta aaatacctcg acatggcatc caaaaaccgt
     9181 atcctgtcaa ttgatataat gcgcgggctg acattgctgc tcatgctttt tgtcaatgat
     9241 ctcaacatgc atgtggcacc ccgctggctc gggcacatgg aagcaagctt cgacggcatg
     9301 ggactggccg actgggtttt tcccggattc ctgtttattg tgggactggc agttcctttc
     9361 gctctttcaa aaagattttt caggggtgac agtaatgcag atatcagcag acatatcatc
     9421 acaagatcgg tcagcctgtt aataataggt gtgcttatgc tgaactccac cagggtcaat
     9481 gctgaactta ccggactaag catgaacctc tgggccctgc tgatgtatat agctgtcttc
     9541 cttttatgga acgattaccc caatgccaga gaaaggatct atacaatagc aggatataag
     9601 cttactggac tggctatact tatttacctg gtattcaaat tccgttccgg ggaagaaata
     9661 aacaatggtt cccttataac cggatggtgg gggatactgg gcctcatcgg ctgggggtac
     9721 cttgtcacgg cattcacata tctcctcttc cgcgattcgg tatggaaaac actgatggca
     9781 gtgctcttct ttcttgtgct caatatttta tcttctctca gattgctgac ttttcttgac
     9841 tcgcttcagc cataccttgg tgttcttatc ggcggaaaca cacctttcat tgtattgtcg
     9901 ggactgctgg ccggagtgat actgaaaaag ctcaaatcag aggaaaacag caatatcatc
     9961 ccgatattca tagctctcgg agtgttttgc ctggtctccg gattcattct ccgccgctgg
    10021 ttcatcatct cgaagatatg ggccactcca agctggggga tgatatgcaa cggaataagc
    10081 tttcttatct atatccttat atactggata gttgacataa aaggaaggaa aaaatgggcg
    10141 ggctttatca tgcctgccgg acaacattcc cttaccacat accttgcacc ttcggtattg
    10201 tatcatctga tctggatgac aaatcttccg atactcattt acaagcaatc ccagtcgccg
    10261 gcgattgtga ttgccggatc aataatctgg gcagtggcaa tggcaggact gaccgcactt
    10321 cttgtgcgct tcaacatcag actgaaactg tagaaagcct tccgaagact gccggaagct
    10381 tatcataaag ttttcgatcc tgtcttcttt agttactttc tgtattctat tttctttcag
    10441 tactcattga atagggaaat gtactttctt ctgttcccct tttcctgttt gcttcagccg
    10501 acatgctgaa tgtgagcttt cctcctctct gaagctcata atgagttata taattccgtc
    10561 tgaatgattt tccgttaaga ctgatactgt ttatatacac attctttttg ctgttacccg
    10621 gagcgtccac gacaaacctt ttgccgtttt caaaggtaag gataaccctg tcaaaaaggg
    10681 gtgatccgaa aacatattcg tcggttccgg gtgtgacagg ataaaagccc agggctgaaa
    10741 ggacgtacca tgccgatgtc tgtccgttat cctcatcacc gcaataacca tcgggtgtat
    10801 aattgtaaag cttttccatt acctccctta cgcgcatctg tgttttccag ggctgaccgg
    10861 catagttgta aaggtaaatc atgtgctgta tcggctgatt gccatgtgca tagttaccca
    10921 tgttcatgat ctgcatttcc cgtatttcat gaatcactat gccataatag gaatagtcaa
    10981 acacgggagg gacctcaaaa attgagtcaa gcttaagcac aaattcatcc ttgcctccca
    11041 tcagttcaac aagtccggca atatcctgaa aaacactcca tgtatagtgc cagctgttgc
    11101 cttccgtaaa ggcatcaccc catttatacg ggctgaaggg cgactggaag gaaccgtctt
    11161 cattcctgcc tctcatcagc ttccgtcccg gatcaaacac attcctgtag ttcattgccc
    11221 gcttcgcaaa gaggtcaatc tcctcctgcg gccttttaag cttcctggcc
//
LOCUS       DAOSPX010000069        10924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5887_length_10924_cov_4.074064, whole genome shotgun sequence.
ACCESSION   DAOSPX010000069 DAOSPX010000000
VERSION     DAOSPX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5887_length_10924_cov_4.074064"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PLZ75_05090"
     CDS             complement(<1..864)
                     /locus_tag="PLZ75_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOS71806.1"
                     /translation="MERQNDKKSFGETLRLLKPKIIDLYIIRKFLGTFLFSLALILAI
                     AVVFDLSEKLDNFMEHEAPWQAVIFDYYFNFIPFFATLFAPLFVFIAVIFFTSRMAVN
                     TEIIAILNSGMSFHRMMWPYFLSSFVIAGVIFCLANFVIPHSNLVRLDFEDKYYRPRG
                     RRINIENVHRQVSRNVYVYIESFSPIYLRGRNFTIEKFDEEGNLLSKISAPTVTWDSV
                     TSKWSIMNYVYREINGHEETVTKGSVMDTTLNIKPEDFSRDPSFVSTMTYRELDEYIE
                     LLELQGSEEIKL"
     gene            complement(822..1952)
                     /gene="tgt"
                     /locus_tag="PLZ75_05095"
     CDS             complement(822..1952)
                     /gene="tgt"
                     /locus_tag="PLZ75_05095"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOS71807.1"
                     /translation="MMFELRSRDKDTKARTGILKTGHGIIHTPVFMPVGTAGTVKGIL
                     QRDLEEDLDARIILGNTYHLYLRPGMEVLRESGGLHSFISWNRPILTDSGGYQVFSLS
                     GNRKLTDEGVIFKSHIDGSRHIFTPENVIDIQRVIGGDIIMALDECAPFPCDHGYAAK
                     SVELTHNWLGRAVERFRNSDPLWNHRQYLFPIVQGGVFDDLRRMSAERIAETGMAGNA
                     IGGLSVGEPADEMYRIIEIVNEILPEDKPRYLMGVGTPANLLEAIERGTDMFDCVMPT
                     RNGRNGMLFTSQGIINIKNRRWISDFTPIDPEGASEVSLRYSKAYLRHLVVSGEMLGG
                     QIASLHNLAFYLKLMSDARRKIEEGEFKKWKDKMIKKVSVRL"
     gene            2083..3216
                     /locus_tag="PLZ75_05100"
     CDS             2083..3216
                     /locus_tag="PLZ75_05100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS71808.1"
                     /translation="MIYHDNLFLSVVFIIFAFSAAVQLFHYLCFYLAVSLYRHPAEEE
                     TEKKPVSVIICARNEAENLRKFLPSILKQDYPEYEVIVVNDCSEDNTYDVLGSFLPDH
                     PHLKISTINKDPRFTHNKKFAQFIGIKAASNDILLFTDSDCEAVSPRWIEMMASRFRD
                     DIQIVLGYGGYMKGKGLLNKYIRYDTFMIALQYLGMAVRKIPYMGVGRNLAYRRSLFF
                     EKKGFEGHTHIISGDDDLFVNANATGRNTSVEIRPESHTRSVPCPTFECWVKQKKRHF
                     TSAPLYKTWHKLIIGSELLSRILFYIMFIILIIPLNLWQVIAAVFGLRLIVQIIVMIK
                     AQKILNEKGIAGFSLFFDIFSPVINSVIFLSNTITRPGKNKWK"
     gene            3207..3803
                     /locus_tag="PLZ75_05105"
     CDS             3207..3803
                     /locus_tag="PLZ75_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS71809.1"
                     /translation="MEINQELSEKAKADLILVEDAKKGSEKAFAGLMNRYRDSIYFML
                     LKMVNNPVDAEDLTIEAFGKAFRSIDSYTPRFAFSTWLFMIATNNCIDFIRKKQASPI
                     PVNQDKDIMDTMTVNIQSDLPDPEEALINNQKIATLRKIVAQLKSPYREIIELRYYRE
                     YSYEEIATELKIPIGTVKAQLYRAKTLLYNIFVKTGAR"
     gene            3803..4449
                     /gene="rsmG"
                     /locus_tag="PLZ75_05110"
                     /pseudo
     CDS             3803..4449
                     /gene="rsmG"
                     /locus_tag="PLZ75_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
     gene            4648..4806
                     /gene="rpmH"
                     /locus_tag="PLZ75_05115"
     CDS             4648..4806
                     /gene="rpmH"
                     /locus_tag="PLZ75_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOS71810.1"
                     /translation="MKRTYQPSRRKRANKHGFRERMSTRSGRRVLSARRAKGRKKLTV
                     SSELSGKA"
     gene            4873..5973
                     /gene="mqnE"
                     /locus_tag="PLZ75_05120"
     CDS             4873..5973
                     /gene="mqnE"
                     /locus_tag="PLZ75_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOS71811.1"
                     /translation="MTRILDHIIDTDLRETALKVASGKRITPDEGILLYERGDLPVLG
                     LLSSMVRKRINGNYAFYNRNFHIEPTNICIYNCRFCSYRKPAGDPESWDYSHDEIMNM
                     VRAFDGKNVTEVHITGGVHPAHDVHYYCSLVAKIKAHRPELHVKAFSAVELDHMIRLA
                     GLTTEKGLKLLKESGLDSIPGGGAEIFDEKIRKQISNEKASSEKWLNIHETAHRLGIS
                     SNATMLYGHLETWPHRIDHMNRLRKLQDKTGGFNAFIPLKYRMANNRMSQLGEVSIIE
                     DLRNYAVARIFLDNIPHIKAYWPMTGKIVAQLSLFFGADDLDGTIDDTTRIYSMAGAE
                     EQNPSMTAGEISSLIAGAGYEPVERDSLYRRV"
     gene            6029..6880
                     /locus_tag="PLZ75_05125"
     CDS             6029..6880
                     /locus_tag="PLZ75_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOS71812.1"
                     /translation="MSLKKFILSKLFLKQLGIAFLITIGTIFILMISLNIYTRHGQAV
                     PVPDFTGLNMEETKALAKKSKMKYQVVDSVYTSHVMRGCVAEQNPRPGFRVKKRRTIS
                     LTINAFRPEMVAVPDLLDLPLRQAISLIESTGLVMGTLRYKPDLSVNVVLSQLFNGKE
                     ITPGDSIQKGSVIDLVLGKGLSNQRTAVPNLIGLPLEDAAGRILGASLNLGTFIFDRT
                     VRTGEDSLRAFVYKQNPEYREDATLQLGSSIYLWMTTDSLKLPADSAFVRADTTMTTA
                     VLAGKVH"
     gene            6886..8730
                     /locus_tag="PLZ75_05130"
     CDS             6886..8730
                     /locus_tag="PLZ75_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS71813.1"
                     /translation="MIRRNLLNIAILLSFTGNLTGQEIVIGLTSNPVLKNTPSRSAKS
                     FSAEQLTLPFFDDFSGRDIYPDPAKWSDDNVFINNTYSRDQITTGIATFDALDRTGRL
                     YGEASPVTFRADVLTSQPIDLAYTAADNIRLSFFYQPGGIGDMPEKNDTLTLHFFAPD
                     ENKWYSVWRAYGDSSGIFRLAMLKIDDPRFLKTGFRFRFINYASLSRSKTDLSMTGNC
                     DHWNIDYVLLDKNRSSRDTIFHDVAFRKPFRSLLKNHEAMPPAHFNKIYLQEMGDNIP
                     ISYRNNDTIVRNVTRNFRIWDVYKNEEAISFSAGAANVGPLENVDYNASLFYTFNTIG
                     RDSALFKINAWLKTDDFDPKTNDTVTYFQVFRNYFAYDDGSAEAGYGINGLGSRNAMV
                     AVRFRAYTRDTLRAISICFNDSYMRANRRSFSLMVWNDNNGLPGDVIYTRQETMVEQA
                     GDINGFHTYFLPSGVAVEDNFYVGWKQQSETFLNAGFDLNTPHRGNLLYWINGSWFTS
                     QHSGTLMIRPVVGPPVRTSVHDVYHNPETPLRFHPNPARDYISFDRKDLTETDQYDVS
                     VFDLNGRELLKVKMTDKTDISSLKPGAYIIVLGRNGKAVGYSRLIKIK"
     gene            8739..9767
                     /locus_tag="PLZ75_05135"
     CDS             8739..9767
                     /locus_tag="PLZ75_05135"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOS71814.1"
                     /translation="MSEEEIAEKQELFEHYRFNVDPGQSLLRIDKYLFSRIENTSRTR
                     IRNAADAGNILVNDIPVRANYRVKPDDIVRIVLPDPPREIELIPEDIPIDIIYEDDDV
                     IVVNKAAGMVVHPAYGNYSGTLVNALMWHFRDLPLFATGELRPGLVHRIDKNTSGLLV
                     VAKNEISLNRLSRQFYEKTADRIYTAIAWGVPAPAEGTVTGHIGRNIRDRKIMQVFPD
                     GSRGKPAITHYKVLENLGYISLIECRLETGRTHQIRVHFSYIGHPLFNDDEYGGDRIL
                     KGTTFQKYRQFVRNCFSLLPRQALHARFLSFSHPVTGKRLSFDTPMPDDMQQVITRWR
                     EYISGRDA"
     gene            9801..10772
                     /locus_tag="PLZ75_05140"
     CDS             9801..10772
                     /locus_tag="PLZ75_05140"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOS71815.1"
                     /translation="MKTIAVVAGGDSSEYQISVKSGKSVAGIISSLYRVYLIIIRGSN
                     WYWEDPKGRYHTVDKNNFTLKADDLTIRFDAVFIAIHGKPGENGMLQGYFDMLQIPYT
                     GSSSFCSALTFNKHACKLFLKEYNIGMAESIFLRKNEKPDNAVIVNKLGLPCFVKPND
                     SGSSFGVSKVRQAGELADAISEAFRESDEILIESFMQGREVACGLVMTKSRKIILPLT
                     EIISKNEFFDFEAKYTPGKSDEITPADLPEKLTDEIRKLSSRIADLLGCSGIVRVDFI
                     IVGNKPLFLEINTVPGMTEESIIPKQAAAAGIPLKDLYSMVIEDLFV"
BASE COUNT         3164 a         2634 c         2445 g         2681 t
ORIGIN      
        1 cagcttgatc tcctccgaac cctgaagctc cagcaattca atatattcat caagttccct
       61 gtaggtcatc gtactgacaa aactgggatc acgggaaaaa tcctccggtt ttatattcag
      121 ggtggtatcc atcaccgatc cctttgtcac tgtttcttca tgaccgttta tttcacggta
      181 aacataattc attatcgacc acttgctcgt aacggaatcc catgtcacgg tcggagccga
      241 tatctttgaa agcagattgc cctcctcgtc aaatttctca atggtgaaat tacgtcccct
      301 gagatatatg ggactgaaag actctatata gacgtaaaca ttcctgctga cctgcctgtg
      361 gacattttca atgttgatcc tcctccccct gggacggtaa tatttgtctt caaaatcgag
      421 ccttacaaga ttggaatggg gtatcacgaa atttgccagg cagaaaatga cacctgcaat
      481 aacaaaggat gacagaaagt agggccacat catcctgtga aaactcattc cgctgttcag
      541 gatggcaatt atctcagtgt tgactgccat tcgggaagta aagaatatta ctgcaataaa
      601 cacgaacagg ggtgcaaaca gcgtggcaaa gaagggaata aaattgaaat agtaatcaaa
      661 tatcactgcc tgccacggag cttcatgttc cataaaattg tccagtttct ccgacaggtc
      721 gaaaactacc gcaatggcca ggatgagtgc cagactgaaa agaaatgtcc caaggaattt
      781 ccttataatg tagagatcta ttattttggg tttcagcaat cttaaagtct caccgaaact
      841 ttttttatca ttttgtcttt ccatttcttg aattcccctt cttcaatttt cctgcgggca
      901 tcgctcatca gtttcaaata gaatgccagg ttatgcagac ttgctatttg tccgccgagc
      961 atttcacccg aaacgacaag atgcctcaga tatgcctttg aatatctaag actcacttct
     1021 gaagctcctt caggatctat cggagtgaaa tcactgatcc agcgcctgtt ctttatattg
     1081 attattccct gtgatgtgaa aagcatgcca ttgcggccgt tcctggtagg catcacgcag
     1141 tcaaacatat ccgtccccct ttctattgcc tcaagcagat tggcaggcgt ccctactccc
     1201 atcaggtacc tgggcttgtc ttccggcaga atctcattca ctatctcgat tatcctgtac
     1261 atttcatctg ccggttcccc tacggagagg cctccgatcg cattacccgc catacctgtt
     1321 tccgcgatcc tttccgcaga cattcttctc agatcatcaa agacccctcc ctgtacaatc
     1381 gggaagagat actgcctgtg attccacagg ggatcactgt ttctgaatct ttcaacagcc
     1441 ctgccgagcc agttgtgtgt cagctcaacc gatttagcgg catacccgtg atcacaggga
     1501 aaaggcgcgc actcatcaag cgccatgata atgtcgcctc ctataaccct ctgaatatct
     1561 ataacgtttt ccggggtaaa aatatgcctc gatccgtcaa tatgggattt gaaaatcacc
     1621 ccttcatccg tcagcttccg gttccccgac agggaaaata cctggtaacc cccgctgtca
     1681 gtaagtatgg gcctgttcca tgaaataaaa ctgtgaagac ctcccgactc cctgagcact
     1741 tccatgcccg ggcgcagata caggtggtag gtgttgccaa gaattatccg cgcatcaagg
     1801 tcttcttcaa gatccctctg aagaattccc ttaacggtcc ccgcggttcc gacaggcatg
     1861 aaaacggggg tgtggattat tccgtgaccg gttttcagta ttccggtcct ggcctttgta
     1921 tccttgtccc tcgatcggag ctcaaacata attctgactt tggtagcaca aatgtaattc
     1981 atccgcaccg cctcaggaaa ttatgtgacg ctttttgttg aaaactattc ttcacaatta
     2041 aaatatatct tgctaatatt gtgtccggat tatgaaagtt caatgatata tcacgacaac
     2101 ctctttcttt ccgttgtatt catcattttt gcattctctg cagcggtgca gctattccat
     2161 tacctctgct tctatcttgc agtatccctt tacaggcatc cggcagaaga agaaactgaa
     2221 aagaagcctg tttccgttat catttgtgca agaaacgaag ctgaaaacct ccggaaattc
     2281 ctgccatcca ttctgaaaca ggattatcct gaatatgagg taattgttgt caacgattgc
     2341 tcggaagaca atacctatga tgtcctgggc agctttcttc ctgatcatcc ccacctcaaa
     2401 atatcaacaa taaacaagga tccccgcttt actcacaaca agaaattcgc acagttcata
     2461 ggcatcaagg ctgcctcaaa cgacatcctg cttttcaccg actcagactg tgaagctgtt
     2521 tccccgcgct ggattgagat gatggcatcc cgtttcaggg acgatattca gatagttctc
     2581 ggctatggcg gttatatgaa gggaaaggga ctgctcaaca aatatatccg ctacgacacc
     2641 tttatgatag cattgcagta tctcggaatg gctgtcagaa agatacccta catgggcgta
     2701 ggccgtaacc tggcataccg gcgttcatta ttcttcgaga aaaagggttt cgaaggccat
     2761 acccacatca tttcaggtga tgatgacctg tttgtgaatg caaatgccac cggaagaaac
     2821 acttccgtgg aaatcaggcc cgaaagccat acccggtcgg ttccctgccc cacctttgaa
     2881 tgctgggtta aacagaaaaa aagacatttt acctcagcac ccctttacaa aacatggcat
     2941 aaactgatta ttggaagtga gcttctttcc aggatattat tttatatcat gttcatcata
     3001 ctgatcatac ccctgaacct ctggcaggtc attgcggcag tattcggact aaggctgata
     3061 gtacagataa ttgtcatgat aaaggcacaa aagatactaa atgagaaagg tattgcggga
     3121 ttttcattat tttttgatat cttttcaccc gtgattaaca gtgttatttt tttaagcaat
     3181 acaattacca gaccaggtaa aaacaaatgg aaataaacca ggagctttca gaaaaagcga
     3241 aagccgatct tattcttgtt gaagatgcca aaaaaggcag cgagaaggct tttgcaggcc
     3301 tcatgaaccg ttacagggat tctatttact ttatgcttct caaaatggtc aacaatcctg
     3361 ttgatgcgga agacctgacc attgaagcct tcggaaaggc attcagaagt atcgacagct
     3421 acaccccgcg ctttgcattc agcacatggc ttttcatgat agccacaaac aactgcattg
     3481 acttcatcag aaagaaacag gcttccccta ttccggtgaa tcaggacaag gatatcatgg
     3541 ataccatgac ggtcaatatc cagtccgacc ttcccgaccc tgaggaagcc ctgataaata
     3601 accagaaaat agccactctc agaaaaattg ttgcccagct gaagtcaccc tacagggaaa
     3661 taattgaact gagatattac cgggaatatt catacgaaga aatcgctacc gaactgaaaa
     3721 ttcccatcgg aaccgtaaaa gcccagttat atcgtgccaa aacacttctt tacaatatat
     3781 ttgtaaaaac cggggcaaga taatgccgga gatcatattc aaatactttc cttcgctgtc
     3841 cgccctgcag aaggaacaga tatcaggcct gaaacccctg tatgatgatg gaacagtaaa
     3901 ataaacgtca tatcacgcag ggatatggat aatttctata tccgccacgt actccattca
     3961 atggcaatag ccagggtttt tgccttcagg ccgggaagca ggatcctgga tgccggcacg
     4021 ggcggcggct ttcccggaat acccctggca atactgttcc cggaatcgga attctccctt
     4081 ctcgattcaa ccgggaaaaa aataaaagtg gcaggcagcg ttgcagatgc actcagacta
     4141 aaaaatatca gactgataaa taaaagaatt gaggatgaaa agggaaaata cgatttcatt
     4201 tccggcaggg ctgtaactga atttaacacc ttcgtgaaac ttaccggtaa aaacctggaa
     4261 aaaaccaggg ccggcgagcc cggaaatggt atcatctacc tgaaaggagg agattgtgga
     4321 aaagaaccgg gaaaatacgg agatcaggta aaaatttggg atataaaaaa tttcttcagc
     4381 gaggatttct ttgaaacaaa aagaataatc ttccttgcag aatctgcaat caggcatccg
     4441 aaaccctgat gctccggttc ccctgcctca gccggcggaa ttccttccgc gtttcagcgg
     4501 gtttccggtt tccggcaccc ctctttttgc tgccggagag atggtttttc atataaaatg
     4561 taaaaacatt ccgtataatt catttttttc aaaaaatagc tatatttgcg gtcttaaata
     4621 aaacaaggga aaaccagatt ctgaaaaatg aaaaggactt atcagccttc cagaaggaaa
     4681 agagcaaaca agcatggatt cagagaaaga atgtctaccc ggagcggaag gcgtgttctt
     4741 tctgcccgga gggcaaaagg cagaaaaaaa ctgacagtat catcggaact cagtggcaaa
     4801 gcctgattct gatatctaaa taattacgaa aaggtgccct gcaacggcac cttttttgtt
     4861 aattctccga aaatgacccg gattcttgac catatcattg atacagacct cagggaaaca
     4921 gccttgaaag ttgcatccgg gaaacggata acaccggatg aaggcatttt gctctatgaa
     4981 aggggcgatc tgcccgttct gggcctatta tcatcaatgg tcagaaaaag gattaacggc
     5041 aattatgcct tttataaccg caattttcat attgaaccga ccaatatatg catctacaac
     5101 tgcaggttct gctcataccg gaaacctgcc ggcgacccgg aaagctggga ttacagccat
     5161 gatgaaataa tgaatatggt cagggcattc gacgggaaga atgttaccga ggttcatatc
     5221 acggggggcg ttcatcccgc tcacgatgtt cactattact gctcacttgt cgcaaaaatc
     5281 aaggctcaca gaccggagct gcacgttaag gccttttcgg ccgttgagct cgaccacatg
     5341 atcaggctgg caggattgac aactgagaaa ggactgaaac ttctgaaaga atccggcctt
     5401 gactccattc cgggcggagg tgcggaaata ttcgatgaaa agatcagaaa acagataagc
     5461 aatgaaaagg cctcttcaga aaaatggctg aacatccatg aaacggccca ccgcctgggt
     5521 atctcttcaa atgccaccat gctttacggg cacctcgaaa catggccgca caggatagac
     5581 catatgaaca ggctgcgtaa gctgcaggat aaaacaggcg ggttcaatgc attcatccct
     5641 ctgaaataca ggatggcaaa taacaggatg tctcagctgg gggaagtaag tattattgaa
     5701 gacctgagga attatgccgt tgccaggata ttcctcgaca atataccgca cataaaagcc
     5761 tactggccaa tgacggggaa aattgttgca cagctgagtc ttttctttgg agccgacgat
     5821 cttgacggaa ccattgacga caccacaaga atatattcga tggccggggc agaggaacaa
     5881 aacccgtcaa tgacagccgg ggagatctcc tcacttattg ccggggcggg ctatgagccg
     5941 gttgaaagag actccctcta ccggagggtt taaccagata ataattttat ccttacattt
     6001 gcgttatcaa taatgtacat ctgtccgtat gagcttaaag aaattcatcc tcagtaaact
     6061 gtttctaaaa cagctcggta tagccttcct gatcaccatc ggaacaatct tcatactgat
     6121 gatcagcctg aacatatata cacgtcacgg acaggcagtt ccggtacccg atttcacagg
     6181 tctcaacatg gaggaaacca aagccctggc aaaaaaatca aaaatgaaat accaggtggt
     6241 ggattccgtg tatacatccc atgttatgag aggctgtgtg gctgaacaaa atccaaggcc
     6301 gggcttcagg gtcaagaaac gaagaaccat atcattgacg atcaatgcat ttcgtccgga
     6361 aatggtggct gttcccgatc ttcttgacct gcccttaaga caggcgatct cactgataga
     6421 aagtaccgga ctggtaatgg gaacattgcg ctacaaaccc gatctttcgg taaatgtcgt
     6481 tctgagccag ctattcaatg gcaaggaaat cactccgggt gattcgattc agaaaggttc
     6541 ggtaatcgac cttgtcctgg gcaaaggcct cagcaatcag aggactgcgg tacccaacct
     6601 gataggcctg ccccttgagg atgctgccgg cagaatactc ggggcctccc tgaatctggg
     6661 cacatttatt tttgacagga cagtacgtac gggagaggac agcctgagag ccttcgtata
     6721 caaacagaat cctgaatacc gtgaggatgc cacgctgcag ctgggatctt ccatctacct
     6781 gtggatgaca accgattcac tcaagctgcc cgccgactct gcatttgtca gagccgacac
     6841 tacaatgaca acggctgtat tagcgggaaa ggtacattaa tcctgatgat cagaagaaat
     6901 ctactgaata ttgcaatcct cctgagcttc acaggaaatc tgacgggcca ggaaattgtt
     6961 atcggcctta cctcaaatcc ggtactgaaa aatacccctt cacggtctgc aaaatctttt
     7021 tcagccgaac aactgacact tccgttcttc gatgatttct ccggaagaga tatttatccc
     7081 gatcccgcaa aatggagtga cgataatgtg tttataaaca atacatattc ccgggatcag
     7141 atcacaacag gaattgccac atttgatgca ctcgacagaa caggcagact atacggggaa
     7201 gcatctccgg taacattcag ggccgatgtc ctgacttcac aacccataga ccttgcctac
     7261 acagcggctg acaatatccg gctcagtttc ttttaccagc cgggcggcat aggtgacatg
     7321 cccgagaaaa atgacaccct caccctccat ttcttcgcac ctgacgaaaa taagtggtat
     7381 tcggtatgga gagcctacgg cgattcttca gggatattca gactggccat gctgaaaata
     7441 gatgatccca ggttccttaa aacgggcttc cggttcaggt ttataaatta tgccagtctt
     7501 agccgcagca aaaccgacct ttccatgaca ggcaactgtg accactggaa tatagactat
     7561 gtactgctgg ataaaaacag aagcagcaga gataccattt tccacgatgt ggcattcagg
     7621 aaaccattcc ggtcgcttct aaaaaatcac gaggccatgc cacccgcaca tttcaacaag
     7681 atataccttc aggaaatggg ggataacatc ccgataagct accgtaacaa cgacaccatt
     7741 gtcaggaatg tcacaagaaa cttcagaata tgggatgtct acaaaaatga ggaagcaata
     7801 tctttctcag ccggagctgc aaatgtcggt cctcttgaga atgttgacta caatgccagc
     7861 ctgttctata ctttcaacac aataggccgg gattccgcgc tcttcaagat aaacgcatgg
     7921 cttaagaccg acgattttga tcccaaaaca aacgataccg ttacctattt ccaggtattc
     7981 agaaactatt ttgcctatga cgacggttcg gctgaggcag gttacggcat taacggcctc
     8041 ggatcgagaa atgcaatggt tgcggtgagg ttcagggcct acacccggga tactctcaga
     8101 gccataagca tctgtttcaa tgacagctat atgcgggcta accggagaag cttcagtctc
     8161 atggtatgga acgacaacaa cggcctgccg ggcgatgtta tctatacaag acaggaaaca
     8221 atggttgagc aggcagggga tataaacggc ttccatacat attttctgcc ctcgggtgtt
     8281 gccgtggagg ataatttcta tgtcggatgg aaacagcagt cagaaacctt tctcaatgcc
     8341 ggattcgacc tcaacacacc ccacaggggc aatctgctgt attggataaa cggcagctgg
     8401 ttcacctccc agcattccgg tactctcatg ataagacccg tggtcgggcc tcctgtcaga
     8461 acatccgttc atgatgttta ccataatccg gaaacgcctc tgcgcttcca tcccaatcct
     8521 gcaagggatt atatatcctt cgacagaaag gatctgacag aaacggacca gtatgatgtt
     8581 tccgtttttg atctcaacgg gagggagctt ctgaaagtga aaatgacgga taaaacagat
     8641 atctcctcat tgaaaccggg tgcctacatt attgttcttg gcaggaacgg gaaagcggtg
     8701 ggatacagcc gcctcatcaa aataaagtaa tacccggaat gtcggaggaa gaaatagccg
     8761 aaaaacagga actgtttgaa cattacaggt ttaatgttga tcccgggcag tctctcctga
     8821 ggatagataa atatctgttt tcacgtattg agaacacctc gaggacaagg atccggaatg
     8881 ctgccgatgc aggaaatatc ctggtgaacg atatacccgt cagggcaaac tacagggtga
     8941 agccggatga tattgtacgg atcgttctgc ccgatccgcc gagggagatc gaactgatcc
     9001 cggaagacat tcccattgat atcatttatg aggacgatga cgtgatcgtg gtcaacaagg
     9061 cagcaggtat ggtagtccac ccggcttacg gcaattacag cggaacgctg gtcaatgccc
     9121 tcatgtggca tttcagggat ctgccgctct tcgcaacagg tgaactgagg ccgggtctcg
     9181 ttcacagaat agacaagaac acatcgggcc ttctcgttgt ggccaagaac gagatttccc
     9241 tcaacaggct ctcaaggcag ttctacgaaa agacggcgga ccgcatctat accgcgattg
     9301 cctggggtgt acctgctccg gcagaaggca cggtcacggg acatatcggc aggaatatac
     9361 gcgacaggaa aataatgcag gtctttcccg acggaagccg gggaaaacct gccatcaccc
     9421 actataaggt tctcgaaaac ctgggatata tttcacttat cgaatgcaga ctcgaaacag
     9481 gacgaacgca tcagataaga gtacatttca gttatatcgg gcaccccctc ttcaatgatg
     9541 atgaatacgg gggtgacagg attcttaagg gtacgacgtt ccaaaaatac aggcagtttg
     9601 taagaaactg cttcagcctg ctgccgcggc aggcacttca tgccagattt ctgtcattca
     9661 gccatcccgt aacaggtaaa aggctgtctt ttgacacccc gatgccggac gacatgcagc
     9721 aggtaatcac acggtggaga gaatacatat cgggaaggga tgcatagaat ttgtaatttt
     9781 gacctgaaac cagcctaata atgaaaacca ttgcagtagt agccggcggt gactcatcag
     9841 aataccagat ctccgtaaag agcggaaaat cggtcgccgg aataatctca tccctttaca
     9901 gagtctatct gataatcatc aggggcagca actggtactg ggaggatccc aaagggagat
     9961 accacaccgt cgacaagaat aacttcaccc ttaaagctga tgatctgacc atcaggtttg
    10021 atgccgtctt catcgccatc cacggcaaac ccggtgaaaa cggcatgctg cagggctatt
    10081 tcgacatgct tcagattccc tatacaggga gcagctcatt ctgttccgcc ctcactttca
    10141 ataagcatgc atgcaagctg ttcctgaagg aatacaatat aggaatggcc gaaagcattt
    10201 ttttaagaaa gaacgagaag ccggacaatg ctgtcattgt aaacaaactg ggactgccat
    10261 gttttgtaaa acccaacgac agcggttcga gtttcggggt ttcaaaggtc agacaggccg
    10321 gggaactggc tgatgccata tcagaggcat tcagggaaag cgatgagata ctgattgaat
    10381 cattcatgca gggcagggag gttgcctgcg gcctggtgat gacaaaatca cggaaaataa
    10441 tcctgcccct taccgaaata ataagcaaaa atgaattttt cgatttcgag gcaaaatata
    10501 ccccggggaa gtctgatgaa attacacctg ctgaccttcc ggagaaactg actgatgaga
    10561 tccggaagct gagctcccgg atagctgacc tgctcggctg cagtggaata gtccgggtcg
    10621 atttcataat tgtcggcaat aagcctcttt tcctggaaat caacacagta cccggaatga
    10681 cagaagaaag catcattccc aaacaggcag cagccgccgg aattcccctt aaggacctgt
    10741 actcgatggt gatcgaggac ttgtttgtct aacaacactg ccctgaatca cctgatgaat
    10801 cacctgtaat tccctccacg ggcgcaaaag caaagaaccc ggcttccgcc ccggaatggg
    10861 aatggcatta ataccgggaa accgggcgct tacttttctc tggccttttt cagtgcaccg
    10921 gtag
//
LOCUS       DAOSPX010000070        10877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5924_length_10877_cov_4.133802, whole genome shotgun sequence.
ACCESSION   DAOSPX010000070 DAOSPX010000000
VERSION     DAOSPX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5924_length_10877_cov_4.133802"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="PLZ75_05145"
     CDS             <1..1301
                     /locus_tag="PLZ75_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOS71816.1"
                     /translation="RLDLTSKLTDASEILSKLTDATPETEDTTALALIKIPENIDFGF
                     NAAISNFIYGKIRANNVRGHILVKDGVLSMKETGMDIMGGKVSLNADYDTRDTLRPLM
                     KAGFSIRGIGIRDGFETFNTIQKFAPAASGVDGKVNLDLSFESLLGKDMMPLVSTIMG
                     EGKLQSDQVTLIESAAYDRMKELLKLGENYTNTFRDLNLSFSVRTGRVYVNPFDVKAG
                     NIRMNISGDQGIDQTMNYLIKTEIPRSELGGSVNSFIDGLSAQASALGFAFKVPADVM
                     KINVKLTGVFGKPIVTPVFGSGPGESTPGLKSAAADAVKEGVAGAVSEGREKLIKEAE
                     ERGDRLIREAETRAQQLRDEAAKSAEAIRKEADIQAQKISDEAASKGAVARLAARKTA
                     ETLRTEADKKAELLIRQADEQASKIIDEARARKEDLLDKI"
     gene            complement(1317..1664)
                     /gene="rplS"
                     /locus_tag="PLZ75_05150"
     CDS             complement(1317..1664)
                     /gene="rplS"
                     /locus_tag="PLZ75_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOS71817.1"
                     /translation="MNLMNVVDKELAVKNDFPKFQAGDTITVHYKIIEGNKERIQQFR
                     GVVLQRAGSGHTATFTVRKMSGNIGVERIFPVSSPFISKIEINKHGKVRRARIFYFRD
                     LTGKKARIKERKF"
     gene            complement(1782..3503)
                     /locus_tag="PLZ75_05155"
     CDS             complement(1782..3503)
                     /locus_tag="PLZ75_05155"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOS71818.1"
                     /translation="MRKFIPIVLTVFSGLLFSLHPAEACTNFIVTKGASQNGSVMITY
                     SADSHVLYGELYYRPAREYPAGAMMDVYEWDSGRFLGRIRQAPRTYSVVGNMNEFQLS
                     IAETTYGGIGSLSDPDGILDYGSLIYITLQRARSAREAIKVMSELLAEYGYASSGESF
                     SIADKNEAWIFEMIGKGPGNKGAVWVARLVPDGYVCAHANQARIRTFPLATGKKNSIS
                     ITSKDLSRIFDPLVECVYAWDVIEVARAHKLYSGADKDFSFSDTYNPVDFSGARFCEM
                     RVWSFFRALHSDMDKYENYAAGHDLENRMPLWIKPERKISSYDLMNFMRDHLEGTAFD
                     MRKDIGAGPFGSPYRWRPLTWSVSDGPDSPVYCNERATATQQTGFVFVTESRNWLPDP
                     VGGILWFGVDDASTSVFNPMYCGITEIPECFRVGNGDLVTYSPTSAFWLFNLVANMCY
                     SRYDVMSEDAIKVQKRLELGYIAETAEIDDKAAKLYEKDVWEARDFLTAYSLKTAKNT
                     FDEWKTLSEYLLVKYIDGNIKREKNGKFEVSPDNYPLMPDQPGYPDSWKKSVIRDTGD
                     KLRVPED"
     gene            complement(3572..4318)
                     /locus_tag="PLZ75_05160"
     CDS             complement(3572..4318)
                     /locus_tag="PLZ75_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOS71819.1"
                     /translation="MNYKLEICVDSVESAINAQKGGANRVELCDNLPEGGTTPSYGTI
                     LSARQNLDILLHVIIRPRGGDFLYSDREYEIMKRDIEMCGEIGADGIVIGLLNADGTV
                     DVERTQQLVKIADTMNVTFHRAFDMCSDPMRGLEDIILTGATRILTSGQKDTVPQGVN
                     LISTLVKQAGDRITIMPGSGLDEYNIADMARATGAREFHLTGRKIVESGMKFRREGIP
                     MGGFPDIPEFSRKVADPERIRKIINILGSL"
     gene            4391..5293
                     /locus_tag="PLZ75_05165"
     CDS             4391..5293
                     /locus_tag="PLZ75_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOS71820.1"
                     /translation="MKPTLLVLAAGMGSRYGGNKQLDEVGPSGETIIDYSIYDAIRAG
                     FGKIVFIIRRDLEAQVKERFFEKLKNKIELDYVFQELTNVPEGSKIAPDRVKPWGTSH
                     AVLVAENSIREPFGIINADDFYGYDSLKILHDFLVNDKDPATYCIVGYKLGNTLSDHG
                     YVNRGVCNVDSDGLLQNIVEVLKIEKTEDGAKAPSSDGGEQLFTGSEIVSMNLFGFKP
                     GCFNILKEDFSKFIDEHGMDARSELLIPNTLDRYIKKGDIRIKVLMSNDRWFGVTYRE
                     DKPFVVENIRKMVQEGIYPEKIYQ"
     gene            complement(5311..6987)
                     /locus_tag="PLZ75_05170"
     CDS             complement(5311..6987)
                     /locus_tag="PLZ75_05170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS71821.1"
                     /translation="MSEDTPENKNLFISPVRIPLSLSANFAELRADHFHSGIDIRTQG
                     VIGKEIVAAADGYVYRISVSPGGFGKAIYLRHPSGYSTVYGHLDRFVTEVEEYVTDYQ
                     YQKKSFTVSIFPPKDKFPFRQGEIIAYSGNTGSSGGPHLHYEIRKSDSEIPVNPLLFE
                     FGAGDDIEPILERLAIYPLSGNTRINGVHAVKKINLAGGHGNYYIPTENEITISGPAG
                     FGIKSYDLLNDSYNKCGIYSTELKIDGKTIFRNVMDEFSFNESRYINSHIDYETWIRE
                     SIRFERTFLLPNNRLSTYQDIVNRGIYNFSEDRIYHVSLELRDVHDNISVLAFKVKGS
                     PAGAPDDSYTSEPGLVVMPYNRSNRFRAGNIAVSIPEGALYDTLMFRYRKEPSKNGML
                     SELHYVHDRATPLHKSYSLSIKPLAIPEGKESKLLIVNLRDDKTRIPLSGRYSEGFVN
                     GQSSVFGAFYIGIDTVPPLVSAVGFSNGADLSGRQEMRIRISDNLSGIKSYEPFIDGN
                     WALFEYDQKNRVLIYKFDPRRIKKGTNHSLTLKVSDNRDNITTFTTGFRW"
     gene            7116..7448
                     /locus_tag="PLZ75_05175"
     CDS             7116..7448
                     /locus_tag="PLZ75_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71822.1"
                     /translation="MINNDYFCSKDSDMSDSVYSDLKRLNKKLDELLSTYENLRSEFQ
                     DLEKNNEELRMVLHEREERIKELEIKYERVKLSGALLGDTGNTAEAKRKITELVREID
                     RCVALLNR"
     gene            7458..7763
                     /locus_tag="PLZ75_05180"
     CDS             7458..7763
                     /locus_tag="PLZ75_05180"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOS71823.1"
                     /translation="MDDKLSIRVNVADRYYPLKVERENEEKIRRAARMINEKVLQYKQ
                     RYTDKDVQDFLAMAALQYVIKLTEQEDRLERDYIPEAIQELIQKIDSALEKKSDSVL"
     gene            8010..9572
                     /gene="rny"
                     /locus_tag="PLZ75_05185"
     CDS             8010..9572
                     /gene="rny"
                     /locus_tag="PLZ75_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOS71824.1"
                     /translation="MTLLITTVSSLLTIILSAAALVTGLGIGFLAWHTALKNKSRKII
                     SEAEAEAEVIRKEKILQAKERFLQLKTEHEKVINEKNNKIAQSENRIRQKELTLSQKI
                     EEAQRRKNEADAIRENLNAQLEIIDKKNEELEKLHRQQVEKLEIISGLSAGEARDNLI
                     ESLKEEAKTGAMSYINEILDEAKMTANKEAKRIIIQTIQRVAAENAIENAVTVFHIDS
                     DEIKGRIIGREGRNIRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLALHQLVTD
                     GRIHPARIEEIVEKTKKQIEEEILETGKRTAIDLGIHGLHPELIRLVGKMKYRSSYGQ
                     NLLMHSREVANLASIMAAELGLNPKLAKRAGLLHDIGKVPDDEPELPHAILGMKIAEK
                     YKEKPEICNAIGAHHDETEMTTLIAPVVQVCDAISGARPGARREVVESYIKRLKELES
                     LALQYPGVTKTYAIQAGRELRVIVGAEKVSDKDAEGLSFDIARKIQNEMTYPGQIKIT
                     VIRETRAVNYAK"
     gene            9653..10807
                     /gene="wecB"
                     /locus_tag="PLZ75_05190"
     CDS             9653..10807
                     /gene="wecB"
                     /locus_tag="PLZ75_05190"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOS71825.1"
                     /translation="MRLMNIVGARPQFIKASAISRAIKLNFPDKISEIIVHTGQHYDK
                     EMSDVFFDELDISKPHYNLAVGSSRHGKQTSMMISGIEEVLLGEKPDCVVLYGDTNST
                     LAGAIAAAKLHFPVIHIEAGLRSFNKTMPEELNRIISDHASTLLFAPTNSAFKNLMNE
                     GFRPENSPPYTIDNPKIYLTGDIMYDNALFYAELAERKKSGFHKSIQVKREEFVLVTI
                     HREINTDDISRLETLMLTLNALARDHSIPFVMPLHPRTTLSLRTRLESLYNDLRSSKY
                     IRLIPPVSYLEMILLEKTSRLIITDSGGVQKESHFFRKPCLVLRAETEWVELVKNGTA
                     ELVDADPEKLRNGFRKFTDDGKELEYPAFYGNGKTAEFILNEIIMMFEKP"
BASE COUNT         3225 a         2335 c         2461 g         2856 t
ORIGIN      
        1 gcagactgga tcttacatca aaacttacgg atgcatcaga gatattgtca aaacttacgg
       61 atgccacacc ggaaactgaa gataccaccg ccctggcact cattaaaata cccgaaaaca
      121 tcgacttcgg tttcaatgcc gcgatcagca attttattta cgggaaaatc agggcaaata
      181 atgtcagggg ccatattctt gttaaagatg gtgtcctcag catgaaggaa acaggaatgg
      241 atatcatggg agggaaagtg tcgctgaatg ccgactatga cacccgggat actctgaggc
      301 ccctgatgaa ggcaggcttt agcatcaggg gaataggcat acgtgacgga tttgaaacat
      361 tcaacacgat tcagaagttt gctcctgctg ccagtggagt tgatggcaaa gtgaatctgg
      421 atctttcatt tgaaagttta ttgggtaagg atatgatgcc tctggtgagc accattatgg
      481 gagaaggaaa acttcagtct gaccaggtca cactgattga gtctgccgca tatgacagaa
      541 tgaaggagct tctgaagctg ggagaaaatt ataccaatac attcagggat ctcaacctga
      601 gtttttctgt caggaccgga cgggtgtacg taaatccctt tgatgtgaag gcgggcaata
      661 tacgaatgaa tatcagcggt gatcagggaa ttgaccagac aatgaattat ttgattaaga
      721 cagaaattcc gagatcggaa cttggcggat cggtaaattc ctttattgac ggattgtctg
      781 cccaggcctc cgccctcggc tttgcattta aagtgcccgc tgatgtgatg aagatcaatg
      841 taaaacttac cggagtattt ggaaaaccga tcgttactcc tgtatttgga agcggtccgg
      901 gggaaagtac tccggggctg aagtcagcag ctgctgatgc agtaaaggaa ggtgttgccg
      961 gagctgtcag cgaaggcagg gaaaaactga taaaagaagc agaggagcgt ggtgacaggc
     1021 tgataaggga agccgaaaca agggctcagc agctgcgtga tgaagctgca aaatctgcag
     1081 aggcaatccg gaaggaagcc gatattcagg cacaaaagat aagcgatgaa gctgcatcaa
     1141 agggtgccgt tgccagactt gctgcacgga aaactgcaga aaccctgaga acagaagcag
     1201 acaaaaaagc tgaactttta atcagacagg ctgatgaaca ggcatcaaaa ataattgatg
     1261 aagccagggc aagaaaagag gatttattgg ataagatctg agaaacaaaa atcaaatcag
     1321 aattttcttt ccttaatcct tgccttcttg ccggtaaggt cgcgaaaata aaatatcctg
     1381 gccctgcgga ctttcccgtg cttgttgatt tctatcttgc tgatgaaggg ggatgagaca
     1441 ggaaaaattc tttcaacacc aatgttgcct gacatttttc tgacggtaaa agtagcggta
     1501 tgcccgctgc cggccctttg aaggacaact cccctgaatt gctggatcct ctccttgttg
     1561 ccctcaatga tcttatagtg tacggtaatt gtatctcctg cctggaactt gggaaagtca
     1621 ttctttactg ccagttcttt atcaacaaca ttcattaggt tcatctcgga tatataaaaa
     1681 ttgcccgcaa atctattaaa acaaattcaa aattcaaaat ccggtaatca ttgttctttc
     1741 tctttctgaa tattgtcctt ttctcctgca gatcacggtc cttaatcttc cggaacacga
     1801 agcttgtcgc cggtatccct gataacggat tttttccacg aatcaggata acccggctgg
     1861 tcaggcatta agggataatt atcgggacta acttcaaatt ttccgttctt ctcccttttg
     1921 atatttccgt caatatattt tacaagaaga tattcactca gtgttttcca ttcgtcaaag
     1981 gtattttttg ccgttttcag agaatatgcc gtaagaaaat cccgggcttc ccatacatcc
     2041 ttctcatata attttgcggc cttatcatct atttctgccg tttcagcaat atagccaagt
     2101 tccagccttt tctgcacctt tattgcatcc tcgctcatca cgtcgtacct tgaatagcac
     2161 atatttgcca caagattgaa aagccagaag gcggaggtcg gagagtatgt gaccagatcg
     2221 ccgttgccaa ccctgaaaca ttcgggaatt tcagttatac cgcagtacat cgggttaaag
     2281 acgcttgttg atgcatcatc aaccccgaac cataaaatac ctcctacagg atcgggaagc
     2341 cagtttcggc tttcggtaac aaacacaaat cccgtctgtt gtgtggctgt ggccctttca
     2401 ttgcaataaa ccggtgaatc cggtccgtct gataccgacc atgtcagagg gcgccatctg
     2461 tatggcgatc cgaacgggcc tgcgccgata tcctttctca tgtcaaaggc tgttccctca
     2521 agatggtcgc gcatgaaatt cataaggtca tagctggaaa tcttacgttc tggcttgatc
     2581 catagaggca tacgattttc aagatcatga ccggcagcat aattctcata cttgtccata
     2641 tcactgtgca gagccctgaa aaatgaccac actctcattt cacagaatct ggcacctgaa
     2701 aaatcgaccg ggttataagt gtcagagaaa ctgaaatcct tgtcggcacc gctgtacagt
     2761 ttgtgtgccc tggcaacttc tattacatcc catgcataaa cacattcaac aagcggatcg
     2821 aatatcctgc tgagatcctt tgaagtgatg gatatactgt tcttctttcc tgttgcaagg
     2881 ggaaatgtcc ggatacgtgc ctgattggca tgagcgcaga cataaccgtc gggaaccaga
     2941 cgggccaccc atacggcacc cttgtttccg ggacctttcc cgatcatctc gaatatccag
     3001 gcttcattct tgtcggctat tgaaaatgat tcacccgagc tggcatatcc gtattcggcg
     3061 agaagttccg acatcacttt tatggcttcc ctcgcacttc tggctctctg cagggtgatg
     3121 tagataaggc ttccgtaatc aagtattccg tcaggatctg acagtgaccc tatccccccg
     3181 tatgttgttt cggctattga aagctgaaat tcattcatat tgcctactac cgaatatgtg
     3241 cggggagcct gtctgatcct tcccagaaat ctgccggaat cccattcata gacatccatc
     3301 attgcaccgg ccggatactc ccgggccgga cggtaataaa gctctccgta cagaacatgt
     3361 gagtctgccg aataggtgat cattactgac ccgttctgcg aggctccctt tgttacgatg
     3421 aaatttgtac aggcctctgc cggatgcagg ctgaataaaa gtccggaaaa tactgtcagg
     3481 actattggaa taaattttct cattgtatgc ttgtattata aaatttacag ccgggaggct
     3541 tgcttttgca agcgcagttc tccggaataa tttacagaga acctagtatg tttatgattt
     3601 ttcttattct ttcaggatca gcaaccttcc tggagaattc cggtatatcc ggaaatcctc
     3661 ccatgggtat cccttcacgc cggaatttca tgccgctttc cactatcttc ctgcctgtca
     3721 gatgaaattc ccgggcccct gtagccctgg ccatatcagc aatattgtac tcatcaaggc
     3781 cgcttccggg cattattgta attctgtcgc ccgcctgctt cacgagagta ctgataaggt
     3841 tcactccctg gggcacagtg tccttctgac cggaggtcag tatccgtgtt gcacccgtaa
     3901 gaataatatc ctcgagccct ctcatgggat ctgaacacat atcaaaggcc ctgtgaaatg
     3961 taacattcat tgtgtcagca atctttacca gttgttgagt ccgttccaca tcaaccgtgc
     4021 catcagcatt cagaagtccg attactattc cgtccgcacc gatttcaccg cacatttcaa
     4081 tatcccgctt cattatttca tattcccggt cactgtagag gaaatcaccc cctcttggcc
     4141 ttattattac atgaagcaga atatcaagat tctgtctggc actgagtatt gttccgtaag
     4201 agggagtggt acccccctcg ggaagattgt cgcaaagttc cacgcggttt gcgccgcctt
     4261 tctgtgcatt gattgctgat tcgacgctgt caacgcaaat ctcaagttta taattcatct
     4321 tacttttttt aaaagtgtta aaaataacat tttttttagt tattattgtg cagaccaaaa
     4381 tccattaaat atgaagccta cacttttagt tcttgcagca ggaatgggaa gccgatacgg
     4441 tgggaacaaa cagcttgacg aagtcggacc ctcaggtgaa acaatcatcg attattcaat
     4501 ttatgatgct atcagagccg gttttggcaa gatcgtcttt atcattcgac gtgatctgga
     4561 agcccaggta aaggagaggt tttttgaaaa gctgaaaaac aaaattgagc ttgattatgt
     4621 cttccaggaa ctgacaaatg tcccggaggg ttcaaagata gcacctgacc gtgtaaagcc
     4681 ctggggtacc agtcatgcag tcctggttgc tgaaaacagt atcagggaac cgtttggaat
     4741 aataaacgct gatgattttt atgggtacga ttctctgaag atacttcatg attttctggt
     4801 aaatgacaag gatccggcta cttattgcat tgtgggctat aaactaggaa acaccctgtc
     4861 cgaccatggc tacgtgaacc ggggtgtatg caatgttgac tcagacggcc ttctgcagaa
     4921 tattgtagag gtactgaaga tagaaaagac ggaagacggt gccaaagctc cttcttctga
     4981 cggaggggaa cagctcttta ccggcagtga aatagtctcg atgaatctgt tcggattcaa
     5041 acccggatgc ttcaatatcc tgaaggaaga tttcagcaag tttattgatg agcatggcat
     5101 ggatgccagg tccgaactgc ttatcccgaa taccctggac agatatatta aaaagggaga
     5161 tatccgcatt aaggtcctga tgagtaacga cagatggttt ggtgtaactt accgtgaaga
     5221 caaacccttt gttgttgaaa atatcaggaa gatggtgcag gaaggaatat atcctgagaa
     5281 gatctaccag taaacaggga atagcaggct ttaccaccgg aagccggtgg taaaggtggt
     5341 gatattgtcc ctgttgtcgc ttaccttaag tgtaagagaa tgatttgttc ccttctttat
     5401 tctccgggga tcaaatttat atatcagcac cctgtttttc tgatcatatt cgaataaggc
     5461 ccagttgccg tcaataaacg gctcatatga tttaatgccg gataaattat ccgagatcct
     5521 tattctcatt tcttgccggc cggagagatc ggctccgtta ctgaagccga cggctgaaac
     5581 aagcggagga actgtatcta taccaatata aaatgccccg aatactgaag actgtccgtt
     5641 tacaaagccc tcagaatacc tgcccgacag gggaattctt gttttatcgt ccctgagatt
     5701 tactatcagc agcttggatt cctttccctc aggtattgcc aggggtttta ttgaaaggga
     5761 atatgacttg tgaagcgggg tcgccctgtc atgaacataa tgaagttccg ataacattcc
     5821 gttttttgac ggttctttcc tgtaacggaa catgagagtg tcatatagag ctccttcggg
     5881 aatggaaacg gcgatgttgc ccgcacggaa cctgttcgac cggttgtaag gcattacaac
     5941 aagtccgggt tcggatgtgt aactgtcatc cggggcaccg gccggacttc cttttacttt
     6001 aaaagccagg acagaaatat tgtcatgaac atcccttaat tcaagggaaa catgatagat
     6061 tctgtcctct gagaagttat agattccgcg gttgactata tcctggtatg tgctcagccg
     6121 gttgttgggc agcaggaagg ttctttcaaa gcggatactt tcccttatcc aggtttcata
     6181 gtcaatatga ctgttgatat atctcgattc attgaatgaa aattcatcca taacattacg
     6241 gaaaattgtc ttgccgtcaa tcttcagttc tgttgaatag attccgcatt tgttataact
     6301 gtcattcagt agatcatagg attttatgcc gaatccggcc ggaccggaaa ttgttatttc
     6361 attctcagtc ggtatgtaat aatttccatg cccgccggcc aggtttattt tcttaactgc
     6421 atgcactccg tttatgcgtg tgtttcctga aagaggataa atggcaagtc tttccagtat
     6481 gggttcaata tcgtcccctg cgccgaattc aaaaagaagg ggattaacag gaatctccga
     6541 atcggatttt cttatttcat aatgaagatg gggaccgcct gagctgcctg tattccctga
     6601 ataagctatt atttcaccct gtctgaaggg aaatttgtct ttaggcggaa agatggatac
     6661 agtaaagctc tttttctgat attgataatc agttacatat tcctccacct cggtcacaaa
     6721 gcggtccagg tgcccgtaaa cggtggaata tcctgacgga tgcctcaggt aaatggcttt
     6781 gccaaaccct ccgggcgata cgctgattct gtaaacatat ccgtcagctg ctgccacaat
     6841 ctccttgccg atgactccct gggtcctgat atctattccg gaatggaaat gatccgccct
     6901 gagttctgca aaattagctg ataatgaaag cggaatccgt accggtgaga tgaacagatt
     6961 cttattttcc ggtgtatcct ctgacaaacc ctggagaaac agcagaaata ctaaaattat
     7021 caaaacaggc atggtattgc attttccgtt tatgttttgc aaatctatat atccggagca
     7081 cttgtgcaaa ctatttaaat caaatttttg aaaaattgat caacaatgat tatttttgca
     7141 gtaaagattc tgatatgtcc gatagtgttt attcagattt aaaacgcctg aacaaaaagc
     7201 tggatgaact gttaagcact tatgaaaatc tcaggtcaga atttcaggat cttgagaaaa
     7261 acaatgaaga actgaggatg gttcttcatg aacgtgagga aagaatcaag gaacttgaaa
     7321 ttaaatacga aagggttaaa ttatcaggag cactgttggg agatactgga aacacggcgg
     7381 aagcgaagag gaaaataacg gaattagtgc gggaaataga cagatgtgtt gctcttttaa
     7441 acagataaaa tataacaatg gatgataaac tatcgatcag ggttaatgtt gccgacagat
     7501 attatccatt gaaagtggaa cgggaaaatg aggaaaagat caggagagct gccaggatga
     7561 ttaatgagaa ggtgttgcag tacaaacaac gctacaccga caaggatgtc caggattttc
     7621 tggcaatggc agccttgcaa tatgtgatta aactcacaga acaggaagac aggcttgaaa
     7681 gagattatat cccggaagcc atacaggagc tgattcagaa gattgattct gctcttgaaa
     7741 aaaagagtga cagcgttctt taaaatagca aataactgcc cgcattgttt cttcaatcat
     7801 ttttgaaact caacagttaa aactttgaag tctaccttga actgacaagg acaggcctta
     7861 tcccgattcg tcgggatcac actccggtgt gacactggaa gtccgacccg gatcaggaac
     7921 ttcaaccatg tagacatggg gttttataaa atctgaggga actctgcggg tttttttttt
     7981 atagactata aacacaatta cagaaaaaca tgacattatt aatcaccacg gtcagcagcc
     8041 tgctaaccat tatattaagc gctgctgcac tggtaacagg attggggata ggattccttg
     8101 cctggcatac ggccctgaag aataaaagca ggaaaatcat aagtgaagca gaagcagaag
     8161 ctgaagtaat cagaaaagaa aaaatccttc aggcaaaaga aagatttctt cagcttaaga
     8221 ccgagcatga aaaagtaata aatgagaaaa acaacaaaat tgctcagtca gaaaacagaa
     8281 taagacagaa agaacttaca ctttcccaga aaattgagga agcacagcgc aggaagaatg
     8341 aagctgacgc aatacgcgaa aacctgaatg cacagcttga aataattgat aaaaagaatg
     8401 aagagcttga gaagcttcat cgccagcagg ttgaaaaact ggaaattatt tccggattat
     8461 ctgccggaga agcaagggat aacctgatag aatcccttaa ggaagaagct aaaacaggtg
     8521 caatgtcata catcaatgaa atacttgatg aggcaaaaat gaccgcaaac aaggaagcca
     8581 agagaataat catccagaca atccagagag ttgcagctga aaatgccatt gaaaatgcgg
     8641 ttactgtctt ccatattgat tcggatgaaa taaaaggccg cataataggg cgtgaggggc
     8701 ggaatatcag ggctctggag gcggcaaccg gtgttgagat aattgttgat gatacaccgg
     8761 aagcaattgt tctttcggcc tttgatccgg taaggaggga aatagcacgg cttgcactgc
     8821 atcagcttgt taccgacggc aggattcatc cggcaaggat tgaggagatt gttgaaaaaa
     8881 ccaagaaaca gattgaggaa gagatccttg aaaccgggaa acggacggca attgaccttg
     8941 gcattcacgg tctgcatccc gaactgatac ggctggtcgg aaaaatgaaa tacaggtcct
     9001 catacggtca gaacctgctt atgcattcac gtgaggtagc caaccttgcc tcaataatgg
     9061 ctgctgaact cggactgaat cccaaacttg caaaaagggc cggattgttg cacgacatcg
     9121 gcaaggtgcc agatgatgaa cccgaactcc cgcatgcaat actgggaatg aaaatagctg
     9181 aaaaatacaa ggaaaaacct gaaatatgca atgcgatcgg cgctcatcac gatgaaacgg
     9241 agatgacaac gcttatagca cctgtggtac aggtatgtga tgccatctca ggagcccgcc
     9301 cgggagcacg ccgcgaagtg gtggaatcat atatcaagag gctcaaggaa cttgaatccc
     9361 tggccctcca gtatcccggt gttacgaaaa cctatgccat tcaggcggga agggaactcc
     9421 gcgtaatagt tggtgcggaa aaagtatccg acaaggatgc cgaaggactt tcattcgata
     9481 ttgcaagaaa aatacagaat gaaatgacct atcccggaca gataaaaatt acggtaatcc
     9541 gggaaacacg tgcggtcaac tacgctaaat gacagtcggt caaaaaacta ttgatgaata
     9601 ggatttatta tgctaaattt gttttccata ttcacggaaa gcaatgtcac atatgagact
     9661 gatgaatatt gtcggggccc ggccccagtt cattaaagct tcggcaataa gcagggcaat
     9721 aaagctcaat ttccctgata aaataagtga aataatagtc catacagggc agcattatga
     9781 taaggagatg tcagatgttt tctttgatga acttgacatc agtaaaccgc attataatct
     9841 cgctgtaggc tcatcgcgcc acgggaagca gacatcaatg atgataagcg gcatagaaga
     9901 ggtgcttctc ggagaaaaac ccgattgtgt ggttctgtac ggagatacca actcaacact
     9961 ggcaggggct atagcagccg ccaagcttca ttttcccgtg attcacattg aagccggact
    10021 gagatccttc aataaaacaa tgcctgaaga attaaaccgc ataatcagtg atcatgcatc
    10081 aacccttctt tttgcaccga caaattccgc attcaaaaat ctgatgaatg aaggattcag
    10141 accggaaaac tctcctcctt acactattga caatccgaaa atatatctga cgggtgacat
    10201 tatgtatgac aatgccctct tttatgccga actggctgaa agaaaaaaat caggctttca
    10261 caaaagcatt caggtgaaaa gggaagaatt tgtacttgtt accatccacc gcgaaataaa
    10321 cacggatgat atctcccggc ttgaaaccct tatgctgacc ctgaatgcac tcgcccgtga
    10381 tcattccatt ccctttgtga tgccgcttca tccccggaca accctgtctc tcagaacaag
    10441 gctcgaatca ctttacaatg atctcaggag cagtaagtat atacgcctta tccctcctgt
    10501 atcctatctc gaaatgatac ttctggaaaa gacttcacgg ttgataataa cagattcagg
    10561 aggtgtgcag aaagaatctc attttttccg gaagccctgc cttgtcctca gggccgaaac
    10621 cgaatgggtt gaactggtaa aaaacggaac agcagagctt gttgatgcgg atccggaaaa
    10681 attaagaaac ggattcagga aatttacgga tgatggtaaa gaacttgaat atcccgcatt
    10741 ttacggaaac ggaaagacag cagaattcat tctgaacgaa ataataatga tgtttgagaa
    10801 accgtgagaa attctgagaa atcctgagaa accaactctt ttcaaatgag gcatatggaa
    10861 cgagtgataa agaataa
//
LOCUS       DAOSPX010000071        10868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5929_length_10868_cov_3.877647, whole genome shotgun sequence.
ACCESSION   DAOSPX010000071 DAOSPX010000000
VERSION     DAOSPX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10868
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5929_length_10868_cov_3.877647"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLZ75_05195"
     CDS             <1..746
                     /locus_tag="PLZ75_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HOS71826.1"
                     /translation="EGFYVLSFGTDFIREYKLVKGEAFGDVYAKGFQRDANGNVIINQ
                     ANGLPLITSGMTVKVANYNPDWLGGLSNTITYKNLSLNALIDARWGGSFISFTEAITA
                     GNGILDYTAIGREDGSLLFGRDIFKNEKGVTPEGGANTKATNAEAFWNNVGGRNNPAG
                     EAFVRDATNIRLREVILGYNLPKDLVAKTFFSAARVSLVGRNLFFFMNKAEHSDPEIV
                     NSTANTDEGREAFALPTTRTFGVSLNFDF"
     gene            774..2387
                     /locus_tag="PLZ75_05200"
     CDS             774..2387
                     /locus_tag="PLZ75_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOS71827.1"
                     /translation="MKNLLIIISVLALAVFAGSCTKDFDEINTNPKAITVASLDQSSF
                     GLVAKRAIVYTSYLRNSTGMQTYHSLFGDVYANYFATTHPNFLSDRYVLVGSWLNGSF
                     NSFYQNAAPPIQFCVDFAKEKGLAVEEAIMKIWRVNAFAKYADSFGAIPYSHFGNMEK
                     AVPYDSLEELYALFFEELESAVTVLKANLSQGKSATLQAYDDVYKGDIAKWAKLGSSL
                     RLRLALHCKYVNPTLARTQAEAAVSAGVIENVADNAKVATSIDWYNAYCTITSWMEFR
                     MSADMESIMKGYLDPRVQSYFQEAKFPDPTDDPDDVVFNFEGMRNGQTKSDRQQLDFN
                     GHASDMHKSWVSLGAAGPAWRVMGADESYFLRAEGALENWNMGGTAQELYEKGIKASF
                     DEWGYSDYQNLAGEDYITSDRIPASPGTDLSDPGTVIPPVSTVPVAFMTTGGKEQQLE
                     QIITQKWLCLYPENSIEAWTVRRITKYPKLYDRLESEEPLIAATSLPTRLTFITGEYT
                     NNKAEVEKAVSKLGGPDNGTTKLWWDKKP"
     gene            2813..5827
                     /locus_tag="PLZ75_05205"
     CDS             2813..5827
                     /locus_tag="PLZ75_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS71828.1"
                     /translation="MMKKLFLFMVTLLTTTAMLFAQVRVSGTVTDENGEALPGVSIVQ
                     VGTTVGTTTDLGGNYSLNVTDANAVLRFTFVGMTTVEQPLNGRTTVNVVLHSESIGLE
                     EVVVTALGIARERKTLTYASQQVSSAEITRARDINFMSSLAGKASGLEIKKAASGAGG
                     STRTVLRGSKSLSGLSEPLYVIDGVPMVNRKGSQAGMWGGWDEGDGISQINQEDIESI
                     SVLKGSNAAVLYGSQGANGVVIINTKKGKAGTTDISINSGTIFETVLLYPELQYKYGA
                     IGNAKESWDTKPLSKYDDKFVKDFFQGGYNLVNSISISGGTDRTQAYFSYGNTSARGV
                     IPTNKYQKNNVTFKQSTKFFNDRVKVTSNVMLANEQADNRPPNGYYLNPLTGLYFFPR
                     QRDFQSYKENYKVFNADRNMYLQNWFVADHHQSNPYWIINMEPRVDQTKRAIASATVE
                     YDIAKGLKFQTRASVDYAVKENEQRHAAGSNATNVSANGTWNYKKYDDQLLYTDAIFT
                     YNTRFGEFSLDALAGGSWQKSVYGRGVSANNGTYSLFYPNEFNFQNLPQNIQVLSTLS
                     SRAVKQGLFANMQFGFKDMIFLDLSGRNDWSSTLALTGNESYFYPSIGLSALLSEMLP
                     LPDFITFSKVRASYTTVANEVPFNRINPMHSITGSGGVDRNTVAPFFDARPEMLRSLE
                     LGTEWRFFTGRFGFDLTYYNINSQDQFISLPAPVGTGYSTMYVNAGEIVNKGVELTLD
                     AQPVVTGDFMWSTTFNFARNKNIVSELWPDDPGKRVDLGSSEGYYNYIVAGGSFGDLY
                     GHQFVRNDKGRIVLDAKGAPRKTQLPELIGSLDPDFTLGWNNNLSFKQFNLSFLINGI
                     FGGNCVSQTESMLDGAGVSKRSGDDRDKGYVDVKVVTAEGTEKDKMENVELWYRTIGD
                     RNGILEAYVYDRTNIRVTQLALSYDLDVRKLNLPFKAASVSLVGQNLLFLYKKAPYDP
                     ELTMSAGLGSQSLDNFNVPATRTFGFNIKLNF"
     gene            5841..7427
                     /locus_tag="PLZ75_05210"
     CDS             5841..7427
                     /locus_tag="PLZ75_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOS71829.1"
                     /translation="MKKIISLAVLVVLLGACTKNFDEINSNPYQITQKSLEQDFNHIG
                     AYFQGLLANLQGHQVEEDLLTDSFVRHFGTPTPFVSNRNNTTYYVTWNSFWGRIYNSV
                     MSPSKQVIEIAQEGGYDVFEHWAKLLQVIGLSRLTAYHGPLIYSEYGKTTTPIYYDSE
                     KDLYNSFFKVLDDVSRVFKNNPDYKGLIKFDASYKGKISSWLKLVNSMRLRLAIRLSK
                     VDPALAKEQGEKAIADDAGLILTNADNFNISLYGGKMPIAVICFEWQDSKMSAGMEEF
                     LIGLKDPRIHKYFAPATDESLYASHPDFPYKGIASGAYLNAKDDHEKFSNVSEDWKSV
                     TSRRFFTAAEVHFALAEAALRGWAGAGDAREHYETGIKMSFADWGAGGVDAYLADATS
                     KPINYVDPIDARNNYNTRSTVTVAWDETADNEIKLEKIITQKWIDALTNANEVWCDHR
                     RTGYPKLHYVPKNDSNEDWGVIPADGFIQRMPFVEGERNSNPQGVADAASKLRGGKDV
                     IGATLYFAPPDVNTNFPPIP"
     gene            7675..8034
                     /gene="ssb"
                     /locus_tag="PLZ75_05215"
     CDS             7675..8034
                     /gene="ssb"
                     /locus_tag="PLZ75_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOS71830.1"
                     /translation="MSNSLRNRVSLIGNLGQDPLIRDLENGKKVANFTIATNQGFRNA
                     DGQKVQETTWHNIVAWNGLAETAARYLKKGREVAVEGRIVYRTYEDRNGVTKYITEIV
                     LTDLLFLRSPKSMTEEE"
     gene            complement(8183..10564)
                     /locus_tag="PLZ75_05220"
     CDS             complement(8183..10564)
                     /locus_tag="PLZ75_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOS71831.1"
                     /translation="MRYIKIVLFVALSVNTAFSQKADFRAAEKFRGDNIASRYGDLSV
                     NPVWLENSDIFWYSFKTSSGRNFYYVNAAAKSKKPLFDSRYMASELKKLTSRPYNDLD
                     LPITELKFEKKSLSRFTFRVDSIRFLYDMATRKLVIRDTVGKEKKPSWPNYSPDSTWF
                     AYARNYNLYLMKTNDRDSVEIQLTTDGERYYGYSSYRSGQDTVRDKKVRANVRWFKNE
                     KKLFIIREDVRKVKDLFVINSLAQPRPSLESYRYSMPGEEFVPQQELIIFDVETRGRI
                     NVDLKKWKDQTINVAWSSQEESNRLIVIRKDRTLKNLDVCMVDAETGDVKVLFSEKTW
                     PYFNNEYSQLAVLNEGSDIIWWSERSGWGQLYLYDIHGNIKKQITDGYFVTGRINRID
                     TLGRVLYLEAFGKEDGVHPYYNMKYKVSLDKGGMTLITGEPANHSMSMSKSCRYFVDT
                     FSTVDMVPESVLRDNNGNTLLRLEKADLSQLFQMGWKMPETIKVKAKDGVTDLYGVMY
                     KPFNFDSTRKYPVISYVYPGPQTESVPYEFTVTGGKNVALAQLGFIVVNYGHRGGSPQ
                     RSNYYHTFGYDNLRDYALADDKYGIEQLAEKYKFIDIGKVGIYGHSGGGFMSTAAILS
                     YPDFYQVAVSSAGNHDNNIYNQWWGETHDGVKEVEKKSKGGEGVKKDSLDSVKEKETT
                     GNSGSKGTVDNSQQDGKAFKSSIDRNQDLAKNLKGHLLLVHGDIDNNVHPGNTLRVAD
                     ALIKANKRFDFMILPGQRHAFGNATAYFDRLMWYYFAEHLLGDYRTNVDLTDF"
     gene            complement(10561..>10868)
                     /locus_tag="PLZ75_05225"
     CDS             complement(10561..>10868)
                     /locus_tag="PLZ75_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOS71832.1"
                     /translation="GWLITNSDPYINIPDYPPLEEIFSEIRKIKNHRIINAETTAREA
                     GSVKAVNMVMLGAASHYTGLGCSSLENGIRKLFGSKGEKITDINLTAFRAGRNIINP"
BASE COUNT         3123 a         2480 c         2461 g         2804 t
ORIGIN      
        1 ccgaaggatt ctacgttctc tcctttggaa cagactttat aagggagtat aagctcgtaa
       61 aaggtgaagc tttcggagat gtatatgcaa aaggatttca aagggatgca aatggcaatg
      121 taataataaa ccaggcaaac gggcttcccc ttattacttc cggtatgaca gtgaaggttg
      181 caaactacaa tcccgactgg ctgggaggcc tgagcaatac cataacatac aagaacctgt
      241 cacttaatgc acttattgat gccagatggg gtggttcctt tatttcattc accgaggcca
      301 taactgccgg aaacggaatt ctcgactata ccgcaatagg cagggaagac ggctccctcc
      361 ttttcggcag ggatattttc aagaatgaaa aaggtgtgac gcccgaaggc ggagcaaata
      421 cgaaagccac caatgctgaa gctttctgga ataatgttgg cggaagaaac aatccggccg
      481 gtgaggcatt cgtgagagat gccacaaata tcaggctcag ggaagtgatt ctcgggtaca
      541 atctgccgaa agatcttgtt gccaagacat tcttcagtgc agcccgggta tccctggtag
      601 gcagaaacct gttcttcttc atgaacaagg ctgagcactc cgatcccgag attgtcaaca
      661 gtactgcaaa cactgacgaa gggcgtgaag ctttcgcact gccaacaacc cggacattcg
      721 gcgtatccct taacttcgat ttttaataca gcaacatgtt aaaacaaaat aatatgaaaa
      781 acctgttaat aataatatca gttcttgcat tagctgtttt cgcaggcagt tgtacaaagg
      841 atttcgatga aataaatacc aatccaaagg ccatcaccgt ggccagcctt gaccagtcat
      901 ccttcggtct ggtggcaaaa agggcgatag tatatacttc ctacctgcgg aacagtacgg
      961 gaatgcagac ctatcactcc cttttcggag atgtatatgc aaactatttt gcaacaaccc
     1021 atccgaactt cctgtcggac aggtatgttc tggtaggcag ctggctgaac ggctcattca
     1081 attcgtttta tcagaatgcc gccccgccca ttcagttctg tgttgatttt gccaaggaaa
     1141 agggcctggc ggtcgaagaa gcaatcatga aaatatggag ggtaaatgct tttgcaaaat
     1201 acgccgattc cttcggggcc ataccttatt cacatttcgg aaatatggaa aaggccgttc
     1261 cctatgacag tctggaggaa ctatacgctc tgtttttcga ggaacttgaa agtgcagtaa
     1321 cggtgctaaa ggccaatctg tctcagggca aatcagctac cctgcaagcc tatgatgatg
     1381 tttacaaggg cgatatagca aaatgggcca aactcggaag ctccctgcgc ctcaggctgg
     1441 ctctgcattg caaatacgtc aatcccacac ttgccagaac tcaggctgaa gcagctgtct
     1501 cggcaggtgt catagaaaac gttgccgaca atgcaaaagt ggctacttca attgactggt
     1561 acaatgcata ctgtaccatt acctcatgga tggaattcag gatgagtgcc gacatggaga
     1621 gcatcatgaa gggttatctt gatccccgtg tccagagtta ctttcaggaa gccaaatttc
     1681 ctgaccccac agatgatccg gacgatgtgg tgtttaattt tgaaggaatg aggaatggcc
     1741 agaccaaatc ggacaggcag cagctggatt ttaacggtca tgcttccgac atgcacaaat
     1801 cctgggtttc tctcggtgct gccgggcccg cctggagagt aatgggtgca gacgaatcat
     1861 attttctcag ggctgaaggt gccctggaaa actggaatat gggcggaaca gctcaggaac
     1921 tctatgaaaa aggtatcaag gcatcatttg atgaatgggg atattctgat tatcagaacc
     1981 tggcagggga agattatatc acaagcgaca ggataccggc aagcccgggc acagaccttt
     2041 cagatccggg aacggttatt ccgccggtgt caacagtgcc agtggcattc atgacaaccg
     2101 gcggcaagga acagcaactg gagcagatca tcacacaaaa atggctgtgc ctgtatcctg
     2161 aaaacagtat tgaagcatgg actgtgcgga gaattacaaa atacccgaaa ctctatgaca
     2221 ggctcgagag tgaggaaccc ctgatagcgg cgacttcact gccgacaagg ctcaccttta
     2281 tcaccggaga atacacaaac aacaaggccg aagtggaaaa ggcagtaagc aagcttggcg
     2341 gacctgataa tggcactacc aaactatggt gggataaaaa accctaagga aaattgaata
     2401 attgcaaaaa ggggctgtct caaaaggaca gccttttttt gtggaaatac ctggaaagtt
     2461 tataaaataa ataaaacaca aacgaaatgt ggaaaatttt taataaataa aaccaaaata
     2521 cagaatcact gaaaaaatta taaaaagaat ctttatttaa ttgatatctt gttaattaaa
     2581 aatcccggat atttttttcc ctgacacttt aacttttgtt aacgaaattt tttttatata
     2641 ttgcgccctt tattttatgc gcccttgtag cgcataagca gagatactga acgttgcatt
     2701 gtttaaataa tatatcttac aataaaagat tttgtataaa ccagagaata acagctggct
     2761 gaagctgttt tgattcagca tatagttaat atttacccta aattaaatct ttatgatgaa
     2821 aaaattgttt ctatttatgg tgacactgct taccacgacc gcgatgctgt ttgcacaggt
     2881 aagggtaagc ggtacggtta cggatgaaaa cggagaggct cttcccggag tttcgatcgt
     2941 ccaggtcggg acaaccgttg gaacgaccac ggatctaggc gggaattact ctcttaatgt
     3001 aacggacgca aatgccgtac tccggttcac ttttgtggga atgaccactg tggaacagcc
     3061 cttgaacggc agaaccactg taaacgttgt cctgcattcg gaaagtatag gactggaaga
     3121 agtggtcgtt acggctcttg ggatcgcccg tgaaagaaaa acccttacct atgcctcaca
     3181 gcaggtttcc agtgcggaaa ttaccagggc cagggacatc aacttcatga gcagtcttgc
     3241 cggcaaggct tccggacttg aaatcaagaa agctgcctcc ggagcaggtg gttcaaccag
     3301 gacggtttta agaggaagca aatccctctc agggctgagc gaacctctat atgttattga
     3361 cggtgtgccg atggtcaaca gaaaaggaag ccaggcagga atgtggggtg gctgggatga
     3421 aggagacggt atttcccaga ttaaccagga agacattgaa agtatcagtg tcctcaaggg
     3481 ttccaatgct gcagtccttt acggaagcca gggcgcaaac ggtgttgtga taatcaatac
     3541 caagaaagga aaagccggta ccactgacat cagcataaac tccggaacca tttttgaaac
     3601 tgtgctcctg tatccggaac tccagtacaa atacggagca atcggcaatg caaaggaaag
     3661 ctgggataca aaacctcttt caaaatatga cgacaagttt gtgaaggact ttttccaggg
     3721 aggatataac cttgtgaatt ccatttcaat cagtggcggc accgacagaa cccaggccta
     3781 cttctcctac ggtaacacaa gtgccagggg tgtgattccc accaacaagt atcagaagaa
     3841 caacgttact ttcaagcagt caacgaaatt tttcaacgac agggtaaaag ttacctccaa
     3901 tgtaatgctt gccaatgaac aggctgacaa caggccgccc aacggatatt acctcaatcc
     3961 cctgacagga ctctatttct tcccgcgtca aagggatttc cagtcctaca aggaaaatta
     4021 caaggtattc aatgccgaca ggaacatgta tctgcagaac tggtttgtgg ccgaccacca
     4081 ccagtcaaac ccctactgga taatcaatat ggaacccaga gttgaccaga caaaaagggc
     4141 aattgcgagt gcaaccgttg aatatgacat agccaaggga cttaaattcc agaccagggc
     4201 cagcgttgac tacgccgtaa aggaaaatga acagcgccat gctgccggat ccaatgcaac
     4261 caacgtttca gcaaacggaa catggaacta caaaaaatat gacgaccagt tgttgtatac
     4321 cgacgctatc ttcacctaca atacccgttt cggtgaattc agcctggacg cacttgcagg
     4381 aggaagctgg caaaaatcag tttacggacg gggtgtttca gccaacaacg gaacatattc
     4441 attgttctac cccaatgaat tcaatttcca gaacctgccc cagaacattc aggtactttc
     4501 aacactgtca tccagggctg tcaagcaggg actgttcgcc aacatgcagt ttggattcaa
     4561 agacatgatc ttccttgatc tctcaggaag aaatgactgg tcctcaacac ttgcccttac
     4621 aggaaacgaa tcatatttct atccctcaat cggtcttagt gcactgctga gtgaaatgct
     4681 gcccctgccc gactttatca ccttttcaaa ggtaagagct tcatatacca ctgttgccaa
     4741 cgaggttccc ttcaacagga tcaaccccat gcactcaatt acaggaagtg gcggagttga
     4801 cagaaataca gttgctccct tctttgatgc caggcccgaa atgctgagaa gtcttgaact
     4861 gggtacggaa tggagatttt ttaccggaag attcggattt gacctgactt actataacat
     4921 caacagccag gatcagttca tttcacttcc ggctccggta ggtaccggtt attccacaat
     4981 gtatgtgaac gcaggtgaaa tcgtcaacaa gggtgttgaa ctgactcttg acgcacagcc
     5041 tgttgttacc ggcgatttca tgtggagcac cacattcaac ttcgcgagga acaagaatat
     5101 cgtatcagaa ctgtggccgg atgatccggg aaaaagagtt gacctcggca gttcagaagg
     5161 ttactacaac tatatcgtag ccggaggatc tttcggtgat ctctatggtc atcagttcgt
     5221 aagaaatgat aagggacgaa ttgttcttga tgccaaaggc gcacccagaa agacccagct
     5281 gcccgaactg ataggaagcc tggatcccga cttcacactg ggatggaata acaatctgtc
     5341 attcaaacag tttaacctca gcttcctgat caacggaata ttcggaggca actgcgtaag
     5401 ccagactgaa tcaatgctcg acggagccgg agtaagcaag agatcaggtg acgacaggga
     5461 caagggatat gttgacgtta aggtcgttac tgcagaaggc actgaaaaag acaagatgga
     5521 gaatgtagaa ctctggtaca ggaccatcgg cgacaggaac ggaatacttg aagcctacgt
     5581 atatgacagg acaaacatca gggtaaccca gcttgcgctg agctatgatc ttgatgtaag
     5641 aaagctcaac ctgccgttca aggctgcttc cgtttcactg gtaggccaga acctcctttt
     5701 cctctacaaa aaagctccct atgatcctga actgacaatg agtgcaggcc tgggaagcca
     5761 gtcacttgat aactttaacg tcccggctac caggacattc ggctttaaca ttaaacttaa
     5821 tttctaataa acagataatt atgaaaaaaa taatatcact ggcagtactg gtggttttac
     5881 ttggggcctg caccaagaac tttgatgaga tcaattcaaa tccttatcag atcactcaaa
     5941 aatcattgga acaggacttt aatcacattg gtgcttattt tcagggtttg ctggccaatc
     6001 tacagggcca ccaggttgag gaagacctgc ttaccgacag ttttgtgcgc cacttcggca
     6061 caccgactcc ttttgtttca aacagaaaca atactaccta ctatgttacc tggaactcat
     6121 tctggggccg tatttacaac agcgtcatgt caccctccaa gcaggtgata gaaatagccc
     6181 aggaaggcgg ttatgatgta tttgaacact gggcaaagct tctccaggtt ataggactgt
     6241 caagactgac cgcctatcat ggtccgctta tctattcgga atacggtaag actacaactc
     6301 cgatttatta tgactcggaa aaggatctgt ataactcttt tttcaaggtt cttgatgatg
     6361 tctcaagggt attcaagaat aatcccgatt acaaaggact gataaaattt gatgccagtt
     6421 ataagggaaa aatcagcagc tggctcaaac tggtcaattc aatgagactc aggcttgcaa
     6481 tccgcctcag caaggtagat cccgctcttg caaaagaaca gggtgaaaag gcaatcgccg
     6541 atgacgcagg gctgatcctt acaaatgctg acaatttcaa tatttcactg tatggcggga
     6601 aaatgccgat tgcagtgatc tgttttgaat ggcaggacag caagatgagt gccggtatgg
     6661 aagaattcct tatcggtttg aaggatccaa gaatccataa atatttcgca ccggcaactg
     6721 atgagagttt gtatgcttcc catcctgact ttccctacaa gggtattgcc agcggcgcat
     6781 atcttaatgc aaaggatgat catgagaagt tttcaaatgt gagcgaagac tggaaatcag
     6841 ttacctccag aagatttttc actgcagccg aggttcattt tgcacttgcc gaagcagctc
     6901 tcagaggctg ggccggtgca ggtgatgcca gggaacacta tgaaaccggt atcaaaatgt
     6961 catttgccga ctggggtgca ggtggtgttg atgcatatct tgcagatgcc acaagcaaac
     7021 ccatcaacta tgttgatccg attgatgcca ggaacaacta taatacacgt tcaactgtaa
     7081 ccgttgcatg ggatgagact gctgacaacg aaataaaact tgaaaaaatc ataacccaga
     7141 aatggatcga tgcactgacc aatgccaatg aggtatggtg cgatcacagg agaacaggtt
     7201 atcccaaact gcattatgtt cccaagaacg acagcaacga agactgggga gtcattccgg
     7261 ctgacggatt cattcagaga atgcctttcg tggaaggtga aagaaacagt aatccgcagg
     7321 gtgttgctga tgcagccagc aagctgagag gaggcaagga tgtaataggc gcaacactct
     7381 attttgcccc gccggatgtt aatacaaact ttcccccgat tccttaacag gtaaagaata
     7441 actgacagga gggtgtctca aagagacacc ctttttttta tttcccggtt tgccgactca
     7501 gccggtataa acttatataa acctgaaaaa caagaaacaa tctgatttaa tcatttgcaa
     7561 acgactataa tggactttca gacggatata aacaattaat taccggatca atttccacat
     7621 caggcctatg tttgctgaga gaatcattca tagtcaaact taaaacagga aatcatgagc
     7681 aattcattga ggaacagagt aagcctgatc ggaaacctgg gacaggaccc tctgatcagg
     7741 gatcttgaaa acgggaaaaa agtggctaat ttcactattg ccacaaatca gggattcaga
     7801 aatgccgacg gccagaaggt acaggaaacc acctggcaca atattgttgc atggaacggg
     7861 cttgccgaaa cggcggcaag atatctcaaa aaaggacggg aagtggcagt agagggccgg
     7921 attgtctaca gaacatatga ggacaggaat ggtgttacaa aatatattac cgagattgtt
     7981 ctgacggatc tcctctttct ccgttctccg aaaagcatga ctgaagaaga ataaatatgc
     8041 tgccgttccg ggctgaaagg tcaattcctg cggttccgtg aagctgttcc ccggtgcaga
     8101 aaaagcggtt ctgccgtgct gatgaggctg ttcttcaagg aaacagcctc atttgattat
     8161 tttatattca ccccgtcact tatcagaaat cagtcaggtc aacattggtc ctgtaatccc
     8221 cgagaagatg ctcggcaaaa taataccaca tcagcctgtc aaaatatgcc gtggcatttc
     8281 caaaggcatg cctctgtccg ggaagtatca tgaaatcaaa tcgcttattg gcctttataa
     8341 gagcatcagc aacccttaag gtatttcccg gatgaacatt gttgtcaata tcaccatgaa
     8401 ccagcaaaag atgacccttc agattctttg caagatcctg gttcctgtct attgatgatt
     8461 taaacgcttt cccgtcctgc tgactgttat caactgttcc tttacttccg gaattaccgg
     8521 ttgtctcctt ttccttcact gaatccagac tgtccttttt cactccttcc ccgcccttgc
     8581 tttttttctc cacctccttt accccgtcat gtgtttctcc ccaccactgg ttgtaaatat
     8641 tgttatcatg attgccggct gaagacacgg caacctgata aaagtccggg tatgaaagga
     8701 ttgcagccgt tgacatgaat cctcctccgg aatggccata gataccaact ttcccgatat
     8761 cgatgaattt atatttttcg gccagttgtt cgatgccgta tttatcatca gccagggcat
     8821 aatccctgag attgtcatac ccgaatgtgt gataataatt gcttcgctgg ggactgcctc
     8881 cgcggtggcc ataatttacg actatgaacc cgagctgggc cagcgccaca ttcttgccac
     8941 cggtaacggt gaactcatag ggaaccgatt cggtctgcgg tccgggatag acgtaggaaa
     9001 ttaccggata ctttcttgtt gagtcaaaat tgaagggttt gtacataact ccataaaggt
     9061 cagtaacccc gtctttcgcc ttcaccttaa ttgtttcggg catcttccag cccatctgaa
     9121 acaactggga caggtcggct ttctcaagtc tcagaagagt gtttccgtta ttgtcacgca
     9181 ggactgactc gggcaccata tcgaccgtgg aaaaagtgtc aacaaaatac ctgcatgatt
     9241 ttgacattga catggagtga tttgcaggct caccggtgat cagtgtcatt cctcccttat
     9301 caagtgatac cttgtatttc atgttgtaat agggatgtac tccgtcctct tttccaaagg
     9361 cctctaaata aagcactctg cccagggtat ctatccggtt tattcttcct gtaacgaaat
     9421 aaccgtcagt tatctgcttt ttgatattcc cgtgtatgtc gtacagatac agctggcccc
     9481 agccgcttct ttccgaccac catataatgt cgctgccttc attgagaacc gccagctgcg
     9541 aatattcatt gttgaaatag ggccaggtct tttcgctgaa gagaacttta acatcccctg
     9601 tttcagcatc caccatgcat acgtcgaggt ttttgagagt acggtccttt cttatgacaa
     9661 taagtctgtt gctttcttcc tgtgatgacc aggcaacatt gatggtctga tccttccatt
     9721 ttttcaggtc aacatttatc cggccccggg tttcgacatc aaaaattatc agctcctgct
     9781 gaggtacgaa ctcttctccc ggcattgaat agcggtagct ttccaggctt ggcctgggct
     9841 gagcgagcga attgattaca aaaagatcct ttaccttacg gacatcctcc cggattataa
     9901 ataatttctt ttcatttttg aaccagcgca cattggcccg tacttttttg tcccttacag
     9961 tgtcctgccc gcttctgtag ctgctgtaac cataatacct ctcaccatcg gtggtcagct
    10021 gtatttcaac actgtccctg tcgtttgttt tcatcaggta aagattatag ttcctggcat
    10081 atgcaaacca tgtactgtca ggcgagtagt tcggccatga cggctttttt tcctttccga
    10141 cagtatccct gattacaagt ttcctggttg ccatgtcgta aagaaacctg attgaatcaa
    10201 cccggaaggt gaatctggag aggctcttct tttcgaattt caattccgtt attggcagat
    10261 caagatcatt gtaagggcgg cttgtcagtt tcttaagctc ggatgccata tagcggctgt
    10321 caaaaagcgg ctttttagac tttgctgctg catttacata gtaaaaattc ctgcccgaag
    10381 aagtcttgaa tgagtaccag aaaatatctg agttctccag ccaaaccgga tttacagaaa
    10441 gatccccgta acgggatgca atattatcac ctctgaactt ttccgctgcc ctgaaatcag
    10501 ctttctggga aaaggctgta ttgactgaga gggcaacaaa caaaacaatt ttaatatatc
    10561 tcatggatta atgatattgc ggccggcccg gaaggccgta aggttaatat ctgtaatttt
    10621 ctctccctta ctgccgaaca gtttcctgat tccgttttca agactgctgc atccaaggcc
    10681 ggtatagtgt gatgcggctc ccagcattac catattcacg gcttttacag agcctgcttc
    10741 ccgggctgtt gtttcagcat taatgatccg gtgattttta attttcctga tttcggaaaa
    10801 tatttcttcc aggggaggat aatcaggtat gttgatgtag ggatcagagt ttgtaatcag
    10861 ccatcccg
//
LOCUS       DAOSPX010000072        10834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5950_length_10834_cov_3.971148, whole genome shotgun sequence.
ACCESSION   DAOSPX010000072 DAOSPX010000000
VERSION     DAOSPX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5950_length_10834_cov_3.971148"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..2103
                     /locus_tag="PLZ75_05230"
     CDS             16..2103
                     /locus_tag="PLZ75_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOS71833.1"
                     /translation="MVRQIIFAISLLITIAVFSWTAGRIISYFRLTKPGYKVQNPGKR
                     FLLMMKVAFGQTKIFRQPLTGLLHALVFWGFCIIIFGSIEMVIDGLSGSEKALKVLGP
                     LHDFLMAAGDVFAVMVAVSVIVFLIRRIFMHIPRFEGTEMKRRSHRDANISLSLILLL
                     MLSLIAMNTGYTGMKRLSGEEIAGVYPVSWFLSGLFPAGRTETFRIIHEISWWTHILL
                     IFFFANYLPWSKHFHVFLSVPNVFLSRLEPRGKLSNMDSITREVRLMMDPETAFAATS
                     PDTVPERFGVRDAEDVTWKNYFDSLSCTECGRCTSVCPANITGKMLSPRKVMMDLRAR
                     MKEKGPMMIRNGKDYNDNRSLVRDYISGEELWACTTCNACATECPVNIDQPSLIVDMR
                     RYLVLEEASAPGELKAVFSNIENNGAPWQYSAEDRLNWSNGLDFKVPVMADLHAGNEK
                     PEYLFWVGCAGAFDDRYKKVARAFATILNHLNVSYAVLGREETCTGDPARRAGNEMLF
                     QMQALQVISLLRDYGVRRIITICPHCYNTFRNEYPDLGGNFEVISYLGFIRRHIDEGR
                     LVPASGRADGRAVVTYHDPCYLGRANGLYDDPRIILQKLGNDIEEMGRNRSRALCCGA
                     GGAQMFKEAEKGKKEIFIERTEEALRTGAPVIASACPFCMTMITDGLKYKNREDEIKN
                     LDIAELVAQSLGY"
     gene            2204..3982
                     /locus_tag="PLZ75_05235"
     CDS             2204..3982
                     /locus_tag="PLZ75_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOS71834.1"
                     /translation="MEIKKNLKSGEFLVADTDAADVFIPEEFNEEQLMIAQSCRDFLA
                     MEVSPQMAELEKGNRELMKSILKKSGELGLMGVSIPEEFGGFGQSFVTQMLVAEASGA
                     GYSFSVAFMAHCGIGTLPILYYGNREQREKYVPKLASGELIGSYCLTEPGAGSDANAG
                     KTSATLSEDGKYYILNGQKMWITNAGFADIQIVFAKIDNDRVLSAFIVESSYPGVVIG
                     PDEQKMGIKGSSTCQIYYNDVKVPVENLLGKRGEGFRIALNILHMGRIKLGANVLGSA
                     KKAIEDSVRYANERKQFGVLISSFGAIKHKLAEQVIRLFTCESAVYRVSRDIDDLTDT
                     LLADCGDTGRASIEAIGHYAVEAAILKVAGSEMLDYVSDEAVQIYGGMGYSAETDVER
                     SYRDSRINRIFEGTNEINRLLVSDTAMKRAMKGDFDLFGKAAGLYAGLDNLLETMAEG
                     DDYYGEKSTYTGNFKKAILICIHGAVERYGRTLVHQQEVLNNIADMIIETYLSESLRL
                     RVQKLESLKSDLTVYRDILDVNIYDAASRIRKSAHDAIYAFAEEGQAENLVRAADTLT
                     AVRGVNVKSARRRIADKLIEDNAYKF"
     gene            4324..4689
                     /locus_tag="PLZ75_05240"
     CDS             4324..4689
                     /locus_tag="PLZ75_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71835.1"
                     /translation="MRRILGALLFVLLTGIIIISSCERHTFLVETVDPVDSVYFGAVI
                     QPLLEKKCSACHGGSKDPDLRADKSYESLVTGGYVTLPAEESRLYRKVVSSGHSAYTL
                     PEEKLKILYWIAQGAKNNK"
     gene            4716..5579
                     /locus_tag="PLZ75_05245"
     CDS             4716..5579
                     /locus_tag="PLZ75_05245"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HOS71836.1"
                     /translation="MKRTISIPVLFLFMASLFAQEEGQPAAESKPVRFIFETGLLIDN
                     QTIATPFKGGLEFEIHHRFSLIENIHNLFGIYGAANTRLGLNYGISDRLMAGIGTTKD
                     YKLQDFQWKYLVLRQTEDDKMPVSLSYYGNMVLDLREDAAFGPEASYRHIHRLSYFTQ
                     FIVARKMSEKISLQIAPSVIYFNSVPRYSDTEGYKNFNLGLSAGGRVNFFGSHSLLFE
                     YDQLLTKQDLDVQPRPNLSLGWEIGTATHCFQLFVANYSQIINQRNLVFNQNDFAGGE
                     YLVGFNITVRL"
     gene            5591..6592
                     /locus_tag="PLZ75_05250"
     CDS             5591..6592
                     /locus_tag="PLZ75_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOS71837.1"
                     /translation="MKMLFAGVLVAAAFGFYTEARALSGRADSDPGTADTLAFVSPFM
                     EDNERCFKCHGQGKYEYENELLGTTVKALMCPDRIIRREDFYISNHRSFSCTDCHSSD
                     YVVFPHPGELRMELKFNCLDCHGGDETYSIFSFEMIEEDYMESVHYKLEDEGFSCWKC
                     HDPHTYRISMRDSKNLKETILYDNNICLYCHSDYTRFQILSDREEVNFLKTHDWLPNQ
                     ASHFRSVRCIECHTQISDSILVSHLVLPKDRAVKLCNECHSKNSLLMASLYKFQSMEQ
                     RRGGFLNGIIMNESYVIGANRNAYLGTLSLAIFGVVILAIVIHVIFRIKTKANLKKG"
     gene            6594..7238
                     /locus_tag="PLZ75_05255"
     CDS             6594..7238
                     /locus_tag="PLZ75_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOS71838.1"
                     /translation="MYLYPLGIRLWHALNAILIIILIITGISMQYTDKGNLVFIIDFA
                     AAVKWHNITAIILVISYIFFLILNIVSGNAKYYRINRNNLFSDLGKQFRYYVSGMFKG
                     EKHPFPVSMESKFNPLQKLTYVLIMYAGLPLIIISGFGMMFPEITIVKLFGVSGFILT
                     DILHITMAFLVTVFLVIHIYTCTLGTKPTSLFRGMITGYHEPEEESPEEVSPEK"
     gene            7380..7799
                     /locus_tag="PLZ75_05260"
     CDS             7380..7799
                     /locus_tag="PLZ75_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOS71839.1"
                     /translation="MKKNRTILAATALFVTGIMLISLNLNAQERQAKPWPVPDEYKNM
                     KNPVAADAASIKTGGVLYTKFCASCHGKTGLGDGVKARALKDFAGDFSGEYYQNQTDG
                     EHFYKTKAGRGEMPKYEGKMSDEDIWHVVNYMRTFKK"
     gene            complement(7915..9072)
                     /locus_tag="PLZ75_05265"
     CDS             complement(7915..9072)
                     /locus_tag="PLZ75_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71840.1"
                     /translation="MAGLILASCEGPMGPTGADGKDGADGKDINETCKSCHNSNVVLA
                     KSLEYGYSQHFRGTAHDHATMAGCIPCHTHKGFLDVISKNTPATITPNPSNPSRYVNN
                     YAASASALSFPGSINCFTCHSSLHTGYSATEFFPLSTTAAVPMTMWGGAKTINFQKSS
                     GNLCAKCHQPMPVTSSNGALIDYARLTTEPAAKYDMSPVSYRTGVHYGTHGAMAAGVG
                     GIEFGSGYSNSEHVTKASCTSCHMASPTGQAGGHSFNPAGNYNGCNTTNCHSGMSATN
                     PLLTETRNYVSTKLKELASKINAIGNGHDILQKDPSDGNYHGYIDIYDAGANPTGYWN
                     NPGQLSQPFPELTNAQFGAIINYQLIYRDASMGVHNYPYIKKLLDNTIAVF"
     gene            9317..10012
                     /locus_tag="PLZ75_05270"
     CDS             9317..10012
                     /locus_tag="PLZ75_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOS71841.1"
                     /translation="MSGNISASTCSGSLTEWKNFRTLSPGELDYVGQNRFEAVYKRGE
                     IVIKQGAPASNALFLVSGLAKTYMEGPGGKNFIISIDKPGVMLTGQDSYVNSRNNYSI
                     SALTELKACFIAIDVIRHLVKVNGDFAEGLLQDICSRNFRWYDRMINLNHKKMPGRLA
                     DTLLYFAGELYMADEFEMILSRQELGEMTNMARESVVRILGEFEEAGILKSETNRIRI
                     LDRDKLRLISERG"
     gene            10042..>10834
                     /locus_tag="PLZ75_05275"
     CDS             10042..>10834
                     /locus_tag="PLZ75_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71842.1"
                     /translation="MKYFRFLFSPVFMGSLFIIFAFAMAAATFIENDYGSAAAYGMVY
                     DTRWFELILLLLTVNLIGQLVIHRLFRKEKLPVALFHLAFVLILAGAGITRYFGQEGQ
                     IHIREGERNNTCTTNERYLTYAVTDSSGVKLLENTTEHKPSKLSSRGLREKIHVRNKE
                     YELVFAGMMSDRYVFHLFGGGKPSMVMLAAGPEGSQAAGSAEADGLHFEISYGPGTMK
                     LPFSILLNEFVLERYPGSDSPSGYRSDVTVIDENENVEMPFMIFMN"
BASE COUNT         2973 a         2299 c         2774 g         2788 t
ORIGIN      
        1 tactgatatt cgcttatggt tcgtcagatt attttcgcaa taagccttct gataacgatt
       61 gctgtctttt cctggactgc cggcaggatt atcagctatt tcaggcttac aaaacccggc
      121 tacaaggttc agaatcccgg aaaaagattt ttgctgatga tgaaggttgc cttcgggcag
      181 accaaaatct tcaggcagcc cctgacagga ttgctgcatg cactggtgtt ctggggcttc
      241 tgcataatta tcttcggaag cattgaaatg gtcattgacg ggctttcagg aagtgaaaaa
      301 gccctgaaag tcctggggcc gctgcatgat ttcctgatgg ctgcaggtga tgtttttgct
      361 gtaatggtgg cagtgtcggt aatagttttc ctgatccggc ggatctttat gcatatcccg
      421 aggtttgaag gcacggagat gaaaaggcgc tcacacaggg atgcaaatat ttccctttca
      481 ctgatactgt tgctgatgct ctctcttatt gccatgaata cgggttatac cggcatgaaa
      541 aggctgtcgg gcgaagagat tgcaggtgtt tatcccgtga gctggtttct ttccggcctt
      601 ttcccggccg gaaggacgga aacattccgg atcattcatg agatatcgtg gtggacgcat
      661 atcctgctta ttttcttttt tgcaaattac cttccatggt caaagcattt ccatgtattc
      721 ctgtcggtac cgaatgtctt cctttccagg cttgagcccc ggggcaagct gtcaaacatg
      781 gacagtatca ccagggaggt caggctgatg atggaccctg aaacggcctt tgccgcaaca
      841 tcccccgaca ctgtgccgga acgttttgga gtcagggatg ctgaggatgt tacctggaaa
      901 aattactttg attccctttc gtgtacggaa tgcggcagat gtacttccgt atgtccggca
      961 aatattaccg gcaagatgct gtcgcccaga aaggtgatga tggatctccg ggccaggatg
     1021 aaggaaaagg gtcccatgat gataagaaac gggaaggact acaacgataa cagatctctc
     1081 gtgagggatt atatcagcgg ggaggaactg tgggcatgca caacatgcaa tgcatgtgca
     1141 acggaatgtc cggtgaatat tgaccagcct tcgctgatag tggacatgcg caggtatctt
     1201 gttctggaag aagcatctgc cccgggcgaa ctgaaggcgg tattcagcaa tatcgagaat
     1261 aacggagctc cgtggcagta ttcagcggaa gacaggctta actggagcaa tggccttgat
     1321 tttaaagtgc cggtaatggc agatcttcat gcagggaatg agaagccgga ataccttttc
     1381 tgggtgggtt gtgcaggcgc gtttgacgac aggtacaaga aagttgccag ggcttttgca
     1441 actatactga atcatctgaa tgtaagctat gccgttctgg gcagggagga aacatgtacg
     1501 ggcgatcccg caaggcgtgc cggaaatgaa atgcttttcc agatgcaggc actgcaggtg
     1561 atcagcctgc tcagggatta cggcgtaaga aggataatca ccatctgccc gcattgctac
     1621 aacactttca ggaatgaata tcccgatctg ggcggaaatt ttgaggttat aagctatctc
     1681 ggattcatcc gcaggcacat tgatgaagga cggcttgttc ccgcgtccgg cagagctgac
     1741 gggagagctg tggtcaccta tcatgatccc tgctacctgg gcagggccaa tggtctgtat
     1801 gatgatccgc gcataatact tcagaagctg ggcaatgaca ttgaggagat gggccgtaac
     1861 cgaagccgtg cactgtgttg tggcgccggg ggggcacaga tgtttaagga agccgaaaaa
     1921 gggaaaaagg aaatcttcat tgaacggacg gaggaagctc tccggaccgg ggctcccgta
     1981 attgcaagcg catgcccgtt ctgtatgacc atgatcacag acggactgaa atataaaaac
     2041 agggaggatg agataaaaaa cctggacata gcggaactgg tcgcacagtc gctcggatac
     2101 tgattgtgct gcggaccggt ttcacggaat agccgggcag tcccgggggt aaactgaatt
     2161 aaatcctgca ttccggagga aatgcaacaa aaaataatca ataatggaaa tcaagaaaaa
     2221 tcttaagagc ggtgagtttc ttgtggctga tacagatgcc gcagatgtgt tcataccgga
     2281 agaattcaat gaggaacagc tgatgattgc ccagtcgtgc cgcgattttc tcgctatgga
     2341 ggtcagtcct cagatggctg agcttgagaa aggcaaccgg gaactgatga aaagcatact
     2401 caagaaatca ggagaactcg gactgatggg tgtttcgata ccggaggagt tcggaggatt
     2461 cggacagtca tttgttaccc agatgcttgt tgcagaagcc agcggggcgg gatattcatt
     2521 ctcagttgcc tttatggcgc actgcggtat cgggacactc cccatactct attacggcaa
     2581 cagggaacag cgggaaaaat atgttccgaa gcttgccagc ggtgagctta tcggatccta
     2641 ttgccttacc gaacccgggg ccggaagcga tgccaatgcc ggaaaaacat ctgccacact
     2701 gtctgaagac ggtaaatatt atatactgaa cggacagaag atgtggataa caaatgcagg
     2761 atttgccgac attcagattg tgttcgccaa gatagacaat gaccgtgttc tgagtgcttt
     2821 cattgttgaa agcagttatc ccggcgtggt tatcggaccg gatgaacaga aaatgggtat
     2881 aaagggttcg tcaacctgcc agatttatta caatgatgta aaggttccgg tggaaaacct
     2941 tctcgggaaa aggggagagg gctttcgcat agccctgaat atcctgcata tgggccgtat
     3001 aaagcttggg gcaaatgttc tcggcagtgc caagaaggcc atagaggact cggtaagata
     3061 tgcaaatgaa aggaaacagt tcggtgtttt gatctcatca ttcggggcaa tcaagcataa
     3121 actggccgaa caggtgatca ggctctttac ctgcgaatct gccgtgtacc gtgtaagcag
     3181 ggatattgat gacctgacag atacgctcct ggcagactgc ggtgatacgg gacgtgcgtc
     3241 aattgaggca ataggccatt atgcggtgga agctgcgatc ctgaaggttg caggttcgga
     3301 aatgctcgat tatgtttcgg acgaggctgt gcagatatac ggaggaatgg gttattcggc
     3361 agagacggat gttgagaggt cttaccgtga ttcccgtatc aacaggatct ttgaggggac
     3421 aaatgaaatc aacaggcttc ttgtttccga taccgcaatg aaacgggcaa tgaagggcga
     3481 tttcgacctc ttcggcaagg cagccggcct gtacgccggg cttgataacc tgctggaaac
     3541 aatggcagaa ggcgatgact attacggtga aaaatccaca tacacaggga atttcaaaaa
     3601 agccatactc atatgtattc acggtgccgt tgagcggtat ggaagaacac ttgtacatca
     3661 gcaggaagta ctgaacaata ttgccgacat gatcattgaa acctatttgt ccgaatccct
     3721 gagactcagg gttcagaaac tggaatcgct gaaaagtgac ctgacggttt acagggatat
     3781 actggatgtc aatatttatg atgccgccag caggatacgc aaatcggcac acgacgccat
     3841 atatgccttt gcagaggaag ggcaggcaga aaaccttgta agagctgccg atacactgac
     3901 cgcggtaaga ggtgtgaatg tcaaatcggc aagaagaaga atcgctgaca agctgattga
     3961 ggacaatgcc tataaattct gacagaagcc ctgtaccagc cggtaaccgt catgcagcag
     4021 ttctgaaaaa ttctgaaaca ccccgccgga cgcagttccg caccggggac tgctctgccg
     4081 gagcatgccc tgaacggaaa ggggccaggt tgcgtttagc agacctgtaa caatcagaga
     4141 aactggatta taaaagaacg tttctcccta atatcacaat ttacagtgac ctaaatcagt
     4201 ttccaacatg acaaacagca tgtagtcatg atataaaatc aggctggtat tttatcgtta
     4261 atatatactg acgggtatct ttgtgaaaaa tttaacaatc atttttaaat ggaatctgga
     4321 aatatgcgga gaattctcgg agcattgctt tttgttttgc tgacaggcat aatcattata
     4381 tcatcatgtg aacggcatac ctttctggta gaaacagttg atcccgtcga ttctgtttat
     4441 ttcggggcgg tgattcagcc tcttcttgaa aaaaaatgtt cagcctgtca tggcgggtca
     4501 aaggatcctg accttagggc agacaaatcc tacgaatctc tggtaacggg ggggtatgtc
     4561 acgcttcccg ccgaggagag caggctgtac cgcaaggttg tttccagcgg tcattctgcc
     4621 tataccctgc ccgaggaaaa actgaagata ctgtactgga tcgcccaggg agcaaagaac
     4681 aataaatagt agaaaccata atgaaacccg atcaaatgaa aagaaccata tcaattccgg
     4741 tgttgttttt gtttatggcc tccctctttg cacaggaaga gggtcagcct gccgcggaat
     4801 ccaaacccgt aagatttatt tttgaaacag gcctgcttat agataaccag actattgcca
     4861 ctcccttcaa gggggggctt gaatttgaaa ttcatcacag gttcagcctt atagagaaca
     4921 ttcataatct gttcggcatt tatggtgccg caaatacaag gctggggctc aattatggaa
     4981 tttctgacag gctgatggcc ggaattggta ccaccaagga ttacaaactg caggatttcc
     5041 agtggaaata cctggtactg cgtcagacag aagatgacaa gatgccggta tcgctttcct
     5101 attacgggaa catggttctg gatctgcggg aagatgccgc tttcggtccg gaagcatcct
     5161 acaggcatat acacaggctt tcatatttta cccagttcat tgttgcccgc aaaatgagcg
     5221 aaaagatttc gctgcagata gcaccttccg tcatttattt caattccgtc ccgagataca
     5281 gcgataccga aggctataaa aatttcaacc tgggactgtc agccggcgga agggtgaatt
     5341 ttttcggaag ccattcactt ctttttgaat atgatcagct tctgacaaag caggaccttg
     5401 atgttcagcc caggcccaat ctttcactgg gctgggaaat cggaacggca acccattgct
     5461 tccagttgtt tgtggcgaat tattcccaga taatcaatca gagaaatctc gtgttcaacc
     5521 agaatgattt tgcaggcggt gaatatcttg tcggattcaa cattacagta agactttaat
     5581 acttctcagg atgaaaatgc tctttgccgg tgtgttagtt gctgcagcct tcggttttta
     5641 tactgaagcc cgggccctgt ccggaagagc tgattcagac cctggcaccg cggatacact
     5701 cgcttttgta tctcctttca tggaagataa tgaaagatgt ttcaaatgcc acggtcaggg
     5761 taagtacgaa tatgaaaatg aactgcttgg cacgacggtg aaagccctga tgtgtccgga
     5821 ccggataatc aggcgtgagg atttctatat ttccaatcac aggagtttca gctgtaccga
     5881 ctgtcattcc agtgattatg ttgttttccc tcatccggga gagttgagga tggaacttaa
     5941 gttcaactgc cttgattgcc acggcggtga cgagacctat tccattttca gttttgagat
     6001 gatagaggaa gattacatgg aaagtgtgca ctataaactt gaggatgagg gcttcagctg
     6061 ctggaaatgc catgatcccc atacatacag gatcagtatg agggacagca agaacctgaa
     6121 ggagacgatt ctttatgaca ataacatctg tctttactgc cattcagact atacccgttt
     6181 ccagatcctt tccgaccgcg aagaagtgaa tttcctgaaa actcatgact ggcttccgaa
     6241 tcaggcttct catttcagaa gcgtaagatg tatagaatgc catacacaga taagcgacag
     6301 catactcgtt tcacacctgg ttcttcccaa ggacagggca gtaaagctgt gcaatgaatg
     6361 ccactcgaaa aactcattgc tgatggcatc gctttacaag ttccagtcaa tggagcagcg
     6421 aaggggcgga ttcctcaacg gcataataat gaatgaatcc tatgttatag gcgcaaaccg
     6481 caatgcctat ctcggaactc tcagtctggc catcttcggt gttgtgattc tggctattgt
     6541 tatacacgtg atattcagga taaaaaccaa agcaaactta aaaaagggct aaaatgtact
     6601 tgtatccgct ggggataaga ttatggcatg ccttgaacgc aattctgata atcattctga
     6661 ttattacagg catcagcatg cagtataccg acaagggcaa cctggtgttc atcattgatt
     6721 ttgcagctgc cgtaaaatgg cataatataa ccgccataat acttgtaata agctatattt
     6781 tctttctgat actgaacatt gtttcaggaa atgcaaagta ctacaggata aaccggaata
     6841 atttgttttc agatctcgga aaacagttca gatactatgt ttcaggaatg ttcaaagggg
     6901 aaaagcatcc ctttcccgtc agcatggaaa gcaaattcaa tccccttcag aaactcactt
     6961 acgttctgat aatgtatgcg gggcttcctc ttataataat ctcgggtttc ggcatgatgt
     7021 tcccggaaat aaccatagtg aaacttttcg gtgtaagcgg attcatactg actgatattt
     7081 tacatataac aatggcattc ctggtgacgg ttttcctggt catacacatt tatacctgca
     7141 cccttgggac aaagccgacc tcgcttttca gggggatgat tacaggatac catgaaccgg
     7201 aagaagaatc accggaagaa gtatcaccgg aaaagtaaac gtaaggggaa ttattggaag
     7261 aaagattttc ggataaggaa gcagaagagg gaataaaagg cagagggatc agcggagggt
     7321 gaacagatag aaaaaattgc atgaaatttg tattattaac ggaaaattaa atttatacca
     7381 tgaaaaaaaa cagaaccatt ttagcagcaa cagcactttt tgttacgggc ataatgctca
     7441 tttcgttgaa tctgaatgcc caggagaggc aggcgaagcc atggccggtg cctgacgaat
     7501 acaagaacat gaagaatccc gttgcagccg atgcagccag tataaagaca ggaggagtcc
     7561 tttacacgaa gttctgtgca tcctgtcacg gcaaaaccgg gctgggtgac ggagtaaagg
     7621 caagggccct gaaggatttt gcaggggatt tctccggtga atactatcag aatcagaccg
     7681 acggggagca tttttacaag acaaaggccg gaagaggcga gatgcccaag tatgagggga
     7741 aaatgtcgga tgaggatatc tggcatgtgg taaactacat gcggaccttc aagaaataaa
     7801 cggatagttc atccggatcg ctgataaaag agttattgat aaaaaacgag gctgtctcag
     7861 taatgggaca gcctttcgtc atcttgttta ctatatggtg acgaccaggt ttcgtcagaa
     7921 tacagcgatc gtattatcca gcagtttctt tatatagggg taattatgta cacccatgct
     7981 tgcatccctg tagatcagct ggtagttgat tatagcaccg aactgtgcgt ttgtgagttc
     8041 cgggaaaggc tgtgacagct gtccgggatt attccagtag cctgtcggat tcgcccctgc
     8101 atcataaata tctatataac cgtgatagtt accgtctgag ggatctttct gcagaatatc
     8161 atgtccgtta ccgattgcat tgattttgga tgcaagttcc ttcagttttg tggatacata
     8221 attcctggtt tctgtaagta agggatttgt ggcactcatt ccggaatggc agtttgttgt
     8281 attgcagccg ttgtaattgc ctgccggatt gaaagagtga cctccggcct ggcctgtggg
     8341 tgaagccatg tggcatgatg tgcaggaagc ctttgtcaca tgttcagagt ttgagtatcc
     8401 tgatccgaat tcgattcccc cgactcctgc agccatggcg ccatgtgttc cgtaatgcac
     8461 gcctgtcctg tagctgacag gtgacatatc atattttgcg gccggttccg tggtcaggcg
     8521 ggcgtaatcg atgagggcac cgtttgaaga tgttacaggc atgggctggt ggcatttagc
     8581 acaaagattt cccgaactct tctggaagtt gatcgttttt gccccgcccc acatcgtcat
     8641 aggcaccgct gccgttgttg aaaggggaaa aaactcggtg gcactgtacc ctgtgtgaag
     8701 ggaactgtga caggtgaagc agtttatgct tccgggaaat gataaggcgg atgcacttgc
     8761 cgcatagttg ttcacgtatc tcgacggatt cgagggattc ggggtaatcg tggcaggagt
     8821 gtttttgctg atcacatcca gaaaaccctt gtgggtatgg cagggaatac agcctgccat
     8881 ggtggcatga tcatgagcag ttcccctgaa atgctgtgaa tatccgtatt caaggctttt
     8941 tgccagtacc acattgctgt tgtgacatga cttgcaggtt tcattgatat cctttccgtc
     9001 ggccccgtcc ttgccatcag cacccgtagg ccccatcggt ccttcacatg aggccagaat
     9061 caaccccgcc atgcttatta aaaataaaaa acgcttcgcc ttgttcataa tcctttcctt
     9121 tatatggttt ttaatgcgga tgcaatctac gaaaacacag tgttatcttt tcagggcttc
     9181 ctatgctatt tacaatagag atcaatgata gattatatcc gcaccgctga tttatgtcaa
     9241 taaatatatt gatatttgcc ggaataacga actgattccg gtatgtaatt attccggtac
     9301 tgttaatttg atcagaatga gcggaaacat ttcagcaagc acatgcagcg gcagcctgac
     9361 ggaatggaag aacttcagga ccctcagtcc cggtgaactt gattatgtcg gtcagaacag
     9421 gtttgaagca gtctacaagc gcggggaaat agtgatcaag cagggtgctc ccgcttccaa
     9481 tgccctgttt cttgtttcag ggcttgccaa gacatacatg gaaggcccgg gcgggaaaaa
     9541 cttcataata agcattgaca agcccggggt aatgctcacc ggccaggatt cctacgtgaa
     9601 ttccaggaat aattactcca tttcggcact tactgaactg aaagcctgtt ttatcgccat
     9661 tgatgtgatc cggcatctgg taaaggttaa cggtgacttt gccgaaggac tgcttcagga
     9721 tatatgttca agaaatttca ggtggtatga caggatgata aacctgaacc ataagaaaat
     9781 gcccggaaga cttgctgata ctcttttata ttttgccggc gaactataca tggccgacga
     9841 gttcgaaatg atactttcgc ggcaggaact aggagagatg acgaatatgg caagggagag
     9901 tgttgtaagg atactggggg aatttgaaga ggccggcatt ttgaaatctg aaacgaaccg
     9961 gataaggata cttgacaggg acaagctgag gcttatttca gaaagaggct gaacatcaaa
    10021 gaaagacgta atatcacagt tatgaaatat ttcaggtttc tattctcccc ggtcttcatg
    10081 gggtcattgt ttataatttt cgcattcgcc atggctgcgg ccactttcat tgaaaatgac
    10141 tatggctccg cggctgcata cggaatggtg tatgatacca ggtggtttga actgatactg
    10201 ctccttctga ccgttaatct catcggtcag ctggtgatac acaggctgtt cagaaaagag
    10261 aagcttcccg tggcactgtt tcacctggca tttgtcctga tacttgccgg agcaggcatt
    10321 acccgttatt tcggacagga agggcagatt catatccgtg aaggggaaag gaacaatacc
    10381 tgtactacaa atgaaagata tctgacttat gccgtcacgg attcttcagg agtcaaactt
    10441 ctggaaaaca ccactgaaca caagccttcg aaattgtcct ccaggggctt aagagagaaa
    10501 atacatgtca ggaacaagga atatgagctg gtttttgcag ggatgatgtc tgacaggtat
    10561 gttttccatc tcttcggcgg agggaagccc tcaatggtca tgcttgcagc gggaccggag
    10621 ggatcgcagg ctgcaggatc ggcggaggcg gatggcctgc attttgaaat aagctacggc
    10681 ccgggaacga tgaagctgcc tttcagtatt ctgctgaatg agtttgtgct cgagagatat
    10741 cccggatccg acagtccttc aggttaccgg agtgatgtaa ctgtgattga tgaaaacgaa
    10801 aatgttgaaa tgccctttat gatcttcatg aaca
//
LOCUS       DAOSPX010000073        10763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_5994_length_10763_cov_4.647366, whole genome shotgun sequence.
ACCESSION   DAOSPX010000073 DAOSPX010000000
VERSION     DAOSPX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_10763_cov_4.647366"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLZ75_05280"
     CDS             complement(<1..754)
                     /locus_tag="PLZ75_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS71843.1"
                     /translation="MKISQFLIFFTIVFIIYFIGNLYIFLKGYSFVPRTGRLTYTVVF
                     ISLATTFIVAKFLETRHSTVFSDILNIAGGFWIAFLFYGFIFFLLSDILFILFRITGI
                     LHPENIPLYRRWAFILILAGSSLLIMGGFINAVIPVVKKYEISLPKKTEGVSTMRIAA
                     VSDIHLGSIIRKRSIRKLSSILEEIKPDAVFLLGDIIDGELGPVLRGDLLKYFTGPDT
                     RDGLYAITGNHEYIGGADRTIPYIESRGIRILR"
     gene            complement(751..1776)
                     /locus_tag="PLZ75_05285"
     CDS             complement(751..1776)
                     /locus_tag="PLZ75_05285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS71844.1"
                     /translation="MIKTAVVILNWNGLKYLKMFLPGVVRHTAGTGTEIFVADNGSTD
                     GSQDWITSEVSNVRLIRLEKNHGFAEGYNLALAQIDSQYYVLLNSDIEVTPRWLDPLV
                     KYMDSSPDTASCQPKILSYHNRDYFEYAGAAGGFIDKYGYPLCRGRVLFRIEKDRGQY
                     DSRTDIFWSTGACMIVRSSAWKECGGFDPDFFAHMEEIDLCWRFHKAGYRVSFVPDSA
                     IYHVGGGALPYESPFKTYLNFRNSLFLLYKNLPDREFRRTMFKRKILDGIAAVFFLLK
                     GQFRSSLAVLRAHVHYYRNIGKLRQKRKMARACGQKEAHGLILNKCLVFEFYILKKRT
                     FDILTSR"
     gene            complement(1787..2650)
                     /locus_tag="PLZ75_05290"
     CDS             complement(1787..2650)
                     /locus_tag="PLZ75_05290"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOS71845.1"
                     /translation="MEAAGFYIFYGISWAISLLPLKILYLFSDFLFLLAFYFPGYRRK
                     VVITNLRNAFPEKSERELAAISKKFYRHFCDLVVEILKLIHISNEELAERMHYENPGL
                     LDRLFAEGRDVVMVYSHYCNWEWTNILPAFTGHKCVPVYRALNNKHFDRFMLGIRSRN
                     KATPVAMSAIIREILTNRKNSVKAVYGLIADQTPPRAEIKYRTRFLNQDTPVYLGAEK
                     IALKYDMAVVFMNIRKIRRGYYSYSTEMLFEHTSGLEEYSVTDSHVRRLEEIIREKPE
                     YWLWTHRRWKY"
     gene            complement(2661..3458)
                     /locus_tag="PLZ75_05295"
     CDS             complement(2661..3458)
                     /locus_tag="PLZ75_05295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOS71846.1"
                     /translation="MKDLKSIVSEVAELARETGKFIRRESAGFDISRTEKKGLHDLVS
                     YVDRKSEEMLVEKLSLILPEAGFITEEGTSSKKCGRYCWVIDPLDGTTNFVHGLQPYA
                     ISIGLLEDEEPVAGVVYEAVGSETFTAWKNGGAWLNDRAIHVSEIKSFSDSLIATGFP
                     YNDFSRLNEFLESLKHFSKTTHGIRRLGSAAIDMAYVACGRFETFYEYGLNPWDIAAA
                     TIIVREAGGKVSDFRGNEKNLSGSDVIASNNLLYSEFLETVAKFMNS"
     gene            complement(3458..4768)
                     /gene="mtaB"
                     /locus_tag="PLZ75_05300"
     CDS             complement(3458..4768)
                     /gene="mtaB"
                     /locus_tag="PLZ75_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOS71847.1"
                     /translation="MNDSRKKKIAFHTLGCKLNFAETSTLARSFPPERFERVADGQKA
                     DIYIINTCTVTGIADRKCRQAVKRIISRSPDAFIAVTGCYAQLRPEELASIPGVDLVL
                     GTNERFNIAGFLEDTSKRRGAEIHSCELSDSDGFMHSYSAGDRTRSFLKVQDGCDYNC
                     SYCTIPMARGKSRNPGIQTLLDEARAIAAGGVKEIVLTGVNTGDFGRSTGESLAELLK
                     ELVRVEGIERYRISSVEPNLLTDGLIEICSAEPRIMPHFHIPLQSGCDRILALMRRRY
                     RKDLFAGRVRHISEAIPLAGIGADVIVGFPGENEADFAETYSFLENLPLSYLHVFPFS
                     ERPGTAAEKLPGKVPHELKSLRSKKLLELSQKKALEFRERNRGKNAEVLFEHVRNKGM
                     IYGFTGNYIRVEHPWEAALAGTVRKVKILGMSASGNMNIELNRS"
     gene            complement(4785..5468)
                     /locus_tag="PLZ75_05305"
     CDS             complement(4785..5468)
                     /locus_tag="PLZ75_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS71848.1"
                     /translation="MKIKHVKTILVLLTVAAIVSFSSCGRTKTKKDGGETASATEKVM
                     LFNGKDLSNWTFFLRDPSADASEVFTIKNGVIHISGDPFGYMRTGEVYGNYALHVEWR
                     YPTEATNSGVFIHVQQPDTIWPKCFEVQLKAGNAGDLLCMGGSDMNERTDKSTIIVAK
                     LAESAEKPVGEWNKMEITCISDNIDVTLNDVLMNRATGTSLKEGHICLQSEGRAIEFR
                     NVWLTRLKE"
     gene            complement(5498..6832)
                     /locus_tag="PLZ75_05310"
     CDS             complement(5498..6832)
                     /locus_tag="PLZ75_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOS71849.1"
                     /translation="MAKKHNKKIFIIDTNVLLHDYKCIYNFEENDVIIPIVVLEELDR
                     FKKGNDQINFHAREFTRELDKLSGDLLLTANIPLGENLGNLHIETGKEFSEKVSQSFP
                     DRTPDHRILAIADYVCNANKNKTVVLITKDINLRMKAKSLGIMAQDYRTDKVANIDDL
                     YKGISVVEGIDQVLINKLYDNPAGVSVSEFNLDQEQKGHKFFIMKNNGASALAHYNPA
                     SKLLNRVVKQTTYGIDPRNAEQTFALDVLTNPEIQLVSLTGKAGTGKTLLALAAALAQ
                     HKRYKQIFLARPIVPLANRDLGFLPGDIKDKMDPYMQPLYDNLTVIKHRFSHQSPEFL
                     RINDMMKEEKLVITPLAYIRGRSLSSIFFIVDEAQNLTPHEIKTIITRAGEGTKMVFT
                     GDIEQIDSPYLDTGSNGLSYLSDKMKDQDIFAHVNLVKGERSYLAELASKLL"
     gene            7033..8412
                     /locus_tag="PLZ75_05315"
     CDS             7033..8412
                     /locus_tag="PLZ75_05315"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOS71850.1"
                     /translation="MNYSETLDFLYSRLPAYHRIGRAAYRNNLDNTLALDAYFGHPHR
                     NYLSVHIAGTNGKGSVSHMLASILMEAGYKTGLYTSPHLKDFRERIRINGKMIPKGRV
                     VSFVRNHRGIIETVQPSFFELTAAMAFDYFSREGVDVSVIETGMGGRLDSTNIITPVL
                     SVITNIGHDHADLLGDTPEKIAAEKAGIIKTNIPVVIGETQSETMEIFCESARASHSR
                     IFFADRHYRCTATVDSADPELRYYTVERPAGGEAFTGEDPCNRGNALTSVNPPTSGNP
                     SAAVKRVNVAVPLVGDYQVRNIQTVFQACECLSDSLAIDEKAVVGGLRKVVSNTGFSG
                     RWQILQKRPLVIADTGHNREGIEYVVRQIGKMNAERIHFVLGFTGDKDLSLVLPLFPV
                     NAFYYFTRASVSRALDEKLLQKEALRYGLRGESYGTVADAWKAALDNAAADDIIFIGG
                     STFVVADVL"
     gene            8528..10324
                     /locus_tag="PLZ75_05320"
     CDS             8528..10324
                     /locus_tag="PLZ75_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOS71851.1"
                     /translation="MKKIIVSLSFILLVFSLEAQPDSKIVIIHSNDLHSHLAGFAPEL
                     DYSPLVTGNDGTVGGFARIASIIRSEKEKNEGITLVLDAGDFLMGTLFQGVEIKTGFQ
                     LRLMKTMGYDVLCIGNHEFDYGPARLADIISSSAGAGEIPALLLGNAVFDPADPEDDG
                     LEKLFSDNVLRRTMIINRGGIRFGFFSLLGKVAVGNAAYAPPVKFSKQIASARQMVKE
                     LKDEGCDVIICLSHSGVSRDKKGRWAGEDVELARKVKGIDVIVSGHTHTALEQPVMVG
                     GVPVVQTGEYGMNMGKLTLEYKGGKLKVSDYSLIPVNDNIAGDRHIHDLIELQKERVN
                     EDILKPAGLSYDKPVVETDFLLECDEGGDIRNSNLGPLVADAIHSYVNNHVRTGTDIS
                     MVAVGVIRDRIVPGYQSAPDIFRIMSMGTGNDAIPGYPLSRVYLTGKELKSVLEILQL
                     AYRSTPENYCYYSGLRVMHDPGRMMFRKIKRIELVKADGNVKQVDFSRKNKELFSVTA
                     NSYMLEFVGIIKKMSFGLLNVVPKDINGNPLTDMKKAILDFDENTPGIQEGREWLALV
                     EYLSSMEDTNFNNIPDIDSKYREAIQTFIPVK"
     gene            complement(10337..>10763)
                     /locus_tag="PLZ75_05325"
     CDS             complement(10337..>10763)
                     /locus_tag="PLZ75_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOS71852.1"
                     /translation="VADINVDMIGRVKGIADTTSDYPMTGLNEVFVITGNQSRELSGI
                     ARDVDERIPLDFDYSLSGRDHPLQLFARSDHYNFVRKDIPVLFFTTGLHTDYHTPGDV
                     IGKINFGKMERITEALFQIGYTVANNKKRLKVNNPFSKW"
BASE COUNT         2659 a         2438 c         2577 g         3089 t
ORIGIN      
        1 ccctgagtat tcttataccc ctgctttcga tgtacgggat ggttctgtca gctcccccta
       61 tatattcatg gttgcctgtt atggcataca gtccgtctct cgtgtcaggt ccggtgaagt
      121 atttcaggag atcgcccctg agaaccggac caagttcccc gtctataata tcgccaagca
      181 ggaataccgc atccggtttg atttcctcaa gtattgaaga aagttttcta atgctccgct
      241 tgcggataat actcccgaga tgaatgtcgg aaactgcggc aattctcatg gtgctgacac
      301 cttctgtctt cttcggaagg gatatttcat atttttttac caccggaatg actgcattga
      361 tgaaaccgcc catgatcaga agagatgagc ctgcaaggat cagtatgaag gcccacctgc
      421 ggtaaagcgg gatgttttcc ggatgaagga tgccggtgat cctgaacagg atgaacagta
      481 tatctgacag aaggaaaaaa atgaagccat agaaaaggaa ggcaatccag aatccccctg
      541 caatgttcag tatgtctgag aaaacagttg aatgcctggt ttccaggaat ttggctacga
      601 taaaggttgt ggcgagtgaa atgaacacta ctgtgtaggt cagtctgccc gtccgcggca
      661 caaaactgta acccttaaga aagatataca ggtttccaat gaaatatatg ataaaaacga
      721 ttgtgaaaaa tatgaggaac tgcgatattt tcatcgtgat gtgagtatgt caaatgttct
      781 tttcttcagg atataaaact cgaaaaccag gcatttgttc agtatcagtc cgtgagcttc
      841 cttttgcccg caggccctgg ccatttttct tttctgcctt agtttcccga tattcctgta
      901 atagtgcaca tgggccctta aaacggccaa ggagcttctg aactgtccct tcaggagaaa
      961 aaatactgcc gctatgccat cgagaatttt tcttttgaac attgttcttc tgaattccct
     1021 gtcgggcagg tttttataca gaaggaacag gctgttccgg aaattcaggt atgtcttgaa
     1081 aggagattca tacggaagtg cacctccgcc gacgtgatag atggcagaat caggcacgaa
     1141 tgaaacacgg tagccggctt tgtggaagcg ccagcaaaga tctatttctt ccatatgggc
     1201 gaagaaatcg ggatcgaatc ctccgcattc cttccatgct gaggatctga cgatcataca
     1261 ggcaccggtt gaccagaata tatctgtccg gctgtcatac tgccccctgt ctttttcaat
     1321 tctgaaaagg acacgtcccc tgcagagggg atagccgtat ttgtcaataa acccgcctgc
     1381 agctccggca tattcaaaat aatccctgtt gtggtatgac aggattttag gctggcatga
     1441 agccgtatcg ggactggaat ccatatattt caccaggggg tcgagccacc ggggagtaac
     1501 ttcgatatcc gaattcagga gtacgtaata ttgtgaatct atctgtgcca gtgccagatt
     1561 atagccttcc gcgaagccgt gattcttttc aagccggata agtctgacat tgcttacttc
     1621 cgaggtgatc cagtcctgtg atccgtcagt tgaaccattg tctgccacga atatttccgt
     1681 tccggtaccg gcggtatgcc ttaccactcc cggaagaaac attttcagat atttcagtcc
     1741 gttccagttc aatatgacaa ctgcagtttt gatcattgtc agtgtcttaa tatttccatc
     1801 tcctgtgagt ccataaccag tattcgggtt tttccctgat tatttcttca agtctcctga
     1861 catggctgtc ggttacggag tattcctcca gccctgatgt atgttcgaac agcatttccg
     1921 ttgaatagga ataatagcct ctccgtattt tccggatatt catgaacacc acggccatat
     1981 cgtatttaag ggctattttt tctgctccga gatataccgg tgtgtcctgg ttcaggaacc
     2041 tggtcctgta ttttatctcc gccctggggg gagtctggtc ggctataaga ccataaactg
     2101 ccttaacact gtttttcctg ttagtgagaa tttcccgtat tatagcggac attgcaacag
     2161 gagtggcctt gtttctcgat ctgattccga gcatgaacct gtcgaagtgc ttattgttca
     2221 gggccctgta cacagggacg catttatgtc cggtgaatgc cgggaggatg ttcgtccatt
     2281 cccagttgca gtagtggctg tacaccatga ctacatcacg tccttcggca aagagtctgt
     2341 caagcagtcc gggattttca taatgcatcc gttcagccag ctcctcattg cttatatgga
     2401 tgagtttcag tatttccact accagatcgc agaaatggcg gtagaacttt ttgctgatgg
     2461 ctgccagctc cctttcgctt ttttccggaa atgcatttct cagatttgtt attacaactt
     2521 ttcgccggta tcccgggaaa taaaatgcca gaaggaagag gaagtcggaa aacaggtata
     2581 atatcttcag tggcagcagg cttattgccc agctgatccc gtagaagata tagaatcctg
     2641 cagcctccat agatccggga ttagctattc ataaatttag cgacggtttc gagaaattca
     2701 gaataaagaa gattgtttga agcgattacg tcggaacctg aaagattttt ttcattgcct
     2761 ctgaagtcgc ttacttttcc tcccgcctcc ctcacgatta ttgttgctgc ggctatatcc
     2821 cagggattaa ggccgtattc atagaatgtt tcgaatcttc cgcaggcgac ataggccatg
     2881 tcaattgcag ccgaaccgag tcttctgatc ccgtgggtgg tctttgaaaa atgtttcagg
     2941 ctttccagga actcattcag cctgctgaaa tcgttgtacg ggaatcccgt ggcaatgaga
     3001 ctgtcggaga agctttttat ttcagacaca tgaatggccc tgtcattgag ccaggctccc
     3061 ccgtttttcc atgcggtgaa ggtttccgat ccgactgctt cataaacgac gccggcaacc
     3121 ggttcttcat cttccaggag tccgatgctt attgcatagg gctgaaggcc atggacaaag
     3181 ttggtggttc cgtcaagagg atcaattacc cagcaatatc ttccgcattt ttttgatgat
     3241 gttccctctt ccgtaatgaa gccggcttcc ggaaggatca gactcagttt ctccaccagc
     3301 atttcttccg acttcctgtc gacatagctt acaaggtcgt gaagtccctt tttttcagta
     3361 cggctgatat cgaatcccgc cgattcacgc cggatgaatt ttcctgtttc gcgtgcaagt
     3421 tctgccactt cactcactat ggattttaaa tctttcatta tgatctgttt aattcaatat
     3481 tcatatttcc ggatgcagac atgccgagta tttttacttt cctgaccgtt ccggcaaggg
     3541 ctgcctccca gggatgttca accctgatgt agtttccggt gaaaccatat atcattcctt
     3601 tgtttctgac atgttcgaaa agtacttccg cgtttttccc cctgttccgt tccctgaact
     3661 ccagtgcttt tttctgtgac agttccagga gttttttact tctcagactc ttcagctcgt
     3721 gcggaacttt tcccggaagc ttttcagcgg cagttcccgg cctttccgaa aagggaaaga
     3781 catgaaggta tgagaggggc agattctcca gaaaggaata agtctctgca aaatccgctt
     3841 cattctctcc ggggaagccc actataacat cggccccgat accggccagc gggattgctt
     3901 cgcttatgtg ccttaccctt ccggcaaaca ggtcctttct gtatctcctt ctcatgaggg
     3961 caagtattct gtcacatccg ctctgcagcg gtatgtggaa gtggggcatg atccgtggtt
     4021 ctgccgagca tatttcaata agcccgtccg taagcaggtt gggttcaacc gaggatatcc
     4081 tgtagcgctc tatcccttcc accctgacca gttccttcag aagttccgca agtgattccc
     4141 ctgtggatct gccgaaatcc cccgtgttga caccggtcag gacgatttcc ttcacgcctc
     4201 cggcagctat tgccctggcc tcgtcaagca gtgtctgtat tccgggattc cggctttttc
     4261 cccttgccat gggaatggtg cagtaggaac agttataatc acatccgtcc tgcactttca
     4321 gaaaggatct tgtgcggtcg ccggcggaat aggaatgcat gaaaccgtca ctgtcagaaa
     4381 gctcacagga atgaatctcg gccccgcgcc ttttgcttgt atcctcaaga aatccagcaa
     4441 tattgaatct ctcgttggtt cccagcacga gatcgactcc cggtatggaa gcaagttctt
     4501 caggcctgag ctgtgcatag catcctgtaa cagcaataaa tgcgtcaggc gaccggctga
     4561 tgattctttt tacggcctgc cggcatttcc tgtcagcaat accggttacc gtacaggtgt
     4621 tgataatata tatatcagcc ttctgtccgt cggcaaccct ttcaaatctt tccggcggga
     4681 atgatcttgc aagggttgat gtttcagcga agtttaactt gcagccaaga gtatgaaatg
     4741 ctattttttt ctttctgctg tcgttcatgc aatattcctt caggctattc tttcagcctt
     4801 gtaagccata catttctgaa ttcaatggcc ctgccctcgc tctgcaggca tatgtgaccc
     4861 tccttcaggc tcgtaccggt cgccctgttc atcaggacat cattaagcgt aacatcaata
     4921 ttgtcagaaa tacaggttat ttccatcttg ttccattcac ccacgggctt ttctgctgat
     4981 tcagccagtt tggccacgat aatggttgat ttgtcggttc tttcattcat gtctgagccg
     5041 cccatgcaca ggagatctcc ggcattaccg gccttcagct ggacttcaaa gcatttcggc
     5101 cagattgtgt caggctgctg aacatggatg aaaacaccgc tgttcgtggc ttccgttggg
     5161 tatctccatt caacatgaag ggcataattg ccgtaaactt cccctgttct catatacccg
     5221 aaaggatcac cgctgatgtg gatcacgccg ttttttatcg tgaacacttc cgaggcatct
     5281 gccgaaggat ctctcaggaa gaaggtccag ttgctgaggt ctttgccatt gaagagcatt
     5341 actttctcag tggctgatgc tgtttcgccc ccgtcctttt ttgtttttgt acggccgcat
     5401 gaactgaatg atacaattgc ggcaacagta aggagaacca ggatagtttt tacatgtttg
     5461 attttcataa taatttgtat ttaagttatt tgaattatca gagaagtttg ctggccagtt
     5521 cagcaaggta gcttcgttct ccctttacaa ggttgacgtg tgcgaatatg tcctggtctt
     5581 tcattttatc ggagagatag ctcagaccgt ttgatccggt gtcaagatag ggagagtcaa
     5641 tctgttcgat gtcgcctgta aagaccatct tggtaccttc ccctgcacgg gttatgattg
     5701 tttttatttc gtggggggta aggttctggg cttcatccac gatgaagaat atgcttgaaa
     5761 gacttcttcc cctgatgtat gcaagggggg taatgaccag tttttcctct ttcatcatgt
     5821 catttatcct gaggaattcc ggactctgat gactgaacct gtgtttgatg actgtcaggt
     5881 tgtcatacag gggctgcata tacggatcca tcttgtcctt gatatcgccg ggaaggaagc
     5941 caaggtcgcg gtttgcaagc ggaactatcg gccgggccag gaagatctgt ttgtatctct
     6001 tatgctgggc aagtgcagct gccagggcaa ggagggtttt tcccgttcct gcctttcccg
     6061 tgagcgacac gagctggatt tccggatttg tcagaacatc aagggcaaag gtctgttcag
     6121 cattacgggg atcaatacca taggtggttt gcttgacaac cctgttcagg agtttgctgg
     6181 caggattgta atgggccagg gcgctggctc cgttattctt catgatgaag aacttatggc
     6241 ctttctgttc ctggtcgagg ttgaattccg acacgcttac tccggcggga ttgtcgtaga
     6301 gcttgtttat cagcacctga tcaatgcctt ccacgacgct gatacccttg tacaggtcat
     6361 ctatgttcgc caccttgtcg gtcctgtaat cctgtgccat gatgcccagc gacttggcct
     6421 tcatccggag gtttatgtct tttgtgataa gaaccaccgt tttgttttta ttggcattgc
     6481 agacataatc agcaatggca agtatccggt gatcgggggt tctgtcagga aatgactggc
     6541 tgactttttc cgagaattcc ttgccggttt cgatatgcag attccccagg ttctcaccaa
     6601 gaggaatatt ggcagtcaga agcaggtcgc cggataattt gtcaagctcc ctggtaaatt
     6661 cccttgcatg aaaattaatc tggtcatttc ccttcttgaa cctgtccagc tcttccagta
     6721 ctacaatagg tataatcaca tcattttctt caaaattgta aatgcacttg taatcatgta
     6781 aaagaacatt cgtgtcgatt atgaagattt ttttgttgtg tttttttgcc atgtttaaat
     6841 atctgataat caaaatggta ttttgcaaat atccttccgg gtgccggaaa tgcaaaaata
     6901 aggaaaatat gccattgttg gtactgatat tgaaagttag ttaatttgtg ttgtctctgg
     6961 ttaacaagcc ggaacgcagg gcagggaatc ctcctgaccg ggcgggaaac accggacaca
     7021 gaatttttta tgatgaatta cagcgaaaca ctggattttt tatacagccg tcttccggca
     7081 tatcaccgca taggcagggc tgcctacaga aacaatcttg acaacaccct tgcccttgat
     7141 gcatatttcg gacatcctca ccgcaattac ctttccgtac atattgccgg aaccaacggg
     7201 aaggggtcgg tttcgcacat gcttgcatca atactgatgg aagccggata taaaaccggt
     7261 ctttacactt caccccatct gaaggatttc agggaaagga taaggataaa tggtaagatg
     7321 atcccgaagg gccgggttgt ttcctttgtc agaaatcaca gggggatcat tgaaactgtt
     7381 cagccctcct tctttgaact gacggcagca atggcatttg attatttttc gcgtgaaggg
     7441 gtggatgtat cggttattga aaccgggatg ggggggaggc tggattcaac caacatcata
     7501 acacccgttc tgtcggtcat tacgaacata gggcatgatc acgccgatct tctgggagat
     7561 accccggaaa agatcgcggc agaaaaggca ggaatcatta aaacaaatat tccggtggta
     7621 ataggtgaaa cacagtctga aaccatggaa atattctgtg aatctgcaag ggcgtcccat
     7681 tcccgtattt tttttgcgga caggcattac aggtgtactg ccacagtaga ttccgctgat
     7741 ccggaattaa gatactatac cgtggagaga ccggcaggag gggaagcgtt tacgggtgag
     7801 gatccgtgca atcgcggaaa tgcactcacc tccgtgaatc cgcccacttc cgggaatcca
     7861 tcagctgccg tgaaacgggt aaatgttgct gttccgcttg tgggggatta ccaggtcaga
     7921 aatattcaga cggtgtttca ggcctgcgaa tgtctcagtg attcactggc aattgatgaa
     7981 aaagctgttg tcggtggtct gaggaaggtt gtcagcaata ccggtttttc gggccgctgg
     8041 cagatattgc agaaaaggcc actggtcatt gccgataccg gacataacag ggagggcatt
     8101 gagtatgtgg tacgtcagat tggcaaaatg aatgcagaac ggatacattt tgttctgggt
     8161 tttaccggcg acaaggatct ttctcttgtg cttccgcttt ttcccgtgaa tgctttctat
     8221 tattttacca gggcatcggt ttcacgtgct ctggatgaaa aactgcttca gaaagaggcc
     8281 ctgaggtacg gactgagggg cgaaagttat ggaacagtgg ctgatgcatg gaaggcagca
     8341 ctggacaatg ctgctgcaga tgacattata tttatcgggg gcagcacttt tgtggtggcg
     8401 gatgtgttat aaatattatt taattttact gtaataaatt aattgtattt tacctgcagg
     8461 ctttattccg gcgtcggcat gccggcagaa ctgatctgat caccgaatct ttaaaaatta
     8521 ccggattatg aaaaaaataa ttgtctcact ttcgtttatc ctgcttgtgt tcagccttga
     8581 ggctcagccg gattcaaaga tagtaataat ccacagcaac gatctgcatt cccatcttgc
     8641 cggatttgct cccgagctcg attattcacc gcttgttacg ggtaatgacg gaactgtcgg
     8701 cggttttgcc aggatagcat caataatcag gagtgaaaaa gaaaaaaatg aggggatcac
     8761 ccttgttctg gatgcaggag attttttaat gggaaccctt tttcagggtg ttgaaataaa
     8821 gacaggcttt cagctgcgcc tgatgaaaac aatgggatac gatgtactct gtatcggcaa
     8881 tcatgaattt gactatggtc ccgccaggct tgccgatatc atcagttcat ctgccggtgc
     8941 gggagagatt ccggccctgc tgctcggcaa cgccgtattt gatccggctg atccggaaga
     9001 tgacgggctt gaaaaactct tttcagacaa tgtattgcga agaacgatga tcatcaacag
     9061 gggaggtatc aggttcggct ttttttccct tcttgggaaa gttgccgtcg gtaatgccgc
     9121 ctatgctcct cctgttaagt tttcaaaaca gattgcctct gcccgtcaga tggttaagga
     9181 gcttaaggat gaaggctgtg atgtgattat ctgtctttca cattcgggag tatcaaggga
     9241 taaaaagggc agatgggcag gtgaggatgt cgaattggcc aggaaggtaa aaggcattga
     9301 tgtcatagta agcggccata cccacactgc cctggagcag cctgtaatgg tcgggggtgt
     9361 acccgtagtc cagacaggtg agtatggcat gaatatgggg aaactgacac ttgaatataa
     9421 aggaggaaag ctaaaggtaa gcgattattc gctgataccc gtcaatgaca atattgcagg
     9481 cgacaggcat atccatgatc ttattgagct gcaaaaggag agggttaatg aagatatact
     9541 gaagccggcc ggactgagtt atgacaaacc ggttgttgaa actgatttcc ttcttgaatg
     9601 tgacgaagga ggtgacataa gaaacagtaa tctcggcccc ctggtggcag atgcgattca
     9661 cagctatgtg aacaatcatg tcaggacagg aactgatatc agtatggtcg ccgttggtgt
     9721 catccgcgac aggattgttc cgggttacca gtctgcaccc gatattttca ggataatgtc
     9781 gatgggaaca gggaatgatg cgattccggg ataccctctt tccagggttt accttacagg
     9841 aaaggagctt aagagtgttc tggaaatact ccagcttgcc tacaggtcaa ctccggagaa
     9901 ttattgttat tattccggac tgagagtcat gcacgatccg ggcagaatga tgttcagaaa
     9961 aataaaaaga attgaactgg taaaggccga cggaaatgta aaacaggtgg atttctcaag
    10021 gaaaaacaag gaactttttt cggtaacggc caattcctat atgctggaat ttgtcggtat
    10081 aataaaaaag atgagctttg gcctgttgaa cgtggttccg aaagatatta acgggaatcc
    10141 gcttactgac atgaaaaagg cgattcttga ctttgatgaa aacacacccg ggattcagga
    10201 aggcagggaa tggctggctc ttgtggaata cctgtcatcg atggaagaca ctaatttcaa
    10261 caatattccc gatatagaca gtaaatacag ggaagcaatc cagactttta taccggtaaa
    10321 gtgaattgtt gccgcctcac catttgctga atggattatt tactttcagg cgttttttgt
    10381 tatttgccac tgtatagccg atctggaaaa gagcttcggt tattctctcc attttcccga
    10441 aattaatttt tccaatcaca tctccgggtg tatgataatc ggtatgaagg cctgttgtga
    10501 aaaagagtac aggaatatcc ttcctaacga aattatagtg atcgctgcgt gcaaaaagct
    10561 gaaggggatg atctcttccg ctgagggaat aatcgaaatc cagagggata cgttcatcca
    10621 catccctggc tattcctgac agttccctgc tctggtttcc cgttatcaca aacacctcgt
    10681 taagtcccgt catgggatag tctgaagtag tgtccgctat gcctttcacc ctgccgatca
    10741 tgtcgacgtt gatatctgca act
//
LOCUS       DAOSPX010000074        10703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6037_length_10703_cov_3.593914, whole genome shotgun sequence.
ACCESSION   DAOSPX010000074 DAOSPX010000000
VERSION     DAOSPX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6037_length_10703_cov_3.593914"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLZ75_05330"
     CDS             <1..614
                     /locus_tag="PLZ75_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOS71853.1"
                     /translation="FMLISFPVQMTSWPVPAGFQTGYSDAVTGATPLAIIKEGIKNGE
                     PVSQLMSQIPPPSQMFLGDIGGSMGEVSAFFLILGFIWLLYKKIITWHIPLSIILTII
                     VFTGTFWLINPEKYADPLFHILAGGVLLGAIYMATDYVTSPMNPKAMLIYGCGIGVLT
                     SVIRIWGAYPEGVSFAILIMNAFVPLMNRYIKPKRFGEEIKNG"
     gene            607..1182
                     /locus_tag="PLZ75_05335"
     CDS             607..1182
                     /locus_tag="PLZ75_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOS71854.1"
                     /translation="MAKSESTLKNMMLALTLIATVSAAGLGFVYELTKEPIALSVLNK
                     KLNAIREVVPEFTNNPDDEMFRLATGEGDSLEVYPAKNNGEIVGYAVNTYTNNGFSGY
                     FSVMAGFRPDGSISGISVLEHKETPGLGTKMAEPGFRNQFTGRNPGESILKVRKDGGE
                     IDAITAATISSRAFCEAVQRAYNTLEKGGLK"
     gene            1179..1766
                     /locus_tag="PLZ75_05340"
     CDS             1179..1766
                     /locus_tag="PLZ75_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOS71855.1"
                     /translation="MSQLQNFTKGFIRENPLLVLVLGTCPALATTTSSLNGMGMGIAT
                     TFVLICSNVVISLLSDLIPDKVRIPAFIVIIASFVTIVDLIMQAFVPDLYQALGIFIP
                     LIVVNCIVLGRAEAFASKNKTLTSLIDGAGMGLGFTMALGLLGAMREILGEGAIFGYK
                     FIKSDGILVFILAPGAFIALGYVIALVNKIRKTSK"
     gene            1772..2347
                     /locus_tag="PLZ75_05345"
     CDS             1772..2347
                     /locus_tag="PLZ75_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOS71856.1"
                     /translation="MEYILIIISAIFINNIVLAQFLGVCPFLGVSSKVNTAVGMTGAV
                     TFVMVLATMSTYLIQTYVLEKLGIAFMQTITYILVIAFLVQIVEIILKKISQPLYQAL
                     GIFLPLITTNCAVLGVAILVIKNNYNLLESVVYSAATATGFGLALLILAGIREQLDLV
                     NVPEGMKGVPVSLVTAGILALAFMGFTGIIR"
     gene            2388..2948
                     /locus_tag="PLZ75_05350"
     CDS             2388..2948
                     /locus_tag="PLZ75_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOS71857.1"
                     /translation="MNYPEYFKILGLSTDATIPEIKKAYRLKARMFHPDLNNSPDARE
                     NFIMVTEAYEFLIENFDRLKNDHAFSVALEEWKRSMQNLSRQRARAYAHESYRQFRNS
                     RLYRTTKFFDLTRIIFGLILSVMILVFTILGFITKQKSPIPEQGNPVIIFIMLLFLGL
                     VFLVASLVFLKVYLEDTGKHKKKHKG"
     gene            complement(3183..3557)
                     /locus_tag="PLZ75_05355"
     CDS             complement(3183..3557)
                     /locus_tag="PLZ75_05355"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOS71858.1"
                     /translation="MKELKIPLTKTSPEIILSPDGLIKIRGRSIQENVGEFYSSVEKW
                     MNEYIDTPADITCVEMNLEYFNSASARVFIQLLEKIKRVTLKNKKFVVNWYYEEGDED
                     ILERGEYFSSVLDIPFNFFMIS"
     gene            complement(3607..4638)
                     /locus_tag="PLZ75_05360"
     CDS             complement(3607..4638)
                     /locus_tag="PLZ75_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOS71859.1"
                     /translation="MLGKDLSILVIGAGAIGGITAAFLKKNGYNVEIISKYEDYATLI
                     REQGMEISGISGSFTVTVPAYASVDEVKGKKDLILHATKVNDMVEAARSVLPVLSENG
                     YLVSLQNGICEDVLAGIVGQERIIGCVTGWGATMEARGRLVMTSTGDFILGYPDKEAD
                     DDLKTVADILSRVVPVRISDNIMGHLYSKLIINANITSLGVICGLDLGKMLAIKKIRN
                     IFIEVIREAVSLADKINIKIEVFGGRLDFRKFISGNSILADFRRHMLIRIIGFKYRRL
                     KSSSLQSFERGKPTEVDFLNGYIVRNAEKTGVDVPVNRAILNMIHEIEQGRRKISPDN
                     FDDPVFDRF"
     gene            complement(4657..5670)
                     /gene="tsaD"
                     /locus_tag="PLZ75_05365"
     CDS             complement(4657..5670)
                     /gene="tsaD"
                     /locus_tag="PLZ75_05365"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOS71860.1"
                     /translation="MGVTILAIESSCDDTSAAVVRDGYVLSNLIAGQEVHRSYGGVVP
                     ELASRAHQANIVPVVDQALKRAGTDISEISALAFTRGPGLLGSLLVGASFAKGFALAR
                     NIPLIEVNHLQAHVLVHFIKRKEENRPAPGFPFLCLLVSGGHTGIMLLKSHLEMETLG
                     STIDDAAGEAFDKCGKLMGLPYPAGPEIDRLASQGNKNAFKFSKPSVPDLNFSFSGLK
                     TSFLYFLRDRLKEDPDFIARNKADLCASLQAVIADILIEKLIAASKATGIREIGIAGG
                     VSANSGLRNRAREAARERGWKLFIPDPGLTTDNAAMIGITGYYKYLGKEFAGQDIAPQ
                     ARL"
     gene            5865..10334
                     /locus_tag="PLZ75_05370"
     CDS             5865..10334
                     /locus_tag="PLZ75_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOS71861.1"
                     /translation="MIRAVNRTIRILLILAGTIIMIPALLYPVLRHPEIQTLIIKRIT
                     RHFSQELKSTISVGKTEFIFFNRLSMDDVLIKDKNDDTLLYAKKLSVTIRRINTERNE
                     YTLGSVVIMNPVFSLITDTAGVQNLQWYLALLKRGSGKPAGQPVALKINHADIRNGSF
                     SLINQGTEIRENNTIDFNRLHLGSINGNLREFSVRKDTTSFSVHDLSFRESGGFTVRK
                     MSSDFAVADRNIIFTSANINCDSSILNIPLVELKAGPDGTFGNFINTVNLDIQIDKSL
                     VYTSDLQHFVAAVNGMHENVNIRGRIFGTIAELRGRDISLGSKEETVLDCDFDLSGLP
                     DIMNSFIYLGVHQLKSSAEDIKDIRVPGGKALRLPDYFERLGKISFNGSFTGFTTDFV
                     AYGKLESEAGNLNTDVSFRPEGKNQFKIKGLITGNGINLGEITGNSDLLGDLSIKTDV
                     DMHACSFKTFSGEINGLIDSVEINHYKYRNISLNGIINDKTWDGSIKIADENIRLDML
                     GMMNFNRELPEFDFTLNLVKADLFRLHLDKSDSTSAASMLLTANFRGKIDENLVGEVK
                     LLNSSLRKYGNTLDMYDFSLRSFMDNNQPSVSLRTDYVDADLKGRFNPAGLMSFIASA
                     KAGLMPSRYVRPELQSPFVTDTLVFAVNFKQTDNINNFFRTGLLLADKSTITGEIVAD
                     TAAHAILLSDKLVFRGNTFSDLAVDLDYSRFLLGAGLRSSSLNFLGHSDLEDFSADFT
                     VKPDNFIFSLNWEGNDPRSAGSGSFTARGISMKEAGMGAGPLMLIDIASSDIINPDNQ
                     WRINHSTILLDSNMVNINNLLIHNESSYYLIDGTLSKNREDSLRLEFKNIDISPLNSL
                     ISGKDDREKLSLDLKGELSGKVLLTDVYSNPLAEGDLRINGFSILGGEYGTLTLNSAW
                     NNEGKVAEIRAGNNLSGKKMLDVKGYYNPQARQLDLTASSEKLPVDALNPLLNSFASD
                     ISGSASGKVRISGELNRLVMKGSLMAQNTSMTIDYLQTRYKLNDSVRFDNNKIIFRDL
                     KLSDERGNTAMLNGKVFHNHFKDFNADITVSFNDFMVINTKARDNEDFYGTAFATGIV
                     TIKTGQNLISFDISARTGRNTRFYMPLNTSETLTDYSFVSFVTPGTSGVASDLPGSAA
                     TALAQATTGIELNFDLDVTPDAEIQLIFDEKVGDVIRGRGSGNLNINLDQKGNLGISG
                     DYVIDNGDYLFTLGNVMNKPFTVENGGKVSFSGNIDNAEIDLKAIYRLKASLFEILQD
                     DRFNERIPVECQILLSGKLFNPVVGFNIDLPSADEERRAYLRNVITTDEELSRQFLYL
                     LVMNSFYADPSYRASSGVTGTGTSAMAVTTTEMLSNQLSNWLSQISNDFDIGFLYTPG
                     TKDLSSQQLQVALSTQLLNDRVTINGNFDVRSGTETEETPLIGDFDIEYRLTEKLRFK
                     VFNRYNNPYTGKGAPYTQGLGFSLRQDFNRFRDLFRRKPPVEFRKEEEVGIRE"
     gene            10478..>10703
                     /locus_tag="PLZ75_05375"
     CDS             10478..>10703
                     /locus_tag="PLZ75_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOS71862.1"
                     /translation="MGFLQLLMQEGQEATTMAAEKLTLLDFAIKGGWVMIPIVLLFLV
                     ATYIFIERLYVIKQASKQDFNFMNRIKDYIH"
BASE COUNT         3023 a         2510 c         2393 g         2777 t
ORIGIN      
        1 tattcatgct gatatcattt cccgtacaga tgaccagctg gcctgttcct gcaggatttc
       61 agacaggata ctccgatgcg gtaacaggtg caacaccgct ggcgataatc aaggaaggga
      121 taaagaacgg ggagccggtt tcacagctta tgtcacagat acctccgccc tcacagatgt
      181 tccttggaga tatcggtggg tcaatgggtg aagtgtccgc ctttttcctc atactgggat
      241 ttatctggct gctgtataaa aagattataa cctggcatat accactgtcc attatactga
      301 caataattgt tttcaccgga acattctggc ttatcaatcc ggagaaatat gctgatccgc
      361 tgttccatat tctcgcggga ggtgttctcc tcggggctat atacatggca accgactatg
      421 ttacctcacc catgaatccc aaagccatgc tcatctatgg ctgcgggata ggcgtgctta
      481 cctcggtgat cagaatatgg ggggcctatc ccgaaggagt ttcatttgca attctgataa
      541 tgaatgcatt tgtgcctctt atgaacagat atatcaaacc aaaacgattc ggggaggaga
      601 ttaaaaatgg ctaaatcgga atccacactg aaaaacatga tgctggcact tactcttatt
      661 gccacggtaa gtgccgcagg actgggattt gtgtatgaac tcacgaagga acccattgcc
      721 ctttcggtcc ttaacaagaa gctgaatgcc atcagggagg ttgttccgga attcaccaac
      781 aacccggatg atgaaatgtt caggctggcc acaggtgaag gggacagcct tgaggtatat
      841 ccggcaaaaa acaacgggga aattgtgggc tatgctgtca atacctatac caataacgga
      901 ttcagcggtt atttttcggt gatggcagga ttcagacccg acgggtcaat aagcggcata
      961 tcagtgcttg agcataaaga gactcccgga ctgggaacca agatggcaga acccggtttc
     1021 aggaatcagt tcaccggcag aaatccgggg gagtcaattc tgaaagtaag aaaggacggc
     1081 ggtgaaattg atgccatcac tgccgcgaca ataagttcaa gggcattctg cgaagctgtt
     1141 cagcgtgcat ataacacatt ggagaaagga ggattaaaat gagtcagcta caaaatttca
     1201 ccaagggttt tatcagggaa aatccattgc tggtcctggt gctaggtaca tgccctgccc
     1261 tggcaaccac cacttcatca ctcaatggca tgggaatggg catagccaca acctttgtgc
     1321 tgatctgttc aaatgtggta atatccctgc tgtcggacct gatacccgac aaggtgagga
     1381 taccggcatt cattgtcatc atagcctctt ttgtgaccat tgtcgacctc atcatgcagg
     1441 cctttgttcc ggatctctac caggccctcg gaatattcat acctctcata gttgtcaact
     1501 gcatcgtcct gggaagggct gaagcatttg ccagcaagaa caaaacactt acctccctta
     1561 ttgatggtgc cggaatgggt ctcggcttta ccatggccct gggattgctc ggagccatga
     1621 gggaaatact gggcgaggga gctattttcg gttataaatt cataaaaagt gacggaatac
     1681 ttgttttcat tcttgcaccc ggtgcattca tcgccctcgg atatgtgatt gcactcgtta
     1741 acaaaatcag gaaaacctca aaataagagc catggagtat atcttaatta tcatatccgc
     1801 catattcatc aacaacatcg ttcttgccca gttcctcggt gtatgccctt tcctgggcgt
     1861 ttccagcaag gtcaatacgg cggtgggaat gacaggtgcc gttacattcg tgatggttct
     1921 ggcaaccatg tccacctatc tcattcagac ctatgttctc gaaaaactgg gcatagcctt
     1981 tatgcagaca atcacctata ttcttgttat tgcctttctt gtccagatag ttgaaataat
     2041 cctgaagaaa ataagccagc cgctctacca ggcactcgga atattcctcc cccttattac
     2101 aaccaactgt gcggttcttg gcgtcgccat ccttgtcatc aagaataatt acaaccttct
     2161 cgaatcggtt gtttacagtg cagcaacagc aacaggtttc ggcctggccc tgttaattct
     2221 tgccggaata agggagcagc tcgacctggt gaatgtgccg gaaggaatga aaggagtacc
     2281 tgtttcactg gttacggcag gaatacttgc acttgcattc atgggcttca cgggaataat
     2341 tcgttaattg tgtaaaattg ataatgaggg gaaataacag gaattaaatg aattatccgg
     2401 aatatttcaa gatccttggt ttgagcactg atgccacaat tcctgaaata aaaaaagcat
     2461 acaggctcaa ggcccgtatg ttccaccctg acctcaacaa ttcccccgat gccagggaaa
     2521 attttattat ggttaccgaa gcctatgaat ttctgattga aaatttcgac aggctgaaga
     2581 atgaccatgc cttttcagtt gcccttgaag aatggaaacg atccatgcag aatttatcaa
     2641 gacagcgggc aagggcatat gcacatgaat catacaggca gttcagaaat tccaggcttt
     2701 accgtaccac aaagtttttc gaccttacca ggatcatctt cggactgatt ttatcggtaa
     2761 tgatactcgt ttttacaatc ctcggattta ttacaaaaca aaaatctccg atacccgaac
     2821 agggaaaccc ggttataatc ttcattatgc tcctgttttt aggtctggtg ttcctggttg
     2881 ccagcctggt ttttctgaag gtttaccttg aagacacagg aaaacacaaa aaaaagcaca
     2941 agggctgaaa gactgtaaac tccggataat gtttccgcag gagaaaataa atcaccggga
     3001 aaataatcag cagcaggcgg aatagtatta cggtgccacg ggaatactga ataacatcat
     3061 tgtcctgtca ggtcaacagc cttgccgcaa atcataaacc tgccggaaac cataagcctg
     3121 ccgcaaacca caaacctgcc gcaaatcata aacctgccgc aaataatgaa tctgccggat
     3181 tatcagctta tcatgaaaaa attgaagggt atgtcaagga ctgatgaaaa atactcaccg
     3241 cgttcgagga tgtcctcatc gccctcctca taataccagt tgaccacgaa ttttttgttc
     3301 ttgagggtca cacgcttgat cttttcaaga agctgtataa aaacccttgc actggcactg
     3361 ttgaaatatt caagattcat ttccacacag gtaatatcag cgggtgtgtc gatatactca
     3421 ttcatccatt tctcgacaga ggaataaaac tccccgacat tttcctgaat ggaacgtccc
     3481 ctgattttta tcagaccatc cggcgacaga attatttcgg gcgaagtttt tgtaagtgga
     3541 attttcagct ccttcatctg taattagtta tccgggatat taccagcctg cttttaacgg
     3601 cgatgcttaa aacctgtcaa ataccggatc atcaaaatta tccggcgata ttttccgcct
     3661 gccctgctca atctcatgta tcatatttag tattgccctg tttaccggca cgtccactcc
     3721 cgtcttttcc gcatttctta caatataccc gttcagaaaa tccacctctg tgggcttgcc
     3781 tctctcaaag gactgaaggc ttgatgactt cagtcttctg tatttgaatc cgatgattct
     3841 tatcagcatg tgtctgcgga aatcagccag gatactgttg ccgctgatga atttcctgaa
     3901 atcgagcctc ccgccaaaga cttcaatttt aatatttatt ttatcagcca ggcttactgc
     3961 ttcgcggatt acttctatga aaatattcct gatctttttt attgcaagca ttttaccaag
     4021 gtcaagaccg catattaccc ctagtgatgt tatgtttgca tttattatca gctttgagta
     4081 aagatgcccc attatattat cgcttatcct cacgggaact accctggaaa ggatatccgc
     4141 aacagttttc aggtcatcat ccgcttcttt atccgggtaa ccgagaataa aatccccggt
     4201 tgatgtcatt accagtctgc cacgggcttc catagttgca ccccagcctg tcacacatcc
     4261 tattattctc tcctgtccca ctatcccggc gagtacatcc tcacatattc cgttctgaag
     4321 tgaaaccagg tagccgtttt cactgagaac cgggagtaca gagcgtgcag cttcaaccat
     4381 atcgttaacc ttggtggcat gaaggataag atcctttttc cccttaacct catcaacgga
     4441 tgcatacgca ggcaccgtaa cagtgaagct gcccgagatc ccgctgattt ccattccctg
     4501 ttcacgtatc agcgtggcat aatcttcata tttgctgatg atctcaacat tgtaaccgtt
     4561 ttttttcagg aaggcggcag tgatgcctcc tattgctccg gcaccaataa ccagaattga
     4621 caaatctttt ccaagcatat tatttacaat aattgttcaa agccttgcct gcggggcaat
     4681 gtcctggcca gcaaattcct ttccaagata cttataataa ccggtaatcc caatcattgc
     4741 agcattatca gttgtaagac cgggatcggg aataaaaagc ttccagcccc tttcacgggc
     4801 tgcctccctt gccctgtttc tgagtcccga attggccgat accccgccgg ctatcccgat
     4861 ctctcttatc cccgtggcct ttgaggctgc aataagtttt tcaatcagga tgtctgcaat
     4921 cactgcctga agtgatgcgc agaggtctgc cttattccgg gcaataaaat caggatcctc
     4981 cttcagtctg tcgcggagga aatacaggaa acttgtttta agtccgctga aactgaaatt
     5041 caggtccgga actgaaggtt tggaaaattt aaaggcgttt ttattgccct gtgaggccag
     5101 cctgtctatt tccggtcctg ccggataggg caggcccatc agtttgccac acttgtcaaa
     5161 tgcctcacct gcagcatcat ctattgtact gcccagggtt tccatttcaa gatggctctt
     5221 taggagcatg attccggtat gacccccgga aaccagcaga cataaaaaag ggaatccggg
     5281 tgcaggtctg ttttcttcct ttctttttat aaaatgaacc agtacatgag cctgcagatg
     5341 attgacttca ataagaggaa tattacgggc cagagcaaat ccctttgcaa aggaggctcc
     5401 gaccagcagg gagcccagaa ggcccggccc ccgtgtaaag gcaagggcgc ttatttcact
     5461 gatgtctgtc ccggcccttt taagcgcctg gtcaacaaca ggcacaatat tggcctggtg
     5521 tgccctcgat gccagttcag gaacaacccc gccgtatgag cggtgaacct cctgccccgc
     5581 tatcaggttc gaaagcacat agccgtccct tacaacagca gccgatgtgt cgtcacatga
     5641 tgattctatg gccagtatcg taactcccat ttctgcaggg ctaaaaaaag gatgattcgc
     5701 cttttatgat cctaatatat aaattatttc ttatcggcaa tccttccgcg gattactgca
     5761 caatctttgc tgtacaatat tatgaatgat atattttatt gcgctctttc agtatcttta
     5821 tgcactgaaa gtgctgagtt tttggaaagg acaggataaa acacatgatc agagccgtaa
     5881 acaggacaat aaggattctt ttgattctgg ccgggaccat cattatgatc cctgcactgc
     5941 tctatcctgt tctcaggcac ccggagatac agacactcat aatcaaacgg attaccaggc
     6001 atttctcaca ggagctcaaa tcaacaatat cagtcggcaa gacggagttc atttttttta
     6061 acaggctcag catggatgat gtcctgatca aggacaaaaa tgatgataca ctgctttatg
     6121 caaaaaaatt atccgtcacg atcagaagaa taaataccga aaggaatgaa tataccctcg
     6181 gaagtgtggt gatcatgaat ccggtattct ccctgatcac tgatacagca ggagtccaga
     6241 atcttcaatg gtatctggca cttctgaaaa gaggatccgg gaaacctgcc ggacagcctg
     6301 ttgccctgaa gataaaccat gcggatatca ggaacggctc cttttcactc atcaaccagg
     6361 gtacagaaat cagggaaaac aataccattg atttcaacag gcttcacctg ggcagcataa
     6421 acggaaacct cagagaattc tcggtaagga aggataccac atccttcagt gtccatgatc
     6481 tgtccttccg ggaatcagga ggtttcacag tccgcaaaat gtcaagtgat tttgcggttg
     6541 cagacaggaa tatcatattc acttcggcaa acatcaactg tgacagcagc attctcaaca
     6601 ttcccctggt tgaactgaaa gccggtccgg acggcacttt cggcaatttc ataaacacgg
     6661 tgaacctgga tattcagatt gacaagtccc tggtatatac atccgacctg cagcattttg
     6721 tcgccgcggt gaacggaatg catgagaatg tgaacatccg gggaaggatt ttcggtacaa
     6781 tagctgagct caggggccgc gacataagcc tgggttcaaa agaagagacg gttctggact
     6841 gcgatttcga cctttcgggt ctgccggata taatgaactc attcatttac cttggtgtac
     6901 atcagcttaa atcaagtgct gaagatatta aggacatcag ggtgcctggg gggaaagcac
     6961 tgaggcttcc cgattatttt gagaggctgg gaaaaatatc ctttaacggc agctttaccg
     7021 ggttcaccac ggattttgtc gcatacggaa aactggagtc ggaggccgga aacctgaaca
     7081 cggatgtctc attcaggccc gaaggaaaga accagttcaa gatcaagggg ctgataacag
     7141 gcaatggcat caatctgggg gagatcacgg gaaacagcga cttgctggga gatctcagta
     7201 ttaagacgga tgttgacatg catgcctgtt ccttcaaaac attctctggc gaaataaacg
     7261 gactgatcga cagtgttgaa ataaaccact ataaatacag gaatatctct cttaacggga
     7321 taatcaacga caagacctgg gatggcagta taaagatagc agatgaaaat atcaggctgg
     7381 atatgcttgg aatgatgaat ttcaacaggg aactgccgga atttgatttc actctcaatc
     7441 ttgtaaaagc cgatcttttc aggcttcacc ttgacaaatc cgacagcaca tcggcagcat
     7501 caatgcttct gacggcaaat ttcaggggaa agattgacga gaaccttgtg ggcgaagtga
     7561 aactgcttaa ttcaagtctc cggaagtatg gcaatacgct cgacatgtac gatttctccc
     7621 tcaggtcatt catggataac aaccaacctt cagtcagcct gagaaccgat tacgtggatg
     7681 cagatctgaa aggccgcttt aatcctgcag gactgatgtc atttatcgct tcggcaaaag
     7741 ccggactgat gccttccagg tatgtcagac cggaattgca aagccccttt gtaacggata
     7801 cacttgtatt tgccgtgaat ttcaaacaaa ccgacaatat caacaatttc ttccgaacag
     7861 gtctgctgct tgcagataaa agtaccatta caggcgaaat agtggccgat accgctgcac
     7921 atgcaatact tctttcagat aaactggttt tcaggggcaa tactttcagc gacctggctg
     7981 tagaccttga ttattcccgg tttttactcg gagcaggact caggagctca tcactcaatt
     8041 ttctcggaca ttctgacctc gaggattttt cagctgattt caccgtaaaa cccgataatt
     8101 ttatattcag cctcaactgg gaaggcaatg acccccggtc agcgggcagc ggaagcttta
     8161 ccgccagggg aatatcaatg aaggaagcag gcatgggtgc cggacccctg atgctgatcg
     8221 acattgcttc atccgatatc attaacccgg ataatcagtg gagaataaat cattcaacaa
     8281 tccttctcga ttcgaatatg gttaatatca acaatctgct gatacacaat gaaagcagtt
     8341 actacctgat agacggtacc ctgtcgaaaa accgggaaga cagcctcagg ctcgaattta
     8401 aaaatatcga tatttccccg cttaactcac tgatcagcgg gaaagatgac agggaaaagc
     8461 tgtcactgga cctgaagggg gaactcagcg gaaaagtact tcttacagat gtttacagca
     8521 atccgcttgc cgaaggagat ctccggatca acggattttc aatactcggc ggcgaatacg
     8581 gcacgctgac actgaactcc gcatggaaca atgagggaaa ggtggcagaa atacgagcag
     8641 gaaacaatct cagcggaaag aagatgctcg atgtgaaagg ctattacaat ccccaggcca
     8701 ggcaactgga cctgacagca tcgagcgaga agcttcccgt ggatgccctt aatcccctcc
     8761 tgaactcatt tgcttcggat atttccggat ctgccagtgg caaagtaagg atatcgggag
     8821 aactgaacag gctggtcatg aagggttcgc tcatggctca aaacacctca atgacaatcg
     8881 attatctgca aaccaggtat aaactgaatg attcagtgcg ttttgataac aataagataa
     8941 ttttcaggga tttaaaactt tctgacgaga gagggaatac agcaatgctc aacggtaagg
     9001 tatttcataa tcacttcaag gatttcaatg ccgatattac cgtcagtttc aatgatttca
     9061 tggttatcaa cacaaaggcc agggacaatg aggattttta cggaactgcc tttgccaccg
     9121 gcatagtcac aataaaaacc ggccagaatc tgatatcctt cgacatctcg gccagaacag
     9181 gcaggaatac caggttctac atgccgctca ataccagtga aacgctcact gactattcat
     9241 ttgtaagctt cgttacgccg ggaacatccg gggtggcatc agatcttccc ggatctgccg
     9301 ccactgccct tgcccaggcc accaccggca tagagctcaa ctttgatctg gatgttactc
     9361 ctgatgcgga aatacagctg atttttgacg aaaaggtggg cgatgtgatc aggggccggg
     9421 gatcgggaaa tctgaatatc aatctcgatc agaagggcaa tctcggtatc tccggtgatt
     9481 atgtgattga taacggcgat tacctgttca ccctggggaa tgtcatgaac aagccattta
     9541 ctgtggaaaa tggcggaaaa gtcagcttca gcgggaatat tgacaatgcg gaaatcgatc
     9601 ttaaagcgat ttacaggctt aaggcatcac ttttcgagat acttcaggat gacaggttca
     9661 atgaaagaat tccggttgaa tgccagatcc tcctttccgg caagctgttc aatcccgtcg
     9721 tgggattcaa tattgatctt ccttcggcag atgaagaaag gagggcctac ctgagaaatg
     9781 tgataaccac agatgaggaa ctgagccgcc agttcctgta tctccttgtc atgaacagtt
     9841 tttatgccga tccgtcgtac cgtgcatcat cgggggtaac cggcaccgga acctcggcca
     9901 tggcagtgac taccacggaa atgctttcca accagttgag caactggctt tcgcagatca
     9961 gcaacgactt tgacataggc ttcctctata cacccggaac gaaggactta agctcgcaac
    10021 agctccaggt ggcgctttca acccagttgc tcaatgacag ggtgactata aacggaaact
    10081 tcgatgtaag aagcggcacc gaaaccgaag aaacaccgct tataggtgat ttcgacatag
    10141 agtacaggct gaccgagaaa ctgagattca aggtattcaa ccgctacaac aatccctaca
    10201 ccggtaaggg agctccgtac acgcagggac ttggcttctc gctcaggcag gatttcaaca
    10261 gattcaggga cctcttcagg agaaaacccc ctgttgagtt cagaaaagaa gaggaagtag
    10321 gaatacggga ataagagttg ataaatattg aaatattgtt attaacacac aataatagac
    10381 tatgccggtt ttccgttttt taatatattc gcccgcatga ctgagaatat ataacatata
    10441 attatttcat ttataataaa ttaataacag gagaaatatg ggatttctac aattacttat
    10501 gcaggaggga caggaagcca cgacaatggc cgctgagaag ctgacactcc tggatttcgc
    10561 gatcaaggga ggatgggtta tgatcccgat cgtactgctg ttccttgttg ccacatacat
    10621 cttcatagag aggttatatg tgataaaaca ggcatcaaag caggatttca atttcatgaa
    10681 caggatcaag gactatatac atg
//
LOCUS       DAOSPX010000075        10639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6072_length_10639_cov_4.591270, whole genome shotgun sequence.
ACCESSION   DAOSPX010000075 DAOSPX010000000
VERSION     DAOSPX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6072_length_10639_cov_4.591270"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1320
                     /locus_tag="PLZ75_05380"
     CDS             130..1320
                     /locus_tag="PLZ75_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOS71863.1"
                     /translation="MKIIDLKARTVAVPLNAKLRHNTGVHPGYFLRTVIEIITDEGIV
                     GLGEVGGGDQRSAFAKLKPRILGLDPFHLEIIKQKVLRSIYYLSNARLYAAIEIACLD
                     IQGKVLNRPLSDLLGGSVRERIPFIAYLFWRYDRPGGGHDRTAEDMADLCSQLNAELG
                     VKSMKLKAGVMNPREEVRILELCRKNLGDDFGLRIDPNGVWSVTTAVQMGKIMEDLNI
                     EYFEDPSWGLEGNAAVRKQIRIPIATNMYPNKFDDLGPAIRMGAVDVILTDLHYWEGP
                     RGVKDLVSVCRTFGLDVAMHSGAEFGIELAAMIHTASTIPQMKFAGDAHYHYLTDDII
                     KGGLMKYEDGCIKVPPGPGLGVELDEEKMKKYEKYYEQKGDYYARFHEDEYRPDWFPI
                     VGRI"
     gene            1331..2101
                     /locus_tag="PLZ75_05385"
     CDS             1331..2101
                     /locus_tag="PLZ75_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS71864.1"
                     /translation="MNSSGLYNLNGKTALVTGSSQGIGKAIATSLARHGADIIIHYKS
                     EDELARETVAEIRKLDQECISIKADLSKADAADFIFNECSKFKPVDILVLNASVQIRK
                     KWQEITPEEFDLQINVNLRSSLMLMQKFIDHMQEQKWGKILTIGSVQQKRPHPDMLVY
                     SASKSAIANMVKSLAVQLAPFNINVNNIAPGVFGTVRNKEALNNPVYLKKVSAKIPLG
                     YIATPEDCSALAVLLCTDAGKYITGEDIFIDGGMNIPS"
     gene            2123..2851
                     /locus_tag="PLZ75_05390"
     CDS             2123..2851
                     /locus_tag="PLZ75_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOS71865.1"
                     /translation="MSKSNVSMNLENLKSKLDDISFVELSIPEKEICERFENLYTGAV
                     NDVLREMTLMDQALPCSIMPLKFDMKKCGVAFTIRSNPDPTVGGEMDIRADMLDAMPS
                     GCMVVWDAGDELEASHWGEVMTASAIARGARGAVINGGIRDTIQVLEQKFPIFYRYRT
                     SNGSLGRTKITSFNRPLRIGKAYIKPGDFMFGDIDGVVVIPRNIAEKVLTRAEEVKLG
                     EKEIRSWVKKGFSAKGIVKDGGYF"
     gene            complement(2859..4112)
                     /locus_tag="PLZ75_05395"
     CDS             complement(2859..4112)
                     /locus_tag="PLZ75_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS71866.1"
                     /translation="MKKIRIPYKWELIILLWFAFFLNQADRQIFNIVLPSLRDDLGLS
                     DADMGLIASIFTLFYGLMVPVGGILGDRFNKKYILIGSLLVWSAATLITGLSYTLVQL
                     IILRSIALGGGEAFYAPSANSLICEHHQKTRATAISIHQTALYVGIIGSGYLTGYIAD
                     NFGWRTAFYLFGGFGIILGIIFYFRIKDSKVLLKEIAITENRQSVWEVIKIFFKKPTA
                     VFLALAFASFQFGGVGFLTWMPTYLYENFHFSLARAGFDSTFYHFVAAFFGVMAGARI
                     SDIFSSRIVGFRGMVQMTGLFIMVPVVFFMTKSDSILIVYLTLTVFGFCRGIYDSNIF
                     AALYDVIEMPYRSTATGVMLMFAFVVGSLAPYLLGLLKPVFGLSNGLASLSLSYLLAG
                     IFILIALLFSYKKDSIKEELLQADA"
     gene            complement(4169..5266)
                     /locus_tag="PLZ75_05400"
     CDS             complement(4169..5266)
                     /locus_tag="PLZ75_05400"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOS71867.1"
                     /translation="MKRLVSLDALRGFDMFWIMGGEGIFSGLAIITGWPVLKWWAGQL
                     THVPWHGFHFYDMIFPLFLFIAGISFPYSYIKRIANGKSRSSIIWHVIYRGLLLVFIG
                     ILYNNGLSFNFSELRYGSVLGHIGVAWMLSAIIFMNTRLTGRIIWCFGVLIGYWLLLL
                     LFPAPDLGSSDPFSLEGNFAGYIDRLITPGKLYLGVSEPSGFSTVLPSIGTALLGMFT
                     GEYILSKRDAGKPMKKVLYMFLASVILMITGKIWDIFFPINKMLWSSSYVCWAGGLSL
                     LLFVIFYLIIDVWGYRKWAFFFVVIGMNPITIYLATKIISFSHATDFFFGGFTRIMSE
                     NWTELINGLGLTTVGWFFLYFLYKKKIFLKI"
     gene            complement(5313..6332)
                     /locus_tag="PLZ75_05405"
     CDS             complement(5313..6332)
                     /locus_tag="PLZ75_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HOS71868.1"
                     /translation="MADRRDFIKQMAAGAVGASMPGFLATDKKKENEKIWACLLHLSF
                     NMWEEYVSPHRPFRGYDPNMRLSESLWDDSIRRMSEKGMNMIVIDLGNAVRYQSHPEI
                     AVNNAWSIERLRSELQKMRKLGLEPVPKMNFSTGHDTWLGEYAKMVSTEKYYQVCADL
                     ISEVCDIFDKPRFFHLGMDEENAGHQSHYKYIVVRQGDAWWKDFFFLVEKVENKNSRA
                     WIWPDYMLWNYPDEFLSKMPKTVVQSNWYYSEDFSGGKYVSAYHQLESAGYDQIPTGS
                     YHAQNELSIINTTKFSASNIDDKRFLGILQTFWKPTIEEFRERILKGIDLAGEAKQWY
                     IKNRR"
     gene            complement(6374..7450)
                     /locus_tag="PLZ75_05410"
     CDS             complement(6374..7450)
                     /locus_tag="PLZ75_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71869.1"
                     /translation="MRPVKIAMLSLTHGHTRKYYQTLNDNPKLDWVAVYAEDETVRKN
                     FLAWGYNVPCYLTEKEMFEKHPDIEAVVMASANIKHIGQFRECAKRGIHVLCMKIPTF
                     DMQEYDEIISLVKESGIVCQVELEMHYNPVVKRLYELAAANVLGKISSINATNITLSP
                     IWAFPWQGIPEDSYGKRIKLKKGDHRFRGGALADHPHIFDLIRWMTGSDFDFIYAEAG
                     PNIRKDIQVEDNLLVNGKMKNGVSFLLDPSWSRLEERLKVPAPGWEIFPKRMEVNVTV
                     SGENGIMLVDCFGPNVYHNGKPNDRYTVQYTYFDEWIGLMDEFVSCIREKKTPKINLE
                     WHRKTIQAMNACYDSISKGNVVHI"
     gene            complement(7466..8230)
                     /locus_tag="PLZ75_05415"
     CDS             complement(7466..8230)
                     /locus_tag="PLZ75_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71870.1"
                     /translation="MIKQNMNVEEALAGLLIKYEIKPVKPFHLEKKKATEGLVLLPGY
                     EFIEQVTGDNEIPLLPWRLRRKFVELKKIVDDSVIEDACLYRFCCMGSKDKWTLASLF
                     YRELDLFEFIGNSRVTAVNTIMADNEAGSAIATLESGVICSIEVSTQVPPGTVMVDRH
                     EIIASRGVASDQPVDTQVPMSSIYCYTKNGENRFTDTDFELFDLDDLQIDQVRSAFQV
                     LKNPDLGKQWHKQHTHLMQLVQAVFESDKKHEKIKI"
     gene            complement(8244..8999)
                     /locus_tag="PLZ75_05420"
     CDS             complement(8244..8999)
                     /locus_tag="PLZ75_05420"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOS71871.1"
                     /translation="MKTNDAGYLNQVIDLLKKVEEREADNIDKGAELMAKAIEKDELI
                     HVYGGGGHTTLVMGEMFFRAGGLANINPIMEPGLSVFNQALKYLELERTVNYGRAIVK
                     YFKIKKGEVLIIFHNIGINPATIDAAMEAKESGAQIIAVSSSFWQNEMAKDHFIRHPN
                     KKNLFDYADVCIDDYNPVGDAVVKLDGFSTSIAPISNIIDFYIAHRLEISAIRKCVER
                     GIKPPVWSSANAPGGDEINAKFVEKYLPRVKYL"
     gene            complement(9016..10065)
                     /locus_tag="PLZ75_05425"
     CDS             complement(9016..10065)
                     /locus_tag="PLZ75_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71872.1"
                     /translation="MSVIETGVSYYGLSYPEHAEKDFKEMIRHNCNAVVLALTEFDID
                     FWFPNIIRITKAAKDLGMKVYLDTWGIGKWFGGEPPSLFLTNNTGNRQVSAFSNELLP
                     AVCFNTKAFREYFYGICEKLATNVDADGFFWDEPHYALPKSYASITGGAGDDWSCRCE
                     YCKREFQQQYGYEMPKLMTREVINFRQNNALEILRVASQRIKNIKPDSKIICCVHATI
                     NTYYVKENRGYDDWDKVGKVDVFDVFSTTILSYKLPRSFFKSITRETVDIARKYGKGS
                     QRWLMNYYQEPDNLAEIKEIVHMYDDMGVESLFAWTYRGGYGTVLAAPNALEMWNMLG
                     EAYGEVLDKKYSLRT"
     gene            complement(10077..>10639)
                     /locus_tag="PLZ75_05430"
     CDS             complement(10077..>10639)
                     /locus_tag="PLZ75_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71873.1"
                     /translation="TSMIKGSFQMEGWRVSNLSRSEIRKTSKLSRELLTCLGDVLAMS
                     DDFSMNASFLRLSEAKELNGVKPLLNPHTELTLKGNAENGYCRSHQYELVKYVYGPEL
                     DAYWTYVFKKISSGDRSHWKMPAEFGDQYKVIADKFYNTPLSAMAPALPRNPAKLKEI
                     LLRMEKLVNDLLLQNPETITNLNIKN"
BASE COUNT         3149 a         2314 c         2134 g         3042 t
ORIGIN      
        1 tctaggttta cctgggaaat gaaaaagaaa attattgtga atatcaggga gtcagctctt
       61 aagataatgg aaaaacttaa tgctcatact aatccctgag aattgaatat ttgttaaacg
      121 ataatagtta tgaaaatcat tgacttgaaa gccagaacgg tggctgttcc actaaatgca
      181 aaactaaggc ataatacggg tgttcacccc ggttactttt tacgcactgt gattgaaatt
      241 attactgatg agggtattgt aggtttagga gaagttggag gaggagatca gcgcagtgcc
      301 tttgccaaac tcaaacccag aatacttggt cttgatcctt ttcatcttga aattatcaag
      361 caaaaagtat tgcgcagcat ttattatctc tccaatgccc gtctgtatgc agcaattgaa
      421 atagcctgtc ttgacataca agggaaagta cttaaccggc ccttaagtga tctgcttggc
      481 ggcagtgtcc gtgaaaggat tccttttatt gcatacctgt tctggcgata tgaccgcccc
      541 ggcggaggac acgacagaac agcggaagac atggctgacc tttgttcaca gcttaatgcc
      601 gagcttggtg ttaaatcgat gaaacttaaa gccggtgtta tgaatccccg ggaagaagtg
      661 aggatactgg aactatgcag gaagaatctt ggtgatgatt tcggattaag gattgatcct
      721 aatggggtat ggtctgtgac aacggcggta cagatgggga agataatgga agatcttaat
      781 atcgaatatt ttgaggaccc atcctggggc ttggaaggta acgcagctgt cagaaagcag
      841 ataaggatcc cgattgccac aaatatgtac ccaaataaat ttgatgatct gggaccggca
      901 atccgtatgg gagctgtcga tgtcatactt acagaccttc attactggga aggcccgagg
      961 ggagttaagg accttgtttc tgtatgcagg accttcggac ttgatgttgc catgcacagc
     1021 ggggcagaat ttggtattga gcttgcagca atgatacata ccgcatcaac aatcccgcaa
     1081 atgaaatttg caggtgatgc ccattatcat tatctgactg acgatatcat aaaaggtggt
     1141 ctgatgaaat atgaggatgg atgcattaaa gtgccaccgg gcccggggct tggagttgaa
     1201 ctcgacgagg aaaaaatgaa aaagtatgaa aaatattatg aacaaaaagg tgattattat
     1261 gcccgttttc atgaagatga gtaccgccca gactggttcc cgattgtagg acgcatttaa
     1321 aacaatattt atgaattcat ccggacttta taatttaaac gggaagaccg cactggttac
     1381 aggttccagc cagggtatcg ggaaagccat cgcaacaagt cttgccaggc atggagctga
     1441 tattataata cattacaaaa gcgaagatga gctggcaaga gaaacggtag cagaaatccg
     1501 caagctggac caggaatgta tatcaataaa agccgatctt tcaaaggcag atgctgcgga
     1561 tttcattttc aacgaatgta gtaaattcaa gcctgtagat atccttgttc tgaatgcttc
     1621 tgtccagatc agaaaaaaat ggcaggagat cacacctgaa gaatttgatc ttcagataaa
     1681 tgttaacctg cgttcatctc ttatgctgat gcagaagttc atcgatcata tgcaggaaca
     1741 gaaatggggt aagatattga ctatcggaag cgtacaacag aaaagaccgc atcccgatat
     1801 gcttgtttat tcggcaagca aatcagcaat tgccaatatg gtgaaaagcc tggccgttca
     1861 gcttgcccca ttcaacatca atgtgaataa tattgcgccc ggcgtcttcg gaacggtgag
     1921 gaataaggaa gctttgaata atcctgttta cctcaaaaaa gtatccgcaa aaataccact
     1981 gggttatata gccacgcctg aagactgttc ggcacttgcc gtattgctct gcactgatgc
     2041 aggcaaatac atcaccggtg aagatatatt tattgacggc ggaatgaaca ttccatcctg
     2101 ataaccttaa aacgaaagaa ttatgagcaa gtcgaatgtt tcaatgaacc ttgagaatct
     2161 gaagagtaaa ctggatgata tttcatttgt tgaattatcg attccggaaa aagagatatg
     2221 tgaaagattc gaaaatctct acaccggagc agtgaatgat gtactccgcg aaatgaccct
     2281 tatggaccag gccctaccat gcagtatcat gcctctgaaa tttgatatga aaaagtgcgg
     2341 tgtcgctttt acaataagga gcaatcctga tccgactgtc ggaggagaga tggacataag
     2401 ggctgatatg ctcgatgcaa tgccgtcggg ttgtatggta gtatgggatg caggggatga
     2461 acttgaagca tcacactggg gtgaagttat gaccgcttct gcaatagccc ggggagcaag
     2521 aggtgcagta ataaacggag gaatacggga taccatacag gttcttgaac aaaaattccc
     2581 gattttttac agataccgta cttctaacgg atctctgggt aggaccaaga tcaccagttt
     2641 taacagaccc ttaaggattg gcaaagccta cattaaaccg ggtgatttca tgtttgggga
     2701 cattgatggc gttgtggtta tcccaaggaa tattgctgaa aaagtactta cccgtgctga
     2761 ggaagtcaag cttggagaaa aggagatcag gagctgggtt aaaaagggat tttcagccaa
     2821 agggatcgta aaagacggag gatatttcta gctgacaatc aggcatcagc ttgtaacagc
     2881 tcttccttta tactatcctt tttgtaagaa aataacaatg cgatcaggat aaatatccct
     2941 gcaagcaggt aacttaatga cagtgaggcc aagccgtttg aaaggccgaa aacaggcttc
     3001 aacaaaccaa gaagatacgg agccagcgat cctacaacga aggcaaacat caacatcact
     3061 ccggttgcag tggaacggta aggcatttca ataacatcat aaagagcggc aaaaatattg
     3121 gaatcataaa ttccacggca gaaaccaaaa actgtaaggg ttaaatagac gatcaatatt
     3181 gagtcgcttt ttgtcataaa aaacaccacc ggaaccatta tgaacagccc ggtcatctga
     3241 accatgcctc tgaatcccac gatccgggaa gaaaatatgt cagagattct tgcaccggcc
     3301 attactccaa agaaagctgc aacgaaatga taaaatgttg aatcgaatcc ggctcttgca
     3361 agtgagaagt gaaaattctc ataaaggtac gttggcatcc aggtcagaaa acccacaccc
     3421 ccgaactgaa agctggcaaa tgcaagcgcc aggaaaacag ctgtaggctt tttgaaaaag
     3481 atcttgatga cttcccatac actttgcctg ttctccgtaa ttgcaatctc cttcaacaat
     3541 acttttgaat ctttaattct gaagtagaag attatgccca gaataatacc gaagcctccg
     3601 aaaaggtaaa aagcagttct ccatccgaaa ttgtcggcaa tatagcctgt aagatagcca
     3661 ctgccgatta ttcctacata aagggcagtc tgatgaattg atatcgctgt tgctcttgtt
     3721 ttctgatgat gttcacaaat aagcgaatta gctgacggtg cataaaaggc ttcacctccg
     3781 ccaagcgcga tacttcttaa aatgataagc tgcacaagcg tataactgag acctgtaata
     3841 agggtggcag cgctccagac caacagactt ccaataagaa tatatttctt gttaaaacga
     3901 tctcccagta tccctccgac cgggaccatt aaaccgtaaa acagcgtgaa tattgatgca
     3961 atcaacccca tatcagcatc agacaggcct agatcgtccc ttaaagaagg gagaacaata
     4021 ttgaaaatct gcctgtcagc ctggttcagg aagaaagcaa accataaaag aatgatcaat
     4081 tcccacttat aaggaattct tatttttttc ataatgagct gagtttaagg caataataat
     4141 aattaattgg ttaaaaaata attaagattt aaattttcag gaatattttc ttcttataaa
     4201 ggaaatacaa gaaaaaccat cctacagttg ttaggcccag accattaata agttcagtcc
     4261 agttttcaga cattatcctg gtaaagccgc caaagaaaaa atcagtggca tgagagaaac
     4321 ttattatttt ggttgccagg taaattgtaa tcggattcat tccaataacg acaaagaaaa
     4381 aagcccattt tctatagccc catacatcaa ttatcaggta gaaaattaca aacagcaaaa
     4441 gactgaggcc tccggcccag caaacatatg aacttgacca tagcatctta ttgatgggaa
     4501 agaaaatgtc ccatatctta cctgttatca tcagaataac tgatgcgagg aacatgtata
     4561 agaccttttt cattggtttt cctgcatcgc gttttgataa aatgtattcg ccggtaaaca
     4621 tcccgagaag tgcggttcct attgagggta aaactgtaga aaagccgctg ggttctgata
     4681 ctccaagata aagcttacct ggtgtgatga gcctgtcaat gtatcctgca aagttacctt
     4741 caagagagaa agggtcactg gatccaaggt caggagcagg gaaaaggagt agcagcagcc
     4801 agtacccgat cagtaccccg aagcaccaga taattcttcc cgtgagtctg gtattcataa
     4861 agattatagc agaaagcatc catgcgactc caatatggcc aagaacgctg ccgtaacgta
     4921 attcggaaaa attaaaactc agtccgttat tgtacagtat accgatgaag acaagaagca
     4981 gtccgcgata aatcacatgc cagattattg aagatctgct ctttccattc gcaatccttt
     5041 ttatatagga atatggaaag gatattcccg cgatgaaaag gaatagaggg aaaatcatat
     5101 cgtaaaaatg aaatccatgc cagggcacat gggtaagctg cccggcccac catttcaaaa
     5161 caggccatcc cgtaataatt gccagtcctg aaaatatgcc ttctcctccc attatccaga
     5221 acatgtcaaa accgcgcaac gcatcaagcg aaacaagacg cttcaaagct tgattattat
     5281 ccatattgac ctaagataag aaccgttgta tgttatctcc ggttttttat ataccattgt
     5341 ttcgcctctc ctgcaagatc aatcccttta agtatccttt cacggaattc ttctattgtt
     5401 ggtttccaga aagtctgcag gatccccaga aaccttttat catctatatt tgaagctgaa
     5461 aactttgtag tattaataat actgagttca ttctgtgcat gataactacc ggtcgggatc
     5521 tgatcataac cggcgctctc cagctgatgg tatgctgaga cgtacttgcc gccactgaaa
     5581 tcctcagaat aataccaatt actttggacc actgtctttg gcattttcga cagaaattca
     5641 tcaggataat tccataacat gtagtcgggc catatccatg cccttgaatt cttattttca
     5701 accttctcca caagaaagaa gaaatccttc caccatgcat caccctgtct taccactatg
     5761 tatttgtaat gactctgatg tcctgcattc tcttcatcca ttccaagatg aaaaaatctt
     5821 ggcttgtcga agatgtcaca tacttcactg ataagatctg cacagacctg ataatatttt
     5881 tcagtagata ccatctttgc atattcacca agccaggtgt catgtcctgt tgagaaattc
     5941 atctttggta caggctcaag tcccaacttt ctcattttct gaagttctga tctgagtctt
     6001 tcgatgctcc acgcgttgtt gacagcaatc tcaggatgac tttgatatct gacagcattc
     6061 cccaggtcaa taacgatcat attcataccc ttttcggaca ttcgccggat tgagtcatcc
     6121 cataaggatt cactcagcct catgtttgga tcatatccgc ggaaaggtct gtggggggat
     6181 acatactctt cccacatatt aaaactaaga tggagcaggc atgcccatat cttttcattt
     6241 tcctttttct tatccgtagc aagaaacccg ggcattgacg cacctacggc tcctgcggcc
     6301 atttgtttga taaagtctct tcgatcagcc atagaatttg atggattagt taaacgttat
     6361 tgtgggcttg ttattaaata tgtacaacat taccttttga aatactgtca tagcaggcat
     6421 tcatggcctg aattgttttt ctgtgccatt caagattgat cttcggtgtt ttcttttctc
     6481 tgatgcagct tacaaactca tccatcaggc cgatccattc atcaaaatag gtatattgaa
     6541 cagtatatct gtcatttggc ttgccgttat ggtaaacatt ggggccgaag cagtcaacta
     6601 acataatacc attctctccg cttacagtca cattgacctc cattcgttta ggaaagatct
     6661 cccatcccgg agccgggacc ttcaatcttt cttccagccg tgaccaggat ggatcgagaa
     6721 gaaatgaaac gccattcttc attttcccat ttacaagcag attatcttcc acctgaatat
     6781 ctttcctgat attcggtcct gcctctgcat aaataaaatc gaaatcactg cctgtcatcc
     6841 atcttataag gtcaaaaata tgcggatgat cggcaagggc tccgccacgg aacctgtgat
     6901 cacctttttt taactttata cgctttccgt aactgtcttc aggaattccc tgccacggaa
     6961 aagcccatat tggcgataat gtaatgttgg tagcattaat agaacttatc ttcccgagaa
     7021 cattagcggc agccagctca tacagcctct taacaacagg attataatgc atttcgagct
     7081 caacctggca aacgataccc gattctttta ccaggcttat tatctcatca tattcctgca
     7141 tatcaaatgt cggaatcttc atacatagga catgaatgcc tctttttgca cactccctga
     7201 actgaccaat atgttttatg tttgcagaag ccataacaac agcttcaata tcagggtgtt
     7261 tttcgaacat ctccttctca gttaggtaac atgggacatt ataaccccag gcaaggaagt
     7321 tcttccttac tgtctcatct tcagcgtaaa ctgcaaccca gtcgagtttt gggttgtcat
     7381 ttaaagtctg gtaatatttt cttgtatgac catgggtcag tgacaacatt gcaattttta
     7441 caggtctcat aaaagtcctc ccactttaaa ttttgatttt ttcatgtttc ttgtctgact
     7501 caaaaacagc ctgtaccaat tgcataaggt gagtatgctg tttatgccac tgtttaccca
     7561 gatcagggtt cttcagaacc tggaaagcgg aacgtacctg atcaatctga agatcatcaa
     7621 ggtcaaataa ttcaaaatca gtatcggtaa atctattctc gccatttttt gtataacaat
     7681 agattgagct catcggcacc tgggtatcaa ccggctgatc gctggcaaca ccacggcttg
     7741 ctattatttc atgtctgtcg accattacgg ttccgggcgg gacctgtgta ctcacttcaa
     7801 tactgcagat tactccgctt tcaagggttg caatggcact acctgcttca ttatcagcca
     7861 taatcgtatt aaccgctgtt accctgctgt tgccgatgaa ttcgaaaaga tcaagttccc
     7921 tgtaaaacaa tgatgccagg gtccatttat ctttgctacc catacagcag aacctgtaca
     7981 gacatgcatc ttcgatcact gaatcatcaa ctatcttctt taattcaaca aatttccgcc
     8041 tgagtctcca gggcagaaga gggatttcat tatcacctgt tacttgttcg atgaattcat
     8101 aacccggtaa aagcaccagg ccttcagtgg cttttttctt ttcgagatga aaaggcttaa
     8161 ccggtttgat ttcatattta atgagcaaac cggccagggc ttcttcaaca ttcatatttt
     8221 gttttatcat aacagaagtc taattaaaga tattttactc tgggcaggta tttttctacg
     8281 aacttagcat ttatctcatc acctccaggc gcatttgcac ttgaccatac gggaggctta
     8341 atacctcttt caacacattt cctaatggct gaaatctcca acctgtgagc tatataaaaa
     8401 tcaatgatgt tagaaatggg ggcgatagaa gtgctaaatc cgtcaagttt gacaactgca
     8461 tcacctaccg gattataatc atcgatacat acatcagcat agtcaaacag attctttttg
     8521 ttaggatggc ggataaagtg gtccttcgcc atctcattct gccagaaaga cgaactgacg
     8581 gcaattatct gcgctccgct ttcctttgct tccattgcag catctatagt tgccggattg
     8641 atcccgatat tatggaaaat aataagcact tcaccttttt ttattttaaa atacttcaca
     8701 atagctctgc cataatttac tgttctctcg agttcaagat atttgagagc ctggttgaat
     8761 actgagagcc cgggttccat aataggattg atgtttgcca gaccaccagc cctgaagaac
     8821 atttctccca ttacaagtgt tgtatgcccg ccaccgccgt atacatgtat aagctcatct
     8881 ttttcaatgg cttttgccat taattcagcg cctttatcga tattgtctgc ttctctttct
     8941 tcgacttttt ttaacagatc aattacctgg ttcaggtatc ctgcatcatt tgttttcatg
     9001 gtcagatctc ctttattaag ttcttaatga atatttttta tcaagtactt caccataggc
     9061 ttcccccagc atattccaca tctcaagggc gttaggtgca gccagtacag tgccatagcc
     9121 accgcggtat gtccaggcaa acagactctc aactcccata tcgtcataca tgtgtacaat
     9181 ttcctttatc tcggcaaggt tatcaggctc ctggtaatag ttcatcagcc atcgctgcga
     9241 acctttacca tatttcctgg ctatgtcaac agtttcccgg gttatagatt tgaagaaact
     9301 ccgtggaagt ttatagctca ggattgttgt actgaaaaca tcaaacacat ccactttccc
     9361 tactttgtcc cagtcatcat acccccggtt ctcttttacg tagtatgtgt taattgtggc
     9421 atgaacacag catatgatct ttgaatcggg tttgatgttt tttatcctct gagatgccac
     9481 tctaaggatc tcaagtgcat tattctggcg gaaattaatt acctcacgtg tcatcagttt
     9541 aggcatttca tagccatact gctgttgaaa ttccctctta cagtattcgc aacggcatga
     9601 ccagtcatca cccgcacctc ctgttattga agcatagctt ttaggcaaag cataatgcgg
     9661 ttcatcccag aaaaaaccat cagcatcaac attcgttgca agcttttcac aaataccgta
     9721 aaagtattcc ctgaatgctt ttgtattgaa gcacactgcc ggtaaaagct cgttactgaa
     9781 tgccgaaacc tgtctgttcc ctgtattgtt cgtcaggaat aaactgggtg gctcaccccc
     9841 aaaccattta ccgattcccc atgtgtctag gtagactttc ataccaagat cttttgctgc
     9901 ttttgtgatt ctgattatgt tcgggaacca gaagtcgata tcgaattctg taagtgcaag
     9961 gaccacggca ttgcaattgt gcctgatcat ctctttgaaa tctttctctg catgttcagg
    10021 gtagctcaat ccatagtagc tgacaccggt ttcaattacg ctcataatga tttattttag
    10081 ttttttatat ttaaattagt tattgtttca gggttctgta acagaaggtc atttacaagc
    10141 ttttccatgc gtaacaggat ctccttcaat tttgccggat tgcgtggaag agcaggagcc
    10201 atagcagaaa gaggagtatt gtagaattta tctgcaatca ccttatactg atcaccgaat
    10261 tcagcaggca ttttccagtg ggaacggtct cctgaactga tttttttaaa tacatatgtc
    10321 cagtatgcgt ccaactccgg accatataca tacttcacaa gttcatactg atgacttctg
    10381 cagtagccat tttcggcgtt acctttcagt gtaagctcag tgtgaggatt caataaaggt
    10441 ttgacaccgt taagttcttt tgcttcagac agacgtaaaa agctggcatt catggagaaa
    10501 tcatcagaca tcgccagtac gtcgcccagg caggtcagca gctcccggct gagttttgaa
    10561 gtcttacgga tctctgaccg ggagagatta cttactctcc atccttccat ctgaaaagat
    10621 ccttttatca tggatgtaa
//
LOCUS       DAOSPX010000076        10442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6216_length_10442_cov_4.586599, whole genome shotgun sequence.
ACCESSION   DAOSPX010000076 DAOSPX010000000
VERSION     DAOSPX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6216_length_10442_cov_4.586599"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..430
                     /locus_tag="PLZ75_05435"
     CDS             251..430
                     /locus_tag="PLZ75_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71874.1"
                     /translation="MSTTRIILIVGLLLLAGFSIFQKHVRHKQGEKGNGQGKGSGKSS
                     SSEAITDDYEPYSGK"
     gene            complement(437..1720)
                     /locus_tag="PLZ75_05440"
     CDS             complement(437..1720)
                     /locus_tag="PLZ75_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOS71875.1"
                     /translation="MNKTEILHSEPVREIISDPPGKLARWGTAAISAVILIILFFAWI
                     IRYPNVVPSPVQITTENPPVTLVSKISGRILNWYVSDGETVREGRLLAVMETAASIDQ
                     VRKLKECTGEIEDPGSVSAGSLPSFSELGEIQEYWGAFLKNLSDYENYVKNDFYGYKI
                     SSVIRETAVLEEYIRRVKEKEKFYSENQRIEAKKFTRDSSLFAGAVFSESEFDISRQT
                     FNKINIELQDVRLDHAAKTLELAQKNQELQDYRIKRTEERERLLAALTESLNNLRAQI
                     RIWENTYLIISPIDGIVTFTRFWKQNQMVARDEPVLTVVPLVPGNYIGRMDLKMHRSG
                     KVRTGQAVNIKLAGYPYLEYGMVRGIVESKSLVPAGDAYIIEIILPEGLKTLYGRELE
                     FTQNMQGTAEIMTDSLRLLQKVVNPFRHLAAKNRL"
     gene            complement(1736..3922)
                     /locus_tag="PLZ75_05445"
     CDS             complement(1736..3922)
                     /locus_tag="PLZ75_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOS71876.1"
                     /translation="MSFPFVRQPDAMDCGPACLKMVASYYKKNFNLESIRRKCYITRE
                     GVSFLGLSEAANSLGFRTIGVKIPFDLLLEKVPLPCIVHWRQKHFVVVYRIRKNKIMI
                     ADPAVGLLSLSRDEFVKKWASTVEDGKPAGLVLILEPTPALLEKENEPEPVHGFRFLA
                     RYFKLYKKYFGQLMLGLLVGSLIQLIIPFLTQAIIDTGLNNNDLGFIYLILFAQLALV
                     IGRMSVEFIRGWLLLHIGARVNVSIISGFLRKLMSLPIAFFDTKLTGDILQRIDDNNR
                     IENFLTSTSLSIMFSFFNIIVFSIVLAVYSLKILALFLAGSVLYVLWVSAFMKSRARL
                     DHERFREMSVANSKLINIVNGMQEIKLTQSESLKRWDWENLQASLFSLKSKGLGLLQY
                     QTAGGTFLNEITNLLITVISATAVLKGDMTLGMMLAVQFIIGQLNVPLNQIIGFFRTT
                     QDAGMSLNRLAEVHGMEEEEADPDSKIMRLPEKKDLIISNLSFQYEGPHSPFVLKDID
                     LIIEENKTTAVVGTSGSGKTTLLKLILGFYQPVMGEILVGDTRLKNLSLRLWREKTGA
                     VMQDGFIFPETIASNISPGNSEIDDERLFRAVEMANIREFIESLPLGYNTKIGANGHG
                     LSEGQRQRILIARVLYKDPDILIFDEATNSLDANNEKAIVENLAGYFSRKTVVIVAHR
                     LSTVRNADKIVVMDKGRIVETGNHNSLIEQKGTYFNLIKNQLELGN"
     gene            4071..4643
                     /locus_tag="PLZ75_05450"
     CDS             4071..4643
                     /locus_tag="PLZ75_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOS71877.1"
                     /translation="MFKKITDSKIIAGIKAQDDNILEWIYDNYYQTVENHIIKNSGSP
                     DDVPDVFQDTIIILYRQITADSLELSSDLKAYFFGIARNLWNASLRQKNRMTGISPRF
                     DMADESDAEENEDLIFERIMSRAFYKLKPDCQTILTLYSDGYSYQEITEKMHLKSEVY
                     ARRKKYLCKEALLDLVRADSEYQEYLRFRK"
     gene            complement(4616..7705)
                     /locus_tag="PLZ75_05455"
     CDS             complement(4616..7705)
                     /locus_tag="PLZ75_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOS71878.1"
                     /translation="MPRKSVKHYSKVRFLILAGILYFSAAKYSLSSPDAFSEPGILPL
                     IEHLSNTGSGSLDRISHRIQYFSSGKDIAGLLSLADSLEKLIKGNHSDQIVQAELFYY
                     AGVCNLLSSRYENATSHLLESVRLKDSLNIADDQYLKALFNLGVAHNYLGNYSMVRRY
                     LTRYIEMSGKYYGEESPEVASAYATLIGASILNNDYKNFIEYTDRALGILKAFQQRDL
                     CDLYSSIGAGYARMGDCAKARIYFEHSESVWEKNHMEMDQNYITLINSMAINYGYLGM
                     TDKEDEYFRKGIELAVKDNSFHSFNLIHTYALAQANAGNVRRGESLLTELLERSGSYY
                     GTDSRYYIEALKNYATFLLNYRNDAEAAIKYFSVCNDYLERHPQDANLRDPVYEGYAR
                     ALHYNGRSREALAKIQELLFYGTQGKQSFDSLANPEIDSVQSDRRYIRILRSKYEILT
                     DIFAETQDIGILRAAAATSRYIIEAIDKIRLNISEEESRIVLGDHYRKSYLMAIRDSE
                     LCYRLSSDPSYLEKTFQIAEKSKVAGLLATTRELNAIQLHIPPEIADYEQSLQRKIGM
                     LNSKISLEKGKVKPNKQFLAEWNEKILVAIRARDSLILTFEKYYPEYFALKYNTVAPL
                     MTEIPAIVGKKINYISYIVSDSLLYIFVVNKKHRELVSCRIDSILFKTLGKFRELLSN
                     PALVKDARSAFVNYQETGTDLYKMLIEPVRKYFISRNLLISPDNYLSYLPFETILASK
                     YEGEGILYRKLDYLMNNFDISYAYSATLMKETGTRSISGRNRLAAFAPFYGTDINLDS
                     LFSQNQQGRGFLYDLPYARQEAEYVASIFRGNLFLNEKARESVFKKEALNFNIVHLAM
                     HTLLNDQHPMNSAMIFSQMKDDPEDGLLYTSEVYGIPLRAKMVVLSSCNTGTGILSSG
                     EGILSLARGFLYSGSRSVVMSMWEIEDKSGTEIVSRFYENLKKGHTKSNSLRKSRADY
                     LRNATQLKSHPYFWATLIVYGDNSPIYPPVRLMAVILFVLALPAAALVYYLRKRKYS"
     gene            7947..8090
                     /locus_tag="PLZ75_05460"
     CDS             7947..8090
                     /locus_tag="PLZ75_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71879.1"
                     /translation="MKNSNSSTTREIFAGYELTIDEMFNIRGGDKVKGEPIILPPSPP
                     IKI"
     gene            8287..9036
                     /locus_tag="PLZ75_05465"
     CDS             8287..9036
                     /locus_tag="PLZ75_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS71880.1"
                     /translation="MKTIDFSYFIERFLAGEMNEAEEEWFRNELGRNEKLRDEIALRQ
                     LTNTTLKNHDVLQLRNKLTAIASRKAEQTPSKKGHRHKVLTRAAVFAGLVLFGTLAFL
                     NNRTMTTDEVFDHYYKFYDITTPLRSADNAGNTDYLTAIEYFNVKDYHNAAYYFSRVL
                     SSDPRYIESVLMTGVSKFEEEKYSEAKSSFTKVMDDRDNLFFEDAQWYLALCFLKTSE
                     KDKAVSTLKAIKESGSVYSRNAVKILKKIKW"
     gene            complement(9067..>10442)
                     /locus_tag="PLZ75_05470"
     CDS             complement(9067..>10442)
                     /locus_tag="PLZ75_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOS71881.1"
                     /translation="RQSYTSTDFLNRPNYQEDFYISYLSDTGWTSPVNAGAPLNTAQN
                     EGAATLSSSGNYMYFAACDRPGGYGSCDIYFSANNNGKWSFPYNLGPPVNTSFWESTP
                     SISADGNMLFFSSNRPGGMGGKDLWYSVMNESGTWKEPVNMGDKINTEGDEMSPFIHF
                     DGRTLYFASNGRPGMGGFDIYLTRMKDDSTWTDPQNLGYPVNTAGDEMGLVIEAGGRK
                     AYFSSKRDNSSGKDIFWFTLDKSFRPNPVSYLKGRVTDRETGKMLKAEFELINLTTNR
                     VTIKSRTDDLGNFLICLPSGNNYGINVSKPGYLFYSENFMFEGTHSVMEPLIKKIELS
                     PVKIGEKMLLANVFYEIDRWELKRESMSELANLAELLRINEDFVVEIGGYTDSTGTDQ
                     HNLQLSEKRALSVVNYLVESGISPGRLQYKGYGNTSPIGDNITSEGRRMNRRTEVKII
                     ARKNEKR"
BASE COUNT         2887 a         2292 c         2188 g         3075 t
ORIGIN      
        1 aggtccctat tggaaatctc aaccatgcaa gctatcatgg ataatttaag ctacgaaaat
       61 attgaaatat ttgttaaact caaaggccag gagaaattaa tatgacaatc tgccaattac
      121 atccgtcatg gtgtgaaaga gttattttcc tatccgtttc tgtttctgaa atcatctttt
      181 taaaaaatgc aatccggatt ttttcaaaat gccatgaatg ataataaatt tgtataagca
      241 ataatttatc atgagcacca caagaattat actgattgtt ggattactgt tacttgccgg
      301 tttcagtatc tttcagaagc atgtcaggca taagcaggga gagaagggaa acggacaagg
      361 gaaaggcagc ggaaaatcat cttcttccga agccataaca gatgattatg agccttattc
      421 aggaaagtaa catgttttaa agcctgtttt ttgcggcaag atgcctgaag ggattgacca
      481 ctttctgcag gagtctcagg ctgtcggtca ttatttcggc ggtcccctgc atgttctggg
      541 taaattcaag ttcccggcca taaagagtct tcagtccttc aggcaggatt atctctatta
      601 tgtaggcatc tccggccggg acaagtgatt tggactccac aatcccgcgt accattccgt
      661 attcaaggta gggatagccg gcaagcttga tgtttactgc ctgacccgtc ctgactttcc
      721 ccgatctgtg cattttaagg tccattcttc cgatatagtt acccgggaca aggggaacaa
      781 cggttaagac aggttcatcc ctggccacca tctgattctg cttccagaac cttgtaaaag
      841 ttacaatacc gtcaatggga gaaatgataa gataagtatt ttcccatatt cttatctgtg
      901 ccctgagatt attgagggat tctgtaaggg ctgccagtaa tctttcccgt tcttctgtcc
      961 tttttatcct gtaatcctgc aactcctgat tcttctgtgc cagttcaagg gttttggctg
     1021 catgatcaag cctgacgtcc tgcagttcaa tattgatttt gttgaaggtc tggcgtgaaa
     1081 tatcaaattc gctttcggaa aacaccgctc ccgcaaaaag tgatgaatcc ctggtaaact
     1141 tttttgcttc aatacgctga ttttccgaat aaaacttctc cttttccttt actctcctga
     1201 tgtattcctc cagtactgcc gtttccctga taaccgacga tatcttatat ccgtagaaat
     1261 catttttaac atagttttca taatcggaca ggtttttcag aaaagcaccc cagtattcct
     1321 gtatttcccc gagttcagaa aatgacggaa gggatccggc tgatactgat ccgggatctt
     1381 cgatttcccc ggtgcattct ttcagtttcc tcacctggtc aattgatgca gctgtttcca
     1441 tcacggcgag gagccgtcct tcacggacgg tctcaccatc gctgacgtac cagttcagaa
     1501 tccgtccgct tattttggac acaagggtaa ccggagggtt ttccgtggtg atctgaacag
     1561 gcgaaggcac cacattcgga tagcggataa tccacgcaaa aaaaaggata atcagaatta
     1621 ccgcagagat tgcagctgtt ccccatctgg cgagctttcc gggcggatct gaaataattt
     1681 cccggaccgg ttcggaatgc agtatttccg ttttattcat tttcattttg tcaaatcagt
     1741 tgccaagctc aagctggttt ttaataaggt tgaaataagt tcccttctgt tcaataagcg
     1801 aattgtgatt tcctgtttcc acaatccttc ccttgtccat aacaacaatt ttatcggcat
     1861 tccgcacagt gctcagcctg tgtgcaacga ttaccacagt ttttctgctg aagtacccgg
     1921 caaggttttc cacaatggct ttttcattat tggcatcaag agagttggtt gcctcatcga
     1981 atatcaggat atccggatcc ttatacagaa cgcgggcaat aagaattctc tgcctctgcc
     2041 cttcactcag gccatgtccg tttgccccta ttttcgtatt gtagcccaat ggcagtgatt
     2101 caatgaattc cctgatattt gccatttcaa ctgccctgaa aagcctttca tcatcaattt
     2161 cagaatttcc cggggaaata tttgatgcga tggtttcagg aaaaatgaaa ccatcctgca
     2221 ttaccgcccc tgttttttcc ctccaaagcc tcaggctaag attcttaagc ctggtgtcac
     2281 ccaccaggat ctcccccatt accggctggt aaaatccaag tatcagcttg agaagggttg
     2341 ttttgccgct gccactggtt cccacaacgg ctgtggtttt attttcttcg attatcaggt
     2401 cgatatcctt taaaacaaag ggtgaatgcg gtccctcata ctgaaatgac agattactga
     2461 ttattagatc tttcttttca ggaagccgca taatctttga atccggatct gcttcctctt
     2521 cttccattcc gtgaacttcg gcaagcctgt tcaggctcat gcccgcatcc tgtgtcgtgc
     2581 ggaagaaacc gataatctga ttcaggggca cattgagctg gccaatgata aactggacag
     2641 ccagcatcat accaagagtc atatcacctt tcagaaccgc agttgcagaa atgactgtga
     2701 tcagcagatt tgttatctca ttgagaaaag tgcctcctgc agtctgatac tgaagaagac
     2761 ccagtccttt tgatttaagg ctgaacagag aagcctgcag gttttcccag tcccagcgtt
     2821 tcagggattc actctgggta agctttatct cctgcatccc gttcacaata ttaatgagtt
     2881 tgctgtttgc aactgacatt tctcggaatc gctcatgatc aagcctggct cttgatttca
     2941 taaatgcaga tacccagagc acgtacaata ccgatcctgc caggaaaagt gcaagtattt
     3001 tcaggctgta aacagcaaga acgatactga agacaatgat gttgaaaaag gagaacatga
     3061 tgctcaggct tgttgaagta agaaaatttt caatcctgtt gttgtcatcg atcctctgaa
     3121 gaatatctcc tgtaagcttg gtatcaaaga atgctatggg cagggacatc agtttccgga
     3181 gaaaaccgga aattattgaa acatttaccc gggctcctat gtgaagcagc agccagcccc
     3241 tgatgaattc aaccgacata cgtccgatta caagagccag ttgcgcgaac agtatcagat
     3301 agataaatcc caggtcgttg ttattgagcc cggtatcaat tattgcctgc gtgaggaagg
     3361 gaataataag ctgaatcagg ctgccgacaa gcagtcccag catcagctgg ccgaaatatt
     3421 ttttatacag cttaaaatac cttgcaagga acctgaagcc atgaacgggt tcaggctcat
     3481 tttctttctc cagaagtgca ggcgtaggct ccagtatcag taccagtccg gcaggcttgc
     3541 catcttccac tgtacttgcc cattttttta caaattcatc cctgctgagg gaaagaaggc
     3601 ctacagccgg atcagctatc ataatcttgt tcttccggat cctgtaaacg acaacaaagt
     3661 gtttctgcct ccagtgcact atgcagggca gcggtacctt ttcaagcaac agatcgaagg
     3721 ggatctttac tccgatagtt ctgaatccca gactgtttgc tgcttctgac aaaccaagaa
     3781 aagaaactcc ttcccgggtt atgtaacatt ttcgccgtat ggattccaga ttgaagtttt
     3841 tcttgtaata agatgctacc attttaaggc aggcaggacc acaatccatt gcatctggct
     3901 gtcttacaaa aggaaatgac attcttgccg gaaaaagata ttatcggttt aaattattta
     3961 atatttaccc tgaaagagat ccggtaaatt attgtgcaat gtaattattt taataatttt
     4021 tttacgcaaa ctaaatagta tatttgtttc tcagctcaga ttgaccagaa ttgtttaaga
     4081 aaataacaga ttcaaagatc attgcaggca ttaaggctca ggatgataat attcttgagt
     4141 ggatttatga taattattat cagactgtag aaaatcacat aataaaaaac agcggttcac
     4201 ctgatgatgt tcctgatgtg tttcaggata ccatcattat tctgtacaga cagattactg
     4261 ctgattccct tgaattgtcc tccgatctga aagcctactt ttttggaata gcacgcaatc
     4321 tctggaatgc cagtctcagg cagaagaaca ggatgaccgg aatttcaccc cgttttgaca
     4381 tggcagatga atcagacgca gaagaaaatg aagatctgat atttgaaagg ataatgagca
     4441 gggcattcta taaactgaaa ccggattgtc agaccatact gacattatat tccgacggtt
     4501 acagctacca ggagattact gagaaaatgc atctgaagag tgaggtatat gcacggagaa
     4561 agaaatacct gtgcaaggaa gcacttctcg acctggtaag ggcggattcc gaatatcagg
     4621 aatacttgcg tttccgtaag taataaacca atgcggctgc gggcaatgcc agcacgaaaa
     4681 ggattacagc cattaacctg acaggtgggt atatgggtga attatccccg tacactatca
     4741 gtgtggccca gaaatatgga tgagatttga gctgggtggc atttctcaga taatcagccc
     4801 ttgattttct cagggagttg ctttttgtat ggcctttttt aagattttca tagaacctgc
     4861 ttactatttc cgttcctgat ttatcctcaa tttcccacat tgacattacc actgagcggc
     4921 ttccggagta aagaaatccc ctggccaggc tgagaatgcc ttcacctgat gacaaaatac
     4981 cggttcccgt attgcaggaa ctcagtacca ccattttagc cctgagcggt attccgtaaa
     5041 cttccgaagt atagagcagg ccatcttccg gatcgtcttt catctgcgaa aatatcattg
     5101 ccgaattcat tggatgctga tcatttagca gggtatgcat tgccagatga acaatattga
     5161 agttcagggc ttccttttta aaaaccgatt cccgtgcctt ttcattcaga aaaagattgc
     5221 cccggaaaat ggaagcaaca tattcagctt cctggcgggc atagggcaga tcatacagaa
     5281 aaccccttcc ctgctggttt tgcgagaaca gtgaatcgag atttatatct gttccgtaaa
     5341 agggtgcaaa agctgccagc ctgttcctgc ctgaaatgct tctggtaccg gtctccttca
     5401 ttaatgttgc cgaataggca tatgaaatgt caaaattatt catcagataa tcaagcttcc
     5461 tgtaaagaat gccttcaccc tcatattttg aggccagtat cgtttcgaat ggaaggtatg
     5521 acagatagtt gtcgggagat atcagcagat tccgggatat aaaatacttt cgcaccggct
     5581 caatcagcat tttgtaaagg tcggtaccgg tttcctggta attgacaaaa gcactgcggg
     5641 catccttgac gagagccgga tttgaaagca gttccctgaa tttcccgaga gtcttgaaca
     5701 atatggaatc gattctgcat gaaaccagtt ccctgtgttt tttgttgaca acaaagatgt
     5761 aaagcaggga atctgaaaca atgtaactga tatagttgat tttttttccg actattgccg
     5821 gtatttcagt cattaaaggg gcgacagtat tgtatttgag tgcaaaatac tcgggatagt
     5881 atttctcaaa ggtaagtatc agcgagtcgc gtgcccggat agctaccagt atcttttcat
     5941 tccattcggc aagaaactgt ttgtttggtt ttacttttcc tttttccagg gagatcttcg
     6001 aattaagcat tcctattttc cgctgaagag actgttcata atcagctatt tcaggaggga
     6061 tatgcagctg tatggcattc agttcacggg ttgttgccag caggcctgcc accttgcttt
     6121 tttctgcaat ctgaaaggtt ttttcaagat aggaagggtc tgagctcagt ctgtaacaaa
     6181 gctcggaatc ccttatagcc atcagatagg attttctgta atggtcaccc agaacaatcc
     6241 tgctttcctc ctcgctgatg ttcagcctga tcttgtcaat agcctcaatg atataacggg
     6301 aagtggcagc tgccgccctg agtatgccaa tatcctgtgt ttcggcaaaa atatccgtca
     6361 gaatctcata ctttgaacgg agtatcctta tatatcttct gtcggactgt acagaatcaa
     6421 tttccggatt cgcgagtgaa tcgaatgatt gttttccctg cgtgccataa aaaagaagtt
     6481 cctggatttt tgcaagtgct tccctgctcc ggccgttgta atgcagggcc ctggcatatc
     6541 cctcatagac aggatctctc agattggcat cctggggatg ccgttcaaga tagtcattac
     6601 agacggaaaa gtattttatt gcagcctcag catcattcct gtagttaagc agaaaagtgg
     6661 catagttctt taatgcttct atataatatc ttgagtccgt gccataatag ctgcccgatc
     6721 tttccagcaa ttccgtcaga agcgattcac ctcttctgac attgccggca tttgcctggg
     6781 caagggcata ggtatggatc agattgaatg aatgaaagga attatccttt accgccagct
     6841 ctatgccctt tctgaaatat tcgtcctcct tatcggtcat gccgagatat ccgtaattta
     6901 tggccatgct gttgatcagt gtaatataat tctgatccat ttccatatga ttcttttccc
     6961 acaccgattc tgaatgttca aaatagatcc tggcttttgc gcaatctccc attctcgcgt
     7021 atcctgctcc gatactgctg tataaatcgc agaggtccct ctgctgaaaa gctttcagta
     7081 ttcccagtgc cctgtcagtg tattcaatga aattcttata gtcattattc agaatcgaag
     7141 ccccgataag tgttgcatag gctgaagcga cttccgggct ttcctcacca tagtatttgc
     7201 cggacatttc tatgtaccgg gtcagatatc ttctgaccat tgaatagttt cccagataat
     7261 tatgtgccac gccaagattg aaaagagcct taagatactg atcgtctgca atattcagac
     7321 tgtctttcag ccggactgat tccagcaggt gggaagtagc attttcatac cgggatgaca
     7381 gcaaattaca cacgccggca taatagaaaa gctctgcctg tacaatctga tcagaatggt
     7441 tccccttgat taacttttcc aggctgtctg ccagtgacaa caggcccgca atatcttttc
     7501 ctgaactgaa atattgtatt ctgtggctga ttctgtcgag ggagccggaa cctgtgttgc
     7561 tcagatgttc gataagggga agaatcccgg gttccgagaa agcatccggg gaactgaggg
     7621 aatattttgc cgcgctgaaa tagaggattc ctgcaagaat tagaaatctg acctttgaat
     7681 agtgtttaac tgactttcgg ggcactttca atcctggttt ataattattg acttcgattt
     7741 tcaaaaatac cttctatttt tgaaccatta acaaaaaacg agcataaacc tgaatttcta
     7801 aaacactgta tccgtgtgtt ttaaaaaatt ctgcaatttt ttttcacaat ttaatgtcac
     7861 aaaaagggtt caaatgacat gtatttgcag aatgaacaat aattaatttc tgaaaaaatc
     7921 aagtaacaaa taaaacaaaa aaaaaaatga aaaattctaa ttcttccaca accagagaaa
     7981 tttttgcagg ttatgaactc acaattgatg aaatgttcaa tataagaggt ggggacaaag
     8041 ttaaaggtga accaataatt ttacctccat cccctcctat caagatctga gattaaaaaa
     8101 taaatatata cctgctggca ggtacaggtt tttttatatt gttgttttaa ttttccggca
     8161 agcgggataa acaattcctg cccgaggcag ggtatgtgta tatacatatt aaggcagcgc
     8221 tataaaaatt ctctggcatg ttgattgatg agttttttaa agaatttgaa atataaatat
     8281 atatccatga aaactattga tttttcttac ttcattgagc ggtttcttgc gggagaaatg
     8341 aatgaagccg aagaagagtg gtttcgcaat gagcttggca ggaatgaaaa actgcgtgac
     8401 gaaattgctc tgaggcagct cacaaacaca acccttaaga atcatgatgt tttgcagctc
     8461 agaaacaaac tgacggcaat tgcatcaaga aaagctgaac aaacaccatc caaaaaagga
     8521 cacaggcaca aggtccttac cagggcagcg gtatttgcgg gtctggtact ctttggcacg
     8581 cttgcattcc ttaacaacag gacaatgacc acggatgagg tctttgatca ttactacaag
     8641 ttttatgaca ttacgactcc cctgcgatct gcggataatg ccggcaatac tgattatctt
     8701 acggcaattg aatatttcaa tgtaaaggat tatcacaatg cagcgtatta tttcagcagg
     8761 gtcctcagca gcgatcccag gtatattgag tcggttctga tgacgggagt atcgaaattc
     8821 gaagaagaaa aatattccga ggcaaaatca tcctttacaa aagttatgga cgacagggat
     8881 aatcttttct ttgaagatgc ccaatggtac ctggcattgt gttttctgaa gacatccgaa
     8941 aaggacaagg ctgtaagtac gttgaaagcc ataaaggaat ccggcagtgt gtacagcagg
     9001 aatgccgtaa aaatcctgaa aaagataaaa tggtaaacag cacggcgggc ccttcataag
     9061 ggtggctcac cgtttttcat tcttcctggc aattattttt acctcggtcc gccggttcat
     9121 cctccttcct tccgatgtaa tattgtcacc gattggtgag gtgtttccat agcccttgta
     9181 ctgaagcctt ccgggagaga tgccgctttc aacaagatag ttgacaactg acaatgccct
     9241 tttttcagat aactgcagat tgtgctggtc ggtaccggta gaatcagtgt atcctcctat
     9301 ttcaacaaca aaatcctcat tgattctcag taattcggcc agatttgcaa gttctgacat
     9361 tgattccctc ttcagttccc agcggtcaat ttcataaaat acattggcaa gaagcatttt
     9421 ctctcctatc ttcacagggc tcagctctat tttttttatc aggggttcca ttacggaatg
     9481 ggttccctca aacataaaat tctccgaata gaaaagatat cccggctttg atacatttat
     9541 tccgtaattg tttcccgaag gaaggcatat tagaaaattt cccagatcgt ctgtcctgct
     9601 tttaatggtg actctgtttg ttgtaaggtt tatcagttca aattcggctt tcagcatttt
     9661 tccggtctcc cggtcagtaa ccctgccttt gagataggaa acgggattcg gtctgaatga
     9721 tttatcaagc gtgaaccaga atatatcttt cccgcttgaa ttatcccttt tggaagaaaa
     9781 atatgctttc cggccgcctg cctctatcac aagccccatt tcatcaccag ccgtatttac
     9841 aggataaccc aggttctgtg gatcagtcca tgtgctgtca tccttcattc ttgtcaggta
     9901 gatatcaaaa ccgcccatcc ccggtctgcc attggatgca aaatagaggg ttctgccgtc
     9961 aaaatgaata aagggcgaca tttcatcgcc ttcggtattg attttatctc ccatattaac
    10021 tggttccttc caggttccgc tttcattcat tactgaatac cacagatcct ttccgcccat
    10081 cccgccaggc ctgttgctcg aaaagaagag catgttcccg tctgcactga ttgagggcgt
    10141 tgattcccag aaagatgtat tcactggcgg gccaaggtta taggggaagg accatttgcc
    10201 gttattattg gcagaaaaat aaatgtcaca gctgccatat ccgcccggtc tgtcacaagc
    10261 agcgaaatac atatagtttc cgctggaaga aagggttgca gcgccttcgt tttgtgcggt
    10321 attcaatggc gctccagcat taacggggct cgtccagccg gtatccgaca ggtaactaat
    10381 ataaaaatct tcctggtaat tcgggcggtt gagaaaatcg gtggaagtgt aggattgtct
    10441 tg
//
LOCUS       DAOSPX010000077        10342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6292_length_10342_cov_4.546904, whole genome shotgun sequence.
ACCESSION   DAOSPX010000077 DAOSPX010000000
VERSION     DAOSPX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6292_length_10342_cov_4.546904"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLZ75_05475"
     CDS             <1..558
                     /locus_tag="PLZ75_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71882.1"
                     /translation="DEEISVSRIAIPGKYTTANLLFSLAWPDAVNKTEYLFSGIVDAV
                     LDGRADAGLIIHETRFTYQEKGLIKLADMGQFWEELTGLPIPLGLIAVHRRIPFEIAQ
                     KVNRVIRRSLEYAGRRSDAISGFIEENAREMEKSVIESHIRLYVNEFTAGLGQTGRKA
                     ICTLYGKGREKGVVPEMPEKIFLEA"
     gene            741..1319
                     /locus_tag="PLZ75_05480"
     CDS             741..1319
                     /locus_tag="PLZ75_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOS71883.1"
                     /translation="MKFEDLVFRTRSFRRFDESVRIGRDILEKLAGLARLSASAANRQ
                     PLKFILISSPDECSKVFPSLAWAGYLKDWEGPAPGERPSAYIIILGDRSIAETFGVDH
                     GIAAQSIMLGATEAGFGGCMIASVKKELLRKNLNIPERFEILLVLALGKPVENVIIDE
                     IKNGDVKYWRDLQNNHHVPKRPLNELIIRFVH"
     gene            1327..2043
                     /locus_tag="PLZ75_05485"
     CDS             1327..2043
                     /locus_tag="PLZ75_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71884.1"
                     /translation="MKRYLSVLLHILMLIHVLTGQEAKQGIQAMLFRGIVMSAETKMP
                     LSGTQIMINRAFASISDTEGKFAFYVNRLDTVKFSMLGYKSTILHISDTLQGYEFIAG
                     IYMNTDTLSIDEIVIMPGLRTLKSDLLRPAGKPDPELENARYNLEVSAYQGKITQSKL
                     GDPDMNYKYLQQKYRAEVYSKGQIPSDRIIGLSPFMVVPAVYLLMNGFPGKPPSIKPH
                     LSDYELNMIYKKYLETYGKK"
     gene            complement(2155..3066)
                     /locus_tag="PLZ75_05490"
     CDS             complement(2155..3066)
                     /locus_tag="PLZ75_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOS71885.1"
                     /translation="MFNKLIASMLPYLPKKLVWIFSKSYISGESVGDAIRVSKELNAR
                     NIKVTIDVLGEYIESLHEAETNKLEYLNLIDTAHRHGIDGNFSAKPTSFGLLIDEEAC
                     FSHMREIVARAASYNGFVRIDMEDSPCTTKEIELFRKLKKEYPANVGIVLQAYLKRTM
                     DDLKSLSDLNSGETPLSIRLCKGIYIEPEAISYKKYDEINRHFTEDLEYMFKNRIHVG
                     IATHDKILIENAYRLISEYSVPEHLYEFQMLYGVCPNLRQSIVDKGHTMRVYVPFGEK
                     WFGYSTRRLKENPKMASHIIRAIFIKG"
     gene            3249..3815
                     /locus_tag="PLZ75_05495"
     CDS             3249..3815
                     /locus_tag="PLZ75_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS71886.1"
                     /translation="MMSDQEIISGCVRHERRAQKMLYDKYSGFLLGICLRYASDRAEA
                     EDILQDSLLKIYFGIDDFTGSGSFTGWLRKIAVNTAITHYHRNLKFRYSVDIEEYVST
                     ETGVSSFEEDFFTSDELFRVLNELPAGYRMVFNLYAIEGYKHREIAKILKIDTNTSKS
                     QYCRAKAVIRNKLEKLRTLKANYPAGGR"
     gene            3834..5174
                     /locus_tag="PLZ75_05500"
     CDS             3834..5174
                     /locus_tag="PLZ75_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOS71887.1"
                     /translation="MKRKKEDKMLRDLFRQKLEKAEVHSSPSFEAEFFRKLGRKEFLR
                     FDPLRFNAWYAGVIITAAALGLIFYLNGSSDNEADAVLPESGRPAAGLIRETEESLPP
                     RINPWETEQEGKDISEDSAVEEPPGSQRYGRLGSDVDGGQAPLIPPGTEIKNAINGQN
                     IFQKTDGGSERLRSPLQHSDLIMVSQERGCSPLKVSFRARSGDFNRYRWTFGDGGVSE
                     EAGPDWIFDMEGEYTVTLHAYKSDELQAVGSRVIRVFPRPLARFEIIPENGELSENRI
                     RLINYSVNAVRFTWDFGDGHISGLYEPVHVYREGRDHSVKLTAFSEEGCTDTIIVSRL
                     PDKPENYIEFPNAFLPGQNGPTGGFYSASSDDAATVFHPVSSGVTNYQLKIFSKLGLL
                     IFESNDINIGWDGYYKGRLCQAGVYIWKVRGTYINGETFTRMGDLTLLKNTDRM"
     gene            5327..6316
                     /locus_tag="PLZ75_05505"
     CDS             5327..6316
                     /locus_tag="PLZ75_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOS71888.1"
                     /translation="MHKRISYIVILSFVLFVKSQSQDPTFSQFYANPLYLAPSFAGAT
                     EDYRFGMNYRNQWPAVPGVFHTYSISFDKFLHNFNSGIGVLATYDVAGSGDLSTTNIG
                     LLYSYDFHISQDWHIRPGVEFKFYYLGLNIYKLVFNSQLTGSGTTPSVYPPPFENVAD
                     VDFATSALVYNDRIWAGFTLDHLLVPKTSFYGEEGHVPVKFNLFGGTQILRKTRLKVK
                     LQEVLSVAFNFQKQAKFYQTDAGVYYYRDPLIFGLWYRGIPFVTSQAGDAIIGLLGVK
                     TGPFHLGYSYDFTISNLISSTAGAHELSLIYEFTSFSINSPKRKMRAIPCPEF"
     gene            6406..7011
                     /locus_tag="PLZ75_05510"
     CDS             6406..7011
                     /locus_tag="PLZ75_05510"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOS71889.1"
                     /translation="MKNLSIALFSVLFLAITVLYILHFTGRKKETVRESVSSPVEVRS
                     EGIAFVNIDSVIMNFDMFFDRRNELMEKQRKAEAELTSKGSRYERDARDFQEKVTKGL
                     VTRATAAEMEKSLLQQQQDLITLRDDLQTNLMEEEQVMNRQIIDYITTCLEQNKSEFN
                     YQYVLGKSFGSVVLYGDEALDITRRVSEAINSKYRSEKSKK"
     gene            7017..8282
                     /locus_tag="PLZ75_05515"
     CDS             7017..8282
                     /locus_tag="PLZ75_05515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOS71890.1"
                     /translation="MGFATRWLSEKALFPELFRDKPCDNTGIIVVIPAYDEPDIATVL
                     DSLASCSRPGCSAEVIIIVNLRKDAGRQAILNHRKSIENIEDWKKKNASCFFRLFHYD
                     AGSPAVKNWGVGLARKTGMDEALRRFDAAGNPDGIIVCLDADCTVKENYFTAIEKEFL
                     NRRKAGACSLYFEHPVEDDPASVSAFYGIILYELHLRYYVGALRAVQYPYAFHTVGSA
                     MAVRAREYMRAGGMNRREAGEDFYFIQKLVPSGRYFSLNSTTVYPSPRQSHRVPFGTG
                     ATMGKLAGDAEQQYYTYNLDAFRELQWLFSLKEDLFSSGKREAERMYDKFPPGLRYFT
                     DRDEWLVKLEEIRSNTAGYESFCKRFFGWFNMFRIVRFMNSVHDGIHERRPVVRAACD
                     LLRHTGHDLIPDDALQLLKYYRQLDRESQ"
     gene            complement(8276..8614)
                     /locus_tag="PLZ75_05520"
     CDS             complement(8276..8614)
                     /locus_tag="PLZ75_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOS71891.1"
                     /translation="MARFSLPDRIPAFLRNKYVLTILLFLIWILFLDSNNLISRYREM
                     RELKKLRNQRDYYINRIEEESRKLRELKTGNDNLEKFAREQYRMKKPDEDLYIVLTPR
                     EDRKNSKQNH"
     gene            complement(8713..10002)
                     /locus_tag="PLZ75_05525"
     CDS             complement(8713..10002)
                     /locus_tag="PLZ75_05525"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOS71892.1"
                     /translation="MKRILIFIIFSAFLQPVIRGRAYDPDFYTDSIFDKRIKTVQLFR
                     EEWNLSYPLIRLNSPDRLAFHFDLLDEHPETYYYTLIHCNKDWQRSELFTGDYLEGFT
                     YDEISKYRPSFNTTVSYFHYSLIFPEERLKPMLSGNYILYVYSGDDPDRPVITRRFMI
                     TEDAAGIDISVHLPRTEDSRNEKQQVDFSINIAGLGIIDPFRNIYAFILQNGRWNTSR
                     KNLKPDVYGSNELKYTSLSDRNIFRGGNEYRFFDIKSIKRPGVNVERIDFRAPDYHVL
                     LLPSDNREFKPYFYSQDINGKYIIARQEGRDPDTDADYLYVYFTLPSYEKPDAGELYV
                     SGLLADWTFGGDNRMLYNPGMKQYECTMLLKQGWYNYEYVYISRDVKDGTSSRFEGSH
                     SETENDYHILVYYRNPRDRYDRLLASGSINTLNRMTH"
BASE COUNT         2781 a         2212 c         2486 g         2863 t
ORIGIN      
        1 gatgaggaaa tatcagtctc acgcatagca attccgggca agtataccac ggccaatctt
       61 ctcttcagtc ttgcatggcc tgatgcggtc aataaaaccg aatatctctt ttccggcatt
      121 gttgatgctg tgctggacgg aagagctgat gccggactga taattcatga aaccaggttt
      181 acctatcagg aaaagggact gataaaactg gctgatatgg ggcagttctg ggaagagctg
      241 acaggcctgc ccattccgct gggcctgata gcggttcaca ggagaattcc ctttgaaatt
      301 gcacaaaaag taaaccgggt tatcagaaga agtctggaat atgccggcag gcgttcagat
      361 gcaatcagcg gattcattga agagaatgcc cgggaaatgg aaaaatcagt gattgaaagt
      421 catataagat tatatgtgaa tgaattcact gccggtctgg gccagaccgg caggaaggca
      481 atatgcacac tgtatggaaa aggacgtgag aagggtgttg ttccggaaat gccggaaaag
      541 atatttctag aagcctgaaa aggaatgttt ccgtagttca gagcaggaat gtttccggaa
      601 tccagaagag gattgtttcc ggaagcctga acgggcatgt ttccggaatc cagaaccaga
      661 atatttcctg gtgaatggat gccccggttt attttcttaa ttttgctttt aaatttcaga
      721 aattaatatt tttccttaac atgaaatttg aagatctcgt attcaggacc cgttctttca
      781 ggcgttttga cgaatccgtc agaataggcc gtgatatcct tgagaaactg gccggactgg
      841 caaggctttc ggcatccgcg gccaacaggc agcctctgaa gttcattctc atcagttcgc
      901 cggatgaatg ttcaaaggta tttccatccc tggcatgggc tggttacctg aaggattggg
      961 aaggtcccgc accgggcgaa aggccttcgg cgtatattat cattctgggc gacaggtcaa
     1021 ttgccgaaac attcggagtg gatcacggca ttgctgcgca gagcattatg cttggagcta
     1081 ccgaggccgg ctttgggggt tgtatgatag catcagtaaa aaaagagctt ctcagaaaga
     1141 atctgaacat tccggaacga tttgaaatat tgcttgtact ggctctgggc aaacctgtgg
     1201 aaaatgtaat cattgatgag attaagaacg gagatgtgaa gtactggagg gatctgcaaa
     1261 ataaccatca tgttccgaaa aggcccttga acgagttgat aatcaggttt gttcattaaa
     1321 tctggtatga aaaggtattt atctgtttta ttgcatattc tgatgcttat ccatgttttg
     1381 accggacagg aggcaaagca ggggatacag gctatgcttt ttcgcggtat tgtaatgagt
     1441 gcagaaacaa aaatgcccct ttcaggaaca cagataatga ttaacagggc ttttgcatct
     1501 ataagtgaca ctgaaggtaa atttgcattt tatgttaaca gactggatac cgtcaaattc
     1561 agtatgctgg gttataaatc cacgatcctg catataagtg atacacttca gggttatgaa
     1621 ttcattgcag ggatctatat gaataccgat actctgtcga tcgatgaaat agtgataatg
     1681 cccggattaa gaaccctgaa atcggatctg ctgagaccag ccggtaaacc agatcctgaa
     1741 ttggaaaatg caaggtacaa tctggaagta tcagcatatc agggaaagat cacccagagt
     1801 aaactagggg acccggatat gaattataaa tatctgcaac agaagtaccg ggcagaagtt
     1861 tattcaaaag gtcagatccc ttccgacagg ataatcggat taagtccttt catggtcgtt
     1921 cctgcagttt atcttctcat gaatggattc cccggaaaac cgccctcaat taaaccacac
     1981 ctctctgact atgaactgaa catgatttat aaaaaatacc tggaaaccta cggaaaaaaa
     2041 taaatctgct ccccgctccc tattccccac accacactcc acactccccg ctcattattc
     2101 atcactcaac ttccgcttcg cgaaactgcg tcgttttaca ccttgtctca tctctcaccc
     2161 ctttatgaag atggccctga taatatgact agccatcttt ggattttctt tcagccgccg
     2221 ggttgaatag ccaaaccatt tttccccgaa aggcacatac actctcatgg tatgaccctt
     2281 gtccacaatg ctttgtctca ggttcgggca tacgccgtag agcatctgga actcatacag
     2341 gtgttccggg acgctgtatt ccgatataag ccggtatgca ttctcaatca ggattttatc
     2401 atgtgtggca attccgacat gtatcctgtt tttgaacatg tattccagat cttcggtaaa
     2461 atgcctgttt atctcatcat atttcttata tgaaatggct tcgggctcaa tgtatatgcc
     2521 tttgcacagc ctgatgctca ggggtgtttc tccggaattg agatcgctca ggcttttcag
     2581 gtcatccatt gtccttttca ggtaggcctg cagaacgatt ccgacatttg caggatattc
     2641 ctttttcagc ttcctgaaaa gttcaatttc cttcgtggta cacggtgaat cttccatgtc
     2701 aatcctgaca aacccgttgt atgatgctgc ccttgcaact atttccctca tgtggctgaa
     2761 gcatgcctct tcatcaataa gcagtccgaa acttgtgggc ttggcagaga aattcccgtc
     2821 tatgccatgg cggtgggcag tatcaatgag gttaagatac tctagcttat tggtttcggc
     2881 ttcgtgaagg ctttcaatgt attcacccag aacgtctata gtgaccttta tgtttctggc
     2941 gttaagctct tttgatactc tgattgcatc accgaccgat tctcccgaaa tataggattt
     3001 tgagaatatc cataccagtt ttttcggaag gtatggcagc attgatgcta tgagtttatt
     3061 gaacatggcc ggacaaatgt tgataattgt taatttctcg ctgctaatta agcttgtatc
     3121 catttaattt ttgatgacct ttgtcatcgg aaactgatac aaaggaaagt caatttttca
     3181 atatttatta tcggaaagtc aatgcaacca tttttccttt tagcttgtct taaacttgtc
     3241 ctctgcttat gatgagcgac caggagataa tcagcggctg tgtcaggcat gagcgcaggg
     3301 cgcagaaaat gctctatgac aagtattccg gtttcctgct gggtatctgt ctgagatatg
     3361 cttccgacag ggcagaggca gaggatatac ttcaggacag tctgctgaag atatacttcg
     3421 gcatagatga ttttaccgga tcaggttctt ttacaggctg gctgagaaaa attgcggtta
     3481 atactgccat cactcattat cacaggaatc tgaaattcag atatagtgtt gatattgagg
     3541 agtatgtttc gacagaaacc ggtgtttcga gctttgagga ggattttttt acatccgatg
     3601 agcttttcag ggttctcaat gagcttcccg ccggttacag aatggttttc aatttgtatg
     3661 caatcgaagg ttataagcac agggaaatag caaaaatact gaagattgat acaaatactt
     3721 caaaatcgca gtattgcagg gcaaaagcag tcataaggaa caaactggag aagttaagga
     3781 cattgaaagc taattatccg gctggcggcc gttgaaaatt tggttgtaat caggtgaaga
     3841 gaaaaaagga agacaagatg ctcagggatc ttttcaggca gaagcttgaa aaggctgaag
     3901 ttcattcctc cccgtctttc gaagctgaat tcttccggaa actgggcaga aaggaatttc
     3961 ttcgctttga tcctttacgc ttcaatgcct ggtatgccgg tgtgatcata acagccgcag
     4021 ccctcggcct gattttttac ctgaacggca gcagtgacaa tgaagcagat gcagtgttgc
     4081 cggaaagcgg caggccggcc gcaggactga tacgggagac ggaagagtca ttgcccccgc
     4141 gcatcaatcc gtgggagacg gaacaggaag gaaaggacat ttctgaagat tccgccgtgg
     4201 aagaacctcc cggaagtcaa cgttacggca gactgggcag tgatgttgat ggcgggcagg
     4261 ctcccctcat ccctccaggc actgaaataa aaaatgccat aaacggacaa aatatttttc
     4321 agaaaacaga tggcggaagt gaaaggctga gaagtcccct gcagcattct gatctgatta
     4381 tggtatcgca ggagaggggc tgcagccccc tgaaagtatc tttccgggca cggtccgggg
     4441 attttaacag atacagatgg actttcggtg acggcggagt atctgaagag gccggtcccg
     4501 actggatttt cgatatggag ggtgaataca ccgtaacact gcatgcctat aagtcagatg
     4561 agcttcaggc tgtcggatca cgtgtaataa gagtattccc caggccactt gcacgctttg
     4621 aaataattcc tgaaaacggg gagttgtctg aaaacaggat aaggctgatc aattattcag
     4681 taaatgcggt caggttcaca tgggatttcg gagacggcca tatttcaggc ctgtatgaac
     4741 cggttcatgt ttaccgcgaa ggccgcgatc acagcgttaa actgacggca ttttcggaag
     4801 agggctgtac tgatacaatc attgtgtcaa ggctgcctga caaaccggag aattatattg
     4861 aatttcccaa tgccttcctg cccggacaga atggtcccac cggggggttc tattcagcct
     4921 ccagtgatga tgccgctacc gtctttcatc ctgtttcctc aggagtgact aactatcagc
     4981 tgaaaatatt cagtaagctc gggctgctta tttttgagag caatgatata aatataggct
     5041 gggacggtta ttacaagggc cgcctgtgtc aggccggggt atacatctgg aaagtgcgcg
     5101 ggacatacat taacggggag acctttacca ggatggggga tctgacactt ctgaaaaaca
     5161 ctgacaggat gtgaccggta tttccggccg tttcagacag atccgccata cgtcaaaaaa
     5221 aattctgttg ataactttaa cttttggtga taaattccgt acaacggaga aatcttgcat
     5281 taagagattt agttaattta gcaattggaa agttaagttt acaggtttgc ataaaaggat
     5341 cagttatata gttatactgt cattcgtcct ttttgttaag agtcagagtc aggatccgac
     5401 attttcgcag ttttatgcga atcccctgta tctggctccg tcatttgccg gagctaccga
     5461 ggattaccgc tttgggatga attacagaaa ccagtggcct gctgtgccgg gtgtttttca
     5521 cacctacagc atttcatttg ataagtttct tcacaatttc aattcgggga taggtgtgct
     5581 ggcaacctat gatgtggccg gatccggtga cctgagcacc acaaacatag ggttattgta
     5641 ttcctatgat ttccatatca gccaggactg gcatatcagg ccgggtgtgg aattcaagtt
     5701 ctattacctc ggactcaata tctacaagct ggtgttcaac agccagctga ccggaagcgg
     5761 aacaactcct tcggtttatc ctcccccttt cgaaaatgtt gctgatgtgg attttgccac
     5821 ttcggcactg gtttacaatg acaggatctg ggcaggcttt acacttgacc acctgctggt
     5881 gcccaagact tccttttacg gcgaagaggg gcatgtacct gtaaaattca atcttttcgg
     5941 gggtactcag atactgagga agacccggct gaaggtcaaa ctccaggagg tgctttccgt
     6001 tgctttcaat ttccagaaac aggcaaaatt ttaccagacc gatgcaggtg tttattacta
     6061 ccgcgatcct cttattttcg gcctgtggta ccggggaatc ccctttgtca caagtcaggc
     6121 cggcgatgcc attatcgggc ttctcggggt aaagaccggt ccgttccatc tggggtacag
     6181 ctatgacttt accatatcca accttatcag ttcaaccgcc ggtgcgcatg aactgtcact
     6241 gatatacgaa ttcacctcgt tttcaatcaa ttcacccaag cgaaagatga gggcgattcc
     6301 atgtcccgag ttttaaggca gtgtaattaa cttctttaaa ttagttccgt ttatttattt
     6361 gtttttatat ttgcggacta atgtttaacc aatattcaat ctgtgatgaa gaatctttcc
     6421 attgccctgt tttccgtcct gtttcttgca attaccgttc tctacatact tcatttcacc
     6481 ggaaggaaaa aggaaaccgt acgtgagtcc gtgagcagtc ccgtggaagt ccgttcggag
     6541 ggaatcgcat ttgtcaacat tgattcggta ataatgaatt ttgatatgtt ttttgaccgc
     6601 aggaatgaac tgatggagaa gcagaggaag gctgaggctg aactcacctc aaagggaagc
     6661 aggtatgaaa gggatgcaag ggattttcag gaaaaagtta ccaaaggcct ggtcaccagg
     6721 gctactgcgg cagaaatgga aaagagtctc ctccagcagc agcaggatct tatcaccctg
     6781 agggatgacc ttcagacaaa cctgatggaa gaggaacagg tcatgaacag gcagataatt
     6841 gattatataa ccacctgcct ggaacagaac aaatcggagt tcaactacca gtacgtcctg
     6901 ggcaaatcat tcggaagcgt tgtactttat ggtgatgaag cccttgatat caccagaagg
     6961 gttagtgaag ccatcaactc gaaatacagg tcggaaaaat ccaaaaaata gggaccatgg
     7021 gttttgccac caggtggctt tcggaaaaag ctctttttcc ggaattattc agggacaagc
     7081 catgtgacaa tacgggtatc atcgttgtga tacctgcata tgatgaaccg gatatagcaa
     7141 ctgtcctgga ttcgctcgcc tcatgcagcc ggccaggatg cagtgcggaa gtcattataa
     7201 tagtgaatct caggaaggat gccggcaggc aggcaatcct gaaccacagg aaaagcattg
     7261 aaaacataga agactggaag aaaaagaatg cttcctgctt tttccgcctt tttcattacg
     7321 atgccggttc tccggctgta aagaactggg gcgtcggcct tgccaggaag acaggaatgg
     7381 atgaggcact gagaaggttt gatgcggccg gtaatccgga cggtataatt gtgtgtctcg
     7441 atgctgactg tacggtaaag gagaattatt tcacggcaat tgaaaaggaa tttctgaacc
     7501 gcaggaaggc cggggcctgt tccctttatt ttgaacatcc cgtggaggat gatcccgcat
     7561 ctgtttccgc cttttatggc ataatactgt atgaactgca tctgcgttac tatgtcggtg
     7621 cactgagagc cgtacagtat ccatatgcat ttcataccgt cggttcggca atggcagtaa
     7681 gggcaagaga gtatatgagg gcggggggaa tgaaccgcag ggaggccgga gaggattttt
     7741 actttataca gaaactggtt ccgtcgggca gatatttctc ccttaattca accactgtgt
     7801 atccttcccc caggcaatcg cacagagtac ccttcgggac aggggcaaca atgggcaaac
     7861 tggcaggaga tgctgaacag cagtactata cctataatct ggatgcattc agggaactgc
     7921 agtggttgtt cagcctgaag gaagatctgt tcagctccgg caaaagggaa gcagaaagaa
     7981 tgtatgataa atttccaccg ggtttgagat attttacaga cagggatgaa tggcttgtga
     8041 aactggaaga aatcagatca aatacagccg gttatgagtc attctgtaaa agattcttcg
     8101 ggtggttcaa tatgttcaga attgtcaggt tcatgaattc ggttcatgac gggatccatg
     8161 aacgcaggcc cgttgtgcgg gcagcatgtg atcttctccg tcacacaggg catgatctga
     8221 ttccggatga tgccctgcag ctgctgaaat attacaggca gctcgacagg gagagtcagt
     8281 gattctgttt tgagtttttc ctgtcttcac ggggagtgag aacaatgtag agatcctcgt
     8341 caggcttctt catcctgtat tgttcccgtg cgaatttctc gagattgtca tttccggttt
     8401 tcagttcccg gagctttctg ctttcttcct caatcctgtt gatgtaataa tccctttgat
     8461 ttctcagctt tttcagttcg cgcatttccc tgtacctcga gatcagattg ttggaatcaa
     8521 gaaaaagtat ccatatcagg aacagaagta tggtaagaac atatttgttc ctgagaaaag
     8581 ccggtatcct gtctggtaaa ctgaaccttg ccatctcagt gcaaagctat gaattttttt
     8641 tcatctaaca cccggccggt attctgacgt gacaggttaa ttttctgccg cactgcccgg
     8701 gaaacagccg ggtcagtgag tcatccggtt gagggtgttt atgctgccgc ttgcaagaag
     8761 gcggtcatag cgatccctgg gattcctgta ataaaccagt atgtggtaat cgttctccgt
     8821 ttcggaatgg ctgccttcaa accttgatga ggttccgtcc ttcacatccc ggcttatgta
     8881 cacgtactca taattatacc agccctgctt gagcagcatg gtgcattcat actgtttcat
     8941 gccgggattg tacagcatcc tgttgtcccc gccgaaggtc cagtctgcca gcaggcccga
     9001 aacatacagc tctcctgcat caggtttttc gtatgaaggc agcgtgaaat agacgtacag
     9061 ataatctgca tcggtatccg ggtcgcggcc ttcctgccgg gcaatgatgt actttccgtt
     9121 aatgtcctgc gagtaaaaat acggcttgaa ttcgcggttg tccgacggca gaagcaaaac
     9181 gtgataatca ggggcacgga aatcaatcct ttccacattc acgccgggac gttttatgct
     9241 tttaatgtcg aaaaacctgt attcattccc tcccctgaaa atatttctgt cggagagtga
     9301 tgtatatttc agttcattgc ttccgtaaac gtcaggtttg aggtttttcc tggaagtgtt
     9361 ccatctgccg ttctgcagga tgaaggcgta aatattcctg aaaggatcaa taattccgag
     9421 tccggcaata tttatgctga aatccacctg ctgcttttcg ttcctgctgt cttccgtccg
     9481 tggaaggtga acgcttatat caattccggc agcatcctcg gttatcatga agcgcctggt
     9541 gattacaggc ctgtccggat catcaccgga ataaacatac agtatgtagt tgcctgaaag
     9601 catgggtttc agcctttcct ccgggaagat cagactgtaa tgaaaatagg agacggtggt
     9661 attgaaggaa ggtctgtatt tgcttatctc atcataggtg aagccttcca aataatcgcc
     9721 tgtgaagagc tcagacctct gccagtcctt gttgcagtga ataagggtgt agtaatatgt
     9781 ttcaggatgc tcatcaagga gatcgaaatg aaatgcaagc ctgtccggac tgttaagcct
     9841 tatcagggga tatgagagat tccattcctc cctgaagagc tgcaccgttt taatcctttt
     9901 gtcaaaaatg ctgtcggtgt aaaaatccgg gtcataggct cttcccctga taaccggctg
     9961 caggaaagcg gaaaagatga taaatatcag tattcttttc attttataga ctgcagttgg
    10021 aaatatgcaa acataaagga aaaaaattac aatcatattc agcatttttg gcaattttta
    10081 aagagctatt tatacatttg caaccaatgt tattttatta acaaatcccg gtttccgttt
    10141 tattctgcta acatggaatt aaattaaaaa atgaccagaa aaataagatt gaagaaaggc
    10201 ttggatattc ctctgcccgg gaaagctgaa aaaactgtat cggttgttgc ttcccctgca
    10261 tttttcgctg ttaagccggt tgattttccg ggacttgttc ccaagctgga tgtcaggccc
    10321 ggtgacagtg taaaggccgg tt
//
LOCUS       DAOSPX010000078        10311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6326_length_10311_cov_4.680772, whole genome shotgun sequence.
ACCESSION   DAOSPX010000078 DAOSPX010000000
VERSION     DAOSPX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6326_length_10311_cov_4.680772"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLZ75_05530"
     CDS             <1..513
                     /locus_tag="PLZ75_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOS71893.1"
                     /translation="IILDKGAGSDVHIHDMLAGTILWISWGQVLRLFLVILPIALLHY
                     FFRDKFKSVTLAYQNKESDIKNPKLWDFIFYLTFGIIIVEAVSIGGIVTIFAFLIIPA
                     AISALFSTRWTQRILIGLLTGGIAVIFGLYLSWVLDVPCSPTIILFLAVLLLLAIILK
                     SIMKIRRTAE"
     gene            557..1132
                     /locus_tag="PLZ75_05535"
     CDS             557..1132
                     /locus_tag="PLZ75_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS71894.1"
                     /translation="MTDSELITLILQGDRDSFRILVERYQQMVFRTCMGFLHNREEAE
                     DLAQDVFIRAYQSLSRFRGESAFSTWLYRIAVNASLNRTRRSSLKITFETLENTVISA
                     PATSEDDPEKILISREQSVWLRRAVNSLPENQRTAIILSKYEDLSQKEIAEIMMTSEG
                     AVESLLFRAKRNLRKRLSSESKKIEKRHRKK"
     gene            1164..1580
                     /locus_tag="PLZ75_05540"
     CDS             1164..1580
                     /locus_tag="PLZ75_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOS71895.1"
                     /translation="MNCRFYQKEIDSFLEGRSGPAAREQIESHLRECRECREYYRIQL
                     LAEELISEEKQLKVNPFLSARVMSRIKNPESLREKKVPGILKPALVVLSVAAAIFLGI
                     VMGSIPGLSRGSRPVPAELSMMNDALLESVELLAAD"
     gene            1591..2154
                     /locus_tag="PLZ75_05545"
     CDS             1591..2154
                     /locus_tag="PLZ75_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71896.1"
                     /translation="MRTGSRYHWMIWAIIALALMNVTTFATILVNRGRTVKTGMLHLP
                     EQAYSDRTSLRYSGRYFRDHLGLTREQMSRFTEFNPVFRRNAREINISLAELRRQMMI
                     EMSAETSDTVKLNTISDSIGRLHSRLKKISWEYYLDLKNICNKEQEAKLEEMFGSMFT
                     PDGQEFRRGPGGFRNPPGRGQGRRPGI"
     gene            2257..2727
                     /locus_tag="PLZ75_05550"
     CDS             2257..2727
                     /locus_tag="PLZ75_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71897.1"
                     /translation="MKRKLFISAIVLLIATALTYAREQERVQTQQNPPGRGVWVDEDN
                     NGICDNFEARAAAGFRNGIRPFMRGGRQDFETGEANRGILPGRGYCRNYTGSVNSRDA
                     GNYSNAGNRAYGRNYISAGNRGYGRNYINAGNRGYGRNYIDANNNGICDYREIP"
     gene            2684..3592
                     /locus_tag="PLZ75_05555"
     CDS             2684..3592
                     /locus_tag="PLZ75_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOS71898.1"
                     /translation="MPTTTVYVITVKFPDEYRSIAMKKQNSFNLRSFTSFSLVISTIV
                     MSWSGFILYFAPPGRIANWTGWKIMLFTKAEWQAFHTIFSFMFFILFIIHLFFVNWKA
                     FLTYFRSRIRKGLNRKRELFAAVGISLLFFTGTLASWAPFGTVMDFGETLKEGWDKKY
                     ESPAIAHMEEFTLLRLATVFAGVSSDDLLITLRDSGIVVRGQNARLKHIAADNGVTPA
                     RLYEILTRRYPGDEESHKYEVPSGIGKMTINMVASRLGKDPSVLTGILRDMGVSANGE
                     STLRTVAVQLGISPHDLYSLLTGDRN"
     gene            3820..4704
                     /locus_tag="PLZ75_05560"
     CDS             3820..4704
                     /locus_tag="PLZ75_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOS71899.1"
                     /translation="MARRKCTKIVRGQNAVDGAGVRLRRVLGIRTIKEFDPFLMLDGF
                     DSDDPRDYMNGFPWHPHRGIETVTYLLEGEIEHGDSLGNSGVIGGLQCQWMTAGSGII
                     HQEMPKESDRLLGCQLWVNLPEKDKMTRPAYRDIKQEDVPVISEKNAGIRVLSGNYRG
                     HGGAVKREYVKVQYLDVSLDPGTVWSYRETPNNQTLFLYLISGTLAADENLEEYEEKA
                     CAMLMTATSESEAENDEVRVKSGADGARFLLIAAKPLNEPVAWGGPIVMNTREELDLA
                     FRELDNNTFIKHEKPQEL"
     gene            complement(4825..5337)
                     /locus_tag="PLZ75_05565"
     CDS             complement(4825..5337)
                     /locus_tag="PLZ75_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71900.1"
                     /translation="MRHAAAVVFLLLSAVLAGQKAESDVTFADYRNLILTVKKDAMKA
                     TGKLTSGELVSVGCEQNVCFFEIQYKDRKLRQPVGDDITRLTISEFDFNSDGDMELVV
                     VNDFRATSFLYVFSYSRGIIMKLFEKEIKYDRIVIRSDYIEHHLPTGMDTVWHYYQGE
                     FWTMTPYTLP"
     gene            5642..6007
                     /locus_tag="PLZ75_05570"
     CDS             5642..6007
                     /locus_tag="PLZ75_05570"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOS71901.1"
                     /translation="MKDKKPDHTEDEYIESSKVKSVMSRMKSDRCFHDMAETFRAMSD
                     PTRVKIIFALCEEKELCVSDIASIIASSKSATSHHLRTLRNMKLVKYNKVGKIAFYSL
                     DDIHINNLFEEGLRHVEEK"
     gene            6048..6266
                     /locus_tag="PLZ75_05575"
     CDS             6048..6266
                     /locus_tag="PLZ75_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOS71902.1"
                     /translation="MIDLQEGQSGIVLSLNGGRNLTKRLADLGMGPGTRITVLRTSLF
                     SGPVQVEVTGSKLVLGRGLASKILVEPR"
     gene            6263..8197
                     /gene="feoB"
                     /locus_tag="PLZ75_05580"
     CDS             6263..8197
                     /gene="feoB"
                     /locus_tag="PLZ75_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOS71903.1"
                     /translation="MKDNKRISVVLAGQGNTGKTSIFNYYTGSHQHTGNWAGKTIEKL
                     EGTLYYKDYTIDVLDLPGIYSLTTLSVEELIAREYIIKHKPDVVINIVDATNLERNLI
                     FTLQLLELECPLIIALNMVDIAKKKGIEIDTGKLQEKFNAPVIPTIAKRGTGLTELMD
                     KVIEVKENGHEPGMIRYGREIESCVDELSAELKNTGLLYSERWIAIKLLEKDSEITNT
                     ISQIRPELIRRKETLEKRLEEIHGHDSSLVISDERSHYAAQIVSETAIMRPHRKLSLS
                     DRLDIITTHKFAGYLVMILIVGGMFLTVFKLGNWLSSVLETVFSSWHNWWIASVGETF
                     LASLSWSAIEGVLALVEIALPYILPFYIILYILEEWGYIARISFLMDNLMHKIGIHGK
                     GCISFIIGLGCNVPGCLSCRIMESRRERFLTAVLVTLVPCSAVSVIVFGLVGKYVGLI
                     WAFGLYVFAMVIIFIAGKILSKLLPGEPVELIMPMPDYKKPHLKAILYQTWDALKEFI
                     YIAAPIVIIAGVVIQAINLAGWMPAISRFMSPVTVNWLGLPAITGILLIFGILRKELI
                     LVMLAAYLGTTEFAEVLTPVQMITLAVVSMFYVPCIATVSVLWKEYGWKRALAVTLIE
                     ILFAILLAGLVYRLLSLFDL"
     gene            complement(8183..9235)
                     /locus_tag="PLZ75_05585"
     CDS             complement(8183..9235)
                     /locus_tag="PLZ75_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOS71904.1"
                     /translation="MASINTEYGRDLRKYGGFDFNACYNCGTCTAVCSLSTEEDSFPR
                     EMVRFSVLGMEDDIKSSLKPWLCYYCGECTTHCPQTANPGELMMSLRRWLTSVYDWTG
                     LSGLLYRFLPATVTAMILAAAAVIAFSASRDFNLPEIMKAGHYFEMIAIGTVLAVIFI
                     PNLIRMWWFTMLKPGIKVPAANYFKAAADLFIHMFTQKRARDCEDNNFRWLEHFLLVI
                     GYLALLFTTVVLDWFSSSSLFVIILGYLVSAIVFIVTIDFVSGRIRKKKEVSRFSHAS
                     DWMFVVWLVLMGLSAFAVRLFIDLGWLENNLWLYLLHLTVLVQWALIIVPFGKWTHFL
                     YRSFAMYFHSVKSQVK"
     gene            complement(9235..9513)
                     /locus_tag="PLZ75_05590"
     CDS             complement(9235..9513)
                     /locus_tag="PLZ75_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71905.1"
                     /translation="METEKGKTFRYLREKREVSQDAKDRLREFNRAKKEILGALKEKD
                     MTVNELGKKLDRPGHEIMFYLMTLVKYGSVEAGDIDDMDEYFSYKLKK"
     gene            complement(9526..>10311)
                     /locus_tag="PLZ75_05595"
     CDS             complement(9526..>10311)
                     /locus_tag="PLZ75_05595"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS71906.1"
                     /translation="YCSRYCCTSVIHSAIQAKKKFGDITGYHFTRGIRTYGKQELLYA
                     ESLKGGEIYLQSYEDDPPVVEVNENSLKVRINDILTGIREVSVEPDLVVLVTGMVPRA
                     DNSVGALFKLPKGRDSFFNEIHMKLRPVETVIDGIIICGACQGPKNITESVNSALSAA
                     VKSFSLVNRGELEMEPIVATIDEETCEWCGACYEACPFDAIRKTVKGTREIAEINNSV
                     CKGCGICLPVCPVDAIELTAYSNREIESMIDALAGSYPIMTTK"
BASE COUNT         2885 a         2321 c         2448 g         2657 t
ORIGIN      
        1 attattctcg acaagggcgc gggaagcgat gtacacattc atgatatgct tgcgggaaca
       61 attctctgga tttcctgggg tcaggttctc agactatttc ttgtaatact gcctatagcc
      121 ctgttgcatt attttttcag ggataaattc aaatccgtca ctcttgcata tcagaacaag
      181 gaaagcgata ttaaaaatcc aaaactatgg gatttcattt tttatctgac tttcggaatc
      241 attatagtgg aagcagttag cataggaggt atagtaacaa tcttcgcatt ccttatcata
      301 cctgcagcga tatctgccct tttcagtacc cgctggacac agagaatcct tatcggtctg
      361 ctgaccgggg gcattgccgt aatcttcgga ctctaccttt catgggtact ggatgttcca
      421 tgttccccta ccatcatact cttccttgcg gttttgctgc ttctggcaat cattttaaaa
      481 agtattatga aaatccggag gacagcagaa taattattat gatcttagga ccattgatgg
      541 tcttcattct gcccggatga ctgattctga attaataaca ttaatcctcc agggcgacag
      601 ggattcattc cgtattcttg ttgaacgcta ccagcagatg gtgttcagaa cctgcatggg
      661 tttcctccat aacagggaag aagctgaaga tctggcacag gatgttttta tccgggctta
      721 tcagtccctg tcccgtttca ggggagagtc ggctttttcc acctggcttt acaggatcgc
      781 cgtaaacgct tcacttaaca ggaccaggcg gtcttccctg aaaattacat ttgaaacact
      841 ggaaaacacg gtcatttccg ccccggcaac aagtgaggat gatcctgaaa agattctgat
      901 cagccgggag cagtcagtgt ggctcaggcg ggctgtcaat tcacttcctg aaaatcagag
      961 aactgcaatc atactgagca aatatgagga tctgtcccag aaagagattg cagaaataat
     1021 gatgacaagc gagggagccg tggaatccct gttgttccgc gcaaaaagaa accttcgtaa
     1081 aagattgtca tccgaaagta aaaagattga aaagaggcac aggaaaaaat aaaattttgt
     1141 ttctaaccat aaaaagcgtt gagatgaatt gtcgttttta tcagaaagaa attgattcct
     1201 ttctggaagg aagatccggt cccgccgcaa gggaacagat tgaatcacat ctcagggaat
     1261 gcagggaatg cagggaatat tacaggattc agctgctggc cgaagagctg atatcagaag
     1321 aaaaacaact gaaggtaaac cctttccttt ctgcacgcgt gatgtcacgg attaaaaatc
     1381 cggaatccct cagagagaaa aaggttccgg gcatactgaa accggccctg gtggtattgt
     1441 cggttgccgc agcaattttt cttggtatcg tgatgggaag tattcccggc ctgagccggg
     1501 gcagcaggcc tgttcctgct gaactgagca tgatgaatga tgccctgctg gaatcggttg
     1561 aactgcttgc agctgattaa aatgaacatc atgagaaccg gcagcagata ccactggatg
     1621 atatgggcaa taattgcact ggccctgatg aatgtcacca cctttgcaac catcctggtc
     1681 aacaggggca gaacagtaaa gaccggcatg cttcacctgc cggagcaagc gtactcagac
     1741 aggacttcac tccgttacag cggaagatat ttccgcgacc acctgggtct gaccagggaa
     1801 caaatgtcga ggttcacgga attcaatccc gtattcagaa gaaatgcaag agaaataaat
     1861 atcagcctgg cggagctgag aaggcagatg atgatcgaaa tgtctgcgga aacgagtgat
     1921 accgtaaaac tgaataccat ttcggattca ataggccgcc tccacagccg cctgaaaaag
     1981 ataagctggg aatattatct tgacctgaaa aatatatgca ataaggaaca ggaggcaaaa
     2041 cttgaagaaa tgtttggctc aatgtttacg cctgacggtc aggaattccg gcgcggtccc
     2101 ggtggattcc ggaatccgcc cggccggggt cagggcagac ggcccggcat ctgaaaggga
     2161 ataatttttt taaatacgca ggaaaatgca ggattatgtt tccaataaca aaggcattcc
     2221 tttaaaacct tatgtcaaat ttaaaatcac atatcaatga aaaggaaact ctttataagc
     2281 gcaatcgtac tgctgattgc aacagcactg acttatgccc gggaacagga aagggttcag
     2341 acgcagcaga accctcctgg caggggcgtg tgggttgatg aagacaataa cggcatttgc
     2401 gacaattttg aagcacgtgc agcagcggga ttcagaaacg gcatccggcc tttcatgaga
     2461 ggaggccgtc aggattttga aaccggggaa gctaaccgtg gcatcctgcc cggacgcgga
     2521 tactgcagaa actataccgg aagcgtaaac agcagagatg caggaaacta ttccaatgca
     2581 ggaaacaggg cttacggaag aaactacatc agtgccggga acagaggcta cggaagaaac
     2641 tacatcaatg ccgggaacag aggctacgga agaaactata ttgatgccaa caacaacggt
     2701 atatgtgatt accgtgaaat tccctgatga ataccggtcc attgcaatga agaaacagaa
     2761 ttcctttaat ctccgcagct tcaccagttt cagcctggta atctcaacga ttgttatgtc
     2821 atggtccggt ttcattctct atttcgcgcc tcccggaaga atagccaact ggaccggctg
     2881 gaaaattatg ctcttcacaa aggctgaatg gcaggccttt catacaatat tctcattcat
     2941 gttcttcatc ctgttcatca ttcatctgtt ttttgtgaac tggaaggctt ttcttaccta
     3001 tttcagaagc aggatcagaa agggcctgaa cagaaaacgg gagctttttg cagcagtggg
     3061 catttcactt ttatttttca ccggaaccct cgcatcctgg gcacctttcg gtactgtcat
     3121 ggatttcggc gaaacactga aggaaggctg ggataaaaaa tatgaaagtc cggcaatagc
     3181 acatatggaa gaattcacac ttttgaggct tgcgacagtt tttgcgggag tcagctccga
     3241 tgatcttctc atcacactga gggacagtgg tattgttgtc agggggcaga atgccaggct
     3301 gaagcatatt gctgctgaca acggggtgac ccctgccagg ctgtatgaga tactgaccag
     3361 gagatatccc ggagatgagg agagtcataa atatgaagtg ccttcaggaa tcggtaaaat
     3421 gaccataaac atggtagctt caaggctcgg gaaagatcct tcagtactga caggaatttt
     3481 aagggatatg ggtgtcagtg ccaatggtga aagcaccttg agaacagtag ccgttcagct
     3541 tggaatatct ccccatgatc tttactccct gctgacagga gaccggaact gacatattcc
     3601 tgtgaactga ttccggcaat ctgccggagt atgccataag aaaaccccgt acagacccgg
     3661 agccagaaga tttttcctgc atttctgaac attaccggcc taaatcggtt tattacaata
     3721 cagctacatc cggatatatt gaaatccaat ttagaaaaac cattaccggt aaatacaaaa
     3781 ccgggcagat gatgataaat caaaattaaa ggataatcca tggccagaag aaaatgtaca
     3841 aaaattgtca gggggcagaa tgccgttgac ggtgcgggtg tccgcctgag gagggttctg
     3901 gggatcagaa caataaaaga attcgatccc tttcttatgc ttgacggctt tgattcggat
     3961 gatccccgcg attacatgaa tggctttcca tggcatcccc acaggggaat tgaaacggtc
     4021 acctacctgc tggaaggtga aattgaacat ggggacagtc tgggaaacag cggcgtcata
     4081 ggcgggctgc agtgccagtg gatgaccgcc ggttctggta tcattcacca ggaaatgccg
     4141 aaggaatccg acaggttgct gggatgccag ctctgggtaa acctcccgga aaaggataaa
     4201 atgacccggc ctgcataccg ggatataaaa caggaagatg tgccggtgat aagcgaaaaa
     4261 aatgcaggca tcagagtcct gtccggtaat tacaggggcc acgggggcgc ggtaaaaaga
     4321 gaatatgtaa aagtgcagta tcttgatgtg tcactggatc ccggcacagt atggagctac
     4381 cgggaaactc ccaataatca gacattgttt ctttacctga tcagcggaac ccttgctgct
     4441 gatgaaaacc ttgaggagta tgaggagaag gcctgtgcaa tgctgatgac tgccacttca
     4501 gaaagtgaag cggaaaatga tgaagtgagg gtaaaatcgg gcgcagatgg tgcaaggttc
     4561 ctgctgatcg ccgcaaaacc gctgaatgag ccggttgcat ggggaggtcc gatagtaatg
     4621 aacacacggg aagaacttga cctggctttc agggaacttg acaacaatac ctttatcaag
     4681 catgaaaagc cgcaggagtt atgatttatc atgaacctgg cacaagcgct ccggcatgcg
     4741 gacgatatgg ttcagaagcc ggttctctcc ttctgatggt catgtattaa acaggagtga
     4801 gacgccggat gtcaggcagt catcctacgg cagggtatag ggggtcatgg tccagaattc
     4861 tccctggtaa taatgccata cggtatccat gcctgtagga agatgatgct caatatagtc
     4921 gctcctgata actatcctgt catatttaat ctctttttca aagagcttca tgataattcc
     4981 cctgctgtat gaaaagacat aaaggaagga tgtagccctg aaatcattta ctacaaccag
     5041 ctccatatca ccgtcggaat taaagtcaaa ctcggatatt gtaagtctgg taatatcatc
     5101 tcccacaggt tgtctcagct tcctgtcctt atattgtatc tcaaaaaaac aaacattctg
     5161 ctcacatccc acgcttacca gctctcccga agtaagcttg cccgtggctt tcatagcatc
     5221 cttcttcaca gtaaggatga gattcctgta atcggcaaat gtaacatctg attctgcctt
     5281 ctgcccggca agaacggcgg aaagcaacag gaaaacaaca gctgctgcat gtctcatggc
     5341 aaacaagatt agtaaatacg ctaaaagttc aagttatcct ggcaattctc tcctcagaaa
     5401 ttccagaaag ctctcaagat aagaaattta aattaaatat gttccaacag gtcagcagaa
     5461 taaatattac aatgttcccg gcggccccgc atgaatgccc gaaattctgt aaaagccgga
     5521 aaacagcggt tctgcaaatg tgattccggc tttgctataa atcatatgga attgtctttt
     5581 ttgtttcgtg gaaaagatta tttttgcgaa cgtttgaaca gttgctcata tataaaaata
     5641 aatgaaggat aaaaagccgg atcatactga agatgaatat atcgagagca gcaaggtgaa
     5701 gtctgtgatg agcaggatga aatcagacag gtgttttcat gatatggctg aaacattcag
     5761 ggcaatgagt gatccgacga gggtaaagat catatttgct ctttgcgagg aaaaggaatt
     5821 atgcgtcagt gatatagctt ccataatcgc ttcatcaaaa tccgcaacat cgcatcatct
     5881 caggacactg cggaacatga agctcgtcaa gtacaacaag gtggggaaga tagcattcta
     5941 ttcgcttgac gacatacata taaacaacct ctttgaagag ggattgagac acgttgaaga
     6001 aaaataacat ctgttttaat atggatacct caggaaaacc caggagtttg attgatttgc
     6061 aggaaggtca gtctggcatt gtattgtcat taaacggagg ccgtaacctt accaaacgtc
     6121 tggcagacct ggggatgggg ccgggaacaa ggattacggt tttaaggact tccctgtttt
     6181 caggtccggt acaggtggag gttaccgggt cgaagctggt actgggcagg ggcctggcat
     6241 caaaaattct tgtggaacca cgatgaaaga caataaaagg atcagtgttg tgcttgccgg
     6301 tcagggcaac acgggcaaaa catccatatt caactattat acgggatcgc atcagcatac
     6361 cgggaactgg gccggcaaaa ccatagagaa gcttgagggg acgctctatt acaaggatta
     6421 cacaattgac gtccttgatc ttcccggcat ttattccctt accaccttat ccgtggagga
     6481 gctgattgcc cgggaataca tcataaagca caagcctgat gtggtgatca acattgttga
     6541 tgccaccaat ctggaaagga atctcatctt tacccttcag ctgcttgaac tggaatgtcc
     6601 tcttatcata gccctcaaca tggtggacat tgccaaaaag aagggtattg aaatcgacac
     6661 aggaaagcta caggaaaagt ttaatgcacc cgttattccg accatcgcaa agcgcggaac
     6721 gggcctgacc gagctgatgg acaaggtaat tgaggtaaag gaaaacgggc atgagcccgg
     6781 aatgatccgc tatggcaggg agattgagag ctgtgttgat gaattgtcgg ctgagcttaa
     6841 aaataccgga cttctttact ctgaaagatg gattgccata aaacttcttg aaaaggacag
     6901 cgagatcaca aataccatca gccagataag gcctgaactg attcgaagaa aagagactct
     6961 tgaaaagagg cttgaagaaa ttcacgggca tgactcctcg ctggttattt cggatgaaag
     7021 gagtcattat gccgcgcaga tcgtaagtga aacagcaata atgcgtcccc acaggaaatt
     7081 atccctcagc gaccggctgg atattatcac tacccataaa tttgcaggct atctggtgat
     7141 gatactcatc gtcggcggta tgttcctgac agttttcaaa ctcggcaact ggctgtcatc
     7201 ggttctggaa acagtttttt ccagctggca caactggtgg attgccagtg taggggaaac
     7261 attccttgct tctttgagct ggtctgccat tgaaggtgtc cttgccctgg ttgaaattgc
     7321 ccttccctat attctgccct tttatataat cctatatatc ctggaagagt ggggatacat
     7381 cgcccgtatt tcatttctga tggataatct tatgcataag attggtattc acgggaaagg
     7441 ctgtatttca ttcatcatcg gccttggctg caatgttccc ggctgtcttt cctgccggat
     7501 aatggaatcc aggcgtgaac gcttccttac tgctgttctt gtaactcttg tgccctgctc
     7561 agccgtttcg gtgattgtat tcggcctggt cgggaaatat gtgggcctca tctgggcatt
     7621 cggcctgtat gtgtttgcaa tggtgataat tttcattgcg ggaaagatat tgtcaaagct
     7681 tcttcccggg gaacctgttg agctcatcat gccgatgccc gattataaga agccgcatct
     7741 gaaggccatc ctttaccaga catgggatgc attaaaggaa ttcatctaca tagctgctcc
     7801 catagttatc atcgccggtg ttgtgattca ggccatcaac ctggcaggtt ggatgcctgc
     7861 aatatcccgc ttcatgagtc ccgttaccgt taactggctg ggacttccgg caataacagg
     7921 gatccttctg atattcggta ttttaagaaa ggagctcata ctcgtcatgc tggcagcata
     7981 tctgggaaca acagaatttg cagaagtgct cactccggtc cagatgatta cccttgccgt
     8041 ggtaagcatg ttctacgttc cctgtatagc cacagtatcg gttctgtgga aggaatacgg
     8101 atggaaaagg gctcttgccg tcacccttat tgaaattctg ttcgcaatcc tgctggcagg
     8161 cctggtatac aggcttctca gtctatttga cctgtgattt cacactgtga aagtacattg
     8221 caaatgaccg gtaaaggaag tgtgtccact tgccgaaagg tacaatgatc agggcccact
     8281 gcacgagcac ggtcagatgc agaagataga gccacagatt gttctccagc catcccagat
     8341 caatgaagag gcgtactgcg aaggcagaca atcccataag tacaagccat acaacaaaca
     8401 tccagtcgga tgcatgagag aatctgctca cctccttttt ctttctgatc cggccggaaa
     8461 caaaatcaat tgtaacaatg aatacaattg cgcttacaag ataaccaagg ataatcacaa
     8521 agaggctgct gctcgagaac cagtcgagta caaccgtggt aaacagcagt gcaagatagc
     8581 ctatcaccag caggaagtgc tcgagccatc tgaaattatt gtcttcacag tcccttgccc
     8641 ttttctgtgt gaacatgtgt atgaacagat cggctgccgc tttgaaataa tttgctgccg
     8701 gtacctttat tcccggtttc agcattgtga accaccacat ccttatcagg ttgggaataa
     8761 aaatgaccgc aagaaccgtt ccgatggcaa tcatttcgaa atagtggcct gctttcatta
     8821 tctccggaag gttgaaatcc ctggaagcgc tgaaggcgat aacggctgct gctgccagta
     8881 tcatggctgt caccgttgcc ggaaggaatc tgtaaagaag gccggacagg ccggtccagt
     8941 cgtacactga ggtaagccat ctcctcagcg acatcatcag ttcgcccgga ttggccgtct
     9001 gcggacagtg tgtggtacat tcgccgcaat agtaacagag ccatggttta agtgatgatt
     9061 taatatcatc ttccattccg aggacactga atctgaccat ttccctggga aaggaatcct
     9121 cttcagttga aagtgaacag accgcggtac aggtgccgca gttataacag gcattaaaat
     9181 caaatccgcc gtatttccta aggtctcttc cgtattcagt atttattgaa gccattattt
     9241 cttgagtttg taggaaaaat attcgtccat gtcatctatg tcgccggctt caacactgcc
     9301 gtactttacc agtgtcatca ggtaaaacat aatttcatgg ccgggtctgt caagtttttt
     9361 tccaagttca tttacggtca tatccttttc cttcagtgcg cccagaatct cttttttggc
     9421 cctgttgaat tccctgagcc tgtctttggc atcctgtgaa acttcgcgtt tttccctcag
     9481 atacctgaag gtttttccct tttctgtttc catgagcgag aatgattatt ttgttgtcat
     9541 tatcggataa cttcctgcca gggcatctat catgctttct atctccctgt ttgaatatgc
     9601 tgtcagctct attgcatcaa cggggcagac gggaaggcaa atgccgcatc ccttgcaaac
     9661 tgaattattt atttcggcta tttccctggt tcccttcact gttttcctta ttgcatcaaa
     9721 ggggcaggct tcgtaacatg ctccgcacca ttcacaggtt tcctcatcta tggttgcaac
     9781 tatcggctcc atttcaagct ctcccctgtt aaccagggaa aaggatttga cagccgcgga
     9841 aagcgcggaa ttgactgact ccgtaatatt tttcggaccc tggcatgcac cgcagattat
     9901 aattccgtca atcacagttt ctaccggccg cagtttcatg tgtatctcgt tgaagaagct
     9961 gtcccttccc tttggcagtt tgaagagggc acccaccgaa ttgtcagccc tcggtaccat
    10021 tccggtaacc aggaccacaa gatccggctc aacggaaact tcccgtatcc cggtgagaat
    10081 atcatttatc ctcactttta aactgttttc atttacttca actacaggcg gatcgtcctc
    10141 ataggactga aggtaaattt ctcctccctt cagggattcc gcgtacagaa gttcctgctt
    10201 gccgtaggtc ctgatacccc gcgtgaaatg gtagccggta atatccccga atttcttctt
    10261 cgcctgtatt gctgaatgaa taaccgatgt acagcaataa cgtgaacaat a
//
LOCUS       DAOSPX010000079        10281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6345_length_10281_cov_4.202719, whole genome shotgun sequence.
ACCESSION   DAOSPX010000079 DAOSPX010000000
VERSION     DAOSPX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10281
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6345_length_10281_cov_4.202719"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLZ75_05600"
     CDS             <1..328
                     /locus_tag="PLZ75_05600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS71907.1"
                     /translation="YLNTFDILIHTSFAEGSPNLIKEAMACNMPIVATDVGDISWLFG
                     DEPGHFLTSFEPVDVAEEITQAIEFSKKYGRTKGRDRIIKLGLDSETIADRIIEVYNS
                     VLENGK"
     gene            318..1484
                     /locus_tag="PLZ75_05605"
     CDS             318..1484
                     /locus_tag="PLZ75_05605"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71908.1"
                     /translation="MGSKPGAVIIEGHVQGLSNTRSLGEAGIPVFVVDKNDCLARFSK
                     YCLKFFRCPDYLTDEFTDFLIEIAKNNNLNGWVLIPSNDHAVFTISRHKNKLEKYYRI
                     ITPALNIIENIYDKTRLFRIAENEGIPYPLTYNIKKIEDADQFEISFPVIIKGRFGLS
                     YYKKFGKKAFFATDETSLKKYLKVIKKNFELENAIIQEFIPSDKTNKTISFTSFCEDG
                     EIKTFWMGAKLREHPLRFGTATFAESVYVEECYLNSINLLRALNYTGVSEIEYLKDPR
                     DGKYKLIEINARTWLWVGLAKECGIDYAKYIYDYLNDIPVGFPAEYKLNTKWINYFTD
                     TIFSIKAIVTGKLRISEYLKSLKGEKTAAVFQKEDIMPGIMFLALMPYIMVKRT"
     gene            1494..2312
                     /locus_tag="PLZ75_05610"
     CDS             1494..2312
                     /locus_tag="PLZ75_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOS71909.1"
                     /translation="MTVMEFIIIVLSTFLLSTALFVFWIVKGGFNRKFLYTDNVWLAS
                     AASVLNIISYSFIDMPLWLATPFISGFIIILLFTVLFFYRFFRNPHRIIPGDQNDIVS
                     GADGRIIYIKELEKDQIPVTVKKLRIADLSEITKTNILKRPCYLIGIAMTLFDVHVNR
                     SPIDGKIVLVKHTDGTAIGLNTPVSTITNERNTVVIEREDGVIAGVVQIAARGVRRCI
                     IMKKPGDIVERGSIIGKIRWGSQFDMIIPRNCEIKIREGEQVYAGSTIIARLLD"
     gene            2318..3400
                     /locus_tag="PLZ75_05615"
     CDS             2318..3400
                     /locus_tag="PLZ75_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HOS71910.1"
                     /translation="MRILVDIGHPAHVHLFRNFAIIMMNRQHQILFTCREKEHVVKLL
                     EHYDLPFVILGKIPESIQGKVSSILKFGYGIYKVAYRFKPDLFLSHGSHYAAHVAFVT
                     GKPHIAFEDTFNFEQIWLYKPFTKAILTGDYEHPLKSSKVVRYSGCHELAYLHPNRYK
                     PDISVLDEMGISVNEKYVILRFSSWKASHDYGHKGMNFNNKLEAVKQFSQYAKVFISS
                     ESELPPILQTYKINIAPFRIHDALAYASLILADSFTMPSESTVLGTPAVIIHNNRSLC
                     LEEQSEKYRLCYIFSESENDQQEAIKKGLELLKKENIKTDWNKRRNKMLKDKIDVTSM
                     LIWFIENWPESFYIMQENPDYQERFR"
     gene            complement(3988..4719)
                     /locus_tag="PLZ75_05620"
     CDS             complement(3988..4719)
                     /locus_tag="PLZ75_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71911.1"
                     /translation="MDARKMNSLLFAQLENKFGIKGTYYFRITPESFDENIIKQIYDL
                     GHEIGYHYEDVSLIAKTRSIFNVQRYNTNRDKALLIKASIDSFSKNLAKLRRIVPVET
                     VCMHGSPLSKWDNRLLWKYYNYRDFGIKGEPYFDTDFGEVFYLTDTGRRWDGEAFSVR
                     DKTTRKETNKTSGTEAIKGGNSLHSTFDIINALKENRLPEKIMLTVHPQRWDSRPLVW
                     FRELIWQNVKNIVKGTLVKIQNDKL"
     gene            6349..>10281
                     /locus_tag="PLZ75_05625"
     CDS             6349..>10281
                     /locus_tag="PLZ75_05625"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71912.1"
                     /translation="MPNIEPRHVQYVYGTHTAKGIPNVYIDVHGTMVKVTDNNGVAIS
                     GVWHVNPDGTTNATGYTTPSGFFEGPVIQYLWDAGTKTLVTPLAQTYPIYHSGDYIND
                     KENDIFDVTLRNWGPCNPYDPMDPFTYNNSVNDFSRLRLVGAPPLPTASDVTVCSGTP
                     VSLSAIRNGTNPGQLHWYNNADLHPGHQVGTGTTYSPGILADGTYNYWVREIGTTGQF
                     CEGPAKQVTVTVRRLLQTPPNISGPTNTCPNVKDLVFSVPHNPPVMSPGGPTRYVWSV
                     PAGLTIKSGQGTRSITVDVGTTVGNKTISVYLEYTSDPSCNSNPSTIQLTVFSVNHNP
                     GTIRGSNICSGEVVNITNNASASTGNPSSGGLTYSWERAAGPTFSVWTPLTGSSATFS
                     ETISTPGVYRYRRTATFNCGDPVSTTRDITVFSITHNGGAISGTDICQGGTVNITNQT
                     SAFTGTPASSGPTYSWHRAPGPAFSSWTLLSGSSATFSEIVNTPGTYRYRRTATFGCG
                     NPRTAIVDITVNPLPAPVISGETSPCHGNTKVYTTQYHDGHNYSWSVVNGTISGSSTG
                     NSVTVIWNLSSGTGSLRVTETIQSSGCAVTTPNYNITINPAAPGAAGAITGLSNMCYA
                     QTGVTYSILPVANASNYVWSVPAGVNIVSGQGTTSLKVDFTSAAVSPVTISVYPENGC
                     GAGGTSSRVITIYPELEGGSIGGDQTICYGYDVAAFTSSGAATGGAGSFSYLWQYTTD
                     MSAPLGSSAWQNISSATGLTYDHGTLTGTTRFVRRAVEGTCSTPVYSNIVTVTVRPQL
                     NGGSIGSDQTICYGADVAAFTSSGAATGGAGSFSYLWQYTTDMSAPLGSSAWQDISSA
                     TGLIYDHGTLTGTTRFVRRAVEGTCSTPVYSNIVRVTVRPELKGGLISGNQTICYGTI
                     PAQFTNSTAASGGAGSFTYSWWYTTDAGLDAGDPGWNEISSSNTTGFNYNNTLTTTTR
                     FVRRAVDGTCTTPVYSNIVTVTVRPQLDGGEVGTSQTVCYGNDVPGFTSKSPATGGEG
                     SFTYTWQYTTNMLATPGDGNWTDIGSSNSVTYDHGNLTTTTKFVRKAVESTCSPVVYS
                     NEIIITVMPALNSGVIGSSHFICYGEAPNPFSSLVPASGGGGSFTYTWQYTTNTSAVA
                     GDGNWTDIANSDDEAYTHGVLTATTLFVRKAEDASCTTPVYSNIITVTINPLPVTSLI
                     RGPSVLCEDATNRVYEVDNHPGSTYTWTVPSSMVITSPPGMYFIIVDAVPGMAAPGDT
                     ITVTETILSTGCVGKPVKLPVSVVPIIPGVVVNGPASLCLGDTAVYSVP"
BASE COUNT         3083 a         1971 c         2107 g         3120 t
ORIGIN      
        1 atatttaaat acatttgata tattgataca tacatctttt gcagaaggat cgcccaatct
       61 aatcaaagaa gccatggcat gcaatatgcc tattgtagct acagatgttg gagatatcag
      121 ttggcttttt ggcgatgaac ccggacattt tctaactagt tttgaaccag ttgatgtggc
      181 agaagaaatc acacaggcga ttgaattttc taaaaagtat ggcaggacta aaggtcgtga
      241 tcgtattatt aaactgggtc tggattcaga aactatagcg gataggatca tagaagtata
      301 taattcagtt ttggaaaatg ggaagtaaac ctggcgctgt tataattgaa ggtcatgtgc
      361 agggtttgtc caatacccga tcattgggag aggctggaat cccggtattt gtggttgata
      421 aaaacgattg tttggcacgg ttttccaaat actgtttaaa gttttttcgt tgtccggatt
      481 atcttactga cgagttcaca gattttctaa tcgagattgc aaagaacaat aatctcaatg
      541 gctgggtgtt gataccttca aatgatcatg ctgtgtttac aatatcaaga cacaaaaata
      601 aacttgaaaa gtattacaga ataattactc ctgcattaaa tatcattgag aacatatatg
      661 ataaaacccg cttattcagg atagcggaaa atgagggaat accctatccg ctgacttata
      721 atattaaaaa aattgaggat gcagatcagt tcgaaatcag ttttcctgta attatcaaag
      781 gccgttttgg tctttcatat tataaaaaat ttggtaaaaa agcatttttt gcaaccgatg
      841 aaaccagttt aaaaaaatat cttaaagtaa tcaagaaaaa ctttgaactt gaaaatgcga
      901 tcattcagga atttatacct tccgataaaa caaataaaac tatatcattc acttcattct
      961 gtgaagatgg cgaaattaaa accttttgga tgggagcaaa gctgcgtgag cacccactac
     1021 ggttcgggac agccacattc gcagaaagtg tttatgtgga agagtgctat ttgaattcca
     1081 ttaacctgct gagagctctt aattataccg gagtatctga aattgaatat ctgaaagatc
     1141 ctcgagacgg aaaatataag ctcattgaaa taaatgcgcg gacatggctg tgggtaggcc
     1201 ttgcgaaaga atgcggaatt gattatgcaa aatatatcta tgattatctg aatgatattc
     1261 cggtaggctt cccggctgaa tataaactta atactaaatg gattaattat tttacggata
     1321 ccattttcag cataaaagca attgtgaccg ggaaacttcg gataagtgaa tatctgaagt
     1381 cgttaaaagg agagaagaca gctgctgttt ttcaaaagga agatataatg cccggaatta
     1441 tgtttttagc tttaatgcca tacattatgg ttaaaaggac gtgacttatt agaatgacag
     1501 ttatggaatt tattattatt gttttatcaa cttttctgct ttcgacagca ttatttgttt
     1561 tctggattgt aaaaggtggt ttcaaccgta agttccttta cacggataat gtgtggctgg
     1621 cctcagccgc atctgtacta aacattatta gttattcatt tattgatatg cctttatggt
     1681 tggcaacccc atttattagt ggttttataa ttatattatt atttacagta cttttttttt
     1741 acaggttttt caggaatccg catcggataa ttccgggaga ccaaaatgat atagtatcag
     1801 gtgcagatgg gcgtatcatc tacataaagg aacttgaaaa agatcagata cctgtaacgg
     1861 ttaaaaaatt aagaattgct gatttatctg agatcacgaa aacaaacatt cttaaaaggc
     1921 cctgttatct gataggtatt gccatgacat tatttgatgt gcatgtaaac aggtcaccaa
     1981 tagatggaaa gattgttctg gtaaaacata cggacggcac ggcaataggg ctaaatacac
     2041 ctgtgtccac tataaccaat gaaagaaata cagtcgttat agaaagagag gatggtgtca
     2101 ttgccggtgt ggtacagatt gctgccagag gagtcagaag atgtattatt atgaaaaagc
     2161 ctggagatat tgtcgaaaga ggcagtatta tcggaaagat cagatgggga tcacagtttg
     2221 acatgattat accacgtaat tgtgagatta agattcgtga gggagagcag gtatatgccg
     2281 gcagtaccat tattgccaga cttcttgact agccaatatg cgtattttag ttgatattgg
     2341 tcatcctgcc cacgttcatc tctttcggaa ttttgccata attatgatga acaggcagca
     2401 tcaaattctt tttacctgcc gggagaaaga acatgttgta aaattacttg aacactatga
     2461 tcttcctttt gttattttag gaaaaattcc agaatctatt caaggcaagg tttcaagtat
     2521 attaaaattt ggttacggga tctacaaagt agcataccgg tttaaacccg acttgttcct
     2581 aagccatggg tcacattatg ctgctcatgt tgcgtttgtc acaggtaaac ctcatattgc
     2641 ctttgaggat acttttaatt ttgaacagat atggctttac aaacctttca caaaagctat
     2701 tctgacaggg gattatgaac atccgttaaa atcctcaaaa gttgtcagat attctggttg
     2761 tcatgaactt gcatatcttc atccaaacag atataaacct gacatttcag ttcttgacga
     2821 aatgggtata tcagtaaatg agaaatatgt aatactgcga ttcagttcgt ggaaagcaag
     2881 tcatgattat gggcataaag gcatgaattt taataataag cttgaagccg taaaacaatt
     2941 ttcacaatat gcgaaagtgt ttatctcatc agaatcagaa cttcccccga tattgcaaac
     3001 ttacaaaatt aatattgcac cattcagaat ccatgatgca ttggcgtatg catcattgat
     3061 attggcagat agttttacca tgccttctga gagtactgtt ctcggaactc ctgccgtcat
     3121 tattcataac aataggagtc tttgtttgga agagcaaagt gaaaaatatc gtttatgtta
     3181 tattttttct gaatcggaga acgatcagca ggaagcaatt aaaaaaggac tggaactatt
     3241 aaaaaaggag aatataaaga ctgattggaa taaacgtcgg aataaaatgc tgaaggacaa
     3301 gatagatgtc acttcaatgc ttatctggtt cattgaaaac tggccggaaa gtttttatat
     3361 tatgcaggaa aatccggatt atcaggaaag gtttagatag ttgcacagag aatataaaat
     3421 gcatttgttg ctattgcctg catactttgc tgccacaaca ttcgaatcaa acggtttgca
     3481 atatataatt catccataat atttatttga aacaattatt ttatcgtata attttctggc
     3541 tgggatatct tgctgtttta ttaacgactt ttgtcccgat taggaaaatt agcttagaca
     3601 agatatttct cgggccggaa gtattcagca tacgcctcga ccatcttctt cattttgcgg
     3661 tttacttcct tatttgtctt tattatttag taggtcaact taagaggatt tccctttttt
     3721 ctgtaaatcc tttcccgaaa tttgtcagac ttattttaat actcgcagta gccaccgaat
     3781 taattcaatt atgggttcct ggcaggacct tcaatgtgtt tgatatgtta tcgaatgtaa
     3841 ttggattggt tgctggggtg ggggtaataa gaatggtgct cggcaacaga tacagagtat
     3901 tgaataagat agatcaacaa gaataagaaa acctgaagga ttaaaatttc agaaacctgg
     3961 aaagactttt catttcatat ttgtaaatca taacttatcg ttttgaattt ttacaagagt
     4021 tccttttaca atgtttttaa cattctgcca gataagctcc cggaaccaga ctaagggtct
     4081 actgtcccat cgttgcggat gaacggtcaa cataatcttt tcgggcagtc tgttttcctt
     4141 tagtgcattt attatatcga aagttgaatg aaggctgttt cctcctttaa ttgcctctgt
     4201 tcctgaggtt ttgtttgtct cttttcttgt tgttttatcc ctgaccgaaa atgcctctcc
     4261 gtcccatctt ctcccggtat ctgtaagata aaacacctcc ccgaaatcag tgtcaaaata
     4321 gggttctcct tttataccaa aatccctgta attataatac ttccacaaca gccggttgtc
     4381 ccatttactc agaggactcc catgcataca taccgtttca accggcacaa tccttcttaa
     4441 ttttgcgaga tttttactaa agctgtcaat tgaagcttta attagcaaag ctttatccct
     4501 gtttgtgtta taacgttgca cattgaagat acttcgcgtt ttggcaatta agctaacatc
     4561 ttcataatga taccctatct catggcctaa atcatatatc tgtttgatta tgttttcatc
     4621 aaagctttca ggggtaattc tgaaataata agtaccctta atcccaaatt tgttttctaa
     4681 ttgtgcaaat aaaagcgaat tcatttttct tgcatccacg tcatgacgta agataacagt
     4741 cctggatttt gcgtttgtaa agaaatctat aaatgactga aagttataat tctgtttcag
     4801 aaaagatttg agaagtaact tatatgtttc aggggtgaaa tcacgcatat tgtaatgtct
     4861 tcggcataaa tttagaaaaa aaagaaagaa gattcagcta tatgcaccgt cattaaccgt
     4921 tccttaagac catcaagttc catgtacact tcctgccact aatataccat tcaataattc
     4981 attacaaaaa taaacacaag aacagaacat ataggtattt tttgcgtaca aatgatcgtg
     5041 aaagtcaaac tatagttgtt ttcatttgta aattatgttc tgctatccct tacggattgt
     5101 tgttccgtta tatctagaat cgacctttaa ttataatagg attaatgacc ggttaatcat
     5161 ttatgattat cttctcaacc ctgattgttt aggcaacgcg gtaacaggat ctcctttata
     5221 tcgaatttat aaattcttcg ttagccaaaa gtttagcgat cttatctact tattcagtaa
     5281 agttcaccta tgcaaccttt ttaacaagag gttagtctta atcttcagca atactgtcaa
     5341 taatatatct gcaaaaaata atctgtcatg aagctgtcaa ttgcgtctgg gattaatatc
     5401 aataaacaga taatcagaaa agtacacttt cttctaatta tctttattgt atcatcttta
     5461 aatatatttt cgcagtctta tcttattcct gttaatgctc caagtaacag atatcctatc
     5521 acttttactg atgttgatca gcaagtgctt attgtagaat ttgacagagc agttgttaat
     5581 gcaggaacat ccaatggctg gtcaataaca ataggaggct cgccggttgc catggttggc
     5641 aaccctgtgt cggcaggaaa taatttaaga attgcacttc cttatccaat atcttacgcg
     5701 aacagaaata cagttcttgt ttcccatact gcttctctgg gaacgctgac attgacagga
     5761 ggtgttaagc ctaatttctc aaatgttcag gcagtgaata actatattgc tgttgctgct
     5821 gattttacca acggcctgta tggtgagata gccccaaagg atatttgtgc agcggtaact
     5881 gatgttgagg ttgaatttaa ttttacatta agtaaaagat tccgcaacag tatccattat
     5941 tgtgttcctc aagtattcat acagtggcag tatccagctg cattacctag aacaattgca
     6001 ccctttaatg aaattggcgg agtaggatca ggaatttatc gtgcaatcca tacgtacgct
     6061 tcttatcctg ataatacaga taattgcaca tggaacgtat caatatatcc acatttatgg
     6121 agttcaggca cacttcagcc taatcttatg gtaactcaaa atatggttat catcattctt
     6181 ccaaactata aaagagataa tgggacccct gtaccgggga ctggagattt agaattatat
     6241 ccgcctgttg atgatccccg tactttattt tgcgtgggtg aagacattac agcttttagg
     6301 tttagagatg caactgtatt tgattgccag ttgccggttg caccggattt gccaaatatt
     6361 gaacctagac atgtacagta tgtttatggc acacatactg cgaaggggat accaaatgta
     6421 tatatagacg tacatgggac catggttaaa gtgacagata acaatggtgt ggctatatca
     6481 ggtgtttggc acgtaaaccc ggatggaaca acaaatgcaa caggttacac aactccatca
     6541 gggttttttg aaggacctgt tattcagtat ctttgggatg cagggactaa aactttggtc
     6601 actcctcttg ctcagactta tcctatttat cacagcggag attatataaa cgataaagag
     6661 aatgatattt ttgatgttac gcttcgtaac tggggtccat gtaatcccta tgatccgatg
     6721 gacccgttca catacaataa ttctgtcaat gatttttcca ggcttcgttt ggtaggagct
     6781 cctcctttgc cgactgcttc cgatgtgaca gtctgttccg ggacacctgt ttccttatca
     6841 gcgatcagaa atggtacaaa tccgggccaa ttgcattggt acaataatgc tgacttgcat
     6901 ccgggccacc aggttggaac cggcacaact tatagtccgg gtatccttgc tgatggcacc
     6961 tataattatt gggttaggga aataggtact accgggcagt tttgtgaagg accggctaaa
     7021 caagtgacag ttacagtaag aagacttctc cagactccgc caaatatatc gggcccaaca
     7081 aatacgtgtc ctaatgtaaa agatctggtc tttagtgttc cacataatcc gcctgtgatg
     7141 tcaccgggtg ggccaacaag atatgtatgg tcggtgcccg ccggattaac aataaaatca
     7201 ggtcagggta ccagaagcat cactgttgat gtagggacaa cggtcgggaa taaaacgata
     7261 agtgtttacc ttgaatatac ttcagatcct tcatgcaatt caaatccaag caccatacaa
     7321 ttaactgtat tttcagtcaa tcacaatccc ggaacaatca gaggatcaaa tatttgttcg
     7381 ggcgaagtgg taaatatcac aaataatgct agcgcttcaa caggaaatcc ttcttccggc
     7441 ggacttacct atagctggga aagagctgcc ggaccaacct tttcagtatg gacaccatta
     7501 acaggttctt ctgcaacttt tagtgaaaca attagcactc caggagttta ccgataccgc
     7561 agaacagcaa catttaattg tggagatcct gtatctacaa ccagggatat cacagtattt
     7621 tcaataacac acaatggagg tgcaatttcc ggcacagata tttgccaggg aggaacagtc
     7681 aatataacta atcagacaag tgcgtttaca ggtacacccg catcatcagg gccaacatac
     7741 agttggcaca gggctccggg accagccttt tcgtcatgga ctcttctatc aggatcatcc
     7801 gcaacatttt cagaaattgt aaatactcca ggaacttacc gataccgcag aacagcaaca
     7861 ttcggatgtg gaaatcccag aactgctata gtggatataa ctgtcaatcc ccttcctgct
     7921 ccggttataa gtggagaaac aagtccgtgc catggtaata caaaagtgta cacaacacaa
     7981 taccatgacg ggcataatta tagttggtct gttgttaatg gaacaatatc cggaagttct
     8041 acaggaaaca gtgttactgt catttggaat ctgtcatccg gaacaggttc cttaagagtt
     8101 actgaaacaa tacaatcctc gggttgtgct gtaacaactc caaattataa tatcactatc
     8161 aatccggccg ctcccggagc agcaggcgcc attacaggtt tgtcaaacat gtgctatgct
     8221 cagaccgggg taacatattc aattctcccc gttgcaaatg catcaaatta tgtgtggagt
     8281 gttcctgctg gtgtaaatat agtctccggc caggggacca cttcactaaa ggttgatttt
     8341 acatctgctg cagtatctcc ggtcaccatt tctgtttatc cggaaaatgg ttgcggtgcc
     8401 ggcggaactt catcaagggt gataactatt tatccggaac ttgagggtgg ttcaataggc
     8461 ggcgaccaga caatatgtta cggctatgat gtggcggcat ttacaagctc cggtgctgca
     8521 acaggcgggg caggttcatt ttcttattta tggcaatata ccacagatat gtcagctcct
     8581 ttgggcagtt ctgcatggca aaatatttct tccgccacag gtctgacata tgaccatggt
     8641 actttaaccg gaacaacacg ttttgtgagg agggctgttg aaggtacatg ctcaactcct
     8701 gtttacagta atattgtaac ggttactgta aggccgcaac tgaacggtgg ctcaataggt
     8761 agcgaccaga caatttgtta cggcgctgat gtggcagcat ttacaagctc cggtgctgca
     8821 acaggcgggg caggttcatt ttcttattta tggcaatata ccacagatat gtcagctcct
     8881 ttgggaagtt ctgcatggca ggatatttct tctgccacag gtctgatata tgaccatggt
     8941 actttaaccg gaacaacacg ttttgtgagg agggctgtgg aaggtacatg ttctactcct
     9001 gtttacagta atattgtcag agttactgtt cgccctgaac tcaaaggcgg tttgatttca
     9061 ggaaaccaga caatctgtta tggtacaatt cctgctcaat ttacaaattc aacggcagca
     9121 agcggcggag caggttcgtt cacctattcc tggtggtata ctactgatgc aggtttagac
     9181 gctggtgatc ctggatggaa tgaaatttct tcaagtaata ctacaggatt taattataat
     9241 aatactctta ccacaacaac acgtttcgtc agaagagctg ttgatggtac atgtacaaca
     9301 ccggtttaca gcaatattgt aacggttact gtccggccgc aactggacgg aggagaagtc
     9361 ggcaccagtc agactgtatg ctacggcaat gatgttcccg gctttacaag taaaagtcct
     9421 gcaacaggcg gggaaggttc attcacctat acctggcagt ataccaccaa tatgctggca
     9481 actccgggtg atggaaactg gactgatatc ggatcgagca actctgtaac ctatgatcat
     9541 ggaaatctga ccactacgac taaatttgtc aggaaggcag tggaaagtac atgttcacct
     9601 gtggtttaca gtaatgaaat tattatcact gtgatgcctg ccctgaacag cggagtaata
     9661 ggcagcagtc attttatctg ctatggagaa gcacctaatc catttagcag tctggtccct
     9721 gccagcggcg gcggtggttc tttcacctat acctggcagt acacaacaaa tacatccgcc
     9781 gtggcgggtg acggcaactg gactgatata gccaattcag acgatgaagc atatacgcac
     9841 ggggttctta ctgcaactac cctgtttgta aggaaggccg aggatgcctc ctgtacaaca
     9901 ccggtctatt caaacataat aactgtaacg ataaatcctc tgccagtaac ttcattaata
     9961 aggggaccgt cggttctctg tgaggatgca acaaacaggg tttatgaagt tgataaccat
    10021 cctggttcca cctatacctg gacagtgccg tcatcaatgg ttattacatc acctcccgga
    10081 atgtatttca tcattgtgga tgctgtcccg ggaatggcgg ctccgggtga cacgataacc
    10141 gtgacggaaa caattctatc cacgggttgt gtcggaaaac ctgttaaatt accggtatcg
    10201 gtggtgccga tcataccggg tgttgttgtg aacggtccgg caagcctgtg cctgggagat
    10261 acggctgttt attccgtgcc c
//
LOCUS       DAOSPX010000080        10243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6371_length_10243_cov_4.903808, whole genome shotgun sequence.
ACCESSION   DAOSPX010000080 DAOSPX010000000
VERSION     DAOSPX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6371_length_10243_cov_4.903808"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLZ75_05630"
     CDS             complement(<1..714)
                     /locus_tag="PLZ75_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS71913.1"
                     /translation="MGFIFGHVIFILTLIIFGSYLTLGIFSAIALRKYLRKNSYVNYN
                     SLVLSPLSPKVSIIAPAFNESKTIIDNVRTLLSLYYNNFEVILVNDGSTDDTFEKVEE
                     AFELEKVNYYFDYRLPCEKIKGVYRSANPSFNRLTVIDKNNGGKADSLNAGINICKSS
                     LFVSIDADSIIEADSILKLVKPFLEEKEKKVIGTGGVIRIVNSCDTELGHIRQINVPR
                     QILPRLQVLDYTRAFLLGRM"
     gene            complement(721..2406)
                     /locus_tag="PLZ75_05635"
     CDS             complement(721..2406)
                     /locus_tag="PLZ75_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS71914.1"
                     /translation="MRYILFFILIFISAKGTVAGRSEGITDNPMASGEEFTGSGNHPD
                     SGQFIDYVINETIVRGFITSVKDQFPVQLGAFRVKFNAEAFLSEVKRHIDANAIMFRE
                     DGWYKIRISGNPIPEEALTAEAETIQSGTETVNVWSGTEGSGSKAEGGRFLIARAEYF
                     DRSDTAEAVSDTSEVMPYTAAEENDGAFMKNPPGNLLIGNNSPWLKRITYFGKSLALI
                     NALIITILFSICCMILLLIFILFNRRRMEKKQKLHQYLMEKYQELIINYLFGTATPED
                     FRPIASDTYRRQVLIDQMIDVSINLKGEEARKLLGLYKHLGLDRDSERRAHDRRWHKK
                     IKGFRELAFMNIKSANEAIYKALNSTNEILRMEAQIALVRLGDDKPFDFLNHLERPFS
                     LWEQISLHELIVQHNLPLPEFSQWLNSPNPTVAMFALRMIREFKQSEAEEAVHGILMH
                     PDPEVKKLAIQVAGDMNMRSTLETMKHMYKFQDYDICLEIIRSMGKMPDLSLLGFLKL
                     VLDKEDDVQLQIEATKAIENNGEEGVRALVKIMKSEYKNYNIIVRHVLDRRIY"
     gene            complement(2406..3476)
                     /locus_tag="PLZ75_05640"
     CDS             complement(2406..3476)
                     /locus_tag="PLZ75_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HOS71915.1"
                     /translation="MNRSFILFTAILLSAGALVCRPAYAQEAGDPESEYMRIRSLAFG
                     GDYASAAAAARKLVNVSPSYGDARILLGRILAWQKKYDEAAAVIDTLLATDPDNADAL
                     AARRDISLWSRENSPVATDIRAGYSFDSFTEPYRRFWQVFRAGAGHRFDWGPAAAGLN
                     LGTITMNDTLVAELQFEAEAWPKLSDKNYAYLSYAFSPGKYFPGHRAAAEVWQILPKG
                     WAMSAGMNYYRFDRNIFIAGLSAEKYIPGYWFSLKGLLAFRNEAARPAVYLNARKYFN
                     DTDYLQLTLGTGAAPDEPVYIQTNIERLSANSIRLAYYGFLTKKLSIRVGTGYSREEF
                     RGGNWRNRLEGHVNLFYAIKMK"
     gene            complement(3480..3875)
                     /locus_tag="PLZ75_05645"
     CDS             complement(3480..3875)
                     /locus_tag="PLZ75_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS71916.1"
                     /translation="MAKIQRCLMNNSMRILICEDNRLALKTISVVLEKEGFKSETAEN
                     GADAISLLKNNQYDMVIVDIHLPYHSGLELVQYLRSDLKLQTPVLIVTAFSDPQVRRR
                     ASELGIDGYILKPFSPSDLISTIRTILKN"
     gene            complement(3991..6669)
                     /locus_tag="PLZ75_05650"
     CDS             complement(3991..6669)
                     /locus_tag="PLZ75_05650"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOS71917.1"
                     /translation="MKSSVRQTFLYLIISILIPAGPARAASPEPGNRPSTQDSLKQEI
                     RQPVYTTSRLVTPKPVIDGKLDDECWKHGTWAGDYRQYIPHEGAKPTWPTEMNIQYDD
                     RNLYIAFRAWDGEPDKIVRMSGGRDEIVGDVVGIAFDSYRDYRTGFEFTITAWGQKVD
                     LVLFNPMDWDFNWNAVWKGKTGLEDSAWVAEIEIPFSQLRYSNKDEQVWGMHTWRWIA
                     RLQEESNWEIQSKTGPGLLYNFGELKGIKGLPRSRRLEIMPFVLGELETMKAEAGNPF
                     NKNGRIWKGNAGLDAKIGISSNFTIDLTVNPDFGQVESDPSVMNLTAFETFYEEKRPF
                     FLEGLTIFNYDFDDKNIFYSRRIGQAPSLVISPDGDSFVSIPDKTRILSAAKFSGTTA
                     EGLSLGLIQSVTATEYARIGDRHGNITKSRIEPLTSYTVARVQKGYNAGNTTVGAMLT
                     STNRDVRGDAPGFPARNAFTGGFDLRHHWKDKKYFLETKLLGSHISGNTSAVRELQRS
                     PARYYQRPGADYVIYDTARTSLSGFGGKIRAGKGSKGFWKYSTGISWLSPGLELNDIG
                     YMNKSDEIRHQTDLEYIVNKPVSIFHTWSLNLEQFNSWNFGGTYLGSGANLKALAEFT
                     NNYRISTNLLFHSKAADPWKLRGGPDMIMPGRLMVSGKAGTDPSKRYMFELRYNYEMS
                     GNKSSRGFVIGPGITLRPLDFLKIGMTVDYDKNHDELQYIATKTIDERAGDRRYILGT
                     IDQKTVALTFRADLNLTPEFSVQYYGSPFVSRGAYSALKRVRNAGAGKYADRFGLYDD
                     PVLSGGNYILSDYDSGERKEYSLADPDFNFYQFRSNLVAKWEYRPGSWIYLVWSADKT
                     GRDNLSGTSLPESYRILKNVYPENIFLIKINYWFSL"
     gene            complement(6725..7168)
                     /locus_tag="PLZ75_05655"
     CDS             complement(6725..7168)
                     /locus_tag="PLZ75_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71918.1"
                     /translation="MLCKLLLLAVFGLAMAHFEGVVVFYLRKVLGLNDTVSNRESVRE
                     FPKRYLYIEMTREAATIIMLVAIAILAGSTWIERGILFLWTFAFWDLFYYLSLYILTG
                     WPENLKATDVLFLIPVPWIAPVWLPVGISSATIIVIALLFLFNII"
     gene            complement(7349..8047)
                     /locus_tag="PLZ75_05660"
     CDS             complement(7349..8047)
                     /locus_tag="PLZ75_05660"
                     /inference="COORDINATES: protein motif:HMM:NF027590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HOS71919.1"
                     /translation="MNTLADKIISFYTEIDFRGILPAGVSIMNPFRNNPDVINTVTLF
                     YRKYYSDYNKRHMIIGINPGRLGAGATGIPFTDTIRLKQVLGLPAPGIETYETSSVFM
                     YGMIDRYGGPEAFYGDHYISSVSPLGFTVTGRNGRQVNYNYYDSRELTGHIMDFILDS
                     LRAQLEFGIFRDTCFCLGSGKNYSFLTELNNTYGFFEKIVPLEHPRYIMQYRLKKKQF
                     YTDMYVEKLKAPLQ"
     gene            8173..8643
                     /locus_tag="PLZ75_05665"
     CDS             8173..8643
                     /locus_tag="PLZ75_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOS71920.1"
                     /translation="MANNDSIRPYGDKQPVFDKRYLEMARIWAENSYCRRRKVGALIV
                     KGRMIISDGYNGTPAGFENVCEDENNVTKAYVLHAEANAITKVAKSNNSSEDSTLYVT
                     TSPCIECAKLIIQSGIRRVVYCNRYRITDGLDLLKRAGIDLVYIDPENDIYEQQ"
     gene            8630..>10243
                     /locus_tag="PLZ75_05670"
     CDS             8630..>10243
                     /locus_tag="PLZ75_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOS71921.1"
                     /translation="MSNNNNRWNIFLPLMVAIAVVLGILVGIILPRKNDSAAQLPGIR
                     PRNDKLSTILNIIERNYVDSVNRDNLVEAAIPAILKKLDPHSVYIPAKDLARANESLQ
                     GNFEGIGISFNMLTDTIIVISTIPGGPSAKLGLLAGDRILYVNDSLVAGKGISDEDVM
                     GMLKGPKGTTVRVKILRKGYNDLLTFNIIRDKIPIYSVDVAYMVNDNTGYIKINNFAV
                     TTFDEFMKGLKELREKGMTKLIVDLRGNAGGIMEDAVQIANQFLKEGQLIVYTMGRNQ
                     PRNEARATGKGEFENGDLVILIDEWSASASEILAGAVQDNDRGTIIGRRSFGKGLVQE
                     PIPFADGSGMRLTIARYYTPTGRSIQKPYTEGFTDYFNDLSSRYSRGEFEVSDSIHFS
                     ESLRFTTPGGRTVYGGGGIMPDEFVPVDTSEISSYFLMVRPFIYQFALKYTENNRNAL
                     EKFTEAAGLEKYLDRQGLLQQFTRFMASGNMRSGQSAAAGAGTGKNGDAAEDNGRNSG
                     GTVIKPDPEGLKVSGRVIHTQIKAYIARNI"
BASE COUNT         2615 a         2362 c         2443 g         2823 t
ORIGIN      
        1 cattctgccc aggagaaatg cacgggtgta atcgagaacc tgcagacgtg gcagtatctg
       61 cctgggaaca ttgatctgac gtatatggcc cagttcggtg tcgcatgaat tgacaatcct
      121 gatcacccct cccgtgccta tgaccttttt ttccttttct tcaaggaagg gttttacgag
      181 tttgagtatc gaatcagctt caattattga atcggcatct atggagacaa aaagggaact
      241 tttgcatatg tttatccctg cgttcaggga atctgccttc cctccgttat tcttgtcaat
      301 cacagtaagc ctgttgaagg agggattcgc ggatctgtaa acacccttta tcttttcgca
      361 gggaaggcgg taatcgaaat agtaattcac cttctcgagc tcaaaggctt cctctacctt
      421 ttcaaaggta tcatcagttg atccgtcatt tacgagtatc acctcaaaat tgttgtaata
      481 gagggagagg agagttctga cgttatcaat tatcgttttg ctttcattga aagcaggagc
      541 gataattgac acttttggac tcaagggtga cagaacaagt gaattgtagt tcacatagct
      601 gtttttcctg agatatttcc tcagtgcgat tgcggagaat atgcccagtg tgagatacga
      661 cccgaagatt atcagggtaa gtatgaatat tacgtgtccg aaaataaaac ccatagccag
      721 ctagtatatt cttctgtcaa gcacatgcct gacgattata ttataattct tgtattccga
      781 cttcattatc ttgaccagtg ccctgacgcc ttcctcgcca ttgttttcaa tagcctttgt
      841 ggcttcaatc tgcagctgaa catcatcttc cttgtcaaga accagtttca ggaatccgag
      901 cagtgacagg tcaggcattt ttcccattga tctgattatt tcaaggcata tatcatagtc
      961 ctggaacttg tacatatgtt tcatggtttc cagtgttgaa cgcatgttca tgtcgcccgc
     1021 cacctgtatg gcaagctttt ttacctcagg atcgggatgc atcagaatgc cgtgaaccgc
     1081 ttcctctgct tcagattgtt tgaattccct tatcatcctg agggcaaaca ttgcaaccgt
     1141 cggattcggg gaattgagcc actggctgaa ttccgggaga ggcaggttgt gctgcactat
     1201 aagctcatga agcgagatct gctcccagag cgaaaacggc ctttcgagat gattgagaaa
     1261 atcaaagggc ttgtcatcac ccagccgtac cagggcaatc tgggcttcca tcctgagtat
     1321 ttcatttgtg gaattgagag ccttgtatat tgcctcgttt gcacttttga tattcatgaa
     1381 tgccagttcc cggaatccct tgatcttctt atgccatctg cggtcgtgtg ccctcctttc
     1441 agaatccctg tccagtccca ggtgcttata cagcccgaga agctttctgg cttcttctcc
     1501 cttcaggttg atgctgacgt caatcatctg gtcaatcagt acctgcctgc gataggtgtc
     1561 ggaggcaatc gggcggaaat cttccggagt ggcggtgccg aaaaggtagt tgatgatgag
     1621 ttcctggtat ttttccatga gatactggtg aagcttctgc ttcttttcca tccttctcct
     1681 gttgaagaga atgaagatca gaaggagtat catgcaacag atactgaaca gtattgtaat
     1741 tatcagcgca tttatcagtg ccagggattt cccgaaataa gttattcttt tcagccaggg
     1801 actgttattc ccaatcagaa gattgccggg aggatttttc atgaaggccc catcgttttc
     1861 ttctgctgcg gtgtatggca ttacttccga agtatcagat acagcctcag cagtatctga
     1921 acgatcaaaa tattcagccc tggcaatcag gaatcttccc ccttcagctt tgctgccgct
     1981 gccttcggtc cctgaccaga catttacggt ttcagtcccg gactgtatgg tttctgcttc
     2041 tgcggttaag gcttcttccg gtatggggtt cccggagatc cggatcttat accagccatc
     2101 ctccctgaac atgatggcat tggcgtcaat atgccttttt acctcactca aaaaggcctc
     2161 agcattgaat ttcaccctga aggcgccgag ctgaacagga aactgatctt taaccgaagt
     2221 aatgaatccc cttacaattg tttcgttaat gacatagtca atgaactgac cgctgtccgg
     2281 atgatttccg cttccggtaa attcttcccc ggatgccatg ggattgtcag tgatcccttc
     2341 ggaccggcct gcaacagttc cttttgctga aataaatatt aatatgaaaa aaagaatata
     2401 tctcattatt tcatttttat cgcgtaaaaa agatttacat ggccttccag cctgttccgc
     2461 cagttgcccc cgcggaattc ttcacgtgaa tagcctgttc ccacgcggat tgacagtttt
     2521 tttgtcagga agccataata ggccagccgg atgctgtttg cacttaaccg ttcaatgttt
     2581 gtctgtatgt aaacaggctc atcgggtgca gcaccggttc cgagtgtcag ctggaggtag
     2641 tcggtatcgt tgaaatattt ccttgcattg agatataccg ccggtcttgc cgcttcattc
     2701 ctgaaagcca gcagaccctt aagtgagaac cagtatccgg gaatatattt ttcggcggat
     2761 aatcctgcaa tgaatatatt cctgtcgaag cggtagtagt tcatacctgc tgacattgcc
     2821 cagcctttcg ggagtatctg ccacacttcg gccgctgccc tgtggccggg aaaatatttt
     2881 cccggactga aggcatatga gaggtaagca taatttttat ctgacagttt tggccaggct
     2941 tcggcttcaa actgcagctc ggccaccagt gtgtcgttca tggttattgt acccaggttg
     3001 agtcctgctg ctgcaggtcc ccagtcaaaa cggtgaccgg cccccgccct gaatacctgc
     3061 cagaatcgcc tgtagggctc ggtaaagctg tcaaagctgt agccggccct gatatcggta
     3121 gccaccgggg agttttccct cgaccagagt gaaatatccc tccgggcagc aagagcgtct
     3181 gcattatcag gatccgttgc cagaagggta tcgattacgg cagctgcctc gtcatatttt
     3241 ttctgccatg caagtattct tccgagaagt atccgggcat cgccgtatga aggactgaca
     3301 ttgacaagtt tcctggcagc ggccgcggca gaggcataat cgcctccgaa ggcaagggat
     3361 ctgattctca tgtattcgga ttcaggatca cccgcctcct gggcgtatgc cggtctgcag
     3421 accagtgctc cggctgataa caatatggcg gtaaacagaa tgaaggatct gttcataaat
     3481 caattcttta atatggtccg tattgtgctg atcagatcag aaggactgaa tggttttagt
     3541 atatatccgt caattcccag ttcagatgcc cgtcgccgga cctggggatc actgaatgcc
     3601 gttactatca gaacaggagt ctgaagcttc aggtccgaac gcaggtattg caccagttcc
     3661 agtccgctgt gatagggcag gtgaatatcc acaatcacca tatcatactg gttgttcttc
     3721 agcaatgaaa tggcatcagc accattttct gcggtttcag acttaaaccc ttctttttca
     3781 agaacaacag aaatagtttt aagcgccagc ctgttgtctt cgcaaataag tatcctcatg
     3841 ctgttgttca taaggcatct ctgaatttta gccattccta tgccttatac gttgtaaaaa
     3901 tgaaaattat acacagttat accaaaattc cctgaaaaat tttgctaaca atttcaggaa
     3961 atggattaac aaccgtattt tttagacgaa ttacaatgaa aaccagtagt tgatcttgat
     4021 caggaaaata ttttcaggat aaacgttttt cagtatcctg tatgattccg gaagggatgt
     4081 acccgataag ttgtcccgtc ctgttttatc agctgaccat acaagataga tccatgatcc
     4141 gggccggtat tcccatttgg caaccaggtt tgagcggaac tggtaaaagt tgaaatcggg
     4201 atcggcaagg ctgtattctt ttctttcacc actgtcgtaa tcagaaagaa tataatttcc
     4261 gccgctcaga acgggatcat catataatcc gaacctgtcg gcatattttc cggccccggc
     4321 attccttacg cgtttcagag cggaataggc gcctcttgag acgaagggac tgccgtaata
     4381 ctgaaccgag aattcaggtg taagattcag atcggccctg aaggtgagcg ccactgtctt
     4441 ctggtcgatg gttccgagta tgtatctcct gtcacctgcc cgttcatcaa tggtttttgt
     4501 ggcaatatac tggagttcat catgattctt gtcataatcg actgtcattc cgatttttaa
     4561 aaagtccagc ggacgtaagg ttattccggg tccgatcaca aaaccccggg aggatttgtt
     4621 tcccgacatt tcatagttat acctgagttc aaacatgtat ctttttgagg gatcggttcc
     4681 ggcttttccg gaaaccataa gccgccccgg cattatcatg tcgggtcctc ccctgagctt
     4741 ccaggggtct gcggcttttg aatgaaaaag gagattggtg ctgatcctgt agttgttggt
     4801 aaattcggct aatgccttaa ggttggcccc tgaccccagg taagtgcctc cgaaattcca
     4861 cgaattaaat tgttcaaggt tcaggctcca ggtatggaat attgaaaccg gtttgtttac
     4921 aatgtattca agatcagtct ggtgacggat ctcatccgat ttgttcatat agccgatatc
     4981 attcagttca agtccgggtg acagccagct tattccggta ctgtatttcc agaaaccttt
     5041 tgatcccttt cctgccctga tctttccgcc aaagccgctc agggatgttc ttgcagtgtc
     5101 ataaatgacg taatcagcac caggccgctg gtaatagcgt gccggtgagc gctgcagttc
     5161 ccttacagca cttgtgttac cgctgatgtg actcccgagc agctttgtct cgaggaaata
     5221 tttcttgtct ttccagtggt ggcggagatc aaaacccccg gtgaaggcat tcctggcagg
     5281 gaagcccgga gcatctcccc ttacatcccg gttggtggaa gtaagcatag cgccaacagt
     5341 ggtatttccg gcattatatc ccttttgcac acgggcaacg gtatagcttg taaggggttc
     5401 aatccggctt ttggtaatat tcccgtggcg gtcaccgatc ctcgcatatt ccgtagccgt
     5461 aacactctgg atcaggccca gcgacaatcc ctctgccgtg gtaccgctga acttggcggc
     5521 actcagtatt ctggtcttgt cgggaatgct tacaaaggaa tcgccgtcag gactgatgac
     5581 cagtgaagga gcctggccta tgcgcctgga atagaaaatg tttttatcgt caaaatcgta
     5641 gttgaatatg gtaagtccct ccaggaagaa gggacgtttt tcttcataga aggtctcaaa
     5701 ggcggtaagg ttcattaccg acggatcgga ttcaacctgg ccgaaatcag gattcaccgt
     5761 aaggtcaatg gtgaaattgc ttgagatgcc tattttagca tccagtccgg catttccttt
     5821 ccagattctg ccatttttgt taaagggatt gcccgcttcg gccttcattg tttcaagttc
     5881 tccaagtaca aagggcatta tttcgaggcg cctggatcgt ggcagaccct ttatcccttt
     5941 cagttcacca aaattgtaaa gcaatcccgg accggttttg gattgtattt cccagttgct
     6001 ttcttcctgg agccgcgcta tccacctcca tgtatgcatt ccccatacct gttcatcctt
     6061 gttgctgtaa cgcagctgac tgaagggaat ctcaatttct gctacccatg cggaatcctc
     6121 cagtccggtt ttccccttcc atactgcatt ccagttaaaa tcccagtcca tgggattgaa
     6181 aagtacaaga tcgaccttct gtccccatgc tgttatggta aattcaaatc ctgtacggta
     6241 atccctgtaa ctgtcaaagg ctattccaac cacgtccccg acgatttcat cacgtccccc
     6301 ggacatcctg actattttat ccggctcacc gtcccaggca cgaaaagcta tgtagagatt
     6361 ccggtcgtca tactgtatgt tcatctccgt gggccaggtg ggttttgccc cttcatgggg
     6421 aatatactgg cgatagtctc ctgcccaggt tccgtgcttc cagcactcat catccagttt
     6481 gccgtcaata acaggtttcg gagtcacgag gcgggaagtg gtgtaaacag gctgcctgat
     6541 ttcctgtttc aggctgtcct gtgttgacgg cctgtttccg ggttcaggcg aagcggccct
     6601 ggcaggaccg gccggtataa ggatggaaat gatcaggtaa aggaatgttt gtctgactga
     6661 cgatttcata cgggagtcat taaaaggttc cgggaaagtg tttctgcagg gaaatgccgg
     6721 caggtcatat aatattgaac aggaataaca atgctattac aattattgtt gcggaagaaa
     6781 tacccaccgg cagccatacg ggggctatcc acggtacggg aatcaggaaa aggacatcgg
     6841 ttgctttcag gttctccggc catcctgtca gaatatagag ggaaagatag taaaacagat
     6901 cccagaatgc aaatgtccag agaaacaata tgccgcgctc aatccatgtt gatccggcaa
     6961 gtatggcaat tgccaccagc attatgatgg tagctgcttc cctcgtcatt tcgatataca
     7021 aataacgttt cgggaattcc cttaccgatt ccctgttcga gactgtatcg tttaaaccga
     7081 ggactttcct tagataaaac acaacaaccc cttcaaaatg agccattgcc agtccgaaaa
     7141 ccgccagaag aagtaatttg cacagcatgg cagcagattt ttattattct tacaatttat
     7201 atgaaaatac cggaataaag ctctgccaga tgatgatttt ttgaaattga tccggatccg
     7261 cttcaggaaa ttaacatttc acctgactga cgggcagact gtaatccgga aagggctgtc
     7321 aggcaggcag gactccgggc ctgcggaatt attgcagagg ggccttcagt ttttccacat
     7381 acatatctgt ataaaactgt ttcttcttca gacggtactg catgatatac ctgggatgtt
     7441 caaggggaac tattttctca aagaaaccgt atgtgttgtt tagttcagtc aggaagctgt
     7501 agtttttacc gcttcccaga caaaaacatg tatccctgaa gattccgaat tcaagctggg
     7561 ccctgaggga atcaagtatg aaatccatta tatggccggt cagttccctg ctgtcgtagt
     7621 aattgtaatt cacctgcctt ccgttgcgcc cggtaacggt gaatcccagg ggactgacgg
     7681 aacttatata atgatccccg tagaaggctt caggtcctcc atagcggtca atcattccgt
     7741 acatgaaaac cgatgatgtt tcataggttt caattccggg agccggaagc cctaatacct
     7801 gtttgaggcg aatggtgtcg gtgaatggta tcccggttgc tcccgcgccc agccttccgg
     7861 ggtttatccc gattatcatg tgccgtttat tgtaatcgct gtagtatttt ctgtaaaaga
     7921 gggttactgt attgattaca tcgggattgt ttctgaaagg gttcattatt gagacaccgg
     7981 cgggaaggat tcccctgaaa tctatttcag tgtaaaagga gataattttg tctgcaagtg
     8041 tattcatgaa acaaaaatag gaatgacggg tttaaatgat gttgatatta aagttaaaaa
     8101 ttttaataac atgctaaata aacgtatttt aatgattaat tttgctggat tctgatagca
     8161 gggcattaca aaatggcaaa caacgattcc atcagacctt atggtgataa gcagccggtt
     8221 ttcgataagc gttacctcga aatggcccgt atatgggcag agaactccta ttgccggcgg
     8281 cgcaaggtgg gagcccttat tgtaaaggga aggatgatca tttcggatgg atacaacggt
     8341 acccctgcag gttttgagaa tgtatgcgag gatgaaaaca atgttactaa ggcatatgtc
     8401 ctgcatgcag aggcaaatgc cattacaaaa gtggcaaaat caaataattc cagtgaagat
     8461 tcaacgctgt acgttacaac ctcaccctgc attgaatgtg caaaactgat aatacagtcg
     8521 ggcatcagaa gggttgtgta ctgtaacaga tacaggatca ctgacgggct ggacctttta
     8581 aaacgtgccg gaattgatct ggtttatatt gatcccgaaa acgatattta tgagcaacaa
     8641 taacaacaga tggaacattt ttctgccgct gatggtggcg attgcagttg tactgggcat
     8701 acttgtcgga attatccttc cccggaaaaa tgattctgca gcgcagctgc ccggtatccg
     8761 gccgagaaat gacaagctga gtaccatact gaatattatt gaaaggaatt atgtggattc
     8821 cgtaaaccgc gacaatcttg ttgaggcagc cattcctgcg attctgaaaa aacttgatcc
     8881 tcattccgtt tacataccgg caaaagatct tgcccgggca aatgaatccc ttcagggaaa
     8941 ttttgaagga atagggatat cctttaacat gcttaccgat acaatcattg ttataagcac
     9001 tattcccggg gggccctcgg caaagctcgg actgcttgcc ggtgacagga tactttatgt
     9061 aaacgattca ctggtggctg gaaagggaat aagcgatgag gacgtgatgg ggatgctgaa
     9121 ggggccgaaa ggtaccacgg taagggtgaa gatactccgg aagggctaca atgatcttct
     9181 tacattcaat ataatacggg ataagattcc catttacagc gtggatgttg cctacatggt
     9241 caacgacaac acgggatata tcaagataaa caattttgca gtcaccacct tcgatgaatt
     9301 catgaagggt ctgaaggagc tgagagagaa gggtatgacc aagcttatcg tggatctgcg
     9361 cgggaatgcc ggggggatta tggaagatgc cgttcagatt gccaaccagt tcctgaagga
     9421 aggtcagctt attgtttaca cgatgggaag gaaccagccg aggaatgagg ccagggccac
     9481 gggcaagggt gagtttgaaa acggcgacct tgtcattctg atagatgaat ggagtgcatc
     9541 agccagcgag atccttgcag gggcggttca ggacaatgac cgggggacga ttatcggaag
     9601 aaggtcattc gggaagggcc ttgtacagga gccgataccc tttgccgacg gctcaggcat
     9661 gaggcttacc atagccaggt attacactcc tacggggcgg tcaatacaga aaccctacac
     9721 tgaaggtttt acggattatt tcaatgatct aagctcacgc tacagccgcg gggaattcga
     9781 agtatccgac agtattcatt tttccgaatc cctccggttc acgacacccg ggggaagaac
     9841 ggtctatggc ggaggcggga tcatgcctga cgaatttgta ccggttgaca cttccgaaat
     9901 ttcatcgtat ttcctgatgg taaggccctt catctaccag tttgccttaa aatataccga
     9961 gaacaaccgg aatgcactgg aaaaattcac tgaggcagct ggactggaaa aatatctcga
    10021 caggcagggt ctgctgcagc agttcaccag gtttatggca tcaggcaaca tgaggtccgg
    10081 acagtctgcg gcagccggtg ccggcaccgg gaaaaacggg gatgcggcag aggataatgg
    10141 aagaaattcg ggaggcaccg tcattaaacc tgatccggaa ggactgaagg tttcgggaag
    10201 ggtgatacac acgcagataa aggcttatat cgcacgcaat atc
//
LOCUS       DAOSPX010000081        10171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6415_length_10171_cov_4.465500, whole genome shotgun sequence.
ACCESSION   DAOSPX010000081 DAOSPX010000000
VERSION     DAOSPX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6415_length_10171_cov_4.465500"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1865)
                     /locus_tag="PLZ75_05675"
     CDS             complement(84..1865)
                     /locus_tag="PLZ75_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS71922.1"
                     /translation="MASRKIITCCFMFLALFASCNQGQYHKLIEAESFDDKGGWVVDP
                     QFVEQMGSPYLLAHGLGIPVENARQAIDLPSGGRYHIWARTKNWAPGDWEAPGRFQVI
                     INGKKLKTILGTAEGWGWQYAGSIRVKDLHCLVELQDLSGFGARCDAVYINSGKISPP
                     SDPDELLRWRRKMLSESDTPPESESFDLVITGGGIAGCAAAIAAAEQGLKVALIHDRP
                     VLGGNAISEIRVHTEGITWKAARILNMLNTVWWPNGSPDAALDDKKRHDNMAKYPDIS
                     VFLNWRAYTTNVTDDSISSVDARHTSTGETMRFMAPLFVDCTGDGWIGYWAGAEYMYG
                     REDSSEYNENWEIHGELWSPAEADGRVMGSSVLWRTVDTGRSVAFPEVPWATAVAGDY
                     SATSGTWKWEFSRNDLHQVDDAEEIRDHMHRAVYGSFSNAKKDPANANLALEWVSWLV
                     GKRESRRLTGDYIYTFDDEKTMREFPDAVAMEERDIDVHYQQNLKDPSQPDFLSEALF
                     YKVDHYYIPYRTLYSRNIKNLFMAGRCFSCSHVGLGGPRVMHTTGQMGVAVGYAAALC
                     KKYNTGPRGVYLNHIGELKKLIDDCSK"
     gene            2307..4244
                     /locus_tag="PLZ75_05680"
     CDS             2307..4244
                     /locus_tag="PLZ75_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS71923.1"
                     /translation="MRTSVMFFLIIYIFGSCTPSKHDSGGPEWAVSVIPSSVRLDPVS
                     NEIIDNRFVRLKGNKNLNRDVLKKNSIYDGKAVSLHAARGEYISFQLVLTSLTKETLD
                     NIRVTMEPFRKGEKEFKAGPELFLEWAVEVKTPSTGYPEASLGNGWYPDALIPFSYIQ
                     DDSSGVKWRWTYPLWLPDFNNRIDNQRSLLVWIDQYIPFPRDEAGPGIYSSELAVTIG
                     GFTRKIPVNLTVWDFALPNENKFKAGLQHEGFMKNMTERMELEIYQLLKRNRISMLDP
                     TYEPGLKIGSNGNIAIDWKALDERLGKYFTGKAFTAEYGYSYGPGYGEPIETFVLPFD
                     VSGKHGTKGWPDIGGTDVERNPSNRAIYTECIRKVRDHYKTIIDPEKTDITVYLNGLD
                     ESYFPEAWSRMVYYGNIFREYYPEAGFRIDGGYSEEALEIVKDSITSWASHTIEYDFE
                     TVKKYQDMGIKIWLYGPLLYESKINSWVGSSTFLDLPLVNDRAISWSSWKYRTYSWLS
                     WGAGAGWVNGWYDPESWKDASKARPETEAEFTYKKINGNALLIYAPGVVPNVSGPCPS
                     VRLKAMRDGVQEYEYLRLLSEADGNTARADSIVNEIIREPFGPKSIGNLDVWSFDAGK
                     WDAQRIAMGRMIEKKLQIRNK"
     gene            complement(4383..6212)
                     /locus_tag="PLZ75_05685"
     CDS             complement(4383..6212)
                     /locus_tag="PLZ75_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS71924.1"
                     /translation="MILIPENFQSAAGSGDPAAIFPGFYPFCLCFVLFAVLSSGTALL
                     AQSPGNNDGIKVWAVPGEQKVRPDDRPEAANLVWSAADKQISIAGAGNEHVPFQVVIS
                     APVPPGWRPEAPGGFFVKASGLKSARGDVIDQKNVKLFLEHYIQIYAASGPVGATGYW
                     PDALAPLSEPFSMQAQYIVVGNRPVWIDVFISSGTPKGIYRGTITVERFGRVIETIGV
                     SLEVYGFSLPDETHIITYMNISKNEITRFYNLHGSEPASEELLRNYFDFLFDHRMETW
                     FNDPLYPEIILKGEKVHLKFNEERYDYYMNKLKLKRVILETLPHEISRQLKADPFSPV
                     FNRVVKSYLTSVESFFRERGWLGRLVFNSPIDEPNTKKEYEETRLYAQLVHEAVPGVP
                     FLATESPVSDDPDWGTLRGYVNNFCVHGNALNDPEVKQALLEEQAKGGEATWYISCDQ
                     AYPQPNYFIDAPALDPLMVPWITERYKMNGFLYWAANFWNQTPDPWLDPVTFISGFEC
                     SGGYVLNGEGSLIYPGNHTRRYTGQPDVNGPVSSIRFELLREGIEDYEYIWMLKEKGD
                     PAFANEIVRNMVIDVSTFSRNLAELYLSRKAMAKRIEELVNQH"
     gene            complement(6212..7669)
                     /locus_tag="PLZ75_05690"
     CDS             complement(6212..7669)
                     /locus_tag="PLZ75_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS71925.1"
                     /translation="MKKKFSSILTISILSAAILVFASGCEQLLIEKPTDFVGPDNFFT
                     TPSQVESAFTASMDVLFGGWTFYDWYGYPEIFEMDDQVWGGDLVIGDDFGSGCWQKHF
                     RAIAFINPAIKALNEGQVTGISQEKADELMAQAKFLRGWNFFMLVRYYGGVPLIDENT
                     DATRVDIPRASVAEVYELIENDLLFATQHLPVKWGEENRARPAADAAKTLLAKVYITM
                     AGFPLNEPSNYVKARDMAKQVIDAGNYWLVPDVEDVFKMENTYGPEFMWCFHNSEEEY
                     VIEPQIWLPGDLAGGWGDTRTEKRWGEAFPEEPRKHAYLLLEDPWFGTGTTYLEWDGS
                     TPYVRKYLYDTPENMEKNVSYQSMPILRYADALLLFAEAENQVNGPTQAACDAVNQIV
                     NRATGGVPNPDDPLFTTSMTKEEFDEAIIQQRNWELCFEYDRWFDIQRKRILDKVTDP
                     DFQQNYSVEDYLLPIPQKDLRLNKALTQNPGYTSP"
     gene            complement(7690..>10171)
                     /locus_tag="PLZ75_05695"
     CDS             complement(7690..>10171)
                     /locus_tag="PLZ75_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS71926.1"
                     /translation="VLKDASSTAIYGSRGSNGVILITTKLGKTGKPVIDGGYSVGIQN
                     LARKLPVMNAYDFARYNNFQNKFNTAYGQTPKLYFTDDDIEYYKTHSTDWQDEIYKTG
                     IMQTSNLAISGATERLSYMVSTGYLDHKGILLNSAYNRLSLRTNLAADITDWVDFGLN
                     YSYTFEKYKSPPFEANLFTSVNDAPRWAPTEPVYDENGNYWKHRSGYGAYDTWNPVAT
                     ALETKIDNPTYQNNLNVFLNFQLLKGLTLKVMGGGTFTSSYDLKYENTKTRNGFAMNG
                     VGRINDDFRNSYQNTNILTYDQTFGIHHITITGVTEEIFSRSSGSSMRGQNFLVDQLG
                     TDNMDGAKSVTVDSWASKRTLISFMGRINYGLLDKYLLTLSYRADGSSVFGLNNKWGY
                     FPSGSVAWRISQEEFLRDSEIISDLKLRVSYGVTGNQAISPFGSLARLGSGFRYPYNN
                     GDNLNIGFGLSDIANPSLKWESTGQANIGIDLSMFGGRLTSTIDVYRKVTKDLLMPRQ
                     LPDYVGVSSVIDNIGSIENKGIEILISGDPVSGRFRWNTSVNFTLNRNKVLDLGPGVT
                     RIGYTPTSGGYSLGTEYMFLEVGQPFGQMRGWKYLGLWRLEEEAEARSYGQLPGTPKY
                     ADISGPDGIPDGQVDSFDRTTIGYGYPDFTVGWTNMFTYKGLELSFLINGSYGNQLFN
                     TLRIRRESNWEGTDPKVWNYWTPENQDTDVPALYDGQWVLDQHLVNKYIFGGGSGATS
                     RWVEDASFIRLKTLTLAYNFDSNLLRKIKFSKARLYFTGTNLLTLTRYTGYDPEVAQY
                     AYNDALIGVDQSVYPPARMFTFGIDFTF"
BASE COUNT         2594 a         2437 c         2336 g         2804 t
ORIGIN      
        1 agatgttggt aactccctgt atttattaac aatttgagtt taaagacaga cactagttaa
       61 tgacctgtca tttgatgagg ggattatttg ctgcagtcat caatcagttt tttcagttca
      121 ccgatatgat tcaggtacac tccgcgcgga ccggtattgt attttttgca cagggctgcc
      181 gcatatccga cggccactcc catctgaccg gtggtatgca ttacccgcgg gcctcccagt
      241 cctacatgtg aacagctgaa gcatcttccc gccatgaaca ggttctttat attccttgaa
      301 tagagggtgc ggtaggggat gtaataatga tccaccttgt agaacagggc ctcggaaagg
      361 aaatccggct gcgagggatc tttcaggttc tgctggtaat ggacatcaat atctctttct
      421 tccattgcaa cggcatcggg aaattccctc atggtctttt catcatcaaa ggtatagatg
      481 taatcccctg taagccttct ggattccctt ttgcccacaa gccacgacac ccattcaagt
      541 gcaagatttg catttgccgg atccttcttt gcattggaga acgatccgta aacagccctg
      601 tgcatgtgat cgcggatttc ctcggcatca tccacctggt gaaggtcatt ccgtgaaaac
      661 tcccacttcc aggtaccgga agtggctgaa taatccccgg caacagctgt tgcccacgga
      721 acttcgggaa aagcaacaga ccggccggta tcaacggtcc tccagagaac agaggagccc
      781 ataacccttc cgtcggcttc agccggactc cacagctccc cgtggatttc ccagttctca
      841 ttgtattccg aggaatcctc ccttccgtac atgtattccg ctcccgccca gtatccgatc
      901 catccgtcgc cggtgcagtc gacgaacagc ggggccatga acctcatcgt ctcccctgtg
      961 gatgtatgtc ttgcatcaac cgagctgatt gaatcatctg tcacattcgt ggtatatgct
     1021 ctccagttca gaaatacgga gatatccggg tattttgcca tgttgtcgtg acgttttttg
     1081 tcgtccaggg cggcatcggg agaaccattc ggccaccata ccgtattgag catgtttaat
     1141 attctggcgg ctttccaggt gattccttcc gtgtgaaccc gtatttcgct gatggcattt
     1201 ccgccgagca ccggacggtc atgaatcagc gccactttca gcccctgttc ggcagctgcg
     1261 attgctgccg cacatcctgc tatgcctcct cctgttatca caagatcaaa ggattccgat
     1321 tccgggggcg tatcgctttc actgagcatt ttccttctcc acctgaggag ttcatcggga
     1381 tctgacggcg gggaaatttt tccggaattg atataaactg catcgcacct ggctccgaaa
     1441 ccgctcagat cctgcagttc aaccaggcag tgaagatctt ttacccttat gcttcccgca
     1501 tactgccagc cccagccttc agcggttccc agtattgttt tcagcttctt tccgttgatt
     1561 atgacttgaa acctgccggg agcttcccag tctcccggag cccagttttt tgttcttgcc
     1621 caaatatgat atcttcctcc cgaagggaga tcgattgcct gccttgcatt ttcaaccgga
     1681 attcccagtc catgtgccag cagatatggc gatcccatct gttcgacaaa ctgcggatcg
     1741 acaacccagc cgcctttatc atcaaagctt tctgcctcta ttagtttgtg gtactgtcct
     1801 tgattgcagg atgcaaaaag ggccagaaac atgaaacagc atgttataat ttttcgtgaa
     1861 gccattgtaa atcagtaaag gtattttctt cagttttccg gtttcaggtt tttcagaacg
     1921 ggtctggcct gcaaaaacgc gtccggtttt aatcagtgta agttagtaaa ttattatcag
     1981 tggcattcat taatacttgc ccgctttaat atgattccgg acttaaaata atttaataca
     2041 tttattcaat aggacagtat tgaaagttgt cttgaatcct ctctgatttt actatattag
     2101 tcccaacaat attaaactga tctaaaaaat acatgtaaag caaaccctgt cacgatgtat
     2161 cccggcggct gatacggccg tgtgctttat tccgccgtga aaagactccg gagatgccgg
     2221 aaaatgtctt taacaccggt gtgattttcg gacatttgac agctttgtcc cggcatgatt
     2281 tgcaaccaga aataaacagc agcactatga gaacttcagt aatgtttttt ttgattattt
     2341 acatatttgg cagctgtacg ccgtccaaac atgattccgg cggaccggaa tgggcagttt
     2401 cagtcattcc ttcatcggta aggctcgatc cggtcagcaa cgaaattatc gataaccggt
     2461 ttgtccgcct gaaagggaat aaaaatctca atcgggatgt tctgaagaaa aattcaatat
     2521 acgatggcaa ggctgtgtca ctgcatgctg ccaggggaga gtacatttca tttcaactgg
     2581 tattaaccag tttgacaaag gagacgcttg acaacatcag agtaacgatg gaacccttca
     2641 ggaagggaga aaaagaattt aaagccggac ctgaactttt tctcgaatgg gcagttgagg
     2701 ttaaaacacc cagcaccggc tatcccgaag cttcactggg caacggctgg tatccggatg
     2761 cactcatccc gttttcctat atccaggacg attcatccgg agtaaagtgg cggtggacat
     2821 acccgctctg gcttcctgac ttcaacaacc ggattgataa ccagagatcg ctgctcgtct
     2881 ggatcgatca gtatatacct tttcccaggg atgaagccgg ccctggtatc tattcgtcgg
     2941 aactggcggt aacaatcggg ggattcacca ggaagattcc tgtcaatctc actgtatggg
     3001 atttcgcact gcccaacgag aataaattca aggcgggtct tcagcatgaa ggtttcatga
     3061 aaaacatgac cgaaaggatg gaacttgaaa tataccagct ccttaaacgc aacaggattt
     3121 caatgcttga tcccacctac gaacccggac tgaagatcgg aagtaacggt aatattgcca
     3181 ttgactggaa agctcttgac gaacgccttg gcaaatattt cacgggaaag gcgtttactg
     3241 ctgaatacgg ttacagttac ggtccaggtt acggtgagcc gatagagact tttgtgctgc
     3301 catttgatgt ttccgggaag cacggtacga aaggctggcc cgatatcggc gggaccgatg
     3361 ttgaacgtaa tccgtcaaac agggcaatct ataccgaatg tatcagaaag gtgcgcgatc
     3421 attacaaaac aataattgac ccggaaaaaa ctgatatcac tgtatatctt aacggactgg
     3481 atgaatccta ttttcctgaa gcgtggagca gaatggtcta ttacggcaat attttccgtg
     3541 aatattatcc cgaagccggt ttccgtatcg atggcggata cagtgaggaa gcgctggaga
     3601 tcgtaaagga ctccatcact tcatgggcat cccacaccat tgaatatgat tttgaaacgg
     3661 taaaaaaata ccaggatatg ggcataaaga tatggcttta cggtccgctg ctctatgaga
     3721 gcaagataaa cagctgggtc ggcagttcga ccttcctcga ccttcccctt gtaaacgaca
     3781 gggctataag ctggagcagc tggaaatacc ggacatattc atggctgagc tggggggccg
     3841 gggcaggctg ggtcaacgga tggtacgatc cggagtcatg gaaggacgca tcaaaagccc
     3901 ggccggaaac tgaggctgaa ttcacttata aaaagatcaa tgggaatgca cttttgattt
     3961 atgctcccgg cgttgttccc aatgtcagcg gaccgtgtcc ctctgtcaga ctgaaggcca
     4021 tgcgtgacgg ggtgcaggaa tatgagtacc tgagactgct gtcagaagcc gatggaaata
     4081 ctgcaagggc agattctatt gtaaatgaaa taatcaggga accttttggt ccgaaatcca
     4141 tcgggaatct cgacgtgtgg agtttcgatg ccggcaaatg ggatgcacaa aggattgcca
     4201 tgggcaggat gattgaaaaa aagctgcaga tcaggaataa ataggttcaa ataaatgtga
     4261 atggcaataa gtttatcagc aatgttcagc taaaactgaa tatgaaggta tccggtaagg
     4321 accggtgaaa gaaagatatc atcaggattg ctatcctgaa ttatttcatt gtcagttaat
     4381 aatcagtgct gatttacaag ttcctcgatg cgttttgcca ttgctttacg gcttaaatac
     4441 aattcggcca gatttctgct gaatgtactt acatcaataa ccatattcct tacaatctca
     4501 ttcgcgaagg caggatcgcc tttctccttc agcatccata tgtactcgta gtcctcaata
     4561 ccttccctga gaagctcaaa tctgattgag gacacagggc cgttaacatc cggctgaccg
     4621 gtatatcttc ttgtgtgatt gcccggataa atgagtgatc cctccccgtt cagcacataa
     4681 cccccgctac attcaaagcc tgaaataaat gtgaccgggt caagccatgg atcaggggtc
     4741 tggttccaga aatttgccgc ccagtaaagg aacccgttca ttttgtagcg ttctgtaatc
     4801 cacgggacca taagcggatc aagggcagga gcatcaatga aatagtttgg ctgaggatat
     4861 gcctgatcac atgagatata ccatgtggcc tcgcctcctt ttgcctgttc ctcaagtaat
     4921 gcctgcttaa cttcaggatc gttcagggca tttccatgaa cacagaaatt gttgacataa
     4981 cctcgcagtg ttccccaatc cggatcatca ctgaccggag attccgtagc gagaaagggt
     5041 acgccgggga cagcttcgtg gacaagctgt gcatagagcc gggtttcctc atactctttc
     5101 ttagtgtttg gttcatcaat cggactgttg aagacaaggc ggccgagcca gccccgttcc
     5161 ctgaaaaagg actcaacgga tgtgagatag ctttttacaa ctctgttgaa aaccggggag
     5221 aagggatccg ctttcagctg cctgctgatt tcatggggga gggtttccag gatcactctt
     5281 ttcaacttaa gcttattcat gtaataatca tatctttcct cattgaattt cagatggacc
     5341 ttttctccct tcaggataat ttcagggtaa agaggatcat tgaaccaggt ttccattctg
     5401 tgatcaaaca ggaaatcgaa ataattccgt aaaagctctt cagaggcggg ttctgaacca
     5461 tgtaagttgt agaatctggt tatttcattt tttgaaatat tcatataggt aataatgtga
     5521 gtctcatcag gcagcgagaa tccatacact tccaggctga caccgattgt ttcaattacc
     5581 cttccgaatc tctcaactgt tattgtcccc ctgtagattc ctttgggagt accggaggat
     5641 atgaaaacat ctatccacac cggcctgttc cccacaacta tatactgggc ctgcatactg
     5701 aatggttcac tcaggggtgc aagggcatcg ggccagtaac ctgttgcccc gaccggacca
     5761 gaagccgcat aaatctgaat ataatgttca aggaatagtt ttacgttctt ctgatctata
     5821 acatcacccc gggctgactt aaggcctgag gcttttacga agaatccgcc gggtgcctcg
     5881 ggcctccagc ccggagggac cggagctgaa atcacgacct ggaaaggaac atgttcgtta
     5941 cctgctccgg ctatggaaat ctgcttgtct gctgcagacc atacaagatt tgcggcctca
     6001 ggtctgtcat ccggcctgac tttctgttct cccgggacag cccatacctt tatcccgtca
     6061 ttgttgccgg gtgattgagc aagcagggcc gtaccggatg ataaaactgc aaataacaca
     6121 aagcacaggc aaaatgggta aaagcccggg aaaatagcgg ccggatcacc tgatccggcc
     6181 gctgactgaa agttttcagg aatgagaatc atcagggaga tgtgtatccg ggattttgtg
     6241 tcagggcttt attaagccgc agatcctttt gcggtattgg caacagataa tcttcaactg
     6301 aatagttctg ttgaaaatca ggatcggtaa ctttatcaag gatccgcttt cgctgaatgt
     6361 cgaaccatct gtcatattcg aagcacagtt cccagttcct ctgctgtatt attgcttcat
     6421 caaactcttc ctttgtcatg gacgttgtaa agagcgggtc gtcaggattc gggacgccac
     6481 cggttgccct gttcactatc tggttaaccg catcgcaggc agcctgtgta ggtccgttta
     6541 cctgattctc ggcttcggca aacagcagga gggcatcagc atatcgcagg ataggcatgc
     6601 tctgatatga aacgttcttt tccatgtttt cgggagtgtc atagagatat ttccttacat
     6661 agggagtgct gccgtcccat tccaggtatg ttgttccggt gccgaaccat gggtcctcaa
     6721 gaagcagata tgcatgtttc ctgggttcct cgggaaaagc ttcaccccat cttttttcag
     6781 tcctggtgtc accccatcca cccgccaaat cgccgggcag ccagatctgt ggttctatga
     6841 catattcttc ttccgaatta tggaaacacc acatgaattc aggtccgtag gtgttttcca
     6901 tcttaaaaac atcctcaacg tccggaacaa gccagtaatt gccagcatct attacctgtt
     6961 tggccatatc ccttgcctta acatagttgg aaggttcatt gagagggaaa cctgccattg
     7021 ttatatatac cttggccaga agggtcttgg cagcatctgc tgccggacgg gctctgtttt
     7081 cttctcccca tttgaccgga agatgctgtg tcgcaaaaag aagatcattt tcaatcagtt
     7141 cgtagacctc tgccacggat gccctgggta tatcaaccct ggtggcatcg gtattctcgt
     7201 caataagggg tacgcctcca taatagcgta caagcataaa gaagttccat cccctcagga
     7261 attttgcctg agccatcagc tcatcagcct tttcctgcga tattccggtt acctggcctt
     7321 cattgagggc ttttattgca ggattgataa aggctattgc ccggaagtgc ttttgccagc
     7381 atccacttcc aaaatcgtca ccgataacga gatcgccgcc ccagacctgg tcatccattt
     7441 caaagatttc cggatatccg taccagtcgt agaatgtcca tcccccgaac aggacgtcca
     7501 ttgaggcagt gaatgctgat tcaacctgcg agggtgttgt gaaaaagttg tcaggtccca
     7561 cgaaatcggt gggtttctca atcagcagtt gttcacagcc tgaagcaaag acgagaatgg
     7621 ctgctgacaa aattgatatt gttaatatgc ttgaaaattt ctttttcatt ttcctggtga
     7681 taattagatt taaaaagtaa aatcgatacc gaaagtgaac atcctggcag gcggataaac
     7741 gctctgatcg acgccaatca gtgcatcatt ataggcgtat tgtgctacct cagggtcata
     7801 accggtgtat ctggtcagag tcagcagatt tgttccggta aaatacagcc tggctttcga
     7861 aaacttgatt tttcttaaaa gattggagtc gaaattataa gccagggtaa gtgttttcag
     7921 tcttataaag gaggcatctt caacccatcg tgatgtggct cctgatcctc cgccgaagat
     7981 gtatttgttc acaagatgct gatccagcac ccactgaccg tcatagaggg caggcacatc
     8041 agtatcctga ttttcgggtg tccagtaatt ccatactttc ggatcggttc cttcccagtt
     8101 tgactcccgg cggatccgta aagtgttgaa caactggttt ccatatgagc cgtttatcag
     8161 gaaggaaagt tccaggccct tgtaagtgaa catattggtc catccaacag tgaaatccgg
     8221 atatccatag cctatggtgg ttctgtcgaa gctgtcaacc tggccgtcag gaatcccgtc
     8281 aggaccgctg atatccgcgt atttcggcgt tcccggaagc tggccgtagc ttcttgcttc
     8341 tgcctcttcc tccagcctcc atagtccgag atatttccag cctctcatct gaccaaaagg
     8401 ctgcccgact tcgaggaaca tgtactctgt acccaggctg tatcctccgg atgtgggtgt
     8461 atatcctatt cttgtcactc ccggtccgag atcaagaacc ttgttccggt tcagtgtaaa
     8521 attcacactg gtattccatc tgaatcgccc ggaaacagga tcaccgctaa tcagtatttc
     8581 gatacccttg ttttcaatcg atccgatatt gtcaataacg cttgacacac caacataatc
     8641 gggcagctgc cttggcatca gcagatcctt ggttactttc ctgtaaacat ctattgtgga
     8701 ggtaagacgt ccgccgaaca tagaaagatc gattccgata tttgcctggc ctgtggattc
     8761 ccacttcaga ctgggattgg ctatgtctga aagcccgaat ccgatattaa gattatctcc
     8821 gttgttgtag gggtatctga agcctgagcc aagtctggcc agggatccaa aaggacttat
     8881 agcctgatta cccgtgacac cgtaactcac cctgagtttg agatcggaaa ttatttccga
     8941 gtcccgcaga aactcttcct gggaaattct ccaggcaacg gagcctgacg ggaaataacc
     9001 ccacttatta ttcagtccga acactgatga accgtcagcc ctgtaactca gggttaagag
     9061 gtatttatcc agcagaccgt aattgatgcg tcccataaat gagataagtg ttctcttcga
     9121 tgcccatgaa tcgacagtca ctgatttggc gccatccata ttatcggttc ccagctggtc
     9181 aaccaggaag ttctgtcctc tcatgctgct gcccgatgag cggctgaaaa tttcttcagt
     9241 cacccctgtg atggtaatgt ggtgaattcc gaaagtctgg tcgtatgtga gaatgtttgt
     9301 attctggtaa gaatttctga aatcgtcatt gattcttccc acaccattca tggcaaaccc
     9361 gtttcgtgtc ttggtatttt catacttaag atcgtatgat gacgtaaaag tacctccccc
     9421 catgacttta agggtaagac ccttcaacag ttgaaaatta agaaaaacat taagattgtt
     9481 ctggtaggtc ggattgtcta ttttagtttc cagtgctgtt gctacaggat tccatgtatc
     9541 ataagcacca tatcccgagc ggtgcttcca gtaattgcca ttttcatcat aaaccggttc
     9601 agttggagcc catcttggtg catcattcac agatgtgaaa agattggctt caaacggggg
     9661 tgatttatat ttttcgaagg tgtatgagta attcaggcca aaatcaaccc agtcagtaat
     9721 gtcagctgca agatttgttc tcagcgacag tctgttatag gctgaattca gaaggatgcc
     9781 cttgtgatca aggtagcctg tggaaaccat ataactgagt cgttctgttg ccccgctgat
     9841 tgcaagattg cttgtctgca ttatgccggt tttataaatc tcatcctgcc agtccgttga
     9901 gtgggtttta taatattcaa tgtcgtcatc cgtgaaatac agctttggag tctgtccgta
     9961 agcagtattg aacttgttct gaaaattatt atatcttgca aaatcatagg cgttcattac
    10021 gggcagcttt ctcgcaaggt tctgaatacc cacgctgtaa cctccgtcaa ttacaggttt
    10081 gccggtttta cccagctttg ttgttatgag gataacacca tttgatcctc tggaaccgta
    10141 tatagctgta gaagatgcat cctttaagac t
//
LOCUS       DAOSPX010000082        10117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6453_length_10117_cov_4.163089, whole genome shotgun sequence.
ACCESSION   DAOSPX010000082 DAOSPX010000000
VERSION     DAOSPX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6453_length_10117_cov_4.163089"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLZ75_05700"
     CDS             <1..1107
                     /locus_tag="PLZ75_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71927.1"
                     /translation="ADIFRNKTIAEILYFLKILPVYRIRDGFSSLKENDEIFKKTVDV
                     LRNKNGLVILPEGDHAGYRRLRQLKKGICRIAFQADEASGFEMKIKIIPVGLEFTNYQ
                     RFRQVLTVVYGKAVEVDEYHELYRKNPEVAHNQLRERLSGEMKMLMVHIDSKEDYEAI
                     DELRSLVNGPYSDDVRFPKLFRDRMLIDKLNNLKITNTELYRKICSLSLNVKQKAGEL
                     KIDYPLLRKNRHPLGWLIAGIFGLIASLPVFIYGTIFTLVFLGIPNSQIPKIRDKQFH
                     SSIKYGISVGLALVFIPVFLIAFLLIFSPFWLGLILFLALPVSGLFAVNYIHYLKRIT
                     GGFRIRKYLRRNDSEYMKLKSDHDELIKQIGKLA"
     gene            1107..1913
                     /locus_tag="PLZ75_05705"
     CDS             1107..1913
                     /locus_tag="PLZ75_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS71928.1"
                     /translation="MGNSEIFSGKTAWITGASSGIGKGLVHAFLERGASLVISSNDAE
                     GLEKVKSEYAGISQDILSAPFDLSDTDGIESLVENVVKKTGRIDYLLNIGGVSQRASV
                     ADTPLWLDRKIFEINYFGTIALTKAVLPYMIRQGSGHIAATSSISGRFGFPLRSAYSA
                     SKQALHGFFETLFLENKKNNIRTSVIIPGRVRTSISLHALDHEGREHGRMDEGQAKGI
                     SPEKAARIIIRGILRNKREITVGGSELLLLYVRRYFPRLFFRIGDKISAT"
     gene            1926..3005
                     /locus_tag="PLZ75_05710"
     CDS             1926..3005
                     /locus_tag="PLZ75_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOS71929.1"
                     /translation="MNDYSICGIQQAGIGVRNLKESWNWYIGTFGMDCKIFEEEAEAK
                     IMLPYTGNQARKRHAVLAVNLQGGGGFEVWQYKEREPVPLKGEISLIDIGILACKMKV
                     KDIGKAYSFFRNTGAVILNEPTADPSGRKTFYMKDPSGNLFHINEGDGWFMDTGHISG
                     GTCGAVIGVTDIEKARGLYTDVLGYDQVIYDITGTFPDLANIPGDNEPVRRVLLANSK
                     SFTGPFSKIFGQSMMELICSIGRKGKKIYEGRYWGDPGFIHICFDIKGFDHLKIRCSE
                     KGFPFTVNSKESFKGQGFDMGEAGGHFAYVEDPDGTLIEFVETLKLPLVKKLGWYLDL
                     SKRKAEPLPGWMLKMLKFSRVKAVK"
     gene            complement(3069..4268)
                     /locus_tag="PLZ75_05715"
     CDS             complement(3069..4268)
                     /locus_tag="PLZ75_05715"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOS71930.1"
                     /translation="MKKLSLVFLIPAILISCSNDRNLARRADRLHKCFLTVDTHCDTP
                     ILLVSSDYDLGERHEEACVDFPRMKEGGLDAEFFAVYLAQGPRNGEAYNIVHQKTLEI
                     FDSIHSNLRKNSRLAELALTPDDACRLKKENRISVFIGVENGYAIGMETDRIREYYDL
                     GARYMTLCHSSNNDICDSSTDPRGPEHNGLSEFGREVVKEMNRTGMMVDVSHISDEAF
                     YDVIRVSKAPVIASHSSCRALCSSPRNLTDDMLHSLKSNGGVVQICILSSYIKTPEPN
                     PEMDARFEELRSKYGEFGKLGEDQKKSYRKEMREIFDKYRKLATVADVLDHIDHVVQV
                     IGVDHVGIGTDFDGGGGVEGCITVDQMKNITIEMLRRGYSNKDIKKIWGGNIMRVLRE
                     VQESASV"
     gene            4606..6246
                     /gene="lnt"
                     /locus_tag="PLZ75_05720"
     CDS             4606..6246
                     /gene="lnt"
                     /locus_tag="PLZ75_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOS71931.1"
                     /translation="MRRYSLIALSAAGGMLSGLAWSEFCPGLILLVSFVPWLLIEDHL
                     YLNPGKYNLNSCFILLLPGFVLFNTIVLSWIRIASIPAAIAVITEMSFLMTLVFCLAH
                     SVRKKAGRTAGYAALTIFWLAFENLSLHSSFLTPWINLGNGLAKNILFIQWYDITGVA
                     GGSLWILISNIFLTLFLLNRSRKKRAALTYASLWLMITAVPAAFSVSEYSRPEESKEG
                     QGSEIVIVQPNFDPYTEKFSIPFEQQLDTALKIAEGMLTDSSRWLIFPETMIDDPVNE
                     ADAGNNKYILTLRDFAAGYNDLNIIAGLVSYRIYPGSEKAPTRSARLTNLSEHYADHY
                     NSAFRIDAINYPSIYHKSRLVPGIEMHYSPFLEKVTEWILPDMGGTMWGYGTQDSRTC
                     FIHSATGHRIAPVICYESVFGDFVAGSVREGAEALAVITNDGWWKNTKGYYQHLGYSS
                     LRAIETGRPVVRCANTGISCITDKRGKRLQETEWWTAGSLKGIIVPETEITFYVRAGD
                     YIFNAASVACPVILIYIFGPEMIRRMRRAFPPKKPDRN"
     gene            complement(6322..>7055)
                     /locus_tag="PLZ75_05725"
     CDS             complement(6322..>7055)
                     /locus_tag="PLZ75_05725"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS71932.1"
                     /translation="LLGKIRFAHDSEVTHTGVFRFFLVVIVFTFVVYLTTGLFGNPLK
                     GIASLLPSQETSWFSQAERATLPQQQEPAFATFSPLAKNLQCQEPKYGDRFRLPYGLR
                     GYFDYRQGLECAKELGKPALLDFKGHACANCRMMEARVWSDPEVQRRLREDFVIIALY
                     LDDRTQLPENEWIVSQVDGKTKKTIGKVNEDLEISRFRTNAMPLYVITDHKGNPLNAP
                     MTTNLNVDEYRKWLDEGLQLFKQKI"
     assembly_gap    7056..7065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7066..8280)
                     /locus_tag="PLZ75_05730"
     CDS             complement(<7066..8280)
                     /locus_tag="PLZ75_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOS71933.1"
                     /translation="MNKTRFLFLIFMLITISARSQIFDPAVWNFGYEDKGNNVYEIVI
                     SATIEEGSHIYSMDIPEGGPIPTLIRFDSSSLWTPEGDSYEAISPEEIFDEAFGMKIK
                     SFSSKAEFRQKISGNAPAFTVSGAVNYMACNNVTCSPPKDVEFEINIRRGSSAAEGGG
                     MQPAEGKTTGRGLAGFLLISMLAGFAGILTPCVFPMIPMTVAFFSRGSETRGRSAMNA
                     IIFGLSIILIYTALGLIVSLSSAGAGFANTLSTHWIPNILFFSLFVIFAISFLGAFEI
                     TLPNTWVTGADSRVDRGGVLASFFMGLTTVIVSFSCTGPIVGALLVEAATGDVLRPAI
                     GMFGFGLAFAMPFTLFALFPSVLKKMPKSGGWLNSVKVVLGFIMLAFSFKFLSVVDSV
                     YNIGLISRDVFLI"
     gene            complement(8368..9927)
                     /locus_tag="PLZ75_05735"
     CDS             complement(8368..9927)
                     /locus_tag="PLZ75_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOS71934.1"
                     /translation="MGNKDLIIDASDSEVSIALLENRQLTELDREKRTVRFSVGDIYL
                     GKVKKIMPGLNAAFINVGYERDAFLHYLDLGPQFRTQNKYYIAALQRQGRPGPVHRFK
                     PDPDIDKNGKIKDVLTVGQTVIVQITKEPISTKGPRLSSEISIAGRNLVLIPFSDKVS
                     ISAKIENPEEKNRLKSLLQSIKPKNYGIIVRTAAEGKKVAVLDAELRELVKKWESTFS
                     SVKKDIKAPKLFIGEMNRTTTILRDLLNVAFSSIHVNDSVIAGEIRTYINEIAPEKAK
                     IVKIYQGRVPIFDHFGVNRQIKALFGRTVSFRHGAYLIIEHTEALHVIDVNSGNRSRT
                     GNDQESNALEVNLEAAAEIARQLRLRDLGGIIVIDFIDMQSGENKQILYEKVKELMQT
                     DRTKHNILPLTKFGLMQITRQRVRPEMHIVTNEKCPACSGTGEIKPSILFTDELEKNL
                     EFIVDKIKTRIIYLNVHPFIAAYLKKGLFPIYLKWSLKYRILLRVQAVVSAQYLEYHY
                     LDRHNNEIDLI"
BASE COUNT         2778 a         2426 c         2271 g         2632 t
ORIGIN      
        1 gccgatatct tcagaaataa aaccattgca gaaatcctct atttcctgaa aattctgccg
       61 gtttacagga taagggacgg cttcagcagc ctgaaggaaa atgatgaaat atttaaaaaa
      121 acagtagatg ttctgcgaaa caaaaacgga ctggtaatcc ttcccgaagg ggaccacgcc
      181 ggctaccgca ggctccgcca gctgaagaaa ggcatttgca gaatagcttt ccaggcagat
      241 gaggcttccg gttttgagat gaaaatcaag attatacccg tcggactgga atttacaaac
      301 taccagagat tccggcaggt actcacggta gtgtacggca aagcggtgga agttgatgaa
      361 tatcatgaac tttacaggaa aaatcccgaa gtggcccata accagctcag ggaacggctt
      421 tccggagaaa tgaaaatgct gatggtccat atagacagta aggaagacta tgaggcaata
      481 gatgaactga gaagcctggt aaacggacct tacagcgatg atgtcagatt tcccaaactg
      541 ttcagggaca ggatgctgat cgacaagctc aataacctga aaatcacaaa taccgaactc
      601 tacaggaaaa tatgctcact gagccttaat gtcaaacaaa aagccgggga gctgaagatt
      661 gattatcccc tcctcagaaa aaacaggcac ccgcttggct ggctcatagc cggtatcttc
      721 ggacttattg ccagtctgcc ggtatttatc tacggaacca tattcaccct ggtcttcctg
      781 ggaattccaa attcacaaat tccgaaaata cgcgataaac agtttcattc ctcaataaaa
      841 tacggcatat cggtcggact tgcactcgtg ttcattcctg ttttcctgat agcctttctg
      901 ctgatattct cgcccttctg gctgggactg atcctcttcc ttgcactgcc tgtttccggc
      961 ctttttgcag tgaattacat tcattacctg aaaagaataa caggcggatt ccggataagg
     1021 aaatacctga ggagaaatga ttctgaatac atgaaactga aatcagatca tgatgaattg
     1081 attaaacaga ttggaaaact tgcctgatgg gaaactctga aattttttcc ggcaaaacag
     1141 catggatcac aggcgcatca tccggtatcg gaaaaggcct tgttcatgcc ttcctcgaaa
     1201 ggggagcatc actggtaatt tcctcaaatg atgctgaggg acttgaaaaa gtaaaatctg
     1261 aatacgcggg aatatcacag gatattttgt ctgccccctt cgatctgtcc gatactgacg
     1321 gtattgaatc cctggttgag aatgttgtga aaaaaaccgg aagaatcgat tatcttctga
     1381 atataggcgg agtgagccag agggcatcag ttgcagatac tcccctgtgg ctcgacagaa
     1441 aaatatttga aatcaattat ttcggcacaa ttgcacttac aaaggcggtt cttccctaca
     1501 tgatcagaca gggatcaggc catattgccg caacaagcag catatccgga agattcggat
     1561 tccccctgcg ttcagcttat tccgcatcca aacaggccct gcacggtttc tttgaaaccc
     1621 tattcctcga aaacaagaaa aacaatatca ggacatccgt gataataccc gggagggtga
     1681 ggacgtccat atccctccat gctcttgacc atgaaggaag ggaacacgga agaatggacg
     1741 agggacaggc aaagggtatc agtcctgaaa aggccgcaag gataattata aggggaatac
     1801 tcaggaacaa aagagaaata acagtgggcg gaagcgagct cctcttgctg tacgtaagaa
     1861 gatactttcc ccggctcttt ttcaggatag gtgataaaat aagtgcaaca taaaacaata
     1921 agtaaatgaa tgattattca atctgcggga tacagcaggc aggcatagga gtcaggaacc
     1981 tgaaggaatc ctggaactgg tatatcggta cgtttggaat ggactgcaaa attttcgagg
     2041 aagaagctga agctaaaatc atgctgccct ataccggcaa ccaggcaaga aagcggcatg
     2101 cggtgctggc tgtgaacctt caggggggcg gcggttttga agtatggcag tacaaagaaa
     2161 gagaacctgt cccgctgaag ggagaaatca gtctcattga cataggtata ctggcctgca
     2221 agatgaaagt aaaggatatc ggaaaggcat attcattctt caggaacacg ggcgccgtga
     2281 tccttaatga accaactgct gatccttccg gcaggaaaac tttctatatg aaggaccctt
     2341 ccgggaatct gttccatatc aatgaaggtg acggctggtt catggataca ggccatatat
     2401 ccggaggaac ctgcggggcg gtaatcggcg ttacggatat tgaaaaggcc agaggccttt
     2461 atacggacgt tcttggttat gaccaggtta tctatgacat aaccggtacc tttcccgatc
     2521 tggccaacat tccgggtgac aatgaacctg taagaagagt gcttctggcg aactcaaaat
     2581 cattcaccgg acctttcagt aagattttcg gacagagcat gatggaactc atctgcagta
     2641 ttggcagaaa gggaaagaaa atttatgagg gaagatactg gggggatccc ggctttattc
     2701 atatatgctt cgatatcaaa ggatttgacc atctgaaaat cagatgcagc gaaaagggat
     2761 ttcccttcac cgtcaacagc aaggaaagct tcaagggaca gggtttcgac atgggtgaag
     2821 ccggcggaca tttcgcctat gtggaggatc ccgacggcac tctgattgag ttcgtggaaa
     2881 cccttaagct gccgcttgtc aaaaagctgg gatggtatct tgacctttca aagcgcaaag
     2941 ctgaaccact gcccggctgg atgctgaaaa tgctgaaatt ctcccgggta aaggccgtaa
     3001 aataatagtc tgccggttaa taggattttg ccgggagccg gatcgcaata aacaaaacaa
     3061 tgccgtggtt aaactgatgc tgattcctga acttcacgca gcacccgcat gatattcccg
     3121 ccccagatct tctttatgtc cttgttcgaa taaccgcggc gcagcatttc gatggtgatg
     3181 ttcttcatct gatcaactgt tatgcagcct tcaacaccgc ctccgccgtc aaaatcggta
     3241 cctatgccca catgatccac cccgataacc tggactacat ggtcaatatg gtccagtaca
     3301 tcagcaacag tagcaagttt cctgtacttg tcgaaaatct ccctcatctc cttcctgtat
     3361 gattttttct gatcttcgcc gagcttcccg aactctccgt atttcgatct cagttcctcg
     3421 aatctggcat ccatctccgg attcggttca ggagtcttta tatagctgct gagaatgcag
     3481 atctgtacca ctcccccgtt tgacttaagt gaatgcagca tatcgtcggt aaggttccgc
     3541 gggcttgagc acaatgccct gcaggaagaa tgggaagcaa tgacgggagc ctttgacact
     3601 ctgattacat cgtaaaaagc ctcatcggaa atatgcgaga catccaccat catgcctgtc
     3661 ctgttcattt ccttcaccac ctccctgcca aactccgaaa gcccgttatg ttcaggtccc
     3721 cgcggatcgg ttgaggaatc acatatatca ttgttgcttg aatgacaaag agtcatgtac
     3781 cgggcaccaa gatcataata ctcccttatc ctgtctgttt ccatgccaat ggcatagccg
     3841 ttctcaacac ctatgaatac cgagatcctg ttttcctttt tcagccggca tgcatcatcg
     3901 ggtgtcagtg ccagttccgc cagccgggaa ttctttctca gattactgtg tattgaatca
     3961 aaaatctcaa gtgttttctg atgtacaata ttgtaggcct ccccgttccg ggggccctgg
     4021 gccaggtaaa ccgcaaagaa ttcagcatca agaccacctt ccttcattct tggaaaatcg
     4081 acacaggctt cctcatgcct ttcgccaaga tcataatcac tgcttacaag aagtatcggc
     4141 gtgtcacaat gtgtgtcaac agtaagaaag cacttatgca gtctgtctgc cctgcgtgca
     4201 agattcctgt cgttgctgca tgaaataaga attgcaggta tcagaaatac cagtgataat
     4261 ttcttcataa atatggtaac tatattaaat gcaagataat aattcccctc aggcctgatt
     4321 tcttaaacaa agttatttaa caaatattaa cattgaaaaa agtcccgtca gcctgcaggt
     4381 tgacgggaca gccatgaaaa gaatgaaaaa ttgcttttca gataataaaa agcttccttt
     4441 aaagttttga cactttatat cttacaaaat tttcagcatg cactttcaag atgcaaacat
     4501 atatcaatcc gctttataaa acaatatctg agcacttttt taaacagaag ccgtgaaaac
     4561 gggatgtttt ttgtaagttc accctcttta ccgccaccgg ggaaaatgag gaggtattca
     4621 cttattgcac tgtctgctgc cggaggaatg ctttccggac tggcctggtc ggaattctgt
     4681 ccgggactga tactgctggt atctttcgtg ccctggcttt tgattgagga tcacctttac
     4741 ctcaacccgg ggaaatacaa tctgaattca tgtttcatcc ttctccttcc cggttttgtc
     4801 ctgttcaaca caattgtcct ttcctggata agaattgcca gcattcctgc cgcaattgcg
     4861 gtaataacgg agatgtcatt ccttatgacc cttgtattct gtctggcgca ttccgtgaga
     4921 aaaaaggcag gaagaacagc cggatatgcc gcacttacaa tattctggct ggcttttgag
     4981 aatctgtccc ttcattcgtc ttttcttact ccctggataa acctgggaaa cggccttgcc
     5041 aaaaatattc tgttcatcca atggtatgat ataacaggag ttgccggagg aagcctctgg
     5101 atactgattt caaacatctt tctcacactc ttcctgctta accgctcccg gaaaaagcgg
     5161 gcagcactga catatgcctc gctctggctg atgatcacag ccgttcccgc ggcattctcg
     5221 gtatcggaat acagcagacc ggaagaatca aaggaaggcc agggaagcga gattgtcatc
     5281 gttcagccca attttgaccc ttatactgaa aaattcagta ttccctttga acaacagctt
     5341 gatactgccc tgaaaattgc tgaaggcatg ctgacagaca gtagccgctg gctcattttt
     5401 ccggagacaa tgattgatga tccggtaaat gaggctgatg ccggcaacaa caaatatatc
     5461 ctcaccctga gggattttgc agccggatat aatgatctca atatcattgc aggtcttgtc
     5521 agctacagga tataccccgg ttccgaaaaa gccccgacac gatcggcaag gctgacaaat
     5581 ctgtcggaac actacgcaga ccattacaat tccgcattca gaatcgacgc catcaactat
     5641 ccctccatct atcacaagtc caggcttgtt ccggggattg agatgcatta ttcccccttc
     5701 ctggaaaaag taaccgaatg gatactgccg gatatgggag gaaccatgtg gggatacgga
     5761 acgcaggaca gcagaacctg ttttattcat tccgcgacag gccacagaat agccccggta
     5821 atctgttatg aatcggtatt cggtgatttt gttgccgggt ctgtaaggga aggggcagaa
     5881 gccttggcag taataacgaa tgatggctgg tggaagaaca caaaaggtta ttaccagcat
     5941 ctcggatatt catccctcag ggccattgaa acaggaaggc ccgtggtccg ctgtgccaat
     6001 acaggcattt cgtgcatcac ggataaacgc gggaaaaggc tgcaggaaac agagtggtgg
     6061 actgcaggca gtctgaaagg tataatagtt ccggaaacag aaatcacctt ctacgtcagg
     6121 gccggtgatt acatattcaa tgccgcatct gttgcctgcc ctgttatact gatctatatt
     6181 ttcggcccag aaatgattcg gaggatgcgc agggcttttc ctccgaaaaa gccggacagg
     6241 aactgaccgc cggcctggct ccggaacacc gggagtatga caggaaaata ttaagacttt
     6301 atgaaagggc agcccggatt atcagatttt ctgtttaaac agctgcagtc cctcatccag
     6361 ccacttcctg tattcatcaa cattcagatt cgtggtcatc ggagcgttta gcggattgcc
     6421 cttgtgatcc gttatcacat aaaggggcat tgcatttgtc ctgaacctgg agatttcaag
     6481 atcttcattc acctttccga ttgttttctt tgtcttaccg tccacctgcg acactatcca
     6541 ctcgttttcc ggaagctgcg tcctgtcatc aagatagagg gctatgatca caaaatcttc
     6601 tctcagccgc ctctgcacct cagggtcgga ccataccctt gcttccatca tcctgcagtt
     6661 ggcacatgcg tgccccttga agtccagaag agcaggcttc ccaagttctt tcgcacattc
     6721 aagaccctgc ctgtaatcga aatagcccct gagcccgtag ggcagcctga acctgtcccc
     6781 gtattttggt tcctgacatt gcaggttttt cgcaagcgga ctgaatgttg cgaatgccgg
     6841 ctcctgctgc tgaggcagtg ttgccctttc ggcctgactg aaccaggatg tctcctgtga
     6901 gggaagaagg gaagcaatcc ccttcagggg attaccgaaa aggcctgtag tgaggtatac
     6961 cacaaaggta aaaactatga ccaccagaaa gaatctgaaa acccccgtgt gagtcacttc
     7021 actgtcgtgg gcgaagcgaa ttttcccgag aaggtnnnnn nnnnntatca ggaaaacatc
     7081 cctggagatt aatccgatat tatagacact gtctaccaca gacaggaact tgaagctgaa
     7141 tgccagcata ataaagccaa ggacgacctt gacggaattc agccagcctc ctgatttcgg
     7201 catctttttc agaacggagg ggaagagggc aaaaagcgta aagggcattg caaatgccag
     7261 gccgaatccg aacatgccga tggccggcct gagaacatcg ccggtagctg cttccacaag
     7321 aagtgctcct actattggac cggtacagga aaatgataca ataaccgttg taaggcccat
     7381 gaagaatgag gcaagaacac ctcccctgtc aacccgtgaa tccgctcccg tgacccaggt
     7441 gttcggcagg gtaatctcaa aggctccgag aaaggaaatt gcgaaaatca cgaacaatga
     7501 aaagaacagg atattgggaa tccagtgcgt gctcagtgta tttgcaaaac ctgcccccgc
     7561 actgctcagg gatacaatca gtcccagggc cgtgtaaata agtattatcg acaatccgaa
     7621 aattattgcg ttcatggccg acctgcctct ggtttccgac ccccgcgaga aaaaggcaac
     7681 ggtcatggga atcatcggga aaacgcaagg tgtgagtatt ccggcaaatc ctgccagcat
     7741 tgaaataaga agaaagcccg cgagtcccct gcctgtggtt ttcccttcag caggctgcat
     7801 tccgccgcct tccgctgcgg aactgcctct cctgatgttt atttcgaatt ccacatcctt
     7861 gggcggggag catgtaacat tattgcaggc catgtaatta accgcaccgc tgaccgtaaa
     7921 agccggagca ttgccgctta tcttctgcct gaattcggcc ttgctgctga atgatttgat
     7981 tttcatgccg aatgcttcat caaatatttc ctcggggctt atcgcctcat agctgtctcc
     8041 ctccggggtc cataaggatg aactgtcgaa acggattaat gtgggtatgg gccctccctc
     8101 gggaatatcc atggaataaa tatgagagcc ctcttcaatt gttgcactga taacaatctc
     8161 ataaacattg ttgcccttat cctcatatcc gaaattccat actgcgggat caaatatctg
     8221 tgaccgggca gaaatggtga tcagcataaa aatcaaaaac agaaacctgg tcttattcat
     8281 gacttaaaat gataataatt acaaattcag taatacaata acagtgaagg gccggaaagg
     8341 ttgaatgctg ttttaagctt cccgggatca gataaggtca atctcgttat tatgcctgtc
     8401 aagataatga tattcaagat actgagcaga cacaaccgcc tgaaccctca gcaggatcct
     8461 gtacttcagg ctccacttca ggtatatggg aaacaggcct ttcttcagat atgcggcaat
     8521 aaaaggatgt acattaaggt aaatgatgcg ggttttaatc ttgtccacga tgaattcaag
     8581 gttcttttcc agttcatcgg taaaaagaat acttggtttt atttcgcctg tcccgctgca
     8641 ggcagggcat ttttcatttg taacgatatg catttcgggc cttacccgct gccgtgttat
     8701 ctgcatgagc ccgaattttg tgagaggaag gatgttatgc tttgtccggt cggtctgcat
     8761 gagttccttc accttttcat acagtatctg tttgttttct cccgactgca tatcaatgaa
     8821 atcgataaca atgatccctc ccaggtctct cagccgcagc tgccgggcta tttcagcagc
     8881 agcttccaga ttaacctcca gtgcattcga ttcctgatca tttccggtac gtgacctgtt
     8941 tccgctgttt acatcaatca cgtgaagagc ttcggtatgt tcaataatga gatatgcacc
     9001 atgcctgaac gacaccgttc tgccgaacag ggccttgatc tgcctgttaa cgccgaaatg
     9061 atcaaagatg ggaacccttc cctgataaat ctttacaatc tttgcttttt caggagcaat
     9121 ctcattgata taggtgcgta tttccccggc aattacactg tcgttgacat gaatgctgct
     9181 gaatgcaaca ttgagcagat cccggagtat agtggttgtc cggttcattt caccgataaa
     9241 gagcttcggt gcctttatat ccttcttaac ggagctgaaa gtggattccc atttctttac
     9301 gagttccctg agctcagcat ccagaaccgc aaccttcttt ccttcggcag ccgtccgcac
     9361 aatgatcccg taatttttcg gcttgatact ctgaagaagt gatttaagac ggttcttctc
     9421 ctcaggattt tcaattttag ctgatattga tactttatcg gaaaagggaa taagaacgag
     9481 gtttcttcct gctattgaaa tttccgagga aagcctcggc cccttggtgg aaataggctc
     9541 ctttgtaatc tggacaatca cagtctggcc aactgtcagt acgtccttga tcttgccgtt
     9601 cttgtcaata tccgggtcag gcttgaacct gtgaaccggc ccgggcctcc cctgcctctg
     9661 aagcgctgca atatagtatt tgttctgagt ccggaactgt ggtcccagat ccaggtaatg
     9721 aagaaatgca tctctttcat aaccaacatt tatgaaagct gcattgagtc cgggcattat
     9781 cttctttacc ttacccagat aaatatcccc gacagaaaaa cggacagtcc gcttttccct
     9841 gtccaattct gtaagctgcc tgttctccag taatgcaatg gaaacttcag aatcacttgc
     9901 atcaatgatc agatccttgt tacccactta aaaatcacat ctaatgtatc tgtcttcaca
     9961 atcaccctca cttatagata caacaaacct aaagatcgtc agacctttag gtttagtata
    10021 tcaaatcaac gaccacccgg cgatcttcta cttcttcttg tgcctgttct tcctcaaacg
    10081 ctttttacgc ttgtgagtgg ccatcttatg tcttttt
//
LOCUS       DAOSPX010000083        10040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6511_length_10040_cov_4.016825, whole genome shotgun sequence.
ACCESSION   DAOSPX010000083 DAOSPX010000000
VERSION     DAOSPX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10040
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6511_length_10040_cov_4.016825"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..665)
                     /locus_tag="PLZ75_05740"
     CDS             complement(141..665)
                     /locus_tag="PLZ75_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOS71935.1"
                     /translation="MTGTRKIYIIGFMGSGKSTMGRRLAARMGYQFLDLDDKIEELAG
                     KTIARIFSEDGEEEFRRIEAETLKGLGSDANIIISTGGGTPCHFDNMDYMRETGITVY
                     LKMTPGQLASRLRGSSAERPLLKNIPDGELVSFIGEKLSEREQWYNRAGIIVDGMNAD
                     PGLLATMIQSERGY"
     gene            complement(665..3286)
                     /locus_tag="PLZ75_05745"
     CDS             complement(665..3286)
                     /locus_tag="PLZ75_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOS71936.1"
                     /translation="MEELEFESGYPESGEETEYSPAALHSVTALPGMFRDWFLDYASY
                     VILERAVPDLFDGLKPVQRRILHSMRRMDDGRFNKVANIIGHTMQFHPHGDASIGDAL
                     VQLGQKDLLVETQGNWGNILTGDGAAAPRYIEARLSKFALEVIFNPKTTEWKQSYDGR
                     NREPVSLPAKFPLLLAMGVEGIAVGLASKILPHNFNELIDATINHLQGKEFVIYPDFP
                     TGGFIDVSKYNDGQRGGVVKVRARIEKIDKKSLVITEIPFGKTTTTLIESIIKANEKG
                     KIKIRKIDDNTSADVEIKIHLMPGVSPDKTIDALYALTDCEYSISPNTCVISEGRPSF
                     MGVSEILKHSASRTVELLKTELEIRKNELQEDWHMSSLEKIFIEEKIYRNIEECETWE
                     SVISTIDDGLKPFRNRLLRDVTQDDIIKLTEIRIKRISKYDVRKADEHIKGIEAELEE
                     VKNHLNNIIPYTINYFRQIKKKYGKGRERKTEIRNFDTIQAAKVIANNARLYINYKEG
                     FIGTSLKKDEFVCECSDIDDIIVIRKNGAYLITKVDDKVFVGNDILYAAVFLRNDERT
                     IYNIVYQDGKDGPCLAKRFAITGLTRDKEYNLTRGTPGSRIIYFSANPNGEAEVIRVH
                     HKPRARLKKLTFEFDFGELAIKGRTTMGNILTKYAVNKITLKEKGLSTLGGRKIWFDD
                     AVFRLNGDGRGICLGEFAGDDKILVITKNGYFRNTGFDLSNHFEDNILIIEKFRPGKV
                     YSAIYYDGELKYHYIKRFMIEESEKPLLFISEHPDSRLISLTEVEYPRFEISFGGKHR
                     NRENEIIEVAEFIGVKSHRAKGRRLTNYMVDNVLEIEPVVRKEGVQECGQDEEEDDQA
                     GLPAIVQDHPAQMKLEL"
     gene            complement(3324..5165)
                     /locus_tag="PLZ75_05750"
     CDS             complement(3324..5165)
                     /locus_tag="PLZ75_05750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOS71937.1"
                     /translation="MTAAGYSEEHIRTLEWREHIRLRPGMYIGKLGDGSSQDDGIYVL
                     LKEVMDNAIDEFMMGFGKKIDVSITGREVRVRDYGRGIPLGKVTDAVARMNTGAKYDS
                     KAFKKSVGLNGVGAKAVNALSSRFIIRSVRDGEVKMTEFEHGILLKDHPLEKTDEENG
                     VEVIFQPDENMFGNYFYISEYVETMLKNYTYLNTGLVINFNGNKYFSRNGLLDMLTDN
                     MSREGLYPLIHLKGDDIEIAFTHGHQYGEEYLSFVNGQNTTQGGTHLTVFREAVVKTI
                     REFYKKDYDPSDIRSSIIAAISIKVEEPVFESQTKTKLGSKDMGPKGPSIRNFINEFV
                     KKRLDDYLHKNPETSRIILKKIQDSEKERKAISSIQKLARERAKKVSLHNKKLRDCRI
                     HFNSADPRKLESTIFITEGDSASGSITKSRDVETQAVFSLRGKPLNSFGLTKKIVYEN
                     EEFNLLQAALNIEDGIENLRYNNVVIATDADVDGMHIRLLLLTFFLQFFPDLVRKGHV
                     YILQTPLFRVRNKKETKYCYTSAERSRAIETLGPNPEITRFKGLGEISPDEFRYFIGK
                     DMRLEPVRMKRNDAVNGYLEFYMGKNTPERQDFIIENLKFEEDLVEA"
     gene            complement(5479..6225)
                     /locus_tag="PLZ75_05755"
     CDS             complement(5479..6225)
                     /locus_tag="PLZ75_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOS71938.1"
                     /translation="MNILRSILVWFTGIMIMVLFFPVVFVIWLLVLPFDRNRTIVHWL
                     LIYKSLVITYLIPVWKIRIEGREKAVRKQTYVIISNHQSILDILLLNCLRYRFKWISK
                     IENMKVPVLGWYLRMAGYITVDRGNRESKEKMMEESLACLKKGISIMIFPEGTRSFDG
                     EIGFFRRGAFELAIAAQLPVLPVLLTGTRDILPKHGLVFGDYRRIMIRVLDPVPPESF
                     GTDDCDELSVKFRTRMTEVFDEMTRETGEK"
     gene            complement(6328..7275)
                     /locus_tag="PLZ75_05760"
     CDS             complement(6328..7275)
                     /locus_tag="PLZ75_05760"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOS71939.1"
                     /translation="MICIDLDTTDPCFNLAAEEYLLRNREEDFLVIGVNNPSVIIGKH
                     QVAHREADTKFVSRNFIPVIRRISGGGAVYHDRGNINFSFIIQSRSGHQVNFRKYTRP
                     VIEFLASLGTDAVLEGRNNLRVKGLKISGNAEHVYRERVLHHGTLLFNADTEMLKRSL
                     RKNTSSYLTRAVDSVPSRVANLGDLMGGRVNMEEFRSLLLDFFLRREENRLHRLTSGE
                     ESQIRLIADSKYRTWEWNYAYGPEYQFADEFEIGGESCSCRMTVKDGIIRECAISGNT
                     GLERAAEGLTGCRHMPEDMMKVFRNENIRLESPDIYKFF"
     gene            complement(7288..7845)
                     /locus_tag="PLZ75_05765"
     CDS             complement(7288..7845)
                     /locus_tag="PLZ75_05765"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOS71940.1"
                     /translation="MLENLDRQIFLFLNSGYSPFLDKLMWIISGILTWVPLYLAVLIW
                     IWLKYRRKALLILLFIILAATLTELTSVHLFKNVFQRLRPCHEPSLQGMVHIVNCHCG
                     GLYGFVSSHAANSFNVAVLSLLFIRERWFSVFMIIWALLVGYSRIYLGVHYPGDVLCG
                     SLLGALIGWSCYRLYLVAEKNIRPE"
     gene            complement(7855..8847)
                     /gene="obgE"
                     /locus_tag="PLZ75_05770"
     CDS             complement(7855..8847)
                     /gene="obgE"
                     /locus_tag="PLZ75_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOS71941.1"
                     /translation="MSSASFVDYVKIFCRSGNGGAGSSHLRREKFVPKGGPDGGDGGR
                     GGHVILRGNGQMWTLLHLKFRRHIFAEHGGAGGRQQSTGADGEDVVIEVPPGTVVKTA
                     ESGEMILEVTSHGEERILVKGGRGGLGNVHFKSPTNQTPRYAQPGEKGSEGWFILELK
                     LLADVGLVGFPNAGKSTLLSVLSSAKPKIADYPFTTLVPNLGIVACRDNKSFVMADIP
                     GIIEGAHEGKGLGVRFLRHIERNSLLLFVIPADTGDIRREYEILLNELSLYNPELLDK
                     KRVLAVSKTDLLDDELKSGLSRDLPDIPGIFVSSFTGTGLNQLKDLLWGVLNEN"
     gene            complement(8880..9458)
                     /locus_tag="PLZ75_05775"
     CDS             complement(8880..9458)
                     /locus_tag="PLZ75_05775"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOS71942.1"
                     /translation="MLNFLIFGPPGSGKGTQSVKLAEKFNLAHLSTGDMLRAEIAAET
                     ELGRKVSSIMAKGELVPDEVVIEMIAGKIDRAENVAGFLFDGFPRTVEQTVALEKMLN
                     DRKMKIDSMLVLDVEHDELVKRLVGRAELSGRPDDKDTVVIENRIAVYRGKTEPIISY
                     CRERGIYQPVNGMGSIEEIFDRLSVHVSRLAN"
     gene            complement(9470..10006)
                     /gene="hpt"
                     /locus_tag="PLZ75_05780"
     CDS             complement(9470..10006)
                     /gene="hpt"
                     /locus_tag="PLZ75_05780"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOS71943.1"
                     /translation="MNETIILNKRFRESIPEEAILKRIDEMADQMNHDLSGRDVLFLG
                     ILNGAFMFASEIFKRLKIDARISFVKTSSYSGTSSSGTLSRLIGLNEDIRGENVIILE
                     DIVDSGYTLESIVHEIILRGAASIGIATLLFKPEAYVKNFTLDYTGFRIPNDFVVGFG
                     LDYDGYGRNLTSVYTLVP"
BASE COUNT         2446 a         2590 c         2054 g         2950 t
ORIGIN      
        1 ctgctgattc tgaataagct gtatctcatg ctatttaccg gttattggaa ttttactaaa
       61 atagtaaatt atatttaatc cataatgagt attgctgtat ttcagttaac aatttataaa
      121 gaattccttt cagctccggg tcagtatccc cgttctgact gaatcattgt tgccagcagt
      181 ccggggtcag cattcattcc gtcaacaatt attccggccc tgttgtacca ttgttcgcgt
      241 tcagacagtt tttcccctat gaaggaaacc agctccccgt caggaatatt tttcaggagc
      301 ggtctttcgg cggatgaacc gcgaaggcgg ctggcaagct gaccgggagt cattttgaga
      361 tagaccgtaa taccggtttc acgcatgtaa tccatattat caaaatgaca gggcgttccc
      421 cctccggttg atattatgat atttgcgtca gagccaagac ctttaagtgt ttcagcctcg
      481 atcctcctga actcctcctc accgtcctcg gaaaaaatac gggcaatggt tttcccggca
      541 agctcttcta tcttatcgtc aagatccagg aactgatatc ccatccgggc tgccagcctt
      601 cttcccattg tcgatttccc gctgcccata aatccgataa tgtatatctt cctggtcccg
      661 gtcattataa ttcaagtttc atctgtgcag gatgatcctg aacaattgcc ggcaggcctg
      721 cctggtcatc ctcttcctca tcctgcccgc attcctgaac accctccttc ctgacaacag
      781 gctctatctc cagtacattg tcgaccatgt agtttgtaag tctgcgccct ttggccctgt
      841 gacttttcac accgataaat tcagcaactt caattatttc gttttcccgg ttcctgtgct
      901 tcccgccgaa acttatttcg aacctgggat attcaacttc ggtaaggctt atcagccttg
      961 agtcgggatg ttcactgatg aataaaaggg gtttttccga ttcttctatc atgaatcgtt
     1021 ttatgtaatg atatttaagt tccccgtcgt aatagattgc ggagtatact ttccccggcc
     1081 tgaatttctc aatgatgaga atattgtctt caaaatggtt tgacaggtcg aagcccgtat
     1141 ttctgaaata accgtttttg gttataacaa gtatcttgtc gtcgccggca aattcaccaa
     1201 gacagatacc cctgccatcg ccattcagcc tgaaaacggc atcatcaaac catatctttc
     1261 ttccgccaag agtggataat cccttttcct tgagtgttat tttatttacg gcatatttcg
     1321 taaggatatt ccccatggtt gtccttcctt taatggcgag ctccccgaaa tcaaattcaa
     1381 aggtaagctt cttaaggcgt gccctgggct tgtggtgtac ccttatcact tcggcctccc
     1441 cgttcggatt ggcactgaaa tatattattc tggatcccgg agtgccccgt gtcagattat
     1501 actccttgtc acgtgtaagt ccggtgatgg caaatctctt agccaggcag ggaccgtcct
     1561 tcccgtcctg gtaaacaatg ttgtaaatgg tcctttcatc gttgcggagg aaaaccgcgg
     1621 catacaggat gtcattcccc acaaatacct tgtcatcaac tttggtaatc agataagcac
     1681 catttttcct tatcacaatt atatcatcga tatcggaaca ttcacataca aattcatcct
     1741 tcttcaggga agttccgata aagccctcct tatagtttat atacaaacgg gcattatttg
     1801 ctattacctt tgcagcctga atagtatcaa agtttcttat ttcggttttc ctttccctgc
     1861 ccttgccgta ttttttcttt atctgcctga aatagttaat agtataggga ataatattat
     1921 tcaggtgatt tttaacctcc tcaagttcag cctcaatacc ctttatgtgt tcgtcagcct
     1981 tacggacatc atattttgat atgcgcttaa tccttatttc cgtaagtttt attatatcat
     2041 cctgagtaac atccctgagc agtctgtttc tgaatggttt cagtccgtca tcaattgtac
     2101 tgatgacact ctcccatgtt tcgcactctt caatattcct gtagatcttt tcttctatga
     2161 atattttttc gagtgaggac atgtgccagt cttcctgaag ttcgtttttc cgtatttcca
     2221 gttccgtttt cagtaattcc acggttctgg aagctgaatg tttcaggatc tcgctcactc
     2281 ccatgaatga aggcctgcct tctgatataa cgcaggtgtt gggagaaatt gaatattcac
     2341 aatcagtaag ggcataaagg gcatcaatgg tcttgtcagg agaaacgccc ggcatgagat
     2401 gtatctttat ttccacatct gcagaggtgt tgtcgtcgat cttccttatc ttgatcttgc
     2461 ccttttcgtt tgcctttatt atagattcga taagtgtggt ggtagtcttg ccaaagggga
     2521 tctccgtaat tacaaggctt ttcttgtcta tcttttctat tctcgccctt actttcacaa
     2581 caccgcctct ctggccgtca ttatattttg atacatcaat gaatcctccg gtcgggaaat
     2641 cgggatagat cacaaattcc ttcccctgga gatggtttat cgttgcatca ataagctcat
     2701 tgaaattatg aggcagtatt tttgaagcaa gtccaacggc aattccttcg acacccatgg
     2761 caagaagcag gggaaatttg gccggcaggc taaccggctc cctgttgcgt ccgtcatagg
     2821 attgtttcca ttcggtcgtt ttaggattaa aaatcacttc aagcgcaaac ttcgacagcc
     2881 ttgcctctat atacctgggg gcagctgccc cgtcaccggt taatatatta ccccagttgc
     2941 cctgggtttc caccagcaga tccttttgtc cgagctgtac aagggcatct ccgattgagg
     3001 catcaccatg gggatgaaac tgcattgtat gacctatgat gtttgccacc ttgttgaagc
     3061 gtccgtcatc catgcgtctc atggaatgaa ggattctccg ctgaacaggt ttcagtccgt
     3121 cgaacaggtc cggaacagcc ctttcaagaa tgacatacga ggcataatca agaaaccagt
     3181 ccctgaacat tcccggcaat gcggtaacgg aatgaagtgc tgccggggaa tattccgttt
     3241 cttccccgct ttccggataa ccgctttcaa attcaagttc ttccataatt cacaacttat
     3301 cccgccctgt atccctttaa ctgttaagcc tcaacaagat cttcttcaaa cttaaggttc
     3361 tcaattataa aatcctgtct ttccggtgtg tttttcccca tatagaattc aaggtatccg
     3421 tttaccgcgt cgtttctttt cattctgacc ggttcaagac gcatatcctt tccgatgaag
     3481 tatctgaatt catcaggaga tatttccccc aggcccttga accttgttat ttccggattg
     3541 gggccgagag tctctattgc ccttgacctt tccgcggagg tataacagta ttttgtttcc
     3601 ttcttgttgc gcaccctgaa aagtggtgtc tggagtatgt atacatggcc ctttctgacg
     3661 agatcgggga agaactggag gaagaatgtg agaagaagga gccttatatg cattccgtca
     3721 acatctgcat ctgttgcaat tacaacattg ttgtagcgca ggttttcaat cccgtcttca
     3781 atattcaggg cggcctgaag cagattgaac tcttcgttct cataaacaat tttcttggtg
     3841 agtccgaagg aattgagcgg cttgcccctg agactgaaaa ctgcctgtgt ttcaacatcc
     3901 ctcgacttgg tgattgaacc gcttgcagaa tcaccctctg taatgaatat tgttgattca
     3961 agcttgcggg gatcggcgga attgaaatgt attctgcaat cccgcagttt cttgttgtga
     4021 aggctcacct tctttgccct ttccctggca agcttctgga ttgaagaaat agccttcctc
     4081 tccttttctg aatcctgtat ctttttaaga attattctgg atgtttccgg attcttatga
     4141 aggtaatcgt caagcctttt cttaacaaac tcatttatga aattccgtat cgagggtcct
     4201 ttgggaccca tgtcctttga cccgagcttc gttttggtct gggattcaaa aaccggctcc
     4261 tccaccttga tacttattgc cgctataatc gaggatctta tatccgaagg gtcatagtcc
     4321 ttcttgtaga actcccttat ggttttcacc accgcttccc ggaaaaccgt gagatgggta
     4381 cctccctgtg tggtattctg tccgttgacg aagctgagat attcctcccc gtactgatgt
     4441 ccgtgggtaa atgcaatctc aatgtcatcc cctttcaggt gaataagggg ataaagtcct
     4501 tccctgctca tgttgtcagt gagcatgtca agcaggccgt ttctggagaa gtatttattg
     4561 ccgttgaaat ttattaccag tcccgtattc aggtaggtat aattcttcag catagtctca
     4621 acatattccg atatatagaa atagttgcca aacatgtttt catcgggctg aaatataacc
     4681 tcaaccccgt tctcttcatc agttttctcc agaggatggt ccttcagtaa gattccgtgt
     4741 tcaaactctg tcattttcac ctctccgtca cggacagatc tgattatgaa ccttgaagaa
     4801 agtgcattga ccgcttttgc cccgacaccg ttcaggccga ccgatttctt gaaggccttg
     4861 gaatcatatt tggcaccggt attcattctt gcaaccgcat cggtaacctt gcccaacggt
     4921 attccacggc cgtaatcacg cacccttacc tccctgccgg tgatggaaac atctattttc
     4981 ttcccgaatc ccatcatgaa ttcatcaatg gcattatcca taacctcttt cagcagcaca
     5041 taaattccgt catcctgtga tgagccatcc ccaagctttc ctatgtacat accgggcctg
     5101 agcctgatat gttcacgcca ttcaagcgtc ctgatatgtt cttctgaata tcctgcagcc
     5161 gtcatttagc ggaaattttg cgcaaatata gtcaattagg caagtctcct gcgggtaatt
     5221 tttcaacaat cacagggagg tgatattctc ctgaaagcgg catacaaatc atgtcaggaa
     5281 tatttatctg agtgaatgac aattgtattt tcagcgggaa tgttgataaa agattttttt
     5341 ccctccctgt ttttacagca tcagtgccgc aaccctgagc agggtttttt tctgccgcag
     5401 gtcttccccc ctgaggtcaa aatcccatat ccacagaagc actcagggaa ttcatcaggc
     5461 agtaatcagg agtgtgggtc atttttcccc ggtttccctt gtcatttcat caaaaacctc
     5521 tgtcatgcgt gtccggaatt tcacggacag ttcatcgcag tcgtccgttc cgaatgattc
     5581 cggcggaaca ggatccagaa ccctgatcat gatcctgcgg taatctccaa acaccagtcc
     5641 gtgcttcgga agtatgtcgc gggtaccggt aagcagaaca ggaaggacag gaagctgtgc
     5701 agcaatagcc agctcaaatg cacctctcct gaaaaatccg atttccccgt caaaagagcg
     5761 tgtcccttca ggaaaaatca ttattgaaat accctttttc agacaggcaa gtgattcctc
     5821 catcattttt tccttactct ccctgtttcc cctgtcaacc gttatatagc cggccatcct
     5881 gagataccat cccagaacag gcactttcat gttttcaatc tttgaaatcc atttgaaacg
     5941 gtaacgcagg cagttgagca gaagaatatc aagtatcgac tgatggtttg aaatgatgac
     6001 ataggtttgc ttccttactg ctttttccct gccctcaatc cttattttcc ataccggaat
     6061 cagatatgtt atgaccaggg atttgtaaat cagcagccag tgtacaatgg tcctgttcct
     6121 gtcaaagggc aggaccagga gccatatgac aaaaaccacg ggaaagaaca gcaccatgat
     6181 cattatccct gtaaaccaga ccagtattga cctgagaata ttcatacttt gaatttttat
     6241 accagcgctc tgcagctccg gcaatgattt gagaatgtcc ttattcaata tctgtattgt
     6301 tccggcgcat acctctcccg gtgctgctta aaaaaatttg taaatatccg ggctttccag
     6361 tctgatattt tcattcctga acaccttcat catatcttcc ggcatatgtc tgcagcccgt
     6421 cagcccttcc gctgcccttt ccagccctgt gtttcccgat attgcacatt ccctgattat
     6481 tccatcctta acggtcatcc ggcaggaaca tgattccccg ccgatctcaa actcatccgc
     6541 aaactggtat tccggcccgt aagcatagtt ccattcccag gtccggtatt ttgaatcagc
     6601 aatcagtctg atttgactct cttccccgga agtgagcctg tgcagcctgt tttcctctct
     6661 cctgaggaaa aagtcgagca acagcgacct gaactcctcc atattcaccc ttccgcccat
     6721 caggtccccg agattggcaa cccgggaagg aactgaatca accgccctgg taagatagga
     6781 agatgtattt tttcgaagcg aacgtttcag catctctgtg tcggcattga acaggagcgt
     6841 tccgtgatga aggactctct cgcggtagac atgttcggca tttcccgaaa tcttgagccc
     6901 cttcaccctg aggttgtttc tcccttcaag gacagcatcc gttcccagtg atgccaggaa
     6961 ttcaataacc ggccgcgtat atttcctgaa attcacctga tggccgctcc ggctctgaat
     7021 aatgaatgag aaattgatgt tgccccggtc gtgataaact gccccgcctc cggatatcct
     7081 cctgataacc ggaataaagt tccggctgac gaattttgta tcggcttcac ggtgtgcaac
     7141 ctgatgtttt ccgattatga ccgagggatt gttcactccg ataacaagaa aatcttcctc
     7201 tctgttcctg agcaggtatt cctcggctgc aagattgaaa cagggatcgg ttgtatcaag
     7261 gtcaatgcag atcattgcaa aatgatttca ttccggcctg atgttttttt ccgcaacaag
     7321 atacagcctg tagcagctcc accctatcag tgccccgaga agcgacccgc aaagcacatc
     7381 accgggatag tgcactccga gatagattct gctgtatccg accagcagtg cccagatgat
     7441 catgaagacc gagaaccatc tttccctgat gaaaagaaga ctcagtaccg caacattgaa
     7501 ggagtttgcc gcatgggatg atacaaatcc gtaaagtccc ccgcaatggc agttaacaat
     7561 gtgaaccatt ccctgaagcg aaggctcgtg gcagggcctg agcctctgaa acacattttt
     7621 gaacagatgc accgacgtca gctccgtaag ggtggctgcc aggatgataa aaaggagaat
     7681 aaggagggct ttcctcctgt acttcagcca tatccatatc agaacagcta aataaagagg
     7741 tacccaggtc agaattccgc tgattatcca catcagcttg tcgagaaagg gagaatatcc
     7801 tgaattcagg aaaagaaaaa tctggcgatc aaggttttca agcatgtaat cagatcagtt
     7861 ttcgttaagg actccccaca acaggtcctt cagctgattc agtcccgttc ctgtaaagga
     7921 ggacacaaat attcccggta tgtcgggcag atccctgctt agtcctgatt tgagctcatc
     7981 atccagcaga tctgttttgg aaactgccag aactcttttc ttgtcgagca gttcaggatt
     8041 atacaggctt agttcattca gcagtatttc atattccctg cgtatgtcgc cggtgtcggc
     8101 agggataacg aaaagaagga gggaatttct ttcaatatgc ctgaggaatc ttacaccaag
     8161 gcccttccct tcgtgagccc cttcaattat cccgggaata tccgccatca cgaacgattt
     8221 gttatccctg caggcaacaa taccaaggtt tggtacaagt gttgtaaacg ggtaatccgc
     8281 aattttgggc tttgcggatg aaaggaccga aagaagtgtt gattttccgg cattgggaaa
     8341 gccgaccagg cccacatcgg ccagaagctt gagttcaagg atgaaccatc cttccgaacc
     8401 tttctcaccc ggctgggcat agcgcggggt ctggtttgtc ggagacttga aatgtacatt
     8461 tccaagtcct cccctgcctc ctttcaccag tatcctttcc tcgccgtgag atgtgacttc
     8521 aagaatcatt tcccctgatt ccgcagtctt tacaacggtt cccggaggca cttctattac
     8581 cacatcctcc ccgtcggcac ctgttgactg ctgtcttccc cctgcacccc cgtgctccgc
     8641 aaatatatgc cggcggaact taaggtgaag aagggtccac atctgaccgt tccccctcag
     8701 gataacatgt cctcccctgc ctccgtcacc accatccggc cctcccttcg gcacaaattt
     8761 ctccctccgc agatgggagg agcctgcgcc gccattgcca gaacggcaga aaattttcac
     8821 ataatcaaca aagctggctg aggacattct ctttccttat tcctgattgt caggcagatc
     8881 tagttcgcaa gccggctgac atgaacactg agcctgtcaa atatctcctc tatgcttccc
     8941 attccgttga caggctggta tatgccacgt tcgcggcagt aagagattat gggttcggtc
     9001 tttcccctgt aaaccgcaat ccggttctca ataaccacgg tatccttgtc atcaggcctg
     9061 ccggacaact cagccctccc gaccagtctt ttcaccagct catcatgctc cacatccagg
     9121 acaagcatgg agtctatttt cattttcctg tcattcagca tcttttcaag agcgactgtc
     9181 tgctcgactg ttctcggaaa gccgtcaaac aggaatccgg caacattttc tgccctgtca
     9241 atctttccgg caatcatctc tatcacaacc tcatccggga ccagctcacc ctttgccatt
     9301 atggagctca ccttccttcc cagttccgtt tctgcggcaa tctcggccct cagcatatcg
     9361 cccgtggaaa gatgggccag attgaatttt tcagccagtt ttactgactg tgtgccttta
     9421 cctgatcccg gaggaccaaa aatcaaaaag ttcagcataa aataattatt taaggtacta
     9481 atgtgtatac agaagtcaga ttccggccgt atccgtcata atccagaccg aaaccgacaa
     9541 caaagtcatt cggtattctg aagccggtat aatccagcgt aaagtttttc acataggctt
     9601 ccggcttgaa cagaagggta gcgatgccga tggaggcagc cccccgaaga atgatctcat
     9661 gaactatact ttccagggta tatccgctgt caacaatatc ttcaagaatg ataacattct
     9721 cccccctgat gtcctcgtta aggccgatca gccggctcag tgtgccggaa gagcttgttc
     9781 cgctgtatga ggatgtcttc acaaacgata tccgcgcatc aatcttcagc cttttaaaga
     9841 tttccgatgc aaacatgaag gccccgttaa gaatgcccag gaaaagcaca tccctgcctg
     9901 aaagatcatg gttcatctga tccgccattt catctatcct tttgagtatt gcctcttcag
     9961 gaatcgattc cctgaatctc ttgttcagaa taattgtttc gttcatttcc gttttctcaa
    10021 ttccgggatg ctgcgaaatt
//
LOCUS       DAOSPX010000084        10015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6536_length_10015_cov_4.173695, whole genome shotgun sequence.
ACCESSION   DAOSPX010000084 DAOSPX010000000
VERSION     DAOSPX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6536_length_10015_cov_4.173695"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="PLZ75_05785"
     CDS             complement(<1..1434)
                     /locus_tag="PLZ75_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOS71944.1"
                     /translation="MSLASVSIQRPVLTSVFAIIILLFGIVGFTFLGVREFPSVDPAI
                     ITVMTSYPGANSDVIETQITEPLEQNINGIPGVRSLTSVSSQGMSRITVEFELSVDLE
                     TAANDVRDKVSQAMRLLPRDCDPPTVSKADADANPILFLTVESSKRSLLQLSEIAELT
                     LKEQLQTISGVSAVSIFGQKRYAMRLWIDPTKLAGYGLTPMDVRNTINRENVELPSGS
                     IEGTNTQLSIRTMGLMTTPQEFNDLILKQSGDNIVRFRDIGRAELGPEDIRGILKRDG
                     VPMVQIAMIPQPGSNHIDIVDEVYTRLEYIKKDLPEDVKVNVNYDNTEYIRSSIKEVQ
                     TTIYIAFFLVVIIIYFFLRNWKATLIPILVIPISLVGSFFIMYLAGFTINVLTLLALV
                     LAIGIVVDDAIVVMENIFVKVESGMEPVEAGMKGSQEIFFAIIATTITLISVFFPIVF
                     LEGITGRLFREFSIVMAGTVAISAFLAL"
     gene            complement(1452..2555)
                     /locus_tag="PLZ75_05790"
     CDS             complement(1452..2555)
                     /locus_tag="PLZ75_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS71945.1"
                     /translation="MNKRFKIIITVLLILLGSFIIFFPKLKPLFAARQSSAAPGNPGG
                     PGGPGRGPGRNMLNVSGFVIAPTTMDELIKSIGTLRPDEEVDLSFETSGKIVEIHFNE
                     GGRVKKGDLLAKINDKPLQAQLQKLQAQIKLVEEKEFRQRSLLDKDAISQESYDQIVT
                     ELQSVQADINLILARISETELRAPFDGIIGLRYLSEGSYATPSTKIARLIKMSPIKLE
                     FSIPERYADDVKIGFPVVFSVDNKNYDARVYAVDPKIDLGTRTIVLRALYPNSREELK
                     SGRYADVTLRLNQIENAVSIPSEALIPEMEGEMVYVFRKNRAEARKVSTGLRTESHIQ
                     ISDGLNFGDTLIITGILQLRQSLPVVLDTLISN"
     gene            2926..3393
                     /locus_tag="PLZ75_05795"
     CDS             2926..3393
                     /locus_tag="PLZ75_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOS71946.1"
                     /translation="MIQRIQTVFLILVTIFAGLFIVGDLFRFSGAANPEYLMNSRGIF
                     QLAQDGVLNPELNTPVLFVLSLLLPLVSLLSVFLFKNRKLQMRILLFLLFIIFLLGTL
                     TVFYGLVFASQNLLKLSPQFELFIPVLNFVLVILAYRGVKKDDDLVRSYDRLR"
     gene            complement(3423..3884)
                     /gene="pyrI"
                     /locus_tag="PLZ75_05800"
     CDS             complement(3423..3884)
                     /gene="pyrI"
                     /locus_tag="PLZ75_05800"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOS71947.1"
                     /translation="MKGGKQQLVVSAIQNGTVIDHVPAKSLFKVIQILGLDHIENQIT
                     FGTNLESKKLGHKAIIKISERYFEDNDINRIALVAPEAKLNIIKDYMVVEKKVVKVPD
                     YITAIAKCMNPKCITNLENVTTRFQVISKKNIALKCRYCEKITSGDNLKII"
     gene            complement(3895..4806)
                     /gene="pyrB"
                     /locus_tag="PLZ75_05805"
     CDS             complement(3895..4806)
                     /gene="pyrB"
                     /locus_tag="PLZ75_05805"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOS71948.1"
                     /translation="MRNRSLVSIDDFSKEEILEILDLAGKFELRPVGKLLEGKVIATL
                     FFEPSTRTRLSFESAINRLGGKIVGFADSSISSVTKGETLFDTIKMVNSYADLIVIRH
                     PFEGSARFASEVATVPVINAGDGANQHPTQTLLDLYSIRKTQGSLDNLNVFMVGDLKY
                     GRTVHSLLMAMAHWNTTFNFISPEELRVPDEFKLHLDNAGLKYYEHLDFSDIISRADI
                     IYMTRVQKERFSDPIEYEKVKNVYVLRNSMLRNTKANLRILHPLPRVNEIHTDVDSNP
                     KAYYFEQALNGVYTRQAILCTLLDLNF"
     gene            complement(4920..5534)
                     /locus_tag="PLZ75_05810"
     CDS             complement(4920..5534)
                     /locus_tag="PLZ75_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOS71949.1"
                     /translation="MKNKGKYRTIQSVSSGFYREKGSRFISFACPLKNSDEIKPIVDS
                     VKKEHHEARHNCYAWVRGVSGEKWRTFDDGEPSGTAGRPILGQIRSFGLTNILIIVSR
                     YFGGTLLGTGGLINAYRTAARSALENAIITEHVLRDRYELVFPYQSMNDVMKIIKEED
                     LEQSEHMFDLECRMTILMETSATERIISRFSRIEDLKYRYLDSD"
     gene            5645..6028
                     /locus_tag="PLZ75_05815"
     CDS             5645..6028
                     /locus_tag="PLZ75_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HOS71950.1"
                     /translation="MSVLLDEKDLQKLIVIGDRILIKPKTPQSRTKSGLFLPPGVNEN
                     EKVQIGYVLKVGPGYPIPQVQDTDEHWKNKPEEPKYVPLQPREGDQAVYLQNSAIEIE
                     FNKEKYIVVSQSAILLLLRDEGLFE"
     gene            6041..6436
                     /locus_tag="PLZ75_05820"
     CDS             6041..6436
                     /locus_tag="PLZ75_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS71951.1"
                     /translation="MHESILKGKKILIVEDDLSSRLYLNKILEKTDAILFNAGDGREA
                     LEYVENDPEIDIVLMDIQLPVMDGYTSARKIRELRPEVKLIAQTAYSFAGDKDDMNAS
                     GFDDFLIKPIYSAQLIEKLAACFKQDPEV"
     gene            complement(6481..7656)
                     /locus_tag="PLZ75_05825"
     CDS             complement(6481..7656)
                     /locus_tag="PLZ75_05825"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOS71952.1"
                     /translation="MVLTGLEQLREKLPAPLKGRKIAVLCHAPSITSDFVHITEIFRN
                     NKDCHLAAVFGPQHGLFGQTQDNMIEWEGEEDPVLNIPVYSLYGKNRKPTAAMLEGIE
                     ALVIDLQDVGARLYTYIWTVKLCIEACSEKGIPVWILDRPNPVGLLPFDGPVLKEEYF
                     TFVGGACIPLCHRMTVGEMAEWIRVKYYPSADVRVLWMKGWHRHSLYSTTGLPWVLPS
                     PNMPSLQTAVVYPGTVLFEALNVSEGRGTTIPFELTGAPFINEEKLKKELDSRHIPGC
                     RFRIHNFIPTFNKYQDEYCRGIQIHVTDHENYYPVGTALELIDSIIETSPEGSLKFNP
                     PPYEYEEKLMPFDILSGDSIMRKVLLNRGSIKNERERWKEEIEDFRKEFRNISVYPE"
     gene            7771..9528
                     /locus_tag="PLZ75_05830"
     CDS             7771..9528
                     /locus_tag="PLZ75_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOS71953.1"
                     /translation="MKYLKTRISLLILLIPILVLSCGEGTIRVSVVETTDIHGVILPY
                     DFIDMKPFDASLAHASSFFKMAAREKDAVFLLDNGDNIQGQPAVYYYNYVDTLSPHLM
                     SDVFNFLGYDAAAVGNHDIEAGHSVYDRLRTEYNFPLLAANAVDVSTGEPYFSPYALL
                     EKKGIKIAVLGLTTPFIPNWLPEELYSGMEFRDMVSTAEFWMPRIKKEEPDLIVGLFH
                     SGWNSQNTAYHPMEENGSAAVAYNVPGFDIIFTGHDHRVANETFVNREGDTLLILNGG
                     SRAQNIAVAEIEFSLKGRGKVMKKKIRGHIVETASLPPDDEFMQKFSLQGEAVNKYVS
                     RVIGNSESDISSRDSYFGSSPFVDMIHSIQLKLTGADISFAAPLSFDEVIRKGPVTVG
                     DMFKLYRFENTLYTMKMTGEEIRKYLDYSYSLWLNPMKGPGDFLLKYRLDDNGRVRII
                     NGRAWLRNQSYNFDSAAGLDYIADVSRPDGKMVIVRSLSDGRPFEKHKYYTVALNSYR
                     GNGGGGHLTRGAGIKAEELKQRLINSTERDLRHFILGEIEAGRVLAPKALKNWKIVPE
                     KWVENAVPRETELLFGKTN"
     gene            9597..>10015
                     /locus_tag="PLZ75_05835"
     CDS             9597..>10015
                     /locus_tag="PLZ75_05835"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOS71954.1"
                     /translation="MKLKRKHLILLSLGIVLTGSLLLLALRNELSSGKTKNIILFIGD
                     GMGVTHVTAGITVNKVPLNLQRFPYSGFSMTQSADNYITDSAAGGTAIACGVKTNNGM
                     IGLNPDGSVAASILEVLHENGLATGLVSTSSITHATPA"
BASE COUNT         2662 a         2216 c         2215 g         2922 t
ORIGIN      
        1 aagggcaaga aatgctgata tcgccacggt tccggccatt acaatactga actccctgaa
       61 aaggcggccc gttattccct ccaggaaaac tatgggaaaa aaaaccgaga taagagtaat
      121 ggttgttgca attatggcga aaaaaatctc ctgagaaccc ttcatcccgg cttcaacagg
      181 ctccattccc gattcaacct tcacaaaaat gttttccatc acaacgatgg catcgtcaac
      241 gacaataccg atagccagta ccagcgcaag aagcgtgagt acatttatgg taaaccctgc
      301 caggtacatt atgaaaaatg agcccacaag tgaaatggga atgacaagta tgggtataag
      361 tgtcgccttc cagttgcgga gaaagaaata aatgatgatg accacaagga agaaggcaat
      421 gtagatagta gtctgcactt cctttattga ggaacgtatg tattcggtgt tgtcataatt
      481 tacattgacc tttacatcct ccggcaggtc tttctttata tattctagcc tcgtgtagac
      541 ctcgtcaact atgtcaatat gattggaccc gggttgtggt atcatggcta tctgaaccat
      601 gggcaccccg tctcttttca atataccccg gatgtcttcc ggacccagtt ctgcccgtcc
      661 gatatccctg aaccttacaa tattgtctcc tgattgtttt agaatcagat cgttaaactc
      721 ctggggggtg gtcatcagac ccatggtcct gattgaaagc tgggtattgg ttccttcaat
      781 gcttccggac ggcagttcca cattctccct gttgatcgtg ttccttacat ccatgggcgt
      841 cagtccgtat ccagccagtt tggtcggatc gatccacagc ctcattgcat acctcttctg
      901 accgaaaatg cttacggcac ttaccccgga tattgtctga agctgctcct tcagcgtcag
      961 ttcggcaatt tcagaaagct gaagcaacga cctcttgctg ctttcgacag taaggaagag
     1021 gatggggttt gcatcggcat cagcctttga gacagtggga ggatcacagt cgcggggcag
     1081 aagcctcatt gcctgtgaca ctttatccct gacatcattt gccgcagttt caagatccac
     1141 ggatagttca aattcgacag tgatcctgct cattccctgg ctgctgacgc tggtaaggga
     1201 tctgactccg ggaataccgt tgatattctg ttcaagaggt tccgttatct gtgtttcaat
     1261 aacatctgaa ttggcacccg gatatgaagt cattaccgtg ataatagccg ggtcaacact
     1321 gggaaattct ctgaccccca gaaatgtaaa accgactatt ccgaaaagca gaatgataat
     1381 tgcaaatact gatgtaagaa ccggtctttg tatgctgact gatgctaggc tcatgcttta
     1441 attttaaatc attaattgga aatcagtgtg tccagcacca caggcagact ctgccgcagt
     1501 tgcaggatac ctgttattat aagagtatca ccgaaattca gtccgtctga tatctgaata
     1561 tgggattccg ttctcaatcc ggtactgact tttctggctt cagccctgtt ttttctgaat
     1621 acatacacca tttcaccttc catttccgga atcagggctt cacttggaat tgatacggca
     1681 ttttctatct ggttcagtct cagggttaca tcagcatatc ttcctgattt gagctcttcc
     1741 ctcgaattgg gatacagggc cctgagtaca atggttctgg tgccgaggtc tattttcgga
     1801 tcaactgcat aaacccgggc atcatagttt ttattgtcaa ccgaaaacac aacagggaag
     1861 ccgattttca catcatcggc gtacctttcc ggaattgaaa actccagttt gatgggactc
     1921 attttaatca ggcgggcgat ttttgtggaa ggagtcgcgt aactcccctc gctgagatat
     1981 ctcaggccaa tgattccgtc gaaaggtgca cgcagttccg tttcggatat ccttgccagg
     2041 attagattga tatctgcctg aacactctga agttcggtaa caatctggtc ataactctcc
     2101 tggcttatcg catccttgtc gagcaggctg cgctgccgga attctttttc ctcaaccagt
     2161 ttaatctggg cctgcagttt ttgaagctgg gcctgaaggg gcttgtcatt tatctttgcc
     2221 aggagatcgc cttttttcac cctgcctcct tcattgaaat gaatctccac aatttttccg
     2281 gatgtttcaa atgaaagatc aacctcctcg tccggcctta aagtcccgat actcttaatc
     2341 agttcgtcca tggttgtcgg ggcaataaca aaaccgctca cgttaagcat gttacgaccc
     2401 ggtcccctgc ctggtccgcc cggcccgccc ggatttcccg gtgctgcgga actctgtctt
     2461 gccgcgaaca ggggtttgag tttcgggaaa aatattatga aggaacccag caggatcagt
     2521 aagacagtga ttatgatttt aaaacgttta ttcatatcgg gtaattatgc cgttttggtt
     2581 aataatgttg acagcgttga aatcctgctg tatcctgaat tatctgttta caaatttctt
     2641 aatgcccttg tttaaacata tttaataaca aatagttcaa tggcacggac acaggccccg
     2701 gtgccgggtt gcatatagtg gatgagtcat cagttcaaac ctttataagc agtgaaagga
     2761 ctgacaaagg tagatatttt gttatttaat tattaattcc taatatgtta atattgttaa
     2821 atattcctaa aatccggatt atgcttaagt atgtaattac ctgccggacc aggacgtttt
     2881 ttaaataaaa caaattattt ttacccttta ctaattttat gaagcatgat ccagcgtatt
     2941 caaacggttt ttctgatcct ggtaaccatt tttgcaggat tgttcattgt gggcgattta
     3001 ttcagattca gcggcgccgc gaatccggaa tacctgatga attccagggg aatttttcag
     3061 cttgctcagg acggggtttt aaatccggaa ctgaacactc ccgtcctgtt tgtattatcc
     3121 ctgctgctgc ctcttgtttc gctcctttct gtctttttgt ttaaaaacag aaagcttcag
     3181 atgagaatcc tgctgttcct tctgtttatt atttttttgc ttggaaccct gacagttttt
     3241 tacggcctcg tttttgcgag tcagaatctc ctgaaactga gtccgcaatt tgaactgttc
     3301 attcccgtac tcaactttgt tctggttatt ctggcttaca ggggagtgaa aaaggatgat
     3361 gaccttgtaa ggtcatatga ccgcctgcgt tagaatacgg ccgctccggt catgataccc
     3421 ggtcagataa tcttcaggtt gtccccgctt gttatttttt cacagtagcg gcacttgagg
     3481 gcaatatttt tctttgaaat cacctgaaag cgggtcgtta cattttcaag gtttgtaatg
     3541 catttgggat tcatgcattt tgcaatggca gttatatagt caggaacctt caccaccttc
     3601 ttttctacga ccatatagtc ttttatgata ttaagctttg cctcaggggc cacaagggct
     3661 atgcggttaa tatcattatc ctcgaaatat ctttcagaaa tttttataat cgctttgtgt
     3721 ccgagctttt tgctttcaag atttgttccg aatgtgatct ggttttctat atgatccagg
     3781 ccaagtatct gtattacctt gaaaaggctt ttcgcgggca catggtctat gactgtgccg
     3841 ttctggattg cgcttaccac aagctgctgt tttcctcctt tcatctttgt gcctttagaa
     3901 attcaaatca agtaaggtgc ataatatggc ctgccgggtg tatactccgt tgagtgcctg
     3961 ttcaaaatag taagctttcg gattgctgtc aacgtcagta tgaatttcat tgacccgtgg
     4021 cagaggatgg agaattctca gattcgcttt cgtgttccgc agcatggaat tcctgagaac
     4081 atatacattc tttaccttct catattcaat gggatcggaa aatctttcct tctggactct
     4141 tgtcatgtaa atgatatcag ccctggaaat tatatcggaa aagtcgaggt gttcataata
     4201 cttaagtcct gcattatcaa ggtggagttt aaactcgtcg ggcactctca attcctccgg
     4261 tgagatgaag ttgaatgtgg tgttccagtg agccattgcc ataagaaggg aatgaacagt
     4321 ccggccgtat ttgagatcgc cgaccatgaa aacattgagg ttgtcaaggc ttccctgcgt
     4381 ttttctgatt gaatagagat ccagaagggt ctgggtcgga tgctggttgg ctccgtctcc
     4441 ggcattgatc accggtacag tggcaacctc gctcgcaaat ctcgcactgc cttcaaacgg
     4501 atgcctgatg acaatcagat cagcataact gttgaccatc tttatggtgt caaacagggt
     4561 ctcacctttt gttacactgg agattgatga gtcggcaaaa ccgactatct ttcctcccag
     4621 acggtttatg gcactctcaa aacttagccg ggttctggtg gatggctcaa aaaacagagt
     4681 cgctatcacc ttgccttcaa gcagctttcc cacaggccgt agttcaaatt tacctgcaag
     4741 gtcaagaatt tcaagaattt cttctttact gaaatcatct attgatacca agcttctgtt
     4801 tctcattcat ttatgacttt tagaaagcgt atcgcgttac atatcaaatt taaagaactt
     4861 taatgactga ctgttaagtg ttaataattt atttaaaact ttaaacggag ctgtccggat
     4921 taatcgctat caagatacct gtatttcaga tcttcaattc ttgaaaagcg tgaaattatt
     4981 ctttctgtgg ccgaagtttc catgaggatt gtcatcctgc attccaggtc gaacatatgc
     5041 tccgactgtt ccagatcctc ttccttaatt atcttcatta catcattcat tgactgatag
     5101 gggaaaacca gctcatatct gtcccttaga acatgctcgg ttattatggc attttccagg
     5161 gctgaccggg ctgcagtccg gtaagcattg ataagtcctc ctgttccaag aagagttccc
     5221 ccgaaatatc tggaaacgat gataaggata tttgtgagcc cgaaggatct tatctggccg
     5281 agaatgggac ggcctgcggt accggaaggt tctccgtcgt caaatgttct ccatttttcg
     5341 cccgagactc cccttaccca cgcatagcaa ttatgcctgg cttcatgatg ttccttttta
     5401 accgaatcca caatcggctt tatctcatct gaatttttca gggggcaggc gaaagaaatg
     5461 aatcggcttc ccttttccct gtagaatcct gaagaaactg attgtatggt ccggtatttc
     5521 cccttgtttt tcatcggctg tacaaggcat gcaaaataca ctttagctgc aatattacct
     5581 aattccgtat ttcagttcaa ggttttcata aattttggta aatttgcatc tgactaaaaa
     5641 tgacatgagc gtattgttag acgaaaaaga cctgcagaaa cttattgtta ttggcgacag
     5701 aatccttatc aagccaaaga cacctcagtc caggacaaaa agcggccttt ttctgccgcc
     5761 cggggttaat gaaaacgaga aggttcagat cggatatgtc ctgaaggtag gacccggata
     5821 tcccattccc caggttcagg atacagatga gcactggaag aacaaaccgg aagaaccaaa
     5881 atacgttccc ctacagccca gggaaggcga ccaggcagta tatctgcaga acagtgccat
     5941 tgaaatagaa ttcaacaagg aaaaatatat agtggtgtca cagtccgcaa ttttgttgct
     6001 gttgcgtgat gaaggcctgt ttgaataatt ggtttaaaaa atgcatgagt caatcctgaa
     6061 agggaaaaaa atcctgattg tggaagatga tctgtcaagc aggttgtatc ttaataaaat
     6121 tctggagaaa acggatgcga tactgttcaa tgccggtgac ggcagggaag cactggaata
     6181 tgttgaaaat gatccggaaa ttgacatagt ccttatggat atccagcttc ctgtaatgga
     6241 tggatatact tcagccagaa aaataaggga acttcgcccg gaagtgaagc ttattgccca
     6301 gactgcatac agctttgcag gggacaagga tgatatgaat gcatcaggct tcgatgattt
     6361 cctgataaag ccgatttatt cggcacaact gatagagaaa ttagctgcct gttttaaaca
     6421 ggatccggaa gtttaaaggg aatcagtgtc tttacagaat ccgtaaaagc aggaaacggc
     6481 ctattccgga tatacggaaa tatttctgaa ttcctttctg aaatcttcaa tttcttcttt
     6541 ccacctttcc ctttcgtttt taatagatcc cctgttcagt aaaactttcc tcattattga
     6601 atctcccgac aatatgtcga agggcatgag tttttcctca tattcgtaag gagggggatt
     6661 gaatttcagg cttccttcag gagaggtttc aattattgaa tcgatcagtt caagtgcagt
     6721 acccaccgga taataattct catgatccgt gacatgtatc tgtatgcccc tgcaatattc
     6781 atcctggtat ttgttgaagg tgggaatgaa attgtgtatc ctgaaacggc aaccgggaat
     6841 gtgtctgctg tccagctcct ttttcagttt ttcctcattg atgaagggag ctccggtcag
     6901 ctcaaaggga atcgtggtcc cccttccttc ggaaacattc agagcttcga acagtacggt
     6961 ccccggatat accactgctg tctgaagtga aggcatgttg ggtgacggca atacccacgg
     7021 aagcccggtc gtgctgtaaa ggctgtggcg gtgccagcct ttcatccata aaaccctgac
     7081 atcagccgaa ggataatact taactctgat ccattcggcc atttccccta cagtcattct
     7141 gtggcacagg ggaatacagg ctccgccaac aaaagtaaaa tattcttcct tcagcaccgg
     7201 accgtcaaaa ggaagtaatc ccacaggatt gggccggtcg agtatccata ccggtattcc
     7261 cttttcactg catgcctcaa tacataattt caccgtccat atataggtat agagccttgc
     7321 acccacatcc tgaagatcta tgacaagggc ttctattccc tccagcatgg ctgctgtcgg
     7381 tttacggttt tttccgtaaa ggctgtaaac cggaatattg agaacagggt cctcttctcc
     7441 ttcccattca atcatattgt cctgtgtctg tccgaacagg ccgtgctgcg gcccgaacac
     7501 agcagccaga tgacagtctt tattgttccg gaagatctcc gtgatgtgaa caaaatcgct
     7561 ggtaatgctt ggagcatgac ataaaaccgc aatcttcctt cccttaagag gggcgggtaa
     7621 tttctccctc agctgttcca gacctgtaag taccataaaa aataaaagat tacatataaa
     7681 tttcttaaat gtacatgaaa aattcctgtt aagaatattc tttcctagtt ttatgggctt
     7741 tattattcat tcttcagaaa gaaaaggacc atgaaatatc ttaaaaccag aatttccctg
     7801 ttgattcttc tgattcccat actggtattg tcctgcgggg aagggacaat ccgggtttca
     7861 gtagttgaga caaccgatat ccacggggtt atactgcctt atgattttat tgatatgaaa
     7921 ccctttgatg catcactggc acatgcctca tcatttttta aaatggcggc cagggagaaa
     7981 gatgcggttt ttcttcttga caatggtgat aatattcagg gacagccggc tgtgtattac
     8041 tataattacg tggatactct ttctccgcac ctgatgtctg atgtattcaa ctttctcggt
     8101 tatgatgcgg ctgctgtcgg gaaccatgac attgaagcgg gacattccgt ctatgaccgt
     8161 ctcaggacgg aatacaattt tccgcttctt gctgcaaatg cagttgatgt aagtacgggt
     8221 gaaccctatt tcagccctta tgcattactt gaaaaaaaag gaattaaaat cgcagtgctc
     8281 ggacttacga caccttttat cccgaactgg ctgccggagg aactttattc cgggatggag
     8341 ttcagggata tggtcagcac ggcggaattc tggatgccaa ggataaaaaa ggaagaaccg
     8401 gacctgatag tcggactttt ccattcagga tggaacagtc agaatacggc ttatcatcct
     8461 atggaagaaa acggatctgc ggcagttgcc tacaatgttc cgggcttcga cattattttt
     8521 acgggacatg atcaccgggt ggccaatgag acctttgtaa acagggaagg ggatactttg
     8581 ctgatattaa acggaggaag cagggcacag aatattgcag ttgctgaaat cgaattttca
     8641 cttaagggca gggggaaagt gatgaaaaaa aagatacggg gtcatattgt agaaactgca
     8701 tctttgcccc ctgatgatga attcatgcaa aaattcagtt tgcaggggga agctgtcaat
     8761 aagtatgtga gcagggttat cggcaattct gaatcagata tttcatcccg tgattcctat
     8821 ttcggctcat caccatttgt ggacatgatt cattccatcc agctgaaact cacaggtgcc
     8881 gatatttctt ttgccgcccc gctttccttt gatgaagtga tccgtaaggg acccgttacg
     8941 gttggtgaca tgttcaagct ctacaggttc gaaaatacgc tctatacaat gaagatgacc
     9001 ggggaagaga tacggaaata ccttgattac agttattccc tgtggctcaa tcccatgaaa
     9061 ggcccggggg attttctttt aaagtacagg cttgacgata acggcagggt aagaattatt
     9121 aacggacgtg catggctgag aaaccagtcc tataacttcg actctgccgc aggtcttgat
     9181 tatattgctg atgtgagcag gcccgacggg aaaatggtaa ttgtcagatc cctgagtgat
     9241 ggcaggccct tcgaaaagca caaatactac actgttgcac ttaattccta ccgtgggaac
     9301 ggcggcgggg gccacctgac aagaggtgcc ggaatcaaag cggaagaact gaaacaaagg
     9361 ctcataaatt ccacggaaag ggatcttcgc catttcattc tgggagagat tgaagccgga
     9421 agggtcttag cgcccaaagc attgaaaaac tggaaaatag ttcctgaaaa atgggtggaa
     9481 aacgctgttc cgcgcgaaac agagctcctg ttcggcaaaa caaattgacc ggactgatgt
     9541 tataatggct aaacaggaaa taattttatt aatatcaata ctgaaaattt tataaaatga
     9601 aactaaaaag gaaacatctg attttattgt ctctgggtat tgttctgacc ggttcgctgc
     9661 ttctgcttgc ccttagaaat gaactgtcct ccggaaagac taaaaatatt atactgttta
     9721 ttggtgacgg catgggtgtg actcacgtca ctgccggaat cacagtgaac aaagtccccc
     9781 tgaaccttca gaggttcccc tattcaggct tcagcatgac ccagtcggct gataattaca
     9841 taactgattc ggcagccggt ggtactgcca ttgcatgcgg ggtgaagaca aacaatggaa
     9901 tgatcggact taatcctgat ggttcggttg cagcttcaat ccttgaggtt ctgcatgaaa
     9961 atggactggc aacgggcctg gttagcacca gttcaattac acatgccacg ccggc
//
LOCUS       DAOSPX010000085         9980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6564_length_9980_cov_5.033753, whole genome shotgun sequence.
ACCESSION   DAOSPX010000085 DAOSPX010000000
VERSION     DAOSPX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9980
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6564_length_9980_cov_5.033753"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1547
                     /gene="ltrA"
                     /locus_tag="PLZ75_05840"
     CDS             144..1547
                     /gene="ltrA"
                     /locus_tag="PLZ75_05840"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HOS71955.1"
                     /translation="MKGRMQKISTPSDNCPRKDRTASESYAGVQTFIGITENNLIEVR
                     FGGDDLLERIVSPTNLNRAYKQVMSNRGSGGVDRMRTEELLPWLHRHKESLINSLMDG
                     TYRPCPVLRVEIPKGNGQTRPLGIPTVVDRLVQQSISQVLTPLYEVEFSETSYGFRPK
                     RGCHDGLRKAQSHINSGYKYAVDLDLEKFFDTVSHSRLIEVLSRKIRDGRVISLIHKY
                     LHAGVQVGDRFGESLSGVSQGGPLSPLLGNIMLDELDKELERRGHPFVRYADDCMIFC
                     RSKRAAERTRDSIVGFIENRLHLRVNREKTKAGYIIGMKFLGYSFYVYSGKCRLSVHP
                     KSLDKFKTKLKELTGRSNGMGYAQRKESLRYFIMGWLEYFKLADMKSKLQEMDMWYRR
                     RLRMCIWKNWKRVRSRFRNLIRCGIEKRKAWEWANTRKGYWHISNSWILSRALNNDNL
                     RRANYPLLMDCYRKVVS"
     gene            complement(1776..1849)
                     /locus_tag="PLZ75_05845"
     tRNA            complement(1776..1849)
                     /locus_tag="PLZ75_05845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1813..1815),aa:Ile,seq:cat)
     gene            complement(2018..3235)
                     /locus_tag="PLZ75_05850"
     CDS             complement(2018..3235)
                     /locus_tag="PLZ75_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS71956.1"
                     /translation="MQNWKKVFGIIWSGQLISTLSSSIVGYAVVFWLSIETKSAGVLA
                     LATIAALLPQMIFGPFTGVFVDRWDRRVTMIAADLFIAVCTFLMALMFLSGNVKISLV
                     YVLLVMRSLGSAFHVPAMQASVPLLAPENELMRISGINQVIQSVSVIAGPALAALLIT
                     VLNMVWVLMIDVAGALVAVGSLMLVRIPNPPRKADAPGPHILRELKEGLAEIYYHRGL
                     LWLFIIIVLAHFFIMPVAALFPLMTISHFNGGTYQMSIVEICWGVGMLAGGALLGIKK
                     LKIPEIIIINSTFLVTGLTFLLSGILPETGFHIFAGLTFAGGISMALYSASFMVIIQT
                     NVDMAVMGRVFSLYGSLTLLPSIFGLLQTGYVADAIGVPNAFIISGMAIIMIAVWAFF
                     IPALRSMASGKLT"
     gene            complement(3269..4609)
                     /locus_tag="PLZ75_05855"
     CDS             complement(3269..4609)
                     /locus_tag="PLZ75_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOS71957.1"
                     /translation="MEKGFFMPILLLLLCQPAVAQDSDSFFKFDLLLRYRFELWDGFN
                     AKNYGDDSPDAVGNLNDKFLLQRIIPGIIYRRGKIKAEFHMQDSRAFGWSLSRKNYPD
                     LYKVGNPLSDNSFYVMNPQEEYFEIYDLFVEYRGLLKNLSVKAGRQKIFYGDWRIFEM
                     SQWGNTGRYTWDAIKISYRKDDEYIDVFGGGTKIHDPEKTHVPFTHLEYLGGGIYSHK
                     KLTSWLDAEPFYALKIPGTADYIRTRSLNRNWAGIKFLSPEDKRFSYDVLYAREFGSE
                     AGQRIKAHGFGLRTTCRLDGLPAAPELSLAYYYASGDKPGDDIIQTFDPVYGSRARFH
                     GFMGFVSWSNLSNPQFSLKLSGSGKRMYVELKHMLFYIPEPENCTFLNTMKLKDGCNH
                     LGNETNLYFRHQPNKHWQLTGILGYFTPGDIEQISLKEPENAFWMGLQVQYSLN"
     gene            complement(4724..5005)
                     /locus_tag="PLZ75_05860"
     CDS             complement(4724..5005)
                     /locus_tag="PLZ75_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71958.1"
                     /translation="MNINLVASSVKDIIVLSFGNVLVLVDKTVNSIVIISSIVLLSIG
                     LLIFIFLLFRKKKEFTAFRAIGLFFMLVYLGFFLTIIHSNGRISYSPAK"
     gene            complement(5443..6192)
                     /gene="mtnP"
                     /locus_tag="PLZ75_05865"
     CDS             complement(5443..6192)
                     /gene="mtnP"
                     /locus_tag="PLZ75_05865"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOS71959.1"
                     /translation="MVKIGIIGGSGLEDPEILDSPEIINISNKYGSPASPLVCGKIGN
                     TETVILSRHGKDHTIPPSAVNNRANISALADAGCTHIISTTACGSLREEIQRGDIIIP
                     DQFIDFTRHRYITFFDEFEPGNMGHTPMADPFDRHLRSLLISAARKLRLPVHDKGVMI
                     TIEGPRFSTRAESHLFRMWGADIINMSVAPEAILANEIKIPYATIAMSTDYDSWKKDE
                     QPVTWEEVLKVFNANVHNVLSLLREVIPLVN"
     gene            complement(6192..6791)
                     /locus_tag="PLZ75_05870"
     CDS             complement(6192..6791)
                     /locus_tag="PLZ75_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOS71960.1"
                     /translation="MEGVVKFNCHWTQCGPVISDDQYEIINYWREILYNMDLIGAYEN
                     GVGFGNMSMRIRGGNQFVITGSATGEIPDLEPGHYVKVISFNIDDNAVQCAGPLKASS
                     ESLTHAAIYLADPDVNAIVHVHSIALWNEIIYKVPTTNPAMEYGTTGLAKDIFRLFSD
                     SDVFEKRIIVMAGDRAGILTFGQDMDEAVNILMRYLGKE"
     gene            complement(6796..7905)
                     /gene="mtnA"
                     /locus_tag="PLZ75_05875"
     CDS             complement(6796..7905)
                     /gene="mtnA"
                     /locus_tag="PLZ75_05875"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOS71961.1"
                     /translation="MLAGTKNYQGIWLDESDPSVVRVIDQNRLPFFFETRELRSVEDV
                     YTAISTMTVRGAPLIGAAAAFGIYLATFELSRNADHVHQLENAARYLVSCRPTAVNLS
                     AALNFVMDRIRNNPADSSLRETALKAALDLCEQEKENCRLIGINGLPLIEDAGKRKNG
                     NPVNILTHCNAGWLASIDYGTALAPVYLAHESGIPVHVWVDETRPRNQGSRLTAWELD
                     QAGVPCTLIADNTGGHLMQHGMVDLVIVGSDRTTIQGDVANKIGTYLKALAAYDNNIP
                     FYSAFPSTTLDFRLNDGLKEIPVEERDEEELVFIEGFNGETIEKIKICPDNVRGANYG
                     FDITPARLIAGLITERGICRPSEKEIRNMFSDKIK"
     gene            8126..8707
                     /locus_tag="PLZ75_05880"
     CDS             8126..8707
                     /locus_tag="PLZ75_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOS71962.1"
                     /translation="MVNKEEYRKRIIKISGSIFSRYGFKKTSMDEIARALKMGKSSIY
                     YYFNGKEEIFKAVVMNEANILRNELTKTIKAVDSPTEKLRNYVFVRMKSFEKLSNYYN
                     AIFDKNLDHFDFIESVRTKYDREELAILRLILYDGARKKVFNVPNSEYTALAVQTALK
                     GLEVPLFWKRKEIDIENRLNGILDILFHGILAK"
     gene            complement(8797..9609)
                     /gene="lgt"
                     /locus_tag="PLZ75_05885"
     CDS             complement(8797..9609)
                     /gene="lgt"
                     /locus_tag="PLZ75_05885"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOS71963.1"
                     /translation="MHFLVIPWDVNPEIFRVGSFAIRWYGLLFASGFLFGYIIMNRIF
                     RNENLPEASLDRLTIYMAVGVIAGARLGHCLFYEPGYYLSHPGEILKIWHGGLASHGA
                     AIGILIAIWLFVRKERKDYAWALDRIAIVVALAGFFIRIGNLMNSEIYGVETTLPWGF
                     VFLRNGENAPKHPTQIYEALAYLMIFILLYRLYWRKKGEHYQGTLISLAMILIFTARF
                     FLEFLKEDQVDFEQGMALNMGQLLSIPFVVAGCIWLWHSLKNKKTATIKRKG"
BASE COUNT         2790 a         2242 c         2309 g         2639 t
ORIGIN      
        1 gagtaaagct tgccgtgaga agtcagcaga agccatagta gtacggatac aagcctgccc
       61 cggattaaaa cggaatagca ggaagtctta cgaagtacga agggctgaac cttatttaaa
      121 tgggagtaaa tgaatgttac ccgatgaagg gaagaatgca gaaaatatca acaccaagtg
      181 ataactgccc gcggaaggat aggacggcat ccgaaagcta tgcgggagtg cagactttca
      241 ttgggataac agaaaacaac ctcattgaag tacgctttgg cggggatgac cttttggaac
      301 ggatcgtttc tcccacgaac cttaacaggg cctataagca ggtcatgtct aacaggggta
      361 gcggaggagt tgaccggatg aggacggaag aattacttcc atggctacac cgccacaagg
      421 agtccctgat aaacagttta atggatggta cgtaccgtcc ctgtccggtt ctccgggtag
      481 agataccgaa agggaacggt cagacccgtc ctctaggtat cccgacggtt gtcgaccgtc
      541 tggttcaaca atccatatcc caggttctca caccgctgta tgaagtggag ttcagcgaaa
      601 ccagttatgg ttttcgcccc aaacgtggtt gccatgatgg ccttcggaaa gcgcagtcgc
      661 acatcaactc gggctacaag tatgccgttg accttgatct ggagaagttt ttcgacacgg
      721 tcagtcacag tcgcctgata gaagtcctgt cacggaagat acgggacggg agggtgattt
      781 ccctgatcca caagtatctc catgcaggag ttcaggtggg tgaccgcttc ggggagagcc
      841 tttcgggcgt gtcccagggc ggtccgttaa gtccgttgtt aggcaacatc atgctcgacg
      901 agctggataa ggagctggaa agacgaggac acccgtttgt ccgttatgcg gatgattgca
      961 tgatcttctg caggagcaaa cgtgcggcag aaaggacacg tgatagcatc gtcggtttca
     1021 tagagaacag gctccacttg cgggtcaacc gtgagaaaac gaaagccggc tatataatag
     1081 gaatgaaatt cctcgggtat agtttctatg tttacagtgg caaatgccgc ctgtcagtcc
     1141 accccaaaag ccttgacaaa tttaaaacaa aactgaaaga gctgacggga cggagcaatg
     1201 gcatggggta tgcacagcga aaagaatcgc ttcgctactt tatcatgggt tggcttgaat
     1261 acttcaagct ggctgacatg aagagtaagc tccaagagat ggacatgtgg tatcgccggc
     1321 ggttgcggat gtgtatctgg aagaactgga agagggtacg aagccggttc aggaatctta
     1381 taagatgcgg gatagagaag aggaaagcat gggaatgggc aaatacccgc aagggttatt
     1441 ggcacatatc caacagctgg atactatcac gggcactgaa taatgacaac ctgagacggg
     1501 caaattatcc cttgttaatg gactgttacc gcaaagtcgt atcgtaagta aggaaccgcc
     1561 gcctacggaa ccgtacggac tggtggtgtg agaggtcgga aaatgaaata ggaggaaacc
     1621 tatttcattt tcctcctact cgattgggcc tagctgggat cagggccgag cgttaaaaat
     1681 cacgccggtg gcgtgattta gcgatggagc cagactgccg cggtggctcg atcccggcgt
     1741 gaaatggaaa aggcagccaa atggctgcct gcgtgtgggc ccagttggga tcgaaccaac
     1801 gaccccctga ttatgagtca ggtgctctaa ccgtctgagc tatgggccct tttattttta
     1861 ccatttccgg ttttgccggt cactcttacc acccttcagg atcctgggac gaaagggctg
     1921 tgtatggccc gattccgttc aggcgccggc aaacagaaaa cggactgcaa tattaaccaa
     1981 ttgttataaa aaatccaaaa ggccttccga aaaatcttta tgtcaacttt cctgatgcca
     2041 tggaccttaa tgccggaata aagaatgccc atacagcaat cattattatt gccattcctg
     2101 atatgatgaa tgcattcgga actccaatgg catccgccac ataacctgtc tggagcagtc
     2161 cgaatatcga cgggagaagc gtcaggctgc cgtagaggga gaatactctt cccatcactg
     2221 ccatgtcaac attagtctgg ataataacca tgaatgatgc agaatacaga gccatgctga
     2281 ttccgccggc aaaagtgaga ccggcaaaga tatggaagcc tgtttcagga aggattccgg
     2341 aaagaagaaa agtaagcccg gttaccagga aggttgaatt tatgattatg atttccggga
     2401 ttttcagttt ttttattccc agcaatgctc cgccggcaag catacccact ccccagcata
     2461 tttccacaat actcatctga tatgttcccc cgttgaaatg acttattgtc atcaggggga
     2521 aaagtgcagc caccggcatg ataaagaaat gggcaagaac aattatgata aacagccaaa
     2581 gcagtccgcg gtgataataa atttctgcca gtccctcttt cagttctctc aggatatgtg
     2641 gaccgggtgc gtctgctttc cggggaggat tgggtatgcg gacaagcata aggctgccta
     2701 cggctaccag ggctccagca acatcaatca tcagtaccca taccatgttg aggacggtta
     2761 tcagaagggc tgccagggcc ggtccggcta ttacacttac tgactggata acctggttga
     2821 tgccggatat tctcatcagt tcattttccg gggcgagaag tggaacggaa gcctgcatgg
     2881 cgggaacatg gaaggcactt ccaagggaac gcattacaag gagaacgtat acaagcgata
     2941 tcttaacatt gccgctcagg aacatcaggg ccatcaggaa tgtgcagaca gctataaaga
     3001 gatccgccgc gatcattgta accctcctgt cccagcggtc aacgaatact cctgtaaaag
     3061 gtccaaaaat catctggggc aaaagagcgg caatggttgc cagggcgagc actccggccg
     3121 actttgtttc gatactgagc cagaatacaa cagcataacc tacaatggaa ctgcttaatg
     3181 tggaaatcag ttgtccgctc cagataatgc cgaatacttt tttccagttt tgcatgtaag
     3241 gaattattac cagattatat cattccggtc agttcaatga gtattgtacc tgaagcccca
     3301 tccagaaagc attttcaggt tcttttaaac tgatctgttc aatatctccc ggtgtaaaat
     3361 atccaagaat gccggtaagc tgccagtgtt tattaggctg atgcctgaaa tacaggttgg
     3421 tttcattacc aagatgatta cagccatcct tcagtttcat ggtattcaga aaagtacagt
     3481 tctcaggttc gggaatatag aaaagcatgt gtttgagttc aacatacatc ctctttccgc
     3541 tgccggaaag cttcagggag aattgcgggt tgctgagatt ggaccaggaa acgaatccca
     3601 taaatccgtg gaatctggcc cgcgacccgt aaaccggatc aaaagtctga attatatcat
     3661 cccccggctt atcaccggag gcataataat aggccagact cagttcgggt gctgccggca
     3721 ggccgtcaag cctgcatgtc gtccttaatc cgaatccgtg agccttaatc cgctgtccgg
     3781 cttcactgcc gaattcacgt gcataaagaa catcatacga gaatcttttg tcttccggac
     3841 ttaagaattt tatacccgcc cagttcctgt ttaatgaccg ggtcctgata tagtcggcgg
     3901 tgccggggat cttcagggca tagaaaggtt ctgcatcaag ccaggaagtc agctttttat
     3961 gtgaatatat accacctccc aggtattcca gatgagtgaa gggcacgtgg gttttttcgg
     4021 gatcatgtat ttttgttcct cctccgaaaa catcaatata ttcatcatct tttctgtagg
     4081 aaatttttat tgcatcccag gtatatcttc cggtatttcc ccactgtgac atttcaaaga
     4141 ttctccagtc gccgtaaaaa atcttctggc ggcctgcctt taccgacaga ttcttcagta
     4201 atcccctgta ctcgacgaaa agatcatata tttcgaaata ttcttcctgg ggattcatta
     4261 cgtaaaatga attatccgac aaggggttgc cgaccttata cagatccggg tagttcttac
     4321 ggctcaggga ccatccgaag gctcttgaat cctgcatatg gaattctgct tttattttac
     4381 ccctcctgta tattatcccc ggaatgatcc tctggagaag gaatttatca ttcagattac
     4441 cgacagcgtc aggactgtca tccccgtagt tctttgcatt gaagccatcc cataattcga
     4501 agcggtaacg aaggaggaga tcaaatttaa aaaatgaatc agaatcctga gcaacagcag
     4561 gctggcataa taacaggagc agtatcggca tgaagaaccc tttttccatc agctttttat
     4621 gaaaaatcag ggagttaaaa tacaacatta acccggaaaa aagctggatt cagttcattg
     4681 aattcttctg gtaatagtat aacgacctgt tattttgcca aagttatttt gcaggagaat
     4741 aggatattct gccgtttgag tgaattattg tcaaaaagaa gccaagatat acaagcatga
     4801 aaaacagacc gatggccctg aatgcagtaa attctttttt ctttctgaac aacaggaaaa
     4861 taaatattaa caggccgata gacagtaaaa cgattgaact gattatcacg atcgaattaa
     4921 cagttttgtc aaccaggact aacacgtttc cgaaggacag tacgataatg tccttcacac
     4981 ttgaggcaac cagatttata ttcatccgga tcgctgatga cagctggttt atcagcatgg
     5041 gcagggtatc atcaagaatc ccttttttga taccaaactt ttaataccac aaccgttcag
     5101 caatataaga gtgctgaaca ggacaaaaac aagaacagct ttttcatccc ggatagaatt
     5161 aagtatgact ggtattattg aattgttatt caaacaaata ccttaaaatc atttatataa
     5221 ttctaaaatt atataacgat taatcaatca gactttttgg ttattgtatg gtgtatactg
     5281 aacggtttgt taatagtgta gaaaaattca ggatcaagtc acttatataa aatttgcatc
     5341 aaaaataaag ggaagtcaaa ttttttttca ggggctgtct gtcatttccc ggcctcctgt
     5401 tttcagtgtc gttccaatca tccggtctcc ggcccgctcc cattaattta caaggggaat
     5461 aacctcgcgc agaaggctga ggacattatg gacatttgca ttaaaaactt tcaggacttc
     5521 ttcccatgtg accggctgtt catctttttt ccaggagtca tagtcggtgc tcattgctat
     5581 tgtggcatag ggtattttga tttcgtttgc cagaatggct tccggggcaa ccgacatgtt
     5641 gatgatatcg gcaccccaca tcctgaaaag gtgagactcg gcccttgttg agaacctggg
     5701 gccttcaatg gtgatcatta cacccttgtc atggacagga agccgcagct tccgggctgc
     5761 ggatatcagt aatgacctca gatgcctgtc gaagggatca gccattgggg tatgacccat
     5821 attaccgggt tcaaactcgt cgaagaatgt tatatagcga tgtcttgtaa aatctatgaa
     5881 ctgatcggga atgataatat ctccgcgctg tatttcctcc ctgaggcttc cgcatgctgt
     5941 tgtactgatg atatgcgtgc agccggcatc agccagggct gaaatattgg ccctgttatt
     6001 gacggctgac ggaggtatgg tatgatcctt tccgtgacgc gacaggataa ctgtttcagt
     6061 gttgcctatt tttccgcata caagagggga agccgggctg ccgtatttat tgctgatatt
     6121 gattatttcg ggtgaatcca gtatttcagg atcttccagc cctgaacctc ctataatgcc
     6181 tatttttacc attattcctt ccccaggtat cgcatgagaa tattaactgc ctcatccatg
     6241 tcctggccga atgtaagtat tcctgcacga tcacctgcca tgacaattat tcttttttca
     6301 aatacatccg aatcgctgaa gagcctgaaa atatcctttg ccagtcctgt tgtcccgtat
     6361 tccatagccg gattggtcgt cggcacctta tatatgattt cgttccacag tgctatactg
     6421 tgaacgtgga ctatggcatt gacgtcggga tcggcaaggt aaattgccgc atgggtaagt
     6481 gattccgatg aggccttgag aggaccggcg cactgtactg cattatcatc aatattgaat
     6541 gaaattactt tcacgtaatg acccggttca agatcgggaa tttctcccgt tgccgagcct
     6601 gttatgacaa actggtttcc gcccctgatc ctcatgctca tgtttccgaa tcccacaccg
     6661 ttttcatagg ctccaatcag atccatatta tacagtattt ccctccagta attgattatt
     6721 tcatactgat catctgatat tacgggtccg cactgggtcc agtggcaatt gaattttaca
     6781 acaccttcca tttgtttatt tgattttatc tgagaacata tttctgattt ccttttctga
     6841 tggcctgcag atgccccttt cggttatcag tcccgcaata agtcttgcag gagtaatatc
     6901 aaaaccataa ttggcaccgc ggacattatc cggacatatt tttatttttt ctattgtttc
     6961 accgttaaat ccctcaataa aaaccagctc ttcctcatca cgttcttcaa caggtatttc
     7021 cttcagtccg tcattgagcc tgaagtcaag ggtagttgac gggaatgctg aatagaaggg
     7081 gatattattg tcatatgcag ccagtgcctt caggtatgtc cctattttgt tggccacatc
     7141 cccctgtatt gttgtacggt cacttccgac aataaccagg tcgaccatcc cgtgctgcat
     7201 cagatgtcct cccgtattgt cagctatcag ggtacaggga acacccgcct ggtccagttc
     7261 ccaggctgtc agcctcgatc cctggttgcg gggcctggtt tcgtctaccc atacgtgaac
     7321 cggaataccg gattcatgag ccaggtaaac cggtgccagg gcggtgccgt aatcaattga
     7381 tgccagccag cccgcattgc agtgagtgag gatattgacg ggattcccgt ttttcctttt
     7441 gcctgcatcc tcaataaggg gcaggccgtt tatcccgata agcctgcaat tctccttctc
     7501 ctgctcacag aggtctagtg ctgccttcag tgcagtttcc cggagtgatg aatcagcagg
     7561 attatttctt atcctgtcca ttacaaaatt cagggcagcg gaaagattca ctgccgtcgg
     7621 gcggcaggaa acaagatatc tggctgcatt ttcaagttga tgaacgtggt cagcattgcg
     7681 cgacaactca aatgtggcca gataaatgcc gaaagctgcc gcggctccga taagaggtgc
     7741 accgcggacc gtcattgtgc tgatggcggt gtatacatct tccacagacc tgagttccct
     7801 tgtctcaaag aaaaagggaa gacggttctg atctatcacc cttacgacag aaggatcaga
     7861 ttcatcaagc cagataccct gataattttt agtgcctgcg agcattatga ttaaataatg
     7921 aaaacaagtc gcgttcagaa accaaattta taaaaataaa aaagatattc gaaaatgatt
     7981 ttgaccgatt tacttgagct ttccaccgga tatcccaata aaagcggatt caaaacttcg
     8041 accggattgg tcgaaatatt tgaatgttga agttttatat tgtaatttta cagaaagaac
     8101 cggtcagtct ccagtcaaga gttatatggt taataaagag gaatatagaa agcggatcat
     8161 taagatctcc ggcagcattt tcagcaggta cggatttaag aaaacctcga tggatgagat
     8221 tgccagggct cttaagatgg gtaagagttc catttattac tatttcaacg gtaaggaaga
     8281 aatattcaag gcagtggtga tgaatgaggc caatattctt agaaatgagc tgactaagac
     8341 tatcaaggca gttgattcac ctactgaaaa actgcggaac tatgtttttg tcaggatgaa
     8401 atcgttcgaa aaactatcaa attattacaa tgccatcttt gacaagaacc ttgaccattt
     8461 tgatttcata gagagtgtca gaaccaaata cgaccgtgaa gagcttgcaa ttctgagact
     8521 tattctctat gatggtgcca ggaagaaggt cttcaatgta cccaacagtg aatatactgc
     8581 ccttgctgtc cagaccgccc tcaagggtct ggaagtgcct cttttctgga aaaggaagga
     8641 gatagacatt gaaaacaggc tgaatggcat cctggatata ctgttccacg gaatacttgc
     8701 aaaatagcag cttcctcccc ggacactgtt ttgacactga tccgggcaca ttctgtatta
     8761 ttttcctttt ccctgcaggg atgacgtaaa agcagttcag ccctttcttt ttattgtggc
     8821 ggtctttttg tttttcaggc tgtgccagag ccagatacag ccggcaacga caaagggaat
     8881 gctgagtaac tgacccatgt tcaatgccat tccctgttca aaatcaacct ggtcttcctt
     8941 caggaattca agaaagaacc ttgcagtgaa aatcagtatc atagccagac tgataagcgt
     9001 gccctggtag tgttctccct ttttcctcca gtacaggcgg taaaggagaa tgaatatcat
     9061 aaggtatgcg agggcttcat agatctgggt tggatgcttc ggggcattct ctccgtttct
     9121 caggaaaacg aaaccccagg gaagagtggt ttccacaccg tatatttcgg aattcatcag
     9181 gttgcctatc ctgataaaaa agcctgcaag tgcaacaaca atggcaatac ggtcaagagc
     9241 ccaggcataa tccttccttt ccttccttac aaacagccag atcgcgatca gtatgccaat
     9301 ggcagcacca tggcttgcca gtcccccgtg ccatatcttc agtatttcac cgggatggga
     9361 gagataataa cccggttcgt agaacaggca gtgcccgagc cttgccccgg caataacacc
     9421 gactgccata tatattgtca gtctgtcaag ggaggcttcc ggaagatttt catttctgaa
     9481 aatcctgttc ataattatat atccgaataa aaaaccggag gcaaacagca atccgtacca
     9541 tcttattgca aaagatccta ccctgaaaat ctcaggatta acatcccatg ggataacaag
     9601 aaaatgcatt tttcagatat tagttaaaag ccaaaggtat aaattatgca aataaagaaa
     9661 accctaatca ggataaagtt ggaaatccgg gaacaaaaga gaagagaaac agctgttccg
     9721 ggtgatacgt atgttgtcgg ccaggaccgg accggataag attaccggat ggaataagaa
     9781 acctgctgct gattaaagtt actgagctga ttacattgat aaaaaaattg atgtaagttt
     9841 gtaaaccctt aaaaaacccg gatcggatgg agaaaacagg catattaagg tatatcgcgt
     9901 atgttgtcag cggatttatg ctggcagccg aatgtgccaa gatcagctct ccatcgggcg
     9961 gaccccaaga tacagagccc
//
LOCUS       DAOSPX010000086         9946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6582_length_9946_cov_5.322819, whole genome shotgun sequence.
ACCESSION   DAOSPX010000086 DAOSPX010000000
VERSION     DAOSPX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6582_length_9946_cov_5.322819"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PLZ75_05890"
     CDS             complement(<1..1123)
                     /locus_tag="PLZ75_05890"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOS71964.1"
                     /translation="MAQEDIFKKLTAHCKEYGYVFPSSEIYDGLSAIYDYGQYGVELK
                     NNIRKYWWDSMVLLHENIVGIDSAIFMHPTIWKASGHVDAFNDPLIDNRDSKKRYRAD
                     ILIEEHMAKIGEKIDREIEKAAARFGEAFDEKKFRETNPRVLAYQEKIDGIFARFSKA
                     MNDNDLQELRQIIVDNEIVCPVSGTRNWTEVRQFNLMFSTEMGSTAEGSSKVYLRPET
                     AQGIFVNFLNVQKTGRMKLPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRPG
                     TELEWFEYWKEARMKWFRALGFGDDNYRFHDHTKLAHYANAATDIEYKFPFGFKEVEG
                     IHSRTDFDLRQHQQYSGKKMQYFDPEKGESYIPYVVETSI"
     gene            1237..2814
                     /gene="nadB"
                     /locus_tag="PLZ75_05895"
     CDS             1237..2814
                     /gene="nadB"
                     /locus_tag="PLZ75_05895"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOS71965.1"
                     /translation="MKRKTDFLVLGSGIAGLTFALKACRYGKVTIVTKRRMEDSNTRY
                     AQGGIASVFREPDNFEKHISDTLIAGAGICDEEVVRMVVSEAPERIKDLIELGVPFDR
                     KEDGSYDLAREGGHSEYRILHYKDKTGEVIGETLIERVKREPNIEIFENHFAIEVLTQ
                     HHLGEVVRRSHPGIKCYGAYVADLVNQKVITFLSRITVMATGGIGNVYLTTTNPEIAT
                     GDGIAMVYRAKGTIENMEFVQFHPTSLFDPNTRPSFLITEALRGYGAVLKNLKGEDFM
                     SRYDSRGSLAPRDIVVRAIDTEMKIWGDDHVWLDCTHLDPEGLKDHFPTIYDHCLTRG
                     IDITRDMIPVVPAAHYSCGGVKVDKNGQSNIDRLYAIGEVASTGLHGANRLASNSLIE
                     AAVYAHRAALHSGARLAGMGFEENIPDWDYKGTAHLEEMVLITQSLKELQGIMSNYVG
                     IVRSDLRLGRALVRLEILYEETENLYKSSLISQKICELRNLINVGYIIIKFARYRKES
                     VGLHYSIDYPKRGISEF"
     gene            2827..3765
                     /locus_tag="PLZ75_05900"
     CDS             2827..3765
                     /locus_tag="PLZ75_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOS71966.1"
                     /translation="MNHLESSFAGKNAFWRYLIMIAAVIAVANTIGALPLIISILRNP
                     AAAGELASSPGDFTVLGISSITAFAFLLIPFIIALLAFILLIRPLNGRSFMAVINGTG
                     TFRWNRFFLSAAVWISISVIYLLVSLKLDPANFSVNNLSSSLILLVIVSLLLVPFQAA
                     FEEVLFRGYLMQGFTVLFRNRIFPLVVTSILFGLMHSLNPEVGEFGFLTMMPQYILFG
                     LIFGIVAVMDDGIESAIGIHAANNVFLCIMVTNKSSALQTPAVWEQHTIQPWTEFVFM
                     FIMGALTIFILRKIFRWGSFSLLFGKVEKPGNTENP"
     gene            complement(3837..4850)
                     /gene="dusB"
                     /locus_tag="PLZ75_05905"
     CDS             complement(3837..4850)
                     /gene="dusB"
                     /locus_tag="PLZ75_05905"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOS71967.1"
                     /translation="MTIGNISLGDYPLFLAPMEDITDPSFRYVCKLSGADFMYTEFIS
                     SDGLIRNGQKSVDKLDIRDFDRPIGIQLYGHITGAMVEAAHMAEAAKPELIDINFGCP
                     VRKIAARGAGAGMLKNIPLMIEMTSAIVRAVNLPVTVKTRIGWDDENKNIMEGAERLQ
                     DAGIAAITIHGRTKAQLYRGKADWTLIGEVRKNPGIKIPVIGNGDIDGPLRAREMFSR
                     YGVDGIMIGRAAVGRPWIFRDIKHFLSTGELLPEPTTEEKADLALLHFEKSLEFKQGK
                     RAILEMRRHLSNYFKGLPHFRETRLKLLTSTEPDEIRAIIRETGNKYGSFRTEDKTSV
                     YQD"
     gene            complement(4884..5627)
                     /locus_tag="PLZ75_05910"
     CDS             complement(4884..5627)
                     /locus_tag="PLZ75_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71968.1"
                     /translation="MPYRRLPNTDAARMRAMKKALKTSRELPPNRMAISPRSLVALQR
                     FVPLFEQEILLQRQAFIFQQNRSGDYSEISRKARLYLTHFMRVMNMAIQRGELPPETR
                     EYYGLKKNDSSLPSLRTENELITRGQRIIEGEEQRIRDGGCPITNPTIAVVKVRFRSF
                     LEFNSHNKSVAKRVRDYSFKAKGFREEADELILQIWNEIEGYFAHLPREIRLKECENH
                     GVVYFYRKSEKIKQAISGDQLSVYYKKVG"
     gene            5808..7199
                     /locus_tag="PLZ75_05915"
     CDS             5808..7199
                     /locus_tag="PLZ75_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOS71969.1"
                     /translation="MFSKEVYSRRRSELRGRMEGGIGLFPGNTEAPMNYPANPYRFRQ
                     DSDFLYFFGLDLPGFAGLIDFDSGKEILFGNDFDMDDIVWMGPQPTVRELALRSGITE
                     TAPLAELETFIAGALSKNRKIHFLPPYRGDTKIMLASLLKENPCSMKTAPSADLVKAV
                     VSLRSLKGEAEISEIEKAVDIAREMHVTAMKMCRPGVKEQEIAGAIEGIALSMGAGVS
                     FPVILSMDGQTLHNHSHDNILEKGRMMVTDAGAETNLHYASDITRTTPVGGKFSTLQK
                     EIYETVLKANIEAINATRPGVSNRDVHLLACRVLAGGLKDLGLMKGDVDEAVALGAHA
                     LFMPHGIGHMLGLDVHDMEALGENFVGYSDDLRRSEQFGLAFLRFALPYVPGHVFTIE
                     PGCYFIPELIESWKAEGKFTDFISYGQLEKYMSIGGIRIEDNILITAGGHKVLGRPIP
                     KTVREIEELCSRD"
     gene            complement(7228..7962)
                     /locus_tag="PLZ75_05920"
     CDS             complement(7228..7962)
                     /locus_tag="PLZ75_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOS71970.1"
                     /translation="MNLTLKIWRQKNAKAKGNFVTYKMENVSPAMSFLEMLDALNEKL
                     VRENEDPVAFDHDCREGICGSCGMYINGHPHGPVPAVTTCHLSMRYFSDGDTIRIEPW
                     RARAFPVIKDLIVDRSAFDRIQSEGGFISINTGSAPEANLIPVRKADADTAFDASICI
                     GCGACVAACKNASAMLFVSARVSQYALLPQGRTEARERVRAMVSIMDELGFGSCSNTG
                     ACEAECPKEIKLTNIARLNREYHKAY"
     gene            complement(8140..>9946)
                     /locus_tag="PLZ75_05925"
     CDS             complement(8140..>9946)
                     /locus_tag="PLZ75_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOS71971.1"
                     /translation="VVGTGLSGASAASALGELGYNVSNFCYQDSPRRAHSVAAQGGIN
                     AAKNYRNDGDSVYRLFTDMIKGGDFRSREANVYRAAEVSNQVIDHFTAIGVPFARDYG
                     GTLDTRSFGGVQVSRTFYVKGQTGQQCLLGCYSSLNRQIKKGTVKMFPRREMLDLVLI
                     GGKARGIITRNLVTGEIERHGAHAVVLATGGYGTIYYLSTLAVNSNASAAWKAYKKGA
                     FFANPAFIQIHPTCVPQMNEFQKKLTLMSESLRNDGRVWVPLRADDERRPNDIPEGER
                     DYFLERRYPAFGNLVPRDVASRAAKERCDAGFGVGETKLAVYLDFRDAIEKQGKKVIE
                     EKYGNLFQMYKTITGSDPYEEPMRIYPAGHYTMGGLWVDYELMTTIPGLYAIGESNFS
                     DHGANRLGASSLMQAAGDGYFILPNTINNYLAGEIQQPKISTDAPEFEEAERSVTGRI
                     NRLMAVRGTQSASAFHRRLGKIMWDHVGMVRNGKGLEEALGMVAELEDEFWKDLLIPG
                     SPDELNHELEKAGRVADFFELARLMITDALHRRESCGAHFREEYQTPDGEAMRNDREF
                     AHVAVWEYAGEDREPVLHREKLVFEEAEMKIRSYK"
BASE COUNT         2335 a         2488 c         2409 g         2714 t
ORIGIN      
        1 cgattgaggt ttcaaccacg tagggaatat atgattcacc tttttccgga tcgaagtact
       61 gcattttctt tccgctgtac tgctgatgct gacggaggtc gaaatcggtt ctcgaatgta
      121 ttccctcaac ttccttgaag ccgaaaggga atttatattc tatgtcggtt gctgcattcg
      181 cgtaatgggc aagttttgta tgatcgtgaa agcggtaatt gtcatctccg aatccgagtg
      241 ccctgaacca tttcatcctg gcttccttcc agtattcaaa ccattcaagc tccgttcccg
      301 gcctgacaaa gaactgcatt tccatctgtt caaactcgcg catccggaag atgaactgac
      361 gtgcaactat ttcattgcgg aaggcttttc cgatctgggc aatcccgaag ggcagcttca
      421 tcctgcccgt cttctgcacg ttgaggaaat tgacgaaaat accctgggcg gtttccggac
      481 ggagatatac cttgcttgat ccctcggctg tggagcccat ttcagtggaa aacatcaggt
      541 tgaactgtct tacctctgtc cagttgcggg ttccggaaac gggacagaca atctcattgt
      601 caacaatgat ctggcggagt tcctgcagat cgttgtcatt catggccttt gaaaaccttg
      661 caaatatccc gtctatcttt tcctgatatg caagcacacg cggatttgtt tccctgaatt
      721 tcttttcgtc aaatgcctca ccgaagcgtg cggcggcttt ttcaatctcc ctgtctatct
      781 tctcgccgat ttttgccatg tgttcttcga tcaggatatc ggcgcggtat ctctttttgg
      841 aatccctgtt gtcaatgaga gggtcgttga aagcatccac atgtccggaa gccttccaga
      901 tagtgggatg catgaatatt gccgaatcaa tgcctacaat gttttcatga aggagaacca
      961 tgctgtccca ccagtatttc ctgatattgt ttttcagttc aacgccatac tggccgtaat
     1021 catagattgc actcaatccg tcatatatct cgcttgaagg aaacacatag ccgtattctt
     1081 tgcagtgagc cgtcagcttt ttaaaaatat cttcctgagc catagggttt ttattatgag
     1141 ggaggcaaaa ttaattcaaa aaatctgctt tcacaaaggc taattcatct cattgattct
     1201 attattatct ttatcagttc aaaattcatt gcctgtatga agcggaaaac ggattttctt
     1261 gttctgggat cgggtattgc aggactgacc tttgctctca aggcctgcag atacgggaag
     1321 gtgaccattg taaccaagcg gcggatggaa gattcaaaca caaggtacgc ccagggcggt
     1381 attgcctctg ttttcaggga accggacaat tttgagaagc atatcagcga cactctcatt
     1441 gccggagccg ggatctgtga tgaggaggtg gtgagaatgg tggtcagcga ggcaccggaa
     1501 aggataaagg atctgataga acttggtgtt cccttcgaca ggaaggagga cggatcctat
     1561 gatctcgcaa gggaaggggg gcattccgaa tacaggatac tgcactacaa ggacaagaca
     1621 ggggaggtca tcggagagac actcattgag agggtaaaaa gggagccgaa catagagatc
     1681 tttgaaaacc attttgcaat agaggttctt acacagcacc acctgggaga ggttgtcagg
     1741 cgcagccatc ccggaataaa atgctacgga gcctatgttg ccgatcttgt aaaccagaag
     1801 gtgatcacct ttctttcgcg catcacggtc atggccacgg gcggaatagg aaacgtgtac
     1861 ctgaccacca ccaatcccga gatagccacg ggcgatggca tagcaatggt ataccgggcc
     1921 aaggggacta tcgagaatat ggaattcgtg cagtttcatc cgacatccct ctttgatccc
     1981 aataccaggc cgtcatttct tatcacagag gcactgaggg gatacggggc agtgctgaag
     2041 aacctgaaag gtgaagactt catgagcagg tatgacagcc gcggctccct tgccccgcgg
     2101 gacattgttg tgcgggccat tgacactgaa atgaagatat ggggggatga tcatgtatgg
     2161 cttgattgca cacaccttga ccctgaaggg ctgaaggatc acttcccgac aatatatgat
     2221 cattgcctca ccaggggaat tgatatcacc agggacatga tccccgtggt tccggcagct
     2281 cattattcct gtggcggtgt taaggttgac aagaacggac agagcaacat agacaggctt
     2341 tatgccatag gcgaagtggc atcaaccggt ctgcatggtg caaacagact ggcttccaac
     2401 tcactgattg aagcagcagt atatgctcac cgggctgccc ttcactcggg agcaaggctt
     2461 gccggaatgg gtttcgagga aaatatccct gactgggatt acaagggaac cgcccatctc
     2521 gaggaaatgg ttctgataac acagagcctg aaggaattac agggaatcat gagcaattat
     2581 gtagggatag ttagatcgga tcttcgtctg ggaagggcac tggtccgcct ggagatactc
     2641 tatgaagaaa ccgaaaacct ttataaaagc tcgcttatat cgcagaaaat ctgtgaactg
     2701 cgtaacctga tcaatgtggg atatataata ataaaatttg ccaggtacag gaaagagagt
     2761 gtagggcttc attattccat tgattacccc aaaagaggca ttagtgaatt ctaaataaat
     2821 cagtcaatga atcatcttga atcatcattt gccggcaaaa acgccttctg gagatatctt
     2881 atcatgattg ccgcggttat tgccgtagca aataccatag gggcgctgcc gctgattatc
     2941 agcatactcc ggaatcccgc agcagctgga gaactggctt ccagtccggg cgattttact
     3001 gttctgggaa tcagttcaat aactgccttc gcctttttgc tcatcccctt cattattgca
     3061 ttgcttgctt tcattctcct catccgtccg ctgaacggca gaagctttat ggccgtgata
     3121 aacggaactg ggacattcag gtggaacagg ttcttcctgt cggcagcggt atggatcagt
     3181 atctcggtta tttacctgct ggtgagcctg aaactcgatc ctgccaattt ttcggtcaac
     3241 aacctctctt catcgctcat cctgctggtg attgtttcat tattgctggt gcccttccag
     3301 gctgccttcg aggaggttct gttcaggggc tatctgatgc agggtttcac ggtgcttttc
     3361 cggaacagga tctttcccct tgttgttact tccatcctgt tcggactgat gcattccctc
     3421 aatccggaag tcggtgaatt cggcttcctc acaatgatgc cccagtacat actgttcgga
     3481 ctgatattcg ggattgttgc agtgatggat gacgggattg aatcagccat cggaattcat
     3541 gcagccaaca atgttttcct ctgcataatg gtcaccaata aatcatcggc cctgcagact
     3601 ccggccgtct gggagcagca taccattcag ccctggacgg aatttgtgtt catgttcata
     3661 atgggggcac tgacaatttt tattctccgg aagatattca gatggggcag cttcagcctg
     3721 cttttcggaa aggtggaaaa accggggaat accgaaaacc cctgaaacaa gcttcacggc
     3781 tcagccgggg tcaaatgatg tctgtcgtca ggaccggagg agcagatgtt ttagcgtcag
     3841 tcctggtaaa cagatgtttt gtcttcggtc cggaagctac cgtatttatt cccggtttcc
     3901 cttattattg ctcttatctc gtcgggttca gtgcttgtca gcagcttcag gcgggtttcc
     3961 ctgaaatggg gcagtccctt gaaataattt gaaagatgtc ttcgcatttc aagtattgcc
     4021 ctttttccct gcttgaactc aagagacttt tcgaagtgca ggagagccag atcagctttt
     4081 tcttccgttg tcggttccgg cagcagctca ccggtactca ggaaatgttt tatatcccgg
     4141 aagatccagg gcctgcccac tgcggcacgt cctatcatta ttccgtcaac cccgtagcgt
     4201 gagaacatct ccctggccct gagcggtccg tcaatatccc cgttgccgat gaccgggatt
     4261 tttatccccg gatttttcct gacctcaccg atcagggtcc agtcggcctt tcccctgtag
     4321 agctgggcct ttgtcctgcc gtggattgtg attgccgcta ttccggcatc ctgaagcctt
     4381 tcggcccctt ccattatatt tttattctca tcatcccagc ctatccttgt ctttaccgta
     4441 acgggaagat ttaccgccct gactattgca gaggtcatct caatcatcag cggaatgttt
     4501 ttcagcatgc ctgctcctgc tccgcgtgcg gcaatcttcc tgaccggaca gccgaaattg
     4561 atgtctatca gttccggttt tgccgcttcg gccatatgtg ccgcttcaac catggctccg
     4621 gtaatatgtc cgtaaagctg aatccctatc gggcggtcaa aatcccggat atcgagcttg
     4681 tccacgctct tctgtccgtt tctgataaga ccgtcggagg aaatgaattc cgtatacatg
     4741 aaatcggcgc cggacaattt gcatacatag cggaatgaag gatcggtgat atcctccatg
     4801 ggagcaagaa agagcgggta atcacccagt gatatattgc cgatggtcaa gacagataaa
     4861 tttttgatat tgaatgagtc cgctcagcca acctttttat agtaaacgga aagctgatct
     4921 ccggaaatgg cctgttttat cttttcactt tttctgtaaa aataaacaac tccatggttt
     4981 tcgcattcct tcaggcggat ttcacgcgga agatgtgcaa aatacccttc tatctcattc
     5041 catatctgca gtatgagctc atcggcttcc tccctgaagc ctttggcctt aaaagaatag
     5101 tcgcgtacac gttttgcgac ggatttgttg tggctattga attccagaaa gctccggaac
     5161 cttactttca ccacggcaat tgtcgggttt gtaataggac agccgccgtc tcttattctt
     5221 tgttcttcac cctcaataat tctctgtccc cgggtaataa gttcattctc tgttctcagg
     5281 gaaggaaggg aactgtcatt ttttttcagg ccgtagtatt ccctcgtttc cggcggcagt
     5341 tccccgcgct ggattgccat gttcatcacc cgcatgaaat gggtgagata gagcctggcc
     5401 tttcttgata tctcactgta gtccccgctg cggttctgct gaaagataaa agcctgtctc
     5461 tgaagcagta tctcctgttc aaacaagggc acgaacctct gaagggcaac cagcgacctg
     5521 ggcgagattg ccatcctgtt gggaggcaat tcccgtgatg ttttcagtgc ttttttcatt
     5581 gccctcatcc gggcagcatc cgtgtttggc aatctgcggt atggcatgaa actgaatttt
     5641 atgtaccttt ctggaaactt atacgaatat agtgtcattt tgatgcactt tccggctctt
     5701 cgctgttcat aacagtgaat ttatcaacag ctgtttgcct gcgggtgatt gtttcatgaa
     5761 tcaatgatat taacttagcg cattattaac aaacaggaag aattcttatg ttcagcaagg
     5821 aagtatattc aagaagaagg tcggagttac gcggcaggat ggaaggcgga atcggattat
     5881 tccccggaaa cactgaggct ccgatgaatt atcctgccaa tccctaccgg ttcaggcagg
     5941 acagtgattt cctttatttt ttcggtcttg atctgccggg atttgcaggc ctgatagatt
     6001 ttgattccgg aaaggaaatc cttttcggaa atgatttcga catggatgat atcgtatgga
     6061 tggggcccca gcccactgtc agggaactgg cactccggag cggtataact gaaacggctc
     6121 ccctggcgga gcttgagact ttcatcgccg gagccctgtc gaaaaacagg aaaatccatt
     6181 ttctgccgcc ctaccgcgga gatacaaaaa tcatgcttgc ttccctgctg aaagaaaatc
     6241 cctgcagcat gaaaaccgct ccctcagctg atcttgtaaa ggcggttgtg tcgctgagat
     6301 cactgaaggg tgaagcggaa atcagcgaaa ttgaaaaagc tgttgacata gcccgggaaa
     6361 tgcatgtcac ggcaatgaaa atgtgccggc cgggagtaaa ggaacaggaa attgccgggg
     6421 cgattgaagg tatcgccctc tcgatggggg caggagtctc tttcccggtc attctgagca
     6481 tggacgggca gaccctgcat aatcattcac atgacaatat ccttgagaaa ggaagaatga
     6541 tggtgaccga tgccggagca gaaacaaacc ttcactatgc atcggatata acgagaacca
     6601 ctcctgtggg cgggaagttc agcacccttc agaaagagat ctacgaaaca gttctgaaag
     6661 cgaatattga ggcgataaat gcaacccgtc cgggtgtttc gaaccgcgat gttcatctcc
     6721 tggcatgcag ggtactggcc ggcggactga aggatctggg tctgatgaag ggggatgttg
     6781 atgaagccgt tgcccttggg gcacacgcgc tgttcatgcc tcacgggata ggccatatgc
     6841 tcggactgga tgtccatgac atggaggccc tgggagagaa ttttgtggga tacagtgatg
     6901 acctaaggcg aagtgaacag ttcgggcttg cattcctcag gtttgcgctt ccctatgtac
     6961 ccggacatgt ttttaccatt gaaccagggt gttatttcat tcccgagctc attgaaagct
     7021 ggaaggcgga ggggaagttc acggatttca tcagctacgg ccagctggaa aaatacatgt
     7081 caatcggcgg aataagaata gaggacaata tcctgatcac tgccggaggg cataaggttc
     7141 tgggccggcc cattcccaag accgtaaggg agatagagga actttgttcc cgggattaaa
     7201 ggaaacctca ttgtattgtt tcagggatca gtaagcctta tggtattccc tgttaagccg
     7261 ggctatgttt gtgagtttaa tctccttggg acattcggct tcgcaggcgc ccgtgttgga
     7321 acagcttccg aatccgagct catccattat gctgaccatt gcccttacac gttccctggc
     7381 ttcggtacgt ccctgtggaa gcagtgcata ctgtgatacc cttgccgaca cgaacaacat
     7441 tgccgatgca ttcttgcagg cggcaacaca tgcaccgcag ccaatgcata ttgacgcatc
     7501 aaaagcagta tcggcgtcag ctttccttac agggattaag ttggcttccg gtgcagaacc
     7561 ggtgtttatt gagatgaaac cgccttctga ctgaatcctg tcaaaggcac ttcggtcgac
     7621 gatgagatct tttataaccg gaaaggccct tgccctccag ggctcaatcc ggattgtgtc
     7681 gccatcgctg aaatatctca tcgagagatg gcatgtggta acggcgggaa ccggaccgtg
     7741 gggatgaccg tttatataca ttccgcagga tccgcagatt ccttcccggc agtcgtggtc
     7801 aaaagcaacg ggatcttcgt tttccctcac cagcttttcg ttgagcgcat caagcatttc
     7861 gaggaatgac atcgccggag acacattctc catcttatat gtaacaaagt tcccctttgc
     7921 tttcgcattc ttctgcctcc atatcttcag agtgagattc ataccttccc gatatatgtt
     7981 taattttgtt acttttttca ggcccttttc cggttttccg gctgctcact ttttcgttcc
     8041 ggcctttttt ttgttcattt tcttttttcc cgccttcctt ctgtttccct aatcctgccg
     8101 gcctttctta cgggaggctg attcttcagt tcagctccac tatttataac ttcttatctt
     8161 catttctgct tcctcaaaaa ccagtttttc cctgtgaagc accggttccc tgtcttctcc
     8221 ggcatactcc catacggcaa catgggcaaa ctccctgtcg ttacgcattg cttccccgtc
     8281 gggagtctgg tattcctccc tgaaatgcgc accgcacgat tccctgcggt gcagcgcatc
     8341 ggtaatcatc aggcgggcaa gctcaaagaa atcggcaacc cgtccggcct tctccagttc
     8401 atggttgagc tcatcagggg atcccggaat taaaagatct ttccagaatt catcttccag
     8461 ttccgcaacc attccaagag cttcctcaag tcctttcccg tttctgacca tgcccacatg
     8521 gtcccacatt atcttgccga gcctcctgtg aaacgcagaa gccgattgtg ttcccctcac
     8581 agccatcagt ctgttgatcc ttccggtaac ggatctttcc gcctcctcaa attccggagc
     8641 atcggttgag atcttcggct gctgtatctc accggcaagg taattgttta tagtattcgg
     8701 aagaatgaaa taaccatctc ctgctgcctg catcagtgag cttgcaccga gccggttggc
     8761 tccgtggtcc gagaagtttg actctcctat ggcatagagt ccgggaatgg ttgtcatgag
     8821 ctcatagtca acccagaggc cgcccatggt atagtgccct gccggataga ttctcatagg
     8881 ctcctcatag ggatcacttc ccgtgatggt tttatacatc tggaaaagat tgccgtattt
     8941 ctcttcaatg acttttttcc cttgtttctc aattgcatcc ctgaaatcga gatacactgc
     9001 aagttttgtt tcccccacgc cgaaaccggc atcgcagcgt tccttggctg cccttgatgc
     9061 cacatcacgc gggacgagat tgccgaatgc gggatatctc ctttcaagga aataatccct
     9121 ttccccttcc ggaatgtcat ttggccggcg ttcatcatcc gccctgagag gcacccatac
     9181 cctcccgtcg tttcgaagcg attcggacat cagcgtgagt ttcttctgaa actcgttcat
     9241 ctggggaacg caggtcggat gtatctgtat aaaagccgga ttggcaaaga aggctccctt
     9301 tttgtatgcc ttccatgcag ctgatgcatt tgaattaacg gcaagagtgg aaaggtagta
     9361 gattgttccg tagcctcccg ttgccaggac gacggcatgg gccccgtgcc tttctatttc
     9421 gcctgttacc aggttccggg ttattatgcc tctggctttg cctccgataa gcaccaggtc
     9481 gagcatctcc ctgcggggaa acatctttac ggttcctttt ttgatctgcc tgtttagtga
     9541 agagtagcag ccgagaaggc attgctgtcc tgtctgcccc tttacataga aagtccttga
     9601 aacctgaaca cccccgaagg agcgggtatc gagggtaccg ccgtagtcac gggcaaatgg
     9661 gacaccaatg gccgtgaaat ggtctattac ctggttgctc acctcggccg ccctgtaaac
     9721 atttgcttcc cgtgaacgga aatcccctcc ttttatcatg tcggtaaaca ggcggtaaac
     9781 cgaatccccg tcgttccggt aattctttgc agcattgatt cctccctggg ccgcaacgga
     9841 atgagcccgc ctcggggaat cctggtagca gaaattactt acattgtaac ccagctctcc
     9901 cagtgcagat gcagcggaag ctcccgacag gcccgtgccc acaacg
//
LOCUS       DAOSPX010000087         9900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6610_length_9900_cov_4.376232, whole genome shotgun sequence.
ACCESSION   DAOSPX010000087 DAOSPX010000000
VERSION     DAOSPX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6610_length_9900_cov_4.376232"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..761
                     /gene="pssA"
                     /locus_tag="PLZ75_05930"
     CDS             36..761
                     /gene="pssA"
                     /locus_tag="PLZ75_05930"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOS71972.1"
                     /translation="MKHIPNLITSLNLASGFISVIFVINGDLVTASWLLLAAMIFDFL
                     DGFSARLMKAYSDIGKELDSLADVISFGLAPATIIYRLLERSMELSGPFTAGNTGLTT
                     CAILLSTAIMPVCAGLRLAIFNTDETQSRSFKGLPTPANALAVISLVISARYSGSAAA
                     TWLITSTPALVVLTIILSLLMVSRIPLLSLKTNNLRFRGNEGLYILVLLAGISFVLGG
                     GLGAAYLLIPVYIVSSLAGRLFR"
     gene            complement(844..1971)
                     /gene="cydB"
                     /locus_tag="PLZ75_05935"
     CDS             complement(844..1971)
                     /gene="cydB"
                     /locus_tag="PLZ75_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOS71973.1"
                     /translation="MLTLVSHSFLQGYWWVIISLLGALLVFLMFVQGGQSMIFSISRD
                     EMHKTMVVNALGHKWEFTFTTLVTFGGAFFASFPLFYATSFGGAYWLWIAILFSFTIQ
                     AIAYEYRSKPANVLGKKTFDVFLLVNGIFGPFLIGTAVATFFTGSQFSLDHMNHVRWA
                     NNWHGLEALTDARNLALGLDELYLVRSNGCMYIINSVKDEDLAAKAGRKLMVNAVVFL
                     VFFLFFLVSVLLSEGYAVDGDGKVTLEKYKYFQNFVQMPLVLVLFLAGVIGVLSGIIM
                     TVFRRSRRGIWYSGPGTILAVFSLFLIAGFSGTAFYPSSFDPGSSLTIRNASSSRFTL
                     QTMMYVSFIIPFVFWYIWYAWKSISRKKISEEELSEEPHLY"
     gene            complement(1984..3540)
                     /locus_tag="PLZ75_05940"
     CDS             complement(1984..3540)
                     /locus_tag="PLZ75_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOS71974.1"
                     /translation="MIETADLSLVDWSRAQFALTAMYHWIFVPLTLGLSFIVAMMETV
                     YYRTGKEEWKRMTRFWMTLFGINFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLA
                     IEGILAFFLESTFVAVMFFGWNKVSRKFHLVSTWLVAIGASLSALWILVANAWMQNPV
                     GMTFNPETARNEMTDFLAVLTNPVAIDTFLHTVTSGFLLASVFVLGISAWFLLKGREE
                     WMAGKSILIAGIFGLASSLIVALTGDISARTLAHVQPLKFAAFEAHYSGKDNAGLVAA
                     GILKDSGQLTGDKAKKDFAVKVEIPGFLSVMTGGNRHVYVPGIEDLVYGNPDKGIMPV
                     AEKMERGRYARDILMDYKRARKSNNAGEAEVLRGMFSDKNFIDDYFRYFGYAFLDKPE
                     DVIPNVSVAFYTFHLMVMLGFFFILLCALALFFHYRGSLQKQKWLLWMMMISVPLAYI
                     ASQSGWVLKEMGRQPWIIQDLMPVGTAVSNINSGPVIVTFVLFAILFTVLLIAEVSIM
                     TKQIKTGPKN"
     gene            complement(3533..3784)
                     /locus_tag="PLZ75_05945"
     CDS             complement(3533..3784)
                     /locus_tag="PLZ75_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOS71975.1"
                     /translation="MCSENVSGRGPIGKIFRFYYDGFKNMSSWGRKVWLIILIKLFII
                     FAVLRLFFFPDFLRKNFKSEKERSEYVLEQLTPSHKSYD"
     gene            complement(3811..5094)
                     /locus_tag="PLZ75_05950"
     CDS             complement(3811..5094)
                     /locus_tag="PLZ75_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOS71976.1"
                     /translation="MKPLFRNILIFISVAVLLAGAWYFRSIVVYILVSGVLSIMGRPL
                     VDLFRRIRIGKWSFPGYLAALLALLIIWGLIILFFIIFIPLITRQFNYFSTIDSQKIV
                     MVLERPLNYIQDILRTLNKNINSDFSIQGVINERLSQILDIDRLKSMLGSVFGILGNI
                     AIAIFSITFITYFFLKDQHLFFEAILMWVPDKHTDGFTRALYSIKNLLTRYFIGIVIQ
                     CTCVMILVDIGMTIAGIEFHQALVIGLVVGILNVIPYIGPWLGFFFALVMGVASHVNQ
                     DFATVVIPLIYYMSIVIGMTQLINNVIFQPLIFSNSVRAHPLEVFIVVLAAGFAAGIP
                     GMILGIPAYTVMRVFAREFFYSFKPVQKITSSLATENISSAVSLELDEEGPLHKVVVK
                     TVRKKSSKPRQIRFKPRKPREPRKPRQPGEPLQDE"
     gene            5310..5735
                     /locus_tag="PLZ75_05955"
     CDS             5310..5735
                     /locus_tag="PLZ75_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOS71977.1"
                     /translation="MLPTIRRSFSPFFSGLFDDDFFPVVKSRSSSTLPAVNIRESDKS
                     FILELAVPGIDKKDLNIDISDDVLTISHETKTSSEVDQADGYKRKEFSYSSFCRSFYI
                     PENVLTDKIGASYKDGILTVELPKDKEEKQKLTKQVKIS"
     gene            complement(5797..6453)
                     /locus_tag="PLZ75_05960"
     CDS             complement(5797..6453)
                     /locus_tag="PLZ75_05960"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOS71978.1"
                     /translation="MGIHREGYKILISGFAVLVLVNVAFVLLFPGRTVLLSVAAVVTA
                     ALYVFLLFFFRLPKRHLEPDPELVYAPADGKIVVVEEIEEKEYFKDRRLQVSIFMSPL
                     NMHSNRYPVSGKVKYVQYHPGNYLVAWHPKSSELNERSTVVIETESGKEILIRQVAGA
                     VARRIVTYARVLQDVKQGDELGFIKFGSRVDIFLPPGTEIEIPLLRPVKANKSVLARI
                     "
     gene            complement(6494..7336)
                     /locus_tag="PLZ75_05965"
     CDS             complement(6494..7336)
                     /locus_tag="PLZ75_05965"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOS71979.1"
                     /translation="MKEFYRRTLTGAWIVIFILGGFWLHPVTFCLTGLVIMSGILYEY
                     YRIINGSGTRVQTVTGMVTGIAVYLLSILIAAGLLEYRFFLLVIPLIAMIMTAELYRR
                     DEKPFDSLAHTFFGLIYAVVPFSLFPFSAFSRSGIKPIIPCESIDFSPGMVVGFFILL
                     WINDTGAYLTGMTMGKHRLMERISPKKSWEGFIGGTVLTALTASLLSSRLGILDSAGW
                     VVVALIVAISGTYGDLIESMLKRSTGIKDSGSIMPGHGGFMDRFDSTMLAFPLVFLFI
                     SLFG"
     gene            complement(7352..9424)
                     /gene="ftsH"
                     /locus_tag="PLZ75_05970"
     CDS             complement(7352..9424)
                     /gene="ftsH"
                     /locus_tag="PLZ75_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOS71980.1"
                     /translation="MTQNTNKSSPGKPGDKGAKPKFNTNWIFAIIALSFILFQVFFSR
                     NVTEKATTNMIKDMIARHDIEKLVVVNKDQAEIYLTRDALNSGRYENLPKPGGGGMGL
                     QVPKPHYTYNIGDISNFEPFIIETQKSAGYSENELIYPEYVTRKNYFADILGWLLLPA
                     LLIMVWIFMMRRMTGGGAGGAGGIFSVGKSRAQIFDKDTHVKLNFNDVAGLEEAKTEV
                     MEIVDFLKNPKKYTQLGGKIPKGALLIGPPGTGKTLLAKAVAGEANVPFFSISGSDFV
                     EMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAVGRARGKNVNFSANDERENTLNQLL
                     TEMDGFGTNVGVIILAATNRADILDRALLRAGRFDRQIHVELPDLNEREAIFQVHLRP
                     IKLEQNFDVSFLAKQTPGFSGADIANVCNEAALIAARKNKTVVEKQDFLDAIDRIVGG
                     LERKSKIISIEEKRTIAYHEAGHATVSWLLEHASPLLKVTIIPRGRALGAAWYLPEER
                     QITTTEQLLDEMAYSLGGRAAEELVFGKISTGALSDLEKVTKQAYAMVSYFGMSDKVG
                     NISFYDSSGQSDYGFTKPYSEKTAELIDREVKEIIEQSYERAKNVLRENMKGLSKLAE
                     LLLEQEVIFSEDLKKIFGKRKADRIREEKEKENRKKAKASKGTETEEADLNAAGNPEE
                     ITEGPGEK"
     gene            complement(9432..9809)
                     /gene="rsfS"
                     /locus_tag="PLZ75_05975"
     CDS             complement(9432..9809)
                     /gene="rsfS"
                     /locus_tag="PLZ75_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOS71981.1"
                     /translation="MKKWPAGTEVLLQSVAKGIYDKQGKNVLRIDMRGLENRIADFFV
                     ICHGTSVTQVNSICESVEDVVRKEAGEKALHVEGLGNCFWVLIDYGDVIVHIFLEEYR
                     NFYSLESLWADALIEVMEEKVSK"
BASE COUNT         2735 a         2525 c         2052 g         2588 t
ORIGIN      
        1 taattttaac acgattccct atttaaacat tcaggatgaa gcatattccc aatttaataa
       61 catcactcaa tctcgcctcg ggattcattt cagtcatttt tgtgatcaac ggcgacctgg
      121 taacagcttc ctggctcctg ctggcagcaa tgatatttga tttcctcgac ggtttttcgg
      181 caagactgat gaaagcctat tcagacatcg gaaaagaact cgattcgctg gcagatgtaa
      241 tcagtttcgg acttgctccc gcaacaatta tttacaggct tctggaacgc tcaatggaac
      301 tgtccggacc gttcaccgcc ggcaatacag gcctcactac ctgtgccata ctcctctcaa
      361 cggcaattat gccggtttgt gccggactgc gcctggcaat attcaatacc gacgaaacac
      421 agtccagatc cttcaaagga ctgcccactc cggccaatgc cctggcagtg atttcactgg
      481 ttatctccgc ccgctattcc ggttccgcgg cagccacatg gctcatcact tcaacacctg
      541 cactggttgt cctgactata atcctgtcac ttctgatggt aagccgcata cccctccttt
      601 ctctgaaaac caataatctg agattcaggg gaaatgaagg actctatatt ctcgttctgc
      661 ttgcaggcat ttcttttgtc ctcggcggcg gattaggtgc agcatacctg ctgataccgg
      721 tttatatagt atcctccctt gcgggccggc tgttcagata attcagagcg ctggtccgga
      781 aacgactgta ttacctgaag gagttgtctt tacctgccgg aacgccattc aaaagagagc
      841 cggctaatag agatgcggtt cttcactcag ttcctcttca cttatctttt tccggctgat
      901 cgatttccat gcataccaga tataccagaa cacaaagggt ataatgaagg agacgtacat
      961 cattgtctgc agggtgaacc ggcttgatga tgcattcctt attgtaagag agcttcccgg
     1021 gtcaaaggaa gagggataga aggcggttcc gctgaaccct gcgatcagga aaagggaaaa
     1081 tactgccaga atggtacccg ggcccgaata ccagatcccc ctgcggcttc tccggaagac
     1141 agtcattatg atcccgctca gaacaccaat aactcctgca aggaacaata ccagcaccag
     1201 gggcatctgt acgaaattct gaaaatactt gtatttttcc agtgtcactt ttccgtcgcc
     1261 gtcaaccgca taaccttcag acagaagaac ggaaaccagg aaaaacagga agaaaacaag
     1321 aaacacaaca gcattcacca tcagtttcct gccggctttc gctgccagat cctcatcttt
     1381 caccgagtta atgatataca tgcatccgtt tgaccgtacc aggtagagtt cgtcaaggcc
     1441 cagggcaagg ttcctggcgt cggtcagtgc ttcaagtccg tgccagttgt ttgcccatct
     1501 tacatgattc atatggtcga ggctgaactg tgagccggta aagaaggtgg ccaccgcggt
     1561 accgataagg aaagggccga agattccgtt gaccagaaga aaaacatcaa aggttttctt
     1621 tcccaggaca tttgccggct tcgacctgta ttcataggca attgcctgga tggtgaagct
     1681 gaacagtatc gctatccata gccagtaggc acccccgaag ctggttgcat agaaaagcgg
     1741 aaacgaggca aagaatgccc cgccaaaggt gacaagtgtt gtaaaggtaa attcccattt
     1801 atgtccgagt gcattgacaa ccatggtctt atgcatctcg tcacgggaaa ttgaaaagat
     1861 catcgactgg cctccctgta cgaacataag gaagacaagc aatgcaccca gcagggatat
     1921 gataacccac cagtagcctt gcagaaatga atgtgaaaca agtgtaagca ttgtactgtc
     1981 ctcctaattt tttggtccgg tttttatctg ttttgtcatt atggatacct cagcaatcag
     2041 aagcacggta aacagaattg caaaaagaac aaatgtgaca atcaccggtc cgctgttaat
     2101 atttgatacg gcagtgccta ccggcatgag atcctgtatt atccagggct gacggcccat
     2161 ttccttcaga acccagcccg actggctggc tatatatgcc agcgggacgg aaatcatcat
     2221 catccacagc agccattttt gcttctgcag gcttcccctg taatggaaaa agagtgcaag
     2281 ggcacaaagg agaatgaaaa agaatcccag cataaccatc agatggaatg tataaaaggc
     2341 cacagacaca ttcggtatca catcctcggg cttatccaga aaagcatagc cgaaatacct
     2401 gaaatagtca tcaataaaat ttttgtcact gaacattccc ctcagtactt cagcctcacc
     2461 ggcattgttt gacttccttg cccttttata atccatcagt atatccctgg catacctgcc
     2521 tctttccatc ttttcggcaa ccggcataat gcctttatcc ggatttccgt acacaaggtc
     2581 ttcaataccg ggaacatata catgcctgtt tccgccggtc atcacggaaa ggaatccggg
     2641 aatctccacc ttcacggcaa aatccttttt tgccttgtca cccgtaagct gaccggaatc
     2701 cttaaggata ccggcagcca caagacctgc attgtccttt ccggaatagt gcgcctcaaa
     2761 tgcagcaaac ttaagcggct ggacatgtgc gagggttctt gccgatatgt ctcccgtcag
     2821 tgcaacgatc agggaagagg ccaggccaaa tattcccgca ataaggatac ttttccccgc
     2881 catccattct tcacggcctt tcagcagaaa ccaggcactg atgcccaata caaagactga
     2941 tgccagcagg aatccggaag tgacggtatg aaggaaggtg tctatggcaa ccggattggt
     3001 caggactgcc agaaaatcag tcatttcatt ccttgcggtt tcaggattga atgtcatgcc
     3061 aaccggattc tgcatccatg cattggcaac cagtatccag agggccgaaa ggctggcacc
     3121 gattgccaca agccaggtcg aaaccagatg gaacttccgg ctcaccttgt tccatccgaa
     3181 aaacataact gcaacaaagg tcgattcgag aaaaaatgcc agaatgcctt caatagccag
     3241 gggagctccg aaaatatccc cgacgaacca ggaatagttc gaccagtttg tcccgaattc
     3301 aaattcaagt atgatcccgg ttgccacccc tatggcaaag ttaattccaa agagggtcat
     3361 ccagaatctt gtcatcctct tccactcctc ctttcctgtt ctgtaataga cagtttccat
     3421 cattgcaaca atgaatgaca gtcctagagt cagcggaaca aagatccagt gatacatggc
     3481 agtcagggca aactgtgccc tcgaccagtc gacaagtgac aaatccgcag tttcaatcat
     3541 aagatttatg tgagggtgtt aattgttcaa gaacatattc acttctttcc ttttcgcttt
     3601 taaaattttt cctgagaaaa tcaggaaaaa agaaaagcct gaggacggcg aaaataataa
     3661 aaagctttat aagaattatc agccagacct ttctgcccca ggacgacatg tttttaaatc
     3721 cgtcatagta aaaacggaaa attttcccta tcggcccgcg gccggaaaca ttctcagaac
     3781 acatcagacc aattgtatta gttgtatctc ctattcatcc tgcaggggtt cccctggctg
     3841 cctgggctta cggggctcgc ggggcttacg gggcttaaac ctgatctgcc ggggcttgct
     3901 gcttttcttc cggaccgttt tcacgaccac cttatgcagg gggccctcct cgtccaattc
     3961 cagggatacc gcagaagaaa tattttctgt agccagactt gaagtaattt tctgaacggg
     4021 cttgaagctg tagaaaaact ccctggcaaa gactctcatg acagtatagg caggtatgcc
     4081 cagtatcatt cccggtattc ctgcagcaaa gccggcggcc agaaccacaa taaatacctc
     4141 caggggatgg gccctcacgc tgtttgagaa gataagaggc tgaaatatca cattatttat
     4201 cagttgtgtc atgcctatca ctatcgacat ataatatatc agtggaatca ccacggttgc
     4261 aaaatcctgg tttacatgcg atgcaacacc catcaccaga gcaaagaaga atcccagcca
     4321 tgggccgata tagggtatta cattaaggat gccgaccacg agcccgataa ccagcgcctg
     4381 atggaattca atgcccgcaa ttgtcattcc gatatccaca agaatcatta cacaggtgca
     4441 ttgaatcact atgccgatga aatagcgggt gagcagattc ttgatcgaat acagtgccct
     4501 tgtaaatccg tcagtgtgct tgtcaggaac ccacatcagt atagcttcaa agaaaagatg
     4561 ctgatccttg agaaaaaagt aggtgatgaa tgtgattgaa aaaatggcta tggcaatatt
     4621 tccaagaatt ccgaagacag agcccagcat gctcttcagc cggtcaatat ccagaatctg
     4681 ggacaatctt tcatttataa caccctgtat tgaaaagtca ctgttaatat tcttattgag
     4741 tgtcctcagt atgtcctgaa tataattcag gggcctttca agcaccatta ctatcttctg
     4801 gctgtcaatg gtggaaaaat agttgaactg cctggttata agaggtataa atataataaa
     4861 gaagagtatt atcagacccc agatgatcag aagtgcaagc aaggctgcca ggtatccggg
     4921 aaatgaccat ttgcctattc tgatcctgcg gaagaggtca accagcggac gtcccattat
     4981 ggaaagcaca ccggaaacca gaatatatac cactatgctg cggaaatacc atgctcctgc
     5041 aagcaatact gcaacactga tgaatatgag tatattcctg aatagcggct tcattgaatg
     5101 gcttataccc ctgcaaacct aattgaaatt ttccgtttta acaatttgaa ataaaccatc
     5161 agcagaattt tagccattct gccattttga ccgtcaaaca tccagtgttt cagccattat
     5221 gtcatatttt caatttggaa cggaacttga aatacatgga gtgtttgatt ttaaaaaaag
     5281 tttaattaaa aataatggag gataaagcta tgttaccaac aatcagaaga agtttcagcc
     5341 cgtttttctc aggtcttttt gatgatgatt tttttcccgt ggtaaaaagc cgcagcagca
     5401 gcaccttacc cgcagtcaac atcagggaaa gcgacaagag ttttattctt gagcttgccg
     5461 tcccgggcat agacaaaaaa gatctgaaca tcgatatcag cgatgatgtt ctcacgattt
     5521 cccacgaaac caagacatca tccgaagtgg atcaggccga cggctacaag agaaaggaat
     5581 tcagctactc atctttctgc aggagtttct acattcccga gaatgtactc acagacaaga
     5641 taggtgcaag ctacaaggat ggcattctta cagttgaact tccgaaagac aaggaagaaa
     5701 aacagaaact gacaaagcag gtaaaaatct cataagtcgg ttttacagct taggtttaaa
     5761 gaaaagaggc tgaaatcagc ctcttttctt tcgtttctat atgcgggcaa gcacgctctt
     5821 gtttgcctta acaggccgca gaagcggaat ctcgatttca gtccccggtg gcaggaagat
     5881 atccaccctg gaaccaaact tgatgaatcc cagttcatca ccctgcttca catcctgaag
     5941 gaccctggcg taggttacta ttcttcttgc aacagcgcct gccacctgtc tgatcagtat
     6001 ttccttcccg ctttccgttt caataaccac ggtgctccgc tcattcagtt cagaggactt
     6061 gggatgccat gccaccaggt aattacccgg atggtattgt acatatttta cctttcctga
     6121 aaccggatat ctgttgctgt gcatgttaag aggagacatg aagattgaaa cctgaagcct
     6181 cctgtccttg aaatattcct tttcctctat ttcctcaaca accacaatct taccgtcggc
     6241 cggtgcataa accagttccg gatcaggttc cagatgcctt ttcggcagcc ggaaaaagaa
     6301 aagaaggaat acatacaatg ctgcggtaac aactgcagca acggacagca acactgtcct
     6361 gcccggaaac aacagcacga aagccacatt gaccaggacc agaactgcaa agcctgaaat
     6421 cagtatttta tacccttccc tgtgaatgcc cattatactg aaaatttata caaaattaat
     6481 caagtcacat caatcatcca aacaatgaaa taaaaaggaa gaccagggga aaggccagca
     6541 tggtactgtc aaacctgtcc atgaatcccc cgtgccccgg cattatgctg ccgctgtcct
     6601 ttattccggt acttctttta agcatcgact cgatgagatc accgtaagta ccgcttattg
     6661 ccactatcag ggcgacaact acccatcctg cactgtcaag gataccaagc cggctgctca
     6721 gcagcgaggc ggtcagtgca gtaagaaccg taccccctat aaatccttcc caggattttt
     6781 tgggggagat cctttccatc agcctgtgtt ttcccattgt catccccgtc aggtatgcac
     6841 cggtatcatt aatccataaa aggataaaaa aacctacaac catgcccgga gaaaaatcaa
     6901 ttgactcaca tgggattatc ggtttaatac cgctgcgtga aaatgctgaa aagggaaaga
     6961 gggaaaatgg cacaaccgcg taaatcagac cgaagaatgt atgtgcaagt gaatcaaaag
     7021 gcttttcatc cctccggtaa agctcagctg tcattatcat tgcaattaag gggatcacca
     7081 gaagaaaaaa cctgtattca agcaatcctg ccgcaatcag gatggagagc aggtacacgg
     7141 caataccggt aaccatgccg gtgacagtct gaacccttgt cccgcttccg ttgattatcc
     7201 tgtaatattc ataaagtatc cctgacatta ttaccaggcc tgtcaggcaa aaggtgacag
     7261 gatgaagcca gaatccgccc aggatgaata ttacaatcca ggctccggtc agggttcgtc
     7321 tgtaaaattc cttcaaactt taaaatctgt attatttttc accggggcct tctgtaattt
     7381 cttcaggatt gccggcggca ttaaggtcag cctcttcagt ctctgttcct ttggaggctt
     7441 tggccttctt cctgttttcc ttctccttct cttcccttat cctgtcagcc ttcctcttgc
     7501 cgaatatctt tttgagatcc tcactgaata tcacttcctg ttcaagcaga agttctgcca
     7561 gtttggagag tcccttcata ttttcccgga gaacgttctt tgccctttca tacgactgtt
     7621 caattatttc ctttacttca cggtcaataa gctcggcagt cttttcactg taaggctttg
     7681 taaagccata atcggattgc cctgaagagt catagaagct tatattgcct actttgtcac
     7741 tcatcccgaa ataggaaacc atcgcatatg cctgctttgt aaccttttca aggtcgctga
     7801 gggcgcctgt ggaaattttt ccgaacacaa gctcttctgc tgcccttcct ccgagtgagt
     7861 atgccatttc atccagaagc tgttcggtgg tggtaatctg tctttcctcg ggaaggtacc
     7921 atgcggcacc cagggccctg ccccggggta ttatcgtaac tttcagaagg gggctggcat
     7981 gctcgagaag ccagctcacc gttgcatggc ccgcttcatg ataggcaatt gtccttttct
     8041 cctcgatgga aattatcttg ctctttcttt ccagtccgcc gactatcctg tctatggcat
     8101 ccagaaaatc ctgcttttcc accactgttt tatttttccg tgcagctatg agggcagctt
     8161 cgttacatac atttgcaata tccgcacctg agaaacccgg ggtctgtttt gccaggaagg
     8221 agacgtcaaa attctgctcc agcttgatag gccgcagatg cacctggaaa atggcttccc
     8281 tttcgttaag atcgggtagt tccacatgaa tctggcggtc aaacctcccg gccctcaaaa
     8341 gagcacggtc aagaatatcg gccctgtttg tggctgcaag aattatcaca cccacatttg
     8401 tcccgaatcc gtccatctcg gtcagcagct ggttaagggt gttttccctt tcgtcattgg
     8461 cactgaaatt cacatttttc ccccttgccc tgccgactgc atctatttca tcaataaaca
     8521 ctatacatgg cgctttctcc ttcgcctgct tgaacaaatc ccttaccctc gacgcaccaa
     8581 ctccgacaaa catttcaacg aaatcggaac cggaaatgga aaagaagggc acgtttgcct
     8641 ctcccgcaac tgccttggca agaagagtct tgcctgttcc gggaggaccg atcagaaggg
     8701 ctcccttggg tatctttccg cccagctgag tgtatttctt cgggttcttc aggaaatcaa
     8761 ctatctccat tacctcggtc ttggcttcct ccagacctgc aacatcattg aaattcagtt
     8821 ttacatgggt atccttgtca aatatctgtg ccctcgactt gccgacactg aaaattcctc
     8881 cggcacctcc ggctccgcca cctgtcattc tccgcatcat gaagatccat accatgatca
     8941 gcagtgcagg aagcaaaagc cagcccagta tatccgcaaa ataattcttc ctcgtaacgt
     9001 attcgggata gataagttca ttttcactgt agccggcact cttctgcgtt tcaataatga
     9061 agggctcaaa attgcttatg tcgccgatat tataggtata atggggcttg ggtacctgca
     9121 ggcccatacc gccgcccccg ggcttcggca gattctcgta ccttcccgaa ttcagcgcat
     9181 cccttgtaag atagatctca gcctgatcct tgttgacaac aaccagcttc tcgatatcat
     9241 gccttgcaat catatccttg atcatattcg ttgttgcctt ctcggttaca ttacggctga
     9301 agaaaacctg aaaaagaata aatgacaggg caatgatcgc aaaaatccag ttggtgttga
     9361 attttggctt tgctccctta tcccccggct ttccgggaga agatttattt gtattttgtg
     9421 tcatagataa attacttgct gaccttttcc tccataacct caatcaaggc atccgcccac
     9481 aatgattcaa gactgtaaaa gttcctgtat tcctccagga atatatgaac aatcacatcc
     9541 ccgtaatcta taagaaccca gaaacagttc ccgagccctt caacatgcag ggccttctcc
     9601 cccgcttcct tcctcactac atcttcaacc gactcacaga ttgaatttac ctgggtaaca
     9661 gaagttccgt gacatataac aaagaaatca gcaattctgt tctccagtcc ccgcatatca
     9721 atcctgagaa catttttccc ctgcttatcg tatatcccct ttgcaacact ctgtaaaaga
     9781 acttcagttc cggcgggcca tttcttcatt aatcactcca aattacagac caaaaaaata
     9841 aacaatttta tgaaatcaga atattccgta ccgtctgatt tcaagttgca aaaatacaaa
//
LOCUS       DAOSPX010000088         9881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6624_length_9881_cov_3.760228, whole genome shotgun sequence.
ACCESSION   DAOSPX010000088 DAOSPX010000000
VERSION     DAOSPX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6624_length_9881_cov_3.760228"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLZ75_05980"
     CDS             complement(<1..648)
                     /locus_tag="PLZ75_05980"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOS71982.1"
                     /translation="MSGIKPSRRWQPAFYPFKKEKPGARLLARVELLVKGPLWGCRMC
                     GNCLLQETAFICPMECPKGLRNGPCGGITPEKNCYVDETRKCIWYAIYSRALKKGREE
                     RLLEVLPPLDWNKTGTETWGDVIREIRKAGALKFIKGNLSSDKKKRTETRESVFRAVR
                     QPSWWNGDSEYHPPAYTEPVSGFERKLRNGEFVLTTEVSPPLGANTGHLASKIELV"
     gene            complement(649..1488)
                     /locus_tag="PLZ75_05985"
     CDS             complement(649..1488)
                     /locus_tag="PLZ75_05985"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOS71983.1"
                     /translation="MKTTLRGRTREVVIDTEGPVVIIGENINPTRRSRLVSTLQQGDF
                     EYVLELAESQISAGADILDVNVGFPGVDDVKLLPETVMRLQDRFDIPLCLDSPNPRAI
                     EAALKVAAGKCLINSVNGEEDSLNAFLPLARDYGAAIIGLCMDDDGITHDPEKRLAIA
                     GKIAERAARTGISEEDVIIDPLAMAVSADPKACLVTLETIGLVSKKLGLNITQGASNI
                     SFGLPGREFLNSAYMVLSIYNGLSCPIANPEKITPIIRAADLILDRDDYAIRFVEYFQ
                     SRQ"
     gene            complement(1485..2150)
                     /locus_tag="PLZ75_05990"
     CDS             complement(1485..2150)
                     /locus_tag="PLZ75_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOS71984.1"
                     /translation="MDDLLIKLSRCVELGKTDKSSPYPPDMKGQDGADELARQALDKG
                     ITPDSILEEALIPAMAIVGNKFSNKEIYVPQMLMAAKAMNSAMKHLKPYFQSGETRRK
                     GKFIIGTVSGDLHDIGKNLVAMMIEGGGWEVIDLGVDVSTEKFLKALEDNPGAFIGLS
                     ALLTTTMENMKKTVSAVREKYPASKILVGGAPVTMDYCQKIGADFYSPDPQGAVNYLG
                     RMV"
     gene            complement(2354..4762)
                     /locus_tag="PLZ75_05995"
     CDS             complement(2354..4762)
                     /locus_tag="PLZ75_05995"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOS71985.1"
                     /translation="MRNTKPASLILLVALIVCSCSNESGTSVQDTELWYRQPAAVWTD
                     ALPVGNGRLGGMVFGRYDHEIIQLNEESVWAGSRINNNNPEALDKLPEIQEAIFRNQF
                     KKAFELSDKYLLGTPPRIRSYQPLGHLYINFGSRGRPVSYRRSLSLNSGVAKTEYSVD
                     GNRVIQEVFVSAPHDVMVLSIKSDRNMDFDLLLSRERDTMDLPSKQGERRVPGHYINE
                     YGNENGIAFFRGQIVDFDSPLRGPGGKHMRYSAAMKLLKGGKKGLQPLISDTSAGFRV
                     QSAREVVIILTGASDYNSEKLDSDASLDPLGACLDKLSDASRCTAGKLRKIHEKDHRE
                     MFERVYFSLGDDPGRELATDERLELLKSGGSDNGLIPLYFQYGRYLLMASSRNPGVLP
                     ANLQGIWNEHYEAPWNSDFHTNINLQMNYWPAEVCNLPETSEILTGFMEKLTVPGGIT
                     AREMYGAGGWTLHHLTDPFGRTGVADGVWGITPLNGPWMTFPVYEHFLFTADTAFLEK
                     RAYPLLKGSAEFVLDFLIESPEGYLVTNPSHSPENNFRVPRTGEISALTYAATTDIEL
                     INALFDYCTEAANMLDRDHEFVSQLIAAKKKLPPIQINSRGVIQEWIKDYDEPEPGHR
                     HMSHLLGLYPLAQFTPETPELFEAARATIGRRLSFGGGHTGWSRAWIVNLFARLQDGE
                     KAYENLLELLRKSTLNNLFDTHPPFQIDGNFGGTAGIAEMLIQSHGNIIRLLPAVPRD
                     WKSGEVKGLCARGGFVIDMKWNDNRLQHVTVFSKKGGDCTLVYAGEKHKISLRAGDRE
                     TIVF"
     gene            4947..6458
                     /locus_tag="PLZ75_06000"
     CDS             4947..6458
                     /locus_tag="PLZ75_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71986.1"
                     /translation="MILFFCLFIYSLKAQESPTGIFSSSADIGNPRNSGSSVYNSSDQ
                     SYSIRGTGYNIWFERDEFHFLFNRMKGDFILTANFRFEGEGVVVHRKTGWMLRNTLDD
                     DSPHISAALHGDGLTVLQWRDSRGAPMKDPEDEIFAPGSHYEVLQLERRGKLIFMRAA
                     HPGKALEDIGSYEMINLRDEIYTGLFVCAHDPDATEEATIWNVRIDKPVSENYNPDRE
                     GYPGSRLETMNVFDGKRMVIYEKNGRFEAPNWMPDGKKLLFNMDGLLYNIPVTGGEPA
                     VLNTGFANRNNNDHGISFNGKLLAISHHREGLPGGGSTVYVLPLKGGTPKMVTENTPS
                     YWHGWAPNNKEVIYVAQRNNIPVYNIYRNSIKGGKEVALTNIEKGQHVDGCEYSPDGR
                     YIYFNGNLNGTMQIWRMKPDGSSMEQMTSDGYNDWFPHISPDGKWMVFLSFPPDIEPN
                     SHPSYKRVMLRMMPASGGEPVVIAWLYGGQGTINVPSWSPDSREIAFVSNSGK"
     gene            6473..7669
                     /locus_tag="PLZ75_06005"
     CDS             6473..7669
                     /locus_tag="PLZ75_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71987.1"
                     /translation="MTKKTVRCGIAGSGFAAKFHYEALLRVFSVKVDLAGSYSPTRER
                     LEQFTGPRNLKAYSSIDELIDDVDVVHVCTPPSTHEPIVVAALEKNRHVIVEKPLTGY
                     FGDGSPVFHGDTFPREKGLSEALASCGRMLEAEKRSRGRIMYAENWIYAPAIMKERDM
                     LRKTKAQIIWMHGEESHSGSHSLSYGQWKFSGGGSMIGKGCHPLSAALYFKQVEGLAR
                     TGEPIRPVAITARTHAVTRMPGYMDQGHLRTTYTDIEDFALMHVVFADGTFADIFASE
                     LVLGGVHNWIEVNTNNHRTICNISPNNAMQAYTPDEKHFRDVYIVEKTETKQGWSSVS
                     PDEGWFTGYQHEMEAFYRTAAFGDPVESNSILAADTIATIYAGYLSAEKKGAEVAVTL
                     HQDRPA"
     gene            complement(7789..8685)
                     /locus_tag="PLZ75_06010"
     CDS             complement(7789..8685)
                     /locus_tag="PLZ75_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS71988.1"
                     /translation="MNRRQFTKRTVMASAVASFAPVQSFSKLLSPPARAIKKGIMWGS
                     NGVGKTVAEKFRSAKAAGFDGIEPMSHLNRDEVLRAMKETGLSVPSVCGEMHWKYLLS
                     DPDPAIREKGVEALKLSIEDASAYGADTVLLVPGRVSETVTYDECWKRSTDEIRKVIP
                     LAEKLKIRIAIENVWNNFLLSPLEAANYVDQFQSEAVGFYFDCGNILVYGWPEQWIRI
                     LGKRIAKVHIKEYSRKIGDSQGKSAGFRVDLLEGDVNWPEVMKALDDIGYSGWATIEQ
                     PGGNTAEGLKDLCDRLIRIIAS"
     gene            complement(8819..>9881)
                     /locus_tag="PLZ75_06015"
     CDS             complement(8819..>9881)
                     /locus_tag="PLZ75_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS71989.1"
                     /translation="NCRAQLKKQKNVEIADDKCFVGFDCYQKVIDSGVDLVLLCTPPH
                     FRPAHIEAAVNADKHIFLEKPCAVDPVGARSVLVSVEKAKQKGLCMVSGTIYRVSKDY
                     METRRRVMNGAIGDIVSAHVIRNGGSLWVRSRQPGWTDMEYMLRNWGNFCWLSGDHIV
                     EQFIHQIDVMNWYMGENPVRAIGWGGRQRRVTGDQYDFFSIEYVYENGMHTHCAARQI
                     SGCSNLTRVHIAGTEGYADATGAIYNWKGEEIWKYPRPEKDDPDQTWAVKDPFVQEHI
                     NLVTGIRTGNVVNDAEAQVMSTLVAVMGRMSAYTGRDVTWEEVINSDMRLGPTVYEFG
                     PVEGIPETIPVIGIEAKVG"
BASE COUNT         2559 a         2547 c         2177 g         2598 t
ORIGIN      
        1 gacaagttca atcttacttg caagatgacc ggtattcgcc cccaggggag gagaaacctc
       61 ggttgtcaga acgaattctc cattgcgtag ttttctctca aatcccgaga ccggttcggt
      121 ataggcagga ggatggtact ccgaatcacc gttccaccat gaaggctgtc tgaccgccct
      181 gaaaacggat tcccgggtct ctgtcctttt tttcttatcc gatgaaagat ttccttttat
      241 gaatttgagt gcacctgctt tccttatctc tcttatcaca tctccccagg tctcggtacc
      301 ggtcttgttc cagtcaagag gaggcagaac ttcaagaagt ctttcctccc ttcccttttt
      361 aagggcccgg ctgtagattg cgtaccagat acatttgcgg gtttcatcaa catagcagtt
      421 cttttcgggt gttatacccc cgcagggacc attcctcagc cccttggggc attccatggg
      481 acagatgaat gccgtttcct gcaaaagaca gttcccgcac atcctgcatc cccacaacgg
      541 ccccttgacc agaagttcaa ccctggcaag aagccttgcc ccgggttttt cttttttaaa
      601 cggataaaaa gccggctgcc atctcctgga aggttttata cccgacatct attgcctgct
      661 ctggaaatat tcaacaaacc tgatggcata atcatccctg tcaagtatca gatcggcagc
      721 ccgtattatc ggagtaatct tttcaggatt tgcaatcgga cagctaaggc cgttatagat
      781 tgacaggacc atgtaggcag aattcaggaa ttccctgccg ggcagaccga aggatatgtt
      841 gcttgctccc tgtgtaatat taagtccaag cttcttgctg acaagcccga tagtctcaag
      901 cgtaaccagg caggctttgg ggtcggcgct tacagccatg gcaagagggt caataataac
      961 atcttcttcg ctgatgccgg tcctggctgc ccgttcggct atctttccgg caattgccag
     1021 tctcttctcc ggatcatggg tgattccgtc atcatccata cacaaaccta ttattgcagc
     1081 accataatcc ctggcaagcg gcaggaaggc attcagggaa tcctcttccc cattcaccga
     1141 atttatcagg catttgcccg cggcaacttt cagtgctgct tcaatggccc gcggattggg
     1201 cgaatcaaga cacaggggaa tatcgaacct gtcctgcagg cgcatgactg tttccggcag
     1261 aagcttcaca tcatcaaccc cggggaatcc gacgttcaca tcaagtatat ccgcaccggc
     1321 actgatctgg ctttcggcaa gttcaaggac atattcaaaa tctccctgct gaagggtaga
     1381 aacaagcctg ctccttcttg tcggattgat attctcaccg ataatgacaa cagggccctc
     1441 tgtgtcaata acaacttccc gggtcctgcc ccttagtgtt gtcttcatac catccggccc
     1501 agatagttca ccgcaccctg cgggtcgggc gagtagaaat cggcgccgat cttctgacag
     1561 tagtccatgg tcaccggcgc accacccaca agtatttttg atgcaggata tttctccctg
     1621 acagctgata ctgtcttttt catattctcc atggtggtgg tcagaagagc agaaagtcct
     1681 atgaaagccc cgggattatc ttcaagtgcc ttcaggaact tctctgttga tacatcaact
     1741 ccgagatcaa tgacttccca gcctcctccc tctatcatca tggcaacaag attcttgcct
     1801 atatcgtgca gatcgccgga tacggttccg ataatgaatt taccttttct cctggtttca
     1861 cccgactgaa aatagggttt gagatgcttc attgcactgt tcatggcctt ggcggccatc
     1921 agcatttgag gaacgtatat ctccttgttg ctgaatttat tgcccactat tgccattgcc
     1981 ggaataaggg cttcctcaag tatggaatca ggtgttatac ctttgtccag agcctgtctg
     2041 gcaagctcat cggcaccatc ctgacctttc atgtcggggg gatagggtga ggatttgtcg
     2101 gttttcccca gctcaacgca acgggaaagt ttaatcagaa gatcatccat atgtggtatt
     2161 atttgaatta atatacagaa aatctgaagt aatctataaa ctgttaaaaa tatccaattt
     2221 tatctaatca attgctttat tgcatgtgaa aattatcccg taaatagtag aatcccttca
     2281 ggattacgaa acttcatcat cgtcttatca aaaggattaa tcctctcaaa gaaaatcagg
     2341 aaaattctga cagctaaaaa acaatggttt ccctgtctcc tgccctcaag cttattttat
     2401 gtttctcccc ggcataaacc agagtgcagt caccgccctt ctttgaaaaa acagtcacgt
     2461 gctgaagcct gttatcattc catttcatgt caatcacaaa acctcctctt gcacacaggc
     2521 ctttcacctc accgcttttc caatcacggg gaactgccgg aagcagtctt ataatattgc
     2581 catgcgattg tatcagcatt tcagcaattc cggcagtgcc tccaaagttg ccgtcaatct
     2641 gaaagggcgg atgggtgtca aaaagattat tcagggttga tttcctcaat aattccagga
     2701 ggttttcata ggctttttcc ccgtcctgca gacgtgcaaa cagattgaca atccacgccc
     2761 tgctccatcc cgtatgcccg ccgccgaatg aaagccttct ccctatcgtc gccctggcgg
     2821 cctcaaaaag ttccggagtt tcaggagtaa actgagccag cggataaaga ccgagaaggt
     2881 gtgacatatg acggtgcccc ggttcaggtt catcataatc ctttatccat tcctgaatga
     2941 ctcccctgga gtttatctga atcgggggaa gtttcttttt ggcagcaata agctgagaga
     3001 caaactcatg atctctgtca agcatatttg ctgcctctgt acaataatca aacagggcat
     3061 taataagttc aatatcggtt gtggctgcat aggtaagtgc tgaaatttct cctgtccggg
     3121 gaactctgaa attgttttca ggggaatgtg aaggattggt aaccagatag ccctccggcg
     3181 attcaataag gaaatccagt acaaattcag cggagccttt aagcagcgga taggcccttt
     3241 tttcaagaaa tgcagtatct gccgtaaaca ggaaatgttc gtatacagga aatgtcatcc
     3301 agggaccgtt aaggggagtg attccccaga caccgtctgc cactcctgtt ctgccgaaag
     3361 gatcagtaag atgatgcagg gtccatcctc ccgcgccata catctccctg gcagtgatcc
     3421 cccccggaac cgtcagtttt tccatgaatc cggtgagtat ctccgacgtt tcaggcaggt
     3481 tacaaacttc tgcaggccag tagttcatct gaagattaat attggtatga aaatcggaat
     3541 tccagggtgc ttcataatgc tcgttccaga ttccctgaag gttggcaggc aataccccgg
     3601 ggtttctgga agaagccata agaagatagc gtccgtactg aaaatacagc ggaataagcc
     3661 cgttatcact gcctcccgac ttaagtaatt ccagacgttc atcggtagcc agttcccggc
     3721 cgggatcatc tccaagtgaa aaatacaccc tctcaaacat ttccctgtga tccttttcat
     3781 gaatctttct cagcttcccg gcagtgcaac gggatgcatc tgaaagttta tccagacagg
     3841 ctccaagagg atccagcgaa gcatcagaat caagtttttc cgagttgtaa tcgctggcac
     3901 ccgtaagaat aataacaacc tctcttgccg actgaacacg gaatcctgcc gaagtatccg
     3961 atataagggg ctgcaggccc tttttcccgc cttttaaaag cttcatggct gcagagtatc
     4021 tcatgtgctt tccccccggc cccctgagag gtgaatcaaa atctacaatc tgacccctga
     4081 agaaagctat gccattctcg tttccgtatt catttatgta atgacccggc acccgtctct
     4141 caccctgttt cgaaggcaaa tccatagtgt cccgctcccg ggaaagaagc aggtcaaagt
     4201 ccatattcct gtctgattta atgcttaaca ccattacatc atggggagct gaaacaaata
     4261 cttcctgaat aaccctgttc ccgtcaactg aatattctgt ttttgcaacg ccgctgttaa
     4321 ggctgagcga tcttctgtag gaaacgggcc tgcccctgct gccgaaattg atataaagat
     4381 ggccaagcgg ctgataggat cttatccggg gaggggttcc cagcaaatat ttatcagata
     4441 gttcaaaagc ctttttaaac tggtttctga atatcgcctc ctgtatttca ggaagcttgt
     4501 ccaatgcttc aggattattg ttatttatcc ttgatcctgc ccagacactc tcttcgttaa
     4561 gctgtatgat ctcatgatcg tatctgccaa ataccattcc gcccaggcgg ccgttaccga
     4621 ccggcagtgc atccgtccag acggcggcag gctgtctgta ccacagctcc gtatcctgaa
     4681 cagaggttcc tgattcattg ctgcaggaac atacgattag ggcaacaagt aatataagac
     4741 tggcgggttt agtgtttctc atttctgaaa attctgatta aaggttgaat tctgcttata
     4801 aatctaaata aatattataa tatcgttatc attgtcccgg aaaatctcca aacagttatt
     4861 gaaggattgc atctgttaat tgcaaagtga taatttaaaa attcataatt ttataccttt
     4921 tagcccttat gcacatgaaa aacacaatga ttttattttt ctgcctcttc atttacagtt
     4981 tgaaggcaca ggaatccccg acaggcattt tcagctcatc agccgatata ggcaatccgc
     5041 ggaacagtgg ttcctcggta tataacagca gtgatcagag ctattcaatc aggggaacag
     5101 gctataacat ctggtttgaa agagatgagt ttcatttcct tttcaacagg atgaaaggtg
     5161 attttattct gacggcaaat ttcagatttg aaggagaagg tgttgttgtt cacagaaaaa
     5221 caggctggat gctgagaaac accctggatg atgattcccc ccatatttca gcagcactgc
     5281 acggtgacgg tctcactgta ctgcaatgga gagacagcag gggagctccg atgaaagatc
     5341 ctgaggatga aatttttgcc cctggttcgc attacgaagt cctccagctg gaacgccggg
     5401 gaaaactgat ctttatgagg gccgctcatc cgggaaaagc cctggaggat ataggatcct
     5461 atgaaatgat caacctccgg gatgaaatct acacaggcct ctttgtatgt gcgcatgacc
     5521 ccgatgcaac agaggaggca accatctgga atgtgcgcat tgacaaaccc gtaagtgaaa
     5581 actataatcc cgacagggaa ggatacccgg gcagccgtct ggagacaatg aatgtttttg
     5641 acggaaaaag aatggtcatc tatgaaaaaa acggcaggtt tgaagccccg aactggatgc
     5701 cggacgggaa aaagctgctg ttcaacatgg acgggcttct ttataatatt ccggtcacgg
     5761 gaggagaacc cgctgtatta aacaccggat ttgccaaccg caacaataat gaccacggaa
     5821 tatcattcaa cggcaagctg cttgccatca gtcatcaccg tgaaggatta cccggaggcg
     5881 gatcaacagt gtatgtcctt cccctgaaag gaggcacccc gaaaatggtt actgaaaaca
     5941 ccccttccta ctggcacgga tgggccccga acaataagga agtgatatat gtggctcaaa
     6001 ggaacaatat ccctgtttac aatatttaca ggaattcaat aaagggagga aaggaagttg
     6061 cccttaccaa tatcgaaaag ggtcagcatg ttgacggatg cgaatattct cccgacggca
     6121 ggtatatata tttcaacggc aacctcaacg gcaccatgca gatctggaga atgaaacccg
     6181 acggatcatc aatggaacag atgacctccg acggatacaa tgactggttc ccgcacattt
     6241 cccctgacgg gaaatggatg gtgtttcttt catttcctcc ggacatagag ccgaattccc
     6301 atccttcata taaaagagta atgctcagaa tgatgccggc atcaggagga gagcccgtag
     6361 ttatcgcatg gctttacggg ggccagggaa ccatcaacgt cccgtcatgg tcacccgaca
     6421 gccgggagat agcctttgta agcaattccg gcaaataaaa aatattcatc ccatgacaaa
     6481 aaagacagtc cgctgcggaa ttgcaggttc cggatttgcg gcaaaatttc attacgaagc
     6541 attactgagg gtcttcagcg taaaagttga tcttgcaggc tcttattccc cgacacggga
     6601 aaggcttgaa caattcaccg ggccccggaa cctgaaggct tacagcagca ttgatgaact
     6661 gattgatgat gttgatgttg tccatgtctg tacccctcct tcaacacatg agccaatagt
     6721 tgttgcagcc cttgagaaga acaggcatgt tattgtggag aaacccctta caggatattt
     6781 cggagacgga agccctgtct tccacggtga tacatttcca agggagaagg gtctcagtga
     6841 ggcacttgcc agttgcggga gaatgcttga ggcagaaaaa cggagcaggg gaaggatcat
     6901 gtacgccgaa aactggattt atgctccggc catcatgaag gaacgcgaca tgctccgcaa
     6961 aacgaaggca cagataatct ggatgcatgg cgaggaatcc cactccggat cccactccct
     7021 gtcatacggg caatggaaat tctcaggtgg cggatcgatg atagggaaag gctgtcatcc
     7081 cctttcggcc gcactgtatt tcaaacaggt tgaaggcctc gcaaggactg gtgaaccgat
     7141 aaggcccgtt gccataacag cccgtactca tgctgtgacc aggatgccgg gctatatgga
     7201 tcagggacat ctcagaacca catacaccga tattgaagac tttgctctga tgcatgttgt
     7261 ttttgctgac ggcaccttcg cggatatttt tgcaagtgaa ctggtactcg gaggagtcca
     7321 caactggata gaagtgaata ctaacaatca ccggacaatc tgcaatatca gtcccaacaa
     7381 tgcaatgcag gcatacacgc ccgatgagaa gcacttcagg gatgtttaca tagtcgaaaa
     7441 gacagaaaca aaacagggat ggtcatccgt ttcgcccgac gaaggctggt tcaccggtta
     7501 tcagcacgaa atggaagcat tctaccgtac agctgcattc ggggatcctg ttgaaagcaa
     7561 cagtattctc gccgccgata caattgctac gatctatgcc ggctatcttt cggccgaaaa
     7621 aaagggtgca gaagttgcag ttacccttca ccaggacagg cctgcatgat attcaggcag
     7681 ctgacagtat tttcagctgg ccatattttc agctggcaat actctctgct ggccgttttc
     7741 tcagctggca atattttcag ctggccgttt tctcagctgg ccctgttctc aactggctat
     7801 tatgcggatc agcctgtcac acagatcctt cagaccctca gctgtgtttc cgcccggctg
     7861 ttcaatggtt gcccagcccg aataacctat gtcatcaagg gctttcatca cttcaggcca
     7921 gttaacatca ccctcgagca ggtcaaccct gaatcctgcc gatttaccct gactgtcacc
     7981 tatcttccgg ctgtattcct tgatatgaac cttggcaatc cttttgccca ggatccttat
     8041 ccactgctcc ggccagccat aaaccaggat attgccgcag tcgaaataaa acccgacagc
     8101 ctcactctgg aactgatcaa cataatttgc agcttccaga ggactcagca ggaaattgtt
     8161 ccagacattc tcgatcgcaa tcctgatctt cagtttttcc gccaggggaa taacctttct
     8221 gatctcatcc gtggatctct tccagcattc gtcatacgtt accgtttcac ttaccctccc
     8281 gggaacaagg agtactgtat ccgccccata ggcactggca tcttcgattg acagcttcag
     8341 ggcctcaaca cctttttcgc gtatcgcggg atccggatcc gacagaaggt acttccagtg
     8401 catttcacca caaacacttg gaacagataa accggtttct ttcatagccc ttagaacttc
     8461 atcccggtta aggtgactca ttggttcaat gccatcgaaa cccgcagcct ttgcagaccg
     8521 gaatttctct gcaacggttt ttccgactcc gttgctcccc cacattatac cttttttaat
     8581 ggctctggcg ggaggggaaa gcagcttcga aaagctctgt accggtgcaa acgatgccac
     8641 agctgacgcc atgacagtcc gttttgtaaa ctgacgtctg ttcatataga attaatttat
     8701 tgtaatttaa gaatttcctg ttacatttcc cgtccttccc cacaatgtct cagcggagaa
     8761 gaagcatacc ctgtcaggat gaaaacaacg gttctgcttc tgatctgccg ttaaagaatc
     8821 aacccacctt tgcttcaata cctatcacgg gaatggtttc cggtatgcct tcaaccggtc
     8881 cgaattcata gaccgtcggt ccaagccgca tgtcggaatt gatcacttcc tcccaggtaa
     8941 catccctgcc ggtatatgcc gacattctcc ccataactgc aacaagggtc gacatcacct
     9001 gggcctcagc atcgttaaca acatttcctg tgcggatgcc ggttaccaga tttatatgtt
     9061 cctgaacgaa aggatccttg actgcccagg tctgatcagg atcatccttt tccggcctcg
     9121 ggtatttcca tatttcttcc cccttccagt tgtaaatggc tcctgttgca tctgcatatc
     9181 cctctgttcc ggcaatatgc acgcgggtaa ggtttgaaca tcctgaaatc tgcctggctg
     9241 cacagtgtgt gtgcattccg ttctcataca cgtattctat actgaaaaaa tcatactgat
     9301 caccggtaac ccttctctgg cgtccgcccc agcctattgc cctgaccggg ttctccccca
     9361 tataccagtt catcacgtca atctgatgaa tgaactgttc aacaatatga tctcctgaaa
     9421 gccagcagaa attgccccag tttctgagca tgtattccat gtcggtccag cccggctgcc
     9481 gggatctgac ccacagggaa ccaccgtttc ttattacatg ggcacttaca atatctccta
     9541 ttgccccgtt cattaccctt ctcctggttt ccatatagtc ttttgatacc cggtatatag
     9601 taccgcttac catgcacagg cccttctgct ttgccttctc caccgacaca agaacagatc
     9661 ttgccccgac aggatcaacg gcacagggtt tttccaggaa aatatgcttg tctgcattta
     9721 ctgcggcttc aatgtgtgcc ggcctgaaat gcggaggggt acagagaaga acaagatcaa
     9781 cacccgaatc tattactttc tggtaacagt caaaccccac aaaacatttg tcatccgcta
     9841 tttccacatt tttctgcttt ttaagctgtg ccctgcagtt g
//
LOCUS       DAOSPX010000089         9837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6655_length_9837_cov_4.778062, whole genome shotgun sequence.
ACCESSION   DAOSPX010000089 DAOSPX010000000
VERSION     DAOSPX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6655_length_9837_cov_4.778062"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLZ75_06020"
     CDS             complement(<1..836)
                     /locus_tag="PLZ75_06020"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS71990.1"
                     /translation="MKIMNKNIIILLLVIIGSSCTDFLDEKPKDRLTEINFYETFADA
                     QAAVNAIYGPIRNNYRDTYFLMLDLLADYLEGRGSTSPVSEYKGFDVTNINRAAAAWR
                     YLYQTIRNANIAIERIPKIEGMKESDKNALIAEASFLRAFSYYFLVRLWGAVPLYLDL
                     SPVDLSRRPVSEVYQAIINDLKTGETYLPDTPSEFGHPTKWSAKSLLAEVYLTINEFD
                     LSKDKAGEVITSGHFNLVDVNVANDFNNVYGPEANGTPEEIFYIKFNHTAGWTMPHKY
                     LW"
     gene            complement(858..3830)
                     /locus_tag="PLZ75_06025"
     CDS             complement(858..3830)
                     /locus_tag="PLZ75_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS71991.1"
                     /translation="MKKLFLVLFLIIISSGFLVAQREITGKVNDEDQNPLSGVSVVVA
                     GTNVGTLTDINGQFSLQIPPNATTLVFTFIGMKRVEMEIGNLTVFNVTMSFDVGLLDE
                     VIVIGYGTIKKSDLTGSISSVKGDNIRLQAVGNVVQSLSGVAAGVQVLQTSGQPGSGT
                     NVVIRGGNSLLGDNNPLYIVDGTPMITDLSMFNTNDIESIEVLKDASGTAIYGSRGAN
                     GVVLVTTKKGKSGGLSVEYSGYYGVQQVTKKIEMLNAKEFASLVNERYLNDGLSPYFS
                     SSEIESFGEGFNWQDAVFKVAPIQNHSLRLSGGSDKTTYSISGSYFGQDGIIINSGYN
                     NLQFRSRIDTKITKDWSFAVNTVLSRRMNNNIHSNNTERGMGVLSAAVGCPPTIPAYD
                     ANGDYSFMMAYPFSPEGLENPIAMAYERIMKNHSNDLSLNLSTSANIFKDLTLYSSIG
                     IQYDNAREDYYSGTKLFASRTGMANIGYSELMNILNENTLTYKKVIGTNHDLTILGGV
                     TAQKNTAQNLRAGSTGFLTDILQNYSLQSGENFQTPTSDYSDWTLLSYISRINYSFKS
                     KYLFTASIRADGSSRFGSNNRWGYFPSFAVGWNINEENFLKGNPTLNILKLRMSWGQT
                     GNTSITPYQSLSVLSSIQTVFDKNLNIGYAPGNIKPNPDLKWETTAQSNIGLDVELFN
                     NRLSLSSDFYYKKTRDLLVNIPVFPSTGYASQTTNLGNIENKGLEMALNWRVFDQKFL
                     WNLGSTFTLNRNKVLSLPQGGDIFGTEIGWVLPAMNLVREGYPVGVFYGYIEDGLTED
                     GNIKYLDLHPDGTINTLDRTIIGDPNPKFIFGINSDMSYKNFGLTVLINGVQGNDILN
                     YNLSNIGDSFSYGINQTKDIIGNYWTSQNPNPNAKYPRISKNTRFLGSDRFVEDGSYV
                     QVKNIKLSYSLSGDKFKASRIHNSQVYISLQNIITLTKYTFYTPIQNTLGTGINKGID
                     KWGYPDARLFMLGVMVNL"
     gene            4486..4938
                     /locus_tag="PLZ75_06030"
     CDS             4486..4938
                     /locus_tag="PLZ75_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71992.1"
                     /translation="MKKILFISMALSVLLLNGCKKEETGLSEFILGDWATDKLTFSEN
                     QKGIIHITFQDNNEYELSIEMIPGHKNGSIKGQFEVDNKTRMLTLDSDAIIRLLGLET
                     SGDPIMFNVEFSTTSDVDEMTLRLASLPEAVGEFVPSIVLKRVSGQTS"
     gene            5278..5757
                     /locus_tag="PLZ75_06035"
     CDS             5278..5757
                     /locus_tag="PLZ75_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71993.1"
                     /translation="MKRLFFTCLTAAAIILASCGGGTTQKEGATQKTGSTDEKKVQTE
                     AKANYLDREVLEAKLKEIGFPIYPGAEFEKIEKGSGYFKGAYIQYEIPEVSSESKEAV
                     DKFYKEVFEKLEASGWKNVFDVYWEKRDMLLHFAHVYNDFQAETHFLGITFGLSSEQ"
     gene            complement(5843..6664)
                     /locus_tag="PLZ75_06040"
     CDS             complement(5843..6664)
                     /locus_tag="PLZ75_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS71994.1"
                     /translation="MKAVFFLHIAVIMLISASCSGYYGHDIDLSATYSGAVCSPNSSE
                     IYFLCQTRASQKGKNLWFIMPIEGKTRFLHNNVSLYSFDTTGIRLTRLFDYGNLPNYL
                     SSWKAEIIPLKDGVCFSISPKLGGWDEMVSKDSNMVSVRQQFEGIFFIDRDGEVKRLD
                     SQPPADDVVKPEKGVRFYVGNLPYSEFGLILQEFDPGTEKYYLKTLEQLGNTSTYRRA
                     VIEQVLAVKDKNTISKVYDNMLRNLNEMKEGSKKMMKEMDIKDNLEQIQALLQSK"
     gene            7174..7887
                     /locus_tag="PLZ75_06045"
     CDS             7174..7887
                     /locus_tag="PLZ75_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOS71995.1"
                     /translation="MTMRKIAIMLAVILLTPACGKKTPQPGNEDLEGIWECIKGCEAE
                     MLEFNIEDERQVFFFYTGQRMYRSGTWKLEKNRLTLAYDDEETLTYPLALKNDTLVLG
                     DNKMVFTACIPAEEDPCDLSSLFALMDDISFSQPLLEDFPWNIPGDSGVEEVIVNGQM
                     VETIVKLEGDFSAIGRTAGVIADELRKLGYEADPLMASEVVTGYRKENCVVLFRPGID
                     DEDDADAEVPFAVYFGKLP"
     gene            8605..>9837
                     /locus_tag="PLZ75_06050"
     CDS             8605..>9837
                     /locus_tag="PLZ75_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOS71996.1"
                     /translation="MNTSFYNNILTAFIFMIMLVISLPEVQAQDYRIAFAGSGASTTV
                     DSVRVENLTTGTIVRLNGTDLLHLGTVSGIQDLNDTRKEEINFYPNPMPEYSVMHFRL
                     PVSGSTLIEIRDISGKTICQKRETLSQGVHSYRISGMGKGIYLVSISSPGHVITGKLV
                     SACPNSGKINIMREESIPAGGKTTDSKGTAEEVFMPFTAGDRLKITAISDKYRTVIVD
                     SPEGDKTIVFTFIECTDGDGNNYPVVVLENEEGKNDGGKSGTGKGKPALMAENLKTTK
                     FNDGTDIPEKRGSEWDTPGPAYCWYNNNRYYNENRYGALYNYAAFTSGKLCPAGWHAP
                     ADEEWNDLWDYLGGGNVGQKLKARWDTYWNDGGGTDEIGFGARGGGIRLGNNPYIDFD
                     LQKEWCYFWCIGFTLGNEA"
BASE COUNT         3024 a         1985 c         1964 g         2864 t
ORIGIN      
        1 tcccacaaat atttatgagg cattgtccag ccggccgtat gattgaattt tatataaaat
       61 atttcttcgg gagtaccatt tgcctcagga ccgtaaacat tattaaagtc atttgcgaca
      121 tttacatcaa caaggttaaa atgacctgag gttataactt ctcctgcctt gtctttcgac
      181 agatcaaatt cattaattgt aaggtatacc tcagcgagca atgatttggc agaccattta
      241 gtgggatgtc caaattcaga tggtgtgtca ggtaaatatg tttctcctgt ttttaagtca
      301 ttgatgattg cctgataaac ttctgaaact ggtcttctgc ttagatctac cggagaaaga
      361 tctaaatata acggaactgc tccccaaagc cttacaagaa agtaatatga gaatgcacgc
      421 aaaaaagagg cctctgctat taatgcattt ttatccgatt ctttcatacc ttcaattttc
      481 ggaattcttt ctatggcaat atttgcattc ctgatcgttt ggtaaagata tctccatgca
      541 gcagccgcac gatttatgtt agtcacatca aaacctttat actcgcttac cggggatgtg
      601 gaaccacgtc cctcaagata atcagccagc aaatcaagca tcaaaaaata agtatctctg
      661 taattattcc tgattggtcc gtagattgcg tttacagctg cctgagcatc tgcaaaagtt
      721 tcataaaaat taatttctgt cagacgatct ttgggtttct catcaagaaa gtcggtacac
      781 gatgatccta tgataaccag taatagtatt attatatttt tattcatgat tttcatatta
      841 attatattaa taatagatta taaattaacc atgactccta acataaataa ccttgcgtca
      901 ggataacccc atttatcaat tcctttattg ataccagttc ctagggtatt ctgtattggt
      961 gtataaaaag tatactttgt taaagtaatt atattttgca ggctaatata aacctgggag
     1021 ttatgaatcc tggatgcctt aaatttatct ccggacaaag agtaggacaa ttttatattc
     1081 ttaacctgga cgtaggagcc atcctctaca aatctatcag agccaagaaa tcttgtattt
     1141 ttgcttattc gaggatattt tgcattagga tttggattct gactagtcca ataattccct
     1201 atgatgtctt tggtttgatt aattccgtaa ctgaaactgt ctccaatatt tgacaggtta
     1261 taattcagaa tatcattccc ctgaacacca tttattaata ctgtcagtcc aaaattttta
     1321 taggacatat cagagttaat cccaaaaata aattttgggt ttggatcgcc aataattgtc
     1381 ctgtccagcg tattgatcgt tccatcaggg tgtaaatcaa ggtattttat atttccatct
     1441 tctgttaatc catcctctat atatccataa aatacgccaa ccggataacc ttccctaacc
     1501 aggttcatgg caggaagcac ccagccaatt tctgtaccaa aaatatcgcc accttgagga
     1561 aggctcagca ccttattgcg atttaaagta aaagttgatc ccaagttcca taaaaacttc
     1621 tgatcaaaaa ctctccagtt caatgccatt tcgaggcctt tattttcgat attccccaga
     1681 ttagttgttt gggaagcata accagttgac ggaaataccg gaatattaac caacaaatct
     1741 cttgttttct tataataaaa atcagatgaa aggctgagtc tattattaaa taattccaca
     1801 tccaaaccaa tattggactg agctgttgtt tcccacttaa gatcgggatt aggcttaata
     1861 ttgccaggcg cataaccaat attaagattt ttatcaaaaa cggtctgaat acttgacaga
     1921 acagaaagtg attggtaggg tgtaatagat gtattaccag tctgtcccca gctcattctc
     1981 aatttaagaa tgtttaatgt tggattgcct ttcaggaaat tttcttcatt aatgttccag
     2041 cctactgcga atgaaggaaa ataaccccat ctattatttg atccaaagcg tgaggaaccg
     2101 tcggctctaa tactggcggt aaaaagatat ttacttttaa aggaatagtt tatcctgctt
     2161 atataggaca atagggtcca atcactatag tctgaggtgg gtgtctggaa gttttctcca
     2221 gactgaaggc tgtagttctg aagtatatct gttaaaaaac ctgttgaacc agctcttaaa
     2281 ttctgtgcag tattcttttg tgcggtaacc ccacccaaaa tggtcagatc gtggtttgtt
     2341 cctatcactt tcttataggt cagtgtattt tcattcaaaa tgttcattag ttccgaataa
     2401 cctatatttg ccattcccgt tcttgatgca aaaagcttgg ttccagaata atagtcttct
     2461 ctcgcattat catattgaat tcctatcgac gaatataatg tgagatcctt aaaaatattt
     2521 gcactagtag aaaggttcag acttaaatca ttggaatgat ttttcattat gcgttcatag
     2581 gccatggcaa taggattttc caagccttct ggactgaaag gataagccat cataaaacta
     2641 taatctccat tggcatcata tgccggaatg gttggaggac aacccacggc agcactcaat
     2701 acccccatac ctctttcagt attgttacta tgaatattat tattcattcg cctgcttaaa
     2761 actgtattaa ccgcgaacga ccagtctttg gttattttag tatcaatcct tgatctgaac
     2821 tgtaagttat tgtatcctga gttgataata attccatcct gtccgaaata gctaccagat
     2881 atactataag tggtcttatc agatccaccc gacaatctaa gggaatggtt ttgaataggt
     2941 gcaactttaa atacagcatc ctgccaattg aatccttctc cgaatgattc aatttccgat
     3001 gatgaaaaat aaggacttaa accatcattt aggtatcttt catttaccag cgatgcgaat
     3061 tcttttgcat taagcatttc aatctttttt gtcacctgct gtactccgta atatccactg
     3121 tattcaacac ttagtcctcc tgatttaccc ttcttagtag tcaccagaac cacaccatta
     3181 gcacctcgtg agccgtaaat tgcagtaccg gatgcgtctt tgagaacctc aattgattct
     3241 atatcattgg tattaaacat actcagatcg gtaatcattg gggttccgtc aacaatataa
     3301 agaggattgt tatcacccaa aagtgagttg cctcccctta taactacatt ggtcccacta
     3361 cctggttgtc ctgatgtttg aagcacctgt acacctgcag ccacaccgct aagtgattga
     3421 actacattac ccacagcctg caatctgata ttatcacctt taactgaaga tattgaaccg
     3481 gttaaatcac ttttctttat tgttccatag ccgataacta ttacctcatc aagtaacccc
     3541 acatcaaagg acattgttac attgaaaacc gtcagatttc caatttccat ctccactcgt
     3601 ttcatcccaa taaaggtaaa aacgagtgtt gtggcgttag gtggaatctg aaggctgaat
     3661 tgaccattga tatcagtaag agttccgaca ttagttcctg caacaactac agatacacca
     3721 gacaaaggat tctgatcttc atcatttacc tttccggtta tttctctttg tgccacgagg
     3781 aaaccagagc tgattataat taaaaaaagt acaagaaaaa gttttttcat agtaaaagat
     3841 agttaagtta ttaattataa ataatagtag tctctttact aattagtatg tcctcacaat
     3901 ttgggtttaa gattgcaagc ttagctcaga acgtattcaa cggttactat cacaatcgag
     3961 gtaaatataa agattttttg atatagtttc ttctttagga tataaaaatt ccgagaacaa
     4021 atccccctat tggccgatat aaggtaatga tccttcattc gactaaaaat agagggtttc
     4081 atatgtcatt atctgcaatg gaaaaggctt tctgctttat aataattcag aggaaaagat
     4141 cattttaata ttttaggaaa cgattaaata tacttaaggt caaacgtttt aattaaattt
     4201 agtattaagc atttaagata aatccggaaa cagggatcat ctttcaaaat ggtaagcatg
     4261 cttgcaaaat ccgtttgccg ttttggtcag ggaacagtct taaaatcaag gcaacaaata
     4321 ttagcgttca acgtaaaagg atcaatgtaa aatcttttaa atggttaata tcagattatt
     4381 gcattacaat catcagacac tttcagtggt ttttgaaaaa tcaaaatata taattccgga
     4441 tttaatattg aaaattatta tttatcaatt taaaacacat tgattatgaa aaagatattg
     4501 tttatctcaa tggctctttc tgttcttttg ctgaatggct gcaagaaaga ggaaacagga
     4561 ctctccgaat ttatcctggg tgactgggct actgataaat taaccttctc tgaaaaccag
     4621 aaaggtataa tccatataac attccaggat aacaatgaat atgaactgag catcgaaatg
     4681 atcccgggac acaagaatgg ttcaatcaaa ggacaattcg aggtagataa taaaaccagg
     4741 atgctcacgc tggacagcga tgcaataatc agattattag gactggaaac ttccggtgat
     4801 ccgatcatgt tcaatgtgga gttcagtacc acgagtgatg ttgatgaaat gacccttagg
     4861 ctggcatccc ttccggaagc agtaggtgaa ttcgttccat caatagttct caaaagagta
     4921 agcgggcaga cttcctgacc ccgaccttta cttccccggg actggtgtca gctgttgcct
     4981 cagtccggta aaacaataac cggcagtaca aacaatcaca tactgtatct gcccggaaaa
     5041 gaaatggaaa aaggcaaatg aaactacgac aatggagata tcacacgaca atttgtagat
     5101 aactcctcat tatacagaaa ccggtgagtt gtattattaa tttttgaatt atgagaaaac
     5161 gaataagtca tttaatttat tttcagtgaa tgtgcaatgt attcgcccat taccggatgt
     5221 tcttcaatca gcgaaaaaca tgcaacaatt ttatatgaac caaactaaac atcaaaaatg
     5281 aagagattat tttttacctg tctgacagcc gctgccatta tcctggcatc atgcggcggc
     5341 ggaacaacgc aaaaagaagg tgcaacgcaa aagacaggca gcacggatga aaagaaggta
     5401 cagacggaag cgaaagccaa ttacctcgac agggaagtgc tggaggccaa actgaaagaa
     5461 atcggcttcc ccatataccc cggggcagaa tttgaaaaga tagaaaaagg aagcggctac
     5521 ttcaagggcg cttatattca gtatgagatt cctgaagtaa gctcagaaag taaggaggcc
     5581 gttgacaagt tctataaaga agtctttgaa aaactggagg catccgggtg gaaaaatgtg
     5641 tttgacgttt actgggaaaa aagagatatg cttttgcatt ttgctcatgt gtacaatgac
     5701 tttcaagctg aaacacattt cctgggtatc actttcgggc tgtccagtga acaataggca
     5761 gtaagcagta acgattattc ctccggcacc tttcaccaca aggcttatta gcgtaaccgg
     5821 gttattttgc agaaggtgaa atttatttgc tttggagcaa tgcctgtatc tgttccaggt
     5881 tgtctttgat gtccatttcc ttcatcatct tcttgctgcc ctccttcatc tcattgaggt
     5941 ttctgagcat attgtcatac accttgctga tagtattttt gtccttcacg gccaatacct
     6001 gttcaatgac cgccctgcgg taagttgaag tattaccaag ctgctccagt gttttcagat
     6061 aatacttctc agttcccgga tcgaactcct gcagtatcaa tccaaactca gagtatggca
     6121 gatttcctac ataaaaacgt actccctttt ccggcttgac tacatcatcc gccggaggct
     6181 gcgagtcgag gcgctttacc tctccgtccc gatcaataaa aaatattcct tcaaattgtt
     6241 gtctgacaga aaccatattg ctgtcctttg aaaccatctc atcccatcct cccaattttg
     6301 gacttatgct gaaacataca ccatccttca ggggtataat ctctgccttc cagcttgaaa
     6361 gataattggg cagattgccg taatcgaaca gacgggtcag gcgaatgccg gtagtatcaa
     6421 aggaatagag ggacacatta ttatggagaa atctggtctt tccttcaatc ggcattataa
     6481 accacaggtt cttgcctttt tgtgaggcac gggtctggca gaggaaatag atctctgacg
     6541 aattgggtga acagactgca ccggaatagg tggcagacag gtcgatatca tgaccataat
     6601 aacctgaaca ggaagcggat atcagcatta taactgcaat atgtaagaag aagacggctt
     6661 tcatatcgta tattgttaaa attcttgccg ggccttgccc gctaatcatt agtcagtcca
     6721 agttacgaaa atctgcggat cagatcctgg cgctgccaga aaaatctctt taaacagcgg
     6781 gatgacagaa atgaatagca gacagtaacg cccatccgaa agagccgtgt aacacagaca
     6841 acacggatac gaaacagggc ggtttctgaa aatttccgtt attacatctg ccacaggtcg
     6901 caattttttc gtaaatttca cacatgtaaa tagttatctt ccggcagtgc tgatgtcatt
     6961 tgtgtcagct ttcattattt gctgcatatt gcttattacg agcaggctgg caggttgtgt
     7021 ccggtcaagg gcagatgtat gtttcgttag aataattatc agcctgaaac cggtgataaa
     7081 acttcataat gaaacagcga cggaaatgac atgtggtgcg ggtgatattg cgaagactgc
     7141 agagctgccg gaatattatc aaactgacaa accatgacaa tgagaaaaat tgcgattatg
     7201 ttggcagtga tactgctgac tcctgcctgc gggaagaaaa cacctcagcc aggcaatgaa
     7261 gaccttgaag gaatatggga atgcatcaaa ggctgcgagg cagaaatgct ggagttcaat
     7321 attgaagatg aaagacaggt attcttcttc tacaccgggc agcgtatgta ccgttccgga
     7381 acgtggaaac tggagaagaa ccggctgaca ctggcctatg atgatgaaga aaccctgaca
     7441 tatccccttg cactgaaaaa cgataccctg gtactgggtg ataataaaat ggtgtttaca
     7501 gcctgcatcc cggctgagga agatccatgc gacctgtctt ccctttttgc actgatggac
     7561 gatatcagtt tcagtcagcc gcttctggag gatttcccct ggaatatccc gggcgattca
     7621 ggtgttgagg aggtgatagt gaacggacag atggttgaaa ccatagtgaa gcttgaagga
     7681 gatttttcag ctatagggag aacggccgga gtcattgctg acgaactcag gaagcttggc
     7741 tatgaagccg atcccctgat ggcctcagaa gttgttaccg gctacaggaa agagaactgt
     7801 gttgttcttt tccggcccgg gattgacgat gaggatgatg ccgatgctga agtgcccttc
     7861 gcggtatatt ttggcaaatt accctgacaa atggtcaaat ccccaattat tgccggccgc
     7921 agtctgatga ttgtcagcag gcaactctta aacccggcct gagacaagct tagcagcgtt
     7981 tggtacctcc ggatgcgtgg cagatcctat cccgtttggt cctgaatcat ggcatatggc
     8041 tctggaaaag tatattattt tacactaccg tccgactaaa ataataagaa aaggggcact
     8101 cccctaacct ctatatatct ggaccccaat gttgtaattt tgttttacta caaaacatac
     8161 tacaacatgg tcaaaattac agaaatgaaa tttttcggac agccgaacct ttcactatcc
     8221 gtctcttcac gatttaaaac tgcgtttcat cacgtccaaa tgactttatg acactgcctg
     8281 gttgtcattc atcacaaata gttgaaaacg cttgtactga ttcttaaatt tgtatttaga
     8341 aacatcattt acaaactgct aatggttctt tgatatactg atccttggat ggaaggttaa
     8401 aaacatttcg taattacccg cgccagcgaa actgcctgca ttaaaacaac aaacagaaag
     8461 attatttagc ggaaatccat tgtctggatg catgtaaaaa ccgcctcccg ggtttacccg
     8521 gatttcagga catgctcagg gaatcgcgga aatgggaatt tggaaatcat ggaaatatta
     8581 aaaacatcaa acgggttttg gattatgaat acttcatttt acaataacat actgacagcc
     8641 ttcattttta tgattatgct ggtcatctca ctgcctgaag ttcaggcgca agactatcgg
     8701 atagcttttg caggttccgg tgcaagcaca actgtcgact ccgtccgtgt tgaaaacctg
     8761 acaacaggaa ccatagtcag acttaatgga accgaccttt tacatcttgg taccgtttcc
     8821 ggtattcagg atttaaacga tacacgaaag gaagaaatta atttctatcc caacccaatg
     8881 cctgagtatt ccgtgatgca tttcaggtta ccggtatccg gttcaacact catagaaatc
     8941 agggatatat caggcaaaac aatctgtcag aaaagggaaa cgctttcaca gggagtccat
     9001 tcatacagga tcagtggaat gggaaaaggg atttacctcg tcagtatcag cagtccggga
     9061 catgtaataa cggggaagct tgtatctgca tgcccaaatt ccgggaagat aaacatcatg
     9121 cgtgaagaga gcattccggc aggaggcaaa accacagatt caaaaggtac cgcagaagag
     9181 gttttcatgc cattcaccgc gggcgacaga ttgaaaataa cagctatctc tgacaagtac
     9241 aggactgtga tagttgactc tcctgaaggg gacaaaacca ttgtattcac ttttattgaa
     9301 tgcactgacg gcgatggaaa taactaccct gtggtagtcc ttgaaaatga agaaggaaaa
     9361 aatgatgggg gaaagtccgg tacaggcaag ggcaaaccgg cattgatggc agaaaatctc
     9421 aaaaccacca aattcaatga tggtaccgat atcccggaga aaagaggctc ggaatgggat
     9481 accccgggtc cggcttattg ctggtataat aataatagat attataatga gaacagatac
     9541 ggggcactgt ataattatgc cgcttttacc tcgggcaaac tatgtcctgc aggatggcat
     9601 gcccctgccg acgaagaatg gaatgatctt tgggactacc ttggtggtgg taatgtcggg
     9661 caaaagctta aggcaagatg ggacacatat tggaatgatg gcgggggaac agacgagata
     9721 ggatttggtg ccaggggcgg tggtatcaga ttaggtaaca acccttacat agatttcgac
     9781 ttacaaaagg aatggtgcta tttttggtgt atcggattta ccttaggtaa cgaagct
//
LOCUS       DAOSPX010000090         9690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6782_length_9690_cov_4.513752, whole genome shotgun sequence.
ACCESSION   DAOSPX010000090 DAOSPX010000000
VERSION     DAOSPX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6782_length_9690_cov_4.513752"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLZ75_06055"
     CDS             <1..919
                     /locus_tag="PLZ75_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HOS71997.1"
                     /translation="AGLAVGHAMIPPTPGPVAVSNILEADLGWVILFGVITGLPAAVI
                     GGVFFGRYISGKIDIPAPEGLEIPGEEPDPATKKVPSYGITALLIFIPLLLILTGTFT
                     KVGVEKNMIPASLLSDIIIFTGHPFTALIIATLLSILLCVRLKMTGQMILDLSTRALG
                     PAGIIILVTGAGGVLKQILVDSGIGVILAESIAGSSIPLVFLAWALAALVRVTQGSAT
                     VAMITAAGIISPVLPLMNLSQPQTAIIVVAIASGATILSHVNDSGFWIVNRYLGMNEK
                     QTLQSWTVMETIISVVGLLLSLLLSVLIR"
     gene            complement(999..2222)
                     /locus_tag="PLZ75_06060"
     CDS             complement(999..2222)
                     /locus_tag="PLZ75_06060"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOS71998.1"
                     /translation="MRIRTKSFLLTTIACGLFFIHGTSPAGDERGNPEQKTAGKYDVA
                     AYVWPSYHPDDRARIFWPAGIGEWETVMKNKPAFEGHEQPRYPLWGYINEADPYVAEM
                     EISAAADHGVNVFIFDWYWYDGMPYLEGHLNNGYLRARNNDRVKFYLMWANHDVDMTW
                     DRRNAGKPNDALIWKGAVDRKEFEIICHRVIGNYFSHPSYYKNDNRPSFMIYDLEALI
                     LGLGGISETKDALEWFRKETRKAGFSGLDLQLSLRRSGSSVRGSSGDILGSQENIIKE
                     LGFDGITHYQFVHFTDIDREYNDIMTDVEKEWKLIDREFKIPYYPHVSIGWDNNPRFE
                     MFRAGIVRNNTPENFKNALIRAKDYADAHPGQVRLITINSWNEWTETSYLQPCTMFGY
                     GYLEAVKKVFVENSR"
     gene            complement(2283..2978)
                     /locus_tag="PLZ75_06065"
     CDS             complement(2283..2978)
                     /locus_tag="PLZ75_06065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOS71999.1"
                     /translation="MPAETGKIKYELVFSRRRSISIIVRPDKSITVRAPFRTPLKDIN
                     RFVLSKSAWIKKHTEGDRRMLLTYHDRTFTDGETFLFLGSEYRLRRIRSETSGVWLDE
                     DEILTGQKDPEDANMTRHLVSGWYAVKAREILPERLAGIIRKYSHAGFRPAGLTVRSM
                     KSRWGSCSSRGRITLNSELVKLKPEIIDYVIIHELCHLKHHNHGPGFYELLSELLPGY
                     KQLRKELRNYRIR"
     gene            complement(2979..4163)
                     /locus_tag="PLZ75_06070"
     CDS             complement(2979..4163)
                     /locus_tag="PLZ75_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS72000.1"
                     /translation="MTFLLFILLLFEILSFLTLKDHFSRGSRSAYYGIMAVHSALSLA
                     VWFYALTVIFFKGHADDPEYINKQLIFSGLLSAILTPRILLCFLHFTGKLLRTKKGGH
                     SRLLTESGLIFSGLIFLVVVHGSYTGRYNFRTEEVTVRLKNLNPSLDGLVIAQLSDLH
                     LSSFFRHPGRLDDVMKRIEKYQPDLIINTGDFVTVGSREFTGFDTILSKYRGRYGNYA
                     ILGNHDMGTYFPSGETEEKRMTPVLMDSLISASGYRVLNDENDIIDIHGVKVAVMGIT
                     TSGRHPRIIHGDIGKAISGTGSAGLKILLCHDPNQWDSEVAGRTDIQLSLAGHTHGMQ
                     IGIITRKYRWSPAAYYYPRWNGLYSEGNQYLYVNRGLGVLGIPFRIGMPPEITIITLR
                     TG"
     gene            4246..4683
                     /locus_tag="PLZ75_06075"
     CDS             4246..4683
                     /locus_tag="PLZ75_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOS72001.1"
                     /translation="MSVVRFYDKSHIPEGKLIYSVIPARYRGKWIFLRKTTRPTWEIC
                     AGHIEDGETSDEAAERELMEETGALEFSLSLIATYSVENEGRTGYGRLYYAEVTDIGD
                     VPDRSEIAERIFADGLPDDLTYPEVQPVLFGRVLNWLGEGHGD"
     gene            complement(4680..5864)
                     /locus_tag="PLZ75_06080"
     CDS             complement(4680..5864)
                     /locus_tag="PLZ75_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOS72002.1"
                     /translation="MKQDFYIEVNSQQRNHDGERICGDVFLYRSIREENRIIAVLSDG
                     MGHGVKANILATLTSTMALNFTREHKEVDRIAEIIMNTLPVCSERKIAYSTFTIVDIE
                     SSGRTNILEYDNPSAIILRGNQEFDPSWKKVILEKGKHEGKVLKTCTLEPEKEDRIIF
                     FTDGVSQSGMGSDDFPFGWERENILSYVSGLVTADASLSANTLAAKIVTMAHKNDGYR
                     ARDDISCSVIYFREPRKLLISTGPPYEKDKDTELASKIKNYQGKVIICGGTTADIVAR
                     ELNRKIVDELIFEDPELPPESFIEEIDLVTEGILTLQKVNEILKTFNNSVHLGKGPAD
                     KIVKLLMESDEIHFIIGTRINIAHQDPSLPVELEIRRTVVKRIARLLEDKWLKKVSFE
                     YI"
     gene            complement(5874..7754)
                     /locus_tag="PLZ75_06085"
     CDS             complement(5874..7754)
                     /locus_tag="PLZ75_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOS72003.1"
                     /translation="MNERKPVLTINEAKCRNSYSCVRVCPVNAIEVRPQKAHPVIVPE
                     RCIGCGLCFVSCARGAVEFRDSKDEVRAILSSGRKTAALIEPSIASEFDDITDYRKFV
                     TMIRSLGFDYVHEVSFGVDLIAAHYVELFSKAEGKYYITANCPAIVKLIEKYHPELTP
                     NLAPLVSPMIATAMVVKELYDEDTALVHIGPCIDAKDEILNYRSGKLIESVLTFIELR
                     QMFDEAGIQERLVKMSEFDPPYGYWGALYPLPPGIIQAGGLKRDMVTSKVITASGSED
                     CLEAIHDFDRHIDTIHHHFNLFFCHGCLLGPGMENHSEKFRRRALVSRYAEKRISTLD
                     KDLWQKNMDRWMNLDFSRTFSPNDQRIPEPPEEAINEVMKIIGKYDAEEKLDCGACGY
                     GSCRDFAIIVAKGLAIPEMCHTFNLRNKQEYIETLRQTNRKLAETKKALKESEELARR
                     EKEIAQGASDMLHNMLEKLTSGVVIVDNNLKILHSNRSFINILGEDAEAISEIIPGLT
                     GADLRTLIPFNVYNMFTYVIREDVPVVSRDVHFDDKMLNISIFPIKKNKMCGAIIRDL
                     YSPEVQGEEVITRVSEVIEKNLEMVQKIGFLLGEGASDTEQMLNSIIESYKKRSNIKN
                     KT"
     gene            complement(7773..8027)
                     /locus_tag="PLZ75_06090"
     CDS             complement(7773..8027)
                     /locus_tag="PLZ75_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOS72004.1"
                     /translation="MISVTEMQICLGSSCFSRGNKDVAVFIKEYLKKHHLEDKVVFRG
                     ARCMGNCTNGPNLSINGKIIEGINLAGIESILDRELAKLK"
     gene            8204..8650
                     /locus_tag="PLZ75_06095"
     CDS             8204..8650
                     /locus_tag="PLZ75_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOS72005.1"
                     /translation="MKKYRPGRREDLIPLLQEIQDENGYLSEDIIVKAGNFIGLSTTK
                     IYGLATFYDQFRFLPAGRIIIRICNGTSCFLNGSQNVISKIREETGLAPGQTSRDGLF
                     SYEIVTCMGGCTRGPLLTVNDDYQLNVKPGQIPDLLRKLRYIAEND"
     gene            8651..>9690
                     /locus_tag="PLZ75_06100"
     CDS             8651..>9690
                     /locus_tag="PLZ75_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72006.1"
                     /translation="MEDIRKFLTTRVLMLESDTLPPETEKVLGQIRRDKTDRPVIFLS
                     TGTSSMIAGSERTRSALRTYLNDSETEVEIIKVGCTGPANFEPLMAIQLPGKNKLFFR
                     NITEEKVEPLLNGVFHNDINHDDLVGQQGGTGFELWPGIPFLCDHPFFSHQKRIILGN
                     CGCYNPESIEEYIARGGYRSFVKAIRHYTYEEVCDIVERSGLRGRSGGGYPTGLKWKQ
                     ALNTSSTSRYLICNAKESDPGAFTDRLIMESDPHRLVEGVAIAAYAIGSSNGIVFYRR
                     GSEHGRQRLQKAIDDAREYGLLGHNIFSSGYNLDIVIRQEPGAFVCGEETALISTLEG
                     KRGMPQLKPPYPT"
BASE COUNT         2344 a         2301 c         2286 g         2759 t
ORIGIN      
        1 ggcaggtctt gccgtgggac atgccatgat ccctcccaca ccgggcccgg tagcggtatc
       61 aaacatactg gaagcagatc tcggatgggt aatccttttc ggagtgatca ccggtctgcc
      121 tgccgccgtc ataggtggtg tgtttttcgg caggtatatt tccgggaaaa tagacattcc
      181 tgcaccggaa ggtcttgaga tacccgggga ggaacctgat ccggctacaa agaaggtacc
      241 ttcatacggc ataactgcat tactgatttt cataccgctt cttctcatcc tcacgggaac
      301 ttttacaaaa gtaggagtgg aaaagaatat gatacccgca tcactcctga gtgatattat
      361 tatatttacc ggacatccct tcacggcact tatcattgcc accctgcttt ccattctcct
      421 ctgtgtaaga ttgaaaatga ccggtcagat gatactcgac ctgagtacca gggcactcgg
      481 gcctgcagga ataatcatac tggttaccgg agcaggggga gtcctgaaac aaatacttgt
      541 tgacagcggc ataggagtga tccttgcaga atcaatagca ggatcgtcaa tacctcttgt
      601 gtttctggcc tgggcccttg ccgccctggt aagggtgaca cagggatctg caacggtagc
      661 catgataacc gctgccggaa taatctcgcc ggtattgccg ctcatgaatc tcagtcagcc
      721 ccaaacggct ataattgtgg tggcaatagc atccggagcc acaattcttt cacacgtcaa
      781 cgacagcgga ttctggattg taaacaggta tctcgggatg aatgagaaac aaacacttca
      841 gtcctggacc gttatggaaa caatcatttc agttgtcggt ctgcttctgt ctcttctgct
      901 gagtgttctg atcagataag accggtgcgg actcattgaa gatcaggata ttccgaattc
      961 aggcccacag gaaagcggca tgatcatttt attttttatt acctgctgtt ttccacaaag
     1021 acttttttaa ctgcctcaag gtatccgtaa ccgaacattg tacatggctg aagatagctt
     1081 gtttcagtcc attcattcca gctgttgatt gttatcaggc gtacctgccc gggatgggca
     1141 tcggcataat cctttgccct gatcagggca tttttaaaat tttccggggt attgttcctg
     1201 actatccctg ccctgaacat ttcaaagcgg ggattgttat cccagccgat tgaaacatgg
     1261 ggataatatg gtattttaaa ttccctgtca atcagcttcc attccttttc cacatcagtc
     1321 ataatgtcat tgtattccct gtcgatgtcg gtgaaatgta caaactggta gtgggtaatc
     1381 ccgtcaaaac caagttcctt tattatgttt tcctgggaac ccagtatgtc cccgctgctt
     1441 cccctcacac tgcttccgct ccggcgaagt gaaagctgca gatccagacc gctgaaacct
     1501 gccttccggg tttctttccg gaaccattca agggcatctt ttgtttcact gattccgccc
     1561 agtcccagga tcagggcttc gaggtcatag atcatgaagc ttggcctgtt atcgtttttg
     1621 taataggaag gatgtgagaa ataatttccg attaccctgt gacatattat ttcgaattcc
     1681 ttcctgtcca cggccccctt ccatattagg gcatcattcg gtttgccggc attgcgcctg
     1741 tcccatgtca tgtcaacatc atgattggcc cacatgaggt agaattttac ccggtcgttg
     1801 ttcctggccc ggaggtagcc gttgttcagg tgtccttcaa ggtatggcat gccgtcgtac
     1861 cagtaccagt cgaagatgaa cacgttaacc ccatggtcgg cagcagcgct gatctccatt
     1921 tccgcaacat aggggtcagc ctcgttgata tatccccaga ggggataacg cggctgttca
     1981 tgtccttcaa aagcgggttt attcttcatg accgtttccc attcccctat gcctgccggc
     2041 cagaaaatcc ttgcacggtc atccgggtga tatgacggcc atacgtaagc cgccacgtca
     2101 tactttccgg ctgttttctg ttctgggttg cccctttcat caccggcagg ggatgtgccg
     2161 tgaataaaaa aaagaccaca ggcgatggta gtcagtaaaa agctttttgt ccggattctc
     2221 atgatggaag tattggttca ggttaactca ttggataaaa ttaaacatta aaaattgtca
     2281 ggctagcgga tacggtaatt tctcagttcc ttccttagct gtttgtatcc gggaagcagt
     2341 tccgacagca gttcatagaa gccgggaccg tggttatggt gtttcagatg gcacagttca
     2401 tgaatgatca cataatcgat tatttccggc tttagtttga ccagttcgga attaagagtg
     2461 atccttccgc gtgaactgca actgccccac cggcttttca ttgaacgtac agtcagtccc
     2521 gccggcctga atcctgcatg actgtatttc ctgatgatcc cggccagtct ctccggcagt
     2581 atttcacgtg ctttcacggc ataccaccct gaaacaagat gccttgtcat attcgcatcc
     2641 tcaggatctt tctgtcctgt caggatttca tcctcatcaa gccacacccc ggatgtttcc
     2701 gagcggatcc tccgcagcct gtactcactg ccgaggaaaa gaaaggtttc accgtcggtg
     2761 aatgtcctgt catggtatgt aagcagcata cgtctgtcac cttcggtgtg cttctttatc
     2821 caggcggatt ttgaaaggac gaaccggtta atgtctttca gtggtgttct gaatggcgct
     2881 cttaccgtta tgcttttatc gggacggaca ataatgctta ttgaccgccg gcgggaaaaa
     2941 accagttcat attttatttt tcctgtttca gctggcattt atccggttct gagagtgata
     3001 atggttattt cgggtggcat tcctatcctg aaagggattc cgagtacacc cagtccgcgg
     3061 ttcacataca gatactgatt tccttcggag tataatccgt tccagcgggg ataataatat
     3121 gctgcagggc tccacctgta ctttctggtt atgattccta tctgcattcc gtgggtatgc
     3181 ccggccagtg aaagctgaat gtctgtcctg ccggccacct cactgtccca ctgattcgga
     3241 tcatggcaca gcagaatctt caggccggca ctaccggttc cggaaatggc tttcccgata
     3301 tccccgtgta ttatccgggg atgccgtccg ctggttgtga ttcccatgac agccacttta
     3361 actccgtgta tatcaattat atcattttca tcattcagta ccctgtatcc cgaagcggat
     3421 atcaggctgt ccatcagtac aggagtcatt cttttttctt cagtttcacc ggaaggaaaa
     3481 tatgttccca tgtcatgatt tccgagtatg gcataatttc cgtatctgcc ccggtatttt
     3541 gacaggatgg tgtcaaagcc ggtgaactcc ctgcttccga cagtaacaaa atcacccgtg
     3601 ttgattatca ggtcgggctg atatttttct attcttttca ttacatcgtc aaggcggccg
     3661 ggatgtctga agaaactgct gaggtgaagg tccgacaatt gtgcaattac cagtccgtca
     3721 agtgaagggt tcagattttt cagcctgacc gtgacttctt ccgtcctgaa attatatctg
     3781 cctgtataag agccatgcac tacaacaaga aatatcagtc cggagaatat aagaccactt
     3841 tccgtcagca ggcggctgtg gcctcctttt ttagttctca ggagttttcc cgtgaaatgg
     3901 agaaagcaaa gcagtatcct gggggtcagt atggcgctta acagtccgga aaaaatgagc
     3961 tgcttgttta tatactccgg atcatcggca tgtcccttga agaaaattac agtcagcgca
     4021 taaaaccaaa ctgcaaggga gagggccgaa tggactgcca tgattccata atatgctgac
     4081 cgtgagcctc gtgaaaaatg atccttcagc gtgaggaatg agagaatctc aaacaggagc
     4141 agtatgaaaa gcaagaatgt catagtcagc aggcagattt caaaaataaa tgaaataaaa
     4201 ttaagacagg aatatttact ttagtctgca taaatttgtg cgattatgtc tgtagtcagg
     4261 ttttatgaca aatcccatat tccggaaggg aagcttatct attcggtaat tcctgcaagg
     4321 tacaggggaa aatggatttt tctccggaag acaacacggc cgacctggga gatatgtgca
     4381 ggtcatatag aagatggtga aacttcagat gaggctgcag aaagggaact gatggaagaa
     4441 accggggcgc ttgaattctc actcagcctg atagccactt attcagttga aaatgaaggc
     4501 aggacaggat atggcaggct ttattatgcc gaagtcactg atatcggtga tgttcctgac
     4561 aggtctgaaa ttgcggaaag gatatttgct gacgggcttc ctgatgatct gacctatcct
     4621 gaggtacagc ctgtgctttt cggccgtgtt ctgaactggc ttggtgaggg gcacggggat
     4681 tagatatatt caaagcttac ctttttcagc cacttatctt caaggagtct tgcgattctt
     4741 tttaccactg tcctgcggat ctcgagctcg acaggcaggc tgggatcctg atgggctata
     4801 tttatcctgg tgcctatgat aaaatgtatt tcatcgcttt ccatcagcag tttcactatt
     4861 ttgtcagccg ggccctttcc gagatgaaca ctgttattga atgttttgag gatttcattt
     4921 accttctgaa gggtaaggat gccttccgtc accaggtcta tttcctcaat gaagctttcg
     4981 ggaggcagtt cgggatcttc aaaaatcagc tcgtcaacaa ttttcctgtt cagttccctt
     5041 gcaacaatgt cggcggtggt tcccccgcag attatgacct ttccttggta gtttttgatt
     5101 ttggaggcga gctccgtatc cttgtccttt tcatagggcg gtcctgtgct gatgagcagt
     5161 ttcctgggct ccctgaagta tatgacagaa cagcttatat cgtctcttgc cctgtagccg
     5221 tcattcttat gagccattgt gactatcttg gctgcaagcg tatttgctga aagggaagca
     5281 tcagccgtta cgagtccgga aacatagctc agtatgtttt ccctttccca tccaaaggga
     5341 aagtcatcgc ttcccatgcc gctttgcgat accccgtcgg tgaaaaatat tatgcggtct
     5401 tccttttctg gttcgagagt gcaggttttc aaaacttttc cctcgtgttt gcccttttcc
     5461 aggatcactt ttttccatga gggatcaaac tcctggttac ccctcaggat tattgcggac
     5521 ggattgtcat attcaagaat atttgtcctg ccgctgcttt ctatatcaac tatggtgaag
     5581 gttgaatagg ctattttcct ctcgctgcat acgggaaggg tgttcatgat tatttccgca
     5641 atcctgtcaa cttccttgtg ttccctggta aagttaagtg ccatggtgga tgtgagagtt
     5701 gccaggatat tggccttgac gccatgtccc atgccgtcgg agagaacagc aatgatccgg
     5761 ttttcctccc ggattgacct gtacaggaac acgtcgccgc atatcctttc cccgtcgtgg
     5821 ttgcgctgct gggaattaac ctctatgtaa aagtcctgct tcaataacgg gctttatgtt
     5881 ttatttttga tattgctcct ctttttgtag gattcaatga tggagttgag catctgttcc
     5941 gtgtccgaag ctccttcccc gaggagaaag ccgatcttct gaaccatttc aagatttttc
     6001 tcaatgactt ccgaaaccct ggttattact tcctcgccct ggacttcggg tgagtacaga
     6061 tccctgatga ttgccccgca catcttgttc ttctttatgg gaaatatgga gatgttcagc
     6121 atcttgtcgt caaagtggac atccctgctt accacgggta catcttccct gatgacatag
     6181 gtgaacatgt tgtacacgtt gaagggaatg agggttctca gatccgctcc ggtcaggccc
     6241 ggaataattt cagaaatggc ttccgcatct tcccccagga tattgataaa gctcctgttt
     6301 gaatgaagga ttttcagatt attgtcaact atcacaacgc ctgaggtaag tttttcaagc
     6361 atgttgtgaa gcatgtcgga ggcgccctgg gcaatttcct tctcccttct ggcaagctcc
     6421 tctgactctt tcagggcctt ttttgtttcc gcaagctttc tgtttgtctg ccggagagtt
     6481 tcgatatact cctgtttatt gcggagattg aaagtatggc acatttcggg tattgccagt
     6541 cctttggcga ctattatggc gaaatcacgg caggaaccgt atccgcatgc cccgcagtcg
     6601 agtttctcct ccgcatcata ctttccgatt atcttcatca cttcattgat tgcctcctcg
     6661 ggaggttcgg gaatcctctg gtcattggga ctgaaggttc ttgagaaatc cagattcatc
     6721 cacctgtcca tgtttttctg ccacagatcc ttgtcgaggg ttgatatacg tttctcggca
     6781 tatctgctta ccagtgccct tctgcggaat ttctcactgt ggttttccat gcccgggccg
     6841 agaaggcagc catggcagaa gaacagattg aaatgatgat gtatggtgtc aatgtgcctg
     6901 tcgaagtcgt gaatggcttc caggcagtct tcactgcctg aggcggttat taccttgctt
     6961 gtaaccatgt cgcgtttcag tccgccggcc tgtattatcc ccggaggaag cggatacagg
     7021 gctccccagt atccgtaagg cggatcgaat tccgacattt tcacaagtct ttcctgtatg
     7081 cctgcctcat cgaacatctg tctgagttca atgaatgtca gcacgctttc aatcagtttc
     7141 cctgaacggt aattcagtat ttcatccttg gcatcgatgc agggtccgat atgaaccagg
     7201 gcagtgtctt catcatagag ctccttcacc accatggccg tggctatcat tggagatacc
     7261 aggggggcga ggttgggagt cagttcaggg tggtattttt caatgagctt gacaatggca
     7321 gggcagtttg cggttatgta gtacttgcct tccgccttgc tgaagagctc aacatagtgg
     7381 gccgctatga ggtcgacgcc gaatgaaact tcgtgaacgt aatcaaatcc gagggagcgg
     7441 atcatggtga caaatttccg gtaatcggtt atatcatcaa attccgatgc tatgcttggt
     7501 tctataaggg cggcagtttt acggcccgat gacaggattg ccctcacttc atcctttgag
     7561 tcgcggaatt ccacagcacc cctggcacag gatacaaagc ataatccgca tcctatacat
     7621 ctttcgggaa caataacagg atgtgccttc tggggcctta cctcgatggc atttaccggg
     7681 cagacacgca cgcaggaata ggaattcctg cactttgcct cgttgattgt caataccggt
     7741 tttctttcat tcattttgct tccttccgga tattatttaa gttttgccag ttccctgtcc
     7801 agtatacttt caatcccggc aagattaatt ccttcaataa tcttaccgtt tatgctcaga
     7861 ttcgggccat tggtacagtt acccatacag cgggcacccc tgaaaaccac tttgtcttca
     7921 aggtgatgct tcttcagata ttcttttata aatactgcaa catccttgtt tcctcttgag
     7981 aaacaggagc ttcccaggca aatctgcatt tctgtaacgc tgatcattac tcttactgcc
     8041 ttttctgtga caacagcctc aaaaataata attattccga ttgcttatta atcccatgct
     8101 atcatattgt taaaaaaata acattgttta ttttgtaaca ataaatattt atatttgtct
     8161 aacctatttc gttatcctga tgagtgcgga aattgaatcc ataatgaaaa aatacagacc
     8221 cgggagaaga gaggatctta ttccgcttct tcaggagatc caggatgaaa atggctatct
     8281 gtcggaagat atcattgtaa aagccggaaa ttttatcgga ctgagcacta caaagatcta
     8341 tgggcttgcc acattctatg accagttcag gttccttccc gcagggcgga taataataag
     8401 aatatgcaac ggaacatcct gtttcctcaa cgggtcgcaa aatgtcatca gcaagatcag
     8461 ggaagagacc ggactggctc ccgggcaaac atccagggac ggcctgttca gctatgagat
     8521 tgttacctgc atgggtggct gtaccagggg acccctgctg accgtgaacg atgactatca
     8581 gctgaatgtg aaacccggac agattccgga tttgctccgc aagctcaggt acattgccga
     8641 aaatgattaa atggaagata tcaggaaatt tctgaccacc cgggttctga tgctggaatc
     8701 ggacactctg cctcctgaaa cggaaaaagt tctcggacag atccgccggg ataaaaccga
     8761 tcgtccggta atttttcttt ctacaggaac cagcagtatg attgccggtt cggaaaggac
     8821 ccgttccgcg ctgaggacat atctaaatga cagtgagaca gaagttgaaa tcataaaagt
     8881 gggttgtacc ggtccggcaa attttgaacc acttatggcc attcagcttc cgggcaagaa
     8941 caagcttttt ttcaggaata ttactgagga gaaggtggaa ccgcttctca acggagtgtt
     9001 tcacaacgac ataaatcatg atgatctggt cggccagcag ggaggcaccg gatttgaact
     9061 atggccggga attccgtttc tttgcgacca tccgttcttt tcacatcaga aaaggattat
     9121 cctgggaaac tgcggctgct ataacccgga aagcattgag gaatatattg caagaggcgg
     9181 atacaggtcc tttgtgaaag ccatcaggca ttatacctat gaagaggtat gcgacatcgt
     9241 tgaaagaagc gggttaaggg gaagaagcgg gggaggttat ccgaccggcc tgaaatggaa
     9301 acaggcgctg aacacttcct caacgtcaag atacctgatc tgcaacgcca aggagagtga
     9361 tcccggggcc ttcaccgaca ggcttataat ggaaagcgac cctcaccggc tggttgaggg
     9421 tgttgccatt gcagcctatg caatcggatc atcaaacgga atagtattct acagaagggg
     9481 ctcggagcac gggcggcaaa gattacagaa ggcaattgac gatgccaggg aatacggcct
     9541 gctcggtcat aatatatttt cgagcggcta taacctggat atagtgattc gtcaggaacc
     9601 cggcgcattt gtgtgcggtg aggaaaccgc tcttataagc actcttgaag ggaaaagggg
     9661 aatgccccag cttaagccgc catatcccac
//
LOCUS       DAOSPX010000091         9631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6854_length_9631_cov_4.536445, whole genome shotgun sequence.
ACCESSION   DAOSPX010000091 DAOSPX010000000
VERSION     DAOSPX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6854_length_9631_cov_4.536445"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLZ75_06105"
     CDS             <1..946
                     /locus_tag="PLZ75_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS72007.1"
                     /translation="ELHRRVVGQDEAIRAVSDAVRRSRAGLQDQKRPVGSFIFIGTTG
                     VGKTELARALAEFLFNDENLMTRIDMSEYQERHSVSRLIGAPPGYVGYDEGGQLTEAV
                     RHKPYSVVLLDEIEKAHPDLFNLLLQVLDDGRLTDNKGRTVNFRNVIIIMTSNIGSDI
                     IRDRMEQYGNNMPEMEEEKLRNEIFGLLRRSIRPEFLNRIDEIVMFHPLNLEQINEIV
                     KIQIGYLRKLLSGNNMDLEITDEAVSWIADHGFDPQSGARPVKRLIQKEIVNELSKQL
                     IAGNIRSDSTVQISVENGKLKFGSLAKKMPVSEEPDRA"
     gene            complement(1143..1481)
                     /locus_tag="PLZ75_06110"
     CDS             complement(1143..1481)
                     /locus_tag="PLZ75_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOS72008.1"
                     /translation="MISTGKQDRIDIININSDRIDALTAENLKREIARVLDKAHPMAV
                     LNLQGVDYIDSTGFGCLLAVHKAAKDNYGVLKFASPEKKVSESLHILNLNTVFEIFES
                     LDDCIRSFNS"
     gene            complement(1482..1844)
                     /locus_tag="PLZ75_06115"
     CDS             complement(1482..1844)
                     /locus_tag="PLZ75_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOS72009.1"
                     /translation="MAYQYINPEYLDSVSAGDTEIVRELVDMFRSQIEETQNEMRTLL
                     DKKDYNSLGLLAHKAKSSVLIMGMADLGSLLKTFEIQAREGIEPHKYESYIDQFSRET
                     SEAVKELEDLVSKRLIRE"
     gene            1997..4123
                     /locus_tag="PLZ75_06120"
     CDS             1997..4123
                     /locus_tag="PLZ75_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOS72010.1"
                     /translation="MRRILFSCFALLLLNILPADAQTNVRIRKKDYRTVREGFKEAWA
                     HVSEGNNYFRERSVWYGSAYEEYRKAMVYNGNNAELNYKAGIAALFSDNKDAAAPFLL
                     KALELKNDISDDILLMTGRALQYSGNYPEAIKMLNDYLNSPVKKTRRNIADASKYLDE
                     CNSALKLVKDTLNVEIRNAGTNINSFADDYSPVFSADFNTLYFASRREVKRSGIPLTD
                     GKFDENIFYSSRIQNEWNVASPLTGSVNTSYCESPLYLNPSGDELYIYTGYMNGGDIK
                     MSKKKNGIWTKPSSIPFSINSRGAETSFTFSPSGDEIWFVTDKGKNSLGGKDIYIIRK
                     KPDSKWSKPENAGPNINTPHDEESVSFSETGDTLWFASKGHNSMGGFDIFYSVRDKNG
                     NWTRAVNCGYPVNTPWNDLFYIPDKSAKNAFYLVSDRKGSLGGLDIFHGRKIAPLPAE
                     EPEPVPVAAPDTIVQKIIVEVQPVRPDTVVVRDTLVVIKEVTAPPPEVPEETFFYLSG
                     TVRDSESGDPVLAKIDVIDISSDMVIATTASSDIDGSYRVRISGKKSYMVDFRGSGFL
                     SDMKRIDIPESFTGDVYKLNMDLVKVKVGRKVVLNNILFQTGKAILTGESYAELNRLY
                     GILEENPHMRIEISGHTDNTGSLALNTKLSQDRARAVVEYLIQKGTDPERLEFRGYGP
                     QQPVDDNTTAEGRARNRRVEFKILEF"
     gene            4127..5227
                     /gene="gcvT"
                     /locus_tag="PLZ75_06125"
     CDS             4127..5227
                     /gene="gcvT"
                     /locus_tag="PLZ75_06125"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOS72011.1"
                     /translation="MKKTPFTAIHQAAGARMVAFAGYNMPMEYSGITDEHLTVREKAG
                     VFDVSHMGEFYVTGPDATDYLQFITSNDITALSDGRIQYSCFPNGRGGIVDDLLVYRF
                     SPEKYLLVVNAANTAKDWDWCVRHAGEFGIVPGRDLVNASDDIAQLAIQGPLAMQIVS
                     RLTEEPVRDMESYTFREMNIAGIKDALLSTTGYTGAGGCEIYVRNEDGLALWNALFEA
                     GKEYGIKPAGLGARDTLRLEMGYCLYGNDIDDETSPIEAGLGWITKFTDSKNFIDREF
                     LLKQKNEGVERRLKGFIMIDRGIPRQHYEIVDDSGNTVGEVTSGTMSPVLKQGIGMGY
                     LEKGYWKPDTEIFIRIRNKDLRAKVFNPPFVK"
     gene            5287..6015
                     /locus_tag="PLZ75_06130"
     CDS             5287..6015
                     /locus_tag="PLZ75_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOS72012.1"
                     /translation="MGKRKLETCFSPLLFEPELHRDSIVVIIDILRATSSICTAFENG
                     VLSMIPVAGVEEAKEYKRRGFLVAAERDGIILDFADLGNSPFNFSRENVEGKTIVYST
                     TNGTGIIKMASSAAATVIGSFLNISSLAGWLLSQDYDVMLFCAGWKNRFNLEDTICAG
                     ALAERLLDSSLYSTICDSTLAALDLWTLAKPDLRGYINKAAHRSRLRKNGLDDCIDFC
                     LSSDFTRKIPVIKNGILTGDIFNL"
     gene            6097..7167
                     /gene="serC"
                     /locus_tag="PLZ75_06135"
     CDS             6097..7167
                     /gene="serC"
                     /locus_tag="PLZ75_06135"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOS72013.1"
                     /translation="MKKHNFYAGPSILPQYTIDKSIEGIRDFAGTGLSVLEISHRSKE
                     FIACMDNAIGLVKELLDVPSGYQVIFLGGGASLQFAMVPMNLMNSRSAYLITGEWASK
                     AFREAKIYGEALEVASSKDKNFSYIPKNYSLPSDVDYFHITTNNTIYGTQLREDPLVP
                     VPLVADMSSDIFSRPVDVSRYSIIYAGAQKNLAPAGVAMVIIKEDVLGKVTRPIPTML
                     DYRTHIKAGSMFNTPPVFTVFAAEQTLKWLKELGGVKAIEKKNIEKAQMLYDEIERNK
                     LFKPTVGDHEDRSLMNICFIMTDEYKELEKDFLEFAKSRGIIGIAGHRSVGGFRASTY
                     NALPAESVKALVDTMKEFEKNR"
     gene            7180..8100
                     /locus_tag="PLZ75_06140"
     CDS             7180..8100
                     /locus_tag="PLZ75_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS72014.1"
                     /translation="MKILIATSKPFAPVAVEQIRKVFETEGYDLALLENYQDTSELLT
                     AVADADALIVRSDLVNASVIDAAKQLRIVVRAGAGYDNIDLNACSERKVVAMNTPGQN
                     ANAVAELAFEMMLYQARGGFAGKTGTELRGKTMGLHGFGNVARCMARIAEGFGMTVSA
                     YDPFISDEVFSEYNVKKCESIEKLYTSSQYVSLHMPANEKTKGSVGYNLLSGMPEDAV
                     LVNTARKEVINEEALLKIFEDRPDFKYLSDVAPDCSSQIAGKYPGRFIFTLKKMGAQT
                     EEANINAGIAAARQIVDYFKTGNEQFRLNK"
     gene            8135..9385
                     /locus_tag="PLZ75_06145"
     CDS             8135..9385
                     /locus_tag="PLZ75_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOS72015.1"
                     /translation="MAIVKPFRGLRPPREIAKDLACLPYDVMSTEEAAAMAEGRECSL
                     LHITRAEIDLPPGTDIHSEEVYGKSVSNFALWQKKGWLRQDEEASYYIYAQTMDGRTQ
                     YGIVGCASVDDYLEGKIKKHELTRPDKEQDRMIHVRVNNANIEPVFLTHPPVKELNAI
                     VKRITTKEKAEYDFVSEDGFGHHFWVIRDPEITLQIEKIFLEKVPCTYVADGHHRTAA
                     AALVGRERRENNPGHTGNEEYNYFLAVHFPADQLRIIDYNRTIKDLNGLSVREFLAKL
                     ESGFEVVKKGTGIYKPKRLHNFSMYLDGFWYSLTAREGTYDDNDPIGVLDVTILTNQV
                     LAPVLDIQDLRRSKRIDFVGGIRGLEELKKRVDSGEMKVAFALYPVSMKQLINIADSG
                     NIMPPKTTWFEPKLRSGLVIHLLE"
BASE COUNT         2796 a         1983 c         2328 g         2524 t
ORIGIN      
        1 tgaactgcac aggagggttg tgggccagga tgaggcaata cgtgcggttt cggatgctgt
       61 gagacgttcc agggcaggtc ttcaggatca gaaaagacct gtaggatcat ttattttcat
      121 aggtacgacg ggagtcggta agactgaact ggccagggca ctcgccgaat tcttgttcaa
      181 tgatgagaac ctgatgaccc gtatcgacat gtcggaatac caggagaggc attcagtgtc
      241 cagactgatc ggtgcgcccc ccggatatgt gggatatgat gagggaggcc agcttacgga
      301 agctgtaaga cacaaaccct attccgttgt ccttctcgat gaaattgaaa aagcacatcc
      361 cgatttgttc aacctcctgc tgcaggtgct tgatgacgga agactgacag acaacaaggg
      421 aagaacggtc aatttccgga atgttatcat tattatgact tcaaacatcg gatcagacat
      481 tatccgcgac aggatggagc aatacggcaa caacatgccg gaaatggaag aggaaaaact
      541 caggaatgaa attttcggac ttctcagaag atccatcagg cctgaatttc tgaacaggat
      601 tgatgaaata gtcatgtttc atcctcttaa tcttgagcag atcaatgaaa tagttaaaat
      661 acagatcggg tatctgagaa agcttctttc aggaaacaat atggatcttg aaattacaga
      721 tgaggctgtc agctggattg cagaccacgg atttgatccc cagtccggag cccggccggt
      781 aaagcgcctg attcaaaagg agatagtaaa tgagctgtcg aagcagctta ttgcgggaaa
      841 tatcaggagt gattccacgg tacagatttc tgtggaaaac ggaaagttga aatttggatc
      901 cctggccaag aaaatgcctg tttctgaaga gccagataga gcctgaagga atctcaggga
      961 acggcatagc cttccactgg tgtagtgctt tccaggcatc tgccacatta agtattctga
     1021 attcctgccg tggttttact gctttttgac ccctttcatg cactgcacaa gagcattgct
     1081 caggttttaa agcggtctgt aaccctaaat tataccggct gtcagcccaa ttatgccggc
     1141 tgtcagctgt tgaatgaacg gatgcagtcg tcgaggcttt caaatatttc gaatacagta
     1201 ttgagattca gaatgtgcag ggattctgat accttttttt cgggagaggc gaatttgaga
     1261 actccgtaat tgtcttttgc cgccttgtga acggccagga ggcacccgaa tccggtactg
     1321 tcgatataat caactccctg caggttcagg accgccatgg ggtgtgcctt gtcaagaacc
     1381 cttgctatct cccttttcag attttcagct gtaagcgcat caatcctgtc actgttgatg
     1441 tttataatat caatcctgtc ctgttttccg gtgctgatca ttcattccct aattagcctt
     1501 ttactgacta ggtcttcaag ctctttaacg gcttcactgg tttctcggga aaactgatca
     1561 atataggatt catatttgtg gggttcaatc ccctccctgg cctggatttc aaaagttttc
     1621 agaagtgacc cgagatctgc cattcccatt ataagtacgg atgatttggc tttgtgtgcc
     1681 agcaggccga gtgagttata atcttttttg tccagaagag tcctcatttc attctgagtt
     1741 tcttcaatct gggatctgaa catgtcaacc agttccctga cgatttccgt gtctcctgca
     1801 gataccgaat ccaggtattc cggattaatg tattgataag ccataggatc agacaatatt
     1861 attaatttac agatttcgac tcatgcaaat atagtatatt tttctgctaa actgaatgac
     1921 agtctgcaat aatacaggtc aaatttttgt gaacggcaaa taaatactaa ttttagggtg
     1981 tgaaaaactg caatttatga gacgcatttt attttcctgt tttgctctgc tgttactgaa
     2041 tatcctgcct gcagatgcac aaaccaatgt caggatccgg aagaaagatt acaggactgt
     2101 cagagaaggc tttaaggagg catgggctca tgtatccgaa ggcaacaact atttcaggga
     2161 gcgctctgta tggtatggaa gcgcttatga agaatatcgt aaagccatgg tatacaatgg
     2221 caataatgct gaactgaatt acaaggccgg tatcgcagcc cttttttcag acaataagga
     2281 tgccgctgct cctttcctgc tgaaggcact ggaactgaaa aatgatatta gcgatgatat
     2341 tttgctgatg acaggaagag cgcttcagta ttcgggaaac tacccggaag caataaaaat
     2401 gcttaatgat taccttaatt ccccggttaa aaagacccgg cggaatattg cagatgcgtc
     2461 aaaatacctt gatgagtgca attccgcatt gaagctggta aaggacacac tgaatgttga
     2521 aatccggaat gctggaacaa atattaattc atttgcagat gattactcac ctgttttttc
     2581 cgctgatttc aatactcttt attttgccag ccggagagaa gtaaaacgtt ccggaattcc
     2641 actgacagac ggtaaattcg acgagaatat attttattcc tcacgtattc aaaatgaatg
     2701 gaatgttgca tcacccctaa ctggttctgt caacacttcc tattgcgaat caccacttta
     2761 cctgaatcct tcaggggatg aattatatat ttataccgga tacatgaacg gaggtgacat
     2821 aaagatgtca aaaaagaaaa atggtatatg gacaaagccc tcttcgattc ctttcagcat
     2881 aaattcgagg ggagctgaaa cctcatttac attttcccct tcgggtgatg aaatctggtt
     2941 tgtcacggat aagggaaaaa attcccttgg cggaaaagat atctatatca taaggaagaa
     3001 gcccgacagc aaatggtcga agcctgaaaa tgccggtcca aacatcaaca cccctcatga
     3061 cgaggagtct gtcagctttt ctgaaaccgg tgataccttg tggtttgctt caaaaggcca
     3121 taattcaatg ggaggatttg acattttcta ttctgtcagg gataagaatg gcaactggac
     3181 cagggccgtc aattgcggct atcctgtaaa tacaccgtgg aatgaccttt tttatattcc
     3241 ggataaaagt gcaaaaaatg ccttttacct tgtttcagac aggaagggaa gccttggagg
     3301 tctggatatt ttccacgggc ggaaaatagc acccctgccg gccgaagagc ctgaacctgt
     3361 accggtggca gctcccgata cgatagttca gaagattatt gtggaagtcc agccggtcag
     3421 accggataca gtcgttgtca gggacaccct tgtggtgata aaggaagtta ctgctccgcc
     3481 tccagaagta ccggaagaaa catttttcta tcttagcggc acggtaagag attctgaatc
     3541 cggagatccg gttctggcaa aaattgacgt gattgatatt tcatcggata tggtgattgc
     3601 cactacggcc agttcagata tagacggatc ctacagggtc agaatttccg gaaaaaaatc
     3661 ctacatggtg gatttcaggg gtagcggatt cctttcagat atgaaaagaa tagatattcc
     3721 ggaaagtttc accggtgatg tttataaact gaatatggat cttgtgaagg tgaaggtagg
     3781 caggaaggtt gttcttaaca atattctctt tcagacaggt aaggcaattc tgacagggga
     3841 atcatatgcc gaactgaacc gtttgtacgg aatactggag gagaatccgc atatgcgtat
     3901 agagatttcc ggtcataccg acaataccgg aagtcttgcc ctgaacacaa agttatctca
     3961 agacagagcc agggcagttg ttgagtatct gattcagaaa ggcacagatc cggagcggct
     4021 tgaattcagg ggatacggtc cccagcagcc cgttgacgac aacacgactg ccgaaggaag
     4081 agcaaggaac cgaagagtag aatttaaaat actggaattt taagaaatga aaaaaacccc
     4141 ctttaccgcg attcaccagg cagcaggagc cagaatggtt gcctttgccg gttacaacat
     4201 gccgatggaa tattccggca tcacagacga acatttaact gtgagagaga aagccggagt
     4261 ctttgatgtg tcccatatgg gtgagtttta tgtcaccggt ccggatgcga cagattatct
     4321 tcagttcata acttcaaacg acattacggc tctttcggat ggcaggatac agtattcatg
     4381 ctttcccaac ggaagaggag gcattgttga cgatctgctt gtttatcgtt tcagccctga
     4441 gaaatacctg ctggtggtca atgcggccaa taccgcaaaa gactgggact ggtgtgtgag
     4501 acatgcagga gagttcggaa ttgtaccggg acgtgatctg gtgaatgcct cggacgatat
     4561 tgcccagctt gcaatccagg gtccgctggc catgcagatc gttagcaggc taacagaaga
     4621 gccggttagg gatatggagt catatacatt ccgcgaaatg aatattgccg gaataaaaga
     4681 tgctctgtta tcaaccactg gctatacggg tgcgggagga tgcgagatat atgtcaggaa
     4741 tgaagacggc cttgctttgt ggaatgccct gtttgaagcc ggaaaggaat atgggatcaa
     4801 acccgccggc ctcggagcca gggatacttt aaggctggaa atgggatact gcctttacgg
     4861 aaatgatata gacgatgaga cttcaccaat tgaggccgga ctgggctgga taaccaaatt
     4921 cacggattca aagaatttca ttgacaggga atttctgctt aaacagaaaa acgaaggcgt
     4981 ggaaagacgt ctgaagggct tcattatgat tgacagggga atcccgaggc agcattatga
     5041 gattgtggat gattccggaa acacggtggg agaagtcacc tcaggaacca tgtcgcccgt
     5101 gcttaaacag ggaatcggaa tgggctatct tgaaaaggga tactggaagc ctgacactga
     5161 aatatttatt cgtataagaa acaaggatct cagggcaaaa gtgtttaatc cccccttcgt
     5221 gaaataaaaa agccctgttt ttatattgtt tgatccttac tacattagct ataaatcacc
     5281 agaaacatgg ggaaaaggaa acttgaaaca tgtttttctc ctctgttatt tgaacctgaa
     5341 cttcataggg attcgatagt tgtgatcatt gatatcctcc gtgccacttc atccatatgc
     5401 acggcatttg agaacggggt gctttcaatg attccggttg ccggagttga agaagcaaag
     5461 gagtataaaa ggagaggatt cctggttgcc gcagaacgtg acggaataat cctggatttt
     5521 gctgacctcg gtaattcgcc ctttaatttt tcacgtgaaa acgtggaggg aaaaacaatt
     5581 gtttacagta ccacaaacgg aaccggaata ataaaaatgg cctcttcggc agcagccacc
     5641 gtgataggct catttctcaa catctcatcc cttgccggat ggcttctttc ccaggactat
     5701 gatgtaatgc tcttttgtgc cggatggaaa aacagattca acctggagga cactatctgt
     5761 gccggagccc ttgcggaaag gctgcttgac agttccctat attccactat ctgtgattca
     5821 actcttgccg cacttgacct gtggacgctt gcaaagccgg atctgcgcgg atacataaat
     5881 aaggctgcgc atcgcagcag gctgagaaaa aacggactgg atgactgtat tgacttttgt
     5941 ctcagcagtg attttaccag aaagataccg gtgattaaaa atggcatact tacaggtgat
     6001 atttttaatc tctaagtttt actgtcaatc tgttttcttt tacaagcctt tttctaattt
     6061 tacagcttcg aatcagcaaa ttttaaacac atacagatga agaaacacaa cttttatgca
     6121 ggtccttcaa ttctgccgca atacacaatt gataaaagca ttgaaggcat cagggatttt
     6181 gccggtacag gattatcagt tcttgaaatt tcccaccgga gcaaggaatt tatagcgtgc
     6241 atggataacg ccatcggact ggtaaaggaa ctgcttgatg ttccttccgg ctaccaggtg
     6301 attttcctgg gagggggagc aagtctgcag tttgcaatgg tgcccatgaa tcttatgaat
     6361 tcccggtcgg cttatctgat aacgggtgaa tgggcaagca aggcattccg ggaagctaag
     6421 atctatggtg aagcccttga ggttgcatca tcaaaggata aaaacttttc ctatattcct
     6481 aaaaactatt ccctgccctc agatgttgat tattttcata ttacgacaaa taataccatt
     6541 tacggaaccc agctcaggga agatcctttg gttcctgtcc ccctggttgc agatatgtca
     6601 tcagacattt tcagccggcc ggttgatgtt tccaggtatt ccataattta tgcaggtgcc
     6661 cagaagaacc ttgcaccggc gggagttgcc atggtcataa taaaggagga tgttcttggg
     6721 aaagttacgc gacccattcc gaccatgctc gactacagaa cccacatcaa ggccggctca
     6781 atgttcaata cacccccggt ttttacggtt tttgcagccg aacagacact taaatggctg
     6841 aaggagcttg gcggagtgaa ggcaatagaa aagaaaaata tcgaaaaggc acaaatgctc
     6901 tatgatgaaa tagaaaggaa caaactattc aagccgacag taggtgatca tgaggaccgt
     6961 tcccttatga atatctgttt tataatgact gatgagtaca aggagctcga aaaagatttt
     7021 cttgaatttg ccaaatcccg gggaatcatt ggaatagccg gtcaccgttc tgtaggcgga
     7081 ttcagggcct caacatacaa tgcccttccg gcagagagtg ttaaagccct ggttgatacc
     7141 atgaaggaat ttgaaaaaaa ccgctaaata tttctgataa tgaaaatact gattgctaca
     7201 tccaaacctt tcgctcctgt tgccgttgaa cagatcagaa aggtttttga aacggaagga
     7261 tatgatcttg ccctgctgga gaattaccag gatacatccg aacttctcac agctgttgcc
     7321 gatgctgatg cactgattgt ccggagtgat ctggttaacg catccgtgat tgatgccgcg
     7381 aaacaactca ggattgtggt acgcgccggg gcaggatatg acaatattga cctgaatgca
     7441 tgttcggaac ggaaggtagt ggcaatgaac actcccggcc agaatgccaa tgctgttgcc
     7501 gaactggcat tcgaaatgat gctttatcag gcaagagggg gatttgccgg aaaaaccggt
     7561 actgagctga ggggaaaaac aatgggactg catggtttcg ggaacgttgc aaggtgcatg
     7621 gcaaggatag cagaaggatt tggtatgacc gtaagtgcct atgacccctt tatcagcgat
     7681 gaagtgtttt cagaatataa tgtaaaaaaa tgcgagagca ttgaaaaact ttatacctcc
     7741 tcccagtatg tttcactgca tatgccggcc aatgaaaaaa caaaaggatc tgtcggatat
     7801 aacttgctga gcggaatgcc ggaggatgct gtgcttgtca atactgcacg gaaagaggta
     7861 ataaatgagg aagcactttt aaaaatattt gaggacagac ccgattttaa atatctttca
     7921 gatgtagcac ctgattgctc atcccagatt gcaggaaaat atccgggcag attcatcttt
     7981 acgctgaaaa aaatgggtgc acagacagaa gaagccaata taaatgcagg tattgccgcg
     8041 gcaagacaga tagtggatta ttttaaaaca gggaacgaac aattcagact gaacaaataa
     8101 agatttttat actgaattat actgaattat actgatggca atagtaaaac cattcagggg
     8161 gctgaggccg ccccgggaaa ttgcaaagga tctggcgtgc ctgccgtatg atgtgatgag
     8221 tacggaagaa gccgcagcga tggctgaggg gcgtgaatgc tcgctccttc acataacacg
     8281 tgctgaaatt gaccttcctc ccggcaccga catacattcg gaagaggttt acgggaaatc
     8341 tgtaagcaat tttgccctgt ggcaaaagaa ggggtggctc agacaggatg aggaagcttc
     8401 ctattacatc tatgcccaga ccatggatgg gcgtacccag tacggaattg ttggctgtgc
     8461 ttccgtggat gattatcttg aaggaaaaat aaagaaacac gaactcaccc gtcccgacaa
     8521 ggagcaggac agaatgattc acgtccgggt gaacaatgcc aacattgagc cggtttttct
     8581 tacccatccc ccggtaaagg aactgaatgc catcgtaaaa aggataacaa cgaaggagaa
     8641 ggcggaatat gattttgtgt cggaagacgg tttcggtcat catttctggg ttatccgtga
     8701 tcctgaaata acgttgcaga ttgaaaagat ctttctggag aaggttccct gtacctacgt
     8761 tgccgacggc caccacagaa cggctgctgc agcacttgtc ggcagggaga ggagagaaaa
     8821 caatccaggg cataccggca atgaagaata caattatttt cttgcagttc attttccggc
     8881 tgatcagttg aggataattg attacaaccg gacgataaag gatcttaatg gattgtcggt
     8941 cagggagttt cttgcaaaac ttgaaagcgg tttcgaagta gttaaaaaag ggaccggaat
     9001 atacaagcca aaacgcctgc ataatttttc gatgtacctt gatggttttt ggtacagtct
     9061 gacggccagg gaaggaacct atgacgacaa tgacccgata ggtgtgctcg atgtcaccat
     9121 actgacaaac caggttcttg ctccagtcct ggatattcag gatctgagaa ggtctaaacg
     9181 gattgacttt gttggaggta taaggggact ggaggagctg aaaaaaagag ttgacagcgg
     9241 ggaaatgaaa gtggcttttg ctctttatcc cgtatcaatg aagcaattga taaatatagc
     9301 agactcaggc aatataatgc cccccaaaac aacctggttt gagcccaagc ttcgcagcgg
     9361 ccttgtaata catctgcttg aatagctctc cgcagttttg ccttttatta aaagcaaaac
     9421 cccgggatat tcattcagat aaagtaattt tgcttctgat aagcccggcc tgttattatt
     9481 ttagtctgtg attcaaaatg tcatttattt cagataatat cagttcgctg aaacggcaga
     9541 taccgtcata tgtcaagctg gttgccgtat caaaaggccg gcccgtttcc gacattcttg
     9601 aagcctataa cgggggacac agaatttttg g
//
LOCUS       DAOSPX010000092         9571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_6900_length_9571_cov_3.781000, whole genome shotgun sequence.
ACCESSION   DAOSPX010000092 DAOSPX010000000
VERSION     DAOSPX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6900_length_9571_cov_3.781000"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLZ75_06150"
     CDS             <1..277
                     /locus_tag="PLZ75_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72016.1"
                     /translation="KFVSLVNLIAGREVVKELLGYSLNRKNLARELSGIVKGGGRREN
                     MLLQYGIIRDKLGPAGASARIAAAMLEELKGQWQETDKGIDKGFTGS"
     gene            282..1457
                     /locus_tag="PLZ75_06155"
     CDS             282..1457
                     /locus_tag="PLZ75_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOS72017.1"
                     /translation="MKLLFYKYYLLAIVLTCLPAFCDGMPPEARLKVRLLSGFNTDSV
                     SFSVVKGAYGMNCFPSGSALLEKGQSAVLFRSGEKLVIKTGNNPFWIVSDSVMLEGRD
                     GNDSFTLSPGEQGQLTRQYSGELLCKADLGTLLLIGSCDEDTYIAGVVKAEGGSGRHR
                     EYFKVQAVIARTYMYRHLYRHAPDGFSLCDDVHCQAFNGITDDSLIIRAVDETRNLVI
                     TGPDSQPIISAFHSNCGGETLPSEHLWLTAQPYLKKVRDPYCAASPNSEWRKSIPAKE
                     WIEYLGRSGMPARPADPAVLNFNQPVRMISYRAGNFSLPFVQIRSDLGLRSSFFSVRL
                     EGDTVILSGRGYGHGAGLCQEGAMVMASRGFDFRKIIAFYYSGVSVTEIRTVSAGTE"
     gene            1477..2235
                     /gene="gldF"
                     /locus_tag="PLZ75_06160"
     CDS             1477..2235
                     /gene="gldF"
                     /locus_tag="PLZ75_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOS72018.1"
                     /translation="MSLFAMFAIFRKEISGFFSSLTGYVVITVFLLVNALFMWVFPGE
                     WNVLENGYAGLETLFFISPWVFLFLVPAVTMRMIAEEKRLGTIELLYSRPVSEREIIW
                     AKYLASVVLVILALLPCLVFYISVHNLGETPGNLDKGGTAGAFIGLFFLAAIYASAGI
                     LASSLTDNQVIAFILAVLACFIMYAGFDSFAYLPGLKKADEFLAGLGINEHYRSVSRG
                     VLDIRDAVYFIAVVLIFNEASRLVLVSRKWRKKN"
     gene            2239..3954
                     /gene="gldG"
                     /locus_tag="PLZ75_06165"
     CDS             2239..3954
                     /gene="gldG"
                     /locus_tag="PLZ75_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOS72019.1"
                     /translation="MQKAGQNENIRIKEWLQFLIRIAAVIVIALLLSLLRIRIDLTED
                     RRYTLSEPTRRILAGTKNDIFIQVYLDGDMPVPLKRLRRSVKEMLEEFRVASKRKIDY
                     EFINPADARDPRQREAQYASLYNKGLNPVNIQASDSEGGKTQKMIFPGMLINYNEIEV
                     PVNFLKNNPSVPYEQNILHSIEGLEYEMIQTIATVSSDTVYKVAFLEGHNELAEIDVA
                     DITIHLAKFFTIDRGAINGRHGILDNYAAVVIAGPAKEFSESDKLVLDQYIMNGGKVL
                     WLAEQVEVNADSLATGGTIGLYRPLNIEDQLFRYGVRINPEVIQDLDCMVIRVAVSAG
                     EQQQMVPAPWVYYPRLYPSQDHPITKNLNRIKGMFVNTIDTVGLDPAIRKTVLLTSSA
                     QSRTITPPVIIRLKEAEQLPDEQQYSKSHLSAAVLLEGVFPSAFRNRMISGLADGKDF
                     AFRDSSEETRMIVVADGDIIRNEVRRMGNQGTPYPLGQDRLTGEMMGNRDFIINCLNY
                     LVDDHGLMELRSREMKLRLLDRTRIREEKLFWQMVNLVVPVLLVIMSGLAYSYFRKRI
                     YTRQG"
     gene            3951..4925
                     /locus_tag="PLZ75_06170"
     CDS             3951..4925
                     /locus_tag="PLZ75_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72020.1"
                     /translation="MKGNAYLRILLPAGVLVLLILVFFLVRNRSPFGKAETRFSGGAG
                     SEITAFEISSAGRKVLLSYRDGVWKVNQDAEARKSITLFALRVLKEMRIKSPVSAGMF
                     YDQIVAKGIRPVKVKVYEKKRLPRTFYVYRTSSNKYGNIMKAKERSRPFVVHFPGFEG
                     DIGSLFIAEEMFWKPFILFNLLPSEISSVTLDNYADPPASFRISTGGESFRLSDLHTY
                     LTGWDTSRLKRYISYYTMVPFESWAFDMAEDEKALLANQKPAYRITVTTNEGENKGLD
                     LWERVSDGKTDAGRLWGKTDDSEDYMIIRYFDIDPLLRKLSYFYPDRK"
     gene            5193..6323
                     /gene="dnaN"
                     /locus_tag="PLZ75_06175"
     CDS             5193..6323
                     /gene="dnaN"
                     /locus_tag="PLZ75_06175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOS72021.1"
                     /translation="MKFVVSSTELLGHLQAISRVISSKNTLPILDNFLFSLSGNDLEI
                     TASDLESTLITRLKLENTEGDGIIALPARLLLDTLKEFSAQPLTFDINLETMGVVLTS
                     ENGKFTMMGLNGIDFPALPVIREDNKFSFTINAEVMLAGINRTLFATADDELRPVMGG
                     IFIDISTDMLTFVASDAHKLVRYQRTDARADDLASFILPKKPASLLKNILPRESGPFV
                     VEFDNKNAFFNLNDYMVICRLVEGNYPNYSSVIPKNNPRKVTIDRVEFYNSLKRVSVF
                     SNQASNLVKLQLKGNEITVSAQDLDFSISANERIKCQYEGDNVEIGFKSVFLLEILAN
                     ISSQEVMIELADPTRAGLFLPAETENAAEDLLMLLMPMMINA"
     gene            6327..7103
                     /locus_tag="PLZ75_06180"
     CDS             6327..7103
                     /locus_tag="PLZ75_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOS72022.1"
                     /translation="MELNLKRPIVFIDLETTGINVSADRIVEISALKINPNGTEQWMT
                     SLVNPEMPIPPKVTEIHGITDSDVAAAPTFREIAKNLAAFLEGCDLAGFNAMKFDMPL
                     LAEEFLRTNVDFNFRKRKYVDVQIIFHKKEQRTLEAAYKFYCRKELKDAHSASADTQA
                     TYEVLKAQLDTYPDLENDIDKLADFSSYNNNVDFAGRIILDENGTEVFNFGKHKGRMV
                     EEVLREEPSYYSWMMNGDFPLYTKKILTEIKLRTFGNKQV"
     gene            7155..8459
                     /locus_tag="PLZ75_06185"
     CDS             7155..8459
                     /locus_tag="PLZ75_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72023.1"
                     /translation="MKEMKKETRREFIKKSAGAAAVLSMPAIIPAGSFGANGKVSVAV
                     IGLNGRGKSHIQGFMNLPDAEVTCLCDCDLNVLDPAVKEFEARYSKKVNKQQDLRKVF
                     EDKSIDAVGIATPNHWHALATIWACQAGKDVYVEKPGSHNIFEGRKMVEAANKYDRIV
                     QHGVQLRSSVAVKEAVHHLRNGLIGRVYMARGLVFRWRGDIGKKGPGTVPKGLDYDLW
                     TGPAEMKPYSEDWVHYNWHWHWNWGNGDVGNQGIHETDLCMWGLDVGLPEVITAGGGK
                     FVFDDSKETPEVLSSTYHYPKEKKFIEFEVRPWMTNAENGVTVGNIFYGTEGYLVVYN
                     YDRYESFLGRDYKPGPSRREGGDHYKNFIDAVVAHDRKILNAPVETAHLSSAPAHLGN
                     IAYRTGRTLYFNPETEKFVGDADADKYLTRPGRPPFVVPEVV"
     gene            8530..8718
                     /gene="tatA"
                     /locus_tag="PLZ75_06190"
     CDS             8530..8718
                     /gene="tatA"
                     /locus_tag="PLZ75_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOS72024.1"
                     /translation="MGNFGLTEIILILLVVVLLFGGRKIPEMMKGLGQGMKEFKKASM
                     ADDEPEKMHAEDKTETKE"
     gene            complement(8742..>9571)
                     /locus_tag="PLZ75_06195"
     CDS             complement(8742..>9571)
                     /locus_tag="PLZ75_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOS72025.1"
                     /translation="PGPMARTVEDAAAFLGANSGIDPEDLKTLESEGKSHRDDTKFLD
                     ADGLKGKRIGLVKNSGGFSVHVDTLMMRAIRDIKAAGAEVTEVDAPPSGTYGGASFQV
                     MLYEFRDGLNRYFSGLAEGMPVRSLKDLIEFNLKDSVELKYFDQNILIEAEKKGDLNT
                     PEYKQALSAMLRATRENGIDRMMDDNRLDALIAPTGSPAWKTDLILGDYSRGGSSSLA
                     AISGYPAITVPMGFVNELPVGITFFGRAWSEALLIEIAYAYEQATRHRKPPRFRATD"
BASE COUNT         2632 a         2042 c         2421 g         2476 t
ORIGIN      
        1 gaagttcgtg tcacttgtca acctgatagc cggcagggaa gtggtaaagg aactgctcgg
       61 ctattcgctg aacagaaaaa acctggccag ggagctttcc ggaattgtaa aggggggagg
      121 ccggagggaa aacatgctgt tgcagtatgg cattataaga gacaagcttg gtccggcagg
      181 agcatctgca agaattgccg ctgcaatgct tgaggaactg aaaggacaat ggcaggagac
      241 tgataaaggt attgacaagg gatttaccgg gagctgataa tatgaaactg cttttttata
      301 aatattattt actggcaatt gtattgacat gcctgccggc tttttgtgat ggcatgccgc
      361 cggaggcccg gctgaaggtg agactgttaa gcggttttaa tacggattca gtgtcattca
      421 gtgttgtaaa gggggcatac ggcatgaatt gctttccgtc gggctctgcc ttgcttgaaa
      481 agggacaaag cgctgttctg ttcagaagcg gggaaaagct tgtaataaaa accggaaaca
      541 atcctttctg gatagtctct gattccgtga tgctggaagg cagagacggc aatgactcat
      601 tcaccctgtc cccgggtgag caggggcagc ttaccaggca atattcagga gaactcctgt
      661 gcaaagccga tctgggtacc ctgcttttaa tcggcagctg tgatgaggat acgtatatag
      721 ccggtgtggt gaaggctgaa ggaggatcag gaaggcaccg ggaatatttc aaggtacagg
      781 cggtgatagc aaggacatac atgtaccggc atttgtacag gcatgctcct gacgggttca
      841 gtctctgcga cgatgttcat tgtcaggctt tcaacggaat tacagatgac tcactcataa
      901 taagggcggt agatgaaaca cggaaccttg tgatcactgg tcctgattca caacctataa
      961 tttcagcatt tcattccaac tgcggaggag aaacacttcc ttcagaacat ctctggctca
     1021 cggctcagcc ttatcttaaa aaagtcaggg atccctattg tgctgcatcg ccgaattcgg
     1081 aatggaggaa aagtatcccg gcgaaagagt ggattgagta tctgggaaga tcaggcatgc
     1141 cggcccgtcc tgctgatcct gccgtactga attttaatca gccggttcgc atgatcagct
     1201 acagggcagg gaatttttcc cttccctttg tacagatcag aagcgatctc ggactccgct
     1261 catcattttt ttcggtcagg cttgaagggg atacagtcat tctgagcggc aggggttacg
     1321 gacacggagc aggtctctgc caggaaggag caatggtcat ggcttcaaga ggctttgatt
     1381 tcaggaaaat catcgcattc tactacagcg gggtcagtgt gacggaaata cggacagtct
     1441 cagccggcac agaataaaaa atttcattat ttttgtgtgt cactattcgc tatgtttgca
     1501 atattcagaa aggagataag cggttttttc agcagtctga ccggctatgt ggtcattaca
     1561 gtcttcctgc tggtcaatgc ccttttcatg tgggtttttc ccggtgaatg gaatgtactg
     1621 gaaaacggct atgccggact tgaaaccctg ttcttcattt ccccctgggt tttccttttt
     1681 cttgtccctg ccgttaccat gcgcatgatt gccgaggaaa agcggctggg aaccattgag
     1741 cttctgtatt ccagaccggt aagcgaaagg gaaataatat gggccaagta tctggcttcc
     1801 gtggtgctgg taatcctggc gcttctgccc tgcctggtgt tttatatttc agttcataat
     1861 ctgggtgaga ctccgggaaa cctcgacaag ggtggtacgg caggagcctt catcggtttg
     1921 ttttttcttg cagccattta tgcttctgca ggaatccttg catcttccct caccgataac
     1981 caggtgattg cttttattct ggctgtgctg gcatgtttta tcatgtatgc aggctttgat
     2041 tcctttgctt atctgccggg actgaaaaaa gctgatgaat ttcttgccgg tctcgggata
     2101 aatgaacatt acagatcagt gagcagggga gtcctggata tcagggatgc ggtatatttt
     2161 attgctgtag tgcttatttt caacgaagcg tccaggctgg tactcgtatc gcgtaaatgg
     2221 agaaaaaaga actgaaacat gcagaaagcc gggcagaatg aaaatataag gataaaggaa
     2281 tggcttcagt ttctgatcag gattgctgct gttatagtga ttgccctgct tctttcactt
     2341 ctccggataa gaatcgacct gacggaagac agaaggtata ctctttcaga accaacccgc
     2401 cgtatactgg ccggtacgaa gaatgacata ttcatacagg tttatcttga cggtgacatg
     2461 ccggttccgc tcaaacgcct gaggagatct gtgaaggaga tgctggagga gttcagggtt
     2521 gcatccaagc gaaagattga ctatgagttc ataaatcctg ccgatgccag ggatccccgg
     2581 caaagggagg cacaatatgc ttccctgtac aacaagggtt tgaatccggt caatattcag
     2641 gccagcgaca gtgagggagg caagacccag aaaatgatct tccccggaat gctgataaat
     2701 tacaatgaaa tagaagtgcc tgtcaatttt cttaaaaaca atccctcggt cccctacgaa
     2761 cagaatattc tgcattccat tgaaggtctt gaatatgaga tgattcagac aattgcaacc
     2821 gtgagttcgg atacggtgta taaagtcgct tttctcgagg gccataatga actcgcagag
     2881 attgatgttg cggacataac gattcatctg gcaaaattct ttacaataga ccggggtgca
     2941 atcaacggcc gccatggtat tctggacaat tacgccgctg ttgtcattgc aggtcccgca
     3001 aaggagttca gcgagtcaga caagctggta cttgaccagt atattatgaa cggaggcaag
     3061 gtgctgtggc tggcggaaca ggttgaagtg aatgccgaca gcctggcaac aggcgggaca
     3121 ataggcctgt acaggcctct gaatattgag gatcagctat tcaggtacgg agtgcggatt
     3181 aatcccgaag taatacagga tcttgactgc atggttatca gggtggctgt ttcagccggg
     3241 gagcagcaac agatggttcc ggcaccctgg gtatattatc ccagattgta tcccagtcag
     3301 gaccatccca ttacaaaaaa tcttaacagg ataaaaggga tgtttgtcaa caccattgac
     3361 actgtgggac tggacccggc aataaggaaa acggttctgc tcaccagttc ggctcagtcc
     3421 agaaccatta ctccccccgt gataatacgt ctgaaagaag cggaacagct gcctgatgag
     3481 cagcagtaca gcaagtctca tttgtctgca gccgtgcttc ttgaaggtgt ttttccctca
     3541 gctttcagaa acaggatgat ttccggcctt gcggacggga aggattttgc attcagggac
     3601 agtagtgagg aaacaaggat gattgttgtt gccgacgggg atatcatccg caatgaggtc
     3661 aggaggatgg gaaaccaggg gactccatat ccgctgggcc aggacaggct gacaggggaa
     3721 atgatgggca acagggattt tataatcaat tgcctgaatt accttgttga tgatcatggc
     3781 ctgatggagc tgaggtcacg ggaaatgaag ctgaggcttc tcgacaggac aagaatcagg
     3841 gaggaaaagc ttttctggca gatggtcaat cttgttgttc ccgtactgct tgtaataatg
     3901 tcaggactgg cgtacagcta tttcaggaaa aggatttata cacggcaggg atgaaaggca
     3961 atgcatattt gaggattctg ctgcctgccg gggtactggt ccttctgata ctggttttct
     4021 ttcttgtcag aaacagatcg cccttcggca aagcggaaac caggttttca ggcggtgccg
     4081 gcagtgaaat cacagctttt gaaatctcgt cagccggcag gaaagtgctt ctttcataca
     4141 gggacggtgt atggaaagtg aatcaggatg cagaagcccg gaaaagcatc acactctttg
     4201 cactgagggt actgaaggag atgaggatca aatcgcccgt ctcggccgga atgttttacg
     4261 atcagatagt ggcaaagggc atccggcccg taaaggtgaa agtgtatgag aagaaaagac
     4321 ttccgagaac attctatgtt taccggacct catcaaacaa atacgggaat atcatgaagg
     4381 caaaagagcg cagcaggccc tttgtcgttc acttcccggg ctttgaggga gacataggtt
     4441 cattgttcat cgccgaagag atgttctgga agcccttcat ccttttcaat ctccttcctt
     4501 cggaaatatc atccgtgacc cttgataatt atgccgatcc gccggcatcc ttcaggatca
     4561 gcaccggcgg agaatcattc aggctttcgg atttgcacac ttaccttacg gggtgggata
     4621 catcaaggct taaaaggtat atatcctact atacgatggt accttttgag agctgggcct
     4681 ttgatatggc ggaagatgaa aaggccctgc tggcaaatca aaagcccgcc tacaggatca
     4741 ctgtcactac aaatgagggg gaaaacaaag gacttgacct gtgggaaaga gtaagtgacg
     4801 gaaaaaccga tgccggccgc ttgtggggga aaacggatga cagtgaggat tacatgataa
     4861 tccggtattt tgacattgac ccgctgctta gaaaactgag ctatttttat cccgaccgga
     4921 aataacaggg ctgcctgatc agatccggat gatccgccgg tatgtcactt cccttattag
     4981 tcaaatcctt tggtgaacag gaaattgttc atatgattca gatatcagcc ccggacttta
     5041 ttaaacgata agttgtattt aaatttgaat gaatatattt tttttgtaat attgatagct
     5101 gtgaatcagg tcatatctgt tgatctaatt cattaagagt tagttgtata agtaaataca
     5161 aagctgaata aataatttaa acgtataata atatgaaatt tgtagtatcc agcactgaat
     5221 tacttggtca tcttcaagct ataagcaggg ttataagttc aaagaacaca cttcccattc
     5281 tggataattt ccttttcagt ctttcgggaa atgatctgga gataacagct tcagatcttg
     5341 aatcaaccct cataacaaga ttgaagctcg agaataccga aggagacggc attattgccc
     5401 tgccggcaag gttattgctg gataccctga aagagttttc agcccagcct ctcacctttg
     5461 acattaatct ggaaacaatg ggtgttgttc ttacttcaga gaacgggaaa ttcacgatga
     5521 tgggtctgaa tggaattgat ttcccggctc ttcccgtcat cagagaggat aataaattct
     5581 cctttaccat taatgccgaa gtgatgctcg ccggcattaa cagaacgctc ttcgccactg
     5641 cagatgatga actgcgtccg gtaatgggag ggattttcat tgacatatcc acagacatgc
     5701 taaccttcgt tgcctcggat gcgcataaac tggtcaggta ccagagaaca gacgcccggg
     5761 cggacgatct ggcttccttc attcttccca aaaagcccgc ttcactgctg aagaatatcc
     5821 tgccaaggga atcaggaccc tttgttgttg aattcgataa taaaaatgcc ttttttaatc
     5881 tgaatgatta tatggtaatc tgcaggctgg tagaaggcaa ttatcccaat tacagttcgg
     5941 tgattcccaa aaacaatccc aggaaggtaa ccatcgacag ggtggagttc tacaattccc
     6001 ttaaaagagt atcggtattc tccaaccagg cgagcaatct tgtcaaactt cagctgaaag
     6061 gaaatgaaat caccgtttct gcgcaggatc tggatttttc catttctgcc aatgaaagga
     6121 tcaagtgcca gtacgaggga gataatgtgg aaataggatt caaatcggtc ttcctgcttg
     6181 aaatacttgc aaacatctct tcacaggaag tgatgattga acttgccgat ccgacaaggg
     6241 ccggactatt ccttccggca gagactgaaa atgctgcgga agatctcctg atgctgctta
     6301 tgcccatgat gataaatgcc tgataattgg aactcaatct caaacgcccg atagtattta
     6361 ttgatctcga aacaacaggc atcaatgttt cagcagatcg cattgtagag atatctgcac
     6421 tgaaaatcaa ccccaacggt actgaacagt ggatgacaag cctggtcaat cctgaaatgc
     6481 ccatccctcc taaagtaact gaaatacatg gtattaccga cagtgatgtt gccgctgcac
     6541 caacattcag ggaaatagca aaaaatcttg ctgcttttct tgaaggatgc gacctggcag
     6601 gtttcaatgc catgaaattt gatatgccgc ttctggcgga ggaatttcta agaacgaacg
     6661 tggatttcaa tttcaggaaa agaaaatatg ttgatgtcca gattattttt cataaaaagg
     6721 aacaacgtac cctggaggct gcctataaat tctactgcag gaaggaactg aaggatgccc
     6781 acagtgcttc tgccgatacc caggcaacct atgaggtgct gaaagcccag cttgacactt
     6841 atcctgatct tgaaaatgat atagacaagc tggcagattt cagttcctat aataacaatg
     6901 ttgattttgc cggcaggatc attcttgatg aaaacggcac ggaagtgttt aatttcggga
     6961 aacataaggg aaggatggta gaggaagtgc tgagggagga accttcctat tattcctgga
     7021 tgatgaacgg ggattttccc ctgtacacca agaaaatact tacagaaatc aaactcagga
     7081 cattcggaaa caagcaggta taaaagaagt aataattgtt ttcaggatat attttttaac
     7141 ctaatactga catcatgaaa gaaatgaaaa aagaaaccag aagagagttc attaaaaaat
     7201 cggcaggggc agctgcggtt ctttccatgc ctgcaataat tccggcaggc tccttcgggg
     7261 ccaacggaaa agtatcggtg gcagtaatcg gactcaatgg ccgggggaaa agccacattc
     7321 agggttttat gaatcttccg gatgccgaag ttacctgtct ttgcgactgt gacctcaatg
     7381 ttctggatcc ggcggttaag gagtttgaag ccagatattc gaaaaaggtg aataaacagc
     7441 aggatctgag aaaggtattt gaagacaaat ctattgacgc tgtgggtatt gctactccga
     7501 accactggca tgcccttgca accatctggg cctgccaggc aggaaaggac gtttatgtag
     7561 agaaaccggg ctctcacaat atattcgaag gccgcaagat ggttgaagct gcaaacaagt
     7621 atgacagaat agtgcagcat ggcgttcagc tgcgcagttc cgtcgcggta aaggaggctg
     7681 ttcaccatct tcgcaacgga ctgataggaa gggtgtacat ggcaagggga ctggttttcc
     7741 gctggagagg agatatagga aagaagggac ccggcacggt accgaaggga ctcgattatg
     7801 atctctggac cggtcctgca gagatgaaac cctattcgga agactgggtg cattataact
     7861 ggcactggca ctggaactgg ggaaacggcg atgtgggcaa ccagggtatt cacgaaacag
     7921 acctttgcat gtggggcttg gatgtgggac ttcctgaagt gattacagcc ggaggaggga
     7981 aatttgtatt cgatgattca aaagaaacac ctgaagtgct ctcatctaca tatcactatc
     8041 cgaaggaaaa gaaattcatt gagtttgagg tccgtccgtg gatgaccaat gcagagaacg
     8101 gagtaaccgt ggggaatatt ttctatggaa cggaaggtta cctggtagta tataattatg
     8161 accgctatga atccttcctc ggaagagact ataaacccgg tccttcgcgc agggaaggcg
     8221 gagatcatta caaaaatttc attgatgccg tagttgctca tgacaggaaa atactgaacg
     8281 cccctgttga aacggcacat ctttcatcgg ctcctgcaca tcttggcaat atagcataca
     8341 ggaccgggcg gaccctttac tttaatcctg aaactgaaaa atttgtcggg gatgcggatg
     8401 ccgataaata tcttaccagg ccgggcaggc cgccttttgt ggttccggaa gtggtttagc
     8461 cggcatagtg agatttgtta atcagtttta cttatactat ttttacaggg taatttaaac
     8521 tcttgaagaa tgggaaattt tggattaact gagatcattc tcattttgct ggttgtcgtc
     8581 cttcttttcg gaggaaggaa aatacctgaa atgatgaagg gactcggaca gggaatgaag
     8641 gaattcaaga aggcatccat ggctgatgac gagcctgaaa aaatgcatgc tgaggacaag
     8701 actgaaacaa aggaatagaa accggcatat ctccgggcag ttcagtccgt cgccctgaag
     8761 cgtggcggtt tcctgtgcct ggttgcctgt tcataagcat aggctatttc gataagcaga
     8821 gcttcgctcc atgccctgcc gaagaaagtt attccaacgg gcagttcgtt tacgaatccc
     8881 atgggaactg ttatggcagg atatcctgaa attgctgcaa gggatgaact gcctcccctg
     8941 gaataatccc ccagaatgag atcggttttc catgctggtg atccggtggg tgctatcagt
     9001 gcatccagcc ggttatcatc catcatcctg tcaatgccgt tttcgcgggt agccctgagc
     9061 atggctgaaa gtgcctgctt atattccggg gtattcagat cacctttttt ctcagcttca
     9121 ataagaatat tctggtcaaa atatttcagt tcaaccgaat ccttcagatt gaattcaatc
     9181 aggtctttca gggatctgac gggcatgcct tcagccagcc cggagaaata tcggttcaat
     9241 ccgtccctga actcatagag catcacctgg aatgaagctc cgccatatgt tcctgacgga
     9301 ggggcatcta cttcagtgac ctctgcccct gcagctttta tatcacgtat ggctctcatc
     9361 atgagggtgt ccacatgaac ggaaaaacct cctgagtttt ttaccagacc gatccgtttt
     9421 cctttgagac cgtcagcatc aagaaatttt gtatcatccc ggtgggactt cccctctgat
     9481 tccagagttt tcagatcttc cggatcgatc ccggaatttg cccccaggaa agcagctgca
     9541 tcttcaactg tcctggccat ggggcccgga g
//
LOCUS       DAOSPX010000093         9410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7035_length_9410_cov_5.052165, whole genome shotgun sequence.
ACCESSION   DAOSPX010000093 DAOSPX010000000
VERSION     DAOSPX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7035_length_9410_cov_5.052165"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2827)
                     /locus_tag="PLZ75_06200"
     CDS             complement(<1..2827)
                     /locus_tag="PLZ75_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOS72026.1"
                     /translation="MNSGTLTIYSASAGSGKTFQLTRIYLTHIFRSRYNYRKILAVTF
                     TNKATAEMKNRILDQLYRLANGEQSEYLDDLVKDTGLSAEKIRSEAAGLLSAILHDFS
                     RFSISTIDSFFQKVIRSFARESGLHSGYNVELDHSIILSAAIDEIIASSASDRELKDW
                     LTEYVMKNLEEEKSWNLKGEITRLAEELFREKFKTLPEDERKKLEDKNYLRAYIEKIK
                     SIITSIDKDVRDFGKKLQDYYTRFGLTDDMFYQKSRGVPGFIKSMREGKIILPNNYVR
                     QILGDNPKWSSKDPAPGLLNAIREGLHETLTGAIEYCERNYQVYRTAMIVSDNIYTLG
                     ILSDVLIQVRKAATDENSFLISDTAELLSLITEGDQTPFIYEKIGNAFENFMIDEFQD
                     TSFLQWKNFQPLISESMGRGCDNLIVGDIKQSIYRWRNSDWQILADLQATKADNERIL
                     KRSLENNWRSRSGIIKFNNTLFSIIPRQIDMAFSEEGLESDLENLYEGAIQSDPGRKS
                     GGYIRLEFSEVKDDAADNSAKSDTRKSLKAWKTRISEKIPEIIELFQDHGYRAGDIGI
                     IVREGREGEEVVRKIIEYSNNCPPEKKEKYNYNVVSDDSLALSSSWAITFIIAVLKVL
                     HDPGDVISRAEMLRFYMLLHRRDKAAEFSLYREMLMDETADLFPEGCDRFLAGIRNRP
                     LFDVTENIISFFGLGDYAENVPYLQTFQDLVLNFSRNRTSDTDTFLDWWENGGSRKAI
                     SLPSGQNSIRVLTIHKSKGLEFPVVILPFLAWNIDHPAGKQPVLWIRPGIAPFNEVEM
                     IPVKYGKALSETLFADDYAREKYYSCIDNINLLYVAMTRARDAVYGFIPLSEGGKMSV
                     SSILRNAIDSDDNPAGNRGFVPGKYFNAADGVFEYGTIPRIDEIREVSKTETTESYRV
                     NIKPESLRLRLHGIDYFTPSGEDKMRRI"
     gene            3112..3366
                     /locus_tag="PLZ75_06205"
     CDS             3112..3366
                     /locus_tag="PLZ75_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72027.1"
                     /translation="MNDSEKAGDNEKEDNSERVNDSEKEGNIERAGDGERPDGSERED
                     NSERVNDGERACDSEWVSKSVSKKGNKDKNENMTFSIFAG"
     gene            3363..4562
                     /gene="mnmA"
                     /locus_tag="PLZ75_06210"
     CDS             3363..4562
                     /gene="mnmA"
                     /locus_tag="PLZ75_06210"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOS72028.1"
                     /translation="MKKKVVIGLSGGVDSSVAACLLRDQGYDVTGLFMRNWNDTTGLL
                     QSDCHWEDDLMFAEMVAKKAGIPFHMVDLSEAYKKRVVDYMFSEYGKGRTPNPDILCN
                     REIKFDSFAEEAKKLGADLIATGHYCRKDFISTDTGTIHRLLRGKDENKDQSYFLCQI
                     SQNQLAEALFPLGNLTKEEVRALAADLRLPTASRKDSQGICFIGKVHLPVFLQQKLKS
                     SSGPVIEIGGNAAFSSWPAGLPDDTITDDELKNMSSPHQPGNLAGKNIGTHHGAHFFT
                     IGQRKGINIGGYREPLFVIGTDTDNNILYVGEGQSHPGLYRKGLFIRAGEIHWIRNDR
                     AMNPGETRDCLVRIRYRQPLQQARLFRRKDGMYIIFNRLQRSITPGQFAAWYEGDEVT
                     GSGVIDK"
     gene            4590..5360
                     /locus_tag="PLZ75_06215"
     CDS             4590..5360
                     /locus_tag="PLZ75_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS72029.1"
                     /translation="MMKKISVVSGGSGGLGLAIAEQLAGKGKNVLLLGRNASKLEEAK
                     RKVEKAARKKGEAGRKPTEAGKKPDPVPDNSIIRTLVCNVGNETDIRNTGQFLRDNNL
                     ITEYLFNNAGKGHFAPATEADSVIIDDILEANLKGLILLSSEILRITPEEEELTIINI
                     MSTSALLGRDQETIYCAAKWGARGYTEALRAELKGSKRNIIAVYPGGMKTGFYEAAGV
                     KRNLEGFMNPEEVAEKIVSSVLHSDRLTVSDITINRKK"
     gene            complement(5381..9382)
                     /locus_tag="PLZ75_06220"
     CDS             complement(5381..9382)
                     /locus_tag="PLZ75_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS72030.1"
                     /translation="MRGSGTTTAALTDNIISGSSNSGIKVQDRTVNIIGNALNQCGTG
                     VSLDNAVATIYDNSITNSTSYPLIQLNNALPTYLNNTITGSGPSAIGIGGTISANRTL
                     SNLQNMGLVYAVISDIIIASGATLTIEAGTIIKFEYRTTTTRLTIIVNGRINPQGTAS
                     NNVIFTSLRDDSVGGDDNMDGSATRPAPGDWGCIQINSTNNNLSHCTFRYGGYHYHSQ
                     SGSRIWLWINNSTNPAIEISDCTFEYAFGTAIYYDDIMHNATPVITNNIINNCPVGIH
                     LRGSGTTTATLANNRITNSVSNGIYLHNIGEGTNIRKNTISDNGTGIYVNSGAPNINE
                     NNISNNSSYGVNNNGSSEVNATGNYWGDSSGPYHSTLNPDGKGNQVSDKVNFSQWTME
                     TIVTLESVTLTFLSPARSFSAPALKTNAKSFIYFYYDTNSSTAKPGSSFHYSLTYNGK
                     SIPSSVKILDAGIVELCVDLGQISQTGSLLISIPESITSRDKILNIQNRPTDFRKGIQ
                     MREIEQNIDLFAEASLGGSFIAGGVGAGPSIAAARFSLSGSGGVGITFKRDIHKDQYV
                     SRRFEASITGSVSSPAINAVAGKIQLGLDANITQSLFLGQTMLFPEELSNDIRKKAKA
                     AYILETLILANVARSPVTALVLESLHKDDPNVGASYNDLIYSFETGLGLEGDASIGFS
                     VAWGSENDQSKLDILDVGAKFGLNNQISIYPSKNEYSFCLDFAREFSLSMLNLQLGAI
                     DVGTLFRLGEYNNGTNFSLDANFSTSDGFSSLVVSTNSSSSKEVSLRNYTTHKSIHYI
                     VPKSVISRNINRNDLLNAISEKWNTGRPIKAVKVGLDYFINNVRSLYDSSSDSLVSYH
                     DHIMIENNNKRLKGNTFNIGIDLDAALGIGVGLELGLMCSYFDELHYPESESVLAHGK
                     AFPLFEYNGISNDTNLFNIKDELKDLLSGTIGLIKAQLKQLVNKIEYYVDKGIHFIIG
                     DDDLSEYKILGKLYEAGKLIIRVINPAGRVMLKEMFLESQVKTAYISDRIIDKNAKGL
                     NDFGINSRVYFVSQVVNVNFIDNTERVVPSFDPLTLSLGIDPAKMDELGFGENEKKLA
                     KLYLYDPENIAWIELAGNTSKHADTVTTLITKSGSYAVGIEINPEHDIFPPQITDYFP
                     RDNDSTNAYPIYWAKLIEGPTEVGVDLSRTSLFIDGREVSASWDPTNKIISYSQTDSL
                     SLGLHTFNVVATDYNGNQQEISSTFRVVLATTAIEKTMINNPDFEFYPNPTSDFVTIS
                     FDALSPGKICLDVYNQGGQKVATLINDSGFSSERKFIWDLTDNNGKRLASGIYFLRMR
                     NNERIIVKKLIIH"
BASE COUNT         2748 a         1971 c         2030 g         2661 t
ORIGIN      
        1 ctatccttcg catcttgtcc tcacctgatg gtgtgaaata atcaatacca tgaagtctca
       61 gcctgagtga ttccggtttt atgttcaccc tgtagctttc cgtcgtctcg gttttactga
      121 cttccctgat ttcatcaatc cggggtatag tgccgtactc aaacactccg tccgcagcat
      181 tgaaatattt tcccgggaca aaacctctgt tccccgcagg attgtcatca gaatctatgg
      241 catttctcag aatggaggaa accgacattt tcccgccttc gctcaacggt atgaagccat
      301 aaacagcatc cctggccctt gtcatggcta catacagcag attaatattg tcaatacagg
      361 agtaatattt ttccctggca taatcatcag cgaaaagagt ctccgacaaa gctttgccat
      421 acttaacagg aatcatttcc acttcgttga aaggagctat cccgggtctt atccacagaa
      481 ccggctgctt gcctgccggg tggtcaatgt tccatgcgag aaacggaaga atcaccaccg
      541 gaaattcaag tcccttggac ttatggatag tcaataccct tatgctgttc tgaccggacg
      601 gaagggatat tgcctttctg ctcccgccgt tttcccacca gtcaagaaaa gtatccgtat
      661 cggaagtcct gttgcgggaa aaattcagaa ccagatcctg gaatgtctgc aggtagggta
      721 cgttttcagc ataatcacca agaccgaaga atgagattat gttttccgtc acatcaaaca
      781 gggggcggtt ccgtatcccg gcaagaaaac ggtcacatcc ttcgggaaac aggtctgctg
      841 tttcatccat aagcatttcc ctgtacaaac tgaattcagc tgccttgtcc ctgcggtgca
      901 acagcatgta gaagcggagc atttccgccc tgctgataac atcccccgga tcatgcagca
      961 ctttcagaac tgcaataatg aatgttatgg cccaggaaga tgacagtgca agagaatcgt
     1021 cagagacaac attgtaatta tatttctctt ttttctccgg cggacagtta ttactgtatt
     1081 ctattatctt cctgacaacc tcctctcctt ccctgccttc ccggactata atacctatat
     1141 ccccggcccg gtaaccatga tcctggaata attcaataat ctccggtatt ttttcggata
     1201 ttcttgtttt ccaggccttt aatgatttcc ttgtgtcact cttagcggaa ttatctgcag
     1261 catcatcttt tacctcactg aattcaagtc ttatatagcc accgcttttt ctgcccggat
     1321 cagactgaat cgctccttca taaagatttt ccagatccga ttccaggcct tcctctgaaa
     1381 aggccatgtc aatttgccgg ggaataatcg agaacagagt gttgttgaat tttatgatcc
     1441 cggaacggct tctccagtta ttttccagtg accttttcag gatcctttca ttgtcagcct
     1501 tcgtggcctg cagatcggca agtatctgcc agtcactgtt gcgccagcgg taaatggatt
     1561 gcttgatgtc acccactatg agattatcgc accccctgcc catactctcg gatatcagcg
     1621 gctgaaaatt tttccattgc agaaatgaag tgtcctgaaa ctcgtcaatc atgaaatttt
     1681 caaaagcgtt ccctatcttt tcgtagatga aaggtgtctg gtcaccttcc gtgatcaggc
     1741 tcagaagctc agccgtatcg gatatcagaa aactgttttc atccgtagct gccttacgga
     1801 cctgaatcag tacatcagaa aggattccga gggtatagat attatcggaa acaatcattg
     1861 cagtcctgta aacctgataa ttcctttcac aatattcaat tgccccggtc agggtttcat
     1921 gcagtccttc cctgatggcg tttaacagac cgggggcagg gtcttttgac gaccatttgg
     1981 gattatcacc caggatctgc cttacataat tattcggcag gatgattttt ccttcccgca
     2041 ttgattttat gaaaccgggg actcctcttg atttctgata gaacatatca tcggtaagtc
     2101 cgaaacgggt ataataatcc tgaagctttt tcccgaaatc cctgacatct ttgtcaatgg
     2161 aggttataat gctttttatt ttctctatat aagcgcgcag ataatttttg tcttccagct
     2221 ttttcctctc atcttccggc agtgttttaa acttttccct gaaaagctcc tctgcaagcc
     2281 gggtaatctc ccctttcagg ttccacgact tttcctcctc aaggtttttc attacatatt
     2341 ccgtcagcca gtctttcagt tcacggtcgg atgccgaaga agctattatt tcatcaatcg
     2401 ccgcagacaa aataatacta tggtccagct caacattata cccggaatga agtccgcttt
     2461 cccgggcaaa ggaccggatc accttctgga aaaaagagtc gatggtggaa atcgaaaacc
     2521 tggagaaatc atgaagaatg gcagaaagca ggccggctgc ttcgcttctg attttttcag
     2581 cactcaggcc tgtgtccttt acgagatcat caagatattc ggactgttcg ccatttgcca
     2641 gacggtaaag ctgatcaaga atcctgttct tcatttcggc tgttgcctta ttggtaaagg
     2701 tcaccgcaag tatcttccgg taattgtacc tcgacctgaa gatatgggtg agatagatgc
     2761 gggtgagctg aaatgtcttt cccgatcccg ctgaagcgct gtatatggtt agtgtaccgg
     2821 aattcacgaa ataaaggtac tgatttttat tttgagcatg gaacaggagg aataatatta
     2881 accgtgtcag tgaagcaacg gaaatagaat tagaaataga attagaaata aaaaaagaaa
     2941 gagaacgaga gtgagggtga gatagttaga atgagaatga gaatgagaaa gagaatgaga
     3001 gagtgagaga gagtgagaga gagtgagagt tagtgaaaga gaggacaata gtgagaattt
     3061 gagcgatagg agaaagttgg cgatgttgag agagtgtgcg atagtgagag ggtgaacgat
     3121 agtgagaaag cgggcgataa tgaaaaagag gacaatagtg agagggtaaa cgatagtgag
     3181 aaagagggga atattgagag agcgggcgat ggtgagagac cggacggtag tgaaagagag
     3241 gacaatagtg agagggtgaa cgatggggag agagcgtgcg atagtgagtg ggtgagcaag
     3301 agtgttagca agaaaggaaa taaggataaa aatgagaata tgacttttag tatctttgcg
     3361 ggatgaagaa aaaagtggtc ataggattgt cgggaggggt tgattcgagt gttgccgcct
     3421 gccttctaag ggatcagggc tatgatgtga ccgggctatt catgaggaac tggaatgaca
     3481 ccaccggact gctgcagagt gactgtcact gggaagacga tctgatgttc gcggaaatgg
     3541 tggcaaaaaa ggccggaata ccttttcata tggtagacct tagtgaggca tataaaaaga
     3601 gagtggtaga ctacatgttt tcagaatacg ggaaagggag aactcccaat cccgacatcc
     3661 tgtgcaacag ggaaataaag ttcgattcct tcgcggagga agcaaaaaag cttggtgctg
     3721 accttattgc aacaggacat tactgccgga aagatttcat cagtacggat accggtacca
     3781 ttcacaggct gcttagagga aaggatgaaa acaaggacca gagctatttt ctatgtcaga
     3841 taagtcagaa tcagcttgct gaagcattgt ttccactggg gaacctcacc aaggaggagg
     3901 taagggcttt ggctgcagat ctcagactgc ccactgccag ccgtaaggat tcgcagggaa
     3961 tatgctttat aggaaaggta catctcccgg tttttcttca gcaaaaactt aaatcatcca
     4021 gcggaccggt aattgaaatc ggtggaaatg ctgctttttc atcatggcct gcaggtcttc
     4081 cggatgatac gataacggat gatgaactga agaatatgtc ttctccccat cagcccggga
     4141 atctggcggg aaaaaacata ggcacacacc atggtgcaca tttttttacc ataggccaga
     4201 gaaagggtat aaatatagga ggctacaggg agccgctctt tgttatcgga actgataccg
     4261 ataacaatat tctctatgtg ggtgagggac aatcgcaccc cggcctttat cgtaaagggc
     4321 tgtttataag ggccggtgaa attcactgga tcaggaatga ccgtgccatg aatcccggcg
     4381 agacacggga ttgcctggtg agaatccgtt atcgtcagcc tctccagcag gcacggcttt
     4441 tcaggagaaa agatggcatg tatataattt tcaacagatt gcagcgcagc ataactccgg
     4501 gacagtttgc agcctggtac gaaggcgatg aagtgaccgg atcaggagtg attgacaaat
     4561 aattgtcagg gtcaacaaaa caataaatta tgatgaaaaa aatatcagtt gtaagcggag
     4621 gatcaggagg tctcggattg gcaattgcag aacagcttgc agggaagggt aaaaatgttc
     4681 tccttcttgg caggaatgcc agtaagctgg aggaagcaaa aaggaaagtg gagaaagcgg
     4741 caaggaaaaa gggggaagct ggaagaaaac cgacggaagc agggaaaaaa ccggatcctg
     4801 ttccggataa ctccatcatc cggacactgg tctgcaatgt ggggaacgaa accgatatca
     4861 gaaatacagg tcagttcctc cgggataaca atctgattac cgaatatctt ttcaacaatg
     4921 cgggtaaggg acattttgcc ccagccacgg aagcagactc agtaatcatt gatgatattc
     4981 ttgaagccaa tctcaagggc ctcattctgc tcagctcgga aatactgaga ataacacctg
     5041 aggaagaaga acttaccatt ataaatatca tgtctacctc tgcacttctg ggacgcgatc
     5101 aggaaacaat ctactgtgct gcgaaatggg gagccagagg atacaccgaa gcactccggg
     5161 cagaactgaa gggaagcaaa agaaatatta tcgcggttta tcccggagga atgaaaaccg
     5221 gattttatga agctgccggt gtgaagcgca atcttgaagg cttcatgaac ccggaggaag
     5281 ttgcggaaaa aatagtcagt tctgtcctgc atagtgacag actcaccgtg agtgatatta
     5341 ccataaatag aaaaaagtaa caggagaatg actattacag ttaatgtatt atcagctttt
     5401 taacaattat cctttcattg ttcctcatac ggaggaaata aataccactt gcaagtcttt
     5461 tgccattatt gtcagtcaga tcccaaataa attttctttc gctggaaaat cctgaatcat
     5521 tgattaaggt tgcgactttc tgaccaccct ggttgtatac atcgagacat attttacctg
     5581 gagataaagc atcaaaagaa atagttacaa aatcagaagt cgggttagga tagaattcaa
     5641 aatcaggatt attaatcatt gttttctcaa tagctgttgt agccaaaaca accctgaatg
     5701 ttgaacttat ttcctgttga ttaccattgt aatcagttgc aaccacatta aaagtatgta
     5761 atccaagtga caaggagtct gtctgcgaat aagagattat tttatttgtt ggatcccagc
     5821 tggcagacac ctctcgtcca tctataaaca gagatgttct tgacaggtca actcctactt
     5881 ctgttggtcc ctcaatcagt ttcgcccaat aaattggata ggcattggtt gagtcattat
     5941 ctctggggaa ataatcggta atctgtggcg ggaaaatgtc atgctcagga tttatttcaa
     6001 tacctacggc atatgaaccg gatttagtga tcaatgtagt tacagtatct gcatgtttac
     6061 ttgtattacc cgccagctca atccaggcaa tattttcagg atcatatagg tatagtttag
     6121 ccagtttttt ttcattctca ccaaaaccca gctcatccat tttggccggg tctataccca
     6181 atgacaatgt aagcggatca aaagaaggaa ctaccctctc agtattatcg ataaaattaa
     6241 cgttaacgac ctgactgaca aaatagactc tactgttaat tccaaagtca ttaagtccct
     6301 tcgcattttt atctataata cggtcggaaa tgtaagcagt ttttacctga gactccaaaa
     6361 acatctcctt tagcataact ctgcctgccg gattaatcac tctgattatt agtttacccg
     6421 cttcatacag ttttcctaag atcttatatt ccgacagatc atcatcccct attatgaaat
     6481 gtattccttt gtctacataa tactctattt tgtttaccag ctgttttagt tgtgctttga
     6541 tcaaacctat tgtgcctgag agcagatctt ttaattcatc tttaatgttg aaaagattag
     6601 tatcatttga gattccgtta tactcaaata atggaaacgc ctttccatgg gctaacaccg
     6661 attcactttc cggataatgt aattcatcaa aatatgaaca cattaaacct aactctaatc
     6721 caacgccaat gcctaaagct gcatcaaggt caataccgat attaaacgta tttcctttca
     6781 atcttttgtt attattctca atcataatat gatcatgata tgaaaccagg gaatcagagg
     6841 aggaatcata taaagatcta acattattaa tgaaatagtc aagtcctact ttgacagctt
     6901 ttattggtct gcccgtgttc cacttctcag aaattgcatt taataaatca tttctattaa
     6961 tattcctgct gatcacagat ttgggaacga tataatgtat tgatttatga gtagtgtaat
     7021 ttcgtagtga aacttctttg gaagaagatg aattggtaga aaccacaaga gaactaaaac
     7081 catctgatgt tgaaaaattt gcatccaatg aaaagtttgt accattattg tattctccca
     7141 gcctgaataa tgttccgaca tcaatagcac ctaattgtaa attaagcatg gagagagaga
     7201 actccctggc aaaatcgagg cagaatgaat actcattttt tgaaggataa atgcttattt
     7261 gattattaag accaaattta gctccgacat caagaatatc aagtttactt tgatcgttct
     7321 cgctgcccca ggctactgaa aaacctatag aggcatcacc ctccagacca agtcctgttt
     7381 caaacgaata aattaaatca ttgtaagatg caccaacatt tggatcatct ttatgcagtg
     7441 actccagtac taatgctgta actggacttc ttgcaacatt cgccagaatc aaagtttcaa
     7501 gaatgtatgc ggcttttgct ttttttcgta tatcattgct taactcctca ggaaacaaca
     7561 ttgtttgccc cagaaacaag ctttgagtaa tattggcatc tagtcctaat tgtatcttcc
     7621 ctgcaaccgc attaatcgcg ggcgatgaga cggatccagt tatactggct tcaaatcgtc
     7681 tgctaacata ttgatcttta tggatatcgc gtttaaaagt aatgccaacg ccgcctgagc
     7741 cgcttaagga gaaacgcgca gctgcaattg acggccccgc accaactccc cctgcaataa
     7801 atgatcctcc cagactcgct tcggcaaaga ggtcaatgtt ttgttcgatt tcgcgcatct
     7861 gaatcccttt tctgaaatcc gttggcctgt tttgtatgtt aagtatttta tcacgggagg
     7921 taatagattc cggtattgag atcaaaagac ttccagtttg gctgatttga cctaaatcaa
     7981 cacaaagctc cactataccc gcgtcaagga tcttgacgga agagggtatg gacttgccat
     8041 tataggttag actataatga aaagatgaac ctggcttagc agtgctggaa ttagtgtcat
     8101 aatagaaata gataaaggat tttgcatttg tttttaatgc aggtgcggaa aagcttctgg
     8161 cgggtgataa aaaagtcaat gttacggatt ctaaagttac gattgtttcc attgtccact
     8221 gcgagaaatt tactttgtcg ctcacctggt ttcctttacc atcaggatta agagttgaat
     8281 ggtatgggcc tgaagaatca ccccaataat tccctgtggc gtttacttcg gatgatccgt
     8341 tattatttac tccgtaactt gaattatttg aaatattatt ttcattaata ttcggggctc
     8401 ctgaattcac ataaatacca gttccattat cgctgatcgt atttttccgg atattggttc
     8461 cctcaccgat attatgtaaa taaattccgt tggaaacgct atttgtaatt ctattattcg
     8521 ccagtgttgc agtagtcgta ccactgcctc tcaaatgaat accaacaggg caattgttga
     8581 tgatattgtt tgtaatgaca ggagttgcat tatgcattat gtcatcataa tatatagcag
     8641 tcccgaacgc gtattcaaaa gtgcaatccg aaatctcaat tgcaggattg gtagaattat
     8701 ttatccataa ccaaattcga gaacccgact gactatgata atgatagcca ccataccgaa
     8761 aagtacaatg cgacaagtta ttattggtac tattgatctg tatgcacccc caatcccccg
     8821 gagcaggccg cgtcgcactg ccatccatat tgtcatcccc accaacggaa tcgtccctga
     8881 gtgaagtaaa tattacatta ttagaagctg tgccctgtgg atttatcctt ccgttgacaa
     8941 taattgtaag cctggtagta gtagtcctgt attcaaactt aatgatcgtg cctgcttcaa
     9001 tggtaagagt tgcacctgac gcaataataa tgtcgctaat tacagcatac accaacccca
     9061 tgttttgtaa attgctcaat gttctattgg cagatatcgt tccgccaatc ccaatcgcac
     9121 ttggcccgct tccggtaatt gtattattta aatacgtggg caatgcatta ttgagctgta
     9181 ttaatggata acttgttgaa ttggttatcg agttatcata aatagtagca acagcgttgt
     9241 caagagatac gcccgtacca cactggttta atgcattccc gatgatattg acagtcctgt
     9301 cttgaacctt gatcccacta ttgctggaac cggaaataat attgtctgtt agcgctgcag
     9361 tagtcgtacc actgcctctc aaatgaatac caacagggca attgttgatg
//
LOCUS       DAOSPX010000094         9390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7054_length_9390_cov_4.683985, whole genome shotgun sequence.
ACCESSION   DAOSPX010000094 DAOSPX010000000
VERSION     DAOSPX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7054_length_9390_cov_4.683985"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLZ75_06225"
     CDS             <1..724
                     /locus_tag="PLZ75_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HOS72031.1"
                     /translation="GLYGLLSTMNLMGRSTTLNIYAPSKYREILFSHLLDFDIILGFN
                     IDFVALDSGDPVKILDDKHLTVTSFPLRHRIPSYGFLFREKAADRNIVKEAIEKFSIP
                     VARIPAIKKGEDFILENGEVIRNDEITVAGPVPSSYAYCSDTMYFKRLSSFVRNVDLL
                     YHEATFEAGLGELARITGHSTSTDAARTAFEAGAGTLIIGHFSSRHKDVQPLVREARS
                     IFPRTIAAVDGTTYDVGNIDVP"
     gene            933..2534
                     /locus_tag="PLZ75_06230"
     CDS             933..2534
                     /locus_tag="PLZ75_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOS72032.1"
                     /translation="MKRTGKGRALLLGLFFLAVQNVPAQILSEEIIKIGKTLNLIEEY
                     YVDTANINKITENAIITMLRELDPHSTYISAKDVREMNEPLLGNFDGIGIQFNILRDT
                     IIVIEPISGGPSEKAGLLAGDRIVSINGEEVAGKGMSTNGVRSRLMGPKGTKVSINVF
                     RRGEKNILDFTIIRDKIPINSLDAAYMLDSETGYVRFNKFAATTDKEFRDALSRLRKA
                     NMRNIIIDLRGNGGGFMAASTQLAGHFFPDGKLLVYLIGRKTPRQEYKSSGSGDLTSA
                     RVVILTDDGSASASEIVAGAIQDWDRGIIIGRRTFGKGLVQNAFTLTDGSMIRLSIAR
                     YYTPAGRSIQSPYNEGYEKYLENYTRRFTNGEMMSADSVHFPDSLKFRTLVNQRVVYG
                     GGGIMPDVFVAVDTSNYSDYYRTLVRRGIFNSFILEYADKNRAAITSRFKTFENFKNA
                     FGFSPDEIQAFVKKGEDAGVNYVEDQFRKSEKEILLVLKALLANNIWQTNEYFRIINE
                     QDNIIAEALKVISDEKLYNRILGNK"
     gene            complement(2559..2990)
                     /locus_tag="PLZ75_06235"
     CDS             complement(2559..2990)
                     /locus_tag="PLZ75_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72033.1"
                     /translation="MKMRKKTGLTGVLIITLLLSMASCKKDKALSEIILGKWEVISLT
                     MVTYENNVKRAELIVYFNPGEMHYQFIDGGVGILYEEENDHLFSWTLSGGKIIISKLY
                     TEDLVAEITIEDSNLSWTYRKNDDETPGISYEFIMTAKRIK"
     gene            3157..4308
                     /locus_tag="PLZ75_06240"
     CDS             3157..4308
                     /locus_tag="PLZ75_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOS72034.1"
                     /translation="MKLMKLIAKTLFGLEAVLAAELADLGAAGIETMNRAVSFTGSIE
                     TMYRVNYCSRTALSVLMPVGEFMISSGEDLYRNALKINWPEFMDSDSAFSVVPVVNSK
                     LFRHSAYPGLLLKDAVADYFRNKSGRRPSVNTADPVVLINLHISNDRVTVSLDSSGYP
                     LYKRGYRSGQGPAPLNEILAAGIILMSGWNGLDPLLDPMCGSGTIPVEAGLIACKIPP
                     GKFRKKFGFTGWKNYDAELFGRIRHEAGAAVRKPDTLIRAEDVSAKAIAICRANIENA
                     GLSGLIQTEVADFRNAKPGFSRGFIFMNPPYGKRLHAQGDDNLYSLIGTTLKHNYSGN
                     KAWVIVPDRESLNSIGLKPGEKHKLFNGAIECILAAYELYEGSGKRRTG"
     gene            4420..4974
                     /locus_tag="PLZ75_06245"
     CDS             4420..4974
                     /locus_tag="PLZ75_06245"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOS72035.1"
                     /translation="MSFNIETYLRNHPESNVILYYKGNVDSDLINYVLDTVEEKLAKM
                     DEQPRLRKKVYNVLVESLQNLYHHVDKVPEDFEDQHSEKFGILRIEKIPTGYKIVTGN
                     FIQTDNAKELKEKIMRINRSTREEIKELYKFILNHQRLSAKGGGGLGLVDIARKTGNK
                     LDYTFRRYNDDYFFFYLDILVAVE"
     gene            5010..5390
                     /locus_tag="PLZ75_06250"
     CDS             5010..5390
                     /locus_tag="PLZ75_06250"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOS72036.1"
                     /translation="MEPIKIEGTPKTPTVKFDKSEGVFEIKGRSIPENSVEFYKPLVD
                     WLDNYKEDPLPKTEVNIRLEYFNTSSSKCILDVFKKLEAIHKAKNEVVVNWYYEEDDE
                     DMLEAGEDYESIIRVPFKMIEITE"
     gene            complement(5467..8541)
                     /locus_tag="PLZ75_06255"
     CDS             complement(5467..8541)
                     /locus_tag="PLZ75_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS72037.1"
                     /translation="MSEKILEILMQLFAIIAKPQADDTERREVVESFLYRLISQDLVK
                     KYLSRYDEAYEEARKRLERVSSKRREGAIAVRIWRLCVEINEQGRLDHEQRIFVVIQA
                     LEFCRSGNRTVSAMELGFIRTLSEGLNIKKEEFDLIELFILTPIVKIPDSQNLLLIDG
                     NPSSSLRETRHIHRELLKGQIWILFIPSVNMYFLRYRGSGELSMSVQLLQEDKVYPFS
                     EGSSLRFSKVKPVYYWDVTMQFLMEDFQSSRVVYEVNNLQYRFRSGEIGLHHMSFKTE
                     SGRMVGIMGASGAGKSTLLHVLNGSDQPSDGEVLINGINIHREREKIKGLIGFVSQDD
                     LLIEDLTVFQNLYYNARLCFGNLSNVEIIEKVDNVLKNLGLYDIRNMKVGSPLNKKIS
                     GGQRKRLNISLELIREPSILFLDEPTSGLSSRDSENILDLLKDLTRKGKLLFIVIHQP
                     SSDIFKMFDKLIILDTGGYPVYNGNPVESIDYFKKKIEQTNYNESECYVCGNVNPDQI
                     FNILETRVYSESGQATDTRRISPADWYEFYLADKKEEELERGNSIPDINFNIPNKVRQ
                     MIVFVKRDILSKVSDIQYLLITLLEAPVLALFLAYLIRYFDESSAVPRYILYENANLP
                     VYIFMSVIVAIFMGLTVSAEEIIKDRKILKREAFLNLSWNSYLMSKVIVQFSISAIQA
                     FSFVLIGNWITGIRGMLFEYWLVLFSCWAGANMLGLVISDSFKAVVTIYILIPFLVIP
                     QIMLSGVMVKFEKLNPQMSSPVSIPFYGEMITARWGYEALAVKQFKDNLYGRQFYVYD
                     KAMSQAKYRKDYLHPEIKGRLERIQSDLERGTLNEESFRKLKLVYNEFTKQAVLTPQL
                     KFRHLEHLTPEGVTPEIVREALDYTEEVRRYYVACSNLAKDKKDAIVTRLQAADSKAF
                     LAMRDNYANEALEEFVINKNETEKIIVYRDEIIQKLDPVYMDPEHNFIKAHFYAPVKK
                     LFGRETDTYWVNVIVLWCITLMIYSVLYFRILKKILDSGNKAMERKNSSSGP"
     gene            8774..>9390
                     /locus_tag="PLZ75_06260"
     CDS             8774..>9390
                     /locus_tag="PLZ75_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOS72038.1"
                     /translation="MNLNNFTLKAQEAVQKAFNITRGKGQQAVECAHLLKGVISEAES
                     ISDYLFGKAGIDTRLLIRDIDRLIDSYPKVSGAEAYISPNLSEAFRKAGDHASDMKDK
                     FITPEHLILGVMGTGDESAGILRDHGATMKGLVSAISELRKGAVVNSQTSDDTFNSLE
                     RYALNLNERARSGKLDPVVGRDDEIRRILQILERRTKNNPLMIGEP"
BASE COUNT         2682 a         1974 c         2117 g         2617 t
ORIGIN      
        1 cgggctttac gggcttctgt ccactatgaa cctgatggga agaagcacaa cactaaacat
       61 ttatgctccc tcaaaataca gggaaatatt gttttcacac cttctggact tcgatataat
      121 acttggtttc aatattgatt tcgttgctct ggacagcggg gatccggtaa aaatactgga
      181 tgacaaacat cttaccgtaa catcctttcc tctcagacac aggatcccgt cgtatggttt
      241 tttgttcagg gaaaaggcag ctgaccggaa tattgtaaag gaggcaattg agaaatttag
      301 tatcccggta gcaagaatcc cggcaataaa gaagggtgag gattttatcc tggaaaacgg
      361 ggaggtaatc agaaatgatg aaataaccgt agccggacct gttccgtcgt cttatgccta
      421 ttgcagtgat acaatgtatt ttaaaagact ttcttccttc gtcagaaatg ttgatcttct
      481 gtatcatgag gctacttttg aagccggcct gggagaactg gcccggataa ccgggcattc
      541 aacaagtact gatgcggcca gaacagcctt tgaggcagga gccgggacac tcataatagg
      601 gcatttctcc tcacggcata aagatgtaca gccccttgtc agagaggcca ggagtatatt
      661 tccgagaaca attgccgccg ttgacggaac aacatatgat gtgggaaata ttgatgtccc
      721 gtaaggaaag aatttctatc tttgtcatct ccctgactaa aatgttcagg ttgcttgttt
      781 tcccggaacc gacgacacag acaaaagcag tatttagaaa tggcaatact gactaaagtg
      841 cctgaagtaa aaggagttga tgtaatcaat gggatcagag gggagatggt ttgatttaaa
      901 atgatttata atttcaggta atagtttgtt aaatgaaaag gacaggaaaa ggaagagcat
      961 tgttgttggg attgttcttc ctggcagttc agaatgttcc ggcacaaata ctttccgagg
     1021 aaattatcaa gataggaaag acactgaatc tgatagagga gtattatgtt gacactgcca
     1081 atatcaacaa aataacggaa aatgccatca tcacaatgct gagggaactt gaccctcatt
     1141 ccacctatat ttccgctaag gatgtgaggg aaatgaacga acctctgctt ggaaatttcg
     1201 acggaatagg aattcagttc aatattctga gagacacaat tatagttatt gagccaattt
     1261 caggaggtcc ttccgaaaag gcaggactcc ttgccggaga caggattgtg agcataaacg
     1321 gggaagaagt tgcaggaaaa ggaatgtcga ccaacggggt cagaagccgc ctgatggggc
     1381 caaaaggaac gaaagtcagc ataaacgttt tccgcagggg cgaaaaaaac atactggatt
     1441 tcactattat acgtgataaa attccgatta acagcctgga tgccgcctac atgctcgact
     1501 ccgaaacagg atatgtaagg tttaataaat ttgcggccac aactgacaag gagttcaggg
     1561 atgctttaag cagactcaga aaggccaata tgaggaatat cattattgac ctgcgcggga
     1621 atggtggcgg gtttatggct gcttccaccc agctggccgg tcatttcttc ccggatggca
     1681 aactgctggt ttaccttatc ggaaggaaaa ccccacggca ggaatataaa tcttcaggat
     1741 ccggtgatct cacctcggca agggttgtta ttctgactga tgacggatcg gcatcggcaa
     1801 gcgaaatcgt ggcgggagca attcaggact gggacagggg catcatcatc ggaagaagaa
     1861 catttggaaa gggattggtg cagaatgcct ttaccctgac ggacggatcc atgataaggc
     1921 tttcaatagc acgctactat acacccgcgg gcagatctat tcagagcccc tacaatgagg
     1981 gatatgaaaa atacctggaa aattataccc gcagatttac aaacggggaa atgatgtcgg
     2041 ccgacagtgt ccactttccc gattcactta aattcagaac gcttgtcaat cagcgtgttg
     2101 tttacggagg agggggaata atgcccgatg tattcgttgc cgtggacact tcaaactatt
     2161 ccgattatta caggacactg gtgagaaggg gcattttcaa ttcatttatt cttgaatatg
     2221 ccgataagaa cagggcagca ataacttcaa ggttcaaaac atttgagaac tttaaaaacg
     2281 ctttcggttt ttctccggat gaaatacagg cttttgtgaa aaaaggcgaa gatgccggag
     2341 tgaattatgt tgaagatcag ttcagaaaat ctgaaaaaga aatcctcctg gtgcttaagg
     2401 cattgctggc caataatata tggcagacaa acgaatattt ccggataatc aatgaacagg
     2461 ataatataat tgctgaagca ctgaaggtta tttctgatga gaagctttat aaccggatac
     2521 tgggaaacaa gtaacagtat cctgatgcct atcaggaatt attttatcct tttagcggtc
     2581 attatgaatt catagcttat tccaggtgtt tcatcgtcgt ttttcctgta tgtccagctc
     2641 agattactgt cttcaatcgt gatttccgcc accagatcct ccgtatagag cttgcttata
     2701 ataatcttgc ctcctgacag tgtccaggaa aagagatggt cgttctcctc ttcatataga
     2761 atgccaaccc ccccgtcaat gaactggtaa tgcatttctc ccgggttgaa gtaaactatt
     2821 aattcggctc tttttacatt attttcatat gtaaccatgg taagggaaat gacttcccat
     2881 tttcccaata tgatttcgct cagggcttta tcctttttgc aggatgccat cgacagcaag
     2941 agagtaatta tcaatactcc tgtcaacccc gtcttttttc gcattttcat taatttgaat
     3001 tttatgtggt aaaactattt caacatattt ttggaaagga caaaaataag gaaactattt
     3061 ttattgcggg ataaaagaga ttggcatata tattgcaaat tgcaaatgtt tatttttgcc
     3121 ggatttattt aaccgaattt accggatgga acataaatga aacttatgaa gttgattgcc
     3181 aaaacccttt ttggtctgga ggctgtcctg gctgctgaac ttgcagatct cggagctgcc
     3241 ggtattgaaa ccatgaaccg tgcagtatca ttcaccggca gtattgaaac catgtaccgg
     3301 gtcaattatt gttcccgcac agccctgtct gttcttatgc cggtcgggga attcatgata
     3361 tcatccggag aagaccttta caggaatgcc ttgaaaatca actggccgga atttatggac
     3421 agtgattctg ctttttcagt tgttccggtt gtaaactcaa aattattcag acacagtgcc
     3481 tatcccggcc ttttgctgaa ggatgcggtt gctgattatt tcaggaacaa atccggcagg
     3541 aggccttcgg ttaatactgc cgatccggtg gttctcatca atcttcatat cagtaacgac
     3601 agggtgactg tttccctgga ttcatcgggt tatcccctgt ataaacgtgg ttaccggtcg
     3661 ggtcagggac ctgcacccct gaacgagatc cttgctgccg gcataatatt gatgtcgggc
     3721 tggaacggtc tcgaccctct gctcgatccc atgtgcgggt caggtaccat tccggttgag
     3781 gcaggactga tcgcctgcaa aataccaccg ggtaagttca gaaagaaatt tggttttacc
     3841 ggatggaaaa attatgatgc tgaactgttc ggcaggataa ggcatgaggc cggagcagct
     3901 gtcaggaagc cggatacact gatcagggcg gaggatgttt cagcaaaagc aattgccata
     3961 tgcagggcca atattgaaaa tgccggcctt tccggactga ttcagactga agtggcggat
     4021 ttcagaaatg caaaacccgg tttcagccgg ggcttcattt ttatgaatcc gccctacggg
     4081 aaaagattgc atgcccaggg tgatgacaat ctgtacagtc tgataggaac taccctgaaa
     4141 cataattatt ccggaaataa ggcatgggtt attgttcctg acagggaaag cctgaacagc
     4201 atcggcctga aacccggaga gaagcacaaa ctttttaacg gggctattga atgtatactt
     4261 gcagcatatg agctgtatga gggttcaggt aaaagacgga ccggataaaa tcattgttaa
     4321 tttcaaaacg tgtaatgtga acaagttatc ctgtggatgc tttttatttt tattatattt
     4381 gtcagggttt aaattccaac cttttcaaag gaagcttata tgagctttaa tattgagact
     4441 tacctgagaa accatccgga aagtaatgtt atactttact ataagggaaa cgttgattcc
     4501 gatctgatca attatgttct tgatacagtt gaggagaaac tggctaaaat ggatgagcag
     4561 ccaagactca ggaagaaggt ttacaatgtt cttgtagaga gccttcagaa tctctaccat
     4621 catgtggaca aagttcctga agattttgaa gatcagcatt ccgagaaatt cggcattctc
     4681 cgtatagaaa aaattcccac gggctacaag attgtgacag gtaattttat acagacagac
     4741 aatgcgaagg agctgaaaga aaaaataatg aggataaacc gctccacccg ggaagaaatc
     4801 aaggaacttt acaaattcat tcttaatcac cagaggttat cggcaaaagg gggaggaggg
     4861 ctcggactgg ttgacatagc caggaaaaca ggaaataagc ttgattatac attcaggcgc
     4921 tacaatgatg attatttctt tttttacctc gatatacttg tagctgttga atagaaaata
     4981 caattaataa aatcataaat tttagtgaaa tggaaccgat aaaaatagaa ggaacaccga
     5041 aaacaccaac tgtgaaattt gacaaatcag aaggcgtatt tgaaattaaa ggtcgttcaa
     5101 ttcctgaaaa ctcggtagaa ttctacaaac cgctcgttga ctggcttgac aattacaagg
     5161 aggatcccct cccgaaaacg gaagtaaata ttcgcctgga gtattttaat acaagttcct
     5221 caaaatgcat tctggatgtg tttaaaaagc tggaggccat tcacaaggca aagaatgagg
     5281 tggtggtaaa ctggtattat gaggaagatg atgaagatat gctcgaagcc ggtgaagatt
     5341 atgagtcaat catccgggtg cccttcaaaa tgatagaaat aacagaatga gtttcaaata
     5401 gttgttttct gacctctgtc gtacaggagc gggctgtgtc atatgtgatg atacagcccg
     5461 ttttgcttat ggtcctgaac tgctgttttt cctttccatg gctttgtttc ccgagtccag
     5521 tatttttttc aggatcctga agtacagaac ggaataaatc atcagggtaa tgcaccagag
     5581 gacaatgacg ttgacccagt aggtatccgt ttccctgccg aacaatttct ttaccggagc
     5641 ataaaaatgt gcctttatga aattgtgttc cggatccatg tacacaggat caagtttctg
     5701 gattatttca tccctgtata caattatttt ttcagtttca ttcttgttta tgacaaattc
     5761 ttccagggct tcattggcat agttatccct cattgccaga aaggctttac tgtctgcggc
     5821 ctgcagcctg gtgacaatgg catccttttt atcctttgca aggtttgaac aggctacata
     5881 gtatcttctg acttcttcgg tatagtccag tgcctccctg acaatttccg gggtgactcc
     5941 ctcaggtgtg agatgctcaa gatgcctgaa cttcagttgt ggcgtcagta ctgcctgttt
     6001 tgtgaattca ttgtaaacaa gtttcagttt cctgaaggat tcttcattta atgtacccct
     6061 ttccagatct gactgaattc tctccagcct gccctttatt tccgggtgca ggtagtcctt
     6121 cctgtatttg gcctggctca tcgccttgtc atagacataa aactgccttc catatagatt
     6181 gtccttgaac tgtttcacag ccagggcctc ataaccccat cgtgcagtta tcatttctcc
     6241 gtaaaaggga atggataccg gggaagacat ctgcggatta agtttctcga atttcaccat
     6301 tacaccgctt agcatgatct ggggaattac aaggaagggt atgagtatat aaattgtcac
     6361 aactgccttg aaactgtctg aaatgaccag tcccagcatg ttggcgcctg cccagcagct
     6421 gaaaagaaca agccagtact cgaagagcat tccccttatg ccggtgatcc agttccctat
     6481 aagcacgaag gaaaaggcct gtattgccga aatactgaac tggactatca ctttcgacat
     6541 aagatagctg ttccagctga gattgagaaa tgcttccctc ttaagtatct tgcggtcctt
     6601 gattatttcc tcggcactta ctgtaagacc catgaaaatg gccacaatca ccgacatgaa
     6661 gatataaacc gggagatttg cattttcata cagaatatac cggggaactg ctgagctttc
     6721 atcaaaatac ctgatcagat atgcaaggaa gagtgccaga accggagcct caagcagggt
     6781 gataagcaaa tactggatat cagatacctt tgagaggata tcgcgtttta cgaatacaat
     6841 catctgtctg accttattcg gtatgttgaa gttgatatcc ggaatggagt ttccgcgttc
     6901 cagctcttct tcctttttgt cagccagata gaattcatac cagtctgcag gggatattct
     6961 tctcgtatca gttgcctgac cgctctcgga atataccctg gtctcaagaa tattgaatat
     7021 ctgatcggga tttacatttc cgcagacata gcattcgctt tcgttgtagt tggtctgttc
     7081 aatctttttc ttgaaataat ctatcgattc cacaggattt ccgttgtaga cgggatatcc
     7141 tccggtatca agaataatca gcttgtcaaa cattttgaaa atatccgacg aaggctggtg
     7201 aatgacgatg aaaagcaatt tgcccttgcg ggtcagatct ttcagcaggt caagtatgtt
     7261 ttccgaatca cgtgatgaca gtcctgaagt gggttcatcc agaaagagta tcgatggctc
     7321 gcggatcagt tcaagtgaaa tattcagcct tttcctctgt ccgccgctga tctttttgtt
     7381 caggggactt cccactttca tgttccgtat atcatagaga ccgagattct tcagcacgtt
     7441 gtcaactttt tcaattattt caacgttgct tagatttccg aagcagagcc ttgcgttata
     7501 atacagattc tgaaacacgg taaggtcctc gatcagcagg tcatcctggg atacaaaacc
     7561 tatcagtccc ttgatctttt ccctttccct gtgaatattt atgccattta tcagaacttc
     7621 accatcggag ggctgatctg atccgttcag cacatgaaga agggtggact tacctgcacc
     7681 gctcgccccc attataccaa ccatccttcc agattcggtc ttaaaactca tatgatgaag
     7741 gccgatctca ccgctcctga acctgtactg caggttattc acttcataga cgactctgga
     7801 tgactggaaa tcctccatga gaaactgcat tgtgacatcc cagtaataga ccggtttcac
     7861 cttggagaag cgcagggatg atccttcact gaaaggatat accttgtcct cctgaagaag
     7921 ctgcacactc attgacaatt cacccgagcc cctgtagcgg aggaaataca tgtttaccga
     7981 gggtatgaaa aggatccaga tctgaccctt cagcagttcc ctgtggatat gcctggtctc
     8041 ccggaggctg cttgagggat tgccgtctat aagaaggagg ttctgtgaat caggaatctt
     8101 tacaatcgga gtcagaataa acaattcaat cagatcgaat tcttcctttt ttatattcag
     8161 cccctccgac agggttctga tgaaaccaag ctccatcgca ctcactgtcc tgtttcctga
     8221 cctgcagaat tcaagcgcct gaatgacaac gaatattctt tgttcatgat ctagtcttcc
     8281 ctgttcattt atctcgacac acaacctcca tatccttacc gcaatggcgc cttcgcgccg
     8341 tttggagctc actctttcaa gccgtttcct ggcttcttca taagcttcat catacctgga
     8401 aagatatttt tttacaagat cctgacttat aagcctgtac aggaatgatt caaccacctc
     8461 ccgtctttcg gtatcatcag cctgaggttt ggcaattatg gcaaataact gcatcaggat
     8521 ttcaagtatc ttctcactca ttcagacaag gatggttaaa tatacttatg ttatggatta
     8581 catgactttt acctgttaaa cgaagaaatg aaagcagggt ttcatcgggg cctgaactat
     8641 tacgctgcgg aatattcccg ttttccggaa atccagcgtc attctgacac aataaaccgg
     8701 tggtttaatg tcaaaaagtc ccttcgggct ttctggcaat ttttttgatg gaggaagcag
     8761 aaaaggagta tacatgaatt taaataattt cacattaaag gcgcaggaag cggttcagaa
     8821 agccttcaat ataaccaggg gaaaaggaca gcaggcagtt gaatgtgccc atcttctcaa
     8881 gggtgtcata agcgaggccg agagtatttc agattatctt ttcggcaagg ctggtattga
     8941 tacccggctt ttaatacggg atattgacag gctgattgat tcctatccga aagtatcggg
     9001 agccgaagca tatatttcac ctaatctgtc ggaggcattc cgcaaggcgg gtgatcatgc
     9061 ctcagacatg aaggacaagt tcataacgcc ggaacatctt attctgggag tgatgggaac
     9121 cggtgacgag agcgcgggaa tactcaggga tcacggagct accatgaagg gacttgtttc
     9181 tgcaatctca gaactgcgta aaggagcagt tgtcaacagc cagacatcgg atgacacctt
     9241 caactcactt gaacgatatg cattgaatct caatgaacgg gcacgttccg gcaagctgga
     9301 tccggttgtc gggcgcgatg acgagataag acggatatta cagatccttg aacggcgtac
     9361 caagaacaat cctcttatga taggggaacc
//
LOCUS       DAOSPX010000095         9310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7116_length_9310_cov_4.176121, whole genome shotgun sequence.
ACCESSION   DAOSPX010000095 DAOSPX010000000
VERSION     DAOSPX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7116_length_9310_cov_4.176121"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /gene="fucI"
                     /locus_tag="PLZ75_06265"
     CDS             complement(<1..847)
                     /gene="fucI"
                     /locus_tag="PLZ75_06265"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991938.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOS72039.1"
                     /translation="MGDFPKVGIRPVIDGRERGVRESLEKQTMDMAKSAAALISKNLK
                     YPDGKPVECVISDTTIGGVAEAAACAGKFAKHGVGVSLTVTPCWCYGSETIDMDRDIP
                     KAIWGFNGTERPGAVYLAAALAGHTMKGLPAFGIYGRDVQDAGDQTIPGDVSEKILRF
                     VRAGLAVSYIRGKSYLSMGSVSMGIAGSIVREDFFQEYLGMRNEYVDMSEFNRRLNEN
                     IYDRDEFERALAWTRKYCVEGTDLNEKPSSRARKDEEWEIVVKMTLIGRDLMVGNPRL
                     RELGFG"
     gene            1051..1911
                     /gene="lipA"
                     /locus_tag="PLZ75_06270"
     CDS             1051..1911
                     /gene="lipA"
                     /locus_tag="PLZ75_06270"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOS72040.1"
                     /translation="MEKRRRLPEWLKMQRASGENYSRVKHLAEDNNLHTICTSGNCPN
                     IGECWNSGTATFMILGDICTRACRFCNTRTGRPLPPDSDEGRRLAEAVAKLGLRHCVI
                     TSVDRDDLPDGGASFWADVILKVKEFNPGITIETLIPDFDGKPENLQKILDAGPDVIS
                     HNIETVRRLTPLIRTRAKYDRSLEVLRFISNSKKTAKSGFMLGLGENEAEIIETIDDL
                     LAAGCDVLTIGQYLKPSENHMEVVEYVPPGKFAEYRELALGKGFRAVESSPLVRSSFH
                     AGKHVRVENF"
     gene            1944..2660
                     /gene="lipB"
                     /locus_tag="PLZ75_06275"
     CDS             1944..2660
                     /gene="lipB"
                     /locus_tag="PLZ75_06275"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOS72041.1"
                     /translation="MKYNVKFEDIGLKDYKETWDYQAGIFEELKECKTGGKMTEGSGE
                     YRRPGTLILVEHPSVYTLGKSGSGNNLFLDSVQLRAKGAQFYRIDRGGDITYHGPGQI
                     VGYPIFDLEILKIGLKEYVFRLEEALIRTIGEFGLTGSRLQGGTGVWLDPGIKGRARK
                     ICAIGVKASRYITMHGFAFNVNTDLRWFDYINPCGFTDKGVTSLERETGLRQDMGEVK
                     TILKDRLRDQFGLEWINEEE"
     gene            2660..4390
                     /gene="recJ"
                     /locus_tag="PLZ75_06280"
     CDS             2660..4390
                     /gene="recJ"
                     /locus_tag="PLZ75_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOS72042.1"
                     /translation="MEKKWIIKERGDSAVVRQLAERLEISDSLANLMVQRGITTPGEA
                     QAFFYPSLDSLHDPFLMKDMNIAVERLSSAIKKNEKILVYGDYDVDGITAVALVYSFL
                     KEHYSNVDYYIPDRYKEGYGVSFQGLDYASENNCRLIITLDCGIKATEKIQYAKSRGI
                     DVIICDHHYPGDEIPMAHAVLDPKQPGCTYPYKELSGCGVGFKLIHAYSKIHAIPFTE
                     IIQYLDLVAVSIASDIVPITGENRVLAYYGLKQLNESPRTGLREVIREAEIIRTLTIE
                     DVVFRIGPRINAAGRMESGARAVELLISSDPKLAYGISKEIDNYNIERRNVDRLITTE
                     AMRMISEDKRTSNAKTTVLFNPSWKKGVIGIVASRLIETYYRPTVILTESNGFATGSA
                     RSVQGYDLYQAIESCSDLLESFGGHMFAAGLTLKKENIRPFAERFEQYVNRTITEDQL
                     QPRIFIDAGLSFSEIDDEFFRVMNQFQPYGPENMAPVFMSRNVFDTGSGRMVGSSGEH
                     LKLELYQEGDPLKILPAIAFNQAHRFEYIRRGEPVDICYSIELNEFRGVRSLQLNIKD
                     IKSARVAAPS"
     gene            4462..5751
                     /locus_tag="PLZ75_06285"
     CDS             4462..5751
                     /locus_tag="PLZ75_06285"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOS72043.1"
                     /translation="MKIKRLLPLTVLLIFSVSLCGQGLQQAKSGSLLRAGTARTDITP
                     ALPVKLYGYASRKTYSEGVHDPLSARVVVFENNGRRLLLVSTDIGSYGNEVYHTIRKA
                     ITDRFGFSESEIFLSTIHTHSSPTLTVNSQTGDPNNIEYTKFLQERLLAAIDEAIRDL
                     RPVTAGAGKGSSPVGANRREMQADGSIKLGRNPEGITDKEVVVMKIATPDGRPLAALY
                     DYATHSTSLGPRNMLVSGDVLGLSAQFVEKILGKDVIAPVFTGASGNIDPWYRVLPEF
                     SSEPGWIPEPVLLGNLLGVEVVHVFRGIRETDLTGDISTDFAILECPRKITAEAPSGT
                     DQPSVVPVHITAARIGKDVAFIGFNVEMLTEIGMEIKAGSPFRYTFIITHCNGSAGYL
                     APAGLYKEGGYEVESSRFAIGSSDMVVKKALRMLYDL"
     gene            complement(5822..7795)
                     /locus_tag="PLZ75_06290"
     CDS             complement(5822..7795)
                     /locus_tag="PLZ75_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72044.1"
                     /translation="MKPHLTILILAVLLCVSVLIHGQEIRQSDMTDIAEELAGDESDP
                     ESAAIFLERLQELAENPVMINSAEENELARLFFLTDFQIRALSDHIRSTGSILSLYEI
                     ASIPGFDRRTAEIMNPLISLRESGRKININSKFMSRTLSSFIIKPAGSDSNYPGSAWK
                     ILVRHRTEKGPFTAGFTCEKDPGEKFLSGSPPLPDFLSAHLAFSGNGLVRKVIAGDYS
                     AKFGQGTALNTGIHTGLPLTAAGYFPSRNEIKPYTSVDENNFFRGAACELASGMAGLS
                     LFFSHNRIDAATEYSGDSSELYIRSLYKTGLHNTPSAILKKDAAAETAYGTNLSLNFR
                     KVTAGMNFHRTILSLPFTDSLAKAEKLFDFTGRRKDILSAYYTASIGRSLLFGELSYG
                     GSNRSALVQGFTLRASGRLTINGLYRYYSPGFGGLHSRGPGTGSSAANEEGILGNFTF
                     EAARYLFISAGYDLCRHPWLKYRTSFPSLSKRYEVKLRYLPPDRLNVEMSYCNRSSVN
                     NEKKDRGIAGRKTASSGTLRTVFRYSPEKNLSLNFRIDCKSVRESGSRGYLMSQDLKY
                     SFRTLPLTFWTRFCLFSTDDWDSRLYVYEHDLLYSYSIPAFSREGSRMYLMTEWNFSS
                     FARIRLKYAHTSFHDNNPPEDLNELRMQLIMRF"
     gene            complement(7905..8729)
                     /locus_tag="PLZ75_06295"
     CDS             complement(7905..8729)
                     /locus_tag="PLZ75_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72045.1"
                     /translation="MYSLHKIPVSLIVFSLGLALSGNNPHSLPAGAADAGRGSVCVMK
                     PGFWSSFRNPALLASFHDFSAGINYENRFNISELGSRTAAMIIPAGKPSLGIIYSHFG
                     FSHFNRQTFGIACGTILWENISSGVQIDYFFEKVSEKYDNIHSVTFEGGLTIRPAENI
                     NIGVHVFNPLPNSLRKRTLPSFIRAGAGIMLNENLFAGAEAEMCTGSTLILRTGFEYG
                     LTDDLFVRGGFSSRNSSFSFGLGYSFDRISLDMSFAGHERLGITTSLSLIFLLRDQ"
     gene            complement(8765..9271)
                     /gene="purE"
                     /locus_tag="PLZ75_06300"
     CDS             complement(8765..9271)
                     /gene="purE"
                     /locus_tag="PLZ75_06300"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOS72046.1"
                     /translation="MKPLVSIIMGSLSDLDIMEKTAQILNDFRIPFEINALSAHRTPG
                     EVEEFARSAADRGIRVIIAAAGMAAHLPGVIAAMTPLPVIGVPINASLEGLDSIFSIL
                     QMPPGVPVATVGVNAAQNAGILAAQIIAAGNKDVMKEVIRYKEDLKKKIVSANEEIKN
                     IKFPFKTN"
BASE COUNT         2393 a         2157 c         2351 g         2409 t
ORIGIN      
        1 cgccgaatcc caattctctg agcctgggat taccgaccat aagatcacgt cctatcaggg
       61 tcattttgac cacgatttcc cattcttcat cctttcttgc ccttgaactc ggcttctcgt
      121 tcaggtctgt cccttcaacg cagtatttcc ttgtccatgc cagtgccctt tcaaattcat
      181 ccctgtcata gatgttttca ttgagcctcc tgttgaattc gctcatatcg acgtattcat
      241 tccgcattcc aaggtattcc tggaagaaat cctctcttac tattgaaccg gcaattccca
      301 tggagactga tcccattgag aggtatgact tgcctctgat gtacgaaaca gccagtccgg
      361 ccctgacgaa gcggagtatc ttttcactta cgtcgccggg gattgtctga tcgcctgcat
      421 cctgtacatc acggccatag atgccgaatg ccggaagtcc cttcatggta tgcccggcaa
      481 gtgcagcagc aagataaacg gcaccgggcc tttcagtgcc gttgaaaccc cagatggcct
      541 tgggaatgtc cctgtccata tctatggttt cacttccgta acaccagcag ggagttaccg
      601 tcagacttac accaaccccg tgctttgcga acttgccggc acaggctgcg gcctcggcaa
      661 cacctccgat cgtggtatcg gaaataacac attcaacagg ttttccgtca ggatatttca
      721 ggttttttga aataagggca gcggcggatt tcgccatgtc catggtctgc ttttcaaggg
      781 attccctgac acccctttcc cgtccgtcaa tgacaggacg aattcccact ttcggaaaat
      841 cgcctataac tgtctgaaag tcagcattta ctttaaattc attgtttgcc atgtatctgg
      901 tatttgtggg tttgattttt tcttatcaaa caaatatagt gttttatctt tttaagctga
      961 tgattaataa ataccgggat gaagaattta ttttttcccg cccttattga tgaggaattt
     1021 tgtactttta tcgtccttaa aagagcaatg atggaaaagc ggagacgatt gccggaatgg
     1081 cttaaaatgc agagggcttc gggtgaaaat tacagcaggg taaagcatct ggctgaggac
     1141 aataatcttc ataccatctg tacaagcggt aattgtccga atatcgggga gtgctggaat
     1201 tcgggcactg ctaccttcat gattctcggg gatatctgta cccgggcgtg caggttctgc
     1261 aatacccgga cgggcaggcc ccttccgccc gacagtgatg agggcaggag gctggctgaa
     1321 gcggtggcaa agctgggcct caggcactgt gtcataactt ccgttgaccg tgatgacctg
     1381 cctgacgggg gcgcttcatt ctgggcggat gtgatactga aagtgaaaga atttaaccct
     1441 ggcataacaa tcgaaacgct tattccggat tttgacggca agccggaaaa ccttcagaag
     1501 atcctggatg ccggcccgga tgttatttct cacaatattg aaacggtcag gcgcctgacg
     1561 ccgcttatca ggaccagggc aaagtatgac agaagtctcg aagtgctcag gttcatttca
     1621 aacagcaaga aaacagccaa atcggggttt atgctcggcc tcggggaaaa tgaagcggag
     1681 ataattgaaa ccattgatga tcttctggcc gccggatgtg atgtcctgac catcgggcag
     1741 tatctgaaac cttcggaaaa ccatatggaa gtcgttgaat acgttccgcc gggcaaattt
     1801 gcagaatacc gggaactggc cctggggaaa ggcttccggg ccgtggaaag cagtcccctc
     1861 gtgcgatcgt cgtttcatgc cgggaaacat gtaagagtgg agaattttta gtatcttggg
     1921 ctcacccaaa tagtttagat gtattgaaat ataatgttaa attcgaggat attggcctga
     1981 aagattacaa ggaaacatgg gattaccagg cagggatctt tgaagaactt aaagaatgca
     2041 agacaggggg gaagatgacg gaagggagcg gggagtaccg caggcccggc actcttatcc
     2101 ttgttgagca tcccagtgtt tataccctcg gaaagagcgg atcgggaaac aatcttttcc
     2161 tcgacagtgt tcagctgagg gcaaagggag cacagttcta caggatcgac agggggggag
     2221 atatcacgta tcacggtccg ggacagatag taggatatcc cattttcgac ctcgagatat
     2281 tgaaaatcgg gttgaaggaa tatgttttca ggctggagga agctttaatc cgtacaatcg
     2341 gggaatttgg actgacggga tcacggcttc agggaggaac cggtgtctgg cttgatcccg
     2401 gaataaaagg aagggcacgg aaaatatgtg ccataggagt gaaagccagc agatacatca
     2461 ccatgcatgg ttttgcattc aatgtaaata cagatttgcg gtggtttgat tatatcaatc
     2521 cctgtggctt tactgacaag ggggttactt cccttgaaag ggagacggga ctgaggcagg
     2581 atatgggaga ggtgaagact atccttaagg acaggctgag ggatcagttc ggcctggaat
     2641 ggatcaatga ggaggaatga tggaaaagaa atggataata aaagagaggg gcgacagcgc
     2701 tgttgtgagg caacttgcgg aaaggctgga gatttctgat tccctggcca atctgatggt
     2761 tcaaagaggc attacaacac ccggggaagc ccaggctttt ttttatccta gtctggacag
     2821 ccttcatgac ccgttcctta tgaaggatat gaatattgcc gttgaaaggc tttcctcggc
     2881 aataaagaaa aatgaaaaga tcctggttta cggcgattat gatgttgacg ggattacggc
     2941 agttgcactg gtttattcct ttctgaagga acattattcc aatgtggatt attacattcc
     3001 cgacaggtac aaggaaggat acggagtgtc gttccagggc ctggattatg cttcggaaaa
     3061 taactgccgc ctgatcatta cccttgattg cgggatcaag gcaacggaaa agatacagta
     3121 tgcaaaaagc agggggattg atgtgataat atgtgaccat cattacccgg gtgatgaaat
     3181 accaatggca catgccgttc tggatcccaa gcagccgggc tgcacctatc cgtacaagga
     3241 actgtcggga tgcggtgtcg gattcaagct gatacatgca tattcaaaga tacatgccat
     3301 tcccttcacg gaaataatac agtatctcga cctggtggct gtaagcattg cctcggacat
     3361 tgtgccgata accggggaga acagggtact ggcatattac gggctgaaac agctcaatga
     3421 atctccgcgc acgggcctgc gggaagttat ccgtgaggct gaaatcatcc gtacactgac
     3481 aattgaggat gttgttttcc ggattggtcc gagaatcaat gccgccggga gaatggaaag
     3541 cggggcaagg gccgttgaac tgctgatctc aagtgatccg aagctcgctt acgggataag
     3601 caaggaaata gacaattaca atattgaaag gagaaacgtt gaccggctga taacaaccga
     3661 ggcaatgagg atgatttccg aagacaagcg tacatcgaat gcaaaaacaa cggtgctttt
     3721 caacccctcc tggaaaaagg gagtcatagg aatcgtggct tcacgcctta ttgaaacata
     3781 ttaccggcca acagtcattc tcactgaatc aaacggattt gcaaccggtt cagcacgatc
     3841 ggttcagggt tatgacctct atcaggcaat tgaatcctgc agcgatctcc tggaaagttt
     3901 cggcggacat atgtttgctg caggactgac actgaaaaag gaaaacataa ggccgttcgc
     3961 ggaaaggttt gaacagtatg taaaccgtac aataacggaa gatcagctgc agccgaggat
     4021 attcattgat gccggactct cattcagtga aatagatgat gagttttttc gtgtcatgaa
     4081 ccagttccag ccctatggac ccgagaatat ggctcccgtt ttcatgtcgc ggaatgtgtt
     4141 cgacacagga tcgggacgca tggtcggatc cagcggggaa catctcaagc tcgagctgta
     4201 tcaggaaggg gaccctctga agattctccc ggcaatcgcc ttcaatcagg cccaccgctt
     4261 tgagtatatc cgcaggggag agcccgtaga catatgctat tcaatagaat tgaatgaatt
     4321 caggggagtc aggagccttc agctcaatat aaaggatatt aagtctgccc gggttgccgc
     4381 cccctcttga ctccttccgg gtataaacag gcagggaaac tgaaatattt ttccgaaatt
     4441 agttatataa aattttccgc catgaagatc aaaagactgc tgcccctcac cgttttgctt
     4501 attttttctg tatctctctg cggacaaggc ctgcaacagg caaaatccgg atcccttctc
     4561 agggccggaa cagccaggac ggatatcact ccggctcttc ccgtcaagct ttacggatat
     4621 gcctccagga aaacctattc tgaaggtgtg catgatccgc tgagtgcccg tgttgttgtt
     4681 tttgaaaaca acggcaggag gctgctgctt gtttctactg atatcggatc atacggaaac
     4741 gaagtatatc atacaatacg aaaagcaatt actgacaggt tcggattcag tgaatcggaa
     4801 atattcctgt caacgatcca tacccacagt tctcccacac tcacagtcaa ttcccagacg
     4861 ggtgacccga acaatattga atacacgaaa ttcctccagg aaaggctgct ggctgccata
     4921 gatgaagcca tccgggattt aaggcctgta acggccggag cagggaaggg ttcgtcacct
     4981 gtgggtgcca accgccgcga aatgcaggct gacggttcaa taaagcttgg aaggaatcct
     5041 gagggcatta ccgacaagga agtggtggtg atgaaaattg ccactcccga cggcaggccg
     5101 ctggctgctc tttatgacta tgcgacgcat tccacctccc tgggaccgcg gaacatgctg
     5161 gtcagcggtg acgtcctggg cctttccgcc cagtttgtag agaagatact ggggaaagat
     5221 gtgattgctc ctgtttttac aggtgcttca ggtaacatag atccgtggta cagggttttg
     5281 ccggaattca gcagtgaacc cggctggatt cccgaaccgg tgcttctggg caatctgctc
     5341 ggggtggagg ttgttcatgt attccgggga atccgtgaaa cggaccttac aggagatatc
     5401 tccactgatt ttgccattct tgaatgcccc cggaaaatta cggctgaagc tccatcagga
     5461 acagaccagc cttcagttgt ccccgtgcat ataacggcag cacgcatagg gaaggatgtt
     5521 gcctttatag gattcaatgt tgaaatgctc accgaaatag gaatggaaat aaaagccgga
     5581 tcacccttcc ggtatacctt cataataaca cactgcaacg gatcggccgg ataccttgct
     5641 cccgccggcc tgtacaagga aggaggatat gaggttgaat cctcaagatt cgcaatagga
     5701 tcgtcagaca tggttgtgaa aaaagcactg agaatgcttt acgacctgta ggagactgct
     5761 gtcatgagca atggaaatgc cggttccggc ccggtcttta ttattgcctg cggcggcctt
     5821 tttaaaatct cattattaac tgcatcctga gctcgttcag gtcttccggc ggattattgt
     5881 catgaaatga tgtatgggca tatttcagcc tgatacgggc aaaagaggaa aaattccatt
     5941 cagtcatcag atacatcctt gaaccttccc ttgaaaatgc cgggatgctg taactgtaaa
     6001 gcaggtcatg ttcataaaca tatagccggg aatcccagtc atcggtactg aacaggcaga
     6061 accttgtcca gaaagtaagg ggaagggttc tgaaagaata tttcagatcc tgtgacatca
     6121 ggtatcccct gcttccgctt tcacggaccg atttgcagtc gatcctgaaa ttcagggaca
     6181 ggttcttctc cggggaatac ctgaatactg tcctgagtgt tccggaggaa gctgttttcc
     6241 tcccggcaat tcccctgtcc tttttttcat tattaacgga cgatctgttg cagtacgaca
     6301 tttcaacatt cagtctgtcc ggaggcagat atctcagttt tacttcatat ctttttgaca
     6361 acgacgggaa acttgttctg tatttcagcc acggatgacg gcaaaggtcg tatcctgcac
     6421 tgatgaagag atatcttgct gcctcgaaag tgaaattccc cagtatccct tcctcgtttg
     6481 cagccgagga gccggttccg ggccctcttg aatgcaggcc cccgaagccg ggggagtaat
     6541 aacggtaaag tccgttaatg gtcagccttc ctgaggctct cagggtaaaa ccctgaacga
     6601 gtgctgacct gttgcttccc ccgtatgata attcgccgaa aagcaatgac ctgccaatgg
     6661 atgccgtgta gtaagctgaa agtatatctt ttcgcctgcc ggtgaagtca aacaacttct
     6721 cagctttggc cagggagtct gtaaacggaa gtgaaaggat ggtccggtga aagttcatgc
     6781 ccgctgtcac tttcctgaaa ttcaggctca ggttagtccc gtatgccgtt tcggcagcgg
     6841 catccttttt caggatagcg gacggggtgt tgtgcagtcc tgttttataa aggcttctga
     6901 tatacagttc tgatgaatca cctgaatatt ccgttgcggc gtcaatcctg ttatgtgaga
     6961 aaaacagcga cagcccggcc attccgcttg ccagttcaca tgcggcaccc ctgaaaaaat
     7021 tgttttcatc cacagaggta tagggtttta tttcattccg tgaaggaaaa tatccggccg
     7081 cggtaagcgg caatccggta tggatgccgg tattgagtgc cgtaccctgg ccgaattttg
     7141 ccgaataatc gcctgctatc acttttctta caaggccgtt gcctgaaaag gccagatggg
     7201 cggaaaggaa atcgggaagg ggaggagagc cggaaaggaa tttctccccg ggatccttct
     7261 cacaggtaaa tcctgcagtg aaaggaccct tttctgttct gtgcctgaca agtattttcc
     7321 atgctgatcc gggatagttg ctgtcagatc ccgcaggctt gatgatgaaa ctggaaaggg
     7381 ttctgctcat gaacttagaa tttatattaa ttttccgtcc tgattctctg agacttatca
     7441 gcggattcat gatttctgcc gttctgcggt cgaatcccgg tattgatgcg atctcgtaaa
     7501 gggacaggat actgcctgtt gatctgatgt gatcgctgag tgccctgatc tgaaagtctg
     7561 taagaaagaa caggcgtgcc agttcatttt cttcggccga attgatcatg acgggattct
     7621 ctgccagctc ctggagacgt tcaagaaaga tggctgctga ttcaggatcg ctttcatccc
     7681 ctgccagctc ctcggcaatg tctgtcatgt cgctctgcct gatttcctgg ccgtggataa
     7741 gcactgaaac acataacagg accgccagaa tcaatattgt cagatgaggt ttcatagaga
     7801 tttcccttca ttcagaattt cctgtccctg tttccattta cggctcttca tcacggagtc
     7861 ccttgccggt aatttcttaa atgcttttcc ctgtatgatt tttctcattg atcccttagc
     7921 agaaatatca gcgaaagaga agtggttatt cccagccgtt catggccggc aaagctcatg
     7981 tcaagactga tgcggtcgaa actgtatccc agtccgaagg agaatgacga attccggctg
     8041 ctgaagcctc ctcttacaaa aagatcatcc gtcagcccgt attcaaaccc ggttctcagt
     8101 atcagtgtgc ttcccgtgca catctccgct tcagcacctg caaacaggtt ttcattgagc
     8161 attatccccg cacctgccct gatgaacgag ggcagggttc ttttgcgcag tgagtttgga
     8221 agagggttga aaacatggac cccgatgttt atgttttccg cgggtcttat cgtcaaccct
     8281 ccctcgaagg tgacagaatg gatattatcg tatttttccg aaaccttctc aaagaaatag
     8341 tctatctgca cgccggagga aatattttcc cacagtattg ttccgcatgc aataccgaaa
     8401 gtttgtctgt tgaaatgtga aaaaccgaag tgggagtaga ttattcccag cgagggtttt
     8461 cccgcgggga ttatcattgc ggcggtacgt gagcccagtt cgctgatgtt gaagcggttc
     8521 tcataattta tccctgccga gaagtcatgg aaggatgcca gcagtgcagg atttctgaaa
     8581 gaggaccaga agccaggttt catgacacat accgatcccc tgcccgcatc tgctgctcct
     8641 gccggaagac tgtgaggatt attgccggaa agggcaagac cgagggaaaa cactatcagg
     8701 gaaacaggta ttttgtgcag cgagtacaaa ctgtttcaat atctgacagc aatataggaa
     8761 aaaatcagtt tgttttaaag gggaatttta tattttttat ttcctcattg gcactgacaa
     8821 tcttcttttt cagatcttcc ttgtatctga tcacttcctt catcacatcc ttatttcctg
     8881 cagcgatgat ctgggcggcc aggatacctg cattctgtgc ggcgttgacg ccaacggttg
     8941 ccaccggtac gccgggaggc atctggagta ttgaaaatat tgaatcgagg ccctcaagcg
     9001 aggcatttat gggtacgcct atcacaggaa gaggagtcat ggctgcaatg acaccgggca
     9061 ggtgagctgc cattcctgct gcggcaatga taaccctgat acctctgtct gctgctgatc
     9121 tggcaaattc ttccacttcc ccgggtgtcc ggtgagctga aagtgcgttt atctcaaaag
     9181 ggattctgaa atcattcagt atctgcgccg ttttttccat aatgtccaga tcggacaggc
     9241 tgcccattat tatgcttacc agtggtttca tgatattgtt acaattaact tcctgacaag
     9301 gacaaaaata
//
LOCUS       DAOSPX010000096         9290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7128_length_9290_cov_3.976611, whole genome shotgun sequence.
ACCESSION   DAOSPX010000096 DAOSPX010000000
VERSION     DAOSPX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7128_length_9290_cov_3.976611"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLZ75_06305"
     CDS             complement(<1..399)
                     /locus_tag="PLZ75_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HOS72047.1"
                     /translation="MKILYYDCFSGISGDMNLGAMIDLGVDKDFLIGELKKLNLPGWE
                     LLVERDQRHGISGTKVTVRLTGHDHGHSHVHDHDHGHGHSHGHDHEHNHYHDHNHDHS
                     HAHDHEHGHDHGHSHSHSHSHDHADRNLTDI"
     gene            571..1494
                     /locus_tag="PLZ75_06310"
     CDS             571..1494
                     /locus_tag="PLZ75_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS72048.1"
                     /translation="MKRRDFIKTSAGAGLGAGFVLGSGGHDGFFRAVQARSDKYDMIA
                     VMGGSPEAMFDLGIQELGGMGAFVKKGQKVLVKPNIGWDVLPELAANTNPLLVRRVIE
                     HCLKAGAREVYVFDHTIDNWVNCYKNSGIERAAKLAGAKVVPGASEKYYQEVNIPGGI
                     KLRKTKVHELVLDTDVFINVPVLKDHNSVRMTCSLKNMMGVVWDRAYWHKNDLNQCIV
                     DYAMFHRKPALNVIDCYNVMVQHGPQGVSKADVVQMKSLILATDWVAGDTAASRMLGT
                     QTSRIEYIPMAHKLGLGTMDIDTLNIKRIKM"
     gene            1501..3060
                     /locus_tag="PLZ75_06315"
     CDS             1501..3060
                     /locus_tag="PLZ75_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS72049.1"
                     /translation="MRSAFLRSFRIFFSAFIVICFIVVFSDFKYIIPSKYINLLLWFQ
                     FVPSAVKFYDLFTLATAGFAGVLVLTLITGRSYCSFLCPLGIAQDINSRIGGRIRRKF
                     RRYGYKKPFTVLRYLILVVVLIVTFFSGIYLLTLLDPYAIFGRSVTMFIKPAVIVMNN
                     FLVWVLGKFDNYSLSHVAIGGYKPVSYAIPAVFFLLVVSLSLTKGRLYCNMICPVGTL
                     LGFLSKLSLLRIRFDVDKCTKCGRCSLGCKSSCIDFIRYRVDVSRCVVCFNCINTCPE
                     KALSWGPVRFRKKTHTTDEGKRKFIAGSLAALFGVPAILRSQDSKTPIPQILSTVPEN
                     RTCPVAPPGAGSLEEFNKTCIACSLCITVCPNEVLVPAFMQYGIAGIMQPVMDYKKSF
                     CIYNCTRCTEVCPTYSLRPLVIEAKKLTQLGKVNFIKDNCIVKTEKTACGACSEACPT
                     KAVYMIPYEGNLLIPETNTDICIGCGHCEFACPTKPYKAIFVDGNPVHQTAEKPVEEE
                     PGIKAPAEFPF"
     gene            3087..3500
                     /gene="mce"
                     /locus_tag="PLZ75_06320"
     CDS             3087..3500
                     /gene="mce"
                     /locus_tag="PLZ75_06320"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOS72050.1"
                     /translation="MNPAHIEHIGIAVRNLESAIGFYERILGLKCHKIEEVADQKVRV
                     AFFRIGQTSVELLEPTDNDSPVARFIEKRGEGMHHLAFAVEDIEKKLDEAGRKGIRLI
                     DKVPRRGADGKDIAFIHPESTGGVLIELCEKRNGK"
     gene            3524..3685
                     /locus_tag="PLZ75_06325"
     CDS             3524..3685
                     /locus_tag="PLZ75_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOS72051.1"
                     /translation="MEKYECEICGYIYDPEKGDPEGDIKPGVPFSDLPDDYLCPVCAA
                     GKEEFSLLE"
     gene            complement(3700..5175)
                     /locus_tag="PLZ75_06330"
     CDS             complement(3700..5175)
                     /locus_tag="PLZ75_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOS72052.1"
                     /translation="MSVFKGLKPEALWQYFEEICRIPRLSGNEGKIRQYLLEFAEHHN
                     LEAKEDHAGNVLITRPSHPDHLNRKIVVLQSHLDMVGEKTAGHPHDWTKDPVIPLSDG
                     EWIRAGGTTLGADDGIGIAAQMAVLTDKTLRAGTIECLFTVDEESGMSGAKELTEDFF
                     RGRILLNLDSEDEGILFIGSAGGMDTVGTMRYTREAVPPGYVGLEISLSGLQGGHSGD
                     EIHKGLGNSVKIMTAILTELDREHGIRLSKFDGGNLRNAIPREAYAVILCRKSGSENI
                     ISHLKTIETGLRKKFGDLERNLKLEAAECTMPSHVMDRGSQDGLLRALSCCPHGVIAW
                     SEDMKDLVETSSNLASVKFEPAGFVTIVSSQRSSFEASKAEASAMVEDCLKQAGATVT
                     KSAGYPGWAPDINSEILGITRQAYRRLFGKDPEVKAIHAGLECGLIYEKFRKMDMISF
                     GPTIRGAHTPDEKIEIRTVPMFWNLLMEVINNIPLKSGYSI"
     gene            complement(5195..7108)
                     /locus_tag="PLZ75_06335"
     CDS             complement(5195..7108)
                     /locus_tag="PLZ75_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS72053.1"
                     /translation="MKSIVQFFEESVEKFNSNVYLWEKTQDKYEGTTYGETRRQVYEF
                     AAGLIALGVKKGDRISLLSEGRNTWVIGELGILYTGALNVPLSTKLNPDEIKFRLNHS
                     ESSMLLVSTLQYPRVKQVISECRTLEKIIHLDTQEEYEDDEIHFNEVRKLGREWLESP
                     ENVAKFEEISKGIKPSDFANICYTSGTTADPKGIILTHGNYITNVYQSYSLMDIPPFY
                     KTLLILPWDHSFGHTCGIFAFMGRGASIASVKTGRTQLETLRNLPVCLREIKPNLLLS
                     VPAIAKNFRKNIEKGIKEKGRLTEFLFNHALKISYSYNKEGWNRGQGLQKLKKPLLKL
                     YDKVIFSKIREAYGGELDYFIGGGALLEIELQRFFYALGIPMYQGYGLSEASPVISSN
                     SAARHKLGSSGVLVNNMGFKICDDDGNELPAGEKGEIVIKGGNVMHGYWKNDAATKEA
                     IRDGWLYTGDMGYITEDGYLYVLGRFKSLLIADDGEKFSPEGIEEAISEQSKYIDQCM
                     LYNNQKPYTVALIVPNQHALKIYLEEKDLTADTEEGKRVILNLLESEVNEYRTNGKYG
                     KMFPQRWLPVAIGVLEEGFSEDNGLMNSTMKIVRGKIMEKYNELINFLYTPDAKVITN
                     ERNLEEVEKMKLG"
     gene            7295..7762
                     /locus_tag="PLZ75_06340"
     CDS             7295..7762
                     /locus_tag="PLZ75_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72054.1"
                     /translation="MRHPTAILLILAIAMISSCKFLKEKTFLGKKDRLLAEWQARQDS
                     IRVADSIRHVHDSLLAIENARLDSLRLAEEEKHALESRYNIIVGSFITPAYAEALADD
                     YRRRGYDVRIIHRENSRFSLVSAESHESFRRAVSRIQQFQDTVAIDSWIYTLK"
     gene            complement(7771..9036)
                     /locus_tag="PLZ75_06345"
     CDS             complement(7771..9036)
                     /locus_tag="PLZ75_06345"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOS72055.1"
                     /translation="MKIDILLGLQWGDEGKGKIVDAISPQYDVVARFQGGPNAGHSLE
                     FNGKKHILHLIPSGIFHPDKINIIGNGVVIDPAVFKKETENLGIPPDELVKRLVISSR
                     ANLILPGHRILDAAYENTRGDSKIGSTLKGIGPAYTDKIARNGLRAGEIFSTDFRELY
                     NQRFRNHMSILKNHDFSFDPEEYEADWFEGVEIMKKFRIVNTEYFINDLVLKGRKILA
                     EGAQGTMLDIDFGTYPFVTSSSTVSAGACTGLGISPGHVGEVFGIFKAYCTRVGSGPF
                     PTELTDDTGNTLRNKGNEFGSTTGRPRRCGWLDIPALKYAIMINGVTRLFMTKSDVMS
                     GFDNVRVCTSYMWENRECEEIPFDSRKIQPVYTVLPGWKEEIREMRNFGDLPVNLKGY
                     IEFIEYHTGVPVTMVSVGPDRRETIFRNQ"
BASE COUNT         2321 a         2134 c         2112 g         2723 t
ORIGIN      
        1 tatatctgta agattcctgt cggcatggtc gtggctgtgg ctgtggctgt ggctgtggcc
       61 gtgatcatgt ccgtgttcat gatcatgtgc atgactgtgg tcatgattat ggtcgtggta
      121 gtggttgtgc tcatgatcgt gcccgtggct gtgcccgtgt ccgtgatcat ggtcgtgtac
      181 gtggctgtgc ccgtggtcat gtccggtaag cctgactgtt acttttgttc cggatatacc
      241 gtgtctctga tccctttcaa ccagcagttc ccacccgggg agattcagtt tcttcagttc
      301 cccgataaga aagtccttgt caactcccag gtcaatcatt gctccgaggt tcatatctcc
      361 gcttattccc gagaaacaat cataatacag tattttcatt tactaaaagt atttttatat
      421 ggtacaacaa ttaaaattgc ataaggttaa aagcttttca ttcattccgg ggactaatat
      481 acgataacaa actgttaaag attagtaaaa aaaatcgtat tttgtggcac ttattgtcgg
      541 gtcaggatta atttttatta ttttgatgca atgaaaagaa gagattttat aaaaacgtct
      601 gccggagcgg gcctgggagc aggatttgta ttgggttcag gcgggcacga tggttttttc
      661 agggcagttc aggccagatc cgacaaatat gacatgattg ctgttatggg aggcagtccg
      721 gaagcaatgt tcgatctcgg gatccaggaa ctgggaggga tgggggcttt tgtgaagaag
      781 ggccagaaag tacttgtgaa gcccaatata gggtgggatg tgcttccgga gcttgcggcg
      841 aacaccaatc ctcttctggt cagaagggtt atagaacatt gcctgaaggc aggagccagg
      901 gaagtatatg tatttgacca caccattgat aactgggtta actgttacaa aaactccggc
      961 attgaaaggg ctgcaaagct ggccggtgcc aaggtggtcc ctggagcgag cgagaagtat
     1021 tatcaggagg ttaacatacc cggcgggata aaactcagga aaaccaaggt acatgaactg
     1081 gttcttgata cggatgtgtt tattaacgtt ccggttctca aggatcacaa ttcagtgagg
     1141 atgacatgca gcctcaagaa catgatgggt gttgtatggg acagggctta ctggcataaa
     1201 aatgatctga accagtgtat tgtggattac gcaatgtttc acaggaagcc tgccctgaat
     1261 gtgatcgact gctacaatgt aatggttcag catggtcctc agggcgtttc aaaggccgat
     1321 gtcgttcaga tgaaatcact gatactggca acagactggg ttgccggtga tacagctgca
     1381 tcaaggatgc ttggaacaca aacttccaga attgaatata tacccatggc acataagctg
     1441 gggctgggca caatggatat tgacacattg aatatcaaaa gaataaaaat gtaataatcg
     1501 atgaggtcag catttttaag aagcttccgg atattttttt cagctttcat tgtcatttgc
     1561 ttcattgttg tcttcagtga tttcaaatac attattccat caaaatacat caatcttctc
     1621 ctgtggtttc agttcgttcc gtcagcagtg aagttctatg atctcttcac ccttgccacc
     1681 gctgggtttg ccggggttct cgtactgaca ctgattaccg gacgcagcta ttgttccttc
     1741 ctgtgccccc tgggaatagc ccaggatatt aacagcagga taggaggccg gatcaggagg
     1801 aaattccgca ggtacggata caagaaaccg tttacggtac tgcgttacct gatccttgtt
     1861 gttgtactga ttgtgacgtt tttttcaggt atttaccttc ttaccctgct tgatccctat
     1921 gccattttcg gaaggtccgt taccatgttc attaaacctg cagtgattgt aatgaataat
     1981 ttccttgtat gggtactggg aaaatttgac aattacagtc tcagccatgt ggcaatcggc
     2041 ggatacaagc ctgtttccta tgcaataccg gctgtctttt tccttcttgt tgtgagtctg
     2101 tctcttacaa aaggccgcct gtactgtaac atgatctgtc ccgtgggtac actgctgggg
     2161 tttttatcaa aattatcact tctcaggatc aggtttgatg tggacaaatg taccaaatgc
     2221 ggcaggtgct cacttggctg taaatcctcc tgcatagatt tcataagata cagagttgat
     2281 gtttcaagat gtgtggtttg cttcaattgt atcaatacat gtcctgaaaa ggctctttcc
     2341 tgggggccgg tccggttcag gaagaaaacc cacaccaccg atgagggcaa gcggaaattc
     2401 attgcaggtt cacttgccgc cctgttcggt gttccggcca ttttgaggag tcaggacagc
     2461 aaaacaccca taccgcaaat cctttcaact gttcccgaga acaggacatg cccggttgct
     2521 cctccgggcg cgggatccct ggaagaattc aataaaacct gcattgcgtg ctcgctttgc
     2581 ataactgtct gtcccaatga agtgctggtt cccgctttca tgcaatatgg cattgccggg
     2641 attatgcagc cggtgatgga ttataaaaaa agcttctgta tatacaactg caccagatgc
     2701 acggaagtct gtcccactta ttcccttcgt cccctggtaa ttgaggccaa gaagcttaca
     2761 cagctcggga aggttaattt tataaaggac aattgcattg ttaaaacgga aaaaacggct
     2821 tgtggtgcct gttccgaggc atgtccgaca aaagctgtct atatgatacc ctatgaaggc
     2881 aatcttctca tccctgaaac caatacggat atctgcattg gttgcgggca ttgtgagttt
     2941 gcatgtccta caaaacccta caaggcgata ttcgtggatg gcaatccggt gcatcagacc
     3001 gcggaaaaac ccgttgagga agaacccggt ataaaggccc cggccgagtt ccccttttaa
     3061 tccgatgaat ctgctgataa tccccgatga acccggcaca tatagaacat ataggaattg
     3121 cagtaaggaa ccttgaatca gcaatcggat tctatgaaag gattcttggc cttaagtgcc
     3181 ataaaattga agaggtggcg gatcagaagg ttagagtggc atttttccgg atcggacaga
     3241 ccagtgttga attgcttgaa ccgacagata atgacagccc tgttgccagg tttatagaga
     3301 agagaggcga gggcatgcat catcttgcct ttgcagtgga agatattgaa aaaaaactgg
     3361 atgaggccgg caggaaggga ataaggctaa tcgataaggt tccgcgcagg ggcgcagacg
     3421 gtaaggatat tgccttcatt catccggaat caaccggcgg agtccttatc gaactctgtg
     3481 aaaaaagaaa tgggaaataa tcaataacct gaataagatg aatatggaaa agtatgaatg
     3541 tgaaatctgc ggatatattt atgaccctga gaagggggat ccggaagggg atataaaacc
     3601 gggagtacct ttcagcgatc tgccggatga ttatctttgt cctgtttgtg ctgcagggaa
     3661 ggaggaattc tcactcctgg aatagcggag gttttgcttt cagatactgt atccggattt
     3721 caagggtata ttatttatca cttccatcag aagattccag aacattggta cagttctgat
     3781 ttcaatcttc tcgtcgggag tatgtgcccc cctgatggtg ggaccgaagg agatcatatc
     3841 cattttcctg aatttctcat agatgagccc gcattccagt ccggcatgaa tggcttttac
     3901 ttccggatcc tttccgaaca gcctgcggta tgcctgacgt gtaattccga gaatctcaga
     3961 attaatatcg ggtgcccatc ccggatatcc tgctgacttt gtcacagttg ctccggcctg
     4021 tttcaggcag tcctccacca ttgccgaggc ttcggccttt gatgcttcaa aggagcttct
     4081 ctgagaggag acaatagtca cgaatccggc cggttcaaat tttaccgatg ccagattgct
     4141 tgaggtttca accaggtcct tcatgtcttc cgaccaggca ataactccgt gggggcagca
     4201 gctcagagcc ctgagcagac catcctgact gccgcggtcc ataacatgtg aaggcatggt
     4261 acattctgcc gcttccagtt tcagattcct ttcaaggtca ccaaactttt tgcggagtcc
     4321 ggtttcaatt gttttcagat gactgatgat attctctgaa ccggattttc tgcacagtat
     4381 taccgcatat gcctccctgg gtattgcatt tctgaggttg ccgccgtcaa atttactgag
     4441 ccggattccg tgttccctgt caagttcagt gagtattgct gtcattatct tgacggaatt
     4501 cccgaggcct ttgtgtatct catccccgga atgtcctccc tgcagcccgc ttaaggatat
     4561 ttccagtcca acatatcctg gcggtaccgc ttcccttgta tatctcatgg taccgaccgt
     4621 atccattccg ccggcagatc ctatgaaaag tattccctca tcctctgaat caagattcag
     4681 cagtatccgt cccctgaaaa aatcctctgt gagttccttt gcgccgctca ttcccgattc
     4741 ctcatcgacg gtaaagaggc attcaatcgt ccctgccctg agtgttttgt cggtcaggac
     4801 agccatctgg gctgcaatac cgataccgtc atcagccccc agggttgttc ccccggccct
     4861 aatccactcc ccgtctgaaa ggggtatgac gggatccttt gtccagtcgt ggggatgacc
     4921 ggcagttttt tcacctacca tgtccaggtg gctctggagc acaactattt tcctgttaag
     4981 gtgatccgga tgtgacggcc tggtgatgag gacattcccg gcgtgatcct cttttgcttc
     5041 caggttatgg tgttctgcaa actcaagcag gtactgccgt attttccctt cattccctga
     5101 cagacgcgga attctgcaaa tctcttcaaa atactgccac agggcttccg gcttcaatcc
     5161 cttgaataca ctcatattct ttttctgtga gacatcatcc cagtttcatc ttttcaactt
     5221 cctcgaggtt cctttcattg gtgatgacct ttgcgtcagg agtatagaga aaatttataa
     5281 gctcattgta tttttccatg atcttgcctc tgacaatttt cattgtcgag ttcatgagtc
     5341 cgttgtcttc ggagaaacct tcttcaagta ctccgatagc cacgggcagc catctttgcg
     5401 ggaacatttt gccgtatttc ccgtttgtcc ggtattcgtt tacctcactt tcaagcagat
     5461 tgagtatcac tctctttcct tcctcagtat cagcggtaag gtccttttcc tcgagataaa
     5521 ttttaagcgc gtgctgattg gggacgataa gtgcaacggt atagggcttc tggttgttat
     5581 agagcatgca ttggtcaatg tatttcgact gttccgaaat ggcttcctca atgccttcgg
     5641 gactgaattt ctcaccgtcg tcggcgatga gcaggctctt gaatcttccc agtacataca
     5701 ggtatccgtc ttcagtaatg tagcccatat ctccggtgta aagccagccg tcgcggattg
     5761 cttcctttgt ggctgcatca tttttccagt atccgtgcat tacattgcct cccttgatga
     5821 ctatttcacc cttttcgcct gcaggaagtt catttccgtc atcatcacat atcttaaagc
     5881 ccatgttgtt caccaggaca ccggatgatc ccagtttatg gcgtgctgct gaatttgatg
     5941 aaatgacggg agatgcttcc gacaagccgt agccctggta catcggtatt cccagtgcgt
     6001 agaaaaacct ctgaagctca atttccagca gggcacctcc tccgataaaa taatcaagtt
     6061 caccgccata tgcttcgcgg attttgctga aaatcacttt atcatataat ttcaacaggg
     6121 gttttttcag cttctgcaat ccctgccccc tgttccatcc ttccttatta taggaatatg
     6181 atatcttcag tgcatggttg aaaaggaatt cagtcagcct gcctttttcc tttatacctt
     6241 tttcaatatt cttcctgaaa ttttttgcta tggcaggtac gctcagaagg agattcggct
     6301 tgatttccct gaggcatacc ggcagattcc tgagtgtttc cagctgtgtt ctgcctgttt
     6361 ttacggatgc aatgcttgct ccccggccca tgaatgcaaa aatcccgcat gtatgaccga
     6421 aggaatggtc ccatggcagt atgagcagtg tcttgtagaa cggaggtata tccatcaggc
     6481 tgtaggactg atatacattg gtgatgtaat ttccgtgggt aaggattatt cccttgggat
     6541 cggccgtggt tccggaagta tagcagatat tggcaaaatc tgaaggcttt atacctttgc
     6601 tgatttcttc gaattttgcc acgttttccg gtgattccag ccattccctt ccaagtttcc
     6661 tgacctcgtt gaagtgaatc tcatcatctt cgtattcttc ctgtgtgtca agatgaatga
     6721 tcttttccag tgtcctgcac tcgctgatca cctgttttac cctgggatac tgcaatgtgg
     6781 agacaagcag cattgaggat tcagaatgat tcagccggaa cttaatctca tcgggattga
     6841 gtttcgttga aagcggcaca ttcagggcgc ccgtgtaaag tattccaagt tctccgatca
     6901 cccatgtatt acggccttcg gagagaaggc tgattctgtc tccttttttt acccccagag
     6961 caatcagtcc ggcagcaaat tcataaacct gtcttcttgt ctcgccatag gtggttcctt
     7021 catatttatc ctgggttttt tcccacagat aaacattgct gttgaatttc tcaacacttt
     7081 cttcaaaaaa ctgaacaatt gatttcattg attaaagggc ttacatttcg gcaaaatgag
     7141 atgtctaata tacgaattag attaaatatc ataaaatatc aaaaattatc tgaagatcct
     7201 gttctttatt aactgtttgc aatgctattc aatatttttt tatctttgat cttaaattag
     7261 tgaaagacaa tttatcaatc atcaattaat gattatgaga catccgactg caatcttact
     7321 gattttagca attgcaatga tatcatcgtg caaatttctt aaggaaaaaa ctttcctggg
     7381 caaaaaggac agattactgg ctgagtggca ggcccggcag gacagtatca gggtggcgga
     7441 ttcgataagg catgtgcatg acagtctgct ggcgatcgag aatgccaggc tggattccct
     7501 gaggctggcc gaggaagaaa agcatgctct tgaatcgaga tacaatatta ttgtaggaag
     7561 tttcatcaca cccgcgtatg ctgaagcgct ggccgatgat taccgccgca ggggctatga
     7621 cgtaagaata atacacaggg aaaacagcag gttttcgctt gtttccgccg agtcgcacga
     7681 aagtttcagg cgggctgttt cacgtattca gcagtttcag gatactgttg caatagattc
     7741 ctggatctat acccttaagt aagcggttgc ttactgattt ctgaaaattg tttccctgcg
     7801 gtccggtccg acagatacca ttgtcaccgg gaccccggtg tgatattcga taaattcaat
     7861 atagcctttc aggttcacag gcagatcgcc gaaattccgc atctccctga tttcttcctt
     7921 ccagcccggc aacacggtat agacaggctg gatttttctg ctgtcaaagg gaatttcttc
     7981 acattccctg ttttcccaca tataggaagt gcataccctg acattgtcaa atcctgacat
     8041 cacatcgctt ttcgtcatga agaggcgtgt tacgccattg atcatgattg catatttcag
     8101 agccggtatg tccagccatc cgcaccgccg cggcctgccg gtggttgaac cgaattcatt
     8161 tcccttgttc ctgagggtat ttcctgtatc atcagtcagc tcagtcggaa acgggccgct
     8221 cccgaccctg gtgcagtagg ccttgaatat tccgaacact tccccgacat gccccggcga
     8281 tatgcccagt ccggtacatg ctccggcaga aaccgtactg gatgaggtga caaaggggta
     8341 ggtgccgaaa tcgatatcaa gcattgtacc ctgggcgcct tctgcaagga ttttcctgcc
     8401 ctttaaaaca aggtcattga taaaatattc ggtgttgact atcctgaatt tcttcattat
     8461 ctccacacct tcaaaccagt ctgcctcata ttcttcagga tcaaagctga aatcatgatt
     8521 cttcaggatt gacatgtgat tcctgaagcg ctgattgtaa agctcccgga aatctgtaga
     8581 gaatatctct ccggcacgca ggccgtttct ggctatctta tcggtatagg caggcccgat
     8641 gcccttaagg gtggatccta tctttgaatc gcccctggta ttttcatatg ctgcatccag
     8701 tattctgtga cccggcagaa tcagatttgc ccttgatgaa atgaccagtc tttttacaag
     8761 ctcgtcgggc ggaatcccca gattctctgt ttccttttta aacaccgccg ggtcaattac
     8821 cacgccattt ccgataatat tgatcttgtc cggatgaaat atacctgaag gaattaaatg
     8881 taatatgtgc ttttttccat tgaactccag tgaatggccg gcattcggac caccctggaa
     8941 acgggcaacc acatcatatt gcggactgat tgcatccaca attttccctt tgccttcatc
     9001 accccattga aggcctaaca atatgtcaat cttcatgtta taaaaatatc atttccgttg
     9061 aagcagatga caagttacaa aaaatataaa acctgttacc agtcattaat ttaatgttga
     9121 taaatgataa ttcaaatcca tccggataag caggttaaaa gaaaaatgat aaccttccgg
     9181 aaaaatattt gatatatctg agataaatct ttaaatttat acgtctgttt ttctaaatat
     9241 gagtcaggtc tgaaacggtt aaaatcacaa acagggattt aatttcactt
//
LOCUS       DAOSPX010000097         9281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7133_length_9281_cov_3.737806, whole genome shotgun sequence.
ACCESSION   DAOSPX010000097 DAOSPX010000000
VERSION     DAOSPX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7133_length_9281_cov_3.737806"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLZ75_06350"
     CDS             complement(<1..399)
                     /locus_tag="PLZ75_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOS72056.1"
                     /translation="MITLTALINASCSKSSEFSADRFSVEYEKYVMANGLQVILHQDH
                     SDPMMACAIMYHVGSGREKPGRTGFAHLFEHLLFMGSENVPTGEFDRILEAAGGSNNG
                     GTTRDVTMYFEQFPKNALEKVLWLESDRMGF"
     gene            734..1459
                     /locus_tag="PLZ75_06355"
     CDS             734..1459
                     /locus_tag="PLZ75_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOS72057.1"
                     /translation="METIISKTREILGREAAVKGAGLIREALKTRGEAGIIVATGASQ
                     FDMLSVLVKDDIDWSLVTAFHLDEYIGLPITHPASFRKYLRERFADIVNPREFVYING
                     ETDPAVECKRLGDLIGRHIIDVAFVGIGENGHLAFNDPPADFETEEPYIVVNLDEACR
                     RQQLGEGWFPSFEDVPEQAISMSIKQIMKSRAVICTVPDLRKAEAVRNTLKEPVSPLV
                     PATILRNHGNAWLFLDQDSASLL"
     gene            1509..1724
                     /locus_tag="PLZ75_06360"
     CDS             1509..1724
                     /locus_tag="PLZ75_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HOS72058.1"
                     /translation="MKKDLNKMKTSKLAFSVSIGLVIGAAIGVIIGAATGELALWLAI
                     GIGCGIALGAGVGGTLEGKDRKKRETE"
     gene            1766..2242
                     /locus_tag="PLZ75_06365"
     CDS             1766..2242
                     /locus_tag="PLZ75_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS72059.1"
                     /translation="MKYRIRKATEEDFPAVLSLIKELAEFEKAPEQVTNSVEQMKREK
                     DYFLCYVAETDEKEIIGMALFYFVYYTWVGKSLYLDDIYIRKDFRKNRVGSALLRKVF
                     EVAKAEDCRRIRWQVLNWNEPAIEMYRSIGALIEDEWLNCTFDAEGISNFLKTRSP"
     gene            2296..3252
                     /locus_tag="PLZ75_06370"
     CDS             2296..3252
                     /locus_tag="PLZ75_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HOS72060.1"
                     /translation="MNKLRISTAQFENRSGDKAYNLGIIGDLAREAAGEGSSIIAFHE
                     CSVTGYTFARNLSRDQLLGLAEYIPEGDTIRKLTRIAAENEIVVLAGLFEKDRDDRIY
                     NSYVCVDRNGLVARHRKLHPFISRYLSPGGSYTVFDLLGWKCGILICYDNNIAENVRA
                     TKLLGADILFMPHVTMCTPSTRPGAGFVDPELWYNRENDPTSLRLEFDGMKGRAWLMK
                     WLPSRAYDNAAYVVFANPLGMDDDQLKNGCSMIIDPFGDILAECRSFHDSFATASIIP
                     EKLTQAGGYRYLEARRPELYRDIIGRAHDPEQKVSWLGKPGH"
     gene            3281..3571
                     /locus_tag="PLZ75_06375"
     CDS             3281..3571
                     /locus_tag="PLZ75_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72061.1"
                     /translation="MKNDFCEALKTNDRRRLQELADSVLGCLDLQECQQLNFKKIEIW
                     ISSNNCVASVSASPYLLDTDPPVKEFILNLRDGSVRTLGLRLSPSKWEIIIK"
     gene            3581..5128
                     /locus_tag="PLZ75_06380"
     CDS             3581..5128
                     /locus_tag="PLZ75_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72062.1"
                     /translation="MERKDFVRKQKMMEASLRDVKTALSGISGVVAVGIGLKESGRKF
                     TEEISYRVYVPHKKHRTELRPEEIIPPSIDGIPTDVLTPYSIVNDSGVCGDERMTLSL
                     KRPLRAGIAVSGDSVSYGTLGWFGKLHDGTDVLLTNKHVLYDDTNQTDLRKLKTGQPR
                     LGDPAACFCYACGNDNLIGESLIGIRDIHPPSDTSVDCAIARINPELARDIVLRITND
                     STEEVLSVSGTASAVIGDKVRKIGARSGYTRGTVIHIGDIAVSVPPDSDETAVAVRRG
                     QVLIIPDPEESYQVREGGVCKPAFTNSGDSGAVILNQDNRIIALNWGGDRTTGSVGIT
                     IACKIQNVLDKLSSAGYKVTLSVSPPAGDNDFPEKEKTGFSADPSGLNILGKLRDSNK
                     KSLLYWLYERHNREIMSLITGSRPVGVAWQRNQGPAYVAAIVSASGDKNFQIPFAINS
                     VSREALLKILGKVLTEHGSDSLKADIKRYRKDIMKIADQGRTIEELALILKKAGLIDE
                     IPSDVFS"
     gene            5397..6623
                     /locus_tag="PLZ75_06385"
     CDS             5397..6623
                     /locus_tag="PLZ75_06385"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS72063.1"
                     /translation="MNKELKKRVTIAALIVVCLGLVIFVLIRFLLPFSVKPGDYVISN
                     VDFGPVYTTVPASGIINPENEVLLLSPSSSVISSLSRAPGSRVSKGDIILTLDSKSLM
                     QEIDNLKDQLEVMENDLQKNRLNSRSTRVDLNYNAEVKRLKITSLKTEIADQEQLLKV
                     GGISPAFHEQTKEELVLAEKDLNMTLEKNSIRLKQLEADEKGLQLQIDMRNKELVLRQ
                     TLLERLNIRAPSDGIILGIYTNVGERVEKDKLLVKMSDLSTFKIKATIDNPYVNLVKT
                     GGEVYALVNDISLRGKIGTVSPVMRDKKIEFDVFLDHSHYDKLIPNMEVALQIVTQKK
                     DSVLRVGYGPVFSRAKSQELFVLNTYKAVRKTVTTGLIGTDHVEITEGLNPGDRVIIS
                     NVHLSRHIREFELMDL"
     gene            6637..8133
                     /locus_tag="PLZ75_06390"
     CDS             6637..8133
                     /locus_tag="PLZ75_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS72064.1"
                     /translation="MKINLRYSFLVLFFSVLIHPVSGDSTDTVSFTLSLRNVVDLAII
                     QSASIKYAQNTNVNYFWRWKNFQTSFRPQLILTGEVPNFTQQNIPVVQPDGSIQFRKI
                     SQLQGSSQLALSQSIPGTGTSVYAATNLYRVQDFIKHSVEYSGSPFQIGLTQPIFAYN
                     NMKWQKKTEPLIYEESRKQFVESTERIALQATIYFFRYLMIQTNYNLAENNLKNSNNN
                     LRIAEVRKQLGKISDNDFARIQLSVFNAQKALNKASMDLKNADFELKSYINLDQHQKI
                     ELVIPVEMYLFSVDPGKALEEAKENRKETPYFERRLIEAERDLRRARRTSGLSATVVG
                     SFGLSNSSETYGGVYEQPEKQRVISLRMSIPVLDWGRSASNIKLAESRRELVLYDVEK
                     EIKDFEREVVVQVEQFGLLRDQITTAQEADKVAENGYLIALKKFQSGEISITDLNIAL
                     SERESAKRDYITAIESYWQSYYRLRILTLYDFELNQKIMYDNPMLNSE"
     gene            complement(8234..8325)
                     /locus_tag="PLZ75_06395"
     tRNA            complement(8234..8325)
                     /locus_tag="PLZ75_06395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8289..8291),aa:Ser,seq:tga)
     gene            8644..8913
                     /locus_tag="PLZ75_06400"
     CDS             8644..8913
                     /locus_tag="PLZ75_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOS72065.1"
                     /translation="MTKKELVASIAKESGLTVKDSTKALNAFVASVGKAMKKGERLQL
                     PGLGTFKVTKRSARVVRNPRTGAKLNVPAKKVVKFKAAPALDKGL"
BASE COUNT         2671 a         1884 c         2270 g         2456 t
ORIGIN      
        1 gaatcccatt ctgtccgatt ccagccagag gactttctca agtgcatttt tcggaaattg
       61 ctcaaaatac attgttacat ccctggttgt gccgccgttg tttgatccgc ctgcagcttc
      121 cagtatcctg tcaaactcac ctgtcggcac attttctgat cccatgaaaa gcagatgctc
      181 aaaaaggtgt gcaaaacctg ttctgcccgg tttctccctg cctgatccca catggtacat
      241 tattgcgcaa gccatcatag gatcggaatg atcctggtgc agtatgacct ggagaccgtt
      301 ggccatcacg tatttttcat attccacgct gaacctgtcc gcggaaaatt cagaggactt
      361 actgcatgat gcattgatga gtgctgttaa tgttatcatt aaaatgactg taaatgaata
      421 aaagcttgtt ttcattgaaa taaaatgatt gtattaaaac gcggataaac ggaactgcga
      481 aaatataaaa ttatttgcag tcatctgctt tttcagggaa caggaacaat tgtaaatttt
      541 tatgtatatt tccctttcaa aaaacaggag agtgtaaaaa accagttccg gcatttccgg
      601 caagctgcag cggcagcaat atttataccc gtataccggc tggcccccga tcatattagg
      661 ttataatatt atgattccgt gatcgttgat atgcattaat gataatcaga caaacaataa
      721 ataacagcat taaatggaaa caataatttc aaaaacccgt gaaatcctgg gaagagaggc
      781 agcagttaaa ggtgccggac ttattcgtga agcgttaaaa acaagaggtg aggccggtat
      841 tatagtggca accggtgcat cccagttcga catgctcagt gtgcttgtaa aggatgacat
      901 tgactggtcg ctggtcaccg ctttccatct ggatgaatat attggccttc cgataaccca
      961 tccggcctca ttcaggaaat atctcaggga aagattcgcc gacatagtca acccccggga
     1021 atttgtttac atcaatgggg aaactgatcc ggccgtagaa tgtaagaggc tgggagacct
     1081 gataggcaga cacattattg atgttgcatt tgtgggaata ggcgaaaacg gacatcttgc
     1141 gttcaatgat cctcctgccg atttcgagac tgaagaaccc tatattgtag tgaatcttga
     1201 tgaggcctgc cgccgtcagc agctcgggga aggatggttt ccctcctttg aagatgtgcc
     1261 tgaacaggcc atcagcatgt ccataaaaca gatcatgaaa tccagggcag taatctgcac
     1321 tgttccagat ctgcggaaag ctgaagcagt cagaaacaca ctgaaggagc ctgtttctcc
     1381 ccttgttcct gccactattc tgcggaatca tgggaatgcc tggcttttcc ttgaccagga
     1441 ttcggcttca ttgttataat tagatgatct ggtacatgcc agtctgatgc ctgtatatat
     1501 atcaggccat gaaaaaggat ttgaataaaa tgaaaaccag caagcttgct ttcagtgtgt
     1561 caatcgggct ggttatcggt gcggcaatag gagtgattat tggtgcagcc accggtgaac
     1621 tggcattgtg gctggcaatc ggaatcggtt gcggaattgc actgggcgcc ggggttggag
     1681 gcaccctgga aggaaaagac aggaaaaaga gggagacaga atagggcatg ccggaattat
     1741 atttgcctga attatttatg cagaaatgaa atacagaata agaaaagcaa ctgaagagga
     1801 ttttccggct gttctttcgc ttataaaaga actggctgaa tttgaaaaag ctccggaaca
     1861 ggtgactaat tcagtggaac agatgaagag ggaaaaggat tatttcctgt gttatgttgc
     1921 tgaaaccgat gagaaagaaa taataggaat ggcgctcttc tattttgtgt attatacctg
     1981 ggttggcaaa tccctgtacc ttgatgatat ttacatcagg aaggacttca ggaaaaacag
     2041 ggtgggaagc gcgctcctga ggaaagtatt tgaagtagcg aaagctgagg actgcaggag
     2101 gatccggtgg caggtactga actggaatga accggcaata gagatgtaca ggagtatagg
     2161 cgcactgatt gaggatgaat ggctgaactg tacttttgat gcagaaggga tcagtaattt
     2221 tctgaaaacc cgttctccgt aaacacaggg aatggcaggt tgtttaattt gtccggctgg
     2281 tttaaaaaga acaatatgaa caagttgcgc atatcaacag cacagtttga gaacagaagc
     2341 ggcgacaagg cctataatct ggggataata ggggatctgg cgcgggaagc tgcaggggaa
     2401 ggctccagta tcattgcatt tcatgaatgt tcggttacag gatacacctt tgcccggaat
     2461 ctttccaggg accagctgct cggtcttgcg gaatatattc cggaagggga tacaatccgt
     2521 aagcttactc ggatagcagc tgaaaatgag attgttgttc ttgccggact ctttgaaaag
     2581 gaccgtgatg acaggatcta caacagctat gtatgtgttg acaggaacgg gctggtggcg
     2641 cggcacagga agctgcatcc ctttataagc cggtatctga gtccgggggg ctcgtatacc
     2701 gtttttgatc tcctgggatg gaaatgcggc attctgatat gttatgacaa caatatcgct
     2761 gagaatgtca gggccacgaa actgctaggt gccgatatac tgttcatgcc tcacgtgacg
     2821 atgtgcactc cctccacccg tccgggagcg ggttttgtgg atccggagct gtggtataac
     2881 cgggaaaatg atcccacatc cctcaggctg gagtttgacg ggatgaaagg cagggcatgg
     2941 ttaatgaaat ggcttccttc acgggcctat gacaatgctg cctatgtggt ctttgcaaac
     3001 ccgcttggca tggatgatga ccagcttaag aacggctgtt ccatgataat tgatcccttc
     3061 ggggatattc ttgcagaatg ccgttcgttt catgacagtt ttgcaactgc aagcattatt
     3121 cctgaaaagc tcactcaggc cggcggatac cgctatcttg aagcccggcg gccggaactc
     3181 tacagggata taatagggag ggcccatgat cccgaacaaa aagtgagctg gctcggcaag
     3241 cccgggcact gagttgacag cttgtttaaa ccgggcaaat atgaaaaacg atttttgtga
     3301 agccctgaag accaacgacc ggagacgcct gcaggaattg gctgacagtg tccttggctg
     3361 cctggattta caggagtgtc agcaactgaa ttttaagaaa atagaaatat ggatcagcag
     3421 taataattgt gtagcttccg tatctgccag cccctatctc cttgataccg atcctcccgt
     3481 caaggagttt atcctgaatc tcagggatgg ctcggtcagg actttgggtc tgaggttgtc
     3541 tccctcaaaa tgggaaataa tcatcaaata atttattgta atggaaagaa aggatttcgt
     3601 acggaaacag aagatgatgg aggcctcgct ccgggatgtg aaaactgctt tgtccggcat
     3661 atccggcgtg gtggctgtcg gtatcggact gaaggaatcc ggcaggaaat ttactgaaga
     3721 gatttcttac cgggtctatg tgcctcataa gaagcatcgt acggaactac ggcccgagga
     3781 aataataccg ccctcaattg acggtattcc caccgatgtt cttacaccat attccattgt
     3841 aaatgattcc ggagtgtgcg gggatgagcg gatgacactg agtctgaaac gtccgctcag
     3901 agcaggaata gccgtatcgg gtgattcagt cagttacggg acacttggct ggttcggcaa
     3961 actgcatgac gggactgatg ttttgcttac caataagcat gtattgtatg atgataccaa
     4021 tcagaccgat ctgagaaaac taaaaaccgg ccagcccagg cttggtgatc ccgctgcctg
     4081 tttttgctat gcatgcggga atgataatct gatcggggaa tcccttatag ggatccgtga
     4141 tatccatcct ccctccgaca catcggtgga ttgtgccatc gccaggatta atccggaact
     4201 ggcccgggat attgttctga gaattacaaa tgattcaact gaggaggtat tgtcagtgtc
     4261 aggtactgcc tcagccgtga tcggtgacaa agtgaggaag ataggagccc gctcgggtta
     4321 taccaggggc acggtaatac atatcggtga cattgctgtt tctgttccgc ctgattcaga
     4381 cgaaacagct gttgcagtcc gccgggggca ggtactcatc atacctgatc ctgaagagag
     4441 ctaccaggtg agagaaggag gtgtctgcaa gccggctttt accaacagcg gcgattcagg
     4501 agctgttata ctcaatcagg ataacagaat cattgcgctt aactggggcg gtgacaggac
     4561 taccggcagc gtgggaataa caatagcctg taaaatccag aatgtactgg acaaattaag
     4621 cagtgccggc tataaagtca ccctttccgt cagtccgccg gcgggagaca atgattttcc
     4681 ggaaaaagaa aaaaccggct tttcagctga cccgtccggt ctgaacatac tcggcaaact
     4741 ccgcgattcc aataaaaaaa gccttctgta ctggctgtat gaaaggcata accgggaaat
     4801 catgtctttg atcacgggct cccgcccggt tggtgttgca tggcaacgaa accagggacc
     4861 ggcctatgtt gctgcaattg tcagtgcgtc aggcgataag aatttccaga ttccttttgc
     4921 aatcaattca gtatcccgtg aggcattgct gaagattctc gggaaggtat tgacggaaca
     4981 tggaagtgat tccttaaaag cggatataaa gcgataccgg aaagatatca tgaaaattgc
     5041 ggatcaggga agaaccattg aggaactggc gctaattctt aagaaagccg gactgattga
     5101 tgaaattcct tcagatgtgt ttagttgatt tttccggata atccgtttta aattccaaat
     5161 tactgttaaa attcgtttgt tcttccgcga aaaatttata tttgggattt ttgtctcaat
     5221 aagccggaaa tggtataaat ggtgccgggg tcagggtatg ttcggttatt cccctgttcc
     5281 tgccggatca tattatcttt acggcagccg gatgcatgaa tatgaaacaa atcccggaag
     5341 aatcaggctg tatcctgtat tgaaatttat attatttaca tgttaaatac tctcatatga
     5401 ataaagagct taaaaaaagg gtaacgattg ctgctctcat tgttgtctgc ctgggcctgg
     5461 tcatttttgt actgataagg tttctgctgc cattttccgt gaagcccggg gattatgtga
     5521 tatcaaatgt ggattttggt ccggtttata caacagtgcc ggccagcggg attattaatc
     5581 ctgaaaatga ggtattgctg ctcagcccgt catcaagtgt tatatcttcc ctttccaggg
     5641 cacctggaag cagggtatcg aagggtgata taatattgac gctggattca aaatccctta
     5701 tgcaggaaat tgataatctg aaggaccagc ttgaagtgat ggagaatgat ctgcagaaga
     5761 acaggctcaa ctcgaggagc acacgtgttg atcttaacta taatgcagaa gttaaacgcc
     5821 tgaaaatcac ttctctcaag acagaaatag cagatcagga gcagcttctg aaggttgggg
     5881 gaatctctcc ggcttttcat gaacaaacca aggaagaact ggtactggcg gaaaaagacc
     5941 ttaacatgac acttgaaaaa aattcaatac gcctgaaaca gcttgaagct gatgaaaaag
     6001 gacttcagct ccagattgac atgagaaaca aggagctggt tttaaggcag acccttctgg
     6061 aaaggcttaa catcagggcc ccgtctgacg ggataatact ggggatttat accaatgtgg
     6121 gcgaaagggt tgaaaaggat aaactgctgg tcaaaatgtc ggatctgtca acattcaaga
     6181 tcaaggcaac aatagataat ccctatgtaa acctggtcaa aaccggcggg gaagtgtatg
     6241 cactcgtcaa tgacatcagc ctcagaggaa agattggcac cgtatctccc gtaatgaggg
     6301 acaagaagat agaatttgat gtatttctcg atcacagtca ttatgacaag ctgattccca
     6361 acatggaagt tgcactgcag attgtgaccc agaaaaagga cagcgtgttg cgggttggat
     6421 acggcccggt tttcagcagg gcaaaaagcc aggaactgtt tgtacttaat acctataaag
     6481 ctgtcaggaa aacggttaca accggactga tagggacgga ccatgttgaa ataaccgaag
     6541 gccttaatcc cggtgacagg gttattattt ccaatgtcca tctttccagg catatacgtg
     6601 aatttgaact tatggattta taaatcggat tgttatatga aaattaattt aagatatagt
     6661 tttcttgttt tatttttttc agtcctgatt catcctgttt caggtgattc cactgatact
     6721 gtttcattta cactgagttt gagaaatgtt gtcgatctgg caataataca atcagcttca
     6781 ataaaatatg cccagaatac aaacgttaat tatttctggc gctggaaaaa tttccagacc
     6841 agcttcaggc cccagcttat tctcacgggt gaagtgccga attttaccca gcaaaatata
     6901 ccggtggttc agcctgacgg cagcattcag ttcaggaaga tatcccagct ccagggttca
     6961 tcgcagcttg ccctgagcca gtctattccg ggaaccggaa catctgtcta tgccgcaaca
     7021 aacctttacc gtgttcagga tttcatcaag cactctgtgg aatattccgg cagccctttc
     7081 cagatcggtt taacccagcc aatatttgcc tataacaata tgaaatggca gaaaaagact
     7141 gagccgctga tatatgagga atccaggaaa caatttgttg aatcaactga gaggattgct
     7201 cttcaggcta ccatatactt tttcaggtat ctgatgattc agaccaacta taacctggcg
     7261 gaaaacaatc tgaaaaacag taataacaat ctaaggattg cggaagtaag gaagcagctt
     7321 ggcaagattt ccgataatga ttttgcgcgg atacaactgt ctgttttcaa tgcacagaaa
     7381 gcattgaata aagccagtat ggatcttaaa aatgccgatt ttgagttaaa atcctatatt
     7441 aacctggatc agcaccagaa gattgagctg gtaataccgg ttgaaatgta tcttttctca
     7501 gtagatccgg gaaaggccct ggaagaggca aaggaaaacc gcaaggagac cccctatttt
     7561 gaacgaaggc ttattgaagc agaaagggat ctccggaggg ccaggagaac ttcaggctta
     7621 agtgccacgg tggtcgggag ctttggcctg tccaacagtt ccgaaactta tgggggtgtc
     7681 tatgagcagc cggaaaaaca gagagtgatc agtctcagga tgagcatacc ggtccttgac
     7741 tggggccggt ccgcgtcaaa tatcaagctg gccgaatcaa ggcgggagct tgttttgtat
     7801 gatgtggaaa aagaaatcaa ggattttgaa agggaagtgg ttgtacaggt tgagcagttc
     7861 ggactgctgc gcgaccagat tacaacggca caggaagcgg ataaggttgc tgagaatggc
     7921 tatctcattg ctctcaagaa attccagagt ggtgaaatca gcattaccga cctgaatatt
     7981 gcactttctg aacgcgaaag cgccaaacgg gactatatca cagcaattga gagttactgg
     8041 caatcttatt atcgtttaag gatacttaca ctttatgatt ttgagctgaa ccagaagata
     8101 atgtatgata atccgatgct gaattcagag taaacggttt atttaccttt tacgccggtg
     8161 cacttcgttt atcaccggtc ggttctcacg aaaaaacccc gcagtctgta ctgcggggct
     8221 gatctgacaa gtctggcgga gaaagaggga ttcgaacccc cgttccgccg gcgggcggaa
     8281 aacggttttc aagaccgccg cattcgacca ctctgccatt tctccgttgc aaatataatg
     8341 atttttattc ttcatttcct tttaaaaaaa agaaaacctt ataaaaggca ctatatcagg
     8401 tatatataaa atttatgaac agattgtcaa caagttaagg tgtgaaatgt ctttaaatga
     8461 ccatataatg gccgctaacg taaattttaa caaaatttgt atattttgga aaaaagcgca
     8521 tttttgtgtc gtaaagtccc actaaatgtg caaagaaggc aagaattaat ttgcatatga
     8581 atttaaatga gataaatttg tattcaatta gtaggattgg tctaaccttt aatttaaatt
     8641 actatgacaa agaaagaatt ggttgcttcc atcgcaaaag agtcaggcct tactgttaag
     8701 gattcgacaa aagccctgaa tgcatttgtg gcttcagttg gcaaagccat gaagaagggc
     8761 gagaggcttc agcttcccgg tcttggcaca ttcaaggtaa caaagagaag tgcacgggtg
     8821 gtccgcaatc cgcgtaccgg tgccaagctg aatgttcctg caaagaaggt ggtgaaattc
     8881 aaagcagccc ccgctcttga caaaggtctt taatcgtaaa tgattgaaaa tgcctggagg
     8941 gtgtccgtga cacccttttt tttgtattta attaatactg actgattagc aataaggctc
     9001 cggcttcctg aagatatgaa caaaatcagt taaattatgt tttttttctc cagttacagt
     9061 tttttttatt acttttgatt tgtctaaaca tttctgacta gtaaagtatg aattgtatta
     9121 ttgttgatga cgatcagctt agttgtaaaa ttctcgaagg ctatgttgcc aagtcttcgt
     9181 ccctgaatct ggttggtacg ttcaacgatt ccgtggaggc cagaaatgtt attaccaaaa
     9241 ggcaggatat cgacctggta attcttgaca tcaagatgcc c
//
LOCUS       DAOSPX010000098         9246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7159_length_9246_cov_3.867044, whole genome shotgun sequence.
ACCESSION   DAOSPX010000098 DAOSPX010000000
VERSION     DAOSPX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7159_length_9246_cov_3.867044"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..315
                     /locus_tag="PLZ75_06405"
     CDS             169..315
                     /locus_tag="PLZ75_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72066.1"
                     /translation="MNRIKVVLEDKGIKQTLLADKPGKNYNLVNEYVQNRQSERGKGI
                     KFDP"
     gene            399..1541
                     /locus_tag="PLZ75_06410"
     CDS             399..1541
                     /locus_tag="PLZ75_06410"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOS72067.1"
                     /translation="MKESIHINHEVLVWARESLALNQAQASESTGISPKRLVQLESGG
                     KQPTLAELKAFSKTYKRTIATLLLNNPPKEKPFPADRRSIDSKELGYFHEKTIMAVRK
                     ARALAQSFVELREEMGMPFPKFNLSESLKNNPREVAEKIRQLLHLDEIREIEHVRIAL
                     EVYIERIENLGIAVFQLSLTQDNVRGFAIVDDLIPIIGIKRGGEQDHSKTFTLFHELG
                     HILLNENAISDLSLNPQWEVEKWCNAFAAEVLVPSEELLRMDIVRSYQEQGQEEWLLK
                     DLTELGRHFHVGPLAILRSLFEQKLTTAAFYKEKHKQWNKPPFARAKNSEGRDLAKEA
                     VQEKGKTYISLAFMAFDQNRINIKDLSDFLGVKLSYIPKARQFLNA"
     gene            1544..2062
                     /locus_tag="PLZ75_06415"
     CDS             1544..2062
                     /locus_tag="PLZ75_06415"
                     /inference="COORDINATES: protein motif:HMM:NF025725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HOS72068.1"
                     /translation="MDLFSEGNKVYVIDTSALMRLDFNFKRGNPVFTAIWEEIEDLIR
                     QGCFKTIDFVEQEINEYRGKEVFLKDWIKKWRKLLVVDTDVASFNAAKPIINEEYNTG
                     FLTPNKQAEGKEEADPYLIGYCKVHNYVLITDENKLKPNRIPAVAHKNGVKCIDIYEF
                     LQERGLKMERKR"
     gene            complement(2076..4022)
                     /gene="dnaG"
                     /locus_tag="PLZ75_06420"
     CDS             complement(2076..4022)
                     /gene="dnaG"
                     /locus_tag="PLZ75_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOS72069.1"
                     /translation="MIDRSTIERILNAAQIVEVVQEFVPLKRRGANYLGLCPFHNEKT
                     PSFTVSPSREIFKCFGCGKVGNVVNFIMEHEHLSYPEALKFLARKYHIEVVERELSQE
                     EIEKQNERESLLVVTAYASRQFSENLFNSDEGRSVGLSYFRERGFRHNTLKKFETGYS
                     LEKRDAFSQKALDDGHRKDFLVKTGLSIEHEDRIFDRFSGRIIFPIHSLSGQVLGFGG
                     RILKTDAKAAKYLNSPESEIYHKSRILYGIYQARRAITKEDKCFLVEGYTDVMSLHEA
                     NVENVVASSGTSLTREQVRLIKRFTSNITMLYDGDPAGIKASLRGTDLVLEEGMNVRI
                     VLLPENEDPDSWSRKVSNEEFRNYLNENETDFIRFKINLLIEDAAGDPVKKANLIRDI
                     VRSIAVIPESITRTIYIKECSVVMEVPEEVLYHEVNKMRQQRSFQDRNKYPGPEDLPV
                     PPPVIPKKAKQEVTTYVSEKEIIRILLKFGSNEFDRVIRKEDGREEVTTVADYIVREI
                     VSDELTFDDMILGNIFEEFRFASEQGVFLEDKVFIKNEDPAISMLSADILAESHQLSR
                     IWVNKHTYVETEDMKLREIVPDAVLKFKGDKIKLRLQELMEELREAVSSGDMERIPEL
                     QKRDQALKAALGIISRQLGNRILL"
     gene            complement(4035..4733)
                     /locus_tag="PLZ75_06425"
     CDS             complement(4035..4733)
                     /locus_tag="PLZ75_06425"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOS72070.1"
                     /translation="MKKLVILTGAGMSAESGIRTFRETGGLWEEYDVNEVASPMGWAK
                     NRDLVLRFYNERRTQLAVCEPNDGHKGLAALEKYFDVHVITQNVDDLHERAGSTKILH
                     LHGELTKVRSTKDPSLVYDIGYKEVNPGDLCEKGSQLRPHIVWFGEAVPLMDEAVEIT
                     STADIFAVIGSSLNVYPAAGLVGFAPRNASLWLIDPHDVYVPSVKNINVIKEKASDGV
                     RILTRNLLEQYGII"
     gene            4954..5415
                     /locus_tag="PLZ75_06430"
     CDS             4954..5415
                     /locus_tag="PLZ75_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOS72071.1"
                     /translation="MLHRYRIKNKYGIRSLAFSIFLFLAPVLAAAARDPDSLKFKSLD
                     PHYFHLQYLKENPAWLIDVRMSFEYRGRRIRDAINIPSAKELYAFTDTISKNTALFLY
                     CTDGFRSKQAARLLYDKGFRNLFNLEGGIIAWKKDGFPLEKGRVKIKKRKR"
     gene            5498..6097
                     /locus_tag="PLZ75_06435"
     CDS             5498..6097
                     /locus_tag="PLZ75_06435"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOS72072.1"
                     /translation="MFSGIVEEAAPVVKLEREQDNLHIIMKCSFTHELRIDQSISHNG
                     VCLTVVKTEGDCYTVTAIKETLEKSNLGLLKTGDKVNLERSTRLDARLDGHMVQGHVD
                     QTAVCTEVTEAGGSWYYTFEYEPLQDDYLTVEKGSVAVNGVSLTVVNSQPKSFQVAII
                     PFTWEVTNFHQIKAGTVVNIEFDILGKYIAKIVRQQLQK"
     gene            complement(6161..6616)
                     /locus_tag="PLZ75_06440"
     CDS             complement(6161..6616)
                     /locus_tag="PLZ75_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOS72073.1"
                     /translation="MVDRRIKRFFRKHHVLTIATSVRETPWCASCFYVYLEEENSLVF
                     TTDLKTRHGQEFLKNASVAGTVALETGIIGRIRGIQFSGTVYEPEGEMAARAESAYLK
                     RFPVAMLMDTRLWVVKISYLKYTDNRLGFGKKLIWDLSSGSGQSSEFHF"
     gene            complement(6609..7184)
                     /locus_tag="PLZ75_06445"
     CDS             complement(6609..7184)
                     /locus_tag="PLZ75_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOS72074.1"
                     /translation="MEKSERKIILAVTGASGSVYALKLLDFLRKIKDPPAETAVIFSD
                     NGKEIWKYENGRDYEAFPPEKEYDSRDFHAPFASGSSDYDTMIICPASMGILGRIASG
                     SSDDLIARAADVMLKEKRKLIVVPRETPYNLVHVRNMETILLAGGIVCPASPSFYSRP
                     GNIDDLVMTVVERIVSLAGFDAPLHRWMKNG"
     gene            7294..>9246
                     /gene="ileS"
                     /locus_tag="PLZ75_06450"
     CDS             7294..>9246
                     /gene="ileS"
                     /locus_tag="PLZ75_06450"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOS72075.1"
                     /translation="MRGKFPEYKTLDLSRVNKDILKIWDRDNIFFKSIRQLNGKGEYI
                     FYEGPPSANGMPGIHHVLSRTIKDAICRYRTQCGYEVKRKAGWDTHGLPIELGVEQAL
                     GITKEDIGTEKISIEEFNRACRIDVMKYTDQWEDLTHRMGYWVNMDDPYITYDNRYIE
                     TLWWLLKQLYSKGYLYKDYTIQPYSPAAGTGLSSHELNLPNCYRDVRDTTCIALFRLI
                     RNEKSEFLFEDIDTADVHMMAWTTTPWTLPSNTALAVGKDIEYIKVRSFNPYSGEPIT
                     VIFAGDLRDAMFPGTDESADLPEYRAGDRKVPFRVLDRFPGSRLEGIDYEQLIDWVKP
                     GEGAFKVLTGDFVTTEDGTGIVHIAPTFGADDYKIAREYGVPAMLLRMKDGTFGPMVD
                     KKGYMVPVSDLDEKFVRERVSLNSYGPFEGRPVKNEYDENIDPDTDTLDVDIAVMLKQ
                     TGKVFRIEKMEHSYPHCWRTDKPVLYYPIDAWFIRTTAFRDRLIELNNTINWKPASTG
                     SGRFGKWLENLVDWNLSRSRFWGTPLPVWRTDDGKESVCIGSVSELKAEMEKSVMAGF
                     MESNPLATFTEGDNSPENYNKLDLHRPFVDNIILVSPSGRKMFREPDLIDVWFDSGAM
                     PYAQSHYPFENRDNFSEMFPADFVAEG"
BASE COUNT         2580 a         2049 c         2146 g         2471 t
ORIGIN      
        1 caggccttaa taaagggtac tggctaccac gggccttcgt aaaacccaaa gagcattttt
       61 atcaacagcg tgaaggatag tgaaacaata attcaaacac ttcttatatt gaaatatgag
      121 ataagtcaat aattaagaac tattttagca gtttgaatca ttgtccgcat gaacagaata
      181 aaagtagtac tggaagataa aggaataaaa cagactttgc tggctgacaa acccggtaag
      241 aactacaacc tggtaaatga gtacgttcaa aaccggcaat cagaacgtgg aaaaggtatt
      301 aaatttgatc cgtaaaggaa gattatctgt tttaaaagca caaccaaata tttgagaatt
      361 gccttcttaa tttgttacaa tatttaatca aagtttacat gaaggaaagt attcacatca
      421 atcatgaagt ccttgtatgg gcgagggaat cgcttgcact gaaccaggca caggcttctg
      481 aaagtactgg tatttcgcct aagcggttgg ttcaattaga atcaggtggg aaacagccca
      541 ccctggcaga gcttaaggca ttttcaaaaa catacaaacg aactattgct acacttttac
      601 ttaataatcc accaaaggaa aaaccgtttc ctgcagatag acgtagcatt gattcaaagg
      661 aacttgggta ttttcatgaa aaaaccatta tggcagtccg taaggcaaga gctttggctc
      721 aatcgtttgt tgagttgcgc gaagaaatgg gaatgccatt tcccaaattc aatttatctg
      781 aatcattaaa gaataatccg cgggaagttg cagaaaaaat tcgtcaactg cttcacttgg
      841 acgaaataag ggaaattgaa catgttagaa tagcattgga agtttacata gaacgcattg
      901 agaacctggg tattgctgta tttcaactaa gcttaacaca agacaatgta cggggttttg
      961 ccattgtaga tgatcttatt ccgattatcg gcataaaaag aggaggcgaa caggatcact
     1021 caaaaacatt taccctattt catgaactgg gacacatttt attaaatgag aatgcgataa
     1081 gtgatttatc tctgaatccg caatgggaag tggaaaagtg gtgtaacgct tttgctgccg
     1141 aagtattagt gccttcagaa gaattattgc gtatggatat tgttcgctca tatcaggagc
     1201 aaggtcagga agaatggtta ttaaaggacc tgactgagct tggcaggcat tttcatgttg
     1261 gccctctggc aatactgaga agcttatttg aacagaagct taccacagca gctttttata
     1321 aagaaaaaca caaacaatgg aacaagccgc ccttcgcaag ggcgaaaaat tctgaaggga
     1381 gagatttagc caaagaggcg gtccaggaaa aaggcaaaac ctatattagc ctggcgttca
     1441 tggcatttga ccaaaatcgc attaatatta aagatttgtc tgattttctg ggagttaagt
     1501 tgtcctatat tcccaaggcc cgtcaatttc tgaacgctta acaatggact tattcagtga
     1561 aggaaataag gtttatgtaa ttgacacaag tgccctgatg cgattggact ttaattttaa
     1621 aaggggaaat cctgtattta ctgcaatttg ggaggaaatt gaagatttaa tacggcaggg
     1681 ctgcttcaaa accattgact ttgtcgaaca ggaaattaat gagtataggg ggaaggaagt
     1741 atttctaaaa gactggatta agaaatggag aaaactatta gtagttgata cagatgttgc
     1801 aagtttcaat gcagcaaaac ctattatcaa tgaagaatac aatacggggt ttcttacacc
     1861 aaataaacaa gctgaaggca aagaggaggc agacccatat ttgattggtt attgtaaagt
     1921 acacaactat gttttaataa cagacgaaaa taaattaaag ccgaatcgaa ttccggcagt
     1981 agcacataag aatggagtaa aatgtataga tatatatgag tttttgcaag aaagaggatt
     2041 aaaaatggaa aggaaaagat aaaaaatgtt tgtatttaca gcagtatcct gtttccgagt
     2101 tgccgggata ttattccaag tgcggccttg agtgcctggt ctcttttctg gagttccgga
     2161 atcctttcca tatcgcctga gctgactgcc tcccggagct cttccattaa ctcctgcagt
     2221 ctgagcttta tcttgtcgcc cttgaatttc aggacagcat ccggaacaat ttccctcagc
     2281 ttcatgtcct cggtctcgac ataggtatgc ttgtttaccc atatcctgct cagctgatgt
     2341 gattcggcaa gaatatccgc ggacagcatt gaaatggcag ggtcttcatt tttgataaag
     2401 accttgtctt caagaaatac tccctgttcg cttgcaaacc tgaattcttc aaatatgttt
     2461 ccgaggatca tgtcatcaaa ggtcagttca tccgaaacaa tttcccttac tatataatcg
     2521 gccacggttg taacttcctc ccgtccgtct tccttacgga tcaccctgtc aaactcattg
     2581 cttccgaatt tgaggagaat cctgattatt tccttttcgg atacgtaggt tgttacttcc
     2641 tgttttgcct ttttgggaat gaccggcggc ggaacgggca gatcctctgg tccgggatat
     2701 ttgttcctgt cctgaaatga tctctgctga cgcatcttgt tcacctcatg gtaaagcacc
     2761 tcttcgggta cttccatcac gacgctgcat tccttgatgt atattgtcct ggttatggat
     2821 tcgggaataa cggctattga cctgactata tcgcggatca gattagcctt ttttacgggg
     2881 tcacccgcag catcttcaat aaggaggttt attttgaaac gtatgaaatc tgtctcattt
     2941 tcattgagat aattccggaa ttcttcgttg cttactttcc ttgaccagga atcagggtct
     3001 tcattttccg gaagaaggac aatccttaca ttcattcctt cctcgagcac caggtcggtt
     3061 ccccgcaatg aggcttttat acctgcggga tcgccgtcat acagcattgt tatgtttgac
     3121 gtaaagcgtt ttatgagcct gacctgttcg cgggtgagtg aagtgcccga agaagcgaca
     3181 acattctcca cgtttgcctc atgcagtgac atcacatcgg tgtatccttc caccaggaag
     3241 cacttgtctt cttttgttat tgcccttcgt gcctgataga taccgtaaag gatacggctt
     3301 ttgtgatata tctccgactc cggggagttg agatattttg ctgcctttgc atccgtcttc
     3361 aggattcttc ccccgaaacc aagcacctga ccggaaaggg aatggatggg aaatattatt
     3421 cttccgctga atctgtcgaa gatacggtct tcgtgttcaa ttgacaggcc tgtttttaca
     3481 aggaaatcct ttctgtgacc gtcatccaga gctttctgcg aaaaggcatc gcgtttctcc
     3541 aggctgtatc ccgtttcaaa tttcttgagg gtgttatgcc tgaatcctct ttccctgaaa
     3601 tatgaaagtc ccaccgaccg gccctcgtcg ctgttgaaga ggttttccga gaactgccgg
     3661 gatgcgtatg cagtaacgac aagaaggctt tccctttcgt tctgcttttc aatttcctcc
     3721 tgagaaagct ctctttcaac tacttcgata tgatatttcc ttgccaggaa tttcagggcc
     3781 tccggatagg agaggtgttc gtgttccatt atgaaattga ccacgtttcc caccttgccg
     3841 catccgaagc atttgaatat ctccctggaa ggcgacacgg tgaaagaggg tgttttttca
     3901 ttatgaaagg ggcagaggcc gagataattt gcaccccgtc ttttgagggg gacaaattcc
     3961 tgtaccacct caactatctg ggcggcattg agtatgcgtt ctatggtgga gcggtcaatc
     4021 atcctccaaa gatatcagat aattccgtac tgttcgagaa gattccgggt cagtatcctt
     4081 accccgtccg atgctttttc ctttatcacg ttgatattct taactgaagg aacatataca
     4141 tcatgcggat caatgagcca gagggaggcg ttccgcggag caaagccgac aagtcccgca
     4201 gcaggataaa cattgagtga ggaaccgatc accgcaaaaa tatctgccgt ggaagttatt
     4261 tccactgctt catccatcag cgggactgcc tcgccaaacc agacaatatg cggccggagc
     4321 tgacttccct tctcgcagag atcccctgga ttgacttcct tgtatcctat gtcgtaaacc
     4381 agtgaaggat ctttggtact cctcactttc gtcagttccc cgtgcagatg gaggatcttc
     4441 gtgcttcccg ccctctcatg aagatcgtcg acattctggg tgatgacgtg aacgtcgaaa
     4501 tacttttcaa gtgcggcaag gcccttgtgt ccgtcatttg gttcacatac cgcaagctgt
     4561 gtccttcttt cattatagaa cctcagtacc agatccctgt tttttgccca tcccatgggt
     4621 gaagcgactt cattcacatc atattcctcc catagtccgc ccgtctccct gaaggtccgt
     4681 ataccgcttt ccgccgacat tccggcacca gtgagtatta ccagtttttt catcataatt
     4741 tgtattgttg taaaaatacc aaataataag tcattcttca tcgtttatta ttttgtactc
     4801 acggaacgat gcatttattt tgagaagtaa ggtgaatcag tggaaccccg gcccgacatt
     4861 aaaacaattt ggacagggac ggtaaacagg cagaatccct gaaggtgaac gaccccgtta
     4921 accgttcagg aagtcagaat aaacagaaac ccgatgttac acagataccg gataaaaaat
     4981 aaatatggga taagatccct tgcattcagc atatttcttt ttcttgcgcc tgtcctggct
     5041 gccgctgccc gggatcccga ttccctgaaa tttaaatctc tcgatccgca ttattttcat
     5101 ctgcaatatc ttaaagagaa tcctgcctgg cttatagatg taagaatgtc ttttgagtac
     5161 aggggcagaa ggatcaggga tgcaataaat attccctcgg cgaaggaatt gtatgctttt
     5221 acagacacga tcagcaaaaa cacggcttta ttcctgtact gtactgatgg cttcagaagc
     5281 aaacaggctg ccaggttgct gtatgacaag ggattccgga atctcttcaa tcttgaaggg
     5341 ggtatcatag cctggaaaaa ggatggtttc cctcttgaga aggggagggt taagattaag
     5401 aaaagaaaac gctgagacaa tttatcgctc tccgtttgag acataaaagc acagaacaat
     5461 ttcattactt ttgcaggcgg atcaataaca taaaaacatg ttttcgggaa tagttgaaga
     5521 agcggcaccg gttgtaaaac tcgagaggga gcaggacaat cttcatatta taatgaaatg
     5581 ctcattcacg catgagctca ggatagatca gagcatatcg cataacgggg tatgccttac
     5641 tgtcgtaaaa acggaaggcg actgctatac agtcacggcc ataaaggaaa ctcttgaaaa
     5701 gtcaaatctc ggtcttctga aaaccggcga caaggtaaac cttgaaagaa gcaccaggct
     5761 ggatgcccgg cttgacggac acatggttca ggggcatgtt gaccagacgg cagtctgtac
     5821 ggaagttacg gaagcaggcg gaagctggta ttataccttc gaatatgaac ctctgcagga
     5881 tgattacctg acagttgaaa aagggtcggt tgccgtgaac ggagtcagtc tcaccgtagt
     5941 caactcacag ccgaaatcct ttcaggtcgc aatcattccc tttacatggg aagtgaccaa
     6001 tttccaccag atcaaggcgg gaacggtggt gaacatcgaa ttcgacatcc tggggaaata
     6061 tattgcaaag atagtcaggc agcagcttca gaaataaacc tgatatggct gccggagaaa
     6121 taaccgcaac aggtcttgcg atgcagatat cagtctcaga tcagaaatga aactcagatg
     6181 actgaccgga tccggatgac aggtcccata tcagtttttt cccgaatccg agccggttgt
     6241 cggtatattt cagatagctg atctttacga cccacagcct tgtgtccata agcatggcaa
     6301 cgggaaatct tttcagatag gcactttctg ccctggcagc catttctccc tccggttcgt
     6361 aaacagttcc ggagaactga attccgcgta tcctgccgat aattccggtt tcaagcgcaa
     6421 cggttccggc taccgaggca ttcttcagga attcctgccc gtgccttgtc ttcagatcgg
     6481 tggtaaagac cagggaattt tcctcttcca ggtaaacata gaagcaactg gcacaccatg
     6541 gtgtttcacg gaccgaggtt gctattgtca gcacatggtg ctttctgaaa aaccttttaa
     6601 tcctcctgtc aaccattctt catccatctg tgtaaaggtg catcaaaacc ggcaagcgac
     6661 acaatccttt ccaccactgt cataacaaga tcatcaatat ttcccggcct gctgtagaat
     6721 gaaggcgatg ccgggcacac tattcccccg gcaagaagga tggtttccat gttcctgaca
     6781 tggacaaggt tgtagggtgt ttcgcgggga accactatca gctttctttt ttccttcagc
     6841 attacatctg cagcccttgc aatgaggtca tccgagctgc cgctggctat ccttccgaga
     6901 atacccattg aggccggaca gattatcatg gtgtcgtagt cggatgaacc ggaagcaaag
     6961 ggtgcatgga aatccctgct gtcatattcc ttttcgggag gaaacgcctc gtaatccctg
     7021 ccattctcat atttccatat ctcctttccg ttatcagaaa atataacggc ggtttcagcg
     7081 ggagggtcct ttatcttcct gaggaaatcc agcagtttca gcgcatagac cgatccgctg
     7141 gcacctgtaa cggcaaggat tattttgcgt tcagactttt ccatagtcag aaattgaatg
     7201 gctgataaaa gtagtaatta aagttattcc caacagcagt gacattttta ttatctttgt
     7261 tcgttttttc agttcataaa taaaaagtgt aagatgaggg gaaaatttcc tgaatataag
     7321 actcttgacc tgtcccgtgt gaacaaggac atactgaaga tatgggacag ggacaatatt
     7381 tttttcaaga gtataaggca gcttaacggc aagggggaat acattttcta tgaaggacct
     7441 ccctcggcaa acggtatgcc gggaatccat cacgtcctgt cgagaaccat caaggatgcc
     7501 atctgcaggt acaggacgca gtgcggttat gaggttaagc gcaaggccgg atgggatacc
     7561 cacggcctgc ccattgagct gggagttgaa caggctctcg ggataacaaa ggaagatatc
     7621 ggaactgaaa agatctcaat agaggaattc aacagggcct gccgcatcga tgtgatgaaa
     7681 tacaccgatc agtgggaaga ccttacgcac aggatgggct actgggtcaa catggacgat
     7741 ccgtacataa cctacgacaa ccgttatatc gaaacgctgt ggtggctcct gaaacagctt
     7801 tattcaaagg gttacctgta caaggactat accatacagc cgtactcccc ggcagcgggc
     7861 accggactga gttcacatga actgaacctg ccgaactgct acagggatgt aagggacaca
     7921 acctgtatag ccctgttcag gcttataagg aacgaaaagt cggaattcct ttttgaagac
     7981 attgataccg cagacgtgca tatgatggcc tggaccacta ccccctggac attgccgtcg
     8041 aacactgccc ttgcagtggg aaaggatatt gaatacataa aggttcgcag cttcaatccc
     8101 tattcgggag aacccattac tgtcattttt gccggagatc tcagggatgc catgttcccc
     8161 ggcacggatg agagtgccga tctgccggaa tacagggcgg gtgacaggaa agtacctttc
     8221 agggtacttg accggtttcc gggaagcaga cttgaaggta ttgattacga acagctcatc
     8281 gactgggtga aacccggtga aggagctttc aaagtgctga cgggagattt tgtgactaca
     8341 gaggacggta cgggaattgt gcatattgcc ccgaccttcg gagccgatga ctacaaaata
     8401 gcccgggaat acggtgttcc ggccatgctt ctcaggatga aggacggcac ttttggcccc
     8461 atggttgaca aaaagggata tatggtgccc gtcagcgacc tggatgagaa atttgtcagg
     8521 gaacgtgtca gtctgaacag ctatggtcct tttgaaggcc ggcccgtgaa gaacgaatat
     8581 gatgaaaata ttgatccgga tacggatacc ctggatgttg acatcgccgt catgctcaag
     8641 cagaccggca aggtgttcag gattgaaaag atggaacaca gctatcctca ttgctggcgt
     8701 accgacaagc ccgtactcta ttatcccatt gatgcatggt tcatacgtac cacggcattc
     8761 cgcgacaggc ttatagaact gaacaatacc atcaactgga agcctgcttc aacaggatcg
     8821 ggaaggttcg ggaaatggct ggagaacctc gtggactgga acctttcgcg ttcacgtttc
     8881 tggggaactc cccttccggt atggaggacc gatgacggga aggaatcagt atgcatcggt
     8941 tcggtgagcg agcttaaagc cgaaatggaa aagtcggtca tggcgggttt catggagagc
     9001 aatcctcttg ccacattcac agaaggcgac aattcgccgg agaactacaa caaactggat
     9061 cttcaccggc cttttgtgga caatatcata ctggtgagtc cgtcgggaag gaagatgttc
     9121 cgtgaaccgg accttatcga cgtatggttc gattcgggag ccatgcccta tgcacagtcc
     9181 cattaccctt tcgagaacag ggataatttc agtgaaatgt tccctgccga tttcgttgcg
     9241 gaaggc
//
LOCUS       DAOSPX010000099         9078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7300_length_9078_cov_4.687465, whole genome shotgun sequence.
ACCESSION   DAOSPX010000099 DAOSPX010000000
VERSION     DAOSPX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7300_length_9078_cov_4.687465"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PLZ75_06455"
     CDS             <1..1326
                     /locus_tag="PLZ75_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOS72076.1"
                     /translation="LNVKSTADNPGKGSIILSLEGTSAAAANSEGYELHITAKQLRLT
                     AATHAGLFRGVQTIRQLLPARIEMTERQQGPWKIATGTISDYPEYSHRGAMLDVARHF
                     FGIDDVRRFIDFLAYYKMNSLHLHLSDDQGWRIEIKSWPNLTLHGSTTEVGGGSGGFF
                     TQEQYRAIVEYAGENYITVIPEIDMPGHTNAALASYPELNCDGKATELYTGIEVGFSS
                     FCIHKEITYKFIDDVIREISEMTPGPYFHIGGDESHATKLSDYIPFVERVQEIATSHG
                     KQCIGWDEIANAKLKPGSIAQNWANPKNAGKAVAQGAKILMSPANKAYIDMKYDKTTK
                     YGLQWAGFVEVDVSYNWDPATLVPGVGREDIVGVEAPLWTENITNIKEIEYMVFPRLA
                     GIAEIGWTPAESRKWEEYRTRLGRHGERFDAMDINYYKSPLVDWTGLDE"
     gene            complement(1330..2697)
                     /locus_tag="PLZ75_06460"
     CDS             complement(1330..2697)
                     /locus_tag="PLZ75_06460"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOS72077.1"
                     /translation="MKAYRYLLPLLIIVFCTACRSGGPETKEKESAYTDTPVLEHIFP
                     FQTQHCHGSSVAELPNGDLMVAWFHGSGERTADDVEIRGARRSHKTGRWSKPFILADV
                     PDFPDINPVIFIDGKSRLWLVWYTVLAYRWESSLLKYRISENYMQESGPPEWKWQDMI
                     HMKPDGSSPDGIGRNDRFVQILERKYDELYRDLVSKGFINNETGPVSDEMWKKALDGY
                     LAIAEGKGFTGPGTDISEKGERIRTELGFPLQRRIGWQSRNKPLITGNRLLLPLYSDG
                     FDFSIIAITDDWGETWQFSEPLVGAGSIQPSLALCRDGSITALMRDNGPPPARLARST
                     SSDTGKSWSLVEDTDIPNPGSAADVVVLKSGNWALVHNDTEQGRHRLSVWLSEDEGKS
                     WPFRKILAEGTAGSESRSHYPAIIQGSDGMIHISYTNQIPGPEGRPSLKNIVHASLSE
                     KWLTD"
     gene            2887..3732
                     /locus_tag="PLZ75_06465"
     CDS             2887..3732
                     /locus_tag="PLZ75_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOS72078.1"
                     /translation="MKKILLMVFLSVIAINCISQDDKLNIIVIGAHPDDCDEFAGGTT
                     ILYSKLGHRVKFVSMTNGDAGHQSMGGGALAKRRMLEAREAGKRFGVEYLVIDNHDGE
                     LMPTLENRLKIIREIRNWKADIVITHPPDDYHPDHRNTSILVQDASYMVIVPNVAPDT
                     PPLKKNPVFLYPGFSSPEIAVDIDAVFDQKVYAVSAHESQFFEWLPWTEGTLDKVPSG
                     REERLKYVAEWFGTVSETEKACLKKWYGEARAVKVLHAETFRYAPYGRKPSEEETRKL
                     FPMIK"
     gene            3759..5714
                     /locus_tag="PLZ75_06470"
     CDS             3759..5714
                     /locus_tag="PLZ75_06470"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOS72079.1"
                     /translation="MKKSILFFLSVFFCCNSSAQKYELLSPDRKLKAEIEINRGISAD
                     LKRNEARIVKLSNLELETGCITQSGFRYKIKKTNRESVNEIVRPEIREKASELVNAYN
                     ELEIRFRADYSVTFRLFNEGLAYRFTTFSKDSLIIKKENLEISLGENDSARFQSSKSF
                     NSSYETPYEHKKLNDIEQGQLCNLPFLVQKQDGSFVMITEADLYNYPGLWLRGTAGEH
                     LEAANPPFPEAFSSTGNAYVQGQVSETSDYIASVSGTRTYPWRIFAIAGAEEGLVSNS
                     MVYLLATPCAIEDPSWIRPGVVMFDWWAKHNIYGVDFRSGINTETAKYFIDFCAKHGF
                     RYFLFDDGWCPRDNLLEPVPGLNMEEVTAYASSKGVDIMLWVIWRTLEKQWAEAFSLF
                     ESWGIKGIKVDFMNRDDQVMVEFYERVARMAGEKKMVVNFHGAYKPCGLRRKYPNVLT
                     REGLIEYEYNGWTDEADPEHHNLLPYIRMFAGPMDYIPGSMRNSTKDNFRAVGDYPME
                     QGTRAHSMALFVILNSPMTMLPDSPSDYYREKECAGFLTKIPVEWDETRLLKGKIAEY
                     TVIARRSGDNWYIGAITNWNSREIDLDTKFLNPGKYRIEAITDGINSDKRAEDYRKIQ
                     MECEAGDILKLRLASGGGWVAEITPVR"
     gene            5916..7217
                     /locus_tag="PLZ75_06475"
     CDS             5916..7217
                     /locus_tag="PLZ75_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72080.1"
                     /translation="MEKSFYLAMLFALTVSCTSPGVKKNRMLAGQIRNNEYYAFTEQK
                     ALEMIGSGFNAGDGYLEVWIRDYNTFIELAAAVHPAEELRENLMVFFRMQAEDGGILD
                     GFTPVAAAGEVGYDFYYSDLEPRYAGHKNTVETDQESSLVQAVCKYIKLTSDSAVLSC
                     EIAGKSIAERMEMAMQFLMDHRFSEEYGLIYGATTADWGDVQPGHGWGVAWDENTHPA
                     IDIYDNAMFIIALENLTEAIPATREKWVPVREMIAQNARKHLWDEGNRKFIPHIYLEK
                     GSPFPPDFDENQIYYFGGTAVAIEAGLLSKDEIKASLDEMVRRVKLAGAASIGLTLYP
                     PYPEGFFENKIMSVEYSYQNGGDWTWFGGRMIQQLISHGFVEEACEQILPMVKRVKDN
                     NGFYEWYTVDNKPRGSGTFRGEAGVLFTAIRMLRDYAAGID"
     gene            7248..8069
                     /locus_tag="PLZ75_06480"
     CDS             7248..8069
                     /locus_tag="PLZ75_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOS72081.1"
                     /translation="MIFNETYSLLRDRHAREIENLVLSDVRIGLYLTAVRLSDNSVGV
                     SSTLPAEHPFCLKENRNFDDFSPLKIRGKRVTEILETDSKSRIVSGLKTAVLNAVSSG
                     IISSGKYRIMENCDPVQLLDLEKKQTITIVGAFHSYIRKISETGNRLFVLEKNKNALH
                     PEFRNYYIEAAHYEKVVPVSDVLLITGQTLVNDTIDELLALARPGCRIIVAGPSGGII
                     PDVLFEKGVAIVGGMRITRPEIVFSIIGEGGLGYHLFKYCAVKICILREDEGRAE"
     gene            8050..>9078
                     /locus_tag="PLZ75_06485"
     CDS             8050..>9078
                     /locus_tag="PLZ75_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72082.1"
                     /translation="MKAGLSETWIKASIAGTMWAASEIVLGSFLHNLRVPFSGNILTA
                     IGIVILVSVGHIWNDRGIFWRAGLICALMKTMSPSAIIFGPMIAIFSESLLLELPVRI
                     FGRSIAAYLLGAILAMSWNLFQKIANYIIIYGSNIIEVYNNLLRWAQKQLEIHTDIVW
                     LPIIFLLVIFAVFGLIAGMAGIMVGRKMLRHPDPEFSSPVNGTDAGISRKPETEFSYS
                     LAWLFFDILLMIFAFLIIYHTPWYVWTLVISGMVLVWSVRYKRAMRQLSKPKFWLFFV
                     FVTLLTAFAFTVAKGGDISWQQGLLTGIQMNFRAVVIVVGFASLGTELYNPAIREFFQ
                     KTAFKNVPL"
BASE COUNT         2582 a         1905 c         2233 g         2358 t
ORIGIN      
        1 ctgaatgtta aatcaacagc tgataatccc gggaagggtt ccataatact gagtctggaa
       61 ggcacttcag ctgccgcagc gaacagtgag ggctatgagc ttcacattac agcaaaacag
      121 ctgaggctta ctgcagccac acatgccggc ctgttcaggg gcgttcagac catcaggcaa
      181 ctgctgcccg caaggattga aatgactgag cgacagcagg gaccatggaa aattgcaacg
      241 ggaacaatca gtgattatcc ggaatattcc cacaggggag caatgcttga tgttgcccgc
      301 cattttttcg gcattgatga tgtaagacgc tttatcgatt tccttgcata ttacaaaatg
      361 aacagtctgc acctgcatct gtcggatgac cagggatgga ggatcgaaat caaatcatgg
      421 cctaatctca ccctgcatgg cagtacaaca gaggtcggcg ggggatccgg cggatttttc
      481 acccaggaac aatacagggc tattgttgaa tatgccggag aaaactatat aaccgtaatt
      541 cctgagatag atatgcccgg acataccaat gctgccctgg catcctatcc cgaacttaac
      601 tgcgacggaa aggccacaga gctttatacc ggaatagaag tcggtttcag ttcattctgc
      661 attcacaagg aaataaccta taagtttatt gacgacgtga tcagggaaat ttcggaaatg
      721 acaccgggtc cgtatttcca tatcggagga gatgaatcgc atgcaacaaa actatcggat
      781 tatatacctt ttgtggagag ggtccaggaa attgcaacaa gtcacgggaa acagtgtata
      841 ggctgggatg aaattgcaaa tgcaaaactg aagccgggca gtattgccca gaactgggca
      901 aatccgaaaa atgccggaaa agcggttgca cagggggcaa aaatccttat gtcgccggca
      961 aacaaggcct atattgatat gaaatatgac aaaacaacaa aatacgggct gcagtgggca
     1021 ggatttgttg aagttgatgt ttcctacaac tgggatcccg ccacgcttgt tcccggtgtt
     1081 ggcagggagg atatagtggg ggtggaggca ccgctgtgga ccgaaaacat aacaaatatc
     1141 aaagaaattg aatatatggt tttcccgcgt cttgccggaa tagccgagat aggatggact
     1201 cccgctgaat caaggaaatg ggaggaatac aggacaaggc tgggcagaca cggggaacgg
     1261 tttgatgcaa tggatatcaa ttattacaaa tcaccccttg ttgactggac aggtcttgat
     1321 gagtaaaaac taatctgtca gccatttctc tgacagactt gcatgaacta tatttttcag
     1381 ggaaggccgg ccctcgggtc ccggaatctg gttggtatat gatatgtgaa tcataccgtc
     1441 gctgccctgt atgatggcgg ggtaatgact tcttgattcg cttccggctg ttccctcggc
     1501 aagaatcttc ctgaaaggcc agcttttccc ctcatcttct gaaagccaga ctgacagccg
     1561 gtgtcttccc tgttcggtat cattgtgtac cagggcccag ttcccgcttt tcagcaccac
     1621 tacatccgcg gcacttcctg gattgggaat atccgtgtct tcaaccaggc tccagctctt
     1681 tcccgtatcg gaggatgtgc ttctggcaag tctggcaggg ggaggaccgt tatccctcat
     1741 cagtgccgtt atggagccat ccctgcacag tgccagggaa ggctgtattg atcctgcacc
     1801 aaccaggggc tcgctgaact gccatgtctc accccagtca tcagttatgg caatgattga
     1861 aaaatcaaat ccgtcggaat ataagggcag caataaacgg tttcccgtaa taagaggctt
     1921 gttccggctc tgccatccta tcctgcgctg aaggggaaaa ccaagctctg tcctgatcct
     1981 ttctcccttc tcactgatat cggttcccgg tcccgtgaat cccttcccct ctgcaatagc
     2041 gagatagccg tcaagggctt ttttccacat ctcatcactt acaggccctg tttcattgtt
     2101 tataaaaccc tttgagacaa gatccctgta cagttcatca tatttccttt caagtatctg
     2161 tacgaagcgg tcattccttc ctattccgtc aggtgaactg ccatcaggtt tcatatgtat
     2221 catatcctgc catttccatt cgggcggacc ggattcctgc atgtaatttt cacttatgcg
     2281 gtatttcagt aacgatgatt cccagcggta ggcgagaaca gtgtaccata caagccacaa
     2341 ccgggattta ccgtcaatga agattacggg attgatatca ggaaaatccg gtacatcagc
     2401 cagaatgaaa ggcttgctcc atctgcccgt tttatgactg cggcgggctc cccgtatttc
     2461 cacatcatca gcggtccgct caccgcttcc gtgaaaccag gcaaccatca gatccccgtt
     2521 gggcagttcg gcaacggatg aaccatgaca atgctgcgtc tgaaaaggga aaatgtgttc
     2581 cagaacagga gtatccgtgt aagcagattc tttttctttt gtttccgggc cgccggacct
     2641 gcaggcggta cagaaaacaa tgattaataa gggcagtaag tatctgtatg ctttcatgac
     2701 aggggttttc aataaaggta gctcaaaaag attaaaaatg ccatattttt tccgtccgga
     2761 atctgaacgg cccttttgct catggccccg gctttcaccg gatattaaca agcattaaaa
     2821 ccattgtcag ttgaaaaatt cctaaattta tacctgaagt tgaattaatt gctctgaatc
     2881 atgcctatga aaaaaattct cttaatggtg ttcctgtcag ttattgcgat aaactgcata
     2941 tcacaggatg ataagctgaa cataattgtt atcggtgctc atcccgatga ctgtgatgaa
     3001 tttgccggtg gtaccacaat tttatattcc aaacttggtc acagggtaaa atttgtttca
     3061 atgactaatg gagacgccgg tcatcagtca atggggggcg gggcacttgc aaaaagacgt
     3121 atgctcgaag cccgggaagc cggaaaaagg tttggagtgg aatatcttgt tatagataat
     3181 catgacggag aactgatgcc aaccctcgaa aaccggctga aaataatcag ggaaatacgt
     3241 aactggaaag ctgatattgt tattactcat ccgcctgatg actatcatcc tgaccaccgg
     3301 aatacaagta ttctcgttca ggatgcttca tatatggtga ttgtaccaaa tgtcgctccc
     3361 gatacaccac cattgaaaaa aaatcccgta tttctttatc cgggtttttc atcccctgaa
     3421 attgctgttg atattgatgc tgtattcgat cagaaagtat atgcagtctc ggcacatgaa
     3481 tcacaattct ttgagtggtt gccctggact gaaggaacat tggacaaggt accttcgggc
     3541 agggaagaga gattaaaata tgttgctgaa tggtttggta ctgtgagtga aacagaaaaa
     3601 gcctgtctga agaaatggta tggtgaagca agagctgtaa aagtacttca tgccgagact
     3661 ttcagatatg ctccctatgg acgaaaaccc tcagaagagg aaaccagaaa actatttcca
     3721 atgattaaat aaataaaacc attcaaaatt tcgaaatagt gaaaaagtca atacttttct
     3781 ttttatccgt ttttttctgc tgtaattcca gcgcccagaa atatgagctt ttatctcctg
     3841 acaggaagct taaggcggaa attgaaataa accgggggat ctcagcggat cttaagagga
     3901 atgaggcccg gattgttaaa ctgagcaatc ttgaactgga aaccggctgt ataactcagt
     3961 ccggattcag gtataaaata aaaaaaacaa accgggaatc agtcaatgag attgtcagac
     4021 ccgagatccg tgaaaaagct tcggaacttg taaacgcata caatgaactt gaaataaggt
     4081 tcagggcaga ttacagtgta actttcagat tattcaacga aggacttgca tatagattta
     4141 caaccttttc gaaagacagt ctgataataa aaaaagaaaa tctggaaatt tccctgggag
     4201 aaaatgattc cgcgagattt cagtcatcaa agagcttcaa ttcgtcatac gaaactcctt
     4261 atgaacacaa aaagcttaat gatattgaac agggacaact atgcaatctc cctttccttg
     4321 ttcaaaaaca ggacgggtca tttgtcatga tcaccgaagc agatctttac aattatccgg
     4381 gattatggct caggggaaca gccggggaac atctggaagc cgctaatcct ccgtttcccg
     4441 aagctttttc ttccacgggg aatgcttacg tgcagggtca ggtatcggaa acttcggact
     4501 atatagccag tgtcagcggt acacgtacct atccgtggcg gatttttgca attgccggtg
     4561 cggaggaggg tcttgtttca aacagtatgg tttaccttct tgctacaccc tgtgcaatag
     4621 aggatccttc ctggatcagg ccgggagtcg tgatgttcga ctggtgggcc aaacataaca
     4681 tatatggtgt tgatttcagg tcagggataa atacagaaac agcaaaatat tttattgatt
     4741 tctgtgcaaa acatggtttc agatatttcc tttttgatga cggatggtgt ccgcgtgaca
     4801 atcttcttga accggttccg ggtctgaata tggaagaggt aaccgcttat gcttcatcaa
     4861 aaggcgtgga tataatgtta tgggtaatct ggcggacact cgagaaacaa tgggcggaag
     4921 ctttcagcct gtttgagagc tggggaataa agggaattaa agtggatttt atgaaccgcg
     4981 atgatcaggt aatggttgag ttttatgaga gagttgcccg tatggcaggg gagaaaaaaa
     5041 tggtggttaa tttccatggt gcatacaaac cctgcggact gcgcaggaag tatccgaatg
     5101 tacttacccg cgagggactg atagaatatg aatataacgg gtggacagat gaagctgatc
     5161 cggaacatca taatctcctg ccatatatcc ggatgtttgc cgggccaatg gattatatcc
     5221 ccggatccat gcgtaattca acaaaggata atttcagggc tgttggcgat tatccgatgg
     5281 agcaggggac acgcgcccat tcaatggccc tgttcgtgat ccttaacagc ccgatgacaa
     5341 tgcttccgga ttcaccttca gattattacc gtgaaaagga atgtgccggg ttcctgacaa
     5401 aaatcccggt tgaatgggat gaaacacggt tgctgaaggg aaaaattgcc gaatatactg
     5461 ttatcgcacg gagatcaggc gataattggt atattggagc aataacaaac tggaacagcc
     5521 gtgaaattga cctggataca aaattcctga atcccggaaa atacaggatt gaagcaatta
     5581 ccgacggcat caattccgat aaacgggcag aagattacag gaaaattcag atggaatgtg
     5641 aggcaggtga tatattgaaa ctcaggcttg cttccggagg tggctgggtg gcagagataa
     5701 ccccggtcag gtaaaatctc attttgattt ttacaggtat cgcgggaaag gatccgggcg
     5761 aagatgtgta tttatttttt acaaatgata tattcgcaat aaataaatac aaggtaattt
     5821 ctgaagcagt tccgggctgc aaacagggat ttgctgaaaa gaacaaattt ttcttaggaa
     5881 gaatacaagt aataattttt taactgacag actgtatgga aaaaagtttc tacctggcaa
     5941 tgttatttgc cttaaccgtt tcatgtactt cacctggcgt taagaagaac agaatgcttg
     6001 ccggacagat ccggaacaat gaatattatg cctttactga acagaaagcc ctggaaatga
     6061 tcgggagcgg attcaatgcg ggcgacggct acctggaagt ctggatcagg gattacaata
     6121 ccttcattga acttgccgcg gcggtgcatc cggctgaaga actccgggag aatcttatgg
     6181 tgtttttcag gatgcaggcg gaagacggag gcatactcga cggattcacc cctgtggccg
     6241 ctgcgggtga ggtgggatat gatttttact attcggatct tgaaccccgt tatgcaggac
     6301 acaagaatac cgtggagaca gaccaggaat cctcactggt gcaggctgta tgcaaatata
     6361 ttaagctgac ttccgacagt gctgttttat cctgcgaaat tgcgggaaaa agcatagctg
     6421 aaagaatgga aatggcaatg cagttcctga tggatcatcg tttcagtgag gaatacgggc
     6481 tcatatacgg agccacaacg gcagactggg gtgatgtaca gccgggacac ggatggggtg
     6541 ttgcatggga tgagaacact catccggcaa ttgatattta tgataatgca atgtttatta
     6601 ttgccctgga gaatcttact gaagcaattc ctgcgacaag ggaaaaatgg gttcctgtca
     6661 gggaaatgat tgcacaaaac gccagaaagc atctctggga tgaggggaac aggaaattca
     6721 ttccgcatat ttatctggaa aagggatcac cgtttcctcc ggattttgat gaaaatcaga
     6781 tttattactt cgggggtacg gcagttgcaa ttgaggcagg cctgctttca aaggatgaaa
     6841 taaaagcttc cctggatgaa atggtgagaa gggtaaagct tgcaggagct gcatcaatag
     6901 gactgaccct gtatcctccc tatccggaag gattctttga gaataaaata atgagtgttg
     6961 aatacagtta tcagaacgga ggagactgga cctggttcgg gggaaggatg atacagcagc
     7021 tcatcagtca cggcttcgtt gaggaagcct gtgaacagat actgccgatg gtaaaaaggg
     7081 taaaggacaa taacggattc tatgaatggt ataccgttga taataaacca aggggatcag
     7141 gcacattcag gggagaagca ggagtgcttt tcaccgcaat acggatgttg cgcgactatg
     7201 ctgccgggat tgactgattc catgccctga attgataaag agaaggaatg attttcaatg
     7261 aaacttacag cctgctgagg gacagacatg ccagggagat tgaaaacctg gtgctatcag
     7321 atgtcagaat aggtttatat ctgacagcag tcagactgtc ggataattct gtcggggtct
     7381 cctcgacact gcctgctgaa caccctttct gccttaagga aaacaggaat tttgacgatt
     7441 tctcaccgct taaaatcagg ggaaaaaggg taactgagat actggaaaca gacagtaaat
     7501 cccgtattgt ttcaggtttg aaaacagctg tcctgaatgc agtgtcatcc ggaattattt
     7561 catcggggaa atacaggata atggaaaact gtgatccggt tcagctgctg gatcttgaga
     7621 aaaaacagac aatcaccatc gttggggcat ttcactccta tatcagaaaa atatccgaaa
     7681 cagggaacag gctgtttgtt cttgaaaaga acaaaaacgc ccttcacccg gaattccgga
     7741 attattacat tgaagctgcc cattatgaga aggtggttcc tgtatccgat gtgctgctga
     7801 ttaccggtca gacactggta aatgatacca tagatgagtt gctggcactt gcccggcccg
     7861 gatgccggat aattgttgcc ggcccctcag gcggcataat acccgatgtg ctgtttgaaa
     7921 agggtgtagc cattgtggga ggcatgcgga tcacaagacc ggaaatagta ttcagtatca
     7981 taggtgaagg aggcctcgga tatcacttgt ttaagtattg tgccgtaaag atttgcatac
     8041 tgagggaaga tgaaggcagg gctgagtgaa acatggataa aggcatcaat tgccggtact
     8101 atgtgggccg cttccgaaat agtgctgggg agcttcctgc ataacctgag agtccctttc
     8161 agcggcaata tcctgacggc aatcggtatt gtcattctgg tatcggtagg acatatctgg
     8221 aatgaccggg ggatattctg gcgggcagga cttatttgtg cattgatgaa aaccatgtcg
     8281 ccaagtgcga taatattcgg accaatgatt gcgatatttt ccgaatcact cctgcttgaa
     8341 ctccccgtaa gaatttttgg aagaagtatt gcagcctatc ttttaggggc catactggca
     8401 atgtcatgga atctttttca gaaaatagcc aattacatca taatttacgg atcaaatatc
     8461 atagaggttt acaataatct gctgagatgg gcccagaagc agctcgagat tcataccgac
     8521 attgtatggc ttcccataat cttccttctt gtgatctttg ctgttttcgg actgatagcc
     8581 gggatggccg gcatcatggt cggcaggaag atgctcaggc atccggaccc tgaattcagc
     8641 agtcccgtga atggtacgga tgccggtatc agccggaaac cggaaacgga attcagctat
     8701 tcccttgcct ggctcttttt tgatatcctt cttatgatct tcgcctttct tattatctat
     8761 cacactccct ggtatgtctg gaccctggtg ataagcggga tggtccttgt ctggtcagta
     8821 cgctataaaa gagccatgcg tcagctgtca aaacctaaat tctggctgtt ttttgtcttt
     8881 gtaacactgc tgaccgcatt tgcctttacc gttgcaaagg gaggtgatat ttcatggcag
     8941 cagggtctcc ttacaggtat ccagatgaat ttcagggccg ttgtaatagt tgtcggattt
     9001 gcaagcctgg gtacggaact gtataatccc gccataagag aattctttca gaaaaccgcc
     9061 ttcaaaaatg ttccgctg
//
LOCUS       DAOSPX010000100         9070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7310_length_9070_cov_4.439712, whole genome shotgun sequence.
ACCESSION   DAOSPX010000100 DAOSPX010000000
VERSION     DAOSPX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7310_length_9070_cov_4.439712"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLZ75_06490"
     CDS             complement(<1..866)
                     /locus_tag="PLZ75_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS72083.1"
                     /translation="MVSGYSFLKSMITRKAAISGMPVALSVELTNHCNLKCPECLCGS
                     GRMTREKGFMDISLFTRIIDELKPFLLEVSLYFQGESMLHPGLDSFLEKARGIRTTLA
                     TNGHFLSPDNAEMLALSGLNKLIVSLDGMDQDSYSLYRVNGNFRTVAEGIGNMSEAVR
                     KHGSRAELVIQFLVNRENEHQIPDVRRFARKMRATLKLKSMQVINEDSHEKWLPSDKK
                     FRRYQLKGNKYTILSNLPDSCRRLWFNPVITWDGKVIPCCFDKDAGHILGDLNENTFR
                     EIWTGRAYRLFR"
     gene            1132..2496
                     /locus_tag="PLZ75_06495"
     CDS             1132..2496
                     /locus_tag="PLZ75_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS72084.1"
                     /translation="MKIRMNIKSAAIFLLLALAFGSLSAAEAGNKGRAADAGKTGRAA
                     EAARSGKAAEQELIVLRGRVIDAETKSPLVFASVAVTESNVGIITNIDGEFTLKISPD
                     LVSHNIEVTFLGYKNKIVPVSSLRDDGFKNTIALETAPIPIKEVIVRPLDPEAIVERA
                     ISRLGKNYESVPNLMTAFYRETIRKNRTYVSIGEAVMEIFKAPYNNDLRFDGARIYKG
                     RKSTDVEKMDTVLFKLQGGTTSVLQLDIAKNTEAILTTEAMKYYDYSITGVIEVDDRP
                     HYVIGFIQKPWVDQPLFMGNLFIDMETNAITEAEFGFNLSDIAAATAIFIRKKPFGME
                     VTPELATYRTKYREQDGKWYFSYSRAEVRFKVRWKRKLFSTFYTTMSEIAVTDRTDQE
                     VIKFAGKEKVRYTDIFSEKVSAFTDPDFWGDYNVIEPDQSIEAAIRKLSRRLKFSDRQ
                     DLEE"
     gene            2504..3040
                     /locus_tag="PLZ75_06500"
     CDS             2504..3040
                     /locus_tag="PLZ75_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOS72085.1"
                     /translation="MISDNEIIRRIRKGDVAQFESLFRSFYVSLVRYALSFVRDHDTA
                     GEIVQDVFYRIWAGRERISIKKSLSAYLFRAVHNSCLHLLEHRKVVEKHEREIIRERE
                     TNSGSTSELVQYNELLSAVADVLERLPPKYGQIFCMHRFEGLKYAEIAEKLQISVKTV
                     EAGMSKALKEFRKALKEK"
     gene            3037..3936
                     /locus_tag="PLZ75_06505"
     CDS             3037..3936
                     /locus_tag="PLZ75_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOS72086.1"
                     /translation="MTEKMNVKDYSGMEYYGPGRHEGDRIIDVDRAWNSLLARINESP
                     VSGTPAALPRFSSKLLKIAAALLVITGLGLAGLFISDRKLLSKKISVVTDINSKNLEV
                     TLPDGSIAILNRGTVLSYRSNYGKTERKLSLSGEAFFEIMADHGKAFTIDAGKAVVKA
                     VGTSFNVITSNPDSQVEVFVRTGKVLLSDNSGNNSILLDPGYIGTMDSGKPLKSLNRD
                     PNYMAWNTGILVYDGQTLDIVFRDLKRVYNMEIIADDPSILKETWTSPINNQSQDTII
                     RLICYSFNLGYTKEGNTYHLSKR"
     gene            3952..5511
                     /locus_tag="PLZ75_06510"
     CDS             3952..5511
                     /locus_tag="PLZ75_06510"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS72087.1"
                     /translation="MLTDNGFIGKRLKAVLLILALSLWCNVKAQENILDSLFTFTEGV
                     VNTGSALNIISRQSGYNFSYDSRLIDPGRETSLLFNNTRLKDILDAVLASDSLRYTIV
                     DRYIIISRQLPFPRAATDSIFPPSHTYITGTVVDSDSDEPLPFATIGLKNTGKGTVTN
                     ATGEFGLNIPRDRMNDTLSVSYLGYLGNEIPIKQIQGSNFQIRMQRDFISIPEIIIRT
                     QAPQEIIYRSFAAIPSNYGNTPAGLTAFYREGVMKKQKLQNYSEAVIKIFKSSYSGSF
                     FGDQIKILKSRKIDNVNMRDTLSVRLKAGLSTCLELDGVRNHFDFISRASMPEYRYSI
                     SDIVSYEDESAYVIDFEQREYAEQALYRGSLYINALDYAILHAGFEVNPKYLGRMKSS
                     FISSTSRGYDTWPQSVKYTVSYRRYSNRYFLNHVRGDLVFISKQKRKLFNTQFRVFFE
                     LAVTGIETEGVTRFDREELAPIHSVFSRTIKSYDSKFWENQDFLKPEENLLQALKNMN
                     VRLGEFTGDDQ"
     gene            complement(5505..6515)
                     /locus_tag="PLZ75_06515"
     CDS             complement(5505..6515)
                     /locus_tag="PLZ75_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOS72088.1"
                     /translation="MSGKMIKPLLWIFLIASFSCVPAKKYRDLQVTSMQNMNERDDLK
                     SSNLYLEMENRELEAKMALMDREVQKTREDISKAEKERDKALEEFRVISGRYNDLQNA
                     QEELIRGNVRETQKLLTELQAAQANLRNKEDLLRRLEETLDTRKASLDELTYELEKRN
                     ARLVELERILDAQKRIAQDLKNKVSEALLGFENNGLTVTQKNGKVYVSLEEQLLFKSA
                     SWEIDANGRNALKKLAAVLERNPEIQITIEGHTDDVPYNPSGSQLRDNWDLSVKRATT
                     VVRVLLEGSRIDPKRLTAAGHSEFMPVDARKTSDARQKNRRTDVVLTPDLTGLYNLID
                     SY"
     gene            6669..8564
                     /gene="dxs"
                     /locus_tag="PLZ75_06520"
     CDS             6669..8564
                     /gene="dxs"
                     /locus_tag="PLZ75_06520"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOS72089.1"
                     /translation="MSQGKDLLFKISSPEDLRKLNPSDLIRVSEELRQFIIDEVSNNP
                     GHFGASLGVVELTIALHYVFDTPRDKIIWDVGHQAYGHKILTGRRDVFSTNRKYRGIS
                     GFPRMSESEYDAFGTGHSSTSISAALGMAVASRQKGEKRHVVAVIGDGAMTAGEAFEG
                     LNNAGIAKSDLLVILNDNNIAIDQNVGALKEYLLDIATSGFYNKIKDKVWNLLGRAGR
                     FGPNARTLAAQLEAGFKSTLLDRSNLFEGMNFRYFGPIDGHDVMHLVKVLEDLKRIRG
                     PKLLHCLTVKGKGFRQAERHQTEFHAPGRFDKQTGAILSSDHEDSAPPYQEVFGHTIL
                     ELARMNEKIVGVTPAMPTGCSLCIMMKEMPGRAFDVGIAEQHAVTFSAGLATQGLIPF
                     CNIYSSFIQRAYDQVIHDVALQNLRVVFCIDRGGLVGSDGATHHGFFDLAFMRTIPNM
                     IVAAPMNATELRNMMYTAQSDRVSSPFSIRYPRGSGMVREWRRGFSEIPVGRARLIRE
                     GSDIAVLSIGHPGNDVDKAARKLEAESVSVCHWDMRFAAPLDEEALHSVFGRFKKIIT
                     VEDGILKGGFGSAVIEFMSDNNYSAEIRRLGIPGYFVEHGTQEELYRECGYDAVGIEN
                     TIRKMLK"
     gene            8685..>9070
                     /locus_tag="PLZ75_06525"
     CDS             8685..>9070
                     /locus_tag="PLZ75_06525"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS72090.1"
                     /translation="MKKTIILPVLVLLAASCGTRTNKADNEEWIQLFNGRDLSGWIIK
                     IKGSPAGVNYKNTFRVEDGIMKVSYDEYDRFNAEYGHIFYEQPFSRYKLRIEYRFTGN
                     QVAGGQGWAYRNSGVMLHSQSPGSMLL"
BASE COUNT         2517 a         2189 c         2195 g         2169 t
ORIGIN      
        1 tgtctgaaca gcctgtaggc ccggcccgtc cagatttccc tgaaagtatt ctcattcagg
       61 tcaccgagaa tatgtccggc atccttgtcg aaacaacaag ggatcacctt tccgtcccat
      121 gttataaccg gattgaacca caatctcctg cagctgtccg gaagattgct gagaatggtg
      181 tatttattcc ctttcagctg atagcgcctg aattttttat ctgacggaag ccatttctca
      241 tgggaatcct cgtttatcac ctgcatcgat ttcagtttca gcgttgccct catcttccgt
      301 gcaaaacgtc ttacatcagg gatctgatgc tcattttccc ggttgacaag gaactgtatt
      361 accagttcag cccgcgaacc gtgtttcctg actgcttccg acatgttgcc tattccttcg
      421 gccacagtcc tgaaattacc gttaaccctg taaagcgaat aactgtcctg gtccatgccg
      481 tcgagggaca caatcagttt gttcaggccg gaaagagcaa gcatctctgc attgtccgga
      541 gaaaggaaat ggccatttgt ggccagggtc gttcttattc cccttgcttt ttcaaggaag
      601 ctgtccagac ccggatgaag catgctttct ccctggaaat aaagactcac ctcaagaagg
      661 aagggtttaa gttcatctat gattctggtg aagagggaaa tgtccataaa gcctttttca
      721 cgtgtcattc ttccggatcc gcacaggcat tccgggcatt tcaggttgca gtgattggtg
      781 agttcaacac tcagggcaac cggcatcccg gaaattgcag ccttcctggt aatcatgctt
      841 ttcaggaatg aatatcctga taccatgaga ttcaagtatc ctgacacaga cgaagtcata
      901 ttccctcctg taaggtctgt caaggtaata aaataatttt cttaaaaaca atagggtaag
      961 accggctgtt aacggtctta gtgatggaaa gccaaagaaa agctggaaaa caccgggaga
     1021 aaatctcatg gtaaagccgg ttgttaacgg tcttagggat ggcaggtttt taaacaacaa
     1081 gcttaaagaa aaattacagt taaaatcagt tataatttaa accatagcga catgaaaatc
     1141 agaatgaata ttaagtctgc agcaatattt ctgctgttgg cccttgcctt cggttcactc
     1201 tccgctgcgg aagcaggaaa caagggcaga gctgcggatg caggaaagac cggcagggct
     1261 gcggaagcgg ccagaagcgg aaaagctgca gaacaggaac ttattgttct caggggcagg
     1321 gttattgacg cggaaacaaa atcccccctt gtttttgcca gtgttgcggt aacggaatcc
     1381 aatgtgggga taattaccaa tatcgatggt gaattcacac tgaaaatcag tccggatctg
     1441 gtcagccaca atatagaggt caccttcctg ggatataaaa acaagattgt tccggtaagc
     1501 agtctccgtg acgacggttt caagaacacc attgccctcg aaacagcacc cataccgata
     1561 aaggaggtca ttgtaaggcc gcttgatccg gaagcaatag tcgaaagggc catttcccgg
     1621 ctgggaaaga actatgaatc cgttcccaac ctcatgacgg ccttttacag ggagacaatc
     1681 cgcaagaacc gcacctacgt gtctatcggc gaggcagtca tggagatatt caaagccccc
     1741 tacaacaacg atctccgctt cgacggcgca agaatctaca agggaaggaa aagtacggat
     1801 gtcgaaaaga tggacactgt gctgttcaag ctccagggtg gcaccaccag cgttcttcag
     1861 cttgacatag ccaagaacac tgaagccata ctcaccaccg aggcaatgaa atactatgac
     1921 tattcaatca ccggtgtgat agaggtggat gacaggcctc actacgtgat aggctttata
     1981 cagaaaccct gggttgacca gcccctgttc atgggaaatc ttttcatcga catggaaaca
     2041 aatgccatca ccgaggcgga attcggattc aacctttccg acatagctgc ggcaactgca
     2101 atcttcatac ggaaaaagcc cttcggaatg gaagtgactc ccgagcttgc aacctacagg
     2161 acaaaatacc gggaacagga cgggaaatgg tatttcagct attccagggc agaggtaagg
     2221 ttcaaggtca ggtggaagag gaaactgttc agcaccttct ataccaccat gtcggaaatt
     2281 gctgtcacag accgtactga ccaggaagtt attaaatttg caggaaagga aaaagtcaga
     2341 tacactgata tcttttccga aaaggttagt gcttttaccg atcctgattt ctggggcgat
     2401 tacaatgtca ttgaacctga ccagagtatc gaagcggcaa tccgcaagct cagccgcagg
     2461 cttaaattca gcgacaggca ggatctggaa gaataattta ataatgatca gtgacaatga
     2521 aataatcaga aggatccgga aaggtgacgt cgcacagttc gagtcacttt tccgttcctt
     2581 ctatgtttca cttgtcagat atgcattatc atttgtcagg gatcatgata cggccggaga
     2641 aatcgttcag gacgtgtttt acaggatctg ggccggcagg gaaaggataa gcataaaaaa
     2701 atcactgagt gcctatctgt tcagggctgt tcacaacagc tgccttcacc tgctggaaca
     2761 cagaaaagta gttgaaaaac atgaaaggga gatcatccgc gaaagggaaa caaattcagg
     2821 aagcacatcg gaacttgtgc agtacaacga actgttgtcg gcagttgcgg atgtgctgga
     2881 acgccttcct ccaaaatacg ggcagatctt ctgcatgcac aggtttgagg gactgaagta
     2941 tgccgagatt gcagaaaagc ttcagatatc ggttaaaacg gttgaggccg gcatgagcaa
     3001 ggcactaaag gaattcagaa aagcactaaa ggaaaaatga cagaaaaaat gaatgtgaaa
     3061 gattattccg gcatggagta ctacggaccg ggaagacatg aaggtgacag gattatcgat
     3121 gtggacaggg catggaacag ccttcttgca cggataaatg aatcaccggt atccgggacc
     3181 cccgcagctc ttccccggtt cagcagtaaa ctgctgaaaa ttgctgctgc cctgctggtc
     3241 attaccggcc tgggcctggc gggacttttt atttccgaca gaaaactgct cagtaaaaag
     3301 ataagcgttg tcaccgatat caacagtaaa aacctggaag tcaccctgcc cgacggaagt
     3361 atcgcgatac tgaacagagg aaccgtcctg agctatcgct caaactacgg gaaaacagaa
     3421 agaaagctgt ccctttcagg tgaagccttt ttcgaaatca tggcagatca tggcaaagca
     3481 ttcaccatcg atgcagggaa agccgtggtg aaggctgtcg ggacttcatt caacgtaata
     3541 accagtaatc cggactcaca ggttgaagtg ttcgtcagaa cgggaaaggt tctgctctcg
     3601 gacaacagcg gaaacaattc cattctcctc gatccgggat atataggcac aatggattca
     3661 ggcaaaccgt tgaagtcgct taaccgggat ccgaattaca tggcatggaa cacgggaata
     3721 ctggtatatg acggccagac ccttgatatc gtgttccgcg acctgaaaag agtgtacaac
     3781 atggagatca tcgccgatga tccttcaatc ctgaaggaaa catggacatc acccatcaat
     3841 aaccagtcgc aggatacaat aattcgcctg atttgttata gctttaacct gggttataca
     3901 aaagagggta atacctacca tctttcaaaa agatgaccct tttcatgaaa tatgcttacg
     3961 gacaatggct tcatagggaa aagactgaag gccgtcctgc tgattcttgc actcagcctc
     4021 tggtgcaatg taaaggcaca ggagaacatc ctggattccc tctttacatt caccgaaggc
     4081 gtggtgaaca cgggcagtgc tctcaatatc atcagcaggc aaagcggcta taatttcagc
     4141 tatgacagcc gtcttattga tcccggaagg gaaaccagcc tcttattcaa caataccagg
     4201 ctgaaagaca tcctggatgc cgtcctggcc agtgattccc tcagatacac tattgtcgac
     4261 aggtatatta tcatttccag gcagctgccg tttccccggg ccgcaaccga ctcaatattt
     4321 ccccccagcc atacctatat caccggaacc gtggttgatt cagattcgga tgaaccattg
     4381 ccctttgcga ccatcggact gaagaatacc ggaaagggaa ccgtgacaaa tgccacgggg
     4441 gaattcggac tgaacattcc ccgcgaccgg atgaatgaca ccctgtcggt ttcctatctt
     4501 ggctacctgg gaaatgaaat acccataaaa cagatacagg gcagcaattt tcagatccgc
     4561 atgcagaggg attttatttc cataccggaa atcattatca ggacacaggc tccccaggaa
     4621 atcatttaca ggtcatttgc ggccatacca tcgaactacg gcaatacgcc ggcaggcctg
     4681 actgctttct accgcgaggg cgtgatgaag aaacagaaac tgcagaacta ttctgaagcc
     4741 gtgataaaaa tattcaagag ctcatattcc ggatcctttt tcggcgacca gataaaaata
     4801 ctcaagagca ggaaaattga caatgtaaat atgagagaca ccctctcggt gcggctcaag
     4861 gcaggattga gcacctgcct ggaactcgac ggggtaagaa accatttcga ttttatctcc
     4921 agggcaagca tgccggaata caggtacagc atcagcgata ttgtatccta tgaagatgaa
     4981 tcggcctatg tgatagattt cgaacagagg gaatacgcgg aacaggccct ctacaggggt
     5041 tcgctctata tcaatgccct ggattatgcc atcctgcatg ccggctttga agtcaatcct
     5101 aaatacctcg gcaggatgaa aagctccttc atctcgagca ccagcagggg ttatgacacc
     5161 tggccgcagt cggtgaaata tacagtaagc taccgcaggt acagcaacag gtatttcctc
     5221 aaccacgtga ggggcgacct ggttttcata tcaaaacaga aacggaaatt attcaacact
     5281 cagttcaggg tgttttttga actggcggtt accggtatcg agactgaagg agtcaccagg
     5341 ttcgaccgcg aggagcttgc tcccattcat tcagtatttt ccaggacaat aaaaagctat
     5401 gattcaaaat tctgggaaaa ccaggatttc ctcaagcctg aggaaaacct tcttcaggca
     5461 ctgaaaaaca tgaatgtccg cctcggggaa ttcaccgggg atgatcagta actgtcgatc
     5521 agattgtaca ggcctgtaag gtcgggtgta agaaccacat ccgtccttct gtttttctgc
     5581 cttgcatcgg atgtcttcct tgcatcaacc ggcataaact cactgtgacc ggcagcagta
     5641 aggcgcttcg gatcaatcct tgaaccttcc agcaataccc ttacaacggt tgtcgccctt
     5701 ttcacgctca gatcccagtt gtccctcagc tggctcccgg aagggttata gggcacatca
     5761 tcggtatggc cctctattgt tatctgtatt tcagggtttc tttcaagtac ggccgccagc
     5821 tttttcagtg cattcctgcc gtttgcatca atctcccagc ttgcactttt gaaaaggagc
     5881 tgctcttcca gcgaaacata taccttgccg tttttctggg ttaccgtaag gccgttgttc
     5941 tcgaagccga gcagggcttc agataccttg ttcttcagat cctgtgcaat ccttttctgt
     6001 gcgtcaagta tcctttccag ctccaccagc ctggcattcc gcttttcaag ctcataggtg
     6061 agctcatcaa gcgatgcctt ccttgtatca agcgtctcct ccagcctgcg gagcaggtct
     6121 tccttgttcc tgagatttgc ctgtgcggcc tgaagctcgg taagcagttt ctgcgtttcc
     6181 cttacattac ccctgataag ctcttcctgg gcattctgga gatcgttgta tcttcctgaa
     6241 atgaccctga attcctccag agccttgtcc ctttcctttt ccgcctttga aatatcttcc
     6301 ctcgtcttct gaacctccct gtccataagc gccattttgg cttcaagctc acgattttcc
     6361 atctccagat aaaggtttga acttttcagg tcatcccttt cattcatatt ctgcatgctc
     6421 gtaacctgaa gatcccggta cttttttgcc ggaacacagg agaaggacgc aatgagaaaa
     6481 atccataaaa gaggtttaat catttttccg ctcatgatcc gatattttta atcataaaac
     6541 tcctgacttc aataaaatat atccacacac tcatacagac agggcaaaaa taagaaaatc
     6601 ctgcctatgc ttcttcaaag cgcagggaat aataattgtt atatttgcaa actaaggcat
     6661 ttttttaaat gagccagggt aaagacctgt tattcaagat atcatctccc gaagatctga
     6721 ggaagctcaa cccttccgat ctcatcaggg tgagtgagga actgcggcag ttcatcattg
     6781 atgaagtcag taataacccg ggacatttcg gtgcaagtct cggcgttgtg gaactgacca
     6841 ttgcccttca ttatgttttt gacacgcccc gcgacaaaat tatctgggat gtcggacatc
     6901 aggcatacgg gcataagatc ctcaccggcc ggagggatgt tttttccaca aacaggaaat
     6961 acaggggcat atcgggtttc ccgcgcatga gtgaaagcga atatgacgct ttcggaacgg
     7021 gccattcctc aacgtccatc tcggccgcgc tcggaatggc ggtcgcatcc aggcagaaag
     7081 gggagaaaag gcatgtcgtt gccgtgatag gagacggggc catgaccgcc ggtgaagcct
     7141 ttgaaggact caacaatgcc ggtatagcca agtccgacct gctggttata ctgaatgaca
     7201 acaacattgc aatagatcag aatgtggggg cactgaagga atatctcctc gacatcgcca
     7261 ccagcggatt ctataacaaa ataaaggaca aggtgtggaa cctgctgggc agggccggaa
     7321 gattcggccc gaatgcccgt accctggccg cacagcttga agccggattc aaaagcactc
     7381 tcctcgacag gagcaacctg ttcgaaggaa tgaacttcag gtatttcgga cccattgacg
     7441 ggcatgatgt gatgcacctg gtgaaagttc tggaagacct gaagagaata agaggtccaa
     7501 agctgctgca ttgccttaca gtcaagggga aaggcttccg gcaggctgaa aggcaccaga
     7561 ccgaattcca tgcaccgggc aggtttgaca agcagacagg tgcaatactg agttcagatc
     7621 atgaggattc cgcccctccc taccaggagg tcttcggaca taccattctc gaactggcca
     7681 ggatgaatga aaagattgtc ggtgttactc ctgccatgcc aaccggctgc tcactgtgca
     7741 tcatgatgaa ggaaatgccc ggcagggctt tcgatgtcgg catagcggaa cagcatgctg
     7801 ttactttttc tgccggactg gctacacagg gactgatacc tttctgcaac atatattcct
     7861 cctttatcca gagagcctac gaccaggtta tccatgacgt tgcactccag aacctccgtg
     7921 ttgtcttctg tatcgaccgg ggcggccttg tgggctccga cggggcaaca caccacggct
     7981 ttttcgacct tgcattcatg cgcaccattc cgaatatgat tgttgccgct cccatgaatg
     8041 ccaccgagct gaggaacatg atgtacaccg cccagtcaga cagggtcagc tctccctttt
     8101 ccatccgcta tcccagggga tcaggcatgg taagggaatg gcggcggggc ttcagtgaaa
     8161 tcccggtcgg cagggcaaga ctgatcaggg aaggaagtga tattgccgtt ctgagcatag
     8221 gccatccggg caatgatgtg gataaagcag cgcggaaact ggaagctgaa tcagtttcag
     8281 tatgccactg ggacatgcgc tttgccgctc ccctcgatga agaagccctt cactcggtgt
     8341 tcggcaggtt taaaaagatc ataacggttg aggacggaat actgaaaggc ggattcggca
     8401 gtgcagtgat tgaattcatg agtgacaaca actacagtgc agagataagg cggctgggca
     8461 ttcccggcta tttcgtggaa cacggcacac aggaggagct ataccgtgaa tgcggctatg
     8521 atgccgtagg aattgaaaac acaataagaa agatgctgaa atagtatgtc cggaacctgg
     8581 caggatgcat aaaagactgt gaaccaggag gagatgccgg gttcatatta tcactgaaca
     8641 gaagatcgta aatcagtata tactaatcaa caatagaatc agtcatgaaa aaaacaatca
     8701 ttttaccggt gcttgttctg ctcgccgcat catgcggaac aaggacgaac aaggcagaca
     8761 atgaggaatg gattcagctt ttcaacggca gggatctcag cgggtggatt atcaagatca
     8821 agggatcgcc cgcgggcgtg aattacaaaa ataccttcag ggttgaagac gggataatga
     8881 aggtaagcta tgatgaatac gacaggttca atgccgaata cgggcatata ttctatgagc
     8941 agcccttttc gcgctacaag ctgaggatag aataccgttt caccggcaac caggtagcgg
     9001 gcggtcaggg ctgggcatac cgcaacagcg gggtcatgct ccattcacag tctcccgggt
     9061 caatgcttct
//
LOCUS       DAOSPX010000101         9069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7312_length_9069_cov_4.758931, whole genome shotgun sequence.
ACCESSION   DAOSPX010000101 DAOSPX010000000
VERSION     DAOSPX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9069
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7312_length_9069_cov_4.758931"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLZ75_06530"
     CDS             complement(<1..469)
                     /locus_tag="PLZ75_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS72091.1"
                     /translation="MTGKPVKIFLVEDDLSFGSVLKSYLELNDFLVDWIDDGKYAVDH
                     FRKGMFDICILDVMLPHVDGFTIANEIRQINNLVPIIFLTAKKMKEDVLKGYGVGGDD
                     YITKPFDTDILLAKLRAILARRDFQNGTKDIYELGKFTFNSKLRTLTTEDEEKK"
     gene            complement(466..2091)
                     /locus_tag="PLZ75_06535"
     CDS             complement(466..2091)
                     /locus_tag="PLZ75_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS72092.1"
                     /translation="MSRKKFIWLIFMMLVSLIGIIWVQSVWIRNAVGTQNENFDAAVI
                     AGINDAAGSIETARELDFFNNFMFSGSRAMNGLTDDISQYLSVDSYLSPAGEELSVRI
                     TNQAITRTPGQDPVVVVHDTVIKANGDSSIIFPSVRRSAIPGPDDKAGMIPGKESSYV
                     QQQEFIEWVKKRQNEFQNIGNRMISEIYSWEKSLALDDKEIEFALKRSLLFRNIQTPF
                     EFAVIRNGIIDEGRYKKTDVNDFIKSKYKVSLFPHSFIQQDLILSVIFPERTNYVLGS
                     MAWVLSASLLFSLIILATFAMSLSFIFRQKKISEMKSDFLNNMTHEFKTPIATISLAA
                     DTIVNSRVIGDESKIRHFISMIKKENSRMNKKVETILQISSLDRKEISFNFENLSLHS
                     IIERAVDSTDIIVQQRKGRVNLHLDASEPVVCGDEEHLYNLVNNLLDNAIKYSPGSPE
                     ITVSTKNNGRGVIMTVEDRGTGMSKSVQNRIFERFYRKPSGNVHNVKGFGLGLNYVQA
                     IVNAHRGNITVHSESGRGSRFDVFIPFNPENER"
     gene            complement(2229..2990)
                     /locus_tag="PLZ75_06540"
     CDS             complement(2229..2990)
                     /locus_tag="PLZ75_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOS72093.1"
                     /translation="MNEVFNDASESKKLNGPEVLYEDNHLIALNKKSSDLSQGDASGD
                     LSLDARVKQYLAAKYDKPGDVFLGVVHRLDRPVSGVILYARTSKALSRMNEMFRQSLV
                     KKTYLAIVGECPPEDEGVLIHYLRKNEKQNKSYVFDREVAGSRKAVLTYRIAGRSQRY
                     YLLEIGLHSGRHHQIRAQLAAIGCPVKGDLKYGYRRANENGGISLFARKLEFIHPVRK
                     VNTGIVAPFPEGDLWRKFNYDAGNPETEVKRKGQR"
     gene            3135..5063
                     /gene="dnaK"
                     /locus_tag="PLZ75_06545"
     CDS             3135..5063
                     /gene="dnaK"
                     /locus_tag="PLZ75_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOS72094.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVIIPNSEGKRTTPSIVAFVE
                     NNERKVGDPAKRQAITNATKTVYSIKRFMGETYDKLNPEIKRITYNVVKGDNNTPRVK
                     IDNRLYSPQEISAMILQKMKKTAEDYLGQEVTEAVITVPAYFSDSQRQATKEAGEIAG
                     LTVKRIINEPTAASLAYGLDKKSQDLKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDHQIIDWLAEEFIRDEGVDLRKDPMAMQRLKEAAEKAKIELSSTTSTEIN
                     LPYIMPVDGIPKHLVKTLTRANFEKICDPLFQKCIQPVKKALSDSGYSVSDIDEVLLV
                     GGSTRIPAIQQLVENFFGKAPSKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLS
                     LGIETMGGVMTKLIDANTTIPTKKMEVFTTAADNQPSVEIHVLQGERPMATGNKTIGR
                     FHLDGIPPAPRGIPQIEVTFDIDANGILHVTAKDRGTGKEQRIRIEASSGLSDDEIRR
                     MREEAKANEEADAKARERVDKINSADSMIFSTEKQLKEFGDKLPADKKAEIESALEKL
                     KEAHKNQDVNAIDTSLASLNSIWQSASEEMYKTAQSTAPGGSSQHGQEHAGGQHHSGG
                     DSKSGNDEVTDVDFEEVD"
     gene            5195..5983
                     /locus_tag="PLZ75_06550"
     CDS             5195..5983
                     /locus_tag="PLZ75_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72095.1"
                     /translation="MNLKLFTITILLACAPILSGQDGSGLNKTDSEGRKQGQWTVRYS
                     NGNILYEGFFRDDYPEGEFRRYYEDQDINSVMIFSDKGRKAEATIYYPGGSIAARGIY
                     VNRMKEGRWKFYSSSIPGYLINEEEYAGNMKNGLSLKFYSDSSIAEKVTYRNDKKEGE
                     WIQYYASGKIFLKSSYSGGMLNGKFEVWYENGKPEISGEYRNNLREGRWIIYNEDGTE
                     RYEQNYTLGMTNDRQMDIDAAEIIDNMEKNRGKIADPQNTGEIR"
     gene            6054..7637
                     /locus_tag="PLZ75_06555"
     CDS             6054..7637
                     /locus_tag="PLZ75_06555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOS72096.1"
                     /translation="MLLNGQDISYKYFYRVWFTDKGSNTSITDPRELLSERAISRRVR
                     AGIEFPDFRDLPVYRGYIEAVEAGGLRLHCTSKWLNTALFKSSGPADISMVEDLPFVK
                     EVTPVKYPAGKSIFTDKLDFSAEQSDFPPHDHPLRMINGNLLKDIGYDGKGILIAVND
                     GGFRFTDQISSLEHLRRRNGIKVVRDFVSGDEFTYDHHTHGTAVMSILAGQLEGMLQG
                     AATGADYMLFRTEDVSGEFPAEEDYWAAAAEYADSAGADIITSSLGYGLFDDPSLNYK
                     FSDLDGNTAFITRAADIAASRGILVFSSAGNERNKTWKRILAPADGDSVVTVAAVDRD
                     RKISAFSSAGPSADGRIKPDLSAMGVNVPVQISPGSVSRSSGTSFSCPVLSGISACLL
                     QAVPEAAAPEIAGALRKSGDRYNSPDSLYGYGIPDINRALDILQETYLRRPGNAIVIY
                     PNPVREDFDIVFREYPESVILEIFSFSGKLVYRQDFSSSPGRLLKVRALAKREQGIYF
                     LRIRTANAMYLKKIIKLNS"
     gene            7640..9025
                     /gene="xseA"
                     /locus_tag="PLZ75_06560"
     CDS             7640..9025
                     /gene="xseA"
                     /locus_tag="PLZ75_06560"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOS72097.1"
                     /translation="MNEKLSLTELQLIIRDSLHLALPDFYWVVAEISEIKENYAGHCY
                     LELVEKQAGERNVRARARAIIWNTRYRLLKSLFENVTGESLESGMKILIRVRVDYSEI
                     YGLSLIVSDIDPSYTVGEMAVKRQMILKRLEEEGVITMNKEHEFPLVPQRIAVISSGS
                     AAGWSDFSRHLRENHYGYVFYTALFEAVMQGTETERSIIGAFERIAENIGHFDLVAII
                     RGGGSQSDLSWFDNYNIAYYVTQFPIPVITGIGHEKDVSVTDIVAFKALRTPTAVADF
                     LINSVAEAETQLTELGSRIADKSRGIIAEHKDITTTFLSKLMPLAKLAVADQKERLSN
                     RIMGILNIGKEYLTRAGFIPENLKNRLNSSSASFILSKRTYLEKNRMDLMNYSVKIIE
                     NQKMKLSALDNSLDILNPVNVLKRGYSLTSINGVIIRSVKDAEKNDVLETRLQDGKIK
                     SRVTGKQYLKD"
BASE COUNT         2660 a         2081 c         2032 g         2296 t
ORIGIN      
        1 gctttttttc ctcatcttcc gttgtaaggg ttcttagttt tgagttgaag gtgaattttc
       61 ccagttcata aatatctttt gtaccatttt gaaaatcacg gcgggcaaga attgccctga
      121 gtttcgcaag gagaatatca gtatcgaatg gttttgttat gtagtcatcc cctcccactc
      181 catatccctt cagaacatcc tccttcatct tttttgccgt caggaatatg ataggcacaa
      241 ggttgttaat ctgccttatt tcatttgcta tggtaaatcc gtcaacatga ggaagcataa
      301 catcgagaat gcagatatcg aacatgcctt ttctgaaatg atcaactgca tatttcccgt
      361 catcaatcca gtcgaccagg aaatcattca gttctagata tgatttcaga accgatccga
      421 aactcaggtc atcctcaacc aggaatatct taaccggttt tcccgtcatc tctcattttc
      481 cggattaaaa ggtataaaaa catcaaaacg gcttcccctg cccgattcgc tgtgaaccgt
      541 aatatttccc ctgtgggcat tgactatggc ctgcacataa ttgagtccaa ggccgaatcc
      601 cttgacatta tgcacatttc cggaaggttt cctgtaaaat ctctcaaata tcctgttctg
      661 aacacttttc gacatgcctg tccccctgtc ttccaccgtc ataatgaccc ctctgccgtt
      721 gttttttgtg cttacggtta tttccggaga tcccggcgaa tatttgatgg cattgtcaag
      781 aagattgttt acaagattgt aaagatgctc ctcatcaccg cataccacgg gttcagatgc
      841 atccagatga agatttaccc tgcccttcct ctgctgcaca atgatgtcag tcgaatccac
      901 ggcacgttca ataatcgaat gcagggacag attctcaaaa ttaaagctga tctccttcct
      961 gtcgagcgat gatatctgaa gaatggtctc caccttttta ttcatgcggc tgttttcctt
     1021 ttttatcatg ctgatgaagt gacggatctt cgattcatca ccaatgaccc tggaatttac
     1081 gattgtatca gctgcaagcg atattgtggc aatcggtgtc ttgaactcat gggtcatatt
     1141 gttcaggaaa tccgatttca tttcagaaat ctttttctgc ctgaaaataa atgaaaggct
     1201 cattgcgaaa gtagcaagaa tgatcagtga aaaaagcagg gatgccgaca gaacccatgc
     1261 catcgagccc aggacataat tcgtgcgttc cggaaagatc accgacagga tcagatcctg
     1321 ctgaataaaa ctatgcggga aaagactgac tttgtattta cttttaatga aatcgttaac
     1381 atctgttttt ttatagcgtc cctcgtctat aattccgttt ctgataacgg caaattcaaa
     1441 gggtgtctgt atgtttctga aaagaagtga tcttttaagg gcaaactcta tctccttatc
     1501 atcaagtgcc agtgattttt cccaggagta gatttcagaa atcatcctgt tgcctatgtt
     1561 ctggaattca ttctgcctct tttttaccca ttcgatgaac tcctgctgct gaacatatga
     1621 gctttccttt ccgggaatca tacctgcttt atcatcaggt cccggaatgg ctgatctcct
     1681 gactgaaggg aatataatag aggaatcccc gtttgccttg attaccgtat catgaacaac
     1741 aacaacggga tcctgcccgg gagtcctggt tattgcctga tttgttatcc tcacactcag
     1801 ctcctcaccg gccggggata aatagctgtc cacactcaga tactgtgaaa tatcatcagt
     1861 cagaccgttc attgccctgc ttccgctgaa catgaaatta ttaaagaaat ccagctccct
     1921 ggctgtttct atggaaccgg cagcatcatt tatacctgca atgacagcag catcgaagtt
     1981 ttcattctga gtgccgacgg catttctgat ccagaccgac tgaacccata tgataccgat
     2041 aagtgaaacc agcatcataa atatcagcca tatgaatttc ttcctgctca tgcggcaaaa
     2101 ttaccttatc cctgcgtatc ttacactaaa tttaactttt ccttaacggt tattaacctg
     2161 aatttaactt cactcctgcc tttcatgtga aaaatcgggg cctcaagttc aatcccggtt
     2221 ttttccggtc agcgctgccc ctttctttta acttcagttt caggattccc cgcatcataa
     2281 ttgaactttc tccataaatc tccttcaggg aagggagcaa ctatcccggt gtttaccttt
     2341 ctgaccgggt gaataaactc cagtttcctg gcaaacaggc ttatccctcc gttttcattc
     2401 gccctcctgt aaccgtattt cagatcaccc ttcaccgggc agccaattgc agcaagctgt
     2461 gccctgatct gatgatgccg tccggaatgc agtccgatct caagaagata atatctctgg
     2521 gaccgtccgg caatcctgta ggtcagtacc gcctttctgg aaccggcaac ctccctgtca
     2581 aagacatagg acttgttctg cttctcattc ttcctgagat agtgtatcag tactccctca
     2641 tcctccggag gacattcccc tacaattgcc aggtaggtct tttttaccag ggattgcctg
     2701 aacatctcat tcatccgcga cagggccttt gaggtacggg catagaggat gacaccgctg
     2761 acaggcctgt caagcctgtg gacaacacca agaaaaacat ccccgggctt gtcatatttt
     2821 gcagcaaggt attgcttcac ccttgcatca agggaaagat cgcccgaagc atcgccctgc
     2881 gataaatcgg aagacttctt gttcagggca attaaatggt tatcctcgta aagaacttcc
     2941 ggcccgttta gttttttact ttctgacgca tcattaaaaa cctcgttcat agtgcaaaat
     3001 tactcttttc tttttattca gcctcctggt ccggcctgtg ccggctactg tcgttttgtc
     3061 agttatttca aatggcataa tcattgatga acatagtatc aaagtaaaaa atcgataatg
     3121 caaaaattta taaaatggga aagataatcg gtatagatct tggtacaaca aattcatgtg
     3181 ttgcagtaat ggaaggcaat gagcctgtta ttatacccaa cagtgaaggc aaaagaacca
     3241 caccttcaat agttgccttt gttgagaata atgaacgcaa agtcggtgat ccggcaaaac
     3301 gtcaggctat aacaaatgcc acaaagaccg tgtattcaat aaaaaggttc atgggcgaga
     3361 cctatgacaa actgaatccg gaaataaaaa gaatcacata taatgtagtc aagggtgaca
     3421 ataatactcc cagggtaaaa atcgacaaca ggttgtattc accccaggag atttctgcaa
     3481 tgatacttca gaagatgaaa aagaccgctg aggattatct gggccaggaa gttactgaag
     3541 cagttataac cgtaccggcc tatttcagtg attcccagcg acaggccacc aaagaagcag
     3601 gagagatcgc cggtctgact gtcaagagga taatcaatga accgacagca gcatctctcg
     3661 cctatggtct tgataaaaaa tcacaggatc ttaaaattgc ggtatttgac ctgggtggag
     3721 gaacattcga catatccata cttgaactgg gagacggtgt ttttgaggtt aagtcaacca
     3781 atggcgacac ccatcttggc ggagacgatt ttgaccatca gataatcgac tggcttgctg
     3841 aagaatttat cagggacgaa ggggttgacc tgaggaaaga tcccatggca atgcagagac
     3901 tgaaggaagc tgccgaaaag gcgaaaatag aactttcaag cactacttca acagaaataa
     3961 atcttcctta tataatgccc gtcgacggaa ttccgaaaca tcttgtcaaa acccttaccc
     4021 gggcaaattt tgagaagata tgcgatcccc tattccagaa atgtattcag ccggtcaaaa
     4081 aagccctgtc tgattcgggc tactcagtat cggatattga tgaagtcctt ctggtgggtg
     4141 gttcaacaag aattccggca atacagcagc ttgtggaaaa tttcttcgga aaagctcctt
     4201 caaaaggcgt taatcctgat gaagttgttg ctgttggtgc tgcaattcag ggtggtgtcc
     4261 tcacgggcga ggtgaaggat gtgcttctcc ttgatgtcac cccgctttcg ctgggtattg
     4321 aaaccatggg cggtgtcatg acaaaactca ttgatgccaa tacaacaata ccaaccaaga
     4381 agatggaagt cttcacaacc gcagccgata atcagccttc ggttgagata catgtactgc
     4441 agggcgaaag gccaatggcg acaggaaaca aaacaatcgg aagattccat ctcgatggca
     4501 ttccgcctgc tccgaggggc attccgcaga tagaggtcac tttcgatatt gatgcaaacg
     4561 gaatcctcca tgttaccgca aaagaccgcg gaacgggaaa agaacaaagg ataaggattg
     4621 aagcctcgtc cgggctcagc gatgatgaaa tcagacggat gcgtgaagag gcaaaggcca
     4681 atgaggaggc tgacgccaag gcaagggaaa gagttgacaa gatcaattcg gccgacagca
     4741 tgatattcag tactgaaaaa cagttgaaag agttcggcga caaacttcct gccgataaaa
     4801 aagctgaaat agaaagtgcc cttgagaaac tgaaagaagc tcataaaaat caggatgtaa
     4861 atgcaataga tacgtcactc gcaagtctga acagcatctg gcagtctgct tcggaagaaa
     4921 tgtacaaaac agcacagagc acagcacccg ggggttcatc acagcatggc caggaacatg
     4981 cgggcggtca gcatcacagt ggcggcgaca gcaaatccgg caacgacgag gtcacagacg
     5041 tggattttga agaggttgac taacacctgt ttttcaggtc actgttaaac tataagcgcg
     5101 ggaaggcaaa tactttgttt tcccgcgttt tattattttt tctttcagac ctccaaattt
     5161 tgtactttta cggtagattt tccgtttctt tcatatgaat cttaagttat tcacaatcac
     5221 aatactattg gcatgtgccc cgattctatc tggccaggac ggcagcggcc ttaacaaaac
     5281 cgattctgaa ggcagaaaac agggccagtg gacggtaaga tattccaacg gaaacatcct
     5341 ttatgagggt tttttcaggg atgattatcc cgaaggggaa ttcagacgat attatgaaga
     5401 ccaggacatc aactcggtaa tgatattctc cgataaaggc aggaaggccg aagcaaccat
     5461 ctattacccc ggaggctcaa tcgctgcaag aggcatctat gtgaaccgga tgaaagaagg
     5521 caggtggaaa ttttattcat cctccatccc cggttatctc atcaatgagg aagaatatgc
     5581 gggaaacatg aaaaacggac tatccctcaa attctatagt gacagcagca tagccgaaaa
     5641 agtaacttac aggaatgaca aaaaggaagg cgaatggata caatattacg ccagcggtaa
     5701 aatattcctg aaatccagct attcgggcgg catgctcaac ggaaaatttg aagtgtggta
     5761 tgaaaacgga aagcctgaaa taagcggaga atacaggaat aatctccgtg agggcagatg
     5821 gatcatttac aatgaggacg gaacagagag atatgaacag aattataccc tgggcatgac
     5881 aaatgaccgt cagatggata ttgatgccgc tgaaataatt gacaatatgg aaaagaacag
     5941 gggaaaaatt gctgatccgc aaaataccgg agaaatcaga tgaaccattg aaacagcagt
     6001 cattccgtat caaattgaaa aaaattcccg ccgtcatatt actgctttcc ggattgcttc
     6061 tcaatggaca ggatatttca tacaagtatt tctacagggt ctggtttacc gacaaaggat
     6121 caaacacctc cataacggat ccccgcgaac tgctttccga acgggcaatc agcaggaggg
     6181 tcagggcagg cattgagttt cccgacttcc gggaccttcc cgtttaccgc ggttacatcg
     6241 aagctgttga agccgggggc ctcagattgc attgcacctc aaaatggctg aacaccgcac
     6301 ttttcaaatc ttccggacca gccgacatca gtatggtcga agacctgccc tttgtaaagg
     6361 aagtcacgcc ggtcaaatac cctgcgggaa aaagcatctt taccgacaag ctggatttct
     6421 ccgcggaaca atccgatttt ccgccccatg atcatcccct caggatgatc aacggaaacc
     6481 tgcttaaaga cataggctat gacggcaagg gaatccttat cgcagtgaat gacgggggct
     6541 tcagattcac agaccagata tcttccctgg aacatctgcg cagacgaaac ggaataaagg
     6601 ttgttcgcga ttttgtcagc ggagatgaat tcacttatga ccatcataca cacggaaccg
     6661 cggttatgtc aatacttgcc ggacaactgg agggaatgct gcagggtgcc gcaaccggcg
     6721 ctgattatat gcttttccgt acagaggatg tttccggtga atttcctgca gaggaggatt
     6781 actgggccgc ggccgcagaa tatgctgaca gtgccggtgc agatatcata acctcatccc
     6841 tgggatacgg cctttttgat gatccctcgc ttaattacaa gttcagcgat cttgacggaa
     6901 acactgcctt tataacaagg gcagcagata tcgcagcctc caggggaata ctggttttca
     6961 gcagtgcggg aaatgaaaga aacaaaacct ggaagagaat cctcgctcct gctgacggcg
     7021 acagtgtcgt gaccgtggct gcagtcgacc gggacaggaa aatctcagcc ttctcgtcgg
     7081 caggcccttc agccgacgga agaataaaac cagatctttc tgccatggga gtgaacgtac
     7141 ccgtacagat atcaccgggt tctgtatcaa ggtcaagcgg tacatccttt tcatgtccgg
     7201 tactcagcgg aatcagcgcc tgccttctcc aggcagtacc tgaagctgca gcccccgaaa
     7261 tagccggtgc actccgtaag tcaggggaca gatataactc ccccgattca ctttacggct
     7321 atggaatacc cgatataaac cgggctcttg atatattgca ggagacttac ctcagaaggc
     7381 ccggaaatgc gattgtcatc tatccaaatc ccgtcaggga agattttgac attgttttca
     7441 gggaataccc ggaatcagtg atcctggaaa tcttcagctt ttcagggaaa cttgtttacc
     7501 ggcaggattt cagttcgtct ccgggaaggt tgctgaaagt cagggctctt gcaaaaaggg
     7561 aacagggaat atatttcctc agaatacgga cggcaaatgc catgtattta aaaaaaatca
     7621 taaaactgaa cagctgacaa tgaatgaaaa attatctctt actgaattgc agctgataat
     7681 cagggactcc cttcatctgg cattgcctga tttttattgg gttgttgccg aaatatctga
     7741 aataaaggaa aactatgccg gccattgcta cctggaactt gtcgagaaac aggccggtga
     7801 aaggaacgta agggcaaggg ccagggcaat aatctggaat acccggtaca ggctgctaaa
     7861 atcattgttt gaaaatgtaa cgggagagtc actggaaagc ggaatgaaaa tcctgatccg
     7921 tgtaagggtc gattacagtg aaatatatgg cctgagcctc atcgtttcgg atatagaccc
     7981 ctcctacacg gtaggggaaa tggcggtaaa gagacaaatg atcctgaaaa ggcttgaaga
     8041 agagggagta atcaccatga ataaagaaca tgaattcccc cttgttccac agagaatcgc
     8101 agtcatttcg tcgggaagcg cggcaggctg gtcggatttc agcaggcatc tcagggaaaa
     8161 ccattacgga tatgtcttct ataccgcact tttcgaagcc gtaatgcagg gaacagaaac
     8221 cgaacgcagc attatcggcg cttttgaaag gatagctgaa aacataggcc actttgatct
     8281 tgtggcaata atcaggggcg gaggctcaca gagcgatctc agctggttcg ataattacaa
     8341 tattgcctat tatgttaccc agttcccgat tccggtaata acaggcatcg gtcatgaaaa
     8401 agatgtttca gtaacggata tagttgcttt caaggcactc cggacaccga cggcagttgc
     8461 ggattttctc ataaactccg ttgctgaggc agaaacacag ctgacggaac tcggcagccg
     8521 gattgcagat aaatcccgag ggataattgc tgaacacaag gatatcacca ctacattcct
     8581 gtcaaagctc atgcccctgg caaaactggc tgtcgctgac cagaaggaac ggctctcgaa
     8641 ccggataatg ggaatactga atatcggaaa agaatacctg accagggcag gattcattcc
     8701 ggaaaacctt aaaaacaggc ttaactcctc ttctgcttcc ttcatacttt caaaaagaac
     8761 atacctggaa aaaaacagga tggacctgat gaattactct gtcaaaatca ttgagaacca
     8821 gaaaatgaaa ctgagcgctc tggataattc actggatatc ctcaacccgg tcaacgttct
     8881 taagagaggt tattcactga ccagcattaa cggagtgata atcagaagtg tgaaggatgc
     8941 tgaaaaaaat gacgtcctgg aaacacgcct gcaggacgga aaaataaaaa gcagggttac
     9001 gggcaaacaa tatctaaaag actaaataca atggcaaaaa aggaattctc attcaccgaa
     9061 gcggtaaag
//
LOCUS       DAOSPX010000102         9049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7326_length_9049_cov_3.756727, whole genome shotgun sequence.
ACCESSION   DAOSPX010000102 DAOSPX010000000
VERSION     DAOSPX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9049
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7326_length_9049_cov_3.756727"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLZ75_06565"
     CDS             complement(<1..355)
                     /locus_tag="PLZ75_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOS72098.1"
                     /translation="MKSYRRELWFETPARRAFINITGQVEKCLEESGIREGIIVVSAM
                     HITSSVFINDDESGLHHDLEVWLEKLAPEKPHSQYRHNSFEDNADAHLKRSIMGREAV
                     VAVSSGKLDFGPWEQI"
     gene            complement(473..2206)
                     /locus_tag="PLZ75_06570"
     CDS             complement(473..2206)
                     /locus_tag="PLZ75_06570"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOS72099.1"
                     /translation="MLSIEEIRKRAGIWLGSEFNEATRREVREMLEKDEKKLVDSFYQ
                     DLEFGTGGLRGIMGAGTNRMNIYTMGMATQGLSNYIIKQCGGKGIRVAIAHDCRNNSR
                     YFAETAADIFSANGFEVFLFEALRPTPELSFAIRHYKCQSGIVITASHNPPEYNGYKA
                     YWNDGGQVVAPHDSGIIEEVRRIKSVNEIKFGGNKQNIRIIGKETDDAFLEEVLKMSL
                     NPEIIREHSDMGIVYTPIHGTGVRLVPSALKLFGFTNIINVPEQDIVDGNFPTVRSPN
                     PEEPDALKMAIDKAETTGAELVIATDPDADRLGIACRNREGEFILLNGNQTAVLLIHY
                     ILSQYGEKDKYKGNEYIVKTIVTSDLIDKIAEKHKVECFNVLTGFKFFAELMRKLEGK
                     KKYIGGGEESYGFLPGDYVRDKDAVASCALVAEIAAWARSKGKSLYDLLLDIYLEYGL
                     FLEKLVNIVRKGKEGADEIKAMMMSYRNNPPEIINKSRVVKIDDYESLVSTDCLTGKK
                     TNLNLLRSDVLQFFLEDGSKISVRPSGTEPKIKFYFSVNTALESAEKFEETILLLDRR
                     IEGIITDMKLN"
     gene            complement(2300..3166)
                     /gene="rfbD"
                     /locus_tag="PLZ75_06575"
     CDS             complement(2300..3166)
                     /gene="rfbD"
                     /locus_tag="PLZ75_06575"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOS72100.1"
                     /translation="MAVILITGAGGQLGSELRTASGNYYGYDFIFTDIDTLDIGDKAK
                     TSDFIRNTNPDWIINCAAYNQVDKAEEDSATADLVNRAAVSHIAEAIRSTECRFIHFS
                     TDYVFSGMSCQPYNEFSEPGPINAYGMSKLAGEKAALTHHASMIIRTSWLYSSFGTNF
                     VKTILRKARENESLNVVFDQVGTPTYAADLAEAVLAIVSGVIRNKTTYSPGIYHYSNE
                     GVCSWYDFAEEVVREAGLACRIVPVLSSRYPMLAQRPSYSVLDKGKIKEKYGLAIPHW
                     RSSMKKCLGLLV"
     gene            complement(3182..3730)
                     /gene="rfbC"
                     /locus_tag="PLZ75_06580"
     CDS             complement(3182..3730)
                     /gene="rfbC"
                     /locus_tag="PLZ75_06580"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOS72101.1"
                     /translation="MEIIHTGFKDLFVIKPTVYSDKRGYFFESYNQDTYRNAGIHFMP
                     IQDNESRSSKGVIRGLHYQLRPYDQAKLIRVIEGRIFDVALDLRKDSPTYGKWYGIEL
                     DSGSKTQLFIPRGFAHGFSVLSELAIILYKTDNLYNNASERGIALDDPDLGIDWHIGN
                     IKPILSEKDTRNPLFRDADNNF"
     gene            complement(3745..4638)
                     /gene="rfbA"
                     /locus_tag="PLZ75_06585"
     CDS             complement(3745..4638)
                     /gene="rfbA"
                     /locus_tag="PLZ75_06585"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOS72102.1"
                     /translation="MKGIILAGGSGSRLHPITNTISKQLLPVYDKPMIYYPLSVLMLA
                     GIREILVISTPRDLPSFRNLLGDGKSIGLEFSYIEQPSPDGLAQAFILGDEFIGNDPV
                     CMILGDNIFYGHGFGDVLRNTARLDDGACVFGYYVTDPERYGVVEFDSSGRAVTIEEK
                     PARPKSNYAVTGLYFYDNTVVSKARSLKPSPRGELEITDLNRLYLEEGKLTVRIMGRG
                     MAWLDTGTFESLLQASNFIATLEHRQSLKASCIEEIAYKRGFIDKNQLLSLAEPIKNS
                     QYGKYLMRIASEEISGPDIMK"
     gene            complement(4674..6008)
                     /locus_tag="PLZ75_06590"
     CDS             complement(4674..6008)
                     /locus_tag="PLZ75_06590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOS72103.1"
                     /translation="MKISVIGTGYVGLVTGTCFAETGVTVTCIDRDTDKIRGLNKGTI
                     PIYEPGLETMISRNVEKGRLFFSSSVSEGIEGSEVIFIAVGTPPGEDGSADLKHVLEV
                     AREIGDSLKDYALIVTKSTVPVGTSEKVRETIQRQLEKRQSGIRFSMASNPEFLKEGA
                     ALEDFLRPERIVVGVDNPEAGEVMQRLYKPFVLNNHPIIFMDIPSAEITKYAANAMLA
                     TRISFINEMANLCDLLGADINHVRRGIGSDSRIGSKFLYPGPGYGGSCFPKDVKAIIK
                     TAEELGYELNVVKAVEKANEFQKNIIFNKMKYHFSGRLEKKTIGIWGLSFKPKTDDVR
                     ESSSLHLIRKLLDKGAVVRAYDPAAMKETKKLLGDSIQYCDNPYEALENTDAMALMTE
                     WPEFQLPNFKKMAGIMKDRVIFDGRNIYDPALLVKMGFKYYGIGRRPQEEIK"
     gene            6340..8466
                     /gene="rpsA"
                     /locus_tag="PLZ75_06595"
     CDS             6340..8466
                     /gene="rpsA"
                     /locus_tag="PLZ75_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOS72104.1"
                     /translation="MTDTRKKNPKKDDLADSEIVKSAKKLKIKESKDKLLEQAFEEDI
                     IPGSQFDITDSDDDPVKSETDDLTEFIHGSESGGEEENAGDFQETVETVAESGDLTAD
                     ESDIPEIAEISAGKPSVEDFDWESFESEEAKLSPRHKEYEAMYDKTLSTVAVDEVISG
                     TVIQMTSREVVINIGYKSEGVVSLNEFRYNPGLKVGDEVEVYVESQEDKKGQLILSHK
                     KARAIKSWERVNASLENDEIITGYIKCRTKGGMIVDVFGIEAFLPGSQIDVKPIRDYD
                     IYVGKTMEFKVVKINQEFKNVVVSHKALIEAELEQQKKEIIAKLEKGQVLEGTVKNIT
                     SYGVFIDLGGVDGLIHITDLSWGRVNHPEEIVELDQKLNVVILDFDDDKKRIALGLKQ
                     LTPHPWDSVDPDLKVGDRVTGKVVVMADYGAFVEIAPGVEGLIHVSEMSWSQHLRSAQ
                     EFMKVGDEVETVILTLDRAERKMSLGIKQLKPDPWQDIEQKYTVGSRHTARVRNFTNF
                     GVFVEIEEGVDGLIHISDLSWTKKIKHPAEFTAIDAEIEVVVLEIDKENRRLSLGHKQ
                     LEENPWDVFEDLFATDSIHEGTVMEVFDKGAIIALPYGVEGFATPKHLVKEDGSVAKV
                     DERLMFKVIEFNKSAKKIIVSHSRVYEDEKKAAEAPEKKSEPETPRKSARKAKTNIER
                     TTLGDITQLAALKEEMEEKENKASKE"
     gene            8506..8844
                     /locus_tag="PLZ75_06600"
     CDS             8506..8844
                     /locus_tag="PLZ75_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOS72105.1"
                     /translation="MDFNIESRNNSTLIRIQTEKLDTHIAPALKSELVLISGKGEKNI
                     ILDLEKTQYCDSSGLSAILVANRLCKNAGGTFVLCGLNEAVERLITISQLDTVLTITE
                     TVADAEKMIS"
     gene            8869..>9049
                     /locus_tag="PLZ75_06605"
     CDS             8869..>9049
                     /locus_tag="PLZ75_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOS72106.1"
                     /translation="MKLTILGSSSALPTSERYPSAHVLSVHERLYLIDCGEGTQMQLR
                     RNKIRFGKINHIFISH"
BASE COUNT         2348 a         2014 c         2003 g         2684 t
ORIGIN      
        1 aaatttgttc ccacgggccg aaatccagtt ttccgctgct cacagccaca acggcctcac
       61 ggcccataat gctgcgtttt aaatgagcat cggcattatc ctcaaaactg ttatgcctgt
      121 actggctgtg tggtttctct ggtgccagct tttccagcca tacctcaaga tcgtggtgca
      181 atcccgactc atcatcattg atgaacacag agctggtgat gtgcattgcg ctgacaacaa
      241 tgattccttc cctgatccct gattcttcca gacatttttc cacctgaccc gtgatgttga
      301 taaatgccct tcttgccggt gtttcgaacc agagttccct gcgatatgat ttcatgctta
      361 ctgttgtttg tttgtttatt tcctgatcag atctgtaaaa aatagttccg gcatttttta
      421 ccgggctcag tccaatttcc aaaatcccca tcgtaactga tgccacgcag gatcagttca
      481 atttcatatc agtgattatg ccttcaattc ttctgtcaag aagcaggatc gtttcttcga
      541 atttttccgc agattcaagc gcagtgttca cactgaaata aaatttgatt ttcggttctg
      601 ttccggaggg acgcactgaa attttacttc cgtcctccag aaaaaactgg agcacatccg
      661 acctcagcag attcagattt gttttcttgc ctgtcagaca atcagttgaa acaagcgact
      721 cgtaatcatc aatttttaca accctgcttt tgttaattat ttcaggagga ttgtttctgt
      781 acgacatcat catggctttt atttcatcag ctccttcctt gcccttcctg acaatgttga
      841 caagtttttc caggaataat ccgtattcga gatatatatc cagcaggaga tcgtacagtg
      901 atttcccctt gctccttgcc catgcagcaa tctcggcaac cagtgcacat gatgcaactg
      961 catccttgtc ccttacataa tcgccgggca gaaagccata gctttcctca ccgcctccga
     1021 tgtatttttt ctttccttcg agcttcctca tcagttcagc gaagaacttg aatccggtca
     1081 ggacgttgaa gcattcaacc ttgtgttttt cagcaatctt gtcaatcagg tcgctcgtaa
     1141 ctatggtttt gacaatatat tcattgccct tatacttatc cttttccccg tattgcgaca
     1201 gtatgtaatg gatcagcagt acggcagtct gattcccgtt cagaagaata aactcacctt
     1261 ccctgtttct gcatgcaatt ccaagtctgt cagcatcggg atccgttgca ataacaagct
     1321 ctgcccctgt ggtttctgct ttgtcaatgg ccattttcag tgcatctggt tcttcaggat
     1381 tgggtgatct gacagtgggg aaattcccgt caacgatatc ctgttccgga acattgatga
     1441 tattggtaaa accaaacagc ttaagggctg atggtaccag cctgactcct gttccgtgaa
     1501 tgggtgtgta aactataccc atatcactgt gttctctgat gatttccggg ttaaggctca
     1561 ttttaaggac ttcctcaaga aatgcatcat cggtttcctt tccgataatc cggatattct
     1621 gcttgtttcc cccgaatttt atttcgttta ctgattttat tctgcgaact tcctcaatta
     1681 taccggaatc gtggggtgcc acaacctgtc cgccatcgtt ccagtaggcc ttgtaaccat
     1741 tatattccgg aggattatgt gaagccgtta tcacaatccc gctctgacat ttgtaatgac
     1801 ggattgcgaa cgacagttcg ggtgtgggtc tgagggcctc gaaaaggaaa acctcaaaac
     1861 cgttggccga gaatatgtcc gcagccgttt cggcaaaata gcggctgttg ttcctgcagt
     1921 cgtgggcgat tgcgacgcgt attcccttac cgccgcattg ttttattata tagtttgata
     1981 gaccctgggt tgccattccc atggtataaa tattcattct gttggttccg gcacccatga
     2041 ttccccttaa tcctccggtt ccgaattcca ggtcctggta gaaggaatcc accagtttct
     2101 tttcatcttt ttcaagcatt tcccttactt ccctgcgggt tgcttcattg aattcactgc
     2161 caagccatat tccggccctc ttcctgattt cttcaattga taacatgtga aaggttattt
     2221 gatttctgtt gtaataataa gtaaattttc ctgaatgatc cgcctgtttt tgcatccgct
     2281 tactgcggaa gtgttatttt tatacaagta atcccaggca tttcttcata ctgctcctcc
     2341 agtgggggat ggcaagacca tatttttcct ttatttttcc cttgtcaagt acggaatatg
     2401 aaggtctttg tgcaagcatg ggataccggg aggaaaggac cggaacgatg cggcatgcca
     2461 ggccggcttc cctgaccact tcctcagcaa aatcgtacca gctgcagaca ccttcgtttg
     2521 aataatggta tatgccggga ctgtaagtgg tcttgttgcg tattactcct gagacaatag
     2581 ccaggaccgc ttctgccagg tcggctgcat aggtgggtgt cccaacctgg tcaaacacaa
     2641 cgttgagtga ttcattttcc cgcgccttcc tcagaatggt tttgacaaaa tttgttccga
     2701 aagatgaata cagccaggat gtccggataa tcattgaagc atgatgagtc agcgctgctt
     2761 tttcacctgc cagttttgac atgccgtagg catttatggg accgggttct gaaaattcat
     2821 tatagggctg gcaggacatg ccgctgaaaa catagtccgt tgaaaaatga atgaatctgc
     2881 attcagtgct tctgattgct tccgcaatat ggcttacagc ggctctgttt acgagatcag
     2941 ccgtggctga atcctcttca gccttgtcta cctgattgta ggccgcacag tttattatcc
     3001 agtcgggatt ggtatttctg atgaaatcac ttgtttttgc cttatcgcct atatcgagtg
     3061 tatcgatatc cgtgaagata aagtcatatc cgtagtaatt tccggatgca gttctcagtt
     3121 cactgcccag ctggccgccg gccccggtaa tcagtattac tgccatgaaa cttaagatca
     3181 gttagaaatt attatccgca tccctgaaaa ggggatttct tgtatccttt tcactcagta
     3241 tcggtttgat attcccgata tgccagtcaa tgcccaggtc gggatcatca agcgctatcc
     3301 ccctttctga agcattattg tagagattgt cggttttata cagaatgatt gcaagctcgc
     3361 ttaatacgga gaagccatgg gcaaagcccc ttggaatgaa aagctgtgtc ttgcttcccg
     3421 aatcaagttc aatgccatac cattttccgt atgttggtga atcctttctc aggtcgaggg
     3481 caacgtcaaa aatccttcct tcaattaccc tgataagctt tgcctggtcg taaggcctga
     3541 gctgatagtg aagccctctt ataacccctt tcgaagatct ggattcattg tcctgtatcg
     3601 gcatgaaatg tatacccgca tttctgtagg tatcctggtt ataactttca aagaaataac
     3661 cccttttgtc agaataaact gtcggcttaa tgacgaaaag atccttgaat ccggtatgaa
     3721 taatttccat tcagatcagt ttttctattt cattatgtcc ggtccggaga tttcttccga
     3781 ggctattctc atcaggtatt ttccgtactg gctgtttttt atgggttctg ccagggataa
     3841 cagctgattt ttgtcaatga atcctctttt ataggctatt tcctcgatgc atgatgcctt
     3901 caggctttgc ctgtgttcga gggtggctat gaagttcgag gcctgcagga gactctcaaa
     3961 tgttcctgta tcgagccatg ccattccccg gcccatgatc ctgaccgtaa gttttccttc
     4021 ttcaaggtat aacctgttga ggtcggttat ttcgagttct cccctgggag agggtttcag
     4081 tgaccgggcc ttgcttacca cagtattgtc ataaaaatac aggccggtga cagcatagtt
     4141 tgattttggc ctggccggtt tttcctcgat tgttacagcc cttccgctgc tgtcgaattc
     4201 cacaactcca taacgttcag ggtcggtaac ataatatccg aatacacagg ctccgtcatc
     4261 cagccttgcc gtattgcgga gcacgtcccc gaacccgtga ccgtaaaaaa tattatcacc
     4321 aagtatcatg cagaccggat catttcctat gaattcatct cccaggataa aggcctgggc
     4381 cagaccgtcg ggtgagggct gctcgatata gctgaattca aggcctattg attttccgtc
     4441 acccagaagg ttcctgaacg aaggcaggtc acggggtgtt gaaatgacca gtatttccct
     4501 gatacctgca agcatcagta ctgaaagcgg gtaataaatc atcggcttgt cataaacggg
     4561 taacaactgt ttcgatatcg tgttggttat cggatgcagt ctgcttcccg accctcctgc
     4621 cagaataatt cctttcatgt attttccctt ttgctgttta ttcagtgaat atactattta
     4681 atttcttcct gcggccttct tcctatgcca tagtatttaa atcccatttt tacgagcaaa
     4741 gccggatcgt aaatatttct tccgtcgaat atcaccctgt ctttcattat ccctgccatt
     4801 tttttaaaat tgggaagctg aaattccggc cattccgtca ttaatgccat tgcatcagta
     4861 ttttccagtg cttcataggg attatcacag tattgtattg aatctccgag gagctttttt
     4921 gtttccttca ttgcggccgg gtcatacgcc ctgacgactg cccccttatc aagaagcttt
     4981 cttatcaggt ggagggatga tgattcacgt acgtcatcag ttttgggctt gaaggataat
     5041 ccccatattc cgattgtctt tttttcaagc ctgccgctga agtgatactt catcttgttg
     5101 aaaatgatat ttttctgaaa ctcatttgct ttttcaactg ctttgacgac attgagttca
     5161 tagcccagtt cctctgccgt tttgattatg gccttcacat ccttgggaaa gcaggaacct
     5221 ccatagcccg gtccgggata gagaaacttg ctgcctattc ttgaatcact tcctattccc
     5281 cttcttacat ggtttatatc ggctccgaga aggtcacaga ggtttgccat ttcattgata
     5341 aagcttatcc gggtagccag cattgcattg gcagcatatt ttgtaatttc ggcagaaggg
     5401 atgtccatga atattatcgg gtggttatta agcacaaagg gtttgtagag acgctgcatc
     5461 acttcccctg cttccgggtt atccactccg accactattc tttcaggtct gagaaaatct
     5521 tcaagggcgg ctccttcctt aagaaattcc ggatttgatg ccatgctgaa tcggattccg
     5581 gactgtcttt tttcgagttg cctctgtatg gtttccctta ccttttcgga tgttcccacg
     5641 ggaactgtgc tttttgtaac gatcagggcg tagtctttaa gggaatcacc aatttcccgt
     5701 gcaacctcca gcacatgttt caaatccgca cttccgtctt ctcccggggg tgttcccacc
     5761 gcaatgaaaa taacctctga accctctatg ccttctgata ctgaggatga aaaaaacagc
     5821 cttccctttt ccacatttct gctgatcatt gtttccagtc ccggctcata gatggggatg
     5881 gtgcctttgt taagccccct gattttatcc gtatccctgt caatgcatgt caccgttact
     5941 ccggtctcgg caaagcaggt accggtaacc agtccgacgt atcctgtccc aattacactg
     6001 attttcatta aagctggttc gttgaggatt gaattaaaac atttgcaaag ataacagaag
     6061 ccctgagatt tgaaaacaga ttctgttaat tgcctaatcc cttcagtttg agaagccgga
     6121 accccggaaa caatgtttag ggcatagctg tttattccgg ccttgtatgc ccgggatgca
     6181 gatggtaaat tacttgtatt tgcatgaatt aatcctgtat ttgcaagaat taattgaaca
     6241 aaaaatctgt ctttttaaat aaatatccta tctttgcaca ccatttttca acaagataat
     6301 gtctaaccta ttaattatta gtatttttat taacaaatta tgacagatac caggaagaag
     6361 aacccgaaaa aagatgattt agctgacagt gagatcgtta aatccgccaa gaagctgaag
     6421 atcaaggaat caaaggataa attgcttgaa caggcctttg aagaagatat tattccggga
     6481 agccagtttg acattactga ttcagatgat gatccggtta aatctgaaac ggacgatctg
     6541 acagaattca ttcacggttc agaaagcgga ggggaagagg agaatgctgg tgattttcag
     6601 gaaaccgtgg aaacagtggc tgaatccggt gatttgacag ctgatgaatc tgatattcct
     6661 gagattgcag aaatttctgc cggcaaacct tcagttgagg attttgactg ggagagcttc
     6721 gagagcgagg aggcgaaact ttcacctaga cacaaggagt atgaggcaat gtatgataaa
     6781 accctttcaa cagttgcagt tgatgaggtt atctctggaa cagtaataca gatgacctca
     6841 agggaagttg tgatcaatat aggatataaa tcggaaggtg ttgtaagcct gaatgaattc
     6901 cgctacaatc ccggcctgaa ggtgggcgat gaggtggaag tgtatgttga aagtcaggaa
     6961 gataagaaag gtcagctcat tttgtcgcat aagaaggccc gggcgattaa atcatgggag
     7021 agggtgaatg cttctctgga aaatgatgaa ataattaccg gttacataaa gtgccgtacc
     7081 aaaggcggaa tgattgttga tgtattcggg attgaagcat ttcttccggg atcccagata
     7141 gatgtaaaac caatcaggga ctatgacata tatgtaggga agacgatgga attcaaggtt
     7201 gtaaagatta atcaggaatt caagaatgtt gttgtttccc acaaggccct cattgaagct
     7261 gagcttgaac agcagaagaa ggagattatt gcaaagcttg aaaagggcca ggttcttgaa
     7321 gggacagtca aaaatatcac atcttatggt gtattcatcg atctcggggg cgttgacggg
     7381 ctcattcaca ttaccgatct gtcctggggc agggttaatc atcccgaaga gatagttgaa
     7441 ctggatcaga aactgaatgt tgtcattctg gattttgatg atgacaagaa aaggatagcc
     7501 cttggcctga aacagcttac accgcatccg tgggattctg ttgatccgga tctcaaggtc
     7561 ggggacaggg tcacaggcaa ggttgtggta atggccgatt atggtgcatt tgttgaaata
     7621 gcacccggag tggaaggcct tatccatgtt tcggaaatgt catggtcaca gcatctgcgc
     7681 agtgcccagg aatttatgaa agtgggtgat gaggttgaaa ccgtgattct gactcttgac
     7741 agggcagaga gaaaaatgtc acttggcatc aagcagctga agccggatcc ctggcaggat
     7801 attgagcaaa aatatacagt cggttccagg cataccgcac gggtcagaaa ttttacaaac
     7861 tttggagtgt ttgtggaaat tgaagagggt gttgacggac tgatccatat ttctgatctg
     7921 agctggacca agaagattaa gcatcccgct gaatttactg ccattgatgc tgaaatagag
     7981 gttgttgtac ttgaaataga caaggaaaac cggaggctca gccttgggca caaacagctt
     8041 gaagagaatc cctgggatgt tttcgaagat ctgttcgcaa ccgattccat tcatgaaggt
     8101 actgtgatgg aagtgtttga caaaggcgct atcatagctc ttccctatgg agttgagggc
     8161 tttgcaaccc ctaagcatct ggtaaaggaa gacggcagcg ttgcgaaagt tgatgaaaga
     8221 cttatgttca aagttattga attcaataag tcggcaaaga aaatcattgt atcacacagc
     8281 agggtatatg aggacgagaa gaaggctgct gaagctcccg aaaagaagtc agaacccgaa
     8341 acaccaagga aatctgcaag gaaagccaag acgaatatcg aaagaaccac gctgggagat
     8401 attactcagc tggcggcact gaaggaagaa atggaagaaa aagagaataa agcttccaag
     8461 gaatagaaat atttgttatt tttgagccaa caaaacaata caatcatgga ttttaacatt
     8521 gaaagtcgta ataacagtac attgattcga attcagacgg agaagcttga tactcatata
     8581 gctcccgcat tgaagtctga acttgttttg atttcaggca agggtgaaaa aaatattatc
     8641 cttgatctgg aaaaaaccca atactgcgac tcttcaggtc tgagtgccat tcttgttgca
     8701 aacagactct gcaagaatgc cggcggtacc tttgtgcttt gcggtctcaa tgaagctgtt
     8761 gaaaggctca ttaccatttc ccagctggat acggtcctta caattaccga aacggttgct
     8821 gatgccgaaa aaatgatttc ctgaagctga caggaatgca gggtaccgat gaaattgaca
     8881 atacttggca gtagttccgc actgccaact tcagaaagat atccttccgc tcatgtactc
     8941 tccgtgcatg agcggttgta tttgatagac tgcggtgaag gtacccagat gcagctccgg
     9001 cgaaataaaa tcagattcgg aaaaataaac catatcttca tcagccata
//
LOCUS       DAOSPX010000103         9015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7353_length_9015_cov_3.526562, whole genome shotgun sequence.
ACCESSION   DAOSPX010000103 DAOSPX010000000
VERSION     DAOSPX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7353_length_9015_cov_3.526562"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1261)
                     /locus_tag="PLZ75_06610"
     CDS             complement(<1..>1261)
                     /locus_tag="PLZ75_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72107.1"
                     /translation="VTLRSVQYKATSRNKLALIALSENRLEEAWKYAMDAVEYNAKDR
                     NNYGTAAVVARLKGDRGSHEELLGKMLKMDPLNHFALFEKYYMLKDETSKTGFLSRIT
                     GEFKYETHIELALWYFNAGLLNEAISVMEICPENPVADYLTAYLMSLKNNAEKAQFYL
                     TRAINADDKLVFPYREEYAAVLEWADKQQQSWKTKYYSAILYWNRTRNDIAEKYFSEC
                     GDQPDSYSFYLSRGRFHDETGGRAETDYLKALKLGENNWRPYHTLYNYYVAHNKYDRA
                     LEITQKAINMFRDKYVIAFDHAMSLLYNGKYDESVRILERIRILPYEGAGYGRMAWKN
                     ANLLNALDYYSGRNYRRASVYAANAYKWPENLGVGRPYTVDERAEDFVNAMILERSGN
                     KRKSAELYENIARYNGGKPAGNRSMNYL"
     assembly_gap    1262..1271
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1272..2930)
                     /locus_tag="PLZ75_06615"
     CDS             complement(<1272..2930)
                     /locus_tag="PLZ75_06615"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOS72108.1"
                     /translation="MKKIIASSLLTLFLLAAYAQEARISEEYIPFTTYSFNDPDPVAR
                     PGSIFPYFRFDGFSYTPEVVKHKMIVLENKWIKVWVAPDIGGKVWGALDKKTNKYFIY
                     HNNVVKFRDIAMRGPWTSGGIEHNFGSIGHAPTTSTPVDYSWQKNDDGSVSCFVGSMD
                     LSSRTEWRVEVRLPKDKAWFETISHWYNPTDLKTSLYHWENASADGTNDLRFYFPGKA
                     EIGHGGEPGDWPVLKNGRDISLYGNNNFGSSKSYHVLGEYADWFAGLYERSNHGFGHW
                     SRQPVKPGKKIWIWSLGRDGEIWVDLLTDTQKGNTQYIEIQTGLLYNQAGDGSTRSPF
                     KHVFFEPGAVESFTELWFPLSDLAGVSTVSPEGALFAEKSENRCTIQFQALGYVRDKL
                     QITDTEGKVISEFELNLEPEQIFSRSVELENFNVKVRLANGELRYNLADREENFVDRP
                     LSMQENFDWESVYGLYTRGVEKMRQRQYSQAATLLEKCLEKDPFFMPALTALAETDIR
                     FMKFDEAERKLLEVIRFDTYDPDANFLYGTLLMRKKEFNKARDAF"
     gene            complement(3112..4893)
                     /locus_tag="PLZ75_06620"
     CDS             complement(3112..4893)
                     /locus_tag="PLZ75_06620"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOS72109.1"
                     /translation="MRNTESAHDVQTERTQDRLLAAALFSVSFLFFYFFRNTLFNYQE
                     STSLFIYTNEYLSKFTDKPGGLLVYLGNFLSQVYFSSFYGSIILALLNITLCFCIARA
                     GTALNADLILKRILLLSLFAVMLLFQSQYEHFIHYSLGFLMVAVCFSVIVTVGAGWRR
                     LIYFTGLPLFYYVAGPFAVLFVLMLISWSLVYEKGRFRFLNPLLMIVISIIAWFVFRN
                     YLFYQPARTLVLYPLPFTSFRQLHFSMIILALFFVFLPLFYKLTALVRITGRTRSCLF
                     PAFSLVLFLLLLFLSLKYHNSSLVKIIRLEGMIARQDWDGVIRQQEESPVANLIAQYY
                     YNLALSEKGLLCERMFRAPQDFGASSLCLPRHAEFYNRSVHFYYSAGLINEARHLAYE
                     SMVAYGYRPGNIDMLIKTELINGNFRVAEKYINDLKKTLHYRTKAIKYRKMLYRPEAV
                     KADRELGSKLLLLPGNDFFIRPDDRENINLLFLSNPLNLKAFEYRIAWMLLEKDYKSA
                     VHEIRRMKFLGYNIIPRHLEEAVVGYANISGRMPDLGGLSLRPETERDFFEYGSAYNL
                     FSRDKETLAGEMKKTAGKTYWYYLQFK"
     gene            complement(4880..6352)
                     /locus_tag="PLZ75_06625"
     CDS             complement(4880..6352)
                     /locus_tag="PLZ75_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72110.1"
                     /translation="MNCGRGSHVEAAVNMERLALTDPDYSETIIPWNIAPMNFSILEE
                     YKTCKIRLSGTDGSSLTVNTSGKTVRFPRKKWKKLLLRNRAGRISVEIMAENGREKAM
                     KFKPFNFYIADEHTDPYLVYRILYPGYEAWLDMKIVQRSTENFSEKSIVENQLLDNNC
                     VNCHSFANNDPSKMLLHVRGTKSGTYISENDKIVRRSLKTDNMPANTVYPAWHPSGNY
                     IVFSSNKTVQAFHSRPEKNIEVFDLFSSLVIYDVKRNEIMNCMQDDSVEYMETFPCWS
                     PDGTWLYYCRTPQVREGFDFREVKYDLVRIPFSQQSGNFGKPEMLFNAAAAGKSVSFP
                     AVSPDGRYVVFTLHDYGTFSIWHREADLYILDISGGRISKMPINSNESESYHSWSSSG
                     RWLVFSSKRDDGLAARPYLCYFHSADSIGKPFILPQKDPQVYTRMKKTFNRPEFVTGK
                     IETGPRDFEKASRSEPVQAVWTGSSELKSHKDERYGNEKY"
     gene            6687..>9015
                     /locus_tag="PLZ75_06630"
     CDS             6687..>9015
                     /locus_tag="PLZ75_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS72111.1"
                     /translation="MRKFTLFIAAFFFIGLQSVFAQKLVSGTVKSAEDGLGIIGATVV
                     VPGTTIGTTTDASGAFTLNVPTDAQSLSFSYVGMKTVELSIGTQTVFNVTLEPDVMAL
                     QDVIVTAFGISRQTKSLTYAAQSVTTDALTEARNPNVMSGLSGKVSGMLITQTGQGVG
                     GSTKVLLRGNRSISGSSQPIYVVDGITLNGGIENISPDEIESISVLKGANAAALYGSR
                     ANNGAIVVTTKSGKGARQGVTTSLGFTYQANQAMILQKVQNIYGQGANGMYAKAATVA
                     WGPKMEGQMVDHWSNDPSYYMYGKQYPFLPQPNNIKDFFRIGNSFSTNLGVNINSDVS
                     NITFSYTNSNDGGIIDGNDLKGHNLNLRVRANLSRKLTLDSKLNVIKRNYSNVFITGE
                     TFENPMRYLYMLPRNIRSEDIQHYEFINPAGQLRQHYWKVNDNGSGNPYWSVYNAVQP
                     SKSQRAIAMMSLKYEFLPGLSIQGRSGFDGTYTNTEWKRNNDTYTNAYYGAFSKTSSN
                     SYEWNNDFLINYNKNFGEFALDLNAGGNHRLYESENVSGSGSIFNIENMFALANTKDP
                     RPGEGYSKKVVQSFYGFGELSWRNALFLNITGRNDWSSTLPAASRSYFYPSVGLTAVL
                     SDLMTLPAAFTHFKLRTSYAEVGNDAGAYNLHRSASVSLGTIGLSTSLPNPNLKPERT
                     RSIEAGLDLRMFNDRGRLSGTWYQTNTYDQLFSTPVPVTSGVSSVYQNGADVQNRGVE
                     LTLGAVVVDKRDFSWDIMLNWSKNVSKILEIAEGFD"
BASE COUNT         2481 a         2098 c         1946 g         2480 t
ORIGIN      
        1 ataaatagtt catagatctg ttcccggcag gttttccgcc attgtaacgt gcaatatttt
       61 catacagttc cgctgatttc cttttgtttc ccgacctctc cagaatcatt gcatttacaa
      121 aatcctcggc cctttcatcg actgtatacg gtctgccaac cccaaggttc tcaggccatt
      181 tatatgcatt agctgcataa acggatgctc tcctgtagtt tctgcccgag taatagtcca
      241 aagcattcag caggtttgca ttcttccagg ccattctccc atatcccgca ccttcatacg
      301 gaaggattct gattctttca agaatcctga cactttcgtc atattttccg ttgtagagca
      361 gggacatggc atgatcaaaa gctattacat atttgtcccg gaacatgttg atggctttct
      421 gggtaatctc cagcgccctg tcatatttgt tgtgcgcaac ataatagttg taaagggtat
      481 gatagggcct ccagttattt tcacccagtt tcagtgcctt caggtaatcc gtttcggccc
      541 gtcctcctgt ttcatcatga aatctgcccc tgctcagata aaaggaataa ctgtcgggct
      601 gatcaccgca ttcactgaaa tatttttccg cgatgtcatt acgtgttctg ttccagtaga
      661 gaattgcgga ataatatttg gttttccagc tctgctgctg tttgtctgcc cattcaagaa
      721 cagcggcata ttcttccctg tagggaaaaa ccagtttatc atcggcatta attgcccttg
      781 taagataaaa ctgtgccttt tcggcattat ttttcagtga catcagataa gcagtcagat
      841 aatcggctac gggattttcc ggacagattt ccataacgga aatagcttca ttcagaaggc
      901 cggcattaaa ataccagaga gcaagttcaa tatgggtttc atatttgaat tccccggtaa
      961 tcctcgataa aaagccggtt tttgatgtct catccttcag catataatat ttctcgaaca
     1021 aggcaaaatg attgagagga tccattttca gcattttccc aagaagctcc tcatgtgatc
     1081 ccctgtctcc cttcagcctt gccacaacag ctgccgtacc gtagttgttc ctgtccttgg
     1141 cgttatattc cacggcatcc attgcgtatt tccatgcttc ttcaagcctg ttttcactta
     1201 aggctatcaa tgccagcttg ttacgggaag tcgccttata ctgcactgac ctgagcgtga
     1261 cnnnnnnnnn ngaaagcgtc acgggccttg ttgaattcct ttttcctcat aagaagtgtg
     1321 ccgtagagga aattggcatc aggatcatag gtatcaaaac ggattacttc gagcagtttc
     1381 cgttcggcct catcaaattt catgaaacgg atatctgttt cagccagtgc tgtcagtgcg
     1441 ggcataaaga acggatcctt ttccaggcat ttttcaagca gggtggcagc ctgtgaatac
     1501 tgacgctgtc tcatcttttc gactcccctg gtatacaagc cataaactga ttcccagtcg
     1561 aagttttcct gcatgctgag gggacgatca acaaagtttt cttctctgtc agcaaggtta
     1621 tacctgagtt caccgtttgc caggcggact tttacgttaa aattctccag ttcaaccgac
     1681 ctggagaaaa tctgttccgg ttccagatta agttcaaatt cggagattac cttaccctct
     1741 gtgtcggtta tctgcagctt atccctgaca tagccaaggg cctggaactg gattgtgcac
     1801 ctgttttcgg atttctccgc aaaaagtgct ccttcggggg aaacagtgct tactcctgcc
     1861 aggtcgctca ggggaaacca taactctgta aagctttcga ctgcccccgg ttcaaagaac
     1921 acatgcttga aagggctcct ggtactgccg tcccctgcct gattataaag cagtccggtt
     1981 tgtatttcaa tatactgggt gttacctttc tgtgtatcgg tcagaaggtc aacccatatt
     2041 tcaccgtccc ttccaagtga ccatatccat attttctttc cgggcttcac cggctgcctt
     2101 gaccagtgtc cgaatccatg gttgctccgt tcatacaatc cggcaaacca gtcggcatat
     2161 tccccaagca cgtgataact ttttgatgat ccgaaattgt tattcccgta cagggatata
     2221 tcccgcccgt ttttcagcac gggccagtca cccggttcgc cgccatgccc tatttccgcc
     2281 ttgccgggaa aataaaacct cagatcattc gtgccgtcag ccgaggcatt ttcccagtga
     2341 tagagggaag ttttcaggtc tgtcggatta taccagtggg atattgtttc aaaccatgcc
     2401 ttgtctttcg gcagccttac ctccaccctc cattctgtcc gtgaggacag atccattgaa
     2461 ccgacaaagc acgaaacact tccatcatca tttttctgcc aggaatagtc aacgggcgtc
     2521 gatgtggtgg gggcatgacc aatcgatccg aaattatgtt ctataccgcc tgaggtccac
     2581 ggtcctctca ttgcaatatc cctgaatttc actacattat tgtgataaat gaaatacttg
     2641 ttggtttttt tgtccagggc accccatacc ttgcccccta tatccggagc aacccacacc
     2701 tttatccatt tgttttcaag tactatcatt ttatgtttta ccacttccgg agtatatgaa
     2761 aatccgtcaa acctgaagta cggaaaaata gatccggggc gtgcaaccgg atccggatca
     2821 ttaaaggaat aggtggtaaa tggtatgtat tcctcactga tcctggcttc ctgagcatag
     2881 gcagccagca ggaacaatgt taacaagctt gatgcgatta tttttttcat aatgttattt
     2941 ggttttaaat tagccggttg ataatcttcg ccgttaccgg gctgctgaaa cataccgggc
     3001 cgttgttgtg aaatgttgtg actgaaagat gaagtaataa cattctgcaa tattataatt
     3061 cctgacgata ataaaaatat ttggcaggcc tgaaacagaa atttttaatg tttatttgaa
     3121 ctgaaggtaa taccagtaag tttttcctgc tgttttcttc atttccccgg ccagggtctc
     3181 tttatccctg ctgaaaaggt tatatgccga gccgtattcg aagaaatccc tttccgtctc
     3241 aggtctcagg ctcagacctc ccagatcagg catccggccg ctgatgtttg catagcccac
     3301 cactgcctct tccagatgtc tgggaataat attgtaaccc aggaatttca ttctcctgat
     3361 ttcatgtact gcagatttat aatccttttc aagcagcatc catgcgattc tatactcaaa
     3421 agcctttagg ttcaatgggt tcgacagaaa gagcagattg atgttttcac ggtcatcagg
     3481 tctgataaaa aaatcgttcc ccggcagcaa cagaagctta ctccccagct ccctgtcggc
     3541 ctttacggct tccggtctgt agagcatctt cctgtattta atggcttttg tccggtaatg
     3601 aagagttttt ttcagatcat tgatatattt ttctgctacc ctgaaatttc cgttaataag
     3661 ttcggtttta ataagcatgt caatgtttcc cgggcggtag ccatatgcta ccattgactc
     3721 gtaggccagg tggcgggctt cattgataag tccggccgaa taatagaaat ggaccgaccg
     3781 gttataaaat tcagcatgcc ggggaaggca gagtgaggat gcaccgaaat cctgcggcgc
     3841 tctgaacatt ctctcacaga gcaatccttt ttctgataat gccaggttgt aataatactg
     3901 ggctatcaga tttgcaacgg gcgactcttc ctgctgcctt atcactccgt cccagtcctg
     3961 ccgtgcaatc attccttcca ggcgtattat tttcacaagg ctggaattgt ggtatttcag
     4021 ggataaaaag agcagaagca gaaaaagcac cagtgaaaag gccggaaaaa gacagctccg
     4081 ggttctgcca gtaatcctga caagagcagt cagtttataa aaaagcggaa ggaaaacaaa
     4141 aaacaatgcc agaattatca tggagaaatg caattgcctg aaagaagtga agggcaatgg
     4201 ataaagaaca agcgtccggg ccggctggta aaaaagataa ttccggaaaa caaaccaggc
     4261 aataatgctg ataactatca tgagaagagg attaaggaag cggaaacgac ccttctcata
     4321 aacaagggac cagctgatca gcattaagac aaacaaaact gcaaagggtc ctgcaacata
     4381 gtaaaacagt ggaagtcccg taaaataaat taatcttcgc catcctgcac ctactgttac
     4441 aatcactgaa aaacagactg ccaccatcag aaagcccagg ctgtaatgaa tgaaatgttc
     4501 atactgcgac tggaataaca gcataacagc aaaaagggac agtaacaata tccttttaag
     4561 gatcaaatct gcattcaggg cagttccggc cctggcaata cagaaacaga gggtaatatt
     4621 aagcagtgca aggattatcg aaccgtaaaa agatgagaaa taaacctgac tgagaaaatt
     4681 tccgagatat acaagcagtc ccccgggttt gtctgtaaat tttgagagat actcattcgt
     4741 atatataaac aatgatgtgc tttcctgata gttgaaaagg gtatttctga aaaagtaaaa
     4801 aaacaggaat gacacagaaa aaagggctgc agccagaagc cggtcctgtg tccgttcagt
     4861 ctgaacatca tgggcgctct cagtatttct cattcccgta cctttcatcc ttatggcttt
     4921 tcagttctga agatcccgtc caaacggcct gaaccggctc tgaacgggaa gccttttcaa
     4981 aatcacgggg tccggtctca atttttcctg taacaaattc cggtctgttg aatgtttttt
     5041 tcatacgggt gtaaacctgc ggatctttct gcggaagtat gaatggtttt ccaatgctgt
     5101 cggccgaatg gaaataacat aaatatggcc gtgccgcaag tccgtcgtcc ctcttgctgc
     5161 tgaagaccag ccatctgccg cttgaagacc atgaatgata actttcagac tcattgctgt
     5221 tgatcggcat ttttgaaatc ctgccgccag aaatgtcaag aatatacaaa tccgcctccc
     5281 tgtgccatat tgaaaaagta ccgtaatcat gaagggtaaa tacaacatat ctgccgtcag
     5341 gtgaaacggc tggaaaggaa acgcttttac ctgctgcagc cgcattgaac agcatttcag
     5401 gcttaccgaa attccctgat tgctggctga agggaattct caccagatca tatttaactt
     5461 ccctgaaatc aaatccttcc cttacctgcg gggttctgca gtaataaagc catgttccgt
     5521 ccggtgacca gcaggggaaa gtctccatgt attcaacaga atcatcctgc atgcaattca
     5581 ttatttcatt ccttttaaca tcatagatga caagggacga gaacaggtca aatacttcga
     5641 tattcttttc cggtcttgaa tgaaaggcct gaactgtttt attcgatgaa aacacaatat
     5701 aattacctga aggatgccag gcaggataaa ccgtattggc cggcatattg tcagttttca
     5761 gggagcgtct cacgattttg tcattttccg aaatgtatgt tcctgatttt gtaccccgta
     5821 catgcaaaag catcttagag ggatcattat tggcaaagga atggcagtta acgcaattat
     5881 tatccagaag ctggttttcc acaatggatt tctcactgaa gttttcagtt gatctctgaa
     5941 ctattttcat gtcaagccat gcctcatatc ccggataaag aatcctgtaa accaggtaag
     6001 gatctgtatg ctcatccgca atatagaaat taaaaggctt gaacttcata gctttctccc
     6061 ttccattctc cgccattatc tccacggaaa ttctgcctgc cctgttccgg agaagcagtt
     6121 ttttccattt tttccggggg aatcttaccg tttttcctga ggtattcact gtcagtgaac
     6181 tgccgtccgt tccgcttaac ctgattttac aggttttgta ttcctccagg atgctgaagt
     6241 tcatgggggc aatattccag ggtattattg tttcggagta atcaggatct gtcagcgcaa
     6301 ggcgttccat gtttactgct gcttcaacat gactgcctct gccacagttc atgagaagaa
     6361 ccggcaataa tgtgaccaac agaaatcctg gaaatatcat tctgctgatt cgcaaccgtt
     6421 tttcggaaaa tttcattgct gtttcttctg ttttagggtc agaccaggcc ttttgcgcag
     6481 tatctccgga aagatttcaa atgtattgaa attttcagaa aatacaaaaa atgaaaaaaa
     6541 caacaaaaac actatgatat ttgaaatatt taacataatt taacgcacga attaccaaat
     6601 atgcttaaca gtaatattta aaaccggatt tgagaattat ttattcaatt ttcattttta
     6661 tctaaaacta acttaatgtt ctaactatga gaaaattcac tttgtttatt gcagccttct
     6721 tttttatagg cttgcagagt gtatttgcac agaagcttgt ctccggtacc gtaaaaagtg
     6781 ccgaggacgg gcttggcatt atcggggcaa ctgtggtggt gccgggcacc acgataggta
     6841 ccacgactga tgcaagcggg gcatttaccc ttaatgttcc aaccgatgca caaagcctgt
     6901 ccttctccta tgtgggcatg aaaacggttg aactgtcaat aggaacacag actgtgttca
     6961 atgtaacact ggaaccggat gtaatggcac tgcaggatgt tattgtcacg gcattcggta
     7021 tttcgaggca gacaaaatca cttacctatg cagcccagag cgtaaccacc gatgctctta
     7081 ccgaggcaag aaatcccaac gtaatgtcgg gactaagcgg aaaggtgtcc ggaatgctta
     7141 ttacacagac cggtcaggga gtggggggaa gcaccaaggt tctgctcagg ggaaaccgct
     7201 ccatcagcgg aagcagtcag ccgatttacg tggttgacgg tattacgctc aacggaggaa
     7261 ttgaaaacat cagtcccgat gaaattgaat ccatttcggt tctgaaggga gcaaacgccg
     7321 cagctcttta cggaagccgt gcaaacaacg gggcaattgt tgtaacaaca aaatcaggga
     7381 agggtgcccg tcagggtgtc actaccagtc tcggctttac ctaccaggca aatcaggcca
     7441 tgatcctgca gaaggttcag aatatctacg ggcagggagc aaacggtatg tatgcaaaag
     7501 cagcgacagt ggcatggggc ccgaagatgg aagggcagat ggtcgaccac tggtcaaacg
     7561 acccctccta ctatatgtat ggtaaacaat atccgttttt gcctcagccc aacaacataa
     7621 aggatttttt caggatcggc aacagtttct caacaaacct cggggtgaac atcaattctg
     7681 acgtgtcgaa tataaccttc tcatatacca acagcaacga tggagggatt attgacggta
     7741 atgacctgaa aggacataac ctcaacctga gggtacgtgc caacctttcc aggaagctta
     7801 cactggattc aaaacttaat gtaattaaaa ggaactattc caatgtgttt attaccggtg
     7861 aaacatttga gaatcccatg agatatctct atatgttgcc caggaacata cgttcggaag
     7921 atattcagca ttatgaattc attaaccctg cgggtcagct gcgccagcac tactggaaag
     7981 tgaacgacaa cggttcggga aacccttact ggtccgttta caatgcagta cagccatcaa
     8041 agagccaaag ggcaattgca atgatgagcc tgaaatatga attcttgccc ggactctcaa
     8101 ttcagggcag gtcagggttt gacggcacat atacaaacac ggaatggaag cgcaacaacg
     8161 acacctatac caatgcctat tacggagctt tttcaaaaac cagttccaat tcctatgaat
     8221 ggaacaatga cttcctcatc aactacaata agaattttgg cgaatttgca ctggacctca
     8281 acgccggagg caaccacagg ttgtatgaat cagaaaacgt aagcggttcg ggttcaattt
     8341 tcaatattga aaacatgttc gcccttgcca acacgaaaga tccgaggccc ggcgagggat
     8401 attcaaagaa agtggttcag tccttttacg gattcggtga attatcctgg agaaatgcct
     8461 tgttcctgaa tattaccgga cggaatgact ggagctccac cctgccggct gcaagcagat
     8521 catattttta tccctctgta gggctgacgg ctgtactgag cgatctgatg accctgcctg
     8581 ctgcatttac tcacttcaag ctcaggacat cctatgccga ggtgggaaat gatgcaggag
     8641 catacaatct ccacaggtca gcttcagtat ccctgggcac aataggactc agcacatcac
     8701 ttcccaaccc gaacctcaag cccgaaagga caagatccat tgaggccggg cttgatctca
     8761 gaatgttcaa cgacaggggc aggctgagcg gaacatggta tcagacaaat acctatgatc
     8821 agctgttctc cactccggtt cccgtgactt cgggagtgag cagcgtttat cagaatggtg
     8881 ccgatgtcca gaacagggga gtggaactga ccctcggagc agtggtggtt gacaagcgcg
     8941 atttctcctg ggacatcatg ctcaactggt cgaagaatgt cagcaaaatt cttgaaattg
     9001 cagaaggatt cgacg
//
LOCUS       DAOSPX010000104         8998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7370_length_8998_cov_4.801409, whole genome shotgun sequence.
ACCESSION   DAOSPX010000104 DAOSPX010000000
VERSION     DAOSPX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8998
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7370_length_8998_cov_4.801409"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..228
                     /locus_tag="PLZ75_06635"
     CDS             34..228
                     /locus_tag="PLZ75_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72112.1"
                     /translation="MKDPAQKISGKEVYTRPEIHRVILDNSISLVMMTDIPPNPPPRE
                     NYRKGNDSPFESPFGDKPFA"
     gene            462..2834
                     /locus_tag="PLZ75_06640"
     CDS             462..2834
                     /locus_tag="PLZ75_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS72113.1"
                     /translation="MKQRVAFLSVLFLSVSLLFGQPQETVYEGTLIATGLRQSIPIPS
                     SGPFDIGFNFTFFGNTYNHFYVSANGLVMFTQPDGLYNDEVTIPDPAMPNNYIAPFWD
                     NLSILDRGNVMYRTIGTAPGRKCIIQYKNMGFDPVLKPFGTFSVILYETTNVVQIQYR
                     LIVDPFSPEPHGASATIGLENADGTAGVLYAFHEDDAVYTGDAISFTPAGPDDYTIND
                     NAIYDGVFLTSNLTLPDPERVDLVSPAKDAITGTNHTFIWTASSKATNYYLLLDSKPD
                     LSDASYTSVGLNLNHTVTGLEPDKTYYWTVFAENSTGIAWCEVSRFTTSTTPPLSAVP
                     QTIWMEQGQEKTIQLNYTGGDGSTKTAVITSLPAQGILYQYNAGGRGAQITSVPATVT
                     DAGRNIIYSASGNAGNNAGNFRFIMSDNTGNSPEATIKINVSPPGVPSVLYMAKNINV
                     EIQFDRLMADPAGKQNQFEVKVNGLPAAVSSVSLKTGDPYTIVLNLSVPLTGTENVTL
                     SYNQGDVASTSGGLLLPFTGQPVTLKAQTITFTQSLDRKYSLQSFTLSATASSSLGIT
                     FASANPAVAVISGNRTCNFRGVGTSSITARQAGNATYAPARYIRTLTVSKGDQTITFD
                     PIPAQALEYPDFNLVATATSGLPVSFSSSDPGVATVTGSQVHITGTGTTVITAAQAGS
                     ELWNPAADVQQPLVVNATGIDDAFAPENSFNIYSAGNYIYVRTNAGEQDGMTGTVTIF
                     DITGKPVSILENIEFSGDSRIEIPAGDMKGLYIVEIRTATIKARKKVVVR"
     gene            3315..3698
                     /locus_tag="PLZ75_06645"
     CDS             3315..3698
                     /locus_tag="PLZ75_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72114.1"
                     /translation="MNNQTIGTRFFHRFGLRSKVVIDFFNIKEERDGGNRQEYPLHER
                     DVAEAVKHNIKTGAVTYEHYFRDYDLFTLFASTQSLKRDSYYGANHSFADYGYFNLDW
                     DFAKDIYFSVTGTYSGKSLGTPAEI"
     gene            complement(3753..4580)
                     /locus_tag="PLZ75_06650"
     CDS             complement(3753..4580)
                     /locus_tag="PLZ75_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72115.1"
                     /translation="MDLFFNEISAYYPAKSKFDAQQKMSAFLELCKKAKQEGFNSCKV
                     VNNFEETELAATYTIHNWLGDTEINKNQKDFYFSFRNSPYEKGIEHTFDAFCYLDEPT
                     EPNYNGSIVEGLMWANLCDSFALSFPVNEVWRKSLINIEFDDKPDRRKDNVKHISSLL
                     HFDYYQEFIKSQKEPVLVPSDINPSEKRIHLSDDHGKDKLYALAKKLVNCDYVIEIPH
                     SLPFDRHGRSFIREIHPNGRIDVTLIEEDDGYSMMVQTTGKTLWETQEIAKILKELY"
     gene            complement(4583..5713)
                     /locus_tag="PLZ75_06655"
     CDS             complement(4583..5713)
                     /locus_tag="PLZ75_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HOS72116.1"
                     /translation="MIKYIAIKNFKAFKSLKLKPSNLNLCFGMNGMGKSSLLQSLLLL
                     RQSFQKGLLDTKGLLLNDKDLISIGTGKDAYYQSAGRGEHIEFEIQTQKGQRYQWAFQ
                     FDPTTDILPIEHGGNLIINQISKFEGFSLFNNHCQYLSAEHYGPQKLYPKSELEVKQN
                     KNIGIKGEYAVHYLSMFGNSEIIQLENLRHLKASSNNLLHQTAAWLGEISPGTKLVIE
                     DLKGVDSLRLGIQFETKTRFTNEFSPYNVGFGILYVLPLIVSLLKAEPGSLLLIENPE
                     SHLHPTGQSAMGRLMALAAENGVQIFCESHSDHIINGIRVAVKDNVLDHDKLAIYYFD
                     RNPNDDEHRSRVTEIFIDKKGTLSEYPAGLLDEWSNLLLKLI"
     gene            complement(5706..6842)
                     /locus_tag="PLZ75_06660"
     CDS             complement(5706..6842)
                     /locus_tag="PLZ75_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOS72117.1"
                     /translation="MVITKNYDNAKDLVEDELLYYGPVAEISKPFDPKKIDIQTKQMI
                     LEAIFRRLRNGEINMLTGFQRKSDLWDETKQSRLIESILIRIPLPAFYFDGTNDNDWL
                     VVDGLQRLSTFKNFVINKILTLQNLEYLTQFNGFKFDNLPRDLQRRIEEHEITVYIIN
                     PGTPPEVKYNIFKRINTGGLILEPAEIRHALNQGKAADYIKKLAEIQEFTSATTNSIP
                     TERMLDRDFVTRFIAFYLHPVSEYKGNLEEYLNSAVSELKDLSEARLQTITNDFISSM
                     KAARAIFGNRAFRKIYKKSDKRKPLNKALFEVWSVLLAKLSDTERNKLITNKDFVIEQ
                     FMESLNSSDKKLDKAISTGTADKQNVINRFSIIETVIRNSLAHD"
     gene            complement(7071..8093)
                     /locus_tag="PLZ75_06665"
     CDS             complement(7071..8093)
                     /locus_tag="PLZ75_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72118.1"
                     /translation="MEKKISLTNTKSEILNAYNELLKRIQDSKQESPKAEQEKKVKEA
                     TVEDAAKLTDEEIIGAISSLKLSLNSKLDRIEDDLTTERQKLAKIREAILIEDQRLKD
                     LYQINAGADSLAVILAVQKEKKEEFERETTQRKSELEEEIRQEKLIRDKETKLWEENR
                     KEAEETLKKQRVREEEEFQYNLKLTRKKDKDEYEQKKFQLEKELKEKLEAFDSGIKLR
                     EQSVAAAEQELEGLRSKAESFPAELEKAVQVAVKDVTSRLEKDYKFEKQLLLKDHEGE
                     VKLKNQQIESLIAKINDLNIQLKQAYSKAENAENNSKEITLKAIQASGQIKIIEKEET
                     RRKVEE"
     gene            8415..>8998
                     /locus_tag="PLZ75_06670"
     CDS             8415..>8998
                     /locus_tag="PLZ75_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72119.1"
                     /translation="MKIKSGYIIIFIFIYLFLPVERISSQEIPHPVANTGIYQFLDEL
                     ANDQVVQLSSVVKPYSRLFIARCLRDADEKRDRLSPRQQRELDFYLRDFGKEDSSRNK
                     LFRSSFSSNFEKVSSAREDLFSRLIKVIRGNKRWDLFYYSDSLFSLTINPIIGGEFFT
                     NSSGTATYWRNGIEARAYVNRWGFYASLRDNHEKP"
BASE COUNT         2560 a         1939 c         1609 g         2890 t
ORIGIN      
        1 attttgtaac aattctgtaa tacatctctg cttgtgaaag atcctgcaca aaaaatatca
       61 ggaaaagaag tatacaccag gccggaaata cacagggtaa tccttgacaa ttcgatctcc
      121 ctggtgatga tgaccgatat tcctcccaac cctccgccaa gggaaaacta cagaaaggga
      181 aacgattccc cctttgaaag tccgttcggg gataagcctt tcgcttagat tattcattca
      241 ttcaccatag tattgaatgt ctttcatcaa tttaagtcat aataatcaac acttttcgta
      301 aattcttatt acttttatac attgattaat attaatcctg gtataaaact gttgctgatt
      361 cctgttgtat tgattttaaa ttttccggat aaaggaagcg tgtgcaacta atttattgcc
      421 cggattgtcc tataatttga ataacagtta attcaccgga tatgaaacag agggtagcat
      481 ttctttcagt tttatttctt tcagtttcgt tgctgtttgg ccagcctcag gaaacagtgt
      541 acgaaggcac acttatagcc acgggtttgc ggcagagtat acccattccc tcgtcaggcc
      601 cctttgacat tggctttaac tttacctttt tcggcaacac ctataatcat ttctacgtga
      661 gtgccaacgg acttgtgatg ttcacacagc cggacggact ctacaatgac gaggttacaa
      721 tccccgaccc ggcaatgcca aacaattata ttgctccgtt ctgggataat ctttccattc
      781 tggaccgggg aaatgtaatg tacaggacaa tcggcacagc tcccggcagg aaatgcatca
      841 tccagtacaa aaatatgggc ttcgatcctg ttctgaaacc tttcggaaca ttctcagtga
      901 tcctatatga aactacaaat gttgtccaga tccagtacag gctgatagtt gacccattct
      961 ctcccgaacc ccatggggcc agtgccacaa tagggcttga aaacgctgat ggaacagcag
     1021 gcgttttata tgcattccat gaggatgacg ccgtctatac aggagacgcc atctccttca
     1081 ctcctgccgg tccggatgac tataccatta atgacaatgc catctacgac ggagtgtttc
     1141 tgaccagcaa tctcacactt cctgatcccg aaagagttga tctcgttagt cctgcaaaag
     1201 atgcgataac gggtacaaac catactttta tctggactgc ctcctccaag gccacaaatt
     1261 actatctgct cctggacagc aaaccggatc tgtcagatgc ctcctatacc agtgtcgggc
     1321 ttaatctgaa ccatactgtc accggcctgg agcctgataa aacatattac tggactgttt
     1381 ttgccgagaa ttcaaccggc attgcctggt gcgaagtaag ccgtttcacc accagtacaa
     1441 cgccaccgtt atccgcagtg ccacagacca tctggatgga acagggccag gagaaaacaa
     1501 tacagcttaa ttataccggg ggagatggaa gcacaaaaac tgcagtcatt acttctctgc
     1561 cggcccaggg catactctac cagtataatg caggaggcag gggagctcag attacttccg
     1621 tgcctgcaac agttactgat gccggcagga atattattta ttccgcttca ggcaatgccg
     1681 gtaacaatgc cggtaacttc aggtttatta tgagtgataa tacaggcaac tcccccgagg
     1741 caacgataaa gatcaatgtc agtcctccgg gagtcccttc tgtgctttat atggcaaaaa
     1801 atataaatgt ggagatacag tttgacaggc tgatggctga tcccgccggg aaacagaatc
     1861 agtttgaagt gaaagttaac gggttaccgg ctgctgtcag cagcgtgagc cttaaaacag
     1921 gggatcccta tacgattgta ttaaatctct ccgtccccct gacgggaacc gaaaatgtaa
     1981 cgctctcata caaccagggg gatgtggcat caacaagcgg cggactgctt ctgccattca
     2041 ccggtcagcc cgttactctt aaggcacaga ccatcacttt cacacaatcc cttgacagga
     2101 aatacagcct gcagtcattt acactcagtg caacagcttc cagcagcctt ggaataacct
     2161 tcgcaagtgc aaatccggca gttgctgtca tcagtggcaa cagaacctgt aacttcaggg
     2221 gagtgggaac atcttccatt acagcccggc aggcaggaaa tgccacgtat gctcctgccc
     2281 gttatatcag aacactgaca gtcagcaagg gtgatcagac tatcacattc gatcccatac
     2341 ctgcccaggc actggaatat cctgatttca accttgttgc aacggcaacc tcaggactcc
     2401 cggtcagttt ctccagcagt gaccccggtg ttgcaacagt aacaggatct caagtacata
     2461 ttacaggtac aggaaccacg gttatcacag cagcacaggc cggttcggaa ttatggaatc
     2521 cggctgccga tgttcagcag ccacttgttg tcaatgccac cgggatcgat gatgcttttg
     2581 ctccggaaaa cagttttaat atctattcag ccggtaatta tatctatgtc cggacaaatg
     2641 caggagaaca ggacggaatg accggtacag taacaatttt tgatatcacg ggcaaaccgg
     2701 taagcatact tgaaaatatt gaattctccg gagatagcag gattgaaatt cctgccggcg
     2761 atatgaaagg actgtatata gttgaaatcc gtacagccac aataaaagca agaaagaaag
     2821 tggtggtaag ataaaaccgg acttaacaat tttaaacgtg tgttttttca attacacctg
     2881 ttaatcattt aacagggccg ggaaattcta taagaaaata caaaaatatt gcaggactct
     2941 tttttgtttt actctatctc ggatatctcg gatcagtgac ctttttccac cacagccatt
     3001 ctgtcggcga tataacagtt gttcattccc atccgcacaa atcccataat gataattctc
     3061 attccggtca cgatcattca gaatatctgc ttgtactgat ccatttcctt tcggtaatca
     3121 taaccgccgg cactgtcttt ttttacggga taacaattac gcaaaaactt tattacagga
     3181 tcttttcatg ccggaacgag atcagcatcc cgaattttaa tttctcaggg ataaaccgtc
     3241 cctgggcacc ttcactccgc taaaccggcc cagacatcat aatattattg aatgtaagat
     3301 ccgaaatagc cccgatgaac aatcagacaa taggaaccag gttttttcac agatttggtc
     3361 tgagaagtaa agtagtcatc gattttttta atataaagga agaaagagac ggaggcaaca
     3421 gacaggaata tcccttacac gaaagagatg ttgcggaagc tgtaaagcac aacatcaaaa
     3481 ccggtgcagt gacttatgag cactacttca gggactacga cctctttacc ttattcgcat
     3541 caacccagag cctgaagagg gattcatact acggggcaaa ccactcgttc gccgattatg
     3601 gctatttcaa tctcgactgg gattttgcca aagacattta tttctctgtg accggaacct
     3661 attcaggaaa atctcttggc actcctgcag aaatctaaaa attttatgcc ccatgatatc
     3721 tgtcacttac atctcccaac ctgcaatgtg gactaatata attctttcaa tattttagca
     3781 atctcctgtg tttcccataa agttttacca gtagtttgta ccatcatact atatccgtcg
     3841 tcttcttcaa tcagggtaac atcaatcctg ccgttgggat gtatttcacg aataaagctc
     3901 ctgccatgtc tgtcaaatgg caaagaatgc ggaatttcta taacataatc acaattaaca
     3961 agctttttag ccaatgcata aagcttgtcc ttgccatggt catcgcttaa atgtattctt
     4021 ttttctgatg gattgatatc agagggtact aaaacaggtt ctttttgtga tttaataaat
     4081 tcttgataat aatcgaaatg taaaagtgaa ctaatgtgtt ttacattgtc ctttcttcta
     4141 tctggtttat catcaaattc aatattaata agactttttc tccatacctc gttaacaggg
     4201 aaacttaatg caaaagaatc gcaaagatta gcccacatta aaccttcaac aatgcttcca
     4261 ttgtaattgg gttcggtagg ttcatccaaa taacaaaatg catcgaatgt atgttcaatt
     4321 cctttttcat acggcgaatt tcgaaaactg aaataaaagt ctttttggtt tttatttatc
     4381 tcagtatcac ctaaccaatt gtgtattgta tatgttgctg ctaattcagt ttcctcaaaa
     4441 ttattcacca ctttacaaga attaaaacct tcttgttttg ccttcttgca taattctaaa
     4501 aatgcactca ttttttgttg tgcatcgaat tttgatttag ccggataata agctgatatt
     4561 tcattgaaaa ataagtccat agctaaataa gttttaataa taaattactc cactcatcta
     4621 acaatcctgc gggatactcg cttaatgtac cttttttatc aatgaatatt tctgtaactc
     4681 tactacgatg ttcatcatca ttaggatttc tatcaaaata ataaatggct aatttatcat
     4741 ggtctaaaac attatctttt acagctaccc tgattccgtt gataatatga tcactgtggg
     4801 attcacagaa aatttgaacg ccattttctg cagctaaagc cattagcctg cccatagccg
     4861 actgacctgt cggatgcaaa tgagattcag gattttcaat caacaatagg gacccgggct
     4921 cagcttttaa aagagatact attaaaggga gcacatataa tataccgaaa ccgacattat
     4981 aaggcgaaaa ttcatttgta aatcttgttt ttgtttcaaa ttggattcca agtctcaaag
     5041 aatcaactcc ttttaaatct tcaataacaa gtttagtgcc tgggcttatt tctcctaacc
     5101 atgctgctgt ttggtgtaat agattattag aactagcctt taagtgtcgt aaattttcta
     5161 attgaattat ttcactatta ccaaacattg aaagataatg aacagcatat tctcctttaa
     5221 taccaatgtt tttgttttgt tttacttcaa gttctgattt gggatataat ttctgagggc
     5281 catagtgttc agcggacaag tattgacaat gattattaaa tagagaaaac ccttcaaatt
     5341 ttgagatttg attgataata agattgccac catgttcaat aggtaaaata tcggttgttg
     5401 gatcaaattg aaaagcccac tgatatcttt gtcctttttg tgtctgaatt tcaaattcaa
     5461 tatgttcacc tctacctgct gattgataat aagcatcttt accagtgcca atagaaatta
     5521 gatctttgtc gttaagtaat aatcctttag tatcaagtag gcctttttga aatgattggc
     5581 gcaataacaa caatgattga agaagtgatg attttcccat accattcata ccaaaacata
     5641 agtttaggtt tgaaggtttt aatttcaggc ttttaaaagc cttgaaattt ttaatagcta
     5701 tgtatttaat catgggctaa tgaatttctt atgactgttt caattatcga aaaacgatta
     5761 ataacatttt gtttgtcagc agtaccggta cttattgctt tatccaattt tttatctgaa
     5821 gaatttagtg actccataaa ctgttcaata acaaaatctt tgttcgttat tagtttattt
     5881 ctttctgtat cggacaattt agccaaaagt acagaccaca cttcaaaaag agctttgttt
     5941 aatggcttgc gtttatcgga tttcttatat atttttctga aagcacgatt accaaaaata
     6001 gcccttgctg ctttcattga tgaaataaaa tcattggtta tagtttgtaa cctggcttct
     6061 gataagtctt tcaactcact tacagcgcta ttaaggtact cctccaaatt gcctttatat
     6121 tcagaaactg ggtgtaaata aaaggctatg aatcgggtaa caaaatcccg gtcaagcatt
     6181 ctttcggtag gtattgaatt tgttgtagct gaagtgaatt cttgaatttc tgcaagtttt
     6241 ttaatataat ctgcagcctt accctggttc aaagcatgtc ttatttctgc tggttccaat
     6301 atcagacctc ctgtattaat tcttttaaag atattatatt ttacctctgg cggtgtacct
     6361 ggatttatta tataaacagt tatctcatgt tcctcaatcc ttcgttgcag gtctcttgga
     6421 agattatcaa atttaaaacc attgaattgt gtgagatatt ccagattttg gagcgttaaa
     6481 attttattaa ttacgaagtt tttaaatgta cttaatcgct ggaggccgtc tacaacaagc
     6541 caatcgttat cattagtacc atcaaaataa aatgccggta aagggattct gataagtatc
     6601 gattcaatta agcggctttg ttttgtttca tcccaaaggt ctgatttacg ttgaaaacca
     6661 gtaagcatat ttatttcacc atttcttaaa cgacggaaaa tggcttcaag aatcatttgc
     6721 tttgtttgaa tatcaatctt ctttggatca aaaggtttcg atatttcagc aactggacca
     6781 tagtacagta actcatcttc taccagatcc ttggcgttat cgtaattttt tgttattacc
     6841 atcttattcc caataatatt tacaacattc ttttgttaac acacatgact gcctcggata
     6901 tcatggcttc ctagtactcg ctcgctaatc aattcatttt atcgctatag aatctcaacc
     6961 tataaacagt tgcttttgtt atcgattttt gacggaaaga gcctggtttc tccggatttt
     7021 tcggtacatt ccactggtcg tggattataa aagagttatg acctcacacc ttactcctca
     7081 actttcctcc gtgtttcttc cttttctatt atttttatct gtcccgatgc ctgaatagct
     7141 ttaagtgtga tctccttgga attattctct gcattctcag ctttggaata ggcctgttta
     7201 agttgaatat taaggtcatt gatctttgca atcagcgatt cgatctgttg attctttaac
     7261 tttacctctc cttcgtggtc tttaagaaga agttgttttt cgaatttgta atccttctca
     7321 agccgggaag ttacgtcctt tactgcaacc tggactgcct tctcaagttc agccgggaag
     7381 ctttctgctt ttgatctcaa tccttccagt tcttgttcag cagcagccac actttgttcc
     7441 ctgagcttaa tcccgctatc aaaggcttcc aatttttctt tcagctcttt ttcaagctgg
     7501 aacttcttct gctcatattc atccttatct tttttcctcg taagcttaag attatactgg
     7561 aattcctctt cttcacgaac ccgttgcttt ttgagggttt cctctgcttc ttttctgttt
     7621 tcttcccata acttcgtttc tttgtcgcga atcagtttct cctgcctgat ctcttcttcc
     7681 agctcgcttt ttctttgtgt cgtttcacgt tcaaattcct ccttcttttc tttctggact
     7741 gcaagaatta cagcaagtga atcggctccc gcatttatct gataaagatc cttcagcctt
     7801 tgatcctcaa ttagtattgc ttctctgatc ttagccagtt tctgacgttc agtggtgaga
     7861 tcgtcttcaa tcctgtcaag cttgctgttc aatgaaagtt tcaatgaact tatagctcca
     7921 atgatctctt catctgtcag ttttgcagca tcctccaccg tagcttcttt cactttcttt
     7981 tcctgttctg ctttcggact ttcctgtttc gaatcctgga ttctctttaa caattcatta
     8041 taagcattca gtatttctga ttttgtgttc gttaaactta ttttcttttc catggtttag
     8101 ggtttttaaa tttggtaact tctttttatt cattctttta aataatcatg gcccggaatt
     8161 tcagaaaaat atgactgtaa tattacaaag ataaatatat tcaaaccttc cacaaaaaaa
     8221 ccagatacat atatagtttt gatattctga tatttaacaa gctatccgga acagatctgc
     8281 cgctaccggg aactaaaaac atcattatct ttttacccgc tgatgtcatg ttttccttat
     8341 cagacaaatg cagaccgcag gaatgaagga tttcctgcaa taaatcctat atttgttttt
     8401 caatacgatt cctgatgaag atcaaatccg gatatatcat cattttcatt tttatttacc
     8461 tttttctgcc tgttgagcgg atatcatccc aggaaattcc ccatccggtt gccaatactg
     8521 gcatttatca gttccttgat gaactggcaa acgaccaggt agtccaactc agctccgttg
     8581 taaaaccata ctccaggctg ttcatagccc gatgcctgcg ggatgcagac gaaaaacgtg
     8641 accggctttc ccccaggcag caaagagaac ttgattttta cctgagggat ttcgggaagg
     8701 aagacagttc aaggaataag cttttcagat catctttcag cagtaacttc gaaaaagtga
     8761 gttctgcaag ggaagatctt ttttcccgtc tgataaaagt aatacgaggc aataaaagat
     8821 gggatctttt ttattattct gattctctct tctcacttac aataaatcct attattggcg
     8881 gagaattttt taccaactcc tccggaacgg caacatactg gagaaacgga atcgaggcaa
     8941 gggcatacgt caaccggtgg gggttttatg cttccctcag ggacaatcat gaaaaacc
//
LOCUS       DAOSPX010000105         8990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7382_length_8990_cov_4.059765, whole genome shotgun sequence.
ACCESSION   DAOSPX010000105 DAOSPX010000000
VERSION     DAOSPX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7382_length_8990_cov_4.059765"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLZ75_06675"
     CDS             complement(<1..193)
                     /locus_tag="PLZ75_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72120.1"
                     /translation="MKKIALTTLFLSLLILLPDKVLAQNEALFISAAGTPSAPRVPMV
                     WNKYYTNAGITDFCRKLEKA"
     gene            complement(266..2005)
                     /locus_tag="PLZ75_06680"
     CDS             complement(266..2005)
                     /locus_tag="PLZ75_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOS72121.1"
                     /translation="MNVFLRFSILALMCCFLSVSTGAQGRYGDHRVMTRTVEALAKEY
                     PSLCSVKPLGKSAGGKELVVITIGTGDRDNKPGIAVLGGIEGSHVLGRELAAGFAGYL
                     LKNSGSPEIQNLLGKITFYVIPDVSPDASGQFFSDLKYERNVNDTPVDDDRDFVTNED
                     PLEDLNNDGLITLMRVSDPAGKYMKSPDDERIMVEADFSKGETGSYLVFPEGIDNDGD
                     GLFNEDGPGGVNFNRNLTYNYEAFGAGAGAHPVSEPESIAVLDFLFDHFNIYATMAFG
                     PQDNLSQPAKPAGGRQAAAQPAEQGYIPGGQGQFQFPGAFPMQGPPPGPQSMRMMTGQ
                     ARKITSVLSQDETIIKLVSEKYRNITGVNGSPAATLPPGNFMEWSYYHYGRYSFSTPA
                     WWFPAEPGKNPGVAFLKFAEENNMENVFVPWTEIKHPGFPGKKVEVGGIKPFVTINPP
                     EDRLQDLIEKNSRFIVEMAAMHPELQFLDMKVENQGENIFRISLKVHNKGIFATCAAA
                     GERNVFTRLMRISVETNGKQKFLSGQKVQTIKRLEGGESAEYSWLIMGKGTVKITAGA
                     VNTGIITTTAELK"
     gene            2270..3148
                     /locus_tag="PLZ75_06685"
     CDS             2270..3148
                     /locus_tag="PLZ75_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOS72122.1"
                     /translation="MRKALVIFSFFFLWACGGGPSGPDEMLITVTIAPYKYFIREIAG
                     DDFTVNIMVPPGANPHIYEPYPAQIRSLSRSAAYLGNGFLGFESAWMKRFEEINKDMK
                     KVMAGDRISPIAPDHSHGAGRSHSEAADPHYWVSPKCALELAAVVRDLVCELNPASAE
                     EYNRNYETLAGKIREVDKRAEDLFSDLTNRHFMIYHPNLAYIARDYGLEEVAVEFEGK
                     EPSPSRMKELIDLAVRENINTIFIQKEYDIRNARAIAAGTGGEIRIIDPLSEDWYRSV
                     TDIIDGLYTSLTSSGK"
     gene            3158..3904
                     /locus_tag="PLZ75_06690"
     CDS             3158..3904
                     /locus_tag="PLZ75_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS72123.1"
                     /translation="MAFIFEMHSVSASYDTNTVLEGVDFSVDENDFIGVIGPNGGGKT
                     TLLKIILGLLKPSAGEIRFNPDLPDSERIGYLPQISTGDVNFPVNVTDVILSGMMIRK
                     RIFSRMSADDRKKAGRIAEELGLSGLERKALSELSGGQIQRVLLGRAIIGDPRLLLLD
                     EPGNFVDSSFESDFYGKLKELNKRMAILMVSHDIGMISSHVKSFACVNRKLHYHPSSE
                     ISNDQLLAYGCPIQLITHGDVPHTVLKTHN"
     gene            3905..4753
                     /locus_tag="PLZ75_06695"
     CDS             3905..4753
                     /locus_tag="PLZ75_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOS72124.1"
                     /translation="MEGTIFQYDFIVKGLLGALFASITAGFAGTYIVTRKMVFLSGGI
                     THASFGGIGIGYYAGINPVAGAAVFGVLSALGIEYLSINHRIREDSAIGILWAFGMAT
                     GIIFIYLTPGYSPNLMSYLFGSILTVTNADIIALGIMSVIFFIYFGVFYRTILYTSFD
                     ESFARTYSSGVDAFKYVTTSLIALTIVLNIRMAGVILVLSLLTLPPNIAMLFTRKYSL
                     IILVSILTGFAGTASGYVISYFAGIPVGATIIFTLVIMWIIAKGITMLMLSGFSNLNP
                     DCDRKQ"
     gene            4787..6178
                     /gene="lpdA"
                     /locus_tag="PLZ75_06700"
     CDS             4787..6178
                     /gene="lpdA"
                     /locus_tag="PLZ75_06700"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOS72125.1"
                     /translation="MDYDIIIIGSGPGGYVAAIRASQLKMKVAVVEKADIGGVCLNWG
                     CIPTKSLLRSAQVFDDLKHASEYGISVAGELKADFSAIIARSRSVAEGMSKGVQFLFR
                     KNNITLIRGKGRLAGANSVEIDDGEGNKTIKSAGHIILATGARSRELPGLKQDGKKVI
                     GYREAMSLPVQPESMVVVGSGAIGSEFANFYQSVGTAVTLVEFLPRLVPNEDEEVSRQ
                     LERSFKKMKMKIMTGSSVESADTSGEKCVVTIKTPKGTETVEADIVLSAVGVTTNIEG
                     LGLEEAGVRIERDKVVVDEWYRTSVEGVYAIGDIVHGPALAHVASAEGIICAEKIAGL
                     DVEALDYGNIPSCTYTSPEIASVGYTEEAARAAGYEIKVGKFPFTASGKASAAGSRDG
                     FVKLIFDASYGELLGAHMIGANVTEMISGLVIARKLESTAHEIMKSVHPHPTMSEAIM
                     EAAAAAYGEVIHI"
     gene            6323..6883
                     /locus_tag="PLZ75_06705"
     CDS             6323..6883
                     /locus_tag="PLZ75_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS72126.1"
                     /translation="MPEIRNIINGCLKGSRRDQELLYRRYAPSLYAVCLQYSDNDEEA
                     RDILQEGFIKIFTNLSHFKYEGSFEGWMRRIIVNTALEKYRSRNRLYMVEDIDMIPEP
                     FVDPDNEDYSGLDAGDLMEIIRMLPPKYRMVFNLFALEGYSHREISRMINISEGTSKS
                     NLARARMILQRRVEAYTGIKKKVLNG"
     gene            6876..8207
                     /locus_tag="PLZ75_06710"
     CDS             6876..8207
                     /locus_tag="PLZ75_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOS72127.1"
                     /translation="MDETRTNIDLVFRNGLKNYEVLPPPEAWNNIRPAIRARQRPLII
                     LRAAASAAVLLSVAFLAYRLSTRITMPEQTVISGLPEGIPPALVIAPGEVQVVAEKNV
                     PPERQIIAEADSDVPGEMEVIPGAADDNLIIQPVETSQTASGVVFPARHGRKDFSLRE
                     LFRSDRVEIAAADAEPFYFPDASESGKKTGKWRISALVSPTYYTSFYSGNDEFVSRLM
                     TDEQPGFSYSGGLALAYRVSKRVSVQSGLYFSSFGQELAGITSFAGFQPYDYAKGNRN
                     FGVMTTRGMIYTNNSDVFLLDTRNENRIVTRYTKDYFDPSKANLVYVDNSLRQNFSYL
                     ELPLIIRYKLLGRALDFNLIGGLSSNILINNSVYAAIDGGRYQVGRTEGMSMLTFSSS
                     LGMGMEYNFPGNLSLNLEPVFRYYLNPFSQTPALKVHPYSFGIFSGISYRF"
     gene            8312..>8990
                     /locus_tag="PLZ75_06715"
     CDS             8312..>8990
                     /locus_tag="PLZ75_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOS72128.1"
                     /translation="MDLPRRNIAIIATIVMTLSLLLFTTLNGFKGFGSTATGGTMDSA
                     YFNRPFRLPDEVAFAGERMPLENFDTKESLEREILVSAYRHSSTILIIKRANRYLPVI
                     EPILKKNGIPDDFKFLVAAESEYSNMISPAGATGFWQIMQSTGREHGLEINNVVDERY
                     HLEKSTQLACDFIRKSYEKYGSWTMAAASYNCGRSGLDTQISIQKKNNYYDLLLTEET
                     ARYIFRVV"
BASE COUNT         2333 a         1966 c         2266 g         2425 t
ORIGIN      
        1 aagctttttc aagtttcctg cagaaatcgg tgattccggc gttggtatag tatttattcc
       61 agaccatggg aactctggga gctgaaggtg ttcctgccgc tgagataaag agtgcctcgt
      121 tctgggcaag aactttatcg ggaaggagta ttagcagtga caggaataat gtggttaaag
      181 ctattttttt catctcgttg tattttattt ttgagcaatc agtcaatgaa tctgtatcag
      241 actgatcagc aagttgaact gatagttatt taagttcggc agttgttgtt ataataccgg
      301 tattaacagc tccggcagtg atttttacag ttccttttcc cattatgagc cagctgtatt
      361 cagccgattc cccgccttca agccttttga ttgtctggac tttctgtccg ctgaggaatt
      421 tctgttttcc gtttgtttca acgctgatcc gcatcagtcg tgtgaacaca tttctttcac
      481 cggcagcggc acaggtggca aaaatacctt tattatgcac tttaagggaa atcctgaata
      541 tgttttcacc ctggttttcc accttcatat caaggaattg cagttctgga tgcattgcgg
      601 ccatttcaac aatgaagcgc gaattcttct caataagatc ctgaagcctg tcttccggag
      661 gattgattgt tacaaacggt tttattcctc ccacctctac ttttttaccg gggaaaccgg
      721 ggtgttttat ttccgtccat ggaacgaaaa cattttccat attgttttct tcagcaaatt
      781 tcaggaatgc cacaccggga tttttcccgg gttcagccgg gaaccaccaa gccggtgtac
      841 tgaagctgta tctgccataa tggtaatatg accattccat gaaatttccc gggggaagcg
      901 ttgcggcggg tgaaccgtta acgccggtta tattccggta tttttccgac accagcttaa
      961 tgatggtttc atcctggctc agaacactgg ttattttcct ggcctgtccg gtcatcattc
     1021 tcattgattg cggtccgggg ggtggtccct gcatggggaa tgcccccggg aactgaaact
     1081 gaccctgtcc gccgggaata tatccctgtt ccgccggctg ggctgcggcc tgacgtccgc
     1141 cggcaggttt tgccggctgg ctgaggttgt cctggggtcc gaatgccatg gtggcataga
     1201 tattgaaatg gtcaaaaagg aaatccagga cagcaatgga ttccggttcc gatacaggat
     1261 gagctcctgc tccggcgcca aaagcttcgt agttgtatgt caggttcctg ttgaagttca
     1321 caccgccggg tccgtcttca ttgaagagac cgtccccgtc gttgtcgatg ccttccggaa
     1381 agacaagata agatcctgtt tccccctttg aaaaatctgc ttcaaccatt attctttcat
     1441 cgtcagggct cttcatatat ttcccggccg gatcgctgac tctcatcagt gtaatcagtc
     1501 cgtcgttgtt cagatcctca aggggatcct cgttggtgac gaaatccctg tcatcatcaa
     1561 caggcgtatc attgacgttt ctttcgtatt tcaggtcgct gaagaactgc ccggaagcat
     1621 cagggctgac atccggaatc acgtagaagg tgattttccc gagaaggttt tgtatttccg
     1681 gggatcctga atttttcaga aggtatccgg caaagccggc agcgagttcc cttccgagca
     1741 cgtgacttcc ctcgatgcct ccgagaacgg ctattccagg cttattgtcc ctgtcgcccg
     1801 ttcctattgt gattaccacc agttccttcc ctcccgcact tttgcctaga ggctttacag
     1861 aacaaaggga gggatattcc tttgccagtg cttcaactgt acgggtcatc actctgtgat
     1921 ccccgtacct gccctgagcc cctgtactga ctgaaaggaa gcagcacatc agggccagaa
     1981 tggaaaatct caaaaaaaca ttcatgttat ggtctttaag ttatcaatgg tccccggcta
     2041 agtccgggag atccggaagg cttaaaatta acgcatatta ttttaattgc aacacctaaa
     2101 ctttttattg tcttatatgt taattttgta gtcaggcaga tggggatcag gatggaatac
     2161 tgccctgaac cgccggatcc tgcggttatt ctgcaggcag gcacagccgg gaccggcctg
     2221 aatggtttat taatgatagt cttgcagtga ggcagaagct gttagttcga tgagaaaagc
     2281 actggtaatt ttttcgtttt ttttcctgtg ggcctgcgga ggcggtcctt caggtccgga
     2341 tgaaatgctg ataacggtca ccattgcccc gtataaatat ttcatcaggg agattgccgg
     2401 ggatgatttc accgtaaaca taatggtgcc tcccggtgca aatccccata tctatgagcc
     2461 ttatcccgcc cagataagaa gtctgagcag gtcggccgca tatcttggga atggttttct
     2521 cggcttcgag tcggcatgga tgaaaagatt tgaggagatc aacaaggaca tgaagaaggt
     2581 aatggccgga gacaggattt cacccatagc tccggaccat tctcacggag ccggcagaag
     2641 ccattccgaa gctgcagatc cccattactg ggtatctccg aaatgtgccc ttgaactggc
     2701 tgcggttgtc agggatctgg tatgtgaact gaatccggct tcggctgaag agtataacag
     2761 gaattacgaa actcttgcag ggaagatcag agaagttgat aaaagggctg aggatctttt
     2821 ttcagatctg acaaaccgtc atttcatgat ttatcatccc aatctggctt atattgcaag
     2881 ggattacgga ctggaagagg ttgctgtgga atttgagggg aaggaacctt ccccctcacg
     2941 catgaaggag cttattgatc tggcggtaag agaaaacata aacacgatat tcatacagaa
     3001 ggaatatgac atcaggaatg ccagggctat tgctgccggc acgggaggag aaatcaggat
     3061 aattgacccg ctttcggaag actggtacag atccgtaacg gatatcattg acggcttata
     3121 cacaagtctg acaagcagcg ggaaataagt tgagcatatg gcttttattt ttgaaatgca
     3181 ttctgtatcc gcctcatacg acacgaacac cgtgcttgaa ggggttgatt tcagtgtgga
     3241 tgaaaatgat tttatcggag tgatcgggcc gaacggcggg ggtaaaacca ctcttctgaa
     3301 aatcattctt ggactgctga agccttctgc cggggagatc cgttttaatc cggatcttcc
     3361 ggactcggaa agaatcggtt atcttcccca gatttcaacc ggtgatgtga attttccggt
     3421 taatgtgaca gatgtcattc tttcgggaat gatgatcaga aagaggattt tctcaaggat
     3481 gtcggcggat gacaggaaga aggccgggcg gattgcggag gagctcggac tttccggact
     3541 tgagaggaaa gcgctgagtg aactttcagg aggacagata cagcgggttt tgctcgggcg
     3601 tgccataata ggtgatcccc ggcttttact tctggatgaa ccgggaaatt ttgtcgattc
     3661 ttcgtttgag agcgattttt acggaaaact gaaggaattg aataagagga tggcaatact
     3721 catggtgtcg catgatatag gaatgatctc ctcgcatgtg aaatcctttg cctgtgtaaa
     3781 caggaagctt cactaccatc cttcctcgga aataagcaat gatcagctcc ttgcgtacgg
     3841 atgtcctatt cagcttatta ctcatggtga tgtgccgcat acggtactta aaacacataa
     3901 ctgagtggaa ggaactattt ttcaatatga ttttattgtc aagggtttgc tgggagcgct
     3961 ttttgcaagc ataacggcag gatttgccgg aacatacatt gttaccagga agatggtttt
     4021 tctcagcggg ggaatcactc atgcctcatt cggagggata gggatagggt attatgccgg
     4081 gatcaatcct gttgcaggag cagcggtatt cggagttctt tcagcactgg gaattgaata
     4141 tctttccatt aaccacagga taagggaaga ttccgccatt ggtatcctgt gggcattcgg
     4201 aatggctacg ggcataatct tcatttatct tacaccgggc tattcgccga atctgatgag
     4261 ttatcttttc ggaagcattc ttaccgtaac caatgccgat ataattgcac tgggaataat
     4321 gtcggtgata ttcttcatct atttcggagt attttaccgg acaatactgt acacatcctt
     4381 cgatgaatcc tttgcacgta cctactcatc gggtgttgat gcattcaaat atgtcaccac
     4441 atcgctaatt gcactcacca tcgtacttaa catcaggatg gccggggtga tccttgtgct
     4501 ttcacttctt accctgccgc cgaatattgc aatgttgttt acccggaagt attcgcttat
     4561 aatccttgtt tccattctca ccggatttgc cggaactgct tccggatacg tcatttcata
     4621 tttcgccggc attccggtgg gggctacaat aatattcacc ctggtgataa tgtggattat
     4681 tgcaaagggt atcaccatgc ttatgttatc cggattcagc aacttaaacc cggactgcga
     4741 tcggaagcaa tagaaaaaaa tataaacaaa tttaatctgg aaaaaaatgg attatgacat
     4801 aataataatc ggaagcggcc ccggagggta cgtagctgca atcagggcat cgcagctgaa
     4861 gatgaaggta gctgttgttg aaaaagcaga tatcggagga gtctgcctca actggggttg
     4921 catcccgacg aaatcactcc tgaggagtgc gcaggtattt gacgacctga agcatgcatc
     4981 tgaatatggc atttcagttg ccggagagct gaaggctgat tttagcgcaa tcattgcccg
     5041 cagccggagt gttgcggaag gaatgagcaa gggggttcag ttccttttca gaaaaaacaa
     5101 tattaccctt atcaggggga aggggcggct ggccggtgcc aacagtgttg agattgatga
     5161 tggtgaagga aacaaaacca taaaaagtgc cgggcacatc atcctggcaa ccggggcaag
     5221 gtcaagggaa ctgcccggac tgaagcagga cggaaagaag gttattggtt accgtgaggc
     5281 catgagcctg cccgttcagc cggaatcgat ggtggttgtc ggctcgggtg caataggaag
     5341 tgaattcgct aatttctacc agtctgtcgg aactgccgtg accctggtgg aatttcttcc
     5401 gcggctggtt cccaatgagg atgaggaagt gtccagacag ctggaacgtt ccttcaagaa
     5461 gatgaaaatg aaaataatga ccggatcatc tgtggaatct gcagatacat cgggggaaaa
     5521 atgtgttgtg acaataaaaa ccccgaaggg aaccgaaacg gttgaagccg atattgttct
     5581 ttccgccgtt ggggtgacaa caaatattga aggactggga cttgaggagg cgggagtaag
     5641 gattgaaagg gacaaggttg ttgtggatga atggtacagg acctctgtgg aaggggtata
     5701 tgccatcggg gatattgttc acgggccggc actggcacat gtggcatcgg ctgagggaat
     5761 aatctgtgcg gaaaagatag ccggcctgga tgtcgaagcg cttgattacg gcaatatacc
     5821 ttcctgcaca tataccagtc ctgaaattgc atcggtgggt tacactgaag aagctgccag
     5881 ggctgccgga tatgaaataa aggttggcaa atttcctttt acggcttcag gcaaggccag
     5941 tgcagcaggc agcagggatg gtttcgtgaa actgattttc gatgcctcgt atggtgaact
     6001 tctcggagca cacatgatag gagccaatgt aacggaaatg atttccggac ttgttattgc
     6061 aaggaagctt gaaagcacgg ctcatgagat aatgaaatct gtccatcctc atccgactat
     6121 gtcggaggcc atcatggaag ctgcggcagc cgcatacgga gaagttatac atatctgatt
     6181 tcctgttcat tgtaacagga gaggcaaaaa gaacagagcc aacgcaacct ttttccggtt
     6241 ttcagcatca gttttaagga agaatattgg ctttcaggca gaattttgca tatttttata
     6301 ctctgtgcca agaaactata acttgcctga gattagaaat atcattaacg gttgtctgaa
     6361 aggaagcaga agggatcagg aacttctgta caggcgctat gcaccctcac tttatgccgt
     6421 ctgtcttcag tattccgaca atgatgaaga agcaagggat attttgcagg aggggtttat
     6481 aaagattttt acaaatcttt cccacttcaa atatgaaggt tcttttgaag gctggatgcg
     6541 gagaatcatt gtcaataccg cacttgagaa atacagaagc aggaacaggc tttacatggt
     6601 tgaagatatt gacatgatcc cggagccctt tgttgatcct gacaatgagg attattccgg
     6661 acttgacgca ggtgatctga tggagatcat caggatgctg ccccccaagt acaggatggt
     6721 tttcaacctc tttgcacttg aaggctattc acacagggaa ataagccgga tgatcaacat
     6781 ttcggaaggg acgtcaaaat ccaatcttgc ccgtgcacgg atgatacttc agcgcagggt
     6841 tgaggcctat accgggataa agaaaaaggt actgaatgga tgaaaccaga acaaatattg
     6901 atcttgtttt caggaacggc ctgaaaaatt atgaggttct gcccccgccg gaagcctgga
     6961 ataatatcag gcctgccata agggcaaggc agaggcccct tatcattctc agggccgcag
     7021 cttcagcagc cgttctcttg tccgtcgcct ttctggccta caggctgagc actcgtatca
     7081 ctatgcctga gcagactgtt atttccggtc tgccggaggg gatacctccg gcacttgtca
     7141 tagctccggg tgaagtacag gttgtagctg aaaaaaatgt ccctcctgaa aggcagataa
     7201 ttgcagaggc agacagtgac gttccggggg aaatggaagt gattcccggc gcagctgatg
     7261 acaatctgat aatacagcct gttgaaacaa gccagacggc atccggtgtg gttttcccgg
     7321 cccgccatgg ccggaaggat ttcagtctgc gtgaactgtt ccgttcagac agggtggaaa
     7381 ttgctgccgc ggatgcggaa cccttctatt ttccagacgc atcagagagc ggaaagaaaa
     7441 cgggcaaatg gagaatatca gcccttgttt cccctaccta ttacacaagt ttctattccg
     7501 gcaatgatga attcgtctca cgccttatga ctgacgaaca gcccggattc tcatattcag
     7561 gaggtctggc ccttgcttac agggtaagca aaagggtaag tgtccagtca ggcctttatt
     7621 tttcatcatt cggccaggag ctggcgggaa tcacatcctt cgcgggtttt cagccgtatg
     7681 attatgcaaa gggaaaccgc aatttcgggg tcatgaccac caggggaatg atctatacaa
     7741 acaattctga tgttttcctc cttgacaccc ggaacgaaaa caggattgtt accagataca
     7801 caaaagacta ttttgatcct tcaaaggcaa atctcgttta tgtcgataat tccctgcggc
     7861 agaacttcag ctatcttgaa ctgcctttga ttataagata taagctgctg ggcagggcac
     7921 ttgattttaa ccttatagga ggcttgtcct caaatattct cattaacaac tcagtatatg
     7981 ccgctattga cggaggcagg taccaggtgg gccggaccga gggaatgagc atgctcacat
     8041 tcagcagttc cctgggtatg gggatggagt ataacttccc cggcaatctt tccctgaatc
     8101 ttgaacctgt tttcagatat tatctcaatc ctttcagcca gacacccgca ctgaaggttc
     8161 atccatattc attcggcata ttttcaggta tttcatacag gttctgattc cggaacaatt
     8221 ttccctttat ctgaacactt ccggcacagg gagatatttt tatcaagctg aaaagttgta
     8281 cctttgcact ttgtttttaa tacaggacat tatggatctt ccgcgaagaa atattgccat
     8341 cattgcaaca attgtcatga cattgtcatt gcttctgttc acgacactga acggattcaa
     8401 gggtttcggc agtactgcca cgggagggac catggattcc gcttatttca acaggccttt
     8461 caggttacct gatgaggtgg cctttgcagg ggaaaggatg ccccttgaga atttcgacac
     8521 aaaagaaagt cttgaacgtg aaattcttgt cagcgcctac cgtcattcct caaccatact
     8581 catcataaag agggccaatc gttatcttcc ggttatcgag cctatactga aaaagaacgg
     8641 tattcctgat gatttcaagt tcctggtggc tgcagagagt gaatacagta acatgatatc
     8701 accggcgggc gcaacaggtt tctggcagat aatgcagtca acgggaaggg agcacgggct
     8761 ggagatcaac aatgtggtgg atgagaggta tcatcttgaa aaatccacac agctggcatg
     8821 cgattttatc aggaaatcct atgagaaata cggcagctgg acaatggcag cagcttccta
     8881 taactgtggc aggtcaggtc ttgacacaca gatttccatt caaaagaaga acaattatta
     8941 tgatttactg ttaaccgaag agactgccag gtatattttc agggttgtgg
//
LOCUS       DAOSPX010000106         8923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7458_length_8923_cov_4.931326, whole genome shotgun sequence.
ACCESSION   DAOSPX010000106 DAOSPX010000000
VERSION     DAOSPX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7458_length_8923_cov_4.931326"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1369)
                     /locus_tag="PLZ75_06720"
     CDS             complement(17..1369)
                     /locus_tag="PLZ75_06720"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS72129.1"
                     /translation="MTKRKLTRRQAITAVSAGALGSFVNWSFPSFTIKSTDQQKLAIN
                     GGEKIHRGSWPDWPVWDQAAEKNIAEMLRSGRWWRGEGEHVADFEKRYAELMGVKRCL
                     ATASGTTALIIAQKALGVDAGDEILVSPFTFIASYNVIFMNKALPVFVDTDPETFLMD
                     PSKIESRITERTTAIIPVHIYGLPVDMDRVNEVARKHRLKVIEDACQGWLGEYKGKKL
                     GSLGDAGCFSFQNSKNLPAGEGGAIISNNDELMDKCTSLHNCGRPFGTVARTSAYPIA
                     GTNFRMQQIQALILMSQMNRIRKDADIRLANALYLDASLKDIPGIIPAKLVGENCRSA
                     YHLYAFRYISKEFNDIPKARFMNALQAEGIPCSSGYGPQNRDGLIEEQLNSKGYKRLF
                     SEARLKQWREENVLPGNDKLCSEAVVFTQNILLGSRSDMEDIVRAIRKIYQNRDSLKA
                     "
     gene            complement(1464..1841)
                     /locus_tag="PLZ75_06725"
     CDS             complement(1464..1841)
                     /locus_tag="PLZ75_06725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOS72130.1"
                     /translation="MNTGKFSPRSRLKSFKYAFRGLGSLLKFEHNSRIHLFAAIIVVI
                     LGIILKVSVTEWCILILAIGFVFFAELLNSSAEAFSDAVKPGWDEKIMKAKDYASAAV
                     LVAAITSAVIGILIFLPRLILLF"
     gene            complement(2061..5666)
                     /locus_tag="PLZ75_06730"
     CDS             complement(2061..5666)
                     /locus_tag="PLZ75_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOS72131.1"
                     /translation="MRLRNAYSGLKKSGYLRGIMIIGIISALLFPIIATSPLKAQDLP
                     EYDEIGIFLIIPRLGGIEMDAVIRNDELYLPVQDLFDFLKIRNIPSPGMETISGFFIS
                     PEAKYEINRRDNVITYQNKSYQIKPGDLIRTESNLYLHASYFGNIFGLECRFSFRSLS
                     VTVESKLELPLIREMRQEEMRRNLTRLKGEIKADTLIGRSYPWFRFGVADWSAVVSEQ
                     TDGKTDTRLNLALGSMIAKGEFNAFLHYHDQKAFSIKQQNYLWRYVNNDFSPLRQILA
                     GKINTNSISTIFNSVIGIQLTNTPTTYRRSFGSYTLSDFTEPGWIVELYVNNVLVDYV
                     TSDASGFFTFQVPLVYGNTVVKLKFYSPWGEERTKEQNITIPYNFLPVGTFEYNVGAG
                     VVEDGLQSRFSRARINYGLTRSLTVGGGMEYLSSLGSGQFIPFLNTSFRLTNNILLFG
                     EYAHGVRTKGTLSYRMPSNLQLDLNYTWYEKDQQAIHFNYREERRATLSFPLKIAGLK
                     SFQRFSYNQIVLPSSDYKTAEWMFSGTLFGINTNLTTYGIFLEKAEPNLYSNLSLAFR
                     LPSNFILMPQAQYSYSRNEFISAKTRIEKRFLEHGHINLSWEYNFRMSVGLSELGVRY
                     DFSFAQLGSSFRLYGKKPSFTQYARGSLVNDRQTRYLKADNRTNTGRGGISIVPYLDL
                     NSNNRRDPGEPPAHGLNLRTNSGRVEKSDKDTIIYILGLEPYTNCFIELDPNSFENIS
                     WRLNQKSLSVSVDPNIIKKIEVPVMVAGEATGTVELDENGIRKGLGRIIVSFETGTRK
                     AAGRVLSEEDGYFSFFGLSPGKYTVRIDTAQLAKLNMTARPDSITFDVAGGIDGDFVE
                     GLDFVLTRKQLETIPVIEAGEGRPFTRKDTTYMVVHEVVEELVTIAEDSWAIQLGAFR
                     VRTNAERYRRTLEKLLNRKVDIVIEDNYWKVRIPDIKTRDDVDNVLELLRRNGVTEVW
                     VVRLKAKQQQLILKERQDTVISITETTELISEPSFDRAASVELGVFDNTADAIALKDT
                     LLKSVRMPVLIIRDEGLYRLRVTGFANWEEMMEFISSLDKSRYPDMRIHTLSRSSEKV
                     TVPEKPVEIQADTITVPAEKIPEQPEPEETVVKKEPTVALQAGIFHKKSKAQKAVRKI
                     SARLRLPVEIVLQWDYYHVIITGFYSREETYMYFPELAALGYPGVTVIENYKPEK"
     gene            complement(5697..6209)
                     /locus_tag="PLZ75_06735"
     CDS             complement(5697..6209)
                     /locus_tag="PLZ75_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOS72132.1"
                     /translation="MTELRYKSIRVRKLIFITAAVIFLSGIWNVAKAQEDPPRPPSIT
                     LARNLSFGAFYTTTGGTVTVDQYDTRTSFNVTLFTFSSISAARFNIYANRGTIINILN
                     IPDFQLTNGSYSMTVSITATNPLLPFVNDNTYVVPTVLTMGATLTVGAPVSNPPGNYS
                     GTFDVTLVIE"
     gene            complement(6212..>8923)
                     /locus_tag="PLZ75_06740"
     CDS             complement(6212..>8923)
                     /locus_tag="PLZ75_06740"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS72133.1"
                     /translation="GGQFDLICTVTDNRGCTGTASRTVTVFPAPKADAGPDISGLCTG
                     TMPIAMTGASASGSYSGTPVWSGAGGTWTQNPDPALATFTPSTPSGSTTATLTLTGAN
                     GCSNSTDERLITWNSIPGQPGFFTESSITVCRGQSAVVFTVPHDPAATSYNWTYTMGS
                     GVTISGNGNSVTLDFSPAATSGRLSVSAVNSCGASIPRSVDIVVTIPPVATFIYPGNP
                     YCPMGTNPFPVFTGGGVPGYFSSTPGLVFTDHSTGQVDLSASTPGDYIVTNTIAATGG
                     CPAVTATSTISIISEFVWSGSADSDWNNPANWSCGVVPSAAYSVLIGNVTNKPVLSTG
                     AAGAIKNLTIENGSSLTVTENTIRITGTITNNGVFEAGEATVELAGTMPQTINSSAFT
                     GNTVSGLTVNNISGVTLNGELKVSGIVLIQNGTLNSNGNLTLLSTAGRTAMIDGSGSG
                     GINGNVTMQRYLPSGFGYRYISSPFSNASVNQLADYLKLGSAFPMLYKYDENRMLGGI
                     PATGWVRYNYPDSLLRPLHGYSANFGSLSGDTTFDVTGTVNNGYISRTLYNHNNPYTT
                     GFNLVGNPYPSPVDWNAPSGWTKTNIDAAIYYFVASTTDQYGGTYRSYVGGIPVDTLA
                     TGIIPSMQGFFVHVSEGSYPVSGTLAMDNRVRVIDLKRRFAKSSKTGSTPLLRISAEF
                     SDDRLSSDPSVIYFSENAETDFNGELDALKLMNTDLAVPNLYSIASDGSKLSINALPS
                     SLVSGCKIPLGLRISRTGNVIFSVSQLDPMMGITSVYLSDAVEDISQDLMPGNRYSVS
                     LDPGEYSNRFFIYLNTIPTGMEHVSTDELSFRTYYTRGTLVTDIGLGSAAKALMTVTN
                     ISGNVKLYETYYESGHYEHNINLIPGIYIVTVTCENRRITRKITVWK"
BASE COUNT         2274 a         2048 c         2023 g         2578 t
ORIGIN      
        1 atttttttca ttttcttcag gccttcagtg agtccctgtt ctggtagatc ttcctgattg
       61 ccctgacaat gtcttccatg tcactccggc tgccaagaag gatattctgg gtgaaaacga
      121 ctgcttcact gcagagtttg tcattacccg gaaggacatt ctcttccctc cactgtttca
      181 gccgtgcttc ggaaaacagt cttttgtatc ctttcgaatt aagctgttct tcgataagcc
      241 cgtccctgtt ctgcgggcca tagccgctgc tgcagggtat gccttcagcc tgaagggcat
      301 tcatgaatct ggctttgggt atatcgttga attccttgct tatatacctg aatgcataga
      361 ggtgataggc agaacggcag ttctccccaa caagtttcgc agggatgatt ccgggaatat
      421 ccttaaggct ggcatcgaga taaagtgcat ttgcaagtct gatatcggca tctttcctga
      481 tacggttcat ctgcgacata aggatcagtg cctgtatctg ctgcattctg aaattggttc
      541 ctgctatcgg ataggccgat gtccgtgcaa cagtgccgaa gggtcttccg cagttgtgaa
      601 gggaagtaca cttatccatc agttcgtcat tattgctgat aatggcacca ccttccccgg
      661 cgggaagatt tttggaattc tggaagctga agcagcccgc atcacccagt gaccccagct
      721 ttttgccctt gtattcaccc agccagccct gacatgcatc ttcaatcact ttcagcctgt
      781 gtttccgggc cacttcattt accctgtcca tatcaaccgg gagaccgtaa atatgtaccg
      841 gaatgatggc tgttgtccgt tccgtgatgc gggattctat ttttgacgga tccatcagga
      901 aagtttccgg atctgtatca acgaacaccg gaagggcttt attcatgaaa ataacattat
      961 agctggcaat gaaggtaaaa ggtgaaacga gtatttcatc cccggcatca accccaagag
     1021 ctttctgggc aattataagt gctgttgttc cgctggcggt tgcaaggcat ctttttactc
     1081 ccatcagttc ggcatatctt ttctcaaaat cagccacatg ttcaccttcg cctctccacc
     1141 atctcccgga gcgaagcatt tcggcaatat ttttctcggc agcctggtcc cacacgggcc
     1201 agtcaggcca gctgcccctg tgtatcttct caccgccatt tattgccagt ttctgctgat
     1261 cagtactttt tatggtaaat gacggaaatg accagttgac aaatgacccc agggctcctg
     1321 ccgatacggc tgtaatagcc tgacgacgtg ttaattttct ttttgtcatt ttaaaaataa
     1381 ttagtttggt tgtaaggtta gactctgagt aattcctgcg ttttataaaa aacggctttg
     1441 ccgctataag ttacgaatca attttaaaat aacagtatga ggcgtggaag aaaaataaga
     1501 attccgatga ctgccgaggt gatggcagcg acaagtactg cagctgaggc ataatccttt
     1561 gccttcatta tcttttcatc ccatccgggt ttaaccgcat ccgaaaaagc ctctgcagat
     1621 gaattgagaa gttctgcaaa aaatacaaag ccaattgcca ggataagaat acaccattcg
     1681 gttacactga ctttcagaat aattcccaga atgacaacta ttatggctgc aaacagatgt
     1741 atcctagagt tgtgttcaaa tttaagcagt gaaccgaggc ctctgaatgc atacttaaaa
     1801 ctcttcagcc gcgacctggg ggaaaactta cccgtattca tataaaggat ttttcaattg
     1861 aaggacctaa aaccaatttc agactgaaag ccggtaataa aaccacagcc catacaaatt
     1921 tagaaatatg aaccgactta tgaaagtgaa tttccggctt ctcagcagag cactgcagag
     1981 gcaaatgacc ttttcaggaa aaccgtacca attctgacct gtttccggcc aaaacggaaa
     2041 ttatcggaat gccgtatcag ttatttttcc ggtttatagt tttcaatcac agtgacaccc
     2101 ggataaccca gtgcggccag ttcggggaaa tacatatatg tttcctccct gctgtaaaag
     2161 ccggttatta ttacatgata ataatcccac tgaagcacaa tttcaaccgg gagtctgagt
     2221 ctggccgata tctttctcac agccttctgt gccttcgatt ttttgtggaa tattccggcc
     2281 tgcagggcaa cagtcggttc ttttttcaca actgtctctt caggttcagg ctgttccggt
     2341 attttctccg ccggaactgt tatagtgtct gcctgaatct ccaccggttt ttcagggaca
     2401 gttacttttt cagaagatct gctgagggta tggatccgca tatcaggata tctggatttg
     2461 tcaagtgatg atatgaactc catcatttcc tcccagtttg caaatccggt gactctgagc
     2521 ctgtacaggc cctcgtcgcg gattatcagt acaggcatcc tgacagactt aagcagagtg
     2581 tctttcagtg cgatggcatc tgctgtattg tcaaatactc ccagttcaac cgaagctgca
     2641 cgatcaaatg aaggttcaga gattaattcg gttgtttcag taatacttat taccgtatcc
     2701 tgtctttctt taagaataag ctgctgctgc tttgccttga gtctgaccac ccatacctcg
     2761 gtgactccgt tccttctgag cagttcaaga acattgtcaa catcatccct tgttttaata
     2821 tcgggtatac gtaccttcca gtaattgtct tcaataacaa tatcgacctt cctgttcaga
     2881 agtttttcca gggttctcct gtaccgctcg gcatttgttc tcaccctgaa ggcaccgagc
     2941 tgtattgccc aggaatcctc agcaatagta acaagttcct caacaacttc atggacaacc
     3001 atgtaggtgg tatccttacg ggtgaagggt ctgccctctc cggcttcgat tacgggtatt
     3061 gtctccagtt gcttccttgt gaggacaaaa tccagtcctt caacaaaatc cccgtcaatt
     3121 cctcctgcca cgtcaaacgt aattgagtca ggccgggcag tcatattcag ctttgctagc
     3181 tgggcagtat caattctgac agtatattta ccgggtgaaa ggccgaagaa gctgaaatac
     3241 ccgtcttcct cgctcagtac ccgtccggca gccttccggg taccggtttc aaagctgact
     3301 attattcgtc ccagtccctt cctgataccg ttttcatcaa gttcaactgt tcctgttgcc
     3361 tcacctgcaa ccattacagg aacctctatc tttttaatga tgttggggtc gaccgacacg
     3421 ctcagtgatt tctgattcag ccgccaggag atattttcaa agctgttggg atcaagttca
     3481 ataaaacaat tcgtataggg ttccagacca aggatatata tgatggtgtc cttatcgctt
     3541 ttctcaacac gcccgctgtt tgtacgaaga tttagtccat gagcgggtgg ttccccggga
     3601 tctcttctgt tgtttgaatt aagatcgaga taaggaacta ttgaaattcc tcccctgcct
     3661 gtatttgttc tgttgtcggc tttcagatac ctcgtttgcc tgtcatttac aaggcttcct
     3721 cttgcatatt gagtgaatga gggctttttg ccataaaggc ggaatgaaga acccagctgg
     3781 gcaaaggaaa aatcataacg gactccaagc tcggacaggc cgacggacat tctgaaatta
     3841 tattcccagg agagatttat gtggccatgt tcgagaaagc gcttttctat tcttgttttg
     3901 gcactgataa attcattcct gctgtatgaa tactgtgcct ggggcattaa aataaaattt
     3961 gaaggcaacc tgaaggccag tgaaagatta ctgtagaggt ttggttctgc tttttccagg
     4021 aagataccat aagtggtaag attggtattt ataccgaaaa gagtaccgga aaacatccac
     4081 tcggctgttt tatagtcaga tgaaggcaac actatctggt tatagctgaa tctctggaag
     4141 gattttaatc cggcgatttt caggggaaat gaaagtgttg cccttcgttc ttccctataa
     4201 ttgaagtgga tagcctgctg gtctttttcg taccaggtat aattaaggtc cagctggagg
     4261 ttggaaggca tcctgtagct cagtgtccct tttgtcctta ccccgtgggc atattcgcca
     4321 aatagaagga tattgtttgt aagcctgaat gatgtattga gaaagggaat gaactgtcct
     4381 gaaccaagtg atgaaagata ttccatacct ccgccgacag taaggctgcg ggtgagtccg
     4441 taattgatcc tggcacggct gaatctgctc tgcagaccgt cttcaacaac tccggcaccg
     4501 acattgtatt caaaggtgcc taccggcagg aaattatatg gaatggtgat attctgttcc
     4561 ttcgtacgtt cttcacccca ggggctgtag aatttgagct tcacaaccgt atttccataa
     4621 acaagaggaa cctggaaagt aaaaaatcct gatgcatcag aagtaacata atcaaccagc
     4681 acattattta cgtagagctc gacaatccag ccgggttcag taaaatcgga aagagtatag
     4741 gaaccgaagg atcttctgta ggtggtaggg gtatttgtca actgtatacc aataacggaa
     4801 ttgaaaatgg tggaaataga gtttgtgttt atctttccgg ccaggatttg tctgagggga
     4861 ctgaaatcat tgtttacata acgccacaga taattctgct gttttatgga aaaggctttc
     4921 tgatcgtgat aatggagaaa agcgttgaat tctcctttgg cgatcattga ccccagggca
     4981 aggtttaatc ttgtatcagt tttaccgtct gtttgttccg aaacgactgc tgaccagtca
     5041 gccactccga atctgaacca cggatagctc ctccctatca gggtatcagc cttaatttca
     5101 cctttcagcc gtgtaagatt gcgtctcatc tcctcctggc gcatttcccg tatcagaggt
     5161 aattccagtt tgctttccac cgttactgaa aggcttctga aactgaatct gcattccagt
     5221 ccgaatatat tgccaaagta tgaagcatgg aggtagagat tggattcagt acgaatgagg
     5281 tcaccaggtt ttatttgata acttttgttc tgataggtga ttacattgtc acgtctgttt
     5341 atctcatatt ttgcttccgg gctgataaaa aatccgctga ttgtttccat acccggagaa
     5401 ggaatattcc ttattttcag gaaatcaaaa aggtcctgta caggaagata aagttcgtca
     5461 ttccttatta cagcatccat ttctataccc ccgagccggg gtatgatcag gaagatacct
     5521 atttcatcat attccggaag atcctgggct tttagaggag atgtagcaat tatgggaaac
     5581 agtaatgctg aaattatgcc gatgatcatt attccacgca gataccctga tttcttcaat
     5641 ccggaatatg cgtttctcag tctcatttat ttcagccttt taattctccc agttaattat
     5701 tcaattacca gcgttacatc gaaagtgccc gaatagttac cgggcggatt ggacacagga
     5761 gccccgacag taagagtagc tcccatagtt aatacagtag gcaccacgta tgtgttgtca
     5821 tttacaaaag gcagaagcgg atttgtagct gtaatgctta cagtcattga atacgagccg
     5881 tttgtaagtt gaaaatccgg aatgttaagt atatttataa tagttcccct gttggcataa
     5941 atattgaagc gggcagccga aatactgctg aaagtgaata aggtaacatt aaatgacgtt
     6001 cttgtgtcat actgatcaac cgtaaccgtt cctccggtgg tagtgtaaaa ggcaccgaag
     6061 ctgagattcc ttgccagggt tattgaagga ggtcgtggcg gatcttcctg tgcttttgcc
     6121 acgttccata ttccggacag gaaaattacg gctgcagtaa taaaaatcag cttcctgact
     6181 ctaatgcttt tatatcttaa ctcagtcatt ttcatttcca gacagtgatc ttccttgtga
     6241 tccttctgtt ttcacacgtt actgtaacaa tgtaaatacc cggaatgaga tttatgttat
     6301 gctcataatg tccggattca taatatgttt cgtatagttt tacattaccg ctgatatttg
     6361 tgaccgtcat cagtgcctta gcggcagaac ccagaccaat atcagtgacc agtgttcctc
     6421 tcgtataata tgttctgaaa gacaattcat cagtacttac atgttccata cccgttggaa
     6481 ttgtattcag atagataaaa aagcggttgg aatattcccc cggatcaaga gaaactgaat
     6541 accggtttcc gggcatcaga tcctggctga tatcttccac agcatcggaa aggtatactg
     6601 atgtgatgcc catcatggga tcaagctgcg aaacactgaa tatgacattt cctgttctgc
     6661 ttatccttaa gcccagagga attttacatc ctgaaaccag ggaggagggc agtgcattta
     6721 ttgatagttt cgaaccgtct gatgctattg aataaagatt cggaaccgcc agatccgtat
     6781 tcatcagttt cagtgcatca agctcaccgt tgaaatccgt ttctgcattt tcactgaaat
     6841 atattacaga tggatctgaa gaaagccggt catcggagaa ttctgcgctg atcctcagta
     6901 agggcgttga tccggtcttt gaagatttgg cgaaacggcg cttcagatca atgactctca
     6961 ctctgttatc cattgcaagt gtgccgctca ccggataact gccctctgaa acatgaacaa
     7021 agaacccctg cattgaagga attatgccgg ttgcaagagt gtcgaccgga ataccgccga
     7081 catagcttct gtaagttccc ccgtactggt ctgttgtact ggcaacgaaa taatatatgg
     7141 cagcatcaat gttcgttttt gtccagcccg atggtgcatt ccagtcaacc ggcgagggat
     7201 agggattgcc aaccaggttg aaacccgtgg tatatgggtt gttatgattg tacagggtcc
     7261 ttgatatgta gccgttgtta accgttcccg taacatcaaa ggttgtatca ccggaaagtg
     7321 agccaaaatt ggccgaatat ccgtgcaacg gtctcagaag gctgtccgga taattgtatc
     7381 ttacccatcc tgttgcagga attcctccaa gcattctgtt ttcatcatat ttatagagca
     7441 tcggaaaagc tgatccgagt ttcaggtaat cagccagctg gttaacggat gcattgctga
     7501 agggagagct gatatacctg tatccgaatc ctgaagggag atagcgttgc attgtaacat
     7561 tcccgtttat cccgccggag cctgaaccat cgatcattgc cgttctgccg gccgttgaca
     7621 ggagcgtcag gttaccgttg gaattcaatg ttccgttctg aatcagtact atgccgctta
     7681 ctttgagttc cccgtttaat gtaacccccg agatattatt caccgtaagc ccgctgactg
     7741 tgtttcctgt aaaagctgat gaatttatcg tctgaggcat ggtgccggca agctcaactg
     7801 tggcttcgcc tgcctcaaaa acaccattat tggtaattgt tccggtaatc ctgatggtgt
     7861 tttccgttac tgtaagggag gagccgtttt caattgtcag attctttatg gctccggcag
     7921 caccggtgct cagaaccggt ttgttagtta cattccctat caggacggaa taagcagcag
     7981 aaggaacaac accgcacgac cagttggccg gattgttcca gtcactgtct gccgagcctg
     8041 accagacaaa ttcagatata attgatattg tacttgttgc tgtgactgcg ggacatcctc
     8101 ctgtggccgc tatagtgttt gttacaatgt agtcacccgg ggtgctggca gacaggtcga
     8161 cctgaccggt tgaatgatct gtaaatacaa ggccaggcgt cgagctgaaa taaccgggta
     8221 ctcctccgcc tgtgaaaacg ggaaagggat ttgttcccat cggacagtac ggattgccgg
     8281 gataaatgaa tgttgctacg ggcggaattg ttacaacaat atcaacagac ctgggaatac
     8341 ttgctccgca actgttcacc gcactgacac tcagtctgcc ggaagtggcc gccggagaaa
     8401 aatccagtgt tacactgttc ccgttcccgg aaatcgtaac tcctgagccc atggtgtaag
     8461 tccagttata tgaagttgcc gcgggatcgt ggggcactgt aaaaactact gctgactgac
     8521 cccggcagac tgtgatgctg ctttcagtaa agaagcccgg ttgcccggga atgctgttcc
     8581 aggtgatcag cctctcgtca gtggaattag aacagccatt ggctcctgtg agtgtaagtg
     8641 ttgcagtagt tgaacctgat ggtgttgagg gggtgaatgt tgcaagtgca gggtcaggat
     8701 tctgtgtcca cgtgccacct gcaccgctcc acacgggagt tccgctgtat gagcctgatg
     8761 cagaggctcc ggtcattgca atcggcattg tgccagtgca gagtccgctt atatccggtc
     8821 cggcgtccgc tttgggagcc gggaagacag tgacggttct gctggcggtg ccagtgcagc
     8881 ctctgttgtc agtaactgtg cagatgagat caaattgtcc ccc
//
LOCUS       DAOSPX010000107         8884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7499_length_8884_cov_5.409446, whole genome shotgun sequence.
ACCESSION   DAOSPX010000107 DAOSPX010000000
VERSION     DAOSPX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7499_length_8884_cov_5.409446"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PLZ75_06745"
     CDS             <1..1000
                     /locus_tag="PLZ75_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOS72134.1"
                     /translation="FIKKYNPTTTLLAAYNYQKMPFYTRKMANATIGYNWNSNPYTTH
                     IVNPLQLNIVMLDTIDPAFREKIESSSYLAYSYRDVTILGSGYSLVFSNQTIKKSRDF
                     WFFRFNAETSGNLLEFISTLAGQEKKDGSYNVFGRPFAQYIRADLDLRYNLIINDVSS
                     VVYRGFIGAAIPYGNSQAIPFEKQYFGGGANGIRAWQVRTLGPGSHVPDRSSFLNQTA
                     DIKLETNIEYRFKLFWILESAFFVDAGNIWSYRDNSIEGAQFHFRNVMKDMAVGTGIG
                     LRFNLDFVLIRADAGMKLRDPSLPAGSKWISNWKDYSDKTEGGKIFTFVLGIGYPF"
     gene            1182..1823
                     /locus_tag="PLZ75_06750"
     CDS             1182..1823
                     /locus_tag="PLZ75_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOS72135.1"
                     /translation="MARFSPEALKDWFGYTRRERRSSFFLLLLIVSVAGIRFMVPGVR
                     MRLELSALEKDIAADSSVRIIVQENKTRQPGSARAETVIDLNRCDSASLMALPGIGPV
                     LSARIIRYRNLLGGYAEVSQLKEVYGLPEETYRSISGRLRADGKDVRKIIVNRADYRQ
                     LLRFPYFERYDVTAILKYRELQGRIKSCEELVENNILSREKAERVKWYLDFSE"
     gene            2040..2231
                     /gene="rpsU"
                     /locus_tag="PLZ75_06755"
     CDS             2040..2231
                     /gene="rpsU"
                     /locus_tag="PLZ75_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOS72136.1"
                     /translation="MIIVPVKEGENIERALKKFKRKFERTGVIRELRARQAFIKPSIR
                     KREQLKKAKYVQKMQESED"
     gene            2285..3172
                     /locus_tag="PLZ75_06760"
     CDS             2285..3172
                     /locus_tag="PLZ75_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOS72137.1"
                     /translation="MDHKESFLQYLRFEKRYSPHTLRSYRNDLNQFGAYLSDRFPGIE
                     SPEISSHHIRGWIVSLMEKGMDPASVHRKISCLRVFFRWQQREGLVAGNPADGIALPK
                     RKKNLPVFVAEEAMDDLLDKDFFSDDFTGLRNRTIIEMIYLTGIRRAELTGLNVGDID
                     FQDSSIRVTGKRNKQRIIPLTAPFVRRLREYLALRSEISSIQKETHLFLTEKGNKLYD
                     KSVYNIVRDYLNMVTTSEKKSPHVLRHTFATHMLNRGADLNAIKEFLGHASLSATQVY
                     THNTFGKLRKIYRQAHPRA"
     gene            3206..3505
                     /gene="raiA"
                     /locus_tag="PLZ75_06765"
     CDS             3206..3505
                     /gene="raiA"
                     /locus_tag="PLZ75_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOS72138.1"
                     /translation="MNVQIHSVRFDADKKLIDFVNQKLGKLTQYGEDIVNAEVYLRLD
                     KDQTKENKISEIKLDISGGPLFAKKQSKTFEEATDGAVNALKKQITKHKQKKRGV"
     gene            3620..3693
                     /locus_tag="PLZ75_06770"
     tRNA            3620..3693
                     /locus_tag="PLZ75_06770"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3654..3656,aa:Thr,seq:tgt)
     gene            3744..3826
                     /locus_tag="PLZ75_06775"
     tRNA            3744..3826
                     /locus_tag="PLZ75_06775"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3778..3780,aa:Tyr,seq:gta)
     gene            3901..3973
                     /locus_tag="PLZ75_06780"
     tRNA            3901..3973
                     /locus_tag="PLZ75_06780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3934..3936,aa:Gly,seq:tcc)
     gene            3986..4057
                     /locus_tag="PLZ75_06785"
     tRNA            3986..4057
                     /locus_tag="PLZ75_06785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4018..4020,aa:Thr,seq:ggt)
     gene            4121..5308
                     /gene="tuf"
                     /locus_tag="PLZ75_06790"
     CDS             4121..5308
                     /gene="tuf"
                     /locus_tag="PLZ75_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOS72139.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSEL
                     RSFDSIDNAPEEKERGITINTSHVEYQTANRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVNVPRLVVFMNKCDMVDDPEMLDLVEMEMRDLLGA
                     YQFDADNTPFIRGSALGALNGVAEWEDKVMELMDAVDTWIELPPRAIDKPFLMPVEDV
                     FSITGRGTVATGRIETGVVKVGDEIQIIGLGAEGRKSVVTGVEMFRKLLDTGEAGDNV
                     GLLLRGIDKDEIKRGMVITHPGAVKPHSEFKASVYILKKEEGGRHTPFGNKYRPQFYI
                     RTLDVTGEVTLPEGREMVMPGDNLEITVKLIYPVACSEGLRFAIREGGRTVGSGQITA
                     ILD"
     gene            5359..5434
                     /locus_tag="PLZ75_06795"
     tRNA            5359..5434
                     /locus_tag="PLZ75_06795"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5392..5394,aa:Trp,seq:cca)
     gene            5447..5638
                     /gene="secE"
                     /locus_tag="PLZ75_06800"
     CDS             5447..5638
                     /gene="secE"
                     /locus_tag="PLZ75_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOS72140.1"
                     /translation="MKIKGYFRESFTELVHKVTWPTWSELQNSAAIVMVAALIFALIV
                     ALMDAAFSRIMGFIYSLLY"
     gene            5653..6201
                     /gene="nusG"
                     /locus_tag="PLZ75_06805"
     CDS             5653..6201
                     /gene="nusG"
                     /locus_tag="PLZ75_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOS72141.1"
                     /translation="MDENVKKWYVLRAIGGKEKKVKEYIESEIAHHKLEDFVTQVLIP
                     TEKIYQIRAGKKISKERTFFPGYVLVEAVLVGEVPHILRNVPNVLGFLGSQDGEPVPL
                     RKSEVNRILGKVDELNASDEELGVPFFVGEAVKVIDGPFNSFTGIIEEVNEEKKKLKV
                     MVKIFGRKTPLELSFMQVEKEN"
     gene            6285..6731
                     /gene="rplK"
                     /locus_tag="PLZ75_06810"
     CDS             6285..6731
                     /gene="rplK"
                     /locus_tag="PLZ75_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOS72142.1"
                     /translation="MAKTVAGIIKLQIRGGAANPSPPVGPALGSKGVNIMDFCKQFNA
                     RTQDKAGKVIPVVITVYTDKSFDFVTKTPPVAVQLLETARSKKGSKEPNRERVAQISW
                     DQVRAIAEEKMEDLNCFTIEAAMKMVAGTARSMGIAVKGEAPVKIN"
     gene            6738..7433
                     /gene="rplA"
                     /locus_tag="PLZ75_06815"
     CDS             6738..7433
                     /gene="rplA"
                     /locus_tag="PLZ75_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOS72143.1"
                     /translation="MTKLTKNRKLVLDKIDRDRLYTLKEASSLVKEITTTRFDASVDL
                     DVRLGIDPRKSNQMVRGVVSLPHGTGKEIKVLVLCTPDKESEATEAGADYVGLDDYIE
                     KIKGGWTDVDVIITMPSVMGKVGQLGRILGPRGLMPNPKSGTVTMEIGKAVREVKQGK
                     IDFKVDKTGIIHASIGKVSFEPQKIAENAREFISMVNKLKPAAAKGTYIRSLYLSSTM
                     SPGIKIDPKGLDE"
     gene            7462..7983
                     /gene="rplJ"
                     /locus_tag="PLZ75_06820"
     CDS             7462..7983
                     /gene="rplJ"
                     /locus_tag="PLZ75_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOS72144.1"
                     /translation="MRREEKIAIVDSLTERIKEYSHFYLTDTAELNAADTTDLRRKCY
                     ENDIKLVVVKNTLLKRAFEQSGIDFQELYPVLKGTTSIMFANTGNSPAKLIRDFRRQH
                     DKPLLKGAYVQESFYIGDNMLDTLVTIKSREEMIADVILMLKSPALNVISALQSGANK
                     IHGVLETLSKKEE"
     gene            8017..8394
                     /gene="rplL"
                     /locus_tag="PLZ75_06825"
     CDS             8017..8394
                     /gene="rplL"
                     /locus_tag="PLZ75_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOS72145.1"
                     /translation="MADLKKFAEELVNLSVKEVNELAKILKEEYGIEPAAAAVAVAGP
                     AASAEAPAAAEKTSFDVILKSPGGQKLQIVKLVREITSLGLKEAKALVDAAPGPVMEG
                     VSKEEAESVKNKLVDAGAEVELK"
     gene            8492..>8884
                     /gene="rpoB"
                     /locus_tag="PLZ75_06830"
     CDS             8492..>8884
                     /gene="rpoB"
                     /locus_tag="PLZ75_06830"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841948.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOS72146.1"
                     /translation="MSSKNTERICFSSREGQFEYPDFLGVQLKSFGEFFQLGTTPESR
                     KKERLYKVFTENFPITDTRNNFVLEFLDYYIDPPRYTIEECIERGLTYSVPLKAKLKL
                     YCTDPEHEDFDTVIQDVYLGTVPYMTERG"
BASE COUNT         2706 a         1763 c         2135 g         2280 t
ORIGIN      
        1 ctttatcaag aagtataatc ccaccactac tctcctggct gcatacaatt accagaaaat
       61 gccgttttat acgaggaaaa tggccaatgc cacaatcggc tacaactgga acagcaatcc
      121 ctatacaact catattgtaa atccccttca gcttaatata gtaatgctcg ataccattga
      181 tccggcattc agggaaaaaa tagagtcatc ctcatacctg gcctacagtt accgggatgt
      241 caccatcctg ggaagcggct attcccttgt attcagtaat cagaccataa aaaaatcaag
      301 ggatttctgg tttttcaggt ttaatgcgga aacttccggc aatctgctgg agtttatcag
      361 tactctggcc ggacaggaaa agaaggacgg aagctataat gtattcggcc gtcctttcgc
      421 ccaatacatc agggcagacc ttgacctgag atacaatctg ataataaatg atgtaagttc
      481 ggttgtgtac aggggattca tcggagccgc aatcccgtac ggaaattcac aggcaattcc
      541 tttcgaaaag cagtatttcg gaggaggggc caacggtatc cgggcctggc aggtaagaac
      601 tctcggtccg ggttcccatg tgcccgaccg cagcagcttc ctcaaccaga cagccgacat
      661 caagctcgag acaaatattg aataccgttt caagctgttc tggatactgg aaagtgcctt
      721 tttcgtggat gcaggaaata tctggtccta cagggataat tccattgaag gggcacagtt
      781 tcatttcagg aatgtgatga aggacatggc cgtgggaaca gggatcggat tgagattcaa
      841 tcttgacttt gtgcttataa gggctgatgc ggggatgaaa ctgagggatc cttccctgcc
      901 ggcgggttca aaatggataa gtaactggaa ggattattct gacaaaaccg aaggcggcaa
      961 aatcttcacc tttgttctcg gaataggcta tcctttctga gaaagggaat ccggccgccg
     1021 ccgggcgcgc catgcaggga aaggcatcat cagctgaggc tgtgctgctg caaaatgatc
     1081 cgtttcagaa agtctttttc agaaaagagc aggatacatc aggctattct tttatttatt
     1141 gcggtttatt cataaattgc agcaggtctt actgcattta tatggctcgt ttttcaccgg
     1201 aagctttgaa ggactggttc ggatataccc gccgtgaaag aagatcatcc ttttttcttc
     1261 ttctgttgat tgtttcagtg gcgggtatca gattcatggt gcccggtgtc agaatgaggc
     1321 tcgaactgtc agctcttgaa aaggatattg ccgcagacag cagtgtcagg atcattgttc
     1381 aggagaataa aacccggcag cccggatctg ccagggccga aacagtcata gatctcaaca
     1441 gatgtgattc agcatcactt atggctttgc ccggaatcgg tcccgtactt tctgcccgca
     1501 tcatcaggta caggaacctc ctgggcgggt atgccgaggt gagtcagctg aaggaggtat
     1561 acgggctgcc tgaagaaacc tacaggagta tttccggcag actgagggct gacgggaagg
     1621 atgttaggaa aataattgtg aacagggctg attacaggca gttattgcgc ttcccctatt
     1681 ttgaaaggta tgatgtgact gcaatcctga aataccggga actgcaggga agaatcaaga
     1741 gctgcgagga gctggttgaa aataatattc tcagcaggga aaaggctgag agggtaaaat
     1801 ggtacctgga tttcagtgaa taaattccct ccggttttaa gtaaacttta acgtttgcag
     1861 gctttgattt tggcaaaata aagcctatct ttagtttcta tgagtcaatg atgaatatta
     1921 accaggcaaa taactatatc ttatgaaatt acgtcatatg aataaattat ctccataaat
     1981 gttttgaaaa gaatgattca atttatacat ttgcgcctcg ttttaacaca gaatatttta
     2041 tgattatcgt accagtaaaa gaaggcgaaa atatagagag agctttaaag aagttcaaac
     2101 gtaagtttga aaggacggga gtgatacgcg aattaagggc ccgtcaggca tttattaagc
     2161 cttcaatccg caaaagagaa cagctaaaga aggctaaata cgttcagaag atgcaggaaa
     2221 gcgaagattg attccctttc cgcttcggaa tagttttttc tttacaccca cataaggttc
     2281 ctttatggat cataaggaat ccttcctgca atatttgaga tttgagaagc ggtattcgcc
     2341 gcatacatta aggtcgtaca ggaatgatct caatcagttc ggggcatacc tttcggaccg
     2401 tttccccggt attgaatccc cagaaatatc ttcacatcac atccggggat ggatagtgag
     2461 cctgatggag aagggaatgg atccggcaag cgttcacagg aagatttcat gcctgagagt
     2521 gtttttcagg tggcagcaga gggaaggcct ggttgccgga aatccggcag acggtatagc
     2581 gcttccgaaa agaaagaaga acctgcctgt cttcgtggca gaggaagcta tggatgattt
     2641 gctggacaag gatttcttca gtgatgattt taccggactg aggaaccgta cgatcattga
     2701 gatgatatat cttaccggga tccgcagggc ggaactgaca ggcctgaatg tgggtgatat
     2761 tgattttcag gactcaagca tcagggtcac cggaaaaaga aataagcaga gaatcattcc
     2821 ccttaccgca ccatttgtca gaaggctcag ggaatatctc gcgctgcgaa gcgaaatcag
     2881 cagcattcag aaggaaaccc atttgttcct tactgaaaag ggaaacaaac tatatgataa
     2941 aagcgtttac aatattgtca gggattatct gaatatggtt accacaagtg aaaagaagag
     3001 tcctcatgtt ttaaggcata ctttcgccac acacatgctt aacagggggg ccgatctcaa
     3061 tgccataaag gaattcctcg gtcatgccag cctttcggca acacaggttt atacacacaa
     3121 tacatttggt aaactcagga aaatatacag acaggctcat ccaagagctt aatatttata
     3181 attaataaaa acaggaggtg ctactatgaa cgttcagatt cattcagtac ggtttgatgc
     3241 cgacaaaaaa ttaatagatt ttgttaatca gaaactggga aaactgactc agtacggaga
     3301 ggatattgtg aatgctgaag tctatctgag actggacaag gatcagacaa aggaaaataa
     3361 aattagcgaa atcaaactgg atatttcggg tggaccttta tttgccaaaa agcaaagcaa
     3421 aacttttgaa gaagctacag acggggctgt aaatgccctg aaaaaacaga ttaccaaaca
     3481 taagcaaaag aagaggggag tatagaaatt ccattattta aaataatctg atttattttg
     3541 ataaataaaa tttaattgca tacatttgca caccgttttt taagggtgat ttatgccctg
     3601 atggtgtgtt ctttaaattg ccgatgtagc tcagttggtc agagcagctg atttgtaatc
     3661 tgcaggtcgg gggttcgaat cccttcatcg gctcctgaaa gagttaaaag tgtatgtctc
     3721 aagagtttaa ttctgttttt tgaggggaga taccaaagcg gtcaactggg gcagactgta
     3781 aatctgctga cgaatgtctt cgtaggttcg aatcctgctc tccccacttc aaacagaata
     3841 atgttcttaa atcttcagat ccggatttaa gcattcataa aacttcatgg aggatattaa
     3901 gcgggagtag ctcagttggt agagcattag ccttccaagc taagggtcgc ggattcgagc
     3961 tccgtctccc gctcactttt ttgaggccag agtagctcag gggtagagca cttccttggt
     4021 aaggaagggg tcgggggttc aattcccccc tccggctcaa tgaaaacaaa agatgtaaaa
     4081 aaactgatat aataaactta aatttaataa gcttggagtt atggcaaaag aaaaatttga
     4141 caggtcgaaa ccgcatgtaa acatcggtac cattggtcac gttgaccacg gtaagaccac
     4201 tttaactgct gcaattacaa cagtacttgc aaaaaaagga ctttctgaat tacgttcatt
     4261 cgattctatc gacaacgctc cggaagaaaa agaacgtggt atcactatca acacttcgca
     4321 cgttgagtat caaacagcta atcgtcacta tgcacacgtt gactgtccgg gtcacgctga
     4381 ctatgttaaa aacatggtta ccggtgctgc tcaaatggac ggtgctatca tcgtagttgc
     4441 cgccaccgac ggtccgatgc cacaaactcg tgaacacatc ttattagctc gtcaggtaaa
     4501 tgtacctcgt ttggttgtgt ttatgaacaa atgcgatatg gttgatgacc ctgaaatgtt
     4561 ggatttggtt gaaatggaaa tgcgcgattt gctcggtgct tatcaatttg acgctgacaa
     4621 cactccgttt attcgcggtt ctgcccttgg cgcattgaac ggtgttgccg aatgggaaga
     4681 caaagtaatg gaattgatgg atgctgtgga cacttggatt gaattacctc cgcgtgcaat
     4741 agataaacca ttcttgatgc ctgttgaaga cgtattctct atcacaggtc gtggtacagt
     4801 tgctactggt cgtatcgaaa ccggtgttgt taaagttggt gatgaaattc agatcatcgg
     4861 cttgggtgcc gaaggcagaa aatcagttgt tacaggtgtt gaaatgttcc gcaaattgct
     4921 cgatacaggc gaagctggtg ataatgttgg tttgttgctc cgtggtattg acaaagacga
     4981 aattaaacgt ggaatggtta tcacgcaccc gggtgccgtt aaacctcact ctgaattcaa
     5041 agcttctgtt tatattttga agaaagaaga aggtggacgt cacactccgt ttggtaacaa
     5101 atatcgtcct caattttaca ttcgcacgtt ggatgttacc ggtgaagtta cgttgccgga
     5161 aggtcgtgaa atggtaatgc cgggcgacaa cttggaaatt actgtaaaat tgatttatcc
     5221 ggtagcttgc agcgaaggtc tccgttttgc tatccgcgaa ggtggacgta ctgtaggttc
     5281 aggacaaatc acagccattt tggactaata aaaattattt aacaaaataa taatagtcgg
     5341 cgtaatgccg actattttac gggtgtagct cagttggtag agcactggtc tccaaaacca
     5401 ggtgtcggga gttcgagtct ctcctcccgt gccaaatttc agtataatga agataaaagg
     5461 atattttcgg gaatcattca ctgaacttgt gcataaggta acatggccga catggagtga
     5521 acttcagaac agtgcagcca ttgttatggt cgctgctctg atattcgcac tgatcgtggc
     5581 tctgatggat gcggcattca gcaggataat gggatttatt tacagtttgt tatattaatg
     5641 aagagctccc agatggatga gaatgttaag aagtggtatg tgctcagggc aatcggcggg
     5701 aaggaaaaga aggtaaaaga gtacattgag agtgaaattg cacatcataa acttgaagat
     5761 ttcgtcacac aggttcttat cccgacagaa aagatttacc agataagagc cggtaagaaa
     5821 atcagcaagg aaagaacctt ttttcccggc tatgtgctgg ttgaagccgt ccttgtcggg
     5881 gaagtgcccc atatcctcag gaatgtcccc aacgtgctcg gatttctggg atcacaggac
     5941 ggagaacctg ttcccctgag gaaatccgag gtaaacagga ttctgggcaa ggtggatgaa
     6001 ctgaacgcca gcgacgagga actcggcgtc ccgttctttg tcggggaagc tgttaaggtt
     6061 atagacggcc ctttcaacag ctttacggga attattgaag aagtaaatga ggaaaagaaa
     6121 aagctcaagg taatggtaaa aattttcggc aggaaaactc cgcttgagct tagttttatg
     6181 caggtagaaa aggaaaactg aaagaagata ttgatggctg taataattat aatgcttcct
     6241 gaatagctgt catactctcg caataaatac gcaaatttta aattatggca aaaacagttg
     6301 ctggaattat caagttacag attcgtggcg gggctgccaa tccatcaccg cctgtaggac
     6361 ctgcgctggg ttcaaagggc gttaacataa tggatttctg caagcagttc aatgccagga
     6421 ctcaggataa agcgggaaaa gttataccgg ttgtgatcac cgtttatacc gacaagtcat
     6481 ttgactttgt cacaaaaaca ccaccggtag ctgtccagct tcttgaaaca gccagatcga
     6541 agaaaggctc aaaagagcct aacagagaga gggtggcaca gatttcctgg gaccaggtca
     6601 gggcaattgc agaggagaaa atggaagacc tgaattgttt tacaattgag gcagccatga
     6661 agatggtggc cggtaccgcg agaagtatgg ggatagccgt aaagggtgaa gccccggtaa
     6721 aaattaatta aagagaaatg accaaactta ccaaaaaccg aaagcttgtt cttgataaga
     6781 tcgacaggga caggctctat acgttgaaag aagcatcctc actggtaaag gaaataacaa
     6841 ccaccaggtt tgatgcttcc gtcgacctgg atgtaaggtt aggtattgat ccgagaaagt
     6901 ccaaccagat ggtgcgcggc gttgtatcac tgcctcatgg aacgggcaag gagataaaag
     6961 tcctggtgct ttgcactcct gacaaggaaa gcgaagccac cgaagccgga gccgactatg
     7021 ttggtcttga cgattatatc gaaaagatca agggaggatg gacagatgtg gatgtgataa
     7081 taaccatgcc gtccgtgatg ggaaaagtgg gtcagctggg aagaatactc ggtccgcgcg
     7141 gactgatgcc caatcccaaa agtggaactg ttaccatgga aattggtaaa gctgtcaggg
     7201 aagtgaaaca gggcaagatt gatttcaagg ttgacaaaac cggaatcatt catgcttcaa
     7261 taggaaaagt ctcatttgag ccccagaaga tcgccgagaa tgccagggag ttcatctcta
     7321 tggtaaacaa gctcaagccg gctgcagcaa agggaaccta catacgtagc ctctaccttt
     7381 caagcactat gagcccggga ataaaaatcg atcccaaagg acttgatgaa taatgtatta
     7441 tgttaaaact cagatgctaa tatgagacgg gaagaaaaaa tcgctattgt tgacagcctc
     7501 actgagagaa taaaggagta cagccacttt tatctgactg ataccgctga actgaatgca
     7561 gcagacacaa ctgacctgag aagaaagtgc tatgaaaatg acataaagct tgtcgtggta
     7621 aaaaacactc ttctcaaaag agcatttgaa cagtctggca ttgactttca ggaactgtat
     7681 cctgtcctga aaggaactac gtccatcatg tttgccaata caggaaacag ccctgcaaaa
     7741 cttatcaggg atttccgcag gcagcatgat aaacctttgc ttaagggagc ttatgttcag
     7801 gaatcatttt atatcggtga caatatgctt gatactctcg tcacaatcaa atcaagagag
     7861 gaaatgatag ctgatgtgat actgatgctt aagtcacctg cactgaacgt gatctctgcc
     7921 ctgcaatcag gtgcaaataa aattcacggt gttcttgaaa cactttcaaa aaaagaagaa
     7981 taatcaatgt ataatcaatt taaatcttta taaaaaatgg cagatcttaa gaaatttgca
     8041 gaagaacttg ttaacctgtc cgtgaaagaa gtaaacgagc tggctaaaat actgaaggaa
     8101 gagtatggta tagaaccggc tgccgcagcc gtagccgttg ccggccctgc tgccagtgcg
     8161 gaagcacccg ctgctgctga aaaaacatct ttcgatgtca tcctgaaaag cccgggcgga
     8221 caaaaattac agattgtcaa gcttgtcagg gaaataacct ccctcggact caaggaagcc
     8281 aaggcactcg ttgatgcagc acccggcccc gtcatggaag gtgtgtcaaa agaagaagca
     8341 gaatcggtta aaaacaaatt ggttgacgca ggagctgaag ttgaacttaa ataaaatcat
     8401 agctcataac cgggttacag gtttagttcc gccatatggc agaactaaac ctttcgttct
     8461 ttaaattata tgtttattta atcttgttaa aatgtcttca aaaaataccg aaagaatatg
     8521 tttttcatcc agggaaggcc agtttgaata tccggatttt ctgggagttc agctgaaatc
     8581 cttcggtgag ttctttcagc tcggaacaac tcccgaaagc aggaaaaagg aaagacttta
     8641 caaagtgttc accgaaaatt ttccgataac cgacacaagg aataatttcg tcctggaatt
     8701 tttagattat tacatcgatc cgccacggta tacaattgaa gaatgtattg aacgcggact
     8761 gacatacagt gtgccgctca aggcaaaact caagctgtac tgcactgacc ccgaacatga
     8821 agattttgac acggtaatcc aggatgttta tcttggaaca gttccctata tgaccgagcg
     8881 cgga
//
LOCUS       DAOSPX010000108         8883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7502_length_8883_cov_4.169687, whole genome shotgun sequence.
ACCESSION   DAOSPX010000108 DAOSPX010000000
VERSION     DAOSPX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7502_length_8883_cov_4.169687"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..757
                     /locus_tag="PLZ75_06835"
     CDS             377..757
                     /locus_tag="PLZ75_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72147.1"
                     /translation="MKQLISLILLTLLSFSAINAQTSKVSKDPAGEWKYEAPYAPSEY
                     SAGVIKISFADKKYSAVITLPWSDYKINGENVKYENDSLTFSVYIEGESISIVMKMDD
                     ASKMTGKALYSMGEIPLTAKRVEK"
     gene            857..2470
                     /locus_tag="PLZ75_06840"
     CDS             857..2470
                     /locus_tag="PLZ75_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOS72148.1"
                     /translation="MNLSLKLIDWLVLGVVLGGSLGFGMYMAYRKKSGSSSTNFFLGG
                     RSIKWPVIGASLFATNIGAEHLVGLSGDAYRYGLSAGLVELTTVITLGFSCAFLFPCY
                     MKNKLFTIPEFLELRYNRRARTFFSGLMLVISIMTKLAFTLFAGALVLNSILGWNIMP
                     TIFYLGLTVAVFTIIGGFTAVAYTDSIQVIIMISGAAIMTVMGLNRAGGFGGLMEKVP
                     EMMSVARPYDDAVYPFWGILATAFYAGIFYWGIDQVNVQRVLAAPDLNNARKGAMFAT
                     LLKLFPIFIFALPGVIAFALFPGELQGDETKQTFVLLLDRLLPAGLRGLLMASLLAAL
                     ITSLIGVLNSVSTLFVRDFIVEFRPAFPEKKQVALGRLVILVVTLLGIGAAYLVYKNE
                     EGLYKYLQTITAYLVIPVFPAIFFGITSKKVTLKGAAVSVIAGLVLATLFVTDQLLGP
                     EAGSRLFPFLYHKLTLNFGYRGLWAELLITAVLFIVSAFTEKTDPLKLKTTTVDYSGG
                     IAPFEGLKDWRLHLFLLTVITILILLWLR"
     gene            complement(2473..3579)
                     /locus_tag="PLZ75_06845"
     CDS             complement(2473..3579)
                     /locus_tag="PLZ75_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72149.1"
                     /translation="MTFRKFDRDLLHIRKLSERRNLIMIERAAVPVSALPMSLSRENR
                     ELIRKTADRIRKAREENSSVILAFGAHTIKNGLAPILISLIEKGWITHLATNGAGIIH
                     DWEFAFQGMSSEDVRENVKNGQFGIWEETGKYINLALVAGAYEGHGYGESVGKMISRD
                     GIDIPDPDQLLNEAVINSRTDPSRAAAALDFYHLIEKSGIRSGFLGIPHPFRKYSVQC
                     AAFDLKVPFTGHPMIGHDIIYNHPLNSGSAIGRTALTDFLYFAGSVNNLENGVYLSVG
                     SAIMSPMIFEKALSMAQNLKIQNNSHIDNHFMLVVDLAKAEWDWSRKGEPPHDNPAYY
                     LRYCKTFSRMGGEMHYMTADNRDFLLELNHELRT"
     gene            complement(3576..5480)
                     /locus_tag="PLZ75_06850"
     CDS             complement(3576..5480)
                     /locus_tag="PLZ75_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS72150.1"
                     /translation="MDKELLEEVINRIKDASIAIVGDFCLDAYWFTDDSKSEISIETG
                     EPTIPVKEQKYSLGGAGNVASNLAAMQVKDVRAFGVIGNDPFGVEMVRLMKQAGISTD
                     NLLIQNQNWSTHVYIKPYSGDIEKSRIDFGNFNVLSVETADLLLSSLKDQVAETDLII
                     INEQVLSGIHTAYFRKKLAELINLFPQTIFIADSRNHIGDYRGAWRKMNEVEASRQCS
                     IDRNPDEIVPLAEARNSAATLFERYGKPLLVTRGSRGSLIADENGIKEIPGLMILSRV
                     DTVGAGDSYLAGAASALAAGYSAEIAAELGTLVAGVTVRKLFQTGTASPEEILQAGTD
                     TDFTWRPDLAGDIRQAKYHGNTEVEIINKWPGKPVIRHAVFDNDGTVSTLREGWEKLM
                     APVMIRAILGNKYSGADEALLKKTEARVYQFIDSTTGIQTLMQMKMLLGIIRESGFVS
                     EKEMLDEYGYKEIYNRELLAMVKKREDKLLAGELNVEDLTIKNAIPFLKILYDSGIKL
                     YLASGTDTEDVRHEAEVLGYDYLFEGRIYGAVGDINKEAKKMVLDRILDSLDKSDPES
                     IVTFGDGPVEIRETQKRGGLTVGIASNELRRYGLNLSKRTRLIKAGADIIMPDFSQSE
                     YLFELLNIKL"
     gene            complement(5562..6230)
                     /locus_tag="PLZ75_06855"
     CDS             complement(5562..6230)
                     /locus_tag="PLZ75_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOS72151.1"
                     /translation="MKRKILLDNLIGKYPELSELRDAIDKASGIIVKCYTAGGKLLIC
                     GNGGSSSDACHIAGELMKSFEKRRPLDVSFKKRLADISPDRGRYLAEKLEHGLPAISL
                     AAHTSLITAISNDIGPRLIFAQQIIGYGEGKDVLLALSTSGNSENIIDACITARAMNI
                     TVVGITGKSGGKMKEYCDILINVPREQTASVQELQLPVYHAICRIVEEYFYGEETNNL
                     KDKD"
     gene            complement(6329..8347)
                     /locus_tag="PLZ75_06860"
     CDS             complement(6329..8347)
                     /locus_tag="PLZ75_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS72152.1"
                     /translation="MLTSKQFSRLLCFFVFFLSTVHITAQDPQGTILLPGTYEIDTIT
                     IRARIRLVDPVAKPYTETVSLFPTISTVRRSDIRNQGARTLIDAVSFIPGGFTETRGR
                     QVKQFFSVRGQKYPYPDYAINGVWQKEFEELPYFLSSSDIEEIEVIRSSAALLTGLSG
                     LAGFINIKTREYERFETTLDLEYGTFNSLHSHISTGNRIGNFSYAAGLGFDRTSGPEG
                     RHAKESMTDLYTRMNWKITDKLGLNASLFYLNGERQMAIALPPAEDRVRNMIQGFDPY
                     KAFLSNVKLTYRPVSALSSELQLFYTRRDPTFYDKVEETTASEKDYEFGLNFIQSVRI
                     LPDNVLRFGGLYNHWIAPYGKRFYMGKRCDTETFSGVLTDEQRLGPVTLDAGIRITRT
                     YLNDYGAFNIEGGGGPFRQVEPVKDEWEPAIVQGSFGITYNTTEKFSLFLNSAAGNIR
                     PRAGTLLDNNGGEPVNEPLNETRYKIDIGLLHNIGEDGKMTLTAFGVYQSNGIVLSGT
                     TYLDPQTNIRRELYHNRNQYQKGIEFEITAPLLFEIIKPFFNFTIMQSKMEENNKMVI
                     NKEHPVWISNAGIYSAWNNFDLNIMGKYVSPFENDRFVRPADGPQPLGDFLVLDCNGG
                     YTTKWNTPVRFYARIKNLTDRKYSTVNGYPDFGRMLFGGIQIKFMSKS"
     gene            8556..>8883
                     /locus_tag="PLZ75_06865"
     CDS             8556..>8883
                     /locus_tag="PLZ75_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72153.1"
                     /translation="MKLIAIFFSLFVTSLSGSGHYDWPMWRFDHGRSASTPEQLADKL
                     FLQWQVNYSPREPVWDDPLNQNLMQYDRIFEPIVAENKLFIGFNDQDKVSALDISSGM
                     ELWHFFT"
BASE COUNT         2196 a         2072 c         1921 g         2694 t
ORIGIN      
        1 tccctacaag aggacccaca tcattcatga tccttttctc tatgggatcg tacaacctgc
       61 cgcttgcgat ccagtcggca tatacaagct tctttaaccc gtaaggagtt tcaatttcct
      121 tgtcaatacc cgtaatatgc ctgcggaact gcaggaaata tttttccaga tctgtcataa
      181 aaatcccctt aaatagttta tgtaaaaata attattaatc cctcattatc aatatttata
      241 cttgtgtaag agattgtttc tgaaaaaaac tgccccggag ttgcaaatat atgatgaagg
      301 gtaaggtact catattcaaa atttgtttat tttgagatag cagattttta tcataattca
      361 aattaaattt caggctatga aacaactcat ttccttaatt ttgctgacat tactcagctt
      421 ttcggcaatc aatgcacaga cttcgaaagt aagcaaggac cctgcaggag aatggaaata
      481 tgaagctccg tatgctcctt cagaatattc cgccggagtg ataaagattt catttgcaga
      541 taaaaaatat tctgcagtaa ttactttacc ctggagtgac tataaaataa acggagagaa
      601 tgtaaaatat gaaaatgact ccctgacttt ttctgtttac atcgaggggg agtcaatttc
      661 cattgtgatg aagatggatg atgcatcaaa gatgacaggc aaagcattat attcaatggg
      721 agaaattccg ctgacggcaa aaagggtaga gaaataggac cgctttctga tatttgccgt
      781 ttccggcatc atcagaacag tgcggaactg attttaatgc tgactgatac cctgtcagga
      841 tcaataaaca ttatcgatga atctctcctt aaaactgatt gactggctgg tgctgggggt
      901 tgttctcggc ggaagtctcg gttttggaat gtatatggca tacaggaaaa agtcgggaag
      961 cagcagtaca aatttctttc ttggaggaag gtccatcaaa tggcctgtga tcggcgcatc
     1021 cctgtttgcg acaaacatag gagcagagca tcttgtagga ctttcaggcg atgcataccg
     1081 ctatggcctt tctgccggcc ttgtggaact gaccacagtg atcacactgg gtttcagttg
     1141 tgcttttctt ttcccctgtt acatgaagaa caagctgttc actattccgg agtttcttga
     1201 attaagatat aacagacggg ccaggacgtt cttttccggg ctgatgcttg taatcagcat
     1261 aatgacaaaa cttgctttca ctctttttgc aggcgccctt gttctgaaca gcattcttgg
     1321 ctggaatatc atgccgacca ttttttatct tggacttacc gttgcagtgt ttaccatcat
     1381 aggcgggttc acagccgttg cttataccga ttccattcag gttataatca tgatatccgg
     1441 tgcggcaatc atgacagtta tgggattaaa cagggctggc ggcttcggag gactgatgga
     1501 aaaggtaccg gaaatgatgt cggttgccag accttatgat gatgctgtat atcctttctg
     1561 ggggatactg gcaacggcat tttatgcggg tattttttac tggggcatcg atcaggttaa
     1621 tgtacagaga gtacttgcgg caccggatct taataatgcc agaaaaggtg caatgtttgc
     1681 caccttgctt aaactgtttc ccattttcat atttgcattg ccgggagtta tagcatttgc
     1741 tctgttcccc ggtgaacttc agggtgatga aacaaagcag acctttgttc tccttcttga
     1801 cagactgctt ccggccggtc tgaggggcct gctgatggct tctctccttg ccgcacttat
     1861 tacatccctg atcggagtgc tgaattcagt atccaccctg tttgtccggg attttattgt
     1921 tgaattcagg cctgcctttc ccgaaaagaa gcaggttgct ctgggaaggc ttgtcatact
     1981 cgtggtaacc cttcttggaa taggtgccgc ttatctggtg tataaaaacg aggaaggttt
     2041 gtataaatat cttcagacaa tcactgcata tcttgtcata cccgttttcc ctgcaatttt
     2101 tttcgggatt accagtaaaa aagtcacact taagggagct gccgtttcag tgattgcagg
     2161 ccttgtactt gccactcttt ttgtgactga tcagcttctc ggccctgagg ccggcagcag
     2221 attgtttcct tttctgtatc ataaactcac ccttaatttc ggttaccgtg gtttatgggc
     2281 tgaattgctg attaccgcag tgttatttat cgtttcggca ttcacggaaa agaccgatcc
     2341 ccttaagctt aagacaacaa ctgttgacta ttccggggga attgccccgt ttgaaggtct
     2401 gaaagactgg cgccttcacc tttttctgct gacggtcatt acaatactta tcctgttatg
     2461 gcttagatga tctcaggtcc tgagttcatg attcagttcc agcaggaagt ctctgttgtc
     2521 agctgtcatg taatgcatct caccgcccat tctgctgaac gtcttgcagt aacgcagata
     2581 gtaagcggga ttgtcatgag gaggttcacc tttccgcgac cagtcccatt cagcttttgc
     2641 aagatccacc accagcatga aatggttatc tatatggctg ttattctgga ttttcagatt
     2701 ctgtgccatg gaaagtgcct tctcaaaaat catcggggac attatggctg acccgactga
     2761 gagatacacc ccgttctcca gattatttac gctgccggca aaatacagga agtcggtcag
     2821 tgctgtgcgg cctatggcag aaccgctgtt caggggatgg ttataaatta tatcatgacc
     2881 aatcatcgga tgtccggtaa aaggcacctt cagatcaaat gcggcacatt gtaccgaata
     2941 cttcctgaac ggatgaggga taccaagaaa tccggaccgg atccctgatt tttcaattag
     3001 atgataaaaa tcaagggcag ctgctgccct cgaggggtca gtccgggaat ttatcacggc
     3061 ctcattcaga agctgatccg gatcaggtat gtcaataccg tcgcgtgata tcattttccc
     3121 gacagattca ccatagccat ggccctcgta tgcaccggcg accagcgcca ggttgatgta
     3181 tttgcctgtt tcctcccaga tgccgaactg cccgtttttt acattttccc ggacatcctc
     3241 acttgacatg ccctggaagg cgaattccca gtcgtgaata attccggcac cgtttgtggc
     3301 aagatgggta atccatcctt tttcaataag gctgataagt atgggagcca gtccgttttt
     3361 aatggtatgt gcaccgaaag caaggattac cgaactgttt tcttccctgg cctttctgat
     3421 cctgtccgcc gttttccgga taagctccct gttctcacgg ctgagcgaca tgggcagagc
     3481 agaaacagga actgctgcgc gttcaatcat gatcagattc ctgcgttccg ataacttcct
     3541 gatatggagc agatccctgt caaattttct gaaggtcata acttaatgtt taacaactca
     3601 aacaagtatt ctgattggga aaagtcaggc attatgatat cagctcctgc ctttatcagt
     3661 cttgtccttt ttgacagatt gagtccgtat cgccttaatt cgttactggc aatccctacc
     3721 gtaaggccgc ctcgcttttg tgtttcgcgt atctcaacgg ggccgtcgcc aaaagtgaca
     3781 atggactcgg gatctgattt gtcaagtgaa tcaagtatac ggtcgaggac catttttttt
     3841 gcctccttgt ttatatctcc gacagccccg tagattctgc cttcaaaaag ataatcatag
     3901 ccaaggacct cagcttcatg cctgacatcc tccgtatctg taccactggc caggtaaagc
     3961 ttgattccgc tgtcgtacag aattttaaga aagggtattg catttttgat tgtaagatct
     4021 tccacattaa gctcacccgc caacagttta tcctcacgct ttttaaccat ggcaagaagc
     4081 tccctgttat agatctcctt atagccgtat tcatcgagca tctctttctc actgacaaag
     4141 ccggattccc tgataattcc gagaagcatt ttcatctgca tcagggtctg aattcctgtt
     4201 gtactgtcaa taaactgata aacccttgct tctgtctttt tgagcagcgc ttcatctgca
     4261 ccggaatatt tatttccgag aattgccctg atcataacgg gtgccattaa tttttcccag
     4321 ccttcacgaa gtgtggatac tgtgccgtcg ttatcgaata ctgcatgcct gatcacaggt
     4381 tttccgggcc atttgttaat aatttcaact tctgtgttcc cgtggtattt tgcctgacgt
     4441 atatctccgg ccagatcagg acgccaggtg aagtcagtat cagtccctgc ctgaagtatt
     4501 tcctcaggag atgcagtgcc cgtctggaaa agctttctta cagtcacgcc tgcaaccaga
     4561 gttcccagtt cggcggcaat ttctgctgaa tatccggctg ccagtgcgga cgccgcacct
     4621 gcaagataac tgtctcccgc accaacggtg tccacccggg acagtatcat cagcccggga
     4681 atctctttta taccattctc atcggcaatc agggaccctc tgcttccccg tgttaccaga
     4741 agcggttttc cgtatctttc aaaaagcgtg gcagctgaat tccttgcttc agccagaggt
     4801 acaatttcat ccggattcct gtctatactg cactgacggg aagcctccac ctcattcatt
     4861 tttctccagg ctcccctgta atcgccgata tggttcctgc tgtcggctat gaatatagtc
     4921 tggggaaaca aattaatcag ttctgccagc tttttcctga aatatgcagt atggatcccg
     4981 gaaagaacct gttcattgat tataatgaga tctgtttcag ctacctgatc ctttagactg
     5041 cttagcagca ggtcggctgt ttcaaccgac agaacgttaa aattgccgaa atcaatccgg
     5101 cttttttcaa tgtcgccgga atagggtttt atataaacat gtgttgacca gttctgattc
     5161 tggattaaaa gattatctgt agaaatgcct gcctgtttca tcagtctgac catttccact
     5221 ccgaacggat cattgccgat aacaccgaaa gctcttacat cctttacctg catcgctgca
     5281 agattgctgg ctacatttcc cgccccgccc agcgaatatt tctgctcttt tacaggtatt
     5341 gtcggttctc cggtttctat cgagatttcg cttttcgaat catcagtgaa ccagtaggca
     5401 tcaaggcaga aatcgcctac aattgcaata cttgcatcct ttatcctgtt gattacttct
     5461 tccagtagtt ccttatccat atctgtttgt tttacctgat ttcctgtttt ccagactttt
     5521 ccggctcctc gcttccccgg gtctccggca ccacgtcccg gtcagtcctt gtctttcaag
     5581 ttgtttgttt cttccccata gaaatattcc tcaacaatcc tgcatatagc atgataaaca
     5641 gggagctgca gttcctgtac ggaagcagtc tgttcccgcg gaacattgat aaggatgtca
     5701 caatattcct tcattttgcc gcctgatttc cctgttatcc caacaaccgt gatgttcatg
     5761 gctctggcag ttatgcaggc atctattata ttttccgaat ttcctgaagt actcagggca
     5821 aggagtacgt cttttccctc accatagcca atgatttgct gggcaaagat cagtcgggga
     5881 ccgatatcgt tgctgattgc ggtaatgagt gatgtatgag ccgctaatga aattgccggc
     5941 agtccgtgtt caagtttctc agccaggtat ctgccccggt ccggggatat atctgcaagt
     6001 cttttcttga aactcacatc caggggtctc cgtttttcaa aacttttcat cagttcaccc
     6061 gcgatatggc aggcatcgga actgcttccc ccgtttccgc aaatcagcag cttgccgccg
     6121 gcagtgtaac atttgacaat gattccggag gccttgtcta ttgcatccct taattccgat
     6181 aattcaggat attttccgat cagattatcc agtaagattt tccttttcat taaatcagtt
     6241 atactgtcat aaaaataaca taattcacgg aaattcccct aaggacagcc gggatattac
     6301 ccggttacct gataccggta ttttttcttc agctttttga catgaatttt atctggattc
     6361 cgccgaacag cattctgcca aaatcgggat atccgttgac ggtggaatat tttctgtcag
     6421 tcagattttt tattcttgca tagaacctta ccggtgtatt ccattttgtg gtatagcccc
     6481 cgttgcagtc aagaacgaga aaatccccca gaggctgcgg gccatccgca ggcctgacaa
     6541 aacggtcatt ttcaaaaggc gaaacatatt tacccatgat gttgagatca aaattattcc
     6601 aggctgaata aattcctgca tttgaaatcc ataccggatg ttccttgtta ataaccattt
     6661 tgttgttctc ttccatcttt gactgcatta tcgtgaaatt gaagaacggt ttgatgattt
     6721 cgaacagcag gggtgcggtt atttcaaatt caattccttt ctgatactga ttgcggttgt
     6781 gatagagttc cctccggata tttgtttgcg gatccaggta tgtggttccg ctcaggacaa
     6841 taccgttact ctggtataca ccgaaggctg tcagggtcat tttcccgtct tctcctatat
     6901 tgtgcagcaa tcctatatca atcttgtatc gggtttcatt caggggctca ttaacaggtt
     6961 ctcctccatt gttgtccaga agtgtacctg ccctgggcct gatattcccg gcagcggaat
     7021 tgagaaaaag cgagaatttt tcagtggtat tgtaggtaat tccgaaactt ccctggacta
     7081 ttgccggttc ccattcatcc ttcacaggtt caacctgcct gaaaggccct cctccgcctt
     7141 caatgttgaa tgccccgtag tcgttaaggt aggtgcgggt gatccttatc ccggcatcca
     7201 gggtaaccgg ccccagccgc tgttcatccg ttaacacgcc ggaaaaggtt tccgtatcgc
     7261 accttttgcc catgtaaaac cttttcccgt agggagcaat ccagtgatta tacaatcctc
     7321 cgaatcttaa tacgttgtcg gggagtattc ttacagactg gatgaaattc agcccgaatt
     7381 cgtaatcctt ttcactggca gtggtttctt caaccttgtc ataaaaggtc ggatcacgcc
     7441 tggtgtaaaa cagctgaagt tcggaagaca atgccgaaac cggcctgtat gtcagtttaa
     7501 cattggacag gaaagccttg taggggtcaa agccctgaat catgttccgg actctgtctt
     7561 ccgccggagg cagtgcaatg gccatctgcc tttcgccatt gagataaaaa agacttgcat
     7621 tcagtcccag tttgtccgtt attttccagt tcattcttgt gtacaggtca gtcatggatt
     7681 cctttgcgtg cctgccttcc gggccgctgg tcctgtcgaa tcccagacct gctgcataac
     7741 tgaaattacc aatcctgttt cccgtggaaa tatgggaatg aagtgaattg aaagtgccgt
     7801 attccaaatc gagggttgtt tcaaatcttt cgtattcacg ggttttgata ttgataaatc
     7861 ctgccagtcc tgacaaaccc gtcagtagtg cagcactcga tctgatcact tcaatttcct
     7921 ctatgtcgga tgaggacagg aaatatggca gttcttcaaa ttccttctgc catactccgt
     7981 taatcgcata atcaggatag ggatatttct gacctcttac ggaaaaaaac tgttttacct
     8041 gccttcccct ggtttcggtg aaaccacccg gaataaaact gacagcgtca atcagggttc
     8101 ttgcaccctg gttgcgtatg tcagaccggc gcacggtact gattgtcggg aaaagggata
     8161 cagtttcagt atagggtttt gccacggggt caacaagcct tatcctggcc cgtatggtaa
     8221 tggtatcaat ctcataggta ccgggcagta aaatagtgcc ttgcggatcc tgggcagtga
     8281 tatgaactgt tgaaagaaag aagacaaaaa aacataagag ccttgaaaac tgttttgaag
     8341 tgagcataat tgtaatttaa attgatattc tgatcagtgg cgcaaattaa aaaaatgaat
     8401 cttttaactt ggttaaaatt agttaaatca tttgtctctg ataatcattt tcagcactat
     8461 cagttcctta ttgtaaaaag gatgattatt tttaccttat ctgaaattta ttgtttgagt
     8521 tgaaacaaat gatgttttaa taccggaaat tcagcatgaa actgattgca atcttttttt
     8581 ccctgtttgt tacctcgttg tcaggttcag ggcattatga ctggccgatg tggagatttg
     8641 accacggccg ttctgcttcc actcctgaac agctggccga taagctgttc cttcagtggc
     8701 aggtaaacta ttctcccagg gaacccgtat gggatgatcc gctgaatcag aatctgatgc
     8761 agtatgacag gatctttgag ccgattgtgg cagagaataa actgttcatc ggtttcaatg
     8821 accaggacaa ggtatcggcc ctggatatca gttcagggat ggagttatgg cactttttta
     8881 ctg
//
LOCUS       DAOSPX010000109         8833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7557_length_8833_cov_4.119048, whole genome shotgun sequence.
ACCESSION   DAOSPX010000109 DAOSPX010000000
VERSION     DAOSPX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7557_length_8833_cov_4.119048"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLZ75_06870"
     CDS             <1..998
                     /locus_tag="PLZ75_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS72154.1"
                     /translation="IDGVDPYIVKGDPSSGLLPFIEKDPPAADGTGDRRIQAYCYRMT
                     LTDHPDNRIPFIKPEGYNETEYELLFRNYEAARGPVEEMYSYGDPLVPWINTNMPDRK
                     TDTNNQKGFSTDFIGQNYDYPEASYEEREKIAERHLKYQQGLMWTLAYHPRMPEKVRN
                     IVSQWGLCRDEFTENNGWTPQLYIREARRMISDYVMNQKHCEGIEVAEDPVGLAAYGM
                     DSHMVQRYVDGNGFVQNEGNVEAPVSGPYPVSYRSIIPRRDECSNLLVPVCLSASHIA
                     YGSIRMEPVFMILGQSAATAACIAVDERKNIQDINYQSLKTRLLEDGQVLSADAP"
     gene            1154..2236
                     /locus_tag="PLZ75_06875"
     CDS             1154..2236
                     /locus_tag="PLZ75_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOS72155.1"
                     /translation="MTSRRTFIKQAAAAGIAGTFPGMFLPRQQESSGKLIWAYLIHLS
                     YNMWEDNVPPKYRDEDYKCTSCQEAREWAHGFRPNLTFDYEVWDALLPEMAAAGINMV
                     VIDLGDGVEYESHPEIAVKNAWKPEKLRNELSRMRKLGIEPIPKLNFATTHDIWMKEY
                     SRMVSTDIYYAVCRDLIREVTELFDRPRFFHLGMDEELASYQTRFDYAVVRQNDLWWG
                     DLYFLIGEVEKNGVRPWVWSDYAWHKPDVFFRKMPRSVLQSNWYYYQDFDLNKMKEPL
                     KTYVKLYNDLEKHGYDQVPTGSNHSVNVNFERTVEYCKKVTDPSRLYGFMTAPWRPTL
                     IPCLGRHREAIAELSRAMKKFYPAFR"
     gene            2272..3852
                     /locus_tag="PLZ75_06880"
     CDS             2272..3852
                     /locus_tag="PLZ75_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS72156.1"
                     /translation="MNNNLFKALTLVTAGTAIAGCSSQQKSKQPNIVYIMSDDHAYQA
                     ISAYGYGLNETPNIDRLAKEGAIFTRACVTNSLSAPCRAVLLTGKHSFINGKVDNVTS
                     FNWDQDNFPKILQANGYQTAMIGKIHLDGIPQGFDFSMVLPGQGEYYNPDFLIEGERV
                     RKEGYVTDIITETALDWLKNKRDPDKPFCLLYHHKAPHRNWLPSPKYFNLYDDITFEP
                     PENFFDDYSGRGRAAREQEMEIDGHAMWGHDFKLIVGPDGDSTGFYRDLKRMNPEQRA
                     LWMEAYEKENEEFLKAKLIGKELAYWKFNRYIKDYLRTIKSIDDGVGELLDYLEEAGL
                     DENTIVIYASDQGFYLGEHGWFDKRFMYEESFRTPLLMRYPKEIKPGTVIDRLVQNLD
                     LAPTFLDYAGAKIPEDMQGVSFRNLVGGKTDEWRDAVYYTYYEYPEVHMVKRHYGVAT
                     ERYKLMHFYFDIDEWEMYDLQTDPAEMKNIYDDPAYAEVRTMLHNKLEELRKFYGDSD
                     ELNEKYLNQFLEKKNIKR"
     gene            3996..6170
                     /locus_tag="PLZ75_06885"
     CDS             3996..6170
                     /locus_tag="PLZ75_06885"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOS72157.1"
                     /translation="MIKNTTIFVLFLSLYVSILKGQTGDELNILKNNWLLYSDAANSL
                     YHHLTGEAYKLLDKRAETINQLRSAGDWQKRQEEVRRELWDIIGPFPEKTPLNARITG
                     KVRKKGYSIENVIYESLPGLHVTASLFIPDNIRKPAPAILFCCGHSHGAYRLEYYQLP
                     LLNLVKKGFIVLAFDPLSQGERLQYFDPGKGESLIGSSTKEHSYPAVQVFLNSKSIAR
                     YFIWDGIRGIDYLVSRREVDPQRIGVHGLSGGGTQTAYISALDDRVAASAPAGYITGF
                     RRLLQSIGVQDGEQNLYHQLTRGIDHADYIELRAPRPTLIMATTRDFFSIQGTRETYE
                     EVKRAYTMLGAPGNIEITEDDCGHDYTRKNREAMYAFFQKHLGLPGSSSEEEVDFATE
                     QELQKTSTGQLSTSLEGLTVFDLNKRESEPLLAGLEAKRKNYPEGLSDIVTSAGKLSG
                     FIYPSGGREPVFAGRFQRDDYAVEKYFVKGEGNYVIPYLLIKPRKPGNKAVICLYPGG
                     KPEMIQEGGEIEWFVRNGFTVLVPDIIGTGETGPGIFRGDAVIMGVSNNMWFTSVITG
                     KSIAGIRAADIIRLRMLLTQKEGIREVYGLAVKEMTPVMLHASAFDQEIAGTALVEPL
                     SSYRSIALNPFYHTGFVHNIVAGALTGYDLPDLAGTIAPRKLLMIGTTDGTGEGTGLE
                     NTETDLEVIRNCYRLKNASGHLSIIPDYTGDGLFEQLADWIK"
     gene            6385..7896
                     /locus_tag="PLZ75_06890"
     CDS             6385..7896
                     /locus_tag="PLZ75_06890"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOS72158.1"
                     /translation="MKKYLIWLLTTLRILVGWHFLYEGIIKLMTPGWTSKLYLMGSTW
                     LFSGIFQKMAESQMIVSIVDFLNIWGLILIGISLFIGLAVRWSSIAGAILLLFYFVAY
                     PPIPGHTFGTATEGSYLWVNKNLIELALLIVFAAVSSASFFGIDRLIKRWKEEKPHAP
                     VPPVKKESLTLQRRQMLRDLISIPFLGAFAYAVYRKRKWDSYERKFLAENTDALSGAT
                     LKTFTFSSLDDLKGQVPRGSIAGIELSRLIMGGNLIGGWSHARDLIYTDKLVKAYHTD
                     EKVMMTMQLAEKCGINAILTNPALCRIINKYWKETGGKMKFISDGGGSEESIRNSVNG
                     GACTVYVHGGDADRLVYNGKVDQIAKNLEIIRSYGMPAGIGAHRLETIIACVEAGIIP
                     DYWVKTIHHHNYWSAKVDLERQTTVDEGYKDNMWDFTPRETIEFMSRLEQPWIGYKIL
                     AAGAIRPEDGFRYAFSNGADFVCVGMYDFQVVEDCNIALDTLANVNRTRPWRG"
     gene            7884..8723
                     /locus_tag="PLZ75_06895"
     CDS             7884..8723
                     /locus_tag="PLZ75_06895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS72159.1"
                     /translation="MERIISLLAILSVLGGFPAQAQNIKTGSEKSASGFQWPEGKKMA
                     LSLSFDDARLTQIDKGIPLLDQYNVKATFYVSPKAMKQRAEGWRKAAANGHDIGNHSM
                     VHPCSGNFQWSRNRALEDYTLKKMRRELDSASLVIMEVTGVKPHSFGYPCGQKFVGRG
                     KKTKSYVPLIAAMFETGRGWLDEAPNDPAYCDMAKLYGMELDGKSFEEVLKIIETAKN
                     QGGWLIFAGHEMNDEQAHQTSLLSTLEALCRYATDPSNEIWIDNVHSIASYINKNRAG
                     NIK"
BASE COUNT         2594 a         1984 c         2164 g         2091 t
ORIGIN      
        1 ttattgatgg cgtggacccg tatattgtta aaggtgatcc ttcaagcggc ctgctgccct
       61 ttattgaaaa ggatcctcct gctgccgatg gtacgggtga cagacgtatt caggcctatt
      121 gctacagaat gacccttact gaccaccctg acaacagaat cccttttatc aaaccggagg
      181 gatacaatga aaccgagtat gagcttctgt tcagaaatta tgaagcagcc agaggacctg
      241 tcgaagaaat gtacagttac ggcgatccgc ttgttccgtg gataaacacc aatatgccgg
      301 accgcaagac cgacacaaac aaccagaaag gtttttcaac cgattttata ggacagaatt
      361 atgactaccc cgaagcgtcc tatgaggaac gcgaaaaaat tgctgaacgc catctaaaat
      421 atcagcaggg acttatgtgg acactggctt atcacccgcg gatgcctgag aaagtcagga
      481 atattgtttc acaatgggga ttgtgcaggg atgaattcac tgaaaacaac ggatggacac
      541 cccagctata tatacgtgaa gccaggcgta tgatcagtga ttatgtcatg aatcagaaac
      601 attgcgaagg aatagaggtg gcagaagatc ctgtcggact tgcagcatac ggtatggatt
      661 cccatatggt tcagcgatat gtggacggta atgggtttgt gcagaatgaa ggaaatgttg
      721 aagctccggt ctccggcccg tatccggtaa gctacagatc aatcataccg cggagggatg
      781 agtgcagcaa cctcctggtt cctgtttgcc tgagtgcatc ccacatagca tacggatcca
      841 taagaatgga acccgttttc atgatactcg gacagtcggc tgcaacggca gcatgcattg
      901 ctgtcgatga gagaaaaaat atccaggata tcaattacca atcactcaaa accagactgc
      961 tggaagacgg acaggtttta tctgcagatg caccctaaac atatctctcg tgatcggaga
     1021 aagtcaatgg ccggaaatgg aagacggaca ggttttacat gattttccgt gaaaatctgc
     1081 cggatttata aacctgattg tgccaactga aatttttcac gtacatttaa ttctgaaatc
     1141 aattcgtttt acaatgacca gcagaaggac attcatcaaa caggctgcgg ctgcaggaat
     1201 tgcaggcact tttcctggga tgtttctccc gcggcagcag gaatcatccg gaaaactcat
     1261 ttgggcatac cttatccacc tgagttataa catgtgggaa gacaatgtcc caccgaaata
     1321 cagggatgag gattataaat gcacctcatg ccaggaagca agggaatggg ctcacggatt
     1381 caggccaaac cttacattcg actatgaggt atgggatgcc ttgctgccgg aaatggcagc
     1441 ggcgggaata aacatggttg tcattgatct tggtgacgga gtggaatatg aaagtcatcc
     1501 ggaaattgcg gttaaaaacg catggaaacc ggaaaaatta aggaatgaac tgtcccggat
     1561 gcgtaagctc ggtatagaac caataccgaa actgaatttt gcaactaccc atgatatatg
     1621 gatgaaagaa tacagccgca tggtttcaac ggatatttac tatgcggtat gcagggacct
     1681 gatcagggag gtgacggagc ttttcgacag accccgtttc tttcacctgg gaatggatga
     1741 agagcttgcc tcctatcaga ccaggtttga ctatgcagtg gtccgccaga acgatctgtg
     1801 gtggggtgac ctgtattttc ttatcggaga agtggaaaaa aacggagtcc gcccatgggt
     1861 ctggtccgac tatgcatggc ataagcctga tgtgtttttc aggaaaatgc cccggtctgt
     1921 gcttcagagc aactggtact actatcagga ttttgacctg aacaaaatga aggaaccgct
     1981 caaaacatat gtaaagcttt acaatgatct ggaaaaacac ggctatgacc aggtacccac
     2041 cggaagcaac cattccgtca acgtcaattt tgaaagaact gtggaatatt gcaaaaaggt
     2101 gacagaccct tcaagactgt acggattcat gacagcaccc tggagaccaa cacttattcc
     2161 ctgtctcggc agacacaggg aggcaatcgc tgagctttca cgggccatga aaaaattcta
     2221 tcccgccttc agatgacaca atagttataa ttgataaaaa cctcttaacc catgaacaac
     2281 aatcttttca aagcattgac gcttgttacg gcaggaacgg cgattgccgg ctgcagttcc
     2341 cagcagaaaa gcaaacagcc caacattgtc tatatcatga gcgacgatca tgcctaccag
     2401 gcaatcagcg catacggata cggactcaat gagacgccca acatcgaccg tcttgcaaaa
     2461 gagggtgcaa tcttcaccag ggcatgtgtt acaaattcat taagtgcacc atgccgtgca
     2521 gtactgctga ccggcaaaca cagtttcata aacggcaagg tggataatgt aacctcattc
     2581 aactgggatc aggataattt tcccaaaatc cttcaggcaa acggctatca gacagccatg
     2641 atcggaaaga ttcatcttga cggcattccc caggggtttg acttttctat ggttctgccc
     2701 ggacaggggg aatactataa ccctgatttc cttatcgaag gcgagagagt gcggaaagaa
     2761 ggctatgtta ccgatatcat caccgaaaca gccctcgact ggctgaaaaa caaacgtgat
     2821 cccgacaagc ctttctgcct tctttatcac cacaaggcac ctcacaggaa ctggctgccg
     2881 tccccgaaat acttcaacct ttacgacgac ataacctttg aaccgccgga aaactttttc
     2941 gatgactaca gcggacgggg aagagctgca agggaacagg aaatggaaat cgacggccac
     3001 gccatgtggg ggcacgactt taagcttatt gtcggtcccg acggcgattc tacaggcttt
     3061 tacagggatc tgaaaaggat gaatcccgaa cagagagcat tgtggatgga ggcctatgaa
     3121 aaggaaaacg aggaattcct taaggcgaaa ctgatcggaa aagagcttgc atactggaag
     3181 ttcaaccgtt acatcaaaga ttatctcaga accatcaaat cgattgatga cggcgtgggt
     3241 gaattgctgg attatcttga agaggcaggc ctggatgaaa acaccattgt aatttacgca
     3301 tcggaccagg gattttatct gggtgaacac ggatggttcg acaagcgctt catgtatgaa
     3361 gaatcattca gaacacccct gctgatgagg tatccaaagg aaataaaacc cggtaccgta
     3421 atagacaggc tggtccagaa cctcgacctt gcccccacct tcctggatta tgccggagca
     3481 aaaataccgg aagacatgca gggagtgtcc ttccgcaatc ttgtcggagg caaaacggac
     3541 gagtggcgtg atgctgttta ctatacatac tatgaatatc cggaggtcca tatggtcaaa
     3601 cgccactacg gagttgccac ggaacgctat aagctgatgc acttctactt tgacattgac
     3661 gaatgggaga tgtatgacct gcagacggat ccggcggaaa tgaaaaatat atatgacgat
     3721 ccggcatatg cagaggtcag gaccatgctg cacaacaaac tggaggaact ccggaaattt
     3781 tacggcgaca gtgatgagct gaacgagaag tatcttaacc aatttcttga aaagaaaaac
     3841 ataaagagat aggattattt cccgctacac aggatttgaa acaggaattg tttctgccgg
     3901 ggaaagcctg cataccggaa catattccga aacgcggctg ccgggaaaaa gcaaaaatat
     3961 cccgttgcca tgaagcatta gcaaatcaat gacagatgat taaaaacacc acgatttttg
     4021 tcctgttcct ctcattatac gtctcaatcc tcaagggtca gacaggggat gaacttaata
     4081 ttttaaagaa taactggctt ctgtacagtg atgccgcgaa ttcattatat catcatttga
     4141 ccggcgaagc atataaatta ctggataaaa gggctgaaac cataaaccag ctccgaagtg
     4201 ccggagactg gcagaaaagg caggaagaag tccgcaggga actgtgggat attatcggtc
     4261 ccttccctga aaaaacaccc ctgaatgcaa ggataacagg aaaggtcagg aaaaaaggct
     4321 acagtattga aaacgtgatc tatgaatcat tgcccgggct gcatgttacg gcatcacttt
     4381 tcattcccga caatatcagg aagcctgccc ctgcgattct gttctgctgc ggtcacagcc
     4441 atggagccta caggctggaa tattaccagc ttcccctgct gaatcttgta aagaaaggat
     4501 ttattgtcct ggcctttgat ccgctgtcac agggtgaacg gctgcagtat ttcgaccccg
     4561 gaaagggaga atcccttatc ggcagttcaa caaaggaaca ctcctaccct gccgttcagg
     4621 tttttcttaa ttcaaaatcc atagcccgct attttatctg ggatggaata agaggaattg
     4681 attacctggt aagccgcagg gaagttgacc cgcagaggat cggagtacac gggctgtcgg
     4741 gcgggggaac gcagacagct tatatttccg cactggatga ccgggttgcg gcttcagcac
     4801 cggccggcta cataaccggt tttcgcagac ttcttcaatc cataggggtc caggacgggg
     4861 aacagaacct ttatcatcag ctgaccaggg gcatagacca tgccgattat atagagctta
     4921 gagcaccccg gcccaccctc atcatggcca caacaaggga tttcttcagc atccagggaa
     4981 cccgcgagac atatgaagag gtaaaacgtg cctataccat gcttggagct cccgggaaca
     5041 ttgaaatcac cgaggatgat tgtggtcacg attatacaag aaaaaaccgt gaggcaatgt
     5101 atgcattctt ccagaaacat ctgggattgc cgggatcttc ctctgaagaa gaagttgatt
     5161 ttgccacgga gcaggagctt cagaaaacat ccacaggaca attgtcaact tcccttgagg
     5221 gactgactgt cttcgatctt aacaaaagag aatcagaacc gctgctggcc ggacttgagg
     5281 caaaaagaaa aaactatcct gagggccttt cggatattgt tacttctgcc ggaaagctgt
     5341 ctggtttcat ttatccttcc gggggccggg aaccggtatt tgccgggcgg tttcaaagag
     5401 atgattatgc tgttgaaaaa tattttgtaa agggagaagg caattacgtc attccctatc
     5461 ttctgattaa gccccgaaag ccaggaaaca aggccgtaat ctgtctgtat cccggcggaa
     5521 aaccggaaat gattcaggaa ggaggagaga ttgaatggtt tgttagaaat ggctttacag
     5581 tgctggttcc ggatattatc ggaacaggtg agacaggacc gggtatattc cggggagatg
     5641 cagtgattat gggtgtttca aacaacatgt ggttcacatc tgtcataact ggcaaaagca
     5701 tagcaggaat ccgggcagct gatattatca gactaaggat gctgcttacc caaaaggaag
     5761 ggatcaggga agtttatggc ctggccgtaa aggaaatgac tcccgtgatg ctgcatgctt
     5821 cggcattcga tcaggaaata gcaggaacag cccttgttga acctctttcc tcgtaccgtt
     5881 ccatcgcact aaatcccttt tatcatacag gctttgtcca taatattgta gcgggagccc
     5941 ttacaggcta tgatctgcct gatcttgccg gcacaattgc accgaggaaa ctgttaatga
     6001 tcggaacaac tgacggaacg ggtgaaggaa ccggtttgga gaatactgag acggaccttg
     6061 aagtgataag aaactgctac cggcttaaaa atgcatccgg acacttgagc ataatccctg
     6121 attacaccgg agacggcctg tttgagcagt tagcagactg gattaagtaa gcgtgataag
     6181 caccggcagt tacaggaaaa gtgcaggaga atttcgcaaa ggccggtgat ctgacagttt
     6241 tattgtccgt cccggcccgg ccctatccgc atcccgggaa attttctgcc tgatgctcac
     6301 tttggtcctt tctgaaaaaa ggatattaca atgaagataa tcagataatt tatatctttg
     6361 attacaggaa caatctgata aaaaatgaaa aaatacctga tctggttgct tacaacactg
     6421 agaatccttg taggatggca tttcctttat gaaggaatta ttaagctgat gacaccgggc
     6481 tggacctcta agctctatct gatgggatcc acctggctct tctccggcat ttttcagaaa
     6541 atggctgaat cccagatgat tgtcagcatt gtggatttct tgaatatctg gggtctgata
     6601 cttatcggga tctcactgtt tatcggcctt gctgtccgtt ggtcatccat tgccggtgcc
     6661 atcctcctac tgttctattt tgttgcctac ccgcctattc cgggtcacac tttcggcacc
     6721 gcaacagaag gaagctattt gtgggtaaac aagaatctga tagaactggc cctgcttata
     6781 gtcttcgctg ctgtttcatc cgcttccttc ttcggaattg acagactgat aaaaaggtgg
     6841 aaagaggaaa aaccccatgc tccggttccg ccggttaaaa aggaaagcct tacgcttcag
     6901 cggaggcaga tgcttcgtga tctgatctca atacccttcc tcggagcttt cgcatatgca
     6961 gtgtacagga aaagaaaatg ggacagctat gaacggaaat tcctggcaga aaacactgat
     7021 gccttatccg gagcaacact caaaacattt acgttttctt cccttgatga tctgaaggga
     7081 caggtaccca ggggaagcat tgcaggaatt gaactcagca ggctcataat ggggggaaac
     7141 ctcatcggcg gctggtcaca tgcccgcgat ctgatatata ccgataagct ggtaaaggca
     7201 tatcataccg atgaaaaggt tatgatgaca atgcagctgg cggagaaatg cggaatcaat
     7261 gcaatcctca ccaatccggc tctttgcagg ataataaaca aatactggaa ggagaccggg
     7321 ggaaaaatga aattcatttc tgatggcggc ggatcggagg aatcgataag aaactcggta
     7381 aatggcggtg cctgtacggt atatgtgcac ggaggggatg cagaccgact ggtttacaac
     7441 ggcaaggttg accagatagc aaaaaatctt gaaattatcc gcagctatgg aatgccggca
     7501 ggaatcggag ctcacaggct cgaaaccatt attgcatgcg ttgaggccgg aatcataccc
     7561 gattactggg ttaaaaccat tcatcaccat aattactggt ctgcaaaggt tgaccttgaa
     7621 cgccagacaa cagtggatga aggttacaag gacaatatgt gggatttcac tccccgtgaa
     7681 acgattgaat tcatgagcag gcttgaacag ccgtggatcg gatacaagat cctggctgca
     7741 ggagcaatcc ggcccgaaga cggtttccgc tatgccttca gcaatggtgc agattttgtc
     7801 tgtgtgggaa tgtatgattt ccaggttgtg gaggactgca acattgcact tgacacactt
     7861 gcaaatgtta accgcacaag accatggaga ggataatttc attactggca atactgtctg
     7921 tactgggcgg ttttccggca caggcacaaa atattaaaac cggatccgaa aaaagtgctt
     7981 ccgggttcca gtggccggaa gggaaaaaaa tggctctgag cctgagcttc gatgacgccc
     8041 gcctgacaca gattgacaag ggtatccccc ttctggatca gtataatgtg aaagccacat
     8101 tttatgtttc accgaaagcc atgaaacaaa gggctgaagg ctggagaaaa gcagctgcaa
     8161 acggacacga cataggtaat cattcaatgg tgcatccatg ctccggcaat ttccagtggt
     8221 caagaaacag ggccctggaa gactacaccc tgaagaaaat gcgccgtgaa ctcgattcag
     8281 ccagccttgt gatcatggaa gtaacaggag tcaaacccca ttctttcgga tatccgtgcg
     8341 gacaaaaatt tgtaggcaga ggcaaaaaga ccaaaagtta tgtacccctt attgccgcga
     8401 tgtttgaaac cggcaggggc tggctcgatg aagctccgaa tgaccctgca tactgcgata
     8461 tggcaaagtt gtacggaatg gaactggatg gcaaatcctt tgaagaagtc ctgaaaatta
     8521 ttgaaacagc aaaaaaccag ggaggatggc tgatatttgc cggtcatgaa atgaatgacg
     8581 agcaggccca tcagacatcc cttctttcca cgctggaagc cctgtgccgc tatgccaccg
     8641 acccttcaaa cgaaatctgg attgacaatg tccacagtat agccagctat attaacaaaa
     8701 accgcgcggg aaacataaaa tgattttatg tgcaactaac cgtttaaaat cgctcaagta
     8761 tatcggtcac tacaatttgt tacggcattt cagtttatga caattgtttc atttagtgcc
     8821 agacctttct gtt
//
LOCUS       DAOSPX010000110         8794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7595_length_8794_cov_5.160545, whole genome shotgun sequence.
ACCESSION   DAOSPX010000110 DAOSPX010000000
VERSION     DAOSPX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7595_length_8794_cov_5.160545"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLZ75_06900"
     CDS             <1..466
                     /locus_tag="PLZ75_06900"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOS72160.1"
                     /translation="DGEGVPTVRNTIVDKGILRSFIYNTRAARRAGTESTGNAVRSGF
                     SSLPGIGTHKIYLDPGDHSRSEIIASTVKGLLVLELTGYGIDPVSGNFSGGASGLWIE
                     KGEIVHPVRGVTIAGHGFDILNALDMMGNDLDMQRSFAAPTFRIAEMQIGGK"
     gene            567..1769
                     /locus_tag="PLZ75_06905"
     CDS             567..1769
                     /locus_tag="PLZ75_06905"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOS72161.1"
                     /translation="MAAKKTKGIIAILTGGGDVPGLNPAIRAVTIRANSEGYKVIGLR
                     RGWAGITEMVRDSKADNSNCYQVLTDDIVNKAGRTGGTFLHTSRTRPSHMPKANVPEH
                     LKGTYNKDINDLTPEIIKNLEWLNVDYLIPIGGDDTLSYGVHLHRQGVKVVAIPKTMD
                     NDVPGTDYCIGFSTCVTRTIQITNNLRTSAGSHERLLVLEVFGRYAGYTAMLPTMAGA
                     ANRCVIPEYKFNIEHLARLMVEDRNNNPSKYAVVLVSEGAMFEGEEMIFADSEKDAYG
                     HAKLGGIGELVSSRLKALSSRYNNGKTINIINQKLGYLVRGGDPDFLDSIVPMAYGNL
                     AFDLILKGVHGKLVVLKNGKYGNVPLDVVTATKKFVNIEQFYNTERLRPFYHTFEAKP
                     FLIMDSNS"
     gene            1846..2778
                     /gene="rsgA"
                     /locus_tag="PLZ75_06910"
     CDS             1846..2778
                     /gene="rsgA"
                     /locus_tag="PLZ75_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOS72162.1"
                     /translation="MKQEGLVIKSTGSRYRVLYGDLRVAECAIKGKFRVRGGLKTTNP
                     VAVGDRVLFEFEPGTAAGIITDLLDRKNYILRRASNLSKQVQILAANVDHVFLMVTIS
                     HPLTQPEFIDRFLISAEAYRIPAGLIINKTDLYTAGDTEKMEHMISVYEKIGYKCYRL
                     SVREQIGLEPLIDCLKGKISLIAGNSGVGKTTLLNTLNPELNLKTAKISDYHKQGKHI
                     TTFPEMHSLPFGGYLIDSPGIKGFGMVDMQRNEIYHFFPEIFRISENCKFYNCLHLDE
                     PGCAVRLAVEEGDIDPLRYRSYLNIIEDENRKYR"
     gene            2844..5678
                     /locus_tag="PLZ75_06915"
     CDS             2844..5678
                     /locus_tag="PLZ75_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS72163.1"
                     /translation="MKKILMFSYLAVIVILLANIIYYKNLYNKQIAYIITLLDHQVQL
                     TGVMVDETNNSFSSDLNEIAFQSCLEDFFGNGDHRDAAVGKIRLFYMKYRDFISGIKI
                     YDDRRNEFTLKTDDGEWLEQQFVLHNQNEIYPRDTLLRHRQGYDYSLPIIRDNTVRGN
                     LVVTVDHSKYFSELFSAFNLRDYQWQWVINSSEEIIYSNSDSDIILNAAGIDRIRNAL
                     EGGSVGNIVHSGEINGRTRRIISSYYSTQLLQRDLALVFSAPTGYFQKYIIRNSFIIV
                     TVTLLLFLSVLYFLVRRIRKLETGKKNYEESEATLLKLIENIPAGIVVYNGKREILIA
                     NAMAARQYSYGSAAEMTGRIHPETSLTAAGEYFARNPAAGIDRAYYDIFPHDSGARIF
                     LKHTVPLDFYGEKAFLDIFTDVSELEHSRTGEAKANKAKSEFLARLSYEIRTPLTGII
                     GMTDLLNTEKLSDELRETTDVLRSSADDLMKVREDILDFSRIETGNLVLEEIPFDLRS
                     VIVHSADPVMEILIRRNISFTTDVDPAAPVSIISDPYRFRQILTNLLMHSAGNTFEGR
                     IHLSCRMLENEKGNVKLEFDLKDTGKAFDQETLNKIFGEVVNIESKILTSSDETGFAP
                     VMAAQLIRLMGGEITVTSPSGLSGGSGTAIHFTLRVYSDDRPVKETDVSNITSFSGIR
                     TLVITDPAAGDEDILDLLHKLRLNISITNFQKSTAGQIKANLESGDDKYNLIIILNDR
                     EFDAFEVVSALWDHGLHEKLILMMISNHERKGDFLKSILLAVDHYFVMPADLSGLAEA
                     LRRSFPLVEKGAGSDGDVKLRENISALVVEDNKLNQRVIARMLEKLGCSLELAADGHE
                     GISKAEEKKYDIIFMDLLLPGIDGLESAGRIMEKNPESLIVALTADNMPETRARALLS
                     GIREFISKPVRMEDLKKLLVRYFGNGIPAG"
     gene            complement(5644..6921)
                     /locus_tag="PLZ75_06920"
     CDS             complement(5644..6921)
                     /locus_tag="PLZ75_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOS72164.1"
                     /translation="MKRSDIEITAPAGSWESLMAAVQAGADSVYFGAGGLNMRSKSSF
                     NFGSGDLARIASICRENGMKSYITLNSVIYDSEREEMERMVDAAAESGVDGVIASDMS
                     VIEYAFRQGFPVHLSTQLNISNTSALKFYAKYGDVAVLARELDLEKVKMIHEAIRREN
                     ICGPGGKQVKIEMFVHGALCMAVSGKCYMSLHENNTSANRGSCYQTCRRSYIVTDRET
                     GYELEIDNEYIMSPKDLCTIGFLDRMLDAGVRVLKIEGRARSPEYVKTVTECYDEAVK
                     AVIGGNYSPEKINDWKKRLSTVFNRGFWDGYYLGQKAGEWHSRYGSAATRRKVYLGRI
                     TNYFTRPRVAEIKLENGDLRSGDLLLITGPTTGVVEYTAGEIRVDYTRTDLAPKGSYC
                     SVKINEYLRTSDKVYKWVDEPEIIPQGSRSRNT"
     gene            complement(7009..8157)
                     /locus_tag="PLZ75_06925"
     CDS             complement(7009..8157)
                     /locus_tag="PLZ75_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS72165.1"
                     /translation="MNTYRNKTPWMLFLLLFAILTGSGSLIYTRFLVETLKNEERKKA
                     ELWAEATILITIADTGQDIEFLSSIIENNITIPVILTDEADSIISSGNFDEKKLRDRD
                     YLGSQLNKIRRKNSPIEIDLGDGHINRIYYKDSIILTQLTYYPYIQTGIIILFILVAF
                     LAFRASRKAEYNQVWVSMSKETAHQLGTPVSSLAGWVEILQQDHPDLPISREIALDVE
                     RLEKITGRFSGIGSRPSLKDENLTAIILRTIEYLKSRTSSKVSFVFDSDPSTSVILPL
                     NSPLFEWVIENISRNSIDAMEGSGIITYRISENDASVVIDISDTGKGIPKRVFKKIFS
                     PGYTTKQRGWGLGLSLTKRIIEEFHDGRIYVRHSEPGIGSSIRIVLNR"
     gene            complement(8163..>8794)
                     /locus_tag="PLZ75_06930"
     CDS             complement(8163..>8794)
                     /locus_tag="PLZ75_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOS72166.1"
                     /translation="ILEQGALLNFEPVRKLSDFIDITELENIRFEELENDFFIRNNSL
                     YIPAMDVRSSAADLSVNGRHGFDNKYEYHVKILLSEILSRKVRRPKPNTNEFGAIRDD
                     GLGRTSLLLKIEDRAGEVKVSYDVRAAGTQIKNDIRKERQSLKTILNEEYGRYRDDTA
                     APAKSPGSGTPRFRVTWEEADIGDTARIRSEESRQGKQELRLRDLLKKR"
BASE COUNT         2390 a         2015 c         2067 g         2322 t
ORIGIN      
        1 cgacggggaa ggtgttccga ctgtcaggaa cacgattgtt gacaaaggta ttctcagaag
       61 ctttatatac aatacccggg cggctagaag agcaggtacg gaaagtaccg gaaatgccgt
      121 gcgcagcggc ttcagctcat tgcccgggat aggaacacac aaaatatacc tggatcccgg
      181 tgatcattcc agaagtgaaa taattgcttc aaccgtaaaa ggtttgctgg tacttgaact
      241 taccggttac ggtatcgatc cggtaagcgg aaacttcagc ggaggggcaa gcggactgtg
      301 gattgaaaag ggagagatag tccatccggt aaggggtgtg acaattgccg gacatggatt
      361 tgatattctg aatgcactgg atatgatggg aaacgacctg gatatgcaaa ggagctttgc
      421 cgcacctact ttcaggatag ctgaaatgca gatagggggc aaataacagg attaaaagga
      481 attatcttgt gcttggaatc agatcaaaaa gttataaatt tggtctctca aaaaaaataa
      541 tgctaacaaa taaaaattaa aggaaaatgg cagcgaaaaa aacaaaggga atcattgcta
      601 ttctgacagg aggcggggat gtccccgggc tgaatccggc aatcagggca gttacaatac
      661 gtgccaacag tgaaggctac aaggtaatag gacttcgcag ggggtgggca ggaattactg
      721 aaatggtaag ggacagcaag gctgacaaca gcaattgcta tcaggttctt accgatgaca
      781 ttgtgaacaa ggccggaagg accggcggga cttttctgca tacctcccgg accaggccca
      841 gccatatgcc aaaggcgaat gttcctgaac acctgaaagg tacatataac aaggacatca
      901 atgacctgac tccggaaatc atcaaaaacc tggaatggct taatgttgat tatcttatac
      961 ccatcggggg tgatgacacc ctcagctacg gggtacatct tcacaggcag ggagtcaagg
     1021 ttgttgccat accgaaaacg atggacaatg atgttcccgg aacggattac tgtatcggtt
     1081 tcagtacctg tgtaaccaga accattcaga ttacaaacaa tctgcggact tcagccggtt
     1141 cccatgaaag gcttctcgtg ctggaagtct tcgggcgcta tgccggctac actgctatgc
     1201 tgcccacaat ggccggagca gcaaaccgat gcgtaattcc cgagtataag ttcaacattg
     1261 aacacctggc caggctgatg gttgaggaca ggaacaataa tcccagcaaa tatgctgttg
     1321 tgcttgtctc cgaaggggcc atgttcgaag gtgaagaaat gatattcgcc gacagtgaaa
     1381 aggatgcata cggacatgca aaacttgggg gaataggtga actggtgtcc tcaaggctca
     1441 aggctctttc ttccagatac aacaacggaa aaaccatcaa tattataaat cagaaactcg
     1501 gatacctggt caggggcggt gatcctgact tccttgattc aattgttccc atggcatacg
     1561 gcaacctggc ttttgacctg atactgaagg gagttcacgg gaaactggtg gtcctgaaaa
     1621 acggaaaata cggaaatgtt cccctggatg ttgtcacggc cactaagaaa ttcgtgaaca
     1681 ttgaacagtt ttacaacacg gaaagactca ggccctttta tcataccttt gaagccaagc
     1741 ccttcctgat catggattcc aacagctgat aagagatcga aatattcagc aggagccact
     1801 ctgccgcagc aacagggctc ctgcaatttt caatgaattt cccgcttgaa acaggaaggt
     1861 ctggtcataa aatccaccgg cagccgctac cgggtccttt acggtgacct cagggtagcc
     1921 gaatgcgcca taaagggaaa attcagggtc aggggaggcc ttaagaccac aaaccctgta
     1981 gccgtgggcg acagggtact gtttgaattt gagcccggca ctgctgcagg aataattaca
     2041 gatcttcttg acaggaaaaa ctacatactg cggagagcat ccaatctctc aaaacaggtt
     2101 cagatccttg ctgccaatgt tgaccatgtt ttcctgatgg ttaccatttc ccacccgctt
     2161 acacagcctg aatttatcga caggtttctc ataagcgccg aagcctacag gatacccgca
     2221 ggcctgatca tcaataagac agacctttac accgccggag atactgaaaa gatggaacat
     2281 atgatttcag tgtatgagaa aatcggatat aaatgttaca gactgtcagt gagggagcag
     2341 ataggcctgg agcccctgat cgattgcctc aaggggaaaa taagcctgat tgccggaaat
     2401 tcgggcgtag gcaaaaccac acttctgaat acgcttaatc cggaactaaa tcttaaaact
     2461 gcaaagattt cagattacca caaacaggga aagcatataa ccactttccc ggaaatgcac
     2521 agtctgcctt tcggaggcta cctgatcgat tctcccggca ttaagggatt cggaatggtc
     2581 gacatgcaga gaaatgaaat ttaccatttc tttcccgaaa tattcagaat ctctgaaaac
     2641 tgtaaatttt ataactgcct tcacctggat gagcccggct gtgctgtccg gcttgctgtg
     2701 gaggaagggg atattgaccc cctgagatac aggagctatc tgaatataat cgaagatgaa
     2761 aaccggaaat acagataaat tttcagcgaa tttatcaaat aaggattatc tttatatcct
     2821 gtgaaagtca aattccggtc ctgatgaaaa agatcctgat gttcagttat ctggcagtaa
     2881 ttgtcatttt actggccaat atcatctatt ataaaaatct gtataacaag cagatcgctt
     2941 atatcatcac cctgcttgac catcaggttc agctgaccgg tgtaatggtg gatgaaacca
     3001 acaacagttt ctcatccgat ctgaatgaga tagcctttca gagctgtctt gaggactttt
     3061 tcggtaacgg ggatcacagg gatgccgctg tcggaaaaat ccggcttttc tatatgaagt
     3121 accgtgattt tatcagcggg ataaagattt atgatgacag gaggaatgaa ttcaccctca
     3181 agacagatga cggagaatgg cttgagcagc agtttgtgct gcacaatcag aatgagatct
     3241 atcccaggga cacacttttg aggcacagac agggttatga ttattccctt cccattatcc
     3301 gcgacaatac cgtgaggggc aacctcgtcg tgacggttga ccacagtaaa tacttcagtg
     3361 aactcttctc cgccttcaac ctcagggatt atcagtggca gtgggtaata aacagttctg
     3421 aagaaataat atattcaaat tcggatagtg acatcatcct taacgccgcg ggcattgaca
     3481 ggatcagaaa tgcactggag ggcggatcgg taggaaacat tgtccacagc ggggagatca
     3541 atggcaggac cagacggata atatcttcat attattcaac acagcttctt cagagggatc
     3601 tggcgcttgt attctcggca ccaaccggct acttccagaa atacataata aggaattcat
     3661 ttatcatagt cacggtcacg ctgctgcttt tcctttcagt tctttacttc ctggtccgcc
     3721 ggattcgcaa gcttgagacg gggaaaaaga attatgagga atctgaagcc acacttctga
     3781 aactcattga aaatattccg gcaggcattg tggtgtataa cggcaaaagg gaaatcctta
     3841 ttgccaacgc catggctgcc aggcaatatt catacggatc tgctgccgaa atgaccggac
     3901 ggatccatcc ggaaacatcc ctgacggctg ccggtgaata ttttgcaaga aatcctgctg
     3961 ccggtatcga ccgggcttac tacgatatat ttccccatga ctccggtgcc agaatatttc
     4021 tgaaacatac tgttcctctt gatttttatg gtgaaaaggc ctttctggac attttcaccg
     4081 atgtaagtga actggagcat tccaggacgg gtgaagcaaa ggcaaacaag gcaaagtcgg
     4141 aattcctggc aaggctgagt tatgagatcc gcacgcctct gaccggaatt atcggcatga
     4201 ctgatcttct gaatacggaa aagctcagtg atgagctgag ggaaacgact gatgttctac
     4261 ggagttcggc agacgacctg atgaaggtaa gggaagatat tctggatttt tccaggattg
     4321 agacgggtaa tctggttctg gaggaaattc cctttgacct gcgttcagta attgttcatt
     4381 ctgctgatcc ggtgatggag atacttatac ggagaaacat cagcttcacc acggatgttg
     4441 atcccgctgc cccggtcagc attatttctg acccctacag gttccggcag attcttacaa
     4501 accttctgat gcattccgcc ggaaacacat ttgagggccg gatccatctc agctgcagga
     4561 tgctggaaaa tgaaaagggt aatgtaaaac tggaatttga tcttaaggat acgggaaagg
     4621 cctttgatca ggaaacactg aataaaatat tcggcgaagt agtaaatatt gaatccaaaa
     4681 ttctgaccag cagtgatgaa acgggattcg ctcctgtgat ggctgcccag cttatcaggc
     4741 tcatgggcgg tgaaattacg gttactagcc cttcaggcct ttcgggtgga tcgggtacgg
     4801 caatacactt taccctcagg gtgtattccg atgacaggcc ggtgaaggaa acggatgtca
     4861 gcaacatcac ttccttcagc gggataagga ctcttgtaat cactgatcct gctgcagggg
     4921 atgaggacat ccttgacctt ctgcataaac tacgcctgaa catatctatt accaactttc
     4981 agaaatcaac agcagggcag attaaagcca atcttgaatc cggcgacgat aaatataatc
     5041 tgatcattat tttgaatgac agggaattcg atgcttttga agttgtttcg gcactctggg
     5101 accatggtct tcatgaaaag ctgatcctga tgatgataag caatcatgaa aggaagggtg
     5161 attttcttaa atccattttg ctggctgtcg atcactattt tgtaatgccg gccgatctct
     5221 ccggactggc agaggcactc agaaggagtt tccccctggt tgaaaaagga gccggatcag
     5281 acggtgatgt gaaattaagg gaaaacatct ctgctctggt tgttgaggat aataagctga
     5341 atcagagagt cattgccaga atgctggaaa agcttggatg ttcgcttgaa ttggctgcag
     5401 acgggcatga agggataagt aaagcggaag agaaaaaata cgatataatt tttatggatc
     5461 ttcttcttcc gggaattgac ggccttgaat cggccggccg gattatggaa aaaaatcctg
     5521 aaagcctgat cgttgccctg acagcagaca acatgcccga aacgcgggcc agggctttat
     5581 tgtcaggtat aagggaattc atatccaaac cggtaaggat ggaggatctg aaaaaacttc
     5641 tggtcaggta tttcgggaac gggatccctg cgggatgatt tccggttcat ccacccactt
     5701 gtagactttg tcggaagtcc ggagatattc atttattttc acagagcagt atgatccctt
     5761 cggtgccagg tcggttctgg tataatcaac ccttatttcc cctgccgtgt attcaacgac
     5821 acctgttgtt ggaccggtta tcagcagcag gtcaccgctt ctgagatcgc cgttttcaag
     5881 ttttatttcc gcaacccgcg gtctggtgaa atagttggtg attctgccca ggtacacttt
     5941 tcgcctggtg gcagctgatc cgtaacggct atgccattcc ccggcctttt gtcccaggta
     6001 gtagccatcc cagaaacccc tgttgaaaac cgtggagagc ctttttttcc agtcatttat
     6061 tttctcaggg gaatagtttc cgccgataac cgcctttacc gcctcatcgt agcattccgt
     6121 tacagttttt acatattccg gtgagcgtgc ccgaccctca atcttaagca cccttacgcc
     6181 ggcatccagc attctgtcaa ggaagcctat tgtacacaag tccttgggag acattatgta
     6241 ttcattgtct atctcaagtt catatcctgt ttccctgtcc gttacaatgt atgacctgcg
     6301 gcatgtctgg taacagcttc ccctgttggc tgatgtattg ttttcatgaa ggctcatgta
     6361 gcattttccc gaaacagcca tgcacagggc tccgtggacg aacatttcaa tctttacctg
     6421 cttccctccg ggtccgcata tgttttcccg ccttattgcc tcatgaatca tttttacctt
     6481 ttcaaggtca agttcccttg ccagtacggc aacatccccg tatttggcgt aaaacttcag
     6541 tgcggaggta ttcgagatat tgagctgggt tgacaggtga acgggaaagc cctgcctgaa
     6601 ggcatattct ataaccgaca tgtcggaggc tatcaccccg tcaacaccgc tttcagcagc
     6661 agcatcaacc atcctttcca tttcctccct ttcactgtca taaatgacag aattgagagt
     6721 gatatagctt ttcatgccgt tttccctgca tattgaagca atcctggcga gatcgccgga
     6781 tccgaagttg aatgaagact ttgacctcat gtttagtcct cccgcaccga aatataccga
     6841 gtcggcacct gcctgtacgg ctgccatgag cgattcccag gaaccagcag gcgctgttat
     6901 ttcaatatca ctccttttca tcttgtctgc tgaaaaacca taaacagcat tttactgaag
     6961 aaatgcaatc cgcagataag cttcatttga ttaaaacact gctgacggtt atctgttcaa
     7021 tacaattcta attgaggaac caatgccggg ttcagaatgc ctcacataaa tccttccgtc
     7081 gtgaaactcc tcaattatcc ttttggtaag cgagaggccg agtccccacc ccctttgctt
     7141 tgttgtgtaa cccggactga atatcttctt gaacaccctt ttgggaattc ccttacccgt
     7201 gtcgcttatg tcaataacaa cactggcatc gttttcggat atacggtatg tgatgatgcc
     7261 gctgccttcc attgcatcta ttgaatttct tgagatattc tcaataaccc attcgaaaag
     7321 aggactatta agaggaagaa taacacttgt tgaaggatcc gaatcaaaaa caaagctcac
     7381 cttggaagag gtgcgggatt tcaggtattc gatggtacgg aggattatgg cggtaaggtt
     7441 ctcatccttc agggaagggc gggatccgat tccggagaac cggccggtaa ttttttccag
     7501 tctttccaca tccagtgcaa tttcccttga tatgggcagg tccggatggt cctgctgaag
     7561 tatctcaacc cagcctgcca gggaggaaac cggtgtcccg agctgatgtg ccgtttcttt
     7621 cgacatgctc acccacacct gattgtattc cgcctttctt gaggcccgga atgcaagaaa
     7681 tgccaccagg atgaaaagta ttataatccc cgtctgaata tagggatagt aggtaagctg
     7741 tgtaagtata atgctgtcct tatagtatat acggtttata tgaccgtcgc ccagatcgat
     7801 ttcaatggga ctgttcttcc tgcgtatttt attgagctgg gaaccgagat aatccctgtc
     7861 gcgcagtttc ttttcgtcaa aattgccgga acttattatg ctgtctgcct catctgtgag
     7921 aattaccgga atggttatat tgttctcaat tatcgaggaa aggaattcaa tatcctgacc
     7981 ggtatcggca atagttatca gaattgttgc ctcagcccac agttccgcct tttttctctc
     8041 ttcattcttc agtgtctcaa caagaaaacg ggtatatatc agggatcctg aacccgtcag
     8101 tattgcgaac aggagaagga aaagcatcca tggtgtctta ttacggtaag tgttcaacgg
     8161 aattatcttt ttttcagtaa atccctcaat cttaattcct gttttccctg ccgggattct
     8221 tcagaccgga tcctggcagt gtcgcctatg tctgcctctt cccatgtaac cctgaatctg
     8281 ggcgttccgg acccgggaga tttagcagga gcagcggtat catccctgta ccgtccgtat
     8341 tcctcattga gtattgtctt aagcgactgt ctttccttcc tgatgtcgtt ctttatctgc
     8401 gtacccgcag ccctgacatc gtagcttacc tttacttctc cggccctgtc ctctatcttc
     8461 aggaggaggg aagtcctccc gagaccatcg tcccttatgg ctccgaattc atttgtattc
     8521 ggtttcggcc gtcttacctt cctgctgaga atctcggaaa gcagtatttt tacatggtat
     8581 tcatatttat tatcgaaacc gtgcctgccg tttacggaga ggtcagcggc cgacgacctg
     8641 acatccatgg cgggaatgta aagggaattg ttacgtataa agaaatcgtt ttcgagttct
     8701 tcaaaacgta tattttcaag ttccgtaata tcaataaaat ctgacagttt cctgaccggt
     8761 tcaaaattca gaagagctcc ctgctccagg atat
//
LOCUS       DAOSPX010000111         8787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7605_length_8787_cov_4.724347, whole genome shotgun sequence.
ACCESSION   DAOSPX010000111 DAOSPX010000000
VERSION     DAOSPX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7605_length_8787_cov_4.724347"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLZ75_06935"
     CDS             complement(<1..680)
                     /locus_tag="PLZ75_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72167.1"
                     /translation="MRYITVILFIVSATLSHSQISVKYPNDAGIEKDPDVLYVEKFND
                     GLNNILGRYDEIVNSGGMTLDRDIPSGSLEPYSLRITSIPGENMGGHLYKSFRPGFDN
                     TVYLRYYVKHPSIPQGYYGHQGLWFGGYNPVLDYPYPRAGICGLGSSRISIAFENVWR
                     SGSRPGMDAYIYWGDMKSYDEGATCFGNTMITEGRRDYQQAPAPDAPLCDFDEWMCIE
                     VMIRLNDPV"
     gene            893..1525
                     /locus_tag="PLZ75_06940"
     CDS             893..1525
                     /locus_tag="PLZ75_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOS72168.1"
                     /translation="MLRNTILKIIYNDIMNSGQRKKNYIIKAISVFKQDGLRLSLEEV
                     AGKMGITKKTLYNHFSSKDELLRECILSISSDMQQAVRGLDDPDHPAIENLRYSFLNL
                     NLFFTELSPVFFYDIMRLNPNQATREHFTGSELFRKKLEANLRQGIKEGVYRKNLDVE
                     FISRYISYSVFGFYINSVINNNPHIPKSYFKDTAEYNLGAIVSEKGKQLL"
     gene            1541..2923
                     /locus_tag="PLZ75_06945"
     CDS             1541..2923
                     /locus_tag="PLZ75_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS72169.1"
                     /translation="MKIQFIKSVFILFGLLPGATVLFSQETYSLRQCLEYALDNNRNL
                     KKSNYDREKAAYARQEVLGSLLPQISGSANLNDNLKKARFIMPNFVNSMLPPSAQDPD
                     AEKYMIIEMGTTYNANVGVSLSQQVLNLGLFNVLEIAKTAEKMAALGVEADEEEVISQ
                     TVNLFYAVQSTEYAVTQMDRSVELVNRMLETMEENHRNGLVRKVDVDRLKVNLVNLTT
                     QRVAVKNAAEVQKNLLKLQMGFDVGNPIDIQAIDLALFEEKVKDNYESPFSLKNQTSF
                     LLLMEKMDMVKLQKKSAVYEYMPTLSLMFNYQYNGVSDEFFRGETNYWYPGSVVGLSL
                     RIPVFSGFSRRYKISQNSMELQKAREDAELLGQSLNMAFMNARIKLADMRNTINLQRD
                     NQSLAEEVFRVTENNFMLGLSSMSDILNAAQSLVQAQLSYAGALNDYMKAWVDLKKAS
                     GSIRDLLTEN"
     gene            3028..3996
                     /locus_tag="PLZ75_06950"
     CDS             3028..3996
                     /locus_tag="PLZ75_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS72170.1"
                     /translation="MKKTQMAVGASSAVAVKAEVVRDRSYSVSFSASGVLKALRDLQF
                     VSDVSGRILEIYADEGSHVTKGKVLLQLDSELLLADVSSREAAYEGLKKDYERFKSAN
                     EQGGVTDQQLDNIRTQMITAENLLITSKRRLADASVKSPITGTIYKRHVEVGSYLNPG
                     TPLFDIIDDSQLKATCYATEKQIQGIRKGLEVTLVSETFPGESFTGKITFIGEKADRS
                     LNFPVEVSLTGSRKGLKPGMFVTVLFESGTQKNGILIPRSAISGSVQGASVFVIENSV
                     AKKRNTVIGDIVGERMEVLQGLNPGDSIVTGGLINVSDGTKVRSIP"
     gene            4021..7134
                     /locus_tag="PLZ75_06955"
     CDS             4021..7134
                     /locus_tag="PLZ75_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOS72171.1"
                     /translation="MKITELSIKRPVYVIVLFLLLTVLGYLSFRGLGAELMPKFTPPI
                     LNVQIVYPGASPSEVENSLTRKAEDALSGMEGIDQLQSFSFEGMSMLIVSFKYGTDIN
                     KAVTDAQNLLDAKRSDLPRDILSPVISKISVDEKPVMILSATSNIEATDFYDLIDKRI
                     LPEFSRLNGVAKISLVGGMQREIQINLNQEKLTAFGLTPLMIQAAIRAANLDFPTGYL
                     HGSKSQIAVRLSGKLTNIDELRNLIVSTGRAGTQIRLGDVADIADAVKDPVTLGRING
                     QDAILISLQKQSDANAIRVSDEVMKTISRLENTHSAEGLQISVAQDTTTFTHQSINSV
                     ITDLFLAILMVSLVILLFLHNVRNALIVMVVVPVSLISTFIGMKLFSFTLNLMSLLAL
                     SLVIGVLVDDAIVVIENVYRHIEMGKNRVKATFEALKEIGFTVISVTIAIVMVFLPVI
                     FTDTLVSDILRQFCAVIVISILFSLLAALTLVPLLTSRFGNIQEIKGENIFGKLLKGF
                     ENAISRFSHWISDILKWGLGHKRVVGLVVLCLTLAVMSLFPLGFINFEFQPYIDRGEF
                     IVQLEMPKDISMEEANALVQKAENWFMSRPEIKDVITMVGLTSDNTQSTKGTPYLAEL
                     DVKLVKQKEGTETYITRIRKPLSDYLIDAKVNIFSVSLTGTASKAAVEYIISGNNSDT
                     VMFYAEKALDALGSIPGVMQHELSVENTTPEITVTVNRDKMAELGLSLENVGMMMQMS
                     FQGNDQLKYTAGEYEYDINIRADRMYREQADNVSGLVFINSRGENVRLSQFAAVSPGT
                     GPNRLERYDRNSSVTLRSQVFGVPAGAISKQFMAKMDNMYKPKGLRIEATGDMKKMSE
                     SMNVLTTALIMSLLLIYLSLVVLYNNWTDPLVIMFSIPFSIVGAILALALTNTAMSIY
                     AMLGLIMLVGLVAKNAILLVDFANDALREGRRVDEALIQAVRIRTRPILMTALSTTIG
                     MLPIALSTGSGAELRNGMAWVIIGGMLMSTLLTLVVVPVVYKILYPIRMAGKEKTDIE
                     KLMYAVD"
     gene            complement(7196..>8787)
                     /locus_tag="PLZ75_06960"
     CDS             complement(7196..>8787)
                     /locus_tag="PLZ75_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOS72172.1"
                     /translation="IGLAQEEDGYLYTNRTIAEMHGGEGLHPWASKNRWELDSILSHE
                     LYNAGHLFEAAVAHYQATGKKNLLDIAVKSADLIDKDFGHNKIKVYPGHQGIEMGLVK
                     LYRATGDRRYLDLGRFFLDIRGPGGQEYNQAHKRVVDQTEATGHSVRAAYMYSGMADI
                     AAIQGDEAYLKAIAEIWEDIVYRKMYITGGIGATGGNEGFAGPYVLPNLTAYCETCAS
                     IANIFTNHRLFLLHGRGKYIDILEKTLYNSMLSGVSLSADRFFYPNPLESDGRHERSE
                     WFGCACCPSNIARFVPAIPGYVYASEGRDIYVNLYISNEAEIFSGKKKVNLVQQADFP
                     WDGKVKINVNPARSGNFAIRLRIPGWVMNEAIPGDLYRFDDRTGDSFKISVNGIPVDN
                     TPEDGYVSISRKWSEGDVIELEFPMPVRIVVADERVEEDRDRIAIQRGPVVYCAEWPD
                     NEDMNVLDLVIDRNGPLKTEFIPSLLDGTQVIRARAYPAETDSGGSTLIPAGRTITLI
                     PYALWNNRGPGQMRVWLPVKD"
BASE COUNT         2467 a         2044 c         1994 g         2282 t
ORIGIN      
        1 acaggatcat taagcctgat cattacctct atgcacatcc attcgtcaaa atcacaaagc
       61 ggtgcatccg gcgccggtgc ctgctgataa tctcttcttc cctccgtaat cattgtgttt
      121 ccaaaacagg tagccccctc atcataactt ttcatgtcac cccagtatat atatgcatcc
      181 atacccggcc gggaaccgct gcgccatacg ttttcaaagg ctatacttat ccggctgctg
      241 ccaagaccac atattccggc tcgcggataa ggataatcca gtaccggatt gtatccgccg
      301 aaccaaagtc cctgatgccc gtaataaccc tggggaattg aaggatgttt cacgtaatat
      361 ctcagataaa ctgtgttatc gaatcccggc ctgaatgatt tgtataaatg ccctcccatg
      421 ttttctcccg gaatgctcgt gattctaagg gaatagggtt ccaggctgcc ggaaggaata
      481 tccctgtcaa gcgtcattcc cccggagttt acaatttcat cataacgacc aaggatatta
      541 ttcagaccat cattgaattt ctccacatat aaaacatccg gatccttttc tatgccggca
      601 tcgttgggat atttaaccga aatctgggaa tgggacagcg ttgctgaaac aatgaataaa
      661 ataactgtaa tatatctcat aatagaacct gtttgtaaac cgggccataa tatctatttg
      721 taccggtctt tccggacctg agatcaaatt tacattaatt ttcaattgtc tccggtatca
      781 atcattattt cgaatccgga acagcggaat tttacagatg cctgcccgaa atacttctta
      841 tccggaaatt attatactct ttttatacgt ttattgtata aatagtgtaa ttttgttacg
      901 aaacactatc ctgaaaataa tttacaacga cattatgaat tccggtcagc gaaaaaaaaa
      961 ctacataatc aaagcgatat cagtatttaa acaggacggt ctgcgccttt ccctggagga
     1021 ggttgcgggg aagatgggca taacgaaaaa aaccctgtat aaccattttt catccaaaga
     1081 tgaactactg agagaatgca tcttatccat ttcatcagat atgcagcaag ctgtcagagg
     1141 gcttgatgac cctgaccatc cggcaattga aaatctgcga tacagttttt tgaatctgaa
     1201 tcttttcttt accgagctga gccctgtttt tttctacgat attatgcgcc tgaaccccaa
     1261 tcaggcaaca agggagcatt tcaccggatc ggaattattc agaaaaaaac tcgaggcaaa
     1321 tctcaggcag ggaattaagg agggtgttta cagaaagaat ctggatgtgg aattcatcag
     1381 ccggtacatt tcctattctg ttttcggatt ttacataaac agtgtgatca acaataatcc
     1441 tcatatcccg aaatcctatt ttaaagatac ggcagagtac aatctgggag ccatcgtttc
     1501 ggaaaaagga aaacagttat tgtaaaataa tcttctcatt atgaaaatac agtttattaa
     1561 atcagtgttt atattgttcg gtctcctgcc tggtgcaacg gtcttgtttt cgcaggaaac
     1621 ttattcactc cgtcaatgcc tggaatatgc cctcgataac aaccggaatc tgaaaaagtc
     1681 aaattacgac agggaaaagg cagcttacgc ccgccaggag gtattgggct cgcttctgcc
     1741 ccagatcagt ggatcggcaa acctgaacga caatctcaaa aaggccaggt tcatcatgcc
     1801 caatttcgtg aacagcatgc tgccaccttc agcccaggat cccgatgcgg agaaatacat
     1861 gataattgag atgggtacaa cttacaatgc caatgtagga gtgtcactca gtcagcaggt
     1921 actgaacctg ggccttttca acgtgctgga aatcgcgaag actgcagaaa aaatggcggc
     1981 cctgggagtt gaagccgacg aagaagaagt gatcagccag accgtaaatc ttttttacgc
     2041 cgtgcagtcc acagaatatg cagtcacaca aatggacagg agcgttgaac tggtgaacag
     2101 gatgctggaa acaatggaag agaaccacag gaacggactg gtcaggaaag ttgatgttga
     2161 caggctgaaa gtaaatcttg tgaacctgac tacgcaaagg gttgccgtta aaaacgctgc
     2221 agaagtacag aaaaacctgc tgaaactgca aatggggttc gatgtaggca atcctattga
     2281 tattcaggct attgatctgg ccctttttga agaaaaggta aaagacaatt acgaatcacc
     2341 attttctttg aaaaaccaga catcatttct ccttcttatg gagaaaatgg atatggtgaa
     2401 actgcagaaa aaatcagccg tttatgaata catgcccacc ctttcactta tgttcaacta
     2461 ccagtataac ggcgtgagcg atgaattttt caggggggaa accaactact ggtatccggg
     2521 ctcagtggtc ggattaagcc tgagaatacc agtcttcagc ggattctcac gcagatacaa
     2581 aatatcgcaa aacagtatgg aactgcaaaa agccagggaa gatgccgaac tgctcggaca
     2641 atcattaaac atggcattta tgaatgccag gatcaaactt gccgatatgc ggaatactat
     2701 taacctgcaa cgtgataatc agagtcttgc cgaggaagta ttcagggtga ctgaaaataa
     2761 tttcatgcta ggactgtcat ctatgtctga cattctcaat gcggcccaat cgcttgtgca
     2821 agcacaactg agctatgccg gtgccctgaa tgattatatg aaagcctggg ttgacctgaa
     2881 gaaggcaagt ggcagtatcc gcgatttatt gacagaaaat taaaaacata accagggact
     2941 gaaatgaaaa gagtaattca aatcctatcg ctgtgtgcag tattagctgc aatcgtagcc
     3001 atcctcattt ccaacaaaag aacgacaatg aaaaaaacgc aaatggcagt gggtgcttct
     3061 tctgccgttg ccgtaaaagc tgaagttgtc agagaccgtt catactctgt gagtttttca
     3121 gccagcggtg tgctgaaagc actgcgcgac cttcaattcg tgtcggatgt atcggggcgt
     3181 attctggaaa tttatgccga tgaaggaagt catgtaacaa aagggaaagt gctgctgcaa
     3241 ctcgacagcg aattgcttct agccgatgtt tcatcacggg aggccgcata tgaaggactt
     3301 aaaaaggact atgaacggtt taaaagtgca aatgaacagg gaggtgtcac agaccagcag
     3361 ctggacaata tccgcaccca gatgattaca gcggaaaacc ttcttataac aagcaaacgg
     3421 cgtcttgccg atgcatcagt gaaatcaccc attacaggaa caatctataa acgtcatgtg
     3481 gaagtgggca gttatctgaa tcccggcact ccgctattcg atattataga tgactcacaa
     3541 ctgaaagcga cctgttacgc taccgaaaaa cagattcagg gaatcagaaa aggactggaa
     3601 gtaaccctgg taagcgaaac attcccgggg gaaagcttca ccggaaaaat cacatttatc
     3661 ggtgaaaaag ccgaccgttc gctcaatttt ccggtagaag tcagcctgac cggttccagg
     3721 aaaggactga aaccgggtat gtttgtcaca gtgctctttg aatcggggac acaaaaaaac
     3781 ggtatcctca tcccgcgcag tgccatcagc ggaagtgttc agggagcaag cgtgttcgtc
     3841 attgagaaca gtgtcgcaaa aaaaagaaat acggtaatcg gcgatatagt cggggaaaga
     3901 atggaggtcc ttcagggatt gaatcccgga gacagtatag tgacaggcgg attgataaat
     3961 gtgtcagacg gtaccaaagt gagaagcatc ccctgatgat gaattttaaa tattcagaag
     4021 atgaaaataa cagaattatc cataaaacgc ccggtttacg tcattgtgct cttcctgctg
     4081 ctgacagttc tcggatacct gagtttcagg ggactgggtg cggaactgat gccaaaattt
     4141 actcctccca tacttaatgt ccagattgtg tatccgggag catcgccctc tgaagtggaa
     4201 aactcactca cacgcaaggc agaggatgct ctttcaggta tggaaggaat cgaccagctt
     4261 cagtcttttt catttgaagg aatgtcaatg ctcattgtat catttaaata cgggaccgat
     4321 atcaacaaag ctgttaccga tgcacagaac cttcttgatg caaagcgctc agatcttccc
     4381 cgggatattc tttcgcccgt tatatcaaag atatcggttg acgaaaaacc tgtcatgata
     4441 ctttcggcaa cctccaatat tgaagcgaca gatttctacg atctgataga caaacgtata
     4501 ctgcctgagt tttcacgcct gaacggagtg gcaaaaatat cacttgtggg aggtatgcaa
     4561 cgcgagatac aaattaattt aaaccaggaa aagctgacgg ccttcggcct tacacccctg
     4621 atgattcagg ctgcaatccg agcggccaat cttgactttc cgaccggata ccttcatggc
     4681 agcaaatcac agatagccgt cagactgtcg ggaaaactga ccaatattga cgaactgagg
     4741 aatctgatcg tcagcaccgg ccgggcaggt acacagatcc gtctgggaga tgtggcggat
     4801 attgcggatg ccgtgaagga tcctgtgaca cttggacgta ttaacggaca ggatgccatt
     4861 ctgatcagcc tgcagaagca atccgatgcc aatgccatca gggtaagcga cgaagtgatg
     4921 aaaacaataa gccggctgga gaatactcat tccgccgaag gtttgcagat cagtgtggca
     4981 caggacacta ctacttttac tcatcagtca attaattccg tcatcaccga tttgtttctt
     5041 gctattctga tggtttcgct ggtgatactg ctgtttctgc ataatgtgcg caacgctctt
     5101 atcgtaatgg tggtcgttcc ggtttcactc atctccacat ttatcggaat gaagcttttc
     5161 agtttcacac tcaacctgat gtctctgctt gccctgtcac tggtcatagg tgtactggtt
     5221 gacgatgcca ttgttgtcat tgagaatgtg taccggcata tcgaaatggg aaaaaaccgt
     5281 gtgaaagcca ctttcgaggc attgaaggaa atcgggttta ccgttatttc cgttaccatt
     5341 gcaatagtga tggtgtttct gccggtaata tttaccgaca cccttgtttc cgatatattg
     5401 cggcagtttt gtgcggttat tgttatttcc atacttttca gcctgctggc tgctcttacc
     5461 ctggtgccac ttcttacttc ccggttcggg aacattcagg aaatcaaggg ggaaaacatt
     5521 tttggaaagc tgctgaaagg ctttgaaaat gcaatttccc gtttcagcca ctggatctcg
     5581 gacatcctga aatggggtct gggacacaaa cgcgtggttg gtctggtggt gctttgcctg
     5641 acacttgccg taatgtcgct cttccccctg ggatttatca attttgagtt tcagccttac
     5701 atagaccgcg gagagtttat tgttcagctg gaaatgccga aggatatttc aatggaggaa
     5761 gcaaatgccc tggtacagaa agcagaaaac tggtttatga gccgtcccga aatcaaggat
     5821 gtcattacaa tggtaggtct taccagcgat aacacccaga gtaccaaagg cactccctac
     5881 ctggctgaac tggacgtaaa actggtaaag cagaaagagg gaacggaaac ctatatcacc
     5941 cggatccgta agcctctttc cgattacctg attgatgcca aagtgaatat tttcagcgtg
     6001 agcctgaccg gaacagcatc aaaagcagcc gtggaataca tcatttccgg taacaacagc
     6061 gatacggtga tgttttatgc cgaaaaagca ctggatgccc tgggctcaat tcccggtgtg
     6121 atgcagcatg aactctcggt tgaaaataca acacccgaaa taaccgtaac agtaaacagg
     6181 gataaaatgg cggaactcgg actttcgctc gagaacgtgg gaatgatgat gcagatgagc
     6241 tttcagggaa atgatcagct gaaatatacc gccggggagt atgagtatga tatcaatatc
     6301 cgtgctgaca gaatgtatcg agaacaggca gataatgttt ccggtctggt atttatcaac
     6361 agccgcggtg aaaatgtaag gttgtcacag tttgccgctg tttcgcccgg aacaggacca
     6421 aaccgcctgg agcgctatga ccgtaacagt tcagtgacac tccgttcaca agtcttcgga
     6481 gtccctgccg gcgccatatc aaagcagttc atggcaaaaa tggataatat gtacaagcca
     6541 aaaggtttac ggattgaagc aacaggagac atgaagaaga tgagtgaatc catgaacgtg
     6601 ctcacaacgg ctctcataat gtcattgttg cttatctacc tgtcactggt tgtgttgtat
     6661 aacaactgga ctgaccctct tgtaatcatg ttttccattc ccttttccat tgtgggtgcc
     6721 atactcgcac tggctctcac caatactgcc atgagtatct atgccatgct cggactgata
     6781 atgcttgtcg ggctggtggc caaaaatgcc atactgctgg ttgacttcgc caatgatgcc
     6841 ctacgcgaag gcaggagggt tgatgaagct ctgattcagg ccgtacggat acgtacacgt
     6901 cccattctga tgacagccct gtcaacaacg ataggaatgt tgcccattgc gctttccaca
     6961 ggttcaggtg ccgagctgcg caacggaatg gcatgggtga tcatcggtgg aatgctgatg
     7021 tcaacacttc tgaccctggt ggtggtgcct gttgtctata aaatccttta tcccatccgt
     7081 atggccggaa aggaaaagac tgatattgaa aaacttatgt atgccgtgga ctgattgtta
     7141 catcattgat tagcacgcca ttgattgtta tatcattgat tacctgttcg ctactctagt
     7201 ccttcaccgg cagccagacc ctcatttgtc ccggacccct gttgttccac agggcatagg
     7261 gaataagcgt gattgtccgc ccggcaggga tcagggtgct gccaccggaa tccgtttcag
     7321 ccggataagc tctggcccga atgacctgtg tgccgtcaag aagcgaggga ataaactccg
     7381 tttttaatgg cccgttcctg tcgatcacca gatcaaggac attcatatcc tcattgtcgg
     7441 gccattcggc acagtaaacc actggtccgc gctgtatggc aatcctgtcc ctgtcttcct
     7501 ccaccctttc atctgcaacg acaatcctta caggcatggg aaattccagc tctataacat
     7561 cgccttccga ccatttgcgg ctgatactga cataaccatc ctccggtgta ttatccaccg
     7621 gaattccgtt gactgatatt ttaaaactgt cgcctgtcct gtcatcaaaa cggtacaggt
     7681 caccgggaat ggcttcattc atcacccagc cgggtatgcg gaggcgtatc gcaaaattcc
     7741 cggaccgcgc gggattgaca ttgattttaa ctttcccgtc ccaggggaaa tctgcctgct
     7801 gaaccagatt tacctttttc tttcccgaaa aaatctcagc ttcatttgaa atatacaggt
     7861 ttacatatat atcccggcct tccgaagcat atacatatcc ggggatcgca ggcacaaacc
     7921 tggcaatatt tgacggacag catgcgcatc cgaaccattc gctcctttca tgtcttccat
     7981 ctgattcgag aggattcgga tagaagaatc tgtcggctga aagggacaca cccgacagca
     8041 tcgagttata cagtgttttt tcaagtatgt caatatattt acccctgcca tgtaacagaa
     8101 aaagtctgtg gtttgtaaaa atattggcga tggaagcaca ggtttcacag tatgcagtca
     8161 gattgggcaa cacataggga ccggcgaaac cctcatttcc gcccgttgcc ccgatacccc
     8221 cggtaatgta catcttgcgg tacacaatat cttcccatat ctcagcaata gctttcaggt
     8281 aagcttcatc cccttgtatg gcggcaatat ccgccatacc cgaatacata taggctgccc
     8341 tgacagaatg cccggtcgcc tccgtctgat caaccaccct tttgtgagcc tggttgtatt
     8401 cctgcccgcc cgggcccctt atatcgagaa aaaacctgcc cagatcgaga tatctcctgt
     8461 cgccggtggc ccggtataat ttcaccagac ccatctcgat tccctggtga ccgggatata
     8521 ccttaatctt gttgtgacca aaatccttat caatcagatc tgctgattta acagctatgt
     8581 caagaaggtt ttttttgcct gttgcctgat aatgtgcaac agccgcctca aaaagatggc
     8641 ctgcattata taattcatga ctcaggattg aatccagctc ccatctgttt ttggatgccc
     8701 agggatgaag cccttcgcct ccgtgcattt cagctattgt cctgttcgtg taaagatagc
     8761 cgtcttcttc ctgggccagg cctatcc
//
LOCUS       DAOSPX010000112         8749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7646_length_8749_cov_4.821256, whole genome shotgun sequence.
ACCESSION   DAOSPX010000112 DAOSPX010000000
VERSION     DAOSPX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7646_length_8749_cov_4.821256"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..204)
                     /locus_tag="PLZ75_06965"
     tRNA            complement(119..204)
                     /locus_tag="PLZ75_06965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Leu,seq:taa)
     gene            complement(339..3113)
                     /locus_tag="PLZ75_06970"
     CDS             complement(339..3113)
                     /locus_tag="PLZ75_06970"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOS72173.1"
                     /translation="MTLRSFISGKPENHQDGFSIPHEKYRLANGLTVILNKDRSNPIT
                     SLVIFYHVGSGRESPGKTGFAHLFEHMMFQRSENVGEDQFFRYIEGAGGNLNGGTSFD
                     QTVYFEVVPKNALELAMWLESDRMGFMINSVTQSSFSLQQNVVQNEKRQYVDNMPYGF
                     TDHVILKNLYPAGHPYSWDVIGEMEDIKNAGIDDVREFHRKYYVPNNATLVVSGDFET
                     EEVKFLIEKYFGEIPAGNIVTKPEPANIILNETRKLYHEDSFANAPQFNMVFPTCENF
                     TRDSYSLNVLAQLLGQGKKSPLHQVLVREKQLTSAVTAANQALELTGNLRISITAKPD
                     TSLNEVEKGIFEAFSRFRKEGFTEKDLERIKARLETEFYNTISSVLFKSFMLAFYDVM
                     AGSPDYLISELDRMKSVTAEDVWDVYNRYIEGKNYVATSFVPKGQLHLAAEGSVNAGI
                     AEEDITRAAQVDIEADMEEEIIKTPSGFDRSAAPVPGPDPEIRLPQVWNSVFPNGMRI
                     HGIERHELPMVSYSLTIRGGHMLDDLKKAGTAKFMASMLNEGTAKKTPEELEEAIQLL
                     GATFEISGDNEDITFSVNSLSRNFEKTVELIEEMLLEPRWDEEQFALGKIRIINEIRR
                     NMSDPNYLADRVFNNLVFGRENILSVHADGTAGSVESITIDDLREFYDKAVSPAVAEF
                     MIVGDVSRPRVEKALEELGKKWKKKHVAIPEPVFPAPPEKASLYFVDVPGAKQSVIKA
                     GYLALPRGHEDFYKSVTANFMLGGGASARLFMVLREEKGYTYGAYSAFKGGRQYGTFS
                     ARTSVRSDATPDSVRLFRDIMLEYRKGQSSETVEFTRDSLLKANALRFETNEALLNML
                     RTMILNGLPGDYIKQEEAYLRKLTAEQMNTVIRKYIDPLKMYYVVVGDAKTLLKELEK
                     VGFGKPVLFQ"
     gene            complement(3268..6072)
                     /locus_tag="PLZ75_06975"
     CDS             complement(3268..6072)
                     /locus_tag="PLZ75_06975"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOS72174.1"
                     /translation="MKKLFSLPLIFLVAIMFSCNSGNRKTELSIPFEKYTLPNGLTVV
                     LNVDKSDPIAAVAIYYHVGSSREEPGKTGFAHLFEHMMFQRSENVGEDQLFREIQGAG
                     GTLNGSTSQDRTNYYEVVPKNALELVLWLESDRMGYLANTVTKSSLANQQNVVQNEKR
                     QSVDNAAYGFNQEIIAKNLYPEGHPYSWTVIGLMEDLTNATIEDVKAFHGKYYVPNNA
                     TLTISGDIDKDEVKGLIEKYFGEIPGGRPVEKRDPMPVSLASTKKLYHEDNFARAPQL
                     TMVFPTSERYSRDSYALSFLGEILSNSKKAPLYVVLVKDKKLTSRVMARNMASELAGS
                     FMISVAANPGVNLSDVEKAIFEGLEKFEKEGFSEDDLTRIKAQYETRFYNSFESVLGK
                     SFQLAEYTMYTGDPGYYRKDIEAIQSVTMDDIRNVYNKYIKDRNFVQTSFVPRGELHL
                     IAEGSVNAGIVEEDVTRAAEIKSVEEEEEQIVKTPTSFDRSVKPVPGPDPAVSLPEVW
                     KSSLSNGTEILGIEHNELPLVEYAIIMDGGHISEDVSKAGLSGLVASMMNEGTKNRTP
                     EELEDAIGLLGASIRFSSGSENISVNVSTLKRNFEKTIALVEEMLLEPRWDEEQFELA
                     KDRIINNIERNRANPSYLASNSLNKLVFGDGNILAVDASGTRESVSSITIDDLKDYYG
                     RYFSPSGAKLVIAGNVTRKEVESAFAGLGKKWLPKEVVLPPVSIPGPPEKSAIYFVDV
                     PGAKQSIINIGAPSLPRISPDFYSAFVANYKLGGSFNGIFNLILREEKGFTYGARSAF
                     SGYKNFGTFSASSMVRTNSTLESVIIFKTEMEKYRENIPQEYVDFTKSALLKNNALNF
                     ETIRSLIAMLNNIASYNLPADYIKQEEAFLSSLTSRKLLELAKTYIDPARMYYVVVGD
                     AKTQLRELEKAGLGKPVLLN"
     gene            6342..6414
                     /locus_tag="PLZ75_06980"
     tRNA            6342..6414
                     /locus_tag="PLZ75_06980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6375..6377,aa:Gly,seq:gcc)
     gene            complement(6419..7162)
                     /locus_tag="PLZ75_06985"
     CDS             complement(6419..7162)
                     /locus_tag="PLZ75_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72175.1"
                     /translation="MRNISACLLIILSGISAFGRYAEITYPDKTESSRIHSPGKYREM
                     IRQNQTAFTAIIKTATRLFRDKDDLTSVILVIPTGKEVAVLGADSTYLRVVYEEAEGF
                     ILKRHAEISQTPLSSRTSIMPYATDDQAEIRSVPQPGSRTQTQPPEQSAAPSREAGSQ
                     VQTESQARRKERYNYLEKKYGSSMARKLIAGKIWKGMAREMIMDSWGKPLKINRIISG
                     NSTKEEWVYRNTWLYVKDDVLLTWGPVRK"
     gene            complement(7357..8577)
                     /locus_tag="PLZ75_06990"
     CDS             complement(7357..8577)
                     /locus_tag="PLZ75_06990"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOS72176.1"
                     /translation="MELLHHTLDNGIRLVHHRTNGITAHCGLIINTGSRDEREKEHGM
                     AHFVEHMLFKGTRKRKPYYIFSRLDNEGGELNAYTTKEETAIHASVLRDDFEKAVDVI
                     SDMAYNSVFPENEIEKEKEVIIEEINSCLDNPAELIYDDFEEMVFKGEALGRNILGTP
                     GHIRSITREALTAFIGRNYNTGETVFCSAGNIPDSKVIRLFQKYFGSIPEKNNRKNKS
                     RKQLYTPSTRIETRDTNQSHCIIGNIAWGYRDKRRIALLLLNNILGGQGLNSRLNMSL
                     REKRGLVYNIESGYNSYRDSGIVTIYFGTDSQNLERSIAIVNSELDKLRKTRLGTMQM
                     HTSKKQIKGYLARGYENYENLILSLGKSLLVFNKIDSYGEICSRIDSITSRELLDIAN
                     EVFNPDKLSTLIFK"
BASE COUNT         2058 a         2028 c         1967 g         2696 t
ORIGIN      
        1 aatataaacc ttttatgaag atgtttcagg ctattgttaa tattatcctt tgatttattt
       61 tttgtatttt attaaaatgc aaaaccctca tacataattt aatatgaggg tttgtctgta
      121 cccggaatgg gacttgaacc catacaggca ttactgccca caggatttta agtcctgcgt
      181 gtctaccaat tccaccatcc gggctggtat cgccgcgtgc tccgtttttc agcctgccgg
      241 gttctgccgg aaaaggatcg catgaagctt ccggcggaaa ctgggggcct tgtgattctg
      301 aagccggcaa tcatggttca aagataaaat aaaaatccct attggaacag cacaggtttt
      361 ccaaaaccga ctttttccag ctccttcagc agggtttttg catcgccgac aaccacgtaa
      421 tacattttca gggggtcgat atatttgcgg ataactgtat tcatctgctc tgcagtcagt
      481 ttccgaagat atgcttcctc ctgtttgatg taatctccgg gaagtccgtt aagtatcatg
      541 gtcctgagca tattgagaag ggcttcattg gtttcaaatc tcagggcatt ggctttcagg
      601 agggaatccc gggtgaattc aactgtttca gaagactgtc ctttgcggta ttccagcata
      661 atgtccctga acagccttac agagtcggga gtggcatcgg aacggacgga ggtccgggcg
      721 gaaaatgtac cgtattgccg gcctccctta aaggctgaat aggccccata ggtatatcct
      781 ttctcttcgc gcagtaccat gaaaagccgg gcacttgcac ctcctccaag catgaagttg
      841 gcagttacgg atttgtagaa atcttcatga ccgcggggca gggccagata acctgccttt
      901 attaccgatt gttttgctcc cggaacatcg acaaaatata atgatgcctt ttcgggggga
      961 gcaggaaaca ccggttccgg aatggcaaca tgttttttct tccatttttt cccaagttcc
     1021 tccagcgcct tttcaactct cggccggctg acgtccccga caatcatgaa ttcggccaca
     1081 gccggggata ctgccttgtc ataaaattcc ctgagatcat caattgttat tgattcaaca
     1141 ctcccggcag taccatcggc atggacagac agaatatttt ccctgccgaa gacaagattg
     1201 ttgaagaccc tgtctgccag ataattggga tcagacatgt ttcgtctgat ctcgtttatt
     1261 atccggattt tgccgagggc aaactgttcc tcatcccatc gcggctcaag gagcatttcc
     1321 tcaatcagtt caacggtttt ttcaaaattc cgggataatg aattcactga aaaagttata
     1381 tcttcgttat cgccgctgat ctcgaatgta gctccaagaa gctggattgc ttcttccagt
     1441 tcctccggcg ttttttttgc cgttccctca ttcagcatgg aagccatgaa ttttgcagta
     1501 cctgcttttt tcaggtcatc gagcatatgt cctcccctga ttgtcagtga ataggaaacc
     1561 atcggcagtt catgccgttc tataccgtgt attctcatac cattcgggaa aacagaattc
     1621 cacacttgcg gaagtctgat ttcaggatcc ggtccgggaa caggggcagc tgaacggtca
     1681 aatccggacg gggtttttat aatttcctcc tccatatcgg cttcaatatc aacctgtgca
     1741 gcccttgtta tgtcctcttc tgcaattccc gcattgacgg agccttcagc agccagatgc
     1801 agctgacctt tcggcacgaa actggttgcc acatagtttt tcccttcgat atatctgttg
     1861 tagacatccc acacatcttc agccgtaacg ctcttcatcc tgtcaagctc gcttattaga
     1921 taatcgggcg atccggccat cacgtcgtaa aatgccagca tgaatgattt gaacagaacg
     1981 ctgctgatgg tattgtaaaa ttctgtttca agcctggcct tgatcctttc aaggtctttc
     2041 tctgtaaatc cttctttccg gaatcttgaa aaggcctcaa aaataccttt ttcaacctca
     2101 ttcaggctgg tgtccggttt tgcagtaata ctgatcctta agtttccggt gagttccagg
     2161 gcctggttag cagccgtcac agccgatgtc agctgcttct ccctgaccag gacctggtgg
     2221 agcggggatt ttttcccctg gccgagaagc tgtgcaagaa cattcagtga atatgaatcc
     2281 ctggtgaaat tttcacatgt cggaaaaacc atgttgaact ggggtgcatt ggcaaaactg
     2341 tcttcatggt acagtttcct ggtttcattc agaataatgt ttgccggttc tggtttagtg
     2401 acaatattgc cggcgggtat ttccccgaaa tatttttcaa taaggaattt gacctcttca
     2461 gtttcgaaat cgcctgatac cacgagagtg gcattattcg gcacatagta tttccggtgg
     2521 aattccctta catcatcaat gccggcattt ttgatatctt ccatttcgcc gataacatcc
     2581 cagctgtacg ggtgtccggc ggggtagaga tttttcagaa tgacatggtc ggtgaaacca
     2641 tagggcatat tatccacata ttgtctcttt tcattctgga caacattctg ctgcaggctg
     2701 aatgagcttt gagtgacgct gttgatcata aaacccattc tgtcggattc gagccacatt
     2761 gccagttcaa gagcattttt cggaactact tcaaaataga cagtctggtc aaaggatgtg
     2821 cccccgttga gattacctcc tgcaccttca atatatctga agaactgatc ctcgcccaca
     2881 ttttccgaac gctggaacat catatgttca aaaagatgag caaagccggt tttccccggg
     2941 ctttcacggc cggatcccac atgatagaaa atgaccaggg aggttatagg attggatctg
     3001 tccttattga gaataactgt aagcccgttg gcgagcctgt atttttcatg ggggatggaa
     3061 aagccatcct ggtggttctc cggtttgccg gaaataaatg atctgagcgt catggattaa
     3121 tcaatgattt gttcattacg gttctgctga gggcagggaa acgcgtaagc gggccccggg
     3181 ttactccggt gcaggtccgg tactttactg cagtcccgtg ttgtggtgac tgaacaggat
     3241 aatggttagt catcatgcaa tactgcatca gttaagcaga accggttttc cgagtcctgc
     3301 tttttcaagc tccctcagct gggtttttgc atcacccaca acaacatagt acatccttgc
     3361 gggatcaata tatgtttttg ccagttcaag cagtttccgg cttgtaagtg agcttaaaaa
     3421 agcttcttcc tgttttatat aatctgcggg aagattatag gaagcaatat tgttcagcat
     3481 ggcaatcagg cttcttatcg tttcgaagtt cagggcattg tttttaagca atgcggattt
     3541 tgtaaaatca acatattcct gtggaatgtt ttccctgtat ttctccattt ccgttttgaa
     3601 gatgatgact gactcaaggg ttgagtttgt ccgtaccatt gatgatgccg agaaagtccc
     3661 gaaatttttg taacctgaaa aagctgaacg ggcgccatag gtgaatcctt tttcttccct
     3721 caggatcaga ttgaaaattc cgttaaacga gcctcccagt ttgtagtttg ccacaaatgc
     3781 ggaatagaag tccgggctta tccggggcag tgacggggct cctatattta ttatcgactg
     3841 ttttgctccc ggaacatcca caaaatatat tgcagatttt tccggggggc cgggtatgct
     3901 gacaggagga agaacgacct ctttgggcag ccatttcttc ccaagtcctg caaatgcaga
     3961 ttccacttcc tttctggtaa cattgccggc aatgaccagc ttggcacccg aaggggagaa
     4021 gtatctgccg taataatcct tcaggtcatc aatagtgatt gaactgactg attctctggt
     4081 tccggatgca tccactgcca gaatatttcc gtcgccaaaa accagcttgt tgaggctgtt
     4141 tgatgcaaga taggatggat ttgccctgtt cctttctatg ttgttgatta tcctgtcctt
     4201 tgccagttcg aactgttcct cgtcccagcg aggctcaaga agcatttcct caaccagggc
     4261 tattgtcttt tcaaagttcc gtttaagtgt acttacattt accgatatgt tttcagaacc
     4321 ggagctgaat cttattgatg cgcccagcag gccgattgca tcttcgagtt cttcgggtgt
     4381 cctgtttttt gttccctcat tcatcatgga cgcgacaagg cctgacagtc ctgctttgga
     4441 gacatcctcg ctgatatgtc cgccatccat gatgatggca tattctacca gcggcagttc
     4501 attgtgttca attcccagta tctctgtccc gtttgaaagg gatgatttcc agacctccgg
     4561 cagagacact gccggatcag gtccgggaac cggtttaacc gacctgtcaa atgaagtcgg
     4621 tgtttttact atttgttcct cttcctcttc gacacttttt atttccgcag cccgtgtgac
     4681 atcctcctcc acaataccgg cgttcacgga tccttccgca atcagatgca attctcccct
     4741 gggtacaaag ctggtctgaa caaaattcct gtccttaatg tatttgttgt atacatttct
     4801 gatgtcatcc atcgtaactg actgaatggc ttcaatatcc ttccggtagt atccgggatc
     4861 gcctgtatac attgtatatt cggccagctg gaaggacttg cccaggacac tctcgaagga
     4921 attgtagaaa cgtgtttcat actgggcctt gattcttgta aggtcatcct cgctgaaacc
     4981 ctctttctca aatttctcca ggccctcaaa aattgctttc tcaacatcgc tgaggttcac
     5041 acccggattg gctgcaacgg aaatcataaa ggaaccggcc agttcacttg ccatattcct
     5101 ggccatcacc cttgaagtca gctttttgtc tttaaccaga acaacataga gaggtgcctt
     5161 tttactgttg gacagtattt ccccgagaaa actgagggca taggaatctc ttgaatatct
     5221 ttcgcttgtg gggaatacca tggtaagctg cggggccctg gcaaaattat cttcatggta
     5281 aagctttttt gttgaagcaa gtgataccgg catgggatct cttttttcaa caggcctgcc
     5341 cccgggtatt tcaccgaaat atttttcaat aagtccttta acttcatcct tgtctatgtc
     5401 tcccgatatg gtaagcgttg cattgttggg aacgtagtat ttcccgtgga atgcctttac
     5461 atcttcaatt gttgcatttg taaggtcttc catcaggccg atcacagtcc agctgtacgg
     5521 atggccttcg gggtaaagat tctttgcaat gatttcctga ttgaatccgt atgcagcatt
     5581 atccacgctc tggcgttttt cattctggac aacattctgc tgattggcga gggatgattt
     5641 tgtgacggtg tttgcaagat aacccattct gtctgattca agccacagca ccagttccag
     5701 ggcatttttg ggaaccactt cataataatt tgtacgatcc tggctcgtgc ttccgttcaa
     5761 tgttcctcct gcgccctgta tttcccggaa cagctggtct tcgccgacat tttcggacct
     5821 ctggaacatc atgtgttcaa agaggtgggc aaaaccggtt ttcccgggtt cttcccggct
     5881 tgatccgaca tgatagtaaa ttgcgactgc ggcaatcgga tcggatttgt caacattgag
     5941 aaccacggtc agaccattcg ggagagtgta tttttcaaac gggatggata attccgtttt
     6001 tctgtttcct gaattgcatg aaaacataat tgcaactaaa aaaattaaag ggagcgaaaa
     6061 tagctttttc atatcagaat ttttattgtg ccggtaagaa tctttccggc tgttaaactg
     6121 atgaaccaca cgaaaagttc aaagatagga gttaaaaggc agaaggtttg aatcattatg
     6181 aaaatagtaa tcaaaaaaca ctgtttcttc agctttgttc cccgctattt gtcagctgct
     6241 ttggtgttgc cgccatggcg aagtgcatga aacgggaata aaaatcatta tcctgctgat
     6301 tttgcatata aaaaaaagtt cttatttttg cagctgaatc tgcgggagta gctcagttgg
     6361 tagagcataa ccttgccaag gttagggtcg cgggttcgag tcccgtctcc cgctcaagct
     6421 atttcctgac cggtccccag gtcaacagaa catcatcttt tacataaagc caggtgtttc
     6481 tataaaccca ctcttccttt gttgaatttc ctgatattat cctgtttatt ttcaatggct
     6541 taccccagct gtccatgatc atttcccgcg ccattccctt ccagatcttg ccggcaatga
     6601 gctttctggc catactgctt ccatattttt tttcaagata attatatctt tctttccggc
     6661 gggcctgcga ttctgtctgc acttgtgatc ctgcctcccg ggacggtgcc gcggattgtt
     6721 caggaggctg tgtctgagtc cgggatcccg gctgaggcac agaccggatt tctgcctggt
     6781 catccgttgc atacggcatg atggaggttc tggatgaaag tggagtttga ctgatctctg
     6841 catgtctttt taaaatgaat ccttcggctt cttcatatac aaccctgaga tatgtgctgt
     6901 cagcacccag tacagcgact tcttttccgg tgggaatgac aagtatgacg gacgtaaggt
     6961 catccttgtc acggaaaagt cgtgtggcgg tttttattat tgcggtaaat gcagtctggt
     7021 tttgccggat catttccctg tactttccgg ggctgtgaat cctgctgctt tcagttttat
     7081 ccggataagt gatttccgca taccggccaa atgcggaaat gcctgaaaga attataagaa
     7141 ggcaggctga tatattcctc atgatctttt tatcctgaga gttcaaaaat tgtaccacat
     7201 tgttaacata tgcactggaa gggatgtccg ggtctgtcgt aggccctaac ccggtccgtt
     7261 tccgtatctg ccgcatttca ggtgtattta aaaccggggg ctcatttccg gccccggcag
     7321 acggtctgct tgtttttaag gattccggca gggattctat ttgaagatca gagtggaaag
     7381 tttatccggg ttaaaaactt cgtttgctat gtcaagtaat tcccgggagg tgatactgtc
     7441 aatcctggaa cagatttccc cgtagctgtc aattttgtta aataccagga ggcttttgcc
     7501 aagactgagt atcaggtttt cgtagttttc ataccctctt gcaagatagc cttttatctg
     7561 ctttttcgag gtatgcatct gcattgtccc gagcctggtc tttctgagtt tgtcaagttc
     7621 ggaattaacg atggctatgc ttctttccag attttgactg tcggtgccga aataaattgt
     7681 aacaataccg gaatccctgt aggaattata gccggattcg atgttataga caagccctcg
     7741 tttttcgcgg agtgacatgt tgagccggga gttgaggccc tgtccgccaa gaatgttatt
     7801 gagtaggagc agggcgatcc tgcgcttgtc tctgtatccc caggcaatat ttccgattat
     7861 gcagtggctc tggttcgtgt ctcttgtttc gattctggtt gaaggagtat agagttgttt
     7921 ccggcttttg tttttcctgt tgtttttttc aggaatgctt ccgaagtatt tctggaagag
     7981 gcgtatgact ttactgtcag gaatgttgcc tgctgaacag aagacagttt cgccggtgtt
     8041 gtagtttctc ccgataaagg ctgtcagggc ttccctggta attgatctga tgtgtccggg
     8101 tgttccaagt atgtttctgc caagagcttc ccctttaaac accatttcct caaaatcgtc
     8161 ataaatgagt tctgccggat tatccaggca ggagtttatc tcttcaatga tcacctcttt
     8221 ttccttttcg atctcatttt cggggaaaac cgagttgtat gccatatcac tgattacgtc
     8281 aacggccttt tcaaaatcat ccctgagaac cgatgcgtgt atggcagttt cttcctttgt
     8341 ggtataggca ttcagttctc ctccctcatt gtcgagacgg ctgaagatat aatatggttt
     8401 tcgtttccgg gttcccttga acagcatatg ctccacaaaa tgagccattc cgtgttcttt
     8461 ttccctttca tcacgggatc ccgtattgat tataagaccg caatgggcgg ttattccgtt
     8521 tgttctgtgg tgaacaagcc ttattccgtt gtcaagtgta tgatgaagaa gctccatcaa
     8581 atattttttc caaagctgca aatgtaacac aagactgaaa cgaatccaaa aagatttttt
     8641 tagtttaaag ttttgcttac tcggatttga gatatatatt tgcagtcctg tcagcaggta
     8701 ccatagctca gtcggtagag caacggactg aaaatccgtg tgtccctgg
//
LOCUS       DAOSPX010000113         8558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7859_length_8558_cov_4.352111, whole genome shotgun sequence.
ACCESSION   DAOSPX010000113 DAOSPX010000000
VERSION     DAOSPX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7859_length_8558_cov_4.352111"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLZ75_06995"
     CDS             complement(<1..477)
                     /locus_tag="PLZ75_06995"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158407.1"
                     /note="catalyzes the phosphorylation of ribulose to
                     ribulose 5-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOS72177.1"
                     /translation="MAKKYVIGVDYGTDSVRSVVVDTTSGNIAGTSVFEYPRWKKGLY
                     CDPSANRFRQHPLDYTEGLERSVRGALRGLGRQITDNIAGITVDTTGSTPVAVDRNGT
                     PLSLVPGFEDDPDAMFILWKDHTAVDEAAEINELARSWGGTDYTKYEGGIYSSEWFW"
     gene            complement(541..1236)
                     /gene="araD"
                     /locus_tag="PLZ75_07000"
     CDS             complement(541..1236)
                     /gene="araD"
                     /locus_tag="PLZ75_07000"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HOS72178.1"
                     /translation="MLEKLKARVYRANTELVKSGLVIHTWGNASARDRKTGLIVIKPS
                     GISYDKMKAGDMVVLDPDGNIVEGRYKPSTDAPTHLFLYKDWDLINGIVHTHSTYATS
                     WAQAGRNIPAFGTTHADHFHGEVPCTRRLTAEETENDYEINTGKVMAERLSGLDPLAV
                     PSVLVNCHGPFSWGRDPEEAVYNAIALEEIARMAFYTVLLGNTGPADVHLLNKHFERK
                     HGKNAYYGQKKKK"
     gene            complement(1243..2736)
                     /gene="araA"
                     /locus_tag="PLZ75_07005"
     CDS             complement(1243..2736)
                     /gene="araA"
                     /locus_tag="PLZ75_07005"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOS72179.1"
                     /translation="MNELRKKEIWFITGSQHLYGPDVLRQVEADSTEIVASLNSSEII
                     SRKIVFRRVLTTPESILETCLEANSTGSCIGLIVWMHTFSPAKMWINGLRVLDKPLLH
                     FHTQLNRDIPWDSIDMDFMNLNQSAHGDREFGFITARMRLGRKVVVGHYRDDITLKRI
                     AVWIRAAAAYDDARNMKIARFGDNMRDVAVTEGNKVSAQIRMGYSVYGYGIGDLVKAV
                     NEVTGEEIRSLLDEYSSEYKMTREVASSGSLREAARIEAGMEKFLLGGGFRAFTTTFE
                     DLHGLKQLPGIAVQRLMARGYGFGAEGDWKTAALVRSVKVMADGLKGGTSFMEDYTYH
                     FDPAGMRVLGAHMLEVCPTIAEDKPVVEIHQLSIGGKEDPARLVFRTAPGNGINVSVI
                     DLGNRFRMIVNDVKVVKCPDMPLLPVASVLWEPAPDLRTGAAAWILAGGAHHTAFSTA
                     IDAEYLEDFAEMQDIEFIRIDEKCDLHRLKNELKWNEVYYHISNGIK"
     gene            complement(2895..4547)
                     /locus_tag="PLZ75_07010"
     CDS             complement(2895..4547)
                     /locus_tag="PLZ75_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOS72180.1"
                     /translation="MAILDWIVLGLFCLGLVGIILWVLKQREETSTDYFLAGRDATWI
                     AIGASIFASNIGSEHLVGLAGAGASSGMAMAHWEMHGWIILILGWFFVPFYARSGVFT
                     MPEFLEKRYNKGSRSVLSIISLVSYVFTKVAVTVYAGGVVFKQVFGIESMFGIDFFWI
                     SAVGLVILTGIYTTLGGMKAVLYTSVLQTPVLLIGSIVVLIIGLIKLGGWSEMMEICR
                     AVPVNDYGNNMTQLIRSGQDPEFPWTGVILGSAIIGFWYWCTDQYIVQRVLSGRNQTE
                     SRRGTILGASFKLLPVFIFLIPGMIAYGLNVKGIITLPDSDSAFAVLVKELLPVGFKG
                     LVVGGLLAALMSSLASLFNSSATLFTVDFYQKYRPEASEAELVKVGKIATMVVVVLGV
                     LWIPVMKGLGKVLYAYLQDVQSLLAPGIAAVFLLGIASKKTTPKAGFTGLLTGFILGM
                     TRLAFKVFESGISPDSFLYRIFLKPNWLHYEIVIFFIIIILMILVSMVTEKADPASIR
                     SLYVGSATAEEKAITRASWNNWDLFFSGLIIVVIIGFYIYFW"
     gene            complement(4583..5383)
                     /locus_tag="PLZ75_07015"
     CDS             complement(4583..5383)
                     /locus_tag="PLZ75_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOS72181.1"
                     /translation="MDKGYFKSDTYVTSEDSLGSWRKIYRHNRLYFTLKFINIIFRTR
                     REARKGIYDDKAWEESSFYIFRFIEKSGGRFNISGMSNISVTEEPVLFIANHMSTLET
                     MILPCIISPLRKVTFVVKENLVSHPLFGDVMKSRDPIIVGRTDPRKDLEAVMNGGMEL
                     LSRGISIVIFPQSTRNIIFNPSEFNSLGVKLAKKAGVKVVPVALKTDFWGNGKVVKEL
                     GPLDSMKTICFRFGEAFDVKGSGKDENQKIIDFIQSSLLEWQEKDSRK"
     gene            5616..7136
                     /locus_tag="PLZ75_07020"
     CDS             5616..7136
                     /locus_tag="PLZ75_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOS72182.1"
                     /translation="MKLPLLDSIVFFTLTLGNIIFGISFYFKNRTTDQFTAAGGKLPA
                     WVVGMSIFATFVSSISFLGLPGKAYATNWNAFVFSLSIPFATIMTIRFFIPLYRSIGN
                     ISAYYYLEQRFGAWARIYASACYILTQLMRTGAILMLLALPINALFGWNVKTVIILTG
                     LMVVIYTMLGGIKAVIWTDTVQGIILITGALICSVILTFSMPEGPGQLFRIAVENNKF
                     SLGSLGSSLSQSTFWVVLVYGLFINLQNYGIDQNYIQRYMTTGSEREAKASAWFGSML
                     YIPVSLLFFYIGTALFSYYTAMPELLPDELHATGAADRVFPYFIATGLPAGITGLLIA
                     SIFAAGMSTVSTSLNSTATIVMNDYYKRYLNRNAGEKQCMRVLYTASFLTGVFGILIA
                     LALVGVESALDAWWSLASIFSGGMLGLFLLGYFSRKAKKADAVTGVVCGVLVIIWMSL
                     SPLYFTEGKSLAFRSPFHTNLTIVFGTMTIFLAGFLVSRFISLRIPHNRENNKINT"
     gene            7240..8301
                     /locus_tag="PLZ75_07025"
     CDS             7240..8301
                     /locus_tag="PLZ75_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOS72183.1"
                     /translation="MKRVIPVTLLTATVLITGISAQFSGSGCIIKSEFIYQPADVPFP
                     SCHASTLVQNGKGLMAAWFGGTEERNPDVGIWTSSFVKGRWTRPVEVANGVQHKDKRY
                     PCWNPVLYNTGKKILLFFKVGPSPSTWWGEMMFSDNRGRSWSVPRRLPEDILGPVKNK
                     PVLLDNGELLCPSSTEDEGWRLHMEFTSDEGLTWERTSALNERTTGAIQPSILVHPGG
                     KLQLLCRSTVSRILSAWSEDRGRSWSRLEALDLPNNNSGIDAVTLLDGRHILVYNHLE
                     KGRHALHVAVSDDGISWKAAAILENDRQGSEFSYPAVIQTSDGLVHITYTWNRKQIKH
                     VIIDPAAIQATSFHNNEWP"
     gene            complement(8348..>8558)
                     /locus_tag="PLZ75_07030"
     CDS             complement(8348..>8558)
                     /locus_tag="PLZ75_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxo-5-alpha-steroid 4-dehydrogenase"
                     /protein_id="HOS72184.1"
                     /translation="FDYISSPHYFGEIIEWGGWALMTWSLPGFAFFVFTFANLFPRAI
                     NTHRWYRGKFPAYPPGRKAVIPYLL"
BASE COUNT         2225 a         2183 c         1840 g         2310 t
ORIGIN      
        1 ccagaaccat tcggaggaat agattccccc ctcatatttc gtataatccg taccacccca
       61 cgatctggca agttcattta tttccgccgc ttcatccacc gcggtgtgat ctttccatag
      121 aatgaacatg gcatcaggat catcctcaaa gcccggaaca agcgataaag gcgtaccgtt
      181 cctgtcaacg gcaaccggcg tggatccggt tgtatcaaca gttatacccg ctatattatc
      241 agtgatctgc ctgcctaaac ccctgagggc gcccctgact gatctttcaa gcccttccgt
      301 gtaatcgagg ggatgctgac ggaaacggtt ggctgaagga tcacaataga gtcccttctt
      361 ccacctggga tattcaaaaa ccgaggtccc tgcaatattg ccgctggtcg tgtcaacaac
      421 aacagaacgg accgaatccg tgccgtaatc aacgcctata acatactttt ttgccatagc
      481 tgtcttttct atttatcgtt tttgttctta atatattttt tcaataccct accgcttcgc
      541 tcacttcttt ttcttttgtc cgtaatatgc atttttgcca tgttttcttt caaagtgctt
      601 attaagaagg tgaacatcag caggcccggt atttccaaga agaaccgtat aaaatgccat
      661 tctggcgatc tcttccaggg caattgcatt atatacggct tcttccggat cccgtcccca
      721 gctgaatggg ccatgacagt ttactagaac tgatggtaca gccaggggat caaggcctga
      781 gaggcgttct gccatcacct tccctgtgtt gatctcgtaa tcgttttctg tttcttctgc
      841 ggtaagcctg cgtgtacacg gaacctcacc gtgaaaatga tctgcatggg tggttccgaa
      901 agcgggaata ttcctgccgg cctgagccca cgatgtggca tatgttgaat gtgtatggac
      961 tatcccgttt attaaatccc aatctttata cagaaagaga tgggtcgggg catcagtgga
     1021 gggcttgtat ctcccctcaa ctatattacc atccggatcc agcacaacca tatcgccggc
     1081 tttcatttta tcatagctta ttccggaagg tttgatgact atcagccctg ttttcctgtc
     1141 acgtgcactt gcatttcccc atgtatgaat aaccaggccg ctttttacga gttccgtatt
     1201 tgccctgtaa acccttgctt taagtttttc aagcatgtcc agctatttaa taccgtttga
     1261 gatatgataa taaacctcat tccatttcag ttcatttttc agcctgtgca gatcacattt
     1321 ttcatctatc ctgatgaatt caatatcctg catttcagca aaatcctcca ggtattccgc
     1381 gtcaatggca gtactgaacg cagtatgatg tgctcctccg gcaagtatcc atgccgcagc
     1441 cccggtcctg agatcgggcg ccggctccca cagaacactg gcaacaggca gcaggggcat
     1501 gtcggggcat tttaccacct tgacatcatt aacaatcatt ctgaatctgt tacccaggtc
     1561 aataaccgaa acatttattc catttcccgg tgcagtcctg aaaaccagtc tggcaggatc
     1621 ctccttgccg cctatggaca gctggtgtat ctccacaacc ggtttgtcct ctgcaatcgt
     1681 cggacagact tccagcatat gtgccccgag aaccctcatt cccgcggggt caaaatgata
     1741 ggtatagtct tccatgaatg aagttccccc tttcaggccg tcagccatta ctttcaccga
     1801 gcgtaccagg gctgcggttt tccagtcgcc ttccgcaccg aatccgtaac ccctggccat
     1861 aagacgctga accgctatac ccggtaactg tttcagtccg tgaagatcct cgaaagtggt
     1921 tgtgaaagcc ctgaatcctc ccccaagaag aaatttctcc atcccggcct caatcctggc
     1981 agcttccctt aaagacccgg aggacgcaac ctctcttgtc attttatatt cagaggaata
     2041 ctcatccagc agactccgga tttcttctcc ggtaacttca tttacagcct taacaagatc
     2101 tccgataccg tatccgtaaa ccgaatatcc catccttatc tgagcactga ccttgttccc
     2161 ctccgtaacg gcaacatcac gcatattatc gccaaaacgg gcaattttca tgttccgggc
     2221 atcatcgtat gcagcagctg cccttatcca gacagctatt ctttttagcg taatgtcatc
     2281 cctgtaatgg cccacgacaa ccttcctgcc cagccgcatc ctggccgtaa tgaatccgaa
     2341 ctcccggtca ccgtgcgccg actggttcag gttcatgaaa tccatatcga tggaatccca
     2401 tggtatatcc ctgtttaact gtgtatgaaa atgcagaagg ggcttatcca gaaccctgag
     2461 tccgtttatc cacatctttg ccggcgagaa ggtatgcatc catactatca gaccgataca
     2521 gctgccggtg ctgtttgctt ccaggcatgt ttcaagaatt gattccgggg tggtgagcac
     2581 cctcctgaag actatttttc gtgaaataat ctctgaactg ttcagcgatg caactatttc
     2641 cgtggaatca gcttccacct gtctgagaac gtcaggtccg tataagtgct ggcttcccgt
     2701 gatgaaccag atttcctttt ttcttaattc attcatgtta ctggcttgaa ataagtaatt
     2761 atgtttttaa ccggtctgtc ctttcctccc ggcagatgaa gtttctccct gctgcgcttt
     2821 atgaactgca tctgcctgac atggtatgtc atgttaaata atgaaccgct tccggcagac
     2881 ctttacggat cagcttacca gaaatagata tagaatccga tgataaccac gattatcagt
     2941 ccggagaaaa agagatccca gttattccag cttgccctgg ttattgcctt ttcttcagct
     3001 gtggccgaac caacatacag actccgtatg gatgccggat ctgccttttc tgtcaccatg
     3061 ctgaccagta tcatgagaat gattataatg aaaaaaatca caatctcgta atgcagccag
     3121 ttgggtttga gaaaaatcct gtacaagaaa ctgtcagggc ttatccccga ttcaaacact
     3181 ttgaatgcaa gtcttgtcat tccaaggata aaacctgtaa gcaatcctgt aaaacctgcc
     3241 ttgggggtag tcttttttga cgcaattccc agcaggaaca cagcagcaat tccgggagca
     3301 aggagtgact gaacatcctg gaggtaggcg tagagtacct tcccgagtcc cttcatgacg
     3361 gggatccaga gaactcccag aaccacaacc accatcgttg caatcttccc gacttttacc
     3421 agttccgctt cactggcctc cggcctgtac ttctgataga agtccacggt aaacagggtg
     3481 gctgatgaat tgaacaggga ggcaagtgag ctcatcaggg cggcaagaag accgcctaca
     3541 accagtcctt tgaatccgac cggaagcagt tcttttacca gaacggcaaa agcggaatca
     3601 gaatcaggaa gggtgatgat tcccttaaca ttcagtccgt aggctatcat tccgggaatc
     3661 aggaatatga atacgggtag aagcttgaaa gacgctccaa ggatggttcc ccttcttgat
     3721 tcggtctggt tgcggcccga caggaccctc tgaacaatgt actgatccgt acaccagtac
     3781 cagaatccta ttattgcgga tccgagtata acgccggtcc aggggaactc aggatcctgc
     3841 cccgacctta tcagctgagt catattgttt ccgtaatcat tcaccggcac ggcccgacag
     3901 atttccatca tttcggacca tcctccaagt tttatcagac cgataataag caccactatt
     3961 gatccgataa gcagaaccgg agtctgcaga accgatgtat acaaaacggc cttcattccg
     4021 ccgagggttg tataaatccc tgtcagaata acaaggccta cagcactgat ccagaagaaa
     4081 tcgataccga acattgattc aattccgaag acctgcttga aaacaactcc ccctgcatag
     4141 acagtaacgg ctaccttggt gaacacatag cttaccaggg atatgatgga caaaacactt
     4201 cgtgatccct tgttatatct tttttcaagg aattcgggca tggtgaacac cccgctcctg
     4261 gcatagaacg gtacgaagaa ccatcccaga atcagaataa tccatccgtg catttcccag
     4321 tgagccattg ccattccgct tgaagctccc gcacctgcca gaccaacaag atgttccgat
     4381 cctatattgg atgcgaagat tgaagcccct atagcaatcc atgttgcatc acggcccgcc
     4441 agaaaatagt ctgttgatgt ttcctccctt tgtttcagta cccacaggat gattccaaca
     4501 agaccaagac aaaagagtcc cagtacgatc cagtccaata ttgccatgtc agataattta
     4561 aaattatttc ataaaattca tttcactttc tgctgtcttt ttcctgccat tccagcaggg
     4621 aagactgaat gaaatcaata atcttctgat tttcatcctt gccgctgcct ttaacatcaa
     4681 aggcctcccc gaacctgaaa cagatggttt tcatactgtc gagcggaccc agttccttta
     4741 ccacttttcc attcccccag aaatcggttt tcaaagctac cggaacaact ttcactcctg
     4801 ccttcttagc cagtttaaca ccaagggaat taaattcgga aggattaaaa atgatattcc
     4861 ttgtactctg gggaaaaata actattgaaa ttccccttga cagtaattcc atcccgccgt
     4921 tcatcacagc ctcgagatct ttcctgggat cggtgcgtcc gacaataatc gggtccctcg
     4981 atttcatcac atcaccaaaa agaggatggc tgacgagatt ttccttaacc acgaaagtga
     5041 ctttcctcag aggcgaaatg atacatggaa gtatcattgt ttcaagagtg ctcatgtggt
     5101 tggcaatgaa caacacgggt tcttccgtta cggaaatgtt actcatcccg gaaatgttga
     5161 acctgcctcc cgatttttct atgaacctga atatgtaaaa ggaagattct tcccaggcct
     5221 tgtcatcata tatccctttt ctggcctccc tcctggtcct gaaaataata tttataaatt
     5281 taagcgtgaa atataatctg ttgtggcggt atattttccg ccatgatccg aggctgtctt
     5341 cggaagtaac atatgtatcc gacttaaaat atcccttgtc cataaaccat actattctgt
     5401 tccgggctta tctttttgta gaaattactg ccattgccgt attgtttgtt atgtcatatc
     5461 ctgatttctg ccggtaccgg tactgtcagg gaagttatgg tttttcattt caatactggc
     5521 atgggtttaa aatcgatgtc tgtttacaaa actaagatat aaattgtaag tttaagcgca
     5581 aataaactac gtatctttat taatatttat ttccgatgaa gcttcctctg cttgattcca
     5641 tagtattttt cactcttacc ctggggaaca ttatttttgg aatcagtttc tatttcaaaa
     5701 acaggacaac agatcagttc acggctgcgg gcggcaagct tcctgcctgg gttgtcggaa
     5761 tgtcaatctt tgcaactttt gtaagcagca taagtttcct cggactaccc ggtaaggcct
     5821 atgcaacaaa ctggaatgct tttgttttca gtctttcaat tccctttgcc actataatga
     5881 caatcaggtt tttcattccc ctctacagga gtatcggaaa catatcagcc tattattatc
     5941 ttgaacaacg gttcggggcc tgggcaagaa tctatgcttc agcatgctat atacttaccc
     6001 agctgatgcg caccggggcc atactgatgc ttcttgcact tcctatcaat gcacttttcg
     6061 gatggaatgt aaaaaccgtg ataatcctta cgggcctgat ggtggtgatc tataccatgc
     6121 tggggggaat aaaggccgtc atatggactg atactgtaca gggcatcatt ctcatcaccg
     6181 gtgccctcat atgttccgtc atactgacct tttccatgcc agaggggccg ggtcagcttt
     6241 tcagaattgc agtggaaaac aacaaattca gtctcggaag ccttggatca agcctttccc
     6301 agtcaacttt ctgggtggtg ctggtctacg gactttttat caaccttcag aactacggca
     6361 ttgatcagaa ctatatccag cgttatatga caacgggaag cgaaagggaa gcaaaggcct
     6421 ccgcatggtt cggatcaatg ctctacattc ctgtttccct gctgttcttt tatattggaa
     6481 cagctctttt ttcctattac acggccatgc ctgaactgct gcctgatgaa ctccacgcta
     6541 ccggggcggc agacagggtc tttccctatt tcattgccac aggtctgccg gcaggaataa
     6601 ccggactgct gatagcatcc atctttgctg ccggaatgag cactgtctca accagcctca
     6661 acagtacggc aaccatcgta atgaatgatt attacaagcg ctatctgaac cggaatgccg
     6721 gcgaaaaaca atgcatgaga gttctttata cagcctcatt cctgacaggt gtttttggaa
     6781 tcctgatcgc ccttgccctg gtgggagttg aaagtgccct ggatgcatgg tggtctctgg
     6841 cttcaatctt cagcggcgga atgctcggtt tgttccttct ggggtatttc tcgcggaaag
     6901 caaaaaaggc agatgcagta acgggtgttg tatgcggagt cctggtaata atctggatga
     6961 gcctctcccc tctttatttc accgaaggaa aaagtctggc attcaggagt ccctttcata
     7021 ctaacctgac gatagtattc ggaacaatga caatattcct ggcgggcttc ctggtttccc
     7081 gcttcatcag cctcaggatc cctcacaaca gagaaaacaa caaaatcaac acctgaccca
     7141 aatcgacacc agaaccaaat caacaccaga accaaatcaa caccagaacc aaatcgacac
     7201 cagaatcaaa tcaacacctg aaccaaatga atatagctta tgaaaagagt tattccagtc
     7261 acgttgttaa cggcaaccgt tttaatcaca ggtatcagtg cccagttcag cggcagcgga
     7321 tgtatcatta aatcggaatt catctatcag cctgctgatg ttccctttcc ttcctgtcat
     7381 gcctcaacgc ttgttcagaa cggaaagggc cttatggcag catggttcgg aggaaccgag
     7441 gaaaggaatc ctgatgtggg aatctggacc agcagtttcg tgaaaggcag atggaccagg
     7501 cccgttgagg ttgcaaacgg cgttcagcat aaggataaac gttatccgtg ctggaacccg
     7561 gtactctaca atacaggaaa aaagattctt ctgtttttca aggtaggacc cagtccttca
     7621 acatggtggg gcgagatgat gttctcggat aacaggggca ggtcatggtc ggtcccccgg
     7681 agattgccgg aagatattct ggggcctgta aaaaacaagc ctgtcctcct tgacaacggg
     7741 gagctgctgt gtccttccag cacggaagac gagggctgga gactgcacat ggaatttact
     7801 tccgatgaag gacttacatg ggagcggacc tcagccctga atgaaaggac gacgggggct
     7861 attcagccct ccatactggt tcatccggga ggaaagctgc agctgctgtg ccgcagtacc
     7921 gtatcaagga tactcagcgc ctggtcggag gacaggggcc gcagctggag cagactggaa
     7981 gccctggatc tgccgaataa caattcgggt atagatgcgg tgacactcct tgacggccgt
     8041 catatccttg tctacaacca tcttgaaaag ggtcgccatg cccttcatgt agctgtttcg
     8101 gatgatggta tcagctggaa ggcagctgca attctggaaa atgacaggca gggaagtgaa
     8161 ttttcatatc cggcagtgat acagaccagt gacggcctgg tacatatcac ctatacctgg
     8221 aatcgtaaac agataaagca tgtgatcatt gatccggctg ccatacaggc aacatctttc
     8281 cacaataacg aatggccctg agccctgttg tccgggtatt gtgacagcaa tccgccacga
     8341 atgataccta tagtaaataa ggaatcactg cttttctgcc gggcggataa gccgggaatt
     8401 ttcccctgta ccatctgtgt gtgttaatgg ctctggggaa aagattggca aaggtgaaaa
     8461 caaaaaatgc aaatccggga agggaccatg tcatcagggc ccatcccccc cattcaatta
     8521 tttcaccgaa ataatgcggt gaggaaatgt aatcgaaa
//
LOCUS       DAOSPX010000114         8525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7888_length_8525_cov_3.761157, whole genome shotgun sequence.
ACCESSION   DAOSPX010000114 DAOSPX010000000
VERSION     DAOSPX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7888_length_8525_cov_3.761157"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2454)
                     /locus_tag="PLZ75_07035"
     CDS             complement(<1..2454)
                     /locus_tag="PLZ75_07035"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOS72185.1"
                     /translation="MKKYKLINDTTGWVVFAIAAAAYLLTMEPTTSFWDCGEFIASAY
                     KMEIGHPPGSQVFMVLARFFTLFAGGNVAKVAVMVNAMSALASAFTILFLFWTITHLA
                     RRIMVRNGNEASLSDIIAIMAAGVVGSLAFTFSDSFWFSAVEGEVYATSSFFTAASFW
                     AILKWEDEAEEKHAGRWIILVAYLMGLSIGVHLLNLLALPAIVLVYYFKKSEFTWKGL
                     VLSLGISFIILAILMWGIIPGIVRISSRLDLFFVNKLNLPVNSGMIFHGILLVVLFTA
                     AIYYSLNSADRVKNSIIAAAAVFLTGAWVISGSVLLNLVVFLVLAGIIWYIASGSRVT
                     LNTVITALAVVLIGYSSSAIIVIRSSANPPLNENNPSNPFNLLYYFNREQYGSRPLIR
                     GAYYNAPVLDYKTGKPAYALEDGKYIITHHELERVFDERFYTLFPRMWSDQPDHEEMY
                     RQWGGTKGIPLQVTGQSGEKSIIRKPRFSENLRFMFTYQYGYMYFRYFMWNFAGKQND
                     TQGTGGAVNGNWISGFKFLDEPRVGTSDLPPEQRNDTSRNKYYLLPFFLGLIGLFYHL
                     NRDIKNWWIVMLLFIMTGIAIVFYLNQYPNQPRERDYAYVGSFYFYAVWIGFGVLALF
                     EAFSRLAGKKVAAPLAGILCLAAVPALMAVENWDDHDRSGRYLARDVAFNYLESCAPN
                     AILFTNGDNDTFPLWYAQEVEGRRTDVRVCNLMLLNTDWYIDQMKRKTYESDPLPVTF
                     SEKKYYDGINNQVFIVEKTKEAVDIGTIIDWIKSDNPATKVRVSATELLDIIPSRTIR
                     IPVDARKVIESGTVSPEDSA"
     gene            2578..5607
                     /locus_tag="PLZ75_07040"
     CDS             2578..5607
                     /locus_tag="PLZ75_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72186.1"
                     /translation="MKIKRHIVSALIFQISLLLSAQYYNTGQDPAGLKWLQIKTGPFR
                     IIFPEEYDAAGKEFARVLEDAHKAVSYPGFAGRYRLPVIIHNYTTQSNGYVAWAPRRM
                     EIYPTPEQNSIPLDPFSQLAYHELTHVYQMDTFSRGFSRAMIMFFGQQFPGVVASLVP
                     QWFLEGDAVFNESFFTPSGRGRTASFQKYLKAVSLERGKMYKYDQLVNGSYRYFIPDH
                     YESGYQIIAWSKLRYDRDVWERALRLTANAPFLANPVNLSLRHSIGKTKKMLADETFD
                     TLTVIWKDEIELHNPAEYEMISPPGGNKYISYHSPVRISENRYAAVRTSLDEPPAIVI
                     VNSTDRTERKIQSPGSMYPYVISSGRGFLAWVETRPDPRWANRNYSVIRLMDTGNGVT
                     RQLSRKTRYMSAAISPDSELIAASENTAGNKNNLVLIDRRSGKIIKSVPVPGNASLQK
                     PRWSDDGRKISMISLTAEGEGIISFSLQDMKWEKLIPESPVDYQSAVIRNDSLFFVSS
                     ESGTENVYVLTPDKRTIRITSVFFGATDPLPDGKKIVFSNYSLTGNKLCMTGITAGEE
                     DAGSERTAGMEAGYERTAGQDTVYKSGSGTAFLIDRIKTPGERTETPGERTETPEKER
                     EETGKQPETREYVTERYNKWRHLFNIHSWMPFYADIEEIKADPLAVRPGFTVFSQNHL
                     STLTASAGYEYSDRLHKLHSGIRWEGWYPVYDAGIHYGDNPSVLKSKSEDPDPLSVNP
                     GIHFTNSLSLPLRFSSGKYYQFLQPSLSLSFRNNYIYVKEDALYDYGQTMTAARLYFY
                     NARRSSVRDIYPIYAQVIDLYYSSYPWDRDFYAPLASVQTAFFFPGLFPNNSLRIRLE
                     GEQQGSAEFLRTNRLHFPRGYRNIISEKIFFASADYRAPLACPDLSIGSLLYLKRLRA
                     GIFFDYARGTNNYYLQHRSSGGFRVDHVHRYAESFTSTGAELIADFHVLRLPYMISAG
                     VQAAWSADFASPVLEAIFSIDIHGMSIGRSGPGSIRL"
     gene            complement(5597..7015)
                     /locus_tag="PLZ75_07045"
     CDS             complement(5597..7015)
                     /locus_tag="PLZ75_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOS72187.1"
                     /translation="MTAEEFRKHAHELVEWITDYLENKEKYPVKPRVKPGEIFSRLPD
                     GAPQEPEPFEKIQKDFDEIIMPGMTHWQSPNFFAYFPANSSPPSVLAEMLTATLGAQC
                     MIWDTSPAAAELEEKMMIWLRDLTGLPDDFEGVIQDSASTATLAAIITAREKSSGFLV
                     NEQGFPGSPVLRVYCSGQAHSSIEKAVKICGIGKANLVKIPVRKDFSMDPVLLEKAVS
                     DDLRKGYRPCCVVATIGTTGTTAVDPLREIGEICSRNGIWLHVDAAMAGTALILPEYR
                     WMTDGREYIDSFLFNPHKWMFTNFDCTAFFIKDAGRLIRTFEILPEYLRTRTRGQVND
                     YRDWGIPLGRRFRALKLWYVMRSYGAEGLREKIRNHIEYAANLKNLILAEDDFELLAP
                     VVINVVCFRYKPAGYSEEKLNALNEQLNHALNDSGKIYLTHTILNGIYTLRMVTAQTD
                     LTSAHVEKAWELIKTTARAITA"
     gene            complement(7026..7271)
                     /gene="atpC"
                     /locus_tag="PLZ75_07050"
     CDS             complement(7026..7271)
                     /gene="atpC"
                     /locus_tag="PLZ75_07050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOS72188.1"
                     /translation="MKVEIITPDHRVFEGEIKSIRVPGKKGSFQVLKDHAPIISTLEN
                     GPVIIVDMEGRETVFEINGGVIEVKMNKIIVLAESVN"
     gene            complement(7283..>8525)
                     /gene="atpD"
                     /locus_tag="PLZ75_07055"
     CDS             complement(7283..>8525)
                     /gene="atpD"
                     /locus_tag="PLZ75_07055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOS72189.1"
                     /translation="KGRLMNVTGDPIDGIGPLDKTGGYPIHRKPPKYDDLSTEKEVLY
                     TGIKVIDLIEPYSKGGKIGLFGGAGVGKTVVILELINNIAKAYSGLSVFAGVGERTRE
                     GNDLLREMIEAGVVSYGDKFLENMKAGGWDLSLIDPESLKESKLALVFGQMNEPPGAR
                     ARVALSGLSVAEHFRDGDGKSGGRDILFFMDNVFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGIMQERITSTKHGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRKISE
                     LGIYPAVDPLDSTSRILTPEIVGEAHYNCAQKVKELLQRYNELQDIIAILGMDELSDE
                     DKLVVHRARRVQRFLSQPFHVAEQFTGTKGAFVSIEDTIKGFNMIMDGEVDKYPEPAF
                     MLVGTIEDAIEKGEKILAQSK"
BASE COUNT         2200 a         2133 c         1987 g         2205 t
ORIGIN      
        1 ggcagaatcc tccgggctta cagttcctga ttcaattact ttccgggcat caacgggtat
       61 ccgtattgtc ctggagggaa tgatgtccag gagttccgtt gccgataccc tgacttttgt
      121 tgcgggatta tcacttttta tccagtcgat aatggtgcct atatccactg cttcctttgt
      181 tttctcaaca atgaaaacct ggttgttaat gccatcatag tattttttct ctgaaaacgt
      241 gaccggaagc ggatccgatt cataggtctt ccttttcatc tgatcaatat accagtcggt
      301 gttcagcagc atgaggttgc aaaccctcac atcggtacgc cttccctcaa cttcctgggc
      361 ataccacagg gggaaagtgt cattgtcgcc gtttgtgaag agaatggcat tgggcgcaca
      421 tgattcgaga taattgaaag caacgtcacg tgccagataa cggcccgatc tgtcgtgatc
      481 atcccagttc tcaaccgcca tgagggccgg aacggcggca aggcacagaa taccggccag
      541 aggggctgca acttttttgc ctgccagacg tgaaaaggct tcaaaaagcg caagtacccc
      601 aaaccctatc catacggcat agaaataaaa cgagccgaca taggcataat cacgttccct
      661 tggctggttt gggtactggt tgaggtaaaa gacaatcgct atcccggtca tgatgaaaag
      721 cagcatcacg atccaccagt tttttatatc acggttaaga tggtagaata atcctatcag
      781 gcccaggaaa aagggaagga gataatattt gttcctggat gtgtcattcc tttgttcagg
      841 gggcagatca cttgttccga cccttggctc atccaggaat ttaaagccgg agatccagtt
      901 gccgttgaca gctccgccgg ttccctgggt gtcattctgc ttcccggcaa aattccacat
      961 aaaataacgg aaatacatat aaccgtactg gtatgtaaac atgaatctca gattttcact
     1021 gaacctgggt ttccttatta ttgatttttc tcccgactgg ccggtcacct gaagcggaat
     1081 acccttcgtt ccgccccatt gcctgtacat ttcctcatga tccggctgat cactccacat
     1141 cctcggaaaa agcgtataaa atctttcatc aaaaaccctt tccagttcat gatgtgtgat
     1201 gatgtatttt ccgtcctcaa gggcataggc cggctttccg gtcttgtaat caagtaccgg
     1261 ggcattgtag taggctcctc ttatcagggg ccggctgccg tactgttccc tgttgaaata
     1321 gtacaggagg ttaaagggat tggacggatt attttcattg agcggcggat tggcagatga
     1381 cctgatcaca attattgccg aagaggaata tcctatcaga acgactgcca gggccgtgat
     1441 cacggtattg agggtgaccc tgctgccgga ggctatatac cagatgattc ctgccagtac
     1501 aaggaaaaca accagattga gcaggactga tccggaaatc acccatgctc ccgtaagaaa
     1561 aacagctgct gccgcaataa tgctgttctt aaccctgtct gccgaattga gggaataata
     1621 gatagccgct gtaaacaata caacaagaag gatgccgtga aaaatcattc cgctgttcac
     1681 aggaaggttc agcttgttta caaagaacaa atccagcctg gatgatattc tgactatacc
     1741 gggaataatt ccccacataa gaatggcaag gattataaac gagatcccga gagaaagcac
     1801 cagtcccttc caggtaaatt ccgatttttt gaaataatag acaaggacaa tggccggaag
     1861 cgccagaagg ttaagaagat gaacacctat tgaaagaccc atgagatatg ccaccaggat
     1921 tatccagcgg ccggcatgtt tctcttccgc ctcatcctcc catttcagta ttgcccagaa
     1981 ggatgcggcc gtaaaaaagg atgatgtggc atatacctcc ccttctacag cggaaaacca
     2041 gaatgaatcc gaaaaggtaa atgcaaggct tcccacgaca cctgcagcca tgatggcaat
     2101 aatatcagac agtgaagctt catttccgtt ccttaccatg attctcctgg ccaggtgggt
     2161 gatggtccag aataaaaata aaattgtgaa agcacttgca agagctgaca ttgcattaac
     2221 cattacagca acttttgcca catttccccc cgcaaaaagc gtgaagaacc tcgcaagaac
     2281 cataaaaacc tgactgcccg gaggatggcc tatctccatc ttataggctg atgcaataaa
     2341 ctccccgcag tcccagaagc ttgttgtcgg ctccattgtg agaagataag cagcagcagc
     2401 tattgcaaat accacccagc ccgtagtgtc gtttatcagt ttatattttt tcatcggccc
     2461 tgtatttgat atatcctgtc tttgctccaa cggcacgaaa gtactgaatt ttttgtttat
     2521 caccggcatt catgcacggt ccgcttaatt tgctattttt gagaattgtt tgatctgatg
     2581 aaaattaaaa ggcatatagt atctgcttta atctttcaga tttcattgct gctgtcagcc
     2641 caatactata ataccggtca ggatccggca ggactgaaat ggctgcagat aaagaccggc
     2701 cctttcagaa tcattttccc ggaggagtat gatgcggcag gaaaggaatt tgcaagggtt
     2761 cttgaggatg cacacaaggc agtgtcatat ccgggttttg caggcagata ccgcctccct
     2821 gtaataatcc acaattatac cacacagtca aacggatacg ttgcctgggc tcccaggaga
     2881 atggagattt accctacccc cgagcagaat tcgatacccc ttgacccctt tagccagctt
     2941 gcatatcatg agctgaccca tgtttaccag atggatacct tcagcagggg attttcgcgg
     3001 gcaatgatta tgtttttcgg acagcagttc ccgggagtgg tggcttcact tgtccctcaa
     3061 tggttcctgg aaggggatgc tgttttcaat gaatccttct tcaccccgtc gggcaggggc
     3121 agaacagcat cattccagaa atatctgaaa gccgtcagcc ttgaaagagg aaaaatgtat
     3181 aagtacgatc agcttgtaaa cggctcctac aggtatttta tacctgatca ttacgaatcg
     3241 ggttaccaga tcattgcatg gtcgaaactg agatatgaca gggatgtatg ggaaagggcc
     3301 ctcaggctga ccgccaatgc accttttctc gccaatccgg tgaacctgag cctgcggcac
     3361 agtatcggaa agacaaaaaa aatgctggct gatgaaacat ttgatactct gactgttata
     3421 tggaaagatg aaatagagct tcataatccc gcggaatatg aaatgataag tccccccgga
     3481 ggaaataaat atatcagtta tcacagcccg gtcaggatat cagagaacag atatgcagct
     3541 gtcagaactt cgctggacga gccccctgcc atcgtcatcg tcaacagcac ggacaggacg
     3601 gagcgaaaga tacagtcgcc gggaagcatg tacccctatg tgatctcatc cggcagggga
     3661 tttcttgcat gggtcgaaac ccggcctgat ccgagatggg caaacaggaa ctattccgtt
     3721 atcaggctta tggacacagg aaacggagtc acacggcagc tttcaaggaa gacgaggtat
     3781 atgtcggcag caatttcccc tgattcggaa ctaattgctg caagcgagaa taccgccgga
     3841 aataaaaaca acctggtgct gatagaccgc agaagcggga aaataatcaa atcagtaccg
     3901 gttcccggca atgccagtct gcagaagccc aggtggtcgg acgacggcag gaaaataagc
     3961 atgatcagcc tgactgccga gggagaagga atcatttcct tcagtttgca ggacatgaaa
     4021 tgggaaaagc tgatccccga aagccctgtg gattatcagt cggcagttat cagaaacgat
     4081 tccctcttct ttgtcagctc tgaatcgggt accgaaaatg tatacgtgct tacacctgat
     4141 aagagaacca taaggatcac cagtgtattc ttcggagcaa cagatcccct gcctgacggg
     4201 aaaaaaatcg ttttcagcaa ctattccctt acgggaaaca agctttgtat gaccgggatc
     4261 actgccgggg aggaagatgc cggttctgaa cgaactgccg gcatggaggc aggctatgaa
     4321 cgaactgccg gacaggatac tgtttataaa tccggttcag gaacggcttt cctgattgac
     4381 aggatcaaga cccccggaga acggacggaa actcccggag aacggacgga aactcctgaa
     4441 aaagagcggg aggaaaccgg gaaacagccg gaaacaagag aatatgtaac ggaaagatat
     4501 aataagtggc ggcatctttt taacattcac agctggatgc ctttttatgc ggatattgaa
     4561 gaaataaagg cagatccgct ggctgtcaga ccgggattca cggttttcag ccagaatcac
     4621 ctgagcactc ttacagcttc ggcaggatac gaatatagtg acaggctgca taaattacat
     4681 tccggaataa ggtgggaagg ctggtatccg gtatacgacg caggaataca ttacggtgac
     4741 aatccttcgg tactgaagtc aaaatcagaa gatcccgatc ccctatccgt aaacccagga
     4801 atacatttca ccaattcact ttcccttccc ctgcgttttt ccagcggcaa atactatcag
     4861 ttcctgcagc catccctttc tttgtcgttc cggaacaact acatatacgt taaggaagat
     4921 gcactgtatg actacggaca gaccatgact gccgccaggc tgtactttta caatgcccgc
     4981 agatcctctg tgcgtgacat ttatcccata tatgcccagg ttattgatct ttattattcc
     5041 tcatacccgt gggacaggga tttttatgcg ccgctggcca gtgtgcagac tgcattcttc
     5101 tttcccggac tgtttcccaa taattccctt cgcatcagac tggaaggcga gcagcaggga
     5161 tctgctgaat ttttgagaac caacaggtta cattttccaa ggggttaccg gaacatcata
     5221 tccgaaaaga tcttcttcgc ttcggctgat tacagggctc cgcttgcctg tcccgacctc
     5281 agcatcggca gcctcctgta tctgaaacgc ctgcgtgcag gcatattttt cgactatgcc
     5341 aggggcacca acaattatta cctgcaacac cgctcatcgg gcggattcag agtggaccat
     5401 gttcacagat atgctgaatc attcacttcc acgggagctg agctgatagc ggattttcat
     5461 gtcctgaggc ttccgtacat gatatctgca ggagtacagg ccgcgtggtc agcagacttc
     5521 gcatcgcccg tgcttgaggc aatattcagc attgatatcc atggaatgag tataggaaga
     5581 tcagggcccg gcagcatcag gctgtgatgg ccctcgccgt ggtttttata agttcccagg
     5641 ccttttcaac atgggcggat gtcaggtctg tctgagccgt aaccatccgc aatgtataaa
     5701 tgccgtttaa aattgtatgg gtaaggtaga ttttccctga atcattcagg gcatgattga
     5761 gctgttcatt aagcgcattc agtttttctt cactgtatcc cgcgggttta tatctgaaac
     5821 agacaacatt gatgactacc ggtgccagca gttcaaaatc atcctccgca aggatcagat
     5881 tcttcagatt cgccgcatat tcaatatgat tccggatttt ttcccgcagc ccttccgcgc
     5941 cgtatgatct catcacgtac cagagtttca gtgccctgaa cctccttccc aggggtatgc
     6001 cccagtcgcg gtagtcattc acctgtcccc tggtacgggt ccggagatat tcgggaagga
     6061 tctcaaatgt tctgatcagc cggcctgcgt cctttataaa aaaagccgtg cagtcaaaat
     6121 tcgtaaacat ccatttatga ggattgaaca ggaagctgtc gatatattcc ctgccatctg
     6181 tcatccaccg gtattcagga agtataagtg ccgttccggc catggcggca tcaacatgga
     6241 gccagatacc gtttctgctg cagatctccc ctatctccct cagagggtca actgccgttg
     6301 ttccggtggt tccgattgtg gctaccacgc agcatggcct gtaacccttc cggagatcgt
     6361 ctgatacagc tttttcaagc aatacgggat ccattgagaa atctttcctg acagggattt
     6421 taacaagatt ggccttacct attccgcata tttttactgc cttctcaatt gaggaatggg
     6481 cctgtccgga gcagtatacc ctcagtaccg gtgaacccgg gaatccctgc tcattgacca
     6541 gaaaaccgga tgatttttcc ctcgctgtaa taattgctgc cagggttgcc gttgatgcac
     6601 tgtcctgaat cactccctcg aaatcatccg gcaggccggt aaggtcccgg agccagatca
     6661 tcattttttc ctccagttca gccgcggcag gtgaggtatc ccagatcatg cactgcgctc
     6721 ccagggttgc ggtgagcatc tctgcaagga cggagggagg actgctgttg gcggggaagt
     6781 atgcaaaaaa attggggctc tgccagtgag tcatccccgg cattattatt tcatcgaaat
     6841 ccttctgtat cttttcaaag ggctcaggtt cctgtggggc cccgtccggc agcctgctga
     6901 atatctctcc gggttttacc cgcggtttaa cagggtattt ctccttgttt tcgagatagt
     6961 ctgttatcca ttctacaagc tcgtgcgcat gtttccggaa ttcctcggct gtcatgtccc
     7021 gtttttcagt ttaccgattc ggcaaggact attattttgt tcatttttac ctctatcacc
     7081 ccgccgttga tttcaaaaac tgtttcccgc ccttccatgt caacaataat tacagggccg
     7141 ttttccaggg ttgagataat cggggcgtga tccttcagaa cctggaagga tcccttcttt
     7201 ccgggtactc ttattgattt gatttcacct tcaaacaccc tgtggtcagg agtgataatt
     7261 tctactttca tcctgatgtc agttatttgg attgcgcaag aattttttct cccttttcta
     7321 tagcatcctc tatcgtgcca accagcatga atgccggttc tggatacttg tccacctccc
     7381 cgtccatgat catattgaat cccttgatcg tatcctctat ggaaacgaat gctccctttg
     7441 ttccggtgaa ctgctccgca acatggaagg gctgtgagag gaatctctgg accctccttg
     7501 ccctgtgaac cacaagcttg tcttcatctg aaagctcatc catgccgagg attgcaataa
     7561 tatcctgcag ttcattatat ctctgaagaa gttccttgac cttttgtgca caattgtaat
     7621 gggcttcccc tacaatttca ggtgtcagaa tacgcgatgt tgaatccagc gggtcaacag
     7681 cgggatagat accaagttcg gaaattttcc ggctgagcac cgtggtggca tcaaggtgtg
     7741 cgaaggtagt tgccggtgcg gggtcggtaa ggtcgtcagc cggcacataa acggcctgaa
     7801 cggatgtaat ggaaccatgt ttggttgaag tgatcctttc ctgcatgata cccatttcag
     7861 ttgccagcgt gggctgatat cccactgcag acggcattct gccaagaagg gcggatactt
     7921 ccgatccggc ctgggtaaaa cggaacacgt tatccatgaa aaacagaata tccctgccac
     7981 cgctcttgcc gtcaccatcc ctgaaatgtt ctgcaacgga caatcccgag agggccactc
     8041 ttgccctggc tcccggaggt tcattcatct gcccgaaaac aagtgcaagc ttcgattcct
     8101 tcagtgattc aggatcaata agcgagagat cccagcctcc ggccttcatg ttttcaagaa
     8161 atttgtcccc gtaggaaaca acgccagcct ctatcatctc gcggagcagg tcatttcctt
     8221 ccctggttct ttctccaacg ccggcgaaaa ccgaaagccc ggaataagct tttgcaatat
     8281 tgttgatcag ctcaagtatc acgacagttt ttcccactcc tgcgccgcca aaaagaccaa
     8341 tcttgcctcc cttggaatag ggttcaataa ggtcgataac ctttattccc gtgtaaagga
     8401 cctccttttc cgttgagaga tcatcatact taggcggctt gcggtggatc ggatagcctc
     8461 ccgtcttgtc aagaggtccg attccgtcaa taggatcgcc tgtaacattc atgagcctgc
     8521 ccttt
//
LOCUS       DAOSPX010000115         8525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7889_length_8525_cov_3.648642, whole genome shotgun sequence.
ACCESSION   DAOSPX010000115 DAOSPX010000000
VERSION     DAOSPX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_8525_cov_3.648642"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..940)
                     /locus_tag="PLZ75_07060"
     CDS             complement(29..940)
                     /locus_tag="PLZ75_07060"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOS72190.1"
                     /translation="MVKLDFNINDLHFKNPVLAASGTFGYGVEFRDFFDISQLGGIVL
                     KATTLEVREGNPYPRMAETPAGMLNSVGLQNKGIDHFEKNIYPVVSEFNTNVIVNVNG
                     SYVEDYVTLAGRINALEKIPAIELNISCPNVKKGGMIFGTDPKSAREVIREVRKVYSK
                     QLIVKLSPNVTDIVEFALIAEEEGADSVSLINTLLGMAVDVKRMKPSLATVTGGLSGP
                     AVKPVALRMVWQVAKAVRIPVIGLGGIMNSRDAIEFFLAGASAIQVGTASFINPAVTI
                     DILKGIENYLNEKGFSDIREIIGYINN"
     gene            complement(934..1701)
                     /locus_tag="PLZ75_07065"
     CDS             complement(934..1701)
                     /locus_tag="PLZ75_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOS72191.1"
                     /translation="MTKRIESLRVVENKKINKDYFLLRLTSQGKLPEMKPGQFVQVKV
                     DGSPETFLRRPFSVHDVNYSGNTFRLLIQIAGKGTETLSRLSTGDSINVIYPLGNSFS
                     LPEKNENILLAGGGCGIAPLLFLARYLNENGFTPDILLGFRSSERVMEYDEYAALGRI
                     FLTTEDGSMGKKGFITDHPVLTANRYDVVYCCGPEPMMKAMAAYCRANSVRCEVSLEN
                     LMACGIGVCLCCTVETVRGNLCTCTEGPVFNINDLKW"
     gene            complement(1728..2144)
                     /locus_tag="PLZ75_07070"
     CDS             complement(1728..2144)
                     /locus_tag="PLZ75_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOS72192.1"
                     /translation="MKERILEFLKAENKTSAQFAEEIGVQPSGISHILSGRNKPSLDF
                     VLKMLEKYTYISPEWLLFGREQMYRKQSAPDLFSQLQEEIFPSDSGITDVKEPVKEII
                     SEETPEREKETSSPAVEKIVWFYTNSSFREYYPEKE"
     gene            2979..4439
                     /gene="cls"
                     /locus_tag="PLZ75_07075"
     CDS             2979..4439
                     /gene="cls"
                     /locus_tag="PLZ75_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOS72193.1"
                     /translation="MEAFFNFINNSVILAVLYALVWIGVFLTILMENRNPAKSLAYIL
                     VLVFVPVLGLIIYYLFGRDFRKQKIFQKKGFGEEDISREYLSKYSRNSLEDLQFMEEK
                     IGDLVQPFRQLFLEKQSLIHSGNKVTLLTNGEEKFPALFRALEMAQHHIHIEYYIFSK
                     DDIGLKLTEILMQKASEGVRVRVIADDRGSRRIRDIPEMLRKAGVEFYSFMPVRFASL
                     SQANYRNHRKIVVIDGKSGFVGGLNLDDRYLNNGKHRLFWRDTHIKIEGPAVQELQLN
                     FLKNLSFVSKKHFELKAEYFPEASNYEDGAVVSVAASGPASPHPYNMEILLSAINRAR
                     KSIKITNPYFIPSDQLLLALELAVANGVLVELIIPARSDSFIVQHASFSFISPLIKRG
                     AKVYLYKKGFVHAKTMVVDGEICFVGTVNMDIRSFYINFEIAAVLHQQQLCEQMETQF
                     QKDKADSRLIIAADWKKRPKRKRFLDSLCRLLAPLL"
     gene            complement(4503..5087)
                     /locus_tag="PLZ75_07080"
     CDS             complement(4503..5087)
                     /locus_tag="PLZ75_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72194.1"
                     /translation="MKRIRRIAFAGLLTLFIFTLAGRNLYSQGIRNEKPPLRERIFFG
                     GDLGLQFGTITNIEIAPLVGVWLLPRVAVAAGPEYRYYAQKHFGGTNIFGGRAYTQLV
                     LIQDINNIIPAGLHLGLFLHAEDELLNLESKTPFWVNNQVTTDRFNINTFLVGGGVSQ
                     PMGRRSALNIIALWALNDPYGLYRSPDIRVSFTF"
     gene            5181..5873
                     /locus_tag="PLZ75_07085"
     CDS             5181..5873
                     /locus_tag="PLZ75_07085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS72195.1"
                     /translation="MNIIVNGATRGIGRETALLLAEKGNSVIITGRNEKALAELASLP
                     VAGKLIPCRLDISDFGRYEKMFMEIISSVFESVDILINCAAYLVVRDFSSFPPEEAVK
                     TMETNFFGVASMIRSIIPSMKKGSHIINISSMGGFQGSVKYRGLSYYSASKAALSCLS
                     ECLAAELLEEGIAVNCLALGSVQTEMFEEAFPGLRAPVRAGEMGEFIADFAIRGGKLF
                     NGKVIPVAFSNP"
     gene            complement(5860..6933)
                     /gene="buk"
                     /locus_tag="PLZ75_07090"
     CDS             complement(5860..6933)
                     /gene="buk"
                     /locus_tag="PLZ75_07090"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOS72196.1"
                     /translation="MPMRLILVINPGSTSTKFALYADEKLIMERNLTHSSGEVTGYGQ
                     IRGQLDFRRELILKELEEQKVNMSDIRVIVGRGGLVRPIESGVYRVNEKMISDLEKGL
                     LGQHASNLGGLIANALAAVMPNAEAYIVDPVVVDELQPVARISGHPSISRISIFHALN
                     QKAVARMYAASLEKRYEDMNLIIAHMGGGVSVGAHRRGRVVDVNNALDGDGPFAPERS
                     GSLPSGQLADLCFSGKHSYAEIRAMITGRGGMVAYLGTNSFREVCRKAEEGDAEAILV
                     RDAAAYQTAKEIGSAAAVLEGRVDAIILTGGMAFQEDHVERIKRMVGFIAPVIVYPGE
                     DELRSLAYNGLLALDGKISVRDY"
     gene            complement(7001..7912)
                     /locus_tag="PLZ75_07095"
     CDS             complement(7001..7912)
                     /locus_tag="PLZ75_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOS72197.1"
                     /translation="MVTSLDQMVGKVLSMKRRHRIAVAWAQDPGTIASVSRAVKEGFA
                     EAVLIGQTGKISDLCISRGIDPGLFTLISSADETDSAVTAVRLVRSGQADILMKGLVA
                     SDIFLRAIMDRNTGIMQQNAVLSYVGAIETPMYHKLLFVTDPAVIPYPDLRQKIAMIR
                     YAAGMTRRFGIEKPKIALIGASEKASSHFTYSSDYAIIKKMAEGGEFGSCIIDGPLDL
                     FLACDAKSTLIKGVDTPVAGDADILLFPSLEACNPFYKSLMLFGKGSLGGMIMGTDRP
                     VILMSRSESEQSKFYCIALACLMNGCK"
     gene            complement(7922..>8525)
                     /locus_tag="PLZ75_07100"
     CDS             complement(7922..>8525)
                     /locus_tag="PLZ75_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOS72198.1"
                     /translation="VGTSILLKEVLNKEWGLRTGKLLSHFALFEVETYHKVIAVTDVA
                     MNIAPNLQAKIAIINNSVGCLIKLGYIMPKVAVLGAVEMVNENMEATLDAALLSKMNQ
                     RDQIKNCIIDGPLAFDNAVSLESAKRKGIRSEVAGDTDLLLMPDIEVGNVLYKSLVFF
                     AKAKVASVILGAKVPIVLTSRSDSEQAKYDSILLSAAASK"
BASE COUNT         2302 a         1983 c         1783 g         2457 t
ORIGIN      
        1 atgattcagg aatataattt attgaatatt aattgtttat gtatccgatt atctctctga
       61 tatctgaaaa acctttttca ttcaggtaat tttcgatacc ttttaggata tcgattgtta
      121 ccgcggggtt aataaatgac gctgtaccca cctggattgc agaggcccct gccaggaaaa
      181 attcaattgc atcccgggaa ttcattattc ctcccagacc aattacggga atcctgacag
      241 ccttcgctac ctgccatacc atacgcaggg caaccggctt gacagccgga cctgacagtc
      301 ctccggtaac agtggcaagc gagggtttca ttcttttgac atccacagcc atgccaagaa
      361 gggtattgat aagtgaaacc gaatcagccc cttcttcttc agcaatcagt gcaaactcaa
      421 caatgtctgt tacattggga gaaagcttca ctatcagttg tttcgaataa accttcctga
      481 cttcccttat cacctcccgg gctgatttcg gatccgtgcc aaagatcata cctccctttt
      541 taacattcgg acaggagata ttaagttcta ttgccgggat tttctccaga gcattgattc
      601 tgccggccag ggtcacataa tcttcgacat agctgccgtt cacatttaca atgacatttg
      661 tattgaattc tgaaacaacc ggatagatgt tcttttcaaa atgatcaata cccttgttct
      721 gaaggcccac tgaattaagc atgccggcag gtgtttctgc catacgggga taggggtttc
      781 cctcgcggac ttccagtgtt gtggctttca gaacgattcc tccgagctgt gaaatatcaa
      841 aaaagtccct gaattcaact ccgtatccaa aagtaccgga tgcagccagt acaggatttt
      901 taaaatggag atcgtttata ttaaaatcaa gttttaccat ttaaggtcat tgatattgaa
      961 caccggtcct tccgtacatg tacaaagatt acccctgact gtttcaacgg tacaacagag
     1021 acatactcct ataccgcagg ccataaggtt ttccagtgag acttcgcatc tgaccgaatt
     1081 ggccctgcaa tatgctgcca tcgctttcat cataggctcg ggtccgcagc agtaaacgac
     1141 atcatatctg tttgcagtga gcacagggtg atcggtgatg aagccctttt tccccattga
     1201 gccatcctcg gttgtaagaa atatccgccc cagagcggca tattcatcat attccattac
     1261 tcgttcactg cttctgaaac caagcagaat atcgggagta aaaccatttt cattgagata
     1321 tctggccagg aacagcagag gtgctattcc gcagccacca cccgcaagaa ggatgttttc
     1381 attcttttcg ggcagggaaa aggaattgcc caggggataa ataacattaa ttgaatcgcc
     1441 tgtggacaat cgtgaaaggg tttcggttcc tttcccggca atctggataa gaagtctgaa
     1501 ggtatttcct gaataattga catcatgaac ggaaaacgga cgcctgagaa atgtttccgg
     1561 actgccatcg actttgacct ggacgaactg cccgggtttc atttccggca gttttccctg
     1621 ggaagtaagc ctgagcagaa aataatcctt gttaatcttt ttattctcaa caactctgag
     1681 agattcgatc cgttttgtca ttcaggcaga aatttcaggc aaatatacta ttctttttcc
     1741 gggtaatatt ccctgaaact gctattcgtg tagaaccaca caatcttttc aactgccggg
     1801 gatgaagttt ccttctccct ttcgggagtt tcttctgaaa taatctcctt taccggttcc
     1861 tttacatctg taattccact gtcagacgga aatatttctt cctgaagctg actgaacaga
     1921 tccggtgcag actgttttct gtacatctgc tcacggccga aaagcagcca ttccggagag
     1981 atataggtat atttctcaag cattttcagg acgaaatcca ggcttggttt gttccgtccc
     2041 gacagaatat gggaaatacc cgacggctgt acccctattt cttcagcaaa ctgtgccgaa
     2101 gtcttgttct cggcctttaa aaattcaagt attcgttctt tcatggatca ctattgtatt
     2161 acaaatgtaa acaattaaca attcacatgc ataactttta ttgatttaca gatgtaattt
     2221 tgaattatcg cgagaatgtt tttatcgata aatttctttt ttattaataa gattattccc
     2281 gaaattgcag atagaaatag caaatataat tgatgtctta ctggagatta gctgaatatt
     2341 ctacaggaac acagtttttt aagatgaatt tctaaagtaa tttgactgtt cagagcagga
     2401 aattaatgat tccatgtctg taaaagtata gaaaacaagt attaaattac tgataacaag
     2461 tcaaatatca aattaagtaa atataatacc atataattta cagatgtaaa ccagaattgt
     2521 taattaaatg gtttacgtgt gtgaacacaa taattgaatt ttgatgttta caaatgtgaa
     2581 ttaatgattt tgccatattt tctgatgcat tatccaaatg aattagtcaa catgtctttt
     2641 tcgacgatat ttcaggcctt aaatcttctg tctttaaaaa accgtttaaa aacaggttat
     2701 tccggagcca ttcaagaata tctctttatg ctgcagacaa aaggttccgc tgtcatgtat
     2761 cgtgcaaatt atatttcttt cattttaagc gcttcattca tgtggttttt gattaaaccc
     2821 agactgactg cccctctttt tgctatttta gcagggcagt cgtgattgtt aatgaaccgt
     2881 ctgcctgcct ggctttgaat aatcacctta atatatgtat tgcactcaca ttataaacaa
     2941 aggataaata tctccctgtc aatatcagaa cattatttat ggaagctttt ttcaacttta
     3001 tcaacaactc agtcattctg gcagttctgt atgctttggt ctggataggt gtattcctta
     3061 caattctgat ggagaacaga aatcctgcca aatcgctggc atatattctg gttttggttt
     3121 ttgttcctgt attgggcctg ataatttatt atctctttgg aagagatttc cgtaaacaga
     3181 aaattttcca aaagaagggc ttcggagaag aagatatctc cagggaatat ttgagtaagt
     3241 attcccgcaa ttccctggaa gaccttcagt ttatggaaga gaaaataggt gatctggtcc
     3301 agcctttccg ccagcttttc ctggaaaaac agtcattaat ccatagcggg aataaagtta
     3361 ctctgctgac aaacggagaa gagaaattcc ccgctctgtt cagggccctg gaaatggcac
     3421 agcaccatat tcacatagaa tattacatct tttccaaaga cgatattggt ctgaaactca
     3481 cagaaatcct gatgcaaaaa gcttccgaag gagtaagagt ccgggtaatt gcagatgaca
     3541 ggggaagcag gcgcatcagg gatattccag agatgctccg gaaagcagga gttgaattct
     3601 acagtttcat gccggtccgt tttgcttccc tgtctcaggc gaactaccgc aaccaccgta
     3661 aaattgttgt cattgacgga aagtctggtt ttgtcggagg ccttaacctg gacgaccgtt
     3721 atttaaataa tggaaaacac aggcttttct ggcgtgacac acatataaaa attgaaggtc
     3781 cggccgtaca ggaactgcaa ctgaattttt taaagaatct gagttttgtc agcaaaaaac
     3841 attttgaact gaaggctgaa tattttccgg aagcttcaaa ttatgaagat ggtgctgttg
     3901 tgagcgttgc tgccagcggt cctgcttcac cacaccctta caacatggaa atcctcctgt
     3961 cggcaataaa ccgggccagg aaatccataa aaatcaccaa tccctatttt attccttccg
     4021 accagctgct gctggctctg gaactggctg ttgcaaatgg tgtgctcgta gagcttatta
     4081 ttccagccag gtctgatagt tttatcgtgc agcacgcttc attttccttt atcagcccat
     4141 taataaaaag gggcgctaaa gtatacttgt ataaaaaagg gtttgtgcat gcaaaaacga
     4201 tggttgtgga cggtgaaatc tgctttgtgg gaacggtcaa catggacatc cgcagttttt
     4261 acatcaattt tgaaatagct gctgtactgc atcagcaaca gttatgtgag cagatggaaa
     4321 cgcagtttca gaaagacaag gccgacagcc ggctcataat agccgctgac tggaaaaaac
     4381 ggccgaaaag aaagcgtttc ctggattcgc tttgccgttt actggcaccc ttgttgtaaa
     4441 atgctgatat caggggttcc ggtatggctg agacacaaca aacctgcttt gcagaaacaa
     4501 gtctagaaag tgaaacttac ccggatgtca ggtgaacggt acagcccgta gggatcattc
     4561 agggcccaca gagcaataat gttcagggcc gatcttcttc ccataggctg gcttactcct
     4621 cctcccacaa gaaaagtgtt gatattgaat ctgtctgttg ttacctgatt attgacccag
     4681 aaaggtgttt ttgattccag gttcagcagt tcatcttcgg catgaagaaa cagcccgaga
     4741 tgcaggccgg cgggaatgat attgtttata tcctggatca gcaccagctg cgtgtatgcc
     4801 cgtccgccga atatattcgt gcctccgaaa tgtttctggg catagtatct gtattcggga
     4861 cccgcggcaa ctgcaacacg cggcagaagc cacactccga caagaggagc aatttcaatg
     4921 tttgttatgg ttccaaactg aagtccgaga tcccccccga aaaaaatcct ttccctcagc
     4981 ggaggttttt cattcctgat tccctgggaa tacagattcc ttcctgccaa tgtaaaaatg
     5041 aataaagtaa gtaatcctgc aaaggcaatc ctccggatcc gtttcaaata aaatacattt
     5101 atcatcatta cattttgtaa atttgacgtg atcttacaaa taaacgaaaa aatcactttt
     5161 attatatcag ccgtatcaaa atgaatatta ttgttaacgg ggcaaccagg ggaataggcc
     5221 gggaaactgc tcttctgctg gcagaaaaag gcaattcagt cataataacc ggaagaaatg
     5281 aaaaggcact ggcagaactt gcatcgcttc cggtggcggg aaagctgatc ccctgccggc
     5341 ttgatatatc cgacttcgga aggtatgaaa agatgttcat ggaaatcatc tcctccgttt
     5401 ttgaatcggt tgacatcctg ataaactgtg ctgcctatct tgttgtcagg gatttttcct
     5461 ccttccctcc ggaagaggcc gtaaagacaa tggaaaccaa ctttttcggc gttgcatcca
     5521 tgatccgcag cattatcccc tctatgaaaa aaggctcaca cattatcaac atcagcagca
     5581 tgggcggatt ccagggaagt gtgaaataca ggggattatc atattacagc gcatcgaagg
     5641 ctgcattgtc ctgtctgtcc gaatgtcttg ccgcagaact gctggaagaa ggaatagcag
     5701 tcaactgcct tgccctcgga tcggttcaga ctgaaatgtt tgaagaagct tttcccggtt
     5761 taagggcacc ggtcagggcc ggggaaatgg gggagttcat agctgatttt gccataaggg
     5821 gtggaaagtt gtttaacgga aaggtaatcc ctgtggcttt cagtaatccc tgaccgatat
     5881 ttttccatcc agtgcaagca gaccgttata agcaagcgac ctcaattcgt cttctcccgg
     5941 atatacaatt accggggcta taaatcccac cattcttttt atcctttcca catgatcttc
     6001 ctggaaagcc attcctccgg taagaattat tgcatccacc cttccttcaa gcacggctgc
     6061 ggccgatcct atttccttgg ctgtctgata ggctgccgca tccctcacca ggattgcttc
     6121 cgcatctccc tcttcagcct ttctgcatac ttccctgaaa ctgttggtgc caaggtaggc
     6181 caccattccg cctcttccgg tgatcattgc cctgatttcc gcatagctgt gctttccgct
     6241 gaagcagaga tcagccagct ggcctgaggg aagactgccc gatctttcag gcgcaaatgg
     6301 gccatcaccg tcaagtgcat tgttgacatc aacaaccctg cccctgcgat gtgcgccaac
     6361 actgactcct ccccccatat gggcaatgat gaggttcata tcctcgtatc ttttttccag
     6421 ggatgccgca tacatccgtg caaccgcttt ctgattgagg gcatggaaaa tcgaaatcct
     6481 ggatatcgat ggatgtcctg aaatccttgc caccggctga agctcatcaa ctacgacagg
     6541 atcaacaata taggcctcag catttggcat gaccgccgct aaagcatttg caatgaggcc
     6601 tcctagatta cttgcatgct gaccaagcag gcctttttca agatccgaaa tcatcttttc
     6661 gttcaccctg tacacccctg attctatagg ccttacaagt ccgcccctgc cgacaatgac
     6721 tctgatgtca gacatgttca ccttttgctc ttcaagttct ttcagaataa gttcccttcg
     6781 aaaatcaagc tgcccccgaa tctgcccgta tccggtcact tctccggatg aatgtgtcag
     6841 attcctttcc atgatgagct tttcatcagc gtaaagagcg aatttcgttg aggtggaacc
     6901 cggattgata acaaggatca gtctcatagg cattttaatt tttttcagtt cttcagacag
     6961 gacggatcat ggcattcaaa attaaaacat tatgctattt tcatttgcaa ccattcatca
     7021 ggcaggccag tgctatgcaa taaaatttcg attgttcact ttcgcttctc gacatgagga
     7081 ttacgggtct gtcagttccc atgatcatac ccccgagact gccctttccg aaaagcatca
     7141 gtgatttata aaagggattg catgcttcca gtgacgggaa caacagaatg tcggcatctc
     7201 ctgctaccgg ggtgtcaaca cccttgatca gggtactttt tgcatcacag gcaagaaaaa
     7261 gatccagggg cccgtcaatg atgcagctgc caaattcacc gccttctgcc atttttttaa
     7321 ttatggcata atcagagctg taggtaaaat ggctgcttgc cttttcagaa gcaccgataa
     7381 gggcaatttt cggtttttca atgccgaatc tccgtgtcat ccccgcagca taacggatca
     7441 ttgcaatctt ctgtctgagg tcgggatagg gtattactgc aggatcggta acaaatagca
     7501 gtttatggta catgggtgtt tcaattgccc cgacgtagct cagtacggca ttctgttgca
     7561 tgattcccgt attcctgtcc attatggctc tgaggaagat atcgctggcc acaagacctt
     7621 tcatcagtat gtcagcctgt cctgatctga ccagccgtac agcagttacc gccgaatcag
     7681 tttcatccgc agaactgatc agtgtaaata gtccggggtc aattcccctg cttatgcaca
     7741 gatcgcttat ttttccggtc tgtcctatta aaacagcctc ggcaaaccct tctttcacag
     7801 ccctgctgac agaagcaatg gtgccgggat cctgtgccca ggccacggca atacggtgcc
     7861 tccgtttcat agaaagcact tttcctacca tctgatcaag ggatgtgacc attgcggatt
     7921 ttcacttgct tgctgctgcg gaaagcaaaa tgctgtcata ttttgcctgt tccgaatcag
     7981 accttgaagt aagtactatc ggaacctttg cgcccagtat tacagaggct accttggctt
     8041 ttgcgaagaa aaccagtgat ttataaagca cattgccgac ttcgatatcc ggcatcagca
     8101 acaggtcggt atctcctgcc acttcacttc tgataccctt gcgttttgcg ctctcaaggc
     8161 tcaccgcatt gtcaaaggcc agcggaccgt cgataataca gttctttatc tgatcgcgct
     8221 ggttcatttt ggatagcaat gcagcatcaa gggttgcttc catgttttca ttcaccatct
     8281 caacggcacc aagcactgcc accttgggca ttatatatcc gagtttgatc aggcagccga
     8341 ctgaattgtt gattattgct attttggcct gaagattcgg cgcgatattc atagcaacat
     8401 cagtcacggc aattacctta tggtatgttt caacctcgaa cagggcaaaa tgggaaagca
     8461 gttttcctgt cctcaatccc cattccttgt taagaacctc cttgagcagt attgatgtgc
     8521 ccacc
//
LOCUS       DAOSPX010000116         8484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7924_length_8484_cov_4.739945, whole genome shotgun sequence.
ACCESSION   DAOSPX010000116 DAOSPX010000000
VERSION     DAOSPX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7924_length_8484_cov_4.739945"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /gene="trpS"
                     /locus_tag="PLZ75_07105"
     CDS             <1..810
                     /gene="trpS"
                     /locus_tag="PLZ75_07105"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOS72199.1"
                     /translation="VLSEYLACGIDPEVATVYVQSDVPEVTELYLLLNMNAYVGELER
                     TVSFKEKIRKQPDNINAGLLTYPVLMAADIIIHRANKVPVGQDQAQHLEMTRRFARRF
                     NTMYGVEYFPEPDAYNFGRKLIKIPGLDGSGKMGKSEGNGIFLADTPGEIRKKVMRAV
                     TDTGPATPGEKPSVPVQNLFTIMNVVSDPSTVKYFREQHSLGVIRYGDMKKQLAEDII
                     KVTEPVRERINQISSDSAYLARVVAEGAEKARASASRTIREVREIIGYRPF"
     gene            1002..3725
                     /locus_tag="PLZ75_07110"
     CDS             1002..3725
                     /locus_tag="PLZ75_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72200.1"
                     /translation="MGKKSAIISVIVLVTGLAVLGYFLRQGRKSMLTDPWGAVAPDAL
                     VIIESLDLKSFINSLTTGQGLFGEIAGIEKFGSFNRKLKFLADQLNKPAYQNILDGSR
                     ALVSFHTADSGKFRPLLILAVRTETRYGQLKDALKSSGIRTGRDILVAGVKTLVLPYE
                     DGGRKDSILAANLSGLILFTPSEELMRNAVIQAGRDGDVRTMPGFQRVLQASGKNEDK
                     IFIVYSNLPPLLKPAFRPGREETAGKAGRIAGSSGCDIHISNSGIIFSGYTESTDPAD
                     LLHRYKSGSAKAFSNYRVLPSSTVLFESVLYDAAKEVSGHYDGGNYTGQNTGRQSVPV
                     QAAELAEALREYTGDEITRALIDTRSKPAALNQVIIYELKNRAFAEKVFLDRFSSSIR
                     AGDIIFFRPDDQISIPVYLTPFKGYVSAFLENYSGEFSDSCFAFYDNYLVTGNSYSTV
                     SRFLYDNLLNKTLANDLVYRNFESSLPSMSEYLFYCVPSRISGYLSDFLSDEIMGIIR
                     ENRISLGRIPAAGFQLTPSNEMIYNCLSVQYREDVREESATEWETLLDTVAAIKPFFF
                     RNHITGAAEIFVQDQRNNAYLINAAGRILWKVPLQERINGGVNMIDYYRNGKFQLLFA
                     GRTHLHLLDRNGNYVERYPVRLRSPATNPPALFDYDNNRNYRIFIAGEDRMIYSYDKS
                     GSAVRGWKPFRTGSTVNAEISWFRVSGKDYLVVADENSVYFLDRTGNVRTRLREAVTK
                     ARGSAMRLLPGRTPAAVCTSPDGTIQLMHFDGRVEKFRVRDFSPEHSFDIFDIDGDGT
                     AEYIFIDDGVLYLYGHDRSEIFSRKFRSERLGGPISFTFSSNDRKIGIFEEDRELIWL
                     IGADGSAMEGFPLRGASMFSIGRLSDNTGWHLIVGGTDYFLYNYKIGSTSR"
     gene            3820..5292
                     /locus_tag="PLZ75_07115"
     CDS             3820..5292
                     /locus_tag="PLZ75_07115"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOS72201.1"
                     /translation="MKRYRLFSMIFIIALVFPRAQAQNSQTMYYMNLPQNHLLNPAIQ
                     PVSKVYIGLPAISGVNVDFSNNFFNFSDIFALNSTGESTISLDHPDFDAGNFIAGLRD
                     RNKIELRSAVQLFGLGISAGRDLYIFLDVNERIETNFGLPGDGLRLLFSGDDNFLGQS
                     VNMNSLAGDISWYRETGLGFSKNITSRLRIGVRGKWLSGVANISLENKGFSVTVKDDL
                     THQVDADLMANISAPVEILSDGDGMPDDISIDDSMFDDTRKMVDYFLKTGNNGVGLDV
                     GLEYLLSDRFRLSASVTDLGYIRWKRDLTNLQSKNQFVLSGVNFRDIYDGSIDFEELG
                     QELFDSLQKAFTVSATEIPYRTNIPAGLILGAAYSPSGSLTLGLLSHTRFDGNKFRET
                     LTFSANFNIGNAFSTSLAYTAAGYRYDNLGFGLAFRLGFFQIYALADRIPLKWNKFVS
                     DGKSIPVPEYLNTLHARAGMNLVFGNREMRKKDKPMILTQ"
     gene            5306..5485
                     /locus_tag="PLZ75_07120"
     CDS             5306..5485
                     /locus_tag="PLZ75_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72202.1"
                     /translation="MVLLLRVFLIGLIVYLLVRPFLAYRRGTYEQDRVDYNAGRDKKI
                     SKDTGEYVEYEEIKD"
     gene            complement(5489..6247)
                     /locus_tag="PLZ75_07125"
     CDS             complement(5489..6247)
                     /locus_tag="PLZ75_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS72203.1"
                     /translation="MIRIKGLEKAFNDKVVLHDINVSFESGKTNLIIGKSGSGKTVLL
                     KSIAGLHEPDKGEVWFGDILFNKLDFNSKKEIRKEMGMLFQGSALFDSLTVEQNVKFP
                     LDMFTLETPEEKIERVNFCLNRVGIVNANRLFPSELSGGMKKRVAIARAIALNPKFLF
                     CDEPNSGLDPVTAIMIDALIREITEEYSMTTIINTHDMNSVIEIGENIVFINDGYICW
                     EGNKDEVLRSDNELLNEFIFANSMAKHLLTPRKK"
     gene            complement(6244..6987)
                     /locus_tag="PLZ75_07130"
     CDS             complement(6244..6987)
                     /locus_tag="PLZ75_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS72204.1"
                     /translation="MLRFLSQAGKYFLLLKKVFSRPEKPTVYLRQTMFELHNLGFNSV
                     GIISIISFFMGAVIVLQTAYNTENPIYPTYLIGLTCRDMLLLEFSSTVSGLILAGKVG
                     SSIASEIGTMRVTEQIDALEMMGINSASYLILPKVIATLLFNPVLTLLSIVVGTLGGW
                     LAGTTAGVITSEDFIYGIQYAFIPYYVTYAIIKTLIFAFIITSISSFQGYYVEGGSLE
                     VGRASTKAVVFSSILILLFNVILTQTMLS"
     gene            7119..8204
                     /locus_tag="PLZ75_07135"
     CDS             7119..8204
                     /locus_tag="PLZ75_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOS72205.1"
                     /translation="MKDRYVIIMAGGVGTRFWPLSRQQRPKQFLDILGTGETLIQQTY
                     RRFKDCCPEKNIFIVTSDEHKDIVVEQLGIDPSQVLAEPLRRNTAPCLAYGTFRILKE
                     NPDAVVAVTPADHLILKEEEFTRVISESMDFARGNDVLLTLGIKPNRPETGYGYIQAD
                     LKKPVEGYENLLKVKTFTEKPKIDLARKFLQSGDFYWNSGIFIWNVKAILQAFEKHLP
                     DMYSAFSEGRDLFGTDAETGFIGSTYIECRSISIDYGIMEKADNVYVRCTEIGWSDLG
                     TWGSLYEHSANDMKSNTIISGNVFSYENAGNIISISDGKVAVIQGLKDYIVVETGDVL
                     LIVKKEEEQNIRNFLEDVRKETGEQFM"
     gene            complement(8289..>8484)
                     /locus_tag="PLZ75_07140"
     CDS             complement(8289..>8484)
                     /locus_tag="PLZ75_07140"
                     /inference="COORDINATES: protein motif:HMM:NF040058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72206.1"
                     /translation="NNDAQRLSFDDLFMQRRFGSIIFGESNVFNDRMVSEYLVGKAAL
                     FEADRLKQELFDLEHDLWEF"
BASE COUNT         2339 a         1812 c         2154 g         2179 t
ORIGIN      
        1 gtcctttcgg aatacctggc atgcggcatt gatccggaag ttgcaacggt atatgttcag
       61 agcgacgttc ccgaggtaac cgagctttat cttctgctta acatgaacgc ctatgtgggt
      121 gagcttgaaa ggaccgtgtc gttcaaggaa aagataagga agcagcctga caacattaat
      181 gccggacttc ttacctatcc cgtccttatg gccgcggata tcataatcca cagggcaaac
      241 aaggttcccg tgggacagga ccaggcccag catctggaaa tgacaagacg ctttgcaagg
      301 cgcttcaaca ccatgtacgg tgttgaatat tttcctgaac cggatgccta taacttcggc
      361 aggaaactga tcaagattcc cggacttgac ggcagcggca aaatggggaa gagtgagggt
      421 aacggcatat tcctggcaga tacgcccggg gaaatcagga aaaaagtcat gcgggcggtt
      481 accgacacag ggccggccac accgggagag aaaccttccg ttccggtaca gaaccttttt
      541 acaatcatga atgtggtttc ggatccctca actgtgaagt atttcaggga gcagcattcc
      601 cttggagtga tccgctacgg ggacatgaag aaacagctgg ccgaggatat cattaaagtc
      661 acagaacccg tgagggaacg cataaaccag atcagttccg acagtgccta tcttgccagg
      721 gttgtggccg aaggagcgga gaaagccagg gcgagtgcat cacggaccat acgtgaggta
      781 cgggagataa taggatacag gccattctga aaaaaagaaa gccaggtctt tgacctggct
      841 gcacttgatg gatcaagtat atgattgaaa ttgcctgtat cttctgcagt tcaaataacg
      901 tgccaaaatt ggtgagacaa aatggcagaa agtgattgat ctgtgctggc aataatttcc
      961 attatttgat taactttaac catggaattt aatattctgt tttggggaaa aagtctgcga
     1021 ttatctcggt tatagtgctt gttacagggc ttgcagtgct gggatatttt ctccggcagg
     1081 gcaggaaaag catgctgacc gatccctggg gggcggtggc cccggatgca ttagtcataa
     1141 ttgaaagcct ggaccttaaa agttttataa actcacttac aacaggccag ggcctgttcg
     1201 gcgaaattgc cgggatagaa aaattcggta gtttcaaccg gaaactcaaa ttcctggcgg
     1261 atcagcttaa taaacccgct tatcagaata tccttgacgg cagcagggcc cttgtttcat
     1321 ttcatactgc cgacagcggg aaattccgtc cgctcctgat tcttgccgtc agaacggaaa
     1381 cccggtacgg gcagctgaag gatgccctga aatcctcagg gatcagaacc ggcagggata
     1441 tacttgtggc aggtgtgaaa acgcttgtcc tcccctatga ggacggggga agaaaagaca
     1501 gtattcttgc tgctaacctt tccggactta ttctgttcac cccttcggag gaactgatga
     1561 ggaatgctgt cattcaggca ggccgtgacg gtgatgtgag aacaatgccc ggttttcaga
     1621 gagtactgca ggcttcagga aaaaatgaag acaagatttt cattgtctat tccaatcttc
     1681 ccccgcttct gaaaccggct ttcagacccg gccgggagga aacagccggt aaagccggaa
     1741 ggattgcagg aagttccgga tgcgacattc acataagcaa cagcggaatt atcttcagcg
     1801 gatatactga aagcacagac cccgctgacc tccttcacag atataaaagc ggttctgcaa
     1861 aggccttttc caattacagg gtgctgccgt catcaacagt actttttgaa tccgtgctgt
     1921 atgatgctgc aaaggaggta agcgggcact atgacggggg aaattatacg gggcagaata
     1981 ccggcaggca gtctgtaccg gttcaggcag cggagctggc ggaagctctg agggaatata
     2041 ccggtgatga aataaccagg gcattgatag atacaaggtc aaaacctgca gctttaaacc
     2101 aggtgataat ctatgaactg aaaaaccgtg ccttcgcgga aaaggtcttt ctggacaggt
     2161 tcagcagcag catcagggcc ggagatatta tctttttccg gccggacgac cagatcagta
     2221 ttccggtgta tctgaccccc ttcaagggat atgtctcggc cttccttgag aattattccg
     2281 gggaattcag cgattcatgc tttgccttct atgacaacta ccttgtaaca ggaaattcat
     2341 actccaccgt ctcgaggttc ctttatgaca acctgctgaa caagactctt gccaacgatc
     2401 tcgtttacag gaattttgaa agctcccttc caagtatgtc ggaatatctc ttttactgcg
     2461 tcccttccag gataagcgga tatctgtcag acttcctgtc ggatgagatc atgggtataa
     2521 tcagggaaaa caggatttcc ctgggcagga ttccggcagc aggttttcag cttactccga
     2581 gcaacgaaat gatttacaac tgcctgtcag ttcagtacag ggaggacgtc agggaggaat
     2641 ccgcaactga atgggaaaca ctgcttgaca cggttgcggc catcaagccc ttcttcttca
     2701 ggaatcatat aacgggagct gctgagatct ttgttcagga ccagaggaac aatgcttacc
     2761 tgatcaatgc tgccgggagg atactgtgga aggtcccgct tcaggagagg ataaacggcg
     2821 gggtgaacat gattgattat tacaggaacg gaaaattcca gctgcttttt gcaggcagaa
     2881 ctcatctgca tcttcttgac agaaacggca attatgtgga aaggtatcct gtcaggctca
     2941 gatcgccggc cacgaatcct ccggccctct tcgattacga caacaacagg aattacagga
     3001 ttttcattgc cggagaggac aggatgatct attcctatga caagtcgggc agtgccgtca
     3061 ggggatggaa gcctttcagg accggaagca cggtgaatgc ggagatttca tggttcaggg
     3121 tgtcaggaaa ggattacctg gttgttgccg atgaaaactc cgtttatttc ctcgaccgta
     3181 ccggaaatgt aagaacaagg ctcagggaag ccgtgacaaa ggcccgggga tcagccatga
     3241 ggcttttacc gggaagaact ccagcggcag tatgcacttc gcctgacggg acaatacagc
     3301 tcatgcattt tgacggcaga gtggagaaat tcagggtcag ggatttttcc ccggaacaca
     3361 gctttgacat tttcgatatt gacggtgatg gcacggcgga atatatattt atagatgatg
     3421 gtgtattgta cctgtacggc catgacagat cggagatttt ctccaggaaa ttcagatcgg
     3481 aaaggctggg aggacccatc agttttactt tttcttcaaa tgaccgcaaa atcggaatct
     3541 ttgaagagga cagggaactg atctggctta tcggggctga tggttcggca atggaaggat
     3601 ttcccctccg tggagcatca atgttctcga ttggcagatt atcggataac acaggctggc
     3661 atcttattgt cggcggaacg gactattttc tgtataatta caaaatagga agcacttcca
     3721 gatgatttta cgtataagga agaatgtgat gtcattgttg atccctgcag ctgttgcagg
     3781 aaagaaaaat atatgttcaa aaaaccaaat ctgacagata tgaagcggta cagattgttt
     3841 tccatgattt ttattatcgc cctggtattt ccccgggctc aggcacagaa cagccagaca
     3901 atgtattata tgaatctgcc tcagaaccat ttgcttaatc cggccattca gcctgtaagc
     3961 aaggtttaca taggcctgcc cgccatctcg ggagtcaatg tggatttcag caataacttc
     4021 tttaatttca gtgatatttt cgctctgaat tctacgggtg aatcaaccat ttccctcgat
     4081 catcccgatt ttgatgcagg aaatttcatt gccggactga gagaccggaa caagattgag
     4141 ctgcgctcgg cggttcagct gttcggactg ggtataagcg cagggcgtga cctgtatatc
     4201 ttcctggatg taaatgaaag gattgaaacg aatttcggac tgccgggtga tgggctcaga
     4261 ctgcttttca gcggggatga taactttttg ggacaatccg tcaatatgaa ttcccttgca
     4321 ggcgatatca gctggtacag ggaaaccggc ctgggcttct cgaaaaatat tacaagcagg
     4381 ctgaggatag gagtcagggg aaaatggctt tcgggagtgg caaatatatc acttgaaaac
     4441 aagggcttca gtgtaacggt taaagatgat ctgactcacc aggtggatgc cgacctgatg
     4501 gcaaatataa gtgcaccggt tgaaatcctt tctgacgggg atggtatgcc ggatgatata
     4561 tctatcgatg attccatgtt tgatgatacc cggaagatgg ttgattattt ccttaagacg
     4621 gggaataatg gtgtaggact ggacgtcggg cttgaatacc ttttgtctga caggttcagg
     4681 ttgtcggctt cagtgacaga cctggggtat atccgctgga aaagagacct cacaaatctg
     4741 cagtcaaaga atcagtttgt actcagcgga gttaacttcc gggatattta tgatggaagc
     4801 attgatttcg aggaactggg acaggagctt ttcgattccc tgcagaaagc ctttaccgtt
     4861 tcagccacgg agataccata caggaccaat ataccggccg ggttaatact cggtgccgca
     4921 tacagccctt caggcagtct taccctgggc cttctttcac atacaaggtt tgacgggaac
     4981 aagttccgtg aaactttaac attctcagcc aactttaata tcggaaatgc cttttccaca
     5041 agtcttgcct atacagcagc cggttacagg tatgataacc tcggattcgg cctggcattc
     5101 cggctgggat ttttccagat atatgcactt gctgaccgga ttccgctgaa atggaataaa
     5161 tttgtatccg atggcaaaag cattcccgtt ccggaatatc ttaatacatt gcatgcacgg
     5221 gcgggaatga atctcgtatt cggaaacagg gaaatgagaa agaaagacaa acccatgata
     5281 ttgacccaat aaaaattaag ttacaatggt acttttactg cgtgtttttt taataggtct
     5341 gattgtgtat ttactcgtca ggccctttct cgcgtacaga aggggaacat atgaacagga
     5401 cagggtggat tacaatgccg gaagggacaa gaaaatctca aaggatacgg gtgagtatgt
     5461 ggaatatgag gaaataaagg actgatcctt atttttttcg tggtgtaagc aggtgtttgg
     5521 ccattgaatt tgcaaaaata aactcattca gtaattcatt gtcggagcgg aggacttcat
     5581 ccttgttgcc ttcccagcat atgtatccgt cattgatgaa tacaatgttc tcgcctatct
     5641 cgatcaccga gttcatatca tgagtattta tgattgtggt catggaatat tcctcggtta
     5701 tctccctgat aagcgcatcg atcataatgg cagtgacagg atcaagtcct gaattgggtt
     5761 catcgcagaa caggaatttc ggattgaggg caatagccct ggctatggcc acccttttct
     5821 tcattccgcc cgatagctcc gacggaaaaa gcctgtttgc atttacaata ccaacccggt
     5881 tcaggcagaa attaaccctt tcaatctttt cctccggcgt ctcgagggta aacatatcga
     5941 gagggaattt cacattctgt tcaaccgtga gggaatcaaa gagggcgctt ccctgaaaaa
     6001 gcattcccat ttccttgcgg atctccttct ttgaattgaa gtcaagctta ttgaaaagga
     6061 tgtcgccgaa ccatacttcc cctttgtccg gctcatgcag gcctgcaatg gatttcagaa
     6121 gcactgtctt gcccgaaccg cttttgccta tgataaggtt tgttttgccg ctctcgaatg
     6181 acacgttgat atcatgaagt accaccttgt cgttgaatgc cttttccagg ccttttatcc
     6241 ttatcatgac agcatcgttt gagttaaaat gacattgaaa agcagtatca ggatgctgct
     6301 gaacacaacg gctttggtac tggcacggcc cacttcgagg gacccgccct caacataata
     6361 tccctgaaag gaagaaatac ttgttattat aaaggcaaag atgagtgttt tgatgatggc
     6421 ataagtaacg taatagggaa tgaatgcata ttgtatcccg taaatgaaat cttcggatgt
     6481 tattacgccg gccgtggttc ctgccagcca gcctccgagt gtcccgacca caatactcag
     6541 gagtgtgaga accggattga acagaagagt tgcaatgact ttgggaagta tcagatagga
     6601 tgccgaattg atccccatca tctcgagtgc gtcaatctgt tccgtcacac gcatggttcc
     6661 tatttccgag gcaatacttg atcccacctt cccggcaagg atcagtccgc ttaccgtgga
     6721 tgagaattca agcaggagca tgtcgcggca ggtgagtccg ataagatatg tcgggtaaat
     6781 tggattttca gtgttgtagg ctgtctgaag gacaattacg gctcccatga aaaaggaaat
     6841 gattgaaatt attccgacag aattaaatcc gagattatgc agctcaaaca tggtttgcct
     6901 gagataaaca gtaggctttt cgggccttga aaacactttc tttagcaaaa ggaaatattt
     6961 gcctgcctgt gaaagaaatc tgagcatgtc ccggtgtatt ttagacagct taaaaataca
     7021 aaattttgcc ggatttttgc catgacaggc attaatcagg ccggaaggat atttttagtt
     7081 atatatttgc agcaagttac cagatgaaaa gatcaatgat gaaagacagg tatgtgatta
     7141 ttatggccgg aggtgtcgga accagattct ggccgctgag caggcagcag aggccgaagc
     7201 agttcctcga tatcctggga acgggtgaaa ccctgataca gcagacatac aggaggttta
     7261 aagattgctg tccggaaaaa aacatattta tcgtaacaag cgatgaacat aaggatattg
     7321 ttgttgaaca acttggaatt gatccctcgc aggttctggc agagcccctg aggcggaata
     7381 ccgcaccctg cctggcttac ggtacattca ggatactgaa ggaaaatcct gacgctgtgg
     7441 ttgcggtgac acctgctgat catcttatac tgaaggagga ggaattcacc agggttatca
     7501 gcgaaagcat ggacttcgcc aggggaaatg atgtgctgct cactctggga ataaagccca
     7561 accggcctga aacaggttac ggatatatac aggccgatct gaaaaaaccc gttgaaggat
     7621 atgagaacct gctcaaggta aaaaccttca ccgagaaacc gaaaatcgac ctggcaagga
     7681 agttcctgca aagcggggat ttttactgga attccgggat ttttatctgg aatgtgaaag
     7741 caatactgca ggcctttgaa aagcatctgc ccgatatgta ttcggctttc agcgaaggca
     7801 gggacttgtt tggcacagat gcggaaaccg gtttcatcgg gagcacctat attgagtgca
     7861 gaagtatttc gattgactac gggataatgg aaaaggcgga taatgtgtat gtgaggtgta
     7921 cggagatcgg ctggtccgat ctgggtacat ggggttcatt gtatgagcat tcggcaaatg
     7981 atatgaaaag caacaccatc atcagcggaa acgttttctc atacgaaaat gccggtaaca
     8041 ttatcagtat ttccgacggg aaggtggcgg taattcaggg attgaaggat tatatcgtgg
     8101 ttgagaccgg tgatgttctg ctgatagtga aaaaggaaga agaacagaat atcaggaatt
     8161 ttctcgagga tgtgagaaag gaaacgggag agcagtttat gtgatcagaa ggttataaaa
     8221 aaagaggctg tctcctggtg agcagcctct tttttataat gcatatcatg gaatttccat
     8281 gattcgtact agaattccca caaatcgtgt tcaagatcga atagttcctg cttcaggcgg
     8341 tcggcttcga aaagggctgc cttcccgaca aggtattcgc ttaccattct gtcgttgaag
     8401 acattcgatt ctccgaatat tatgcttccg aatcttcttt gcatgaagag atcatcaaag
     8461 gacagcctct gggcatcgtt attg
//
LOCUS       DAOSPX010000117         8446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_7965_length_8446_cov_4.108450, whole genome shotgun sequence.
ACCESSION   DAOSPX010000117 DAOSPX010000000
VERSION     DAOSPX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7965_length_8446_cov_4.108450"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1221)
                     /locus_tag="PLZ75_07145"
     CDS             complement(184..1221)
                     /locus_tag="PLZ75_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOS72207.1"
                     /translation="MTDRKNETSVLIIYTGGTIGMVHDPETGSLIPIDFKHITDHVPV
                     IGNYGFELHSVSFDPVKDSSNIDPDIWIRLAGIIEENYDRFDGFVVLHGTDTMAYSAS
                     ALSFMLENLSKPVIFTGSQLPIGLPRTDGRENLITAIEIAAAKKNGNPIVPEVCIYFD
                     SELSRGNRTTKFSAEHFDAFNSPNYPLLAEAGLHITYNTGIIRYPEKIRKLKVHRKFD
                     NNVAILKLFPGLNRNFVKAVTGTAGLKGLIIETFGSGNAPTYKWFLDDLKAFIRKGGI
                     IFNVTQCHGGSVEMGLYETSREMLAAGVISGRDITSEASVTKLMLLLGCHGSAEKVAE
                     MLAKPIAGEMS"
     gene            complement(1214..1993)
                     /locus_tag="PLZ75_07150"
     CDS             complement(1214..1993)
                     /locus_tag="PLZ75_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOS72208.1"
                     /translation="MKLIDTHTHLYLPAFRNDRDEMIGRSAGRGVVRLLMPNIDIHSV
                     EPMLKAAEEYRGICLPMMGLHPTSVGDDYTDQLDRMEKILPQHAFIAVGEIGIDLYWD
                     KSRFAEQVAAFRRQLDLAVKSGLPVVVHSRNSIDEVLSVMEEYTASGLRGVLHAFSAG
                     LSYAEKAVSMGLFLGIGGPLTYKNSRLGDICREIGPRHIILETDSPYLAPVPYRGKRN
                     ESSYIPIINKKLAEVLGMSEEETASITFENSCRLFNLLPYD"
     gene            complement(2054..3166)
                     /gene="dprA"
                     /locus_tag="PLZ75_07155"
     CDS             complement(2054..3166)
                     /gene="dprA"
                     /locus_tag="PLZ75_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOS72209.1"
                     /translation="MPKNLSLKHKIALGIIPRIGDINARKLVSHLGSVEAVFSESYRN
                     LIRIPGIGSELAKCITERSYLEKAEQEALYVTRNNIRTYFYLDDDYPSRLRQCEDSPV
                     MFFLKGSCDPDAPRILSVVGTRNSTSRGREICEKIIGDLAADFPGLTIVSGLAYGIDI
                     TSHKAALANNLPTIAVLGHGLNTIYPSVHRPLAGKIAENGGLLTDFLSDTLPERNNFI
                     KRNRIIAGISDATLIIESGKKGGALITADIAGSYNRDVLAVPGRPGDIWSAGCNSLIK
                     YNRAALVESAGDVKYFLNWQPEKTGDPQQKKLFPELGETEKSIYDLLSAGSEMTIDSI
                     CRSLDLPVFKLSAILLQLELKGLIKNCPGNIYRIAL"
     gene            complement(3185..4522)
                     /gene="gdhA"
                     /locus_tag="PLZ75_07160"
     CDS             complement(3185..4522)
                     /gene="gdhA"
                     /locus_tag="PLZ75_07160"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOS72210.1"
                     /translation="MNVDKIMNDLEKKHPGEVEYLQAVREVLESIVDIYNENPQFRSA
                     NIIERLIEPDRVLIFKVPWTDDEGNVRVNLGYRIQFNCAIGPYKGGLRFHQSVNQSIL
                     KFLGFEQIFKNALTSLPLGAGKGGSDFSPKGKSNAEVMRFCQSFMLELWKYIGPETDV
                     PAGDIGVSGREIGYLYGMYKRLKGAHTGVLTGKGLNWGGSLIRPEATGFGAIYFVQEM
                     LAIRKETIEGKVISISGFGNVAWGVALKATQLGAKVVTISGPDGYIYDPQGVSGRKIE
                     YMLELRASNQDIVSPYAEMFDGVEFHPGKKPWEVKVDIALPCATQNELNAEDAKKLVG
                     NGVMMVAEVSNMGCTPEAIEVFYDNDVIFAPGKAVNAGGVATSGLEMAQNAMKMRWTR
                     QEVDQRLHEIMHNIHEQCLKYGTQPDGFIDYVKGANISGFLTVANSMLDMGIV"
     gene            complement(4625..5107)
                     /locus_tag="PLZ75_07165"
     CDS             complement(4625..5107)
                     /locus_tag="PLZ75_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72211.1"
                     /translation="MTFDNTKQIISIRLKLFFATVLILIYLALVYIGKIIRFPVLGLS
                     DSAWTIILVSCYLIIFFTPFILNYQFIYYSDDDENIVLRYFNAGMGGGKKNSIRIHKN
                     TFAGYKTESGLFGLKQSIILYQKISQGTAKYPPVHLSALNREQKNRLFSALDLLLPKS
                     "
     gene            complement(5122..5571)
                     /locus_tag="PLZ75_07170"
     CDS             complement(5122..5571)
                     /locus_tag="PLZ75_07170"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOS72212.1"
                     /translation="MKANTHEYFMKAALEEARKAFEKDEVPVGAVVVCNGIIIARAHN
                     LIETLRDPTAHAEMQAITAAAGWLGGKYLTDCTVYVSIEPCAMCAAALGWSHAGRLVY
                     GAPDEKKGYTLISDRLLHPKTKVTGGILEEECREIMLRFFKKKRLSL"
     gene            5693..7459
                     /gene="aspS"
                     /locus_tag="PLZ75_07175"
     CDS             5693..7459
                     /gene="aspS"
                     /locus_tag="PLZ75_07175"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOS72213.1"
                     /translation="MYRTHTCGELRTGDAGKEVTLCGWIQTSRDLGGMTFIDLRDRYG
                     ITQLVFNMETNPGLCEQARKLGREFVIQVTGNVRERSNKNLRIPTGEIEIEAARFVVL
                     NPSELPPFTIEEDTDGGDELRMKYRYLDLRRPSVRDNMILRHRMAREVRNYMDSAGFI
                     EIETPFLIKSTPEGARDFIVPSRLQPGSFYALPQSPQTLKQLLMVSGFDRYFQIVRCF
                     RDEDLRADRQPEFTQIDCEMSFVTRDDILDTFEGLVRHLFKSVKGIEFSEPFIRLTYD
                     EAMESYGTDKPDLRFGMEIRDLTGIVKGRGFSLFDNSGYIGAISVPGCSGFSRKQLDE
                     LTGFVTRQQSGAKGMVWLKYEADGTLRSSVDKFYSAEDLMLWVEKLQSGRGDLVLVLF
                     GDREKILPALSGLRLEMGNRLNLRKRDEYKPLWVVDFPLFEWNEEETRFYAMHHPFTS
                     PRPGDIQLMETDPGAVRADAYDLVINGIETGGGSVRIHDASLQKLMFKTLGFTEKEAA
                     EQFGFIMNAFRYGAPPHGGIAFGFDRLVALFGGQDSIRDFIAFPKNNSGRDVMLDTPA
                     PVSEKQLQELMLKPSGRKQGQE"
     gene            complement(7462..8199)
                     /locus_tag="PLZ75_07180"
     CDS             complement(7462..8199)
                     /locus_tag="PLZ75_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOS72214.1"
                     /translation="MKTLINRVTACRLILTALLSMFIIQGLAGQKKAKKFFVSGVVTD
                     ARGIPAEGAIIFVDNKSTDRVSDKNGFFRVKVKPDAGIISALHTMAGLGEAVIDGENV
                     LSIKLNGAVGFPLPETQTRSDEQIDIGYGTVDRKKLITPVTSVNDVSSKYASYNNIYD
                     VLKGTVPGVQVKDRKIIIGGPSSFNLSTEPLFIVDGMEVPSIDEIQPVMVESIEVLKG
                     PSASIYGVKGANGVILIRLIGTKKPGK"
BASE COUNT         2061 a         1998 c         1996 g         2391 t
ORIGIN      
        1 gccagttaaa ccactcctgc atctctccct gtgtctttca ggcgtatcag aaagtaagag
       61 ttttaatccc ggcagttttc acttcctgat caatgcttct gtttccgtga gtctttccgg
      121 acgggaaaca gtacattgcc ggctgaacgg cggcgcaaat tacaaaattt ttcaatacaa
      181 actttatgac atttcccctg caatgggttt tgccagcatc tctgccacct tttccgctga
      241 accgtggcag ccgagcagga gcatcagctt ggttacggat gcttcggatg ttatgtctct
      301 tccgcttata acacctgctg ccagcatctc cctgctggtt tcgtacaaac ccatttcaac
      361 actgccgcca tggcactgtg tgacattgaa tattatgccg ccctttcgga taaatgcttt
      421 gagatcatcc agaaaccatt tgtaggtagg agcatttccc gagccgaagg tctctattat
      481 aaggcctttc agacccgcag tgcctgtaac ggcttttacg aaattccggt taaggcccgg
      541 gaacagttta agaattgcaa cgttattgtc aaatttccgg tggactttga gtttccggat
      601 tttttccggg taacggatta tgccggtatt atatgtgatg tgcagtcctg cttctgccag
      661 caggggataa ttcggggagt tgaatgcatc aaaatgctct gcgctgaact tggtggtccg
      721 gttgccccgt gacagctcgc tgtcaaaata gatacaaact tcaggaacaa tgggattacc
      781 attctttttt gcggcagcaa tttctattgc cgtgataagg ttttcccttc catcggttct
      841 gggcagtccg ataggcagct gtgatcccgt gaagatcacc ggctttgaga ggttttccag
      901 cataaaactc agagctgatg cggaataggc catcgtgtcg gtaccatgca gaacgacaaa
      961 tccgtcgaag cggtcatagt tttcctcaat gatgccggcc agcctgatcc agatatccgg
     1021 atcgatattt gatgaatcct ttaccgggtc aaaggaaaca gagtgcagct cgaatccgta
     1081 gttgccgatt accggcacat gatcggttat atgcttgaaa tcgatgggaa tcagtgaacc
     1141 tgtctccgga tcatggacca tgccgatggt tcccccggta tagattatca ggactgaagt
     1201 ctcgtttttc cggtcagtca tagggtaaca ggttaaaaag tctgcatgaa ttctcaaatg
     1261 tgattgaagc ggtctcttcc tcactcattc cgaggacctc tgcaagtttt ttgtttataa
     1321 tcggaatata ggagctctca ttccgtttac cccggtaggg tacaggggcc agatagggag
     1381 aatcagtttc aagtatgata tgccggggtc cgatctccct gcaaatatcc ccgagcctgg
     1441 agttcttata ggtcaacgga ccgccgatac caagaaaaag gcccatactc acggcttttt
     1501 ccgcataact gaggccggca gagaaagcat gcagcacccc cctgaggcct gatgcggtgt
     1561 attcctccat aacagacaat acttcatcaa tggagttccg ggaatggaca accacgggaa
     1621 gaccgctttt taccgcaagg tccagctgcc ttctgaatgc cgccacctgt tcggcaaacc
     1681 ggcttttatc ccagtaaagg tcaataccta tttcgccgac tgcaataaat gcgtgttgtg
     1741 gcaggatttt ttccatccgg tcaagctgat ctgtatagtc atccccgact gatgtcggat
     1801 gaagtcccat cataggcagg catattcccc ggtattcctc agctgcctta agcatcggtt
     1861 ccactgaatg aatgtcaata tttggcataa gcagcctgac aacgcccctg cctgctgacc
     1921 tgccgatcat ttcatccctg tcgttcctga aagccggaag atataaatgt gtatgtgtat
     1981 caatgagttt catcaggcaa tttctggtat gatcatgtca ttcccggttt tccccggcag
     2041 agccctggaa aaatcacaat gcaatacggt agatattacc ggggcagttt tttatcagtc
     2101 ctttcagttc caactgcaga agtatggcgg acagtttgaa aacaggaaga tccagtgacc
     2161 ggcatattga atcgatcgtc atttccgacc cggctgacag aagatcgtaa atggattttt
     2221 ccgtttcacc gagttccggg aaaagcttct tctgctgagg atccccggtt ttttcaggct
     2281 gccagttgag aaaatattta acatccccgg ctgattcaac gagtgcggcc ctgttgtatt
     2341 tgataaggct gttgcagcct gcggaccaga tatctccggg ccgtcccggc actgcaagaa
     2401 catccctgtt ataggatcct gctatatccg cagttatgag tgcccctccc tttttccccg
     2461 attcaatgat cagggtggca tcggaaatac ctgcaattat cctgtttctt tttatgaaat
     2521 tgtttctttc cggaagggta tccgacaaga aatcagtgag gagtcctccg ttttctgcaa
     2581 ttttaccggc cagcggcctg tgaacggaag ggtatattgt gttcagaccg tgcccgagca
     2641 ctgctattgt cggaagatta ttggcaagtg ccgccttatg ggatgtaata tcaataccat
     2701 aggcaaggcc gctcactatt gtcaggccgg ggaaatctgc agccaggtcc ccgatgattt
     2761 tttcgcagat ttccctgccg cgtgaggttg aattcctggt tccgacaaca ctcaggattc
     2821 tgggtgcatc tgggtcacag ctgcctttca ggaaaaacat taccggtgaa tcctcacact
     2881 ggcggaggcg tgagggataa tcatcatcga ggtagaaata tgttctgata ttgtttcttg
     2941 tgacatacag tgcttcctgt tcagcctttt ccagatatga gcgttccgtt atgcatttgg
     3001 caagctcact gccgattccg ggaattctta tgaggtttct gtatgactcg ctgaatactg
     3061 cctccacgct ccccaggtgt gaaacaagct tccgggcgtt aatatctcct atgcgcggaa
     3121 tgataccaag ggcaattttg tgtttaagag acagattttt aggcatggca taagcggcag
     3181 gagtttagac aatacccata tccagcatgg agtttgccac tgtcagaaat cctgagatat
     3241 tggcaccttt tacgtaatca atgaatccgt cgggttgtgt gccgtactta aggcattgtt
     3301 catggatgtt gtgcattatt tcatgcagcc tttgatccac ttcctgccgg gtccatctca
     3361 ttttcattgc attctgtgcc atttccagtc cggaagtggc aaccccgcct gcattgactg
     3421 ccttgccggg agcaaagatg acgtcattgt catagaatac ttcgattgct tcgggagtac
     3481 agcccatgtt tgatacttct gcaaccatca tcacaccgtt tccgacaagt tttttcgcat
     3541 cctcagcgtt cagttcattc tgggttgcac acggaagggc aatgtcaacc tttacttccc
     3601 agggtttttt gcccggatgg aattcaacgc catcgaacat ttctgcatac ggactgacta
     3661 tatcctggtt tgatgcacgg agttcaagca tgtattcgat tttcctgccc gagaccccct
     3721 gtgggtcata gatatagccg tcgggaccgg agatggtgac aactttggct cccagctgtg
     3781 ttgccttgag ggcgacaccc caggccacgt ttccaaaacc ggaaatggaa attactttgc
     3841 cttcaatggt ttctttcctg attgccagca tttcctgaac aaaatagatg gcaccgaatc
     3901 ctgttgcttc gggtcttatc agggaacctc cccagttcag tcccttgcct gtaagtacac
     3961 cggtatgggc tcccttgagt ctcttgtaca ttccgtacag ataacctatt tcccgtccgc
     4021 ttacgcctat gtctcctgca ggcacatccg tttcaggacc gatatatttc catagttcca
     4081 gcatgaatga ctggcagaat ctcatcactt ccgcatttga ctttcccttc ggactgaagt
     4141 cggagcctcc tttgccggcg ccaaggggaa gggaagtcag ggcattcttg aaaatctgtt
     4201 caaatccgag aaatttaaga atcgactgat tcacgctctg atggaagcgg aggcctccct
     4261 tatagggtcc tatcgcacag ttaaactgta tcctgtatcc aagattcact cttacgtttc
     4321 cctcatcatc ggtccatggt accttgaaaa taagcacacg gtcgggttca ataagccgtt
     4381 caatgatatt ggctgaccgg aactgtggat tttcattgta aatatccact atggattcaa
     4441 gcacctctct tacagcctga agatattcaa cctcgcccgg atgtttcttt tcgaggtcat
     4501 tcataatttt atcaacgttc attgcaatgg ttgtttaatt agtttatggt tttgttcctt
     4561 catttatttt cccggcgata aagcctgaag ccggcaaaat taaaaaaaat cctgagccgt
     4621 cttatcagga tttgggaaga agtaaatcaa gagcactgaa taatctgttt ttctgttcac
     4681 ggttcagtgc actgaggtgg accggcgggt atttggccgt tccctggctg attttctgat
     4741 agagtattat gctttgttta aggccaaaaa gtccggattc ggttttatat cccgcgaaag
     4801 tgtttttgtg tatccggatt gagttctttt taccacctcc cattcccgcg ttgaaatatc
     4861 tgaggacaat gttttcatcg tcatcggagt agtaaataaa ctgatagtta agtataaacg
     4921 gagtgaagaa tatgatcagg taacatgaaa caagaatgat tgtccaggca gaatcgctta
     4981 gtcccagaac gggaaacctt atgattttcc ctatgtaaac aagggcaaga tatatcagga
     5041 tcaggaccgt agcgaagaaa agttttaaac ggatactgat gatctgtttc gtattgtcga
     5101 atgtcattcc gcactaattt tttataaact taatcttttt ttcttaaaga acctcagcat
     5161 aatttcccgg cattcctctt caagtattcc gcctgtcacc tttgttttgg gatgaagcaa
     5221 cctgtctgat atcagtgtat accccttttt ttcatccgga gccccgtaaa ccagtctgcc
     5281 cgcatggctc cagcccagtg cggcggcaca catggcacat ggctcaatgc ttacatatac
     5341 ggtgcagtct gtgaggtatt ttccgcccag ccagccggcg gctgctgtta ttgcctgcat
     5401 ctccgcatgg gctgtaggat cgcggagtgt ttcaataaga ttatgtgcac gggctataat
     5461 gattccattg catacaacca cggctcccac gggcacttcg tccttctcaa aggctttccg
     5521 ggcctcctcc agtgcagcct tcatgaaata ttcatgtgta tttgctttca tttgtcctat
     5581 aatcccaaag tctgacaatt ccggtatatc tgatataatc aataaaatta tcaaaattca
     5641 gtcaatgctc aaattttctt atttttgctg agatttttta aaaggggtga atatgtaccg
     5701 gacacacaca tgcggagagc tccggaccgg ggatgccggg aaagaggtta ctttgtgcgg
     5761 atggatccag acttcgaggg atcttggcgg aatgacattt attgatctcc gcgaccgtta
     5821 tgggataacc cagcttgttt tcaacatgga gaccaatccc ggcttatgtg agcaggccag
     5881 gaagctcggg agggaatttg tgattcaggt tacgggaaat gtccgggaaa ggagcaataa
     5941 aaacctgagg atacctaccg gggagataga gattgaggct gcccggtttg ttgtgctgaa
     6001 tccgagtgaa cttcctccgt tcacaattga agaggatacc gatggagggg atgaactgag
     6061 gatgaagtac cgttatctgg atctccgcag gccttccgtc cgcgataata tgatactgcg
     6121 acacaggatg gcaagagagg tgcgtaacta tatggactca gcagggttta ttgaaattga
     6181 aacacccttt ctcatcaaat cgactcctga gggggcccgt gatttcattg ttccgagccg
     6241 tttgcagccg ggcagtttct atgcacttcc ccagtcgccc cagactctga agcagctgct
     6301 gatggtttca ggatttgacc gttatttcca gattgtccgc tgtttccggg atgaggacct
     6361 gagagccgac cgtcagccgg aattcaccca gatagactgt gaaatgtcat ttgtgaccag
     6421 ggatgatatc ctggatactt ttgagggact tgtacgtcat ctgttcaaaa gcgtgaaggg
     6481 tattgaattc agtgaaccct tcataaggct cacatatgat gaggcaatgg agtcatatgg
     6541 caccgacaag cctgatctgc gtttcggaat ggaaatcaga gatctgaccg gaatagtgaa
     6601 gggacgcggt ttttcactat ttgataattc cggatatatt ggtgctataa gtgttcccgg
     6661 atgttccggc ttttccagga aacagctgga tgaactgacg ggattcgtaa caaggcagca
     6721 aagcggggca aagggaatgg tatggctgaa atacgaagcg gacggcacac tcaggtcatc
     6781 cgttgacaag ttctattccg ctgaagatct catgttatgg gtggagaaac tgcaatcagg
     6841 caggggagat ctggtattgg tgcttttcgg tgaccgggaa aagatacttc cggcattgtc
     6901 cggactcagg cttgaaatgg ggaacaggct gaatctgagg aagagagatg aatacaaacc
     6961 actgtgggtt gttgattttc cattatttga atggaatgag gaggaaacgc gtttttatgc
     7021 aatgcatcat cccttcactt ctcccagacc cggggatatt cagctgatgg aaacggatcc
     7081 gggggcggta agggccgatg catatgacct ggttataaac gggattgaga ctggcggtgg
     7141 atctgtgaga atacatgatg cctcccttca gaaactgatg tttaaaacac ttggttttac
     7201 ggaaaaagaa gcggcagagc agtttggttt cattatgaat gctttccgtt acggtgctcc
     7261 gcctcacggc ggaatagcat tcgggtttga caggctggtt gccctgttcg gcggacagga
     7321 ctcaatccgt gatttcattg cttttccgaa gaataattcg ggaagggatg tgatgcttga
     7381 cacaccggct cccgtgtcgg aaaaacagct tcaggaactg atgctgaaac cctccgggcg
     7441 gaaacagggg caggaataag gctatttccc tggttttttt gtacctatca gtctgatcag
     7501 aataactccg ttggctccct ttaccccgta tattgatgcg gacgggccct tcagcacctc
     7561 tatggattcc accattacag gctgtatttc atcaattgaa ggaacctcca tcccgtcaac
     7621 tatgaacagt ggctcggtac tcagattgaa ggaagaaggt ccgccgatga ttatctttct
     7681 gtccttgacc tgaacgccgg gcacagtacc ctttaataca tcatagatat tgttgtagga
     7741 agcatatttg gaactcacgt catttacact cgtgacgggt gtgataagct ttttcctgtc
     7801 caccgttcca tagccgatat ctatctgctc atcggaacgc gtctgagtct ccggcagagg
     7861 aaatcctacg gcgccgttaa gctttattga gagaacattc tcaccgtcaa tcactgcctc
     7921 acccagaccg gccatggtat gcaaggccga tataatccct gcatcaggct ttaccttaac
     7981 ccggaaaaat ccgtttttgt cgcttaccct gtcagtggac ttattgtcaa caaatattat
     8041 tgcaccttcg gcaggaattc ccctggcatc ggtaacaaca ccgctgacaa agaatttttt
     8101 agcttttttt tgtccggcca gaccctgtat gatgaacatt gacaacaaag cggtcaatat
     8161 cagcctgcag gccgtaaccc tgtttattaa tgtcttcatg tgtggtatat tacatatttc
     8221 tgatttattt acccttgccg gcagccgcat ttcttgccta attatgcagg cgaatccggt
     8281 gacgggtaat tactgcaagt taattcaaaa tcattacata tcctcaaaat gcctccttaa
     8341 atgatcctgc ccttaagggt tgccgaggtg tatttttcaa tgaggacatt tacataatcc
     8401 cctcttttcc tgtagtcgcc gggaaatacc acaaccttgt tttgcg
//
LOCUS       DAOSPX010000118         8386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8037_length_8386_cov_4.985356, whole genome shotgun sequence.
ACCESSION   DAOSPX010000118 DAOSPX010000000
VERSION     DAOSPX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8037_length_8386_cov_4.985356"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLZ75_07185"
     CDS             complement(<1..559)
                     /locus_tag="PLZ75_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72215.1"
                     /translation="MKPELRKISGISPDQSLVLITGKNEIPERISLSQAEREYALKQL
                     EAGEGRVFINSYFKCTYIVRIKDGLSGYRAREELRKSAAGLKKLIKSNNHKELVITSG
                     NTYREAVADFAEGLILSIYSFSKYKTKAEDEETGKNYPLSLLLEDSITKSELKWLDAL
                     TDAVYLARDLINEPVNRLNTAGLASE"
     gene            638..1726
                     /locus_tag="PLZ75_07190"
     CDS             638..1726
                     /locus_tag="PLZ75_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOS72216.1"
                     /translation="MQRDQIYNKALGLQPLDIEEGTGLYENAPLGELMFIASRLRQIH
                     NPGRKAGWMIDRNVNISNICISRCAFCNFCRRKDSPDSYVTSMDQYRQKTDELFRIGG
                     DQLLLQGGMNPALGLDFYCSLFRELKKLYPSLKLHALGPPEIVFLAKKEKTTISRVLA
                     DLIEAGLDSLPGAGAEILSDRVRKIISPAKASADEWLEVMRIAHRFNLPTSATMMYGH
                     VETVRERIEHLVRLREVQEEKPVNSYGFIAFIPWPFQDKDTRLRNKLGIKSTYTPPEY
                     LRMIAISRIMLTNIRNIQASVLTVGRETGMLSLHAGANDLGSVMMEENVVSSAGSDNR
                     FSADDLREIISAAGFEPQRRNQKYEEVK"
     gene            complement(1801..1953)
                     /gene="tatA"
                     /locus_tag="PLZ75_07195"
     CDS             complement(1801..1953)
                     /gene="tatA"
                     /locus_tag="PLZ75_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOS72217.1"
                     /translation="MGKIGATEIILILLVVVLLFGGRKIPELMKGIGQGMKEFKKASK
                     VDPKEE"
     gene            complement(2017..3354)
                     /gene="rseP"
                     /locus_tag="PLZ75_07200"
     CDS             complement(2017..3354)
                     /gene="rseP"
                     /locus_tag="PLZ75_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOS72218.1"
                     /translation="MTVLIKILQFIVSLSILVIIHELGHFLFAKLFKTRVEKFYLFFD
                     PWFSLFKFRRGETEYGIGWVPLGGYVKISGMIDESMDTEQMKQPPQPWEFRSKKSWQR
                     LLIMTGGVLFNFAFALVIYVMVLYSWGETYLPTSSVKYGIVTDSTGYRMGLRNGDKII
                     SVDNQHIENFSQILADILLNDRKTIQVERDGENLDIEVTKEYIQRVLKGEGMIEPRTP
                     FGPFVVESFARESPARSAGIQAHDELIGLDGHEFEWYDEFQAYLAAHRSEPVTMNMLR
                     NGEPVDVMVTPTAAGFLGIVRNWSVDKVFELKKINYGFFESVPAGIKKGFNTMGDYLK
                     QFRLIFSKETKAYESLGGFITIGSIFPGVWDWQSFWTLTAFLSIILAIMNILPIPALD
                     GGHVMFLIFEVISGRKPSDRFLEYAQIAGMALLFALLIYANGNDVVKLLRKFF"
     gene            complement(3351..4520)
                     /locus_tag="PLZ75_07205"
     CDS             complement(3351..4520)
                     /locus_tag="PLZ75_07205"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOS72219.1"
                     /translation="MPQKVTILGSTGSIGTQALDVISKYPGLFRVDVLTAGDNAGLLA
                     EQARKFRPATVVIGNEGHYPVLKELLKDLGIKVHAGNKAIEDMAGEAEADIILAAIAG
                     YSGLRPTIAAVRSGKKVALANKETLVIAGALVNELMKDSRDGLIPVDSEHSAIYQCLA
                     GEGISSVERVTLTASGGPFLNTPLEKMAGVTPADALKHPNWNMGDKITIDSATMMNKG
                     LEVIEARWLFNLSPEQIRVIIHPQSVIHSFVHFTDGSVKAQLGLPDMRLPILYALGHP
                     HRIKSDLPRLEFKKCPSFTFHDPDPRKFRSLDLAYHALKEGGNMPCIMNAANETAVSA
                     FLGGRLGFLQIAEVVEHTLEKGILIRDPGLEELEESDRQARLISETYIGKLTDRT"
     gene            complement(4538..5404)
                     /locus_tag="PLZ75_07210"
     CDS             complement(4538..5404)
                     /locus_tag="PLZ75_07210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS72220.1"
                     /translation="MSRNDSRKKRNWRDKYRLTVYNDTTFEEVWKIILTKYNAFLLVT
                     AVILFIIAVTVSLLAFTNLREMIPGYPDITMRRNILMSAIRLDSLEHELKLRDKYFAN
                     LNAIISGNEPVGVFSAQDTGTSYREITFRKTPEDSALRAQVESEEKYNLSVSQPVSEN
                     VTSFSGLHFFPPVKGIISGRYDPRTRHFGTDIVTRPKSSVAAVLDGTVIFTGWTMETG
                     FVIQLQHVNNIVSIYKHNANILKETGDIVRAGEPISIIGDSGELYTSGPHLHFEIWYK
                     GAPLDPEKHILF"
     gene            complement(5538..6839)
                     /locus_tag="PLZ75_07215"
     CDS             complement(5538..6839)
                     /locus_tag="PLZ75_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOS72221.1"
                     /translation="MKNRIKIPALSVLLMIFIGFACKKESVPEVITYEIRNVGTTFAR
                     SGGQVTDEGSASVTARGVCWSTGTNPTVSDSKSNDGTGPGTFVSEISGLLPATVYYVR
                     AYAINSIGTGYGDVISFKTYGDAPVSTSLPPLFVSSNESAPAASVNTFFLDTEISFEY
                     GETTAYGNSVSYSGRPLANEEKVAVKISGLKPETTYHFRVKAVNSLGTSYGEDLTFTT
                     TRPLYDIDGNSYNTVTIGQQTWTSRNLETTRFNDGTEIPLISDNSIWAELGTPGYCWY
                     NNDALLSEDSYGALYNWYAVGRGDLCPSGYHVPEYQEWIELMNFLNENQYYFVGGRGM
                     TLATATGWAYSSKVFSIGNNDHPEHRNMVGFSVLPAGVHDAVLQDFRLRGEYSAFWTS
                     SDSDIDAQNAVAVDFSYDQQEPHMSAFIKKTHGYSIRCLKD"
     gene            7386..8117
                     /locus_tag="PLZ75_07220"
     CDS             7386..8117
                     /locus_tag="PLZ75_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOS72222.1"
                     /translation="MKTTINPMLISFLLLAALSLNACEKDDNIVKDSDGNSYRTVKIG
                     TQTWMAENLKTTTYNDGTPIFNITMREEWATLERPGYCEYNNDESNANKYGRLYNWYA
                     VETGKLAPKGWHVPSKEDWDKLINYLIENGYNYDGTTSGNKIAKSLGDPKDWNDSGYE
                     GTLGSQDYPAMDNKTHFNALPSGIRQWEFRGQGYEGHLWSSTVCNINPSMPTAYYLLL
                     DYRLSNAIVEYNIKYSGMAVRCIKD"
     gene            8313..8384
                     /locus_tag="PLZ75_07225"
     tRNA            8313..8384
                     /locus_tag="PLZ75_07225"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8345..8347,aa:Met,seq:cat)
BASE COUNT         2305 a         1951 c         1814 g         2316 t
ORIGIN      
        1 actctgaagc cagtcctgca gtattaaggc ggtttaccgg ttcatttatc aggtcacgcg
       61 caaggtatac cgcatcagtc agggcatcaa gccatttaag ttcggattta gtgatgctgt
      121 cttccagcag caggctcagg ggataattct ttccggtctc ctcatcttca gcttttgttt
      181 tgtatttact gaaagagtaa atactcagga tcagtccttc cgcaaaatct gcaaccgcct
      241 ccctgtaggt gttcccggac gttattacaa gttctttgtg attatttgat tttatcagct
      301 ttttaaggcc tgcagccgac ttgcgaagtt cttccctggc cctgtaaccg cttaacccgt
      361 cctttatcct gacaatatag gtacatttga aataggaatt gataaaaacc cttccctctc
      421 ctgcctcaag ctgtttcagt gcgtattccc tttcagcctg actcaggctg atcctttcgg
      481 ggatttcatt tttccccgta atgagaacaa gggactggtc tggtgatatt ccggatattt
      541 ttcttaattc aggtttcatg aacgaatcag acaaattcaa ccagcaaagt taaacaaata
      601 attgattctt tttgtatcaa aaccttacct ttgccggatg caaagggatc agatctacaa
      661 taaggcactt gggcttcaac ctcttgatat tgaagaagga acaggacttt atgaaaatgc
      721 acccctcggg gaacttatgt tcatcgcgtc aaggctgagg cagattcaca atcccgggcg
      781 aaaagccggc tggatgatcg acaggaatgt caatataagc aacatctgca tttcacggtg
      841 tgccttctgc aatttctgcc ggaggaagga ttctcctgat tcatatgtta cctcaatgga
      901 ccagtacagg cagaaaacag acgaactgtt ccgtatcgga ggtgatcagc tcctactgca
      961 gggaggaatg aatcccgcac ttggtctgga cttttactgc tcgcttttca gggaattaaa
     1021 gaaactttat ccctcactga aactgcatgc ccttgggccg cctgaaattg tattcctggc
     1081 aaaaaaggaa aaaaccacca tttcgcgggt gctggctgat ctgattgaag ccggcctgga
     1141 ttctcttccc ggggcgggag ccgagatact gtctgacagg gtgagaaaaa tcatttcgcc
     1201 tgccaaggca tcggctgacg aatggctcga ggtcatgaga attgcacaca gattcaacct
     1261 tcccacttcg gcaacaatga tgtacgggca tgttgaaaca gtaagggaaa ggattgaaca
     1321 cctggtaagg ctgagggaag ttcaggagga aaagcctgta aacagctatg gctttattgc
     1381 attcattccc tggccgtttc aggacaagga tacccggctc aggaacaagc tggggataaa
     1441 aagcacatac acacctcctg aatacctgag gatgatagca ataagcagga taatgcttac
     1501 caatatcaga aatatccagg cttccgtgct tacagtcgga agggaaacgg gaatgctgtc
     1561 ccttcacgcg ggggcaaatg acctgggttc agtgatgatg gaggagaatg ttgtaagctc
     1621 agccggttcg gataaccgct tcagtgcgga tgatctgcgg gaaataataa gtgctgccgg
     1681 ttttgaacca cagagaagga atcagaaata tgaagaagtg aaataaaaca acaaaaaaag
     1741 ggccttgtat gcccttatca aattattcgg aattaccgga tctctttccg tactttctga
     1801 ttattcttct ttcggatcaa ctttggaggc tttcttgaat tccttcatcc cctgtccgat
     1861 gcctttcatt agctcaggaa tcttacgtcc gccgaaaagc agaaccacca caagcaggat
     1921 cagtataatc tccgttgcac ctatctttcc catctcttta agtattattg tttgtacaaa
     1981 gttataaatt ctttattcaa taacaagacc gcaatattaa aagaattttc tcagcagttt
     2041 gactacatcg ttgccgtttg catagatcag cagtgcgaaa agaagtgcca ttcccgcgat
     2101 ctgggcatat tcaaggaaac ggtcacttgg cttgcgtccc gatataacct caaagatgag
     2161 aaacatcaca tgtccgccgt caagagcagg tatcggaagg atattcatta tggccaggat
     2221 aattgagaga aaggccgtca gtgtccagaa tgactgccag tcccacacac cggggaaaat
     2281 actgccaatg gtgatgaacc ctccgagaga ttcataggcc tttgtctctt ttgaaaaaat
     2341 aaggcggaac tgcttgagat agtctcccat ggtattgaaa ccctttttta tacctgccgg
     2401 taccgattca aaaaaaccgt aattgatttt tttcagttca aagactttgt caacggacca
     2461 gtttcttaca atgccgagga agcctgcagc cgtcggagtg accataacgt caacaggttc
     2521 tccgttacgc agcatgttca ttgtcaccgg ctcactcctg tgggcagcca ggtaggcctg
     2581 aaattcatca taccattcaa actcatggcc gtccagaccg ataagctcat catgggcctg
     2641 aatgcctgcg ctccttgcag gtgactccct ggcaaatgac tcaaccacaa aaggaccaaa
     2701 gggtgttctt ggctctatca taccctcccc tttcagaacc ctctgaatat attcctttgt
     2761 tacctcaatg tcaaggtttt ctccatctcg ttcaacctga attgtcttgc ggtcattaag
     2821 cagaatatcc gcaagtatct gggagaaatt ttcaatatgc tgattatcaa ccgaaattat
     2881 tttatcacca ttcctcagtc ccattctgta acctgttgaa tccgttacaa ttccgtattt
     2941 cacgcttgaa gtcggcagat aggtctcgcc ccaggaatac aacaccatca cgtagattac
     3001 cagggcaaag gcaaagttga aaagcacgcc gcctgtcata atcaacagcc gctgccagct
     3061 cttttttgac ctgaactccc agggctgggg aggttgtttc atctgctccg tatccatcga
     3121 ttcgtctatc attccggaaa tctttacata tccgccaagc ggaacccagc ctatgccata
     3181 ttccgtttct cccctcctga atttgaaaag ggaaaaccag gggtcgaaga acaggtaaaa
     3241 tttttcaact cttgttttaa aaagctttgc aaagaggaaa tgtcccagtt catgtattat
     3301 cacaagaatt gaaagactca cgataaactg gagtatttta atcagtactg tcatgttctg
     3361 tctgttagtt ttcctatata tgtttcggat attaagcggg cctgcctgtc agattcttcc
     3421 agttcctcca gcccgggatc cctgatcagt atcccttttt caagcgtgtg ttcaacaact
     3481 tcggctatct gcagaaagcc cagccttccg ccgagaaatg ccgatactgc tgtttcattg
     3541 gcggcattca tgatacaggg catattgcct ccttccttca gggcatggta ggcaagatcc
     3601 agactccgga atttcctggg gtcagggtca tgaaaggtaa aggaaggaca ttttttaaat
     3661 tccagccgcg gaagatcgga ttttattctg tgtggatgtc caagggcata gagaataggc
     3721 agtctcatat cgggcaggcc aagctgcgcc tttacggaac catcggtaaa gtggacaaag
     3781 gaatgaatga cagactgggg atgtattatt accctgatct gttccggact gaggttgaaa
     3841 agccatctgg cctcaatcac ttccagtccc ttgttcatca ttgtggctga atcgatagtg
     3901 atcttgtccc ccatattcca gttgggatgc ttaagtgcgt cagccggtgt cacccctgcc
     3961 atcttttcaa gaggtgtgtt caggaaaggg cctccggaag cagtaagcgt tacccgctca
     4021 accgaactga taccttcccc ggccaggcac tggtaaatgg ccgaatgttc ggaatcaacc
     4081 ggaatcagtc cgtcgcgtga atccttcata agctcgttta caagcgcacc ggcaatgaca
     4141 agggtttcct tgtttgccag agcaaccttt ttccccgacc tgacagctgc aatggtgggc
     4201 cttaatcccg aataaccggc aattgccgcc agaataatgt cagcctccgc ttccccggcc
     4261 atgtcttcaa tagccttgtt gcccgcgtgt acctttatac cgagatcctt cagcaattct
     4321 ttcaggacag gatagtgtcc ttcatttccg atcaccaccg tcgcggggcg gaacttgcgt
     4381 gcctgttccg caaggaggcc ggcattgtca ccggctgtca ggacatccac cctgaacaat
     4441 ccgggatatt ttgatataac atcaagtgcc tgggtcccga tggatcctgt ggatcccaat
     4501 attgttactt tctgtggcat gtccgtagcg gaatgattca aaacagaatg tgtttttccg
     4561 gatcaagggg cgcacccttg taccatatct caaaatgaag gtgaggaccg gatgtataga
     4621 gttcgcccga atcgccgatt atcgaaatcg gttcccccgc acgtacaata tcaccggttt
     4681 ccttaaggat gtttgcatta tgcttgtata tggaaacaat attgttgaca tgctgcaact
     4741 gaatgacaaa tcctgtttcc attgtccatc ccgtaaatat taccgttcca tcgagaactg
     4801 ctgccactga tgatttgggc cttgttacta tatcagttcc gaaatgccgg gttctcggat
     4861 catatcttcc tgaaatgatt ccctttaccg gagggaagaa atggagaccg gaaaaactgg
     4921 tcacattttc agaaaccggc tgactcacac tcaggttata tttttcttca ctttcaacct
     4981 gtgccctcaa tgccgaatct tccggtgtct tcctgaaggt tatttccctg tatgatgtgc
     5041 ctgtgtcctg ggcactgaat acccccaccg gttcattccc ggaaataatg gcattcaggt
     5101 tggcgaaata cttgtccctc agtttcagtt catgctcaag tgagtcaaga cgtattgcgc
     5161 tcattagtat atttctcctc atcgtaatgt caggatatcc gggtatcatc tccctcaggt
     5221 ttgtaaaagc cagcagggaa accgttactg caataataaa cagtatcact gctgtaacaa
     5281 ggaggaaggc attgtatttg gtaaggatta ttttccatac ctcttcaaag gttgtgtcgt
     5341 tatatacagt aagcctgtat ttatcgcgcc agttgcgctt tttcctgctg tcgttcctgc
     5401 tcatgagatt caacattatt ggcatgcaaa gataataaag atgatcctta taatcagtat
     5461 tgaatataat aaaagccagc aggaagctga gaagtcccga ttacacaata tgaattccgg
     5521 cacgaaccgg gccggaatca gtctttcagg caccggatgg aatatccatg tgtctttttt
     5581 ataaatgcac tcatgtgggg ttcttgctga tcatagctga aatccacggc aacggcattc
     5641 tgagcatcga tatcagagtc cgaggaagtc cagaaagcac tgtattcacc tcttaagcgg
     5701 aaatcctgca ggactgcatc atgcactcct gccggaagaa ctgaaaaacc aaccatattc
     5761 ctgtgttccg gatgatcgtt attcccgatt gaaaatactt tgctgctgta ggcccacccc
     5821 gtagcagtgg ccagtgtcat cccccttcct cccacaaaat agtactgatt ctcattgaga
     5881 aagttcataa gctcaatcca ttcctgatat tccggaacat gatagcctga aggacacaga
     5941 tctcctcttc cgacagcata ccagttatag agtgctccgt acgaatcttc cgacagtaag
     6001 gcatcattat tgtaccagca gtatcccggc gtcccgagct ctgcccagat actgttatca
     6061 cttatcagag gaatttccgt tccgtcattg aacctggtgg tctccaggtt tctgctggtc
     6121 caggtctgct gcccgatggt tacggtattg tatgaatttc cgtctatgtc gtacaatggc
     6181 cgggttgtgg taaaggtaag atcctctcca taggaagttc ccagcgaatt aaccgccttg
     6241 accctgaaat gataggtagt ttcaggttta aggccggaga ttttgacagc taccttttcc
     6301 tcattggcga ggggacgtcc ggaatagctc actgagtttc cataagcagt cgtttcacca
     6361 tactcaaaac ttatttccgt atcaagaaaa aaagtgttca cactggctgc aggagcggat
     6421 tcattactgc ttacaaagag cggaggaagg gaggtactca caggtgcatc gccataggtt
     6481 ttaaaagata taacatcccc atagcctgtt ccaatgctat tgatagcata agccctgaca
     6541 taatagacag ttgcaggcaa taatcccgat atctcactga caaaagttcc cggcccggtt
     6601 ccatcattac ttttactgtc agatacagtc ggattggttc cggtactcca gcatactccc
     6661 ctggctgtaa ccgaagctga tccttcatcg gtcacctggc cacccgatct tgcaaaggta
     6721 gtccctacat tccttatttc ataggttatt acttcaggca ccgattcttt tttgcaggca
     6781 aaacctatga atatcataag caggactgac aaagccggaa tcttgattct gtttttcata
     6841 caacaatact ttgtttaaat attaaaaaaa ttatttaaat gtcactgaaa taatcaatac
     6901 ataattatga agcaattcat gcatctttct gacaatccgt aactgacaga ttaaaccaat
     6961 ccaggtcagg ttatgatcac cagggaacaa aaatatttca atgtactcat ttttagtata
     7021 actgacaaaa tcccaaatgc cgtattttcc ttttatatcc atcgccggac atgggtacaa
     7081 tgccggggtt gtgtaaggaa gcctgcttca acatgacgca aaacaatgga tttgaggacc
     7141 agtcaggctt ttcaaatttt aaaagtaagt cattcaaaat tacatggttt ctttatcttt
     7201 gattccagga taccaaagaa ctttcatgtt agatgttggt aactccctgt aataattaac
     7261 aatttgagtt taaagacaga cactgattaa taatttgtta tcctggcaaa aagaatgtct
     7321 caggccgaag ctcttaacat ggcttatttg tttcattaca ttacaactat tattgacaga
     7381 taattatgaa aaccacgatc aacccaatgc tcatttcttt tctcctgctg gctgctcttt
     7441 cgctgaacgc ttgtgaaaaa gacgataata tcgttaagga ttctgacggt aattcctatc
     7501 gtactgttaa aatcgggaca cagacctgga tggctgagaa tttaaagaca acaacataca
     7561 acgacggaac accgatattc aatataacca tgcgggagga atgggcaact ctggagagac
     7621 ccggatattg tgagtacaat aatgatgaaa gcaatgcaaa taagtatggc cgtctgtata
     7681 actggtacgc cgttgagacc ggaaaacttg caccaaaagg ctggcatgtg ccgtcgaaag
     7741 aggattggga taaactgata aactacctga ttgaaaacgg gtataattac gacggaacaa
     7801 caagcggaaa taaaatagcc aaatctcttg gagatccgaa ggactggaat gattcaggct
     7861 atgaaggcac cctgggaagt caggactatc cggctatgga caataaaaca cactttaacg
     7921 cgcttccttc aggaatccgg cagtgggagt tcagaggtca gggttatgag ggacacttat
     7981 ggagcagcac tgtttgtaat ataaacccgt ccatgcctac tgcctattat ctgttactgg
     8041 attatagact gtcgaatgca attgtcgaat ataatataaa gtatagcgga atggctgtac
     8101 gctgtatcaa agattgatat tgatgatggt tttttcaggg acggatacaa atatccggaa
     8161 agttatcaaa accggcataa tccgcagcat aaagcatatt gctttgtctg ttaatgcctt
     8221 aaactttaaa agaattaacc ggaataaatc tgattaaaaa acctgattta atttgtatat
     8281 tgaacggtaa gtattatctt tgcaccgcac attgcggggt ggagcagtgg tagctcgttg
     8341 ggctcataac ccaaaggtcg gaggttcgaa tcctccctcc gctaca
//
LOCUS       DAOSPX010000119         8376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8048_length_8376_cov_4.489244, whole genome shotgun sequence.
ACCESSION   DAOSPX010000119 DAOSPX010000000
VERSION     DAOSPX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8048_length_8376_cov_4.489244"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PLZ75_07230"
     CDS             complement(<1..1376)
                     /locus_tag="PLZ75_07230"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS72223.1"
                     /translation="MMKKATIITLVVAALAILVLVVINRTGSKKDLENLYVEARRGQF
                     DIVVTTTGELQAENSTDIRGPEFTQSRNIRAMEIKITDLVPEGTEVSEGDYIATLDRT
                     SFDNSLKDELENLITFETNLEVKMLDTAVTLSNLRDQIKNLRFSVEEAQITLQESKYE
                     PPTTIRQAEIALDKAQRSLEQAQRGYHLKVEQARSDMRTIKNRLAEQNTRVRDLQKLL
                     EQFVIYAPSEGMVIYKRDRMGAKRKVGSSISPWDNVVATLPDMSTMISKTYVNEIDVS
                     KVKTNQEVEIMVDAFPEKKYSGVVLSVANIGEQLPNADAKVFEVIVKLNESDPILRPS
                     MTTGNKIVTKTIDDVTYIPLESVQTGPDSIPFVYLKNGSKQIVVLGESNENNVVVEQG
                     IEPGVQLYLNTPKKPEEFTRLLGENLVSVIKEKERIRKEEERKASEQTERRGPGGRGG
                     GMMMNMTP"
     gene            complement(1544..2923)
                     /gene="glmM"
                     /locus_tag="PLZ75_07235"
     CDS             complement(1544..2923)
                     /gene="glmM"
                     /locus_tag="PLZ75_07235"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOS72224.1"
                     /translation="MTLIKSISGIRGTIGGRPGDNLTPVDIVKFTAAFASWIRAGGND
                     SPGIVIGRDARISGPLVSSLVSDTLRSMGINVRDLGLASTPTVEMAVAGTGADGGIIV
                     TASHNPAQWNALKLLNARGEFIGEAEGEEILRLAAAEDFEFAAAGREGRYVTDDSWNG
                     KHIDQILRLDLVDVKAIEKAGFTVAVDCVNSVGGIIVPQLLKALGIKSVIELNTTPDG
                     IFPHNPEPLPENLGEISALVADRKADLGLVVDPDVDRLAIICEDGSMFGEEYTLVAVA
                     DFILRNTPGSTVSNLSSTRALKDVTEQYGCRYFSSPVGEVHVVEEMKARNAVIGGEGN
                     GGIIYPALHYGRDALAGIALFLTHLALSGMKCSELRGTYPSYWIAKKKLELDPQSDLS
                     HILQSVRNHFSGMETDERDGLRIDLPDGWFQLRKSNTEPVMRIYAEGRSEGEARLLAE
                     RVISIVKKE"
     gene            3042..3755
                     /locus_tag="PLZ75_07240"
                     /pseudo
     CDS             3042..3755
                     /locus_tag="PLZ75_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            3761..4639
                     /gene="era"
                     /locus_tag="PLZ75_07245"
     CDS             3761..4639
                     /gene="era"
                     /locus_tag="PLZ75_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOS72225.1"
                     /translation="MKHRSGFVNILGNPNTGKSTIMNSLVGEKLSIITSKAQTTRHRI
                     MGIVNSEDFQIVYSDTPGILKPAYKLQESMMNFVKTALADADVIMYVTDVTEKAVSKT
                     EYPEKIRSSGIPVIVAVNKIDLTNQQELEKIVEEWQDFFPGSPVIPVSALNKFNLGTL
                     LNAVISRLPEGEAYFPKDQLTDKHERFFASEIIREKILGNYRKEVPYSVEIEIESFRD
                     EGRILKIRAVIHVARESQKAILIGHRGSMLKKTGTEARHDMEDFFGRKVYLELYVRVS
                     RDWRDKPAMLRKFGYL"
     gene            4655..5959
                     /gene="der"
                     /locus_tag="PLZ75_07250"
     CDS             4655..5959
                     /gene="der"
                     /locus_tag="PLZ75_07250"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOS72226.1"
                     /translation="MSRIVAIVGRPNVGKSTLFNRLTESRLAIVDETSGVTRDRNYGS
                     CTWNGIDFSLIDTGGFVEDSGDIFEEEINKQVLVAIEESDLILFMVDVTAGIHELDKA
                     IAGLLRKSGRKMLLVVNKVDNSQRLMEAAEFYELGTERYFPVSSVNGSGTGDLLDEIV
                     SILPEKEPETIPEIPRVAIVGRPNVGKSSLVNSLLGEERNIVTPIPGTTRDSIYTRYN
                     KFNHDFYLVDTAGLRKKSRVSENIEFYSVLRAVRTIENSDICLLMIDATRGMEAQDLN
                     ILSLILKNGKGLILAVNKWDLVEKDSNTARLLEKEIRKKTAPFTDFGIIFISAVKLLR
                     IHKILDLINTVNKNRTRKIGTAELNSVMLDIIRQNPPPSARGMKISIKYITQLPTYSP
                     SFAFFCNHPQYIKEPYKRYIENRMREKFEFTGVPLRLFFRKK"
     gene            6006..6536
                     /locus_tag="PLZ75_07255"
     CDS             6006..6536
                     /locus_tag="PLZ75_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72227.1"
                     /translation="MKTRRYLILLILAPVLAAGCQKIEHLPPEPKIEFRYFEVFDTTD
                     ILGNKCQGGRLNFYFEDGDGNLGLPPPDGTHSDTTNLFLTLFRKTKGRMTEVGANDIL
                     RPSNYRIPYMDRQGQNKILRGTISVTFLYLFYSLESHDTIRYDFYIKDRAGNISNTES
                     TSEILLSENNTYRKTK"
     gene            complement(6567..>8376)
                     /locus_tag="PLZ75_07260"
     CDS             complement(6567..>8376)
                     /locus_tag="PLZ75_07260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOS72228.1"
                     /translation="IILDRTTGRVKARYNTNNGLPYNRIDQICLLGGGNAAIATKTDR
                     IYTVNIEDGVSSRSGIMRGPTMNVITSGFRSSDGSIWAATAGNGVFEFRGDSVISYTR
                     AEGLLSDYCYSILADSLNRIWIGHERGFSCYDRLSGSMKTYSTGFARGGACNPGAMHE
                     SPGGRVLIGTTQGLVVYDLDKDCKTGQAPVNNINYISVNDVRYPYRKSFSLPYKKRYR
                     INIGYVGISFREPEKVYYQTRLDNWDDDWSAWSTDREVTVSPRDGRFRFSMNSVNEEG
                     LSGQAVTFDLSIRTPFWRTWWFFILSAAAASGIVVLIIRQREKAQKRLELYLKTELEA
                     RTEEVVRQKGEIELKNVEITDSISYAKRIQTSILPDLNKLKETFSDAFILFRPRDIVS
                     GDFYWFDRFGDDRFMLVCADSTGHGVPGAFMSMIGSTLLQDIIGRQHITRPSVILKTL
                     DSQLFSTLNQNIELGVSNDGMDMVVCEIGLRTGHIRFASAMRPVIMILGGEFLYVKGN
                     RSSIGGESLAEKFFDDQEYYLKEGDSIYLFTDGYPDQFGGTEGKKMKIARLKSLIEEV
                     SNLSMDEQREKIESFLDEWQGENDQVDDILMMGIRF"
BASE COUNT         2267 a         2116 c         1841 g         2152 t
ORIGIN      
        1 tccggagtca tattcatcat catgccgcct ccccggcctc caggccctct tctctctgtc
       61 tgctcactgg ccttccgctc ctcttccttc cttatcctct ccttttcctt aattacgctt
      121 accaggtttt ccccgagaag cctggtgaat tcctcaggct ttttgggcgt gttgagataa
      181 agctgtactc cgggttcaat cccttgttcc acaacaacat tattttcatt cgattctcca
      241 aggaccacta tctgctttga gccgtttttc agatagacga aaggaatgct gtcaggtccg
      301 gtctgtacac tttccagagg tatataggtc acatcatcaa ttgtttttgt aacaatcttg
      361 ttcccggttg tcattgacgg cctcagaatc gggtccgact cattaagctt gacaatcact
      421 tcaaaaacct ttgcatcggc attgggaagc tgttcaccta tattggcgac acttaaaaca
      481 actcccgaat atttcttttc cggaaatgca tctaccatga tttccacttc ctgatttgtc
      541 ttcaccttgc taacatcaat ctcattgaca taggtttttg agatcatggt tgacatgtcg
      601 ggaagggtgg caacaacatt gtcccacgga cttattgatg agcccacttt cctttttgca
      661 cccatcctgt cgcgtttata gatgaccata ccttccgagg gcgcatagat cacaaactgt
      721 tccaggagct tctgcaggtc cctgactctc gtgttctgtt cagcaagccg gttcttgatt
      781 gttctcatat cggaacgtgc ctgttccact ttcagatggt aacccctttg tgcctgttca
      841 agcgacctct gggccttgtc aagggctatt tcagcctgcc ggatggtagt cggaggctca
      901 tatttggatt cctgaagggt aatctgagct tcctcaacac tgaaacgcag attcttgatc
      961 tgatccctga gattactgag agttaccgct gtatcaagca ttttaacctc caggtttgtt
     1021 tcaaaagtga taaggttctc aagctcatcc ttgaggctgt tatcgaatga agtcctgtca
     1081 agcgttgcaa tataatcacc ttcactgact tcagtgcctt ccggaaccag gtcggtgatc
     1141 tttatttcca ttgcccttat attcctgctc tgagtgaatt caggcccgcg tatatcggtg
     1201 gaattctcgg cctgtaattc ccctgtggtg gtaacaacaa tgtcgaactg ccccctccgg
     1261 gcttccacat acagattctc cagatccttt ttggatccgg tcctgttgat cacaaccagc
     1321 acaaggatcg ccaaagccgc gacaacaagc gtgataatgg ttgccttttt catcattgat
     1381 ttattttata aacagtcata aaaaatattc aaaaggattc gcgaattgtt ccggcccgga
     1441 aaacccggtg ctgaatcttt catttctcca ttccgggcat aatatccggg gaacaccaat
     1501 aagacattcc ggcagccggt tttattcctc cgtccgtaaa tatttattct tttttaacaa
     1561 ttgaaattac tctttcagcc agcagccggg cctcgccttc tgatcttccc tctgcataaa
     1621 tcctcattac cggttcagta tttgacttgc gcagctgaaa ccatccgtca ggcagatcaa
     1681 ttctcaaacc atccctttca tcagtttcca tgccgctgaa atgattcctg accgactgaa
     1741 gaatatggga taaatctgac tgcggatcaa gttccagctt cttctttgct atccagtagg
     1801 aaggataggt tcccctcagc tccgaacatt tcattccgct cagggccaga tgggtcagaa
     1861 aaagggcaat acctgccagt gcgtcccttc cgtaatgaag tgcaggataa atgatgcccc
     1921 cgttgccttc tcccccgatc actgcattcc gggctttcat ttcctcaaca acatgcacct
     1981 cgccaacggg agaggagaag tacctgcatc cgtattgctc ggtcacatcc ttcagtgccc
     2041 tggtggatga caggtttgaa accgtgcttc cgggcgtgtt ccggagtatg aaatccgcaa
     2101 cggcaacaag cgtgtattcc tctccaaaca tagatccgtc ctcgcagata atcgccagac
     2161 ggtccacatc cggatcaact acaagaccga gatctgcctt tctgtctgca acaagggcag
     2221 agatttcgcc caggttttca ggaagcggtt cgggattatg ggggaagatg ccgtcgggcg
     2281 tggtgttcag ttcaatgacc gactttattc caagtgcttt cagaagctgc gggacaatta
     2341 tcccgccaac cgagttaaca cagtcaacag ccacggtaaa acctgccttt tcaatagctt
     2401 tcacatcaac caggtccagc ctgaggatct ggtctatatg cttaccgttc caggaatcgt
     2461 cagtgacata tctgccttcc cggcctgcag cagcaaactc aaaatcctct gccgcggcca
     2521 gtctcaggat ctcctcgcct tcagcctccc cgataaattc cccccttgcg ttaaggagct
     2581 tcagggcatt ccactgcgcc ggattatgac ttgctgtcac aattattcct ccgtcagccc
     2641 ccgttccggc aacagccatt tcaacggtgg gagtacttgc caggcccaga tccctgacat
     2701 ttatgcccat acttctcagg gtatctgaaa ccagtgagct cacaaggggt ccagagattc
     2761 ttgcatcacg ccctatcaca attccagggg aatcattgcc gccggccctg atccaggaag
     2821 caaacgcagc agtgaatttc actatgtcaa caggtgtcag attatcaccc ggcctgccgc
     2881 ctattgttcc cctgattccg gaaatggatt ttatcagtgt catttcttgg ttttctggcc
     2941 tgcaaatttg caaaaatttc atcaaataat aaatatatca aataatcgta cctttgcatc
     3001 acttaaaaac aaccctgatg gacaagcgga aaaagaagga agagctgatc cggcttaaca
     3061 ggtatatagc cggcagcggc ctctgttcac gccgcgaggc ggatgaatac atcacagggg
     3121 gatttataag tgtcaacgga aagacaataa cagacctggg gacgaaaata aaccgggatg
     3181 acaatgtgag atacaagggc agaaagcttc tgcccgaaaa gaaggtctat ctgctgatca
     3241 acaagcctgc cggatatgtt accaccatga aggaccctca tgctgaacga acggtgactg
     3301 atctggtaag gaatgcctgt tcagaaagga tttatccggt gggaaggctt gacaaggata
     3361 caacaggtgt ccttctcatg acaaatgacg gtgaccttac aaaaaagctg actcacccga
     3421 aatttgaaag aaaaaaaata tatcacgtcc atctcgataa accggtaagc aaggccgacc
     3481 tctacaggat tgttgaggga ataagcgtgg atggctcccc ggttccggtg gattctgtgg
     3541 aatatgctga tccggacgac aggaagcaga taggtattga actccattcc gggcaaaaca
     3601 gggtggtgcg gaaaatattc gaatcgctcg gatacagggt cagcaaactc gacagggtct
     3661 atttcgccgg tctcacaaaa aagaatctcc ccaggggaaa atggagattc cttacacaaa
     3721 aggagattgc catgcttaaa agaggaattt attaaacgaa atgaaacacc ggtcaggttt
     3781 tgtaaatatc ctgggaaatc caaatacggg aaagtcaacc ataatgaatt cccttgtcgg
     3841 ggaaaagctt tccataataa cttccaaggc ccagaccaca cggcaccgga taatgggcat
     3901 cgtgaactcc gaagatttcc agatagtcta ttccgacacg cccggcatcc tgaaacctgc
     3961 ctacaaactg caggaatcaa tgatgaactt tgtgaaaacg gcacttgccg acgccgatgt
     4021 tataatgtac gtgactgatg ttacggaaaa ggctgtgtcc aaaacagaat accctgaaaa
     4081 gatcagatca tccggcatac ccgtaattgt tgccgtgaac aaaatagacc tgacaaacca
     4141 gcaagaactg gaaaagatag tggaggaatg gcaggatttt ttccccggct cacccgtgat
     4201 acccgtatct gcactgaaca aattcaatct cggcaccctc cttaatgcag taatatcgag
     4261 acttcccgag ggagaagcat atttccccaa ggaccagctt accgataagc atgaaagatt
     4321 ttttgcatcc gaaataataa gggaaaagat tctcggcaat taccggaaag aagttcccta
     4381 cagtgttgaa atagagattg agagcttcag ggatgaaggc agaatactga aaataagggc
     4441 cgtgattcat gtggccaggg aaagtcagaa ggccatactg ataggtcaca ggggctcaat
     4501 gcttaaaaag accggcacgg aggcaaggca tgacatggag gatttttttg gcaggaaggt
     4561 atacctcgaa ctctatgtca gggtaagcag ggactggagg gataagccgg cgatgctcag
     4621 aaagtttggt tacctataat gtaaagaatg aattgtgagc agaatagttg ccatagtagg
     4681 acgcccgaac gtgggtaagt caaccctgtt caacaggctt accgaatcaa ggcttgccat
     4741 tgttgatgaa accagcggtg ttacccgcga ccgcaactat ggcagctgca cctggaacgg
     4801 aattgatttc tccctaatcg atacaggggg ctttgtcgag gattccggcg atatattcga
     4861 ggaggaaatc aacaaacagg tgttagtggc catagaggaa tccgatctca tactcttcat
     4921 ggttgatgta actgcaggaa tacatgaact ggacaaagcc attgccggtc ttctgagaaa
     4981 atccggcagg aaaatgctgc tggtggtgaa caaggttgat aacagccaga ggctgatgga
     5041 agccgctgaa ttttacgaac tgggaactga acgctatttc cccgtcagct cagtaaacgg
     5101 cagcggcacc ggtgatcttc ttgatgaaat tgtaagcatc ctcccggaaa aagagccgga
     5161 aacaattccc gaaataccgc gggtggcaat tgtcggcaga ccgaacgtgg gaaaatcttc
     5221 actcgtgaat tcccttctag gggaggaaag aaatattgta acgcctattc cgggaacaac
     5281 ccgcgattcc atctataccc gctataataa attcaatcac gacttttatc ttgttgacac
     5341 tgcaggattg agaaaaaaaa gcagggtcag cgagaatatt gaattctatt ctgtcctgag
     5401 agcagtcaga accattgaaa attcggatat ctgcctgctc atgatcgatg caaccagagg
     5461 catggaggca caggatctca acatactgtc gctgatactc aagaacggca agggacttat
     5521 cctggctgtg aataaatggg atcttgttga aaaagacagt aatacggcac gactgctgga
     5581 aaaggaaatc aggaaaaaaa ctgctccctt tacggatttc gggataatat ttatttcggc
     5641 agtgaaactg ctcaggatcc ataagatact tgatctgatt aacactgtca acaaaaacag
     5701 aaccaggaaa attggcacgg cagagctcaa ttcggtaatg cttgacatta tcaggcaaaa
     5761 tcctccgcca tcggcaagag gaatgaaaat cagcataaaa tacataacac agctgccgac
     5821 atattcaccc tcgtttgcct ttttctgcaa ccatccccaa tacataaagg aaccatataa
     5881 acgttatatt gaaaacagga tgcgtgagaa atttgagttt acaggtgtac cgctcagatt
     5941 atttttcaga aagaaataat cacctttggt gtgatttttg ttcaggaaca atcaggcgct
     6001 gaattatgaa aacacgaaga taccttattc tgcttatcct ggctccggta cttgctgccg
     6061 gctgccagaa gattgaacac ctcccgccgg agccgaaaat tgaattccgc tattttgaag
     6121 tttttgacac aaccgacatt cttggaaaca aatgtcaggg aggcagactc aatttctatt
     6181 tcgaggatgg cgatggcaat ctgggattac ctccgcccga cggaacccat tccgatacca
     6241 ccaacctttt tctcacactt ttcagaaaaa caaagggccg gatgaccgaa gtcggagcca
     6301 atgacatact caggccctcc aattacagga taccatatat ggacaggcag ggacagaaca
     6361 aaattctcag gggaaccata tcggtcacct tcctgtacct cttttattca cttgaatccc
     6421 acgatacaat caggtacgat ttctacataa aggacagggc cggtaacatc agcaacaccg
     6481 aatccacaag cgaaatctta ctttccgaaa ataataccta cagaaaaacc aaatgaactg
     6541 atccgccggc agaatcagta tatacatcag aaccttattc ccatcatcag gatatcatcc
     6601 acctggtcat tctccccctg ccattcatcc aggaaactct caatcttttc cctctgttca
     6661 tccattgaga gattacttac ttcctcaata agcgatttaa ggcgtgctat cttcatcttt
     6721 ttcccttccg tcccgccaaa ctggtcgggg taaccatcgg tgaaaagata gatactgtcc
     6781 ccttccttaa ggtaatattc ctgatcatca aagaatttct ccgccaagga ttccccgccg
     6841 atcgacgatc tgttaccctt cacatacagg aactccccgc cgagtatcat tatcaccggc
     6901 cgcattgcgg aggcaaatct gatatgaccg gtcctgagac ctatctcaca tacaaccatg
     6961 tccatgccat cgtttgacac cccaagctca atattctggt ttaacgttga aaaaagctga
     7021 ctgtcaagtg tcttcaggat aaccgaaggc ctggttatgt gctgccttcc tataatgtcc
     7081 tgaagaaggg tggagcctat catcgacatg aaggcacccg gaacgccatg acccgttgaa
     7141 tctgcacata ccagcatgaa tctgtcgtca ccgaacctgt cgaaccagta aaaatccccg
     7201 ctcactatat ccctgggtct gaaaaggata aatgcatcac tgaaagtttc cttcagcttg
     7261 ttcagatcgg gcagtatact ggtctgtatc ctcttggcat agctgatgct gtcggttatt
     7321 tcaacattct taagttctat ttcgcctttc tgcctgacaa cctcttcggt cctggcttca
     7381 agttcagtct tgagatacag ttccagcctc ttctgggctt tttcgcgctg ccgtataata
     7441 aggaccacaa tgcctgaagc agctgctgcc gaaagaataa aaaaccacca ggttctccag
     7501 aacggagttc tgatcgaaag gtcgaatgtt acagcctgtc cggataatcc ttcctcattc
     7561 acggaattca tgctgaacct gaatctgccg tcgcgcgggg aaacagtcac ttccctgtcg
     7621 gtgctccatg ccgaccagtc gtcatcccag ttatcaagcc tggtctggta atataccttt
     7681 tcaggttccc tgaaactgat tcccacataa ccgatgttaa tgcgatagcg ttttttatag
     7741 ggcaggctga atgatttcct gtaaggatat ctgacatcat tcactgaaat gtaattgata
     7801 ttatttacgg gggcctgtcc ggttttgcaa tccttatcaa ggtcataaac caccaggccc
     7861 tgtgtggttc caatcagcac ccttcctcca ggggactcat gcatggcgcc ggggttgcag
     7921 gcacctcccc tggcaaaacc tgtggaataa gtcttcatgc tgcccgacag cctgtcgtag
     7981 caggaaaatc ccctttcatg cccgatccat atcctgttga gtgaatcggc aagaatgctg
     8041 tagcagtagt ctgacaaaag cccttcagcc cttgtatagg aaatgaccga atcgccccgg
     8101 aattcaaata cgccgttccc ggcggtggct gcccatatac tgccgtcact gctccggaaa
     8161 ccggatgtta tgacattcat ggtaggtccg cgcattatgc cgctccgcga tgacacaccg
     8221 tcctcaatgt ttacagtgta aatacggtct gttttggtcg ctattgctgc attgccgccg
     8281 ccgagaagac agatctggtc tatcctgtta tagggcaggc cgttgtttgt gttatatctt
     8341 gccttcaccc ttcccgtggt ccggtcgaga atgatt
//
LOCUS       DAOSPX010000120         8366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8063_length_8366_cov_4.313681, whole genome shotgun sequence.
ACCESSION   DAOSPX010000120 DAOSPX010000000
VERSION     DAOSPX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8063_length_8366_cov_4.313681"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /gene="ispG"
                     /locus_tag="PLZ75_07265"
     CDS             complement(<1..1186)
                     /gene="ispG"
                     /locus_tag="PLZ75_07265"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOS72229.1"
                     /translation="MTNAGNNRETPRKSASVLIGRLPLGGHYPVRIQSMTNTDTLDTK
                     SSVAQCIRIIEAGADMVRLAAQGIKEARNLENIKREVRKAGFSTPLIADIHFNPAAAE
                     TAARIVEKVRINPGNYADRRASFIRNELSEKEYNEELERIHERILPLINICRENDTAI
                     RVGINHGSLSDRIMTRYGNTPEGMVQSALEFINIFRSENFTRLVLSVKSSDTGVLLES
                     NRLLVKRMQENGMIFPVHLGVTEAGEGEDGRVRSAAGTGALLSEGIGDTIRVSLSEDP
                     EKEIPVAREIVDFLCGPRGRIRTPVPAQDFVTRERLWKPEVITYNEGRYLKEDNSPFS
                     GRMLIFNFNSPPLLSGRPDAGDYLNPVFDEDDPEKLVIRASALLGRYFILRQPEGICI
                     SNR"
     gene            complement(1183..2652)
                     /gene="cysS"
                     /locus_tag="PLZ75_07270"
     CDS             complement(1183..2652)
                     /gene="cysS"
                     /locus_tag="PLZ75_07270"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOS72230.1"
                     /translation="MQNNLYIYNSLTREMELFEPLHPPFAGLYVCGPTVYSDAHLGHA
                     RPAITFDLLFRYLTHLGYKVRYVRNITDVGHLEDDSDDGEDKIGKKARLEHLEPMELV
                     QKYMNNFLKNMEELNTLPPSIEPRASGHIIEQVEMIKKIIDAGYAYEANGSVYFDVPK
                     YNEKFSYGKLSGRVLDELQQNTRTLEGSSEKRSPWDFALWKKAAPGHIMRWPAPWSTG
                     FPGWHLECSAMSTKYLGEVFDIHGGGIDLQFPHHECEIAQSTAALGKESVRYWMHNNL
                     ITINGQKMARSLGNFITLDQLFSGDHPLLQQAYSPMTIRFFILQAHYRSTVDFSNEAL
                     SAAGKGLRRLMKAAAALRNLKPSKDSSLDIASVGRGYYEAMNDDLNTPLLLSRLFDSI
                     RYINSVNEGTDRISGSDLETFRSLFSTFVFDILGLKNESADRDDEKLTSDLMKIIIDL
                     RQDARNRKEWEVSDHIREELKKAGIVLNDTREGMTWSRE"
     gene            2923..6573
                     /gene="metH"
                     /locus_tag="PLZ75_07275"
     CDS             2923..6573
                     /gene="metH"
                     /locus_tag="PLZ75_07275"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOS72231.1"
                     /translation="MKYSPEDIITRKILILDGAMGTMIQKQGLNEDSYRGSLFGDHPV
                     PLKGNYDILNITSPDVIRKIHLDYLEAGADIISTNTFNANAVSQADYRMEDQVYRMNL
                     EAARIAGEAISLHEKSGGSGIHFIAGAIGPTNRTASMSSDVNDPGARQITFDELAEAY
                     SEQVRGLIDGGADILLIETVFDVLNCKAALYGISNIFEERGISLPVMVSGTITDASGR
                     TLTGQTLEAFLVSVSHFPLFSIGLNCALGAEQLRPFVQELSGKTEFYVSAHPNAGLPD
                     QFGEYNQSAGYMAMVTEEFMKEGWVNIIGGCCGTTPLHIRKIAELAEKYPPRQKPAIK
                     KYTRLSGLEVLTITPETNFVNIGERTNVSGSSKFARLIREEKYSEALAVARNQVEGGA
                     QIIDICMDEAMLDSERAMVRFVNLIMAEPDIARLPLMIDSSKWSVITSALKCIQGKSV
                     VNSISLKEGEEVFLGHAREIRKFGAAAVVMLFDEKGQADSYERKTEVAERSYRLLVEK
                     AGFPPEDIFFDPNVLAVATGIEEHNSYAVSFIRATTWIKENLPHARVSGGISNLSFSF
                     RGTDRVREAMHSVFLYHAINAGLDMAIVNPGMLQVYSEIDPDLLKLTEDVILNRRKDA
                     TERLVKFAEGLKNEGKKEEKSDEWRALPVIERIRHSLVRGLDEHIEQDVEEARPLFAR
                     SIELIEGPLMSGMNEVGDLFGSGKMFLPQVVKSARVMKRAVSRLEPYIEIESRHEEKK
                     SAGKIVLATVKGDVHDIGKNIVGVVLSCNSYQVTDLGVMASTEKIIDTALEEKADFIG
                     LSGLITPSLEEMVHVAAEMERRKLTIPLLIGGATTSEVHTAVKVSPKYSAPVIHVKDA
                     SKAAGVLSALLREDRAQYAAAVSERYASLREEHTVKRSERKLLPLADARENRLNTDWN
                     TQKPFIPLKPGYRIIDDIDLNLLTGYFDWTFFFVQWKLTGRYPAILNDPVKGPEARKL
                     YDDAQQLLKEIIERKLISAKAVFGLFPAGSEGDDVAVLSDRKGSNRTTWLRFLRNQGV
                     KETDFPNLCLADFIAPLSSGVADNIGLFVVTAGIDEDKFSMYREDDYASIMIRMLSDR
                     FAEAAAEWLHERIRKEYWGFAADENLPVEDLLAVKYRGVRPAPGYPACPDHTEKRVLF
                     DLLDAENAIGAGLTENFAMIPPSAVSGYIFSHPASTYFTVGRIGNDQLKDYAERKNMS
                     IDEAARWLAPNL"
     gene            6586..7539
                     /gene="metF"
                     /locus_tag="PLZ75_07280"
     CDS             6586..7539
                     /gene="metF"
                     /locus_tag="PLZ75_07280"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOS72232.1"
                     /translation="MSVIEKIKNAKGPLFTFELLPPLKGHSIERIYSAIDRLMEFEPA
                     YINFTSHRNETSYKERPDGLLEKRVTRLRPGTIALAAAVKYKYNITVVPHILCGGFTR
                     EETENVLIEMNFLNIDDVLALRGDPQRGSRVFIPEKDGHRYTSELVAQIVDMNNGRYL
                     EDTLRENTPTSFCIGVAGYPEKHGEAPNMETDIVNLKKKIDAGAQYIVTQMFFDNGKY
                     FRFRDLCRDAGIHVPVIPGIKPVSALNDIKMLPKTFHIDLPNDLVKEVEKCSTDQEAR
                     EVGIEWTTMQSRELISRGAPGIHYYTLGRSDNIARIVKASF"
     gene            complement(7667..>8366)
                     /locus_tag="PLZ75_07285"
     CDS             complement(7667..>8366)
                     /locus_tag="PLZ75_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOS72233.1"
                     /translation="IAADLKKYLFPGVRESLPGNVKMLLGDDVRLLVNPTGKFVIGGP
                     HGDTGLTGRKIITDTYGGHGAHGGGAFSGKDPSKVDRSAAYAARHIAKNMVAAGVCSQ
                     ALIQVAYAIGIAQPVGFFVNTFGTARVKDSSGRILTDGEIASRVRKLFDLRPYFIIER
                     LGLKNPVFLKTASYGHFGKEPHIGNVEVYYEVPGSIPRILNNNGKKGWMKEVEFFAWE
                     KLDYVDKIRNEFGI"
BASE COUNT         2130 a         1985 c         2205 g         2046 t
ORIGIN      
        1 ccctgttgct tatacaaatg ccctcaggct gcctgagaat gaaatagcgg cccagcaaag
       61 ccgaggcacg gataacaagc ttctccggat cgtcttcatc gaagacagga ttgagataat
      121 cgcctgcatc gggcctgcct gagaggagag gaggggagtt gaagttgaaa ataagcatcc
      181 ttccggaaaa cggactgttg tcctctttca gataacgccc ttcattgtaa gtgattacct
      241 cgggtttcca gagcctttcc cttgttacaa aatcctgggc cggaacggga gtcctgatcc
      301 gtcccctggg accgcagaga aagtccacta tttcccttgc cacaggtatt tccttttccg
      361 gatcctctga cagcgatacc ctgatggtat cgcctattcc ttcagacagc aatgctcctg
      421 ttcctgcagc cgatctcacc cttccgtctt ctccctcacc ggcctcggtt actcccaggt
      481 gaacggggaa gatcattccg ttttcctgca ttcttttcac gagcagcctg ttggattcga
      541 gcagcacacc cgtatcagat gatttgaccg acaatacaag cctggtaaaa ttctcggacc
      601 ggaaaatgtt tatgaattca agggccgact gtaccattcc ttccggggta ttcccgtaac
      661 gggtcatgat gcggtcggac agcgagccgt gattgattcc gacacgtatt gccgtatcat
      721 tctcccggca tatgtttatc aggggaagaa tcctctcatg gatcctttca agttcctcat
      781 tgtattcctt ttcactgagt tcattcctga tgaaggaagc cctcctgtcg gcatagttac
      841 ccggattgat ccttaccttt tcaactatcc ttgcagctgt ttcggcagcg gcgggattga
      901 aatgaatatc tgctatgaga ggagtgctga agcctgcttt cctgacctcc cttttgatgt
      961 tttcaaggtt gcgggcttcc tttatcccct gtgccgccag cctgaccatg tcggcaccgg
     1021 cttcgattat ccgaatgcac tgggcgactg atgactttgt atcgagggta tcggtattgg
     1081 tcatcgactg gatgcggacg ggataatgac ctcccagtgg cagccggccg atgaggacgg
     1141 aagcggattt ccggggagtt tccctgttat ttccggcatt tgtcattccc ggctccatgt
     1201 catcccctcc ctggtatcat tgagcacaat accggccttc ttcagctctt ccctgatatg
     1261 gtctgaaact tcccattcct tcctgttcct cgcatcctgg cgcagatcaa tgattatttt
     1321 catcagatcg gatgtgagct tttcatcatc cctgtctgcc gattcatttt tcagtcccag
     1381 tatgtcgaag acgaaagtgc tgaaaaggga tctgaaggtt tccaggtcgc tgccgctgat
     1441 cctgtccgtt ccctcattca cggagttgat atatcttatg ctgtcaaaaa gacgtgatag
     1501 aagcagaggg gtattcagat catcattcat ggcttcatag tatcccctgc cgactgaggc
     1561 aatatccaag gacgagtcct ttgagggctt caggttcctg agggccgcag ccgccttcat
     1621 gagtctgcgc aatcccttcc cggcagcgga gagagcttca tttgagaaat caacagtgct
     1681 gcggtaatgt gcctggagaa tgaaaaacct gatggtcatc ggactgtatg cctgctgaag
     1741 cagcggatga tcgcccgaaa agagctggtc gagtgtgatg aagttgccca gcgacctggc
     1801 cattttctga ccgttaatgg taataaggtt gttatgcatc cagtacctga ctgattcctt
     1861 tccgagggca gcggttgact gggctatttc gcattcatga tgaggaaact gcaggtcaat
     1921 gcctccgcca tgaatgtcaa agacctcccc gagatatttt gtgctcatgg ccgagcattc
     1981 caggtgccag cccgggaaac cggtgctcca cggagccggc catcgcatga tatgcccggg
     2041 agcggctttc ttccacaggg caaaatccca gggactgcgc ttttcactgc ttccttcaag
     2101 tgtgcgggtg ttttgctgaa gttcatccag cacccttccg gaaagcttac cgtaactgaa
     2161 tttctcatta tacttcggaa cgtcaaagta taccgaacca tttgcttcat aggcataacc
     2221 cgcgtcaatg atctttttga tcatttccac ctgctcaatg atatgtccgg atgcccttgg
     2281 ctcgatggag ggaggcaggg tgttgagctc ttccatgttt ttcaggaaat tgttcatgta
     2341 cttctgaaca agctccattg gttcaagatg ttccagacgt gctttcttcc cgatcttatc
     2401 ctcgccgtca tcggaatcat cctccaggtg tcccacgtca gtgatattcc tgacatagcg
     2461 gaccttatag cccagatgag tgaggtacct gaaaagcagg tcgaaggtaa ttgccgggcg
     2521 ggcatggccc agatgggcat cgctgtaaac cgtcggtccg catacgtata agccggcaaa
     2581 cggcggatga agcggctcaa acagttccat ttcccttgtg aggctgttgt atatataaag
     2641 attattctgc atgtaccatg aattagacca gtgcaaaaat acgagaaaaa tacttcaact
     2701 cacaggaaaa tgacccggct gtgccggaag ctcaatctta caggaacttc ggatcctgaa
     2761 aagacttttt gtaaaaaatc attcttatat ttgaccgcga aaaaatcgtt tggtccggga
     2821 aacacggatg ctcttcaaac taaaggtaat aatcaaccga taactgttat gccttctgtt
     2881 tttacctggt ttaaggaagc ggaaatgatt taagtaaata acatgaaata ttctcctgaa
     2941 gatataatta cacggaaaat cctcattctt gacggggcaa tgggaaccat gatccagaaa
     3001 cagggtctga atgaggattc ctacagggga tccctgttcg gggatcatcc cgtgccgctg
     3061 aagggaaact atgatatact gaatattacc agtcctgatg taatcaggaa gatccacctt
     3121 gattaccttg aggcgggagc cgatataatc agtacgaaca ccttcaatgc aaatgctgtt
     3181 tcacaggctg attaccggat ggaggaccag gtgtacagga tgaaccttga ggccgcacgc
     3241 attgccgggg aagccatttc cctgcatgag aaatccggcg gttccggcat tcattttatt
     3301 gccggggcaa taggacccac caaccgtacg gcttcaatgt cgtcagacgt aaacgatccg
     3361 ggtgcgcgcc agatcacctt tgatgaactg gctgaggcat attcggaaca ggtgagggga
     3421 cttattgacg gaggggctga tatccttctc atcgagacag tctttgatgt actgaactgt
     3481 aaggcggccc tgtacggaat aagcaatatt tttgaagaga ggggaataag tctgccggta
     3541 atggtttcgg gaacaatcac ggatgccagc gggcggacac tgaccggcca gacccttgag
     3601 gccttcctcg tttcggtctc ccattttcct ttgttcagca tcggactgaa ctgcgccctt
     3661 ggcgcggagc agctgagacc ctttgttcag gagttgtcgg ggaaaacaga attttatgtg
     3721 tctgcccatc cgaatgccgg actgccggat cagttcgggg aatacaatca gtcggccgga
     3781 tacatggcaa tggttaccga ggaattcatg aaggagggct gggtgaatat catcggcgga
     3841 tgctgcggaa caactcccct tcatataagg aagatcgcgg aactggcgga aaaatacccg
     3901 ccaaggcaga agcctgcgat aaagaaatac acgaggctca gcgggctgga agtactgacc
     3961 atcacgcctg aaaccaattt tgtaaatata ggtgaacgta ccaatgtgtc gggatcatcg
     4021 aaatttgcca ggctgatacg tgaggagaag tacagtgagg ctcttgccgt ggcaagaaac
     4081 caggtggagg gcggagccca gataattgat atctgcatgg atgaagccat gctggactcc
     4141 gaaagggcaa tggtaaggtt cgtgaacctg atcatggctg aacctgacat agccagactg
     4201 ccgctgatga tagactcctc gaaatggagt gtgatcactt ccgcactgaa atgcatccag
     4261 ggcaagtcgg ttgtcaattc catcagtctg aaggaaggag aggaagtgtt tctcggacac
     4321 gcacgggaaa taaggaaatt cggcgcggct gccgtcgtca tgcttttcga cgaaaaggga
     4381 caggccgaca gctatgagag gaagacagag gtcgcggaga gatcatacag gctgctggtt
     4441 gaaaaagcag gatttcctcc ggaggacata ttttttgacc ccaatgtgct cgcggtggca
     4501 acaggtatcg aggagcacaa cagttacgct gtcagcttca tcagggcaac cacctggata
     4561 aaggaaaacc ttccccatgc cagagtgagc gggggaatca gcaatctctc attctcattc
     4621 aggggaacgg acagggtaag ggaggccatg cattcggtat tcctttacca tgccatcaat
     4681 gccggcctcg atatggcaat agtgaatccg ggaatgttgc aggtatacag tgaaatagat
     4741 ccggatctgc tcaagctgac agaggatgtc attctgaacc gcaggaagga tgccacggaa
     4801 agactggtaa agtttgcgga aggactgaag aatgagggga agaaggagga aaagagcgat
     4861 gaatggaggg ccctccctgt tattgaaagg atcaggcatt cacttgtaag aggcctggat
     4921 gagcatattg agcaggatgt ggaggaggcg agaccccttt ttgccagatc catagaactg
     4981 attgaaggtc cgctgatgtc gggaatgaat gaggtgggag acctgttcgg atcgggaaag
     5041 atgttcctcc cgcaggtggt caagagcgcc agggtcatga aaagggcagt gtcaagactt
     5101 gaaccctata ttgagataga aagcaggcat gaggagaaga aaagtgccgg gaagattgtt
     5161 ctcgcaacgg taaagggaga tgttcatgat atagggaaaa atatcgttgg cgttgtattg
     5221 tcatgcaaca gttaccaggt gacggatctg ggagtgatgg cctccacgga aaaaatcatt
     5281 gacaccgccc tggaggagaa agctgatttt atcggactga gcggactgat aacaccttcg
     5341 ctagaggaaa tggtacatgt ggctgcggag atggaaaggc ggaagctgac aataccactc
     5401 cttatcgggg gtgccaccac ttctgaggta cataccgcgg taaaagtttc accaaaatat
     5461 tcagcacctg tcattcatgt aaaggatgca tcaaaggccg ccggggttct ttccgcactg
     5521 ctcagggaag acagggcaca atatgctgcc gctgtaagtg agagatacgc ttccctgagg
     5581 gaggaacata cagtgaagcg cagcgagagg aagctccttc cactagcgga tgccagggag
     5641 aaccggctga ataccgactg gaatacccaa aagcccttca ttcccctgaa gccgggatac
     5701 aggataattg atgacattga ccttaatctg ctgaccgggt attttgactg gacgttcttt
     5761 ttcgtgcagt ggaaactgac gggaagatac cctgccatcc tgaatgatcc ggtcaagggt
     5821 ccggaagcca gaaaactgta tgatgatgca caacaattac tgaaagaaat aattgaaagg
     5881 aaactgatat ccgcaaaggc cgttttcgga cttttcccag caggatcaga gggtgatgac
     5941 gttgctgtgc tcagcgaccg gaagggcagc aacaggacga cctggctgag gtttctcagg
     6001 aaccagggag tgaaggaaac agattttccg aacctgtgcc tggcggattt cattgcgccc
     6061 ctttcatcgg gagtggctga caacataggg ctctttgttg ttactgccgg tattgacgaa
     6121 gataaatttt ccatgtacag ggaggatgac tatgcttcga taatgatccg gatgctgagt
     6181 gaccgttttg ctgaggcagc cgccgaatgg ctccatgaaa gaatcagaaa ggaatactgg
     6241 ggctttgccg ccgatgagaa cctgcctgtt gaggatctgc tggcggtgaa gtaccgtggt
     6301 gtcagacccg ctccgggata tccggcctgt ccggatcata ctgaaaaaag ggtgctgttt
     6361 gacctgctcg atgctgaaaa tgcaatcgga gccggactta cggaaaattt tgcgatgatc
     6421 cccccgtcag ccgtgagcgg atatatattt tcacatccgg cttccacata tttcactgtg
     6481 ggaaggatag gaaacgacca gttaaaggat tatgctgaaa gaaagaatat gagtattgat
     6541 gaggcagccc gctggcttgc ccctaattta tgacaagata tatttatgag tgttattgaa
     6601 aaaataaaaa atgccaaggg gcctctgttc acgtttgaac tgcttcctcc cctgaaaggg
     6661 cacagcattg agcgtatcta ttctgcgatt gacagactga tggagtttga gccggcctac
     6721 attaatttca cttcgcaccg gaatgagacc agttacaaag aaaggcccga cggacttctt
     6781 gaaaagaggg tgacgcggct ccggcccgga acaatcgcac tggctgctgc cgtgaaatac
     6841 aagtacaaca tcacggtggt gccccatatc ctctgcgggg gcttcaccag ggaggaaacg
     6901 gaaaatgtgc tgatagaaat gaatttcctc aatattgatg atgtcctggc cctccgggga
     6961 gaccctcaga gaggaagccg tgtctttatc cccgagaagg acgggcacag gtataccagc
     7021 gagctggtgg cgcagattgt ggatatgaac aatggaaggt acctggagga tacactcagg
     7081 gaaaatactc ccaccagttt ctgcatcgga gtggcaggat atcctgaaaa gcacggtgag
     7141 gcaccgaata tggaaaccga tattgtaaat ctaaaaaaga aaattgatgc cggagctcag
     7201 tatattgtta cccagatgtt ttttgacaat ggaaaatact tccggttcag ggatctgtgc
     7261 agggatgcag ggattcatgt cccggtgata ccgggaataa agcccgtttc cgccctgaat
     7321 gacataaaaa tgcttcccaa gacctttcat attgacctgc cgaatgacct ggtgaaagaa
     7381 gtggaaaaat gcagcaccga tcaggaagcc cgtgaagtgg gaattgaatg gaccactatg
     7441 cagtcaaggg aactgatcag ccggggagcc cccggaatac attattatac cctgggccgt
     7501 tcagataata ttgccaggat agttaaggct tcgttctaga ttccggatcc gtgatccgta
     7561 ccgcttccgg atctgtaccg taccggatta tctgattcgt attgcctgtt gcgggtcaga
     7621 taaatcaggc agtactggct ggttaacgtc ctgtaaggaa gttatttcag ataccgaatt
     7681 cgttccggat cttatccaca tagtcgagtt tttcccatgc gaaaaactcc acttccttca
     7741 tccatccctt tttgccgttg ttgttgagga tccgcggtat gcttccggga acctcatagt
     7801 acacctcaac attgccgata tgtggttcct ttccgaaatg gccgtatgaa gcggttttca
     7861 ggaatacggg attcttcagt cccaggcgtt cgataatgaa atagggtctc agatcgaaaa
     7921 gtttcctgac cctggaagca atttccccgt cggtaagtat ccttccgctg ctgtctttta
     7981 ccctggccgt tccgaaggtg ttcacaaaaa agcctaccgg ttgggcaatc ccgatagcat
     8041 aggcaacctg tatgagtgcc tggctgcata cgcccgcagc taccatgttc tttgctatat
     8101 gacgggcggc ataggctgcc gacctgtcaa ccttggaagg atccttgccg gaaaatgctc
     8161 ctccgccatg tgcaccgtga ccgccataag tgtctgtaat gatctttctt ccggtcagtc
     8221 ccgtgtctcc gtgggggcct ccgattacaa acttgcccgt gggattgacc aggagccgta
     8281 catcatcacc caacagcatt tttacgtttc cgggaaggga ttcccttact cccgggaaaa
     8341 gatatttctt caggtcagcg gcaatc
//
LOCUS       DAOSPX010000121         8365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8065_length_8365_cov_3.774248, whole genome shotgun sequence.
ACCESSION   DAOSPX010000121 DAOSPX010000000
VERSION     DAOSPX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8065_length_8365_cov_3.774248"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..650)
                     /locus_tag="PLZ75_07290"
     CDS             complement(111..650)
                     /locus_tag="PLZ75_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOS72234.1"
                     /translation="MLSKRLKKYFFCLAATLIVTGTWVKAQTGTTASATGHIIAEVIT
                     VYSATEMSQMNFGRFYPGPHGGEIILTPESTVSVMGSVYKEAGTQTAASFYVSGHAGT
                     SFTITLPREPVVLNHVSSARTMSISDWVSNPGIEPAAGILQNGSQMVYVGATLKIGTL
                     EDNPVGIYAGTFNITFDFN"
     gene            complement(671..1198)
                     /locus_tag="PLZ75_07295"
     CDS             complement(671..1198)
                     /locus_tag="PLZ75_07295"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOS72235.1"
                     /translation="MKKLFALSVILIAFTAGAFAQTNQATATATATIVTPITIEATGN
                     LSFGNIVPHASTSGSVTISPDGSASYSTPDIQYSASPTATAARTAAIFTVRGTPGASY
                     SITLPSGNVELNGPNSNTMNAHTFTSDPSINSGQLSNPGGTQTLRVGATLNVTGGQAP
                     GTYTSDNFTVTVNYN"
     gene            complement(1474..3840)
                     /locus_tag="PLZ75_07300"
     CDS             complement(1474..3840)
                     /locus_tag="PLZ75_07300"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOS72236.1"
                     /translation="MKNSLLLKICVLLFPVFTLSSCDQPDKNPSSFVNPFTGTQYFGH
                     TYPGAALPFAMVHVSPDCNNTGWTYAAGYNWQDSSIMGFSHTHFSGVGMTSGGEILIQ
                     PAVNRKLHIVPGPAENPDEGYRSRFDHRDETASPGYYSVLLKDYGIKVELTAGKRTAF
                     HRYTFPETENAHIIIDLGHQIGTARAPEMSEISVSGNTRIEGYKSTPAGRVYFCAEFS
                     KPFLYYGTFDADRKTPESDSGIFPYKSGEKGERIGAFVNYKTSEKEQILVKVALSYTG
                     PEGARKNLEAELADRDFDRVKKDAGDIWNRELSRIEIDGATDEQKEIFYTSLYHSLLA
                     QYTGEDADGKYYGMDGRVHTAEGYDFYPSFSCWDTYRSQHPLLTIIAPEHVNSFLKSI
                     EAKIRDFGWLPAQHFSNVYRESMVGDHLIPIVTDAYVKGYRDFDAEFVYEAMKKKALE
                     KPEPPVPENAGRSGLGYYISLGYTPIDKVTEAVPNTMELAYDDWCTGVMAEALGRQKD
                     MELFMKRALNYRNVWDSTSGFMRPRKADGSFLEELNGREQTIVTKNGHSYYKYFDPLL
                     VGVRPNRHYTESNAWQYIWSVQHDIKGLIDLFGSKEKFNDKLDTFFDMDPSVTGPKYI
                     GVVGTIGQYVHGNQPSHHVAYLYDYSGEPWKTQQRTRQICDQLYRPGPGGLCGNEDMG
                     SLSSWYVFSSLGFYPVTPGSTSYAIGSPVFGKAVIHHPDGKKFTVRAINNSDKNIYIQ
                     SATLNGIPLEKPFIEHRDIMNGGELLFIMGPEPSRNWGISAPPPSLSD"
     gene            complement(3852..4778)
                     /locus_tag="PLZ75_07305"
     CDS             complement(3852..4778)
                     /locus_tag="PLZ75_07305"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOS72237.1"
                     /translation="MKQKIMPLAALAFFIFCNISAAQSFTLNLWPDGIPGSLISAEYK
                     EVSVVEDGIITRWNKVTDPSVTVCLPPEDKATGTAVLICPGGGYGTLAFNHEGNDIAK
                     WLNDNGIAGIILKYRLPSDQIMKDKSNGPLQDAQEALRIIRRNARAWNINPSRIGVIG
                     FSAGGHLASTLSTHFDEKVYEPKDNTSARPDFSILIYPVISMDESFTHMGSRRNLLGE
                     NPAAETLRHFSNELQIRENTPPAFLVHSADDAAVPVRNSMAYYEGLVKFNIPSELHVF
                     QKGGHGYGLAVNRETQSAWPRLCLEWLKASGF"
     gene            complement(4845..5699)
                     /locus_tag="PLZ75_07310"
     CDS             complement(4845..5699)
                     /locus_tag="PLZ75_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72238.1"
                     /translation="MRNKLYILLFNLPLLFTLNAQDIHQIRQFGDEQFRNGDYRLALK
                     EYQRVLLFDNERVHNDLYSKIAFIQYYSNEYEQAMRYFDYAIITERNDSLRFELMLKK
                     ALCCFRMNNYLAALNELYDLPDPPGRFLQNKKNLYMGICCFGMNDYENSKGCFSALLD
                     SLGIRNLEEVFNDFSRFNKKFRPGKAELMSMILPGLGQIYTGNVTDGLNSFLLISGVT
                     IYSLVTAVNYAIIDGLLVLSSWFYRYYSGGFSNASDLAQKKLDRKKEEVYAEILALIE
                     SNQLNLQE"
     gene            complement(5668..6510)
                     /locus_tag="PLZ75_07315"
     CDS             complement(5668..6510)
                     /locus_tag="PLZ75_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72239.1"
                     /translation="MIRRCRIILPAFIIAFTSIFPAVSQQNLLDYENSLKFAGYLKST
                     LQYDFAMQEYERINFLWPGDSLVIMELVQTYRLNDQCDRLDRAFTLIQDNISKHNSVR
                     SAKEYLNFALKCKSESSRFFDISIKLKPEERTYYNLAYYWVNQNYNSAITYCRTRIES
                     GLAAKSDLFQLTVDFQNEKYKSPAVSVLMSAVVPGSGKLYSGRFGDAMVSFLSVTTNT
                     WAAWRAFNKKGIRSANGWIFGSLAFGFYSANLWGSAKAAKTYNSNLKKRYQSNAEQII
                     YSSF"
     gene            complement(6494..6706)
                     /gene="yidD"
                     /locus_tag="PLZ75_07320"
     CDS             complement(6494..6706)
                     /gene="yidD"
                     /locus_tag="PLZ75_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOS72240.1"
                     /translation="MYKKLISSQDIPKCVFTPSCSEYAIEAIRKRGLFTGLLAAFDRL
                     TRCHGLVKPGHYPFDTQKNRFYDPPL"
     assembly_gap    6708..6717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6919..7680)
                     /locus_tag="PLZ75_07325"
     CDS             complement(6919..7680)
                     /locus_tag="PLZ75_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72241.1"
                     /translation="MSRSEDTSIEVSSLYILKIKLKTMLDPARAKCWLLILISTILFS
                     ISGYGQDYFRIRADFSVKISNSNGTKNLTRGIVYYDKNIRDLIYDISFPRKEKWISRD
                     TCLYKFSNDSLIQKVTIPSINEFTIFHLALNSRLNDFGLKNTMYQMNKVEKKGGMVLS
                     YWKIPEKAKISIDHVVVAKKSNRLESVVMVNSDQKIISKQFFRNYIKIDAFEFPSQII
                     QILYDPDGRENYQVTEFRNIRINDMTNSKLYESAL"
     gene            complement(7722..8219)
                     /locus_tag="PLZ75_07330"
     CDS             complement(7722..8219)
                     /locus_tag="PLZ75_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72242.1"
                     /translation="MKKIMLKSLSVFLTLVFLTAQSFSLSAGTIMSPSHSLDESVFGY
                     DQNALESAFFELDELDNYLEQNEGLTYNDLQAAGNVLIAGISDISAPLGLPDGDDELP
                     LGIPAFWWGCILGWVGLLVVYLVTDKDKEQTKKALTGCLISTGVSVAISVVYWVWIFG
                     ATKDL"
BASE COUNT         2235 a         1805 c         1884 g         2431 t
ORIGIN      
        1 aaatcccaga atgagagtca gaaaaaaagc aagcgaggct gacctgatta caggcagcct
       61 cctgagagga attaatatgg tcggtagcat ctggatttat ttttagggcg ctaattaaaa
      121 tcaaaggtta tattaaaggt tcctgcatag ataccaactg gattatcttc cagtgtgcct
      181 attttaagag ttgctcccac atagaccatc tgagacccgt tttgcagaat tcctgcagca
      241 ggttcgattc ccggatttga tacccagtcg cttattgaca ttgtccttgc acttgaaaca
      301 tgattgagaa caacgggttc ccggggcagt gtgatagtaa aggaagttcc ggcatgtcct
      361 gaaacataaa aacttgctgc agtctgggtg ccggcctcct tataaacgct tcccatgact
      421 gatacagtgc tttcaggggt tagtatgatc tctcccccgt ggggacccgg gtaaaatctt
      481 ccgaaattca tctgcgacat ttcagttgct gaatatacag tgatgacctc agcaattata
      541 tgtccggtgg cacttgccgt agttccggtc tgtgccttta cccaggttcc tgttacaatc
      601 aacgttgctg caaggcagaa aaaatatttt ttaagccttt tgctgagcat ggctgataac
      661 tccttaattt ttaattataa ttgacagtta cagtaaaatt atctgaagta tacgttccgg
      721 gtgcctggcc cccggtaaca ttgagtgtgg ccccgacccg taaggtttga gtgccgcccg
      781 gattactgag ttggccacta tttatactgg gatcacttgt aaaagtatgg gcattcattg
      841 tatttgaatt cgggccgttc aactcgacgt ttccacttgg aagtgtaata ctataagacg
      901 cacccggagt acctcttaca gtaaatatcg ctgcagtcct ggcagctgta gcggtgggtg
      961 aagcagaata ttgtatatct ggtgtggaat atgacgcaga gccatccggg gaaatagtaa
     1021 cagatcctga tgtggaagca tgtggtacaa tattgccaaa gcttaaattt ccggttgctt
     1081 caattgttat aggtgttaca atggttgccg tggctgtagc tgtagcttga ttggtctgtg
     1141 cgaatgcgcc ggcagtgaat gcgatcagaa taactgataa tgcgaagagt tttttcatca
     1201 ttttgaattt aataagacaa cagacttttg ttttgcaaac ttattttatt ggtcttaaac
     1261 gttgtttaag cctctttgtt tcacctcaaa ataaagctat cacatcaaaa cagcagaatt
     1321 gtagcactaa aataaatgaa attatcttca gcccgaagtg tttttgatta acattattta
     1381 acaacagcct gaagttttca acatactgat gtacagaaag ctaacttttc aggagaaaat
     1441 tgcccgaaat gagttttgta ataatccggg aaatcagtct gagagggagg gcggaggtgc
     1501 agatattccc cagttcctgg agggttccgg tcccatgatg aagagcagtt cgcctccgtt
     1561 cattatatcc cggtgctcaa tgaatggttt ttcaagaggg ataccgttaa gggttgccga
     1621 ttgaatataa atatttttgt cagagttgtt tatcgccctg acagtaaatt ttttaccatc
     1681 cggatgatga atgactgcct ttccgaaaac cggactgcct atggcatagg aagtgctccc
     1741 gggagtcaca gggtagaatc ccagtgaact gaaaacatac catgaggaga gtgagcccat
     1801 atcctcattt ccgcacaggc cgcccggacc gggacgatac agctgatcac aaatctgcct
     1861 ggtcctctgt tgtgtcttcc agggctcgcc tgaatagtca tacaggtatg caacatgatg
     1921 cgagggctgg tttccatgca catactggcc gatggtcccg acaacgccga tatatttagg
     1981 acctgttact gaggggtcca tatcaaaaaa tgtgtcaagc ttatcgttga atttttcttt
     2041 gcttccgaaa agatctatca ggccttttat gtcatgctga acggaccaga tgtactgcca
     2101 tgcattcgat tcggtgtagt gcctgtttgg ccttacccct acaagaagag gatcgaaata
     2161 cttatagtag gagtgtccgt tttttgtcac gatggtctgt tcacggccgt tcagctcttc
     2221 caggaatgat ccgtctgctt tacgcggcct catgaatccg cttgtgctgt cccatacatt
     2281 cctgtagttc agggcccttt tcatgaacag ctccatgtcc ttttgccttc ccagtgcttc
     2341 tgccatcact cctgtgcacc agtcgtcgta tgccagttcc atggtgttgg gaacagcttc
     2401 cgtgacttta tcgatgggtg tgtaacccag acttatataa tatccgagcc cggatctgcc
     2461 ggcgttttcc ggaacaggag gctccggctt ttccagggct ttcttcttca ttgcctcata
     2521 aacgaattcc gcgtcaaaat ccctgtatcc cttaacgtag gcatcagtaa ctatgggtat
     2581 gaggtgatca cccaccatag actcgcggta gacatttgag aaatgctgtg cgggaagcca
     2641 tccgaaatcc cttatcttgg cttctatgga tttgagaaaa ctattcacat gctcgggcgc
     2701 aatgattgtc agaaggggat gctgcgacct gtaggtgtcc cagcatgaaa atgaagggta
     2761 gaaatcatat ccctcggcag tatgcaccct gccatccata ccgtaatatt ttccgtctgc
     2821 atcctcaccg gtatactgtg ccaggaggga atggtagagg gatgtataaa atatttcctt
     2881 ctgttcatca gtagcgccat ctatctctat acgtgagagt tccctgttcc agatatcccc
     2941 ggcatccttt ttcacacggt caaaatcccg gtctgccagt tcggcctcaa ggtttttacg
     3001 ggctccttcc ggaccggtat atgaaagagc cacttttacc aggatctgtt ctttttcaga
     3061 tgttttgtaa ttgacgaaag cgcctatcct ttctcctttt tcaccgcttt tgtagggaaa
     3121 gattccgctg tcagattcag gagtcttcct gtctgcatcg aaggttccgt agtacaggaa
     3181 aggcttgctg aattcggcac agaaatacac tcttccggcg ggggtgcttt tatatccttc
     3241 aatgcgtgtg ttgccggaaa cgctgatttc agacatttcc ggtgcccgtg cagtgcctat
     3301 ctggtgaccg agatcaataa tgatatgtgc attttctgtt tccgggaagg tatagcggtg
     3361 aaaagccgtc cttttgccgg cggtaagttc aacctttatg ccataatcct tcaacaggac
     3421 ggaataatac ccgggtgaag cggtttcatc cctgtgatca aagcgggaac ggtagccttc
     3481 atcaggattc tccgccggac cgggcacaat atgcagcttc cggttaactg caggctggat
     3541 aagtatctct cctccgcttg tcattcccac accgctgaaa tgggtatggc tgaatcccat
     3601 tatggaactg tcctgccagt tgtagccggc cgcgtaagtc cagccggtat tgttgcagtc
     3661 ggggctcacg tggaccattg caaaggggag ggctgccccg gggtaggtat ggccgaaata
     3721 ttgtgtgccg gtaaaggggt ttacaaagga ggacggattt ttgtccggct ggtcacatga
     3781 acttaatgtg aatacaggaa acaacagtac acatattttc agcagaaggg aatttttcat
     3841 gatccgggct tttaaaatcc tgatgctttc agccattcca ggcacaggcg gggccaggcc
     3901 gactgtgttt ccctgttgac agccagaccg tatccgtgtc ctcctttctg gaagacatga
     3961 agttcggaag ggatattgaa tttcacaagt ccttcgtaat acgccatgct gtttctgacg
     4021 ggtacggccg catcatccgc ggaatggaca aggaaagcgg gaggagtgtt ttccctgatc
     4081 tgcaactcat tggagaaatg gcggagagtt tctgcggcag ggttctcccc caggagattt
     4141 ctccgtgatc ccatgtgtgt gaatgattca tccatactta tcacaggata gataaggatg
     4201 gagaaatcag gacgtgcact tgtattgtct tttggttcat aaactttttc atcgaaatgg
     4261 gttgaaaggg ttgatgcaag gtgaccccct gcggaaaagc ctattacacc gatacgggag
     4321 ggattaatgt tccatgccct tgcattccgg cggatgatac gaagggcctc ctgggcatcc
     4381 tgaagaggac cgttggattt atccttcatg atctgatcgg agggaagcct gtatttaaga
     4441 attattcctg caataccgtt atcgttcagc cattttgcaa tatcgtttcc ttcatgattg
     4501 aatgccaggg tcccgtatcc tccacccgga caaataagta ctgccgtgcc cgttgccttg
     4561 tcctccgggg gcaggcaaac ggtaacggaa ggatcggtga ccttgttcca tctggtgata
     4621 ataccgtctt caacaacgga gacctccttg tattccgcag aaataaggga accaggtatg
     4681 ccgtcaggcc ataagttcag ggtaaaagac tgtgctgctg agatgttgca gaagatgaaa
     4741 aaagcaagag cggcaagggg cataattttt tgtttcatga ctaatctatt ttaattccta
     4801 aaataagggg aaggctgcga tatgtcctga aaaccagaag caggtcattc ctgtaaattt
     4861 aactgatttg attcaatcag ggcaagaatt tcggcatata cttcttcttt ttttctgtcg
     4921 agtttttttt gtgccaggtc agaggcattg ctgaaccccc cggaataata ccggtaaaac
     4981 caggaagaaa ggaccagcag accatcgatt atggcatagt ttacagctgt aaccagggaa
     5041 taaattgtta caccgcttat caggagaaat gaattgagcc cgtcggtaac gttaccggta
     5101 tatatttgtc cgaggcccgg caaaatcata ctcatcagtt ctgctttccc cggcctgaat
     5161 tttttattga aacggctgaa gtcattgaaa acttcttcta aattgcggat cccgagcgaa
     5221 tcgagaagag cggaaaaaca gccctttgaa ttttcataat cgttcatgcc aaaacagcat
     5281 attcccatat acagattctt tttgttctga agaaatctgc cgggaggatc cggaagatcg
     5341 tacagctcgt tcaacgctgc caggtaatta ttcattctga aacagcataa agcttttttt
     5401 agcattaatt caaacctgag actgtcattc ctttcggtta ttatggcgta atcgaaatac
     5461 ctcatcgcct gttcgtattc attggaataa tattgtatga aggcaatttt cgaatataaa
     5521 tcgttgtgga ccctttcatt gtcaaacaaa aggacgcgct ggtattcttt aagtgcaagc
     5581 ctgtagtcac catttctgaa ttgttcgtca ccaaactgcc tgatctgatg aatatcctgg
     5641 gcattcagtg tgaaaagcag gggcaggtta aaaagaagaa tatataattt gttccgcatt
     5701 ggactgatac ctttttttca ggtttgaatt atatgttttg gcagcctttg ccgaacccca
     5761 caaattggcg gaatagaaac cgaaggcaag actgccgaag atccagccgt ttgcgcttcg
     5821 gattcctttt ttgttaaagg ctctccatgc agcccatgtg tttgttgtga cagaaagaaa
     5881 cgaaaccatg gcatcgccaa aacgccccga atagagcttg ccgcttccgg gaacaactgc
     5941 tgacattaat accgaaacag caggactttt atatttttca ttctgaaaat caacggtaag
     6001 ctgaaacaga tcggattttg ctgccagtcc tgattcaatc cgggtccggc aatatgtgat
     6061 agctgagttg taattctggt ttacccagta gtaggcaagg ttataataag ttctttcttc
     6121 aggtttcagt tttatcgata tgtcaaaaaa ccgggatgac tctgacttgc atttaagggc
     6181 aaaattaaga tactcttttg cagaacggac agaattatgt ttactgatat tatcctgtat
     6241 aagtgtaaat gccctgtcaa gcctgtcgca ctggtcattc agcctgtaag tctgtacaag
     6301 ttccataata accagtgaat caccgggcca caggaaattt attctttcat attcctgcat
     6361 ggcgaaatca tactggaggg ttgatttcag gtagccggcg aatttcaggg aattttcata
     6421 gtcaagcagg ttttgctgtg aaacagccgg aaaaattgat gtgaaggcaa taatgaaagc
     6481 agggagaatt attctacaac ggcggatcat aaaaccggtt tttttgagta tcgaaggggt
     6541 aatgacccgg ttttaccagg ccatggcagc gtgtcaggcg gtcaaaagca gccagcagac
     6601 ctgtgaataa tcctcttttt ctgattgctt cgattgcata ttccgaacag gatggtgtaa
     6661 aaacgcattt cggtatatcc tgtgaggaaa tcaatttttt atacagtnnn nnnnnnnaaa
     6721 ggcagttgcc gcagttattt gaatttcatt tgttgcttcc ttcagaggcc cggtaaaatc
     6781 ccggcggagc gtgtccgggg aactgaacaa tgatttcagc tcaggatcac atagagattg
     6841 cgcggaaata gcggaaacaa agaaaatgtt gaaaagaaaa ataaagatta tgaatgattt
     6901 ccggttattc atttcaattt ataatgcaga ttcgtataat ttgctgtttg tcatatcgtt
     6961 tattctgata ttcctgaatt ccgtcacctg gtaattttcc ctgccatcag gatcatacag
     7021 aatctggatt atttgtgagg gaaactcaaa agcatcaatt ttaatataat tcctgaaaaa
     7081 ttgtttgctg ataatcttct gatctgagtt taccataaca acactttcaa gcctgtttga
     7141 cttttttgcc acaaccacat gatcgattga tatttttgcc ttttccggaa tcttccagta
     7201 tgacagtacc atgcctccct tcttttcaac cttgttcatc tggtacatcg tgtttttcaa
     7261 tccgaaatca ttcagtctgg agttaagggc cagatggaaa atggtgaatt cgttaatgga
     7321 tggaatcgtc actttctgga ttaatgaatc attgctgaac ttatagagac aggtatccct
     7381 gctgatccat ttttccttcc tgggaaatga gatatcatat ataagatccc tgatattctt
     7441 atcatagtaa actattcccc tggtcagatt ttttgttccg ttgctgttac tgatcttaac
     7501 ggaaaagtca gccctgatcc tgaaataatc ctgtccgtaa cctgatatac tgaacaatat
     7561 tgtactgatt aaaatcagca accagcattt tgccctggcg ggatcaagca ttgttttcag
     7621 ttttattttt aatatataaa gcgaagatac ctcaattgag gtatcttctg atctgctcat
     7681 gaaacagatt gtaacagatt gatgtatagt attacttaaa attaaagatc cttcgtggcg
     7741 ccaaagatcc atacccaata aaccacgctg atcgcaacac tcaccccggt ggaaatcaga
     7801 cagcctgtaa gagctttttt ggtctgctcc ttgtccttat cagtaacaag atagactacc
     7861 agcaagccaa cccatcccag aatacatccc caccagaaag ccgggatccc caggggaagc
     7921 tcatcatcgc cgtccggcag gcccagaggg gcggaaatat cactgatacc tgcaatcagt
     7981 acattgccgg cagcctgtag atcgttatag gtcaggcctt cattctgctc aagatagttg
     8041 tccagttcat ccagttcaaa aaaagctgat tcaagagcat tctgatcgta accgaaaaca
     8101 gattcatcta ggctgtgcga tggggacatg atggtaccgg ctgaaagact gaagctttga
     8161 gccgttaaaa acaccagtgt cagaaagaca cttaaactct taagcataat tttcttcatg
     8221 atatcaattt ttagaattct gatcagaggt tcataatatg tagaaaatcc tgtctgataa
     8281 taattaagta aaaatatgaa tatttcttta ttctaacctg aattattcat aagtacttaa
     8341 aaaaaagggt gcttttgttc tttaa
//
LOCUS       DAOSPX010000122         8344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8088_length_8344_cov_4.985282, whole genome shotgun sequence.
ACCESSION   DAOSPX010000122 DAOSPX010000000
VERSION     DAOSPX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8088_length_8344_cov_4.985282"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLZ75_07335"
     CDS             complement(<1..612)
                     /locus_tag="PLZ75_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS72243.1"
                     /translation="MTPARTFDYLDILRKKFPKDDIFARYSGGEWIKVSVDEYIEASW
                     NVASGLISAGYKAGEKVIVICNNRPEWNFLDMGCTLSRLIFVPVYSTLSESEFLHVFN
                     HSDARLVVAGNLSLYNKLAPIVARMDHRAEMMIIDGSGSEYSLDDLIDLGKRNRKVND
                     PMIEHNRAEVSPDDVATIIYTSGTTGESKGVMLTHRNLTFNANG"
     gene            685..1233
                     /locus_tag="PLZ75_07340"
     CDS             685..1233
                     /locus_tag="PLZ75_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72244.1"
                     /translation="MKGQARRRSATTNPNSGYANFNEPAAGYGLGTTSLPNPEYYYGG
                     TSTHGYRLNIYGLNINRSFFAGLGSGVLFSEGDLMIPFYLDLRYFWNSKTISPYIMGE
                     GGFLFNPRDVDKKTLLMIIGGGGIQFNISDKFILNAGPGPGIQMGIDGRRSFLSGRLG
                     LGFKLSSPHGGSGLNQIFSFRK"
     gene            complement(1205..1777)
                     /locus_tag="PLZ75_07345"
     CDS             complement(1205..1777)
                     /locus_tag="PLZ75_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOS72245.1"
                     /translation="MKSFARYKTIVALMLVLITQLLVSHCTRKIYPEPSFSNIESSAD
                     KQRLQIFLDSGTEKGLDTRRANPDGIVRAARKYLGVPHCMGGKSMKCMDCSGLLVAVF
                     AGYGINLPHNSEEQARYGKLITDRKELRKGDLVFFIRSYKTSHFITHSGICTGNNSFI
                     HTSSSRGVTITSLDDAWWKERFIFGTRIFD"
     gene            1947..3101
                     /locus_tag="PLZ75_07350"
     CDS             1947..3101
                     /locus_tag="PLZ75_07350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 domain-containing metallophosphoesterase
                     family protein"
                     /protein_id="HOS72246.1"
                     /translation="MKRFYSLSVLITFLSLYSFSQMIDNDNLVITHGPYLQNLDASGV
                     TIIWTTNKPAVPGISLTYPDGLKRFIRNSHDGIIDGGGLLHKVRVEGLEAGTTYQYSI
                     NSIQILKYQAYKIYYGDTIDMKPLSFTTLNPKAEKTNFTVVNDVHEYSGKLASYIRNG
                     NPAAQEFFIYNGDMVNYVQSPEQLFTGFIDTTVAYFARTRAFFYARGNHEARGYAGRE
                     FKNYFDFRDNRFYYSFDHGPVHFVVLDCGEDKPDDNRYYYGLADYDSYRLEQLEWLKK
                     EVKSDAFRKAKYRIVIVHMPILNEGSRGHGMKFLADNFGPVLKTAKINLMLSGHTHRN
                     SFHARGEAGQEYPVLVNSNNSFVEIYVDKEKIRAVVRDVNGGNIAEYEFR"
     gene            3247..5478
                     /locus_tag="PLZ75_07355"
     CDS             3247..5478
                     /locus_tag="PLZ75_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOS72247.1"
                     /translation="MKKKRKIIVISFLILLLFPLLFFIVVICLASRNLQTSEELRNFK
                     NAEASRVLSSGGQLLGKFYIENRMNVTWGQLPAHLVNALVATEDVRFFDHEGVDSRSL
                     YRVLFKTLLLNRSESGGGSTISQQLAKNMFGRKAGGSFSVPVNKYREIILARRLEKTF
                     TKEEIITLYLNTVSFGEDIFGIDAAAGRYFNKITESLRIEESAMLVGMLKATTRYNPA
                     LKPENALSRRNVVLRQMEKYNYLGSSDADSLTALPLRLDYRKAESEGRADYFLVRVRK
                     ELDEILGNIALSTGRRWCPEKDGLIITTSLDDDLQSFARQSFARHLSTMQKHLRDQYS
                     TAAGRAKLNELTEAILLEKNLGHRAGKINRQLIFDWKGSYPDSISVRDSIKLALTTLH
                     GGLLAMDPQSGAIRAWVGGIDFITQPYDQILARRQLGSVFKPVFYALAFEEGMHPCQY
                     LDNDSLVLRDYQDWRPENFDRSYGGKYSVAGALARSMNIPSFVLFRELEFEKLNLLWK
                     EMGFTFPLRNTPALALGTAEASLEEIAVAYSVFANGGFKIKPWSVTSVIEPDGNIIYL
                     RNRDEIFPRILSERSCHLINAVLQKAVREGTGTSMQSVYGVSSAFAGKTGTTQNYADA
                     WFAAYNPGLTVVARTGASLPSIHFNNRYHGTGSVLALPLVALTLKKAESRPVTAGRFI
                     KPFPPLPPELQGSLDCPDFIEDKLPFRQTDRIRKQRTDRNYGTDRRKSFFLERLFRRN
                     RRN"
     gene            complement(5494..6279)
                     /locus_tag="PLZ75_07360"
     CDS             complement(5494..6279)
                     /locus_tag="PLZ75_07360"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOS72248.1"
                     /translation="MAKIYYAGDWAVLTGPTFAESPFQFAPKGLEIFNYGKWLKEALE
                     SDGKHQVDSVPGWDFYKLGPGEYEKILEEYDVIIFSDIEGKLFQLAPHFFVREEFGKK
                     ILVYPDRIRLTAEAVAAGKGLMMLGGWYSFTGELGKGGWGRTEFRSILPVECLPHEDL
                     VESTEGFYPKLTDAGKKVFFESDFEGMPPILGYNETRVIPEGEVLVTIEETGHPLVAT
                     RKIGRGRVLAYTSDPAPHWGLNFVYWEHYNNFWLKCLSLVLPE"
     gene            6651..7139
                     /locus_tag="PLZ75_07365"
     CDS             6651..7139
                     /locus_tag="PLZ75_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS72249.1"
                     /translation="MKNRQRILSILFLTLFSISYLQGQQAVVTSGGIATGTTGSVSYT
                     VGQIAYQTFTGTGGTLQQGVQQPWEVSTPVAVAETEDISLLMNVYPNPTSGAFKLIVG
                     TLENRNLRFRLYDMNGLLLQDKKIDSEETEIFLQDLSSSMYFLKIINNASEIKVFKIV
                     KK"
     gene            7146..8213
                     /locus_tag="PLZ75_07370"
     CDS             7146..8213
                     /locus_tag="PLZ75_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72250.1"
                     /translation="MKKLFTLMLMVLLTITILAQAPSKMSYQFVIRNTSGSLVVNSPV
                     GVRISILQGSPAGALVYRELFNPNPQTNANGLVSIEIGTGLPITGTFSSINWAGGPYY
                     LLTETDPSGGTSYTVTGTSQLLSVPYSLYSAMSASVSDNSITSAKIANGTIIAADLAD
                     GSVTSAKIADGTIATSDIANNAVTGAKIADETITAADIQNRQRNIVFPANALNYDKGS
                     SILTQDAYGIYWKSNFADGAYLTIPRPLDWVGTSNVILKLHFMKGGATGGVVNFFIRP
                     RSYNPGDSYADAPAQFPDSPVTVAAGTDHGIYTQTFTIPYSKFGTKNLWVITIQRKGS
                     GETFTGDLILMAVEIVYMAEQ"
BASE COUNT         2369 a         1974 c         1856 g         2145 t
ORIGIN      
        1 accgttggca ttgaaggtaa gattcctgtg cgtaagcata acccctttcg attcaccggt
       61 ggttccggaa gtgtaaataa tcgttgccac atcatcaggt gaaacttcag ccctgttgtg
      121 ctcaatcatg ggatcattca ccttcctgtt cctcttcccg aggtcaatca ggtcgtcaag
      181 gctgtattct gatccggagc cgtcaattat catcatttca gcccgatgat ccattctggc
      241 tacaatgggg gcaagtttgt tgtacagcga caggtttcct gcaacaacga gcctggcgtc
      301 actgtgattg aaaacatgaa gaaattcact ttcgctcagg gtagaataca cgggaacaaa
      361 aatcagtcgc gataaagtgc atcccatatc cagaaagttc cattcaggac ggttgttgca
      421 aatcacaatt actttttctc ccgccttgta tcccgccgat ataagaccgc tggcaacatt
      481 ccatgatgct tcaatatatt catcaacact tacctttatc cactcgccac cggaatatct
      541 ggcgaaaata tcgtccttgg ggaatttttt cctgagaatg tcaagataat cgaaggttcg
      601 ggctggtgtc atagcagggt ttaattcaaa atgtaaaaat aaaaatattc ctgtcagttt
      661 taaattgtgc cgtgcgccgg cgccatgaag ggtcaggcaa ggagaagatc tgccaccaca
      721 aatccgaatt ccggttacgc gaatttcaat gagcctgccg caggatacgg tctgggcacc
      781 acatcacttc caaaccctga atattattat ggcggaacaa gtacacacgg ataccggctg
      841 aatatttacg gactcaatat caaccgcagt ttttttgccg gtctcggatc gggagtcctt
      901 ttctctgaag gagatctgat gattcccttc tatcttgacc tcaggtattt ctggaacagc
      961 aaaaccatat cgccatatat catgggtgag ggtggatttc tgttcaatcc ccgcgatgtc
     1021 gacaagaaaa ccctgcttat gatcatcggg ggcgggggaa tccaattcaa catttcagat
     1081 aaattcatcc tgaatgccgg tccgggtccc ggcatacaga tgggtatcga cggaagacgt
     1141 tcattcctgt ccggaaggct tggcctggga tttaaattat catcaccaca cggaggatca
     1201 ggtcttaatc aaatattctc gttccgaaaa taaatctttc cttccaccag gcgtcatcaa
     1261 gggatgtgat ggttacaccc cttgaggaag aagtatgaat aaaggagtta tttcctgtac
     1321 aaataccgga atgtgtaata aaatgcgatg ttttgtagga acggatgaaa aacaccagat
     1381 ccccttttct cagttccttc ctgtcggtga tcagctttcc gtacctggcc tgttcttcgg
     1441 aattatgcgg aaggtttatg ccatatcctg caaacactgc aacaagcagg ccggagcagt
     1501 ccatgcattt catactcttc cctcccatgc agtgcggtac gccaaggtat ttccgggcag
     1561 cccggactat cccgtcagga tttgcccggc gtgtatccag acccttttca gtaccggaat
     1621 caagaaagat ctgcagccgc tgcttatctg cagaactctc aatattgctg aaagagggtt
     1681 cagggtagat tttccgggta caatggctta caagcagctg agttattaaa accagcatca
     1741 gtgcaactat ggttttatag cgtgcaaatg atttcatcag aacagttctg ctgtaagaca
     1801 gcgtaaatat gggaattatt ttcaatgctg ttttatatga ggggtgaaaa agtgtttgct
     1861 gcataattaa tttgatatat ttactctgta ttactgccgc aatcgcggca gcagaataat
     1921 taacttaatt ccaaaatccc gagaaaatga aaagatttta ttccctgagt gtactgatta
     1981 cttttctgtc attatactct ttttcccaga tgattgacaa tgataatctt gtcataaccc
     2041 atggccccta tcttcagaat ctggatgcct ccggtgtcac aattatctgg accaccaaca
     2101 aacctgcagt cccgggaatc agcctgacat atccggatgg cctgaaacgt tttatacgca
     2161 acagccatga cgggataatt gacggaggcg gattattgca caaggtaagg gttgaagggc
     2221 ttgaagccgg aaccacctat cagtacagca tcaattcgat tcagattctt aagtaccagg
     2281 cctacaagat ttattatggc gataccattg acatgaaacc cctgagtttc accacactca
     2341 atccaaaggc ggagaaaaca aattttactg ttgtaaatga tgttcacgag tattcaggaa
     2401 aactggcgtc ctatataaga aacggaaatc ccgccgcaca ggaattcttt atctacaacg
     2461 gtgacatggt taactacgta cagtctccgg aacagctttt tacagggttc attgacacta
     2521 ccgttgcata ttttgccaga acaagggcat ttttctatgc acggggcaac catgaagcca
     2581 ggggctatgc aggaagggaa ttcaaaaatt atttcgactt cagggacaac aggttttatt
     2641 acagctttga tcacggaccc gtccatttcg tggttctcga ctgcggtgaa gacaagcctg
     2701 atgacaaccg ttattactac ggtcttgcag attacgattc ctaccggctt gaacagctgg
     2761 aatggctgaa gaaggaagtg aaaagcgatg cattcagaaa ggcgaaatac aggattgtca
     2821 ttgtacacat gccaatattg aatgaaggtt cacgggggca tggcatgaag tttctggccg
     2881 ataatttcgg accggtcctg aagactgcaa agatcaacct catgctttcg ggtcatacac
     2941 acaggaactc cttccatgcc agaggtgagg caggccagga atatcccgtt ctcgtcaatt
     3001 ccaacaattc attcgtggag atatatgttg acaaggaaaa aatcagggca gttgtcaggg
     3061 atgtaaacgg cggaaacata gcagaatatg aattcagata atattaaggg ccggagcggc
     3121 ccttttttac cggtttcagc tgccatactg aaatagaaca gcacttcctg cgttatagaa
     3181 acggaacagg aacccttgtc cttgcagccg gatgaaatcc ctgcaaagta tcagaagaat
     3241 tcccgaatga aaaagaaacg gaaaataata gtgatctctt ttctgattct tcttctgttt
     3301 ccgcttctct tttttatcgt ggtaatctgc cttgcttcca gaaatctcca gaccagcgag
     3361 gaactccgta attttaaaaa tgccgaggcc tcccgggtac tgtcttccgg cggacaactg
     3421 ctgggcaagt tctatatcga aaaccgcatg aatgtcacat gggggcagct tcctgcccat
     3481 cttgtcaatg cccttgttgc caccgaagac gtgcgcttct ttgatcatga aggcgttgat
     3541 tcccggagtc tttacagggt actctttaaa actctgttgc tgaacaggtc agagtcagga
     3601 ggaggaagca ctataagcca gcagcttgca aaaaacatgt tcggaagaaa agccggcggc
     3661 agcttttcag ttcctgtcaa taagtacagg gaaatcattc ttgccaggag gcttgaaaaa
     3721 acctttacaa aggaagaaat tatcacactc tatctgaata ccgtatcatt cggggaagat
     3781 atatttggca tagatgcagc agcaggaagg tatttcaata aaataacgga aagcctcagg
     3841 attgaagaat cggcaatgct cgtgggcatg ctcaaggcca ccacccgtta caatcccgcc
     3901 ctgaagcctg aaaatgcact gtccaggcga aatgttgtcc tgaggcagat ggagaaatac
     3961 aactatcttg gttcttctga tgccgattca cttacagcgc tgccattaag acttgattac
     4021 aggaaggctg agtccgaagg aagggctgac tattttcttg tgcgggtcag gaaggagctt
     4081 gatgaaatac taggcaatat tgccctgtca accggcagga gatggtgccc ggaaaaggac
     4141 ggactgatta tcacaacctc cctggacgat gaccttcaga gttttgcaag acagtctttt
     4201 gcccgtcacc tttcaaccat gcagaaacac ctgcgcgatc agtacagcac tgctgccggc
     4261 agggcaaaac tcaatgaatt aactgaagcc atactgcttg agaaaaacct tggccacagg
     4321 gccggcaaaa taaaccgcca gctcattttc gactggaaag gatcatatcc tgattccatt
     4381 tcagtcaggg acagcataaa gctggccctg accacccttc atggcggcct gctggcaatg
     4441 gacccgcaat cgggagccat aagggcatgg gttggaggca tcgactttat cacccagccc
     4501 tatgaccaga tacttgccag gagacagctt ggttcggttt tcaagccggt attctatgcc
     4561 cttgcctttg aggagggaat gcacccctgc cagtatctgg acaatgacag ccttgtactc
     4621 cgggattatc aggactggag gccggaaaat ttcgaccgca gctacggtgg gaaatactcc
     4681 gttgccggag ccctggccag atccatgaac ataccctctt tcgtcctttt ccgggaactg
     4741 gaatttgaaa aactaaatct cctctggaag gaaatgggat tcaccttccc cctgaggaac
     4801 actcccgctc ttgcgcttgg aacggctgaa gccagcctgg aagaaattgc ggtggcttat
     4861 tcggtatttg caaacggagg atttaaaata aaaccatggt cggtaacctc tgtaattgaa
     4921 ccggatggca atattattta tctcaggaac agggatgaaa tttttcccag gatcctcagt
     4981 gaaagatcct gtcatctgat aaacgccgtt ttacagaaag cagtaaggga aggaaccgga
     5041 acatccatgc aatcggttta tggcgtaagc tccgctttcg ctggaaaaac cggaacaacc
     5101 cagaactatg ccgatgcatg gtttgcagct tacaacccgg gattgacggt tgtggcaaga
     5161 acgggtgcct cactgccatc catacatttc aacaaccggt accacgggac aggcagtgtc
     5221 ctggccctgc cgctggtggc cctgactctc aaaaaggctg aatcccgtcc cgtaacagcc
     5281 ggccgcttta taaaaccttt ccctcctttg ccgcccgaac tgcagggatc actcgactgc
     5341 cccgacttta tagaggataa acttcctttc cgccagacgg acaggatcag aaaacagaga
     5401 actgacagaa attacggaac tgacaggagg aaaagcttct ttctggaaag actgttcagg
     5461 agaaacaggc ggaactgaac tggtatgatg acatcattcc ggaagtacca gcgacagaca
     5521 tttcagccag aagttgttgt aatgctccca gtacacaaaa ttaagacccc agtgcggggc
     5581 agggtcggaa gtataggcca gcacccttcc cctgccgatt ttccttgtag caaccagcgg
     5641 atgccctgtt tcctcaatag tcaccagcac ctccccttcg ggaattactc tggtttcatt
     5701 atatcccagt atcggaggca tcccttcgaa atccgattca aaaaacacct tctttccggc
     5761 atctgtcagt ttcggataaa atccctcggt gctttccacc agatcctcat gcggaaggca
     5821 ttcaacagga agtattgatc tgaattctgt tcttccccag cctccctttc ccagttcacc
     5881 ggtaaaggaa taccatccgc ccagcatcat aagtcctttc cctgctgcaa cagcttccgc
     5941 ggtaagcctg atcctgtcag gataaaccag tatctttttc ccaaactcct cccgtacgaa
     6001 gaaatgcgga gccagctgga aaagtttccc ttcaatatca ctgaaaataa ttacatcata
     6061 ctcttccagg attttttcat attcacccgg accaagttta tagaaatccc agccgggaac
     6121 tgaatcaacc tgatgtttcc cgtcagattc cagagcttct ttgagccact ttccgtaatt
     6181 gaaaatttcc agtcccttcg gagcaaactg gaatggtgat tctgcaaatg taggtcctgt
     6241 aagaaccgcc cagtcacctg catagtaaat tttagccata attgtaatac tgaatttgaa
     6301 aatttatccc taatagaaaa actgcatttt aaatgtacat gaaaaatcct tattaacata
     6361 cagaagatga aaaatccgtt ccgcacctta aggttcctga gcgcccggga acgcaggatg
     6421 cagctccgga aagcaccatt acatacctca ttttgcatcg gcggttatca tcaatattgc
     6481 cggacatgtc aggaaacccg cagatgagca cgatctgaat catcagtttt gtcagtaaca
     6541 attgtgattg caggaacaga aacaggttaa aagcctttga gaacagaaaa aaaaaattga
     6601 cttacaacat tttctgtcgt aaatttatta ttgtaattaa tgataccgaa atgaaaaaca
     6661 gacaaagaat tttatctatt ctgttcctga ccctgttcag catatcatac ctgcagggtc
     6721 agcaagccgt tgtcacgagc ggcggaattg caaccgggac cacaggatca gtcagctaca
     6781 cagtagggca gatagcctat cagactttta cagggaccgg cggaacctta cagcagggtg
     6841 ttcaacagcc ctgggaggta tcaactccgg ttgctgtagc agaaacggag gatatctccc
     6901 tgttgatgaa tgtttatccc aatcctacaa gcggggcttt taagctcatt gtcggtactc
     6961 tggaaaacag aaacctgaga ttcaggttgt acgacatgaa cggtctgctc cttcaggata
     7021 aaaagatcga cagcgaggaa accgaaatat tcctgcagga tctttcatcc tcaatgtatt
     7081 ttcttaaaat aataaacaac gccagtgaga tcaaggtttt caaaattgtc aaaaagtaat
     7141 tgcatatgaa gaaattattt accctcatgc tgatggtatt actgaccatt accattcttg
     7201 ctcaggctcc ttcaaaaatg agttaccagt ttgtaataag aaacacaagc ggatcactgg
     7261 ttgtaaatag cccggtcggc gtaagaattt caattctgca gggctcgccc gcgggagcac
     7321 ttgtttaccg ggaactgttc aatcctaacc cgcaaacaaa tgcaaacgga ctcgtttcca
     7381 tagagatcgg aacaggattg ccaatcacag gcacattctc ttcaattaac tgggcaggag
     7441 ggccgtatta tctcctgacg gagactgatc cctccggcgg gacaagctat actgttacag
     7501 gcaccagcca attactgagt gtgccatatt cattgtactc cgcaatgtca gcaagcgtat
     7561 ccgacaacag cataacctct gccaagatag caaatggtac aataatcgcg gctgacctgg
     7621 ctgacggctc agttacttca gccaagatag ccgacggcac catagctact tccgatatcg
     7681 ccaataatgc tgtaaccggg gcaaagattg ccgatgagac tataactgca gccgatattc
     7741 agaacagaca aagaaatatt gttttccctg ccaatgctct caactacgac aaaggatcat
     7801 caattctgac tcaggatgcc tatggaatat attggaaatc caattttgct gatggggctt
     7861 atctgactat tccccgaccg cttgactggg tcggaacaag taatgtcatt ttgaagctcc
     7921 attttatgaa aggcggtgct acaggcggag ttgtgaattt ttttatcagg ccccgttcat
     7981 ataaccccgg tgattcatac gcagatgcgc cggcccagtt ccctgatagt cccgttactg
     8041 ttgctgccgg caccgatcat gggatatata ctcagacgtt tacgatacct tactcaaaat
     8101 tcggaacaaa aaacctgtgg gtaattacaa tacagaggaa gggtagcgga gaaacattca
     8161 caggtgatct gatattaatg gcagtagaga ttgtttacat ggcagaacaa taagtcctga
     8221 gctatgatta atctgatttt tacctgtcag gaaattgatt gtcatcgtat tacaacagat
     8281 aattatttca atgtaaaatc agcactagct atgaaaaaat ttattattct gctggtggtc
     8341 ttgt
//
LOCUS       DAOSPX010000123         8314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8137_length_8314_cov_5.392784, whole genome shotgun sequence.
ACCESSION   DAOSPX010000123 DAOSPX010000000
VERSION     DAOSPX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8137_length_8314_cov_5.392784"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PLZ75_07375"
     CDS             complement(<1..1193)
                     /locus_tag="PLZ75_07375"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOS72251.1"
                     /translation="MKNIYHSALIFILAVTMPSGICSQERYPASAFIKADHLEAHVSF
                     LASPLLNGRKNGSPGLDIAQEYIVSNARLLNLKPAAGTGYLQPYQVISKGVDQKKSGI
                     RISSPDDDTVRITKNLYQMFPFHPADITVEGEVVFAGYGLVREEYGYDDFRDIPTEDK
                     ILLVMTRSPTKADGSTYIFEGDNWSDFSSYTFKFRNLFRLKAKAVIFVCDPKSGFNSL
                     DEQEPGIAEYLSSSLSLKGKKAPVFPFPGTMKIFFADRSVADQLLEGSGRNLEDIQKK
                     IDTDLQPCSFAIPGKSLAIDIVTSTEEITLNNVAAIIEGSDPVLKNEFLVYSCHADHI
                     GGYGDRINTGADDNASGCAALLSIAGAFRELERKPLRSILFLWFSGEEIGLFGSEYYT
                     DNPLVP"
     gene            1454..3958
                     /locus_tag="PLZ75_07380"
     CDS             1454..3958
                     /locus_tag="PLZ75_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS72252.1"
                     /translation="MNSKKQKPAVPWPLIILFLVLSAGELVVGLLYYRYHRMHMFNDT
                     KQELSAIANLMVRTLSQWRSERINDGNYLNESAMLSEQFFRFSNNQKDSKLREELREN
                     LRVLVDNYDYSSALFVDNNLKVRLNYPMEDSLPGDYLKRNLPAFMKRGEVRMTDIYRS
                     AEMNYHHIDLIIPLRDGNNSPGQVFGALVLRIDPREVLYRLIRTWPLPSRTSEAYLVR
                     QDGDELVYLSNLKYSDSTDLLRKSITEEKLAGTMALQGIQESDEAVDYRGVRVIAAMK
                     KIPQLPWFMVAKVDRAEVLGRLNFEFRQILMITFLFILITALLLGMLWWNQRVRFYRT
                     RYETELEHLALVRHFDYILKYANDVIMLFNKDLKVIEANDQALEKYQYKRNELIGKRL
                     PDLLPDRKSGRHKDVISILDREGHHTFEELHKRKDGTTFPVEISARRIMMEGLVYYQT
                     IGRDITERKHSEETLRASEERFRKIFEESPIGMAMTGKDLVVIRANSAFCSMMGYSEE
                     ELMGMTFRSFTHPDYVGKDEYGLLRLIANEIPIYHSENEYIRKDGRMIWGSTTVSVVR
                     NRDEEVQFFLGMVEDVTSRKIAEAELIAAKEKAEESDRLKTAFLHNVSHEIRTPMNAI
                     LGFSSLLSEPGITDAEKNQYIDIIFQSGNQLLSIINDIVDLAGIESGHVKIKIGRVNI
                     NSVLREMSEQYAYKVKSQNIGLNLEMPLPDEESETMTDRTRLIQILSNLLTNAFKFTK
                     TGEIDFGYRLENGFFEFYVRDTGIGIPGEFHSLIFERFYQVDSTVSRQYPGTGLGLSI
                     CKAYVGLLGGNIRVESESGQGSTFYFTIPYNREGID"
     gene            4245..5393
                     /locus_tag="PLZ75_07385"
     CDS             4245..5393
                     /locus_tag="PLZ75_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOS72253.1"
                     /translation="MKKRSIDEINEKIRGGKAVVLTAEQVAAMGKEMTPAEIFSRVDV
                     VTTATFGPMCSSGVFINFGHTTPPIRMERITLNNVPAYGGIAAVDAYLGATETAMPND
                     QYGGAHVIEELISGKDVLLEASAKGTDCYPRTEVKTLINKDTINEMIMFNPRNAYQNY
                     NVAVNSTHKIKYSYMGTLLPSLGNANFSTSGELSPLLNDPELRTIGIGTRILFGGTTG
                     YVAWHGTQFNTGKPVNEYGIPVGNSATLALIGDAKQMSTRFIKAAYFERYGVSMFVGV
                     AVPVPVLDEDLARRVSVNNRQIETNVIDYGSGNFEVLGRVDYDSLFSGRITVNGKKIR
                     TAPLSSVRTARELADILRLEISEGRFFLTEPIALFKKTSGLNSLEIRL"
     gene            5419..5829
                     /locus_tag="PLZ75_07390"
     CDS             5419..5829
                     /locus_tag="PLZ75_07390"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HOS72254.1"
                     /translation="MASKRFVLTFPPDATGQPITYNLIKKFDIMINIVKADVSPGKIG
                     HLVIEMTAPARVLKEGMEYIKQQDIQCDPIEKKITYREDLCIHCGACTAVCFAGALTM
                     RRDNAELLFEPEKCVVCELCLKACPLKLFSIDIG"
     gene            5822..6580
                     /locus_tag="PLZ75_07395"
     CDS             5822..6580
                     /locus_tag="PLZ75_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOS72255.1"
                     /translation="MVKNTSYQPRLYRESMGNDRWQVFRSLYRETDLWVAAGREYSLT
                     DVEAFTKQRVIYYRNILEDHIRRQPGFKTSLVPVSELTDTEPLIAEMYRASSAALTGP
                     MAAVAGAIAQFVCNDLTDEFSLGEVVVENGGDIFLKLEDEAVISVYAGTSPLSGKVSL
                     LVKPEETPLSVCTSSATVGHAFSTGKADACMIACRSGALADAYATSFCNKVKSRDQVQ
                     ETAEEALKKPDILSVVIIMDDVVGLGGRLSFQFA"
     gene            6774..7184
                     /gene="rnk"
                     /locus_tag="PLZ75_07400"
     CDS             6774..7184
                     /gene="rnk"
                     /locus_tag="PLZ75_07400"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOS72256.1"
                     /translation="MKNLIMNRVDHARILKCINDARDMNTISSEEAENLLHELNSAKI
                     VEPKDVPVNLVTMNSIVKIRFINTDKQIQFRIVYPDQADLKENKISIFSPVATALIGY
                     KVNDEIEWLIPSGMTTIRIEEIIYQPEAAGDYTL"
     gene            7250..8185
                     /locus_tag="PLZ75_07405"
     CDS             7250..8185
                     /locus_tag="PLZ75_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOS72257.1"
                     /translation="MSKYRIVTIDGGGIRGIITTILLQRIVATPGLENFLETIDLLAG
                     TSTGGLLSLGISHGISLERIRELYEKSGPEIFADTCLDNIRDLGKLLGADYSIRRLRR
                     ELNDIFGSTTLGQLKKRVLITTFDLDNEDQDPAGRTWKPKLFHNFPGENSDEDAQAAD
                     VGLYTSAAPTYFPSVDGYIDGGVYAVNPSVCALAQTQDRRYRPNPPLRNILLFSLGTG
                     TSLQYIKGKSLDWGYLQWIKPLIDLIFDGTAGIADYQCRQFLGPDYHRLAPVFPPGVK
                     IPMDGVKKIPEMISFAESLDMESTLRWLKETWNKE"
BASE COUNT         2359 a         1735 c         2065 g         2155 t
ORIGIN      
        1 ggtacaagag gattgtcggt atagtattcg gatccgaaca atccgatctc ttcgcctgaa
       61 aaccacagga acaatattga tctcaggggt ttcctttcaa gttccctgaa agcaccggct
      121 attgaaagga gagctgcaca ccctgaggca ttgtcgtcag ccccggtatt tatcctgtca
      181 ccgtatcctc cgatatggtc ggcatgacag gaatatacaa gaaactcgtt tttcagtacg
      241 ggatcgcttc cttcaataat ggcagctaca ttgttaagag ttatttcttc cgtggaggtg
      301 acgatatcaa ttgccagact cttgccgggt atggcgaacg agcatggctg taaatcagta
      361 tctatctttt tctggatatc ttcaaggttc cttccgcttc cctcaagcaa ctgatcagcc
      421 acgctccggt cagcaaaaaa gattttcatc gttcccggaa aggggaacac aggagccttt
      481 ttgcccttga ggctgaggga tgaactcaga tattctgcaa ttcccggttc ctgttcatca
      541 agactgttaa aaccggattt gggatcacag acaaatatga cggcctttgc cttgagccgg
      601 aaaagatttc tgaatttgaa agtgtaactg ctgaaatccg accagttgtc accttcgaag
      661 atataggtgc tgccgtcagc ctttgtaggg gatctggtca ttacgagcaa tatcttgtcc
      721 tctgtcggga tatccctgaa atcatcataa ccgtattctt ccctgaccag accgtagcct
      781 gcaaaaacaa cttccccctc gacagtgata tccgccggat ggaaagggaa catctggtac
      841 aggttcttcg ttatccttac agtatcatca tccggggaac taattcttat tccggacttt
      901 ttctggtcca ctcccttgct tataacctga taaggctgca ggtagcctgt tccggcagca
      961 ggtttaaggt tgagcagtct ggcattggaa acaatatatt cctgggcaat gtcaaggccc
     1021 ggggatccgt ttttccttcc gttcagaagc ggagaggcaa ggaatgaaac atgtgcttcc
     1081 aggtggtcag cttttatgaa agcggaagca ggataccgtt cctgggagca tatgcccgat
     1141 ggcattgtaa cagccaggat gaaaatgagg gcagagtggt aaatgttttt catgagcgga
     1201 atgactgatt atttatcagc ggggcaaatt taatgaaagt ttcagattcc tttgcaggat
     1261 ccggtgtgca ggcctttttt ctggccagct ttcagatctg tgatattacg gcagaaaatt
     1321 gtaatgacac cggcattccg gctcacggga atattgcgct gcagaaattt aatattaatt
     1381 atcgggaaaa tattaaattt acggcattgt tgaaagtgcg ggtttgattt tcctgtaaga
     1441 gaagaaaata ataatgaatt ccaagaaaca aaaaccggcc gttccatggc ctcttattat
     1501 tctctttctg gtattgtcgg cgggtgaact tgttgtcggg cttctgtatt acaggtatca
     1561 cagaatgcac atgttcaatg acaccaaaca ggaattgtcc gcaatcgcca atctgatggt
     1621 cagaacgctt agtcagtggc gcagtgagcg gatcaatgac ggcaattacc tgaatgagag
     1681 tgccatgtta tcggagcagt ttttccgttt cagcaataat cagaaggaca gcaagctgcg
     1741 tgaagagctc agggaaaatc tcagggtact ggttgataat tatgattatt cctctgcatt
     1801 atttgttgac aataatctga aggtgagatt gaattacccc atggaggatt cgcttccggg
     1861 ggattatctg aaacggaacc ttcccgcttt catgaagagg ggggaagtca ggatgacaga
     1921 catttacaga tcagcagaaa tgaactatca tcatattgat ctgataatac ccctcaggga
     1981 cggaaacaat tcccccggtc aggtatttgg tgcccttgta ttaaggatcg atccccggga
     2041 ggtactttac cgcctgatac gcacctggcc gcttcccagc cgtacttccg aagcctatct
     2101 cgtaagacag gacggagatg aattggtgta tctcagcaat ctgaaatatt ctgacagtac
     2161 ggatctgctg aggaagtcca taacggagga aaagctggca ggaacaatgg cgcttcaggg
     2221 tattcaggaa agcgacgaag ctgttgatta ccggggggtc agagtgattg ctgccatgaa
     2281 aaagattccc cagctgccct ggttcatggt ggcaaaggtt gaccgggcag aggttctggg
     2341 caggctgaat tttgagttcc ggcagatact catgataacc tttcttttca ttctgataac
     2401 tgccctgctg ctcgggatgt tatggtggaa ccagcgggta aggttttacc gtaccagata
     2461 tgaaacagag ctggagcatc tcgcccttgt caggcatttt gactacatac tcaaatatgc
     2521 aaatgacgtt atcatgctgt tcaacaagga tcttaaggtt attgaagcaa atgaccaggc
     2581 tcttgaaaaa taccagtata aaagaaatga gcttattggg aaaaggcttc ctgatcttct
     2641 gcctgacagg aaatcaggaa gacacaagga tgttatcagc atacttgacc gtgaggggca
     2701 tcatacattt gaagagcttc acaaaaggaa agacggtacc acatttcctg ttgagatcag
     2761 tgcccggagg ataatgatgg aaggactggt atattatcag accattggca gggatatcac
     2821 cgaaagaaag cattcggaag aaacactgag ggcaagcgag gaaagattcc ggaaaatttt
     2881 cgaggaaagc ccgataggaa tggcaatgac aggcaaggat cttgttgtaa taagggctaa
     2941 ttccgctttc tgcagtatga tgggatattc cgaagaggaa cttatgggaa tgacattcag
     3001 aagttttacc catcctgatt atgtcgggaa ggatgaatac ggacttttgc gactgatagc
     3061 caacgagata cccatttacc attcggagaa tgaatacata aggaaggacg gaaggatgat
     3121 ctggggttcc acaacggtaa gtgttgtaag aaacagggat gaggaagtac agttctttct
     3181 gggaatggtg gaggatgtga cttccagaaa gattgccgaa gctgagctta ttgcagccaa
     3241 ggaaaaggcg gaggaaagtg acaggctgaa aacggcattc ctccataacg tgtcccatga
     3301 gatacgtacc ccgatgaatg caatactggg tttttcttcc ctcctgagtg aacccgggat
     3361 tacggatgct gaaaagaacc agtatattga cataattttc cagagcggaa accagctgct
     3421 ttccataatc aatgatattg ttgacctggc cgggatagaa agcggacatg tgaaaatcaa
     3481 gatcggcagg gtgaatatca attcagttct cagggagatg tcggagcagt atgcctataa
     3541 ggtcaaatcg cagaacatag gactgaacct ggagatgcca ttgccggacg aggagtcgga
     3601 aacaatgaca gacaggacaa ggctgataca gatactttca aatctgctga ccaatgcttt
     3661 taaatttaca aagaccgggg aaatagattt cggatacagg ctggaaaacg gcttttttga
     3721 gttctatgta agggatacag gcattggtat tcccggcgag ttccattccc ttattttcga
     3781 aagattctac caggttgaca gtacagtttc ccggcaatat cccggtaccg gccttggtct
     3841 ttccatctgc aaggcctatg tcggattgct tggcgggaat atacgggttg agtcagaatc
     3901 aggacagggc tcgactttct atttcaccat cccgtataac agggaaggca ttgattaagc
     3961 cgcatggagg cagggtctgc cttgttttcc ggatttccgg ttctggctcg attccatcgc
     4021 gcttaacgga aatgccttgc agaacttccc ggattccgcc aggaatattt tcatcctttt
     4081 ttgtggaatc agcatgcagg gtttaatact ccgcggatga taaatagatt atgaaaaatg
     4141 atttctttcc aaaaaagtga tgaaaaataa aaatgaattg attaaggggt ttatctttga
     4201 cacttgatct gtgattttaa ggattctgta ataattaaac tatggtgaag aaaagaagta
     4261 tagacgagat caatgagaag atccgcggcg gaaaggcagt agtgcttact gctgaacagg
     4321 ttgcggctat gggaaaggaa atgactccgg cagagatatt cagcagggta gatgtggtta
     4381 ccactgccac attcggaccc atgtgctcgt cgggagtttt tatcaatttc ggacatacca
     4441 caccaccaat ccggatggaa agaataacac tcaacaatgt tccggcctac ggggggattg
     4501 ctgcggtaga tgcctatctg ggtgcaaccg agacagccat gcccaatgac cagtatggag
     4561 gggcccatgt gattgaagag cttatttcgg gaaaggatgt tcttctggag gcaagtgcga
     4621 agggaaccga ttgttatccc cgtactgaag taaagaccct tatcaataag gatactatca
     4681 atgaaatgat aatgttcaat ccccggaatg catatcagaa ttataatgtg gcggtgaaca
     4741 gtactcataa gataaagtac agctatatgg gtaccctgct cccttccctg gggaatgcca
     4801 atttttccac ttccggagag ctgagtcctc tgctgaacga tccggagctg cgcactatcg
     4861 gcataggaac aaggatcctt ttcggcggca ctacgggtta tgtggcatgg cacggaactc
     4921 agttcaatac cggcaagccg gtcaatgaat atggtattcc tgttggtaat tctgctaccc
     4981 tggctcttat aggagatgcg aaacagatga gcaccaggtt tatcaaagca gcatatttcg
     5041 agagatatgg tgtttcgatg ttcgtcgggg tcgctgttcc ggtacccgtg cttgatgaag
     5101 atctggcaag aagggtctct gttaacaaca gacagataga gaccaatgtc atagattacg
     5161 gatcgggaaa ttttgaagtt ctgggacgtg ttgactatga ttctcttttt tcagggagga
     5221 tcaccgtcaa cggaaagaag attcgcacag cacctctgtc aagtgtcagg acagcaagag
     5281 agctggcgga tatcctcagg cttgaaataa gtgaaggacg gtttttcctc acggaaccaa
     5341 ttgctctttt taagaagaca agcggactga acagcctgga aataagactt tgatgtttta
     5401 attttttaat ttcagattat ggcaagcaaa agatttgttc tgacttttcc tcctgatgca
     5461 acaggacaac caatcacata taatctcata aagaagttcg atattatgat caatatcgtc
     5521 aaagccgatg tttcgcccgg caagatcgga catcttgtca ttgaaatgac agcaccggcc
     5581 agggtcctga aagagggaat ggaatacatt aaacagcagg atattcaatg tgatcctatc
     5641 gaaaagaaga tcacttacag ggaagacctc tgcattcatt gcggggcatg tacggcagta
     5701 tgctttgccg gagcacttac catgagacgt gacaatgctg aactattgtt cgaacctgaa
     5761 aaatgtgttg tctgtgagct ctgtctgaaa gcctgtccgc tgaaactttt ttccatagat
     5821 attggttaaa aatacttcat accagccaag gctctaccgc gaaagcatgg gtaatgacag
     5881 atggcaggtc ttcagatcgt tataccggga aacagattta tgggttgcag caggccggga
     5941 atattccctg acggatgtgg aagcctttac gaagcagagg gtcatatatt acagaaatat
     6001 tcttgaagac catatccgga ggcaacccgg atttaaaaca tcccttgttc cggtttcaga
     6061 actgactgat acggagcctc ttattgcaga aatgtacagg gcatcctcag ctgcccttac
     6121 cggacccatg gctgcggttg cgggagccat cgcacagttt gtatgcaatg atcttactga
     6181 tgaattcagc cttggggagg tagttgttga aaatggcgga gatattttcc tgaaacttga
     6241 agatgaggcg gtaatctcgg tttatgcggg aacatcacct ctttccggaa aagtcagtct
     6301 gctggtgaag cctgaagaaa cacccctttc tgtctgtact tcttcagcaa cggtcggcca
     6361 cgctttcagt acgggaaaag ccgatgcatg catgattgca tgccggtcag gagcattggc
     6421 tgatgcatat gccacgagct tctgcaacaa ggtaaaaagc agggaccagg tacaggaaac
     6481 agcggaagaa gccctgaaaa aacctgatat tctttctgta gtgatcataa tggatgacgt
     6541 ggtcggatta ggcggaaggc tgtcatttca atttgcttaa ccgacaattg ttgagtatct
     6601 ttaaatactt cattccatag ggaggtaatg gaaccctgaa ttgctgacag gcacggcagg
     6661 gcagcggaaa ccttgagaag ttcccggctt gctgactcag gtgacgggat gctggaaatt
     6721 cccggtgctt ccttataatt ttaactattg gcaacaggat aacttctttt cggatgaaaa
     6781 atttaattat gaaccgggtg gatcatgcca ggatattgaa atgcataaat gatgcaagag
     6841 acatgaatac catcagttca gaggaagctg aaaatctcct ccatgaactg aattccgcaa
     6901 aaattgtgga gcccaaggac gttcctgtca acctggtgac gatgaattcg atagtgaaaa
     6961 tcagatttat caacacggac aagcagatac agttccgcat agtatatccc gaccaggctg
     7021 atctgaagga aaacaaaata tcaatttttt ctccggtggc tacagcgctt atcggataca
     7081 aagtcaatga tgagattgaa tggctcattc cttcgggcat gactacaatc agaatagagg
     7141 agattattta tcagcccgag gctgcgggag attatacatt gtaaattgca tgtctgtctc
     7201 tgctttcagc agatccggac agaagttcat gtgaataatt tataaatcca tgtcaaaata
     7261 cagaattgtt accattgacg ggggaggaat acggggaatc attacaacaa ttcttctcca
     7321 aagaatagtt gcaacccccg gacttgagaa ctttcttgaa accattgatc ttctggccgg
     7381 aacctcaaca ggaggtcttc tttccctggg catttcccac ggaatcagcc ttgaaaggat
     7441 cagggaacta tatgaaaaaa gcggcccgga aatatttgcc gatacctgtc ttgacaacat
     7501 ccgggacctg ggaaagctgc tgggtgctga ttatagcatc aggcggctca gaagggaact
     7561 aaacgatatt ttcggcagca ccacactggg acagctgaaa aaacgtgtac tgatcacaac
     7621 ttttgatctt gataatgaag atcaggatcc cgccgggaga acctggaaac ccaaactctt
     7681 tcataatttc ccgggtgaaa acagcgatga ggatgcccag gcggccgatg tgggccttta
     7741 taccagtgct gcaccaacat atttcccttc tgtggatggt tatattgacg ggggagtgta
     7801 tgcagtcaat ccttcggtat gtgcacttgc ccagacacag gacaggcggt acaggcccaa
     7861 tcctccgctt cggaatattc ttctgttttc cctcggaacg ggaaccagtc tgcagtatat
     7921 aaaggggaaa tcactggact gggggtatct gcaatggatt aaaccgctga tagacctgat
     7981 atttgatggc acagccggga ttgctgatta tcagtgccgg caattcctcg gaccggacta
     8041 tcatcgcctg gctcctgtct ttccgccggg cgtaaaaatt ccgatggatg gtgtgaaaaa
     8101 gattcctgaa atgatcagct ttgccgagtc gctggatatg gaaagcactc tcagatggct
     8161 gaaggaaacc tggaacaagg aatagaaata tgctgaaaca gatttgaaat taattgaaac
     8221 ccggcagcca ttacactcat cagccttaat gtttataaac tgaacacaat ctgaaccatg
     8281 aaaagattac tgtctgcact gctgttgtta atca
//
LOCUS       DAOSPX010000124         8263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8193_length_8263_cov_4.707481, whole genome shotgun sequence.
ACCESSION   DAOSPX010000124 DAOSPX010000000
VERSION     DAOSPX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8193_length_8263_cov_4.707481"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLZ75_07410"
     CDS             complement(<1..770)
                     /locus_tag="PLZ75_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72258.1"
                     /translation="MGHIYRPVLVSCLLLISVALSSQTHNWTIDPQDYESDCEVNAVV
                     FMGSDEVTTGTLGAFVGNTCRGFADGIFFPGTGRTIFSVRCYSNEPENPETFTFRYFD
                     PAGGSYYNIIETVVFVSDGQLGNARNPLLFHRCDPVALLSGPLNGRMCAVSGSTAFSV
                     TAGGTSPYTYQWQYNTGTEWVSTTNGIPAGAVYSGSNTATLNVSGITSAGQYQYRCHI
                     SNCHEWSSITSNSATLTVDPLPGAAGTISGLTTVCQGQS"
     gene            complement(793..>2869)
                     /locus_tag="PLZ75_07415"
     CDS             complement(793..>2869)
                     /locus_tag="PLZ75_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS72259.1"
                     /translation="QNPVIPNVTTAASGTYSVAVTVDGCTSEPGTTTVVINAIPATPV
                     PSNNSPLCAGGTLNLTTSDVTGGIFNWTGPDGFTSTQQNPSIANVTTAASGTYSVTVT
                     VAGCTSNAGTTSVLINPLPSAITGYNYTAAGSSTTLSNTSPGGSWSSSVPSVAEINPS
                     SGEVRGMTAGTALITYTLPTGCAVSMTFNVLPSGWEFDSENFTHQGRITSSVTIEGTG
                     AESGFLAAFAGNECRGITEAVYNPQSGNYIFELNCFSNTETGDVLVFRYFNPAVVMEY
                     NLDRSVDFSSGMNTGSASAPFPMNIGTDYSVSLSAGWNWFSFNTTLDNMTPQFILEAA
                     NSPGDYIKNQTVSATYYEGLGWFGTLDRLDSRELYKIRLQNNSVIHFSGRPVDINAIN
                     IPLASGWNWIGYLPRQAMPVNTALQSLSPSDLDYIKDQVSSSTYYADFSLWYGTMDDM
                     EPQRGYMLRVTNPGTLVYPEPEGKGNQMSSGTEQTGAFNPHPYEFSGEVFVRVILDDI
                     PKGSENDTLYAYVGDELRGLTAGMEFLDGTYHFSLMIYSNLVTGETVTFRYYNSDTKK
                     IYNCSETVIFTDDMRLGTAQYPLLMHAYATSVHENIINDPDFRIYPNPSEKFLNIEYT
                     LNEPGDVRITVFDSFGRAVRTLVDQKQQPGSYLYEWNSDLPAGGVYFIKLDGGKRQKI
                     RKIILLGQN"
     assembly_gap    2870..2969
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2970..3725)
                     /locus_tag="PLZ75_07420"
     CDS             complement(<2970..3725)
                     /locus_tag="PLZ75_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72260.1"
                     /translation="MFLFLNAELYGQECDNPSVLVLGAASGSTCGTHPLTVENNEFGG
                     GAISVTISTNGSGTLDQEIIDVSPFSFTYNPAVADAGSTVTITVTANNPDQECEVLTS
                     VFLLHVNTVPDTPSASNNGPLCEGETLNLFTSTEIEGIYNWTGPDGFTSTQQNPSIAN
                     VTTAASGTYSVTVTVAGCTSNAGTTEVRVNAIPQPPDVKNNSPICEGETLLLAASDVP
                     GGSFYWTGPNGFTSTLQNPVIPNVTTAASGTYSV"
     gene            4454..5323
                     /locus_tag="PLZ75_07425"
     CDS             4454..5323
                     /locus_tag="PLZ75_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOS72261.1"
                     /translation="MKKNKSITVLAVIAAFIIAAGSNTYGQSKKETPFNIITYNIRLN
                     TSSDGENAWPFRKDRVAGLLKFHRADIFNVQEALPEQMDDLVAAFPDFGHVGVGRDDG
                     KREGEHMSVFYSKKRFTKLDDGMFWLSETPEKPGLGWDAACNRTCTWIKLKDNITGKK
                     FLVFNTHLDHRGNKARQEGVRLILERMALINKENLAVIFTGDFNLAKTAAPIQSVLKV
                     LDDARDKSLTQHYGPDGTFTGFPVKANLTGAIDFIFINNKVKVLRHGTLTDSFNLYHP
                     SDHLPVLAEVQID"
     gene            complement(5386..6279)
                     /locus_tag="PLZ75_07430"
     CDS             complement(5386..6279)
                     /locus_tag="PLZ75_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS72262.1"
                     /translation="MKNELSRRQFIQRTLGAGVALAALNALPAAAMDRKRPKGLQMGL
                     VTYMWGAKWDLPTLISNCEKSGLLGVELRTQHAHKVEIDLTPAQRKEVKKRFADSPVT
                     CVGYGSNYEFHSPDPARLRENIEGAKEYVKLCKDIGATGLKVKPNTLPKEVEREKTIA
                     QIARSLNELGKFARDYGQIIRVECHGPVTQEIPNMKAIFDQVTEKNVKMCWNSNDVDL
                     NPPGLEANFNSVRQWIGDTTHIRGIKISDYPYEELFRLLHGINYRGWILVEATTPYTD
                     PVAVMREQLTLFNEIMDRIKS"
     gene            complement(6409..7473)
                     /locus_tag="PLZ75_07435"
     CDS             complement(6409..7473)
                     /locus_tag="PLZ75_07435"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS72263.1"
                     /translation="MKNTAVRASMPVLILLVSLSCNTKRSARNDSSKIFFVRNETERK
                     VDVFIGKDHFTSFCWPENVYKPILYPVKTAAGTEVTRGFPLVPREGERNDHIHQVGIW
                     LNYGKVNGLDFWGNGHRGMKEPGGGEIKHLSIEKMEEKNGEAILLTKESWVDPDGVEL
                     LAENTEYHFLAEESLRIIDRLTTLKAGSFAVNFDDTKEGMFGIRVARQLELPYDDMVT
                     VLGPDGKPLPEKVRAGDVATGNYRSSEGKSGDDVWGTRAKWMNLYGNIGEEKISLVVC
                     DHPDNPGYPTYWHARGYGLFAANPLGWADFTGGRERLDFSIRPGEEITFRYRVIISSG
                     IHLTDAEINKLADEFAGRYK"
     gene            complement(7745..>8263)
                     /locus_tag="PLZ75_07440"
     CDS             complement(7745..>8263)
                     /locus_tag="PLZ75_07440"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72264.1"
                     /translation="GIDPKECAQETPNTQASVFKYADGTILEFETRGRFTNAESGLGI
                     QIGNMFLGTEGYVEINGSEWKAYRQRERQPFAGSDIGERPVDLMKAPGGGSHYVNFLE
                     AIRAGNNGPLHCDILEGYMSSCLPSLANISYRLGRELTFDGTKEQFVKDKEADKMLTR
                     EYRKPYVVPAVV"
BASE COUNT         2163 a         1944 c         1785 g         2271 t
ORIGIN      
        1 gactgaccct gacagacagt tgtcagtcca ctgattgtcc cggccgctcc cggcagggga
       61 tccaccgtga gagtggcaga atttgaagtg atactgctcc attcatgaca attgctaata
      121 tggcatctgt attgatactg accggcagat gtgattcccg aaacatttaa agttgccgta
      181 tttgacccgg aatagactgc accggccggt atgccgttgg ttgtgcttac ccattccgtt
      241 cctgtgttat actgccactg gtatgtatag ggagaagtac ctcctgctgt cactgaaaac
      301 gctgtgcttc ccgatactgc acacattctg ccgttaagcg gtccgcttaa caatgcaacg
      361 gggtcacagc gatgaaacag cagaggattc cgggcatttc ccagctggcc atcagaaaca
      421 aataccactg tttcgatgat attgtaatat gatccgccgg caggatcgaa atatctgaac
      481 gtgaaagttt caggattctc cggttcattg ctgtagcatc tcacggaaaa tatggtcctc
      541 ccggtgcccg ggaaaaatat tccatctgca aaacccctgc atgtattccc cacaaatgct
      601 cccagggtgc ctgttgtgac ttcatctgag cccataaata caaccgcatt tacctcacaa
      661 tcagattcgt aatcctgcgg atctatggtc cagttatgtg tttgagagga tagggcaact
      721 gatatcagca aaaggcatga taccaatacc ggcctgtaaa tgtgtcccat agttatgttt
      781 ttaaaagttt ggtcaatttt gtcccaggag aatgattttc cgtatttttt gtctcttccc
      841 tccatcaagt tttataaaat aaacaccccc ggcaggcaga tcggaattcc attcatataa
      901 atagctgccg ggttgctgtt tttgatcaac cagggttctt actgcccggc caaatgaatc
      961 aaatactgta atcctgacat cacccggttc attcagggta tattcaatgt taagaaattt
     1021 ttccgacggg ttaggataga tcctgaaatc cggatcatta attatgtttt catgaacgga
     1081 ggtggcatag gcatgcatca gcaggggata ctgcgcggta ccaagtcgca tatcgtcggt
     1141 aaatatcacg gtttcactac agttatagat tttctttgta tctgaattat aatatctgaa
     1201 agtaactgtt tcaccggtta caagattact atatatcatc agactgaaat ggtacgtgcc
     1261 gtccaaaaat tccatgccgg ctgtaaggcc tcttaattca tcacccacat aggcatacaa
     1321 tgtatcattt tccgaacctt tgggaatatc atccaatata actctcacaa aaacttcgcc
     1381 gctaaattca tatgggtggg gattgaatgc accggtctgt tccgttcctg aagacatctg
     1441 atttcctttt ccttccggtt caggataaac aagcgttccc ggatttgtaa ctctcagcat
     1501 gtatcccctt tggggttcca tgtcatccat agtaccatac cacaggctaa agtctgcata
     1561 gtaagttgaa gaacttacct gatccttgat ataatccaga tctgaaggac taagggattg
     1621 cagggctgtg ttcacaggca tggcctgacg cggaagatat ccaatccagt tccagccgct
     1681 ggcaagagga atattgattg cattaatatc aacaggcctt ccgctgaaat gaatcacact
     1741 gttattctgc agccttatct tatataattc tctggaatca agccggtcaa gcgtgccaaa
     1801 ccaacccagt ccctcataat atgtagctga tactgtctgg tttttaatat aatcacccgg
     1861 agaattcgct gcctccagta taaactgagg tgtcatattg tcaagggttg tattgaaaga
     1921 aaaccagttc catccggctg aaagggagac agagtaatcc gtaccgatat tcatgggaaa
     1981 tggtgccgaa gccgatcctg tattcatccc tgaagaaaag tcaactgatc tgtcaagatt
     2041 atattccatg actactgcag gattgaaata tctgaatacc aacacatctc ccgtttcagt
     2101 attgctaaaa cagttaagtt caaagatata attaccggac tgaggattgt atactgcctc
     2161 tgttattccc ctgcactcgt ttccggcaaa tgctgcaaga aatcccgatt cagctcctgt
     2221 tccttcaatg gtaactgagg aagttatacg gccctgatga gtaaaattct cagaatcaaa
     2281 ctcccagccc gaaggaagaa cattgaatgt cattgaaact gcacagccgg tgggcagagt
     2341 gtatgtaatt agggcagttc ctgcagtcat cccccttact tccccgctgg aagggtttat
     2401 ttcagcaacg gaaggaacac tgctgctcca ggaaccaccg ggtgaagtat tggataaagt
     2461 tgttgatgat cctgctgcgg tgtaattata tccggtaata gcagaaggta aaggattgat
     2521 taaaacactg gtggttccgg cattactggt acagcctgca actgttactg taacagaata
     2581 agtacccgat gctgcagttg tgacattcgc aatgctggga ttctgctggg tagatgtaaa
     2641 tccgtcggga cctgtccagt taaagatacc gccggttaca tccgaagtag taaggttcag
     2701 tgtgccgccg gcacaaagtg gactgttgtt tgagggtacg ggtgtagcag gaatagcatt
     2761 tatgactaca gtggtggttc ctggttcact cgtacagccg tcaactgtta cagctacaga
     2821 ataagtaccc gatgctgcgg ttgtgacatt gggaatgacg ggattctgcn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt acagaataag tacccgatgc tgcggttgtg
     3001 acattgggaa tgacgggatt ctgcagagtg gatgtaaatc cattggggcc tgtccagtaa
     3061 aaggagccgc cgggtacatc cgaagctgca agaagaaggg tttctccttc acatatgggg
     3121 ctgttgttct tcacatcagg cggctgagga attgcattta ccctgacttc agtggttccg
     3181 gcattactgg tacagccggc aactgttact gtaacagaat aagtacccga tgctgcagtt
     3241 gtgacattcg caatgctggg attctgctgg gtagatgtaa atccgtcggg gccggtccag
     3301 ttatatattc cttctatttc tgtggaagtg aaaagattca gtgtttcccc ttcacagaga
     3361 ggtccgttat tcgatgccga aggtgtatca ggaacagtat tgacatgcaa taaaaaaact
     3421 gatgtaagca cttcacactc ctgatcagga ttatttgctg tcacggtaat ggttacagta
     3481 gaacccgcat ccgctacggc agggttgtat gtaaatgaaa aaggtgaaac atcaattatt
     3541 tcctgatcca aagtaccaga accattcgtg cttatagtaa cgcttattgc accgccaccg
     3601 aattcattat tttccaccgt cagcggatgt gtcccgcagg tgctgccgga tgccgcgcct
     3661 agtaccagaa cggatggatt atcacactcc tgtccgtaga gttcagcatt gagaaataaa
     3721 aacatactcc ccagaaacat tgccaaacaa aatttctgta atattctgac agaactgcct
     3781 gtgttgcggt agaattgttt catggtatga tgtttacggt taatgataga ggatttaagg
     3841 aagtaaaaac aagagaaccg gatataatcc tggattctta atcctatttt ccggatccgg
     3901 acatgctatc gccccgtcag tcacaccgga atgtgccgac aagagcatgg ccaacactgc
     3961 ctgaggcctg cagagcttta cataaaatca ggatattcaa atgataaaaa tatctttcct
     4021 ttcagattcc ggaaaggtcg atgaaacgta tgtaatgccc agcctctttt taaaatcaac
     4081 atctaattta tagtatttga aggagaaatg taattttatt tgaccaagag aagaaaatat
     4141 tggatgaact ggttttttgc gtaaatgaca cagttatggg gcttcggctg caaggctgat
     4201 gaacacaggt ctgatccgga ataccggatg gaggaatatg acaaagtatt gaagtttttt
     4261 tcttttcttt aaaacttgtt tgatagtttt tcctaatatt gaccatgtaa tgccgtcctg
     4321 ccggaatcca ggaaaaaata ctggccggca gctaaaatca ggtcaaaccg tacaccttgg
     4381 aaacacactg tatttgcata ttatttccgg aagaaaaact ttgtaagtcc ttaattttta
     4441 aaattcaata ctgatgaaga aaaataaatc cataactgtt ctggctgtta tagccgcatt
     4501 cattattgca gcgggaagca acacttacgg acaatcgaaa aaagaaacac ctttcaatat
     4561 cattacctac aacatcaggc ttaacacttc atcggatggt gaaaatgcct ggccgttccg
     4621 taaggacagg gtggccggac ttctgaaatt tcacagggcc gatattttca atgtacagga
     4681 agctttgccg gaacagatgg atgatcttgt tgctgctttc ccggatttcg gtcatgtagg
     4741 agtcggacgc gatgacggaa aaagagaggg ggaacacatg tcagtctttt acagcaaaaa
     4801 gagattcacc aaacttgatg atggtatgtt ctggcttagc gaaacgcccg agaaaccggg
     4861 actgggctgg gatgccgcct gcaaccgtac atgtacctgg ataaaactga aggataatat
     4921 taccgggaaa aaattcctcg tctttaacac gcatctcgac catcggggaa acaaggcaag
     4981 gcaggagggc gtccggctga ttcttgaaag aatggccctg ataaacaagg aaaacctggc
     5041 agtgattttt acaggcgatt tcaatcttgc aaaaacagcg gcacccattc agtctgtcct
     5101 gaaagtgctc gatgatgcca gggacaaatc acttacacag cactacggac cggacggaac
     5161 ctttacaggc ttccctgtaa aagccaatct gacgggagcc attgacttta ttttcataaa
     5221 caataaagtt aaggtacttc gccacgggac actgaccgat tccttcaacc tctatcaccc
     5281 ttccgatcat cttccggtgc tagctgaggt acagatcgac tgatttcacg aaggaatccc
     5341 ctgtcctgct ccgggcggga gattaaaacc gcggttcagc agtgttcagc tctttatcct
     5401 gtccataatt tcattgaaaa gtgtcagctg ctccctcatg acagctacgg gatcagtata
     5461 gggtgtagtg gcctcgacaa gtatccaccc cctgtaattt attccgtgaa gaagcctgaa
     5521 cagttcctca tagggataat cactgatctt tattcctctt atatgagttg tatcaccgat
     5581 ccattgcctg accgaattga aattggcttc cagtccggga ggattcagat caacatcatt
     5641 gctgttccag cacattttaa cattcttctc ggtcacctga tcaaaaatcg ctttcatgtt
     5701 cggtatttcc tgggtaacgg gcccgtggca ttcaaccctg atgatctggc cataatccct
     5761 ggcaaattta cccagttcat tcagggacct ggcaatctgg gctattgtct tttccctctc
     5821 cacttctttt ggaagtgtat ttggcttcac cttcagcccg gtggcaccaa tatccttaca
     5881 aagctttaca tattcctttg ccccttctat attttcacgg agcctggcag gatcaggact
     5941 gtgaaactca taatttgacc cgtaaccaac gcatgtcacg ggagagtctg caaaacgttt
     6001 ctttacttcc ttgcgctggg caggagtgag atcaatctcc accttgtgtg catgctgtgt
     6061 acggagctcg actccgagaa gacctgactt ctcacagttg gaaatcaggg tcgggaggtc
     6121 ccatttggct ccccacatat atgtaaccag tcccatctga agacctttag gtctttttct
     6181 gtccattgcc gcagcgggaa gagcattaag ggcagccaga gccacacctg cacccagggt
     6241 tctctgaata aactgacggc gtgataattc gtttttcatg gtttgtgttt tatttcatta
     6301 tacctatcaa ttgcatatgt attcttctgt catgtttcat gctccacgtt ccggaccttg
     6361 ccgcagatat ccctgagccg gctttccatg tttttctttc cgcatcagtt atttatatct
     6421 gccagcaaat tcatctgcca gtttgttgat ttccgcatct gtcagatgta ttcccgaact
     6481 gattataacc ctgtatctga aagtgatttc ctccccgggc cggatggaaa aatccagcct
     6541 ttcccttccg cctgtgaaat cagcccatcc cagaggattt gcggcaaaaa gaccataccc
     6601 gcgggcatgc cagtaagtgg gatagccggg attatccgga tgatcacaga caaccagcga
     6661 gatcttttct tcaccgatat tgccgtacag attcatccat ttcgcacgtg tcccccagac
     6721 atcgtcgccg ctttttccct cgctgctcct gtaattgccg gtagcgacat cccctgccct
     6781 gactttctcc ggcaaaggct ttccgtccgg tccgagaaca gtcaccatat cgtcatatgg
     6841 cagctccagc tgacgggcaa cccttatccc gaacattcct tcctttgtat cgtcaaaatt
     6901 tacagcaaat gaaccggctt tcaaagttgt cagcctgtca attattctga gagattcctc
     6961 tgcgagaaaa tgatattccg tattctccgc aagaagctca acaccgtcag gatcaaccca
     7021 gctttccttt gtaagaagaa tggcctcccc gtttttttct tccatttttt caatggaaag
     7081 atgctttatt tcaccgccgc ccggctcctt catccccctg tggccgttac cccagaaatc
     7141 aagaccgtta accttcccgt aattcagcca tattccgacc tggtgaatgt ggtcattccg
     7201 ctccccttcc ctgggaacaa gcggaaaacc cctggtaacc tccgttcctg cagccgtttt
     7261 aacaggataa aggatgggtt tataaacatt ttctggccag cagaaggagg tgaaatgatc
     7321 cttgccaatg aatacatcca ctttccgttc tgtctcattc cttacgaaaa atatctttga
     7381 actgtcgttc cgtgcagacc ttttcgtatt gcagctcagg gaaacgagaa gaattaaaac
     7441 aggcatcgaa gctcttactg ctgtattttt catttttccg gtttttatta tgttaattta
     7501 tacttagtta acaaatttgg tttcgtgttc tgtcatccat cactgtaaac agtacaaatc
     7561 agggaaaagt acagcaggag gacagattcc gaaataaaat gtataaaact ttccgaaaat
     7621 attcggaaaa gcacatggct tatcaatccg gtctcaaagc ccctgaactg ctgcctgtat
     7681 ttactgtctg gaacagctgc cggctggtac tgactgcagc ggcagatccg gtaaaaccgg
     7741 atgatcagac aactgccgga acaacgtagg gtttcctgta ttcccttgtc agcattttat
     7801 cagcttcctt atccttcacg aactgctcct tggttccgtc gaatgtaagt tctcttccaa
     7861 ggcgatagga aatatttgca aggctgggca ggcaggagga catatagcct tcaaggatgt
     7921 cgcagtgaag cgggccatta ttccctgcac ggatagcctc aaggaagttt acatagtggc
     7981 ttccgccgcc cggcgccttc atcagatcga caggcctttc ccctatatcc gatcctgcaa
     8041 agggttgcct ttcacgctga cggtaggctt tccactcgct tccgtttatc tcgacgtatc
     8101 cctccgttcc cagaaacatg ttgcctatct gtatgccaag gcctgattcg gcatttgtaa
     8161 aacggcccct ggtttcaaat tccagaattg tcccatcggc atatttaaat accgaagcct
     8221 gtgtattggg agtctcctgt gcacattcct tcggatcaat ccc
//
LOCUS       DAOSPX010000125         8261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8196_length_8261_cov_4.334877, whole genome shotgun sequence.
ACCESSION   DAOSPX010000125 DAOSPX010000000
VERSION     DAOSPX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8196_length_8261_cov_4.334877"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /gene="rpsB"
                     /locus_tag="PLZ75_07445"
     CDS             <1..921
                     /gene="rpsB"
                     /locus_tag="PLZ75_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOS72265.1"
                     /translation="FGHLKRKWNPAMAPYIFMERNGIHIIDLEKTIDKLDEAASAMKQ
                     IAKSGKKILFVATKKQAKEIVADKVNRVNMPYVTERWPGGMLTNFPTIRKAVKKMSSI
                     DRMAADGTFSNLSKREKLQITRQRAKLEKNLGSIADLTRLPSALFVVDICKEHIAVRE
                     AKRLGIPVFAMVDTNSDPTEIDFPIPANDDASKSISLITEILCQAIEEGLNERKMEKD
                     KEPGTRERRVVRKDTVAAGVSASLSGLDGDDEEEEEESVPRRDYSRPDEDADAEDTDE
                     DDDGEDSDDDDDSGDTDEDDDLIYSIDEEE"
     gene            959..1789
                     /gene="tsf"
                     /locus_tag="PLZ75_07450"
     CDS             959..1789
                     /gene="tsf"
                     /locus_tag="PLZ75_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOS72266.1"
                     /translation="MATISAADIARLRRMTLAGMMDCKKALEEAEGNFEKAVEIIRKK
                     GQSVANKRADREASEGVVLARVSDDGKRGIMIVLNSESDFVAKNAEFVGFANQILDLA
                     MKSNPADLEELKSLPMDGVTVGEKVVEYIGIIGEKLELSAYYRIEAPFVQAYIHPGNK
                     LAALTGFDRAGLDQQVYKDVVMQVAAMNPVAVDKGDVPQSVIDQELEIGREQARREGK
                     PEAMLEKIAQGKLAKFYKESTLLNQDFIKDSKITVGQYLKTAGDSLKVTGFERHSLTI
                     "
     gene            2102..2812
                     /gene="pyrH"
                     /locus_tag="PLZ75_07455"
     CDS             2102..2812
                     /gene="pyrH"
                     /locus_tag="PLZ75_07455"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOS72267.1"
                     /translation="MKYRRILLKLSGESLAGSGSYGIDGSVLDFYAKEVAELAEKKYE
                     ISIVIGGGNIFRGLSGSESGFDRVKGDQMGMLATVINSLALENAIQNKGYAAKVYTSI
                     RMEPVGNLYIRDTVNEELKKGTVCIIAGGTGNPFFTTDTAAALRAVELKADLLLKGTR
                     VDGVYTADPEKVADAEKFDSVSFDEVISRRLKVMDSTAFTMCRENRMPVIVFNMNIPG
                     NLKKVVSGQKTGTLIYPS"
     gene            2914..3477
                     /gene="frr"
                     /locus_tag="PLZ75_07460"
     CDS             2914..3477
                     /gene="frr"
                     /locus_tag="PLZ75_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOS72268.1"
                     /translation="MNEDVELIIEETRDRMQKAADHLEHELARLRAGRANPVLLEGIT
                     VVYYGVNSPLSQVSNINTPDPKTILIQPWERSMLGAIEKAILAANIGLTPVNNGEVIR
                     INIPPLTEERRHQLVKQVKNEGETARISIRNARKWANDELKSLLKSGLPEDTEKTASE
                     TVQKITSEFNATIDRIVAQKEKDVMTV"
     gene            complement(3464..5854)
                     /locus_tag="PLZ75_07465"
     CDS             complement(3464..5854)
                     /locus_tag="PLZ75_07465"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOS72269.1"
                     /translation="MKILKTILLVLAAILAIAIIGGLILLSGIKKGALPVYSGELKIS
                     GLNGEVTVYRDERGMPHIYAQDEHDLYFATGYVMAQERLWQMDLIRRATKGKLAELFG
                     DDFIQTDLFLRSLDMTTKSKMVLSNQPQEITLCLQAFAGGVNSYISDAGRKLPPEFRI
                     LSYRPDEWQLEDIANIIGYMGWDLASGNLTTDIFIYRLIEKLGEEKVSQLVPDWGVES
                     SVVFPEFRLEDDMLRDARAFIKTLDQLEALGIHSFSGSNNWAVSGSRSATGMPLFSND
                     MHLGLSSPGIWMQMHQVVPGKLNVTGVIVPGEPFIVAGHNENIAWGLTNLMVDDVDLF
                     REEINPDNENQYYFNGEWRNMLVRKEIIRSKTGRTDTMLLRFTHRGPVISGMQKVENA
                     VLTMRWSGYDPSDELKAVYSINRAENWEDFRKGVAHFSSISQNFAYADREGNIGIVTG
                     GGIPVRKGYGSMIRPGQTDEFDWKGYVATEQLPYSFNPESGYVSSANNKTVSDEYPWY
                     ISFRFYVPYRIDRIREMITDREKLDVNDFKRMLTDQHSAYAKLLTPFILRVSDKRPEL
                     NTTENQVLDIMTGWDYDMRADEAAPAVFEFFSHSFAENLLGDELGELYGQLPDAIKDY
                     YIYRILKNGPDGWVDDVRTTDTETLDDIILRSFRDATGELSESLGNDVSKWKWGDIHR
                     ITLEHPIGTVKIMDRIFGLNSETYPVGGSDHTVCPYTYNDLFRVTNGASERHIYNLAD
                     WDESWTVIPTGASGVPASEFYLSQTKTYVEGGFYRDLFSEAAVRAAARYVLTLKPS"
     gene            complement(5891..>8261)
                     /locus_tag="PLZ75_07470"
     CDS             complement(5891..>8261)
                     /locus_tag="PLZ75_07470"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOS72270.1"
                     /translation="CGKACSKVDYCYEGVLTVSGLSDTVTIYRDERGMPHIYASNEHD
                     LYLAVGYVSAQERLWQMDLIRRSTTGRLSEIFGKSFLQADIISRCLRMTDKSGKILEN
                     EDPSVIACMQAYIEGINFFILNCKKLPLEFRLLSYRPEPWSLEDIVNIIGLMGWSLGS
                     RNLTAELFNYQLVRKTGAEKASSLIPDWDLATEIVYPDFVLNDTLISEVRSLITSFDR
                     IISLGVPLFSGSNNWAVSGERSATGKPLLSNDMHLPFNIPSIWIQMHHIVPGKINVTG
                     VVIPGEPFIVAGHNDKIAWGMTNLMVDDIDLYTERINPENHDQYYFNGQWKDLIKKTE
                     IINIRGGKQIPVTIGFTHRGPVISGLINPDNMPAKIKWMGYDYLAGLEDLKDLSLSFR
                     WSGFDISDEIKGIYLLNRAQNWEDFRSGVALFNSTSHNFIYSDIYGNIGLNAGGGIPL
                     RKGNGIMIRNGETDEYDWRGYVPFDQMPFSFNPPDGNVSSANNKTVAGYPYFISQDFV
                     VPYRIERIREMLGQKELFSTEDFKRMITDQQSVMARLLTPFILKLKEKRNELSQIENA
                     ILDSLSAWDFIMDPSRIAPSVFEFFRICLKKNLFADDLGDLFNEMYYMTSEYYIYRLI
                     TGEADEWIDNLNTPEKETLDDIVMESFKDAIEMLSKQYGKNPARWQWGRIHTITFIHP
                     LGSVKILGSLFKLNSGEYPTGGSDHTVCPFSFKAGFRVSQGASIRHIFNTADWDQSYS
                     VLPGGISGVPKSEFYLSQVEPYLEGRFYNDHFSEKAVKNSAKHRFLLRP"
BASE COUNT         2294 a         1961 c         1876 g         2130 t
ORIGIN      
        1 ttcggacatc tgaaaagaaa atggaatcct gccatggctc cgtatatttt tatggaacgc
       61 aatggtattc atatcattga tcttgaaaaa accattgaca agcttgatga agcagcttca
      121 gcaatgaagc agattgccaa gtcgggaaag aaaatccttt tcgtggcaac gaagaaacag
      181 gccaaggaga tcgttgccga taaagtcaac agagtaaata tgccctatgt gaccgaacgc
      241 tggcccggcg gcatgcttac gaacttcccg accatccgca aagcggtcaa gaaaatgagt
      301 tccattgaca gaatggctgc cgacggcaca ttctcaaacc tttcaaaacg cgaaaagctc
      361 cagatcacca ggcagagagc caagcttgaa aagaacctcg gaagcattgc cgaccttaca
      421 cgccttccct ctgcactttt tgtcgtcgac atatgcaagg aacatattgc cgtgagagag
      481 gcaaagcgcc tgggtatacc ggtatttgca atggtggata caaactccga tcccacggaa
      541 atagattttc cgatacctgc caatgacgat gcctccaaat cgatttccct gataactgaa
      601 atactttgcc aggctattga agagggtctc aatgagagaa agatggagaa ggacaaggag
      661 ccgggaacca gggaaagaag ggttgtcagg aaggatactg ttgcagcggg agtatccgcg
      721 tcactctcag gcctggacgg cgatgatgag gaagaggagg aggaaagcgt accgcgcagg
      781 gattattccc gtcccgatga ggatgcagac gctgaagata cggatgagga tgatgacggg
      841 gaagacagcg acgatgatga tgattcaggc gacaccgacg aagatgatga cctcatctac
      901 agtattgacg aagaggaata aacaatataa ttaatcaata aatttaaaac caggatctat
      961 ggctacaatt agtgcggcag atattgccag actgagacga atgacccttg cagggatgat
     1021 ggattgtaag aaggctcttg aggaagccga aggcaatttt gaaaaggcag tggaaataat
     1081 caggaaaaag ggacagtctg tagcaaacaa gagggctgac cgggaagctt ctgaaggagt
     1141 tgtcctggcc agggtaagcg atgacgggaa aagaggtatc atgatagtgc tgaacagtga
     1201 atcggatttc gttgccaaga atgccgagtt tgtgggattc gccaatcaga tactggatct
     1261 tgccatgaaa agcaatcctg ctgatctcga agaacttaaa tccctgccca tggatggtgt
     1321 cactgttggt gagaaagtgg ttgagtatat aggaattatc ggtgagaagc tggaactttc
     1381 agcttattac aggattgaag ctccctttgt acaggcatat attcacccgg gcaacaaact
     1441 ggccgccctc acgggttttg acagggccgg actcgaccag caggtctata aggatgttgt
     1501 aatgcaggtt gccgcaatga atcccgttgc tgttgacaag ggagatgttc cacagtcagt
     1561 tatcgaccag gaactggaaa ttggaaggga acaggcaaga cgcgaaggca agccggaagc
     1621 catgcttgag aaaatagccc agggcaagct tgccaaattc tacaaggaaa gcacactgct
     1681 gaaccaggat ttcattaagg acagcaaaat aaccgttggc cagtatctta aaacggcagg
     1741 cgactcgctt aaggttacag gctttgaaag acacagcctg actatttaaa tcctttcact
     1801 gccggcacct ctcatcagaa caggaaaagg gaccgccccg ggtaacgcag gaagcctgca
     1861 atgaacgatc cggggtaacg caggaaacct gcaatgaacg gtcccgctca acggaggaag
     1921 cctgcaatga acggtcccgg gcaacggagg ataccgaacc ggacggaacc agtgctgaag
     1981 acatatttcc ccgtaccgta acaggtgtca ccaaaggggt aaaacaatca gggagacatt
     2041 cattgtctcc tttttttatt aatttcgcgc tgtttcatca ctgttagtcc cttacatgaa
     2101 tatgaaatac aggagaattt tattgaaact cagcggtgag tcccttgccg gatcaggcag
     2161 ttacgggatt gacgggtcag tacttgattt ctatgcaaaa gaagtcgcgg aactggctga
     2221 aaaaaaatac gaaatatcca tcgttatcgg aggcggcaac atcttcaggg gcctgagcgg
     2281 aagcgaatcg ggatttgaca gggtaaaggg cgatcagatg ggtatgcttg caacggtgat
     2341 aaacagcctg gcactcgaaa acgcgataca aaataaggga tatgccgcaa aggtctatac
     2401 ctcgataagg atggagcctg tcggcaacct ctacatacgt gatactgtaa acgaggagct
     2461 gaaaaaaggc actgtctgca ttattgccgg cggaacgggc aatccgtttt tcactaccga
     2521 taccgctgcg gctctgcggg ctgttgaact gaaggccgat cttcttctga aaggcacccg
     2581 ggtggacggg gtttatacag ccgatcccga gaaagtggcc gatgccgaaa aattcgacag
     2641 tgtttcattt gacgaagtca tctcccgccg gctgaaggtc atggattcga cagcctttac
     2701 catgtgccgt gaaaacagga tgcctgtcat tgttttcaac atgaatattc cgggaaacct
     2761 gaaaaaggtt gtcagcggcc agaagacggg aactctcatt tatccctcct gactgatcac
     2821 acttttatct atattttatt aaatttgtca taatacggcc gggaaagcgg ccttttacac
     2881 aacaggaaat tattatctaa caattctgac aatatgaatg aagatgttga actgattata
     2941 gaagaaacaa gggacagaat gcagaaagcc gctgaccatc ttgagcatga actggcaagg
     3001 ctcagggcag gcagggccaa tcctgttctc cttgaaggaa ttacggttgt ttactatgga
     3061 gtcaattcac cattgagcca ggtttcaaat atcaataccc ccgatcccaa gacaatactt
     3121 atccagccct gggaaagaag catgctggga gccatcgaaa aagccatact ggctgccaac
     3181 attggcctta caccggtcaa taacggggaa gttatcagga tcaatattcc tccccttact
     3241 gaagagagac ggcatcagct ggtaaaacag gtgaaaaacg agggtgaaac agccagaata
     3301 agtatcagga atgcacggaa gtgggcaaat gatgagctta aaagcctcct aaagtcagga
     3361 cttcccgaag acactgaaaa gacagcatcg gaaacggtac agaaaataac ttcggaattc
     3421 aatgccacta tcgacaggat cgtggcacag aaagaaaagg atgttatgac ggtttaagcg
     3481 tcagtacata cctggcagcc gccctgaccg cggcctcact gaagaggtcc ctgtaaaatc
     3541 ccccctcaac ataggtcttt gtctgtgaaa gatagaactc actggcagga acgcctgatg
     3601 cacccgtcgg aatgaccgtc caggactcat cccagtcggc aaggttgtag atatgccttt
     3661 ccgaggctcc gttcgtaacc ctgaaaaggt cgttgtaggt gtagggacaa acagtgtgat
     3721 cactgcctcc cacagggtat gtttcggaat tcagcccgaa aattcggtcc attatcttca
     3781 ctgtccctat aggatgttcc agtgttatcc tgtggatatc cccccatttc cattttgaaa
     3841 catcattccc cagggattct gaaagctctc ccgtggcatc cctgaagctt ctgagaataa
     3901 tatcatccag ggtctcagtg tctgtggtcc tgacatcatc cacccatccg tcaggtccgt
     3961 ttttaagtat cctgtagata taataatcct ttattgcatc aggcagctgg ccatagagct
     4021 cacccagttc gtcacccagg aggttttccg caaagctgtg ggagaaaaac tcaaagacag
     4081 ccggagctgc ctcatctgcc ctcatgtcgt aatcccagcc ggtcattatg tcaagcacct
     4141 ggttttcagt tgtgttcagt tctggcctct tgtcactgac tctgagaata aagggagtaa
     4201 gcagttttgc ataggcagaa tgctgatcag tgagcattct tttaaaatca ttcacatcaa
     4261 gcttttccct gtccgtgatc atttcccgta tcctgtcgat tctgtaggga acatagaatc
     4321 tgaatgaaat ataccaggga tattcatcgc ttacggtctt gttattggcc gatgacacgt
     4381 aaccggattc cggattgaag ctgtacggaa gctgttccgt ggcaacataa cccttccagt
     4441 caaattcatc cgtctgcccc ggtctgatca ttgaaccata gcccttcctg acgggtatgc
     4501 cgccaccggt gacaatccct atgttgcctt ccctgtcggc atatgcaaag ttctggctga
     4561 tggaactgaa atgtgccact ccctttctga agtcttccca gttctccgcc ctgttaatgg
     4621 aataaaccgc cttcagttca tcactgggat catagcccga ccaccgcatg gtcagaacag
     4681 cattctcaac cttctgcatg ccggatataa ccggtcccct gtgggtgaat ctcagcagca
     4741 tggtatcggt cctgcctgtt ttcgatctga tgatctcctt tctcacaagc atgttccgcc
     4801 attcaccgtt gaagtaatac tgattctcat tgtccggatt tatttcctcc ctgaaaagat
     4861 caacatcgtc aaccatgagg tttgtcagtc cccatgctat attttcattg tgcccggcaa
     4921 cgatgaaggg ttcacccgga acaatcactc ctgtcacgtt cagcttaccg ggaacaacct
     4981 ggtgcatctg catccatatt cccggagacg acagccccag gtgcatgtca tttgaaaaga
     5041 gaggcattcc cgtggcgctc ctgcttcccg acacagccca gttattgctt cccgaaaagg
     5101 aatgtattcc aagcgcctca agctgatcga gtgtctttat gaaggctctg gcgtcccgga
     5161 gcatatcatc ctcaagcctg aattccggga ataccactga agactccacg ccccagtccg
     5221 gaaccagctg cgagaccttc tcctctccca gtttttctat aagcctgtat atgaatatgt
     5281 cggtagtaag gtttccggat gcaagatccc agcccatata gcctataata tttgcaatat
     5341 cctcaagctg ccattcgtca ggccggtatg acaagatcct gaattccggc ggcagcttcc
     5401 ttcctgcatc actgatatag ctgttcaccc cgccggcaaa ggcctgcagg cagagtgtta
     5461 tttcctgagg ctggttgctg agtaccattt tcgactttgt ggtcatgtcg agactgcgga
     5521 gaaaaagatc ggtctgaata aaatcatctc caaacagttc agccagtttc ccctttgtgg
     5581 cacgacggat gagatccatc tgccagagcc tttcctgtgc cattacataa ccggtggcaa
     5641 aatagagatc atgctcatcc tgtgcataaa tatgaggcat tcccctttca tccctgtaaa
     5701 cagtcacttc cccgttcagt ccgctgattt tcagttcgcc gctgtaaaca ggcagggcgc
     5761 ctttctttat tcctgaaagc agtatcagtc cgccgataat agcaatagca agtattgccg
     5821 ccagtacaag taaaatggtt ttaaggattt tcatggtttt gctgattatt tatctgattg
     5881 gtaattattt ttacggcctt aataaaaatc tgtgttttgc tgaatttttt acggctttct
     5941 ccgagaaatg atcattgtag aatctccctt caagataagg ttcaacctgt gaaagataga
     6001 attcgctttt tggtacaccg gatatccctc ccggaagaac cgaatatgat tgatcccagt
     6061 cggccgtatt gaatatatgt cttatagaag ctccctgaga taccctgaat cctgctttaa
     6121 aggaaaatgg acaaaccgtg tggtcgcttc cgcctgtcgg atattcccct gaattaagtt
     6181 tgaacagtga tccaaggatt ttaacggatc cgagcgggtg aataaaagta atggtatgga
     6241 tccttcccca ttgccatctg gcaggatttt taccatattg ttttgaaagc atttctattg
     6301 cgtccttaaa actctccatc actatatcat caagtgtttc tttctccggt gtgtttaaat
     6361 tatcaatcca ttcatccgct tctcctgtaa taagtctgta aatatagtat tcactcgtca
     6421 tatagtacat ctcattgaaa agatcgccca gatcatcagc aaaaagattc tttttaagac
     6481 agatcctgaa gaattcaaat acggagggag cgatccgtga ggggtccatt atgaaatccc
     6541 acgctgacag agaatcaagg atggcgtttt ctatttgtga aagctcgttc cgtttttcct
     6601 taagttttaa aataaaagga gtaagtaacc gggccataac cgattgctgg tcggttatca
     6661 ttcttttaaa atcttctgtg ctgaacaatt ctttttgacc gagcatctcc ctgatccttt
     6721 caattctgta aggaacgaca aaatcctgtg aaataaaata tggataaccg gccactgttt
     6781 tattatttgc ggatgataca tttccgtccg gaggattgaa gctgaaaggc atctggtcaa
     6841 aaggcacata ccccctccag tcgtattcat cggtttctcc gtttcggatc ataattccat
     6901 tccctttccg caacggaatt ccgcctccgg cattcaaacc gatgttcccg tatatatccg
     6961 agtatataaa attatgactt gtggagttga ataatgccac tcccgacctg aaatcttccc
     7021 agttttgagc cctgttaaga aggtaaatac ctttaatttc atcacttata tcaaatcccg
     7081 accatctgaa gctcagtgaa agatctttaa gatcttcaag cccggctaaa taatcatacc
     7141 ccatccattt aatctttgca ggcatgttat ccggatttat caatcctgat ataaccggtc
     7201 cccggtgtgt aaaaccgatt gtcaccggaa tctgtttacc tcccctgata tttattatct
     7261 cggttttctt tatcagatct ttccactggc cgttaaaata atactgatcg tgattttccg
     7321 gattgattct ttcagtgtaa aggtcaatat catccaccat caggtttgtc attccccagg
     7381 cgattttatc attatgcccc gccacaataa aaggttcccc ggggataacc acgcctgtga
     7441 catttatctt accgggaact atatggtgca tttgtatcca tattgacgga atgttaaacg
     7501 gaagatgcat atcgtttgaa agcagaggct tcccggttgc acttctctct cctgagactg
     7561 cccagttatt gcttcctgaa aataagggca ccccgagtga aatgatcctg tcaaatgatg
     7621 ttatcagtga ccgtacctct gagatcaggg tgtcgttcag cacaaaatcg ggatagacaa
     7681 tttccgtagc cagatcccag tccggaataa gagatgaagc cttttcagcc cctgttttac
     7741 gtacaagctg ataattaaaa agttctgccg tcaggtttct tgagccaagg ctccagccca
     7801 tcaacccgat tatattaact atatcttcaa gactccaggg ttcaggtctg tatgataata
     7861 acctgaactc cagcggcagt ttcttacagt ttaaaatgaa gaaattaatc ccttcaatat
     7921 atgcctgcat acaggcaata actgaaggat cttcattttc caggattttt ccggatttat
     7981 ctgtcatccg gaggcatctc gatattatat cggcctgcag aaacgatttc ccgaagatct
     8041 cagataacct ccccgttgtg ctgcgccgga tcagatccat ctgccagagc cgttcctgtg
     8101 cggatacata cccgaccgcc agataaagat catgttcatt tgaagcataa atatgtggca
     8161 tgccacgttc atccctgtaa atggtaacag tatctgaaag accgctgacc gtgagcactc
     8221 cctcataaca gtaatctacc tttgaacaag ccttgccgca g
//
LOCUS       DAOSPX010000126         8237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8227_length_8237_cov_4.535810, whole genome shotgun sequence.
ACCESSION   DAOSPX010000126 DAOSPX010000000
VERSION     DAOSPX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8227_length_8237_cov_4.535810"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLZ75_07475"
     CDS             complement(<1..251)
                     /locus_tag="PLZ75_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS72271.1"
                     /translation="MKDIPVIYVRNLVKKFGSFTANDGLDFEVYEGEIFGFLGANGAG
                     KTTALRILSGLSKPTSGIVRVSGFDAGNEAEQIKRNIGY"
     gene            complement(325..1236)
                     /locus_tag="PLZ75_07480"
     CDS             complement(325..1236)
                     /locus_tag="PLZ75_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS72272.1"
                     /translation="MEKIIEAQGIGKSFGDTEALKNISFEVNRGEIFGFIGPDGAGKT
                     TLFRIITTLLLPDAGRMTVMGLDCKTGYRELRRNIGYMPGRFSLYHDLTVEENLNFYA
                     TVFGTTVRKNYDLIRDVYSHIEPFRKRLAGKLSGGMKQKLALSCALIHKPGLLVLDEP
                     TTGVDAVSRSEFWEMLNRLREENITIIASTPYMDEAMRCNRVALIQKGQILSVNPPEK
                     IREEFSRKIFSVKSSEKYKLINALRNFPGTATAYPFGDSVHLTFRDNEQDHSLTGYLN
                     SMGISDATISETRAGIEDRFLELMQDK"
     gene            complement(1242..2120)
                     /locus_tag="PLZ75_07485"
     CDS             complement(1242..2120)
                     /locus_tag="PLZ75_07485"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOS72273.1"
                     /translation="MRNISLVIITASMLAACSGKSGEADAYGNFEATEVIVSSETSGR
                     ILRFEAREGSEIEKGDEIALVDTMLFHLQKAEIDAAMRSVSTRIASINAQNDIIGQQI
                     ANLRVNIARIENMLKNDAATQKQYDDLAGQVEVLQKQAAANNTQKASVAAELTVFETK
                     KATLAEQLARSCVRSPIGGTLIEKYSEAGEMTTAGRPLVKIADLSLLKLKVYVSGGQL
                     GSLRVGSECTVRIDEGEKGYRSFPGRISHISEKAEFTPRIIQTKEERVTLVYAVSIEV
                     RNDGSMKAGMPGEAIF"
     gene            complement(2200..3513)
                     /locus_tag="PLZ75_07490"
     CDS             complement(2200..3513)
                     /locus_tag="PLZ75_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS72274.1"
                     /translation="MKQYTDMQKLYFLLFIVLTGIMPLKAQKVITLKECYEMAYATAP
                     LSAEKEVYDNIWQIRNRNLLKGWLPSLDANGSFIYNSSVVNMEDAIGSLPLPGTGGLL
                     KPLPHEQYRITLDISQMIYDGGAIKSARALEEADLHISKKQNESDLYKLRAQVNSHYF
                     SMMLLDRQKELLQNYLEVIDRRIASLNSAVLNGMILKSDIDVLSSERLKLEQQLTENG
                     IRKKSLLRILSALTGTEIDTSVRFVLPPVKEELTDELARPELRIFDLRREQLDASLSL
                     INSERMPRAFGFATFGYGNPPGNNFFRDEFAPYYILGAGIRWNIFDWNKARNEKQIVS
                     LRQNLIQARKNDMEDNLKRLLDAKSAEISSLESLLLSDTELIGLRKRITAAAGSQYDN
                     GTITATEFMNVLNSEKEAVINYEIHRINLAMARIEYLNICGKEIE"
     gene            complement(3520..4197)
                     /locus_tag="PLZ75_07495"
     CDS             complement(3520..4197)
                     /locus_tag="PLZ75_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOS72275.1"
                     /translation="MTEKDKLTEEKIFDSATEIFIEKGFDGARMRDIADHAGINKSLL
                     HYYYRTKERLFNAVFENIADRIFRKLIPVFDKNLSFEEKIRLFYREHIDLLHNNPRLP
                     VFLLNEIYRNPSRVEKLVKDTPIMKIWTVLSEQHSEELERFNVSADSIPQLMVSIASL
                     SLFPFIAKNIITAMFEKEDYDFDSFIEKRKEYAAEFVIRAIKNREQLPEADSKGQIRR
                     EKRTAGR"
     gene            4487..5683
                     /locus_tag="PLZ75_07500"
     CDS             4487..5683
                     /locus_tag="PLZ75_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72276.1"
                     /translation="MAKVAMLGAGFIGDFYTSSIHALRSRDRVHVIYSRDESRGKAFA
                     GKHGIPKWTTSMKEAVENPEVDAVVIGLPNNLHLEAVELAASAGKAVLCTKPLGRTAD
                     EALSMLEAVEKAGVFNGYLEDLAYPPKTLKALDAIKTGSLGQVIWVRSRESHPGPHSD
                     WFWTKELSGGGVLLDMGCHCIEIARNYIGKDIRPAEVMCVADTLVKPIDADDNSLGWV
                     RYENGAIGQFEASWTFRGGMDLRDEVMGTEGTVWINNFLRTGFEMFTAGDRKGYVAEK
                     AETEQGWLFPVGDELAELGYYNMFTDMFNAMENKLEPLETFYDGYIVNEIIDACYRSA
                     ETKKWEPVRLRVWRGREKTEPVSGIREYDRDHLFVKEERMPDGTVKLILREKKTGKIL
                     QKILKK"
     gene            5744..7141
                     /locus_tag="PLZ75_07505"
     CDS             5744..7141
                     /locus_tag="PLZ75_07505"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOS72277.1"
                     /translation="MKRIVYSSLALTLMLVLLQGCREKPWNGNDDPVIPRETLITNKW
                     IKDNMTDVYYWNDKMPKIDYTLEPDPEQYFRKLLYKDEDKWSRITSDYPSLKADLEGK
                     PLTMGYAPAFFLIGDNKIVIVVKYVYPGSAAAEVGLKRGDIILSIDNTQMDKSNYYDL
                     YSGDNYTVQMGRLEGNSLVPTGESKSLRARVTVTDPLIHHSVIDVDGHKIAYMVYVEF
                     IAGEGSKFLLEMDNILNSFKAEGVTDLVVDLRYNPGGEISAAVHLASEIAPWEVISGG
                     STLITLKYNKELEAYLRSDMQKYGSYLAHKFKTTSANLDLDRVFFLTTSGTASASELV
                     ITGLDPYMDVVKIGEATYGKYAGAWVIPDDNEKWAILPIVSKYSNNDGYTDFVNGLPP
                     DYEIEDDLFEAVQFGDTDDPMLAKAIELATGKKYAPKRMRKGEIPKYPEIMPERMKLR
                     SSLILQGCEINQYVD"
     gene            complement(7159..>8237)
                     /locus_tag="PLZ75_07510"
     CDS             complement(7159..>8237)
                     /locus_tag="PLZ75_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOS72278.1"
                     /translation="CPCALGLATPTAIMTGIGKGADHGILIRDAESLETAHRINALVL
                     DKTGTITLGEPAVKDLLWKQNTAEDVYGRILYSIQKNNDHPLAEPLVEFLENNGIMSN
                     SVPLSLNVITGRGISAVIDGSTYYVGNRSLMTEKGISVDEEIQEASSRWEDDAKTVSF
                     FSDWEQVLCAFAVSDRIRESSAEAIRQLHQMGIEVHMVTGDNRHAAAVMAAEAGIDSY
                     LAEALPQDKDDFILRLQEQGKVTAMAGDGINDSQALARSDVSIAMGKGSDIAMDVAKM
                     TIVSSDLSRIPQAIRLSKQTVRTIKQNLFWAFIYNVIGIPIAAGILYPFTGFLLNPMI
                     AGAAMALSSVSVVTNSLRIRRRPL"
BASE COUNT         2055 a         1902 c         1934 g         2346 t
ORIGIN      
        1 atatagccta tgttcctttt tatctgctcc gcttcgtttc ctgcatcaaa tccggaaacc
       61 ctgacaatcc cggatgtggg ttttgacagc cccgacaata tcctgagggc agttgtcttt
      121 cccgctccgt ttgcacccag gaaaccgaaa atctcacctt catacacttc aaaatccagt
      181 ccgtcgttgg ccgtgaagct tccgaatttt ttcacaagat tccttacgta tattaccgga
      241 atgtctttca tgtcttttat tatgtccctg acctgtcctt tcagcaggaa caggccggct
      301 gttttttcta tttgtcctgc atctctattt gtcctgcatc agttcaagga acctgtcttc
      361 gattcctgcc ctggtttcac ttattgttgc atcactgatt cccatgctgt tcaggtatcc
      421 ggtaagtgag tggtcctgtt cattgtccct gaaggtcaga tgtaccgaat ctccgaaggg
      481 gtaggctgta gcggttcccg ggaaatttct cagggcattt ataagtttgt acttttcaga
      541 agatttcaca ctgaaaattt tcctgctgaa ttcttctctg atcttttccg gaggatttac
      601 tgacaagatc tgtcctttct gaatgagtgc caccctgttg catcgcattg cctcgtccat
      661 gtagggagtg gatgcaatga ttgtgatatt ttcctccctg agcctgttga gcatttccca
      721 gaattcggag cgtgatacgg catcaactcc cgtagtcggc tcatccagta caagcagtcc
      781 gggtttatga ataagggcac aggagagggc aagtttttgt ttcatgcccc cggagagttt
      841 ccctgccagt cttttcctga aaggctcaat atgagaatag acatcccgga tgagatcata
      901 gtttttcctg acggtagttc cgaacaccgt ggcatagaaa ttcaggttct cttccaccgt
      961 aagatcatga taaaggctga accgtccggg catatagccg atattccttc ggagctccct
     1021 gtagccggtt ttgcagtcga ggcccatgac ggtcattctg cctgcgtcgg gaagcagcag
     1081 ggttgttatt attctgaaca gggtggtttt gccggcaccg tcagggccga taaagccgaa
     1141 tatctccccc ctgttcactt caaaggatat gtttttgaga gcttccgtat ctccgaagct
     1201 ctttcctatg ccctgtgcct caatgatttt ttccatgctt ttcagaagat tgcttccccc
     1261 ggcattccgg ctttcatgct gccgtcgttc cttacctcaa tgcttactgc atacacaagt
     1321 gttactcttt cttcctttgt ctggattatc ctgggcgtga attcagcctt ttccgatata
     1381 tggcttatcc tgccggggaa actcctgtat cctttttccc cctcatcaat cctgacggtg
     1441 cattcactgc cgactctgag agatcccagc tgtcctccgc tgacatacac cttcagcttc
     1501 agcaatgaca ggtcggctat tttaaccagt ggcctgccgg ctgtggtcat ttcccctgcc
     1561 tcggaatatt tttcaataag ggttcccccg atggggctcc tgacacagct gcgggccagc
     1621 tgttcagcga gtgttgcctt ttttgtttca aaaaccgtca gttcggcagc aacagatgcc
     1681 ttctgggtat tgtttgcagc agcctgtttc tgcaatactt ccacctggcc cgcaagatca
     1741 tcatactgct tctgtgtggc cgcatcattc ttcagcatgt tttcaatgcg ggcgatattc
     1801 accctgagat tggcaatctg ctgtccgata atgtcattct gggcatttat ggaagcgatg
     1861 cgggtactta cacttctcat ggcggcatct atttcagctt tctgaagatg gaaaagcatg
     1921 gtgtcaacaa gtgcaatctc atcacccttt tcgatttcag atccttctct ggcctcaaac
     1981 ctgaggatgc gcccgcttgt ctctgacgag acaattactt cagtggcttc aaaattgccg
     2041 tatgcatcag cttcaccgga tttgccgctg caggctgcaa gcattgatgc tgtaatgatt
     2101 accagggata tattcctcat aataaatgat tattgattta ttctgtcagt cttgggctta
     2161 tcggtttcac gccgtgcatc acgccgcgcc ggtttgttgt tattctatct cttttccgca
     2221 tatattcaga tattcaatcc ttgccattgc aaggtttatc ctgtgaatct catagtttat
     2281 aacagcctct ttttcggaat tgagtacatt cataaattcc gtggcagtta tcgtcccgtt
     2341 gtcatactgt gatcctgctg ctgcggtaat ccgtttcctc aggccgatga gttccgtatc
     2401 gctgaggagc agtgattcca gacttgaaat ttcagcactc ttggcatcca gaagcctttt
     2461 cagattgtct tccatgtcat tcttcctggc ctgtatcagg ttctgccgga gcgagactat
     2521 ctgtttttca tttcttgcct tgttccagtc gaatatgttc catctgattc ctgccccgag
     2581 aatataatat ggtgcaaatt catccctgaa gaaattgttt ccgggcggat ttccgtatcc
     2641 gaaagtggca aaaccgaatg ccctgggcat acgttcactg ttaatgaggc tcagggatgc
     2701 atcaagctgc tccctgcgga gatcgaaaat ccttaattcg ggacgtgcaa gttcatctgt
     2761 cagttcttcc tttaccggcg gcagcacaaa ccgaaccgag gtatctattt cagttccggt
     2821 gagagcggac aggattctga gaagggattt ttttctgatt ccgttttcgg tgagctgttg
     2881 ttcaagcttt aacctttccg atgaaagaac atcaatatcc gatttcagga tcataccgtt
     2941 caggactgcc gaattcagag aggcgattct tctatctatt acttccaggt agttttgcag
     3001 cagctctttc tgcctgtcga gcagcatcat actgaaataa tgactgttta cctgtgcacg
     3061 aagtttatag agatcggact cattctgttt tttgctgata tggagatccg cttcttccag
     3121 ggccctggca ctttttatgg caccaccgtc atatatcatc tgactgatat caagagtgat
     3181 cctgtactgt tcatggggga ggggtttcag aagcccgccg gttcccggaa ggggaagtga
     3241 acctattgca tcctccatgt tcacaactga tgaattatat atgaaactgc cgtttgcatc
     3301 aagcgacggg agccagcctt ttaaaagatt tctgttcctt atttgccaga tattgtcgta
     3361 aacctctttt tcggcagaca agggagctgt tgcatatgcc atttcatagc attccttcag
     3421 tgtaatgact ttctgggcct taagcggcat gatccctgtc aggacaataa aaagtaaaaa
     3481 atatagcttt tgcatgtcag tatattgttt catctgtgat tacctgcctg cggttctctt
     3541 ttcccggcgg atctgacctt tggagtctgc ttccggtaac tgctcccggt ttttaatcgc
     3601 ccttataaca aactctgctg catattcttt ccttttttcg ataaaactgt caaaatcata
     3661 atcctctttt tcgaacatgg ctgtgattat atttttagca atgaagggaa ataagctcag
     3721 tgaggcaatg gaaaccatca gctggggaat tgaatcagcg gacacgttga atctttcaag
     3781 ttcctcactg tgctgttctg aaagcactgt ccatattttc atgatagggg tatcctttac
     3841 gagcttctca acccttgaag gattccggta tatttcattc aggagaaaga cgggcagccg
     3901 gggattatta tgcagtaaat caatatgctc cctgtaaaaa agcctgatct tttcctcaaa
     3961 ggaaaggttt ttgtcaaaca cgggtatgag tttcctgaat atcctgtcag ctatattttc
     4021 aaaaacagca ttgaaaagcc gctcctttgt ccggtaataa taatgcagca gggacttgtt
     4081 tattcccgca tgatcggcaa tatcccgcat gcgggccccg tcaaagcctt tttcaatgaa
     4141 aatctccgtg gctgaatcga agatcttctc ttctgttaac ttgtcttttt cagtcatgga
     4201 atttaaccgt aaaatttaat caataagatt aaatatataa tttaaattta aaatttaatc
     4261 gtatggtcaa atttaaaaca aataattgac atgtatgact attcacctga aaaaatctat
     4321 ctttgattct gccgggaggg ttttatccgg cccagaagcg gagaggaaga aggtatgtga
     4381 tacggggaag tacaggaaca taccggcttt caggaccgga ttatttcaga ccggcagatc
     4441 tctgttttgt aggaaaacct gtaataatca ccaaaaaata acagatatgg caaaagtagc
     4501 aatgctcggg gccggtttta tcggcgactt ttacacttct tccattcatg ccctcagaag
     4561 ccgtgacagg gttcatgtta tctattcccg tgatgaatca cgcggaaaag cctttgcagg
     4621 gaaacacgga atacctaaat ggacaacctc gatgaaggag gcagttgaga atcctgaagt
     4681 tgatgctgtc gttatagggc ttcccaacaa tcttcacctt gaagctgttg agcttgccgc
     4741 atcggcaggc aaggccgttc tctgcacgaa gccccttggc cgcactgcgg atgaagcctt
     4801 aagcatgctg gaggcggttg aaaaagcggg tgtcttcaac ggttatctgg aagatcttgc
     4861 ttatcccccg aaaacactta aagcccttga tgcgataaaa accggcagtc ttggccaggt
     4921 tatctgggtg aggtcgaggg agtcgcatcc cggtcctcac agtgactggt tctggacaaa
     4981 agagctttcc ggcggcggtg tgctccttga catgggatgt cactgcattg agatagcacg
     5041 caattatatc ggaaaggaca ttcgtcctgc cgaagtgatg tgtgttgcgg acacccttgt
     5101 aaagccgata gatgccgacg ataattcact cggatgggtg aggtatgaga atggtgcaat
     5161 cggtcagttt gaagcaagct ggaccttccg cgggggaatg gatctgaggg atgaagtgat
     5221 gggaacggaa ggaacggtct ggatcaataa ttttctccgt acgggatttg aaatgttcac
     5281 ggcaggagac cgcaaaggat atgtcgccga gaaggcggaa actgagcagg gttggctttt
     5341 ccccgtgggg gatgaactgg ctgagctcgg ttattacaat atgtttaccg atatgttcaa
     5401 tgccatggaa aacaagctgg aaccgcttga aacattttat gacggatata ttgtgaatga
     5461 gatcattgat gcatgctaca gatcggcaga aacaaaaaaa tgggagcctg tcagactcag
     5521 ggtctggcgc ggcagggaaa aaacagaacc ggtttccggg atacgcgaat atgacaggga
     5581 ccatcttttt gttaaggagg aaagaatgcc ggacggaacg gtaaagctga ttctcaggga
     5641 aaagaaaacg ggtaaaattc tgcagaaaat tctgaagaaa tgacctgtgt ccttcattca
     5701 cagaatataa atatcgttac ttttgcggaa taatccgata tatatgaaaa ggatagttta
     5761 ttcttccctt gcactgactc tgatgctggt tctgcttcag gggtgcaggg aaaagccatg
     5821 gaacgggaat gatgatccgg ttattccgcg cgaaacgctg atcacaaaca agtggataaa
     5881 ggataacatg acagatgtgt attactggaa cgacaagatg ccgaagattg attacaccct
     5941 ggagcctgat ccagaacaat actttagaaa gctgctctac aaggatgaag ataaatggtc
     6001 gagaataacc agtgattatc cctcactcaa ggcagacctg gaaggcaagc cgctcacaat
     6061 gggatacgcc cccgcctttt ttcttatagg cgacaacaag atagtaatag ttgtgaaata
     6121 tgtgtatccg ggttcggcag cagccgaagt gggtctgaaa aggggtgata ttattctctc
     6181 catcgacaat acccagatgg acaaatcaaa ctattatgac ctgtattcag gtgataacta
     6241 cacggtacag atgggccggc ttgagggaaa cagccttgtg ccgacgggag agtctaagtc
     6301 cctccgggca agggtcactg ttacggatcc tctcattcat cactctgtga tagatgtgga
     6361 cgggcacaag atagcatata tggtatatgt tgaatttatt gccggtgaag gcagtaaatt
     6421 ccttctggaa atggataata tcctgaatag tttcaaagct gagggcgtta cggaccttgt
     6481 tgtggatctg cgttacaatc ccggcgggga aatcagtgca gcagtgcacc tggcttctga
     6541 aatagcaccc tgggaagtga taagcggagg aagtacactt attaccctga agtacaacaa
     6601 ggagctggag gcatacctga gaagcgacat gcagaaatat ggcagctatc ttgcccacaa
     6661 attcaagaca acatctgcca acctggacct tgaccgtgtg tttttcctga ccacttcagg
     6721 cactgcttcg gccagcgaac tggtgataac cggcctggat ccctacatgg atgtggtgaa
     6781 gataggagaa gcgacatacg ggaaatatgc cggggcctgg gtgataccgg atgataatga
     6841 aaaatgggca attctgccga ttgtttccaa atattcaaat aatgacggat atactgattt
     6901 tgtgaacgga ctgccgccgg attatgaaat tgaagacgac ctgtttgagg cagttcagtt
     6961 tggagacacc gatgatccca tgctcgccaa agccattgaa ctggcaacgg gaaagaaata
     7021 tgctcctaaa cgcatgagaa aaggggaaat ccccaaatat cccgaaataa tgcccgaacg
     7081 gatgaaactg agatcgagcc tgattttaca gggatgtgaa ataaaccagt acgttgattg
     7141 aaaagagaag gtctgtgatc ataaaggcct gcgcctgatc ctgaggctgt tggtcacaac
     7201 gcttactgaa ctgagcgcca ttgcggcacc tgcaatcatc ggattaagaa ggaatccggt
     7261 gaagggatat agtattccag cagctattgg tattccgatt acattataaa taaatgccca
     7321 gaacaggttc tgttttatcg ttctgacggt ctgcttcgac aatctgattg cctgaggaat
     7381 gcggctgaga tctgaagaaa cgattgtcat tttcgcaaca tccattgcaa tatccgagcc
     7441 tttccccatc gctatgctga cgtcagacct ggccagtgcc tgagaatcgt tgataccgtc
     7501 gcctgccatt gccgttacct tgccctgttc ctgaagccgg agaatgaaat catccttgtc
     7561 ctgcggaaga gcttcggcca aataactgtc aattcctgct tccgcagcca ttacagcggc
     7621 agcatgcctg ttgtcacccg ttaccatgtg aacttcgatc cccatctgat gaagctgtct
     7681 tattgcttca gcagaagatt cccttatcct gtcggatacg gcaaatgcac aaaggacctg
     7741 ttcccaatca gagaagaagg atacggtctt tgcatcatct tcccaccgtg aggatgcctc
     7801 ttgtatctct tcatcgacag atatgccttt ttctgtcatc agggatctgt ttcccacgta
     7861 ataggtactt ccatcaataa ccgcgctgat gcccctgccg gttatgacat tgaggctgag
     7921 cggaacggaa ttgctcatta ttccgttgtt ttcaaggaat tcaaccagtg gttccgcgag
     7981 aggatgatca ttgtttttct ggatggaata caggattctt ccatacacat cctcagctgt
     8041 gttttgtttc cacagcagat ccttcactgc cggctcgccg agtgttatcg ttccggtttt
     8101 gtcaagtaca agtgcattga tcctgtgggc tgtttccaga ctttcggcat ccctgatcag
     8161 gataccgtga tcagcaccct tgcctatacc tgtcatgatt gcagtggggg tggcaagtcc
     8221 gagggcacag ggacagg
//
LOCUS       DAOSPX010000127         8212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8254_length_8212_cov_4.976830, whole genome shotgun sequence.
ACCESSION   DAOSPX010000127 DAOSPX010000000
VERSION     DAOSPX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8254_length_8212_cov_4.976830"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLZ75_07515"
     CDS             <1..488
                     /locus_tag="PLZ75_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOS72279.1"
                     /translation="ELRRGIFVPDASFGIYLLNHRYNAGFSAEQLFGGSTKIGSRAYK
                     YFKMYRHYYLFGSYSFELNSMMGLEPAFLIMMSEQLRPQADIGLTYSYNNGFWTGLSW
                     RTNGAIIANVGVKHNRMFIGYAFDFTLQEIQRITYGTHEITLAVKLGDSARKYRWLDR
                     Y"
     gene            complement(572..1474)
                     /locus_tag="PLZ75_07520"
     CDS             complement(572..1474)
                     /locus_tag="PLZ75_07520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS72280.1"
                     /translation="MVHLSKCPLCDSGNITNYLKCRDHFLSGEIFELSRCRSCGFVFT
                     QDHPDEKDSAKYYESDEYLSHNTGSGGFVSLIYRISRKIMLTRKRRLIVRAAGMKTGR
                     LLDMGSGSGHFLSAMKNAGWDVTGMEINDKARSLSEELFSLKVIPPGRIDTLETGTYD
                     CITLWHVLEHFHDPFFYAREIKRLLKDEGICIVALPNAGSYDAAHYSGSWAAYDVPRH
                     LWHFNPETFRLFSGKAGFHVKKILPLPPDVFYISALSEKYKGSGIFFIKGIIRGIWFS
                     VRSLFRKHRSSSLVYILGNKTDSA"
     gene            complement(1458..3938)
                     /locus_tag="PLZ75_07525"
     CDS             complement(1458..3938)
                     /locus_tag="PLZ75_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72281.1"
                     /translation="MPINVKIRNFLPHVLAVIFFIVLSYMYFYPVLEGKILMANDSNV
                     SRYASKEIVDYREQSGREPLWTNAMFSGMPAYLISVKHPGNLVKPLDLALRVFKMPVS
                     VLFLAMTGFYILLLMFGMNRWIAVTGAVAWGFSSFLFLILAAGHNTQAIALVYMAPMI
                     GGVWYAYRRNAIKGALFTAFVLSLELVANHPQITYYAVICLLVFIITEFIRSLREKEI
                     PAFLKTSALLVVPVIIALGINFGNLYTVYEYSKYSMRGKSDLVIETGNQSKGLDRDYI
                     THWSYGIDESMNLLIPNYKGGSSRPFDRDSRTVRALRQNDLASASGQVLKYWGTQPGT
                     DGPHYMGAIVIFLFILGLIITRGPEKWWLLIATVLSLMLAWGKNFMPFTNLFIDFFPG
                     YNKFRAVTMTLVIAQFCIPLLAALALRDVFESRVTGKDLMKGLKIASGISAGILLLVI
                     IFPGIAGSFLNEGEAPYPEWLRTAMIADRKELLRTDAIRSLAFILAAAAIVLAFVKDK
                     LRKEHSVILIGMLILLDLWNIDKRYLDAGRFEKPSSFQKSVTPSAADAFILNDGSYYR
                     VLNLAVSTFNDNTPTSYFHKSIGGYHGAKLKRYQELIDSAIIRDLIIFTESARDATSA
                     DDLVTALAGTPSLNMLNTKYIIYNPEAPPVINPYHSGNAWFVENPVMAANANEEIGAI
                     KNLDPSDRAIIDKQFADLVPAAAYPLSDGDTISLVSYKPDELVYSYSAADEKLVVFSE
                     IYYPAGWKCYVDGTETPYFRANYVLRAMVAPPGEHEIRFSFEPASYITGNRVSLAASV
                     LLILLTAGYFASGFVMKPKSGSVNGSSQ"
     gene            complement(4020..5537)
                     /locus_tag="PLZ75_07530"
     CDS             complement(4020..5537)
                     /locus_tag="PLZ75_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOS72282.1"
                     /translation="MLKSIKLTVKDSLIYGFGNIAVMLVGLVLIPLYTDPKYFTIEEF
                     GIIGILDITRTVLTTFLTFSLPNSFFRWYWDKDHRDNQKGLFFMTLMSQIVITGSLCI
                     LLIPLSGKLSGILFKTDEWTNVIILLIISSASQAINYLISVLLRLESRSGLFTLTNLF
                     KLIVVLALTIYFIIVKKMGIEGIYLAQVIGNMLYILVLSGFIVKNCKISFNGKVLREM
                     HVYGFPLFLGSIASILMNVVDRYALNTLSVLRSVALYTLAVKLANVIKLIVVDSVRLT
                     IMPVFMKKMDSPDNRRFQSKILLYTSLVVMYAIVALSLFSLEITKVITSSRAYWNIVY
                     IMPVLSLSVFFVSMKEITVYGLYIAKKSRTISYIVISATILNIVLNIILIPLWDVTGT
                     ALATLLSQAYFWFMCYFFSQKEYHVPYETGKLIILFVTGTLFAFSGLLLVNVDTIPRL
                     IIKLFLVAIFPFILYILNFYEKAEINAVRGFVAKWSDLRRLGDNLKSLKNITDDL"
     gene            5711..6214
                     /locus_tag="PLZ75_07535"
     CDS             5711..6214
                     /locus_tag="PLZ75_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72283.1"
                     /translation="MADPLKKRYAITLSFVRKHFNREDLILDLGVNNDLAGYLRADGF
                     KIQNTSGEDLDVDYKFISDYKYITAFEIFEHMFAPFNLLREASGKLIASVPLRLWFAP
                     AFWDYNDKLNCHYHEFEVRQFNHLMDRTGWKIIDSVTWRSWDNTIGIRPFLRRIYPRY
                     YMVYAEK"
     gene            complement(6339..7520)
                     /locus_tag="PLZ75_07540"
     CDS             complement(6339..7520)
                     /locus_tag="PLZ75_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS72284.1"
                     /translation="MNVLMLLENDYEKDLRVKREVRALHEAGFRVTVAAISRERDFPP
                     EQRDNCLLYKRKIPVFIYKSSVAALKLPFYFNFWRKYVREIVRDYPADIIHVNDLPLA
                     RIGLEMKKAGNVKFVLDLHENWPGLLRNAQHTRTLAGRLASSDKQWVRYEKDMVQEAD
                     MVITVVEEAGDRVAALTDRPEKIYIVSNTIDTENISFYERQGKNREFTLFYGGAINKH
                     RGLQIVIQAIKILKDRNVPVSLQIIGTGSYRKVLEKEIEKNDLSSRIEFHGYKPLTAM
                     LEMLSHADAAIIPHLRNENNDASMPNKLFQYMFSATPVISSDCISLKRIILETGAGFI
                     YRNDSPSELASLLEQLSHNRDMTGERGKKGREAVLARYNWNHDRKQLIDAYKNLSKTD
                     T"
     gene            complement(7577..>8212)
                     /locus_tag="PLZ75_07545"
     CDS             complement(7577..>8212)
                     /locus_tag="PLZ75_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72285.1"
                     /translation="KLADGQLPEKVITVTNGYDPGDFKHMNAVPESFAITYLGTFAAD
                     YDAETFSDAADYFTAHSSGVINLRFIGKTDSRTERLFSGRKNLNLQVMPYAEHDKALE
                     YLAGSAALLLVVPSGSKETEMIPGKLFEYLASKRRIIALGPEDSDVADILRKTGGGRI
                     FSRGDSAELGKYLISLYDDFKNGNFEAASAGLEEYSRPALAEKIAAVLNSL"
BASE COUNT         2165 a         1866 c         1964 g         2217 t
ORIGIN      
        1 atgaactgcg ccggggaata ttcgtaccgg atgcatcatt cgggatatat ctcctgaatc
       61 acaggtataa tgcgggattt tcagctgaac agctcttcgg gggctcgacg aaaataggca
      121 gccgggcata taaatatttc aaaatgtaca ggcattacta cctgttcgga tcatactcct
      181 tcgagctgaa ttcaatgatg ggcctggagc ccgcgttcct catcatgatg tcggagcagt
      241 tgaggccaca agcagatatt ggccttacct attcctataa caacgggttc tggaccggcc
      301 tttcctggag gacaaacggg gcaatcatag caaatgtggg cgtaaagcat aacaggatgt
      361 tcatcggata tgcatttgat ttcacgctcc aggaaatcca gagaataacc tacgggacac
      421 atgaaataac gctggcagtc aagctgggag acagtgcaag aaaataccgc tggctcgacc
      481 gctattaaaa agcacagcat ccctgtctgt ttttccggcc tgccgggctg ttaaccggca
      541 gaatgaattg ttgcaggggg tcgttttagc gttaagcaga gtcagttttg ttcccaagta
      601 tgtaaaccag tgaactggat ctgtgttttc tgaaaaggga gcggactgaa aaccatattc
      661 cccggataat tcctttaatg aaaaagatgc cggatccttt gtacttctcg ctgagagcgg
      721 aaatataaaa tacatccggg ggaagcggaa gaatcttttt cacatggaat cctgccttcc
      781 cggaaaacag tctgaatgtt tccgggttga aatgccataa atgccttggc acatcatatg
      841 cagcccatga tccggaataa tgggcggcgt cgtatgatcc ggcattcggc agtgccacaa
      901 tgcagatacc ttcatccttg agcagccgct ttatttcccg tgcataaaaa aagggatcat
      961 gaaaatgttc cagcacatgc cacagggtga tacagtcata ggttcccgtt tccagagtgt
     1021 caatcctgcc ggggggaatc actttcaggc tgaacagctc ttctgacagg ctgcgtgcct
     1081 tgtcatttat ttccattccc gtaacatccc agccggcatt tttcatcgct gacagaaaat
     1141 gtccgctgcc gcttcccatg tccagaagcc tgccggtctt catgccggca gccctcacta
     1201 tgagtcttcg tttgcgggtc agcatgatct tccttgagat ccggtaaatc agactgacga
     1261 agcccccgga gccggtattg tgcgacagat attcatccga ttcataatac ttcgccgaat
     1321 ctttttcatc gggatgatcc tgtgtgaaga cgaaaccgca tgatctgcat cgggaaagct
     1381 caaatatctc cccgctgaga aaatgatccc tgcatttcag gtagttagtg atgtttcccg
     1441 aatcgcagag cggacattta ctgagatgaa ccatttactg acccggattt cggtttcatt
     1501 acaaagcctg aggcaaaata tccggccgta agcaggataa ggagaactga agcagccagg
     1561 gaaaccctgt ttccggttat gtatgatgca ggttcaaatg aaaatcttat ttcatgttct
     1621 cccggaggtg caaccattgc cctcagaacg tagtttgccc tgaaatacgg cgtttcggta
     1681 ccgtcaacat aacatttcca gcctgccgga taatagattt ccgaaaaaac caccagtttc
     1741 tcatctgcgg cggaatagct gtaaacgagt tcatccggct tataggaaac aaggctgatg
     1801 gtgtcgccgt ccgaaagggg ataagcggct gcaggtacaa gatctgcgaa ctgtttgtcg
     1861 attattgccc ggtcagaggg gtcaaggttt ttaatggccc ctatttcctc gtttgcattg
     1921 gcagccatta cggggttttc cacgaaccag gcatttccgg agtgataggg gttaatgacg
     1981 gggggagcct cgggattgta tattatgtat ttcgtattga gcatgttcaa tgaaggagtt
     2041 ccggccagtg cagtgacaag gtcatcagct gatgtggcat cccgggccga ttccgtaaaa
     2101 atgatcaggt cccttataat tgccgaatca atgagttcct ggtatctttt cagcttggcc
     2161 ccgtgatagc cgcctatgga tttatgaaaa taggatgtgg gggtattgtc attgaatgtt
     2221 gatactgcaa ggttcagtac cctgtagtac gatccgtcat taaggatgaa ggcatcggca
     2281 gcagagggag tgaccgattt ctgaaaagat gagggttttt caaaccggcc ggcatcaaga
     2341 tatcttttat ctatgttcca taagtccagc aggataagca tacctatcag aatcaccgag
     2401 tgctccttcc tgagcttgtc ctttacaaat gccagtacaa tggcggcagc ggcaagtatg
     2461 aaagccagcg aacggatggc atcggtcctc agaagctctt tcctgtcggc aatcattgcg
     2521 gtcctgagcc actcgggata gggtgcctca ccctcgttga ggaatgatcc tgctattccg
     2581 ggaaatatta tcaccagcag aaggattccg gctgatatcc ccgaagcaat tttaagtcct
     2641 ttcatcagat cctttcctgt taccctggat tcaaaaacat cccgcagggc cagggctgcc
     2701 agaagcggta tgcagaactg ggctatgaca agggtcatgg tcactgcccg gaacttgttg
     2761 tatcccggga aaaagtctat gaacaggttg gtgaatggca tgaagttttt tccccatgca
     2821 agcatgagcg agaggacggt ggcaatgagc agccaccatt tttccggtcc cctggtgata
     2881 atcagtccca gaatgaaaag gaagatcact atggcgccca tatagtgagg tccgtctgta
     2941 ccgggctggg taccccagta tttcagtacc tggccggaag ccgaagcgag atcattctgc
     3001 ctgagggccc tcacagtcct tgagtcacgg tcgaacggcc tgctggaccc tcccttgtag
     3061 tttgggatca gcaggttcat gctttcatca atgccatagc tccagtgggt tatataatcc
     3121 ctgtcgagtc cctttgactg gttcccggtt tcgatgacga ggtcggactt gccccgcatt
     3181 gaatatttgc tgtattcata aacggtgtaa aggttcccga agtttatgcc cagggcaatg
     3241 ataacgggga caacgaggag ggccgaggtt ttcaggaagg cgggaatctc cttctccctg
     3301 agcgagcgga tgaattcagt gattatgaac accagcaggc atattacggc ataatatgtt
     3361 atctgcggat ggtttgcaac cagttccagt gagaggacaa aggcagtgaa aagagctcct
     3421 tttatggcat tgcgcctgta tgcataccag actccgccaa tcatcggagc catgtaaacc
     3481 agggctattg cctgggtatt atgccccgct gccagaataa ggaacaggaa ggatgagaag
     3541 ccccaggcta cggctcctgt cactgctatc cagcggttca tcccgaacat gagaagcagg
     3601 atgtagaatc ctgtcatcgc gagaaagaga acggaaaccg gcattttaaa aaccctcagt
     3661 gccagatcga gaggctttac aagattaccc ggatgtttta ccgaaatgag ataggcaggc
     3721 attccgctga acatggcgtt tgtccataaa ggctccctgc cggactgctc ccggtaatca
     3781 acaatctctt tggaggcata tctggatacg ttcgagtcat tggccatcag tatcttgcct
     3841 tcaagtaccg gataaaaata catatatgaa aggactatga aaaagatcac agccaggacg
     3901 tgaggcagga aattcctgat tttaacattg atcggcatat acggagtttt gtcggaagta
     3961 aaaattaata atcagttcaa tataatacaa aaaggaggca atttacatat tccgcgacat
     4021 tatagatcat cggtgatgtt ttttaatgat ttcaggttgt cgcccagccg ccgcagatcg
     4081 gaccatttgg caacaaagcc cctgacggca tttatttctg ccttctcata aaaattcaga
     4141 atgtaaagga tgaagggaaa tattgcaaca aggaagagtt ttatgatcag ccggggtatt
     4201 gtatcaacat ttacgaggag aagtccggaa aaggcgaaca gtgtcccggt tacaaaaagt
     4261 atgatcagct tccccgtttc atagggtacg tggtattcct tctgggagaa aaaatagcac
     4321 ataaaccaga aatatgcctg ggacaaaagc gttgcaaggg ctgttcccgt tacatcccac
     4381 agggggatga gaattatgtt cagaactata ttgaggattg tagcagaaat tacaatatag
     4441 ctgatagtcc tgcttttttt ggcgatatag aggccatata ccgtaatttc cttcatggaa
     4501 acgaaaaata ccgaaagtga caggacgggc attatataaa caatattcca gtaagccctg
     4561 gaactggtga ttacctttgt tatttcaagt gagaaaaggg agagggctac tattgcatac
     4621 attaccacca gggaagtgta caggagtatc tttgactgga atctcctgtt gtccggggaa
     4681 tccattttct tcataaagac gggcatgatg gtgagcctca cggaatcaac gacaatcagt
     4741 tttatgacgt tcgccagttt caccgccagg gtatacagtg ccactgatct gagaaccgac
     4801 aaggtattga gtgcatatct gtcaaccaca ttcataagta tggatgcaat gcttcccagg
     4861 aagaggggaa agccgtagac atgcatttcc cggagcacct ttccgttaaa gctgatcttg
     4921 cagtttttta ctatgaatcc cgagaggacc aggatataaa gcatatttcc gataacctgt
     4981 gccaggtaaa taccttcaat gcccattttt ttcactatta tgaaataaat ggtaagcgcc
     5041 agcaccacta tcagcttgaa caggttggta agggtgaaca ggccggatcg tgactcaagc
     5101 ctgaggagta cacttataag gtaatttatt gcctgcgatg cagatgaaat aatcagcagg
     5161 ataattacat ttgtccattc gtctgttttg aacaggattc cggataattt tcccgaaaga
     5221 ggaatcagca ggatgcagag ggagccggta atgacaatct gcgacatcag ggtcatgaaa
     5281 aacaggcctt tctgattatc cctgtgatcc ttgtcccagt accatctgaa gaagctgttg
     5341 ggcagcgaaa aggtcaggaa tgttgtcagt acggttctgg tgatatcgag tatcccgatt
     5401 attccgaatt cctctatagt gaaatatttc gggtcggtgt acagggggat cagtaccagg
     5461 ccgacaagca tgacggcaat attgccaaat ccatatatga gtgaatcttt taccgtcagt
     5521 tttatagact tgagcatata gaattacaga tcattgggct aacccgccgt acaaatattt
     5581 tgaaaacctt aaaattacat ttttaatccg tatgtgaatt ccactgtacc gataattaaa
     5641 ctaaaagcta tttaatttaa atttgccgca acggttttaa caggaataac agcagactca
     5701 tttttcagct atggccgatc ctttaaagaa aagatacgcg ataaccctct cgtttgtaag
     5761 aaagcatttt aacagggaag atctgatact ggatctcggt gtaaacaatg atcttgccgg
     5821 atatctccgg gcggacggat ttaagataca gaacacaagc ggagaggatc tggatgttga
     5881 ctataaattc atcagtgatt ataagtatat cactgcattt gagatttttg aacacatgtt
     5941 cgcacccttt aacctgctcc gggaagcctc gggaaagtta attgcctcag tgcccctgag
     6001 attatggttt gcccctgcat tctgggatta caacgataag ctgaactgcc attatcatga
     6061 atttgaagtc aggcagttta atcatctgat ggacagaacc ggctggaaga taattgattc
     6121 agtaacctgg cggtcctggg acaatacaat aggaataagg cctttcctga gaaggatata
     6181 tccgcgctac tacatggttt atgctgaaaa ataagcaaat ctgttgagga tgcttttttt
     6241 ggcaagcgct attctttcaa ggagggggca ggaaaaccgc tgataagcga ttctgcatgg
     6301 tttttatcgg aaaataaccg ggattattac gggaaccttc atgtatcagt cttcgacaga
     6361 tttttataag catcaatcag ctgtttcctg tcgtggttcc agttatagcg tgccagcaca
     6421 gcctccctgc cttttttccc cctttcaccg gtcatatccc tgttgtggct gagctgctca
     6481 agcagtgatg caagttctga aggggaatca ttcctgtata tgaatccggc accggtttca
     6541 aggattatcc ttttaagcga gatacaatcg gatgagatga ccggcgttgc actgaacata
     6601 tactggaaaa gcttgttggg catgctggcg tcattgtttt cattcctcag gtgcgggatt
     6661 atggctgcat cggcatgtga aagcatctcc agcatcgcgg taagaggttt gtatccgtga
     6721 aattcaatcc gggatgaaag atcatttttt tcaatctctt tttcaaggac ctttctatag
     6781 ctgccggttc cgatgatctg aaggctgacc ggaacatttc tgtctttcag gatttttatt
     6841 gcctgtatta ctatctgaag gccccggtgt ttattgattg ccccgccgta gaacagggtg
     6901 aattccctgt ttttcccctg cctttcataa aaggaaatat tttccgtatc aatggtattg
     6961 gaaactatgt atatcttttc cggtctgtct gtcagtgctg ccacgcggtc cccggcttcc
     7021 tcaacaacgg taatcaccat atcggcttcc tgaaccatgt ctttttcata ccttacccat
     7081 tgtttgtctg acgaggccag gcgtccggcc agtgtccggg tatgctgtgc atttctcagc
     7141 aatcccggcc agttctcatg aagatcaagt acaaatttaa cattccccgc ttttttcatt
     7201 tcaagcccga tccgggccag gggcaggtca ttcacgtgta tgatatcagc cggataatcc
     7261 ctgacaattt ccctgacgta ttttctccag aaattaaaat agaagggcaa tttcaatgcg
     7321 gccacgctgg atttgtatat aaagaccgga attttccttt tgtaaagcag gcagttgtcc
     7381 cgttgttccg gcgggaaatc cctttcccgg gaaattgcag ccactgtcac cctgaatcct
     7441 gcttcatgaa gtgcccggac ctctcttttt accctcaggt ccttttcata atcgttttcc
     7501 agaagcatta aaacattcat gcgggcatat tttttcagtt gcggcagtgc ttttgttccc
     7561 ggcaggcggc actgactcag agagaattca gcacagcggc aattttttcc gccagggccg
     7621 gcctggaata ctcttccagt cctgcagatg ctgcttcgaa attaccattt ttaaaatcat
     7681 cataaaggct gatgagatat tttcccagtt ccgcggaatc accccgggag aatatcctcc
     7741 ctccgcctgt tttccggaga atgtcagcaa catcactgtc ttccggtccc agggcgatta
     7801 ttcttctttt tgatgcaaga tattcaaaca gttttcccgg tatcatctcg gtttccttac
     7861 tgccggaggg aacaaccaga aggagtgctg cggatccggc aagatattcc agtgccttgt
     7921 catgttccgc atagggcatt acctgaaggt tcaggttttt cctgccgctg aacagccttt
     7981 ctgtcctgct gtcggttttc cctatgaacc gcaggtttat aacgccggaa ctatgggctg
     8041 taaaataatc agctgcatct gaaaaggttt ccgcatcata atctgccgca aatgttccga
     8101 ggtaggtgat ggcaaaggac tccggcacgg cattcatatg tttgaaatca ccgggatcgt
     8161 atccgttggt gactgtgatc actttttccg gcagctggcc atctgccagt tt
//
LOCUS       DAOSPX010000128         8207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8257_length_8207_cov_5.271099, whole genome shotgun sequence.
ACCESSION   DAOSPX010000128 DAOSPX010000000
VERSION     DAOSPX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8257_length_8207_cov_5.271099"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..872
                     /locus_tag="PLZ75_07550"
     CDS             291..872
                     /locus_tag="PLZ75_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HOS72286.1"
                     /translation="MISETLNFLATELNKFLNQKMGPTTDPRLLVGNIIKAFEGESGK
                     DPVNKIFLSLINIEEDKVAKISENYTKTETAVIYKNPPLFVNLYILIAANLGNYSDSL
                     KAINFIMQFFQSQNCFTPVTHPGLDSRISQISADLFSLNFEQINHIWSTLGGKYLPSV
                     MYKLRQLKIEDEEAAAAEGRLIETIITETRAGR"
     gene            872..2068
                     /locus_tag="PLZ75_07555"
     CDS             872..2068
                     /locus_tag="PLZ75_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72287.1"
                     /translation="MNVRYGILFSVAILNDYFSSGKWKEISFLPLPETEKLIGSGGFR
                     IIKVHNQLLVVTRVDKDMKPLRLPDFFTGFSFFLQPQTPNYINFSNLPFRESRIKSLH
                     FHNLHNNVSGGVNYLSSAIENYSPVITYKPGEFCLGSDRSVYESIKINNNIQAPDDTD
                     TGSKEYWVKHGDTQLVSERDTIAVDADESFEIKKTGPVFNFKAAVKRTVHNVNVYACN
                     FSSGTYSNEVYHDIVRFDSPHDMVQVDMRSLSSGLYRIRVENEVNFVYMVSSGIDSDS
                     PFFLEIFNLPPSDPRSLVMPDGTLKNIKYTIHFASRRVFWRYRTRTTVIDNIKDSAGE
                     FKFKPDGPRFFISETPIPFSDTPRKTFSADSGNLVIASPLPNPMADRLLNKRDEIFTT
                     EISVNS"
     gene            2089..4023
                     /locus_tag="PLZ75_07560"
     CDS             2089..4023
                     /locus_tag="PLZ75_07560"
                     /inference="COORDINATES: protein motif:HMM:NF037565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HOS72288.1"
                     /translation="MATVYKTPGVFIEEISKFPPSVAQVETAIPAFIGYTEKAEDLAP
                     DDLIKKPRRIGSLVEFSYYYGNGPALKVSEVAIDESNNFKSAKITSDYYMYDSLRLFF
                     ANGGGDCFIVSIGKYPAAVTAGDFTAGIDTLLKEDDPTILLFPDAVTLGTSDLAAVQT
                     HALNHCGDESRLDRVTVFDLKEDDPKGATFRSNIGTSFLSYGIAYTPWLKTNLPKKVT
                     FPDIKDVIKKNGASVTLKGLTGDPAIQTQIDELEKAYADKTKIISGTDTLASPSANLR
                     DKYNSVVATYNAGKTTGNLQAVLTYVLSIIWKTDDYLKTGNAAELTNTALKNSVTANI
                     STFSTEAKKIIGLDKDLKAKFGAGYTTQAKAADFTDGIWGAGFVAGTAASGLLTAATD
                     EENLELALNALNNIFDTLSRAYLSLIVNAAASNARDADTALSLAFPLYKNILKGVSTY
                     SASIPPSGAIAGIYAMVDRTRGVWKAPANVSLANVIEPTVTFTKSELDALNVDVNAGK
                     SINAIRTFFGKGTLVYGARTLAGNDNEWRYVPVRRLFIMVEESVKKATEQFVFEPNDA
                     NTWVKVQAMIENFLHIIWRQGALQGAKPEHAFYVAVGLGKTMNPIDILEGRMIIEIGM
                     AAVRPAEFIIIRFSHKMAES"
     gene            4074..4502
                     /locus_tag="PLZ75_07565"
     CDS             4074..4502
                     /locus_tag="PLZ75_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOS72289.1"
                     /translation="MANYPLPKFHFMVEWGGNKIGFTEVTGLDMQVEAIEYREGSSKT
                     YSKVKMPGLHKYSNITLKRGTFEGDTDFYTWINTINLNTVERRDIVIKLLNEKHEPVV
                     TWRAVNAFPLKVQASDLKADGNEVAIESIEIAHEGLSIVK"
     gene            4544..5005
                     /locus_tag="PLZ75_07570"
     CDS             4544..5005
                     /locus_tag="PLZ75_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOS72290.1"
                     /translation="MADSKLPSAGGYYPPLGFHFKVEFQGIGNDNDTRFQSVSGLNIE
                     YETESIKEGGQNHFEHKLPVRSKFSDLVLKRGMLTDSEVIGWCKKALFDREITPVQVI
                     VILLNPEHQPLRTWKVNNAWPRKWNVSDFNAGENSIVVETLELCYSYFTVE"
     gene            5017..5190
                     /locus_tag="PLZ75_07575"
     CDS             5017..5190
                     /locus_tag="PLZ75_07575"
                     /inference="COORDINATES: protein motif:HMM:NF039278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HOS72291.1"
                     /translation="MPVQITELNISVSVNQPGDQKSPGAQTQNPDDQGSAENIVDECV
                     EQIMKILNDKKER"
     gene            5190..5876
                     /locus_tag="PLZ75_07580"
     CDS             5190..5876
                     /locus_tag="PLZ75_07580"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72292.1"
                     /translation="MEDRGEIKKMWIRSYNSAKMEDKDFAGEFYAMVNPENYTIDLKM
                     ETKGGQGQGTSGKQQKFSFKQPEEFTFEFLFDNTGIIDGKPKKAGIADDLKKLQDLAF
                     KLDGKVHEPRHIHLIWGTLDFKGRITGMTINYKLFNPDGSPIRATVKVTIKKSIEEKE
                     RTAEERKSSPDLTHRRIIRKGESLPWLCNIIYGDPKYYIQVARANHISDFRNIKEGTE
                     IFFPAFAKNE"
     gene            5869..7617
                     /gene="vgrG"
                     /locus_tag="PLZ75_07585"
     CDS             5869..7617
                     /gene="vgrG"
                     /locus_tag="PLZ75_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HOS72293.1"
                     /translation="MSNERIIPSDQPKSVCTFTILSDGSAVPSAWHVLSIVVYKEVNR
                     IPSATIILIDGDVATETFEISNSDDFKPGRKIEIKAGWVSNEETIFKGMVVKQNIKVR
                     KSNSVLMVECRDEAMKMTIAPKSKYFNDKKDSEIFEEIINDYGLTADVEATTLQHREL
                     VQYNSTDWDFMLCRADVNGKLVIADDGKITIKKPDVSATPELTVQYGSTVLDLDAEID
                     ARLQLPGVKAAAWNYTDQELIDTVEGSEPPVPAAGNLSPDDLKIASSPGSPVMIHSGR
                     ITEAELQQWADARLQKNRLAKIRGRVKVEGYAKIKPGKILQLNGVGTRFEGRLIVSGI
                     RHQIENGNWTTNIQFGTSSERFAETFNVQQPLAGGMLPAIRGLQTGIVTRLEDDPDGE
                     DRIKVRLPVIHKSEEGIWCRVASPDAGKERGLFFRPEIGDEVIVGFIDEDPRHAVVLG
                     MLNSSKLPAHTQAKDTNHLKGYVSREKIKLMFDDEKKVITMETPGGNKIVISDEDKGI
                     TLTDQNGNKIVMNNDGISVESAKNMSLKASNDLKAEGLNAEIKASAQIKVKGEAGAEY
                     ASGGNTSVKGSVVNIN"
     gene            7639..7932
                     /locus_tag="PLZ75_07590"
     CDS             7639..7932
                     /locus_tag="PLZ75_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HOS72294.1"
                     /translation="MASAARVGDMHTCPMVLPGPVPHVGGPVLPPGTPTVMIAGMPAA
                     TVGDMCTCTGPPDSIVKGSATVMIGGKPAARMGDSTAHGGVIIAGAPTVNIGG"
     gene            7937..>8207
                     /locus_tag="PLZ75_07595"
     CDS             7937..>8207
                     /locus_tag="PLZ75_07595"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOS72295.1"
                     /translation="MDESSDDRNFLGRGWSFPPQFDPVSKTVIMTEEAEDINNSLSIL
                     LSTIVGERVMDMSYGCNLEDMVFETLDTSVITYLKSKITKAILFYE"
BASE COUNT         2499 a         1668 c         1961 g         2079 t
ORIGIN      
        1 gtccgcccgg agaattttta tttgacttgg ggtttcgtat ttcgtaactt taacaataga
       61 ataaaaaacc ttcttccaaa gtaccagatt tcagaaattc cagggataag cgttgaatta
      121 aacctaatac agttcgccat gaaattgaaa atctgctact tcggttgcct gaacagtctg
      181 ttatagcaga acctgttttt ttaaaaaaca actgaacttt ttttacccgg taaatgttat
      241 taataaggcg agttttctga attggatctg gtttgttaaa tacttcccgt atgattagtg
      301 aaacgctgaa cttccttgcc accgaactca acaagttcct gaatcagaaa atgggtccca
      361 caaccgatcc caggctcctg gttggtaata taataaaagc atttgaagga gagtcaggta
      421 aagatcctgt taataagatc ttcctttcat tgatcaatat tgaggaagac aaagttgcaa
      481 agatcagcga aaattacaca aagacagaaa cggcggttat ttacaagaac ccgccattgt
      541 ttgtcaatct ttatatcctt atagctgcca atctgggaaa ttacagtgac agtctgaaag
      601 ctataaattt cattatgcag ttttttcagt cacaaaactg ttttacgccg gttacacacc
      661 ccgggctcga tagcaggata tcacagataa gcgccgatct gttctcactt aattttgaac
      721 agatcaacca tatctggagc acactgggtg gcaagtacct tccttccgta atgtataagc
      781 tgagacaact gaagatagag gatgaagaag ctgctgccgc agaaggcaga ctgattgaaa
      841 cgataataac tgaaacaagg gcaggacgct gatgaatgta agatacggaa tattattttc
      901 ggtcgcaatt cttaacgatt atttcagttc gggtaaatgg aaggagatct cttttttacc
      961 gctgccggaa acggaaaaac ttatcggttc cggaggcttc aggataatta aggttcataa
     1021 tcaattgctt gtggttacaa gagtggataa agatatgaaa ccactcaggt tacctgattt
     1081 ttttaccgga ttttcatttt ttcttcagcc gcagactccg aattacataa acttctcaaa
     1141 tctcccgttc cgggaatccc gtataaagag cctgcatttt cacaatctgc ataataatgt
     1201 ctcaggcggt gtgaactacc tttcatccgc aatagagaac tactccccgg ttattactta
     1261 taaaccggga gaattttgcc tgggttccga caggagtgta tatgaatcaa ttaaaataaa
     1321 taataacatc caggctcctg atgataccga taccggttca aaggaatact gggttaaaca
     1381 tggtgatact cagcttgtaa gtgaaagaga tactattgct gtcgatgcag atgaaagttt
     1441 tgagatcaaa aaaaccggac cggtatttaa ttttaaggct gcagtcaaaa ggacagttca
     1501 taatgtgaat gtatatgctt gtaatttttc ctcaggtact tacagcaatg aggtttacca
     1561 cgatattgtc cgcttcgaca gcccccatga catggtgcag gtagatatgc gctccctgtc
     1621 ctcaggatta tacaggatca gggtggaaaa tgaagtcaat tttgtttata tggtttcctc
     1681 gggcattgat tcggattcac ctttttttct tgagatattt aatctgcctc catctgatcc
     1741 gcggtcactt gtcatgcccg acggtacatt gaagaacata aaatatacca ttcattttgc
     1801 cagcaggagg gtcttctgga ggtacaggac cagaacgaca gttatagata atattaagga
     1861 ctctgcaggt gagtttaaat tcaaacctga cggaccgaga ttttttattt cggagacgcc
     1921 catccctttc agcgatactc caaggaagac cttcagcgct gattcaggta acctggttat
     1981 agcctcgcca ttgcctaatc ctatggccga caggctttta aataagcgtg atgaaatttt
     2041 caccacagaa atatccgtta attcctaaaa aatttaaatc cgaaatttat ggcaactgtt
     2101 tacaaaacac ctggtgtttt tatagaagag atatcgaagt ttcctccttc ggtagctcag
     2161 gtagaaactg caattcctgc ctttatcggt tatacggaaa aggcagagga tcttgcaccg
     2221 gatgatctca tcaaaaaacc gagacgcatc ggatcgctgg ttgaattttc atattattat
     2281 gggaacggcc ccgctttaaa ggtgagcgag gttgccatag atgagagcaa taatttcaaa
     2341 tcagcaaaga tcacttccga ttattatatg tatgacagtc tgaggctgtt ctttgcaaac
     2401 gggggcggcg attgtttcat agtaagtata gggaaatacc cggcagcagt tacggcaggc
     2461 gattttacag cgggcataga tacactttta aaggaggatg acccgacaat tctgctgttt
     2521 cctgatgctg tgacacttgg aacttcagat ctggctgccg ttcagacaca tgctcttaac
     2581 cattgcggcg acgaaagcag gctcgaccgt gtcacagtat ttgatctgaa agaggatgac
     2641 ccgaaaggag caaccttccg gtcaaacatt ggtaccagct tccttagcta tgggatagcc
     2701 tatactccct ggttaaagac caatctgccc aaaaaggtca ctttccccga cataaaggat
     2761 gtgataaaga aaaacggtgc atctgttacc cttaaaggat tgacaggtga tccggctatt
     2821 cagacccaga tagatgaact ggagaaggcc tatgccgata aaacaaaaat catctcggga
     2881 actgatactc ttgcaagtcc gtcagccaat ctcagggata aatacaattc agtggttgcc
     2941 acttataatg caggcaagac caccggaaat ctgcaggcag tactgactta tgtgctgtcg
     3001 attatttgga aaacagatga ttatcttaaa acaggcaatg cagcagagct gacaaataca
     3061 gctcttaaaa attcagttac tgctaatata tcaacgtttt caactgaagc aaagaaaata
     3121 atcggcctcg acaaggacct taaggctaaa tttggggcag gttataccac gcaggcgaaa
     3181 gctgccgatt ttacagatgg aatatgggga gcaggctttg ttgcaggaac cgcagcttct
     3241 ggcttgctga ccgctgcaac ggatgaagaa aacctggagt tggccctgaa tgccctgaat
     3301 aacattttcg atacgctgag cagggcatac ctctcgctca tcgtcaatgc agcagcttcc
     3361 aatgccagag atgccgatac cgctttatca cttgcattcc cgctgtataa aaatatcctt
     3421 aagggggtaa gcacttactc agcctcaata cctccgagcg gagccattgc cggaatatat
     3481 gcaatggtcg accgtacgcg cggggtgtgg aaagctcctg ccaatgtgag cctggcaaat
     3541 gtgattgagc ctacggtaac ttttaccaaa tcggagcttg atgcactgaa tgttgatgtg
     3601 aatgccggca aatcaataaa tgccataagg actttcttcg gcaagggaac acttgtttat
     3661 ggtgcccgta cacttgcagg taatgataac gaatggcgtt atgtgccggt ccgcagattg
     3721 tttatcatgg tcgaggaatc ggtcaaaaag gcaaccgaac aatttgtatt cgagcctaat
     3781 gatgccaaca catgggtgaa agtacaggct atgatcgaaa actttcttca tataatatgg
     3841 cgtcagggag ccttacaggg cgcaaaaccc gaacatgctt tttatgtggc agtcggattg
     3901 ggtaagacaa tgaatccgat tgatatcctg gaaggaagga tgataataga aataggcatg
     3961 gccgctgttc gtccggctga atttattatc atacgtttct cccataaaat ggccgagtcc
     4021 tagttatttg tatatcagag taaaataatt ttcaaatatt aaattataaa ataatggcaa
     4081 attatcctct tccgaaattc catttcatgg tagaatgggg tggcaacaag atcggattta
     4141 ccgaagtaac agggctcgac atgcaggttg aagcgataga ataccgtgaa ggttcaagca
     4201 agacatattc caaggtcaag atgcccggct tacacaagta cagtaacatt actcttaagc
     4261 ggggcacttt tgaaggagat actgatttct atacctggat caacaccata aatctcaata
     4321 ctgtggaacg cagggatata gtgatcaaac tgcttaatga aaaacacgaa ccggtagtta
     4381 cctggagggc agtcaatgcc ttcccgctga aagttcaggc cagtgacctt aaagccgatg
     4441 gcaacgaggt ggcaatcgaa agcatagaaa tcgctcacga aggcctcagt atagtgaaat
     4501 agtggctttc gcataaagat cctacaggta aaaactgcaa actatggctg acagcaaatt
     4561 accttcggca ggcggttatt atcctcctct gggatttcac tttaaagtcg aatttcaggg
     4621 aatagggaat gataatgaca cacgcttcca gtcagtttca ggcctgaata ttgaatatga
     4681 gaccgaatca ataaaagaag gcggacagaa tcattttgaa cataagctgc cggtccgttc
     4741 aaagttttcc gaccttgtgc tgaagcgcgg catgctgacc gattcggaag tgatcggatg
     4801 gtgcaaaaaa gccttattcg acagggagat cacacctgtt caggttatcg taattcttct
     4861 taatcctgaa catcagcctt taaggacctg gaaggtaaat aatgcctggc cgcgcaaatg
     4921 gaacgttagt gattttaatg ccggggagaa ctccattgtg gttgaaaccc ttgagttatg
     4981 ctatagctat tttacggtgg aataacagcg attataatgc cggttcagat tacagagttg
     5041 aatatcagtg tgtcagtaaa tcagccggga gatcagaaaa gtccgggagc acagacacag
     5101 aatcctgatg atcagggttc cgctgaaaat attgtggatg aatgtgtgga acagatcatg
     5161 aagatactga atgacaaaaa agaacgatga tggaagacag gggtgaaata aagaaaatgt
     5221 ggatcaggtc atataattcc gcaaaaatgg aggataagga ctttgcaggg gaattctatg
     5281 ccatggtcaa tcctgaaaat tacaccatcg atctgaagat ggaaacaaaa ggaggtcagg
     5341 ggcagggtac cagcggcaag caacagaagt tctcttttaa gcagcccgag gagttcacat
     5401 tcgagttttt atttgacaat accggaataa ttgacgggaa accaaagaag gcagggatag
     5461 cagacgatct gaaaaaactt caggatctgg ctttcaagct ggatggtaag gtgcatgaac
     5521 cgcgtcatat tcatctgatc tggggcacac tcgactttaa aggccgcatc acgggtatga
     5581 ccattaatta taaacttttc aatcctgatg gttccccgat ccgtgccact gtaaaagtga
     5641 caatcaagaa aagcattgaa gagaaggagc gtaccgcgga ggagcggaaa agctcacccg
     5701 accttacaca caggagaata ataagaaagg gagagtcatt gccctggttg tgcaacatta
     5761 tttacggtga tccgaaatac tatatacagg tcgcacgtgc caatcatatt tcagacttca
     5821 ggaacataaa ggagggcaca gagatatttt tcccggcatt tgctaaaaat gagtaatgaa
     5881 cgtatcatac cgtcggacca gcctaaatcg gtctgcacat tcacaatact gagtgatggc
     5941 agcgctgtgc catcagcctg gcatgtgctg agcattgtgg tatataagga ggttaatcgt
     6001 attccatcgg caacaataat tctgattgac ggtgatgttg caaccgaaac ctttgaaata
     6061 agtaactccg atgatttcaa gcccggcagg aagattgaga taaaagcggg atgggtaagt
     6121 aatgaagaga ccattttcaa aggtatggtt gttaagcaga acataaaagt aaggaaaagc
     6181 aattctgtat taatggtgga atgccgtgat gaggcgatga agatgactat cgctcctaag
     6241 agtaagtatt tcaatgacaa aaaggacagc gagatctttg aagaaataat caacgactat
     6301 ggtctgacag cggatgttga agcaaccact cttcagcaca gggaactggt ccagtacaac
     6361 agcaccgact gggatttcat gttatgccgt gccgatgtca atggaaagct tgtgattgcc
     6421 gacgacggga aaataacaat taaaaagcct gatgtaagtg caaccccgga actcacggtg
     6481 caatacggat caacagtact tgaccttgac gctgaaattg atgccaggct tcagctgccg
     6541 ggagtaaaag cagccgcctg gaactatacc gatcaggaac tgattgatac cgttgaagga
     6601 tctgaacccc ctgttcccgc tgctggtaac ctctctcccg atgatctcaa aattgcttct
     6661 tcacccggtt ctcctgtgat gatacacagc ggcaggatta ccgaggcgga attacagcaa
     6721 tgggctgatg cgcgtcttca gaaaaaccgc ctggcaaaaa taaggggaag agtaaaggtt
     6781 gaaggttatg caaagatcaa accgggtaag atactgcagc ttaacggtgt gggaacacgt
     6841 tttgaaggca ggcttatagt gtccgggata agacatcaga tcgagaatgg taactggacc
     6901 accaatatac agtttggcac cagcagcgag cggtttgcag agacttttaa tgtacagcag
     6961 cccctggcgg gtggaatgct ccctgcgatc cgcggattac agaccggaat agtgaccagg
     7021 ctggaagatg atcccgatgg agaggaccgt attaaggtcc ggctaccggt tatccataaa
     7081 agtgaagaag gtatctggtg ccgtgttgct tctcccgatg ccggaaaaga acgtggtctc
     7141 tttttccgtc ccgagatagg cgatgaagtc attgtcggct tcattgatga ggacccgagg
     7201 catgctgtgg tgcttggtat gctgaacagc agcaagctgc cggctcatac acaggcgaaa
     7261 gataccaatc acctgaaagg gtatgtatcc cgggaaaaga ttaagttgat gttcgatgat
     7321 gagaagaaag tgataacaat ggaaacaccg ggtggtaata agatcgtaat ctctgatgag
     7381 gacaaaggca taacactgac cgatcagaat ggaaataaaa tagtcatgaa caatgacggg
     7441 atatcggtgg agagtgcaaa aaacatgtcg ctgaaagctt caaatgacct gaaagctgaa
     7501 ggtctgaatg ccgagatcaa agccagcgcg cagataaaag tgaaaggaga ggccggtgcg
     7561 gaatatgctt caggcggtaa taccagcgtg aaaggatctg ttgtgaatat taactgatac
     7621 ataaatccgg attgcaatat ggcatctgca gccagagtcg gagatatgca cacatgcccg
     7681 atggtattgc cgggacctgt gccacatgtc ggagggcctg ttctgccccc gggaacgcca
     7741 actgtgatga tcgccggtat gccggctgct accgtaggtg atatgtgtac ctgcaccggt
     7801 cctccggatt caatagtcaa gggatcggca actgtgatga taggaggaaa accggccgcc
     7861 cgtatgggtg acagcactgc acacggagga gttattatcg ccggagcccc cactgttaat
     7921 ataggaggtt gaaattatgg atgaatcatc agatgacagg aatttcctgg gtagaggctg
     7981 gagtttcccc cctcagttcg accctgtgtc caaaaccgta attatgactg aggaggctga
     8041 ggatattaat aacagtcttt caatactatt gtcgacaata gtgggtgaac gcgtcatgga
     8101 tatgagctat ggctgtaatc tggaggatat ggtatttgaa actctcgata catcagtgat
     8161 aacttatctc aaaagcaaaa taacgaaggc tattttgttc tatgagg
//
LOCUS       DAOSPX010000129         8188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8278_length_8188_cov_4.342555, whole genome shotgun sequence.
ACCESSION   DAOSPX010000129 DAOSPX010000000
VERSION     DAOSPX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8278_length_8188_cov_4.342555"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..1686
                     /locus_tag="PLZ75_07600"
     CDS             331..1686
                     /locus_tag="PLZ75_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS72296.1"
                     /translation="MKTTDVLVIGGSAAGMVAALTGKSSWPGKSFTLVKKQKDMMVPC
                     GIPYIFGTLSSVSQNIMPVDTMMEKAGIESIVDEATFISTENKYVDFASGERIKYDKL
                     VIATGSTPVKPAWLTGGDKENVYVIPKDKVYLEDMKTRLASQKKVVVIGAGFIGVEFA
                     DELVKSGHDVLLVEKLPDILMLAFDAELSKKIEEILIQRGVKVITGNGIKEIHGGSKV
                     EKVELENGEFFDADAVVLSIGYKPNVELARKSGIYVDESNFIATDEYMRTHIKDIFAV
                     GDCAQKRDFVTRARVSTMLASTACAEGRIAGMNLFNLNVVKTFSGTIAIYSTAIGDTG
                     FGTAGVTEARAKAEGIDTVTGVFEGIDRHPGNMPGAMKQIVKLIVARHSGVIIGGEVI
                     GGLSAGELTNVIGLAIQNRMTINSLLTSQIGTHPCLTASPAGYPLIKAAEIIAGKIIG
                     R"
     gene            complement(1704..2741)
                     /locus_tag="PLZ75_07605"
     CDS             complement(1704..2741)
                     /locus_tag="PLZ75_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72297.1"
                     /translation="MQKSKYFRFLISFVAGIRFRLIILLISGAFAAGHLSGQQTGDFK
                     PGGRPEARIFTSFSSTFEDGENHNKFDIARAYLGYSYNFSQKFSGRIVYDVADPSAGN
                     LKFTGMLKFAYLRYQTDRWTITGGMIPLPEYDQGDRKWGFRYVYKTLHDEYGFGTSAD
                     LGLSIGYKISPLLSADLTIMNGEGFKLTETDSILKGAAGITLTPAEKFSLRAYYDVMA
                     GNKGNQHTAEFIAAYESKELSLSGAYNFRKNNNLREGHNYHGLSVNGAVSLSDRIRLF
                     GRFDHVASVTALGGLDSWNLKKAGQLVIAGMEIGLAPGVSIAPNFQGWSAAGSGMPFV
                     SRFSLSLDLKL"
     gene            3052..4350
                     /locus_tag="PLZ75_07610"
     CDS             3052..4350
                     /locus_tag="PLZ75_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOS72298.1"
                     /translation="MTEGKKSDTAAKYNIGQDWASVVTGFILIIFVIATGYSVAIPSF
                     GGKAGWTASGGIAGMFEGISGMAIIYTILVFGLISALGMFLSGDSLKKFLTGFLTVFL
                     LALIAQFISSYSFFKTLGLETVFFSLLLGLLIGNFFRLPEWVKQGVQTELYVKIGLIL
                     LGATILFKDILTAGTFGLIQAVIVVISVWYFAFWISRKLKVDDEFSTMLASAVSICGV
                     SAAIATAGAINGDKKKLSFTISLVLIVAIPMMIFLPVIAKWMGLSDIVTGAWLGGTID
                     TTGAVVAAGTITGETGLKYATIVKFSQNVLLGLAAFIISIFWAYRNKDGRNNENRVPV
                     SVIWERFPKFVLGFILASLLFSFILSPSTAASSGKVMKAFSGFWFNLAFISIGLETRF
                     ADFKQMDSKKPFYSFLIAQGFNIIFTLGVSLILFQILKPA"
     gene            complement(4372..5775)
                     /locus_tag="PLZ75_07615"
     CDS             complement(4372..5775)
                     /locus_tag="PLZ75_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOS72299.1"
                     /translation="MKQFDDLFRENVKKVFGSYNADHLAGEGWNSFVSARKARSRRMT
                     VIPVLARAASVLLIVGLGAFIAYRISTRKSISVSESVVQQDRLMDAGDIERTGTGRSS
                     GVPEPSEITEAKAAKETAASAASGKPVEYPVSGLSLAGKTTVTGTDAFAGVTAIAGNA
                     AVSPAAHAAETVYGESRTGMKAAESYFLIPHVLADLHSSGVRKESDDKTGTGELIPSV
                     TVSTGITSDGPGVAAKTSGQEARHGGRALIAGFSGLLARSGGSASPSSGLSVGLYLDQ
                     KLTKKISVRSGLALAMQSFELMNGNDFIKTSGLFNDPLSLSDGTSGKPYSYDGKLNML
                     AMELPLNIVFRISDRKGSGFFVSAGASTMIYLSQQFTADFVNEYTRLALNAETGVYST
                     ESRFSTLGVEKDYSAFSRTDFFGLANLSAGYNFNYSKTGTMLIEPFLQLPVSDLTSFN
                     LRIRYGGLSMKLRFGNP"
     gene            complement(5772..6350)
                     /locus_tag="PLZ75_07620"
     CDS             complement(5772..6350)
                     /locus_tag="PLZ75_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS72300.1"
                     /translation="MRRREKDDHTISDPELIRRCSEGDQRAQELLYRRYFSFAMSVCV
                     RYTGDQYDAMEVVNDSYMKVLGSIKDIDSSKSFRSWYGRILVNSAIDRYRRNTRHVSH
                     LQLNDLETADGQEDPVSTELSANEILKLFDRLPDNYRVTFNLYEIEGYSHEEIGQMLD
                     ISASTSRSNLFRAKRMLRMLYNRNFNTEKRGE"
     gene            complement(6441..7187)
                     /locus_tag="PLZ75_07625"
     CDS             complement(6441..7187)
                     /locus_tag="PLZ75_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72301.1"
                     /translation="MNDTGIAAVIILFSLSSGLAAQKQEKQTIVLNGGTLITGIILAD
                     SPDYLTVKIRSPRVIRINKSEISPGTPALEIERPFSGRQGYAIGLSASVLAGRNSEGN
                     IRDMSFLVSNSYRFRKGLSIGFGTGIEEFDVSLIPVYSELKYYFFRSRLSPYIWTKSG
                     WAFAYDDRKDGEYNHYYNYYPEVKGGFMFNAGSGLELASWRRNAVNMGVGFRFQRLIY
                     SNMTGRGKENLTELVTSIKRIEVQFGFIFR"
     gene            complement(7191..>8188)
                     /locus_tag="PLZ75_07630"
     CDS             complement(7191..>8188)
                     /locus_tag="PLZ75_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72302.1"
                     /translation="GNVDMAVRGNILFADSFIDLLAIDITDVEKPVEISRIENVFPEV
                     MPQGDEWLPYAMVDKSKGVIVGWKTETITEEYEPGSRWTGWIFRGRMTLMANYGSDAA
                     GWSAGAGTAGSMARFMLNDKYLHVIAHPWMLKTVDVENAKKMVIADSVEVSRDMETLF
                     LLGGRMFVGTTTGMLIYDVTNATKPVLISHYDHITACDPVVADENYAYVTLRSGSRCR
                     NTQNLLEVIDISSIEHPYLVKSYPMYNPHGLGIDDNLLFICDGAAGLKIYDRSDPLDI
                     INRKVAHYPDFFTYDVIPMKGVLMLVGEKGIYQYDYSDPENIVQISHISISSGSK"
BASE COUNT         2201 a         1960 c         1825 g         2202 t
ORIGIN      
        1 gctcggaaga gagctgctca gaaacccgta tttcccgctg aaaacagccg gaagctggaa
       61 ggaggaaatc agctggcaga aacaatatct ccgggcaaaa taaataccgg tatccgggaa
      121 agaaaagaaa attaatttgg agaatgaaca tatgttcatt ttatttgcat aagaatttca
      181 gcaacattaa aatctgttac ggcagggaag catctcaatg caaatattcc tgaaacacaa
      241 ggaatcaggt caggaaaaaa cctgcagccg gctttcaggc agaatattca gctgaacgtt
      301 caggtttaac aaattaaata agatatagaa atgaaaacaa cagatgtatt agtgattgga
      361 ggaagtgcag cagggatggt tgctgccctt acgggaaaat catcatggcc gggaaagagc
      421 ttcactctcg tgaagaaaca gaaggatatg atggtaccct gcggaatacc ctatatattc
      481 ggcaccctca gcagtgtaag ccagaatata atgccggttg acacgatgat ggagaaagcc
      541 gggattgaat ccattgtgga tgaagcaaca ttcatcagta ctgaaaacaa atatgttgat
      601 tttgccagcg gtgaaaggat aaaatatgac aagcttgtca ttgccacggg ttcaacaccg
      661 gtgaaacctg catggctaac ggggggcgat aaggaaaatg tgtacgtcat accgaaagac
      721 aaggtttatc tcgaggatat gaaaaccagg ctggcttccc agaagaaggt tgtggtgatc
      781 ggggcaggct tcataggtgt tgaatttgcc gatgaacttg taaaaagcgg ccatgacgtc
      841 ctgctggtgg aaaaacttcc tgatatcctg atgcttgcct ttgatgcgga attatcaaag
      901 aaaatcgagg agatactgat tcaaagaggc gttaaagtca ttacaggcaa tgggattaaa
      961 gagattcacg gaggcagcaa ggtggaaaag gtggaacttg aaaacggcga attttttgat
     1021 gccgacgctg tggttctctc aatcggctac aagccaaatg ttgaacttgc acgcaaatcg
     1081 ggaatatatg ttgacgaaag taattttatt gccaccgatg aatacatgcg gacacatata
     1141 aaggacattt ttgccgtggg cgactgtgca cagaagcggg attttgtcac acgcgcaagg
     1201 gtgtcaacca tgctggcctc aaccgcatgt gccgaaggac gtatcgcagg aatgaacctg
     1261 tttaacctca atgtggtgaa aaccttcagt ggcacaattg caatctattc cactgcaata
     1321 ggcgatacag gcttcggaac ggcaggagtt accgaagcaa gggcaaaagc cgagggaata
     1381 gacacggtaa ccggtgtctt tgaaggcatt gaccgtcacc cgggaaacat gcccggagcc
     1441 atgaaacaaa tcgtaaaact gattgtcgcg cgccattccg gtgtcattat cgggggtgaa
     1501 gtcatcggcg gactgagtgc gggggaactg acaaacgtta ttggtctcgc tattcagaac
     1561 cgtatgacga taaattcact gcttacttcc cagatcggaa ctcacccctg cctgaccgcc
     1621 tcaccggcag gctatcccct gatcaaggca gcagaaataa ttgcaggaaa aataatagga
     1681 agataaaacc ggacctcccg cagctagagc ttcagatcaa gcgataaaga aaatctggag
     1741 acaaaaggca tgcctgaacc ggccgcgctc catccctgaa aattcggagc aatactgaca
     1801 ccgggagcca gtccgatttc cataccggca ataacaagct gtccggcctt tttcaggttc
     1861 caggaatcca gtccgccgag ggctgtaacg gaagcaacat gatcaaacct tccgaacagg
     1921 cgtatcctgt cactcaggga tacagctccg ttaaccgaaa ggccatggta attatgccct
     1981 tccctcagat tattgttctt cctgaaatta tatgctcccg acaggctcaa ctctttactt
     2041 tcataggctg cgataaactc tgcagtatgc tgattgcctt tgttccctgc cataacatca
     2101 taataagccc gaagtgaaaa cttttccgcc ggcgtaaggg ttatgccggc cgcacctttc
     2161 aggatagaat cagtttcagt gagcttaaaa ccctcgccat tcattattgt cagatcagcc
     2221 gacagcaatg gggatatttt gtagccaata ctcagtccga gatcagctga agtaccgaaa
     2281 ccatattcat catgcagggt tttgtacacg tagcggaaac cccacttcct gtcgccctga
     2341 tcatactccg gcaggggtat catgccgcct gttatggtcc atctgtctgt ctggtaccgc
     2401 agatatgcaa atttcagcat gcctgtaaat ttcagattac ctgccgacgg atccgcaaca
     2461 tcatagacta tccttccgct gaacttctgc gagaaattat aactgtaacc cagataggct
     2521 cgggcaatat caaacttatt gtgattttcg ccgtcttcaa aggtactgct gaaacttgta
     2581 aagatccttg cctccggtct gcctcccggt ttgaaatctc ccgtctgctg tcccgacaaa
     2641 tggcctgcgg caaatgcccc gctgatcagc aggatgataa gccggaacct gataccggca
     2701 acaaatgata tcagaaatct gaaatattta cttttctgca taaaagcttg ttttttgttt
     2761 ctaaaaattc acgctgccgg attctgaata ccgtcccgga atttaggggc ttaaattaag
     2821 taaagattat ttcgctgatg ccggctcctt ttgatggcag gacaatgaat aaaaaccgct
     2881 ataataaaaa tcacgaattc aacttattga ttttaaatat gctgtatttg aaattaagaa
     2941 atgcggcttc ggaaaaggcg tatataaaaa tacgatttta tgcccactaa ataatttaca
     3001 acaggtttaa tctttgtggg gataattaaa tcctaacaaa aatatcacaa aatgactgaa
     3061 gggaaaaaat ctgatactgc cgctaaatat aatatcggac aggactgggc atccgttgta
     3121 accggtttta ttctgataat ttttgtaatt gcaaccggat actctgtcgc cataccctcc
     3181 tttggaggca aggcgggctg gacagcctcc ggcggcattg ccgggatgtt tgaaggaata
     3241 tccggaatgg ccataattta tactattctg gtttttggac tgatctcagc actgggtatg
     3301 ttcctcagcg gtgattcact gaaaaaattc ctcaccggat tcctgacggt ttttttgctt
     3361 gcattgatag cacaattcat ttcatcatat tcatttttca agaccctggg acttgaaaca
     3421 gtttttttct ccctgcttct cggattgctt atcggtaact tcttcaggct gccggaatgg
     3481 gtgaaacagg gcgtgcagac agagctttat gttaagatag gacttatcct gctcggggca
     3541 acaatattgt tcaaggacat tctgactgca ggaacattcg ggctcattca ggcagtaatt
     3601 gttgttatca gcgtatggta cttcgctttc tggatttccc gcaagctgaa ggttgacgat
     3661 gaattttcga ccatgctggc cagtgcggtt tcaatctgcg gtgtgtcagc agcaattgcc
     3721 accgcaggag ccatcaacgg agacaagaaa aaactttctt tcaccatttc cctggtgctg
     3781 atagttgcca ttccgatgat gatattcctt cccgtcattg caaaatggat gggactttcc
     3841 gatattgtta ccggtgcatg gctcggaggt accatcgata ccaccggggc ggtggtggct
     3901 gccggtacca ttacagggga aacaggactg aaatatgcta caatagtcaa gttttcccag
     3961 aatgtgctgc tgggcctggc tgcattcatt atctcaatat tctgggcata caggaacaag
     4021 gatggcagga ataatgaaaa ccgtgttccc gtaagcgtta tttgggaacg ctttccgaaa
     4081 tttgtactcg gcttcatcct ggcttccctg ctgttctcat tcatactgag cccgtcaaca
     4141 gcagcatcct caggaaaggt aatgaaggca ttcagcggat tctggttcaa cctggccttt
     4201 atcagcatcg gtctggaaac ccggtttgct gatttcaagc aaatggacag caagaagcct
     4261 ttttacagtt tcctcattgc ccagggattc aatattattt ttacccttgg agtatcactt
     4321 atcctgtttc agatactgaa acccgcatag tgcggcgata cctgaagaga atcacggatt
     4381 tccaaaccgg agtttcattg acaggcctcc atacctgatc ctgagattaa aacttgtaag
     4441 gtcacttaca ggaagctgaa ggaagggctc aatgagcatt gttccggttt tactgtaatt
     4501 aaaattgtat ccggctgaaa ggttggccag accgaaaaag tcggtacggc tgaatgcact
     4561 gtaatccttt tcaacaccaa gggttgagaa acgggattcg gttgaataaa caccggtttc
     4621 ggcattcagg gcaagcctgg tgtattcatt tacaaaatcg gctgtaaact gctgactgag
     4681 atatatcatg gtagatgccc ctgcggaaac aaaaaagccc gaacccttcc tgtcgctgat
     4741 cctgaataca atattcaaag gtaattccat tgcaagcata ttcagctttc cgtcatatga
     4801 gtaaggtttg ccgcttgttc cgtcagaaag cgacaggggg tcattgaaca atcctgaggt
     4861 tttgatgaaa tcattgccgt tcatgagttc gaatgactgc atggcaagtg ctagtcccga
     4921 ccgtaccgag atctttttcg taagcttctg atcgagatag agcccgacgg agagtcctga
     4981 agagggggat gccgacccgc cgctccgggc aagcaatcct gaaaaacccg caatcagtgc
     5041 ccttcctcca tggcgcgcct cctgtccgga tgtctttgct gcaactcccg gaccatcaga
     5101 tgtaataccc gttgataccg taaccgaggg tattaattct ccggtaccgg ttttatcatc
     5161 tgattccttt ctgacccctg agctgtgaag atctgccagt acatgcggaa tcaaaaaata
     5221 gctttcggcc gctttcatcc cggtcctgct ttcaccgtaa acggtttccg cagcatgtgc
     5281 ggccggtgaa acagctgcat ttcctgctat ggcagtgact cctgcaaagg cgtcagtccc
     5341 cgtaacagta gtttttcccg cgaggctcag gccggataca ggatattcaa caggttttcc
     5401 agatgctgcg gatgctgccg tttccttagc tgctttcgcc tctgttattt ctgatggctc
     5461 cggcacaccg gaagatctcc ctgttcccgt cctttcaata tcgccggcat ccatcagccg
     5521 gtcttgctgt acaactgatt cagacactga aatactcttc ctggtggata ttctgtaagc
     5581 aatgaaagct cccagaccca caatcaaaag aactgaagct gcccgtgcca gcaccggaat
     5641 cactgtcata cgcctgctcc gggctttcct ggcggaaaca aaggaattcc acccctctcc
     5701 ggcgaggtga tcggcattgt aactgccaaa aactttcttt acattttccc tgaacaggtc
     5761 gtcaaactgt ttcattcccc tcgtttttca gtattgaaat tcctgttata caacattctg
     5821 agcattcttt ttgcccggaa aagattcgat cttgaagttg atgcactgat atcgagcatc
     5881 tgtcctattt cctcatggga atagccttca atttcataaa ggttgaaggt aaccctgtaa
     5941 ttatccggca gccggtcaaa cagtttcagg atttcatttg cggaaagttc agtactgacc
     6001 gggtcttcct gcccatccgc cgtctcaagg tcatttagct gcagatgcga aacatgtctt
     6061 gtatttctcc tgtatctgtc aattgccgaa ttgaccagta tccttccgta ccacgacctg
     6121 aaagatttcg aactgtcgat atcctttatg ctgccaagca ccttcatgta actgtcattt
     6181 actacttcca tggcatcgta ctgatctccc gtatacctga cacataccga cattgcgaat
     6241 gagaaatagc gtctgtagag aagttcctga gcacgctgat ctccctcgga acaccgcctg
     6301 ataagttccg gatcggatat tgtatggtca tccttctctc tccttctcat tacctgcatt
     6361 cagattaagc ttcatcaaaa taacttaatt tgaatggttt tatgacatta ttatccataa
     6421 ttctgtaacc gtatgctgtt ttacctgaat ataaaaccga actgtacttc aatccgttta
     6481 atactggtaa caagttcagt aaggttttct tttccccttc cggtcatatt gctgtaaata
     6541 agtctctgaa accggaagcc tacacccata ttcactgcat ttctccgcca tgaagccagt
     6601 tcgagtccgg atcctgcatt gaacatgaat ccgcccttca cctcaggata gtaattatag
     6661 tagtgattat actccccgtc cttcctgtca tcataagcaa atgcccatcc gctctttgtc
     6721 catatatacg gcgacagccg ggatctgaag aaataatatt tcagttcgga atatacaggt
     6781 atgagagaca cgtcaaattc ctcgatccct gttccgaaac cgatactcaa gcccttacgg
     6841 aaccggtagg agtttgacac cagaaagctc atgtccctga tgtttccttc actgttgcgg
     6901 cccgccagta ccgaggctga aagtccgatc gcatatccct gcctgcctga aaagggtcgt
     6961 tcaatttcca gcgccggagt gcccggcgat atttctgatt tattgatcct tataaccctg
     7021 ggggacctta ttttcactgt cagataatcc ggggaatcgg ccaggatgat gccggtaatc
     7081 aatgttccgc cattcagcac gattgtctgt ttctcctgtt tctgggcagc aagaccgctg
     7141 ctcagcgaaa aaaggataat aaccgctgcg attcctgtgt cgttcatatt ttatttactg
     7201 ccgctgctga ttgatatatg gcttatctgc acaatgtttt caggatcgga ataatcatac
     7261 tgatatattc ctttttcccc tacaagcatg agaacccctt tcattggtat gacgtcatag
     7321 gtaaagaaat caggataatg agccactttc ctgtttataa tgtcaagcgg atcggaacgg
     7381 tcataaatct tcagtccggc tgcaccatcg cagataaaga gaagattatc gtcaataccc
     7441 agtccgtgcg gattgtacat gggataggat tttaccaggt agggatgttc aattgatgaa
     7501 atatcaataa cctcgagaag attttgtgtg tttctgcacc tgcttccgga acggagggtt
     7561 acgtatgcat aattctcatc agccaccacc gggtcgcatg cagtgatatg gtcataatgt
     7621 gaaatcagca cgggctttgt ggcattcgtg acatcataaa tgagcatgcc ggtggttgtc
     7681 ccgacaaaca ttcttccccc gagcaggaaa agagtttcca tatcacggga tacttccaca
     7741 ctgtcggcaa taaccatttt ctttgcattt tcaacatcaa cggtcttcag catccaggga
     7801 tgggcaatga catgcagata tttgtcattc agcataaacc ttgccataga gcctgctgtt
     7861 ccggcaccgg ccgaccagcc ggcagcatca cttccgtagt tagccatcag cgtcattctt
     7921 cccctgaata tccaccctgt ccatctgctg ccgggctcat attcttcagt tattgtttcg
     7981 gttttccatc cgactatcac acccttcgac ttatcaacca tggcatatgg cagccattca
     8041 tccccctgcg gcataacttc cggaaaaaca ttctcaatcc tgctgatctc aaccggtttt
     8101 tccacgtctg ttatatcaat tgccagcaga tcgatgaaac tgtctgcaaa aaggatatta
     8161 cccctgaccg ccatgtctac atttccgg
//
LOCUS       DAOSPX010000130         8180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8289_length_8180_cov_3.883938, whole genome shotgun sequence.
ACCESSION   DAOSPX010000130 DAOSPX010000000
VERSION     DAOSPX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8289_length_8180_cov_3.883938"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLZ75_07635"
     CDS             <1..342
                     /locus_tag="PLZ75_07635"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOS72303.1"
                     /translation="LVYALLIIPRLVFPQTIVIGRRKTQITMIAAVIELGLNIPLSLL
                     MIKWGYGIVGVALATFIVYCVSKIYLAAYLWVKLKIKPSEYIPLKAFLVYSLLTTVVF
                     LLVDRRIIDIQ"
     gene            675..1877
                     /locus_tag="PLZ75_07640"
     CDS             675..1877
                     /locus_tag="PLZ75_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72304.1"
                     /translation="MKITEVVSKHERREFIEFPRQLYHDDPLWVCPLDSMIESVFDPL
                     RNYSFRHGEATRWLLRDSGGRVTGRIAAFIDRQRSAAQAQPTGGIGFFEVIDDQESAF
                     MLFDTAREWLTARGMEAIDGPVNFGENDNNWGLLVEGFEQPVFGMPYNKRYYRDFFEA
                     YGFRNYFEQYSQSRELRNSEGTIIEFPERIMKIAGRISERPGYSFRHFEMKNSHKFSS
                     DMCEIYNSTWVFLKEDFTPLEPDVFNESLRQARMIIDEELIWFAYHNEKPIGFFILLP
                     DLNQILRHFNGRLTPWNMIRFLYYKMTNEIDRARAIVGGIHHAYQNSGIESAMFYHFY
                     QAFKKRKWLKSLEFAWVGDYNPKMLALYNALDTVRTKTHITYRYLINDKLRFVRYKDE
                     KEMAEKKP"
     gene            2123..2386
                     /locus_tag="PLZ75_07645"
     CDS             2123..2386
                     /locus_tag="PLZ75_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOS72305.1"
                     /translation="MKQGIHPENYRFVVFQDMSNGHSFLSKSTAPSKETIKWEDGNEY
                     PLIKLEISNTSHPFYTGKMKLVDTAGRVDKYMNRYRDHVNKKK"
     gene            2727..5141
                     /gene="lon"
                     /locus_tag="PLZ75_07650"
     CDS             2727..5141
                     /gene="lon"
                     /locus_tag="PLZ75_07650"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOS72306.1"
                     /translation="MIDGEGDIIPIISDGDDRELEDVEVPETLPVLSLRNTVLFPGVV
                     LPISIGRPRSVQLIKDVYRTDKIVGTVAQKDPDMDNPGFEDLHQVGTIGQIVKLLEMP
                     DGSTTAIIQGRKRMVLSELVSDNPYFIARVKAIPEKRPDKRSKDFDAIVGSLRDLSLK
                     IIKINPNISPEASFAIKNIENNTYLINFICSNTDIKVQDKQKLLEINNIRDRGFMLLE
                     FLVQEIQILELKNELQSKVKSDLDQQQREFLLHQQMKTIQNELGGNPAEKEIEEYIRK
                     AADKKWSSEVRQVFDKELDKLRRLNPAAAEYSVQINYIQTLLDLPWGEYTQDNLDLQN
                     ARQVLDNDHFGLDDVKERILEHLAVLKLKGDLKSPILCLHGPPGVGKTSLGKSVARAL
                     GRKYVRMSLGGLHDEAEIRGHRKTYIGAMPGRIIQNIKRAGSSNPVFILDEIDKVGQD
                     FRGDPSSALLEVLDPEQNSSFFDNFLEMEYDLSRVLFIATANTLTTISPALRDRMETI
                     EITGYLVEEKLEIASRHLLPRQLENHGVKADQLEIGKDVLEKLIDSYTRESGVRELDK
                     RLAKIVRVTAKNIASETKYNHDLTEKSLEKILGPAKYNRDLYSGNDQAGVVTGLAWTP
                     AGGEILFVETSISKGSGKLTLTGNLGEVMKESAILALEYLKANAGLLELPDNFSEKWN
                     VHLHVPEGAIPKDGPSAGIAMATSIASAFTQRKVRKYLAMTGEITLRGKVLPVGGIKE
                     KILAAKRANIREIVLSEENRKDVGAIKDIYIKGLKFNYVDSIMDVLDLALLRQKVSNA
                     RKIVFE"
     gene            5138..5617
                     /gene="coaD"
                     /locus_tag="PLZ75_07655"
     CDS             5138..5617
                     /gene="coaD"
                     /locus_tag="PLZ75_07655"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOS72307.1"
                     /translation="MKKIAVFPGSFDPFTLGHEGIVLRGLSLFDEIIISVGANALKKN
                     YYSLETRKAMISEVFRNEPRIKVDHYEGLTVDYCKRHGARYILRGLRTAADFEFERAI
                     GQVNKTLSPEIESVFLLTVPEHTMINSSIIRDIIRSGGDASGFVPKAIKLEEYRGED"
     gene            complement(5614..6225)
                     /locus_tag="PLZ75_07660"
     CDS             complement(5614..6225)
                     /locus_tag="PLZ75_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOS72308.1"
                     /translation="MYKVHFENRFILISPEPDRLQKYGLFVKFNDTDELYRIINDFQT
                     DTEMQSINIYGPDIRHIWKIFRIYFSEVGAAGGLVKHSSGRYLFIEKKGRLDLPKGHI
                     EPGEKPETCAVREVSEEAGIKGHSIIKSLTPTYHTYTLKGISYLKKTSWFLMKYDGEM
                     VTEPQAEEGITRVEWLLPDELSIIKSTAWLSLMDMINTSVLKF"
     gene            6232..6867
                     /gene="pyrE"
                     /locus_tag="PLZ75_07665"
     CDS             6232..6867
                     /gene="pyrE"
                     /locus_tag="PLZ75_07665"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOS72309.1"
                     /translation="MDTYSKKIAEYLLQIKAIKLQPSNPLTWVSGWKSPVYCDNRKTL
                     SFPKVRKYIRDAFVTVINEHYPDAELIAGVATGAISHGALVADRLELPFVYVRSGAKT
                     HGLGNQIEGFYEPGQKTVVIEDLISTGGSSLNAVRTLRDAGCEVLGLAAIFTYEFRKS
                     EDAFIAEKCRLDTLTNYTVLIQTALETGHITEDMMDTLRDWRLDPENWGRN"
     gene            6876..7322
                     /locus_tag="PLZ75_07670"
     CDS             6876..7322
                     /locus_tag="PLZ75_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72310.1"
                     /translation="MASPSRFESRIAKISYSPETVYNFASDMRNFRRFVPSGTFSNPD
                     FRQDSCSFIVNPVGTVKVFIKDRVLSNKIVYSGNALLQNDFDMILDIRNSAGGHSEIK
                     LSVEAELNPFLLMLAKEPLDKFLETIMDEMEKFEGWNDVSADTESP"
     gene            complement(7297..8037)
                     /locus_tag="PLZ75_07675"
     CDS             complement(7297..8037)
                     /locus_tag="PLZ75_07675"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOS72311.1"
                     /translation="MIIGSDILYFDSLASTNVTAAQMLTEQKPEEGTVIRAGFQTAGK
                     GQKGNSWESAEDMNLLFSIILYPSSLQADEQFLLSMAISLGICDFLENHLPRPDIKWP
                     NDIYAGGKKIAGILIENSILGEMIEYSVVGTGININQKKFSPGISGAVSLGIITGKEY
                     DTGSLFRQVLHFLDLRYKQLLYGDRDAIREEYFSNLYRAGEWHNYSSDGVVFRGAIRG
                     VSPSGILTVEKEGNEYCEFSFREIQYLH"
BASE COUNT         2388 a         1701 c         1995 g         2096 t
ORIGIN      
        1 cttgtatatg ccctccttat tattccgcga ctcgtttttc cgcaaaccat cgtgattggc
       61 aggagaaaaa cacagatcac catgattgcc gccgtgatag aacttggact gaacataccc
      121 ctaagcctgc ttatgataaa atggggatac ggcattgtag gtgtggcact ggccacgttt
      181 attgtgtatt gcgtgtcaaa aatctacctg gcggcctatt tgtgggttaa attgaaaatc
      241 aagccatcag agtatattcc ccttaaagct ttcctggtct attcactgct gacgacagtg
      301 gtgttcctcc tggtagaccg tcggattata gatattcaat aattagatat tcaatagatt
      361 gtgcaggaag cagtttttaa tttatgcttt gtgaagggga aaggtttttt attatcatta
      421 tttttacgtt attaaacagg gaaaggagca gttatttttg caagccgggg aaaccggtcc
      481 ggcagatggc agcggatccg gtatacaggg aatccggcag cctggcggtg gatggaagaa
      541 caaccggcgg acaggcagct cggacataca ggcaacgaag agggaaacag tgaattttcc
      601 ggaagaggat aggaaagagg ccggacggaa aatgagaaaa gactaaagaa gagtactgta
      661 aaagagatat ctgcatgaaa attacagagg ttgtcagcaa gcatgaacgc agggagttta
      721 ttgaattccc caggcagttg taccatgatg atcccttatg ggtttgtcct ctggattcaa
      781 tgattgaatc ggtatttgat cccctcagga attattcctt caggcacggg gaggcaacgc
      841 gatggctgct cagggactcc ggaggcaggg ttacgggacg gatagctgct tttatcgaca
      901 ggcagagatc agccgcccag gctcagccca ccgggggaat aggatttttc gaagttattg
      961 atgatcagga atcagccttt atgctttttg acacagccag ggaatggctt accgcccggg
     1021 gaatggaggc aattgacggt cctgttaatt tcggggaaaa tgacaataac tgggggctgc
     1081 tggttgaagg ttttgaacag cctgtgttcg gtatgccata taataaaagg tattacaggg
     1141 acttttttga ggcttacgga ttcagaaact attttgaaca gtattcacag tccagggaac
     1201 tgcgaaactc tgaaggaacg attattgagt ttcctgaaag gataatgaaa attgccggca
     1261 ggatcagcga gcgtccgggc tacagtttca ggcattttga aatgaagaac agtcataagt
     1321 tcagcagcga catgtgtgag atatacaact ccacatgggt gtttctcaag gaagatttta
     1381 cgccgcttga accagatgtt ttcaatgaat cgctcagaca ggcaaggatg ataattgatg
     1441 aagaactcat ctggtttgcc tatcataatg aaaagccaat aggattcttc atactgctgc
     1501 ccgatcttaa ccagatactg cgtcatttta acggcaggct tacaccatgg aacatgatac
     1561 ggttccttta ctataaaatg accaatgaga tagacagggc ccgggcaatt gtcggaggaa
     1621 ttcaccatgc ataccagaat tccggaattg aatcggcaat gttttaccac ttctatcagg
     1681 cctttaaaaa gagaaaatgg ctgaaaagcc ttgaatttgc atgggtagga gactataatc
     1741 ccaaaatgct ggctctttac aacgcccttg acacggtaag gacaaagacc cacatcacat
     1801 accgttatct cataaatgac aagctgcggt ttgtcaggta taaggatgag aaggagatgg
     1861 cggaaaaaaa gccctgacgg gatggaagtc agtaaggtaa caggagaagg ccggagagac
     1921 cggaagtata agtgaggaac caggtaagca gaaaagccgg ggaagagagg aattccctcc
     1981 ggaattatgt gtcaggttgt cagccggaaa tttcacatga gtattttttt ttgtgattat
     2041 tttgccggtt gaaaatatct gattaatttt gcacgccccg cttaggtgaa attatttaat
     2101 acagagaata aacagtacag agatgaaaca gggaatacat ccggagaatt acaggtttgt
     2161 ggtctttcag gatatgtcaa acggacattc ctttctgagc aagtccacgg ctccttcaaa
     2221 ggaaaccatt aaatgggaag acgggaacga ataccccctt atcaagctgg agatatcaaa
     2281 tacttcccat cccttctata ccggaaagat gaaactggtg gacactgccg gaagggttga
     2341 caagtacatg aaccgttaca gggatcatgt gaacaagaaa aaatgaagca ctcttaaatc
     2401 catgatatgt taagggctct gtacaggggc cctttttttt gtgcaaggtt tattttatca
     2461 cagccttaca attattctgc ctgcctggtg gaaaaacagt gaaatatgcc tctttcgcaa
     2521 aaacgcctgt atctgtctga cagaaagcag gaaataaatg tttctttggt cttgaaacat
     2581 ttggcataat gattgcgtag tacagaaacg gcttttttga gaaaattatc agtaattttg
     2641 gcattttccc ggaaagcagt gtttcttaaa caagataaat ggaaaagaaa ttccgattca
     2701 cgataaatga tctttttctc ccggatatga ttgatggtga gggagacatt attcccataa
     2761 tatcggatgg cgacgacagg gaacttgaag atgttgaagt tcctgaaacc ctgccggtat
     2821 tgtcactgag aaatactgtg cttttcccgg gagttgtact gccaatatca atcggaaggc
     2881 cccgttccgt acagcttatc aaggatgttt accgcaccga taaaatagtg ggaactgttg
     2941 cccagaaaga tccggatatg gataatcccg gatttgaaga cctgcaccag gtagggacaa
     3001 tagggcagat agtgaagctt ctcgagatgc ccgacggatc aaccactgcc attatacagg
     3061 gcaggaaaag aatggttctg agtgagcttg tctcggataa cccgtatttc attgccaggg
     3121 taaaggcaat tccggaaaag aggccggaca agcgaagcaa ggactttgat gcaatagtag
     3181 gttccctcag ggacctttcg ctcaagatta taaaaatcaa tccgaatatc agtcccgaag
     3241 caagtttcgc aataaaaaat attgagaaca atacctatct gatcaacttt atctgttcga
     3301 atactgacat aaaggtccag gacaagcaga aacttctgga gataaacaac atacgcgaca
     3361 ggggctttat gctgcttgaa ttcctggtac aggaaatcca gatccttgaa ctgaaaaatg
     3421 agttacagtc aaaggtaaag agtgatcttg accagcagca gagggaattt cttcttcatc
     3481 agcagatgaa gaccatccag aatgagcttg gaggaaatcc ggctgaaaag gaaatagagg
     3541 agtacatcag aaaggctgct gacaagaaat ggagctctga agtcaggcag gtgttcgaca
     3601 aggaacttga caagctccgc aggctgaatc ctgctgcagc cgagtattcg gtgcagataa
     3661 actacataca gacccttctc gacctgccct ggggtgaata cacccaggac aatctggatc
     3721 ttcagaacgc aagacaggta ctcgacaatg atcatttcgg acttgatgat gtaaaggaac
     3781 ggatacttga acatctggca gtgctcaaac tgaagggtga tcttaaatcg ccgattctct
     3841 gcctgcacgg cccgcccggg gttggcaaga cttctctcgg aaaatccgtt gccagggccc
     3901 tgggaagaaa atatgtcaga atgtcacttg gaggacttca tgatgaagcg gaaataagag
     3961 gccacagaaa aacctatata ggtgccatgc ccgggaggat cattcagaac ataaagcgtg
     4021 caggatcatc aaacccggtg ttcatacttg atgaaataga caaggtggga caggatttca
     4081 ggggagatcc ctcttctgcg ctgctggagg tactggatcc cgaacagaac agttcatttt
     4141 tcgataattt cctcgaaatg gaatatgacc tctccagggt ccttttcatt gccacagcca
     4201 acacacttac aacaataagt cctgctctcc gcgacaggat ggagactata gaaataacag
     4261 gctacctggt tgaggagaag ctggagatag caagccgcca cctgcttccc aggcagctgg
     4321 agaaccacgg ggtgaaggca gaccagcttg aaataggcaa agatgtactg gaaaagctga
     4381 ttgacagcta tacccgtgaa tcaggtgtga gggaacttga caagaggctg gcaaaaattg
     4441 taagggttac cgctaaaaat attgcttcgg aaacaaaata taaccatgat ctgactgaga
     4501 agtctctgga aaagatactc ggaccggcga aatacaacag ggatctttat tccgggaatg
     4561 atcaggcggg agtggttaca ggccttgcat ggacgccggc cggaggcgaa atcctctttg
     4621 tcgaaacgag cataagcaag ggcagcggaa agctcaccct taccggaaat ctcggagaag
     4681 ttatgaagga atcagcaatc cttgcccttg agtatctgaa agcaaatgcc ggacttcttg
     4741 aactgcctga taatttctcg gaaaaatgga atgtgcacct tcatgtgcct gaaggggcaa
     4801 tacccaagga cgggccgtct gccggaatag ccatggccac ttccatagcc tcggccttta
     4861 cccagaggaa ggtgaggaaa tatcttgcca tgaccggtga gataaccctg cgcgggaagg
     4921 tactgccggt cgggggtatc aaggaaaaaa tacttgctgc caaaagagcg aatatccggg
     4981 aaatagttct gtcggaagaa aacaggaaag acgttggcgc cattaaggat atatatataa
     5041 agggtttgaa attcaattat gtcgatagca taatggatgt gcttgatctg gccctgttaa
     5101 ggcagaaagt aagcaatgcc agaaaaatag tgtttgaatg aagaagatag ccgtatttcc
     5161 cggatccttt gatcccttta cccttggtca tgagggtatt gtactgaggg gactgtcgct
     5221 tttcgatgaa ataatcatat cggtgggagc caatgccctg aaaaagaatt attattccct
     5281 tgaaacaaga aaagccatga tttcggaggt cttccggaat gaaccgcgta ttaaggtgga
     5341 tcattatgag ggactgacgg ttgactattg taaaaggcat ggcgccaggt atatcctcag
     5401 aggactgagg actgcagccg atttcgagtt tgaaagggca atagggcagg ttaacaagac
     5461 actcagtccg gaaatagaat cggtattcct gctcactgtt ccggaacaca cgatgattaa
     5521 ttccagcata ataagggata ttatccgcag cggaggtgat gcatccggct ttgtcccgaa
     5581 agcaataaaa ctggaggaat acagggggga ggactagaac ttcagcactg atgtatttat
     5641 catatccatc agtgaaagcc atgcagtgct ctttataatg ctcagttcat cgggcaggag
     5701 ccattccacc ctggtgattc cttcttcagc ctggggttcg gtgaccattt caccatcata
     5761 cttcatcagg aaccatgatg tttttttcag gtaggaaata cccttcaggg tataggtgtg
     5821 atatgtcgga gtcagggatt ttattattga gtgtcccttt attccggctt cctcgcttac
     5881 ttcacggact gcacaggttt ccggcttttc gcccggttca atatgtccct tgggcagatc
     5941 cagccggccc ttcttttcaa taaacagata cctgccggat gaatgtttta ccagtccgcc
     6001 tgccgctcca acctcactga aatagatcct gaaaattttc cagatatgcc ttatgtcggg
     6061 accgtagata tttatagact gcatctctgt atcagtctga aaatcattga taatcctgta
     6121 aagttcatcc gtgtcattga atttaacgaa taagccgtat ttttgtagcc ggtcaggttc
     6181 gggacttatc agaatgaatc tgttttcgaa atgaacttta tacatatctc tatggatact
     6241 tattcaaaaa agatcgcgga atatctttta caaattaaag caataaaatt gcaaccgtca
     6301 aatcccttaa catgggtttc cggctggaag tcccctgttt attgtgataa cagaaaaaca
     6361 ctgtcatttc cgaaggtgag aaaatatatc cgggatgcct ttgtaacggt tataaacgag
     6421 cattatcccg atgcggaact gatagcaggc gtggctaccg gggccatctc acacggtgca
     6481 ctggttgccg acaggctcga acttcccttt gtttatgtta gatcaggtgc aaagacccac
     6541 ggactgggaa accagataga aggcttttat gagccgggtc agaaaacagt ggttatagag
     6601 gatctgattt ccactggcgg cagcagcctt aatgccgtca gaacactcag ggatgccggc
     6661 tgtgaggtac tgggactggc tgcaattttc acatatgaat tcaggaaatc ggaagatgca
     6721 tttattgccg aaaaatgcag gcttgacacc ctgaccaact atacggtcct gattcagacg
     6781 gctctggaaa caggacacat taccgaagat atgatggata ccctgaggga ctggaggctt
     6841 gatccggaaa actggggaag aaactgatta cagccatggc cagcccttca cgttttgaaa
     6901 gccggattgc aaaaatcagt tattcaccgg aaacagttta taattttgct tccgatatga
     6961 ggaatttccg gcgtttcgtt ccttccggca ctttcagcaa cccggatttc aggcaggatt
     7021 cctgcagttt catagtcaat cctgtgggaa cagtaaaggt ttttataaag gacagggttt
     7081 tgtcaaacaa gatagtgtac tcgggaaatg cattactgca gaatgatttt gacatgatcc
     7141 ttgatataag gaattcagcc ggcggtcatt cggaaataaa actctctgtt gaagcggaac
     7201 tgaatccttt cctgctgatg cttgcgaaag agcccctgga taaatttctt gaaaccatta
     7261 tggatgaaat ggagaagttc gaaggatgga atgatgtcag tgcagatact gaatctccct
     7321 gaaggagaat tcacaatatt catttccctc cttttcgacc gtgagaattc ccgagggtga
     7381 aaccccgcgt attgctcccc tgaaaaccac cccgtcagag ctgtaattgt gccactcccc
     7441 ggccctgtaa agatttgaaa aatattcctc ccttattgca tccctgtctc cgtaaagcaa
     7501 ctgcttatat ctgagatcca gaaaatgaag aacctgacgg aataatgaac cggtatcata
     7561 ttcctttccg gtgattattc ccagtgatac ggcacctgat atgcccggac tgaatttttt
     7621 ctggttgata ttgattcctg tgcctacaac ggagtattca atcatctctc ccagaattga
     7681 attttctatc agaatgccgg ctatcttttt cccgccggca taaatgtcgt tgggccattt
     7741 gatatccggc cggggaaggt gattctccag gaaatcgcat atgcccagtg aaattgccat
     7801 tgacagaagg aattgttcat ctgcctgtaa tgatgaagga taaaggataa tgctgaataa
     7861 taaattcatg tcttcagcgc tttcccagct gttccccttc tgtccctttc cggctgtctg
     7921 gaatcctgcc cttatcacag tgccctcctc aggcttctgt tcagtgagca tctgtgccgc
     7981 ggtgacattt gtggatgcaa gtgaatcaaa atacagtatg tcggatccga tgatcatgat
     8041 tatcaaatca ggccggcttc tgccggtgaa attcctactc cgcagacttt taccggaatt
     8101 tgcaatgttg gtgcaaaaat ggcctgctgt gacaaaaata ctgataaaag tttgtatttt
     8161 tgcaacttga aatcagacgg
//
LOCUS       DAOSPX010000131         8140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8347_length_8140_cov_3.866419, whole genome shotgun sequence.
ACCESSION   DAOSPX010000131 DAOSPX010000000
VERSION     DAOSPX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8347_length_8140_cov_3.866419"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLZ75_07680"
     CDS             complement(<1..313)
                     /locus_tag="PLZ75_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOS72312.1"
                     /translation="MAKSVKVVSKEKAVILFSGDSGDGMQLTGTLFSDSAAHYGNDLI
                     TFPDYPAEIRAPQGTVGGVSGFQIHLGNSVIDSPGDSADVLVAMNPAALRANASRVKA
                     GA"
     gene            complement(431..1231)
                     /gene="murI"
                     /locus_tag="PLZ75_07685"
     CDS             complement(431..1231)
                     /gene="murI"
                     /locus_tag="PLZ75_07685"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOS72313.1"
                     /translation="MKNQPIGIFDSGVGGLTVAQSVKQVLPGESLVYFGDTAHLPYGD
                     KSAGAIQYYSRKITGFLLDHGAKVILIACNSASASAFDVLKEEFRDRTALIDVIDPVV
                     DYLGTRNYSRVGIIGTKRTIESGSYENKIKVKSPGTEVVSLATPLLVPMIEEGFVFDD
                     ISNAIIRSYLSHASFSDIQALILACTHYPIIKNQISKIFDFGIEIIDSGKPVSAILKE
                     YLEANHLLNDSGKSDDRFFVSDYTPYFEKVARMFFDGDINLKKADIWK"
     gene            complement(1297..1809)
                     /locus_tag="PLZ75_07690"
     CDS             complement(1297..1809)
                     /locus_tag="PLZ75_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOS72314.1"
                     /translation="MKRYIGIAALVVLMISAGQAQNFKFGHINSDELIRTLPEFDTAN
                     AQLEKLRQELINNLEIMSVELNNKSDAYNKESKNLTELVRQTKEQELIDLNRRIQEFQ
                     VNAQEQLQNKQVELFQPIYARVEKVISDVGKENGFLYIFDVAKGSLLYFDESKSTNVL
                     ALVKTKLGVK"
     gene            complement(1854..2369)
                     /locus_tag="PLZ75_07695"
     CDS             complement(1854..2369)
                     /locus_tag="PLZ75_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOS72315.1"
                     /translation="MKKISVIATILILASATAVAQKYAFVDTEYIRKNIPAFTTAQEQ
                     LDNLSRRWEKEIADGYSVVEQMYKSYQNEAPLLSNDMKIKREEAIVNKEKEMKELQNR
                     YFGMEGELFKKREELVKPIQDEILKAIRDIAVEGSYAVIFDSASGGNILFANPKFDIS
                     DQVLEKLGYKN"
     gene            complement(2392..4905)
                     /gene="bamA"
                     /locus_tag="PLZ75_07700"
     CDS             complement(2392..4905)
                     /gene="bamA"
                     /locus_tag="PLZ75_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOS72316.1"
                     /translation="MIFRELNCILLVLFMALGNIPAYAQEKQEIYDYSREDEYVIGGI
                     SVSGVRYLDVNAIVGISGLRMNQRISIPGDATKNAVQRLWTQGLFSDVRITIESMKSD
                     TVFLDILLQERPRISSIKFNGIRQSESTDITKKINLPVGSQITSYILDNTRKIIRDHF
                     IEKGFFNTTVDFVQKDDPDQPNNIILSINVDKKSRVRIGDITFDGNDSFDDRKLRRQM
                     KDTKKKNLNFFKPSKYIAQKFEDDKEKLIKFYNDNGFRDFEIISDSLYDISDDRIGLQ
                     IKVDEGQQYYLRNVDWVGNSVYSKEELARVFNIEKGAVYNQSLILDRLKGLSGSENAV
                     SNLYLDYGYLFSQLTPVEAKVVGDSIDLEVRIYEGDQAYVNNVIISGNSRTNEHVARR
                     ELYTLPGDLFSKDKILRSIRQLGVLGHFDPEKINPTPMPDPVNGTVDLLYKLEEKAND
                     QFEISGGWGAGMLVGTVGVRFNNVAMRNFFKLREWRPYPSGDGQSLSIRAQSNGRIYQ
                     SYSVSFMEPWLGGKRANSFSVSLYRSLMTNGKKKGEDGRQSMIIDGASVGLGKRLEWP
                     DDYFSIYGELSYQKYNLNNYNYYQFLFENGESNLFSLTARLQRFSVGPNIIYPRSGSS
                     YSISLQVTPPYSYIAGKSMLGVPDEEKYRWIEFHKWVFKTENYFPLTRNDKLVLSSRF
                     AFGYLGHYNRNIGPSPFESFSVGGDGMTGYSFYGREIIALRGYTNQSLTPTAANGAPV
                     GNVYSKITFEVRYPISLNQQATIYGLAFLESGRAWNRLKEYNPFRMNRAAGIGLRANL
                     PMFGLLGIDWGYGFDQVPDPSRFSNANKSQFHFVIGQQF"
     gene            complement(4913..5659)
                     /locus_tag="PLZ75_07705"
     CDS             complement(4913..5659)
                     /locus_tag="PLZ75_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOS72317.1"
                     /translation="MSFREKINMNKLPVHIAIIMDGNGRWAVQHRRERIFGHEQGVES
                     VRQVVEAAGGLGIKYLTLYAFSTENWDRPDDEVSALMRIMVQSLNQEGANLLKNNVKL
                     SAIGDFERLGQDVRMKLSETIDLTSASTGLNLILALSYSSRWEITRAAKKLAADIGKG
                     LLTVETVSEKEMEKRLITYGTPDPDLMIRTSGELRISNFLLWQMAYSEFYFTETLWPD
                     FGKEDFYEAIVEFQNRERRFGKTSEQILEK"
     gene            complement(5638..6357)
                     /locus_tag="PLZ75_07710"
     CDS             complement(5638..6357)
                     /locus_tag="PLZ75_07710"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOS72318.1"
                     /translation="MKRVPWVILFFSFFISSHGQRNSDYGIFGGVSSYIGDINPGRLL
                     YAPGPAAGVLYRYNFHPRHALRGSLLYGMMRGSDRDFSNSFQAARDSSFSAGAGELAV
                     QFEFNFLPFSTQGRHLNFSPYFSAGAAVAYLNTTTAANTHISSIQPVIPLSFGFKVNI
                     WKNMGLEAEYGFRKTFYDNLDGVKNMVSPEDQSLIHNNDWYSFTGIVLTWKIYSRLAG
                     CPAYDDVDKKPKIKKCRSGKR"
     gene            complement(6379..7068)
                     /locus_tag="PLZ75_07715"
     CDS             complement(6379..7068)
                     /locus_tag="PLZ75_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72319.1"
                     /translation="MRRWEAVAGAGPSFFFGDIGGYSRSENFLGLRDITYLQTRYNFN
                     ASLKYRLNRVTNLRLGMSYARLHATDERGSNEGRPFEVSTQIFEPALTAEYYFIKNRA
                     ESSYLFQKRRGRFLWSLLRSLDFYGFAGAGAAYFDANPNEAQRQEMALHGMKDSGVAL
                     VIPAGIATTLVYTPNINFGLELGGRYAFTDWLDAYHKEQYSKANDVYYFLNFTISYKM
                     KTGPRGLPSFR"
     gene            complement(7283..>8140)
                     /locus_tag="PLZ75_07720"
     CDS             complement(7283..>8140)
                     /locus_tag="PLZ75_07720"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOS72320.1"
                     /translation="YSKDSNDRTREAVQKLVSEFLKRDIEVNLYQKAGDTRFSFSREE
                     VTPFTSLAMLPARPDMILSVGGDGTFLETVLKVKDSGIPVAGVNTGRMGFLANIPNEE
                     LGNSVDHLCSGDYQIIERSMLEITSPPGLFNGESSTVLNEITIQKLLLSMIIIHVYVD
                     DVFLNTYWADGLIVSTATGSTAYNLSAGGPILSPGDESIIISPMSPHNLTIRPIVLSG
                     SSRLRMVMEGRTEECLLTCDFRFRKIPISEEIIIVKSKSRLGTVMLKGRDFYSTLRNR
                     LMWGADTRN"
BASE COUNT         2104 a         2031 c         1685 g         2320 t
ORIGIN      
        1 tggctccggc tttcacccgt gaggcatttg cccttagtgc agccggattc attgccacca
       61 gcacatctgc agaatccccg ggcgaatcga ttactgaatt tcccagatga atctgaaagc
      121 ccgaaacacc gccaacagtc ccctggggtg cccgtatttc tgcaggataa tcaggaaagg
      181 taatcaggtc atttccgtaa tgagccgcag aatccgagaa cagggtgccc gtcagctgca
      241 ttccgtcacc ggaatcccct gaaaaaagaa tgactgcttt ttcttttgaa acaactttca
      301 cgctctttgc catggtaagc ctctgtttta ttgcaagacc cggaaccggt aaaataagtc
      361 attaatatat aaaaaattag caataaaatt atcccggaaa cccgaataaa tatcaacagc
      421 agtcagtctg tcatttccag atgtcggcct ttttcaggtt gatatccccg tcaaaaaaca
      481 tcctggccac cttttcaaaa tatggtgtgt aatccgacac gaagaacctg tcatcactct
      541 tccccgaatc atttaagaga tgattagcct cgaggtattc cttcagtatg gcggaaacag
      601 gttttcctga atcaatgatt tcaattccga aatcaaatat cttgcttatc tggtttttta
      661 ttatgggata atgggtgcag gcaagtataa gtgcctgaat atccgaaaag gaagcatgcg
      721 aaagatatga acggataatg gcattgctga tgtcatcgaa aacgaatcct tcctctatca
      781 tcggaaccag caggggtgtt gcgagtgaaa ccacctccgt cccgggggat ttcaccttta
      841 tcttgttctc atagcttccc gattcaatgg tccgctttgt accaatgatt ccaaccctgg
      901 aataattccg ggtgcccagg taatcaacca ccggatctat cacatcaatc aatgccgtcc
      961 tgtccctgaa ttcttccttc aggacatcaa aggcagaggc ggaggcggaa ttgcatgcaa
     1021 tcaggataac cttggccccg tggtcaagga gaaatccggt gattttccgg gaatagtact
     1081 gaattgcacc ggcagacttg tcgccgtagg gaaggtgggc agtatcacca aaataaacca
     1141 gactttcccc tggcagtacc tgcttgaccg actgtgcgac cgtaaggcct cccacacccg
     1201 aatcaaaaat acctataggc tgatttttca cactggctgt atattacaaa aaggccatcc
     1261 ggattttccg gatggccgta tcggtattcc gtaactttat ttcacaccca gtttggtctt
     1321 taccaatgca aggacattgg tgctcttgct ttcatcaaaa tacagcaggg aaccctttgc
     1381 aacatcaaag atgtaaagga atccgttttc cttccccaca tcactgatta ccttctccac
     1441 cctggcgtat atcggctgaa acaattcaac ctgtttgttc tgaagctgct cctgggcatt
     1501 tacctggaat tcctgaatcc tcctgttcag gtcgatcagt tcctgttcct ttgtctgcct
     1561 caccagttcc gtcaggttct tgctttcctt gttgtaggcg tcacttttat tgttaagctc
     1621 aaccgacatt atctcaaggt tattgataag ctcctggcgg agtttctcaa gctgggcatt
     1681 ggcagtatcg aattccggaa gggtccttat cagttcatca ctgtttatat ggccgaactt
     1741 gaaattctgt gcctgaccgg cagaaatcat taaaacaaca agagctgcta ttcctatata
     1801 ccttttcatt atactactgt gtattaaatt ttaacctctg caaaagtaat tatttaattc
     1861 ttataaccta atttttctag aacctgatcg ctgatatcaa atttggggtt cgcaaaaaga
     1921 atattgcctc ccgaagccga atcaaaaatg actgcatagc tcccttcaac ggctatatcc
     1981 ctgatggcct taagtatttc atcctgaatt ggcttcacca gctcttccct ctttttaaac
     2041 aattccccct ccatgccgaa gtatctgttc tgaagctcct tcatttcctt ttccttgtta
     2101 acaattgcct cttccctctt tattttcatg tcatttgaca gaagcggggc ctcattctga
     2161 tatgacttat acatttgctc aaccacggaa taaccgtcgg caatctcctt ctcccagcgc
     2221 cttgaaaggt tgtccagctg ctcctgggcg gttgtgaatg cgggtatgtt cttcctaata
     2281 tattcggtat cgacaaatgc atatttctgg gccacggccg tggctgaagc cagaatcagt
     2341 attgttgcta tgacgctgat ttttttcatg actgtaattt ttttcattca attaaaactg
     2401 ctgacctatg acaaaatgga actgcgattt atttgcattg gagaatcttg aaggatcggg
     2461 cacctgatcg aagccgtagc cccagtcaat tccaagcagc ccgaacatcg gaaggttggc
     2521 tctcagtccg attccggccg ccctgttcat cctgaacggg ttatactctt tcagcctgtt
     2581 ccatgccctt cccgattcca ggaaggccag accgtatatt gtcgcctgct ggttaaggga
     2641 tatgggatat cttacctcaa aagttatttt ggaatagaca tttccgaccg gggccccgtt
     2701 ggctgcagtt ggtgtaagcg actgattggt ataaccccgc agggcaatta tttcgcggcc
     2761 gtagaagctg tatccagtca ttccgtctcc tcccacactg aacgactcaa agggagaagg
     2821 tcctatgttt ctgttgtagt gtccaagata tccgaatgcg aaacgtgaac tgagaacaag
     2881 cttgtcattc cttgtaaggg gaaaatagtt ctccgtcttg aaaacccatt tgtgaaattc
     2941 tatccacctg tatttttcct catcaggaac ccccagcatg gattttccgg cgatatagct
     3001 gtagggcggg gtgacctgaa gtgatatgga gtaggaggaa ccgcttctgg gataaataat
     3061 gttcggaccc accgaaaatc tctgaagcct tgcggtaagg ctgaaaaggt tggattcccc
     3121 gttctcgaac aggaactggt agtaattgta gttgttgaga ttgtatttct gatagctcag
     3181 ttcaccatat atggagaaat agtcatcagg ccattccagt ctttttccca gacctacaga
     3241 tgctccgtca attatcattg actggcgtcc gtcctctccc ttcttcttgc cattggtcat
     3301 cagcgacctg tagagtgaaa cggagaagga gtttgccctt ttcccgccca gccacggctc
     3361 catgaaggaa actgaatagg actggtatat ccggccgttt gactgtgccc tgatgctgag
     3421 cgactgtccg tcacccgagg gataaggccg ccattctctg agcttgaaga aattcctcat
     3481 tgccacgttg ttgaatctca ctcccacggt acccaccagc attccggctc cccatccgcc
     3541 ggatatttca aactgatcat tggctttctc ctcgagcttg tacagcagat ccaccgtccc
     3601 gttgacagga tcaggcatgg gtgtcggatt tattttttcc gggtcgaaat gtccgagaac
     3661 accaagctgg cggattgaac gaagtatctt gtccttgctg aaaagatccc ccggaagcgt
     3721 gtacagctca cgccgggcaa catgttcatt ggtccgggag ttgccggaaa ttatcacatt
     3781 gttgacatag gcctgatcac cctcgtatat tcttacttca agatctatgg aatccccgac
     3841 caccttcgcc tcaacaggcg tcagctgtga aaacagatat ccgtaatcaa ggtacagatt
     3901 gctcacggca ttctcggatc cgctcagtcc cttgagcctg tcaaggatga gcgactggtt
     3961 gtaaacagcc cccttctcaa tgttgaagac ccttgccagt tcttccttgc tgtaaacgct
     4021 gttccccacc cagtcaacat tcctgaggta atactgctgg ccttcgtcca ctttgatctg
     4081 cagccctatc ctgtcgtcgg atatatcgta aagggagtcg gaaatgattt caaaatccct
     4141 gaatccgtta tcattgtaaa atttgatcag cttctccttg tcatcttcaa atttctgggc
     4201 aatgtatttg gatggcttga agaaattcag gttcttcttc tttgtgtcct tcatctgccg
     4261 cctgagcttc ctgtcatcaa agctgtcatt cccgtcaaag gtgatgtccc ctatccttac
     4321 cctcgacttc ttgtcgacat ttattgacag tatgatattg ttcggctgat cgggatcatc
     4381 cttctggaca aaatccacgg ttgtattgaa aaagcccttt tcaataaaat gatccctgat
     4441 aattttcctg gtgttgtcaa gaatatagga tgtgatctgt gaaccgacag ggaggttgat
     4501 tttctttgta atatccgtgg attcggactg tcttatccca ttgaacttga tagatgatat
     4561 cctcgggcgc tcctgcagca gtatatccag aaaaaccgta tccgacttca ttgattcgat
     4621 tgttattctc acatccgaga aaaggccctg tgtccataat ctctgaactg cattttttgt
     4681 agcatcaccg ggaatgctga tccgctgatt cattcttaat cccgaaatgc ccactatggc
     4741 gttgacgtca agatacctga ctcctgaaac ggaaattccg cctattacat attcatcttc
     4801 cctggaataa tcataaatct cctgtttttc ctgcgcatat gccggaatat tgcccaatgc
     4861 cataaacagt accaataaga tacagttaag ctccctgaat atcatccttc tactattttt
     4921 ccaaaatctg ttcactcgtt tttccaaatc ttctttccct gttctggaat tcaactatgg
     4981 cctcataaaa atcttccttg ccaaaatcag gccaaagtgt ttcagtaaaa taaaattctg
     5041 aataggccat ctgccataac aggaaattgc ttatcctaag ttccccgctt gtccttatca
     5101 tcagatcggg atcaggcgtc ccgtatgtga taagccgttt ttccatttcc ttttcactga
     5161 ctgtttccac cgtgagcaat ccttttccga tgtcggcagc cagctttttt gccgcccggg
     5221 ttatttccca tcttgaactg tagctgaggg caagtatcag attgagacct gttgaagccg
     5281 aggtcagatc gatggtctca gacagtttca tcctgacatc ctgtccaagc ctttcaaaat
     5341 caccgatggc ggaaagcttt acattattct tcagaagatt ggcaccttcc tggttaagag
     5401 actgaaccat aattctcatc agggccgata cttcatcatc aggcctgtcc cagttttccg
     5461 tggaaaaggc atagagagtg agatatttta ttccaaggcc ccctgcagcc tcaacaacct
     5521 gccggactga ttcaactccc tgttcgtgtc cgaaaatcct ttccctcctg tgctgtacgg
     5581 cccatcttcc gtttccgtcc atgattatgg caatatggac cggaagcttg ttcatgttta
     5641 tcttttccct gaacgacatt tcttaatctt tggttttttg tcgacatcat cgtatgccgg
     5701 acaaccagcc agtctgctgt aaattttcca cgtcagtaca ataccggtaa atgaatacca
     5761 gtcgttatta tggatcagac tttgatcctc cggagaaacc atatttttta cgccgtctaa
     5821 gttatcataa aaagttttac gaaatccata ttcggcctcc agtcccatat ttttccagat
     5881 attgaccttg aatccaaagg agagcggaat gacgggctga atggacgaaa tatgtgtatt
     5941 ggctgctgtt gtggtattta agtatgccac agctgcgcct gcggaaaaat aaggagaaaa
     6001 attcagatgc ctgccctggg ttgaaaaagg aagaaagtta aattcaaact gaaccgccag
     6061 ttcacctgca cctgcagaaa acgacgaatc tcttgctgcc tggaaactat ttgaaaaatc
     6121 cctgtctgac cccctcatca tgccgtaaag cagactgccc ctcagggcat gccggggatg
     6181 gaaattatac ctgtatagga ctcctgcagc cggaccggga gcataaagaa gtcttccggg
     6241 attgatatca cctatgtagg aagatacacc cccgaatatt ccatagtcag aattacgctg
     6301 gccatgggag gaaataaaaa agctaaaaaa aagaattacc cagggtactc gcttcattaa
     6361 aaaagattcc gcttattatt atctgaatga gggcaacccg cgtggccctg ttttcatttt
     6421 gtatgaaatt gtaaagttca aaaaataata aacgtcattt gccttggaat attgttcttt
     6481 atgataggcg tcaagccagt cggtgaaagc atagcgcccg cccagctcca ggccaaaatt
     6541 gatatttggg gtatatacaa gcgttgttgc aattcctgcc ggaatgacaa gggcaactcc
     6601 gctgtccttc atgccgtgca aggccatttc ctgcctctgg gcctcattgg gatttgcatc
     6661 gaaataagca gcacctgctc ctgcaaatcc atagaaatca agcgatctca gaagggacca
     6721 caaaaacctg cccctcctct tctgaaaaag ataactgctt tcagccctgt tttttataaa
     6781 ataatattca gcggtaaggg ccggttcaaa aatctgcgtg gaaacctcaa agggcctccc
     6841 ctcattcgag cctctctcat cggtggcatg aagccgggca tatgacattc caagcctcag
     6901 gttcgtcaca cggttcaacc ggtatttcag actggcattg aagttgtatc ttgtctgaag
     6961 ataggtgata tcccgcagcc cgagaaaatt ctcactccgg ctgtagcccc ctatgtcccc
     7021 gaagaaaaaa gacggtccgg ctccggcaac tgcctcccat ctcctcattt tccataactg
     7081 ggcactggaa aagggtacag caagccaaaa gatcagtaga accaaaatgt atcgcttcat
     7141 aatgattgac aatagttgca aatacaaata tagcaatcta aatttcagga agtataaaaa
     7201 tcagttataa tattaacata tacacatgaa tatatcaacc gtgcaatgtt gaaaagcata
     7261 aatgacagcc gatggtaaaa tttcagtttc ttgtgtcagc accccacatc agcctgttcc
     7321 tcagggtact gtaaaaatct cttcccttca gcataaccgt tccgagcctg gatttgctct
     7381 taactatgat tatttcctca cttatcggta tctttctgaa tctgaaatca caggtaagaa
     7441 ggcattcctc cgttcttcct tccataacca tcctcagcct gctgctcccg gaaaggacta
     7501 taggcctgat tgtaagatta tgcggcgaca tcggtgaaat aatgatgctc tcatcaccgg
     7561 gcgacagtat tggtcctccc gcactcagat tatatgccgt ggagccggta gccgtggata
     7621 caatcagacc atctgcccag taggtattca ggaatacatc atccacatat acatggatta
     7681 tgatcatact gagcagcagt ttctgaatgg tgatctcatt cagtacagtg gatgattccc
     7741 cgttgaaaag gcccggcgga ctggttattt caagcattga cctttcaatt atctgatagt
     7801 cgccggaaca gagatggtca accgaattac ccagttcctc attcggtatg ttcgcgagaa
     7861 agcccattct gccggtattg actcctgcaa cagggatacc ggaatccttg accttaagga
     7921 ctgtttcaag aaaggttccg tcgccgccga cactgaggat catgtcaggc cttgccggaa
     7981 gcatggcaag actggtaaag ggagtaacct cttccctgct gaaactgaat cttgtatcac
     8041 cggctttctg atagagattt acctctatgt cccttttaag aaattcactt acaagtttct
     8101 gaacagcctc ccgtgtccgg tcattgctgt ccttgctgta
//
LOCUS       DAOSPX010000132         8101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8400_length_8101_cov_4.262615, whole genome shotgun sequence.
ACCESSION   DAOSPX010000132 DAOSPX010000000
VERSION     DAOSPX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8400_length_8101_cov_4.262615"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PLZ75_07725"
     CDS             complement(<1..665)
                     /locus_tag="PLZ75_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS72321.1"
                     /translation="MAKTKTVFICQNCGAESPKWIGRCPSCREWNSYQEEIVSPVSEG
                     KPGFFRELEKRNPRLLDRIEADEGKRHKTGIPELDRVLGGGMVSGSLLLLGGEPGVGK
                     STLALQLALSLKGINVLYVSGEESEEQISLRAQRLKLSNPSCYVLSETRLENILAHCE
                     KLNAGLVIADSIQTMTTDSLESSAGSVSQVRECASRFLMYSKLTGTPVVLIGHITKDG
                     TLAG"
     gene            complement(790..1833)
                     /locus_tag="PLZ75_07730"
     CDS             complement(790..1833)
                     /locus_tag="PLZ75_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOS72322.1"
                     /translation="MKKEVKQVLQFIIFLALGLLLLWVAFRNVRFRELAEGLKSANYL
                     FVLLSVAFSIFAYLSRARRWNLLIHPLGYKPSFMNTFNALMTGYFANLALPRIGEITR
                     CVALGKKEDIPADKLIGTVVVERAVDMMALLIIMTVMIFTSGDRIRQFLKETIFIPVK
                     EQILIPAGQTWLIWSLVLLILAVLLWLVVRYRKRLGKIPLVSEIFKLSKGIITGLKSI
                     TRLEKKWEFLFHTVFMWICYIFMTWVVVFAIDNTSHLGFRDAIFLLVIGGFAMVVPVP
                     AGMGAFHYIISRGLAFVQGVNLEDGALYAILTHESQVIMVIIVGIVSSYMIFRKDKGK
                     EAAEKSADSASAK"
     gene            2076..2882
                     /locus_tag="PLZ75_07735"
     CDS             2076..2882
                     /locus_tag="PLZ75_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOS72323.1"
                     /translation="MESRKVLFVSQEITPYIAENRLSRIGRYLPQGIQERGKEIRTFM
                     PRYGCINERRNQLHEVIRLSGMNLIIDDTNHPLIIKVASIQSARMQVYFIDNDDYFQR
                     KFLVTDASGREFEDNDERALFFARGVIETVKKLRWAPDIVHCHGWMSALVPVYLKSIY
                     SDDPLFNNQKIIYSVYNNDFKTPFRSSFADKLRYDGIDGESLKLVKNADFINLSKLAI
                     KYSDAIIIGSENINEELKTHLETVKKPVLSFRDESEYIDAYNDFYDEMLS"
     gene            2845..4218
                     /locus_tag="PLZ75_07740"
     CDS             2845..4218
                     /locus_tag="PLZ75_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOS72324.1"
                     /translation="MPTMIFTMKCCHSCLSGRNLFIRNILKILGPVLLAGMLLSVASC
                     EEDPSRIGRKLLPGDDFVSILSTDTVRIRSYTTYNPAVKSGNPAVSYLGQIHDPYFGT
                     IDARFVSQIRLGAAWTPGEYVIDSIRLYLEFLSVTGATDKPQFLSFMEIEKQIYNDSV
                     YYSNQAVGLTGFGVTGIPLPRLKPDSANYIKLDIPTEFGEYITRDTTMLFHHSTKPDF
                     RSYFRGLLFEITSPEEPVFVSLSVDPPGKYETYQNYFIMFMHDAEGHKKQYYFILDAF
                     SRNAAFNLFSHDHTAADPDKRIMHINDGYADTLSYVQIMNGLNTRLMIPGLSDIKNDP
                     EMKNISINKARLIIPVHYDRKIYKPSTIPSRIFLNYITKSGRKRTVPDYSPMNPGFYD
                     GTPDTTNNVYNINISTWLQNYLKDTSDSLTTDFELYLLPTSSNNVILKANNSRTPVRF
                     EITYTRF"
     gene            4289..6289
                     /locus_tag="PLZ75_07745"
     CDS             4289..6289
                     /locus_tag="PLZ75_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOS72325.1"
                     /translation="MELPAEYRNLSDSEIAIMKAAGCRCDEWNRVKVADGFDPYHCIN
                     VTFSGDIRLGRFTRIFTDESGVTTRSGISNAHLHNCRVGSDVLIANIGDYIANYNIEN
                     DVVIKNCGKIHTQKRSSFGNGTEVAVLNETGGRAVKIWDRLSAHEAYVCALYRHRVKA
                     VARIEEMVREYSDSVASGTGTIGRNTKILNCRDIRNVRFGPSTRVDGAIRLNEGSVNS
                     CPEDPVFIGPGVIMDHFIVCSGSIVTDSTLVDKCFIGQGCVLGKHYSAENSLFFANCA
                     GFHGEACSIFAGPYTVTHHKSTLLIAGIFSFMNAGSGSNQSNHMYKLGPIHQGIMERG
                     SKTTSDSYVLWPARIGPFTLVMGRHYNNVDTSSLPFSYLIERDDETILVPGVNLRSVG
                     TIRDAQKWPRRDRRKDPQLIDQVNFNLLSPYTIRKMFEGRDLLRKLKSVAGAVSSSYA
                     YNNMLIKGSALETGIEMYQKGINKFLGNSLIKRLENMEFRSVHDLRERLKPEGCPGDG
                     EWVDLAGLIAPQKVVDRLLDDIESGKTGTLGAIAEEFRKMHENYYNWEWTWACERLEQ
                     ELGRPVSSFTAADVVNMVEKWKESVIGLDRMLYEDARKEFNLSSRTGFGIDGGEEIKK
                     LDFEQVRGDFESNGVVYAIQEHIIQKTALGDELIRRMEAIDR"
     gene            complement(6294..7355)
                     /gene="rfbB"
                     /locus_tag="PLZ75_07750"
     CDS             complement(6294..7355)
                     /gene="rfbB"
                     /locus_tag="PLZ75_07750"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOS72326.1"
                     /translation="MKYRKKILITGGAGFIGSHVVRRFVTNHPDYLIVNADKLTYAGN
                     LENLKDIESRDNYLFVKTDIVSKQSVTELFDRHGFNGVIHLAAESHVDRSISSPDEFV
                     FTNIVGTVNLLNAALHSWKDGFEGRLFYHISTDEVYGSLGSEGTFTEETPYDPKSPYS
                     ASKASSDHMVRAYNHTFGIPAIISNCSNNYGPNQFPEKLIPLVINNIKNNKPIPVYGR
                     GENVRDWLFVEDHALAIDIIYHRGRAGETYNIGGNNEWKNIDLIRLLCRIMDKKLGRP
                     GGSSEKLITFVKDRPGHDLRYSIDSSKLQKELGWQPVPDFEALLENTVDWYLTNSAWL
                     ENVLSGDYEKYYDSMYSGR"
     gene            complement(7360..>8101)
                     /gene="galE"
                     /locus_tag="PLZ75_07755"
     CDS             complement(7360..>8101)
                     /gene="galE"
                     /locus_tag="PLZ75_07755"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOS72327.1"
                     /translation="LEYYRNNLVSLMNLLDSMRSFSIPHMVFSSSCTVYGQPAILPVT
                     EDAPVQPAVSPYGNTKQIGEEIIRDAVAAYEQINAISLRYFNPIGAHASALIGELPRG
                     VPENLVPFITQTAYGLRDELKVFGVDYDTPDGSCIRDYLHVVDLAKAHVIAVERLISG
                     KNKNPYEVFNLGTGKGVSVLEAISAFERATGVSLRYSITGRRPGDIEKIWADPSLANR
                     ELGWKTLSTLEECMRTAWAWENEIRKKK"
BASE COUNT         2107 a         1921 c         1917 g         2156 t
ORIGIN      
        1 ccggccagtg tcccgtcctt ggtaatatga cctatcagca caaccggtgt tcccgtaagt
       61 tttgaataca tcaggaagcg ggaggcacat tcgcggacct gcgacacgga accggcagat
      121 gactcgaggg aatccgttgt cattgtctga attgaatcgg caatgacgag tccggcattg
      181 agtttttcac aatgcgcgag aatattttcc agccgggttt cactgaggac atagcatgag
      241 ggattggata acttcagccg ctgtgccctc aggcttatct gttcctcgct ttcctcgccc
      301 gacacgtaca gcacattgat ccctttcagg gacagtgcaa gctgaagcgc aagagttgat
      361 ttccccaccc cgggttctcc tcccagaagt aaaagggatc cgctgaccat gccgccgccc
      421 agaaccctgt caagttcggg aatccccgtc ttatggcgtt tgccttcatc agcctctatc
      481 ctgtcgagca gcctgggatt cctcttttcc agctcccgga agaatccggg ctttccctct
      541 gaaacgggac tgactatttc ctcctggtag ctgttccatt ccctgcatga agggcatctg
      601 cctatccatt tgggtgattc ggcgccacaa ttctggcaga taaatactgt tttggtcttt
      661 gccatatctg attcgctagg attattatct gaggggattt ttcttccttc gttttccgga
      721 cctgcagagt tctcctcgtt ttttccggtc cgcagctttc cacttctttc tcccggtcag
      781 tcctcttaat tatttagcgg aagcactgtc tgctgacttt tcggctgctt cctttccttt
      841 atcctttctg aaaatcatat aggaagaaac gattcccaca ataattacca taataacctg
      901 ggattcatgt gtcagtattg cgtaaagtgc gccgtcctcc aggttgactc cctgaacaaa
      961 agcgagtccg cgggatatta tatagtggaa ggcacccatc ccggcaggta ccgggacgac
     1021 catggcaaat ccccctatta ccaggaggaa tattgcatca cggaatccaa ggtgggatgt
     1081 attgtcaatt gcgaaaacaa ccacccatgt catgaatatg tagcagatcc acatgaatac
     1141 cgtatggaac aggaattccc atttcttttc aagcctggta atagatttca gtcctgtaat
     1201 tattcccttt gataatttga agatttccga aaccaggggt atttttccga gccttttgcg
     1261 gtatctgacg acgagccaga ggagtactgc cagaatgagc agcacaaggc tccagatcag
     1321 ccatgtctga ccggcaggga tcagtatttg ttccttaacc gggatgaata tggtttcttt
     1381 cagaaattgc ctgatcctgt cgccgctggt gaagatcatt accgtcatga tgatcagcag
     1441 tgccatcata tcaaccgccc tttcgacaac aactgttcca ataagcttgt cggcaggaat
     1501 atcctccttt ttccccagtg ccacgcaacg ggttatttcc ccgattctcg gcagtgccag
     1561 attggcgaaa taaccggtca tcagtgcatt gaaggtattc atgaaagaag gtttgtaacc
     1621 caggggatga atgagaagat tccatcttct tgcacggctg aggtaggcaa aaatgctaaa
     1681 ggcaacagat aacaggacga aaaggtaatt tgctgattta agtccctctg ccagttccct
     1741 gaatcttaca tttcggaaag caacccacag aagcaataat cctaatgcga gaaagattat
     1801 gaactgcaga acctgtttta cttctttttt caagtccggt ccgctttatt taaaatatgc
     1861 cgcaaagtta attatccttt tctatttcct gaagggggcc ggctttcaat tttgccactt
     1921 tgaccagcgg ttcctgtccg gtaaaaatac aggcaaaaat catcaaaaaa ctgagagaca
     1981 aggctgataa ttcatgtaaa caccttatta tcattatctg aaaatttatt attatctttg
     2041 caacttgtaa cccaacggaa ttttgaggca ggagaatgga aagcaggaag gttttattcg
     2101 tttcacagga gataactcca tacatagcag aaaacaggtt atccaggatc ggaaggtatc
     2161 ttccccaggg aattcaggaa agggggaagg agataagaac cttcatgcca aggtatggct
     2221 gtatcaatga gcgcaggaat cagcttcatg aggtcatcag gctgtccgga atgaatctga
     2281 tcattgatga caccaatcac cccctgatta taaaggtagc atcaatacag agtgcaagga
     2341 tgcaggttta tttcattgac aatgatgatt atttccagag gaaatttctg gtaaccgatg
     2401 catcgggacg tgaatttgaa gataatgatg aaagggcact cttttttgcc cggggagtga
     2461 ttgaaacagt caagaaactt cgctgggcac ccgatatcgt gcattgtcac ggatggatgt
     2521 cggccctggt accggtttac ctgaagagta tctattctga cgatcccctg ttcaataacc
     2581 agaaaattat ctattccgtt tacaataatg acttcaaaac accgttcaga tcatcattcg
     2641 cagataaatt gcgttatgac ggaattgatg gcgaaagcct gaagctggta aaaaatgccg
     2701 actttatcaa cctttcaaaa ctggccataa aatattccga cgcaataatc atagggagtg
     2761 agaatataaa cgaggaattg aagacacatc tggaaactgt taaaaaaccg gttctttcct
     2821 tcagggatga gtcagagtat atagatgcct acaatgattt ttacgatgaa atgctgtcat
     2881 agctgccttt ccggcagaaa cctgtttatc cggaatattc ttaagatcct tggtcccgta
     2941 ctgcttgcgg gcatgcttct ttctgtcgcc tcctgtgagg aagatccctc caggataggc
     3001 agaaagcttc tgccgggaga cgattttgtc agtatactgt caaccgacac cgtcaggatc
     3061 aggtcctata ctacctacaa tcccgcggtt aagtcaggca atccggcagt ctcctatctc
     3121 ggacagatcc atgatcccta tttcggcacg atagatgcgc gctttgtttc tcagatcagg
     3181 ctgggtgccg cctggacacc gggtgaatat gtcattgatt caataaggct ttatcttgaa
     3241 tttctctcag tgaccggagc aacagacaag ccccagttcc tttccttcat ggaaatagag
     3301 aaacagatat ataatgattc agtctattac tccaatcagg cagtcgggct taccggcttt
     3361 ggcgtgacgg gcattcccct gcccaggctc aaacctgatt cggcaaacta catcaaactg
     3421 gatattccca cggaattcgg cgaatatatc acgcgtgaca ccactatgct tttccaccat
     3481 tccacaaaac ccgatttcag atcctatttc aggggtctcc tgtttgaaat cacttcgccc
     3541 gaagaaccgg tatttgtgtc actgagtgtt gatccgccgg gaaaatacga aacatatcag
     3601 aactatttca tcatgttcat gcatgatgcc gaaggacata agaagcagta ctatttcatc
     3661 ctggatgcct tcagccgcaa tgcggccttc aacctcttca gtcatgacca taccgctgct
     3721 gatcctgaca agaggattat gcacatcaat gacggatatg ccgataccct ttcctatgta
     3781 cagatcatga acggacttaa taccaggctc atgattccgg gcctgagtga cattaaaaat
     3841 gatccggaaa tgaaaaatat ttcgataaac aaggccaggc tcattattcc cgttcattac
     3901 gacaggaaaa tctataaacc ttcaaccata ccttccagga tatttctgaa ctatattact
     3961 aaaagtggca ggaaacggac ggttcctgat tattccccga tgaatccggg attttatgac
     4021 ggcaccccgg acacaacgaa taatgtttac aatatcaaca tctcaacctg gctgcagaat
     4081 tatctgaagg atacatctga cagtctcacc accgatttcg aactctacct tctgccgaca
     4141 tcatcaaaca atgttattct gaaggccaac aacagccgta cacctgtcag atttgaaata
     4201 acctatacaa gattctagaa aatcggctga atattattat attcgtatcc gaaagtcagt
     4261 gagttaagta agaatacaag tatcccatat ggaacttccg gctgaatacc gcaatctcag
     4321 cgattcggag attgccatca tgaaagctgc gggctgccgc tgtgatgaat ggaaccgtgt
     4381 gaaggtggca gacgggtttg atccgtatca ctgtatcaac gtcacgtttt cgggagatat
     4441 cagactggga agattcacca ggatatttac cgatgaatcc ggggttacaa ccaggagcgg
     4501 aatctcaaat gcacaccttc acaactgcag ggttggctcg gatgtcctga tagccaatat
     4561 cggagactat attgccaact acaacattga aaatgacgtt gtcattaaaa actgcgggaa
     4621 gatacacact cagaaaagat cctccttcgg caacgggact gaggtagccg tcctcaatga
     4681 aacaggcggc cgggcagtta aaatctggga ccgcctgtca gctcatgagg cttatgtatg
     4741 tgctctctac cgccaccggg tgaaggctgt cgccaggatt gaggagatgg tcagggaata
     4801 ctcggattcg gttgcttccg gcaccgggac aatcggccgg aatacgaaga tccttaactg
     4861 ccgtgacatc cggaatgtaa ggtttggtcc cagcacccgc gttgacgggg ccatcaggct
     4921 caatgagggt tcggtgaaca gctgtccgga agatccggtt tttatcggtc ccggggtgat
     4981 catggatcat ttcatagtgt gttccggatc aatagtaact gattcaaccc ttgttgacaa
     5041 atgcttcatt ggccagggct gtgtgctcgg aaagcattac agcgctgaaa attccctttt
     5101 ctttgccaac tgtgcgggtt ttcacgggga agcctgttca atatttgcag gaccatatac
     5161 ggttacccat cataagtcaa ccctccttat tgcaggcatc ttttcattca tgaatgccgg
     5221 cagcgggtcg aaccagagca atcacatgta caagctcggt ccgatacatc agggcataat
     5281 ggaaagaggt tcaaaaacga ccagtgattc ctatgtcctg tggcctgcca ggattggtcc
     5341 attcacactg gtgatgggcc ggcattacaa taatgtggac acctcatcac tgcccttttc
     5401 ctatctgatt gaaagggatg atgagactat tctggtaccg ggggtgaacc tcagaagcgt
     5461 cggaaccata agggatgccc agaaatggcc ccggcgcgac agaagaaagg atccgcagct
     5521 gattgaccag gtcaatttca acctcctgag tccctatacc ataaggaaaa tgttcgaagg
     5581 cagggatctt ctccggaagc tgaaatcggt tgcaggagcc gtatcctctt cctatgccta
     5641 caataatatg ctgatcaagg gatctgccct tgaaacgggt attgaaatgt atcagaaggg
     5701 gataaacaag tttctcggca attcactgat caaaaggctt gaaaatatgg aattccgctc
     5761 cgtccacgat ctccgggaaa ggctgaaacc ggaaggctgc ccgggtgacg gggaatgggt
     5821 tgatcttgcc ggccttattg caccgcagaa ggttgttgac aggctgcttg acgatattga
     5881 atcgggaaag accgggactc tcggagccat agcggaggag ttcaggaaga tgcatgagaa
     5941 ttattacaac tgggaatgga catgggcatg tgaaaggctg gaacaggagc tgggcagacc
     6001 cgtaagcagc tttactgccg ccgatgtggt gaatatggtg gaaaaatgga aggaaagcgt
     6061 gataggtctt gacaggatgc tttatgagga tgccaggaag gagttcaatc tatcttcgcg
     6121 tacaggcttc ggaatagacg gaggggaaga gatcaagaaa ctcgacttcg aacaggtgag
     6181 gggcgacttc gaatcaaacg gcgttgttta cgccattcag gaacatatca tccagaaaac
     6241 agccctggga gacgagctca tccggaggat ggaagcaata gaccggtgat cctctatctc
     6301 ccggaataca ttgaatcgta atatttttcg taatcacccg acaatacatt ttcaagccag
     6361 gctgagttgg tgagatacca gtccaccgta ttttccagca gtgcctcaaa atccggaacg
     6421 ggctgccagc ccagttcctt ctgaagcttt gatgaatcga ttgaatatct caggtcatgc
     6481 ccgggcctgt cctttacaaa ggttatgagc ttttcggagc ttcctccggg ccgtcccagc
     6541 tttttgtcca taatcctgca caggagcctg atgaggtcaa tattcttcca ttcattgttg
     6601 ccgccgatgt tataggtttc tcccgccctt cccctgtgat agataatgtc tattgccagg
     6661 gcatggtcct ccacaaaaag ccagtcgcgg acattttccc ccctgccgta gacaggtatc
     6721 ggcttattgt tttttatatt atttattacc agggggatca gtttttcagg aaactggttt
     6781 ggtccgtaat tgtttgaaca gtttgaaatt atcgcgggta ttccgaaggt atggttatag
     6841 gctctgacca tgtggtcgga actggctttt gaggcggaat acggactttt cgggtcatag
     6901 ggggtttctt cggtgaaggt gccttcactg cccagtgagc cgtacacctc gtctgttgag
     6961 atatggtaga agagccggcc ctcaaacccg tccttccagc tgtgaagggc tgcattcagg
     7021 agattcaccg ttccgacaat gttggtgaac acaaattcgt cgggactgct gattgatctg
     7081 tcgacgtgcg attccgcggc aaggtgaatg acaccgttaa acccgtgcct gtcaaagagt
     7141 tctgttaccg actgtttgct tacaatgtcg gtttttacaa aaagatagtt atcccttgat
     7201 tcgatgtcct tcaggttttc aagattcccg gcataggtga gcttgtcggc attcacaata
     7261 aggtaatcgg gatgattcgt gacaaatctc cttacgacat gggatccgat gaatccggct
     7321 cctccggtaa tgaggatttt ttttctgtat ttcatatgct tattttttct ttcttatttc
     7381 attttcccat gcccatgctg ttctcatgca ttcctccagc gtgctgaggg ttttccatcc
     7441 cagttccctg tttgccagtg agggatcggc ccatattttc tcaatatccc cgggtcttct
     7501 tcccgtgatg ctgtagcgca ggctgacacc tgttgccctt tcaaaggcag atattgcttc
     7561 cagaacggat actccctttc cggtgcccag gttgaagacc tcatagggat ttttattttt
     7621 tccggagatc agtctttcca ccgcaattac atgagctttc gccaggtcaa caacatgaag
     7681 gtaatccctt atacatgaac cgtcaggagt gtcataatca accccgaaca ctttcagttc
     7741 atccctcagt ccgtatgcgg tctgggttat aaacggaacc aggttttcgg ggactccccg
     7801 gggcagttcg ccgatcagcg cggaggcatg ggctccgata ggattgaagt atctcagtga
     7861 gatggcattt atctgttcat aggcggcaac ggcatccctg attatttcct caccgatctg
     7921 ctttgtgttt ccgtagggtg aaacagcggg ttgcaccggc gcgtcttccg ttacgggcag
     7981 aatggcgggc tggccgtaaa cggtgcatga tgacgagaag accatgtgag ggatgctgaa
     8041 cgatctcatt gaatccagca gattcatcag ggaaacaagg ttgttccggt agtattcaag
     8101 g
//
LOCUS       DAOSPX010000133         8074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8423_length_8074_cov_4.211622, whole genome shotgun sequence.
ACCESSION   DAOSPX010000133 DAOSPX010000000
VERSION     DAOSPX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8423_length_8074_cov_4.211622"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLZ75_07760"
     CDS             <1..485
                     /locus_tag="PLZ75_07760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS72328.1"
                     /translation="RYTSGVSLIVAGSGDVIGRLKNRVLDLELADRILFLDMMPWEEL
                     MKYTRSADAGLSLDKDTNMNYRFSLPNKLFDYISAGIPVIAGNLPEVAGIIKKYDCGI
                     IIPEITPEAIAEAVETLKNDRELLNKLRRNVVYASEDLSWEKESEKVKKFYERVFKEK
                     "
     gene            490..699
                     /locus_tag="PLZ75_07765"
     CDS             490..699
                     /locus_tag="PLZ75_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOS72329.1"
                     /translation="MVIFTISGLAGGFLYWKFVGCLSGTCPIKSVWYWSTLYGGLIGY
                     LAGSILLDVIRKFSKRKGINDSSEN"
     gene            977..1651
                     /locus_tag="PLZ75_07770"
     CDS             977..1651
                     /locus_tag="PLZ75_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOS72330.1"
                     /translation="MTTGKKLYYSAFALLGATLLLFSSCKKENVPDPSTVDADGNVYP
                     TVVIGGQEWFAENLRTTKYNDGTSIPNVTKSWEIQSTDAYCWYNNEPDNGNKYGALYN
                     WLAVKTGKLCPTGWHVPSVEEWQALMDYAAPDNAVKLKSTSGWYNNGNGTDDYGFSAL
                     PGGYRDVFFKDLGLYGHWWTSYGFVHEQVWELAESREMYWGYKTVYRALVYSSIGYSV
                     RCVKDN"
     gene            complement(1797..3485)
                     /gene="ggt"
                     /locus_tag="PLZ75_07775"
     CDS             complement(1797..3485)
                     /gene="ggt"
                     /locus_tag="PLZ75_07775"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOS72331.1"
                     /translation="MKKPLIALLIVCLNIVSYSQDRITGHDFAGRSEIISMNGMAATS
                     QPLATQVALDILKKGGNAIDAAIAANAVLGLVEPTGCGVGGDLFAIIWSADKNKLFGL
                     NASGRSPRSLKLDYFKKNGYEFIPSYGPLPVSVPGCVDGWYEMHGMFGRLPMKDILQP
                     AITYSYEGFPVSEVIAWSLDRNTKALQEYPNIREVYMPGGKSPAKGEVFRNPLLAATL
                     EKIAKGGRDEFYRGSIARDIAAFMEKQGGFLTYDDLARHHSEWTEPVSTVYRGYEIWE
                     LPPNGQGIAALQILNILEGFNIAKMGFGSAEYIHHFTEAKKLAFEDRARYYADPLFSS
                     VPVEQLISKKYAAERRKLINPEKAARVYDAGLSEAGNTVYLTVADRHGNMVSLIQSNY
                     RGMGSGMCPTGLGFVLQNRGEMFSLSEGHPNVYAPGKRPFHTIIPAFITRNGRPWISF
                     GVMGGDMQPQGHAQIIINLIDFKMNLQEAGDAPRIYHTGSSEPTGELMTDGGILMLES
                     GFRTEVIQKLMSMGHRIQWNLGGYGGYQAIMRDEKNQVYFGASESRKDGQAAGY"
     gene            complement(3527..4234)
                     /locus_tag="PLZ75_07780"
     CDS             complement(3527..4234)
                     /locus_tag="PLZ75_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOS72332.1"
                     /translation="MDTIIEDLRFELKHNADEKTRISGERFFREEVKIHGLKSALVSS
                     IGREYFKKLPEKNKETVFGLCEELWKSGYLEESFIACNWAYCLRKEFTPDDFPVFEGW
                     VSKYVSNWASCDTLCNHSIGTFVEMYPSFVSDLKKWARSEKRWVKRASAVTLIIPARS
                     GKFLGDIFEIADILHSDPDDMVQKGYGWMLKAASEAHQQKVFRYVMRRKKSMPRTSLR
                     YAIEKMPSELKAQAMSR"
     gene            4559..5158
                     /locus_tag="PLZ75_07785"
     CDS             4559..5158
                     /locus_tag="PLZ75_07785"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HOS72333.1"
                     /translation="MRNTIILLLAAGISGMQFSCGKEENEPAIESKTFVIDVRNSSGW
                     KYFSFAEDDTIAVSDPSASTEWDLAFQRYRIRTNGGESGKGLGAAANTGQKGQAGFDA
                     VKSADDTFKFMDDGPVSIAVQQGYETFILNPELYTWFTMEQASQGTQIAPSDFVYIVR
                     TATGKYAKIWFKSYYSATNISGHITFQYKYQPDGSGNLE"
     gene            5200..7311
                     /locus_tag="PLZ75_07790"
     CDS             5200..7311
                     /locus_tag="PLZ75_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS72334.1"
                     /translation="MPSISLRRLTVFCLIILPSGLFAQKGSISGYVKGMNGEAIEAVT
                     LVIKNTTLGTVTDRNGFYKINNINAGTYIVRASLIGYETREKTVTVLEGENNADFGLD
                     ETNINLNEVVVTGTRSEKALKNVPVITQVINARRMIDLGITSVTGALQNMVPGLEIGQ
                     FGTRATVTMQGMDAKYVLFLVDGERIAGEISGDIDYSMLNLENIDRIEVIKGASSSLY
                     GSNAIGGVINIITKKIDEPFDARIYSRYSKYNEFTSGGGISFKRGMAGSRTSFNYSHH
                     DGYDITPETPFEWTQNPYNSFSVNQKFEITPSPDFSFSPYFGYYRFERGNVSARPVHD
                     LYTDFKAGLKGEYYFREHSLDFSYYRDRYNTFNVFELLDNLKDTASYDIIQTVRTQGN
                     FKISEKNSLTAGLEYNHEKLLSDRIEEGIKNAGEAVFYVQEDISPGKRWNIIAGVRVS
                     YHSNYGFNAAPKISLMFKSGFGNLRASAGTGFRSPALKELYMNFDHFGEWYLMGNPEL
                     GPESSLYVSGSYEFSRPWNNSSVSVYHNSLSNMITDRWLPDTVRLTRQYENVASASVS
                     GIDFLSKQKIFKGLWISVGYNYVQSHDNETGLQLYGTTKHSGNISSDYNFRKKNYSLT
                     VQLLCKLTGEKFYEITSEGIDRDRPFSTWRLTLSQEYKWLRLTTGVDNIFNLVIPYNN
                     NYISPGRRFFIGTNIDFGKIR"
     gene            complement(7399..>8074)
                     /locus_tag="PLZ75_07795"
     CDS             complement(7399..>8074)
                     /locus_tag="PLZ75_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72335.1"
                     /translation="SRRAMEMRDMTADISWETTGSELIALLRESFEIKENKPFFNRAQ
                     RRSSFQWGIYSSTDSSGFITFSIMPVSGEESPLSVFTSRGRARSRLESLAGSYNLCRK
                     LTGLYESEGACFQYHVGICRGACCGQESPSDHNARALKAMEEFVFSARNFFLIDRGRS
                     DEEKSAVRIINGKYSGYGYFDINDMGFGLEAVHDCIKPFPDNRDIQVILKQYLKDNKP
                     GKIIEF"
BASE COUNT         2204 a         1794 c         1810 g         2266 t
ORIGIN      
        1 tgcgttatac ttccggagta agtcttattg ttgccggttc gggagatgtc atcggcaggc
       61 ttaaaaacag agtccttgac ctggaactgg cggacaggat tttatttctt gatatgatgc
      121 catgggagga gctgatgaag tatacccgtt cggctgatgc agggctcagc ctcgacaagg
      181 ataccaatat gaactacagg ttcagccttc cgaacaagct ctttgattat atttcggccg
      241 gaataccggt tatagccgga aatctgccgg aagtagcggg tattataaaa aaatatgact
      301 gcggtataat tattcctgaa ataactcctg aggcaatagc tgaagcggtt gaaacactta
      361 aaaatgacag ggaacttctg aacaaactca ggcggaatgt tgtttatgca tcagaagacc
      421 tttcctggga gaaggaatca gaaaaagtaa aaaagtttta tgaaagagtt tttaaagaaa
      481 aatagcctgt tggtaatatt tacaatatcc ggcctcgccg gcggatttct ttactggaaa
      541 tttgtcggat gcctttcggg tacctgtccg attaagtcgg tatggtactg gagcaccctt
      601 tacggcggac tgataggata tcttgccggc agcatacttc ttgacgtaat acgtaaattc
      661 agtaaaagga aaggtattaa cgatagttct gaaaactgaa ttcccccagg aaacaaaatt
      721 cattccggca attggtctcc cgactgtttc tgtcaatttt cagaccggcc gtaaccgggt
      781 ttcagacaac taatctgacg tgttagttgg caacaggtgt ctgacgaaac acagaaacgg
      841 gtttgcactc tgaaaaagaa attttaattg catgacacac atttctgaaa cgtttgaaag
      901 actccacgta taatgttgct ttaatgttga ttacatattt tttcagcatt acttttttta
      961 ctaaaaacaa ttgattatga caacaggaaa aaagctctat tactctgcat ttgcccttct
     1021 gggagcaacg ctattgcttt tcagcagttg caaaaaagag aatgtccccg atccgtccac
     1081 tgtagatgct gatggcaatg tttaccccac tgtggtcatt ggcgggcagg agtggttcgc
     1141 cgagaacctt cgcaccacca agtacaacga tggtacctcc atacccaacg taactaaatc
     1201 ctgggaaatc cagtccacgg atgcctattg ctggtataat aacgaaccgg acaatggcaa
     1261 caaatacggt gctctttata actggcttgc tgtgaagaca ggcaagctct gccccaccgg
     1321 ctggcacgtg cccagcgttg aggagtggca ggctcttatg gattatgcgg ctcctgataa
     1381 cgctgtcaag ctaaaaagta ccagtgggtg gtataacaac ggcaacggca ctgatgatta
     1441 cggcttctca gcccttccgg gcggctaccg cgacgttttc ttcaaagatc tgggcctcta
     1501 cggccactgg tggaccagtt acgggttcgt tcatgaacaa gtctgggaat tggccgagag
     1561 ccgggagatg tattggggct acaagacggt gtaccgcgcc ctcgtatatt cgtccatagg
     1621 gtattcagtc cgttgtgtaa aggataacta gattatttgg ttatttactg cgtagctatc
     1681 gattttttta ttcaggctaa ttacgcaact tcggaattgt atcgcccgtt tggagcgatt
     1741 tcgtcatgca accaaatcta aactcgcgct tccgcacttg ccataccccc ggccgttcag
     1801 taacccgctg cctgtccgtc ttttcttgat tcggaagccc cgaagtaaac ctggttcttt
     1861 tcgtctctca taatggcctg gtagccgcca tagcctccaa ggttccactg tatcctgtgg
     1921 cccattgaca tcagtttctg aatcacttcg gttctgaaac cgctttccag cataagaatc
     1981 ccgccatcgg tcataagctc cccggttggt tccgatgatc cggtgtgata gattctgggt
     2041 gcgtccccgg cttcctgcag attcatctta aaatcgataa ggtttattat tatctgtgcg
     2101 tgcccctgtg gctgcatatc cccgcccatt acgccgaaac ttatccaggg cctgccattg
     2161 cgggttatga aagccggaat gatggtatgg aagggtcttt tgcccggtgc ataaacattc
     2221 ggatgccctt ccgacaggct gaacatctca cccctgttct gaagcacaaa gcccagtcct
     2281 gtgggacaca tacctgatcc cattcccctg tagttgctct gaatgagaga gaccatattc
     2341 ccatgccggt ctgcaacagt aagatacaca gtgtttcctg cttcggacaa tccggcatcg
     2401 tatacccggg ctgctttttc aggattgatg agtttgcgcc tttcagcagc atatttcttt
     2461 gaaatcagct gttctacagg tacggaactg aaaaggggat cagcatagta ccttgctctg
     2521 tcctcgaatg caagtttttt tgcttcggtg aaatgatgaa tgtattcagc ggatccgaat
     2581 cccattttcg ctatattgaa tccttccagg atgttcagga tctgaagggc tgcaataccc
     2641 tgaccgttcg ggggcagttc ccatatttca taacccctgt agactgtgct taccggttct
     2701 gtccattcgg aatgatggcg ggcaaggtca tcataagtga ggaaaccgcc ctgtttctcc
     2761 ataaatgccg caatatccct ggcaatgctt cctctgtaaa actcgtccct tccgcctttt
     2821 gcaatttttt ccagtgtggc agccagaagg ggattcctga acacttcacc ctttgccggt
     2881 gattttccac caggcatata tacttccctg atattgggat attcctgcaa tgcttttgtg
     2941 tttctatcaa gggaccaggc tattacttcc gatacgggaa aaccttcata ggaataggta
     3001 attgcaggct ggagaatatc tttcatggga agcctgccaa acatgccatg catctcatac
     3061 cagccatcca cgcagccggg gacagatacc ggcaatggtc cgtatgatgg tatgaattca
     3121 tagccatttt ttttgaaata atccagttta agggatcgtg gtgaccgtcc gctggcattc
     3181 aggccgaata atttattttt gtccgcgctc catattatgg caaacagatc cccgccaacc
     3241 ccgcagcccg ttggttcaac cagtccgagt accgcgttgg ccgcaatggc agcatcaata
     3301 gcatttccgc cttttttcag aatgtcaagg gcaacctgtg ttgccagcgg ctggcttgtt
     3361 gcagccatgc cattcatgga aataatttct gacctgcctg caaaatcatg gccggttatc
     3421 ctgtcctggg aatatgaaac aatattcagg caaactatta acagtgcaat taacggtttc
     3481 ttcatttcaa tggttttgat ttcaaaatta agaaaatcag gattgattac cttgacatgg
     3541 cctgtgcttt cagttctgaa ggcatctttt ctattgcata cctgagtgaa gtccgcggca
     3601 tgcttttctt ccttctcata acataccgga aaactttctg ctgatgcgcc tcactggccg
     3661 ctttcagcat ccagccgtaa cctttctgaa ccatgtcatc agggtctgaa tgaaggatat
     3721 cggctatttc gaaaatatca cccaggaatt taccgcttct tgccggaata ataagcgtta
     3781 cagctgaagc cctttttacc cagcgctttt cagaacgggc ccatttcttc aggtcagaca
     3841 caaaggacgg atacatttca acaaaggtcc ctatgctgtg attgcatagt gtatcacatg
     3901 atgcccagtt gcttacatat ttactgaccc atccttcaaa tacagggaaa tcatccggag
     3961 taaactcttt ccttaaacag taggcccagt tgcaggcaat gaatgattct tccaggtaac
     4021 ctgatttcca tagttcttcg caaagaccga agaccgtttc cttgtttttt tccggcagtt
     4081 ttttgaaata ttccctgccg atggaactga caagtgccga ttttagtcca tggatcttta
     4141 cttcctccct gaagaacctt tctccggaaa tccgggtttt ttcatccgca ttatgcttta
     4201 gttcgaacct taagtcttct attatagtgt ccattttaac cggtatttat ttttctgaaa
     4261 gttagtattt tttgcctgta agctgtaagt ttgccgggac atggtgactg ataaaccccg
     4321 gggaaaaaga tataaatagt taaagatgtt aaactttcct gaaaaatatt ctgttatgag
     4381 agaattttct aaaaccataa aaaaagcaaa aaaaagtatt ttccttttgt gggtttcatt
     4441 gccattaagc ctgatgagac cagtaatttt gtacttttat cgcggaacaa tttccggatg
     4501 attttgttgc aatattaacg tttggaatca aatcaggata taatatgaat tttatgacat
     4561 gagaaataca ataatcttat tgctggcggc aggtatttcc ggaatgcagt tttcatgcgg
     4621 caaagaggaa aacgagcctg caatcgaatc aaagacattt gtaatagatg taagaaattc
     4681 ctccggctgg aaatatttca gctttgcaga ggatgatacc atagccgtat ctgacccttc
     4741 tgcctccact gaatgggacc tcgccttcca gcgctacagg ataagaacca acgggggaga
     4801 atccggaaag gggcttggag ctgcagcaaa caccggtcag aaaggacagg caggattcga
     4861 tgccgtaaaa tcagcagacg atacttttaa attcatggat gatggtccgg tttcaatagc
     4921 ggtgcaacag ggctatgaaa catttattct caatcctgaa ctctatacat ggtttacgat
     4981 ggaacaggct tcccagggaa cccagatagc cccttctgat tttgtatata ttgtaaggac
     5041 tgcaaccggt aaatatgcaa aaatctggtt caagagctat tatagtgcaa caaacatctc
     5101 aggccatatc acatttcagt acaagtatca gccggatggt tccggaaatc ttgagtaaag
     5161 atcaggataa gtttcagtaa aagttatatg accagaccaa tgccgtcaat ttcattgaga
     5221 cgactgacag ttttctgctt gattatttta ccctccggcc tttttgccca gaaagggagc
     5281 atttcgggtt atgtgaaagg aatgaatggt gaggcgattg aagctgttac cctggtaata
     5341 aaaaacacca ccctgggaac ggtgacagac aggaacggct tttataaaat aaacaatata
     5401 aatgcgggta catatattgt ccgcgcctca ttaatcggat atgaaaccag ggaaaaaacg
     5461 gtcactgtcc tggaaggtga aaataacgca gatttcggac tggatgaaac aaatataaac
     5521 ctgaatgagg ttgttgttac cggaacaaga tcagaaaaag ctttgaaaaa tgtaccggtg
     5581 atcacacagg ttatcaatgc gcgccggatg atcgatctcg gaattaccag cgttaccggg
     5641 gcacttcaga acatggttcc cggcctcgag atcggccagt ttggaaccag ggccactgta
     5701 accatgcagg ggatggatgc caaatatgta ctcttccttg ttgacgggga gagaattgcg
     5761 ggagaaatca gcggagatat tgactattca atgctgaatc tggagaatat tgacaggatt
     5821 gaagttatta aaggcgcttc atcatcatta tatggatcaa atgccatcgg aggggtaatc
     5881 aatatcatca caaaaaagat tgatgaacct tttgatgcca ggatttattc cagatattca
     5941 aaatacaatg aatttacttc aggaggggga ataagcttta aaagaggtat ggcaggatca
     6001 cggacaagct ttaattacag tcatcatgat ggctatgata tcacgcctga aactcccttt
     6061 gagtggactc agaatccata taattcattc agtgtcaatc agaaatttga aataacaccg
     6121 tccccggact tttccttttc cccttatttc ggatattacc gctttgagag ggggaatgta
     6181 agtgcacggc ctgtacatga tctctataca gattttaaag ccggactgaa gggtgagtac
     6241 tatttcaggg agcattcatt ggatttttcc tattaccgcg accggtataa tactttcaac
     6301 gtatttgaac tgctggacaa cctgaaggat acggcttcct acgatattat tcagacagtg
     6361 aggacacagg gcaactttaa gatttccgaa aaaaatagtc tgacagccgg tctggaatac
     6421 aatcatgaaa agctattgtc ggacaggata gaggaaggga ttaaaaatgc cggggaagca
     6481 gtgttttatg ttcaggaaga tatttccccg ggaaagcggt ggaacataat tgcgggcgtg
     6541 agagtaagct atcacagcaa ctatggattt aatgcggctc caaaaatatc gctgatgttt
     6601 aaatcgggct ttgggaattt acgggcttcc gccgggacag gtttccgttc tcctgcctta
     6661 aaggaactgt acatgaattt tgatcatttc ggggaatggt atttaatggg taatccggaa
     6721 ctgggacccg aatcatcact gtatgtgtca ggatcgtatg aattttccag accatggaat
     6781 aattcatcgg tttcagtata tcacaactcc ctttccaata tgattaccga cagatggctg
     6841 cctgatactg tccgcctgac ccgtcagtat gagaatgtgg ccagcgcatc ggtgtcggga
     6901 attgattttc tgtcgaaaca gaaaattttc aaaggactgt ggatcagcgt gggttacaac
     6961 tacgtgcagt ctcatgacaa tgagacaggc cttcagttat acggaaccac aaaacactcg
     7021 ggaaatattt catcagacta taatttccgc aaaaagaatt attcattgac ggttcagctt
     7081 ctgtgcaagc tcacgggtga gaaattttat gaaataacca gcgaaggaat tgacagggac
     7141 aggcccttca gcacctggag gcttactctt tcacaggaat ataaatggct gaggcttaca
     7201 accggggttg acaatatttt caacctggta attccgtata acaataatta tatttctccc
     7261 ggaaggaggt tttttatagg aacaaatatt gatttcggga aaatcaggta gaatcaccat
     7321 gaaccgggcg gaagcggatt ttccccggca gtgattttat tccggggcat agcggatctt
     7381 tctgaagcag aataatactc agaattcaat aatctttccc ggcttattat ccttcagata
     7441 ttgttttagt attacctgta tgtccctgtt gtcgggaaat ggtttaatac aatcatgcac
     7501 tgcttccagg ccgaacccca tgtcattgat atcaaaatag ccgtaacctg aatattttcc
     7561 gtttattatc ctgactgcac ttttttcctc atcgcttctt cctctgtcaa taaggaaaaa
     7621 attgcgtgct gaaaacacaa actcctccat ggcctttaag gcacgggcat tgtggtctga
     7681 cggggattcc tgcccgcagc acgcacccct gcatataccg acatgatact ggaaacaggc
     7741 tccttcgctc tcatacagcc ctgtcagttt ccggcaaaga ttatagctgc ctgcaaggga
     7801 ttcgagcctt gacctggccc ttcccctgga agtaaagacg gacagtgggc tctcttcacc
     7861 tgataccggc ataatgctga aggttataaa tccgctgctg tcggtagagg aatagattcc
     7921 ccactggaaa gaagatctcc tctgtgcccg gttaaagaat ggcttgtttt ccttgatttc
     7981 aaaagattcc cttaacagtg caataagttc gctgccggtt gtttcccagc tgatatctgc
     8041 tgtcatatca cgcatttcca tagccctccg ggaa
//
LOCUS       DAOSPX010000134         8044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8461_length_8044_cov_5.161597, whole genome shotgun sequence.
ACCESSION   DAOSPX010000134 DAOSPX010000000
VERSION     DAOSPX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8461_length_8044_cov_5.161597"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..665)
                     /locus_tag="PLZ75_07800"
     CDS             complement(204..665)
                     /locus_tag="PLZ75_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOS72336.1"
                     /translation="MADQNIIRILTENGLRVTPQRTAILEAIMNLHTHPTADDIVKHL
                     RLTYPNIPYGTVYKILDRFIKTGIVQKVKTDGDLLRYDAVKVRHHHLYSSVNDSIEDY
                     YDEELDNILSDYFEKKKIPGFLVEDFKLQIIGKRENSPENNSPLNTKINNQ"
     gene            complement(895..1584)
                     /locus_tag="PLZ75_07805"
     CDS             complement(895..1584)
                     /locus_tag="PLZ75_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72337.1"
                     /translation="MKIKEIFRIAMAGIILLAVTPDTAGQTFSVTFDLHDMCRKNQIT
                     TYNRQTDCFTENGTKIIRLSGNNGYGIAWIRGMEFADGIIEVDIRAGSEIQHFAAGLA
                     FHGLGSDTLDAVYLRRSVSPEDKKTGSHRLFYTSLPAPASEESNNHFLILYKKAFSPR
                     PDAKGWIHLRISVQGDQISAFAGRNDDPFLVVERQGRRTGGRIGFWTGDMSGGDFANL
                     RISTGPVKATQ"
     gene            1712..2347
                     /locus_tag="PLZ75_07810"
     CDS             1712..2347
                     /locus_tag="PLZ75_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS72338.1"
                     /translation="MKEYTDNEIIECLRARQSYVVRFLHDRYLPMIRLMITGMGGTAE
                     DAMDIFQDGLMIMIEKLDNREFTLTCKFKTYLYCVCENLWKSILTKRKAAQNYFLRKV
                     DDEDDDFTESMDGSLYESIFHAAFETLDPAARNILKLYWEEVSPREIAEKLGYSYAYV
                     RKKKCEAQAELILKVKKHPDYLRIMKHKDPSDDRMPSSQNNIQSDMAISGR"
     gene            2344..3390
                     /locus_tag="PLZ75_07815"
     CDS             2344..3390
                     /locus_tag="PLZ75_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72339.1"
                     /translation="MKPEDRYMDKGESPGRKQDHNDDPEDAVLLSPGDNVLLGKINDY
                     FKARFDIEDVKNDPEYRKTDESVKSFISGLESGERDGRENEIRRFIRDSIYDDGRNAG
                     PDPEISRIRQESSHRQIEGITAEWVREWHERKRMEPQEGISEKQKEIRDFVIDSLQKE
                     EGGGVLYELPQASRGHRGMKVLFPWAAIAAAAAIAVLFLIRGLMTASDPERLFSRYYE
                     PYYAASLVTRSSGQGEDETLRSALGYYRNHNYRMAAVEFSKALLTASAPDMPVFYLGV
                     TLIELEEFERAFMILEPLTVQPGEFLKDAQWYLGLLSVRTGKTDKARECFESLAGDPG
                     FYSVRAGKILRLLK"
     gene            complement(3348..6047)
                     /locus_tag="PLZ75_07820"
     CDS             complement(3348..6047)
                     /locus_tag="PLZ75_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOS72340.1"
                     /translation="MHAGDTDSLFLRFSSLYQAGNLLEAEKTLSVILEPGTQSDESEL
                     IAAYNNLGVVNQMLGRYDKALEFFSMAESLAIHRKDYIKNLPDIYSNKGIIHSIRQSF
                     ETAIIYFEKSIRVYNSLDLTDKTILNSLASVYLNAGIAFRNAGDYPKALDFFNNSLRI
                     KSEHGFQGTGLVYMNMAKTWTKLKNSRKAEEFYKKSIEYLYSAYGGNYFRLAEVYFDY
                     GIFLDSEGRFAEAREIHEKALSICLDNYGTKHTLVALAYKNMGDHCFRQSAFRESLEY
                     YQKSLIAVVKNFNDNDIYSNPSIDSVLFDVRLLDNLKSKSKSLEYLADEMDDNRLKLK
                     TLRKSLETAELALELSNRLKNNHFSEESRLYISENEKATCLHAIHVAHILYSMTGEKA
                     VLEKMYSVARNEKASILRNQIIENELFFLAGIPDSLRDKRNRLSENIAALHKMIADES
                     LKMMPDSKKISFWKDALFDMNINYEKTISDINTSFPQFRNLLEKTGPVTLSSVRHKLR
                     SNETILDYILSEPDDGKERDLYIFIVTGKKLQFLKLKADSMFTEYARLLGNYSRSRTA
                     ADFGQLTGALNYMYNLLVAPAEKYFAGKKLIIIPDEEISWLPFDAFLPEKPCPVRGHY
                     DGLKYLINDYTVSYYFSASMIGERTRRGRLSGKVFAFYPVYNESQAGDESYAGLPDAA
                     TEIESLLKVFRGEKYLGSNAGITNFLKVINKPVIFHLAMHLTSDSLNSRYSFLLFDTS
                     SDSVSDGKLYNYEISSLDMKSPMVVISSCNSGTGTLRQGEGLMSIARSFRLAGASSVV
                     TTAWEINDETSARIISRFYYYLSRGKFKDEALRLSKLDYLREMPPAFSGPYYWAAYEI
                     TGDNSPVVIPLKPFAPAALAILPAAAAVIFYLRRRRIFPARTE"
     gene            complement(6224..6412)
                     /locus_tag="PLZ75_07825"
     CDS             complement(6224..6412)
                     /locus_tag="PLZ75_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72341.1"
                     /translation="MKNKNKSSRTERNFSTLVAGLRKHQILDTARMIHVRGGEGEGSG
                     YEPIIIIPPKKKDPERPS"
     gene            complement(7132..7207)
                     /locus_tag="PLZ75_07830"
     tRNA            complement(7132..7207)
                     /locus_tag="PLZ75_07830"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7172..7174),aa:Lys,seq:ctt)
     gene            7335..>8044
                     /locus_tag="PLZ75_07835"
     CDS             7335..>8044
                     /locus_tag="PLZ75_07835"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOS72342.1"
                     /translation="MINVRIVSPDESVSFPAEKGRRLLDILRERGFDIYTPCGGRGRC
                     GKCLVSIRGTGNVLSCSYYPDRDIEVTLPEKRVADILVSQTEFLEDCTFSPPSSLLSA
                     RPYGVAIDIGTTTVAMYFMNLLTGSLEKIASFLNPQGIHGADVISRITYCQEHENGLG
                     ELQQIIINAINNELELFLQQKDLQSGSIERVIVSGNTTMLHLLLGEDPVPIALAPFRP
                     RFTGKQRTEGSSSGLNINP"
BASE COUNT         2211 a         1658 c         1851 g         2324 t
ORIGIN      
        1 ctcttcaagt tctgtagtga ctgcaacttt catcgggccc ttaactattt ttgcgccgat
       61 ccagtactga tagtaagcca gccattcatc ggccagcgct ttgttcagga gattgatcag
      121 ttcgtcaaca tccattttta caattgctct tccaatttct gccataatag tcggattttt
      181 aagaattata ccatcaaaat tagttattgg ttattgattt ttgtgttcag gggagagtta
      241 ttttcaggac tgttttcccg ttttccgatt atctgcagct taaagtcttc aaccaaaaaa
      301 ccgggaattt ttttcttttc aaagtaatct gacaggatgt tatccagttc ctcgtcataa
      361 taatcttcaa tggaatcatt gacagatgaa taaagatgat ggtgccgtac ttttaccgca
      421 tcgtatctta acaggtcacc gtcggttttt actttctgga ctatgcctgt tttaatgaat
      481 ctgtccagaa tcttatagac agtaccgtat ggaatattgg gataggtgag ccttagatgc
      541 ttaactatat catctgcggt gggatgggta tgcagattca tgatagcctc caggattgcg
      601 gttctctgag gggttaccct gagtccgttt tccgtaagta tcctgatgat gttctgatct
      661 gccaatgctt taatttttta ataactgtca ggttctttga taaatggaat tataatcaaa
      721 ttctgttctg aaagccatgg ctttaagatt aattatgcca ggttattccg cagaagataa
      781 ttatcatttt tagagaatga ttctccggag agaatagttc tcttttgaga aaacaaagct
      841 aacaagattt cgtgaataaa aaaaatcaga aaatataaat ctgattaaag gaaatcactg
      901 tgtggctttc acaggtccgg tgcttatcct gagatttgca aaatctcctc ccgacatgtc
      961 tccggtccag aatccgatcc ggccgcctgt acgtctgccc tgcctttcaa cgacaaggaa
     1021 gggatcatca tttctgccgg caaatgccga tatctgatct ccctgaacgg aaatcctcag
     1081 atgtatccag ccctttgcat caggccgggg gctgaatgcc tttttgtata aaatgagaaa
     1141 atgattattt gattcttcag aggcaggtgc cgggagggat gtataaaaca gtctgtgcga
     1201 tccggttttt ttatcttcgg gagaaacgga tcttctcaga tacacggcat ccagggtatc
     1261 actgcccagt ccgtgaaatg ccagacctgc agcgaaatgt tgtatttcac tcccggcccg
     1321 aatatccact tcaattatac catcagcgaa ctccataccc cttatccacg cgattccata
     1381 gccattgttc cctgacaggc ggataatttt tgtgccgttt tcggtaaaac agtctgtctg
     1441 ccggttgtat gtcgttatct gatttttccg gcacatatcg tgcaggtcaa atgttactga
     1501 aaaggtctgg ccggcagtat ccggcgttac tgcaagcaat atgatgcccg ccatcgcgat
     1561 gcggaaaatc tctttaattt tcatgattca aatccatggt taaatatgaa tgcctgatga
     1621 tttgttacct tctcctgcaa taaattttca ttttcagcca aaattcccta tttttgatta
     1681 atggttttta ctgccgaaag agtaaggctt tgtgaaagaa tacacggata atgaaataat
     1741 tgaatgcctg cgtgcccggc agagttacgt ggtacgattc cttcatgacc gttatttgcc
     1801 catgatccgg cttatgatta ccgggatggg cggcacagcg gaagatgcca tggatatttt
     1861 tcaggatggt ctgatgatca tgattgagaa acttgataac agggagttta cattaacttg
     1921 caaattcaag acctatcttt actgtgtctg tgaaaatctt tggaaatcaa tattgacaaa
     1981 aaggaaggct gcacagaact actttctgag gaaggtggat gatgaggatg acgattttac
     2041 tgaaagtatg gatggcagcc tgtatgaatc cattttccat gctgcatttg aaacgcttga
     2101 tccggcagcc aggaatatcc tgaagcttta ctgggaagag gtttctcccc gcgaaattgc
     2161 agagaaactg ggatattcct atgcctatgt gagaaagaaa aaatgtgagg cccaggcgga
     2221 acttattttg aaggtgaaga agcatccgga ctatctgagg ataatgaagc ataaagaccc
     2281 ttctgatgac aggatgccgt catcacagaa caatattcag tcagatatgg ccatcagcgg
     2341 cagatgaaac cggaagacag atatatggat aagggagaat ccccgggtag aaagcaggat
     2401 cataatgatg atcctgaaga tgctgtattg ctttcacccg gcgataatgt gctccttgga
     2461 aagataaatg attatttcaa agcccgtttt gacattgaag atgttaaaaa tgatcctgaa
     2521 tacaggaaga cagatgaatc tgtaaaatcc tttatttccg gtctggaatc cggtgaacgg
     2581 gacggaaggg aaaatgaaat acgaagattt atcagggaca gtatttatga tgacggtcgt
     2641 aatgccggac ctgatcctga aataagccgg atcaggcagg aaagcagtca taggcagatt
     2701 gagggcatta ctgccgagtg ggtccgggaa tggcatgaaa gaaagcgcat ggaacctcag
     2761 gaagggataa gtgaaaagca gaaggagatc agggactttg tcattgattc acttcagaaa
     2821 gaagaggggg gcggggtact ttatgagctt ccccaggcaa gccgggggca caggggcatg
     2881 aaagttctgt ttccgtgggc agcaattgct gcggcagctg ccattgccgt tctttttctc
     2941 atcaggggcc tgatgactgc cagtgatccg gaaagactgt tcagcagata ttacgagccc
     3001 tattatgcag catcgcttgt taccagaagt tccggacagg gggaagatga aacccttaga
     3061 tctgcccttg gctactacag gaatcataat taccggatgg ccgcggtgga attttcaaag
     3121 gcactgctta cggcatctgc tcctgacatg ccagtatttt atctgggtgt aacacttatt
     3181 gaactggaag aatttgagag ggcatttatg atccttgaac cactgacggt tcaaccaggg
     3241 gaatttctga aagatgcgca gtggtatctg ggtctgctgt ccgtcaggac gggaaaaacg
     3301 gacaaagccc gggaatgttt tgaatcactt gcaggagatc ccggatttta ctccgtccgg
     3361 gccggaaaga ttctccgtct tcttaaatag aaaatcactg cagctgcagc cgggaggatt
     3421 gccaatgctg cgggggcaaa gggttttaac gggatgacaa ccggtgaatt gtcgccggta
     3481 atttcatatg ctgcccagta atatggcccg gaaaatgcgg gaggcatttc cctcagataa
     3541 tcgagttttg acaggcggag agcctcatcc ttgaattttc ccctggagag atagtaatag
     3601 aaacgggaaa ttattcttgc acttgtctcg tcgttgattt cccaggcggt tgtgaccacg
     3661 gatgatgctc cggcaagcct gaaactccgt gcaatgctca tgagaccctc accctgacgg
     3721 agtgttcccg ttccggaatt acatgaactg atcactacca tcggggattt catatccagt
     3781 gagctgatct catagttgta cagtttaccg tcagagacac tgtcagagct cgtatcgaag
     3841 agcaggaagg aatatctgga attcagtgaa tctgaagtta aatgcatggc aagatggaag
     3901 attaccggtt tattgattac tttcaggaaa ttcgttatgc cggcattgct gcccaggtat
     3961 ttctctcccc tgaaaacctt taataatgat tcaatttcag ttgcggcatc aggaagcccg
     4021 gcgtaggatt catccccggc ctgactctca ttgtatacag gataaaaggc aaaaaccttt
     4081 cctgacaatc tgccgcgccg ggttctttcc ccgatcatgg aggccgaaaa gtaatacgac
     4141 acggtatagt catttattag atatttcagc ccgtcatagt gaccccggac cggacagggt
     4201 ttttccggca gaaaggcatc aaaaggcagc catgatattt cctcatcagg aattattatc
     4261 agctttttcc cggcgaaata tttttcagca ggggcaacaa gcagattgta catgtaattc
     4321 agggcgccgg taagctgacc gaaatcagct gcagtcctgc tgcgggaata attccccagc
     4381 agccgggcat attccgtaaa catgctgtct gcctttaatt tgagaaactg cagttttttc
     4441 ccagtaacta tgaatatata cagatcccgc tctttcccgt catccggttc cgagaggata
     4501 taatccagta ttgtctcgtt acttcgcaat ttatggcgga cggatgaaag agtgaccggg
     4561 ccggttttct caaggagatt ccggaattgc ggaaatgagg tattaatatc cgaaattgtt
     4621 ttttcatagt ttatgttcat gtcgaaaaga gcatccttcc agaatgatat ctttttactg
     4681 tccggcatca ttttcaggga ttcatcagct atcattttat gtaatgctgc aatgttctct
     4741 gaaagcctgt tgcgtttatc ccttaaagaa tccggaatac ctgcaaggaa aaacagttca
     4801 ttttcaatga tctgatttct cagaatggaa gctttttcgt ttcgtgccac gctgtacatt
     4861 ttttcaagca cagccttttc cccggtcata ctgtaaagaa tgtgcgccac atgaattgca
     4921 tgcaggcatg tggccttctc attttctgaa atataaagac ggctttcttc actgaaatga
     4981 ttgtttttca gacggtttga caattccagt gcaagttcgg ctgtttccag gctttttctg
     5041 agtgttttca gtttaagcct gttgtcgtcc atttcatctg caagatattc cagcgatttt
     5101 gacttgcttt taagattgtc aagaagccgc acatcgaaaa gcacggaatc aattgaagga
     5161 tttgaataaa tatcattgtc gttgaaattt ttcacgacgg ctatcagaga tttctgataa
     5221 tattccagcg attccctgaa ggcggactgc ctgaaacagt gatctcccat gttcttgtat
     5281 gccagggcaa caagcgtatg tttcgtcccg tagttatcca ggcagatgct aagtgccttt
     5341 tcgtggattt cacgggcttc tgcaaacctt ccctcagagt caaggaaaat cccgtaatca
     5401 aaatatactt ccgccagtct gaaatagttc ccgccgtatg cggaatacag gtattcaata
     5461 cttttcttat aaaattcttc agctttccgt gagtttttca gtttcgtcca ggtttttgcc
     5521 atattcatgt aaacaagccc tgttccctga aatccgtgtt ctgactttat tctgagactg
     5581 ttgttaaaaa agtcaagggc cttggggtaa tctccggcat tacggaaggc tattccggca
     5641 ttcagataaa ctgaagccag tgaattgaga atggttttat ccgtcaggtc cagactgtta
     5701 tataccctga tgctcttttc aaaatatatt attgcagttt caaaggactg cctgattgaa
     5761 tggatgatac ctttattgga ataaatatcc ggcaggtttt taatataatc cttcctgtgg
     5821 attgccaatg attctgccat gctgaagaat tccagtgcct tatcatatct tcccagcatc
     5881 tggtttacaa cacccagatt attataagca gctataagtt ctgattcgtc actttgtgtt
     5941 cccggttcaa gaatgacgct gagtgttttt tctgcttcaa gaagattacc ggcctgataa
     6001 agggatgaaa atcgcagaaa aagagagtca gtatcccccg catgcacaaa tgaaaaaaat
     6061 gcaagtaaat gaattaaagg aataaatcct gttcttatca atactctaag tctgaaatct
     6121 gtaagagttg ttgatcgtta aataatttgt gcaaattaat taaaaaaata aaacagacaa
     6181 gaagagcatc ctttatatgg cgggatgctc ttttctgaaa ttgtcaggac ggacgttccg
     6241 ggtccttttt ctttggcggt ataataataa tgggttcata tccgcttcct tccccttcac
     6301 cgcccctgac gtgaatcatt cgggcggtat caaggatctg atgttttctt aatccggcaa
     6361 ccagtgttga gaaatttctt tctgtacgac ttgatttgtt tttgtttttc atgattttat
     6421 ggttttaaat taaacatttc atcaattaat atgaataagc ttttaaatgt tacttttttt
     6481 gccctgatcg cggaatccgg ctgataccgt taacggtatc aaaaaagtat tatccggaag
     6541 caataagcac tgtggttatt ccggttccgg cccggaagat tcctgttcat tcaggcggta
     6601 acaatttctg agtaaaactt attaaactcc ggattggaat taatcagaga acctggtacg
     6661 ggaactgtca agattaagaa tcatataaaa tatcataaaa gccgtgggta taaaatatct
     6721 tattaaatgc cggccgcgtg ccggcggaag tcataccgtc cacagggagg gctgcccttt
     6781 tctggccgga aacggaaatg aaaaatatct gggttgtttc gaataccccg ggaaagcact
     6841 ggaggaaggc agaaagtatt atgaaagagc ggatttctgt gtgttttgca tgaaagaaaa
     6901 atgcggaggg gagaatttaa aaaacacagt ccctgacccg ctgaagcagg ttccagcctc
     6961 agcatgcatc acagattccg gtccgggaaa tatcctgatc tgttgtatta attgaaaaag
     7021 aagtccggtt accgcagggc tgatgccggt ttaaatccgt ttctgggtat tcgtttgtat
     7081 accggaaaaa aaaggcagtt tacaagggat cctgtaactg cctttaaagt gtggtcggcc
     7141 cggagaggct cgaactctcg accccatgat taagagtcac gtgctctacc agctgagcta
     7201 cgggccggaa aaacgatgca aaagtataaa atatattctt ccgctgtgca cttatttgcg
     7261 ggaaatctga aatgattgat ttaagtaatt atatttgagt caggcaagag ttagcattaa
     7321 taaaacggaa gctggtgatt aacgtcagaa ttgtttcgcc ggatgaatca gtgagttttc
     7381 ctgcagaaaa gggcaggaga ctgctggata ttctcaggga gcgcggcttt gatatataca
     7441 ccccctgcgg cggcaggggc agatgcggaa aatgcctggt cagtatcagg ggaacgggaa
     7501 atgtcctctc atgttcatac tatcctgaca gggatattga agtgactctt ccggaaaagc
     7561 gtgtggctga tattctggtg agtcagacag aatttcttga ggactgtaca ttcagtccgc
     7621 cttcttcatt attaagtgca aggccatatg gcgtggcaat tgatatcggc acaacaactg
     7681 ttgcaatgta ttttatgaat ctgctgaccg gaagcctgga aaaaattgca tcttttctga
     7741 atccccaggg tattcatggc gctgatgtga tctccaggat cacctattgc caggagcatg
     7801 aaaacggtct tggggagctt cagcagatta taatcaacgc cataaataat gaactggagc
     7861 ttttcctgca gcaaaaggat ttacagtccg gcagtatcga gagggtcatt gtatcaggaa
     7921 atacaacaat gcttcatctg ctgctggggg aagacccggt acctattgca cttgccccct
     7981 tcaggccacg gttcaccgga aagcagcgta ctgaaggcag ttcttccgga ctgaatatca
     8041 atcc
//
LOCUS       DAOSPX010000135         7918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8627_length_7918_cov_5.579041, whole genome shotgun sequence.
ACCESSION   DAOSPX010000135 DAOSPX010000000
VERSION     DAOSPX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8627_length_7918_cov_5.579041"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..986)
                     /locus_tag="PLZ75_07840"
     CDS             complement(288..986)
                     /locus_tag="PLZ75_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS72343.1"
                     /translation="MKRLLFLVLVFNIIVASGQNDSVSICGKVADFDDRPVKNALVML
                     KGENFGGFVDTTYSDRHGFYSLKVKRGWYSGMAVIDMEDYGKSKLEFWAFEIPAFENL
                     NIDARYDRLEIYGLNVFEIQGAYPGYTIYFRPMSLTRILSADMEVNIPEIAPPPEQLE
                     IRVEINGETVNVNHIQRVEEFAGEQMLYAFLIHTDPGKESAGKYDMIRISARDRGNGD
                     RGEAICFRERSRYE"
     gene            1243..4134
                     /locus_tag="PLZ75_07845"
     CDS             1243..4134
                     /locus_tag="PLZ75_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS72344.1"
                     /translation="MNNPFNVSEEKLKQLFRGYDAWIKSIPEKSAAPGEAKEEPKQLT
                     EQLLDPDTPATLSDADLYEKVYSYIKSIDVTVKTGAAKEGLRDSLHDIRRNLNYLITS
                     EDTPFMTAQNILEGEYRIEALPGAFWSPLFSARFPDKIPDWNKPAENFLKKLGIKLSP
                     SKTSVSEKYKLLSDAFAFLASLSEGHDFHTLSHLMFYATATAEGTELVNKIRGIRPDD
                     KILDIVSRFKERVRQEGLADELYKWQLLKKYRGRPDTSASDFNEEIRSIDLSNLVYHI
                     AAAVKNHIASTHPEEYRKCFIQLFDEDEDLNTRVQGFMNNVLSVYRKVEPVLSSHHDE
                     RTASVFLTYHDPGNYALYKDSFYRKYCSLTGVSPKPSGEKYAHYMELISEFRDNYILT
                     DKELISLVNDSLGPDCYPDESHLILAQNILYVMLDKDKSMANESELISVLKTAASRNY
                     AEAYFSILENLADKFGFTNDNPRLAFSVTRSDNKLSVTVNQRPVISTDSGNDNIFGSS
                     DIIWLLIPASRSDSLREHEHFIKLSGEDAYEKMPGETVAPSRAAFNIFSVIYDESILE
                     AWYEAVKSELDHGTKSPFRKSHNSAFLDAVIDPDYRELLFKKAYGPDIPVMNLNTLLY
                     GPPGTGKTYSTIDLAVRITSPEDYSPGNHQANRKVYEKLVRDGRIVFTTFHQSLSYED
                     FIEGIKPSADNSGNLTYSVEDGLFKRLAINAAFEYAGERHPEENKSTVIQKLLEQDIV
                     DTEDRKKYVLIIDEINRGNVSQIFGELITLIEEDKRYGNEEGLSAILPYSKSKFFVTP
                     NLYIIGTMNTADRSVEALDTALRRRFSFIEMTPQYDLDGMDNMISGYRISELLETLNQ
                     RIEKLVDRDHRIGHSYFLGLKNPSDLMSVFRNKIIPLLQEYFYGNYDKMGLVLGSGFI
                     EKIADESVRFASYQADENDFAHKQVCRINKRSLKEIAEFEKALAALMNK"
     gene            4144..5430
                     /locus_tag="PLZ75_07850"
     CDS             4144..5430
                     /locus_tag="PLZ75_07850"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72345.1"
                     /translation="MEASGSDKVFRVFEHSSYNTRNPGREGKILPEELFAALKDFAGE
                     RDLPWYSLTANGVRFHQYVGAIQIGKYCVEVLPKIDRGRQNEEPAQTVLINMLKQCGY
                     FSVNTPTESDLKLRSNFILEVYIKMFLDEVRDLMHRGLVKHYHKTEGNNNYLKGSLLF
                     NKHIHRNLIHAERFYSRHTIYDRDHFLNRILRKTLKVIARLPVSSEATGTALNLLSLF
                     PELGDIEVNDELFDKIRYHRKTEAYRKSVEVSRLLLLNYHPDLNHGRNNVLAIMFDMN
                     DLWETWFTKRLIIAARDYPDVSVRPQYRKVFWTSTESVIRQKPDIIIEIDSQPGCVID
                     TKWKIISGRPSEEDVRQMFAYNKLFNTRKSWLVYPGETASVTGRFYESENNGECSLAS
                     IMFHRDGRLCDAGIREFASELLSMQMILIICYGQFK"
     gene            complement(5570..5974)
                     /locus_tag="PLZ75_07855"
     CDS             complement(5570..5974)
                     /locus_tag="PLZ75_07855"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOS72346.1"
                     /translation="MKTIIVILERSENNYSAYIPDLDGCVATGETIDQVKEHIKEAVE
                     FHLDGMKEEGLDIPEALQNDYNFVYKIDVESLFEWFSGVLTKSGIAKLTGMNQSLLSQ
                     YANGIKKPSKKQSKKIEKAIHNFGEELLSIQF"
     gene            complement(6100..6285)
                     /locus_tag="PLZ75_07860"
     CDS             complement(6100..6285)
                     /locus_tag="PLZ75_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOS72347.1"
                     /translation="MKVSDLIEILKEDGWVLSRTKGSHRQFKHSNKKGVVTVSGKLSD
                     DVRKGTLGSILRQAGLK"
     gene            complement(6492..>7918)
                     /locus_tag="PLZ75_07865"
     CDS             complement(6492..>7918)
                     /locus_tag="PLZ75_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOS72348.1"
                     /translation="FSFVSVDKARKNLEAEMNDKNFAQVVKEADKTWEDLLSKIKVTG
                     GTEKQKRTFYSCLYRALQWPAIRSDIDGEYTDSRGNTVNKGFNYYTSNNYWDTWQSKM
                     VLMEMLESELTADVIRSDIERGKVGGFLSSGFHGDFSTSFITGSYLRGIRDYDIDSAY
                     YLALNNATKVAEGPRRGGRRYLSEYIDRGWIAENRVENPTTGTEEDEAKASVSKTIEY
                     AYSDYSVALLAKELGDMDNYHMLMQRSENYKNLFDTSTLFMRGRFDNGDWVTPFYPDY
                     PYYVYMYRESTGWQATFFAPQNPSGLIGLFPNKKIFEQKLDSLFIIPWGGYEADNLTG
                     FLGQYCAGNQPSQGIAYYYYFIGKQEKCQEKLDIIMEKYYDMGKEGLAYAGMDDEGGL
                     SSWYVLNAIGLFPFSPADPEYIITVPIFDRIVFDLDGIPFTISKINNGRKISSITYDG
                     RKIDDYFVSHDDLKKGKELKITTE"
BASE COUNT         2422 a         1687 c         1532 g         2277 t
ORIGIN      
        1 aaaattttaa tcggcataat cagatcagtt tatcaccctg ataattgctt caaaccattt
       61 cctgaaatat ccggataggg gaagcatgat ttattatcac acaaattaaa aatattgtaa
      121 ggaatagcca aattatgaat caggataatc ggcaataatc atatcaggat tataacgatg
      181 taggtaccag gcccaaagat aacactcctg atatgataag gatcctgcgg cccggaacag
      241 ttactccgct gaggctaatg aagcttctgt cacgactggt ttatctgtca ttcatacctt
      301 gacctttccc tgaaacagat tgcctctccc ctgtctccgt ttcccctgtc cctggctgat
      361 atccttatca tatcatattt gccggcagac tctttgccgg gatctgtatg aatcaaaaag
      421 gcatacagca tctgttcccc cgcaaattct tccactcttt gtatatggtt aacatttaca
      481 gtctcaccgt ttatttcaac ccggatttcc agctgttccg gcggcggagc tatttcaggg
      541 atatttacct ccatgtcagc agacaaaata cgtgtaaggc tcatcggtct gaaataaatt
      601 gtatatcccg ggtatgcacc ctgtatttca aaaacattaa gaccgtaaat ttcaagcctg
      661 tcatatctgg catcaatatt cagattttca aaagcaggta tttcaaatgc ccagaattcc
      721 agtttgcttt taccgtaatc ctccatatca atgacagcca taccggaata ccatcccctt
      781 tttaccttaa gggaatagaa accgtgccgg tctgaataag tggtatcaac gaatccgccg
      841 aaattttcac ctttcagcat taccagggca ttcttcacag gcctgtcgtc gaagtcggca
      901 acctttccgc agattgacac ggaatcattc tgacctgaag ccacaataat attaaaaaca
      961 agaacaagga acagtaatct tttcatgacg gtattatgtt atggaatgta aaaaaaatat
     1021 aagtattaag taaaaacctg atccgggtgc aatttctgag tttatcgaaa tttatgcaca
     1081 agttcacaaa aatttatgag atagaacagg gattatatat aaaccggcaa tcgaaaagct
     1141 ttccaaccgg cttacatctt tatttccggc caaaaaatcc atacgcaccc catattttac
     1201 tattttagta gacttatatg gttccctcta aatcaaagta caatgaacaa tccgtttaac
     1261 gtatcagagg agaagctcaa acagctcttc cggggatatg atgcatggat taaatctatt
     1321 ccggaaaaat ccgcagcccc cggagaagca aaagaagaac cgaaacagct gacagagcag
     1381 ttacttgatc cggatacgcc tgcaaccctg tctgatgctg atctgtatga aaaagtatac
     1441 agctatatta aaagcattga cgtaacggtt aaaaccggag ccgcaaaaga aggattacgg
     1501 gattccttac atgatatcag gcggaattta aactatctca ttacatcaga agacacgccc
     1561 ttcatgacag ctcaaaatat tcttgaaggc gaatacagaa ttgaggcgct tcccggagca
     1621 ttctggagtc cgttatttag tgcccgtttc cctgataaaa taccggactg gaataaaccg
     1681 gcagaaaatt ttctaaaaaa gttaggaata aagctctctc cctcaaaaac atcagtttca
     1741 gagaaatata aactcctgtc ggatgcattc gccttccttg catccttatc tgaaggtcac
     1801 gattttcata ccctcagcca tctgatgttt tatgccacag caacagctga aggtacagag
     1861 cttgtaaata aaatccgggg tatcaggccg gatgataaaa tcctggatat tgtctcacgg
     1921 tttaaggaga gggtgagaca agaaggcctg gctgatgaac tgtataagtg gcagctgcta
     1981 aagaaatacc ggggaaggcc tgatacttcg gcctctgatt tcaacgagga aataagaagt
     2041 attgacttaa gcaatcttgt ttatcatatt gcagcagcgg ttaagaacca tattgcttcc
     2101 acccatcccg aagaatacag aaaatgcttt attcagctct tcgatgaaga tgaggatctg
     2161 aatacccgcg ttcaaggatt catgaataat gtcctttcag tttatcggaa agttgaacct
     2221 gtactgagca gtcatcatga cgaacggact gcctctgtgt ttctcactta tcatgatccc
     2281 gggaattatg ccctgtacaa ggattccttc tacagaaaat actgcagcct tacaggtgtc
     2341 agtccgaaac cctccggaga aaaatacgcc cattacatgg aacttatctc tgaattcaga
     2401 gacaattata ttttaaccga caaggaatta atttcactgg taaatgattc cctcggacct
     2461 gactgctatc ctgatgaatc ccatttaatc ctggcccaaa acattttata cgtgatgctc
     2521 gacaaggaca agagcatggc caatgaatca gagcttatca gcgtacttaa aaccgccgct
     2581 tcaagaaact atgcggaagc atatttcagt attctggaaa accttgctga taagttcgga
     2641 tttacaaatg acaacccaag actcgccttt tcagttaccc ggtctgacaa taaattaagt
     2701 gtaacagtaa atcaaagacc tgtaatcagt acagattcag gaaatgacaa catattcggg
     2761 tcttcagata ttatctggct tcttataccg gcttcccggt cggattccct cagggaacat
     2821 gaacacttta tcaaactatc cggcgaagac gcgtatgaaa agatgccagg ggagactgta
     2881 gctccctcac gggcggcttt caatatattt tccgtcatat atgacgaaag catattggag
     2941 gcctggtatg aagctgtgaa aagtgaactt gaccatggca cgaaatcacc tttcaggaaa
     3001 tcccataatt ctgcatttct ggatgcggta attgatcctg actacagaga attattattt
     3061 aaaaaagcat atggaccaga tatcccggtt atgaacctca acaccctact ctacggcccc
     3121 cccggcacgg gcaaaaccta ttcaaccatt gacctggctg tacggatcac ctcaccggaa
     3181 gattattccc ccggcaatca ccaggccaac aggaaagtat atgaaaaact ggttcgtgat
     3241 ggaaggatcg ttttcaccac attccaccag agcctgagct acgaggattt tatcgaagga
     3301 atcaagccat cagctgacaa cagcggcaat ctcacctata gtgttgaaga cggtctgttt
     3361 aaacgccttg caataaacgc tgcatttgag tacgccgggg aaaggcatcc tgaggaaaac
     3421 aaatccactg tgatacaaaa attactggaa caagatattg ttgacacgga ggataggaaa
     3481 aagtatgttc tgataataga tgaaattaac agaggcaatg tatctcaaat ctttggcgaa
     3541 cttattaccc tgattgagga agacaaaaga tacgggaatg aagaaggcct ttccgcaata
     3601 cttccgtatt caaaatcgaa atttttcgta acgcctaatc tgtatattat cggaacaatg
     3661 aatactgccg acaggagtgt ggaagccctt gatacagccc tgcgaaggag attctcattt
     3721 attgaaatga ctccgcagta cgatctggac ggaatggata atatgatatc cggataccgc
     3781 atttcagagc tgcttgaaac attaaaccag cggattgaaa agcttgtcga cagggatcac
     3841 aggataggtc attcgtattt cctgggatta aaaaatcctt cagacctgat gagtgtattc
     3901 aggaataaaa taatccccct gctgcaggaa tatttctatg gaaattatga taagatggga
     3961 ctggtactgg gtagcggatt cattgaaaag atagctgatg agagcgtgag gtttgccagc
     4021 tatcaggctg atgagaatga ttttgcccac aagcaggtat gcaggataaa taagagatcg
     4081 ctaaaggaaa tagcagagtt tgaaaaagcc ctggcagcat tgatgaacaa gtagagcatt
     4141 gtattggaag caagcggatc agataaggta ttccgggttt ttgagcacag ctcatacaac
     4201 acaaggaatc ccggcaggga aggcaaaata cttccggaag aactgtttgc tgctctgaaa
     4261 gattttgccg gcgaaaggga cctgccctgg tacagtctca ccgcaaacgg cgttagattt
     4321 catcagtatg tgggtgccat ccagataggg aagtattgtg tggaagtcct gccaaagatt
     4381 gacaggggcc ggcagaatga agaacctgct cagacggttc ttataaatat gcttaagcaa
     4441 tgtggttatt tttcagtcaa cacccccacc gagtcagatc tgaagctcag gagcaacttc
     4501 atccttgaag tatacatcaa aatgtttctc gatgaagtca gggatctgat gcaccgcggt
     4561 ctggtaaaac attaccacaa aacagaaggt aacaacaatt acctgaaagg aagcctttta
     4621 tttaacaagc atattcacag aaaccttata catgctgaaa gattctattc gaggcacacc
     4681 atttacgatc gggaccattt tttaaaccgt atcctcagga aaacattaaa agtaatagca
     4741 cggcttccgg tcagcagtga ggcaacggga actgccctca acctgctttc ccttttccct
     4801 gaactcgggg atattgaagt gaatgatgag ctgtttgaca aaatccgcta tcacaggaaa
     4861 acagaagcat acagaaaatc agtcgaggta tccaggttat tattgctgaa ctatcatccg
     4921 gatctgaatc acggacgaaa taatgtactg gcgataatgt ttgacatgaa tgacttatgg
     4981 gagacctggt ttacaaaaag acttattatt gccgccagag attatcccga tgtatcagtt
     5041 aggcctcaat accgaaaggt attctggaca agcacagaaa gtgtcattcg ccagaaaccc
     5101 gatataataa tcgagattga ctctcagccg ggctgtgtaa ttgacacaaa atggaagatt
     5161 atctccggca ggccgtccga agaagatgta aggcagatgt ttgcctacaa caaactgttc
     5221 aataccagga aatcgtggct tgtttatccc ggcgaaaccg catcggtaac aggcaggttc
     5281 tatgaatctg aaaacaacgg agaatgcagt ctcgcgtcaa taatgtttca ccgcgacggc
     5341 aggctctgcg atgccgggat aagggaattt gcttcggagc tcctgagtat gcagatgatt
     5401 ttgattatct gttatggtca attcaaatag tcaatacagc ttataaaaca taccgaccat
     5461 ctcaggtgtt aaagctattt tgtcttgatg gaattactac gttcttttga tgttttaact
     5521 atttgaaaag ctgccacgct ttggcagctt ttcaaatagt tatgaattat taaaactgaa
     5581 tactaagaag ttcttctcca aaattatgta tcgctttttc aatctttttt gattgctttt
     5641 tgcttggttt ctttatgcca tttgcgtatt ggcttaataa actttggttc attccagtga
     5701 gttttgcaat tccagatttg gttagtacac ctgaaaacca ctcaaataat gattcgacat
     5761 caattttata aacaaaatta taatcatttt gcagtgcttc aggaatatct aaaccttctt
     5821 ccttcattcc gtcaagatga aattctacag cttctttgat atgttcctta acctgatcta
     5881 ttgtttctcc agttgcaaca catccgtcca agtcgggaat ataagctgaa taattattct
     5941 ctgacctttc caagatgact ataatggttt tcatattttc gtagttggtt tgaataattt
     6001 gttttaataa tttccttaag ttccagtgtg tcttagaaat cttggataca tatattgaag
     6061 cttcagattt aattttaact cacactaaga cgagccgaac tatttgagtc cggcttgtct
     6121 taaaatgctg cctaaagtac cttttctaac atcatcagat agtttaccag atacagtaac
     6181 aacacctttt ttgttagaat gtttaaattg ccgatgactt cctttagttc ttgaaagcac
     6241 ccaaccgtcc tcctttagga tttcaatcaa atctgatact ttcataatat tccgttttaa
     6301 attttcaaag aacgcaaggt ctcaattcaa aaactcacca caaaagtaat gaaaacatta
     6361 cttatttcca gacattttta gtgaaaaagt aataatatta ttactgaata ttctgattcg
     6421 tcatgaaaaa aattattata aataagtcta taatgctagt attatgctgt ttgcgtctta
     6481 cgtctaaatt gttattcggt tgttattttc aattcttttc cctttttcaa atcatcatgc
     6541 gacacaaaat aatcatctat ctttcgtcca tcatatgtga tgctgctgat ctttcttccg
     6601 ttatttattt tgctgatggt aaatggaatt ccatccagat caaaaacaat tctatcaaaa
     6661 ataggaactg tgataatgta ttccgggtct gcaggtgaaa atggaaataa tccgatagca
     6721 ttcagcacat accatgacga caatccgcct tcatcgtcca ttccagcgta tgccagtcct
     6781 tctttgccca tgtcgtaata tttttccata ataatgtcca gtttttcctg acatttctct
     6841 tgtttgccga tgaaatagta atagtatgct ataccttgtg aaggctgatt acctgcacag
     6901 tactgaccaa gaaaacccgt taaattatca gcttcgtagc ctccccaggg aatgataaac
     6961 agtgaatcaa gcttttgttc gaaaatcttt ttgtttggga aaagccctat aagccccgat
     7021 ggattttgcg gtgcaaaaaa cgttgcttgc cacccggttg attcgcggta catataaaca
     7081 tagtaagggt aatcaggata gaaaggtgta acccagtcgc cattatcaaa tcgaccgcgc
     7141 ataaaaagtg ttgatgtgtc gaacagattt ttataatttt ctgagcgttg catcaacatg
     7201 tgataattat ccatatcacc taactctttg gccagaagtg caactgaata atcactgtaa
     7261 gcatattcta tcgttttgga cactgatgct tttgcctcgt cttcttcggt cccggtagta
     7321 ggattttcaa cacggttttc ggcaatccat cctctgtcaa tgtattcact gagataacgc
     7381 cttccgcctc ttcttggtcc ttctgccact ttagtggcgt tgttcaatgc taaatagtaa
     7441 gcactgtcaa tatcataatc acgaataccg cgaagataac tgccggtaat aaatgatgtg
     7501 gaaaagtccc catgaaaacc acttgacaag aagcctccta ctttacccct ttcaatatct
     7561 gagcgaataa catcagctgt taattcggat tccagcatct ccatcaacac cattttagat
     7621 tgccaggtgt cccagtaatt attactggta taataattaa accctttatt gactgtattg
     7681 ccacgtgaat cagtgtattc accatcaata tcgcttcgaa tcgccggcca ctgtaacgct
     7741 ctatacaggc acgagtagaa tgtccgtttt tgtttttcag ttccgccggt tacctttatt
     7801 ttcgataaaa ggtcttccca tgtcttatca gcctctttga ctacttgtgc aaaatttttg
     7861 tcattcattt ccgcttccag gtttttcctg gccttgtcaa cgcttacaaa tgaaaaac
//
LOCUS       DAOSPX010000136         7881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8671_length_7881_cov_3.948633, whole genome shotgun sequence.
ACCESSION   DAOSPX010000136 DAOSPX010000000
VERSION     DAOSPX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8671_length_7881_cov_3.948633"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="PLZ75_07870"
     CDS             complement(<1..1427)
                     /locus_tag="PLZ75_07870"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOS72349.1"
                     /translation="MILSTGCSDIRSDDDFDPVELVSPLMGTDSEFRLSNGNTYPAIA
                     LPWGMNFWTPQTRRMGDGWAYTYNDVKIMGIKQTHQPSPWINDYAAFSLMPVTGKLRI
                     REDERASWFSHKAEIAKPYYYSVYLADYDVTAEVTPTERGAIFRFTFPENDSSYILVD
                     GFNGGSMVKIIPSERKVVGYCRNNHGGVPENFHNYFVAVFDKDFSLAHTWNGDVLTPG
                     ILENEGRHTGAVLGFKTARGEKISVKVASSFISPEQAELNLRRETGNKSFDELVRKGK
                     KIWNDELKKIQVEGGTSDQQRTFYSCLYRMLLFPRAFHELDENNAVVHYSPYNGEVLP
                     GYMYTDNGFWDTFRAVFPFFTLMYPELNSRIMEGLANTYRESGWLPEWASPGHRDCMI
                     GSNSASLIADSYLKGIRGYDIDILYEAILKNTLGAGPVSSVGRLGAEYYNRLGYVPYD
                     VGINENTARTLEYAYADFCIWKLAR"
     gene            complement(1519..2547)
                     /locus_tag="PLZ75_07875"
     CDS             complement(1519..2547)
                     /locus_tag="PLZ75_07875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOS72350.1"
                     /translation="MHLLVIRTSAMGDVALTVPVIGSLRVQHPEIRVTLLTRPLFRPF
                     FSSLPSLDIRDARFDGIHKGFTGLIRLYRDCTRDGDYDYIIDLHDVLRTKILRWLFRL
                     SGKKSFVIDKGRREKRKLIRGKNKSALRHTVERYLDVFSRAGINIDPMKWKGILPAPG
                     AIEKIGGIIDNSAVMNIGIAPYARHPLKVWPEEYVRQLAEMISGKQDVKFWLFGGRDE
                     YGRLSVLQSAIPGSVLAAEDLTLDEQLALMSRLDLMISMDSANMHMASLAGTKVISVW
                     GATDPLAGFGAWNQPVEFSMSIPVDELTCRPCTVYGKGKCRRGDFACMRWLLPGMIFT
                     RLIQLELL"
     gene            complement(2549..2920)
                     /locus_tag="PLZ75_07880"
     CDS             complement(2549..2920)
                     /locus_tag="PLZ75_07880"
                     /inference="COORDINATES: protein motif:HMM:NF039364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HOS72351.1"
                     /translation="MKIEVSNGEIIDKLTILRIKSERIRDKDKLMNIMKEYDELKNQA
                     SSILSQDDPLYQALYDINNELWDIEDNIRELERRKDFGREFIETARSVYFRNDKRAEI
                     KREINIRTSSGYIEEKSFGKY"
     gene            complement(3074..3733)
                     /locus_tag="PLZ75_07885"
     CDS             complement(3074..3733)
                     /locus_tag="PLZ75_07885"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOS72352.1"
                     /translation="MDQDTTRGKNSFSRIIREFEDRRIDILIGTQMISKGLDFENLTV
                     VGILNADSMLNFPDFRAHERSFQLMEQVSGRAGRREKQGRVVIQTADPANRIIRLVLR
                     HDYEGIYRIQAEERRTFNYPPFCRMIRIVLKHRDKMQLNNFSGRLADELRNYPGYHVL
                     GPEFPVISQIQLYHIKTILIKIEKQRSLSGAKEKIAGSIGRIRKMKGSGGLKISVDVD
                     P"
     gene            complement(3776..5359)
                     /gene="priA"
                     /locus_tag="PLZ75_07890"
     CDS             complement(3776..5359)
                     /gene="priA"
                     /locus_tag="PLZ75_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOS72353.1"
                     /translation="MCHSYADIILPLAIKGRLTYSIPVNLQDIAKPGVQAMVQLGSSK
                     IYTGIIGKIHHEKPDLDNIRPIVRIVDPLPVINEKQLIFWKWMSDYYMCSEGEVIKAA
                     IPGIFFPEGDISMHVSEKYTPRLVTFIRLAGDYSEKGLNEIRDKLRKAPKQLDLINTY
                     LHLSGLNTKTEPRPIKKTLLLRKAGYSPAALQGLVKKGILQCISLEVSRLEDKVYETE
                     PLNRLSITQHSVLKSVQDQFLHRDVLLLHGITSSGKTEIYIHLIEEQLKKGKQVLYLL
                     PEIALTTQIIKRLRKHFGNITGIYHSRFSDSERAEIWKKVAENDIEKGYRLILGVRSS
                     IFLPFNNLGLVIVDEEHDTSYKQNEPAPRYHARDSAIMLAGFHEAKTILGSATPSVES
                     YSNAISRKYGFAELTERYGKVRLPEIILADTREAYRKKLMVSHFTPELLNAMDQALAR
                     QEQIILFRNRRGFAPWVECSECGWIQLCRQCAVSMTYHKAEGNMLCHYCGDRRPVPSK
                     CGNCGSTGLATRGFGTEKI"
     gene            5922..6680
                     /locus_tag="PLZ75_07895"
     CDS             5922..6680
                     /locus_tag="PLZ75_07895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS72354.1"
                     /translation="MRGKFKLIFTRHSDGKPAKLSCLLMKKADHLVTLNSTMAENIPY
                     PNTLIKHGVNIDIFKIHKKQKIANIHQKNLISIIGRIRPPKGQIVVLRALTAILRNNS
                     DWGLVMIGKIDDKKYAEEIMSMASENGIASQVYIMPESNKIIDYYFASNIIVIASFTE
                     GFSLVCLEAMACGLITVATESVGIHSKVINHGENGFLFPKNDHEYLRKILTDIISGKI
                     NLDPDKIRQTVIDKWSLERSVKDLLKLYEISQVN"
     gene            complement(6890..>7881)
                     /locus_tag="PLZ75_07900"
     CDS             complement(6890..>7881)
                     /locus_tag="PLZ75_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS72355.1"
                     /translation="LLQIIGDIIDLSRLESSQIRISNEEVPVGRVVKEVIEEAKQVIR
                     RNEKNINLNVITRLDEKTDRILSDRVWLKRVLNHLMDNAIKFTLDGTVELSYYTQNGD
                     LFFRVKDTGIGINKENLARIFEEFQQEIDGHHRPFEGLGIGLTLVREVIHRMGGKIIV
                     QSEKGVGSVFTFSIPYIRTGGCEPASADTEKLQPEETDIDWSSNKCLLVDDNIDVLTY
                     LKTILVDTGINVVTASSGASAIETIRNTPDLDLILLDLQMPEINGLDVTREIRKISSS
                     LPIIAQTAYIFEEDKDSILRAGCDACLIKPILKENLITVMSSFIRTKPSSDRT"
BASE COUNT         2102 a         1843 c         1554 g         2382 t
ORIGIN      
        1 ttcctggcca gcttccagat acagaaatcg gcataggcat attcaagggt acgtgctgtg
       61 ttttcattta tgccaacatc atagggcaca taaccaagcc tgttgtagta ttccgccccg
      121 aggcgcccca ccgacgaaac aggtccggcg ccaagggtat ttttcagtat tgcttcatac
      181 agaatatcaa tatcatatcc ccgtatcccc ttcaggtatg aatcagcaat cagggaggcg
      241 gagtttgaac ctatcatgca gtcacgatga cccgggctgg cccattccgg gagccatccg
      301 ctctccctgt aggtattggc aagtccttcc attatcctgc tgttcagttc aggatacata
      361 agcgtgaaaa agggaaagac tgccctgaaa gtatcccaga atccgttatc ggtatacatg
      421 tatccgggaa gcacttcccc gttatacggg ctataatgaa caacagcgtt gttttcatcc
      481 agttcgtgga aagcccgcgg aaagagaagc attctgtaaa gacaggaata gaaggtcctc
      541 tgctggtcgg aggttccacc ttccacctgt atctttttca gttcatcatt ccatattttc
      601 ttacccttcc tcacgagctc gtcaaagctt ttgttgccgg tttcacgcct gaggttgagc
      661 tcggcctgct caggactgat aaaggaagaa gcaactttca cactgatctt ttcaccacgg
      721 gcggttttga agcccagtac agccccggta tgcctgcctt cattctccag aatgccggga
      781 gtaagcacgt cgccgttcca ggtgtgtgcc agggagaaat ctttatcaaa aacagcaaca
      841 aaatagttat ggaaattttc gggtaccccg ccgtggttgt tcctgcaata tcccaccact
      901 ttccgctctg atggaatgat cttcaccatt gatcctccgt tgaaaccatc aacaagaata
      961 tatgatgagt cgttttcagg aaaagtgaac ctgaatattg ctcctctttc agtcggtgtc
     1021 acttcagcag taacatcata atcagcaagg taaacactgt aatagtatgg tttggcaatt
     1081 tcggccttgt gggagaacca tgatgccctt tcatcctccc ttatcctgag ttttcccgta
     1141 accggcatca gggagaatgc cgcataatca ttgatccagg gactgggctg atgtgtctgc
     1201 tttataccca ttatctttac gtcattatag gtatatgccc agccgtcgcc catcctgcgg
     1261 gtctgcggcg tccagaaatt cattccccag ggaagggcta tcgccgggta agtattcccg
     1321 tttgaaagcc tgaattcgga gtcagtgccc ataaggggac tgacaagctc aacaggatca
     1381 aaatcatcat cggatctgat atctgaacat cccgttgaca ggatcatcgg gcacagcata
     1441 ataaatgccg caactgaaac gaaaccttca gggcggagca ctttccggaa aagtctgata
     1501 atattcataa tcatggtttt atagtaattc aagttggata agccttgtaa atatcattcc
     1561 tggaagaagc catctcatgc atgcaaagtc tcctcttctg cattttcctt ttccgtaaac
     1621 agtacatggc ctgcatgtaa gttcatcaac aggaatactc atggagaatt ccacgggctg
     1681 gttccatgcg ccaaaacccg cgaggggatc cgttgccccc catactgaaa tcacttttgt
     1741 tcccgcaagc gaagccatat gcatatttgc agaatccatg ctgatcatca ggtcgagtct
     1801 gctcataagt gccagctgtt catcaagggt cagatcttca gcagcaagaa ctgatcccgg
     1861 aatagctgac tgaagaaccg aaagccttcc gtattcatcc ctgccgccaa aaagccagaa
     1921 ttttacatcc tgcttcccgg agatcatttc cgcgagttgc ctgacatatt cctcaggcca
     1981 tactttcaga ggatgcctgg catatggtgc gatccctata ttcatcacag cgctgttgtc
     2041 aataatgccg ccaatctttt caattgctcc cggtgcaggt aaaatgcctt tccatttcat
     2101 cggatcgata ttaatacctg ccctggaaaa aacatccaga tatctttcaa ctgtgtgtct
     2161 gagcgcggat ttgtttttcc cccttatgag ttttcttttt tcccttctgc ccttgtcaat
     2221 cacaaaggat tttttcccgg aaagcctgaa cagccatctc agaatttttg ttctcagtac
     2281 gtcatgaaga tcaataatat aatcataatc cccgtctctt gtacagtccc ggtaaagtct
     2341 tatcagtccg gtgaatcctt tatggattcc atcgaagcgg gcatcacgga tatcaaggga
     2401 aggcagcgaa ctgaagaaag gcctgaacag gggtcttgtc agcagggtca cccttatttc
     2461 gggatgctgc acccggagag aaccgattac cggcactgtc agggcaacat ctcccatagc
     2521 cgatgtcctg ataacaagaa gatgcatttc aatactttcc gaaagatttt tcttctatat
     2581 aacctgaaga tgtcctgata tttatttccc tttttatttc tgccctttta tcgttcctga
     2641 aataaactga acgggccgtt tcaataaact ccctgccaaa atcctttctg cgttccagtt
     2701 cacggatatt gtcctcaata tcccacaatt cattgttgat gtcgtagagg gcctggtaaa
     2761 gaggatcatc ctggctcagt atggaggatg cctgattctt cagctcatca tattccttca
     2821 tgatattcat gagcttgtct ttatccctga tccgttcgct ctttatcctg aggatggtaa
     2881 gcttatctat aatctctccg tttgatactt ctattttcat actcaattat ttcagttatt
     2941 tcagttattt caattacttc agcagcctca ttgtttcaac tgttccgctt gcccgggtta
     3001 gtgtggctgc ttcagttacc tgctggctta ttctatcaaa aataacaaat tttttccgca
     3061 tccgtaataa cagctaggga tctacatcaa ctgatatttt caaaccgccg gaacccttca
     3121 ttttccggat cctgccgatg gatccggcta tcttttcctt agccccggaa agagatctct
     3181 gtttttcaat cttaatcagg atcgttttga tatggtaaag ctgtatctgt gatataaccg
     3241 ggaattcagg accaagcaca tggtaaccgg gataatttct gagctcatct gccagccggc
     3301 cggaaaagtt gttaagctgc attttgtcgc ggtgtttcag cactatcctg atcatacggc
     3361 agaaaggcgg atagttgaat gtcctccgct cttcagcctg tatccggtat attccttcat
     3421 agtcatgacg cagaaccagc ctgataatcc ggttcgcggg atcagccgtc tgtattacaa
     3481 cccttccctg tttctctctt cgtcctgccc tgccactaac ctgttccatc agctgaaagc
     3541 tccgttcatg tgccctgaaa tcggggaaat tcagcatact gtcggcattc aggatcccga
     3601 caacagtaag gttttcaaaa tcaagaccct tagatatcat ctgtgttccg atcagtatat
     3661 ctatgcgtct gtcctcaaat tccctgatta ttctgctgaa tgaattcttg ccccttgttg
     3721 tatcctgatc cattctggcc acgcgggctg agggaaaaac aatctttatt tcatcctata
     3781 tcttttcggt tccgaatccc cttgtcgcaa gtccggtgct tccacagttc ccgcattttg
     3841 acggcaccgg ccttctgtcc ccgcagtaat ggcaaagcat gttcccttca gccttgtgat
     3901 atgtcatgct cacggcgcat tgcctgcata actgtatcca gccgcattca gaacattcca
     3961 cccagggcgc gaatcccctc ctgttcctga acaggataat ctgttcctgc ctggccaggg
     4021 cctgatccat ggcattaaga agctcggggg tgaaatgaga aaccatcagt tttttcctgt
     4081 atgcctctct tgtgtcggca agaattatct caggcagcct gactttgccg tacctttccg
     4141 ttaattctgc aaatccgtat ttacggctga tggcattgct gtagctttcc accgacgggg
     4201 tggctgatcc cagaatggtt ttcgcttcat gaaatcctgc cagcataatg gctgaatcac
     4261 gcgcatgata gcgcggagca ggctcattct gcttatatga agtatcatgc tcctcatcaa
     4321 ctattaccag tccgagatta ttgaacggaa ggaaaattga tgatctcact ccgagtataa
     4381 gcctgtatcc tttctcaatg tcattttctg caactttctt ccagatttcc gccctttcag
     4441 agtctgaaaa ccttgaatga taaattccgg taatattccc gaaatgcttt ctcaacctct
     4501 taattatctg tgtcgtaagt gctatttcag gcaacagata taaaacctgt ttcccttttt
     4561 taagctgttc ttcaataaga tgaatatata tttcagtctt accgcttgaa gtaatgccat
     4621 gcaaaagaag cacatcccgg tgcaggaact gatcctgaac agatttcaga actgaatgtt
     4681 gagtgatgct gagcctgttc agaggttctg tctcataaac tttatcttcc agtctgctga
     4741 cttcaagaga tatgcactga agaatacctt tctttaccaa tccttgcagt gcggctgggg
     4801 aatatccggc ttttctcagc aataatgttt tttttatagg tctcggctca gtttttgtat
     4861 ttaacccaga gagatgtaag tatgtattga tcagatccaa ctgcttagga gcttttctca
     4921 gcttatcacg aatctcattc aaaccttttt cagaatagtc cccggcaagc ctgataaaag
     4981 ttaccaaccg gggcgtatac ttttcgctga catgcattga tatatcacct tcagggaaga
     5041 aaattccggg aatggccgct tttatgactt ctccttcgga acacatgtaa taatccgaca
     5101 tccatttcca gaaaatcaac tgtttttcat taatgaccgg gagtggatca acaattctaa
     5161 ctattggtct gatattgtca agatccggtt tttcgtgatg tatcttccct ataatacctg
     5221 tgtaaatctt actgctaccc aactggacca ttgcttgcac accgggcttt gcaatatcct
     5281 gaagattaac cggtatactg taagtcaatc ttccttttat tgcaagagga aggataatat
     5341 ccgcatacga atgacacatt agcgcaaaat tgtgtacaat gtaagaagaa aaaagaagag
     5401 atctgtctgc ggatcaactc aacgtgacaa atgataacat aaccgcgaca cgattaacat
     5461 ttatttccaa aattatttaa taacaccctg cattaaacct gattaacatt attcatgaat
     5521 tgatttatga atcctttgga tatatacaat tatcaatata taataaacag ctgatatttg
     5581 gtttgtgaat gaaaacgcta attacctgca ttttaaacac tagtcattta tacaaatcag
     5641 taagttcgtt tttaactatt tctgtaatat atttgtaaac tgaatccttc taaatgatca
     5701 ttcatttcca ttttcactcc agggctaccg ggattacaag gactgttgaa agtattgtgc
     5761 caattctcaa taagtattcc ggttcatttg tgttcggtta cggtataaaa gcaccaaaaa
     5821 taagttggat ttctttactt cgactcatct atagtgacag gaaacttgtt atccatacac
     5881 accggaatat tgaaattctt tttgcattgt ttctcagaaa aatgagagga aaatttaaac
     5941 tcatttttac cagacactct gatggaaaac ctgcaaaact aagctgtctg ctgatgaaga
     6001 aagctgacca tctggttact ctaaactcaa ctatggcaga aaatatacct tatccaaaca
     6061 ccctgatcaa acatggagtc aacattgata tcttcaaaat tcacaaaaaa cagaaaattg
     6121 ccaatatcca tcagaaaaat ctgataagca taattggacg gatacgccca cctaaaggtc
     6181 agattgtggt tctaagagcc ttgacagcca ttctcaggaa taattctgat tggggcttgg
     6241 ttatgatagg aaagatcgat gataaaaaat atgcagaaga aataatgtca atggcatctg
     6301 aaaacgggat agcctcacag gtttatataa tgcctgaaag taataagatt attgattatt
     6361 attttgcaag caatataatt gtaattgcct catttacaga agggttttcc ctggtctgtc
     6421 ttgaagcgat ggcatgcgga cttataacgg tagctacaga atcagtcgga attcattcaa
     6481 aagttataaa ccatggagaa aacggattct tatttccaaa aaacgatcat gaatacctgc
     6541 gtaaaatcct cactgatata atatccggga aaattaatct tgatcctgat aagatacgac
     6601 agacagtaat agacaaatgg agtctggaaa gatcagttaa ggatttattg aagttgtatg
     6661 agatcagtca ggttaactaa atatcagatt ctttcctttt actttaatgc tttgtttcag
     6721 ttctaagccg gatctgcatt aatcgggatt tcaatatctt gtttttcttt ccctgaattt
     6781 tcatcagtca cactgattca agtccttatc atttcttgtg cattgaacgg gaattgcatt
     6841 gtatttggca gttctgaagt acttttaatt tccatttatt tgatggatat tatgtacggt
     6901 ctgatgatgg cttagtcctt atgaatgaag acattaccgt aatgaggttt tccttcagga
     6961 tgggtttgat gaggcaggca tcgcatccgg cccggagaat actgtctttg tcttcttcaa
     7021 aaatatatgc cgtctgggca ataatcggaa gactgctgct gattttcctg atttccctgg
     7081 taacatcaag tccgtttatt tcaggcatct gcagatcgag caggatcagg tcaagatcgg
     7141 gagtgttccg gatggtttca attgccgagg ctcccgaact ggccgtaaca acatttattc
     7201 ccgtatctac cagtattgtt ttaagataag taagtacatc gatgttatca tccaccagaa
     7261 ggcacttgtt ggagctccag tctatatcag tctcctccgg ctgcagtttt tccgtatcgg
     7321 ctgatgcagg ttcacaccct ccggtacgga tatatggtat ggaaaatgtg aaaaccgatc
     7381 cgactccctt ttctgactgg acaataattt ttccacccat tctgtggatc acttccctga
     7441 ccagggtcag tcctattccc agtccttcaa agggacggtg atgaccgtct atttcctgct
     7501 ggaattcctc aaagatgcgg gccaggttct ccttgttgat tcctataccg gtatccttta
     7561 ccctgaaaaa cagatcaccg ttctgagtgt agtacgacag ttcgacagta ccatcgagag
     7621 taaattttat ggcattgtcc atcagatgat taagaacccg cttaagccat accctgtctg
     7681 acagtatcct gtcggttttc tcatcgagcc gggtaataac attcagattt atattcttct
     7741 cattccttct gataacctgt ttcgcttctt caatcacttc cttcacaacc ctgccgacag
     7801 gcacctcctc atttgagatc ctgatctgtg agctttcaag tcttgaaaga tcaattatat
     7861 caccgattat ctgaagtagc t
//
LOCUS       DAOSPX010000137         7815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8774_length_7815_cov_4.804253, whole genome shotgun sequence.
ACCESSION   DAOSPX010000137 DAOSPX010000000
VERSION     DAOSPX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8774_length_7815_cov_4.804253"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..964
                     /locus_tag="PLZ75_07905"
     CDS             122..964
                     /locus_tag="PLZ75_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOS72356.1"
                     /translation="MKWQGRERSDNVEDRRGMGTGGRMAIGGGIGTVLIVLIVLLLGG
                     DPSALLDDPGTMIRTEQYTPSAEEEELAYFVSVVLKDTETVWDEIFRKSGRTYRQPKL
                     VLFSGQVESACGYASASSGPFYCPGDEKVYIDLNFFSELQNRLGAHGDFAVAYVIAHE
                     IGHHVQNLLGILDQVNSQRAGLNQVQSNKLLVRLELQADFLSGVWAHYADRMLNTLEK
                     GDAEEAMNAAASVGDDVLQKKYQGRVYPDSFTHGTAAQRKEWFRKGFQSGDLRQGDTF
                     SADL"
     gene            complement(1015..1689)
                     /locus_tag="PLZ75_07910"
     CDS             complement(1015..1689)
                     /locus_tag="PLZ75_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOS72357.1"
                     /translation="MKKVRNIFLVLAGASLIISGCASMNKAQKGAAIGTAAGGALGAV
                     IGKVSGNTALGAVVGAAVGGTTGAIIGNKMDKQAEEIKNKVPDAKVERVGEGIVVEFS
                     SNILFAFDKSELSAEAKSNLDRLVTVLEAYPDTDIELQGHTDNKGSKAYNQTLSENRA
                     SAVSGYLAQKGIGSERMTIKGFGLTMPKYDNATEEGRAQNRRVEFLITANEKMIADAD
                     REAGNR"
     gene            2034..2498
                     /gene="lysM"
                     /locus_tag="PLZ75_07915"
     CDS             2034..2498
                     /gene="lysM"
                     /locus_tag="PLZ75_07915"
                     /inference="COORDINATES: protein motif:HMM:NF008399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HOS72358.1"
                     /translation="MGLISFIKNAGEKLFKSGEKGEQEKAELIRTHIGRFGFDTSRIQ
                     VTVDKDRVILSGTVDTTDNKNKVIVTAGNVDGIAAVEDRIVVTEPVRSAPPPPEKQFY
                     TVKKGDYLSKIAKEVYGNANKYHIIFEANRPMLKDPDLIYPGQVLVIPPLEE"
     gene            2685..4310
                     /gene="pruA"
                     /locus_tag="PLZ75_07920"
     CDS             2685..4310
                     /gene="pruA"
                     /locus_tag="PLZ75_07920"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOS72359.1"
                     /translation="MSNAVFNFEQPRNEPVLSYLPDSDERKFLEEELNLQSNNVIDIP
                     LIIGGKEIRTGKTGRIVKPGDHGHLLANYHMATEKEVRLAINAALDAREQWLELEWIE
                     RAAIMSKAAELISKKYRYRLNAATMLGQGKNAYQAEIDSACELIDFLRFNNYFAYLIF
                     TGQPLSEDHSMNRIEYRPLEGFVYAVTPFNFTAIASNLNNSVALMGNTTVWKPASTAV
                     LSNYYLMEVMKEAGLPDGVINFVPGSGPLISSVVFGHREMAGIHFTGSGSTFTTLWKQ
                     ISDNLPLYRSFPRIVGETGGKNFIVAHSSADPLALATAVVRGAFEYQGQKCSAASRAY
                     IPRSIWEETSEYIYRMVSDIRIGDVTDLNNFVNAVIDEEAFDKIMSYIDMAGISDEVK
                     ILTGGNGDKSKGYFIEPTIILTENPHFITMEEEIFGPVVTVYLYEDEKFDEILKLCDE
                     TSPYGLTGSIISRDRYAIAKACRVLRYSAGNFYINDKPTGAMVGQQPFGGSRASGTND
                     KAGSLINLIRWVSPRAIKETFIPPACFRYPYMD"
     gene            4572..6110
                     /locus_tag="PLZ75_07925"
     CDS             4572..6110
                     /locus_tag="PLZ75_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOS72360.1"
                     /translation="MLVRTFAASVTGIDAVRVTIEVDVSRGIRFFLVGLPDVAVKESQ
                     QRIESAFRSIGYHWPGKQVVINMAPADIRKEGSSYDLPLAVGILAADGKIPGNGLESF
                     MLTGELSLDGSVQAVRGVLPMALRARNEGFRGMIVPEKNAAEAAVVDGLDVYGMAGLG
                     DVVNFLSGRGQYRPLKLDLKELFDGGSARYDVDFADVRGQENVKRALEVAAAGHHNVL
                     MTGPPGSGKTMLARRLPTIMPPMTPEEALETTKIHSVAGKIGIHSSLITTRPFRNPHH
                     SISDASLIGGGTYPQPGEVSLGHNGVLFLDELPEFRRSALEVMRQPLEDRIITISRSR
                     TSCVFPAGFMLVASMNPCPCGFFNHPSRECVCSPGMVQKYLNRISGPLLDRIDIHIDV
                     TPVRYDRLSGSPGTERSETIRERVVRAREIQAERFAGMEGIYSNAQMTPAVLRRYCHP
                     GEAGGRLLRAAMEMRGLSARAHDRILKLARTIADLAGAEYIAAEHIAEAINYRNLDRS
                     GFYG"
     gene            complement(6101..7426)
                     /locus_tag="PLZ75_07930"
     CDS             complement(6101..7426)
                     /locus_tag="PLZ75_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOS72361.1"
                     /translation="MLYRFLQFILPPFTFRVYYRRIFYSNLNRVPLDKPILFVGNHQN
                     SFLDGILVGSYLTRPIHFTMRADMFRNRLASFLLRELNVSPVYRLEEGYGNVHRNTEA
                     FNAIYDILRKNGNYIMFSEGVCIQEKRIHKLRKGTARLAFFAAEQYGLDVHVVPVGIN
                     YTYPAQFRKEVMISFHDAFSIREMIDLYKSSPAKAFLRFNEKCTEGLKKEVIIIEDPK
                     NDWLAEELLKMERNNIVHPFFRWMFRSDDRRMAEKLVADRINHLSQENSQERDSLEAR
                     VREYTGLLSREGLKDVNIARKADLGWLRYFALIAGIPVFIAGYVLNLIPFLVPPALTK
                     KLIKDSRFTSGFYVSAGTVLCLIWFLLGFAIAVVKAGWTGIPIAAAIPLLGYLALFYR
                     ENFRERLDRFRFVMKSLRNKSLIQDLRNRRMEIQKVLNSIEIPAVTVYP"
     gene            7625..>7815
                     /locus_tag="PLZ75_07935"
     CDS             7625..>7815
                     /locus_tag="PLZ75_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72362.1"
                     /translation="MELINRLRHEMQKEDLMFAYRGLVTLENSVPLIMLIEREMETAE
                     YGPVARKRLFMFVVESLQNV"
BASE COUNT         2082 a         1730 c         1934 g         2069 t
ORIGIN      
        1 atacagaaga atcagaattt cataacgctt ttatccttta caaacagcag gcaataataa
       61 taattctata aacatgaggg tatctttttt ttacggatgc ccggtataaa aactggaaaa
      121 aatgaaatgg cagggcagag aacgcagtga taatgtggaa gaccgcaggg gcatgggtac
      181 gggaggccgg atggcaatag gcggaggtat agggacggtt ctgatagtac ttattgtact
      241 gttgctggga ggagatccct cggcacttct ggatgatccc ggcacgatga tccgtacgga
      301 gcagtacacg ccttctgcgg aagaggagga actggcttat tttgtgagtg ttgtgctgaa
      361 agatactgaa acggtatggg atgagatttt ccggaaatcg ggacgtacct acagacaacc
      421 aaagcttgtg cttttcagcg gccaggtgga atcggcctgc gggtatgcaa gtgcctccag
      481 tggtcccttc tattgcccgg gtgatgagaa agtatatatt gatctgaact tcttttcgga
      541 acttcagaac aggctgggag ctcacggtga ttttgccgtt gcctatgtaa ttgcgcacga
      601 gatagggcat catgttcaga acctgctggg cattcttgac caggtaaact cacagagggc
      661 cggactgaac caggtccagt ccaataagct gctggtacgc ctcgaactgc aggcagattt
      721 cctgtcggga gtatgggcgc attatgccga cagaatgctt aatacacttg agaaaggaga
      781 tgccgaagag gcaatgaacg cagctgcctc cgtaggagat gatgttctgc agaaaaaata
      841 ccagggacgt gtttatcccg attcatttac gcacggaaca gctgcccagc gcaaggaatg
      901 gttcagaaaa ggatttcagt ccggtgacct tcgtcaggga gataccttca gcgcagattt
      961 ataatagccg cttttccttc agccagtctg ttcctgcatt tatttttccc ctgattacct
     1021 gttgccggct tccctgtcgg catcagcaat catcttttca tttgctgtga taaggaattc
     1081 cacccgccgg ttctgtgcac ggccctcttc ggtggcattg tcatatttcg gcatggtaag
     1141 tccgaaaccc tttattgtca tgcgttcact gccgatccct ttttgtgcaa gatatccgga
     1201 tactgccgaa gcacggtttt cagaaagtgt ctgattatag gccttgcttc ccttgttgtc
     1261 agtatgtccc tgaagttcaa tgtccgtatc gggataggct tccaggaccg taacaagcct
     1321 gtcgagattg ctttttgcct cagcggaaag ttccgacttg tcaaaggcga acaggatatt
     1381 gctgctgaat tcaacaacaa tgccttctcc cactctttcc acctttgcat cgggaacctt
     1441 attctttatc tcctcagcct gcttgtccat cttgttcccg ataattgcac cggtggttcc
     1501 tccaacggca gcaccgacaa ctgctccaag tgccgtgttt ccggacacct tgcctataac
     1561 cgcacccagc gcaccgccgg cagcagttcc gatagcagcc cccttctggg ctttattcat
     1621 tgaagcacaa cctgaaatga tcagggatgc ccctgcaagt accaaaaaaa tatttcttac
     1681 ctttttcata attgtgattt tgatatttag ctgtttatct gtgattctgt ctgttctttt
     1741 ataattatcc gttcctgtga gtcagtggca gccatcgcaa aacctgcagc acaattttcc
     1801 ctcttcctct gtgatgttat tgctgaccgg cagactgacc agttcctcat tgtattgcca
     1861 ctagctcaaa atgctaaaaa attatgcgaa tttagtaaat ttaacgatac gccttgaatt
     1921 ccggagaaat ttatgtaaat ttaaatctgg gcaatatctt ttcggagaaa actgaataaa
     1981 aggaggatat ctgaaaggag caaaatttaa tttattaact aaaatcactt accatgggat
     2041 taatttcatt tattaagaat gccggagaga agctttttaa atccggggaa aagggagaac
     2101 aggaaaaagc ggaactgatc aggactcata tcggcaggtt tggttttgac acaagcagga
     2161 ttcaggtgac agtggataag gacagggtaa tattgtcggg gaccgttgat acaaccgaca
     2221 acaagaacaa ggttattgtt acggccggga atgttgacgg aattgctgct gttgaagaca
     2281 ggatcgttgt cactgagccg gttaggtctg caccgcctcc tccggaaaaa cagttttaca
     2341 cggttaagaa aggagattac ctttcaaaaa ttgcaaagga agtttatggc aatgccaata
     2401 aataccatat aatttttgag gctaacaggc cgatgctgaa agatcccgat ctgatctatc
     2461 ccgggcaggt actggttatc cctcctcttg aagaataata tccttattct gtgagaataa
     2521 aagtttctcc ggcacttcct catgtcaccc ctgcaattgg caggactgac agctccttct
     2581 gcatagcctt ccttatctgg tttccggcgt gtagatgaat tatctgactt ttaacatgtt
     2641 ttatgtaagt attttattat attattgtta gaaaattgac tgatatgagt aatgctgtat
     2701 ttaatttcga acaaccacga aatgagccgg tattatctta tctgccggac agtgacgaac
     2761 ggaaatttct tgaagaggaa ctgaatctac aaagcaataa tgtaatcgat attcccctga
     2821 taatcggagg gaaagagata cgcacgggga aaacgggacg gattgtcaag cccggtgatc
     2881 acggacatct tcttgcgaac tatcatatgg caactgaaaa ggaagtcagg ctggccataa
     2941 atgctgcact tgacgcaagg gagcaatggc ttgaactgga atggattgaa agggctgcaa
     3001 taatgtcgaa ggcggctgag ctgatctcaa aaaaatacag atacaggctt aatgctgcca
     3061 caatgcttgg ccaggggaag aatgcctatc aggcagaaat cgattcagca tgtgagctga
     3121 tagatttcct gcgcttcaat aactatttcg cctatctgat attcaccgga cagcccctgt
     3181 cggaagatca ttccatgaac cggatcgaat accgtccgct ggaaggcttt gtctatgcgg
     3241 tcactccttt caatttcacg gcaatagcat ccaatctgaa caacagtgtg gcactgatgg
     3301 ggaataccac cgtgtggaaa ccggcatcaa ctgccgtgtt gtcaaattat tacctgatgg
     3361 aggtaatgaa ggaggccgga ctgcctgacg gagtcataaa cttcgtgccg ggttccggtc
     3421 cgctgataag cagtgtggtt ttcgggcaca gggaaatggc aggaatccat ttcaccggat
     3481 caggcagtac cttcaccacg ctgtggaagc aaatctcgga taatctgccg ctttaccgtt
     3541 catttccgag aatagttggt gaaacaggag gcaaaaactt tattgttgca cacagttccg
     3601 cggatcccct tgcccttgca acagcagttg taagaggtgc tttcgaatat cagggacaga
     3661 aatgttccgc agcttcaaga gcctatattc cccggtcaat atgggaagaa acatcggaat
     3721 acatttatag gatggtatct gatatcagga tcggtgacgt gacggatctg aacaatttcg
     3781 taaatgccgt gattgatgag gaggcatttg acaagataat gtcctatatt gatatggccg
     3841 ggatatcgga tgaggtgaaa atcctgacag gtggaaacgg cgacaaatcg aagggctact
     3901 tcattgaacc aaccattatt cttacagaaa atcctcattt cataaccatg gaggaggaaa
     3961 tattcggacc ggtggtaact gtctacctct atgaggatga gaaatttgat gagatactga
     4021 agctgtgtga cgaaacatca ccctatggcc ttaccggttc catcatcagc agggacaggt
     4081 atgcgattgc aaaggcctgc agggttctgc gctattcagc cggcaatttt tacatcaatg
     4141 acaaaccgac gggggcaatg gtcggtcagc agcctttcgg aggctcacgt gcatcaggaa
     4201 caaatgacaa ggcaggaagc ctgataaacc tgatccgctg ggtaagcccg agggctataa
     4261 aggaaacatt cattcccccg gcctgtttca ggtatcctta tatggactag ccggcaggca
     4321 gggaaaactg ttctgacacc cttcccctga aggcattccc tgttccggca gctctttcat
     4381 caaatatacg gaagtctttt tccccttgac gggcctggga ctttccctgc aggttcagca
     4441 acaccgcatt tatgaatgga ttctgttgct tattctgttc atcaccggtt agcgttcttt
     4501 cattttgtta tttcccccgc attttttata tttgattctg tatttccgga tgtgctaata
     4561 aacagaatat tatgctggta aggacatttg ccgcctcggt gacaggcatt gatgctgtaa
     4621 gggttaccat tgaagtggat gtgtcgcgcg gtatcaggtt ttttcttgtg ggccttcctg
     4681 atgttgcggt aaaggagagc cagcagagga ttgaatcggc tttccgttcc atcgggtatc
     4741 actggccggg gaaacaggtt gttataaaca tggccccggc agacatacgc aaggagggat
     4801 catcctacga ccttcccctg gccgtgggaa ttctggcggc tgacgggaaa attcccggaa
     4861 acgggcttga atcattcatg cttaccggtg aactgtcgct ggatggttcg gtacaggctg
     4921 tcagaggtgt gcttcccatg gctctgcggg ccaggaatga aggattcagg ggtatgattg
     4981 tcccggaaaa gaatgccgcg gaagcagccg tggtggacgg actggatgta tacggaatgg
     5041 cgggcctggg tgatgtggtg aattttctca gcggcagggg gcagtacagg ccgctgaagc
     5101 ttgacctgaa ggagttattc gacggtggct cagcgagata tgatgttgat tttgcggatg
     5161 tcaggggtca ggaaaatgta aagagggccc tggaggtggc agctgccggt catcataatg
     5221 tgctcatgac cggaccgccg gggtcgggca agacaatgct ggccagaagg ctgcccacca
     5281 tcatgcctcc catgactccc gaagaagccc ttgaaacgac aaagatccat tcagtagcag
     5341 gtaaaattgg tatccattca tctcttatca ccaccaggcc tttcagaaac ccccatcatt
     5401 caatttcaga tgcatcactg atcggtggcg gtacataccc tcagccggga gaggtatccc
     5461 tgggacataa cggtgtcctg tttcttgatg agcttccgga attccggcga agtgcacttg
     5521 aagtgatgag gcagccgctg gaagacagga taatcacaat ttcaagatcc agaacgagct
     5581 gtgttttccc ggcaggcttt atgctggtgg cttcaatgaa tccctgtccc tgcggatttt
     5641 ttaatcatcc ttcaagggaa tgtgtgtgct ctcccgggat ggttcagaaa taccttaacc
     5701 gtatatcagg acctctcctg gacaggattg atattcacat tgatgtgact ccggtcaggt
     5761 atgacaggct ttcagggagc cccggaactg aaagatccga aacaataagg gaaagagttg
     5821 taagggcaag ggaaattcag gcggaaagat ttgccggaat ggagggaata tattccaatg
     5881 cccagatgac cccggcagtg ctgaggagat actgccatcc cggcgaagcg ggaggaaggc
     5941 ttctgagggc tgctatggaa atgaggggat tgtcagccag ggcccacgac cgtattctga
     6001 aactggcacg aacgattgcc gatctggcgg gagctgaata tatagccgcc gaacacattg
     6061 cagaggcaat caattaccgt aacctggacc gcagcggatt ttacggataa acggtaacag
     6121 caggtatttc aatgctgttc aggactttct ggatttccat gcgtctgttt cggagatcct
     6181 gaatgagaga tttatttctg agtgatttca ttacaaatct gaatctgtca agcctctccc
     6241 ggaaattttc cctgtaaaaa agtgccagat accccaggag aggaatagct gctgcaatcg
     6301 ggattcctgt ccatcctgcc ttcacgacag cgattgcaaa accaagaaga aaccatatca
     6361 ggcagagaac cgttcctgct gaaacataaa aacctgaggt gaaacggctg tcctttatta
     6421 atttttttgt gagagcaggc gggaccagaa agggaatgag gtttaaaaca taacccgcaa
     6481 taaaaaccgg aatcccggcg ataagggcaa agtacctgag ccatcccaga tctgcttttc
     6541 ttgctatgtt cacatccttt aagccttcgc gggagagcag accggtatat tccctgaccc
     6601 ttgcctcaag ggaatcccgc tcctggctgt tttcctgaga aagatggttt atcctgtcag
     6661 ccacgagttt ctcggccatt cttctgtcgt cagaccggaa catccagcgg aaaaagggat
     6721 gcactatatt gtttctttcc attttcagca attcctcggc aagccagtca tttttcggat
     6781 cttcaattat aatgacctct tttttcagac cctcagtgca tttttcattg aatctaagaa
     6841 atgccttagc gggactgctt ttgtataagt caatcatttc ccttatgctg aaggcatcat
     6901 ggaagctgat catcacttct ttcctgaact gtgcaggata agtataattg ataccgaccg
     6961 gtactacatg cacatccagt ccgtattgct cagcggcaaa gaatgcgagc ctggctgtac
     7021 cctttctgag tttgtgaatg cgcttttcct gaatacagac accttctgag aacatgatat
     7081 aatttccgtt ctttctgaga atatcataaa tggcattgaa tgcctctgta ttcctgtgaa
     7141 cattcccgta accctcctca agcctgtaaa ccggggaaac attcagttcc cgcagaagga
     7201 agctggccag acggttgcgg aacatatcgg ccctcattgt aaagtgaatg ggccgggtaa
     7261 gataggaacc gaccaggatt ccgtccagga atgaattctg gtgattgccc acaaaaagaa
     7321 tgggtttgtc aagtggcacc ctgttcaggt tggaataaaa aatccgcctg taataaaccc
     7381 tgaatgtgaa aggtggaagt ataaattgaa gaaacctgta aagcatggta tcccggtaga
     7441 ttattaaagc taaattaaaa ataaaaacga tgtgccggac aggttttggg ttaaatctgt
     7501 atcaggatca gaaataaata tttgtctgcc attgttgata agtcctgatt tctttctgtt
     7561 tatccatatt gcagaaatga ggttttcact aaatttatcg tgaaattatt cacagggtta
     7621 ttctatggag cttattaacc ggctgcgcca tgaaatgcaa aaggaggatc ttatgtttgc
     7681 atacagaggc ctggttacac ttgagaactc agttcccctt ataatgctta tcgagagaga
     7741 gatggagact gccgaatacg ggccggtcgc caggaaaaga ctgttcatgt tcgttgttga
     7801 aagtcttcag aatgt
//
LOCUS       DAOSPX010000138         7805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8783_length_7805_cov_4.165677, whole genome shotgun sequence.
ACCESSION   DAOSPX010000138 DAOSPX010000000
VERSION     DAOSPX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8783_length_7805_cov_4.165677"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   3..903
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3..33
                     /rpt_unit_seq="gtcgcaccccgtgcgggtgcgtggattgaaa"
     gene            complement(1254..2330)
                     /locus_tag="PLZ75_07940"
     CDS             complement(1254..2330)
                     /locus_tag="PLZ75_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HOS72363.1"
                     /translation="MKDSISRRKFIGRSIGISTGALVLPSAEAFGMQVKSGSTIPLII
                     TSHANDTGRKALEEGWKILAAGGSAVTAVETAANIIELDPEDTSVGYGGLPNEKGVVQ
                     LDASFMDGRTYSAGAVACLENIKTPSSVARLVMQRTNHVLLTGAGALEFAKAWGFREE
                     NLLTEKARKIWLRWKEEMSAIDDWGPPEHLKDQQKLESWWQDFPEIEHHYGTTNVLAI
                     DKNGDIAGCTTTSGLSFKLNGRVGDSPIIGAGLYVDNEVGAAAATGRGEDVIKSCASY
                     FMVLRMKDGRSPQQACEDALHMITDRYKKVNPDYFPSEKFVAINKYGETGCASMKGSV
                     NPQMSVMTGRGYTKYEGTIAFPGK"
     gene            complement(2341..3108)
                     /locus_tag="PLZ75_07945"
     CDS             complement(2341..3108)
                     /locus_tag="PLZ75_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOS72364.1"
                     /translation="MKKTLTLLIMLAAAAASNAQVKIIAHRGASWLAPENTVASARLA
                     WEKNADAVECDIYLSKDNKIIVIHDSNTKRTTGEDHVVSETNSKVLRALDAGSFKDEK
                     YRGEKLPFLSEIIKTVPKGKELVVEIKCKSEILPYLEKAVRKYQNKIVFTFICFDFQT
                     ITDTRKTFPGNACYWLCSNAELLDKTFSKVGPAGLEGVSLSWNIITEDVVKKARELGL
                     EVYSWTVDDPAEAKRLIGLGVEGITTNRPGWLNEQIF"
     gene            complement(3197..3931)
                     /locus_tag="PLZ75_07950"
     CDS             complement(3197..3931)
                     /locus_tag="PLZ75_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOS72365.1"
                     /translation="MKFLRITSETVFSRLRVYIISALFILTVSCSPLRVYRDLPEVKA
                     WEPEIEKLTALGNTEQYAEDAIVFAGSSSIRLWKTLASDMEPYNVIQRGYGGAKLSDY
                     GVYANRIFGNIPGRALVLFVANDITGSEKDKSPGEVKKLFLHIVKTFRKTHPGAPVFW
                     IAITPTSSRWKAWPEIKEANELIRETCEKGKNLYFIRTDFAFLNDQGQPKDELFMADR
                     LHLNEDGYRIWTDLIKNELNKVLTSK"
     gene            complement(4172..5326)
                     /gene="lysA"
                     /locus_tag="PLZ75_07955"
     CDS             complement(4172..5326)
                     /gene="lysA"
                     /locus_tag="PLZ75_07955"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOS72366.1"
                     /translation="MITNETTEKFRKLKLPTPFYYYDLEVLKTTLDVIKKKASESGFK
                     LHYAVKANHNPRILSLIASYGLGADCVSWNEIDAALKAGFRASEIVFAGVGKSDNEII
                     NAIEADIFCFNCESIPEMEVINTFAARLGKKARIALRINPLVDAHTHKYITTGIAESK
                     FGINTWELEEVLKKLTDMKNLELTGIHFHVGSQITRLSVFKTLCNHINELQEWFSVRN
                     SELKIINVGGGLGINYDNPEELPRFDDYFSLIRKFVDLRPGQTIHAEPGRSIVGQCGS
                     LISKVLYVKNGTNTKFVIIDAGMTDLIRPALYQAHHQIENLTSAGETENYDVVGPICE
                     SSDTFARYIELPETRRGDLIAIRSAGAYGEVMASRYNLRDLPQVVFSDEI"
     gene            complement(5331..6656)
                     /locus_tag="PLZ75_07960"
     CDS             complement(5331..6656)
                     /locus_tag="PLZ75_07960"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOS72367.1"
                     /translation="MKITKIMKFGGTSVGSPERIKALIPLITGNEQKIVVLSAMSGTT
                     NSLVEITELLYSGDKQTASDRIESLRTRYHKVVDELYETDEYKNTGHEMIDSHFNSMG
                     NIAARPFTRLQERAIVGEGELISTALLHKLLLERGIKSKLIPAVNFMRIDKDGEPDYY
                     YIEENLKRELRRCDSKSVLITQGFICRNVKGEIDNLKRGGSDYTASLIGSVLKVDEIQ
                     IWTDINGFHNNDPRFVENTEVIRELSFDEAAELAYFGAKILHPSSITPARDNNIPVRL
                     KNTLHPEDEGTLITSSSELMDYKAVAAKDGISVIRIRSYRMLMAYGFMRKVFEIFEAY
                     KTPIDMITTSEVSVSITIDNPRYIREIAADLSELGTVETEEDQTIVCIVGDFRMKKTG
                     SAPEIFEALDTIPLKMISYGGSDISISLLIDTSHKKEALRLLSKHLFPH"
     gene            6952..7035
                     /locus_tag="PLZ75_07965"
     tRNA            6952..7035
                     /locus_tag="PLZ75_07965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6986..6988,aa:Leu,seq:cag)
     gene            7138..7221
                     /locus_tag="PLZ75_07970"
     tRNA            7138..7221
                     /locus_tag="PLZ75_07970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7172..7174,aa:Leu,seq:gag)
     gene            7563..>7805
                     /locus_tag="PLZ75_07975"
     CDS             7563..>7805
                     /locus_tag="PLZ75_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72368.1"
                     /translation="MKRFLLFPVTLLLCFQLSGQRGTIVSTFPMATLSPEEIMLNLKN
                     NLDGTAYTLAGLFIYQKYSVTAVKVIYKTINGKGQPT"
BASE COUNT         1889 a         1827 c         1699 g         2390 t
ORIGIN      
        1 atgtcgcacc ccgtgcgggt gcgtggattg aaactatgaa acgctcaaat caatatcaaa
       61 atcaacttgt cgcaccccgt gcgggtgcgt ggattgaaac aaataattgg aacacggctt
      121 atggatgggg taagtcgcac cccgtgcggg tgcgtggatt gaaacatgat aacacgttca
      181 atagatgtcg aagtaatatt gcgtcgcacc ccgtgcgggt gcgtggattg aaacctttta
      241 gccttacccc tgactgcaat gacctccagg tcgcaccccg tgcgggtgcg tggattgaaa
      301 ctcttgttga gcggtcaggg aatctcaaaa ggattggtcg caccccgtgc gggtgcgtgg
      361 attgaaacat aactaaaaag gtaaatcatt atcatccatc acggtcgcac cccgtgcggg
      421 tgcgtggatt gaaacatcac aacacaatgc ttaattgttc cagggataga gtcgcacccc
      481 gtgcgggtgc gtggattgaa accgaattac cgatttgcag gacttgaaag caaaattgtc
      541 gcaccccgtg cgggtgcgtg gattgaaact aaaataggaa ataattgtca gattgatagt
      601 aattgtcgca ccccgtgcgg gtgcgtggat tgaaactggc aataatacgt gatgctatct
      661 tggttggtga cagtcgcacc ccgtgcgggt gcgtggattg aaactctttt tggatttgtc
      721 aagcaataac gccagtcctt gtcgcacccc gtgcgggtgc gtggattgaa acctgttgat
      781 caatcctcag ccccttgcct gcatcgtcgc accccgtgcg ggtgcgtgga ttgaaacatt
      841 agttgtcttg acaaatgttg gaatcttttc agtcgcaccc cgtgcgggtg cgtggattga
      901 aaattttcaa ttaatttcga cggctttttt tacatactaa ttccggcaca gtcagcaata
      961 gctttacttt tatctgcccg ttgttatgca attaattcat ccagaagtaa tgttcccgaa
     1021 cgtcgcgcta aacctaattg cttattatct tcatacaaat aatcagtttt agccggatat
     1081 cgtatcaaaa cgtgccccga caggtgccta atggtaatct gttctttctt tacggtcttt
     1141 acttatggtc atggcaaact ctggctgaat ctctctaagt gtgaaacttc gtttagagca
     1201 gcgttgtatt tgatgtgatc aagacataaa tcctgaatta ccctgacgga tacctatttc
     1261 ccgggaaagg caatggttcc ttcatatttt gtataacccc tgccggtcat aaccgacatc
     1321 tgagggttga cgctgccttt cattgaagca catcctgttt caccgtattt attgatggca
     1381 acgaatttct cgcttggaaa ataatccgga ttaacctttt tgtaacggtc tgtgatcatg
     1441 tgcagggcat cctcgcatgc ctgctggggg cttcttccat ccttcattct cagaaccatg
     1501 aaatatgatg cacaagattt tatgacatcc tccccacggc ctgtcgcagc agcggcacca
     1561 acctcattgt cgacatacag acccgctcct atgatgggag aatcgccgac acggccgttt
     1621 agtttgaagc tcagtccgct ggtggttgtg catccggcaa tatccccgtt tttatcaatg
     1681 gccagaacat tggttgttcc gtagtgatgt tcaatttcag gaaaatcctg ccaccaggat
     1741 tccagttttt gctgatcctt cagatgctcc ggaggtcccc agtcatctat ggcactcatt
     1801 tcttccttcc acctgagcca tatcttccgg gctttttccg tgagaaggtt ttcttcacgg
     1861 aagccccatg cttttgcaaa ttccagcgct ccggcaccgg taaggaggac gtgattggtt
     1921 ctctgcataa caagtcttgc cacggaggat ggagttttta tattctccag acaggctaca
     1981 gctccggcag aatatgtcct gccgtccata aaagatgcat cgagctgaac aactcccttt
     2041 tcattgggca atcccccgta acccacactt gtatcctcgg gatccagttc aataatgttg
     2101 gcagctgtct ccacagctgt gactgcagaa ccacctgcag ccaggatttt ccatccctct
     2161 tcgagggctt tcctgccggt gtcgttggca tgacttgtaa taattagcgg tattgttgaa
     2221 ccgcttttta cctgcatgcc gaaagcttcg gctgaaggca gtacaagtgc accggtactg
     2281 atacctatgc ttctgccgat gaattttcgc cgggaaatac tatctttcat aacacttttt
     2341 ttagaatatc tgttcattca gccatccggg gcggttggtg gttatcccct ccacacccag
     2401 accgataagg cgttttgctt ctgcgggatc atccactgtc caggaataaa cttcaagacc
     2461 aagttccctt gcttttttga caacatcctc agtaatgata ttccatgaaa ggcttacacc
     2521 ttccaggccg gcaggaccga ccttactgaa tgttttatcc aagagttctg cattgctgca
     2581 cagccagtag caggcgttac ctggaaaggt cttcctggta tcggttatag tctggaaatc
     2641 aaagcagata aatgtgaaaa caatcttatt ctgatatttc cttactgctt tttcaagata
     2701 tggaagtatt tcacttttac attttatttc cacaacgagc tctttcccct tcgggactgt
     2761 ctttattatc tctgacagga acggcagctt ttcacccctg tatttttcat ccttgaagga
     2821 accggcatca agtgcacgga gaactttcga attggtttca cttacaacat gatcctcgcc
     2881 tgtagttctt ttcgtgttgg aatcatgaat gacgataatc ttattgtcct ttgaaagata
     2941 gatgtcacat tccacggcat ctgcgttctt ttcccatgcc agtctggctg aggccacagt
     3001 gttttccgga gctagccatg aggcgcccct gtgggcaatt atcttgacct gtgcatttga
     3061 tgcggcggct gctgcaagca taatcagtaa tgttaatgtt tttttcatca gaatataatt
     3121 tgttttattg ttttgatgat ctgttccact cagcggtaga atgtcttata ccggcagacc
     3181 ggaatatgcg ggttgtttat ttgctggtca gtaccttgtt cagttcgttt ttgattaaat
     3241 cagtccatat cctgtagcca tcctcattga gatggagcct gtcggccata aaaagctcat
     3301 ctttgggctg tccctgatca ttcaggaatg caaaatccgt tcgtatgaaa tacaggtttt
     3361 ttcctttttc gcatgtttcc cggatgagtt cattggcttc ctttatttcc ggccatgcct
     3421 tccagcggga ggatgtggga gtgatggcta tccagaatac cggtgcaccc ggatgtgtct
     3481 tccggaaagt tttcacaata tggaggaaaa gcttttttac ttctccggga ctcttgtcct
     3541 tttcacttcc ggtaatgtca tttgcgacaa acagcacaag tgctcttccc ggaatattcc
     3601 cgaatatcct gtttgcataa accccgtaat cactcagttt tgcacccccg tagcctctct
     3661 gtatcacgtt gtagggctcc atatcagagg caagcgtttt ccacagcctt atgcttgaac
     3721 tgccggcaaa gacaattgca tcttcagcgt actgctctgt attgcccagt gctgttaatt
     3781 tctcaatttc cggttcccat gccttaacct ccggcagatc gcggtaaacc cgcaggggcg
     3841 aacacgagac agtcaggata aaaagagctg aaataatgta tacgcgtaag cgggaaaata
     3901 ctgtttcaga agtgattctt aaaaatttca tgaagctgaa gtaattcttt aaagcctaaa
     3961 tatacctcat atactccaat agtataaaaa aattcagaaa ttcttttatt cctggacgtt
     4021 tacccaaact tgcctgacgg tttagtgcct gatggtttaa caggttgctg atggcctgat
     4081 ctgattgcct gccggctgct aagaacggac cggaattgct gccagtctga cttaccggcc
     4141 gcaccggaca gacttcctgt aatccgcttt gttatatctc atctgaaaag accacctgcg
     4201 gaagatccct gaggttatat cttgatgcca tcacttcccc gtatgccccg gccgatctta
     4261 ttgcaataag gtcacctctt ctggtttcgg gaagttcgat atatcttgca aaggtatctg
     4321 atgattcaca tattggtccc acaacatcat aattttccgt ttctccggcg gaagtaaggt
     4381 tttctatctg gtgatgtgcc tggtaaagtg ccggacggat aaggtctgtc attccagcat
     4441 caataatcac gaattttgtg ttggtgccgt tttttacata caggactttc gagatgaggg
     4501 agccgcactg tcccactatt gatctgcccg gttccgcatg aattgtctgg cccggtctga
     4561 gatcaacgaa cttgcgtatc agtgaaaaat aatcgtcaaa cctcggtaat tcctcaggat
     4621 tgtcataatt gatacctaat cctcctccca catttatgat tttcagttcg gaatttctca
     4681 cggaaaacca ttcctgcagc tcgttgatat gattgcaaag cgttttgaag accgacagtc
     4741 tggtaatttg tgagccgaca tgaaaatgga ttccggtaag ctccaggttc ttcatgtctg
     4801 taagtttttt aaggacttct tccagttccc aggtgttgat tccgaatttg ctttctgcaa
     4861 tgcctgtggt tatatattta tgggtatggg catccacaag cggattgata cgaagggcta
     4921 tccttgcctt ttttccgagc ctggccgcga atgtgtttat cacttccatc tcgggtattg
     4981 attcgcagtt gaagcagaat atatctgctt ctattgcatt tattatttca ttgtccgatt
     5041 ttcctacgcc ggcgaataca atttcggaag ccctgaatcc tgccttcagt gcagcatcta
     5101 tttcattcca gcttacacag tcggcaccca gtccgtatga cgcaatcaga ctgagtattc
     5161 tcggattatg gttggctttt actgcataat gcagtttaaa tcccgattcg gatgctttct
     5221 ttttgatcac atccagtgtc gttttcagga cttccagatc atagtaataa aacggcgtgg
     5281 gaagtttaag cttcctgaat ttctcggttg tttcgttggt aatcatatta tcagtgagga
     5341 aataaatgtt tgctcagaag tctcagcgct tccttcttgt gactggtatc tatcagcaat
     5401 gaaatactga tatcgctgcc cccgtaagat atcattttaa gcggaattgt atccagtgct
     5461 tcaaaaattt ccggtgctga tcccgtcttt ttcatccgga aatccccgac tatacagaca
     5521 atagtctggt cttcctctgt ttcaactgtt cccagttcac tcagatcggc agcaatctcc
     5581 ctgatatatc gtggattgtc gattgtaatg gagacggaca cctctgaggt tgtaatcata
     5641 tcaatcggtg ttttgtaggc ttcaaatatc tcgaacactt tgcgcatgaa accataggcc
     5701 atcagcatac ggtacgacct gatccttatt accgatatcc cgtctttcgc cgccacagct
     5761 ttataatcca tcagttcgct ggatgatgta ataagggttc cctcatcttc aggatggagg
     5821 gtgttcttca gccggactgg gatattattg tcgcgggcag gggttatact tgaggggtga
     5881 aggatttttg ccccgaaata agccagttct gcggcttcgt caaatgaaag ctcgcgaatg
     5941 acttccgtgt tttcaacaaa cctcggatcg ttattgtgga aaccattgat gtcggtccat
     6001 atctgtatct catccacttt cagtacagat cctatcagtg atgccgtata gtcgcttccc
     6061 ccgcgcttga gattgtcaat ttcccctttc acgttacggc agataaagcc ctgtgttata
     6121 agaacgcttt ttgagtcaca tcttctgagc tccctcttca ggttttcctc tatgtaataa
     6181 taatcgggtt caccgtcttt gtcgatacgc atgaaattca ctgcgggaat cagttttgat
     6241 ttgattcctc tttccagcag aagcttatgc agcaaagcag ttgatatcag ttcgccctca
     6301 cctacaatgg ctctttcctg caggcgggtg aagggtctgg ccgcaatgtt tcccatggaa
     6361 ttgaaatgag aatcaatcat ctcatgtccg gtattcttat attcatccgt ttcataaagc
     6421 tcatcaacca ctttatgata gcgggtcctc agcgactcaa tccggtctga tgctgtctgt
     6481 ttatcaccgc tgtacaggag ctctgttatt tcaacaaggc tgtttgtggt tcccgacatg
     6541 gccgaaagaa ccacaatctt ctgctcattg ccggtgatta aaggtataag tgctttaatt
     6601 ctttcgggac tgccgaccga ggtcccgccg aatttcataa tcttcgttat tttcatttta
     6661 tttaagctgt tttaaaatct gcatttcagg aaatatgcct gtgaagggaa catttgattc
     6721 aggcccgctt cctgttaacc ggaatggttt ggggaatcct ggcagctgtt tatatatatt
     6781 ttccatggtc agtgtgcaaa aatatacttt tctgtaaatc atgattcctt attattaaat
     6841 tttttattat ttttgtcccc gcaattgata ggcgcagagc acggaaccgg aaacccaggt
     6901 cagaacgtac tgacaaccac tgattccgga tgtctgggcc tgagctgata ggcccagatg
     6961 gcgaaattgg tagacgcgct agtttcaggg actagtgtca gcaatgacat gcaggttcga
     7021 gtcctgttct gggcacagag cggcagaatg ccattcgttc tttatgttaa ttcaggttaa
     7081 aaattttcag gttaatacac tacgggatgt tccgtaccgt ggcaaaagcc tgttaatgcc
     7141 cagatggtga aattggtaga cacgccagct tgaggggctg gtgccggcaa cggtgtgcaa
     7201 gttcgagtct tgttctgggc acaatatact tcctcattgt ttctgtaaat aattaaagcc
     7261 ctttatttaa attatttgca cttccggccg gattccagaa acttatttgc actaccttat
     7321 cttttttttt taaaatcccg gtcctgttac gattaaccgg acagtaagtc aagctaaaac
     7381 ccgatgccaa agcacagggc tgaatatcgg tttcagctgc ttttccggtt cccgggcagc
     7441 agtctttaaa cagtacaata ataccataag aattactgga ttacattata attcagagtc
     7501 gttttttttg atctttttta aaaaagccgt aaattattct tttttaattt aaaccaaggc
     7561 ccatgaaaag atttctacta tttccggtta cattgttgtt gtgtttccag ctttcaggac
     7621 agcgcggcac cattgtcagt acctttccca tggctactct ctcacctgaa gagataatgc
     7681 tgaacctgaa aaacaatctg gacggaacag catatactct tgccggactt ttcatttatc
     7741 agaagtattc ggtaacagca gtaaaagtga tctacaaaac cattaatggt aaaggacagc
     7801 caact
//
LOCUS       DAOSPX010000139         7743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8866_length_7743_cov_4.769641, whole genome shotgun sequence.
ACCESSION   DAOSPX010000139 DAOSPX010000000
VERSION     DAOSPX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8866_length_7743_cov_4.769641"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2225)
                     /locus_tag="PLZ75_07980"
     CDS             complement(<1..2225)
                     /locus_tag="PLZ75_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOS72369.1"
                     /translation="MKKLFSLSLCVLLCLATGILSLQKASAQSSVPDPASHFGFEPGS
                     DYMLFNYDSLIDYLQKLDKASPMLRLVENGQTPMGRKMYIVFISSEKNIRNLDKLREI
                     NRELALNADLSDKKQAEYIRDGKVFFLATLSMHSTEVAPTQALPLIAHKLITSSDPLI
                     RRYLDDVVYMAVPSHNPDGMEMVVDNYNKYKGTQYDGASLPGVYHKYIGHDNNRDFIT
                     LTQEDTKAIARIYNLDWFPQVMIEKHQMGSTGVRYFVPPAHDPIAENVDAELWAWTGI
                     FGTNMLRDLTSAGFSGVGQKMIFDDYWPGATETCIWKNVIGMLTEAASVKEASPVYIE
                     PNELQASGKGLAEYRKSINFPLPWPGGWWRLGDIVKLEIESTLSTIKTSALFKNDILK
                     FRNEICKREVMKGRTEAPFYFIVPEDQADQSELIALVELMKEHGINAYTLQENFTLDG
                     ISLKAGDIVYPAAQPFRPFLKEVMEKQEFPIRHYSTNGELIRPYDVTSWSLPLHRGLI
                     SYEIKTRNEAFEKKLKPLSGSYYPLKQDYKLPAIFPATSNGSYKAAFIALQNGMNVSR
                     INEEIVIDGKIYGKGSFLVQGGNDALLNMLVKESRTAPGFVKNPDKLARIAVKLPRIA
                     LVETYFHDMDAGWTRYLFDSYKIPYTVLRPGEFEKTDFTADFDIVIFPSSGKNLLLNG
                     KPGTESSPAMSNYHPDYQKGMGKKGLENLLLFVNEGGKAISWGESADLFTGMLTLSKG
                     E"
     gene            2457..3344
                     /locus_tag="PLZ75_07985"
     CDS             2457..3344
                     /locus_tag="PLZ75_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOS72370.1"
                     /translation="MVHNHGKQGIDLSNAFKIGIILNLVFIAIEIIYGFLANSMALVA
                     DAGHNFSDVLALVFSWIAVKLSERKPTLKFTYGFRRSTILVTILNTVLLFVAVGFLLW
                     ETVRRIGEKQVIDSKLVMIVAAAGIVINGFTAWLFVEGKKHDLNIKSAFLHFLADTLV
                     SAGVVLAGLIMALTGINWIDSLMSFIIIAIILYNAYKLLIESVSLALDAVPENIDIRE
                     VSGYFSSLPEVIGIHDLHIWALSTNSAALTVHLETNIQTDAFFVHTIRKHLHEKFGIE
                     HATIQVEYGGQPGECYNCN"
     gene            complement(3399..3857)
                     /locus_tag="PLZ75_07990"
     CDS             complement(3399..3857)
                     /locus_tag="PLZ75_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOS72371.1"
                     /translation="MSRKIFLLLIVSFSFRFGFSQEYKGSVIRVIDGDTFVFQTEEGS
                     LIVRMHGIDAPERKQPFAAESADFLKQYLNREASVQISGTDRYGRSIGVLYVNKQDIN
                     LLSVRQGFAWQYKQYSSDKKYLVAEKLARKRKRGLWAVPDPLPPWEWRKK"
     gene            complement(4104..6956)
                     /locus_tag="PLZ75_07995"
     CDS             complement(4104..6956)
                     /locus_tag="PLZ75_07995"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOS72372.1"
                     /translation="MSKQPELILEEQLIAKLQELGYVLVYIPNESELLANLKSQLEKH
                     NNIIFSDKEFEKVLNIVSKGSVFEKAKTLREKQHIIRDNGDNLYFEFINTEQWCQNQY
                     QVTHQVSMEGKYKNRYDVTLLINGLPLVHIELKRRGLELKEAFNQVNRYQRHSFSSSY
                     GLFQYVQIFVISNGVNTKYYANNRFQSFKQTFFWTDKENKRLTNMLNDFASDFLEKCH
                     ISKMICKYIVLNETSKILMVLRPYQYYAVEALVDRVLHCRKNGYIWHTTGSGKTVTSF
                     KASQILTRLPKIKKVVFVVDRKDLDYQTTNEFNSFSKGSVDGTDNTKQLVRQFGDDTK
                     LIVTTIQKLNVAISKKRFQERMKPLQDERIVFIFDECHRSQFGETHQRIISFFRNIQM
                     FGFTGTPIFAENAVKNELGKKTTKELFGDCLHKYVITDAIRDENVLKFSVEYVGRYKN
                     RDSANEIDIQVEDIDVEELMDSPQRLEKITDYIIAHHSRKTHNREFTAMFCVSSIPAL
                     IRYYELLYRKKMLGEHDLKIATIFSYIANEADVDANGFIPEEISAAAEPEITYATNPH
                     TREKLDEFIEHYNQMFGSAFSTRDSQSFYNYYNDISKKVKERKIDILLVVNMYLTGFD
                     SPSLNTLYVDKSLRYHGLIQAYSRTNRILNELKSQGNIVVFRNLKNATDEAITLFSNK
                     DAIEEIIMKPYDDYVKAFNDAFDNLLQITPTVDSVNSLVTEDDELKFIIAFRELMRIK
                     NVLTSFSDFKWSDLSMTEQQFEDYKSKYLDLHDKVKSEHAKEKVSILEDVDFELELIH
                     RDEINVAYIIRLLIKLKANQKKEAEQIEKEIFNLLNTEMQLRSKKELIEKFIRENLPV
                     ILDTDDIPQAFEKFWAIEEERAFLKMVEEEKLSKEKTQAFIENYLYSEQEPIRDEILE
                     LLIYDKPTLLQRKTTGDRILKKIMDFVETFINGISG"
     gene            complement(6953..>7743)
                     /locus_tag="PLZ75_08000"
     CDS             complement(6953..>7743)
                     /locus_tag="PLZ75_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HOS72373.1"
                     /translation="MQSDYNLHTFLPNTYFEDIVDRYNFDRQLRLFLFDAIERIEIAL
                     RTKMIYHMSVSYGGLWYLDPAFFENTTITLNGGMIKTIHQNTLDELKKEFDRSQESFI
                     KDHRFRYPNKDADAWKTLEVASMGTLSKFYKNLKHQLPEKAAIAKEMGLNLHSELSSW
                     LEAITYVRNIIAHHSRLWSRNMVKRPVEKLNNPVGKWFVNPLLPVQVKKPFLIISCMV
                     YLCNKITPNNQIKSKIHELIKSNPSIPIYKLGFLNNWNKEPLWQ"
BASE COUNT         2009 a         1645 c         1585 g         2504 t
ORIGIN      
        1 tcttctcctt tgctcagggt gagcattccc gtaaacaggt ccgccgattc accccatgaa
       61 atagccttcc ctccttcgtt aacaaagagc agaaggtttt ccaggccttt cttccccatg
      121 cctttctgat agtcaggatg ataattgctc attgccggac tgctttcggt tcccggcttt
      181 ccgtttaaaa gtaaattctt tccgcttgac gggaatatca caatatcaaa gtcagctgtg
      241 aaatcagttt tttcaaactc acctggccgc aggacagtat agggtatttt atagctgtcg
      301 aagaggtagc gtgtccatcc ggcatccatg tcgtggaaat aggtttcaac cagggcgatt
      361 cggggcagtt tcaccgcaat ccgtgccagc ttatcgggat tcttcacgaa tcccggtgcg
      421 gtcctcgatt cttttaccag catattaagc aaggcatcat taccgccctg gacaaggaaa
      481 cttccctttc cgtatatttt gccgtcaata actatttctt catttattct gcttacattc
      541 attccgttct gtaaggcaat aaaagcggct ttatagcttc cgttgctggt tgcagggaat
      601 atggcgggaa gtttataatc ctgtttcaga ggataatatg aaccgctgag aggtttcagc
      661 tttttctcga aagcttcgtt ccgtgttttt atttcatagg atatcagtcc cctgtgcagg
      721 ggaagggacc agcttgtgac gtcatatggc cgtatcagct cgccgtttgt gctgtaatgt
      781 ctgatcggga attcctgttt ttccattacc tctttcagga aaggccggaa tggctgtgct
      841 gcggggtaaa cgatatcacc cgccttaagg ctgatgccgt cgagagtgaa gttttcctga
      901 agcgtgtaag cattgattcc atgttccttc atcagttcaa caagggcgat cagttcgctc
      961 tggtcggcct gatcttccgg aacaatgaaa taaaagggag cttcggtcct gcccttcatc
     1021 acttcccgtt tgcatatctc gtttctgaat ttcagaatat catttttaaa cagggctgaa
     1081 gtttttattg tcgaaagggt ggattcaatc tcaagcttta caatatcacc cagccgccac
     1141 catccgccgg gccatggaag agggaaattg atgctcttcc tgtattcggc gagtcctttt
     1201 ccgctggcct gaagttcatt tggttctatg taaacgggtg atgcttcctt cacgctggct
     1261 gcttcggtaa gcattcctat cacatttttc catatacatg tttccgttgc acccggccag
     1321 taatcgtcaa atatcatttt ctgacccact ccggagaaac ccgcgctggt aagatcgcgc
     1381 agcatgtttg tgccgaatat tccggtccag gcccatagtt ccgcatccac attttccgcg
     1441 atgggatcat gtgccggggg aacgaaataa cgcacacctg tcgatcccat ctgatgtttt
     1501 tctatcatca cctgaggaaa ccagtcgaga ttatagatac gtgcaattgc ttttgtgtcc
     1561 tcctgtgtca gtgttataaa atcccggtta ttgtcatggc cgatatattt gtgatacact
     1621 ccgggcaatg aggcaccatc atactgtgtg cccttatatt tgttgtagtt gtccacaacc
     1681 atttccatac cgtcggggtt atggcttggc acggccatat ataccacgtc gtccaggtat
     1741 ctcctgatga gcggatcaga tgaagtgatc agtttgtgag cgatgagagg cagtgcctgt
     1801 gtgggtgcca cctcggtgga atgcatcgac agggtggcca ggaagaatac cttgccgtcc
     1861 cttatgtatt ccgcctgttt tttatctgaa agatctgcat tcagggccag ttccctgttt
     1921 atttcacgga gtttatcaag gttgcggata tttttttccg aagagatgaa tacaatgtac
     1981 atcttcctgc ccatgggagt ctgtccgttt tcaacgagcc tgagcattgg cgaagccttg
     2041 tccagtttct gaaggtaatc gatcagactg tcgtagttaa agagcatgta atccgatccc
     2101 ggttcaaagc cgaaatggga ggcaggatcc ggaacagatg actgggctga tgctttctgc
     2161 agtgataaga tgcccgtggc aaggcaaagc aaaacacata gtgaaaggga gaatagtttt
     2221 ttcatgattg agggtcctgg gttttaattt agccagataa atgtacatga aaaattcctg
     2281 ttggagcacc cttgcatgtt tatccggaga atttacatga aaaaaatact tcagggtcaa
     2341 aacatggcac gtccgtgtgc tatgtaactc gcccggaaga gaacatgaca tgcacaatga
     2401 aacaggatat tctgctacct tttttataat tttacaactg aaaaaacatt ggcgtagtgg
     2461 tgcataatca tggaaaacag gggatagatc tcagcaatgc cttcaaaatc ggcattatac
     2521 tgaatctggt cttcattgcc attgagatta tttatggatt ccttgcaaac tcgatggctc
     2581 ttgtagcgga tgccggacat aatttcagtg atgtgctggc cctggtcttt tcatggattg
     2641 cagtgaagct gtctgaacgc aaacccaccc tgaagtttac ctatggattc cggcgatcga
     2701 ccatactggt taccatactg aacacggtat tattgtttgt agccgtggga ttcctgttgt
     2761 gggaaactgt caggcggatc ggggagaaac aggttatcga ttcaaaactg gtgatgattg
     2821 tggcggctgc cggcattgtg ataaacggat ttaccgcatg gctgtttgtt gagggaaaga
     2881 agcatgatct caacattaaa agtgcatttc tgcatttcct tgccgacaca cttgtttcgg
     2941 ccggagtggt gcttgcaggc ctgattatgg ctctgacagg tatcaactgg attgactcgc
     3001 tgatgtcatt tataattatt gcaattattc tgtacaatgc ctacaagttg cttattgaat
     3061 ccgtaagcct tgccctggat gcagttcccg agaatattga cataagggaa gtcagcggat
     3121 atttttcttc cctgcccgaa gtaatcggaa tacatgatct gcatatctgg gctctcagca
     3181 caaattctgc cgctcttacc gttcacctgg aaaccaatat ccagactgat gccttttttg
     3241 ttcataccat aaggaagcat cttcatgaga aattcggaat tgagcatgct actattcagg
     3301 ttgagtacgg cggtcagccg ggtgagtgtt acaactgcaa ctgaagcctg tcttctctga
     3361 ttttgtattc tttgtttcag cagcagtgaa ccactgaatt atttttttct ccattcccat
     3421 ggcggaagag gatcaggtac tgcccacagg ccccttttcc tttttctggc aagtttttcg
     3481 gcaacaaggt attttttgtc ggatgaatac tgtttatatt gccaggcaaa tccctgtctg
     3541 acggataaaa gatttatatc ctgtttatta acatacaata caccaatcga cctgccatat
     3601 ctgtctgtgc cgcttatctg taccgaggcc tccctgttca ggtactgttt cagaaaatct
     3661 gccgactccg ccgcaaaagg ctgtttccgt tccggggcat ctattccgtg catcctgaca
     3721 ataagagaac cctcttctgt ctggaaaacg aatgtgtcac cgtcaataac cctgataaca
     3781 gaacccttat attcctgtga gaatccaaac ctgaaactga aactaacaat taacagcaga
     3841 aagatcttcc ggctcatata tcagtggatg aatcagtatg acgagccaaa aatagggatt
     3901 atttcatcaa aaccgggaaa caaattgatc tgagtaaact tgacgttgat aaactgaaag
     3961 aagaagattt aaacaggcat cttacaaaaa cattgaaatt gcgacttgag ggctttcatt
     4021 tattcatgtg ccgcaaaatg aaattattac ggtttaatac gacataattt tataatatta
     4081 gataaataac tatgtaaatc atatcatcct gatattccgt taataaacgt ttcaacaaaa
     4141 tccattatct ttttcagtat cctatcccca gttgtttttc tttgtaatag ggtcggttta
     4201 tcatatatta acagttccag tatctcgtct cttatcggtt cttgttcgga atacagataa
     4261 ttctcaataa atgcttgtgt tttttctttc gatagttttt cttcttcaac cattttcagg
     4321 aacgctcttt cctcttctat agcccaaaat ttctcaaacg cctgtggaat atcatcggta
     4381 tccagaataa cgggaaggtt ttctctgatg aacttttcaa tcagttcctt cttacttctt
     4441 aactgcattt ctgtattcaa caggttgaat atttcttttt caatttgctc tgcttctttc
     4501 ttctggtttg ctttcagttt gatgagcaga cgaataatgt atgcaacatt aatttcatca
     4561 cggtgaatga gttcgagttc aaaatcaaca tcctcaagta tgcttacctt ttcttttgca
     4621 tgttcactct ttactttatc atgcaggtca agatatttgc tcttataatc ctcgaattgc
     4681 tgctccgtca tggataaatc gctccacttg aaatcagaga aggatgtcag cacgttttta
     4741 attcgcatga gttccctgaa ggcaatgatg aatttcagtt catcatcttc agtgaccaga
     4801 ctgtttacgc tatctactgt tggtgttatc tgaagcagat tgtcaaaagc atcattaaat
     4861 gctttcacgt aatcatcata cggcttcatg atgatttctt cgatggcatc cttgttactg
     4921 aaaagggtta tggcttcatc ggtggcattt ttcagattac ggaagactac aatgtttccc
     4981 tgtgatttca gttcattcag gatgcggttg gtacgggaat atgcctgtat caatccatga
     5041 taccgcagac ttttatcaac ataaagggtg tttagcgacg gactgtcaaa tcctgtcagg
     5101 tacatgttta cgaccaacag tatgtcaatc tttcgctcct ttactttctt gctgatatca
     5161 ttgtaatagt tgtaaaaact ctgactgtcc cttgttgaaa atgctgaccc gaacatctgg
     5221 ttgtaatgct caataaattc atccagcttt tcacgggtat gcggattagt ggcatacgtt
     5281 atttcaggtt cggctgcagc ggaaatttct tctggaatga atccgtttgc atccacatca
     5341 gcttcgttgg caatatagct gaatatggtg gcaattttta aatcatgctc tccaagcatt
     5401 tttttgcgat acagcaactc atagtagcga atcagtgctg gaatactact gacgcagaac
     5461 atcgctgtaa actccctgtt atgcgttttc ctgctgtgat gggcaatgat atagtcagta
     5521 attttttcca gtcgttgagg agaatccatc agttcctcaa catctatatc ctcaacctgt
     5581 atgtcaatct cattggcact atccctgttc ttgtacctgc cgacatattc caccgagaat
     5641 ttcagcacat tttcatccct gatggcatcg gtaattacat atttgtgaag acaatcaccg
     5701 aataattcct ttgttgtttt cttgccgagt tcatttttta ctgcattttc agcaaagatg
     5761 ggtgtaccag taaaaccaaa catttggatg tttctgaaaa atgaaatgat acgctgatgt
     5821 gtttctccga actggctgcg gtgacactca tcgaatataa acacgatacg ttcatcctga
     5881 agtggcttca tccgctcctg aaagcgcttt ttactgatgg ctacattgag tttctgaatt
     5941 gtagttacaa tcagttttgt atcatcgcca aactgtctga ccagttgttt tgtattgtca
     6001 gtgccatcaa cgctcccttt gctgaatgaa ttaaattcat ttgtggtttg gtaatccaaa
     6061 tcctttcggt caacaacaaa cacaactttc ttaatctttg gcagtcgtgt taatatctgg
     6121 cttgctttaa atgaagtcac cgttttacct gaccctgtag tatgccagat atatccgttt
     6181 tttcggcaat gtagtaccct gtcaacaagt gcctcaactg cataatactg gtaggggcga
     6241 agcaccataa gtatcttgga tgtttcattg agtactatgt acttgcatat cattttggag
     6301 atgtgacatt tctccaaaaa atcagatgca aagtcgttga gcatattggt taaccgttta
     6361 ttttccttat cagtccagaa aaaagtctgc ttaaaagatt gaaagcggtt attggcgtaa
     6421 tatttcgtgt ttactccatt gctgattacg aatatctgga catattggaa caaaccatag
     6481 ctactgctga aagagtgtcg ctgatacctg tttacctgat taaatgcttc tttaagttcc
     6541 agtcctctac gttttagttc aatgtgcacc agaggaagac cattgataag taatgtaaca
     6601 tcataacggt tcttgtattt accttccata gagacctgat gggtaacctg atattggttc
     6661 tgacaccact gttcggtatt aatgaattca aaataaagat tatcgccatt atccctgatt
     6721 atatgctgtt tttcccgaag agtcttggct ttttcgaata ctgaaccctt acttacgata
     6781 tttaatactt tttcaaattc tttatcggag aatataatat tgttatgctt ttctaattgc
     6841 gatttcagat tggcaagtag ttccgattca tttgggatat ataccagaac atagcccagt
     6901 tcctgaagtt ttgcaatcag ttgttcttca agtattagtt cgggttgttt actcattgcc
     6961 aaagaggttc tttattccag ttgttcaaaa atcccaattt atatatcggt attgatggat
     7021 ttgattttat aagttcgtgt attttgctct taatctgatt gtttggagtt atcttgttac
     7081 acaaatagac catgcacgaa ataataagaa acggtttctt tacctgaaca ggaagaagtg
     7141 gattgacaaa ccatttcccg acaggattgt ttaatttttc gacaggtctt tttaccatat
     7201 tcctgctcca caaacgggag tgatgagcta taatattcct gacataggta atagcttcca
     7261 gccaactgga aagttcactg tgcaggttca atcccatttc ttttgcgatg gcggctttct
     7321 caggtaactg gtgtttcagg tttttataaa attttgatag cgtccccatt gaagccactt
     7381 ccaaggtctt ccatgcatct gcatccttgt ttgggtaacg aaagcgatgg tctttaatga
     7441 aactttcctg acttctgtca aattctttct tcaattcatc caaagtgttt tgatgaatgg
     7501 ttttaatcat accaccattt agcgtaatgg ttgtattctc gaagaatgcg gggtctaaat
     7561 accacaagcc accataactt acagacatgt gatatatcat ctttgtacgc agagcgattt
     7621 caatacgttc aatggcatcg aacagaaaaa gtctcaactg acggtcgaaa ttgtatctgt
     7681 caacaatgtc ttcaaaatat gtgtttggta aaaatgtatg caggttataa tctgattgca
     7741 tgt
//
LOCUS       DAOSPX010000140         7737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8872_length_7737_cov_3.810726, whole genome shotgun sequence.
ACCESSION   DAOSPX010000140 DAOSPX010000000
VERSION     DAOSPX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8872_length_7737_cov_3.810726"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2309
                     /locus_tag="PLZ75_08005"
     CDS             <1..2309
                     /locus_tag="PLZ75_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS72374.1"
                     /translation="AVSFRFSNSDNGIHDLHFHGESGELIGIMGGSGVGKSTTLSILN
                     GTLRPQSGEVRINGHDLYNHADREKLKGVIGFVPQDDLLMEELTVFQNIWFNARMCLN
                     NLSENQILETVNGILADFDLWEIKDLRVGNPLNKVISGGQRKRVNIALELLREPTILF
                     VDEPTSGLSSVDAEAVMNLLKEQTHKGRLVIVNIHQPGSEIYKMFDKIMIIDKGGYQV
                     YFGNPTEAIVYFKTITRHANPEEDQCVKCGNVDTDQILQIIEAKVVDEQGRATRIRKV
                     TPAEWAGQFRAIRKNRDREKIKEKGNLPVNNFSIPGRLKQSRIFFTRDLLSKLSDRQY
                     ILISLLGPPLLAFLLAYFTRYADGGIYRFSENDNITPYLFMCVITALFFGLMVSSEEI
                     VRDRKILRRESFLNLSWFSYLGSKVMLLFIISAVQTISFTLIGNLLLGIKGMTLSFWF
                     VLFTTSCFANILGLNISAALNSVVTIYIIIPFIIIPQILFSGVLVRFEKLHLSDFSSA
                     EYVPFVGDMMTARWSFEALAVTQFRDNAYERNYFETRAEVSRQEYDIMLINSLRNQLQ
                     QVMACSGRQEYRRDVENKLHKLAFHIDELSSSAAVVPGKWTEDLSPERFSPGTGKETA
                     AWLDSLKGIYSGLKKQSVRKENEISSMLGEKLGEEGLVRLREDNENKWLRNLLLSHDR
                     KELTEETDRRIIRKYEPAYMKATSKWGRAHFYAPVKRLLGLEIDTYWFNMAVIWTGSL
                     FFFILLYFNLIRRLLEYIENLRLPKVDS"
     gene            complement(2367..3872)
                     /gene="lysS"
                     /locus_tag="PLZ75_08010"
                     /pseudo
     CDS             complement(2367..3872)
                     /gene="lysS"
                     /locus_tag="PLZ75_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
     gene            complement(4004..4855)
                     /locus_tag="PLZ75_08015"
     CDS             complement(4004..4855)
                     /locus_tag="PLZ75_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOS72375.1"
                     /translation="METAFFNGNFIPKNEVKISPDDRGFLFADGIYEVIRWYTGFFYD
                     MEGHMSRLKRSLKEMRISWPESDSFPSVAAGLIERNDLKDKPSLVYLQVTRGTAVRTH
                     AFPVQPVPATVYASARSLSINPGMADRGVRVSVTEDPRWNRCDIKSVSLLPNILSLQQ
                     AVESGFSECVFVRNGFITECSHSNIFLAGGNVLYTHPESGYILSGITRKNVIRLAREA
                     GIDVRETPFPANRLNDITEAFITGTSFEITPVTAFDDFIVNRGRPGPLTELLRTKFRE
                     ELDRMIA"
     gene            complement(4935..6353)
                     /gene="fucK"
                     /locus_tag="PLZ75_08020"
     CDS             complement(4935..6353)
                     /gene="fucK"
                     /locus_tag="PLZ75_08020"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: protein motif:HMM:TIGR02628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HOS72376.1"
                     /translation="MTRGDIVIVFDCGATNARVIAIDGRGNILASESSPNSPVPDPFH
                     PGYRIWDVNEIWSAMCRASKKITAGISRNNIAGVTVTTFGVDGTLFDKAGNMLYPVIS
                     WQCERTAPVMNNIDKYIPLDVLYGESGVLPFTFNTINKLIWFSENRPELVGKSHRFLF
                     MPSIFILKLTGEMINDATMAGTSMLTSLSSRKFSPGILGSISFPPEKFGEIAEAGSIA
                     GQITPQASELTGIPVNVPVVATGHDTQFAIFGSGAEKNQPVLSSGTWEILMVRSESFK
                     TGREQLDFSITTEFDSIPGLYNIGNQWIASGILEWARKNLYADLTEDVYEKMIAGAEK
                     VPPGSKGVRVVPKFYEELKGKPGGQILGLTMESTRDEIYRAMLEALGLRLAEGKKALE
                     NAGGFRADSILCVGGGSKNRLWNRIRADYTGVPVKIIEQKETTVLGASLFVQAACGNA
                     SSPEEARSAIDYRTEIIEPGNI"
     gene            complement(6388..6798)
                     /locus_tag="PLZ75_08025"
     CDS             complement(6388..6798)
                     /locus_tag="PLZ75_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72377.1"
                     /translation="MDNSRDSRKILKEFNAIRKWALFLSVIGLIAAVSFAVTGLFTGI
                     FLSVFNVDDPAQGFPAWLSFLIIFLLFLLLLLPVIFLFRFSRFTSTAVRTNDEGIMHK
                     AVRNLKTHYLTLGILVIVILVLYFLFLILTMIST"
     gene            complement(6824..>7737)
                     /gene="fucI"
                     /locus_tag="PLZ75_08030"
     CDS             complement(6824..>7737)
                     /gene="fucI"
                     /locus_tag="PLZ75_08030"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991938.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOS72378.1"
                     /translation="EESRGHNAILAGFQGQRQWTDHMPNGDFMEAILCSSFDWNGIRP
                     PYLVATENDAMNGVPMLFGYLLTNTAQMFSDIRTYWSPEAIKRVTNISPEGLAANGII
                     HLINSGASSLDFSGRQTRDGESCIKPFWEITDKDARECLEATLWEPANLGYFRGGGFS
                     SQFNTKGVMPLTISRINLVKGLGPVLQLAEGWTTELPGEVHKILTDRTDPTWPTTWFT
                     PRLTGEGAFRDVYSVMANWGANHAASSYGHIGADIITLASILRIPVCMHNVPVEKIFR
                     PSAWNAFGMDKEGSDYRACQTYGALYR"
BASE COUNT         1896 a         1872 c         1909 g         2060 t
ORIGIN      
        1 acgccgtttc attcaggttc agcaacagcg acaacgggat tcatgatctt cacttccacg
       61 gagaatcggg cgaactgatc gggataatgg ggggaagcgg tgtgggaaag tcaacaaccc
      121 taagcatcct gaacggcacc ctcaggccgc agagcggcga ggtgagaatt aacggacatg
      181 atctctacaa tcatgctgac cgggaaaaac taaagggagt aatagggttt gtaccccagg
      241 atgatctgct gatggaggag ctgactgttt ttcagaatat ctggttcaat gccaggatgt
      301 gtctgaataa tctcagtgag aatcagattc tggaaacggt gaacgggatc cttgctgatt
      361 ttgatctctg ggaaataaag gatctcaggg tgggtaaccc cctgaacaag gttatcagcg
      421 gagggcaaag gaagagggtt aacatagccc ttgagcttct gagggaaccc accatactct
      481 tcgtggatga accaacttcc ggcctttcct cggttgatgc cgaggctgtt atgaacctgc
      541 tcaaggaaca gacgcacaag ggcagacttg ttatagtaaa tattcatcag cccggatcgg
      601 aaatatacaa gatgttcgac aagatcatga ttattgacaa aggcggctac caggtatatt
      661 tcggaaatcc aaccgaggca atagtctatt tcaagaccat caccaggcat gccaatcctg
      721 aagaggatca gtgtgttaaa tgcggcaatg tggataccga ccagatactt cagataattg
      781 aggcaaaggt ggttgatgag cagggcaggg ccacgaggat caggaaggtg acaccggctg
      841 aatgggccgg tcagttccgg gcaataagaa agaaccgtga cagggaaaag ataaaggaaa
      901 agggaaacct gcctgtaaat aatttcagca tacccggtcg gctgaaacag tcgcggattt
      961 tctttacacg tgatctgctt tcaaagctgt ccgacaggca gtatattctt ataagccttc
     1021 tcgggccgcc gcttctggcc ttcctgctgg cttatttcac caggtatgcc gatgggggga
     1081 tttacaggtt cagcgaaaat gacaatataa caccctatct cttcatgtgc gtcattaccg
     1141 cactgttttt cggactgatg gtcagttccg aggagatagt ccgcgaccgt aagatcctca
     1201 ggcgggaatc cttccttaac ctgagctggt tcagttatct gggctcaaag gtaatgctgc
     1261 tgttcatcat ctcggccgtt cagacaatct cctttaccct gatagggaac ctgctgctgg
     1321 gtataaaagg gatgaccctc tctttctggt tcgtgctgtt tacaacatcc tgctttgcaa
     1381 acatactcgg tcttaacatt tccgctgcat taaactcggt ggttacgatt tatatcatca
     1441 tacccttcat tatcattccc cagatcctgt tcagcggggt gctggtaaga tttgaaaaac
     1501 tgcatctttc cgacttttcc tcagccgaat atgttccctt tgtcggtgac atgatgactg
     1561 caagatggtc ttttgaagca ctggctgtga cccagttcag ggataatgca tatgaaagga
     1621 attatttcga gacccgggcc gaagtcagcc ggcaggaata cgatatcatg ctcatcaaca
     1681 gcctcaggaa tcagctccag caggtgatgg cttgcagcgg caggcaggaa taccggaggg
     1741 atgtggaaaa caagctgcac aagctcgctt tccacataga tgaattgtca tcttccgcag
     1801 ctgttgttcc cgggaagtgg acagaggatc tatcccccga aagattcagc cccggaaccg
     1861 gaaaagaaac ggctgcctgg cttgattccc tgaagggaat atattcaggc ctgaaaaaac
     1921 agtctgtgag aaaggaaaat gagataagca gtatgctcgg ggaaaaactg ggagaggaag
     1981 gtctggtcag actgcgtgag gacaatgaga acaaatggct caggaacctc ctgttgagcc
     2041 acgaccgcaa agaacttaca gaggagactg acaggagaat tatccggaaa tatgaacctg
     2101 catatatgaa ggcaacttcg aaatggggcc gggctcattt ttatgctccc gtaaaacggc
     2161 tgttgggact ggaaattgac acctattggt tcaatatggc agttatctgg acaggttcac
     2221 tgtttttctt tatactgctt tatttcaatc ttatcaggag actgctggag tatattgaaa
     2281 atctgagatt acccaaggtt gattcctgaa accggattcc cggtccccgg ctgctgatca
     2341 cctggttccg ggtgcctgtt tgctgattcc ggcttcatct gagggaacag gagcacatcc
     2401 tggattgagg gctggtttgt cagaagcatg gtcagccggt caatgcctat tcccattcct
     2461 gatgtgggag gcattccgta ttcaagcgcc ctgacaaaat cgagatctat gaacattgcc
     2521 tcctcatcgc ccttttctga aagcctgagc tgctccttga acctttcaag ctgatcgatc
     2581 gggtcgttaa gttcggaata ggcattggct atttcctttc cgtttattat aagttcaaac
     2641 ctctcggtga ggccgggttt actgcggtgc attttggtga gaggggaggt ctctatggga
     2701 taatcagtaa taaaggtcgg ctgtatgtaa tgctgttcac attttgcacc aaacagtaca
     2761 tcaatgagtt ttcccttgcc catgtcgggt gactggggca ctcccaggtg atcacatata
     2821 tccctgattt ccttttcatc catgcctgat atgtcgtatc cggtatgttc ctttatggaa
     2881 tcaagcatgc ttatgcgttt gtacggcgga ctgagatcta tggttttatc accgtaggca
     2941 atcaggcttt tcccgtgcag gtccatggct gccttactta ctattttctc tgtaaagtcc
     3001 atcatccagt tatagtcctt gtaggccacg tatatctcca ttaccgtgaa ttcagggttg
     3061 tgattcctgt ccattccctc gttgcggaag tccctggcaa attcataaac cccttcatat
     3121 cctccaacta tcagccgttt caggtaaagt tcattcgcga tcctcagata aaggggaata
     3181 tcgagagtat tgtgatgtgt tatgaaaggc cttgctgcag ctcctccggg gattggctga
     3241 agaacggggg tttcaacctc aaggtaaccc ctggaattga aaagctcccg catcgactga
     3301 acgatctgtg ttcttttccg gaaaacatcc ctcacagcgg gattgacgat caggtcgaca
     3361 tacctctggc ggtatctcat ttcaggatca gtaaccgcat cgtagtatat accgtccttt
     3421 tcctttacta tgggaagagg gcgcagggat ttggccagca gggtcagttc tctgacgtga
     3481 acagtgatct ctcccgtctg tgtggtgaat acaaaacccc tgatgcctat tatgtcgcca
     3541 atgtcaagca gccgtttaaa aacgatgttg tacagggtct tgtcctctcc cggacatata
     3601 tcatcgcgcc ttacgtaaat ctgtattctg ccggtggagt ccataagttc ggcgaaagag
     3661 gcattgccca ttatcctgcg gctcataatc cttcctgcca gggagacatc tccgaaacgc
     3721 ttttcctcag gcctgaagtt cccgcggata tcagcaataa aaatattcgt tctgtattcc
     3781 gcggcaggat aggggtcaat tcccagtttc ttcagttcat taagcgaact cctcctgatc
     3841 tgttgctgtt cactcagact taaatcattc atagcctaaa aaatttatgc aaatatatca
     3901 atcctgaacc attcaggaag aagccgggaa taataattaa gaagggttga gcacttaatc
     3961 ccggggaaca ccgtccggag cacggactca ctgaagggcc ttgtcaggca atcatccggt
     4021 ccagctcttc cctgaatttc gtgcgcagca actccgtgag cggtccgggc ctgccccggt
     4081 tcacaatgaa atcatcaaat gcggtaaccg gtgtaatttc aaatgaagtg cccgttatga
     4141 aggcctcggt gatatcgttg agacggtttg ccgggaaggg agtctccctc acatcaattc
     4201 ctgcctcacg ggccagacgg ataacatttt tccgggtaat acccgagagg atatagcccg
     4261 attcggggtg ggtgtaaaga acatttccgc cggcaagaaa aatgttggag tgggaacact
     4321 ccgtaataaa gccgttccgt acaaatacac attcgctgaa tccgctttcc actgcctgct
     4381 ggagagagag aatgttgggc agaagtgaca ctgatttgat gtcgcacctg ttccatctcg
     4441 gatcttcggt cactgacacc cttacgcccc tgtcagccat tccgggattt atgctgaggc
     4501 tgcgggcaga tgcatatact gttgcaggta ccggctgcac gggaaatgca tgtgtgcgta
     4561 ctgccgttcc gcgtgttacc tggagatata ccagtgacgg cttgtctttc agatcattcc
     4621 tttctatcag gccggccgct acggatggga atgaatcact ttcaggccag ctgatccgca
     4681 tctctttcag gcttcgtttc agtcgtgaca tatggccctc catgtcatag aagaatccgg
     4741 tataccatct tattacctca tatattccgt cagcgaaaag aaatccccgg tcgtcgggac
     4801 tgattttcac ttcatttttc ggaataaaat tcccgttgaa gaaagctgtt tccatatttt
     4861 ttcagtagtg atacgcagca gtgttgcatg gcagtccgca gaactgccat gggcaggtca
     4921 ggcgctgcaa aggttcagat atttcctggt tcaataattt cagtcctgta atcaattgcc
     4981 gatctggctt cttccggaga ggatgcattt ccgcatgcag cctgaacgaa aagtgatgct
     5041 cccagaacgg ttgtttcctt ttgttcaatt attttaaccg gaactcccgt gtaatcggcc
     5101 cttattctgt tccacagcct gttttttgaa ccgcctccga cgcacaggat gctgtcggcc
     5161 ctgaacccgc cggcattctc aagtgccttt ttcccttcgg ccagcctgag gccaagagct
     5221 tccagcattg cacggtaaat ctcatcccgg gtcgattcca tggtgagtcc aagtatctgt
     5281 ccgccgggtt ttcccttcag ttcctcgtaa aacttaggta caaccctgac tcccttactg
     5341 ccggggggta ctttttcagc tccggcaatc atcttttcat atacatcttc agtgagatcg
     5401 gcatagagat ttttcctggc ccattccagg atccccgatg caatccactg attgccgatg
     5461 ttatataatc ccggaatgga atcaaattcg gttgtgatgg agaagtcaag ctgttccctg
     5521 cctgttttga agctttccga tcttaccatc agtatttccc atgttcccga actgagcacg
     5581 ggctgattct tttccgcgcc cgatccgaaa atagcaaact gggtatcatg tccggttgca
     5641 acaacgggaa cattcacggg tatgcccgtc agttcagagg cctgcggggt gatttgtccg
     5701 gcaatgcttc ctgcttcggc gatttcaccg aatttttcgg ggggaaagct gatgctgccg
     5761 agaataccgg gggagaattt ccttgatgac agcgaggtca gcattgacgt cccagccatg
     5821 gtggcatcat taatcatttc tccggtgagc ttcagaataa atattgaagg catgaagaga
     5881 aacctgtgac tcttcccgac aagctccggc ctgttttcac tgaaccatat gagcttgttt
     5941 atggtgttga aggtaaatgg cagaaccccg ctttctccgt aaaggacgtc aagaggaatg
     6001 tatttgtcaa tattgttcat tacgggagca gtcctttcgc actgccatga tataacggga
     6061 taaagcatgt ttcccgcttt gtcgaacagg gtgccgtcaa ctccgaaggt ggtgaccgtt
     6121 actcctgcaa tattatttct gctgattccg gccgttatct tttttgatgc cctgcacatg
     6181 gcggaccata tttcattcac atcccatatt ctgtatccgg ggtggaaagg atccggaacg
     6241 gggctgttgg gagaggattc ggaggcaagg atgtttcccc tgccgtcaat ggcaattacc
     6301 cttgcatttg ttgcgccgca gtcaaatact attacaatat ctcccctggt catcagcagg
     6361 ctggtttttt caggtatgtt ttacaaatca tgttgaaatc attgtcagta taagaaagag
     6421 aaagtagagg accaaaataa ctattaccag tatccccaga gtcaggtaat gagtcttcag
     6481 gttcctgaca gctttatgca taatgccttc atcatttgtt ctgacggctg tggaggtgaa
     6541 tcttgaaaac cggaagagga atataaccgg aagcagcaac agcaggaaca acaggaagat
     6601 aattaaaaag gacagccatg cgggaaaacc ctgggccgga tcatccacgt tgaaaaccga
     6661 aaggaaaata ccggtaaaga gtcctgtaac ggcaaagctt acggcagcaa tcagaccaat
     6721 cacggaaagg aacaatgccc atttccgtat ggcattgaat tctttcagga tcttcctgct
     6781 gtccctgctg ttatccatgc cccgtcaaag agtatgaaat acattatctg tacagagccc
     6841 cgtatgtctg acatgccctg tagtccgatc cttccttgtc cattccgaat gcgttccagg
     6901 cgcttgggcg gaagatcttt tccacgggga cattatgcat gcataccggt atcctcagga
     6961 ttgatgccag cgtgataata tcggctccta tgtgtccgta gcttgatgcg gcatgatttg
     7021 caccccagtt ggccattacc gagtatacat ccctgaaggc tccttcgccg gtgagacgtg
     7081 gtgtgaacca ggttgtcggc catgtcggat ctgtcctgtc cgtcaggatc ttgtgaactt
     7141 ctccgggaag ctctgttgtc cacccttcgg ctaactgaag aaccggtccc agacctttga
     7201 ccaggtttat cctggagatg gttagcggca tgacaccttt tgtgttgaac tgcgaggaaa
     7261 atcctccgcc cctgaaatat cccaggtttg ccggttccca gagggttgcc tccaggcatt
     7321 cccttgcatc tttatctgtg atttcccaga agggcttgat acaggattcc ccgtcacggg
     7381 tctgtcgtcc ggagaaatcg agagatgagg cccctgagtt tatgagatgg atgattccgt
     7441 tggcagccag gccttccgga ctgatgtttg tcaccctctt aatggcttcc ggactccagt
     7501 aggtcctgat gtcagagaac atctgggcgg tattggtgag aagatatccg aaaagcattg
     7561 gcacaccatt cattgcatcg ttctccgtgg caacaagata cggtggtctt atcccgttcc
     7621 agtcgaagga tgagcagaga atggcttcca tgaagtcacc attgggcata tggtcggtcc
     7681 actggcgctg gccctgaaat ccggcaagaa ttgcattatg cccccttgac tcctcgc
//
LOCUS       DAOSPX010000141         7725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8891_length_7725_cov_3.960104, whole genome shotgun sequence.
ACCESSION   DAOSPX010000141 DAOSPX010000000
VERSION     DAOSPX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8891_length_7725_cov_3.960104"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="rsxC"
                     /locus_tag="PLZ75_08035"
     CDS             complement(<1..1196)
                     /gene="rsxC"
                     /locus_tag="PLZ75_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOS72379.1"
                     /translation="MKTFPLGGVHPPENKLTAGIPIRYLPIPPSVAIPVTQHIGSPSE
                     ILVKKGDMVKTGQIIAASKGFVSANIHSSVTGVVNKTDSVIDASGFRQTAVFIDTTED
                     EWLETIDRSSEIITEIKYSPEEIIDKCLESGIVGLGGATFPSHVKLSIPRGKRCDVLI
                     INGVECEPYLTSDHRLMLERSEEILVGITILMKALRVTQAMIGIENNKKDAIARLTGL
                     SGKFPGISIHGLKVKYPQGAEKQLIKALIKREVPSGRLPLDVNTVVHNVGTAFAVYEA
                     VQKNKPLFERVVTITGKSLASPGNYFVRTGTSVRYLIEAAGGVPENTGKIISGGPMMG
                     KAVSNADVPVVKGTSGIVLIPEEESSRSEMTPCIRCAKCVSVCPLNLEPYLLMTLSEK
                     GEFERAV"
     gene            complement(1235..2101)
                     /locus_tag="PLZ75_08040"
     CDS             complement(1235..2101)
                     /locus_tag="PLZ75_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOS72380.1"
                     /translation="MIIITILSLGVLALLAAIILYFIAQKFKVYEDPRIDEIQAILPG
                     ANCGGCGFAGCRAFAEALVRAESFDGLNCPPGGADVMAEAARLLGKEAPEVDPLVAVL
                     KCNGRPEYRPLTSFYDGVPDCRIAHSLYLGETDCSYGCLGKGDCVRACPFDAMYMNSE
                     TGLPEIIDDKCVACGNCVKACPRNLIELRKKARKDRKIYVACSNCDRGGPARRACRVA
                     CIACMKCVKVCKFDAITVENYLAYIDSFKCTLCRKCVAECPTGSILEFNFPPRKPAAE
                     VTSQTIKVNPEV"
     gene            complement(2110..2535)
                     /locus_tag="PLZ75_08045"
     CDS             complement(2110..2535)
                     /locus_tag="PLZ75_08045"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOS72381.1"
                     /translation="MNRSNSYDNIEHEGTVQRSDSNLVIVSISSEAACSACHAKGACS
                     LSGAKEKLIEIPGRYDLVPGDNVTVVMHRSAGFRAVLFAYVLPFILLIPALLTLSSLG
                     ISEPAAALGSVAVVSIYYLVLFFFRDRIANNFTFSIKTP"
     gene            complement(2650..4755)
                     /gene="recG"
                     /locus_tag="PLZ75_08050"
     CDS             complement(2650..4755)
                     /gene="recG"
                     /locus_tag="PLZ75_08050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOS72382.1"
                     /translation="MQDLLETNITYLPGVGPRRAELLNSELNISTYRDLLYYFPYKYI
                     DRTRFYRIAELEPDLPHVQIRGHITGFYSEGGGRGKRLVADFSDDTGTIRLIWFRGAR
                     WITGTYGPGPEYIVFGKPGVFNGQINIIHPEIEAAEKTAERITSALQAHYSTTEKLKN
                     HFLSSKAISKLIATLLQKINFTLPETLPGYIIGKYNLLELGKALYSIHFPQDPGILEK
                     ARYRLKFEELFYIQLNLIRFKSNRSRKYRGFVFSNVGEKFNTFYFRNLPFELTSAQKR
                     VMKEIRKDLGSGIQMNRLLQGDVGSGKTLVALMSMLIAVDNGYQACIMAPTEILAKQH
                     YQTIVSLLRDMDVSVKLLTGSSAGSLRKEISRSLLDKSLNILIGTHALIEDNVQFGNL
                     GLVIIDEQHRFGVEQRARLWRKSSNPPHVLVMTATPIPRTLAMTLYGDLDVSVIDELP
                     PGRIPVRTMHFFDSEKNKVYGFMKKKIEEGSQVFVVYPLIRESEKMDYRDLEDGWDTI
                     SRLFPPPRYCISVVHGQMTPAIKEESMKLFREGKAHILIATTVIEVGVDIPNASVMVI
                     ESAERFGLSQLHQLRGRVGRGSDQAYCILMSGDKLSKEASARIDVMTRSNDGFEIAEQ
                     DLLLRGPGDIEGTMQSGIPFDLRIADLGKDGQLIAWVRNIAEEIIESDPLLEKDSNAI
                     LKHMLKKMFSKKQSWSNIS"
     gene            4980..5768
                     /locus_tag="PLZ75_08055"
     CDS             4980..5768
                     /locus_tag="PLZ75_08055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS72383.1"
                     /translation="MLSVRAVKYLKYILFSRHRKGFGIHSPFVFDLVSRVFRNKTDPG
                     IVLLVENIRKRQISDKTRIEVLDLGSGSLALKGSLKRVCDITKYSAVPPAYGKLLYRM
                     AQEFGGPGIVEFGTSTGISTMYLAAASPDAQVITLEGCPALSALAARNFSSIGLANIV
                     QIKGDFDDSLRELRKRSVQPGLVFIDGNHRREATVRYFREMADMSGDETVIIVDDIHL
                     SAEMEEAWKEILNSGNVSVSIDIFRMGMVFFRKGMSRFDYVIRY"
     gene            5842..6543
                     /locus_tag="PLZ75_08060"
     CDS             5842..6543
                     /locus_tag="PLZ75_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS72384.1"
                     /translation="MDKVIEIQNIVKNYQVGSVIVRALRSVSININRNEYVAIMGPSG
                     SGKSTLMNLLGCLDTPTSGTYILNGIDVSKMEDDYLAEIRNKEIGFVFQTFNLLPRYT
                     ALENVTLPLIYAGVPKAEREKMAVETLTQVGLADRMTHKPNELSGGQRQRVAIARALV
                     NNPSIILADEPTGNLDSKTSLDIMGLLDIIHKSGNTVIVVTHEEDIARHAARIVRLLD
                     GEIFQDVLNENYKPA"
     gene            6561..7103
                     /locus_tag="PLZ75_08065"
     CDS             6561..7103
                     /locus_tag="PLZ75_08065"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOS72385.1"
                     /translation="MKIYTRTGDDGTTSLAGGSRIPKHHARIEALGSIDELISWLGLL
                     RDLKENSGHKDFLIHLQSQLMKFAAVISSGDDRKVAEKLLPDEESVKAIEDEIDKMAK
                     DLIPLEYFIIPGGNVIVSYCHIARCVCRRAERKVLKLYSKNKIIPLAGVFLNRLSDYL
                     FILSRKISSDLDIEETNWLI"
     gene            7198..7419
                     /locus_tag="PLZ75_08070"
     CDS             7198..7419
                     /locus_tag="PLZ75_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOS72386.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDFAIRSGAPLEVVENLQEIE
                     DEGDIYEGIEDIWPDYPSKDDFFFNEDEY"
     gene            complement(7502..>7725)
                     /locus_tag="PLZ75_08075"
     CDS             complement(7502..>7725)
                     /locus_tag="PLZ75_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72387.1"
                     /translation="LGEGDEIMAVNKATSSEIEIYIDNGKISVITENQSPEGVIDPPS
                     EAPQGNPHLEGFRWYDHLRPKKILDIFKH"
BASE COUNT         1992 a         1759 c         1761 g         2213 t
ORIGIN      
        1 acggctcttt caaattctcc cttttccgaa agggtcatga ggagataagg ttcaaggtta
       61 agaggacaaa cgctgacaca ttttgcgcaa cgtatgcagg gagtcatctc cgatcttgac
      121 gattcttctt ccgggataag cacaatgccc gatgttccct ttaccacggg cacatccgca
      181 ttgctcactg cttttcccat catgggacct cccgatatga ttttcccggt attttcagga
      241 acacctcctg cagcttcgat aagatacctt acggaagttc ctgtcctgac gaagtaattt
      301 ccgggactgg caagcgattt gcctgtgatg gtgaccactc tttcaaacag cggcttgttt
      361 ttctgaacag cttcgtacac cgcaaaagca gttccgacat tatgaaccac cgtatttaca
      421 tcgagtggca gcctgcctga ggggacttcc ctctttatga gtgctttgat cagctgcttt
      481 tctgccccct ggggatattt taccttaagt ccgtgtatgc tgatgccggg aaatttcccg
      541 gacagcccgg tgagacgggc tattgcatcc tttttgttgt tttcaatgcc aatcatggcc
      601 tgtgtcaccc tgagtgcctt catcagaata gttattccga caaggatttc ctcgcttctt
      661 tccagcatca gcctgtggtc ggaggtaagg taaggctcac attcaactcc gttgattatc
      721 agaacatcac atcttttacc ccggggaatg ctgagcttaa catgtgaagg gaaggttgct
      781 ccgccaagcc cgacaattcc tgattcaaga cacttgtcaa taatctcttc gggtgaatat
      841 tttatttcag tgattatttc actgctcctg tcaatagttt caagccactc atcctccgtg
      901 gtatcaataa atacggcagt ctgcctgaat cccgatgcat caatcacgct gtctgtttta
      961 tttacaactc ctgttaccga tgaatggata ttggctgaaa caaatccttt gctggcggca
     1021 attatctgtc cggttttaac catgtcgcct ttttttacga ggatttctga gggagagcca
     1081 atatgctgtg taacgggtat tgctacggaa ggaggaattg gcagatatct gatcggtatt
     1141 ccggctgtca gcttattttc gggcgggtga acaccaccca gtggaaacgt tttcaagttt
     1201 gctcttaatt attatgttat actgcattat tatgttatac ttccggattt acttttatgg
     1261 tttgactggt gacctcagcg gccggtttcc ttggggggaa attgaattca agtatggaac
     1321 cggtcggaca ctcggcaaca catttcctgc agagagtgca tttaaatgaa tctatgtagg
     1381 caagataatt ctctacggtt attgcatcga atttgcagac ctttacacat ttcatacagg
     1441 caatacaggc taccctgcat gccctgcgtg ccggaccgcc cctgtcgcag ttggaacatg
     1501 caacatagat tttacggtcc ttcctggctt tcttcctcag ttcaatgagg tttctcgggc
     1561 aggctttcac gcagttgcca caagcgacac atttatcatc aattatctca ggcaggcctg
     1621 tttcgctgtt catatacatg gcatcgaatg ggcaggccct tacacagtcg ccttttccaa
     1681 ggcagccata actgcagtcc gtttcgccca ggtacagact gtgggcaata cggcaatcag
     1741 gcactccgtc atagaatgag gtaagcggcc ggtattcggg tctgccgttg catttcagaa
     1801 ctgcaacaag aggatccact tcgggagctt cctttcccag cagtctggca gcttcagcca
     1861 taacgtcagc tcctcccggg gggcagttaa ggccgtcaaa ggattctgcc cttacgagtg
     1921 cttcggcaaa ggcccggcag ccggcaaaac cgcatccccc gcagttggca ccgggcagaa
     1981 tagcctgtat ctcatctatg cgcggatctt catacacctt gaatttctgt gcaatgaagt
     2041 aaaggattat tgctgcaagc agtgcgagca cacccagact taagattgtt attataatta
     2101 tgtgactcat taaggagttt ttattgaaaa cgtgaaattg tttgctattc tgtcacggaa
     2161 gaaaaacaga acaaggtaat agatgctgac cacggcgaca gaacccagtg ctgccgcagg
     2221 ttccgatatt ccgagtgaac tgagtgtcag gagggccggg atcagtaaaa tgaagggaag
     2281 gacataggcg aacaataccg ccctgaatcc tgctgatctg tgcataacaa ccgtaacgtt
     2341 gtcacccggc accagatcat accggcccgg aatctcaatc agtttttcct ttgcccccga
     2401 caggctgcat gcaccttttg catggcaggc cgagcatgcc gcttcagatg atatactgac
     2461 tattacaagg ttgctgtccg atctctgaac tgttccctca tgctctatat tatcgtaact
     2521 gtttgatctg ttcataaatt tctccggaaa gaaaatccca aatttactaa taaggataaa
     2581 ttacttactg ctttttatca tgaaattggg ttctcaggat aatttattcc ggttttaaat
     2641 agtttttcat cagcttatat tactccatga ttgtttcttg ctgaacattt ttttcagcat
     2701 atgtttcaga attgcattac tgtctttttc cagcaggggg tcagactcta tgatttcttc
     2761 cgcaatatta cgtacccagg caatcagctg accatccttc ccaaggtccg cgattctgag
     2821 atcaaaggga attccgcttt gcatggttcc ttcaatatca cccgggcctc tcagcagaag
     2881 atcctgctcg gcaatttcaa aaccatcgtt tgatcttgtc attacatcta ttcttgcaga
     2941 agcttccttt gaaagtttat ctccgctcat cagaatgcag tatgcctggt ctgatcccct
     3001 gccgacacgt ccccgcagct ggtgaagctg ggaaaggccg aatctttccg cactttcaat
     3061 gaccatcacc gatgcattgg gaatatcgac tccaacctct atcactgttg ttgctatcag
     3121 gatatgagcc tttccctccc tgaacagttt catcgactcc tcctttatgg cgggggtcat
     3181 ctgtccgtga accacgctga tgcaatagcg gggaggcgga aataatcttg aaatagtgtc
     3241 ccagccatct tcaagatccc tgtagtccat tttttccgat tccctgataa gggggtaaac
     3301 cacaaacacc tggcttcctt cctcaatttt ctttttcatg aatccataca ccttattctt
     3361 ttcggaatca aagaagtgca tggttctgac cgggatcctg ccgggaggca gttcatctat
     3421 caccgacaca tccaggtcac cgtacagggt cattgccagt gttctgggta tgggagttgc
     3481 cgtcattacc agaacatgag gcggattgct gctttttctc cacagcctgg ctctctgttc
     3541 aacgccaaac ctgtgctgtt catcaatgat taccagaccc agattgccga actgcacatt
     3601 atcctcaata agagcatgtg tgccgataag aatattcagt gatttatcca gaagcgaccg
     3661 gcttatttcc ttcctgagtg atcctgcaga tgaaccggtg agaagcttta cgctcacatc
     3721 catatcacgg agcagggaaa cgattgtctg gtaatgttgt tttgcaagta tctcggtggg
     3781 tgccattatg catgcctgat agccgttgtc aaccgctatc agcatactca tcagggcaac
     3841 gagggtcttt ccgcttccca catcaccctg aagcagcctg ttcatctgaa tcccggatcc
     3901 gagatccttt ctgatctctt tcatcacccg cttttgtgcg gaagtaagtt caaaggggag
     3961 gttcctgaaa taaaatgtat tgaatttttc ccccacgttt gaaaatacaa agcctctgta
     4021 cttccgggaa cggttgcttt taaaccttat gagattcagc tggatgtaaa aaagctcctc
     4081 gaacttcagc ctgtatcttg ccttttccag tattcccgga tcctggggaa aatgaattga
     4141 atacagggcc ttgcccagtt caagaagatt gtatttcccg atgatatatc ccggcaatgt
     4201 ttcgggcagc gtgaagttga tcttctgtaa caatgtagca atgagcttac tgatggcttt
     4261 ggatgaaagg aaatgattct ttaatttttc ggtagtgctg tagtgggcct gcagtgccga
     4321 tgtaatcctt tctgctgttt tttccgctgc ctcaatctcg ggatgtatta tgtttatctg
     4381 cccgttgaag acgccaggtt ttccaaagac aatatactcc ggccccggac catatgttcc
     4441 ggtgatccat ctggctcccc tgaaccaaat aagccttatt gtacccgtat cgtcgctgaa
     4501 atcggccaca agcctttttc cccgtcctcc gccttccgaa tagaatccgg taatgtgacc
     4561 cctgatctga acatggggca gatcaggttc cagttcagcg atcctgtaaa aacgcgtcct
     4621 gtcaatatat ttatatggaa aataatacag gaggtcacgg taagtgctga tattgagctc
     4681 gctgttcaga agttcggccc ttctgggccc cacacccgga agatatgtta tatttgtttc
     4741 gaggagatcc tgcattccgg caatgttacc catactttca atgattgacg aaattaagta
     4801 tttttggaga gcttacctaa tccggctgac catggggttt gtacaagcat tcaggggaac
     4861 cacggtcttc aggaggtttt ttaggaagtc caggtattgc agaaatctgg tgaaaatcaa
     4921 ggctttgcag aggttttgtg taaatcccga cctcgcggga atgatattta aatctgacta
     4981 tgttatccgt aagggcagta aaatacctga agtatattct cttttcaagg cacaggaagg
     5041 gttttggaat acacagtccc tttgtttttg accttgtttc gcgtgttttc aggaataaaa
     5101 ctgatcccgg gattgtctta ttggttgaaa acatcagaaa aaggcagatt tccgacaaga
     5161 caagaattga ggtccttgat ctggggtccg ggtctttagc attaaaagga agtttaaaaa
     5221 gagtgtgtga cattacaaaa tattcggctg ttccgcccgc atacggaaaa ttgctttaca
     5281 ggatggcgca ggaattcgga ggaccgggaa tagtagaatt cggcacatcc accggcataa
     5341 gtacgatgta tcttgcagcg gccagtccgg atgcgcaggt tatcaccctg gaaggctgcc
     5401 cggccctgtc agctcttgcc gcacgtaatt tcagcagtat aggactggcc aatattgttc
     5461 agataaaagg tgattttgac gattcactca gggaattaag aaaaagatcc gtacagcccg
     5521 gactcgtatt cattgacggg aatcacagga gggaggcaac agtgaggtat ttccgtgaga
     5581 tggcagacat gtcgggagat gaaacagtaa tcattgttga tgacatacac ttgtcggctg
     5641 aaatggagga ggcatggaaa gagatactga attcagggaa tgtttcagtc agcatcgata
     5701 ttttcaggat gggaatggtt tttttcagaa agggaatgag ccgttttgac tatgtgatac
     5761 gctattaata aaatttaata cacattaatt cagaattaag ttaaaataat ctaatattgc
     5821 aaattctaat acagcaggtt tatggataaa gttattgaga ttcagaatat tgttaagaac
     5881 tatcaggttg gttcggtaat agtcagggct ctgaggtcag tgtcaataaa tataaacagg
     5941 aatgaatacg ttgccataat gggtccctcg ggctcgggta agtcaaccct gatgaacctt
     6001 cttggctgcc tggatactcc caccagcgga acctacattc tcaacggcat agatgtcagc
     6061 aaaatggagg atgattacct ggcagagatc agaaacaagg aaattggctt tgtcttccag
     6121 acctttaacc tgctgcccag gtatacagct cttgaaaatg taactttgcc attgatttat
     6181 gccggtgtgc ctaaggctga aagggaaaag atggcagtag aaaccctgac ccaggtcgga
     6241 cttgctgaca ggatgacaca caagcccaat gagctgtcgg gggggcagag gcagcgtgtt
     6301 gccattgcac gggccctggt aaacaatcct tcaatcatac tggctgacga gcccactgga
     6361 aacctcgaca gcaaaacatc cctcgacatc atggggctgc ttgatataat ccacaagagc
     6421 ggcaatacag tcattgtggt tacccacgaa gaagatattg cacggcatgc cgcgaggata
     6481 gtgagacttc tggacggaga gattttccag gatgttctta acgagaatta taaacccgcc
     6541 tgattataat tttccggcct atgaagattt acacacgaac cggtgatgat ggaacgacat
     6601 cgcttgccgg aggcagcaga attcccaagc atcatgccag gattgaagcc ctgggcagca
     6661 ttgatgaact gatatcatgg ctcggactac tcagggatct gaaagagaat tccgggcata
     6721 aggattttct tattcatctt cagagccagc ttatgaaatt tgctgcagtg atttcttccg
     6781 gggatgaccg gaaggttgcg gagaaacttc ttccggacga ggaatctgtt aaagctattg
     6841 aggatgagat agataaaatg gcaaaagatt tgattcccct ggaatatttc atcattcccg
     6901 gcggtaatgt tattgtatct tattgtcata tagcaaggtg tgtctgccgc agggcagaaa
     6961 ggaaagttct gaaactctat tcaaaaaata aaataatccc tcttgcaggc gtatttctaa
     7021 accgtttgtc cgattatctt ttcattttgt ccagaaaaat cagttcagat cttgatattg
     7081 aggaaactaa ttggctgata taatgtgagt aaatattgca tggtttatat tgtttttcag
     7141 ttaaaaaaat atatatattt gcaaactttt ttaaaaactc acccttaaag gaatattatg
     7201 tactggacac ttgaattagc atcaaaactt gaggatgctc cctggcccgc caccaaggat
     7261 gaacttatag attttgctat aagatcgggt gcccctctgg aggttgttga aaacttacag
     7321 gaaatcgagg atgaggggga tatatatgaa ggtattgagg atatctggcc tgattatccc
     7381 agcaaagatg actttttttt caatgaagac gaatattagg ggaggatcat ttagttatag
     7441 gtagagctgg tttttccgtc ccggtccttc tctctgccat attaaatgtt gagcactgta
     7501 ttcagtgctt gaagatatcc agaatcttct ttggtcgcaa atggtcatac catctgaatc
     7561 cctcaaggtg cggattgccc tgtggtgctt cggacggagg gtcaatcacc ccttccgggc
     7621 tctggttctc cgtaataact gaaatcttcc cgttgtcaat gtaaatttct atttcggaac
     7681 tggtagcctt gttgacagcc attatttcat ccccttctcc caggt
//
LOCUS       DAOSPX010000142         7711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8914_length_7711_cov_3.995298, whole genome shotgun sequence.
ACCESSION   DAOSPX010000142 DAOSPX010000000
VERSION     DAOSPX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8914_length_7711_cov_3.995298"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PLZ75_08080"
     CDS             <1..898
                     /locus_tag="PLZ75_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72388.1"
                     /translation="VHRAPAVAAMEAGAHVLVEKPFASSLEDCDAMLSAAERYDRQTG
                     VISQRRWYRPVQRVKEAIDSGKIGKPVFGTVNMLGWRDEKYYQSDPWRGSWKGEGGGV
                     LVNQAPHQLDLLQWFMGGIDELYGIWSNLNHPYIEVEDTALAIIRFRNGAIGNIVVSN
                     SMKPGIYGKVHVHGQNGASVGVQTDGGAMFVAGMSDILEPPVNDLWTVPGEENLLENY
                     VREDSEFFLSLKNPVEYFHERQIEDFLAAIKEGREPLVTGREGRVTVEIFTAIYRSAR
                     DGRPVKWPLYPENRNDYDGRPV"
     gene            1039..2115
                     /locus_tag="PLZ75_08085"
     CDS             1039..2115
                     /locus_tag="PLZ75_08085"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS72389.1"
                     /translation="MKLKAILFLMLAAVIYGCQERYYTEDDFRSVLKIDSHIHINSGD
                     GVFEEQAADDNFILITLNVDNGNLSDIRDQFNYAVLSAQKHPGRVFFGPTFFFDTAGW
                     GTETWSRKVVDQLDSDISAGVITVKIWKNIGMTVRDRSGKFIMVDDPRLDPVINLIKS
                     RNIPVTGHLGEPLNCWLPLEEMTVRNDRNYFAQHPRYHMYLHPEYPSYEDQINARDNF
                     LERHPDLPFIGCHLGSLEWNVDSLALRLDRYPNMAVDISARTCHLQYQSARDRERVRD
                     FCIKYQDRLLYGTDIGYMGGGNPEAFKTRVHDTWIDDWKYFATGSEMTSPLFEGGFTG
                     LQLPKKVIDKLYRENAVRWYKLKI"
     gene            2150..3592
                     /locus_tag="PLZ75_08090"
     CDS             2150..3592
                     /locus_tag="PLZ75_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOS72390.1"
                     /translation="MITFVISVTALLLGYFLYSKFLEKIEGADPDRETPAFKMKDGVD
                     YVPLPCWKTFLIQLLNIAGLGPIFGAIAGAMWGPVAFLWIVLGTIFAGGVHDYFSGML
                     SMRYNGLSITEITGMFLGKGVKQFMRAFTVLLMVLVGAVFIMGPAGILAGLTPAHMTM
                     TFWVWVVFGYYIIATLLPIDKIIGRLYPVFGFALLFMAAGLITALFVKGYHIPELTLS
                     NFRNFHVNPDKFPVFPMMFISIACGAISGFHSTQSPLMARCITNEMLGRRVFYGAMVS
                     EGIIALIWAAIGMSFYGGVGALNEMMTAQKGNAAYLVNEISNSLLGRFGGLLAILGVV
                     AAPITSGDTAFRSARLIVADFLKIRQGPLLNRLYISVPLFLVGFLITRMDFAVIWRYF
                     AWSNQTLAMVVLWTITVYLLQTGRLYMVTLIPAFFMTAVCVSYILFAPEGFSLPGAIS
                     HTAGIIAAVIVLTVFLVYKQKIEKTAPGFG"
     gene            3732..4487
                     /locus_tag="PLZ75_08095"
     CDS             3732..4487
                     /locus_tag="PLZ75_08095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOS72391.1"
                     /translation="METDNSRRGFLRKAAFGSLAAISIPEIVSAALPQERNGIKLLKG
                     QTILFQGDSITDAGRSRKETGFNNAGALGSGYPMLAAARLLLSHAGLDLKIYNRGISG
                     NKVFQLAERWDEDCIDIKPDVVSILIGVNDIWHKLGGNYNGTVEIYRRDYIALIERTK
                     KALPAVKLIIGEPFGVRGVKAVDDSWYPEFYEYQKAAKEIASQFGAQFIPYQAVYDEA
                     QLRAPAAYWTGDGVHASLAGASLMAEAWLRMVE"
     gene            4654..5910
                     /locus_tag="PLZ75_08100"
     CDS             4654..5910
                     /locus_tag="PLZ75_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS72392.1"
                     /translation="MIKTETAVKNASGNFPGILLILFFIFLPGIIFGQETVYGLLYSD
                     NKPVSVTFRDGVITEVRKIKKLPSGSEGLIIGPGLIDNQINGFAGVSFTFGGGELTRD
                     GVIKATEAIWKTGVTTYLPTLTTNSHDVLMKNFAVLGSIAGDAALLGSIPGFHLEGPY
                     ISPVDGYRGAHPLDHVRKPDWQEFLQYRKAAGNKIIHVTIAPEVEGAMDFIDKCRAEG
                     IVVALGHHNGSAKEITEAIDRGAIIATHLGNGCANMINRHLNPLWPQLSDDRLMISII
                     GDGFHLNPEEIRVFYKVKGPDKTIITSDVTSYASLEPGRYLTDEGEAIELTPEGMLKY
                     PAQDVLYGSASPVKKGLVNVMKYTGCSLEEAFRMAALNPAKLYGLNDRGEIARGKRAD
                     IILFRIENDEMIIKKTWVKGKLVYQE"
     gene            6310..>7711
                     /locus_tag="PLZ75_08105"
     CDS             6310..>7711
                     /locus_tag="PLZ75_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS72393.1"
                     /translation="MKKLRIMALLFLFLASWGIASAGSAVPGNGDINAADMQQITVTG
                     TVTDASTREPMPGVNVLVSGTTLGALTDLSGRYSLNVPGRDAVLVFSFIGFITQEQAV
                     AGRTVINVALEPATEALDEVIVIGYGTQKKSDLTGSVSRVTFDDKATLANLNLSQAMS
                     GSTAGVNIQQGSGLAGAEPSLDIRGQTSLSASDRPLIVLDGIIFNGSISDINVNDVEA
                     VDILKDASAAAVYGSRSANGVILITTKRGKSEKPLIGFNMYYGYQNMSNNPMRVMNAE
                     EYAIRLVDYYYQQDLYTWYKTKPTSSEGKPVRPDITNRELVAAKLRTAEERDNYIAGN
                     EIDWVKEVMRTAPIQNYNLSLSGKSDKSNYFLSASYSDEKGILRNDQFNRITLHSNVE
                     SKITDWLTVGLISSYSYRDYSGINASLGDARSCSPLANNKIGRPDYDMFLTGEAYMTY
                     PLVYEYIKNKDVRNNLF"
BASE COUNT         2113 a         1673 c         1958 g         1967 t
ORIGIN      
        1 ggtccacagg gctccggctg ttgctgccat ggaggcgggg gcccatgttc tggttgaaaa
       61 gccattcgct tcatccctgg aagattgtga tgcaatgctc tctgcagctg aaagatatga
      121 cagacagacc ggagtcataa gtcagcggcg ctggtacagg ccggttcaaa gggtgaagga
      181 ggcgattgat tcgggaaaga taggaaaacc tgttttcggg actgtcaaca tgcttggctg
      241 gcgggatgaa aagtattatc agagtgatcc ctggaggggc tcatggaagg gtgagggagg
      301 cggcgtcctc gtgaaccagg ctccccacca gctcgacctt ctgcaatggt tcatgggcgg
      361 gatagatgaa ctttacggta tctggtcaaa tctgaaccat ccatacatag aagttgaaga
      421 tacggccctg gcgattatca ggttcagaaa cggggccatc ggcaatattg tcgtatcaaa
      481 ttcaatgaaa cccggaattt acgggaaggt gcatgtccac gggcagaacg gggcatctgt
      541 cggagtgcag accgacggag gtgcaatgtt cgtcgcggga atgtcagaca tcctcgagcc
      601 tccggttaat gatctctgga ccgttcccgg ggaagaaaac ctgcttgaaa attatgtaag
      661 ggaagattcg gaattttttc tttcactgaa aaatccggtg gagtatttcc atgagaggca
      721 gatagaggat tttctggcag ccataaaaga gggacgcgaa ccgcttgtta cgggaaggga
      781 aggcagggta acggttgaga tttttactgc catttaccgc tcggcccggg acggcagacc
      841 tgtgaaatgg ccgctttatc cggaaaaccg gaatgattat gacggcagac cggtttaaat
      901 cccggtttat ggggaaaatt taaaataatt ctttttaaat taactgcgat ttttcagccc
      961 ctgtgaatgt aatcatccgg accggggcgg gaaatcatat tataaattat ggtcatatca
     1021 acaaaaacca gacagaaaat gaaactgaaa gcaattttgt tcctgatgct ggcagcagtg
     1081 atttacggtt gtcaggaaag atattatact gaggacgatt tccggtcggt actcaaaatc
     1141 gattcccata tccatattaa tagcggtgat ggtgtttttg aagaacaggc tgcggatgac
     1201 aatttcattc tgataacact taatgttgac aacggtaacc tgtctgacat cagggatcag
     1261 ttcaactatg cagttctttc tgcacaaaag catcccggca gagtcttttt cggacccaca
     1321 tttttcttcg acacggctgg ctggggtact gaaacctgga gcaggaaggt agttgatcag
     1381 cttgattctg atatttcagc aggcgtcatt accgtcaaga tctggaaaaa tatcggcatg
     1441 acagtccgtg acagatcagg aaaattcatt atggttgatg atccccgact ggatcctgtt
     1501 ataaatctca taaaaagcag gaatattccg gtaaccgggc atctcggaga acctctcaac
     1561 tgctggcttc cccttgagga aatgactgtc agaaacgaca gaaactattt tgcccagcac
     1621 ccccggtacc atatgtacct tcacccggaa tacccgtcct atgaagatca gataaatgcc
     1681 cgtgataatt tcctggagag acatcctgac cttcccttta tcggctgcca tctcggaagc
     1741 ctggaatgga acgttgattc ccttgccctc aggctcgaca ggtatcccaa catggctgta
     1801 gacatatccg ccaggacatg ccatctgcag tatcagtctg cccgcgacag ggaacgggtt
     1861 cgtgatttct gcataaaata ccaggacagg ctgctttacg gaactgatat aggttatatg
     1921 ggaggtggca atccggaggc cttcaagaca cgggtgcatg atacatggat tgacgactgg
     1981 aagtattttg ccaccggctc ggaaatgacc tcccccttgt ttgaaggcgg atttacaggt
     2041 ttgcagcttc cgaaaaaagt gatagacaag ctgtaccgtg aaaatgccgt aagatggtat
     2101 aaactgaaaa tataaaatac tgatatcctg aaataaatag tttctacagt tgatcacgtt
     2161 tgtcatttca gttacggccc ttttgctggg atatttcctg tattcgaaat ttctggagaa
     2221 aattgaagga gccgatccgg accgcgaaac ccccgccttt aaaatgaaag acggtgtgga
     2281 ttatgtgccg cttccgtgct ggaagacttt tctgattcag ctgcttaata ttgccggact
     2341 gggaccgatc ttcggtgcta ttgcgggtgc catgtgggga cccgtagcct ttctctggat
     2401 agtgctggga acgatctttg caggtggtgt tcatgactac ttctcaggaa tgctgtcgat
     2461 gcgctataac ggattgagta ttactgaaat aaccggaatg ttcctgggga agggagtgaa
     2521 gcagttcatg agggccttta ccgttttgct gatggtactg gtcggggctg tttttattat
     2581 gggaccggcc ggaatacttg caggactgac tcctgcacat atgacgatga cattctgggt
     2641 ttgggttgtt ttcggatatt atatcattgc aacccttctg cccatcgaca agatcatagg
     2701 gagattatat cctgtatttg gttttgcact tctgttcatg gcagccggcc tgatcaccgc
     2761 actgtttgta aagggatatc atattccgga gctcactctt tcaaatttca ggaattttca
     2821 tgtaaacccg gataaattcc cggttttccc tatgatgttc atctctattg cctgcggtgc
     2881 catttcggga tttcattcca cccagtcgcc tctaatggcc aggtgcatta caaatgaaat
     2941 gcttggccgg agggtttttt acggggcaat ggtatctgaa ggtattattg cactgatatg
     3001 ggctgcaata ggaatgagtt tttacggggg agtgggggct ctgaatgaaa tgatgaccgc
     3061 acagaaagga aacgccgcct atctggtgaa tgaaatatcc aattccctgt tagggagatt
     3121 cggaggatta cttgcaattc tcggagtggt tgcagccccg atcacttcag gcgataccgc
     3181 attccgcagc gcccggctca ttgtggcgga tttcctgaaa atcaggcagg gtcctctttt
     3241 aaaccgcctg tatatcagcg tgccgctttt ccttgtgggg tttcttatta ccagaatgga
     3301 cttcgcggtc atatggcgct atttcgcctg gtcaaaccag accctggcaa tggttgtgct
     3361 gtggacaata acagtatatc tccttcagac gggcaggctc tatatggtaa ctctcattcc
     3421 tgcatttttc atgacggcgg tttgtgtaag ctatatcctt tttgcacccg aaggtttttc
     3481 ccttcccgga gccatatccc atacggcggg aattattgcc gctgtcatag ttctgacggt
     3541 tttcctcgtt tataaacaga aaatcgaaaa aactgctccg ggtttcggat aagcagaagc
     3601 ggataaaaag cggaggaaga aaaacagaat attgtttata tttataagca ctttataaaa
     3661 aaaggcatgt gccgggaaaa gcatatgtta aaaagcatct gctacaaaag acatacaata
     3721 aataagcatc tatggaaact gataattcac gacgcggatt tttacggaaa gctgcattcg
     3781 gaagccttgc ggcaataagc atccctgaaa tagtttccgc ggctctgccg caggaaagaa
     3841 acgggataaa acttctcaaa ggacagacca tcctttttca gggtgattcc attaccgatg
     3901 ccggcaggag caggaaggaa acgggcttca ataatgccgg agccctggga tccggatatc
     3961 caatgctggc cgctgcccgg cttcttctca gccatgccgg cctcgacctt aagatttaca
     4021 acaggggaat atcggggaat aaggtatttc agcttgctga aaggtgggat gaggattgta
     4081 ttgacataaa acctgacgtg gtcagcatat taataggggt aaatgacatc tggcataagc
     4141 tcggcggaaa ttacaacggg actgtggaaa tataccggag ggactatatt gccctgattg
     4201 agagaacgaa aaaagccctg cctgctgtta agcttattat cggtgagccc ttcggggtgc
     4261 gcggggtgaa agctgtcgac gacagctggt atccggagtt ttatgaatac cagaaagcgg
     4321 caaaagagat agcctcacag tttggagcac agtttattcc ctatcaggct gtttatgatg
     4381 aggctcagct cagggcaccg gctgcctact ggaccggcga cggggttcat gcctcacttg
     4441 caggggcgtc cctgatggca gaagcatggc tccgtatggt ggaataggga cattaagccg
     4501 ggtatcaggg gtatgactcc ggattgcagg atagtggtat aggactgagt gtcaggagta
     4561 tgactgcata ttatgggaaa tggcaaggaa tacctgaacg ggattgttgc agaagacatt
     4621 ttcaaggaac tgaaaaaaat aaaaacctga atcatgatta agacagaaac agctgtaaaa
     4681 aatgcatcgg gaaatttccc gggaatactt ttgattttat ttttcatatt tcttcccgga
     4741 atcatttttg gtcaggaaac tgtttacgga cttttatatt ccgacaataa acctgtctct
     4801 gtcactttca gggacggagt aataactgaa gtccggaaaa tcaaaaagct tccttccgga
     4861 agtgaaggac tgataatagg tcccggtctg atagacaatc agataaacgg atttgccggt
     4921 gtctctttca cttttggcgg aggagaactt acccgggatg gtgtaataaa agcgactgaa
     4981 gctatctgga agacaggtgt taccacatat ctgcccacac ttaccaccaa cagtcatgat
     5041 gtccttatga agaatttcgc cgttctcggc agtattgccg gggatgcggc cctgctggga
     5101 tcaataccgg gttttcatct tgaaggaccg tatatatcac cggtggacgg ctacaggggg
     5161 gcccatccac ttgatcatgt cagaaagcct gactggcagg agtttcttca atacagaaaa
     5221 gcagcaggga acaaaattat tcatgtcacc attgcaccgg aagttgaagg cgccatggat
     5281 tttatcgaca aatgcagggc agagggcata gtggttgcac ttggccacca taacggatct
     5341 gcaaaagaaa ttaccgaggc cattgaccgg ggagccatta tagcaaccca tctggggaac
     5401 ggctgtgcaa acatgatcaa caggcatctg aatcccctgt ggccccagct ttctgacgac
     5461 cggctgatga tcagtataat aggagacgga tttcatctca atccggaaga aataagggtt
     5521 ttttacaagg tgaagggacc ggataaaaca ataatcactt cggatgtcac cagctatgct
     5581 tcacttgaac cgggaagata tctgacagat gaaggagagg cgattgaact gacaccggaa
     5641 ggcatgctga agtatccggc acaggatgtg ctgtacggct cggcttcacc tgtgaaaaaa
     5701 ggacttgtaa acgtaatgaa atacacggga tgttccctcg aagaggcatt ccggatggca
     5761 gctttaaacc ctgcaaagtt atacggactg aacgaccggg gtgagatagc caggggcaag
     5821 agggcagata tcattttatt ccgtatcgaa aatgatgaaa tgattattaa aaagacctgg
     5881 gtaaagggaa aactggttta tcaggaataa taatattaac tcaggaacaa taatacaact
     5941 tcaggaatta tacggcatcg caggtgacaa tacacatcgc aaggccggga ctgtaatatc
     6001 atccgggcta atgttacaga atcagaaaac aggataagct gataaattac tgtacggatt
     6061 tctttttttt cagtatttct ttttctattt tacagcctta actaaaaacc gccgtgccta
     6121 tgagaaatga aatgcctgtc atttgttcat ccgtttgttt attcatttat ctgatcattc
     6181 attctctctg ttcattcaga gagaaagagt accggaatat tcacttttaa caaccttaat
     6241 ttcacttaac ttaattttat gaaaaattct tatgtctgta agacacattc actgtgtctc
     6301 aaagaattca tgaagaagct caggattatg gcactgttgt tccttttcct ggcttcctgg
     6361 ggtattgcct cagccggcag tgctgtgccg ggaaatggtg acatcaatgc agcagatatg
     6421 cagcagatca ctgttaccgg gacagtcacc gatgcttcca ccagggaacc catgccggga
     6481 gtaaacgtac ttgtaagcgg aaccactctg ggtgcactga ctgatctcag cggcagatac
     6541 agtctgaatg tacccggcag ggatgcagtt ctggtttttt cctttatagg attcatcacg
     6601 caggaacagg ctgttgcggg aaggacagtg atcaatgtag cacttgaacc cgccacggaa
     6661 gctcttgatg aagtaattgt gatcggatac ggaacccaga agaaaagcga tctgacgggt
     6721 tcggtatcca gggttacttt tgatgacaag gcaacgctgg cgaacctgaa cctgtcacag
     6781 gctatgtccg gttccaccgc aggtgtcaac atccagcagg gttccggact ggcaggagcc
     6841 gaaccaagcc ttgacatcag gggacagacc tctttgtcag caagcgacag gccgcttatt
     6901 gttctggacg gtatcatttt caacggttca atctcggata tcaatgttaa cgacgttgag
     6961 gcagtggata tcctgaaaga cgccagtgcc gcggctgttt acggatcgag gtcggccaac
     7021 ggtgtgatcc tgatcactac caaaagagga aaatcggaga aacccctcat tggcttcaac
     7081 atgtattacg gctaccagaa tatgtcaaat aatcccatga gggtgatgaa tgccgaggag
     7141 tatgccatcc ggctggttga ctactattac cagcaggatc tttatacctg gtacaaaacc
     7201 aagccgacga gctcagaggg caagcctgta aggcccgata tcaccaaccg cgaactggtg
     7261 gccgccaagc tgagaaccgc ggaggaaagg gacaactata ttgcaggcaa tgaaattgac
     7321 tgggtgaagg aggttatgcg tacggcaccc attcagaatt ataatctcag cctctcggga
     7381 aagagcgata aatccaatta cttcctgtct gcctcctatt ccgacgagaa agggattctg
     7441 agaaatgacc agttcaacag aatcaccctg cacagcaatg ttgaaagcaa aatcaccgac
     7501 tggcttacag tagggctgat ttcatcatat tcctaccgcg attattccgg tatcaatgcc
     7561 agcctgggcg atgccagaag ctgcagccct ttggcaaaca acaagatagg ccgtccggat
     7621 tatgacatgt tcctcaccgg ggaggcatac atgacctatc ctcttgttta tgaatacata
     7681 aagaacaagg atgtcaggaa caacctgttt a
//
LOCUS       DAOSPX010000143         7705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8925_length_7705_cov_3.365229, whole genome shotgun sequence.
ACCESSION   DAOSPX010000143 DAOSPX010000000
VERSION     DAOSPX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8925_length_7705_cov_3.365229"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..2432)
                     /locus_tag="PLZ75_08110"
     CDS             complement(66..2432)
                     /locus_tag="PLZ75_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOS72394.1"
                     /translation="MGNFIIKYYRQIIIICILLGLGFGSLVPFSKTDPEIRNYVPSTM
                     ESRILTDKIETEFGIQDIVMVIFRDSTVLTSDNLNRIKQIDRDISRLSGVTSRISPFT
                     VRTITSTEGMMTADPLIARVPSDPEGLAALKDEILKNRFARDIVISSDMTTASITATI
                     NQAEPETATLHKIDSIIAVHAGGSEILTGGLPYIRQHIMKDVNRDALILVPIALLLML
                     IVLKLNLNDWRSVLMPFSVVLFSTAICTGMIPLLDWRLSIISLLVPVILIAVANNYGI
                     YLVTRFQEITQRKDGTSPGNMLKELTGSLNMPILFSGLTTIAGILGLLAHSIIPARQV
                     GILAASGVTVALIMSLLYIPAMIYAQKGRIVAKKQPAGQHFIFKTWLDRLSSVIVKHP
                     GKIITVFATVTLIFALGFTFLKIETNQENYFPKKSPVRMASDLINSKFGGSQTVSVMI
                     EGDIKDPEIMNGIDRLTQRLEKTDGVGNVFSISQAVREMSKAIYTEGESMYDRIPETR
                     DGVAQMFELYNMSGDPDDFSQMMNLENTRAHVLVRLSNPDNRTINEVKAAISSDIKDI
                     PAKITVGGYAIIMTDFASSIIKGQVFSLIFAMLTVFILLALIFRSAAGGMIGTIPLAV
                     SIVILFGFMGFTGIAIDAATALLSSIMIGVGVDFTIQYIWCFNSQIVKGLSYEESIRS
                     SMRIIGRSIVINALTVMSGFSVLMFSGFASIRFFGYLVLVSISSCLLGAIILVPAIIL
                     KFRPGFVKKDMNKPKIIKHENENIASGIPGVAAFAAVSPAAGRGSDNE"
     gene            complement(2437..3684)
                     /locus_tag="PLZ75_08115"
     CDS             complement(2437..3684)
                     /locus_tag="PLZ75_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72395.1"
                     /translation="MDKNDDKPYVSSAFSDFGLKAEIKDNIRFKAFADIRFRYGTEFL
                     EPVKNINLREAYVKVNGKFWDITAGQTIVKWGRTDFSNPTSKLNPQNYISRSPDPEDM
                     DMGNLLAELKIYPAPFFSLQAVAVPYYRPSTLLIDPLPLPGWIKINQLPALLTSRDMF
                     SYGLKAEFRLSGTDMSLSWFNGYDPMPGAALTAFSLDMSGSFPSPSAEISMTPYKIQN
                     FGIDFENTIGSFGVRGEGSWSVPYKSHKTAEYVPLEEVKYVAGIDWMPGDWRLTAEYS
                     GKTILGFEAPAVEPLIGAELDMAKLSALLADPGFVLDDYVRQQVSSFNRLYNYQMEKT
                     YHSAGLRIERELFYGKVVPSVFALYNFTSRDLLLMPEIRYKPADGLTISAGGDIYSGK
                     KGSVHDLVNDFMNCIRIAVRVDF"
     gene            complement(4010..>4444)
                     /locus_tag="PLZ75_08120"
     CDS             complement(4010..>4444)
                     /locus_tag="PLZ75_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOS72396.1"
                     /translation="IFDPEIETNGDEKKETEKYIKSGLKDEQAQEYLEKLISVMETDK
                     PFLNRDLSIQDLALITDIPRHHITQVLNEVHGKNFFTFINEYRVEEVINRFRDPANNN
                     FTILAIAFDAGFNSKATFNSIFKLQTGMTPSEYREKLTGIRG"
     assembly_gap    4445..4454
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4455..5125)
                     /locus_tag="PLZ75_08125"
     CDS             complement(<4455..5125)
                     /locus_tag="PLZ75_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72397.1"
                     /translation="MVNIDPILYIGISQSFFAGLLISTKKPFTTANRILAAWVFLICI
                     ELIFALINSTVIEMYSFPFVSFTYGPLLFLYIYFMVNPGKKFNWLSLLHFIPFITFFI
                     VSVIFRSKPLLRDLRSFFVPDRFITLRIVYSTAMFTSLTVYSTLAFIMIRKHQKNLKN
                     LVSYTSNVMTLNWLKVISISLYVSFLILFILGGLNMIGDIIPFDPYFVVFGFISVFSF
                     VYSFYG"
     gene            5288..6163
                     /gene="sucD"
                     /locus_tag="PLZ75_08130"
     CDS             5288..6163
                     /gene="sucD"
                     /locus_tag="PLZ75_08130"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOS72398.1"
                     /translation="MSVLLNKDSRIIIQGFTGREGTFHSALMIEYGSKVIGGTTPGKG
                     GQTHLGLPVFNSVQESQDHAPADVSVIFVPPPFAADAIMEAASAGIGLIVAITEGIPV
                     SDMIKVMEYIRYRDCRLIGPNCPGIITPGESKAGIMPGFIHRKGSIGVVSRSGTLTYE
                     AVDQITKLGMGQSTCIGIGGDPIIGTSVLDAVKLLMADDETEAILIIGEIGGNMETEA
                     AEWIKANGTKPVIGFIAGRTAPRGRRMGHAGAIIGGKADTAEAKMTVMRDCGIFVVES
                     PAEIGASVAKTLRPA"
     gene            6264..7010
                     /gene="fabG"
                     /locus_tag="PLZ75_08135"
     CDS             6264..7010
                     /gene="fabG"
                     /locus_tag="PLZ75_08135"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOS72399.1"
                     /translation="MGLLAGKTALITGASRGIGRAIALLFAAEGADIAVTNIADDEEF
                     RETVSEIEKLGVKAKGYVADAASFADSQKTADEIIKDFGRIDILVNNAGITRDTLLLR
                     MTEEQWDSVIAVNLKSVFNMTKACLMPMVKQTGGSIINMSSVVGVSGNAGQSNYSASK
                     AGILGFTKSVAKEVGSRNIRCNAIAPGFILTEMTEKLPDDVKKEWTARIPLKRGGTPE
                     DVANTALFLASDLSSYISGQIIHVCGGMLT"
     gene            7144..>7705
                     /locus_tag="PLZ75_08140"
     CDS             7144..>7705
                     /locus_tag="PLZ75_08140"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HOS72400.1"
                     /translation="MCNYVLLLFILIPVAGYLLQRYLEGLNIRMWSDKLPEKLIGICD
                     EKEYHRTQHYYRENLRLSVWSSTLNLTVIIIMLATGGFALVDNIARSLSGNNVVIALI
                     FMGIIGIASELINLPFAWYDTFVIEKKFGFNTMTKRTFITDHLKAWFISLLIGIPVLA
                     IVTWLYYRMDKGFWIYAWLLVTAFTVF"
BASE COUNT         2087 a         1680 c         1804 g         2124 t
ORIGIN      
        1 ttttctcggt tatcgacaga ttaatggtcg cctgcattga agaaagaagg ctcaggtcac
       61 gggttttact cattatctga cccgcgtcca gctgctgggc tgacagctgc aaaagcggcc
      121 acaccaggaa taccagaagc gatattttcg ttttcatgtt ttattatttt tggtttattc
      181 atatcttttt tcacaaaccc gggcctgaat ttcagtataa tggccggaac cagaattatt
      241 gcgcccagaa ggcacgagct gatggatacc aggacaaggt atccgaagaa gcgtattgag
      301 gcaaatcctg aaaacatcag cacggaaaaa ccggacatca cggtcagggc gttgatcact
      361 atgctacggc cgataatcct cattgaggac ctgatggatt cctcgtacga caggcctttt
      421 acaatctgac tgttgaaaca ccatatatac tgaatggtaa aatccactcc cacacctatc
      481 attatcgatg acaggagggc ggttgccgca tcaatggcaa ttcctgtaaa tcccatgaat
      541 ccgaacaata tgacaattga tacggccagt ggtattgttc ctatcattcc tcctgccgct
      601 gaacggaata tcagtgcaag caggatgaaa accgtaagca ttgcaaaaat cagggaaaat
      661 acctggccct ttattattga tgaagcaaaa tcagtcatta ttatggcata tcccccgacc
      721 gtgatttttg caggtatgtc ttttatatca gatgatatag ccgccttgac ttcattgatg
      781 gtcctgttgt caggatttga cagtcttacc aggacatgtg cccgggtatt ctcaaggttc
      841 atcatctgac tgaagtcatc cggatcaccg ctcatattgt ataattcaaa catctgtgcc
      901 actccgtcgc gtgtttccgg aatcctgtca tacatgcttt caccttcggt atagatagcc
      961 ttgctcatct cccttacggc ctgtgaaatg gaaaaaacat ttccgacccc gtccgttttt
     1021 tcgagtcttt gtgtaagcct gtcaatacca ttcattattt ccggatcctt tatatctcct
     1081 tcaatcatca ccgagacggt ctgggacccg ccaaactttg aatttatgag atcggaagcc
     1141 attcgtacag gactcttttt cgggaaataa ttttcctggt tggtttctat tttcaggaaa
     1201 gtgaagccca gtgcaaatat cagggtgaca gttgcaaaga ctgttattat ctttccggga
     1261 tgtttcacta ttaccgacga cagcctgtca agccatgtct taaagatgaa atgctgtcct
     1321 gcaggctgtt ttttcgccac tatgcgtcct ttctgtgcat aaatcatcgc agggatatac
     1381 agcaggctca ttatcaaggc tactgtcacc ccgcttgctg ccagtatccc gacctggcgg
     1441 gcgggaatta tggaatgggc aagtaatccg agaatgccgg caattgttgt cagaccgctg
     1501 aaaaggattg gcatgttaag tgagccggtg agctccttca gcatgttccc aggagaagtt
     1561 ccgtcctttc tttgtgtgat ttcctgaaat ctcgtcacga gatatatccc gtaattattt
     1621 gccacggcga tgagtatcac gggaaccagc agactgatta ttgacagccg ccagtccaga
     1681 agcggaatca ttcccgtaca tattgccgtt gagaacagaa ccacgctgaa cggcatcaga
     1741 accgatctcc agtcgttaag gttcagtttc agcacaatca gcatcaggag aagggctatc
     1801 ggcacgagta tcagtgcatc cctgttcaca tccttcataa tatgctgacg gatatatggc
     1861 agtccgcctg tgaggatttc agaaccgcct gcatgcacgg caatgattga atcaattttg
     1921 tgcagggttg cagtttccgg ttcagcctgg tttattgtgg cagtaataga ggctgttgtc
     1981 atatccgatg aaatcacgat atcccttgca aaccggtttt tgaggatttc atctttcaga
     2041 gcggccaatc cttccggatc agaaggtacc cgtgctataa gcggatcggc agtcatcatc
     2101 ccttcagtac tcgtaatggt cctgaccgtg aagggggata ttctgcttgt cacaccgctc
     2161 agtctggaaa tatccctgtc tatctgtttt atcctgttca gattatcgga agtaagaacg
     2221 gtactgtccc tgaagatcac cattactata tcctgtatcc cgaattcagt ttcaatcttg
     2281 tcggtcagta ttcttgattc cattgttgac ggaacataat ttcttatttc gggatcggtt
     2341 ttcgaaaacg ggacgaggga tccgaagccg agtccgagaa ggatacatat aataattatc
     2401 tgcctgtaat atttaatgat gaaatttccc atagtattaa aaatcaactc ttactgctat
     2461 tcttatacaa ttcataaaat catttacaag gtcatggaca gagccttttt tacctgagta
     2521 aatatctccg cctgccgaga ttgtcagtcc gtcggcgggt ttatacctga tctccggcat
     2581 aagaagaagg tcgcgcgagg taaagttgta aagcgcgaaa accgaaggaa ccaccttgcc
     2641 gtaaaagagt tccctttcta ttcttaaacc ggccgagtga taggtttttt ccatctggta
     2701 gttgtagagc ctgttgaagg agcttacctg ctgcctgaca taatcgtcga ggacaaagcc
     2761 aggatctgcc agcagtgctg acagttttgc catatcaagt tccgcaccta tgaggggttc
     2821 cacagccggt gcctcaaaac ccaatatggt ctttcccgag tattctgccg ttaagcgcca
     2881 gtctcccggc atccagtcga tacctgcaac atattttact tcctcaaggg gtacatattc
     2941 tgctgtttta tgcgatttgt aaggaacact ccatgaacct tcgcccctga cgccaaagct
     3001 tcctattgtg ttttcaaagt cgataccgaa gttttgtatt ttgtagggag tcattgagat
     3061 ctccgcggaa ggagagggga aggatcctga catgtcgaga ctgaatgccg tgagggctgc
     3121 gcccggcatg ggatcgtagc cgttgaacca cgacaggctc atgtcggttc ctgaaagccg
     3181 gaattccgct ttcagtccgt agctgaacat atcccttgat gtaagcagcg ccggcagctg
     3241 atttattttt atccagcccg gcaggggcag gggatcgata agcagggtgg aaggcctgta
     3301 atagggcact gcaactgcct gaaggctgaa gaatggtgca ggatatattt tcagttctgc
     3361 aagcagatta cccatatcca tgtcttccgg atcgggggaa cgtgatatgt aattctgcgg
     3421 attgagcttt gatgtgggat tggaaaaatc agtccgcccc catttgacaa tggtttgtcc
     3481 ggcagtgata tcccagaatt tgccgttcac ctttacatat gcctccctca ggtttatatt
     3541 tttaaccggt tcaaggaatt cggttccgta tctgaatctg atatcagcaa atgccttgaa
     3601 acggatgttg tcctttattt ccgccttcag cccgaaatcc gagaaagcac ttgagacgta
     3661 aggtttatca tcattcttat ccatggttcc gtacaggccg cctcttacaa acccgtaaaa
     3721 aacggacggt ttctcaggat tctccgtcaa aagggataaa ccgtcatcct gggcagacag
     3781 gaataaagga aaaaatagaa atagagtgag aaatgaaatg caaattctgt tcatctgtgt
     3841 tttttataca aatgaacagt atttaacaag gtccgtcgtc tgaaccaatt gtcccgtacc
     3901 tgtaaaatcc gtattacagg tacgtgccgt ccgggacaga ccccgttttt acaatccggt
     3961 tccggaattt gtgatttatg ttttactcct tactggcatt cccgcctgtt taccctcgta
     4021 ttccggtcag tttttccctg tattcactcg gagtcatccc ggtctgcagc ttaaaaatcg
     4081 aattgaacgt tgctttggaa ttgaaacccg catcaaaggc aatggcaaga attgtaaaat
     4141 tattgtttgc aggatccctg aacctgttta tcacttcctc caccctgtac tcatttatga
     4201 aagtaaagaa gtttttgcca tgtacctcgt tgaggacctg tgtgatgtgg tgacggggta
     4261 tgtctgtaat gagtgccaga tcctgtatgc tgagatccct gttgagaaag ggcttgtccg
     4321 tttccataac agaaatgagc ttttcaagat attcctgagc ctgttcatcc ttcaggccgc
     4381 tttttatata tttttccgtt tctttctttt catcgccgtt ggtttcaatt tccggatcga
     4441 atatnnnnnn nnnnccgtaa aaactgtata cgaatgaaaa gacagaaata aatccaaaaa
     4501 caacaaagta cggatcaaag ggtatgatgt cgccaatcat gtttagtccg ccgagaatga
     4561 acaggatcag aaaagacacg tagaggctta ttgatatcac tttcagccag ttcagtgtca
     4621 ttacatttga tgtgtatgat accaggttct tcaggttttt ctggtgtttt ctgatcatta
     4681 tgaatgccag agtactatac acggtaagtg aggtgaacat ggcagtgctg tagactatcc
     4741 tgagggtgat gaatctgtcg ggaacaaaga aacttctcag atcacgcaga aggggctttg
     4801 atctgaaaat aaccgaaacg ataaaaaatg ttatgaatgg tatgaaatga agaagggaaa
     4861 gccagttgaa tttctttccc gggtttacca tgaaatagat atagagaaat aacaggggtc
     4921 cgtaggtaaa ggaaacaaac ggaaaggaat acatttctat caccgttgaa ttaatcaggg
     4981 caaaaatcag ttcgatgcag ataaggaata cccatgccgc aagtatcctg ttggcagttg
     5041 taaatggctt ttttgttgaa atcagtaatc ctgcaaaaaa agactgtgag atgcctatgt
     5101 atagtatggg atcaatattc accatcctgt tgtacgaaat ttatttcaaa gatactaata
     5161 agggctaaaa ttaataagta tttttgagag gtatccggga ttgccgggaa gtgctgacct
     5221 gcgtccatac tgtaactatc gcggtcatcg ccggatgtga cgaaatattt aaatctgatt
     5281 tcatgttatg agtgttcttc ttaataaaga ttccagaatt attatacagg gttttaccgg
     5341 aagggaggga acgtttcatt ctgccctcat gattgaatac ggttcgaaag tgattggcgg
     5401 cacaacaccg ggcaagggag gacagactca cctgggcctg cctgttttta acagtgtaca
     5461 ggaatcacag gatcatgcac cggccgacgt gtcggttata tttgttccgc cgccctttgc
     5521 tgcggatgcc attatggaag cagcctcggc agggataggg ctgattgttg caataacgga
     5581 aggcattcct gtttccgaca tgattaaggt tatggaatat ataaggtacc gcgactgccg
     5641 gctcatcgga cccaattgcc cggggataat cacaccggga gaatcaaagg ccggcataat
     5701 gccaggattc attcacagga agggcagcat aggcgttgtt tcccgttccg ggacactgac
     5761 ctatgaagcg gttgatcaga tcacgaagct gggaatgggg cagtcaacat gtatcgggat
     5821 aggaggagat cccatcatcg gaacatccgt tctcgatgct gttaagctgc ttatggcgga
     5881 tgacgaaaca gaagccattc ttattatcgg ggagataggc ggaaatatgg aaaccgaggc
     5941 agctgaatgg ataaaggcaa acggcaccaa gccggttatt ggctttattg ccggccgcac
     6001 cgctccccgg gggagaagga tgggccatgc cggagcaatt atcggaggaa aggccgatac
     6061 agccgaagcc aagatgacgg tgatgaggga ctgcggcatc tttgtggttg aatcacctgc
     6121 tgaaataggg gcttccgtgg caaagaccct gcggccggcc tgaagctgta ctgcgaagcg
     6181 gacccggatt gtcaggcttt caggaatccg gataacatta aagactttat tttatctttg
     6241 cggctgaatt aaaccagaac gatatgggat tattagcagg aaaaacagct ttgataaccg
     6301 gagcctcccg gggtatcggc agggccatag ctcttctttt cgcagccgaa ggggcagata
     6361 ttgccgtaac caacattgca gatgatgaag aattcaggga aaccgtcagc gagatagaaa
     6421 aactcggtgt aaaggcaaag ggatatgttg cagatgcagc cagttttgct gattcgcaga
     6481 aaactgccga tgaaattata aaggatttcg ggcggataga tattcttgtc aacaatgccg
     6541 gaattaccag ggataccctt ctgctcagaa tgactgaaga gcagtgggat tctgtaattg
     6601 cagtaaacct gaaatcggta ttcaacatga caaaagcctg tctgatgcct atggtgaagc
     6661 agacgggcgg ttcaatcatc aacatgagtt ccgttgtggg tgtttcggga aatgccggcc
     6721 agagcaacta ttcagcgtcc aaggcgggca ttctcggttt cacaaaaagt gttgcaaaag
     6781 aggtgggttc gagaaatata agatgcaatg caatagcacc cggattcatt ctcaccgaaa
     6841 tgacagaaaa attacccgac gatgttaaaa aagaatggac agccaggata cctttaaaac
     6901 gaggcggaac ccctgaggat gtggcaaata cggctctttt tctggcttcc gatttatctt
     6961 cttacataag tggacaaata attcatgtgt gtgggggtat gttaacttaa atttttttat
     7021 tatatttaca acatcgcagg gtgtctatcc ttttagcacg aggaaatgca gcgagacata
     7081 tttattccac acttatttta aacaggcgcc ctgcttcttt tatatgtaac agtctgatcc
     7141 tgaatgtgta attacgtcct cctcctgttc attctgatcc ctgttgccgg atatctgctc
     7201 caaagatacc tcgaaggtct caatatcaga atgtggtcag acaagcttcc tgaaaagctt
     7261 atagggatat gtgatgaaaa agaatatcac agaacgcaac attattacag ggaaaatctc
     7321 aggctttcgg tctggtcatc cacactgaac cttacagtaa tcataattat gctggccacc
     7381 ggcggttttg ccctggtcga caacattgca agatcgttga gcggaaacaa tgtcgttata
     7441 gcgctgattt tcatgggaat cattgggatt gcatcggagc tgataaacct gccattcgca
     7501 tggtacgaca cttttgtgat tgaaaagaaa ttcggcttca atacaatgac gaaacgcact
     7561 tttataaccg accacttaaa agcatggttc atatcccttc tgatcggcat ccctgtgctg
     7621 gcaatagtca catggttata ttacaggatg gacaagggat tctggatcta tgcctggctt
     7681 ctggtaacag ccttcacagt gttta
//
LOCUS       DAOSPX010000144         7696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8940_length_7696_cov_4.251145, whole genome shotgun sequence.
ACCESSION   DAOSPX010000144 DAOSPX010000000
VERSION     DAOSPX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8940_length_7696_cov_4.251145"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLZ75_08145"
     CDS             complement(<1..260)
                     /locus_tag="PLZ75_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOS72401.1"
                     /translation="MPKIHNIIPAEVRKPQFIEFGKIPVNQYSRTIEEEKQNYSSADF
                     VRIYRDMVIIREFETMLNLIKTTNEYRGISYNHPGPAHLSIG"
     gene            complement(288..1571)
                     /locus_tag="PLZ75_08150"
     CDS             complement(288..1571)
                     /locus_tag="PLZ75_08150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOS72402.1"
                     /translation="MATPVTMPKLGQSVETCIITRWFKSTGDKVSTGDILFSYETDKA
                     AFDMESPADGILLDIFFNEGDEVPVLANVAVIGEKGEETGSFRPGSSRPEDSPRTGTQ
                     TRTGIQADADIQPGAGIQANTGIQAGTVSQTAADTRLRISPRARRLALEKGLDYSRLR
                     GSGPNSRIMVSDIEKLLLTGTGDIIPDHTEDKEYTDEAVSNVRKLIAKAMYNSLQNSA
                     QLTHHMSADARKLLAERKRNKEAIASGSTTVELTLNDMVCWCVVQALKKFPEMNSHFL
                     GETIRKFKRIHLGMAVDTKRGLMVPSVRNAESLKLTDLAQKLKSVAEACRKGNINPEL
                     ILSTSASFTVSNLGSYGVEMFTPVLNLPQVAILGVCSIINRPADLGNNIFGFIPYIGL
                     SLTYDHRAVDGGPATLFLKEIRKQIENFNPGQEIY"
     gene            1809..2525
                     /locus_tag="PLZ75_08155"
     CDS             1809..2525
                     /locus_tag="PLZ75_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOS72403.1"
                     /translation="MELSEKIPQYKRLYEILRRHIIEGVYNEGDLLPSENELCRLHGV
                     TRPTVRQALSNLANDGYISRHQGKGSIVHLLPREIGILSVAGTTSAVGDRNLKTKIIV
                     KPVIVPWDDDFMFPLSDLEKESGCVNMERLRLLDDSPIFYDISYIANINLPRITSRKF
                     ENRSLFRILREHYRIEIKGGEQRIKAIPASVKTGRYLQLKAGEPVLHLERKMETSSPG
                     LFLYSSIFCNTKKYSIFGRF"
     gene            2567..3892
                     /locus_tag="PLZ75_08160"
     CDS             2567..3892
                     /locus_tag="PLZ75_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72404.1"
                     /translation="MENQKTKWSRRRFINTALAGVAGAAVIPHLSGCRGPAAASDEIR
                     LGFIGLGRQAMFLLNGFIQIPGVKVLAGCDVYGIKRKRFENRVSEYYTNAGVKFRIDS
                     YERYQDLLGRKDIDAVIIAVPDHSHAMIAIAACIAGKDVYLEKPMTFTIREGQELRRI
                     VRENNRILGVGSQQRSSEEFKHAVKMVRDGAIGSISRVYAYVGAPPVPYDLPEETLPA
                     DLNWDLWLGPLPASIHYNSQLNPPISLDPPKDEEIWGAWRWYKEMGGGFTTDWGAHMF
                     DIAQWGLGMDRSGPIEISPIGDGTEFISWKYANGVIMTSEPFDEKLTKGVKFIGDKGW
                     IEVARGYYNASDPELNPPAEATEVEGPYETRIPHQVDFIDAVKKRVDPVVPVEIGHSS
                     CTVCTLGNIACDLKRTIKWNPDTETFVDDSDGAATKLLHYEYRNPWKLL"
     gene            complement(4001..4198)
                     /locus_tag="PLZ75_08165"
     CDS             complement(4001..4198)
                     /locus_tag="PLZ75_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOS72405.1"
                     /translation="MKGTVKWYDRVKGYGFIQTEDGRDIFVHRSGLEASHTNLEAGQA
                     VEFEIEQKEKGPVAVRVRKAE"
     gene            complement(4221..4535)
                     /locus_tag="PLZ75_08170"
     CDS             complement(4221..4535)
                     /locus_tag="PLZ75_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOS72406.1"
                     /translation="MNIYVGSLNFKMTEADLREKFEEYGEVASAKIIFDKYSGKSKGF
                     GFVEMPNEAEAKKAIEELNGAEIHGRKIIVNESIERPERRSNFRGGDNSRRGGYSRRE
                     NH"
     gene            4847..5236
                     /locus_tag="PLZ75_08175"
     CDS             4847..5236
                     /locus_tag="PLZ75_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HOS72407.1"
                     /translation="MGKGDRKSKRGKIFLGTYGARRRRKKIKNTDSVQLQSVREKDTR
                     GRRTVRGKKTESEAVKTVEETAGLKDREVSEEKATADLTAGKEEKSSGEATVPEGKTL
                     EEKPEKETKKAAAEKKASKSEESGKAS"
     gene            5361..6275
                     /locus_tag="PLZ75_08180"
     CDS             5361..6275
                     /locus_tag="PLZ75_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOS72408.1"
                     /translation="MGNKDNTIFLAIIACLLWSTVYAGIKIGLQYDSALHFAGLRFII
                     SGLMIFPFTVKPGLFIKTLRENRKIVIWVTFLQTLVNYILFYIGLDLVPGALGAVIVG
                     SQPLVTAVVAAVMSKEEKLTRRKIVTVFSGIAGVVFISAGRQAFHLGSAVEMLGVVMI
                     LGANIATAVSNVMVSFKSRGLNPLILSSTSLFSGGIIIFLLSIPLEGLPDYSNMPLQY
                     WLILIWLGFVSAFAFSLWYKLLQRPHVKVSEINMWKFIIPVAGAILSWVLVPGEVPEF
                     LTVAGMVIITLSLLLFYKNENGYLIRLK"
     gene            6272..>7696
                     /locus_tag="PLZ75_08185"
     CDS             6272..>7696
                     /locus_tag="PLZ75_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS72409.1"
                     /translation="MKKRLKAILVYSLFWIAFFIAARLLFIITKFREAVEFSPAQLFS
                     TFRHGMKLDLSATAYILVIPVLLMIPGIFLEGRWFRRFMRYYTIIITGISSLIVVSDT
                     LLYKYWGFRMDHTPLLYLRTPGAAMASVSTFQIIAVAAGIILLALLFIFLYYRFVERY
                     FEISGKIRFRFPALLFFVICWGSLLIPIRGGVGIAPINAGTVYFSKDMFLNHSAINVV
                     WNVGNSLANNKPVSNPYQFYDTSVARQLTASLTADSGSPMILVNDSRPNILIFVLESF
                     GSAMIGPLGGDPLTTPNFNRYVKDGILFTRFFASGNRTDKAVPAILCGYPAQPATSIM
                     KEPKKTQSLPGLVKMMNSKGYHTSFWYGGEINFANFNSFVINAGFMSVITRDNFSPGF
                     YNSKWGVHDHVLLEALQDSMKNIREPFLKVVLTLSSHEPFDVPMTPVFRGNDDLTRFR
                     NSIYYTDRSLGQFLDQAKTTEWWKN"
BASE COUNT         2028 a         1666 c         1783 g         2219 t
ORIGIN      
        1 tgccctatgg acaaatgtgc gggacccgga tgattatagc ttatgcccct gtattcgttc
       61 gtggttttga taagattgag catggtttca aattccctga ttatgaccat gtctctgtaa
      121 atcctcacaa agtccgcaga actgtagttt tgtttctcct cctctatagt cctggagtac
      181 tggttaaccg gtatttttcc gaattcaatg aattggggct tgcggacctc agccgggatg
      241 atgttatgaa tttttggcat ttttcagagt gttagggtga attttcatta atatatttcc
      301 tgtccgggat tgaaattctc tatctgtttc ctgatttcct taaggaaaag agttgcgggt
      361 cccccgtcaa ccgccctgtg gtcataggtg agggaaagcc cgatgtaggg tatgaatccg
      421 aaaatattgt ttcccaggtc agccggtctg tttatgatgg aacaaacccc cagtatggca
      481 acctgtggaa ggttcagtac cggagtaaac atctcgacac catagcttcc aaggttggat
      541 accgtaaagg atgccgaggt actcaggata agttccgggt ttatgtttcc tttccggcat
      601 gcttccgcaa ctgatttgag cttctgtgcc agatcagtca gcttaaggct ttccgcattc
      661 cttaccgaag gaaccatcag gcctcttttt gtgtcaacag ccatacccag gtgaatccgt
      721 ttgaatttgc ggattgtttc ccccaggaaa tggctgttca tttccgggaa tttcttcagt
      781 gcctgaacaa cacaccagca taccatatca ttaagcgtca gttcaacggt tgtgctgccg
      841 gatgcgatgg cctccttgtt cctttttctt tctgcgagca gttttctggc atctgcactc
      901 atatgatggg taagttgtgc ggaattctga agcgagttat acattgcttt ggcaatcagc
      961 tttctgacat ttgaaactgc ttcatctgtg tattccttgt cttctgtatg gtccgggatg
     1021 atgtctcctg tgccggtcag aagcagtttt tcaatgtcac tcaccatgat tctgctgttc
     1081 gggcctgatc cccggagcct gctgtagtct aatccctttt ccagggcgag ccgcctggct
     1141 ctcggagata tccttaaacg ggtgtctgcg gctgtctgtg aaactgttcc cgcctgtatg
     1201 cctgtatttg cctgtatacc tgcgcctggc tgtatgtccg catctgcctg tatgcctgtc
     1261 cgggtctgtg tgccggtccg cgggctgtct tccggccggg aactgccggg tctgaaagat
     1321 cccgtttctt ctcctttttc tcctattact gcaacattgg cgagcacagg aacctcatcg
     1381 ccttcattaa agaaaatgtc gagcaggata ccgtctgcag gagattccat atcgaaggcg
     1441 gccttgtccg tttcgtagga aaacagtatg tcgccagttg agactttgtc acccgttgat
     1501 ttgaaccagc gggttatgat gcatgtttca actgactgtc cgagttttgg cattgttacg
     1561 ggtgtagcca tatttgttgc tgcttattgg ttgttgttta aagatatttc tttatgatga
     1621 ccattagttt ccgtgcattt tccggttgtt gccgggctta aatattgtca ttgaacttgt
     1681 tatgatgtgt tgagtgaaac ttaaaaaaga taatcgcatc tgattaagat tcagagcgta
     1741 caaaaataaa ttaaatagct tgtatttaca agtgaaaagc aatatattta cgggaaaatg
     1801 tacttccaat ggaattgtct gaaaaaatac cgcagtataa aaggctttac gagatactcc
     1861 gcaggcatat tattgaaggg gtttacaatg agggagatct tcttccgtcg gagaatgagc
     1921 tgtgccgcct tcatggagtg acccggccga cggtaaggca ggcgctgagc aaccttgcca
     1981 atgacgggta tatcagccgg catcagggta aggggagcat tgttcatctt ctgccccgtg
     2041 agatcggcat cttgtcggtt gcagggacga catccgcggt tggtgaccgt aatctcaaga
     2101 cgaaaattat agtaaaaccg gtgattgtgc cctgggatga tgatttcatg tttccccttt
     2161 cggatcttga aaaggaatcg ggatgtgtaa atatggaaag gctgaggctg cttgatgatt
     2221 ctcccatatt ttatgacata agctatatag ccaatatcaa cctgcccagg attacttcca
     2281 gaaagtttga gaaccgctct cttttcagga ttctgagaga gcattaccgg attgaaatca
     2341 aaggtggtga gcaaaggatc aaagccattc ccgcttcagt gaaaacaggc cggtacctgc
     2401 agctgaaagc aggagaaccg gtattgcatc ttgaaagaaa gatggaaacc agcagtcccg
     2461 gcttatttct ttattcttca atcttttgta atacgaagaa atattctata ttcggaaggt
     2521 tttagcaact gtttttttca atataaatca ttaattataa cctgttatgg aaaatcagaa
     2581 aacaaaatgg tccagacggc gctttataaa cactgcactg gcaggggttg cgggcgcggc
     2641 tgttattcct catctttcag gatgccgggg accggcagca gcttcggatg aaatccgcct
     2701 gggttttata ggactgggcc ggcaggcaat gtttctgctg aacggattca tccagatacc
     2761 cggcgtaaag gttcttgcgg gatgtgatgt gtacggaatc aaacgcaaac gcttcgagaa
     2821 cagggtttcg gaatattata cgaatgcggg tgttaaattc aggattgaca gctatgaaag
     2881 gtatcaggat ttgctcggac gcaaagatat tgatgccgtg attattgcag ttcctgatca
     2941 cagtcatgcc atgatagcca ttgctgcatg cattgcaggc aaggatgtat accttgaaaa
     3001 acccatgaca tttacaatca gggaaggtca ggaattgagg aggattgtcc gtgagaacaa
     3061 cagaatcctt ggtgtgggaa gccagcagcg ttcaagtgag gaattcaagc atgccgtcaa
     3121 aatggtcagg gatggtgcca ttggcagcat atcaagggtt tacgcctatg tcggagcacc
     3181 gccggttccc tatgatcttc cggaagaaac acttcctgca gatctcaact gggatctgtg
     3241 gcttggtccg ctcccggcct caattcacta taacagccag cttaatcctc caatatcgct
     3301 tgatcctccc aaagatgagg agatatgggg tgcatggaga tggtacaagg agatgggagg
     3361 cggctttaca actgactggg gtgcccatat gttcgatatt gcccagtggg gtctcggaat
     3421 ggacagaagc ggtccgatag aaatctcccc catcggtgac ggcactgaat tcatttcatg
     3481 gaagtatgcc aacggggtga ttatgacatc agagcccttt gatgaaaaac tgacaaaagg
     3541 cgttaagttc atcggggaca agggctggat agaagttgca agaggctatt acaacgcctc
     3601 ggatcctgaa ctgaatcctc ctgcggaagc aaccgaggtt gaaggaccct atgaaacaag
     3661 aatacctcac caggtcgatt ttatagatgc agttaagaag cgtgttgatc ccgtcgttcc
     3721 cgtggaaatc gggcacagca gctgtacggt ctgtaccctg gggaatattg catgtgacct
     3781 gaaaaggaca attaaatgga atcctgacac ggaaacattt gttgacgaca gtgatggtgc
     3841 tgccacaaaa ttgctgcact atgaatacag gaatccctgg aaactgcttt aaaagaactg
     3901 tgcaggcaaa aaattatttg acggtcattc agtcatttga aaaaaggagg ctgtaccccg
     3961 gtacagcctc ctttttcatt aatcaataat aagagcagga tcattctgct tttcttaccc
     4021 ttacagcaac aggacctttt tccttctgct caatttcaaa ttcaacagcc tggcctgcct
     4081 caagattggt gtgagaagcc tcaagtcctg aacggtgtac aaaaatgtcc ctgccgtctt
     4141 cagtttgtat gaagccgtaa cccttgactc tgtcgtacca ttttacagta cccttcataa
     4201 aatcatattt ttatttcctg tcaatgattt tctcttctgg aatatcctcc acggcggcta
     4261 ttgtccccgc ctctgaaatt acttcttctt tctggccttt caattgattc gttaacaatg
     4321 atttttcttc catgaatttc tgctccattg agttcttcaa tagccttttt ggcttcggct
     4381 tcgttcggca tttcaacgaa gccaaatccc ttgctttttc cggagtattt gtcaaaaata
     4441 atttttgcgg aggctacttc gccatactcc tcaaattttt ccctaaggtc tgcttcagtc
     4501 attttgaaat taagacttcc tacataaatg ttcataaatt ctgttattaa aataagtatt
     4561 ttgaccaggt caaaggtctg attttttttc ggaaagaaaa aatattagga tacaattagt
     4621 taaattttaa aactcttttt ttctgcccct tttaggttta aatcatccgg ccgttatttg
     4681 ttccctgtgc tgaacagatt gtcctgtaag cgccgtaagc ataaaagatt ccaaatattc
     4741 agtaacagtg acggccaaaa tagatttatc ctaaatcata aataaagaat attattaact
     4801 ttgccgcaac ctcaaaaggg gaatgaacaa atcaaatccg ataattatgg gaaaaggtga
     4861 cagaaaatca aagagaggta aaattttcct tggaacatat ggtgccagac gccggagaaa
     4921 gaaaataaaa aatactgatt cagtccagtt gcagtctgta agggagaagg atacaagggg
     4981 aagaaggact gtcaggggaa aaaagactga gagcgaagct gtaaaaacag tggaagagac
     5041 tgctggattg aaagacaggg aagtgtctga agaaaaggcc accgcggacc tgacggccgg
     5101 caaggaagaa aaatcctccg gggaggcgac agtcccggaa gggaaaaccc ttgaagaaaa
     5161 acctgaaaag gaaacaaaaa aagctgcagc agaaaagaaa gcctctaaga gtgaagaatc
     5221 cggaaaggca tcctgatctg aacaactccc gtcacaagaa gctttttccc tcaaaacccg
     5281 caatccggtc gcattcaaag tcatgttgat tttcagttat cctgcaggca gtacaacaaa
     5341 attgacaagc aaaaattttt gtggggaata aagacaatac aatatttctt gccattattg
     5401 cctgcctcct gtggtcaacg gtttatgcgg gcattaaaat cggactgcaa tatgactctg
     5461 cccttcattt cgcaggttta agattcatta tatcgggtct gatgatcttt ccctttacgg
     5521 taaaacccgg attattcatt aaaactctcc gcgaaaaccg gaaaatagtc atttgggtca
     5581 cattcctgca aaccctggta aattacatac ttttttatat cggtcttgat ctggttccgg
     5641 gtgccctcgg agcagttatt gtaggttccc agcctctggt gactgcagtt gttgcagctg
     5701 taatgagtaa ggaagaaaag cttacacgca ggaaaatagt aacagtgttt tccgggattg
     5761 caggtgttgt ttttatcagt gccggcagac aggcctttca ccttggaagt gcagttgaaa
     5821 tgctgggagt tgttatgata ctcggggcaa atattgcgac tgctgtaagc aatgttatgg
     5881 tttcatttaa aagccgcgga ttaaatccat tgatactgag ctccacgtcc cttttctcgg
     5941 gaggaatcat tatattcctt ctgtcaatac ccctggaggg acttcctgac tatagcaata
     6001 tgcctttgca atactggctc atcctgatct ggctcggatt tgtttcagcc tttgctttct
     6061 cactctggta caaattactg caaaggccgc atgtgaaagt ttcggaaatc aatatgtgga
     6121 aattcattat acctgttgcc ggcgctattc tcagctgggt acttgttccc ggtgaagtgc
     6181 cggaatttct tacggttgcc ggaatggtaa tcataacact atctttgctt ctgttctata
     6241 aaaatgaaaa tggctatctt atccggctga aatgaaaaaa agacttaagg cgatactggt
     6301 atattctctt ttctggattg cattttttat tgcagcaaga ctgttgttca ttattacaaa
     6361 gttccgggaa gcagttgaat tcagtccggc gcagctgttt tccaccttcc ggcatggcat
     6421 gaaactggat ctttctgcaa cagcatacat cctggtaatc ccggtactgc tcatgatccc
     6481 cggaattttt ctggaaggca ggtggttcag aagattcatg aggtattata cgattataat
     6541 taccggcatt tcctccttaa ttgttgtatc tgataccctt ctttacaaat actggggttt
     6601 ccggatggat cacacccctc ttctatatct ccggacaccc ggagctgcaa tggcatccgt
     6661 aagcacattc cagattatcg ctgttgcagc agggataata cttctggcac tgttgttcat
     6721 tttcctctac tacagatttg ttgaaaggta ttttgagata tccgggaaaa tccgtttcag
     6781 gtttcctgcc ctgctctttt ttgttatatg ctggggatcc cttcttatcc ccatacgggg
     6841 aggagtgggt atagctccga taaatgcggg aacggtttat ttcagcaaag atatgtttct
     6901 caatcattca gcaatcaatg ttgtctggaa tgtggggaac tctctggcca ataataaacc
     6961 ggtaagcaat ccctatcagt tttacgatac ctcagttgca aggcagctga ccgcttccct
     7021 cacggctgac agcggatcgc ccatgattct tgtaaatgat tcaagaccga atatcctgat
     7081 ttttgtgctg gagagtttcg gaagtgcaat gatcgggcct ttgggcggag atccccttac
     7141 cacaccgaac ttcaatcgtt acgtgaagga tggcatcctt tttacccgct tttttgcatc
     7201 cggaaaccgg acagacaagg ccgttcctgc catactctgc ggctatcccg ctcagccggc
     7261 cacttcaata atgaaagagc cgaaaaaaac acaatccctt ccgggactgg taaaaatgat
     7321 gaacagcaag ggatatcaca cttctttctg gtacggcggg gaaataaatt ttgcaaattt
     7381 caattccttt gtcattaatg ccggattcat gagtgttata acacgggaca acttcagtcc
     7441 cgggttttat aattcgaaat ggggtgtcca tgatcatgta cttcttgaag ccctgcagga
     7501 ttccatgaaa aacataaggg agccttttct gaaggtggtt cttacccttt cgagccatga
     7561 acccttcgat gtgccgatga cgcctgtatt caggggaaat gatgacctta ccaggttccg
     7621 gaactcaatt tactataccg acaggtccct ggggcagttt ctggaccagg caaaaaccac
     7681 ggagtggtgg aaaaat
//
LOCUS       DAOSPX010000145         7657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_8984_length_7657_cov_3.599184, whole genome shotgun sequence.
ACCESSION   DAOSPX010000145 DAOSPX010000000
VERSION     DAOSPX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8984_length_7657_cov_3.599184"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="asnA"
                     /locus_tag="PLZ75_08190"
     CDS             complement(<1..971)
                     /gene="asnA"
                     /locus_tag="PLZ75_08190"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOS72410.1"
                     /translation="MTHQAETIEVSCANDMLLTEEMIDFIKTRYEKLLAGELNLVKVS
                     APVVVPDGTGINDDLNGVEEPISFPVKSLNGQKASIIQSLAKWKRMRLRYYHIAPGKG
                     ILTDMRALRPSEILTPIHSVYVDQWDWEKHIEPGERSLKLLKSTVASIYKTFRQIEKE
                     LCEKYPVNTPQLPEEIKFIHAQDLLDEYPDLSPKQREAKAAKKYGAVFIIGVGHVLSN
                     GEPHDGRAPDYDDWSTLNDDGYYGLNGDIIFWNPALESAFEISSMGIRVDRKSMERQL
                     EISGCQDRRDLLFHRLLLDGQLPLSIGGGIGQSRLCMFLLKKLHIGEV"
     gene            1384..2283
                     /locus_tag="PLZ75_08195"
     CDS             1384..2283
                     /locus_tag="PLZ75_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72411.1"
                     /translation="MKLKTLLFSGALFTFAAINAQKPIVIQEDSLNLGANRYPAISVL
                     IPEVKYEKTRKNWIRKQETGTKSKVVDDAGKMSIFGAKSKNVSPDPFNILSMLADQDS
                     VLRLTAAFEIRKDVYIERSTGEAELAKAKTFLFDFAKDQYTDLVNDQLKQEQSKLKDL
                     EKELRSLEKRQSGMEKDIRTGNRQLVTEREKLTLLNNELTTSTTALAEHNAEYMVMEP
                     GDEKDAKARYIKDLEKQKRSTVRSIKKSEKRIRNAESKISRANRAIPKNDSTQERIRR
                     LINDQENVVQLFSDKLSKVKAFK"
     gene            complement(2405..4579)
                     /locus_tag="PLZ75_08200"
     CDS             complement(2405..4579)
                     /locus_tag="PLZ75_08200"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOS72412.1"
                     /translation="MKKIFVLIILVQAFFPCRQANAFPDEGMWIPALIEKFNISIMKE
                     KGFRLSAEDVYSVNRACMKDAVLSFGGGCTGAVISPDGLLITNHHCGYGQIQRLSTVE
                     KDYLANGFWAMSRDEELQCPGLFVTFLKRMEDVTGEMMKGISPDMDNEARDILLDSNS
                     EKIRKDAVEGTHYTASVRPFYMGNQYFLLVYETYNDVRLVGAPPSAIGKFGGESDNWT
                     WPRHTGDFALFRIYAGKNNEPAAYSPDNVPFQSRYHFPVSLRGIREGDFTMIFGYPGT
                     TMQYVPSYHIDMVKNYINPAMIKIRDRKIEIMETAMNTSPKVRLQYSAKKSGIANGWK
                     KWIGENQGLDKMQTISRKQEYEKQLTEWIGADDGRREKYGHILPRYAEIYPELKKYSL
                     VNSITNDAFFNSGIEAVGLASNLSAFAGMYEGSMDPEKRESYRNAMLAYAGDFFRNYD
                     RATDMELFVSVMQLYGEILEPEWQAPEYIRLKKLCGGDFRSLAGRIYDRTIFADQDRL
                     TSFIRDFKRSGARELKKDPFYQLAESASLLISERVRDRLGRLNAEIRELNRLYMALQM
                     EYNRDQVFYPDANSTLRIAYGTVSGYKSADAVYFSHLTTLKGIMEKDNPEIYDYDVPA
                     GLRQLYEEGDYGRYSQDGQLPVCFIASNHTTGGNSGSPVINADGHLAGVNFDRAWEGV
                     ASDMAFNPEQSRNISLDIRFALFIIDKFAGAGYLLDEMTLVD"
     gene            complement(4763..5359)
                     /locus_tag="PLZ75_08205"
     CDS             complement(4763..5359)
                     /locus_tag="PLZ75_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOS72413.1"
                     /translation="MLGYELKRYRIILASRSLRRRQLLEELGLEFTVVEKDYDETFPD
                     NLSGEATALWLAGRKAALFRNSLLPDEIVITADTVVWCGGRILDKPSGEEEAREMIRI
                     LSGKTHEVITGVNLMAKSAERIFSDTTRVTFGILSEEEISYYVENFRPYDKAGAYGIQ
                     EWIGLVACSRIEGSYFNVVGLPVHLIYRELRELIKNLN"
     gene            complement(5363..6364)
                     /locus_tag="PLZ75_08210"
     CDS             complement(5363..6364)
                     /locus_tag="PLZ75_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOS72414.1"
                     /translation="MHLPGDIRYVLRLIRWQNLLIVILTMVLMRYALVQTLAGGIYVM
                     LDEGQGEAVRMTLRFPWYDFVILVFATVCITAGGYVINDYFDIRTDLINRGEVIIGTR
                     IPRRKAILWHSILNIAGVTAGFYVSARAGYLWFGTIFLLISGLLYFYSASYKRQFLVG
                     NLLVAVLTALVPVLAVLFEWTGLYRYYSVHAGNLPDLKFLFWWAGGFALFAFLTTFIR
                     EIIKDIEDFEGDAAYGRNTLPVVTGIPVSKFISVSLIIATIVLLFLAWFLFISDMITL
                     IYLSAAIVLPLVAAVFRLLSGKNRKELHAASGLIKLVMLAGILYSLVVKIIIDCNLL"
     gene            complement(6348..6869)
                     /locus_tag="PLZ75_08215"
     CDS             complement(6348..6869)
                     /locus_tag="PLZ75_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOS72415.1"
                     /translation="MDNFREGLTRIKAFVFDIDGVLSLQTIPLNTSGIPSRTVNLRDG
                     YALQLAVKKGYKVAIISGSNSRDYLKRLKMLGIKDIYLNSRSKLRHYNHFREKYGLEN
                     SEILFMGDDIPDYQVMKMAGVPACPSDADSEIKHVAMYISDRAGGAGCVRDVIEQVLR
                     LHNNWMDSDAFAW"
     gene            complement(6990..7403)
                     /locus_tag="PLZ75_08220"
     CDS             complement(6990..7403)
                     /locus_tag="PLZ75_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72416.1"
                     /translation="MKLYIFRKIVVAMIIILPVFSWTGCKKQPKCGCDGDVIRTINNE
                     LMDRSRIIYSQDGSSAYFTIANGIYYDTYHFCNPGEMFPKFRELAGEDQIIISGNLFW
                     ECTYMMNSSNYSYYSYYYKVYNIQVTELKSYLYGK"
BASE COUNT         2090 a         1910 c         1642 g         2015 t
ORIGIN      
        1 acctctccga tatgaagctt tttcagaagg aacatacaca gtcgtgactg gccgataccg
       61 ccgcctatac tcagcggcag ctgaccgtcc agcagcagtc tgtggaaaag gagatccctg
      121 cggtcctggc agccgctgat ctctagctgt ctttccatag atttcctgtc aaccctgata
      181 cccatggatg atatctcgaa agccgactcc agggcaggat tccagaaaat gatatcgcca
      241 ttgagcccgt aatagccgtc gtcgttaagg gtgctccagt cgtcataatc gggcgccctt
      301 ccgtcatgag gttccccgtt gctcagcaca tgaccgacac caatgatgaa taccgcacca
      361 tatttttttg ctgcttttgc ctcacgctgc ttcggactga gatccgggta ctcatccagc
      421 aggtcctggg catggatgaa ctttatttcc tcaggcaact ggggggtgtt gacgggatat
      481 ttttcacaca actccttctc tatctgccgg aatgttttat agattgacgc aaccgtggat
      541 ttaagcagtt tcaatgaccg ttccccgggt tcgatatgct tctcccagtc ccactggtca
      601 acatacacgg aatgaattgg cgtaaggatt tcacttggcc tgagagcgcg catatcggta
      661 agaatgcctt ttccgggagc tatgtgataa tatctcagac gcatcctttt ccactttgcc
      721 agggattgta ttattgaggc tttctgaccg ttaagggatt taacgggaaa gcttatgggt
      781 tcttcaaccc cgttaaggtc atcatttatt cctgttccgt caggtaccac aaccggcgcc
      841 gaaaccttca caagattcag ctcacccgcc agaagctttt cataccgcgt cttgatgaaa
      901 tcaatcatct cctcggtcag cagcatatcg tttgcacatg atacctcaat cgtctctgcc
      961 tgatgtgtca taactgctca atgttttaca atcgcaaata gcattttata ttttgaagca
     1021 aatatgcata tatattcata tttttcaaaa tttctcgtca aatatattat aatatattat
     1081 ttattcatct ctatttataa caaaccaaat gtacggaaat ttccggctgt ttgcacaagc
     1141 aagaatgatt ttttatattt gacggcgttt caaaaaccct gataaagcat aacagaccaa
     1201 taagtaagag gtcccgttca ggcagaataa acaaaaacca gaatagcttg ttaaacacgt
     1261 ggattctccg gattggcaga aaaccaagag atttttaaat ttaaattatt gaatacagga
     1321 tacgaatgtt ctcctgctta taagacaaga ctgtccggaa attaaaacta aaaacacaat
     1381 tatatgaaac ttaaaacttt actcttttca ggcgcgctgt tcacatttgc cgccatcaat
     1441 gcccagaaac cgatagtgat tcaggaagac agcctgaatc tcggggcaaa cagatatcct
     1501 gcaatttcag tacttatccc ggaggttaaa tatgaaaaaa cccggaagaa ctggataagg
     1561 aaacaggaaa ccggcacgaa gtccaaggtt gtggatgatg ccggcaagat gtcgattttt
     1621 ggtgcaaaat ccaaaaacgt ttccccggat cccttcaaca ttttaagcat gctggccgac
     1681 caggattcgg ttctcaggct cacagccgca tttgaaatcc gcaaggatgt ctatattgaa
     1741 agaagcacgg gagaagctga gctggcaaag gccaagactt ttcttttcga tttcgcaaag
     1801 gatcagtata ccgatctggt aaacgatcag ctgaaacagg aacagagcaa gctgaaagat
     1861 ctggaaaagg aactgagatc acttgaaaag cggcagtcgg gaatggaaaa ggacatccgg
     1921 accggaaaca ggcagctggt gacagaaagg gaaaaactga ccctcctcaa caatgagctt
     1981 acaacttcca ctactgccct tgcagaacat aatgcggaat acatggtaat ggaaccggga
     2041 gatgagaagg atgcaaaagc caggtatatc aaggaccttg agaagcagaa aagatcaact
     2101 gtgagatcta taaaaaagtc tgaaaaaaga atcagaaatg ccgaaagcaa gatcagccgt
     2161 gcaaacaggg ccattccgaa aaatgacagc acacaggaga gaataaggcg gctgatcaat
     2221 gaccaggaaa acgttgttca gctgttttca gacaaactta gcaaagtaaa ggctttcaag
     2281 tgattgccag aaacactgag tgctgatatt tacccgggat ttataccgga aacggcactg
     2341 agcggaactt cattccgccg gtgccgtttt ttgtattacc ggaaggagag ccatgaggca
     2401 ggcatcagtc aacaagcgtc atctcatcaa ggaggtagcc tgcaccggca aatttatcta
     2461 tgatgaacag ggcaaatctg atatccagac ttatattcct gctttgttcc ggattgaatg
     2521 ccatgtcact ggcaactccc tcccacgccc ggtcaaaatt cactcctgcc agatggccgt
     2581 ctgcattgat aaccggactg ccggaattgc ctccggtggt atgattgctg gcgatgaaac
     2641 acaccggcaa ctgaccgtcc tgtgaatatc tgccataatc accctcttca taaagctgtc
     2701 tgagcccggc aggaacatcg taatcataga tttcgggatt atccttttcc attattccct
     2761 tcagtgttgt aagatggctg aagtatacgg catccgcgga cttatatccg cttactgttc
     2821 cgtaagctat tctaagtgtg gagtttgcat caggataaaa aacctggtcc ctgttgtatt
     2881 ccatctgcag ggccatataa agcctgttga gctcccttat ttcagcgtta aggcggccca
     2941 gcctgtcgcg aaccctttcg gaaatcagaa gggatgcgct ttccgcaagc tggtaaaacg
     3001 gatccttctt cagttcccgg gcacccgatc tcttgaaatc tcttataaag gatgtcagcc
     3061 ggtcctgatc ggcaaagatg gtcctgtcat aaatccttcc ggcaagggaa cggaaatctc
     3121 ctccgcacag ctttttgagc cttatgtatt ccggagcctg ccattccggt tcaagaatct
     3181 cgccataaag ctgcatcacc gaaacaaaca gctccatgtc cgttgcccgg tcatagtttc
     3241 tgaaaaaatc cccggcatag gccagcatcg cattcctgta gctttccctc ttctccggat
     3301 ccatgctgcc ttcatacatc ccggcaaagg cagacaggtt tgaggccagt ccgacagcct
     3361 ctatcccgct gttgaaaaat gcgtcattgg taatactgtt taccaggctg tattttttca
     3421 gttcaggata aatttccgca tagcggggaa ggatatggcc gtatttttcc ctgcgtccgt
     3481 catctgcacc gatccattcc gtcagctgtt tctcgtattc ctgtttcctg ctgatagtct
     3541 gcattttgtc cagtccctgg ttttccccta tccatttttt ccagccattg gctatcccgg
     3601 atttctttgc agaatactgc agccttacct tcggactcgt attcatggca gtttccataa
     3661 tttctatctt cctatcccgg atttttatca ttgccgggtt aatgtagttc ttgaccatat
     3721 caatatggta tgacggaaca tattgcattg ttgttccggg atagccgaaa atcatggtga
     3781 aatcgccctc cctgattccc ctgagggaga ccgggaaatg ataccttgac tgaaaaggga
     3841 cattatccgg ggaatatgca gctggttcat tgttttttcc ggcatagatg cggaaaaggg
     3901 caaagtcccc ggtatgacgc ggccatgtcc agttatccga ctcccccccg aatttgccta
     3961 ttgcggaagg cggggctcct acaaggcgga catcattgta tgtttcataa acaagaagga
     4021 aatactggtt gcccatataa aacggcctga cacttgcggt ataatgtgtg ccttcaacgg
     4081 catccttccg gatcttttca gaatttgaat ccagcagaat gtcgcgggcc tcattatcca
     4141 tatcaggact tatccccttc atcatctcac cagtcacatc ctccatcctt ttgaggaaag
     4201 tgacaaaaag tcccggacac tgcagttcct cgtcacgtga cattgcccag aaaccatttg
     4261 caagataatc cttctcaact gtcgacagcc gttgtatctg tccgtagccg caatgatgat
     4321 tggtaatgag cagtccgtcg ggtgatatga cggcacctgt gcatcccccg ccgaaagaaa
     4381 gcaccgcatc cttcatgcag gccctgttga cgctgtacac atcctctgcg gaaagcctga
     4441 atcccttttc cttcatgatg ctgatattga atttttcaat aagggcaggg atccacatcc
     4501 cttcatctgg aaaggcattt gcctgcctgc aggggaaaaa ggcctgaaca aggattatta
     4561 atacaaaaat ttttttcatg cttattttca tttccggatt taacagtatt ttcaaaaata
     4621 ttaatacaaa cctgactgat actgcagaat aaattaaagc ctgacaagta aacccaattt
     4681 gctcaaacca cactgacacc actgcccgct tgattcatac cacatgttct gaatgcggat
     4741 aagccagggc caccggggcg gatcagttca gattttttat cagttcccgg agttccctgt
     4801 agatcaaatg gacaggaaga ccgacaacat tgaaatatga accctctatc cggctgcatg
     4861 caaccaggcc tatccattcc tggatgccat aggcaccggc tttgtcgtag ggtctgaaat
     4921 tttcaacgta ataacttatt tcctcttccg acagaatacc gaaggtcacc ctggtggtat
     4981 cgctgaaaat cctttctgca gattttgcca taaggttcac tcccgtaatg acctcatggg
     5041 tctttcctga aaggattctg atcatctcac gggcctcctc ctcccccgac ggcttgtcca
     5101 ggatcctgcc gccgcaccat accacggtgt cggctgtaat aacgatttca tccggcagta
     5161 gactgttccg gaacagggcc gctttccggc cggccagcca gagcgccgtg gcttctcccg
     5221 aaagattgtc aggaaatgtt tcatcatagt ctttttcaac caccgtaaac tcaagtccga
     5281 gttcctccag cagctgcctc ctcctcaatg atcttgaagc cagaataatc ctgtatctct
     5341 tcagctcata gcccagcata tgtcataaaa ggttacagtc aatgattatc ttcaccacca
     5401 atgaataaag tattcctgcc agcattacca gcttaatcag tccggaagct gcatgaagct
     5461 cctttctgtt tttcccggaa agcagtctga acacagctgc caccagggga agcacaatgg
     5521 ctgccgacag atagatcagg gtgatcatat cactgataaa caggaaccag gccagaaaca
     5581 ataatacaat ggtggcaatg atcaggctga ccgaaataaa tttggaaacc ggaattcccg
     5641 tcacaaccgg cagggtattc cttccatagg ccgcatcgcc ttcaaaatcc tctatatcct
     5701 tgattatttc ccgtatgaag gttgtaagaa aggcaaacag ggcaaaccct ccggcccacc
     5761 agaaaagaaa tttcaggtcc ggcagattgc ctgcatgaac ggaataatat ctgtatagtc
     5821 cggtccattc aaaaagtaca gcgagaaccg gaaccagggc cgtcagcacg gcaaccagca
     5881 gattgcctac cagaaactgg cgcttgtagc tggccgaata aaaatagagc aggcctgaaa
     5941 taagaaggaa aatggttccg aaccagaggt atccggccct ggcagaaacg taaaatccgg
     6001 cagtaacgcc tgctatgttc aggattgaat gccagagaat ggctttccgc ctggggattc
     6061 ttgttccgat aatcacctct cccctgttga taaggtctgt cctgatatcg aaataatcat
     6121 ttattacata gcccccggca gtgatacaga cagtggcaaa taccagtatc acgaaatcat
     6181 accacggaaa acgaagcgtc atcctcaccg cttccccctg tccttcatcc agcatgacat
     6241 atattccccc tgcaagtgtt tgaacaaggg cgtatctcat cagcaccatg gtaaggatca
     6301 ctataagcag attctgccac cttatcagac ggaggacata tcttatatca ccaggcaaat
     6361 gcatcagaat ccatccagtt gttatgtaat cggagaacct gttcaataac atccctgaca
     6421 catccggcac ctccggccct gtcggaaata tacattgcaa catgttttat ttcgctgtcg
     6481 gcatctgaag gacaggccgg cacacctgcc attttcatca cctgataatc gggtatgtca
     6541 tctcccatga atagtatctc ggaattttcc aggccgtact tctccctgaa atgattgtaa
     6601 tgacgtaatt ttgaacggct gttaagataa atatccttta tcccgagcat cttcagtctt
     6661 ttcagatagt ctcttgaatt gctgcccgag attatggcta ccttgtatcc ctttttcacc
     6721 gccagctgca gggcatagcc atccctgaga ttaaccgtcc tgcttggaat acctgatgtg
     6781 ttcagtggta tagtctgaag agacaatact ccgtcaatat cgaaaacaaa tgccttgatt
     6841 cttgtcagtc cttccctgaa attgtccatt taaggtattg tattatgaaa aatgatttcc
     6901 ggttcaggaa tgccgtcatg caggtctgat ttctgcaact gcgttttatg tacgggatga
     6961 aaatccgttc catgctgtga aaatccccgc tatttaccgt aaagatagga tttcaactcg
     7021 gtcacctgga tattgtagac cttataataa taggaatagt aggaataatt gcttgaattc
     7081 atcatgtatg tacattccca gaacaggttg cccgatatta ttatctggtc ttcaccggcg
     7141 agttccctga atttcggaaa catttccccg ggattgcaga aatgataggt atcatagtaa
     7201 ataccattgg ctatggtgaa ataggctgag cttccgtcct ggctgtagat tatcctcgag
     7261 cggtccataa gttcattgtt gattgtacgg ataacgtccc cgtcacagcc gcatttgggc
     7321 tgttttttgc atcccgtcca tgagaatacc ggaaggatga tgatcattgc caccactatc
     7381 ttccggaaaa tatagagttt catctcagaa taaactgttt agattgttaa ttataaacct
     7441 gcaaaataag attttatttc gtgcattaca aaaaaaacaa gtcgccagca ctgatctgac
     7501 gaccctgaca atgcagccgg aactatttac cggctgcctt catggcagta actgaacgcc
     7561 aattccgccg gcggcgaact catgcccgga ctgccggaat gcggtctcaa gcctgggccg
     7621 tcggccctcc gaagctcagc ggcataacgg gacccga
//
LOCUS       DAOSPX010000146         7641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9004_length_7641_cov_4.400211, whole genome shotgun sequence.
ACCESSION   DAOSPX010000146 DAOSPX010000000
VERSION     DAOSPX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9004_length_7641_cov_4.400211"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLZ75_08225"
     CDS             <1..218
                     /locus_tag="PLZ75_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72417.1"
                     /translation="DLSDLFTAVPDFAPYRALPPDLRIFDPEKAFTPLDEEFDWDYLS
                     KSPVLDDPDEMVKFQKEKPEYRLEDQK"
     gene            complement(286..1854)
                     /locus_tag="PLZ75_08230"
     CDS             complement(286..1854)
                     /locus_tag="PLZ75_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72418.1"
                     /translation="MKHIALLLFLFQFPVISSLSQVKSGNPSDNTLKIPEFVSYRDFG
                     AIGDGKTDDTEAIAAAHEYANQHGLAVKAGDSAVYYISGKNLPVIIRTDTDFGNASFI
                     IDDREVQNRNADVFMVLSALQPFRPEGISSLKKNQEKIDIPFPGTCLVTVSNSGVRRY
                     IRFGPNQNSGSAQTDIFIVDKDGRVDMNSPIIWDFDHITDIRAIPVDETTLTISGGRF
                     TTIANSEESRYNYYSRGIAIRRSNVVVKDLEHRITGEGEHGAPYGGFINISDCAFVTV
                     RNVILTGHKTYQTIGSAGVSVSMGSYDILATRALNVSFIGCSQTNDINDGRYWGILGS
                     NYCKNLVYDNCTFSRFDAHMGVVNATIRNSVLGHQGINAIGSGIFTVENSTIYGRNII
                     NLRSDYGSTWQGEFIIRNCTFVPAGGRPVSASLIGGFWSGQHDFGYTCYMPERIIIEN
                     LHIDDSGHPENYDGPAIFADFNPEMTDDSRTETHPYIRTREVILRNVSTASGKNLRIS
                     DNPYMFREVRIKSE"
     gene            2183..3523
                     /gene="mgtE"
                     /locus_tag="PLZ75_08235"
     CDS             2183..3523
                     /gene="mgtE"
                     /locus_tag="PLZ75_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOS72419.1"
                     /translation="MLERIKEIEELLENKEWKILRSKISDLEDLDIAETIEELSSGDD
                     VLIFRLLSKTQAKNTFQLLSYEKQEQVIEGLARNSTKLSALLNDLDPDDRTAFFEELP
                     GEVSQKLIQLLSPEERKIAVQLLGYPEDSIGRLMTPEFVAVKSHFTVRQTLSHIRVYG
                     HNSETLNVIYVVDDKWKLIDDIKIREIILANPDQNIMELLDSRYVSLNAYDDQESAIR
                     VFKDHDRVALPVTDSEGILLGIVTFDDIMDIEEEETTEDFHKFGSVQDAIIDPLKARV
                     PLLYQKRIIWLMILVFMNVFSGAAIASFEELIKTMVPLMFFLPLLIDSGGNAGSQSAT
                     LMIRSLAVGDVEMRHWFKLVGKEFVVSFLLGITMAAGVSLIASFRAPDTILVVSLSML
                     LVVMTGSLLGLTIPFLFTKLKLDPATASAPLITSIADIMGVWIYFSVAAWVFGY"
     gene            complement(3570..4331)
                     /locus_tag="PLZ75_08240"
     CDS             complement(3570..4331)
                     /locus_tag="PLZ75_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72420.1"
                     /translation="MAVLLFSSCTKENDMANVQVKFRTVQSSAKIARGEMSSLPETEY
                     TSGSSLSGTGYPSGASFVPDKFLINIKEIEFEFEKPYPGFTGPHNCECEKDGECEIEF
                     EGPFLIDIASSEAMTGLLLGRFPLPSAVYEEIELDIAPSRNRNNKQINGRSVFISGTY
                     QGKPIELWTNSKAELEIEFPGKRAVDLTQPNADMWIRIHLDRIIANLSAMDFSSAVDG
                     NGNGIIEIGHDDQDGNNALSATLFNSFRGSFELDD"
     gene            complement(4540..5526)
                     /locus_tag="PLZ75_08245"
     CDS             complement(4540..5526)
                     /locus_tag="PLZ75_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72421.1"
                     /translation="MKQKVKNLIFVIPLVFLSSCATLMLKKDYNIRVSANVPDAKAEI
                     QDSIYSLPAVFRIKRSKEDLNIRLISDSLERNYIVKASPNAAFLYANLICYPVLPLMY
                     GIDFTNQKRFYYGKNIILNSCDTTSIIRPPVAKSYYDYRTRSFPSNKGQLNFTYSFPW
                     VNSFFLQPQHESSKSNTGFWGFSAGMEYYYKDNKYLSLNGSAVTDFFIPFPAAVDFSG
                     EVENMYSVYASLTDNYKFRRFTLGYGLSFSRNTWELVYHDRFDPPPPTRDPARKSGSS
                     VGLILDGYHQIGRHFYIGLIYRPTFLNINPDFKYRYEHLISMDFKVKLRLMK"
     gene            complement(5710..6006)
                     /locus_tag="PLZ75_08250"
     CDS             complement(5710..6006)
                     /locus_tag="PLZ75_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72422.1"
                     /translation="MDSGTIKVQNKVHNAKLENISFGDVSLYSSLLPGETSSKKVITD
                     KKESFPKINQLEFYMAGSGNRVYLKTKTKYKLDAGESLLIVISDSTAVINPLNQ"
     gene            complement(6061..6633)
                     /locus_tag="PLZ75_08255"
     CDS             complement(6061..6633)
                     /locus_tag="PLZ75_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72423.1"
                     /translation="MKKLIFFIMISPLLIKCSKEDYSPYFYSNESTIFSDSKTTKFRE
                     FVIIIVPYVLTGDEKKYIVTDTIKNVRIKVNNQLWGIYHSFAIDTSIFVKEKLINFYS
                     TRTKTKYSIIAPYKTSLDTLKTAGAYSDLLNNYLAMEPGNYICQLESFDIKQIDGKKR
                     TVKPFIVFPVEVRENSRSALAGEFEVQVNN"
     gene            complement(6720..>7641)
                     /locus_tag="PLZ75_08260"
     CDS             complement(6720..>7641)
                     /locus_tag="PLZ75_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72424.1"
                     /translation="TVPVSPETATDPSSALTVSPAGGTAVSGEAIQFHDGTALPPGRT
                     AGGIPETTESEKTDRVTDISRISGTAGMETIIAGLRETVTGDFTGLVASVIETTAPPK
                     KSSGPFIRFQDAGLMYSYKNTWLLNYETRNGLNPSRLGNTLPTFHHDLGITSTIVFRE
                     KHDIGLEVLLMSETGQNYQQYINASFVNRKITLNYFKLQTWYMWNHRKIPGSTIAGVY
                     FARLGMAEEIQGDLRFSVRDDYRNYDYGLVLGYRLDVPLFSNIVMYPGIRFNYNLMNI
                     FEGHAGTMNIFKTTNNFTAGVNVTFAYSFR"
BASE COUNT         2002 a         1609 c         1698 g         2332 t
ORIGIN      
        1 cagatctttc agacctgttt accgccgttc cggattttgc tccctacagg gcgttgcctc
       61 ctgatctgag gatctttgat cccgaaaagg cattcacacc gcttgatgag gaatttgact
      121 gggattatct gagtaaatca ccagtgcttg atgatccgga tgaaatggta aagtttcaga
      181 aggaaaagcc cgagtacagg ctggaagatc agaagtaaag ttgtttaaca ggtttctccc
      241 cggtgatata ccaggcttca cgctgctttc ccggccccgg gataattatt cagattttat
      301 tctgacctcc ctgaacatat agggattatc actgattctc agattttttc cgctggcagt
      361 acttacgttt ctgaggatga cttccctggt tcttatataa ggatgggttt ccgtccggga
      421 atcgtctgtc atttccgggt tgaagtcagc aaatatggca ggaccatcat aattctcagg
      481 atgcccggag tcatcgatat gaaggttttc gattatgatt ctttccggca tgtaacaggt
      541 gtagccgaaa tcatgctgtc ctgaccagaa tcctccgatc aggctggcac tgaccggtct
      601 gcctccggcc ggcacgaatg tgcagttgcg gataatgaat tcaccctgcc atgtactgcc
      661 gtagtcggac cggaggttaa ttatgttcct tccgtatatc gtcgaatttt ccacggtgaa
      721 aattccgctt ccgattgcat ttattccctg atgtccgagg acggagtttc tgatggtggc
      781 atttacaact cccatatggg catcaaagcg ggaaaaggta cagttgtcat agacaaggtt
      841 tttgcagtaa tttgatccca ggatgcccca gtacctgccg tcgttaatgt cgttcgtctg
      901 gctgcagcct atgaatgata cgttgagggc cctggttgcc agaatgtcgt agctgcccat
      961 ggagacactt acccctgccg agcctatggt ctggtatgtt ttatgtccgg taagaatcac
     1021 attcctcaca gtaacaaagg cacagtcgct gatgttgata aaaccgccat aaggcgctcc
     1081 gtgctcccct tcgccggtta tccggtgttc cagatccttt acaacaacat tcgaccgcct
     1141 gatggcaata ccccggctgt agtagttgta cctggattct tcgctgttgg ctatggtggt
     1201 gaaacgtccg ccgctaatgg taagggttgt ttcatccacc gggattgccc tgatatcggt
     1261 tatgtggtcg aaatcccaga taatggggga attcatatcc accctgccat ccttgtcaac
     1321 aataaaaata tccgtctgtg ccgatccgct gttctggttg ggtccaaagc gtatatagcg
     1381 tcttactccg gaattgctga ccgtgacaag gcaggttccg ggaaatggga tatcaatttt
     1441 ttcctgattc tttttaaggg aggatattcc ttcgggcctg aagggctgaa gtgcagaaag
     1501 aaccatgaat acatctgcat tacgattctg aacttccctg tcgtcaatta taaaggaagc
     1561 attaccgaag tcagtgtctg tccggatgat tacaggaagg ttttttccgc tgatataata
     1621 gacggcgctg tccccggcct taactgccag tccgtgctga ttggcatatt catgagccgc
     1681 agcgatagcc tctgtatcat cagttttgcc atcgccaatg gctccgaagt cgcggtagct
     1741 gacaaattcc gggattttca gtgtattatc agaagggttt ccggatttta cctgtgacag
     1801 agaggatatc accgggaatt ggaacaggaa aagaagaagt gcgatatgtt tcatcaggtc
     1861 atattttaca gaaaggttct ggttaaaatt acaaattttg aaggatgaat ccggaagaaa
     1921 aactgcctga tattttattt atcgtaaatt accgcgggtt ccgggctttc gttacagatc
     1981 accaggatcc aggatgtaag acctaaagta aatacacaat aaaaaaggca tagattggtt
     2041 ttctgtgcta aaaaaaatac ttttgcagat ttatcgcccg gatgccggaa agattgtctg
     2101 ttccatggca acaagcggca gtgtatatct caggggcctt atcatgttgt acttgcaggt
     2161 taatgcttta gactgatgtg ctatgttaga aagaatcaag gaaatagagg aacttctgga
     2221 aaataaggag tggaaaatcc tgcggagcaa aatatctgat ctggaagatc tggatattgc
     2281 ggaaacaatt gaagagctgt cttccggtga cgatgtgctc atattccgcc tgttgtccaa
     2341 gacacaggca aaaaatacct ttcagcttct ttcttatgaa aagcaggaac aggtcattga
     2401 aggtctggcc aggaattcaa ccaaattgtc tgccctgctc aatgatctgg atcctgacga
     2461 cagaaccgcg ttttttgagg aactgcccgg agaggtttcc cagaagctta ttcagcttct
     2521 ttcaccggag gaacgcaaga tagctgttca gcttctgggc tatcccgagg acagcatcgg
     2581 aaggcttatg actcctgaat ttgtagctgt caaatcccat tttacggttc gtcagactct
     2641 cagccatatc cgggtttacg gtcataattc agaaaccctc aatgttattt atgtggttga
     2701 tgacaagtgg aaattaatag atgacatcaa gatacgggag ataatacttg ccaatccgga
     2761 ccagaatata atggaactcc tggacagtcg ttacgtttca ctcaatgcat atgatgatca
     2821 ggaatccgcc atccgtgtat tcaaggacca cgaccgtgtt gctctgcctg tcactgattc
     2881 tgaagggatt ttgcttggga ttgtgacttt cgatgacata atggacattg aggaagagga
     2941 aacaactgag gacttccaca aattcggttc tgttcaggat gcaattattg atcctctcaa
     3001 ggccagggta ccgctcctgt atcagaagcg gataatttgg ctgatgatac ttgtgttcat
     3061 gaatgttttt tcgggtgcgg ccatagccag ttttgaggag ctgataaaga ccatggtgcc
     3121 gctgatgttt ttcctgccgc ttctgatcga cagcggcgga aatgcaggtt cccagtctgc
     3181 tactctaatg atccgctccc tggcagtcgg agatgttgaa atgcggcatt ggttcaagct
     3241 ggtgggcaag gagtttgttg tgtccttcct gctcgggatc acgatggctg ccggtgtatc
     3301 cctgattgcc agcttcaggg ctccagatac aatacttgtg gtttcccttt caatgcttct
     3361 tgtggtaatg acaggaagtt tactcggtct taccatacca ttccttttta caaaactgaa
     3421 acttgaccct gccacggcca gtgcaccgct tatcacatca atagcggaca ttatgggtgt
     3481 atggatatat ttttccgttg cagcctgggt tttcggctac tgaagaaact acccggcaga
     3541 tgcctcataa taggcacgga tctccggtct tagtcatcca gctcaaagct gcccctgaag
     3601 gaattgaaca atgtggcaga cagtgcatta tttccatcct ggtcatcatg acctatttca
     3661 atgataccat ttccgttacc gtcaacagca gaggagaagt ccattgctga gaggtttgcg
     3721 atgatcctgt ccagatggat cctgatccac atatcggcat ttggctgggt aagatcaact
     3781 gcccttttcc ctggaaactc aatttcaagt tcagctttgc tgttcgtcca taattctatg
     3841 ggttttccct gataggtacc gctgataaat actgaccgtc cgttaatctg cttattgttc
     3901 ctgttacggg agggagcaat atcgagctca atctcctcat atacggcaga ggggagggga
     3961 aacctgccga gaagcagacc ggtcatggcc tctgacgaag caatgtctat gaggaaaggg
     4021 ccttcaaatt caatttcaca ttctccgtct ttttcgcatt cacagttatg cggcccggtg
     4081 aatccgggat atggtttttc aaactcaaat tcgatttcct ttatgtttat cagaaattta
     4141 tccggaacaa atgatgctcc ggagggatat cctgttccgg acaaggatga gccggatgta
     4201 tattctgttt caggcaatga tgacatttcg ccacgggcaa ttttcgcact tgattgtaca
     4261 gtcctgaatt taacctgaac atttgccatg tcattttctt tcgtacagga agaaaaaagt
     4321 aaaactgcca ttccgaccaa acttaaaaaa attgtgattc gtttcatttg tgtgtgtttt
     4381 gattaataaa atggttttaa aatttttctt tctgcctgta agacggagga atttgaaaag
     4441 gttggaaagg gaaggctttt ttcccggcag ccggcaggac tggatgcttg atttagccat
     4501 ttaatcccgc tgttttatac atgatgttgc cagcagtttt tatttcatta atcgaagttt
     4561 caccttaaaa tccatactta tcagatgttc atatctgtat ttaaaatcag gatttatatt
     4621 cagaaaagtt ggtctgtaaa ttaaaccaat ataaaagtgc ctgccgatct gatgataacc
     4681 atcaagtatc agccccacgg agctgcctga tttccttgcc ggatctcttg tcggcggcgg
     4741 cgggtcaaac ctgtcgtgat aaacaagttc ccaggtgttc cttgaaaaac ttaatccata
     4801 tccgagtgta aatcttctga acttataatt gtccgttaaa cttgcataga ctgaatacat
     4861 attttcaact tctccgctaa aatcaactgc cgccggaaaa ggaataaaaa agtctgttac
     4921 agcacttccg ttcaatgata aatacttatt atccttatag taatactcca ttccggctga
     4981 gaacccccaa aatccggtat tcgattttga tgattcatgt tgcggttgca ggaagaaact
     5041 gtttacccac ggaaaagagt aagtgaaatt taattgtccc ttgtttgacg ggaagcttct
     5101 ggtccggtaa tcataataac ttttggcaac agggggtctt attatacttg tagtgtcgca
     5161 ggaattcaga ataatgtttt tcccgtaata gaatcgtttc tgatttgtaa agtctatacc
     5221 gtacataagg ggcagaaccg gatagcatat caggtttgca tataaaaaag ctgcatttgg
     5281 cgaagcttta acaatatagt ttctttcaag gctgtcactt atcagcctta tattcaaatc
     5341 ctcttttgaa cgctttatcc ggaatactgc gggtaagcta taaatgctat cctgtatttc
     5401 agcttttgca tccggaacat tggctgacac tcttatatta taatcttttt taagcatcag
     5461 tgtcgcacat gatgaaagaa aaacaagcgg aatcacaaaa atcaggttct tcactttctg
     5521 tttcattttt tttgtcattt aatctgttga atccggatct tgctccaata gaaatcgcgg
     5581 ggtaagttat attatccgtg tattatgtta actgacgtgt catgggaagt gaaaggctga
     5641 tgtggtgcag aatgcctgtc ttgaccatca gtgtcccaat gttttatata caatgttggc
     5701 aatcgtggat tattgattta atggattgat tactgctgtt gaatcagaaa taactatcag
     5761 tagtgattct cctgcatcaa gtttatattt agtcttagtt tttaaataaa ctctattccc
     5821 actacctgcc atgtagaatt cgagttgatt tatttttgga aacgactcct ttttatcagt
     5881 aattaccttt ttggatgaag tctcacctgg tagcagggaa gagtaaagcg aaacatcacc
     5941 aaaacttata ttctccagct tcgcattatg tactttattt tgaactttta ttgtcccgct
     6001 gtccatgcat gctgtcatcg ttaagcatga aatggtaagt aatagtattg atctcataaa
     6061 ctaattatta acttgtactt caaattcacc agctaacgca ctccggctat tctccctgac
     6121 ttcgaccgga aatacaataa agggttttac tgtccttttt tttccatcga tttgtttaat
     6181 atcaaatgat tccagctgac atatataatt gccgggctcc atggccagat aattatttaa
     6241 caaatcagaa tacgctcctg ctgtttttaa agtatccagg cttgttttgt acggcgcaat
     6301 aattgaatat tttgtttttg tgcgagtgga ataaaaatta atcaacttct ctttcacaaa
     6361 tattgaagtg tcaattgcaa atgaatggta tataccccaa agttgattat ttacttttat
     6421 gcggacattt ttaatcgtat ccgtcactat gtattttttt tcgtctcctg tcagaacgta
     6481 gggaacaata atgatcacaa actcccgaaa cttagtagtc tttgaatctg aaaaaatagt
     6541 agattcattg gaatagaaat aaggtgaata gtcctctttg ctacatttta ttaacagagg
     6601 agaaatcata ataaaaaata taagtttttt cataggttat tgtgttagga tttttctttt
     6661 tatggttgtc agccaccttt aagacggaag aatttaaaaa ggttgtaacg gaacggtttt
     6721 taacggaagc tatatgcaaa cgtgacattt acgccggcag tgaagttgtt ggtggttttg
     6781 aaaatattca tcgtgccggc gtgaccctca aagatattca taaggttgta gttgaatctg
     6841 attccgggat acattactat gttactgaac agtggcacat ccaggcggta gcctaatacc
     6901 aggccgtagt catagttacg gtaatcgtcc ctgacgctga atctcaggtc tccctgaatt
     6961 tcctcagcca tgcccagtct ggcgaaataa acccctgcga tggtgctgcc gggaatcttc
     7021 cggtgattcc acatatacca tgtctgaagc ttgaaatagt tcagtgtgat cttcctgttt
     7081 acaaagcttg cattgatata ctgctggtaa ttctgtcccg tttcggacat cagcagaacc
     7141 tccagcccta tatcatgctt ttccctgaag acaatagttg atgttattcc caggtcatga
     7201 tggaaggttg gcagtgtatt gccaagcctt gagggattca ggccgttccg ggtttcatag
     7261 ttcagaagcc atgtgttctt gtagctgtac ataagtccgg catcctgaaa acggataaag
     7321 ggaccggatg attttttcgg cggggcagtg gtttcaatga cagaagccac aagtcccgtg
     7381 aaatcaccgg taactgtttc cctcaggccg gcaattattg tttccatgcc ggctgttccg
     7441 cttatccggg atatgtctgt tactctgtct gttttttcac tttctgttgt ttcagggatt
     7501 cctcctgctg ttctgccggg gggcaaggct gtcccgtcat gaaactgaat tgcttcaccg
     7561 gaaacagccg ttcctcctgc aggagatact gttaaagcgg aagaaggatc tgttgctgtt
     7621 tcaggtgaaa ccggaacggt t
//
LOCUS       DAOSPX010000147         7616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9037_length_7616_cov_4.622669, whole genome shotgun sequence.
ACCESSION   DAOSPX010000147 DAOSPX010000000
VERSION     DAOSPX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9037_length_7616_cov_4.622669"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLZ75_08265"
     CDS             complement(<1..572)
                     /locus_tag="PLZ75_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72425.1"
                     /translation="MTLWIIFISFILLALFLDLGVFNRSPHEIKTKEAAAWTAVWVSV
                     ALVFTGVVYFAFEKGWIDNPTGLSPSNAVLKYITGYIVELSLSVDNIFLIALIFSSFG
                     IPKKYQHEVLFYGVLGAVVFRAIMIIFGVVLINKLTWIIYVFGGFLIFTAVRMLMTHD
                     KKQDPKDSKIYKWLRKIYPVSRNLYDDKFFV"
     gene            complement(627..1295)
                     /locus_tag="PLZ75_08270"
     CDS             complement(627..1295)
                     /locus_tag="PLZ75_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72426.1"
                     /translation="MAWKLKAMAFSHLSVYKPLGSHAGINGGNSYYLNGVFPEASKIF
                     QMAGNSFTINENDPAGYVKREYPGADIQIEFSSCMGKTVLSGEYIFGTQPGGKGNSNP
                     DYSEISERDIYIRNFRRGYLNLIQDFVKSGLSAVTKFDFYDPNAKNPKKETGIGGTGK
                     GDIAWNTFGTGILCYINRDIRLTVYNDWLQNETSANLSGYDRGIADNVFTLRVQYSFY
                     KQLN"
     gene            complement(1525..1613)
                     /locus_tag="PLZ75_08275"
     tRNA            complement(1525..1613)
                     /locus_tag="PLZ75_08275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1577..1579),aa:Ser,seq:cga)
     gene            complement(1739..2521)
                     /locus_tag="PLZ75_08280"
     CDS             complement(1739..2521)
                     /locus_tag="PLZ75_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS72427.1"
                     /translation="MRSMLLTSGSAVILFLLCPGFFLKGQQVVFAEDFSGFTTGTHTT
                     PSTSDASGNLDSRTAVPGWTGSKVYSAGGEIKLGTASLTGWIETPPVSLSEEALILKF
                     DISRWPGDESTVQVYLNDLPLGNVITPTDDFQTSILSIPAETVTAKIRFAGLTKRFFL
                     DNIRIEQGNVSSSFNNSGEVQEKPLIYPNPASDFIRIENTMDFNRMEIADISGKTVRI
                     IDLSGRDNMVLSLESLPAGLYFIRFSSGKMICTSRLIIEKGR"
     gene            complement(2851..4089)
                     /locus_tag="PLZ75_08285"
     CDS             complement(2851..4089)
                     /locus_tag="PLZ75_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72428.1"
                     /translation="MKKIIIVLTISALVYSCGGRSGAENQKSKDMTTETANQYAWNRD
                     FLKKHVDIIELKRDNSAVALVPSWQGRVMTSTAEGENGFSFGWINHQLIESGIIQPHI
                     NAFGGEERLWLGPEGGQFSIFFRKGDDFVYDNWQTPSFLDTDPFEVVRLNDTSAFFAC
                     NAEAVNYSGTPLKFRIEREVGLLDRDAIKKQTGIDPGGLNVVAYRSDNSLINKGQDEW
                     KKETGLLSIWMLGMFTPSPSVIVAIPFNPGDEKIMGPPVNDDYFGKIAADRLRVEGSH
                     IFFRCDGKSRGKIGLPPLRSKGIMGSYDSANNILTLLICDQPRGKNDYVNSAWQLQED
                     PYSGDALNSYNDGPLEDGSQMGPFYELEASSPGAALKAGEKLTHTQFTIHASGDKNLL
                     NKIALDKLGLSLDAIARAFD"
     gene            complement(4155..5081)
                     /locus_tag="PLZ75_08290"
     CDS             complement(4155..5081)
                     /locus_tag="PLZ75_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOS72429.1"
                     /translation="MKKLIYLFILISFSTAILISCKEGENKKSGEVWKAEHVVFIGLD
                     GWGAYSVEKADMPNVKELMANGCYTLKKRSVLPSSSAVNWASIFMGAGPELHGYTEWG
                     SKVPELPSRELNKNGIFPNIFQLLRDAAPSAEIGVMYEWEGIKYLVDTLSLDYFALAP
                     DYNEHPGVLCEMAEKYILERKPVLTAIIFDNPDHIGHAAGHDTPAYYDDLRELDNYIG
                     RIITAMKESGIYDNSIIIVTSDHGGIGKGHGGKTMAEMETPFIISGKNIRQGGAFNES
                     MMQFDCASTIAHIFNLEQPQVWIGRPMLQVFE"
     gene            5687..6703
                     /gene="rlmN"
                     /locus_tag="PLZ75_08295"
     CDS             5687..6703
                     /gene="rlmN"
                     /locus_tag="PLZ75_08295"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOS72430.1"
                     /translation="MDKKYLCGLNIDEISDLAKAGGFTRSQALIAAVALYRKGESGLQ
                     KTTRIPASMKKYLLESASPGIYPPVKSEKSADGTVKHLFVSPEGKKYESVYIPEAKRR
                     TVCVSTQSGCRIGCLLCVTGKYGFHGNLSAGDIVNQVISNPFRDKVTHVVFMGMGEPM
                     DNTDNVLKAADILTAEWGLAISSRNITVSTVGITSGVKRFLAESFCNLTLSLYSPFPE
                     ERIRVIPAEREYPAREIITIMASWPLRKKRRLSIAYVMIKGVNDSEEHLEELKNILIS
                     TGIRVNILPYHTIGDDSWESSPDDRMHYFRNSLLMSGISASVRKSRGVDISAACGLLA
                     SGMA"
     gene            complement(6732..7250)
                     /locus_tag="PLZ75_08300"
     CDS             complement(6732..7250)
                     /locus_tag="PLZ75_08300"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOS72431.1"
                     /translation="MEYQKIRLRAIEPEDLGLLYEWENNNSYWLISNTIAPFSKYTLK
                     RYISNSHRSIYETRQLRLMIELTEENQTIGTIDIFDFDPFHHRAGIGILIADENQRRK
                     GYATMALKCLCDYCFNTLQLHQLYCNILINNKESIDLFKKAGFTEIGIKKEWIKTSEG
                     YLDEIMFQLIKQ"
     gene            complement(7279..>7616)
                     /locus_tag="PLZ75_08305"
     CDS             complement(7279..>7616)
                     /locus_tag="PLZ75_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72432.1"
                     /translation="PEGRKYNRRVTIGVIDPGTGVVIRHETETPGNLVNPATSKYYIV
                     LEESSRKLGQGYFSNLDITGKLVIRSKEEGAVTRYIIGVFYSREDAAKYLNYVRTKGF
                     GKAYITETE"
BASE COUNT         2002 a         1713 c         1664 g         2237 t
ORIGIN      
        1 acaaaaaatt tatcatcgta caggtttctt gagacaggat aaatttttct cagccattta
       61 taaatctttg aatcttttgg gtcctgtttc ttatcatgtg tcatcagcat tcttaccgcg
      121 gtaaaaatca ggaacccccc aaaaacgtag attatccagg ttagtttgtt gattaaaact
      181 actccgaaga ttatcattat cgctcgaaat acaaccgctc ccaaaacacc gtaaaaaaga
      241 acttcgtgct ggtatttttt tggaattcca aaagaagaga aaatcagagc tataagaaaa
      301 atattatcaa cactcagcga gagttcaaca atatagccgg ttatatattt caataccgca
      361 ttggaggggc tcaggcccgt cggattatca atccatcctt tttcaaaagc aaaataaacg
      421 actccggtaa aaaccagtgc aactgatacc cagacggcag tccatgcagc tgcttccttg
      481 gttttgattt catgaggtga acggttaaaa acacccaggt ccaggaaaag ggccagcaga
      541 ataaatgaga taaaaataat ccagagtgtc atgatttaat ctatttggcc ggtaagtttg
      601 ccattttttc ttttttaatt ataatattaa ttcaactgtt tataaaaact gtactgaacc
      661 ctcagcgtaa atacattatc cgcaatacct ctgtcataac cgctcaggtt ggctgaggtt
      721 tcattctgca accagtcatt gtaaacggtc agtcttatat cacggtttat gtaacaaaga
      781 attcccgtgc caaaggtatt ccaggctata tctcctttcc cggtaccgcc tattcctgtt
      841 tcttttttag ggttttttgc attaggatca taaaagtcaa actttgttac cgccgacaaa
      901 cctgatttga caaaatcctg gatcaggttc aagtaacctc tcctgaaatt tctgatatag
      961 atatctctct cggaaatctc agagtaatcc gggtttgaat tgccttttcc tcccggctgg
     1021 gtgccaaata tatattcccc ggaaaggact gtctttccca tgcagctgct gaattcaatt
     1081 tgaatatcgg ctccggggta ttcacgcttt acatatcctg ccggatcatt ttcatttatt
     1141 gtgaaactat ttcctgccat ctgaaagatt ttggaagcct ccgggaaaac cccgttcaga
     1201 taatatgaat tgcctccatt gatgccagca tggctgccaa gaggcttgta aaccgaaagg
     1261 tggctgaagg ccatggcttt caacttccag gccacaatcc agaagcactc tgatttcggt
     1321 agagttggta ataccgtacc tcagtgtcag attgttaaca aaagttacct tagtcatcag
     1381 tactccctct tttacctgaa acatcttttt cggaactgtc attactccgt cactttggtc
     1441 cggacgaaaa aagccggttt gcatcagaaa gtggatataa aaccggcctg attcctgcag
     1501 ctgcatggca agcagttgat acagcggaga gagagggatt cgaacccccg gtcccgcgca
     1561 agcaggacaa cggttttcga gaccgcccca ttcgaccact ctggcatctc tccttattaa
     1621 atacattttc tgctccggaa tttttatccg gaagcaggca atgtatccga accgcaaatt
     1681 tgaacaaatt atttcaatta tgtcagctta aactgaaaaa gtatggacgg cagcttcttt
     1741 atctgccttt ttcaattatc agcctgctcg tgcaaatcat cttccctgac gaaaatctta
     1801 taaaataaag acctgccgga agactttcaa gtgacagaac catgttgtcc cttcctgaaa
     1861 gatcaataat tctgacagtt tttccggaaa tgtctgctat ttccattctg ttgaagtcca
     1921 tggtgttttc gatccgtatg aaatctgaag cgggattggg gtaaataagc ggtttttcct
     1981 gtacttcccc tgaattgtta aaggaggagg aaacgttacc ctgctctatc ctgatattgt
     2041 ccaggaagaa tctttttgta aggcctgcaa aacggatttt tgccgtcacc gtttctgcag
     2101 gaattgacaa gatactcgtt tgaaaatcgt ctgtcggcgt tattacattg cccagaggca
     2161 aatcattcag gtaaacctgt acagtggatt catccccggg ccagcgggag atgtcgaatt
     2221 tcagaatcag tgcttcttct gacagactga cgggaggagt ttcaatccag ccggttaaag
     2281 aagctgttcc cagttttatt tcaccgccgg cggaatagac cttcgaaccg gtccatcccg
     2341 gaactgcggt tctggaatcg aggtttcccg acgcatcgct tgtcgacggg gtagtgtggg
     2401 tcccggttgt gaatccggaa aaatcttccg caaatacaac ctgctgtcct ttcagaaaaa
     2461 atcccgggca caggagaaag aggatgacag ccgagcctga ggtaagcagc atacttctca
     2521 tcttgataat tatttattcc tttgataact tttagcaatg accaatttac taaaattcct
     2581 gttttggagg aagaattttc atataaaaat cccgctttgt aaaaaaagtt taaccctgtg
     2641 ccgctggctg gctgttcccg gggaaatata tgcgacaggt tcaagttaat atcttgatta
     2701 actgtattca ggcaaattaa tatctcgatt acctgtattc aggttaatat actgactaac
     2761 cgcctgatca cggggatatg tctgaacagg ttaatacagc agcggtgagc cgcgcaaaac
     2821 gggcaaaaat gttatgaaaa ccggtattta tcagtcaaaa gctctggcaa ttgcatcaag
     2881 gctgagaccc agtttgtcaa gggcgatctt gttcaggaga tttttatccc cgcttgcatg
     2941 aattgtgaac tgggtgtgtg tgagtttttc cccggcctta agtgccgcgc ccggcgatga
     3001 ggcttccagt tcgtagaaag gccccatctg tgagccatct tcgaggggtc cgtcattata
     3061 ggagttcagg gcatcaccgg aatagggatc ttcctgcagc tgccacgccg agttcacata
     3121 atcatttttg ccccggggct gatcgcagat aaggagagtg agaatgttgt ttgccgaatc
     3181 atagcttccc ataataccct tcgaccgcag gggaggaagt cctattttcc ccctgctttt
     3241 cccgtcgcac ctgaaaaaga tgtgactgcc ctccaccctg agtctgtcgg ctgcaatttt
     3301 cccgaagtaa tcatcattta caggcggccc catgattttt tcatcaccgg ggttaaaagg
     3361 aattgcgaca ataacggaag gagaaggtgt gaacattcca agcatccaga tggacagcag
     3421 accggtctct ttcttccatt catcctgtcc tttgttgata agcgaattgt ctgatctgta
     3481 agctaccacg ttaagtcctc cgggatcgat acctgtctgt ttttttattg catccctgtc
     3541 aagtaatcca acttcccttt cgatcctgaa cttaagaggt gtgccgctgt agtttacagc
     3601 ttcagcattg caggcaaaaa atgcagatgt atcgttaagc ctgaccactt caaaagggtc
     3661 agtgtcaaga aaagaaggcg tctgccagtt atcatagaca aaatcatcac ctttcctgaa
     3721 gaagattgag aactgtccgc cttcgggtcc gagccataat ctctcttccc cgccaaaagc
     3781 gtttatgtgg ggctgaatga tccctgattc gatgagctga tggtttatcc acccgaagct
     3841 gaagccattt tcaccttcgg cagtgctggt cattaccctg ccctgccatg aaggcaccag
     3901 ggcaacagcc gaattatccc ttttcagttc aataatgtca acatgctttt tcaggaaatc
     3961 gcggttccag gcatattgat ttgctgtttc cgttgtcata tcttttgatt tttgattttc
     4021 agcgcctgat ctgcctccgc aggaataaac aagagcagaa atagtcagaa cgataattat
     4081 ttttttcatt tcccctgttt tttaattttc cttttcatag tgacctgttt gagtcataaa
     4141 ataccggaag aatattattc aaatacctgt agcatcgggc gccctatcca gacctggggc
     4201 tgttcaaggt taaaaatatg tgcaattgtt gatgcacagt cgaactgcat catgctttca
     4261 ttgaaagcac ctccctgcct gatgtttttc cctgagataa taaagggggt ttccatttcc
     4321 gccatggtct ttcctccgtg tcccttgcct attcccccat gatcggatgt aacaatgata
     4381 atgctgttgt catagatgcc gctttccttc attgcggtaa taattctgcc tatgtaattg
     4441 tcaagttccc tcaagtcatc ataataggca ggtgtgtcat gtccggcagc atgtccgata
     4501 tgatcagggt tatcgaagat aatggcagtg agcaccggtt tcctctccag gatatatttc
     4561 tccgccattt cgcacagtac gcccggatgt tcattgtaat caggtgcaag ggcaaaataa
     4621 tccagggaca gggtatcgac caggtatttt atcccttccc actcatacat cactccgatt
     4681 tcagccgagg gggctgcatc acgcagcagc tggaaaatat tcgggaaaat accgttttta
     4741 ttcagttcac gcgaaggaag ctccggaact ttggatcccc attccgtata tccgtgaagc
     4801 tcgggtcctg cccccatgaa tatcgacgcc cagttaaccg cgcttgatga cgggaggaca
     4861 ctccgttttt tcagggtata gcatccgttg gccataagtt cctttacatt cggcatgtcg
     4921 gccttttcaa cgctgtaggc tccccagccg tccagaccga taaatacaac atgttccgcc
     4981 ttccatactt ccccggattt tttgttttct ccttccttgc aggatatgag gatggcagtg
     5041 gaaaatgaga tcaggataaa caggtaaata agtttcttca tgggttggga gttttattca
     5101 gtaagtcatt tcattttcag gagcctgaaa gccacatgtt ttttcaggtt tgatttaatc
     5161 ttccgggttt tcatgcaggt ccaggccgga tatttctcaa taattcagtt ttatccggct
     5221 aaaataataa atgataatga gattctctga ataaacccgc tcattttcaa aattgctttt
     5281 gtatccttcc gggcaaaggg atttggataa ttcggctcaa ggctgaaacc cattggtaaa
     5341 tcggccgggg tcttttcaac tgatatcgga aataatagtt gccgtcgcca gaccccgcct
     5401 ccaaattaaa aacagcggca aaaaaactat cctgcaagaa cgacagatga gccggattaa
     5461 tgttttcatg gttacaatac ctttttggtg ttatgttaat ttttcatttt ctgacgtgaa
     5521 gatttttttt ggtgccccct gccccgggag ctctttatat tataggttaa atttacagcc
     5581 taaacatgcc tgaaacaaaa aaatgtgatg aaccacgtgg cagagtgatg aacgacaatt
     5641 gttgctgggt gagtgaaaag ggattaactt tgagcagctt tctgttatgg acaagaaata
     5701 tctttgcgga ctcaatatag atgagatcag tgatcttgcc aaagccggcg gctttacgcg
     5761 cagccaggct cttattgctg ctgttgcgct ttacaggaaa ggagagtcag ggctgcagaa
     5821 aacgacaaga attcctgcct caatgaagaa atacctcctg gaatcggcat cgccgggaat
     5881 ctatcctccc gttaaatcgg aaaaatcggc tgatggtact gtaaaacacc tgtttgtctc
     5941 ccctgaagga aaaaaatatg aatcagtgta cattccggaa gcaaaacgga ggaccgtatg
     6001 tgtatccact caatcgggat gcaggatagg atgcctttta tgtgtcaccg ggaaatatgg
     6061 cttccatggg aacctgtcag ccggcgatat tgtaaaccag gttatttcca atccgtttcg
     6121 tgataaggtt acgcatgtgg tttttatggg gatgggtgag ccgatggaca ataccgataa
     6181 tgttcttaag gctgcagaca tactgactgc tgaatgggga ctggcaataa gtagcaggaa
     6241 tatcactgtt tcgacggttg ggataacatc tggggtgaag agatttttgg cggaaagctt
     6301 ttgcaacctg actctgagcc tctattcccc ttttccggag gaaagaataa gggttattcc
     6361 tgcggaaaga gaatatccgg cgagggaaat catcacgata atggcttcct ggccgctcag
     6421 gaagaaaaga cggctgagca ttgcctatgt gatgataaaa ggggtcaacg acagtgagga
     6481 gcatcttgaa gagctgaaaa atattctgat cagtacgggc ataagggtaa atatcctgcc
     6541 gtatcatacc attggtgacg attcatggga atcttcacct gatgaccgaa tgcactattt
     6601 caggaacagc ctcctgatgt caggcatatc ggcttcagtc cggaagtcaa gaggcgtgga
     6661 tatttcagct gcatgcggat tgctggcctc cggaatggca tgatgcggga tcagccgggc
     6721 ggggacgggt attattgttt tatcagctgg aacattattt catccagata tccttcggaa
     6781 gttttaatcc attccttttt tattcctatt tccgtgaatc ctgccttttt gaaaagatct
     6841 atactttctt tgttgttaat cagaatattg cagtaaagct ggtgaagctg taatgtattg
     6901 aaacaataat cacaaaggca tttgagtgcc atggtggcgt atcctttcct tctctggttc
     6961 tcatccgcta taaggatccc gataccagcc ctatggtgga aaggatcaaa gtcgaatatg
     7021 tctattgtcc ctattgtctg gttttcttcc gtcagctcaa tcatgagcct cagttgcctt
     7081 gtttcataga tactccggtg ggaattgctg atatatcttt tcagtgtata ttttgaaaag
     7141 ggggcaattg tattgctgat cagccagtag gaattattat tctcccattc atagagcaat
     7201 ccgagatctt ccggttcaat tgcccgcaat ctgattttct gatattccat acaaaagtcc
     7261 tggttctgct gtttggactt attcggtttc ggtgatatag gctttaccga atccctttgt
     7321 ccggacataa ttcagatact tggcggcatc ctccctgctg tagaagactc cgatgatata
     7381 cctggtcacg gcaccttctt cctttgacct gataaccagt ttgccggtga tatcaagatt
     7441 gctgaagtat ccctggccga gtttccggga tgattcttca agcacgatat agtatttgct
     7501 tgttgcagga ttgaccagat tcccgggtgt ttcggtctca tgcctgatta ccactcccgt
     7561 acccggatca attacgccta ttgtcactct tctgttatat ttccggcctt cagggt
//
LOCUS       DAOSPX010000148         7576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9093_length_7576_cov_3.145326, whole genome shotgun sequence.
ACCESSION   DAOSPX010000148 DAOSPX010000000
VERSION     DAOSPX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9093_length_7576_cov_3.145326"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLZ75_08310"
     CDS             <1..283
                     /locus_tag="PLZ75_08310"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOS72433.1"
                     /translation="IAGKGAHVEPFTIKNHTEIVLKELEDELIKAGSSMEKVLKVNVY
                     LDDIKDYQGMNEVYAGRFGSKPPVRTTVAVAKGGVPGNSLVEMDCIAYI"
     gene            299..1516
                     /locus_tag="PLZ75_08315"
     CDS             299..1516
                     /locus_tag="PLZ75_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS72434.1"
                     /translation="MKRRDVIKGLTILPVAGVAMGSTIPLVSSCASPVKRDVLRELGL
                     RTFINAAGTYTFMTGSLLPDEVMEAMVAASKEFVLLDDVQDKAGEKIAEICHAEAATV
                     TAGCWSAMVLGTAGVITGKDQAKIGQLPDLEGLKSEVITQVSHNTGYVHALKNTGVKI
                     IAVETVEELEKAINEKTAMLWFLNYTAPLGKIQDREWVELGRKHNIPTMIDIAADVPP
                     VENLWKFNDMGFDLVCISGGKGLRGPQSAGILMGRKDLIEAARLNAPPRGGNIGRGMK
                     VNKEEVVGMYAALERYVNLDHDREWKIWEDRIAHIENAAKKTAGVRTSVTVPPIANHT
                     PTLNIEWDPEVIKVSREEFQERLRRGNPSIEVVAGGGNSINITAFMMEPGQEIIVAAR
                     IAEELTKASATTS"
     gene            complement(1594..1863)
                     /locus_tag="PLZ75_08320"
     CDS             complement(1594..1863)
                     /locus_tag="PLZ75_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72435.1"
                     /translation="MAYDRQPSIRALGTDSRDGIVMEVNVPEESDVYVDVKNRFGDVV
                     WRLKADGLEPGKHEVVWDGFSQPGMYTMYVKGMGWDAEREMVIYT"
     gene            complement(1936..3198)
                     /locus_tag="PLZ75_08325"
     CDS             complement(1936..3198)
                     /locus_tag="PLZ75_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS72436.1"
                     /translation="MKRRDFIKLSSAAGVMGLINPSHDALASSGLQGGNGFDLHPFIK
                     EHPEAVFINLTSVGAPDDSQAIHDAAFKLAGEMFVRTSDGSGYPLSTAITCKPNWTCT
                     GRPDPNDPISALGITTDLNYIEGFLKGVKTLGPEKFYLRECACTHQWEPLGYYDMARR
                     NNFDLKDLTSKDYWDLPEGEIIFREVNGGVVFREVGIMAPMTADGTFLINLAKLKAHQ
                     MGITAAVKNLQGITARRFHQFCGGNYNIFKTYDKRYHKFFQPDYMEKIAELQKIHEDR
                     GMPRWKSKVDRPPYGGGFWMEQWVQRMIDAYSVIPTGINIVEGVYAQDGDGFNGGPHD
                     GKPGKFMSNNVIFGKDAFRVDIISHWLAGHEPGNFGLFHIGIERGVSDVLDPFDIPVY
                     LWENGKATKVNLDSLKRTPLVTLYLRKN"
     gene            complement(3291..4904)
                     /locus_tag="PLZ75_08330"
     CDS             complement(3291..4904)
                     /locus_tag="PLZ75_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS72437.1"
                     /translation="MKRRDIIKSLTILPFAGSFLPLESALSKGTGPLVPGPNIYQSIG
                     VEPVINCMGTFTIISGSLERPEVIETIGYAARHFVQMDELAMGIGRRLAELTGAEWGM
                     VSAGCAAGMKHVTAACVSGGNPEKLIRIPDLTGLDKTEVVIPRSSRNAYDHAIRNIGI
                     SIITVDSLEELEKSLNSRTAMIYVMTGNDTLPGNRFAIDKIAALAKPKDIPFLVDAAA
                     EDLTIPNVHLLAGASIVAYSGGKAMCGPQGAGLLLGRKDLLMSAWQASSPHHGTGRDN
                     KVGKEEMMGMLAAVEAWIKRDHDEKYRTWMTYLDNISKRVSKIKGIKTEIREPQGLDL
                     NNRSPVLMISWDSSGFNITGPDIAEILANTKPRIALRGTGNDEDGTSSLRVNSGQMQP
                     GDDKIVADRIFELLSKKYPKPSAMAAPAADISGRWAVDVQFFSSKGSHAFLLEQDGNW
                     LQGSHKTEFSFREIVGTIEGDQVKLSSTERLTADSVPYIFSGTLSGDTISGQIHLGEY
                     LNATFTARKNDYRVSRRQIRYPKGQPLAT"
     gene            complement(4947..6269)
                     /locus_tag="PLZ75_08335"
     CDS             complement(4947..6269)
                     /locus_tag="PLZ75_08335"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HOS72438.1"
                     /translation="MEREVAIISGAGAMFCAGHMRQSVLNVLRPLYLLLLFSVLRPGA
                     MIAQDIDILLRGGHLIDAKNKINGRMDIAVSKGRIVKVSPSIPSEKAERVIDLNGLYV
                     TPGLIDIHTHVFVGPEAGFANGFYSVSPDDFTFKSGVTTVADAGTSGWRSFPLFKKQV
                     IDRSQTRVLAFLNIAGSGMTGFPSEEDINDMDARMTSLVIGQYPDIIVGVKIGHYRGS
                     DWTPFDRAAEAAGISDVPLLVECHLPLLPLEGILSRMKEGDIYTHSFCVASDRECLLD
                     EKGRIKPFVAEARKKGIRFDVGHGGGMFHFNVAIPAFRQGLWPDSFGTDLHRISMNSG
                     MKNMLDIMSKYLNIGMSFEDIISRATWNPAQSLKREDLGHLGEGAVADIAVFSIREGR
                     FGFIDAAGYRLDGDRRIETELTIRAGRVVYDQNGISARSWLENQTIKF"
     gene            complement(6254..>7576)
                     /locus_tag="PLZ75_08340"
     CDS             complement(6254..>7576)
                     /locus_tag="PLZ75_08340"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HOS72439.1"
                     /translation="MESGVYSINIMMMNRIKKITMRTGLAKACLLLFLFILAGIGLSH
                     GQTIDILLKGGHVIDPANGIDKKMDVAIAGGKILRVAPAIPVRDANKVIDVSGMYVTP
                     GLIDMHVHAFHGTDQGSYIADGWDALPPDGFTFRAGVTTVVDAGSAGWRNFRKFKEQT
                     IDRSRTRVLAFLNIVGTGMYGRFEEQDVSDMNPVMVSYMIKRLFPDILVGVKSAHYWG
                     PDFTQVDHAVEAGRLADVPVMVDLGEHDPPLSIEELFMNHLRPGDIWTHTYANAPWDR
                     EVPVDENGRVKPFVFEAQKRGIIFDVGHGGGAFHWKQAIPSVQQGFIADVISSDLHIG
                     SMNSGMKDMANLLSKFLNMGLSLEDVIRRATLNPAKVIKRPELGTLSPGAEADVAVFR
                     LHTGDYGFLDVRGTKMKGNKKLEAELTIRAGRIVWDLNGIGAPVWNEK"
BASE COUNT         1951 a         1966 c         1689 g         1970 t
ORIGIN      
        1 catagccggt aaaggcgcac atgtcgagcc ttttacaatc aaaaaccaca ctgaaattgt
       61 gctgaaggag cttgaagatg agctcatcaa agccggatcc tcaatggaaa aagtccttaa
      121 agtaaatgta tacctagatg atataaagga ctaccaggga atgaatgagg tgtatgcagg
      181 acgcttcggc agcaaacccc cggtccgtac aaccgtggca gtggccaagg gtggtgtgcc
      241 cggaaactca ctcgttgaaa tggattgcat cgcttatatt taataagaat aagctgagat
      301 gaaacgcaga gatgttatca aaggattaac aattctgccg gtggccggag tggcaatggg
      361 aagtaccatt ccattggtat cttcctgtgc ctcaccggtt aaacgggatg tgctgcgcga
      421 actcggactc aggactttca tcaatgcagc cggcacctat acattcatga cgggttccct
      481 gctgccggat gaagtaatgg aggcaatggt tgctgcatca aaggaatttg ttcttctgga
      541 tgacgttcag gacaaggccg gtgaaaagat tgcggaaatc tgtcatgccg aagcagccac
      601 tgtaacagcc ggctgctggt cggcaatggt actcgggact gcaggggtaa ttaccggaaa
      661 ggatcaggca aaaatcggac agctccccga tcttgaagga ctgaaatctg aggtaatcac
      721 tcaggtaagt cataataccg gttatgtaca tgcactcaag aacacaggag taaaaattat
      781 agccgtggaa acggttgagg aactggaaaa ggctatcaac gaaaagactg ccatgctctg
      841 gtttcttaat tataccgccc ctctcgggaa aattcaggac agggaatggg tggaactggg
      901 aagaaagcat aatattccca caatgattga tattgctgct gatgttccgc ccgttgaaaa
      961 tctctggaaa tttaacgaca tgggatttga cctggtctgc atctccggtg gaaaagggct
     1021 cagaggaccg caaagcgccg gtatcctgat gggaaggaaa gatcttatcg aggcagcccg
     1081 gcttaatgcc ccgccccgcg gaggcaacat aggcagaggc atgaaggtaa acaaggaaga
     1141 agttgtcgga atgtatgccg cgcttgaaag atatgtcaat cttgatcacg acagggaatg
     1201 gaaaatatgg gaagacagga tcgcacacat cgaaaacgcg gcaaaaaaga cagccggagt
     1261 aaggacaagc gttaccgttc ctccgatagc caaccacact cccacactga atatcgaatg
     1321 ggatcctgaa gtgataaagg tcagccgcga agaattccag gaaagactcc gcaggggaaa
     1381 tccatccatt gaggtggttg ccggcggtgg aaacagtatt aacataacgg ctttcatgat
     1441 ggaaccaggg caggaaatta ttgtggcggc aaggattgcc gaagaactga caaaagcttc
     1501 tgccaccact tcttaaatgg gttctgacag aagctttttt taattccctg ctccttttag
     1561 ttacctgata cccgggaagg gacttcccgg gtattaggtg tatataacca tttcacgttc
     1621 ggcatcccag cccattccct taacatacat tgtgtacatg ccgggctgtg aaaatccgtc
     1681 ccaaaccact tcatgctttc cgggctcaag gccgtcggcc ttcaaacgcc agacaacatc
     1741 cccgaaacgg tttttaacgt ctacataaac atcactttct tccgggacgt ttacttccat
     1801 aacaatacca tccctgctgt ccgttccgag tgcccttatt gaaggctgcc tgtcataagc
     1861 cattgtaccc gttttccatg cggaatagtc aaacggctca tttaccaggt gatagcactc
     1921 ctcatcatgt ccgtctcagt ttttcctcag ataaagagta accagaggag tgcgcttcag
     1981 gctgtccaga ttcacttttg ttgcctttcc gttctcccac agatatacag gtatgtcaaa
     2041 aggatcaagg acatccgata caccgcgttc aatcccgata tggaacagtc cgaaattgcc
     2101 tggttcatga cctgcaagcc agtgagaaat aatatctacc ctgaatgcat cctttccgaa
     2161 aatcacgttg ttggacatga attttccagg cttcccgtca tgcgggccgc cattaaagcc
     2221 atcaccgtcc tgagcataca caccttccac aatatttatc ccggttggta tgacactgta
     2281 ggcgtctatc attctctgaa cccattgttc catccagaaa ccgcctccgt aagggggcct
     2341 gtccaccttg cttttccatc tgggcattcc acggtcttca tgaattttct gaagttccgc
     2401 aattttctcc atataatcgg gctggaagaa tttgtgataa cgcttgtcgt aggtcttgaa
     2461 aatattgtaa ttcccaccgc agaactggtg aaatcttctt gctgttattc cctggaggtt
     2521 tttcactgca gctgttattc ccatctgatg ggctttaagc ttggccaggt tgataaggaa
     2581 ggtaccgtcc gccgtcatgg gtgccatgat accgacttcc ctgaacacca ctcccccgtt
     2641 cacttccctg aaaatgattt ccccttcggg cagatcccag taatccttgg aagtcagatc
     2701 tttcaggtcg aaattatttc tccttgccat gtcataataa cccagcggct cccactgatg
     2761 tgtgcaggca cattccctca ggtaaaactt ttcaggtccc agtgttttca cacctttaag
     2821 aaatccttca atatagttga gatccgtggt tattcccagg gcagaaatcg ggtcattcgg
     2881 atccggcctt cccgtacatg tccagttggg cttgcaggtt attgccgtgg ataagggata
     2941 tccgctgccg tcactggttc tgacaaacat ttcccctgcc agcttgaatg cagcatcatg
     3001 aatggcctgg ctgtcatccg gtgctccaac cgaagtcaga ttaatgaaaa cagcttccgg
     3061 atgctccttt atgaagggat gaaggtcaaa accatttccg ccctgcaggc cggaagaggc
     3121 caaagcatca tgcgaaggat taataagtcc catcactcct gcagcagatg agagttttat
     3181 aaagtcacgt cgtttcattt atttactatt agattcagtt taagtaatta ctatttatca
     3241 taaaacacat tatttcaata tttgtttgga taagcccgca ttcctccggc tcaggttgcc
     3301 agcggctggc ctttaggata tcttatctgc ctgcgggaaa cccggtaatc attcttcctg
     3361 gcagtaaatg ttgcattcag gtattctcca agatgaatct gtccgctaat ggtatctccc
     3421 gataaagttc cggagaagat atagggcaca ctgtcggcag tgagcctctc ggtactgctc
     3481 agtttaacct ggtcaccttc aatggtaccg acaatttccc tgaacgagaa ttcagtcttg
     3541 tgagatccct gcagccagtt tccgtcctgt tccagtaaaa atgcatgact ccctttgctg
     3601 ctgaagaact gaacatcaac tgcccatctt ccgcttatat cagcagccgg cgcggccatc
     3661 gccgaaggtt tcggatattt cttcgacagg agttcaaaga tcctgtctgc aacaatctta
     3721 tcatcccccg gctgcatctg ccccgaatta actctcaggg atgaagtccc gtcctcgtca
     3781 ttgccggtac cgcggagtgc tattctcggc ttggtgtttg cgaggatctc ggcaatatcg
     3841 ggacctgtaa tattaaatcc tgaggaatcc catgaaatca tcaggaccgg gcttctgtta
     3901 ttcagatcaa gaccctgggg ttcccttatt tccgttttaa tgccttttat cttagatacc
     3961 cgctttgaaa tattgtcaag ataggtcatc catgtccggt atttttcatc atgatcacgt
     4021 ttgatccatg cttccactgc tgcaagcatt cccatcattt cctccttgcc gaccttattg
     4081 tccctgcctg tcccgtgatg gggggaactt gcctgccagg ctgacataag caggtctttc
     4141 cggcccagga gcaaaccggc accctgaggg ccacacatgg ccttccctcc gctgtaggca
     4201 acaatggagg cccctgccag gagatgaacg ttcggaatgg tcagatcctc tgccgctgca
     4261 tcaacaagga agggaatatc ttttggtttt gccagggcag ctatcttgtc gatggcaaac
     4321 ctgttccccg gcagggtgtc atttccggtc ataacatata tcattgcagt acgggaattc
     4381 agcgattttt ccagttcctc aagactgtca acagtaataa tgcttattcc tatatttcgt
     4441 atggcatgat cataggcatt cctggaacta cgggggataa caacctctgt tttgtcaagt
     4501 cctgtcagat cgggtatcct gatcagtttt tcaggattgc cgcccgaaac gcaggcagct
     4561 gtaacatgct tcattccggc ggcacatccg gcagatacca taccccattc agccccggtc
     4621 agttcagcca ggcgtctgcc tattcccatt gccagctcgt ccatctggac gaaatgacgg
     4681 gcagcatatc ctattgtttc aatgacctcc ggccgttcaa gggatccgct gataatggtg
     4741 aatgtaccca tgcagtttat aaccggctcc acgcctatcg actggtaaat attcggaccc
     4801 ggtacaagag gtcccgtgcc tttggataaa gctgattcca ggggaagaaa gcttcctgca
     4861 aaaggcagaa ttgtcagact tttaataata tctcttcgtt tcatggtgtt ttgttcagtt
     4921 aatattcagg atttctgtaa tttcagttaa aatttgattg tctggttttc aagccagctt
     4981 ctggctgaga ttccgttctg gtcatatacc accctgcccg cgcgaatggt aagttcggtc
     5041 tctattctgc ggtctccgtc aagcctgtat cccgctgcat caatgaaacc gaatcttcct
     5101 tccctgatgc tgaaaacggc aatatcggcc acggcccctt cgccaaggtg tccgagatcc
     5161 tcccgtttga gagactgtgc cggattccag gtagccctgg aaatgatgtc ttcaaaactc
     5221 ataccaatat tcaggtactt cgacataata tcaagcatgt ttttcatccc tgaattcatg
     5281 cttatgcggt gaagatcggt gccgaaggaa tcgggccaca gtccctgcct gaatgcaggt
     5341 atggcaacat tgaaatgaaa catacctccc ccgtgcccca cgtcgaacct tattcccttt
     5401 ttccgtgcct cagccacaaa gggctttatc cttccctttt catccagaag gcattccctg
     5461 tcggaagcaa cacaaaagga atgagtgtaa atatcccctt ccttcatcct cgaaagtatt
     5521 ccttccagcg gaagcagtgg cagatgacat tcaaccagaa gaggaacatc agaaattccg
     5581 gccgcttcgg cagccctgtc aaagggagtc cagtcactcc ccctgtaatg accgatcttt
     5641 actccaacaa ttatatcggg atactgacct attaccagcg aagtcatacg ggcatccata
     5701 tcatttatat cctcttccga gggaaatccc gtcatacccg atcctgcaat attcagaaaa
     5761 gcaagcactc tggtctggga ccggtcaatc acctgcttct tgaacagggg aaaacttctc
     5821 cagccggaag ttccggcatc tgcaacggta gtgacacctg atttgaaggt aaaatcatca
     5881 ggagaaacac tgtaaaaacc atttgcaaat cctgcttccg gaccaacaaa aacatgagtg
     5941 tgaatatcaa ttagtccggg ggtaacatat aatccgttca gatcaattac cctttctgcc
     6001 ttttctgaag gaatcgacgg agatactttt acaatcctgc ctttggaaac ggcaatatcc
     6061 atccgtccgt ttattttgtt tttagcatca atcagatggc cgccccgcag cagaatgtca
     6121 atatcctgtg caatcatagc gcccggacgg agcactgaga acagcagaag caaatacagc
     6181 ggcctcagaa cattcaggac tgactgccgc atatgtccgg cgcaaaacat ggcgcccgct
     6241 ccggatatta ttgctacttc tcgttccaca ccggcgctcc aatgccattg aggtcccaca
     6301 caatccttcc cgcacgaatt gtgagttcgg cctcaagctt cttgtttcct ttcatttttg
     6361 ttccacgcac atccaggaat ccgtagtcac ccgtatgaag cctgaacaca gcaacatcgg
     6421 cttcagcacc gggactgaga gtgccaagtt ccggtctttt aataactttt gcaggattaa
     6481 gtgttgcgcg tctgataaca tcttccagcg aaagccccat attgagaaat ttcgaaagca
     6541 ggttcgccat atccttcatg ccgctgttca tgcttccgat atgaagatca gaactgatca
     6601 catcagcaat gaatccctgc tgtaccgaag gtattgcctg tttccagtga aaggctccgc
     6661 ccccgtggcc aacatcaaat ataattcctc tcttctgggc ttcgaaaaca aacggcttta
     6721 cccttccgtt ttcatccacc ggcacctcac ggtcccaggg agcattggca taggtatgag
     6781 tccagatatc cccggggcgc aggtgattca tgaacaattc ctcaattgaa agaggcggat
     6841 catgttcccc cagatcgacc attaccggaa catcagccag cctcccggct tcaactgcat
     6901 gatcaacctg cgtaaaatcc ggtccccagt aatgggccga cttgacacca acaaggatat
     6961 ccggaaacag ccttttaatc atatatgaaa ccattacggg attcatgtca cttacatcct
     7021 gttcttcaaa tctgccgtac attcctgtgc cgacaatatt gagaaaagca aggactcttg
     7081 tccgtgatct gtcaatggtc tgttccttga atttcctgaa atttctccat ccggcagacc
     7141 ctgcatccac aaccgtggta acacctgccc tgaaagtgaa tccgtccgga ggcagggcat
     7201 cccagccatc agctatatag gatccctgat ccgttccgtg aaaagcatga acatgcatgt
     7261 cgatcaggcc gggggtaaca tacatgccgc tgacatcaat aaccttgttt gcgtccctga
     7321 caggtatggc gggagccacg cggagaattt tgcctcccgc aatggccaca tccatttttt
     7381 tatcaatgcc gttggcagga tctattacat gtccgccctt aagcagaata tcaattgtct
     7441 gcccgtggga caaaccaatt ccggcaagaa taaataaaaa taacagcaga caggcttttg
     7501 ccaggcctgt ccgcatggtg atttttttaa tcctgttcat catcattatg ttgatactgt
     7561 aaacaccaga ttccat
//
LOCUS       DAOSPX010000149         7552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9128_length_7552_cov_4.369214, whole genome shotgun sequence.
ACCESSION   DAOSPX010000149 DAOSPX010000000
VERSION     DAOSPX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9128_length_7552_cov_4.369214"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLZ75_08345"
     CDS             complement(<1..217)
                     /locus_tag="PLZ75_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72440.1"
                     /translation="MKAAGILIAVFLFLSPAFYLPAQLLPYQNPQLGTAERVDDLVQR
                     MTIEEKVGQMLKLSLENIIRDENGRVPE"
     gene            complement(214..1413)
                     /locus_tag="PLZ75_08350"
     CDS             complement(214..1413)
                     /locus_tag="PLZ75_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72441.1"
                     /translation="MKKNAFIPKLTFMLAVCFLSLNTIAGDLKTGSAAVKITPPPGAP
                     LAGYYYDRAADGVHDDLFAKAIVFEKDGVKAVVISCDLISVPAEIVSAARKLIEKNTG
                     ISPGYVMISATHSHTGPVMPKKIDRYNNLRNAAYVKYLSQLPGLIAESAILANSALAD
                     AKLSVGKGHEESISFNRRFFMTDGTVGWNPGKLNPRIIEPAGPIDPEVYVLYAETIEE
                     KPLATLVNFALHLDNVGGTEISADMPFTLSSILGRVKGSEMVTLFAQGCAGNINHINV
                     RTKDPQKGHGEAQRIATVLSGEVLKTYTRMKVLDIDNISASQEIVRLKLPDVSPDELP
                     GALEVISRTGKPDAPAFLELVNAHKVVDVLDRNGEPVNSRVAPGSGEILAERALALLN
                     GLKSAGQ"
     gene            complement(1434..3575)
                     /locus_tag="PLZ75_08355"
     CDS             complement(1434..3575)
                     /locus_tag="PLZ75_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72442.1"
                     /translation="MKRRILYDHNGKSKFLLLLQKGKSFSAVFILLLLSSSITLNESG
                     SAADMTAQEISPGKTIPYSLPWDDMPVDLSFIFAGEKPAGKHGFLQVRGNEFVFEDGT
                     PARFWGTNFNSGQCFPPHEHSARIAKRLAKTGVNIVRFHQMDAEWASPNIFQCSPGEN
                     KTNTMNLDPDSMDRLDYLIYNLKKEGIYIYMDLLTYRRFKTGDGVEAAEQLGDAAKPY
                     STFNRKLIELQKKFNYDLWTHVNPYTKLAYKDDPAIVLVEVTNENDLFSQKVSLEPYR
                     SELEKMYRSWAKKGKIRLPEGNVDFTVTDNTMLQFFTDVSRDYYREMISHMRETGVKI
                     PVAGTNWTRNAAHLSAQTIGDFTDSHAYWYTFRIWGMNNKRFMNDPMTGSTGNMLTGL
                     SFYNVAGKPFFVSEWDNPWPNEWRAESPLLMAAVGAMQGWGGFAIHTYRYGNDEDVDM
                     IGKPITSDAIGGVYYRGGVFDTFNDPAKFGLFYHAALIMRRGDVQTPEKISYIKLKDI
                     SATSAIKALPLAAEKQRIKIVLPGDESISAGLADSDQPFADTGKGEVISDTKELYRNI
                     TKKFGWIDTPRTKAVYGFTGGEGPVKLTDLTISVKNDFATVAVSSLTDDPISASPNML
                     LTTVGRADNTGSRYNEDHTVQLDPGHGPVQVNVIEAVIEIRTGRSNLRVMSVNPRGFI
                     TGYIPSEYKDGVFRFEVGKEYQSMYYLIQSL"
     gene            complement(3641..5227)
                     /locus_tag="PLZ75_08360"
     CDS             complement(3641..5227)
                     /locus_tag="PLZ75_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72443.1"
                     /translation="MKHICNVLERSLLMSVIAVFLISADLHEHSGNFKIGWASADITP
                     DKPVLIAGQFHARVMEGVMDPITATALALESGSGPSSAKIIMISCDLVSISDGMRDGS
                     PDNLRDNVRELLKHTIPEVSPEQIFLNATHTHTAPLVGSARNSESVYGVELDVLSPAD
                     CQKYIAERIARAAEKAWKSRRAGGISFGLGHAVVGHNRLTVDKDGRSTMYKRPDTPGF
                     SHVEGYEDHSLNLLYTWDDRNNLTGIVVNVPCPAQVTEGRFMISADYWNETRIELRGR
                     LGDEIHILPQCSAAGDQSPHVLIGEKAEARMQKLMFSDSITGGQTLAHRKQIALRIAD
                     AVTSILPYMKSSIDRDPVFEHKMEIVELSRRLIDTADVNNALRESETYREQYNELISE
                     IRNNPDATLKPRWYTGISQIHTLMKRGESVKERYELEKIQPRMPVEVHVLRIGDMVIA
                     TNPFELYLDYGMRIKGRSPAIQTFLVQLAGSGSYLPPQRSVAGGSYGAVPASTLMGPE
                     GGQELVEKTLEMISSLWQVQ"
     gene            complement(5342..6232)
                     /locus_tag="PLZ75_08365"
     CDS             complement(5342..6232)
                     /locus_tag="PLZ75_08365"
                     /inference="COORDINATES: protein motif:HMM:NF040125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6528 family protein"
                     /protein_id="HOS72444.1"
                     /translation="MKRIAFLLPFLTVLLFPACSEAADEIIACGDDQVIIIDRNKSAD
                     TNVFITWKWKIGEIDDLPGDYLKYMRTTDECKPVDNNKKILIASSSGGVVLTDRKTKK
                     SLFYAYVPMAHSAELLPGDRIVVALSTNPEGNCIQLFDIGKPDKVIYQDSLYSGHGVV
                     WMPRYQRLFALGFDELRSYSLKDWDTDNPSLQPDRKWQLPDNGGHDLNNITDDRLIIT
                     THNGVWTFDIPDEQFREFELLKDVRNVKSVNYNESTGELLYTKGEISWWTHNIYSRNP
                     DKTITVPDIDLYKTRVIRRR"
     gene            6447..6866
                     /locus_tag="PLZ75_08370"
     CDS             6447..6866
                     /locus_tag="PLZ75_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72445.1"
                     /translation="MRKALAILITGLMFLTGMRISIDHHYCGGELAARRLSLTGTAAS
                     CGMEDQKHSFSFQTEFGSICCEDQFASYGINNIFIPEYFRLTHPDGGKKVRFFQAPPP
                     HLSPAVSDQTETAFRNKPPGEKPESEVSLSRICVLRI"
     gene            6973..7356
                     /locus_tag="PLZ75_08375"
     CDS             6973..7356
                     /locus_tag="PLZ75_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOS72446.1"
                     /translation="MKSFNFAFAVLLMAGLSMSAAAQTGQPDRTDRSVPGTESVRTES
                     FRVWGKCGMCKTRIETTVKSEGASAASWDQKTQLLTVSYDPAKTNSDALKKKLAAAGH
                     DTEKYKAPDDVYSKLPACCHYERSK"
BASE COUNT         1808 a         1863 c         1737 g         2144 t
ORIGIN      
        1 cttcgggaac acgtccgttt tcatcgcgta ttatgttttc caggctcagt ttcagcatct
       61 ggccaacctt ttcctctatc gtcattctct gaacaagatc atccacgcgt tccgcagtac
      121 caagctgagg attctgatag gggagcagct gcgcaggcag gtaaaaggcc gggctcagga
      181 aaagaaaaac cgcaatcaat atcccggctg ctttcattgt ccggcagatt tcagaccgtt
      241 cagcagtgca agggcccgtt cagccagtat ttcgcccgac ccgggagcaa cacgtgagtt
      301 caccggttcc ccgttcctat ccagaacgtc aaccacctta tgggcattca ccagttccag
      361 gaatgccggt gcatcaggtt ttcctgtccg tgagatgact tccagggctc cggggagttc
      421 atcaggactg acatccggca gcttcagcct gacaatttcc tgacttgcgg atatattgtc
      481 aatatccaga actttcatcc tggtatatgt tttcagtacc tctcccgaaa gaacggttgc
      541 tatgcgctga gcttcaccat gacctttctg ggggtccttc gtcctgacat tgatgtgatt
      601 gatattgccg gcgcagccct gggcgaagag ggtaaccatt tcactcccct ttaccctgcc
      661 caggatggaa gacagagtga agggcatatc tgcagaaatt tctgtccctc ctacattatc
      721 gaggtgcagt gcaaaattta ccagggttgc aagaggcttt tcttcaatag tttcagcata
      781 caggacatag acttccggat ctatgggacc ggccggttca atgatccggg gattcagttt
      841 cccgggattc catcccacgg taccatctgt catgaaaaac cttcggttga agctgattga
      901 ttcctcatga cctttcccca ctgatagttt tgcatcagca agtgcagaat tggccagtat
      961 ggcactttcc gctatcagtc cgggaagttg cgaaagatat ttcacatatg ccgcgttccg
     1021 gagattattg tacctgtcaa ttttcttagg cataacaggt ccggtgtgag aatgagttgc
     1081 actgatcatc acgtagccgg ggcttattcc cgtattcttt tcaatcagct ttcttgctgc
     1141 tgagacaatc tccgccggta cggatatcag gtcgcacgaa ataacaactg ctttgactcc
     1201 gtctttttca aaaacaatgg ctttggcgaa aaggtcatcg tgaacaccat ctgccgcccg
     1261 gtcataataa tagcctgcca gtggtgctcc cgggggcgga gtgattttca ccgcagctga
     1321 acctgttttc agatcaccgg caatagtgtt cagtgaaagg aaacatacag ccagcatgaa
     1381 tgtaagtttt ggaatgaaag cgttcttttt catcactgat catttttgct tatttacaaa
     1441 ctttgaatga ggtaatacat tgactggtat tcctttccca cttcgaaacg gaagactccg
     1501 tctttatact ccgaaggtat atagccggtt ataaagcccc ggggattaac agacatgacc
     1561 cgtagattgc tcctgccggt cctgatctca atcacagctt caatgacgtt gacctgtaca
     1621 ggtccgtgtc ccgggtccag ctgaacagta tgatcttcat tatatctgct cccggtattg
     1681 tcagcccgtc ctactgtagt cagcagcata ttcggggaag cggatatcgg atcatcggtc
     1741 aggctgctga cagcaacagt tgcaaaatcg ttcttcacac tgattgtcag atccgtcagt
     1801 tttaccggac cttccccacc ggtgaatccg tatactgctt ttgtccgggg agtatcaatc
     1861 cagccaaatt ttttggtgat atttctgtaa agttccttgg tgtcagatat tacttctccc
     1921 ttgccggtat cagcaaaagg ctgatctgaa tcagccagtc cggcggatat gctttcgtcc
     1981 ccgggcagaa ctatttttat tcgttgtttt tctgctgcaa ggggcagggc tttaatggcg
     2041 gaagtggctg agatatcctt cagtttaata tagctgatct tttccggcgt ttgaacatct
     2101 cctctgcgca tgataagtgc cgcatggtag aaaagtccga atttcgccgg gtcattgaat
     2161 gtatcaaata ccccgcccct gtaataaact ccgcctattg catcagaagt gattggtttt
     2221 ccgatcatat ccacatcttc atcattacca tagcggtagg tatgaatggc aaatccaccc
     2281 catccctgca ttgctccgac agcggccatc agcagggggc tttctgccct ccattcattt
     2341 ggccatggat tatcccattc cgacacgaaa aacggcttgc ccgcaacatt ataaaaggag
     2401 agtcccgtga gcatgttgcc ggtactgcca gtcatcggat cgttcatgaa ccttttatta
     2461 ttcattcccc atattcggaa ggtgtaccag taggcatggc tgtcggtgaa atcgcctatg
     2521 gtctgtgccg acagatgggc agcgttcctg gtccagtttg ttcctgctac aggaatcttt
     2581 acacctgttt ccctcatgtg ggaaatcatt tcccgatagt aatccctgct tacatctgtg
     2641 aaaaactgca gcatggtatt gtctgtcacg gtaaaatcca cgttcccttc aggcaatctg
     2701 atttttccct ttttagccca gctccggtac attttttcca actcgcttct atagggttca
     2761 aggctaacct tctgactgaa gaggtcattt tcatttgtca cctcaaccag tactattgcc
     2821 ggatcatcct tatatgccag ttttgtgtaa ggattaacat gcgtccacag atcatagttg
     2881 aattttttct gtaattctat cagcttcctg ttgaatgtgc tgtagggttt tgcagcatca
     2941 ccaagctgtt ccgctgcctc aacaccatct cccgttttga atctcctgta tgtgagcagg
     3001 tccatgtaaa tatatatgcc ttctttcttc aggttgtata tgaggtaatc cagcctgtcc
     3061 atgctatcag gatcgagatt cattgtattt gttttgttct cacccgggga gcattgaaaa
     3121 atattcggag aagcccattc agcatccatc tggtgaaacc ttacgatgtt cactccggtt
     3181 ttagcaagcc gttttgctat acgggcactg tgttcgtgcg ggggaaaaca ctgtccgcta
     3241 ttgaaattcg taccccagaa tcttgcaggt gttccatcct caaaaacaaa ttcattgcct
     3301 ctgacctgca ggaaaccatg cttgcccgca ggtttttccc cggcaaatat gaaggataag
     3361 tctaccggca tatcatccca tggcaggcta taagggatgg ttttccccgg ggaaatttcc
     3421 tgtgcggtca tatccgctgc agaacctgat tcgtttagcg taattgagga tgaaagaagc
     3481 aaaagtatga aaactgcaga aaaggatttc cctttttgca ggagcagcag gaacttactt
     3541 ttcccattgt gatcatacag tattctcctt ttcatagtat aaatatttat tgtttaggta
     3601 aaagtaccgg tctttgaatt ttggtttatt ctcaacctga tcattgaacc tgccaaagtg
     3661 aactgatcat ttccagggtt ttttcaacca gttcctgtcc gccttccggt cccatcagtg
     3721 tgcttgccgg gacagcgccg taggatcccc cggcgacgga gcgttgcgga ggtaaataag
     3781 atccgcttcc ggcaagctga acaagaaaag tctgtatggc aggactgcgc ccctttattc
     3841 tcattccgta atcgaggtag agttcgaagg gattcgtggc aatcaccatg tcgcctatcc
     3901 tcaggacatg aacctcaacc ggcatccggg gctgtatctt ctccagctca taccgttcct
     3961 ttacgctttc accccttttc atcagggtat ggatttgcga aataccggtg taccagcgtg
     4021 gtttcagggt ggcatcggga ttattccgga tttcacttat cagctcatta tattgctctc
     4081 tgtaagtttc tgattccctg agagcattat ttacatcagc tgtgtcaatg agtcttctcg
     4141 aaagctcaac gatttccatt ttgtgctcaa ataccggatc ccggtcaata ctgcttttca
     4201 tatacggcag tattgaggtg actgcatcgg ctattctcag ggcaatctgt ttcctgtgtg
     4261 ccagagtctg gccgccggtt atactgtcgg agaacatcag cttctgcatt cttgcctcag
     4321 ctttttcacc gatcaggaca tgcggcgact ggtctcccgc ggcactgcac tggggaagga
     4381 tatgtatttc atctccaagt cttccgcgaa gctcaatcct cgtctcgttc cagtaatcgg
     4441 cactgatcat gaacctgcct tcggtgacct gggccggaca agggacgttt accactatgc
     4501 cggtgagatt gttcctgtca tcccaggtat atagcaggtt cagggaatga tcctcatatc
     4561 cttccacatg actgaacccg ggagtgtcag gtcttttata catagttgat ctgccatcct
     4621 tgtcaacagt cagtctgttg tgacccacaa cggcatggcc cagcccaaaa cttattcccc
     4681 cggccctgcg gcttttccat gccttttccg cggcccgggc tatacgctct gcgatatatt
     4741 tctggcagtc ggcaggagac agaacatcaa gctcaacacc gtaaacgctt tccgaattcc
     4801 gggctgatcc gacaagcgga gctgtgtggg tatgtgtggc attgaggaag atctgttccg
     4861 gactgacttc aggaatggta tgcttcagga gctcccttac attatccctc aggttgtcgg
     4921 gggagccatc acgcatgccg tcactgatac ttaccagatc gcagctgatc ataatgattt
     4981 tcgctgaaga aggcccgctg ccgctctcaa gagcaagagc cgttgccgta atgggatcca
     5041 ttacaccctc catgacccgt gcatgaaatt gtccggctat gagcacaggt ttgtcgggtg
     5101 tgatgtcggc ggatgcccag ccgattttga aatttcctga atgctcatgt aaatccgctg
     5161 aaatcaggaa tactgcaatc acactcatta acagtgaccg ttcaaggaca ttacaaatat
     5221 gtttcatgat gatttcgttt gtttttgcct gaatacttca taagtttaaa aatacggata
     5281 aaacctgact cccgtgcacc tccggtgagt ttgccggaat catcaggcgc gacggatgtt
     5341 tctagcggcg ccgtatcact cttgtcttgt aaaggtctat gtcaggaacc gtaatggttt
     5401 tgtcaggatt cctggaatag atgttatgtg tccaccagct gatctctcct tttgtataaa
     5461 gcagttcccc cgtggattca ttgtagttga cggatttcac attcctgaca tccttaagca
     5521 attcaaattc acgaaattgt tcatcaggga tgtcaaaggt ccatactccg ttgtgtgtgg
     5581 ttattataag cctgtcgtcc gtaatgttat tcagatcgtg gccgccgtta tccggcaatt
     5641 gccatttcct gtccggttgc agtgagggat tgtcagtatc ccagtctttc agtgaatatg
     5701 accggagttc gtcaaagccc agggcaaaca atctttgata ccggggcatc cacaccactc
     5761 cgtgaccgga ataaaggctg tcctggtaaa ttaccttatc cggtttcccg atatcgaata
     5821 actgaatgca gtttccttcg ggatttgttg acaatgcaac gacaatcctg tcgcccggca
     5881 gtaattcagc cgagtgagcc atggggacat acgcataaaa caggcttttc tttgttttcc
     5941 tgtctgtcag aaccactccg cctgatgatg aagcaatgag tatcttttta ttgttgtcaa
     6001 ccggtttgca ttcatcggtg gttctcatat atttcaggta atccccgggc agatcgtcaa
     6061 tctcgcctat tttccacttc caggtaataa acacatttgt atcggccgac ttgtttctgt
     6121 caataattat tacctggtca tctccgcagg caattatttc atcagcagct tccgagcagg
     6181 ccggaaaaag aagaacagtc agaaaaggta aaaggaaggc aattcgtttc atagctttga
     6241 ttttttaaag tgacgcgaca agcttgattt cccgtgccgg atatgggtaa gcaaacccgt
     6301 tttcccgtaa agataaaaaa aaggaagaag agggaagttc tgcatgccgt atccggtgca
     6361 aaaggacctt gcaaagccag ccggaaataa aagtccggat ctgccgtatt gctgaacatt
     6421 tagctggtaa ttctgtttat ttactgatga gaaaagcact tgccatatta attacaggcc
     6481 tgatgttcct cactggaatg aggataagca tagaccacca ttactgcggc ggggaacttg
     6541 ctgccaggcg cttgtccctt acaggtacag cggcatcgtg cgggatggaa gatcagaaac
     6601 attccttctc atttcaaaca gaattcggca gtatttgttg tgaggatcag tttgcttcat
     6661 atggcataaa caatatattc atacctgaat acttcagact gacacatccg gacggcggca
     6721 aaaaagtccg tttttttcag gctccgcccc cgcatctgtc acctgcagtt tctgatcaga
     6781 ctgaaacggc tttccggaac aagcctcccg gtgagaagcc cgagtcagaa gtttccctgt
     6841 ccaggatctg cgttctgcgg atctgattgt ttgcctctct tcacccggct cgccggtaca
     6901 ggaaataatt gccggcccgg gtcccgatac catattgtta cgtcttatca ttaacactaa
     6961 agagaatcag agatgaaatc attcaatttt gcttttgcag tgttgctgat ggcaggtttg
     7021 agcatgtcgg ccgctgcaca gaccggtcag ccggaccgga cggacagatc agtccccggg
     7081 actgaatctg tcagaaccga atccttcagg gtatggggaa aatgcggtat gtgcaagaca
     7141 cgcattgaga cgactgtaaa aagtgaagga gcatccgctg catcatggga tcagaaaaca
     7201 cagttgctga ctgtaagcta tgatcctgcc aaaacaaaca gtgatgcgct caaaaaaaag
     7261 ctggcagccg ccggtcatga taccgaaaag tacaaggctc cggatgatgt ttattcaaag
     7321 cttccggcat gctgccatta tgaacgttca aaataggcag acaggtttcc gggctgacgg
     7381 ccggcgcggg atgtgtatta cattctttct gtaaatcaaa atgtttaaca ggctggtaaa
     7441 atattttctg gagaacaggg tggtagcctt tctgctgttc atcattttcg tggtggcagg
     7501 tctgattacc atgccttttg ataagaaggt ttcctttctg cctgaaagcc cg
//
LOCUS       DAOSPX010000150         7518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9173_length_7518_cov_4.531154, whole genome shotgun sequence.
ACCESSION   DAOSPX010000150 DAOSPX010000000
VERSION     DAOSPX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9173_length_7518_cov_4.531154"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PLZ75_08380"
     CDS             <1..922
                     /locus_tag="PLZ75_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HOS72447.1"
                     /translation="AGLASEISRTGKEAGFSVEIFSKGKIEALKMGGLLSVNRGSVDP
                     PAFCVLEWKPDSPVNSRPIVLVGKGVVYDTGGLNIKTGDYMAQMKGDMAGAAAVTGVF
                     HTIARNNIPLYLVGLIPVTDNRPGGNAYAQGDIITMYNKMTVEIGNTDAEGRLILADA
                     ISYASKYRPELIIDVATLTGSAAITFGNKAIAMMTNADRKYTDLLAECGNQVYERVAG
                     LPFYDEYKEAIKSEVADIRNIGGREAGAITAGKFLEHFAEFPIIHLDIAGTGILKKDD
                     HYRTKDGPGSGVRLLSVFLKRLSETNNKIK"
     gene            926..2032
                     /gene="pdxA"
                     /locus_tag="PLZ75_08385"
     CDS             926..2032
                     /gene="pdxA"
                     /locus_tag="PLZ75_08385"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOS72448.1"
                     /translation="MQEKSIIAGISHGDINGIGYEVIIKALMNTLINDLCVPVVYGSP
                     KVAAYHRKTLNINNFSFNNIRSAEDANPKRANMINCLDDNVRVELGKSTSQGGEAALV
                     SLERAVGDLKSGKIDVLITAPIDKNNIQSDRFSFKGHTEYLRSEAGSEDALMFMISEN
                     MRIGFATGHLPLRKVPDVITAELLVRKLRLMNQSLIIDFGIRRPRIAVLGLNPHAGDN
                     MLLGTEEAEIIIPAIKNAEQEGIMAFGPFPADGFFGSGTFSKFDAVLSMYHDQGLSPF
                     KALAFDSGVNFTAGLPFIRTSPVHGTAFSIAGKGEASENSFRQAMYLACSIYRNRQMH
                     EEISKNQLKHQDVDIHADKVDELPPEIYNSEQEI"
     gene            2029..2610
                     /gene="ruvA"
                     /locus_tag="PLZ75_08390"
     CDS             2029..2610
                     /gene="ruvA"
                     /locus_tag="PLZ75_08390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOS72449.1"
                     /translation="MMDYIKGIITGLTPAFLIIETGGIGYFVNISLNTFTRLEGKSDY
                     RILIHEVIREDLHQLFGFADKEERDIFRLLISVTGVGAGTARMMLSSLSPEEIEKAII
                     GSDVHKLKSVKGIGVKTAERIIVDLRDKLGKAAASGEIFSFTDNTRREEALSALVMLG
                     FARGTVLKTLDKIIREDRNLTVEEMIKKALKNL"
     gene            2743..>7518
                     /gene="sprA"
                     /locus_tag="PLZ75_08395"
     CDS             2743..>7518
                     /gene="sprA"
                     /locus_tag="PLZ75_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOS72450.1"
                     /translation="MAPDTTRRPVLRLQDVSGIPWENRSQNPLFLRNPSNITSEVIYN
                     PEKDEYIIYQKVGSFDYRTPVYMSPEEFRKYEFGRAMREYWHSRIAGDVSGYRSGLIP
                     QIQVGGEAFDKIFGSNIINIIPQGSAELIFSINISRTQNPTLSEKLRTIPTFDFKEKI
                     QMNVTGTIGDKMELGVNYNTDAMFEFENRTKLQYSGKEDEILKKVEAGDVTLPLSGTL
                     ITGSYSLFGLKTEMQFGKLTLTTVLSQQKGESSVIQVEGGAQQTRFEIFADDYEANRH
                     FFLSQYFRDIYDDALKNLPVISSGINIERIEVWITNKASRFEEGSNRNIVAFMDLAEN
                     GNHIFNNIPEFQQSPGAAIYPGNNANNLYEQLNTNYSGVRDVDQVTNVFDHLYPGFQI
                     GRDYEKIENARKLNEREYRVNRQLGYISLNTALNTDEVLAVAFEYTLNGKVYKVGEFS
                     TDGIVAPQALLLKLVKGTTLTPRLPTWQLMMKNIYSLGSGRLETRDFELNVLYQDDKT
                     GNAINYLPEGKLQDMILLQVMGLDNLNSQSDREADGYFDFIEGVTVMSDRGKIIFPVL
                     EPFGSHLKKKINDNILADKYIFQELYDSTQTIARQMAEKNKFMLAGQYRSESGSEIRL
                     NATNIPHGSVKVTAGGVTLSENTDYTVDYNMGTVRIINQALIESQTPIQVSLESNQFF
                     GFQTKTLVGTHLDYRFSDNFNIGGTILHLTERPYTQKVNFGEEPISNTIWGLNTSYKT
                     QSQLLTNIIDKIPLLHTRTPSSISFFGEFAQLVPGHSRAISSAGNSYIDDFESSEIPL
                     DLKSFNAWTISSVPQGQEQFFPEGRLNNNLASGFNRAKLAWYVIDPLFLRNGSSTPDH
                     IKRNPDLQSSHFVREIFENEIFPYRESPSGIPTNITVLNVAYYPDEKGPYNYDTYPGP
                     YSAGMNPEGRLNDPKSRWGGMMREVLTSDFEAANIQYVKFWLMDPFVENPGHEGGDLY
                     INLGNISEDILRDSRKSFENGLPASPVVTNVDTTAWGRVPTVQAVVHAFDNDPESRRY
                     QDVGLDGLGNEDETSFFSDYLEKVRDITKPEVYSEIEKDPSNDDFHYYRGSDYDFRQL
                     GILERYKRYNGMEGNSPTSEMSQESYPTSGSTLPDMEDINRDNTLSETESYYQYRVSM
                     RPEDIKVGSNYVVDEIEYEATMANGTKSRVKWYQFKIPITDYTKIIGPINDFKSIRFI
                     RMFMRGFSEPVILRFAELDLVRAEWRKYNISFMEGGERVTIPEASDGTFEISSVNIEE
                     NSGKEPVNYVLPPGFTRITDPTNPQLRQLNEQSMVLRVQNLEDGDARAAFKNVNLDIR
                     QYRRLRMEVHAEAMIGQPLADDELTAFIRIGSDYRSNFYEYEIPLKLTPPGRYDNKSD
                     ESRALVWPEANSFNIDLKVFQAAKQERNKEMMEHGSSLSVSDVFVYYHDGHRISVSGN
                     PNLSNVRVIMIGVRNPIRTRNPNRDDGNPKSGEVWVNELRLSDFIEEGGWAANAHLQA
                     RLADLGTVDMVGQTSTPGWGSIEKKVNERSKETIMKYDLSSTLEFGKFFPEKTGVRLP
                     VYVGYSETRIKPLYNPLDPDVFLEDALINAGDSFARDSIR"
BASE COUNT         2157 a         1628 c         1935 g         1798 t
ORIGIN      
        1 tgcaggactg gcttcagaga tcagcaggac cggtaaagag gccggattca gtgttgagat
       61 attcagcaag ggaaagattg aggctctcaa gatgggcgga ctgctttccg ttaacagggg
      121 aagtgttgat cctcccgctt tctgtgttct tgaatggaag ccggacagtc cggtgaacag
      181 cagaccgatt gtgcttgtgg gcaagggagt tgtatatgac acgggaggtc ttaacataaa
      241 gacaggcgat tatatggcac agatgaaggg tgatatggcc ggtgcagctg ctgttaccgg
      301 ggtattccat accattgcaa ggaataatat cccgctttat ctggtgggcc tgataccggt
      361 caccgataac cggccgggag gaaatgctta tgcacagggg gatataatca ccatgtacaa
      421 taaaatgaca gtggagatag gcaataccga tgcggaggga aggctgatcc ttgccgatgc
      481 catcagttac gcctcgaaat acaggcctga actgataata gacgtggcca cccttaccgg
      541 ttcggcagca atcacattcg gaaacaaggc catagccatg atgaccaatg ctgacaggaa
      601 atatacggac cttcttgcgg aatgcggaaa ccaggtttat gaaagagttg ccggactgcc
      661 cttttatgac gaatacaagg aggcaatcaa gtcggaagtg gcagatatca ggaatatcgg
      721 cggaagagag gcaggtgcga taactgccgg aaaattcctt gaacattttg ccgaattccc
      781 cattattcac ctggatattg caggaacggg aatactgaaa aaggatgatc attaccggac
      841 gaaggacggt ccgggctcag gagtaaggct tttgtcagtg ttcctgaaaa gattgtcgga
      901 aacaaataat aaaattaagt gacagatgca ggaaaaatcc attatagcag gaatttcaca
      961 tggcgacatc aacggaattg gttatgaggt tattataaag gcactcatga acactctcat
     1021 caatgacctg tgtgtgcccg ttgtgtatgg ttcgccgaaa gtggctgcgt accataggaa
     1081 aacattaaac atcaacaatt tcagtttcaa taacatcaga tcggctgagg atgcgaaccc
     1141 gaagagagcc aacatgataa actgtctgga tgacaatgtg cgggttgaac tggggaaatc
     1201 gacatcccag ggaggtgaag cagctcttgt ttcccttgaa agggctgtcg gggacctgaa
     1261 aagcgggaag atagatgtgc tgatcacggc gccgatagat aaaaacaaca ttcagtcgga
     1321 caggttcagt ttcaagggtc atacggaata tctcagatcg gaggccggtt cggaagatgc
     1381 tctcatgttc atgatcagtg aaaacatgag aataggtttt gcaacagggc atcttcccct
     1441 gaggaaggtt cccgatgtta tcacggctga actgctggtg cgcaagctga ggctcatgaa
     1501 tcagtcactc atcattgatt tcggcatacg caggccccgc attgctgttc tcggactgaa
     1561 tccgcatgcc ggcgacaata tgcttctcgg caccgaggaa gctgaaataa taatccctgc
     1621 gataaaaaat gctgaacagg agggaattat ggctttcgga ccttttccgg ctgacggatt
     1681 ctttggctca ggaacctttt caaaattcga cgctgtgctt tcaatgtacc acgatcaggg
     1741 attgtcgccc ttcaaggcat tggcctttga ttcaggagtt aattttactg ccgggctgcc
     1801 ctttataagg acatcacccg ttcacggcac ggcattttcc attgcgggaa aaggcgaagc
     1861 ctcggaaaac tcattcaggc aggcaatgta tcttgcatgc agcatataca gaaacaggca
     1921 gatgcatgag gagatttcaa aaaaccagct gaagcatcag gatgttgata tacacgctga
     1981 taaagttgat gaactgcctc cggaaattta taattccgaa caggaaatat gatggattat
     2041 ataaaaggaa taattaccgg gctcacacct gctttcctga taattgagac cggcggtata
     2101 ggatattttg taaatatttc acttaacacc tttaccaggc tggagggaaa gagtgattac
     2161 aggattctga ttcatgaagt gatcagggaa gatcttcacc agcttttcgg atttgccgat
     2221 aaggaagaaa gggatatctt ccggctgctc atatccgtaa caggggttgg tgccggcacg
     2281 gcaagaatga tgctttcctc attaagcccg gaagaaattg aaaaagctat catcggatct
     2341 gacgttcaca agcttaaaag tgtaaaaggg atcggggtga agactgcgga aagaataatt
     2401 gttgacctga gggataagct cggaaaagcg gccgcaagcg gtgaaatatt ttcatttaca
     2461 gacaatacaa ggagagaaga ggcgttatct gctttagtga tgctgggatt tgccagaggt
     2521 actgtcctta agacccttga caagataatc agggaggaca ggaacctgac tgttgaagaa
     2581 atgataaaga aggcgctgaa aaacttgtaa aacagaatat aatccgggtt gacttcaggg
     2641 aaagtatata gaaataacct cttatttgtt ttccttttat tgtctgtttc cttttttttt
     2701 agtttaagga aagcagacgg acaaatatct gccgggacat caatggcccc ggataccaca
     2761 agacgcccgg tactcaggct tcaggatgtt tcgggaatcc cgtgggaaaa caggagccag
     2821 aatccccttt ttctccgcaa tccttcaaac ataacatcgg aagttattta taatcctgaa
     2881 aaggatgaat acatcattta ccagaaagta ggatcgtttg actaccgcac tccggtttac
     2941 atgagtccgg aggaattcag gaaatatgaa tttggcaggg caatgcgtga atactggcat
     3001 tccaggattg ccggcgatgt ttcaggctac aggtcgggac tgataccgca gatccaggtg
     3061 ggaggcgagg ctttcgacaa gattttcgga agcaatatca tcaatattat cccgcagggc
     3121 tcggccgaac tgatattcag catcaatatc tcgcgcaccc agaatcctac tctttcagaa
     3181 aagctcagga ccattcccac attcgacttc aaggaaaaga tacagatgaa tgtcacggga
     3241 accataggcg acaagatgga gcttggtgtc aattacaata ccgatgccat gttcgagttt
     3301 gagaacagga ccaagcttca gtactcggga aaggaagacg agatacttaa aaaggttgaa
     3361 gcgggagacg tgacccttcc tctttccgga acccttataa cgggcagcta cagccttttc
     3421 ggactgaaaa cagagatgca gttcggaaaa ctcaccctta caactgtcct ttcacagcaa
     3481 aagggcgaat cttctgtgat ccaggtggaa ggaggggcac agcaaacccg cttcgagatc
     3541 tttgcggatg actatgaagc caacaggcat ttctttctct cgcaatattt cagggatata
     3601 tatgatgatg ccctgaaaaa cctgcctgtg ataagctcag gtatcaacat tgaaaggatt
     3661 gaggtgtgga ttacgaacaa ggccagccgc tttgaggagg gaagcaaccg taatatagtg
     3721 gcttttatgg acctggcgga aaacgggaat catattttca ataatattcc tgaattccag
     3781 caatctcccg gtgcggcaat ttatcccgga aacaatgcca acaatctcta tgaacagctg
     3841 aacacgaact acagcggtgt gagggatgtg gaccaggtga ctaacgtgtt tgatcatctt
     3901 tatccggggt ttcagatagg ccgtgactat gaaaagattg aaaatgcccg caagctcaat
     3961 gaacgggaat acagggtaaa caggcagctc ggatatattt cccttaacac agccctgaat
     4021 acggatgagg tgctggcagt tgcattcgaa tacaccctta acggaaaggt ttacaaggtg
     4081 ggggagtttt ccaccgacgg aatagttgca ccccaggccc tgctgctgaa gctggtcaag
     4141 ggaaccaccc ttactcccag gcttcctacc tggcagctga tgatgaagaa catctattca
     4201 ctcggctcgg gccggctcga aacgcgcgat tttgaactga atgtcctgta tcaggatgat
     4261 aaaaccggta acgccatcaa ttatcttccg gaagggaagc tccaggatat gatactgttg
     4321 caggtaatgg gactggacaa cctgaattcc cagtcggaca gggaagcgga cggatatttc
     4381 gactttatag aaggcgtgac cgtgatgagt gacaggggaa agattatttt cccggttctt
     4441 gagcctttcg ggagccatct gaagaaaaag ataaatgaca acatacttgc ggataaatat
     4501 atttttcagg agctttatga ttccacccag accattgcgc ggcagatggc agagaagaac
     4561 aagtttatgc tggccggcca gtacaggtct gaatcaggat cggagatccg cctgaacgct
     4621 acaaatatcc cgcacggctc cgtaaaagta acggccggag gcgtaactct gagtgagaac
     4681 actgactata cggttgacta caatatggga acggtaagga ttatcaacca ggctcttatt
     4741 gaatcccaga ctcccattca ggtatccctg gaaagcaatc agttctttgg tttccagaca
     4801 aagacgctgg tgggtacaca tcttgattac cgcttctcgg acaatttcaa tatcgggggt
     4861 accatactgc accttaccga aaggccctat actcaaaagg ttaattttgg ggaagagccc
     4921 atatcaaata ccatctgggg actgaatact tcctacaaaa cccagtcgca gctgcttaca
     4981 aacattattg acaagattcc gctccttcat accagaactc catcatcaat ttcattcttc
     5041 ggagagtttg cacagctggt acccgggcat tccagggcga tatcaagtgc gggcaattcc
     5101 tatattgacg attttgagtc aagtgaaata cctcttgatc ttaaatcctt caatgcctgg
     5161 actatttcca gtgtacccca gggccaggag cagttctttc cggaaggacg gctcaacaat
     5221 aacctggcaa gcggtttcaa cagggcaaag ctggcctggt atgttattga tcccctgttt
     5281 cttcgaaacg gctcttcaac tcctgatcac atcaagcgga atcccgatct tcagtcgagc
     5341 cattttgtaa gggagatctt tgagaatgag attttcccct atcgtgagag cccgagcggc
     5401 atacccacca acatcaccgt gctgaatgtg gcatattatc ctgatgaaaa ggggccctac
     5461 aactatgata cctatcccgg gccatattcg gctggcatga atccggaagg caggctcaat
     5521 gatccgaaat cgcgatgggg cggcatgatg agagaggtgc tgacaagtga ttttgaggcg
     5581 gcgaacattc aatacgtaaa attctggctg atggatccct ttgtggagaa tcccgggcac
     5641 gaaggaggag acctttatat aaaccttgga aatatttcag aagatattct gcgtgattca
     5701 aggaaaagct ttgagaacgg cctgcctgca tcgcctgttg ttaccaatgt ggacaccacg
     5761 gcctggggaa gggtgcctac cgttcaggcg gttgtccatg cctttgacaa tgatcccgaa
     5821 tcaaggcgtt atcaggatgt cggactggac ggactgggca atgaggatga aacatcgttc
     5881 ttttccgatt atcttgagaa agtaagggat atcaccaaac cggaagttta cagtgaaata
     5941 gaaaaggatc cctccaatga tgattttcat tattaccggg ggtcggatta tgatttccgg
     6001 caactcggaa ttcttgaaag atacaagagg tacaacggca tggagggaaa ctcccccaca
     6061 tccgagatgt cgcaggagtc atatcccaca tcgggttcaa ccctgcctga catggaggat
     6121 ataaaccgcg acaataccct gagcgaaacg gaaagctatt accagtacag ggtcagtatg
     6181 aggccggagg atattaaggt gggatcgaac tacgtggtgg atgaaattga gtatgaagcc
     6241 acaatggcaa atggtacgaa atcgagggtg aaatggtatc agttcaagat tcccattacg
     6301 gattacacta agattatcgg acccatcaat gattttaaat ccatccgttt tataaggatg
     6361 ttcatgagag gcttcagcga gcctgtgata ttacgctttg ctgaactcga cctggtgcgg
     6421 gctgaatgga ggaaatacaa tatttccttc atggaagggg gtgaaagggt taccataccg
     6481 gaagcctccg acggcacatt cgagatcagt tccgtaaata ttgaggagaa ctccggaaag
     6541 gagccggtta actatgtgct tcctccgggt tttacccgta ttactgatcc gacaaatccg
     6601 cagctgcgac agcttaatga gcagtcgatg gtgcttcgtg tacagaattt ggaagacggt
     6661 gatgcccgtg ctgcattcaa gaatgttaat ctggatatcc ggcagtacag gcgcctgagg
     6721 atggaagttc atgccgaagc aatgataggc cagcctcttg ctgatgatga actgactgcc
     6781 ttcatccgta tcggttcgga ttacaggagc aatttttatg aatatgagat tcccctcaag
     6841 cttaccccgc cggggcgcta tgacaataaa tccgatgaat caagggcact tgtctggccc
     6901 gaggcaaaca gtttcaacat agacctcaag gtttttcagg ccgcaaagca ggagaggaat
     6961 aaagaaatga tggagcacgg ttcctccctg agcgtctcgg atgtttttgt atattatcat
     7021 gacggccaca ggatttctgt ttcgggtaac cccaacctca gcaatgtcag ggtgatcatg
     7081 ataggtgtta ggaatccgat aaggacaagg aatcccaaca gggatgacgg caatccgaag
     7141 tccggcgagg tctgggtaaa tgaactcagg ctgagtgatt tcattgaaga gggaggctgg
     7201 gctgccaatg cgcatctgca ggcacgcctg gctgacctgg gaactgttga catggtcggc
     7261 cagacgagca cgcccggctg gggatcaata gaaaagaagg taaacgagag aagcaaggaa
     7321 accataatga aatatgacct gtcgtcaacc ctggagttcg gcaagttttt ccccgagaaa
     7381 acaggagtga gactgccggt ttatgtggga tattcggaaa ccaggataaa acccctttac
     7441 aatcctctcg atcccgatgt tttccttgag gatgcactga taaatgccgg agacagtttt
     7501 gcgcgggatt cgatcagg
//
LOCUS       DAOSPX010000151         7512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9179_length_7512_cov_4.146440, whole genome shotgun sequence.
ACCESSION   DAOSPX010000151 DAOSPX010000000
VERSION     DAOSPX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9179_length_7512_cov_4.146440"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLZ75_08400"
     CDS             <1..345
                     /locus_tag="PLZ75_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS72451.1"
                     /translation="QRYLSEKRMPVGKLIENADHSFPLILLDHQPLQLGEAADNGIDL
                     QLSGHTHKGQLWPLNYLIDKIYEVGYGHRKIGNTHVIVSSGFGLWGPRVRTSGRSEVI
                     QVDLEFTGSGQN"
     gene            complement(354..1643)
                     /gene="murF"
                     /locus_tag="PLZ75_08405"
     CDS             complement(354..1643)
                     /gene="murF"
                     /locus_tag="PLZ75_08405"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOS72452.1"
                     /translation="MTEQIYQLFRESKGLSTDSRTISEGQMFLALWGNKFNGNKYAAD
                     ALKKGASWAVIDDPAFETEKTILVDDTLMELHKLATHHRKELKCPVLAVTGSNGKTTT
                     KELIAAVLSKKYRVHSTKRNLNNQIGVPLTILSAQEGTEIMVLEMGASHIGEIRTLCL
                     IARPDYGIITNIGAAHMEGFGSIEGVAKAKTELYEHLRKVNGIAFYNDRDRLISSKIY
                     GMVNRAVPFSDPAGTALKAVPEITGLNLAVNVKYRKKKLKVQTKLFGAYNADNVRAAM
                     AVGLFFGVDIEDIADAIENYEPEDNRSRVIVTENNTLISDSYNANPVSMKLALESFAA
                     IKAENKLCILGDMLELGDRTEEEHKKIHRVLTDHNLQNVMLTGPVFTKVSAGFRFKTF
                     SSVGRLRKYLKLKPVKGYHILIKGSRGMALEQIYDVL"
     gene            complement(1712..3202)
                     /gene="gldJ"
                     /locus_tag="PLZ75_08410"
     CDS             complement(1712..3202)
                     /gene="gldJ"
                     /locus_tag="PLZ75_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HOS72453.1"
                     /translation="MCRIKFLPVILIAGSVLVSCKGGKQANTSPTTGWRYNSPDHGGF
                     EVVLGAEQKTGPGLVLIEGGRFTMGQVQQDVLFDWNNKPRTVTVSSFYMDETEITNVD
                     YREYLYWLRRVYVDYPEVYRRALPDTLVWRSPLGFNDPYVQYYFRHPAYNTYPVVGVN
                     WLQANDYCLWRSDRVNEQILIDEGELKPDPEQKNQNNFNTEAYLVGQYEGVVNRLRES
                     LNPNQTERRTTFEDGVLLPNYRLPTEAEWEYAAYGLRGNTYQERIYERRIYPWDGHNV
                     RNAKKRHRGQMMANFVRGRGDMMGVAGSLNDNASITADVKSYWPNDYGLYCMAGNVNE
                     WVLDVYRPLSSDDVDAFNPFRGNVYTDLRRDANGNVVTKNNLGRLYIDTVKDIDVANR
                     YNYQKGDYRNYRDGDLQSSITSDWRADDGMRGSERMYAQSTGAEGASDYVTLINDNAR
                     VFKGGSWKDRAYWLNPSTRRFLDQESCRDDLGFRCAMIRVGSPAGF"
     gene            complement(3280..4248)
                     /locus_tag="PLZ75_08415"
     CDS             complement(3280..4248)
                     /locus_tag="PLZ75_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOS72454.1"
                     /translation="MTKQLIGITFLIIITGGISGGQYSESGFDYQAIMLNNPALAGAE
                     GDGSLRLSFINHYPGQGYKLFSGRMSYDSYFPSLHGGVGIFVSNDYLGGIINDLQGGF
                     SYAYYFRAGRDVFISAGLSASVWHRGYNFSSAVLPEQIDPAGNIVFPGSETVSQNGSY
                     MLDLGTGFLFMTERIFWGISVNHLTEPDVDYSGDQAGKTGRKLLVHAGGDFHISREKE
                     FRIRPLVKGEITKDYFSAGAGAVLEMANLSLSTVLLGDGERNTDLQAGFALNTPGMKL
                     FYNYRFNIATGKNLLPFSLLHHVGIVLGLNSVDKRRVIKTINYPAL"
     gene            4400..>7512
                     /gene="porU"
                     /locus_tag="PLZ75_08420"
     CDS             4400..>7512
                     /gene="porU"
                     /locus_tag="PLZ75_08420"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOS72455.1"
                     /translation="MAFIPAILYSQSSIENYSASSVLSSGKWLKIAVTADGIYKISYS
                     QLKEAGLSDPSGPRLFASNWGQLSYLTGDPAPDDLREISVLLSTGSDGIFGEGDYLLF
                     YGQATHRWKYNEGEGRYDFVRHNYSDTAFYFITSGGGAARTISAAAVHTEEPDYYSSE
                     SDLLFIHELETENIIKSGREWYQPVSSLTPVEIRPEFTDLVSTGEIRYRIRVLARAPS
                     VSQFSFSEGESLLNTILVQDVNMYNYTGTYARISDSSGTVLPLSATPVYRIGFRNEKD
                     PGARGWLDFVTLHARKHNVYRGKTLHVLDSRTVSAGNLTEYSVNSPGRNPLVWDITDA
                     GNPKNVNPARSGENIVFRANSDSLRKFVIFSESDALKPVFRPGPVANQDLHASPPAEM
                     VIVTHPLFRKYAKKLADLHFEREGLVSQIVTPGEIYNEFSGGIPDIAAIRNFLRMKYL
                     RQKNTATPLRYLLLFGDGSYENKTPPPNSPNYIPTYQSQNSNIIVSSFTSDDFYGLLE
                     DGKGEAEGTEDIGIGRIPVTDTLQAGIVVNKIRRYLESSNTGEWKNIVCMVADDEDGN
                     AHMADAEGLSLLLQDSIPEINVDKIYLDAFRQETSAAGQSYPDVTIAINNRINSGCLI
                     FNYVGHGNENGLAHERVVKTEDINSWKNGTKLPLFITATCEFSRFDDADINMATKIIS
                     PRTSAGEKVLLNPGGGAIALMSTTRVAYSAPNYYLNRNIYDAIIRLRESGEPLALGDI
                     MRIAKNNSGDGPNKRNFSLLGDPAVILAFPPHGKVITDSINHGPAGSAADTLKALSTI
                     TISGHIEDHGGTLIDDFNGTVSQVIYDKPAIIRTLANDGGPPMTFEMRNNILFSGKTK
                     AANGRFSFTFMVPRDINYSFGTGKISYYAKDETRDMNGSYSGIIVGGFENTAASDTAG
                     PLIKLYLNDTLFRSGGISCPNPRILAIIEDEGGINTTGAGIGHDITAYLDNDPASAII
                     LNNYFESDFNSHTRGRISYALSDLPEGNHTLTLKAWDNFNNSSSKTISFTVTPEEKFM
                     LRDIVVYP"
BASE COUNT         2034 a         1898 c         1647 g         1933 t
ORIGIN      
        1 cagaggtacc tgtcagaaaa aagaatgccg gtcggaaaac tcattgaaaa tgccgatcat
       61 tcatttccgc ttatactgct ggaccatcag cccctgcaac tgggtgaggc agcagataat
      121 ggcattgacc ttcagctgtc cggccatacc cacaaaggac agctgtggcc tctcaactac
      181 cttattgaca aaatttatga ggtaggctac ggacacagga aaatagggaa tacccatgta
      241 atagtctctt cgggttttgg tctgtgggga ccacgggtga gaacatccgg aagatccgaa
      301 gtgatacagg tggatctgga atttaccggc agcggacaga actgagttcc ggatcacaat
      361 acatcataaa tctgctcaag agccattccc ctggagcctt ttataagaat atggtatccc
      421 ttcacaggtt taagcttcag atatttcctg agcctgccga cgctcgagaa tgtcttgaac
      481 ctgaatccgg cggaaacttt cgtaaatacc ggacccgtca gcattacatt ctgaagattg
      541 tgatccgtaa gcacccggtg tattttttta tgttcctcct ccgtcctgtc cccgagctca
      601 agcatgtcgc ccagaatgca caatttgttt tctgccttta ttgccgcaaa tgattccagg
      661 gcaagcttca tgcttaccgg gttggcattg tatgaatcgc tgatcagggt gttgttctcc
      721 gtgacaatca cacgcgaacg attgtcttcc ggttcatagt tttcaattgc atcggcaata
      781 tcctcaatgt caacaccgaa aaacagtccc accgccatag ctgccctgac gttgtcagca
      841 ttataggctc cgaacagctt tgtctgaacc ttcagtttct ttttacggta cttcacgttt
      901 accgccaggt tcaggccggt aatctcagga actgctttca gtgccgtccc tgccggatcc
      961 gagaagggaa ccgccctgtt gaccattccg tagatctttg agctgatcag tctgtccctg
     1021 tcattgtaga aggctatgcc gtttaccttg cggagatgtt catagagttc cgtttttgct
     1081 ttcgcaaccc cctcgattga tccgaaacct tccatatgtg ccgcacctat attggtaatg
     1141 atcccgtaat cgggccgggc tatgagacag agtgtcctga tctcgccgat atggctggcg
     1201 cccatctcaa gtaccatgat ctcagtgccc tcctgtgctg ataatatggt gaggggcacg
     1261 ccaatctgat tattgagatt cctttttgtg gaatgaaccc tgtacttctt tgaaagaact
     1321 gccgcgataa gctccttagt cgttgtcttt ccgttggaac ccgtaactgc cagaaccggg
     1381 catttcagtt ccttcctgtg atgtgtcgca agcttatgca actccatcag cgtgtcatcc
     1441 accagaatgg ttttttccgt ttcaaaggcc ggatcatcga taacagccca tgaagccccc
     1501 ttcttaaggg catcagcagc atacttattg ccgttgaact tatttcccca gagggcaagg
     1561 aacatctgcc cttccgaaat ggtccgcgaa tctgttgaca agcctttgga ttccctgaaa
     1621 agctgataaa tctgctctgt cattatattt tcaaaaaaat gccccattat gatatcataa
     1681 tggggctaaa gatagtttta tttttttaca atcagaaccc tgcaggactt cctactctga
     1741 tcattgcgca tctgaatccg agatcatcgc ggcagctttc ctggtcaagg aaacgccgtg
     1801 tggaaggatt gagccagtat gccctgtctt tccaggaacc tcccttgaaa accctggcat
     1861 tatcatttat cagcgtgaca taatcactcg caccttcagc tccggtactc tgggcataca
     1921 ttctttcaga ccccctcatg ccgtcatctg cacgccagtc acttgttatt gaggactgca
     1981 ggtcgccatc cctgtagttc ctgtaatctc ccttctggta attatacctg ttggccacat
     2041 caatgtcctt tacggtatca atatacaaac ggccgagatt gttttttgta accacattgc
     2101 cgtttgcatc acggcggaga tcggtataca cgttgcccct gaaagggttg aatgcatcaa
     2161 cgtcgtccga tgaaagcgga cggtacacat ccagaaccca ttcgttcacg ttgccggcca
     2221 tgcaatagag gccataatca ttgggccagt aggatttcac atcggctgtg atgcttgcat
     2281 tatcgttaag acttcctgcc acacccatca tatctcccct gccccggaca aagttcgcca
     2341 tcatctggcc gcggtgacgc tttttggcat tccttacatt atggccgtcc catggataaa
     2401 tccttctttc ataaatcctt tcctggtatg tattgccccg cagaccatat gcagcatatt
     2461 cccattcggc ttcagtggga aggcggtagt tgggaagaag cactccgtct tcaaaagttg
     2521 tccttctttc agtctgattg ggattgagac tctctctcag ccggttcact acaccttcat
     2581 actgcccaac aaggtatgct tcggtattga aattgttctg attcttctgc tcgggatcag
     2641 gcttcagctc accttcatca ataaggatct gttcattgac ccggtcactc ctccacaggc
     2701 agtaatcatt ggcctgcagc cagttaacac cgacaaccgg ataggtgtta tatgccggat
     2761 gacggaaata gtactgaaca tagggatcat tgaagcccag gggacttctc catacaagcg
     2821 tatctggcaa agccctcctg tatacttcgg gataatcaac atacacacgc ctcagccagt
     2881 aaagatattc acggtaatca acgttggtta tctcggtttc atccatataa aaagatgata
     2941 cggtgactgt gcgcggcttg ttgttccagt caaacaaaac atcctgctga acctgtccca
     3001 tggtgaacct gcccccttct ataaggacaa gcccgggacc cgttttctgt tcagcaccca
     3061 aaaccacttc aaaaccaccg tgatcaggac tgttatacct ccatccggtg gtgggagatg
     3121 tattggcctg tttgcctccc ttgcatgaca cgagaacaga accggctatc agaattaccg
     3181 gcaagaattt tattctgcac atattactat ggtttttttg acttttatca gtttacaaag
     3241 ataataattt ccaaaaatta tttcaaacaa taagtataac tataatgcag ggtaatttat
     3301 tgtttttatg actcttcttt tatcaacact gtttaaacca agcactattc ccacatggtg
     3361 cagaagcgag aacggaagca gatttttccc cgttgcaata ttaaaacggt aattgtaaaa
     3421 caacttcatt cccggggtat tcagcgcgaa tccggcctga agatctgtgt tcctttcccc
     3481 gtcacccagc aaaactgtac ttaacgacag attcgccatt tcaaggaccg ctcctgctcc
     3541 cgccgagaaa taatccttag taatctcacc tttaaccagt ggtctgatcc tgaattcctt
     3601 ttccctgctg atatgaaaat cccctcctgc atgaaccagc agcttcctgc cagttttccc
     3661 cgcctgatca cctgaataat caacatccgg ctccgtcaga tggttcactg aaattcccca
     3721 gaaaatcctt tctgtcataa aaagaaatcc agtacccagg tccagcatgt aactgccgtt
     3781 ctgtgatact gtttcacttc ccggaaaaac aatattcccg gcaggatcta tctgctcagg
     3841 cagtaccgca ctgctgaaat tatagcctct gtgccacacc gaggctgaaa ggccggcact
     3901 tataaataca tccctgcccg cccggaaata ataggcgtag gaaaatcctc cctgtaaatc
     3961 attaatgatt ccacccagat aatcgttcga gacaaaaatc ccgacacctc cgtgcaggga
     4021 agggaaatag gaatcgtatg acattcttcc cgaaaaaagt ttgtaaccct gtccgggata
     4081 gtgattaatg aatgacagtc tcagcgatcc gtctccctca gctccggcca gtgcgggatt
     4141 attaagcatg atcgcctgat aatcaaaacc gctttcagag tattgcccgc ccgagattcc
     4201 ccctgtgata ataatcagga aagtgattcc aataagctgt tttgtcataa tattgcaagc
     4261 tgcaatatca acgtttttaa agcgttatta ttttaatttt gcctggctgt aaacaataaa
     4321 aaaacaagtc aaagaacatt ataaatttgt gtctttgtac gtaatgatgt tcagattgtt
     4381 gcataatata tcacttatat tggcttttat acccgccatt ctgtattcac aatcatccat
     4441 tgaaaactat tctgcatctt cggtcctctc atcagggaaa tggcttaaaa ttgcggtgac
     4501 agcagacggt atctataaga taagctattc acagctgaaa gaggccggtc tgagcgatcc
     4561 ttccggccca aggctctttg ccagtaactg gggccagctt tcatacctca ccggcgatcc
     4621 ggcaccggat gacctcaggg agatttcagt actgctttca accggaagcg acggcatctt
     4681 cggtgaaggt gattatctgc tcttttacgg acaggcaacc cacagatgga agtataatga
     4741 aggcgaaggc agatatgatt ttgtcaggca caattactcg gataccgcct tctatttcat
     4801 aacatcggga gggggagccg ccagaacaat atccgctgct gcggtccata cggaggaacc
     4861 ggactattat tcatcagaat cagatcttct tttcatccat gaactggaaa cagaaaatat
     4921 tataaaatcc ggacgggaat ggtatcagcc ggtttcttca cttacgcctg ttgaaatcag
     4981 gcctgaattc acggatctgg taagtaccgg cgaaatcaga tacaggataa gggtactggc
     5041 aagggctccc tcagtttcac aattcagctt ctcggaaggg gaaagcctgc tgaacacaat
     5101 ccttgtgcag gatgtgaaca tgtacaatta tacgggaacc tatgcccgta taagcgattc
     5161 ctcgggaacg gtgctgcctt tgtcggctac accggtttac cggataggat tcaggaatga
     5221 aaaggacccc ggcgccaggg gatggctgga ctttgtgact ctgcatgcaa gaaagcacaa
     5281 tgtttacagg ggaaaaacac ttcatgtcct cgactcaagg actgtctcag cgggaaacct
     5341 gactgaatat tccgtgaaca gtcccggcag gaatcctctt gtatgggata ttacggatgc
     5401 aggcaatccg aaaaacgtga acccggcaag atcaggggaa aatattgttt tcagggccaa
     5461 ttctgattca ctgaggaaat ttgtgatctt cagcgagtca gatgcactga agcccgtttt
     5521 caggccgggt cccgtggcaa accaggatct tcacgcttcc ccacccgcgg aaatggttat
     5581 agtaactcat cccctgttca gaaaatatgc aaaaaagctt gctgatctgc attttgaaag
     5641 agagggactt gtttcacaga tagtaacgcc cggggagatc tataatgaat tctcgggagg
     5701 cattcccgat atagctgcca tcaggaattt tctccggatg aaatatctca ggcagaaaaa
     5761 cacagccacc cctctgagat acctgctcct tttcggcgac ggctcatatg agaataaaac
     5821 gcctcccccg aacagcccca attatattcc tacctatcag tcacagaatt caaacatcat
     5881 tgtctcttca ttcacctctg acgatttcta cggcttgctt gaagacggga agggtgaagc
     5941 cgaaggaacc gaggatatcg gcatcgggcg cattcccgtg acggataccc ttcaggctgg
     6001 tattgtggta aacaagataa gaagatacct tgaatcatcc aacacgggtg aatggaagaa
     6061 catagtatgc atggttgccg acgatgaaga cggcaatgct catatggcag atgcggaagg
     6121 tctcagcctg ctccttcagg acagtattcc ggaaataaat gtggacaaaa tctatctgga
     6181 tgcattcagg caggagacct cggcagccgg ccagtcctat cccgacgtaa caatagcaat
     6241 caataaccgt ataaattccg gttgtctgat cttcaactat gtcggccacg gaaatgaaaa
     6301 cggactcgct catgaaagag tcgtaaaaac cgaagatatc aattcctgga agaacgggac
     6361 aaagcttccc cttttcataa cggccacatg tgaattcagc agattcgacg acgctgatat
     6421 aaatatggcc acaaagatta taagtccgag aacatcggcc ggcgaaaagg tactgctgaa
     6481 tcccggcggg ggggcaatag cactgatgtc cacaacacgc gttgcctatt cagccccgaa
     6541 ctattacctg aaccgcaata tttacgatgc gataatcagg ctgagggaat ccggtgaacc
     6601 actggctctc ggcgatatca tgcggattgc aaagaataat tcgggagacg gacccaataa
     6661 gaggaacttt tccctgctgg gcgaccccgc ggtaatactg gcatttcccc ctcacgggaa
     6721 agtaattacc gactcaataa accatgggcc tgcaggcagt gctgccgata cccttaaagc
     6781 actctcaaca ataaccatat caggtcatat cgaggatcac ggaggaaccc ttattgatga
     6841 tttcaacgga acggtgtcgc aggtgatcta cgacaagccc gccataatca ggacactggc
     6901 aaacgacggc ggaccgccga tgacctttga aatgaggaac aacattcttt tcagcgggaa
     6961 aacaaaggcg gcaaacggcc gattcagctt taccttcatg gttccgagag atattaacta
     7021 ttccttcgga acaggaaaaa taagctacta cgcaaaggac gaaacacgcg acatgaacgg
     7081 cagctattcc ggaataatcg tcgggggatt cgagaatacc gccgctagcg acactgccgg
     7141 accgctgata aaattatatc tcaatgacac tcttttcaga agcggaggaa tcagctgccc
     7201 gaatccaaga atactggcaa taattgagga tgagggaggg ataaacacca cgggggcggg
     7261 aatcggtcac gacattacag catatctcga caatgacccc gcttcagcaa tcatcctgaa
     7321 taattatttt gaaagtgatt ttaacagcca tacgagggga agaatcagct atgccctgag
     7381 tgatctgccg gaaggaaacc atacccttac ccttaaagcc tgggataatt tcaacaattc
     7441 ttccagtaaa accatcagct tcaccgttac accggaagaa aaattcatgc tcagggacat
     7501 tgtcgtttac cc
//
LOCUS       DAOSPX010000152         7475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9241_length_7475_cov_4.221563, whole genome shotgun sequence.
ACCESSION   DAOSPX010000152 DAOSPX010000000
VERSION     DAOSPX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9241_length_7475_cov_4.221563"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLZ75_08425"
     CDS             complement(<1..875)
                     /locus_tag="PLZ75_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOS72456.1"
                     /translation="MKKSKKILKTALPVLFIMGLTVIILITWFKGRRIETEITMSDGT
                     ALSTILFIPGGGGRFPAVMIRTPYNKYNEEWIGRAFSIFRIAVVIQDVRGKFKSEGEF
                     CPFLNERKDGLETIRWIRKQSWSDGTVAGWGASYLGYTQWAVSDSLDFITPLLSGARL
                     YDLFYPDSLFSLQTAGIWGFEVASDSPNSLDEDIIRKNLFTLPVIEADDVTIRDIAFY
                     NDWVMHEEYDSYWKQMDFRGRTAAHVLSVAGWYDLFLQCQIADFQALDSSGNNRGRLV
                     IGPWAHGEPGEASQYG"
     gene            complement(978..1943)
                     /locus_tag="PLZ75_08430"
     CDS             complement(978..1943)
                     /locus_tag="PLZ75_08430"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOS72457.1"
                     /translation="MKKRIFSGSLLAAILIMGMVSCKKDPPTCDFTYTADGLKVTFTS
                     VVSNANTYAWDFGDGNTSTEANPVHHYGGAGDFEVRLTVTGDGGTAEKKIKVTVIPGL
                     EDYKIMLTGGPGAPNGKTWVLNTASVIDGDGASAIEPNMLVLLPIPPDFYGWMGRERL
                     DGLKDEFTFKYDGSYIIDPKNDTIIAVSLFAFVNGITAEDSNTPYGTCRATPYVQPPG
                     ATWEIKETNFTVDAITNPADQTVPPAHKEVTFSGKKWLHFSQGSYLGLLDFTTSSNII
                     IKSISPAQLRVAMIVCMYQGAGHPGGIVYASMPTYMYHMTFIPKP"
     gene            complement(2052..3524)
                     /locus_tag="PLZ75_08435"
     CDS             complement(2052..3524)
                     /locus_tag="PLZ75_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS72458.1"
                     /translation="MMMFRISKHFFAVSGLLLIPLLLGSGISSSGRAGEPWDDGPNQP
                     AGTPSGIHPGRVVWAWNPDATNENCRSTFETGDWYWKPENTNETIVGTMFREALGKLS
                     GDSSVTGSWDRLFHHFNMMKKGTARGYIQGEKIFIKIIQGQSRWILTQDEKDQGYYLS
                     ESSGDSGSKRRHSWTPVETGPYIVLELLRELVGEMGINEKDIAIADPFAHIYGHNYDV
                     WSKEFPDVMYCDRSSADHGRTLIKPTEKNMVHFSDNSFSDKIYDVVEKADYTINVSNL
                     RMHGAALISLTAKNHLGSITSPTAKLLHYTHLSRSKEGAAYEGYFKYRALVDIMGSSY
                     LGQNTILSIVDALFGGGSDETRGPVKYFMSPFNYDWCNSIFLSQDQVALESVCYDFLR
                     AEWDGVNRHDPHNNEWEIIPDRNGIDDYLHQAADPANWPEGLSYDPDGSGHPLKSLGV
                     HEHWNDPVKKQYSRNLGKNTGIELISIPDSLVRCESAPEL"
     gene            3977..5119
                     /locus_tag="PLZ75_08440"
     CDS             3977..5119
                     /locus_tag="PLZ75_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HOS72459.1"
                     /translation="MHRKRLLPLFAAGLVLFITACSTSTKQKEKPLIIGYVGGFNRNI
                     IETSGIDPHKLTHINYAFVDVKDHRAWLTNEETDTVNFRLLNGLKKINPDLKILISVG
                     GWTWSKNFSDAVLSDTSCRNFAQSAVDIALKYDLDGVDIDWEYPGMIGDGNVYRPEDR
                     ENYTRLFRDLREIFNEAAAQTGKEYLVTTAIGGSREFLQHTQLEIAQHYLDYIMLMAY
                     DFDGTYDNMSSHHSNLYSPPNMPWIYSGDVCIKNMMAVGVDPSKIVLGIGFYGKGRVV
                     KSTENNGLYQIPVPVRRWLGGGYSYLKDSLVNRKGYVRYWDDVSKAPYLFNAEEKAFI
                     TYDDEESTRLKCHYILDNNLAGIMFWEYFSDWKHYLLDVINEVFSE"
     gene            complement(5216..5746)
                     /locus_tag="PLZ75_08445"
     CDS             complement(5216..5746)
                     /locus_tag="PLZ75_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72460.1"
                     /translation="MKIPNAVSRLILIALVAAIAGQLMAQTQEKKEDKTVWGRNIVYN
                     PQPPPPVPPAENNEGRQPKDMLKQITYLDGEIVPGAYYAECSWLYKAYPDLVWVEEHT
                     HDFDEVFGLYGSDPENPEELNGEIELWIGGEKHLITRSCLIFIPKGVKHCPLILRRID
                     RPVFFFTTGPSRKYEF"
     gene            complement(5778..>7475)
                     /locus_tag="PLZ75_08450"
     CDS             complement(5778..>7475)
                     /locus_tag="PLZ75_08450"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOS72461.1"
                     /translation="RIPFSSLKFKPSDDVTTMGLIICRNISANNETDTYPLIDPRFGM
                     IASNKPSLAQEIRIEGVKPARPVYVSPYLLGGYSRSYDLNDLQSGYVRKDIPQYNAGL
                     DIKYNINSNLTLDLTANTDFAQVEADDQQVNLTRYSLFFPEKRKFFQERSSLFDFSLG
                     GFFDNMFYSRSIGVHNGSPVRIYGGARITGRLGKWDTGFLDMQTAAHDTLPGENFGVL
                     RMRRQVINQNSYVGGIITSRMSMNGYQNFGYGVDGIFRLFGVDYLNVKWAQTYDSEIG
                     NRMNSLDPSFIMIDWERRNEKGLAYRFNYTYSGEQLRPGTGFIMRGNVQGLNGRLAYG
                     WLPGEKSRLYNYSINLDAEQYKRLDDGKLESARISPGFDIGTKKGINAGFSVEIQKEG
                     VRHDFFLSENVIIRAGEYTFTKAQLMLFTPNSKLISLMGSASAGRFYDGSRYGTMTML
                     NFNISSGLNLSVNYDFNAIRFPDRASDNSLNIHLVNAKALMMFSTRLSATLIVQYVNT
                     YGDLLTNFRLRYNPKEGNDLYLVFNDNRSLSQKESGPEYPRFFNKTAMLKYVHTFSL"
BASE COUNT         1977 a         1698 c         1685 g         2115 t
ORIGIN      
        1 ccgtattgac tggcttcacc cggttcgccg tgtgcccatg gaccaatgac aagcctgccc
       61 ctgttgttgc cggaagagtc gagagcctga aaatctgcta tctgacattg aagaaacaga
      121 tcataccagc cggctacgga aagaacatga gcagcggtcc tgcccctgaa gtccatttgt
      181 ttccagtagc tgtcatattc ctcatgcatg acccagtcgt tataaaaggc aatatccctt
      241 attgtgacat catctgcttc gataacaggc aaagtgaaaa gatttttcct gattatgtcc
      301 tcatcaaggc tgttaggact gtctgaggca acttcaaagc cccatattcc ggccgtctgc
      361 agtgagaaca gggagtccgg atagaataag tcatacaacc ttgcaccgct cagcagtggc
      421 gtaataaaat cgagagaatc cgaaactgcc cactgggtgt agcccaaata gctggcaccc
      481 cagcctgcca cagtgccatc agaccaggac tgtttcctga tccagcgaat ggtttccagc
      541 ccgtctttcc tctcattaag gaagggacag aattcacctt ccgacttgaa ttttcctctg
      601 acatcctgta ttacaactgc tatcctgaaa atgctgaatg cccgtcctat ccattcttcg
      661 ttatatttat tgtacggagt gcggatcatt accgcaggaa atctgcctcc cccgcccggt
      721 ataaaaagaa ttgtgctgag agctgtaccg tcactcatag tgatttctgt ttcaattctg
      781 cgtcctttaa accaggtaat cagtattatt actgttaagc ccataatgaa aagaacaggc
      841 agtgctgttt taaggatctt tttggatttt ttcatcagag gtggtatgat cagaaaggaa
      901 tcataaaaaa acagcccggt ataccgttcc tgtttaacgg ctcccgggcc gggcaatgat
      961 tttgaataat tcagaaatta cggttttggt atgaatgtca tatggtacat gtatgtcggc
     1021 atacttgcgt atactattcc tcccgggtgg cctgcaccct gatacatgca tactatcatt
     1081 gccaccctca gctgtgcagg ggagattgac ttgataataa tattgctgga tgtcgtaaag
     1141 tcaagcagtc ccagatagga accctgggag aaatggagcc attttttgcc tgaaaaggtg
     1201 acctctttgt gggcaggcgg cacagtttga tctgccggat ttgtgattgc atcaacggta
     1261 aaattagttt cctttatttc ccatgtggct ccggggggct gaacataggg ggttgccctg
     1321 caggtaccgt aaggagtatt gctgtcttca gcagtaatgc cattgacaaa tgcaaacaga
     1381 gaaactgcta tgatagtatc atttttagga tcaataatgt atgatccgtc atacttgaaa
     1441 gtgaactcat ctttcaggcc atcgagcctt tcgcggccca tccatccgta gaagtcaggc
     1501 gggataggaa ggaggacaag catgttgggc tcaattgcac ttgccccgtc gccatcaatc
     1561 acgctggcag tattcagtac ccaggtcttg ccgttcgggg cacccggtcc tccggtcagc
     1621 attattttgt aatcttcaag acccggtatg acggtcactt ttatcttttt ttcagctgtt
     1681 ccgccgtcac ctgtaacagt cagcctgacc tcaaaatcac cggcacctcc ataatgatgt
     1741 accgggttgg cttccgtact ggtattcccg tcgccgaaat cccatgcgta agtgtttgcg
     1801 ttgcttacaa cagaagtaaa tgtaaccttt aatccgtctg cagtatatgt gaaatcacat
     1861 gttggaggat cctttttgca ggataccatt cccataataa ggatggcagc cagcaggctt
     1921 ccggaaaata ttcttttttt catggcataa atggttggtt tgtattctat taagcaaata
     1981 tatgcttaaa cgtttaaaga tgcaagtttt ttgagacttt tttaacccct accggttgaa
     2041 aggctgtcct gtcaaagttc aggtgcagat tcacaccgga ccagactgtc aggtattgag
     2101 atcagttcaa tacccgtatt ttttcccaga ttgcgggaat actgcttttt aacaggatca
     2161 ttccagtgct catgtacccc cagacttttc agcggatgtc cgctgccgtc agggtcatag
     2221 ctcaagccct ccggccaatt ggcaggatca gcagcctggt gaagataatc atcaataccg
     2281 tttctgtcag ggattatttc ccattcattg ttatggggat catgcctgtt gaccccgtcc
     2341 cattccgccc gcaggaaatc gtagcatact gattccaggg caacctgatc ctgagacagg
     2401 aagattgaat tgcaccagtc gtaattgaaa ggagacataa aatattttac cggtcccctg
     2461 gtttcatccg agcctccgcc aaacagggcg tccactatgc tgagaatggt attttgtccc
     2521 agatagctgc ttcccatgat gtcgacaagt gcacggtact tgaaataacc ttcataggca
     2581 gcaccttctt tgctccttga gagatgggta tagtgaagaa gcttggcagt gggacttgtt
     2641 atgcttccaa gatggttttt tgccgtgagg gaaataaggg ctgcgccgtg cattctgagg
     2701 ttggaaacat ttatcgtata atctgctttt tcaaccacat cgtaaatctt gtcggagaaa
     2761 ctgttatccg agaaatgaac catgtttttt tccgttggtt ttatcagggt acggccatga
     2821 tctgccgaac ttctgtcgca atacatcaca tcaggaaact ctttcgacca gacatcgtaa
     2881 ttgtgtccgt aaatatgggc aaaagggtca gctatggcaa tatccttctc gtttattccc
     2941 atttcgccga cgagctccct gagtaattca aggactatat acggtccggt ttcaaccgga
     3001 gtccagctgt gccttctttt tgaccctgaa tctccgctgc tttcggaaag atagtatccc
     3061 tgatccttct catcctgggt aagtatccat ctggactggc cctgaatgat ttttataaag
     3121 atcttttctc cctggatata gcccctggca gttccttttt tcatcatgtt gaaatggtga
     3181 aagagcctgt cccaggatcc tgtaacgctg ctgtctccgc taagtttccc gagggcctca
     3241 cgaaacattg tccctacaat agtttcattt gtattttccg gtttccagta ccagtcgccg
     3301 gtttcgaaag tggatctgca gttttcattt gttgcatccg ggttccatgc ccataccacc
     3361 ctgcccggat gtattcctga aggagtgccg gcgggctgat tgggcccgtc atcccatggt
     3421 tccccggccc gtccagaaga agaaatccct gatcccagaa gaagcgggat aagcaaaagg
     3481 ccacttaccg caaaaaaatg tttagaaatt ctgaacatca tcataatgta ttgtataagc
     3541 agttgatctt aaattacgtt gatcctttct tctgaatttt cagttccgct atccggtctg
     3601 gattttttcg gaaaaggatg cagggcaggc aaaattattc aaatttttca ataaacagct
     3661 cggcccggtg aattgaatag aacagaacgg aggtttgaga ataaaacctg aaaaaagaaa
     3721 acggaaaaaa tcctggttcc tgcaagggac ttgcagcatg atatacaaaa gtatagcatt
     3781 attttggctg attgcaaaca aattctatct ttgagagaat ttctgaagta tgtcgctgca
     3841 ggaatttcag atccggctta acatgagtgg ataaatcagg cggggatact tattttgccg
     3901 ggattaataa aatcggaaat aattattgaa ttcattcaca tacagaatca ttaaattgtt
     3961 tttaaaaccg aaaaagatgc acagaaaaag attattgcca ttatttgcag caggcctggt
     4021 tctctttatt acagcctgca gcacctcaac aaagcagaaa gaaaagcctc tgatcattgg
     4081 ttatgtcggg ggattcaaca ggaacattat tgaaacctcg gggattgacc ctcacaagct
     4141 tacccatata aactatgctt ttgtcgatgt caaggaccac agggcatggc ttaccaatga
     4201 ggaaaccgat acagtcaatt tccgattatt gaacggactg aagaaaataa accctgatct
     4261 gaaaatcctg atctctgtcg gaggatggac ctggagcaag aatttctccg atgctgtttt
     4321 gtcagacacc tcctgcagga attttgctca gagcgctgtt gatattgctc taaaatatga
     4381 tcttgacgga gttgatattg actgggaata tcccggaatg attggggacg gcaatgttta
     4441 ccgtcctgaa gacagggaaa attataccag gctgttcagg gacctgcgcg agatatttaa
     4501 tgaagcggca gcccagacgg gaaaggaata cctggttaca acagccattg gcggatcaag
     4561 ggaattcctg cagcataccc agctggaaat agcccagcat tatcttgatt acataatgct
     4621 catggcctat gattttgacg ggacctatga taatatgtca tcacaccatt cgaatctgta
     4681 ttctcctcca aacatgccgt ggatctattc cggtgacgtc tgtataaaaa atatgatggc
     4741 tgttggcgtt gacccttcaa aaatcgtgct gggaataggc ttttacggaa agggaagagt
     4801 tgtgaaaagc actgaaaaca atggtctgta ccagataccg gtaccggtaa ggagatggct
     4861 aggagggggg tactcctatc tgaaggacag ccttgtaaac agaaagggct atgtcaggta
     4921 ctgggatgat gtatcaaagg ctccctatct gttcaatgcc gaggaaaagg catttattac
     4981 ctatgatgat gaagaatcaa ccaggttgaa atgtcattat atcctggaca ataacctggc
     5041 cggcattatg ttctgggaat atttcagtga ctggaagcat tacctgctgg atgttataaa
     5101 tgaagttttc agcgaatgat aattatatat cctgccgggc ctgtctgcca gctccggcag
     5161 gattatacag taaatatcaa tcctgcagac ggttctgcag gtttttattt ataacctaga
     5221 actcatattt acgggacggg cctgttgtaa agaaaaaaac cggtctgtca attctccgga
     5281 gaatgagagg gcagtgcttc acaccctttg ggatgaaaat caggcagctt ctggtaatca
     5341 ggtgcttttc gccaccaatc cacaattcaa tctcaccatt cagttcctca ggattttcag
     5401 gatcgcttcc ataaaggcca aagacttcat caaaatcgtg agtgtgttct tcaacccata
     5461 ccagatcggg ataggccttg tacagccagg aacattctgc ataataggct cctggcacga
     5521 tctcaccgtc gaggtaggta atttgcttta gcatatcttt tggctgtctg ccttcattat
     5581 tttcggcagg aggaacaggc gggggaggct ggggattgta aacaatgttt ctgccccaaa
     5641 cggttttgtc ttcctttttt tcctgagtct gagccatgag ctggcctgca atggctgcca
     5701 caagagctat caggataagc cgtgaaactg cattgggaat tttcatgatc tgaagtttaa
     5761 atgtgaatta atagggacta caggctaaat gtatgaacat acttaagcat ggctgtctta
     5821 ttgaaaaacc tgggatattc aggtcctgat tccttttgtg acaggctgcg gttatcattg
     5881 aaaacaagat acaggtcatt tccctcttta ggattgtacc tcagcctgaa attggtcagg
     5941 agatcgccgt aagtgttgac atactgcact atgagtgttg cagataacct ggtgctgaac
     6001 atcatcaggg ccttagcatt taccaggtgg atattcagcg aattatcgga agccctgtca
     6061 gggaaacgga ttgcattgaa atcataatta acagacaggt tcagaccgga agaaatattg
     6121 aaattcaaca ttgtcatggt tccatacctg ctgccgtcat aaaaacggcc cgcactggca
     6181 ctgcccataa gggaaatcag ttttgaattc ggggtgaaca gcataagctg cgcttttgta
     6241 aaagtatatt ctcccgccct gatgattaca ttttcggaaa ggaaaaagtc atgcctgact
     6301 ccttccttct gtatctcaac agaaaatccg gcatttattc ctttcttggt acctatgtca
     6361 aaacccgggc tgattcgtgc ggattcgagc tttccgtcgt ccaggcgctt atattgttcc
     6421 gcatcaagat ttatgctgta gttgtaaagc ctggattttt caccggggag ccatccgtat
     6481 gccagcctgc cattcagtcc ctgaacattt cccctcatta tgaatccggt acccggtctg
     6541 agctgttcac cggaatatgt atagttgaac ctgtaggcca gacctttttc atttctcctt
     6601 tcccaatcta tcataataaa tgaagggtca agggaattca tcctgtttcc gatttcactg
     6661 tcataggttt gtgcccattt aacattcagg taatcaaccc cgaaaaggcg gaatatgccg
     6721 tccactccgt aaccgaagtt ctgatagccg ttcattgaca tccgtgacgt gattatcccg
     6781 ccaacgtagc tgttctggtt aataacctgc cggcgcattc tgagcacgcc gaaattttca
     6841 ccgggaagtg tatcatgcgc tgcagtctgc atatccagaa aaccggtatc ccactttccc
     6901 agccttcccg ttatccttgc tcctccgtag attctgaccg gcgatccgtt atgcacaccg
     6961 atacttctgc tgtaaaacat attgtcaaaa aatccgccga ggctgaagtc aaacagactt
     7021 gacctttcct ggaagaattt tcttttttcc gggaaaaaga gggaataacg cgtcaggttt
     7081 acctgctgat catcagcctc aacctgggca aagtcggtat ttgccgtaag atcgagcgtg
     7141 agattgctgt taatgttgta tttgatatcc aggccggcat tatactgggg aatatccttt
     7201 ctgacatacc cgctctgtaa atcattcaga tcataactgc gggaatagcc tcccagcagg
     7261 taaggtgata catataccgg ccttgcaggt ttcacgcctt caatccggat ttcctgcgca
     7321 agggaaggct tgttggatgc tatcattcca aatctgggat ctataagcgg ataggtgtcc
     7381 gtttcgttat ttgcactgat attcctgcat ataatcagac ccattgtggt aacatcgtct
     7441 gaaggtttaa atttgaggct ggaaaaggga attct
//
LOCUS       DAOSPX010000153         7439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9288_length_7439_cov_4.755146, whole genome shotgun sequence.
ACCESSION   DAOSPX010000153 DAOSPX010000000
VERSION     DAOSPX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9288_length_7439_cov_4.755146"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="PLZ75_08455"
     CDS             complement(<1..1358)
                     /locus_tag="PLZ75_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOS72462.1"
                     /translation="MKYLFIFFLPFMILQTSLSQQITISGKVTDANKIPLAGVTVVAD
                     GTLIGTYTDSEGEFKLTVDRKAARSITCSYVGYERQTRRLEDGLLHYEFILASSMTEL
                     EDIIVIGYGTQRKDDITVSISSVATRDLTSPAITSLDQSLQGQAVGMVVSQSSGKPGA
                     PVSIRIRGTTSINGTNEPLYVVDGIPIITESQELTTGTIQGSDINPLISINPQDIESM
                     QVLKDASATAIYGARGANGVILINTRRGKRGSLQANLNLSYGIQKLSKKLDLLNAREL
                     AELGNDAVTEARKYYPEIAYGSNFAVPSRFGEGTDWQDQIFRTAPLMNGQLSVQGGTD
                     NTQYFMSANVVRHLGIIHNSDFNKGTVRINLDSDLSEKVRTGIGINLSHAINNGVTTG
                     VPNVASSVTAMALLFNPGQEVYDSTQAGGYTYESNTINRIPNPVAEINETEIVVTTDR
                     LI"
     gene            complement(1534..2415)
                     /locus_tag="PLZ75_08460"
     CDS             complement(1534..2415)
                     /locus_tag="PLZ75_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72463.1"
                     /translation="MKKTVLFICSIVLTGIISSQLIISCKKEKSVPTEMTINITSTPK
                     LNIGEVVNAKVTIVTDGVKSFKYYKIVDNKKDAGTEVAAQLVQTGKTWVYDFSYTVQQ
                     YDDLYTLGFEFELTDDKGEVKNVGLVVNINISVESMLVKYDWKVTESTWLGMDVLQPW
                     DAAIIYRFNKDGSYQEDLSPEYAADNHHFCFWVFKETPNNGDTISILRLVRRLKQGDT
                     AIDEYYDYKITAANESEMTMFWDVAVFGLLGIQNTFKSQAKGAFVPYGTPAEEAKVNA
                     NASLSCSHIDPALLIIN"
     gene            complement(2689..3318)
                     /locus_tag="PLZ75_08465"
     CDS             complement(2689..3318)
                     /locus_tag="PLZ75_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOS72464.1"
                     /translation="MKRLFLISVLVMAITLNGFSQFSFSVSPGIATNSATFGYKLGNA
                     VPYAGFQLVNGGFMREEVDHYQSGSDWVTNKHDVKANVNLLMPSLGLKYFAVQSGDLK
                     AYVNLAATKPLVSGKLKFDGDEEEEFAEQLKNIKLLGAELGFGAEYFLSKNFSIGGEY
                     SFRYISGKHSDKEDMGGGEYSKTDISLRLVPTIAKFSLNFYFGGGGDNN"
     gene            complement(3586..5727)
                     /locus_tag="PLZ75_08470"
     CDS             complement(3586..5727)
                     /locus_tag="PLZ75_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOS72465.1"
                     /translation="MKKAVYLFNAALIMLMSSCGKKPQAPVAEKIPYVVKSNGNERVD
                     NYFWMRLSDDQKNAETPDDQTARVLSWLNAENDYAGTVMKHTLSLQEKLFSELKGRIR
                     EDDETVPYLDNGYYYHTKYFSGKEYAVYYRQKTGSDSPEVMLDVNLLAEGHEYTGVAG
                     TTVSPDNRLLAFGTDHVSRRQYTLQIKDLASGELLPDNIPNTAGLALWADDSKTFLYV
                     SKDPETLRAATVMMHRLGTPVSEDRIIYHEGDETFSVQLGRSKSKKYIIIVSTHTLAT
                     EVRLVEAAGPEKGAVIFEPRGEDHLYSVDHLNDRFYIRTNSGGAINFRLMSCPENRTA
                     MTHWKDIIPHRPDILLGNFEIFDNYLVVNERIKGLTNLRIIDLENNSGHYLDFGEETY
                     TAGISINPNAGTSLLRYSYSSLTTPNSVYDYDMSSRTRTLLKEDEVLGGFDRNNYESR
                     RLWATAGDGTKVPVSLVYRRGFICDGNAPMLLYAYGSYGISSDPSFRSNIISLLDRGF
                     VYAIAHIRGGSEMGRQWYEDGKLLKKKNTFTDFADCARYLINENYTSARHLYAMGGSA
                     GGLLMGAVANMEPELFNGIIAQVPFVDIVSTMIDASIPLTTFEWDEWGDPRKQEYYDY
                     MLSYSPYDQVKEQAYPNILVTTGYWDSQVQYWEPAKWVARLREMKTDDNILIMDVNMA
                     AGHGGASGRFERLKTTALEYAFMLDLEGIKK"
     gene            6051..6932
                     /locus_tag="PLZ75_08475"
     CDS             6051..6932
                     /locus_tag="PLZ75_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOS72466.1"
                     /translation="MVMRKIIENVRTYVVTSFGLLLLSVAWTAFLLPHQISGSGVTGI
                     GAIVFYATGIPMGYTYFAINVVLMAIALKILGAGFGIKTIYSVVLSSVLLTLLQPLIP
                     APVVQEKFMSAIIGGMLCGAGLGIVFTQGGSTGGTDIIALVINKYRNISPGKIILLCD
                     VFIISSSYVVLTHLESAQRVETMVYGFVTMAIQAYTLDAILSGNKQSVQIFIFSRHFE
                     SIADLITSRMHRGVTVIDGTGWYTKESQKVIITMVRKNEVSEVYRIIKSVDKKAFITV
                     ANVMGVYGKGFEELKRL"
     gene            7026..>7439
                     /locus_tag="PLZ75_08480"
     CDS             7026..>7439
                     /locus_tag="PLZ75_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS72467.1"
                     /translation="MRKTVLIPVFFLAATSFLLSQQATRWRGPNAEGIYPGTGLLRSW
                     PENGPEMLWSFEQLGEGYASPSISGGYIYIPTMIKGEGYMFKLSISGKEIWRVKYGSE
                     FTRQYPGTRSSATIAGDLLYMLSGNGRLVCMDTGDG"
BASE COUNT         1937 a         1675 c         1739 g         2088 t
ORIGIN      
        1 ctgatcagcc tgtcggtggt aacaactatt tctgtttcat ttatttctgc aaccggattc
       61 gggatcctgt tgatggtatt gctctcgtag gtatagcctc cggcctgtgt tgaatcataa
      121 acctcctgac ccggattgaa cagcagtgcc attgcagtga cgctggatgc cacatttggc
      181 acacccgttg ttacaccgtt atttattgca tgtgacaggt ttattccaat acctgtcctg
      241 accttttcgg aaagatcact gtcaagattg attctgacgg ttcccttgtt aaaatcggaa
      301 ttatgtatta tacccagatg cctgactaca tttgcagaca tgaagtactg cgtattatcg
      361 gttcctccct gaacagagag ctggccgttc atcaggggag cagtccggaa tatctgatcc
      421 tgccagtcag ttccctcgcc aaaacggctg ggaacagcaa aattactgcc atatgctatt
      481 tcaggataat acttccttgc ttctgttacg gcatcatttc ccagttctgc cagttccctg
      541 gcattgagca ggtcaagttt tttcgataat ttctggatgc cataggaaag gttcagattt
      601 gcctgaagac ttcccctctt tccccttctg gtgttaatga ggattacgcc attggctccc
      661 cttgcaccgt atatcgctgt tgccgaggca tccttgagaa cctgcatgga ctcaatgtcc
      721 tgggggttaa tacttatcag gggattgatg tcggaaccct gaatagtacc tgtggtaagt
      781 tcctgtgatt ccgtaattat gggtatgcca tcaaccacat acaaaggttc gttggttccg
      841 ttgatggaag tggttcctct gatcctgatt gaaacaggtg cacccggctt tcccgaagac
      901 tggcttacaa ccattccgac ggcctgaccc tgcagggact gatcgagtga agtaattgcc
      961 ggtgaggtaa ggtcgcgtgt ggcaaccgac gatattgaca ctgtgatatc atccttgcgc
     1021 tgtgtaccgt atccgataac aataatatct tccagttcgg tcatactgga tgccaggatg
     1081 aactcataat ggagcaggcc atcctccagc cgccttgtct ggcgttcata acccacatag
     1141 gaacaagtga ttgatctggc agcttttctg tcaaccgtca gtttgaactc accttctgaa
     1201 tctgtatatg ttccgatgag agttccatct gcaacaacag ttacgccagc cagggggatc
     1261 ttgttcgcat cggtcacctt tccgcttatt gttatctgtt gcgacaagga agtctggaga
     1321 atcataaaag gcagaaaaaa aataaaaagg tatttcattg gatgggagta taactaatga
     1381 taaaaggaaa aaaaaagata tatcgcggag cactcttgct ctttcgcaat ggtttcagtt
     1441 gtattcctgc caagcgcata cagttcccgc ctgcccgaag gcataattat tgcccggtac
     1501 aggtaccggg cccagactgc agttatttta taattaattt attatcagta atgcaggatc
     1561 tatatgactg catgaaagag atgcatttgc attcaccttt gcttcctctg caggtgtgcc
     1621 gtatggtaca aacgctccct ttgcctggct cttgaaagtg ttttgtatcc ccagaagtcc
     1681 gaatactgcc acgtcccaga acattgtcat ctccgattca ttggctgccg tgattttgta
     1741 atcatagtac tcgtcaattg ccgtgtctcc ctgtttcagt cttctgacaa gtctcagaat
     1801 ggagattgta tcaccgttat tgggggtttc cttgaagacc cagaagcaga aatgatgatt
     1861 gtcggctgca tactccgggc tgagatcttc ctgatagctg ccatccttgt tgaaccggta
     1921 aataatggca gcatcccatg gttgaagaac atccatgcca agccaggtgc tttccgtaac
     1981 cttccagtcg tatttaacca gcatggattc cactgatatg ttgatattta caacaaggcc
     2041 cacgtttttg acctctccct tgtcatctgt cagttcaaat tcaaatccca gggtatacag
     2101 atcatcatac tgctggacag tatatgaaaa atcataaacc caggtttttc ctgtctgaac
     2161 aagctgggcg gcgacttcag taccggcatc tttcttgtta tcaacaatct tgtagtattt
     2221 aaatgatttc acgccgtcag tcacgattgt aaccttggca tttaccactt caccaatatt
     2281 tagtttaggg gtggatgtaa tattgattgt catttcggta ggtacgcttt tttccttctt
     2341 gcaggagatt atcagctggg aggaaataat gcccgtcagg acaatgctgc aaatgaataa
     2401 tacagttttt ttcatagccg taaattttaa atccgtaaca tttagtttaa taacataggt
     2461 ttcaggttta ttgagagaca agatagtaaa aaaatctaaa aaaagatgca cttttttaaa
     2521 aaagaagccg gcggaggtaa acagggttgc ctctgtatga agtgataagt taattggaat
     2581 tgtgtgttcc ggcagtatgg cagggagccg gagaagtatg catacaaaaa aaccggcctg
     2641 aaagaccggt tttcctgtgt ttacaactat atctggtgtc tttgctgctt agttgttgtc
     2701 tccgccgcca ccgaaataaa agttaagact gaattttgca attgtgggaa cgagtctgag
     2761 ggaaatatcg gtttttgaat attcacctcc tcccatatct tccttatcgg aatgttttcc
     2821 actgatataa cggaaggaat attcaccacc aatgctgaaa tttttgctca ggaaatattc
     2881 tgcaccgaat cccagttcag ctcccagcag ttttatgttt ttcagctgtt ctgcaaattc
     2941 ttcttcctca tcgccgtcaa atttcagttt accactgacc agtggcttgg tggctgcaag
     3001 attaacatag gccttgagat cgccggactg aacggcaaaa tatttcagac ccagtgaagg
     3061 catgagaaga tttacatttg ccttcacgtc atgtttgttt gtaacccagt cggacccgga
     3121 ttgataatga tctacttcct ccctcatgaa tcccccgtta accagctgaa aacctgcata
     3181 gggaacagca tttcctaatt tatagccaaa agtggctgaa tttgttgcaa tgcccggtga
     3241 cacgctgaaa gagaactgac tgaagccatt taatgttatt gccataacca gcacggagat
     3301 taagaataat cttttcattt taattggttt taattgttaa ctgaaataat ttgtttcaca
     3361 agtaacagga tctgtactga cttttcaggc atgatacgga caagcccgta aaatgttaca
     3421 gccgactggc ggtattaata atggcaggat ccggccggtc cggctgaatt ttcggaattt
     3481 ttgcgcggga ctctttcggg ggatatataa ataattgcag atccggaaga tagtcactgt
     3541 attcctttac cggagagtac aaacaagagg gcagagtgaa aattattatt tttttatccc
     3601 ttccagatca agcatgaagg catattcgag tgcggttgtt ttcagccgtt caaaacggcc
     3661 tgacgcaccg ccgtggcctg cagccatatt gacatccatg atgaggatgt tatcatccgt
     3721 tttcatctca cgcagcctgg caacccattt tgccggttcc cagtactgca cctgtgagtc
     3781 ccagtagccc gttgtgacaa gtatgttggg ataggcctgc tcctttacct ggtcgtaggg
     3841 agaataggaa agcatatagt cataatactc ctgtttgcgc ggatcgcccc actcatccca
     3901 ttcgaatgtg gtgagcggga tgcttgcatc gatcatggtg cttactatat caacaaaggg
     3961 tacctgtgct attatgccgt taaaaagttc cggttccatg ttggcgacgg cacccatcag
     4021 aagtcctcct gcacttccgc ccatggcata caggtgccgg gctgaagtat agttctcatt
     4081 tatcagatag cgggcacagt cggcaaagtc ggtgaaggtg tttttcttct tcagcagttt
     4141 gccgtcctca taccattgcc ggcccatttc actgcctccc ctgatgtggg ctatcgcata
     4201 aacgaagccc ctgtcaagga ggctgatgat attggaacgg aatgaaggat ccgaggaaat
     4261 gccgtatgat ccgtaggcat aaagcagcat gggcgcattg ccgtcgcaaa tgaatcccct
     4321 gcggtaaaca agcgatacag gaacctttgt tccgtcaccg gccgtggccc agagccgtct
     4381 cgattcataa ttgttcctgt caaagccgcc taggacttcg tcctccttaa gaagagtcct
     4441 tgtacgggaa gacatatcat aatcatacac ggaattaggc gtggtaagcg acgagtaact
     4501 gtatctcagg agggaggtgc cagcattggg attaatactt attcctgcag tataggtttc
     4561 ttcgccaaag tcgaggtaat gtccggaatt attttcgaga tcaatgatcc ggaggtttgt
     4621 aagacctttg attcgctcgt ttacaacaag atagttatca aaaatttcaa aattcccgag
     4681 gaggatatcg gggcgatgcg gaattatatc cttccagtgg gtcatggcgg tcctgttctc
     4741 ggggcaggac ataagcctga aatttatcgc tccccctgaa ttggtccgga tataaaacct
     4801 atcgttcagg tgatcaaccg agtacaaatg atcctccccg cgcggctcaa atattacggc
     4861 tcccttttcc ggaccggccg cttccacgag acgaacctcg gttgcaagag tatgggtgga
     4921 aacaataatg atgtattttt tactcttgct tctgccaagc tgaacggaaa aagtctcatc
     4981 cccctcatga tagatgattc tgtcttccga caccggggtt ccaagcctgt gcatcatcac
     5041 ggtggccgcc cggagtgttt ccggatcctt gctgacataa aggaatgttt tgctgtcgtc
     5101 agcccataag gccagtccgg cagtattggg aatattgtcg ggaagcagtt cgcccgaagc
     5161 gaggtctttg atctgcaggg tatactgcct ccggctgaca tgatccgttc cgaatgcaag
     5221 aagcctgttg tccggagaga ctgttgttcc ggcaactcca gtatactcat gtccttctgc
     5281 aagaagattc acatcaagca tcacttcagg agagtctgaa ccggtttttt gcctgtaata
     5341 cactgcatat tcctttccgg agaagtattt tgtatggtaa taataaccgt tgtcaagata
     5401 cggaactgtt tcatcatctt cccggatacg ccccttcagt tcactgaaga gcttttcctg
     5461 aagggataag gtatgcttca tgacagtgcc tgcgtagtca ttctccgcat tgagccagga
     5521 aaggacccta gcagtctggt catcaggtgt ttctgcattc ttctgatcat cactgaggcg
     5581 catccagaaa taattgtcaa cacgctcgtt tccattactt ttaacaacat aaggaatttt
     5641 ttctgcaacc ggggcctgtg gtttttttcc gcatgaggac ataagcataa tcaaggctgc
     5701 attaaagaga taaacagctt ttttcatgaa tataagcatt ttatcagtaa gtgtttgttt
     5761 cttgttattc tgaatgcaat ataaaaaacc ttgcggacat aatgattctg acggaaaaat
     5821 gagggaaaaa ggataagccg gacggatacc gtggaggctc attcaggcct ggctgccatc
     5881 aaaaaagagc ctgcaggaaa aaagctccgg tgctatactt cctgcagggg aaaacatttc
     5941 cggaaaaacg gcaagatggg aaaggcagtt ccggatcagt tggcccgtat atatcttttg
     6001 gaggtgttac agctgcacta tacctttgcg gaataaacaa gacaaggctt atggtaatgc
     6061 gcaaaatcat tgaaaatgtc aggacatatg ttgtgacatc atttggtctg ttgctgttgt
     6121 ctgtagcatg gaccgccttc ctgctgcccc atcagatatc cggcagcggc gttacgggca
     6181 ttggtgccat tgtcttctat gccacaggga taccaatggg ctatacctac tttgcaataa
     6241 acgtcgtact gatggcgata gcgctgaaga tactgggggc aggattcggc attaaaacca
     6301 tttactcagt agttctgtcg tctgttctgc tcaccctgct tcagccgctc atcccggcgc
     6361 ccgttgttca ggaaaagttt atgtcggcaa tcatcggggg catgctttgc ggagccggcc
     6421 ttggcattgt ctttacccag ggaggcagca cgggcggaac ggacatcatt gcactggtga
     6481 taaacaaata ccggaatata agtccgggca agatcatcct gctttgcgat gtattcatta
     6541 tcagctcctc ttatgtggtg ctgactcacc ttgaatccgc acagagggtg gaaacaatgg
     6601 tgtacggttt tgtgaccatg gcaatacagg cctatactct tgacgcaata ttgtccggca
     6661 acaaacagtc ggttcagata tttatttttt caaggcattt tgaaagcata gccgatctga
     6721 ttacttcccg gatgcataga ggggtaaccg ttatcgacgg taccggctgg tacacgaagg
     6781 agagtcagaa agtgataata accatggtaa ggaaaaatga agtaagcgag gtatacagaa
     6841 taataaaaag tgttgataaa aaggccttca tcaccgtggc taatgttatg ggtgtttacg
     6901 gaaaaggctt tgaggaactg aagaggttat gaaatgaagg tgacgggaca gaaccggaaa
     6961 cccgcaggca ttctttcatt ttacttaata ttatagatat attgcagcaa tttcaaaatg
     7021 agtacatgcg aaaaacagtt ttgataccgg tgttttttct ggcagcaaca agctttctgc
     7081 tatcccaaca ggccactaga tggagaggac ccaatgccga aggtatatat cccggcaccg
     7141 gactgctccg gagctggcct gaaaacggcc ctgaaatgct atggtcattc gaacagctcg
     7201 gggagggtta tgcttcacca tctatttccg ggggttacat atatattccg accatgatta
     7261 agggagaggg ctatatgttc aagctaagca tatcgggaaa agagatctgg agagtaaaat
     7321 acggcagtga attcacccgc cagtatccgg gaaccaggtc atcagccacc attgccggag
     7381 acctgctgta tatgctcagc ggcaatggca ggctggtatg catggataca ggggacgga
//
LOCUS       DAOSPX010000154         7427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9308_length_7427_cov_4.186381, whole genome shotgun sequence.
ACCESSION   DAOSPX010000154 DAOSPX010000000
VERSION     DAOSPX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9308_length_7427_cov_4.186381"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="PLZ75_08485"
     CDS             <1..1418
                     /locus_tag="PLZ75_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS72468.1"
                     /translation="YFLRAYRYYCKTLGYGDVPLVLEELSAPKLDFYSTTKESIWKKM
                     IKDLEFAIDHVPEANMVPRGQVTKAACKHLLAKYYLLFGKFDEAITLTTEIIEGGVHQ
                     LNTERFGVDKNIPNKDVVWDMFRVENKSLASNTEGLLMSIDRYGMDGNTSGTNSMRNA
                     GPNFGQAGIIKTPDGANGMSDTPGCEYGLIEMYGRGVARIRPTGYSQKDVWILNGIED
                     FQDYRHRTDNGNWVTMEMMVYNNPALKTANNVWYGKNLMLFNESGVLLCNDIIRSWFA
                     WPHYKLFVPDPDRPQPQGGPSDWYIMRLAETYLIRAEAHVWKKEWQKAAEDINVIRQR
                     ANAQYMYTADDLEELQIGAVLDERNRELYYEELRKFELTRISYIFASTNIQCYNGKTY
                     TLDKLSEDNFYYDRIMEVGDHYNKGIITRTGNPFTISPKHIFWPVASYVINSNTGGVI
                     NQNKGYYGTEKNVPPLVYEGD"
     gene            1566..2807
                     /locus_tag="PLZ75_08490"
     CDS             1566..2807
                     /locus_tag="PLZ75_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72469.1"
                     /translation="MIKIIHRKLFSLLFFTLFIGFVDGQSLPGSYFSPWSVGLLDIHH
                     INTGKGESVFIIMPDGTTMLVDAGATTRPKPRVTDQKPDASRTPGEWISRYILHMMKD
                     IPSKKLDYILLTHFDGDHIGDYSDKAKTSSSGTFKLTGITEVGEHVPFKKLVDRGWPN
                     YNFPIALSNDKMRNYKQFLKWHIEKNQSIIEDFKVGQNSQFVLVYAREEYPDFEIRNI
                     ACNGYVWTGVGTNVRNHFPKLSDLKISEYPGENMCSIAFRLSYGKFDYFNGGDLTFGE
                     YGTWRDIETSVGMVTGPVEVCESNHHGHFDAMGEHFLKALRPKIIILQTWAPSHPASV
                     VLSRMQSTSIYPGPRNIFATNIMKETKVVIGDALDRLKSQQGHIVVRVNPGGDDFMIY
                     ILDDLTESYEVKAVHGPYYCE"
     gene            3123..3389
                     /locus_tag="PLZ75_08495"
     CDS             3123..3389
                     /locus_tag="PLZ75_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOS72470.1"
                     /translation="MKRSRFTESQITSALKEHEAGKNVLDICRELRINRNTFYNWKKR
                     YSGMDAELLRRFKELERENAELKRMYADLSLDHRILKNVIEKKL"
     gene            3413..4228
                     /locus_tag="PLZ75_08500"
     CDS             3413..4228
                     /locus_tag="PLZ75_08500"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOS72471.1"
                     /translation="MREIREEEQISVARACKIVMLVRCMYYYESKKDDSEVEAKLQWY
                     GEKLPSRGFPEYFKRIRGEGLIWNHKKVRRVYLKLGMARRRKIKRRIPNPDRQVLLQP
                     VRPNITWSLDFMEDRLENGRRFRTLNIIDDYNREALAIEVDYSFPSAKVVDMLKRVIE
                     WRGKPEEIRSDNGIEFIAKDFSVFCSNSGINHLRIQKGKPSQNGYIERFNRTYREDVL
                     DINLFESIQQVRIKTEEFMEDYNNYHPHESLNNMSPVKFLKSKFKEKMPTFTL"
     gene            4430..6610
                     /locus_tag="PLZ75_08505"
     CDS             4430..6610
                     /locus_tag="PLZ75_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72472.1"
                     /translation="MVHKESKQLFIGPYAIDADRNVRTIPYEKMPGRHTGNARHLTDP
                     AGKIYYATMEEGFYEVDVNTLEIKLLYQDGNVTDRKQDVVSANKPAALLEGVHGKGFY
                     SGQGVAVYTNNGESGNLALVKFDIEAGSLSEWNGKEWKLIRRNQFCEITGPGGIYGNE
                     NPETDPLWATGWDHKSLILGVRNGGNWSFYRLPKASHSYDGAHGWNTEWPRIRNIGTD
                     GRPDYLMTMHGMFWNFPGNFTASNSSGIRPRSAYLKIIGDFTRWNNRLVFGCDDSALN
                     EFLNKRKEKGSIEDPGQSNSNLWFTSLKQPDELGPTTAEGAVWLNEKTEAGMPSEPFL
                     FAGWARRISWIHNSGDSRVNFIFEVDKSGNDLWETLQSVAIDGNMSALIEFPAATRAE
                     WIRVKTDKPAIATVHFSYTGKDDRNDCPDPVFNGLSVVTDQLSTGGLLYGLGNDRRAL
                     GVLAGQFSGGDFTETGYYELNADMDLQKKEDPETAGFIRDKFAIPQQVVTIDEASVLI
                     QDDFGRRWRLPVGNKAYTELTERSALRLCREVVTERDLFSCAGTFYELPANNADGFAK
                     IRPVASHNFRIHDYASYRGMLIMTGIDPGKARGNSHVIVSDDGKAAVWAGVIDDLWKI
                     GKPAGEGGPWKNSHVSAGIPSDPYLIGFYDKKSLELSHNSPHRITFTVEAEPLGHGPW
                     MTYKKIEVGPGETAGFNFPDNFQSRWIRFTSDRDCIATVWLKYE"
     gene            7005..>7427
                     /locus_tag="PLZ75_08510"
     CDS             7005..>7427
                     /locus_tag="PLZ75_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72473.1"
                     /translation="MKRREVLKTLSTIPVAGGLMSIEPLVASASQRKPVIDAGLLSNP
                     EKGALALGPQIYQSIGVEPLINCRGTLSIISGSTELYEVRKAVEYAAMVHVHIDELAM
                     AVGKRLAEITGAEWGMVSSGCAAGLKHVTAACLTGGDPE"
BASE COUNT         2353 a         1283 c         1743 g         2048 t
ORIGIN      
        1 cctacttttt acgtgcttat agatattatt gtaaaacatt aggatatggg gatgtgccgc
       61 ttgttcttga agaactttcg gccccaaaat tagacttcta ttcaactaca aaagaatcta
      121 tttggaaaaa aatgattaaa gatttggaat ttgcaattga tcatgtccct gaagcaaata
      181 tggttccaag aggtcaggtt accaaagcag cctgtaagca tcttttggct aagtattact
      241 tgttattcgg aaaatttgat gaagctatta ctttaactac cgaaattatt gaaggaggag
      301 ttcatcaatt aaatacagaa agatttggag tagataaaaa tattccgaat aaagatgtgg
      361 tatgggatat gttcagagta gaaaacaaga gccttgctag taataccgaa ggattactca
      421 tgtctattga ccgttatggg atggatggaa atacttctgg tacaaattct atgagaaacg
      481 caggtcctaa ctttgggcaa gctggaataa tcaagacacc cgatggtgca aatggtatgt
      541 ctgatacacc cggttgtgaa tatgggctta tagaaatgta tggcagaggt gtagcccgta
      601 ttcgcccaac aggttattca caaaaagatg tctggatcct taatggcata gaagattttc
      661 aggattatcg tcatagaact gacaatggaa attgggttac catggaaatg atggtttata
      721 ataatcctgc acttaaaacg gcaaataatg tatggtacgg taaaaatctc atgttattta
      781 acgaaagcgg tgtattgctg tgtaatgata ttattagatc atggtttgct tggccacatt
      841 acaaactatt tgtacctgat cctgaccgcc ctcaacctca aggtggaccg agtgactggt
      901 atattatgcg tctagcagaa acgtatctta ttagggccga agctcacgtt tggaaaaaag
      961 aatggcaaaa agcagcagag gacataaatg taattcgaca acgtgcaaat gctcaatata
     1021 tgtacaccgc agatgatctt gaggaactgc aaattggtgc tgtacttgat gagagaaata
     1081 gagaattgta ttatgaagag ctacgtaaat ttgagcttac acgtatttct tacatatttg
     1141 caagtacaaa tattcagtgt tataatggga agacatatac acttgataag ttatcagaag
     1201 ataatttcta ttatgataga ataatggaag ttggagatca ttacaataaa ggaattatta
     1261 caaggacagg taatccgttt acaatatcac caaaacatat tttttggcct gttgccagtt
     1321 atgttatcaa ttcaaataca ggtggtgtta ttaatcaaaa taaaggctat tatggaacag
     1381 agaaaaatgt tccacctttg gtttatgaag gagattaatt ttggaaatat taataaactg
     1441 cacattcgaa ttccttagaa tgtgcagttt atgtttttaa agattctttc ttgaaaaggc
     1501 tatgaaaact aagtgtcaga tattgaggag attactataa acgaattagg tcaattaaaa
     1561 tgaatatgat aaaaattatt cacaggaagt tattttccct actatttttc acattgttca
     1621 ttggctttgt tgatggacaa tcgttaccag gaagttactt ttcaccttgg tcagttggcc
     1681 tgctagatat ccatcatatc aatactggca aaggggagtc tgtctttatt attatgccag
     1741 atggaactac aatgttggta gatgcaggag caacaacacg tcccaaacca cgcgtcacag
     1801 accagaaacc agatgcaagc aggactccgg gtgaatggat atcaaggtat attttgcata
     1861 tgatgaaaga tattccatca aaaaaactag actatatact tcttacacat tttgatggag
     1921 atcatatcgg ggattattcg gacaaggcaa aaacatcaag ctcaggtaca tttaaactta
     1981 caggtataac ggaggttgga gaacatgtac cttttaaaaa gttagttgat agaggctggc
     2041 ccaattataa tttcccaata gctttgagta atgacaaaat gaggaattac aaacaatttc
     2101 taaagtggca tattgaaaaa aaccaatcta tcattgaaga ttttaaagtt ggtcaaaatt
     2161 cacaatttgt tttggtttat gcgagagaag aatacccgga ttttgagatt cgaaatattg
     2221 catgcaatgg atatgtttgg acaggagtag gaacaaatgt tcgaaatcat ttcccgaaac
     2281 tttcggattt aaaaatatca gaatatcccg gagaaaatat gtgctctatc gcgtttcgat
     2341 tgtcttatgg aaagtttgac tatttcaatg gtggggatct tacttttgga gaatatggta
     2401 cttggagaga tattgaaaca tctgttggga tggtcacagg acccgtcgag gtttgtgaat
     2461 caaatcatca tggacatttt gatgcaatgg gagaacattt tctaaaggct ctcagaccaa
     2521 agattattat acttcaaact tgggcgccta gtcatcctgc ttcagtagtg ttaagccgaa
     2581 tgcagtcgac ttcaatttat ccaggtccga gaaatatttt tgctaccaat ataatgaaag
     2641 aaacaaaagt tgtaattggt gacgctcttg atagactaaa aagccagcag ggacatatcg
     2701 ttgtaagagt gaaccctggt ggggatgatt tcatgattta cattcttgat gatttaacag
     2761 aaagctatga ggttaaggca gttcatgggc cgtattattg tgagtaacag ttcttaaaga
     2821 aaaactatga aaatatcttg gctttaaaaa taaattagta attcaaagca tcatgggata
     2881 attaatacac attgaggcat ttagttaatt atatataaaa taattagaaa aaaaaatatc
     2941 attcagtaca aatacttgtt tttgttctgg ttgtatgttt aatttcaact ggttgctcca
     3001 gaaggaatga tggtgacaaa tacaagtctt tcagcggtat ttatcctcat ctggcattga
     3061 agatatccct ataaatccta caagttataa ttgggcaaaa atgtctaatt ttaaacttgt
     3121 atatgaaaag atcaagattt accgagagcc agataacaag cgcgctgaag gagcacgaag
     3181 ctggcaagaa tgttctggac atatgtcggg aactaagaat taatcgcaac accttttaca
     3241 attggaagaa gcggtattcg gggatggatg ccgaacttct tcgaaggttc aaggaacttg
     3301 agcgggagaa tgcagagtta aagcgcatgt atgccgacct gagtctggat cacaggatat
     3361 taaagaatgt catagaaaaa aagctttaac gccctgtcaa aagagagagt tggtgagaga
     3421 gataagagaa gaagagcaga tatctgttgc cagggcgtgt aagattgtaa tgctggttcg
     3481 ctgcatgtat tactatgagt ctaaaaaaga tgattcagaa gttgaggcaa agctgcagtg
     3541 gtatggagag aagcttccct caaggggctt tcctgagtat tttaagagga tccgtggaga
     3601 ggggcttatc tggaaccaca aaaaagtccg aagagtatac ctgaagcttg gaatggctcg
     3661 tcgaagaaag ataaaacgca ggataccaaa tcctgatagg caagtcctgt tgcagcctgt
     3721 cagacccaat atcacctgga gtcttgattt tatggaggac cgactggaga acggaaggcg
     3781 gtttagaaca ttgaacatta ttgatgatta taacagggaa gcacttgcaa tagaagtcga
     3841 ttactcgttc ccttcagcaa aagtagttga tatgttaaaa cgagttattg aatggcgggg
     3901 taagcctgag gagatccgca gtgataatgg gattgagttt atcgcaaaag attttagtgt
     3961 cttttgctca aactcaggga tcaatcatct tcgaatacag aaaggcaagc ccagccagaa
     4021 tgggtacatt gaaaggttta acagaacata tagagaagat gttcttgaca tcaatttatt
     4081 tgaaagcatt cagcaagtca ggataaagac tgaggaattt atggaagatt acaataacta
     4141 tcatccacac gaaagtctca acaatatgag cccggttaaa tttctaaagt caaaattcaa
     4201 agaaaaaatg cctactttta ctttgtagga gaaagggggt atctacagca tttacaataa
     4261 tgaaagtgaa tgtggtacag gagctgttgt tccctgggct gacaggttgt gggtgatcac
     4321 ttacgggccg catctgcctt ttggttcttc tgacaaatta tatgagataa ctcctgatct
     4381 tcagcagata atcagaaagg agagtgtcgg tggaactcct gcaaaccgta tggtacataa
     4441 agagagtaag cagcttttta taggacccta tgccattgat gcagatcgta acgtaaggac
     4501 gattccctat gaaaagatgc cggggagaca tacgggaaat gcgcggcatc tgacagatcc
     4561 tgccggaaag atttattatg ccacaatgga ggagggattt tatgaagtgg atgtaaatac
     4621 actggaaata aaactgcttt atcaggacgg aaatgttact gacaggaaac aggatgtggt
     4681 gtcagcaaat aagccggctg cacttctgga gggtgtacac ggcaaaggtt tctattcagg
     4741 gcagggtgtt gctgtttata ccaacaatgg tgaatcggga aacctggctc ttgttaagtt
     4801 tgatattgaa gcaggttccc tgtcagaatg gaatgggaaa gaatggaaac tgatccgccg
     4861 taatcagttc tgtgaaatta ccggtcccgg tggaatatac ggcaatgaaa accctgaaac
     4921 cgatcccctg tgggccaccg gctgggatca taaatccttg atacttggtg tgagaaacgg
     4981 aggaaactgg agcttctacc gacttcccaa agcgagtcac agctatgatg gagctcacgg
     5041 ctggaatacg gaatggccgc gtatccggaa tatcgggacg gatggcaggc cggattacct
     5101 gatgaccatg cacggtatgt tctggaactt tccgggtaat tttaccgcat caaattcatc
     5161 cgggatccgt ccccgatcag catatctgaa aataatcggg gattttacca ggtggaataa
     5221 cagactggtt tttggttgtg acgattcagc actgaatgaa ttccttaaca agcgcaaaga
     5281 gaaaggcagt atagaagatc caggccagtc gaactccaat ctgtggttta cttcgttgaa
     5341 gcagcctgat gagcttggtc ccacaacggc tgaaggggcg gtgtggctca atgaaaagac
     5401 tgaggcagga atgccttcag aaccctttct ttttgccgga tgggccagac gtatttcctg
     5461 gatacataat tcaggcgatt ccagggtgaa tttcattttt gaagttgaca aatcaggcaa
     5521 cgatttgtgg gaaacactac aatcggttgc aattgacgga aatatgtcag ctttgattga
     5581 gtttcctgca gccaccaggg ccgagtggat aagggtaaaa accgataagc cggcgattgc
     5641 aacggttcat ttttcatata ccggcaagga tgacagaaat gattgccctg atcctgtttt
     5701 taacgggtta tcggttgtca cagaccagct gtcaacaggc ggcctgctgt atggtcttgg
     5761 gaatgaccgc cgggcattgg gagtgctggc aggacaattt agcgggggag attttacgga
     5821 gactggttat tatgagctga acgctgatat ggatcttcag aaaaaagaag atccggaaac
     5881 agccgggttt atacgagata aatttgcaat tcctcaacaa gtggtaacga ttgacgaggc
     5941 ttctgtgctt atacaggatg attttggcag gagatggagg ctgccggtgg gcaataaggc
     6001 ttatacagaa ctgacagagc gttcggcact gcgtctttgc cgcgaagtgg taactgagcg
     6061 ggatcttttc agctgtgcag gaacatttta tgaacttcct gccaataatg ccgatggatt
     6121 tgcaaagatc cgccctgttg catcacacaa cttcagaata catgattatg cttcctatcg
     6181 ggggatgctg atcatgacag gaattgatcc gggcaaagcc cgcggcaact cacatgtaat
     6241 tgtgtctgat gacggaaaag ctgctgtatg ggctggtgtg atcgatgatc tttggaaaat
     6301 aggcaaacct gccggagagg gtggtccgtg gaaaaacagc catgtttctg ccggcatacc
     6361 atccgaccct tacctcatag gtttttatga taagaaaagt cttgaactgt cacataattc
     6421 tccacacagg attacattta cagtcgaggc agaaccgctt ggtcatgggc catggatgac
     6481 ttacaaaaaa attgaagtag ggccggggga gacggccggt tttaatttcc ccgacaactt
     6541 tcagtcgcgc tggataagat tcacttcaga cagggattgc attgcaacgg tatggctgaa
     6601 atatgaatga atggatagca aaggccggac taccggaact atatgcataa aaagatgtga
     6661 caggtgaatc aaatatgaga ggacctttct tgacatccgg aaaacagggg gtgaattatt
     6721 tcctgccgga gtgaagttaa ttcaatattt tcaggaaaag ccgtgatcat tctttcgccg
     6781 ggaatagccc gagaattcct gtatgactta tgtctgaatg aactattaat aatttttaag
     6841 atttgaattt cccctaagcc cttttttccc cgcataaaca tttaactttg ataaaaataa
     6901 tgccaatcag cttacaccat ctcataagta tatgctgtat tgatacgcag tcaggaaaac
     6961 tgccacattt aaaaacttat taatctgctt taattaaatt aaatatgaaa cgacgcgaag
     7021 tattaaaaac tctgtcaaca attccggttg caggaggatt aatgtcaatt gaacctctgg
     7081 tagcatcagc aagccagagg aagcctgtta ttgatgcagg gctgttgtca aatccggaga
     7141 aaggggcact ggctctcgga ccacaaattt accagtcaat aggcgtggaa cccttgatca
     7201 actgcagggg gactctttcg ataataagtg gttcaacaga actgtatgaa gtcaggaaag
     7261 cagttgaata tgctgcaatg gttcatgtac atattgatga actcgcaatg gcagtgggta
     7321 aaagactggc tgagatcaca ggagctgaat ggggaatggt atcatcaggc tgtgccgcgg
     7381 gtttaaaaca tgttactgcc gcctgtctaa ccggtggcga tcctgag
//
LOCUS       DAOSPX010000155         7407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9335_length_7407_cov_4.791349, whole genome shotgun sequence.
ACCESSION   DAOSPX010000155 DAOSPX010000000
VERSION     DAOSPX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9335_length_7407_cov_4.791349"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLZ75_08515"
     CDS             complement(<1..713)
                     /locus_tag="PLZ75_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72474.1"
                     /translation="MKTIAGAAIGSCVHVGGLHHFLKLAETEGYHTVSYGPAVSVERI
                     VKIIREVNPDIMAISYRLTPESAAELFSDLKMQLERAGVSNIRMLFGGTPPVAQKARE
                     SGLFEYAFDGTESADLIKRYLRGDSGSGTGESLPDNLVDRISRKYPYPLLRHHFGRPT
                     LQETVEGAARIAESGVLDILSIGTDQNAQEFFFRPEKMDHSQDGAGGVPVRKPEDLAA
                     IYESTRRGNFPLVRCYSGTR"
     gene            complement(780..1889)
                     /locus_tag="PLZ75_08520"
     CDS             complement(780..1889)
                     /locus_tag="PLZ75_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOS72475.1"
                     /translation="MTNKTTRNKLRIAVLGAGHGGLAMAGHLALMGHRVNLYNRGEER
                     LWGVKSSGTIDITGEVEGSGVIDLATTCMKEAVSGVDLIMVAVPANGHRWMAEEMAPH
                     LTDNQIIILHPGRTFGALEFKQVLNRLRVKADVIIAEAQTFIYASRATGPSQVHIFRI
                     KHSIPVASIRAHLIPEVISKLRQFYPQFVPGDNVVKTSFENIGSVFHPAICILNAGWI
                     EHDSDFQFYHEGATRGVTRILEKIDEERVKVAEALGIRVISARQWLYMAYSSAGNTLF
                     EAMQKNVGYRGIMAPRSLKIRYIEEDVPFSLVPIASTGKMLNVPTPTIDSVIQLSCAL
                     NDFDYWSNGRTVESLGIAGMSVRDLRLLAIGETAR"
     gene            complement(2020..4629)
                     /gene="mutS"
                     /locus_tag="PLZ75_08525"
     CDS             complement(2020..4629)
                     /gene="mutS"
                     /locus_tag="PLZ75_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOS72476.1"
                     /translation="MPESLKYVETPLMKQYNSIKGKHPDAILLFRVGDFYETFGKDAI
                     RASEILGITLTRRANGAASFVELAGFPHHALDTYLPRLVRAGQRVAICEQLEDPKLAK
                     KIVKRGVTELITPGVSMNENVLNHKENNFLAAVHIDKKIAGLAFLDISTGEFFVAEGT
                     FEYADQLLNKFQPREVLYESRKKELFMENFAEKFYTYRLDDWIFNSESAAERLVRQFE
                     TNTLKGFGIQNMPYGIVAAGAVLYYLDMTHHEQLGHIRSISRIDEDDYVWLDKFTIRN
                     LELFNSPYEGAKTLIDVVDRTISPMGGRLIRRWLSLPLKDINTVNERLDIVEYLVEQD
                     AFREQLSDLITQTGDLERLLSKVAVGRINPREVVQLKRALQAIDTLKSLCCDSTCPPL
                     VRRGRELDPCRALAERIGHDLNDDPPVQISRGNIIARGISAELDELRNILSSGKDYIK
                     DLQEREIRRTGISSLKVSYNNVFGYYIEVRNTHRDKVPSGWIRKQTLVSAERYITEEL
                     KEYEEKILGAEDKIIDIETRIFNDLVLEILNYIQPVQHDAAIVAGLDCLQSFAVISGE
                     NVYVRPQLDDSGIIEIHDGRHPVIEKQLPAGEAYIPNDLNLDREDQQIIILTGPNMSG
                     KSALLRQTALIVLMAQTGCFVPARAARIGLVDKIFTRVGASDNISLGESTFMVEMNET
                     ASILNNLSDRSLVLLDEIGRGTSTYDGISIAWAMVEYLHENRMRAKTLFATHYHELNE
                     MENSFPRVRNYNVSIRELNKKIIFLRKLKRGGSEHSFGIHVAKMAGMPRSIVMRADEI
                     LKELERSHQKQELTKPIADLAGHREGLQLSIFQLDDPVLKQIRDEILEIDIDNLTPVE
                     ALNKLYNIRKYLK"
     gene            complement(4780..5490)
                     /locus_tag="PLZ75_08530"
     CDS             complement(4780..5490)
                     /locus_tag="PLZ75_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOS72477.1"
                     /translation="MASDFFSFKQFRIRQDRSIFKVGTDAVLLGAYADVNSARTILDI
                     GTGTGLIAIMLAQRCEAEITAIEPDPCSFEQALSNVRQCSWSSRISVVNTELQNYYPG
                     NEKFDLIVCNPPYFVDSLKNPDPRKASARHNVSLNRNDLLSGISRLLADNGRFQLILP
                     LAEGNIFIEEAGYSGFYCYDILKIIPVPGAGIRRMILSFSRNRQETAENILTIEGGGQ
                     KGFTEEYKNLTKDFYLKF"
     gene            complement(5515..>7407)
                     /locus_tag="PLZ75_08535"
     CDS             complement(5515..>7407)
                     /locus_tag="PLZ75_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72478.1"
                     /translation="PGVASFSNPAWSPGGDEIAVSGLVEGIGDLYLFNIYTGEVRRLT
                     GDNEANLLPSWSPDGNYIVFSQEKANPPGGKRKYSYNLSIYDLKKGSSTEIDIFQGAY
                     NMNPHFSADGRSVYFLSDADGFRNLYRYDTETKRVFRLTHYITGISGITPYSPALSVS
                     LKNDLIVYNYYFNNRYQIIAADKTDFTEDERDGNYLNFEAGTLPPYDHVSANIVDRNL
                     YDKRIVADISPDQAVKIPYKSKFRLDYISNSANIGLSTGMYRNNMSGSVNMLFSDMVG
                     NNQMFATLALNGEIYDFGGQTAFINQTGKIKWGASLSHIPYLYGDMLFSRDTIKINDE
                     LVPVLNMGVDYIRMFEDNISLFASYPLSQTRRFEANASSSWYYYRIDRFTYYYLMNGY
                     YVGGKREKLDAPKGGNYQQASIAFVSDNSYFGMTAPMQGSRSRYQLEKYFGSLNIYTI
                     LIDYRQYFKFRPLSLAFRIYNYSMHGKDSEDGIIPPFYLGYPWLIRGYENISYSGNNL
                     MRGETFNMSRLSGTRILVANAEVRIPFTGPERLALIKSKWLLTDLNLFFDSGLAWNRG
                     DKLGFVKDLTGGAANDYRFPLFSTGASVRINLFGYLVLEPYYAFPLQNGGFRNGTFGL
                     NFVPGW"
BASE COUNT         1856 a         1889 c         1595 g         2067 t
ORIGIN      
        1 cgggtgccgg aatagcatct gacaagcgga aaattccccc ttcttgtgct ttcatatatt
       61 gcagccagat cttcaggttt tcttaccgga acacctccgg ccccgtcctg gctgtgatcc
      121 attttttccg gccggaaaaa aaactcctgg gcattctgat ccgttcctat ggagagtata
      181 tcgagaactc ccgattcagc aatccgggca gccccctcaa cggtctcctg aagtgtgggc
      241 ctgccaaaat gatgccggag caaaggatag ggatatttcc tgctgatcct gtcaaccagg
      301 ttgtccggca ggctttctcc cgtaccgctt cccgaatcac ctctcagata acgctttatc
      361 agatcagcag attcggttcc gtcaaaagca tattcaaaaa gaccggactc acgtgctttc
      421 tgtgccaccg gaggtgttcc cccgaacaac attctgatat ttgaaacccc ggctctctca
      481 agctgcattt tcagatcaga aaaaagttct gcagccgatt caggtgttaa cctgtaactt
      541 attgccataa tatcgggatt aacctccctg ataattttta caatcctttc aactgacact
      601 gcaggaccat aggaaactgt atggtagcct tccgtttcag caagtttgag gaaatgatga
      661 agtccgccaa cgtgaacaca actgccaatg gcagcacctg caatagtttt catttcttta
      721 tggtttaaaa ttattcctga atatttccgg ttcagcaatg attcccggaa aatatataat
      781 tacctggcgg tctcgccaat ggccagaagc cttaaatccc gaacactcat tccggcaatg
      841 ccaagacttt ccactgtccg cccgtttgac cagtaatcaa aatcattaag ggcacagctc
      901 agctgaatca ctgaatctat ggtcggtgtg ggaacattca gcattttccc tgttgaagct
      961 atgggaacca ggctgaaagg gacatcctct tctatatatc ttattttcaa ggatctggga
     1021 gccataatac ctctgtagcc tacatttttc tgcatggctt caaaaagggt atttcctgca
     1081 gaactataag ccatatacaa ccattgcctt gcggaaatca cccttattcc aagagcttcg
     1141 gcaaccttta ccctttcctc gtcaattttc tcgagtatcc tggtaactcc ccgggttgca
     1201 ccttcatgat aaaactgaaa atcactgtca tgctctatcc agccggcatt gagaatacat
     1261 attgcaggat gaaaaacact tccgatattt tcaaagcttg tctttacaac attgtcgccc
     1321 gggacgaact ggggataaaa ctgacggagt ttacttatca cctcgggaat gagatgcgcc
     1381 ctgattgaag ccacgggaat ggaatgcttg attctgaaaa tgtgaacctg agacggcccg
     1441 gtggcccgcg atgcatatat gaaggtctgc gcttcggcaa ttatcacatc ggccttaacc
     1501 ctgagccggt tcagaacctg cttgaattca agtgccccga aagtccttcc gggatgaaga
     1561 atgataatct ggttgtctgt gagatgagga gccatttcct cagccatcca cctgtgcccg
     1621 ttggcaggca cagcaaccat tataagatca acccctgaaa ctgcctcttt catgcaggtg
     1681 gtcgcaaggt cgataacccc ggagccttca acttcacctg tgatatctat tgtgcctgac
     1741 gatttaactc cccacagcct ttcttccccc ctgttgtaca ggttcaccct gtggcccatg
     1801 agagccaggt gccctgccat ggccagtccg ccatgccccg cccccagaac tgctatcctg
     1861 agtttattcc tggtagtttt atttgtcata agcatggatt taaatgcaca tattaatata
     1921 cgaatttttt tatgatttat ttcaataccg gattaatcca ttcttttaca ggtataattc
     1981 ctgaatacat attgccggcg atcatcccgc cctgccagat catttcaggt atttcctgat
     2041 gttatacagc ttattcaatg cctcaaccgg ggtcagattg tcgatatcga tctccagtat
     2101 ctcatcacgg atctgtttga gcaccggatc atccagctgg aaaatactga gctgcaatcc
     2161 ctccctgtga ccggcaaggt ctgcgatagg cttggtcagc tcctgtttct gatgactccg
     2221 ttccagttcc ttcagtattt catccgcacg cattacgatg cttcgcggca ttcctgccat
     2281 ttttgcaaca tgaatgccaa aactgtgctc actccccccc cttttcagct tgcgcaggaa
     2341 aattatcttc ttgttcagtt ccctgatcga tacattgtaa ttcctgactc ttggaaatga
     2401 gttttccatt tcattgagtt catgatagtg ggtggcaaac agcgtttttg ccctcattct
     2461 gttctcatga agatattcaa ccatcgccca tgcgattgaa ataccgtcat aggtgctggt
     2521 acctcgcccg atctcatcaa ggaggacaag tgacctgtcg gacaaattat tgaggatgct
     2581 cgcggtttcg ttcatttcca ccatgaaggt ggattccccg agactgatgt tgtccgatgc
     2641 ccctaccctg gtgaatattt tatcaaccag gcctatccgt gccgcacggg ccggaacaaa
     2701 acagcctgtc tgggccatga gcactataag ggcagtctgc cggagcaggg cggatttgcc
     2761 cgacatgttc ggaccggtca gtattatgat ctgctgatct tccctgtcaa ggttaagatc
     2821 attgggtata tatgcctcac ctgcaggaag ctgcttttca atgacaggat gccgtccgtc
     2881 atgaatttct atgatgcccg aatcgtcaag ctgaggcctg acatagacgt tctcccctga
     2941 aatgacagca aatgactgaa ggcagtcaag cccggcaacg atggccgcat catgctgaac
     3001 aggctggata taattcagta tttccagaac cagatcattg aagatccttg tttcaatatc
     3061 gattatctta tcttcagctc ccagtatttt ttcctcatat tccttcagct cctcagttat
     3121 ataacgttcg gcactgacga gtgtctgctt ccttatccat ccggaaggca ccttgtcccg
     3181 gtgagtgttt cttacttcaa tataatagcc gaaaacatta ttgtagctca ccttgagtga
     3241 acttatgccg gtacgccgga tctccctttc ctgaagatct ttgatataat cctttccgct
     3301 ggagagaatg ttccgcagtt catcaagctc agccgatatt ccccgggcaa ttatattccc
     3361 cctgcttatc tgtaccgggg gatcatcatt gagatcatgt cctatccgtt ccgccagtgc
     3421 cctgcacggg tcaagctccc tgccccggcg gacaagaggc ggacaggtac tgtcgcaaca
     3481 cagtgactta agcgtgtcaa ttgcctgcag ggcccttttc agctgaacaa cctcccttgg
     3541 gtttattctg ccgacagcaa cctttgaaag aagcctttca agatctcctg tctgtgttat
     3601 cagatccgac agctgctccc ggaacgcatc ctgctccacg agatactcaa caatatcaag
     3661 tctctcattc acggtattga tgtccttcag gggaagagac agccatctcc ttatcagcct
     3721 cccgcccatg ggacttattg ttctgtccac tacatcaata agagttttcg cgccctcata
     3781 gggggaattg aacagctcga ggttcctgat ggtgaattta tcaagccaca cgtaatcgtc
     3841 ctcgtctatg cgggaaatgc tcctgatatg gcccagctgc tcatgatggg tcatatccag
     3901 ataatacagc acggctccgg ctgcaactat tccgtatggc atgttctgaa tgccgaaacc
     3961 cttgagtgtg tttgtctcaa actgcctgac cagccgttcg gctgccgatt cagaattaaa
     4021 gatccagtcg tcaagcctgt aggtataaaa tttctcagcg aaattctcca tgaaaagctc
     4081 ctttttcctg ctttcataga ggacttccct tggctgaaac ttattcagta actggtcggc
     4141 atattcaaat gtaccttccg caacaaagaa ttcaccggta gaaatatcca gaaatgcaag
     4201 cccggcgatt tttttgtcta tatggacagc ggcaagaaaa ttgttttcct tgtggttcag
     4261 cacattctca ttcatggaaa ctcccggggt aatcagttcc gtcacacccc tcttcactat
     4321 tttctttgca agtttcggat cttcaagctg ctcgcatatt gcaaccctct gaccggccct
     4381 gaccagtcgc ggcagatagg tgtcaagcgc atgatgcgga aaacctgcaa gttcaacaaa
     4441 tgaagccgcc ccgttggccc tgcgagtaag cgttattccc agtatctccg atgccctgat
     4501 ggcatccttg ccgaatgtct cataaaaatc accgacacgg aaaagaagta tggcatcagg
     4561 gtgtttgccc ttgatgctgt tatactgctt catcagagga gtttcaacat atttcagact
     4621 ttccggcatc agttttgttt aaatgatatt tctgcctttc cgtgacccgg acaaagatac
     4681 agcgacagca ttactattga aaatttttgc agacatttat tatccagcct ctttaacagc
     4741 cggatagccc ggccggcagt taacttcccg cccggaaggc taaaatttca gataaaagtc
     4801 ttttgtaaga tttttgtatt cttccgtaaa acctttctgc ccgcctcctt caatggtaag
     4861 gatgttttct gctgtttcct gcctgttacg cgaaaagctc agaatcattc tcctgattcc
     4921 ggcccctggc acaggtataa ttttcaggat atcataacag taaaatcccg aatatccggc
     4981 ttcttcaatg aaaatattcc cttcagccag gggcaggatt aactgaaacc ggccattgtc
     5041 cgcgagcagg cgcgatatac cgctgagcag atcattcctg ttcaggctta cattatgtct
     5101 tgccgatgct ttacggggat cgggattctt caatgaatca acgaaatatg gaggattgca
     5161 tacaatcagg tcaaactttt cattgccggg ataataattc tgcagttccg tattgacaac
     5221 gcttatcctt gacgaccagc tgcactgccg gacattgctg agagcctgtt caaaggaaca
     5281 gggatccggt tcaatggccg ttatctcagc ctcacatctt tgtgcaagca ttattgcaat
     5341 aagacccgtg ccggttccga tgtcaagaat tgttcttgcc gaatttacat cagcataggc
     5401 gcccagcagc acggcatcgg ttcctacctt gaaaatgctc ctgtcctgcc ttatcctgaa
     5461 ctgtttgaat gaaaagaaat ctgatgccat cttgtgttga aaggatccgg taaactacca
     5521 tccgggcaca aagttcagac caaatgttcc gttccggaat cccccgttct gtaacgggaa
     5581 ggcataataa ggctcgagga caagatatcc gaaaaggttt atcctgacgg aagcaccggt
     5641 gctgaaaagc ggaaacctgt agtcattggc agctcctccg gtcagatcct tcacaaaacc
     5701 cagtttgtcg ccccggttcc atgccagtcc tgaatcaaaa aaaagattca gatcagtcag
     5761 cagccacttt gatttgatca gggccagtct ttccggaccc gtgaagggaa tcctgacttc
     5821 cgcattggcc accaggatcc tggttccgct cagtctggac atattgaagg tttcgcccct
     5881 catgagatta ttacccgaat aggaaatatt ttcatagccc cttataagcc agggatagcc
     5941 aaggtaaaag ggaggaataa tcccgtcttc cgaatccttg ccgtgcatgc tgtaattata
     6001 gatccggaat gccaggctca ggggcctgaa tttgaaatac tgcctgtaat caatcagaat
     6061 ggtatagata ttcagtgacc cgaaatattt ctcgagctgg tatcttgatc tgctgccctg
     6121 catgggtgca gtcatgccaa aatatgaatt gtcactgaca aaggcaattg atgcttgctg
     6181 gtaattaccg cccttcggag catcaagctt ttcccttttc ccgccgacat agtaaccatt
     6241 catcagataa tagtatgtaa acctgtcaat gcggtaatag taccatgatg aggatgcgtt
     6301 ggcctcaaat ctccttgtct gtgacagcgg atatgatgca aacagtgaga tattgtcctc
     6361 gaacattctt atataatcaa ctcccatatt taaaaccggg accagctcgt catttatttt
     6421 tatggtatca cgtgaaaaca acatgtcccc atacagatag ggtatgtggg aaagggaagc
     6481 tccccatttg atttttccgg tctggtttat gaaagctgtc tgtcccccga aatcatatat
     6541 ttctccgttc agggccagtg tggcaaacat ctggttgttc ccgaccatgt cgctgaataa
     6601 catgttaaca cttccgctca tgttgttcct gtacatcccg gtggagaggc cgatatttgc
     6661 actgtttgag atatagtcga gcctgaattt tgatttgtag ggtattttca cggcctgatc
     6721 cggggatata tctgccacaa tacgcttgtc ataaagattc cggtcaacaa tatttgccga
     6781 aacgtggtcg tagggaggaa gtgttcccgc ttcgaaattc aggtaattcc cgtcccgttc
     6841 atcctcggtg aaatcagttt tatccgctgc aattatctga tacctgttgt tgaaataata
     6901 attgtagaca ataaggtcat ttttcaggga aacactgagt gcaggggaat agggggttat
     6961 gccgctgatg ccggtaatat aatgcgtaag acggaaaaca cgtttagttt cggtatcata
     7021 cctgtaaaga ttccggaatc cgtcagcatc cgaaagaaaa taaaccgacc tgccgtcagc
     7081 ggaaaaatgc gggttcatat tgtatgcccc ctgaaagatg tctatttctg ttgaagaccc
     7141 ttttttcagg tcatagattg aaagattgta gctgtatttt ctcttccctc cggggggatt
     7201 ggccttttcc tgtgaaaaga ctatataatt cccgtccggc gaccatgagg gaagaagatt
     7261 cgcttcattg tcgccggtca gccgcctgac ttccccggta tagatattga agagatagag
     7321 gtcgccaatg ccctcaacca gccctgatac tgctatctca tcccctcccg gcgaccaggc
     7381 aggatttgaa aatgaagcta ctccggg
//
LOCUS       DAOSPX010000156         7381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9377_length_7381_cov_3.975566, whole genome shotgun sequence.
ACCESSION   DAOSPX010000156 DAOSPX010000000
VERSION     DAOSPX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9377_length_7381_cov_3.975566"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLZ75_08540"
     CDS             <1..343
                     /locus_tag="PLZ75_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72479.1"
                     /translation="DAMVSIYSPAAVENVWQSGKTDKNGYFLFKPDTEGEWIFVVDDQ
                     KGHMKRLAVNVQAGVSANEEDAPGESLAATATAHGGMSRTAGIITGLSIIFGITGIFY
                     GLRAKKAADSK"
     gene            351..1103
                     /gene="cbiQ"
                     /locus_tag="PLZ75_08545"
     CDS             351..1103
                     /gene="cbiQ"
                     /locus_tag="PLZ75_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOS72480.1"
                     /translation="MRHDLADRYSHLDSPVHRLDPRIKIIAALAGILIIVSEPRYGEP
                     IPFLFYSSVVIIVAAFSKVPPVYILKRLLIVSPFILMAAFFYPLSVYLSKSEFFLENR
                     QIIISAGLTILFKSFLSVIILIILGSTEKLHRLLHALRKLKMPSLITTISALLYRYVF
                     LIADEALKTSRARESRTPGHIRTGRIKAMGNQVAVIFLRSWERSQFIYKSMLSRGFKG
                     EFPDMEISRLGLTDIVFSGLFIILLLLIRIFL"
     gene            1105..1848
                     /locus_tag="PLZ75_08550"
     CDS             1105..1848
                     /locus_tag="PLZ75_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HOS72481.1"
                     /translation="MAAGIKIEKLSFSYTPGKEVLKSVSLDILPGEKFGIVGPSGSGK
                     STLLLHLNGILTGEGKIRIGEIPVAKSTLSEIRRLVGLVFQNPDDQLFNPTVEEDVAF
                     GPLNFGMAPEEVKDRVRETLESLKLNGFEKLTPHQLSQGERQRVALATVLSIRPGIIA
                     FDEPFSSLDPSMVIQLMEIISSLDSTLVITSQLVLPVIALCDRMAILKNGRIIACGPP
                     KQLARDTQLMKSSGLDLSFYRDMCRELWK"
     gene            complement(1885..2799)
                     /locus_tag="PLZ75_08555"
     CDS             complement(1885..2799)
                     /locus_tag="PLZ75_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOS72482.1"
                     /translation="MKADKKEAWAYAGLSLMTLIIGFSFIFVKIALRYASAIDLLAHR
                     FTAATAGLLLYYIFYRKSRPAISRENIPTLLLLSLFYPLLLFGFQTLGLKYTTASEAG
                     ILSAITPVITVILALIFLKEKNSLLQNICVLLSVSGVVYIMYRNGLNPVSGASMKGNM
                     LILLSVLSIAFYYVLGRKANRQYNSMDITLFMSVTACIVFNLAAITLHLKSGTLSHYF
                     GVFRYSGFLWPILYLGILSTFLTSFLGNNALSVIPAAQVSIFNNFSPVLSVFGGVLFL
                     NETLHVWHIIGGVMVLAGIIGVNIFRNK"
     gene            complement(2871..5573)
                     /locus_tag="PLZ75_08560"
     CDS             complement(2871..5573)
                     /locus_tag="PLZ75_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72483.1"
                     /translation="MKIFFRKSLILTALLIAAMATVSGQGLSDFSAEPSKAAGKSSIV
                     STNRFNGYTNHWQNYYYEWHRYGNMFKIALPETELAILQSKIDIAEDMGMPGLLMQEG
                     FISALLSEPYTTLQNPSPDELENTIRRQEGNILVITDPAGPLGRVLEEKVGGIFGWVK
                     DMNSYQLSDVDLEVVKAFFLTRGNRHLFVISSGSEEKARQLHSLTERTSAFMQSYNMF
                     KGWHGINTTLYIVTCSPGHPLEVIGKGMNEGNSWFIFDGYIAQYGKEKLEGWVNEVNL
                     PVVANTGYSPVFGCTDYKDLQVQNYMASRQYMIDYAHSKGGYAFREVFDPAADKFRYD
                     GYFVHPGNKEQIDNENVPFILKTGNLSDNLTSSMVLFIEKDSPLSNESIWDAIMNRRA
                     VAVLEKANMMGPAEYRNVLGLLYLDRIYLEEYFGDNLDISAQVEGYNLIVTMENHSSL
                     PLTGNLEVVTSDKVRAGEKLKENIVLPVNEKRQFVIPLQPLKEAMGNTNPVAVHFSYG
                     NRTKSTVTLLDMPPAISVNQLLYGHSPEIHFPVTVHNFSDSKSVAVEVNVFKKDNLRK
                     AVFRQSKVCEAEKAAFTTCDFNLRLGAGDYIVKTSALGMTAESQLGVGRAAGTTFVYE
                     VDLNSDGIPEYRMENDSVRITLLRTGARVIEYIVKSRNDNVLFRKWPEKNNRHDAPYR
                     MRGYYPYGGFEDFLGQASMETHRVYDAKIVREKGDYVQVEMETDYYGNHLKKIFTLYG
                     NSPLLEVRFELRFKNREANMLGPQPILELGRTHGPEDVFFVPSEKGLEEYRMLPEQKY
                     GWAFDLKEGWNAGYDTMEDIAFAGAFPVWQPVFLHMYFCTPGDILPPSQGNIEAPHYY
                     VEFQPWVRIPMNTIMYFSYYLWGSGGRWENAVEELKKRNLITVK"
     gene            5873..6451
                     /locus_tag="PLZ75_08565"
     CDS             5873..6451
                     /locus_tag="PLZ75_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOS72484.1"
                     /translation="MEELFKKLKRKVKHWWISLLVGLLAIFLAVWALVTPVETLTAMI
                     YVFIAMFFISGISDIGFALSNHNVMRGWGWGLMNGILEVIFGIILLIIPSTLLTTILL
                     YLVGFWVLFRSVWSVGEAIELQIIGVRGWGWFLALGILGIIASIIFIISPVFAGIFVV
                     SLISIALVFYGVFRIYLALGLRRINKFISRDN"
     gene            6488..>7381
                     /locus_tag="PLZ75_08570"
     CDS             6488..>7381
                     /locus_tag="PLZ75_08570"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOS72485.1"
                     /translation="MTKYSLIIILVFLFSCKGNKGEKDNDVFTENPAAEWITDARPLP
                     ESDSLFYSDQPAPLFRKEFKADRNVRSARLYIAAAGYYRVFVNGRSPDDNVLDPAWTN
                     FSKRIFYPEYDVTSLLQEGANCLAVSLGNGFFNPLPLRKWGHRNMRDVVPVGKPAFIA
                     ILRIKYGNGKTEEIVSDESWKYAYGPLLKNDVYIGVTYDAGKELPGWNKAGFNDSAWQ
                     TAVADTGPGGRLERSFFPPVRITEEITPVKIYSPSRGKYIADMGVNFTGTYRIRLSGN
                     RGDTVVFRFGERIYDDGSLNPM"
BASE COUNT         1949 a         1643 c         1693 g         2096 t
ORIGIN      
        1 tgatgcaatg gtaagcattt attctccggc cgctgtggaa aatgtctggc aatctggcaa
       61 aacggataaa aacggatatt ttctctttaa gccggatacg gaaggggaat ggatttttgt
      121 tgttgacgat cagaaaggac atatgaaaag attagcggtt aatgttcagg ccggcgtttc
      181 tgctaatgag gaggatgcac ccggagaatc cctggcagcc acagcaacgg cacacggtgg
      241 gatgagcaga accgcaggaa taataaccgg cttgtcaatt atcttcggta tcactggcat
      301 tttttacggt ctaagggcaa aaaaagctgc agacagtaaa taacaatgta ttgagacacg
      361 atttagcaga caggtacagc catctcgatt caccggtaca caggcttgat ccgcggataa
      421 aaataatcgc tgctcttgcg ggaattttaa tcattgtttc cgaacccagg tacggagaac
      481 caattccttt tttgttctat tcatccgtgg tgataattgt ggctgcgttc agcaaagtac
      541 ccccggttta cattctgaaa cgtttgctga tcgtttcacc ctttatctta atggctgcgt
      601 ttttctatcc actgtcggtt tatctttcaa agagtgagtt ttttcttgaa aaccggcaga
      661 taatcatttc ggccggatta accatacttt tcaaatcctt tctgtctgtt atcatcctta
      721 taatactggg ttccacggaa aagcttcaca gacttctgca tgctctacgg aaactgaaaa
      781 tgccttcgct gataactact atttcagccc ttttatatcg ttatgtattt cttattgccg
      841 acgaagccct taaaacatca agggcacggg aaagccggac tcccggacat atccgtaccg
      901 gcagaataaa agccatgggc aatcaggtgg ctgttatatt cctgcgcagc tgggagcggt
      961 cgcaatttat ttacaaatca atgctctcga gaggcttcaa aggtgaattt cccgatatgg
     1021 aaatttcccg tttaggactg acagatatcg ttttttccgg attattcatc atcctgctct
     1081 tgttaatcag aatattctta tagtatggcc gctggcataa aaatagaaaa actgagcttt
     1141 tcctatacgc cggggaaaga agtgttaaaa tcagtgtctc ttgacattct tcccggggaa
     1201 aagtttggaa tagtcggacc ttcaggttcg ggcaaatcaa ctcttttact gcatctcaat
     1261 ggtatattga cgggagaagg aaaaatcaga ataggagaaa taccagttgc aaaatcaact
     1321 ctttcagaaa taaggcggct tgtcggactt gtttttcaga accctgatga tcagcttttc
     1381 aatccaactg tagaggaaga tgtagctttt ggacctctta atttcggaat ggcgccggaa
     1441 gaagtgaaag accgtgtaag ggaaaccctt gaatcactta agctgaacgg ttttgagaaa
     1501 ctgactcctc atcaactgtc ccagggggag cgccagagag ttgccctggc cacagtattg
     1561 tcaatacggc ccgggataat agctttcgat gaaccttttt ccagtctcga tccttcaatg
     1621 gttattcagc tgatggaaat aataagttca ctggattcga cacttgtaat tacctcccag
     1681 ctggtgctgc cggttattgc attatgcgac cgtatggcaa tacttaaaaa tggccggata
     1741 attgcctgcg gccctccgaa acaactggca cgcgatacac aactgatgaa atcctcaggt
     1801 ctggatctaa gcttttaccg ggatatgtgc agggagctct ggaagtgatt ttccgttctg
     1861 aacaagtaag gattcgcttc tgaattattt atttctgaag atgtttacgc cgataattcc
     1921 ggcaagaacc attacaccgc cgataatatg ccaaacatgc agtgtttcgt tcaggaagag
     1981 gactccgccg aagacggata acacgggact gaaattattg aatattgaca cctgtgctgc
     2041 aggtatcacc gagagggcat tgttgcccag gaacgacgta agaaacgttg ataaaatgcc
     2101 aaggtaaagt attggccaga gaaatccgga atatctgaat accccgaaat agtgggaaag
     2161 agttcccgat ttcagatgta gggtaattgc tgcaaggtta aacacaatac aggcagttac
     2221 gctcatgaaa agggtgatat ccattgagtt atactgcctg ttggcctttc ttccgaggac
     2281 gtaatagaag gcgattgaca gtactgagag cagaatgagc atgttgccct tcatgctggc
     2341 cccgcttaca ggattcagtc cgttcctgta catgatatac actacccccg aaacagacaa
     2401 taaaacgcat atgttctgta ggaggctgtt cttttccttt agaaagatca gggccaggat
     2461 gacggtgatg acaggtgtta ttgccgaaag gatgcctgcc tctgaagcag ttgtatattt
     2521 taatccaagg gtttgaaatc caaaaagcaa cagcggataa aataacgaca gcaaaaggag
     2581 tgttggtatg ttttcgcggc tgatggcggg ccggcttttc ctgtaaaaaa tatagtacag
     2641 caacaacccg gctgtagctg ccgtaaagcg atgtgccagc agatcaatgg cagaagcata
     2701 cctcagggct atcttgacaa atatgaagga gaaaccgatg atcagtgtca tcagtgaaag
     2761 acctgcatat gcccatgctt cttttttgtc tgctttcatg ccccgtaaat gaacccgcaa
     2821 aaataacaca taataatacg ggttttaata tttctgtcct gtgatattct ttattttacg
     2881 gttataagat ttctcttttt cagttcctca acggcatttt cccatcgtcc gccgcttccc
     2941 cacagataat atgaaaaata cattatggta ttcatgggaa tccgtaccca gggctgaaac
     3001 tctacatagt agtgaggtgc ttcaatattg ccctggcttg gaggaagaat atccccggga
     3061 gtacagaagt acatatgcag gaagaccggc tgccatactg gaaatgctcc cgcgaaggca
     3121 atatcttcca ttgtgtcgta accggcattc cacccttctt tcaggtcaaa ggcccagcca
     3181 tatttctgtt cgggaagcat cctgtattct tccagtccct tttcagaagg tacaaagaag
     3241 acatcttccg gaccatgtgt cctgcccagt tccagaatgg gctgcggtcc cagcatattg
     3301 gcctccctgt ttttgaatct cagttcaaac cttacttcca gcaggggaga attgccgtag
     3361 agagtgaata tctttttcag gtggttgcca taataatcag tttccatttc cacctgaaca
     3421 taatcccctt tctccctgac aatttttgca tcataaaccc tgtgggtttc catactggcc
     3481 tgtcccagga aatcctcaaa gccgccatag gggtagtagc ccctcattct gtagggcgca
     3541 tcatgacgat tgtttttctc cggccatttt ctgaacagca cgttgtcatt cctgcttttc
     3601 acgatatatt caattaccct tgcacctgtc cgtaagagtg tgatcctgac agaatcgttc
     3661 tccatgcggt attcaggaat accgtcactg ttcaggtcaa cttcatagac aaaggttgtt
     3721 cctgccgccc ggccgacacc cagctgactc tccgcagtca tccccagggc acttgttttt
     3781 acaatgtaat caccggcacc gaggcgaagg ttaaagtcgc aggttgtgaa tgcagctttt
     3841 tctgcttcgc atacctttga ttgcctgaaa acagcttttc ggagattgtc ttttttaaac
     3901 acatttactt ccacggcaac agatttacta tctgaaaaat tgtgcacggt aacgggaaag
     3961 tgaatttccg gggaatgacc ataaagcagc tggtttaccg aaattgccgg gggcatgtca
     4021 agaagggtca cggtgctttt ggtccggttt ccgtatgaaa aatgaacagc tacgggattg
     4081 gtgttcccca tagcttcctt caggggctgc aggggaataa caaactgtct tttttcattt
     4141 accggcagga ctatgttttc cttcagtttc tcgccggccc ttaccttgtc tgatgtgacg
     4201 acctccagat ttccggtcag cggaagggaa gaatgattct ccattgtaac aatcaggttg
     4261 tacccttcaa cctgtgcgga tatatccaga ttatccccga aatactcttc gaggtatatc
     4321 ctgtcgagat aaagcagtcc cagcacattc ctgtattctg caggtcccat catgtttgcc
     4381 ttctccagaa cggccacagc ccgcctgttc atgattgcat cccatatcga ctcattgctc
     4441 agcggactgt ccttttcaat aaacaatacc attgatgatg tcaggttatc gctcaggtta
     4501 cccgttttaa gtataaacgg aacattttca ttgtctatct gttccttgtt gccgggatgc
     4561 acgaaatagc catcgtatct gaacttgtcg gctgcaggat caaacacttc ccggaaagca
     4621 taaccgcctt tcgaatgtgc atagtcgatc atatactgcc gggatgccat ataattctga
     4681 acctgcagat ccttatagtc cgtgcagccg aataccggtg aatagcctgt atttgccacc
     4741 acgggcagat tgacctcatt cacccagcct tcaagttttt cctttccgta ctgagctata
     4801 tatccgtcga aaataaacca tgagttgcct tcattcattc ctttcccgat cacttccagc
     4861 ggatgtccgg gtgaacaggt tacgatatac agtgttgtgt tgataccgtg ccagccctta
     4921 aacatgttat atgattgcat aaaggctgaa gtcctttctg tcagggagtg aagctgccgg
     4981 gctttttctt ccgatccgga tgatattaca aaaagatgcc tgtttcccct tgtaagaaag
     5041 aaagctttta ccacttccag atcaacgtca cttagctggt agctgttcat gtcttttacc
     5101 catccgaaaa tgccgccgac tttttcttca agcacccggc ccaggggtcc ggcagggtca
     5161 gttataacaa ggatatttcc ttcctgccgg cgtatagtgt tctccagttc atcaggggaa
     5221 ggattctgaa gagtagtgta tggttcgctg agcagtgcag agataaaccc ctcctgcatc
     5281 agcaggccgg gcatacccat gtcttccgca atatcaatct tgctttgcag tattgcaagt
     5341 tccgtttccg gcagggctat tttgaacata ttgccatacc tgtgccattc gtaataataa
     5401 ttttgccagt gatttgtata gccgttaaat ctgttggtgc tgacaatgga tgattttccc
     5461 gcagcctttg aaggttctgc actgaaatct gaaaggccct gtccagagac cgttgccatt
     5521 gctgctatca gcaaagctgt taaaatcagt gattttctga agaatatttt catgacggat
     5581 agattaattt actttttcaa atcaggtttt taagataatg aaaagttgat tgcctgcaag
     5641 accggcatgg gaaagacaaa aaaacaccct gtattttccg gcatgctgaa ccccttccga
     5701 gggagtagga taagtaaagg ggtgcggaat tatatcgtca gggaattatt tattgttact
     5761 attcagtata tttgttttaa cccgcaacca gggatttttt tattcaccaa cttttctggc
     5821 aatgagtcag agagtcattt gaatgtaatc actaaccaaa aaaatcttaa ttatggaaga
     5881 gctttttaaa aaactgaaac gaaaggtgaa gcattggtgg atctcgcttc ttgtggggtt
     5941 actggcaatt tttttggctg tatgggcact ggtaacccct gttgaaacat tgaccgccat
     6001 gatctatgtg tttattgcaa tgttctttat cagcggaatc tccgatattg gttttgcctt
     6061 gagcaaccat aacgtaatgc gcggctgggg atggggtctg atgaacggca ttttggaagt
     6121 gatcttcggt attattttac tgatcattcc gtcaacattg ctgacaacca ttctgcttta
     6181 tcttgtcggt ttctgggtac tgttccgttc cgtatggagt gtgggagaag ccatcgaact
     6241 gcagatcatt ggagtacgtg gctggggatg gttcctggcg ctgggcatcc tgggaatcat
     6301 tgcatcaata attttcataa taagtcccgt gtttgccgga atttttgtgg tgtcactgat
     6361 cagtatagcc cttgtgtttt acggggtatt ccgtatctat ctggctctcg ggctccggag
     6421 aatcaataag ttcatttcaa gggacaatta ggaaaaacag gaacgacgac aattaaagca
     6481 taataacttg acaaaataca gtctgataat cattctggtt ttcctgtttt cctgcaaggg
     6541 gaacaaaggg gaaaaagaca atgatgtatt tactgaaaac ccggcagctg aatggataac
     6601 tgatgcccgg cccctgcctg agagtgattc gctgttctat tcagatcagc ctgcaccact
     6661 gttccggaag gaattcaaag ctgacaggaa tgtccgcagt gcacgattat atattgcagc
     6721 tgccggctat tacagggttt ttgtcaatgg ccgttcccca gatgataatg ttctggatcc
     6781 tgcctggacg aatttttcaa agagaatctt ttatccggaa tatgatgtca cgtcactcct
     6841 tcaggaggga gccaactgtc tggcagtttc tctgggaaac ggttttttca atccccttcc
     6901 tctccgcaag tgggggcacc gtaatatgag ggatgttgta ccggtgggga aaccggcctt
     6961 catagccatc ctccgcatta agtacgggaa cgggaaaacc gaagaaatag taagtgatga
     7021 aagctggaaa tatgcttacg gaccgcttct gaaaaacgat gtgtatatcg gtgttaccta
     7081 tgacgccggg aaggaattgc cgggatggaa caaagccggt tttaatgaca gtgcatggca
     7141 gacggcagta gcggataccg gccccggagg caggctggaa agatcatttt ttcctcctgt
     7201 gaggattact gaagagatca caccggtgaa aatctattct ccttccaggg ggaaatacat
     7261 tgccgatatg ggggtaaact ttaccggaac atacaggatc aggctctcgg gcaacagggg
     7321 agatactgtt gttttccgct tcggagagag aatctatgac gacgggagcc ttaatcccat
     7381 g
//
LOCUS       DAOSPX010000157         7353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9423_length_7353_cov_3.874212, whole genome shotgun sequence.
ACCESSION   DAOSPX010000157 DAOSPX010000000
VERSION     DAOSPX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9423_length_7353_cov_3.874212"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /locus_tag="PLZ75_08575"
     CDS             <1..1799
                     /locus_tag="PLZ75_08575"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS72486.1"
                     /translation="VNSPGRYGNVTWIKGKTYYLLLDFENSFPTTHTFYIDHPESTNH
                     VAGNTEVFDRVSTLNYRRAIPVIFSEAGTIYSLSVCHEGGRGNMLMAVYSNSSGTPGA
                     RLGVTSQTPVNSSAGWQTVSLENPVSVSAGQTVWLAWIFENSVGLRYANGAPARAQSP
                     QSWSGGMPQNFGTASLDNYRYSVYCSYTPGETTTATLGNTDVYDLVSNLNYRRAIPVT
                     FTESGTIESLSICHEGGRGNVLMAVYSNSSGTPGSRLGVTSATPVSSSAGWQTISLLN
                     PVAVSTGQTVWLSWIFENSVGVRHTSGSPARAQSGESWSGGMPQSFGTASLGNYRYSV
                     YCTYIPGESSTRQLGNTDIYELVSNLNYRRAIPVTFTEAGIIESLSICHTGGSGNMLM
                     AVYSNSSGTPGSRLGVTSATPVNSSAGWQTVSLINPVSVSPGQTVWLSWVFEHSVGVR
                     HTKSPPARAQSGASWSGGMPQSFGTASLADFKYSVYCTYSVPGSDKNISVDEPENVDS
                     DLFPIEKEQVLIYPNPTDGEFTISWKNTYDHRLTITIYSVSGQLVKTVQTDPGISKII
                     LNLNGMKNGIYFIELKDRKNDIILNRSGVIKK"
     gene            1879..2184
                     /locus_tag="PLZ75_08580"
     CDS             1879..2184
                     /locus_tag="PLZ75_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72487.1"
                     /translation="MDQQERGRLLKQIMWDYNIPAKDVDALLSGKKETAGHYSQSTFF
                     KKVLESYSWYTVLQLYSPEEINNLLTEEIITGLRSPSLKKKYEFVKKRLQEIIPNSG"
     gene            complement(2586..3578)
                     /gene="nadE"
                     /locus_tag="PLZ75_08585"
     CDS             complement(2586..3578)
                     /gene="nadE"
                     /locus_tag="PLZ75_08585"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOS72488.1"
                     /translation="MNQKKPFSKDIILLGNIEYVVNEIVEKLRDDVLRKIRRTGGVVG
                     ISGGIDSSVCLALSARAFGPDKVLGIMLPEKDSSPDSEMLASELAAKFGVKAITENIT
                     GALNGFRCYERRDEAVKRIFPEYDPRTYKMKIAVKDTGLFSNLPPVFYLIIIDKEGNE
                     QEKILPVKEYLQIVASSNFKQRTRMSMLYHHAECLHYAVIGTPNRHEQEQGFFVKYGD
                     GGADVMPIGNLYKTQVYQLAEYLGVPGGIIERTPTTDTYSAEQTQEEFFYQLPFAQMD
                     RYWYAFEKGYPVTEVAEVMGETPERVEALFRGFERRRRTTEYLRMAPVRDYYGI"
     gene            complement(3666..5633)
                     /gene="asnB"
                     /locus_tag="PLZ75_08590"
     CDS             complement(3666..5633)
                     /gene="asnB"
                     /locus_tag="PLZ75_08590"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOS72489.1"
                     /translation="MCGIAGIIDFDGKAGLTAGLTSMLSAISYRGPDESGIYQSDNAV
                     LGHVRLSILDPETGQQPLSESGKRYSIVLNGEIFNYRELRADLEKKGFIFKTGSDTEV
                     LLQLYIHYGHECLGMLNGQFAFAVWDKHKEELFLARDRMGVRPLFYNITDGIFSFASE
                     IKALFELPFISRELRPESLSQVYTFWTTLTPYTAFRNIFELSPGHYLYFSQKGVKTGK
                     FWNPDFSNVNHRLKLADALERFNELFRDAVRLRLHADVKIAAYLSGGIDSSATVAYIR
                     ELENQSLDTFSIGFEEEDFDESNYQNEAVQYFGTQHKSIICSSETIADIFPKVVWHSE
                     IPLTRTAPAPMLILSQLVRDNNIKAVITGEGSDEILAGYDIFREAKIRRFWASQPDSK
                     TRPRLLSTLYPDIPHLRNASPNILRMFFGYKLNEVDNPFYSHLLRWNNANHIKKHLSA
                     ALKETLDDYSPLEELSDSLPPGFSEWDLLAKAQWLETTVFMSGYLLSAQGDRMAMANS
                     VEGRYPFLDHRVVEFCSTLPPRLKLNGLNEKFLLKKLMTNRIPESIVKRPKHPYRAPI
                     SSVFLAGNTPDYVKLMLSENYTRKAGIFDFDSVSSLLSKIEKTGVASEMDNMTITAVI
                     STHLLYDQFIENNKQEFRKGELKNLKIVRDI"
     gene            complement(5636..5893)
                     /locus_tag="PLZ75_08595"
     CDS             complement(5636..5893)
                     /locus_tag="PLZ75_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOS72490.1"
                     /translation="MAYDQTVIQEKIRQYIVEASHVDKEKIKDESLLFKEGYFDSMGF
                     ILLITFLEEEFKIKTSDSDLLEENFESINAMTGFVVNKLLS"
     gene            complement(5910..>7353)
                     /locus_tag="PLZ75_08600"
     CDS             complement(5910..>7353)
                     /locus_tag="PLZ75_08600"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOS72491.1"
                     /translation="QVTDLSYQGQQALQQMTQIENEMSTLQVQERYYNYVLEYLSKNE
                     DMAGLAPPSVANVVDPIMNSLILDLLSMNAERSTILSNNAEKNLFLGQLENKIRLQRQ
                     TIIENVKNNLNTLNLTQNELNYRAEKLSGQISRLPRTELNMVSMQRKFNLTDAIYTFL
                     LQKRSEAAIAMASNYPDYEILEPARDSTKKILSPKTMINWILAVFLAFIIPTLYIILR
                     NFFNEKISSVSDVEKLLKRPVLSLIYTNYYKTEAVVSEMPGSSIAESFRNLRSNLFLK
                     FRTEPLKVIMVTSSQPMDGKSFVSFNLAAAIASVGHKSILVDCDLRRPTLHEKIKTNN
                     SKGLSNYMVNNTPKEELIAKSGINNLSIIPAGPVLPNSSELIEAGVLDGLMDYLKDRF
                     EYVILDATPTGLVADAIPLIKYATHILLVCRNNHTRKDVFGDVLTMFRTNNIDNFDVV
                     FNDLTLKGSHYGRYDSYYKKEKSSLIKKLV"
BASE COUNT         2018 a         1571 c         1589 g         2175 t
ORIGIN      
        1 aagttaattc accgggccgc tatggtaatg taacatggat aaaaggcaaa acatattacc
       61 tccttctgga ttttgaaaat tcatttccta ccacacatac tttctatatt gatcatccgg
      121 aatccacaaa tcatgttgcc ggaaataccg aagtatttga cagggtgtca acccttaatt
      181 accgcagagc catacccgtt attttctcgg aagccggtac tatttatagt ctgtctgtct
      241 gccacgaagg aggcaggggg aatatgctga tggcggtcta ttcaaactct tccggaactc
      301 cgggagcaag gctcggcgta acctcacaga ctcctgtcaa ttcttcagcc ggctggcaga
      361 ctgtctctct ggagaatccg gtttctgtca gtgccgggca aactgtatgg ctggcatgga
      421 tattcgaaaa cagtgtaggg ttgaggtatg caaacggagc acctgcaagg gcacaatcac
      481 ctcagtcatg gtcgggaggg atgcctcaga attttggaac agcatcactg gataattaca
      541 gatattcggt ttactgcagc tatacacccg gagaaacaac cactgccacc ctcgggaaca
      601 ctgatgtata tgatcttgta tcaaacctta attaccgcag agccattcct gtaactttta
      661 cagaatcagg cacgattgag agtctttcaa tatgccatga aggaggcagg ggcaatgtgc
      721 tcatggctgt atattcaaac tcatcgggta ccccgggatc aagacttgga gtgacatcag
      781 ccactcctgt cagttcttca gccggctggc agaccatatc cctgctaaat cctgttgccg
      841 tcagtacggg gcagacagta tggctgtcgt ggatttttga aaacagtgtt ggagtaaggc
      901 atacaagcgg atcacctgcc agggcacagt ccggggaatc atggtccgga ggaatgcctc
      961 agagtttcgg tacagcatcg ctgggcaatt acaggtactc ggtttattgt acctatatac
     1021 ccggggaatc atcaacaaga cagcttggaa acacggatat ttatgaactg gtgtcgaatc
     1081 ttaattaccg gagagccatt cctgttactt ttacagaagc cggtataatt gagagtctct
     1141 ctatatgcca tactggcggc agtggcaata tgctcatggc agtatattca aactcatcag
     1201 gtactccggg atcaagactt ggagtgacat ctgctactcc ggttaattct tcagccggct
     1261 ggcagactgt ttcgctgata aatcctgttt ccgtcagtcc cggacaaacg gtatggctct
     1321 cgtgggtctt tgaacacagt gtgggtgtga gacatacaaa aagtcctcct gcaagggctc
     1381 agtccggtgc ctcatggtcc ggaggaatgc cccagagctt tggtacagca tctcttgccg
     1441 attttaaata ttcggtttac tgcacctatt cagttccggg cagtgataag aacatatctg
     1501 ttgatgaacc ggaaaatgtt gacagtgatt tatttcccat cgaaaaggaa caggttctga
     1561 tctatcccaa tccgaccgac ggggaattta ctatttcatg gaaaaacaca tatgatcaca
     1621 gactgacaat caccatttac agtgtaagtg gccagcttgt taaaacagtt cagacagatc
     1681 cgggcatcag taaaataata ttaaacttga atggcatgaa gaacggtatt tatttcattg
     1741 aactgaaaga cagaaagaat gatattatat tgaacagatc gggtgtcatt aaaaaataaa
     1801 tggagagtta ttgactttaa ttcctaattt tgtataaatc aattataggt caggagaata
     1861 aacgctaaag tttgacagat ggatcagcag gagagaggca gattattaaa acagataatg
     1921 tgggattata atattcctgc aaaggacgtt gatgcgcttc tgtcaggaaa aaaggagaca
     1981 gccggacatt atagtcagag tacatttttt aaaaaggtac ttgaatctta ttcctggtac
     2041 actgttctcc agctttattc accggaagaa attaataacc ttctgactga agaaattatt
     2101 accggattga gaagtccctc tcttaaaaag aaatatgaat tcgttaagaa aagattacag
     2161 gaaattatac caaattcagg ataagttctt aaaatggtgg agtaattttc attttccatt
     2221 ttatcttacc gggggaactg ctcttggaca ccccgttgaa tatcttgtca aataaacttg
     2281 gagccataat atcacgggaa gaacccaagg atatattcga tatagtccat atctcaataa
     2341 attattcatt taaaaaattc tcctgcaaat agccgatgat tttcttcttg gaaaggataa
     2401 ctcagtatgt ccgggaataa atgcaatagc caatgccaaa ccattaattt tttagtgtct
     2461 ttagtgatga acctcctgta tcatatttga aaaagtgtcc tgttgttcaa aaagtcattc
     2521 gaaaaaatgt aaaataaagc aaaaagtcct tcgaaaaagt gtgtcgttgt gcaaaaagcc
     2581 tgaagttata ttccgtaata atcccgtacg ggtgccatcc gcagatactc cgtcgttctc
     2641 cttcttcttt caaatccccg gaacagggct tccacacgtt caggggtttc acccatgacc
     2701 tcagcaactt cagtgaccgg atagcccttt tcaaatgcat accagtacct gtccatctgt
     2761 gcgaagggaa gctgataaaa gaattcttcc tgggtctgct cggcagaata ggtatccgta
     2821 gtgggtgttc tttcaataat acccccgggt actcccagat actctgccag ctgatatacc
     2881 tgtgttttat agagattacc tattggcata acatctgcgc cgccatctcc atatttgaca
     2941 aaaaatccct gttcctgctc atgcctgttg ggagtgccta taacagcata gtgaagacat
     3001 tctgcatgat gataaagcat tgacattctg gtacgctgct tgaaattgga tgaagctact
     3061 atctgcaggt attccttaac cggaagtatc ttctcctgct catttccttc cttgtcaatg
     3121 attatcaggt aaaacacggg aggcagattg ctgaataaac cagtatcctt tacagcaatt
     3181 ttcatcttgt atgtccgggg atcatattcc ggaaaaattc ttttcacagc ttcatcacgt
     3241 ctttcatagc atctgaagcc gttaagtgct ccggtgatat tttctgtaat ggctttaacc
     3301 ccgaactttg cggcgagttc agaagcaagc atctcactgt caggactgga atccttctcg
     3361 ggaagcataa tccccagcac tttgtcaggg ccgaatgccc gtgcggaaag agcaagacat
     3421 acagatgaat cgattcctcc gctgataccg acgacacctc ccgtgcgccg tattttccgg
     3481 agaacgtcgt cacgcagttt ttcaacaatt tcatttacaa catactcgat attgccgaga
     3541 aggataatgt cttttgaaaa aggttttttc tggttcataa ttatattttt tatttcataa
     3601 tatacatgca tttgatttcc tctgcaataa tcaatctgat tattatcagg cctttaatga
     3661 atctttcaaa tatcccggac tatcttcaga ttcttcaatt ctcccttccg gaattcctgc
     3721 ttattgtttt caatgaactg gtcatacagc agatgtgttg aaatgacagc tgtgattgtc
     3781 atgttatcca tctccgatgc aacaccggtt ttttctattt ttgaaagaag tgatgaaacg
     3841 gaatcaaaat caaatattcc ggctttgcgg gtgtagtttt cggataacat cagcttaaca
     3901 taatcgggtg tattccctgc aaggaataca ctgcttatag gtgccctgta aggatgtttc
     3961 gggcgtttta ctatactttc aggtattctg ttggtcatga gttttttcag caggaacttt
     4021 tcattgagac cattgagttt cagtctgggc ggcagtgttg aacagaattc gacaacacgg
     4081 tgatccagga acggatatct cccttccact gagtttgcca tagccattct gtcgccctgt
     4141 gctgacagca gataacccga catgaaaact gttgtctcca gccactgggc ttttgcaagc
     4201 agatcccatt cactgaaacc cggaggcaga ctgtcagata attcttcaag tggtgagtaa
     4261 tcgtcaagag tttcctttaa agcggctgac aggtgtttct taatatgatt cgcattgttc
     4321 cacctcagca gatgcgaata gaaaggattg tcaacttcat tcagcttata tccgaaaaac
     4381 atcctcagta tgttcggact tgcatttcgc agatgaggta tgtcggggta cagggttgaa
     4441 agaaggcggg gccttgtttt ggaatcaggt tgtgaagccc agaatctcct gatttttgct
     4501 tccctgaaga tatcatagcc ggcaagtatc tcatcagatc cctcaccggt gataaccgct
     4561 ttgatattat tatccctaac cagttgtgac aggatgagca tcggggctgg tgctgtcctg
     4621 gttaacggga tctctgaatg ccagaccact tttggaaaaa tatctgcaat ggtttccgag
     4681 gaacagatga tggatttatg ctgagtacca aagtactgaa cggcttcatt ctgatagttg
     4741 ctctcgtcaa aatcttcttc ctcaaaaccg atggagaatg tatcaagaga ttggttttcc
     4801 agttccctga tatacgctac ggtagcggaa gagtcaatgc ctccgctcag ataggcagca
     4861 atttttacat cggcatgaag gcgcagtctt actgcatccc tgaaaagttc attgaaccgt
     4921 tcaagggcat cagccagctt aagccggtga ttcacattac tgaagtccgg attccagaac
     4981 tttccggttt taaccccttt ctgactgaaa tagagataat gtccgggaga cagttcaaaa
     5041 atatttctga aagccgtgta tggtgtgagt gttgtccaga aggtatatac ctgtgaaagg
     5101 ctttcaggcc tcagttcccg tgaaataaag ggaagttcaa acaaagcctt gatttccgag
     5161 gcgaagctga aaataccgtc agtgatattg taaaaaagcg gtcgtacccc catccggtcg
     5221 cgtgccagga ataattcctc cttatgtttg tcccagactg caaaggcaaa ctgtccattc
     5281 agcatgccca ggcattcatg tccgtagtgt atataaagct gcaaaaggac ttccgtatct
     5341 gaaccggttt tgaaaatgaa tccttttttt tcaagatctg cacgtagttc cctgtagttg
     5401 aatatttccc cgttcaggac gatactgtac cttttacctg attctgaaag aggctgctgg
     5461 ccggtttccg gatcgagtat actgagtctt acatgtccga ggacagcatt gtctgactga
     5521 tagatgccgc tttcatcagg accgcgataa cttattgcgc tgagcattga agttaaaccg
     5581 gctgtcagac cggctttgcc gtcaaaatcg attatgccgg caatcccaca catgatcagc
     5641 ttagcagctt attgacaaca aaaccggtca tcgcattgat cgactcgaaa ttttcttcga
     5701 gcagatctga gtcgcttgtc tttattttaa attcttcttc aaggaaggtg atcaataaaa
     5761 tgaaacccat tgaatcgaaa tagccttcct tgaagaggag tgattcgtcc tttatttttt
     5821 ctttgtcaac atgagatgcc tctacgatgt actgtctgat tttctcctga ataactgttt
     5881 gatcgtaagc catacctgta tttttattgt taaactaatt ttttgatcaa tgatgatttt
     5941 tcttttttat aataactgtc atagcgtcca taatgacttc ctttaagtgt caggtcattg
     6001 aatacaacat cgaaattatc gatattgttt gtccggaaca ttgtaaggac atctccgaaa
     6061 acatctttcc tggtatgatt atttctgcag accagaagaa tatgcgttgc atattttatc
     6121 aggggaattg catcggcaac aagacctgtt ggtgttgcat ccagaatcac atattcaaat
     6181 ctgtccttca ggtaatccat tagtccatca agcacaccgg cctcaataag ctccgaactg
     6241 tttggaagaa ccggccctgc aggtataatt gaaagattat ttataccgga ttttgcaatc
     6301 agttcctctt tcggggtatt attaaccata tagttcgaca gtccttttga attattggtt
     6361 tttatctttt catgaagggt gggtcttcgc aaatcacagt cgaccagtat cgatttatgg
     6421 ccgacggatg ctattgccgc agcaagattg aaggaaacaa aactctttcc atccattggc
     6481 tgcgaggaag tgaccattat taccttaaga ggttcagttc tgaatttcag gaacaggttg
     6541 ctccggagat ttctgaatga ttcggcaatg gatgaacccg gcatctcgga tacaacggct
     6601 tcggttttgt aataattggt ataaatgagg ctcaggacgg gtcttttcag cagtttttcg
     6661 acatcgctga ccgagctgat cttttcgttg aaaaagtttc tgagaatgat atacagggtc
     6721 ggaataataa atgccagaaa cacagccagt atccagttaa tcattgtttt cggcgaaagg
     6781 attttttttg tggaatccct tgcaggttcc agtatttcat aatccggata attggaagcc
     6841 attgcaattg cagcttctga ccgcttctga agcagaaagg tgtatatggc atcggtcaga
     6901 ttgaatttac gctgcatgct gaccatgttg agctcggtac gcggaagcct tgatatctga
     6961 ccggaaagtt tctctgcccg gtaattaagt tcgttctgtg taagatttaa ggtgttgagg
     7021 ttattcttaa cgttttcaat aatagtctgc ctctgcaatc tgattttatt ctcaagctgc
     7081 cccaggaaga ggtttttttc cgcattattg ctcaatatgg ttgagcgttc tgcattcatg
     7141 ctcagcaggt cgagaatgag tgaattcatt atgggatcaa caacattggc cactgacggg
     7201 ggagccagcc ctgccatgtc ttcattcttg ctgagatatt ccagaacata gttgtaatat
     7261 ctctcctgaa cctgcagggt tgacatttca ttctctatct gtgtcatctg ctgcagagcc
     7321 tgctgaccct gatagctgag atccgtaacc tgg
//
LOCUS       DAOSPX010000158         7333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9460_length_7333_cov_4.972108, whole genome shotgun sequence.
ACCESSION   DAOSPX010000158 DAOSPX010000000
VERSION     DAOSPX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9460_length_7333_cov_4.972108"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3022
                     /locus_tag="PLZ75_08605"
     CDS             <1..3022
                     /locus_tag="PLZ75_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS72492.1"
                     /translation="RISSTGADHNTAIFLQAVYAMLGGIFLSTFDITAHSFYLARFDE
                     TDMARAFILSGIAGMLLAGLASLSGKRVSPDIHAIIILLFVSLTGLLLWILLIVMPGD
                     RNVALTFIAMWPLNGLVMSKLSGIAPVKQEQGCKRFQPGNSRTWIITGAMAGSFLIVL
                     LVNAGTDARHFLLIASVSILSAAVMRILNRRKNPEETEGDCGVKEPYRNRQTFAELVI
                     KNRLFRRISCYAILSVAAALFIRYSFMAETRLLYPSVEGMARFLGLFTGCMMIIALIL
                     SIFVYPGFLRNFRLRSAVIMPPVIIGGLTLAVLAAAMVSGNDPAKTGFSTFFVFLALS
                     RLFSGALRESAEYPSGDIIQRILGREERYSTDQAVYGSIKGGGMFITGLLLVLAGSAG
                     SANLVYFPVFLLLIVGLWMIAGSRLYSFYRKTVISFRETEELPDPGQELPDVTYCRRN
                     RVSARLEFENDFLGLITGDISPLERNRNQWYIERIIDHAEIKKDINLLPALKRIRSDS
                     SIDRDVRARSSDIIQDLELLSSGMGNRVGKLKAMLLLAEDRTPPVSEVLRYLRERDDD
                     LKVIALGIIKKFSLKELLPDVCACLENPRIRLHAENALNSFGGSADQALRRQYLLSSG
                     NTVAASTILRVLANNCSHENMEFLFGLLWTETRAIREEVTGSISFCNYRLSTGDREGL
                     IRLITDVAGIITWNISAGIAIAGMNGKKAADAMDQENKRWVRFLFSLLSVAYGSRPVR
                     QIEENIREGSRECIGHAREMMEILFDDQIKTRLTVLFDRISPGARLRTLFRFYPGEIP
                     QREDLFGTIINRDYNLLGVWLRACMIRQVSRISGDEMAETLVALLFSPEMILRQEAAK
                     LLARSESSFYRQAWDRIPEEMKNIIENIISGKTSTHELLYDKVVFLKSRLKTLPEEKL
                     ISLAEELGFPELLLPESLPENDGYILWECSGGTGFCETSIHFDNITSETALKKDDRFF
                     YVLPLNALTDHLRRYPEHSPEIYKLIDETERRNQ"
     gene            2997..6047
                     /locus_tag="PLZ75_08610"
     CDS             2997..6047
                     /locus_tag="PLZ75_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72493.1"
                     /translation="MKPKEETSKSNPFPGLRPFTPEESELFFGREKESEEVFSKLLRN
                     RFITVTGASGSGKSSLVYCGILPKIIEMGRKESSPWRIITFRPGNDPFESLARAMHEN
                     IAETGLKEVSVETLLSDLYRESDGISTALKKHIIRGYEKVLLVIDQFEELFRYRTSDT
                     GGTYGTDTGEFIENLVNAVSLIDSRVFTIVTMRADFMGECAYYQGLTQLINRSNFLIP
                     RMERENYRQVIEGPAKYCGISVDQSFVETLLDDLEDQSDQLPVLQHALMRTWNHWYEL
                     NDPDRPMGYAEYDSIGTMRNAMSRHADEAYGELDKRGREICRRMFRAITEKGPDNKGI
                     RRPTVFSSLQSIIDCSPEELAEVIGKFRQPSRSFLTPGPDVDLKDDTVIDLSHESLIR
                     LWDRLAVWVDEEAASIQMYLKLSETSSLYQHGKTGLMRPPDLQMAINWRDQEKPTLKW
                     ARRYDPAFERAMVYLRTSEKEYQDEEKRKLRLQKNKIKRSRIFTILFGGATIIALGLM
                     IAALIQKLSADRQKEFAESRQLLAEMDMRRADSAASTAITRWQQSESGAVAARKDAEE
                     ALRQKDLIQNRLFVARRNADSALKAFAEADQKIAEVTEGMNSVNRLRMISLGRSLSLR
                     SLQLNGQKDLQTLLAYQAYLFNLNNGGYENDADIFNGLYNVVKQYGEGFKYYNGHQGE
                     IKSIAFRPGTEEFYCSDSEKKILKWTLNGGSNDFREVYSGERVIRILAADPEGSWLAA
                     GEENSSIIMIPLKPGETRYKLSAHLAGINSLNFSADGKHLYSASYDGRVLKWDLNEKT
                     CINITPAQINVHTADISPGGKFIAGITAEGSVTVWNPDDIQEHKWIQSERKNITVVRF
                     RKDDRTLAIGNTEGFVELWDALSGKRLSGTRAHEGRINDIRFNEELNQMATAGNDSCI
                     RIFDISDMAGLSVLPVVFADNGGTVTALAFSNNGQQIVSGIMGREMNLASRPASAGFM
                     VQNICTLVTRNLTEDEWNTWVDRDIPREEACSDIDFSIKIREVK"
     gene            6182..>7333
                     /gene="gpmI"
                     /locus_tag="PLZ75_08615"
     CDS             6182..>7333
                     /gene="gpmI"
                     /locus_tag="PLZ75_08615"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOS72494.1"
                     /translation="MNNRKTLLMILDGWGIGDGSKADVISTVPTPNLDELKKKYPSSR
                     LFASGENVGLPDGQMGNSEVGHLNIGAGRIIYQDLVKINLECKTGEIRKNKILTEAFS
                     YARDRDKQVHFMGLLSDGGVHSLDRHLYYLCDMTMDYNIRNVFIHGFGDGRDTDPHSG
                     KGYMKNLLDHLKQSNGKVASFTGRYYAMDRDKRWERIKRAYDVIVNGIGNPAENILDA
                     MQKSYDEGITDEFMEPIVVVDDSGKPVGQIREGDVVVFFNFRNDRAKELTIALTQKDM
                     PEHGMKTIPLYYCTMTPYDATFTGLHVIYPKENAGNTMGEVLANAGKKQLRIAETEKY
                     AHVTFFFSGGREQVFENEERTLIPSPKVATYDLQPEMSAYLVKDAVISAI"
BASE COUNT         2096 a         1570 c         1948 g         1719 t
ORIGIN      
        1 cagaatcagc agtaccgggg ctgatcataa cacggcaata tttttgcagg cagtgtatgc
       61 catgcttggg ggtatcttcc tgtccacctt cgatatcacg gcacattcat tctacctggc
      121 gcgctttgat gaaacagaca tggcgcgggc gtttattctc tcgggaatag ccggaatgct
      181 gcttgcaggc ctggcatcat tgtccgggaa aagggtaagt ccggatatac atgccattat
      241 cattctcctg tttgtatcat taaccggttt attgctctgg atcctgctga tcgtaatgcc
      301 gggggaccgg aatgtggctc tgacattcat tgcaatgtgg ccccttaacg gactggtgat
      361 gtcgaaatta agcgggattg ctccggttaa gcaggaacag ggctgtaaaa gatttcagcc
      421 gggaaattcc cggacatgga taataacggg tgccatggcg ggcagttttc tgatagtcct
      481 gctggtcaat gccggaacgg acgccaggca tttcctgctt attgcttccg tttcaatcct
      541 ttcggcagca gttatgcgga ttctgaaccg gaggaaaaat ccggaagaaa cagaaggcga
      601 ctgcggtgtg aaggaaccgt accgtaacag acagacattt gcagaactcg tgataaaaaa
      661 ccgtctgttc agaagaataa gctgctatgc cattctctcc gttgcagccg ccttgtttat
      721 aagatattca tttatggcgg agaccaggct gctgtatccc tcagtggaag ggatggcgcg
      781 tttcctgggc ctgtttacgg gctgcatgat gattatcgca ctgatccttt caatatttgt
      841 ttatccgggt ttcctcagga atttcaggct ccgctcagct gtaataatgc cgccggtgat
      901 tatcggggga cttacactgg cagtgcttgc cgcggcgatg gtctccggaa atgatcctgc
      961 aaaaaccgga ttcagtacat tttttgtctt tcttgccctg agcaggcttt tttcaggagc
     1021 tctcagggaa tcagctgaat atccgtcagg tgatataata cagcgtatcc ttggccggga
     1081 ggaaagatat tccacagatc aggcagtcta tggcagcata aagggaggag gcatgtttat
     1141 cacgggactg ctgcttgttc ttgccggatc ggccgggtcc gcaaacctgg tttatttccc
     1201 ggttttcctt ctgcttattg ttgggttgtg gatgattgca ggatcaaggc tctactcatt
     1261 ttacagaaaa accgttatca gtttcaggga aacggaggaa ctgccggatc cgggacagga
     1321 attgccggat gtcacatact gccgcagaaa cagagtctct gcccgtctgg aatttgaaaa
     1381 tgatttcctg ggcctgatta ccggagatat cagtcctctt gaaagaaacc ggaatcaatg
     1441 gtacatagaa aggataattg atcatgcaga aataaagaaa gatatcaatc tgctgcctgc
     1501 cctgaaaagg ataagatcgg attcgtccat agatcgggat gtcagagccc gcagttcaga
     1561 tattatccag gatctggaac ttctttcctc aggcatggga aacagggtag ggaagctgaa
     1621 ggcaatgctg cttctggcag aggaccgtac accaccggtt tctgaagtac tcagatattt
     1681 acgtgaaagg gatgatgacc tgaaagtgat agcactgggc attatcaaaa agttcagcct
     1741 gaaggaattg cttcccgatg tctgcgcatg cctggaaaat ccccgtatac ggctgcatgc
     1801 ggagaatgcc ctcaacagct tcgggggaag tgcagatcag gctctccgca ggcaatacct
     1861 cctgtcatcc ggcaatacgg tggcagcatc taccattctc agagtgcttg caaacaactg
     1921 cagccatgaa aatatggaat ttcttttcgg attgctatgg actgaaacac gtgccatacg
     1981 ggaggaggtg accggaagta tttcattctg taattacagg ctcagtacgg gtgacaggga
     2041 aggactgatc aggctgataa cggatgtcgc cggaataatt acctggaaca tatctgccgg
     2101 tattgcaatc gcggggatga acggcaaaaa agccgcggac gccatggatc aggaaaacaa
     2161 aagatgggtc aggttcctgt tcagcctgct ttcggtggct tacggcagca ggcccgtcag
     2221 acagatagaa gaaaacatca gggaaggcag ccgtgaatgt atcgggcatg ccagggaaat
     2281 gatggaaata ctctttgacg accagattaa aacaaggctt acggtactgt tcgaccgcat
     2341 atcccccggt gccaggctca ggacactgtt caggttctat cccggagaga taccgcaacg
     2401 ggaagacctt ttcgggacaa taataaaccg cgactataat ttgctgggcg tctggctcag
     2461 agcctgcatg atcaggcagg tttcccgcat aagcggcgat gaaatggcag aaacacttgt
     2521 tgcacttctg ttcagtccgg aaatgatact ccgccaggaa gctgcaaaat tgcttgcacg
     2581 ttccgaaagc agtttttaca ggcaggcatg ggacagaatt ccggaagaaa tgaagaatat
     2641 tattgagaat atcatcagcg gaaagaccag cacacatgaa ctgctttacg acaaggtagt
     2701 tttcctgaaa tcccgtctga aaacccttcc tgaggaaaaa ctgatttccc ttgcagaaga
     2761 acttggattc ccggaattgc tgctgccgga atccctgccg gaaaacgacg gatatatcct
     2821 gtgggaatgc agcggcggga caggcttttg cgaaacaagc atacattttg acaacataac
     2881 cagtgagacg gcattgaaaa aagatgacag gttcttttat gttcttcccc tgaatgccct
     2941 tactgatcac ctgagaagat atcctgaaca ttcaccggaa atttataagc taatagatga
     3001 aaccgaaaga agaaaccagt aagtccaatc cctttccggg gctcaggccc ttcactcctg
     3061 aagaaagcga actcttcttc gggcgtgaaa aggaaagtga ggaagtattc agcaagcttt
     3121 taaggaaccg ttttattacg gtcacgggag cttcgggaag cggtaaatcc tctctggttt
     3181 actgcgggat cctgccgaag ataattgaga tgggcaggaa ggaatcttca ccatggcgga
     3241 taataacctt caggccgggc aacgaccctt tcgagagcct ggcacgggca atgcatgaaa
     3301 acattgcgga aacagggctg aaggaagtgt ctgttgaaac cctgctgtct gatctctacc
     3361 gcgaatccga cggcatttca acggcactga aaaaacatat aatcagggga tatgaaaaag
     3421 tgctgctggt tatagatcag tttgaggaac tcttccggta ccggacttcc gataccggag
     3481 ggacatacgg aacagatacc ggggaattta tagagaatct ggtgaatgct gtcagcctca
     3541 ttgattccag ggtttttaca attgtgacca tgagagctga ttttatggga gaatgtgcat
     3601 attaccaggg tctcacgcaa ttgataaaca gaagcaactt tctcattccg agaatggaaa
     3661 gggaaaatta ccgtcaggtt atcgagggac cggcaaaata ttgcggaatc tctgttgacc
     3721 agtcatttgt tgaaacatta cttgatgatc tggaggatca gagcgatcag cttcctgtat
     3781 tgcagcatgc actgatgcgg acctggaatc actggtatga gctcaatgat cctgacaggc
     3841 cgatgggata tgctgaatat gactcaatag gtaccatgag aaatgccatg tcgcgtcatg
     3901 ctgatgaggc atatggcgaa ctggacaaaa ggggaaggga aatttgcagg agaatgttca
     3961 gggcaataac ggaaaagggt ccggataaca agggtatcag gcgcccgaca gttttttcct
     4021 cactgcagtc catcattgac tgcagcccgg aggaactggc tgaagtgatc gggaaattca
     4081 ggcagccatc ccggtcattc ctcacgccgg gccctgacgt ggatttaaag gatgacacgg
     4141 ttatcgatct gtcgcatgaa agccttatca gattgtggga caggctggcc gtatgggttg
     4201 atgaagaagc tgcttcaatt cagatgtatc ttaagctgtc ggaaacttct tccctgtacc
     4261 agcatggaaa aaccggcctt atgaggcctc ccgacctgca aatggcaatt aactggcggg
     4321 atcaggaaaa gcccacactg aaatgggcca ggagatatga tccggccttt gaaagggcaa
     4381 tggtttatct gcggacaagc gaaaaggaat accaggatga ggaaaaaaga aaactcaggc
     4441 ttcagaaaaa caagataaaa agaagcagga tatttaccat ccttttcggg ggtgcaacaa
     4501 ttatagcact cggactgatg attgccgcac tgatacagaa attatcggct gacaggcaga
     4561 aagaatttgc cgaatccagg cagctattgg ccgagatgga tatgagacgg gctgattcgg
     4621 ctgcaagtac ggcaataacc cgctggcagc aatctgaatc cggggcagtg gctgcaagga
     4681 aggatgccga ggaagctctc cggcagaaag atctgataca gaaccgcctg tttgttgccc
     4741 gcagaaatgc cgattcggcc ctgaaagcct ttgccgaagc ggatcagaaa attgcggaag
     4801 tgactgaagg gatgaattcc gttaacaggc tgaggatgat atcgctggga aggtctttat
     4861 cgcttaggtc actgcagctc aacgggcaga aagatctgca gaccctgctc gcatatcagg
     4921 catacctgtt caatctgaat aacggaggct acgaaaatga tgcggatata ttcaacggtc
     4981 tttacaatgt tgtaaaacaa tacggagaag gattcaaata ttacaacgga catcagggtg
     5041 aaataaaaag cattgccttc cgtcccggga cggaagaatt ttattgctct gacagtgaga
     5101 agaaaattct gaagtggaca cttaacggcg gttcaaatga tttcagggag gtctacagcg
     5161 gtgaaagggt gatacggata ctggccgcag atcctgaagg atcatggctg gcggcgggag
     5221 aggaaaactc atcaattatc atgataccac tgaaaccggg agaaacgaga tataagctct
     5281 cggcccacct tgcaggaatc aattcactga acttctctgc ggacggaaag catctttatt
     5341 ctgcttcata tgacggaaga gtgctgaaat gggatcttaa tgaaaaaaca tgcataaata
     5401 ttaccccggc tcagataaat gttcacacag cagatatttc tccgggcggg aaattcattg
     5461 ccgggattac tgctgaaggc agtgtgactg tctggaatcc tgatgacatt caggagcata
     5521 aatggataca aagtgaaagg aaaaacatta ctgtggtcag gtttaggaag gatgacagaa
     5581 cccttgcaat cgggaataca gaaggctttg tggagctttg ggatgccctg agcgggaaaa
     5641 ggctgtcggg caccagggca catgagggcc ggatcaatga tatcagattc aatgaggagc
     5701 tgaatcagat ggccacagcc ggaaatgaca gttgcatcag gatttttgac ataagcgaca
     5761 tggccggcct gtcagtgctt cccgtagtgt ttgccgacaa cggcggaaca gttacggccc
     5821 tggcattcag caataacgga cagcagatag tatcaggaat aatgggcagg gaaatgaacc
     5881 ttgcaagccg gcccgcaagt gcaggattta tggttcagaa catatgcacg ctggttacac
     5941 ggaaccttac ggaggatgag tggaacacct gggttgacag ggatattcca agggaagagg
     6001 catgcagcga tattgatttc agtataaaga tcagggaagt gaaataaaat agtgctgaat
     6061 aacacaaatt atccgggttg aatccctttt tggctaaatt tgcaccctgg attccggaaa
     6121 gggtctgtcc ggcctttttc ggatgccgca ataaaaacga tttattaatc cggaaaataa
     6181 gatgaataac aggaaaaccc ttctgatgat tctcgacggc tggggaatag gagacggaag
     6241 caaggctgac gttatcagca ccgtaccgac gcccaacctg gatgagctaa agaaaaaata
     6301 tcccagttcc aggctgtttg cctcaggcga gaatgtggga ctgcctgacg ggcaaatggg
     6361 aaattcagaa gtcggccatc tgaacatagg tgcaggaaga ataatttacc aggatctggt
     6421 taaaataaac ctcgaatgca agaccgggga aatacgtaag aacaagattc tcaccgaagc
     6481 tttttcatat gcacgcgaca gggataaaca ggttcatttc atgggcctgc tgtctgacgg
     6541 cggtgttcac tctcttgacc ggcatctgta ctatctgtgc gacatgacca tggattacaa
     6601 tatcaggaat gtttttatcc acgggttcgg agacggacgg gatactgacc cgcacagcgg
     6661 caagggctac atgaagaatc ttcttgatca tctgaaacag tcaaacggga aagtagcctc
     6721 gttcaccgga cggtattatg caatggacag ggataagaga tgggaaagga ttaaaagagc
     6781 ttatgatgtg atcgttaacg gcataggaaa tcctgctgaa aatatcctgg atgccatgca
     6841 gaaatcctac gatgaaggga taactgatga attcatggaa ccaattgtgg tggttgacga
     6901 ttccggaaag cctgtcggac agatcaggga aggagatgtt gtagtatttt tcaatttcag
     6961 gaatgacagg gcgaaggagc ttacaatagc actcacacag aaggacatgc ctgaacatgg
     7021 catgaaaacg atacctctgt attactgcac aatgactccc tatgatgcta cattcacggg
     7081 tctgcatgtc atatatccca aggagaatgc aggcaataca atgggagaag tgcttgccaa
     7141 tgcgggaaag aaacagctga gaatagctga aacggagaaa tatgcacatg tcacgttttt
     7201 cttttccgga ggcagggaac aggtattcga gaatgaagaa cgcacactta tcccgtcgcc
     7261 gaaagttgcc acctatgatc ttcagcccga aatgagtgca taccttgtta aagatgccgt
     7321 tatcagtgcc att
//
LOCUS       DAOSPX010000159         7303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9512_length_7303_cov_3.826849, whole genome shotgun sequence.
ACCESSION   DAOSPX010000159 DAOSPX010000000
VERSION     DAOSPX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9512_length_7303_cov_3.826849"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="PLZ75_08620"
     CDS             <1..1496
                     /locus_tag="PLZ75_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOS72495.1"
                     /translation="TEEYRTGTYREFTRDVLPYIAGAGYNTIQLMAIQEHPFYGSFGY
                     HVSSFFAVSSRFGTPEDLRELIDTAHGLGIRVIMDLVHSHSVKNINEGLALFDGSPGQ
                     YFHTNERRNHIAWDSLCFDYGKDNVIHFLLSNCRYWIDEYGFDGFRFDGVTSMIYYDH
                     GLGKNFTEYSEYFDGGQDIDALIYLYLANKMIHEIKPHALTVAEEMSGMPGLAAHTSA
                     GGYGFDYRLSMGVPDFWIRLVKEIADEKWDMGTLMHELTQHRPEEKVISYCESHDQAL
                     VGDKTLIFRMIDAEMYTGMNKSHQSLVIDRGIALHKMIRLITLATAGGGYLTFMGNEF
                     GHPEWIDFPREGNNWSYKYARRQWSLLKNESLKYGYLAAFDREMLTLQNRYGILEHPL
                     SRIYENSSDKVIAFSRGELLFVFNFHPGNSFTDYGIPVQGKFRIILDSDDPAYGGFNR
                     IDRNGLYLSIRKAARQTLGTPYYLYLYLPSRTAIVLKREPVRSVIDI"
     gene            complement(1493..5716)
                     /gene="glgP"
                     /locus_tag="PLZ75_08625"
     CDS             complement(1493..5716)
                     /gene="glgP"
                     /locus_tag="PLZ75_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOS72496.1"
                     /translation="MKINFLFETSWEVCNKVGGIHTVISTKALNILEELGDNYILIGP
                     DVWREESENPEFLQDDSLFEEWKGKALSEDLRIKTGRWNIAGKPIVFLIDFTPYFGQQ
                     NEIFARFWETYKLDSITGQWDYIEPALFGYAAAKVIESFTSFYQEHHDIIAQFHEWMT
                     GTGVLYLEHNAPWVATAFTTHATVLGRSIAGNNRPLYGKMKEYNPGQIAREFNVAAKQ
                     SLERITAAEADVFTTVSGITSKECSHFLGKDVDIVTPNGFEDSFVPDEKHFAERRNTA
                     RLKLKAVAEAVLGYGISDDAVFIANSGRYEFRNKGLDIFIDALGRLNKKEKPKRECVA
                     FIMMPAYHMGPRQDLMEILYNDAEEHGGDRYLTHYLHYPATDPVIQRINANQLDNNKE
                     SQVKIIFAPSYLNGNDGIFNLPYYDLLIGFDLSAFPSYYEPWGYTPLESLMFSIPTIT
                     TSLSGFGLWVREYFKDPGNGIAVIERTDNNEDQVVSDIMEFMQMFINLSDDEINKARL
                     KAHEISRIAMWDTLVKYYFSAYEKALLKSSERREEPREFARFVEEPGLVVRKPHQLPV
                     WKDIYIQSDVPQKLSALKDLANNLWWSWNSDAESIFRRMDPSLWEEIRHNPKILLEKI
                     DYKRLLVLEDDDDFVKDLRKVDKAFRDYMSRPDDNQKPSVAYFSMEFGIHPSLKIYSG
                     GLGILAGDYLKEASDSNLKIIGVGFLYRYGYFRQKLGPKGEQLTIYEAEDFTSLPIRP
                     VKDKNDNHLRVAVVWPGRTVMIRVWEAKIGKVSLFLLDTDFEDNSAADRSITHYLYGG
                     DHENRLKQEIILGIGGIRALDAMGIKPDLYHSNEGHSAFISLERLRTMIETHHLTFHE
                     ALEAVRSSTLFTTHTPVPAGHDAFDEDMLRKYISHYHTRLNISWEELVALGRCEGDPD
                     RKFNMSFLATRMSQEVNGVSKLHGEVSQGMFNKLWPGYLQEELFIGYVTNGVHHATWT
                     ANPWKEVWKEITGSSSFDQTDRSKWEKLYKVDDKRIYEIRRQLKKTLFNNIRKRLQTD
                     MIDKHVSPRTLINISTHLDENALTIGFARRFATYKRASLLFRDLDRLERIVNNPDRPV
                     QFIYAGKAHPHDGGGQDLIRRVFEVSQMPQFAGKIVFLENYDIELAKYMVQGVDIWLN
                     TPTRPLEASGTSGEKAVMNGTLHFSVLDGWWVEGYRAYAGWALPKKKTFANQYLQDDV
                     DAETIYNMLEYEIVPAYYSYDDKGVPVEWISHIKNTMVKVAPEFTMKRQLDDYYDKYY
                     SRLYERNKYLTANNFEKVKDLSAWKKRITAEWDNIEVLNYSFEEPDGNIYHSGHDYKA
                     EIALDIKNIPKENVGVEFIVTHLSKEGRHEFVNSQEFTLVSCKGGKCLYRVKLIPEKA
                     GAFFYGIRIYPKRGDLPHKQDFYLLRWID"
     gene            complement(6072..7271)
                     /locus_tag="PLZ75_08630"
                     /pseudo
     CDS             complement(6072..7271)
                     /locus_tag="PLZ75_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
BASE COUNT         1721 a         1822 c         1628 g         2132 t
ORIGIN      
        1 ccactgaaga ataccgtacc gggacctaca gggaattcac aagggatgtt ctgccctaca
       61 tagccggtgc aggatacaac accatccagc tgatggccat tcaggagcat cctttctacg
      121 gatcctttgg ctatcatgtt tccagcttct ttgcagtctc ttcccgcttc ggcaccccgg
      181 aagatctcag ggaactgatt gacacagccc atggcctcgg aatcagggtg atcatggatc
      241 tggtccattc ccattcagta aagaatatca atgagggact ggctcttttt gacggatctc
      301 ccggccagta cttccacaca aacgagagaa ggaaccatat tgcatgggac tccctgtgct
      361 tcgactacgg aaaggacaat gtgatccatt ttctgctctc caactgccgg tactggattg
      421 atgaatacgg gtttgacggc ttcaggttcg acggtgtaac gagcatgatc tactatgatc
      481 acggattagg aaaaaatttc accgaatatt ctgaatattt cgacgggggc caggatattg
      541 atgcactgat ttatctttac ctggcaaaca agatgatcca cgaaatcaag cctcatgccc
      601 tcactgttgc tgaggagatg agcggaatgc ccggactggc ggctcataca tcagccggag
      661 gctatggatt cgattacagg ctgtcaatgg gggttcccga tttctggatc aggctggtca
      721 aggaaattgc cgatgagaaa tgggatatgg gcacgctgat gcatgaactt acccagcacc
      781 gtcccgagga gaaggtgata tcatactgtg aatcacatga ccaggccctg gttggtgaca
      841 aaacgcttat cttcagaatg atcgatgccg aaatgtatac cggcatgaac aagtcgcacc
      901 agagcctggt aattgaccgt ggcattgccc tccacaagat gataagactg attacccttg
      961 ccactgccgg aggcggttac cttactttca tgggaaatga gttcggacac cctgaatgga
     1021 ttgatttccc gagggaagga aacaactgga gttataaata tgctcgccgg caatggagcc
     1081 tgctgaaaaa tgaatcactc aaatatggct acctggccgc ctttgacagg gaaatgctca
     1141 ctctccagaa ccgttacggg atccttgagc accccctctc aagaatatat gagaactcct
     1201 ccgacaaggt cattgccttt tcaaggggag aactcctgtt cgtattcaat ttccatcccg
     1261 ggaattcatt caccgactac gggataccgg tgcagggcaa gttcagaata attctggatt
     1321 ccgacgatcc tgcctacggc ggattcaaca ggatagacag gaacggcctc tacctgtcta
     1381 tcagaaaggc agcaaggcag acactgggta caccttacta cctttatctt tatcttccgt
     1441 caagaacggc cattgtcctt aaaagggagc cggtcaggag tgtgatagat atttaatcaa
     1501 tccacctcag caggtagaaa tcctgcttgt ggggcaggtc tccacgcttg ggatagatcc
     1561 ttattccata gaaaaatgct cctgcctttt caggaatgag cttcaccctg taaagacatt
     1621 ttcccccttt gcagctaaca agggtgaatt cctgactgtt cacgaactca tgtcttccct
     1681 ctttgctcag gtgtgtcaca atgaactcca cccccacgtt ttcctttggt atatttttga
     1741 tgtccagagc tatttcagcc ttgtaatcat gccctgaatg gtaaatattt ccgtcaggct
     1801 cctcaaagct gtaattcaga acctcaatat tatcccactc tgccgtgatt ctctttttcc
     1861 aggcagaaag gtcttttacc ttctcgaaat tatttgcggt cagatattta ttcctttcat
     1921 aaagcctgga atagtattta tcataatagt catccagctg gcgtttcatt gtgaattcag
     1981 gtgccacttt caccattgtg ttcttgatat ggcttatcca ctccacaggc acacccttgt
     2041 catcatagga ataatatgcc ggcactattt catactcaag catattgtag atggtttcgg
     2101 catcaacatc atcctggagg tactggtttg caaatgtctt tttcttcggc agcgcccagc
     2161 ctgcatatgc cctgtagccc tctacccacc agccgtccag cacgctgaaa tgaagggttc
     2221 cgttcataac agccttctcg ccgctggtac cggaggcttc aagcggtctg gtaggagtat
     2281 tgagccagat atccactccc tgaaccatgt attttgcaag ctctatatca tagttttcaa
     2341 gaaatacgat cttaccggca aactgcggca tttgtgaaac ttcaaatact cttctgatca
     2401 gatcctggcc tcctccgtca tgcggatgtg ccttgcccgc gtaaataaac tgaacgggcc
     2461 tgtccgggtt gtttacaatc ctttcaagcc tgtcaagatc cctgaacagc agggaagctc
     2521 tcttgtaagt agcgaaacgc ctggcaaaac cgattgtaag agcgttctca tcaagatgag
     2581 tgcttatgtt gatcagtgtt ctcggactga cgtgcttgtc tatcatatca gtctgaagac
     2641 gcttcctgat attgttaaac agggttttct tcagctgcct tcttatttca tagatcctct
     2701 tgtcatcaac cttgtaaagc ttttcccact tgctcctgtc ggtctggtcg aaggatgatg
     2761 acccggttat ttccttccat acttccttcc acggattggc tgtccaggta gcatgatgca
     2821 caccatttgt aacatatcct ataaacagct cttcctgaag gtatcccggc cacagcttgt
     2881 tgaacatacc ctggcttacc tccccgtgaa gcttgctcac tccgtttacc tcctgcgaca
     2941 tgcgtgttgc aaggaagctc atgttgaatt tcctgtcggg atccccctcg catcttccga
     3001 gcgccaccag ttcctcccag cttatattga gccttgtatg gtaatgtgat atgtatttcc
     3061 tgagcatatc ctcatcaaat gcatcatgtc ctgccggaac gggagtatgt gttgtgaaca
     3121 gggttgatga tctcacggct tcaagtgcct catggaaggt aagatggtgg gtctctatca
     3181 tcgttctgag gcgttcaagg cttataaatg ccgaatgccc ctcgttgctg tgataaaggt
     3241 cgggtttgat acccatggca tcaagggccc tgatacctcc gatacccagg ataatctcct
     3301 gtttcagcct gttctcatgg tcgccaccgt acagataatg tgttattgac ctgtcggcag
     3361 ctgaattgtc ttcaaaatca gtgtcgagca ggaagaggct taccttgccg atcttggcct
     3421 cccacaccct tatcataacg gttcttcccg gccatacaac agccaccctg agatgattgt
     3481 cattcttgtc cttgaccggt ctgatgggaa ggcttgtaaa atcttccgcc tcataaattg
     3541 tgagctgctc tcctttcggt cccagcttct gtctgaagta gccataacgg taaaggaatc
     3601 ccactcctat tatcttcaga ttggaatcgc ttgcctcctt cagataatcc ccggccagaa
     3661 taccaagtcc gcctgaatat atcttaaggg agggatgtat tccgaattcc atgctgaaat
     3721 aggctactga aggcttctga ttgtcatcgg gccggctcat gtaatccctg aaggccttgt
     3781 ccaccttcct gaggtccttc acaaaatcat catcatcctc gagtaccagc agccttttgt
     3841 aatcaatctt ctcaagcaga atcttgggat tatgccttat ctcttcccag agggaagggt
     3901 ccattctcct gaatattgat tccgcatcac tgttccatga ccaccacagg ttgtttgcca
     3961 gatccttcag tgctgaaagc ttctggggca catcgctctg aatgtagata tccttccaga
     4021 cgggaagctg gtgcggtttt ctcacaacaa ggccgggttc ctcgacaaat cttgcaaatt
     4081 ccctgggttc ctcccttctt tcgctgcttt ttaaaagagc tttttcatag gcgctgaaat
     4141 agtatttgac aagagtgtcc cacattgcaa tacgcgatat ttcatgcgcc ttcagcctgg
     4201 ctttgtttat ttcatcatca ctgagattaa tgaacatctg catgaattcc atgatgtcgc
     4261 tgaccacctg gtcttcattg ttgtccgtcc tttcaatcac tgcaatgccg tttcccggat
     4321 ccttgaagta ttccctgacc cacaggccga aacctgacag ggatgttgtg atggtaggta
     4381 tggaaaacat aaggctttca aggggtgtat atccccatgg ctcgtaatag gacgggaagg
     4441 ccgaaagatc gaatcctatc agaaggtcat agtatggcag gttgaatatc ccgtcatttc
     4501 cattcagata ggaaggtgcg aatatgattt ttacctgtga ctccttattg ttgtccagct
     4561 ggtttgcatt tattctctga attaccggat ccgtggccgg ataatgcaga tagtgcgtga
     4621 ggtacctgtc gccgccgtgt tcctcggcgt cgttgtacag tatctccata agatcctgcc
     4681 tcggtcccat gtgataggca ggcatcatta tgaaggcaac acattccctt ttcggcttct
     4741 cttttttatt cagcctgcca agtgcgtcaa taaatatgtc aagacctttg ttacggaatt
     4801 catagcgtcc gctgttggca ataaaaacgg cgtcgtcaga gatgccgtaa cccagtactg
     4861 cttctgcaac agccttgagt ttcagtctgg cagtatttct tctttcagca aaatgttttt
     4921 cgtccggaac gaaggaatcc tcaaagccat tgggagtcac aatatccaca tccttgccca
     4981 ggaaatggct gcattccttt gaggttatcc cgctgacagt ggtaaaaaca tcagcctctg
     5041 ccgcggttat cctttcgagg gactgtttgg cggcaacatt gaattcacgg gcaatctgcc
     5101 ccgggttata ttccttcatt ttgccataaa gcggccggtt gtttcctgct atgctccgtc
     5161 caagcacggt tgcatgcgta gtgaaggcag tagccaccca gggagcattg tgttcgaggt
     5221 agagcacacc cgttcccgtc atccattcat ggaactgggc gattatatcg tgatgctcct
     5281 ggtaaaatga agtgaagctt tcgatgacct ttgccgcggc atagccgaac agcgccggtt
     5341 caatatagtc ccactggccg gtaatgctgt caagcttgta tgtctcccag aatcttgcaa
     5401 atatttcgtt ctgctgtccg aaatagggag tgaagtctat aaggaacact attggctttc
     5461 cggctatgtt ccatcttccg gtcttgattc tcaggtcttc ggacagggct ttccccttcc
     5521 attcttcaaa aagggaatca tcctgcagga actcagggtt ttcagattct tccctccata
     5581 catcgggacc gatcagtatg taattatctc ccagttcttc caggatgtta agggcttttg
     5641 tggaaattac cgtatgtatt ccccccactt tattacatac ttcccagctc gtttcaaata
     5701 aaaaattaat tttcatgcta ttggcagtta tttatattgt gttaatctgt tttcttgcgt
     5761 ccgctcccgg ctgctttaac ggtttttttc cctgcaggtt tcttttctgc agtttttgcc
     5821 gtgcttttgc ggcttccggt ttttgtcccg ctgccggttc cggtctttga tgccgagctg
     5881 gttttgagca tcttggccag atcttccgct gatctcctgg gtgtgctttc aggaagatcc
     5941 gattcaatct ccgcgcttat cttctttctt gaaagattat tgatttctgc agcctgcctt
     6001 tcaattatcc tgtctttttc atgcagcaga ttgtccagtc tcgatatttc tatctgttcg
     6061 tgtgtatccg gcaggcatct gttgactcgt atttcaaaat cgctcagaac gttcatgtaa
     6121 ttcatgaatg catcatatgg cgtttcatag ggattgaaat atgcatgcac cgcaccatca
     6181 gagaaaaact tagttgccat ataatagaaa tggtcacttg cctgaaggta ttcccaatcc
     6241 cggttcatgt ggggatcatt gcatcttttg accttgtccg acaactggta aagcttatcg
     6301 agggctgcta cctgcagttc attgcctgtc caggcagtca ggtcgcgttc ctcatcggcc
     6361 caggaaatgg gggaaggcac gcttatacct gcaacaggct gaagctcgtc agcaatttcc
     6421 gagggagtca tgaacttgaa cggtgttttc ttcagtatca ttcccggaag gttttccagg
     6481 aagtcgaaaa tgccggtttc cttccagtta tgttccccga aggtttcata atcgaggaat
     6541 atattgagca gttcactttt gggtgcaagc ttgttgatcc aggaggcata tttctctgct
     6601 gtcagcggcc aggcgctcca tgacctgttg gaaaacctga aagcaatatc gtcactcatt
     6661 acaaagttcc tcagtaaaac cttgagcctc ggattgatag tattacagta caggaagttg
     6721 gggctcttcc atcccaggat gtgtttcgca ccctcggtga gtatggcctt gaatcccata
     6781 tctgccaccc atgaaccgat ctggtcggag tagatcagtt ccgtattcct gaaggattgc
     6841 gtttcaacac cgagatgggt tctcatcagt tccctgtgca gttcaacctg ctgctcaaac
     6901 tggattttgc ttttcaggga agagagggaa tgggaatcgg tttccgacag gaattccacc
     6961 atgcctgtgt cggcaagctc ccggaatgat tcgaggactt ccggtgcata cagcctgaac
     7021 tggtttataa ccattccggt aagtgaaaag gtgaccttga atttaccctt atgcctgcgg
     7081 attaggtcca gaattatcct gtttgcaggc agatagcact tgtctgcaac ctttcttagt
     7141 attgattcat tggtaaaatc atcataataa taatgatcat gccccagatc gaaaaatctg
     7201 tatctcttca gcctgaatgg ctgatgtacc tgaaaataaa agcatattgc ctttggtcct
     7261 gctgtactca tatcctgata ttattttttc caaatgactc ttt
//
LOCUS       DAOSPX010000160         7232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9620_length_7232_cov_4.685523, whole genome shotgun sequence.
ACCESSION   DAOSPX010000160 DAOSPX010000000
VERSION     DAOSPX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9620_length_7232_cov_4.685523"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLZ75_08635"
     CDS             <1..538
                     /locus_tag="PLZ75_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS72497.1"
                     /translation="NDPVTAYNGELRIWKNGTELGHWGPGFPNGHWLKDKWYNNPADP
                     PFEGFRWRTDENLNINWIWIEFWHNSDGPSSHIKFSNIVMAREYIGPIYDPATALPEI
                     PGKSKNITVSPNPGSGTINISNIEKICKTSVFNVLGKKVMETESDTEIDVSYLNPGIY
                     FLNIETENDQYRVRIVKN"
     gene            672..2144
                     /locus_tag="PLZ75_08640"
     CDS             672..2144
                     /locus_tag="PLZ75_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72498.1"
                     /translation="MRINYLSIILLAAGFIFLASCYRLPDRFSAAEELPDIFPDIAGV
                     SIPVNIAPLNFRLDSKPRKTLVTLEGRNSIMKTGGKNKIRFRVKKWHKFLEQNKGGEI
                     LFTVYTKNATGWERHLPFSIFVKELPADPYLVYRRIAPGYESWSRMGIYQRNISNFDE
                     DVVLDNRMLTGNCMNCHSFSNHNPEMMMLHLRGKIAGTLIARDGDVIKIDTRTKETKY
                     NCVYPYWHPSGRYIAFSVNNISQVFHSVKEKRIEVMDSESDLVVYDINSNVLIRSRLV
                     SSEDSFETFPAFSADGRELYFCSARKENMPSGYDNVRYSLCKTDFDPDSGTFGRKTDT
                     LISEASTGKSISFPRASPDGKYLMFTMSDYGNFSIWHKEADLYLLDLSSGSYHPLDAA
                     NSGDTESYHSWSSGSRWFVFSSRRIDGLYTRLYLSFLNEDGTATKPFLLPQKDPDYYD
                     YSLYSFNVPEFVRGRVRYKGRSMINAIGSEPKSVIFEAKD"
     gene            2151..3317
                     /locus_tag="PLZ75_08645"
     CDS             2151..3317
                     /locus_tag="PLZ75_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72499.1"
                     /translation="MAKTYYALLPSVTGSVEEEFLQILDQILDISNSGFTPVRINIFA
                     DLPDFASLITLRGKITEICTHDLGPACPAVSLTAQPPEKPWKTAVEAVFIKTGSASVR
                     GGYHNTIPYIVLESSSGRELLAGGLSSYMHPDDTRAAAEAAFDTVLRVLEKEKMSADN
                     IVRQWNYIGNILQMKNDFQNYQIFNEVRSEYYGRYRSLSRYPAATGVGMNHGGVIIDI
                     CAFQPSDFVSLVAVKNPGQVNAYEYGQKVLKGSAGKGKNIKNPPQFERGLLVSAGDHS
                     VLLISGTASIIGQETVGTGDIDKQTTVTIENISKLSDPSRIGQLMGYPGEPVKKYGFL
                     RVYVKRQNDFSTVRQKCKEYFPGVPVSYIGADICRDDLLVEIEGEAELMFNKKH"
     gene            3417..5774
                     /locus_tag="PLZ75_08650"
     CDS             3417..5774
                     /locus_tag="PLZ75_08650"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOS72500.1"
                     /translation="MNLQRITLRGSLHFPGIFLLLSASLLLTAVPASSQKRKSSRSAT
                     PAAVQDILLAEKGTSQYRIIIPASPVPDERRAAEVLQDYFLQVTGAALPVIAADKNEK
                     GVPFEIILGQNERLDRLGIQINFNELGEDGFIIRTDSLRLIIAGGSKKGTLYGVYTFL
                     EKYLDCRMYSPAVRIVPKKEKILLGRIEDRQIPLIKYRTIHYRGTWDQEYADWHKLSH
                     DARGERTAWGLWVHTFNHLVPPDIYYKDHQEYYALRNGRRIPTQLCLTNPDVLRITVQ
                     NLRKEMAKNPEALYWSVSQNDNTAYCMCEHCQALDRQEGSPSGSMISFVNSVAEHFPE
                     KIISTLAYQYTRQAPVNLRPLDNVNIMLCSIEMRRDIPFAAATDPVSRAFVKDVEDWN
                     KIAKDIIVWDYVIQFSNLISPFPNLHVLQPNLKFFVENGVNSMFEQGNREVGGEFSEL
                     RSYMLSKLLWDPYTDTDALMDDFLNGYYGAAGLPIRNYIDEMRAAVLDAGQPLSIFGS
                     PNSAAGTYLTPSLIDRYKELFDRAESLVADSTVLLERVRIARLPLNFAIMEQAKKNFS
                     GEGGVFIRSGEGWTVRPEIRSMVDPFVDLCIRQGVTRIKEWNVTPEAYRSAMYRLFFQ
                     GRNEHLAYGKPVRFLSPDISAIPEAKRGMLTDGIRGSHDIEYNWLDFRGSNLDAVIDL
                     GEIKRIQHIECAFYQLAAWLSIIPDRVDFLVSADGEKFENVGTVPNTLPIDQYDSYQR
                     DFMVDFKPRDARYVRVVAHTIGNTPEWHPGSGQPARMHIDEIVVD"
     gene            5858..7204
                     /locus_tag="PLZ75_08655"
     CDS             5858..7204
                     /locus_tag="PLZ75_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS72501.1"
                     /translation="MKRRDFVNRVILTGGALSLGIGCKSKVRTGTGTEKSMPVSTDTA
                     SGRTIREPEREIPVLAETDVLVIGGGPAGTAAAIAASRTGAETYLVERYNHLGGLWTG
                     GLVLPLLSTHALDRHQRQRKVIFGIGEEMSGRLFDLGMAIHEVNPVIDPEAGKFVLDE
                     MIREAGVRMLYHSWASNVIMDGNVIRGVFIETKSGRMAVLAKVVIDCTGDGDIFHLAG
                     DDYDTMNYAIGLVHRLGNVDRIDPDKPGYEKKNLGDPTPLKGVNWVNMWGRENQDALD
                     VVNLSNLQMDYRREIWNSVQELRKTPGHEEVFLLDTASQLGVRMSRILDGEYRLRLED
                     TMTYRSFSDAIGISGAWTTMLYRGRKIPASERPWWQIPYRSLVPRKTENLLVAGRCFC
                     FEKELVEDTRIIGTCLVTGQGAGAAAGLAVREKVFPRNVNVKRLRDLLKQQGVWMG"
BASE COUNT         2017 a         1521 c         1860 g         1834 t
ORIGIN      
        1 taatgatcct gtaacggcat ataacggaga actaaggatc tggaaaaacg gaactgaact
       61 gggacactgg gggccgggtt ttccaaacgg tcactggctc aaggataaat ggtataacaa
      121 tcccgctgat cctccttttg aggggttcag gtggcggact gatgaaaatc tgaatatcaa
      181 ctggatctgg atagaattct ggcataattc ggacggtcct tcgagtcata tcaaattttc
      241 aaatattgta atggccaggg aatatatcgg accaatttat gatcctgcca ctgctttgcc
      301 tgaaataccg ggcaaaagca aaaatatcac agtttcaccc aatccgggta gcgggacaat
      361 aaatatctca aacattgaaa aaatatgcaa aacatcggta ttcaatgttt taggaaaaaa
      421 agtaatggaa actgaatcgg ataccgaaat cgatgtgagt tatttaaatc cgggaattta
      481 ttttctgaac attgaaacgg agaatgatca gtatagggtc agaatagtaa aaaactgaaa
      541 attccccgga gcctggcaca acatgatccg cagacggaga ataataattt tccctgattc
      601 tttgtatttt taagccggag agaggaaaaa tctatccaat gattacggaa tttatattaa
      661 accgactggc tgtgagaata aattatctgt caatcatttt gctggctgcc ggatttattt
      721 tcctggcttc atgctacaga cttcctgacc gcttttcggc ggcagaggag ctgcctgata
      781 tattccccga cattgccggt gtaagtattc cggtgaacat agcacccctg aatttcagac
      841 ttgatagcaa accacggaaa actcttgtca ccctggaggg tagaaacagc atcatgaaaa
      901 ccggggggaa gaacaaaatc cgtttccggg tgaaaaaatg gcataagttc cttgagcaaa
      961 acaagggtgg tgaaatactt tttacggtat atacaaaaaa tgctactgga tgggagagac
     1021 atctgccttt cagtattttt gtaaaagagc tgcccgcgga tccctatctt gtttaccgcc
     1081 gtattgcacc gggatatgaa tcatggagca gaatgggcat ttatcagcgt aacatatcca
     1141 attttgatga agatgttgtt cttgacaaca ggatgctaac gggaaactgt atgaactgtc
     1201 actccttcag caatcacaat ccggaaatga tgatgctcca cctcagggga aagattgccg
     1261 gaaccctgat agcccgcgac ggtgatgtaa ttaagattga cacaagaaca aaagagacaa
     1321 agtataactg tgtttatcct tactggcatc cttcaggcag atatattgcc ttctcggtca
     1381 ataatatttc acaggttttc cattcggtga aggaaaaaag gatagaagtc atggattcgg
     1441 aatcggatct tgttgtttat gacatcaatt caaatgtact gatcagaagc aggcttgttt
     1501 cgtcggaaga tagttttgaa acttttccgg cattttcagc cgacggcagg gagctctact
     1561 tctgttctgc caggaaggaa aacatgcctt caggatatga taatgtcagg tacagcctgt
     1621 gcaaaacgga ctttgaccct gattcgggaa cttttggcag gaaaactgac acacttatca
     1681 gtgaagcttc aacagggaag agcatctctt ttccgagggc ttccccggac gggaaatacc
     1741 tgatgtttac catgtcggat tacggaaact tttcaatatg gcacaaggaa gccgatcttt
     1801 atctcctgga tctgtccagc ggcagctatc atccccttga tgcagcaaac agcggggata
     1861 ctgaaagcta tcattcatgg tcgtcgggat cacgctggtt tgtattcagc agccggagaa
     1921 ttgacggtct ttacacaaga ctttatttat ccttccttaa tgaagacgga acagccacaa
     1981 agccttttct gcttccacag aaagatcctg attattacga ttacagcctg tactcgttca
     2041 atgtgcccga gtttgtcagg ggcagggtga ggtataaggg gcgcagcatg ataaatgcca
     2101 ttggttcgga gccaaaaagt gttatttttg aggccaaaga ctaacctgaa atggctaaaa
     2161 cctactatgc gctccttcct tcggttacgg gcagtgtgga agaggaattc ctccaaatcc
     2221 tggatcagat actggatatt tcaaattccg gctttacccc ggtcagaata aacatttttg
     2281 cagatctgcc tgattttgct tcattaatta ctttaagggg gaaaataacc gaaatatgca
     2341 cacacgatct gggtccggcc tgtcctgctg tttccctgac ggcacagcct cctgagaaac
     2401 catggaagac ggctgttgag gctgtgttta taaaaaccgg ttccgcttct gtccggggcg
     2461 gttatcataa tactatcccg tatatagttc ttgagtcttc ctccggcagg gaactgctgg
     2521 ccgggggact gagcagctat atgcaccctg atgatacgag ggccgctgcg gaggcggcat
     2581 ttgacactgt tctccgggta cttgaaaagg aaaaaatgtc ggctgacaat attgtgcggc
     2641 agtggaatta tatcggaaat atccttcaga tgaagaatga ttttcaaaac tatcagatat
     2701 tcaatgaggt aagaagtgag tactacgggc ggtaccggag cctgagccgg tatccggccg
     2761 ctacgggtgt gggtatgaac catggaggag tgatcataga tatctgtgct ttccagccgt
     2821 cagattttgt ttccctggtt gccgttaaaa atcccggtca ggttaatgca tatgaatacg
     2881 gccagaaggt tctgaaggga tctgccggca agggaaaaaa tatcaaaaat cccccgcagt
     2941 ttgagcgagg actgcttgtt tcggccgggg atcattcagt tttactgata tcaggcaccg
     3001 cctccatcat cgggcaggaa accgtcggaa caggggatat tgataaacag acaactgtta
     3061 cgattgagaa tatcagcaaa ctttccgatc cttccagaat cgggcagctg atgggatatc
     3121 ccggggaacc tgtcaaaaaa tatggatttt taagagtcta tgttaagagg caaaacgatt
     3181 tcagcactgt tcgtcagaaa tgtaaagaat attttcccgg agtacctgtt tcatacattg
     3241 gagcggatat atgccgtgat gatctcctgg ttgaaattga aggggaagca gaattgatgt
     3301 ttaacaaaaa acactagccg gggccggcag acaggagttc agatttccgt cggactgttg
     3361 ccctgacatg gtgcccgtag ttacaatgaa taattatcca gtaagataaa tatattatga
     3421 atttacaaag aatcacattg cggggatcac ttcatttccc cggtatattc ctgctattgt
     3481 cggcatcact gctgttgacc gcggtaccgg cttcatcgca gaagaggaaa agcagcaggt
     3541 ccgctacccc ggctgctgtt caggatattc tgctggcgga gaagggtaca agccaatacc
     3601 gtattatcat tcctgccagt cctgtacctg atgagcgcag agccgcggaa gtacttcagg
     3661 attattttct tcaggtaaca ggtgcagcac tgcccgtaat tgccgctgac aaaaacgaga
     3721 agggagttcc gtttgagata atcctgggac aaaatgaacg cctggacagg ctcggtattc
     3781 agatcaattt caatgaactc ggcgaagacg ggtttataat cagaaccgac agtctgagac
     3841 ttattatagc cggaggcagc aaaaagggga cattgtatgg tgtttatact tttctcgaga
     3901 agtatcttga ctgccggatg tattcacctg cagtcaggat cgttccgaaa aaggaaaaga
     3961 tacttcttgg cagaatagag gacaggcaga taccgttaat aaaatacagg accattcatt
     4021 acaggggtac ctgggatcag gagtatgccg actggcacaa gttaagtcat gatgcacgcg
     4081 gagaaaggac tgcatggggt ttgtgggttc atacctttaa tcatcttgtt cctccggata
     4141 tttactataa ggatcatcag gagtattatg cactgagaaa cggcaggagg ataccgaccc
     4201 agctctgcct tacaaatcct gatgtgctca ggatcacggt gcagaacctc aggaaagaga
     4261 tggcaaaaaa ccctgaggca ctttactggt ctgtgagcca gaatgacaat acagcctatt
     4321 gcatgtgtga gcactgccag gctcttgaca gacaggaggg atccccttca ggatcaatga
     4381 tctcatttgt aaacagtgtg gcagagcatt tcccggaaaa gatcatttcc accctggcat
     4441 accagtatac caggcaggca cctgttaatc tccggcccct cgacaatgtc aacatcatgc
     4501 tctgcagcat tgaaatgcgc cgcgacatac cgtttgccgc cgcaacggat ccggtcagcc
     4561 gggcattcgt taaggatgtt gaagactgga ataaaatagc aaaggatata atagtgtggg
     4621 attacgtaat tcagttcagc aatctgatca gccctttccc caatctgcac gtattgcaac
     4681 ccaatctgaa gttttttgtg gaaaatggcg tgaactcaat gtttgaacag ggcaaccgtg
     4741 aagtgggagg agagttttcc gaactgcggt catatatgct gtcaaaactg ttatgggatc
     4801 cttatactga tactgatgcc ctgatggatg atttcctgaa tggttactat ggtgctgccg
     4861 gcctacccat aaggaattat attgatgaaa tgagggcagc tgttctggat gccggtcagc
     4921 ctctgagtat tttcggatcg ccgaattcgg cagccgggac ctatctcaca ccgtcactga
     4981 ttgacaggta caaggagttg tttgaccggg cggaaagtct tgttgcggat tcaaccgttc
     5041 tgcttgaaag agtcagaatt gcccgtctgc cgctgaattt tgcgataatg gagcaggcca
     5101 agaaaaattt ttccggggaa ggaggtgtgt ttatcagatc cggagagggc tggactgtca
     5161 ggcctgagat aagatccatg gtggatccct ttgttgatct gtgcatccgc cagggtgtca
     5221 cgcggataaa ggaatggaat gtaactcccg aagcctaccg ttcagccatg taccgtcttt
     5281 ttttccaggg caggaatgaa caccttgcat acggaaagcc ggtcaggttc ctgagcccgg
     5341 atatctctgc catacctgag gctaagagag gaatgcttac tgacggaatc aggggaagcc
     5401 atgatattga atacaactgg ctcgatttca ggggaagcaa tctggatgcc gttatagatc
     5461 tgggtgaaat aaaaaggatt cagcatattg agtgtgcatt ttatcagctt gcggcctggc
     5521 tcagtattat tccggacagg gttgatttcc tggtttcagc tgatggagag aaatttgaga
     5581 atgtcggaac tgtgcccaat accctgccta ttgatcagta tgattcatac cagcgggatt
     5641 ttatggtgga ttttaagccg cgtgatgcca gatatgtaag agttgtcgcc cacacaatag
     5701 gaaatacgcc cgaatggcat ccgggatcgg gtcagccggc caggatgcat atcgacgaga
     5761 ttgttgtgga ttgaggccat ccgttctgaa taaaagggaa attcctttca gtacgaactt
     5821 aaaccttttg acctgaataa cagataaatc caattatatg aaacgcagag attttgtaaa
     5881 cagagtaata ctgaccggtg gtgccctcag tctgggtata ggatgcaaaa gcaaagtccg
     5941 taccggtacc ggtacagaaa agagcatgcc ggtatcaacg gatacagcct ccggcaggac
     6001 gatccgggag cctgaaaggg aaataccggt gcttgccgaa actgatgtgc tggtcattgg
     6061 cggcggaccg gcaggaaccg cggctgcaat agctgccagc cgtaccgggg ctgagactta
     6121 tctggttgaa cgttacaacc atcttggcgg gctctggacc ggcgggcttg tattgccgct
     6181 tctttcgacc catgcccttg acaggcatca aagacagaga aaggtaattt tcggtatcgg
     6241 ggaggagatg tccggaagat tatttgatct gggaatggca atacatgaag tgaatcccgt
     6301 tattgacccc gaagccggca aatttgttct tgatgaaatg atacgggagg ccggagtcag
     6361 gatgctctac cacagctggg cttcaaatgt tataatggac gggaatgtaa tcaggggagt
     6421 gttcattgaa accaaatccg gacggatggc tgtcctggct aaggttgtta ttgactgtac
     6481 tggtgacggg gatatcttcc atctggccgg agatgattac gataccatga attatgctat
     6541 aggacttgtt caccggcttg gcaatgttga ccgaatcgat cctgacaagc ccggatatga
     6601 aaagaaaaat ctcggggatc ccactccgct taagggagtg aactgggtaa acatgtgggg
     6661 acgggaaaat caggatgccc ttgacgtggt aaatctttcg aatcttcaga tggactaccg
     6721 ccgcgagata tggaacagtg tccaggaact caggaaaaca ccggggcatg aagaggtttt
     6781 tcttctggat actgcatcgc agctgggcgt aaggatgtcc agaatacttg acggcgaata
     6841 caggctcagg cttgaggata caatgacata cagatcgttc agtgatgcga ttggaataag
     6901 cggggcatgg accaccatgc tttacagggg aagaaaaatc cctgcatcag aacgtccgtg
     6961 gtggcagata ccttaccgtt cacttgttcc aaggaagact gagaaccttc ttgttgcagg
     7021 ccgctgtttc tgttttgaaa aagaactggt ggaagataca agaattatag gtacctgtct
     7081 ggttaccgga cagggtgccg gtgcggctgc cgggcttgct gtcagggaga aggtgtttcc
     7141 aagaaacgtg aatgtgaaac ggctcaggga tctgctgaaa cagcagggtg tctggatggg
     7201 atgaaaaaga cggtcactgc atcggatata cg
//
LOCUS       DAOSPX010000161         7230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9623_length_7230_cov_3.949129, whole genome shotgun sequence.
ACCESSION   DAOSPX010000161 DAOSPX010000000
VERSION     DAOSPX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9623_length_7230_cov_3.949129"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="PLZ75_08660"
     CDS             complement(<1..1132)
                     /locus_tag="PLZ75_08660"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS72502.1"
                     /translation="MKRTIIITAIVVFVVMIGLMIFNRLVSKQHEVSDFTVVKKGLFE
                     IAISNAGELFAENSFDIMGPEIQESQPQRGGGGRGGQHSMRITTYKIQDLIPEGTIVK
                     KGDYVAQLDRTDYTNTLRDQLEQLTTLQANLDMKILDTAVTLTNLRDEIKNQVYVVEE
                     ARIILAESKYEPPATIRQAEIRLNKAERALEQLEKSYDLRYAQSLTDIRKTKQDLVNG
                     QELVNALQEFLSKFTITAPSDGIIIYKQEWNGTKRKTGSMINPFDRVVATLPDLSSML
                     SKTYVSEIEINKVAKGQRVVVTVDALPGKAYTGTVMSVANVGEVLPNSDAKMFEVLIA
                     IDNSGNSLRPAMTTWNRIITKTIDDAVYIPTECVYTGADSIPF"
     gene            1252..3915
                     /locus_tag="PLZ75_08665"
     CDS             1252..3915
                     /locus_tag="PLZ75_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOS72503.1"
                     /translation="MSRNYLILLPLVMIILSGSCSRTKKSLAVSDLRCENLTGPLGLG
                     TDKPRLSWKIISGENGTVQKAFQILAASSIALLDEENADLWNSGKIMSPESHLVPYRG
                     REAGSRTLCYWKVRIWDAGDRCSEWSDAAFFSTGLLGESDWKAEYIGFPHSEGYTVSP
                     QLKRTFTLDDTGEKVFLYVNSLGYHEVYVNGAKAGDYVLTPSVSQFSKRSLVLTYDIS
                     HMLRQGRNDLVIWTGRGWYSEGLPGVSASVPLVRAQAEKLSGGRWEQILVTDTLWKGR
                     NSGYSTIGTWRPHQFGGEYADASLLLPDLSAESLDRVSWTSVFSAEKPAGEATSQLCE
                     PDRIFESIHAASVVKLNDSSWLADMGKSITGYAEIRFPQLRKGSRILMEYCDHLDDEG
                     NIVNQGQRDIFVSAGAGDECFRNRFNYHGFRYIRISGLPREPLPSGIKAHLIHTGYGQ
                     ASTFECSDPDLNRIHDMISHTLRCLSLGGYLVDCPSVERLGYGGDGNASTETAQIMFP
                     LAPLYSNWLQAWADCIREDGGMPHTAPNPYNAGGGPYWCGFIITASWRTYMNYGDELI
                     LSEYYPVMQKWLGYVEKYSPRGLLERWPDTDYRNWYLGDWAVPEGVDQTDPASVNLVN
                     NCFIAVCYETMEKIAAVLGKTHDSETYRLKRRSLKNLIHNTFYDAAKSVYGSASQIDL
                     TYPLLAGIVPDSLAGTVRENLRDVILNRYGGHFACGLVGIPVFTEWAVKNREADLFRS
                     MLLKRDYPGYLYMLGNGATTTWEHWNGARSRIHNCYNGIGRWFYQAVGGIQNDETECA
                     YRSVIIDPQIPSGISWANTSQETPYGTVRVNWKMTDAGFHMEVGIPYGSRARVMIPGH
                     AEKYRMNGKEIGNETSVAEIPGGTYIFEWQK"
     gene            3903..5012
                     /locus_tag="PLZ75_08670"
     CDS             3903..5012
                     /locus_tag="PLZ75_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOS72504.1"
                     /translation="MAKINRQTVSEERDFELVRKLNPARIIIPVLIGLAVVAWLISRE
                     ISREMLSYLQLTWQSVFWLFIAICFMIGRDLGYMIRIRVLSEKDLTWRQAFRVIMLWE
                     FTSAITPSTVGGTAVAVVFIHKEDISVGRSTSIVLATSFLDELYFVIMFPLILLIVGK
                     DVLFTTSLQGTGPALLNNLVIVAVAGYSIILAWVVLVGYGLFADPEKIKKILVWIFRL
                     PVLRKWKASAEKAGDDIISSSGELKRKSVFFWIKAFTATFLSWTSRYFVINAILVAFF
                     EIRDHLLIFARQLVTWIMMIISPTPGGSGFAEVILGRYITDVIPAGPEHTVGLALAIT
                     IIWRIISYYPYLIIGSIILPGWIQRKFVKHSLKKK"
     gene            5059..>7230
                     /locus_tag="PLZ75_08675"
     CDS             5059..>7230
                     /locus_tag="PLZ75_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS72505.1"
                     /translation="MSEEILKALMELFALIVKQDGGMLASEREYVKNFLVRQLSSENV
                     SQYLALFDEHAGPVNDSEDSKDTNVKPSVRDSVKILAICKKINRTLNGEQKVITLMRL
                     FELVNADRRFTPQRMNIINTVAEVFNIPPEEFRSVGIFVTNNETENLSDPSILLFHPG
                     AHKCRVCENMEAGYPGTLLVCLRVASVDLLFMKYISGEQLKLNGLPIFSGNIYIFPKG
                     GSLRSAQGYPIYYSDISSRFLSETIAHKISFRVEHLSYFFVEGHPAVDDVSFSAEQGQ
                     LIGIMGGSGSGKTTLLNLVSGIVKPSAGSVRVNGLDINSDTFPEGVLGYVPQDDLLIE
                     DLTVFENLYYAACLCFKNKTKAEIISQVEQTLLNLGLFEKRNLKVGSPHNKVISGGQR
                     KRLNIALEMIREPSVLLLDEPTSGLSSRDSENLMDLLRELALKGKLVISVIHQPSSEI
                     FKMFDNILIMDQRGSMAYYGNPVDAVIYFKTLDAQINASVGECPSCGNINPETIFSII
                     ETQVVDEFGKYTEKRKIKPSEWASLFRAKFPLPPEAEETEKPPSNLDRPSVLQQFRIY
                     LSRDLKSKLANRQYLLLTLAVAPLLGLILSFIIRYIPDPDSDVYLFAENENIPIYIFM
                     GIIVALFLGLTISAEEIFRDRNILRRERFLNLNRSSYLLSKVSVMLIISAIQTFLFCA
                     VANPVLGIRGLFMYYWIALFSTSFCANMLGLNISSAFNSVIT"
BASE COUNT         1913 a         1759 c         1763 g         1795 t
ORIGIN      
        1 caaagggaat actgtcagct cccgtgtaaa cacattcagt cggtatataa acggcatcat
       61 cgatagtttt ggtgattatc ctgttccagg ttgtcattgc cggcctgagt gaattaccgc
      121 tgttatctat tgcgataagc acctcaaaca tttttgcatc ggaattagga agcacctccc
      181 ccacatttgc cacactcatc acagtgccgg tatatgcttt ccccggcaat gcatcgacgg
      241 ttaccactac cctctgccct ttggcaactt tgtttatctc aatctcactt acatatgtct
      301 ttgacagcat tgaggagaga tccggaaggg tggcaacaac acggtcaaag ggatttatca
      361 ttgaccctgt ctttctcttt gttccgttcc attcctgctt gtatattatt attccgtcag
      421 agggagcggt tatcgtaaac tttgaaagaa actcctggag agcattcaca agttcctgtc
      481 cgtttacaag gtcctgtttt gtctttctga tgtcggtaag tgactgtgca tacctgagat
      541 catagctttt ttccagctgc tccagcgcac gttctgcctt gttaagcctt atttcagcct
      601 gacggattgt tgccggaggc tcatatttcg attcagccag gattattctg gcttcctcaa
      661 caacataaac ctggtttttt atttcatccc tgagattggt aagggtaaca gcagtatcca
      721 gaatcttcat gtccaggttg gcctgcagag tggtcagttg ctcaagctga tcccggagag
      781 tattggtata atcagtcctg tcaagctggg ctacataatc gcctttttta acaatggtgc
      841 cctcaggtat caggtcctgt atcttgtagg tggtgatacg catgctgtgc tgaccgcctc
      901 ttccgccacc acccctctgg ggctggctct cctggatttc cggacccatt atatcgaagg
      961 aattctcggc aaaaagctca cccgcgtttg atattgctat ttcaaacagc cccttcttaa
     1021 ccactgtgaa atcacttact tcatgttgtt tggataccag ccggttaaaa atcatcagcc
     1081 ctatcatcac aacaaaaaca acaatagcag ttatgattat ggttcttttc atttcaaatt
     1141 atacttttgt acgaccatta ttcactatat agaccattat aaaggcaatt tattccaatt
     1201 ttcaaaaata aaatgacaaa cggaataaaa ttagtattta atattcacag aatgtcacga
     1261 aactatttaa tcctgctgcc acttgtaatg atcattctca gcggaagctg ttcacggact
     1321 aaaaagtccc tggccgtttc ggatcttcgc tgtgagaatc tgaccggtcc cctgggactg
     1381 ggaacagaca agccacgcct gagctggaaa ataatatccg gggagaatgg cactgttcag
     1441 aaggcttttc agatacttgc tgccagcagt atcgcattgc tcgacgaaga gaatgccgat
     1501 ctctggaatt ccggcaagat aatgtctccg gaaagccacc ttgttccgta ccggggcagg
     1561 gaagccggat caagaaccct ttgctactgg aaagtgagga tatgggatgc cggtgacagg
     1621 tgctcggagt ggagcgatgc ggcatttttc agcacgggac tgctgggaga atcagactgg
     1681 aaggcagagt acataggctt tccgcattca gagggataca ctgtcagtcc ccagctgaaa
     1741 agaaccttca cccttgatga taccggggaa aaagtctttc tctatgtcaa ttccctcgga
     1801 tatcacgagg tgtatgtcaa cggagcaaag gcaggtgatt atgtccttac tccctcggtt
     1861 tcacaattca gcaaaagatc ccttgttctt acatacgata tcagccatat gctcaggcag
     1921 ggcagaaatg acctggtgat ctggaccggc cggggctggt acagcgaagg acttcccgga
     1981 gtatccgctt ccgttcccct tgtgagggcc caggccgaga aactcagcgg gggcaggtgg
     2041 gaacagatcc tggttactga tacactctgg aagggacgaa acagcggata ctccaccatt
     2101 ggaacatggc gtccgcacca gttcggaggg gaatatgccg atgcatcgct cctgctgccg
     2161 gatctcagcg cggaatccct cgacagggtc agctggacat ccgtgttttc agctgaaaag
     2221 cccgccggag aagcaacatc ccagctttgc gagcccgaca ggatctttga atccatccat
     2281 gccgcctcgg ttgttaaact gaatgacagt tcctggcttg ccgacatggg aaaaagcatc
     2341 accggctatg ccgagatccg tttcccgcag ttgcgaaaag gcagcaggat cctgatggaa
     2401 tattgtgatc accttgatga tgaagggaat atagtcaatc agggacagag ggatattttt
     2461 gtatccgccg gtgccggtga tgaatgcttc cggaacaggt tcaactatca cggattccgc
     2521 tatatcagga tttccggtct gccgcgggag cccttgccgt ccggtataaa ggctcacctg
     2581 atacacacgg gatacggaca ggcttcaacg tttgagtgtt ccgaccctga cctcaacagg
     2641 atacatgaca tgatcagcca cactctcagg tgcctcagcc tgggaggata cctggtcgac
     2701 tgcccctcgg tggaaaggct tggatacggt ggtgacggaa acgcatccac cgagacagcc
     2761 cagataatgt tccctcttgc accactctat tcaaactggc tgcaggcatg ggccgactgc
     2821 atacgtgaag acgggggaat gccgcatacc gcacccaatc cctacaacgc cgggggaggt
     2881 ccctactggt gcggattcat catcaccgcc tcatggagaa catacatgaa ctacggtgat
     2941 gaattaattc tctcggaata ttacccggtg atgcagaaat ggctcggata cgtggaaaag
     3001 tattccccgc gcggcctact tgaacgatgg cccgatacgg attacaggaa ctggtacctg
     3061 ggtgactggg ccgttccgga aggagttgac cagaccgatc ccgcttcagt aaacctggtc
     3121 aataactgtt ttatagccgt atgctatgaa acaatggaaa aaattgccgc cgtactggga
     3181 aaaacacatg actcggaaac ataccgcctg aaacgccggt ccctgaaaaa ccttattcac
     3241 aacacatttt atgatgccgc aaaatcagtt tacggaagcg cttcccagat tgatctcacg
     3301 tatcccctgc ttgccggtat cgttcctgac agccttgccg gaacagtaag ggaaaacctg
     3361 agggatgtga tactaaacag gtacggaggc cattttgcat gcgggcttgt cggaataccg
     3421 gttttcaccg agtgggcggt aaaaaacagg gaggcagacc tcttccggtc catgctgctt
     3481 aaaagggact atccgggata cctgtacatg ctcggcaacg gtgccaccac gacatgggag
     3541 cactggaacg gtgccaggag caggatacat aactgctaca acggaatcgg ccggtggttt
     3601 taccaggcag tggggggaat acagaatgac gaaacggaat gtgcctacag atcggttata
     3661 atagatcccc agataccctc aggaataagc tgggcaaata catcccagga aacaccatac
     3721 ggcaccgtca gggtgaactg gaaaatgaca gatgccggct ttcatatgga agtagggatc
     3781 ccttacggaa gcagagccag ggtgatgata cccggacatg cggaaaaata caggatgaac
     3841 gggaaagaaa tcggaaatga gacttccgtg gcggaaattc cgggaggaac ctatattttt
     3901 gagtggcaaa aataaacagg cagactgtga gtgaggaaag agattttgaa ctggtaagga
     3961 aactgaatcc cgccagaata atcattcccg ttctgatcgg actggcggtg gttgcctggc
     4021 tgatatcgcg ggagatcagc cgggaaatgc tgagttatct ccagctcaca tggcagtcag
     4081 tattctggct cttcatagca atatgcttca tgataggaag ggacctcggc tatatgatca
     4141 ggatacgggt actgtcagaa aaggacctta cctggagaca ggcattcagg gtaatcatgc
     4201 tctgggaatt tacctcggca attactccgt caaccgtggg aggcactgcc gtggctgttg
     4261 ttttcatcca caaggaagac atctcagtag gccggagcac ttccattgta cttgcaactt
     4321 ccttcctgga tgaactgtat ttcgtgataa tgttcccgct catccttctt atcgtcggaa
     4381 aagatgtcct tttcaccacc tctctccagg gcaccggccc cgcccttctg aacaaccttg
     4441 tcattgtggc tgtggcagga tactcaataa tactcgcctg ggtggtcctg gtaggatacg
     4501 gtcttttcgc cgatccggaa aagatcaaga agatccttgt ctggatcttc aggctgcccg
     4561 tgctgagaaa atggaaagct tcagccgaaa aggcaggaga tgacattatt tccagctccg
     4621 gggaactgaa aaggaaatcc gtttttttct ggataaaagc atttacggca acattccttt
     4681 catggacatc caggtatttt gtgataaatg caatactggt tgcattcttt gaaataaggg
     4741 accatctgct gatctttgca cgccagctgg tcacctggat catgatgatc atcagcccca
     4801 ccccgggggg aagcggattt gccgaagtca tactggggcg ctatataacc gatgtcattc
     4861 ctgccggccc cgagcatacc gtgggcctgg cccttgcaat aacaattatc tggagaataa
     4921 taagctacta tccttattta ataatcggaa gcataatatt accgggctgg atacaaagaa
     4981 aattcgtaaa acattctctc aaaaagaaat agttttataa cttagatgcg tctgcaagat
     5041 atatagcact gagcatcgat gagtgaagaa attctgaagg ccctgatgga attatttgcc
     5101 ctgattgtca agcaggacgg gggtatgctg gcaagtgaac gggaatatgt gaaaaacttc
     5161 ctggtcaggc agctcagcag tgaaaacgtg agtcagtacc tggctctttt cgatgaacat
     5221 gccggccctg tgaatgacag tgaggactcc aaggacacaa atgtaaaacc atcagtcagg
     5281 gattccgtca agatactggc catatgcaaa aagatcaacc ggacacttaa cggggaacag
     5341 aaggttatca ccctgatgcg cctgttcgag ctggtgaatg ccgaccggcg cttcaccccg
     5401 caaagaatga atatcatcaa taccgttgcg gaggttttca atatcccccc ggaggaattc
     5461 agatcagtcg ggattttcgt tacaaacaat gaaacggaaa acctgtctga tccgtccata
     5521 ctgcttttcc atcccggtgc ccataaatgc agggtctgtg aaaacatgga ggcaggatat
     5581 cccggtacgc tgctggtatg cctgagagtg gcaagcgttg acctgctctt catgaaatat
     5641 atatccggag aacagctgaa gctgaacggc ctcccgatat tctcaggaaa tatctacatt
     5701 tttcccaagg gcggctcgct cagatctgcc cagggatacc cgatatacta cagtgatatc
     5761 agttcaagat tcctttccga aaccattgct cataaaattt ccttcagggt tgaacatctg
     5821 tcatactttt ttgtggaggg ccatccggca gttgacgatg tgagcttttc cgcggaacag
     5881 ggtcagctca tcgggattat gggaggaagc ggatcgggca agacgacact gctgaatctt
     5941 gtttcaggaa tcgttaagcc atcagcgggc tcagtaaggg taaacggact tgacatcaat
     6001 agtgacactt tccccgaggg tgtcctggga tatgtgcctc aggatgacct gctgattgag
     6061 gaccttacag tttttgaaaa cctttattat gctgcctgcc tgtgctttaa aaacaaaaca
     6121 aaagcagaaa taatttctca ggtcgaacag actcttctga atctggggct ttttgaaaaa
     6181 agaaacctga aggtgggatc tccccataac aaggttatca gcggcggcca gagaaaaagg
     6241 ctgaacatag ccctggaaat gatcagggaa ccatccgttc tgcttcttga cgaacccact
     6301 tccggactgt catcaaggga ttccgaaaac ctgatggacc tgctcaggga actggccctt
     6361 aaggggaaac tcgttatttc cgtgatacat cagccctcat cggaaatatt caagatgttc
     6421 gacaatatcc tgataatgga tcagcggggc agcatggcat actacggcaa tcccgtggat
     6481 gcagttatct atttcaagac tctcgatgca cagatcaatg catccgtcgg ggaatgtcct
     6541 tcctgcggga atataaatcc cgaaacgata ttcagtatta tcgaaacaca ggtggttgac
     6601 gagttcggga aatataccga aaagagaaag ataaaaccgt cggaatgggc atcactgttc
     6661 cgggcaaaat tccccctgcc gccggaagcg gaagaaacgg aaaaaccgcc cagcaacctt
     6721 gacagaccgt ccgtactgca gcagttcagg atttaccttt cacgcgacct gaaaagcaaa
     6781 ctggcaaacc gccagtatct tctgctcact cttgccgtgg ccccccttct cggactcatc
     6841 ctttccttta taataagata tattcccgat cccgattcag atgtttacct gttcgcggaa
     6901 aatgaaaata ttcccatcta catcttcatg ggcataattg tggcactctt tctcggactt
     6961 acgattagtg cggaggagat cttccgtgac aggaacatac tcaggaggga aaggtttctg
     7021 aacctgaacc ggagcagcta ccttctttca aaggtcagcg tgatgctgat catttcggca
     7081 atccagacct tcctgttctg tgcagtggcc aatcccgtac tgggaatcag ggggctcttc
     7141 atgtactact ggatcgccct gttcagtaca tccttctgcg ccaatatgct cggactgaat
     7201 atttcttcgg catttaactc cgtaataacc
//
LOCUS       DAOSPX010000162         7154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9767_length_7154_cov_4.070432, whole genome shotgun sequence.
ACCESSION   DAOSPX010000162 DAOSPX010000000
VERSION     DAOSPX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9767_length_7154_cov_4.070432"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PLZ75_08680"
     CDS             complement(<1..984)
                     /locus_tag="PLZ75_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72506.1"
                     /translation="MRHVLIVFLALALLMPGSGCGSHRTPERMIGIKIYDYDGDFGLL
                     AERWDKMGINTAFVSKDLAANAEFRQVLKEHEIKTFIIFPVFYDPAALKEDSSLYAVT
                     DRGRKARESWVEFVCPSRHGFRNRKIREAADLARELKPDGMSIDFIRHFVFWEMIYPD
                     RTAGSIDMACFCDSCTGAFLAGQGISLPDSCIRVGQKSEYIIDHYQDEWNDYRTGLIA
                     SMAEGIADALHRVNPDLKIMIHAVPWRDADFDGANIRVAGQDLKKLAACADYISPMCY
                     SQMLRRDAGWISSVVKEADGKAPGMIVPSIQVYPAYIDDPFSVDDFRRCVDA"
     gene            complement(1083..1826)
                     /locus_tag="PLZ75_08685"
     CDS             complement(1083..1826)
                     /locus_tag="PLZ75_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HOS72507.1"
                     /translation="MATVMLTFCFWGCGNSGVKKAPDGHTAQISLDWHGTYSGNLPCA
                     DCERIETELTINDDLTYILTSSHIKGDNEYTDTLSGRFTWKGNNIRLEGIPKNERSPW
                     FKVEEGQVRYLDMQGKEIKGELENYYILRKNGNTSVEDKRWQIVEIYGKPVEGSPETH
                     YMIFYSEDGFVSAKMNCNMLRIPYKIQNEFRVRFGHGMSTLMACPDDLEQEFLQVLSE
                     ADNLSTDGKYLSLNKARMAPLARFVVADL"
     gene            complement(1980..2903)
                     /locus_tag="PLZ75_08690"
     CDS             complement(1980..2903)
                     /locus_tag="PLZ75_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOS72508.1"
                     /translation="MKTRKTNVHKFLAYFILISFAFYAASCSSGKPERVLFIALDGIS
                     IEGYQTAKTPNIDKLMQKGSFSLKTRVVMPSVTLPNWTSHLTGSGPEQHGVTDNGWLI
                     DNHVLPAVDTDDKGYYPSIFKILKDQVPGVRTAFYYNWANLIYPYNRDYLDEVRFQEN
                     YEYIENFERAFEFIRENREKPTFVFLYTVHTDGAGHSHGWMSPEYIESIENADFHIGA
                     LLDKMEKEGMLEDTHIIVTSDHGGVNKGHGGVTPTEMEVPWSLTGPGVKKNNKITGPN
                     NTVNSAFVVAHLFKCSTPESWTGRMIHSIFE"
     gene            complement(2920..3870)
                     /locus_tag="PLZ75_08695"
     CDS             complement(2920..3870)
                     /locus_tag="PLZ75_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72509.1"
                     /translation="MRIIFKILIILPLMFSVLTSCRHKQKSRGKHEVSEDFPAEMVKF
                     TPYEHNPVFTGTKENTWDRHIRERGFILFEAGLYRMWYTGYDGGGNSVKFLGYATSPD
                     GIHWSRYEGNPVFDSKWTEDMFVFRHGSKYYMYAEGTGDVAHLLISDDGINWNEQGDL
                     IILTTRGDTLPGPYGTPSVLVEDGKWHLFYEINDEGIWHAVSDDYITWTNVSDEPVLK
                     KGPGEYDSGAVASNQIIKFRGKYYMYYHGTSDSGWAQPGAGSIWTSSVAMSEDLDNWV
                     KYPGNPIVEGDHSSPIMVYDGEKYRLYTMHDEVWLFNPAQ"
     gene            4120..5487
                     /locus_tag="PLZ75_08700"
     CDS             4120..5487
                     /locus_tag="PLZ75_08700"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS72510.1"
                     /translation="MKKPSFSRREFVRNTSLGVAGAALSAKGITSFSPVALQDTGKPA
                     LLGGTPVRPKDRILGSSWPIYDDSDIQLYLEAFKSNIWSEYSHIESERVYQTEKKYAA
                     LMGVNYCVATSSGTAALETAQRAVNIGPGDEVITQTNTFIATALSTFNLFAIPVFIDS
                     DPETFMINADLIEERINENTRAIIPVHIGGAAADMDKIMAIAKKHNLIVIEDACQAHM
                     AEWRNRKLGTIGDMGCFSFQAGKSLCCGEGGAVLGNDEHIMAAAKGYTHIGKDPRNRG
                     RVFAGSNFRMTPFQAAILTGQIRRIEEQSTLRDENAAYLEKLLGEIRGVRPTKKYPGQ
                     TRRGYYIYQLIYDKEYFNGLPKPRFREAMRAEGIPLGNGIDSSLHSDPFIEAYFSHRS
                     FKKIYSKERLDKYRRENLCPVNEMIDRETGLSLGQRVFLGTRKDMEDIVAAIVKIQKN
                     SSALL"
     gene            5620..6477
                     /locus_tag="PLZ75_08705"
     CDS             5620..6477
                     /locus_tag="PLZ75_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOS72511.1"
                     /translation="MKKRTISCTAGISITAAILFCSLTQLAGCSEKEQKYKPVADLPG
                     KYVLVFQDEFDGETLDTTVWGFHAPGKRRSAVNVREACLLNGKGELEIRNWTEISEND
                     TVHHAGMIETRKDFAFGYYEARIKFNIREGSWGAFWIMYRNTGKTYGEKDNPRKDGTE
                     IDIMEFVPLNGRYGCHNLHWNGYGEFIKSRGSGELMNGLLDDYHIYSLLWTRKGYIFY
                     IDGQETWRSGEGVSHVPEYVILSTEIQDGGWAGDIPESGYGSFGETENIMYVDYIRVF
                     RVSSGYRAI"
     gene            complement(6452..>7154)
                     /locus_tag="PLZ75_08710"
     CDS             complement(6452..>7154)
                     /locus_tag="PLZ75_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS72512.1"
                     /translation="DKLKRCPAGNLKLIVVDGVFSMEGDIARLPELVCLCDKYDATIM
                     VDDAHSIGVLGENGSGTASHFGLTGKVDLIMGTFSKSLASLGGFIASDKDTINFIKHN
                     SRSLIFSASITPASAAAVLAAVDIMVSEPERIAHLWEITHYALKGFRETGFDTGKSET
                     PIIPLFIRDDVKTLLLTQALLADGVFVNPVVSPAVPKEDSLIRYSLMATHTKEQIDIS
                     IEKITRAAKSLGILS"
BASE COUNT         1894 a         1670 c         1634 g         1956 t
ORIGIN      
        1 tgcgtcaaca cagcggcgga aatcatccac gctgaagggg tcgtctatat aggcaggata
       61 aacctgtatg ctcggcacaa tcatgccggg agccttcccg tcagcttctt ttaccactga
      121 actgatccag ccggcatcgc gcctgagcat ctgggaataa cacataggag agatataatc
      181 cgcacaggct gccagttttt tcagatcctg accggccacc cttatgttgg ctccgtcaaa
      241 atcagcatcc ctccagggaa ccgcatggat cattatcttc agatcagggt ttaccctgtg
      301 cagggcatca gctattccct ctgccatcga tgcaatcaga ccggtacgat agtcattcca
      361 ctcatcctga taatgatcaa tgatatattc cgatttttgc cccactctta tgcaggaatc
      421 cggaagggaa atcccttgtc cggcaaggaa agcgcctgta cagctgtcac agaagcatgc
      481 catatcaatg cttccggctg ttctgtccgg atagatcatt tcccagaaaa caaaatgcct
      541 tatgaaatca atactcattc cgtccggctt aagttcccgg gcaagatcag ccgcttccct
      601 gattttccgg ttacggaacc catgccttga cggacagaca aactctaccc agctctcccg
      661 ggcttttctt cccctgtccg ttacggcata aagggaactg tcctctttca gggctgcagg
      721 atcataaaaa acaggaaaaa taataaaggt ctttatctca tgctctttca gtacctgccg
      781 gaattcagca ttggcagcca gatccttgct tacaaaggct gtattgatgc ccattttgtc
      841 ccaccgttcc gcgagcaacc cgaaatcccc gtcataatca tagatcttta tgccgatcat
      901 cctttccgga gtcctgtggc tgccgcagcc ggaacccggc atcaggagtg ccagagccag
      961 gaaaacaatt aatacatgtc tcatatccgt ttgtttactc atcatatgcc agacatgccg
     1021 ggtccgtaca gataacggac ctttgcaaac tttcggccgg aggccggagc tttcctgcgg
     1081 ttttataaat ccgcaacaac gaatcttgcc agcggtgcca tccttgcctt gttcagcgac
     1141 agatatttcc cgtcagttga aagattgtcc gcctctgaaa gtacctgcag aaattcctgc
     1201 tcaaggtcat ccggacaagc cataagggtt gacataccat gcccgaatct taccctgaat
     1261 tcattctgta tcttataagg gatccgcagc atgttgcagt tcattttggc agaaacaaag
     1321 ccgtcttcag aatagaaaat catgtaatgc gtttccgggc tgccctccac cggctttcca
     1381 tatatctcca ctatctgcca cctcttgtct tccacactgg tgttgccgtt tttccttaat
     1441 atgtaatagt tctccagctc tccctttatt tcctttccct gcatatccag atatcttacc
     1501 tggccctcct ccaccttgaa ccatggagat ctttcatttt taggaatccc ctctagcctg
     1561 atgttattgc ccttccatgt aaaccttccg cttaaagtgt ctgtatattc attgtctccc
     1621 tttatatggc tgcttgtcag gatataagta aggtcatcat ttattgtcag ttctgtttca
     1681 attctttcac agtcggcaca gggcaggttt cccgagtatg ttccgtgcca gtcgagtgag
     1741 atctgcgcag tatgtccgtc gggggccttt ttaactccgc tgttgccaca gccccagaaa
     1801 cagaatgtga gcatgacagt tgccatgata aggcctgttt ttaaatttat tattttcata
     1861 atgcttaata gtttgttcag accgcaaaat taatgattcc ggcctgttaa ttcaaactgc
     1921 ttcccgggtt tgctgtgtaa agcaatcctg ttccgtaata tctgtccgta tttctttccc
     1981 tattcaaaaa ttgaatgaat cattctgccg gtccagcttt caggggtgct gcacttaaac
     2041 aggtgtgcaa ccacaaaggc agaattcaca gtattatttg gcccggtgat cttgttgttc
     2101 tttttcactc ccggtccggt caggctccat ggaacttcca tctcggtcgg ggtgactcct
     2161 ccgtgaccct tgttgacacc gccgtgatca gaggttacaa tgatgtgggt atcttcaagc
     2221 attccctctt tttccatttt atccagaaga gcgcctatgt gaaaatctgc attctcaatt
     2281 gattcaatgt attcgggaga catccatccg tgactgtggc cggcaccgtc agtatgcacg
     2341 gtgtaaagaa atacgaatgt cggtttttcc ctgttctccc tgataaattc aaatgccctt
     2401 tcaaaatttt caatgtattc atagttttcc tggaaacgga cttcatccag gtaatcacga
     2461 ttataggggt atatcagatt tgcccagtta tagtagaacg ctgttctcac tccgggaacc
     2521 tgatctttca gtattttgaa aattgaagga tagtatccct tgtcatcggt atcaactgcc
     2581 ggtaatacat gattgtcgat aagccagcca ttatcggtaa ccccgtgctg ttcaggaccg
     2641 cttccggtca gatggcttgt ccagttcgga agggttacgg aaggcatgac aactcttgtt
     2701 ttgagtgaaa atgaaccttt ctgcatcagt ttgtcaatat tcggggtttt tgccgtctga
     2761 tagccttcta tgctgatgcc atccagtgct atgaagagca cgcgttccgg tttgccggat
     2821 gaacatgagg ccgcgtaaaa tgcaaaagat atcagaataa aataagccag gaatttgtga
     2881 acgtttgttt ttcttgtttt cattatttgt tgtttagtgt tattgtgccg ggttaaagag
     2941 ccatacttca tcatgcatgg tataaagcct gtatttctct ccgtcataaa ccattatggg
     3001 actggaatga tcaccttcca ctatcgggtt gccgggatat ttgacccagt tgtcaaggtc
     3061 ctcagacatt gccacactgg aggtccagat tgatcctgca ccgggttgtg cccatccgct
     3121 gtccgacgtt ccgtgataat acatataata ttttcctctg aattttatta tctggttcga
     3181 tgcgacagca ccggaatcat attctcctgg acctttcttc agaacaggct catcacttac
     3241 atttgtccag gtaatgtaat catccgaaac agcatgccat attccttcat catttatttc
     3301 ataaaaaaga tgccatttgc cgtcttcaac aagaactgaa ggggttccgt agggaccggg
     3361 aagggtgtcg ccccgggtgg taagtattat cagatcaccc tgttcattcc agttaatgcc
     3421 atcgtcagaa ataagcagat gagcaacatc accggttcct tcggcataca tgtaatattt
     3481 gctgccgtgc cggaaaacaa acatatcttc agtccattta ctgtcaaaca caggattccc
     3541 ctcatacctg gaccagtgaa tgccatcggg agaagtggca taacccagga attttactga
     3601 atttccgccc ccgtcatatc cggtgtacca catcctgtac aatccggctt cgaaaagtat
     3661 gaatcctctt tcccttatgt gcctgtccca ggtgttttcc tttgtcccgg tgaaaacggg
     3721 gttatgctca taaggagtga atttcaccat ttcggccgga aagtcttcac ttacctcatg
     3781 ttttccccgg gatttttgtt tatgcctgca ggatgtcagc actgaaaaca taagcggcag
     3841 aatgatcagg attttaaaaa tgattctcat cgggataatt tttcggttta attaacaaag
     3901 ttaggcttat tcggggaaat tatgcccgga gtccctcctg aatttaccag tgtacccgga
     3961 tctttccgga ctctgaacac tgccttttta ataaaatgcg gccttttgaa tatatttgta
     4021 tctgggcaga ctcacataag cacaggtatt tgataataaa tcctaactta gacaataatt
     4081 ataaatcctg aaaaattata aacctgtaaa attatcatca tgaaaaaacc atctttcagc
     4141 cgcagggaat ttgtgaggaa tacctccctg ggcgttgcag gtgctgctct ttctgcaaag
     4201 ggcataacat cattctctcc ggttgctctt caggataccg gtaagccggc actgctcgga
     4261 ggtacgcctg ttcgtcccaa agacaggata ctcggatcat cctggccgat ttatgatgat
     4321 tccgatatcc agttgtacct cgaggccttt aaaagcaata tatggtcaga atacagccat
     4381 attgaaagtg agagggtcta tcagaccgag aagaaatatg ccgccctgat gggtgtaaac
     4441 tactgtgttg ccaccagctc cggtaccgca gccctggaaa ctgcccagcg cgctgtcaac
     4501 attggtccgg gagatgaggt aatcacccag acaaacacct ttattgcaac tgccctttca
     4561 acatttaacc tgtttgccat tccggtattc attgattccg atccggaaac gtttatgata
     4621 aatgccgatc ttattgaaga gcgcatcaat gaaaatacaa gggcaataat acctgttcat
     4681 atcggaggtg ctgcggcaga catggacaag ataatggcta ttgcgaaaaa gcataatctc
     4741 attgtaatcg aggatgcctg ccaggctcat atggctgagt ggagaaacag gaagctggga
     4801 actatagggg atatgggatg tttcagtttc caggcgggaa aaagtctgtg ctgcggcgag
     4861 ggaggcgctg tgctggggaa tgatgaacat attatggcag ctgccaaggg atatacccat
     4921 ataggaaagg atccgagaaa ccggggaagg gtttttgcag ggtcaaattt caggatgaca
     4981 cccttccagg ctgccatcct taccggccag atcaggagga ttgaagaaca gagcaccctc
     5041 cgtgatgaaa atgccgcata tcttgaaaag ctgctgggtg agatcagggg agtcagaccg
     5101 acaaaaaaat atccgggaca gaccaggaga ggttattaca tataccagct gatctatgac
     5161 aaggaatact ttaacggtct gccaaaaccc agattcaggg aagccatgag ggcagaaggg
     5221 attcctctcg gaaacggaat tgattcatca ctccacagcg atcccttcat cgaggcatat
     5281 ttcagccaca ggtcatttaa aaagatctac tcaaaggaaa gactggataa atacagaagg
     5341 gaaaacctgt gtccggtcaa tgagatgatt gacagggaaa caggcttatc gctgggacag
     5401 agggtgttcc tggggaccag aaaggacatg gaagatattg ttgcagccat tgttaagatc
     5461 cagaagaatt catcagccct gctctgaaag tattgatccg gccctgaaag accggaatac
     5521 catccggcaa atgagtctta atatcgggat aaaggcggat ttattggcgt tacagatatg
     5581 aacggcggcc attaaatatt gaatttaaat tatacgatga tgaaaaaaag aactataagc
     5641 tgtacagccg gaatatcaat aactgctgcg attttgtttt gctctcttac gcagctggcc
     5701 ggctgcagtg aaaaggagca gaaatacaaa ccggtggcgg atcttccggg gaaatatgtt
     5761 ctggtctttc aggatgaatt tgacggagaa acgcttgata caacggtttg gggctttcat
     5821 gcccccggaa agcggcgcag tgcggtgaat gtaagggagg catgtctgct gaacggcaag
     5881 ggggagcttg agatcaggaa ctggactgag atttcagaaa acgacacggt ccatcatgcc
     5941 ggcatgatag aaaccaggaa ggactttgct ttcggctatt atgaagcccg tataaaattc
     6001 aatatcaggg aaggcagctg gggtgcattc tggataatgt accgcaatac cggaaaaaca
     6061 tatggtgaga aggataatcc ccgcaaggac gggaccgaaa ttgatataat ggaatttgtt
     6121 cctctgaacg gccgttacgg atgccataat ctccactgga acggctacgg ggaatttatc
     6181 aagagcaggg gaagcgggga acttatgaac ggccttcttg atgattatca tatttacagc
     6241 ctcctgtgga ccaggaaggg atatattttc tatatagacg gacaggaaac ctggagatcg
     6301 ggggaaggag tgtcacatgt cccggaatat gtcatattaa gcacggaaat tcaggacggc
     6361 ggatgggctg gcgacatacc tgaaagcggc tacggatcct tcggggaaac agaaaatatt
     6421 atgtatgttg attatataag ggtttttcgg gtcagctcag gataccgagc gatttagcag
     6481 ccctggttat cttttctatt gatatatcaa tctgctcttt tgtatgagta gccatgaggg
     6541 aatacctaat cagggaatct tctttgggaa cggcaggtga caccacaggg tttacaaaaa
     6601 cgccgtcggc aagcagtgcc tgggtcagga gcaatgtttt aacatcatca cggatgaaaa
     6661 gaggaatgat cggtgtttct gattttcctg tatcgaagcc cgtttccctg aaccctttca
     6721 gtgcataatg tgtgatttcc caaaggtgtg cgatcctttc cggttcgctg accatgatat
     6781 ccactgctgc cagaactgca gcagctgatg caggagtaat acttgcactg aatatgaggg
     6841 atctggaatt atgtttgata aaattgattg tgtccttgtc tgaagcaata aacccgccga
     6901 gagatgccag tgatttggaa aatgttccca ttataagatc aaccttgccg gtaagtccga
     6961 aatgtgacgc ggtccccgaa ccattctccc cgagtacacc aatggagtgt gcatcgtcaa
     7021 ccattattgt ggcatcatac ttatcgcaca ggcagacaag ttcaggtaac ctggcaatat
     7081 caccttccat cgagaatacc ccgtcgacaa caatcagttt caggttccct gcaggacatc
     7141 ttttcaactt gtct
//
LOCUS       DAOSPX010000163         7141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9787_length_7141_cov_3.754304, whole genome shotgun sequence.
ACCESSION   DAOSPX010000163 DAOSPX010000000
VERSION     DAOSPX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9787_length_7141_cov_3.754304"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLZ75_08715"
     CDS             complement(<1..478)
                     /locus_tag="PLZ75_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOS72513.1"
                     /translation="MKYLKTIPLVMTAVLIFSGCSAKKSDKAPGNVSGERAVIPVRVL
                     ALERTKIARTIDYTATIMPYEEVNMAPSTPGRIDRIYVETGDRVTKGQELFLMDRTQL
                     YQQRVQLSSVQKDLSRLDTLLRTGSATQQQYDQMKTQYDVLKTNVDFMEENTLLRAP"
     gene            complement(506..1840)
                     /locus_tag="PLZ75_08720"
     CDS             complement(506..1840)
                     /locus_tag="PLZ75_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS72514.1"
                     /translation="MKRILFTLTAFLLIGGSLFSALENTDELKLSVREAQDYALMNNK
                     MVLAARSDVQASRAAVWETISAALPQVSANAAIMDNLKLMTTLLPGDFVGRPGEKIPV
                     TFGSQFNSSGAIEASLLLFNAPLYIGIETSRLANRLSQENLLKSELDTKESVSSAYFL
                     ILISEKSLELLNSNIDNLRETLKSTRAMYEVGMAEATDVDQMISNVTMVENNRSTLER
                     TIELNYNLLRFQLGVSPSTSIVLTETLESLTAEMDVEALLSQEFDHTRNVDYRLVEGQ
                     EMISSLALKSQKASVLPTLAGFYNYGTNGMGDKLNDLSWFPNSMVGLQVSLPIFASGQ
                     RYARISKARISLEKAKTTKDMVAEQLLIQEKQLRYNLVNANLQYKNQLDNVEVSKRIY
                     TSVENKYKQGTASSLELTQANSQYLQAENNYVTALMNLLQTRVALEKLLNNL"
     gene            complement(1842..2486)
                     /locus_tag="PLZ75_08725"
     CDS             complement(1842..2486)
                     /locus_tag="PLZ75_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOS72515.1"
                     /translation="MKHRQRITEEAALMFSMYGIRAVTMDMLASRLRISKRTIYEIFH
                     DKDELLAAVLRRMGEKQKELVSRIFSGSENVIEAIFSFFELMDEHYSRMSPAFRLDMK
                     NYHHDIIRRLRETDELPLYSDNAAMIERGIEEGVFRSDIDVTITNKCFFEMLRILLDK
                     DGEGDSDPDRKHIFRDFYINYLRGISTPEGLDLIDFYNRKQGIENQNNSIKKNN"
     gene            complement(2607..3788)
                     /locus_tag="PLZ75_08730"
     CDS             complement(2607..3788)
                     /locus_tag="PLZ75_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOS72516.1"
                     /translation="MAQLVSPDAVSTRYTSYPSAPGTRDQIFIYCNSSGSQKGSLSAR
                     IPENKAPSDFAWYRWNTSASSFSIAVKTETAVRSSSLANLDPGGYRVIISGGHHTIMT
                     AWIVFDETPFVSAKLMNPMKHCNYVALDGTAGAAVSSFGYFDPLDGRALSLKNEVSFL
                     WSSEPESSIPYPSLELDPKIFTPPLVDVVYTLRVNTLGCSSSDTIDYKSIHVKADFTL
                     DPLKGEAPLEVAITDKSVRAHVYEWDFGDDTISTLNDPLIHTYYKPGEYTIRLRVESE
                     RHCIDSVRSAKIIVEPSALAIPNVFSPDGDGYNDRFMVDSRSLRYLTMDVFSRSGRKV
                     YSFSGEGERLKEWTGWDGNINNSSVKAAPGLYFYIIRAYGWDDIRYDSKEYRGFVYLY
                     R"
     gene            4166..6472
                     /locus_tag="PLZ75_08735"
     CDS             4166..6472
                     /locus_tag="PLZ75_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOS72517.1"
                     /translation="MTRDYLVELNESQREAVINTEGPALVIAGAGAGKTRVLTYRIAH
                     LLQKGVHASQILALTFTNKAAGEMKDRIARIAGYETARYLWMGTFHSIFARILRKEGD
                     RLGYQPNYTIYDTSDSKSLIRTIIRELSLDDNIYKPGVIAARISGAKNNLITSGMYAA
                     DNSLHEYDKSCRMPFMADIYKTYSARCFRANAMDFDDLLLNTNILLKDFPEVLSSYQE
                     KFRYILVDEYQDTNFAQYLIIKQLAARHENICVVGDDAQSIYSFRGARIENILKFRDD
                     YPSSRLFKLEQNYRSTRIIVNAANDIIANNEGQIHKTVFSENEEGDRIMIMQAVTDME
                     EGFNIASDIFDKRLTHRLKWEDFAILYRTNAQSRIFEETLRRKNIPYKIYGGLSFYER
                     KEIKDILAYFRMVINPDDEEAFRRSVNYPKRGIGDVTIQKILDLATDMNVTAWSIIND
                     AEILPSYFNAGTIRKISSYTSLINSFVAGREKADAYSLARDIASGSGIMKELRDGKMP
                     DEISRYENLEELLNGIKAFTEAAETNGEPAGLDAYLANVALLTDQDNEKEEDSEKLTL
                     MTMHSAKGLEFNYVYIVGMEDTLFPSPMSSGSLKELEEERRLFYVAVTRAKKQVILSY
                     ALNRYRWGNLERSNPSRFLQEIDKKYIHYPQTGGKPFRSGRPATDKVSFLSEKPAGSI
                     PSAGSIPSARLRRIRTADRTTALHQPSSGPAGTQQFNEGDRVLHERFGQGIIVSLEGE
                     APDNTASVEFENSGRKKLLLRFARLRKI"
     gene            complement(6493..>7141)
                     /locus_tag="PLZ75_08740"
     CDS             complement(6493..>7141)
                     /locus_tag="PLZ75_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72518.1"
                     /translation="LVVFVLMVLKGPMKGLNYSIDSGDIKNMAWNWDGSLEQFFGMAS
                     LGAFALFLGLLALAYALLALPVVKYGAKMIFLESVRKVKPDFEWLIRGFWTGYLKIIL
                     ANLLVFALIMIALFALIVPGIIVACRLAFVPYIVMDKKLDPIESVELSWKLTRGRGWT
                     IFLMGFVAFFIYIFGFALFIIGIFPAVMWVKSSFASLYQSVLLAEEKPAVEELLA"
BASE COUNT         1953 a         1719 c         1604 g         1865 t
ORIGIN      
        1 agggagccct gagcagggtg ttttcctcca taaaatcaac atttgtcttc agcacgtcat
       61 attgtgtctt catctgatca tactgctgct gtgtggcgct gccggtccgc aaaagcgtat
      121 caagcctcga aaggtccttc tgaacactgc tcagctgaac cctctgctga tacagctgcg
      181 tgcggtccat aagaaagagt tcctgcccct ttgtcaccct gtcacccgtt tcaacatata
      241 tcctgtcaat cctcccgggt gtggagggtg ccatgttgac ttcctcgtaa ggcataatag
      301 ttgccgtata atcaattgtt cttgctattt tcgtcctttc aagagccagt accctcaccg
      361 gaatgactgc cctctcgccg gatacattgc ccggggcctt atctgatttc tttgcagaac
      421 agcctgaaaa aatcagaact gccgtcataa caagcggaat tgtctttaag tatttcatcg
      481 tatttgtttt tgtatttcca ggtaattaaa gattgttaag aagtttttcc agggcaaccc
      541 tggtctgaag aaggttcata agcgctgtca cataattgtt ttcagcctga agatactggc
      601 tgttggcctg tgtgagctcc agactggatg cagttccctg tttgtattta ttctccacgc
      661 ttgtataaat ccttttcgac acctcaacat tgtcgagctg gtttttgtac tgaagattgg
      721 catttacaag attgtacctc agctgttttt cctgtatcag aagctgttca gccaccatat
      781 cctttgtggt ttttgccttc tcaagactga ttcttgcctt gctgatgcgc gcatacctct
      841 ggccactggc aaaaattggg agtgatacct gcagccctac catggaattc ggaaaccatg
      901 acagatcgtt tagcttgtca cccataccgt ttgtgccgta attatagaag ccggcaagtg
      961 tgggaagtac cgaagctttc tgcgatttga gcgcaaggga ggatatcatt tcctggccct
     1021 ccacaagcct gtaatctaca ttcctggtgt gatcaaattc ctgtgacaga agagcttcaa
     1081 catccatctc tgctgtaagg ctttccagtg tttcggtcag tactatggag gttgacgggg
     1141 aaacgcccag ctgaaacctg agaaggttat aattcagttc gatggtgcgt tccagtgtgc
     1201 tgcggttgtt ttcaaccatt gttacgtttg aaatcatctg gtcaacatcc gttgcctccg
     1261 ccattcccac ctcatacatg gcccgggtcg atttaagcgt ttctctcagg ttatcaatat
     1321 tgctgttgag cagttcaagg gatttttccg aaatgagaat caggaaataa gcagagctta
     1381 ccgattcttt tgtgtccagc tctgatttca ggaggttctc ctggctcagc ctgttggcca
     1441 gcctgctggt ttctatgcca atgtataaag gtgcattgaa aagcaggaga ctggcttcta
     1501 tggctccgct ggaattgaac tgcgatccga aagtgacagg gatcttttca ccgggccgtc
     1561 cgacaaaatc acccggcaga agggttgtca taagtttcag attgtccatt attgccgcat
     1621 ttgcactcac ctgaggaaga gctgccgata tcgtttccca tacggccgct cttgaagcct
     1681 gaacatccga tctggcagca agaaccatct tgttgttcat cagggcataa tcctgtgcct
     1741 ccctgaccga aagcttcagc tcgtcagtat tttccagggc actgaagagt gaccccccga
     1801 ttagcagaaa tgccgtaagc gtaaatagaa tccgtttcat attaattatt tttctttatg
     1861 ctgttgttct gattttctat tccctgcttc ctattgtaaa aatcaatcag atcaagacct
     1921 tcaggcgtgg aaattcctct caggtaatta atgtagaaat ccctgaaaat atgcttcctg
     1981 tccggatcac tatccccttc gccgtccttg tcgagcagta tccgcaacat ttcaaaaaag
     2041 cacttgttgg tgattgtaac gtcaatgtca ctgcgaaaga caccctcttc aatccccctc
     2101 tctatcatcg cggcattgtc gctgtacagg ggaagctcat ccgtttccct gagcctgcgg
     2161 attatgtcat gatgataatt cttcatgtca agtctgaaag cgggactcat acggctgtaa
     2221 tgttcatcca tcagctcgaa aaaactgaat atggcttcga tgacattctc cgaaccggaa
     2281 aaaatccttg aaacaagttc cttctgcttt tctcccatcc gccggagaac agccgcaaga
     2341 agctcatcct tgtcgtgaaa aatctcgtaa atggttcttt ttgatatcct cagacggctg
     2401 gcaagcatgt ccatcgttac ggcccttatg ccatacatgc tgaacatgag ggctgcttct
     2461 tccgttatcc tctgcctgtg tttcattgcc tgtccgtaaa aacgcggcaa aaatgaaaaa
     2521 acttttttag tttacaaagt tttcatgcgt aaatattgga aatttacctg tgaattgagg
     2581 aattatgagg gcgaatgccc ggctgtttat ctgtacagat atacaaatcc ccggtattcc
     2641 ttgctgtcat atcttatatc atcccatccg tacgcccgta ttatatagaa atacaatccc
     2701 ggggcagctt tgaccgatga attatttata ttgccgtccc agccggtcca ttccttcagc
     2761 ctttcaccct ccccggagaa gctgtatacc ttccttccgg agcggctgaa aacatccatg
     2821 gtcagatatc tcagcgacct ggaatcaacc atgaacctgt cattgtatcc gtccccgtcg
     2881 ggactgaaaa catttggaat ggcaagagct gacggttcca cgatgatttt tgccgaacgt
     2941 accgaatcaa tgcagtggcg ttcgctttca accctgagac ggatggtata ttccccgggc
     3001 ttatagtagg tgtgtatcag cgggtcgtta agcgttgaaa tggtatcatc gccaaagtcc
     3061 cattcgtaaa catgtgccct gacggactta tccgttattg ccacttccag tggtgcttct
     3121 cccttcagcg gatcgagggt gaaatccgcc tttacatgaa tcgacttata gtcaatcgtg
     3181 tcggaactgc tgcaccccag cgtgtttacc cgcagagtgt aaacaacatc aaccagcggc
     3241 ggtgtgaata ttttcggatc aagttcgaga gagggatagg ggattgatga ttccggctcc
     3301 gacgaccaga gaaagctcac ttcatttttc aggctcaggg cacggccgtc aagggggtcg
     3361 aaatatccga aggagctcac agcggcaccg gcagtaccgt cgagggcaac ataattgcaa
     3421 tgcttcatag gattcatgag ctttgccgaa acaaacggag tttcgtcaaa tactatccat
     3481 gccgtcatga ttgtgtgatg gccgcctgat atgatcaccc tgtagcctcc cggatcaaga
     3541 ttggcaagcg atgaactccg tacagccgtt tcggttttta cagctatgct gaaactgctg
     3601 gctgaagtgt tccagcggta ccaggcaaaa tcagaggggg ctttattttc cggaatccgg
     3661 gcactgagac ttcctttctg cgaacctgaa gaattgcagt agatgaaaat ctgatccctg
     3721 gttccgggtg cagaaggata ggaagtatat ctcgtggaaa ctgcatccgg cgaaaccagc
     3781 tgggccaaca ggcaataatg tacaaacagc aggaatattg aaataccgaa acgcacaggc
     3841 agtaaaggat ttttctaata aacggttaca ttgaccctat ttgtataaca ctctgcaata
     3901 ttaataaaaa tatgttttaa atccacagtt tcccggtatg atgctattcc ccgtcatgat
     3961 gcgattcccc ggatacagtg acttccctga ataaggcggt atccgcgggg gactgggtcc
     4021 ggccgcatga ctgacaaaga attcacataa gggaagcatt attcttttgt tgaaaaatat
     4081 atgatataaa cagaccgctt tctcttccgc tgaaattgat ttcattaaat ttgccatccc
     4141 ggcaatctgt aataccagct aactcatgac ccgtgattat cttgtcgaac ttaatgaatc
     4201 ccagcgggaa gcggtcatca acaccgaggg gccggcactg gtcattgccg gagcaggcgc
     4261 cggaaaaacc cgtgtcctta cttacaggat agcacacctt ctgcagaagg gtgtgcacgc
     4321 cagccagata ctggccctta cctttaccaa caaggctgcc ggtgagatga aggacaggat
     4381 tgcccggatc gccggttacg aaactgcaag gtatctgtgg atgggaacct ttcattcaat
     4441 cttcgcaagg atactcagga aggaaggcga caggctggga taccagccga actacaccat
     4501 ctatgataca tctgactcta aaagccttat ccggacaatc atcagggaac tcagcctgga
     4561 tgacaacatt tacaaacccg gggtgattgc agcccggatc tcaggtgcca agaataatct
     4621 cataacatcc ggaatgtatg ccgccgacaa ttccctgcat gaatatgaca aatcgtgcag
     4681 aatgccgttt atggccgata tatataaaac ctattcggcc aggtgcttca gggcaaatgc
     4741 aatggacttc gatgacctcc ttctcaatac aaacatactg cttaaggatt ttccggaagt
     4801 tctttcatca taccaggaaa agttcaggta cattctggtt gatgaatacc aggatacaaa
     4861 tttcgcccag tatctgataa taaaacaact ggcagcccgg cacgaaaata tatgtgttgt
     4921 aggggatgat gcacagagta tttactcctt caggggagcc cggattgaaa acattctgaa
     4981 attcagggat gattacccct cttcccgcct tttcaagctg gaacagaact acagatccac
     5041 ccgaatcatt gtaaatgctg caaacgacat aattgccaat aacgagggcc agattcacaa
     5101 aactgttttc tcggaaaatg aagagggcga caggataatg atcatgcagg cggtgacaga
     5161 tatggaagag ggattcaata ttgcatcgga tatctttgac aaaaggctta ctcaccggct
     5221 gaaatgggag gattttgcca tcctttacag gacaaatgcc cagagccgta tttttgaaga
     5281 aacactgaga agaaagaata taccttacaa aatatacggg ggcctttcat tctatgaacg
     5341 taaggagata aaggacattc tggcctattt caggatggtc atcaacccgg atgatgagga
     5401 agccttcaga agatcggtga attatccgaa aagaggcatt ggggacgtta cgattcaaaa
     5461 aatcctggat ctggcaacag atatgaatgt tactgcctgg agcatcatca atgatgctga
     5521 aatattacca tcatatttca atgcaggaac tatcagaaag atttcatcct atacctccct
     5581 catcaactca tttgtagccg gcagggaaaa agcggatgcc tattccctgg ccagggacat
     5641 tgcatctggc tcgggaatca tgaaagagct gagggacgga aaaatgccgg atgagataag
     5701 ccgctacgaa aacctggaag aacttctgaa tggtatcaag gcatttaccg aagccgctga
     5761 aaccaatggt gaacctgcgg gacttgacgc ataccttgcc aacgttgccc tgctcaccga
     5821 tcaggataat gaaaaggaag aagatagtga aaagctcacc ctgatgacga tgcattctgc
     5881 caaaggactg gagttcaatt atgtatatat agtaggaatg gaagataccc ttttcccgtc
     5941 cccgatgtct tcaggaagtc taaaggaact ggaggaagaa aggcggctct tctatgtggc
     6001 tgttacaagg gcaaagaaac aagtcatcct gagctatgca ctcaacaggt acaggtgggg
     6061 aaatcttgaa agaagcaatc ccagcaggtt cctgcaggaa atcgacaaaa aatatatcca
     6121 ttatccacag accgggggaa aaccattccg cagcggaagg ccggcaactg acaaggtttc
     6181 cttcttaagt gaaaaaccgg ccggcagtat tccttcggcc ggcagtattc cctccgcccg
     6241 cctgagaagg atccgcacag ctgacaggac aactgccctg catcagccgt catccggacc
     6301 ggcagggact cagcagttca atgagggtga cagggtgctt catgaaagat tcggacaggg
     6361 tatcattgtt tctcttgaag gagaagcacc tgacaatacg gcatcagttg aatttgaaaa
     6421 cagcggcaga aaaaaactgc tgctgagatt tgcccggctc cggaaaatct gaataattca
     6481 agtctttgtt ttttatgcca aaagttcttc aacagcaggt ttttcctctg caagcagtac
     6541 agactgatac aatgatgcaa atgaactttt cacccacatt acagccggaa aaataccgat
     6601 aataaaaagg gcaaaaccaa aaatataaat gaaaaacgct acaaatccca tcaggaatat
     6661 tgtccagccc cggcctcgtg tcagcttcca gctcagctca accgattcaa tcggatcaag
     6721 ctttttatcc attactatat aaggtacaaa agccagccgg catgccacta ttattccggg
     6781 tacaatcagt gcgaacaaag ctatcatgat cagtgcaaaa accagcagat tggccagaat
     6841 gattttaagg taacctgtcc agaatcctct gataagccat tcaaaatccg gtttgacctt
     6901 ccttactgat tccagaaaga tcattttcgc tccatatttt accacaggca gggccagaag
     6961 tgcataggcc agagccagta atcccaggaa taatgcaaat gcccctaggg atgccattcc
     7021 gaaaaactgc tccaggctgc cgtcccagtt ccaggccatg ttttttatgt ccccgctgtc
     7081 aatactatag ttcaaaccct tcatcgggcc tttcagaacc atcaggacaa aaacaaccag
     7141 c
//
LOCUS       DAOSPX010000164         7001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_9993_length_7001_cov_4.081054, whole genome shotgun sequence.
ACCESSION   DAOSPX010000164 DAOSPX010000000
VERSION     DAOSPX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9993_length_7001_cov_4.081054"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..648
                     /locus_tag="PLZ75_08745"
     CDS             160..648
                     /locus_tag="PLZ75_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS72519.1"
                     /translation="MKQKLLKLSAMLLFCLGLTGLQAQESVNTTGGNASGSGGTVTYS
                     VGQLVYQTHATQFGSMAHGVQQPYEISVVTGIEEAVGIKLSLSAYPNPTTDYLTLEVK
                     DFDLSALYFYLYDMNGKLLQNEKIKSIQTNIDMSNLEPGSYFIKVIRKNKEFKTFKVI
                     KN"
     gene            707..1612
                     /locus_tag="PLZ75_08750"
     CDS             707..1612
                     /locus_tag="PLZ75_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72520.1"
                     /translation="MKTKMQKFKAVLIITLTNLLICISVFAQLPQKISYQALIRDNNN
                     DLVTNRLMGMQINIRKGNIKGPVVYTETQTPTTDANGLVSIQIGGNDDFNMIDWPAGS
                     YFIETRIAIIPPYTTYTITGTSQLLSVPYSLHSKTAEHAKTTEKFTSVRKLGEEYLGG
                     IIFYLYIGSDGEQHGLVVSKTETNAYWQSSLSLVNADRTDDGYYNTERMINSPAKTWI
                     TGLGTGWYLPSIDELYLLWDNRFHVDKTLRAIGSAGFARRGYYWSSTETSLSTACSIS
                     FSAGIVESLSSKGLNYYFVRAVRAF"
     gene            1818..2720
                     /locus_tag="PLZ75_08755"
     CDS             1818..2720
                     /locus_tag="PLZ75_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72521.1"
                     /translation="MKTKIQKFKAVLIITLTNLLICISVFAQFPQKINYQTVIRDKDN
                     ELVTDKLIGIQINIRKGSVRGPVVYTENRTLTTNANGLVSIQIGGNDDFNMIDWPTGT
                     YFIETGIAIFPPYNTYTISGTSQLLSVPYSFHSKTAENSKTAEAFTSGRYLGEEYLGG
                     IIFHLYIGSDGQQHGLVVSKTEARAKWQNTAGHLAGADRTDDGYYNTARMTDSPAKTW
                     ITGLGTGWYLPSVDELALLLRNRFHVNKTLRTIGSAPLLSTDYHWSSTEWSESAAFYI
                     RFLNYIVNTENKEKEYVIRAVRAF"
     gene            3058..3930
                     /locus_tag="PLZ75_08760"
     CDS             3058..3930
                     /locus_tag="PLZ75_08760"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOS72522.1"
                     /translation="MTRNATHHSGEIYVIKANGEKEPFRFSKLESSLRNAGTDNAAIR
                     EIINDIEGWIYPGVTTKKIYSRAFGLLKRIRGVAAVRYRLKQAIYDMGPTGYPFEYFI
                     GRIFELQGYSVQTDQIKQGKCVTHEIDVVATNDHEHHLVECKYSQARGRRIPVQTPLY
                     VKSRFDDILAQYRIDNLFSGYRFFGWVVTNNRFSSDSISYGECAGLKLMSWDYPAGHS
                     LKDIIERENIYPITVLTKITNREKQLLLEKGIVTCAGLLDNLDVLDSLHFTSSRSTAL
                     LRELHDIATFRPEY"
     gene            complement(4112..4546)
                     /locus_tag="PLZ75_08765"
     CDS             complement(4112..4546)
                     /locus_tag="PLZ75_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOS72523.1"
                     /translation="MADLMFKISAHSASPAKTIVKARNFQIIVDEPASLGGSNEGPNP
                     VEYVLASLAGCLNVMGHVVAGEMGFELRGVKIDISGDLNPDRLFGKSFDERAGYKNIE
                     VKMTPDCDTDKDTLEKWIETVDERCPVSDNLSNATPIQFKLA"
     gene            4709..5374
                     /locus_tag="PLZ75_08770"
     CDS             4709..5374
                     /locus_tag="PLZ75_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOS72524.1"
                     /translation="MTIRETHKKQWFDFLTDKQQEQLRASASEVVFRKGETIIKQGTA
                     VTHIYLLNSGLVKLASEDGNRMTVFKIVAANTFVGLMCSFARKNYDFSALAINTSVVR
                     IIDRGVFEQIIRENGEFAMHIIELMSGFTVKIVSDLIGLSHKQANGATATILLDLAEI
                     FNSHSFRMPFNRDEFADTVGFSKESVIGSLTAMQKDGIISVSGKNIEILEPGRLLLVS
                     RHG"
     gene            5388..5483
                     /locus_tag="PLZ75_08775"
                     /pseudo
     CDS             5388..5483
                     /locus_tag="PLZ75_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            5860..6264
                     /locus_tag="PLZ75_08780"
     CDS             5860..6264
                     /locus_tag="PLZ75_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOS72525.1"
                     /translation="MTAVTGLQCNREKLLYKSWILEQYGPEDQLVSITFPSQGDLPVK
                     KQIVLNLAEDGKFSGNDGCNSIFGSFKSGHCHKIEFMNISSTLMMCRQEVMSQAGAIR
                     GILRNVKKYKVNETSLLLLTDNREVLKYMAKQ"
BASE COUNT         2161 a         1439 c         1467 g         1934 t
ORIGIN      
        1 ttacttccgc tcagagcaga ctccgtcgga atcgccttca gtgcaggact tcgtttagcg
       61 tggtgttggc gcttattgca ttaaaacgaa acaaaccaga caaagaggaa tatgaccatt
      121 aattaaaata agtattaacc tttaaaaact atgacgagaa tgaaacaaaa gctattaaaa
      181 ttaagtgcca tgctattgtt ctgtctcgga ctgacagggc tgcaggcaca ggaaagcgtt
      241 aacaccacag gcggcaacgc ttcaggcagc ggaggcacgg tgacctattc cgttggacag
      301 cttgtatacc aaactcatgc tactcaattt ggctcgatgg cacatggagt tcagcagcca
      361 tatgaaattt cggtagtaac aggaattgaa gaagcggtag gaattaagct ttcgctctct
      421 gcatatccca atccaaccac cgattacctt acattggaag tgaaagactt tgatctttct
      481 gctttatact tttacctgta tgacatgaac ggaaaacttt tacaaaacga aaaaattaaa
      541 agtattcaga caaacattga tatgagcaat cttgaacccg gttcttattt tattaaagtg
      601 atccggaaaa acaaagaatt taaaacattt aaagtgatta aaaactaatt tatttgacga
      661 ttgactgatg tgttgtaagc agcaataatt tcaatatttt aaaattatga aaacaaagat
      721 gcaaaaattt aaggcagtat taatcattac attaacaaat ctgctcatct gcatatctgt
      781 ttttgctcaa ttaccgcaga aaataagtta tcaggcatta atacgtgaca acaacaatga
      841 tctggtaacc aacagactaa tggggatgca aataaatatc cgaaaaggta atataaaagg
      901 accggtggtt tacaccgaaa cccagacgcc aaccaccgat gccaatggat tggtaagtat
      961 tcaaataggc ggaaacgacg attttaatat gattgactgg ccggcaggat cttatttcat
     1021 tgaaacaaga atagccatta ttcccccata taccacttac accattacag gcacaagcca
     1081 gttattaagt gtgccttatt ctttacattc aaaaaccgca gaacatgcaa aaaccaccga
     1141 aaagtttact tcagtacgca aactgggtga agagtacctc ggcggcatta ttttttacct
     1201 atacattggt agcgacgggg aacaacacgg tttggtagtt tctaaaaccg aaacaaatgc
     1261 atattggcaa agctccttat ctctggtaaa tgcggataga acagatgatg gatattacaa
     1321 tacagaaaga atgattaata gtcccgccaa aacctggatt acagggctgg gcacaggctg
     1381 gtacctgcca tccattgatg agctatactt gctctgggac aaccgctttc atgtggacaa
     1441 aacgctcagg gcaattggtt ctgcagggtt cgcacgcagg ggatattatt ggagtagtac
     1501 ggagaccagt ctgtccactg cgtgcagcat ttctttcagt gccggcattg tagagtcctt
     1561 aagtagtaag ggcttaaatt attattttgt ccgtgccgtt cgggcttttt aactatttac
     1621 cctgtgagaa aggtacttca tctgatataa ataatagcta aactcaaaat gatggaaaaa
     1681 tcaggatttt atccgcaaac aaatcgcccg gcaagcaccg gtttaatcgc agatattatc
     1741 agcatcaaaa aatcattgat tcaacaattt caactatgaa aaattaaaaa acagcaataa
     1801 tttcaaaatt taaaattatg aaaacaaaaa tacaaaaatt taaagcagta ttaatcatca
     1861 cattaacaaa tctgctcatc tgcatatctg tttttgctca atttccgcag aaaataaatt
     1921 atcagacagt aatacgcgat aaagacaatg aactggtaac cgacaaacta atagggatac
     1981 aaataaatat ccggaaaggt tctgtaagag ggccggtggt ttacacagaa aaccgtacac
     2041 taaccaccaa tgccaatgga ttggtaagta ttcaaatagg cggaaacgac gattttaata
     2101 tgatcgactg gccgacagga acttatttca ttgaaacagg aatagccata tttcctccat
     2161 ataacactta caccatttca ggcacaagcc agttattaag tgtaccttat tcttttcatt
     2221 caaaaaccgc tgaaaattca aaaaccgccg aagcctttac ttcaggacgc tacctgggtg
     2281 aagagtatct cggtggcatt atttttcacc tatacattgg cagcgacggg caacaacacg
     2341 gtttggtagt ttctaaaacc gaagcacgtg ctaaatggca aaacaccgct gggcatttag
     2401 caggtgcgga tcgaacagat gatggatatt acaatacagc acgaatgact gatagccccg
     2461 ccaagacctg gattacaggg ttaggcacag gctggtacct gccatccgtt gatgagctgg
     2521 ccttgctctt gcgcaaccgc tttcatgtga acaaaacgct caggacaatt ggctctgcac
     2581 cgctcctatc cactgattat cattggagta gcacggagtg gagtgagtct gctgcatttt
     2641 acattcgttt ccttaattac attgtaaata ctgaaaacaa ggaaaaagaa tatgtaatcc
     2701 gtgccgtccg ggctttttga agaaccagaa atgcgggttt gacagttcag agcggatgga
     2761 ccttccggcg acccttcgtt ttgagagtac cggaaccggt ataaaatcat tcagtatgaa
     2821 ttttatgaca tcggccggtt ctcctttgtt aaggatgaac cctgcccggt acgagaccca
     2881 aaaagatcag gatcgttgca ttgttaaatg gtcaaagagc cgggtgggga atggatagca
     2941 agaccagaga agacatactg gacaaaatca gaaaatacgg ctatctttag ctgctgacat
     3001 attccaattc ccgaattctg attatcttgt gttttgctta atcaggaaca aaatgaaatg
     3061 accaggaatg ctacgcatca cagcggggag atatatgtaa taaaggcaaa cggggagaag
     3121 gagcctttcc ggttttccaa gctcgagtct tcactgcgta atgcgggaac cgacaatgcc
     3181 gccatcaggg agataataaa tgatattgaa gggtggatct atccgggagt gaccaccaaa
     3241 aagatatatt cccgtgcctt tggtttgctg aaacgcatca ggggtgtggc tgccgttcgc
     3301 taccggctga agcaggccat ttacgatatg gggcctacgg gctatccttt tgagtatttt
     3361 atcggcagga ttttcgagct tcagggatat agtgtacaga cggaccagat taaacagggc
     3421 aagtgtgtca cccacgagat tgatgtggtt gccaccaacg accatgagca tcacctggtg
     3481 gagtgcaagt acagtcaggc ccggggcaga cgcataccgg tgcagacgcc cctgtatgtc
     3541 aaatcgcgtt ttgacgatat cctggcacag tacaggatcg acaatctttt cagtggttac
     3601 aggtttttcg gctgggtggt taccaacaac cgtttctcat ccgattcaat aagctacggg
     3661 gaatgtgccg gactgaaact tatgagctgg gactatcctg ccggccacag tcttaaagat
     3721 attatagaaa gggaaaatat ctatcccata acagtattga caaaaatcac caacagggaa
     3781 aagcaattgc tgcttgaaaa gggcattgtg acctgtgcag gtctgcttga taatctcgat
     3841 gtgctcgact ccttacattt taccagcagc agatccactg ccctgctcag agagcttcac
     3901 gatattgcca ccttccggcc ggagtattga actccagaca tccaaggcct catcagcgtc
     3961 ggaatgggct gcaaaccaaa aaaccaattc agccgggcaa ggggtggaaa atatgttcga
     4021 cctgtcaaaa ttgttcaaaa aatgatgcca gacaagctgt ccggcatcat tgtctgttcc
     4081 ggccctcctg ccggagaacc tttccctgtt actaagcaag tttaaactga atcggcgtag
     4141 catttgagag gttatcactc accggacacc tttcatcgac agtttcaatc cacttctcca
     4201 gggtgtcctt gtcagtgtca cagtcgggag tcatcttaac ctcaatattt ttgtatcctg
     4261 ccctttcgtc aaagctcttg ccaaaaaggc ggtccggatt cagatcgccg gagatatcaa
     4321 ttttcacacc gcgtaattca aaacccattt ctcctgcaac gacatgtccc attacattga
     4381 gacatcccgc cagtgatgcg agaacatact caaccggatt gggtccttcg tttgatccgc
     4441 caagactggc aggttcatcg acaataatct ggaaattacg ggccttaaca atcgtttttg
     4501 cgggacttgc actatgtgcg cttattttaa acattaaatc tgccataatt ctaacctttc
     4561 tttctttaat atgttagtga attaaaattc gagtgtaaaa ttactctaaa gacgggatac
     4621 tcccgggaaa aaagagttga caattacacc gggaataaag gaatataata tcaataaatc
     4681 tgtaatatta cattttttga atagccccat gacaatcagg gaaacgcata aaaagcagtg
     4741 gtttgacttt ctcaccgaca agcagcagga acagctcagg gcttctgcca gtgaagttgt
     4801 gttcagaaag ggggaaacca ttatcaagca gggtacggcg gtcactcata tatacctgct
     4861 gaacagcgga ctcgtaaagc tggcttcgga agacggcaac cgcatgacag tgtttaagat
     4921 cgttgccgcc aatacctttg tcgggctgat gtgttccttt gccaggaaga actatgattt
     4981 ttccgcactg gccatcaaca ccagtgtggt aagaatcatt gaccgcgggg tttttgagca
     5041 gatcatccgc gaaaatggtg agtttgccat gcatattatt gagcttatgt cgggctttac
     5101 cgtcaagata gtatccgacc ttatcgggct gagccataag caggccaacg gagctactgc
     5161 cacaatactg ctcgacctgg ctgaaatttt caacagtcat tcttttcgca tgcccttcaa
     5221 ccgcgatgaa tttgccgata ctgtgggttt ctcgaaggaa agtgttatag gcagcctcac
     5281 cgctatgcaa aaagacggta tcatcagtgt ttccggcaaa aatattgaga tactcgaacc
     5341 cgggcggctc ctccttgtat cacggcacgg atagcctgtg ccagttatgc cgtgattacc
     5401 atatgattgt ttctattccg ccaggagtgt ctgtatcaga cttcatgggt atcttgaaag
     5461 gtaagacggc aataaagttg tttaaaagcc accttccctg aagatggtag tttactttag
     5521 aatttgcaga acagattgtc tgtttatcac agaccttata gtcaggaacc tttgtataaa
     5581 atattcttga catgaacagt tttattgtat aaattaccgg tataaatatg aaattgacgc
     5641 atacagaagc tatcagcaat ctgcttgatt ccgggataga aatagtagat aaggttacat
     5701 gtcggataaa acagataaga aggataaaaa caggtgttgt aaatgtgttt taaatcttcg
     5761 aaattggaat tataaattct tccactaacc agatgactaa gaatgaaaac tttaaagatt
     5821 aagaaccgta tcaataaagt catattaatt ctgacagtat tgacagctgt aactggttta
     5881 caatgtaacc gggagaaact gctgtataaa tcctggattc tggaacaata cggccctgaa
     5941 gatcagctgg tcagtatcac tttcccttcc cagggtgatt tgccggtaaa aaagcagatt
     6001 gttttaaatc tggctgaaga cggtaaattt tccggtaatg acgggtgtaa ttcaatattt
     6061 ggcagtttta aatcaggcca ttgccataaa attgaattca tgaatatctc ctccaccctg
     6121 atgatgtgca gacaggaggt catgtcccag gcaggcgcaa taaggggtat tctcaggaat
     6181 gtgaaaaaat ataaggtaaa cgaaaccagt ttattattgc tgactgacaa cagggaggtt
     6241 ttaaaataca tggcgaaaca gtgaatgaaa taactgcagc cggatattaa aatattcatg
     6301 attgagtagt agaatcatat ttattgcatg tgaagcaaaa ctcgtttctc tttgtattcc
     6361 tcagtctgtt gtcaggtggc atattgcttt tttcgtggtt caatccatca ggcttttacc
     6421 tgattttcat agcatttgta ccctttttat ttgtgattga aaatctgaca attgtcagat
     6481 caacagcagt acgttctgca ttactcattt tacccttttt tatatggaat tttaaaaatt
     6541 tatggatttt taaaatcaat gccacagcct gtatactggc cataatcttg aacactctgg
     6601 ttatatctgc accatggatt attttttcca ggtttcaaaa aagaacaagg atagcattaa
     6661 taaaatattc ctttctgata atctcagtat tgtcaatgga aatatttcat aaaacctggc
     6721 aattatccta cccgtttcat ttgttgggaa atgcattttc agtggctcct gatacaatac
     6781 aatggtatga atatacaggg atttacgggg gcacggtgtg gattttaata tccaatatac
     6841 tggtattttc catatttata aatttcaaat cggatcattc taaaacaatt gtcttgcgta
     6901 ttcttctttt catgttgatt ctgatatttc ccctgataat atcaaaacac ataagaaacc
     6961 gctttaaacc cggtgaatca atcccgcttg aggttgttgc a
//
LOCUS       DAOSPX010000165         6962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10053_length_6962_cov_3.183292, whole genome shotgun sequence.
ACCESSION   DAOSPX010000165 DAOSPX010000000
VERSION     DAOSPX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10053_length_6962_cov_3.183292"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLZ75_08785"
     CDS             <1..897
                     /locus_tag="PLZ75_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOS72526.1"
                     /translation="SLESYRETLKNAGEKADIALFIQNMKSMNLRNSVFIDCTANEKV
                     AARYTEILEAYISVVTANKIACSSDYSYYHQLKSTAGERDVRFIYETTVGAGLPIIKT
                     INDLVLSGDKILKIEAVLSGTLNFIFNEMSGSVPLSKAIRKAREKGYSEPDPRIDLSG
                     TDVVRKILILARESGYKLEYSDVVVSRFLPEECFRGDISDFYRKVEALDDEFEKKRID
                     LEKQNRKWRFTATLDHGKAKVELAEVGPGHPSFFLEGSNNIVLLTTERYCDLPMVIKG
                     YGAGADVTAAGVFADLMRVVNV"
     gene            954..2312
                     /gene="thrC"
                     /locus_tag="PLZ75_08790"
     CDS             954..2312
                     /gene="thrC"
                     /locus_tag="PLZ75_08790"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOS72527.1"
                     /translation="MKERIRYYSTNLKAPEVSFREALMKGIAPDGGLYMPRFIPSLSP
                     EELMGYRGQDYPGMAFSFLYKFLKTEIESDVLNSLCRDAYNFDIPLEPAGERSYILRL
                     DQGPTASFKDFAARMMARLMNHFIAGENRHYTILTATSGDTGSAVASAFHGLSNINVI
                     ILFPLGEVSDTQRKQMTTLHGNVRVIGIRGKFDDCQRIVKKAFLDKSLSDLNLTSANS
                     INIGRLLPQSVYYIYAWSRTGLLPVVFSVPSGNYGNLMGGIIAREAGLPVRKFIIATN
                     ENNEVPEYLRTGSYSIIVPSRNCISSAMNVGHPSNLARIVALYGGSMNEHGTILKSPD
                     LERMRRDFHGVSVTDDTTRETIASCHRDHGVLLEPHGAVAWKGLEHYLAKAGPEEKNG
                     TVYISLETAHPAKFPGEIERILSFTPPGHPSLENLEKLEEHYTRMDNDYGSFTQYVFK
                     HC"
     gene            2323..2940
                     /gene="thrH"
                     /locus_tag="PLZ75_08795"
     CDS             2323..2940
                     /gene="thrH"
                     /locus_tag="PLZ75_08795"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF010109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HOS72528.1"
                     /translation="MYIICSDLEGVLVPEVWINVAEKTGIDELKLTTRDINDYDVLMK
                     RRLEILSQNGISIGDIRNVISGLEPLPGALDFISWLHGKAQLIIVSDTFREFADPMLK
                     KMGWPLLLCHHLTIDRHGMITDYNLRQKEAKMKVVEALQRLNFKVIAIGDSYNDIQML
                     RKAETGILFRPPQNVIDDNSDLLAVDTYDGLKEVISEKLETDGLS"
     gene            complement(3007..3855)
                     /locus_tag="PLZ75_08800"
     CDS             complement(3007..3855)
                     /locus_tag="PLZ75_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72529.1"
                     /translation="MKKLLILPLVFILLSSAVRSQNLVFPDLHGFRTVTEYPVYLPDN
                     LWDFINGAADGFLALGFKDLHVAEYRKGKNSIKLEIYRHENHTMAFGIYASERSPHFN
                     FIRLGAQGYNADGAINFFKGSYYVKIRTYSGKEKILQAAETLAFRVADMLPGENSLPA
                     LLTRFPAEGKLANEETYINDNVLGHSVLNSAFMAPYESGNDSFSIYLIQKESAGEIRD
                     MAESYLNTAGMETLPGDENRFVFNDGYNGTVFLSWKDNLMVIISGLAKDQTAIAYRYT
                     SRILEQ"
     gene            complement(3876..5054)
                     /locus_tag="PLZ75_08805"
     CDS             complement(3876..5054)
                     /locus_tag="PLZ75_08805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS72530.1"
                     /translation="MEIISRRGFLKKGITGAGALAVSPVLTAASQETADLVHRTLGRT
                     GIRLPVVSFGVMRSDNPNLCKAAYDRGMRLFDTANGYLGGKSEVMLGNFFRNYPRDSF
                     IISTKVRSSTDRQGRPDSQATSEKFMEFFNVSMGRLKMDYVDILYVHDVSNPEYLEYK
                     PVINTVKKLKQEGRIRYIGFSTHGNEPEVINAAADQDVWDVILTQYNYRLSYIPELNA
                     AIRKAASAGIGIVAMKTLAGGFLDRERTKPVNATAALKWVLQNPDIHTTIPGMTAFDQ
                     VDMNAKIMEDFTLSEQEKNELMASLDDPGLICSGCNQCTADCVKNLPVRDIMRAYMYA
                     YGYGSPAMARNLLAELGTGENPCSECDTCNVRCFRHFDLKERIADISRLHNVPSEFLA
                     "
     gene            complement(5060..5752)
                     /locus_tag="PLZ75_08810"
     CDS             complement(5060..5752)
                     /locus_tag="PLZ75_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS72531.1"
                     /translation="MTSGSISRNPVTSDIIKTSAGDVEMFFIGHGSLMFKVNDFCIHI
                     DPVRSSGSYDNLPKADLILVTHEHYDHLDPELIADLRKEGTLMFSNGTAASEIKWAKP
                     MAAGDVQRAGGIVIEAVPAYNIVNMRSPGQPFHPKGVGIGFILTIGGKRFYIAGDTEN
                     TPEMKALKNIHVAFLPMNLPYTMTPEMVADAARAFKPEILYPYHYGETETGKLVDLLK
                     NTDIEIRIRDLQ"
     gene            5948..>6962
                     /locus_tag="PLZ75_08815"
     CDS             5948..>6962
                     /locus_tag="PLZ75_08815"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HOS72532.1"
                     /translation="MHSGYRKKSLFLLIFLFVTVLIYGQADKSCTISFGGEKQENDIV
                     VSARSISIDYSMPEINIREFKNDQGSFYRISIPGHTQTSETGSPELPVYSKLITIPAG
                     HTYSIRITDVKTEKINPGKKKISGILFPAQESESKAPGIYKSLFSMNKAVYAKREFIP
                     SDTVSIISAGTIRGKSIANLLISPVRYNPHLNTLEAITSMKIEIRFSGAGTASKNYPE
                     SSVFQKTLEKGILNYPKGPDLPAFSEKPLRMVILTDTAFRHHLEPLIQWKTIKGFRID
                     VLYRGAVYAGQTYKEIKNTLTDLYYSSTPENPPPEYLLIVGNTSKIPYYGKDNVTDLY
                     YGEF"
BASE COUNT         1948 a         1638 c         1559 g         1817 t
ORIGIN      
        1 agtcttgaat cataccgtga aacacttaag aatgcagggg aaaaggcaga tattgcacta
       61 ttcattcaga atatgaaaag catgaatctc cggaattcag tgttcatcga ttgcactgcc
      121 aatgaaaagg ttgctgccag gtacacggag atccttgaag cttacatctc ggtggtaacg
      181 gcaaacaaaa ttgcatgctc ttcggattat tcctactatc atcagctgaa aagcactgcc
      241 ggtgaaaggg atgtcagatt catctacgaa accacagtcg gtgccggtct gccaattatc
      301 aaaacgataa atgacctggt actcagcggc gacaagattc tgaaaataga agccgtactt
      361 tccgggaccc tcaatttcat attcaacgaa atgagcggtt ccgtaccgct tagcaaggcc
      421 atacggaagg cccgggaaaa aggttattcg gaaccggacc cccgtattga tctgagcgga
      481 acggatgttg tcaggaaaat cctcatcctt gcacgtgagt caggctataa actggaatac
      541 agtgatgttg tcgtaagcag gtttcttccc gaggaatgct tcagggggga tatcagtgat
      601 ttttacagga aggttgaagc cctggatgat gaatttgaga agaaacgtat tgaccttgag
      661 aaacagaaca ggaagtggag attcacggct actcttgacc atggtaaggc aaaggttgag
      721 cttgcggagg tgggacccgg ccatccctca tttttcctgg aaggcagcaa caatattgtt
      781 ctgctgacca cggaaaggta ttgcgacctg ccaatggtca tcaagggcta cggagccggg
      841 gcagacgtaa ccgcagccgg tgtgtttgcc gatctcatga gggtggtaaa tgtgtaggat
      901 gcaaaccgca tgcctgacag aggccaccgg cggaataaga taaatttaca tcattgaaag
      961 aaagaatcag atattattca accaacctga aagcaccgga agtgtcattc cgggaagctc
     1021 tcatgaaagg cattgcacct gacgggggac tgtacatgcc ccggttcatc ccctctctca
     1081 gtcctgaaga acttatgggt taccggggtc aagattaccc cggaatggcg ttcagctttc
     1141 tctataaatt cctgaaaacg gagattgaat ccgacgtact gaattcactg tgcagggatg
     1201 cctacaactt tgacattccc cttgagccag caggggaaag atcctacata ctccgactgg
     1261 atcagggccc gacggcatcc ttcaaggatt tcgccgcgag gatgatggcc cggctgatga
     1321 accattttat tgccggtgaa aacaggcatt acacgattct cacagcaaca tcgggagata
     1381 caggcagtgc tgttgcaagt gcctttcacg gactgtcaaa tatcaatgtc attattcttt
     1441 tcccgctcgg ggaagtaagt gatacccaga gaaaacagat gaccaccctt cacgggaacg
     1501 tcagagtgat cgggataagg ggaaaatttg acgactgtca gcggattgta aaaaaagcat
     1561 ttctcgacaa gtccctgtcg gatctcaatc tgacttcagc aaattcaatc aatatcggca
     1621 gactgcttcc ccagtcggtc tactacattt atgcctggtc gcggacaggc ttactgccgg
     1681 ttgtattcag tgttccttca gggaattacg gaaacctgat gggaggaatt attgccaggg
     1741 aggctggact gcctgtcaga aagttcatca ttgccacaaa cgagaacaac gaggtaccgg
     1801 aatatcttcg caccggttcg tacagtataa tcgtaccctc ccggaattgt atctcaagtg
     1861 caatgaacgt tggtcatccc agcaaccttg ccagaattgt tgctctttac ggaggatcga
     1921 tgaacgaaca cgggacgatt cttaaatctc ccgacctgga aaggatgaga agggatttcc
     1981 acggcgtttc cgtaactgac gataccacca gggaaaccat tgcgtcatgc cacagggatc
     2041 acggagtact tcttgaaccg cacggggcag tggcatggaa agggcttgaa cactatcttg
     2101 cgaaagccgg tccggaagaa aagaacggga cagtttacat ctccctggaa actgcacatc
     2161 cggcaaagtt tccaggcgaa atagaacgga ttttgagttt tactcccccc gggcatcctt
     2221 cccttgagaa cctggagaaa ctggaagagc attatacacg gatggacaat gattacggat
     2281 cattcacaca gtatgttttc aaacactgtt aatatataaa acatgtacat tatttgtagc
     2341 gaccttgaag gggtacttgt accggaagta tggatcaacg ttgcagaaaa gacggggata
     2401 gacgaactta aactgacaac ccgtgatata aatgattatg acgtgctgat gaaaagaagg
     2461 ctggagatac tcagtcagaa cgggataagc ataggagaca tccggaatgt catttccggc
     2521 cttgaacccc tgcccggcgc actcgacttc atcagctggc tccacggtaa ggcacagctt
     2581 ataatagtat cagacacttt cagggaattt gccgatccta tgcttaaaaa gatgggctgg
     2641 ccgcttctcc tttgccatca ccttacaata gaccggcatg gtatgattac cgattacaat
     2701 ctgcggcaga aagaggcaaa gatgaaagtg gttgaggcgc ttcaaagact gaatttcaag
     2761 gttattgcaa taggtgactc ctacaacgac atccagatgc tgcgcaaggc ggaaacagga
     2821 atacttttca gacctcctca gaatgtaatt gatgacaaca gcgaccttct ggccgtggat
     2881 acctatgatg gactgaagga ggtaatcagc gaaaaacttg aaacagacgg actatcctga
     2941 aacgccggcc tgcagacatg ttgtctgatc caaatgcagc tttccggaat tactgaagtg
     3001 atgtcattac tgttccagta ttctgcttgt atacctgtat gcaatggctg tctgatcctt
     3061 tgccagtcct gatataatca ccatcagatt atccttccac gaaaggaaaa cagtgccatt
     3121 gtaaccgtca ttaaaaacaa atctgttttc atcaccgggt aaagtctcca taccggctgt
     3181 gttcaggtat gattctgcca tatcacgtat ttcccctgca ctttcctttt gtatcagata
     3241 gatactgaaa ctgtcatttc cggattcata cggagccatg aaagcagaat tcagaacact
     3301 gtgtcccaac acattgtcat tgatgtaggt ttcttcattg gcaagtttcc cttcagcggg
     3361 aaatctggtc agcagggccg gaaggctgtt ctcgcccggc agcatgtctg ccaccctgaa
     3421 cgcaagcgtt tcagcagcct gaaggatttt ttccttcccc gaatatgtcc gtattttcac
     3481 atagtagctg cccttgaaaa aattgatggc tccgtcagca ttgtatccct gcgctcccag
     3541 tctgatgaaa ttaaagtggg gtgaacgttc cgatgcatat atgccgaagg ccatcgtatg
     3601 gttttcatgc ctgtaaattt ccagcttaat ggaattcttt ccctttctgt attccgctac
     3661 atgaaggtcc ttaaaaccga gggccaggaa accgtcggca gctccgttaa taaaatccca
     3721 caggttgtca ggcagataga ccggatactc tgttacagtc ctgaatccat gaagatcagg
     3781 aaacacaagg ttctgactac ggacagcaga agaaagaagt ataaaaacaa ggggtaagat
     3841 cagcagtttt ttcatttttc ccggtatatg atttttcagg ccagaaattc ggatggcaca
     3901 ttatgaagcc ttgagatgtc ggcaattctt tctttcaggt caaaatgccg gaagcatctg
     3961 acattgcatg tatcacattc actgcacggg ttttctcctg ttcctagttc tgcaagcagg
     4021 ttcctggcca tggccggact gccgtagccg tatgcataca tgtaggcgcg cataatatcc
     4081 ctgacgggca ggtttttcac acagtcagca gtacactgat tgcatccgga acatatcagc
     4141 cccggatcat ccagcgaagc cattagttcg ttcttttcct gctcggagag tgtaaaatcc
     4201 tccattattt ttgcattcat atccacctga tcaaatgccg tcattcccgg aatggttgta
     4261 tggatgtccg gattctgcaa tacccattta agtgcggctg tcgcattcac gggctttgtt
     4321 ctttccctgt cgaggaatcc gcccgcaagc gttttcattg cgacaatccc gatacctgct
     4381 gaagcagcct tccgtattgc ggcattcagc tcgggaatgt atgaaagccg gtaattgtac
     4441 tgagtcagga taacatccca aacatcctga tccgccgctg catttatcac ttccggctca
     4501 ttaccgtggg tggaaaaacc gatatacctt atcctgcctt cctgcttcag ctttttgact
     4561 gtgtttatta ccggtttata ttccaggtat tccggattgc ttacatcatg aacatatagt
     4621 atatccacgt aatccatttt aagtcttccc atgctgacat taaagaattc cataaacttt
     4681 tcggatgtcg cctggctgtc cggtcgcccc tgcctgtcag ttgaggatct cacttttgtc
     4741 gagataataa aggaatccct ggggtaattc cggaaaaaat tcccgagcat cacctcactc
     4801 ttccccccga ggtaaccgtt ggctgtgtca aacagtcgca tccccctgtc ataggctgct
     4861 ttgcagagat tcggattgtc ggaacgcatt acaccgaaac tgactacagg aagtctgata
     4921 cccgtcctgc cgagtgtcct gtgaacaaga tccgctgttt cctgcgatgc agctgtcaat
     4981 accggagaca ctgcgagggc tccggcacct gttattcctt ttttaaggaa tcctcttctt
     5041 gatattattt ccatgttgtt tattgtaaat ccctgattct tatttcaata tctgtattct
     5101 taagcagatc aacaagtttt cccgtttccg tttccccgta atgataggga tacaatattt
     5161 caggtttgaa ggcccttgcc gcatccgcaa ccatttcagg agtcatggta tagggaaggt
     5221 tcataggcag gaaggcaaca tgtatgtttt ttaaggcctt catttccggc gtgttttcag
     5281 tatcaccggc aatatagaac ctcttgccgc cgattgtaag gataaagcct attccaacgc
     5341 ccttaggatg aaaaggctgt cccggcgatc tcatattaac tatattatat gcggggacag
     5401 cttctataac aatacccccg gccctttgca catctccggc agccattggc tttgcccact
     5461 taatttccga agcagcagta ccgttgctga acataagtgt cccttctttc ctcagatcag
     5521 caatcagctc aggatcaaga tgatcataat gttcgtgggt aacaagtatc aggtctgctt
     5581 tgggcagatt gtcataactg cctgagcttc tgaccgggtc aatatgaatg caaaaatcat
     5641 tcactttgaa catcagcgaa ccatgtccta taaagaacat ttccacatca cctgccgagg
     5701 tttttattat atcggaagta accggattcc gggaaattga acctgaagtc attgtaatta
     5761 aaattaaaat gattaaaata tgtatgcctc ttttcattat tcatggttta ttcgtcatac
     5821 aaaaacatat gtaaaaatag taatctccgg tggtttatca aatttatgcc ttcagagggc
     5881 ggccaaatgg agtaaaatta ataacttgca tctgaatcca ggttaatgaa tcaattaatt
     5941 tacattgatg cattcagggt atagaaaaaa gtcattgttc ctgcttatct ttctgtttgt
     6001 caccgtactt atatatggtc aggccgataa aagctgcact atcagcttcg ggggtgaaaa
     6061 acaggaaaat gatattgtag tatccgcacg ttcgataagc atcgattatt ccatgccgga
     6121 gatcaatatc agggaattta aaaatgatca ggggtcattt tacaggatca gtatacccgg
     6181 gcatacacag acatcagaaa ccggcagtcc cgaacttcct gtatacagca aacttattac
     6241 aatacccgca ggacatacat acagtatcag aatcactgat gtaaaaacgg aaaaaatcaa
     6301 tcccgggaaa aagaaaatca gcgggattct cttcccggcc caggaaagcg aatccaaggc
     6361 tcccgggatt tacaaatccc tgttttcaat gaacaaagcg gtttatgcaa aacgggagtt
     6421 tattccttca gataccgttt ccataatttc agccggtacc atcaggggaa aaagcatagc
     6481 caacctactc atatccccgg tgaggtataa tcctcacctg aacactcttg aggctattac
     6541 atcgatgaag atagagatcc gtttcagcgg agccggaaca gcctcgaaaa actatccgga
     6601 atccagtgta ttccagaaaa ccctggaaaa gggaatcctc aactatccca aaggtcccga
     6661 cctgccggcg ttttcagaaa agcccctgag aatggtcatc ctgaccgata cagcattcag
     6721 acaccatctg gagcccctga tccaatggaa aaccataaag ggattcagga ttgatgtact
     6781 atacagaggt gctgtttatg ccggtcagac ctacaaggaa ataaagaaca cacttactga
     6841 tctgtattat tcctccaccc ccgagaatcc tcctcccgaa taccttctta ttgttggcaa
     6901 cacatcaaag ataccatatt acggaaaaga caacgtaacc gacctttatt acggtgaatt
     6961 tg
//
LOCUS       DAOSPX010000166         6891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10183_length_6891_cov_3.773259, whole genome shotgun sequence.
ACCESSION   DAOSPX010000166 DAOSPX010000000
VERSION     DAOSPX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10183_length_6891_cov_3.773259"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..945
                     /locus_tag="PLZ75_08820"
     CDS             292..945
                     /locus_tag="PLZ75_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72533.1"
                     /translation="MKKINYSAILAVAFLLLASPLFSQQSSDERDFKFTIKTNPLSAL
                     GGPFWIVVVPVTGEYKALFEAAVSKKSSVQIGLGYIGPSILLNLDDLTSDGEGSEIEG
                     IKTSGFRSQGWYKYFLSRDLSAPEGFYIGPHISYASANIKNKRNTSEYVSATKLNING
                     VFGYQLITSGGFTLDIFTGLGFVSRKWEVKGTDWDNDSFKDKASVAVPFGFSFGYAF"
     gene            complement(933..1436)
                     /locus_tag="PLZ75_08825"
     CDS             complement(933..1436)
                     /locus_tag="PLZ75_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOS72534.1"
                     /translation="MISDEIRATVKKIFTEYLERNKHRKTPERYAILDEIYSRTGHFD
                     IESLYISMKNKNYRVSRATLYNTIDLLLDCNLVTRHQFGKNIAEFERAYQCGQHDHLI
                     DLETGTVIEFCDPRIDEIIKTACELNNFRPLYHSLYIYGNISGGMKDPEEPGRKKDTP
                     GSFNQKA"
     assembly_gap    1720..1729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1730..3837)
                     /locus_tag="PLZ75_08830"
     CDS             complement(<1730..3837)
                     /locus_tag="PLZ75_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOS72535.1"
                     /translation="MGKKDFFDEEDKNRIQAEYDSLVNNLLRCNAPEDRELIERAFNT
                     ANEAHWNMRRKSGEPYIIHPIAVAKIVNQEIGLGARSIAAALLHDVVEDTDYTLDDIR
                     RKFNPRIASLIDGLTKISGTYNKPASSSMQAENFRKMLLTLSDDMRVILIKIADRLHN
                     MRTLNSMPEHKKMKIAGETIFLYAPLAQRLGLFSIKSELEDLSFKFRQPQIYEELASK
                     LKHSEKKYYAVINKFSLPIIDKLTSAGYSFDINGRPKSLYSIWKKMQSKAVPFEEIYD
                     VLAVRIVFDPVPEISEKTQCWNIYSMITDTYLPKPDRLRDWISRPKPNGYEALHLTVM
                     GPDGKWVEVQIRSRRMDEVAERGYAAHWKYKGDTSKETELDKWIKKIRVMLENPLEDP
                     IEFLDEFKMNLFSSEIMVFTPKGTLVSLPKGASALDFAYEIHSEIGNKAIGAKINYKL
                     LPLNTTLMSGDQVEIITSDIARPEKEWLSWVRTTRAKEKIKDALKTETINRIQKGIEM
                     LEARLAELEIVPNAEVLKKLMTAYNVSNKDELYSGIELGLINLDNLKRIFKKTESKSI
                     IKYWNFKFIGSKKETGENVHVPEKIDTSAPFILREDVTSAEQSYEIADCCKPIPGDDV
                     TGYRSPEGSIIIHKPTCPTAIRLSSNEGNRIVAVKWAIHKFVSFLARISVEGIDRIGL
                     VNDITNIISYELSVNMTNIN"
     gene            complement(3928..5421)
                     /locus_tag="PLZ75_08835"
     CDS             complement(3928..5421)
                     /locus_tag="PLZ75_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72536.1"
                     /translation="MIRKPVYLTTLILIFLSLNLSGRTDSIPPLPPALERVTVNPESG
                     NVEIEWQTSPSGDVWGYVVYRYIDGAGYAIDTLYGRQITNYLHTGSGASYYSESYVVA
                     AFDSVKNFSKLSRPLGTIYTEVRIDTCNNRLEVKWNSYTPVPYPVTDYSVMMSVDGEP
                     FHKAGETEPENNLFILNEFITERKYCFFVRAGLANGQVSESNSDCVLTRMQKAPAWIN
                     ADYASAGDKGRIKLSFTIDPGSEITTFRLERKTAGENDFRVIKYLTSVSSSVTFTDNE
                     AERETIHVYRLAAVNNCGVPVKYSNTASNIVLRGSFSDNRIDLSWNSYRKWAGIVDSY
                     IISAGTGGEYRQLAVTSPADTLWSVNYPDIMHEISGKEVCFMVTAFEALNPHGLSGES
                     SSFPFCLSLPENITVPNMFIPSGTGVKETNRYFRPVLSFIPADYHLIVTDLKRRVIFE
                     TRDFNEKWDGRLNGAILPEGVYLWMLRIKTPSGTSVSRTGTVTLKNQ"
     gene            5526..>6891
                     /locus_tag="PLZ75_08840"
     CDS             5526..>6891
                     /locus_tag="PLZ75_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOS72537.1"
                     /translation="MLKGRLLRNIFHPLAEKTGQVNLVYLLSLLVGLLSAVAAIILKN
                     GIHYTHQFLLKITAMGSWNLLYLVLPPTGMLLTLLFVKYVVKDNIGHGVSRVLYAISR
                     KKSILNPHKTWTSVVASTITIGFGGSVGAEAPIVLTGSSIGSTIGNFFRLSYRSITLL
                     IGCGAAGAISGIFNAPIAGIVFTIEILMLDLTMSSIVPLLISSVTAATTSYFLMGNEV
                     LFSFSVREAFIITNIPWYLLLGVVSGLLSVYFSKTTLYVESRFERIRNDYVRLAVGGG
                     ILGLLIFLFPPFYGEGYGTIMHLLKGDTNAVFGNTLVGGFQDNFLVIALFMLGLVLFK
                     VFASSATNGGGGVGGIFAPTLFIGGINGYLFSEILNRIFNVNLPDKRFVLVGMAGMMA
                     GVMHAPLTSIFLIAEITGGYDLLIPLIITSTVAFITVKGFEPHSIYHVQLAERGELIT
                     HDKDN"
BASE COUNT         1716 a         1491 c         1553 g         2121 t
ORIGIN      
        1 gataaatctt taaatttata cgtctgtttt tctaaatatg agtcaggtct gaaaaggtta
       61 aaatcagaaa cagggattta atttcacatt atgccgttaa taagttgtgt ttgtaatata
      121 tagtaaatca gaaggacagc aatttgtctg caacattata aaaaacttat agtcagcgag
      181 tcaaacattt cacgtggtgc atgtatattt gaataccaga gaaataatgg ttatgattgt
      241 ttttaagccg aacacaaata agtatttaaa caatttaaca ttctgaagtc aatgaaaaaa
      301 atcaattatt cagcaatttt agcagtagca tttctgcttc tcgcttcacc ccttttttcc
      361 cagcagtcat ctgatgaaag agattttaaa tttacaatta aaacaaatcc gctctctgca
      421 ctgggtggtc ctttctggat agtggtagta ccggtgaccg gtgaatataa agctcttttt
      481 gaagcagctg tgtctaagaa atcatcggta caaatcggtt tgggttatat tggtcccagt
      541 atacttctca accttgatga tctgacctca gatggggaag gttctgaaat tgagggtatt
      601 aaaacaagcg gattcagatc tcagggatgg tataaatatt ttttgagccg ggatctttct
      661 gctcctgaag gattctatat cggacctcac atatcatatg cctccgccaa cattaaaaat
      721 aaaagaaata catcggaata cgtaagtgca accaaactga acattaacgg agtattcgga
      781 taccagttga ttacctcggg tggcttcacc ctggatatct ttaccggcct gggttttgtt
      841 tcaagaaaat gggaagtaaa aggaaccgac tgggacaatg attcatttaa agacaaagcc
      901 agtgtagctg ttccgtttgg atttagtttc ggctatgctt tctgattgaa ggatccggga
      961 gtatccttct tccttccagg ctcttccgga tctttcattc cgccggagat gttaccatag
     1021 atgtaaagtg aatgataaag aggacggaag ttgttcagct cacaggccgt ttttattatt
     1081 tcgtctattc tggggtcgca gaattctatt accgtccctg tttccagatc aattaaatga
     1141 tcatgctgtc cacactgata ggccctttca aattctgcga tgttcttgcc gaactgatgc
     1201 cttgtcacca gattgcagtc aagcagcaga tcaatggtat tgtacagggt ggccctgctt
     1261 accctgtaat tcttgttctt cattgaaata taaagagact cgatatcaaa atgacccgtc
     1321 cttgaatata tttcatccag aatggcatac ctttcaggtg tctttctgtg tttgttcctt
     1381 tccagatatt cagtgaagat cttttttact gttgccctga tctcatcact aatcatattt
     1441 ggtctaaatt aaaataatag caaaaatata caatttttgt ttaataccaa ggcagggaaa
     1501 gaggaggctt tgttgtgaag tgaagtgtga ttctgttgtc actgccgtta ccgggatcac
     1561 atgcagggga ggggagtttt tgtcatgaaa aatcttcgac ccgtttgaca ctctcaatgc
     1621 ctttcacatt tgatattttg agtatgaggt tgttaaggtc ttttgtgtga tgtacataca
     1681 gatcgatggt gccttcaaag atacccttgt tcacagagan nnnnnnnnna agttaatatt
     1741 tgtcatgttg acgctcagtt cataggaaat gatattggtt atatcattta caagtccgat
     1801 tctgtcaatt ccctccacgc ttattctggc aagaaaggac acgaatttat ggatggccca
     1861 tttaacggcc actatcctgt taccctcgtt tgagctcagg cggatggctg tagggcaggt
     1921 tggtttatgg atgatgatgg aaccttcagg cgatctgtag cctgtaacat catctccggg
     1981 tatgggtttg cagcagtctg ctatttcata tgactgttcg gcgcttgtta catcctccct
     2041 gagaatgaag ggtgcactgg tatctatttt ttccggaaca tgaacatttt cccctgtttc
     2101 cttttttgat cctataaatt tgaagttcca gtatttgata atactttttg attccgtttt
     2161 tttgaatatc cttttaagat tgtcaagatt tatcagtccc agttcaattc ctgaatagag
     2221 ttcatcttta ttggatacat tataggcagt catcagtttt ttgaggactt ccgcattcgg
     2281 aacgatttcc agttcagcaa gcctggcctc gagcatctct atgcctttct ggatgcggtt
     2341 tattgtttcg gttttgaggg catcctttat tttctccttt gcccttgttg ttctgaccca
     2401 ggaaagccat tccttttccg gccgggcaat atcggatgta attatctcca cctggtcgcc
     2461 gctcataagg gttgtgttca ggggaagaag cttgtagttg attttggctc cgattgcctt
     2521 gtttcctatt tctgaatgga tttcataggc gaaatcaagt gcggatgcac ctttgggaag
     2581 gcttaccagt gtgcccttgg gtgtgaacac cattatttcg gatgaaaaga gattcatttt
     2641 gaattcatcc aggaattcaa tcggatcttc gagaggattc tcgagcatca ccctgatctt
     2701 ttttatccat ttgtccagtt ctgtttcctt tgaagtatct cccttgtatt tccagtgagc
     2761 ggcatatcct ctttccgcca cttcatccat ccgcctgctc cgtatctgga cttccaccca
     2821 ttttccgtcg ggacccatta ccgtaaggtg cagtgcctca tagccgtttg gcttcggccg
     2881 gctgatccag tcgcggagtc tgtcaggctt tggcaggtaa gtgtccgtga tcattgaata
     2941 aatattccag cactgggttt tttcagatat ttccggaacg ggatcgaaga ctatccttac
     3001 tgctagaaca tcatatattt cctcaaaggg cacagccttg gactgcatct tcttccagat
     3061 ggaatagagc gatttcgggc gtccgtttat atcgaaactg tatcctgccg aggtcagttt
     3121 gtcgattatg ggaagggaaa atttgttaat aacggcgtaa tactttttct cactgtgttt
     3181 cagtttcgag gccagttctt catagatctg cggctgccgg aacttgaaac tcaggtcctc
     3241 aagttcgctt tttattgaaa atagtccgag acgctgggca agaggagcat aaaggaatat
     3301 tgtctcaccg gctatcttca ttttcttgtg ttcgggcatt gaattgagag ttcgcatatt
     3361 atgaaggcgg tcagcaatct tgataagtat cactctcatg tcgtccgaaa gcgtaagcag
     3421 cattttccgg aaattctctg cctgcattga ggatgaggcc ggcttattgt aagtacctga
     3481 tatttttgta aggccgtcta tcagcgaggc tatcctggga ttgaattttc tccttatgtc
     3541 gtcgagggtg taatccgtat cctcaaccac gtcatgaagc agggctgcgg caattgacct
     3601 tgcaccaaga cctatttcct ggtttactat ctttgccacg gctatgggat gaataatgta
     3661 tggttcacct gatttccgac gcatattcca gtgagcctca ttagccgtat tgaaagccct
     3721 ctcaataagt tccctgtcct ccggagcatt gcatctcagg agattattaa caagtgaatc
     3781 atattcggcc tgaatacggt ttttatcctc ttcgtcaaaa aaatcctttt tccccataaa
     3841 tttatactgt actttgcgcg gaggatacaa atatagcttt attcatttaa aaacggatga
     3901 ttaaggcctg aatgataaac tccggaccta ctgattcttc aatgttaccg ttccggttct
     3961 cgacactgat gtgcccgacg gggtctttat tctgagcatc cacaggtata ctccttccgg
     4021 aaggatcgcg ccgttgagcc ttccgtccca tttttcgttg aaatcgcggg tttcaaagat
     4081 gacccttcgc ttcaggtcag tgacaatgag atgataatca gcgggtatga atgacagaac
     4141 cggacggaag tatctgtttg tttccttcac gccggtgccc gagggaatga acatattcgg
     4201 gacagtgata ttttccggca gcgacaggca gaaggggaag gaagaacttt cacctgacag
     4261 tccgtgggga ttgagggctt caaatgccgt aaccatgaaa cacacttcct ttccgcttat
     4321 ctcatgcata atgtcaggat aatttaccga ccagagcgta tcagccgggg atgttaccgc
     4381 cagttgcctg tattccccgc cggtaccggc agagataata tatgaatcca ctataccggc
     4441 ccacttcctg tatgaattcc agctcaggtc aatcctgttg tctgaaaagc ttcccctgag
     4501 cacaatgttt gaggcagtat tggaatattt caccggaact ccgcagttgt tgactgcggc
     4561 cagcctgtaa acatggattg tctccctctc agcctcatta tcggtgaagg taactgatga
     4621 actgactgaa gtcagatatt ttatgacccg gaaatcattt tctcccgctg ttttcctttc
     4681 aagcctgaat gttgtgattt ccgatcccgg atctattgtg aatgacagtt ttattctgcc
     4741 cttatcgccc gcgcttgcat aatcggcatt gatccatgcg ggagccttct gcatccgggt
     4801 caggacacag tcgctgtttg actcggaaac ctgtccgttt gccaggcctg cacgaacaaa
     4861 gaaacagtat tttctttctg ttatgaattc atttaaaatg aataagttat tctccggttc
     4921 cgtttcgccg gccttatgga aaggctctcc gtccactgac atcattactg aataatcagt
     4981 tacagggtag ggaacaggcg tgtagctgtt ccattttact tccagcctgt tgttgcaggt
     5041 gtcaatacgg acttccgtgt atatggtgcc caggggtctg gaaagcttac tgaagttctt
     5101 cactgaatca aaggcagcaa caacatagga ttcactgtaa taggatgcgc ctgatcctgt
     5161 atgcaggtaa tttgtgattt gccggccata aagggtatca atggcatagc ctgccccgtc
     5221 aatatagcgg tagaccacat atccccacac gtcgccggaa gggcttgtct gccattctat
     5281 ttccacattt ccgctttcag gatttaccgt gacacgttca agtgccggag gcagcggagg
     5341 gatggaatct gtccggcccg ataaattcag ggaaaggaaa atgaggatca gggtagtaag
     5401 atatactggt tttcttatca tgtcggagcc ttcgggataa ccggtctgag ttttttttca
     5461 gcctcctgta caaacttaat taaaaaacgg agacgcttta ggaatgtttt tatatttgca
     5521 aaattatgtt aaagggcagg ttgttaagga atatttttca tcctctggca gaaaaaacag
     5581 ggcaggttaa ccttgtttat ctgctaagtc tgctggtggg cctgctcagt gccgtggctg
     5641 ccattatact caagaacgga attcattata ctcatcagtt ccttctgaag ataaccgcca
     5701 tgggttcatg gaatcttctt tacctggtcc tgccgcccac cggcatgctt ctgaccctgc
     5761 tctttgtgaa atatgtggtg aaggacaata tagggcacgg ggtaagcagg gttctgtacg
     5821 ccatatcaag aaagaagagc attcttaatc cgcacaagac ctggacatcc gttgtggcat
     5881 caaccataac catcggattc gggggctcag tgggagcaga ggcacccata gtgctgaccg
     5941 gttcatcaat cggatccaca ataggtaact ttttcaggct cagctacaga agcatcacgc
     6001 ttctgatagg atgcggtgcc gcgggtgcca tatccggaat tttcaacgca cccattgccg
     6061 gtatagtgtt tacaattgaa atactgatgc ttgaccttac gatgtcatca attgttcctt
     6121 tgctgatatc ttcggtcact gccgcaacca ccagctactt cctgatgggg aatgaggttc
     6181 ttttttcatt ttccgttaga gaggctttca tcattaccaa cataccctgg tatttgctgc
     6241 ttggggtggt atccggcctt ttgtcggtat acttctcaaa aacaacactg tatgttgaaa
     6301 gccgcttcga aaggatcagg aatgactatg taagactggc agttggcggc ggaattctcg
     6361 gactgcttat atttttgttt ccccctttct acggggaggg ttacggaacc ataatgcacc
     6421 tgctgaaggg ggatacaaat gcagtattcg gaaacacact ggttggcggg ttccaggata
     6481 actttcttgt tattgccctg ttcatgcttg gccttgtcct tttcaaggtg tttgcatcga
     6541 gtgcaaccaa cggaggagga ggcgtgggag gtatttttgc ccctacactg tttatcggag
     6601 gcataaacgg ataccttttt tctgaaatac tgaaccggat attcaatgtg aacctgcctg
     6661 acaagagatt tgtccttgtg ggaatggcag gcatgatggc aggcgtgatg catgcacccc
     6721 tgacatcaat tttcctgatt gcggaaataa ccgggggata tgatctgctg atacccctta
     6781 tcattacctc aaccgtggca tttataacgg tgaagggctt tgagccccat tcaatctatc
     6841 atgttcagct ggcggagagg ggggagctga tcacccacga caaggacaat g
//
LOCUS       DAOSPX010000167         6850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10268_length_6850_cov_4.204268, whole genome shotgun sequence.
ACCESSION   DAOSPX010000167 DAOSPX010000000
VERSION     DAOSPX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10268_length_6850_cov_4.204268"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PLZ75_08845"
     CDS             <1..1157
                     /locus_tag="PLZ75_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS72538.1"
                     /translation="GHAIRQTAGPQHRMLSFLPLCHAYERTMNYEFQQLGISIYYAES
                     LSTIARDLASCHSDGFSAVPRVLEMMYSKLEEAGKNLKGIKRLIYTWAWNFANSYDNK
                     NRAYIYRLRHNLADRLVYRKWRDNLGGHEMWVVSGGAAIRPNIIRLFTAARLNLYQGY
                     GMTETSPVITVNSPADNELGTNGTPLDDTELIIASDGEILVKGPHVMKGYYKDEKGTR
                     EIIDEDGWLHTGDIGYLKDGKYLMITDRKKEIFKLSSGKYIAPQMIENRLRESSFIDN
                     CLVFGENQKFASALIIPDMKKVREWCRKNGIRADEGDDKMISNRKVVEMLNREVAVVN
                     RTLSDYEIIKKSFYITGSWTVENGMLSQTLKPRRALIMEKYRSIAESAYK"
     gene            complement(1415..3091)
                     /locus_tag="PLZ75_08850"
     CDS             complement(1415..3091)
                     /locus_tag="PLZ75_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HOS72539.1"
                     /translation="MEWLEALFFNDGIARVILLYSLAIASGVLLGKIKIWGVSPGPTF
                     VLFTGLLIGHLGFTVSREVSDFIREFGLILFIYSIGLQVGPGFFSSFRKGGMQLNLMA
                     AGVVIMGVAVTISLYYLLGGRIPMPMLVGIMSGAITNTPGLGAAQEALRQAYEAGQIA
                     EIPQIALGYAAAYPLGVIGIILSIILIRVIFRVSLEDEERKLEKEKESSWDRLEIFTL
                     RLTNKSISGRELYEINKLIGRNFVISRLKRDDLFSVPKTNTVLLEGDIVLVVAATADV
                     EPITAFIGEAAETDWKVSEPALESRRIILTRSEINGRTLGSLRLRTIYGVNVTRVNRS
                     GVDLLGSANLVLQVGDAVTVVGEVDALEKVEQFLGNTLKRLNEPHIYTIFIGIFFGII
                     FGSIPFYIPGIPMPVKLGLAGGPLIVSIIIGRFGYKFRLVTYTTQSANLMLREIGITL
                     FLASVGIASGGQFAATVLSIDGLVWIAAGFLITTLPLLIAGIIGMKFLRLNYFSLAGL
                     MAGSTTDPPALAYAGSIAGNDEPAISYSTVYPLTMFLRVFMAQILILLFL"
     gene            3403..4839
                     /locus_tag="PLZ75_08855"
     CDS             3403..4839
                     /locus_tag="PLZ75_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72540.1"
                     /translation="MATEKRRAFIRKSAMATAGITLGAPAFIRDYAKIQPSDILNVGV
                     IGINDRGGFYGGSGHTASFTKIRETRVTAVCDCVEYLLPKAVKDVENLGGAKPATYID
                     YRRMLENKDLDIVSIATPDYWHALMTINACQTGRDVYVEKPLAYNIDEGRKMVQAARK
                     YNRIVQIGTQRRASRINQKAIQMLRDGVIGDVYMGRATVFRSRPSIGRKPDGPAPDGV
                     NWDLFRGPAPMIPFNENHFLYNWHWYWDTSTSEFGNNGTHFIDLLRLGMNISEHPVKA
                     AACGGFYAYQDESDQDVPNFQVATFEFANNSVIELEVRSLPTPNEPWGFIWFGTKGYA
                     YISGNSFLVFLGDGKPASVGGQTGTAAFSTGLQQDRSTVPSIIVDQSDLDPDPRREEI
                     AKAGIDFHFQNFVDCVKSRNRADLLADVEEGHISTTMMHIGNTAYLTGRKLIFDGRAE
                     RYVNDDVANSYLSRPGGGRKPYNVPDRV"
     gene            complement(4984..6450)
                     /gene="aspA"
                     /locus_tag="PLZ75_08860"
     CDS             complement(4984..6450)
                     /gene="aspA"
                     /locus_tag="PLZ75_08860"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOS72541.1"
                     /translation="MRNANTRILGYSAQYESGRTRWEHDLLGEREVPYEAYYGIQTMR
                     AVENFNISGVTLNFYPSLINALAMVKESAAKANFELGLLDKNVRDAIVKACRELRNGK
                     YHNQFVVDMIQGGAGTSTNMNANEVIANRALEILGHERGEYVHCHPNNHVNLSQSTND
                     AYPTAAKIALFNSNKNLTAVLSGLIDSFRKKGLEFSHIIKMGRTQLQDAVPMTLGQEF
                     EAYAVTLEEEIERLNQNAALFLEVNMGATAIGTGINSAPGYSEKVVSYLRRITGLNLV
                     LASNLVEATQDTGVFVIYSSAVKRLALKLSKICNDLRLLSSGPRTGINEINLPPMQPG
                     SSIMPGKVNPVIPEVVNQIAFKVTGNDLTVTMASEGGQLELNVFEPVIVQSVFESVEM
                     LINGMNTLKHRCIDGITANEERCRSLVHNSIGLVTALVPWLGYEVSNALAREALETGE
                     SVYSLVLKKNLLSTEDLDTILSPENMIRPGKMKADTPE"
     gene            complement(6574..6696)
                     /locus_tag="PLZ75_08865"
                     /pseudo
     CDS             complement(6574..6696)
                     /locus_tag="PLZ75_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1806 a         1526 c         1690 g         1828 t
ORIGIN      
        1 acggtcatgc aatccggcag acagccggtc cgcagcacag gatgctgagc tttcttcccc
       61 tgtgccatgc ctatgaaagg accatgaatt acgaattcca gcagcttgga ataagtattt
      121 attatgccga gagcctgtca accattgcaa gggacctggc aagctgccac agcgacggat
      181 tcagtgcggt gccaagggtg cttgaaatga tgtattccaa gctggaggag gcagggaaaa
      241 acctgaaggg gataaaaaga ctgatttaca cttgggcatg gaattttgca aacagctacg
      301 acaataaaaa cagggcatat atttatcgtt taaggcataa tctggctgac aggcttgttt
      361 acaggaagtg gcgtgacaat ctgggaggcc atgaaatgtg ggtggtgtcg ggaggggctg
      421 caatacggcc caatatcatt cgtttgttca cagctgccag actaaatctt taccagggtt
      481 acggaatgac agaaacatct ccggtgataa cggtaaattc accggccgac aacgagcttg
      541 gtaccaacgg aacccctctt gacgatacgg agcttataat tgcctctgat ggtgaaatac
      601 ttgtaaaagg gcctcatgtg atgaagggat attacaagga cgaaaagggc acacgtgaaa
      661 ttattgatga ggatggatgg ctgcataccg gggatatagg ctatctgaaa gacggaaaat
      721 acctgatgat aaccgacagg aaaaaggaga tttttaaact ttcatcaggc aaatacatag
      781 caccacagat gatagagaac aggctgcgtg agtcttcttt tatcgataac tgcctggttt
      841 tcggggaaaa tcagaaattt gcctctgccc tgatcattcc ggatatgaaa aaggttcgtg
      901 aatggtgcag gaaaaacgga atcagggctg atgagggtga cgataagatg ataagcaaca
      961 ggaaagttgt tgaaatgctt aacagggaag tggccgttgt caacaggact ctttcagatt
     1021 acgaaataat aaaaaagagt ttttatatca ccgggtcctg gacagtggaa aatggtatgc
     1081 tctcccagac cctgaagccc aggagggcgc ttatcatgga aaaatacaga tccatagctg
     1141 aatcagcgta taaataaggg gaacaggtaa aagtttcagt gttttatgga acctgactgc
     1201 tgatacctgg caggttattc tgatctgctt gtgatatgcc agcacccgca tacagaacat
     1261 ttgtaggccc ggagattttt tacaaaacgg atttccttta tatgccggat catctcttct
     1321 gcttctgcgg cggaattgta tatcttcttc ctgcacggag gagtaactgg ttttacattt
     1381 ttgatatttg aggctccggc tgccaaattc agttttataa aaataaaagt atcaggatct
     1441 gggccatgaa aacacgaagg aacatggtta aggggtaaac cgttgaatag gatattgcag
     1501 gttcgtcatt gccggcaatt gaaccggcat atgccagagc gggtggatca gttgtgctgc
     1561 ctgccatgag cccggccagc gagaaataat tgagccttaa aaatttcata ccgataattc
     1621 ccgctatcag aagcgggagg gtggttatca aaaagcctgc tgcaatccag acaaggccgt
     1681 caattgataa tactgttgcg gcaaattgcc cgcctgaagc aattcccaca ctggcaagaa
     1741 acagtgtgat accgatttca cggagcatca ggtttgcact ctgggttgta taggtcacca
     1801 gccggaattt gtatccgaag cgtcctataa ttattgatac gatgaggggg ccgcctgcca
     1861 gacccagttt gaccggcatg ggaattccgg gaatataaaa gggaatgctt ccgaaaataa
     1921 tcccgaaaaa tatcccgatg aaaattgtgt aaatatgtgg ttcattaagc cgcttcaggg
     1981 tgtttcccag gaactgctcc actttttcca gtgcatcaac ctcgccgacc acggttacgg
     2041 cgtcgccgac ctgaaggacc aggttggcag agcccagcag atcaactccg gaacggttga
     2101 cccgggtgac atttacacca taaatggtac gaagcctgag tgatccgagt gtcctgccat
     2161 ttatttcact ccgggttaat atgatgcgcc gtgattccag ggccggttcg gataccttcc
     2221 agtcagtttc tgccgcctcg cctataaagg ctgttatggg ttcaacgtcg gcggtggctg
     2281 caacaacaag aacaatatct ccttcaagca gcactgtatt ggttttggga acggaaaaca
     2341 gatcatcgcg tttcagacgt gaaatgacga aattccgccc tatcagttta tttatttcgt
     2401 acagttccct gccgctgatt gatttgttgg tcagcctgag cgtgaatatt tcgagcctat
     2461 cccaggatga ttctttctct ttttcaagtt tcctttcctc atcttcaagg ctgacacgga
     2521 aaatcactct tatcagtatt attgacagga ttattccgat cactcccagg ggataggctg
     2581 ctgcatatcc cagggctatc tgtggtattt ctgcaatctg tccggcttca tatgcctggc
     2641 ggagagcctc ctgtgcggca ccaagccccg gtgtgtttgt tattgcaccc gacattatcc
     2701 ccacaagcat cggcatcggt attcttcctc cgagcagata atacagtgaa atggtcacag
     2761 caacacccat gatcaccaca cctgccgcca tcaggttcag ctgcatgcct cccttcctga
     2821 aggaggagaa aaaaccggga cctacctgaa ggccgattga ataaataaaa aggatcagtc
     2881 cgaattcccg tatgaaatcc gaaacctccc ggctgaccgt gaaaccgagg tggcctatca
     2941 gaaggccggt gaagaggacg aatgtgggcc cgggcgatac gccccagatc tttattttcc
     3001 cgagaagaac accgctggca atggccaggg agtagagcag gataaccctg gctattccgt
     3061 cattgaaaaa taatgcttcc aaccattcca tgcattcgga tatttatttc ccgggcgcct
     3121 tgcgccgcct tatttcctga aagattccct cctgtatgtt ttcagattgt ctttcgggct
     3181 tatttacgga ttttaaaccg tatttaccgg ccacaaattt aaaaaatcag gacggattat
     3241 ttaatgatct tgatcagtag agtattaaac cggttagttt cggcaatctt cctgaatata
     3301 ttaattgccg gtatatccgt taagttaatc tatttattat ttttaacaaa tctcatgaac
     3361 cggaaatatt tttgaaacaa ttaaatatct ggaggaaaaa taatggcaac tgaaaaaaga
     3421 cgtgcattta tcaggaagtc agctatggct acggcaggca ttaccctggg agcgcccgca
     3481 tttatcaggg attatgcgaa gattcagccc agtgatattc tgaatgtggg agtaatcgga
     3541 ataaatgacc gtggaggttt ttacgggggg tccggccaca cagcgagttt cacaaagatc
     3601 agggaaacca gggtaacggc tgtatgtgat tgtgttgaat accttctgcc caaagccgta
     3661 aaggacgttg aaaatcttgg tggtgcaaaa cccgccactt acattgatta ccgcaggatg
     3721 cttgaaaaca aggatcttga tatagtatcc attgccacac ccgattactg gcatgccctg
     3781 atgaccatta atgcatgtca gactggcagg gacgtatatg ttgaaaaacc tcttgcatac
     3841 aatattgatg agggaaggaa gatggttcag gctgcaagaa aatataacag gatagtccag
     3901 atcgggactc agagaagggc cagcaggata aaccagaaag ccattcagat gcttcgtgat
     3961 ggtgtgatag gtgatgttta tatggggagg gcaacagtat tccgttcacg gccaagtata
     4021 ggcagaaagc ctgacggccc tgccccggac ggtgtgaact gggatctttt cagaggccct
     4081 gcacctatga taccgttcaa tgaaaaccat ttcctttata actggcactg gtactgggat
     4141 acaagtacaa gtgaattcgg aaacaacgga acgcatttca ttgaccttct cagactggga
     4201 atgaatatca gtgagcatcc ggtaaaggca gcagcctgcg ggggatttta tgcctatcag
     4261 gacgaatcgg accaggatgt gcccaacttc caggtagcca ccttcgaatt tgcaaacaat
     4321 tccgttattg aacttgaagt cagaagcctg cccaccccga atgagccctg gggctttatc
     4381 tggttcggca caaagggtta tgcatatatt tcaggaaaca gtttcctcgt cttcctgggt
     4441 gatggtaagc cagcctcagt cggcggccag acaggaactg ccgctttttc aaccggattg
     4501 cagcaggaca ggtcaactgt gccaagtatc atagtggacc agtcagatct tgatcctgat
     4561 cccaggcggg aggagattgc aaaagccgga attgattttc atttccagaa ttttgttgat
     4621 tgtgtgaaaa gccggaacag ggctgatctg cttgccgatg tggaagaggg acatatttca
     4681 accaccatga tgcatattgg aaataccgcc tacctgacgg gaaggaagct gatatttgac
     4741 ggcagggcgg aaagatatgt gaatgatgac gtggcaaatt cctatttgtc acgtccggga
     4801 ggtggcagaa aaccctacaa tgttcctgac agggtctgaa cagtattcat tatctatcag
     4861 tgtccgggat cagcgtcggg gttccgtagt aaaagctgtt tctgacattc tgtactgatt
     4921 atttcttgtt ttcagcatat tgtatcaaca aacaattaat ccggctttgc ggcagggagc
     4981 tttctattcc ggagtgtcgg ctttcatttt tcccggccgt atcatatttt ccggggaaag
     5041 gatggtatcc aggtcttcag tggataaaag attcttcttg aggaccaggg aataaacact
     5101 ttcacccgtt tcaagggctt ccctggcaag ggcattggag acttcatagc ccagccaggg
     5161 aaccagggct gttaccagac cgatactgtt atgtaccagt gatctgcatc tctcttcatt
     5221 ggccgttata ccgtcaatgc accggtgctt cagggtattc attccgttta tgagcatttc
     5281 aacggattca aatacgctct ggacaattac cggttcaaag acattcagtt caagctgacc
     5341 tccttcggag gccattgtta cggtcaggtc atttccggta accttgaatg ctatctggtt
     5401 gaccacctcc gggatgacag gattcacctt tcccggcatg atagacgatc cgggttgcat
     5461 cggcgggaga tttatttcgt ttattcctgt cctggggcct gaggacagta agcgaagatc
     5521 gttgcatatt ttggaaagct tgagggccag ccgtttcact gccgatgaat agattacgaa
     5581 aacacctgtg tcctgggtgg cttcaaccag gtttgatgca agcacaagat ttagtccggt
     5641 aatccttctg aggtagctta ccactttttc tgaatatccc ggtgcggaat ttattcccgt
     5701 acctatggca gtggcaccca tgttgacttc aaggaataat gcggcattct ggttgagtct
     5761 ttcaatttcc tcctcaaggg ttacggcata ggcttcaaat tcctgtccca gcgtcatggg
     5821 aacagcatcc tgaagctggg ttcttcccat cttgataata tgggagaact ccagtccctt
     5881 tttcctgaat gagtcaataa ggccgctcag gacagcggta agatttttgt tggaattgaa
     5941 aagggcaatt tttgctgccg tgggataggc atcatttgtt gactgtgaaa ggtttacatg
     6001 attgttggga tggcagtgga catattctcc cctttcatgg cccagtattt ccagtgcccg
     6061 gtttgcaata acctcattgg cattcatatt tgttgaggtc ccggctcctc cctgtatcat
     6121 atccaccaca aactggttat ggtatttccc gtttcgcagt tccctgcatg cttttacgat
     6181 tgcatccctc acatttttgt caaggagtcc gagttcaaaa ttggccttgg ctgctgattc
     6241 cttcaccatt gccagggcat tgataagtga gggataaaag ttcagtgtca ctccggaaat
     6301 attgaagttt tccacagccc tcatggtctg tattccataa tatgcctcat agggaacttc
     6361 gcgttcgccc aggaggtcat gttcccagcg tgtcctgcct gattcgtact gtgcggaata
     6421 tcccagtatc ctggtatttg cgttccgcat tctcctggat atgatgcggg tgatgtttga
     6481 caggattttg agggtggttt ccgcacggta attaaaaaat ttccggttga cattcagcag
     6541 gattgtaggc attacagtcc gggcatgggt ggagtgaagt gatccctggt cccagattcc
     6601 ctcacccaga aaatccccgg tctcaaacag tgcaagcctg acttccgtac cgtaggtgtt
     6661 aagcttgaaa agttccacct caccgtcata gatgatgaat atgtcatcgc ggggactgtt
     6721 ttcgtaaaac aggatttctc cggcctcgca tgacttggtt attatattgt cggcaagctc
     6781 ccggatttcc gtgtcgttaa ggtttctgaa gagttctatt cccttcagaa aaagcgggat
     6841 atgatttttg
//
LOCUS       DAOSPX010000168         6800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10359_length_6800_cov_3.496071, whole genome shotgun sequence.
ACCESSION   DAOSPX010000168 DAOSPX010000000
VERSION     DAOSPX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10359_length_6800_cov_3.496071"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLZ75_08870"
     CDS             <1..594
                     /locus_tag="PLZ75_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS72542.1"
                     /translation="RGIIIGTRPDCIDEEKLGLLRELARTHYVAVEYGIESCYDSTLR
                     RINRAHTFEDAVNAVKMTSAYGISTGAHFIFGFPGESREEMLAEADIISVLPLTTVKF
                     HQLQIIKGTMIEKEYLADPSKFQLFSREEYIDFIVSFLERFNPEIVVERFSGEAPPRF
                     LAVSLWGKKRSDQVSAMIERRLEELDTWQGRLYKKHT"
     gene            967..4335
                     /locus_tag="PLZ75_08875"
     CDS             967..4335
                     /locus_tag="PLZ75_08875"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOS72543.1"
                     /translation="MAIRTEFISGLNRIFRSLMIPAFTAFYVSILTAQVSVSEEEWVI
                     PTYLVAPSNKNPIFYNNETHQGAARHVYPYAMNDTYLNRRVDKAYKALILENEFIKLC
                     VTPEIGGKLYYATDKTNGYNFIYKNDVVRPSNIGMLGAWVSGGIEWCVLHHHRASTFL
                     PMDYEMVENADGSKTIYIGETEPRHRIRWTIGITSFPGKSYFRAELKIHNPTPFNHSF
                     LYWANVAAHTNKDLQVIFPPSVQFATYHAKNDFTHWPLSTEVYQGQDFTKGVDISWWI
                     NSVNANSYFAHDLKEDFMGAYDHGRQSGTVHIGDHNIVKGAKLWEWGSGPRGQATEAR
                     LTENAGPYVEIMVGAYSDNQPDYSWIKPYEVKISEQYWYPVKDIQGFKNANLNGAVNL
                     ESRQKNSVFLGYYSTSKVNKARIILKNRDKIIFEKLTEISPVTTFTRTVKIDGPFSLT
                     DLYTEMTDTETGEVLVSYQPRENEYQEKLPDIVEAPPAPKDIATVEEVYLTGKRLEQF
                     HNSRYDPMDYYEEALSRDPGDIRTNTAVGNICLKNGDYINARKYLGRAIKRLTKDYTR
                     PMDCEALYLQGLTLKALELYDEAVDTLYRAAWDYPFHAASYLELARISVSGGDLEKAL
                     HQINESLASNARNNSSISLKASILRKRGDYIAAAAIVEPLLQNDPLDFRAANESYLLA
                     KESGNREKADRELASLNRKMRNSDQNYLETAIGYINDGLRDEAEDLLLRFNGKNPIIS
                     YYLAYLADKRGDKTLAEKYFKEGSAQSEDYCFPFRLETIKVLETALKYNPADGKAHYY
                     LGNIFYHRNQRDKAIAQWETAVGHSPGLAVAHRNLGWAFYNHLGDGFKAISAYEKAIE
                     LRRDEAIYYEELDHLYEMSNASIARRLKLFEGSNDVVSGRDDAFARQIAVLTLGGQAA
                     EAVEYLSGKEFSYREGSGRVRDIIIDAHLMLGLKYMEDKDYRKALDNFSLAQVPEEEA
                     SGSRAGNRNIQVYYFLGLANEALGNKSKARNYFASCAGLSSSPSTYISYYRGMSNLKL
                     GKKNEALTIFNSMLAEADRQLSQPEAPDDFFAKFGQREAENARLSNIYLLKGLACKGL
                     ADTEPAAENLSRAVDLSESNLWARSELNRLDSTY"
     gene            4768..6036
                     /locus_tag="PLZ75_08880"
     CDS             4768..6036
                     /locus_tag="PLZ75_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOS72544.1"
                     /translation="MTIKAESTSGDKKPVGCGAVAMGQIMWYWQYPASYNWDIIPTAL
                     HEGDDAEPAAELARLLRDCGSVARMNYCCRGSWATTENVEQGLKDLGFPGATKRVRSS
                     WPSSSGWQNLIRAELDAGRPVLYRGGAVIDPGDWGNVHYFVCDGYDRNDPSYFHFNWG
                     WGDTPLSDPDYGYCFTLANLAPDGNNNYTSYQQMIIGISPGCAIQGSITSLPYTSIST
                     PVSLNANTISIPGAGSILEIEPGGNLSMSATNSITLNPGFFAKAGSVVTASVGPYECE
                     CDDISVRGWTNFFACDNCGKQLYNSYQVLSYGSPSLMLSSPEPEGVLINETGIETNKG
                     ENENKADELQQEDKNILLVPNPNEGRFQLRTVFDNYRVQIFNPSGNLIYDKSSRRPTI
                     DIDLSRYPQGTYFMRLTTGGNVYTSTILKQ"
BASE COUNT         2044 a         1347 c         1586 g         1823 t
ORIGIN      
        1 aggggtatta tcataggaac aaggccagac tgcattgatg aggaaaagct cggactgctc
       61 agggaattgg cccgcacaca ttatgtggct gtcgaatacg ggattgaaag ctgctacgac
      121 agtacccttc gcaggataaa ccgtgctcac acatttgaag atgcggtgaa tgctgtgaag
      181 atgacatcag cttatggtat cagtacggga gctcatttta ttttcggatt tccgggagag
      241 agcagggagg agatgctggc agaggcagat ataatttcag tcctgccgct taccacagtc
      301 aaattccatc agttgcagat cataaaagga acaatgattg aaaaggaata cctggctgat
      361 ccttcgaagt ttcagctttt cagtagggaa gaatatattg attttatagt cagcttcctg
      421 gagagattta atccggagat tgtggtggag cgcttcagcg gtgaagcccc tccgcgtttc
      481 ctggctgtgt cattatgggg gaaaaagcgc agtgaccagg taagcgcaat gatagaaagg
      541 agactagaag aacttgatac ctggcagggg aggttgtata aaaaacatac ctgacataaa
      601 aaggcttttc ggtttggagc attggctgaa agcctgatat ctctcggtca gaatgatttg
      661 ttctgcttct gattctgttc ctggatgatc ccttgacttt ctgacttgct ggttgaaagg
      721 tccccggtcc ttctgacttg ataactggat attctgtact tcccctgatt cggtgccgtg
      781 atgatccctt gattttccga cttggaggct tgctgaacgg ttctccatct gttgcgggct
      841 ggatggccag gacagaaaag agcagcggta ttaattcaat aatttgagat gataaagaat
      901 tttagtactt tttaattctt gaatatgatg ctcatattaa cagataatta acttaaaatc
      961 attcttatgg ctatcaggac agaatttatt tccgggctga acaggatttt caggtcattg
     1021 atgattcctg ccttcactgc attttatgta agtattctca ctgcacaggt tagcgtttca
     1081 gaggaggaat gggtgattcc aacctatctg gtggctccgt ccaacaagaa tcccattttt
     1141 tataacaatg agacccatca gggtgctgcc aggcatgttt atccctatgc aatgaatgat
     1201 acttacctga accggagggt tgacaaggca tataaagccc tgatcctcga gaatgagttt
     1261 attaagttat gtgtcactcc cgaaatagga ggcaaattgt attatgccac ggacaagacc
     1321 aatggatata attttatcta taaaaatgat gttgtcagac catcaaacat aggtatgctc
     1381 ggggcctggg tctcaggcgg tatagaatgg tgcgtgctgc atcatcacag ggcatcaacc
     1441 tttttgccga tggactatga aatggttgaa aatgcagacg gcagcaaaac aatctatata
     1501 ggtgagactg aaccccgtca ccgtattaga tggactattg gcatcacatc ctttccgggg
     1561 aaatcctact tcagggctga actgaagatc cacaacccga cacctttcaa tcattcattc
     1621 ctttactggg caaatgtggc cgcacatacg aacaaggacc tgcaggttat ttttcctccg
     1681 agtgttcagt ttgcaaccta tcacgccaag aatgatttca cacactggcc cttgtctacg
     1741 gaagtttatc agggacagga ttttacaaaa ggtgtggata taagctggtg gataaattcc
     1801 gttaatgcca attcatattt cgcacacgac ctgaaggagg atttcatggg tgcatacgat
     1861 catggcaggc agagcggcac ggttcacatc ggtgatcata acatagttaa gggagccaag
     1921 ttgtgggaat ggggatctgg tccgcgcgga caggcaacag aagcacggct gacagaaaat
     1981 gcaggaccct atgttgagat catggttggc gcttattctg acaatcagcc ggattacagc
     2041 tggataaaac cgtacgaggt gaaaatcagc gagcagtact ggtatccggt aaaggatatt
     2101 cagggtttca aaaatgcaaa tctcaatggt gctgtgaacc ttgaatcgag acagaaaaac
     2161 tccgtgtttc ttggttatta ctccacttca aaagttaaca aagccaggat cattttgaaa
     2221 aacagggata agataatttt cgaaaagctt acggagattt ctcctgtcac gacattcacc
     2281 cggaccgtga agatagacgg tcctttcagc cttacggatc tttataccga gatgactgat
     2341 acagaaactg gtgaagtact tgtttcttac cagccccggg aaaatgaata ccaggagaag
     2401 cttcccgaca ttgttgaagc tccgcctgct ccgaaggata ttgcaacagt tgaggaggtt
     2461 taccttaccg ggaaacgcct tgaacaattt cataactcga gatatgatcc tatggattac
     2521 tatgaggaag ccctcagccg tgatccggga gatatccgca cgaataccgc cgtaggaaat
     2581 atctgtctta aaaacggtga ttacataaat gccaggaaat atctggggag ggccataaaa
     2641 aggctaacaa aggattatac acgtccgatg gattgtgagg ccctgtatct tcagggctta
     2701 accttaaaag ccctcgagtt gtatgatgaa gctgttgaca ctctttacag ggcggcctgg
     2761 gattatccat tccatgcggc ttcctatctt gaacttgcaa gaatttcggt ttcaggcgga
     2821 gatctggaaa aagcactgca ccagataaac gaatcacttg cctccaatgc caggaataac
     2881 agttcaatca gtctgaaagc atccattctt aggaagaggg gcgattatat tgctgcagct
     2941 gccatcgtgg aaccgctgtt gcaaaacgat cctcttgatt tcagggctgc aaatgaatcc
     3001 tatctcctgg caaaggaatc aggcaacagg gaaaaagctg acagggaact tgcttcttta
     3061 aacaggaaga tgagaaattc tgatcagaat tatcttgaaa ctgcaatcgg gtacataaat
     3121 gacggattaa gggatgaggc agaagacctt cttctgcgtt tcaacggaaa aaatcccatt
     3181 atcagttatt acctggctta cctggcagat aaaagagggg ataaaacact tgcagagaaa
     3241 tatttcaaag aaggatcggc acagtcggag gactattgct tccctttccg ccttgaaacg
     3301 ataaaagtac ttgaaacagc actgaaatat aatcctgcag acggaaaagc acactattac
     3361 ctcggaaaca tattttatca cagaaatcag cgtgacaagg ccattgcaca atgggagacg
     3421 gctgtaggcc attcgcccgg actggctgtt gcccaccgta acctgggatg ggccttttac
     3481 aatcaccttg gtgacggatt taaggcaata agtgcatatg agaaagcaat tgaactgaga
     3541 agagacgagg ctatttacta tgaggagctg gatcatctgt atgaaatgag caatgccagc
     3601 atagcaagga gactgaaatt gtttgagggg agcaatgatg ttgtgtccgg acgggatgat
     3661 gcattcgcca ggcagattgc agtgctgaca ctgggggggc aggctgctga ggcagttgaa
     3721 tatcttagcg ggaaggaatt cagctatcgt gagggaagcg gaagggtgag agacatcatt
     3781 attgacgccc atctgatgct tggtctgaaa tatatggaag acaaggatta ccggaaagca
     3841 ctggataatt ttagtcttgc acaggtaccc gaggaagaag ccagcggcag cagggcaggc
     3901 aacaggaata ttcaggttta ctatttcctg ggtctggcca atgaggccct gggaaataaa
     3961 tcaaaagcca gaaactattt cgcctcgtgt gccgggctca gttcttcccc atctacctat
     4021 ataagctatt acagaggaat gagcaacctt aagctgggta agaaaaatga agcgctcaca
     4081 atcttcaatt caatgctcgc tgaagctgac aggcagctga gccagccgga agccccggat
     4141 gatttctttg caaaattcgg acagcgtgaa gcagagaatg ccagattgtc aaatatttat
     4201 ctgcttaaag gtctggcctg taaaggactt gctgatactg aaccggccgc tgaaaatctc
     4261 agcagggcag ttgacctgtc tgagagtaat ttatgggcca gatcggaatt aaaccgtctg
     4321 gattctactt attaatatcg tccatgtaag acaccagaca cttctggcgg ctatttatca
     4381 gtttgggcgg gtcatcggag atgaaaaaca gcctcatgca ccatgtaagg gcactaaggc
     4441 agataaaacc gaagatccat agctgcagac gtatcggtca gaatgtgatc attatcgggt
     4501 taaaaagata tgatttccgg acagttcagt acataccgta cacaagcgga tcagcatcct
     4561 tgtttacgat gataattcac gtttaatatg taaatttact aaaactgcta cattaattac
     4621 agaggcaacc cattcacaga tagggataaa tggccgtatc aggggatcat aaaggaaaag
     4681 agacggaact aatttttcac atacgttgat cttcacgagt atttgttcaa gattgagtat
     4741 gattctatta tagcaacata gattactttg acaataaaag ccgaatcaac ttcaggtgat
     4801 aaaaaacccg ttggctgtgg tgcagtagct atgggtcaga taatgtggta ttggcaatat
     4861 cctgcttctt acaattggga tataattcct actgcactcc acgaaggtga tgacgcggag
     4921 cctgcagcag aattagcaag actacttcgc gattgcggtt cggttgcaag aatgaattat
     4981 tgttgcaggg gttcttgggc aacgaccgaa aatgttgaac aaggacttaa ggatttagga
     5041 tttccaggag caacaaagag agttcggtca agctggccca gtagttcagg atggcaaaac
     5101 ttgattaggg ctgaattaga tgccggccgt cccgttcttt acaggggtgg tgctgttatt
     5161 gatcctggtg attggggcaa tgtccactac tttgtttgcg atggatatga cagaaacgac
     5221 ccttcctatt tccattttaa ttggggctgg ggtgacactc ccttaagcga tcctgactat
     5281 ggatattgtt ttactcttgc gaatttagct cctgatggta acaataatta taccagttat
     5341 cagcaaatga taataggaat ctctccaggg tgtgcaattc aggggagcat aaccagctta
     5401 ccttacacaa gcatttcaac tcctgtttct cttaatgcta acactatatc tataccgggt
     5461 gctggatcga ttttggaaat tgaacccgga ggaaacttat caatgtcggc aacaaatagc
     5521 attacattaa accccggctt ttttgcaaag gcaggaagtg tggttactgc aagtgtgggg
     5581 ccttatgagt gtgagtgtga tgatatatct gttcgtggct ggacaaattt ctttgcatgt
     5641 gataattgtg gtaaacaatt atacaattcc tatcaagtac tatcatacgg aagcccgtct
     5701 ctgatgttat catctccgga accagaagga gtgttaataa atgagacagg aatagaaact
     5761 aacaaggggg aaaatgaaaa caaggcagat gagcttcagc aggaagataa gaacatttta
     5821 ctggtcccca atccgaatga aggcaggttc cagttaagaa cagtatttga taactacagg
     5881 gttcaaatat ttaatccttc cggaaatctg atttatgata aatcaagccg tagaccaaca
     5941 atagacattg atttatcacg ttatccacaa ggaacatatt tcatgagatt gacgaccggt
     6001 ggaaatgttt ataccagtac aattttgaaa caatgaagcc ttttttaagt gtccttctat
     6061 tatttctgct ctgctcctgt ggtaaaaaag caacagacga actttgccct agatttttta
     6121 atagtacacc agggacgttg aatatcgtaa ctttttttaa gtcaaatgga acgaccagga
     6181 aagttgcccc caactctcta tcatcagtat ggatgattcc ttatactctg gatattctgg
     6241 atggtataaa tacacacctc gatagtattg ttgtttacga tgagaattct catttattat
     6301 gtaaattcag caagacatcc acatttaatt atcagggcaa tccgttcatt gatagggata
     6361 aatgggtata tcaaggaata gataggattc aaagactaag ccaatctatc acctacgtta
     6421 accttcacga atattggttc aaaattgaat atgagtctat tatagtccat ctatagatta
     6481 ctcggactgt aaaactgaat caacttcacg cgaaagcact tttttgttta ttccggcgta
     6541 attgacccct ctgctgaagg caacaactcc gaagggcgat cgtagttaaa tatgcaatga
     6601 acgattaatc aaaacaaaat tatacttttt tctttttctt cattgattct cccgagctat
     6661 ggaaagtttt ccataactcg ggcatgtcat atcgtgccaa agaaaaagag aaatggttaa
     6721 acagttaata taccttatag ttaaacccaa accctaaata aatagggaat agaaaaatga
     6781 aaaaaaccgc atgaatattt
//
LOCUS       DAOSPX010000169         6704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10540_length_6704_cov_4.070387, whole genome shotgun sequence.
ACCESSION   DAOSPX010000169 DAOSPX010000000
VERSION     DAOSPX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10540_length_6704_cov_4.070387"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLZ75_08885"
     CDS             <1..284
                     /locus_tag="PLZ75_08885"
                     /inference="COORDINATES: protein motif:HMM:NF036579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS72545.1"
                     /translation="GPVDEITHCSYENLTPYGMASISWEKQNNKFFTEINVPVGSSAI
                     IYLPCGDKGPVRENGRRTGKRSGIIFREFTGRHAVYEVGSGKYRFESTM"
     gene            308..1792
                     /locus_tag="PLZ75_08890"
     CDS             308..1792
                     /locus_tag="PLZ75_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS72546.1"
                     /translation="MNNLQLLTGCICGISVLTSVVSCRRDDGKPNVLVIFTDDQRADA
                     LGCSGNRYIRTPNIDNLAGNGVRFLNCYVMGGHHGAISAPSRAMLLSGKYLFNVYDRL
                     DGVKTMPMHFAEHGYVTFGTGKWHNEKSAFEASFQKGRNIYLGGMADHFNIPVCDLGD
                     DGKLSEPVYKGFSTDIIGDAAMEFIRDYADGDRKNPFFCYVAFTAPHDPFSPREDYID
                     MYSPASVELPGNFLPLHPFAFDDLMVRDELLTDWPRDPQIIKSILADYYGLISHLDAK
                     VGEIIRTLKEKGLYENTIIVYAADNGLALGSHGLIGKQNLYEHSMNVPFIIAGPGVPE
                     GKISDALVYLLDIYPTLTGLAHIPFPEGTDGENLVPVITRESKEVRSSLFTAYRNTVR
                     AVRTKEWKLIRYPELDKTQLFNLANDPLETDNLAEDETFAGKTEALLEIMKKWQQSTG
                     DTVPLTAREIKPSDYDYRKLVRKPDRWQPEYTLKKYFGQDGQED"
     gene            1980..2924
                     /locus_tag="PLZ75_08895"
     CDS             1980..2924
                     /locus_tag="PLZ75_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOS72547.1"
                     /translation="MKIVVLDGYALNPGDLSWEGLKSLGEVEIYDRTSAEETVRRASG
                     AEIILTNKTMLLSDVIGKLPDLKYIGVLATGYNVVDLDITRERGIVVTNVPAYSTMSV
                     AQHVFALILEFFNVPCSLSEGVRNGRWSRNIDFCYWDKTLTELDGLVMGIVGFGKIGQ
                     AVARIGEVFGLNVLVHTRSRVSDYENCSLEQLLEKSDIVSLHCPLTPETRGMINAERL
                     GMMKPSALLVNTARGPLVIEQDLADALNKGIIAGACLDVLSQEPPGEDNPLLSARNCI
                     ITPHVAWATLAARKRLMKIAVSNVREFLAGSPVNVVNM"
     gene            complement(2967..5531)
                     /locus_tag="PLZ75_08900"
     CDS             complement(2967..5531)
                     /locus_tag="PLZ75_08900"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HOS72548.1"
                     /translation="MKTNLNQVLSAFFFLFIISGCGTHEKAGSDIRKVGPQPDGSILV
                     PSNQFLRPAGFQVEIPGRPVDLILTADERFLLVKNKNDLDLIRLIDRTVLQSLPYEKS
                     GASFTGICLSPDGRTVYLTDAQNNICVASLDKNNILKWEDPVHLPGPSFGGYPAPGGV
                     AVNDKGDKLYVTLSRNNSLGIVTLPSKSVSEIPVGIAPYDVIIHSPSKLYVSNWGGRQ
                     PEEGEPVYKTSGSDVLIDPETGIANNGAISVVDPEKKIQLKVIEVGLHPSGMALSPDK
                     KLLYVACANSDQISVISTDNDEVIETISVHMLDNIPFGSGPGDLTVSPDGKYLYVANG
                     TENSICVIKTGREAENETEAEIAGSIPVGWYPGSVVLDKAGKTLFVANVKGTGSRNQR
                     TDAKGYSSHDHMGSVSIIKVPDRRELRKMTETVNLNNSYAEMLKKLNPSKKTKKKVPV
                     PEFPDQTSFFRHVVYIIKENRTYDQVFGDMPMGDGDTSLVQFGREITPNHHLLAETFV
                     LLDNFYCSGVLSADGHQWTDEAYVTDYLEKSFGGFTRSYPYDGGDPLAYASSGFIWDN
                     VLRHGLTFRNYGEFVEAVIEPASATFTDIYRDFLEGSNKITIRAKAKLEQLTPYTCPT
                     YIGFPNKVPDVYRAAEFIKELKEFEKNDNFPNFIIMLLPNDHTSGTRPGMPTPQSAVA
                     DNDLALGQIVEAISTSKFWKETCILVTEDDPQAGLDHVDGHRTVGLVISPYTRRGEVI
                     STYYSQINMVRTIENILGLPPMNQLDMTAEAMTDCFTGKPDFTPYKAVKNNIPLDNIN
                     PPLTSLSGDQLYWAKKSMEQDLDDVDRIDEDTFNRIIWHAVKGYNRPYPVITDRNF"
     gene            complement(5635..6636)
                     /locus_tag="PLZ75_08905"
     CDS             complement(5635..6636)
                     /locus_tag="PLZ75_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HOS72549.1"
                     /translation="MFIPQTYSLAVILCIITMLCWGSWGNTQKLAGKTWRFELFYWDF
                     VTGILIFSLLFGFTLGSIGEHGRSFVPDIIQADAGNILNAFLGGVVFNASNILLVAAM
                     AIAGMAVAFPVGVGIALALGVIINYIFTPKGDPVILFSGLALIVAAIVIDAIAYKKHS
                     TTLQKVSGKGILLSVGAGVLMSLFYRFVASSMVTDFTNPEAGRLTPYSALFFFAAGVL
                     VSNFLFNYILMKKPVEGSPLTFSDYRKGSAKVHLIGILGGLIWNLGMSMSILAADKAG
                     FAISYGLGQGATLVAAFWGVFIWKEFKGAGKTVNTLLFLMFMAYLAGLALLVYAGTR"
BASE COUNT         1847 a         1483 c         1559 g         1815 t
ORIGIN      
        1 ccggaccggt agatgaaatc acacattgtt cctatgagaa tctgactccg tacggaatgg
       61 cttcaatcag ctgggaaaag cagaataata aattcttcac ggaaatcaat gttccggtgg
      121 gttcatctgc aattatttac ctgccatgtg gtgataaagg tccggtaaga gagaacggga
      181 gacggaccgg gaagagatca ggcataatat tcagggaatt caccggcaga catgctgttt
      241 atgaagtggg ctccggaaaa tacagatttg aaagcactat gtaaattaat aaattaaaca
      301 aattcctatg aataatcttc agctgcttac cggatgtatt tgcggcatat cggtactgac
      361 atccgttgtt tcgtgccgca gggatgatgg caaaccaaat gtgcttgtaa tttttaccga
      421 cgaccagagg gcagatgcgc tggggtgttc ggggaacagg tacataagaa cccctaatat
      481 tgacaatctt gccgggaatg gtgtccgctt cctcaactgc tacgtcatgg gagggcatca
      541 cggcgccatt tcggctccga gccgggcaat gctgctcagc gggaaatacc tgttcaacgt
      601 gtatgacagg cttgacggcg tgaaaacaat gccaatgcat tttgcggaac atggttatgt
      661 tacattcgga acaggcaaat ggcataatga gaaaagtgct tttgaagctt catttcaaaa
      721 aggcagaaat atttaccttg gaggaatggc tgatcatttt aatattcccg tatgtgatct
      781 gggtgacgac ggaaaattat ccgagccggt ctataaaggt ttttccaccg atattatcgg
      841 cgatgcggca atggaattta tcagggatta tgcggacggg gacaggaaaa acccgttttt
      901 ctgctatgtt gccttcactg caccgcacga tcctttttcg ccgcgggagg attatattga
      961 catgtattct cctgcttccg tggaactgcc gggaaatttt ctccccctgc atcccttcgc
     1021 tttcgatgat ctcatggtaa gggatgagct tcttacggac tggccccgtg atcctcagat
     1081 aatcaaatca atactggctg attattacgg actgatatca catctggatg caaaagtcgg
     1141 tgaaataatc cgcacgctga aggaaaaagg attgtatgaa aatacaataa ttgtgtatgc
     1201 agccgataat ggcctggccc tgggaagcca cggacttata ggaaaacaga atctttatga
     1261 gcacagcatg aatgtaccct ttatcattgc cggaccggga gttccggaag gaaaaatatc
     1321 cgatgccctt gtttaccttc tcgatatcta tcctaccctg accggactgg cacatatacc
     1381 gtttccggaa ggaacagatg gcgaaaacct ggttccggta atcacaaggg aatcgaagga
     1441 ggtacggtca tcccttttca cggcataccg caacacggtc agggctgtaa gaacaaagga
     1501 atggaaactg atacgctatc ctgagctcga taagacacaa ctgttcaatc ttgcgaatga
     1561 tccgctggag acggataatc ttgcggaaga tgagaccttt gccggaaaga cagaggctct
     1621 gctcgagatt atgaagaaat ggcagcaaag cacaggtgat acagttccac tgactgcccg
     1681 ggaaataaaa ccgtctgatt acgattacag gaagcttgta aggaaaccag acaggtggca
     1741 gccggaatat accctgaaaa agtatttcgg acaggacggg caggaagact aaacggcttc
     1801 ttctgtgatt caggcaaaac caggtctggc agatttcccg ggcaggaaaa agagctgttt
     1861 ctgcttcgaa aatcccggtt tttttctatt attgtacagg aaatgtgtat ttgttgcacg
     1921 ggaggaacaa atacactttt tttaagtatt tgtggataat ccggaattaa aaaaaccata
     1981 tgaaaattgt agtacttgac ggatacgcac ttaatcccgg tgatctttca tgggaagggc
     2041 tgaaatctct gggggaagtg gaaatatatg accgtacatc tgctgaagaa acagtcaggc
     2101 gtgcttcagg agctgagatt atactgacaa acaaaaccat gctgttgtct gatgtcatcg
     2161 ggaaactgcc ggatctgaaa tacattggtg tgctggctac aggatacaat gttgttgacc
     2221 tggacattac acgggaaagg ggtattgttg taacgaatgt tcctgcctat agtacaatgt
     2281 ccgtggccca gcatgttttt gcgctgatac tcgaattctt taacgtcccc tgttcattgt
     2341 cagaaggtgt caggaacggc agatggagcc gtaatattga tttttgctac tgggacaaga
     2401 ccctgactga acttgatggc ctcgttatgg gtattgtcgg ttttggaaag ataggacagg
     2461 cagttgcacg tatcggggag gtattcggat tgaatgtgct ggttcatacc agaagcaggg
     2521 taagtgacta cgaaaactgc tccctggaac aacttttaga gaaaagtgat atagtctccc
     2581 ttcattgccc gctcactcct gaaacacggg gaatgatcaa tgctgaacgc cttggcatga
     2641 tgaagccgtc agcacttctc gtcaatactg cacgcggccc gcttgttatt gagcaggatc
     2701 ttgctgatgc actcaataag ggaataattg caggtgcgtg cctcgatgtc ctttcacagg
     2761 aaccgccggg ggaggataac ccccttctct cagccaggaa ttgtattata accccgcatg
     2821 ttgcgtgggc aaccctggcc gcaaggaaaa ggcttatgaa gattgcggtc agcaatgtca
     2881 gggaattcct ggcaggcagt cccgtgaatg tggttaacat gtaaaacttc cggaaatagc
     2941 cgggtcactt tttgtgacgt cttttgtcag aagtttctgt cagtaataac cggataaggc
     3001 ctgttatatc cttttacagc atgccatatt atacggttga acgtatcttc gtcaatacga
     3061 tcaacatcat caagatcctg ctccattgat ttcttcgccc agtaaagctg atctcccgag
     3121 agggatgtca acgggggatt gatattatca agggggatat tgtttttcac tgctttgtac
     3181 ggggtgaaat caggtttgcc ggtaaaacag tccgtcatgg cttcagcagt catatccagc
     3241 tggttcatag gaggaagccc gagaatgttt tctattgtcc gaaccatatt aatctgggaa
     3301 taataggttg atatcacttc tcctcttctg gtataaggac ttatcaccag accaacggta
     3361 cgatggccat caacatggtc gaggccggcc tgaggatcat cttctgtaac cagaatacag
     3421 gtttctttcc agaatttact ggttgatatt gcttcaacga tctgtccaag tgcaagatca
     3481 ttgtcagcaa ccgcagactg tggtgttggc ataccgggac gtgttcctga agtatggtca
     3541 ttaggaagca gcattattat gaaattcgga aaattatcat tcttttcgaa ctctttcaat
     3601 tccttaatga actcagcggc acggtaaaca tccggtactt tgtttggaaa accgatataa
     3661 gtcggacagg tgtacggagt taactgttcc agttttgctt ttgcacggat tgttatttta
     3721 ttgcttcctt cgaggaaatc acgataaata tctgtaaagg tagccgatgc aggttctatc
     3781 accgcttcta caaattcccc gtaattcctg aatgtcagtc cgtgtctgag tacattatcc
     3841 catataaagc ccgaactggc ataagccagg ggatcaccgc catcataagg ataactgcgg
     3901 gtgaatcccc caaatgattt ttccaggtaa tctgtcacat atgcctcatc tgtccattga
     3961 tgtccgtctg cacttaaaac accactgcaa taaaaattat ccagcaggac aaaagtttcg
     4021 gcaagcaggt gatggttagg agtaatttcc cttccgaatt gtaccaggct tgtatcaccg
     4081 tcacccatgg gcatatcccc gaaaacctgg tcgtaagtgc ggttttcctt aattatataa
     4141 acaacatgcc tgaagaaaga agtttgatcc gggaattcgg gaacaggcac ttttttcttt
     4201 gtttttttcg aggggttaag ttttttcagc atttcagcat atgagttgtt cagattgact
     4261 gtttcagtca ttttcctgag ttcccgcctg tccggaactt ttattatcga cacggaaccc
     4321 atgtgatcat gtgaactata acctttagca tcagttctct gatttctcga tccggtgcct
     4381 ttgacatttg ccacaaacag agttttgccc gccttatcca gcacaacaga acccggatac
     4441 cagcctaccg gtattgaacc cgctatctca gcctcagtct cattctcagc ctcccggccg
     4501 gtttttatga cacagattga attttccgta ccattagcca cgtacagata tttgccgtca
     4561 ggtgatacgg tcaggtctcc cggtccgctc ccgaagggga tattatcaag catgtgcact
     4621 gaaatcgttt caatgacctc gtcattatcg gtactgatga ccgatatctg atcgctgttt
     4681 gcacaggcaa cataaaggag ctttttatcg ggactgagag ccatacctga aggatggaga
     4741 cccacttcaa taactttcag ctgaatcttc ttttccggat cgaccactga tatagctcca
     4801 ttgttagcaa ttcctgtttc cggatcaatc aggacatcac tgccggatgt tttgtaaacc
     4861 ggttctcctt cttccggctg acggcctccc cagttgctga catataattt tgacggggaa
     4921 tggataataa catcataagg ggctattccg acaggaattt ccgaaaccga ctttgaagga
     4981 agagttacta tccccaaaga attgttacgg ctcagggtta catatagttt atcaccttta
     5041 tcatttactg caactccacc cggtgcagga tatccgccaa atgaaggccc tggtaaatga
     5101 accggatcct cccatttcag aatgttgttt ttatcaagtg atgccacaca tatattgttc
     5161 tgtgcatctg taagataaac cgtacgtccg tccggcgaaa gacaaatgcc ggtaaaagaa
     5221 gctccgctct tttcataagg cagtgattgt aaaacagtcc tgtcaataag tctgatcaga
     5281 tcaagatcat tcttgttttt caccagaagg aatctttcgt cggcagtaag aatcagatcc
     5341 acaggtcttc cgggaatttc tacctgaaac cctgccggcc ttaaaaactg gtttgaagga
     5401 acaaggatgc tgccgtcagg ctggggtccc actttcctta tatcactgcc tgctttttca
     5461 tgtgttccac atccggatat tatgaacagg aaaaagaacg cagataaaac ctgattcaaa
     5521 tttgttttca ttttttaaag catttttaaa ttaaattcta tatattgggg tttcagagtt
     5581 ccgggttaaa ggttggggga tcacattaaa aatgtcccgc tgccgttttt cgttttagcg
     5641 ggtcccggca tagacaagca gggccagtcc tgcaagatat gccataaaca taaggaacag
     5701 aagggtgtta actgttttac cggctccttt gaattccttc cagatgaata caccccagaa
     5761 tgccgcaaca agtgttgctc cctgccccag gccgtaagag atggcaaaac cggccttgtc
     5821 agcagccaga atactcattg acatacccag attccagatc agtcctccca ggattcctat
     5881 aagatgaacc tttgcgcttc ccttcctgta atcactgaat gtaagagggc tgccctccac
     5941 cggttttttc atcaggatgt agttgaagag aaaattgctt acaaggacac cggcggcaaa
     6001 aaagaaaagt gccgaatagg gagtaagtct gccggcttcg ggatttgtaa aatcagtaac
     6061 catggaggaa gccacaaaac ggtagaaaag tgacatgaga acgcctgcgc cgacagagag
     6121 gaggatccct tttcctgata ccttctgcag ggttgtgctg tgttttttat aggctatggc
     6181 atcgatgact attgcagcaa cgataagtgc cagaccgctg aaaagtataa ccggatcacc
     6241 tttcggagta aaaatataat tgatgatgac cccaagggca agggctattc ctaccccgac
     6301 gggaaaagca actgccattc cggcgatcgc cattgctgcc accaggagta tgtttgatgc
     6361 attgaagacc actcctccca ggaaggcatt taatatattc cccgcgtccg cctggataat
     6421 gtcaggaaca aaacttcgtc cgtgctctcc gatactgccg agggtaaagc caaaaagcag
     6481 tgaaaagatc agtatacccg ttacaaaatc ccagtaaaac agttcaaatc gccatgtttt
     6541 accggcaagt ttctgggtat ttccccagga accccagcat agcattgtta taatgcagag
     6601 tattactgca agtgaatagg tttgtggaat gaacataatg actgatttat taattagtgt
     6661 ctgtctttaa actcaaattg ttaataaata cagggagtta ccaa
//
LOCUS       DAOSPX010000170         6696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10548_length_6696_cov_4.314561, whole genome shotgun sequence.
ACCESSION   DAOSPX010000170 DAOSPX010000000
VERSION     DAOSPX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10548_length_6696_cov_4.314561"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLZ75_08910"
     CDS             complement(<1..257)
                     /locus_tag="PLZ75_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72550.1"
                     /translation="MEKKYVLLQNELASVYSREGKELSPDSISGAVFDEILTTVSSYL
                     ALFRRKEDGRFYITGLNSKVEETELIDKDEVAGKPVESTPLS"
     gene            complement(354..1019)
                     /gene="nth"
                     /locus_tag="PLZ75_08915"
     CDS             complement(354..1019)
                     /gene="nth"
                     /locus_tag="PLZ75_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOS72551.1"
                     /translation="MTKKERYRLTFEYFKKNCPVVETELNYRDPFELIVAVILSAQCT
                     DKRVNLITPALLKRFPDAKSMAAGEQSEIFDFIRSCSYPNNKARHLSEMARMLVDEFD
                     CKVPSEPELLQKLPGVGRKTANVVASVVYKKPVIAVDTHVFRVSRRIGLTTNAKTPAE
                     AEKQLTRNIAPELRHDAHHWLILHGRYVCKARKPLCEECGLNSFCKYYHSVVLKGTGE
                     ARI"
     gene            1124..2158
                     /gene="aroB"
                     /locus_tag="PLZ75_08920"
     CDS             1124..2158
                     /gene="aroB"
                     /locus_tag="PLZ75_08920"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOS72552.1"
                     /translation="MEEIIVNAGGCLSRIFVGQHREDIARMIPGKGVVIITDDNVYSV
                     YGKEFPGFPVLRIKPGEGSKQLKTVELLAAGLLEHNIDRSGFLLGIGGGVVCDITGFL
                     ASVFMRGISFGFVSTSLLSQVDASTGGKNGVNLGEIKNLIGIFRQPEFVVCDTEMLKT
                     LPEDEYFSGLAELIKTGIIGDPSIIESLEKDHQKVRNRDPELLSDLVGRAVKYKASVV
                     TADERESGLRRVLNFGHTFGHAIELHKGIKHGFAVAAGMELSLRWSRELEYIDNDTCE
                     RILNLFSLYNLRFDWHMSPDDMRKLISHDKKKSGGDIYFVFVKGTGKPFVKLLPVSEL
                     IDFYSRITRK"
     gene            2167..3171
                     /locus_tag="PLZ75_08925"
     CDS             2167..3171
                     /locus_tag="PLZ75_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOS72553.1"
                     /translation="MNSFGTLFRISIFGESHGKAIGVAVDGCPPGIALEPEDFLPDLR
                     RRKSGASGTTKRHEPDLPEVLSGLYGGFTTGSPMTIMTWNQDKISSDYDEFFRIPRPG
                     HADFTSTVKYSGFSDRRGSGHFSGRITWGLVVAGVVAKRIAATAGFSAKLISAGGSSD
                     IEGAVRKAVQENDSIGGIIECVVSNVPAGLGEPFFNSVESVISHLVFSVPAVRGIEFG
                     SGFSAASMRGSEHNDPFTDDRGSTSTNNAGGINGGITNGNDIVFRVAIKPASSIGVAQ
                     TTFNFNSKKMDTLEVKGRHDTCIALRIPVVIEAAAAIAVADLMQADRGIYNAERKTRS
                     "
     gene            complement(3230..4555)
                     /gene="tilS"
                     /locus_tag="PLZ75_08930"
     CDS             complement(3230..4555)
                     /gene="tilS"
                     /locus_tag="PLZ75_08930"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOS72554.1"
                     /translation="MLKEFKEYIEQNGLIRENEKILLAVSGGIDSMVMTDLFIRAGQE
                     IGIAHCNFTLRGEESQADKEFVAKYAADHNIGFFSVRFDTRAYASEKGISIQMAARDL
                     RYSWFEKTREKQGFDLIAIAHNLNDNIETFLINLVRGTGITGLSGIRKTRDRVIRPLL
                     FAGRKSIEDYSKTNGIPYREDSSNTDTGYVRNRIRHLVLPLLAEINPSVENALNDTSK
                     RLSEINEVFTAFTADLRKKLIRKDDSLLIIDLEKLEPFISSNAVMFELFRPFGITSAL
                     LCDLLNVISGQTGSGIRTLSHRIIKNRNELIISETTPKQSITRQFNTIGELSACPFIS
                     SVREQNVSGSYSIPSSGNTAWFDREKITFPLTIRNWQHGDYFFPLGMQHRKKLSDFLT
                     DIKESLFTKENTLVLVSGGEIAWVMGKRIDDRFKVTGKTRKVLEITVKS"
     gene            complement(4568..6505)
                     /locus_tag="PLZ75_08935"
     CDS             complement(4568..6505)
                     /locus_tag="PLZ75_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72555.1"
                     /translation="MEIDLKYTCKAGPLIILTLLFFHSGLHSQKPPENDTIFQSIPGN
                     HIKTPDPRLFSDAGWPVTVPQKLIPPTFRDNDRSFFTDTLRAKASKYLITKKLFDFVV
                     VSGKPASAASAEGSSQNDYLRYSGRIIRKIDIKRLDVFGTSIDYSLISNSGRLENFLN
                     KTHTNTAEHIIRKNLLFSEGDTLSPLLLSDNERILRQLPYIDDSRIIVVSETDGFVDI
                     LVLTKDVYSLGASAEFRSATRGSASLYEKNIFGLGHEFRLKVPFDQDSAGSPGIGMNY
                     NINNISKSFINLSLRYYNALGRTSYGFAADRQLVSSSTKYAGGICVCQTSTTTDLDTL
                     PEPEPLKFNHQDYWLSRSFLLKPENAARLILGVRYLNNNVYRHPDILPDSYHSLQKYR
                     IYLASLSFSIRQFYKTSLIYGHGRTEDLPDGMLINLTAGRELNEFKQRFYSGLSLSAG
                     KKLSGGGYFHGNAGLASFRNKNETEQGILILRANYFTKLRYAGTHRLRNFYNIDYTRG
                     FGRYKDEYLLFSTENGLTGFRNDSIRAERQRLTMSIESVLFSPARIYGFRLAWFGFGD
                     LGYLFGTNQFFNQGKVISRIGFGVRLRNDNLVFNTLQIRFSFFPNLPPYSRISNFNVS
                     GEQLLNPDNFEPGRPDLLPYR"
     gene            complement(6507..>6696)
                     /locus_tag="PLZ75_08940"
     CDS             complement(6507..>6696)
                     /locus_tag="PLZ75_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72556.1"
                     /translation="VIATAVQYSLEFDLLVPPYDTVKIATVGQLEAKIQHSRVITGKR
                     LGFQFNSDDVLITEAHSK"
BASE COUNT         1698 a         1444 c         1645 g         1909 t
ORIGIN      
        1 gaaagcggtg ttgattccac aggcttgccg gcaacttcat ccttatcaat cagttctgtt
       61 tcttcaacct tgctgttaag tccagtgata tagaagcggc catcttcctt ccttctgaaa
      121 agggcaagat aactgcttac cgttgtcagt atttcatcga aaacagctcc acttatgctg
      181 tcgggagaca attccttccc ttcacgtgaa taaacagatg caagttcatt ctggagcaga
      241 acatacttct tctccaattc cctaaaccta gttttgtaat tcatcagaca gttagtattg
      301 cgccctaaaa ttagcaaata aagtgctaac tgcaatagct ttagcgtgaa aaatcagatt
      361 ctggcctctc cggtgccttt caggactaca gaatggtaat atttacaaaa ggaattcaga
      421 ccacattcct cacacagggg ctttcttgcc ttgcatacgt accggccgtg aagaatcagc
      481 cagtgatggg catcatgcct gagttccggg gctatatttc ttgtgagctg tttttcggct
      541 tctgccggtg tttttgcatt cgtggtaagt cctattctcc gggataccct gaatacatgc
      601 gtgtcaacag caataaccgg ttttttataa acgacggagg ctactacatt ggcagtcttt
      661 cttcccactc ccggaagctt ttgcaataac tccggctcgg acggaacctt gcagtcaaat
      721 tcgtcaacaa gcatccttgc catctctgag agatgccttg ccttgttgtt gggatatgaa
      781 cacgacctga taaagtcaaa aatttccgat tgttcgcctg ccgccattga ttttgcatcg
      841 ggaaatcttt tcagcagggc gggtgtgatc agattgaccc ttttgtcggt acactgggcg
      901 gaaaggatta ccgccacgat cagttcaaag ggatcccggt agttcagttc ggtttccaca
      961 accggacagt tctttttgaa atattcaaag gtaagcctgt atctctcctt tttcgtcata
     1021 gataatacgc ctcggaaagg aggtgaagtt aatgaatttt atatttgcaa agcggactca
     1081 taaaatcagg aatttttaac tttatcgcaa aattaaaact gctttggaag agataattgt
     1141 aaacgcggga ggatgcctgt cccgtatatt tgtcgggcaa caccgggaag atatcgccag
     1201 gatgatcccc gggaaggggg ttgttatcat taccgatgac aatgtatata gtgtttacgg
     1261 aaaggaattc cccggtttcc cggtattgag aatcaaaccg ggagagggaa gcaaacagct
     1321 gaaaactgtt gaattacttg cagccggatt gctggaacat aatattgacc gttcgggatt
     1381 cttactgggc ataggaggcg gagttgtctg tgatattacc ggctttctgg cttcggtctt
     1441 catgcgcggc atttccttcg gctttgtttc caccagcctg ttgtcgcagg tggatgccag
     1501 caccggggga aagaacggtg tcaatctggg agagataaaa aatcttatcg gtatcttcag
     1561 gcagccggaa tttgttgtct gtgatactga aatgctgaag acacttccgg aagatgaata
     1621 tttttccggg ctggcagaac ttatcaaaac gggaatcata ggggatcctt ccattattga
     1681 atcactggaa aaggatcatc agaaggtcag gaatcgtgac ccggagctcc tttcagacct
     1741 ggttggcagg gcggtaaaat acaaggcttc ggtcgttacg gctgatgaaa gggaatcggg
     1801 cctgagacgt gtgctcaatt tcggacacac attcggccat gccatagagc tgcataaggg
     1861 gataaagcat ggttttgccg ttgcggcagg catggaatta tcccttcgct ggtcaaggga
     1921 actggaatat attgataatg acacctgtga acggatttta aatctcttca gcctgtataa
     1981 tctccggttt gactggcaca tgtcgccgga tgacatgagg aaactgataa gtcatgataa
     2041 aaaaaagtca ggcggtgata tttattttgt ttttgtaaaa ggaacaggga aaccctttgt
     2101 aaaactgttg cctgtaagtg aattaattga tttttacagt cggataacca ggaaataaca
     2161 gaaaatatga attcattcgg aactttgttc aggatatcga tattcgggga atcgcacggg
     2221 aaagccattg gtgtggctgt tgacggatgt ccgccgggaa tagcccttga accggaagat
     2281 tttcttcccg atctgagaag gcgcaagagc ggtgcttcgg gtaccaccaa acgccatgaa
     2341 cccgatttgc ctgaggttct cagcggtctg tacggcggtt ttacaacggg atcacctatg
     2401 acaatcatga cctggaatca ggataagata tcttccgatt atgatgagtt tttcagaatt
     2461 ccccggcccg ggcatgcgga ttttacgtct acggtaaaat actccggttt ttccgaccgc
     2521 agggggagcg gccatttttc cgggcgtatc acctggggac tggtggttgc cggggttgtt
     2581 gcaaaaagga ttgccgccac ggccggattt tccgcaaaac tgatttctgc aggcggatct
     2641 tcagatattg agggagccgt aaggaaggca gtgcaggaaa atgattccat cggggggata
     2701 atcgagtgtg ttgtaagtaa cgttccggcc ggcctggggg aacccttttt caattcagtg
     2761 gaatcagtga taagccatct tgttttttcg gttcctgcag taaggggtat agaattcggt
     2821 tccggatttt cggcagcctc aatgagggga tcggaacata atgatccttt cacggatgac
     2881 agaggcagta cctctacaaa taatgccgga ggcataaacg ggggcattac gaatggaaat
     2941 gatatagttt tcagggttgc gataaaaccg gcatcctcga tcggagtggc ccagaccacc
     3001 tttaatttca attcaaaaaa gatggatacc ctggaggtga agggccggca tgatacctgt
     3061 attgcactga ggatacccgt ggtcattgaa gctgccgctg caattgcggt ggcagacctg
     3121 atgcaggcag acagggggat atataatgct gaaagaaaga caaggtcata ggatctcctt
     3181 cctgcatcat ttgaagatca cattgttaaa gggatattaa actgttcagt caggatttca
     3241 ctgtaatttc cagcactttc ctggttttgc cggtaacctt gaacctgtcg tcaattcttt
     3301 tccccatgac ccaggcaatt tctccccccg ataccagtac cagggtgttt tcctttgtga
     3361 aaagagattc ctttatgtcg gtcaaaaaat cactgagctt tttcctgtgc tgcattccga
     3421 ggggaaagaa ataatctccg tgctgccagt tccttattgt aagagggaaa gtgatttttt
     3481 ccctgtcgaa ccaggcggta ttgccggagg agggaatact gtagctcccg ctgacatttt
     3541 gttctctgac agaactgatg aaagggcatg cggaaagttc cccgatggta ttgaactgcc
     3601 gggttatact ctgtttcgga gttgtttcac ttatgataag ctcgtttctg tttttaatga
     3661 ttctgtgact gagggttctg atcccgcttc cggtttgtcc cgaaattaca ttcagaagat
     3721 cgcacaggag ggcagaggtg attccgaacg gcctgaaaag ctcaaacatt acagcattac
     3781 tggagataaa gggctcaagt ttttccagat caattatcag aagcgaatca tctttcctta
     3841 tcagtttttt tcgcagatct gcggtaaagg cagtaaaaac ctcattgatt tcgctgagtc
     3901 tttttgaggt gtcgttgagt gcattttcaa cggaaggatt tatttccgca agcaggggca
     3961 atacaagatg gcgtatcctg ttccgtacat atcccgtgtc ggtattggaa ctgtcctccc
     4021 ggtaggggat gccgttggtt ttggagtaat cctcgattga cttcctgcct gcgaacagaa
     4081 ggggccggat caccctgtcc cgggtttttc tgatgcccga caggccggtg attcccgtac
     4141 cgcgcacaag gttgatgagg aatgtttcaa tattgtcgtt aagattgtga gcaatggcaa
     4201 tcaggtcaaa tccctgtttt tctctcgttt tttcaaacca tgaatatctc aggtcccttg
     4261 cagccatctg gatggatatt cctttctccg aggcataggc cctggtatcg aatctgacag
     4321 aaaaaaaccc tatgttatga tctgccgcgt attttgccac aaattcttta tcggcctgtg
     4381 attcttcccc cctcagggta aaattgcagt gggcaattcc tatctcttgt ccggccctga
     4441 tgaaaagatc ggtcattacc attgaatcaa tgccaccgct gacagccagc agaattttct
     4501 cattctctct tatgagtccg ttttgttcaa tatattcctt aaactctttc agcattgact
     4561 catattttta tctgtatgga agcagatccg gcctgcccgg ctcaaaatta tcgggattga
     4621 gcagctgttc cccggaaaca ttgaaattac tgattcttga atagggtggg agattgggaa
     4681 aaaagctgaa tctgatctgc agggtgttga atacgagatt atcatttctc agtctcactc
     4741 caaaccctat tctggaaata acttttccct ggttgaaaaa ctgatttgtt ccgaaaagat
     4801 atccgagatc accgaagcca aaccatgcta agcggaagcc gtatatccgg gccgggctga
     4861 aaaggactga ttcaatactc attgtcagcc gttgtctttc tgccctgatt gagtcattcc
     4921 tgaatccggt cagaccgttt tcggtactga aaaggagata ctcatcctta tatcttccga
     4981 atcccctggt atagtcgata ttgtaaaaat tcctcagtct gtgggtgccg gcgtaacgga
     5041 gttttgtaaa ataatttgcc ctgagaatca ggatgccctg ttcagtttca tttttatttc
     5101 tgaaggaagc caggcctgca ttgccgtgaa aatatcctcc gccgctgagt ttctttcccg
     5161 cggaaaggct gaggcctgaa taaaaacgct gtttgaattc atttaattca cggccggcag
     5221 taaggtttat cagcattccg tcaggaaggt cttctgtcct cccatggcca taaatcaggc
     5281 tcgttttata aaactgccgg attgaaaagg acagtgatgc aaggtagata cggtatttct
     5341 gaagggaatg ataggaatcc gggagaatat ccggatgcct gtacacattg ttgttcagat
     5401 atctgacacc gagtatcagt cttgcagcat tttccggctt cagcaggaac gacctggaaa
     5461 gccagtagtc ctgatgattg aatttaaggg gttccggttc cgggagtgta tccaggtctg
     5521 ttgtggttga agtctggcag acacatatac cccctgcata ttttgttgag gaactgacaa
     5581 gctgcctgtc ggctgcgaag ccgtagcttg ttctgccaag tgcgttataa taccggaggc
     5641 tgaggttgat gaaggatttt gaaatattgt tgatattata gttcatgccg attccgggcg
     5701 atccggccga atcctggtca aatggtacct tcagtctgaa ttcatggccg agtccgaata
     5761 tattcttctc atacaggctt gcacttcccc tggtggcaga cctgaattct gctgaagccc
     5821 caagtgaata cacgtctttt gtaagtacga ggatatccac aaagccgtca gtttcggata
     5881 cgacaattat ccttgaatca tcaatatagg gcagctgcct gagtatcctc tcgttatcac
     5941 tgagcagaag aggtgaaaga gtatcaccct cgctgaaaag aagatttttc ctgattatat
     6001 gttccgccgt gtttgtatgg gttttgttga ggaagttctc aagcctgccg gagttgctta
     6061 tcaatgaata atctatgctg gtaccgaata catcaagtct tttaatgtct attttcctta
     6121 ttattctgcc ggaatagcgg agatagtcgt tctggcttga gccttcggct gaagcggcag
     6181 aagcgggttt acctgaaacg accacaaaat caaagagttt ttttgttatc aggtattttg
     6241 aagctttcgc cctcagggtg tcggtaaaaa aacttctgtc gttgtcccgg aatgtgggag
     6301 ggataagttt ttgcgggact gttaccggcc agcctgcatc ggaaaaaagc cggggatccg
     6361 gtgttttaat atggttgccc ggtatgcttt gaaatatagt gtcattttca ggaggctttt
     6421 gtgaatggag gccggaatga aaaaacagca gggtcagaat gatcaggggg cctgccttac
     6481 acgtatattt caggtcgatt tccacatcac tttgaatgag cctcggtaat gagcacgtcg
     6541 tcagagttga actgaaatcc cagccgtttt cccgtaatca ccctggaatg ctgaattttg
     6601 gcttcaagct gtcctacagt ggctatcttg actgtatcat agggcggaac cagcaggtcg
     6661 aattccaggg aatactgaac cgcggttgcg attaca
//
LOCUS       DAOSPX010000171         6680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10578_length_6680_cov_5.324830, whole genome shotgun sequence.
ACCESSION   DAOSPX010000171 DAOSPX010000000
VERSION     DAOSPX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10578_length_6680_cov_5.324830"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1464)
                     /locus_tag="PLZ75_08945"
     CDS             complement(142..1464)
                     /locus_tag="PLZ75_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOS72557.1"
                     /translation="MNQRIIRLIFWLPVLILLNDNVYSQVRDSSVMSNEYFKFWNAEI
                     QSKIDRDIERNRKADAILRLNIKPGTNVKVEQISHDFLFGGNIFLFGDLKTFEKNARY
                     ENTFGTLFNAATIPFYWKTLEPEKGKPRYEAGSSYEYRRPPPDPVVAFCESKGINMNG
                     HAIIYGMRKWAHPVWMPEDRKAMEPIFEAHVQELAERYRDRLQRWDVVNEPIDQANRG
                     LMPDDYTYKTFCWAMKYFPEEVQLNLNDCDMHSPINLFRRYVEIIRNLTERGIRIDNT
                     GVQMHIFNPDESRRIADGTDVLTPQKLFEILDCLKDAERPIHISEVTVSAPDNTEQGS
                     KIQAEITRNLYRLWFSYPEVMGITWWNVVDGGAAPGEPSYSGIYDKEMNPKPVYYILN
                     DLINNQWKTNLIVAADKNGTISFRGFKGNYLVSWKEKGKGKAIEYSLK"
     gene            complement(1496..2920)
                     /locus_tag="PLZ75_08950"
     CDS             complement(1496..2920)
                     /locus_tag="PLZ75_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOS72558.1"
                     /translation="MSEAYWKLWNPEVQAKIDRDIDKYRKADAVCILKDVSPGTTVTV
                     EQLSHDFKFGAHIFNYNQLGTRERNKRYKDLYGTLFNSATIAFYWKKFEMQPNRPRFR
                     EEYWDTEEYWNKSSEPKNEPHWRRPSTDQVVEFCESKGIRLHGHTIIWGNQRWSYPDW
                     MFEQFCPPDEKEKLTKLSKEDIASMTPAQIGELAPVYFKEMEKLFDKRITEIAEYYKG
                     RLHSWDVVNESATDFKGNSITGDPMTKSSYGFMPGDYIYKAFVTANKVFPENVLLNIN
                     DYANNENYKNQVTDLLSKGCRIDIMGSQMHLFDPQQCLDIAAGKPVESPQQVWDKMEI
                     ISKANLPIHLSEITITAPGDNEQGREIQAVITRNLYRIWFSIEKMMGITWWNVVDDCG
                     APGEPTTSGLFTRNMEPKPSYYALDMLINHEWKTKMNVKTDNAGILKFRGFRGLYRVM
                     WKDETGQSHELEFYLKNDGDGLKM"
     gene            complement(3079..5142)
                     /locus_tag="PLZ75_08955"
     CDS             complement(3079..5142)
                     /locus_tag="PLZ75_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOS72559.1"
                     /translation="MSHLLDGPAGNHGFVKVEKGRFVTEAGTIRFNATNLTGPANFPD
                     RSQADSIAARLARFGFNCIRLHYMDSPYGNFLHKEMSSILKEDPKTQRDLDSDQLDKM
                     DYLISALKKRGIYVNMNLHVARWWDERDGFTGKDRRPAFDKGLDNFEPRMIELQKEYA
                     KKLLSHVNPYTGLAYTDDPCVAMIELNNENALFRQYMTGGIDRLPEPYASEFRNQWNS
                     WLKRKYKSTESLKKAWMSDTTVIGLKAGMKAPTNLIGEKENLENGTVATLVAGNPAFA
                     QAKKDFFQFMIDTERSYWYGLYKYLKNDLKVKSVVSGTQLGYSSPFVQADLDYIDIHA
                     YWCHPSPVNKDWRIANKPMVNTMSNILSLSAQRIHDKPYTCSEYNHPFPNQYGAEGQA
                     MLRAYGRFQGWDGVFEYTYNHQPDFNPTDNNYFFSIIARTDVLAHMQACAAIFLRGDV
                     SEGKNTVIAPVNYEKYFSDLVRTNEIGANLRSAGLDMKLTLLHKTAVDLTGAEGSDVL
                     SYNIPAGQKVFKSDTDELEWNIEKENAGYFTVNTANTKLFTGFPEGRVIKLGDVSLEV
                     GQTRLGWATISLVSQNATGFGNEGKASILLAATGLSQNKDMVIEKYEDHKIHVSDWGT
                     GPTYVEGIHATIIIPANAAKTKCYSLDPSGNRMHEVKVNKTKGGTRIVISPRYKTVWY
                     EIEVL"
     gene            complement(5359..>6680)
                     /locus_tag="PLZ75_08960"
     CDS             complement(5359..>6680)
                     /locus_tag="PLZ75_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOS72560.1"
                     /translation="QARQSPEWIRNGIMYQIQPRAFTPEGNLKAATVRLQELKRLGIT
                     IVYMCPVFVSDDDMDLQFWSPRQMASKMDNPRNPYRMKDYYHVDPEYGTDDDLKNFVS
                     EAHRLGIKVMLDMVYLHCGPKAVFLKDNPDFIKRDPSGKVLNAAWRFPALNYENSELR
                     EYLWKNMEYWIRDFNVDGFRCDVADQIPIDFWETARDRLEKIKSDIGMLAEGSRPQNT
                     VKAFDLNYSFSWFRAVRDVYQNGQPASSLRKEYEKRLSTHPNGTMFIHYIDNHDIAND
                     SYYNRIEEVWTTKGANAAIVMTFTLDGVPMIYNGQEVADTARHSIFGRLPVKWELGNT
                     PVGKERFALFQKLIKTRLTESSLIKGKVIWLDNDAPDAVISYARELNGQQIISVINLS
                     GKPISVKLKDTSAKKAKPLFMAMAKGNLSKGFNLKGYGYWVGKSSGK"
BASE COUNT         1782 a         1489 c         1255 g         2154 t
ORIGIN      
        1 aacaaaaaat tttcccgatt ttacacaaaa cagggacttt atggacagac actaactaaa
       61 ctccttcact agtgtctggt taaaaatccc ataatgataa ttggttttta tcggaaaatt
      121 tgctgactaa gttgacaatg actacttaag actgtattct atcgcctttc cctttccttt
      181 ctctttccag gaaaccaggt aattgccctt aaaaccacga aaactgatag ttccgttctt
      241 atctgcagca actatcagat ttgttttcca ttgattatta ataagatcgt ttagaatata
      301 atacacgggt ttaggattca tttccttatc ataaattcct gaataagatg gttcaccggg
      361 ggcagcgcca ccgtccacaa cgttccacca ggttattccc attacttctg gatagctgaa
      421 ccaaagtcgg tacaggttcc tggtgatctc cgcctgtatc ttagaaccct gttcggtatt
      481 atctggtgca cttaccgtga cctcgctgat atgaattggc ctttcagcat ctttaagaca
      541 gtcaagtatt tcaaataatt tctgtggagt aaggacatct gtcccatcag ctatcctgcg
      601 tgattcgtcc ggattaaaaa tatgcatttg tactcctgtg ttgtcaattc ttattccccg
      661 ctcagtgaga tttcggataa tctcaacata tcgcctgaag agatttatcg gggaatgcat
      721 gtcacaatcg ttaagattta attgaacctc ttcaggaaaa tacttcatag cccagcagaa
      781 ggttttatat gtgtaatcat cgggcattaa tcccctgttg gcctggtcaa ttggttcatt
      841 aacaacatcc cagcgttgca accgatctct gtatctttct gctagttcct gcacatgtgc
      901 ttcaaaaatt ggctccattg ctttcctgtc ctcaggcatc catacaggat gggcccattt
      961 gcgcatcccg tagatgattg catgaccatt catgttgatc ccttttgatt cgcagaaagc
     1021 aactacagga tcaggtggcg ggcggcggta ttcataggaa cttcctgcct catatctggg
     1081 ttttcccttt tcaggttcaa gggttttcca gtaaaatggt atagtagctg cattaaagag
     1141 agtgccgaag gtgttttcat atctggcatt tttttcaaat gttttaagat ctccaaataa
     1201 aaatatattg ccaccaaaca ggaaatcgtg acttatctgt tctactttca catttgtccc
     1261 gggttttata ttcaacctga gaattgcgtc cgctttcctg ttcctttcaa tatccctgtc
     1321 aatcttagac tggatttcgg cattccagaa tttgaaatat tcattgctca tgacagagct
     1381 gtcccgtacc tggctgtaaa cattgtcatt caataatatt aaaaccggca gccagaagat
     1441 cagtcttata atcctttgat tcattatcag gtatattgtg ttatgattaa aagttctaca
     1501 tctttaaacc atcaccatca tttttgagat aaaattccag ctcatgcgat tgaccagtct
     1561 catctttcca cattacccga tacaatcccc tgaaaccacg gaattttaat attccggcat
     1621 tatctgtttt gacattcatt tttgttttcc attcatggtt tatcagcata tcaagtgcat
     1681 aataagaagg ttttggttcc atattgcgtg taaacaggcc ggaagttgtc ggttctccgg
     1741 gtgcgccaca atcatcaact acattccacc atgtaatgcc catcattttt tcgatgctga
     1801 accagatcct gtaaaggttc cttgtaataa cagcctgtat ctcccgaccc tgttcattat
     1861 cgccgggagc tgtaatggtt atctcgctaa gatgaatggg aagattcgct ttagagatta
     1921 tttccatctt gtcccagacc tgttgtggcg attcaacagg ttttcctgca gcaatatcaa
     1981 gacactgctg aggatcaaaa agatgcattt gtgatcccat aatgtcgata cggcatcctt
     2041 tagataaaag gtctgttacc tggttcttat aattctcatt attagcataa tcatttatat
     2101 tgagaaggac gttttccggg aagactttat ttgctgttac aaatgccttg tagatgtaat
     2161 caccgggcat gaaaccgtaa gagctttttg tcatagggtc accggtaata ctgttccctt
     2221 taaaatcagt cgcgctctca ttaactacat cccagctatg caggcgccct ttgtaatatt
     2281 ctgctatttc tgtgatcctt ttgtcaaaga gcttttccat ctctttgaag tagacaggag
     2341 caagctctcc gatctgtgca ggcgtcatac ttgcgatatc ttcctttgac agttttgtaa
     2401 gtttctcctt ctcatcggga ggacaaaact gttcaaacat ccagtcggga taagaccatc
     2461 tctggtttcc ccagattatg gtatggccat gaagccggat tcccttactt tcacagaatt
     2521 caacaacctg atcagttgaa ggtcttctcc agtgaggttc attcttgggt tctgaagact
     2581 tgttccagta ttcttcagtg tcccagtatt cctccctgaa ccttggccgg ttcggctgca
     2641 tttcaaattt tttccaataa aatgcaattg ttgcggaatt aaatagtgtc ccgtacagat
     2701 ccttgtatct cttgttcctt tccctggttc ctagctgatt gtaattaaaa atgtgagcgc
     2761 cgaacttaaa atcatgtgaa agctgttcaa cggtaacagt cgttcccgga gaaacatctt
     2821 taagaataca aactgcatcg gctttccggt atttgtcaat atcccggtca attttagcct
     2881 gaacttccgg attccagagt ttccagtatg cttcactcat aacactattt acagcagatg
     2941 ttttctgatt cctggttttt ccggccttct gtgaaccgat ctcacctccg aaaagacata
     3001 agcaaaaaaa taaaaaaata aaactcaatt tcataacatc attattaatc tgtttaattc
     3061 atatatgaga acggatcatt acagaacttc aatctcgtac catactgttt tgtaccgggg
     3121 actgataact attctggttc cgccttttgt tttatttact tttacctcat gcatcctgtt
     3181 acccgaggga tctaatgaat aacattttgt tttggcagca ttggccggta taattattgt
     3241 cgcgtgaatt ccttcaacat aggttggccc tgttccccaa tctgaaacat gtattttatg
     3301 atcctcatat ttttcaatga ccatatcctt attctgtgat aatcctgttg cggctagcaa
     3361 aatgctggct tttccttcat tcccaaaccc ggtagcgttc tgtgatacaa gtgatattgt
     3421 ggcccagcca agacgtgttt gtccaacctc aagggaaacg tctccgagtt taattaccct
     3481 gccctcaggg aagcctgtga agagttttgt gtttgctgta tttacagtga agtatccggc
     3541 gttctctttt tcgatattcc attcaagctc atcagtatca cttttaaaaa ccttttgacc
     3601 ggcagggata ttataagata aaacatcaga tccttcagca cctgtgaggt caacggctgt
     3661 tttatgaagc agtgtcagtt tcatatcaag cccggcagat ctcagattag ccccaatttc
     3721 atttgtcctt acaagatcag aaaagtattt ttcgtagttg acaggagcta taactgtatt
     3781 tttcccttca ctgacatcac ccctgaggaa tattgccgcg caggcttgca tgtgagcaag
     3841 tacatctgtt ctggcgatta tactgaaaaa ataattattg tcagtcggat taaaatctgg
     3901 ctggtgattg taagtgtatt caaatacccc gtcccaccct tgaaaacggc cgtatgcacg
     3961 taacattgcc tgaccttcag caccgtactg gtttggaaaa ggatggttat attcgctgca
     4021 agtataaggc ttgtcatgaa tacgctgagc tgataatgac aatatattac tcattgtatt
     4081 aaccatcggc ttgttagcta tcctccagtc tttatttaca gggcttggat gacaccagta
     4141 agcatgaatg tcgatataat ccagatcagc ctgaacaaag ggggagctgt aaccaagctg
     4201 agttcctgaa acaacagatt tgactttcag gtcatttttc agatatttat acaatccgta
     4261 ccaataggat ctttccgtat caatcataaa ctggaagaaa tccttttttg cctgagcaaa
     4321 ggcaggatta cctgcaacaa gtgtggcgac agtaccattc tcgagatttt ctttttcccc
     4381 gataagattc gtgggagctt tcatgcctgc ttttagccct attactgtgg tatcagacat
     4441 ccatgctttc ttcagtgatt ctgttgattt atacttcctt ttaagccagg aattccattg
     4501 attcctgaat tcagatgcat acggctcagg aagtctgtca atacctccag tcatatattg
     4561 ccggaaaagg gcattctcat tgttaagttc tatcattgcc acacaaggat catcagtata
     4621 tgccagtcct gtatatggat taacatgact gagaagtttt tttgcatatt ctttctgaag
     4681 ttcaatcatt cgcggttcga aattatcgag gcctttgtcg aaggcagggc gcctgtcctt
     4741 gccagtaaat ccgtcgcgtt cgtcccacca tcgggcaaca tgcaggttca tgtttacata
     4801 gattcctctt ttcttcaacg ctgaaataag gtaatccatt ttatcaagct ggtcagaatc
     4861 aagatcacgt tgagttttgg gatcctcttt cagaattgaa gacatttcct tatgcagaaa
     4921 attgccataa ggtgaatcca tatagtgcag ccttatgcaa ttgaaaccaa accttgcaag
     4981 ccttgcagca atactgtcag cctggctccg gtctggaaag ttggcaggtc cagtcaggtt
     5041 ggtggcattg aaccttatgg ttcctgcctc agtaacaaat cgaccctttt caaccttcac
     5101 gaagccatga ttgcccgcag gcccgtcgag cagatgagac atatttgtaa cattatcttg
     5161 tgcatcatat gaaataagga aaggaaacat ggattcagat tccttaactt cctttttatt
     5221 gatacacccc gaaagtatgg taaatgttag tagacaaaag aataatatcg gcataggatg
     5281 attttgcctg aaagctgtaa ttgcaataat taggttcatt tcactgattt ttaaggtttt
     5341 ttatgaagtt cgttgaagtt atttcccgga tgactttccc acccagtaac catatccttt
     5401 aagattaaag cccttgctta gattaccttt tgccatagcc ataaacaggg gttttgcctt
     5461 tttggctgat gtgtctttga gctttacgct gatcggtttc cctgaaagat tgatgactga
     5521 gataatctgc tgaccgttta actcacgtgc atatgatatt actgcatcgg gagcatcatt
     5581 gtcgagccag ataactttgc ctttaatcag tgaagattct gtcaaccttg ttttgatcag
     5641 tttctgaaat aatgcaaacc tttctttccc aacgggtgta ttaccaagct cccattttac
     5701 aggaagtctt ccgaaaatgc tgtgacgggc tgtatctgca acctcctgcc cattgtaaat
     5761 catcgggaca ccatcaagtg taaatgtcat tactattgca gcattggctc cttttgttgt
     5821 ccacacctct tcaatacgat tatagtagct gtcattggca atatcgtgat tatcgatata
     5881 atgaatgaac atagttccat ttggatgtgt tgataacctc ttctcatact ccttcctgag
     5941 agatgaggca ggctgaccat tctgataaac atctctgact gctctgaacc aactgaaact
     6001 ataattaaga tcgaaggctt tcactgtatt ttgtggacgt gaaccttcgg caagcatacc
     6061 aatatcagac ttgattttct caagtctgtc ccgtgccgtt tcccagaagt ctattgggat
     6121 ctgatcagca acatcacaac gaaaaccgtc aacattaaaa tctcttatcc agtattccat
     6181 attcttccac aggtattccc tcagctcaga attttcataa ttcagtgcag ggaatctcca
     6241 ggctgcatta agcacctttc ctgacggatc tcttttaata aaatccggat tgtctttcaa
     6301 aaaaacggct ttcggaccgc aatgtaaata aaccatatcc agcataacct taattccaag
     6361 cctatgagcc tctgaaacga agttcttcag atcatcgtct gttccatatt ccggatccac
     6421 atgatagtaa tccttcatcc tgtagggatt acggggattg tccatttttg aagccatctg
     6481 tctgggactc cagaattgca ggtccatatc atcatcagaa acaaaaacgg gacacatata
     6541 aacaatggtt ataccaagcc ttttaagttc ttgcaagcgg acagttgcag ctttcaggtt
     6601 gccctcagga gtgaaagccc ttggctggat ctggtacatt attccattcc tgatccattc
     6661 aggtgactgt cttgcctgtc
//
LOCUS       DAOSPX010000172         6645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10651_length_6645_cov_3.916388, whole genome shotgun sequence.
ACCESSION   DAOSPX010000172 DAOSPX010000000
VERSION     DAOSPX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10651_length_6645_cov_3.916388"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLZ75_08965"
     CDS             <1..364
                     /locus_tag="PLZ75_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOS72561.1"
                     /translation="HLPLSEYIAASHVAAFYIASGVRGMERGTMSSTRDEDGNEIYAD
                     LELMRLALPRRVFTLSQVEYTIDRLNWLYKNRELVGGLRFVEEPPVLRFFTGRLEPVS
                     DWPERLAEAYRKDFGDTL"
     gene            473..2977
                     /locus_tag="PLZ75_08970"
     CDS             473..2977
                     /locus_tag="PLZ75_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOS72562.1"
                     /translation="MSQVILSQPATSWYFPDDVTFNGKIITPEQFAGHQIGEWHLSHD
                     KLYYYMQELARSSDRALWEEYARSHEGRPLGHLIISSPENIRNLERLRLQHISLADPE
                     KSSGTDISNAPVFIKLGYGIHGNESSSQNASVLVAYYLTAGEGPSIDKLLRNIVVLID
                     PALNPDGMQRHSTWVNFSRSLNDNPDPRSWEFSENWPGGRSNHYMFDLNRDYLLLQHP
                     ESVGRVAAFYRWRPDIVTDHHEQGPNATFFYQPGIPTRNNPLTPSENQQLTAEIGKFH
                     EKFLNRIGTLYVTEESYDDFYIGKGSSYPDIHGSVGILFEQAGVKGHLRETSNMLLSF
                     PYTIRNQVAVSLSTLEAGLAMREKLLNSLAGFYREAAALADKHPVKAYIFTEPDDKGR
                     LSEFIRILKSHQIKVYGLSRDAVINGISYSAGASYIVPLKQNEHRFVRSLFEPVKEFT
                     DSTFYDISTWILPMSFNIRYAAITQTREAEALRGKEIREVPLAEGKLLGPEDPFAYLF
                     EWKEYLAPAAPFELQNAGIRTRVAKAGFAYRNSELNHAFGCGTIMIHANDQPVSRTEL
                     KKIILSAAEKFRLTVHGVSTGFTEEGIDLGSSDFVVLEKPSVALIAGDGINSLDAGEI
                     WHMLDTRFRMPVTMIRPAAFDNADLNRYNVLVIAGSPALGPRGIEKIMSWNRQGGTII
                     GYKGGNNWLAANKLAEIEYIPPAESVLKEGIYAHRAMNNQVRQIPGSIFSVKLDLTHP
                     LCYGYTGDIVPVMKTGTAAVKKVGDIYANPAVYTSDPLLSGYCTRENTERLKGSSFVS
                     VHRGRIISIYDNTNFRAIWYGTNKIFLNAVFFGQLL"
     gene            complement(3053..3973)
                     /locus_tag="PLZ75_08975"
     CDS             complement(3053..3973)
                     /locus_tag="PLZ75_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase"
                     /protein_id="HOS72563.1"
                     /translation="MRIFRGFEEVRGIRNPVVTTGSFDGVHIGHKTILNRLRMLATKH
                     EGESVLITFHPHPRKVLYPDTAGKDLKLINTQEEKLELLRKAGLDNVIIVEFTREFSR
                     VTSEEFVRDYLHSILKARVIVVGFNHHFGFNQEGDYRHLWNWRKKYDFEAEEIPEQEV
                     QHETVSSTKIRKAIREGYIQRANAYLDHYYLVMGMVTESALPGIADKVHFVRMPVTEE
                     SKLLPSPGLYAVSLVSENAASKAMAFIAQTDGSGQELFINIFENEESFPGERISVLFH
                     KKIHGALSPDDLHFEYRLVTARDEISELIY"
     gene            complement(3987..4610)
                     /locus_tag="PLZ75_08980"
     CDS             complement(3987..4610)
                     /locus_tag="PLZ75_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOS72564.1"
                     /translation="MKLPGKTVERLSEYRRTLLGCLEEKKNFIFSHELAARLHITAVQ
                     VRRDLMLIGYSSVLRKGYDVRELIETIGTIIDSDVTLNVAIIGIGNLGRAVAGYFRGK
                     RSKLSLVASFDTDPHKVNKVISGVRCYPATEMEKIIGELDIRIAIITAPADSAREIAA
                     EAVRCGIKGIMNFTTISLVVPSWVYLEEYDMITSIEKVAYFVKENKV"
     gene            complement(4807..5943)
                     /locus_tag="PLZ75_08985"
     CDS             complement(4807..5943)
                     /locus_tag="PLZ75_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS72565.1"
                     /translation="MAVLKVLVIDDEPGIRSGVTRILRDFQVTYPFMDEDYTFEMYEA
                     PTGEQGIEMLEKDKPDIILLDNKLPGMQGVEVLEYIHNKNYDIMVAMITSYASLDVAV
                     KATRDGATDFIPKPFTPQELKSSIENITKQLYLRRITRRLTEEGKQIRYQFLSVLSHE
                     LKAPLNAVEGYLRMMQGREAGDRISDYTDHIDRSLQRIQGMRNLIMDLLDFTKIRLEK
                     KEGRIRTLDLAEIARGAIVTVQPYAIQMEVTVSLDVRSDAFIEADPADMDIIFNNLVS
                     NSVKYNKPGGRADIIIDSDDKEVVIRFADGGIGISEEDIPDLFTEFVRIKNEKTRNIT
                     GSGLGLSIVKKIVQLYFGTITVESTPDVGTVFTVRLPKNSSSVT"
     gene            complement(5947..6342)
                     /locus_tag="PLZ75_08990"
     CDS             complement(5947..6342)
                     /locus_tag="PLZ75_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS72566.1"
                     /translation="MKKSDFTILVADDDPDYLYQTVFNLNKAGYKTITAESQAEAEAI
                     IERFRPDLAIFDLMMESDDSGFILCYKIRKKYPDVPVILATAVSRETGMSFSVDTERE
                     KSWIRADRYLEKGIRAEQLDQEIMKLLKI"
BASE COUNT         1717 a         1655 c         1478 g         1795 t
ORIGIN      
        1 ccatctgcct ctgtcggaat atattgccgc atcccatgtt gcagcatttt acattgcttc
       61 gggagtgagg ggaatggaaa ggggtaccat gagcagcacc cgtgatgagg atggtaatga
      121 gatatatgcc gatcttgaac tgatgaggct ggcattgccg agaagggtat ttaccctttc
      181 ccaggtggaa tacactattg accgtcttaa ctggttgtac aaaaacaggg aactggtggg
      241 cggattaagg tttgtggaag aacctccggt actgaggttt ttcaccggca ggctggagcc
      301 tgtcagtgac tggcccgaaa gactggctga agcatacaga aaggatttcg gcgacactct
      361 ttgaaaaagg attgcacagc ctgtctttct gaatcatttt ttcctaaatt gcaggatcaa
      421 cattcattat caagcaatga ggatcataac agctttaatt ccggctcttc tgatgagcca
      481 ggtaatcctg tcccagcctg ccacttcctg gtattttccc gatgacgtga cctttaacgg
      541 gaagattatc acaccggaac aattcgcggg tcaccagatc ggggaatggc atctgtctca
      601 cgacaagctt tactactaca tgcaggagct cgcccgctcc tccgacaggg ccctgtggga
      661 ggaatatgcc aggagccatg agggcaggcc gcttggtcac ctcattataa gctcacctga
      721 gaatatcagg aaccttgaac ggctcaggct gcagcatatc agtctggctg atccggaaaa
      781 atcctccgga acggatatca gcaatgcacc ggtttttata aagcttggct atggtattca
      841 cggcaatgaa tccagttccc agaatgcatc cgtactggtt gcctattacc tgactgcagg
      901 ggaaggtccg tccattgata aactgttgag gaacatcgtg gtactgattg accctgcact
      961 taacccggat ggcatgcagc gccactcaac atgggtcaat ttcagccgca gccttaacga
     1021 caacccggat ccccgctcct gggaattcag cgaaaactgg ccgggcggaa gaagcaatca
     1081 ctacatgttc gacctgaaca gggactacct tctgctgcag catcccgaat cggtaggacg
     1141 cgtggcggcc ttttacagat ggaggcccga cattgttacc gaccatcacg aacagggtcc
     1201 gaatgcaaca tttttttacc agcccgggat cccgacccgc aacaatccgt taacaccctc
     1261 agaaaaccag cagctgacgg cagaaatcgg gaaattccac gaaaagttcc tcaaccggat
     1321 cggaaccctt tatgttacgg aagaaagcta tgacgatttc tatatcggaa aaggttcatc
     1381 ctaccccgac attcacggat cggtcggcat tctttttgaa caggccggag tgaaaggaca
     1441 tctccgcgaa acttccaaca tgctcctgtc ctttccctat accatccgga accaggttgc
     1501 agtatccctt tcaacccttg aagcaggact ggcaatgagg gaaaagctcc tgaacagcct
     1561 ggcgggtttt tacagggaag cagcggcact ggctgacaag catcctgtga aagcctatat
     1621 tttcaccgaa cctgacgaca agggaaggct ttctgaattt atccgcattc tgaaaagcca
     1681 tcagataaag gtctacggtc tctcaaggga tgcagtcata aacggaatca gctacagtgc
     1741 aggcgcctcc tatattgttc ccctgaaaca gaatgaacac aggtttgtaa gaagcctgtt
     1801 tgaacccgtt aaggaattta cagacagtac tttttatgac atttccacct ggatacttcc
     1861 catgtccttc aacatccggt atgcagcaat cacacagacc agggaagccg aagcactaag
     1921 ggggaaggaa atcagggaag ttccgcttgc cgaaggaaag ctgctgggac cggaagatcc
     1981 ctttgcatat ctctttgaat ggaaggaata ccttgctccc gcagcgcctt ttgaactgca
     2041 gaatgcagga ataaggacaa gggtcgcaaa ggccggattc gcatacagga acagtgaact
     2101 gaaccatgca ttcggctgcg gcacaattat gatccatgca aatgatcagc cggtcagcag
     2161 gacagaactg aaaaaaatca ttctgtcggc agctgaaaaa ttccgcctga ctgtacacgg
     2221 tgtcagcacc ggtttcacgg aagagggaat tgaccttgga agcagtgatt ttgtggtgct
     2281 tgaaaaacca tcggtggccc tgattgccgg ggatggaata aacagcctgg acgccggtga
     2341 aatatggcat atgctcgaca cccgcttccg gatgccggtg accatgatac gtccggcagc
     2401 atttgacaat gccgacctta accgctataa cgtgcttgtc attgcaggct cccccgcact
     2461 gggtccccgg ggaattgaga aaatcatgtc atggaacagg cagggcggga ccatcattgg
     2521 ctacaagggc ggcaacaact ggctggctgc aaacaaactg gctgagattg aatacatacc
     2581 cccggcggaa tcagtcctga aggaaggaat atatgcacac agggccatga ataatcaggt
     2641 acgccagata cccggttcaa tattttcagt aaaacttgat ctcacccacc ccctgtgtta
     2701 cggatatacc ggggatatcg tcccggtaat gaaaacagga acagctgcag taaagaaggt
     2761 cggggatatt tacgctaacc cggcagttta tacctcagat ccgctgctca gcggctactg
     2821 caccagggaa aataccgaac ggctgaaagg aagctcattt gtttcggttc accgcggcag
     2881 gatcatttcc atttacgaca ataccaactt cagggcaata tggtacggta caaacaagat
     2941 attcctgaat gcggtattct tcggacagtt actgtaaaca gcatccggaa gaaaggaggc
     3001 ttaagaagtg cacagaaagt atatagaaag tatctgttcc gctgccttaa tgtcagtaaa
     3061 tgagttcgga tatttcatcc cttgcagtta cgaggcggta ttcaaagtgc agatcatccg
     3121 gcgacaaggc tccgtgtatc ttcttatgaa aaagcactga gattctttct cctgggaatg
     3181 attcctcatt ctcaaatata ttgatgaata actcctgtcc ggatccgtca gtctgggcga
     3241 tgaaggccat tgcctttgaa gcagcatttt ccgacacaag ggacactgca taaagcccgg
     3301 gtgaaggaag gagcttgctc tcttcagtca cgggcatgcg gacaaaatgt actttgtctg
     3361 caattcccgg caaagcgctt tctgtcacca ttcccataac aagataataa tgatcaagat
     3421 atgcatttgc cctctgtata taaccttccc ttatggcttt acggatcttt gtggaactga
     3481 ccgtttcgtg ctgtacttcc tgttcgggaa tctcctcggc ttcaaagtca tactttttcc
     3541 tccagttcca gagatgcctg taatcccctt cctggttaaa tccgaaatgg tgattaaatc
     3601 ccaccactat tactctcgcc ttcaggatgc tgtgcaggta atccctgaca aactcctcac
     3661 tggtgactct tgaaaactcc ctggtgaatt ccactattat cacattgtca agtccggctt
     3721 tcctgagaag ttcaagcttt tcctcctgtg tgttaataag tttgaggtcc tttccggcag
     3781 tgtcgggata aagcactttc ctgggatgcg gatggaaggt gatcagaacc gattctccct
     3841 catgcttcgt ggcaagcatc ctgagccggt taagaatggt tttatggcct atatgaaccc
     3901 cgtcgaatga tccggttgta acaacgggat ttctgattcc ccttacttct tcaaatcctc
     3961 tgaaaattct cattatgaat atgattctaa accttgtttt ccttaacgaa ataagcaact
     4021 ttttctatgg aggttatcat atcatattct tccagataca cccatgaagg aacaacaagc
     4081 gatatggtgg tgaagttcat gattcccttt atcccgcagc ggacggcctc agcggcaatc
     4141 tccctggctg aatctgccgg ggccgtgatt atagctatcc tgatgtcaag ctcacctatt
     4201 atcttttcca tttccgtggc aggatagcat ctgactccgg aaataacctt gttcaccttg
     4261 tgcggatcgg tgtcgaagga ggctacaaga ctgagttttg atctttttcc ccggaagtat
     4321 cccgcaacgg ctctgccgag attgccaata cctattatcg caacattcag cgtaacatcc
     4381 gaatcaatga tggttccgat tgtctcgata agttccctta catcatatcc ctttcgcagc
     4441 accgaggaat aacctataag catcaggtcg cgccttacct gaaccgctgt aatatgcagt
     4501 cttgccgcga gttcatggga aaaaataaaa ttcttctttt cttccagaca tccgaggagt
     4561 gtccttctgt attcgctgag ccgttcaact gttttacccg gtaatttcat agaaaaacat
     4621 tattactctt ttattgaaga tttactgatt tgcataagcc taccggattt aaagggcaaa
     4681 gcttcattcc tgcagtttgt tttcacattt gcaggtattt gtccacagca ctcacaggat
     4741 aaggatttca tacagtcatc attaattcca gaaaaaatcc gcggagcatg aaaatctcac
     4801 attgcttcag gttacggatg aggagttctt tggtaatctt acagtaaaaa ccgttcctac
     4861 atccggggtg ctctccactg tgattgtccc gaaataaagc tgaacaatct tctttactat
     4921 ggaaagcccc agtccgcttc ctgttatatt tcttgttttt tcatttttta tccttacaaa
     4981 ttcagtaaag agatcgggaa tatcttcctc gctgatacct attcccccgt ctgcaaacct
     5041 gataacaacc tccttatcgt ccgaatctat aatgatgtcc gccctgcctc cgggtttatt
     5101 gtattttacc gaattggaaa caagattgtt gaagattatg tccatatcgg cgggatctgc
     5161 ctctatgaaa gcgtctgatc tcacatccag actgacggtg acttccatct ggatggcata
     5221 gggctgcact gtaactattg cccccctggc tatttcagca agatcaaggg ttctgatcct
     5281 gccttccttc ttttccagac gtatcttggt aaaatccagc agatccatga tcaggttcct
     5341 cattccctgt attctctgca atgatctgtc aatatggtcg gtataatcac ttatcctgtc
     5401 gcccgcttcc ctcccctgca tcatcctgag atagccctcc acggcattga gaggtgcttt
     5461 cagctcatgt gaaagaacgg aaagaaactg ataccttatc tgtttcccct cctcggtgag
     5521 gcggcgggtt attcttctga gatatagctg tttggttata ttttcaatac tcgatttaag
     5581 ctcctggggt gtaaaaggct tgggaatgaa atcggtggct ccgtcacgcg tagccttgac
     5641 cgccacatca agggaagcgt atgaggttat catcgcaacc atgatgtcat aattcttgtt
     5701 gtgaatgtat tcaaggactt ccactccctg catgccgggc agtttattgt cgagaagaat
     5761 aatgtccggc ttgtccttct caagcatttc aataccttgc tcacccgtgg gtgcttcata
     5821 catctcgaat gtataatcct catccatgaa gggataggta acctgaaaat ccctgagtat
     5881 cctggtgaca ccggagcgga ttccaggttc atcatctatg acaagtactt tcaatactgc
     5941 catttcctat atttttaaca gtttcattat ttcctgatcg agctgctcgg cacgtattcc
     6001 cttctcaaga tacctgtcgg ccctgatcca tgatttctcc ctctccgtgt caacactgaa
     6061 cgacatgccg gtttccctcg aaacggcagt tgcaagtatc accggaacat cgggatattt
     6121 tttccttatt ttgtagcaca gtataaaacc actgtcatca ctttccatca taagatcaaa
     6181 aatggcaaga tcaggacgga agcgttcaat tattgcttcc gcctcagcct gactttctgc
     6241 cgtaatggtt ttgtaaccgg ccttattcag gttgaaaaca gtctgataga gataatccgg
     6301 atcatcatca gccacaagaa tagtaaaatc actctttttc atctcggtac aaatatcctt
     6361 aaagttttga aaatttcatt ttttatttct tttccggcag gcagtcccct atcccttgcg
     6421 ccggggcagt acaatcttaa atctcgtccc tgtggggccc tttgacggat cattgtccga
     6481 ctcaacactt atctggccct tatgcatctt tacaatgccg taggttgttg ccagaccgag
     6541 cccggtaccg ttaccgagac ttttggtggt aaagaagggt tcaaatattt acaccctgtc
     6601 ctcttccttt attccgcttc ccgtatctcc cacggtaatg cttat
//
LOCUS       DAOSPX010000173         6549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10840_length_6549_cov_4.110564, whole genome shotgun sequence.
ACCESSION   DAOSPX010000173 DAOSPX010000000
VERSION     DAOSPX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10840_length_6549_cov_4.110564"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLZ75_08995"
     CDS             <1..313
                     /locus_tag="PLZ75_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72567.1"
                     /translation="TSNKIIIKTFDNVTYIPNECVHTGVDGIPVVYTKNGFKQIVVLG
                     ESNEKETVIEDGLKPGTVLYLAMPQETNNFRLSGEELLDIIRDRQKERQALNTVRPGI
                     K"
     gene            complement(434..970)
                     /locus_tag="PLZ75_09000"
     CDS             complement(434..970)
                     /locus_tag="PLZ75_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72568.1"
                     /translation="MEKRVSRLKIMLSAMIILAFTIMPFSLDGQTGKVNFSGTWALNA
                     DKSTFGPGPGGQAPQGQRPGGGFGGGSFVAKQEANQLTVERTRGDQTFTSKYFLDGRE
                     SVNSTGRGESKSTASWSADGKTLKIVTTRRFSMGGQDTEMKTTEEWTLSGTNVLTIVS
                     TSSTPGGERKSTMLYDKK"
     gene            complement(1034..3274)
                     /locus_tag="PLZ75_09005"
     CDS             complement(1034..3274)
                     /locus_tag="PLZ75_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOS72569.1"
                     /translation="MKEKAEEKVRKEAAEKVQDGTTGKADGSISSGAGGNDRGGGMKR
                     RNFVRLLGGGIAVLGSGIFIYFRPWKAPDLLKGAPQPRRSLTKDYNAFLHIQPDGMIN
                     CYTGKIEMGQGVITSLPLMMADELNVPLDRIKIVMGDTELCPYDAGTWGSLTTQQFGP
                     AMRAALAEARAVLVEMASEKLNVPVNRLEVRDGIVVDTANPATGISYGDLARGKKLEK
                     YLDVKPPVEDHTEFTWVTKPAMRADSYEKVTGRAIYTGDIKLPGMVFARILRPPSHGA
                     SLKSVDVSAAEKMPGVQVVRDGDFIAVLSEDRDRADEAIVRIDAEYSFDELQVNDRTL
                     FDWLVNADSDIKVLRSTGNIEQGRKLSDNIFESEFHDGYCAHASIETHTALARFEGDK
                     LTVWAATQTPFGLQDALVQEFGMPLEKVRVIVPFVGGGFGGKIAFQQGVEVARIAKLS
                     GKPVMLEWTREEEFFYDTFHPASVIRIKSGTDKSGLIKMWDYHVYYGGTRGSETTYDV
                     PHSRTTSYSESKKSGQIHPVLTGAWRAPNNNTNTFARESQIDIMASKAGIDPLEFRLK
                     NLKDENMIACLKTAADKFGYKPAKAPSGRGIGIACGFDAGTWVAAIAQVSVDRSTGRI
                     KVERVVAAQDMGLCVNPQGAVLQMEGCVNMGLGYALREEVFFEGGKVFTNGFDNYEIP
                     KFSWIPEIECVILDRRDKAPKGGGEPVIIAIGAIIGNAVFDATGARLYRMPMSPERVL
                     EAIKKI"
     gene            complement(3271..3753)
                     /locus_tag="PLZ75_09010"
     CDS             complement(3271..3753)
                     /locus_tag="PLZ75_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOS72570.1"
                     /translation="MEEKLSFTLNGKKTEVLIDTSRTLLWVLRNHFGLTGTKYGCGTG
                     FCGACTVLIDDEPFRSCMLPVADVAGKRVVTIEGLEKNGSLHPVQKAFIEHDALQCGF
                     CTPGMILTAVGLLMKNPSPTRQQIIEGMEDNLCRCGAHNRIIDAVETAAKEIKEGRKS
                     "
     gene            complement(3755..4348)
                     /locus_tag="PLZ75_09015"
     CDS             complement(3755..4348)
                     /locus_tag="PLZ75_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS72571.1"
                     /translation="MSRIYAVILAAGASARMGSMKMLLPFQGMTVIEKVIGNVLASGL
                     KNILVVTGSGKEDLVRVIGKMPVEWCFNEFWKDGMLSSVQCGIRSLPEDCEAILVCLG
                     DQPMTDPETLKKLVRAHGNTDRGIIIPVFRKKRGHPILIRKKYFSEIEKLDPEKGLRM
                     LAHEFPDDVLEVESDNPGILKDIDTPDDYRNEITQTC"
     gene            complement(4414..5505)
                     /locus_tag="PLZ75_09020"
     CDS             complement(4414..5505)
                     /locus_tag="PLZ75_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HOS72572.1"
                     /translation="MIDNIYFRLLRLAPVISNPVLVTITETEGSTPQKAGNSAIFSNR
                     GLIAGTVGGGLAERRVQELAIEYAGTGKSSFYHSSFDNTSDDPDEAICGGKVTMLIDA
                     DPLRHLDVYRDMEKTIMEEKPGVLATWVSQHEDLSVGIQRFWIKDRPGDGLPPQVADI
                     LRPLVSEILASQDSNGYVKTELEIPGEKSKHILFLEPVFPLPKLVIAGAGHIGKALSH
                     LGRLTGFEVTVIDNRKEFANSDNLPEAGRIIVKDIGQAMAEIDKTPDTYIVIVTRGHG
                     CDADALRPCIGSEAGYVGMIGSRAKVAKMRSDFISEGWATEDQWKRIHSPIGLEIKSE
                     TVQEIAVSIMAQLILVKNSRKSRQDLSDH"
     gene            5665..>6549
                     /locus_tag="PLZ75_09025"
     CDS             5665..>6549
                     /locus_tag="PLZ75_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOS72573.1"
                     /translation="MKGGSTIKKTAYIAFFLFMIACQPPVNNTGTREYKVHPWLEELT
                     IAGLRKAYQDGRYTISEVVKAYIERINKIDMAGPELNSIISINPDALSISAELDREMR
                     EGRLRGPLHGIPVILKDNIDTGDRMPTTAGSNALAASFASRDSYIAGKLREAGAVIIG
                     KANLSEFANFRARTSSSGWSAVGGQTKNPYVLDRNPCGSSSGSGVAVSANLCMAAIGT
                     ETNGSIVCPSNHNGIVGIKPTVGLLSRRGIIPISYTQDTPGPMARTVEDAAAFLGAMT
                     GVDPEDLKTLESEGKSHRD"
BASE COUNT         1598 a         1768 c         1367 g         1816 t
ORIGIN      
        1 cacaagtaac aaaataatta tcaaaacatt cgataacgtt acctatatac ccaatgaatg
       61 tgttcatacc ggtgttgacg gcattcccgt ggtctataca aaaaacggtt ttaaacagat
      121 cgttgtacta ggggaatcca atgaaaagga aactgttatt gaagacggat taaagcccgg
      181 aacagtgctt tacctggcca tgccgcagga aacaaacaat ttcagactct cgggagagga
      241 actccttgac attatcaggg acaggcagaa ggaaagacag gcactgaaca ctgtaagacc
      301 cggcataaaa taaactcata tagaagccgt tcaggcagaa cggctgaaac tgactgtacg
      361 cttcagatca ataaaataag gggctcccgc aggagcccct tattttattc agcctgtttc
      421 agttccctcc tgcttatttt ttatcataca gcattgttga tttcctttcc ccgccggggg
      481 ttgaggaagt tgatacaatg gtaagtacat tcgtgccgct gagtgtccat tcttcagtgg
      541 ttttcatctc cgtatcctga ccgcccattg aaaacctgcg tgttgtgaca attttaaggg
      601 ttttcccgtc agccgaccat gaagcagtcg atttactctc tcctcttcct gttgagttga
      661 cgctttccct gccatcaagg aaatatttgg aagtaaaggt ctggtctcct cttgttcttt
      721 ccacagtaag ctggtttgcc tcctgttttg caacaaagct gccgccgcca aatccgccgc
      781 ccggcctctg accctgggga gcctgaccac ccggacccgg tccgaacgtg cttttatcgg
      841 cattcaatgc ccatgttccg gaaaaattaa ccttgcctgt ctgtccatca agggaaaaag
      901 gcataatggt gaaggcaaga attatcattg ccgataacat gatcttcagt ctgctaactc
      961 ttttttccat tttttcatga tttaattggt tgataacatt tgtatagaca aagtatcagg
     1021 ccggattatt ccatcatatt ttttttattg cttccagcac cctttcagga ctcattggca
     1081 tacgatacag tctggcgccg gttgcatcaa aaacagcatt gccgataatc gcaccaatgg
     1141 ctataatcac gggttcgccc cctcccttcg gggctttatc cctcctgtcg agaatcacac
     1201 attcaatttc agggatccat gagaactttg gtatttcata gttgtcaaac ccgtttgtga
     1261 atacctttcc tccctcaaaa aatacttcct ccctcagggc atatcccagt cccatgttaa
     1321 cacagccctc catctgcagc acagcaccct ggggattgac acataatccc atatcctgtg
     1381 cagccactac cctttccact tttatcctgc ctgttgacct gtccacgctg acctgggcaa
     1441 ttgctgccac ccaggttccc gcatcgaagc cgcaggcaat tcctattccc cttccactgg
     1501 gtgcttttgc cggtttatag ccgaatttat ctgccgcagt cttaaggcag gctatcatgt
     1561 tttcatcctt caggtttttc agacggaatt caagaggatc aatacccgct tttgatgcca
     1621 taatatctat ctgcgactct cttgcaaaag tgttggtatt gttgttcggt gcccgccatg
     1681 caccggtaag tacagggtga atctggccag attttttact ttcgctgtag ctggtggtcc
     1741 ttgaatgagg cacatcatag gttgtttcgg atcccctggt gcctccgtag taaacatgat
     1801 agtcccacat ttttatcagg cccgatttat cggttccgga tttgattctg attactgaag
     1861 ccggatgaaa ggtatcataa aagaattcct cttcccttgt ccattcaagc attaccggct
     1921 ttcctgacag ttttgcaatt cttgctacct caactccctg ctgaaaggct atcttgccgc
     1981 cgaatccgcc tccgacgaat ggcacaataa cccttacctt ttcaagaggc atgccaaatt
     2041 cctgtaccag tgcatcctga agtccgaaag gcgtctgggt tgccgcccac accgtaagtt
     2101 tgtcaccttc aaacctggca agcgccgtat gcgtttcgat ggaagcatgg gcacagtagc
     2161 cgtcatgaaa ttccgattca aaaatgttat ccgacaattt acggccctgc tcaatatttc
     2221 ccgtgctccg caggaccttt atatccgaat cggcattcac cagccagtcg aacagtgtcc
     2281 tgtcatttac ctgcagttca tcaaagctgt attcggcatc tattctgact atggcttcat
     2341 ctgcccgatc acggtcctca gacagtaccg cgataaaatc cccgtcacgg accacctgta
     2401 ctcccggcat cttttccgcg gccgaaacat caacggattt caggctggca ccatgtgacg
     2461 gaggtctaag tattcttgca aataccatcc ccggcaattt aatatcaccc gtatatatgg
     2521 ccctgccggt tactttctca tatgaatccg ccctcatcgc cggtttcgtc acccaggtaa
     2581 attccgtatg gtcttcaaca ggaggtttca catccagata tttttccagc tttttgcccc
     2641 tggcgagatc tccgtaactt attccggttg ccggatttgc agtgtcaaca acgattccat
     2701 ccctgacttc caggcggtta acgggaacat tcagcttttc cgatgccatt tcaaccagta
     2761 ctgcccttgc ctcagccagg gcagctctca tggcagggcc gaactgctgt gttgtaaggg
     2821 aaccccatgt tccggcatca taaggacaaa gctcagtatc tcccataaca atctttatcc
     2881 tgtccagggg aacattcagt tcatccgcca tcatgagcgg aagggatgta ataacaccct
     2941 gtcccatctc aatctttccc gtatagcagt ttatcatccc gtccggctga atatgcagga
     3001 aggcattata atcctttgtc agggaacgcc tcggctgggg agcgcccttc aacaggtcag
     3061 gcgctttcca tggcctgaaa taaataaata tcccactgcc cagtacggct atgcctcctc
     3121 ccagcaatct gacaaaattc cttcttttca ttccgccgcc cctgtcattg ccgcctgctc
     3181 cggaagatat gcttccgtca gcctttcccg ttgtaccgtc ctgcaccttt tccgctgcct
     3241 ctttccttac tttttcctct gctttttctt tcatgacttc ctcccttctt ttatttcttt
     3301 tgctgccgtt tcaacagcat caataatacg gttgtgtgca ccacagcggc acaggttatc
     3361 ctccatgcct tcaatgatct gctgcctggt gggtgacgga tttttcataa gcaggcccac
     3421 tgcggtaagt atcatgcccg gggtacagaa tccgcactga agtgcgtcat gttcaataaa
     3481 tgccttctga accggatgga gactgccatt tttttccaga ccttcaatag tcaccactct
     3541 tttccctgca acatcagcca ccggaagcat gcacgacctg aaaggctcgt catcgataag
     3601 caccgtacag gctccgcaaa aaccggttcc gcatccgtac tttgttccgg tcagtccgaa
     3661 atgattccgc aaaacccaca gcagggtccg ggaagtatca atcagaactt ccgtcttttt
     3721 tccgtttaag gtaaaactta acttctcttc catattaaca agtttgggtt atttcatttc
     3781 tgtagtcatc cggagtgtca atatccttca ggatccccgg attatccgat tccacctcaa
     3841 gaacatcatc aggaaattca tgtgccagca ttctcaatcc tttctcagga tcaagttttt
     3901 caatttcact gaagtatttc tttctgatca gtatggggtg tccgcgtttt ttccggaaaa
     3961 caggtatgat tattcccctg tcagtattac cgtgggcccg gaccagtttt tttagcgttt
     4021 ccgggtcagt catcggctga tcacccaggc aaacaagtat ggcttcacaa tcttcaggca
     4081 atgatcttat cccgcattgc accgatgaca gcatcccatc cttccagaac tcattgaagc
     4141 accattccac cggcattttt ccgatgaccc tgacaaggtc ttcctttccg gatcctgtga
     4201 caacaagaat atttttcagt ccggaagcca ggacattccc tatgactttt tcaataacag
     4261 tcataccctg aaaaggcagg agcattttca tggaacccat tcttgccgag gctcctgcgg
     4321 caagtattac agcatatatc ctgctcattc cgcgtattgt ttcagatatt ggtccttatc
     4381 tgctgttaac aagcatttcc ggccggattt tcattaatga tccgataaat cctgtctgct
     4441 tttcctgctg tttttcacca gtatcagctg ggccataatg ctgaccgcta tttcctgcac
     4501 tgtttccgac tttatttcca gacctatggg tgaatggatc ctcttccact gatcttccgt
     4561 tgcccatcct tcgctaatga agtcggaacg catctttgcc acctttgccc tgctccctat
     4621 catacccaca tacccggcct ccgatcctat gcacggccgc agggcatccg cgtcacagcc
     4681 atgccccctg gtaacaataa caatataggt gtcaggtgtc ttgtcaattt cagccattgc
     4741 ctgacctata tcctttacga ttattctgcc cgcttccggc agattgtcgg aattggcaaa
     4801 ttctttccgg ttatcaataa ctgtcacctc aaacccggtc agccggccca gatgggaaag
     4861 agccttcccg atatgtccgg ctcccgctat tacaagtttt ggcaggggaa atacaggttc
     4921 caggaataaa atatgttttg atttctctcc gggaatttcc agttctgttt ttacataacc
     4981 attgctatcc tgtgatgcaa gaatttcgga taccaggggt ctcaggatgt ccgctacctg
     5041 aggcggcaga ccgtctcccg gtctgtcctt aatccagaaa cgctgaatgc cgacggataa
     5101 atcctcgtgc tgactcaccc atgtagcaag gacacctggc ttttcttcca tgatggtttt
     5161 ttccatatcc ctgtaaacat ccagatgcct gaggggatca gcgtctatga gcatggtgac
     5221 ctttccgcca cagatggctt catccgggtc atcgcttgta ttgtcaaatg aagaatgata
     5281 aaaacttgat ttacctgtac ccgcgtattc aattgccagt tcctgtaccc gtctctcagc
     5341 caggcctcct cccacagtgc cggcaatcag tccccggtta ctgaaaattg ctgaatttcc
     5401 cgccttctga ggcgtggaac cttcagtctc cgtgatggta accagtacag gattactgat
     5461 gaccggagcc agccggagca gtctgaaata gatattatct atcattacaa taatactctg
     5521 cgaactgaaa ctgcaattta gatcccggaa aaaaatacaa acaccgctgt cagcccgata
     5581 aaacaaatat attaaaattg aggaataaat aataattttg tctgaagcgg catatttttc
     5641 ttttaaacaa ttaaacatca cactatgaaa ggcggatcaa ccataaaaaa aacagcatat
     5701 atagctttct tccttttcat gattgcatgt cagccacccg taaacaatac cggcaccagg
     5761 gaatacaagg tgcatccctg gctggaagaa cttacaattg ccggtctgcg aaaagcttat
     5821 caggatggcc ggtacacaat aagtgaagtg gttaaggcat atattgaacg catcaataag
     5881 attgacatgg ccgggccgga gctcaattcc attatttcaa tcaacccgga tgcactttcc
     5941 atttcagctg aactggacag ggaaatgcgg gaaggcaggc tcaggggtcc gcttcacggc
     6001 attccggtca ttcttaagga taacatagat acgggagaca ggatgccaac tacagccggt
     6061 tcaaatgccc tggcagcatc atttgcctca cgtgacagtt atattgcagg caaactgcgt
     6121 gaagccggtg ctgttattat cggcaaggca aacctgagcg agttcgcaaa cttcagggcc
     6181 cggacttcat ccagcggctg gagtgctgtg ggaggtcaga ctaagaatcc gtatgtgctt
     6241 gacaggaatc cctgcgggtc aagctccggg tcaggagttg ctgtttccgc caatctgtgc
     6301 atggcagcaa tagggacgga aaccaacggt tccatcgttt gtccttctaa ccacaacgga
     6361 attgtaggaa taaaacccac tgtcggcctc ctcagccggc gtggaataat accaatttca
     6421 tatacccagg acactccggg ccccatggcc aggacagttg aagatgcagc tgctttcctg
     6481 ggggcaatga ccggggtcga tccggaagat ctgaaaactc tggaatcaga ggggaagtcc
     6541 caccgggat
//
LOCUS       DAOSPX010000174         6528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10874_length_6528_cov_3.393326, whole genome shotgun sequence.
ACCESSION   DAOSPX010000174 DAOSPX010000000
VERSION     DAOSPX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10874_length_6528_cov_3.393326"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLZ75_09030"
     CDS             <1..857
                     /locus_tag="PLZ75_09030"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOS72574.1"
                     /translation="RKGSNLNKLIYINIAVFIIVTAISILSFLLSNPSITSKTVDLIA
                     VPSSFDKLVLRPWSVITYMFTHKDIWHILFNMLWLYWFGMIFLEFLDQRKLVAVYLLG
                     GLSGALVYILSFNVFPAFREIVHESVAIGASASVMAIVIAIAAYVPNYTIHLFLIGRV
                     KIIYVALVIFLLTSFVDFSVNSGGKLAHIGGALFGYFYSVNLRKGRDAGRGINRIIDF
                     LTTIFKAPEKKKIIHKKPKTDYEYNRIKSERQEKINMILDKISKGGYDSLTKEEKDTL
                     FSESQKKN"
     gene            878..1957
                     /locus_tag="PLZ75_09035"
     CDS             878..1957
                     /locus_tag="PLZ75_09035"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOS72575.1"
                     /translation="MRKIIYKILLSVNLIFAFTLLLSYLAVHISPARFALPALFGLAY
                     PYILLANILLAITWALLLKYEALISVIVIAAGYGHLTNYLRLSRPDSETAGTFKVLSY
                     NIRLFNAFEKGNKGSEKEILEFLKKQEADIICLQEFYGRGNPDQKVREIRDYLGNDYQ
                     SHLKVIGSGRNRFYGVVTFSRFPVAGRGEIIHPHSPALTIFTDVIISRDTFRIFNNHL
                     HSFGLKSMEKSFIEEVIQQEEGNTLGGMKDLSLSLKNGFVRRAQQAELLKGQINLSAY
                     PVIVAGDFNDTPVSYTYTKIRKGLGDAFVNSGYGAGFTYRGNYPANRIDYIFYDNAFK
                     CKYFDIVKIRYSDHYPVVAYFTLSR"
     gene            complement(2031..2975)
                     /locus_tag="PLZ75_09040"
     CDS             complement(2031..2975)
                     /locus_tag="PLZ75_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOS72576.1"
                     /translation="MIFPSLPDIVTAHDRIRPFIHHTPVFTSGQLNKLFGCELFFKCE
                     NFQKAGAFKFRGATNAVRLLPDEEKQKGVVTHSSGNHAAALALASAMNGVKAYIVMPG
                     TAPEVKKNAVAGYGAEITFCEPTAEAREKAARSLAEKTGAVLIHPYDNFNVICGQGTA
                     ALELMSEINDLDIIVAPVGGGGLLSGTSTAARAVRKDIIIAGAEPLNANDAWKSFTTG
                     VLCPSVSPDTIADGLLTSLSELTFSIIRNNVDQILTVTEEAIKECMLLIWERMKIIAE
                     PSAATVLAAVKENPGFFRGKRTGLIISGGNVDFRKLPF"
     gene            complement(3142..5097)
                     /locus_tag="PLZ75_09045"
     CDS             complement(3142..5097)
                     /locus_tag="PLZ75_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOS72577.1"
                     /translation="MKKNSKNFLGGIDIKKPSLTGLIITLGIVYGDLGTSPLYTVKAI
                     VNTAGEFNELLIFGALSCVFWTLTLLTTIKYIIITTHADNKGEGGIFALFALIRKKSA
                     WAAIVTLIGAGALLADGVITPAITVTSSIEGLRLFNPVMPVVPIVLIIFGLLFSVQQF
                     GTNVVGILFGPIMVLWFLMLGILGFSQLIHYPRILIALNPVFALRFLVEYPGGFILLG
                     AVFLCTTGAEALYSDLGHCGRTNIRISWIFVKTMLLLNYLGQGAWLLRNYTPGTQAMP
                     FFSMMPEWFLLPGIILSTAAAIIASQALVSGSFSLVSEAVSLNFWPPIRILQPTLTRS
                     QIYIPFVNWSLMFMCSLVVIFFRNSSNMEAAYGLAITIAMLMTTSLLARYLYQKNIDI
                     KLIVLFLAVFLAIETSFLIANLHKFRHGGWFTLILGTAYGLVMYGWYFGRKIKNRYLT
                     FVNLENYKQLFIDIKADNSIPRTASNLVYIIKANRMEQIESKIIFSIFKKRPKRADKY
                     WLLHVDIVNEPYRFEYKVTYLIPDVLIRIDFHLGYKIGKSINLYFREVVEDLVQSGEI
                     KMLSGYNFLKKYEFQGDFKFIITERIIPPDFNLSKTENFILALNSLIKHLSLPAESAL
                     NLDPTCTEVEKIPILVEKPLTHRARRS"
     gene            complement(5407..>6528)
                     /locus_tag="PLZ75_09050"
     CDS             complement(5407..>6528)
                     /locus_tag="PLZ75_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOS72578.1"
                     /translation="QQGPATVIIEGMAVGMASTWIPVVTIVIGIIAAYGFSGGFQNFA
                     MGVYGIGFAAVGMLSTLGITLATDAFGPIADNAGGNAEMSELPKEVRERTDALDGLGN
                     TTAATGKGFAIGSAALTALALIAAYMEEVRLWLGRVIDKAPEGFKQIGDTLFYKSVIP
                     QVQDGQVAVHIGSATIRDFTSAYDITLLNPIVLGGIFIGAMMAFLFSSMSMKAVGRAA
                     GNMVEEVRRQFREIPGIMDGSGKPDYARCVAISTAGAQREMIIPSLMAIAVPLLIGVL
                     FGVAGVVGLLAGGLSAGFVLACMLNNAGGAWDNAKKFIERGNYGGKNSEAHKASVVGD
                     TVGDPFKDTSGPALNILIKLMSMVSVVLAGLTVAWSMLG"
BASE COUNT         1877 a         1505 c         1381 g         1765 t
ORIGIN      
        1 acaggaaggg cagcaatctc aacaagctga tctatataaa tatagctgta tttatcattg
       61 ttaccgccat ttcaatactg tcattccttc tcagtaatcc gtcgatcaca tcaaaaaccg
      121 ttgacctgat cgcagtaccc tcatcatttg acaagcttgt tctgagacca tggtcggtaa
      181 taacatatat gttcacacac aaggatatat ggcatatatt gttcaatatg ctctggcttt
      241 actggttcgg aatgatattt ctggaattcc ttgatcagag aaagcttgtt gcggtttatc
      301 tgcttggagg tttaagcggg gcccttgtat atatattatc attcaatgtc ttccctgcct
      361 tcagggaaat agtccatgaa tcggtcgcaa taggtgcatc cgcatcggta atggcaattg
      421 tgattgcaat agcagcctat gtgcccaatt atacaatcca cctctttctt atcggaaggg
      481 tgaagataat ctatgttgcc ctcgtgattt ttcttctgac ctcatttgtg gatttctcgg
      541 tcaattcggg agggaagctt gcccatatcg gaggggccct gttcggctat ttctattcgg
      601 tcaatctcag gaaaggaagg gacgccggca ggggaatcaa caggataatt gattttctca
      661 ccacaatctt caaggctccg gagaaaaaga aaatcattca taaaaaaccg aaaaccgatt
      721 atgaatacaa caggattaaa tccgaaaggc aggaaaaaat caacatgatt cttgacaaga
      781 tttccaaggg cggttatgac agtctgacaa aagaagaaaa agacaccttg ttcagtgaaa
      841 gccaaaagaa aaactgataa acatatcagg ctttaatttg agaaaaataa tctataagat
      901 cctcctcagt gtgaatctga tttttgcatt cacactcctc ctttcctacc tggctgtcca
      961 tatcagtcct gcgagatttg cactgccggc cctctttggc ctggcctatc cctacatact
     1021 tcttgcaaac atcctgctgg ccataacctg ggccctgctt ctgaaatatg aagcccttat
     1081 atctgttatt gtaattgcag ccggttatgg tcacctgaca aattacctga ggctttcaag
     1141 gccggacagc gaaacggcgg gtacctttaa ggtgctgagt tacaatatca ggctttttaa
     1201 cgcttttgag aaaggaaata agggatcaga aaaagaaata ctcgagttcc ttaagaagca
     1261 ggaagcggat attatttgcc tccaggaatt ttacggcagg ggaaatcctg atcagaaagt
     1321 aagggagatc agggattacc tgggcaacga ttaccagtcg cacctgaaag tcatcgggag
     1381 cggcaggaac aggttctacg gagttgtcac attcagcagg tttcccgtgg caggcagggg
     1441 tgagatcatt catccgcatt caccggctct gaccatcttt accgatgtta tcatcagcag
     1501 agacacattc aggatattca acaaccatct tcattcattc ggactgaaaa gcatggaaaa
     1561 atccttcatc gaggaagtaa ttcagcagga agaaggaaat accctggggg gaatgaagga
     1621 tctgtccctc agccttaaga acggctttgt caggagagcg cagcaggcag agctgctgaa
     1681 gggacaaatc aacctgtcgg catacccggt tatcgtcgcg ggagatttca atgacacccc
     1741 cgtttcctat acctatacaa agatccggaa aggccttggt gatgcattcg tgaattcagg
     1801 atacggtgcc ggttttacct acaggggaaa ctacccggcc aacaggatag actacatctt
     1861 ctatgacaat gcttttaaat gtaaatattt cgatatcgta aagataaggt attctgacca
     1921 ctatccggtc gtagcctact tcacactttc cagataagag atccgtaaag gggccttctg
     1981 tagctttctg ccgcgcatga ctgcagggga taaaggcggg cgacaaagga tcagaagggc
     2041 agtttccgga aatcaacatt tccgccgctg attataagac ccgtcctttt tcccctgaaa
     2101 aatcccgggt tttcctttac ggccgcgagc acggttgctg ctgaaggttc agcaattatc
     2161 ttcattctct cccagatcag cagcatacat tcttttattg cttcttcagt cacggttagg
     2221 atctggtcaa cattattacg gattattgag aaagtgagct cactcaggga ggtaagaagg
     2281 ccgtcggcta tggtatcagg actgactgaa ggacagagga caccggtagt gaatgatttc
     2341 catgcatcat ttgcattcag gggttccgca cccgcaatta ttatatcttt tctgacagcc
     2401 ctggcagcag tggacgtgcc gcttaagagt ccgccccccc ctaccggtgc gacaatgata
     2461 tcaaggtcat ttatttcgct catcagctcg agggcagcag ttccctgtcc gcaaataaca
     2521 ttaaaattat catagggatg aatcagaact gccccggtct tttcagccag ggaccgggca
     2581 gctttttcac gcgcctcagc tgtgggctca cagaatgtga tctccgctcc gtagcccgca
     2641 acagcatttt tctttacttc aggtgccgtt ccgggcatca cgatatatgc ctttacaccg
     2701 ttcattgccg acgccagggc aagagctgcc gcatgattcc ccgatgaatg ggttacaacg
     2761 cctttctgtt tttcctcatc cgggaggagc cttactgcat tggttgctcc cctgaattta
     2821 aaagctcccg ccttctgaaa gttctcacac ttgaaaaaaa gctcacagcc gaacagttta
     2881 ttcaactgac ctgaggtgaa taccggagta tgatggataa agggtctgat tctgtcatgt
     2941 gcagttacaa tatccggaag agacggaaag atcatctggc tctgatttta aacctgttat
     3001 ttttatttcg ataattacct cttaaattta aaaattattt tggacaattt cctcatagcc
     3061 tgaatcttga aatatgacaa tggttcatga atccattctt tttgaaaatg acgcaggaac
     3121 tgtttttacc tcacctggtc atcaacttct cctggccctg tgagttaaag gcttttcaac
     3181 caatatcggt attttttcga cttcagtaca tgtgggatca aggttaagtg cactctctgc
     3241 cggcaggctc agatgtttta taagtgaatt taaagcaaga atgaagttct cggtttttga
     3301 caagttaaaa tccgggggaa ttattctttc ggtaattata aatttaaaat caccctggaa
     3361 ttcatatttc ttcaggaaat tgtatccgct taacatttta atttcacccg actgaaccag
     3421 gtcttccact acctctctga aatagagatt tattgatttc ccgattttat atcccagatg
     3481 aaagtcgatt cttataagaa catccggtat caggtaagtt actttatatt caaaacgata
     3541 cggttcattc acaatatcca cgtgtaataa ccagtattta tcagcccgct ttggtctttt
     3601 tttaaaaatt gaaaaaataa tttttgattc aatctgttcc atccggtttg cttttattat
     3661 atagaccaga ttagatgcag ttctgggaat actattatcc gccttgatat caataaacaa
     3721 ttgtttgtaa ttctcaagat taacaaaggt tagataacgg tttttaatct ttcttccgaa
     3781 ataccatcca tacattacga ggccataagc tgttcccagt atcagcgtga accagcctcc
     3841 atgtctgaac ttgtgaagat ttgcaatcag aaagctggtt tcgatagcga gaaatacggc
     3901 caggaacaac acaataagct taatatcaat atttttctga tacaaatacc gggctaataa
     3961 tgaagtggtc atcaacatgg caattgtgat ggcaagacca taggcagcct ccatatttga
     4021 agaatttctg aagaagatca ccaccaggct gcacataaac atcagcgacc agtttacaaa
     4081 cgggatataa atctggctcc ttgtcagagt aggctgaagt attcttatcg gcggccagaa
     4141 gttcagggaa acggcttcgc ttacaagtga gaatgatccg cttacaagag cctggcttgc
     4201 aatgatggca gcagctgttg aaagaatgat acccggcaaa aggaaccatt ccggcatcat
     4261 tgaaaagaaa ggcatagcct gtgtccctgg agtataattc cttaataacc atgcaccttg
     4321 tccgagataa ttcagcaaaa gcattgtttt gacgaatatc caggatatcc tgatatttgt
     4381 acgtccgcaa tgacctagat ccgaatacag ggcttctgct cctgttgtac aaagaaaaac
     4441 tgcacccagg agaatgaatc cgccgggata ttccacaaga aaccttagtg caaatacggg
     4501 attcagtgca atcagaatac gtgggtaatg aatcaattgc gaaaatccca ggatgccgag
     4561 cataagaaac cataaaacca ttatgggtcc gaataatatt cccactacgt tggtgccaaa
     4621 ttgctgaaca gaaaaaagca atccgaaaat tatcaggaca attggaacta ccggcattac
     4681 gggattaaac agtctcaggc cttcaattga tgaagtaact gttatggctg gtgttatcac
     4741 cccgtcagcc agtaatgctc cagcaccgat cagagtaaca attgcagccc acgcggattt
     4801 ttttcttatc agggcaaaaa gggcaaaaat tcctccctcg cccttattat cagcgtgcgt
     4861 tgtaataatg atatatttga tcgttgtaag taatgtaaga gtccagaaaa cacaggaaag
     4921 ggcaccgaat atcagcaatt cattgaattc acctgcagta ttgacaattg cttttacagt
     4981 atacagcggg ctggttccga ggtccccata aactattccc agggtgataa tcaaacctgt
     5041 aagggatggc ttcttaatat caattccgcc cagaaaattc ttcgaatttt ttttcacgaa
     5101 aaatggttta aaccccgaac aattataaga ttatcaataa tccttcgcaa caaacacgtt
     5161 tttaaatgtt gtatacccat aaaaaagctt gcaacggtgg tccggtgaat aaacggcttt
     5221 atccgtccat gggcacttct gatatctgta acggagggca ggatcatatg aaatggtttt
     5281 ttcaaaatta tgaatttatg cgcaatccgg agtcatcgca atcctgctta aataccagcc
     5341 tgcctgtcaa aaaaaaggct gtcccggaac gcgggacagc cttttgtatt ttatagactc
     5401 aacagatcag ccaagcatgc tccatgcgac cgtaaggccg gcaagaacaa ccgaaaccat
     5461 tgacatcagt ttgatcagaa tgttgagggc gggacccgat gtatccttga agggatcacc
     5521 aaccgtgtca cccacaaccg atgccttgtg agcctcactg ttttttcctc cgtaatttcc
     5581 tctttcgatg aatttcttgg cattatccca ggcaccaccg gcattgttaa gcatgcatgc
     5641 aagaacaaat cccgcactca ggccgcctgc aagaaggccg acaacacctg ccactccgaa
     5701 aagaacacct ataagcagcg gaacagctat agccatcagt gaaggaatga tcatttccct
     5761 ctgggcacct gctgttgata ttgcaacaca gcgtgcatag tcgggcttgc cgctgccatc
     5821 cataatacca ggaatttcac ggaactggcg tcttacttcc tcaaccatgt ttcctgcggc
     5881 acgtcccact gccttcatgc tcattgaact gaagaggaat gccatcattg cgccgatgaa
     5941 aatacctccg agaacaatgg gattaagcag ggtaatatca tatgctgaag taaaatccct
     6001 gatggtggca cttcctatgt ggactgcaac ctgaccatcc tgtacctgcg gaatgacgga
     6061 tttgtaaaag agggtatcgc caatctgctt gaatccttca ggagctttgt caataaccct
     6121 gccgagccac agtctcactt cctccatata ggcagcaatc agtgccaggg ctgtaagagc
     6181 ggcagatccg atagcaaatc cttttccggt tgctgcagta gtatttccaa gtccgtcaag
     6241 agcgtctgtc ctttccctta cttccttggg aagttccgac atttcagcat tgccgcctgc
     6301 attatcggct ataggtccga aggcatcggt tgcaagggtt atacccagtg ttgaaagcat
     6361 accgacagcg gcaaaaccga taccataaac acccattgca aaattctgga aaccgcctga
     6421 gaaaccatag gctgcaataa tacctatcac gatggtcacc accggaatcc aggttgaagc
     6481 cataccaact gccatacctt cgataattac agtagccgga ccctgttg
//
LOCUS       DAOSPX010000175         6495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_10957_length_6495_cov_3.930124, whole genome shotgun sequence.
ACCESSION   DAOSPX010000175 DAOSPX010000000
VERSION     DAOSPX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10957_length_6495_cov_3.930124"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLZ75_09055"
     CDS             complement(<1..315)
                     /locus_tag="PLZ75_09055"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147386.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOS72579.1"
                     /translation="MAKTFQKEYYPGIGKIKFEGRESKNPLAFRWYDPEQVVSGKKMK
                     DHLRFAIAYWHSFCGDGSDQFGSPTHFYPWDSVADNDEKIKMRLDAAFEFFTKIGTGY
                     YCF"
     gene            complement(354..1835)
                     /locus_tag="PLZ75_09060"
     CDS             complement(354..1835)
                     /locus_tag="PLZ75_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOS72580.1"
                     /translation="MLLLGLDLGSSAVKASVLDGVTGNCLASAFYPKDEMKIVAQQPG
                     WAEQDPETWWKNIKAAISDCNTQLGERKKDIGAIGISYQMHGLVIVDKSFNILRPSII
                     WCDSRAVDYGEKAFKSLGEEYCLSHMLNSPGNFTASKLAWVKEYEPDTYRRIHKIMLP
                     GDYIALKLTGEAVTTWTGLSEGILWDFAENRVSSELLDHYGFSPDLLPHVMPSFSIQG
                     RVTPGAAREMDLPEGILVSYRAGDQPNNALSLNVFNPGEVAATAGTSGVIYGVTDMKK
                     YDPLSRVNTFLHVNHSPYEPRLGVLLCINGTGILNSWLRRNAGGNVQYTVMNDMAGKV
                     AIGSDGLSVLPFGNGAERMLGNKNIGARILGLNFNIHSNSHLFRAAQEGIVFSFRYGF
                     DIMKSTGIEPAVIRAGASNMFRSSVFREALSCVTGTEIHLYNTDGSVGAARGAGIGCG
                     YYMSAEEAFTGLKSTGVTVPDREKTGKYEEAYHKWLEYLKEVI"
     gene            complement(1930..2955)
                     /locus_tag="PLZ75_09065"
     CDS             complement(1930..2955)
                     /locus_tag="PLZ75_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOS72581.1"
                     /translation="MKNSKEVTIYDVAKVLNLSPSTVSRGLKDHPHIKKETCDRIKVV
                     AREMGYRRNKFASSLRKKSTETIGVVVPRLNSYFMATAIAGMEKITNQLGYGLIISQS
                     QESAKQEIACLSTLYNSRVDGFLISLAYDSADFNYFNTLFSKGIPVVFFDRVAEYNDC
                     MSIVIDNFRAGYEITKHLTEQGCKRIMHLGGNLLRNVYYDRFWGYRRALDEAGLEFDD
                     KMHVINDLSSQSGLDVARSILEMKSRPDGIFTANDTTAVALICELQREGIRIPEDIAV
                     AGFNNEPVSQVIKPNLTTIDYPAREIGEVAATSLINKLRYNENIGFSRVVLRHRLIIR
                     DSSLKKG"
     gene            3300..4547
                     /locus_tag="PLZ75_09070"
     CDS             3300..4547
                     /locus_tag="PLZ75_09070"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS72582.1"
                     /translation="MLIRITNICIALLIPVFCQAQKIASVEVFFSKGTKGLDIPVCID
                     LDKITYLPDSLLVLCKVNGDTEKQIPFQIEQGNPRKLHWIAETGTNSSERHYYELTKG
                     KAANQPRIKADVRDGALLIHMEDRNFLQYWFSTVYPPEGVDTVYKRSAFIHPLWSPHG
                     QVLTRIQPRDHYHHYGIWNPWTHVAFEGDTIDFWNINTRKGTVRFADFVSIQNGSVYS
                     EFDAIHDHVVFKKDGRERIALKELQSVRVYAPENDPGFFIVDFNIQLNCAGDSPVKLL
                     EYRYAGLGWRTTEKWDNKNSMVLTSEGKSRKDADGSKARWCIVQGEIDDDYAGVVMMS
                     FPTNYNFPEPLRIWPENQYNRGDMFANFCPTKDTDWLLEPRHNYVLRYRFLVYNNRIE
                     RDKAENAWEYFANPPEIRIISAK"
     gene            4805..>6495
                     /locus_tag="PLZ75_09075"
     CDS             4805..>6495
                     /locus_tag="PLZ75_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS72583.1"
                     /translation="MKRIILLFLVFAGFSFSLAAQKVVTGKVTDEDLNPLGGVSVIIK
                     GTSIGTLTDADGKFTLTVPGEAKVLSFSFIGMKSHDLEMGTRTNFDVVMSVDVGLLDE
                     VIVIGYGTVKKRDLTGAVTRINADELQTEASANVTSMLRGAIPGLSVGFNITPKGISN
                     AKEMLIRGETSLRSDDSQMADANAPLVVVDGMIYYGDLADINPVDIESFDILKDASSA
                     AIYGARASNGVILITTKKGRPGKPVININTSTGIAYVSPAHYPLMNGEQFIARRKAGF
                     EANERNQIMIGPGYYNSPEDLPSGVTLEQWKAYDGSSAATDLVGVWLNRIGFAAVEID
                     NYKKGRETDFSKYTWQTGLTQDYNLNVSGSSREISYYLSLGYTNNEGIRYNESFNTIR
                     SRLNLEANITDWLKVGTNTQVAFRDESPIVVGRSFYNTPYSSMYEDDGVTLTFSPSGY
                     VNAPNFWYEMVYHDRFIKYNTMNSKIYGTIVFPLGITFTSEFIPRFNWNRNYQSWSTG
                     HPNWAVEGGRALRQNTTIFEWQVNNMLKWNQTFDIHTFDVTLVQNAEKYQYWQDY"
BASE COUNT         1879 a         1494 c         1347 g         1775 t
ORIGIN      
        1 aaaacaataa tatccggtac ctatcttggt aaaaaattca aaggcagcat cgagcctcat
       61 ctttattttt tcatcattat ccgccacact gtcccaggga taaaaatgtg tcggggatcc
      121 gaactgatca ctgccgtcac cgcagaatga atgccagtat gcaattgcaa aacgaagatg
      181 atctttcatt tttttacctg acaccacctg ttcaggatca taccacctga atgccagagg
      241 attctttgat tccctgcctt caaattttat cttccctata ccgggataat actctttttg
      301 aaaagtctta gccatatcac tcagatttta ttttgtttca tttcaccagc tgatcatatt
      361 acttccttaa gatattcaag ccatttgtgg tacgcttcct cgtacttacc tgttttttcc
      421 ctgtcaggta cagtgacccc ggtagatttc aaaccggtaa atgcctcctc tgccgacatg
      481 taatatccgc atcctattcc tgcaccacgg gccgccccga cagatccatc ggtattatac
      541 aggtgtatct ccgttccggt aacgcatgaa agagcttccc tgaaaacact gctgcgaaac
      601 atgtttgacg ctcccgccct tatcacggca ggttcaatcc ccgttgactt cattatgtca
      661 aacccgtatc tgaacgaaaa aacaattccc tcctgggcag ccctgaaaag atgcgaattg
      721 ctgtgaatgt tgaaattgag tccgagaatc ctggctccta tgtttttatt gcccagcatt
      781 ctctccgccc cgttcccgaa aggcaggacc gaaaggccgt cagagcctat ggcaactttt
      841 ccggccatat cattcattac ggtgtactga acattgccgc cggcatttct tctgagccat
      901 gagttaagta ttcctgtgcc gttgatgcaa agcagtactc ccagccttgg ttcatacgga
      961 ctatgattga catgaagaaa ggtattcacc cttgaaaggg gatcatattt cttcatgtcg
     1021 gttaccccgt aaataactcc cgatgttcct gcggttgccg ccacttcacc cggattgaaa
     1081 acattcaggg acaatgcatt gttaggctgg tcaccggcac ggtacgatac caggattcct
     1141 tccgggagat ccatttccct ggctgctccg ggagtaactc tgccctgtat ggaaaaagat
     1201 ggcattacat gaggaagcag atccggactg aatccgtaat ggtcaaggag ctcggaagaa
     1261 accctgttct ccgcaaaatc ccataaaatg ccctcagaca aacccgtcca ggttgtcacg
     1321 gcttctcctg taagcttcag ggcaatataa tcacccggaa gcattatttt atgaatcctt
     1381 cgatatgtat ccggctcata ctccttcacc catgcaagtt ttgaggccgt aaaattaccg
     1441 ggggagttaa gcatatgact gaggcaatat tcctcaccga gggatttaaa tgctttctct
     1501 ccgtaatcaa ctgccctgct gtcacaccat attattgacg ggcgcagtat attgaagctc
     1561 ttgtcaacaa tcaccagacc gtgcatctgg tatgaaatac caatggcccc gatatccttt
     1621 ttcctttccc ccagctgtgt gttacaatcg gatatagcag ctttgatgtt tttccaccag
     1681 gtttcgggat cctgttcagc ccatccgggc tgctgagcca cgatcttcat ctcatccttc
     1741 ggataaaagg ctgaagccag gcagtttccc gtaactccgt caagaaccga ggccttgact
     1801 gcagaactcc caagatcaag tccgagcaaa agcatttctc ctgtcttttt aaatggtttt
     1861 attacttcag tgactaaagt tactgtttta tttaacaaaa ttgctgaagg tttgcctgaa
     1921 actgaagggt catccttttt ttagtgaaga atccctgata attaatctgt gcctcagaac
     1981 tactctgcta aaacctatgt tttcattata ccttaacttg tttatcaggg aagtggctgc
     2041 cacttcccca atctccctgg ccggataatc tatggttgtc aggttgggtt ttatcacctg
     2101 actgacaggc tcgttgttga atcctgcaac ggcaatgtct tccggaatcc ggatcccctc
     2161 tctctgaagt tcacatatca aagccaccgc cgttgtgtca ttggcagtaa atataccatc
     2221 aggtctggat ttcatttcaa gtatgctgcg ggcaacatca aggcctgact gactgctcag
     2281 atcattaatt acatgcattt tgtcatcaaa ctccagtcct gcttcatcca gggcacgcct
     2341 gtaaccccag aacctgtcat aataaacatt gcgcagaagg ttccctccaa ggtgcattat
     2401 ccgtttgcag ccctgttccg taagatgttt tgtaatttca tatcctgccc tgaagttatc
     2461 aatcacaata ctcatgcaat cattatattc cgcaacacgg tcaaagaaaa ccaccggaat
     2521 gcccttgctg aacaaagtat tgaaataatt aaaatcagca ctgtcatagg cgagcgaaat
     2581 aaggaatccg tcaacacgac tgttataaag agttgagaga catgcaattt cctgttttgc
     2641 actttcctgt gactggctta ttataagccc atagcccagc tgatttgtga tcttttccat
     2701 acctgcaatg gctgtggcca taaagtaact gttaagcctg ggcacaacaa cacctattgt
     2761 ttccgtgctt ttctttcgca gactgctggc aaacttgttg cggcggtatc ccatctcgcg
     2821 ggcaaccact tttatcctgt cacaggtctc tttctttata tgaggatgat ccttaagtcc
     2881 gcggcttaca gttgagggag acaggttaag gacttttgca acatcataaa tcgtaacctc
     2941 cttgctgttt ttcattttct gttattgtat ttcaaaatta gtttaaataa aagccttaaa
     3001 aaaattatat aacaaaattt tgcaatcgat tgcgattaat aattttttca tttagcttta
     3061 tatgcccatg acattaaact caggcagaaa agtgatttat taatattgta tttactgtgc
     3121 gcgatattaa gtgctttaat tcaataatca aatccggcat atactacaga aaaaacacat
     3181 ttatactcac actgttacga aacggaaaat tccggaaatg acactgaaaa aatgaacata
     3241 atcaccatga cattcatcac tgattcattt tactgtttcc tgaacacaat caaaacaaaa
     3301 tgctgataag aattacaaac atctgtattg ctctcctgat tccggttttc tgccaggcac
     3361 agaaaattgc ttcagtcgag gttttctttt ccaaagggac gaaaggactg gatattcctg
     3421 tctgcatcga tcttgataag ataacgtatc tgccggacag cctgcttgtg ctctgcaagg
     3481 taaacggaga tactgaaaaa cagattccct tccagatcga acagggaaat ccgaggaaat
     3541 tgcactggat tgcagaaacg ggcacaaaca gctctgaaag gcattattat gaactgacaa
     3601 agggaaaggc ggcaaaccag ccccggataa aagctgatgt gagagacggg gctttactga
     3661 tacacatgga agacaggaat ttcctgcagt actggttcag tacggtatat cctcctgagg
     3721 gggttgatac ggtatacaaa agaagcgcat ttatacatcc cctctggtct cctcacggtc
     3781 aggtgctgac acgcattcag ccccgggatc attatcacca ttacggaata tggaacccgt
     3841 ggactcatgt ggcttttgaa ggagatacga ttgacttctg gaatattaat acccggaagg
     3901 gaactgtacg gtttgcggat tttgtgtcaa tacagaacgg atccgtatat tcagaatttg
     3961 atgccattca tgatcatgtc gtattcaaaa aagacggaag ggaaaggata gccttgaagg
     4021 aattgcagag cgtacgtgtt tatgcccctg agaatgatcc gggatttttc attgttgact
     4081 ttaacataca attgaattgt gccggggata gcccggtcaa gctgctggaa taccgttatg
     4141 ccggacttgg ctggagaaca actgaaaaat gggacaataa aaacagcatg gtacttacat
     4201 ctgaaggtaa atcacgaaaa gatgcggacg gaagcaaggc aagatggtgt atagtacagg
     4261 gtgaaatcga cgatgattat gccggagttg ttatgatgtc attccccaca aattacaatt
     4321 ttcccgaacc tttacggata tggcctgaga accagtacaa ccgtggcgat atgttcgcaa
     4381 atttctgccc cacaaaagat acggactggt tacttgaacc acgccataat tatgttctca
     4441 ggtacaggtt cctcgtttac aataaccgga ttgaaagaga caaggcggaa aatgcatggg
     4501 aatattttgc aaatccgcct gaaataagaa tcatttctgc aaaataaaaa gccatgctga
     4561 aaaacttaaa aaagttacgc aatcgattgc aatataaaat aaaggtatgt aaatttgaat
     4621 tgccgcttta agcccgtttt tatcctgatc ggatttgaag tatttatatt aagctgataa
     4681 tgagttcgtc gaatcagatt ttcatataac tcaatacgta gagtgcactt ttttaaaatt
     4741 tatcatggac ttaacagggg aaaaatcctg acatatattt ttatgaacct tttaatttac
     4801 ctctatgaaa agaatcattc tgttattcct tgtgtttgcc ggattctcct tttctctggc
     4861 agcacaaaaa gtagttaccg gaaaagtaac ggatgaagac ctaaaccccc tgggtggagt
     4921 ttcggtaatc atcaaaggaa caagcatagg caccctgaca gatgccgatg gaaaattcac
     4981 tcttacagtt cccggtgagg caaaagttct gtctttttcc ttcattggaa tgaaatccca
     5041 tgacctggaa atgggtacac ggacaaactt tgatgtggta atgagcgttg atgtcgggct
     5101 tcttgatgaa gttattgtaa tcggctacgg tacagttaaa aagcgcgacc tcaccggagc
     5161 tgtcacacgt ataaatgcgg atgaactcca gaccgaagcc tctgccaatg tgacatcaat
     5221 gctcagggga gccatacccg gattgtccgt aggattcaac atcactccca aagggatcag
     5281 taatgcaaag gaaatgctga tcaggggaga gacctcactg agatcagatg acagtcagat
     5341 ggctgatgcc aatgctccgc tggtggtggt cgacggaatg atatattacg gcgacctggc
     5401 cgatattaac cctgttgaca ttgaatcctt cgatatactg aaggatgcct cttcagctgc
     5461 aatctacggg gcccgtgcat caaacggtgt aattcttata accacaaaaa aaggaaggcc
     5521 gggaaaacct gtaatcaata ttaatacaag cacaggtata gcatatgtct ccccggcaca
     5581 ttatcccctc atgaacggcg aacagttcat agcccgcaga aaagccggct ttgaagccaa
     5641 tgaaagaaac cagatcatga ttggtcccgg atactataac tcacctgaag acctgccttc
     5701 gggtgtaaca ctggaacagt ggaaagccta cgacggatcc agcgcggcaa ccgacctggt
     5761 cggtgtctgg ctgaacagaa ttggttttgc cgccgtggaa atagacaatt acaagaaggg
     5821 aagagaaaca gatttcagca aatacacctg gcagaccgga cttacccagg attataacct
     5881 gaatgtctcc ggcagctcac gggaaatatc ctactacctg tcactgggat ataccaacaa
     5941 tgagggtatc aggtataatg aaagcttcaa taccatacgt tcacgtctca acctcgaagc
     6001 caacattaca gactggctga aagtgggaac caacacccag gttgccttcc gggacgaaag
     6061 ccctattgtg gtgggaagaa gcttttacaa tacaccttat tcctcaatgt atgaggatga
     6121 cggtgtcaca ttaacctttt ccccgtcagg ctatgtaaac gccccgaatt tctggtatga
     6181 aatggtctat cacgacaggt tcataaaata taatactatg aacagcaaaa tctacggaac
     6241 aattgtattt cctctgggaa taacctttac ttccgaattt attccacgct tcaactggaa
     6301 cagaaattat cagtcatggt caacaggaca ccccaactgg gcagttgaag ggggcagggc
     6361 attaagacaa aacactacca tttttgaatg gcaggtcaac aacatgctca aatggaacca
     6421 gacattcgat attcacacat ttgatgtaac acttgtccag aatgccgaga aatatcagta
     6481 ctggcaggat tatat
//
LOCUS       DAOSPX010000176         6445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11073_length_6445_cov_3.940532, whole genome shotgun sequence.
ACCESSION   DAOSPX010000176 DAOSPX010000000
VERSION     DAOSPX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11073_length_6445_cov_3.940532"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..751)
                     /locus_tag="PLZ75_09080"
     CDS             complement(113..751)
                     /locus_tag="PLZ75_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOS72584.1"
                     /translation="MSCKSTGTIFLSVLILFAAEAFLVVRAGKWLVRHDGDIHGDAIV
                     ILMGSIADRVLQAEHVYRQGLADKIIIVKEGMGAYKALESKGVSLISTTKQVSDALVT
                     LGISSGNIVILPGDAVSTQMEAVKVREYCFCRADIDTLLLVTSAYHTRRASMIFESAF
                     RKKGIPVTILCSPSSFTKFNPAGWWKDKEGIQQVLLEYMKIANFLLFDRRKM"
     gene            complement(777..1703)
                     /locus_tag="PLZ75_09085"
     CDS             complement(777..1703)
                     /locus_tag="PLZ75_09085"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS72585.1"
                     /translation="MKNSILLTLVISIFLFSCESKQQDFVSLFNGKDLTNWTLQKQGA
                     FEVIDGEMITRSTGSGTDIYTEKAYSNFILQLEYMLSEAGNSGVFIRRDPSKPGSGFE
                     VQLLAPWTPWRDDLHCTGSLYGHVAVTNRPDETTGKWYRMEIKCDRNMVTVSVNDQIT
                     TMADIDTVKSLAGKPLTGYIGLQGNHAEKPDQFARFRKIRIRDLDAEPDYVTEGFSNP
                     NWEIRYVSNQAATSLGAVMIQSMAVMMSGSDPMLRSEAKQVLFDIVAKTSDPGTSEKE
                     KRTLIREIDKSINSCSSEITSDYLRWLRGMLE"
     gene            complement(1729..3024)
                     /locus_tag="PLZ75_09090"
     CDS             complement(1729..3024)
                     /locus_tag="PLZ75_09090"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72586.1"
                     /translation="MGSNSKINRRKFITRSTLGLMGLPLLSSGLRAVAPSDRVRVAVI
                     GLGSQGRSHMTWFNNLPEVEVAALCDLDKIRLGEARARLQEINPDSRAEVFSDFRRIL
                     DRKDIDAITCATPDHWHAPIAIMAFQSGKDVYGEKPLTYSLAEGQAMLKNLEKHNRVF
                     QLGTQIHAGDNYHRVTELLQSGRLGKIHTVRLWKTGGSPGLGFPPPQEPPETLDWDMW
                     LGPAPYEEYTPVRCHGTYRHFLDYSGGVFADFWCHIADIMFMSLHPKGLYSIDSRGER
                     PFDGIADAPRWIDVDFKFKDLDVFWTTIPPEAPGAEKMHIGAHFEGTGGTLTCDYTSR
                     FIKINNEILTDLPDVPVTLPRSPGHQQNFIDAIKSGKEPESNLHYVREMTIPMHLALI
                     SFRLKRKLVWDSEKEDFVGDRAASYLLSREYRKPWKLSS"
     gene            3109..4116
                     /locus_tag="PLZ75_09095"
     CDS             3109..4116
                     /locus_tag="PLZ75_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOS72587.1"
                     /translation="MIRMAVIYTNLFLLVILLSCKNNTEPDNSKPDRNTFMNPVYPSS
                     MPDPSIIRADDGYFYLYATEDVRNTPILRSSDLVTWNLIGTAFTSETRPSFEPRGGIW
                     APDINYIAGKYVLYYSMSVWGGEWTCGIGVAVSDSPEGPFTDRGKLFRSNEIDVQNSI
                     DQYFFEENGKKYLFWGSFHGIYAVELTDDGLAVREGTEKRFIAGTAYEGACIYKKDGF
                     YYLFASTGTCCEGIKSTYTTVVGRSENLFGPYMDKSGNSMSNNHHEVLISGNDRFAGT
                     GHNSEIITDKKNKSWILYHAVDKINPRGRVLMLDEVQWKDGWPFVEGNTASLKWTRPY
                     F"
     gene            4140..4730
                     /locus_tag="PLZ75_09100"
     CDS             4140..4730
                     /locus_tag="PLZ75_09100"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOS72588.1"
                     /translation="MSPEFRNITAIILAGGKNSRMGGIDKGMLPVKNVPMIAHIAKQL
                     EPFFGEVIIGGRTDIYGFLGYRVIPDISEGMGPLMGIYSCLLASDTDLNFITACDIPD
                     IRIGLVREMLRLSAESDIVIPMGAGNKYEPLHGVYRKSVIPAAAGLLEDKRLRTSDLF
                     EQVKTMFIPFDGREWYHNINTPEDYSSYAKKADDPA"
     gene            4852..6219
                     /locus_tag="PLZ75_09105"
     CDS             4852..6219
                     /locus_tag="PLZ75_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOS72589.1"
                     /translation="MEQRGSPSELGTGNIRKLLLKYAIPSVIAMTASSLYNITDSIFI
                     GHGVGALAISGLAITFPLMNLSAAFGSLVGVGAATLMSLRLGQKDYGTANNILGNVFV
                     LNIILGLIYTVVVLLNLDRILYFFGASDETLPFAHDFMVIISLANIITHLYFGLNAML
                     RATGHPKKSMFTTLFSVLINIILAPIFIFVFRWGIRGAALATVIAQTSMLLWQILHFS
                     NSRNFIHLKKGSFRLKRRIVTESLSIGMAPFMMNSVASIIVLVINTSLIRYGGDLAVG
                     AYGIINRIAFLFIMVVLGFNQGMQPIAGYNHGAGQFMRVNNVLKHTIVLATSVMMVGF
                     AIGELFPRAVASAFTTDQDLIDIVVPGMRIVMMSFPIIGFQMVTSNFFQSIGMPVKSI
                     LMSLSRQVFFLLPCLLIFPRIFGLKGIWYSMPTADVISAVVAAVLLVGQYRKNRLNEI
                     THQEI"
     gene            6230..>6445
                     /locus_tag="PLZ75_09110"
     CDS             6230..>6445
                     /locus_tag="PLZ75_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOS72590.1"
                     /translation="MKENYIITIGRQLGSGGREIGLKLAESLGISFYDKELIRLASEK
                     SGLNEKVFESADEKKRFNIFWGLFGVHS"
BASE COUNT         1673 a         1452 c         1476 g         1844 t
ORIGIN      
        1 ctgaaaagtg gtgaaaagtg gttgtttctg gtttgggacc agagggtcgt cagttcgaat
       61 ctgaccatcc cgacacagac cttcagatcc ggttttttct cctgattccc gtttacatct
      121 ttctcctgtc gaacaggaga aaatttgcaa ttttcatata ttccagcaat acctgctgaa
      181 tgccttcctt gtctttccac catccggcag gattgaattt cgtaaaactg ctcggactgc
      241 agagaatagt gaccggaata cccttcttcc tgaatgccga ttcaaatatc atggacgccc
      301 ggcgggtatg ataggcagaa gttacaagca aaagggtgtc aatatccgcc cgacagaagc
      361 aatactccct tactttaacg gcttccatct gtgtgctgac agcatcaccg ggcaggatta
      421 caatattgcc tgaggaaatt ccaagggtta ccagtgcatc acttacctgc tttgtagtgc
      481 ttattaagct gactcctttg ctctccagtg ccttatatgc acccatgccc tcttttacaa
      541 ttattatttt gtcagccagt ccctgcctgt acacatgttc agcctgaaga accctgtctg
      601 caattgatcc catcagtatc acaatggcat cgccgtgaat gtctccgtcg tgccttacga
      661 gccattttcc ggccctgact accagaaagg cctctgctgc aaacaatatc agaacagaca
      721 gaaaaatcgt gccggttgat ttacaggaca tactcctatg acagatattt tttccgttat
      781 tcaagcattc ccctcagcca ccgcagataa tccgaagtga tttccgatga gcagctattt
      841 atgcttttat cgatttcgcg tatcagcgtt cttttttcct tttcacttgt tcccggatcg
      901 gatgtcttgg caacaatatc aaaaagtacc tgttttgcct cgcttcttag cattggatca
      961 ctacctgaca tcataactgc catggactga atcattacag ccccgagact ggttgcagcc
     1021 tgatttgaaa catatcttat ttcccagttc ggattgctga atccctctgt tacataatcc
     1081 ggttcagcat ccagatccct gatcctgatt ttcctgaacc tggcaaactg gtccggtttt
     1141 tcagcatggt taccctgtaa gccgatatat cctgtcaacg gtttgccggc caggctcttt
     1201 acagtgtcaa tatccgccat tgtggtgatc tggtcgttta ctgatacagt gaccatgttc
     1261 cggtcacatt tgatttccat tctgtaccat ttcccggttg tttcgtcggg ccggttcgta
     1321 acggctacat gtccgtaaag ggagccggta caatgaagat catccctcca cggcgtccag
     1381 ggcgcaagaa gctgaacttc gaaaccgctt ccgggtttcg agggatcacg ccttataaac
     1441 actccgctgt taccagcttc cgataacata tattcaagct gaagaataaa attggaatag
     1501 gccttttcag tatagatatc ggttccggat ccggtactcc tggtgatcat ttctccgtca
     1561 attacttcaa aggcgccttg tttctgaagc gtccagttgg taaggtcctt gccgttgaac
     1621 aggctgacaa aatcctgttg ttttgattca catgagaata aaaatatgga aatgacaagg
     1681 gttagcagta tactattttt cattttctta taattcagat attttacatt atgaactaag
     1741 tttccatggt tttctgtatt cccttgaaag aagataactg gctgccctgt cgccgacaaa
     1801 atcttccttt tcactgtccc atacaagttt tctcttcagc ctgaaagata tcagtgccag
     1861 gtgcatcggt attgtcattt cccgaacata gtgaagatta ctttcaggtt cctttcctga
     1921 tttaattgca tcaataaaat tctgctgatg accgggggag cggggcagtg ttaccggaac
     1981 atccggcagg tcagtcagga tctcattatt gattttgata aaacgggaag tgtaatcgca
     2041 ggtcagggtt ccgccggttc cttcgaaatg ggcaccaatg tgcatttttt ccgcaccagg
     2101 tgcttccggc ggaatggttg tccagaacac atccaggtct ttgaacttga agtcaacatc
     2161 tatccacctt ggtgcatcgg ctatcccgtc aaatggtctt tcgccccggc tgtctatact
     2221 gtacagtccc ttcggatgaa gcgacataaa catgatatcg gcaatatggc accagaaatc
     2281 ggcaaaaacc cctccggaat aatccaggaa atgacgatag gttccatgac atcttaccgg
     2341 agtatattcc tcatagggag ccggtcccag ccacatgtcc cagtcgaggg tttccggcgg
     2401 ttcctggggc gggggaaaac ccagtccggg ggaaccgcct gttttccata aacgtaccgt
     2461 atgtattttc cccagtctgc ctgattgaag caattcggta acacgatggt aattatctcc
     2521 tgcatgtatc tgtgtcccaa gctggaaaac tctgttatgc ttctcgagat ttttaagcat
     2581 ggcctgtcct tctgccaggg aataggtgag aggtttttct ccgtaaacat cctttcccga
     2641 ctgaaatgcc atgattgcta tcggtgcatg ccagtggtca ggggttgcac aggtgatggc
     2701 atcaatatcc ttcctgtcaa ggatgcggcg gaaatccgaa aatacctccg ccctggaatc
     2761 cggattgatt tcctgcagcc gggcacgggc ttccccgagc cttatcttgt caagatcgca
     2821 taatgcagca acttccactt caggtaaatt attaaaccag gtcatatggc ttctgccctg
     2881 actgccaagt ccgatgacgg ctactcttac ccggtcgctc ggagccacgg cccgcagccc
     2941 tgaagacagc agcggcagac ccattaatcc gagggtactc ctggtaatga attttcgtct
     3001 gttgattttt gaattgcttc ccataaaatg ttaattattg gttcttcatg ttaatccgtg
     3061 gattcaaaaa tatatatttg ctgtaccaga tttaagctga atattattat gatacggatg
     3121 gctgtaattt acacaaatct tttcttactt gtcatcttat tgtcctgtaa aaacaacact
     3181 gagccggata acagcaagcc ggacaggaat acttttatga atcctgttta tccttccagc
     3241 atgccagatc cgagtattat cagggctgat gacggatatt tttatctgta tgcaacagaa
     3301 gatgtgagaa atacaccgat actgcgttca tctgaccttg tcacctggaa tctgatcggt
     3361 acggccttta catcagaaac aagaccttcc tttgaaccgc ggggaggcat atgggcaccc
     3421 gatattaatt atatagccgg gaaatatgtt ctttattact caatgtcggt ttggggcggg
     3481 gaatggacct gcggaatagg agtggccgtc agtgacagcc cggaaggccc ttttacggac
     3541 aggggaaaat tattccgcag caacgaaatt gatgttcaga attccattga tcagtatttt
     3601 tttgaagaga acgggaaaaa atatcttttc tgggggagtt tccatggtat atatgctgtg
     3661 gaacttacag acgacggact ggcggtaagg gaaggcacgg aaaagagatt tattgccgga
     3721 actgcctatg aaggagcctg tatatacaaa aaggatggct tctattatct gtttgcatca
     3781 accggaacat gctgtgaggg tattaaaagc acgtacacca cggttgtggg ccggtcggaa
     3841 aatcttttcg gaccctatat ggataagtcg ggaaacagta tgagcaacaa tcatcatgaa
     3901 gttctgatca gtggtaatga tcgctttgcc ggaaccggcc ataattcgga gataatcact
     3961 gacaagaaaa ataaatcctg gattttatac catgctgttg ataaaataaa tccaagggga
     4021 agggtgctga tgctggatga agttcagtgg aaggacggat ggccttttgt tgagggcaat
     4081 acggcaagcc ttaaatggac caggccatat ttctgataca ggaccggaac agaaaggtta
     4141 tgtccccgga attccgaaat attacggcta tcatacttgc aggcgggaaa aactccagaa
     4201 tgggcgggat tgacaaaggt atgctccctg ttaagaatgt tccgatgatt gcccatatcg
     4261 cgaaacagct tgaacccttt ttcggtgagg taataatagg aggcaggact gacatatatg
     4321 gttttctggg gtacagggta attcccgata tatcggaagg catggggccc ctgatgggta
     4381 tttattcatg cctgcttgca tcggatactg acttgaattt tataaccgcc tgtgatattc
     4441 ctgatatccg gattggcctt gtaagggaaa tgcttcggtt gtctgctgaa tcagatatag
     4501 ttattcccat gggtgccgga aataaatatg aacctctgca cggggtttac aggaaatccg
     4561 ttattcctgc cgctgctgga ctcttagagg ataaaagact caggacctca gatcttttcg
     4621 aacaagtgaa aacaatgttt attccttttg atggccggga atggtatcac aacatcaata
     4681 ctcctgagga ttacagcagt tacgctaaaa aagcagatga tccggcctga aaaacagtaa
     4741 actttcccgg gaaaaaacag gtattcagat ggcctttttc tgcatcaggg gaataaaaca
     4801 gcctgccagc accaggtcag agcatgtcaa attaaagtat atttgcctag tatggaacaa
     4861 agaggatctc cttccgaact gggaacagga aacatcagaa agcttctcct gaaatatgcc
     4921 attccgtctg taattgcgat gacggcatca tcgctctaca atattaccga cagtattttt
     4981 attggtcatg gagtaggtgc tctggcgatt tccggtcttg caataacatt tccgctgatg
     5041 aacctttctg cagctttcgg ttcgcttgtg ggagtgggag ctgcgactct gatgtcgctc
     5101 cggctcgggc aaaaggatta tggcactgca aacaatatac tcggaaatgt atttgtactg
     5161 aacattattc ttggcctgat atatacggtc gtagtgctgc tgaacctgga caggatactc
     5221 tattttttcg gtgccagtga tgaaactctt ccctttgctc atgatttcat ggttataatt
     5281 tcactggcca atataataac ccatctttat ttcgggctca atgccatgct cagagcaaca
     5341 gggcatccca aaaaatcaat gttcaccacc ctgttttccg ttctgatcaa tataatactt
     5401 gcaccgattt tcattttcgt gttcagatgg gggatccgcg gagcggcact ggcaactgtt
     5461 attgcccaga catcaatgct gctctggcag atacttcact tttcaaacag ccggaatttc
     5521 attcatctga aaaaaggcag ctttcgcctg aagcgcagga tagtcactga atccctttcg
     5581 ataggaatgg caccctttat gatgaattcc gttgccagta taatagtgct ggtaataaat
     5641 accagcctga tcaggtacgg cggggacctg gctgtcggag cctatgggat aataaaccgg
     5701 attgcctttc tttttatcat ggtagtcctt ggcttcaacc agggtatgca gcccattgca
     5761 gggtacaacc acggagccgg gcagttcatg agggttaaca atgttctgaa gcatacaata
     5821 gtactggcaa cttctgttat gatggtcggt tttgccatag gtgaactgtt tccccgtgct
     5881 gtcgcttccg catttacgac ggatcaggac cttattgaca ttgttgttcc gggaatgagg
     5941 attgtgatga tgtcatttcc cataatcggt tttcagatgg taacgtcaaa ttttttccag
     6001 agtatcggga tgcctgtaaa gtctattctg atgtcacttt cgagacaggt ttttttcctg
     6061 ttgccatgtc ttcttatatt cccgcgtatt ttcggactga agggaatctg gtacagcatg
     6121 ccgacagcgg atgttatatc agcagttgtc gcagctgtcc ttcttgtcgg acaataccgg
     6181 aagaacaggt taaatgaaat tactcatcag gaaatctaac tgttaattca tgaaggaaaa
     6241 ttatataatt acaatcggac gtcagcttgg aagcggagga agggaaattg gcctgaagct
     6301 tgccgaatca ctcgggattt cattttatga taaagagctg ataagactgg catcagagaa
     6361 aagcgggctc aatgaaaaag tgtttgaaag tgctgatgag aagaaacgct tcaacatatt
     6421 ctggggattg ttcggagtgc acagc
//
LOCUS       DAOSPX010000177         6418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11120_length_6418_cov_3.512808, whole genome shotgun sequence.
ACCESSION   DAOSPX010000177 DAOSPX010000000
VERSION     DAOSPX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11120_length_6418_cov_3.512808"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1780)
                     /locus_tag="PLZ75_09115"
     CDS             complement(<1..1780)
                     /locus_tag="PLZ75_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS72591.1"
                     /translation="MKSDILRMILKSLVYHRKDAVYQIIIISVLSAVISGSLFTGHSV
                     RSSLKRASLEKLGSTDIIISSGLRYFDPALSEKVSAATGNPAVSILETDGYCSNFSTG
                     VTALNVRIYGTDDTFFPFHGSGSLRIARGEAGINQSLARHLDIGEGEEIIVRFRETDP
                     LPSNAPFAPAKDDHGSVVLKVSHIIPPERGGNFSPGISQQIPMVLFLNMADMAGGQNK
                     RIQANRILVSQVNKADYNEILSSVLTPADIGLSLRTSAKTGERELISDRIFLDSLLVS
                     EILGNIPTGEPVLTYLVNSFRIRDKSTPYSFVAALPGHLYPGIDTGGIIINKWLAADL
                     DAVPGDTVRLSWYDPASGKSLEEKSRDFYIAAIAGDNGHYADSSLMPDFPGISGSTTC
                     AGWDAGVPILLAQIRKKDEDYWNRYRGTPKAFISYEIGKRLWGNNFGTATALRFPSSL
                     SIEEIDGRLRSSLIPSVTGFSISNARDSALEGAAQSVDFSSLFLSLSFFMIVSCLILL
                     SFAVSVYFDTRKKQTAIMYALGFRNSFLRNQLFCESGLLSFAGALPGVFLGYLLNLLI
                     IRALNSVWSGAVQTTALSADFSILPLL"
     gene            complement(1825..2493)
                     /locus_tag="PLZ75_09120"
     CDS             complement(1825..2493)
                     /locus_tag="PLZ75_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS72592.1"
                     /translation="MLELKKVSKSYTQRGMVLGDLDLVVRDGDAIAVTGPSGSGKTTL
                     INIIGTLDHPDSGDVIFRGRSVSTLDADESASYRNRNIGFVFQDHLLLPHLTVGENIL
                     LPLFASDIGLSEYEEKKKNASGLMKRIGISPLAGKYPFQISGGEAQRATLVRALINKP
                     SLLLADEPTGSLDAENARILGDLLVEINRETGLTLLIVTHSDALASRMKTHLILENGK
                     LKTI"
     gene            complement(2506..3786)
                     /locus_tag="PLZ75_09125"
     CDS             complement(2506..3786)
                     /locus_tag="PLZ75_09125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS72593.1"
                     /translation="MKVIYLITGSGGSFYCANCYRDMLYFRAIRQVPGITANAIPLYL
                     PPEDDSSVTGFDRHVFFGAISMFLREKAGFLKNMPAFLERFFDMTPFLKLASRQAGTT
                     SVEGYEELTINMIEGDNAFRKNEVDRLVKYLMKGGKPDVIHLSNALILGLARQLKNRM
                     KVKVVCSLLNEDDWIDDMAEPWQSRAWEMIGRESVYVDHFITPSNYYRQLFISKTGVK
                     GDNIHVIPLGFDPEPPYGERNTNRPPALGYFSRISQHNGFDKLTDAFIELKTKYSMHD
                     LGFHVCGGYTGIDKAFISDQIRKIRDHGFQKSIRIYPEFQGNKKMEFFNNVDVISVPV
                     RKYDGYGLYILEANSSGIPVVQPATGAFPEIIATTGGGITYQPDTVEELSSSLHRLLT
                     DRELAAKLGQTGKERVLTKLSIEKMSAGMYEVYK"
     gene            complement(3922..5037)
                     /locus_tag="PLZ75_09130"
     CDS             complement(3922..5037)
                     /locus_tag="PLZ75_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS72594.1"
                     /translation="MNQLFLLIFFSALSLVSLSQKPGADWPVFRGTYNLTGYAASALP
                     AAPALLWSVSTGEGSKSSPVISDGIIYFGNLKGNVYAVSPEGKIIWKKETGGTAESPP
                     MIYGDYLFIGGGDGIMRAFDKKTGRLLWSYSTDNQIVGSANIWSAGKSTGLIFGSYDY
                     YLHCVDPLTGRLLWKLETENYVNGTPAVMNNRIVFGGCDGMVRIADPLTGRERNRFET
                     GGYIAASPAISGERAWFGDYDGNLYCLDMISGKMVWEVPAGKGSGAIMAIPAAGRNML
                     VVGNEDKYLYCYNSGSGELIWKFRTNGRITGSAVITPSGVLFGSMDGNVYILGLQDGG
                     KKWSFNAGAPVSSSPAVTTGRFYFLTEDGRLLAFGTK"
     gene            complement(5122..6177)
                     /locus_tag="PLZ75_09135"
     CDS             complement(5122..6177)
                     /locus_tag="PLZ75_09135"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOS72595.1"
                     /translation="MFKIRINNIEVDAEAGETILSVSQRAGVSIPTLCHMDGIEHYTS
                     CMVCMVRDKASDSYLPSCTALVREGMDIDASGEDVVSARKKAVELLLSEHRAECEAPC
                     RIVCPAGYDIPRLNRLLAAGEYIKAIELVMSELDAPEIRCLSCRGYCENACRRRKIDR
                     QVSIRNIRIFIYHNLNYQGGPQKPVELTEFPDFKKIFSSRTGRIEEEELKEWLKECRE
                     AGKRFESVTDFISAGEEAKNCMHCDCRASDNCRLRELAQELGIRDRGNKVAGLPLTKK
                     ISQGTGLIFEHAKCIKCGLCVRICEDKKEEPSLCFINRGFVSLISEPLTAEFDDIPAA
                     QAELCVNVCPTGALSFF"
     gene            complement(6170..>6418)
                     /locus_tag="PLZ75_09140"
     CDS             complement(6170..>6418)
                     /locus_tag="PLZ75_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS72596.1"
                     /translation="FRKEYEAHASGICPAKKCRELIRYVITDKCTGCTKCSQECPVGA
                     IGFRPYEKHEINQELCTKCDNCRIVCPENAVEIMDNNV"
BASE COUNT         1540 a         1623 c         1451 g         1804 t
ORIGIN      
        1 aaaggagagg caggatgctg aaatcagctg acagggcagt ggtttgcaca gctccggacc
       61 atactgagtt gagtgccctt atgatcagga ggttcagcag atatccgagg aacactccgg
      121 gcaatgcccc ggcaaatgac aataagcccg attcgcagaa aagctgattt cttaaaaaac
      181 tgttcctgaa tcccagtgca tacatgattg ctgtctgctt cttccttgtg tcgaaataca
      241 ccgaaacagc aaacgataaa agtatcaggc atgatacaat catgaagaag ctgaggctga
      301 ggaaaagtga gctgaaatcc acgctttgtg cagcaccttc cagggctgag tccctggcat
      361 ttgaaatgct gaagcccgta acggacggaa tgagggaaga cctgagcctt ccgtctattt
      421 cctcaatgga taacgatgag ggaaacctta atgcagtggc tgttccgaaa ttatttcccc
      481 acagtctttt cccgatttca tagctgatga atgctttcgg cgtccccctg tatctgttcc
      541 agtaatcctc atccttcttc cttatctgag cgagcaggat gggaaccccg gcatcccagc
      601 ccgcacaggt ggtactgccc gatattcccg gaaaatcggg cataagagag gagtctgcat
      661 agtggccatt atccccggca atggcagcaa tgtaaaaatc cctgcttttc tcctccaggc
      721 ttttccctga agcaggatca taccagctta atcttaccgt atctcccgga acagcatcaa
      781 gatcggcggc aagccatttg ttgataatga tgcctccggt atcaatgccc gggtacaaat
      841 gtccgggaag ggcagcgaca aatgaatatg gagttgattt gtccctgatc cggaaactgt
      901 tgacaagata agtcagaacc ggctctcccg ttggtatgtt tccaagtatt tccgaaacca
      961 gaaggctgtc gagaaatatc cggtcggaga tgagttccct ttctccagtt ttggcagaag
     1021 ttcttaagga taatccgata tcagccggtg taagcacgct tgataatatt tcattataat
     1081 cagccttgtt cacctgactg acaagaatcc tgttggcctg tatccttttg ttctgtcccc
     1141 cggccatgtc ggccatgttc aggaaaagga ccatgggtat ctgctggctg atccccggtg
     1201 aaaaattgcc tcccctttcc ggaggaatta tatgacttac tttcagcact acagaaccat
     1261 gatcatcctt tgccggggca aaaggggcat ttgacgggag gggatcggtt tccctgaagc
     1321 gtacaatgat ttcttcacct tcgccaatgt ccagatgccg tgcaaggctc tggttaatac
     1381 cagcctcacc cctggctatc cggagtgaac ctgatccgtg gaagggaaag aaagtgtcat
     1441 ctgttccata gatccttacg ttcagtgccg ttactcctgt tgagaaatta ctgcaatatc
     1501 cgtctgtttc cagaattgaa acagccgggt ttcctgtagc tgccgaaact ttttctgaaa
     1561 gggcggggtc aaaatatctc agccctgaac tgattataat atcggtgctg ccaagttttt
     1621 ccagtgatgc ccgcttcagg ctgctgcgca cggaatgtcc cgtgaaaagg gaaccgctga
     1681 ttacagctga cagaactgat atgataatga tttgataaac agcatccttc ctgtgatata
     1741 ccagggattt taatatcatt ctgaggatgt ctgacttcat tggtatgatg tttttcagtt
     1801 tactgttcaa aggatgatct tcatttatat tgttttcagt ttgccgtttt caaggatcag
     1861 gtgtgttttc attcttgagg caagtgcatc tgaatgggtt acaatgagca gtgtgaggcc
     1921 cgtttcccgg ttgatctcca caagcagatc gccaagtatt ctggcatttt ccgcgtcgag
     1981 agatccggtc ggctcatcag cgagaaggag ggagggtttg tttatcagtg cccggaccag
     2041 ggtagccctc tgtgcctcgc ctcccgatat ctgaaaggga tacttgccgg caaggggtga
     2101 gatgccgatc ctcttcatca gtccggatgc atttttcttt ttttcttcat attctgaaag
     2161 tccgatatct gaggcaaaga gcggaagaag tatgttttcc ccgactgtaa gatggggaag
     2221 aagaagatgg tcctggaaaa cgaatccgat attcctgttc ctgtaggaag ctgattcatc
     2281 tgcatccagt gtacttactg acctgccccg gaagatcaca tcccccgaat cgggatgatc
     2341 aagtgtgccg ataatgttaa tcagggtggt ctttccggag ccggacggcc ctgtcacggc
     2401 aatggcatca ccatccctga cgacaagatc cagatccccg agaaccattc cccgttgtgt
     2461 gtaggacttt gatacttttt taagctccag catactgttc tgaatttatt tatacacttc
     2521 atacatgccg gccgacattt tctcaatcga aagttttgtc agaacccgtt ccttcccggt
     2581 ctgccccagt tttgcagcca gctccctgtc cgttaagagc ctgtgcagtg atgaagacag
     2641 ttcctcaacg gtatcaggct gataagtaat tcctccaccg gttgtggcaa ttatttcggg
     2701 gaatgcgccc gtggcaggtt gtaccacagg tattccgctg ctgttggctt ccagtatata
     2761 caggccgtat ccgtcatatt tccgtacagg aacactgatc acgtcaacat tgttaaaaaa
     2821 ttccattttt ttgttaccct ggaattcagg atagattctt atgcttttct gaaagccatg
     2881 atccctgatt tttctgatct ggtctgaaat aaaagccttg tcaataccgg tatagcctcc
     2941 gcagacatga aaaccgaggt catgcattga atattttgtt ttcagttcaa tgaatgcgtc
     3001 tgtgagttta tcaaatccgt tgtgttgact gatgcggctg aaataaccca gggcaggagg
     3061 gcggtttgtg tttctttccc cgtaaggcgg ctccggatcg aagcccaggg ggattacatg
     3121 aatattgtct cctttcacac cggtctttga aatgaacaac tgcctgtaat agttgctggg
     3181 tgtgatgaaa tgatccacgt acaccgattc cctgcctatc atttcccatg ccctgctctg
     3241 ccatggctcc gccatgtcat caatccagtc atcctcattg agcagggagc ataccacctt
     3301 taccttcatc ctgttcttga gctgcctggc caggcccagg ataagcgcgt tggagagatg
     3361 aatcacatcc ggctttcctc ccttcatcag gtattttacc agcctgtcaa cctcattttt
     3421 gcggaaggca ttgtcgcctt ctatcatgtt tattgtaagt tcctcatatc cttccacgct
     3481 ggttgttccc gcctgccggg aggcaagttt cagaaagggt gtcatgtcga aaaacctttc
     3541 cagaaaggcc ggcatatttt tgaggaagcc ggctttttcc ctgaggaaca tcgagatggc
     3601 accaaaaaag acatgcctgt caaatcctgt taccgagctg tcatcttcag gcgggagata
     3661 cagtggtatg gcatttgcgg ttattccggg aacctggcgg attgccctga aataaagcat
     3721 gtcgcggtaa cagtttgcac agtagaatga tcctcccgat cccgttatca gataaataac
     3781 cttcatgctt acatcctttc ttccatatat tttaatactc tttcagatcc ggtacaggtt
     3841 cgaagttact gcctcctgcg aaagcccttt accggcaatc ttcactttgt ggcgaaggca
     3901 gccggaagcc aatgctgttc cttactttgt tccgaaagcc agtaaacgtc cgtcttcggt
     3961 aaggaaataa aatcttcctg ttgtgacagc cggagagctg ctgaccggcg ctccggcatt
     4021 gaagctccat tttttcccgc catcctgcag tccaaggata taaacatttc cgtccatgct
     4081 tccgaaaagc actcccgatg gcgttatcac tgccgaaccg gtgatccggc cgtttgtcct
     4141 gaatttccag ataagctcac ctgacccgga gttgtaacag taaaggtatt tatcctcatt
     4201 tcccacaaca agcatattcc tgccggcagc cggaatagcc attattgcgc cggatccttt
     4261 ccctgcaggc acttcccata ccattttgcc gcttatcata tccaggcagt aaaggtttcc
     4321 gtcataatcg ccgaaccagg ccctttcgcc ggatattgcc ggagacgcgg ctatatatcc
     4381 gccggtttcg aacctgtttc tttcccttcc ggtgagagga tccgcaattc tgaccatacc
     4441 gtcgcaaccc ccgaaaacta tcctgttatt catcacagcc ggggtgccgt tgacataatt
     4501 ttccgtttca agtttccaca gtaagcggcc ggtgagcgga tcaacacaat gaagatagta
     4561 gtcataactg ccgaaaatca gtccggttga ttttccggca ctccagatat ttgccgatcc
     4621 cactatctgg ttatctgttg aatatgacca taacagcctg cccgtttttt tgtcaaaggc
     4681 acgcattatc ccgtcaccgc cgccgatgaa aagataatcg ccgtaaatca ttggcggtga
     4741 ttcagccgtt ccccctgttt cctttttcca tatgattttg ccttccgggg atacagcata
     4801 cacattgcct ttcagattgc cgaaatagat tattccgtcg ctgatcactg gagatgattt
     4861 ggaaccctca cccgttgaga cgctccagag aagtgcggga gcggccggca gggcggaggc
     4921 tgcatatccg gtaagattgt atgtgccccg gaaaacaggc cagtccgctc cgggtttttg
     4981 tgacagggaa acaagactga gcgctgagaa aaatatcaat aggaaaagtt gattcattat
     5041 ggcttaaact tgattatata acggttttat tccggcatca tgaagtctta aaatgttttc
     5101 attgcttttt cttatgtttt attaaaaaaa gctcagggct cctgtggggc agacattaac
     5161 gcacagctct gcctgtgctg ccggtatatc atcaaactca gccgtaagcg gttccgatat
     5221 aagggataca aatcccctgt ttatgaagca cagggagggt tcttcctttt tgtcctcaca
     5281 gattctcaca caaagcccgc atttaataca ttttgcatgt tcaaaaatca ggcctgttcc
     5341 ctggctgatc ttttttgtaa gcggcaaacc ggccactttg tttcccctgt cccttattcc
     5401 gagttcctgt gcaagttccc tcagacggca attgtccgat gccctgcagt cgcagtgcat
     5461 acagtttttt gcttcttcac ctgctgaaat gaagtctgtg acagattcaa accgtttccc
     5521 ggcctccctg cattccttaa gccattcctt cagttcctct tcctcaattc tcccggtccg
     5581 ggatgaaaag atttttttga aatcagggaa ttcagtcagt tctacaggtt tctgaggacc
     5641 gccctggtaa ttcagattgt gataaataaa gattctgatg ttccggatgg acacctgtct
     5701 gtcaattttt cttcttctgc atgcattttc gcaatacccc cggcagctga ggcatctaat
     5761 ctccggtgca tccagttccg acatcacaag ttctatggct ttaatatatt ctcctgcggc
     5821 aagcagcctg ttcaggcgcg gaatgtcata acctgccgga cagactatcc tgcagggtgc
     5881 ctcgcattcg gcgcggtgtt ccgaaagcag aagctccacc gcctttttcc tggctgacac
     5941 aacatcctct ccggaagcat ctatgtccat cccttcccgt acaagtgccg tgcatgaggg
     6001 aaggtagctg tccgaagcct tatccctgac catgcatacc atgcatgagg tataatgttc
     6061 aataccgtcc atgtggcaca gtgtgggaat ggaaacaccg gccctctggc tgactgacag
     6121 aatggtttca ccggcttcgg catccacttc aatattgttt attctgatct taaacattat
     6181 tatccattat ttcgacagca ttttcaggac aaacaattct gcaattatca catttagtac
     6241 acaattcctg gtttatttca tgtttttcgt agggtctgaa acctatcgcg cctaccgggc
     6301 attcctgaga gcattttgta cagcccgtgc acttatcggt aataacatac ctgatcagtt
     6361 ccctgcattt tttcgcagga cagatacctg aagcatgagc ctcatattcc ttacggaa
//
LOCUS       DAOSPX010000178         6390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11173_length_6390_cov_4.484294, whole genome shotgun sequence.
ACCESSION   DAOSPX010000178 DAOSPX010000000
VERSION     DAOSPX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11173_length_6390_cov_4.484294"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3837
                     /locus_tag="PLZ75_09145"
     CDS             <1..3837
                     /locus_tag="PLZ75_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOS72597.1"
                     /translation="ICELSIFAQVSGIKTIDTRGNDSGSKGIEIDIVDKSDSDVFPSM
                     YVEPDGTAHLAYVKNNTLMYAKKGRQSEWVYKSLGSCIDCNQNDIVMDKTGNIQIVVQ
                     NSVWKNTYGVHGHFHVTVTPQGTYNKRSLFYAAANWGFHSMSLKVDSHNELHLVFQGS
                     PGSAWPAPLFEMHTSGGKWTKPELIPSTIHAYDHVDMEVDSEDNLHISYYSTDIGFGY
                     MKKDAGRPWSEPEVPEPGWSGAQLEGMVTSIITDDDLNPHISYVGQVNHDNRENIKYA
                     WKKNGKWNISMVDQGEHRSAGNDIVLDKDGYPHFSYALIDKNEMRYATNKAGIWIKKV
                     FDTSPGSLWPHTIDMGIDQDNHVHIAYPKESDPYKVAYALISPLGYFDVEPDILDFRE
                     VLPGATKTLTMQIINQTSQSLEIDSITLRDPRLSKDKNSFIINSNATGTVNITLNHTN
                     RSWRDNLLSIWYGGLCADVPVQATSWQPVLTVDPSSVRFEDTPLNTTTTTKVKLTNTG
                     NLDLIFSKIEEYKVLGSLEPDYFSLVGHNCSILRPGQFCEVELSFKPGKTGNHYSYLH
                     IYSNDPVNPYKKISLSGTTAYPLISCDQSKINFGYCTVGQSVTKQLTVKNVGRLPLNI
                     TRATISGSSANQFTLENPCTILQPDESCVMQITMTPTSAADLTATLTIVSNSQYFSTY
                     NVSLTGSTYLKNLEITPALIDFGEINTGAIFSRQIQLRNTGSGNINISGIELSGTDMY
                     EFMPEHNCTIINEGATCNVTVWFFPLFPGSKTASLVVVSDDAYEPTQSVILQGTGGSA
                     LPLEATINADPGIGTAPMEVQFSAKITGGQPPFRYQWDFGDLTVSEEKAPVHTYDEPN
                     LYNVSLQVEDVHGSKTSAGKPVFVRTGDDLIVRASATRVNGPVPLEVQLDAIITGGQQ
                     PFTYLWEFRDGTTSNIMNPVHIYNSPGSYWARITVTDTDADVGRDSVLITVRSPNSIA
                     GQIWDETGTRPVIKSVTLLYPESDITHPLNLSLNGSNSYMFPNLSEANYTVRVLPDAV
                     LYPGYLPTYLGNTLSMFEATWIQASGSITDKDIRLINIPPPNTGSYLISGQMIQTFDG
                     KGLTVTFNKADLKGDPVAGALVYLKDSADGSLKAYDITAGDGSFEFGNLPAGSYYFIA
                     DWKGKPMDAGNIPLTVDASRNTVEILATVGTDNIKITDTPAGSEDVNLSGIKIYPVPA
                     GDHLVIEIPEGLFLGNSIRLSIFDLSGKCVLVNDHFNLSSGPVTINIAGIPEGVYLMN
                     LSDGKIVHRLKIIRMK"
     gene            complement(4033..5163)
                     /locus_tag="PLZ75_09150"
     CDS             complement(4033..5163)
                     /locus_tag="PLZ75_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS72598.1"
                     /translation="MGTEKNNPFRYGIAVDDPYFIDREQELKDIPKWLKNGQSLVIYS
                     PRRYGKTSLILKILNKLKDEGFKTIYIDFFKVTSRRRFTELYFNEIIKQLTSWEKALR
                     KISLLTKRIRPVVTLDNQGLPNISVRYEDGSDDVDMTEVFDLPQKLAEKKQWIVVFDE
                     FQDVEKLNGESFEKELRSALIHHTKVSYVFMGSKMHMLLNMFTHKNRAFYQFGKLLEL
                     KKIPKNQLIDFMEAGFNNSGIKYIVGVAGNIANICNDIPHYVQYLAAGAWEEALENNL
                     ILDQSALGRAVDKIITNQTDYFMQQLEELTAHQQRVLLAVSVENKNLFTASFAERFHL
                     SPVSSVQRSLQKLLKKGILTKHERTYFFTDPFFKLWIERELS"
     gene            5240..5620
                     /locus_tag="PLZ75_09155"
     CDS             5240..5620
                     /locus_tag="PLZ75_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOS72599.1"
                     /translation="MLKKSSCFYLISIVKGNYFEHYQICGNNDLLTGINRFPCTYCLV
                     FYNSLIRKKNDVENAFASIDVMLKRRHDLIPAPVDTVKGYVNYERDLLTEITELRVLF
                     SGRRNNQGCDSPISGKDSPVFSWH"
     gene            complement(6142..6215)
                     /locus_tag="PLZ75_09160"
     tRNA            complement(6142..6215)
                     /locus_tag="PLZ75_09160"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6179..6181),aa:Ala,seq:tgc)
BASE COUNT         1929 a         1345 c         1279 g         1837 t
ORIGIN      
        1 atttgtgaat tatcaatatt cgcacaggta tccggcatca aaaccataga tacacggggg
       61 aatgattccg gttccaaggg cattgaaata gacatagttg ataaaagtga ttcggatgta
      121 ttcccttcaa tgtacgttga gcctgacggt actgcccacc tggcatatgt taaaaataac
      181 acactgatgt atgcaaaaaa aggccgtcag agtgagtggg tctacaaatc acttggcagc
      241 tgtatagact gcaatcagaa cgatattgtt atggataaaa ccggcaatat tcagattgtt
      301 gtccagaact ccgtgtggaa aaatacctac ggagtccacg gacattttca tgttactgtg
      361 accccgcagg gaacatacaa taaaagatcg ctcttttatg ccgccgctaa ttggggattt
      421 cacagtatgt ccctgaaggt cgacagccat aacgaactgc atcttgtatt tcagggaagt
      481 ccgggtagcg cctggccagc tcccttgttt gaaatgcaca cctcaggagg aaagtggaca
      541 aaacccgaat taattccttc aactatccat gcctatgatc acgttgatat ggaggttgat
      601 tcagaagata atcttcatat cagttattat tccaccgata tcggattcgg ctatatgaaa
      661 aaagacgccg gcagaccatg gagtgaaccg gaagttcctg aaccaggatg gtccggagcc
      721 cagcttgaag gaatggttac ttccataatt accgacgatg acctgaatcc tcatatttct
      781 tatgtgggac aggtaaacca cgacaacagg gaaaatatta aatatgcctg gaagaagaat
      841 gggaaatgga atatcagcat ggttgaccag ggcgaacatc gaagtgcagg taatgatatc
      901 gttcttgata aggatggata tcctcatttc tcttatgctc tgattgataa aaacgaaatg
      961 agatatgcaa caaacaaggc aggaatctgg atcaagaagg tatttgacac gagtccggga
     1021 agtctgtggc cgcatactat tgatatgggt attgatcagg ataatcatgt tcatatcgca
     1081 tatcctaaag aatctgatcc atacaaggtt gcttatgctt taatatcacc tcttggttat
     1141 ttcgatgttg aacctgatat acttgacttc agggaagtgc tgcctggcgc tacgaaaaca
     1201 ctgacaatgc agataattaa tcagacatct cagagcttgg aaatagattc tataaccctc
     1261 agagatccaa ggctgagcaa ggacaaaaat tcattcatta tcaatagtaa tgccactggc
     1321 acagtaaata tcaccttaaa tcatacaaac aggtcctgga gagacaacct gttaagcatt
     1381 tggtatggcg ggttatgtgc agatgttccc gtgcaggcaa ccagctggca gccagtgctg
     1441 acagtcgatc cttcttctgt cagatttgaa gatacacctc taaacacaac cacaacaaca
     1501 aaagtaaaac tcaccaacac cggtaatctt gatctgatct tctcaaaaat tgaagagtat
     1561 aaagtcctgg gaagcctgga acctgactat ttcagtctgg tgggccataa ttgttctata
     1621 ctccggccag gacagttctg tgaagtggag ttaagcttta aaccgggaaa gaccggcaat
     1681 cactattctt atcttcacat ttattcaaat gatcccgtaa atccttataa gaaaatctct
     1741 ctttcgggca cgacagcata tccgttgatc agttgcgatc aatcaaaaat caacttcggg
     1801 tattgcacgg ttggtcagag cgttacgaaa caacttactg ttaaaaatgt cggcaggctg
     1861 ccattaaaca ttaccagggc tacaatctcc ggcagcagtg caaatcagtt tacactggaa
     1921 aatccatgta caattctgca gccggatgaa tcgtgtgtaa tgcagatcac catgactccc
     1981 accagtgctg cagatttaac ggcaaccctg acaatagtat caaactccca gtatttcagc
     2041 acttataatg taagcctgac aggatcgact tatttaaaaa atctggaaat cacaccggcg
     2101 ctgatagatt tcggggagat aaacacgggt gcaatattct ccagacagat tcagctgcgc
     2161 aataccggga gcgggaatat caatatttcc ggcattgagt tgtctggcac ggacatgtat
     2221 gaattcatgc ctgaacacaa ttgcactata attaatgaag gtgcaacatg taatgttaca
     2281 gtatggtttt ttccgctttt ccccggttca aaaacagctt cacttgttgt tgtatcagat
     2341 gatgcttatg aaccaacaca gtctgtaatc cttcagggta cgggaggatc tgccttgccg
     2401 cttgaagcaa ccatcaatgc tgatcccgga atcggaacag cgccaatgga ggttcagttc
     2461 tctgcaaaaa taacaggggg acagccacct ttcagatacc agtgggattt tggtgatctg
     2521 actgtcagtg aggaaaaagc acctgtgcat acatatgatg aaccaaattt gtataatgtc
     2581 tctcttcagg tggaagatgt tcatggttcc aaaaccagtg ccggaaaacc ggttttcgtg
     2641 cgaacgggtg acgacctcat agtaagagct tctgcaacgc gggtcaacgg tccggtgccc
     2701 ctcgaggtac agttagatgc aattataacg ggaggtcagc agccttttac atatctgtgg
     2761 gaattcaggg acggcacaac cagcaacatc atgaatccgg ttcatattta taattcaccc
     2821 ggcagttact gggcaaggat cactgtcaca gacacggatg ctgatgtagg aagggattcc
     2881 gtgctgatca ctgtccggtc gcccaacagc attgcaggac agatctggga tgaaacggga
     2941 accagaccgg taataaaatc tgttaccctt ctgtatccgg aatcggatat cacccatccc
     3001 ttaaatcttt cattgaacgg tagcaactca tacatgtttc cgaacctttc tgaggcaaac
     3061 tatactgtca gggttcttcc tgatgctgtt ctatatcccg gttacctgcc gacatatctc
     3121 ggcaacaccc tttccatgtt tgaagccacc tggattcagg cttccggcag tattacagat
     3181 aaagatatca gactcattaa tatacctccg ccaaatacag gttcatatct gatctccggg
     3241 caaatgatcc agacatttga cggaaaagga ctcacagtaa catttaataa ggctgattta
     3301 aaaggtgatc ctgttgccgg agcgcttgta tatctgaaag attctgcaga tggatccctg
     3361 aaagcttatg atattaccgc cggggacgga tcatttgaat tcggcaatct tccggcaggg
     3421 tcatattatt tcattgccga ctggaaaggg aaaccgatgg atgcaggaaa tattccgcta
     3481 acagttgatg cctcaagaaa tactgtcgag attctggcaa ctgtgggtac tgacaatata
     3541 aaaattaccg atacacccgc cgggtcagag gatgtaaatc tttcaggcat aaaaatctat
     3601 ccggttcctg caggcgatca cctggtaatt gaaataccgg aaggactatt cctcggaaat
     3661 tccatcagat taagcatttt cgatctctca ggcaaatgtg ttctggtcaa tgatcatttt
     3721 aatctgtcat ccggtccggt cactattaat attgccggta tccctgaagg tgtttacctg
     3781 atgaatctga gcgacggaaa aattgttcac aggctgaaaa tcataagaat gaaataagtt
     3841 cggccaaaga acatgtttaa attataacta atcaaattat aataattcaa gactatcact
     3901 ttcatatgaa accttcatcc cgcatatgcg ggacaaaaga ttatgaaaat atttttcaat
     3961 catgaaggtt tcatgtgaat cacttaattt tcaacaatat agtaatatta ttttcacatg
     4021 aatccttgtt aatcaggata attctctctc tatccagagc ttgaaaaaag gatcggtgaa
     4081 aaaataagtt ctttcatgtt ttgtaagaat tccttttttc aataattttt gtaaggacct
     4141 ttgaacagat gagaccggag ataagtgaaa tctttcagca aacgaggccg taaacagatt
     4201 tttattttcc acgcttactg ccagtaatac cctttgctga tgtgccgtaa gttcctcaag
     4261 ctgttgcata aaataatctg tctgattcgt aattatctta tcaacagctc tgccaagagc
     4321 agactgatcc agaatcaaat tgttttccag ggcttcctcc catgcaccgg cggcaagata
     4381 ttgaacataa tgagggatat cgttacagat atttgctata tttcctgcaa cccctacgat
     4441 atattttatt ccagagttat taaatccggc ttccatgaaa tcaatcaact gattttttgg
     4501 aatttttttc aattccaata gttttccaaa ctgataaaaa gcccggtttt tgtgagtgaa
     4561 catatttaaa agcatatgca tttttgaacc catgaataca taacttacct tcgtatgatg
     4621 tattaaagct gaccttagtt ccttttcaaa gctctcacca ttaagctttt ccacatcctg
     4681 aaactcatca aaaacaacaa tccactgctt tttctccgca agtttctgtg gaagatcaaa
     4741 gacctctgtc atatcaacat catcagaacc atcttcatat ctcacgctga tatttggtaa
     4801 accctgattg tcgagtgtga ctacaggccg gatcctcttt gtaagcagag atattttgcg
     4861 taaagccttt tcccatgagg tcaactgttt gataatctca ttgaaatata attcagtaaa
     4921 gcgacgtctg gatgtaactt tgaaaaaatc aatgtaaatt gttttgaatc cttcatcttt
     4981 aagtttattc agaattttaa gtatcaaaga agtcttacca tacctacggg gagagtatat
     5041 tacaagactt tgcccattct tcaaccattt aggtatatct ttcagttcct gttctctgtc
     5101 aatgaaatat gggtcatcga cagcaattcc atatcggaaa ggattatttt tttcagttcc
     5161 cattaaaaaa tgttttaaac aaatttacgc aattttgcat tacgcaacaa tgtataattt
     5221 ctaagattcc tctattttta tgctaaaaaa atcttcctgt ttttacctta tttccatagt
     5281 aaaagggaat tactttgaac attatcaaat ctgtggaaat aatgatctac ttactggtat
     5341 taatcgtttt ccttgcactt attgccttgt tttttataat tcccttatca gaaagaaaaa
     5401 cgacgttgaa aatgcatttg catctattga tgtaatgcta aagagaaggc atgaccttat
     5461 tccggcacca gtagatacag ttaaaggtta cgtgaattat gaaagggacc tgcttacaga
     5521 aattaccgaa ctcagggttc ttttttccgg tcgcagaaat aatcagggct gtgatagccc
     5581 gatatcagga aaggattctc cggttttctc atggcattaa ttgagtatta tttacatctt
     5641 gcgatttaca aattgtaaat tacaaaatgt ataaatattt aaataatatt atcacaaagt
     5701 tgcagcaaac agttatcagg aatgcaatat ttctgataaa tttcaggatg gttcggaaga
     5761 attaagaaat taaccacatt tgtttgaagt ccggagtccg gagtccgaag aatctacctt
     5821 ccctgacttc cgtcttccat cttaggtctt ccgacttctg tctaatatta tgactaactt
     5881 ttttttgcca atatgtcaga acttttcaag ttgggagttt gaagttaatc cataactcca
     5941 aactaaattg tggagaataa gggattactc gcttcgctcg tgatcccggc gggagggctc
     6001 tgtaaactaa atcactggct tattcgcttc gctcataagg tctttttatt ttcatctgtc
     6061 ctcactgcgg caaaccggat tcggccatat gaaaataaaa aatcctgtca gctgggcagc
     6121 tcacaggagt gatttagttt gtggagaata agggattcga acccttgacc ccctgcttgc
     6181 aaagcaggtg ctctggccag ctgagctaat tccccaaata agtattccaa aaaactgcgg
     6241 attaagcccc gcttgaccct cccgaccaaa gtcgggatgc tctggccagc tgagctaatt
     6301 ccccaaatta gtattccaaa aagctgcgga ttaagcccgc ttgaccctcc cgactaaagt
     6361 cgggatgctc tggccagctg agctaattcc
//
LOCUS       DAOSPX010000179         6371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11230_length_6371_cov_5.276441, whole genome shotgun sequence.
ACCESSION   DAOSPX010000179 DAOSPX010000000
VERSION     DAOSPX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11230_length_6371_cov_5.276441"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..2889
                     /gene="leuS"
                     /locus_tag="PLZ75_09165"
     CDS             223..2889
                     /gene="leuS"
                     /locus_tag="PLZ75_09165"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOS72600.1"
                     /translation="MYYNFRDIEKKWQKYWEDNKTFKTPDDFSNPKKCYILDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMKGFNVLHPMGWDAFGLPAEQYAIQTGTHPADTTK
                     DNCDTFRRQIKELGLSYDWDREINTTDPKYFKWTQWIFIQLFNTWFDEELQKGRPVSE
                     LKVPDDIRKSGEAAVQEYVNSRRLAYYDNAMVWWCPDCRIVCSNEEVLYDGSHEKCGN
                     PVEKKNLRQWMLRIPLYAERLLSGIDGLDWPEGIKEMQRNWIGRSTGAEVDFRIDGMN
                     ESLRIFTTRPDTLFGATYMVIAPEHPLTRKVTTENHREKVENYIRAASLKSDLDRTDL
                     AKEKTGVFTGRYAVNPVNRERIPVWVADYVMMGYGTGAIMAVPAHDTRDFEFAVKFNI
                     PVICILDPDVADPGLKERIREGREVWTGDGRYINSADRNTGIDINGLNMEAGIARMIQ
                     WLESTGLGSARVNYKLRDWLFSRQRYWGEPFPVIHWEDGEISVMDEGQLPLELPELEK
                     YLPGESGESPLANAGSWLEVEDRNGRKGRRETNTMPQWAGSCWYYLRFIDPHNDTRIF
                     DPEKEKYWMPVDIYIGGAEHAVLHLLYSRFWHKVLYDLGIVSTDEPYTKLFNQGMILA
                     YAYENEAGAKIAADLVTERGGKFYNTETGAELKQIVAKMSKSLKNVVNPDDVVLKYGA
                     DSLRLYEMFMGPLEVTKPWDEKGVRGVFNFLTRVFRFFTEGENISQGNEEDPEILKKL
                     HQTIRKVETDIENLRFNTAISAMMIFLNLATKKGRVSSETAAIFAKILSPFAPHIGEE
                     LWSLLGNSESIACQPWPEVNEEYLKEEVFEYPVSFNGKLRFRIELPLSMDREEIIERV
                     VSDERAKKWLETGKLANVIVVPNRIVNVVVKN"
     gene            2901..4151
                     /locus_tag="PLZ75_09170"
     CDS             2901..4151
                     /locus_tag="PLZ75_09170"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOS72601.1"
                     /translation="MSGHYRSVIIITIILAVSSCAQSGKDEGIPYGQVTELSFTPADT
                     AAVTSPVLLCGIPADSFNVIRGKIRQNRFLSQILAEYGIGVQEIEELVRNSSDVFDVR
                     KIRAGNNYFILCDNDSAGKAKYMIYEHDPALSYIFSFNDSLNITPFSKEITRKIKYSS
                     GTVETSLWEAMVAGDLNPLLAISLSEIFAWTVDFFGLQKGDHFKVIYEELFIDDDPQG
                     IGRIFGAQFTWGGKTTNAIPFVQNGVESYYDSEGNSLRKAFLKAPLRFTRISSRFSPS
                     RLHPILRIRRPHFGVDYAAPAGTPVHAVGDGRVVSAITEGASGKMVKIVHNSVYSTAY
                     LHLSRFGPGIAAGRYVKQGDIIGYVGSTGLSTGPHLDFRFYKNGSPVDPLKVEAPPTE
                     PVLSENMESFEIARTVMESLLESI"
     gene            complement(4310..4882)
                     /locus_tag="PLZ75_09175"
     CDS             complement(4310..4882)
                     /locus_tag="PLZ75_09175"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOS72602.1"
                     /translation="MKNIAIFASGSGTNAENIIRYFSNSDSARVVLVLSNRSDAFVLK
                     RAKALSVETLFFERGDFYGSGRVPGILQARNIDFIVLAGFLWLVPEDIIERYEGRIIN
                     IHPALLPKYGGRGMYGDRVHRAVIQGCEKESGISIHYVNNNYDEGDIIFQAKCMVEPS
                     DTAETLARKIHELEYRYYPGIIEDLVKNLP"
     gene            5065..5301
                     /locus_tag="PLZ75_09180"
     CDS             5065..5301
                     /locus_tag="PLZ75_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOS72603.1"
                     /translation="MSDIATRVKEIIVDKLGVDESEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNIGIPDDQAETISTVGDAIKYIEENAK"
     gene            5326..>6371
                     /locus_tag="PLZ75_09185"
     CDS             5326..>6371
                     /locus_tag="PLZ75_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HOS72604.1"
                     /translation="MRRVVITGLGAITPLGANLHEYWEGLRQGVSGAGMITRFDTEKF
                     KTRFACEVKGYDSSKYFDRKEANKMDLYTQFAHISSDEAISDSGIDLASVNKERVGVI
                     WSSGIGGIQTFYEQIRNYVLGDGTPRFSPFFIPKMIGNIASGSISIKYGFTGLNFATV
                     SACASSTHALIDAVNYIRLGKADIIIAGGSEAAIIEPGVGGFNSMMAMSTNNAEYMTA
                     SRPFDLTRDGFVIGEGGGALIVEELEHARARGARIYAELAGTGMTADAFHMTAPHPEG
                     TGAMNVMALAIKDAGLRPDDIDYINAHGTATPLGDISEARAIIAVFGEHAFKMNISAT
                     KSMTGHLLGAAGAV"
BASE COUNT         1911 a         1419 c         1522 g         1519 t
ORIGIN      
        1 ctctcaacca acagaaccgg aatctgtcat tctatccatt gaactacggg acctgatttt
       61 ccggcacaaa aatataacca aaaagcgggt taaacaatat atcccgcaag gttattccca
      121 aagatttcat attttcgcag tccggttgaa agcggaaagc ccggagacgg aaaaaccggg
      181 ccggtttttc cacccataac aacttaaata acaaatcgca caatgtacta caacttccgt
      241 gatatagaaa agaagtggca gaaatactgg gaggacaaca aaacattcaa aacgccggat
      301 gatttttcga atccgaagaa gtgctatatt ctcgacatgt ttccttatcc ttcgggtgca
      361 ggtctccatg tcggtcatcc cgaaggttat actgcaaccg acatcataag caggtacaag
      421 agaatgaagg gattcaatgt gcttcatccc atgggctggg atgcattcgg actcccggcg
      481 gaacagtatg caatccagac aggaacgcat cctgctgaca caacaaaaga taactgcgac
      541 actttcagaa gacagataaa agaactggga ttaagctatg actgggacag ggaaataaat
      601 accactgacc cgaaatattt caaatggacc cagtggattt ttattcagct ctttaacacc
      661 tggtttgatg aagaacttca gaaaggccgc ccggtttcgg aactgaaggt tccggatgac
      721 atcagaaaat ccggtgaggc tgccgtgcag gaatatgtta actcacgcag gctggcctat
      781 tatgacaatg caatggtgtg gtggtgcccc gactgcagga tagtgtgctc caatgaggag
      841 gtactgtatg acggatccca tgaaaaatgc gggaatccgg ttgagaaaaa gaatctaagg
      901 caatggatgc tcaggatacc gctctatgct gaaagactgc taagcggaat tgacggactc
      961 gactggccgg aaggcataaa ggagatgcag agaaactgga taggcagatc aacaggtgcc
     1021 gaagtggatt tcaggatcga cggcatgaat gaaagcctca ggatttttac aacacgtccg
     1081 gataccctct tcggggccac atacatggta attgctcctg aacatcccct cacaaggaag
     1141 gttacaacag aaaaccacag ggagaaggtt gaaaactata tcagggcagc ttccctgaaa
     1201 tctgatcttg accgcaccga tctggcaaag gaaaaaaccg gggtttttac cggaagatac
     1261 gccgtcaatc ccgtgaaccg tgaaagaata ccggtatggg tagctgatta tgtcatgatg
     1321 ggatacggca ccggggcaat catggctgtt cctgcccatg acacccgcga ttttgagttt
     1381 gcggtgaaat ttaatatccc tgtaatctgc attctcgatc cggacgttgc cgaccccggc
     1441 ctgaaggaaa gaatacggga aggaagggaa gtatggaccg gggatggaag atatatcaat
     1501 tccgctgaca ggaataccgg aattgatatt aatggtctca acatggaagc cggcattgca
     1561 cggatgatac aatggcttga atccacaggc cttggaagcg ccagagtgaa ttacaagctt
     1621 cgcgactggc ttttcagccg ccagagatac tggggagagc cattccctgt catacactgg
     1681 gaagatggcg aaataagcgt tatggatgaa gggcaactgc cccttgaact gcctgaactg
     1741 gaaaagtatc ttccgggaga atcgggagaa tcacccctgg ccaatgcagg cagctggctt
     1801 gaagttgagg ataggaacgg caggaaggga aggcgtgaaa ccaataccat gccccagtgg
     1861 gcaggttcat gctggtacta cctgcggttc atagatccgc acaatgacac ccggatcttt
     1921 gatcctgaaa aagaaaaata ctggatgccg gtggatatct atatcggtgg tgcggaacat
     1981 gctgtccttc acctgctata cagccgcttc tggcataagg ttctctatga cctgggcatt
     2041 gtctcaaccg atgaacccta tacgaaactg ttcaaccagg gaatgattct ggcctatgcc
     2101 tatgaaaacg aggcaggggc caagattgct gcagacctgg tgacggaacg cggcgggaag
     2161 ttctacaata ccgaaacggg ggcagaacta aaacagattg ttgcaaaaat gtcaaaatca
     2221 ctgaagaatg tggtaaaccc cgacgatgtg gttttgaaat acggggcaga ctcactccgg
     2281 ctttatgaaa tgttcatggg acccctggaa gttacaaaac cctgggatga aaagggggtc
     2341 aggggagtct ttaattttct cacaagggtg ttccgttttt ttacagaagg tgaaaatatc
     2401 agtcagggga atgaagaaga tcctgaaatc ctgaaaaaac tgcatcagac aataagaaag
     2461 gttgaaacgg atatagaaaa cctgcgtttc aacacagcca tctcagccat gatgatcttt
     2521 ctgaacctgg caacaaagaa aggaagggta agctctgaaa cggctgccat attcgcaaaa
     2581 atactttccc cattcgcacc ccatattggt gaggaattat ggagcctgct cggaaacagt
     2641 gaatcaatag cctgtcagcc atggccggaa gtgaatgagg aataccttaa agaggaagta
     2701 tttgaatatc cggtctcatt caacggaaaa ctacgtttca ggatagaact gcccctgagc
     2761 atggacaggg aagaaataat cgaaagagtg gtttctgacg aaagggcaaa gaaatggctg
     2821 gagacaggca agctggcaaa tgtgatagtg gtaccaaacc gcatagtaaa tgtagttgta
     2881 aaaaattaaa ttactgaagg atgtcaggtc attacaggtc agtaatcatt attacaatta
     2941 tccttgcagt cagttcgtgt gcacaatcag gaaaggatga aggcattcct tacggacagg
     3001 taacggaatt atcctttacc ccggcagata cagctgcggt cacttcccct gttctgcttt
     3061 gcggaatacc tgctgattca ttcaatgtga tcaggggaaa aatccggcaa aacagattcc
     3121 tgtcacagat tctggctgaa tacggaatag gtgtacagga aatcgaggaa ctcgtgagaa
     3181 attcatcaga cgtatttgac gtgagaaaaa taagagccgg aaataattat ttcattttat
     3241 gcgataacga ttcggcagga aaggcaaaat acatgattta tgaacatgac cctgccctca
     3301 gctatatttt ctccttcaat gattccctta acatcactcc gttcagtaaa gagatcacca
     3361 ggaaaataaa atattcctca ggaactgtgg aaacttctct ctgggaagcc atggtcgccg
     3421 gagaccttaa tcccctgctg gcaatttcac tttcggagat ttttgcctgg actgtggatt
     3481 ttttcggact tcagaaagga gaccacttca aggtaattta tgaagaactg tttatagacg
     3541 atgatccgca gggtattgga agaatattcg gagctcagtt tacatggggc ggaaaaacca
     3601 ccaatgcaat accatttgtg cagaatggcg ttgaaagcta ttacgacagc gagggcaaca
     3661 gcctgagaaa agcattcctg aaggcccccc tgaggttcac gagaataagc tcgcgctttt
     3721 caccgtcgcg gctccatcca attctcagga tacgccggcc gcatttcggt gtggattatg
     3781 cagcaccggc aggaacaccc gttcatgccg taggcgacgg aagagttgtt tcagccataa
     3841 cagaaggagc ttcggggaaa atggtaaaaa tagtccacaa cagtgtctat tcaacggcat
     3901 acctgcatct cagcaggttc ggaccgggaa tagctgcagg cagatacgta aaacagggag
     3961 atataatagg atatgtcgga agcaccggac tgtcaaccgg cccgcatctg gatttcagat
     4021 tctataaaaa cggttctccg gtcgatcccc ttaaggttga agcaccacca accgaaccgg
     4081 tgctcagcga aaatatggag agtttcgaaa ttgcccggac agtaatggaa agtcttctgg
     4141 agtccatctg attcagttca ggacaatttg ctcagaacag ctttccgggg cagtttttat
     4201 gggcagttct ccagggcagt tccttcagaa atcagttccc cggcgcagtt tcccggagca
     4261 gtaacatcaa gacaattccc ccacaggata tttccccgga gaaatttcgt catggcagat
     4321 tcttcaccag atcctcaata atcccgggat aatacctgta ttccagttca tgaatcttcc
     4381 gggcaagcgt ctctgcagta tcggagggct caaccataca ttttgcctga aaaatgatgt
     4441 caccctcatc ataattgttg ttcacatagt ggatacttat tcccgattcc ttttcacaac
     4501 cctgaatcac agccctgtgt acccggtcgc catacattcc ccggcctccg tactttggaa
     4561 gcagtgcagg atgaatatta ataatacggc cctcatacct ttcaataata tcctccggga
     4621 ccagccataa aaagcctgcc agtactatga aatcaatatt cctggcctga agaatcccgg
     4681 gcacccgtcc ggatccgtaa aaatcaccgc gttcgaaaaa taatgtctcc accgaaagag
     4741 ccttagccct tttaagcaca aatgcatcgc tgcggtttga caaaacaagt accaccctcg
     4801 ccgaatcgct gttcgaaaaa tacctgatta tgttttctgc atttgtgccg ctgcccgatg
     4861 caaatattgc tatatttttc attataagat gtttacccta aattacgaaa aaaattccgg
     4921 tatccgcaac attaagtata tacaactaaa tatcaatgtt ttaagtatct gattaaataa
     4981 aaacttcaaa atcatttgaa atttaaccta caaatatatt tctttgcagg gtttacaact
     5041 taatactaac taaaaattac gattatgtct gacattgcca caagagtaaa agagattatt
     5101 gttgacaaac tcggagttga tgaatctgaa gtaactcccg aagcaagttt cacaaatgat
     5161 ctgggagcag attctcttga tacagttgaa ctgatcatgg aatttgaaaa ggaattcaat
     5221 atagggattc ccgatgatca ggcagaaacc atcagcaccg tgggagatgc aataaagtat
     5281 attgaggaaa atgcaaaata atattgcatt gaaaattttc cttttatgag aagagttgta
     5341 attaccgggc ttggagcaat aactccgctt ggggcaaatc tgcatgaata ttgggaaggc
     5401 ttaagacagg gagttagcgg agcgggcatg ataacccgct tcgatactga aaagttcaag
     5461 accagatttg cctgcgaagt caaaggatat gactcttcga aatattttga cagaaaagag
     5521 gccaataaaa tggacctcta tacacaattc gcccacattt ccagtgatga ggcgatttcg
     5581 gattcaggta ttgacctggc ttcagtcaac aaggagcgtg tcggagtaat ctggtcatcg
     5641 ggtatcggag gcattcaaac cttctatgag cagatcagaa actacgtcct cggagatggc
     5701 acaccaagat tcagtccctt ctttattccg aaaatgatag ggaatattgc atccggttcc
     5761 atttccatca aatacggctt caccggtctt aattttgcca ctgtttccgc ctgcgcatct
     5821 tctacccatg cccttatcga tgctgttaat tatatcaggc tgggaaaggc tgatataatt
     5881 atagccggag gttcagaagc agcaataatt gaacccggag tgggaggctt caactccatg
     5941 atggccatgt caacaaacaa tgccgaatac atgactgctt cccgtccctt tgatcttacg
     6001 cgcgacggat ttgtgatagg cgagggcgga ggagccctga ttgttgaaga gctggaacat
     6061 gccagggcgc ggggcgccag gatttatgcc gaacttgccg gtacgggcat gacggcagat
     6121 gcttttcaca tgactgctcc ccatcccgaa ggaacgggtg ccatgaatgt gatggcactg
     6181 gcaatcaagg atgccggcct cagaccggat gacatagatt acataaacgc tcacggcact
     6241 gcaaccccgc tgggagatat ttccgaggca agggcaatca tcgcagtttt cggtgaacat
     6301 gcctttaaaa tgaacatcag tgccaccaag tcaatgacgg gtcatcttct cggggcagcc
     6361 ggtgcagttg a
//
LOCUS       DAOSPX010000180         6370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11236_length_6370_cov_4.609184, whole genome shotgun sequence.
ACCESSION   DAOSPX010000180 DAOSPX010000000
VERSION     DAOSPX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11236_length_6370_cov_4.609184"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /gene="tatA"
                     /locus_tag="PLZ75_09190"
     CDS             complement(<1..180)
                     /gene="tatA"
                     /locus_tag="PLZ75_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOS72605.1"
                     /translation="MSGPEIFVIIIVILVLFGADKLPEMARSFGKGMREFRKATDDIR
                     REIETSTSEIRREFTE"
     gene            complement(245..3223)
                     /gene="secDF"
                     /locus_tag="PLZ75_09195"
     CDS             complement(245..3223)
                     /gene="secDF"
                     /locus_tag="PLZ75_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOS72606.1"
                     /translation="MQNKTAIMVLAIALALVSVYQLSFTGATYKVKQDAKTYAQGDLV
                     KQTYYIDSIASLPKSKWSFLGYSFKDCQKREINLGLDLKGGMNVILEVSVEDILKALS
                     NHSTDKTFLSALARAKELEKESQADFLVLFGRAFQEIDPNAKLAAIFSTVDLRDKINF
                     NSTNEQVLRVLDEEINSATKNAFNILRTRIDRFGVVQPNITQLATKGRILIELPGQTD
                     PQRVRELLQGTANLEFWETYENSEIINYLISANELLKGIEANARQAVEAEKQGAAAEA
                     ASESSGSEIQDLLDLIEKDTVQTAAASTREEFALQNPLFGILTPRVTEQGEALPSCMI
                     GLALGVDTAKVNRYLAMNQVRALFPRDLRLYWSQNPHKYDPSGSLYELHAIKVTTRDG
                     RAPLDGSVITSARPTSGMTGSEVKVDFNMNSEGAKTWARMTRENVNRCIAVVLDGYVR
                     SYPRVVGEIPTGSTEITGDFTIEEADDLANILKSGKLPAPARIVSDTVVGPTLGREAI
                     NAGLISFIIAFVIVLAYMAFYYSRSAGGVADIALFSNIFLIMGVSASLGAVFTLPGIA
                     GIVLTMGMSVDANVLIFERIREELRAGKGVKLAIADGYKGSLSAILDSNITTLLTGIV
                     LYIFGTGPIKGFATMLVIGIFTSLFSAIYISRLIYEGLLKRNARLTFSIKWTENFLRG
                     VSFDFIGKRKLFYTISSSIIIISVISLFVRGLNPGIDFTGGRTYVVRFNEAVKTADVA
                     AALTDVFGGDAPQVVTFGNESQVRITTKYRIDESGADEAVETALYEGLREMTGENLSK
                     EEFLTKYRQSSETVGPAIASDIKRRAAYAVGISLVIMFLYIFVRFKNWQYGLGAVVSL
                     AHDVIIVIGIYSLFWGIMPFSMEIDQSFIAAILTVIGYSVNDSVIIFDRIREFLPLYR
                     KRATRDVLNLAVNDTLSRTVNTGVTTILVLIMMFFFGGTTIRGFLFAMIIGVIVGTYS
                     SIFIGTTLVYDTSSKAGLKM"
     gene            complement(3358..4869)
                     /locus_tag="PLZ75_09200"
     CDS             complement(3358..4869)
                     /locus_tag="PLZ75_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS72607.1"
                     /translation="MNKTLVMNRLIIPVVVFSFLLSIKSVTAQEIRKLTLSEVIKLAE
                     DQSPLALMAKHRFRASYWQYRTYVAQYRPSLTLTGTTPDYSTAYSRVWNSQAGEWYYV
                     STNILQTIGSLSLLQNIGITGGTIALQSDLTLETDLDKAKDQTRNRYITAPLSVRFVQ
                     PLFRYNSLGWQKKTEPLKYEAAKKTFLSNIESVHMEAVRQFFSLTLAQINKQIAETNY
                     LNADTLYRMAQGRFNLGTIAEDELLQMQLSWLNSETARKEADMNLKDRELRLRSFLGF
                     NENIRLELILPSSVPDLQVDVQEVLDLAHNNNPQIINQQLNVLTAQSNLAQAKADKGL
                     NANLIASFGLRDQDPVFRYAYQESNRQQTIRLGITLPILDWGLGRGRYLMAKSSLELA
                     QVQSRQDQVDFDQNLILDVEQFNLQAEQVRIAAKSDTVAARMYEVTKARFLIGKIQVL
                     ELNNADTRKDTNRRAYVQALQNYWSYFYNLRNLALYDFLNRRPLETNYEKLLD"
     gene            complement(4862..>5119)
                     /locus_tag="PLZ75_09205"
     CDS             complement(4862..>5119)
                     /locus_tag="PLZ75_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOS72608.1"
                     /translation="AKKMDIVVQFLSEAVLISVSGGFVGIFLGVIMAKIIEQVAGIMT
                     IVSFFSVFIAFGVSAAVGVVFGYSPAKRASEKDPIESLRYE"
     assembly_gap    5120..5129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5130..6258)
                     /locus_tag="PLZ75_09210"
     CDS             complement(<5130..6258)
                     /locus_tag="PLZ75_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS72609.1"
                     /translation="MFKFIFRYFHDIEIAVESIISNKLKSMLTALGIIFGVAAVIAML
                     AIGKGAKQEIMEQMKMVGVNNILINPIVQDQSSGGEEGEKQAKKFSRGLSMLDVEAIN
                     ETLPSVKRISPEISFNSTAVMNGVKYTAKLVGVSNDYFHLYNLPLVSGAFFNSYQEEN
                     GIQVCIIGANIRAKFFSKVDPIGQYIKFNGIWLKVIGVLQKTTVSLTGFEEKGVNVYN
                     DNIYIPIQTMLLRYQNRALVNTKLASEASSNVFVMGGFGGGMGRVLSGSSDAGSDLET
                     NYNQLDRIVVQVNETEQITPSTEILSRMMIRRHSNVKDFEITVPELLLKQQQRTKDIF
                     NIVLGAIASISLVVGGIGIMNIMFASVMERIKEIGTRMAIGA"
BASE COUNT         1568 a         1572 c         1411 g         1809 t
ORIGIN      
        1 ttcggtaaat tctctccgga tttcacttgt gcttgtttca atctcgcgcc ggatatcatc
       61 cgttgccttc ctgaactctc gcattccctt tccgaagctt cttgccatct cgggaagctt
      121 gtcggctcca aacaaaacca gtattacaat aattataaca aaaatctccg gtccgctcat
      181 ttcattccct gttataattc cattccggta aagttacaaa agattaagca gaatcatatc
      241 cattttacat ctttaatccg gcctttgaag aggtatcata cacaagcgtt gttccaataa
      301 agattgagga atatgttccg actatgacac caattatcat tgcgaaaagg aatcctctga
      361 tggtagttcc tccgaagaag aacatcatta tgagaaccag tattgtcgtg actcccgtat
      421 ttactgtccg gctcagtgtg tcattcactg caaggttgag aacatcccgt gttgcacgtt
      481 tccggtagag gggaagaaat tcccttatcc tgtcaaatat tatcaccgaa tcattcaccg
      541 aataacctat aaccgtcagt atcgccgcaa taaaggactg gtcaatttcc atgctgaagg
      601 gcatgatgcc ccagaacagg gaatagattc cgataacaat gatcacgtca tgagcaaggg
      661 atacaacagc cccgagtccg tactgccagt tcttgaacct gacaaaaatg tacaggaaca
      721 ttattacaag tgatattccc acggcatatg ccgctctcct ttttatgtcg ctcgcgattg
      781 caggcccgac tgtttcagaa ctttgcctgt attttgtcag gaattcctct tttgaaagat
      841 tctctcctgt catttccctc aagccttcat agagagccgt ttcaactgct tcatccgctc
      901 cggattcatc aatacggtat tttgttgtaa tccttacctg gctttcattg ccaaaggtaa
      961 ccacctgggg agcatccccc ccgaatacat cagtaagggc tgcggcaaca tccgctgttt
     1021 tcacggcttc attgaacctg accacataag tccgtcctcc ggtaaagtca attccgggat
     1081 tcagtccgcg gacaaagagt gaaataacgg atataattat tattgaggaa gatatggtat
     1141 aaaacaattt tctctttccg atgaaatcga aactgacacc tctcaggaaa ttctccgtcc
     1201 actttataga gaaggtcagc cgggcatttc tcttcagcag gccttcataa atgagcctgg
     1261 aaatgtatat ggcactgaaa agggatgtga atattccgat aaccagcatt gttgcaaatc
     1321 cctttatcgg tccggttccg aaaatataaa gcacgattcc ggtcagcagg gtggtgatgt
     1381 ttgagtccag aattgcggaa agtgatcctt tgtaaccatc ggcaattgcg agcttgactc
     1441 ccttcccggc tctcagttcc tcacgtatcc gctcgaagat cagaacgttc gcatcaactg
     1501 acatacccat tgtcagaaca atccccgcaa tgcctggcag ggtaaatacc gcaccgagtg
     1561 atgcggaaac acccatgata aggaaaatat tgctgaaaag ggctatgtcg gcaactcccc
     1621 cggcgcttct gctgtagtag aaagccatgt aggcaagaac aatgacgaag gcaatgatga
     1681 atgaaatcaa tccggcattg atggcttcac gtcccagcgt gggtccgaca accgtgtccg
     1741 aaacgattcg tgctggagca ggcagcttgc ctgatttaag gatattggcc aggtcgtcgg
     1801 cttcctcaat ggtaaaatcc ccggttatct cggtgcttcc ggtgggaatc tcgcctacca
     1861 cgcgcggata ggacctgaca tagccgtcaa ggaccacggc aatacacctg tttacatttt
     1921 cacgggtcat ccttgcccat gtttttgctc cctccgaatt catgttgaag tcaaccttca
     1981 cttcactgcc ggtcatcccg gaggtggggc gtgcggatgt tataacactg ccgtcaaggg
     2041 gagcccgccc gtcacgggtg gtaaccttta ttgcatgaag ctcatacagg cttcccgacg
     2101 gatcgtattt gtgagggttc tggctccagt acagcctcag gtccctgggg aaaagagccc
     2161 gtacctggtt catggcaaga tacctgttga cctttgcggt atcaaccccc aaagccagac
     2221 ctatcataca ggaaggaagg gcttccccct gctcggtgac ccttggtgtg agtatgccga
     2281 aaagcgggtt ctgaagtgcg aattcctccc gggtggatgc agcggctgtc tgaactgtat
     2341 ctttctcaat gagatccagg agatcctgta tttcactgcc gctgctttcc gaagcagctt
     2401 ctgctgctgc gccctgtttt tcagcttcaa cagcctgcct ggcatttgcc tcaatacctt
     2461 tcagcagctc attcgcggaa ataagatagt ttattatttc actgttttca tatgtttccc
     2521 agaattcaag attggccgta ccctgaagaa gctcacggac cctctgggga tcagtctggc
     2581 ccggcagttc gataagtatt ctccccttcg ttgcaagctg tgttatattg ggctgtacaa
     2641 ccccgaacct gtcaatcctg gttctgagaa tattgaaggc attcttggtt gcactgttga
     2701 tttcctcgtc aagaacccta agaacctgct cattggttga attgaaattg atcttgtccc
     2761 tcaggtcaac cgtactgaaa atggctgcca gttttgcatt cggatcaatt tcctgaaatg
     2821 cccgtccgaa aagaacaaga aaatcagcct ggctttcttt ctcaagctcc tttgcccttg
     2881 ccagagcact taaaaatgtt ttgtccgtac tgtgatttga aagtgccttg aggatatcct
     2941 caacggacac ttccagtatg acgttcatac cgcctttaag gtcaagcccg agattgatct
     3001 ccctcttctg gcagtccttg aaactgtagc caaggaaact ccatttgctt tttggcaatg
     3061 aggctattga atcaatgtaa tacgtctgtt tcaccaggtc tccctgagca tatgttttgg
     3121 catcctgttt aaccttgtag gttgcaccgg tgaatgaaag ctggtaaacc gacaccaggg
     3181 caagagcaat ggcaagaacc attatggctg ttttgttctg catttgtatg gcttttgaat
     3241 tataccgtgt ttgttttcct cttattaata attgaggcgc aaatatatta aaattttatg
     3301 gaaaaaaggc tgaataaaat ttatccagcc ttgcagtatg aatgagtatt gctgttacta
     3361 atcaagcagt ttttcgtagt ttgtctcgag gggccgcctg ttcagaaaat cgtaaagggc
     3421 aaggtttctt aaattgtaga aatacgacca gtaattctgc aatgcctgaa catatgccct
     3481 cctgttggtg tctttccggg tatcggcgtt gttcagctca agcacctgta tctttcctat
     3541 cagaaagcgg gcctttgtga cttcatacat tcgtgcggcg acagtatcag attttgcagc
     3601 aatcctcacc tgctccgcct gaagattgaa ctgttcaaca tccagaatca ggttctggtc
     3661 aaagtcaacc tgatcctgtc tcgactggac ctgtgccagt tcaaggctgg attttgccat
     3721 caggtatctc cccctgccca gtccccagtc aagtatgggc agtgttatcc ccagccttat
     3781 agtctgctga cggttggatt cctgataggc atagcggaaa accgggtcct ggtcacgcag
     3841 accgaaagag gcaatgaggt tggcattaag tcccttgtca gctttagcct gggccaggtt
     3901 gctctgtgcc gtaagaacat tgagctgctg attgattatc tgcgggttgt tgttgtgagc
     3961 caggtcaaga acctcctgaa catcaacctg aagatccggc actgacgacg gaagtatcag
     4021 ttcaagtctt atattttcat tgaagccgag aaaggacctg agtcttaatt ccctgtcctt
     4081 gagattcatg tcagcttcct tccgtgcagt ttcggagttg agccaggaaa gctgcatctg
     4141 aagcagctcg tcttcagcga tggtcccgag attgaaccgg ccctgtgcca ttctgtacaa
     4201 tgtatcggca ttcagataat tcgtctctgc aatctgcttg tttatctgtg caagggtcag
     4261 gctgaaaaac tgccgtacag cttccatgtg caccgattca atattggaga ggaaggtttt
     4321 ttttgccgct tcatatttaa gaggttctgt ctttttctgc catcccaggg aattgtatct
     4381 gaataaaggc tgtacaaacc ttacgctcag gggggctgta atatatctgt ttcttgtctg
     4441 atccttcgcc ttgtcaagat ccgtttcaag agtaagatcc gactgcaggg caatcgttcc
     4501 gcccgtaatg ccgatattct gcagaaggga cagggagccg attgtctgga ggatgtttgt
     4561 tgagacataa taccactcgc ctgcctggga attccatacc ctgctgtaag cggtactgta
     4621 atcgggggtg gttcccgtca gggtcagtga tggtctgtac tgggccacat aagtccggta
     4681 ttgccagtag cttgcccgga agcggtgttt cgccataagg gccaggggtg actgatcctc
     4741 agccagttta attacttcgc tgagggtgag cttcctgatt tcctgtgctg taactgattt
     4801 tattgagaga aggaatgaga ataccactac cgggataatc agccggttca ttactagagt
     4861 tttattcata tcttaatgat tctataggat ctttttccga tgcacgcttg gccggagagt
     4921 agccaaaaac cacacccacg gctgctgata ctccgaatgc tataaaaacg gagaaaaaag
     4981 aaacaattgt cataatcccc gcaacctgtt ctattatttt tgccatgatg acacccagga
     5041 atattccgac gaatccgcct gaaacgctta tcagaacagc ttcggaaaga aactgtacca
     5101 cgatatccat cttttttgcn nnnnnnnnnt tgcaccgatt gccatcctgg tcccgatttc
     5161 ctttattctt tccatcactg atgcaaacat gatattcatg atgcctatgc ctcccactac
     5221 aagtgaaatg ctggcaattg caccgagtac aatattgaat atatccttgg ttctctgctg
     5281 ctgtttaagg agcagctcag gcactgttat ctcaaaatcc tttacatttg aatggcgcct
     5341 gatcatcata cggctcagta tttccgttga aggagtaatc tgttctgttt catttacctg
     5401 caccacaata cggtcaagct ggttatagtt tgtctccaga tccgatcctg catcggaact
     5461 gcctgaaagc acccttccca ttccgccgcc aaagcccccc attacaaaaa cattcgatga
     5521 ggcttccgaa gccagttttg tattcacgag ggccctgttc tggtatctga gaagcatggt
     5581 ctgaatgggg atgtaaatat tgtcattgta cacattcaca cccttttcct caaacccggt
     5641 caggcttacg gtggttttct gaagaactcc gatgaccttc agccatatgc cgttgaattt
     5701 gatatactgc ccgatcggat ctaccttgct gaaaaacttg gcccttatat ttgccccgat
     5761 aatgcagacc tggataccat tttcttcctg gtaggaattg aagaaggcac ctgaaaccag
     5821 cggcagattg tagagatgga aataatcatt tgaaacaccg acgagcttgg cagtatattt
     5881 aacaccgttc attactgctg ttgaattgaa ggatatctcc ggactgatcc gcttgacgga
     5941 aggcagggtt tcattgatcg cttccacgtc aagcatgctc agccccctgg agaatttctt
     6001 tgcctgcttt tcgccttcct caccacctga cgactgatcc tgcacgatgg gattgatgag
     6061 gatattattc actcccacca ttttcatctg ttccattatc tcctgcttgg cacccttgcc
     6121 tatggccagc atggcaatca cggcagccac cccgaatatg ataccgagag ctgtcagcat
     6181 ggatttcagc ttgtttgaga ttatcgattc aacggcaatc tcaatatcat gaaaatatct
     6241 gaaaataaac ttaaacattt ccggccttta tttattgcta tacaatttac tggttgcgct
     6301 gaggctgccg gggtccttcc cctccctgct ggttcccggg tctttgctga ccctggggag
     6361 tatttccgcg
//
LOCUS       DAOSPX010000181         6369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11237_length_6369_cov_3.289832, whole genome shotgun sequence.
ACCESSION   DAOSPX010000181 DAOSPX010000000
VERSION     DAOSPX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11237_length_6369_cov_3.289832"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLZ75_09215"
     CDS             complement(<1..648)
                     /locus_tag="PLZ75_09215"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF017903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOS72610.1"
                     /translation="MKKETIEKAYKLAKDQYAELGIDTDKALSALSEIVISLHCWQTD
                     DVGGFEKEGAELGGGGIQATGNYPGKAKTIGQMRDDLDKAMSLIPGKKRLNLHASYGE
                     FGGKLTDRDAIEVKHYQGWIDWAKERNTGLDFNCTCFSHPLADDGFTLSSKNEKTRAF
                     WVEHVKRCRAIGAEMGKQLGTPTVHNLWIPDGSKDIPVDRYGVRKILKKSLDEIFL"
     gene            complement(668..985)
                     /gene="rhaM"
                     /locus_tag="PLZ75_09220"
     CDS             complement(668..985)
                     /gene="rhaM"
                     /locus_tag="PLZ75_09220"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOS72611.1"
                     /translation="MKKRFGFKMKLFPGFREEYRKRHSEIWPELVKLLREAGIGNYSI
                     WYDEETDFLFAYQEQTGESSSQDLGTTEIVQKWWKYMADIMETNPDSSPVSVPLEEVF
                     YME"
     gene            complement(1047..2075)
                     /gene="rhaT"
                     /locus_tag="PLZ75_09225"
     CDS             complement(1047..2075)
                     /gene="rhaT"
                     /locus_tag="PLZ75_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOS72612.1"
                     /translation="MDPLSGIFLIALGSIGAASFYVPFKKVKSWAWESYWISQGFFAW
                     IVIPWIFAFIFVPRGELIPIIKESPASVKFMVAFFGMLWGFGGLTFGLALRYLGIALG
                     QSIALGLCAAFGTLLPPVIAGENLFSTKGGILTVIGVAVTVAGIIIIGYAGSLKSREM
                     PEEDKKAAVREFALKKGILIAVFAGIMSACFNFGYEAGKPMEEIALAHGTNPLYQKNP
                     SLIFILLGGFLTNLVYCVFLNIKNKTYKDYYTVSGGIFANNLVFTFLAGLLWFLQFHF
                     FGMGSSKLPAGMAIFGWSILMALNISISNIWGIILREWKGVSAKTIAVLITGIVVLIL
                     SAFLVKLG"
     gene            2197..4938
                     /locus_tag="PLZ75_09230"
     CDS             2197..4938
                     /locus_tag="PLZ75_09230"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HOS72613.1"
                     /translation="MKSIKISLLLMILTACLSAQNLAVRDLRCEYKINPEGIDVLQPR
                     FSWKINGTGNNILQTAYSLRIATDPRFSPKSIVWESGRVASDESHLLAYKGPDLKSGQ
                     RYYWQVRIWDNKGRSTRWSETAYWETGLLSPSDWKAKWIELDDDTVRYAPSPHFRKEF
                     TVSKPVRKATVYVSAHGLYELHCNGTKVGDQVMTPGWTSYGKRLQYQVYDVTPQITRG
                     RNAIGAFLGDGWYRGTLAWGNNWAVYGKRLGLLMQLKIVHTDGTETMIITDESWKGHN
                     DPAIRMSDIYNGFVYDATKRLTGWSSPGYDDSSWKKVSTGSYSNNLIASEGAPIRKIE
                     EIKPLKVFRSPKGSLLVDMGQNMVGWIRLKARGPRGTVITVRHAEILDKYGEFYTENL
                     RGAKARLTLTLAGTGEDEVFEPRFTFMGFRYLEINGFPGTPGTDDITGIVVHSDMEKS
                     GSYESSNPLINQLQHNIVWGQKGNFLDVPTDCPQRDERLGWTGDVVAFCRTAAFNFNV
                     APFFTKWLKDLALDQKQGGEVPDVIPDVLNPQNAVSAQPSAGWGDAAVITPWTMYLVY
                     GDKRLLETQYPSMKRWVEYIRNKAGDTYQWKGGSKYGDWLFYHPPVNNHTAADGYTEP
                     DFIATAFFANSASLLAQAAAELGKTEDEKTYKELFNKIREVFIREYVTPAGRVGTCSQ
                     TSYILALKFNLLPDNLRENAARFLAEDIKKRGNHLSTGFLGTIFICHELTNAGYTDVA
                     YDLLLQETYPSWIYPVKMGATTIWERWDGQKPDSTFQDAGMNSFNHYAYGAIGDWMYR
                     VSAGIETGTPGYRHLLLQPHPTKRMDFARASYESPYGTVASGWERKDGKITVRVKIPA
                     NSDAVVFLPVDDPAKITQNGMPLTEQEKQNLISEETREGKRIGIAKGSGEYTFEYTE"
     gene            4964..5869
                     /locus_tag="PLZ75_09235"
     CDS             4964..5869
                     /locus_tag="PLZ75_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOS72614.1"
                     /translation="MGFIENHTGEFAALLTAVFWAVSALAFETATIRLGSVAVNILRL
                     ILGFMFLAILNAVRRGLPFPSDAGIDNWFWLILSGLVGFVFGDLFLFKSYAIIGSRFS
                     MLIMTLVPPVTAFFGWLMLGEHLTLLNLLGMILTFSGIGLAILTRDGSKKEKLTLKLK
                     PVGVLYAFGGTLGQALGLILSKMGMKDYDPFASTQIRIIAGIGGFAVLITFMKRWTSV
                     IVSLRDKAGMKALTVGALFGPFLGVSFSLVAVQLTKAGIASTIMALTPVFILLPAVFL
                     YRQKVTLPEIAGALISVSGVALFFL"
     gene            6131..>6369
                     /locus_tag="PLZ75_09240"
     CDS             6131..>6369
                     /locus_tag="PLZ75_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72615.1"
                     /translation="MNANNNEATALRGEGRLVGRSSDEEPGAKTMFFSQNEIQTQLVS
                     NRSTDFRNRFFPLTTIDSWNDWKWQLRNSITTIDEL"
BASE COUNT         1746 a         1509 c         1470 g         1644 t
ORIGIN      
        1 taaaaatatt tcgtcaagcg acttcttcag tattttgcgg acaccgtacc tgtcaacggg
       61 tatgtccttt gatccatccg gtatccacag attatgtacg gttggggttc ccagctgttt
      121 tcccatttca gctccgatgg cccggcatcg tttcacatgc tctacccaga aggcacgggt
      181 cttctcgttc ttacttgaaa gagtgaatcc gtcgtcggcc aggggatgag aaaaacaggt
      241 acagttgaaa tcgagtcctg tattcctctc ctttgcccag tcaatccatc cctggtaatg
      301 ctttacttct atcgcgtcgc ggtcagtcag tttcccaccg aattccccgt agctggcatg
      361 aaggttaagt ctcttttttc ccggaataag cgacattgcc ttgtccagat catctctcat
      421 ctgaccgatg gtttttgcct tgccgggata atttcccgta gcctgtatgc cgccaccccc
      481 aagttcggct ccttcctttt caaaaccgcc tacatcatcc gtctgccagc agtgcagact
      541 gataactatt tccgaaaggg cggacagtgc cttatcggtg tcaatgccaa gctctgcata
      601 ctggtccttt gcaagtttat aagccttttc aattgtctct tttttcatat tccttaaata
      661 tttatgatta ttccatatag aatacttctt caagcggaac acttaccggg gaactgtccg
      721 gattcgtttc cattatgtca gccatgtatt tccaccattt ctgaacaatt tccgtggttc
      781 ccagatcctg ggatgagctt tccccggtct gttcctggta ggcgaacaga aaatcagtct
      841 cctcgtcgta ccagatggaa taattcccta tgccagcctc acggaggagt tttacaagtt
      901 ccggccatat ttcactatgt cttttgcggt attcttccct gaacccggga aaaagtttca
      961 ttttgaatcc aaatcttttt ttcatcgtaa cagaattttt tgcatgagaa tctgtgtccg
     1021 gccagcggct gctgcacatg gacaatctaa cccagtttaa ccaggaaggc tgaaaggatg
     1081 agaacaacga tcccggttat gagcactgca atcgtttttg ctgaaacgcc cttccattcc
     1141 ctgagaatta tcccccatat attgctgatt gatatattca gtgccatgag aatgctccat
     1201 ccgaagattg ccattccggc cggcagcttg ctggaaccca ttccgaaaaa atgaaactgc
     1261 aggaaccata gcagtccggc aagaaaggtg aataccagat tattggcaaa tatgccgccc
     1321 gaaacggtat aatagtcctt atatgttttg ttttttatat tcaggaatac acagtaaacc
     1381 aggttggtca gaaatcctcc cagaagaatg aaaatcaggg aggggttttt ctgatagagg
     1441 ggatttgttc cgtgagccag ggcaatctct tccataggct ttcctgcttc atagccgaaa
     1501 ttgaaacagg cactcatgat tccggcaaac acagctatca gaatcccttt cttcagggca
     1561 aactccctca cggcagcttt tttgtcctcc tcaggcattt ccctgctttt cagggaaccg
     1621 gcatatccga ttatgattat tcccgcaaca gtgacagcca cgcctatcac tgtaaggata
     1681 cccccctttg tggagaaaag attttctccc gcaattaccg gcggaagaag agttccgaag
     1741 gcagcgcaaa gacccagcgc tatgctctgt ccgagagcta ttcccagata tctgagtgca
     1801 agtccgaatg taagtccgcc aaatccccag agcatgccga aaaatgctac cataaacttc
     1861 acagatgcag gggattcctt aataattggt atcagttcgc ctcggggaac gaatataaag
     1921 gcaaaaatcc agggaatgac aatccatgca aaaaatccct ggctgatcca gtaggattcc
     1981 caggcccagg atttgacttt tttaaaggga acataaaaac tggcagcccc gatactgccc
     2041 agcgcaatga gaaaaatacc actaagcgga tccatatttg agtttttctt gaagtgatcc
     2101 gcaatttaat aactttcagt caggaaaact gcggatcagt ttttttattc acacaaattt
     2161 tataatcttg ggatttatta cctaaaatcc ccgatcatga aatccataaa aatttctctt
     2221 ctgctgatga tcttaactgc atgtctttct gcacagaatc ttgcagtcag ggacctcagg
     2281 tgtgaataca aaatcaatcc tgagggcatt gatgtattgc agccgcgttt ctcatggaag
     2341 ataaacggca cagggaacaa cattctccag acggcctatt cgctcaggat tgccaccgat
     2401 ccccgctttt ctccaaaaag tattgtctgg gaatcaggca gggttgcttc agacgaatcc
     2461 catctgcttg cctataaagg accggatctg aaatcagggc agcggtatta ctggcaggta
     2521 agaatatggg ataacaaggg ccggtcaaca cgctggagcg aaaccgccta ctgggagacg
     2581 ggccttcttt ccccttccga ctggaaggca aagtggattg aacttgatga tgacactgtc
     2641 agatatgctc catcacctca tttccggaaa gaattcacag tgtcgaaacc ggtcaggaaa
     2701 gcgacggtat atgtcagtgc acacggatta tatgaacttc actgcaatgg cacaaaggtg
     2761 ggggaccagg taatgacacc gggctggaca tcctacggga agaggcttca gtatcaggta
     2821 tatgatgtta caccgcagat caccagaggc agaaatgcca tcggtgcttt tctgggtgac
     2881 gggtggtaca ggggtacact tgcatggggc aataactggg ctgtctacgg aaaaaggctg
     2941 ggattgctta tgcagctgaa aattgttcat acggacggaa ctgaaacaat gatcattacc
     3001 gatgaatcat ggaagggaca taatgatccg gccatccgta tgtctgatat ttacaacggc
     3061 tttgtctatg atgcgacaaa gcggctgaca ggctggagca gtccgggtta tgacgacagc
     3121 agctggaaaa aggtgagcac cggtagttat agtaataatc tgattgcctc tgaaggagct
     3181 ccgatcagga aaatcgaaga gatcaaaccc ttaaaggtgt tcaggtctcc caaaggcagc
     3241 cttctggttg acatgggtca gaatatggtc ggatggataa gactgaaagc cagagggccc
     3301 cggggcacag tcatcacggt acgtcatgct gaaattcttg ataaatacgg tgaattctat
     3361 acggaaaatc tcagaggcgc caaggcccgg ctcacactga cactcgccgg aacaggtgag
     3421 gatgaggtgt ttgaaccgcg ctttaccttc atgggattca gatacctgga aataaacggc
     3481 ttccccggaa cccccggcac tgatgacatt acgggaatcg ttgttcattc cgacatggag
     3541 aaatccggaa gctatgaaag ctcaaatcct cttattaatc agttgcagca taacatagta
     3601 tggggacaaa aaggaaattt tctggatgtt cccaccgatt gcccacaaag ggatgaacgc
     3661 ctcggatgga ccggtgatgt agttgctttc tgcaggactg cagcctttaa tttcaatgtt
     3721 gctcccttct ttacaaagtg gctgaaggat cttgcacttg atcagaaaca gggaggcgag
     3781 gttcctgatg tgattcccga tgtccttaac cctcagaacg ctgtcagcgc acagccttca
     3841 gcaggctggg gggacgcggc tgtcatcaca ccatggacca tgtatctcgt ttacggggat
     3901 aagagattgc tggaaacaca atatccgagc atgaaaagat gggttgaata catccgtaat
     3961 aaagccgggg atacttacca gtggaagggc ggcagcaaat atggcgactg gctcttttat
     4021 catcccccgg taaacaatca taccgccgct gacggatata ctgaacccga tttcattgca
     4081 acagcctttt ttgccaattc tgcaagccta ctagcacagg ctgccgctga actcggaaaa
     4141 acggaagatg aaaaaaccta taaggaactg ttcaataaaa taagggaagt gtttatcagg
     4201 gaatatgtaa caccggcagg aagggtcggc acatgttcac agacttccta tatactggcg
     4261 cttaaattca atctcctgcc tgataacctg cgggaaaatg cagcacgctt ccttgcagag
     4321 gatatcaaaa aaagaggaaa ccatctgtca accggttttc ttggtaccat attcatctgt
     4381 catgaactga caaatgcagg atatactgat gttgcctatg atctccttct ccaggaaact
     4441 tacccctcat ggatatatcc ggttaaaatg ggtgccacaa ccatctggga aagatgggac
     4501 ggacagaagc ccgacagtac ttttcaggat gcgggaatga actccttcaa tcattatgct
     4561 tacggtgcga taggcgactg gatgtacagg gtgtctgccg gcattgaaac cggaacaccg
     4621 ggatacaggc atctcctgct gcagcctcat ccgacaaaaa ggatggattt tgccagggca
     4681 agctatgagt ccccctacgg gacggttgcc tcgggctggg aaagaaaaga cggaaagata
     4741 actgtaaggg taaaaatccc ggcaaactcc gatgctgttg tattccttcc ggttgacgat
     4801 cctgcaaaaa taacacagaa tggtatgcca ctgactgagc aggagaaaca aaacctgatc
     4861 agtgaagaaa ccagggaagg caaaagaatt ggcattgcaa aaggatcagg tgagtatacc
     4921 tttgaataca ccgaatagaa ccgaaacctg acacactctc cggatgggat tcatagaaaa
     4981 tcataccgga gagtttgctg ctctccttac ggctgttttc tgggcagtaa gtgctcttgc
     5041 ctttgaaacc gcaacgatca gactgggatc tgtagcggtt aacattctca ggcttattct
     5101 gggttttatg ttccttgcaa ttctcaatgc cgtcagaagg ggacttccgt ttccttcaga
     5161 tgccggcatc gacaactggt tctggctaat actgtcggga ctggtaggat ttgtattcgg
     5221 tgacctcttc ctgttcaaat catatgcaat tatcggctca aggttttcaa tgctgataat
     5281 gaccctggtg ccccccgtca cagcattttt cggatggtta atgctgggag agcatctcac
     5341 cctgctgaat ctgctgggta tgatccttac attttccggc ataggtctgg ccattcttac
     5401 cagggatggc agcaaaaagg aaaagcttac actgaaactc aagccggtcg gggttctcta
     5461 tgccttcggc ggcactctgg gtcaggcact cggactgatt ctcagcaaaa tgggaatgaa
     5521 ggattatgat cccttcgcat cgacacagat acggataatc gcaggaatag gaggattcgc
     5581 agtactgata acctttatga aaagatggac aagtgtgatt gtttcactca gggataaggc
     5641 cggaatgaaa gcactgaccg tcggagcgct tttcggaccc ttcctcgggg tatcattttc
     5701 acttgttgcc gtacaactca caaaagccgg aatcgcttca accatcatgg cgctgacccc
     5761 ggtgtttatc cttttaccgg ccgttttcct gtacaggcag aaagtgacat tgcctgaaat
     5821 tgccggagcc ctgataagcg tcagcggcgt tgccctgttt tttctttaaa caagaagaat
     5881 tccggacaca ggagttccgt atccttacat cagtcccaac atatagtctt acagcgaatt
     5941 aacaggccac taaaggctta tgacattaaa gctgttgata atttgaatcc cctgttaata
     6001 tttatggctt acaggtgata caacgtatta tcagattata tactttcgca tcattaaaaa
     6061 aagtactagt aataatttaa aaccacatta tctattaaaa acaaacagat gaattatttc
     6121 atctccagcc atgaatgcta acaataatga ggctacggcg ctccgcggtg agggccgcct
     6181 cgtgggtcgt tcttccgatg aagagcctgg cgcgaaaacc atgttttttt cgcaaaacga
     6241 gatccaaact cagttagtta gcaacagaag cactgacttc agaaaccgct tcttccccct
     6301 aacgactatc gattcctgga acgattggaa atggcagctc agaaattcaa ttacaacaat
     6361 tgatgagct
//
LOCUS       DAOSPX010000182         6352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11273_length_6352_cov_4.151660, whole genome shotgun sequence.
ACCESSION   DAOSPX010000182 DAOSPX010000000
VERSION     DAOSPX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11273_length_6352_cov_4.151660"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="PLZ75_09245"
     CDS             <1..1445
                     /locus_tag="PLZ75_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72616.1"
                     /translation="HPLGHNDHLYRGDHIAFYDYTADFASGRPRDRIPSVEGKGTVTI
                     RNGTIRNAATGILSWGIQSTAENVKIILDNVRIVSSGINTVAADLGQAVISDCSFEID
                     NPFIINRHGSSFYAVDLTGKQPSQVSYCKFLGGQGCLSFKGDFSAVHHNHFINRQTVT
                     NHYSIMAMGDSSLIFENTFEPEIGSGIEVYVHRGIEIFNNIFHISAAPPSCEYNTHLS
                     TNAIRLADYGAEKGSPRGCYANRVYNNKFFIRGRKFRNHPGFVPVANAVFYSASGGDN
                     EIFGNEIIVHQEDPETDAEAFAFYIGNSNGGSIYSNRIISNVTPVWVACSYGGAENTV
                     FTGNIFQKAENTPAAAFPAVRMGSAEDSGCIAEGTEFRSNQFSGNSFSVDATGQPHSY
                     AVYWKLQINLKDRNGRPLTDYEVVVADRQGREVSRRKSDKNGILMLELPEYTVNGPVK
                     TDFSPYLIKAGKRKTETVLDRNRTIDLTVR"
     gene            1603..4803
                     /locus_tag="PLZ75_09250"
     CDS             1603..4803
                     /locus_tag="PLZ75_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOS72617.1"
                     /translation="MKTSAAFMIILGLLCQAGYSQEKTVAKIALVQTSARTNQDPFMA
                     DYEPGRVRPLMIENFENILGMYEKAGRMNADLVCGPEDIQNIGSYGLYIDKTDPETGK
                     ILFSSMALKIPGPFTDRLADIARKYRMYIIAPLYEEEDGRIYNTALLFDRQGNIIGKH
                     RKTVLPVMETWLVDTGNEFEVFQTDFAAIAIATCWEMFYPEIATIYALKGADIIFNPT
                     MALDNKPGHGLETASTYITRARDNSVYIAPVILGTEGTGIIDFHGNVIAEAYGEKDRI
                     IMTEIDFGKERISGSEWWKTINGTDNTKAIHFKSRRPELYKMITEANPPVLDRYRDIR
                     LTTGDRRRQLEAVRKVDYGPGSGSAADTEPGSLSDISELKQLGLDVIPYPREVTIEGE
                     DFIINEGLMLVLDRNAGKSDRFTAEELIHDLNKKFSISARIGNDGSRPAVVLTRHRVP
                     RSLPAQGYQITVSANRIEIKARSDDGLFYGTQTLLQLIRKEGSVRKVPGMIITDWPDI
                     KNRAVHYDTKHHQDKASYVKDFIREVAYYKINMLVWEWEDKFAYPSHPEIGAPGAFTM
                     EEMQEFTRYARKYHVQIIPLVQGLGHVSYILKWPQHRHLREIEASNWEFCPLKEGSYD
                     LLFDLWKDAIEATPGSEYIHIGSDETYELGSCEQCRAKSEEIGKSGLYHFFIKKSGQF
                     LQSKGRKVMAWESPMGWTRGRSPAKGIEPLKGLILTESYDYETPDLKYVREARSRGFE
                     TYAYDPNPEVVPMMVPYFFEKGEKEGLRTGSLEKSFGFLSHAATSGVFGGMICTSWDD
                     DGLHNQMWMMHFINAAARSWNGNKPSLREFTESYFRNYYGTEASDMEELFRLLNEAVH
                     YFARTMERNVWHYGEIGKTRLPDLPRGDALEYDPFWNTEYREKVGESAEILEKMNRAL
                     EIIRKNIESGADHKYDFEIFRTTAELVKHTSLTFLDLSDLENTIRTAHVNRFVNTGVS
                     IDNLLKAQQILENLLERRETVFNDLVKTYETTRFPKGYSTAGKEFFWQQDRARHFAFR
                     RPDMSFLIWDEQLLDIEGYLEKLKNYTEFFREHGTK"
     gene            5119..6264
                     /locus_tag="PLZ75_09255"
     CDS             5119..6264
                     /locus_tag="PLZ75_09255"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS72618.1"
                     /translation="MINRREFLKISGMGAAGLSLSGMTGCGTGTSGRGIGPVGVKVID
                     AHIHATPGRIGRTLEIMDDNNIRYALLIASISGTDDNLYVGDKAFYELIEAIRPYRNR
                     LGVHYTYDWTLAETDPDFFSKAPDMLEKAVNAGAIALKNLKDLGLNARDTEGRLIAID
                     DPRLFPIWERAEKLGISVAFHVGDPVAFFQPWEPGNERWDELKLHPEWSFADRSKYPP
                     LETLFEQVKNVYRKFSGVRFVGVHVAGYSENIKAVGSWLDELPNLSIDTAARIGELGR
                     HPAADGHDFFTKYHDRIMFGTDLAFWPDCDVQGAGPCENFSTEEHRKFYEIHWRYFQT
                     DDRQFDHPTPIQGNWKIDGIRLDEKTLRELYWDNAYRFYDLGRFNVS"
BASE COUNT         1955 a         1430 c         1533 g         1434 t
ORIGIN      
        1 cacatcccct gggacacaat gatcatctgt accggggaga tcacattgca ttctatgatt
       61 atactgctga ttttgcatca ggcaggccgc gtgacagaat accttccgtg gaagggaaag
      121 gcactgtaac gatcagaaac ggtaccatac ggaatgcggc aacaggcatt ctgtcatggg
      181 gaatccagtc aaccgcggaa aatgtgaaga tcatactcga caatgtcaga atcgtttcct
      241 cgggaataaa taccgttgca gctgacttgg gccaggcggt gatttcggac tgcagctttg
      301 aaatcgacaa ccccttcatc atcaaccgtc acggatcatc attctatgca gttgacctga
      361 ccgggaaaca gccctcgcag gtttcttact gcaaattcct cggaggacag ggctgtctgt
      421 cctttaaagg agatttttcg gctgtacacc ataaccattt cataaaccgc cagaccgtca
      481 ccaatcatta cagcataatg gcaatgggcg acagttccct tatatttgaa aataccttcg
      541 aaccggaaat cggatccggc attgaagtat acgttcacag gggaatagaa atattcaata
      601 atatattcca tataagcgca gcacctccca gctgtgaata taacacacat ctcagcacaa
      661 atgccatccg ccttgcagac tatggtgccg aaaaaggatc cccgcgaggg tgttatgcca
      721 accgtgtcta taacaataaa ttttttatca ggggaagaaa attcagaaac caccccgggt
      781 ttgttccggt ggcaaatgca gtcttttaca gcgccagcgg aggggataat gaaatcttcg
      841 gcaatgaaat cattgtccac caggaagatc cggaaacaga tgcggaggct ttcgcatttt
      901 atatcggcaa ttcaaacggt ggcagcatat acagcaaccg gattatttcc aacgttaccc
      961 cggtatgggt tgcatgttcc tatgggggag ctgaaaacac cgtgttcaca gggaatattt
     1021 ttcaaaaagc agaaaacact ccggcagccg ctttcccggc cgtcagaatg ggatctgccg
     1081 aagactctgg ctgtatcgct gaaggaacag agttccgctc caatcaattc agcggaaaca
     1141 gcttcagtgt tgatgcaacc ggccagcccc attcctatgc ggtttactgg aaattgcaaa
     1201 taaacctgaa agacaggaac ggcaggcctc ttacggatta cgaagtggtg gttgctgaca
     1261 gacagggcag ggaggtatca cgccggaaaa gcgataagaa cggaatcctc atgctggagc
     1321 ttccagaata taccgtcaat ggccctgtta aaacagattt ttcaccctac ctcataaagg
     1381 ccggaaaaag gaaaacagaa acagttctgg accgtaacag gacaatagat ctgactgtca
     1441 gataagcttt ccccggaaag cggacattag tgaaaccggt ttttttaaag catttgtcat
     1501 ttaattcctt tacaattacc ataagttctg attaaatgca taatttgtgg cagtctgttt
     1561 caggacagta acaaacaaca gctgtatcaa aaaaatccag gaatgaaaac atcagcagct
     1621 ttcatgataa tcctgggcct cctgtgccag gcaggatact cccaggaaaa gacagtggca
     1681 aagattgcgc ttgtccagac cagcgccaga acaaatcagg atccttttat ggcagactac
     1741 gaacccggca gagtccgccc ccttatgatt gagaactttg aaaacatact gggaatgtat
     1801 gaaaaagccg gccggatgaa tgcagatctt gtatgcggac cggaagatat tcagaacatt
     1861 ggttcatacg ggctctacat tgacaaaaca gatccggaaa cgggaaaaat cctgttcagc
     1921 tccatggccc tgaaaatacc gggtcctttc accgacaggc ttgcagacat agccaggaag
     1981 tacagaatgt acatcattgc ccccctctat gaagaggaag acgggagaat atacaacacg
     2041 gccctccttt ttgaccggca gggaaacatt atcggaaagc acaggaaaac cgtcctgcct
     2101 gtcatggaaa cctggctggt tgacaccgga aatgaattcg aagtgtttca gaccgacttt
     2161 gctgccattg caatagcaac atgctgggaa atgttttatc ccgagatagc aacaatatat
     2221 gccctgaagg gtgccgatat cattttcaat ccgacaatgg cactggacaa taaaccagga
     2281 cacggactcg aaactgcttc aacctatatc acgagggcaa gggataattc ggtatatatt
     2341 gcacctgtga tactgggaac ggaaggaaca ggcatcatag atttccatgg aaacgtgatt
     2401 gctgaggcat acggagagaa agacagaatt atcatgaccg aaatcgactt cggaaaggaa
     2461 agaatcagcg gaagtgaatg gtggaaaaca ataaacggaa cggacaatac aaaagcgatc
     2521 cacttcaagt cgcgccgtcc ggaactgtat aaaatgataa ctgaggcgaa tccgccggta
     2581 ctggacagat acagggatat ccggcttacg acgggtgacc gccggaggca gctggaagca
     2641 gtaagaaaag tagattacgg tcccggttca ggaagtgctg cagacacaga accgggaagc
     2701 ctgtcggata tcagtgagct gaaacaactg ggactggatg tgatacccta tcccagggaa
     2761 gtcacaattg aaggagaaga tttcattata aacgagggat taatgcttgt tcttgacagg
     2821 aatgcgggga aatcagacag attcacggcg gaagagctga ttcatgattt aaataaaaaa
     2881 ttcagcatca gtgcccgtat cggaaatgac ggcagccgtc cggctgttgt ccttacccgg
     2941 caccgtgttc cccgctccct gccggcccag ggataccaga taacagtatc tgcaaacagg
     3001 atcgagataa aagcccggtc ggatgacgga ctcttttacg gcacccagac attgctgcag
     3061 ctgataagaa aggaaggatc agtccggaag gtgccgggaa tgataattac cgactggccc
     3121 gacattaaaa accgggccgt acactacgat accaagcatc accaggataa ggcctcatat
     3181 gtgaaggatt ttataaggga agtggcatac tataaaataa acatgctggt atgggaatgg
     3241 gaagataagt ttgcctatcc cagccatccc gaaattggtg caccgggtgc ctttaccatg
     3301 gaggaaatgc aggagtttac acgctacgcc aggaaatatc acgttcagat cataccgctt
     3361 gttcagggtc tgggacatgt gagctatata ctcaaatggc ctcagcacag gcatctcagg
     3421 gagatagaag cttcaaactg ggaattctgc cccctgaagg aagggagcta cgatcttctc
     3481 ttcgatctct ggaaagatgc gatagaagcc acaccgggat cagaatatat tcatatcgga
     3541 tccgatgaga catacgaact aggaagctgt gaacaatgca gggcaaaatc cgaagaaatc
     3601 ggaaaaagcg gcttatacca tttttttatc aagaaaagcg gacaattcct ccaatcaaaa
     3661 ggcaggaaag taatggcctg ggaatcccca atgggctgga ccaggggaag atccccggca
     3721 aagggaatcg aacccctcaa agggcttatt ctgacggaaa gctatgacta tgaaactcct
     3781 gatctgaaat atgtcaggga agcacgatcc aggggatttg aaacctatgc atatgacccg
     3841 aatcctgaag ttgtaccaat gatggttccc tatttctttg aaaagggtga aaaggaagga
     3901 ttaagaaccg gcagtctgga gaaatcattc ggatttctct cgcatgcagc aacatccgga
     3961 gtattcggcg gaatgatatg cacttcctgg gatgatgacg gtctgcacaa tcagatgtgg
     4021 atgatgcatt tcatcaatgc tgccgcacgt tcatggaacg gaaacaaacc atcgctcagg
     4081 gaattcacgg aatcctattt ccgtaattat tacggaacag aggcaagcga catggaggaa
     4141 ctgttcaggc ttctgaacga agcagtacat tacttcgcca gaacaatgga aaggaatgtc
     4201 tggcactacg gggaaatcgg aaaaacccgc cttccggacc tgcccagggg tgatgcactg
     4261 gaatatgacc cattctggaa tacggaatac agggaaaagg ttggcgaatc cgcggagata
     4321 cttgaaaaaa tgaacagggc actggaaata ataagaaaaa acattgaatc cggtgctgat
     4381 cataaatatg attttgagat tttccggacc acagcagaac tcgtgaaaca taccagtctg
     4441 acatttcttg acctttcaga ccttgaaaac accattcgca cagcacatgt aaaccgcttt
     4501 gtaaataccg gtgtttcaat tgacaatctc ctgaaagccc agcaaatact ggaaaactta
     4561 ctggaaagaa gggaaactgt ttttaatgat cttgtaaaaa catatgaaac aacaagattt
     4621 cccaagggat acagcacagc cggcaaggaa tttttctggc agcaggacag ggccagacat
     4681 ttcgcattcc gcagacccga catgagcttc ctgatatggg atgagcaatt gctcgacatt
     4741 gagggttatc ttgaaaaact aaaaaattac acggaattct tcagggaaca cggcacaaaa
     4801 taaaccctat cagcagggtt gaaataaaca gagctcccca gatagaacaa aatcctaact
     4861 atcaataata aacggaattc cggaaatgat aacaaatcat ggctggctct gatgccggcc
     4921 atgatttttt aaaacccaaa ccattattga attgtttacc gccgccgata cggtcctctt
     4981 ccccgcatat ccctgtctcc ccttaatgaa acttaaacag ggcaaaacaa agctgatctc
     5041 aggatttata ttaattttat atccgtatag gtggtaattc tgtatcgtta ccggaaaaag
     5101 tccagaaaaa caaataagat gataaacaga agagaatttt taaagatttc aggtatggga
     5161 gcagctggct tatccctgtc aggaatgaca ggctgcggaa caggcacatc cggcaggggc
     5221 atcggtcctg tcggggtaaa ggtaattgat gcacacattc atgcaacacc cggcaggata
     5281 ggcagaaccc tggaaataat ggatgacaac aatatccgtt atgccctgct gatagcatca
     5341 atttcaggga ctgacgacaa cctttatgtc ggggataagg cattttatga gctcattgag
     5401 gccatcagac cttacaggaa ccgtctcggg gttcactata catacgactg gactcttgca
     5461 gaaacggatc ccgatttctt cagcaaggcg cctgatatgc ttgagaaggc ggttaatgcc
     5521 ggagccattg cactcaagaa tctcaaggat ctgggactga atgcaagaga tactgaaggc
     5581 agactgatag caattgatga tccgcgtctc ttccccatct gggaacgtgc cgaaaaactt
     5641 ggtatcagtg tagcctttca tgtaggcgac cctgtagcat ttttccagcc atgggaaccg
     5701 ggaaatgaaa gatgggatga actgaaactg catcctgaat ggtcctttgc agaccggtcg
     5761 aaatatcctc cccttgagac cctgtttgaa caggtgaaaa atgtgtacag gaaattctcc
     5821 ggtgtccgct ttgtaggagt tcatgttgcc ggatattcgg aaaatattaa agcagtcgga
     5881 tcctggctgg atgaactgcc aaacctttcg attgacacgg cagcccggat cggcgaactg
     5941 gggaggcatc ctgccgcgga cggacatgac ttttttacaa aataccatga ccgcatcatg
     6001 ttcgggactg accttgcatt ctggccggat tgtgatgtcc agggagccgg accctgcgag
     6061 aacttcagca cagaggaaca caggaaattc tatgaaatcc actggcgcta tttccagact
     6121 gatgacaggc agttcgacca tcccacacca attcagggca actggaaaat tgacggtatc
     6181 aggctggacg aaaaaacact gagagaactg tactgggata atgcttacag gttctatgat
     6241 ctgggcaggt tcaacgtatc ctgaatgatt cacggaacag tccggcagta agatagatta
     6301 attagtgtct gtccataaag tccctgtttt gtgtaaaatc gggaaaattt tt
//
LOCUS       DAOSPX010000183         6301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11409_length_6301_cov_4.522735, whole genome shotgun sequence.
ACCESSION   DAOSPX010000183 DAOSPX010000000
VERSION     DAOSPX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11409_length_6301_cov_4.522735"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLZ75_09260"
     CDS             complement(<1..866)
                     /locus_tag="PLZ75_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOS72619.1"
                     /translation="MNLKEEKYSRFALVAEMLETPGIVRSFSPAVSERFVSSIKSKKG
                     LMLTGEGSSRIFPAKRAISAMLKKGFSMPVFTEGSTQAREYALGDMAVFASSNSGQTK
                     EVIRLILDLKARNHNAIFGLTANKNTKLEELAHDAHVLSCGKEKAVAATKSVLEQALF
                     YDSLLRNLEGRKMEGLDELAGKLEQVLTSSVSSEITGVIKNADFVYFAGRENGVAGEL
                     GLKANEIIRKKSDFLEGTFALHGIEEVMDKNEVLIWVDPFEADQEQFNECLVKGVGIN
                     IVAISAKQTIFPT"
     gene            complement(892..1662)
                     /locus_tag="PLZ75_09265"
     CDS             complement(892..1662)
                     /locus_tag="PLZ75_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOS72620.1"
                     /translation="MANKFNKVVLSINAHPDDAEAWNAGVLKLLKDKGYKIVIATMTG
                     GDLGGCNTSMEETARIRFEEARKAAAVLDAEYYTLGGVDGFLFDTREMRLKTISLMRK
                     VRAGIVFTHLPNDYHTDHRTTANIVESAAMVASLDPVPVEEKPLDVTPLLYHSSPLTL
                     SDPLGAELNPPHFLVDITSVIEAKKEMLSNHISQIDLMRHMHKIDDFFGFVLEGNRNY
                     GKMAGVEYAEVYWQHLGGGFQKDPQVQEELAEFLVKKL"
     gene            complement(1673..2881)
                     /locus_tag="PLZ75_09270"
     CDS             complement(1673..2881)
                     /locus_tag="PLZ75_09270"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOS72621.1"
                     /translation="MLKGNAIIGQSGGPTSVINSSLAGVIEAGSLSTNIREIYGMHYG
                     IEGFMQEMMFDLGKQSPAVIKGLRGTPSSALGSSRHKVKDEDLPRILSVLKKYNIRYF
                     FLIGGNDTMDTINRVEAYCRDQNYELRGIGIPKTVDNDLFGTDHTPGYASAARTNILN
                     VMQAGILARDMQRVDKFVIYQTIGRDAGWLAAATALARKEEDDAPHLIYTPEFDFDRD
                     KFLTDVEKCIKNYGWVSVVCGEGLKYADGTPVSASATKDKFNNIEFGAMGGTSVGINL
                     HRMISEKFGYRGEFQITESLIMSDFVRASQTDLEEAYLCGVEAVKLANRGETGFMVSI
                     DRMSDEPYMIRFGKAPLKEVAIAAKPMPEDYFNEERNNVSEEFLEYVKPLVGDLPEFT
                     RLEKKMVKIK"
     gene            complement(2906..3559)
                     /locus_tag="PLZ75_09275"
     CDS             complement(2906..3559)
                     /locus_tag="PLZ75_09275"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOS72622.1"
                     /translation="MKVIEVEKLKFSTARPDLDEISLSLDRNVKKEAVDVLNWSDFSY
                     KPDVSFAIAYTDNEILLKYYVTEEYFKAEKTRSNQGVYEDSCVEFFVSPSDDGIYYNF
                     EVNGIGTILLGTGTERANSRTVDPEIISKIRRKSSVGDQPVSERTGNFSWNITIAIPF
                     EVFFRHEVRDLKGKTFRANFYKCGDKLTVPHYVTWNPINTPNPDYHRPEFFGLLKFK"
     gene            complement(3642..4595)
                     /locus_tag="PLZ75_09280"
     CDS             complement(3642..4595)
                     /locus_tag="PLZ75_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOS72623.1"
                     /translation="MKEIVAGVDIGGTNTVYGLVDREGGVVAEGSLNTVSYPEINDFV
                     SALVTAINELLLTGDFKLLGIGIGAPNANYYKGTIELAPNLAWKGIVPLGDLIRAKID
                     VPVIVTNDANAAAMGEMIFGGAKDMKDFIVLTLGTGLGSGIMINGQMVYGHTGFAGEL
                     GHVTIVPGGRECGCGRKGCYETYASASGLVRTVLLLLSEMKEKSILRDIKPSELTSKK
                     IAEAAAANDPVAVKALDFTAEKLAFGIHNAIGFSSPEAVFLFGGLAKAGEMLFEPVRK
                     YVEKNVQPIFRGTVKILPSGIPEKNAAVLGAAALVWNEMLA"
     gene            complement(4615..>6301)
                     /locus_tag="PLZ75_09285"
     CDS             complement(4615..>6301)
                     /locus_tag="PLZ75_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HOS72624.1"
                     /translation="SVPENGARNVRGSRIVISFDEYVVLDKINEKFMVSPPMKRMPEI
                     RIRGKDIVIRYEEELRDSTTYTFYFQDAVRDLNEGNPINNFQFVFSTGPFIDSLSVTG
                     NVLKADDLNPPEATLVVLYIQKEDSAFIREIPSYIAVADKNGYFRIDNVAAREYRLYA
                     LKDADNSKNFNLPDEEIAFLDSTISVTPEKNWIPPGRDTLSLLRQLPGKTKSADTIVL
                     QGDYKLYLFQHEKKIRYLTSSSRGAAYRLNYTLSLPPDSLGFHFSIPGAAPGSYFTEG
                     NKGGDSIRVWIADTAQYNRPLITSVIKYPFTDSSGVNVLREDTVMMRYIFPRTPARGR
                     IKPAPFRFTTNISTASLKPGEALVFTSQTPFRKPDTSRIRLYELEGGDRKKIPFSLDI
                     DSTNSCRISMKGKLGMNKNYIFIADSESFGNIYGEHSDSSAYKFTVKGEESYGKLVLD
                     INNHEGSRIIQLLTEEGKIVREVFMNKDGRLEFPLLDKGVYQVRVIYDLNNDGKWTPG
                     DFHAGRQPEPVSYMPQIIEIKENWVLEYVWDISEKNVKSVRKTPPGTKTRGRM"
BASE COUNT         1524 a         1597 c         1296 g         1884 t
ORIGIN      
        1 gtcgggaaga ttgtctgttt tgcagatata gccacaatat tgattcccac gccttttacc
       61 aggcattcat tgaattgctc ctgatctgct tcaaagggat ccacccatat cagaacctcg
      121 ttcttatcca tcacttcctc aataccgtga agtgcaaaag ttccttcaag gaaatcagac
      181 ttcttcctta ttatttcatt ggctttcagt ccgagttccc cggcaacacc attttccctt
      241 ccggcaaaat atacaaaatc cgcattttta atgactccgg tgatctcact gctgacggat
      301 gaagtaagta cctgttccag tttcccggcc agttcatcca gtccttccat tttccggcct
      361 tccaggtttc tcagaagcga atcatagaac aaagcctgtt caagtaccga ctttgttgca
      421 gccacggctt tttccttgcc gcatgaaagg acatgggcat catgtgccag ttcttccagt
      481 tttgtattct tattggctgt aaggccgaat atggcattat gattccttgc cttcaggtcg
      541 agaatgagtc tgatcacctc cttggtctgc ccggaatttg aggaagcaaa cactgccatg
      601 tcaccgagtg cgtattccct tgcctgggtt gacccctcgg tgaagacagg catgctgaat
      661 cctttcttca gcatggcgga aatggccctt ttggcgggaa atatacggct gctgccctct
      721 cctgtgagca tcaggccctt ttttgatttt attgaactta caaatctttc tgacactgcc
      781 ggactgaaag atcttactat tccgggtgtt tccagcattt ctgccacaag agcaaacctg
      841 ctgtattttt cttctttcag attcatatta tctagattat aatgaacggg attaaagttt
      901 tttcaccaga aattctgcca gttcttcctg tacctgggga tctttctgaa agcctccccc
      961 gaggtgctgc cagtaaactt cagcatattc cactccggcc attttgccgt agttcctgtt
     1021 gccttcaagc acaaagccga agaaatcatc aatcttatgc atgtgtctca taagatcaat
     1081 ctgagaaata tgattcgaca gcatttcctt ctttgcttca ataacggaag ttatgtcgac
     1141 caggaaatgc ggcggattga gctctgctcc cagcggatcg gacaatgtaa gcggtgagga
     1201 gtgatacagc aggggtgtga cgtcaagcgg tttttcctct accggaacgg gatcaaggga
     1261 agccaccatg gctgctgatt caactatatt ggccgttgtc ctgtgatcag tgtgataatc
     1321 attgggaaga tgagtaaaca cgattccggc tctcaccttg cgcatcaggg aaattgtttt
     1381 cagtctcatt tcccttgtat cgaaaaggaa accgtcaact cccccgaggg tgtagtattc
     1441 cgcatcaagg accgctgcag cctttctggc ttcctcaaac cgtatcctgg ctgtttcctc
     1501 catgctggtg ttgcatcctc cgagatcacc accggtcatg gttgctatta caattttata
     1561 tcccttgtct ttcagaagtt ttaatactcc ggcattccat gcttcagcat catccggatg
     1621 tgcattgatg gaaagcacta ctttgttaaa tttatttgcc ataatatatt atttatttga
     1681 tttttaccat tttcttttcc agtcttgtga attccggaag atcacccacc aggggcttaa
     1741 catattccag gaattcctcc gacacattat ttctttcttc attgaaatag tcttccggca
     1801 tcggtttggc agcaatggcg acctccttaa gcggtgcctt cccgaagcga atcatatatg
     1861 gttcatcaga cattctgtca atgctcacca tgaatccggt ttctcccctg ttggccagtt
     1921 tgactgcttc cacgccgcaa agataggctt cctcaagatc agtctgagag gctcttacaa
     1981 aatcactcat tatcagtgat tcagtaatct ggaattcccc gcggtatccg aatttttcgc
     2041 ttatcatcct gtgaaggttg attcccacac tggtacctcc catggcaccg aactctatat
     2101 tattgaactt atcctttgtt gccgaagcac ttacaggggt tccgtcagca tatttcagac
     2161 cctccccgca gactaccgat acccatccat agtttttgat gcatttctca acatcagtca
     2221 ggaatttatc acggtcgaaa tcaaattccg gagtatagat cagatggggt gcatcatcct
     2281 cttctttgcg tgccagtgca gttgccgcgg caagccatcc cgcatccctg ccgatggtct
     2341 gatatatcac gaatttatcg actctctgca tgtcccttgc aaggattcct gcctgcatca
     2401 cattcaggat gtttgtccgg gcagctgaag cataccctgg tgtatggtcc gttccgaaca
     2461 gatcgttgtc aacggttttc ggaatcccta ttcccctgag ttcatagttc tgatcgcggc
     2521 aataggcctc caccctgttg atggtatcca tggtgtcatt gcccccgatc aggaagaaat
     2581 aacggatatt atacttttta agcaccgaga gtatcctggg cagatcctca tccttcacct
     2641 tgtgccttga agatcccagg gcggaggaag gagtgccccg caatcccttg atcaccgcgg
     2701 gagactgttt gcccaggtca aacatcattt cctgcatgaa gccctcgatg ccataatgca
     2761 tgccatagat ttcgcggatg tttgttgaaa gggagccggc ttcaatgact cctgccaggc
     2821 tggaattgat aactgaagtc ggaccgcctg actgtccgat aattgcattt cctttaagca
     2881 tattcgttat attttacttt atgttttact tgaacttcag cagcccgaaa aattccggcc
     2941 ggtgataatc gggattggga gtatttatcg gattccatgt cacataatgc ggaacagtca
     3001 gcttatcgcc gcatttgtag aaatttgccc tgaaagtctt ccctttaaga tccctgacct
     3061 catgacggaa gaatacctca aagggtatgg ctatggtgat attccatgaa aaattgccgg
     3121 tcctttcgct taccggctgg tctccgaccg aggatttcct cctgatcttt gatattattt
     3181 ccgggtcgac ggttctactg tttgcccttt cggtaccggt tcccagcaat attgtaccta
     3241 tgccgtttac ttcaaaatta tagtatatcc cgtcatctga tggtgataca aagaattcaa
     3301 cgcaggaatc ctcataaaca ccctggtttg accgggtttt ttccgccttg aaatattctt
     3361 cggtgacgta atatttcaga agtatctcgt tatcggtgta ggctattgca aagctgacat
     3421 ccggtttgta actgaaatca ctccagttca gcacatcaac ggcttccttc ttcacattcc
     3481 tgtcaaggct gagggatatt tcatccagat cgggacgggc agtggaaaac ttcagtttct
     3541 caacttcaat tactttcatt tgcatcgggt ttaagttcaa aatcacaaag aacacaatat
     3601 acaacaaaga ttgatacata tttcctccgt atttttaaaa gttatgcaag catttcattc
     3661 catacaagag ctgctgcccc caggacagcg gcattttttt cgggtattcc cgaaggcagt
     3721 attttcactg ttcccctgaa aataggctga acatttttct cgacatattt ccttacgggc
     3781 tcaaaaagca tttcccctgc ctttgccagc cctccgaaca ggaacacggc ttcgggactg
     3841 gaaaatccaa tggcgttatg gattccgaaa gccagttttt cagccgtgaa atccaaagcc
     3901 tttactgcaa ccggatcatt tgcggcagct gcttcggcta tttttttaga agtgagttct
     3961 gatggtttta tatccctgag gatgcttttc tccttcatct cgctcagaag gagaagaacg
     4021 gtccttacca gtccggatgc cgaggcatag gtttcatagc agcctttcct gccgcagccg
     4081 cattcccttc ctcccggaac aattgtaacg tgtccgagtt ctcctgcaaa acccgtatga
     4141 ccgtaaacca tctgcccgtt tatcattata ccgctgccaa gtcctgtgcc aagagtcaga
     4201 acaataaaat ccttcatgtc ctttgctcct ccgaaaatca tttcccccat tgcagcggca
     4261 ttggcatcat tggtgacaat gacgggaaca tctatctttg ccctgatcag atcgcccagg
     4321 ggtactatgc ccttccaggc aagattcggg gcaagttcaa tagtaccctt gtaataattg
     4381 gcattgggag ctcctatccc tattcccagc aatttgaaat cacccgttaa caacaattca
     4441 ttgatcgctg taaccagtgc agaaacaaaa tcattaatct cgggataact gacggtattc
     4501 aggcttcctt cagccacaac cccgccttcc ctgtcaacaa gaccgtatac ggtattggtt
     4561 cctccaatat ccacaccggc tacaatttct ttcatacaac taaaatttaa tattctacat
     4621 tctacccctg gtttttgtcc ctggaggagt ctttctgacg gattttacat tcttttcgct
     4681 tatgtcccac acatattcca gaacccagtt ttcctttatt tcaataatct gcggcatata
     4741 cgaaaccggt tccggctgac gcccggcatg aaaatctccc ggagtccatt tgccatcatt
     4801 gttaagatca taaatcacac gtacctggta aactcctttg tcaagcaggg gaaattcaag
     4861 cctgccgtcc ttgttcataa acacttccct gacaattttt ccctcttcgg tcagaagctg
     4921 gattatcctg cttccttcat ggttgtttat atctagcaca agctttccgt acgactcctc
     4981 accttttacc gtgaatttat aggctgatga atcggaatgt tcgccataaa tattcccgaa
     5041 ggattcggaa tcggctataa aaatatagtt tttattcata ccaagcttac ctttcatgct
     5101 tatcctgcaa gaatttgtac tgtcaatatc caggctgaaa ggtatttttt tccggtcgcc
     5161 tccttccagt tcatacagcc ttattcttga tgtgtccggt ttccggaacg gagtctgtga
     5221 agtaaaaaca agagcctctc ccggttttag ggaggcagta ctgatgtttg tggtaaacct
     5281 gaaaggcgcc ggttttatcc tgcccctggc cggtgtacgg ggaaatatgt atctcatcat
     5341 taccgtatcc tccctgagga catttacacc tgaactgtct gtaaagggat atttaatgac
     5401 cgaagtgatt agcggccggt tgtattgtgc agtgtcggca atccacaccc tgatggagtc
     5461 tccccctttg ttgccctcgg taaaatatga acccggggcg gctccgggta tggaaaaatg
     5521 aaatcccagg ctgtccggcg gaagggaaag agtgtaattg agcctgtaag ctgctcccct
     5581 tgatgaggaa gtgagataac gtatcttctt ttcatgctga aagagataga gtttgtagtc
     5641 tccctgcaga acaattgtat cagctgattt cgttttcccc ggcagctgcc tgagcaggct
     5701 gagggtatcc cggcctggcg gaatccagtt tttttcaggg gtaacagata tagtggaatc
     5761 cagaaaagct atttcctcat cgggcagatt gaaattcttg ctgttatctg catccttcag
     5821 ggcatacagc ctgtattccc gggcagccac attatctatc ctgaaatacc cgttcttgtc
     5881 tgccactgct atatacgacg ggatttccct tatgaaagca gaatcctcct tctggatata
     5941 caggacaacg agtgttgctt caggaggatt gaggtcatcg gctttaagga catttccggt
     6001 aacggagagg gaatcaataa acggtccggt tgaaaacaca aactgaaaat tgtttatggg
     6061 atttccctca ttcagatccc gtaccgcatc ctgaaaatag aaggtatatg ttgtactgtc
     6121 cctcagttct tcttcatacc tgataacaat atcctttccc cttatccgga tctcaggcat
     6181 ccttttcatg ggcggcgaca ccatgaattt ctcatttatc ttgtcaagca ccacgtattc
     6241 atcgaaggaa atgactatcc tgctgcccct gacattcctt gcaccattct ccggaacact
     6301 t
//
LOCUS       DAOSPX010000184         6234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11565_length_6234_cov_4.113934, whole genome shotgun sequence.
ACCESSION   DAOSPX010000184 DAOSPX010000000
VERSION     DAOSPX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11565_length_6234_cov_4.113934"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..1568)
                     /locus_tag="PLZ75_09290"
     CDS             complement(312..1568)
                     /locus_tag="PLZ75_09290"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HOS72625.1"
                     /translation="MKLQRLLFLFISLMVSGLAVGQTYNIVIKGGHVIDPKNNINAVM
                     DVAVKDGKIAMVAKNIDAKEGRQVVDARGFYVTPGLIDMHVHVFQGTVLDHQYSNGPS
                     SVTPDGFTFRSGVTTVVDAGCAGWRSFPDFKRNIIDRSQTRVLALLNIVGDGMRGGPA
                     EQNLKDMEPVRTGEYAKKHSEDIVGIKLAHYSGHEWEPTDLTVEAGRIADMPVMIDFG
                     GAEPPLPIEELFMKHLRPGDIFTHCFAQLGSGREYVVDPVAGKVKPFVWDAIKRGIIF
                     DVGYGGISFAYSQGIPAIKSGLYPHVISTDLHIGSMNAAMKDLVTTMSKFLAMGMDLQ
                     SVIKATTSEPARVIKREELGNLSVGSDADIAILNLRKGKFGLFDYTGHKLESDQKLEC
                     EMTIRGGRIVYDLNGIANPVVLAGRR"
     gene            complement(1635..2585)
                     /gene="hflC"
                     /locus_tag="PLZ75_09295"
     CDS             complement(1635..2585)
                     /gene="hflC"
                     /locus_tag="PLZ75_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOS72626.1"
                     /translation="MKYRKTIIGLILAVIALILILETVYKVREIEQVVITQFGKPVGD
                     AKVTPGLKIKVPFIQRVNYFEKRYMEWDGDPNQVPTKDKKFIYVDTYARWQITDPLQF
                     FKRLTDERGAQSRLADILDGETRDYIANHDLEEVVRSVNRTPVPSGSMGEEVGDTLSD
                     INEGRLRIQEMILKSSNEKASDLGIIILDVRIKRLNYVAEVQTQVYERMKSERYRIAE
                     KFRSEGLGEASKINGEKERDLKTIQSEAYRKAEEIRGEADARATAIYAGAYNQSAASR
                     NLYGFMKSMETFEKTFDTTTTIILSTKSELYKYLINIGQQ"
     gene            complement(2599..3573)
                     /gene="hflK"
                     /locus_tag="PLZ75_09300"
     CDS             complement(2599..3573)
                     /gene="hflK"
                     /locus_tag="PLZ75_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOS72627.1"
                     /translation="MNFYRFNPPIRSGEWKKHLPYYKYLLYGIAALVLLSMTFFQIRP
                     EEAGVITRFGKYIRTESPGLHVKLPVLERLYKVAVERQHKEEFGFRTIYSGIRSDYAK
                     SGTSNESLMLTGDLNLADVEWVVQYRISDPYKYLFKVREPVSTMRDISEACMRQIVGD
                     RTVNEVLTVGRAEIAAIVHQEIQELCTEYSLGLKIEQVILQDVTPPDPVKDAFNAVNQ
                     AQQEKETLINQARSEYNKIIPKARGQAEETIQTATGYAAERVNNALGEAARFNALYRE
                     YIKAPEITKQRLYLETMQKVIPKLGNKIITDEKGNNVLPLLQMQMKNL"
     gene            complement(3588..5051)
                     /locus_tag="PLZ75_09305"
     CDS             complement(3588..5051)
                     /locus_tag="PLZ75_09305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOS72628.1"
                     /translation="MKRSAITLKAGLLFFAVLIISSCAVNPVTGKRQLMLMSEEQEIQ
                     LGFSYDPQVTATFGEYQNDRLLSFIREKTTEMGRISHRPNLQYHIKILDSPVVNAFAV
                     PGGYIYLTRGILAQLNNEAELMGVIGHEMGHITARHSVSQQTKQQLGQLLLIGGMIAS
                     EKFARYAEQAFMGMQLLFLRFSRDNEREADALGVEYSSRISYDAHKMADFFQVLNKMN
                     MAENHGGVPTFLSTHPDPGDRYNSVHRSSAEWQAKLDYPEYKVNQENYLRLIDGIVYG
                     EDPRQGFVENNTFYHPELKFRFNFPGGWRYENTPAQVNMAPADGKALMIFTLASQKTL
                     QNAADTTLAALGLTLLESKNVSVNGLQARATISRQVSQDQSGGQASNLVLSYFIQYSP
                     VIYVFHGVSAEADFNNYFNTMESSMQTFARLTDPSKLNVKPTRLLVKRVPGTATVSAT
                     LSSLGVPQDKLQEIALLNNLELNSTIQAGRLIKVTGQ"
     gene            complement(5160..>6234)
                     /locus_tag="PLZ75_09310"
     CDS             complement(5160..>6234)
                     /locus_tag="PLZ75_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOS72629.1"
                     /translation="GGSKLADNVTPLFERIGFLRESASDLALVLTIIVITYFSIVIGE
                     LVPKVIALNNPEKIAVRIAPVISCFSKFFHAFVRFLTSSTSVIIRILGIKSISEPVTE
                     AELRQMIKLASREGVIEKAQNKIHEKVFYFSDKRAKHIMTHRTEVEWINIRKAEEVLR
                     ADIVKTRHSKVLCCSGTLDNFTGVLFVKDYLIGLSSDKNVKISDFLVQPLIIPETSDA
                     YKVLELFKEKHTQFCVVVNEYGSFEGIITLHDIMENIIGEFPEEGEPFEPDIFIRDDK
                     SVLVNGDAPAEILEGIIEGFTIDYEKVDYSTVAGFVFSKINKIPQIGDKLEYAGYLIE
                     IVDIDGKRIDKVLIREIPDQVRQ"
BASE COUNT         1487 a         1477 c         1337 g         1933 t
ORIGIN      
        1 gagattgcca tatttggtgt gacctgagaa catgggagct ctctggaaac tggtgatatt
       61 atccgcttct ttctcctttg cgacgcttgt tgctgctttg gctgccactc cagctccggc
      121 taaacccaga acagatgcaa acattttctt cagtgcagat cttctttcag ttttcatgtt
      181 ttttagttta atgatttgtg attaatgatt tgtaatttaa tgatttgtaa tttaatgatt
      241 tgtgattgat tgtttgtaaa taataatttt taattagtag cctgtgaatt ataagtttca
      301 gccggcagct gctatctcct gcctgccagt acaaccggat tggcaattcc gttgaggtcg
      361 tatactatcc tgccgcccct gattgtcatt tcgcattcaa gcttctggtc actttccagc
      421 ttatggccgg tatagtcaaa cagcccgaac ttgcccttcc tgaggttcag gattgcaata
      481 tcagcatcac tgcccaccga gaggttgccc agctcctcgc gcttgatcac cctggccggt
      541 tccgaagtgg ttgccttgat cacactctgc aggtccattc ccatagccag gaattttgac
      601 attgttgtaa caagatcctt cattgctgca ttcatgcttc cgatatgcag gtcagtgctt
      661 atcacatggg gatagagacc gctttttatg gcgggtattc cttgtgaata ggcaaagctg
      721 attccaccgt agccgacatc aaatattatg cctctcttta tggcatccca tacgaagggt
      781 ttaaccttgc ctgcaacagg atctacaaca tattcccttc cgcttcccag ctgggcaaag
      841 cagtgggtga agatgtcgcc cggacgaaga tgtttcataa acagctcttc aatgggaaga
      901 ggaggttcag cgccgccgaa atctatcatc acgggcatat cagctattct gccagcttca
      961 acagtaaggt cggtaggctc ccattcgtgc cctgaataat gagcgagctt tatacctaca
     1021 atatcttcag agtgtttttt tgcatattca cctgttctta ccggctccat gtccttcagg
     1081 ttctgttccg cggggccgcc tctcataccg tctcccacaa tattcagcag ggccaggaca
     1141 cgggtctggg acctgtcaat tatatttctt ttgaaatcgg gaaaggatct ccatccggca
     1201 cagccggcat caacaaccgt ggtaacacct gaacggaagg taaatccgtc gggagtcacc
     1261 gaactgggcc cgttcgaata ctgatggtca agaaccgtac cctgaaaaac atgcacatgc
     1321 atgtcaataa gcccgggtgt aacataaaat ccccttgcgt caaccacctg tcttccttcc
     1381 tttgcatcga tatttttggc aaccatagct atcttgccgt cttttacggc aacatccatt
     1441 acggcattga tattattttt agggtcaata acatgtccgc ccttgataac gatattatag
     1501 gtctggccca ccgctaatcc gctgaccatt agactaataa agagaaacaa cagtctttgt
     1561 aatttcattt tcatattatt ctcatttttt ggttaataac tgactcgtaa aatagtaatt
     1621 atatcataaa tatttcactg ctggcctata tttatgaggt atttgtacaa ttcgcttttg
     1681 gttgagagga tgattgtggt tgttgtatca aatgtttttt cgaatgtttc cattgatttc
     1741 ataaagccgt acaggtttct cgaagctgcc gactggttat atgccccggc ataaatggct
     1801 gttgccctgg catctgcctc acccctgatc tcctcggctt tgcggtatgc ttcagactgt
     1861 atggttttaa ggtccctttc cttttccccg tttattttag aagcttcacc aaggccttca
     1921 gatctgaact tttcagcaat tctgtacctc tcgcttttca tccgttcata aacctgtgtc
     1981 tggacttcgg caacatagtt cagtcttttg attctcacat ccagtattat gattccaaga
     2041 tcgctggctt tctcattgga tgacttgagt atcatttcct gtatacgcag gcgtccttca
     2101 tttatgtccg acaatgtatc tcccacttct tctcccattg aaccggaagg aacgggtgtc
     2161 ctgtttacgg atctcaccac ctcttccaga tcgtgatttg ctatatagtc gcgtgtttca
     2221 ccgtcaagaa tatccgcgag ccttgactgt gcaccccttt cgtcggtcag ccgcttgaag
     2281 aactgcagcg gatcggttat ctgccatctg gcataggtat caacatatat gaatttcttg
     2341 tcctttgtgg gtacctgatt gggatctccg tcccattcca tgtatctttt ttcaaaatag
     2401 ttgactctct gaataaaggg tacttttatc ttgagacccg gagtaacttt tgcatcaccc
     2461 accggtttgc cgaactgggt tatgacaacc tgttcgattt ccctgacctt atatacggtt
     2521 tcgagaatca ggatgagtgc aataactgca agaataagtc ctattattgt tttccggtat
     2581 ttcataatat tctgtgattt ataaattttt catctgcatc tggaggaggg gcaggacatt
     2641 atttcccttt tcatcggtaa ttatcttgtt acccagcttt ggtatcacct tctgcattgt
     2701 ttcaagataa agcctctgtt ttgtaatttc cggtgctttg atatattccc ggtaaagggc
     2761 attgaaccgt gcggcttccc cgagggcatt gttgacacgc tcggcggcat atcccgtagc
     2821 tgtctggatt gtttcctctg cctggcccct cgctttcggt ataatcttat tgtattccga
     2881 ccttgcctgg tttatcagtg tttctttctc ctgctgtgcc tggttaaccg cattgaaggc
     2941 atctttaacc ggatccgggg gagtaacatc ctgaaggatc acctgttcaa tttttagtcc
     3001 cagcgaatac tccgtgcata attcctggat ctcctgatgg actattgcag ctatttcggc
     3061 ccgtcccact gtcagtacct cattgactgt ccggtcaccc actatttgtc tcatgcaggc
     3121 ttccgagatg tctctcatgg tagagaccgg ttcccttacc ttgaaaagat atttgtaggg
     3181 atcactgata cggtactgga caacccattc aacatctgcc aggttaagat cccccgtaag
     3241 cataagggat tcattgctgg ttcccgattt tgcataatct gacctgatgc cgctgtatat
     3301 tgtcctgaaa ccaaattctt ccttatgctg tcgttcaaca gcaaccttgt aaagtctttc
     3361 cagcaccggc agcttgacat gaagcccggg tgattcggtg cggatatatt tcccgaacct
     3421 ggtgatcacg ccggcttctt ccgggcggat ctggaaaaag gtcattgaaa gcagcacaag
     3481 agctgcaatg ccatagagaa gatacttgta gtacggaaga tgtttcttcc attccccgga
     3541 tcttattggc ggattgaaac ggtaaaaatt cattttcctg tttttaatta ttgtcctgtt
     3601 actttgatta acctgccggc ctgaatggta ctgttcagtt ccaggttatt cagaagggct
     3661 atttcctgca gtttatcctg aggaacaccg agggagctga gtgttgccga cactgttgct
     3721 gtcccgggta cccttttaac cagcagcctg gttggtttta cattcagttt tgacgggtcc
     3781 gtgagccgtg caaatgtttg catggatgat tccatggtat tgaaataatt attgaaatcc
     3841 gcttcagccg acacaccgtg gaaaacataa ataacggggc tgtactggat aaaatatgac
     3901 aatacaagat tcgaagcctg accgcctgac tggtcctggc ttacctgcct ggaaattgtt
     3961 gccctggcct gtaatccgtt tacgctgaca tttttcgatt caagcaatgt aagcccgaga
     4021 gcagccaggg ttgtgtcagc ggcattctgc agggttttct gtgaagccag ggtaaagatc
     4081 atcagtgctt tgccgtcagc gggtgccata ttgacctggg caggagtatt ttcatacctc
     4141 catccgccgg ggaaattgaa tctgaacttc agttcgggat ggtagaaggt attattctca
     4201 acaaagccct gacggggatc ttcgccatag actatcccgt cgatcagccg gagataattc
     4261 tcctggttca ccttgtattc aggataatca agtttggcct gccattcggc ggatgaccgg
     4321 tgtacggaat tatagcggtc tccgggatcg ggatgggttg agaggaatgt ggggacacca
     4381 ccgtgatttt ccgccatatt cattttgttt agcacctgaa aaaaatctgc cattttatga
     4441 gcatcatagc ttatcctgga agagtattcc actccaagtg catcagcttc ccgttcattg
     4501 tcacggctga atctgaggaa aaggagctgc atccccataa aagcctgttc cgcataccgg
     4561 gcaaacttct ctgaagctat cattccgcct atcagcagca gctggcccag ttgctgcttg
     4621 gtctgctggc ttaccgagtg gcgtgctgta atatgtccca tttcgtgacc aataacaccc
     4681 ataagttcag cttcattgtt aagctgtgcc agtatgcccc gtgtgagata tatatatccg
     4741 cccggaacgg caaatgcatt taccaccggt gaatccagta tttttatatg atactggaga
     4801 ttgggcctgt gggagatcct gcccatctcc gtggttttct ccctgataaa actgagtaac
     4861 ctgtcattct gatactcacc gaaggttgca gttacctgcg gatcatagga aaaacccagc
     4921 tggatttcct gttcttctga catcagcatt agctgtctct tgcctgttac cgggtttacg
     4981 gcacaggaag agatgatcag aactgcaaaa aacaataagc cggcttttag tgtaatagct
     5041 gatcttttca tattgattta aatttatgat gtattcaaat cagtttgaag gagtttaaga
     5101 tatcagtttt caaatatcgt gaaaaacttc atttgtttgc ctgttttgcc caatgtttct
     5161 cattgccgga cctgatccgg tatttcccgg atcagaacct tgtctatcct cttaccgtct
     5221 atgtccacta tctcaatcag ataacctgca tactccagtt tatcacctat ctggggaatt
     5281 ttattgattt tgctgaaaac aaatccggca acggtcgagt agtcaacctt ttcgtaatcg
     5341 atggtaaatc cttcaattat gccttcaagt atttcggcgg gagcatcacc atttacaaga
     5401 acagacttgt catcccttat aaaaatatca ggttcaaaag gctcgccttc ctctgggaat
     5461 tccccgataa tattttccat tatatcgtga agggtgataa ttccttcaaa ggagccgtat
     5521 tcattaacca caacacagaa ctgggtatgc ttttccttga aaagctcaag taccttatat
     5581 gcatcagatg tttccggaat tataaggggc tgaacaagga aatcactgat ttttacgttt
     5641 ttatcagaac tgaggccaat caggtagtct tttacaaaga gtactccggt gaaattgtcg
     5701 agtgtgccgc tgcagcacaa aaccttgctg tggcgggttt tcactatatc agccctgagt
     5761 acttcctctg ccttcctgat attaatccat tccacctcgg tacggtgagt cattatgtgc
     5821 tttgccctct tgtcggaaaa gtagaagacc ttttcatgga ttttgttctg ggccttttca
     5881 atgacaccct ccctggaagc gagctttatc atctgtctta attccgcttc agtgaccggc
     5941 tcacttattg attttatacc cagtatcctg attatgacag atgttgatga agtaaggaat
     6001 cttacgaaag catgaaagaa tttgctgaag caggaaatca ccggagcaat tctgacagca
     6061 atcttttcag ggttgttcag ggcaatgact tttggaacaa gttctccgat gacaatggaa
     6121 aaataggtta ttacaatgat tgtcagcaca agtgccagat cacttgcgga ttccctgagg
     6181 aacccgatcc gttcaaacag cggggtgacg ttgtccgcaa gcttactgcc tccg
//
LOCUS       DAOSPX010000185         6219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11595_length_6219_cov_4.454250, whole genome shotgun sequence.
ACCESSION   DAOSPX010000185 DAOSPX010000000
VERSION     DAOSPX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11595_length_6219_cov_4.454250"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1947)
                     /locus_tag="PLZ75_09315"
     CDS             complement(<1..1947)
                     /locus_tag="PLZ75_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOS72630.1"
                     /translation="MTYIKFEKSRIVNLEYSLSREIIRTNRAGSYISTTIAECNTRKY
                     HGLLVCPVDELGGGRYLLLSSLDVTVAYQDKSFNIGLRKYKGDYFNPRGHKYLEELET
                     EIIPVRVYRVGNVLLKMERLFVHYEEQVLVRYTVLEATEPMKLQIRPLLAFRSIHELT
                     HANLAANTKIDWIKNGIRSRMYEGFPYLNLQFSTKADFVHMPDWYLGVEYPEEQKRGY
                     DYSEDLFTPGFFEIQASKGDSIVFSASTKEEDPAGFAQKFTDTVAGKIPRNSFLNCLR
                     NAAQQFVEKRGEDVLIIAGYPWFGAWGRDTFISLPGLALARHKLKLYEMVFDTQIRRM
                     KDGLFPNMGTGGHYAFNSIDAPLWFFWALQQYVLSGGKDCCNKYSNAVKSVLNAYRYG
                     TEFNIRMREDGLIYSDAPGKALTWMDAVVHDGPVTPRSGYAVEINALWYNAVCFALEM
                     AKVENDRKFISEWKHLPELIKESFLELFWDEKAGYLADYVNDEEGRNMFVRPNMVIAV
                     SLPYSMLTKEQMKKVLDVADRELVTPRGLRTLSPANRYYQGIYSGNQEERDRAYHQGT
                     VWPWLFGPFCEGWLKVYGKQGINKVRKLIYGLEEVMDEAGITTLSEIHDGDPPHAPRG
                     TISQAWSVGEVLRIIDLIEEYSKNN"
     gene            2127..3290
                     /locus_tag="PLZ75_09320"
     CDS             2127..3290
                     /locus_tag="PLZ75_09320"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOS72631.1"
                     /translation="MIRNSLIFLFILLPLSACKNNNIEIIGKLTNPIKDEYIYLDELR
                     SSGLVTIDSSLIAEDGAFSFSFRGKVPAFYLLKTDETNFLTMLLEPGQVLEINASHDS
                     LNFPESLKGSKGTELMAGYNRKLSETVGKLSGLSEIYQQSVGTPELPLVMEQLDSIAR
                     IYLEDINSYTKKYIDENLSSLVALTALYQQVAPGEYVLNPQRDLEYFIKVDSSLYSLY
                     PGYEPVKTLHEQVMALVSDMTAQNIIDSRFRYGTEAPEISLPDPDGNTAKLSSTRGNI
                     VLLDFWAAWCPPCRKENPNLVRAYDLYHNRGFTIFQVSLDRTKEAWTKGISEDNLGRW
                     IHVSDLKYWNSAVIPVYNIEQIPTNYLLDREGRIIASNLRGQELLNRLEQIFN"
     gene            3323..4486
                     /locus_tag="PLZ75_09325"
     CDS             3323..4486
                     /locus_tag="PLZ75_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOS72632.1"
                     /translation="MKKYFFLIIPLLVLASCTRQNHFTVKGSFRALARKTIYMSRVKV
                     NETIPVDSAKTDARGLFRFRVKAGETDFYQLGTSPDNFITLLAGPGEKIFLSSDEENF
                     AENYTVEGSEGSEEVRLLDIRLLKTKKGLDSISGLYRKAETEADFETRGKALEEEYVK
                     LLKDQRRFNIEFILGNMRSLSSIKALYQKVDDDTYVLYESRDLQYMKIVSDSLQKYYP
                     RSNHTRALVADLSREMNQFYARQLEQISSSLPETRLDPDLKDLTGKRIRLSSLKGKYV
                     LLTFWSVESRDCIAENLQLKEFYKTYHRKGFEIYQVNLDRDEEAWRNAVRFDELPWIS
                     TREDDPSNPQIARLYNVRSLPANYLYDPEGTIVARDIHGRTLQLKLSQVFNTR"
     gene            4483..5238
                     /locus_tag="PLZ75_09330"
     CDS             4483..5238
                     /locus_tag="PLZ75_09330"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOS72633.1"
                     /translation="MNELKNPGKIYFASDFHLGLKTGTPPLERERKVVRWLNDISSDA
                     SEIYLAGDVFDFWWEYRLVVPRGFTRFLGCISSLTDRGIAVHFLTGNHDMWVRDYISS
                     ECGVIIHTSPVTAEFNGRRFHIAHGEGLGSDSLAYRILLKIFRNKVLRALYSSLHPSI
                     GVGIGHWWSLSSRLGKGIRLEFMGEENEDLIKYARSLAQKEKIDYFIFGHRHLAMTFR
                     LNNGSEICFLGDWINQGSFAEWDGNRLTLKTPD"
     gene            complement(5319..>6219)
                     /locus_tag="PLZ75_09335"
     CDS             complement(5319..>6219)
                     /locus_tag="PLZ75_09335"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOS72634.1"
                     /translation="ESSGMSMILYSKIRGGYTVTTGNVISSENVEDLRRRLEEINRLD
                     PDEIRSVYRRMLTDSSLSSKGGAGLGLIEMAKKTGNRLDYDFRRLNDRQHYFMLSKTV
                     DSEGMGFHTGSAEKQFSGDKALKLGRMMARNNVYLIWSGHLNQAVGREVISFTEKKLS
                     EENIEQTLRKRVFSILVEMIENVAKYSPGREDEEKYGMPLAMLRFRQGRYHISTGNLV
                     RNSQTEILREKMNIISRLDNDELREYFKKSLSEQTPETESTGNMGLIEMAWKSGNKLH
                     YRLSPVNDIYSYFIINARIDSRI"
BASE COUNT         1646 a         1499 c         1381 g         1693 t
ORIGIN      
        1 attatttttt gaatattcct caataaggtc aattattctc aggacttcac ccacactcca
       61 tgcctgggag atcgtacccc ggggtgcatg gggcggatca ccgtcatgta tctcggaaag
      121 agtggtgatt ccggcttcat ccattacctc ttcaagcccg tagataagtt tcctgacctt
      181 atttattccc tgttttccgt atactttcag ccagccttca cagaaagggc caaagagcca
      241 cggccataca gttccctggt gataggccct gtccctttct tcctggtttc ctgaataaat
      301 cccctgatag taccggttcg ccggagaaag tgtcctgagg cccctgggcg ttacgagctc
      361 ccggtcggca acatccagta cttttttcat ctgttccttt gtgagcatgc tgtagggaag
      421 ggaaaccgca atgaccatat tcggccgtac aaacatgttt ctgccttcct cgtcgtttac
      481 atagtcagca agataacctg ccttttcatc ccagaacaat tcgaggaatg attccttgat
      541 cagttccgga agatgtttcc attctgaaat gaatttcctg tcattttcca ctttcgccat
      601 ttcaagggca aagcatacag cgttatacca gagtgcattt atttccacgg cataaccgct
      661 tctcggcgta accggtccgt catgtacaac agcatccatc catgtaaggg ctttccccgg
      721 ggcatcggaa tatatcagtc cgtcttccct catccggata ttgaattctg ttccgtacct
      781 gtaggcattc agtactgatt tgacagcatt actgtatttg ttgcagcaat cctttccgcc
      841 gctgagtaca tactgctgaa gcgcccagaa aaaccacagt ggcgcatcaa tggaattaaa
      901 ggcataatgc cctcctgttc ccatgttagg aaacaggccg tccttcatcc tcctgatctg
      961 ggtatcgaaa accatttcgt acagtttcag cttatgccgt gcaagtgcaa gtccgggcag
     1021 ggatatgaag gtatcccggc cccaggctcc gaaccaggga taaccggcaa tgatgaggac
     1081 atcctcccct cttttctcaa caaactgctg ggctgcattc ctcaggcagt tcaggaaact
     1141 gttgcgcggt atcttgccgg caaccgtatc agtgaacttt tgggcaaatc ctgccgggtc
     1201 ctcctccttt gtggaggctg aaaagacaat ggaatcaccc ttgctggcct ggatttcaaa
     1261 aaacccgggg gtaaagaggt cctcggaata atcatagcca cgcttctgtt cctcaggata
     1321 ttcaaccccg aggtaccagt cgggcatatg aacaaaatcc gccttcgtcg aaaactgcag
     1381 gttcagatat gggaaaccct catacatcct ggatctgatt ccgttcttta tccagtcaat
     1441 cttggtgttg gccgcaagat ttgcatgagt aagctcatgt atgctcctga atgccagtaa
     1501 aggtcttatc tgaagcttca tgggttcggt ggcttcaaga acggtatacc ttaccagcac
     1561 ctgctcctcg taatgaacaa aaagcctttc catcttgaga agaacattcc ccacacggta
     1621 tactctgacg ggaataattt cagtttcaag ttcttccagg tatttatgac ctctgggatt
     1681 aaaatagtca cccttgtatt ttctcagtcc gatgttaaag cttttatcct gatatgccac
     1741 ggttacatcc agtgaagaca gcaaaaggta cctcccgcct cccagctcat caacgggaca
     1801 aaccagcaga ccgtgatatt tacgcgtgtt gcattcggct atcgttgtcg agatatacga
     1861 acccgccctg ttggtcctta ttatttccct cgaaagagaa tactccagat tcactatccg
     1921 gctcttttca aacttgatgt aagtcatatg attttggttt tgtcgggtat aaaaacttct
     1981 ataatttaaa aatattttgt taataattca atactgaaaa tgagggagta atcaattata
     2041 tcagttattg tcaggtttac ggaagaaaaa aagaataact ttgcatttca aaattgttaa
     2101 atgattatca attaatgttt tgttcaatga tcaggaattc tttgattttt cttttcattc
     2161 tgctgcctct ttcagcttgc aaaaataata atattgaaat aattggcaaa ctcacaaatc
     2221 caattaagga tgaatatatt tatcttgatg agctaaggtc ttccggcctt gtaaccatcg
     2281 attcttcact gattgcggaa gacggggcct tttcattcag tttcagaggt aaagtaccgg
     2341 cattttacct cctgaagacg gatgagacca actttctgac catgctgctt gaacccggcc
     2401 aggttctgga gatcaatgcc agtcacgact cgctgaattt cccggaatca ctgaaggggt
     2461 caaagggtac tgaactcatg gccggataca acaggaaact cagcgaaacc gtgggtaaac
     2521 tgtccggact gagtgaaata tatcagcaga gcgtggggac gcctgagctg ccgcttgtaa
     2581 tggaacaact ggacagcatc gcccggatat acctggagga tatcaattcc tacactaaga
     2641 aatacatcga cgaaaacctt tcctcgctgg tcgcgcttac cgccctgtat cagcaggttg
     2701 ccccgggtga atatgtcctt aatccccaga gggacctgga atatttcata aaggttgatt
     2761 catcgctgta ttccctgtat cccggctatg aacccgtgaa gacgcttcat gagcaggtaa
     2821 tggcccttgt atccgacatg accgcacaga atatcattga ttcccggttc aggtacggga
     2881 ctgaagcccc tgaaataagc cttcccgatc ctgacggcaa tacggcaaaa ctctcatcta
     2941 caaggggaaa tattgtatta ctggatttct gggctgcctg gtgccctccc tgcagaaagg
     3001 aaaatccaaa cctggtaagg gcatatgatc tttatcataa caggggattt actattttcc
     3061 aggtttcact tgaccggaca aaagaggcat ggacaaaggg cataagcgaa gataacctgg
     3121 gcaggtggat ccacgtcagc gacctgaaat actggaattc ggccgtgata cccgtctaca
     3181 acattgaaca gatacccaca aattacctgc tcgacaggga gggcaggatt attgcgtcaa
     3241 acctgagagg tcaggaactt ctgaacaggc ttgaacagat attcaactga attattatcc
     3301 tttaaaaagt aattgctgaa tcatgaagaa atattttttc ctgatcattc cgcttctggt
     3361 cctcgcatcc tgcaccaggc aaaatcattt taccgtgaag ggatcattca gggcccttgc
     3421 ccgtaaaaca atttatatga gcagggtgaa ggtgaatgag accataccgg tcgattcggc
     3481 aaagaccgat gccaggggcc tgttcagatt cagggtaaag gccggggaaa cggattttta
     3541 ccagctcggg acttctccgg ataattttat cacccttctt gccggacccg gtgaaaaaat
     3601 cttcctcagc tcggatgagg aaaattttgc agagaattac acagtggagg ggtctgaagg
     3661 ttcagaagag gtaaggcttc tggatatcag gcttctgaaa acgaaaaagg gcctggattc
     3721 aatttcaggg ctttacagga aggcagaaac agaagctgat tttgagacaa ggggaaaagc
     3781 tcttgaagaa gaatatgtaa aacttctgaa agatcagaga agattcaaca ttgaattcat
     3841 ccttgggaat atgagatccc tgtcatccat caaggccctg taccagaagg ttgatgatga
     3901 tacatatgtt ctttatgaga gccgcgacct gcagtacatg aaaatcgtgt cggattccct
     3961 tcagaaatat tatccgcggt caaatcatac cagggcactg gtagccgatc tgtcacgtga
     4021 aatgaaccag ttctatgccc ggcagcttga gcagatatca tcctccctgc cggaaacaag
     4081 gctggatccc gatctgaagg atcttaccgg taaaagaatc agactgtcct ccctgaaggg
     4141 gaaatatgta ctgctaacct tctggtcggt tgaatcgcgg gattgcattg cagaaaacct
     4201 tcagctcaag gaattctaca aaacctatca caggaaagga tttgagatat accaggtgaa
     4261 tcttgaccgg gatgaggaag cctggagaaa tgctgtcagg tttgatgaac tgccatggat
     4321 cagcaccagg gaagatgatc cgtcaaatcc gcagattgca aggctttaca atgtccgttc
     4381 actgcctgcc aattatctct acgacccgga aggaacaata gtggcaaggg atattcacgg
     4441 caggacactt cagctgaaac tcagtcaggt attcaacacc aggtgaacga attgaagaat
     4501 ccgggcaaaa tatacttcgc atccgacttt catcttggcc ttaaaaccgg cactcctccc
     4561 cttgagcgtg aaaggaaggt tgtcaggtgg ctgaatgaca tatcttccga tgcatcggaa
     4621 atttacctgg ccggtgatgt tttcgatttc tggtgggaat acaggcttgt tgtgcctcgt
     4681 ggattcacca gattcctagg atgtatctca tcactgaccg acagggggat agcggttcat
     4741 ttccttacgg gcaaccacga tatgtgggtg agggattata tctcctctga atgcggggtt
     4801 atcatacaca cttccccggt aaccgccgaa ttcaacggca ggcgttttca tattgcccac
     4861 ggagaagggc tcggatcaga cagtctggca tacaggatac tgctgaaaat attccgtaac
     4921 aaagttctca gggcactcta ttcatcactg cacccctcaa taggtgtagg catcggacac
     4981 tggtggtcgc tcagttccag gctgggaaaa gggataagac tggaattcat gggtgaagaa
     5041 aatgaagacc tgattaaata tgccagatcc ctggcccaga aggaaaagat agattatttc
     5101 attttcggac accgtcacct ggcaatgact ttcagactga ataacggttc agaaatctgc
     5161 tttctcggcg actggatcaa tcagggcagt tttgccgaat gggacggaaa cagactgact
     5221 ctgaaaacac cggattaggc agaacattaa tatccgggac cgcttcagta ttatacggtg
     5281 ccgaaatctg ctttccgcaa cactgaatca gcaggatact aaatccggct gtctattctg
     5341 gcgttaatta tgaaatagga atagatatca ttgaccggtg atagccggta atgtagtttg
     5401 tttcctgatt tccaggccat ttcaatcaac cccatattac ctgtgctctc agtttccgga
     5461 gtctgttccg acaatgattt tttgaaatac tccctgagtt cgtcattatc cagtcgggaa
     5521 atgatattca tcttttccct gagaatttcc gtctggctgt tcctgacaag atttcccgtt
     5581 gagatatgat atctcccctg tctgaatctc agcattgcca gtggcattcc gtacttttcc
     5641 tcatcttcgc ggcccggact gtattttgca acattctcta tcatttccac aagaatgctg
     5701 aatactcttt ttctcagtgt ctgttcaata ttttcctcac tgagcttctt ctcggtaaag
     5761 gagataacct ccctgcccac agcctggttg aggtgtcccg accagatcag gtaaacattg
     5821 ttccgggcca tcattctgcc cagcttcagg gctttgtccc cgctgaactg tttttcagca
     5881 cttcccgtat gaaaccccat tccttcagaa tccaccgtct tgctgagcat gaaataatgc
     5941 tgcctgtcat tcagcctgcg aaaatcataa tccagcctgt tgcccgtttt ttttgccatt
     6001 tcaatgagtc ccagtcctgc tcctcccttg ctgctcagtg atgaatcagt cagcatccgg
     6061 cggtagacgg atcttatttc atcagggtcc agcctgttta tctcctcaag gcgccttctg
     6121 agatcttcaa cattttctga ggaaattaca ttgccggtgg tgaccgtata ccctcccctg
     6181 atctttgaat acaaaatcat cgacatacct gagctttca
//
LOCUS       DAOSPX010000186         6199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11631_length_6199_cov_4.782715, whole genome shotgun sequence.
ACCESSION   DAOSPX010000186 DAOSPX010000000
VERSION     DAOSPX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11631_length_6199_cov_4.782715"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLZ75_09340"
     CDS             <1..204
                     /locus_tag="PLZ75_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HOS72635.1"
                     /translation="EAIATIMAIQNDTVPPTINFRVPDPQIDQKLNITPNKAQARKIN
                     AAISNTFGFGGHNASIVIKKADT"
     gene            210..944
                     /gene="rnc"
                     /locus_tag="PLZ75_09345"
     CDS             210..944
                     /gene="rnc"
                     /locus_tag="PLZ75_09345"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOS72636.1"
                     /translation="MFPGNSNGSCRHDRKEFRSLLKKILGFRPGNIGIYEAAFVHRSS
                     SYTLPDGRIINNERLEYLGDAVLDTILSDYLFRVFPEASEGFLTKTRSRIVNREVLGR
                     IALSIGIDKLLISNVNSVRNSKNLCGDAFEALIGSVFLDKGYNKTRKMFISRILKKNP
                     GLINIISTDTDYKSLVFEWVQKNKAGLRFTYEEDYDTDQKKPVFTSTLLINDEESGKG
                     QGATKKEAEQEASRNAWKKITGNFRT"
     gene            969..1415
                     /locus_tag="PLZ75_09350"
     CDS             969..1415
                     /locus_tag="PLZ75_09350"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HOS72637.1"
                     /translation="MKEKPRIRRVRLKNSNIHEWTLFGIVSSEPDYRLSLALNKKLDI
                     SLKSCNPLVIHDSHGIEMSFSKFSSSANPAEMNCDLVSNRSLESFLLRKMKNIDYFLI
                     IHDSPEEKESSALLKKLRETEGITAAIKIEAGSLKGKDLENIIRQV"
     gene            1749..3305
                     /locus_tag="PLZ75_09355"
     CDS             1749..3305
                     /locus_tag="PLZ75_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS72638.1"
                     /translation="MKRSTIALSATFFFLTLSGLILIQLYWIRNAIEITDQEFRMLAN
                     KALESVILDLEENELIRRIAEEIDPASSDSVTAIVPANSPLARKLRGYQPNLKLLEMY
                     GLADPGEPVAITGAGQKIFISDENIPEYTPDELIESSSQIYNAEFTGRVSNKIVILET
                     IMDKILYSTPDIRERINPEDLKPMLRSALSNVGINLDFEYSIRSGLYGDLWQTPGFNS
                     KPGTNKFIIQLFPNDPVPGQNQLILYCLQEQQYKFRRIGLLGIMSILFTLTLLILSTG
                     TFIIIFRQKKLSEIRNDFISNMTHELKTPISTISLASQLMADKTIPDIKKNIDSLADV
                     IFDESMKLKFQVERVLQMAIFEKARMKFNLIPGDINKVVRNAMDNFTLQIESRNGKLI
                     DNLDAALPVAMIDEIHLMNAISNLIDNAIKYSREAPFITVSTRNTRKGIVIVLEDKGI
                     GISKEGLKRIFDKFYRVPSGNVHNVKGFGLGLSYVKKVVEEHNGIIKAESHPNKGTKF
                     SILIPQNDGK"
     gene            3302..3988
                     /locus_tag="PLZ75_09360"
     CDS             3302..3988
                     /locus_tag="PLZ75_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007902828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS72639.1"
                     /translation="MKNTRILLAEDDSNLGNLLKNYLTVKNYETELFVNGIEALKAFM
                     KEHYDIFILDIMMPEMDGLTLAREIRKTRPEAPVIFLTAKSQKEDIIEGFKTGADDYI
                     TKPFSMEELLYRIQAILKRTASLNNSRKQEFYEIGKYTFDTVKQLLVFGDEVINLTTK
                     ESELLELLCRYGKDILERNFALRSIWIDDNYFNARSMDVYITRLRKYLKKDPEVKILN
                     VHGKGYKLIS"
     gene            complement(4160..5947)
                     /gene="lepA"
                     /locus_tag="PLZ75_09365"
     CDS             complement(4160..5947)
                     /gene="lepA"
                     /locus_tag="PLZ75_09365"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOS72640.1"
                     /translation="MDNIRNFCIIAHIDHGKSTLADRLLERTNTIAERDFHDQVLDSM
                     DLERERGITIKSHAIQMEYESDGRKYFLNLIDTPGHVDFSYEVSRSIAACEGALLVID
                     ATQGIQAQTISNLYMAIENNLEIIPVLNKMDMASAMPDEVKDQIVDLTGCKREAIIEA
                     SGKTGMGIDLILREIVQTIPPPKGDPGAPLQALIFDSIFNSFRGIIVYFKIVNGTLHR
                     GDRVKFVSTGHEYNAEEIGVLRNRQEAREIMRTGDVGYLISGIKISSEVKVGDTITHV
                     KNPCREAISGFEEVKPMVFAGIYPVDADDYEDLRSSMEKLQLNDASLTFVPESSAALG
                     FGFRCGFLGLLHMEIVQERLDREFDMDVITTVPNVSFRVITTKGEEIEVHNPSGLPPV
                     TGIDRIEEPYIVAQVITHADYTGSIMNLCIDKRGTLKNQVYLTSDRVELTFEMPLSEI
                     VFDFYDKLKSISKGYASFDYYYTEYQKADLVRLDILLNGEMVDALSTLIYRGHAYDFG
                     RKMCVKLKELIPRQQFEIAIQAAIGAKIIARETVKAVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQIGNVEVPQQAFLAVLKLD"
BASE COUNT         1908 a         1408 c         1272 g         1611 t
ORIGIN      
        1 gaagcaattg caacaataat ggccattcag aatgataccg tacctcccac aatcaatttc
       61 agggttccgg atccccagat tgaccagaaa ttaaatatca ctcccaacaa ggcccaggca
      121 agaaaaataa acgccgcaat cagcaatacc ttcggtttcg ggggtcataa tgcctcaatc
      181 gtaataaaaa aagcagatac ttaaaatccg tgttccccgg gaattcaaac ggcagctgtc
      241 ggcatgacag aaaagaattc agatcccttc ttaaaaaaat tcttggtttc agaccgggaa
      301 atatcggaat atacgaagca gcatttgttc accgctcctc atcctatacc ctgcccgacg
      361 gaaggatcat aaacaatgaa agacttgaat acctcggcga tgccgtcctt gataccatat
      421 tgtcggatta cctgttcagg gtgtttccgg aggccagtga agggtttctg accaaaaccc
      481 gttcccggat tgtcaacagg gaggtgcttg gccggatcgc tctttcaata ggaatagaca
      541 agctcctcat aagcaatgtg aattcggtaa ggaattcaaa gaacctttgc ggtgatgcct
      601 ttgaagccct gatcggatcc gtattcctcg acaagggata caataaaaca aggaagatgt
      661 ttatcagcag gatacttaaa aagaaccccg gcctgatcaa tataataagt accgacactg
      721 attacaagag cctggtcttt gaatgggtgc agaaaaacaa ggcaggactc aggttcacgt
      781 atgaagaaga ctatgatact gatcagaaaa agccggtctt cacatctact cttctgataa
      841 acgacgaaga atccggaaag ggacagggag ccacaaaaaa ggaagccgaa caggaagctt
      901 cccgcaatgc atggaaaaaa attaccggga atttcagaac ctagtatcat atcaaaggag
      961 gaaacacaat gaaggaaaaa ccgaggatca ggcgcgtcag actcaaaaac agcaatattc
     1021 atgaatggac ccttttcggt attgtttctt ccgaaccgga ttacaggctg tcactggcac
     1081 tcaacaaaaa actggatatc tcacttaaaa gctgcaaccc cctggtaatt cacgacagcc
     1141 atggtattga aatgagtttc agtaagtttt ccagttctgc caatccggca gagatgaact
     1201 gtgaccttgt gtcaaaccgc tcgcttgaat cctttctttt gagaaaaatg aaaaacattg
     1261 actatttcct gattatacat gacagtcctg aagaaaaaga aagcagtgcc cttctgaaaa
     1321 agctccgtga gacggaaggc atcactgcag caataaaaat agaagccgga agcctgaagg
     1381 ggaaggatct tgaaaacatt atacgccagg tctgaataaa taaagaagag ccgcttccgg
     1441 gcggaagcgg ctcacttttt gataacccta aaactaacct aacctatgaa aaaaacctct
     1501 atatctttta aaacaaattt catgccaaat tatcattgca aagatacggc aggaatctta
     1561 attttacaaa cgtttccaca ggatagcatg aaacattaac aaaaatttaa taaaatttaa
     1621 aatatcctga tcctcctttt tcaggcgaag agtctgatca gacataatat acacattcct
     1681 tccccctgtg ttaaagaaag ttaaattctt agggcttaat ctggatatag tagtactttt
     1741 gtaactgtat gaaaagaagc acgattgcat tatcagccac tttctttttc ctgaccctgt
     1801 caggtctgat actgattcag ctctactgga tcagaaatgc aattgaaata acggatcagg
     1861 aattcaggat gctcgccaac aaggccctgg aatccgttat acttgacctg gaggaaaatg
     1921 agcttataag gaggatagcg gaagaaattg atcctgcatc atccgattct gtaactgcaa
     1981 tcgttccggc aaactcgcct cttgccagga agctgagagg ataccagccc aatctgaaat
     2041 tactggaaat gtacggactc gctgatcctg gagagcctgt ggcaataaca ggtgccggac
     2101 agaaaatatt catttccgat gaaaacatac cggaatatac cccggatgaa ctgattgaaa
     2161 gctcatccca gatatacaat gccgaattta ccggacgggt atcaaataaa attgtgatcc
     2221 tcgaaaccat aatggataaa atcctttaca gcacgcctga catcagggag aggataaatc
     2281 ctgaggatct gaaacccatg ctgcggagtg ccctgtcaaa cgtgggaata aacctggatt
     2341 ttgaatattc gatacgctcg ggactgtacg gagatctctg gcagaccccg ggttttaatt
     2401 ccaagccggg aacaaacaaa ttcattattc agcttttccc caacgatccg gttccgggtc
     2461 agaaccagct gattctctat tgcctgcagg agcagcagta taaattcagg agaataggat
     2521 tgcttggaat catgtcaatt ctgttcaccc tgacactgct gatactctca acaggtacct
     2581 ttataataat attccggcag aaaaaactgt cggagatacg gaatgacttc ataagcaata
     2641 tgacacatga actgaagacc ccgatttcaa ccatatccct ggcatcccag ctgatggccg
     2701 acaaaaccat tccggacata aaaaagaata ttgattcact cgcggatgtg attttcgacg
     2761 aaagcatgaa actcaaattc caggttgaaa gggtacttca gatggctatc tttgaaaaag
     2821 ccagaatgaa attcaatctg attcccgggg atataaacaa agtggtcaga aatgccatgg
     2881 ataatttcac acttcagatt gaaagcagga acggaaagct cattgataat ctggatgcag
     2941 cacttcctgt agccatgata gacgagattc atctcatgaa tgcaatatca aatctgatag
     3001 acaatgctat caaatattca agagaagctc cttttattac cgtttcaacc cgcaatacca
     3061 ggaaaggcat tgtaattgtt ctggaagata aaggaatagg aatctccaag gaaggactca
     3121 aaagaatttt tgacaaattt taccgtgtgc cttccggcaa tgtacataac gttaaaggat
     3181 tcgggctcgg actgagctat gttaaaaagg tggttgaaga gcacaacggg ataatcaagg
     3241 cagaaagcca ccccaacaaa ggaacaaaat tctcaatttt aattcctcaa aacgacggaa
     3301 aatgaaaaat acaaggattc ttctggcaga agatgacagc aatctgggaa atctgttaaa
     3361 aaactatctg actgtcaaga attatgaaac ggaacttttc gtaaacggaa tagaagccct
     3421 gaaggccttc atgaaagagc attacgatat tttcatcctt gatattatga tgcctgaaat
     3481 ggacggactc accctggccc gtgagatcag gaaaaccagg ccggaggctc cggttatttt
     3541 ccttacggca aaaagtcaga aggaggatat cattgaaggc ttcaaaacag gagctgatga
     3601 ttatattaca aaaccattct caatggaaga actcctttac aggatccagg ctatcctgaa
     3661 acgaacggct tccctcaata attcaaggaa acaggaattc tatgagatcg gaaaatatac
     3721 attcgacacc gtaaaacaac tgcttgtctt cggcgacgag gttataaacc ttaccacaaa
     3781 agaatcagaa ctccttgaac ttctgtgcag atacggcaag gacattctgg aacgcaattt
     3841 tgcgctacgg tcaatctgga tagacgacaa ctactttaac gccagaagta tggatgtcta
     3901 tatcaccagg ctaaggaaat acctgaagaa ggatcccgag gtgaagattc tcaatgtaca
     3961 cggaaagggt tataagctta tttcctaaaa aattaccctg accaaaattg atcagggtat
     4021 tcggttcagg ttagataatc ggattaatag tttttctatc caattcccat caggaggcta
     4081 aattaacatt ttttttatat ctggtgaaaa ttccctgaaa aaaagcaaat aataatggat
     4141 ttatccgttt catcagggct cagtcgagtt taagaacggc aagaaatgcc tgctgaggaa
     4201 cttccacatt acctatctgt ctcattcttt ttttcccttt cttttgtttc tcaagaagtt
     4261 tcctctttct tgttatgtca cccccgtaac attttgcagt aacatccttt ctgacggctt
     4321 ttaccgtttc cctggcaatt atcttggccc ctatcgctgc ctgaatggca atctcgaact
     4381 gctgcctcgg tataagttct ttcagcttca cacacatttt tcttccgaaa tcatatgcat
     4441 gtcccctgta aataagagtt gaaagcgcat ccaccatctc accgttgaga agtatgtcga
     4501 gcctgacaag atcggctttc tgatattccg tataatagta atcgaatgaa gcatatcctt
     4561 ttgaaatgct tttaagcttg tcataaaagt cgaagacaat ctctgacagg ggcatttcaa
     4621 atgtcagttc aaccctgtca cttgtcagat acacctggtt tttcagtgtg ccccgcttgt
     4681 caatgcacag attcattatt gaaccggtat aatccgcatg ggttataacc tgtgcaacta
     4741 tatacggctc ttcaatacgg tcaatgcctg taacaggagg aagacccgat ggattgtgaa
     4801 cctcaatctc ctcacccttt gtcgtgataa ccctgaagga tacgtttgga acggttgtga
     4861 tcacatccat gtcaaactcg cggtcgagtc tttcctgaac tatttccata tgaagcagtc
     4921 cgaggaagcc gcatctgaat ccgaatccca gggcagcaga tgattccggc acaaatgtaa
     4981 gggaagcatc attgagctgc agtttctcca tcgaacttct cagatcctca taatcatcag
     5041 catcaacagg gtatatcccg gcaaaaacca tcggcttcac ttcctcaaag cctgaaattg
     5101 cctccctgca gggattcttc acatgagtga ttgtatcccc gaccttgacc tcggaggaga
     5161 tcttgatgcc ggaaatgaga tagccgacgt cgccggttct cattatctcc cttgcttcct
     5221 gcctgtttct caggactccg atttcctcgg cattgtattc atggccggta ctgacaaact
     5281 taaccctgtc tcccctgtgc agggttccgt ttacgatctt gaaatatacg attattcccc
     5341 tgaaggaatt gaagatggaa tcaaagatca gagcctgcag gggcgccccg ggatcaccct
     5401 tgggcggagg aatcgtttga actatttccc ggagtatcag atcaataccc attccggtct
     5461 tcccgcttgc ctctattatc gcctcccttt tacatcctgt caggtcaact atctgatcct
     5521 tcacctcatc aggcattgcg ctggccatat ccatcttgtt cagaacaggt ataatctcaa
     5581 gattattttc aatggccata tagaggtttg aaatggtctg agcctgaatt ccctgggttg
     5641 catcaatgac aagaagtgct ccttcacagg ctgctatgga acgtgaaacc tcatacgaaa
     5701 aatccacatg cccgggcgtg tctatcaggt tgagaaaata tttcctgcca tccgattcat
     5761 attccatctg gatggcatgg ctcttgattg tgatccccct ctcccgttcc agatccatgc
     5821 tgtcaagaac ctgatcatga aaatcccttt cggctatcgt attggtcctt tcaagaagcc
     5881 ggtcggccag ggtgcttttg ccatggtcta tatgggctat aatgcagaag ttacgaatat
     5941 tgtccatcag ggcagaggct aataaaattg ctgcaaatat aacacaataa ataaaaaaga
     6001 gaggctgtcc cttcttgctt tgagacagcc tctgcagtaa ataattatat atgcagtttt
     6061 acatcattcc cggcattccg ccacccattc cctgcggagg cattggcggg gtttcttcct
     6121 tttcctcaac aataacagct tcggttgtaa ggaacattcc ggcaattgat gccgcatttt
     6181 caagagccac acgggtaac
//
LOCUS       DAOSPX010000187         6159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11715_length_6159_cov_3.586501, whole genome shotgun sequence.
ACCESSION   DAOSPX010000187 DAOSPX010000000
VERSION     DAOSPX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11715_length_6159_cov_3.586501"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLZ75_09370"
     CDS             <1..516
                     /locus_tag="PLZ75_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOS72641.1"
                     /translation="PERVATPDLADDILAMANRERMSIFLFGAQENILSECRNNIEKK
                     FPKIVIAGSRNGYYRPEEEKDIFDLINKTNPDILFLGMSSPKKELFFESYRHKLNARY
                     ILGVGGYFDIISGHIRRAPRWMQDTGLEWLFRLIQEPRRLWKRYLIGIFQFFCLVTKE
                     KFRRVFKRKGE"
     gene            517..930
                     /locus_tag="PLZ75_09375"
     CDS             517..930
                     /locus_tag="PLZ75_09375"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOS72642.1"
                     /translation="MVIDHISFAVKNLEEGISYWSRVFGYRQLTNIVINSLQKVKVVF
                     LDKEDSIPVKLIEPVEGNLSLQNFVNRGGGFHHICFKCSDVNEKLNELSDKGLLVLVP
                     PQPGEAFNNHEIAFLLARYGLNIELIDTEERHGLL"
     gene            1000..2052
                     /locus_tag="PLZ75_09380"
     CDS             1000..2052
                     /locus_tag="PLZ75_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOS72643.1"
                     /translation="MSNNSDCCQVIVDPRARIVYASYYIQGIVDLFGAQKVCFRISPF
                     KLLVKKYDNLAFDEFFAFIIIRNGQRIKIVIDYKDNYTINYEIYDWCDVYGKVNLNPK
                     YTDLLDYKKIINIGPGFGIRIMGNFATILNAMSNFIKSYRYSDIEYYPFFYGYYWQTK
                     RLPISDFTHSPTDGKYVFFVSTLWNHDNCIRSTNILRAAFIEECKNAGLNFEGGLYAR
                     PSNREYNKYKHLVYSKFISFPSYISMTKKSSVVFNTPTVFNCHGWKLGEYFALGKAII
                     STPLINKMPVDLEHGKNIHFIEDASEMKDAIDKILSDHKYRATLEEGALGYYNKYLSP
                     EIVIKRILQHPACNPW"
     gene            complement(2061..2606)
                     /locus_tag="PLZ75_09385"
     CDS             complement(2061..2606)
                     /locus_tag="PLZ75_09385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOS72644.1"
                     /translation="MTLRYIIDRLTLAFIAKASSNTRIRYWRRQGMKIGDNCFINNVN
                     FSTEPYLIEIGNHVAIAAGTDFITHDGAIWCFRDEIKNADIFGKIQIGNNVFIGNNCT
                     ILPNTVIGDNCIVGAGSIVRGHFPENSVIVGNPAKTVMPVSMQKLFYMQNPNLFTIKN
                     LGFKEKKRIIKSHFDLIPRTD"
     gene            complement(2637..3362)
                     /locus_tag="PLZ75_09390"
     CDS             complement(2637..3362)
                     /locus_tag="PLZ75_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72645.1"
                     /translation="MKNAIFLHHSTGYSIWLGKTNNYIYKITNKGDVQKFFERYNNEN
                     KTTYQIKEKFYPKGEPYGWKNYPFDYYNIWVKNAGKEPYMEEATLETLTGEYDLIIFK
                     HCFPVSRIQPDSGTPDIDSEIKSLENYKLQYDALKEKMHQFPDNKFIVWTPAVNTQKL
                     MTEDEAIRTREFRDWVINDWDIKGDNIFVWDFYSYETEGGLYLAEKNAVSPDNSHPNA
                     GFAKRVAPLFGQFIIDVFESRVD"
     gene            complement(3567..4844)
                     /locus_tag="PLZ75_09395"
     CDS             complement(3567..4844)
                     /locus_tag="PLZ75_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOS72646.1"
                     /translation="MKKSLSFFVIVCFFATLGLFQGCKKSTIPELTTVEISDVGLTSA
                     VSGGTIITDGGEEITAKGVCWSTSTNPTIADSKTSDGKGSANFTSNIVGLSEGTTYYV
                     RAYATNEVGTAYGNELSFTTNAVTSAVLTTTEVSNVTSTSAVAGGNITDDGGGNITAR
                     GVCWGTSPNPTVNKTTDGTGKGTFTSNITGLSDGTVYYYRAYATNSSGTSYGEEFSFI
                     TPVNDIDGNVYQTVKIGNQVWMAKNLLTTKYNDDSAIPNVIDNTEWSALKTGAYCWAN
                     NDETTYKPLYGALYNWYAVETGKLCPTGWHVPSDNDYKTLEMSLGMSQDQANGTGLRG
                     TDEGSKLKSNNGWSLDGNGSNSSGFNALPAGYRYYGDGTTKGLGLITYFWTSDAIDDL
                     LATFRRLDAEETEVSRDGTHKEAGKSVRCIKNQ"
     gene            complement(5046..>6159)
                     /locus_tag="PLZ75_09400"
     CDS             complement(5046..>6159)
                     /locus_tag="PLZ75_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOS72647.1"
                     /translation="MSSCAKDPTLPVLTTDEAIEITTNSATISGNVTEDGGAEITARG
                     ICWGTVTMPTLSDNFKPSGTGPGKFSCVISELEPNTEYYVRAFAENRVGIAYGNEVTF
                     KTGIATPSVTTGQVTDITLNSAVCAGTVINSVGAPVIEKGICWSQAPDPDLDDSHASV
                     SAGTDTYTCTLTGLSSGSRYYARAYAKNEGGTAYGEQVVFSTKVLDIEGNIYGTVCIG
                     NQVWMKENLRTTKYNDNSPIPIVLSDTEWKTIVTPAYCWFNHNEGYKNTFGALYNWYT
                     VETGKLCPAGWHVPDDNEYKTMEISLGMSVEQAELWEWRGTDEGYKMKSTTTWNNNGN
                     GSNSSGFNGLWRISFRANWCILRAGNSDLLVEFRSR"
BASE COUNT         1743 a         1271 c         1192 g         1953 t
ORIGIN      
        1 cctgaaaggg tagcaactcc cgatcttgca gatgatatac ttgctatggc aaatcgcgaa
       61 cggatgagta ttttcctgtt tggtgcgcag gagaatatcc tttcggagtg caggaataat
      121 attgaaaaga aatttccaaa aatcgtaatc gccggaagtc gtaatggtta ttacaggcct
      181 gaagaggaga aagacatatt cgaccttata aacaagacta accctgacat tctgtttctc
      241 gggatgtcct caccaaagaa ggagctcttt ttcgaaagct acaggcataa actgaatgca
      301 aggtatatac ttggtgtagg gggttatttc gatatcatat caggtcatat caggcgagcc
      361 ccgcgatgga tgcaggatac cggccttgaa tggttgttca gactcatcca ggaaccaagg
      421 aggctttgga aaagatacct gataggaata ttccagttct tctgcctggt aacaaaggaa
      481 aaattcagga gagtttttaa acgtaaagga gaatagatgg ttattgatca catttcattc
      541 gctgtaaaga atctggaaga gggcatcagc tactggagcc gtgttttcgg gtacagacag
      601 ttgaccaaca tagtgatcaa ttctcttcaa aaggtaaaag ttgtatttct tgataaagaa
      661 gatagtatcc ctgtgaaatt gattgaaccg gtagagggga atctgtcgtt gcaaaacttt
      721 gtgaacaggg gcggaggctt tcatcacata tgtttcaaat gttcggatgt aaatgaaaaa
      781 cttaacgagt tatctgacaa aggtctattg gtgttagtcc caccccagcc cggggaagca
      841 tttaataacc atgaaatagc atttttactt gcacggtacg gacttaatat tgaattaatt
      901 gacacagaag aaagacatgg attattatag atccgaaaaa aatactacaa caaggttaaa
      961 ccagtatatc aaacgattat aaatatttca taaaacaagg tgagtaataa cagtgactgc
     1021 tgccaggtga ttgttgaccc acgggcaaga atagtctatg cttcatatta cattcagggg
     1081 attgttgatt tgtttggagc gcagaaggtt tgtttccgga tctccccttt taaactgttg
     1141 gtgaagaaat atgacaatct tgcctttgat gaattctttg catttattat tatcagaaat
     1201 ggacaaagaa taaagattgt tattgactat aaggataact atactataaa ctatgagatt
     1261 tacgactggt gtgatgtata tgggaaggtc aatctgaacc ccaaatatac tgacttgctc
     1321 gattataaaa agataataaa tattggaccg ggatttggta tcaggatcat gggaaatttt
     1381 gcgacaatcc ttaatgccat gtctaatttt atcaaatcat acaggtattc agatatagaa
     1441 tattatcctt ttttctatgg ttattattgg cagacaaaaa gacttccaat atcagacttt
     1501 actcatagtc cgactgatgg caaatatgtt ttttttgtat caacactctg gaatcatgat
     1561 aattgcatta ggagcaccaa tattcttcga gctgctttta tagaagaatg taaaaatgcg
     1621 ggattaaact ttgaaggagg tttatatgcc aggccatcaa atagggaata taataaatat
     1681 aaacatctgg tatattcaaa gtttatttct ttcccatcgt acatcagtat gactaaaaag
     1741 tcttcagttg ttttcaatac tcctacagta ttcaattgtc atggatggaa actgggtgaa
     1801 tattttgcat tgggtaaagc cataatttcg acacccctga taaacaaaat gccggttgat
     1861 ctggaacacg gaaagaatat acacttcatt gaggatgcat cagaaatgaa agatgcgatt
     1921 gataagattc taagcgatca caagtatcgg gcaactcttg aagaaggagc ccttggatac
     1981 tacaataaat atctgagtcc tgaaattgta ataaaaagaa tactacaaca tccagcctgc
     2041 aatccctggt agtgttgaaa tcagtccgtc cgaggtataa ggtcaaaatg actttttata
     2101 attcttttct tttctttaaa tccaagattc ttaatggtaa atagattagg attctgcatg
     2161 taaaacagct tttgcatact taccggcatt actgttttcg cgggattgcc aacaataacc
     2221 gagttttccg ggaaatgccc gcgtactata cttcccgcgc ctacaatgca attatcacca
     2281 atcacagtat taggtagaat tgtacaatta ttgccgataa aaacgttatt gccgatttgt
     2341 attttgccga aaatatcggc attttttatt tcatctctga agcaccagat agcgccatca
     2401 tgggttatga agtcagtccc ggctgcaatg gcaacatgat ttccgatctc aataagatat
     2461 ggttctgttg aaaaattcac attattgata aaacaattat caccaatctt catcccctgt
     2521 ctgcgccagt atctgattct tgtattactg ctggctttgg caatgaaggc taatgtgagc
     2581 cgatcgatta tataccttag agtcatcgtt ttgaggtagt attggaaaaa aacgggttaa
     2641 tcaactcttg attcaaatac atcaataata aattgtccga aaagaggagc cacacgtttt
     2701 gcaaatccgg cattcggatg cgaattatca ggtgagacag cattcttttc tgccaggtaa
     2761 agtcctccct ctgtttcata ggaatagaaa tcccatacaa atatattatc tccttttatg
     2821 tcccaatcat ttattaccca gtcacgaaac tcacgtgtcc ggatggcttc atcttcggtc
     2881 atgagctttt gggtattgac agcaggcgtc cagacaataa acttgttgtc aggaaactga
     2941 tgcatttttt ctttaagtgc atcatattgc agcttataat tctcaaggct tttaatctcc
     3001 gaatcaatat ctggtgtacc tgaatctggt tgaattctac tgacagggaa gcaatgcttg
     3061 aatattatca ggtcatattc cccggtcaga gtttccagtg tggcttcttc catgtaaggt
     3121 tcttttcctg cattttttac ccagatattg taataatcaa aggggtagtt tttccagcca
     3181 tatggttccc cctttggata aaacttctcc tttatttgat aggttgtttt attctcattg
     3241 ttatatctct caaaaaactt ttgaacgtca cctttgtttg tgatcttgta aatgtaattg
     3301 tttgttttgc ctaaccagat tgagtagcct gtactgtgat gaagaaatat tgcatttttc
     3361 ataggtgttt catttttttc gcatcccgta aaaagaataa ctgttaatat caataatact
     3421 tttttcatga ccttttttca aagatataca attaaccttc atccattgtt ttcataagct
     3481 tgtggtggca gctcttccct attataatat aacaatgagg ctatcctttt gagatagcct
     3541 catttataat atcacatgtt atgccattat tggttcttta tacaccgcac agattttccc
     3601 gcttctttat gtgttccatc ccgggaaact tcagtttctt ctgcatcaag ccttctgaag
     3661 gtggccagga ggtcatctat agcatcagat gtccagaagt aggtaatcag gcctaatcct
     3721 ttggttgttc catctccata gtatctgtaa cctgccggca gcgcattaaa cccactggaa
     3781 tttgaaccgt ttccatcaag cgaccagcca ttgttacttt ttaatttact gccttcgtca
     3841 gttcctctca aacctgttcc attagcctga tcctgagaca ttcccagact catttccaat
     3901 gttttatagt cattatctga cggtacatgc cagccagtgg ggcatagttt gcctgtttcc
     3961 accgcatacc agttataaag agctccgtac aatggtttat aggttgtctc atcattattc
     4021 gcccaacaat acgcgccggt ttttagtgca gaccattccg tgttatcaat aacattcgga
     4081 atagccgagt catcattgta ttttgttgtg agcaggttct tagccatcca gacctggttg
     4141 ccgatcttga cagtttggta cacgttcccg tcaatatcgt ttacaggtgt gataaagctg
     4201 aattcctcac cataagaggt gcctgaactg ttggttgcat aagcacggta atagtaaaca
     4261 gttccgtctg acagtccggt tatattactg gtgaatgttc ccttgcctgt accgtcagtg
     4321 gttttattga ctgtgggatt tggagaggtg ccccagcaga ctcctctggc agttatattg
     4381 cccccgccat catcggtaat gtttccaccc gcgacagccg atgttgatgt cacatttgat
     4441 acttcggtgg ttgtcagcac ggcacttgtt accgcattag tggtaaagga aagttcatta
     4501 ccataagccg ttcccacttc gttagtggcg tatgcacgta catagtaggt tgtcccttcc
     4561 gatagtccaa caatattgct ggtaaaattg gctgaacctt taccatcact tgtcttagaa
     4621 tcagcaatag ttggattggt tgaggtactc cagcacacac cttttgctgt gatctcttcg
     4681 cctccgtcag tgataatggt tccaccggaa acggctgatg tgaggccaac atcacttatt
     4741 tccacagtgg tcagttccgg gattgttgat tttttacatc cctggaacaa ccccaatgtc
     4801 gcaaaaaagc atactattac aaaaaaactt aaactttttt tcatgttttt gtatttacag
     4861 tttaacttat ttcttatgat ttatactggt aatttgcaaa tatgttgccc ccaatgataa
     4921 aaaacaggcc atatcagtct ttaatacacc ttatatattt accacctctt tttgatgtaa
     4981 ctgacctgtg gactccacta ttatcaaaat caagccttcg gtaaacagcc tgcgcattat
     5041 catttttatc tggatctgaa ctccaccaat aagtcagaat tcccagcgcg tagaatgcac
     5101 cagtttgccc gaaacgatat cctccatagc ccgttaaacc cacttgaatt actgccatta
     5161 ccattattgt tccaggttgt tgtgctcttc atcttataac cttcatcagt tcccctccat
     5221 tcccacaact ctgcctgttc aacggacatt cccaacgaaa tttccattgt tttatattca
     5281 ttgtcatcag gtacatgcca tccggcagga cacagctttc cagtttcaac agtgtaccaa
     5341 ttgtaaagag caccgaaagt attcttatag ccttcattgt gattgaacca gcaatatgcc
     5401 ggtgttacaa ttgttttcca ctcagtatca cttaaaacaa tgggtatggg agaattgtcg
     5461 ttatattttg ttgtcctcag gttttctttc atccatacct gatttccaat gcaaacagta
     5521 ccataaatat tgccttcaat atctaaaacc tttgtgctga agacaacctg ctcaccgtaa
     5581 gctgttcctc cttcgttttt cgcataagct cttgcataat acctgctccc tgatgaaagg
     5641 ccggttagtg tgcaggtata agtgtccgtt ccggcgctga cagaagcatg agaatcatcc
     5701 aggtcaggat caggtgcctg actccagcat ataccctttt ctatgacagg ggcacctacg
     5761 ctattaatta ctgtaccggc acatacggca ctgttgagtg ttatgtccgt cacctgccct
     5821 gtagttactg atggcgttgc aattcctgtt ttgaatgtca cctcgttgcc ataagctata
     5881 ccaactctgt tttcagcaaa agccctgaca taatactcag tattcggttc cagttcgctt
     5941 atgacacagg aaaattttcc gggacccgtg cctgatggtt taaaattatc agaaagtgta
     6001 ggcatggtaa cggtgcccca gcaaatacca cgtgcagtta tttctgctcc tccgtcttct
     6061 gttacattgc cgcttatagt ggcagaattg gtggtgatct caatcgcttc atccgtggtt
     6121 aacaccggaa gggtagggtc ttttgcacaa ctgctcata
//
LOCUS       DAOSPX010000188         6144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11745_length_6144_cov_3.828379, whole genome shotgun sequence.
ACCESSION   DAOSPX010000188 DAOSPX010000000
VERSION     DAOSPX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11745_length_6144_cov_3.828379"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..2253)
                     /locus_tag="PLZ75_09405"
     CDS             complement(139..2253)
                     /locus_tag="PLZ75_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOS72648.1"
                     /translation="MALRDDIVFRGGILYFALAIVALAILIRIVILQTVEYGKWSAMS
                     EKYVFKRTELVADRGDILACDGRQLATSVPYYTIYMDTRSSGMPEETWAAGINGLSAG
                     LARHLGTRTAPEWKAALNNARRRGDRYFLIKRKVDYKTLKNLRELPVFREGRYRGGLI
                     AEAENRRILPNNELAARTIGYLSQGEKGNTVGIEGSFDRELSGRNGAAVMQRLAGGDW
                     ITVDGTGTVSSRDGNDVVTTIDIDLQDVAATALANQLRKHNAHHGCAVVMKVNTGDIR
                     AMVNLEAGSDGRYHESYNYAVGESTEPGSTFKLPVLMAALEEGIIDTGDIVDTGRGSI
                     RFYDKVIRDTKEGGYGMISVREVFEKSSNVGTARLITDLYGDRPGDFVDRLYAMKLNQ
                     KLGIRIQGEGTPLIRYPGDKLWSGISLAMMSYGYEVRMTPLQILTFYNAVANNGKMVR
                     PRFVTAIERNGREIKHYNPEVIIKSVASRSTIKKVRKMMEGVVEHGTATNLKNTDYKI
                     AGKTGTAQIAKGSGGYRSDSGISYQASFVGYFPAEDPLYSCIVVVNAPSNGVYYGNQV
                     AGTVFREIADRIYATSFYRDYRSSDGNRTAALVPQAGNGYRDDIHSVLGRFGGRPEKT
                     TGHEWIKTRQNGDTVSCTGIEITGGMVPDVRGMSLRDAMHLLENSGYRVRFSGRGRVL
                     RQFPEHGTRYFEGQVVSLEMNL"
     gene            complement(2259..2612)
                     /locus_tag="PLZ75_09410"
     CDS             complement(2259..2612)
                     /locus_tag="PLZ75_09410"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOS72649.1"
                     /translation="MEGEGKDKSRKISTGSFLKELISGGIPSRIILENIWYVMLLTLL
                     GAVYIANRFHAERITRDMQGLRTEVRELRAQSLSTSAELMFISRQSEVYRMVRERGLD
                     LEELKTPAFKLIVDN"
     gene            complement(2616..3518)
                     /gene="rsmH"
                     /locus_tag="PLZ75_09415"
     CDS             complement(2616..3518)
                     /gene="rsmH"
                     /locus_tag="PLZ75_09415"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOS72650.1"
                     /translation="MKVYHIPAMPEQSIEGLNIRSGGIYVDVTFGGGGHSREILRRLD
                     KGRLIAFDQDDDAVANLPSDRRFTFLNQNFRFLRNNLRFLGIEAIDGLIADLGVSFHQ
                     FDEAERGFSFRFDAPLDMRMNREGSRTAADLIRTSDEADLADMFFDYGELKNSRIIAR
                     EIVKARKEKALVRTGDLLNVLDFLAPPGQKHKFYAKVFQALRIAVNHEMENLREMLLQ
                     AREMLKSQGRLVVITYHSLEDRLVKNFMRSGNFEGYGKKDFYGNPEPAPFRVLTRRGI
                     TPDKDEIAANSRVRSARLRIAEKI"
     gene            complement(3518..3979)
                     /locus_tag="PLZ75_09420"
     CDS             complement(3518..3979)
                     /locus_tag="PLZ75_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOS72651.1"
                     /translation="MATFIGENFCKVDAKGRITLPAAFKKQMPSDARDHFIVRKDIFD
                     NCLVLYSLDDWNKQLEKIRKKVNPYNRAHSSFLRNFFKGMAELELDSNSRFLVPRRLL
                     DLIDAGRDVVLAGQEGRIEIWAAENYHKTDISGEELASLAEDILGGNSNEE"
     gene            4204..5064
                     /locus_tag="PLZ75_09425"
     CDS             4204..5064
                     /locus_tag="PLZ75_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOS72652.1"
                     /translation="MNESNETGPVLQIDVKKVLASKNPSLARILPSFLIRYLKNIIHQ
                     DEINSFLLRYGHLRDAEFVSAILESMGTGYRVYGHENIPRSGRYIFVSNHPLGGLDGL
                     VFMNELSKYYSGIKFPVNDLLLNLTNLSGIFLPVNKHGTQARDAIKKLEEAYASDSQM
                     LYFPAGLCSRKKHGKITDLKWHKSFISKAIQHKRDIVPAFFSGRNSDFFYNLANIRTF
                     LGIRANIEMLYLPDEMFRQKGRDLALVFGRTIPWQTFDASKTQEEWAGWVKSKSYELK
                     SMISGEKPDK"
     gene            5116..6078
                     /locus_tag="PLZ75_09430"
     CDS             5116..6078
                     /locus_tag="PLZ75_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS72653.1"
                     /translation="MEAIVERQPLDQIIAELTKERFLRKTNNANNEVYIFDYNESPVL
                     MHEVGRVRELTFRHAGGGTGNSIDIDKYDTAAEDPQKQLIVWDPEHRKVIGGYRFYFP
                     PKGCVKCDITKLASSEYFNFSRKFLTRFYPYVMELGRSFVHPEYQSAARGRKSLFALD
                     NLWDGLGALIIENNHIKYLFGKVTMYPHFNQNARNMILYFLKKHFRDPDRLVTPIMPA
                     EIDIDMEKMKKLFTRRRYRDDYKILSAEVRKLGETIPPLINAYMNLSPTMRTFGTFIN
                     HSFGEVEETGIMITLRDIYVEKINRHLASYKKDFMITWFSPDSQ"
BASE COUNT         1557 a         1636 c         1275 g         1676 t
ORIGIN      
        1 agtcccctta ccgcaacaaa aagggaatcc ggatccgctt tccgggaatc gaacacgata
       61 ttgctgatca ttctttcctt ccggccggaa aaagacttta tatcaatacc cttcaatatt
      121 ttttcaagtt ctttcatatc aaagattcat ttcaagtgac actacctgtc cttcaaaata
      181 cctggtcccg tgttcaggga attgtctcag caccctgcct ctgccgctga agcggaccct
      241 gtaaccggaa ttctcaagca gatgcattgc atctctcagg ctcatacccc tcacatccgg
      301 aaccatcccc ccggttattt caatccccgt gcagctgact gtatccccat tctgccgggt
      361 ttttatccat tcatgcccgg tggtcttttc cggcctgcca ccaaaccttc ccagaacact
      421 gtgaatatca tcacggtatc cgttacctgc ctggggaaca agcgctgctg tcctgttccc
      481 gtcagatgac ctgtagtctc tgtaaaaact agttgcataa atcctgtctg ctatttccct
      541 gaataccgta cctgccacct gattgccgta atagactccg tttgaggggg catttacgac
      601 aacaatgcag gaatacagag gatcttccgc ggggaaataa ccgacaaagg aagcctggta
      661 ggaaataccg gaatccgatc tgtagcctcc gctccctttc gcaatctggg ccgttccggt
      721 tttgcctgcg attttgtagt cggtgttttt cagattcgtt gccgtaccgt gttcaacaac
      781 cccttccatc attttcctga ctttttttat ggtggacctg gatgcaactg atttgattat
      841 gacttcggga ttatagtgtt ttatctccct gccgttcctc tcgattgcgg ttacgaaacg
      901 cggtctgacc atcttcccgt tattggccac tgcattgtaa aatgtaagta tctgcagggg
      961 tgtcatccgg acttcatagc cgtatgacat catcgccaga ctgattcctg accacagctt
     1021 gtcgcccgga taccttatga gaggtgttcc ttcaccctgt atccttatgc ccagcttctg
     1081 attgagtttc atggcataga gcctgtcgac gaaatccccg gggcggtcac cgtacagatc
     1141 tgtaatcagc ctggctgtcc ccacattgga tgatttctcg aatacctccc tcacgcttat
     1201 catgccgtat ccgccttcct tggtatcccg tatcacctta tcgtagaacc tgatgcttcc
     1261 ccttcccgtg tccacaatat caccagtatc aattattccc tcttcaaggg cggccatcag
     1321 aaccggcagc ttgaacgttg atcccggttc ggtgctttcc cctactgcat agttgtagct
     1381 ttcatgatac ctgccgtcac ttcctgcctc gagattgacc attgccctta tatcgcccgt
     1441 attcactttc atcaccacgg cacatccgtg atgtgcattg tgctttctga gctgatttgc
     1501 cagtgctgtt gcagccacat cctggagatc aatatctatg gtggtcacca catcgttgcc
     1561 gtcgcgtgaa ctgaccgtgc ctgtgccatc caccgtaatc cagtcccctc ccgcgaggcg
     1621 ctgcatgact gccgctccgt tcctgccgga aagttccctg tcaaaggaac cctcaatacc
     1681 aacagtattg cctttctcgc cctggctcag atagcctatg gttcttgcag ccagttcatt
     1741 gttaggcagt attctcctgt tttcagcctc agctatcagt ccgcccctgt agcggccttc
     1801 acggaaaacc ggaagttccc tcaggttttt aagtgtttta tagtcaacct tcctttttat
     1861 aagaaaatag cggtcgccgc gcctgcgggc attatttaat gctgccttcc actccggggc
     1921 cgttctggtt cccagatgcc ttgccagacc ggcagaaagg ccattgatgc cggctgccca
     1981 ggtctcctcc ggcattccgc tgctacgcgt atccatgtag atggtataat agggaaccga
     2041 ggtggccagc tgccttccgt cacaggcaag aatatccccc ctgtcagcaa caagctcggt
     2101 ccttttgaaa acatattttt cactcatggc cgaccatttg ccatactcaa ccgtctgaag
     2161 tatcacaatg cgtattagaa tggccagggc aacaattgcc agtgcaaaat ataaaatgcc
     2221 gccacggaat actatgtcat ctctgagagc cataatcgct aattgtcaac tatcagttta
     2281 aatgccggtg tcttcagttc ttccaggtca agcccccttt cccttaccat ccggtaaacc
     2341 tcggattgcc ggctgatgaa catcagttca gctgaagtgg aaagagactg ggctctcagt
     2401 tctcttactt ctgtccgcag gccctgcatg tcgcgtgtga tcctttctgc atgaaacctg
     2461 tttgctatat acactgctcc gagcagagtc agcagcatga cataccagat attttccagg
     2521 attatccttg acgggattcc tccgcttata agctccttca ggaaactgcc ggtgcttatc
     2581 tttctgcttt tatcctttcc ttccccttcc ataaatcaaa tcttctcggc aatgcgaagt
     2641 cttgcactcc ggaccctgct gttcgcggca atctcatcct tatccggggt aattcccctt
     2701 ctggtcagca ccctgaaggg agccggctcg ggattgccgt aaaaatcctt cttcccgtag
     2761 ccttcaaaat tgcctgaacg catgaaattt tttaccagcc tgtcttccag ggaatgatat
     2821 gttataacca caagtcttcc ctggctttta agcatctccc gggcctggag aagcatttcc
     2881 ctgagattct ccatctcgtg gttgactgct attctcaaag cctgaaacac tttcgcgtaa
     2941 aacttgtgct tctgcccggg cggggccaga aagtcaagca cattaagcag atctccggtc
     3001 cgaaccaggg ctttctcttt ccttgccttc acaatttccc ttgctataat cctcgaattt
     3061 ttcagctccc cgtaatcaaa aaacatatca gccaggtcag cttcatccga tgttctgata
     3121 aggtcagccg ccgtccggct tccttccctg ttcatcctca tatcaagagg cgcatcaaac
     3181 ctgaaggaaa atccccgttc agcctcgtca aactggtgaa aggacacccc cagatcagca
     3241 atcagaccgt ctatggcttc tatccccagg aacctgagat tgttcctcag gaatctgaaa
     3301 ttctggttaa ggaatgtaaa gcgcctgtcg gacggaagat tggcaacggc atcatcatcc
     3361 tggtcgaatg caatgagcct ccccttgtca agccgcctga gaatctccct ggaatgcccc
     3421 ccgcccccga aagtgacatc cacataaata ccgccggaac ggatattaag cccttcaata
     3481 ctctgttccg gcattgcagg tatatgatag accttcatca ctcttcatta ctgttaccac
     3541 ccaatatgtc ctcggcaaga cttgcaagtt cctctccgga aatatcagtc ttgtgataat
     3601 tctctgcagc ccatatctct attctgcctt cctgacctgc caggacaaca tcccttccgg
     3661 catcaatcag atcaagcagg cgcctcggaa caaggaagcg gctgttgctg tcaagttcga
     3721 gttcagccat gcccttgaaa aaattacgta ggaaactgct gtgtgcccga ttatagggat
     3781 tgactttctt ccttatcttt tccagctgtt tgttccagtc atccagcgaa taaagtacaa
     3841 gacagttgtc aaaaatatcc ttgcggacaa taaaatgatc cctggcatca gaaggcatct
     3901 gctttttaaa cgccgcagga agtgttatcc tccccttggc atctacctta caaaaatttt
     3961 caccgataaa ggtggccatg acttactata atctcaatcc ggatgctaaa ataaagaaaa
     4021 tttctccact ttgtcccact ttgtcccact tttttaacgg gctttgttga taacttttcc
     4081 caaaacggtt gattgcggcc gcaaatgagg ctctctttaa tcacaaaaag ttattgacaa
     4141 cacgggctct cagctgtaaa aaattgtatt tttacatgat cactacccgg aatcactatc
     4201 actatgaacg aatcaaatga aacagggcct gtcctgcaga tagacgtgaa aaaggttctg
     4261 gcatcgaaaa atccttcact ggcaagaatc ctgccttcct ttctgatcag ataccttaaa
     4321 aatatcattc accaggatga gattaacagt ttcctcctca ggtacggtca tctcagggat
     4381 gcagaattcg tatcggccat acttgaaagc atgggcacgg gatacagggt ttacggccat
     4441 gaaaacattc cccgctcagg cagatatata tttgtttcca atcatcccct gggagggctt
     4501 gacggactcg ttttcatgaa tgagctttcg aaatactaca gcggcataaa atttcccgta
     4561 aatgatcttc tgcttaatct tacaaatctt tccggcattt tcctgccggt caacaagcat
     4621 ggcacacagg ccagggatgc gataaaaaag cttgaggagg cctatgcatc cgactctcag
     4681 atgctgtatt ttcctgcagg actatgttcc aggaagaagc acggaaaaat aacagatctt
     4741 aaatggcata aaagttttat ttcaaaagca atacagcata aaagagatat tgttccggca
     4801 tttttctccg gcaggaactc ggattttttc tacaaccttg ccaatatcag gacattcctg
     4861 ggcatcaggg caaatattga aatgctatat cttcctgatg agatgttcag acagaagggc
     4921 agggatctgg cacttgtttt cggcaggacg atcccatggc agacatttga tgcttcaaaa
     4981 acccaggaag aatgggccgg atgggtaaaa tcaaagtcat atgagctgaa atcaatgatt
     5041 tccggagaga aaccggataa ataatttcta aattgcaccc tttttacacc ctatcccgaa
     5101 tcaatacaac acaaaatgga agcaattgtt gaaagacagc cactggatca gataattgca
     5161 gaacttacaa aggaaaggtt cctccggaaa accaataatg caaataatga ggtttacatt
     5221 tttgactata atgaatcacc cgttttgatg cacgaagtgg gccgggtcag ggaactcacc
     5281 ttccgccatg cggggggcgg cacaggcaat tcaatcgata ttgacaaata cgatacggca
     5341 gcggaggatc cccagaaaca gcttattgta tgggatccgg aacacaggaa agtaatcggg
     5401 ggctacaggt tttattttcc tcccaaaggc tgtgtgaaat gtgatattac aaaactcgcc
     5461 tcatcagaat attttaactt ttccaggaaa ttccttacaa ggttctatcc ctatgttatg
     5521 gaactcggac gatcattcgt ccatcccgag tatcagtcag cggcaagggg aagaaaaagc
     5581 ctgtttgcac tggataacct gtgggacggc ctgggtgcac tgataattga gaacaatcat
     5641 attaaatatc tcttcggaaa ggtgacaatg tatccccact ttaatcagaa tgcaaggaat
     5701 atgattctat acttcctgaa aaagcatttc agggatcccg acaggcttgt gaccccgatt
     5761 atgccggcag agatagatat tgacatggaa aagatgaaga aactgttcac ccgccggcgg
     5821 tacagggatg actacaagat actttcagcc gaagtaagga aactggggga aaccattccc
     5881 ccgctcataa atgcctacat gaacctgtct cccacaatgc ggacattcgg cacattcatc
     5941 aaccattcct tcggggaagt tgaggaaaca ggcataatga taaccctcag ggatatttac
     6001 gttgagaaaa taaacagaca cctggcttcc tataagaagg actttatgat tacatggttt
     6061 tcgcctgact cccagtagtt ttctgctgtt ttcgtttcat gttaccctca ctgcctgctc
     6121 aggcccgggt aattgataac tgcc
//
LOCUS       DAOSPX010000189         6124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11792_length_6124_cov_4.768990, whole genome shotgun sequence.
ACCESSION   DAOSPX010000189 DAOSPX010000000
VERSION     DAOSPX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11792_length_6124_cov_4.768990"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="PLZ75_09435"
     CDS             <1..1502
                     /locus_tag="PLZ75_09435"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72654.1"
                     /translation="SQGDEIVADGYQVFRYPNGTISSEGYMKNGKPEGYWKSYYVTGV
                     KKSEGKRTSFLLDSIWIFYDQVGDTTEKISYLLGKKNGYYLRYKKDVTSGLYLYSREL
                     FAGDRKEGTAYIYYPDGRVQNTIQYSGGRKEGLSKEYDRNGEIITLLEYNNDFLISRE
                     RINRTDSKGLKQGPWKEFYPSGTIRTERNYKDDLVHGYYKEYDIRGRLTLTMLYDNGA
                     IVKSRVEDEPDIEIVNRYDSENKLIYSGPFRNNTPVGTHREYGKDGKVINAFIYNDNG
                     LLISEGIVDEAGNSSGKWKNFYPDGTVMAEGTYTDNRRSGAWKFYNSAGKTEQTGNYS
                     YGRPDGIWKWYYNDGSLLREEEYFQGEREGVFVEYAKDGEIIAEGQYADGEKNGHWKT
                     GTDFYREEGNYIIGLKDGQWKAFYSDGTLKFRGNYVQGNPEGQHFYYFDTGRLKEEQF
                     YRMGLRQRTWKKYDEVGNVILTITYKDDTEVSINGVRIDLPESDTKLIK"
     gene            1515..2540
                     /gene="ruvB"
                     /locus_tag="PLZ75_09440"
     CDS             1515..2540
                     /gene="ruvB"
                     /locus_tag="PLZ75_09440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOS72655.1"
                     /translation="MEEKFNIRKEGESVEELEFEKQLRPLFFYDFKGQRKIIDNLEVF
                     VKAAGQRNESLDHVLLHGPPGLGKTTLAAIIANELKVGFRVTSGPVIDKPGDLAGLLT
                     NLAEGDVLFIDEIHRLSPVVEEYLYSAMEDFKIDIVIDRGPGARSVQLSLNKFTLIGA
                     TTRSGLLTSPLRARFGIKFHLEYYDHEILSGIVKRSAGILKIEIDDAAAGEIAKRSRG
                     TPRVANALLRRIRDFAQVKGTGKIDLEITQYGLNALNIDIYGLDEMDNKILSAVVSKF
                     GGGPVGLNTIATAVGEESGTIEEVYEPFLIKEGFLKRTPRGREATELAYRHLGVIYRK
                     DLNGRLF"
     gene            complement(2557..3264)
                     /locus_tag="PLZ75_09445"
     CDS             complement(2557..3264)
                     /locus_tag="PLZ75_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOS72656.1"
                     /translation="MKEEDSFPLCDKCAPEAGSIFRHLLPAELEQLNYRKEFRKFRRS
                     DVLYREGGRISGFYCVHSGIIKVFKTGSDGKEQIIRFAKTGDIIAYRSVLSNEAACTT
                     AKVIADCEVCFIPADILISFVKSNPVFAHELLKLACHELGEANTFITDIAQKTVRERL
                     AEILLYLVRDFGLDENNYLRISLTREEIANIIGTATESVIRLLSEFKNDGFIELQGRR
                     IRILNSRALENIHNHLA"
     gene            complement(3319..4359)
                     /gene="pheS"
                     /locus_tag="PLZ75_09450"
     CDS             complement(3319..4359)
                     /gene="pheS"
                     /locus_tag="PLZ75_09450"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOS72657.1"
                     /translation="MLEKIDRLLSLISELSVKDIQELETFRIRYMSKKGIISELFEEF
                     RQMPASEKKEIGRKLNILKQSVIEKYDSYKDSLSLLPSSPRLNDPSRPSFPCSLGTRH
                     PISIVRGEIISIFERIGFSVSEGPEIESDDNNFTRLNFAPEHPARDMQDTFYISRISA
                     GDSNPGDILLRTHTSSVQVRVMQKQKPPIRTISPGRVFRNEAISARAHCIFHQVEGLY
                     IDENVSFADLKQTLLFFAREMYGEKTEIRFRPSYFPFTEPSGEMDVSCTICGGKGCNV
                     CKYTGWLEVLGCGMVHPAVLEASNIDSTKYTGFAFGMGIERPAMLKYQVNDLRMYYEN
                     DLRFLEQFRSAF"
     gene            complement(4384..5199)
                     /locus_tag="PLZ75_09455"
     CDS             complement(4384..5199)
                     /locus_tag="PLZ75_09455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS72658.1"
                     /translation="MYKASLPALFLFLLNGILCCKSPNSSENTHILMQTTHGDIKLRL
                     YDDTPLHRDNFIKLTQSGIYEGISFHRVINSFMIQAGDPLTRQAGLPSGSDTLLTYTV
                     PAEFNSLHFHKKGALAAARQGNEVNPEMRSSGTQFYIVQGTVQTDAGLDLEEQRINNG
                     IRQAMFVRFIRQVADSAVKAGLSPDAGEIHEKASSLMFEYLTTNGNYVISDEQRAVYK
                     TAGGVPRLDGTYTVFGEVTEGLDVVDRIAAVKTDTHDRPLTDVRILKMKIISK"
     gene            5358..>6124
                     /locus_tag="PLZ75_09460"
     CDS             5358..>6124
                     /locus_tag="PLZ75_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOS72659.1"
                     /translation="MNEVRTLARQTFIYGFGTIVPRFLNYGILTFLYTRLFDKGEYGV
                     VTELYGWMVFLLIILTYGMETGFFRYAQKEEDYERVYSTAMISLLVSSSLFLLLVNIF
                     ISPVSVALRYSDNQGYIRLFAAIVATDAFTAIPFARLRKENKPVLFSTIKILNVVITI
                     GAVLLLLVIAPRIYERGGKALAGLYNPDYGVGYVFVANLIGSLSTLIILLPYTFRIKV
                     LFDRSLWFRMVSYSFPLMIAGLGGAVNETLDKVIMRRV"
BASE COUNT         1808 a         1184 c         1489 g         1643 t
ORIGIN      
        1 tttcccaggg cgatgagatc gttgcagacg gatatcaggt attccgctat cccaacggga
       61 caatctcaag cgaggggtac atgaaaaacg ggaaacccga aggttactgg aaaagctatt
      121 atgttaccgg agtgaagaaa tctgagggaa agcgtaccag ttttctgctt gacagtatat
      181 ggatattcta tgaccaggta ggtgatacca ctgaaaagat aagttacctg ctggggaaga
      241 aaaacggata ttatctgaga tataaaaagg atgtgacttc aggactgtat ctctattcaa
      301 gggaactgtt tgcaggagac aggaaagaag ggactgccta tatatactat cctgacggca
      361 gggttcagaa tacaatccag tacagcgggg gaagaaaaga gggtttgtcg aaggaatatg
      421 acagaaacgg ggaaatcatt acactgctgg aatataacaa cgattttctg atcagcaggg
      481 aaaggattaa cagaactgat tccaaaggac ttaaacaggg cccgtggaag gaattttacc
      541 cgtccggaac tatcaggacc gaaaggaatt acaaggatga tctcgttcat ggctattaca
      601 aggaatatga catcagggga aggctgacgc ttacaatgct gtatgacaac ggggcaattg
      661 taaaatcgag ggtggaagac gaacccgata ttgaaatcgt taaccgttac gacagtgaaa
      721 acaagctaat atacagtgga ccgttcagga ataacactcc cgtgggaact cacagggaat
      781 acggaaagga cggaaaagta atcaatgcct ttatctataa tgacaatgga ttactgattt
      841 ctgagggaat tgtggatgag gccggaaatt ccagcggaaa atggaaaaac ttttaccccg
      901 acggaacggt gatggctgag ggaacatata cggataacag gcgcagcggt gcatggaagt
      961 tctataacag tgccggaaaa actgaacaga caggaaacta tagttacggg aggccggacg
     1021 gaatatggaa atggtattac aatgacgggt ctttgctcag ggaagaggaa tatttccagg
     1081 gagaaaggga aggtgttttt gtggaatatg cgaaagacgg ggaaataatt gcagaaggac
     1141 agtatgctga cggtgaaaag aatggtcact ggaaaaccgg gacggatttt tacagagagg
     1201 aaggaaatta tatcatcggc cttaaggacg gtcagtggaa agcattttac agtgacggaa
     1261 cccttaagtt caggggaaat tatgtgcagg gaaatcctga aggacagcat ttttactatt
     1321 ttgataccgg caggctgaag gaagaacagt tttaccgtat gggattaaga cagaggacct
     1381 ggaaaaagta tgatgaggta ggaaatgtta tactgaccat aacatacaag gatgacacgg
     1441 aagtcagtat aaacggggta cgaattgatc tgccggaaag cgataccaag ctgattaaat
     1501 agtaaatcct ttacatggaa gagaaattta atataagaaa agagggagag tcggtagaag
     1561 aacttgaatt tgagaaacag ctgagacctc ttttctttta tgatttcaag gggcagagaa
     1621 aaataataga taatctggaa gtctttgtca aggctgccgg acaaaggaat gaatcacttg
     1681 atcatgttct gcttcacgga ccacccggac ttggtaaaac aaccctggca gctattattg
     1741 caaatgaact gaaagtaggg ttcagggtta catccggacc ggttattgat aagccggggg
     1801 accttgccgg actacttaca aatctcgcgg aaggagatgt gcttttcata gatgaaatcc
     1861 acaggctgtc tcctgttgtt gaggagtatc tgtattcggc aatggaagat ttcaagattg
     1921 atatagttat agacaggggg cctggagcaa gatcggttca gctgagcctg aataaattta
     1981 ccctgatagg ggccacaacg aggtcaggat tgctgacaag cccgctcagg gcgcgttttg
     2041 gaatcaagtt ccacctggaa tattatgatc atgaaattct ttcaggcatc gttaaaagat
     2101 cagccggaat actgaaaatt gaaatagatg atgctgccgc aggggagatt gccaaaagga
     2161 gcagggggac accaagagtg gcaaacgctc ttctgagacg aataagggat tttgcccagg
     2221 tgaagggaac ggggaaaatt gacctggaaa ttactcagta tggccttaat gcactgaata
     2281 ttgacatata cggactggat gagatggata ataagatact ttcagctgtt gtcagtaaat
     2341 tcggtggcgg gccggtggga ctaaatacga ttgctacagc tgtaggtgag gaaagcggga
     2401 ctatagaaga ggtttatgaa ccatttctca ttaaggaagg attcctgaaa aggactccgc
     2461 gtggcaggga agcaacggaa cttgcctaca ggcatcttgg tgtgatttac aggaaggacc
     2521 tgaacggcag gcttttctga taaaatacag gaaaaatcag gcaagatggt tgtgtatgtt
     2581 ttcaagtgcc ctggagttca gaatacggat cctccttccc tggagttcta taaagccatc
     2641 gtttttaaat tcagaaagta aacgtataac cgattctgtt gcggtaccga taatattcgc
     2701 aatctcttcg cgggtgaggg aaatccggag ataattgttt tcatcaagac caaagtccct
     2761 gacgaggtaa agcagtattt cggcaagtct ttcccgaacg gttttctggg ctatatcggt
     2821 aataaaagta ttcgcttcac cgagttcatg gcaggcaagc ttcagaagct catgagcgaa
     2881 aaccggattt gattttacaa atgaaatgag gatatcggcc gggatgaaac aaacttcaca
     2941 gtctgcgatt actttagctg tggtacaggc agcttcattg ctcaaaacgc tcctgtaggc
     3001 tattatatca ccggttttgg caaagcggat tatctgttcc ttgccgtcag aacctgtttt
     3061 gaaaactttg ataataccgc tgtgaacaca gtaaaatccg ctgatgcgcc cgccctcacg
     3121 gtacaggaca tcacttcgcc tgaatttcct gaattccttc ctgtaattga gctgttccag
     3181 ttctgcagga agcagatgcc ggaaaattga accggcttca ggtgcacatt tatcacaaag
     3241 aggaaaggaa tcttcttcct tcatttcatg aaaagacttt tctgtttaac acttatggta
     3301 aaaacaaagt tcaaaatatc aaaaggcaga tctaaactgt tcgagaaatc tgaggtcgtt
     3361 ttcataatac atacgcagat cattgacctg atatttgagc attgccggtc tttctatgcc
     3421 catcccgaaa gcaaaaccgg tatatttcgt gctgtcgata ttgctggctt ccagtacggc
     3481 gggatggacc ataccgcatc ccagaacttc aagccagccg gtatatttac atacattgca
     3541 acccttacca ccgcatattg tgcaacttac atccatctca cccgaaggct cggtaaacgg
     3601 gaaataggat ggcctgaacc ggatttcagt cttttctccg tacatctccc tggcaaagaa
     3661 aaggagggtt tgcttgagat cggcaaacga aacattttca tcgatatata atccttccac
     3721 ctggtgaaag atacagtggg cccttgctga aattgcctca ttcctgaaaa ccctgccggg
     3781 ggagatagtc cttatcggag gcttctgttt ctgcataacc cttacctgta ctgaggaagt
     3841 atgagtccgg agaagaatat cacccggatt tgaatccccg gcagaaatgc gggaaatata
     3901 aaatgtatcc tgcatatccc ttgcaggatg ttcgggtgcg aaattaagac gggtaaaatt
     3961 gttatcatca ctttctattt caggaccttc cgatacgctg aaacctattc tttcaaaaat
     4021 acttatgatt tcacccctca ctatggatat aggatgccgg gttccgagac tgcagggaaa
     4081 agacggcctg gaaggatcat tcaggcgggg ggaagaaggt agaagcgaca gggaatcctt
     4141 gtatgaatcg tatttttcaa ttacagactg cttaagtata ttgagtttcc tgcctatttc
     4201 ctttttctcg gatgcgggca tctgcctgaa ctcctcaaaa agttcggaaa taattccctt
     4261 tttgctcata tacctgatac ggaatgtttc aagttcctgg atatctttaa ctgaaagttc
     4321 cgatatgagc gatagtaacc tgtctatttt ctcaagcatt gacaatcccc tgttttagat
     4381 gaattatttt gatatgattt tcatttttaa tattctcacg tctgtaagcg ggcggtcatg
     4441 agtatctgtt ttgactgctg caatcctgtc aacaacatcc agaccttcag ttacctcgcc
     4501 aaaaacggta taggtgccgt caagccgggg aacacccccc gcagtcttgt aaacagctcg
     4561 ctgttcatcg ctgatgacat agtttccgtt tgtggtcagg tattcaaaca tcagtgagga
     4621 tgctttttcg tgaatctcgc ctgcgtccgg actgagacct gcttttacgg cactgtccgc
     4681 aacctggcgg ataaaacgga caaacattgc ctgtcttatg ccgttattga tcctttgttc
     4741 ttcaagatcg aggccggcat ctgtctgtac cgtgccctgg acaatataaa actgggtacc
     4801 ggatgaccgc atttcggggt ttacttcatt tccctgacgt gcagctgcaa gtgcaccctt
     4861 cttgtggaaa tgaagggaat taaactcagc gggaacggta taggtcaaca aggtatctga
     4921 gccggatgga agacctgcct gccgggtcag cggatcaccg gcctgtatca taaaactgtt
     4981 tattacgcgg tgaaaggaaa taccctcata tataccggat tgggttagtt ttataaaatt
     5041 gtcacggtga agaggggtat catcatataa tctgagcttt atatccccat gggttgtttg
     5101 catcagaata tgtgtattct cgctggaatt aggagattta cagcataata tcccgttcag
     5161 aaggaacagg aaaagtgccg gaagggaagc tttatacatg tcagactcct ttgatatata
     5221 agtgaattaa ccggagggca aagatattca actttaaact attatggtaa gttttatgaa
     5281 aattgtgtgc ataaatgatt gcaggctgga tgaaaaaatc gaaatcttaa acctatattt
     5341 gtaatcattt taataaagtg aacgaagtca ggacattagc cagacaaaca tttatttacg
     5401 gattcggaac cattgttccc cggttcctta attacggaat actgaccttt ctatatacaa
     5461 ggctttttga caagggagag tatggtgtag taacagagtt atacggatgg atggtctttc
     5521 ttcttatcat tctgacctac ggaatggaaa cgggtttttt ccggtatgct cagaaggaag
     5581 aagattatga aagggtttat tccacggcca tgatcagcct tcttgtttcc tcctccctct
     5641 tccttctgct tgtaaatatt ttcattagtc ctgtttctgt tgcgctcaga tatagtgata
     5701 atcagggata catccgtctt tttgctgcaa ttgttgctac tgatgccttt acagcaatac
     5761 cttttgcaag gttaagaaag gaaaacaagc ccgtgctttt cagtacaatt aaaatactga
     5821 acgttgtaat tactatcggg gctgttttat tactgcttgt catagcaccg cgtatatatg
     5881 aaaggggagg gaaggccctt gcaggcctat ataatcctga ttacggtgtg ggctatgtgt
     5941 ttgttgccaa tctgatagga agcctttcaa cgctgataat acttcttccc tatacattca
     6001 ggattaaagt tttattcgac cggagtttgt ggttcagaat ggtctcctat tcctttccgc
     6061 tgatgattgc aggacttggc ggtgcagtaa atgagactct tgacaaggtt ataatgcgca
     6121 gggt
//
LOCUS       DAOSPX010000190         6117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11812_length_6117_cov_4.353184, whole genome shotgun sequence.
ACCESSION   DAOSPX010000190 DAOSPX010000000
VERSION     DAOSPX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11812_length_6117_cov_4.353184"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1913
                     /locus_tag="PLZ75_09465"
     CDS             <1..1913
                     /locus_tag="PLZ75_09465"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOS72660.1"
                     /translation="CIALWFAGLLLYTLEKDRITPLSPDEAIFAGTLEEIPEEKENTW
                     KMLIRLKGFISGDSLRPAGGSLLIYHRKEGRISSFLPGDQLLIRCRPEKIRNMGNPSE
                     FDYSFYMERKGIRYLSFTDSTCIIARKIPERRKMKHSALIIRERILEIYRERGVSEKR
                     LGLVAAMTLGQKNLLDPEQKLYFIRAGVMHIMAVSGLHTVILSLFVFKMLFFLKGRMN
                     ILRIIITLTILWVYAFVTGLTPSVLRATLMYSFLQAGKAMRRDVNSVNSVLASAFVLI
                     LIRPSVIFDAGFLLSYSAVIFIICFYRDLYLKLNFRHRIPRMIWESAAVTITAQTGTL
                     PLTVMMFNRFPVWFILSNIIIVPLASLVIVSGALTIFFYPLPFLSRITGGFLEFITGL
                     TETLTETAASLPLSTIENIGIGTAQCILLSISLFLLLKYLLDRKSVRAAFPLSFLLLF
                     ITAGTIKDISTRRSGEIIVYNTAGHTTIGIRAGKHLNSYTATLITLKEVERHKAMAGL
                     KETKNLIKDNTLLKTGYRNVKNNTADMHILITERLTDSLLEAYAPDIIVLRGRNPLIE
                     DKINLNTVSDISFESSGNYTPANASTYISVNKSIRALVISPELQRRSGIKIPNADTVH
                     YVRTSGAFRLSL"
     gene            1997..3340
                     /gene="trkA"
                     /locus_tag="PLZ75_09470"
     CDS             1997..3340
                     /gene="trkA"
                     /locus_tag="PLZ75_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOS72661.1"
                     /translation="MRIVIAGAGEVGSHLARMLLNENHEIILIDPEDSSLKPIESTLD
                     VLTFHGSSTSVKILQEILHKKTDLFIAVTTHEDTNIISSILAKRFGALKTIARVDNLD
                     YLESSNIDFFKTLGVDSLIYPELIAAREVLGLLQEAGKTDYMEFSGGKLSVFIQRLDE
                     NAPIVNKSLREISLQFKTDQYRAVAVKRKDKTIIPRGSEQFLAGDLIYVISTSEGINE
                     MMKTSGKENHEVNSIMILGGSKIGKHVALYMQKNCEVKLIDSDPARCEELAEILDNTL
                     IINGDCRDAELLEQEGVSGMDAFIAVTDSSEINILSCLLAKKLGVRKTIAEVENMEYI
                     NLAENSGIDTIINKKLSAAGRIFRHTTTSLNVTQIRYMAGTDAEILEFNVPPGARITR
                     GTLRSIDFPEDSIVGGGTRDGVPFIATGDTIIKANDKVVVFTLPSAYDRLIKFFT"
     gene            3347..4795
                     /locus_tag="PLZ75_09475"
     CDS             3347..4795
                     /locus_tag="PLZ75_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOS72662.1"
                     /translation="MVNFRVITRMIGIVLIAEGFFMLLSAALSFILKESAATPLLFSS
                     IITLVSGILVFSPLRYEEKLSAYKEGYIITVGIWLSLSLFGTLPWLLSGSIKSFTDAF
                     FESMSGFTTTGATVLTDPGALPKGLVLWRSLSQWIGGIGFILISLSVLPVVKAVNIQL
                     TINDFTAQASGRIHPRTGETFKMLISVYVILTLAETVMLIAGGMNILDAFCISLSTLS
                     TGGFSPLRDSLAGIGSPYILIVITIFMFLAGTNLSLVYFGYRRNFGKIRSSNEFRFYG
                     ILCLVFILAMFLALWAKNIYPAGRSLIQGAFQAVSTITTTGFYNSDYNLWGGFLTLVI
                     FILMFTGGTSGSASGSLKIIRILLAGKNTRHEMKRLIHPDAVIPVRFDEKVVSRDLIY
                     NLLVFIILYLVVICISAFVVSFMGYDLITAFSTSAAMLGNIGPAPGAFGPGSTYSALP
                     AAGKWFFSFIMLLGRLEILSVLVLFTGNFYRR"
     gene            complement(4801..5415)
                     /locus_tag="PLZ75_09480"
     CDS             complement(4801..5415)
                     /locus_tag="PLZ75_09480"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOS72663.1"
                     /translation="MLLRIYPENPNVRHIRKVADVLEAGGIVIYPTDTVYAMGCDIRA
                     HKAIERIARLKGLNPDNPDLSIIFRDMSQLSEYTVIRDDNIFRLLKRNLPGPFTFIVK
                     ANNQIPRLFKNKKKTVGIRIPESKILHEIIKELGRPIITTSVHDPDEIIEYTTEPELI
                     WEKYRDFADIVINGGYGNNEASTIVDCTGDEIVIVRQGLGELEW"
     gene            complement(5440..5539)
                     /gene="ffs"
                     /locus_tag="PLZ75_09485"
     ncRNA           complement(5440..5539)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLZ75_09485"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5679..>6117
                     /locus_tag="PLZ75_09490"
     CDS             5679..>6117
                     /locus_tag="PLZ75_09490"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOS72664.1"
                     /translation="MDQNVNPWKANLTNGVIMALIGIAYSLVIYFLDLTLNKAQGWVF
                     MLIQIVILYFLLKSYRDNYRYGTISFSQGLGAGMIIFVYYAIIIAVFSYILYAWIDPD
                     LIDKQLAFAEEQMLEKGIPAEAVEAGMKVQAKLLKPPIMAVLSI"
BASE COUNT         1663 a         1443 c         1322 g         1689 t
ORIGIN      
        1 tttgtattgc cctgtggttc gcgggtcttt tactttacac cctggagaag gaccggatca
       61 cccccctttc tccggatgag gcaatatttg ccggaacgct tgaagaaata cctgaagaaa
      121 aggagaatac ctggaaaatg ctcatcaggc tgaagggctt cataagtggc gacagtctac
      181 ggcctgcagg agggtctttg cttatctacc acaggaaaga aggcagaatt tcctcattcc
      241 tgccgggcga tcagcttttg ataagatgcc ggcccgaaaa aatcaggaac atgggtaatc
      301 cctcggagtt cgactacagt ttctatatgg agagaaaagg aatcaggtat ctttcattta
      361 ccgacagcac ctgtattatt gcccgcaaaa ttcctgaacg caggaaaatg aaacattccg
      421 cactgattat ccgggagagg atacttgaaa tttaccgtga acgcggggtg agtgaaaagc
      481 gtctcggact ggttgcagcc atgacactgg ggcagaaaaa ccttctcgac ccggaacaga
      541 aactctattt catcagggcc ggggtaatgc atatcatggc agtatcaggc cttcacacgg
      601 ttatcctgag ccttttcgtc tttaaaatgc ttttcttcct gaaaggcaga atgaatatcc
      661 tcaggattat catcacttta accatcctgt gggtttatgc ctttgttacc ggactcacgc
      721 cctccgttct gagggccact ctgatgtatt cctttcttca ggcaggaaag gcgatgagaa
      781 gagatgtcaa ttccgtgaat tccgtactgg cttcagcctt tgtcctgatc cttatcagac
      841 cctctgtcat tttcgacgcc ggattccttc tctcctactc agcagtgata tttataatct
      901 gcttctaccg tgatctgtac cttaaattaa atttcaggca tcgtattccc cgtatgatct
      961 gggagtcagc tgccgttacc ataaccgccc agacaggcac ccttcccctt acggtaatga
     1021 tgttcaacag atttcctgta tggttcatat tgtcaaatat tataatagtg cccctggcat
     1081 cgctggttat tgtcagcggg gccctgacaa tattttttta tccgcttccg ttcctttcgc
     1141 ggattacagg aggttttctc gaattcatta ccggactgac tgaaactctt acagaaacag
     1201 ctgcctccct tccgctttca acaatagaaa atatcggaat aggcactgct caatgcattc
     1261 tgctttcaat aagcctcttt ttgctgctga aatatcttct tgacaggaaa tcagtcaggg
     1321 cagcctttcc tctgagcttc ctcctgcttt tcattacggc aggcaccata aaggatataa
     1381 gtaccaggag aagtggcgaa atcattgtat acaacactgc aggccatacc accatcggga
     1441 tcagggcggg caaacatctg aacagctata ccgctaccct tataacgctg aaggaagtgg
     1501 aaaggcacaa ggccatggca ggcctgaagg aaacgaaaaa tctgataaag gataataccc
     1561 ttttaaaaac cggatacagg aacgtaaaga acaatacggc ggatatgcat atcctgatca
     1621 ccgaaaggct cacagattca ttacttgagg catacgctcc cgatataata gtcctcaggg
     1681 gcaggaatcc actgattgag gataagatca atctgaatac agtcagtgac attagttttg
     1741 aaagttccgg caattacact cctgccaatg ccagcactta tatttctgtc aacaaaagta
     1801 tcagggccct ggtaattagt cctgagttac agcgccgttc aggaataaaa atccctaatg
     1861 ctgataccgt gcattatgta aggacgtccg gtgctttccg cctcagcttg tgaggcacag
     1921 ggaaataaat attccggaaa acttgagtat tatctcattt atgccttatt ttgtgcagtt
     1981 ttttcattca tttatcatga gaatagtaat tgccggcgca ggtgaagttg gctctcatct
     2041 tgcaaggatg cttttgaatg aaaatcatga aatcattctg atcgacccgg aagactcaag
     2101 tctcaaacca atagaatcaa cactcgatgt actgaccttt cacggatctt caacatccgt
     2161 caagatcctt caggagatcc tgcataaaaa gactgatctg ttcatagccg tgacaaccca
     2221 cgaggatacg aatattattt cctccatcct ggcaaagcgt tttggtgcac tgaagacaat
     2281 tgcccgtgtt gacaatctcg attacctgga atcttcaaat attgattttt tcaagactct
     2341 gggtgtcgat tcgctgatct atcccgaact catagccgcc agggaggtac tcggacttct
     2401 tcaggaagca ggaaaaactg actacatgga gttctcagga gggaaacttt cagtcttcat
     2461 acaacgtctc gacgagaatg cccccattgt caataaaagt ctcagggaaa tatccttaca
     2521 attcaaaacc gaccagtacc gtgccgttgc cgtaaaacga aaagacaaga ccatcattcc
     2581 cagggggagc gaacaattcc tggcaggaga tctcatctat gtcatttcca caagtgaagg
     2641 gatcaatgag atgatgaaaa cttccggaaa ggagaatcac gaagtgaaca gcatcatgat
     2701 tctgggtggc agcaaaatag gcaagcatgt ggctctttac atgcagaaaa actgcgaagt
     2761 gaagcttatc gactcagatc cggcacgatg tgaagaactt gcggaaatac ttgacaacac
     2821 actgataata aatggcgact gcagggatgc cgaacttctt gaacaggaag gcgtttcagg
     2881 catggatgcg tttattgccg tcactgacag ttcagaaatc aatattctgt catgtcttct
     2941 ggccaagaag cttggtgtga gaaaaaccat tgcggaagtt gaaaacatgg aatacatcaa
     3001 tcttgcagaa aattcgggga ttgacaccat cataaacaag aagctttcgg ccgcgggcag
     3061 gatcttcaga catacaacca cttccctcaa tgttacacag ataaggtaca tggcaggaac
     3121 cgatgctgaa attcttgagt ttaacgttcc gcccggagcc aggataaccc gcggtactct
     3181 ccgcagcatt gattttcctg aagattccat tgtcgggggc ggaacccgcg acggagtacc
     3241 ttttattgcc acgggtgaca ccattattaa ggccaatgac aaagtggtgg tgttcacact
     3301 gccttcagca tacgaccgtc tgataaaatt ctttacctga tacagtatgg tcaatttcag
     3361 ggtcattaca agaatgatcg gcatcgtact gattgcggaa ggatttttca tgcttctttc
     3421 cgccgctctt tcctttatcc tgaaagaatc tgccgcaacc cctctgttgt tttcctccat
     3481 cataaccctg gtttcaggca ttctggtctt ttcacccctc agatatgaag aaaagcttag
     3541 cgcctacaag gagggatata tcatcaccgt tggtatctgg ctttctttaa gcctgttcgg
     3601 aacactgccc tggctgctca gcggttccat aaaaagcttc accgatgcct tttttgaatc
     3661 aatgtcaggt ttcacaacca ccggtgccac agtcctgaca gatcccggag ccctgccaaa
     3721 gggtcttgtt ctatggagaa gcctcagcca gtggatagga ggaattggat tcattctgat
     3781 ttcattgtcg gttcttcctg ttgtgaaggc agtaaacata cagcttacaa taaacgattt
     3841 cactgcccag gcatccggca ggatccaccc ccgcacaggg gagactttta aaatgctcat
     3901 ctctgtttat gttatcctga ctcttgctga aactgttatg ctgattgccg gaggaatgaa
     3961 tatcctggat gcattctgca tttccctgtc aactttatca acaggcggct tctcgcccct
     4021 cagggacagt cttgccggca tcggatcacc ctatatcctt atcgtaataa caatattcat
     4081 gttcctggcc ggaacaaatc tttcacttgt atatttcgga tacaggagaa atttcgggaa
     4141 aataagatcc agcaatgaat tcaggttcta tggaatcctt tgccttgtgt tcattcttgc
     4201 aatgttcctt gctctatggg caaaaaacat ttaccctgcg ggcaggtcgc tgattcaggg
     4261 ggctttccag gctgtatcaa caataacaac aacaggattc tacaattctg actataacct
     4321 ctggggcggc ttcctcacac tggtaatctt cattctgatg ttcacaggcg gaacttccgg
     4381 ttcggccagc ggtagtctta aaataataag gatactgctt gcagggaaaa atacacgtca
     4441 cgaaatgaag cggcttatcc accctgatgc cgttattccc gtgaggtttg acgaaaaagt
     4501 ggtatcccgt gatcttatct acaatctgct tgtattcatt atactatacc ttgtggtaat
     4561 ctgcatttca gcctttgtcg tttctttcat gggttatgac ctgataaccg cattcagcac
     4621 ttctgccgca atgctgggga atattggtcc ggctccgggt gcatttggtc cgggatcaac
     4681 atactctgcc ctgcctgcag ccggcaaatg gttcttctca tttattatgc tgctgggccg
     4741 tctggaaatc ctttccgtac tggtactctt taccggaaat ttctaccgga gatgacggtt
     4801 ctaccattca agctcaccaa gaccctgtct gactataaca atttcatccc cggtacagtc
     4861 aacaatcgtt gaggcctcat tgttgccata cccgccattg atcacgatat cggcgaaatc
     4921 acggtatttc tcccagatca gctcaggttc ggtggtatat tcaattatct cgtcagggtc
     4981 atgcactgaa gttgtaataa taggacgccc cagttccttg ataatctcat gaaggatttt
     5041 gctttccggg atacggatac ccacagtttt cttcttattc ttgaacagcc tgggtatctg
     5101 attgtttgcc ttaacaatga aggtaaaggg tcccggaagg tttcgcttca gaagcctgaa
     5161 aatattgtcg tcacgtatta ccgtgtactc ggacagctgg ctcatatcgc ggaatatgat
     5221 ggaaagatcc ggattgtcgg gattcagtcc tttcagtctt gcaatccttt caattgcctt
     5281 gtgagccctg atatcgcaac ccatggcata aaccgtgtcg gtcggataaa taactatgcc
     5341 accggcttcc agtacatccg caaccttgcg tatatgccgc acgttgggat tctcaggata
     5401 aattcgcaat aacatgttaa ttaaattcag gcatttaaaa aagggtaagc tacttatatc
     5461 gcatcgctac aaccgcctgc ccttgctacc ttccgatcct gggggagttc agcaggagct
     5521 ggtcgtataa gacttacccg acgcaaaaat acaataatta agattacctt caaaatattt
     5581 atactgttgc accaggcagg gcattctcaa ttttacatta tatttgttta atatgaaagg
     5641 aagaaaaagc cctaataatt cataataaag cacaaacaat ggatcaaaat gtaaacccct
     5701 ggaaagcaaa ccttacaaac ggagtgatca tggctcttat cggaatagcc tatagcctgg
     5761 ttatctattt tctcgatctc accttgaata aagcacaagg ctgggtattc atgctgatac
     5821 agatagtaat cctttatttc cttctgaaat cctatcgcga taactacaga tacggaacta
     5881 tcagcttctc ccagggcctg ggtgcaggaa tgataatttt tgtctattac gcaataatta
     5941 ttgccgtgtt ttcatacatc ctctatgcct ggatagatcc cgatcttatt gacaagcaac
     6001 tggcatttgc tgaagaacaa atgcttgaga aagggatacc tgcggaggcg gttgaagccg
     6061 gaatgaaagt tcaggcgaaa ctgctgaagc cgccgataat ggctgtttta agcattt
//
LOCUS       DAOSPX010000191         6116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11815_length_6116_cov_4.283617, whole genome shotgun sequence.
ACCESSION   DAOSPX010000191 DAOSPX010000000
VERSION     DAOSPX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11815_length_6116_cov_4.283617"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3005)
                     /locus_tag="PLZ75_09495"
     CDS             complement(<1..3005)
                     /locus_tag="PLZ75_09495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOS72665.1"
                     /translation="MSNESVVFIDTEIGGHSGQILDYGAIRNDGAKCRTRSGAVFHGF
                     IKDAAFLCGHNIVHHDLEYIKQQVHGFTIAQNSIIDTLFWSPLLFPRKPYHRLIKDDK
                     LIPEDINNPVSDAIKSRDLLYDEIHAFNDLPEELRNIFYSLLGGFSGFAGFFRFISFS
                     PPLSLPAAKLIRSYFSGSICSNCNLDDLILFKPIELAYSLAIINANDRDSITPPWVLK
                     TFPETVEVIQVLRDTPCIQDCTYCSSVMNPVNGLKKYFGFSEFRKFGGEPLQEQAVKA
                     AVDNKSILAVFPTGGGKSLAFQLPALISGETVKGLTVVISPLQSLMKDQVDNLENKKI
                     TEAVAVSGLLDPIERQDALNRVVNGSASILYIAPESLRTASIRHRLIGRNIVRFVIDE
                     AHCFSAWGHDFRVDYMYIGRFIKELQEDKGLSRPVPVSCFTATAKRKVIEDIRAYFKA
                     ILDLDLELFTTDETRQNLYYKVIEVKNDRDKFFRLREILERYDCPSIVYVARTKKAEE
                     LAYQLGENGINALPFHGRMDRDVKTRNQEAFMAGDVNTMVATSAFGMGVDKDNIGLII
                     HYDISDSLENYIQEAGRAGRDFSITADCYILFNDDDLAKHFHNHNRQKVHIKEIQQVW
                     RAIRTISRMREKISYSALELAREAGWEDDTGAELETRVLTVLSALEMARFIKRDKNNP
                     RIYANSIQVRSADEAINIISGSERFSDKEREYATRIIKALISSRSRKRSSDELAESRI
                     DYLSDNLGIRKEEVVRTVMLLREEKILADSKDMRAFIFAKDSENGSLSILEIFAGIEK
                     FLFTKFRDDVIAYSVKDLNEEASVNGCTGINPRRIKTLTNFWTLRNWVEYYGNRSGDH
                     MLRIRKRMPDELTSDKIQRRHETASFIIRYLFRKPDSGATGQGYRIVEFSILELKNML
                     KTEFALFKDSVSVVEIEDTLLYLSRIGAIKIDGAFFVIYNRLTIERLQLNNRIQYKSD
                     DYRQLEEHYANKVQQIHIVGEYAKKMLHD"
     assembly_gap    3010..3019
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3381..4697)
                     /locus_tag="PLZ75_09500"
     CDS             complement(3381..4697)
                     /locus_tag="PLZ75_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72666.1"
                     /translation="MKTKKLNHGIYTGIIMLILFMIPVSVSDACFTAKVKDTNVNVQL
                     PIAELQGLIQQLQDAGSQLVGEAGVQIRQSIAELSAEMEQRIDQIQNAGSELIAEASA
                     EITSIINNLIGKAKELLSEVNRMIQQNIQCIDYVLAQRIQQITDAAYGIMDKIDSTLK
                     NAIDRIYIRASMLVDTSTHRVALVVNNTLKIVVKIIIFIFCFLLLFWLIKMLWKSTFP
                     KSKVLAIGIPVLIVLLVGAGTFLMLSKTALARMIGEEIPIPKWEESCDRGSSFYTSFI
                     SMKNEGKSIEELKPIGNTALEELNWCLYASVSPEATIDTYDKISTIKAILYPPVSAPP
                     APGTVITGSPCGSQGSIDPGWFSNRNFTKVEMWKKLSDNNIIRFSSVINPSDYKARVN
                     YIDPVIILKPEINPIIDYKPPTDFNRSKINDKIIKQQGTRVRQKDF"
     gene            4936..5163
                     /locus_tag="PLZ75_09505"
     CDS             4936..5163
                     /locus_tag="PLZ75_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72667.1"
                     /translation="MIKVPLKLTFIYSLTEELTALFRSLSDTDADPALPESLISTLIS
                     LGVLLQPSQDWNKKAIKKNNDPIPIIAKVDF"
     gene            complement(5219..6094)
                     /gene="lnt"
                     /locus_tag="PLZ75_09510"
     CDS             complement(5219..6094)
                     /gene="lnt"
                     /locus_tag="PLZ75_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOS72668.1"
                     /translation="MPDKYNMDNEKLLKHYLSVSSIYLTPNTDYLIWPETAFPDMVFI
                     DSLETDKIVDSIITLYRNTDTKIITGFVLGEMGKPESGETEFTEYNAAVQIIPSQKRI
                     DIKTKDKCVPFTERIPYPKILSFLSNYIEGLAGYRFSKSGNYGKVNFISDNIRVVPSI
                     CYESAFCDINLPLRFNENTFIAVILNEGWYNNLRVAGQFMHLAVLRAIEYRKPVVRSS
                     NMGISCTVSSLGECSHILSRYGGKGFKAVIQANNYNSFFARHHRTCEQIIISFFFIFL
                     LFFFVPDFRYFVNLY"
BASE COUNT         1730 a         1281 c         1201 g         1894 t
ORIGIN      
        1 taatcatgaa gcattttttt ggcatactca cccactatat gtatttgctg taccttgttg
       61 gcataatgct cttcaagctg cctgtaatca tcacttttgt actggatcct gttatttaac
      121 tgaagacgtt caatagtcag acgattgtat attacaaaga aagctccgtc gattttaatt
      181 gcccctatcc ttgacagata aagcagtgta tcttctatct ccacaactga aaccgaatcc
      241 ttaaaaagtg cgaattcagt tttcagcata tttttcagtt ccaggatgga gaattcgact
      301 atcctgtatc cctggcccgt agccccagaa tcaggcttac ggaacaaata ccggataata
      361 aatgaagctg tttcatgccg cctctggatt ttatctgaag taagttcatc aggcatccgt
      421 tttctgatcc ggagcatatg gtccccgctt ctgtttccgt aatattctac ccagttccgc
      481 aacgtccaga aattggttaa tgtttttatt ctcctgggat tgatacctgt acaaccattt
      541 actgaagcct cttcattcag atcttttacg gaataagcaa tgacatcatc tctgaatttg
      601 gtgaacagaa atttttcaat cccggcaaag atttcaagga ttgaaaggga tccgttttca
      661 ctgtcttttg cgaaaataaa ggctcgcatg tctttgctgt cagccagtat tttttcttcc
      721 cttaaaagca tgacggtcct gacaacttct tcttttcgga ttcccagatt atcagacaaa
      781 tagtcgattc ttgattcagc aagttcatca ctggaccttt tccggctccg gcttgagatt
      841 aatgctttta ttattcttgt ggcgtattct ctttccttgt ctgagaagcg ttctgatccg
      901 ctgataatgt taatagcctc atctgcagat ctgacctgga tactgttagc atatatcctt
      961 ggattattct tgtctctttt gataaaacgg gccatttcca gggcagagag cactgtcaac
     1021 acacgtgtct ccagttcagc tcctgtatca tcttcccatc ctgcttctct tgctaattca
     1081 agagcggagt aggatatttt ctccctcatt ctggaaatgg ttcttatggc acgccatacc
     1141 tgctgaatct ccttgatgtg aaccttctgc cggttatgat tatgaaaatg cttagccaga
     1201 tcatcatcgt taaacagtat gtaacagtct gcggtaatcg aaaaatccct gcctgccctg
     1261 cccgcctcct gaatataatt ttccagtgaa tctgatatgt cataatggat tataagcccg
     1321 atgttatcct tgtcaactcc cattccgaaa gcagaggtgg ccaccatggt gttaacatct
     1381 cctgccataa aggcttcctg attgcgggtt ttgacatcac gatccatcct gccgtggaaa
     1441 ggcagggcat ttattccgtt ttcacccagt tgataagcaa gttcttctgc tttttttgtc
     1501 ctggctacat agactattga aggacagtca taccgttcaa ggatttcacg cagtctgaaa
     1561 aatttgtcgc ggtcgttctt gacttcgata accttgtagt acagattctg tctggtttca
     1621 tcggtggtga aaagctcaag atcaagatcc agtattgcct tgaaatatgc tcttatgtct
     1681 tcaataacct ttcttttggc agtagccgta aaacatgaaa caggaacagg ccgggaaaga
     1741 cccttgtctt cctgaagctc ttttatgaac cggccaatat acatgtagtc aacacggaaa
     1801 tcatgtcccc aggcagaaaa acaatgtgcc tcgtcaatga cgaatcttac tatgttcctg
     1861 ccgatgagcc tgtgtctgat ggatgctgta cgcaatgact caggagcaat atagagtatg
     1921 gatgccgatc cgttcaccac ccggttaagt gcatcctgcc gttcgatcgg atcaagcagt
     1981 ccggaaacag ccactgcttc agtaatcttc ttattttcaa gattatccac ctgatctttc
     2041 ataagtgact gaagcgggga aataaccacg gtaagtcctt ttacggtttc gccgcttata
     2101 agggccggaa gctgaaatgc cagagatttt ccacccccgg tcggaaatac agccagaatg
     2161 gatttgttgt caacagctgc ttttacagcc tgctcctgga gaggttctcc cccgaatttc
     2221 ctgaactctg aaaaaccgaa atacttttta agtccgttga caggattcat cactgagctg
     2281 caataagtgc aatcctgtat gcatggcgtg tctctcagaa cctgaatcac ctcaaccgtt
     2341 tcagggaaag tcttcagaac ccagggtggt gtgattgaat ctctgtcgtt ggcattaatg
     2401 attgccaggg aataggcaag ttcaattggc ttgaaaagta ttaaatcgtc aagattgcag
     2461 ttgctgcaga ttgatccact gaaataactt cttatcagtt tcgctgcagg caacgacagt
     2521 ggaggactga atgaaataaa gcggaagaat cctgcgaaac cggaaaaccc gcccagaaga
     2581 ctgtaaaaga tgtttctcag ctcttccgga agatcattaa aagcatgaat ctcatcatac
     2641 agcaggtcgc gcgacttgat agcgtcactg acagggttat taatatcttc ggggataagc
     2701 ttatcatctt ttatgagtct gtggtaaggc tttcggggaa aaagaagggg tgaccagaag
     2761 agtgtatcga tgatggagtt ttgcgcgatt gtgaaaccgt gaacctgctg ttttatatat
     2821 tcaaggtcat ggtggacaat gttatgaccg cataggaatg cagcatcttt aatgaatcca
     2881 tggaataccg cacctgatcg tgtccggcat ttggccccgt cattacgtat tgctccgtaa
     2941 tcgagaatct gaccgctatg gcccccgatt tcagtatcaa taaaaacaac agactcgttt
     3001 gacataatgn nnnnnnnnnt gaggaaaaag acttaaacct caacaaggag tacggataat
     3061 tatacggaat gttataccgc tttgatgtta tctatacaca tcttctgaaa atcaaagata
     3121 gccgtatttt ctgattcggt gacagtaaaa acaagtgact atcctttgtg ttctgaactt
     3181 ttaatatcct ggaggtttcc tttataaagt agatataacc ggattattcc ccggaagata
     3241 attattaaag aaaaattaca gagcactggg gaaatataaa ttcttcagag gaaaccaccc
     3301 tcatttttgc agtaaattgg gattcaaatg aaatattccc ctgcagatga agggattttt
     3361 taaaaggaaa tccggttatg ctaaaaatct ttttgtctta cccgggtacc ttgttgtttt
     3421 atgatcttgt catttatctt gcttctgtta aaatcagttg gcggtttata atcaataatc
     3481 ggattaattt ctggtttcaa tataattaca ggatcgatat aattgactct tgccttataa
     3541 tcggaaggat taattacact ggaaaatctg attatattat tatctgacag tttcttccac
     3601 atttccactt tggtgaaatt acgattgctg aaccatcccg gatctatgga accctgtgat
     3661 ccgcaaggac tgcctgtaat aactgtaccg ggagcaggag gagcagaaac gggaggatat
     3721 aagattgcct tgattgtact gatcttgtcg taggtatcaa ttgtagcttc agggctgaca
     3781 gatgcataca gacaccagtt taattcttcc agtgcagtgt ttcctattgg tttaagttct
     3841 tcaatactct ttccttcatt tttcatcgat ataaaactgg tatagaagct gcttcccctg
     3901 tcgcatgatt cttcccattt tggaattggt atttcctcac ctatcatgcg ggccagggct
     3961 gttttgctta acatcaggaa agtccccgca cccacaagca atactataag cacaggaata
     4021 ccgattgcca gaacttttga tttcgggaat gtacttttcc ataacatttt tatcagccag
     4081 aacaataaaa ggaagcaaaa gataaaaatg attatcttga caactatctt aagagtgttg
     4141 ttaacaacca gggctaccct gtgggtactg gtgtcaacca gcatggatgc cctgatatag
     4201 atacggtcaa tggcgttttt cagcgtggag tcaatcttat ccattatgcc ataggcagca
     4261 tctgttatct gctgtatcct ctgtgcaagc acataatcaa tacattggat attttgctga
     4321 atcattctgt taacttcaga taacagttcc ttcgctttac cgataaggtt atttatgatt
     4381 gaagtaatct ctgcagatgc ttctgcaatg agttcggatc ctgcattttg tatctgatcg
     4441 atccgctgct ccatttcggc actgagttca gctattgatt gtcttatctg aacccccgct
     4501 tcacctacca gctggctgcc tgcatcctga agttgctgta taagaccttg taattcagct
     4561 ataggcaact ggacattgac atttgtatcc tttaccttgg ctgtaaagca ggcatcactg
     4621 acagatacag gtatcataaa aagtataagc ataataatac ccgtataaat accgtggttg
     4681 agcttttttg ttttcatttg atttcgttgt tatttgcagt ttgactggcc ataggtaaaa
     4741 ttgaaggatg tggctgtttt ggggttgata accttggtgc atgacgcaga ccacgttttt
     4801 gcacttacag ttatttgcca gtttcccggt ttgaggttat aacgggtaat cgcaggaaca
     4861 ggtgacacat tgctactggt cacctcacag gttttttgcg tagtaaacga agtttgtccg
     4921 gttccttctc cggctgtgat taaagttcca ctgaaactta catttatata ctccttaacg
     4981 gaagaactca ccgctctgtt cagatcactc agcgatactg atgctgatcc ggccttgccc
     5041 gaatctttaa tatcaacgtt aatatcgctg ggtgttttac tgcagccttc acaggactgg
     5101 aataaaaaag caattaaaaa aaacaatgat ccaatcccga taatcgcaaa agtcgacttt
     5161 tgagaataac gtgccatttt gttcgggtat taagaaatgg aattaaaaaa aatgaatctc
     5221 aatataaatt tacgaaatac ctgaaatcag gaacaaaaaa gaaaagtaag aaaatgaaaa
     5281 aaaatgaaat gattatctgc tcgcaggtcc ggtgatgcct tgcaaaaaag ctattataat
     5341 tatttgcctg tataacagcc ttaaagcctt tcccgccata acgtgaaaga atatgactgc
     5401 attcaccaag ggaagagaca gtacatgaaa ttcccatgtt tgatgatctt actacaggtt
     5461 ttctatattc aatggccctt aaaacagcaa gatgcatgaa ttgaccggct acccgcagat
     5521 tattatacca tccttcatta agtattacag cgataaaagt attttcatta aatcgtaaag
     5581 gcaggttaat atcacaaaaa gccgactcat aacagataga tggaacaaca cggatattat
     5641 cactgataaa atttactttt ccataattcc cgctttttga aaaccggtaa ccagccaaac
     5701 cttcgatata atttgaaagg aaggacaata tcttcggata tggtattctt tcggtaaaag
     5761 gaacacattt atcttttgtt ttgatatcaa ttcttttctg cgatgggatt atttgtactg
     5821 ctgcattata ttctgtgaat tctgtttctc cagattcagg tttgcccatc tctccaagaa
     5881 caaatcctgt aatgatctta gtatctgtat tccggtaaag cgtaataatt gaatcaacaa
     5941 ttttatcagt ctccaggcta tcaataaaaa ccatatccgg aaaagcagtt tccggccaga
     6001 tcagataatc tgtatttgga gttaaataaa ttgaagatac cgacagataa tgcttcaaca
     6061 gtttttcatt atccatattg tacttatcag gtattctgat ctgcggatga attgca
//
LOCUS       DAOSPX010000192         6111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11826_length_6111_cov_4.290621, whole genome shotgun sequence.
ACCESSION   DAOSPX010000192 DAOSPX010000000
VERSION     DAOSPX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11826_length_6111_cov_4.290621"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLZ75_09515"
     CDS             <1..528
                     /locus_tag="PLZ75_09515"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOS72669.1"
                     /translation="YGKNIEELLENIQEARAAKRKIILLGVSFALLDLAEKYSPDLSG
                     VIVMETGGMKGRRKEIIREELHAVLKKGFNVDKIHSEYGMTELLSQAYSAGDGLFRCP
                     PWMRILIRDPRDPLCLLEGTGQSGGINIIDLANIHSCSFIASDDLGRMEENGSFEVLG
                     RFDSSDIRGCNLLME"
     gene            complement(555..1766)
                     /locus_tag="PLZ75_09520"
     CDS             complement(555..1766)
                     /locus_tag="PLZ75_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOS72670.1"
                     /translation="MKKQILLLTLSLVLALSVQAQRKDKASASPAPEGYKFTSVIDLK
                     ATPVKNQAATGTCWCFATTSFIESELLRMGKGEYDLSEMFIVRKNYEKRLRDNYLRRG
                     KGNLGPGGLSHDWMNVFTEYGIVPDEVYPGLNYGSPTHNHSELQEFINAVAPVPVQRR
                     NESDQYKKILGSILDTYLGEVPQSFTYRGSTYTPKSFAASLGINSSDYVEITSFTHFP
                     FYTRGILEVPDNWAMERFYNVPLDELIEIMDYAFNQGYTVNWDGDVSEPGFSHQKGYA
                     VMPAGLPAAGGPATDRDRFERSPAQRTGTTSTQEIESPGPEMNISQEIRQAGYENFTT
                     TDDHLMHLTGIVKDQNGTKYYKTKNSWGTERNAFGGYLYMSESYVRAKTLFIMVHKDA
                     IPPAVRTRLGL"
     gene            complement(1780..2145)
                     /locus_tag="PLZ75_09525"
     CDS             complement(1780..2145)
                     /locus_tag="PLZ75_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOS72671.1"
                     /translation="MAESYDLGRKGEEEAAKYLAGKGYRILCRNWKSGRLEVDIIAEN
                     EEFMIFAEVKTRSADCLVDPAGAVTAGKQKSLILAAERYIKRNSIDKDSRFDIISVIR
                     KGCHYRIEHIENAYYPGLK"
     gene            complement(2147..2788)
                     /locus_tag="PLZ75_09530"
     CDS             complement(2147..2788)
                     /locus_tag="PLZ75_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS72672.1"
                     /translation="MEKTSIIIIDDHQLFRNGLKILLNSFDDLEVVGEASNGYEFLKL
                     LPSITADIALMDINMPEMDGIEATRKGLRINPDMRVIALSMYGEEDYYYRMVDAGVKG
                     FLLKDSAISEVRDAIIAVMKGGTYFSQELLHHVIQRIKHREHESKSANLSRREKEILA
                     KICEGLSNQEIADELFISKRTVDKHRANLLGKTNSKNTASLILFAIKNKLIEI"
     gene            complement(2850..3800)
                     /locus_tag="PLZ75_09535"
     CDS             complement(2850..3800)
                     /locus_tag="PLZ75_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOS72673.1"
                     /translation="MVRLIALIISIILQIIAASIAFGFMKMTRYRLSWILLSLSFLLM
                     AVRKFIQLSELLRGTPSYMWQMIDEWLGVVISFMIIIGVVLIREIFYSLKKADIDRSR
                     TEKRVLNAIINTEENERKRFAKDLHDGLGPILSTVKMSLTSLAQRISDPSGTEILKNT
                     NHLVNEAISTIKDISNNLSPHILENFGLSSAIGAFVTKINQTGTVEIDFKSDLENYRL
                     DSDKEVVIYRAVCELINNAIQHSGASKIEIELNKHDKFVTLQFSDNGRGFDTSKLSRE
                     DTKGMGISNIETRVRTVEGVFVLESSPGKGTNALIRVMDW"
     gene            3884..5128
                     /locus_tag="PLZ75_09540"
     CDS             3884..5128
                     /locus_tag="PLZ75_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOS72674.1"
                     /translation="MILLYRLGILFYSVTAMLISPFSSKAKLLVRGRRNSFRVIRDKR
                     IQGDRYVWMHCASLGEFEQGRPVIEAIREKFPEFRILLTFFSPSGYEVRKNYPGADIV
                     CYLPSDTGRNAAKLLDAVKPELVIFVKYEYWKYYLEAVAQRGIPLLLVSALFRREQIF
                     FRWYGAFFRKILENFSHIFVQDEGSAEKLESLGVRNVTVSGDTRFDRVLEIAAAAKSI
                     SQIESFRGSEKLFLAGSSWKPDEEIIVRYINDNPGRMKWVFAPHETDRANIDRLVKLF
                     RVKCVRFSEYGEGEKDARVMIIDNVGMLSSAYRYAYIAAVGGGFGRGIHNILEPATWG
                     VPVLFGPNHEKFREALNLIYLGGAKSFSNYDEFRSVLEDLSENPAFYERSSLAASEYV
                     RENAGATKKIMEWISSEVINSI"
     gene            5335..>6111
                     /locus_tag="PLZ75_09545"
     CDS             5335..>6111
                     /locus_tag="PLZ75_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOS72675.1"
                     /translation="MATVSHGQGSTTSFLQGKITDENQQPMAGATIVATHEPSGTAYG
                     ANTNSQGLYNIEGMRPGGPYSVEISFVGYARRTFTEIYLMLGEHTTLNADLSQTSTEL
                     REVTVIGSRTSKFSTTKTGATTNISGAQMVTLPTVSRNISDITRVSPYTNGMSFSGGD
                     GRSTNFTVDGSNFNNNFGLGSKLPGGGNPISLDAIEEVQVVIAPFDVRQTNFIGGGIN
                     AITKSGTNNLKGSAYTYYTNQKMRGNKIGEHEFSRSDKESN"
BASE COUNT         1539 a         1403 c         1485 g         1684 t
ORIGIN      
        1 tacgggaaga acattgagga gctgctggag aacatacagg aagccagggc cgcgaagagg
       61 aaaataatcc tgctgggtgt cagttttgcc cttcttgatc tggccgaaaa atattcaccc
      121 gatctttcag gggttattgt aatggaaaca ggcggaatga agggcaggag aaaggagata
      181 ataagggaag aactccatgc tgttctgaag aaagggttta acgtggataa aattcattct
      241 gaatacggga tgaccgaact gctgagccag gcatattcag cgggtgacgg ccttttccgc
      301 tgtccgcctt ggatgaggat actcatcagg gatccgcgtg atcctctctg cctgctggaa
      361 ggaacggggc agtcaggcgg aataaatatc attgacctcg caaatattca ttcctgttca
      421 ttcattgcaa gcgacgatct tggcaggatg gaagaaaacg gcagctttga ggtgctcggc
      481 cgttttgaca gcagcgatat ccgcggctgc aatctgctta tggaatagtt tccgaaaacc
      541 cagcagtccg taagctacag tcccagtctg gttctgacag cagggggtat agcatcctta
      601 tgaaccatta tgaaaagtgt cttggcgcgg acatagcttt ccgacatgta aaggtatccg
      661 ccgaatgcat tccgttccgt accccacgag tttttggttt tgtagtattt ggtaccgttc
      721 tggtccttaa cgattccggt gagatgcatc aggtgatcgt cggtggtggt gaagttctca
      781 taaccggcct gcctgatctc ctggcttatg ttcatttccg gacccggaga ttcaatttcc
      841 tgggttgaag tggttccggt tctctgagcc ggacttcttt caaacctgtc cctgtcggtt
      901 gcagggccgc ctgccgccgg caggccggca ggcatcaccg catatccctt ctgatgggaa
      961 aacccgggtt cactgacatc tccgtcccag tttaccgtat aaccctgatt aaaggcatag
     1021 tccattatct caataagttc atcaagggga acattgtaaa aacgttccat tgcccagttg
     1081 tcgggcacct caaggatacc cctggtataa aagggaaaat gggtgaacga tgttatctct
     1141 acatagtctg agctgttgat gcccagggag gcggcaaaac tcttcggggt ataggtactg
     1201 cccctgtagg tgaaggattg gggaacttct cccaggtagg tatccaggat ggaacccagt
     1261 atctttttat actggtcact ttcattcctt ctctgaacgg gaacaggtgc cacggcattg
     1321 atgaactcct gcagttcact gtggttatgt gtcggggatc cgtaattgag gccgggatag
     1381 acttcatccg ggactatgcc gtattcagtg aaaacgttca tccagtcatg cgagagaccg
     1441 ccgggaccga gatttccctt ccccctgcgc aggtaattgt ctctcagccg tttctcgtaa
     1501 ttcttacgta caatgaacat ttcagaaagg tcatattcgc cttttcccat ccggagtaat
     1561 tccgattcta taaatgaagt tgttgcaaag caccagcatg taccggttgc agcctggttc
     1621 ttcacgggag ttgccttcag gtcaatcacg ctggtgaatt tgtatccctc cggggcgggg
     1681 gatgcggatg ctttatcttt cctctgtgcc tgtactgaaa gagcaagaac aagggaaagt
     1741 gtcagaagca ggatttgttt tttcatggaa tcaaagattt tattttaagc ctggatagta
     1801 tgcattttca atgtgctcaa ttctgtaatg acagcctttc ctgattacgg atatgatatc
     1861 gaagcggctg tccttatcga tgctgtttct tttaatatat ctctcggcag ctaatataag
     1921 cgatttttgt tttccggcag ttaccgcacc ggcgggatca acaaggcagt ctgctgatct
     1981 ggtcttcact tcggcgaaaa tcatgaattc ctcattttcc gcaatgatgt caacctccag
     2041 tctgccggat ttccagttgc ggcacagtat cctgtatccc tttccggcaa ggtattttgc
     2101 cgcctcttcc tcgcccttcc ttcccagatc gtacgattcg gccatttcag atctcgataa
     2161 gcttgttttt gatggcgaaa aggatgaggc tggcagtatt ttttgaattt gtttttccga
     2221 gaaggtttgc cctgtgctta tcaacagtcc gcttgcttat aaacaactca tcggctattt
     2281 cctgattgga cagcccctca cagatttttg ccagtatctc cttctctctc cggctgaggt
     2341 tggccgactt gctctcgtgt tcccggtgtt ttatcctctg gatgacatga tgcagcagtt
     2401 cctgtgaaaa gtaagtgcct cccttcatca cggcaataat ggcatcccgc acttctgaaa
     2461 tggctgaatc cttgagaagg aagcccttta cgccggcatc aaccatccgg taataataat
     2521 cctcctcccc gtacatggaa agggcaatta cccgcatgtc agggttgatc ctgaggcctt
     2581 tcctggttgc ctcaatgccg tccatctcgg gcatgttgat atccatcagg gcaatatctg
     2641 ccgttattga gggcagcagt ttcaggaatt catatccgtt tgaggcttcc cccaccactt
     2701 caaggtcgtc aaatgaattg agaaggatct tcaatccgtt ccggaacagc tggtgatcat
     2761 caataatgat tatgcttgtc ttttccatca gtatattact gtgcggcatc agtctgcacc
     2821 atgatgcatt actgacggtt ccgcctgttt taccagtcca ttaccctgat cagtgcgttt
     2881 gtccccttgc cgggagagct ctcaagaaca aaaactcctt caacggttct tacgcgtgtt
     2941 tcgatattcg aaatccccat gcccttcgta tcctccctgc tcagttttga agtgtcaaat
     3001 cctctcccgt tatcggaaaa ctgcaatgta acaaatttat catgtttatt cagctcaatt
     3061 tctatttttg aggctcctga atgctgaatg gcattgttga taagctcaca tacagcgcgg
     3121 taaatcacaa cctccttgtc agagtcaagc cggtagtttt caagatccga cttgaagtct
     3181 atttccaccg tcccggtctg gtttatcttt gtgacaaagg ccccaatcgc gctggaaagc
     3241 ccgaagttct caagaatatg gggactgaga ttgttggata tatcctttat cgtgcttatt
     3301 gcctcattga ccaggtggtt tgtgtttttg aggatctccg tgcctgacgg atcgcttatg
     3361 cgctgggcaa gtgaggtgag tgacattttc accgtggaca gtatcgggcc cagtccgtca
     3421 tgaaggtctt ttgcaaagcg cttcctctca ttctcctcgg tgttgatgat ggcgttcaga
     3481 actctttttt cagtacgcga cctgtcaatg tcggccttct tgagtgaata gaatatctcc
     3541 cttatcagca caactcctat tataatcatg aatgagataa ccacgcccag ccattcatct
     3601 atcatctgcc acatgtagga gggagttccc ctgagcagtt ccgagagctg tatgaatttg
     3661 cgcactgcca taagcagaaa cgacagggaa agaagtatcc atgacagcct gtacctggtc
     3721 attttcatga atccgaaggc tatcgacgct gctattatct gcaatattat tgaaatgata
     3781 agggctatga gtcgtaccat cccggcaatg aggttttaat gcaaaaatag aggtatttat
     3841 ttaattgtac ctttttttta cctttgtcaa ttctgtaaaa gccatgatac ttttatacag
     3901 actgggaata ttattttatt cggtaacagc catgctgatt tcgcccttca gcagcaaggc
     3961 gaaacttctg gtgaggggcc gccgcaactc tttcagggtc ataagggata agcggataca
     4021 gggcgacaga tacgtatgga tgcattgtgc ctcgctcggg gagtttgagc agggcaggcc
     4081 ggttattgag gcaataaggg aaaagtttcc ggaattcagg atactgctta catttttctc
     4141 tccttccggc tatgaagtcc ggaagaatta tcccggggcg gatatagtct gctatctgcc
     4201 ttcagacacc gggcggaatg cggcgaaact tctggatgca gtaaaaccgg aacttgttat
     4261 tttcgtgaaa tacgaatact ggaagtatta cctggaggcc gtagctcaaa ggggaatacc
     4321 gcttctcctt gtttcagctc ttttcaggag ggaacagatc tttttccgct ggtacggggc
     4381 tttcttcagg aaaatactgg aaaacttcag tcatatattt gttcaggatg agggatcggc
     4441 tgaaaagctt gaaagtctgg gtgtcaggaa tgtcacggtt tccggtgaca ccaggtttga
     4501 cagggttctg gagatagcag ccgcggcaaa gtccatttca cagatagaga gcttcagggg
     4561 gagtgaaaaa ctgtttcttg cgggaagcag ctggaagccg gatgaagaaa tcattgtacg
     4621 atatataaat gataatcctg gcagaatgaa gtgggttttt gccccgcatg aaaccgacag
     4681 ggcaaatata gacaggcttg ttaaactttt cagggtgaaa tgtgttagat tttctgagta
     4741 cggtgaaggg gaaaaggacg caagagttat gataattgat aatgtcggca tgctttcgtc
     4801 agcctacagg tatgcatata ttgctgcagt gggcggagga ttcggcaggg gaatacataa
     4861 tatccttgag ccggccacct ggggggttcc cgtattattc gggcccaatc atgaaaaatt
     4921 cagggaagcc ctcaatctga tttacctggg aggggcaaaa tctttcagta attatgatga
     4981 gttcaggtca gttcttgaag acctttcaga aaatccggca ttttatgaaa gatcttccct
     5041 tgcagcttcg gaatatgtga gagaaaatgc gggagccaca aagaaaatca tggaatggat
     5101 cagcagtgaa gttattaaca gcatatgatg aaaacttgtt tataacctgt tggcaattac
     5161 cttgtgcata tagttaaaag ggccgtaaat tcggctttct cccgatttgc gggctgtttt
     5221 ttggaaaaaa gtgaaaccgg ttaataataa ttatgttaca gtattaaacc taaaccagaa
     5281 tgagatatga acaagctttt aaaactcttt tattccgcgc tgctcttcct gttcatggca
     5341 accgtcagcc acggacaggg atcaacaact tcctttcttc agggaaagat aactgatgag
     5401 aatcagcaac ccatggccgg ggcgaccatt gttgcaaccc atgagccctc tggaaccgct
     5461 tacggtgcca acaccaacag tcagggcctc tacaacattg agggaatgcg tccgggagga
     5521 ccctatagtg ttgagatcag ttttgtgggc tatgccagga gaacattcac tgaaatatat
     5581 ctgatgctcg gagaacatac caccctcaat gccgacctgt cccagacaag tacggaactc
     5641 cgggaggtga cagtcatagg cagcaggact tcaaagttca gcaccacgaa aaccggtgct
     5701 accacgaata tttcaggcgc acagatggta actcttccaa ctgtctcccg caatatcagc
     5761 gatatcaccc gggtatcccc ctataccaac gggatgagct tttccggagg tgacggacgt
     5821 tcaacgaatt ttaccgttga tggttcaaat ttcaacaaca acttcggact gggaagcaaa
     5881 cttcctggcg gtggcaatcc catttcgctt gatgccatag aggaagtaca ggttgttatt
     5941 gcccctttcg atgtgaggca gaccaatttc atcggaggcg gaatcaatgc gatcaccaaa
     6001 tcgggtacca ataacctgaa aggttcagcc tatacctatt atacaaacca gaaaatgcgc
     6061 ggcaacaaga tcggcgagca cgagttcagc aggagcgaca aggaatcaaa t
//
LOCUS       DAOSPX010000193         6100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11858_length_6100_cov_4.724069, whole genome shotgun sequence.
ACCESSION   DAOSPX010000193 DAOSPX010000000
VERSION     DAOSPX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11858_length_6100_cov_4.724069"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..886
                     /locus_tag="PLZ75_09550"
     CDS             320..886
                     /locus_tag="PLZ75_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72676.1"
                     /translation="MSKLFFISLSVFITALLTGGCRKDSILSREQEVLFQYEYRNDAE
                     GKRLEGFFIDRQGNVLTYRNPAEWNHPDQDLSIYSDQLSVNLDKCSVSEKHVSAAELE
                     KYSRLISNISSSKVSAARHSGTGAGTHRFICYSFDEATKAYKGFLIRAEGEISRENLN
                     FYSKKIVAWMKDVNRDITDYQSQLSGPQ"
     gene            complement(933..3302)
                     /locus_tag="PLZ75_09555"
     CDS             complement(933..3302)
                     /locus_tag="PLZ75_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOS72677.1"
                     /translation="MILTERVKRLRERSLKAVETLSAERARLLTCFYKSDEARELSAP
                     VRRAKAFEFILRNKSLYIGEDELIVGERGPAPKKTPTYPEISLHSGEDLEILNSRKKI
                     SFRVSDETRNIYRKEIIPYWKGKSNRDRIMSLMSPEWHKAYEAGIFTEFQEQRAPGHT
                     VLGYRMFRMGFLDIKNEIRASMADLDYYHDREAYRRLEELKAMDIACDALIMYACRYA
                     AALSELAEKEKDAVRKGELEKMAAVCRKVPARAPGTVHEMLQHYWFIHLGVITELNPW
                     DSFNPGRLDQHLWPVYKKQKEEGSLSDEELYELLGCFWIKFNNHPAPPKMGVTASESS
                     TYTDFCLVNLGGVKPDGTDAVNEMTYILLDVIREMRLLQPGSMVQISRKNPDRFIHRA
                     LDIIRTGFGQPSCFNTEAIIQELLRQGKSIEDARNGGASGCVETGAFGTEAYWLTGYF
                     NLPKILELTLNNGYDRRTEAQAGLKTGYAGDYKTFEELLAAFRTQVNYLADIKIRGNN
                     IIQRTFAEWLPAPFLSVLIEDCIRKGTDYNDGGARYNTTYIQGVGLGSIADMLTSIRY
                     NIYESGRISWKEMNEALNADFEGFEELQHDLIHDTPKYGNDDDYADRQAVSVFEIFYD
                     AVCERPDARGGMHRINMLPTTCHVYFGSVTGATPDGRKAGRPLSEGISPAQGADREGP
                     TAVIKSASKIDHLRTGGTLLNQKFIPRFFEGTDSYDCLTALIRSYFALDGHHVQFNVV
                     SASTLRDAQKHPELYRGLIVRVAGYSDYFNDLGEDLQNEIIMRTEHGST"
     gene            complement(3344..4144)
                     /locus_tag="PLZ75_09560"
     CDS             complement(3344..4144)
                     /locus_tag="PLZ75_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOS72678.1"
                     /translation="MKGLIFSVKRYSVHDGPGIRVTFFMKGCPLACKWCHNPEGISPL
                     PENMERVSRIGAGEIRRQEKIGKYYQIEDVLRILEKESIFIGQSGGGITFSGGEPLFQ
                     PDFLLEALKACRAGGYHTAVDTSAYSPARDFRAIIPYTNLFLFDIKHLDDSRHFEFTG
                     VSNAGIIENFDTLLQEGADIYARIPVIPGFNDSQDHLRRLKAFLNDRKSSSLKMICLL
                     PYHRPGSSKYGKLGIPLEMNGLKPPSEKRMNELKEYFSDLGIRVKTGG"
     gene            4754..>6100
                     /locus_tag="PLZ75_09565"
     CDS             4754..>6100
                     /locus_tag="PLZ75_09565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOS72679.1"
                     /translation="MKNDNPFFSKWDTPYEVPPFDKIKAGHFMPAYLKAFEEQKKEIS
                     AIINNKKEPSFDNTIRALEYSGQLLTKVSRVFGALNSAHTNDSLQAINMEIAPLLSRH
                     RDDINLNDSLFQRVRAVYDNMDKFDLTEEEQKVLDNTYKNFVRSGAALSPEDKEKLRK
                     INEEHSLLTVKFGQNLLAETNDFILVIDKREDLAGLPVSVIDQAAARARGMNMDGKWV
                     FTIQYPIMEPFLKYSEKRDLRKKLFTAYFMKGDNDNERDNKEIIREIARLRVERSRLL
                     GYDSYAHFVLERNMAKTPERVFDFLNEIWAAALPVAKAEAAAQQEIINREGGRFRLEP
                     WDWWYYSEKIKKERYDLDDELTRPYFQIENVIGGMFHVANKLYRLDFSPRNDIPKYHP
                     DVRTYEVTRDGKHVGILMIDNYPRPSKRSGAWCGAYRGQSRDIRGRMITPVVTMVTN"
BASE COUNT         1670 a         1455 c         1320 g         1655 t
ORIGIN      
        1 tgctcagtac atcctgcctg tgcctgatat caaaataatc aaagaatttt tccatgtttc
       61 tcttttttcc gtttgagaca attcattgca gcactgatcc tgcctgacta attatgtgat
      121 gcttatccat gagttacaac tgtccggctt atgcacccgg cagcccatcc attcataaat
      181 ataaatcttc aataatataa aaaataatat tgaattacta aaataatttt aattttcgta
      241 gggggcaatt gttgaaaaat atgcttctgt tcacgctaat catgatgata atggtccggt
      301 ttttgctgaa ctaatgatca tgtcaaaact gttcttcata tcactgtctg tgttcattac
      361 agctcttctc acgggcggat gcaggaagga ttccattctg agcagggaac aggaagtgct
      421 tttccagtat gaataccgca atgatgcgga gggaaagcgt ctggaaggat tcttcattga
      481 caggcagggc aatgttttaa cttacaggaa tccggctgaa tggaatcacc cggaccagga
      541 cctgtcaata tacagcgacc agctttcggt gaatctggat aaatgttctg tttcagagaa
      601 acatgtttca gcggctgaac tggaaaaata ttccaggctt atttccaata tctcttcaag
      661 caaggtatct gcagcacgtc attcaggaac aggtgcaggt acccacaggt ttatatgcta
      721 cagttttgac gaagcgacaa aggcatacaa gggattttta atcagggcgg aaggggaaat
      781 aagccgcgaa aaccttaatt tctactcgaa aaaaatcgtt gcctggatga aggatgtcaa
      841 ccgcgacata acggattatc agtcacagct gtccggacct cagtaatccc accagtttca
      901 gtaatccctc caatccttcg cctgatcacc tgtcaggtgc tgccgtgttc agtcctcatt
      961 attatttcat tctgcaggtc ctcgcccaga tcattgaaat aatcgctgta tccggcaacc
     1021 cttacaatca ggccccggta cagttccgga tgtttctgtg catccctcag tgttgaagca
     1081 ctgaccacat tgaactgtac atgatggcca tccagtgcaa aatagctgcg gataagcgct
     1141 gtaaggcaat catagctgtc agtgccttca aaaaaccggg gaatgaattt ctgattcagc
     1201 aatgtacccc ctgtccgcaa atgatctatt tttgaagctg actttataac cgcggtaggc
     1261 ccttccctgt cggcgccctg tgcgggagat attccctccg acagagggcg gcccgctttg
     1321 cggccgtcag gtgttgctcc cgtcacactg ccgaaatata catgacaggt tgtcggaagc
     1381 atgttgatcc tgtgcattcc gccccgtgca tcaggtcttt cacagaccgc atcataaaag
     1441 atctcaaaaa ccgatactgc ctgccggtcg gcataatcat catcatttcc gtatttcggg
     1501 gtgtcatgaa taagatcatg ctgcaattct tcaaatccct caaaatcggc attcagagcc
     1561 tcgttcatct ccttccagga aattcttccg ctttcataaa tattgtacct tattgatgtc
     1621 agcatgtcgg ctatgcttcc aagtcccaca ccctggatat atgtggtgtt gtatctggca
     1681 ccgccgtcat tgtagtctgt gccctttctg atgcaatcct caatgagaac tgacagaaag
     1741 ggtgcgggaa gccattcggc aaatgtccgc tgaataatgt tgttgcccct tatttttatg
     1801 tctgccagat agtttacctg agtcctgaaa gctgccagga gttcttcaaa ggtcttgtaa
     1861 tccccggcat accctgtctt aagtccggcc tgggcttccg ttctcctgtc ataaccgttg
     1921 ttcagggtca gttccagaat cttgggaaga ttgaaatagc cggtcagcca gtatgcctca
     1981 gttccgaaag ctcccgtttc aacacagccc gaggctcctc cgttacgggc atcctcaatg
     2041 cttttcccct ggcgcagcaa ttcctggatt attgcttccg tattaaagca ggacggctgg
     2101 ccgaaaccgg tacggattat atccagtgcc ctgtgaatga acctgtcagg attcttgcgg
     2161 cttatctgca ccattgagcc gggctgaagc aatctcattt ccctgatcac atccagaagg
     2221 atgtatgtca tttcgttaac agcatccgtg ccgtccggct tcacgccacc caggtttacc
     2281 aggcagaaat cggtgtaggt actgctttca gaagcggtaa cacccatttt aggaggagcc
     2341 ggatgattat tgaattttat ccagaagcag ccaagaagct cataaagctc ttcatcactc
     2401 aatgatccct cttccttttg ctttttgtaa accggccaca gatgctgatc cagccggccg
     2461 ggattgaagg aatcccaggg attgagctcc gtgattaccc caagatggat aaaccaatag
     2521 tgctgaagca tttcatgcac ggtccctggt gcccgggcag gtactttccg gcaaacagca
     2581 gccatttttt caagttcccc cttcctgacg gcatccttct ccttttccgc aagttcggaa
     2641 agtgcggcgg cataacggca ggcatacatg ataagcgcat cacatgcaat atccatagcc
     2701 ttcagttctt ccagccgccg gtaagcttcc ctgtcatgat aataatcaag atccgccatg
     2761 cttgccctta tttcattctt aatatccagg aaacccatgc gaaacatcct gtaacccaaa
     2821 acagtatgac ccggagccct ctgttcctgg aattcggtga aaattcctgc ctcataggct
     2881 ttatgccatt caggactcat caggctcatt atcctgtcac gattgctctt tcccttccag
     2941 taaggaataa tttcctttct gtaaatattt cttgtctcgt cactgaccct gaaggagatc
     3001 ttttttcttg aattcagtat ttcaagatcc tccccggaat gcagacttat ctccggatag
     3061 gtgggagttt tcttcggggc cggtcccctc tctcccacta taagttcatc ctcccctata
     3121 tacaggctct tattccggag tatgaactcg aatgcctttg cccttctgac cggagccgac
     3181 aactcccggg cctcatcact tttataaaaa catgtcagga gccgggccct ttcggcagac
     3241 aatgtttcca ccgccttaag gctcctttct ctgagtcttt ttactctttc tgtcagaatc
     3301 atatacacgc tgatttatta atttcacccg atgtcttatc tgattatccc ccggttttga
     3361 ccctgatgcc cagatcactg aagtattcct tcagctcatt cattcttttc tctgaaggtg
     3421 gtttcaaacc attcatttcc aggggaattc caagcttacc gtattttgat gaaccaggcc
     3481 tgtgataggg aagcaggcag atcatcttca ggctgctgct tttccggtcg ttcagaaaag
     3541 cctttaatct ccgcagatgg tcctgactgt cattaaaccc tggtattacc ggtattcttg
     3601 cataaatgtc cgcgccttcc tgcagcaggg tatcaaagtt ctcaataatg ccggcattcg
     3661 atacccccgt gaattcaaaa tgcctggaat catcaagatg ctttatgtca aagagaaaga
     3721 gatttgtata gggtattatt gccctgaaat cccgcgcagg ggaataagcg gatgtatcga
     3781 cggcagtatg atatcctcct gccctgcagg ctttcaaagc ttccaggagg aaatcaggct
     3841 gaaagagggg ttcgcctccc gagaaagtta ttcctccccc tgactggccg ataaaaatac
     3901 tttccttttc caggatacgg agaacatcct caatctgata atactttccg attttttcct
     3961 gccgccttat ttccccggct ccaatcctgc tgactctctc catgttttcc ggaagcgggg
     4021 atatgccttc cgggttatgg caccatttgc atgcaagagg acagcctttc atgaaaaatg
     4081 ttaccctgat cccgggtccg tcgtgaactg aatatcgctt aacgctgaaa attaagccct
     4141 tcattgcaac aaaatgttat ttgtatggta taatgctgaa aaggaaaact tgttccggag
     4201 ctacaacagc tgaaatcaaa tcagctgtca tagaacataa gacagcaata cagtctccgg
     4261 ccgaggcgct gcctgttgaa acgatattcg agccctgata acatatgaat gcaagttatg
     4321 tatttttaca taataaaaaa acagtgtcaa ttcatatcgg ttttcccgtg gattcgggcc
     4381 aatacttaag aatttttaac aatgattaac attacattaa cgtttatctt tgcaacaaat
     4441 ttggttactt tgcgtcatac atattgaaaa gtctattcat aggccgttcg gccaattcta
     4501 cgatcaggtt tatagttaat cgcggaaaga caccgcccgg tgtctttcct cttttttaca
     4561 gccccttatc agttataaaa acattattcc ctttctttgc atcaaatcaa ttttgtattt
     4621 tacatccccg ggaaaaaacg ggaattaatg gcagggaata tcatatcaaa aaaaaataaa
     4681 gcaaagtaaa atgaaaaggt taatactaat aattgccgca gcagcaattg gattcacagg
     4741 atgtaaaaca aagatgaaaa acgataatcc tttcttcagc aaatgggata ctccgtatga
     4801 ggtccccccc ttcgataaaa ttaaggcagg ccatttcatg cctgcatatc tgaaagcatt
     4861 cgaggaacag aaaaaagaaa tcagtgcgat aataaacaat aaaaaggaac cctcctttga
     4921 caatacgatc agggccctgg aatacagcgg ccagcttctt acgaaagtca gcagggtttt
     4981 cggggcactg aattcagcgc atacaaacga cagccttcag gcaataaata tggaaatagc
     5041 tcccctgctg tccaggcacc gggatgacat aaatctgaat gacagcctgt ttcagagagt
     5101 cagggcggtt tatgacaata tggacaagtt tgatcttacc gaagaagagc agaaggtgct
     5161 tgacaatacc tacaaaaatt ttgtaagaag cggtgcggca ttatcccctg aagacaagga
     5221 gaagctgaga aaaatcaatg aggagcattc actgcttacg gtgaagttcg ggcagaatct
     5281 ccttgccgaa acaaatgatt tcatactggt gattgacaaa agggaagatc ttgcaggact
     5341 gccggtgagt gtcattgatc aggcagcagc aagggcaagg ggaatgaata tggatggcaa
     5401 atgggtattt acaatacaat atcccatcat ggagcccttc cttaaatatt ccgaaaaacg
     5461 cgatctgaga aaaaaactgt ttacggcata tttcatgaag ggagacaatg acaatgaaag
     5521 ggataacaag gagattatca gggaaattgc cagactgaga gttgaaaggt ccagactgct
     5581 tggctatgac agctatgccc attttgtact ggaaagaaat atggccaaaa ctcctgaaag
     5641 ggtatttgat ttcctcaatg aaatatgggc ggcggcatta cctgtggcaa aggcggaagc
     5701 tgctgcacag caggagataa tcaatcgcga gggaggcaga ttccggcttg aaccatggga
     5761 ctggtggtat tacagtgaaa aaatcaaaaa ggaaaggtac gatcttgatg atgaactgac
     5821 acggccatat ttccagatcg aaaatgttat aggaggaatg ttccatgtgg caaacaaact
     5881 ttacaggctc gatttctcgc cacgtaatga cattccgaaa tatcatcccg atgtcagaac
     5941 gtatgaagtt acacgcgacg gaaagcatgt gggtattctg atgatagaca actacccgcg
     6001 tccttccaag cgcagcgggg catggtgcgg ggcataccgc ggtcagagca gggatatcag
     6061 gggcaggatg atcacccccg ttgtcaccat ggtgacaaac
//
LOCUS       DAOSPX010000194         6064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11933_length_6064_cov_4.647362, whole genome shotgun sequence.
ACCESSION   DAOSPX010000194 DAOSPX010000000
VERSION     DAOSPX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11933_length_6064_cov_4.647362"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1244)
                     /locus_tag="PLZ75_09570"
     CDS             complement(15..1244)
                     /locus_tag="PLZ75_09570"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS72680.1"
                     /translation="MKRRKLIKNLTILPLSGAVAGSTLTFGTVATACSSSKAKRDIFR
                     ELGIRTFINAAGTYTALSASLMHEEVKDAWLASSEEYAVLDEVQDKVGERIAEMCHAE
                     AATVTAGCFSALVLGMAGVLTGNDTKKVAQLPDLTGMKSEVLTQKSHNWGYIHALKLT
                     GIKLVEVETVQDVEKAVNENTAMMWFLNDAALSGQIGAEEWVALGKKFNIPTMNDIAA
                     DVPPVENLWKYNDMGFDLVCISGGKGLCGPQSAGILMGRKDLIEAARLSAPPRSSTIG
                     RGMKVNKEEIIGMYVALDKYINQDHEAEWKMWEDRVAVIESAAREVEGVTTEVSVPPI
                     ANHTPTLTISWDIRKINVSGRDFIIRLQNGNPSIEVRGGRNESIVITVFMLKPGQDKI
                     VAKRVREELIKASVSQS"
     gene            complement(1337..2617)
                     /locus_tag="PLZ75_09575"
     CDS             complement(1337..2617)
                     /locus_tag="PLZ75_09575"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HOS72681.1"
                     /translation="MQNKKITPGRIYGCIHILFFAAFMAIGSVMNAQSVDILLKGGHV
                     IDPKNNIDSRMDVAIAGGKIVQVAPNIPSNTAKRTVDVSGMFVTPGLIDIHVHVFHGM
                     DPSYIANGYTSLPPDGFTFRAGVTTVVDAGSAGYRNFELFKEQTIDRSETRVLAFLNI
                     VGEGMRGGPLEQNIKDMNAHRAVAMARKYPDEIVGFKLAHYEGYDWRPTDSTVKAGTL
                     TGMPSMIDFGVSNPPLPLEELFMKHLNPGDIFTHCYAYFPKTRDAIVDENGKVKPFVF
                     AARKRGIIFDVGHGGGSFRWNQAVPAIQQGLIADALSSDLHTGSMNSGYKDMANLMSK
                     FMALGLSVQDVVARSTWYPAKIINRPELGNLSVGSEADVAVFSLVKGNFGFVDINREK
                     MMGTQKLIAELTLRAGKIVYDLNGITSPLYRAGK"
     gene            2829..4181
                     /locus_tag="PLZ75_09580"
     CDS             2829..4181
                     /locus_tag="PLZ75_09580"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOS72682.1"
                     /translation="MKRDVFSRRAFLTKGGIGGLGLFTIGSAMACSRSTDKSGTIESG
                     RGKIENIGGISLEQLRDQYRSELYDYFIPNMDKYAVDHKYGGVICSLDIRTGEPENMN
                     KTAWFVGRGLWVYSYLYNNLEPDSRFLEIARKSKDLLLKLQPKDDSFWPETFSREGKP
                     LSGPGDIYGSLFVAEGLSEYAEASGEKEYRDLAKKIIFDCVARYDQPDYSYSIGSYLK
                     NSPPVQGPRILGHWMILLSLSTQMLMHEPDEDLEKLAARCVDAILNHHVNAEYGLTNE
                     VLNHDFSLPDNEYAQHSITGHGLETLAFVMFEAGRLRDRNLFRSAEKVFKKIVDVCAD
                     HVYGGYFHTLQQVDNYIWSTGKSLWCQQEVINGALFLIEHTGDEWAMRHFERANSHIF
                     EKYFNPAHKFWHFGGDRKMVNRNVSLMEHYHHARQFVRGLLTFERLIERGGRISDLFA
                     "
     gene            4269..5525
                     /locus_tag="PLZ75_09585"
     CDS             4269..5525
                     /locus_tag="PLZ75_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72683.1"
                     /translation="METRYTENRTLILIVILLSLMCSCQPGDKGIRVVFPDVSEAVQV
                     TGNDREHLLASYYGINSWSRDQRYITVLETDIKYKLPDETEPAVLGLVDLKNNNFIPL
                     TETRAWNFQQGCMAHWLATSPDSLIIFNDFRNGKFVSVIMNVHKKQEIRTIPHPVSAV
                     SPNGREAVSINFSRLRLTRPDYGYGGNGQDPQADIPLPDNDGLFLVDLETGESGLLVS
                     LEQVKDLIPPVSEGSIGWFNHTLFSRDGSKIFWLSRQMEGSRRRTTSLTVNRDGTDLR
                     RCFPDNWEGSHFDWLNDDELMVTASYEGKQYAHVLFTVGKQDYKRLGGGLLDYDGHGT
                     FSPDQKWMVTDTYAGSGMREHKIYLMDMESEAVISLGRFVQPPEFDGYWRCDLHCRWS
                     PDGSMLGFNSTFTGSRQVYIMRLDYL"
     gene            5555..>6064
                     /locus_tag="PLZ75_09590"
     CDS             5555..>6064
                     /locus_tag="PLZ75_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS72684.1"
                     /translation="MKNKILLFSLVLILISGFPGVSIYAALNVTGQDTLTFIHVSDVH
                     ICNLKGYHPEFVQRRQHFGDGMSPFREFLATVPAKVRADMVIVTGDNVDYYEAETESG
                     GMLDTQIEQYVRLIDNSQVPVFLTLGNHDIASYRITPGPWSGNNQLEAGRARATWMRN
                     VPCFKDGTYY"
BASE COUNT         1591 a         1381 c         1407 g         1685 t
ORIGIN      
        1 tgaagaataa tttcctagct ctgacttacg ctggctttga tcagctcttc ccgcaccctt
       61 ttggctacga tcttatcctg tccgggtttc agcatgaata cggttattac aatactttca
      121 ttcctgcccc cgcgtacttc aatggaggga ttcccgttct gaagcctgat aataaaatcc
      181 ctcccgctta cattgatctt gcggatatcc catgaaattg tcagtgttgg agtatggttg
      241 gcaatgggag gtacgctgac ctctgtggtg acaccttcca cttcccttgc cgcactttca
      301 atcacagcaa ccctgtcttc ccacatcttc cattcagctt cgtgatcctg gttaatatat
      361 ttatccagtg ccacatacat acctataatt tcttccttgt tcactttcat gccacggccg
      421 atagtactgg agcgcggagg tgcactcaga cgtgctgctt caatcagatc ctttctaccc
      481 atcaggatac ctgcactttg cggaccacac aggcccttac cgcctgaaat acaaaccaga
      541 tcaaagccca tgtcgttata tttccagaga ttctcgacgg gaggtacatc tgccgctata
      601 tcattcattg tcggtatgtt gaactttttg ccaagggcaa cccactcctc agcaccgatc
      661 tgtccgctaa gggcagcatc attgagaaac cacatcattg cagtgttttc gttaacggcc
      721 ttttcaacgt cctgtactgt ttcaacctca acaagtttga tgccggtgag cttcagtgca
      781 tgaatgtatc cccagttatg tgatttctgt gtcagcactt ccgatttcat gccagtaaga
      841 tcggggagct gggcaacttt ttttgtatca tttccggtta gaacgccggc cattcccagt
      901 acaagggcag agaaacatcc tgctgttacc gttgcggctt cagcatgaca catttcggca
      961 atcctttctc ccaccttatc ctgaacttca tccagtactg catattcctc agatgaagca
     1021 agccatgcat ccttaacttc ttcatgcatc agggaagccg atagtgccgt gtaggttccg
     1081 gctgcattga tgaaggtccg tattccgagt tccctgaaaa tatcgcgttt tgcttttgag
     1141 gatgaacatg ctgtagccac tgttccgaaa gtaagagtac ttccggctac tgcccccgac
     1201 aagggcagaa tcgtaaggtt tttaatcagt ttcctgcgtt tcatttttac tgtatttttt
     1261 caattaggtt ttttatcagt ggtgattttt aaaagctgcc ctgaaatgaa tatcatttca
     1321 tggcggcttg atccgattat tttcctgctc tgtacagggg acttgtaatt ccgttcaggt
     1381 catagacgat tttcccggca cggagggtta attccgcaat cagcttttgt gttcccatca
     1441 tcttttccct gtttatgtca acaaatccaa aattcccctt taccaggctg aatacagcga
     1501 catcagcctc tgagccgaca ctcaggtttc caagttccgg tctgttaatg atctttgccg
     1561 ggtaccatgt ggaacgcgct acaacgtcct gaacggacaa tcccagtgcc atgaacttgg
     1621 acatcaggtt ggccatgtcc ttgtatccgc tgttcatact tcccgtatga agatcagaac
     1681 tcagggcgtc ggctattaac ccctgctgaa ttgccggaac ggcctggttc cagcggaagg
     1741 atcctcctcc gtgtccgacg tcaaatatta ttcctctttt ccgagccgca aaaacgaaag
     1801 gcttaacttt tccgttctcg tccacgattg cgtcgcgtgt tttgggaaaa taagcatagc
     1861 aatgtgtgaa gatgtcaccc ggatttaaat gcttcatgaa caattcctca aggggcagag
     1921 ggggattact aacaccaaag tctatcattg acggcattcc tgtaagcgtt cctgccttta
     1981 cagtactgtc tgtgggtctc cagtcataac cctcgtaatg ggccagcttg aatcctacaa
     2041 tctcatccgg gtatttcctg gccattgcaa cggccctgtg ggcattcatg tccttgatat
     2101 tctgctccag aggacctccc ctcatacctt cacccacaat attcaggaaa gcaagaaccc
     2161 tggtttctga cctgtctata gtctgttcct tgaaaagttc aaaattcctg tatcctgccg
     2221 agcctgcatc aacaactgtt gttacccctg ccctgaaagt aaagccgtca ggcggaagtg
     2281 aggtatatcc gttggctata taggatggat ccatcccatg aaacacatgg acatgaatat
     2341 ctatcagtcc gggtgttacg aacattccgc tcacatcaac ggtcctttta gccgtatttg
     2401 aaggaatatt tggtgctacc tgaacaattt tgcctccggc tatcgccaca tccattctgg
     2461 aatcaatatt atttttcgga tcaattacat gtccgccttt caacaggata tcgacggact
     2521 gggcattcat aactgaaccg attgccatga aagccgcaaa aaacagaata tgaatacatc
     2581 cgtatattct tcccggtgta atttttttat tttgcattac tggttttttt aaaagattaa
     2641 aaactattaa ttattcaatt ctacgaagat aaaaaagttc tttgaaactg aataaagcct
     2701 gctatattag gggcctgaag aataaagaac tgaatcaaaa ccggcaaagt tgcctcattc
     2761 actttatttc aatgattctg aggcaaactt ataaagcctg taaaatcaat aagttgtatt
     2821 atatcaagat gaaaagagat gttttttcaa gacgtgcttt cttaacgaaa ggaggcatcg
     2881 gaggattagg actgtttaca attggaagcg ccatggcctg cagccgcagt actgataagt
     2941 ccggtactat tgaaagcggg cggggaaaga ttgaaaatat cggcggaata tcccttgaac
     3001 agcttcgcga tcagtacagg tcggagctgt atgattattt tattcccaat atggacaagt
     3061 atgccgttga ccataaatat ggcggtgtga tttgttctct ggatatccgt acaggtgaac
     3121 cggaaaatat gaataaaaca gcctggtttg tcggcagggg cctttgggta tattcatatt
     3181 tatacaataa cctggaacca gattcccgct ttctggagat tgcacgcaaa tcgaaggatc
     3241 tgttgctgaa gttgcagcca aaagatgaca gtttctggcc ggaaactttc agccgtgagg
     3301 gaaagcctct ttcaggaccg ggtgatattt acggaagcct gtttgttgct gaaggtttgt
     3361 ctgaatacgc tgaagcatca ggagagaaag aataccggga cctggcaaag aaaattattt
     3421 ttgattgtgt tgcccgctat gaccagcccg attacagtta cagtatcggg agctatctca
     3481 aaaattcccc gccggtacag gggcccagga tattgggtca ctggatgata cttctcagtc
     3541 tgtcaactca gatgctgatg catgaacctg atgaagacct ggaaaagctt gccgcacgct
     3601 gtgtggatgc catactgaat catcatgtaa atgcagaata cggacttacc aatgaggttc
     3661 tcaaccatga tttcagtctg ccggacaacg aatatgcaca acattcgatt accggccatg
     3721 gtctggagac actggctttt gtgatgttcg aggccggcag gctgagggac aggaatctgt
     3781 ttcgttcggc tgaaaaagta ttcaagaaaa ttgtggatgt ctgtgctgat catgtatatg
     3841 gcggttactt tcacaccttg cagcaggtgg ataactacat atggagtaca ggcaagtcac
     3901 tgtggtgcca gcaggaagtc ataaacggag ccctgtttct gattgagcac acgggtgatg
     3961 agtgggcgat gcgtcatttt gaaagagcaa acagtcatat ttttgaaaaa tacttcaatc
     4021 cggcgcataa attctggcat ttcggaggag accggaagat ggtgaaccgt aatgtaagcc
     4081 tgatggaaca ttatcatcat gcccggcagt ttgtcagggg attgcttaca tttgaacgcc
     4141 tgatagaacg cggaggcagg atatcggatt tgtttgcctg aaacaaaaag tgcccttgcc
     4201 gcggaaagat acccggtgcg gaacttcacc gatcagggct gttttaaatc atataaattt
     4261 ttgcagatat ggaaacaagg tacactgaaa acaggacatt gatactgatc gttatcctcc
     4321 tgagccttat gtgttcgtgt caacccgggg acaaggggat acgagtggtt tttccggatg
     4381 tgagcgaagc tgttcaggtt acaggaaatg acagggaaca tctgcttgca agttactacg
     4441 gaataaattc gtggagcagg gatcagagat atatcacggt gctggagacg gacataaaat
     4501 ataaacttcc ggatgagacg gaaccggcag ttctgggact tgttgacctg aagaacaata
     4561 attttattcc gcttacggaa acccgggcct ggaatttcca gcagggatgc atggcacact
     4621 ggctggcaac atcgccggat tcacttatta ttttcaatga tttcaggaac ggaaaatttg
     4681 tttctgtaat aatgaatgtt cacaagaagc aggaaatcag gacaattccc caccctgtaa
     4741 gcgctgtttc tcccaatggc agggaagcag tcagcatcaa tttttccagg ctcagattaa
     4801 cccgtccgga ttacggctac ggagggaacg gacaggatcc acaggcagac atacccttgc
     4861 cggacaatga cgggctgttt cttgtagatc ttgaaacggg tgagtccgga ctgcttgttt
     4921 cactggagca ggtaaaagat cttattcctc ctgtaagcga aggttccatc ggctggttta
     4981 atcatactct cttcagcagg gatggttcaa aaatcttctg gctttcacgt cagatggaag
     5041 gttcaagacg cagaacaact tcccttacgg ttaacaggga cggtactgat ctgaggagat
     5101 gctttccgga taactgggaa ggatcccatt tcgactggct gaatgatgat gaacttatgg
     5161 tcactgccag ttatgaaggg aaacaatatg cacacgtact gttcacggtc ggaaaacagg
     5221 attataagag actgggaggg ggactgcttg attatgacgg gcacggtacc ttctcaccgg
     5281 accagaagtg gatggttact gacacttatg caggttcagg catgagggaa cacaagatct
     5341 atctgatgga tatggagagt gaagcggtta tttccctggg aaggtttgtt cagccacctg
     5401 aatttgacgg atactggcgt tgtgaccttc attgcaggtg gagccccgac ggcagcatgc
     5461 tgggattcaa ttcaacattt acaggcagca gacaggtcta tatcatgaga ttggattatc
     5521 tttgaaagta taagcaaatc caaatacctg aaccatgaaa aataaaatcc ttcttttcag
     5581 ccttgtcctt attctgattt caggctttcc cggtgtttcc atttatgctg ccttaaatgt
     5641 aaccgggcag gatactctta cattcattca tgtcagcgat gtccatatct gcaatctgaa
     5701 aggctatcac cctgaatttg tacagaggcg ccagcatttc ggggacggga tgagtccttt
     5761 ccgggaattt cttgccactg tcccggcaaa ggtgagagcc gacatggtca tagtaaccgg
     5821 tgataatgtg gattattatg aagcagaaac tgaaagcggc ggaatgcttg atacccagat
     5881 tgagcaatat gtccgtctga ttgacaacag tcaggttcct gttttcctta ccctcggaaa
     5941 tcatgatatt gcaagctaca ggattactcc cggtccgtgg agcggaaaca accagcttga
     6001 agccggaagg gcgcgtgcca catggatgag gaatgttccc tgctttaaag acggcaccta
     6061 ttac
//
LOCUS       DAOSPX010000195         6064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_11934_length_6064_cov_4.467798, whole genome shotgun sequence.
ACCESSION   DAOSPX010000195 DAOSPX010000000
VERSION     DAOSPX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11934_length_6064_cov_4.467798"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4612)
                     /locus_tag="PLZ75_09595"
     CDS             complement(<1..4612)
                     /locus_tag="PLZ75_09595"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOS72685.1"
                     /translation="MKFSRFFIATIFIAIAVSCGRGDKSSQKQELPKVPGLTGTVLPL
                     DNGFSRYISAYTSGIIPAASAIEIRFTQEFASAAGKRLPSGLFEFSPAVKGKTEWADE
                     TTLVFRPSKPLDPGKTYTGRLNIHKLGNVEERLKEFPIRIQTLKKDFTISCGIPESSP
                     EGDRYTITGELTTADFIPDAEAESFVDARLNRRKTVIKWEHEDNLVHRFTLTDIVREE
                     RAGELTISWDGTVSGIRRKGSQNISIPASGDFIVMDVRTRSGDNPAIDIVFSDPVDES
                     QETEGLVFLEPAMRFTTSISRNVLSLLPADRLPQTVTLNIEPSLRNIKGKTLSSSFSS
                     KLEFAPLRPEIRLIGKGLIVPSSQNLIFPFRAANLKAVDLKIIKIFENNLPYFLQESE
                     MDRGYYIKRFGRPVYSGRVDLNPESGSGGLASTGTGNPAAGNSPGTGTENSGAITAAG
                     TGSWNLYTIDLADYINVEPGVLYNVRLSMRRSYSTLSCSGDGRPSRFEELLDEAREME
                     RSYYDDPDDYFDDSHESIYYSMGFRWEDRDNPCLDAYYSPDRNVSRNILASNLGLIAK
                     MGEDNILHVSVNDLLTAMPVTDVRITAYDYQMQQIVSGSTDPEGSFSLYCPGKPFLVT
                     AEKDRDRNYLKVNDGSSLPLSSFDVSGTRPEKGIKAFIHGERDVWRPGDSIYLSLFIR
                     DMLNDLPADHPVQFELINPLEQRVDNQIHKPEGKRLIVVRTATGADAITGNYSAQFRI
                     GGAVFTKRIRIETIKPNRLKINLDFPSEILGGSERSTTGNLGVKWLNGAVAGNLNASV
                     DYLLKHTKTEFPKYSQYVFDDPVAEYYTETVNIFNNRIDDNGNALIAFSPGEQFSAPG
                     MLNALFTVRVMEPGGDESIIQSDYKYAPFPVFVGISLPELGDKGRTLYTDRDNQVRIV
                     TLNEKGIPVSSEVELNIYKLSYRWWWESDDEYLADYISGRIHKPVIRQKVIVPAGGTG
                     IRFNIGRKEWGRYLVRASTPSGHSTGKIVLVDWPWEYGMKPGADGATLLAVNTDKEKY
                     HPGDEVRLSFPSPENARAIISIENATGVLNQINTRTDRGSTEVRFRVTSGMAPNVYAH
                     VSVIQPHGQTVNDMPVRLYGIAGIMVEDKDSRLQPVINAPAEVRSRQSFAVSINEASG
                     RAMSYTLAVVDEGLLDITGFRTPDPWNYFYAREALGVRTWDLYDQVFGSFGGTLDRIF
                     AVGGDESLIDRSASKAQRFIPVVKFLGPFHLRQGKTNTHMITLPQYTGSVRAMVTAGN
                     ETAYGASDRSILVRDPLMVLATAPRVIGPGEKAALPVTVFINSSNIREVSLKAEGNGF
                     ISFPKASETLSAAGSTELNSEFAFIAGGKTGIAGIKVTATGGGETAVHEIQIPVRNPN
                     PPETRSELKLLKPGEKWETSFTPFGTEGSSSAVIGLSGLPSANLEKHLDYLLNYPHGC
                     TEQIVSAAFPQIWLGDLFKNDEATAAQSATNIKEAIGKILSRQMVNGGFGAWPGSYQA
                     DNWITSYTGHFMTEAGRKGYNITSGFLQKWTAYQKKAAREWRY"
     gene            complement(4670..5572)
                     /locus_tag="PLZ75_09600"
     CDS             complement(4670..5572)
                     /locus_tag="PLZ75_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72686.1"
                     /translation="MKVNIYLLLTFVFLITFSCRKKAPEKISFTPVFSTLSENSSADT
                     GPETKYETYYGLLTPVEICSIFNRLGVPYNNAALNPVSNSDLYMSMAKAAVNTGIYGV
                     DFGYLKMFGLGQEVINYMVTIRRISGKLGIPDYLMLNPLKQIESDIADPDTVTALMEK
                     AFSDLENHLMRNERESIAGLMLMGGWVEAMFIATQLAYDHENPDPVVIQKIAEQKYTL
                     KSLLSFMKNYYDDPVVVYYTKKLKFLNNYFDSFDIFFKPGDLEIDTLKKVLRSSGADM
                     TITLPTLDIIKDYVAKLRTEMVSP"
     gene            5864..5986
                     /locus_tag="PLZ75_09605"
                     /pseudo
     CDS             5864..5986
                     /locus_tag="PLZ75_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
BASE COUNT         1425 a         1468 c         1454 g         1717 t
ORIGIN      
        1 catacctcca ttcccgggcg gcctttttct gataagccgt ccatttctgc aagaatcccg
       61 atgttatgtt atatcccttc cttccggcct ctgtcatgaa atgcccggta taggaagtta
      121 tccagttgtc ggcctggtaa gagccgggcc aggctccgaa tcctccgttt accatctgcc
      181 ttgaaaggat tttaccgatg gcctctttta tatttgttgc tgactgtgcg gcagtggctt
      241 catcgttttt aaacaggtct ccgagccata tctgcggaaa tgctgcagaa acaatctgtt
      301 cagtacagcc gtgagggtaa ttgaggaggt agtccagatg tttttcaagg ttcgctgaag
      361 gaagtcctga cagtccgatc actgctgaag aggatccctc agttccgaaa ggagtgaagg
      421 atgtttccca tttctctccc ggtttcagca gtttaagctc tgatctggtt tccgggggat
      481 tgggattccg gacggggatc tgtatttcat gaacggctgt ttcgccacca ccggtggcag
      541 tcactttgat tccggcaatc cctgtcttac cgcccgcgat aaatgcaaat tcgctgttca
      601 gctcggtgct gccggctgct gacaaggttt ccgaagcctt cgggaagctt atgaatccgt
      661 tgccttcagc tttcaggctc acttccctga tattgctgct gtttataaaa acggtaacgg
      721 gaagcgcggc tttttctccc ggaccaatga ctctcggggc agtggccagc accatcagag
      781 gatccctgac aagtattgac ctgtctgatg ctccgtaggc tgtttcattc ccggccgtta
      841 ccattgccct gacagatccc gtgtactgcg gaagggtgat catgtgtgta ttcgtctttc
      901 cctggcgaag gtggaagggt ccgaggaatt tcactaccgg aataaatctc tgggccttgc
      961 ttgccgatct gtcgatgagg ctttcgtcgc ctccgaccgc aaatatcctg tcaagtgttc
     1021 ccccgaagga tccgaaaacc tggtcgtaca gatcccaggt ccttaccccg agtgcttccc
     1081 tggcgtagaa atagttccat ggatccggcg ttctgaagcc ggtgatatca agcaatccct
     1141 catccacaac agcaagggtg taagacattg ccctgcctga ggcttcattt atactgaccg
     1201 cgaaggactg ccgcgacctg acctcggcag gggcatttat cacgggctga agccgtgaat
     1261 ccttgtcttc caccattata ccggctatgc cgtaaagccg gacaggcata tcattgacag
     1321 tctggccatg aggctgaata accgatacat gtgcataaac gttcggagcc attccggaag
     1381 taaccctgaa cctgacctca gtgctgcccc tgtccgttct cgtgttaatc tggttcagta
     1441 ctcccgtggc attttctatt gatataatgg cgcgtgcatt ttccggtgaa gggaaggaca
     1501 atcttacctc atcgcccgga tgatatttct ccttgtcggt attaactgcc aggagggtgg
     1561 ctccgtcggc acccggcttc atgccgtatt cccatggcca gtcgacaaga actattttcc
     1621 ccgttgaatg tcctgatggt gtgctggccc gtaccagata cctgccccat tcctttctgc
     1681 ctatgttgaa cctgatgcct gtcccgccgg cagggacgat taccttctgt ctgatcacgg
     1741 gcttgtggat gcgcccggaa atataatccg ccaggtattc atcgtccgac tcccaccacc
     1801 acctgtatga cagcttgtaa atgttaagct ccacttccga gctgaccggt atgccctttt
     1861 cattcagcgt tactatcctt acctgattat ccctgtctgt atagagagtt ctgcccttat
     1921 cacccagttc cggcagactg attccgacaa aaacagggaa gggagcatat ttatagtctg
     1981 actgtatgat gctttcgtct cctcccggtt ccattaccct caccgtaaaa agtgcattca
     2041 gcattccggg tgcactgaac tgttcacccg gactgaatgc tatcagcgca ttgccattgt
     2101 catcgatcct gttattgaaa atgttaaccg tttcagtata atattccgca accggatcat
     2161 caaagacata ctggctgtat ttcggaaatt cggtttttgt gtgtttgagc agatagtcaa
     2221 cagaagcatt gaggtttcct gccactgcac cgttgagcca tttcacaccg aggtttccgg
     2281 tggtgctccg ttcgcttccg ccaagtattt ctgaagggaa gtcaagattt attttcagac
     2341 ggttgggttt gatggtctca attcgtatcc gttttgtaaa gacggcacct ccaatcctga
     2401 actgggcact gtaatttccg gtaatggcat cggcacccgt cgcggttctg acaacgatca
     2461 gcctttttcc ctccggttta tgtatctggt tgtccactct ctgttcaagg ggattgataa
     2521 gttcgaactg cacgggatgg tcagcaggca gatcattcag catatccctg atgaagaggg
     2581 aaagataaat cgagtcgccc ggtctccata catccctttc gccgtggata aatgccttga
     2641 ttcccttttc cggccttgta cccgatacat caaaggaact gagcgggaga gatgatccgt
     2701 cattcacctt caggtaatta cggtccctgt ccttttctgc ggttacaagg aagggctttc
     2761 caggacagta gagtgagaag gaaccttccg ggtcagtact tccggatact atctgctgca
     2821 tctggtagtc ataggccgtt atccggacat cggtgacagg cattgcggtc aggagatcgt
     2881 taacagacac gtgaaggatg ttgtcttcgc ccatcttggc tatcagtccg agatttgatg
     2941 ccagaatatt cctcgaaacg tttctgtcgg gactgtaata tgcgtccaga caagggttat
     3001 cacggtcctc ccagcggaat cccattgaat agtagatgga ttcatgactg tcgtcaaaat
     3061 agtcatcagg atcatcatag tatgaccttt ccatttcccg ggcctcatcg aggagttctt
     3121 caaagcgtga gggacgtccg tcgccggaac atgaaagcgt tgaataggat ctcctcatgc
     3181 tgagccttac attgtaaagg acacccggtt caacatttat ataatcggca aggtcgatgg
     3241 tgtagagatt ccatgatccc gttccggctg cggttatggc tccgctgttt tccgttcccg
     3301 tgcccggact gtttccggct gccgggtttc cggttcccgt gcttgccaat ccgcctgatc
     3361 cgctttccgg gttcaggtca actctcccgg agtaaaccgg ccggccgaag cgtttgatat
     3421 aatatcccct gtccatttca ctttcctgca ggaaataggg gaggttgttt tcaaatattt
     3481 taatgatttt caggtcaaca gccttcaggt tggctgccct gaacgggaat atcaggtttt
     3541 gtgacgatgg cacgataaga cctttgccaa taagccttat ttccggtctg agaggcgcaa
     3601 attcgagttt tgatgaaaag gatgaagaga gagttttgcc ttttatattt ctgagtgagg
     3661 gttcaatgtt gagtgtcact gtttggggaa gcctgtcggc gggaagaaga ctcagcacat
     3721 tcctgctgat gcttgtcgtg aaccgcatgg caggttccag gaagacaagt ccttccgttt
     3781 cctgcgattc atccaccgga tccgagaaga caatatcaat tgccggattg tcacctgaac
     3841 gggttctgac atccattaca atgaaatcac ccgaagccgg tatgctgata ttttgtgatc
     3901 cctttctcct gattcccgaa acggtgccgt cccatgagat tgtcagttcc cctgcccttt
     3961 cttccctcac tatgtcagtc agcgtgaacc tgtgaacgag attgtcctca tgctcccatt
     4021 ttattacggt tttccttctg ttcagccttg catcaacaaa gctttctgct tccgcatcag
     4081 gaataaaatc cgctgttgtc agttcccctg ttatggtata cctgtcgcct tccggactgc
     4141 tttcagggat accgcagctt atggtgaaat ctttttttag tgtttgtatt ctgattggga
     4201 attctttcag tctttcctca acgtttccga gcttgtggat attcagtctg ccggtatatg
     4261 ttttccccgg atcgagaggc tttgacggtc tgaataccag ggttgtttca tcggcccatt
     4321 cggttttgcc ctttactgcc ggactgaact cgaaaagtcc ggagggcagc cgtttgcccg
     4381 cagcggaggc aaattcctga gtgaagcgta tttctatggc agaagccgca ggtatgattc
     4441 ccgatgtata ggccgagata tagcggctga agccattgtc caggggcaga actgtccctg
     4501 taagtccggg aaccttcgga agttcctgtt tttgtgagga tttgtcaccc cgcccgcatg
     4561 aaacagcgat ggcgataaaa atcgtagcga tgaaaaaccg ggagaatttc atgatgaaaa
     4621 cagataaacg ggtttagtat ttaaaattac gaattccccg gataatattt caggggctta
     4681 ccatctcggt tctcagcttt gcgacataat cctttattat gtcaagggtt ggaagggtga
     4741 ttgtcatgtc agcacccgag gaacgcaata cctttttaag ggtatctatt tcaaggtcac
     4801 cgggtttgaa aaagatgtcg aagctgtcga agtaattatt gagaaatttc agttttttcg
     4861 tgtaataaac caccaccggg tcgtcataat agtttttcat gaaactcagc agtgatttca
     4921 gggtgtattt ctgttcggcg attttctgta ttaccaccgg atcgggattt tcgtgatcat
     4981 aagccagctg ggttgcaatg aacattgctt ccacccatcc gcccatgagc atcaggcctg
     5041 caatactttc cctttcgttt ctcatcaggt ggttctccag gtcagaaaag gctttttcca
     5101 tgagggcagt aacggtgtcg ggatctgcaa tatcgctttc aatctgtttg agaggattga
     5161 gcatcaggta gtccggaatt ccgagcttcc cgctaatcct gcgtatggta accatgtagt
     5221 tgatcacttc ctgtcccagt ccgaacattt tcaggtaacc gaaatccacc ccgtaaattc
     5281 cggtattcac ggcagctttc gccatgctca tgtataagtc gctgtttgat accggattca
     5341 gggctgcatt attataaggc accccgagcc tgttgaagat ggagcagatc tcaaccggag
     5401 taagcagtcc gtaataggtt tcatattttg tttccgggcc ggtatctgcg gaggaatttt
     5461 cagaaagggt tgaaaaaaca ggagtgaagc tgattttttc cggagccttt tttctgcagg
     5521 aaaaggtaat gagaaagaca aatgtcagta ggagatatat attgactttc atggacagag
     5581 aattcacaga ccaaatttat aataaaattc cgaaataatg cgcaaccggg gcagattcat
     5641 tgatgcgttt ttccggccgg gtttcaggat taaaagctgc acctgatata aattatagcc
     5701 aatgtcagca caaaaatgaa agaatattga tatccaggca aattattatt tacctttgtg
     5761 ctgtagtatc ctctcagcag gcaatgggct gttctgaact ttataagatt ctgacaaaag
     5821 atggctggtc tctccttttt cacaataagg ttcacatgta aaacttaaac atgacaagaa
     5881 accatgtatg atcatcttcc caaatcatgg cagtcaggaa gcagaaaaag gacttgagga
     5941 aaagatactc gaggatgctg gtcagaagtt ggagaataaa aagtgatgga gatgaaaaca
     6001 acaaacagaa aaaattctgc ttggaattca tcaaatcgga caggaactat cagagataaa
     6061 tctt
//
LOCUS       DAOSPX010000196         6023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12025_length_6023_cov_3.801273, whole genome shotgun sequence.
ACCESSION   DAOSPX010000196 DAOSPX010000000
VERSION     DAOSPX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12025_length_6023_cov_3.801273"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLZ75_09610"
     CDS             complement(<1..347)
                     /locus_tag="PLZ75_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS72687.1"
                     /translation="MKQKNYLWLVILLTGLFFSACKQADNKLTEEEIRDGWILLFDGK
                     TLDGWRDFKGEPGTVTAPWKVENGTLTALGQGSDSTGYIVSEKEYGNFIISFDWKIEK
                     AGNSGLLYHVVERP"
     gene            559..1116
                     /locus_tag="PLZ75_09615"
     CDS             559..1116
                     /locus_tag="PLZ75_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOS72688.1"
                     /translation="MALFIIALLITVGGFSLYVYNRLVRLSNYVEEAWSGIDVQLRKR
                     HDLIPLLVSTVQGYAGHEKKLLEAITSLRVQGMSASGVRESEKAEVELSKALGKLFVV
                     IENYPELKASQNFLELQRDISRVENDLQKARRYYNGTVRNYNILVGQFPSSLVAKATD
                     HHPREFFDIGDESERAVPGFKILNE"
     gene            1157..2899
                     /locus_tag="PLZ75_09620"
     CDS             1157..2899
                     /locus_tag="PLZ75_09620"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOS72689.1"
                     /translation="MCAFPLTRSTGIIAKIRATCLFLFSAFLAAGFTAAAQERILSYD
                     ILAEIEKDGTLLVSEQISVVAEGNAIKRGIYRSFPTKYTDRLGNRFRTGFEVVKVLRN
                     GSPEPWFVKTQSNGVVVYIGDTDTELIPGTYDYTLVFKTTRQIGFFDAFDELYYNAIG
                     GDWSFAIEKASVKVRLPEGAEILQKAAWSGYAGSTGCDCELVESGNTLIVSTTRALQP
                     REQLTLAVAWPVGFVHHPSASEKTLYFLRDNFHVLFALAGLLMVLLIYYRKWKEVGKD
                     PARGTIIPLFDPPGSFSPGETAYLTGMGLKDRVMSSALVNMAVKGYLIISQSDRGYSV
                     GQLGRQTEELSAEEKAIASALFSRGPEIELDDKNYRLFGEARKNADKVLREKMIPEYF
                     SLNTRQLLSGFLLSVVWTVLTFMFSPSPLVPVILIILLVALAILFTWLIKAPTVQGRA
                     LMDEAEGFKMYLSVAEKDRLNMMGEPGLTAERFEKYLPYAIALGVENEWGRRFENALK
                     RSEQDAGDYRPSWYTGPAAGISGFSPQAFSSSVGKSFSSAISSASVPPGSSSGSGGGG
                     SAGGGGGGGGGGGW"
     gene            complement(2950..>6023)
                     /locus_tag="PLZ75_09625"
     CDS             complement(2950..>6023)
                     /locus_tag="PLZ75_09625"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS72690.1"
                     /translation="YRSEEGSPLKGGIDLTPLIRNGRLTWEAPEGTWEIWTYRTVRKE
                     GSLNPLMPGAGDTVLNGFFRPFEKMSSGKASKGLNYFFNDELQLAVGKYAWSPDFPEE
                     FRKRKGYDLFEVLPAMWVDIGNISPKVKMDYADVRMSLIEERYFRPIYLWHAERGMIF
                     GCDSWGRGLDPSEFGDYFRATRWYTAPGHDTPGGKADLIKGRVSSSIANLYRRPRVWL
                     EGYHSLGWGASPEQLMYATRENFLYGCTLLNLHGLYYSTYGSFWEWAPPCYHFRMPYW
                     DHMDSFLSYFDRLSWLMSQGHTVCDVAIVYPIAPYEAEMDGETARNTSFELGEKLMAA
                     GISFEFIDNESLARAVVENACLNVKHAGASYKALVFADMNAVRWQSIEKAAAFAGNGG
                     NVFSVGALPSASDRTGRSDGELIRINDLTFKDNCRFNTVDEAVEAIRNSFKQDVTGIG
                     QTVRYLHRKAGFRDVYMVMDAKPGSTVEFRCKGRVELWDPWTGETKALRVKGETPEGT
                     LVELPLEEYEAQIVVFTPGKKHVNPPETPESGVSGEIYFPLDWNVSFIPTMDNKWGDF
                     RLPVTGRRMIGPEARRFRWARETKDMAETAMLPETDDSTWEEKLHGYGSQLYVLGPVS
                     KNIGVYYFERKLAEMKHLDPSVPEYVYGRKFEWEAYDFSWRRGKEGDPGHQGYHGLKR
                     TVSNDFLCLGKPEQALNEIRYVDEIPEGRYYIWTTVTVDNDLTADILFSDQPPQDKSH
                     TSPVLTPSVIYVNGTLVSFPDNGVNLKAGVNTILARYDHAGRGHFVLRLHGAPEIPAR
                     RPLSMKWADDDAVIPFDIYAGKSAAEWFRFLSAPGTSAISFETPGEAEVWIDGMPMKK
                     QAEGRFVAEKVPEKAAVVAIRIIPPRPGITGGALIPEPVEVETDGSGLMRTGDWSECG
                     ILKNYSGGIRYRNKVSITSAQVKSGIIADLGKVAGTAAVYVNGKKAGVRVAPPWKTDI
                     SAFVKKGENTIEVIVYNTLANHYQTIPSLYKGDPVSGLLGPVRLLVEERKK"
BASE COUNT         1454 a         1484 c         1454 g         1631 t
ORIGIN      
        1 ggacgctcaa ccacatggta aagaagaccg ctgttgccgg ctttttcaat tttccagtca
       61 aacgatatga tgaaattgcc atattccttt tcggaaacga tgtatccggt actgtcgctt
      121 ccctggccca gagctgtaag ggttccgttt tcaaccttcc atggtgcggt aactgttccg
      181 ggttcaccct tgaaatcacg ccatccgtca agtgtttttc catcgaaaag aagaatccat
      241 ccgtcccgga tctcttcctc agtcaactta ttgtctgcct gtttgcaggc tgagaaaaat
      301 aatcctgtca acagaatcac aagccataaa taattttttt gtttcatatt gtttaaatat
      361 taagatggta ttagaatgat ttcagagaat aagctgcccg gttaacgggt tgaaaggata
      421 aaaatagtaa aaaagtcctg aatgggctaa aagctttaaa aaccttttag aaacattttg
      481 accggacaac agttattcct gctttttttt atatttgaac aggcagatgc cggtaatatc
      541 tgccggaaat tcatgacgat ggctttattt atcattgcat tattgattac agtcggggga
      601 ttctccctat atgtctacaa caggctggta aggctttcca attacgtaga ggaagcctgg
      661 agcggcattg atgtacagct caggaaacgt catgatctga ttcctcttct tgtcagtaca
      721 gtacagggat atgccggtca tgagaaaaaa ctgctggagg cgataacttc actgcgggta
      781 caggggatgt cggcttcggg tgtcagggaa tcggaaaagg ctgaagtgga actttcaaaa
      841 gccctcggga aactttttgt ggtgatcgaa aactatcccg aactcaaggc cagtcagaat
      901 ttcctggagc ttcagagaga catttccagg gttgaaaatg atcttcagaa agccaggcgt
      961 tattacaacg ggacagtccg aaactacaac atccttgtcg gacagtttcc ctcatccctt
     1021 gtggcaaagg ccacggatca ccacccccgg gaattttttg atataggaga tgaatcggaa
     1081 agggctgtcc ccggatttaa aattttgaat gaatagttac atacggcagg atgccggtta
     1141 aaaaaagtaa aaagaaatgt gtgctttccc tcttacccgc agtacgggca ttattgcaaa
     1201 gatcagagca acatgccttt ttctgttctc tgcctttctt gctgccggct ttacggcagc
     1261 tgctcaggaa aggatactgt catatgatat tcttgcagag atagaaaaag acggcacttt
     1321 gctggttagt gagcagataa gtgttgtggc cgaaggcaat gcaataaaaa gagggatata
     1381 ccggagtttt cccacaaagt atactgacag gctgggcaat cgtttcagga cgggtttcga
     1441 agttgtgaaa gtattaagga acggctcgcc ggaaccctgg tttgttaaaa cgcagtcaaa
     1501 cggtgttgtt gtttacatag gcgatacgga tactgagctt attccgggta catatgatta
     1561 tacacttgtc ttcaaaacta cccggcagat aggttttttt gacgcttttg atgaattgta
     1621 ttacaacgcg atcgggggtg actggagttt tgcaattgaa aaggcttcgg tgaaggtaag
     1681 gcttcctgaa ggagcggaaa ttcttcagaa ggcagcatgg tcgggatatg ccggttcaac
     1741 cggatgcgac tgtgaactgg ttgaaagcgg caataccctg attgtatcca ccacacgtgc
     1801 attacagccc cgggaacagc ttacccttgc tgttgcctgg cctgtgggtt ttgtccacca
     1861 cccttcagcc tcggaaaaaa ctctttattt tctcagggac aactttcatg ttcttttcgc
     1921 gctggcaggg ctgttgatgg tcctgctgat ctattacaga aaatggaagg aggtcgggaa
     1981 agatcctgcc agggggacta tcattcccct gtttgatcct ccgggaagtt tcagtcccgg
     2041 agaaaccgct tatcttacag gaatgggact gaaagacagg gtaatgtcct ccgcgctggt
     2101 aaatatggcc gtgaagggat atcttataat cagtcaatcg gacaggggat acagcgtcgg
     2161 gcaactgggc agacagacag aagaactcag tgctgaggaa aaagctattg catcggcact
     2221 tttttcccgc ggaccggaga tagaacttga cgacaaaaac taccggctgt ttggtgaagc
     2281 caggaagaat gctgataaag tactgagaga aaaaatgata ccggaatatt tcagtcttaa
     2341 cacccggcag ttgctgtccg gttttttact atcggttgtc tggactgtac tgacttttat
     2401 gttttctcct tcccccctgg tccctgtgat tctcattatt ctgctggtgg cacttgccat
     2461 cctgttcaca tggcttatca aggctccgac tgtgcagggc agggcattga tggacgaggc
     2521 cgagggtttt aaaatgtatc tttcagtggc tgaaaaagac cggctcaaca tgatgggcga
     2581 acccggactg acagccgaac gctttgaaaa atatcttccc tatgccattg ccctgggtgt
     2641 ggagaatgag tgggggagaa ggtttgagaa tgcccttaaa cgatctgaac aggatgccgg
     2701 tgattacagg ccttcctggt atacaggtcc ggcagccggt atctccgggt tttccccgca
     2761 ggccttcagc tcgagtgtgg gaaagagttt ttcctctgca atatcttccg catcggtgcc
     2821 tccgggttca tcttccggca gcggcggcgg aggaagtgcc ggcggtggtg gtggcggcgg
     2881 cggtggcggc ggctggtagg cctaaacgtt ccgcagggct tgtatgctct gacaattatt
     2941 tttccctgat tattttttcc gttcttccac aagcagtctg acagggccga ggagtcctga
     3001 cacgggatcc cccttgtaga gcgaagggat tgtctggtaa tggtttgcaa gggtattgta
     3061 aacaatcacc tcaattgtat tctcaccttt tttcacaaag gctgatatat ctgtcttcca
     3121 cggtggagca accctgacac ctgctttttt gccgtttaca tatacagcag ctgtcccggc
     3181 aacctttcca agatcggcaa taatgcctga tttaacctgg gctgatgtaa tgcttacctt
     3241 gttcctgtac cggattcctc cggaataatt tttcaggata ccgcactccg accagtctcc
     3301 tgtccgcatc aggccgcttc catccgtttc tacctcaacg ggttccggta tgagtgcccc
     3361 tccggtgatt ccgggacggg gcggaataat ccttatggca acaaccgcag ctttttcagg
     3421 aaccttttcc gcaacaaatc ttccttcagc ctgcttcttc atgggcatac cgtcaatcca
     3481 gacctcggct tcccccggtg tttcgaaact aatagccgat gtgccggggg cagacaggaa
     3541 cctgaaccat tccgctgctg attttcctgc atagatgtcg aagggaataa cagcatcatc
     3601 atctgcccat ttcattgaca gcggtctgcg ggcagggatt tccggagccc cgtgcagtct
     3661 cagtacgaaa tgtccgcgtc cggcatggtc atatcttgcc aggatagtat tgacgccggc
     3721 tttaaggttt acaccgttat cgggaaagga tactagcgtg ccgttcacgt agattactga
     3781 aggagtcagc accggagaag tatggctttt atcctgcgga ggctgatcgc tgaaaagaat
     3841 atcggctgtc agatcattgt ccacagttac cgtggtccag atataatagc gtccttcagg
     3901 tatttcatcc acgtagcgta tctcattcag agcctgttcg ggttttccca ggcaaaggaa
     3961 atcattgctg acagtccgct tgagtccgtg atatccctgg tgacccggat cgccttcctt
     4021 tccccggcgc caggaaaaat catatgcttc ccattcaaac ttccttccat atacatattc
     4081 cgggacggat gggtcaagat gtttcatctc tgccagtttt ctctcaaagt aataaacacc
     4141 aatatttttc gacacgggtc cgaggacata cagctggctt ccgtaaccgt gaagcttctc
     4201 ttcccaggta ctgtcatcag tttccggaag cattgccgtt tctgccatat cctttgtttc
     4261 ccgcgcccat ctgaaccggc gggcttccgg ccctatcatc cttctgcctg ttacaggcag
     4321 tctgaaatca ccccatttat tatccattgt cgggataaaa ctgacattcc agtcgagggg
     4381 aaaatatatt tccccggaaa caccggattc cggtgtttcc ggggggttga catgcttctt
     4441 gccgggtgtg aatacaacaa tctgggcttc atattcctca agaggaagtt ccaccagagt
     4501 accttccggg gtttctcctt tcaccctcag ggctttggtc tctccggtcc agggatccca
     4561 tagttcaacc cttcctttgc agcggaattc aactgtcgat cccggtttcg catccatcac
     4621 catgtaaaca tcccggaacc cggctttacg atgcaggtag cggaccgtct gtccaatacc
     4681 cgtcacatcc tgtttaaagg aattccggat tgcctcaact gcctcatcaa ctgtgttgaa
     4741 gcgacaattg tccttaaaag tcagatcatt gatacggatg agctcgccat cgcttcttcc
     4801 tgttctgtcg gaggcagacg gcagtgcgcc gacagaaaaa acatttccgc catttccggc
     4861 aaatgctgcc gccttttcaa tgctctgcca tctgactgca ttcatatccg cgaaaaccag
     4921 tgccttgtag gatgcccccg catgtttcac gttcaggcag gcattttcaa caactgcccg
     4981 ggcaagcgac tcattgtcga taaattcaaa acttattcct gctgccataa gtttttctcc
     5041 cagctcaaaa gatgtgtttc ttgcagtctc gccgtccatc tcagcctcat agggtgcaat
     5101 cggataaaca atggcaacat cacataccgt gtgtccctgg ctcatcagcc aggaaaggcg
     5161 gtcgaaatat gacaggaagg aatccatatg atcccagtag ggcattctga agtgatagca
     5221 tggcggtgcc cattcccaga aggatccgta ggttgaatag tacaggccgt ggagattcag
     5281 gagggtacat ccgtagagga aattttcgcg tgttgcatac atcagctgtt cgggacttgc
     5341 tccccagcca aggctgtggt agccttcaag ccataccctc ggcctcctgt aaaggttggc
     5401 aatggaagag gatactcttc ccttgatgag gtcggccttg ccgccgggag tatcatggcc
     5461 cggggcggta taccatcgtg ttgcacggaa gtagtcacca aactctgacg gatcgagtcc
     5521 gcggccccac gaatcacatc cgaatatcat tcctctttct gcatgccaca ggtatatcgg
     5581 tctgaaatag cgttcctcga taagcgacat ccggacatca gcataatcca ttttgacctt
     5641 tgggcttata ttcccgatat cgacccacat ggccggcagt acttcaaaaa ggtcatagcc
     5701 tttccttttc ctgaattctt ccggaaaatc aggactccat gcatatttgc caaccgcgag
     5761 ctgcaattcg tcgttgaaga aataattcag tccctttgag gcttttcctg aactcatttt
     5821 ttcaaacggc cggaagaaac cattcagtac agtatctccc gcccccggca tcaggggatt
     5881 tagtgaaccc tcttttctta ctgtcctgta tgtccatatt tcccaggtgc cttcgggtgc
     5941 ttcccatgtc agtctgccat ttctgataag gggtgtaaga tcaattcccc ccttgagggg
     6001 agatccttcc tctgacctgt ata
//
LOCUS       DAOSPX010000197         6022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12027_length_6022_cov_4.209988, whole genome shotgun sequence.
ACCESSION   DAOSPX010000197 DAOSPX010000000
VERSION     DAOSPX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12027_length_6022_cov_4.209988"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLZ75_09630"
     CDS             <1..536
                     /locus_tag="PLZ75_09630"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOS72691.1"
                     /translation="MFNSLNVLSGLIERDVTKAQQFIDEFSQIYRYVLETIEQPVTTL
                     GRELEFVRSYLFLQQMRYGEDLSWSVNLPAGVLELALPPLSVQAVLENAIKHNIVNHS
                     KPLHIDIGGEEMMLLVSNRLQPKMSTSGSTGVGLQNLTKRYSLISDRLPQFTIENDHY
                     IARLPLINPETDEDTDN"
     gene            517..1272
                     /locus_tag="PLZ75_09635"
     CDS             517..1272
                     /locus_tag="PLZ75_09635"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOS72692.1"
                     /translation="MKILIIEDESVAADKLEKMLLEIDPSITVAGKRGSIRDSVKWLM
                     QNRADIIFLDIQLSDGISFSIFEQVEVNTPVIFTTAYDQYAVRAFQLNSIAYLLKPIR
                     RSELAESLRKYQSLRAAFSIDFDALLTQLQGRDPGYRKRFLVQTGEKIRKIEVQDIAW
                     FNALEKSVYMKTSQGQSFPSEFSLDGLERILDPACFFRINRKYLVNINAIAGMTAWSR
                     GRIKLELRPKADNELDAVVSIDRSAAFRRWLNA"
     gene            complement(1326..2267)
                     /locus_tag="PLZ75_09640"
     CDS             complement(1326..2267)
                     /locus_tag="PLZ75_09640"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72693.1"
                     /translation="MKKRIAACYRQVTMNAFVLSVLVFSFVRPGPVSAQVHEFTREQM
                     EIFTALNPYGRFPDGRPMIPDAVIDSVRNLKIQTIEALEALAMHKYNNSQFDGGWQSL
                     IPGEKIVGRAFTVQFMPPRPDLQEGIRNMAKKNNTMPVSKSATIDMLQKNDLAVIDLY
                     GKVEGGLPYVGDKLAYYIWKTTGTGFIVDGGIYFLETMAQSGIQAFYRGGSPLTNTGS
                     AIVTGVNIPVRIGDAVVMPGDLVIGDEDGIVAVPPHLVSKVIKTTIMNRRRDVWIKKA
                     FDLGRYKSSEIYGRMQEPLQTQFNKYRETGDPQYLPK"
     gene            complement(2348..3658)
                     /locus_tag="PLZ75_09645"
     CDS             complement(2348..3658)
                     /locus_tag="PLZ75_09645"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HOS72694.1"
                     /translation="MKKQIIFLLLLVLIAGCRNEAPEASKVPVEKIYKYVIKGGHLID
                     PKNNIDEVMDIAISAPTNPDRGYDRGSRDGKIALVAKNINPDLGEQVIDARGLYVSPG
                     LIDMHVHVFWGSDMEGTYRNGPMSVPPDGFALRTGVTTVVDAGCAGWRNFEVFKEQTI
                     DFSNTRVLAFLNIVGEGMAGGKYENDLNDMDARKTADMILKYPEHIVGIKNAHYYGPG
                     DTYLIPIRRGIEAGELAGGKPLMLDGRLGEEIFDLFRPGDIFTHVYGRAIVDSADNIK
                     PFVLKAREKGIIFDVGFGGASFRLPIAEPALNAGFFPDAISTDLHINSMNSAMKDMPN
                     IMSIFLAKGMRLAEVIEASTWNPAKYIQREDLGHLSVGAVADVTIFDIRKGDFGFLVI
                     GGEIRGTQRIENEITIRGGEIVYNLNGRISPVTLPPAPGQRGRI"
     gene            4143..5285
                     /locus_tag="PLZ75_09650"
     CDS             4143..5285
                     /locus_tag="PLZ75_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOS72695.1"
                     /translation="MKNIIRLISVFLLLHISQGHSVAQYDDASVFPVKGLSIEVPRPK
                     DVDKFVKFIAEELAPRGVNTLFLLESLNYKFKSYPQLADSLALTTREVKKIVSACRKH
                     NIRIIPQINLLGHQSWGTNVGILLKTFPEFDETPHIKITEANQGSPAEGGLYCKSYCP
                     LHPDIHKVLFAVIDEICDVYEADAFHGGMDEVFYIGDDRCPRCRGYDKAVLFADEVRR
                     IYDHLKSRNRELWIWGDRLIDGRTTGYGIWEASFNSTWRAVDLIPKDVVICDWHYERP
                     DQSPVYFAMKGFRVVTCPWRTPPTALVQTEDMARWRKYATPEMKPRYYGMVATTWMDN
                     GSFIDGFYGNLTETAKKAVHPRNPDYTPWDTFRAMFRKMNELEEAK"
     gene            5515..>6022
                     /locus_tag="PLZ75_09655"
     CDS             5515..>6022
                     /locus_tag="PLZ75_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72696.1"
                     /translation="MKAFANHIRYTGICRKWTAVLVLLAGLISGPDAGAQYFGRNKPG
                     YRSFRFDVVQTPDFEIYHYLKNDTLVNMLTKWSEQWYKTHLYIFRDSFRTRNPIIFYS
                     NHPDFQQTNTISGLIGTGTGGVTESLKNRVIMPIAPSLAQTDHTLGHELVHAFQYNMF
                     IREDSSRRM"
BASE COUNT         1571 a         1414 c         1405 g         1632 t
ORIGIN      
        1 tcatgttcaa cagccttaat gtactgtcag ggctgataga gagggatgtc acaaaagcac
       61 agcagttcat tgacgagttc tcgcagatat accgttatgt actcgagaca attgaacaac
      121 ctgttacaac acttggcagg gagcttgaat ttgtgcgctc ataccttttc ctgcaacaga
      181 tgcgatacgg agaagatcta tcatggtcag ttaacctgcc tgccggggtt ctggaactgg
      241 ccctgcctcc attatctgtg caggcagtgc tcgaaaatgc catcaagcac aatattgtca
      301 accactctaa accattgcat atagacatag gaggagaaga gatgatgctg cttgtgtcaa
      361 accggctcca gccgaaaatg tccacatccg gttcaaccgg agtgggactt caaaatctca
      421 caaagcgata ctcacttata agtgaccggt taccgcagtt taccattgag aacgatcatt
      481 atattgcaag gctgccgctg attaatcctg aaaccgatga agatactgat aattgaagac
      541 gagagcgttg ctgccgataa gctggagaaa atgctgctgg agattgatcc ttccattact
      601 gtcgcaggaa agcgggggtc aatcagggat tcagtgaaat ggctgatgca gaacagggct
      661 gacatcatct tcctcgatat acagctctct gacggcatca gcttcagtat cttcgagcag
      721 gtggaagtca atacaccggt aatattcaca actgcatatg accagtatgc tgtaagggca
      781 ttccagctta acagtattgc ctatcttctc aaacccatcc gccgcagtga gctggctgag
      841 agcctgagga aataccagtc gctcagggca gccttcagta ttgactttga tgccctcctc
      901 acgcagttgc agggcaggga tccaggttac aggaagcgct tccttgtgca gacaggagag
      961 aagatcagga agatagaggt gcaggatata gcgtggttca atgccctcga aaaaagtgtt
     1021 tatatgaaaa cttcacaggg acagtcattc ccctctgaat tcagccttga cgggttggag
     1081 agaatccttg atccggcctg tttcttccgt attaacagga aatacctggt gaacataaat
     1141 gccattgcag gtatgacggc atggtcacgt ggacggatta agctggaact caggccgaaa
     1201 gctgacaatg agcttgatgc cgtggtgagc attgacagat ctgcagcttt cagaagatgg
     1261 ctcaacgcct gaattccaca gctaaatcac gttaagttat ctgtacagct gacgtttata
     1321 cctgattatt taggcaggta ttgcggatct ccggtttccc tgtatttgtt aaactgggtc
     1381 tgcagcggtt cctgcatgcg gccgtaaatc tcgctggact tatatcttcc gagatcaaag
     1441 gctttcttga tccatacatc gcggcggcgg ttcatgattg tcgtttttat caccttcgag
     1501 acaagatgag gcgggacggc cactattccg tcttcatcgc ctattacaag atcgcccggc
     1561 ataacaactg cgtcacctat acggacagga atattgactc cggtaacaat ggcggatccg
     1621 gtatttgtaa gcggtgatcc cccgcggtag aaagcctgaa tcccgctttg agccattgtt
     1681 tcaaggaaat agattcctcc gtcaacaatg aatcccgtac cggttgtctt ccatatgtaa
     1741 tatgccagtt tgtctcccac atacggcagg cctccttcca ctttcccgta caggtcgata
     1801 acggcaaggt cattcttctg aagcatgtca atggttgcag acttgctgac aggcattgta
     1861 ttgttctttt tggccatgtt ccgtatgcct tcctgaagat cgggccgggg aggcatgaat
     1921 tgaacagtaa atgcccttcc gacaattttt tcgccgggaa taaggctttg ccatccgcca
     1981 tcgaactgac tgttgttgta cttgtgcata gccagtgcct ccagtgcctc aatggtctgg
     2041 atcttcagat tccttacgga atcgatcacc gcatccggaa tcatcggtcg tccgtcagga
     2101 aaacggccat agggattcaa tgcggtaaat atttccatct gttccctggt gaattcatgt
     2161 acctgggctg ataccggtcc gggccggaca aacgaaaaca caagaacaga taaaacaaag
     2221 gcattcatgg tcacctgcct gtaacaggca gcaattcttt ttttcatttc aggacgcagc
     2281 tctgttttca tttctcaagg atttaactgg ttttcaggaa ttgtcaatta attgctgaaa
     2341 aggattatca tatcctgcct ctctggcccg gtgccggagg aagtgttacc ggactgattc
     2401 ttccgttcag gttatagacg atttccccgc ccctgatggt gatttcattt tcaatccgct
     2461 gtgttcccct gatttctcct ccgatgacaa ggaatccgaa atctcctttt ctgatgtcaa
     2521 atattgtcac atcggcaaca gctccaaccg acaggtgtcc cagatcttcc ctctgtatat
     2581 atttagcggg attccaggta ctggcttcaa taacttcggc cagtctcatt cctttggcaa
     2641 ggaatattga catgatattc ggcatgtcct tcattgcgga gttcatgctg ttgatatgca
     2701 ggtcggtgct gatggcgtca ggaaagaatc ccgcattcag tgccggttct gctatgggaa
     2761 gacggaaact ggctccgccg aaaccaacat caaaaataat tcccttttcc cttgccttca
     2821 gtacaaatgg ctttatgtta tcagcggaat cgacgattgc cctgccatag acatgggtga
     2881 aaatatctcc cggcctgaac aggtcgaaaa tttcctctcc cagcctgccg tcgagcatca
     2941 ggggtttgcc gcccgccagt tcacccgctt caatacccct tctgattggt atcaggtaag
     3001 tgtcaccagg gccgtagtaa tgggcattct tgattccgac aatatgttcg ggatatttca
     3061 gaatcatgtc tgcagttttc ctggcatcca tgtcattgag atcattctca tattttccgc
     3121 ctgccattcc ttcccccact atattcagga aagccaggac cctggtattc gagaaatcaa
     3181 ttgtctgttc cttgaagacc tcaaaattcc gccatcccgc acagcctgca tccaccacgg
     3241 tggtgactcc tgttctcagt gcaaagccat ccggcggaac actcatgggg ccgttcctgt
     3301 atgtgccttc catgtcgctg ccccagaaaa catgtacatg catatctatc agacccggac
     3361 ttacatacag gcccctggca tctataacct gttcccccag atccggattg atatttttgg
     3421 caacgagtgc gatcttaccg tccctgcttc ccctgtcata acccctgtca gggtttgtgg
     3481 gagccgagat ggcaatatcc atcacttcgt caatattatt tttcggatct atcaggtgac
     3541 cgccctttat tacatatttg taaatctttt caaccggaac ctttgaagct tccggggcct
     3601 cattcctgca gcccgcaatc agaaccagca gcagcaaaaa aataatctgt tttttcatca
     3661 taatttaatt ttgtttttaa tggtatgaca gccagtcccg cgaagcgttt tttgtttgtg
     3721 agcccggtca gtcacatgaa tttgaaacat gatttgttaa aagctatcgt gtttttacca
     3781 gtttccggaa tcgttgccgc catcccggga gatatctgca cagcaaatgc cggaagaatg
     3841 cagggaacga tagctgtgct aaaagtatgc ttttttttcc cttctgcaaa gattctccgg
     3901 ccaaatctgt ctcaggtggt gaataattca gccttacagc tatctgtggg gaggttttcc
     3961 gtttgcggca gccggaaaat gatccggccg gggatgaaaa agaaaggacg gcgggggaat
     4021 caaatgaaat aaaagctaat tttataattc catgcctttg aaaagaaggg gtaagatatt
     4081 gtaatctttc cggacatcgg ggaacagaac ccggtattcc attaaccaaa ataacgataa
     4141 tcatgaaaaa cataatccgt ttaatttcag tctttcttct tctgcatatc agtcagggtc
     4201 attcagtggc ccagtatgat gatgcatcgg tttttccggt caagggttta agcattgaag
     4261 tgcccaggcc aaaagatgtt gacaaatttg tgaaattcat tgctgaggag ctggctccgc
     4321 gcggggtcaa tactcttttt ctgcttgaat ccctgaatta caagtttaaa agctatcctc
     4381 agctcgctga ttcacttgca ctcacaacga gggaggtaaa aaagattgtt tccgcctgca
     4441 ggaaacataa tatcaggata ataccacaga tcaacctgct ggggcatcag tcgtggggca
     4501 ctaatgtcgg aatcctctta aagacctttc ctgaatttga cgaaacaccc catataaaaa
     4561 tcacggaggc aaatcaggga tctcctgcag agggcggact ctattgcaaa agctactgtc
     4621 cgctgcatcc ggatattcac aaggtcctct ttgccgtgat tgatgaaatt tgtgatgtat
     4681 atgaggctga tgccttccat ggcggcatgg atgaggtttt ctatattgga gatgaccggt
     4741 gtccgcgttg caggggatat gacaaggctg tactgtttgc cgatgaagta cgcaggatat
     4801 atgatcatct gaaaagcagg aacagggaat tatggatatg gggcgacagg cttattgacg
     4861 ggagaacaac aggatacggc atctgggagg catccttcaa ttccacatgg cgggccgttg
     4921 atcttattcc taaggatgta gtgatttgtg actggcacta tgaaagacca gatcagtcac
     4981 cggtttattt cgcaatgaag ggtttcaggg ttgtaacctg tccgtggagg acacccccta
     5041 ccgctcttgt tcagacggag gatatggcca ggtggcggaa atatgccacg cctgaaatga
     5101 agccgcgcta ttatgggatg gttgccacca cctggatgga caacggatca tttattgacg
     5161 gattttacgg aaacctgaca gaaactgcca aaaaagcagt tcatccccgt aatcctgatt
     5221 atacaccctg ggataccttc cgggccatgt tccggaaaat gaatgaactg gaagaagcaa
     5281 aataacattg ccggtttacc tgcaggtatt actgaaggac ccgtgttgcc tgataccggg
     5341 gagattcctg tgaaggatcc ggactgaagc cctgtgccgg ctgacatttt catggaggat
     5401 tcctgacact gaaacatttg tgctggcata ttttttgatc ttattttcca ggaaataatt
     5461 gaacatatga tattttttcc tgttaaatga tagattaaac cggaaacaaa tccaatgaaa
     5521 gcctttgcaa atcatatcag atacaccgga atctgcagga aatggacggc agttctggtt
     5581 ttgcttgcag gcctgatttc cggacctgat gccggagcgc agtattttgg cagaaacaag
     5641 cccggttaca gatcattcag gttcgatgtt gtacagacac cggattttga aatatatcat
     5701 tacctgaaga atgacactct ggtcaatatg ctcacaaaat ggtctgagca atggtataaa
     5761 acacatctct acattttcag ggattcattc aggacccgta atccgataat cttttatagt
     5821 aatcatcctg attttcagca gacgaacacg ataagcggat tgataggaac cggtaccgga
     5881 ggtgtcacgg aatcactgaa gaacagggtg ataatgccca tagcaccttc ccttgcgcag
     5941 actgatcata cactcggcca tgagctggtt catgcttttc agtataatat gttcatcagg
     6001 gaagattctt cgaggagaat gt
//
LOCUS       DAOSPX010000198         6017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12042_length_6017_cov_4.307112, whole genome shotgun sequence.
ACCESSION   DAOSPX010000198 DAOSPX010000000
VERSION     DAOSPX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12042_length_6017_cov_4.307112"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLZ75_09660"
     CDS             <1..274
                     /locus_tag="PLZ75_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72697.1"
                     /translation="NSLTADWYAAMQMYRDAEQRMDVYDEQYVLASKTLEIMLKGFSA
                     SSVLLTDVLRVIQQTLDYELMQVGALTDLNTAAAWLKRLMAVRENR"
     gene            286..2106
                     /locus_tag="PLZ75_09665"
     CDS             286..2106
                     /locus_tag="PLZ75_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS72698.1"
                     /translation="MKRKIYYRIFKGRWPVYILLLLLGVLLGRVFFHSGGHEDSAGHQ
                     HAGEEKVEIWTCSMHPQIRMTEPGKCPICGMDLIPLVSGGTVHHDHDAVHLSREAAEL
                     ANVHTTRVAAGDPAGEIRLYGMVQLDERLLQSQVSHVSGRIEKLFVNFTGETVTAGQK
                     LAEIYSPELVTAQQELLETAGTRQIQPLLYEASREKLRHLKLTDDQISAIENSGTIIS
                     SFTVVSNTSGTVISRRVSTGDYVSRGTVLYDIAGLSRVWLMLDAYESDLPFIRTGQQV
                     SFRVEAVPGRDFSGTVAFVDPLIDPLTRVAGVRVDVGNRSGMLKPGMFATGSLVSDMA
                     AYRNSIVVPKSAVLWTGKRSIVYVKDPQADEPVFRLREVKLGPAIGNAWVITGGLAEG
                     EEIVTRGTFSVDAAAQLEGKPSMMNRTESGGPGESAEASEPAGIRDMRSMPLPGDSRS
                     EVKNRVVSRVFVRQLTDVLEHYLLVKNALVLDDPDKAGQEASGLRQALDRTDMNLLTG
                     DDHVIWMDLSKRISDGLSVLSGSGDLERQRKGFQLVSDNLYTAIRTFGLADKTVYYQY
                     CPMYDNDRGGYWLSEDEAVRNPYFGNMMLTCGETKEKLSY"
     gene            complement(2162..3451)
                     /locus_tag="PLZ75_09670"
     CDS             complement(2162..3451)
                     /locus_tag="PLZ75_09670"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS72699.1"
                     /translation="MKISVKALLLIIILLPAPQLLRAQQEITFAYNPVSFTEYLSAVV
                     QGNIGYIAEKFSVSIAEAELKAARIFPDPEISLTYVNNEDARLQMGQAFESEISYPVS
                     LGNRRKAGISLAKSRHELSQMMLDLYFQNLRADAAMAYYACLRDQKKYEIHLSIYEQL
                     VKLASADSIRLNAGDATGLDAMQSSLEAMAYLTEVMQSLAEMQNSGMDLMQLQGKKFS
                     SSLDMASGDFPDPVFHFSIDGLLEKAFENRAELLIAVKSQEVSEKNLGLLRANRAFEF
                     NLEAGYSYNSIVLNEIAPAPAFNAVTAGLSFPIKFSGMNRGEIRAAEAEIRQNQELRN
                     ETELQIYTEMMQAYNNFITQAKKAEQFSKGLAETAGRILQGRIYAYQRGETGLVDVLN
                     AQRTFMDLQLSQLDTWFDYTAALIEVERAAGIWDLPE"
     gene            complement(3448..>6017)
                     /locus_tag="PLZ75_09675"
     CDS             complement(3448..>6017)
                     /locus_tag="PLZ75_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOS72700.1"
                     /translation="TQYQIEIEPDKLDKYDISLNDVIEGIERNNSNAGGSLMDRGDLS
                     YTIRGVGLIQDLEDLGRIVVSTTHGIPVYINDLGTLKYGNLERKGVFGYTDRTTDLSE
                     SVQGIVQMLRYENPSLVLKEIHKAVDELNEKILPEDVTIRVFLDRTHLVSATLNTVSH
                     TISYGVLLVVGVLIIFLGSWRSALVIAITIPLSLLIAFIFMYFFGIQANLLSLGAIDF
                     GILVEGAIVMLETVLRKREEEPDAMLSVDNIAKRSVEMGKPIFFAMLIIIIVYLPLFT
                     FERIERKMFAPMAYTVGFATLGALAVALIMIPGLSFTIYRKPRKVYHNKWLEKLTVLY
                     HKYNVKLLRKPRRVFLPLGLILALTVVLTFTVGKDFLPSLDEGSLWLQVQLPPGIALH
                     KSRAMADTLRNHLIKYEEVEYAMTQVGRDDEGTDPFSISHAECGVGLIPYREWKRGKR
                     KDDLIAELAADLERLPGYEIGFSQPIIDMVMDQIAGSHSDLAIKIFGEDLIEARRIAD
                     EIADLIREIKGAEDVSIEQEPPLPQLQIHAEREKIALHGLNVSDVSELIEVAIGGKAV
                     SQIFIGNRVYDVICRYTEKSRETPDRIGSLLLTTASGSRIPLSQVAEIKTTTGPGIIN
                     REENERFLTVRVNLRNRDISSFYSEASALIEKEVKYNHHDFRLKWSGQFENQRRAFTR
                     LILIVPLSLALMFLLLYWNFGNFRQAWLQMAMLPLSIFGGMVALNISGMSLNVSSAVG
                     FIALFGMFMMNGVLMITHINHLRKEGTELEKAITDGASHRLRQILMTSAVAILGLLPA
                     SLATNIGSDVQRPLATVIVYGLAFGTVITLATLPALYYLLELRFLKKNNNRVTTNPR"
BASE COUNT         1459 a         1427 c         1536 g         1595 t
ORIGIN      
        1 taactccctc accgcggatt ggtacgctgc aatgcagatg taccgggatg cggaacagag
       61 aatggatgtt tatgatgaac aatatgtgct tgcatcaaaa acactggaaa taatgctcaa
      121 aggcttttca gcctcttctg ttctgcttac ggacgtcctg cgtgttattc agcagacact
      181 ggattatgaa ctgatgcagg tcggggcact caccgatctg aacacagccg ctgcatggct
      241 gaaacgtttg atggcggtca gggaaaaccg ataaaaaaca aaagtatgaa gaggaaaata
      301 tattacagga tatttaaggg ccggtggccg gtttacattc tgcttttact cctgggtgtt
      361 ctgctgggca gggtgttctt tcattccggc ggacatgaag actctgccgg ccatcagcat
      421 gcaggggagg aaaaagttga gatctggaca tgttcaatgc acccgcagat aagaatgact
      481 gaaccaggga aatgtcctat ttgcgggatg gatcttatac cgcttgtttc cggcggtacg
      541 gttcaccatg atcatgatgc tgttcatctg tccagggaag ctgcggaact ggccaatgtg
      601 catacaacaa gggtagccgc cggagatccc gcaggggaga tcaggcttta cggaatggta
      661 cagctcgatg aacggctgct gcaaagccag gtctcccatg tttcaggccg tatagaaaaa
      721 ttgtttgtga actttaccgg tgaaactgtt actgccggac agaagctggc tgaaatttac
      781 tcacccgaac tggtaacggc ccagcaggaa ttgctggaga cagccggaac caggcagata
      841 cagcctctgc tttatgaggc atcaagggaa aaattacgtc atctgaaact aactgacgac
      901 cagatctcgg caattgagaa ttccggtacg ataataagct ctttcacggt agtgtcaaat
      961 acttcaggca ctgttatttc ccggagggta agcaccggag attacgtatc gcgcggcact
     1021 gttttgtatg acatagcagg cctgtcccgg gtatggctca tgctggatgc ctatgaatct
     1081 gatcttccgt tcatcaggac ggggcagcag gtaagcttca gggttgaggc agtaccggga
     1141 agggattttt caggaactgt tgcctttgtt gatcccctta tcgatccgct gacccgtgtg
     1201 gctggtgtaa gggtggatgt tggcaaccgt tccggaatgc tgaagccggg catgtttgca
     1261 accggaagtc ttgtatctga catggcagca taccgtaaca gtattgtggt tccgaaatca
     1321 gccgtgctgt ggaccgggaa aaggtcgata gtatatgtta aggatcctca ggcggatgaa
     1381 ccggttttca ggctgaggga agtgaaactg ggaccggcaa tcgggaatgc ctgggtaatc
     1441 accggcgggc tggcagaagg tgaggaaata gtgacccgcg gaacattcag tgtggatgcg
     1501 gccgcacagc tggagggcaa acccagtatg atgaaccgca ctgagagtgg cgggcccggt
     1561 gaatcagcgg aggcctcgga gccggccggc atccgtgaca tgcggagtat gcctcttccc
     1621 ggagacagcc ggagcgaagt gaaaaacagg gttgtcagcc gggttttcgt cagacaactt
     1681 acggatgtgc tggagcatta tcttctggtc aagaatgccc tggtgcttga tgatccggac
     1741 aaggccggac aggaggcttc cggtctccgg caggcactgg acaggacaga catgaacctg
     1801 ctgaccggcg atgatcatgt aatatggatg gatctttcga agagaatctc tgacgggctg
     1861 tcagtattgt ccggttccgg tgacctggaa aggcagcgga aaggctttca gcttgtgagc
     1921 gataatcttt acaccgccat ccgtactttc ggcctggcgg ataagacagt ctattaccag
     1981 tactgcccga tgtatgacaa tgacaggggc gggtactggc tcagtgagga tgaggcagta
     2041 cgtaatcctt atttcggtaa tatgatgctc acctgcgggg aaacaaaaga gaagctgagt
     2101 tactgaaaac ttccccggaa gattcagtgt aatatttgcg gatgcttcag atccgctgta
     2161 ttcactcggg aagatcccat atgccggcag ccctttccac ttcaataaga gcagcggtat
     2221 aatcaaacca ggtatccagc tggctgagct gcaggtccat gaatgtcctc tgtgcattta
     2281 aaacatcaac cagtcctgtt tctcccctct gataggcata gatgcgtccc tgaagtattc
     2341 tccccgcagt ttctgcaagg cccttgctga actgttcagc cttttttgcc tgggtgatga
     2401 agttattgta tgcctgcatc atctctgtgt atatctgcag ttcagtttcg ttgcgcaatt
     2461 cctgattctg ccggatttcg gcctcggcag cccttatttc cccccggttc attccggaga
     2521 acttgatcgg gaaggagaga ccggctgtta ctgcattgaa tgcgggagca ggagctatct
     2581 catttaaaac aattgagtta tatgaatatc ctgcttccag gttgaattca aacgccctgt
     2641 ttgccctgag caggccgagg tttttctccg atacttcctg ggatttgacc gctatcagca
     2701 attcagcgcg attctcaaat gccttttcca aaagaccgtc aatgctgaaa tggaataccg
     2761 gatcaggaaa atctcctgaa gccatatcga ggctgcttga aaactttttt ccctgaagct
     2821 gcataagatc catgccactg ttctgcattt ccgccaggct ttgcattact tcagtcaggt
     2881 aagccatggc ttcaagtgac gactgcattg catcgagccc ggtggcatcg cctgcattca
     2941 gtctgatact gtcggcagat gcaagcttaa ccagttgttc atagatggaa agatggatct
     3001 cgtatttttt ctgatcacgc aggcaggcgt aatatgccat ggcagcatct gcccggagat
     3061 tctgaaaata gaggtccagc atcatctgtg acagttcgtg ccttgatttt gccagagaaa
     3121 taccggcttt tctcctgttc ccgagactga cgggataaga tatttccgat tcgaaagcct
     3181 ggcccatctg cagacgtgca tcttcattat tgacgtatgt caggcttatt tcaggatcgg
     3241 gaaagatgcg tgccgccttc agctcagcct cagcaatgct cactgaaaat ttctcggcaa
     3301 tatatccgat gttcccctga accacggctg acaggtattc agtgaaggat acaggattat
     3361 atgcgaatgt gatttcctgc tgtgccctca gcagctgagg agccgggagg agaattatta
     3421 ttagcaaaag ggctttgaca ctgattttca tctgggattt gtggttactc tgttattgtt
     3481 cttttttaaa aacctcagtt ccaataaata gtaaagggcc ggaagggtgg ccagtgttat
     3541 cactgttccg aaggcaaggc cgtaaacaat cactgttgca agtggccgtt gaacatcgga
     3601 gccgatgttt gtggccaggg atgcaggcag cagtcccaga atggcaacag cggaggtcat
     3661 cagtatctgt ctcaggcgat gggaagcacc gtctgttatt gctttttcaa gttccgttcc
     3721 ttccttcctg agatggttta tgtgggtgat catgagcact ccgttcatca tgaacattcc
     3781 gaaaagtgca atgaatccta ctgcagagga gacattcagg ctcatccccg aaatatttaa
     3841 tgccaccata cccccaaata ttgagagagg cagcattgcc atctgcagcc aggcctgtct
     3901 gaaatttcca aaattccagt atagcagcag gaacatcaaa gccagtgaaa gaggaacgat
     3961 gagaatcagc ctggtgaatg cccttctctg gttttcaaac tggccgctcc atttcagacg
     4021 gaagtcatga tggttgtatt tcacttcctt ttcaatcagt gcactggcct cactgtaaaa
     4081 ggagctgatg tcgcggttcc gcagattgac ccttaccgta aggaaacgtt cattttcttc
     4141 cctgttgatg attccggggc ctgtagtggt ttttatttcc gctacctgtg acaggggtat
     4201 tctcgatccg gaggctgttg tgagaagcag tgaaccgata cggtcgggag tttccctgga
     4261 cttttccgta tacctgcaga tcacgtcata gaccctgttt cctatgaata tctgtgaaac
     4321 agcctttccg ccgattgcca cttcaataag ttctgacaca tcagatacgt tcagaccgtg
     4381 aagggcgatt ttttcacgtt ctgcatgtat ctggagctgg ggcaggggag gttcctgttc
     4441 aatgctgaca tcttcagctc cttttatttc cctgatgagg tcggcgattt catcggctat
     4501 tcgtctggct tcaatgagat cttcaccgaa aattttgatc gccagatcgc tgtgtgatcc
     4561 tgcaatctga tccataacca tgtcaatgat cggttgggaa aatcctatct catatcccgg
     4621 caatctttcc aggtctgcag ccagttccgc tatgaggtca tcctttcttt tcccccgttt
     4681 ccactcgcgg taggggataa gcccgacccc gcattctgca tgcgatattg aaaaggggtc
     4741 ggttccttca tcatccctcc ctacctgtgt cattgcatat tcaacttcct catattttat
     4801 cagatggttt ctgagtgtgt cagccatggc tctggacttg tgaagggcga ttcccggcgg
     4861 aagctgtacc tgcagccata atgaaccttc atccagggaa ggaagaaaat cttttcctac
     4921 ggtaaatgtc agtactacgg ttaaagcgag aattaatccc agtggcagga aaaccctccg
     4981 cggctttctg aggagcttca cattgtattt gtggtacagc actgtcagtt tctccagcca
     5041 tttattatgg taaaccttcc ggggcttccg gtaaattgtg aatgaaaggc cgggtatcat
     5101 gatcagtgcg acagccaggg ctcccagggt tgcaaacccc accgtatagg ccatgggtgc
     5161 aaacattttc ctttcgatcc tttcaaaggt aaacaggggc agataaacaa tgatgatgat
     5221 gagcattgca aagaagatcg gctttcccat ctccacgctt cttttggcaa tgttgtcaac
     5281 agacagcatg gcatccggct cttcctccct tttgcgcaga acagtctcaa gcataacaat
     5341 ggcaccttca acgagaatgc cgaaatcaat tgcacccagg gaaaggaggt ttgcctgtat
     5401 gccgaaaaaa tacatgaaaa tgaatgcaat cagaagagaa agcggtattg tgattgcaat
     5461 aaccagggca cttctccagc tgccgaggaa tattatcagg actccgacaa ccagcagaac
     5521 cccgtatgat attgtatgtg aaacggtatt cagtgtggct gaaaccagat gtgtcctgtc
     5581 aaggaaaacc cttattgtaa catcttcggg gaggattttc tcattcagct catctactgc
     5641 cttatgtatt tccttaagca ccagtgaggg gttttcatac cggagcattt gtacaattcc
     5701 ctgaacactt tccgacaggt cggttgtcct gtctgtgtag ccaaacactc ccttgcgttc
     5761 caggttgcca tacttgagtg ttccgaggtc gtttatgtat acggggatcc cgtgggttgt
     5821 gctgacaacg attctgccaa ggtcctccag atcctgtatc agtcccactc cccggatagt
     5881 gtagctcagg tcgccccggt ccatgaggct gccgccggca tttgagttgt tcctctcaat
     5941 tccttcaatg acatcattca ggctgatatc atatttgtca agcttgtcag gttctatttc
     6001 tatctggtac tgggttg
//
LOCUS       DAOSPX010000199         6000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12073_length_6000_cov_3.402691, whole genome shotgun sequence.
ACCESSION   DAOSPX010000199 DAOSPX010000000
VERSION     DAOSPX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12073_length_6000_cov_3.402691"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLZ75_09680"
     CDS             <1..600
                     /locus_tag="PLZ75_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOS72701.1"
                     /translation="VQELLSAAKIPVVRGTVVRTTGELAAVAGKTGFPLAVKVVGPVH
                     KSDVGGVTLNIRSPEHLEAEFRRMMKIRDVQAVLVQKMLSGTELFIGAKYEPKFGHVI
                     MCGLGGIFVEVLRDISSGLAPLTHDEARSMIRSLKGYSIIRGTRGQPGVDEGKFTEII
                     VRLSSLLRFATEIKEMDLNPLIGNPEAITVVDARVRIEK"
     gene            686..1114
                     /locus_tag="PLZ75_09685"
     CDS             686..1114
                     /locus_tag="PLZ75_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOS72702.1"
                     /translation="MLIQQLSVFLENKSGRLTEVLEVLGSKSSIRIKALTVADTTEFG
                     ILRLIVSDPEKARSILREQKFTVNMTEVISVVAPNEAKDYAKVLKILSDIDISVEYTY
                     AFTTGKSSVIILRCSNNEKAIRALKDNEMDLLMASDLYEI"
     gene            1139..2446
                     /locus_tag="PLZ75_09690"
     CDS             1139..2446
                     /locus_tag="PLZ75_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOS72703.1"
                     /translation="MNTNIWNRHFECMERDDLRYNQERRLRETVERVYFNVPYYRHKM
                     QEAGLGPESVNTLEDLRKLPFTTKDDLRDNYPFGLFAVPMSEIVRLHASSGTTGKPTV
                     VGYTRNDIATWSEVMARTLACAGAGRDDFIHIAYGYGLFTGGLGLHYGGEKIGASVIP
                     ISGGNTTRQLQLMRDLGSTILACTPSYALFLAEAIQENGVNRSDLKLKAGVLGAEPWT
                     ENMRKEIEKKLEIKAIDIYGLSEVIGPGVASECMVQNGLHVNEDHFYPEIIDPDTLEV
                     LPEGAMGELVFTTITKEGLPLIRYRTRDLTRLNYEKCECGRTFVRMEKCLGRSDDMLI
                     VRGVNLFPSQIETVLLEMKEIKPHYLIIVDRIHNLDTLELQVEVDEEFFQDKISQLQI
                     IRGRLQDRLENAIGLSINVTLVEPKTIERSEGKSQRVIDRRKF"
     gene            2462..3784
                     /locus_tag="PLZ75_09695"
     CDS             2462..3784
                     /locus_tag="PLZ75_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOS72704.1"
                     /translation="MHNIPIPSGIIERLACDNGIKSVGTASIREISLLAGLLEKETGI
                     PFIRMEMGVPGLATPSVAIEAEIDALQRGVSSKYPNVAGVRALKNEASRFIKLFLDTD
                     ISPESCIPTAGSMMGGLLSFLVANRNDRSKEGTLFLDPGFPVHKQQVKMLGHEYHSFD
                     LYDYRGEKLREKLESCLEGGKISSLLYSNPNNPAWICLNGEELRIIGELASKYDVIVI
                     EDLAYFGMDFRKDYSHPGVPPYQPSVAPYTDHYILLISSSKIFSYAGQRIGLMAISDK
                     LFNRSYPDLLRYYSSDRFGHALIFGALYGISAGVTHSVQYGLAALLRAVNDGTYNYRD
                     DVREYEEKASLMKKFFLSNGFDIVYDTDLDVPIADGFYFTISYPGMTGRALLQELLKY
                     GISAITLDITGSEREGLRACVSKVPRDLFPELEKRLLKFKEDHLVKRK"
     gene            3795..4019
                     /locus_tag="PLZ75_09700"
     CDS             3795..4019
                     /locus_tag="PLZ75_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS72705.1"
                     /translation="MAKIRGSIVIDIERCKGCEICMVTCPTGTIKMAGQVNHKGYFYA
                     WPANPELCIGCANCAVVCPDGVITVYKTKI"
     gene            4027..5096
                     /locus_tag="PLZ75_09705"
                     /pseudo
     CDS             4027..5096
                     /locus_tag="PLZ75_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
     gene            5100..5909
                     /locus_tag="PLZ75_09710"
     CDS             5100..5909
                     /locus_tag="PLZ75_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOS72706.1"
                     /translation="MAEVTGIKDYIRPENLVYSKTRLMTDNVLHYCPGCGHSTAHRIL
                     AEIIDEEGLQQDAIGISPVGCSVFLYNYIDIDWQEAAHGRAPALATAIKRLCPDKFVF
                     TYQGDGDLAAIGTSETIHSCNRGENFLIIFINNGIYGMTGGQMAPTTIPGMKSSTSPY
                     GRDTVRMGFPLKITDIVATLPGTYYVSRQSVHTALNVRRAKRALKKAVQYQSLNRGTC
                     FVEIVSNCPAGWKMTPVEANKWLEENMLPYYPLGDLKVPQPEKDLNPGSTD"
BASE COUNT         1726 a         1231 c         1604 g         1439 t
ORIGIN      
        1 gtccaggagc ttctcagtgc ggcaaagatt ccggtggtca gggggacagt tgtcagaaca
       61 acgggcgaac tggcagctgt agccggcaaa acgggatttc cccttgcagt caaggtggtt
      121 ggacccgtcc ataaatccga tgtcggaggg gtcactctga atatcagaag cccggagcat
      181 cttgaggcag aattcaggag gatgatgaag ataagggatg tgcaggcagt ccttgttcag
      241 aaaatgttaa gcggaacaga acttttcatt ggtgcaaagt atgaacccaa gtttggtcat
      301 gtgataatgt gcggactggg aggaattttt gtggaggttc tccgggatat ttcctccgga
      361 cttgcccctc tcactcacga tgaagcgcga tcaatgatcc ggagcctgaa aggctacagt
      421 ataattcgcg gaacaagagg ccagccggga gttgatgaag ggaaattcac cgaaataatt
      481 gtcagattgt ccagtctgct cagatttgca acagaaataa aggaaatgga tcttaatccg
      541 ctcatcggta atcctgaggc aattacggtg gttgatgcaa gggtcagaat agaaaaatga
      601 taagaatgac agggaatgca ttgatgaaaa aaggatataa ttattaaatt cggatttttt
      661 ttaatcatct tttcagacta agccgatgtt aattcaacag ttgtcagtat ttctggaaaa
      721 caaatcaggc cggctgactg aagtgcttga agttcttggc agcaagagca gtatcagaat
      781 caaggcgctg acagttgcgg acaccacgga attcgggata ttgcgcctga tagtatccga
      841 tcccgaaaag gccaggagca ttctcaggga gcagaagttc acggtgaaca tgacagaagt
      901 catttcggtt gttgcaccca atgaggcgaa ggactatgcc aaggtgctta aaatattgtc
      961 agatattgat ataagtgtgg aatacaccta tgcattcacc acagggaaaa gctcggttat
     1021 tatacttcgc tgcagcaaca atgaaaaggc aatcagggcc cttaaggata atgagatgga
     1081 cctgctgatg gccagcgatc tttatgaaat ctaatattaa aaattctgtt tttctccgat
     1141 gaatacgaat atctggaaca ggcactttga gtgcatggaa agggatgatc tccgttataa
     1201 tcaggaaaga aggctgaggg agactgtgga gcgggtgtat ttcaatgtgc cctattaccg
     1261 gcataagatg caggaagcag gacttggtcc cgaatccgtc aacactcttg aggatctgag
     1321 gaaactgcct ttcaccacca aggacgatct gagggataat taccctttcg gcctgtttgc
     1381 cgtgccgatg agcgagattg taaggcttca tgcttcttct ggtacaacag gcaagcctac
     1441 agtagtgggc tataccagga atgatattgc aacatggtcg gaagtcatgg cccgtactct
     1501 tgcatgtgca ggggcgggtc gtgatgattt tattcatatt gcctacggat acggcctgtt
     1561 tacgggaggc ctgggacttc attacggcgg ggagaagata ggagcatcgg tgattccgat
     1621 atcaggcggc aataccaccc gtcagttgca gcttatgcgc gatcttggct caaccattct
     1681 tgcctgtact ccttcctatg cactttttct ggctgaagcc attcaggaaa acggggtgaa
     1741 ccggagtgac ctgaaactga aggccggcgt tctcggagcc gagccatgga cagagaatat
     1801 gcggaaggaa atagagaaaa agcttgagat aaaagccatt gatatttacg gtttaagtga
     1861 ggtgatcgga ccgggtgtgg caagtgaatg tatggtacag aacggactgc atgtgaatga
     1921 ggatcatttc tatcccgaaa taatcgatcc ggataccctc gaggtcctcc cggaaggggc
     1981 tatgggtgaa ctggttttta ctaccattac aaaggagggg cttcctctaa ttcgttaccg
     2041 gacccgcgat ctgacgagac tgaattatga aaaatgcgag tgcggcagaa catttgtaag
     2101 aatggagaaa tgtctcggac ggagtgacga catgctgata gtgaggggag tcaatctctt
     2161 cccgtcacag attgagacag tgctgctgga aatgaaggaa ataaaaccac attatctgat
     2221 aatagttgac agaattcata atcttgacac tcttgaactt caggttgaag tggatgagga
     2281 attcttccag gacaagatca gtcagttgca gataatcagg ggcaggctgc aggacaggct
     2341 tgaaaatgcc attggtcttt caataaatgt aaccctggtt gaaccaaaaa caattgaaag
     2401 gagcgaaggc aagtcccaga gagtaattga cagacgtaag ttttgattaa attatagtta
     2461 catgcacaac ataccaattc cttccggaat tattgaaagg cttgcctgtg acaacggtat
     2521 aaaatcggtc ggaaccgctt ccataaggga aatatcctta ctggccggtc ttcttgaaaa
     2581 ggaaacggga atacctttta taaggatgga aatgggagtt ccgggcctgg ctaccccgtc
     2641 agttgccatt gaggcagaga tagacgcatt gcaaaggggt gtttcatcaa aatatcccaa
     2701 tgttgcaggt gtacgtgcat tgaaaaatga agcatcaagg ttcataaaac tgttccttga
     2761 tactgatatc agcccggaaa gctgtatccc gacagcaggt tcaatgatgg gagggctgct
     2821 aagctttctg gtggcaaaca ggaatgaccg cagcaaggaa ggcactcttt tcctcgatcc
     2881 ggggttccct gtacataagc agcaggttaa aatgctcggg catgagtatc attcctttga
     2941 tctttatgat taccggggtg agaagctcag ggaaaagctt gaatcctgcc tggaaggcgg
     3001 taaaatatcc tccctgctgt attccaatcc gaacaatccc gcctggatct gtcttaacgg
     3061 ggaggaactc cgtatcatag gcgagcttgc ctcaaaatac gatgtcatag tgatagagga
     3121 tctggcatat ttcggaatgg atttcagaaa ggattattca catccgggtg ttccgccgta
     3181 tcagccttcg gtggcaccct ataccgacca ctatatcctt cttatatcga gctccaaaat
     3241 attcagctat gccggtcaga ggatagggct gatggcaata tccgacaagc tgttcaacag
     3301 atcctacccc gatcttttaa gatattattc cagcgacaga ttcggtcatg ccctcatttt
     3361 cggagccttg tacggaatct ccgccggtgt tacccattcg gtacagtatg gcctggccgc
     3421 acttctcagg gccgtaaatg acgggacata caactaccgc gatgatgtca gggaatatga
     3481 ggagaaagct tccctgatga aaaagttctt cctgtcaaac ggctttgata ttgtatatga
     3541 tacagatctg gatgtgccta ttgccgacgg attctatttc acaatcagct atccgggcat
     3601 gacgggaagg gcattgcttc aggaacttct gaaatatggc atcagtgcca taacccttga
     3661 tataacgggc agtgagcggg aaggactgag agcatgtgtt tccaaggttc cgagggacct
     3721 gttccctgaa ctggagaaac gcctcttaaa attcaaagaa gatcatcttg tgaaaaggaa
     3781 ataaattcga ttcgatggca aagatcagag gaagtatagt aatagatatt gaacgctgca
     3841 agggatgtga gatctgcatg gttacctgcc cgacaggtac tataaaaatg gcgggacagg
     3901 tgaatcacaa gggttatttc tatgcctggc ctgcaaatcc tgaattatgt ataggatgtg
     3961 ccaactgtgc ggtggtatgc cctgacggtg tgataactgt ttataaaaca aaaatttaac
     4021 tttctgatgg agagagagct tagactgatg aaagggaatg aggcggttgc cgaagcggcg
     4081 atacgtgcgg gaactgacgg ttatttcggg tatccatcac tccccagagc gagatagtgg
     4141 aatatctgat ggaggcaaat cccgctgaaa cgacgggaat ggttgttctt caggctgaaa
     4201 gcgagattgc tgcaataaac atggtatacg gcggtgccgg ctgcgggaaa aaggttatga
     4261 caacatcttc ctcgcccgga ataagcctga tgcaggaagg aatcacctat atcgccggtg
     4321 cagagctgcc ctgccttatt gtgaacatag tcaggggcgg tcccggactc ggaacgattc
     4381 agccggcaca gtcagactat ttccaggcgg taaagggcgg gggacacggg gattatcacc
     4441 tgatagtgct tgctcctgca tcggttcagg aaatggccga ttttgtggaa ctgggattcg
     4501 aacttgcctt caggtaccgg aatccggtga tgatgctttc cgatggcgcg ctaggacaga
     4561 tgatggagaa ggtatatctc aaccctcaga aggaaagatc cggaatatca cctgactggg
     4621 ctaccacggg aaaacccccg ggaagggaaa ggaatatcat tacttcactg gaactggatt
     4681 ccgcgaagca ggaattgttc aacctgaagc tcatcgaaaa atacaatgaa atacgcgaaa
     4741 aggaagtgag atttgaggaa tatcagtgtg aggatgctga atatctgttt gtcgcttacg
     4801 gaacaagcgc caggatctgc cagaaatcag tacagctcgc acgtaatgag ggaataaggg
     4861 cagggcttct ccggccggtc actcttttcc ccttccccga aaagcagatc agcaggctgg
     4921 cagaaaaggt gaaaataatt ctgtcagtgg aaatgagtgc gggtcagatg attgaagatg
     4981 ttatactggc cgttggggga aaggtgccgg tgtttcattt cggcagaatg ggaggaatgg
     5041 tacagacacc cgaagaggtg ctggattttt tgaagttaaa aataaaggag gcttaggaaa
     5101 tggcagaagt aacaggaata aaggattata tcaggcctga aaatctcgta tacagcaaaa
     5161 cccggcttat gacggacaat gttcttcatt actgtccggg atgcggtcac agcacggcac
     5221 acaggatact ggccgaaatc attgatgagg aagggcttca gcaggatgca ataggcattt
     5281 ccccggtggg atgctctgtg tttctgtata attacattga tattgactgg caggaagcgg
     5341 ctcacggaag ggctccggca cttgcaactg caatcaagag actgtgcccg gacaagtttg
     5401 tgtttaccta ccagggcgac ggtgaccttg ctgcaatagg aacctccgaa acaatacatt
     5461 cctgcaacag gggagagaat tttctcatta tcttcataaa caacgggata tatggcatga
     5521 ccggcggaca gatggcaccc accacaattc ccggaatgaa atcatccact tcaccttacg
     5581 gaagggatac agtgcggatg ggatttccgc tgaaaataac tgatattgtt gcaacactcc
     5641 cgggtactta ttatgtaagc cggcaaagtg ttcacacagc gcttaatgta agaagggcta
     5701 agcgggctct gaaaaaggct gttcagtatc aaagccttaa caggggaacc tgttttgttg
     5761 aaatagtatc aaactgtccg gcaggctgga aaatgacacc ggttgaagca aataaatggc
     5821 ttgaggaaaa catgctcccc tactatcccc tcggagacct caaggtcccg cagccggaaa
     5881 aggatctgaa tcccggcagc acggactgac ggaataaatt ggctgaaagg ataaatagac
     5941 tgaaaggata aaatgacaga agaaataata attgcaggtt ttggcggaca gggaatcctg
//
LOCUS       DAOSPX010000200         5986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12104_length_5986_cov_3.483392, whole genome shotgun sequence.
ACCESSION   DAOSPX010000200 DAOSPX010000000
VERSION     DAOSPX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12104_length_5986_cov_3.483392"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLZ75_09715"
     CDS             <1..424
                     /locus_tag="PLZ75_09715"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOS72707.1"
                     /translation="AAGADSIMIGSLFAGVEESPGETIIYSGRKFKAYRGMGSLEAMQ
                     QGSKDRYFQDMEDDILKLVPEGIAARVPYKGLLSEVIFQMTGGLRAGMGYVGAKNLKV
                     LKKEARFVRITGSGIIESHPHDVTITRESPNYSRKVFE"
     gene            444..2411
                     /locus_tag="PLZ75_09720"
     CDS             444..2411
                     /locus_tag="PLZ75_09720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS72708.1"
                     /translation="MKIRFLLLFTAASLLICPTIYCQALNDKTLMTIAGQEVTAGEFI
                     RMLKKSGDPGAKPDAAGYLREFINFKLKVADAINEGIDTTAAFRNELNGYRNQLAQNY
                     LSDPDTKEKLLRAAYDRSLTEINAWHILISCSGDSRPEDTLLAWQKAMEVRKRIISGE
                     SFEQAARESSDDPSAEINGGNLGYFTVFQMISPFEDAAYALNKGDISLPVRTPYGYHI
                     IKVADRRPASGKILTAHIMKMSPPGASEEAAAEAEKSINEIYDRLREGASFSELAEKY
                     SDDSGSASKGGQLNWFGTGEIISDYAEAAFALKDTGSYSLPVRSPYGWHIIKLLDRKA
                     HGTYEEMLPFLESRINPSQISALSKKTFVSNLQKEYNFRLDTAAYNWFLGHTDTLIIR
                     GLSKYNRKEMPAGNLFTFAGRQFSTEEFALFIENKGAGTGMKATAAFVGESLDNLVSD
                     QIIKYEDSVLEEKYPGFKYLMKEFHDGILLFDISERKVWNRVQEDSTGLFRYYEKHRQ
                     NYPGKRKMEVKTYTLSLRKGAGKLRKAYRGFSGMADCDKRMLERFNTATDSLLSISEA
                     VLSEGDPGTYRLKWKEGVVRTKSNNYPSLTVINRIIEPSPLPFEEVRTEIMAAYQDFL
                     ESEWLGQLREKYPVSIDTKVLEEVKMQMDNE"
     gene            2431..3261
                     /locus_tag="PLZ75_09725"
     CDS             2431..3261
                     /locus_tag="PLZ75_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72709.1"
                     /translation="MIILIAGGCKNNNSKRVPLAKAGKSTLYLDQVTGLVPAGTPSTD
                     STALVKNYINSWAKRELMYQKAEENITADLMKDIDNQVREARMNLVIYQYQRQMMLQK
                     MDTTISDTELENYYLNNKGSFVLNYNIVKALLIKLPQETPGLARIRNLARSNNQANLH
                     QLESLCYQFAEKYDDFKDEWVPLNQLMAEFPYVTNNQENFLRNNTFYERQSADSAYVY
                     MLSIRDYRLRSSVAPFEYVRDDIKRIIWTNRRLEFIQSLENGIYNDALKKNIFTLLTK
                     "
     gene            3268..4626
                     /locus_tag="PLZ75_09730"
     CDS             3268..4626
                     /locus_tag="PLZ75_09730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS72710.1"
                     /translation="MRRYSGLFLIVAVFWLSGLIPGRAQVLVESVAAIVGNEVIYLSD
                     IENMVLDLRSSGNKRPVEELRCNVLRELLIQKLFLDQARLDSIEVTDDMVEGELNARL
                     NDAIRRSGSEEALEKYFGKSMIEIRRDIRKALIDQEIVSQVQGTVAGNITITPNELKK
                     FFNNIPADSLPVVPARVQISIIQLDPPDNEENKAEARQKLLNIRGEILAGKSFSALAV
                     LYSEDPGTATRGGELGFMLRGELEKPYADEAFSLTKNTVSRIIETRYGFHIIQMIDRN
                     GDLINTRHILIRPKVKPEQAARALHRLDSLANLIRKDSLKFENTAIRYSTHKDSRING
                     GKYVKTNPSERDTWFTLEELNPQMYVQVRDLKIGEISEAFRTVDENNNEVFRIVRLDN
                     EVPAHRANLKDDYQSLSNAALMSKRTKAYDKWIRKKIEVTYIKISDEFRSCGFLDEGW
                     LR"
     gene            4614..>5986
                     /locus_tag="PLZ75_09735"
     CDS             4614..>5986
                     /locus_tag="PLZ75_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOS72711.1"
                     /translation="MAKVNLFQKIRKLIPAGQVVYAVLIFIVTIAAALQPQEISGNKP
                     GKRKIILRHADEDIILKDRETGKDWHRLLGSVSLEHNQISMFCDSAHFYPDKNQVKAY
                     GSIHIEQGDTLDIYGNYLFYDGITEIAVLTGNAELVDKETHLYTNSITYDVKSEIAQY
                     RDSGRIINGDNTLTSKIGRYYTATRIFNFKNDVKITNPDYIMTADTMDYNTETKTAFF
                     TGPSEMKGDSIYIYCERGWYDTGNKISRIWKNALLDNRKQIIRGDSLYYEENNGYGLA
                     FGNASITDTSHQVSVAGDYAWYYREPERFMITDSAVFIQISDKDSLYLHGDTIRSVTL
                     TDSLGISFRLMSAYYGCRIFSRDMQAKCDSLSYSFRDSVIRFYNEPVLWAEENQMTSD
                     SMALFTKNRKADRMELFSSAFVASQVDTMRYNQIRGRSLTGYFRDNELYKIEINGNGE
                     SLYYLGEG"
BASE COUNT         1902 a         1288 c         1455 g         1341 t
ORIGIN      
        1 tgccgcagga gcagattcaa taatgatagg ttctcttttc gcgggcgtgg aggagtctcc
       61 gggtgaaacg ataatttaca gcggacgtaa attcaaggct tacaggggaa tgggttcgct
      121 tgaagcaatg cagcagggct caaaggacag atatttccag gatatggaag acgatattct
      181 gaagctggtg cccgagggaa tagctgcaag ggttccctat aaaggcctgc tctcggaagt
      241 catttttcag atgacgggag gattaagggc tgggatggga tacgtgggag caaaaaacct
      301 taaggtactt aagaaggaag ccagattcgt aaggataacc ggttcaggca taattgaaag
      361 ccatccccat gatgttacca taacaaggga atcacctaat tacagcagaa aagttttcga
      421 ataaaccgat tcattaaata ctgatgaaaa tcagattttt gttgcttttc acggctgcct
      481 ccttactcat ctgccccaca atttactgcc aggcactgaa tgacaaaact cttatgacaa
      541 ttgccggaca ggaggttaca gccggggaat tcatccggat gctcaaaaaa agcggtgacc
      601 cgggtgcaaa gcctgatgcc gccggatacc tccgggagtt tattaatttc aaactgaagg
      661 tggctgacgc cataaacgaa gggattgaca ccactgctgc attccggaat gaactgaacg
      721 gatacaggaa tcagctggca caaaattacc tcagcgatcc tgatacaaag gagaaactgc
      781 tcagggctgc ttacgacaga tccctgacgg aaataaatgc atggcatatt cttatcagct
      841 gttcggggga ttcccggcct gaggatacac ttcttgcctg gcagaaagca atggaggtca
      901 ggaaacggat aatcagcgga gaatcctttg aacaggcagc cagggaatca tcagatgacc
      961 cttcagcgga aataaacggt ggcaatctgg gctatttcac ggtatttcag atgatcagcc
     1021 cctttgagga tgctgcatat gcactgaaca agggtgatat atcattgcct gtcagaacac
     1081 cctacggata tcatatcata aaggttgcag acaggcgtcc ggcctcagga aagatactga
     1141 ccgcacatat catgaaaatg tcacctcccg gggcatcgga agaagctgcc gcagaggccg
     1201 aaaaatcaat aaatgagatc tatgacaggc tcagggaggg tgcttcattc agtgaacttg
     1261 cagaaaagta ttccgacgac agcggatctg cttcaaaggg aggacagctc aactggttcg
     1321 gcacaggaga aataataagc gattacgctg aggctgcctt tgcattaaaa gacacaggaa
     1381 gttacagcct gcccgtcaga tcgccatacg gatggcatat aataaaactt ctcgacagga
     1441 aagctcatgg aacatacgaa gaaatgctgc cattcctgga gagcaggata aatccctcac
     1501 agattagtgc cctgagtaaa aaaaccttcg tcagcaatct tcaaaaagaa tacaatttca
     1561 ggcttgacac tgctgcctac aactggtttc tcggccatac ggacacgctg ataataaggg
     1621 gcctgtcaaa gtataacaga aaagagatgc ctgccgggaa cctctttacc tttgccggca
     1681 gacagttttc aacggaggaa tttgctctgt tcatcgaaaa taaaggagca ggaacaggca
     1741 tgaaagcaac ggcagctttc gttggcgaat cactggataa tctggtttca gaccagatta
     1801 taaaatatga agattcggtc ctggaggaaa aatatcccgg atttaagtat cttatgaagg
     1861 aatttcacga cggaatcctg ttgtttgata tttcggaaag gaaagtctgg aacagggtgc
     1921 aggaagacag caccggcctg ttcagatact atgaaaaaca caggcagaac taccccggca
     1981 aacgtaaaat ggaagtaaaa acatatactc tcagcctcag gaagggtgca gggaaactcc
     2041 ggaaagcata caggggcttt tccggaatgg cggattgtga caaacggatg ctggaaaggt
     2101 tcaacacggc aactgactct cttctctcaa ttagcgaagc tgtcctgtct gaaggcgatc
     2161 ccgggacata caggctgaaa tggaaggaag gagttgtcag gacgaaaagc aacaattacc
     2221 cgtcactgac cgtaataaac aggataattg aaccttctcc cctgcccttt gaagaagtga
     2281 ggactgagat aatggcagcc taccaggatt tcctggaaag cgaatggctc gggcagctaa
     2341 gggagaaata ccctgtcagt attgatacaa aggttcttga agaggtgaaa atgcagatgg
     2401 acaatgaata gattattttt aatattattt atgatcatcc tgatagccgg aggttgtaaa
     2461 aacaacaatt caaaaagggt tcctctggca aaagcgggaa aatcaactct ctatctcgat
     2521 caggttaccg gactggttcc ggcaggcaca ccatccaccg acagcactgc acttgtaaaa
     2581 aattacatca acagctgggc aaaacgcgaa ctgatgtacc agaaggccga ggagaacatt
     2641 acagccgacc tgatgaagga tatagacaac caggtgaggg aggccaggat gaatctcgtc
     2701 atttatcagt atcagaggca gatgatgctc cagaaaatgg ataccacaat aagtgacacg
     2761 gagcttgaaa actactatct gaacaacaag ggaagctttg tactgaacta taatatagtg
     2821 aaggcccttc tgatcaagct tccccaggaa acacccggac tggcaagaat aagaaacctt
     2881 gcccgttcaa acaatcaggc gaatctccat cagcttgaat ccctctgcta ccagtttgcg
     2941 gaaaaatatg atgacttcaa ggacgagtgg gtgcccctga atcaattaat ggcggaattc
     3001 ccctatgtta caaacaacca ggaaaatttt ctgcggaata acacgtttta tgagaggcaa
     3061 agcgcggatt cggcctatgt ttacatgctg tcaatacgcg attacaggct caggtcatca
     3121 gttgcaccct ttgaatatgt aagggatgat ataaaaagga tcatatggac caaccggaga
     3181 ctagaattca tacagtcgct tgaaaacgga atatataatg atgcactgaa aaagaacatt
     3241 ttcaccttac taactaaata agctattatg agaagatatt cagggttatt cctgattgtg
     3301 gctgtattct ggctttcggg cctcattccc ggccgggcac aggttctcgt tgaatctgtt
     3361 gcggcaattg ttggaaatga agtaatctat ctttcagata ttgaaaatat ggttctcgat
     3421 ctcagaagca gcggaaacaa aagaccggtt gaggaactgc gctgcaatgt gctcagggaa
     3481 ctgctcattc agaaactctt tctcgaccag gcccgcctgg acagtattga ggtaacggac
     3541 gacatggtgg aaggggaact gaatgcaaga ctgaatgatg caataaggag atcgggaagc
     3601 gaagaggcac ttgaaaaata tttcgggaaa agtatgattg agatacggcg tgatatccgc
     3661 aaggcactga ttgaccagga gatagtcagc caggtacagg gtacggttgc aggaaatatc
     3721 acaataactc caaatgaact gaaaaaattc ttcaataata tccccgccga ctcccttcct
     3781 gttgtccccg ccagggtgca gatcagcata attcagcttg atcctcctga caatgaggaa
     3841 aacaaggctg aggcccggca gaaactcctg aatatcaggg gggaaatact tgcaggcaag
     3901 agtttctccg ctttagcggt tctgtattcc gaggatcccg ggacagccac gagaggaggt
     3961 gaacttggct tcatgctcag gggcgagctt gaaaaaccct atgccgatga ggcattcagc
     4021 cttacaaaaa acaccgtttc aagaatcatt gaaacgagat acggattcca catcatccag
     4081 atgattgacc gcaacggcga ccttattaat acaaggcata ttctcataag gccgaaagta
     4141 aaacccgaac aggcggcaag ggcattacac agactcgatt ctcttgcaaa tcttatcagg
     4201 aaagacagtc tgaaattcga aaatactgcc ataagatatt caacgcataa ggacagcagg
     4261 ataaacggcg ggaaatacgt taagaccaat ccttcagaaa gggatacctg gtttacgctt
     4321 gaagagctta acccgcaaat gtacgtacag gtgagggatc tcaaaatagg tgaaatatcc
     4381 gaagccttcc gtaccgttga tgagaacaac aatgaggtgt tcaggattgt caggctggat
     4441 aatgaagtgc ctgcacacag ggccaacctg aaggatgatt atcagagcct gagcaacgca
     4501 gcattgatga gcaaacgtac aaaagcttat gataagtgga tcagaaaaaa aattgaagtc
     4561 acatatataa aaataagcga cgaattcagg tcgtgcggct ttcttgatga gggatggcta
     4621 aggtgaatct ttttcagaaa ataagaaagt taatccctgc cggacaggtc gtatatgccg
     4681 tgcttatttt catagttaca atagcagccg cacttcagcc acaggaaatc tcagggaata
     4741 aacccggcaa aagaaaaatc atactcagac atgccgatga agacataatc ctgaaagaca
     4801 gggagaccgg caaagactgg cacaggctgc tgggcagcgt ttcccttgaa cacaaccaga
     4861 tttcaatgtt ctgtgacagt gcacatttct atcccgacaa aaaccaggtt aaggcttacg
     4921 gcagcataca catcgagcag ggtgacaccc tcgatattta cggaaattat ctcttttatg
     4981 acggcattac cgaaatagca gtactcaccg gaaatgcgga acttgttgac aaggaaaccc
     5041 acctttatac aaactccata acctatgatg taaaatcaga aattgcacaa tacagggaca
     5101 gcggcaggat catcaacggg gataataccc tgacaagcaa gatcggaaga tactatactg
     5161 ccaccaggat cttcaatttc aaaaacgacg tgaagattac aaatccggat tatatcatga
     5221 ccgccgatac catggattac aataccgaaa ccaaaactgc attttttacc gggccgtcgg
     5281 aaatgaaggg cgacagtata tatatctact gcgaaagggg ctggtatgat accggcaaca
     5341 aaatatcgcg aatctggaaa aatgcattgc ttgacaacag gaaacagatc atcaggggcg
     5401 attctcttta ctatgaggaa aataacggat acggccttgc attcggaaat gcaagcatca
     5461 cggatacaag tcaccaggta tccgttgccg gtgattatgc ctggtattac agggaacccg
     5521 aacgcttcat gattaccgac agcgcagtgt ttatccagat atcggacaag gattcccttt
     5581 atcttcatgg tgatacgatc agatcggtca ctcttaccga cagcctggga ataagcttca
     5641 ggcttatgtc ggcttattac ggatgcagga tattcagcag ggacatgcag gccaaatgcg
     5701 attcactgtc atattccttc agggattccg tgataaggtt ctacaatgaa ccggtactct
     5761 gggcagagga aaaccagatg acctccgatt ccatggccct gttcacgaaa aacaggaagg
     5821 ccgacaggat ggaactgttc agttccgctt ttgtggcaag ccaggtggat acgatgagat
     5881 acaatcagat caggggcaga tccctgacgg gatatttcag ggataatgag ctttacaaga
     5941 tcgaaatcaa cggaaacggg gagtctctct attacctggg agaagg
//
LOCUS       DAOSPX010000201         5984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12112_length_5984_cov_4.075392, whole genome shotgun sequence.
ACCESSION   DAOSPX010000201 DAOSPX010000000
VERSION     DAOSPX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12112_length_5984_cov_4.075392"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2552)
                     /locus_tag="PLZ75_09740"
     CDS             complement(<1..2552)
                     /locus_tag="PLZ75_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS72712.1"
                     /translation="MKKTELIGTDIRKVPVKHFPSMMKMFLILLLTSLFFVSQAAAQQ
                     KKQLSGTVADPSGEPLIGASIIEKGTTNGTLTNINGMFSLQVEENAVIQVSYIGFLSQ
                     EISTAGRTSLTITLLEDTRSLEEVVVVGYGTQKKANLTGAVDQVGEKVFVGRPMPNVT
                     QGLQGVVPNLNIALADGKPSRTASYQIRGVTSIGQGGSALVIIDGVEGDPAMLNPNDI
                     ASISVLKDASSAAIYGARGAFGVVLITTKNPEKGRTSVTYNASVVAKSPTRIPDMVTD
                     ANLYATMFVEAYSSYYDYARTPSTFHKAVPYTVDWHNEMARRPPGSGKPEVEIGPGGN
                     YQYFANNNWYEMLYKDRAFGHEHNIAIQGGTDRADFILSSRYYNQGGIFQYNSDDYSM
                     LNIRAKGSAQIFNWLKVENNVAITQSNYFNPLTVADGNVWYGLESEAEPMSPMFNPDG
                     TLTMAAAYSVGDMWYGKSGINTRKMLLHNTTSFTASFLDNAFRLKGDISFRNPYNRET
                     TKRVPVPYSPAPDVIAYLGSGTNDFGEANTSTNYMATNIYGEYEKTFAETHYFKGMLG
                     YNYEQSLFNSIFTQRNGLIFEDVNNINLTNGSGITISASHEKWRIAGGFFRFNYGYKD
                     RYLIEVNGRLDGSTKFPVDQQWGFFPSISAGWRLSEEPFWNLNNIFSDVKLRASYGSL
                     GVGNIASYTFQELFTISTMGRLIQGTRQQKTSAPPVLPDGLTWETSTTANLGLDFGVL
                     KGRLRFAGDIYTRKTKDMFAVGIDLPATFGATAPKGNYADMKTNGWEMTLSWRNDHTL
                     AGKPFSYDVRVTLADYVSKITKYTNPEKELGAYWDARANYYEGMTIGEIWGYV"
     gene            complement(3038..5218)
                     /locus_tag="PLZ75_09745"
     CDS             complement(3038..5218)
                     /locus_tag="PLZ75_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS72713.1"
                     /translation="MKTKTFIVFLSFFLLVQCSTNEKRLKPPLAPVKPVTDEYFGVKI
                     TDPYRYMENLQDTTVRQWMKQQSDYSRKILNSIPGRQRMINMMVDFDKRKSSTISSLR
                     IIDNDRYFYLKMTPADETGKLYYRDGFEGKEILMFDPKTYSQDTTKKYVISSVNPSID
                     GSKVAIDIAPNGSESAVLLTIETETLYLYPEKIDRRFGGFPVSWLADNNSFLYNRLQS
                     DDVHDANREKDSKTYLHIVGKDPSTDMEVFSRSNNPELGIKPEELPFVFYDKDSKFLF
                     GQIVTVDNRINLYYAPSSDLGKQKIAWLHLIKPEDEVYDFFTTDKDFYLYTPKNAPNF
                     KILKTSFEKPDLKNAEVIVSEDPERKMTNYSITSEGLLYTLSDNGIKETLFFLPKAEK
                     TAKEIELPFVAGTVRLSSKGYKFADCWAGLTGWTSDYQRFRYIPQKNEFKLENLSSVA
                     EYPEYKDLTVEELMIPSHDGVNVPLSLIYNKSIKKTGNNPVLFYGYGAYGMSITPGFS
                     SDLLLWTHEGGILAIPHVRGGGELGEKWYRDGYKTTKPNTWKDLIACAEYLVKEQYTS
                     PQKIAINSASAGGILVGRAMTERPDLFAVSIPQVGCLNTVRAEESPNGPVNTPEFGTV
                     KDSVECMALIEMDSYIHVEDGEKYPSTLITAGMNDPRVIAWQPAKFAARLQAANKSDK
                     PILFLVDYEAGHGIGNTKTKIFEEFADVFSFALWQTGHPDYQIK"
     gene            5557..5682
                     /locus_tag="PLZ75_09750"
     CDS             5557..5682
                     /locus_tag="PLZ75_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72714.1"
                     /translation="MRQGFFIEDKKSTEISNHELIRDMYVKVLKFGKVIMGSDMA"
BASE COUNT         1609 a         1234 c         1243 g         1898 t
ORIGIN      
        1 acatagcccc agatttcgcc tatagtcatg ccttcataat agtttgcccg tgcatcccag
       61 tatgctccaa gttccttctc ggggttggtg tatttggtaa ttttcgacac atagtcagcc
      121 agggtaactc tgacgtcata actaaagggt ttgccggcaa gggtgtgatc atttcgccag
      181 ctgagtgtca tttcccatcc gttggttttc atatccgcat agtttccctt gggtgcggtt
      241 gctccgaaag tagccggaag gtcaattccc acggcaaaca tatcctttgt cttgcgggta
      301 tagatatcac cggcaaagcg taacctgcct ttgagtactc cgaagtcaag gcccaggttt
      361 gcagttgttg aggtttccca tgtcagtccg tcgggcagta ccgggggagc cgaggttttc
      421 tgctgacgtg ttccctgtat cagtcttccc attgtactga tggtgaaaag ttcctggaat
      481 gtataggaag caatgttccc gactcccagt gaaccatatg atgccctgag ttttacatcg
      541 gagaatatat tgttcaggtt ccagaagggt tcttctgaga gacgccatcc tgcagaaatt
      601 gacgggaaaa agccccactg ctggtcaacc ggaaattttg ttgaaccatc aagacggccg
      661 tttacctcaa tcaggtatct gtccttatat ccatagttaa aacggaaaaa cccaccggca
      721 attctccact tttcgtggct ggcactgatg gtgatcccgc ttccgttggt caggttaata
      781 ttgtttacat cttcaaatat cagaccgttg cgttgagtga atatgctgtt gaacaatgac
      841 tgttcatagt tgtatccaag catgcccttg aaataatggg tttcggcaaa ggttttttcg
      901 tattcaccat aaatgttggt agccatatag tttgttgacg tatttgcctc tccgaaatcg
      961 tttgtgccgg atccgaggta tgcaatcaca tccggtgcag ggctgtaggg cacgggaaca
     1021 cgtttggtgg tttccctgtt ataggggttg cggaaggaaa tgtcaccttt cagcctgaag
     1081 gcattgtcca ggaacgaagc agtaaaagat gtggtattat gaagaagcat ttttctcgtg
     1141 ttgattccgc ttttcccgta ccacatgtct ccaacgctgt aggctgcggc cattgtaagt
     1201 gttccgtcgg ggttgaacat aggactcatg ggttctgcct cactttcaag tccgtaccac
     1261 acatttccgt cggctaccgt cagcgggttg aaataatttg actgcgttat cgccacattg
     1321 ttttcaacct tcagccagtt aaaaatctgt gcggatccct ttgcccgtat attcagcatt
     1381 gaatagtcat cgctgttata ctgaaatatg ccgccctggt tataatatct tgaagaaaga
     1441 ataaagtcgg ccctgtctgt gcctccctga atggcaatat tgtgttcatg gccgaaggca
     1501 cggtccttat acagcatttc ataccagttg ttattggcaa aatactggta attcccgccc
     1561 gggccgattt caacttcggg ctttccggat cccggaggtc ttcttgccat ttcattatgc
     1621 cagtcaacag tatagggaac agccttatga aatgttgaag gagttctggc atagtcataa
     1681 taggatgaat atgcttccac aaacattgtg gcataaagat tcgcatcggt taccatatcg
     1741 ggtatccgtg taggtgattt cgctacgacg gaagcattat aggtaacgga agtgcggcct
     1801 ttttccggat tcttggttgt gataagcacg acgccgaagg ctcccctggc cccgtaaata
     1861 gcggccgagg aagcatcctt caggactgag atgcttgcta tgtcattggg attgagcatg
     1921 gcagggtcgc cctccacacc atcgataatt accagtgcgc ttcctccctg tccgatggaa
     1981 gtaacccccc ggatctggta gcttgctgtt ctgcttggtt taccgtcagc cagagcaata
     2041 ttgaggttcg gaacaactcc ctgcagtccc tgggtcacgt tgggcatggg acggcccaca
     2101 aacacctttt caccaacctg atcgaccgct ccggtaaggt tggccttttt ctgtgttccg
     2161 taacccacaa ccaccacttc ttcaagtgag cgggtatctt ccagcagggt gatggtcagg
     2221 gaggttcttc cggctgtact gatctcctgg ctcagaaagc cgatatagga aacctgaatg
     2281 actgcatttt cttcaacctg cagcgaaaac ataccattga tgtttgtcag ggtaccgttg
     2341 gttgtacctt tttcaatgat acttgcacca ataagaggtt ccccggatgg atcagccaca
     2401 gttccggaaa gctgtttctt ctgctgcgcg gccgcctgtg acacaaagaa caggctggta
     2461 agcagcagga tgagaaacat tttcatcatt gacggaaaat gttttaccgg tacttttctt
     2521 atgtcagtac caattaattc ggttttcttc atgcttttat actgtttagg ttaatttctc
     2581 aaagacaatt tcaattacat tgtttttaat catttcccat aggcagaaaa ttaatggtta
     2641 atacagtaat taattgatta tacagtaatt ataataaaat tccctctctc cggcattgta
     2701 attgtgttat taacgaacca ataagggcca aattttgaat taaagtatta ttctacaata
     2761 aacagcttag tcatagcgga ttaaacaaag ttatctgatc cactcctcac aaatttaata
     2821 ttttaatgtt tggaagcagt ataaatcccg gaaaaattcg tctgaaaacc gcatactata
     2881 agtagactgt ttatatctcg catgccgtgc atacggtgcg ggcgtgtaac aaaaagcccc
     2941 ggtgcgaaag ctgcggggct ttttgtaaac aagaggtttg aaatatcgaa tcaatgttta
     3001 ataagggaca tggttagcat tatcttgcta gaacagttta tttgatttga taatctggat
     3061 gaccagtttg ccagagagca aagctgaata catcagcgaa ttcttcaaag atttttgttt
     3121 ttgtattgcc tattccatgt ccagcttcat aatcaactag aaaaagaatc ggtttatcag
     3181 atttattagc agcttgtaac ctggctgcaa attttgccgg ttgccaggca attaccctgg
     3241 gatcgttcat tcctgctgta attaatgttg atggatactt ttcaccatct tctacatgaa
     3301 tataagaatc catctcaata agtgccatac attcaacaga atccttaacc gtcccgaatt
     3361 caggtgtatt cacaggacca tttggtgact cttctgcccg aactgtgttc aggcaaccga
     3421 cctgaggaat tgagacagca aacaagtctg gtctttcagt cattgcccgc cctaccagta
     3481 taccaccggc actggctgaa ttaatggcta ttttttgagg tgaagtatat tgctctttaa
     3541 ccagatactc ggcacaggca atcagatctt tccaggtatt aggttttgtg gttttgtacc
     3601 cgtctctgta ccatttttca ccaagctctc cgccacctct cacatgaggt attgcaagga
     3661 tccctccttc atgggtccaa agcaataaat cggagctaaa tccgggagtt attgacattc
     3721 cgtaggctcc atatccatag aagagaaccg gattatttcc agttttcttt atacttttat
     3781 tgtatatcaa tgaaagcgga acattaaccc cgtcatgtga tggaatcata agttcttcaa
     3841 cggttagatc tttatactca ggatactctg caacagatga gagattttca agcttaaatt
     3901 catttttttg tggaatatag cggaaccgct ggtaatcgct tgtccatcct gtcagaccag
     3961 cccagcagtc agcgaattta taaccttttg aacttaatct tacagttccg gcaacaaacg
     4021 gtagttcaat ctcttttgct gttttttcag ctttaggaag gaagaagagg gtttccttaa
     4081 ttccgttatc agacagagta tataataatc cctcgctggt gatgctataa tttgtcattt
     4141 ttctctcagg gtcttcagaa acgataactt cggcattttt taaatcaggt ttttcaaagg
     4201 atgttttcag aattttaaaa tttggcgcat ttttaggtgt atataaataa aagtctttat
     4261 cagttgtaaa gaaatcatat acttcatctt cgggtttaat caaatgcagc catgcaattt
     4321 tttgttttcc cagatcagat gatggagcat aatacaggtt tattctatta tcaacagtta
     4381 ctatttgtcc aaataaaaac ttgctatcct tatcgtaaaa tacaaatgga agttcttctg
     4441 gtttgatgcc taattcaggg ttattggatc ttgagaaaac ttccatatcc gtagaaggat
     4501 ctttcccaac aatatgcaaa tatgtcttgc tgtccttttc acggtttgca tcgtggacat
     4561 catccgactg taagcggttg taaaggaaac tgttattatc tgccagccag gaaactggga
     4621 acccgccaaa acgacgatca atcttctctg gatatagata aagggtttct gtttctattg
     4681 tcagcaaaac tgcgctctca gatccgtttg gagcgatatc aatagcaact ttcgatccat
     4741 caatagaagg attaactgaa ctgatgacat atttctttgt agtgtcctgg ctataggttt
     4801 tgggatcgaa cattagtatt tctttcccct caaatccatc gcggtaataa agttttccag
     4861 tctcatcagc aggagtcatc tttaggtaaa aatagcgatc attatctatg atccttaaag
     4921 aacttattgt tgatgatttt cgtttatcaa agtcaaccat catattaatc attctttgtc
     4981 ttccgggaat actgttcaga attttgcgtg aataatctga ttgttgcttc atccattgcc
     5041 taaccgtagt atcctgtaga ttttccatat atctgtatgg atcggtgatc ttaacaccga
     5101 agtattcgtc cgtcacaggc tttaccggcg caaggggtgg ttttaatctt ttctcatttg
     5161 tagaacattg taccaataag aaaaatgata gaaatacaat aaatgtcttc gttttcatga
     5221 tttatgttct ttagatttta aaatattcca tttaatttcc aggtgttttt cttgatcact
     5281 caatcaaatt tatgaaattc agttggattg attgaggaaa gtcagagaaa atagtatgta
     5341 taaaatgtga ttattcactt cctttttctt ttagggaagc ttaacaagtc acaggcctcg
     5401 ttatccgatt aattattgca gggaagatct aaaaactgtc ccttccgata gctaccagaa
     5461 ggggctgagg tggaagaccg gagatttact tgcatgatcc cttaatcctc atccgtacaa
     5521 gcacggttcg ggttgtcaat aaaaaacccg gtgctgatgc gacagggatt tttcattgaa
     5581 gacaagaagt caactgaaat atcaaaccat gagttaatac gggatatgta tgtaaaagtt
     5641 cttaagtttg ggaaagtgat aatgggttca gatatggcat aagatttgtt ttttattgat
     5701 gaaaatgtga tatttgtctc tttttctttc aaaatgcatt ttattagttt gaatattctt
     5761 attaacttag taagtgttat taacaataat ctgcgctgaa ggaaagtaat taaagaacat
     5821 ttcgttactt tttacgttta attgttttgc tattttacaa tgcagttatg aataagaaaa
     5881 atatgtacca aaccgatttc ttgacagcaa actcctttgt cattgggata ggcagtgtat
     5941 ttaatctggc aggtaactat tttgaataca attattctcg taca
//
LOCUS       DAOSPX010000202         5932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12268_length_5932_cov_3.851285, whole genome shotgun sequence.
ACCESSION   DAOSPX010000202 DAOSPX010000000
VERSION     DAOSPX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12268_length_5932_cov_3.851285"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLZ75_09755"
     CDS             complement(<1..732)
                     /locus_tag="PLZ75_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOS72715.1"
                     /translation="MYTLIDGKKTAADIRREIAAEVADIRKSGGRVPHLAAILVGNNP
                     SSETYVANKVKDCEEVGFGSTLIRFDEKTGEKALIEKIRELNEDDSVDGFIVQLPLPA
                     HISEERVIEAIDPEKDADGFHPVNIGRMVIGAPGFLPATPYGIVELIKRYNITTSGSN
                     CVVIGRSNIVGRPVSILLSRKGMDATVTVVHSRTRNIGEIVKSADIIIAAIGSPDFVK
                     ADMVKEGAVVIDVGTTRIAAPDTKSG"
     gene            complement(746..2065)
                     /gene="ffh"
                     /locus_tag="PLZ75_09760"
     CDS             complement(746..2065)
                     /gene="ffh"
                     /locus_tag="PLZ75_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOS72716.1"
                     /translation="MFENLSERLDKSFKILKGQGRISEINVAETLKDVRRALLDADVN
                     FRTAKQFTDSVKAKALGQEVLRAVNPSQMMVKIVHDELTLLMGGEKTDINIRINPSVI
                     LIAGLQGSGKTTFSGKLARWVKTKRSKNPLLVAGDVYRPAAIEQLKVIGSQTEVPVYS
                     EEGNMNPVNIARNAIKEAKRNGNDVVIIDTAGRLAIDEEMMNEISAIRDAVTPHEILF
                     VVDAMTGQDAVNTAREFNDRLDFDGVVLTKLDGDTRGGAALSIRSVVNKPVKFVSSGE
                     KMDAIDIFYPERMADRILGMGDIVSFVEKAQEQFDAEEARKLQKKIARDQFDFNDFIN
                     QIQQIKKMGNVKDLMAMIPGVGKAVKDLDIDDNAFRSIEAIIRSMTPEERQNPQVLNG
                     SRRKRIALGSGTTVQDVNRLVKQFDETRRMMKMVSKGGNPAGLAGRK"
     gene            complement(2094..3887)
                     /gene="argS"
                     /locus_tag="PLZ75_09765"
     CDS             complement(2094..3887)
                     /gene="argS"
                     /locus_tag="PLZ75_09765"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOS72717.1"
                     /translation="MQMEIILKSRITEGLRILYNSEVSDDLVQIHETRKEFIGDFTLV
                     VFPLLRFSKNTPEKTGETIGRYLMDTFPSVTGFNVIKGFLNLEISPSWWISCFGGMLA
                     GDDLLGKCRSDDPETILIEYSSPNTNKPLHLGHIRNNLLGFSLYRILSACGHRVIKTN
                     IVNDRGIHICKSMLAWQKYGKGETPAKSGIKGDHLVGKYYVLFEREYRKQADVLIADG
                     MDPVEAKNNAPLLKEAREMLVKWEAGDPETMELWNMMNSWVYEGFNETYRKLGVDFDK
                     IYYESDTYKTGRDIIYNALQEGLLHENGDSSIWADLTDEGLDQKLLLRSDGTAVYMTQ
                     DIGTAVLRHEEFGFDRCIYVVGNEQNYHFQVLKAILKKMNYTWSEGLYHFSYGMVELP
                     EGRMKSREGVIVDADDLIAEMIETAREVSLELGKLSDFPGNEKGEIYRKIGLAALKYF
                     ILKVDPKKNMTFNPAESIDFNGNTGPFIQYTYARIRSVFRKAEEAGITFSRNISLTVG
                     AGAKEIQLIKLLRRFAFTVTEAAKTYSPALIANYCYDLAREYNQFYHDFTILGETDQT
                     VRDLRMGLSEITGKVLSTGMLLLGIEMPERM"
     gene            4127..4576
                     /locus_tag="PLZ75_09770"
     CDS             4127..4576
                     /locus_tag="PLZ75_09770"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HOS72718.1"
                     /translation="MKNVYIESTAKTPHVDFNPLTGELIFSGRSIPENPAKIYESLLN
                     WVQQYAKTPRKTTNLRLNIEYFNTASVIWIAKIVKVLCGMNESENTLLIHLYFDIEEF
                     DTMEADDIRDALSPVLDMIGTPSISLGIKIYGTDEKGEIIKESMVLV"
     gene            4655..>5932
                     /gene="amrB"
                     /locus_tag="PLZ75_09775"
     CDS             4655..>5932
                     /gene="amrB"
                     /locus_tag="PLZ75_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOS72719.1"
                     /translation="MRNTLALLIIMIFAMDLNSQSKLIDRQPVAAGRFYPAGKESLNR
                     DVSDLFAKCKKTPGNQKVRALITPHAGYVFSGRIAASALSTIPEDHSYDNIFIIGTSH
                     VMAFDGASAYYAGDYVTPFGKAIVNRDIANRLRKEHRVFNFPLTAHDQEHSIEVQIPL
                     IQYYFSTQPKIVPIIIGSRNNNTLRQIANALRPWFTPDNLFIISSDFSHYPSYRDANI
                     IDDLTAEGLISGDPGIFLETLRKNSAKNIRGLSTSMCGHTSGLVLLYLAEADKNLEFR
                     RIDYCNSGDSPYGDKDGVVGYHAIALVEKGNNPGTSARTESDFSFTEGEIRELFSIAR
                     NNIKSVLAEGKKASLPNPSGATAALKQPLGAFVTIRIDGKLRGCIGRFTSSDPLYEVV
                     RSSAVSSAFEDPRFSPLTSEEFEKCDLEITVLGP"
BASE COUNT         1521 a         1524 c         1199 g         1688 t
ORIGIN      
        1 tcctgatttt gtatccggag cagctatcct cgtggttccc acatctataa ccacagcgcc
       61 ctcctttacc atatcagctt ttacaaaatc cggtgaaccg attgcagcta tgatgatgtc
      121 ggccgacttt actatttcgc cgatattacg cgtacggctg tgaacaaccg ttactgttgc
      181 atccattccc ttgcgggaaa gcagtatgct taccggtctg ccgacaatgt tgcttcgccc
      241 tatcacaaca cagttgctgc ctgatgtagt gatgttgtaa cgcttaataa gctcaactat
      301 gccatagggt gtcgcaggaa gaaacccggg agcccctatc accatccgtc ctatattcac
      361 cggatgaaat ccgtcggcat ccttttccgg atctatggcc tcaatcaccc gttcttcgga
      421 tatatgtgcc ggaagaggaa gctggacgat aaatccgtcc acactgtcat cttcgttgag
      481 ctccctgatc ttttcaatca gtgccttttc cccggttttt tcatcaaaac ggataagtgt
      541 tgacccgaat cccacttctt cacaatcttt taccttgttg gccacatagg tttcacttga
      601 aggattgttt ccgacaagta tggctgccag gtgcggaacc ctgccgccgc tctttctgat
      661 atccgcaact tcagcggcta tttcccgcct gatatcagct gctgtctttt taccgtcgat
      721 aagtgtatac atagtgttgt ctgtgttatt tccttcctgc aagaccggcc ggatttccgc
      781 ccttcgacac catcttcatc atcctccgtg tttcgtcaaa ttgtttcaca agcctgttta
      841 catcctgaac ggtggtgccg cttccaaggg ctattctctt cctcctgctg ccattgagaa
      901 cctgcgggtt ttgccgttcc tcgggagtca tgctccgtat gatggcctca atacttctga
      961 aggcgttgtc atcaatatcc agatccttta ccgccttccc gactcccgga atcatggcca
     1021 taagatcctt tacatttccc attttcttga tctgctggat ctgatttata aaatcattga
     1081 aatcaaactg atcccttgca atcttcttct gcagcttcct ggcttcctca gcgtcaaact
     1141 gttcctgggc cttttcaaca aaggatacaa tatcacccat ccccagtatc ctgtcggcca
     1201 tacgttcggg gtagaagata tcgattgcat ccattttttc acccgaactg acaaacttga
     1261 ccggcttgtt aaccacggaa cggattgaca gggctgcacc tccgcgcgtg tcaccgtcca
     1321 gtttcgtaag aacaacgccg tcaaaatcaa gtctgtcatt gaattccctt gcagtattca
     1381 ccgcatcctg acccgtcatt gcatcaacta caaaaaggat ctcatgagga gtgacggcat
     1441 cccgtatcgc cgatatctca ttcatcattt cctcatctat ggccaggcgg cctgccgtgt
     1501 caatgatcac cacatcattc ccgttccttt ttgcttcttt aatggcattc cttgctatat
     1561 tcacgggatt catgtttcct tcctcggaat aaaccggaac ctctgtctgc gacccgataa
     1621 ccttcagctg ttcaatggcg gcaggcctgt aaacgtcacc tgccacaagc aaggggttct
     1681 tgctccgttt agtctttacc caccttgcaa gcttcccgct gaaggttgtt tttcccgatc
     1741 cctgcaatcc ggcaataagg atgacggaag gattgatcct gatatttatg tccgtcttct
     1801 cgcctcccat caggagcgtc agttcatcat ggacaatctt taccatcatc tgtgagggat
     1861 tcactgccct cagaacctcc tgccccagag ccttggcctt tacgctatcg gtaaattgct
     1921 tggcagtcct gaaattgaca tcagcatcga gaagtgccct gcgaacatcc ttcagcgttt
     1981 ccgcaacatt gatctcggaa attcttccct gccccttcag tatcttgaag gacttgtcaa
     2041 gccgttcact aagattctca aacatattaa ataagcattc tgttttttct ttttcacatc
     2101 ctctcaggca tttcaattcc caggagcagc atgcctgtgc tcaacacctt cccggttatt
     2161 tccgacagac ccatccggag atcacgtacc gtctggtcgg tctccccaag tatagtaaaa
     2221 tcatgataga actgattgta ttccctggcc aggtcatagc aatagttggc aataagggca
     2281 gggctgtagg tttttgctgc ttcggtaacg gtgaaggcaa accttctgag cagcttaata
     2341 agctgtatct cctttgctcc tgcaccgacc gtcaggctga tattcctgct gaaggtgatt
     2401 ccggcctctt ccgcctttct gaaaaccgat ctgatccttg cgtatgtgta ttgtataaaa
     2461 ggtcctgtat tgccgttgaa atcgatagat tcagccggat tgaatgtcat gttctttttc
     2521 ggatcaactt tcaggataaa atatttcagg gcagcaagac cgattttcct gtaaatttcc
     2581 cccttttcat tcccgggaaa atcagacaat ttgccaagtt cgagggatac ttcccgtgca
     2641 gtctcaatca tttcagcaat aagatcatcc gcatcaacaa tcaccccctc ccttgacttc
     2701 attctgccct cgggcagttc aaccattccg tatgaaaaat ggtacaggcc ttcggaccag
     2761 gtatagttca tctttttcaa aatggcctta agaacctgaa aatgataatt ctgttcattt
     2821 ccaaccacat aaatgcatct gtcgaagccg aattcttcat gcctcagcac agcagtccct
     2881 atgtcctgtg tcatgtaaac agcggtgccg tcggatctca gaagcagttt ctgatcaagg
     2941 ccctcgtcag tcaggtcggc ccagattgaa ctatcgccat tttcatgcag aagtccttcc
     3001 tgcagtgcat tatagattat atcccgaccg gtcttgtagg tatcggattc ataataaatc
     3061 ttatcaaaat ccacacccag cttcctgtag gtttcgttga atccttcgta tacccatgaa
     3121 ttcatcatgt tccacaactc catcgtttcc ggatctccgg cctcccattt cacaagcatc
     3181 tcccttgctt ccttcagaag cggtgcattg ttcttggctt ccacaggatc catgccgtca
     3241 gcaatgagaa catctgcctg tttcctgtac tccctctcga aaagaacata atatttacct
     3301 accaggtgat caccttttat gcctgatttg gcgggggtct ccccttttcc gtatttctgc
     3361 caggccagca tcgatttaca gatatgtatg ccgcggtcat tgacgatatt tgtctttatc
     3421 accctgtgtc cgcaggcact gagaatcctg taaagtgaaa acccgagaag attattccgt
     3481 atatgtccca gatgcagtgg tttgtttgta ttgggcgatg aatactcgat caggatggtt
     3541 tccggatcat ctgacctgca ttttcccaga agatcatccc cggcgagcat gccgccgaaa
     3601 cagctgatcc accatgacgg ggatatttca agattcagga agccctttat cacattgaaa
     3661 cccgtgacgg aaggaaaagt atccatcagg tatctgccga tagtctcacc tgttttttcc
     3721 ggagtattct tggaaaagcg caaaagagga aatacaacca gcgtgaagtc accaatgaat
     3781 tcctttcttg tttcatgaat ctgtacaaga tcatcgctca cctcactgtt gtagagaatt
     3841 cttaagccct ctgttattct tgactttaat atgatctcca tctgaatttc cccgtttcat
     3901 tttcagctga caaagatagt attaaaaaag aatgagaata tacaaatacg gaccaatttc
     3961 aggcaacact tctgtccggc ttacatcaaa agttgataat atttgataac tcctgccatt
     4021 tttttgataa agtttaagcg cttcctatgg aaataatata aaagatatac tatttttggt
     4081 aaggtttaaa tcacaataat atttatatca gcattgtcag gtggaattga agaatgttta
     4141 catagaatca actgccaaga caccacatgt tgactttaac cctctgaccg gagaactgat
     4201 tttttcaggg agatccatac cggaaaatcc tgcaaagatt tatgaaagtc ttctcaactg
     4261 ggttcagcaa tatgccaaga ctcccagaaa aacaaccaac ctgcggctga atatcgagta
     4321 tttcaatacc gcatcagtta tctggattgc caagatagtc aaggtcctct gcggaatgaa
     4381 tgaatcggaa aataccctgc tgatacatct ctattttgac attgaggaat ttgacaccat
     4441 ggaggcagac gacatcaggg atgccctgag tcccgtcctt gatatgatag gcactccctc
     4501 aatcagcctg ggcataaaaa tttacggcac agacgaaaag ggggaaatca tcaaggaatc
     4561 aatggtcctc gtataggacc cggcaacaaa cctgttcaga tatctttttt tcaattattt
     4621 ggcctaactt tattacgcca aattaattac agctatgcgt aacacattag cactcctcat
     4681 cattatgata tttgccatgg atctcaattc tcaaagtaaa cttattgacc gacaacctgt
     4741 tgctgcagga agattctatc ccgccggaaa agaatcactc aacagggatg tttccgatct
     4801 ttttgccaaa tgcaaaaaga cgcccggaaa ccagaaggtc agggcattga tcacccccca
     4861 tgccggttat gttttttccg gcaggattgc cgcatcggct ctttccacca ttccggaaga
     4921 tcacagctat gacaatatat ttatcatcgg aaccagtcat gtaatggcat ttgacggcgc
     4981 ttcagcatat tatgcgggcg attatgtcac gccgttcggt aaagccatag tgaacaggga
     5041 tattgcaaac aggctgagaa aagaacacag ggttttcaat ttccctctta ccgcacatga
     5101 tcaggaacac agtattgagg tgcagatacc cctgatacag tattacttca gcactcagcc
     5161 caaaattgtg ccgataataa tcggaagcag gaacaacaat acgctaaggc agattgccaa
     5221 tgccctgaga ccctggttta caccggataa cctgtttata atcagcagcg atttttcaca
     5281 ttacccttcc tacagggatg ccaatatcat cgatgatctc acggctgaag gactgatatc
     5341 gggcgatccc gggatttttc ttgaaacact gaggaagaat tctgcaaaaa acataagggg
     5401 attatccaca agtatgtgcg gccatacatc aggacttgta cttctgtatc ttgcagaggc
     5461 ggataagaat cttgagttca gaagaattga ttactgcaat tcgggtgatt caccatatgg
     5521 cgacaaggac ggggttgtgg gctatcatgc aatagcactt gttgaaaaag gcaataaccc
     5581 cggaacttcg gcaagaacgg aaagtgattt ctcttttacc gaaggtgaaa tccgtgagct
     5641 tttcagtatt gccagaaaca atattaaaag tgtactggca gaagggaaaa aggcctcact
     5701 gccgaatccg tccggagcta ccgccgccct gaagcagcca ctgggtgctt tcgtaaccat
     5761 caggatcgac ggaaagctaa gaggctgcat aggacgtttc acctcatcgg atcctcttta
     5821 tgaagtggtc aggtcctctg ccgtttcctc ggcatttgaa gatccgaggt tcagccccct
     5881 cacatccgag gaatttgaaa aatgcgacct ggaaatcact gttcttggtc cc
//
LOCUS       DAOSPX010000203         5841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12517_length_5841_cov_4.215175, whole genome shotgun sequence.
ACCESSION   DAOSPX010000203 DAOSPX010000000
VERSION     DAOSPX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12517_length_5841_cov_4.215175"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLZ75_09780"
     CDS             <1..405
                     /locus_tag="PLZ75_09780"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS72720.1"
                     /translation="RQAVILGGRPQERVQKELMGHITCYSHAGRREMQEIISGSKLII
                     ARAGYTTIMELVSLNCTALLIPTPGQTEQEYLASRLSEKGWFSRISQKDLKNVLPPIP
                     ELPGMKNDIMKQSAVLLEKVLKEISDEAEIQQ"
     gene            complement(379..1014)
                     /locus_tag="PLZ75_09785"
     CDS             complement(379..1014)
                     /locus_tag="PLZ75_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72721.1"
                     /translation="MMLSEKSISFDDRLAQLVSVLFHPLLMPVYGLLIIFTCPALYGY
                     LPFQVKKIMLFIVIINNVLIPLMMITYFRFRNLISTWTIDDRRERILPLVITTFFYSF
                     TVYLIYRFHIPLFIKSFIICATALVLAVTIINFWFRISLHATGCGAITALVMVLSVRM
                     QIPLTWLLIIVILVSGLVMSSRLWLKAHTPPEIWTGFFLGSVGSLLYLGLV"
     gene            complement(1011..2468)
                     /gene="rpoN"
                     /locus_tag="PLZ75_09790"
     CDS             complement(1011..2468)
                     /gene="rpoN"
                     /locus_tag="PLZ75_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOS72722.1"
                     /translation="MLKQKLQQKLLQKLSPQQIQMIKLLEVPTLQIEQRIKKELEENP
                     ALEEGTDYEDIQTEQQDDRDYEDNDRDQEEFTLDDYIEEDDIPDYRLQTYNVSRDDEK
                     RAEIPFSGGSSFHEYLESQYSLRNQTEKQKILGEFIIGNIDEDGYLRRELENIVDDLA
                     FLQNISTTVEELEEVLEVIQDLEPVGVGARTLRECLLLQLAKKENTQPAEQLAYSILD
                     NYFEEFSKRHYDKIIARMGISEDELKQAIEEILKLNPKPGGFYSDPYSKHAQPIIPDF
                     ILEHSEEGFDLHLNSKNVPELRLNPAYSEMLQAYSRQKGDKKQMKDAIVFMKQKIDSA
                     RWFIDAIKQRQNTLLLTMNAIFEYQQEYFIDGDETRLRPMILKDVAEMTGLDISTVSR
                     VANSKYIQTHFGIFPLKYFFSEGLQTDSGEEVSTREIKRILQDCIDNEDKRRPLTDER
                     LTEILQEKGYQIARRTVAKYREQLNIPVARLRKEI"
     gene            complement(2502..3890)
                     /gene="asnS"
                     /locus_tag="PLZ75_09795"
     CDS             complement(2502..3890)
                     /gene="asnS"
                     /locus_tag="PLZ75_09795"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOS72723.1"
                     /translation="MKREKIKALLASGGTDREVLVKGWVRTKRGSKNIAFIALNDGST
                     IKNIQVVADVSGFDEQLMKEITTGACIAVRGRLAGSEGSGQDVEIRAESIELYGKADP
                     ETYPLQKKGHTMEFLREIAHLRPRTNTFGAVLRIRHALAYAIHKYFNDKGFYYLHTPI
                     ITSSDAEGAGEMFQVTTLDLGSVPKKTDGSVDFSDDFFGKPTNLTVSGQLEGELGALA
                     IGEIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDLNDNMDLAEDFVKYLIRYALEN
                     CSDDLDFLNKMISKDLLERLDFVINNEFIRMTYTEAVGILEASGRKWVYGTGWGKDLQ
                     AEHERYLVEEYFRCPVILTDYPKVIKAFYMKQNDDGKTVRAMDVLFPGIGEIIGGSQR
                     EENYEKLLARIQELGLPEKDLWWYLETRKFGTAPHSGFGLGFERFVQFVTGMANIRDV
                     IPFPRTPKNAEF"
     gene            4004..4855
                     /locus_tag="PLZ75_09800"
     CDS             4004..4855
                     /locus_tag="PLZ75_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOS72724.1"
                     /translation="MDENQKVIVVPWDFTPVAENALAHAAKIGRMVKNGIALLHIVDT
                     GIKPREEGEKKTLLQHAASEASRKYNMPVSSYVVRGTIFKSIAEFVNEKEADVVVMGT
                     HGMKGMQKLTGSWALKVIAKSKVPFIVVQDPPRDQERYQNILFPVDFRRETKEKIKMA
                     IYMGKYFDSKMHIMVTSTYDKSLQKRTKVNLAFAIKYFIQNNIEYEVHEMPRGKTAEQ
                     TLEFAQKINADLILIITTKNISFADYVLGASEQYIIANSSRIPVCCVNPKASFASSRQ
                     FMGGWGD"
     gene            4902..5816
                     /locus_tag="PLZ75_09805"
     CDS             4902..5816
                     /locus_tag="PLZ75_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS72725.1"
                     /translation="MSIVVRGVSKVYGSQKALDNVSFEVKTGEIVAFLGPNGAGKSTM
                     MKIITGFIPATSGTVLVNGTDVSSGGREIRKITGYLPENNPLYPEMYVREYLSFVASM
                     YGSRRKQGSAVNDIIGLTGLAPEANKKIGALSKGYRQRVGLAQALIHDPDVLILDEAT
                     TGLDPNQIIEIRNLIREAGKEKTVMLSTHIMQEAEAICGRIIIIDRGVIVADEEKDKI
                     HQKIKSPKQLIRAGFDRETSDSELLGIPGIKSAVLTRQGEWLLEAESGEDIRPAIFAF
                     AVRNNLTVLSLQKEESNLEEIFRQLTGR"
BASE COUNT         1530 a         1392 c         1374 g         1545 t
ORIGIN      
        1 aggcaggccg ttatcctcgg cggcaggcca caggaaagag tgcagaagga actcatgggg
       61 catatcacct gttacagtca tgccgggcgc cgggaaatgc aggaaataat ttcaggcagc
      121 aagctgatca ttgcaagggc aggctacacc accatcatgg aacttgtcag ccttaactgc
      181 actgccctgc tcatacccac acccgggcag accgaacagg aataccttgc atcgaggctt
      241 tcggaaaagg gatggttcag ccgtatatcc cagaaggatc tgaaaaacgt acttcccccg
      301 ataccggaac ttcccggcat gaagaatgat ataatgaagc aaagtgccgt acttctggaa
      361 aaagtcctga aggaaatatc agacgaggcc gagatacagc agtgatccca ccgaacccag
      421 gaaaaatccc gtccatattt ccggcggagt atgagccttg agccacaaac gcgaagacat
      481 caccagtcct gataccagta ttacaatgat cagaagccat gtaagaggga tctgcatcct
      541 caccgagagc accatgacca gggctgttat tgcaccgcaa cccgtggcat gaagggatat
      601 tctgaaccag aagtttatga tggtcacggc aaggaccagt gcagtggcac agatgatgaa
      661 tgatttgata aacaggggta tgtggaacct gtaaatcagg tatacggtaa atgagtaaaa
      721 gaatgtggtg atgacgagcg gcagtatcct ttcccttctg tcgtcgattg tccaggtgct
      781 tatcaggttt ctgaacctga aatatgtgat catcatgagg ggaatcagga cattgttaat
      841 gatcacgata aacagcatta tttttttaac ctgaaaggga aggtatccgt aaagggccgg
      901 acaggtgaat atgatcagca ggccgtaaac cggcatcagc agggggtgaa acaaaactga
      961 tacaagctgt gcaagcctgt cgtcgaatga tattgatttc tcgcttaaca tcatatttcc
     1021 tttctgagcc gtgcaacggg aatattcagc tgttcacggt attttgccac ggttcttctg
     1081 gctatctggt agcccttttc ctggagtatc tctgtcaggc gctcatcggt cagcggccgg
     1141 cgcttgtcct cattgtcgat gcaatcctga agaatccgtt ttatttccct ggtcgacacc
     1201 tcttcgccgc tgtcggtctg aagtccttca gagaaaaaat acttcagcgg gaagatgccg
     1261 aaatgcgtct gtatgtattt gctgtttgcg actctggaaa ccgtggatat gtcaagtccg
     1321 gtcatctcgg caacatcctt caggatcatc ggtctgagcc tggtttcatc cccgtcgata
     1381 aaatattcct gctggtattc aaaaatggcg ttcattgtaa gcaggagagt gttctgacgt
     1441 tgcttgatgg catcaatgaa ccatcttgcc gagtcaattt tctgcttcat gaatactatt
     1501 gcatccttca tctgcttctt gtcacccttc tgcctgctgt aagcctgcag catttcggaa
     1561 tatgctggat tgagcctcag ttccggaacg ttcttcgaat tgagatgaag atcaaatcct
     1621 tcctcggaat gttccagtat gaaatcgggg attataggct gggcatgttt gctgtaggga
     1681 tcactgtaga acccgccggg cttgggatta agtttcagga tttcctctat tgcctgcttc
     1741 agttcatcct cgctgatacc cattctggca attatcttgt cataatgtct ttttgagaac
     1801 tcttcaaaat agttatccaa tatcgaatac gccagttgtt ccgcaggctg tgtgttttcc
     1861 tttttcgcaa gctgcaggag aaggcattcc ctgagtgttc tggctcccac accaacaggt
     1921 tcaagatcct gaatcacttc cagaacctct tcaagttcct caaccgtggt gcttatgttc
     1981 tgcaggaagg caagatcgtc aacgatgttt tcaagttccc ttctgagata tccgtcctcg
     2041 tcaatgttac cgataatgaa ttcccccagt atcttctgct tttccgtctg gtttctcagg
     2101 ctgtactgtg attcgaggta ttcatgaaac gatgaaccgc ctgaaaaggg gatctctgcc
     2161 cttttctcat catccctgct tacattgtat gtctgaagcc tgtagtcggg aatgtcatct
     2221 tcctcaatat aatcatcaag cgtaaattcc tcctgatccc tgtcattatc ctcatagtcc
     2281 cggtcgtcct gctgttctgt ctgaatatcc tcataatcgg taccttcctc aagagccgga
     2341 ttctcttcaa gttccttctt gatccgctgt tctatctgca gggtgggcac ctccagcagt
     2401 ttgatcatct gtatctgctg gggagacagt ttctgaagta gcttctgttg taacttctgc
     2461 tttaacattt ccgccggttc ggtcagttta ctcctaatat atcaaaattc tgcgtttttc
     2521 ggtgttctgg gaaagggtat aacatcccgg atattcgcca ttccggttac aaactgtaca
     2581 aatctctcaa aaccaaggcc aaagccgcta tgaggcgcgg ttccgaattt cctggtttcc
     2641 aggtaccacc agagatcctt ctccggcaga ccgagttcct gtattcttgc aagcagtttt
     2701 tcgtaatttt cctccctctg tgaacctccg attatttccc ctattccagg gaaaagcacg
     2761 tccatggccc tgacggtttt gccgtcatca ttctgcttca tataaaaagc ctttatgacc
     2821 ttcggataat cggtaaggat taccgggcat ctgaaatatt cctcaacaag ataacgttca
     2881 tgctctgcct gaaggtcctt gccccatcct gttccgtata cccatttcct tcccgaagct
     2941 tcaagaatgc cgacggcttc ggtataagtc attctgataa attcattatt gatgacaaaa
     3001 tcaagcctct ccagaaggtc cttgctgatc atcttattga ggaaatcgag gtcgtcactg
     3061 cagttctcaa gggcatacct tatcaggtac ttgacaaaat cctccgcaag gtccatattg
     3121 tcgttaaggt catagaaggc catctccggt tctatcatcc agaactccgc gagatgcctg
     3181 ggggtattgg agttctcggc acggaacgtg ggtccgaagg tatatatttc accgatggca
     3241 agggctccga gctcgccttc aagctgtcct gaaacagtaa gattcgttgg ctttccgaag
     3301 aagtcgtcac tgaaatccac cgatccgtct gttttcttcg gcacactccc caggtcaaga
     3361 gtggtaacct ggaacatttc tcccgcacct tcggcatcac tggaggtgat tatgggagta
     3421 tgaagataat agaagccctt gtcgttgaaa tacttgtgaa tagcataagc cagtgcgtgt
     3481 cttattctga gaaccgcacc aaaggtattc gtgcggggac gcagatgtgc tatttccctc
     3541 aggaattcca ttgtatggcc tttcttctga agagggtagg tctcagggtc ggcttttccg
     3601 taaagctcaa tcgattcagc gcggatctcc acatcctggc cgcttccttc cgatccggca
     3661 agcctgcctc tgacagcaat gcatgcaccg gtggttattt ccttcataag ctgttcatca
     3721 aaaccggaca catcggcaac cacctgtata ttctttatcg ttgatccgtc attgagagcg
     3781 ataaatgcaa tattcttgct tcctctcttt gttctgaccc atcccttgac gagtacttcc
     3841 ctgtctgtcc ctcctgaagc cagaagggct tttatttttt cccgtttcat cttaaaaatt
     3901 aaacatcccg caaaaataat aaaaacaaaa cactgctgcc cgccggtttg aatatttcta
     3961 aaaacttaat aactttgaat tcaaactgta acaaaacatt gctatggatg aaaatcagaa
     4021 agttattgtc gtgccctggg atttcacccc agttgccgaa aatgctcttg cacatgcggc
     4081 aaagataggc cggatggtaa agaacggcat tgccctgctg catattgttg atacgggaat
     4141 caagcccagg gaagagggag aaaagaaaac cctgcttcag catgctgcca gcgaagcttc
     4201 ccggaaatac aatatgcccg tttcgtctta tgtggtcaga ggaacaattt tcaagtcaat
     4261 tgcggaattt gtgaacgaaa aggaggcaga tgttgttgta atgggcaccc atggcatgaa
     4321 gggcatgcag aagcttacag gaagctgggc cctgaaagtg atagccaaat cgaaagttcc
     4381 cttcatagtt gttcaggatc ctccccgtga tcaggaaagg tatcagaaca tcctgtttcc
     4441 cgttgacttc cggagagaga ccaaggaaaa gatcaaaatg gccatctaca tgggaaaata
     4501 ttttgattca aagatgcata taatggtcac ctcaacatac gataagtctc ttcagaaaag
     4561 gaccaaggtc aacctggcct ttgccatcaa atacttcata cagaacaata tcgaatatga
     4621 ggttcatgag atgcccaggg gcaagactgc ggagcagacc ctggaatttg cccagaaaat
     4681 caatgcagac ctgatactga tcataaccac gaagaatatc tcctttgcag attatgtgct
     4741 gggagcgtca gaacagtaca ttattgcgaa cagttcccgg attccggtct gctgtgtcaa
     4801 tccgaaagca tcctttgcaa gcagcaggca gttcatggga ggctggggcg attagcacaa
     4861 gatcccgaat ctgttgtctg ctgtcggaaa atgaaagata aatgtccatt gtagtcaggg
     4921 gggtcagtaa ggtttacgga tctcagaaag cgcttgataa tgtttccttt gaagtgaaaa
     4981 cgggggaaat agtggcattc ctcggaccca acggtgcggg aaagtccaca atgatgaaga
     5041 tcattaccgg cttcataccg gcaacttccg gaacggtgct ggtcaacggc acggatgtct
     5101 cgtccggcgg acgcgaaata aggaagataa cgggttatct cccggaaaac aatcccctct
     5161 atcccgaaat gtatgtcagg gaatacctgt catttgtcgc ctcgatgtat ggttcccgca
     5221 gaaagcaggg gtcagcagtg aatgatatta tcgggcttac ggggctggcg cccgaggcaa
     5281 acaaaaagat cggcgccctt tcaaagggct acaggcagcg tgtgggactg gcccaggcgc
     5341 tgattcacga tccggatgta ctcatactgg atgaagccac aacggggctc gatccgaatc
     5401 agatcattga aatacggaat ctgatcaggg aagccggaaa ggaaaagaca gtcatgcttt
     5461 caacccatat catgcaggaa gccgaggcaa tctgcggaag gataattatt atcgacaggg
     5521 gagttattgt tgccgatgag gaaaaagaca agatacacca gaagatcaag agcccgaagc
     5581 agcttatcag ggccggtttt gacagggaaa caagtgattc ggaactgctt ggcattccgg
     5641 ggattaaaag tgcagtcctg acaaggcagg gtgaatggct cctggaagca gaatcagggg
     5701 aagatatccg cccggccata tttgcatttg ccgtcaggaa taacctgact gtgctttccc
     5761 ttcagaaaga ggaaagcaat cttgaagaaa ttttcaggca actgacaggc agataatttt
     5821 tttattccgc ttacttttct t
//
LOCUS       DAOSPX010000204         5784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12654_length_5784_cov_4.616862, whole genome shotgun sequence.
ACCESSION   DAOSPX010000204 DAOSPX010000000
VERSION     DAOSPX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12654_length_5784_cov_4.616862"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PLZ75_09810"
     CDS             <1..915
                     /locus_tag="PLZ75_09810"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOS72726.1"
                     /translation="YNVEPFTMTDTTNIPDRYKTIAIVRPSDTISSNHFGQLDRFLAR
                     GGNIFLALNRVEGDFSTATGHSVSTGLEQWLLQKGLTVADNFVIDASCGAVTVQQQQG
                     NFIMTTQVSFPYFPIISNFADNSITRGLESVVLQFASPIIFSGDSTKKFTPLAFSSER
                     SGTLSAPLYFDIERQWTENDFPMSDIAVAGILEGKLSGDLNSRIVLVSDGDFAVSGQQ
                     RGQQLPADNISLMVNSIDWLSDDTGLIDLRTKEVTSRPIKEISDGSRSFLKWFNFLLP
                     IVLIIVYELIRMQINRNRRIKRMEVSYE"
     gene            908..1870
                     /locus_tag="PLZ75_09815"
     CDS             908..1870
                     /locus_tag="PLZ75_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOS72727.1"
                     /translation="MSRRFNNRKLLVVLGGLLILLLLTLVIKIPRERSTLKGTLVAFD
                     TAGVELITIVPKAGLSEEFRFTRNNGRWNIIQGETVAVPVQGAVENILSGIMAIKPQT
                     LAAVGKSAQEEYELTDSLATRIKVTGKRGRKLAEVLIGKFSYRQSASPYAGYGRNNFD
                     VTTYVRLAGEEEIYAVDGFLALTFSGGFNDWRDKTFIRCKRDDINRITFTYPADSSFS
                     LVKRDSFWFAGNQKADSLKVADYLSYLSLAEGREFADTFRPGSYPSYQILIEGNNLLS
                     ITVKCFRDGDDQFILNSSLNPGVYYSSDRDGMFAELFKPRNHFF"
     gene            2025..3365
                     /locus_tag="PLZ75_09820"
     CDS             2025..3365
                     /locus_tag="PLZ75_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOS72728.1"
                     /translation="MKRTMILIFLLAFSIISDAQLRVGVAMKSITPETPFWLTGYASR
                     DRPSTEVLHDLWAKALVIEENSRSRIVIVTTDILGLSRDISEEVSRRVLAEHGMDRSQ
                     LILSSSHTHSGPVIWPALSVIFDFDREDQKTVMRYAEKLTDDIVEVVNMAFSAMEPMQ
                     LSYGRGNADFAINRRQATDKGVVIGLNPAGTVDHDVPVLKAADMNGNVKAVLFGYACH
                     NTTSASYLINGDYSGFAQIEVEKMFPGAVAMYMAGCGADQNPNPRGSVELAAEHGRSL
                     AEAVNTAMKGEMKPVRPPLRTSYIVTELHFRPFDPGIYRREMTGEDIYMQRRARLMLE
                     AYNKGWDVSTYRYPVQAVRFSNDFTILALTGEVVLDYSIAARARFPRENMFVAGYCTE
                     VQCYIPSKNILREGGYEPESSMIYYGFPGSFTEDVEDRVMDAVALVMKRSGAGR"
     gene            3441..3908
                     /locus_tag="PLZ75_09825"
     CDS             3441..3908
                     /locus_tag="PLZ75_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72729.1"
                     /translation="MKKLLIVLCLITVVAGQAAAQDYNTGIGFRGGPFNGVTVKHFIK
                     QNGAVEVLMASRWRGFEATGLYEIHYDIRNVDRLKFYFGAGGHLGVYNGKHTGWNDNE
                     GAYLVLGADLVLGIEYSFRELPINLGFDWKPAYNILGSRFVADGAAFSIRFIF"
     gene            4160..5257
                     /locus_tag="PLZ75_09830"
     CDS             4160..5257
                     /locus_tag="PLZ75_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS72730.1"
                     /translation="MGNLYRLSDAKSRSISPENFTGEKGRGGMADPADRDKPNVANSA
                     SAARDLGQGWKVNPFVRIKPGETFTLAEIDGPGAIQHIWMTPAGNWRFSILRIYWDNE
                     KEPSVECPVGDFFGMGWGSYAQLSSLAVCVNPGSAFNCYWTMPFRKKCRITMENTGDT
                     DQMTLYYQIDYTLTDVPADAAYFHAQFRRLNPNVTSDYTIVDGIRGKGHYVGVYMAWG
                     VNNNGWWGEGEIKFFMDGDSKFPTICGTGTEDYFCGSYDFDTRKKNPAGVEEVNYTEF
                     CTPYAGLHQVIRGDGHYNVMQRFGMYRWHILDPIRFEKDLRITIQDLGWRHGGRYLPQ
                     KSDIASVCYWYQTEPHAKFPAIPGWRELEVN"
     gene            5476..>5784
                     /locus_tag="PLZ75_09835"
     CDS             5476..>5784
                     /locus_tag="PLZ75_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72731.1"
                     /translation="MENDKQSKANIQKGTGRRKFIASAAMATIGTIGAAGIISSCSRK
                     PKEKLPVLLNEAPDGRVLKAGLIGCGGRGTGAAVNFLDAGPNLEITALGDIFQDKIDN
                     C"
BASE COUNT         1586 a         1216 c         1475 g         1507 t
ORIGIN      
        1 tacaatgttg agccttttac catgaccgat acgacaaaca ttccggacag gtataagacc
       61 attgccattg tccggccttc tgatacaata agttcaaatc atttcgggca gcttgaccgt
      121 tttctcgcca ggggaggcaa catattcctt gccctgaatc gtgtggaggg agatttttca
      181 acagcaacgg gtcattctgt ttccacaggg cttgaacaat ggctcctgca gaaaggtctg
      241 acggttgcgg acaacttcgt tattgatgca agttgcgggg ctgtgactgt tcagcagcag
      301 caaggcaatt ttataatgac cacgcaggtg tcattcccgt atttccccat aatatcaaat
      361 ttcgcggaca attcaattac caggggtctg gaatctgtcg tgctgcagtt tgcgagtccg
      421 atcattttca gtggtgacag tacaaaaaaa ttcactcccc tggcgttttc ctcggaaaga
      481 tcaggaaccc taagcgctcc tctgtatttt gatattgaaa ggcagtggac ggaaaatgat
      541 tttcccatgt ctgacattgc agtggccgga attcttgaag gaaagttgtc aggcgacctg
      601 aattccagaa tagtactcgt ttctgacggg gattttgcag taagcggcca acagcgcggc
      661 caacagctgc ctgcagacaa tatcagcctt atggtcaatt ccattgactg gctctcggat
      721 gataccggac tcattgatct caggacaaag gaagtgacat caagacccat aaaggagata
      781 tctgacggca gcagaagttt tctgaaatgg ttcaatttcc ttctgcccat agttctcata
      841 attgtatatg aactgataag aatgcagatc aaccgcaaca gacggattaa aagaatggag
      901 gtaagctatg agtagaagat ttaacaacag gaagttactg gtagtcctgg gaggattgct
      961 gatactgctt ttgcttacgc tggtaattaa aatcccacgt gaaagatcga ctctgaaggg
     1021 cactctggtt gcatttgaca cggccggcgt ggagcttatc actattgttc cgaaggccgg
     1081 attgtcggag gaattcaggt tcacgcgcaa taatggcagg tggaacataa tccagggcga
     1141 aacagttgcc gtcccggtgc agggtgctgt tgaaaatatc ctttcgggga taatggcaat
     1201 aaagccacag acactggccg ctgtgggtaa atcagcgcag gaggaatatg agcttactga
     1261 cagccttgca accagaataa aagttacagg caaaaggggc aggaagcttg ccgaagtgct
     1321 gatcggtaaa tttagctaca gacagtctgc cagtccctat gcaggctacg gaagaaacaa
     1381 cttcgatgtc accacctacg taagacttgc aggagaagag gagatttatg ctgttgatgg
     1441 ctttcttgcc cttaccttca gcggcggatt caacgactgg agggataaaa catttataag
     1501 gtgcaagcgg gatgacataa acagaataac attcacttat cctgccgaca gcagtttcag
     1561 tcttgtaaaa agggattcgt tctggtttgc cggcaatcag aaggcggatt cgcttaaggt
     1621 tgctgactat ctcagttacc tgagcctggc tgaaggaagg gaatttgccg atactttccg
     1681 gcccggttcc tatccgtcgt accagatact catcgagggg aacaacctgc tcagtataac
     1741 agtgaaatgc ttccgggacg gggatgatca gtttatcctt aattcaagcc ttaatcccgg
     1801 ggtatattac agcagtgaca gggacggcat gtttgcggaa ttattcaaac cgcggaatca
     1861 ctttttctga ttcaccgttt ttctgattca ctgtttttca tctttaataa cggcatttgc
     1921 gggccggttg cgaactctgt aatttgacgg attccgggag gcgcagggtg atatgcttaa
     1981 ataattttta gtaaattgtg tcctttaatt ttaatccgga aatcatgaaa aggacgatga
     2041 ttttaatttt ccttctggca ttcagtatta tttctgatgc ccagttgagg gttggtgttg
     2101 ccatgaaatc aattaccccg gaaacacctt tctggctgac aggctatgca agccgtgaca
     2161 ggccgtccac ggaagtatta cacgatctgt gggcaaaagc gctggtaata gaggaaaaca
     2221 gcaggagcag gatagtcatc gtaacaactg atattctcgg cttatcccgc gacatatcgg
     2281 aagaggtttc ccgaagggtt ctggctgaac atgggatgga ccgctcgcag cttattctca
     2341 gttcgtcgca tactcattcc ggaccggtaa tatggccggc actcagtgtc atcttcgatt
     2401 ttgaccggga agatcagaaa accgtgatga gatatgcaga gaagcttact gatgatattg
     2461 tggaagttgt gaatatggcg ttttcagcga tggaaccgat gcagctctcg tacggcaggg
     2521 gaaatgctga ctttgcaata aaccgccgtc aggccacgga caagggtgtg gtaatcggac
     2581 tgaatcctgc cgggactgtt gatcatgatg tgcccgttct gaaggcagct gatatgaatg
     2641 gcaacgtgaa ggcggttctt ttcggatatg catgtcacaa taccacttcg gcttcatatc
     2701 tgattaacgg cgattattcc ggttttgcac agattgaagt ggagaagatg ttcccgggag
     2761 ctgttgccat gtatatggcg ggctgtggcg cagatcagaa tcccaatccg agaggttcgg
     2821 tggagctggc tgctgaacat ggcagatccc ttgctgaagc agttaatact gccatgaaag
     2881 gagaaatgaa acctgtaagg cctcctcttc gtacaagcta tatcgtgacg gaactgcatt
     2941 tccggccttt tgaccccgga atataccgca gggaaatgac aggagaggat atctatatgc
     3001 aaagacgtgc caggcttatg ctggaggctt ataacaaggg atgggatgtt tccacgtacc
     3061 gctatcccgt tcaggcagtg aggttcagta atgatttcac cattcttgcg ctcaccgggg
     3121 aggttgtact ggactattcc attgccgcca gggccaggtt cccccgtgaa aacatgttcg
     3181 tggcagggta ttgcaccgag gtacaatgct acataccttc aaaaaatata cttcgcgaag
     3241 gaggatatga gcctgaaagc agtatgatat attatggatt tccggggtcg ttcacagaag
     3301 atgttgaaga cagggtaatg gatgctgttg ccctggtaat gaagcgcagc ggggcaggga
     3361 gatgataatg gccttctccg gcacacggaa cctgaaaaac tgtttttcag taatgacttg
     3421 ttgaaccaat attatcactg atgaaaaaat tgctgatcgt tttatgcctg attacggtag
     3481 tggcagggca agctgcagcc caggactaca ataccggtat cggattccgg ggcggaccct
     3541 tcaatggtgt gacagtcaaa cattttataa aacaaaatgg agctgttgaa gtgcttatgg
     3601 catcaaggtg gaggggcttt gaggcaaccg ggctgtatga aatccattac gatatcagga
     3661 atgttgacag gctgaagttt tatttcggag caggaggaca tcttggcgtt tacaacggta
     3721 aacatacagg ctggaatgat aatgagggcg cctatctggt tctgggtgct gatctggttc
     3781 tcggtattga atacagtttc agggagttgc ccataaacct tggatttgac tggaaaccgg
     3841 cttacaatat tctgggatca aggtttgtgg ctgacggagc tgccttttct ataagattta
     3901 ttttctgaca gatatttccc tgcaactgca tatcaggctg ttttctcctg atttcaacaa
     3961 ttctttaaat aaagcggagg attttcagat taattcaaaa aagcagttac aattcatctg
     4021 taattattaa ttttagattg atttaccaaa ctcaatctga aaatatacta tgagaacaaa
     4081 tctgacaatc ttaattcttt tatgcctgat tcccttaacc ggatcatccc agggcaagtt
     4141 caacggactt gatctgaata tgggaaatct ttacaggctt tcggatgcca aatcccgttc
     4201 aataagccct gaaaacttca ccggtgaaaa gggcagaggt ggtatggctg atcctgcaga
     4261 cagggataaa cccaatgttg ccaattcggc cagtgcagca cgtgatctcg ggcagggctg
     4321 gaaagttaat ccttttgtca ggattaaacc gggcgagact tttacccttg ccgaaattga
     4381 cggtccaggt gcaattcagc atatctggat gactcccgca ggcaactggc gcttttccat
     4441 tcttcgcatt tactgggaca atgagaagga accttcagtg gaatgccccg tcggtgactt
     4501 tttcggaatg ggctggggca gctatgcaca gctatcctcg ctggcggtat gcgtgaatcc
     4561 gggaagtgca ttcaactgct actggaccat gccattcagg aaaaaatgca ggataacaat
     4621 ggagaacaca ggggatactg atcagatgac actgtattat cagattgatt atactcttac
     4681 tgatgtcccg gcggatgcag cctatttcca tgctcagttc cgcaggctta atcccaatgt
     4741 gacttccgat tataccatcg ttgacggcat caggggaaaa ggccattatg ttggggtgta
     4801 tatggcctgg ggtgtcaaca acaacggctg gtggggagaa ggtgaaatca agtttttcat
     4861 ggatggggac agcaagtttc cgaccatatg cggaaccgga accgaagatt atttttgcgg
     4921 ctcatatgat tttgacaccc ggaaaaagaa tccggcaggc gttgaggagg ttaactatac
     4981 tgaattttgt acgccctatg ccggattaca ccaggtgatc cggggagacg gccattacaa
     5041 cgtgatgcag cgttttggca tgtaccgctg gcatatcctt gatcccatac gttttgagaa
     5101 agatctgagg ataaccatac aggatctggg atggcggcat ggcggaagat accttcccca
     5161 gaaatccgat atcgcctcgg tatgttactg gtatcagacc gagccgcatg caaaatttcc
     5221 tgcgataccg ggctggagag aacttgaggt aaactaaccg gggaaatatt atatgcatat
     5281 tgaaaatcaa tacataaaaa accatatatt taaaatcgga atttattgaa tcagaagacg
     5341 gggcgcaata cgcttcgttt aacggtggct acattgcaac atgttgggat tacaggacct
     5401 gttggaagca acaattacag aaccaataat cagagaatca tatattatag aatcaataat
     5461 tataaaacct aaaagatgga aaacgataaa caatcaaagg cgaatattca aaaagggaca
     5521 gggcggcgga aattcattgc cagtgcagca atggccacca tcggcactat tggtgcagcc
     5581 ggtattattt cctcatgcag caggaagcca aaagaaaaat tacccgtact tcttaatgaa
     5641 gctccggatg gcagggtact taaggccggt cttataggat gcggcggaag gggtaccggt
     5701 gcggcagtca attttctgga tgccggcccc aatcttgaga tcactgccct tggtgatatt
     5761 tttcaggaca agatagacaa ctgc
//
LOCUS       DAOSPX010000205         5774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12673_length_5774_cov_3.535408, whole genome shotgun sequence.
ACCESSION   DAOSPX010000205 DAOSPX010000000
VERSION     DAOSPX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12673_length_5774_cov_3.535408"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..2143)
                     /locus_tag="PLZ75_09840"
     CDS             complement(83..2143)
                     /locus_tag="PLZ75_09840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOS72732.1"
                     /translation="MNKYLLPAVLAAVLAIASCRDKTGEGRDNGSIRFSNELTREEKS
                     AGKMTPEILWKFGRLGSFDLSPDGKEILYTVTDIDVKTEARRTNIFRMPSSGGDPVLV
                     SEGGSSPRWFDNGRSIAFVKNGRLMAMKADGSEMTEVEGLDNFESYSISPAGDRILFT
                     RRVKLDRTANEKYNLPKARVRIIDDLMYRHWNYWSDYSYSHIFVASFNGKRVSGEKDI
                     MEGQRYESPTAPFFDDSEIAWSPDGRYIAYTSKRMTGRDYARSTNTDIFLYDISSGKE
                     INISEKNHGYDKYPVFSPDGSKIAFQSMERDGYESDLERLFIYDISSGKAVRLNRSWD
                     YDVENINWAGNSSLYFTCAYHGTIQIFRTDTGGGEVVRVTEGVHDLGPVMLRSGILVS
                     GLQSMSMAPELAAVDLSTGSVKQISFINKNIYDAVKMGRTEEKHIRTKDNEDMHMWVI
                     YPPDFDPSRKYPALLFCKGGPQGPLSQSWSYRWNYQLMAARGYIVIAPNRRGSSGFGQ
                     KWKEQISGDYGGKNIQDYLDATDAMAREPYVDKNRIGAVGASYGGTSVFYLAGMHGGR
                     FNAFISHCGMYNFVSWYGSTEELWFADKDIEGPYWNLPKSYQYSPHLMAANWDTPILI
                     IVGANDYRIPYTQSLEAFTAAQLNNVPSRLLFFEDEGHWILRPQNAMIWQNEFFSWLD
                     TYLQ"
     gene            complement(2161..3171)
                     /gene="recA"
                     /locus_tag="PLZ75_09845"
     CDS             complement(2161..3171)
                     /gene="recA"
                     /locus_tag="PLZ75_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOS72733.1"
                     /translation="MNNTRKEIDKEKLKALEVTIGKIEKDFGKGTIMKLGDQAIENIQ
                     VIPTGSIGLDAALGIGGLPRGRVIEIYGPEASGKTTLAIHAIAEAQKNGGIAAIIDAE
                     HTFDRNYAEKLGVDVENLLISQPDNGEQALEITDNLIRSGALDIVVIDSVAALTPKAE
                     IEGEMGDSKMGLQARLMSQALRKLTANISKTNTSCIFINQLREKIGIMFGNPETTTGG
                     NALKFYSSVRLDIRKTSQLKDGEEIIGSRTRVKIVKNKLAPPFKKADFDILYGEGISQ
                     LGEIIDLGTDFNIIRKSGSWYSYNETRIGQGREAVKQILRDNPELYDELKTKVTEALK
                     NQ"
     gene            complement(3183..3644)
                     /gene="bcp"
                     /locus_tag="PLZ75_09850"
     CDS             complement(3183..3644)
                     /gene="bcp"
                     /locus_tag="PLZ75_09850"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOS72734.1"
                     /translation="MGELKEGMKAPSFEGRDQDGNIVRLSDFKGRKLVLYFYPKDNTP
                     GCTAQACNLRDNYDSLLNEGFAVVGISKDSEASHKKFAGKYSLPFPLIADTSKKIHED
                     YGVWKQKSLYGKTFFGTARTTFIIDEEGTIEKIISRVNTREHSGQILKTLK"
     gene            3786..4772
                     /locus_tag="PLZ75_09855"
     CDS             3786..4772
                     /locus_tag="PLZ75_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOS72735.1"
                     /translation="MKPDKEQWRLAEGCSGRKSASLFIFLTALVFLSFTLVRNSDAQV
                     NPGSFRKGDSLFSDLQYAGFPAGGESGGLFGSGQEIAVHNHVAKAVSQQSLSGVLALF
                     RTDAPVLHDKAEKIEINPVHPPGLIYRIQVAVFRNPVTTEYFKGLAPVYGIRNPGTGM
                     TTYYAGMFRKADDARAALVQVREKGFRDAFLVPFSGGRPVTMERARILEKEWGVIPFT
                     PDEAVVKDIEADTVPPTLSFRVEVIRSMKPVGEGVYDEMKKLSGSRGLDVETLPDGMI
                     VYLIGNFITYQSAVDYADLLVRNGYREAKVAAWLGKKEIPVETAKQLFNSLK"
     gene            complement(4774..>5774)
                     /locus_tag="PLZ75_09860"
     CDS             complement(4774..>5774)
                     /locus_tag="PLZ75_09860"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOS72736.1"
                     /translation="TLFQEWQTTAEAKVMERSFYEVATMPFPRNKSGFVLSRRIEDGS
                     FSKIYETLIDPDDYFIRKEKPAGVEATKIIYSGDPAVNVDLAFIAEGYTAAEMPKFRE
                     DVKRISGILLSEAPFNEYRDKFNIWAVEAVSAESGTDIPGEGIYVSTILNSSFYTFDL
                     DRYLTTLDIKTVNDLAAVVPHDNIIILINSDRYGGGGVYNYYSCTTTDNPLSDHVFIH
                     ELGHGFAGLADEYYSSTVSYENFYAFDVEPWEPNITTLVDFGSKWKNMIAGGTPVPTP
                     ANAGYRGKVGVFEGAGYSARGIYRPQMECTMKSSTPSGFCAVCRKAVREMIEFYIK"
BASE COUNT         1304 a         1377 c         1357 g         1736 t
ORIGIN      
        1 atccactgga ttgacggaga actgctggcg gatgaaagag ccgcggagat actgcgtttg
       61 ctgaagggtt aatacagtcc ttttactgaa ggtaagtatc cagccagctg aagaattcat
      121 tctgccagat catggcattc tggggtctga gtatccagtg tccttcgtct tcgaagaaca
      181 gcagccttga gggcacgtta ttcagctggg ctgcagtgaa ggcttccagg ctttgtgtat
      241 acggaatcct gtaatcgttt gcaccgacaa ttatcaggat gggcgtatcc cagtttgctg
      301 ccatcagatg cggggaatac tggtagcttt taggcaggtt ccagtaggga ccttctatat
      361 ccttgtcggc aaaccacagt tcttcggttg atccgtacca gctgacgaaa ttatacatgc
      421 cgcagtgtga aatgaaggcg ttgaatcttc ccccgtgcat tcctgcaagg tagaagacgg
      481 aagtgcctcc gtagctggca cccacggcac caatccggtt tttgtcgaca tagggttccc
      541 ttgccattgc atcggtggca tcaagatagt cctggatatt tttcccgccg tagtcccctg
      601 agatctgttc cttccatttt tgtccgaagc ctgagctgcc ccgcctgttg ggggcaatga
      661 cgatatatcc ccttgctgcc atcagctggt agttccagcg gtatgaccag ctctggctca
      721 gcggaccctg ggggccgccc ttgcagaaaa gcaatgcagg atatttccgt gaggggtcaa
      781 aatcgggcgg atagattacc cacatatgca tatcctcatt gtctttggtt cttatgtgtt
      841 tttcctccgt ccttcccatt ttaaccgcat cgtaaatgtt tttgttgatg aaacttattt
      901 gtttcaccga tccggttgaa agatcgacag ccgccagttc cggagccatg gacattgact
      961 gcagtccgga gacaagaatt cccgatctca gcattaccgg tccaaggtca tgtacgcctt
     1021 ctgtcactct gacaacctcc ccgccgccgg tatctgtcct gaatatctgt atcgttccat
     1081 ggtaggcgca tgtgaaatac agggaggaat tgccggccca gttaatattt tccacgtcat
     1141 aatcccatga tctgttaagc ctgacggctt ttcccgaact gatgtcatag atgaacagac
     1201 gttccagatc tgattcatat ccatcccttt ccatactctg gaatgcgatt ttggaaccgt
     1261 cgggcgagaa caccgggtat ttgtcgtagc cgtgattttt ttccgagatg tttatttcct
     1321 ttcccgatga aatatcatat aggaaaatgt cggtattcgt gctgcgggca taatctcttc
     1381 cggtcattct ttttgaggta taggcaatat atctgccgtc ggggctccag gctatttcac
     1441 tgtcgtcaaa gaatggtgcg gtgggagact catatctctg cccttccatt atatcctttt
     1501 cccccgatac tctctttccg ttgaatgaag ccacgaaaat atgactgtag gaataatcgc
     1561 tccagtagtt ccagtgccgg tacatcaggt cgtcgattat cctcaccctt gcttttggga
     1621 ggttatattt ttcatttgcc gtcctgtcga gtttcaccct tctggtaaag agaatcctgt
     1681 ctccggcagg agaaatgctg tagctttcaa aattatccag gccttccact tccgtcattt
     1741 ccgatccgtc agctttcatt gccatcagcc ggccgttctt aacaaaggct attgaacggc
     1801 cgttgtcgaa ccacctgggt gaacttcctc cttcgctgac caggacggga tcgcctccgg
     1861 atgaaggcat tctgaaaatg tttgtcctcc ttgcttcagt ttttacatca atatccgtta
     1921 cggtatacag tatttccttt ccgtcgggtg aaaggtcgaa agatcccagg cggccgaatt
     1981 tccagagtat ttccggagtc attttccctg cagatttttc ctccctggtc agttcgttag
     2041 agaagcggat actgccgtta tcccttcctt ccccggtttt gtccctgcat gaagcaatag
     2101 caagtacagc cgcaagaact gcgggcaaaa gatatttatt catcggaatg atattttaaa
     2161 ttattgattt ttaagtgctt cagtaacctt tgttttaagt tcatcataca gttccggatt
     2221 atccctgagg atctgcttca ctgcctctct tccctggcct atcctggttt cattatagct
     2281 gtaccacgac ccgctttttc tgataatgtt aaagtctgtt ccgaggtcaa tgatttcgcc
     2341 aagctgcgat attccctcgc cgtacagaat atcaaaatcg gctttcttga atggcggcgc
     2401 aagtttattc ttgacaatct tgactcttgt cctgctgccg attatttcct caccgtcctt
     2461 cagctggctt gtctttctta tgtcgagtct cactgaggaa tagaacttaa gtgcatttcc
     2521 tcctgttgtg gtttcggggt tcccgaacat gatcccgatt ttttccctga gctggttgat
     2581 gaagatgcag gaggtgttgg ttttacttat attggctgta agttttctca gggcctgtga
     2641 catcagtctg gcctggaggc ccatttttga atctcccatt tcgccttcga tttcggcttt
     2701 gggggtcagg gctgcgaccg agtcaatcac aactatatcg agggccccgg agcgtataag
     2761 attatcggtt atttcgaggg cctgttcccc gttatccggt tgtgagataa ggaggttttc
     2821 gacatccacc ccgagttttt ccgcatagtt tctgtcgaag gtatgttccg cgtcgatgat
     2881 tgctgcaatt cccccgtttt tctgtgcttc ggcgattgca tgtattgcca gggtggtttt
     2941 tccggaagct tccggaccat agatttctat aacccgtccg cgcggcagtc ccccgatacc
     3001 gagagctgca tcgagaccta ttgatccggt cgggatgacc tggatatttt caatggcctg
     3061 atcgccaagc ttcattatcg tacccttgcc gaaatccttc tcaatcttgc ctattgttac
     3121 ctccagggct ttaagctttt ccttgtcaat ttcttttctt gtgttattca tattgctgat
     3181 tgttacttta atgtttttaa aatttgtccg gagtgttccc tggtgttgac tctggaaatt
     3241 attttttcaa ttgtcccctc ttcgtcaatt atgaatgtgg ttcttgctgt tccgaaaaag
     3301 gttttgccgt aaaggctctt ttgtttccac actccgtaat cctcatgtat cttttttgat
     3361 gtgtctgcaa tgagcgggaa tgggagggaa tattttcccg cgaatttttt atgggaagct
     3421 tcactgtcct tgcttattcc gacaacggca aatccctcat tcaggagact gtcatagttg
     3481 tccctcagat tgcatgcctg tgcagtacat cccggagtat tatccttcgg ataaaaatac
     3541 agcaccagct tcctgccctt aaaatctgaa agtctgacga tattgccatc ctgatctctt
     3601 ccttcaaatg aaggtgcttt cattccttct tttaattcac ccatgggttt ggtaattttg
     3661 ttattacggg taaatttacg aagtttgaat cgtattccta tagggaagct gatattttgt
     3721 tattaattgc aataacagaa cagatcccgg ccggaaatct gtaaataaat gaaaattaat
     3781 atattgtgaa accggataaa gaacaatgga gattagctga gggctgttcc ggaaggaaat
     3841 cagcgtcact ttttattttt ctgacagccc tggtatttct ttcgtttacc ctggtccgga
     3901 attcagacgc gcaggttaat cccggttctt tccggaaagg agattcattg ttcagtgatc
     3961 ttcagtatgc aggattccct gcaggcggtg aaagcggtgg tctcttcggc agcggacagg
     4021 aaattgctgt tcacaaccat gttgcaaaag ctgtcagtca gcagtcgctg tcgggtgtcc
     4081 ttgccttgtt caggacggat gcaccggtgc ttcacgacaa ggcggagaag attgaaataa
     4141 atccggttca tccgccgggt ctcatataca ggatacaggt ggctgttttc cgtaatccgg
     4201 taaccactga atatttcaag ggtcttgccc cggtatatgg cataaggaat cccggaaccg
     4261 gaatgacaac ctattatgca ggtatgttcc gcaaggcgga tgatgcaagg gctgcactcg
     4321 tgcaggtaag agagaagggt ttcagggatg cttttcttgt acccttttcc ggaggcaggc
     4381 ccgttacaat ggaaagggca aggattcttg agaaggagtg gggcgttatt cccttcactc
     4441 cggatgaagc tgtggtgaag gacatagagg cggatacagt tcctcccaca ttgtctttca
     4501 gggttgaggt gatacgcagc atgaagcccg tgggagaggg ggtatatgat gaaatgaaga
     4561 agctttccgg atcacgcgga cttgatgttg aaacccttcc tgacggcatg attgtctatc
     4621 ttatagggaa tttcattacc tatcagagtg ccgtggatta tgctgatctt ctggtcagga
     4681 acggttaccg cgaagctaag gttgctgcct ggctgggaaa gaaggagatt cccgttgaaa
     4741 cggcaaagca gctcttcaac agcctgaaat gatttattta atataaaact ctatcatttc
     4801 ccttacggct tttcggcaga cagcacaaaa gcctgaagga gtactgcttt tcattgtgca
     4861 ttccatctgg gggcgataga ttcccctggc tgaatagccg gcaccctcaa aaacaccaac
     4921 cttgcccctg tatccggcat ttgccggggt cggcaccgga gtgccccctg caatcatatt
     4981 cttccatttg ctgccgaaat ccaccagcgt tgttatattg ggctcccagg gttccacatc
     5041 aaaggcatag aaattttcat aggaaactgt tgaggaatag tattcatcag ccagtcctgc
     5101 aaatccatgt cccagttcat ggatgaacac gtgatctgac aaggggttat ccgtggttgt
     5161 acagctgtag tagttataga ctccgccacc gccgtacctg tcgctgttga tgagtatgat
     5221 tatattgtcg tgcggcacaa cggcggcaag atcattcacg gtctttatgt ccagggtagt
     5281 caggtaacgg tcaaggtcaa aggtgtaaaa acttgagttc agtatggtgg aaacatagat
     5341 tccttcaccg ggtatatcag tacccgattc tgctgaaact gcctccacgg cccagatatt
     5401 gaatttatcc ctgtattcat taaaaggtgc ttccgaaaga aggataccgc ttattcgttt
     5461 cacatcctcc ctgaatttcg gcatttcagc ggctgtatag ccttccgcga tgaaggccag
     5521 atcgacattc acggcaggat cccccgaata aatgatttta gttgcctcca caccggcagg
     5581 tttttccttt ctgataaaat aatcatccgg atctatcagt gtttcgtaga tttttgaaaa
     5641 tgagccatct tcaatacggc ggctcagcac gaaccctgat ttgttcctgg gaaatggcat
     5701 ggtagccact tcatagaagc ttcgttccat tacttttgct tccgcggtgg tctgccattc
     5761 ctgaaagagg gtgc
//
LOCUS       DAOSPX010000206         5713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12844_length_5713_cov_4.227466, whole genome shotgun sequence.
ACCESSION   DAOSPX010000206 DAOSPX010000000
VERSION     DAOSPX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12844_length_5713_cov_4.227466"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="PLZ75_09865"
     CDS             complement(<1..1361)
                     /locus_tag="PLZ75_09865"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS72737.1"
                     /translation="METFILYMAKSALWLTGFAAVYLLFLRNERFFLLNRLYLLTGII
                     VSFAFPFITIHYQVEVPAGSQNGIVSVLPVTEDAIAEAKGNMSGTSLLSAVMFIIYLS
                     GVLFLLYRILWHLLLLRDTIRRAGVNCRGSIKLVRVSGFPSSFSFFNYIFIDPAVGED
                     EAEEIMNHELAHVRQNHWFDLLLCELLRAVQWINPFSWIYSGLIRQNHEYLADEQALQ
                     VSANPALYRAALINQLFNSKVISLTNSFNYSLNKKRFHMMKKTETSPFRKFKVLLILP
                     VFAIILYSFSEPEYRYININENPGAGNPVAGILSEGSKAADKGSSDALPVGSGISTGE
                     AVQKTGAGTEVIIPVSAIMPEGLRGVIVRKDGKPLEGAVIVIKGTTTGTISDNKGFFQ
                     LDNVPDDAVLVVSYVGFKTMVLKPVFGSGMKISMVQDTIKVLNNEAGIPPPPPPPPPP
                     PPPP"
     gene            complement(1361..1738)
                     /locus_tag="PLZ75_09870"
     CDS             complement(1361..1738)
                     /locus_tag="PLZ75_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOS72738.1"
                     /translation="MRLTRAEEQVMQILWDLGEGVVRDIRDRFEDPKPARNTVSTVVR
                     VLEKKGFVKHKAYGNVYVYFPVISKDEYSGKQLFRLLESYFDNSFPAMASFFAREKDL
                     SIGELDELLEDTKKELRMKNKEK"
     gene            2005..2742
                     /locus_tag="PLZ75_09875"
     CDS             2005..2742
                     /locus_tag="PLZ75_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS72739.1"
                     /translation="MKKRIIYLFSVTLIFFITLASCAEGPNKLTGQEKKDGWTLLFNG
                     KDFTGWRQYGGSGMPSNWAIEDNAMKVFLAEGKREGQGAGGDIIYASREFRNFELSID
                     WKTAPKGNSGIFYYVKETPGSAIYSASPEIQVLDNENASDNKIDSHLAGSLYDMLPAI
                     PQNARPAGEWNTIVIRVKDGRVTHTQNGEKVVEYELWTPGWDKLVENSKFRNFPGFTA
                     GIPREGYIGLQDHGYTIWFRNIKIREL"
     gene            2904..5582
                     /locus_tag="PLZ75_09880"
     CDS             2904..5582
                     /locus_tag="PLZ75_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72740.1"
                     /translation="MKRKITWLAGAILFALSSFLNSSTAQQLTDYNYRGSLDVLSNTV
                     SNDVQFNGYTNHWWNDYKKWYRYGSLYKIVIPDVERKIIQSRIDMAEDMNLPGLWMQE
                     GFLTGWLEGQCTLLDNPSPAELSAAAAGGNVLVITSPASETGKILHAKYPGNEKWKRI
                     LKSYQFNDPFLTVIDAFILENGNRKIFVISSGSHAAALKVKDLMENVKSVLSKYDLHK
                     GWFATETLLKSVTCTPGHPLEIIGRGMNEGNSWFVFNGYMDFLMKDELDDWLSRTKLP
                     VVGDVGFSPVFGLDNYDGLQVQDMPDRQSWIDFAHSRNGYVFRPVWDPGSDPYHYDGY
                     IATAGNKLQIDNENVPFIVNTGGLLNNLVPSMVLFIDKGEKLTKKQLFDAIMARREVG
                     ITQEGIVMGPAEFRTAMQMLFLDRVYLENYFGDRIDLDAVMDGYTLSFSISNTYDQPV
                     TGTWDVQLPEGIQTDESLSSSVTIPAGGSTTVRVNIRPGAGGMDYPNPIVAGFKWGGN
                     EKYTMCVMEMPPAISVHKLIYGHAPVVSFPVSIHNFSGESRYPVTVRVIDGKRPSKPV
                     FTSTQYGTNPTGTYTDLRFDLKVPAGNYEVEVTALGIKAFSQLGVGRASGKVYAYATD
                     LNNDGIDEFRLENDSVRITLLATGARVIEYIVKSRNDNVLFKLWPEKSGDDKRPNRLW
                     GYYPYGGFEDFLGQASMETHKVYDAELVKSEGDYVQVRMSADYYGNKLEKTFTLYGNS
                     PLLEVRYALKFINPEANVIGPQPILELGESHWTEDVFITPTIGGREELRMRPEDYYGR
                     ALMIEEGWNAGYDTKEDIAFIGAFPVEQPIFLHMWMNHPRNSDAHHYYVEFQPWTPIY
                     QKNTMYFTYYLWGSGGKWESALKEMRNRNLISVRKK"
BASE COUNT         1712 a         1289 c         1284 g         1428 t
ORIGIN      
        1 ggtggaggag gcggaggcgg tggaggcggt ggagggggag gaataccggc ttcattattc
       61 aataccttta tagtgtcctg aaccattgat atcttcattc cggagccaaa tacaggcttc
      121 agtaccatcg ttttgaatcc cacataactc acaacaagta cggcatcatc cggaacattg
      181 tcaagctgaa aaaatccctt gttatccgaa attgttccgg ttgttgttcc ttttatcact
      241 attacagcac cttcaagcgg ctttccgtcc ttccggacaa ttacccctct taatccttca
      301 ggcatgatgg ctgaaacggg aattatcacc tctgtcccgg ccccggtttt ctgtacggct
      361 tctcctgttg aaataccgga tcctacggga agggcatcac tgcttccttt atccgcagcc
      421 ttgcttcctt cagataagat ccctgcaacc ggattacctg cacccggatt ttcatttata
      481 ttaatatatc tgtactcagg ttctgagaat gaatacagga taattgcaaa aacaggaaga
      541 atcaacagta ctttgaactt cctgaatggt gaggtttctg tttttttcat catgtgaaac
      601 cgttttttgt taagtgaata attaaatgag tttgtaagac tgatgacctt tgaattaaac
      661 agctgattta tcaaagctgc cctgtagaga gccggatttg ccgagacctg aagggcttgt
      721 tcatcagcaa gatattcatg gttctgcctt ataagaccgg aatatatcca gctgaacgga
      781 tttatccatt gcactgcacg cagcaattca cataataata ggtcaaacca gtgattctga
      841 cggacatgag ccagttcgtg gttcataatc tcctcagctt catcttctcc aacagcagga
      901 tcaatgaaaa tatagttgaa gaatgagaat gacgagggaa atcccgaaac cctgacaagc
      961 tttatggatc cacggcaatt aacacctgct ctgcggattg tatccctgag caggagaaga
     1021 tgccataaaa tcctgtataa cagaaataat acaccggaaa gatagatgat gaacattact
     1081 gctgacagaa gggaagtacc ggacatattc ccttttgcct ctgctatcgc atcttccgta
     1141 accggcaaca ctgatactat cccgttttgt gagcctgccg gaacttccac ctgatagtga
     1201 atggtaataa acgggaaagc aaatgaaact ataatgccgg taagaagata caaacggttc
     1261 agtaaaaaaa atctttcgtt ccgcagaaat aaaagataaa cagcagcaaa tcctgtcagc
     1321 cacagtgccg actttgccat atacaatata aacgtttcca ttatttttcc ttattcttca
     1381 tccgaagctc ctttttcgta tcttcaagca gttcatccag ttcccctatc gaaagatctt
     1441 tttctcttgc aaagaatgat gccattgcag gaaatgagtt atcaaaatag ctttccagca
     1501 gtctgaaaag ctgtttcccg gaatattcgt ctttcgaaat gacaggaaaa taaacataaa
     1561 catttccata agccttatgc tttacaaatc ccttcttttc caggaccctg acaacagttg
     1621 aaactgtatt ccgtgcaggc ttgggatcct cgaaacggtc acgtatatcc ctgaccactc
     1681 cttcacccag atcccacaat atctgcataa cctgttcctc agcgcgtgta agtctcattt
     1741 tatatcaaat aattatggta tgacatcata acaaatcagg acataacaaa tatatgacta
     1801 ttttgatagt catgcaaatt ttttttaata aaaatgacta cgattttagt cgtatttgta
     1861 attgcctgat ctgactgttc cgtctgacat gttttcctgg ttttcataga aagaatatcc
     1921 aggacttcag tatttggttc atttattttc gatgaacaaa taattttacc taatttgcag
     1981 aacagaatct aaaacttgta aaccatgaag aaacgtatta tttatctttt ttcagtgacg
     2041 cttatttttt tcatcactct cgcgtcatgt gcagaaggtc ccaataaact caccggacag
     2101 gaaaagaaag acggctggac acttctcttc aacggtaagg atttcaccgg atggaggcag
     2161 tacggcggca gcgggatgcc ctcaaactgg gccattgaag acaatgccat gaaggtgttt
     2221 ttagcagaag ggaaaagaga aggacagggt gcaggcggag acataatcta tgcatccagg
     2281 gaattcagga atttcgaact gtcaattgac tggaaaacag caccgaaggg caactcaggc
     2341 attttctact atgtgaagga aactcccgga tcggccatat actctgcatc acccgagata
     2401 caggtactgg acaatgaaaa tgcaagcgac aataaaatcg acagtcacct ggcaggttct
     2461 ctctatgaca tgcttcccgc cataccacaa aacgccaggc ccgcgggtga atggaacacc
     2521 attgtcatcc gggttaagga cggcagggtg acacataccc agaacggtga gaaagtggtg
     2581 gaatacgaac tgtggactcc cggatgggat aaactggtcg aaaacagcaa attcaggaat
     2641 tttcccggat tcacagcggg aattccaaga gagggctata taggacttca ggaccacggc
     2701 tacacaatct ggttcaggaa tatcaagata agggagttgt aacagccccg gattccgttt
     2761 gcaccttccc ggtcatatac tgtccggttc cggtcacaaa cagaattccc gtctgaacgg
     2821 gatctgctga aatctaaata attgtcacaa aattcaagaa tgttatcccg tgtaacggat
     2881 acatcaaaaa acctaatgat tatatgaaaa ggaaaatcac atggctcgca ggcgcaatac
     2941 tttttgccct ttcctctttc ttgaacagtt caacggcaca gcagctgaca gactataact
     3001 acagggggtc actcgacgtt ctgagcaata cagtcagcaa cgacgttcag ttcaacggct
     3061 atacaaacca ctggtggaat gattacaaga aatggtacag atacggaagc ctttataaaa
     3121 tagtaatccc cgatgttgaa agaaaaatta tccagagcag gattgacatg gcagaagaca
     3181 tgaaccttcc gggtctctgg atgcaggaag gttttcttac aggctggctt gagggacaat
     3241 gcaccctgct tgacaacccg tccccggcag agctctcggc tgctgccgcc ggaggcaacg
     3301 tactggtgat cacttctccc gcatcggaaa cgggaaagat acttcatgca aaatatccgg
     3361 gtaatgaaaa atggaagcgg atcctgaaaa gctatcagtt caatgatcct ttcctgacag
     3421 tgatagacgc attcatactc gaaaacggaa accggaaaat attcgtgatc tcttccggca
     3481 gtcatgcagc ggcactgaaa gtaaaagacc tgatggaaaa tgtaaaatct gttctttcaa
     3541 aatatgatct gcacaaggga tggtttgcca ctgaaactct cctgaaatca gtgacctgca
     3601 cacccggaca tcctcttgaa ataatcggca ggggaatgaa tgagggcaac tcctggtttg
     3661 tcttcaacgg ttatatggat ttcctaatga aggatgaact ggatgactgg ctctcaagaa
     3721 caaaactgcc ggtagtagga gacgtcggct tctctcccgt tttcggactc gacaactatg
     3781 acggacttca ggtgcaggac atgcccgaca ggcagtcatg gatagatttt gcacattcca
     3841 ggaacggata cgtcttccgt cctgtctggg acccgggaag cgatccctat cactatgacg
     3901 gctacatagc cacggcaggc aacaagctgc agatagacaa cgagaatgtt ccgttcatag
     3961 taaataccgg cggactgctg aataatcttg tcccttcaat ggtcctgttc atagacaagg
     4021 gtgaaaagct tacaaaaaag cagctctttg atgccatcat ggcacggaga gaggtgggca
     4081 ttacacagga aggaatcgtg atgggtcctg ctgagttcag aacggcaatg cagatgctct
     4141 tcctcgacag ggtatacctg gaaaattatt tcggtgaccg gatcgatctg gatgcggtaa
     4201 tggacggtta taccctcagc ttcagcatca gcaacactta tgaccagccc gtgaccggga
     4261 catgggacgt tcagcttccc gagggaatac agacagatga atcactttca tcatcggtaa
     4321 cgattccggc cgggggcagt acgactgtcc gcgtgaacat aaggcccggt gccgggggaa
     4381 tggattatcc caatcccatt gtagccggtt tcaaatgggg cggaaatgag aagtacacca
     4441 tgtgcgtaat ggaaatgcct cccgccattt ccgttcataa acttatctac ggacatgcac
     4501 cggtggtatc attccccgta agcatccata acttcagcgg ggaaagcagg tatccggtaa
     4561 ctgtaagggt gattgacggc aaaaggccgt caaagcctgt attcacctca acacagtacg
     4621 gaacaaatcc cacgggaaca tatacagatc tcagatttga ccttaaagtg cctgcaggaa
     4681 actacgaagt ggaagtgact gccctgggaa taaaagcatt cagtcagctc ggtgtcggca
     4741 gggcttcagg aaaagtatat gcctatgcca ccgaccttaa taatgacggg atagatgaat
     4801 tcaggcttga aaacgacagt gtaagaataa cacttcttgc aacaggggca agagtgattg
     4861 aatatattgt aaagagccgc aatgataatg tcctcttcaa gctatggccc gaaaagtccg
     4921 gagatgacaa acgtccgaac cgtttgtggg gatattaccc ctatggcggt tttgaggatt
     4981 tcctcggcca ggcaagtatg gaaacccata aggtttatga tgccgagctt gtaaaaagcg
     5041 aaggagacta tgtacaggtg agaatgagcg ccgattatta cgggaacaag ctggaaaaga
     5101 ccttcaccct ttatgggaat tctccccttc ttgaggtgcg ttatgccctg aagttcatca
     5161 atcccgaagc caatgtcatc gggccccagc ctattctgga actgggagaa tcacactgga
     5221 ccgaagacgt gttcattaca cccacaatag gaggccgtga ggaactccgg atgcggccgg
     5281 aggactatta cggaagggca ttaatgattg aggaaggctg gaacgcaggc tacgacacga
     5341 aagaagatat agccttcata ggtgccttcc ctgttgaaca accaatcttc cttcatatgt
     5401 ggatgaatca tccccggaac agtgatgcac accattatta tgttgaattc cagccatgga
     5461 caccgatata tcagaaaaat acaatgtatt ttacctatta cctgtgggga tcgggaggca
     5521 aatgggaatc agcccttaag gaaatgagaa acagaaacct gatatcagta cgtaaaaaat
     5581 aaaaccatca gaaaatgaag tcatcaaaaa aaacagcagt tgtattacta ttggcacttc
     5641 tcctgattct tccgcttaca ggacaagtgg caaagcaaaa ggaatccctg cagataaaga
     5701 atattgaatt act
//
LOCUS       DAOSPX010000207         5689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12918_length_5689_cov_8.388534, whole genome shotgun sequence.
ACCESSION   DAOSPX010000207 DAOSPX010000000
VERSION     DAOSPX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12918_length_5689_cov_8.388534"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..593)
                     /locus_tag="PLZ75_09885"
                     /pseudo
     CDS             complement(78..593)
                     /locus_tag="PLZ75_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(615..2270)
                     /locus_tag="PLZ75_09890"
     CDS             complement(615..2270)
                     /locus_tag="PLZ75_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72741.1"
                     /translation="MKETYGEYLFDAVSGSITECLGGITEEDVRDCCDFKIYRRGQEY
                     YEEGMVEELMHNKAINTVIATVKGTREYQIEFRLEEGGIYSTCNCPYDGVCKHTVAVL
                     LNLIHEGTDNIATYTLNCPTTAESLDFLKKYLETLSKKDLVLLTMKFAPANFIKEVRN
                     REMPANDAEAIFRKSEKKIKKFFEDTDLLYDPGGMESALISQLNNLKGLENQQRDGIG
                     ELILFIIRSVETAFNEGYLYIDDYYGDEYFESDDFCEYVIAYVKQLPFEVKTNYLKKL
                     DQALNEMSYDTFSTIQESYHRFFSEHERKDLKSFVKLDGEIPQTMLSRLYKFLEPELS
                     SDEKEAILRVISRSDTDHFLSFCRQLSDQNRYPEVIDLIKDDSDGFKPLHDFRIAEIY
                     LEAAHKLNMNMDEISEEVVKHCPEVSILRKIKALKGTLGSNCEAIAKQKNPENLLSFY
                     EEEERMKDALDLIREPKLFYDDVIFEFYRKNHKRFPEEAETFLKRRIEEDLAYTGKKY
                     YERIAESLDLMKRINPGRSQRIADEIRANFKKRSSLIQIIRGF"
     gene            2322..3047
                     /locus_tag="PLZ75_09895"
     CDS             2322..3047
                     /locus_tag="PLZ75_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72742.1"
                     /translation="MNREAQKIIKELYPKFPEWDRDFREFLGLFYRDILIPETDEQKI
                     WDSMENIYATVLESIIRMTGISDKWDGPEFLPLAVNAGLEVFYRSDKLKCPFYFGTDE
                     NGFFLSADMIYSENIRKMDDRFWFQMAELTRFGKLDLWENRVFPESQVRKEPWFHRKS
                     KSRIFHLIRSAVAWEKEEGFAEDLGMIIIRWKYDTAWEELLCKGSAAFHNLYRINEAL
                     WKKEFSGTTRNRGRLNKGLTCDK"
     gene            3322..4383
                     /locus_tag="PLZ75_09900"
     CDS             3322..4383
                     /locus_tag="PLZ75_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOS72743.1"
                     /translation="MKSIASALVALFILTGICAFRTGENNKASDGNQNGRSGVAVIMT
                     GAAARIPQEAALLEELDNRGLFKDVVFISGVSSGALNSVMLNAVLSGKLSWEEYRNIL
                     FGLKNSDIFTQGQKKIPVNSAPERELFRRIVEDGLGYETIGDLPFTTAISFTHLQELG
                     VKKNVYRMCSRKINPESDTTLSLVDIMMASSAFPVAFPPVRIKNARTIPDVEYVDGGV
                     GEDHVPYHALLQFEKFKGKGVDKVYIISRKCDSIPEVSEELMNLGINDRGIFDRLGIS
                     LDEILEKGIIKRLKAYASEAPELASRTFVWMPDFKADFLMFDFSNLQEQYEMTSQWAR
                     INEPIPLADFFLAAEKKAR"
     gene            4454..4765
                     /locus_tag="PLZ75_09905"
     CDS             4454..4765
                     /locus_tag="PLZ75_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72744.1"
                     /translation="MKRLDTLFHDAGSLILFGSIIILTILAPALIDRGLMNKLVSRSK
                     ASNNDTTSIVFIRNFVRLTIYLSGIGRAYIPRLKKLCSFASCRSRCNDTDHGLFFPAD
                     T"
     gene            5283..5642
                     /locus_tag="PLZ75_09910"
     CDS             5283..5642
                     /locus_tag="PLZ75_09910"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HOS72745.1"
                     /translation="MPEILYQINPDRNVPWNNLPLLPIRKELYQTIEILEKLGDAKAA
                     LARLQGRSIVIPNPGLLINTISLQEAKISSAIENIFTTDDELYRAYSDQAIVTNGAPK
                     EVLRYKEALWSGYYYLT"
BASE COUNT         1660 a         1172 c         1143 g         1714 t
ORIGIN      
        1 agacgcatta gacaagactt tacttggtta aaaataatac ttatgatttc atatttgaaa
       61 ttatctccta gaatccgtca gatttttcgc caggtaatat gaaaatcagt gacaaacatt
      121 acaccctgaa aatcgtaagt gaatgataat gaaaaccagg acaaaccgac aaactctgaa
      181 aaacaaggtt ttatctcact ccttgttatg ccaacatttg aatggttgaa gatttgggac
      241 agatatacca agctcctttc actctgtcca tccatttccc acactcgttt tccgaatgtt
      301 ttccgtaaag tatgggttga agcattttga atttttatcc catatgtttt cagggcgctt
      361 tttagaagcc tgtttgcata ctggattgta aatttttgat cctttctatt ggcaaaaagg
      421 taattttcaa ttttaaacct gcctgttaat gagtatttgg tggatgaata cttaagaacc
      481 tctttaagag attcatttac ggttatgctc cttactttcc ccgttttgct ttctgtgata
      541 gtaatttcat ctttgccaag tatatcaatc cacctgatat tcaaaaggtc cttagccctg
      601 aggccaggat atgatcagaa tccccgtata atctgtataa gactactccg ttttttaaaa
      661 tttgcacgta tctcatcagc tattcgttgt gatcgtccgg gatttatccg tttcatcaga
      721 tcgagtgatt cagcaatcct ctcataatat ttcttacctg tataggcaag atcttcttca
      781 atccttctct tcaggaaagt ttcagcttct tcagggaacc gtttatggtt cttcctgtaa
      841 aattcgaaaa ttacatcatc ataaaagagt tttggttccc ttatcagatc aagggcatct
      901 ttcatacgct cttcctcttc ataaaaactc aacagattct caggattttt ttgtttggct
      961 attgcttcac aatttgaacc gagggtgcct ttcagagctt tgatctttcg gaggattgag
     1021 acctccgggc aatgcttcac aacctcttca gaaatctcat ccatattcat attaagctta
     1081 tgtgctgctt ccagataaat ttctgctatc ctgaagtcat gcaggggttt aaatccgtct
     1141 gagtcgtctt tgatcaggtc tataacttca gggtaacggt tctggtctga taactgccgg
     1201 caaaaggata aaaaatgatc tgtatctgac cggctgatta ccctgaggat agcttccttt
     1261 tcatcagagg aaagctcagg ttccaggaat ttatacaacc gtgagagcat tgtctgagga
     1321 atttctccat ccaatttaac gaacgatttc aggtctttac gttcatgttc cgaaaagaaa
     1381 cggtggtatg attcctgtat agtggaaaat gtatcgtatg acatctcatt cagagcctga
     1441 tccagtttct tcaggtagtt cgtctttacc tcaaatggaa gttgttttac ataagcaatc
     1501 acatattcgc aaaagtcatc tgattcaaaa tattcatccc cgtaataatc gtcaatatac
     1561 aggtatcctt cattgaatgc agtttcaacg cttcggataa taaataagat caattctcca
     1621 ataccatctc tttgctggtt ttcaaggcct ttcaggttgt tcagttgtga aatcagggcg
     1681 ctctccatac caccgggatc atacaggagg tcagtatctt caaaaaactt ctttatcttt
     1741 ttttcactct ttctgaatat tgcttcagca tcatttgcag gcatttcccg gttccgaact
     1801 tccttaataa aattcgcagg agcaaacttc attgtaagaa gtaccagatc ttttttggac
     1861 agtgtttcca ggtatttctt caggaaatcc agagattcag cagttgtggg gcaattgaga
     1921 gtatatgtgg caatattatc agtaccttca tgtataagat taagaagtac tgcaactgta
     1981 tgtttacata ccccatcgta tggacagttg caggtgctgt aaatgcctcc ctcttcaagc
     2041 ctgaattcga tctggtattc tcttgtgcct tttacagtgg caataactgt attgatggct
     2101 ttattatgca tgagttcttc caccatgcct tcctcataat attcctgacc tctccggtat
     2161 attttaaaat cacagcagtc cctcacatct tcttccgtaa ttccacccag gcattccgtt
     2221 attgatcccg agacagcatc gaagagatat tctccatatg tttccttcat ttgcggttta
     2281 tttaaaattc atattcagtt taaaattaat acttttacct catgaacaga gaagcacaaa
     2341 aaataataaa agaactttac ccgaaatttc ctgaatggga ccgggatttc agggaatttc
     2401 tcgggttgtt ctatcgggat atcctgattc ctgaaacaga tgagcaaaag atatgggatt
     2461 caatggagaa tatctatgcg actgttttag aatcaataat cagaatgacc gggataagtg
     2521 ataagtggga cggccccgaa ttcttacctt tggccgttaa tgcaggactt gaagttttct
     2581 acaggtctga caaactgaaa tgccccttct attttggcac cgacgaaaac ggtttctttt
     2641 tgagtgctga tatgatctat tctgaaaaca tccggaaaat ggacgaccgg ttctggtttc
     2701 agatggcaga attaacccgt ttcgggaaac tggatctttg ggagaacaga gtctttccgg
     2761 aatcacaggt aaggaaggaa ccatggttcc accggaaatc aaagagccgg atctttcatc
     2821 tgatcaggag cgctgtagcc tgggaaaagg aggaaggttt tgcagaggac ctgggtatga
     2881 ttattattcg ctggaaatat gacactgcct gggaggagtt gctgtgtaag ggatctgctg
     2941 catttcacaa tctgtaccgg attaacgagg ctttatggaa aaaagagttt tcaggaacaa
     3001 ctcgaaacag gggcaggcta aataaaggcc tgacttgtga caaatagtgc ggcagaatat
     3061 tatgttgatt taaaaatggg gacaaatact ttccctgatt ccggaaaaca gattctgaac
     3121 cagtaatact tagtcctgca aaaacatatc agtgtgtcgg ggaaaggctc tcccgatacc
     3181 agtccgacgg gttcagctga cacgaccaca ggctgcatag aagtttatct atttaacata
     3241 cactatccca tgattccccg cactgaaaat tatctctttt cccgtaactt tattgtaatt
     3301 ttattccaat taacccgggc catgaaaagc attgcttccg cacttgttgc attattcatt
     3361 ctgacaggaa tatgcgcttt cagaacaggc gaaaataata aagcatcaga tggaaatcag
     3421 aacggccgca gcggtgttgc cgtaatcatg acaggagccg ccgccagaat cccgcaggag
     3481 gctgcattgc tcgaggaact tgacaacaga ggcctgttca aagatgtcgt attcatctcc
     3541 ggtgtgagct ccggggcgct taattcagtg atgctaaacg ctgttctaag cggtaaatta
     3601 tcgtgggagg agtaccggaa catacttttc ggcctgaaga acagtgacat tttcacccag
     3661 ggacagaaga aaatcccggt taactccgca cccgaaagag aactgttcag aaggatagtg
     3721 gaagacggtc tcggatatga aacgataggc gatctgccgt tcacaaccgc gatatcattc
     3781 actcatcttc aggaactggg tgtaaagaaa aacgtttaca ggatgtgcag ccgcaagatc
     3841 aatcccgagt ctgacacaac cctgagcctc gtcgatataa tgatggcatc atccgcattc
     3901 ccggtggcct tccctcccgt caggataaaa aatgcccgca ctattcccga cgttgaatac
     3961 gttgacgggg gagttggtga agatcatgtg ccctaccacg cactcctgca attcgaaaaa
     4021 ttcaagggaa agggcgtgga taaggtttac attataagcc ggaaatgtga cagcataccc
     4081 gaggtaagcg aagaacttat gaacctgggc attaatgaca ggggaatatt cgacaggctg
     4141 ggtatttcac ttgacgagat ccttgaaaaa ggaatcatca aaagacttaa agcctatgcc
     4201 agtgaagcac ctgagctggc ttcccgtact tttgtctgga tgcctgactt taaagcggat
     4261 tttcttatgt ttgatttcag caatcttcag gagcaatatg aaatgacttc ccagtgggcc
     4321 aggatcaatg agccgatacc tcttgccgat tttttcctgg ctgcggagaa aaaggcaaga
     4381 taatcacagg ctgaatttca aatcattaaa tttaccacca gcaactattc atttcattat
     4441 accggtggaa aaaatgaaaa gacttgatac gctgtttcat gatgccggat cacttattct
     4501 attcggttca ataatcatcc tgacaattct tgcacctgcc ctgatcgaca gggggcttat
     4561 gaataaactt gtcagcagga gcaaagcctc gaacaatgac actaccagta ttgttttcat
     4621 caggaatttc gttcgactga caatttatct ttctggtata ggccgggctt atattccccg
     4681 cctaaaaaag ctttgctcat tcgcttcttg ccggagccgg tgtaacgaca ctgaccatgg
     4741 gcttttcttc ccggcagata cttagggttt gctttcaatt tcagaaagtt ttattctaat
     4801 gtcatgaagc tctcctcttg actgaccatt gaaaatttca gagaattcat cttcatgagc
     4861 tgagtatgga agtgtttaat atttcggctt cctgttacag ataatgctta gctggaccgg
     4921 cagcttgcaa agagaaatcg cgttcttgca acaatgaaaa attagcatta accatgcatt
     4981 gaaagtggta tacttttgaa gtgaaatagt ggtatggttt ttcaaataaa gaaaacagac
     5041 acatttctaa atatcagaca cttacaagac catttgcagg tgtccttttg ttcgggacca
     5101 aataacccaa acaacattga tattggttca ttttaaggtt tgccctgact atacttcccg
     5161 gatttaagcc attaattcat tgcgaacatt aaaaatgtaa tattttaagg cttggttgtt
     5221 gtttttacac taaatatatt tgaggctgga agttgcgtaa acattaaaac atctattaat
     5281 taatgccaga aatactttat caaatcaatc ccgacagaaa tgtcccatgg aacaatcttc
     5341 ccctgttgcc aataagaaaa gaactctatc agacaataga gatattagag aaactgggag
     5401 atgctaaagc cgcattagcc aggcttcagg gtcgtagtat tgtgattcca aatccaggtt
     5461 tattaatcaa tactatcagc ttacaggaag caaaaatttc aagtgccatt gagaacattt
     5521 tcacgacaga tgatgaacta tacagagcat atagtgacca ggccattgta actaacggag
     5581 ctccaaaaga ggtgttaaga tataaagagg cactgtggtc tggttattat tatttaacct
     5641 gaattattca taagtactta aaaaaaaggg tgcttttgtt ctttaatta
//
LOCUS       DAOSPX010000208         5680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12939_length_5680_cov_4.433778, whole genome shotgun sequence.
ACCESSION   DAOSPX010000208 DAOSPX010000000
VERSION     DAOSPX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12939_length_5680_cov_4.433778"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..832
                     /locus_tag="PLZ75_09915"
     CDS             155..832
                     /locus_tag="PLZ75_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOS72746.1"
                     /translation="MKQGGTGPIAGQVVYTPPKGQLIIKEKLDNLVAFLNDDENFKMD
                     YLIKMAIAHYQFEAIHPFRDGNGRTGRIFNINYLTKRNLLDVPILFLSRYILDHQKDY
                     YSCLSGVSQRGNWKDWLLFMFKAIEYTSIITFQKINEIVSAKDSILEFIRKDARKMRN
                     PEDLVHALFTQPFTKVSHLVDSGIYAENTARDYLNRLCDIHVLEKKVIEGRHYYLNLE
                     LYRILSE"
     gene            1376..2584
                     /locus_tag="PLZ75_09920"
     CDS             1376..2584
                     /locus_tag="PLZ75_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOS72747.1"
                     /translation="MESIADRKKAREPDVPQLLKSVLLRTGDILLTYRRNVRHMMPVM
                     KIQKAVLCSLSVIIAALISCASDKENTENESLLIQEQGSFAVGGTVISNPGVFDPYNQ
                     TPAGQTFHGDHAYVFYQIPAKARKYPLVMWHGIGQFSKTWETTPDGREGYQNIFLRRR
                     FGVYLIDQPRRGDAGRSTIAGTIEPTPDEQGWFGTFRIGIWPDYFEGVQFARDQETLN
                     QYFRQMVPNIGPIDINLNVEAVSALFTKTGPGILVSHSHSGGMGWLTAIRNPNIKAIV
                     SYEPGSGFVFPEGEVPEPIPSSGGTLVAEPVPMADFLKLTKIPVVIYYGDFIPEKPDP
                     NPGQDGWRARLEMSRLWRDCVNKHGGDVTVVHLPEIGIRGNTHFPFSDLNNVEIADLI
                     SEWLKLKGLD"
     gene            2831..3574
                     /locus_tag="PLZ75_09925"
     CDS             2831..3574
                     /locus_tag="PLZ75_09925"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOS72748.1"
                     /translation="MNFTNKVVIVTGSGKGIGRSIALTYASEGAMVILAEKDPETGTD
                     AEKAIKASGFTAVFIRTDISDPEDIKRMVGETVRLFNTIDILINNAGISEWASPYELP
                     VEEWDKVINTNLRGTFLCSREAAMVMKEHGGGSIVNIASTRAFMSEPDSEAYAASKGG
                     IVALTHALAASFAGDHIRVNCISPGWIETGDYTRLRETDHKQHYSGRVGKPEDIAKAC
                     LYLTDYGNDFINGTNIIIDGGMTRKMIYE"
     gene            3889..>5680
                     /locus_tag="PLZ75_09930"
     CDS             3889..>5680
                     /locus_tag="PLZ75_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72749.1"
                     /translation="MKGIFPLLLLVSTITLHAQNPVCYVYHGCGGATVAAYPGDPQWT
                     NITYEFQRFFEGNWVTVHQTIENYHLVTPGDISLATQYRAIMRNNETLRQIISNSVIV
                     DPARFNEAVARPEPKLTFYWGTIATNWSNYLRVQPTSLPNLDGMRPPFTFTIKKKDGS
                     VVDQKLSSNLVIFDRNIEANQEYVVIVKDYCNQVDSVTGNIAFAARGRVVDVNCSGAS
                     VELYNAFVGENFNGRLPLTYGLAPLHDTILSTAVPQSIIDGISYNYSEGLITGLTEKR
                     YVLSAKDRFGVISRYSVINTGIDYPTAALRGYGSYGGFCEYYVNLMGVYPEHGVRLAG
                     TSDPYIFTPGLTANNLRLGKTYEIVVRDSCGRLSEPTIIFFPAVDPVITSVVVDTEDC
                     KTKLTVHAQTCSDTPEYMLKISRGNPGVWQPSNIFVYESITGCDTVFVRDGDKVIFQE
                     ICTGTLEADVLLYPVSDICGSPYELYVGADKGFPPYTFEISSDGINFSDPTEYAVFNW
                     LPPAIYTVRITDACGNTFVTSPEDGVAGGLFEVTESGFMGECGSSEGYLRINIDPGTD
                     IPVNYPPYKYSVKEIISGSGYNISYGSVIAS"
BASE COUNT         1744 a         1174 c         1239 g         1523 t
ORIGIN      
        1 agacccaaag gtgtatcttt acactcaatt atgagtttat gaattaatca ggttaagaaa
       61 ccaggtttac tttgatatag agtattttat taaaattttt caggaaataa aacaaacaaa
      121 agaggagata cggcctgatt ttttaaatcc aacaataaaa caaggtggta cagggccaat
      181 tgctggtcag gttgtttata ctccgccaaa gggtcagctt ataattaaag aaaagttaga
      241 caacctggta gctttcttaa acgatgatga aaatttcaag atggattatt taattaaaat
      301 ggccatagca cattatcaat ttgaagctat tcatcctttt cgtgatggca atggcagaac
      361 aggtcggatc tttaatatca attatttgac aaaaaggaac ttacttgatg tgccaatact
      421 atttttaagc cgatatattc tagatcacca gaaagattat tactcttgtt tatcaggggt
      481 ttcgcaaaga ggcaactgga aggactggct attattcatg tttaaagcta ttgaatatac
      541 atccataatt actttccaaa aaatcaatga aattgtatca gccaaagatt caattttaga
      601 gtttatcagg aaagatgcca ggaaaatgag aaatcctgaa gacttagtac atgccctgtt
      661 tactcaaccc tttactaaag taagccatct ggttgattca gggatatatg ctgaaaatac
      721 tgcaagggat tatttaaaca gattatgcga catacatgtt cttgagaaaa aggtaattga
      781 aggccgtcat tattatttga atcttgaatt ataccgcata ctatctgaat aagcattcca
      841 aaaagtaaag ttattctggt gccggtcctg atgcaagtat tttcaccaaa tcggtataaa
      901 tccctgaaat cttttacaat tattctgtta caaaataaac cagtgcagac agatacaaat
      961 ctgtccaaat ctgtggaaag cctcataaga agatcataac cagggggtcg ttggttcgat
     1021 cccaaccggg cccgccactt ccataatgag acaagcatta tacgttttgg tgtttcaata
     1081 cttctcaaag cgttaagcag atatacattc tcaaatcata gtgtttctgc tggcgcagtc
     1141 caatttgatt ccccataata ttgcggtaaa tgcaataccc tgagattcat taaatgaata
     1201 ttcagtaatt ccgatataaa atccttaatc tgcatacaga gaaaccggtt ttttaattca
     1261 gcctttatct tgttgtgcac cttttattgt aaattcacaa gggaataatc aataccaaag
     1321 ggaataatca ataccaaagg gaataatcaa taccaaataa ctaacaatac tgaagatgga
     1381 aagtatagct gacagaaaaa aagcaaggga accggacgta cctcagcttt taaaatccgt
     1441 attgctgaga accggtgata tattgttgac ctaccgcaga aatgtccggc acatgatgcc
     1501 ggtaatgaaa attcagaaag ctgttctgtg ttccttatca gtcattatcg ctgctctgat
     1561 atcatgtgca tctgataaag aaaatacaga gaacgaatca ttgctaattc aggaacaggg
     1621 tagttttgcc gtgggaggaa ccgtaatttc caatccgggc gtttttgatc cctacaatca
     1681 gaccccggca ggacaaacct tccatggtga ccacgcctat gtcttttatc agatccctgc
     1741 taaagcccgg aaatacccac tggtgatgtg gcatggaatc ggtcagtttt ccaaaacatg
     1801 ggaaacaaca cccgacggaa gggaaggcta tcagaatatt tttcttcgcc ggagatttgg
     1861 ggtatatctt attgatcagc ccagaagagg ggatgcagga cggagtacca tagccggcac
     1921 cattgaaccg acacccgacg aacagggatg gtttggcact tttcggatag gtatatggcc
     1981 cgactatttt gagggtgttc agttcgcccg tgaccaggag actctcaatc agtattttcg
     2041 gcaaatggtg cccaacatag ggccgataga cataaatctg aatgtcgaag cggtttctgc
     2101 cctgtttacc aaaacaggac cgggaatact tgtatcccat tcacattcag gaggaatggg
     2161 ctggctcacc gcaatcagga atccaaatat aaaagctatt gtctcatatg aaccgggcag
     2221 cggcttcgta tttccggaag gggaggttcc ggaaccgata cccagttcgg gaggcacact
     2281 ggtggctgaa cctgttccta tggctgattt tcttaaactg acaaaaatcc cggtagttat
     2341 atattatggt gatttcatac ctgaaaagcc tgaccccaac cccggtcagg atggctggag
     2401 agcacgcctt gaaatgtcaa ggctgtggag agactgtgtc aacaaacatg gtggcgatgt
     2461 tacagtggtg catctgccgg agatcgggat caggggcaac acccatttcc cgttctcaga
     2521 tttaaacaac gttgaaatcg ccgacctgat atcagagtgg ttaaagctga aaggactgga
     2581 ttaaaccggg gaaaattcag ggtaccagct tataattatt taagaaagtt catacgggac
     2641 taaataacaa ccgataagct taatttgaaa accacacgga aaatcacgaa cttcgttttc
     2701 agctgatgta tgccaacaac ggcctttctc agaaagaact tacagccagg aagtctgccg
     2761 gtcagatgaa aacagccagt gtgtcggatt acgagataag gaagattctt acatcaatca
     2821 gatttgatct atgaacttta cgaataaagt agttattgtt acaggttcag gcaaaggcat
     2881 cggccgtagt attgccctga cctatgcatc ggaaggtgca atggttattc ttgctgaaaa
     2941 ggatccggaa acaggtactg atgccgaaaa ggcgatcaaa gcctcaggtt ttacggcagt
     3001 atttatcagg acagacattt ctgatccgga ggacattaaa agaatggttg gtgaaactgt
     3061 aagattgttt aataccattg acattcttat aaacaatgca gggatctcag aatgggcgtc
     3121 cccatatgaa ctgcccgttg aggaatggga taaagtaata aacacaaacc tccgtggtac
     3181 ttttctttgt tccagggaag ccgccatggt aatgaaggaa cacggaggag gttccattgt
     3241 gaacattgcc tcgacacgag ctttcatgtc tgaaccagac tccgaagcct atgccgcttc
     3301 taaagggggt atcgttgccc taacccacgc tcttgccgcc tcatttgccg gtgatcatat
     3361 cagggttaac tgtataagcc ccgggtggat tgaaactggt gattataccc gactcaggga
     3421 aactgatcac aaacaacatt attcaggacg ggtaggcaag ccggaagaca tagcaaaagc
     3481 atgcctgtat ctgacagatt atggtaacga ttttataaac gggaccaata tcataattga
     3541 tggcggtatg acacggaaaa tgatatacga atagaagctg aagaaaaagg actataccgt
     3601 caggcccgcc tgaacgaaaa caccgcctgc ccatacggag aaaagccgcc aggaataccg
     3661 gaaccatttg aatcagtgag gccggcatga caaaattttt taaacctagc taaacaaacc
     3721 ctgtagctga attgtttata gctctgatta cagtttcttt taatccggag aaattgcctg
     3781 atggttgaat tcattatata tcataaaatc cagcatcttg ttcgatgaat tacagacgct
     3841 tatgaaataa ttatacctga acgacattgt cttataaatt aaaacaaaat gaaaggcata
     3901 tttccgcttc tgctgcttgt ttccacaatc acattacatg cacaaaaccc ggtgtgttat
     3961 gtttatcacg gctgcggtgg cgccaccgta gctgcctacc cgggagaccc gcaatggaca
     4021 aatataacct atgaatttca gcggtttttc gaaggtaact gggttactgt ccatcagaca
     4081 attgaaaact accacctggt tactcccggt gatatttccc ttgcaaccca ataccgtgca
     4141 ataatgcgca acaatgagac cctccggcag atcatttcaa acagtgtaat tgttgaccct
     4201 gcaagattca atgaagctgt ggccaggccc gaacctaaat taacctttta ctggggaacc
     4261 attgccacaa actggtcaaa ctaccttcgt gtacaaccaa catcattgcc taatctggac
     4321 ggaatgcggc cgccattcac ctttaccatc aaaaagaagg acggttccgt tgtggatcag
     4381 aaattatcaa gcaaccttgt aattttcgac agaaacattg aagcgaatca ggaatatgta
     4441 gttattgtta aggattattg caatcaggtt gactcagtaa ccgggaatat tgcatttgca
     4501 gccaggggcc gtgtagttga cgtaaactgc agcggcgcaa gcgtggaact atacaatgct
     4561 ttcgtcggag agaactttaa cggccggctg cccctgactt atggcctggc gccgcttcat
     4621 gacaccattc tttcaactgc tgtaccccaa agtattattg atggaatcag ctataactac
     4681 tctgaaggac taattacagg cctgacagaa aaacgctatg tgctcagtgc aaaagacagg
     4741 ttcggggtga tttcaagata ttctgtaatc aacaccggta tagactaccc cacggcggcc
     4801 ttgaggggct atggttcata tggcggtttc tgtgaatact atgtaaatct gatgggcgtt
     4861 tatccggagc atggtgtccg gctggccgga accagtgatc catatatctt tactcccgga
     4921 ctgaccgcaa acaatctcag gttagggaaa acatatgaaa ttgtagtacg cgacagctgc
     4981 ggaagattaa gtgagccgac aattatattt ttcccggccg tggatcctgt tatcacctct
     5041 gttgttgttg atacagaaga ttgtaaaaca aaattaaccg tacatgcaca aacctgttcc
     5101 gatacccctg aatatatgct gaaaatctcc cggggtaatc cgggcgtgtg gcagccttca
     5161 aacatttttg tatatgaaag cattacaggt tgtgacacag tttttgtcag agacggtgac
     5221 aaggtaatat ttcaggaaat atgtaccggc actcttgagg cagatgttct tctttatcct
     5281 gtttcagata tctgcggaag tccttatgaa ttgtacgtag gtgccgataa aggctttcct
     5341 ccctacacat ttgaaataag ttctgacggg ataaatttct ctgaccctac agaatatgct
     5401 gtctttaact ggctgccgcc ggcaatatac acggtaagga taactgatgc ctgcgggaat
     5461 acatttgtta ccagccccga agatggagtt gcaggtggct tgttcgaggt aacagaatca
     5521 ggattcatgg gtgaatgtgg cagctcagaa ggatatcttc gcataaatat tgatcccggt
     5581 acagatattc cggtgaatta tccgccctac aaatactctg taaaggagat aatcagcggt
     5641 tcaggataca atatcagtta tggcagtgtt attgccagcg
//
LOCUS       DAOSPX010000209         5669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_12980_length_5669_cov_3.704311, whole genome shotgun sequence.
ACCESSION   DAOSPX010000209 DAOSPX010000000
VERSION     DAOSPX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12980_length_5669_cov_3.704311"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLZ75_09935"
     CDS             complement(<1..573)
                     /locus_tag="PLZ75_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOS72750.1"
                     /translation="MIKEIRILADHRIQYIADLRNQVLSERFRGRPVISAEITSSQAE
                     AAATMCPTAAIDGESGSIDLGRCTFCNACAFMFPGVFRFSADYRIAASERSGLIVKPG
                     QTGWSGFNGLAVRPVIRRLFRRALKLRQVSAGGDNSCEMELHAAGNVNFDMGRYGIEF
                     VASPRHADGIVITGPVSENMADALEMTYRAV"
     gene            complement(592..2088)
                     /locus_tag="PLZ75_09940"
     CDS             complement(592..2088)
                     /locus_tag="PLZ75_09940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOS72751.1"
                     /translation="MNASVPAGTEDIPVLDYSLFREELITMLGREQFHCLNYFCHRIP
                     EGLRFMCCIADDETGTIRILCHEKKDGGTSLRSLSDSCTAMHIFEREIHERYGLGFEG
                     HPWLKPVRYPHDRADRRNVISTYPFYEMESEVLHEVGVGPVHAGVIEPGHFRFICNGE
                     KVLHLEIQLGYQHRGIERLFTEAGENGLRRSLLAESIAGDTATGHGLAHAQLSESLAG
                     FIPPPSLEIERCIALEMERIGIHIGDTAALCADVAYQLGQVACEALRTLIINTTQFWC
                     GNRFGKGLIRPGGSDYPLTEKIADEILRVLGEVERRYVNVTDCVYTLPSVLGRFEETG
                     TVSRQQALSAGAVGMAARSCGLVRDIRASHPFQYYRQMEIKPALQTKGDVMARGRLRV
                     MEVRESAGIIRKLVEVWKNGEKNTGRPVYDLRFMPESLAVSVTEGWRGEICHVALTDA
                     DGRLVDYRIKDPSLHNWMALALAVRNQEISDFPLCNKSFNLSYCGNDL"
     gene            complement(2122..3546)
                     /locus_tag="PLZ75_09945"
     CDS             complement(2122..3546)
                     /locus_tag="PLZ75_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOS72752.1"
                     /translation="MLLFFFIGSVLLSASILLSPGRLVTRVLSLVFSALLIALTVYAW
                     IHQGKTELAYFTFDSTAVLLLSVLALLTVPTLYHGFKYTRRDNLKTYNIYHSALTALI
                     TFMAGAYLANTMTVLWIFVEATTLAVAVLIYHDRTEIALEATWKYIFICSTGIALAYL
                     GILFLGFIYGTGEAPNLSFSSFSLVIAGADPLYLKIAFVFVVVGFSTKMGLFPMHTVT
                     IDAHSVAPPPVSALISTALMNVGFLAIFRIYTLFSTTSILPFMNHVLVLAGVMSLLIA
                     AGYMLKAKHLKRMLAYSSFENMGLVAIALGIGGNGYYAALLLIVLHSLAKSSLFYQMG
                     QLSEILHTFRLDETGRYLQLYPAGGTVLITGMITILAIPPSGMFIPELMIFNTMVING
                     QWFVLVLSLILLTFVVYAMSTRIMHISFSSPRNCRDIRLPGTVNPAETISQFILLGMV
                     IMICFYQPPFLAELIGKSVALLPK"
     gene            complement(3549..4163)
                     /locus_tag="PLZ75_09950"
     CDS             complement(3549..4163)
                     /locus_tag="PLZ75_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72753.1"
                     /translation="MVNYLIVIFAVTLIYFASAERLATYVRLIAVQGLMLCGIAVLEL
                     KEVNLINLLFIVAETLFFKAMILPYLLNRILRRSGVTRVHRQAMPGFYLLLLSIAGLF
                     ISVSLATILVNPFINTIYLAISLFTLYTGLLLIVTHQLILSHLIGFLVVENAVFFFSL
                     AMGSEMPMFINIGLLLDLFVGALILGFFGLKLKPHTNELTMLKD"
     gene            complement(4167..5078)
                     /locus_tag="PLZ75_09955"
     CDS             complement(4167..5078)
                     /locus_tag="PLZ75_09955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOS72754.1"
                     /translation="MAGFLLILVAALFFPGVILRTKSIASGRKGPGIFQPLKDIAVML
                     RKGSVFSDTTGIIFRIAPSVALVSVIVAMLLLPFAQYKALISFEGDFIFFAYLLALGK
                     FFMIIAALDTGSSFQGMGASREALYSMIVEPAFFVLMATFAMFTGYTSFSVIFNHFYI
                     TGNDYVFIYSVIGAYLFIQIAMIENSRLPVDDPKTHLELTMVHEVMILDHSGFDKALI
                     HAGTYLKFAMYGSLIYNLIVPAGWNTPVKVLMFFIVQALFAVVIGITESFRARNKMNK
                     NPKYILTLTSVALIAFMVILILTEKLI"
     gene            complement(5081..>5669)
                     /locus_tag="PLZ75_09960"
     CDS             complement(5081..>5669)
                     /locus_tag="PLZ75_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72755.1"
                     /translation="MLAPQLVILVLILFIGLASPLVVKPVFKLAVSIWDLESQLFIRG
                     TFTYNLSQISLAGGIFIVTLAAVLIWRSFHLSRRITETGPTWGCAYTAVTSRQQYTAT
                     SFASNYSDIAKPVLRTRVQTEIIGESEIFPRKRKFEFHSEDIYKRKLVDRPAGFISDL
                     LKKIAMMQTGQIRHYILYAFVFMLLVLLLSLFNII"
BASE COUNT         1616 a         1407 c         1309 g         1337 t
ORIGIN      
        1 aactgccctg tacgtcattt caagagcatc cgccatattc tcactgacag gtccggttat
       61 tacaatgccg tcggcatgac gcggtgaggc aacaaactca ataccgtaac ggcccatgtc
      121 aaaattcacg ttcccggctg catggagttc catttcacag gaattatccc ctcctgccga
      181 aacctgcctg agtttgagag ctctcctgaa aagcctgcgg atcacgggtc tgacagccaa
      241 tccattgaag ccggaccagc cggtctgacc gggctttact atcagaccac tcctttctga
      301 tgccgcaatc cggtagtcag cggaaaagcg gaaaaccccg gggaacatga aggcacatgc
      361 attgcagaaa gtacaacggc caaggtcaat tgaaccgctt tccccgtcaa tggcagcagt
      421 gggacacatc gtagcggcag cttcagcctg ggatgatgta atttcagcag atatgacagg
      481 ccttcccctg aaacgttctg aaaggacctg atttcgcaga tcagctatgt actgtatgcg
      541 atggtcagcc aatatcctga tttcctttat cataattttc cattctgccc attacagatc
      601 gttgccgcaa taggataagt taaaactctt gttgcacagg ggaaagtccg atatttcctg
      661 attgcggacc gcaagtgcca gggccatcca gttgtgcagt gagggatcct tgatcctgta
      721 atccaccagc cggccatcag catcagtcag ggccacatgg catatttccc ccctccatcc
      781 ttcggtaaca gacactgcaa ggctctccgg catgaatctg agatcataaa caggccggcc
      841 cgtattcttt tccccgtttt tccatacttc aaccagttta cggatgatcc ctgccgattc
      901 tcttacttcc atgacgcgca acctgccacg ggccatgaca tcaccttttg tctgcaacgc
      961 aggttttatc tccatctgtc tgtaatactg gaaggggtgg cttgcacgta tatcccggac
     1021 aaggccgcat gaacgggcag ccattcccac ggcccctgct gaaagagcct gctgcctgct
     1081 tacagtacct gtttcctcaa agcggcccag cacactgggc agcgtgtaaa cacaatctgt
     1141 cacgttgaca tatcttcttt caacctcccc gagtaccctg agtatttcat ccgcaatctt
     1201 ttccgtaaga ggataatccg atccgccggg acggatcagt cccttcccga aacgattccc
     1261 gcaccagaat tgtgtggtat tgataatcag cgtccgcaat gcctcgcatg caacctgccc
     1321 gagctgatag gccacatctg cacacaatgc cgcagtatca cctatgtgta tcccgatacg
     1381 ttccatttca agggcaatac atctttcgat ctccagtgaa ggaggcggga taaatcctgc
     1441 cagcgactcg gaaagctgcg catgggccag tccgtgacct gtggcagtgt caccggcaat
     1501 actttcagcg agcagggaac gccggaggcc attctccccc gcttccgtaa aaagccgctc
     1561 aatcccccga tgctgataac ccagctgtat ctccagatgc aggactttct caccattgca
     1621 gataaacctg aaatgaccgg gctcaatcac gccggcatga acaggtccta ctccaacctc
     1681 atgaagcacc tcgctttcca tttcataaaa cggataagtg cttatcacat tccgcctgtc
     1741 ggccctgtca tgaggatatc tcacgggctt aagccaggga tggccttcaa agcccaaacc
     1801 gtatcgttca tgtatttcac gttcaaatat atgcatggcc gtacatgaat cagaaagtga
     1861 tctcagggat gtcccgccgt ccttcttctc atgacagagt attctgatgg taccggtttc
     1921 atcatccgca atacagcaca taaaacgcaa tccttcaggg atcctgtggc agaaataatt
     1981 caggcaatgg aattgttccc tgcccagcat ggtgataagc tcttcacgaa agagggaata
     2041 atcaagtacc ggaatgtctt ctgtgccggc aggtacagag gcattcatga cttctgtata
     2101 tcttaaactg gtcatctttt tttactttgg gagcaaggca acacttttgc ctatcagttc
     2161 agccagaaag ggcggctggt aaaaacaaat cattataacc atacccagga ggatgaactg
     2221 gctgatcgtt tctgccggat tcacagtacc gggcagtctg atatccctgc aattccttgg
     2281 tgatgagaaa ctaatatgca ttattcttgt actcatggca taaaccacaa atgtgagaag
     2341 tatgagcgaa agcaccagaa caaaccactg gccgttaatg accatggtat tgaagatcat
     2401 cagctccgga ataaacattc cggaaggggg aatggccagt atggtgatca ttccggttat
     2461 cagaacagtt ccgcctgcgg gatagagttg taaatacctt cctgtttcat cgagcctgaa
     2521 agtatgcagt atctcggaaa gctgccccat ctggtaaaaa agactggatt ttgcaagtga
     2581 atggagcaca ataagcagca aggcggcata atatccatta ccgccaatac caagggcaat
     2641 cgccaccagt cccatgttct caaaactgga ataggccagc atacgcttca ggtgctttgc
     2701 cttgagcatg tatcccgcag caataagaag agacataact cctgccagaa ccagcacatg
     2761 attcatgaaa ggcagaatag acgtggtgga gaagagggta taaatacgga aaatagccag
     2821 gaatcccaca ttcatgagag cggtggaaat aagtgcactc acaggcggag gagccaccga
     2881 atgggcatct atggtaacgg tatgcatcgg gaaaagtccc atctttgtgc tgaatcccac
     2941 aaccacgaat acgaaggcaa tcttcaggta aagcggatca gctccggcaa tcacaaggga
     3001 gaatgaggaa aatgagaggt tgggtgcctc accggttcca tagataaatc cgaggaaaag
     3061 aattcccaga taggccaggg ctatcccggt ggagcagatg aatatatatt tccatgtggc
     3121 ttcaagtgct atttctgtac ggtcgtgata aatcagcacc gccacggcaa gggtggtggc
     3181 ttccacgaaa atccagagga cagtcatggt gtttgcaagg taggcacctg ccataaaggt
     3241 tataagtgcc gtcagagctg aatggtatat gttgtaagtt ttcaggttgt ccctgcgggt
     3301 gtatttgaat ccgtgataca atgtcggcac ggtaaggagt gccagtaccg aaagcagaag
     3361 tactgcagta ctgtcgaaag taaagtaggc aagttctgtt ttgccctgat gtatccatgc
     3421 atacacggta agggcaatca gcaatgcaga aaagacaagt gaaagcaccc tggtaacaag
     3481 cctgccggga gacaggagta ttgaagcact cagcaatact gaccctatga aaaaaaacag
     3541 cagcatcttt aatctttcaa cattgttagt tcatttgtat gaggtttcag cttcagtccg
     3601 aagaaaccca gtataagggc cccgacaaac aggtcaagca acaggccgat gtttataaac
     3661 atgggcattt cgcttcccat cgccagggaa aagaaaaaaa cagcattttc aacaaccagg
     3721 aatcctatca gatgtgaaag aataagctga tgtgtcacta tcaggagaag acccgtataa
     3781 agcgtaaaga gagaaatagc caggtaaatg gtgttaatga agggattcac aagaattgtt
     3841 gccagactga cactgataaa caggccggca atagaaagca ggagaagata aaaaccgggc
     3901 attgcctggc ggtgaaccct ggtcacccct gaccgcctaa gtattctgtt gaggaggtaa
     3961 ggcagaatca ttgctttgaa aaagagtgtc tctgccacaa taaaaagcag gttgatcaga
     4021 ttcacctcct tcagctccag caccgcaatg ccgcataaca tcagaccctg aacggcaatc
     4081 agcctgacat aggtggccag tctctccgcc gatgcaaaat aaatcagggt gactgcaaaa
     4141 ataacgataa ggtaatttac catttttcag ataagttttt cagtcagtat cagtattacc
     4201 atgaacgcaa taagtgcaac cgaagtcagt gtcagtatat atttcgggtt cttgttcatt
     4261 ttgtttcgtg ccctgaatga ttctgtaatg ccgatcacaa cggcaaacag tgcctggacg
     4321 ataaagaaca tcagaacctt caccggggta ttccacccgg ccggcactat cagattatat
     4381 atcagcgaac catacatggc aaatttcaga tatgtgccgg catgaatgag agccttgtca
     4441 aagccgctgt ggtcaagtat cataacttca tgaaccatgg tgagctcaag atgggttttc
     4501 ggatcgtcaa ccggaagcct gctgttctcg atcatggcta tctgtataaa cagataggct
     4561 ccgataacgc tgtatataaa gacataatca ttccctgtaa tatagaaatg attgaatatt
     4621 acggaaaagg atgtatatcc ggtaaacatg gcgaatgtag ccatcagcac gaaaaaagcc
     4681 ggttccacaa tcatggagta aagggcctcg cggcttgccc ccatcccctg aaaactgctt
     4741 cccgtatcaa gagcggcgat aatcatgaaa aacttaccaa gggccagaag gtaggcaaaa
     4801 aaaataaaat ccccctcaaa agagatcagt gccttgtatt gggcaaaggg cagcagaagc
     4861 attgcaacaa taaccgacac aagagcaacg gatggcgcta tccggaatat gatacccgtt
     4921 gtgtcgctga aaacacttcc ctttctcagc ataaccgcta tgtctttcag gggctgaaat
     4981 attccgggac ctttccggcc cgaggcaatg ctttttgtcc ggaggattac tcccggaaaa
     5041 aacagggctg caaccaatat cagtagaaaa cctgccatgc tcaaataatg ttaaacaatg
     5101 aaagcaacaa taccagcagc atgaaaacaa aggcatacag aatgtaatgc ctgatctggc
     5161 cggtctgcat catcgcaatt tttttcagca ggtcagaaat aaagcctgcc ggtctgtcaa
     5221 ccagcttcct tttataaata tcctcgctgt gaaattcaaa tttccttttc cgggggaaaa
     5281 tctcgctctc gcctattatt tcagtttgca ctctggtgcg cagcaccggt tttgcaatat
     5341 cgctatagtt ggatgcaaat gatgttgccg tgtattgctg ccttgaagtc acggcggtat
     5401 atgcgcaccc ccaggtcggg cccgtttcag taatccttct gctcaggtga aaactgcgcc
     5461 atatgagcac tgccgcaagt gtgacaatga aaattcctcc tgccagactg atctgtgaca
     5521 ggttgtatgt aaaggttccg cggataaaca actggctttc caggtcccat atactgactg
     5581 ccagtttaaa aaccggtttc accactaacg gtgatgccag tccgataaac aatatcagta
     5641 caaggataac aagctggggg gcgagcatc
//
LOCUS       DAOSPX010000210         5623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13114_length_5623_cov_5.406250, whole genome shotgun sequence.
ACCESSION   DAOSPX010000210 DAOSPX010000000
VERSION     DAOSPX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13114_length_5623_cov_5.406250"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLZ75_09965"
     CDS             complement(<1..296)
                     /locus_tag="PLZ75_09965"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOS72756.1"
                     /translation="MNKGVKEFSKTKLRSSYVTLVVSVSLVLFLLGILGLVLINAKGL
                     ADYFRESLSFWVMLDDDAKEADIRLLQKDLDAKDYVKSTEYVSKEKAAAKMKEEL"
     gene            complement(394..1413)
                     /locus_tag="PLZ75_09970"
     CDS             complement(394..1413)
                     /locus_tag="PLZ75_09970"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOS72757.1"
                     /translation="MEKLDWKNLHFGYMKTAFNVRSYYRDGKWSKPEVSDSEYLNIHI
                     AATGLHYGQQAFEGLKAYMGKDGKIRLFRWQENARRLILSAEGIKMATVPEELFREAI
                     FRAILENRQFVPPYGTGATLYIRPLLFGSGAEVGVKPASEYVFVVFVTPVGPYFREGI
                     KPVSMMICRNVDRAAPLGTGTFKVGGNYAASLRALLAANEGGYSSAIFLDAREKKYID
                     ECGPANFYGIKGDKYITPKSNSILNSITNMSLIDIASSMGMKTERRPIPVGELSTFSE
                     AGACGTAAVITPIARIVDPEKNMVYEYCSDGKPGPVTMELYNRLVAVQNGDAPDEFGW
                     ITLVE"
     gene            complement(1471..3327)
                     /locus_tag="PLZ75_09975"
     CDS             complement(1471..3327)
                     /locus_tag="PLZ75_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOS72758.1"
                     /translation="MSFCRSLKAILIISLIIPAVFTDGPAMAPENIPVLKEIILNSDS
                     VIGIPPDIREMPQITVSKKYNNIGFELEKADSLVYMFLLEGFDRQWSQWSLHGFKEYT
                     NLPAGKYSFRVRYTDLETTGGEEHLADLRVLPVWYLSFPALLMYAILAALLVWTFYSF
                     LNLRFARTQYMLERIISKRTEDLIVEKEKTENLLANVLPRNTASEIMETGKAAKVKYN
                     FVTVLFSDIQGFTKLAEEMNPELLIDELDKFFFYFDSVVEKYGIEKIKTIGDAYMCAG
                     GIPEKNRTNPVEVILAALEMQDYMSRLKETSRIGGMELWDIRIGIHTGTVVAGVVGQK
                     KLSYDIWGDTVNTASRMESSGEAGKINISGTTREFVKDFFECEYRGKMPVKYKGEIDM
                     YFVNGIVPGLRDENGKPNGRFWLKMRMIKLLDIEEAVIKMYDDEAAPNLYFHNSQMVR
                     NISNQAELLSRAENLPDEEFLRVRLAALFLFTGFISDSGYDNHAESSVLMVEELLPAH
                     GFERKDIDGIKRLIRNSFEDIRESHSDNILHDARYDYLGRIDCIMLTDRLLKEEAEYG
                     KIYSKEEWLDIQLKFLADHEFRTEKARILRSVSSEEQAAALRDFYSPAGLLR"
     gene            3433..4035
                     /gene="rdgB"
                     /locus_tag="PLZ75_09980"
     CDS             3433..4035
                     /gene="rdgB"
                     /locus_tag="PLZ75_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOS72759.1"
                     /translation="MDIIFATNNENKTREIRNILGSNINLLSLNDLNIHEDIPETEPT
                     LEGNALQKARYIYEKVSRCVFADDTGLEIDMLDGLPGVNSARFAGENKDSEANIDKVL
                     LMMADSERRSARFRTVIALVEGGKEYLFEGIVEGRITGERRGTGGFGYDPVFIPRGET
                     RTFAEMPLDEKNRISHRAMAFEKLKTFLDNQSCCPEEDAV"
     gene            4051..>5623
                     /locus_tag="PLZ75_09985"
     CDS             4051..>5623
                     /locus_tag="PLZ75_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72760.1"
                     /translation="MRKTLMLTYLILISAGLFSQTPVGTWSDHLVYTRTGSLALSPEE
                     VFASTGSSLLVYDRKYDELRKLTRINGLTETDISTIAWSEESRTLVIAYLSTGLDLIR
                     DNAIYHIPDIKNKYIPGKKTINRIRTRGKYAYLACSFGIVVVDLIKREIFDTWKPGDI
                     FRTGEVRDISFGDDGKIFAATDIGLYYAAISSQGLSYPGNWSPENRLPYPGGNYNAVF
                     FSDGRLYANRTGEYFDGDSLYVIGSSVTLFSYEPGVFNESIEPSSGGFTVSSSGMARF
                     YSPGGQLIKMINSYPWGLPDISMAAADRDLWIADKSSGLVCFRNMNEPVSLTLPGPFS
                     NEAVSISSHGGKTIVCGGGVDASWNNLWKPFSLSVFENNSWKLITSIPNNDAMRVVFN
                     PSDRNHFFVSTWGTGLLEYRNNELVNSYTDLNSPLQNIIPGKPYVRICGMAFDRRGNL
                     WLAQPEVPNTIKALKPDGTWIVNPASANVYTVGDLVITRTGHKWLLLPRGFGLFLLDD
                     NDTPEIFNDDVHKKIL"
BASE COUNT         1532 a         1380 c         1200 g         1511 t
ORIGIN      
        1 agctcctcct tcatttttgc agcagccttt tcctttgaaa catattccgt cgatttcaca
       61 tagtccttcg catcaagatc cttctgaagc aggcgtatgt cggcttcctt cgcatcatca
      121 tcgagcatca cccagaagga gaggctttca cggaaataat ctgcaagtcc ttttgcattt
      181 ataagtacaa gacccagtat gcccaggaga aaaagaacaa gtgacacact aacaacaagg
      241 gtcacatatg aacttctcag ttttgttttc gaaaattcct ttactccctt attcataaat
      301 tatcagaatc agcctgtttt taaaatcatg aacgccactg cgcggataat ttctggcaaa
      361 tggcgacgca atctgaaata ttttttcaac cggctattcc accagggtga tccatccgaa
      421 ttcatcggga gcgtcaccat tctgaaccgc gacaagcctg ttgtataatt ccatcgttac
      481 cggaccgggc ttcccgtcac tgcagtattc ataaaccata ttcttttcag gatcaacaat
      541 cctggcaatg ggcgttatca ctgctgccgt cccgcaggca ccggcttcag aaaatgttga
      601 caattcgcca accggaatgg gtcttcgttc tgtcttcatc cccatggatg aggcgatatc
      661 gataagactc atgttggtta tggaattcag aatggaattt gatttcggag taatgtactt
      721 atctcccttt atcccgtaaa aatttgccgg accgcattca tcaatatatt ttttctccct
      781 ggcatcaagg aagatggcac tggaatatcc gccttcattt gcggccagca gtgcacgcag
      841 gcttgcggca tagttccctc ccaccttgaa ggtacctgtt cctagcggtg cggccctgtc
      901 tacattcctg catatcatca tgctgaccgg ctttatgcct tccctgaaat agggaccgac
      961 cggtgttaca aacacaacga aaacatattc cgacgcgggt tttacaccca cctcagcccc
     1021 gctgccaaac aacaggggcc ttatgtagag ggttgccccg gtaccgtaag ggggaacaaa
     1081 ctgcctgttt tccaggattg cacggaaaat ggcttcccgg aacaattcct cgggaacggt
     1141 ggccatttta ataccttcgg cagacaatat cagtctcctg gcattttcct gccagcggaa
     1201 aagccttatt ttcccgtctt tgcccatgta tgccttcagc ccttcaaagg cctgctggcc
     1261 gtaatgcagg cccgtggccg caatgtgaat attaaggtat tcggaatcag acacctcagg
     1321 tttgctccat ttgccatccc tgtaatagga acgaacatta aaggcagttt tcatataacc
     1381 gaaatgcaaa ttcttccagt caagtttttc cattgcagat ccttttaatt aagccgtaaa
     1441 agtacattat aaaaatctat tttcggattt ttatcttaat aatccggccg gggaataaaa
     1501 atccctgagt gcggctgcct gctcttcaga ggatacagaa cggagaatcc ttgccttttc
     1561 agtcctgaat tcatgatcag caagaaactt cagctgtata tccagccatt cctccttact
     1621 gtaaattttc ccgtactcag cctcttcctt cagaagcctg tccgttaaca ttatgcagtc
     1681 aatccttcca aggtaatcat agcgggcatc atgcagaata ttatcagaat gcgattcccg
     1741 gatatcctca aaggaattcc ttataagccg tttaattccg tcaatatctt tccgttcaaa
     1801 tccatgggcc ggcagcagtt cctcaaccat cagcaccgag gattcagcat gattgtcata
     1861 gccggaatca gaaatgaagc ccgtgaacaa aaacagggct gcaagcctga ctctcaggaa
     1921 ttcctcgtca ggaagatttt ccgcccttga aagcagttct gcctgattgc ttatattcct
     1981 gaccatttgc gaattatgaa aatacaggtt cggagcagct tcatcatcat acatcttgat
     2041 aactgcctct tcaatatcaa gaagtttgat catccgcatt ttcagccaga atctcccgtt
     2101 tggtttcccg ttttcatccc tcagcccggg cacaattccg tttacgaaat acatatcgat
     2161 ctcgcccttg tattttaccg gcatcttacc ccggtattca cattcgaaaa agtcttttac
     2221 aaactccctg gtagttcccg aaatattgat ttttcccgcc tcccccgagg attccatcct
     2281 gcttgccgta ttcacggtat caccccagat gtcataggaa agttttttct gtcccaccac
     2341 tcccgctacc accgtacccg tgtgaatccc tattctgata tcccataatt ccattccgcc
     2401 aatcctcgaa gtttccttca gtctcgacat ataatcctgc atttcaaggg ctgccagaat
     2461 aacttcaaca ggattggtcc tgttcttttc gggaatgcct cccgcacaca tgtatgcatc
     2521 ccctatggtt ttaatctttt caatgccgta tttctccaca acagaatcaa aatagaaaaa
     2581 gaatttgtca agctcatcaa tcaggagttc cggattcatt tcctcagcaa gcttcgtgaa
     2641 cccctgaata tccgaaaaaa gcaccgtgac aaaattatat ttcaccttgg cagccttgcc
     2701 tgtttccatt atttcgcttg ccgtgttcct gggcaggaca ttcgcaagca ggttttctgt
     2761 tttctccttt tcaactatca ggtcttcagt tcttttactg attatccgtt caagcatgta
     2821 ctgggtccgc gcaaagcgca ggttcaggaa cgaataaaat gtccagacca gcagggcggc
     2881 cagtatcgca tacatcagaa gagccgggaa agacaggtac catacaggca gtacccggag
     2941 atctgccaga tgttcctctc cccctgtggt ttccaggtcg gtgtatctca ccctgaaact
     3001 gtattttccg gcaggcaggt ttgtatattc cttaaaaccg tgaagcgacc actgactcca
     3061 ttgcctgtca aagccttcaa gcaggaacat gtaaacaagg gaatcagctt tctcaagttc
     3121 aaatccgata ttgttatatt ttttactgac agttatctgc ggcatttccc ttatatccgg
     3181 cggaattccg ataacagaat cactattgag aattatctcc ttcaataccg gaatattttc
     3241 aggggccatt gcaggaccat ctgtgaaaac agccggaata atcagtgaaa taatcagaat
     3301 tgctttcaga cttctgcaga aactcataca aattaccgga atatatcaat tcaaatgtac
     3361 tacttaatca tattcagtca aaaaggaaaa ccttattttc agtatttttg atcccggatt
     3421 aactgagctt ttatggatat catatttgcc acaaacaatg aaaacaagac ccgggaaatc
     3481 agaaatatcc ttggcagtaa tattaacctc cttagtctta atgatttaaa catccatgag
     3541 gacattccgg aaaccgaacc aaccctcgaa ggaaatgctc ttcagaaagc ccgttatata
     3601 tatgaaaaag tcagccgctg tgtctttgct gatgacacag gactggaaat tgatatgctt
     3661 gacggcctgc ccggagtaaa ttccgcacgt tttgcgggag aaaacaagga ttctgaggca
     3721 aatattgaca aggtgctcct catgatggct gattcggaaa gaagaagtgc caggttcagg
     3781 acagtgattg cacttgtcga aggaggaaaa gaatatctgt ttgaagggat tgttgaaggc
     3841 aggatcacag gagaaagaag ggggacagga ggattcggat atgatccggt gttcattccc
     3901 cggggtgaaa ccaggacctt tgcggaaatg ccccttgatg aaaagaacag gatctcgcac
     3961 agggccatgg cttttgagaa gttaaaaacc tttcttgaca atcagtcctg ctgcccggag
     4021 gaggacgctg tataaaggaa cacacgcctg atgagaaaaa cattgatgct gacatacctg
     4081 atcctcattt ctgcaggctt attctcccag acacccgtgg gaacatggag cgatcacctt
     4141 gtttacacca gaacaggcag cctggcactc agtcctgaag aagtatttgc ctcaacagga
     4201 tcatcattgc tggtgtatga caggaaatat gacgagctgc gcaaattaac caggataaac
     4261 ggtctcactg aaacggatat cagcacgatt gcctggtcgg aagaaagcag gactctggta
     4321 attgcctatt taagtaccgg actggatctt atccgggaca atgccatcta ccatatccct
     4381 gatataaaaa acaaatacat tccgggaaag aaaactatca acagaataag gaccagggga
     4441 aaatacgcat acctggcctg cagtttcggc attgtggttg tcgatctcat aaagagggaa
     4501 atatttgaca cctggaagcc gggggatatt ttccgcacgg gagaagtcag ggatatatcc
     4561 ttcggggatg acggaaaaat tttcgctgca acagatattg gattgtatta tgcagccatt
     4621 tcttcccagg gattgtcata tccgggaaac tggtcgcccg agaacaggct cccctatccc
     4681 ggaggaaact acaatgctgt ttttttctct gacggcaggc tctatgccaa caggacggga
     4741 gagtattttg acggcgactc actgtatgta atcgggtcat cagtcacatt attctcatat
     4801 gaacccggtg tgttcaacga atcaattgaa ccctcgtcag ggggattcac ggtttcgtcg
     4861 tccggaatgg cacgcttcta ttctccgggc gggcagctga tcaaaatgat aaactcctat
     4921 ccctggggtt tgcctgacat ctcaatggct gctgccgaca gggatctgtg gatagcagac
     4981 aagtcatccg gactggtctg tttcaggaac atgaatgaac ctgtatccct gacacttccg
     5041 ggtcctttct ccaatgaagc agtcagtatc agttcacacg gcgggaaaac gattgtttgc
     5101 ggaggagggg tggatgcatc atggaacaac ctctggaaac cattcagcct gtcagttttt
     5161 gaaaataatt cctggaagct gatcacatcc attccgaaca atgatgccat gagggtcgtt
     5221 tttaatcctt ccgacaggaa tcatttcttt gtatccacgt ggggaacggg actgcttgag
     5281 taccggaaca atgagctggt taacagttac accgacctca attcgcccct gcagaatata
     5341 ataccgggaa aaccctatgt gagaatatgc ggaatggcat ttgacaggag gggtaatcta
     5401 tggcttgcac agccggaggt tccaaataca ataaaggcac tgaagcccga cgggacgtgg
     5461 atagtaaatc cggcatcagc caatgtctat acagtgggtg acctggtcat taccaggaca
     5521 ggacacaaat ggctgctgct tccccggggg ttcggtctct tccttctgga tgacaatgac
     5581 acgcctgaaa tattcaatga tgatgttcac aaaaagatcc tgg
//
LOCUS       DAOSPX010000211         5618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13133_length_5618_cov_3.900773, whole genome shotgun sequence.
ACCESSION   DAOSPX010000211 DAOSPX010000000
VERSION     DAOSPX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13133_length_5618_cov_3.900773"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="PLZ75_09990"
     CDS             <1..1051
                     /locus_tag="PLZ75_09990"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOS72761.1"
                     /translation="IPVLLILPLPVVLFYSLIPERHLEKTSFLIIQLIVIPAMALTGF
                     FKMVNFDAERTMTYDQLVRNRKWEKVIAFAGKKPPGNYLSLAMLNLSLAKTGIMAEEM
                     FSFSQHGREGLFLRFEDEFISPMMGNEILYHIGLVNASQLYAFESMEVMPHMEKSVRA
                     VKRLAETNLINGRYEVAGKYLKLLEKTLFYRKWALNTGKYLYNEEMINNHPDWGEKRK
                     MAVRKDFFFHIDDIEAILIRMLSENPGNRIAFEYLAAWYLLGRELDKFAGLVPLMQQL
                     GYREMPRSCQEAMLFYISLTNTDPLAGSPYRISPDVIQRMNSYARIYLNNPDARHLLA
                     PAYSDTYWYYFHYAE"
     gene            complement(1058..1756)
                     /locus_tag="PLZ75_09995"
     CDS             complement(1058..1756)
                     /locus_tag="PLZ75_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOS72762.1"
                     /translation="MRSYIFLFLLVFTLAPLTLQGQKSSRKITLSGRVADKDSQPVKG
                     AVIFIDKVKTDVVTDDQGKYRIRVSPAASEILVFSLFSGVSSQNIDGRTVIDFILTGE
                     TRDVPGNDSGNKDAMAAGDRNREKDKEEGRLVFDPKDARHSSYQSIYDMIRGRFPGVE
                     VSGNNIRIMGSSSFHVSTEPLFVVDGVIVNSIDNISPQTVRSIEVLKGPDATVYGTRG
                     SNGVIVITRISGKD"
     gene            complement(1843..2829)
                     /locus_tag="PLZ75_10000"
     CDS             complement(1843..2829)
                     /locus_tag="PLZ75_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS72763.1"
                     /translation="MKKKTISRRRFLGTAATAAAAFTAIPVISSCSSGPGQKKPNSKV
                     GGVQLGCTTYSYRSMPHKVDDVIEYLLFAGINSIELRLVAEEDLGIPAMPQRPRVQEM
                     TDELRAEFARQMEEVREAQRQWRLSLPMSRYEDMRKKFNNAGIDVYISKFAPSSWSDE
                     EIDYAYTAAKTLGSIGITDEYSETAIHRLGKFAEKHGSLAMFHTHGQPAEPGFSFDPI
                     FAASPANYLNLDAGHYFGATGLHPNEVIIKYHDRMRSIHIKDKTGPDSTPAGANMPFG
                     QGETPIADILLLLKKEKWPIQVDIELEYQIPDDSDAAREVAKCIEYMRNILE"
     gene            complement(3184..4914)
                     /locus_tag="PLZ75_10005"
     CDS             complement(3184..4914)
                     /locus_tag="PLZ75_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS72764.1"
                     /translation="MKRYIVIISSKCTLLAGILLLMLSSCNDKEILKEVPKDFLAPEN
                     AYSTVSGIRQGISGLHYTFRNYWFWGDQDQGSMWRGVGTDVAFHGEDPNSTRFLCNYV
                     NYLIPGSAYVLQYWTRNYELIQRANVLIEKVEASDAQIWSGEAQRNAFKAEAMFFRAY
                     AYRILVSYFGDVPLVTEAIKSAKADFTRTPVADVYKQIEDDLKYAAANLPGRGKEEAP
                     GRITQAAAWHLLSEVYLNQGKYQLAVDAASEVIDGGEYGLMTERFGAQHDVFGSGDVY
                     YDLFRYGNQNLSENKETIWAIQFEPLITGGASWSGERGFGPAYFRWGNAPDGKAAFRG
                     EFVNGKYTGYSDTLGRPVSWVRPTDYAAYTIWRRSGWNEDIRNAKHSIKRKFYFDNPE
                     SAFHGQELKFALFNAEYAGNKRDAIRDSCQYIYPYFMKFADPCNHFDNPGQSGGGYTH
                     QDVYAMRVAETILNRAEGYLGLGKKDLAAADINRIRSRAQAPPVDEADVNIEYILDER
                     ARELYGEEWRHIVLRRVGKLVERVRQYNNNPKNPGLHIQDHHVLWPIPLSQIDLNIDA
                     ATGQNPGYPK"
     gene            complement(4953..>5618)
                     /locus_tag="PLZ75_10010"
     CDS             complement(4953..>5618)
                     /locus_tag="PLZ75_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS72765.1"
                     /translation="GNAWFVGEPIQAIYDYEMAGGLWTEADLYSGNILNNWYPGQYKY
                     VDQDKNGVIEPTNDRKIVGYKSPSYRFSINNTLNYKNFTLSFFINSIQGGKKYYMLDN
                     ASVVNVAWRSDDVLRINASAVRPYWTPDNGVNNATGVYNSPAVSSGIYESRSFVRLQD
                     VSLSYNFSSQLLNTLKLQSCTFYIASKNPYVWTKWSGWDPETGTSNNPLMRNITAGFN
                     VTF"
BASE COUNT         1423 a         1369 c         1262 g         1564 t
ORIGIN      
        1 cataccggtt ctccttatat tgcccctccc cgttgtttta ttttattcac tcattccgga
       61 aagacatctg gaaaaaacat ccttcctgat tattcagctt atagtaattc cggctatggc
      121 cttaaccgga ttttttaaaa tggtgaattt cgacgcggaa cgaacaatga cttacgatca
      181 gctcgtcagg aaccggaaat gggaaaaggt aatcgcattt gcggggaaaa agcctccggg
      241 gaattatctg tcgcttgcca tgctcaacct gtcacttgcc aaaacgggta tcatggctga
      301 ggaaatgttc agtttcagtc agcatggcag agaaggtctt ttcctgagat ttgaggatga
      361 atttatcagt cccatgatgg gaaatgagat cctttatcac atcggactgg taaatgcatc
      421 acagctctat gcctttgaga gcatggaagt gatgccccac atggaaaaaa gcgtgagggc
      481 cgtaaagaga ctggcggaaa ccaatctgat aaacggccgg tacgaggtgg caggtaaata
      541 tctgaagctg cttgagaaga cactatttta cagaaaatgg gccttaaata ccgggaaata
      601 tctttacaat gaggagatga tcaataatca tcccgactgg ggtgaaaaaa gaaaaatggc
      661 agtcaggaag gatttctttt ttcatattga tgatattgaa gccattctta tcagaatgct
      721 gagtgagaat cccggaaacc ggattgcatt tgaatatctt gcagcatggt atctgctcgg
      781 aagagaactt gacaagttcg ccggactggt tcctctgatg caacagctcg gttacaggga
      841 aatgccacgg tcctgccagg aagcaatgct gttctatatc agtcttacca acacggatcc
      901 cctcgcagga agtccctaca gaataagtcc ggatgtgata cagaggatga attcctatgc
      961 aaggatctat cttaacaatc ctgatgcccg gcatctcctg gcaccggctt actcagacac
     1021 atactggtat tattttcact acgccgaata acaggagtca gtccttgccg cttatacgcg
     1081 ttatcactat cactccgttg gatccgcgtg tgccgtaaac agtggcatcc ggtcctttaa
     1141 gaacctctat tgatctgacg gtctggggtg atatgttatc gatggaatta acaatgactc
     1201 catcaacaac aaagagaggt tccgtactca cgtgaaaaga agaggatccc atgattctta
     1261 tattgtttcc gctcacttcc acgcccggaa acctcccccg gatcatgtca tagatgctct
     1321 gatagctcga atgcctggca tctttgggat caaaaacaag gcggccttcc tctttgtctt
     1381 tttccctgtt cctgtctccg gctgccatgg catctttatt accggaatca ttcccgggca
     1441 catcacgggt ttcgccggta agaataaagt ctattacagt tcttccgtca atattctgtg
     1501 aggatactcc gctgaaaaga gaaaagacga gaatttcgga ggcagcagga cttaccctta
     1561 tcctgtactt accctgatcg tctgtgacaa catcggtctt gaccttgtca ataaagatca
     1621 ctgcaccctt aacaggctga ctgtccttat cggccaccct gccgctgagg gtgatcttcc
     1681 tgctgctttt ctgcccttga agagtcagcg gtgcaagagt gaaaacaagc aggaaaagaa
     1741 atatatatga tctcatttac atgatatttg aattcagggc aggaacggca gccctccgcc
     1801 tttccccgga aaagctgccg ttacatgtat gtcagcaaat gcttactcga ggatgttcct
     1861 catgtattcg atgcatttcg caacttcccg ggcagcatcg gaatcatcgg gtatctggta
     1921 ttcaagttca atatcaacct gtatcggcca tttctctttc ttaaggagca ggagtatgtc
     1981 ggctatgggt gtctcaccct ggccgaaggg catgtttgca cctgcaggag tgctgtccgg
     2041 accggtcttg tcctttatat ggatactgcg catcctgtcg tgatacttta taatcacttc
     2101 attcggatga aggcctgttg ctccgaaata gtgtcctgca tccaggttga gatagttggc
     2161 aggtgatgca gcaaatatag gatcaaatga gaatcctggt tctgccggct ggccatgggt
     2221 atgaaacatt gccaggctgc catgtttttc tgcgaatttg cccaggcggt ggatggccgt
     2281 ttcgctgtac tcatccgtaa taccgattga accgagggtt ttggctgccg tgtaggcata
     2341 atcaatctct tcatccgacc atgaagatgg agcaaatttc gatatgtaca catctattcc
     2401 ggcattattg aactttttgc gcatatcctc atatctcgac attgggagtg aaaggcgcca
     2461 ctggcgctga gcttccctta cctcttccat ctgtctcgca aactcagccc tcagttcgtc
     2521 ggtcatttcc tgaaccctcg gcctctgggg catggccggt ataccaagat cctcctcggc
     2581 taccagtctg agttcaattg aattgatccc ggcaaagaga aggtattcaa taacatcatc
     2641 caccttatgc ggcatgctcc ggtagctgta ggtcgtacag ccaagctgca cccctcctac
     2701 ctttgaattt ggttttttct gacccgggcc tgatgaacat gaactgatca caggtatggc
     2761 agtgaaagca gctgcagccg tggcagcagt tccaaggaag cgtcgcctgc tgattgtttt
     2821 tttcttcata ctgttaattc ttttttaggg ttattaaggt attgtcattc ttattcgcta
     2881 attgataaaa ttaagaattc tgaccgtgta ttcaaacact attaattcat ttaacaatta
     2941 ttagtatttg acggcctccg gaatcgttac tgcctgcagg gatctttacc gggataacgt
     3001 gcggtgcaga ttatcaccgg gttctgtgca gggcaaatct tcaccagggg taccaaacag
     3061 cacaaactac ctctgggata ctgagcggta caaatcatcc ccggaataca gagcggtaca
     3121 aatcatcagt gggcaaagca gctttgccca ctgatattaa tttttatacc ggtaagggtg
     3181 tgactatttc ggatatcccg gattctgccc ggttgccgcg tcaatgttca ggtcgatctg
     3241 cgacaggggt atcggccaca gtacatggtg atcctgaata tgaaggcccg gattcttggg
     3301 gttattgttg tactgcctga cccgttcaac aagcttcccg acccggcgca aaacaatatg
     3361 tctccattcc tccccgtaca gttccctggc acgttcatcg agtatatact caatattcac
     3421 atccgcttcg tccaccggcg gtgcctgtgc cctgctcctt atcctgttga tatcggcagc
     3481 tgcaagatcc ttttttccaa gaccgagata gccttccgca cggttcagta tcgtttctgc
     3541 aacacgcatg gcataaacgt cctggtgagt atatccgcct cccgactggc ccgggttgtc
     3601 aaaatggttg cagggatcag cgaatttcat gaaatacgga taaatatact gacaggaatc
     3661 tcttattgca tccctcttgt taccggcata ttcagcattg aagagagcaa acttcaattc
     3721 ctggccatga aatgctgatt cgggattatc aaaatagaat ttcctcttga tactgtgctt
     3781 tgcattccgg atatcttcat tccagccgct tctgcgccat atggtatagg ctgcataatc
     3841 agtgggtctc acccatgata ccggtctgcc gagggtgtcg gaataacctg tgtactttcc
     3901 gttaacaaac tctcccctga aagcagcttt cccgtcagga gcattgcccc atctgaaata
     3961 tgcgggaccg aatccccttt caccgctcca tgaggcacct cccgtaatca ggggctcgaa
     4021 ctgaatggcc cagatagttt ccttgttttc ggaaaggttc tggttgccat atctgaaaag
     4081 gtcataataa acgtcaccgg atccgaaaac atcatgctgt gccccgaaac gctcggtcat
     4141 aagtccgtat tcacctccgt caatcacttc tgaggcagca tcaacagcaa gctgatactt
     4201 gccctgattg aggtaaacct cagaaagcag atgccaggca gcagcctgtg tgatccggcc
     4261 gggagcctcc tcttttccgc gcccgggcaa attggccgca gcatatttca gatcatcttc
     4321 aatctgcttg taaacatcgg caaccggtgt tcttgtaaaa tctgccttgg ccgacttaat
     4381 ggcttccgtc accagcggaa cgtctccaaa atatgacaca aggatacggt atgcataggc
     4441 cctgaaaaac atggcctcgg ccttgaaggc atttctctgg gcttcacctg accatatctg
     4501 agcgtctgat gcctccactt tctcaataag cacgttggct ctctgtataa gttcatagtt
     4561 cctggtccag tactgtaata catatgctga tccgggaatc agatagttga catagttgca
     4621 caggaacctt gtgctgttgg gatcttcacc gtgaaatgca acatcggttc cgacacccct
     4681 ccacatgctg ccctggtcct gatcacccca gaaccagtaa ttgcggaatg tgtaatgcag
     4741 accggaaata ccctggcgta ttccggaaac agtggaatat gcattttcag gtgcgaggaa
     4801 atccttcgga acctccttca gtatttcctt gtcgttacag gatgacagca tcagcagtaa
     4861 aatacctgca agcaatgtac acttactgct tattataacg atatatcttt tcatcatttt
     4921 ataattttct ggtttcataa taaaaggctg aattaaaatg taacattgaa acctgcagtt
     4981 atatttctca tcaggggatt attgctggtg cccgtttcag gatcccagcc cgaccatttc
     5041 gtccacacat agggattctt gctggcaatg tagaatgtgc acgactggag cttgagcgta
     5101 ttaagaagct gtgagctgaa attgtaggac aatgaaacat cctgtaatct cacaaagctt
     5161 ctgctttcat agataccgct tgaaacagca ggtgaattat aaacacctgt tgcattgttg
     5221 accccgttgt caggtgtcca gtacggcctt acggctgagg catttattct gaggacatca
     5281 tctgaacgcc aggcaacatt gacaacagaa gcattgtcaa gcatatagta cttcttcccg
     5341 ccctgtattg aattgataaa gaaagaaagg gtgaaatttt tgtaattcag ggtattattt
     5401 atgctgaaac ggtagcttgg ggatttgtat cctacaatct tcctgtcgtt ggttggttca
     5461 atcacaccat ttttatcctg atcaacatat ttatactgcc cgggatacca gttattcaga
     5521 atatttccgc tgtagaggtc tgcttccgtc cagagtccgc ccgccatttc ataatcatag
     5581 attgcctgga tgggttctcc tacaaaccat gcgttgcc
//
LOCUS       DAOSPX010000212         5615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13138_length_5615_cov_5.523741, whole genome shotgun sequence.
ACCESSION   DAOSPX010000212 DAOSPX010000000
VERSION     DAOSPX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13138_length_5615_cov_5.523741"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="PLZ75_10015"
     CDS             complement(<1..1480)
                     /locus_tag="PLZ75_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72766.1"
                     /translation="MNIMQRKTSGLLPLERDRKRRRTLNRQRILLCMVILIMSAGNIT
                     LKGQGVGISETSIVPDPSAILELRSVVRGFLAPRMTTDQRLTLGSTSPAAGLLVYDTD
                     TKSFWYWDSGWKAFASGAWGASNQLMGMNAAGDANEYKTLTGTANRIEVTHAPGQITL
                     STPQDIHEGASPTFQGLTLSDLAPNSGVYTDHSGRLTTSVPTSGMLGYWRRSGTVLSP
                     ANSGDHITTTGNIYTTESGTISSAGLFTGSSGATISGAAINLNHDSHFPVNIGTGSST
                     GLITMGNAGNNIILPAFDSPGVVHNDASGVLFSGLISGDDIATGAIVSRHIYPGTIVN
                     EHISLDAGIDATKLIDGSISNEELRFINTLSGNAQDQLDNLSAQIDEINSLDEGMIYL
                     GDENDTAQEVRMTGDVTISNTGVTTISAESVTSGKIRDNEIVNADINSAAGIEFSKLE
                     ALPAAHIILGNSSGVPAATAISGDATIDNTGSLTLSNSGVAAG"
     gene            complement(1979..3028)
                     /gene="queA"
                     /locus_tag="PLZ75_10020"
     CDS             complement(1979..3028)
                     /gene="queA"
                     /locus_tag="PLZ75_10020"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOS72767.1"
                     /translation="MKLSQFRYNLPQELIALYPEENRDESRLLVVDRKTGKLEHRIFK
                     EIVDYFNEDDVIVFNNTKVFPARLYGNKEKTGAEIEVFLLRELNREQRLWDVLVDPAR
                     KIRIGNKLYFGEDDLLVAEVIDNTTSRGRTLRFLFDGSYEAFKQTLYSLGETPLPKFI
                     NRPVEPGDSERYQTIFAKHEGAVAAPTAGLHFSRELLKKLEIIGVEFAEITLHVGLGN
                     FRSVDVEDLTKHKVDSERIIITEETAEIVNKARDNRKKICAVGTTVVRTLESSVSTDG
                     YLKPFDGWTNKFIFPPYDFKVPDMMISNFHLPYSTLLMMVAAFAEYDLLFEAYRTAVA
                     EKYRFGTYGDAMLIL"
     gene            complement(3074..3787)
                     /gene="truB"
                     /locus_tag="PLZ75_10025"
     CDS             complement(3074..3787)
                     /gene="truB"
                     /locus_tag="PLZ75_10025"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOS72768.1"
                     /translation="MDKPEAENIFQEGRVLLFDKPLYWTSFDLVNKVRIMIRKAFGLK
                     KLKVGHAGTLDPLATGLMIICTGKATKGIEEYRELDKEYYANLYFGKTTASFDLETET
                     EGDYPVEHITREIVENVLKGFKGEQKQVPPLHSAKLINGKRAYEYARSGKNLELSPVN
                     VIIRETELVSFGIPEMQIRILCSKGTYIRAFARDLGHALGSGCYLSALRRSSIGDFKL
                     QDALDIKKFEKILEELKQT"
     gene            complement(3789..4568)
                     /locus_tag="PLZ75_10030"
     CDS             complement(3789..4568)
                     /locus_tag="PLZ75_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOS72769.1"
                     /translation="MSLLEAILLGIVQGLTEFLPVSSSGHIEIGKVLFGVDAESSFYF
                     TVAVHGATVLSTIAVFRKDIGALLSGFLKFRMNDETSYILKILVSMIPVGFVGLLLKD
                     QVEALFDGNLVFVGSMLIITAALLATAHFIKKRERQIGYLDALIIGVAQALAVIPGIS
                     RSGATIATGLVIGNRKDEIARFSFLMVLIPVLGANFLELLRTESTMAGPGTAAILAGS
                     LAAFISGYFACRWMIGLVRKSKLIGFSVYCAAAGLLAILLG"
     gene            complement(4759..5010)
                     /locus_tag="PLZ75_10035"
     CDS             complement(4759..5010)
                     /locus_tag="PLZ75_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOS72770.1"
                     /translation="MATKKEEKEKPDFALGPGNYKLLGIGFLIIVVGFLLMLGGKTKD
                     PAEFSNDIFSFRRITLAPIVVLAGFIFEIWAIMRKPKEK"
     gene            complement(5017..>5615)
                     /locus_tag="PLZ75_10040"
     CDS             complement(5017..>5615)
                     /locus_tag="PLZ75_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS72771.1"
                     /translation="MKEELGEDFISFLGENPLPPSIDVYLVSEYTRPDSVTKIEKYIL
                     EYPFVKEVYYQESLLYLINENVNKISLFLLIISFFLFLIALTIINNTIRLSIYSRRFL
                     IRTMQLVGATRSFIRKPFIIQSAFHGFLAALTAMLLLMGLLYMIEKEFFLMFTYENTF
                     LLILLGLAIIVTGIIINMVSTYFSVNRYLSISEDKLYY"
BASE COUNT         1460 a         1333 c         1199 g         1623 t
ORIGIN      
        1 aacctgccgc gacgccgcta ttcgataaag taagggaacc ggtattgtca attgtagcat
       61 ccccgctgat ggctgttgca gcaggaactc cggatgaatt gcctaaaata atatgggctg
      121 cgggaagagc ttcaagtttt gaaaattcta ttcctgcagc actgtttata tctgcattga
      181 ctatctcatt gtcacggatc tttccggaag taactgattc ggcacttatc gtggttaccc
      241 cggtattgct gattgtcaca tcgccggtca tacgaacttc ctgcgccgta tcgttctcat
      301 cgcccagata aatcatgccc tcgtcaagtg aatttatttc atctatctgt gcggacaaat
      361 tatcgagctg gtcctgtgca tttcctgaaa gggtgttgat aaaacgcagc tcctcattgc
      421 tgattgatcc gtcaataagc ttggtggcgt caatacctgc atcgagactg atatgttcgt
      481 ttactatcgt gcccggataa atatgtctgc tgactatggc tccggttgct atatcatctc
      541 cgcttatcag tcctgaaaaa agcacgccgg atgcatcgtt gtgcaccact ccgggtgaat
      601 caaatgccgg caggatgata ttgtttccgg cattgcccat tgtgataagg cctgttgaac
      661 tgcctgttcc tatgttcacc gggaaatgtg aatcatggtt caggttaatt gcagcacccg
      721 aaatagttgc acctgaactg ccggtaaaca agccggctga gcttattgtg cccgattccg
      781 tggtatagat atttcctgtt gtggtgatat ggtcaccgga atttgccggt gaaaggactg
      841 ttccgctacg tctccagtaa ccaagcatcc cgcttgtggg aacactcgtt gtcagtctgc
      901 ctgaatgatc agtatataca cctgaattgg gtgcaagatc acttagtgtc aatccctgga
      961 atgttggtga cgctccctca tgtatatcct gcggagttga taaagtaatc tgcccgggag
     1021 catgtgtaac ctctatccgg ttagctgtcc ctgtaagggt tttatattca ttggcatcgc
     1081 ctgcggcatt cattcccatc agctggtttg atgcacccca ggcaccggag gcaaatgcct
     1141 tccagcctga atcccagtac cagaaagatt ttgtgtctgt atcataaacc agcagacctg
     1201 cagccggaga tgtgcttccc agagtcagcc gctgatccgt tgtcatgcga ggggcaagaa
     1261 aacccctcac aaccgacctc agttccagta ttgccgaagg atccggtaca atggaggttt
     1321 cactgattcc cacaccctga ccctttagcg taatatttcc ggctgacatt atcaatatta
     1381 ccatgcataa gagaatcctt tgccggttca gggtccgcct ccttttcctg tcccgctcaa
     1441 ggggcagaag acctgaagtt tttctttgca taatattcat gatatacctc ctgaattttt
     1501 gggtatttgg attttaatat ctgcgaaaca tggagaatac ccggacaaag ctgatccggg
     1561 gaaaggacgt aacagctcaa ctgttttgat ttctatacta tagagacctg cagacgccat
     1621 caggttaaat aacaacaata tttccggttc attttattaa aatccccgga cgatatctgt
     1681 ttatataatt tatgctccga ctttcacaat gatgttaaac gtaccatata ttaatatgtt
     1741 actaaaaaat ccagggtttt taatattgta ttgcattatt tgatagaggc ttgccggacc
     1801 ggccagattt tccggacagg tcagcaggat cctttccccg gggtgaaaag tcagggtccg
     1861 ggaaaaaact caacaaaaaa gccggataga taaatttatc cggctgaata tcattataat
     1921 aaatgaatct gttcagtatg aaccggcttc ttcgccgggt cattttcagc atttgccttt
     1981 acaggatcag cattgcatca ccataggtgc cgaacctgta tttttccgcc actgcggtac
     2041 ggtatgcctc aaaaagaagg tcatattccg caaatgcggc caccatcatc agtaatgtcg
     2101 agtatggaag gtggaaatta ctgatcatca tgtcgggcac tttaaagtcg tagggaggga
     2161 aaatgaattt attggtccac ccgtcaaagg gcttgagata tccgtcagtt gacactgaac
     2221 tttcaagggt cctgacaacc gttgttccca ctgcacaaat cttcttcctg ttgtccctgg
     2281 ctttgttgac tatctcggca gtttcctcgg taataatgat cctttcagag tcaaccttgt
     2341 gctttgtgag gtcttcaaca tccacgctcc tgaagtttcc aagaccgaca tgcagtgtta
     2401 tctcggcaaa ttcaacacct attatctcaa gcttcttcag cagttccctg ctgaaatgga
     2461 gtcctgcagt cggtgctgca accgcgcctt catgcttggc aaagatggtc tggtatcttt
     2521 cactgtcgcc cggctcaact ggcctgttaa taaatttggg taggggggtt tcgcccagac
     2581 tgtaaagtgt ttgcttaaat gcctcatagc ttccgtcaaa aagaaacctg agtgtcctgc
     2641 ccctggatgt cgtattgtca ataacttcag caacaaggag atcatcctca ccaaaataca
     2701 gtttgttccc tatccttatt ttgcgtgcag gatccacaag cacatcccag agcctttgtt
     2761 cgcggttcag ttccctcaga agaaacactt ctatctcagc gccggttttt tctttattcc
     2821 cgtatagcct ggcaggaaaa accttggtat tgttaaaaac aattacatca tcttcattga
     2881 aataatcaac tatttccttg aatatcctgt gctcgagctt tcctgttttc ctgtctacaa
     2941 caagcaacct cgattcgtcc ctgttttctt cggggtatag ggcaataagt tcctgtggta
     3001 agttgtacct gaattgtgat aatttcattt ccagcctggt atttatttta agaatccttt
     3061 atacgtataa atttcatgtt tgtttcaatt cttctaaaat tttttcaaat ttttttatgt
     3121 caagtgcatc ctgaagttta aaatcaccta ttgaacttct cctcagtgct gacaaataac
     3181 agccgctgcc aagtgcatgg cccagatccc ttgcaaatgc tcttatatat gttcccttgc
     3241 tgcagaggat tctgatctgc atttccggga ttccgaatga aacaagttcc gtttccctta
     3301 ttataacatt tacgggcgat aattccaaat ttttcccgct tcttgcgtac tcataagccc
     3361 gcttcccgtt tatcagtttt gccgaatgga ggggtggaac ctgcttctgc tctcctttaa
     3421 agcctttcag aacattttca acaatttccc gggtaatatg ttcaacagga taatctcctt
     3481 cagtttctgt ttccaggtca aatgaagcag tggtttttcc aaaatacagg tttgcataat
     3541 attccttgtc cagttcacgg tattcctcta ttccctttgt tgccttcccg gtacagatga
     3601 tcatcagtcc ggtggcaaga ggatcaaggg ttccggcatg ccccaccttt aattttttca
     3661 gtccgaaagc cttccggatc attatcctga ctttgtttac caggtcaaat gatgtccagt
     3721 aaaggggttt gtcaaacaga agaaccctgc cttcctgaaa aatattttcc gcctccggtt
     3781 tgtccatatc agccaagcag tatagccagt aatcctgcgg cagcacaata gacagagaat
     3841 cctatcagtt tgcttttccg caccagtccg atcatccagc ggcaggcaaa atagcctgaa
     3901 atgaatgcag ccagtgaacc ggccagtatc gcggctgttc cgggtccggc catggtgctt
     3961 tccgtcctga gcagctcgag aaagttggcg cccagaaccg gtatcagaac catcagaaaa
     4021 gaaaagcgtg ctatttcatc ctttctgttt cctataacca gtccggtggc aatggttgcg
     4081 cccgaccttg aaatacccgg aatgacagcc agtgcctgtg ccactccgat gatcagtgca
     4141 tccagatagc ctatctgcct ttcccttttc tttatgaaat gtgccgtcgc cagcaaagct
     4201 gccgtaataa taagcattga accgacaaat acaaggtttc cgtcaaacag ggcttcaacc
     4261 tgatccttca ggagcaggcc tacaaatccg accggaatca ttgaaacaag aatcttcagt
     4321 atataggatg tttcatcgtt catcctgaat ttcagaaatc cactgagaag tgctccgatg
     4381 tccttccgga ataccgcaat tgtgctcagt actgttgccc cgtgaaccgc cacggtaaaa
     4441 tagaagcttg actcagcgtc aacaccgaac agtaccttgc ctatttcaat atggccggaa
     4501 ctgcttacgg gaaggaactc tgtgagaccc tgtacaattc caagtaatat tgcttccaat
     4561 aaactcatga tcaatataat taaaaaaatc taaagcgggc cggaacagaa aaggcatgaa
     4621 gcagatctgc tggtaccggt cccggttgcc gggaatgcag ccggatattc acggcacccg
     4681 tgttccgcgg gaaataaagg atattacggt cgtcaaggat gccatgaaga ttccggacac
     4741 tcaggccggg cagtttagtc acttttcctt tggttttctc atgatggccc agatttcaaa
     4801 aataaaaccg gccagtacta cgatgggtgc cagggtgata cgcctgaagc tgaatatatc
     4861 gttactgaat tcagcaggat ctttcgtttt ccctcccagc atcagaagaa acccgacaac
     4921 gattatcagg aatcctattc ccagaagttt gtaatttccg gggccaaggg caaaatccgg
     4981 tttttctttt tcttcttttt tagttgccat atgatgtcag taatatagtt tgtcttcaga
     5041 aatgcttaaa taacggttta cggaaaaata ggttgagacc atattgatga ttattcctgt
     5101 cacaatgatt gcaagtccga gcaatatcag cagaaaggtg ttttcatagg tgaacatgag
     5161 gaaaaactcc ttttcaatca tataaagcag tcccatgagc agcagcatcg cggtaagggc
     5221 cgcaaggaac ccgtggaaag cactctgtat gataaagggt ttcctgataa atgagcgggt
     5281 tgcacccacg agctgcatgg tcctgataag aaatcttctg gaatatatcg aaagccttat
     5341 tgtattatta atgatcgtaa gagcgataag aaacaggaag aaactgatga tcaggaggaa
     5401 caggcttatt ttgtttacat tctcgtttat caggtaaaga agcgactcct ggtaatacac
     5461 ttccttgaca aagggatatt ccaggatata tttttcaatc ttcgtaacgc tgtccggcct
     5521 ggtatattcg gaaacaaggt acacgtctat tgaaggcggc agagggtttt ccccgagaaa
     5581 actgataaaa tcctctccca gctcctcctt cattt
//
LOCUS       DAOSPX010000213         5600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13200_length_5600_cov_3.139225, whole genome shotgun sequence.
ACCESSION   DAOSPX010000213 DAOSPX010000000
VERSION     DAOSPX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13200_length_5600_cov_3.139225"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PLZ75_10045"
     CDS             complement(<1..1087)
                     /locus_tag="PLZ75_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOS72772.1"
                     /translation="MKLKGTKFVDNISGLQIFQLLRFTIFLIISIVFTKSHLSSADIG
                     KWEMFLFIAGMLTFFWVTGIIQSLLPLYHRNRTYRKMGDPDRGKSPEIFNAFVLLTLF
                     SLFVFILGHSLKGSISVFHFSGNVPFINLLLLYILLSSPVCLIEYIYLLNNRSHRILQ
                     YGFYTFSVQLVMIIVPVLAGKDIIWSVYGLLAITLVRWVWLIILLRRYSKPEISVKFM
                     KEHLYLGWPLIITSLISGSAQYVDGVIISSVYRDPALFAWFRYGAKEFPLVLLLANGL
                     SNAMLPEFSVRAKMRESLAKLKLRSEKLMHILFPVTMVIMFFTRWFYPRLFAPEFHRS
                     AGVFLVYALLFIPRLVFPQTIVIGRRKT"
     gene            1251..1326
                     /locus_tag="PLZ75_10050"
     tRNA            1251..1326
                     /locus_tag="PLZ75_10050"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1286..1288,aa:His,seq:gtg)
     gene            complement(1429..3636)
                     /locus_tag="PLZ75_10055"
     CDS             complement(1429..3636)
                     /locus_tag="PLZ75_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72773.1"
                     /translation="MKHIRIVLFLFLFSGIASAQLLSGDYRHLPVYNPSPAVSGDWLL
                     DDSDRKADIYVSADGKNIIMSNGLAERIFTLSPDVACISYRNLTTGRQMLRAVSPEAI
                     VTIDLIPYPIGGLEGQPERAYLKPEWIPAMTSPDNEFRLADIRISGIRPYVSWKPRRW
                     SGRQSLPAGKTLTFIFKPAGTSRIKGVSVMVNYEIYDGIPLMNKWVEVINDGNSSLVV
                     NRVVSEKLALTEEESAVVGGPEKMLKPHYLYVESNYAFNNAMNYHLSDQTTHWKRDYG
                     YTSQVNYNLDTPCILEIYPHTDPNIRLEKGEQFRSPHSFQLLFDGYDRERNGLAVRRM
                     YRTVAPWVTENPIFMHLVSRSDDQVYTAIDQCAATGYEALILSFGSHINIEDNSPGNV
                     AKMKRMADYAHERNILIGCYSLFSSRTIGPETDVIDPETGKPGGAFFGNAPCLGSEWG
                     LKYIENLKYMFTETGFDIFENDGPYPGDVCASTSHPGHEGLHDSQWRQMEIQKSLYRW
                     CNENGIYVNAPDWYFLDGTNKIALGYRETNFSLSRDRQRILNRQNIYDGTWLKTPSMG
                     WGFVPLTAYHGGGPDAVLEPLSEHLDDYEQLMMQYYGAGVQACYRGPRLYDTGETRAV
                     VQRVVNWYRKYRDILNSDIIHLRRPDGSDWDGIMHVNPRLREKALAMLYNPLDNDITR
                     VIRLPVYYTGCHDRLSVRTGEGKSGRYRVDRDYNVTITVTIPAGSYIWLVMEE"
     gene            complement(3710..>5600)
                     /gene="ccsB"
                     /locus_tag="PLZ75_10060"
     CDS             complement(3710..>5600)
                     /gene="ccsB"
                     /locus_tag="PLZ75_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOS72774.1"
                     /translation="LKYKGYRFYQSSYDPDEMGTILSVNHDRAGMIVTYTGYSFLFFF
                     ILLSLLIRKSLFRTVRATMWTSALRKSLILLLFFTVLSGLGSISAQQFVPGKKASDEL
                     GKILIQDQKGRTKPLYTMSNDIVRKVTGGNKFGNYSSMQFFLGFLLDFDHWKEMPVIK
                     VNNEGVRKKAGISGRYASFSDLVDFSGEGHYKLSEDVRAAYSKSPGSRNKTDKEVMKV
                     DERVNIIFMISRGDFLRIFPLNDTTHNWGPPHEALVNAPDHADSLYLQNIIPALVNSV
                     QENNKMKAAQVVSSVRIYQERFAEYKLPSVRRVVSEVLYYRLVVFERLFPLYLFAGLA
                     MIVILIITVITNRPLNSPVLRILGWLLVAGFTLHTSGLALRWYISGHVPLSNGYESMI
                     FISWITLLAGLIFRRRSEFALSSTAVLAGLTLLVAHMSFMDPEITNLVPVLKSYWLTL
                     HVSVITGSYGFFGLGALLGLLTMVLMMLTREGNRERISSTIDELTVINYKTLTLGLYF
                     LTIGTFLGAVWANESWGRYWGWDPKETWSLITMVIYSLVIHSRMIPGMRDVYSFNLLS
                     LFAFSSVLMTYFGVNYYLSGLHSYAGGDPVPVPAFVYVTVIILVSVSVAAYIRYRSFR
                     KTVQE"
BASE COUNT         1509 a         1385 c         1293 g         1413 t
ORIGIN      
        1 gtgtttttct cctgccaatc acgatggttt gcggaaaaac gagtcgcgga ataaaaagga
       61 gggcatatac aagaaaaacc cctgcgctgc ggtggaattc tggtgcaaac aaacgcggat
      121 agaaccagcg ggtaaagaac atgatcacca ttgtcaccgg aaaaagaata tgcatcagtt
      181 tttctgacct gagtttaagt tttgccagtg attccctcat tttagccctt accgagaatt
      241 ccggaagcat tgcattgctc agtccgtttg ccagcagaag caccagcggg aattcctttg
      301 caccatagcg gaaccaggcg aacaatgcag gatcgcgata caccgaggaa ataatcactc
      361 cgtcaacata ctgggccgat ccgctgatga gtgaagtgat tatcagcggc catcccagat
      421 acagatgctc cttcatgaat ttcactgata tctcgggttt tgaatagcgt ctgagaagta
      481 ttataagcca tacccagcgt accagtgtta ttgcaagcag gccgtaaacc gaccatataa
      541 tatccttccc ggccagaacc gggactatga tcatcaccag ctgcaccgag aatgtataga
      601 aaccgtactg caggatacgg tgcgacctgt tgttcagaag atagatatat tctatcaggc
      661 agaccgggct gcttaaaagg atgtacagca gcaaaaggtt tataaaggga acattacccg
      721 aaaaatgaaa caccgaaata cttcctttca gggaatggcc caggataaat acaaagagac
      781 tgaatagtgt aagaagaaca aaggcattga atatttccgg cgacttcccc ctgtccgggt
      841 cacccatctt cctataggtc cggttccggt gataaagtgg cagaagcgac tgtattatcc
      901 ccgtaaccca gaaaaaggtg agcattccgg caatgaacag gaacatttcc cactttccta
      961 tgtcggcact gctgagatgg ctcttggtaa atacaatact gattataaga aagatggtaa
     1021 accttaacag ctggaagatc tgtaaaccgg agatattatc gacaaatttt gtccctttca
     1081 gcttcatctt gttggaatgg agaatcttcc ttaattaaat atggcgggca tgaaccgatt
     1141 ccgcggttcc cgtaaaaacg gcacaaatat agcaattgac gggaagcata agaaattaat
     1201 ttaaaatttt gtcaataaaa aaattaaggc tacttttgca ttcctgaaac ggtggatgta
     1261 gctcagttgg ttagagtacc ggattgtggt tccgagggtc gtgggttcga atcccatctt
     1321 ccacccttca aaaaacaaac gagggcagtt gtctgaatat atgacaattg ccctttattg
     1381 tatggtcacc cgcacgggaa gcaacatgcg attcagctgc cttcaatgct attcctccat
     1441 aaccagccag atatagctgc cggccggtat ggtgacagtg attgttacat tgtaatccct
     1501 gtccaccctg tacctgccgg attttccttc ccctgtacgc actgaaagcc tgtcgtggca
     1561 acctgtataa taaaccggta gtctgataac acgggtaatg tcattatcca ggggattgta
     1621 aagcatggcc agggcttttt ccctgagacg gggattgaca tgcatgatgc cgtcccagtc
     1681 gcttccgtcc ggacgacgca ggtggataat atccgagttg aggatgtcgc ggtatttcct
     1741 gtaccagttc accacccgct gcacaaccgc ccgggtttcg ccggtgtcat acagccgggg
     1801 accgcggtag caggcctgga ctcctgcacc gtaatactgc atcatcaatt gttcataatc
     1861 atccaggtgt tcgctgaggg gttccagcac tgcatccgga cccccgccgt gatatgctgt
     1921 cagcggaacg aagccccagc ccatcgaggg cgtcttaagc caggttccgt catatatgtt
     1981 ctgacggttg agtattctct gcctgtctcg tgacagggag aaattggttt cacggtaacc
     2041 cagtgcaatc ttgttggtgc catccaggaa ataccagtcg ggtgcattca catatatccc
     2101 gttttcattg caccagcggt ataaggattt ctgaatctcc atctgcctcc actgcgaatc
     2161 atgcagtccc tcatgaccgg gatgtgatgt ggaagcacat acgtcgcccg gatagggacc
     2221 gtcattctcg aaaatatcga agcccgtctc cgtgaacatg tatttcaggt tttcaatgta
     2281 tttcagtccc cattcacttc ccaggcacgg agcattcccg aagaaagcac cgcccggctt
     2341 tcccgtctca ggatcaatca catccgtctc agggccaatg gtccggctcg aaaacagtga
     2401 atagcagcca atgagaatat tcctttcatg ggcataatcc gccatccttt tcatctttgc
     2461 aacattcccc ggcgaattgt cttcaatatt tatatgactg ccgaaactga ggatcagagc
     2521 ttcataaccc gtggcagcac actggtcgat ggcagtatat acctggtcgt cgctgcggct
     2581 cacaaggtgc ataaagatgg gattttccgt tacccagggt gcgactgtcc tgtacatacg
     2641 gcggacagcc agtccgttac gctcacggtc atagccgtca aacagcagct ggaagctgtg
     2701 aggagaacgg aattgctctc ctttctcaag acgtatattg ggatcggtat gcgggtatat
     2761 ctccagtatg cacggagtgt ccaggttata attcacctgg gatgtatagc cataatccct
     2821 tttccagtgc gtggtctgat cgcttaaatg ataattcatt gcattgttga aggcataatt
     2881 gctttcaacg tacaggtaat ggggtttcag cattttctcc ggtcctccca ccactgccga
     2941 ttcctcttcc gtcagtgcca gtttttcgct caccaccctg ttgacaacca gtgatgagtt
     3001 cccgtcattg attacttcca cccatttatt catgagagga atgccatcat agatttcata
     3061 gttcaccata acgctcacac cctttatacg ggaagtgcct gccggcttaa agatgaaagt
     3121 gagcgttttt cccgcgggaa gactttgccg gccgctccac cgccgcggct tccagcttac
     3181 atagggccgg ataccggata tccttatatc tgcgagcctg aattcattat ccggggatgt
     3241 cattgccggg atccattccg gtttcagata agccctttca ggctgtcctt ccagaccgcc
     3301 aatgggataa gggatcagat caatggtgac aatggcttcc ggactgacag cacgcagcat
     3361 ctgcctgcct gtcgtcaggt ttctgtaact gatacaggca acatcgggtg aaagggtgaa
     3421 tattcgctcc gccaggccgt tggacataat gatatttttc ccgtcagcgg aaacatagat
     3481 atccgcctta cggtcactgt catccagcag ccagtcgccg gatacggcgg gcgacggatt
     3541 gtaaacaggc agatgccggt aatcaccgga aagcaattgc gcactggcta ttccgctgaa
     3601 aagaaacagg aaaagaacaa ttctgatatg cttcatgata aggtattagt gaaattcatt
     3661 caaaatcagg gaaaaaccct gattcagcct gcaggatgac aaaatacgat cattcctgta
     3721 ctgtttttct gaaacttctg tatcttatat atgccgcaac cgacactgac acaagtatga
     3781 ttaccgtgac atatacgaaa gccggtaccg gtacaggatc acctcccgcg tatgaatgaa
     3841 ggccagagag atagtagttg accccgaaat aagtcatcag cacggatgag aatgcaaaaa
     3901 gcgagaggag attgaatgaa taaacatccc tcattcccgg aatcatgcgt gaatgaatga
     3961 caagcgaata tattaccatg gtaatgagtg accatgtttc cttgggatcc cagccccagt
     4021 accttcccca ggattcattc gcccagactg ctcccaggaa tgttccgatg gtaaggaaat
     4081 acagtcccag ggtcagggtt ttataattga tcacggtcag ttcgtcaata gtggaagata
     4141 ttctttccct gtttccctca cgtgtcagca tcataagcac cattgtcagg agtccgagca
     4201 gtgccccgag cccgaaaaac ccgtagctgc cggtaataac agacacatgc agggtgagcc
     4261 agtatgactt aagcacgggt accagattgg ttatctcggg gtccataaag ctcatatgcg
     4321 ccactaggag tgtcagtccg gccagaaccg cagtggaaga gagtgcaaat tccgatctgc
     4381 ggcggaatat aagacctgca agaagggtta tccatgaaat gaaaatcatg gattcatatc
     4441 cgttgctcag aggaacatgt ccggaaatat accaccgcag ggccaggcct gatgtatgga
     4501 gtgtgaatcc tgccacaagg agccagccca gtatcctgag aacgggtgag ttaagcggtc
     4561 tgttcgtgat aaccgttatt atcagaatga caatcattgc aagtcctgcg aaaagataaa
     4621 gcggaaagag cctctcaaaa acaacaagcc tgtaatagag tacctcagaa acgacacgcc
     4681 gtactgaagg cagtttatat tctgcaaatc tttcctggta tatccttacc gaggaaacaa
     4741 cctgggctgc tttcatttta ttattctcct gaaccgaatt taccagtgcg ggtattatat
     4801 tctgaagata cagtgaatct gcatgatcag gtgcattgac cagggcctca tgcggagggc
     4861 cccagttatg ggtagtgtca ttcaggggaa atattctcag gaaatcaccc ctggatatca
     4921 tgaagatgat attaaccctt tcatccacct tcataacctc cttgtccgtt ttgttcctgc
     4981 tgcccggtga ttttgaatat gccgccctta catcttccga aagtttataa tgaccctccc
     5041 cgctgaagtc aaccaggtcg gaaaatgatg cataccggcc gcttattcct gcctttttcc
     5101 ttaccccctc attattcacc tttattacgg gcatctcctt ccagtgatca aaatccagca
     5161 ggaatcccag gaaaaactgc atggatgaat aattcccgaa cttattcccg cctgttactt
     5221 ttcttacaat atcattgctc atggtgtaca ggggcttggt tcttcccttc tgatcctgaa
     5281 tcagtatctt tcccagctca tccgaggcct ttttcccggg aacgaactgc tgtgccgaaa
     5341 tgcttccgag tcctgacaaa acagtaaaga acagcagaag gatcaggctt ttcctgagtg
     5401 cagaagtcca catcgtggcc ctgaccgtac ggaacaggga tttcctgatc agcagtgaaa
     5461 gcaggatgaa aaagaaaaga aaggaataac ctgtataggt aacaatcatt cccgccctgt
     5521 cgtggttaac tgacaatatc gtacccattt catcaggatc gtatgaagac tggtaaaacc
     5581 tgtaaccctt gtacttaagc
//
LOCUS       DAOSPX010000214         5527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13439_length_5527_cov_4.255117, whole genome shotgun sequence.
ACCESSION   DAOSPX010000214 DAOSPX010000000
VERSION     DAOSPX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13439_length_5527_cov_4.255117"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLZ75_10065"
     CDS             complement(<1..408)
                     /locus_tag="PLZ75_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOS72775.1"
                     /translation="MGKYKLLILTGLLSAFTAISAGQAGSMAFDLVFIGNSITQGDSE
                     NDSPPASAAEHLRSRKGVESVRFLNRGRSGYTTVNFLPVPDGELMRIISATEEFHKDP
                     GRQLVFSIMLGTNDSAEEGPMGAPVSPGQYYFNM"
     gene            604..858
                     /locus_tag="PLZ75_10070"
     CDS             604..858
                     /locus_tag="PLZ75_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72776.1"
                     /translation="MTTLIPISVSSWSGYKADEYPKSFSLQGKKYQILEITDRWYQGD
                     MNPEYPAASYFRVTTVEGGRFILKHETGTDTWYLIREKPA"
     gene            1116..2306
                     /locus_tag="PLZ75_10075"
     CDS             1116..2306
                     /locus_tag="PLZ75_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS72777.1"
                     /translation="MQNRRRFIRNSLLTVAAAGLPVRLGKAARNLSEAEGRPLIRTLG
                     RTGISLPVVSMGTSSTTSPALIRAALDNGIVLFATSAAYQEGNNEEMLGEVFKNFPRD
                     SFFILTNSFDIQWIDTPTGILKPGFSRDELLKRAEGSLKRLGVEYVDFFTQPFAARRE
                     SVIHDQALRAMEALKQEGITRFTGIATHRYEPEAIRAAAESGIHDFVMTSYNFLKENR
                     EELGEAIQYAAEAGLGIIAMKTMAGAYYDKERTKPVNTRAALKWVVRNSNIHTTVPDC
                     TSFEQLSQNIRVMAEPELNDEEWKDLFPPSGTHFSEIFCQQCGICLQNCPCNVDIPAL
                     MRSYMYLYGYGNRAQAKSTLAEAGMAYNPCSGCSACLANCRMGHDIRKKICDISGILG
                     LPEG"
     gene            complement(2516..3436)
                     /locus_tag="PLZ75_10080"
     CDS             complement(2516..3436)
                     /locus_tag="PLZ75_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72778.1"
                     /translation="MSKISDDILKQIQSEIDRIGEAPSSLDELNKIAANVVDRFNNAP
                     KSDFEGLSPYQMHILLHRPFSPECIVKFGASTGINVADKSPVVKISLLIINAIIAQNG
                     LALTKGGKLPRKIVDAIYNLNFHSTNSIPGYYKRTLNELDYAPAAITNALLKLSGLVI
                     IRKNKLHLTKAGKSAEVDHMMLFRSLFTAFSTRFNKGYLDGYGSDTIGNLGTLYTIYL
                     LNSFGKKRLEASFYARLYFKAFPELINEVTYYAFSTPEKTAYDCFLYRIFEKGLLLFG
                     LVEIEFEGKDYFDRKMYIKTSDLFHQIFKI"
     gene            4181..4783
                     /locus_tag="PLZ75_10085"
     CDS             4181..4783
                     /locus_tag="PLZ75_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72779.1"
                     /translation="MGYQTPAGDSCIPLQMAFYRQFDPELNESTFRWRLYYLKSKGII
                     TPISQDLLTLNYKPVFKPDVSDVEEIASKAEKQFSTMKYCIWSTKVISEFMLHIPGKF
                     MTVLEVEQDALEPLYEYLKIQNYNNVYIGPEEKEIERYIFESEQPIILQSLISKTPIQ
                     KVGKIYTVTLEKLIIDLFCDKHLFIAFRYILSKRMKVFVL"
     gene            5300..>5527
                     /locus_tag="PLZ75_10090"
     CDS             5300..>5527
                     /locus_tag="PLZ75_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOS72780.1"
                     /translation="MEQIPMTLSDFLKNKRKQLNLTQQQLAEKAGVGLRFIRDLEQGK
                     TTLRMDKVNQVLKLFGQELGPQPINREKLLRK"
BASE COUNT         1689 a         1138 c         1113 g         1587 t
ORIGIN      
        1 catgttgaaa taatattgtc ccgggcttac aggtgctccc atcggtccct cttccgcact
       61 gtcgtttgtc ccgagcatta ttgaaaacac cagctgacgg ccgggatcct tatgaaattc
      121 ttccgtggcg gaaatgatcc tcataagctc accatcgggt acgggcagaa aattgactgt
      181 cgtatatccg ctcctgccac ggttaagaaa gcggaccgat tccacacctt tcctgctccg
      241 gagatgctcc gccgcactcg cgggaggaga atcattttca ctgtcaccct gggtaatgct
      301 gttgccgata aaaaccagat caaatgccat gctccctgcc tgcccggctg agatggctgt
      361 aaaagcagac agcagtccgg tcaggattaa taatttatac tttcccatgt cagaatagtt
      421 ataagaacac caggagaaaa ataaaaataa aaacccggaa tctgaaatga atccccggtt
      481 tttatccctg aggccccgga ttaaacagga aagattttca gttcatactg ttttccgctg
      541 actgaaaaag agtaaatacc gtatcccgtt catatctttg cagaacgtgg ttctgttccg
      601 cagatgacaa ctttgattcc catatcagta agcagctggt ccggctataa agctgacgaa
      661 tacccgaaaa gcttttccct gcagggaaaa aagtatcaga ttctggaaat caccgaccgc
      721 tggtatcagg gcgacatgaa cccggaatat ccggctgcca gttatttcag ggtaacaacc
      781 gtcgaaggag gccggttcat cctgaaacat gaaaccggaa ccgacacctg gtacctgatc
      841 agggaaaaac ctgcctgacc agttgttccg caactttacc cgtggaaaaa accggtttac
      901 ctggcgctcc gcattgccct gcaggaacag gattatgcgt tataatcctc tcatgaagga
      961 aagagccccg tttataagca ttgtaaatta ttttttccgg attcatatta tatgttataa
     1021 agctttctga atgaaaaatc tgtttatgac attgaatttt ccgctgagat ttcttaactt
     1081 tttcattgta aaagaccgga taaattgaaa aagtcatgca gaatcgacgc agattcatca
     1141 gaaacagcct gttgacagta gcagctgccg gattgccggt cagattagga aaagccgccc
     1201 ggaacctgtc ggaagctgaa ggcagaccat taatccgtac gcttggcagg accggtatca
     1261 gtctgccggt tgtaagcatg ggaacaagca gtacaaccag tccggctctt ataagagctg
     1321 ctcttgacaa tggcatagta ctgtttgcca cctctgcagc atatcaggag ggtaacaacg
     1381 aggaaatgct gggagaggtt tttaaaaatt tcccgagaga ttccttcttt atccttacaa
     1441 attcctttga tatacaatgg atagacactc ccacaggtat tcttaaaccc ggcttctccc
     1501 gggacgaatt gctgaaacgt gcggagggca gcctgaaaag acttggagtt gaatacgttg
     1561 atttcttcac ccagcccttt gccgccagac gggaatctgt tattcacgat caggcgctga
     1621 gagcaatgga agcattgaag caggaaggaa taaccaggtt cacaggtatt gccactcacc
     1681 gctatgaacc tgaagcaata agggctgcag ccgaatccgg tattcatgat tttgttatga
     1741 caagctataa cttcctgaaa gaaaacaggg aagaactcgg agaagcaata caatatgctg
     1801 ctgaagccgg actgggtatt attgcaatga aaacaatggc cggagcatat tacgacaagg
     1861 aaagaacaaa gcctgtcaat acacgtgcag ccctcaaatg ggtagtcaga aacagcaata
     1921 tacataccac agtgcccgac tgcacaagct ttgaacagct ttcgcagaat atcagggtaa
     1981 tggcggaacc ggaactcaat gatgaggaat ggaaggatct gttccctcca tcgggaacac
     2041 atttctcgga aatattctgc cagcagtgcg gcatatgcct gcagaactgc ccctgtaacg
     2101 tggacattcc ggctcttatg cgcagttata tgtatttata cggctacggc aacagggcac
     2161 aggccaaaag cactcttgcc gaagccggaa tggcatacaa tccatgttca ggctgttctg
     2221 cctgccttgc aaattgcaga atgggccatg atatcaggaa aaaaatatgt gatatcagcg
     2281 gaatactcgg attaccggaa ggatagaaaa agaatcccaa ttattcaagt tacaatggca
     2341 gaatgaataa gtccgccgtt gatttctccc taataaaaag agcgttatat tttattgatt
     2401 ttcaacgatg tcctgtctga atcaatcggg ataagaaacc gacaaatatc gtattgacca
     2461 gacatagaga tatatacggc tgatatacga ctgctaatca gccttccaaa aactattata
     2521 ttttgaaaat ctgatggaat aaatcgctgg ttttaatata catttttctg tcaaaataat
     2581 ccttcccttc aaattcgatt tcaaccagtc caaacagtaa caatcccttc tcaaatatcc
     2641 tgtacaaaaa gcaatcatat gctgtttttt ccggagtcga aaaagcgtaa tatgttactt
     2701 cattgattaa ttcagggaaa gccttaaaat aaagcctggc ataaaaagat gcttccaaac
     2761 gctttttacc gaacgagttt aacagataaa tcgtatataa tgttcccaaa tttccaattg
     2821 tgtcacttcc atacccgtca agatatcctt tgttaaatct tgtggagaat gctgtaaata
     2881 agctcctgaa taacatcata tgatcaactt cagcactttt gcctgctttt gtcaaatgaa
     2941 gcttgttttt ccggattatg acgagaccag ataatttcag cagtgcgttt gtaatagctg
     3001 ccggagcata atccagttcg ttaagcgttc ttttgtaata acccggtatg ctgtttgttg
     3061 aatggaagtt caagttgtag attgcgtcaa ctatctttct tggcaactta ccgcctttgg
     3121 taagtgccag tccattttgt gctattatag cattgataat gagtaaggat atctttacaa
     3181 ccggagattt atcagcgaca ttaattccgg ttgacgctcc aaacttaaca atgcattccg
     3241 gcgaaaatgg ccgatgaagt aaaatatgca tctgataggg agacaatccc tcaaagtcag
     3301 atttgggagc gttgttaaat cgatccacaa cattggctgc aattttattc agttcatcca
     3361 aagaggacgg ggcttcacct atccgatcta tttcagactg aatttgtttt aatatgtcat
     3421 cactgatttt agacatgacc agatcctttt taatattatt gatcacttac ttgaattact
     3481 cacttccctt ggcaacaaat cccagaattt cgatttcccg tttgcttaat tgtgatttct
     3541 tttcatgcct tgcctcagta aactgatgga ccctgcctgt tctctaatga taaaagactc
     3601 ataaaaatga tgaattatca ctatttcaca agagattaga gtattccatt tttgcataaa
     3661 caaatataat taaacaaatg gaaaccgaaa aaagattaaa aatgctgcaa ctattcgtaa
     3721 tatctcaagc aataagccat tttcaggact ttatgcacat ggtctttcca atgcattcat
     3781 ctcaattatg cctgcatcat ccatgcagga aaatgaattt caggcatcat actggttatg
     3841 agttacagtg acattaataa tcggcatgtt aatcaccctg ttacgttata aaaccttaat
     3901 ctgtcagggc aaaattgttc acagctatat tcctgttttc gtcattctcc tggtgatgat
     3961 atattaaggc attgtatcac agtattctga gtggcagaat ttgcaccttt caggtgtccg
     4021 gttatctttt ggacatgttc gtgaggcagc gcccggttct gaaagagccc gatatggata
     4081 acccacggtc catccggggg taagcagcat actatcactg ttcggctccc cgctctgcgg
     4141 gggactgaat gtaaggtgca ggggaagtat ctttcatcga ttgggatacc agactcctgc
     4201 cggagattcc tgtattcctc ttcaaatggc tttttaccgg caattcgatc cggaattaaa
     4261 cgaatcaaca ttccgttgga gattatatta cctaaaatca aaagggatta tcactcctat
     4321 atcccaggat cttttaacgc ttaattataa gccggttttt aaacctgatg tttctgatgt
     4381 agaagaaata gccagcaagg ccgagaagca attttccact atgaaatatt gcatctggtc
     4441 aacgaaagta atcagtgaat tcatgcttca tattccaggc aagtttatga ctgtcctgga
     4501 agtagagcaa gacgcattag agccattata tgaatatctg aagattcaga actacaataa
     4561 tgtttatatc ggaccggaag aaaaggaaat tgaacgttac attttcgaat cggaacaacc
     4621 gataatttta cagtcattaa tctcaaaaac tccaatccaa aaagttggta agatctatac
     4681 agttaccctt gaaaagctaa tcatagatct gttttgtgat aagcatttgt tcatcgcctt
     4741 ccgatatata ctttcaaaga ggatgaaagt atttgtattg taagacttac taatgttcat
     4801 attgaaagaa gttatcttta ttgcagttta tttttctttt ccagaaacaa aattattacg
     4861 gtgcgtcaga cgatgttgaa gggtgcacct gtactttgga gcctctctga taataaagag
     4921 tttgatgaat taatgtcatt caggttgtgg catgatgcta ataaggggca agaacttctg
     4981 gagtttgatt tttgatttct accccggctc tcattaaaaa tgttacgatt ttggtagtaa
     5041 tactacccgg attattaaat ttttaatgtt ttggatattt aagtccctga atttccaaat
     5101 tctgatcatt aatatgcttt atgatcccag ggaaatattc tccttctgga caatgaactt
     5161 tatcgggtat aatatggtga tcacaagtat gttatatacc cgtttgggta tatttatatt
     5221 taattatttg gtttttatac ccgaaaaggt atagatatat taattattat tatctttata
     5281 cccgaaaagg tataataaga tggaacagat tccaatgaca ttgtccgact ttcttaaaaa
     5341 caaaagaaag caattaaatc tgactcaaca acaattggcc gaaaaagctg gtgtcggatt
     5401 gcggtttatc cgtgaccttg agcagggtaa aacaacatta agaatggata aagtaaatca
     5461 ggtattaaag ctgtttgggc aggaacttgg tccacaacca ataaacagag aaaaactttt
     5521 aagaaaa
//
LOCUS       DAOSPX010000215         5526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13444_length_5526_cov_3.785414, whole genome shotgun sequence.
ACCESSION   DAOSPX010000215 DAOSPX010000000
VERSION     DAOSPX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13444_length_5526_cov_3.785414"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..672
                     /locus_tag="PLZ75_10095"
     CDS             178..672
                     /locus_tag="PLZ75_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOS72781.1"
                     /translation="MLKKNVEDLCNRQVEREGYSSILYLSMASWAEANGMAGIAEWLY
                     AQAEEEKDHMLRFVRYINERGGKAVIPAFSKPPEDFAGIVELFDDVLKHEQYITASIN
                     EIVELTLAEKDYSTHNFLQWFVAEQVEEESSAQAIVDKLKLGGRNILYQLDRDIMKLR
                     ESED"
     gene            921..4244
                     /gene="secA"
                     /locus_tag="PLZ75_10100"
     CDS             921..4244
                     /gene="secA"
                     /locus_tag="PLZ75_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOS72782.1"
                     /translation="MSIVNKVLGLFLGNKYERDLKELSPYVEKIHIEFEKLQGLSNDQ
                     LRDRSDAIRNRIKDHVKTDENEIESLREQAEREEDVYRKEELYDEIDRIEKRITEKLE
                     VFLDECLPEAFAIVKETAKRFKENSVLEVTAREYDRNLAATRESIVVKGDKAYWNNRW
                     IAGGNEITWDMVHYDVQLIGGVALHKGKIAEMATGEGKTVVATLPVFLNALAGRGVHI
                     VTVNDYLSKRDSEWMGPIYEFHGLTVDCIDKHQPNSAERRNAYNADITFGTNNEFGFD
                     YLRDNMAINPEDLVQRKHHYAIVDEVDSVLIDDARTPLIISGPTAKSDNLQFDELKPK
                     VETLVSAQKKLVTQILADAKKLISSEKEEDGGMLLLRAFKGLPKNNALIKFLSEEGNR
                     SLLQKTENFYMQNNSKEMPKVTDALYFIIDEKVNTIELTEKGIDLISTSVEDSSFFIL
                     PDVGSKIADLEKSDLDEREKLKKKDELLQDYSVKSERVHTMTQLLKAYTLFDRDIEYV
                     VMDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKENVKVEASTQTYATITLQNYFRMYH
                     KLAGMTGTAETEAGEFWNIYKLDVVVIPTNKPVIRADREDIIYKTKREKYNAVIDEII
                     SLNRQGRPVLVGTTSVEISELLSRMLKMKGLKHNVLNAKLHQREAEIVAEAGKTGTIT
                     IATNMAGRGTDIKLTDEVKKAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGSSQFFV
                     SLEDELMRLFGSERIAGIMDKLGLKDGEMIQHPMITRSIERAQKKVEENNFGIRKRLL
                     EYDDVMNSQREVIYKKRRHALLGERLSVDVANMMYDVTEHLVESYQEDGDFEGFDFDL
                     IRSFSLDSPVSSQEFLKTEAGVVIDKVYAKVLDTYKRKEELISRQAYPVLKDVYDRMA
                     HVYENVVVPISDGIRVFQVVTNLKATAESEGKELSKAYEKTVVLATIDDAWKEHLREM
                     DELKQSVQAATYEQKDPLLIYKFESFELFKAMINKVNKDVVSTLMKGHIPTQDPDQVK
                     EARQQRRQVDNLRASKSDFSQYGSGPGGGPDRDQKTEPVRVEKKVGRNDPCPCGSGKK
                     YKHCHGQQQAVAPGQSARI"
     gene            complement(4279..5157)
                     /gene="dapA"
                     /locus_tag="PLZ75_10105"
     CDS             complement(4279..5157)
                     /gene="dapA"
                     /locus_tag="PLZ75_10105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOS72783.1"
                     /translation="MKKFKGTGVAIVTPFKNDSSIDFSALGRVIDHVIGGGVNYIVAM
                     GTTGEASTLTKDEKQAVLSFVTESIDGRVPLVAGIGGNNTQEIINSLKELDLTGVDGI
                     LSVAPYYNKPGQRGLFQHFKEIATCSPLPVIIYNVPGRTSCNISSDTTLSLAHECDNI
                     VGIKEASGDMAQIMRIIRGKPENFLVISGDDLFTLPIIAAGGAGVISVLANAWPAQWS
                     EMVNHALKNNYRQAREIQFRFLEIIDLLFADGNPSGIKAFLSVLNLCRNNLRLPLVPV
                     SKNIMTRIQKAAESLR"
     gene            complement(5164..>5526)
                     /locus_tag="PLZ75_10110"
     CDS             complement(5164..>5526)
                     /locus_tag="PLZ75_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72784.1"
                     /translation="FYSGKDLPVNLTAVKFLSCIRTPELDFFYKPAYSVEAATFIKTR
                     FDVLIDINFDRKFPLYYVSTLSAAGLKVGLWDPGIRNPIFDVMIELKRPVRIDNYLEH
                     VMHYLEMMKSKSPEQVEK"
BASE COUNT         1654 a         1125 c         1387 g         1360 t
ORIGIN      
        1 cctataccga tgagcagaaa tataattatc tgaaaaacaa atatcctgct atgaaagatt
       61 tcatcagggc attcaatctt gatttcacct agtgatctta ttcaggaata actgaacctt
      121 gttgaacagt atccgcagat tgattgttaa tgttaaaatt ttaaaatgaa ttgaaatatg
      181 ttaaagaaaa atgtagaaga tctttgtaac agacaggttg aaagggaagg ctattcgtcg
      241 atactttatc tttcaatggc ctcatgggcc gaagcaaatg gaatggcagg cattgccgaa
      301 tggctttatg cccaggctga agaagaaaaa gatcatatgc tcaggtttgt aaggtatatt
      361 aatgaacgtg gaggcaaggc cgttattccc gcattcagta aaccaccgga agatttcgcg
      421 ggtatagtgg aactttttga tgatgtgctt aaacatgaac agtacatcac ggccagtatt
      481 aatgagatag ttgaactgac cctggctgag aaggattaca gtacccacaa tttccttcag
      541 tggtttgtcg ctgaacaggt ggaagaagaa tcatctgcac aggcaatcgt ggataagctt
      601 aagctgggtg gcaggaatat cctttatcag cttgacaggg atattatgaa actcagagaa
      661 tcagaagatt aactgattcc ggatcttatt ggaggattat ccgggtcagc agggatggcc
      721 cggaattatc tctatttaac ctgttggcgg cccttcaggc atcattacac tgatgcttcc
      781 gtcatttctg taaccgtacc acccgcggtg agtctgaata cagccccgcc gcccgtaaaa
      841 ccggaatgga attcaaatcc attatcggaa aaatagatta attttgcagt atattttgaa
      901 aatctgtaag gctgttaaaa atgagcattg taaataaagt tctggggctt tttctgggga
      961 ataagtacga gagggatctg aaggagttaa gtccttatgt tgaaaagata catattgaat
     1021 ttgaaaaact tcagggtctg tcaaatgatc agcttcgtga caggagtgat gcaataagaa
     1081 atcggataaa ggatcatgta aagacggacg agaatgagat agaatctctc agggagcagg
     1141 ctgaaaggga agaggatgtg tacaggaagg aagaattgta tgatgagatt gacaggatcg
     1201 agaaacgcat cactgaaaag ctggaagtct tccttgatga atgcctgcct gaggcttttg
     1261 ccatagtaaa ggaaactgca aaaagattca aggagaacag tgtgcttgaa gttacggcaa
     1321 gggagtatga ccggaatctg gccgccacgc gggaatcgat tgtcgtgaag ggtgataagg
     1381 catactggaa caacaggtgg attgccgggg gcaatgagat aacatgggat atggttcatt
     1441 atgatgtgca gcttatcgga ggggtggcgc tgcataaggg aaagattgcc gagatggcca
     1501 ccggggaggg aaaaaccgtt gtggctaccc tgcctgtgtt ccttaatgcc cttgcgggaa
     1561 gaggagtgca catagtgacc gtaaatgatt atctctcaaa gagggactcc gaatggatgg
     1621 gtcccatcta tgaattccac gggcttacgg ttgactgtat agataagcac cagcccaatt
     1681 cggctgaaag gcggaatgcc tacaatgcgg atatcacctt cggtacaaac aacgaattcg
     1741 gattcgacta cctgagggat aacatggcaa tcaacccgga agaccttgtt cagagaaagc
     1801 accattacgc aattgttgac gaggttgact cagtccttat tgatgatgcc agaacgcctc
     1861 tgataatttc aggtcctact gcaaaaagtg ataacctgca gttcgatgag cttaaaccaa
     1921 aggtcgaaac acttgtcagc gcacagaaaa aactggttac ccagatcctt gccgatgcaa
     1981 aaaaactgat ctcttctgaa aaggaagaag acggaggcat gctgctactg cgggcattca
     2041 agggactgcc caagaacaat gccctgataa aattcctcag tgaggaagga aacaggtctc
     2101 ttctgcagaa aacagaaaat ttctacatgc agaacaacag caaggaaatg ccaaaggtga
     2161 ctgatgctct ttattttatt attgatgaga aagtcaatac gatagaattg actgaaaaag
     2221 gcattgatct tatttcaact tcagtcgagg attcatcctt cttcatcctt ccggatgtgg
     2281 gatcaaaaat tgcagatctc gagaaatcag atctggatga gagggaaaaa ctgaaaaaaa
     2341 aggatgagct gcttcaggac tattccgtca agtcggaaag ggttcacacc atgacacagc
     2401 ttctgaaggc ctatactctc tttgacaggg atattgaata tgtggtcatg gacaacaagg
     2461 taaagatcgt ggatgagcag acagggagga tactggaggg aaggcgctat tccgacggcc
     2521 tgcaccaggc tattgaagcc aaggaaaacg taaaggttga ggcatcaacc cagacctatg
     2581 ccaccattac tctccagaac tatttcagaa tgtatcacaa gcttgccggt atgacgggta
     2641 cggcagaaac ggaggccgga gaattctgga atatctacaa gcttgatgtg gtggttatcc
     2701 ccacaaacaa accggtcatc cgggcagacc gtgaggatat aatctacaag accaaaaggg
     2761 aaaaatacaa tgcggttatt gatgagataa tttctcttaa ccgccaggga aggcccgtcc
     2821 tggtcggaac aacatctgtt gaaatatccg aattgctgag cagaatgctc aagatgaaag
     2881 gactgaagca taatgttctt aatgcaaagc ttcatcagcg tgaggcagag atagtagcag
     2941 aggcaggcaa gaccggaacc ataaccattg ccaccaatat ggccggaagg ggtacggata
     3001 tcaagctgac cgatgaggtg aaaaaggcgg gaggcctggc aatcataggt acggaaaggc
     3061 atgagtcgag aagggttgac aggcagcttc gcggacgttc gggaagacag ggcgacccgg
     3121 gatcgtcaca gttctttgtt tccctggagg atgaactgat gaggctcttc ggttccgaaa
     3181 ggattgccgg tataatggat aagctcggac tcaaggacgg ggaaatgatt cagcatccca
     3241 tgattaccag gtcaatcgag agggcacaga aaaaagtgga ggaaaacaac tttggcatcc
     3301 gcaaaaggct tcttgaatat gacgatgtta tgaactccca gcgggaggtg atttataaaa
     3361 aaagaaggca tgcgctgctg ggggagaggc tcagcgtgga tgtcgccaac atgatgtatg
     3421 acgttaccga gcatctggtg gagtcatatc aggaggatgg agattttgaa ggctttgatt
     3481 ttgatcttat ccgttcattc tcactagatt ctcctgtttc atcccaggag tttctaaaaa
     3541 ctgaagcagg ggtggtgatc gacaaggtat atgccaaggt tctcgataca tacaagcgaa
     3601 aggaagaact gatttcgcgc caggcctatc ccgtgctgaa ggacgtctat gaccggatgg
     3661 cacacgtgta tgagaacgtg gttgtaccga tctcggatgg catcagggtg ttccaggtgg
     3721 taaccaatct taaggcaaca gccgaatcgg aggggaagga attatcaaaa gcctatgaaa
     3781 agaccgtcgt actggctaca attgatgatg cctggaagga gcacctgcgt gagatggatg
     3841 aactgaagca gtccgtacag gctgcaacct atgagcagaa agatccgctg cttatataca
     3901 agttcgaatc ctttgagctt ttcaaagcca tgataaacaa ggtgaacaag gatgttgtca
     3961 gtactctcat gaaaggccat atacccactc aggatcctga tcaggtaaaa gaagccaggc
     4021 agcagaggcg gcaggtggat aatctcaggg catcaaaaag tgatttttca caatatggtt
     4081 ccggacccgg tggcggaccg gacagagacc agaaaacaga accggtaagg gttgaaaaga
     4141 aagtaggaag aaacgatccc tgtccctgcg gcagcggcaa aaagtacaaa cactgccatg
     4201 gtcagcagca ggcagttgct cccggtcaaa gtgcaaggat ataaatgaaa tgaaagtaag
     4261 cagctgactg aaggataatc agcgcaggct ttccgcggct ttctgtatgc gtgtcataat
     4321 atttttgctg accggcacaa gcgggagcct taaattattc ctgcacaggt tcaatactga
     4381 cagaaatgcc tttatacctg aagggtttcc gtcggcaaac agcaaatcaa taatttccag
     4441 aaatctgaat tgtatttccc gggcctgcct gtaattgttt ttcagtgcat gattaaccat
     4501 ttcactccac tgtgccggcc aggcattggc aagaactgag attaccccgg caccgccggc
     4561 tgctatgatt ggcagggtaa acagatcatc gcccgagatt acaaggaaat tttccggttt
     4621 tcccctgatt attctcatta tctgtgccat gtcgcctgat gcttccttta tgccgactat
     4681 attgtcgcat tcatgtgcca ggctgagagt ggtatccgat gatatgttgc agctggtcct
     4741 gccgggaacg ttgtatatta tcacaggcag cggactgcag gtggcaattt ccttgaaatg
     4801 ctgaaacaat cctctctgcc ccggcttgtt gtaatatggc gccactgaca gtatcccgtc
     4861 aactcccgtg agatcaagct ccttcagact attgattatt tcctgtgtat tgtttccccc
     4921 tattcctgca acaagaggta ccctcccgtc tatggactcc gtaacaaacg acaggactgc
     4981 ctgtttctca tcctttgtca gggttgaggc ttcacctgtt gttcccatgg caacaatgta
     5041 attgacacca ccgcctataa cgtgatctat tacacgcccc agcgcggaga aatcaatgct
     5101 gctgtcattt ttgaagggag ttacgattgc aacgccggta cctttgaatt tcttcataat
     5161 atattatttt tcaacttgtt caggtgattt tgatttcatc atttcaagat aatgcataac
     5221 gtgttcaaga tagttatcta tcctgaccgg ccttttcagt tcaatcatga catcaaatat
     5281 cggattcctt atccccggat cccataatcc gacctttaaa cccgctgcag acagggtgga
     5341 gacatagtaa agaggaaatt tcctgtcgaa attgatatca ataagaacgt caaaccttgt
     5401 ttttatgaat gtggctgctt caactgaata ggcaggcttg taaaagaaat caagttccgg
     5461 tgtcctgatg catgacagaa acttgacggc agtgagattt acagggagat ccttgcctga
     5521 ataaaa
//
LOCUS       DAOSPX010000216         5525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13449_length_5525_cov_4.516088, whole genome shotgun sequence.
ACCESSION   DAOSPX010000216 DAOSPX010000000
VERSION     DAOSPX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13449_length_5525_cov_4.516088"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLZ75_10115"
     CDS             complement(<1..293)
                     /locus_tag="PLZ75_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS72785.1"
                     /translation="MKKRIIVTGGTGYIGSHTAVELIQEGYEVILLDNLYNSEAEVAG
                     RIGQITGTTPVLEVIDLCDASKLDATLKKHLGADAIIHFAAYKAVGESVNKPL"
     gene            complement(495..1448)
                     /locus_tag="PLZ75_10120"
     CDS             complement(495..1448)
                     /locus_tag="PLZ75_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72786.1"
                     /translation="MNPEPKNFGLIGVAGYIAVRHLNAIKETGNNLLASLDPFDSVGL
                     LDSYFPQSDFFVEFERFDRHFDKLKRSGTRIDYVSICSPNYLHDSHIRFALRQGADAI
                     CEKPIVLNPWNVDALQIIENETGRKINTVLQLRLHPRIVELKQMIENGPKDKVYDVDL
                     TYITSRGNWYFRSWKGDIEKSGGVSTNIGIHFFDMLGWIFGEVKNNVVNISEYNKAAG
                     YLELKNARVRWFLSLDFNDIPGEYKENGKRTFRSVTVEGEEVEFSEGFTDLHTASYRE
                     IIEGRGFGLNDARQSVVTAHTIRNAKPIGLQGNYHPMLRRI"
     gene            complement(1505..2098)
                     /locus_tag="PLZ75_10125"
     CDS             complement(1505..2098)
                     /locus_tag="PLZ75_10125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOS72787.1"
                     /translation="MKDMDKKFFAHETAVIDEGCKIGAGTKIWHFSHVMTGAEIGENC
                     NLGQNVVVSPGVRLGKNVKVQNNVSIYTGVICDDDVFLGPSMVFTNIVNPRSRVVRKD
                     SYVTTIVERGASIGANATIVCGNIIGRYAFIGAGAVVTRDVRPYALVVGNPARQTGWM
                     SEYGHRLNFDSKGFAVCPESKERYRLENNTVTKISKD"
     gene            complement(2098..3228)
                     /locus_tag="PLZ75_10130"
     CDS             complement(2098..3228)
                     /locus_tag="PLZ75_10130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS72788.1"
                     /translation="MRKIEMVDLKSQYRKLSGEIDDAIRSVLGSTAFIKGPEVKLFEE
                     ELREYLGAGHVVSCGNGTDALQIAMMALDLKPGDEIITTNFTFISTVEVVALLGLKLV
                     LVEPDPCTYNISPAAVERAVTSRTRAIVPVHLFGQCADMESLTEIAAEHGLYIIEDAA
                     QATGTDYIFSDGTRKKAGTIGTIGTTSFFPSKNLGCYGDGGAMYTNDPGLAKKIRSIA
                     NHGMKIKYHHDDIGVNSRLDTIQAAILNVKLKYLDEFNNARKAAADYYDEAFSGCKSL
                     ITPERSSFSTHIFHQYTVRVTDGRRDELRNYLAGKDVPSMVYYPGPLHSQNAYRYLGY
                     GVNDFPVTSELCREVLSLPMHPDLEQDQLDHIAISVLEYLNK"
     gene            3524..4672
                     /locus_tag="PLZ75_10135"
     CDS             3524..4672
                     /locus_tag="PLZ75_10135"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOS72789.1"
                     /translation="MDITPFICELLFSHDCVIVPGFGGFVGNYASARIDRATFTFYPP
                     AKQISFNRNLRNNDGLLAGRISEQLQISFSDSVNLIEEFVTDIRTRIDRKEKVVFENI
                     GLFSVNHEGSIQFEPQGNINYHRSSYGLESFQFSPAEAYDVRKRVTGHSVRDTDRRHD
                     IRTFLWKAAVIVPLLAAATIIPLKTSLFRPRLETTTLNPIVKSASENTGRPLAGAEIN
                     NAAADNRENIADAGGDAGILPSDTKSGLPAGDVQVNIPVAVAETHTSAADSEISVPAA
                     DASTDAEALRPFPGDRGKHSGGIISGKAPAPGTEGQYCLITGSFRLRENADQQAEALR
                     REGFSPEIISSINGFFRVSAMRCRDLPAAIEKQKDLDEKYPGIWIRKI"
     gene            complement(4675..>5525)
                     /gene="metG"
                     /locus_tag="PLZ75_10140"
     CDS             complement(4675..>5525)
                     /gene="metG"
                     /locus_tag="PLZ75_10140"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF039287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOS72790.1"
                     /translation="LVLTINYYEGRVPARTVILPEDEEVLREIGQIALNVENAIEAFR
                     FREALKEAMNLARLGNKYLAEAEPWKIVKTDPERVKTIMNIALQITANLAIVLEPFLP
                     FSMEKLRGWINTGKPAWDMAGNPDILPEGHLINKPGLLFEKIEDDAIKRQVEKLHAGK
                     QADDDTAEEIPQGKETVSFEEFSRIDIRTATVLEAEKVPKTSKLLRLKIDTGIDIRTI
                     VSGIAEYFEPQSLVGRQISIVANLEPRKIRGIESKGMILMAEDRDGKLVLVSPLEKVN
                     NGSVIK"
BASE COUNT         1270 a         1384 c         1234 g         1637 t
ORIGIN      
        1 tcaaggggct tgtttactga ttcacccaca gccttgtagg ctgcaaaatg aataatggca
       61 tcagccccta agtgtttttt cagtgtggca tcaagctttg aagcatcaca aaggtcaata
      121 acttccagca caggcgttgt gccggttatc tgccctatcc tgccggcaac ttccgcttcc
      181 gaattgtaaa gattatccag gagaatgact tcatagcctt cctgtatgag ttccacggct
      241 gtatgtgagc caatgtagcc tgttcctccc gtaacgatta ttcttttttt catctgtcta
      301 tatttttaat ttaatggtca gatacttgtc ccggtttatc ggaaacctgt tccgatttat
      361 cggagcctgt cccgatttat cgggaacctg ttccgattta tcggaaaccc atgaatctga
      421 acccacatcc ctgtttaaat tcctaacatg gccgtttcat tgtcatgacg cttaattgtc
      481 agtatgcagt catctcagat tctcctgagc atgggatgat aattgccctg aaggcctatt
      541 ggtttggcat tcctgattgt atgcgcggtt acaactgact gcctggcatc attcagtccg
      601 aatccgcgcc cttcaattat ttccctgtag cttgccgtat gaaggtcggt gaatccttca
      661 ctgaattcca cttcttcgcc ctcaacggtc accgatctga aggtcctctt tccgttttcc
      721 ttgtattccc ctggtatgtc attgaaatcc aggctgagga accatcttac cctggcattt
      781 ttcagttcaa ggtagccggc tgccttgttg tattccgata tgttgacgac attgtttttc
      841 acttccccga atatccagcc gagcatgtca aagaaatgta ttccgatgtt tgtgcttacc
      901 ccgcctgatt tctcaatatc tcccttccat gacctgaagt accagtttcc ccggctggtg
      961 atataggtga ggtcaacatc gtataccttg tctttcggac cgttctcaat catctgcttc
     1021 agttccacga tcctcggatg cagcctgagc tgcaggacag tgttgatctt ccggccggtt
     1081 tcattttcta ttatctgcag ggcatcaaca ttccacggat tcagaacgat gggcttttca
     1141 catattgcat cagcgccctg cctgagtgca aacctgatat gcgaatcgtg caggtagttc
     1201 ggggaacaga tactgacgta atctatcctt gtcccggatc ttttcagctt gtcgaaatgc
     1261 ctgtcgaagc gttcaaattc aacgaaaaaa tcactttgcg ggaaatagct gtcgagaaga
     1321 cccacactgt caaaaggatc aagggatgcc aggaggttgt tacccgtttc cttgatggca
     1381 ttcagatgcc tgactgcaat atatcctgcc actcctatca ggccgaaatt tttgggttcg
     1441 ggattcatga tatctgtttg cttaatattt tactctttgc ttatttttac tccccggata
     1501 tttttcagtc cttgcttatt tttgtaacgg tattgttttc cagacggtat ctttccttgc
     1561 tttcggggca caccgcaaag ccctttgaat cgaaattcag cctgtgcccg tattcgctca
     1621 tccatcctgt ctgccttgca ggattaccca ccacgagggc atagggcctg acgtccctgg
     1681 tcaccacggc tcctgcacct ataaaggcat atcttccgat aatgttgccg catacaatgg
     1741 tggcattggc tccgatggaa gctccccttt caacgattgt ggttacgtag gaatcctttc
     1801 tgaccacacg gctgcgggga ttcacaatat ttgtaaacac cattgaggga ccgagaaaaa
     1861 catcatcatc acagataacc ccggtgtata tcgatacgtt gttctgtacc tttacatttt
     1921 tccccagtct tactcccggg gaaacaacca cattctgccc gagattgcag ttttcaccta
     1981 tttcagcacc tgtcatcaca tggctgaaat gccatatttt cgttccggcg cctatcttgc
     2041 agccttcatc aataactgct gtctcgtgcg caaagaattt cttatccata tctttcatta
     2101 cttattcagg tattccagaa cgcttattgc aatatgatcg agctgatcct gctcaagatc
     2161 gggatgcatg ggcaatgaca gaacttccct gcagagctcc gatgttaccg ggaagtcatt
     2221 taccccgtat ccaagatagc ggtaggcatt ctgtgaatgc agtggtccgg ggtaatacac
     2281 cattgacggg acatcctttc cggcaagata gttcctgagc tcatccctcc ggccgtcagt
     2341 gacccttacc gtgtactggt gaaatatgtg ggtcgagaaa ctgctccgct cgggtgtgat
     2401 gagggatttg catcccgaaa aggcctcgtc gtaataatct gcggctgctt tcctggcatt
     2461 attgaactca tcgaggtatt tcagctttac attcagaatt gctgcctgaa tggtgtcaag
     2521 ccttgaattc acgcctatat cgtcatgatg atattttatc ttcattccgt gattggctat
     2581 gcttcttatt ttttttgcca gtccgggatc attggtatac attgccccgc cgtcaccgta
     2641 gcagcccaga ttcttggaag ggaaaaatga agttgttcct atggttccta ttgttccggc
     2701 ctttttcctc gtaccgtcag aaaaaatata atctgttccg gttgcctgcg ccgcatcttc
     2761 aataatatac agaccatgtt ccgctgcaat ttccgtgagg ctttccatgt cggcacactg
     2821 gccgaaaagg tgcaccggaa ctattgccct ggtccgggaa gtgacggctc tttccacagc
     2881 tgcgggtgat atattgtagg tacagggatc gggttcaaca agaacaagct tcagtccgag
     2941 cagggcaaca acctcaactg tggaaatgaa ggtgaaattc gttgttatga tttcatcccc
     3001 cggcttcagg tcaagcgcca tcattgcaat ctgaagcgca tcggtcccgt tgccgcagga
     3061 aacaacatgg ccggcaccga gatattccct cagctcctct tcgaatagtt ttacttccgg
     3121 cccttttatg aatgccgttg acccgaggac ggatcttatc gcatcatcaa tctctcccga
     3181 cagtttcctg tactggcttt tgagatcgac catttcaatt ttccgcatac tgcctgtgat
     3241 tatccgaaag gcgaagataa ttattttcgg ttaactcccg tgtgctgagt tttcaacaag
     3301 gtttattccc gccggatgtt ccggagaagc cggctcaaaa ggcctgtaat gtaaaccttg
     3361 tttccgttca ggctgcttca attcaaagta tccgttcaga aagctgcgat tcactgtatt
     3421 cccgtatgcc tgtacaggcc tgcagttttt tcttatttat gtccggattt cagtttgatt
     3481 gttgagaatt ttcttatttt tgtaatggaa gttaaaatct gaaatggaca ttactccttt
     3541 tatatgcgaa ctgttattca gtcatgactg cgtcattgta ccgggtttcg gaggctttgt
     3601 cgggaattat gcctcagcac gcattgacag ggccacattc actttttatc cgccggcaaa
     3661 gcagatttcc tttaacagga atctcaggaa caatgatggg cttcttgccg gaaggatttc
     3721 cgagcagctt caaatatcct tcagtgattc agtaaatttg attgaggagt ttgtaacgga
     3781 tatcaggacg cggattgacc ggaaggaaaa agttgttttt gaaaacatcg gtttgttttc
     3841 ggttaaccat gaaggaagta ttcagtttga acctcagggt aatatcaatt atcaccggtc
     3901 ctcctacgga cttgaatcct ttcagttttc accggctgag gcctatgatg tgagaaaacg
     3961 ggtgacgggt cattccgtta gggatactga cagaaggcat gatatccgta ctttcctgtg
     4021 gaaggctgcg gtgattgttc cccttcttgc tgcagcgacc atcataccgc tgaagaccag
     4081 ccttttcaga cccaggctgg agacaacaac ccttaatccc attgtaaaat ccgcatctga
     4141 aaataccggc agacctctgg caggagcaga aataaacaat gcggctgctg ataaccggga
     4201 aaatattgct gatgcaggag gtgatgcagg cattctgcct tcagatacca aatccggtct
     4261 tccggccgga gatgttcagg taaatattcc ggttgccgtt gctgaaacac atacctctgc
     4321 agcggattca gaaattagcg ttccggcagc ggatgcttca acagatgctg aagccctgcg
     4381 gccttttccc ggcgaccgag ggaaacattc cggtggaatt atcagtggca aagctcccgc
     4441 accgggaacg gaagggcagt actgcctcat aaccggcagt ttcagactga gggaaaatgc
     4501 tgatcagcag gcagaggccc tgagaaggga aggcttcagt ccggaaatca tttcatccat
     4561 taacggattc ttcagggtca gtgccatgag atgcagggat cttcctgcgg ctattgagaa
     4621 acaaaaggat ctggacgaaa aatatcccgg tatctggatc aggaaaatct gagactattt
     4681 tatcacactt ccgttattta ccttttcgag aggggagaca agcaccagtt tgccgtccct
     4741 gtcctcggcc atcaggatca tgccctttga ttctataccc ctgattttcc tgggctcgag
     4801 atttgccact attgatatct gtctgcccac cagtgactga ggttcgaaat attctgctat
     4861 tcccgacaca atggtgcgta tgtcaatacc cgtatcgatt ttcagccgga gcagcttgct
     4921 tgtcttgggt accttctcag cttccaggac agtggcagtc cgtatatcaa tccttgagaa
     4981 ttcctcgaag gaaaccgttt cctttccctg cgggatctct tctgcagtat catcatccgc
     5041 ctgtttgccg gcatgaagct tttccacctg cctttttatg gcatcatcct caattttctc
     5101 gaaaagcagt cccggtttgt tgatgagatg gccttcaggc aggatgtcag gattcccggc
     5161 catatcccag gcaggtttgc ccgtatttat ccagcccctc agtttttcca tggaaaaggg
     5221 caggaagggc tcgagcacta ttgccaggtt tgcagttatc tgcagtgcaa tgttcataat
     5281 ggttttgacc ctttccggat cggttttgac tatcttccat ggttcggctt ccgccagata
     5341 cttgtttccc agccgggcca ggttcatggc ttctttcagt gcttccctga acctgaatgc
     5401 ttcaatggcg ttttcaacat ttagcgctat ctggccgatc tctctcagaa cctcctcgtc
     5461 ttcaggcagt atcactgttc ttgcaggcac cctcccttca taataattaa ttgtaaggac
     5521 aaggg
//
LOCUS       DAOSPX010000217         5502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13520_length_5502_cov_4.131632, whole genome shotgun sequence.
ACCESSION   DAOSPX010000217 DAOSPX010000000
VERSION     DAOSPX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13520_length_5502_cov_4.131632"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /locus_tag="PLZ75_10145"
     CDS             <1..1983
                     /locus_tag="PLZ75_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72791.1"
                     /translation="GGYELITGVFLGSWLTASAAGSVLAGRSRFSNLRTISLLFSLSP
                     LLSLLLLVTLTRIMTGPGETPDFLKSLVITFIMLMPFCLASGYIFIKLLSAARTRDDF
                     VPGKSFSIETTGGILAGIILSVLTSGLLNTGQILVVILVSAAAWAVLSYFPAGSLSDY
                     LIKSLFAVIAAAALMAGPGTVLRQLMMPGIRVSVTKDTPYGNITKGLYGGEVSMYYNQ
                     RLLSYSEDAVEREENIHYALLPAKNPQKILLVSGNLASSLEELAKYSPEKVIYVENDP
                     GLIEMTQVNTDAYSFSIGIEMRDAFRYIRETGEKFDVAIVLLPPPSTLALNRYYTSEF
                     FAQIRDRLNPGGIFMCSPGSYDFYLNRESVLLYSSVFNSMREIFRYVRPVAGHKLHFI
                     SSDSAFPVSFSRMTEEKQISNIYVGPDFLYDDLIELKSDELLSVLDANVRQNRSASPI
                     ACFYYQSYNLSRYPHAKALAIAVSAAIFALSLLTIRRKNILMYFTASSLAGFEIIILL
                     ILQITIGNMYQFTGTVIASLMTGLAAGSGIRRIAAGPFPARTLSMVLIIYYILFALCV
                     RPLLTVTGTAGIILIMAAVFPPSFITGHLFRILTIHDSEGSLSSSVYSADLAGSAVGF
                     ILVSAIMVPVFGIVTTIIFLSGLILTGIIFGGNKIK"
     gene            2018..3154
                     /gene="amrS"
                     /locus_tag="PLZ75_10150"
     CDS             2018..3154
                     /gene="amrS"
                     /locus_tag="PLZ75_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOS72792.1"
                     /translation="MASNEFNRSKREFIRKCLALSAGVVAMPRFPLNAAPEAEVQGTA
                     GRLAMFQEETPRGVMCLLCPNGCILKDGEISRCNNRTVRKSRLYTMAFGNPCAINIDP
                     MEKKPLYHFHPGTRIFSIATAGCNLACLNCQNWSISQTRPDKTRNYDLMPERVVEEAL
                     KNNCRSIAYTYSEPTSFYEYMYETSVLAKRAGIKNVAVSNGYVNPEPLKKLCTVLDAA
                     NIDLKSFNDSTYMKLTGGKLQPVLDSLLIYRDSGVWLEITCLIVPTWTDKADEIAAMC
                     KWLSEGGFRDTPVHFTRFYPIYKLDQLPPTPVEFLNEAAGIARQEGMKYIYTGNVPGS
                     EQANTICPGCGTRLILRQGFRIVSNDIRKGRCSKCGREIHGVWS"
     gene            3189..5264
                     /gene="ligA"
                     /locus_tag="PLZ75_10155"
     CDS             3189..5264
                     /gene="ligA"
                     /locus_tag="PLZ75_10155"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOS72793.1"
                     /translation="MDRTEAGERIEKLRREIETHNHSYYVLSNPLISDYEFDQLMNEL
                     EALEKEYPDLITPDSPTQRVGSDLTPEFRQYEHKYPMLSLGNIYNEEELKAFCDRIEK
                     TAGGPVEYVCELKFDGVSVSLNYRNNSLYMALTRGDGNAGDDVTRNVRTIKSIPLRVS
                     GSNIPPDFVIRGEILMSRPVFDRLNRERIQDGLPAFANPRNAASGTLKLLDPKIVASR
                     RLDCMFYFLLGEDIPHETHYKNLKQAEEWGFRISEGISICRNAAEIMDFVKKWDEDRK
                     KLIYDIDGVVIKVNSLALQQEAGFTAKSPRWAAAFKFRAEQVTTRLLSVSFQVGRTGT
                     VTPVANLDPVFLAGSTVRRASLHNADQIGILDLHINDMVYVEKGGEIIPKITGVDLSE
                     RDENNIKVSFITRCPECGTPLERNEGEANHFCPNYLHCPPQITGRIEHFISRRAMAIE
                     GLGTETVDLLFKEGLIRNFADLYELRTEQLVPLERLGEKSARNIINSIRESAGIPYSR
                     VIFALGIRHVGETTARTIARNFRSVDELMNADFERLTGIREIGPRIASSIISYFRDEE
                     NIEIIRRLKSYGLNLREERKEERKEEDQEEHETEEGRKEGMKEGKKEAVLAGKSIVIS
                     GVFHRHSRDEYREIIEKRGGKNVSSPSSNTSFILAGENMGPSKREKAESLGIPLISEE
                     EFLELIGEE"
     gene            5308..>5502
                     /locus_tag="PLZ75_10160"
     CDS             5308..>5502
                     /locus_tag="PLZ75_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72794.1"
                     /translation="MVLLGEFRQKVGVYILDRKGSRSKRKVHYSNIESVKKIGIVWDA
                     SNNEELAVLSQFHRQLNEKDI"
BASE COUNT         1576 a         1249 c         1356 g         1321 t
ORIGIN      
        1 ggcggatatg aacttatcac aggtgtcttt ctcggatcat ggcttacagc ttctgcagcc
       61 ggttccgtgc tggccggcag atcccgcttc agcaacctcc gcacgatcag ccttctcttt
      121 tccctgagcc ccctcctgtc gctgctgctc cttgtgacac tgacaaggat aatgaccgga
      181 cccggggaaa caccggactt tctgaaaagc cttgtcatta ctttcattat gctgatgccc
      241 ttctgcctgg catcaggata catattcata aaactgttat cggctgccag gacaagggat
      301 gattttgtac ccggaaaatc tttttcaata gagacaaccg gaggcattct cgcaggaatc
      361 attctttccg tcctgacatc cggtctgctg aataccggac agatactggt tgttattctc
      421 gtttccgcag cagcctgggc tgttctttca tattttcccg ccggaagcct ctcagactat
      481 ctgatcaaat ccctttttgc agtaattgcg gcggcagcac tgatggccgg acccggtacc
      541 gtgctgaggc agctgatgat gcccgggatc agggtttcag taacaaagga cacgccttac
      601 gggaatatta ccaaagggct atatggcgga gaggtaagca tgtattacaa ccagaggctt
      661 ctttcatatt ctgaagatgc cgtggaaagg gaggaaaata ttcactatgc ccttcttccg
      721 gcaaagaatc cgcaaaagat actgctggtt tcaggcaatc ttgcttcatc tttggaagag
      781 cttgcaaaat acagccctga aaaagtaatt tatgtcgaaa atgatcccgg tcttattgaa
      841 atgacacagg tcaatacaga tgcatacagt ttcagcatcg gtattgaaat gagggatgcc
      901 ttcagatata tcagggaaac aggagaaaag tttgatgtgg ccattgtcct gctgccccct
      961 ccatcaacac tggctctgaa cagatattac acaagtgagt tcttcgcaca gatcagggac
     1021 agactaaacc ctggcggcat tttcatgtgt tccccgggca gttatgactt ttacctgaac
     1081 agggaatctg tcctgcttta ctcatcggta ttcaacagca tgagggaaat tttcagatac
     1141 gtccgacccg ttgcaggaca caagcttcat tttatttctt ccgactcggc gttccctgta
     1201 tcgttttccc ggatgacgga agaaaaacaa atctccaata tctatgtggg acctgatttc
     1261 ctgtacgacg atcttattga gctaaaatcg gacgaactgc tgtcggtgct ggatgcaaat
     1321 gtcaggcaga accggtcagc ctcaccgata gcatgttttt attaccagtc atacaatctt
     1381 tcacgctatc ctcatgcgaa ggccctggcc atcgcggttt cagccgccat attcgcactg
     1441 tcgcttctga ccatacgcag gaaaaatatc ctgatgtact tcacggcatc ctcacttgca
     1501 ggttttgaga ttattatact gctgattctg caaattacca taggcaatat gtatcagttt
     1561 acgggcacag taattgcctc cctgatgacg ggacttgccg cgggttcggg aatacggcga
     1621 atagcggccg gtccttttcc ggccaggaca ctgtcaatgg tgctgataat ctattacatc
     1681 ctctttgcac tatgcgtcag gcccctgctg acagtaacag gcacggcagg aatcattctc
     1741 atcatggcag ctgtttttcc cccgtctttc ataacaggtc atctgttcag gattcttaca
     1801 atacatgaca gtgaaggatc gttatcatca tcggtataca gcgccgacct ggccggttcg
     1861 gctgtaggat tcatccttgt ctcggccata atggttccgg tttttggaat agtgacgaca
     1921 ataatttttc tttcgggact tatactaaca ggaatcattt ttggaggtaa caagatcaaa
     1981 taattgttat attacagcat tacacaaagg aggcgatatg gcgagcaatg aatttaacag
     2041 gagcaagaga gaatttatca ggaaatgcct ggccctgtcg gcaggtgtgg tggcgatgcc
     2101 ccgctttccg ctgaacgctg ctccggaagc ggaggtacag ggaacggcgg gaagactggc
     2161 aatgttccag gaagaaactc caaggggtgt aatgtgcctg ttatgtccca acggatgcat
     2221 tctgaaagat ggcgagataa gcaggtgcaa caaccgtact gtaaggaaat ccagacttta
     2281 tacaatggct ttcggcaatc cgtgtgccat caatatcgat ccgatggaaa agaaacccct
     2341 gtaccacttc catcccggta cgagaatctt ctccattgca actgccggct gcaaccttgc
     2401 ctgtctcaac tgccagaact ggtccatctc ccagaccagg cccgacaaaa ccaggaacta
     2461 tgatctcatg ccggaaagag tggttgagga agccctgaaa aacaactgcc gctccatagc
     2521 ctatacatat tcagaaccca cttcgtttta tgaatacatg tatgagacct cggtactggc
     2581 aaaaagagcg gggattaaaa atgttgccgt ttcaaacggt tatgtaaatc ccgaaccgct
     2641 gaaaaagcta tgcactgttc tggatgcggc caatatcgat ctcaagtcat tcaatgacag
     2701 cacatatatg aaactgacag gaggaaaact gcagcccgtt cttgattcgc ttctgattta
     2761 ccgcgattcc ggtgtatggc tggaaataac ctgtctgatc gtgcctacgt ggacagacaa
     2821 ggcagatgaa atagctgcaa tgtgcaaatg gctgagtgaa ggcggattca gggatactcc
     2881 cgttcatttc accaggttct atccgattta caagcttgac cagctccctc ccaccccggt
     2941 ggagtttctg aatgaggcag cagggattgc aaggcaggaa ggtatgaaat atatatacac
     3001 cggaaatgtt ccgggaagcg aacaggcaaa caccatttgc cccggatgcg gtacccgcct
     3061 gattctgagg cagggattca gaatcgtctc gaatgatatc aggaaaggca gatgcagcaa
     3121 atgcggaagg gaaattcacg gcgtctggtc gtaaaaataa aaacagactc tcaacagaaa
     3181 cacacaatat ggaccggaca gaagcaggag aaaggataga aaaactgcga agggaaattg
     3241 aaactcacaa tcacagttat tatgtattga gcaatccgct gatttccgac tatgagttcg
     3301 atcagctgat gaatgaactt gaagcccttg aaaaggaata tcccgatctc attacccccg
     3361 attcccccac ccagagggtg ggcagcgacc tcacaccgga attcaggcag tatgaacata
     3421 aatatcccat gctttccctg gggaatattt ataatgagga agaactgaag gcattttgcg
     3481 acaggattga gaaaacggca ggaggaccgg ttgaatatgt ctgcgagctt aaatttgacg
     3541 gggtatcggt aagtctgaac tacaggaata acagcctgta catggccctg acccggggtg
     3601 acggcaacgc gggggatgat gtcaccagga atgtcagaac catcaagagc attcctctca
     3661 gggtttcagg cagcaatatt cctcccgact ttgtgattag gggtgagata ctgatgtcca
     3721 gaccagtttt tgacagactg aacagggaaa ggatacagga cggcctgccg gcttttgcca
     3781 atcccaggaa tgcagcttcc ggcactctta agcttcttga ccccaagatt gttgcctcac
     3841 gccgtcttga ctgcatgttc tattttcttt taggtgagga tatcccgcat gaaacccatt
     3901 ataaaaacct gaaacaggca gaggaatggg gtttcaggat ttctgaggga ataagcatct
     3961 gccggaatgc tgctgaaata atggattttg ttaaaaaatg ggatgaagac agaaaaaaac
     4021 tgatatatga tattgatggc gtggtgatca aggtcaactc ccttgccctt cagcaggaag
     4081 ccggattcac cgccaaatcc cccaggtggg cagccgcttt caagttcagg gcagaacagg
     4141 ttaccaccag actgctctca gtatctttcc aggtgggaag gacaggaaca gtaacccctg
     4201 tggccaacct tgatcccgta tttcttgccg gatccacagt acgaagagca tctctgcaca
     4261 atgccgacca gatcggaata cttgacctgc atataaatga tatggtttac gttgagaaag
     4321 gcggggaaat aataccgaaa ataacgggtg tggacctgtc ggagagagat gaaaacaata
     4381 taaaagtcag cttcatcacc aggtgtccgg aatgcggaac tcccctggaa aggaacgagg
     4441 gagaagcaaa tcatttctgt ccgaactatc ttcactgccc tccccagatc accggaagga
     4501 tagagcattt tatcagccgc agggcaatgg ccatcgaggg actcggcacg gaaaccgttg
     4561 acctgctgtt caaagaagga ctgataagaa attttgcaga tctgtatgaa ctaagaactg
     4621 aacagcttgt accccttgag cgcctcgggg aaaaatcggc ccggaacatt atcaacagta
     4681 tcagggagtc tgcagggatt ccatatagcc gtgtgatttt tgcactgggt atcaggcatg
     4741 tcggagaaac aactgcaagg actattgcca gaaatttcag atctgttgat gagctgatga
     4801 atgctgattt tgaaaggctg acaggtatca gggaaatcgg tccgaggatt gcctccagca
     4861 tcatttcata tttcagggat gaagagaata ttgagataat ccggagactg aaatcttacg
     4921 gactgaatct cagggaagaa aggaaggaag aaaggaagga agaagatcag gaagaacacg
     4981 aaactgagga agggagaaag gaaggaatga aggaaggaaa aaaagaggcg gtccttgccg
     5041 ggaaatcaat tgttatttca ggagtattcc accggcacag ccgtgatgaa tacagggaga
     5101 taatcgaaaa aaggggaggg aaaaatgttt cttccccgtc gtcgaacact tcattcatcc
     5161 tggccggaga aaacatgggg ccctcaaaaa gagaaaaggc tgaatccctt ggaattcccc
     5221 tgatcagtga ggaggaattt ctggaattaa tcggtgaaga ataaaaaaat ctcctgcagg
     5281 ataaaaaaaa acacttaggt ttgcaatatg gtactattgg gagaattcag acagaaggtc
     5341 ggggtataca tcctggacag aaaagggtca aggtccaagc gtaaggtcca ttactcgaat
     5401 atagaaagcg taaaaaagat agggatagta tgggatgcgt caaacaatga agaattagcc
     5461 gttttgtcgc aattccaccg ccagctgaat gaaaaggaca ta
//
LOCUS       DAOSPX010000218         5448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13674_length_5448_cov_4.377897, whole genome shotgun sequence.
ACCESSION   DAOSPX010000218 DAOSPX010000000
VERSION     DAOSPX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13674_length_5448_cov_4.377897"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLZ75_10165"
     CDS             complement(<1..264)
                     /locus_tag="PLZ75_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72795.1"
                     /translation="MPGFFERFRPHASRKALSLIAGTMWAGVGIMLTLRAVGWLRDYQ
                     GNVWLFAIPGLAASLLIHHFGFMKMADRNLDRISHLPDRPCVFS"
     gene            556..2196
                     /locus_tag="PLZ75_10170"
     CDS             556..2196
                     /locus_tag="PLZ75_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HOS72796.1"
                     /translation="MRKLFTIIAAVCTAGSVFAGGLVTNTNQSSLYTRLQSRNASTLI
                     DAVYYNPAGVTRLGSGIFLSLNNQSVWQTTTVLNDYTYLAGKPREYTGKVKAPLFPGI
                     YAAANLGKLSVSAGVNPIGGGGGAKYETGLPSFEMPISDLVPLLASQKIPATQYAADI
                     YFEGTSVYMGYQVNVAYKLNDIISAGVGLRVVSAKNTYKGNLLNISINPNFEKFGEEF
                     KGSMVKARTFFTAGETFFNSNSALFTGTASKLQPIITGGGGSLPLAQGANVGMKPEDI
                     QGIQALLAAIGLPADEIAAIDIQSAQGYLNTAGEDFLEKAGAMREYAGATADRYVDAA
                     QTGTGYSPILSLNISPVSNLNIGIKYEFKTKLNLLTKVYDGKGGGVFTDRDTTIADMP
                     AMLAAGFEFRPIETFMVTGSANIYFDKKVDYDGSKTKDIKMIDKNFTEYALGVELGLT
                     PWLKVSGGWLGTFTGVNSDYQNDQRFSLNTNSVGAGLGIKLAPMVSLNVGGQYTTYKE
                     GSKNFNHMLGQIPVPVTEKYNKNTMVIGAGLDFHFGLK"
     gene            2445..3479
                     /locus_tag="PLZ75_10175"
     CDS             2445..3479
                     /locus_tag="PLZ75_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS72797.1"
                     /translation="MVRYNTLFTLVFLLFSAGCRNAGNYSASEEPAVNIITERAGFSE
                     TAEEIKLSGNIEGFKTVRLGFMVAGKVSYISANEGEYIRKGSLISYLDPASYEIAKEL
                     ADIQFNQVQDEYNRLKLMYDNNSLSESDFQKITFGLQQARAQQKLHTRNLEETRLYSP
                     IEGVLLKKLCETGEIIGTGMPLFVVSDIRKVKISAFIPGEELHKAGTGQKAVIEVASV
                     NKTFEGVIAEVGSAADPSSRSFSLKIEAENPGMLMRPGMTASVRIESPEAGRAITLPP
                     QAVLHDASKQSYVYFVDTLSGKAFRRNVGTGRIIRDKIEITYGLSGNEIIVTGGQHRL
                     TEGSSVTISK"
     gene            3487..>5448
                     /locus_tag="PLZ75_10180"
     CDS             3487..>5448
                     /locus_tag="PLZ75_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOS72798.1"
                     /translation="MNFVKASIKHRQVTFTILFLLFAFGLNSLINMPRREDPRMTIPA
                     GLVIAYLPGANVYQVEDQLTSRLEDFIFRFEEVRKEKTYSVTSDGMVTIYVWLHDNVK
                     KPEMFWNKLRHQWNVVKVTDLPEGVIGPVVNSDFGDTESMIIALESDDEDFTGMKHYM
                     NGLADRLRQIPAISKLELTGTRGEQITVTLSSEKLTQYGISLQQVVKLIQSRNTISSS
                     GSIRTGILATPLHTSGYYISEADIREQIAGASETGALIRLSDISDIRRDYAEAESSVR
                     VNGKNALLLAVQMHEGKNIVKTGKEVEKIVAEFSEQLPSGKSLTTIVNQPMIVGENIL
                     KFMKEFLMAITAVILIVFLLLPFRIALVSASAIPMTVSITFALMHVLGIELHQVSLAS
                     LIAVLGLVVDDAIIIADNYVELLDRGHDRWTAAWRSASDLVVPVFTATATIIAAFLPL
                     IILKGVIGEFIRALPLTVTISLSASFLVAMLMTPVTCFLFIGRGLHGKQDAAGKSRRT
                     FLDLLQNSYNRAIDWCMRNRTVTIGGSLITVALAALLFITAVRHKFFPEAERNQFIIE
                     LWMPAGTKLEKTEEAIDRMGKLVEGDERVRSFAVFSGRSAPRFYYNYSPEIPSPEYAQ
                     ILINTVNKKATMSLYNELREKAGSIIPEGR"
BASE COUNT         1612 a         1270 c         1303 g         1263 t
ORIGIN      
        1 ggaaaagaca caaggcctgt ccggcagatg cgagatacgg tcaagattcc tgtccgccat
       61 tttcatgaag ccgaaatgat gaataagcag tgaggctgca agtcccggta ttgcaaaaag
      121 ccaaacattc ccctggtaat ccctgagcca tccgactgcc ctcagtgtaa gcattattcc
      181 tacaccggcc cacatcgtac cggctatcag cgataaagct ttcctggatg catgtggcct
      241 gaacctctca aaaaatcccg gcatggcaaa gactgaatga tgaagcagac aattttacaa
      301 attgtttgaa tcaaaacaaa ctcaaaaaca atcccggcat ggacaaagtg tggttttcga
      361 acctgattca aaaaactgta caaataatta gatatcagcc ggctatttta gaataatttg
      421 tttgaatcaa attatttcat acttttggcc cgtaatactg aatgaaatgc agcttaaacc
      481 cgcattccag actgaacata aacaggaatc ccctgtttag aggattaatt aaaattaata
      541 tttaaactcg ttcccatgag aaaattattt acaatcattg cagcggtatg tacagccgga
      601 tcagttttcg ccggaggact ggttacaaac accaatcaaa gcagtcttta cacccgctta
      661 caaagccgta acgcatccac actcatcgat gccgtttatt acaatcccgc cggagtgacc
      721 aggcttggca gcggaatttt tctttccctg aacaatcagt cggtatggca gacaacaacc
      781 gtactgaatg attacacata cctggccggc aagcccaggg aatataccgg caaggtaaag
      841 gctcccctgt ttcccggaat ttacgcggcc gccaacctgg gtaaactttc cgtatctgca
      901 ggtgtcaacc cgatcggtgg cgggggcggt gctaaatatg aaaccggact gccgtccttt
      961 gaaatgccca tctccgacct ggttcccctg cttgcttccc agaaaatacc cgcaacacaa
     1021 tacgctgccg acatatattt tgaaggaaca tcggtttaca tgggctacca ggtgaacgta
     1081 gcctataaac tgaatgacat tatttcagca ggtgtcggcc tgagggtggt cagcgcaaaa
     1141 aacacctaca agggcaatct gcttaatatc agcataaacc ccaacttcga gaaattcggg
     1201 gaggaattta agggatcaat ggtaaaggcc agaacctttt tcactgccgg tgaaacattt
     1261 tttaattcta attcggcact gttcacagga actgcttcaa aattgcagcc aattattact
     1321 gggggaggcg ggtcattacc tctggcacag ggcgccaatg taggaatgaa accagaggat
     1381 atccagggga tccaggccct tctggctgcc ataggtctgc ctgctgacga gatagccgct
     1441 atagacattc agtctgcaca gggatatctc aatactgccg gagaagattt tcttgaaaag
     1501 gctggtgcca tgagagaata tgccggcgca acggcagacc gttacgttga tgcagcacag
     1561 acaggaaccg gttattcacc catcctcagt ttaaacattt ctccggtcag caatctcaac
     1621 atcggaatta aatacgaatt caaaacaaaa ctcaacctgc tgacaaaagt atatgacgga
     1681 aagggcggag gtgtttttac cgacagggat acaacaatag cggatatgcc ggcaatgctt
     1741 gctgcagggt ttgagttccg gccaatcgaa acattcatgg ttacaggaag tgcaaatatc
     1801 tatttcgaca agaaagtgga ttatgacgga agcaagacga aggatataaa aatgatcgac
     1861 aaaaatttca ccgaatatgc actgggagtt gaactcggtc ttactccctg gctgaaagta
     1921 agcggaggat ggctcggcac tttcaccggg gttaattcag actatcagaa cgatcagaga
     1981 ttcagcctca acacaaattc agtgggagcc ggtctgggaa taaagctggc cccgatggta
     2041 agcctcaatg tgggaggtca gtataccaca tacaaggaag gatcaaaaaa tttcaatcat
     2101 atgcttggcc agatacctgt acccgtgacg gaaaaatata acaaaaacac aatggtcata
     2161 ggcgcaggac tcgatttcca cttcggcctg aaataaaaaa ctccataaca ggaaaattaa
     2221 aaagactgat ctttttggat cagtcttttt tttttgcaac atttaaagaa ttaatcctga
     2281 ccggaataat attctcctga attcagaagt cccggcctcc ggcttccgca ggaacagtgt
     2341 tgtgattcag aacgatacaa gcttgcttat catgaatcag gaccagatta aaccggccgg
     2401 acagaatgga ctaaaacaag aaaatgttcc aaaaatttcc tgaaatggta agatacaata
     2461 ctctttttac cctggtattc ctcctgtttt ccgcgggatg caggaatgcc ggcaattatt
     2521 cagcctctga agagccggca gtaaacatca ttacagaaag ggccgggttc tcggaaactg
     2581 ctgaagaaat aaaactaagc ggcaatatcg agggctttaa aaccgtcagg ctgggtttca
     2641 tggtagccgg gaaagtcagc tatatttccg caaacgaggg tgaatacata aggaagggaa
     2701 gcctgatctc gtacctcgat ccggcaagct atgaaattgc aaaggaactt gcagatatcc
     2761 agtttaacca ggtacaggat gaatacaaca ggctcaaact gatgtatgac aataacagtc
     2821 tgagtgagag tgattttcaa aagataacat tcggactgca gcaggcccgg gcccagcaga
     2881 aacttcatac ccggaacctg gaggaaacca ggctttattc tccgatagaa ggagtactcc
     2941 tgaaaaagct ttgtgaaacc ggggaaataa tcggcacagg aatgcccctc ttcgttgtat
     3001 cagatatacg gaaagtaaag atcagcgcct ttattccggg tgaggaactg cataaggcag
     3061 gaacagggca gaaagctgtg attgaggtgg cctccgtgaa taaaacattt gaaggtgtga
     3121 tagccgaggt ggggtctgca gccgatccct cttcaaggtc tttttcactg aaaattgaag
     3181 cagagaatcc cgggatgctg atgcgcccgg gaatgacagc ctcagtaagg atcgaatctc
     3241 cggaggccgg cagagccatc acccttccgc cgcaggctgt attgcacgac gccagcaaac
     3301 agagctatgt ctattttgtg gatacccttt ccggaaaggc tttcaggagg aatgtcggca
     3361 caggacgtat catccgtgat aaaatagaaa taacatacgg cctgtcggga aatgaaataa
     3421 tcgtaaccgg cggccagcac aggctgacag aaggaagctc cgttaccata agtaaataac
     3481 cccgtcatga attttgtaaa agcttccatt aaacaccggc aggtaacatt cactatatta
     3541 ttcctgcttt ttgccttcgg attaaattca ctgatcaaca tgccccggcg tgaagatccc
     3601 aggatgacca ttcctgccgg acttgtgatt gcctatctcc cgggagccaa tgtttaccag
     3661 gttgaagacc agcttacatc caggctcgag gattttatat tccgctttga ggaagtccgg
     3721 aaggaaaaaa catattctgt aacatccgac ggaatggtaa cgatctacgt ctggctccac
     3781 gacaacgtaa agaaacctga aatgttctgg aacaagctga ggcatcagtg gaacgttgtg
     3841 aaagtgaccg acctcccgga aggagtaata ggtcctgtgg taaattcaga tttcggcgat
     3901 accgaatcca tgattatagc actggaaagt gatgatgaag actttaccgg catgaagcat
     3961 tatatgaacg gccttgcgga ccggctcaga caaatccctg caatatcgaa actggaactg
     4021 acaggaaccc ggggggaaca gattacagtc actttaagtt cagaaaagct gacacagtac
     4081 ggaatcagcc tgcagcaggt ggtgaaactt attcaatccc gcaatacaat cagttcttca
     4141 ggcagtatca ggaccgggat tctcgccaca ccgctccata catccggcta ttacatctcg
     4201 gaagccgaca taagggagca gatcgcggga gcatcggaaa caggtgcgct gatcaggctg
     4261 tcagatattt ctgatatccg gagggattat gcggaggcgg aaagctccgt tagggtaaac
     4321 gggaaaaatg ctctcctgct tgccgtgcag atgcatgaag gaaagaatat tgtaaaaacc
     4381 gggaaagagg tggaaaagat tgtggcagag ttctcggaac agctgccgtc ggggaaaagc
     4441 cttaccacca ttgtgaatca gccgatgata gtcggagaga atatcctgaa gttcatgaag
     4501 gaattcctca tggcaattac tgccgtcata ctgatagttt tccttctcct gcccttcagg
     4561 attgcacttg tgtcggcatc tgcaattccg atgacggttt ccataacctt cgccctgatg
     4621 catgtgctgg gaatcgaact gcaccaggta tccctggctt ccctgatagc tgtactgggc
     4681 ctcgttgttg acgatgcgat aattattgcc gataattatg ttgaacttct tgacagggga
     4741 catgaccggt ggacagcagc atggagaagt gccagcgacc ttgttgttcc cgtgtttact
     4801 gcaacagcca ccatcatagc agcattcctc ccgcttatca tcctgaaagg cgtgataggg
     4861 gaattcatcc gtgcactgcc gcttacagta accatctccc tgtcggcttc cttcctcgtg
     4921 gcaatgctga tgactcccgt gacgtgcttt cttttcatag gccgcggact gcatggcaaa
     4981 caggatgcgg caggaaaaag cagaaggacc ttcctggatc tccttcagaa ctcatacaac
     5041 agggccatag actggtgcat gaggaaccgt acagtcacaa tcgggggcag cctgatcacc
     5101 gttgcactgg ctgccctgct tttcataact gctgtcaggc ataaattctt cccggaagcc
     5161 gaaagaaacc agtttatcat cgaactatgg atgcccgccg gcacaaagct ggaaaaaacg
     5221 gaagaagcaa tagacaggat ggggaaactt gttgaagggg atgagagagt caggtcgttt
     5281 gccgtattct ctggcagaag cgctcccagg ttctactata actattcgcc tgaaatacct
     5341 tccccggaat atgcacagat actgataaac acggtgaaca aaaaggccac catgtcactg
     5401 tacaacgagc tgagggagaa agccggatcc atcatacccg aaggaagg
//
LOCUS       DAOSPX010000219         5438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13721_length_5438_cov_3.724317, whole genome shotgun sequence.
ACCESSION   DAOSPX010000219 DAOSPX010000000
VERSION     DAOSPX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13721_length_5438_cov_3.724317"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLZ75_10185"
     CDS             complement(<1..764)
                     /locus_tag="PLZ75_10185"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOS72799.1"
                     /translation="MENEILVVQNGEQDDHAFKWEFPGGKRKKGESGEECIIREIREE
                     LFMDIVICGSLDPVVYDYGHKKIRLFPFVCDTLDELPVLTEHIDFRWCMEEELGTIDF
                     SGADLHVLKQYTGDICGSSSPAAAEPLGGIDAGTEKEIKGMINSMISTREADWLAAGA
                     PDNPELILKLLEYSYSDDRKLSFRASWTLTKISDKNPEIIIPHIPGMIERLDETVNES
                     TRRSFLRIISLRNITLLSLKHHGILADHCFRALNSKL"
     gene            complement(794..1258)
                     /locus_tag="PLZ75_10190"
     CDS             complement(794..1258)
                     /locus_tag="PLZ75_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOS72800.1"
                     /translation="MKELNLPEYSFRIIERDGKRMILDVLRRKYVRLTPEEWVRQNFV
                     QYLIQEGHYPSGLIGIEVPAPFNRLKKRVDVLVHDRSGRPLMIVECKSYDLSLDDAVF
                     EQIATYNMKYNVPFLVVTNGMHHYACRLGAGSARPVYLNVIPLYEELTDLIQ"
     gene            1317..2090
                     /locus_tag="PLZ75_10195"
     CDS             1317..2090
                     /locus_tag="PLZ75_10195"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOS72801.1"
                     /translation="MKTKEDIVNNWLPRYTGKSIESFGKYFLLTNFISYVELFAKLNN
                     VKTEGLDKNMPSATSEDGITIINFGMGSPNAATVMDLLSAVSPSAVLFLGKCGGLKKR
                     NNLGDLILPIAAIRSDGTSNDYLPVEVPALPAFQLQSAISASIIRHNKEYWTGTVYTT
                     NRRVWEYDARFKKYLVKTRAMAIDMETATIFTVGFANEISTGALLLVSDQPMISAGIK
                     TEESDRKVSDKFTEIHLKIGIDALREIKNNGISVKHLRF"
     gene            2092..3102
                     /gene="holA"
                     /locus_tag="PLZ75_10200"
     CDS             2092..3102
                     /gene="holA"
                     /locus_tag="PLZ75_10200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOS72802.1"
                     /translation="MVTSYEEIISGLRKRIFKPVYFLAGEEPYYIDLITDHIEQNVLP
                     EADRPFNQMVLYGEDTSVNDIIEVSRRYPMMATHQVVIVKEAQSVRNIENLSIYIERP
                     LPSTILVINYKYKVLDRRTRLYRLLGNHGIYFVSNKLRDYQIPGWIERFLMEEGIKSE
                     PDVSVMLGEYLGNDLHKIANELNKLIITLSPGNSVISAGLVEKNIGISKDYNNFELQK
                     AVGERNILKANMIVSHFASNPRDNPLTLTIASLFSYFNKLLAYHYLTDKSKNSVAAAL
                     NIHPFFVRDYETAAMTYSVTKIIQIISLLRIFDMKSKGVGDLGSEEGDLLRELVYKIL
                     HL"
     gene            complement(3192..3770)
                     /locus_tag="PLZ75_10205"
     CDS             complement(3192..3770)
                     /locus_tag="PLZ75_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOS72803.1"
                     /translation="MDLFTIMPEDKIPGKGKILISEPFLPDTFFNRTIVYLTEHNSEG
                     SVGFILNKKLDIKVSDAISGFEGWEGYLNMGGPVAPDTLHYLHGLGDRITRSVKIDEG
                     IWWGGDIDILREMAINHSLPTSQIRFFLGYSGWSAGQLERELRENSWVIARVPADVVL
                     DDRITMSWKRVLRGLKNKYRIWADFPESPDMN"
     gene            complement(3930..>5438)
                     /locus_tag="PLZ75_10210"
     CDS             complement(3930..>5438)
                     /locus_tag="PLZ75_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOS72804.1"
                     /translation="SPERLKWEDDFDPIRKMREWIIEQKIAGPEELDSIAAEAENEAR
                     ESRKKGWEMFQNPIRVERDALVRIINERTCRCSEEAKEASVEVVTAELAKILHPIRKD
                     NFMAARKILRNVCETCYLSDNLNEELHAWLKRNYQDAELSYDTFLYNDMPTSVLKVKP
                     IPPVFSENSPEVPGRQILRDNYDRLFAKYPLLVTFGEDTGYIGGVNQSLEGLQKKYGE
                     LRITDTGIREATILGQGIGLALRGMRPIAEIQYLDYLMYALQILSDDLATTHYRTAGR
                     MVAPLIISTRGHRLEGIWHSGSPMSMLINSVRGVYVCSPRDMTRAAGFYNTLLQGDDP
                     AIVIEPLNGYRLRERMPDNLGEFRIPLGVPEILSEGKDVTLVTYGSTVRIAAEAVRQL
                     ALHDISVELIDAQTLVPFDLNAVILESLKKTNRLLLLDEDVPGGTTAYMMQEILEKQG
                     GWNYLDSAPRTLTGKEHRPAYTTDGDYFSKPSAEDVFDAVYGMMKEAVPSKF"
BASE COUNT         1458 a         1356 c         1172 g         1452 t
ORIGIN      
        1 agtttggaat tcagggctct gaaacaatgg tcagccagga tcccgtgatg tttaaggctc
       61 aatagcgtga tatttctcag ggaaatgatc ctcaggaagg accggcgggt gctttcatta
      121 acggtctcat caagcctttc aatcattccc ggtatgtgcg gaattatgat ttcaggattt
      181 ttatcggata tttttgtcag ggtccatgaa gcacggaacg acaatttcct gtcatcgctg
      241 tatgaatatt ccagtaattt cagaatgagt tccgggttat ccggtgcacc ggcagccagc
      301 cagtccgctt ccctggtact gatcatcgaa ttgatcattc cctttatctc tttttccgta
      361 cccgcatcga tacccccgag gggttcagcg gctgccgggg aagatgatcc gcatatatcg
      421 cccgtgtact gtttcagaac atgcaggtcg gctccggaaa agtcaatcgt ccccagttct
      481 tcctccatgc accacctgaa atcaatatgt tccgtaagaa ccggcagttc atcaagggta
      541 tcacagacaa agggaaaaag ccttattttc ttgtgtccgt aatcataaac aacaggatca
      601 aggctgccgc agataactat atccatgaac agttcctccc ttatttccct tattatgcat
      661 tcctccccgc tttcaccctt ctttctcttt cccccaggga attcccactt gaaggcatga
      721 tcgtcctgtt ccccgttctg taccacaagg atttcatttt ccacattcct gataattgca
      781 caacagacat caatcattga atcagatctg taagttcttc ataaaggggt atgacgttca
      841 gatatacagg tctggcagat ccggctccca gcctgcatgc atagtggtgc ataccgttcg
      901 ttaccacaag gaagggaaca ttgtacttca tattgtaggt tgcaatctgc tcaaaaacag
      961 catcatccag ggaaaggtca tatgatttgc attcaactat cataagcggt ctgccgctgc
     1021 ggtcatgaac aagaacatcc actctctttt tcagcctgtt gaagggagcc ggcacttcaa
     1081 ttccaataag tccggaagga taatgcccct cctgaatcag gtattgtaca aaattctgtc
     1141 ttacccactc ttcaggagtc agcctcacat actttctcct gaggacatcg agaatcatcc
     1201 tcttgccgtc cctttcaatg atcctgaacg aatattccgg cagatttaac tctttcaact
     1261 ttgttttgta cttttgaacg caaatatatt gatataatca atacaggtct gcaacaatga
     1321 aaaccaaaga agacattgtc aacaactggc ttccgagata taccgggaaa agcatagaat
     1381 cgttcggcaa atattttctc ctgacgaatt ttatcagtta tgttgagctt tttgcaaaac
     1441 tgaacaatgt aaaaacagag ggtcttgaca agaatatgcc cagcgccacc tccgaggatg
     1501 gaatcaccat tatcaatttc ggaatgggca gtcccaatgc cgcaacggtg atggatctcc
     1561 tcagtgcagt atcgcccagc gcggtattgt tcctgggcaa gtgcggaggc ctgaagaaaa
     1621 ggaacaacct gggtgacctt attcttccca ttgccgccat caggagtgac ggaacctcca
     1681 atgactacct gcccgtggag gtgcctgcac tgccggcttt ccagctgcag agcgccatat
     1741 ccgcgtcaat aatccggcat aacaaggaat actggacggg aacggtctat actacaaacc
     1801 gcagggtgtg ggaatatgat gccaggttta aaaaatatct ggtaaaaaca agggccatgg
     1861 ccattgatat ggaaactgct actatattta cggtgggatt cgcgaatgaa atttccacgg
     1921 gagcattgct tcttgtttca gatcagccca tgatatcagc aggcatcaag acggaagaaa
     1981 gtgacaggaa ggtaagcgat aaatttacag aaatacatct gaagatcgga atagatgccc
     2041 tgcgtgaaat aaagaacaac ggaatctcgg taaaacatct caggttctga aatggtaaca
     2101 agctacgaag aaattatttc cggactgagg aaaagaatct tcaaacctgt ttatttcctc
     2161 gcaggagaag agccgtatta tattgacctc ataaccgacc atattgagca gaacgtgctg
     2221 cccgaggcag acaggccgtt caaccagatg gtattgtacg gagaggatac ttctgtcaac
     2281 gacattattg aagtatccag gcgctatccc atgatggcca cacatcaggt cgtgattgtc
     2341 aaggaggcac aatcagtaag aaatatcgag aatctgtcaa tctatattga aaggcccctg
     2401 ccatcaacca ttctggtaat caattataaa tacaaggttc tggatagaag gaccaggctg
     2461 tacagactcc ttggaaacca cggtatttat ttcgtctcca acaagctgag ggattaccag
     2521 atccctggct ggatcgaaag gtttctgatg gaggaaggta taaaaagcga gcccgacgtc
     2581 agcgtaatgc tgggtgagta tcttggcaat gaccttcata agattgccaa tgagctgaat
     2641 aagctgataa tcacgctgtc ccccggcaat tccgtaataa gtgccggact ggttgaaaaa
     2701 aacatcggaa tcagcaagga ttacaacaat ttcgaactgc aaaaagctgt cggcgaaagg
     2761 aacatactaa aggccaatat gatagtcagt cacttcgcct ccaatccccg ggataatccc
     2821 ctgactctca caatagcctc cctcttcagc tacttcaaca aactgctggc ataccattat
     2881 ctcactgaca agtccaaaaa cagcgtggcg gcggcactta atatccatcc cttctttgta
     2941 agggactatg aaactgctgc catgacctac agtgttacaa agataattca gataatcagt
     3001 ctgctgagaa tttttgacat gaaatcaaaa ggagtcgggg atctgggaag cgaagaaggc
     3061 gacctgctca gggaactggt atataaaata ttacacctgt agaaaaagtg gcagttgata
     3121 ctgcctgact aacgtaatac ctgactaagc tgtctggtat tagatatccg gttttaaata
     3181 tacggaggac actaattcat atcaggtgat tcgggaaaat cggcccagat ccggtattta
     3241 ttctttaatc ccctgagaac ccgtttccag ctcatggtaa tccggtcgtc caggacaaca
     3301 tctgcaggaa ctcttgcaat aacccacgag ttttccctga gctcacgctc cagctgtccg
     3361 gctgaccagc ccgaatatcc cagaaagaac ctgatctgcg aagtgggaag actgtggttt
     3421 atcgccatct ccctgaggat atcaatatcc ccgccccacc agataccttc atcaatcttc
     3481 acgctccgtg ttatccggtc gcccagtccg tgcaggtagt gaagggtgtc aggtgcaaca
     3541 ggacctccca tgttcagata tccctcccag ccctcaaagc ctgaaattgc atcacttacc
     3601 tttatatcaa gcttcttgtt cagtataaat cccacggaac cttcggaatt atgctcggta
     3661 agatatacta tggtcctgtt gaaaaaggta tcgggaagga aaggttctga aataagaatc
     3721 ttgccctttc ccggtatctt gtcttcaggc atgattgtaa acaaatccat ctcctctttc
     3781 atatcgcaat attgacagga caatttacat atttttatta tactgaccat ggatatgcgc
     3841 ctttaagaat gcaaaatcct cctggtccgt caatttcatc agaatcacag gatataaaat
     3901 aattctgccg gagttttctc tttctgtcat cagaattttg acgggactgc ttctttcatc
     3961 attccataga cggcatcaaa aacatcctct gcactgggct ttgaaaaata atccccgtcg
     4021 gtggtatatg ccggacggtg ttcctttccc gtaagggtcc tgggagctga atcaagataa
     4081 ttccatccgc cctgcttctc caggatctcc tgcatcatat acgctgttgt gccaccggga
     4141 acgtcctcgt ccagtaaaag cagcctgttt gtttttttca gggattcaag tattacggca
     4201 ttcagatcaa agggaacaag tgtctgtgca tcaataagct caaccgaaat gtcgtgcagt
     4261 gccagctgcc tgacagcctc tgcagctatt ctgaccgtgg atccgtatgt cacaagggtc
     4321 acgtccttac cctcgctcag gatttcgggt acacccaggg gtattctgaa ttctcccagg
     4381 ttatcgggca tcctctccct tagcctgtat ccgttcaggg gctcaatgac tatggcagga
     4441 tcatcaccct gcagaagtgt attatagaaa ccggcagccc ttgtcatatc cctcggactg
     4501 cagacatata cccctctcac ggagtttata agcatgctca tgggcgaacc ggagtgccag
     4561 atgccttcca ggcggtgacc cctggtcgaa attatcagag gagcaaccat tctgccggca
     4621 gtacggtaat gggttgttgc cagatcatcg ctgagtatct gaagagcata catcaggtag
     4681 tccaggtact ggatttcggc aatcggccgc atgcccctga gagccagccc tataccctgg
     4741 ccaagaattg tggcctccct aatccccgta tccgtgatac ggagttcgcc gtattttttc
     4801 tgcagacctt caagcgactg gttgaccccc cctatataac ccgtatcttc tccgaatgtt
     4861 accagcagcg ggtatttcgc aaacagcctg tcatagttat ccctcagtat ctggcggccg
     4921 ggaacttccg gcgagttttc ggagaaaaca ggaggtatgg gctttacctt caggactgag
     4981 gtcggcatat cattgtaaag gaaggtgtca tagcttaatt cagcgtcctg ataatttctt
     5041 ttaagccatg catgaagttc ctcattcaga ttatcggaaa gatagcatgt ttcacaaacg
     5101 ttcctgagta tcttcctggc tgccatgaaa ttatctttac ggataggatg aaggatcttt
     5161 gccagctcag ccgttaccac ttccaccgaa gcctcctttg cctcctcgct gcatctgcat
     5221 gtacgctcat ttattatcct taccagggca tcacgctcca ccctgatcgg attctggaac
     5281 atttcccatc cctttttcct tgattccctg gcttcatttt ccgcttctgc tgcaattgaa
     5341 tcgagttcct caggacctgc aatcttctgt tcaattatcc attcccgcat tttgcgtatg
     5401 ggatcgaaat catcttccca tttaagcctt tcaggact
//
LOCUS       DAOSPX010000220         5390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13864_length_5390_cov_4.203936, whole genome shotgun sequence.
ACCESSION   DAOSPX010000220 DAOSPX010000000
VERSION     DAOSPX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13864_length_5390_cov_4.203936"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1284
                     /locus_tag="PLZ75_10215"
     CDS             271..1284
                     /locus_tag="PLZ75_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOS72805.1"
                     /translation="MSETRFYHFTGKGMLHRLKTHEEAIMALGGGGFIWIDFFKPEIG
                     ELHSLVDTLGVHPLSVEDCLDSSQMPKIEHFPGNTFMIFKDFSYKDKMLYIDEVDFFI
                     GKNFLVTVSGTGSDDRRPLNNIINIIEKDPGIARSEPAFLLHVILDYVVDQKFHAFEN
                     LEDELELAEDQIIDAPDKFRVNELLRIRKDLLNLRKNLYHEREILVKICRMDCPFIAG
                     KAIFAFRDVYDHLVRFFELSENYREIVTSLMELYSSLLNNMMTKVSNETNATMRRLTF
                     ITTIFMPLTLIASIGGMSEYTMITGGDNWKTSYLLLTAGLVLIAVISYFFLRSLERRK
                     KRH"
     gene            complement(1309..1551)
                     /locus_tag="PLZ75_10220"
     CDS             complement(1309..1551)
                     /locus_tag="PLZ75_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72806.1"
                     /translation="MDKAERILVFNNEIEARLLGEILLSKGIPHIIRSFHDSAYDGLF
                     QLQSGWGVLESPENFRDEIMKIYNEMTSPENRTETV"
     gene            complement(1853..4405)
                     /locus_tag="PLZ75_10225"
     CDS             complement(1853..4405)
                     /locus_tag="PLZ75_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOS72807.1"
                     /translation="MVRGNVYGRLPCTLSGRESDSYRGSGLKGNVKTILIAAGVIIIP
                     VLLSPLPRFRSPLSTVVEAADGSLLGARIAEDGQWRFPPSGEVPDRFEKALLTFEDRY
                     FYLHPGINPVSLARALVTNIKAGKILSGGSTVTMQVARLSSGNIRRSYSAKLSEMLSA
                     LKLELFRSKKKILGIYAANAPFGGNTVGLDAAAWRYLGKAPEDMTWAEAAVLAVLPNS
                     PALVHPGRNQTILRARRDELLGKLYKRKYIDSLTLMLSLDEPLPSEPGKLPSATPHLT
                     DYFLKHGRGERIRTTIDPLLQERANAVIRAHQKELSGNLIHNSACIIVKVETGEVLAY
                     AGNSTPESAVRHGNDVDIIRSPRSTGSILKPLLYAAMQQSGDILPNSLVPDIPSRFQG
                     FTPKNFDLSYSGAVPASLALSQSLNIPSVRMLQQYSPERMLALLRKTGFTTFTRPASH
                     YGLSLILGGGETTLWELAGVYSSLSRVLNRYNREGKYNDADYHMPVLISPDTPGTASL
                     PASVSVSQLRPAPQPARGAVSATSPQPAGKNRKSKPENFSSVPVLSASSIWLTYEALR
                     KVNRPEGESGWQFFSSSGTIAWKTGTSFGFRDAWAVGTTPGYVIAVWAGNADGEGRPG
                     LTGTAAAAPILFDLAGITGNMELFSEPKEWFTEPSDEMTMVRVCSASGYRAGPDCPET
                     MEIMACVNGLRSEACPYHRLIHLDKSGTMQVNTDCAPAASIRNEPWFILPPAMEFFYR
                     RKHPGYKTLPPFAPGCRPGRDIPAMEFIYPTPGIKIFIPRDQTGEMTRIISEVAHRNP
                     SKKIFWHLDDKFQTSTRSIHQVEILAGPGDHTMTAVDEDGNTIICPFSITGR"
     gene            complement(4324..>5390)
                     /locus_tag="PLZ75_10230"
     CDS             complement(4324..>5390)
                     /locus_tag="PLZ75_10230"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72808.1"
                     /translation="EWRYDPRNIQTSNDQAYRLFTLALAGTPEKGAMNRLRETQDVPA
                     LSRWLLAAAFAASGRNEVAGSLLDVRNTSTETEYQPFYYGSALRDKAIILYTLTLLDK
                     KEQALPLLREVCGMLTENPWHSTQSLAWGLFAYMKWVQAMPADDKSPARVSMSFNNRQ
                     SEKSIVPGEIVSESLDMLAGSNSLTLVNNSGSPVYATLMRKGIPPASDVTAVEKGLRL
                     KVDYVNTRLEPVDHRKLATGTSFLMVVRVTNTGFSGVNDIALTRMVPSGWEIRNIRIH
                     EAEYGIRESASDYTDIRDDRVNNYFSLGRGETKTFVLVLNAAYKGEYFQPSIWCEAMY
                     TEDCHARYPGGRVTVTGDQD"
BASE COUNT         1262 a         1302 c         1334 g         1492 t
ORIGIN      
        1 cagtataaaa agagatttcc tttctccctg tatttccata aggtggttta tttgtcctgg
       61 tgctgccggg ttaatacacg aaggtggaaa aattattcga agaaatcaag aacaaatttg
      121 gagaatcagc cagagcggct gttaatcagt gatatagagc aacggagatt tttccgtaat
      181 tctttttccg ggaaccactc tttaaaaatg cattttgttt ttatgtaact tagtaatcta
      241 atttagtcag caacaaagct atcatccgtt atgtctgaga caagattcta tcattttacc
      301 ggcaagggaa tgttacacag gcttaagact catgaagaag ccattatggc tcttggtgga
      361 ggagggttta tctggatcga ttttttcaag ccggagattg gggagcttca ttctcttgta
      421 gacacccttg gggttcatcc cctgtcagtt gaggattgtc ttgacagcag tcagatgccc
      481 aagattgaac atttcccggg taatacattc atgatcttca aggatttcag ctataaagac
      541 aagatgcttt atattgatga agtggatttt ttcatcggaa agaattttct ggttacggtg
      601 agcggcactg gcagtgacga ccgcaggcct ctgaataata tcatcaatat cattgagaaa
      661 gatccgggaa tcgccagatc tgagcccgca ttcctgctgc atgtgattct cgattacgta
      721 gttgaccaga aattccatgc atttgaaaat cttgaagacg agcttgagct ggcggaagac
      781 cagatcattg atgctcctga taaattccgg gtgaacgagc tcctcagaat aagaaaggat
      841 ctgctgaatc tccggaagaa tctttaccat gaaagggaga tcctggttaa aatctgcagg
      901 atggattgtc ctttcattgc cgggaaggca atatttgcat tccgcgatgt ttatgatcac
      961 ctggtaagat tttttgaact ttcggaaaac taccgtgaaa tagtcacaag tctcatggaa
     1021 ctttacagct cccttctgaa caacatgatg acaaaagtgt ccaatgagac gaatgccacc
     1081 atgcggagac taacatttat cacaaccata ttcatgcccc tcacccttat cgccagtatt
     1141 ggcggcatgt cggaatatac catgataacg ggaggagata actggaagac ttcctatctt
     1201 ctgctgacgg cagggcttgt gctcattgca gtgatcagtt acttttttct gagaagcctt
     1261 gaaagaagaa aaaagcggca ctgacagccc ggacaataaa aaaaaatttt aaactgtttc
     1321 ggtacggttt tccggtgaag tcatttcatt atagatcttc atgatttcat ccctgaaatt
     1381 ttccggactt tccaaaacac cccaccccga ctgtaactga aacagaccgt cataggctga
     1441 atcatgaaag gatctgatga tatgcggaat cccctttgac agaaggattt ctcccagaag
     1501 ccttgcttct atttcattgt tgaaaacaag aatacgttct gctttatcca ttattctgat
     1561 tttttaatta tcatttgagc attcggtaaa tagctttgat accggtcagg ctgttagggc
     1621 atcatcatca ggtacggagc aattgcggca tcaggcccgg acgatttcag cataatttca
     1681 gaaccataca gccatacaaa atatctggat ataaagatat atttttccgt tgtttatttt
     1741 tgttttccct gattttattt tctgccaggt ctatgccggg tttcctcccg ggaatctttt
     1801 atcggacgtt tgttagatat taagccgggt tgtttccagg gaatctttat cttcatcttc
     1861 cggtgatgct gaacgggcag attatggtgt tgccgtcttc atccacggcg gtcatggtat
     1921 ggtcacccgg tccggcaagg atctcaacct ggtgaatgga tcttgttgag gtctggaatt
     1981 tgtcatcgag atgccagaat atctttttag aaggattcct gtgcgctact tctgaaatta
     2041 tcctggtcat ttcaccggtc tggtcccttg ggatgaatat ttttattccc ggtgtaggat
     2101 atatgaattc cattgcggga atgtccctgc cgggccggca gcccggggcg aagggcggaa
     2161 gcgttttata tccgggatgc ttccgccggt agaaaaattc catcgcagga ggaaggatga
     2221 accagggctc attcctgata ctggcggcag gagcacaatc tgtatttacc tgcattgtcc
     2281 cgcttttgtc aagatggatc agcctgtggt acggacaggc ttcactgcgc agaccattca
     2341 cgcaggccat tatttccatg gtttcggggc agtcgggtcc ggccctgtat ccgcttgcgg
     2401 agcagactct gaccatggtc atctcatccg acggttccgt aaaccattcc tttggttctg
     2461 aaaacaattc catgttcccc gttattccgg cgagatcaaa taatatggga gcggcggcag
     2521 cagtgcccgt cagtcccggt cttccctcac cgtccgcatt gccggcccaa acagctatta
     2581 catatccggg agttgtacct acggcccagg catcacggaa accgaagctt gttccggttt
     2641 tccatgctat agtgcctgat gaggagaaaa actgccatcc ggattcccct tccggtctgt
     2701 tgaccttcct cagggcttca taggtcagcc agatggagga tgctgaaagt accggcactg
     2761 agctgaagtt ttccggtttt gatttcctgt tttttcctgc aggctgcggt gatgttgcgg
     2821 atacggcccc gcgtgccggt tgcggtgcgg gccgaagttg cgatacggat accgatgccg
     2881 gcagggatgc cgtcccgggt gtatcagggg agatcaggac cggcatgtga tagtcggcat
     2941 cattgtactt tccttccctg ttgtatctgt tcagcactct tgacagggag gaatatactc
     3001 cggcaagttc ccagagtgtt gtttcgcctc ccccgaggat gagtgacaat ccgtaatgag
     3061 aggcaggtct ggtgaatgtg gtgaatccgg ttttcctgag aagagcgagc attctttccg
     3121 gactgtactg ctgaagcatt ctgacagagg gaatgttgag ggactgcgac aaggccaggg
     3181 aggcaggaac ggctccgctg tagctcagat cgaagttttt cggggtaaag ccctggaaac
     3241 gcgagggaat atccggcaca agactgttag ggagaatatc acctgattgc tgcatggccg
     3301 cgtaaaggag gggcttcaga atgctgcccg tacttctggg cgaccttatt atatcaacat
     3361 cattcccgtg tcttacagca ctttcaggag tactgtttcc ggcataggcc agaacttccc
     3421 ccgtttcaac ctttacaatg atgcatgccg agttgtgaat gaggtttccc gacaattcct
     3481 tctgatgtgc gcggatcact gcattcgccc tttcctgcag cagcggatct attgtggtcc
     3541 ttatcctttc gcccctgccg tgtttaagga aataatccgt gagatggggt gtcgctgaag
     3601 gaagtttccc cggttcagag ggaaggggtt catcaaggga gagcatcagt gtgagtgagt
     3661 cgatatattt ccgcttgtac agtttcccaa gaagctcatc gcgtcttgcc ctgagaattg
     3721 tctggttcct cccgggatga acgagggccg gtgaattggg aagcactgca agaacagcag
     3781 cttccgccca tgtcatgtct tcaggcgctt ttcccaggta tctccaggca gcagcgtcca
     3841 ggcctaccgt gtttccgccg aagggagcat ttgcggcata aattccgagt attttctttt
     3901 ttgaacggaa aagctccagt ttgagtgcgg aaagcatctc actcagcttt gcggaatagc
     3961 ttctacggat attcccggat gagagtctgg caacctgcat tgttacagtg cttcccccgc
     4021 tcaggatctt acccgccttt atattcgtga caagggctct tgccagtgaa accggattta
     4081 tccccggatg cagatagaag tacctgtcct cgaaggtcag caatgctttt tcaaacctgt
     4141 ccggcacctc tccggatggg gggaatctcc actggccgtc ctctgctatc cgtgcaccca
     4201 gcaggctccc gtctgctgcc tccacaaccg tagacagggg ggatctgaag cggggcaggg
     4261 gcgacagcag taccggaatg atgatcaccc cggcggcaat cagtatggtt tttacatttc
     4321 ccttcaatcc tgatccccgg taactgtcac tctccctccc ggatagcgtg catggcaatc
     4381 ttccgtatac attgcctcgc accatattga cggctggaaa tattctccct tataggcagc
     4441 attcaggaca agaacaaatg tttttgtctc tccccttccg aggctgaaat aattattcac
     4501 acggtcatcc ctgatgtctg tatagtccga cgcactttcc ctgatgccgt attctgcctc
     4561 atgtatcctg atattcctga tctcccatcc ggaagggacc attcttgtaa gtgcaatgtc
     4621 attaaccccc gaaaagccgg tgtttgtcac cctgaccacc ataaggaagg aggttcccgt
     4681 tgccagtttc cggtgatcaa caggttccag ccgggtgttc acataatcaa ccttcagtct
     4741 caatcccttt tcaacagccg taacatctga tgccgggggt attccttttc tcatcagggt
     4801 ggcatagaca ggagatccgg aattattgac cagggtgagt gaattggatc ccgcaagcat
     4861 atccaggctt tccgaaacta tttccccggg gactatgctt ttttcggatt gtctgttgtt
     4921 gaagctcatg cttacccttg ccggggattt gtcatccgca ggcatggcct gcacccattt
     4981 catatatgca aacagtcccc atgcaagtga ctgggtactg tgccacggat tttccgtcag
     5041 cattccgcat acttccctca gcaggggaag agcctgttcc tttttatcaa gaagtgtgag
     5101 ggtatacagt attattgcct tgtcgcgcag ggcgctcccg tagtaaaaag gctggtattc
     5161 cgtttccgtt gaggtattgc ggacgtccag gagagaacct gccacctcgt tccgtccgct
     5221 tgccgcgaat gctgctgcaa gcagccagcg cgagagggca ggaacatcct gtgtttccct
     5281 cagcctgttc atggcgcctt tttccggggt gccggcaagt gcaagcgtga aaaggcggta
     5341 ggcctgatcg ttagaagtct ggatattccg gggatcatac ctccattccc
//
LOCUS       DAOSPX010000221         5388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13872_length_5388_cov_3.532721, whole genome shotgun sequence.
ACCESSION   DAOSPX010000221 DAOSPX010000000
VERSION     DAOSPX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13872_length_5388_cov_3.532721"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PLZ75_10235"
     CDS             <1..895
                     /locus_tag="PLZ75_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOS72809.1"
                     /translation="THKVMIDILKNDSLNMDPRIKIYADTVKAPFKGGTSPNGYYFPP
                     YKYRGVPSMGTTPIEYRYPWQGTTVSDLSEFWRVPVLAPALMRCSEVYFALAEAKLAG
                     ILPASFSGTVQDYYRRGIDASIEWYKWLYNLAAPQIPDLMLKYIHNPAYAPGAQWTQA
                     DVDKYLNHKKITDTEVDNFKSTEMYSLSGTEEEQLEKIINQKIVALYPDVFEGWCEYR
                     RTGYPRLPIGPDESLLKGVVPRRNPWPYNERNINSESYNEALARYGGKDNRLVKFWWD
                     KNPNAPHVYQWTPPSMPRAWQ"
     gene            complement(961..2397)
                     /locus_tag="PLZ75_10240"
     CDS             complement(961..2397)
                     /locus_tag="PLZ75_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72810.1"
                     /translation="MKRRKFFTITSAGIAGMALGSSCRNSAGERGSHSGKSAVKNIVP
                     DYRTMIKSDFPSSKTRPASDRVILALIGAGAWGTALILEAASSGENILIKYVCDVDDT
                     RGGRAISELEKIQGFRPLSVRDMRTVFDDPEVDGVFIETPEHWHALATIRACQAGKDV
                     YVEKTVSHSIFEGQQMIKAAMKYERIVQCGMQNRSADYALTARDYIKSGELGDIVAVH
                     VKELLDGPVPFNEKEDSAVPDTIDWDMWLGPAPKVPYNVSRNKSWIYYWDYSGGPVTS
                     GGSIHQLDMARLILGDPGFPKSACCAGGRYFFDDGREIPDYQMTTFDYGNFVLTLETG
                     ECTPYFQKEGPEVRYGSIYPKWMQNATRIDIYGTKKMMIAGRMGGGWQAFDKDEKIVA
                     QDKGIFPLKAHILNYIDCIRTRKQPNGSIVEGHNSSVLIHLANLSYRAGNKQLLFSPE
                     YEAIVNDEEARRLSEKSYRKGFEISGEV"
     gene            2899..5322
                     /locus_tag="PLZ75_10245"
     CDS             2899..5322
                     /locus_tag="PLZ75_10245"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72811.1"
                     /translation="MKNLGICSFILFLSVTVGACRERSSGKFSGKDGQQYDQWHVIGP
                     GGGGGVFKPTLSPFNENFVLTHCDMTGAYLSHDGGRTWKMKNLWNVPLDFEFDPADSN
                     TVYTATQGFLHGEDRGSGISLLLRSSDSGETWEICYPDISSAGKAEKIQSSRFRISEL
                     VEGAIDGNIQKVEVDPEDNECIYLGIAPLFEFMGGRGSQSLPSEVYVVSTSDRGRTWR
                     RLASFPGTEILALFPGADSEKLIAFSDRACVHIDKSSGSMTAFPMPSERIIIIDGGKS
                     NNGFLIYAQAPFTAGRKGGMLLSSDLGKSWRQINEGLLSENEKDPLPSFRQALAVCEN
                     RAETVYIGIDFPRKDSVGRNDPLYCIYKTEDAGKKWEPVLVSSSRRGYITGNFSGSWM
                     EESYDPGWGGSPIDLGVAPNNPDVCYAGDNGKGYRTADGGKTWVQVYSHNNPDGSYAS
                     NGLNVTTCYGVHFDPYDRDHFFISYTDIGLFHTFSGGKSWYHSLEGIPRSWQNTCYDI
                     EFDPGVRGRVWSAWANAHDLPRTKMFGGRGFGGYEGGVAVSEDGGLTWKKSSEGLPGN
                     SICTNVLLDPASPENNRTLYVSVFDRGVYKTEDDGKTWNIVNNGFGKNLFAWQLRQNS
                     RGRLFALFARGESAGKTVDGAVYYSDDRAATWTQLKLPENVNGPHDLLVDPLNPDIMY
                     VSCWPRSDLGPDAKGGVIKTTDGGKSWTRVFDERIRVNSAGMDPRNPEIIYINTFQNA
                     AYRSEDSGETWQRIEGYRFKWGQRPVPDVNNPGMLYLTTYGGSVFHGPAKGIPGIPDD
                     ITNMPEGWW"
BASE COUNT         1434 a         1193 c         1307 g         1454 t
ORIGIN      
        1 tacccataag gttatgattg acatcctgaa gaatgattcg ctaaacatgg atccgcgtat
       61 aaaaatttat gccgatactg taaaagcacc cttcaagggc ggaacatccc ccaacggata
      121 ctatttcccg ccctataaat accgcggagt accctcaatg ggaactacac ccattgagta
      181 cagatatccg tggcaaggaa ctaccgtatc cgacttgagt gaattctgga gagtacctgt
      241 tcttgcacct gcactgatga gatgctcgga agtctatttc gcacttgccg aggcaaagct
      301 tgcagggatt ttgccggcat cgttttcagg tacggttcag gattattaca gaagaggtat
      361 tgatgcttct atcgagtggt acaagtggtt atataatctt gcagccccgc agatacccga
      421 tttaatgctc aagtacatac ataatccggc ttatgcaccc ggggcgcaat ggacacaggc
      481 tgacgttgat aaatatctca atcataaaaa gatcactgac actgaagtgg ataatttcaa
      541 atcaactgaa atgtattcgt tatcggggac ggaggaagaa cagcttgaga aaatcatcaa
      601 ccagaaaatc gttgctctgt atccggacgt attcgaaggc tggtgcgagt acaggagaac
      661 cggttatccc aggttgccga taggcccgga tgaatcactg ctgaaaggcg tggtgccgag
      721 aagaaatcca tggccctata atgaaagaaa cattaatagt gaaagttaca atgaggctct
      781 cgccaggtat gggggcaagg ataaccgtct ggtcaaattc tggtgggata aaaatccgaa
      841 tgcaccgcat gtctatcagt ggacaccacc gtctatgcct agagcatggc agtaaaagga
      901 tagtcattta agaaaagctc atgaagcatg tgcttcatga gctttttctt tgattaagca
      961 tcagacttcc ccgctgattt caaagccttt cctgtaggat ttctctgaca gtcgcctggc
     1021 ttcttcgtca ttgacaatgg cctcatattc cggggagaaa agcaactgtt tgttccctgc
     1081 gcggtaggaa agattggcca gatggatcag taccgagctg ttgtgtcctt ccacaatact
     1141 gccgttgggc tgctttcttg tccttatgca gtcgatgtaa ttcaggatat gagccttgag
     1201 ggggaagatg cccttgtcct gtgcaacaat cttctcatcc ttgtcgaatg cctgccatcc
     1261 tcctcccatt ctcccggcta tcatcatttt ctttgtgccg taaatatcaa ttctggttgc
     1321 attctgcatc cacttcgggt agatgcttcc atatcttacc tcaggacctt ccttctggaa
     1381 ataaggtgtg cattcgcctg tttcaagggt caggacaaaa tttccgtaat cgaaggttgt
     1441 catctgataa tccggtattt ctctcccgtc atcgaaaaaa tatctgcctc ccgcacaaca
     1501 ggccgatttt ggaaaccccg gatcgccgag tatcagtctt gccatgtcca gctggtggat
     1561 tgatccgccc gaggttaccg gccccccgga ataatcccag taatatatcc aggatttgtt
     1621 cctcgataca ttatacggaa ctttcggtgc cggtcccagc cacatatccc aatcaatggt
     1681 atcgggtacg gcagaatctt ccttttcatt aaagggaaca gggccatcca gcagttcttt
     1741 cacatgtacc gccacaatat ccccaagctc accgcttttt atatagtcac gggcagtaag
     1801 tgcataatcg gcgcttctgt tttgcattcc gcactgtaca attctttcat atttcattgc
     1861 tgcctttatc atctgctgac cttcaaaaat cgaatgcgat actgtctttt caacatatac
     1921 atccttcccg gcctggcagg cccgtattgt tgcaagtgca tgccagtgtt cgggtgtctc
     1981 tataaagacg ccgtcaacct ccggatcgtc aaacacagtc cgcatgtccc tcacagacag
     2041 cggtctgaaa ccctggatct tttccagttc ggaaatggca cggcccccac gtgtatcgtc
     2101 aacatcacat acatatttta tcaatatatt ctcgcctgat gatgcagctt ccagtatcag
     2161 tgccgtgccc catgccccgg caccgataag ggcaagaatg accctgtctg atgccggcct
     2221 ggttttcgag gatggaaaat cactctttat cattgtcctg tagtcaggta ctatgttttt
     2281 cactgcagat tttccggaat gtgatcccct ttcaccggca gaatttctgc atgagcttcc
     2341 aagtgccata cctgctattc cggcagacgt tattgtgaaa aattttcttc ttttcatagt
     2401 ggaggaattt attgatttat ctgaggcttt atatactgta gtccggcagg gcgggaaatt
     2461 ctatggtatt cctttcactt ccggtactga ccgttttgca atatatctga aaggactttt
     2521 tgaatgttct gcgttcagca tttacgaaaa aggctttaaa agagtgttgc aggaatttta
     2581 aaaaagaagt aaaccttgct tccaggtgta aagaaaacat ttctttcttt tatatactta
     2641 atatctacaa aattatatta ttatttttat aattgtaagt gcgtatggtt ttccgaaaca
     2701 cttcctgttg ttttatccgg agccttttct tattttatcg tttgacaaaa gaggataaca
     2761 tttatatgaa atcacgcatt gtaatttttt cagacgatta aatatgtata aaagctgttt
     2821 gttccggcag gggatattta agatattctt taataaaaaa gcgtttgaat catttaatat
     2881 tttgaataat caatagttat gaaaaatctt ggaatctgca gtttcattct ttttctttcc
     2941 gttacagtcg gagcctgcag ggaacgcagt tcaggcaaat tcagcgggaa agacgggcag
     3001 caatatgatc agtggcatgt tataggaccg ggaggaggag gcggggtgtt caagccaacc
     3061 cttagtccgt tcaatgaaaa tttcgtgctg actcattgtg atatgacagg ggcatatctg
     3121 tcgcatgacg gcggccggac ctggaaaatg aaaaatctct ggaatgtgcc ccttgatttc
     3181 gagtttgacc cggcagattc aaataccgtt tatacagcta ctcagggatt tcttcacggg
     3241 gaagacaggg gttcaggaat ttctttactg ctccggtcct ccgacagcgg ggaaacatgg
     3301 gaaatatgct atcctgatat ttcatctgcc ggaaaggctg aaaagattca aagttcccga
     3361 ttcagaattt cagaactggt tgagggtgcc attgacggta atatacagaa agttgaagta
     3421 gatccggaag ataatgaatg tatttatctg ggtattgctc ccctctttga atttatggga
     3481 ggacggggct ctcaatcact tccttcagaa gtctacgttg tatcaacctc agacaggggc
     3541 aggacctggc gccggcttgc ctcgtttcca ggaacagaga ttcttgccct gtttcccggg
     3601 gcagacagtg aaaaactcat tgctttctca gacagggcct gtgttcatat tgacaaatcc
     3661 agcggcagca tgactgcttt tcccatgcca tcagaaagga ttattataat tgacggcgga
     3721 aaaagcaata atggatttct gatatacgca caggcgccgt ttacggctgg caggaaagga
     3781 gggatgttgt tgagcagcga cctggggaag tcctggcgtc agataaatga gggattgttg
     3841 tctgaaaacg aaaaagatcc gcttccttcc ttccggcagg ctcttgctgt gtgtgaaaac
     3901 cgggcggaaa cagtttatat aggcattgat ttcccccgga aggattccgt ggggcgcaat
     3961 gatccgcttt actgtattta caagacggag gatgccggaa aaaaatggga gccggtgctt
     4021 gtgtcttcgt cgcgcagggg ttatattacc ggtaatttca gcggttcatg gatggaggaa
     4081 agctatgatc ccggatgggg cggcagtccc attgatctgg gagtggcacc gaacaacccg
     4141 gatgtatgct atgcaggtga taacgggaaa ggatacagaa cagcagacgg aggcaaaaca
     4201 tgggtgcagg tatacagcca taacaacccc gacggttcat atgccagcaa cggtcttaat
     4261 gtgacaacat gctacggggt acatttcgat ccttatgaca gggatcattt ctttatcagc
     4321 tatacggaca taggcttgtt tcacacattc agcggcggaa aaagctggta tcactcgctt
     4381 gaaggaattc cacgcagctg gcaaaacacc tgctacgata tagaatttga ccccggtgtc
     4441 aggggaaggg tttggtctgc ctgggcaaat gcccatgacc tgccgcggac taaaatgttc
     4501 gggggccgtg gcttcggcgg gtatgaagga ggggttgcgg tttctgagga cggaggtctg
     4561 acatggaaaa aaagcagtga aggactcccc ggaaattcaa tctgtacgaa tgtgctgctt
     4621 gatccggcat cgccggaaaa caaccggact ctgtatgtca gtgtatttga caggggagtg
     4681 tataagaccg aagatgacgg taaaacctgg aatattgtaa acaatggttt tgggaaaaat
     4741 ctgtttgcct ggcagttaag acaaaacagc aggggacgcc tttttgccct cttcgcacgc
     4801 ggcgagtctg ccggtaaaac tgttgacggt gctgtttatt acagtgatga tagagcagca
     4861 acgtggacac agctgaaatt accggagaat gtcaacggac ctcatgacct tcttgtggat
     4921 ccactaaatc cggatattat gtatgtttcc tgctggccca gaagcgatct ggggccggat
     4981 gcaaagggag gggtcataaa aacaacagac ggcggaaaaa gctggacccg ggtatttgat
     5041 gaaaggatcc gtgttaactc agcaggcatg gatccccgga atcctgaaat aatctacatt
     5101 aatacatttc agaatgcagc ctaccgttca gaggattcgg gtgaaacctg gcagagaatc
     5161 gaagggtaca gattcaagtg gggtcagcga ccagttccgg atgtaaataa tcccggcatg
     5221 ctgtatctta caacatatgg ggggagtgtt ttccatggtc ctgcaaaagg aattcccgga
     5281 atccctgacg atatcacaaa tatgccagag ggctggtggt agaaattttt gcaacaggaa
     5341 tagaaccggt atgcccggga tgtccggctg aaaaaactcc ccggcaaa
//
LOCUS       DAOSPX010000222         5387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_13875_length_5387_cov_4.683046, whole genome shotgun sequence.
ACCESSION   DAOSPX010000222 DAOSPX010000000
VERSION     DAOSPX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13875_length_5387_cov_4.683046"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="PLZ75_10250"
     CDS             <1..1780
                     /locus_tag="PLZ75_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOS72812.1"
                     /translation="AAALKAGTDLECGVDYSELMTALERGYVSDADIDLAAKRVFTAR
                     FKLGMFDPDDMVPYAQIPYFVNCSDYNNGLSRTAAQKSIVLLKNENNILPLDREKIKT
                     LAVIGPNADNFESLIGNYNGIPKDPVTVLRGIRNKLEPAAQVIYAEGSDLAEGIHNLS
                     VIPSCYLETPDGRQGVLGEYFDNRDLEGEPVFIRTDDQVNFYWEYVSPRYDLPAGNFG
                     VRWTTYLVPPESGKYVIGGWGSPAYEIFLDGKRILRERSEHHAFHKEYSAELQAGRKY
                     KIEILFRNYDRDADMKLLWTPPRPDMVGEAVRAAGEADAVVLVLGLSQRLEGEEMSIS
                     IDGFAGGDRTNLNLPAVQEELFDALAATGRPVIVVLMSGGALAVNNVQQKAAAVLLAG
                     YPGQQGGNAVADVLFGDYNPAGRLPVTYYKSVDQIPAFENYDMAGKTYRFFTQEPLYP
                     FGYGLSYTTFSYSDLSMPERIPAGEKVKVSVKVTNTGKTDGDEVVQLYLTDEKASTPR
                     PVRQLEGFTRITLKAGETKDVEFTLEPRQFSIINRKEKRVIEPGWFTISVGGKQPGFK
                     GYLDPQFTRVLSGRLRLTGRETAIAD"
     gene            complement(1878..3158)
                     /locus_tag="PLZ75_10255"
     CDS             complement(1878..3158)
                     /locus_tag="PLZ75_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS72813.1"
                     /translation="MRKILVSAVLISAMLLNLHSQAGTQLIKVIVAPDHQDWTYKVNE
                     KAKFTVLVFKYGNLLENVTVDYEAGPDMLPDIKKEGVVLKNGKLELSGTMKQPGFYRV
                     RVWAVADGKRYEGLATAAFEPEKIQPTVKDPSGFDSFWNDEIAKARKINLDPRLTLVP
                     ERCTSDADVYHISFQNEAPGSRIYGMLAMPKKPGKYPAILRVPGAGIRPYSGDTRTAG
                     QGFIVLDIGIHGIPVNLEQQLYTDLGGAALSEYFNIRMNDRNRHYFKRVYLGCVRAVD
                     FIFSLPEFNGTDIAVTGGSQGGALTIVTAGLDKRIRFAAGLYPALCDHTGYLNKRAGG
                     WPHYFRNTEPKPGEVETLAYFDVVNFARRVSAPGYYTWGFNDIVCPPTSMYSAYNVMT
                     APKELHLYQETGHWTFPEQTEALNNWLMKKLKGE"
     gene            3428..4885
                     /locus_tag="PLZ75_10260"
     CDS             3428..4885
                     /locus_tag="PLZ75_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOS72814.1"
                     /translation="MSSVNHLLQKRVNLFDGISIVAGGMIGSGIFIVSADIARTVGSP
                     GWLLVVWLITGILTIIGAMSYGELASMMPDVGGQYVYLKEAYHPLAGFLFGWTTFLVV
                     QCGSIAAVAVAFAKFSGVLLPWISESNILVDAGLLKINTTMVVAIAMIAFLTWLNTRG
                     IVTGKTVQNIFSSTKVIALAGFILIGLFALRSTNSLEVNREIFWKASQTGADLQSTPL
                     AGWALIAAIGTALVGSLFSSDAWYNVTYISGETINPKRTIPLSLFYGTLLVSLIYLLT
                     NFIYISILPVMGSPDGTDVFSRGIQFATDDRVATSAMFAILGDYAAIIMALFIMISTF
                     GCNHSLILTAPRVYYAMAQDGLFFRKAGEINRFGVPGFALVVQGIWSILLCLSGTYSD
                     LLDYVIFAVLIFFAITTLAIFVLRVRRPDIPRPYKAFGYPVIPAVYILATVVIMVILL
                     IYKPKYTFPGLIIVLLGVPVYYLWKKYSGRQNSHQ"
     gene            4890..>5387
                     /locus_tag="PLZ75_10265"
     CDS             4890..>5387
                     /locus_tag="PLZ75_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HOS72815.1"
                     /translation="MFPDAFRKRIEKQEYIDSGQLLAALGEESPVSIRINRDKWEGVP
                     LNSAPVPWCSDGYYLDSRPSFTFDPLFHAGCYYPQEASGMILGEIFRQISSAGEYARI
                     LDLCGAPGGKSTHLSSLAGEDRLLVSNEAIRSRAAVLAENLSKWGIPNVIVTQGDPSA
                     FGRLTG"
BASE COUNT         1348 a         1234 c         1327 g         1478 t
ORIGIN      
        1 ggcagcagca ctgaaggcag gtactgatct tgaatgcggt gtggattaca gcgagctgat
       61 gaccgccctg gaacggggat atgtttccga tgcggatatt gatcttgcag cgaaaagagt
      121 ttttacagca cggtttaaac tcgggatgtt tgatccggat gacatggttc cctatgcgca
      181 gataccatat tttgtaaact gttcagacta caataacggt ctctcgcgga cagccgcaca
      241 gaaatcaatt gtacttctga agaatgagaa caatattctg cccctggaca gggagaagat
      301 aaaaaccctg gcagtgatcg ggcccaatgc cgataatttt gaatctctca ttgggaacta
      361 caatggcatc ccgaaagatc ccgtaacggt tctgagaggt atcagaaaca agctggagcc
      421 tgccgcacag gtcatttatg ccgaaggaag tgatcttgcc gaaggaatac ataatctttc
      481 agttattccc tcctgttatc tcgagacacc tgacggcaga cagggtgtcc tgggggagta
      541 tttcgacaac agggatctgg aaggcgagcc tgtttttatt aggacagacg accaggtaaa
      601 cttttactgg gagtatgttt ccccgcgata tgatcttccg gccggaaatt tcggggtaag
      661 atggactaca tatcttgttc cccccgaatc cgggaaatat gttatcggag gatggggatc
      721 accagcctat gaaatatttc ttgacggcaa aaggatactg cgggaaagaa gtgaacacca
      781 tgctttccac aaggaatatt ctgctgaact tcaggcaggc aggaaataca agattgagat
      841 attgttcagg aattatgaca gggatgccga tatgaagctg ttatggactc cgccacgccc
      901 ggacatggtc ggggaagcgg taagggcagc cggggaggct gatgctgttg tgcttgtcct
      961 gggtttgtca cagcggcttg aaggggagga gatgagtatc agtattgatg gttttgccgg
     1021 gggagacagg acaaacctca atctgccggc tgttcaggaa gagttgtttg atgccctggc
     1081 agcgacgggc aggccggtca ttgtggtact aatgagcgga ggagctctcg ctgtcaataa
     1141 tgtgcagcaa aaggcagcag ctgtcttgct ggcaggctat cccggacagc agggaggaaa
     1201 tgcggttgca gatgtattgt ttggcgacta caatcctgcc gggagacttc ccgttacata
     1261 ttacaaatcg gttgatcaga tacctgcttt tgaaaactat gacatggccg gaaaaaccta
     1321 caggtttttt acacaggaac ctttgtatcc ctttggatat ggtctgagtt acacaacgtt
     1381 cagctatagt gatctcagta tgccggaaag aattcctgcc ggcgagaagg taaaggtctc
     1441 tgttaaggtc accaataccg ggaaaactga cggggatgaa gtggtacagc tgtatctgac
     1501 tgatgagaaa gcttcgactc ccaggcctgt acgccagctg gaaggtttta ccaggataac
     1561 cctgaaggcg ggtgaaacaa aagatgttga attcaccctt gagccccggc agttttcaat
     1621 tattaaccgg aaagaaaaaa gagtgattga gccgggatgg tttaccatat ccgtgggagg
     1681 caagcaaccg ggattcaaag gttatcttga tccgcagttt acccgggttc tctcgggcag
     1741 gctcaggctc accggcaggg aaacagcaat tgcggattaa ccttccggcc ggcagaatgt
     1801 tattgccatg atccgtaccg taagtaaact tacggtacgg tgccggccgg cagtggactt
     1861 ttcagggagg aaattttcta ttctcccttc agttttttca taagccagtt attcagggct
     1921 tcggtctgct cggggaaagt ccagtgtcct gtttcctggt agagatgcag ttcctttggt
     1981 gccgtcatca cgttatatgc cgaatacatg gatgttggcg gacatactat gtcattgaat
     2041 ccccaggtat aatatccggg tgcactgacc cttcttgcaa aattcaccac atcaaaatag
     2101 gcgagtgttt caacctcccc gggttttggt tccgtatttc tgaaatagtg aggccatccg
     2161 cccgctcttt tgttaagata gcccgtatgg tcgcaaaggg ccggatagag accggcggca
     2221 aatctgatac gcttatcaag cccggcagtg actatggtaa gggcgccacc ctggcttccg
     2281 cccgttaccg caatatccgt tccattaaat tccggaagtg agaaaataaa atcaactgcc
     2341 cttacacatc cgagatacac tcttttaaag taatgcctgt tcctgtcgtt catcctgatg
     2401 ttgaaatatt ctgaaagggc tgctcctcca aggtcggtgt ataactgctg ctccaggttc
     2461 accggtattc cgtgtattcc gatatcaaga actatgaacc cctgcccggc ggttctggta
     2521 tctcccgaat aaggccttat tccggctccg ggaactctca gtattgcagg atatttcccc
     2581 ggttttttag gcatggcaag cattccgtat atcctcgatc ccggagcttc attctgaaag
     2641 cttatgtgat agacatctgc atcggaggtg cagcgttcgg gaacaagggt gagccgcggg
     2701 tcaagattaa ttttccgggc ttttgcaatt tcatcattcc agaaactatc gaaaccggaa
     2761 ggatccttaa cggtaggctg tatcttttcc ggttcaaacg cagcagtggc cagaccttca
     2821 tatcttttgc cgtcagcaac agcccagacc ctgacacggt aaaacccggg ctgcttcatt
     2881 gttccgctga gttccagctt tccgtttttc agaaccactc cctccttctt tatgtcaggc
     2941 agcatatccg gacctgcttc ataatcaaca gtaacatttt caagaagatt tccatacttg
     3001 aaaaccagta cggtgaactt cgctttttca tttaccttgt atgtccagtc ctgatgatca
     3061 ggagccacaa ttacctttat cagctgtgtt ccggcctggg aatgcaggtt cagcaacatg
     3121 gctgaaatta aaaccgcaga tactagaatt tttctcatgt ttgaattatt ttattaaaca
     3181 aatgatattc agtcagataa caaatttgct gcatttaaat agtatatatt tcaatattat
     3241 acttttaaag gattcttcag gaatttataa aatgaaacat ggtccgccat tctaaaaatc
     3301 ttaatgttgg tgtttgagga ggaagaatat ttcatggttt aaagaattcc gacagtctaa
     3361 aaataaacat atttgaattt aaattcaatt gtttaattat ttttgtcggc atcctacaaa
     3421 ccaacttatg tcatcagtca accatcttct gcaaaaacga gtaaaccttt tcgacggaat
     3481 ttcgatagtt gccggtggca tgatcggctc gggaatattt attgtaagtg cggatattgc
     3541 ccggacggta gggtcgcccg gctggcttct tgtagtctgg ctgataaccg gcatccttac
     3601 catcatcggg gcaatgagct atggtgaact ggcatccatg atgcccgatg tgggcggtca
     3661 gtatgtgtat ttgaaggaag cttaccatcc tcttgccgga tttctgttcg ggtggacaac
     3721 ctttcttgtg gtccagtgcg gatcgatagc tgcggttgca gttgcatttg caaagttcag
     3781 cggtgttctc ctgccatgga tatcagaatc caatattctg gtggatgcag gactgctgaa
     3841 gataaatacc acaatggttg tggccatcgc catgatagct tttcttacat ggcttaacac
     3901 caggggaata gttaccggga agacagtcca aaatatcttt tcttcaacaa aggtcatcgc
     3961 ccttgcaggt tttatcctga tcggactctt tgccctcaga agcactaatt cacttgaagt
     4021 gaacagggag attttctgga aagcgagtca gactggcgca gatctgcaga gtactccgct
     4081 tgcaggatgg gcccttatag ctgctattgg aaccgctctt gtcggatctc ttttttcttc
     4141 ggatgcctgg tataatgtta cctatatatc gggagaaacc atcaatccca aacggaccat
     4201 tccactgagc ctgttttacg gaaccctgct ggtttccctc atctaccttc ttacaaattt
     4261 tatttatata agcattcttc cggtaatggg ttctcctgac ggaacagatg tattcagcag
     4321 ggggattcag ttcgccactg acgacagagt ggccacgtcg gcaatgtttg ccatacttgg
     4381 tgattatgcg gcaatcataa tggccctgtt cataatgatc agcaccttcg gatgcaatca
     4441 cagccttatt ctcacggcac cccgtgttta ttacgcaatg gcacaggatg gactgttttt
     4501 caggaaggcc ggggaaatca accgtttcgg ggttccgggc tttgcactgg tggttcaggg
     4561 aatatggtca atattactct gtttgtcggg cacctacagc gaccttcttg actatgtgat
     4621 cttcgcggtt ctgattttct tcgccattac caccctggcc atttttgtcc tgagagtcag
     4681 acggccggat atcccccgtc catacaaggc tttcggctat cctgtcattc cggctgttta
     4741 catactggcc acagtagtta taatggtgat cctcctgata tacaagccga aatacacatt
     4801 ccccggactt ataattgtat tgctcggcgt tccggtgtat tatctgtgga agaagtattc
     4861 cggcaggcaa aattctcatc agtgaaatca tgtttccgga tgctttcagg aaaagaattg
     4921 agaagcaaga atatattgac agcggacaac ttctggctgc actgggggaa gaatcccccg
     4981 tttcaatccg tataaaccgc gacaaatggg aaggtgttcc tttgaattcc gctcccgtac
     5041 cctggtgcag tgacggatac tatctcgaca gccgtccctc attcaccttt gatcccctct
     5101 tccacgccgg atgctattat ccccaggaag catcgggaat gattctcggg gagatattca
     5161 ggcagatatc cagtgccggt gagtatgcca ggattcttga tctctgtggt gctcccggag
     5221 gtaaaagcac tcatctctca tcgcttgcgg gtgaagacag gctgcttgta tccaatgaag
     5281 ctatcaggag cagggcggcg gttcttgcgg agaatcttag caaatggggt attccgaatg
     5341 ttatcgttac acagggtgat ccttccgcat ttggccgcct tacaggc
//
LOCUS       DAOSPX010000223         5326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14081_length_5326_cov_4.828496, whole genome shotgun sequence.
ACCESSION   DAOSPX010000223 DAOSPX010000000
VERSION     DAOSPX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14081_length_5326_cov_4.828496"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLZ75_10270"
     CDS             complement(<1..197)
                     /locus_tag="PLZ75_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOS72816.1"
                     /translation="MLKKRIIPCLDIRDGRTVKGVRFVDIRDAGDPLELAVRHASGGA
                     DELVLLNISPAGENRKTFAKCV"
     gene            complement(191..907)
                     /gene="hisA"
                     /locus_tag="PLZ75_10275"
     CDS             complement(191..907)
                     /gene="hisA"
                     /locus_tag="PLZ75_10275"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOS72817.1"
                     /translation="MMRIIIAIDIIDGKCVRLTRGDFDTKKVYNEDPLEIARMLEDNG
                     IKYIHLVDLDGAREKKIINHRTLEKIAGRTGLIIDFGGGIRSDDDVRAAFDSGARQVT
                     AGSIAATDRDLFIRWLLHYGNDSIILGADFRERKISVSGWTETLNIDITDYISDYYEK
                     GVKYTICTDAERDGMLEGPATDVYREILEKTGISLIASGGVSELKDLDELRDIGCEAA
                     IIGKAVYEGRINLKDLRNLC"
     gene            complement(901..1482)
                     /gene="hisH"
                     /locus_tag="PLZ75_10280"
     CDS             complement(901..1482)
                     /gene="hisH"
                     /locus_tag="PLZ75_10280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOS72818.1"
                     /translation="MMIAIVKYNTGNNLSLSNALTRLGYDNIITDNGEEIINASKVIL
                     PAGGEAAPAMKHLRERELDKLIPPLRQPVLGICLGFQLMCRYTEEAKINGLEIFNCSV
                     RMFPKYDRLPHMGWNDFISVWGDIFRGISRKDDVYYAHSHYAGICSNTTAICDYILPF
                     SAAMKKDNFYGVQFLPEKSAATGETILRNFLEL"
     gene            complement(1476..2576)
                     /gene="hisB"
                     /locus_tag="PLZ75_10285"
     CDS             complement(1476..2576)
                     /gene="hisB"
                     /locus_tag="PLZ75_10285"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOS72819.1"
                     /translation="MKKVLFIDRDGTIIVEPEDEQIDSLEKLRFLPGAISSLSAIAKT
                     TDYELVMVTNQDGLGTSSFPEDTFWPAHNKMLQILGDEGVCFSEIFIDRTFPEENAPT
                     RKPGTAMLTRYLARGIDPENSYVIGDRLTDLQLAENIGCGAILIRKPSGPDPASGKVF
                     STGDWNEIFRYLTSKPRTASFSRKTKETDISLHLNIDGSGKSSISTGIGFFDHMLDQI
                     ARHAGFDLSVRAKGDLEVDAHHLVEDTAICLGETFNNALGSKKGIARYSFVLPMDDCL
                     AQVAADLGGRPWLVWDVQFRGSHIGEMPSEMVFHFFKSFSDSAKCNLNIKAEGTNDHH
                     KAEAVFKAFARTLGSAAGKTGNHDLPSTKGTL"
     gene            complement(2573..3601)
                     /gene="hisC"
                     /locus_tag="PLZ75_10290"
     CDS             complement(2573..3601)
                     /gene="hisC"
                     /locus_tag="PLZ75_10290"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOS72820.1"
                     /translation="MFNLDDLVRPNVKRLSPYSCARDDFHGRAGVFLDANENPYGTLN
                     RYPDPYQRELKDRISRLKSVPVENIFLGNGSDEVIDLCFRIFCNPGQDKVLTLYPSYG
                     MYEVSASVNDVEIIRSELTPEFGISYSDVKKHFSDRNLRLIILCSPNNPTGNCLDNET
                     VERIISEFRGIVLLDEAYIDFAAQPSMKDKISQYPNLIVMQTFSKAFGLASVRIGMAF
                     TNAEIAGYLNKIKPPYNISSLNQRAVLRRLEKKESHRSQVTRIREERTRLSGEIAKLS
                     CVVEVYPSDANFILTRMKDAAKIYEYLVNKGIIVRNRSSLINNCLRITVGSATENNKL
                     LRALKNYK"
     gene            complement(3621..4907)
                     /gene="hisD"
                     /locus_tag="PLZ75_10295"
     CDS             complement(3621..4907)
                     /gene="hisD"
                     /locus_tag="PLZ75_10295"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006846638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOS72821.1"
                     /translation="MRIFKNPPRETWEDLCRRPLADPAELDSEVKAILGKVKKQGDSA
                     LRYYSAEFDHISIIDFKITERELDEAEKFVSDDLKNAIRIASGNIYRFHKSQLRDEET
                     VETMPGVKCWRRNVAIDKVGLYIPGGSAPLFSSVLMLGIPAKIAGCRRVIICTPPGRQ
                     GKINPLILYAAKITGISEIFRIGGAQAIAAMAYGTETIPRVDKIFGPGNQYVTKAKEI
                     AASEGTAVDMPAGPSEVLVIAGPQADPTFIAADLISQAEHGPDSQVVLLTDCEKLLSE
                     VSNELSVQLERLPRKDIAALSLDKSMAILLKDPYECIDFSNEYAPEHLIINTDDPHEL
                     AGKVISAGSVFLGKYSCESAGDYASGTNHTLPTNRAAKSYSGISTDSFLKKITFQELS
                     REGIEKLGPVIEVMAGAENLAGHAEAVTIRLNSLRQ"
     gene            complement(4907..>5326)
                     /locus_tag="PLZ75_10300"
     CDS             complement(4907..>5326)
                     /locus_tag="PLZ75_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOS72822.1"
                     /translation="PGIGLADAICDIVSSGSTLDTNGLREVITILDSQAVIIGNKDLD
                     DEKKAILAKLLFRIRAVKRSNENKYILLNAPESAVGEICRILPGIKSPTILPLAEKGW
                     CSLHSVVREDDFWERIDQLRKAGAEGILVIPIEKMIL"
BASE COUNT         1169 a         1363 c         1208 g         1586 t
ORIGIN      
        1 acacactttg caaaggtttt gcggttttca cctgccggac ttatattcag aagcaccagt
       61 tcatcagctc cccccgaagc atgcctgact gccagttcaa ggggatctcc cgcatccctg
      121 atgtcaacga accgtacacc ctttacagtt ctgccgtcgc gtatatcgag gcagggtatt
      181 attcttttct tcagcatagg ttcctcagat cctttaagtt aattcttcct tcataaacag
      241 cctttcctat tattgcagct tcacatccaa tatccctcaa ttcatccaga tccttcaatt
      301 cagagacacc cccgctggca ataaggctga tgcctgtctt ttccaggatc tccctgtata
      361 catccgttgc cggtccttcc agcatcccgt ccctttccgc atcagtgcat atcgtatatt
      421 tcaccccttt ttcataatag tcggatatat agtctgttat gtcaatattc agggtctctg
      481 tccagccgct tacagaaatc ttcctttccc tgaaatcagc tcccagtatg atactgtcat
      541 tcccgtaatg gagcagccag cggatgaaca aatccctgtc cgtggccgca atacttccgg
      601 ctgtcacctg ccttgccccg gaatcgaagg cagccctgac gtcatcatcc gatcttattc
      661 ctcctccgaa atcaatgatg agtccagtcc tgcctgctat cttctccagt gtcctgtggt
      721 taatgatctt tttctccctt gccccgtcga ggtcaacaag gtgaatatat tttatcccgt
      781 tgtcttcaag catccgggca atctcaaggg gatcttcatt gtacaccttt tttgtgtcga
      841 aatcccccct ggtcagtctt acacatttac cgtcaatgat gtctatggca ataattattc
      901 tcataattcc agaaaatttc tcaatatcgt ttcgcctgtg gctgcagatt tttcggggag
      961 aaactgcaca ccgtagaaat tgtctttttt cattgcagcg ctgaagggaa ggatatagtc
     1021 gcatattgcc gttgtgttgg aacagatgcc cgcatagtgg ctgtgagcat aatacacatc
     1081 atccttccgg gaaattcccc tgaaaatgtc gccccataca gaaataaaat cattccatcc
     1141 catatggggg agcctgtcat atttcggaaa cattctgacg ctgcaattaa aaatctcaag
     1201 tccgtttatt tttgcttctt ccgtatatct gcacatcagc tgaaacccga ggcatatacc
     1261 cagtacgggt tgcctgaggg gagggatgag tttgtccagt tccctttccc tgagatgttt
     1321 cattgccgga gcagcttcac ccccggcggg caggatgact ttggatgcat ttatgatctc
     1381 ttccccgttg tctgttatga tgttgtcata tcccagcctt gtcagtgcat tactgagtga
     1441 aaggttgttg ccggtattat attttactat ggcgatcata atgtaccctt ggtggaaggc
     1501 aggtcatgat ttccggtttt ccctgcggct gatccgagtg tcctggcaaa tgccttgaat
     1561 acggcttctg ccttgtgatg atcatttgtt ccttcagctt ttatattgag attacatttc
     1621 gcgctgtcgc tgaatgattt aaaaaaatga aaaaccatct ccgagggcat ttcacctata
     1681 tggctgcccc ggaattgcac atcccacacc agccatggcc tgccgccaag atcagctgcg
     1741 acctgtgcca gacagtcatc catgggcaga acgaagctgt acctggcaat tcccttcttg
     1801 ctgcccaggg cattgttaaa tgtttcccca aggcagattg ccgtatcctc aacaagatga
     1861 tgagcgtcca cttcaagatc tccttttgcc cgtacggaaa ggtcaaaccc cgcgtgccgg
     1921 gctatctgat caagcatgtg atcaaaaaag cctattccgg tgctaatgct gcttttaccg
     1981 gaaccgtcga tattaaggtg aaggctgata tcggtttcct tcgtttttct ggaaaaggag
     2041 gcagtccgtg gttttgatgt gagatatctg aagatctcat tccagtcgcc ggtggaaaat
     2101 actttgcctg aagccgggtc aggaccggaa ggcttacgga tgaggattgc cccgcagccg
     2161 atattttccg caagctggag atcggtcagc ctgtcgccta tcacatagga attttccggg
     2221 tcgatgcccc gggcgagata cctggtcagc atagctgtgc caggcttcct tgtcggtgcg
     2281 ttttcttcgg gaaaggtcct gtcgatgaag atttccgaaa agcagacacc ctcgtccccg
     2341 agtatctgaa gcatcttgtt gtgtgcaggc cagaaagtat cttcaggaaa tgaagaagtg
     2401 cccagaccat cctggtttgt caccataacc agctcgtaat cagtagtctt tgctattgct
     2461 gaaagactgc ttatggcccc gggaaggaat cttaattttt ccagggaatc aatctgttca
     2521 tcctccggtt ccacaatgat ggttccgtcc ctgtctataa aaagcacctt tttcatttat
     2581 aattttttag agctcttaaa agtttgttgt tctctgtcgc ggaaccaact gtgatcctga
     2641 ggcagttatt gatcagactg ctcctgtttc gtactataat acccttgttt acaagatatt
     2701 catatatttt cgcggcatcc ttcatccttg tcagaataaa atttgcatct gagggataga
     2761 cttccacaac gcaggacaat ttagcaattt cccctgaaag tctggttctt tcttccctga
     2821 ttctggttac ctgcgatctg tggctttcct ttttttccag ccgtctgagg accgctctct
     2881 ggttcaggga gcttatattg tagggaggtt tgatcttgtt gagatagcct gcaatttccg
     2941 cattggtgaa ggccattcct attctgacgg atgcaaggcc gaatgccttg ctgaatgtct
     3001 gcatcacgat gagattggga tactggctga ttttatcctt cattgaaggc tgagcggcaa
     3061 aatcgatgta ggcctcgtca agcagcacaa ttcccctgaa ttccgaaatg atcctttcga
     3121 ccgtctcatt gtccaggcag tttccggtgg gattattggg ggagcacagt attataagcc
     3181 tgaggttcct gtcgctgaaa tgttttttca catcgctgta gctgattccg aattcagggg
     3241 tgagttcaga cctgattatc tccacatcat tcaccgaggc tgatacctcg tacatgccgt
     3301 aagaggggta gagggtaaga accttgtcct gtccgggatt acagaagatt ctgaaacaca
     3361 gatctatcac ctcatcactg ccgtttccca ggaaaatatt ttccaccgga acggatttca
     3421 gtcggcttat ccggtctttc agttcacgct gatacgggtc ggggtacctg ttcagcgttc
     3481 cgtagggatt ttcgttggca tcaagaaata cgcccgccct gccgtgaaaa tcatcccgtg
     3541 cacaggaata gggactgagc ctttttacat tcggccttac aaggtcatca agattgaaca
     3601 taaaacagaa ctttcatgga ttattgtctt aagctgttga gcctgatggt tacggcttcc
     3661 gcatgaccgg caaggttttc tgccccggcc atgacctcta tcaccggacc cagtttctct
     3721 attccttccc ggcttaactc ctggaaggtg attttcttca aaaaactatc ggttgaaatc
     3781 ccgctgtaac tctttgccgc ccggttggtt gggagagtgt gattggttcc cgaagcatag
     3841 tctcccgcgc tttcacagct gtattttccc aggaagaccg agccggcgct gatcactttc
     3901 ccggcgagtt catgaggatc atccgtattg ataataagat gctcgggagc atattcattg
     3961 ctgaaatcaa tgcattcata cgggtctttc aggagtattg ccatgctctt gtccagcgac
     4021 agggcggcga tatcctttct ggggagtctt tcaagctgta cggacagctc attgctgact
     4081 tccgacagca gtttttcgca gtcggtcagc agtacaacct ggctgtcggg tccgtgttcc
     4141 gcctgtgaga tgaggtcggc ggctatgaat gtcggatcgg cctgcgggcc ggcaatgaca
     4201 agaacctcac tcgggccggc gggcatgtca acggctgttc cttccgaggc tgctatttcc
     4261 ttggcttttg tgacatactg gttgcctgga ccgaatatct tgtcgacgcg tggtatcgtt
     4321 tccgttccgt aggccattgc tgctatggcc tgtgcgcccc cgatcctgaa tatttcactt
     4381 atacccgtga tttttgcggc atagagtatc aggggattta tttttccctg cctgccgggg
     4441 ggagtgcata ttattacccg tctgcagccg gcgatttttg caggtattcc gagcatcagc
     4501 acgcttgaaa agagcggagc gctgcccccc ggaatatata atccaacctt atcgattgcc
     4561 acattcctgc gccagcattt cacacccggc attgtttcaa cggtttcttc atccctgagc
     4621 tgtgacttgt gaaacctgta tatgtttccg gaggcgatcc tgattgcatt tttcagatca
     4681 tcgcttacga atttttctgc ttcatccagt tccctttcag tgattttgaa atcgatgatg
     4741 ctgatgtggt caaactctgc ggaataatat ctcagtgcac tgtcgccctg ttttttcacc
     4801 tttccgagaa tggctttcac ctccgaatcc aattctgccg ggtcagcaag aggtcttctg
     4861 caaaggtctt cccatgtttc cctgggcggg tttttaaaga ttctcatcag agtatcattt
     4921 tttcaattgg tatcacgagt attccctcgg caccggcttt tctgagctga tcaatccttt
     4981 cccagaaatc atcctcccgt acaacggaat ggagtgagca ccagcctttt tctgcaaggg
     5041 gaagaattgt cggactcttt attcccggga ggatgcggca tatctccccg actgcagatt
     5101 ccggggcatt caggagaata tacttgtttt catttgatct tttcacggcc ctgattctga
     5161 aaaggagctt tgcgagaatg gctttttttt cgtcatcaag gtccttgttg ccgattatca
     5221 cagcctgact gtcgagtatt gttatcacct cgcgaagccc gttcgtgtcc agggtgcttc
     5281 ctgaactcac aatatcgcat attgcatccg cgaggcctat tcccgg
//
LOCUS       DAOSPX010000224         5313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14128_length_5313_cov_2.856599, whole genome shotgun sequence.
ACCESSION   DAOSPX010000224 DAOSPX010000000
VERSION     DAOSPX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14128_length_5313_cov_2.856599"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLZ75_10305"
     CDS             <1..418
                     /locus_tag="PLZ75_10305"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72823.1"
                     /translation="RAPVLWQHRLWGNGDVTREFNNGIFFHGEKGSLFAEDERLAIFP
                     AGREGKREDISVPAPLMQENHVWNFLDAVRKKNRNLISCDPEDAFHSTATVQLAMISY
                     YTGTKVKWDNDKKEIIGNPAASALLKRNYRGKYVHP"
     gene            complement(591..1574)
                     /locus_tag="PLZ75_10310"
     CDS             complement(591..1574)
                     /locus_tag="PLZ75_10310"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOS72824.1"
                     /translation="MEKRNERIRIFAGSSSRAFAGKICSYLGIEAGISEVFQFSEGNT
                     YVKIGENVRNKDVYIVQTVGLKANDSFMELLFWIDSFKRSSARSVTVIMPYFSYAKAD
                     KKDEPRVSIRARVCADCIEIAGADRVITIDLHSPQIQGFFKKPVDNLYAYPLLCEYMK
                     SRGTADLVVVSPDAGFARDARRYGSYLGVPVYICEKTRTDHSERASVNILSGPLAAKN
                     ALIVDDFTISGGTLIETANLMKEKGVDQVYAMVTHALLHEKAIEELMKSPIHRLIATD
                     TVENPNLLNNEKIEIISVAPLFAEAVNIIQHEGSLSSLFDSLPERVLKSSF"
     gene            1959..2249
                     /locus_tag="PLZ75_10315"
     CDS             1959..2249
                     /locus_tag="PLZ75_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72825.1"
                     /translation="MELKELTVEKLTDQCRDLDRKYELLRAEYEQSKDLYSAMPSFEN
                     HEKLHDVHDRLIRCLEELSDSQNKLIEAYWSYFGVRHWQFKAIKDGSGIMRA"
     gene            2509..3243
                     /locus_tag="PLZ75_10320"
     CDS             2509..3243
                     /locus_tag="PLZ75_10320"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOS72826.1"
                     /translation="MDKKDLEQTLVLIKPDALKNSLTGYILSVLSEFHTGLHYCGTKI
                     VHVGRMLAEEHYAEHRGKVFFPAILEYLMGESHYPDNPMKRRVIAIVYQGRDAIKKIR
                     EVVGPTNPLNARVEKPGSIRSLGTMVPLKDDDGNVIGQRMDNLIHASDTPQSAEREIK
                     LWFRPDDMPPLMHTYPTEASGEFYYFRDNRLFREYEKGSVCILAPADVAWKSDLEILH
                     NISRGLPAAGSLQSVVAKYLINSATE"
     gene            complement(3272..4666)
                     /gene="mnmE"
                     /locus_tag="PLZ75_10325"
     CDS             complement(3272..4666)
                     /gene="mnmE"
                     /locus_tag="PLZ75_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOS72827.1"
                     /translation="MSETTVCAPATSGGGAIAIIRISGPQSIEICERIFVPADPKRRL
                     KEQKGFTIVLGDIKSGDEIIDEVLISIFRAPHSFTGEDSAEISCHASPYIQQKIMELL
                     ISHGAVAARPGEFTQRAFLNGRIDLSRAEAIADLVASESKMAHRIAMKQMKGSFSDEI
                     AVLRADLLHFASLIELELDFGEEDVEFADRKSLFEIVDRVLNVSRKLSSSFSLGNAVK
                     NGIPVVIAGNPNTGKSTLLNRLLMEERAIVSDIPGTTRDAIEDTVVIEGIRFRFIDTA
                     GLRDTTDIIENLGIKKTHEKISAASVILLLADINEGAGPLNALLKKIRGQLTDEKRIV
                     ILVNKTDNDLYGNRQLMQENILPKQDESMLFISAKTGEGIDELKKLLGRLAGAGRLNT
                     ENVIISNVRHYEALLHVAESLERVRTGLENNLQEDFIATDIRHAIHYLGEITGEITTD
                     EILQNIFRNFCIGK"
     gene            complement(4732..>5313)
                     /locus_tag="PLZ75_10330"
     CDS             complement(4732..>5313)
                     /locus_tag="PLZ75_10330"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOS72828.1"
                     /translation="TKSYPDGLEKRFIRFVEPADVRGTSMLVIDNKDKADEMWIYLPA
                     LKKTRRIVSGEKGKSFMSSEFSNADMSSPTLSDFTHRHTGSSGTANTWVIESIPKNDD
                     IVYEYGYSKKITYVCMDKFQVQKMEFYNFDNDLFKIIETKSVQSFQDGKYMIKNMIAS
                     NQITGRKSEILFSNINDKVTVQDSFFTVQNLER"
BASE COUNT         1419 a         1201 c         1148 g         1545 t
ORIGIN      
        1 ccgtgccccg gtattgtggc agcacaggct gtggggcaat ggcgatgtaa cacgggaatt
       61 caataacggt atcttttttc acggtgagaa aggcagcctg tttgcggaag atgaacgtct
      121 ggcaatattc cctgcaggga gggagggcaa aagggaggat atttccgttc cggcgcccct
      181 gatgcaggaa aatcatgtct ggaacttcct tgatgcagtc aggaagaaaa acagaaatct
      241 tatcagctgt gatcctgaag atgcatttca ttcaactgcc acagtgcaac tggccatgat
      301 atcctattat accggaacaa aggtgaaatg ggacaatgat aaaaaggaga tcatcggcaa
      361 tcccgcggca tctgcgctgc tgaagaggaa ttaccgtgga aaatacgtgc atccctgacc
      421 agggactgta tttaataagg aagtatttaa gcagaaattg attaacaagg cattaagtac
      481 attgcctgat aagggagagg ggagtggtaa ttcaggatac ggatccgttt tttattgagg
      541 agctgctttt ctgaggcagt tttcagatac tgtttttcag aagctacttt tcagaaactg
      601 cttttcagaa ctctttcggg aagcgaatca aacaatgaac tgagagagcc ttcatgctgg
      661 attatattca ctgcttccgc gaaaaggggg gcaactgaaa tgatctcaat tttctcgttg
      721 ttcagcagat tgggattttc aacagtatcg gtggcgataa gcctgtgaat cgggcttttc
      781 atcagttctt caattgcctt ttcatgcagc agggcatgcg tcaccattgc ataaacctgg
      841 tccacaccct tctctttcat caggtttgcc gtttcaatca atgttcctcc cgaaatggta
      901 aaatcatcca ctatcagtgc atttttcgcc gccagcgggc ctgaaagaat gtttacgctt
      961 gccctttcgg aatgatccgt tctggttttc tcacatatat atacaggaac acccaggtat
     1021 gatccgtacc ttcgcgcatc cctggcgaat cccgcatcgg gggataccac gaccagatcc
     1081 gctgttcccc ttgatttcat atactcacaa agcaggggat aggcatacag gttgtcaaca
     1141 ggcttcttga aaaagccctg aatctgcgga ctgtgaagat ctatcgttat taccctgtca
     1201 gcaccggcaa tttctataca gtcggcacaa acacgggcac gtatcgacac tctcggttca
     1261 tcctttttgt cagccttggc ataactgaaa tatggcatta ttaccgtgac cgatctggca
     1321 ctggaccgtt tgaacgaatc gatccagaaa agaagctcca tgaatgaatc attggctttc
     1381 agcccgactg tctgaacaat ataaacatcc ttgttacgca cgttttcccc gatcttcaca
     1441 tatgtattcc cttcgctgaa ctggaatact tccgatatcc cggcttctat acccaggtag
     1501 ctgcatattt ttccggcgaa ggccctgctt gaagaaccgg caaaaatcct tatccgctcg
     1561 tttctttttt ccactatgct aaatttttat atcaagtttt tatctgaaaa gccagacagc
     1621 atcaaaatta atactttttt caggcttctg attcctggca gatgaaattg gataaagcaa
     1681 aactaatttt actaatacat tgatattcag caatataaaa aatcgctgaa atactgctgg
     1741 ccagcagtcc cggcaattct ttccagttta tccgatcttg ctaatatcct gcaacttatg
     1801 gttgaaaaaa tttggatttt ctcaaactag ttcttatatt tgtgatgcaa tactgttagt
     1861 cctgaacata cgtaactact tactaccggt ttaggtttta ctgttctgtt ttaccatcgt
     1921 actacctgaa tgtgatgtga agtttaaaat gtaaaattat ggaactgaaa gaactaactg
     1981 ttgagaaatt gaccgatcaa tgccgggatc tggacaggaa gtatgagctt ctacgcgctg
     2041 aatacgagca atcaaaggat ctgtactcag caatgccttc ttttgagaat cacgaaaagc
     2101 tgcatgacgt tcacgacagg ctgatccggt gtctggaaga attgagtgat tcccagaaca
     2161 agctgattga agcctattgg tcttattttg gagtaagaca ctggcagttc aaggcgatta
     2221 aggacggaag cggaataatg cgggcatgaa taatccttaa ttctgaaatt ttaaataagt
     2281 gaaacggaaa agaatccgca tccctgcggc tgccggctga tttcctgact gcacaggcct
     2341 gattcactga ttttcttttt tacctgatcc ccaaatcatc ctgtaaagac tatctgcatt
     2401 ctggtgccgg aaagaaaata catgacatat caaatgtttg ataagagtat tttagtttaa
     2461 tttgcggtta aaaccggaat aacttattca gaacaaaata acacacctat ggacaaaaag
     2521 gatcttgaac aaacgctggt acttataaaa ccggacgcac taaaaaattc tctgacagga
     2581 tatattcttt ccgtattatc agaattccac acggggctgc attactgcgg gaccaagatt
     2641 gtgcatgttg gcaggatgct ggctgaggaa cattatgccg aacacagggg gaaggtgttt
     2701 tttcctgcta ttcttgaata cctgatggga gagagtcatt atcctgacaa tccgatgaag
     2761 cgcagggtta ttgctatcgt ataccaggga agggatgcca taaaaaaaat cagggaggtt
     2821 gtaggaccta cgaatcctct taatgccaga gtggaaaaac cgggaagtat ccgttcactc
     2881 ggtacaatgg ttccactgaa ggatgatgac ggcaatgtga tcgggcagcg tatggacaat
     2941 ctcattcacg catctgacac gccacaatcg gcagaaaggg aaattaaact gtggttcagg
     3001 cctgatgaca tgccgccttt gatgcacacc tacccgacgg aggcaagcgg tgaattttat
     3061 tatttcaggg ataacaggct tttcagggaa tatgaaaagg gcagcgtttg tattcttgct
     3121 cctgccgatg ttgcatggaa atcagatctg gaaatattgc acaatataag cagggggctt
     3181 cccgcagccg gatcccttca gtcggttgtt gcaaaatatc tgataaattc cgcaactgaa
     3241 taagcataaa aggctgatca gattttacgg tttattttcc tatacagaag tttctgaaaa
     3301 tattctgaag tatctcgtcg gtggttattt cccccgttat ttcgcctagg taatgaattg
     3361 catggcggat atcggtggca atgaagtctt cctggagatt gttttcaagt ccggtcctta
     3421 ccctttccag gctttcggca acatgaagca gggcctcata atgcctcaca ttggaaatta
     3481 tcacattttc cgtattcagt cttccggcac cggcaagcct tcccagaagc tttttcagtt
     3541 catcaatgcc ttcaccggtt tttgccgata tgaacaacat tgattcatcc tgctttggta
     3601 aaatattctc ctgcatcagc tgcctgtttc cgtataaatc attatccgtc ttgtttacga
     3661 gtatcactat ccttttttca tccgtaagct gcccgcgtat ttttttcagc agggcattaa
     3721 gcggaccggc cccttcgtta atatccgcca gcaggagaat aaccgaggca gctgaaattt
     3781 tttcatgtgt cttttttatt cccaggtttt caattatgtc ggttgtgtcc ctcagaccgg
     3841 ctgtatcaat aaacctgaac cggattccct caatgaccac tgtgtcctcg atggcatccc
     3901 tggtagttcc gggtatatct gacacaattg ccctttcctc catcagcagc ctgttcagca
     3961 gagttgattt gccggtattg ggattgcccg caatcacaac gggaatacca tttttcacgg
     4021 catttccgag actgaaggat gatgacagct tccgggaaac attcagtacc ctgtcaacaa
     4081 tttcgaacag gcttttcctg tcggcaaatt cgacatcctc ctccccgaaa tccagttcca
     4141 gttcaatcag tgaggcaaaa tgaagcaggt cggctctcag aacagctatc tcatcagaga
     4201 atgaaccctt catctgcttc atcgcgatcc tgtgggccat ctttgattca gatgcaacca
     4261 ggtctgcaat ggcttcagcc cgggaaagat ctatcctgcc gtttaaaaag gctcgctggg
     4321 tgaattcccc cggtctggca gcaactgctc cgtggcttat gagcagttcc attatcttct
     4381 gctgtatata gggagaagcg tgacaggata tttctgcaga atcctcaccg gtgaaagaat
     4441 ggggagcacg gaaaatactt atgagcactt catcaattat ttcgtctccc gatttaatat
     4501 cgccaaggac aatggtaaat cccttctgtt ccttcagcct gcgtttcggg tcagccggaa
     4561 caaatatcct ttcacagatt tcaatgctct gcgggccgct tattcttatt atggcaattg
     4621 ccccgcctcc ggatgtggcc ggcgcacaga cagtcgtttc gcttattaac attccgcttc
     4681 tccttaacaa acgcaaagtt aaaaaacatt atccactgtc aggtgcccct tttatctctc
     4741 cagattctga acggtgaaga atgaatcctg tactgtgacc ttgtcattaa tattgctgaa
     4801 caggatctcg gattttcttc ccgtgatctg gttgctggca atcatgttct ttatcatgta
     4861 ttttccatcc tggaacgact ggacgctttt tgtttcaatg attttgaaaa gatcattatc
     4921 gaaattgtag aattccattt tctgcacctg gaacttatcc atgcagacat aggttatttt
     4981 ttttgaataa ccgtattcat atacaatgtc atcatttttc ggaatgcttt cgatcaccca
     5041 tgtattggca gttccggagc tgcctgtatg cctgtgggtg aaatccgaaa gagtggggga
     5101 gctcatatcc gcgtttgaga attcagaact cataaagctt ttaccctttt cccccgatac
     5161 tattcttctt gtttttttaa gtgcgggaag atagatccac atttcatcag ctttatcctt
     5221 gttgtcaata acaagcattg acgtacccct gacatcggct ggttcgacaa agcggatgaa
     5281 tcgtttctcg agaccgtcag gataactctt tgt
//
LOCUS       DAOSPX010000225         5283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14253_length_5283_cov_3.349847, whole genome shotgun sequence.
ACCESSION   DAOSPX010000225 DAOSPX010000000
VERSION     DAOSPX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14253_length_5283_cov_3.349847"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLZ75_10335"
     CDS             complement(<1..387)
                     /locus_tag="PLZ75_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HOS72829.1"
                     /translation="MSKIKVAINGFGRIGRNVFKIALERNDIEIVGINDITDTRTLAH
                     LLKYDSTQGKYPGVSCDDENIIVNGVKVKVTAERNPADIPWSKTPDVVVESTGIFTSK
                     ESPKGGYGDHLKNGAKKVLLTVPSKDD"
     gene            complement(476..1273)
                     /locus_tag="PLZ75_10340"
     CDS             complement(476..1273)
                     /locus_tag="PLZ75_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72830.1"
                     /translation="MRKLLITASFLYSVATVLHGQGELDLQPRVFFRNERSFGVSINS
                     DGLGIGYREGKRKDYRNKKLLEFDMGTLKHPKEYKISNPYTQGSFVFGKLNNVMYIRG
                     GIGHQHEMFSKEDAGGVAIRYFISGGPVIGLYKPIYYRVLYPVGGVLNEFELRDEKFD
                     LTFHDPSMIYSKASFTRGLNETKALPGLYAKGGFNFEYSRQDKVIHAIEIGSQINAFP
                     KKMPIMSSEDNKAIFFSIFVSYRFGVIVDPLHPEDYRITNIFRRKKA"
     gene            1810..2694
                     /locus_tag="PLZ75_10345"
     CDS             1810..2694
                     /locus_tag="PLZ75_10345"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOS72831.1"
                     /translation="MSTESYSQEKLSFNILSKSESAVIDKKGTERPFTGKYNDHRENG
                     TYICKKCGAALYHSSDKFDSGCGWPSFDDEIKGAVNRSRDADGIRTEITCSACGAHLG
                     HVFSGEGLTPKNLRHCVNSLSLDFVPASLESGRYGTAIFAGGCFWGVEYFMQKAPGVV
                     SAVSGYTGGHVKNPSYREVCTGNTGHAEAVRIVYDPEKTSYEKLLRLFLEIHDPTQAG
                     GQGPDIGNQYRSEIFYMNEEQKKIAERNLKALADRGYKIATAVSRADEFYDAEKYHQD
                     YYFLNGKLPYCHSYTKRF"
     gene            2889..4427
                     /locus_tag="PLZ75_10350"
     CDS             2889..4427
                     /locus_tag="PLZ75_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS72832.1"
                     /translation="MIFRKKIQRTSVSRNTKYWFFISGLVVFALLTFSYKKAIDITST
                     DKFCASCHVHPDADRSWKLSTHYNNKSGVVVHCVECHLPPRGQGFFLAKARTGLKDLY
                     GYIFKDTADIDWEMKKLLEYAKKHVYESSCIECHENLYPVTLSVNGGNAHLLYETSKD
                     PVHCLNCHLNVGHYDKHAALHAADSSFGITVTSTGPPFPEAAKVERFEDFTEMIPGTR
                     VSFDMVALPGGKFRMGSGENEPLRNDDEGPVREVTLSPFWIGRTEVTWDEYLAFFRET
                     SSQGRTEGQVVTTGKTDAISGATPPWGAPDQGWGKGARPAITMTWHAASVYCQWLSQV
                     TGKKYRLPTEAEWEYACRGGTETPYFFEGNPKKFTSRGFLKKFIGPDTANIASSVVYM
                     VNSPARTQEPSFVRPNPFGLLNMLGNVAEFCLDYYSADYYAADSVRVNPRGPARGKEH
                     VIRGGSFRSDAKDVRSAARASTRTDAWLVTDPQMPKSIWWYSDCIDVGFRVVCEIDSL
                     KSER"
     gene            4443..>5283
                     /locus_tag="PLZ75_10355"
     CDS             4443..>5283
                     /locus_tag="PLZ75_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72833.1"
                     /translation="MDIKRRDFIGKAALAGFGVAGAGAVLSACRRKKADNKELGLPPI
                     LKGAPRGKKLRAGLVGAGARGTGAAINFISSGPDLEIIALADIFQDKIDACRERFASF
                     NLPIPEENCFAGFDGYKKLMAIPEVDVVLLVTPPRFRPDHFLEAVLTNKHVFMVKPLA
                     VDPVGIRSIITSAKKASAVGLNVVTGTQRRHQEDYIETYRQVASGAIGTITSAKAFWN
                     QDHVWFREREPDWDDMTYMLRNWNNFCWLCGDHILDTHVHNIDVINWFMGKYPERAVG
                     YGGR"
BASE COUNT         1454 a         1126 c         1266 g         1437 t
ORIGIN      
        1 gtcatccttg gaaggaaccg taagaaggac tttctttgct ccgttcttca ggtgatcgcc
       61 atagccgcct ttcgggcttt ctttcgaggt aaagataccg gttgactcaa cgacaacatc
      121 cggtgtcttg ctccatggaa tgtcagcagg attcctttcc gcagttactt ttacctttac
      181 accgtttacg atgatatttt catcatcaca ggaaactccg gggtatttgc cctgggttga
      241 gtcgtatttc aggagatgag ccagcgtcct ggtatcagta atatcgttta taccgacgat
      301 ctcaatatca tttctttcaa gggcaatttt aaatacgttg cgccctattc tgccaaagcc
      361 gtttatggct actttgattt ttgacatatc tctataatta atttggtgaa taattcaaaa
      421 tcgagcacaa aaataataat ttctgaaaaa tcagcaactg atattgaagt tgtgcctaag
      481 ctttttttct ccggaagata tttgtgatcc tgtaatcctc gggatggaga ggatcaacaa
      541 ttacgccgaa tctgtaactc acaaaaattg agaagaatat ggctttattg tcctcactgg
      601 acattatcgg catctttttg ggaaaggcat tgatctggct tcctatttct atggcgtgga
      661 ttaccttgtc ctggcggctg tattcaaaat tgaagcctcc ctttgcataa aggcccggca
      721 gggcttttgt ctcgttgagc cccctggtga aggatgcctt actgtatatc attgagggat
      781 catggaatgt aaggtcgaat ttctcatccc tcagctcaaa ttcattgaga acaccgccga
      841 caggatataa aaccctgtag tatattggtt tgtacaatcc aatcaccggt ccgccggaaa
      901 tgaaatacct gatggccact cccccggcat cttccttgga gaacatttca tgctggtgac
      961 cgattcctcc cctgatatac atcacgttat tcagtttccc gaaaacaaat gatccctggg
     1021 tgtacggatt gctgatcttg tattccttgg gatgcttcag ggtccccata tcaaattcca
     1081 gcagcttctt gttgcggtaa tcctttcttt ttccttcacg gtaacctatt cccagaccat
     1141 ccgaattaat ggataccccg aaggatcttt cattgcggaa aaataccctt ggctgaaggt
     1201 caagctctcc ctgcccgtgg agcactgttg ctacggagta aagaaaagaa gccgtgataa
     1261 gaagctttct cattgtaaat gctgcttaca ggagggaatg atatgaaatg tggcttaaaa
     1321 aatatgtata atatgctata ttatactata ccaagcctgt atctcccggc tgttccgttt
     1381 cagatttgct gtatgcgggg catactttac tgttacatga tcctaaaaca aaaacagtaa
     1441 caacaaataa aaccagtatt acagcaattt tcttcataat gaaaattaat tattaagtac
     1501 aaaagtacga ttttacctta ataaacaata tttgtgtccg gatattgttt cttaaaccgt
     1561 ttattacagc aagttttaaa ttatgctatt gtattgctta caacaaatta atgcaaaaaa
     1621 agttgaattt tcctaatgtt taacgtaaat cttataagtt ttgtttgatt gtgaataaat
     1681 tattgacggt agcaaacggt ttcgggatac tttttcaact ttttgcactg tgaaatgttt
     1741 atattgtcaa attgtgatac tatgataaaa agaattacaa actatttaat tatcagtata
     1801 ttattaatca tgagcactga atcatattca caggagaagc tttctttcaa catactcagc
     1861 aaatctgaat cggcagttat tgacaagaag ggaacggaaa ggccctttac cggaaaatat
     1921 aacgatcacc gggaaaatgg cacttatata tgcaaaaaat gcggagcggc cctgtatcat
     1981 tcttccgata agtttgattc cggatgcgga tggcccagtt ttgacgatga aataaaaggt
     2041 gcggtaaaca gatcccgtga tgcggacggc atcaggacgg aaatcacctg ttcagcctgc
     2101 ggggcacatc tcggacacgt attcagcgga gagggactga ctcctaagaa tctgcggcac
     2161 tgtgtcaatt cgctttccct ggactttgtt ccggccagcc ttgaatccgg aagatacgga
     2221 actgccatat ttgccggggg atgtttctgg ggtgtggagt atttcatgca gaaagctccg
     2281 ggtgtcgtgt ccgcagtatc cggatataca ggcggacatg tgaaaaatcc ttcatacagg
     2341 gaggtatgta ccggaaatac cggtcatgcc gaagctgtca ggattgttta tgaccctgaa
     2401 aagacaagct atgaaaaact tctcaggctt tttctcgaaa tacatgaccc gacgcaggca
     2461 gggggacagg gaccggatat aggcaaccag taccgctcgg aaatattcta catgaacgag
     2521 gaacaaaaga aaattgctga aaggaatctt aaagcactgg ctgacagggg ttataaaata
     2581 gcaaccgcag tcagcagggc agatgaattc tatgatgcgg aaaaatatca ccaggattac
     2641 tatttcctga atggtaaatt accctattgc cacagctata caaaaagatt ctgaaataaa
     2701 tattcctctg acagaaagga aattatcttc cggagtaagg tataaaaggt agctgtatta
     2761 aaggacggtc ctgttccggc catcctcatt cccggcacgg tggttttaaa aaattaacaa
     2821 ttattttttg acgggataat tattaattca attaacttta gcgcttttaa ttgatttctt
     2881 cccgcaaaat gatctttcgc aagaaaatac agaggacttc tgtttcccgc aacactaaat
     2941 actggttttt tatttctggc ctggttgttt tcgccctgct gacattttcc tataaaaagg
     3001 caatagacat tacatcaacc gacaagttct gtgcgtcatg ccatgtacat ccggatgcgg
     3061 accggagctg gaagctctcg acacactaca acaacaaatc aggcgttgtt gtgcattgtg
     3121 ttgagtgtca tcttccgccc cgtggtcagg gttttttcct ggcaaaggcc aggacgggcc
     3181 tgaaggatct ttacggctat attttcaagg atacggcgga tatcgactgg gagatgaaga
     3241 agcttctgga gtatgccaag aagcatgttt atgagagctc ctgcattgaa tgtcatgaaa
     3301 acctttaccc ggtcacactt tccgtaaacg gggggaatgc ccatcttctt tatgaaacaa
     3361 gcaaggatcc cgtccattgc ctgaactgtc atctcaatgt cggtcattat gataagcatg
     3421 cggcacttca tgctgctgat tcgtcctttg gcattacagt tacctcaacc gggcctccgt
     3481 ttccggaagc agcaaaggtt gagcggttcg aagactttac tgaaatgatt cccggcacaa
     3541 gggtgtcatt cgacatggtg gcgcttcccg gaggaaaatt ccgaatggga tccggtgaga
     3601 atgagccatt gcgcaatgat gatgagggac cggtaaggga agtaacactt tcgccgttct
     3661 ggataggcag aacggaggtt acctgggatg aatacctggc atttttcagg gaaacgagtt
     3721 ctcagggaag gaccgaaggc caggttgtga ctacgggaaa aaccgatgca atcagcggtg
     3781 ccactcctcc ctggggagca cccgatcagg gctgggggaa gggagcaaga ccggcaataa
     3841 ccatgacgtg gcatgcagcc agtgtatatt gccagtggct gtcgcaggta acaggaaaaa
     3901 aatacaggct gcccacggaa gctgaatggg aatacgcctg caggggcgga acagaaacac
     3961 cttatttttt tgagggtaat ccgaaaaaat tcacttcccg gggtttcctg aaaaaattca
     4021 tcggtccgga tacagccaat attgcttcat cagtggtcta tatggtgaac agccctgcca
     4081 ggacacagga accgtccttt gtcagaccca atcccttcgg tctgcttaac atgcttggga
     4141 atgttgcgga attctgcctg gattattact ctgccgacta ttatgcagct gattccgtga
     4201 gggtgaatcc gcgaggtccg gccaggggaa aagagcatgt aatcagaggc ggatcgttcc
     4261 gaagcgatgc aaaggatgtg cggagtgccg cacgtgcatc cacgcggaca gatgcatggc
     4321 ttgtgaccga tccccagatg cccaaaagca tatggtggta ttccgattgc attgacgtgg
     4381 gattcagggt ggtgtgtgag attgactcat taaagtctga acgttaaaat ttcatttcag
     4441 ctatggatat aaaaagaaga gatttcatag ggaaagcagc acttgccgga tttggcgttg
     4501 ccggggccgg cgcggtcctg tcggcctgca ggagaaagaa agcggataat aaggaactgg
     4561 gacttccgcc gatattgaaa ggtgcgccca ggggaaagaa actgagggcc gggcttgtgg
     4621 gggcgggagc aaggggaaca ggtgcagcca ttaatttcat cagttcgggt cctgatcttg
     4681 agataattgc actggcagat atttttcagg acaagatcga tgcctgccgg gaaagatttg
     4741 cttcattcaa tctgcccatt cctgaggaaa actgttttgc cggttttgac ggatataaaa
     4801 aactgatggc gatacccgag gtggatgttg tgcttctcgt aacgcctccc cgcttccggc
     4861 ccgatcattt tctcgaagct gtccttacaa acaagcatgt tttcatggtg aaacccctgg
     4921 cagttgatcc tgtaggtatc agatccatta tcacttcggc caaaaaggcc agcgctgtgg
     4981 gtctgaatgt tgttacaggc acccagcgca ggcatcagga ggattatatt gaaacctaca
     5041 ggcaggttgc atcaggtgca atcggtacca ttacttcggc aaaggctttc tggaaccagg
     5101 accatgtatg gttccgtgag cgtgaaccgg actgggatga catgacctac atgctgagga
     5161 actggaataa tttctgctgg ctctgcgggg atcatatcct tgatacgcat gttcacaata
     5221 ttgacgtgat caactggttt atggggaaat acccggaaag ggccgtagga tacggaggaa
     5281 ggg
//
LOCUS       DAOSPX010000226         5275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14276_length_5275_cov_4.262261, whole genome shotgun sequence.
ACCESSION   DAOSPX010000226 DAOSPX010000000
VERSION     DAOSPX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14276_length_5275_cov_4.262261"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLZ75_10360"
     CDS             <1..498
                     /locus_tag="PLZ75_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOS72834.1"
                     /translation="GDAELLLKAGADRISVNSSAVRNPGLINELADRFGSRCIVVAID
                     TNRIGDDWIVFTGGGRVSSGLKTVEWAVEAARRGAGEILLTSMMNDGTRSGYSIDITA
                     DVSRNVDIPVIASGGAGKKEHFKSVFLNTDCSAALAASIFHYGEIKIRKLKEYLYREH
                     IPVRL"
     gene            526..657
                     /locus_tag="PLZ75_10365"
     CDS             526..657
                     /locus_tag="PLZ75_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72835.1"
                     /translation="MKDPEKQQEYFPAGGKPYGIRKKGNPENNRKNLLTSGKDGKSF"
     gene            657..1307
                     /gene="hisIE"
                     /locus_tag="PLZ75_10370"
     CDS             657..1307
                     /gene="hisIE"
                     /locus_tag="PLZ75_10370"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOS72836.1"
                     /translation="MSVKDREEQKRTYRRLNMKNMDFEKHMGLIPVVIQDNTTLQVLM
                     VGFMNEEALARTLNEGKVTFFSRSKKRLWTKGETSGNFLYVKEMHPDCDSDSLLIRVD
                     PAGPVCHTGNTSCFSNDSSRGFLYRLEKIVGDRIDEDAKDSYTNRIFREGINKAAQKV
                     GEEAVELIIEAKDNNNELFINEAADLLYHLLILFRAKGISVRDVEDCLASRHSASR"
     gene            1600..2430
                     /locus_tag="PLZ75_10375"
     CDS             1600..2430
                     /locus_tag="PLZ75_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOS72837.1"
                     /translation="MKTAIQGEKGSFHEVAARQYFRNESIEIVPCTTFDLTLIAVREG
                     LADFAMIAIENARSGSILSNYTLIRESGMKILGEHNLRIVQNLMALPGQSLETIREIR
                     SHPIAFNQCMSYLNHLKNVTLIEADDTAGSARYISENRLMGVAAISSAISAQIYGLEI
                     IAPGIETYKKNYTRFLLVGEKDKAGREGNKISVSFCTGHRPGSLAAVLVRLAELDINL
                     SKIQSVPRLNGDWEYMFYADLELNPDHDINQIKNVLQEYATELEILGIYNKGDKLYES
                     "
     gene            2369..3580
                     /locus_tag="PLZ75_10380"
     CDS             2369..3580
                     /locus_tag="PLZ75_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS72838.1"
                     /translation="MPRNWKSWESITREINYMRVNPAIRTESVREYYFSQKLRQIDQM
                     RKEGADIINLGIGSPDQPPSQNTIARLAGEAMKPSSHGYQSYNGIPALRKAFADWYRK
                     YFGIELDPDSEILPLMGSKEGIMHISMAFVNPGDEVLVPNPGYPTYSSVTNLTGGKVR
                     YYDLTGENGWHPDFERLEDSDLEKVKLMWINYPHMPTGAKGSARLFDRLTEFGLKHGI
                     LLCNDNPYSFILNNDYLSLLGADGAKEIALELNSLSKSHNMAGWRIGLVAGHRDYINT
                     ILKVKSNMDSGMFRALQEAAAEALGNPDSWYEGLNSVYSSRRLIVEELMNILNCRFDP
                     EQTGMFVWGRIPDNTESCEKFVDDILHRHHVFITPGFIFGSGGDRYIRISLCADEKTL
                     NEAKRRLTSEQ"
     gene            3577..4422
                     /locus_tag="PLZ75_10385"
     CDS             3577..4422
                     /locus_tag="PLZ75_10385"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOS72839.1"
                     /translation="MTIAVIGTGLIGGSLILSLRKREFADRIIGVENNIQHRNIALLS
                     GMIDEADTLENAVDKSDLIILCAPVDVNCALLPRILDRIEGTSKIVTDMGSTKADMAE
                     AAKNHPARGRYVAVHPMAGTEYSGPLAAIGRLFDYKIVIICDAGLSDPDALETTERML
                     TSLNMRITYMDSAMHDVHVAYISHISHITSFSLALCVLEKEQEEENIMTLAGGGFEST
                     VRLAKSNGETWAPIFTENAEYILEVIDTYIEKMNLFRSLISRRDKEGLKQLMEEANKI
                     QKILN"
     gene            4634..>5275
                     /locus_tag="PLZ75_10390"
     CDS             4634..>5275
                     /locus_tag="PLZ75_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOS72840.1"
                     /translation="MVIKLETIPVTEWKGYSKRPFLISGPCSAETEEQVMETARQLAK
                     IEQVSLFRAGIWKPRTRPDSFEGIGTPALKWLRNVKEETGLLVGCEVANERHVYETLK
                     YGIDMLWIGARTSVNPFTVQEIANALNGVDVMVMIKNPINPDIDLWIGAIERIARAGI
                     KRIGAIHRGFSSYEKTSYRNQPNWQLPIELRRKMPDIPIICDPSHIAGARQYLH"
BASE COUNT         1635 a         1153 c         1327 g         1160 t
ORIGIN      
        1 ggggatgctg agcttcttct gaaagccggg gctgacagga tatcggtgaa ttcctctgcc
       61 gtaaggaatc ccggactgat aaacgaactg gctgacaggt tcggaagccg gtgcattgtc
      121 gtggccatag acacaaacag gattggagat gactggatag tgtttaccgg cggaggacgg
      181 gtgtcgtcag ggctgaaaac ggtggaatgg gccgtggagg cagccagacg gggagccggc
      241 gagatactgc tcacatcaat gatgaacgac ggtaccaggt cgggatactc cattgatata
      301 acggcagatg tgagcagaaa tgttgacatc ccggtaatag cttccggagg agcaggtaaa
      361 aaggaacatt tcaaaagcgt tttcctcaat accgattgca gcgctgcgct ggcagcaagc
      421 attttccatt acggggaaat taagatcagg aaactcaagg aatatctcta cagggaacat
      481 ataccggtaa gattgtaata atatatctgg ctcgtttcag gggatatgaa ggatcctgaa
      541 aaacaacagg aatactttcc ggcaggcgga aaaccttacg gtatccggaa aaaaggaaat
      601 ccggaaaaca accggaaaaa ccttctgaca tcaggaaaag acggcaagtc cttctgatat
      661 ccgtaaaaga cagggaagag caaaagagaa cttataggag attaaatatg aaaaacatgg
      721 attttgaaaa gcatatgggc ctgattcctg tcgtgataca ggacaatacc acacttcagg
      781 tactgatggt aggattcatg aatgaggaag ctctggccag aaccctgaat gaaggaaaag
      841 ttactttctt cagcaggagc aaaaagcgtt tgtggacaaa gggagagaca tcaggcaatt
      901 ttctgtatgt aaaagagatg catcccgact gcgacagtga ttccctgctg ataagggttg
      961 accccgccgg acctgtttgc cacacgggaa acacatcctg cttcagcaat gattcttcca
     1021 ggggattcct gtacaggctg gaaaaaattg tcggggacag aatcgatgaa gacgcaaagg
     1081 attcatatac aaaccgcata ttcagggagg gaatcaacaa ggctgcacag aaggtcgggg
     1141 aagaagcggt tgaactgatc attgaagcca aggataacaa caatgaactg tttatcaatg
     1201 aagccgccga cctcctgtac catcttctga tacttttcag ggcaaaggga atttcggtcc
     1261 gggatgtgga agactgcctc gcttcacggc attccgcttc ccgctgaatt actcctcctg
     1321 caatccggcc tttaccggga tccagcgtta tatgggacct ttccccgtgc cggacctttt
     1381 ttaatttttc ttgctttttt cttgtaattg taaaaaatag tttaattttg tcagcagaaa
     1441 tgcgacttga tgaataatac acaaaactat aacacctcct attttttctt cttttacttt
     1501 ttgaaagaat aaggggattt ttgtgtaaaa atgaaataaa taccaatgaa gcaaagtccc
     1561 cggaaaccgg ggactttttt ttataaatat gcataagaca tgaaaacagc aatccaggga
     1621 gaaaagggat ccttccacga agtggcagcc agacagtatt tcagaaacga aagcattgaa
     1681 atagtgccct gcacgacatt tgacctcaca cttattgcag tcagggaagg actggcggat
     1741 tttgcaatga ttgccataga aaatgcgagg tccggaagca ttttgtcaaa ctataccctg
     1801 atacgtgaat cgggcatgaa aatactcggg gaacacaatc tgagaatagt gcagaacctg
     1861 atggcactgc cgggccagtc tctcgaaaca atcagggaaa tacgctcaca tccaattgcc
     1921 tttaaccagt gcatgtcata tctcaaccat ttgaaaaatg ttaccctgat tgaagctgac
     1981 gacactgcgg gaagcgccag gtatatcagt gaaaacagac tgatgggggt tgctgctatc
     2041 tcttcagcaa tatctgcaca gatatatggc ctggaaatca tcgctcccgg aatcgagacc
     2101 tataaaaaga attacaccag attcctgctg gtaggcgaaa aagacaaggc aggcagagaa
     2161 gggaacaaga tttccgtaag cttctgtacg ggccacaggc cgggatcact ggcagccgta
     2221 ctggtccgcc ttgcggaact tgacatcaac ctgtcaaaga tacagtcggt gccccggctg
     2281 aatggcgact gggagtatat gttctatgcg gatcttgaac tgaatccgga tcatgatatc
     2341 aaccagataa aaaatgttct gcaggaatat gccacggaac tggaaatcct gggaatctat
     2401 aacaagggag ataaattata tgagagttaa tccggcaata cgtacagaga gcgtcaggga
     2461 gtattatttc tcgcagaagc tgaggcagat agaccagatg cggaaagagg gcgcagatat
     2521 cattaacctt ggtatcggaa gccctgatca gcctccatcg caaaacacaa ttgcccgact
     2581 tgccggggag gccatgaagc cttcctcaca cggttaccaa agctacaacg gcatacctgc
     2641 cctcaggaaa gcttttgccg actggtacag gaaatatttc ggaattgaac ttgatccgga
     2701 ttcggaaatc cttccgctga tgggcagcaa ggagggtata atgcatataa gcatggcatt
     2761 cgtgaatccc ggcgatgagg tcctggtgcc aaacccggga tatcccacct attcatccgt
     2821 aacaaatctt accgggggca aggttagata ctatgacctt accggagaga atggatggca
     2881 tcccgatttt gaaaggctcg aggacagtga tcttgaaaag gtaaagctca tgtggatcaa
     2941 ttatcctcac atgcccacgg gtgcgaaagg ttcagccagg ctttttgaca ggctgacgga
     3001 gttcggatta aaacacggta ttcttctctg caatgacaat ccctacagct tcatcctgaa
     3061 caatgattac ctgagcctgc tcggagctga cggggcaaag gagattgccc ttgaactcaa
     3121 ttcgctcagc aaatcccata acatggccgg atggaggata ggactcgtgg cgggtcaccg
     3181 tgattatata aacaccatcc ttaaggttaa aagcaacatg gattcgggaa tgttccgtgc
     3241 actgcaggaa gctgccgccg aagcactggg aaaccccgat tcatggtacg aaggcctaaa
     3301 cagtgtctat agctccaggc ggctgatcgt tgaagaatta atgaatatcc tgaattgcag
     3361 attcgacccg gagcagacag gcatgtttgt atggggacgc attcctgaca acacggagag
     3421 ctgtgaaaaa ttcgtggatg acatccttca caggcaccat gtattcatta ctccgggatt
     3481 cattttcgga tcaggaggcg acagatacat acgcatatcg ctgtgcgcgg atgaaaaaac
     3541 actgaatgaa gcaaaaagaa gattaacatc tgaacaatga caattgcagt aataggtacg
     3601 ggtctaatcg gaggatcact gatcctcagc ctgcggaaaa gagaatttgc cgacaggata
     3661 ataggagttg aaaacaacat ccagcacagg aatattgccc tgctcagcgg tatgatagat
     3721 gaagccgata ccctggagaa tgcagtggac aaaagcgatc tgataatcct ttgcgcaccg
     3781 gttgacgtta attgtgccct tctgccacgg atacttgacc gcattgaagg cacttcaaaa
     3841 atagtgaccg acatgggttc aaccaaggca gatatggccg aagcagcgaa aaaccatccg
     3901 gcaaggggaa gatatgtggc cgtccatccg atggcaggaa cggaatattc tggtccgctg
     3961 gcagccatcg gaaggctgtt tgactataaa atagtgatta tctgcgacgc cgggctgagc
     4021 gatcccgatg cgctggaaac cacggaaaga atgctgacaa gcctcaatat gcggatcacc
     4081 tacatggatt cagcaatgca tgacgttcat gtggcataca tatcccatat ttcccatata
     4141 acctcctttt cactggccct gtgcgtgctt gaaaaggaac aggaggaaga aaacataatg
     4201 acactcgccg ggggaggatt tgaaagtacc gtccgccttg caaaaagcaa cggtgaaacc
     4261 tgggctccga tctttacgga aaacgcagaa tatattcttg aagtaataga cacatacatt
     4321 gaaaaaatga atcttttcag atccctcatc tcccgcaggg ataaggaagg actgaaacag
     4381 cttatggagg aagcaaataa aatacagaaa atcctgaatt agcaggccgg atgaaagcct
     4441 gcctgataat ccgcggaatc atcaggatta gaggggaaca gtgcagtccg gacagatcag
     4501 gataaatgaa attcagacag aacggaaaga tcaggataaa tgaaattcag acagtgcgac
     4561 agatcaggac aaatgaattc agacagacga acagataaga ataataaatt tcagtcactt
     4621 atattttaaa accatggtaa tcaaactgga aacaattccc gtcacagaat ggaaaggcta
     4681 cagcaaaagg cccttcctca tatcgggtcc ctgcagcgcg gagaccgagg aacaggttat
     4741 ggaaacagcc aggcagcttg caaaaatcga acaggtaagt cttttcaggg caggtatatg
     4801 gaaaccccgg accaggcccg attcatttga gggtataggt accccggcac tgaaatggct
     4861 caggaacgta aaggaggaaa caggactgct tgtcggctgt gaggtggcaa acgagaggca
     4921 tgtgtatgaa actctcaagt atggtataga catgctgtgg ataggagccc gtacatccgt
     4981 caatcccttt accgtacagg aaatagccaa tgccctgaac ggagttgacg tgatggtaat
     5041 gatcaagaac cccatcaacc cggacataga cctgtggata ggtgcaatcg aaaggatagc
     5101 cagggcagga ataaaacgga taggtgccat acaccgcgga ttttcatcct acgaaaaaac
     5161 atcttacagg aatcagccca actggcagct gcccattgaa ctgagaagaa aaatgccgga
     5221 cattccgata atctgtgatc ccagccatat tgccggagcc cggcagtacc tgcat
//
LOCUS       DAOSPX010000227         5241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14409_length_5241_cov_3.605669, whole genome shotgun sequence.
ACCESSION   DAOSPX010000227 DAOSPX010000000
VERSION     DAOSPX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14409_length_5241_cov_3.605669"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLZ75_10395"
     CDS             complement(<1..836)
                     /locus_tag="PLZ75_10395"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOS72841.1"
                     /translation="MPDGNAEYDILIITGPTASGKTATAALVADRLGGEIISADSRQV
                     YRGMDLGTGKDYDDYIVGGKRIPFHLIDIAEAGSKYNLFEYRKDFLRVYNDLKSRNVF
                     PVLCGGSGMYIDSIVSSYSLPEVPPDPALRAELVKKPMADLVRILSSYKRLHNITDID
                     TRKRAVRAIEIETYREAAAFNELSFPRLKYLIAAISTDRESRRRRITARLRQRLESGM
                     IDEVRDLLAGGIDEEDLIYYGLEYKYITLYLSGDIDYETMFRKLETAIHQFAKRQMTW
                     FRG"
     gene            914..1372
                     /gene="smpB"
                     /locus_tag="PLZ75_10400"
     CDS             914..1372
                     /gene="smpB"
                     /locus_tag="PLZ75_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOS72842.1"
                     /translation="MKRQSGNIVIKNRKASHDYEFLEKFTAGMVLTGTEIKSIRLGKA
                     ALVDSYCFFSNSELFIRGMHIAEYYWGNLNNHDPVRDRKLLLTKRELRRIERKVKESG
                     LTIIATKVFITGKGLAKAEIAIARGKKEYDKRETIKRRDSERELDRMMKK"
     gene            complement(1382..2101)
                     /locus_tag="PLZ75_10405"
     CDS             complement(1382..2101)
                     /locus_tag="PLZ75_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOS72843.1"
                     /translation="MGKNDKPADLIRDAFTEKTWNEIHTTDSWRVFKIISELVEGFEK
                     LARIGPCVSIFGSARTHHNDKYYRMAEEIAFKLTQNGYGVITGGGPGIMEAANKGAKR
                     GNGKSVGINIDLPFEQKPNAYIDADKLITFDHFFVRKVMFMKYAQGFIVLPGGFGTFD
                     ELFEALTLIQTRKIGKFPIILVGAKYWKGLVDWIENVMLQEESNIFPEDLSLFTVVDT
                     ADEAVDTISKFYSRYLLSPNF"
     gene            complement(2137..4953)
                     /gene="uvrA"
                     /locus_tag="PLZ75_10410"
     CDS             complement(2137..4953)
                     /gene="uvrA"
                     /locus_tag="PLZ75_10410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOS72844.1"
                     /translation="MGDEIRVLGARVHNLKDIDVTIPRNKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYMETLSVYARQFLGGMERPDVDKISGLSPVISIEQKSTNKNPRSTVGTI
                     TEIYDFLRLLYARAADAYSWVSGEKMVRYTDEQITDLLKERFAGEKVYLLAPVVKGRK
                     GHYRELFEQIRRKGYLSVRVNDEIREITYGMKLDRYKVHFIEIVIDKFMIGEVTDKRL
                     RDSVQTALDQGEGVMMVLGSSSSEPRYFSRHLMDPVSGLSYNEPAPHTFSFNSPQGAC
                     PHCNGLGEISSIDEDKLIPDRSLSIRKGGIRPLGKYRNVWIFWQIEAIAEKNGFTLDT
                     PIGDIPEDALRKILYGSDEVLRLSNTPLGMTSQYTLTFEGVVNFIGNLEAVDEKRNGA
                     RLRDQYVQYETCPECNGSRLKKEALYFRIAGRNIGELAAMDIRELSDFLLHLDEKLTK
                     RQKRIASEILKEIRARVGFLVDVGLDYLSLNRGARTLSGGESQRIRLATQIGSKLVNV
                     LYILDEPSIGLHQRDNRRLIASLRQLRDAGNSVIVVEHDRETILSADHVIDMGPGAGV
                     HGGIVIAEGSPGEIARFNSLTAGFLSNRKRLEIPGSRRTGNGRSLVIRGASGHNLNNL
                     DVRIPLGVLVCVTGVSGSGKSSLINQTLHPVLAGKYHHSGKRSLPYREISGTEYLDKV
                     IEVNQSPIGRTPRSNPATYTGVFTDIRKLFEQTTEARIRGFSAGRFSFNVSGGRCEGC
                     QGAGVKTIEMNFLPDVYVPCVDCNGKRYNRETLEVRYKGKSIGDVLDMTVNMAVEFFA
                     NVPSIYNKIKTLQDVGLGYITLGQQSTTLSGGEAQRVKLSAELSRRDTGKTLYILDEP
                     TTGLHFEDVRVLLDVLNRLVDKGNTVIIIEHNMDVIKMADYIIDLGREGGREGGNLVF
                     SGPPEELVKCDDSYTGRYLKEELE"
     gene            complement(4955..>5241)
                     /locus_tag="PLZ75_10415"
     CDS             complement(4955..>5241)
                     /locus_tag="PLZ75_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOS72845.1"
                     /translation="IVDPSAYGFDIEEDELYPELKYHEVEVKGEIADFSDFAASYGTN
                     YKLLKFLNPWLRQPYLNPRQGKTYLIKIPAEGMRNPESIESFSRIYYDSQ"
BASE COUNT         1332 a         1409 c         1124 g         1376 t
ORIGIN      
        1 ccccggaacc aggtcatctg cctctttgca aactgatgaa ttgcagtctc cagcttcctg
       61 aacatggtct catagtcaat atccccactc aggtataaag taatgtactt gtattcaagt
      121 ccgtaataaa taaggtcctc ctcatcaata cccccggcca ggagatccct tacctcatct
      181 atcatacccg attcgagacg ctgccggagc ctggccgtta tccttctcct acggctttcc
      241 ctgtcggtgg aaatggctgc aataaggtat ttcaggcggg ggaaggacag ctcattaaaa
      301 gcagcagcct cccggtacgt ctctatctct attgccctta cagcacgctt cctggtatcg
      361 atgtcggtaa tattgtgcag ccgtttgtat gatgacagga ttctgacaag gtcagccatc
      421 ggtttcttta caagttcagc cctgagagcg ggatcgggag gcacttcagg cagactgtag
      481 gacgaaacga tgctgtcgat atacattccc gaacctccgc ataacaccgg gaatacattt
      541 ctgctcttca gatcattgta aaccctcagg aaatccttcc ggtattcaaa taaattgtac
      601 ttcgaccctg cctcagctat gtcgatgaga tgaaacggga tgcgcttccc ccccacaata
      661 tagtcatcat agtccttgcc cgttcccaga tccattcccc ggtaaacctg cctcgaatcg
      721 gcactgatga tttccccgcc caggcggtcc gccacaaggg cggcagttgc agttttcccc
      781 gatgcagtcg gtccggtgat gatcaaaata tcatattccg catttccatc cggcattggg
      841 aaaaagagat tttttttgga acaggcaatt tactaaaaaa agagtatttt tgaacctccc
      901 aggaatcaga aagatgaaaa ggcagtcagg aaatattgtg atcaaaaaca gaaaagcttc
      961 ccatgattat gagtttcttg agaaattcac ggcaggaatg gtccttacgg gaacggaaat
     1021 aaaatcaata agactgggaa aggccgcact ggtggattca tattgctttt tcagcaacag
     1081 cgaattgttc atcaggggaa tgcatatagc cgaatattac tggggaaatc tgaataacca
     1141 cgatcccgtg agggacagaa aactgctgct gacaaaaagg gaactcagaa ggattgaaag
     1201 aaaggtaaag gaatccggcc ttaccattat agcaacaaaa gtgtttatta caggaaaagg
     1261 acttgccaag gccgaaattg ccattgccag gggaaagaag gaatatgaca agagggagac
     1321 catcaaacgc agagacagcg aaagggaact ggacaggatg atgaaaaaat aatcccggct
     1381 atcagaagtt ggggctcagc aggtatcttg aatagaattt cgaaatggtg tcaaccgcct
     1441 cgtcagcagt atccacaaca gtgaacagac tgagatcctc ggggaaaata ttgctttcct
     1501 cctgaagcat tacgttttct atccagtcaa ccagcccctt ccagtatttt gcccctacca
     1561 gaataatcgg aaacttgcct atcttgcgag tctggataag tgtcagggct tcgaaaagct
     1621 catcaaaggt gccaaagcct cccggaagga ctataaatcc ctgggcatac ttcatgaaca
     1681 tcaccttccg cacaaaaaaa tgatcaaagg tgatcagctt gtcagcatca atataggcat
     1741 tcggtttttg ttcaaacgga agatctatgt ttatccctac cgatttcccg tttccgcgtt
     1801 ttgctccctt gtttgcggct tccattattc ccggacctcc tccggtgatc acgccgtaac
     1861 cgttctgtgt gagcttgaat gcaatctcct cggccatccg gtagtatttg tcattgtgat
     1921 gagttcttgc ggatccgaag atggaaacac agggcccgat ccttgccagc ttttcaaatc
     1981 cttctaccag ctctgaaatt attttgaata ccctccacga atcagtagtg tgaatctcgt
     2041 tccatgtctt ctccgtaaaa gcatccctga tcagatcagc cggtttatcg ttttttccca
     2101 tactattcta tttaaaggat tccaaatata taaaaattat tcaagctcct cctttagata
     2161 tcttcccgtg tagctgtcat cacatttgac cagttcctcc ggcggacccg aaaaaaccag
     2221 attaccccct tccctgcctc cttccctgcc cagatcaata atgtaatcag ccatcttgat
     2281 cacatccatg ttatgttcaa taataataac cgtattcccc ttgtcaacga gcctgttgag
     2341 aacatccagc aaaaccctca catcttcaaa atgcaggccg gtggtcggtt cgtcaaggat
     2401 atacagcgtc tttcctgtat ccctccttga gagttcggct gaaagtttca ccctctgggc
     2461 ctccccgccg gaaagagtgg tggactgctg accgagggtg atatatccga gaccgacatc
     2521 ctgaagtgtt tttatcttat tgtaaatcga aggaacattg gcaaaaaact ccaccgccat
     2581 gttaacagtc atatccagga catcccctat ggatttcccc ttatacctga cctccagggt
     2641 ctcccggttg tatctcttgc cgttgcagtc aacacaggga acatatacat cgggcagaaa
     2701 attcatttca atcgttttca caccggcacc ctgacagcct tcacatcttc ccccgcttac
     2761 attgaaggaa aaacgtccgg cactgaagcc ccttatcctg gcttcggtag tctgctcaaa
     2821 cagcttgcgg atgtcggtga aaaccccggt ataggtggca ggattggatc ttggagtccg
     2881 tccgatggga gactggttta cttcaataac cttgtccagg tattcagttc cgcttatctc
     2941 ccggtagggc agtgatcttt tcccggagtg atggtatttt ccggccagaa caggatgaag
     3001 agtctgattt atgagggatg atttgccgct gcctgaaaca ccggttacac agacaaggac
     3061 tccaagggga atccgcacat ccagattgtt caggttatgg cccgaagctc ccctgattac
     3121 aagcgacctg ccattgcctg tccgcctcga tccgggtatt tccagtcttt tccggttgct
     3181 caggaaacct gctgtcagtg aattgaaccg tgcaatctca ccgggacttc cttcggcaat
     3241 gactatcccc ccgtggacgc cggcccccgg acccatgtca atcacatgat ctgccgacag
     3301 gatggtttcc cggtcatgtt caacaactat cacggaattg ccggcatccc tcagctgcct
     3361 gagcgaagct atcagccgcc tgttatccct ctgatgaagc cctatgctgg gctcatccag
     3421 aatatacagc acattcacga gctttgagcc tatctgggta gccagcctta ttctctggct
     3481 ctcgccgccc gacagtgttc tggctccgcg gttcagcgac agataatcaa gacccacatc
     3541 aacaagaaaa ccaacccttg cccttatttc cttcagtatt tcagatgcta tccgcttctg
     3601 gcgctttgtc agcttctcat ccagatgaag aagaaaatcc gaaagctcac gtatgtccat
     3661 ggccgccagt tcaccgatat tcctgccggc aatcctgaaa tacagggcct cttttttcag
     3721 tctgctgcca ttgcactccg gacaggtctc gtactgcaca tactgatccc tgagccttgc
     3781 cccgttacgc ttttcatcaa cagcttcaag attcccgata aaattgacaa cgccttcaaa
     3841 agtcagcgta tattgtgaag tcattccgag aggagtattc gaaagcctca gtacctcatc
     3901 cgatccgtaa agtatcttcc tgagggcatc ctccggaata tcccctatgg gagtgtcaag
     3961 tgtaaacccg ttcttttctg caattgcctc aatctgccag aatatccata cattcctgta
     4021 tttgccgaga ggtctgatcc ctcccttcct tatacttaaa cttctgtcgg gaatcagttt
     4081 gtcttcatct atgctcgata tttcacccag accattgcaa tgaggacagg ccccctgggg
     4141 ggagttgaag gaaaaggtat ggggcgccgg ttcattgtag gaaaggccgc ttacgggatc
     4201 catcagatga cggctgaaat accgcggttc actgctgctg ctgccaagta ccatcatcac
     4261 cccctctccc tggtcaaggg cagtctgaac ggaatcccga agacgtttgt ccgtaacctc
     4321 tccgatcatg aacttgtcaa tcactatctc aataaaatga accttgtagc ggtccagctt
     4381 catcccgtaa gtgatctccc ttatctcatc gtttacgcgt acactcagat aaccctttct
     4441 ccttatctgc tcaaaaagct cccggtaatg gcccttgcgc cccttaacga caggtgccag
     4501 cagatatacc ttttcacctg caaatctctc cttaagcaga tctgtgatct gttcatcggt
     4561 gtatctgacc atcttttcac ccgataccca ggaatatgca tccgctgccc tggcatacag
     4621 gagcctcaga aaatcatata tctcggtaat ggtgcctact gtcgatctcg gattcttgtt
     4681 ggtgctcttc tgctcaattg aaatgacagg actcaggccg cttatcttgt caacgtcggg
     4741 cctttccatg ccgcccagaa actgccgggc atataccgac agggtttcca tgtagcggcg
     4801 ctggccctcg gcatagatag tatcaaaggc aagagacgac ttgccgctgc cgctcagacc
     4861 ggttatcaca accagcttgt tccgcggtat ggtgacatct atatccttca ggttatgaac
     4921 cctggctcca agtactctga tctcgtctcc catcttactg actgtcgtaa tatattctgc
     4981 tgaaggattc aatactttca ggatttctca tgccctcggc cggaatcttg ataagatagg
     5041 tcttcccctg tcgcgggttg agatagggct gccttaacca gggattgagg aatttgagaa
     5101 gcttgtaatt cgttccgtaa ctggcagcaa aatccgaaaa atcagctatt tcacccttaa
     5161 cctccacctc atggtactta agttccggat aaagctcatc ctcctcaata tcaaaaccgt
     5221 aagcagacgg gtcgacaatg a
//
LOCUS       DAOSPX010000228         5222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14472_length_5222_cov_3.915038, whole genome shotgun sequence.
ACCESSION   DAOSPX010000228 DAOSPX010000000
VERSION     DAOSPX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14472_length_5222_cov_3.915038"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLZ75_10420"
     CDS             complement(<1..326)
                     /locus_tag="PLZ75_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72846.1"
                     /translation="MFKISLCVLLFSSFLATAAAAETDTLFMSEEVIRMELRADFTEI
                     ESDRTGTVKPHEGQLTYFNTDGSEVKLPVVVSVRGHFRKDPANCTFPPLMISFREDDV
                     VNTVFRG"
     gene            431..1567
                     /locus_tag="PLZ75_10425"
     CDS             431..1567
                     /locus_tag="PLZ75_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS72847.1"
                     /translation="MKNILCSKYKIRTRCFLISLSLAIASSNHLAASGIECTEGDTVS
                     INDGPYIYFDYDILKVRWIENNNLVEEDLTASGFEDIKEKFNLLYNFHDLQNTFLISP
                     AFRQNYRNIDSIALISDVHGQYDIYINLLKKNGIIGEDLCWKFGKGHLVVLGDVFDRG
                     DKVTEIFWHLFGLEKQALKSGGRVHFLLGNHEAMILGCATSYIHDKYKKVEKITGKYY
                     CDLYSGNSVLGRWLRSKPVIASINDIVFVHAGLSADIIKQKLKIAKINRLFCEKIIAK
                     DYSLIEKNQDLLLLSDENGPLWYRGYFFDRSFNENRIDSVLDFYEKKHVVVGHTVTKE
                     IVSVFNKKIIGCDAGIMYRNPGEMLIVKKGVFHRGLSNGERIKL"
     gene            1609..2982
                     /locus_tag="PLZ75_10430"
     CDS             1609..2982
                     /locus_tag="PLZ75_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72848.1"
                     /translation="MADQNYSCTRRKFIQTAAVAAVGAPFIIRCSPGGRSPNGRLNHA
                     CIGVGGMGWHDLHKFMEDPNVQIVAICDVDTDYLQKASEVLPDARKYTDWRELLDREA
                     KNIDSVNITVPDHNHFIIAWNFIRKKKHVYLQKPMCHDVEEVRLLTEAAVRYGVITQL
                     GTQFASRPGDRAGVKLIRDGAIGRVKHAYLCSNRPGAVENYRLPGPRPPRGMTAPSNL
                     DWDLWLGTAPEREYSPEIYHPVKWRAWLDFGTGWSGDIGCHIFDAVWKGLDMKAPLSV
                     VAEVQESWKNSPERRADVWPQGDHITWMFPGNDRTDSDTFPVEWFDGEFYPPAEIMNL
                     YGGDEYPAESAMLVGTEGALLIPHGDMPVLLPEEKFASYPVPEFDPGNHYHSFVVACL
                     GGPKTESHFAQTGPMTEAILLGTVAVRLPGTVLEWNSGTMQITGHPDARKYLRRTYRK
                     GWKTKDF"
     gene            complement(3028..3741)
                     /locus_tag="PLZ75_10435"
     CDS             complement(3028..3741)
                     /locus_tag="PLZ75_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOS72849.1"
                     /translation="MNQFFAEFFGTAMILCLGSGVVANVLLNKTKGHNGGWIVITTGW
                     AIGVFTGVLIAADVSGAHLNPAVTLALVLAGKFSASLMPMYIAAQMLGAMFGSTLAWL
                     AYKKHFDATDDADYKLAVFSTIPNIRGYWYNLVTEIIGTYILALAVLYMAAPDVGLGA
                     LNALPVALVVLGIGVSLGGPTGYAINPARDLGPRIMHFILPVPGKRDSDWKYSWVPVV
                     GPMIGAALAAWMFVLFGGA"
     gene            complement(3835..>5222)
                     /gene="glpK"
                     /locus_tag="PLZ75_10440"
     CDS             complement(3835..>5222)
                     /gene="glpK"
                     /locus_tag="PLZ75_10440"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOS72850.1"
                     /translation="TQYFPSPGQVEHDPEEIWSTQAGVALEAITKAGLESTDIAAIGI
                     TNQRETTVVWNRKNGKPVYKAIVWQDRRTSGYCDQLKEEGHAEMIRQKTGLVIDAYFS
                     ATKLKWILDNVEGARDMAERGDLAFGTIDSWLIWNLSRGKLHVTDVTNASRTMLFNIN
                     TLRWDEDLLKLMDIPAGVLPEVRSSSEVYGTTSGQFSSSVPVAGIAGDQQAALFGQMC
                     IDPGMVKNTYGTGCFMMMNIGTKPTESKSRLLTTIAWKIGNQTVYALEGSIFIAGAVV
                     QWLRDSLGIIKTSADVEKLAAKADSSEGVYFVPAFAGLGAPYWNQHARGTITGLTRGS
                     TAAHIARAALDSIAYQTLDVLLAMEKDSDIDIYELRVDGGATVNSNLMQFQADLLQAN
                     VVRPKITETTALGAAYLAGLAVGYWSDLKEIRQQWQVDQIFYPGINSRKREELIQGWQ
                     KAVRAAESWAE"
BASE COUNT         1373 a         1307 c         1216 g         1326 t
ORIGIN      
        1 cccctgaata ccgtattgac cacatcatct tcacggaagc tgatcattaa agggggaaaa
       61 gtacaattgg ccggatcttt ccggaaatgt ccccttacgg atacaacaac gggcaatttt
      121 acttcagaac cgtcagtatt aaaataggtc agctgcccct cgtgaggctt aaccgtcccg
      181 gtacggtcgc tttcaatttc cgtgaaatct gcccgcagct ccatccgtat cacttcttcc
      241 gacatgaaaa gagtatcagt ttccgctgca gctgctgttg ccagaaagga tgaaaacaaa
      301 aggacacaga gtgatatttt aaacatattg aaaactgtgt taatcagatg ggtgcttttt
      361 cagaattaat cattaaactt gtagaatact gacgggtatc aatcatttat cccggcaaaa
      421 aatgaaacag atgaaaaaca tactttgttc aaaatataag atccggacaa gatgttttct
      481 gatttcgctg tccctggcca ttgcctcttc aaaccacctt gcggcatcag gaatagaatg
      541 cactgaaggc gatacagttt caatcaacga tggcccctat atctactttg actatgatat
      601 cctgaaagtc agatggatag aaaacaacaa cctggtggag gaagatctga cagcctccgg
      661 ctttgaagat atcaaggaaa aattcaatct gttatacaat ttccatgatc ttcagaacac
      721 ttttctgatt tctcctgcat tcaggcagaa ttacaggaac atcgacagca tagccctgat
      781 aagtgacgtt cacgggcagt atgacatata tataaacctt cttaaaaaga atggcataat
      841 cggtgaagat ctgtgctgga aattcggcaa aggccatctg gtagttctcg gagatgtttt
      901 tgacaggggt gataaggtaa cggaaatatt ctggcatctt ttcgggctgg aaaaacaggc
      961 cctgaaaagc ggaggcagag tccatttttt gcttggcaac catgaagcta tgattcttgg
     1021 ttgtgcaaca agctatatac atgacaaata taaaaaggtt gaaaagatta ccgggaagta
     1081 ttattgtgac ctttattccg ggaattcggt tctcggccgg tggctgcgca gcaaaccggt
     1141 tatcgcatcc atcaatgaca ttgtctttgt tcatgccgga ttatcagcgg atatcataaa
     1201 acagaaattg aaaatcgcaa aaatcaaccg cttgttctgt gaaaaaatca tcgcaaagga
     1261 ttattccctt atcgaaaaaa accaggatct cctcctcctc agtgatgaaa acggtccgct
     1321 atggtacagg ggatattttt ttgaccggag cttcaatgaa aacaggatag actcggttct
     1381 tgacttctat gaaaaaaaac atgtggttgt agggcacacg gtaacaaaag aaatagtatc
     1441 tgtattcaat aaaaaaatca taggctgtga tgcaggtatc atgtaccgca acccgggcga
     1501 aatgcttatt gttaaaaaag gagtgttcca caggggactg agcaacggag aaaggataaa
     1561 actataaaca ggttcctcat gattacttaa ccataataac cacttccaat ggctgaccag
     1621 aattattctt gtacaagaag gaaatttatt cagacagctg ctgtcgcagc tgttggtgca
     1681 cccttcatca tcagatgttc cccgggcgga agatcaccca acggcaggct gaatcatgcc
     1741 tgtatcggtg tcgggggaat gggatggcat gacctccata aattcatgga ggatcctaat
     1801 gtgcagatag tggctatctg cgatgttgat acggactatc tgcagaaagc ttccgaagtt
     1861 ctgcctgatg cccggaaata tactgactgg cgtgaattgc tggacaggga ggcaaaaaat
     1921 attgattcgg taaatattac agtacctgat cataatcatt tcatcattgc ctggaacttc
     1981 atccgtaaga aaaagcacgt ctacctgcaa aagcccatgt gccacgacgt tgaagaagtg
     2041 cgtctgctga cagaagctgc cgtcagatac ggggtgatca cccagcttgg cacccagttt
     2101 gcctccaggc cgggtgacag ggcaggggta aaactgatca gggacggggc aatcggcagg
     2161 gtgaagcatg cctacctgtg ttcaaaccgt cccggggcag tagaaaatta caggctgccg
     2221 gggcccaggc cgccgcgcgg gatgactgca ccgtcaaatc tcgactggga tctatggctg
     2281 ggaaccgctc cggaaaggga gtattcaccc gaaatatacc atcccgtgaa atggagggca
     2341 tggcttgatt tcggcacagg atggtcgggc gatataggct gccatatctt tgatgcagtc
     2401 tggaagggac tggatatgaa ggcgccgctg tcagtagtgg ccgaagtcca ggaatcatgg
     2461 aaaaattccc ccgagcgcag ggcagatgta tggcctcagg gagatcacat tacatggatg
     2521 tttcccggca acgacaggac cgattcagat acatttcctg tggaatggtt tgacggggaa
     2581 ttctatccgc cggctgaaat aatgaatctc tacggaggag atgagtatcc tgccgagtct
     2641 gccatgcttg tcggaaccga aggagccctg ctcatacccc acggtgacat gccggtactg
     2701 ctgcctgagg aaaaattcgc cagttacccg gtaccggaat ttgatccggg caatcactat
     2761 cacagtttcg tcgttgcctg tctgggaggt ccgaaaaccg aatcccactt cgctcaaacc
     2821 ggtcccatga cggaggctat cctgctcggg acggtggctg tccgcctgcc cggaacagta
     2881 ctggaatgga attccggaac gatgcagatc acgggacatc ccgacgcccg gaaatatctc
     2941 cgccgcactt acagaaaggg ttggaaaaca aaggattttt aattccttct ggccggatat
     3001 atttacgctg ctgaaagtaa tatgcttcta ggcccctccg aaaagcacaa acatccatgc
     3061 tgccagggct gctcctatca ttggccccac aaccggcacc caggaatatt tccagtcgct
     3121 gtcccttttt cccggaacag gcaggatgaa atgcataatc ctgggaccaa ggtcacgtgc
     3181 aggattgatg gcataacctg tcggaccgcc gagagagact cctataccca gaaccaccag
     3241 tgccacgggc agggcattca gtgcaccgag tcctacatcg ggtgcagcca tatacaggac
     3301 agccagggca agtatgtatg tcccgataat ctcggtaacg agattatacc agtaacctct
     3361 gatatttgga attgttgaaa aaaccgcaag cttgtaatct gcatcatccg ttgcgtcaaa
     3421 atgcttcttg taagccagcc aggcaagtgt tgaaccaaac atggccccca gcatctgggc
     3481 agctatatac atgggcatca gtgaggcgga aaacttgccc gcaaggacaa gagcaagcgt
     3541 aacggcagga ttaagatggg ctccgctgac atctgctgca ataagcactc cggtgaacac
     3601 acctattgcc catccggttg ttataacgat ccagccgccg ttgtgccctt ttgtcttgtt
     3661 cagtaatacg tttgcaacaa ccccgcttcc gaggcaaagg atcatggctg ttccgaaaaa
     3721 ttctgcaaaa aactggttca tacttacctt tttatttatc tgagttaata atgttatcac
     3781 accccgtctt cccggcttgt tcagggcggc cggaaccgaa tccctcctgt cctgtcattc
     3841 agcccacgac tcggctgccc tgactgcctt ctgccatccc tggatcaatt cctccctctt
     3901 tctgctgttt atccccggat agaagatctg gtcaacctgc cactgctgtc tgatctcttt
     3961 caggtcactc cagtagccca ctgccagtcc ggcaaggtat gcggctccca gtgccgttgt
     4021 ttcagttatt ttcggcctta ccacattggc ctgcagcaga tcagcctgga attgcatcag
     4081 attgctgttt accgttgccc cgccgtcaac cctcagctca taaatgtcaa tatccgaatc
     4141 cttctccatt gccagaagca catcaagagt ctgataggct atactgtcca gtgccgcacg
     4201 ggcaatatgc gcagcagtgg atccgcgtgt caggcctgta attgtacccc gtgcatgctg
     4261 attccagtat ggcgctccaa gcccggcaaa tgccggtaca aaataaacac cctcactgct
     4321 gtctgctttt gccgccagct tttcaacatc ggcagatgtc ttgataatgc ccaggctgtc
     4381 acgaagccac tgtaccacag caccagcaat aaaaatgctt ccctccagtg catatactgt
     4441 ctgattcccg atcttccacg ctattgttgt cagcagcctg cttttggatt ctgtaggttt
     4501 cgtgcctatg ttcatcatca tgaagcagcc ggtgccatag gtgttcttca ccatgcccgg
     4561 atcaatgcac atctgtccga aaagggctgc ctgctgatct cctgctatac ctgcaaccgg
     4621 tactgatgat gagaactgac cgctggtggt accataaacc tcacttgatg acctcacctc
     4681 cggcaaaaca ccggctggta tatccatcag tttcagaaga tcttcatccc acctgagggt
     4741 atttatattg aacagcattg tccgtgatgc gttggtaaca tcagtgacat gcagttttcc
     4801 ccttgacagg ttccatatca gccaggaatc aattgttccg aatgccagat cacctctttc
     4861 cgccatatcc ctcgctcctt caacattgtc taatatccat ttcagttttg tggcggaaaa
     4921 ataggcatct atgaccagtc cggttttctg acgtatcatt tctgcatgac cttcctcttt
     4981 cagctggtcg caatatccgg aagttcttct gtcctgccat actatggctt tatagacagg
     5041 tttgccgttt ttcctgttcc agacaaccgt tgtttccctc tggttcgtta tgccgatggc
     5101 agctatatca gtgctctcga gtcctgcctt ggtgatggct tccagtgcga ctccggcctg
     5161 tgtcgaccat atttcctccg gatcatgttc aacctgtccg ggactgggaa aatactgtgt
     5221 ga
//
LOCUS       DAOSPX010000229         5213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14505_length_5213_cov_4.352268, whole genome shotgun sequence.
ACCESSION   DAOSPX010000229 DAOSPX010000000
VERSION     DAOSPX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14505_length_5213_cov_4.352268"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLZ75_10445"
     CDS             <1..545
                     /locus_tag="PLZ75_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HOS72851.1"
                     /translation="RFTYGVNLDADWNNIFFSTFFQGVGKQDWYPSTEAEFFWGQYNR
                     PYNNIPKFHLGNMWTPENPNAYLPRTMSRAASSSTSRTLGVAQTRYLQNIAYVRLKNI
                     QMGYNLPSSITSKIRASAFKIYVSGENLWSWSPLYRIVKHIDPENTSGSDRILSGGTS
                     GDGYNYPMLKSMTFGISVVF"
     gene            564..2330
                     /locus_tag="PLZ75_10450"
     CDS             564..2330
                     /locus_tag="PLZ75_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS72852.1"
                     /translation="MKKIYLWFILASLLIAGCELEQYPESTTSRAAVFSSAQGLDLYA
                     NSFYNMLPGSTTLDAMTDYLAVKTIPNFLQPAFSATNSSGWDWGDLRNINYFLKYNTD
                     SRVPEEVRKNYNGIAKFFRALFYFEKVKRFGDVPWINIPLDVSDTIMLYGPRNSRTLI
                     MDSVLADINYACANITSTVDASRSLITKYVAYGLKSRICLFEGTFRKYHTELGLESTA
                     NTWLNDAASAAKTVMDAGTYSIYTSGGTTKAYRTLFTSSAPVASEVMLAAVMDLTLNR
                     LHDANWWWTSGTYGSKASFIRSFINTYLMEDGTPFTNTPGWQTMLFKDEVKGRDHRLQ
                     QTIRMGDYKRISDGVQIPGPPVFSYTFTGYQPIKWTLDDMYYDTRDLNINSISLMRYA
                     EMLLNYAEARAELGTLSDADWASTIGVLRSRAGITGGLSAKPTEVDPYLQSTYFPDIS
                     DPVILEIRRERGIELSLEGVRFPDILRWKRGELMKMEWNGFYVPELVTPMDLNEDGIL
                     DVAFFQGTKPSPSFKGVEYVDVSAMIGTNQNSQRLKNNTYGELTWMNNLVRAWEDKHY
                     YYPIPLNDILQNPNLKQNPGWQ"
     gene            2404..3270
                     /locus_tag="PLZ75_10455"
     CDS             2404..3270
                     /locus_tag="PLZ75_10455"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOS72853.1"
                     /translation="MKHIKSSLSFLTLILTSFLLSNQAYGQSKKETPFTLITYNIRYN
                     NPGDGVNAWPARKDKVAGLLRFHKADIFNVQEALIEQVNDLKISFPDFDYYGLGRDDG
                     KEAGEHMSVFFRKSRFEKLAEGTFWLSETPDKPGMGWDAACNRTCTWLKLRDKITKKI
                     FFVFNTHLDHRGTKAREEGVALILNRITEINRENLPLILTGDFNLVKESNPVSSILTT
                     LSDSRDKSLTASYGPDATSGGFAVSDRGRTIDFIFINDKIKILRHGVLSDSFGMFYPS
                     DHKPVLAEIQIP"
     gene            complement(3461..3985)
                     /locus_tag="PLZ75_10460"
     CDS             complement(3461..3985)
                     /locus_tag="PLZ75_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HOS72854.1"
                     /translation="MKRILVDMDGVLADVYSRFFDLHEQATGSRLSPSDVAGMPESEA
                     FPEQVSWVSAPGFFRHLPVMPGSIEGMKKLNSVFDIVVISLATEFPQSLTDKQLWMHD
                     HFPFISWKQLVFCGNKNLLHADIMIDDHPKNLDHFPGETIIFTQPHNINIHDTRHIRI
                     SSWLEAEKLLFKKV"
     gene            4111..>5213
                     /locus_tag="PLZ75_10465"
     CDS             4111..>5213
                     /locus_tag="PLZ75_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72855.1"
                     /translation="MTTRREFFQKAALGAAGLTLGARSYSRVIGANDRVRVGIVGYSD
                     RFRGSLHPCFMSLAKEMNFEFTALSDIWRLRREDAARFYKKLGINIRLARNNEELYNG
                     KDVDAVIISTADFQHALHTKEAVEAGCDAYVEKPFAETMADARVARKAVEETGKIVQI
                     GSQRRSAGNYIAANEYIKSGKFGRIVMVEMTWNVNQPGRWRRPNVVPLLKASDTDWKR
                     YQMNRPAADFDPRKYLEFRLFWPYSSGIPGQWMAHQIDTVHWFTNISHPRTVAANGGI
                     YLWKDGRSNFDTMTAVMEYVDPVNPDNAFQVVYSSRFTNSAGGVKELYYSNGGMLNLD
                     TNKISSEGGMTEREARAMGMNANLLEPFELPGL"
BASE COUNT         1561 a         1068 c         1195 g         1389 t
ORIGIN      
        1 cccgctttac ctacggagtc aatcttgatg ctgactggaa taatattttc ttctcaacct
       61 ttttccaggg agtagggaag caggactggt atccgagtac tgaagctgaa ttcttctggg
      121 gtcagtataa ccggccatac aacaatatcc caaaattcca tcttggtaat atgtggacac
      181 cagaaaatcc caacgcgtat ctgcccagga ccatgtcgcg tgcggcatcg agctccacga
      241 gcaggacact tggtgttgcc cagacaaggt atctgcagaa tattgcctat gtaagattga
      301 aaaatattca gatgggttac aacctgccat ctagtattac ttcaaagatc agagccagcg
      361 cgtttaaaat atatgtttca ggtgagaacc tctggtcgtg gtcgccattg tacaggattg
      421 taaagcacat agaccctgaa aatacttcag gttctgaccg catattatca ggaggaacat
      481 ccggagacgg gtataattat cccatgctga aaagtatgac ttttggaata tcagttgtat
      541 tttaactttt aaaataattg atcatgaaaa aaatatattt gtggtttata cttgcaagtc
      601 ttctgattgc cgggtgtgaa ctggagcaat atcccgaatc tacgacatca cgtgctgctg
      661 tattcagcag tgcccaggga cttgatctgt atgccaattc attttataat atgcttcccg
      721 ggtctacaac acttgatgcg atgactgatt atcttgcagt gaaaacaatc ccgaattttc
      781 ttcagccggc attttctgca acaaacagtt cagggtggga ctggggtgat ctaagaaata
      841 ttaattattt cctgaaatat aataccgatt ccagggtccc cgaggaagtc cggaaaaatt
      901 ataatggtat agcaaaattc ttcagggcac tgttttattt cgagaaagta aaacgcttcg
      961 gtgatgttcc ctggataaat atacctcttg acgtgtcaga taccattatg ttatatgggc
     1021 cgcgtaattc gcggaccctg atcatggatt ccgttcttgc agatataaat tatgcctgtg
     1081 ctaatattac atcaactgtc gatgcttcca ggagtttgat aacaaaatat gttgcatacg
     1141 ggctgaaatc aagaatatgc ctgtttgaag gaactttcag aaaatatcac actgaattgg
     1201 gccttgagtc cacggcaaat acctggctta atgatgctgc atctgctgcc aagaccgtaa
     1261 tggatgccgg tacctattcc atctatactt caggaggaac gacaaaagct taccggactc
     1321 tgttcacgag ctctgcaccg gttgcatccg aagtcatgct ggctgctgta atggatctca
     1381 cactaaaccg gcttcatgat gccaactggt ggtggaccag cggtacgtac gggagcaaag
     1441 ccagttttat ccgcagcttt ataaatactt acctgatgga ggacggcact ccttttacaa
     1501 atacacccgg gtggcagacc atgcttttta aagatgaagt taaaggaaga gatcacaggc
     1561 tgcaacagac tattcgaatg ggtgattata aaagaataag tgatggtgtt cagattccgg
     1621 gccctcctgt gttttcctat acctttaccg ggtatcagcc tatcaaatgg actctcgatg
     1681 atatgtatta tgataccaga gaccttaaca taaactccat atccctgatg agatacgcgg
     1741 agatgcttct taattatgcg gaagccaggg cggaactggg taccctgtca gatgcggatt
     1801 gggcttcgac aataggagta ctccgttcaa gagccggcat tacaggaggc ctttctgcaa
     1861 aacccactga agtcgatcct tatctccagt caacctattt cccggacatc agcgatccgg
     1921 taattcttga aataaggaga gaaagaggta ttgaactgag cctggaggga gtccgtttcc
     1981 ctgatatact gagatggaag agaggtgaac tgatgaagat ggaatggaac ggattctatg
     2041 taccggagct tgtaacacca atggatctga atgaagacgg aattcttgat gttgcatttt
     2101 ttcagggaac aaagccatct ccctcattta aaggtgtgga atatgtagat gtttcagcga
     2161 tgatcggaac aaatcagaat tcacaaaggc ttaaaaataa tacttacgga gaactcacct
     2221 ggatgaataa tctggtaaga gcatgggagg ataagcatta ttattatcct attcccctaa
     2281 atgatatact gcagaatccg aatctgaaac aaaacccggg ctggcaatag aaaatattgt
     2341 ttgtaattaa aattaatcgc gatgaaccgg ttcatcgcga ttaattttat aaaacttatt
     2401 actatgaaac acataaaatc atcattatca tttctgacac taattctgac ctcgtttttg
     2461 ctttcaaatc aggcatatgg ccaatcaaaa aaagaaacac ccttcacatt aataacttat
     2521 aatataaggt ataacaatcc cggcgatggc gtaaatgctt ggcctgcaag aaaagacaag
     2581 gtcgcgggcc tgctaagatt tcataaggca gatatcttta atgttcagga agctctgatc
     2641 gaacaggtca atgatctgaa aatcagcttt cccgattttg actactacgg actcggccgg
     2701 gacgacggta aagaagcagg ggaacacatg tcggtttttt tcaggaaatc cagatttgaa
     2761 aaactggcag aaggaacatt ctggctgagt gaaacacccg ataaacccgg gatgggttgg
     2821 gatgcagcct gtaatcgtac atgcacatgg ctgaaactca gggataagat cacgaaaaag
     2881 atattctttg tatttaatac ccatctcgat cacaggggga cgaaagcacg tgaagaagga
     2941 gttgcgctga tactcaatcg gataacagaa ataaacaggg agaatcttcc tctgatactg
     3001 accggcgatt ttaatctggt gaaagaatca aaccctgtca gttcaatatt gacaaccctg
     3061 agtgacagcc gtgataaatc attgacagca tcctatgggc ctgatgctac aagcggagga
     3121 ttcgccgtca gtgacagggg caggacaatt gatttcattt tcataaatga caaaatcaaa
     3181 atactcaggc acggggtttt gtcagattca ttcggcatgt tttatccttc tgatcataaa
     3241 ccggttctgg cagagattca aataccttaa aatttctggt tatctttctc gtggaaagaa
     3301 catcttaaca gaacaactga ggcctgatgc ttttaatgct gaagtaaagt gttccggacg
     3361 gtttgatgaa ttttattccc ttaattgccg ggacacggga ttcactcatg gagatatgga
     3421 gggacaataa acatttaaag aaatattctg actggtaaaa tcaaactttc ttaaaaagaa
     3481 gtttttctgc ttccagccat gaagagattc ttatgtgccg tgtgtcatgg atgttaatat
     3541 tgtgaggctg tgtgaaaatg atggtttcac cgggaaaatg atcaagattt ttggggtgat
     3601 catctatcat tatatcagca tggagaaggt ttttgttccc gcaaaaaacc agctgtttcc
     3661 atgatatgaa ggggaaatgg tcatgcatcc acagctgctt gtcggtaaga ctctgaggaa
     3721 attccgtcgc cagtgagatt accacaatat caaatactga attaagtttc ttcattcctt
     3781 caatacttcc gggcataacc ggaagatgtc tgaaaaaccc gggtgccgag acccagctta
     3841 cctgttccgg aaaagcttcg gattcaggca tgcctgcaac atccgacggg gaaagcctgc
     3901 tgcccgtcgc ctgttcatgc aggtcaaaga acctggaata tacatcggca agcactccgt
     3961 ccatatcaac aaggatgcgt ttcataacaa ctattgtgaa ttgaatggaa taaaattaaa
     4021 aaaagcagtt gcattattac ctaatgattg tgaattaaat tatttttact ttcttgagca
     4081 tgctattcca tgaactttaa ataaaaaatt atgacaacca gaagagaatt ttttcagaaa
     4141 gctgccctgg gcgctgcggg acttaccctt ggcgcaagaa gctattcacg tgtgattggt
     4201 gcaaacgaca gggtaagagt aggtatagtc ggttattccg accgtttcag gggttcgttg
     4261 catccatgct ttatgagtct tgcaaaggaa atgaattttg aattcacagc tctttcagat
     4321 atatggagat taagaagaga ggatgctgcg cgcttctata aaaaactggg gatcaacatc
     4381 aggcttgccc gtaataatga ggaactgtac aacggcaagg atgtggatgc ggtaataatc
     4441 agcacagcag atttccagca tgcactgcat accaaagaag ctgttgaagc cggatgtgat
     4501 gcctatgttg aaaagccctt tgccgaaact atggcagatg cccgtgttgc cagaaaggca
     4561 gttgaggaaa cgggcaagat agtccagata ggttcacagc gccgctccgc aggaaattac
     4621 attgctgcaa atgaatatat caaatcagga aagttcggca ggatagttat ggttgaaatg
     4681 acatggaacg taaatcagcc cggaagatgg agaaggccga atgttgttcc cctgctgaag
     4741 gcttccgata ccgactggaa aagataccag atgaacaggc ccgctgctga ttttgatccc
     4801 agaaaatatc tggaattcag gctgttctgg ccatactcct ccggcattcc gggtcagtgg
     4861 atggctcatc agatcgatac agtccactgg ttcacaaata tatcccatcc gcgcacggtt
     4921 gccgcaaacg gcggaatcta tctgtggaag gacgggcgct ccaatttcga caccatgact
     4981 gcggtgatgg agtatgttga tcccgtaaat cctgataatg catttcaggt ggtttattcc
     5041 tcaaggttca ccaactccgc cggtggcgta aaggaacttt actattcaaa tggcggaatg
     5101 ctcaacctcg acaccaacaa aatatcttcc gagggaggaa tgactgaaag ggaagccagg
     5161 gcaatgggaa tgaatgccaa tttgctggaa ccgttcgagc tgccgggcct gag
//
LOCUS       DAOSPX010000230         5209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14518_length_5209_cov_4.620295, whole genome shotgun sequence.
ACCESSION   DAOSPX010000230 DAOSPX010000000
VERSION     DAOSPX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14518_length_5209_cov_4.620295"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLZ75_10470"
     CDS             complement(<1..833)
                     /locus_tag="PLZ75_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72856.1"
                     /translation="MTKRRDFIKKSAMGTAAIAIGSAGFSAKSYGSIRGANERITVAV
                     IGIRGQGGGHINTWCRMKDSQNVRLKTICDADEEVFAEKVQAAQKITGEAPRTEWDMR
                     KVFDDKDIDAVSFATPNHWHALGTVWACQAGKHVYVEKPASHNVWEGRKMVEAARKYN
                     VRVQVGFQNRSIPNVMEAIRFMHNGGIGKVYLARGLCYKPRDSFGIAPDSQPPASLHY
                     DIWLGPAQWRPYNEKRGHYNWHWFWDTGNGDTGNQGPHQFDIARWGLNKNEHPVKIYS
                     AG"
     gene            complement(885..1928)
                     /locus_tag="PLZ75_10475"
     CDS             complement(885..1928)
                     /locus_tag="PLZ75_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72857.1"
                     /translation="MGNQFNRREFIRAAGAGAMALGLAGRSFPAAGSRPQGSKRIGII
                     GLDTSHSTAFTKTLNDPSAGPQFQGYKVVAAYPRGSRDIQSSVERIPGYTEDVKKMGV
                     EIVESIDELLRKVDFVLLETNDGRLHLEQAIPVMKAGKRMFIDKPVAASLDDTIAIFN
                     AAEYYKTPLFSASSLRYIAGAKEIAEGAIGKVIGAEVYSPATLEKTHPDLYWYGVHGV
                     ETLFTIMGTGCKTVSRAFTPGTDLVTGIWEDGRIGTMRGIRTGRSGYGGVVYGEKEIR
                     TLGSYAGYDPLLIEIVRFFTTGVLPVRPEETLEIFAFMTAAEESRQKGGAPVQMEKVI
                     EKARKKAAKINFK"
     gene            complement(2051..4267)
                     /locus_tag="PLZ75_10480"
     CDS             complement(2051..4267)
                     /locus_tag="PLZ75_10480"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS72858.1"
                     /translation="MQEKNPSGKSPVSKVPVSRKPFDAFLLPIAVLLLIIFITGTVNA
                     QNDLDVIRNSWMEHSDASNSLYHHLADQCYDLIDKREKAISGLNSLADWQERQKFIRE
                     TLMDIVGPFPEKTPLNARTVKTIDKGSFRVEHVIYESLPGFYVTSSLYIPSGLRRRDK
                     APAIIYCSGHTEEGYRSATYQHVILNLVTKGFIVFAFDPVGQGERLEYYDPATGKSSV
                     GGPTKEHSYPGAQAFITGSSQARYMIWDGIRAVDYLLSRREVDPARIGITGRSGGGTQ
                     SAYIAAFDNRILAAAPENYLTNYRRLLQSIGPQDAEQNMFHFLYRGLDHPDLLLVRAP
                     KPALMITTTRDIFSIQGAMETEKEVAGLYRLYGETENFSRIEDDDGHASTKKNREAMY
                     AFFRQHLNNPGSIEDERISPLTAEELRVTQTGQVSTSLGGETVFSLNRKEAGIRLNEL
                     NEARRNPENFYAGIAEPAKRMSGFREPSLDDLPVFTGRIARDYYNIEKYFIKGEGDYV
                     IPYLLFRPVLWNNRGVFYLNPAGKSAGAGPGGDIERFLRQGFAVMAPDMAGCGENGPG
                     SFRGDAYFDGSSHNLWYASLLAGRSITGIHTGDVVKLIRVLRKECPYAEIIGFARKEM
                     APVLLHTAIIDRNISSVILAEPYSSFRSVVMNKSYDTNLILSTIPAGLTAYDLPDLAA
                     ALAPRKLFIAGMTDGNGTVTDVQDDISIIRNVYSLRNAADRIRIEADLSALDSWIE"
     gene            4387..4944
                     /locus_tag="PLZ75_10485"
     CDS             4387..4944
                     /locus_tag="PLZ75_10485"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOS72859.1"
                     /translation="MKNKKAKKESRILRHKDLLITASAGILVFMFFSFFYANHLRFAE
                     QLQLFLCTGNYFTEKLLLPGGLNGWTGSFLTQFYNIPAAGAFIISILFISIQLLTRAL
                     IREFNDGRKNTYLLSFLPALAAGLMLCNELYPLSAVTGFTASLAAAWVYTGIKNEKRR
                     FAAGVILIPLIYYILGGAFLSMVLI"
     gene            4992..>5209
                     /locus_tag="PLZ75_10490"
     CDS             4992..>5209
                     /locus_tag="PLZ75_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72860.1"
                     /translation="MNKERINKAGTGTGTGRTGTETAGKGRSWAKRSKLCPILLYIFL
                     SAAVPLLVRYFFVFQPAGQAFFTEFYYN"
BASE COUNT         1295 a         1328 c         1126 g         1460 t
ORIGIN      
        1 ccggcggaat atatcttcac cggatgctca ttcttgttga gtccccagcg tgctatgtca
       61 aactgatgcg ggccctggtt acccgtgtcg ccgttgcctg tatcccagaa ccagtgccag
      121 ttgtaatgac cccgtttttc attgtagggt ctccactgtg ccggtccgag ccatatgtca
      181 taatgaagcg aagccggcgg ttgactgtca ggagcaattc cgaacgaatc acggggcttg
      241 tagcaaagtc ctcttgcaag gtacactttt ccaattcctc cgttgtgcat gaacctgatg
      301 gcttccatga cgttggggat tgaacggttc tggaacccta cctgtacacg gacattgtat
      361 ttcctggcag cttcaaccat cttgcggcct tcccacacat tatgtgatgc aggtttttca
      421 acataaacat gctttcctgc ctgacaggcc catacagtac ccagggcatg ccagtggttc
      481 ggggttgcga atgaaacagc atcaatatcc ttgtcatcaa acacttttct catatcccat
      541 tccgtccgcg gggcctctcc ggtaattttc tgagctgcct ggaccttttc tgcaaaaact
      601 tcctcatcag catcgcagat cgtcttaagg cgtacattct ggctgtcttt catacgacac
      661 catgtgttga tatgtccgcc tccctgtccg cgtatgccta taacggcaac agtgatacgc
      721 tcattggcac ccctgatgga accgtaggat tttgcactga atccggcact gccaatagct
      781 atggcagcag ttcccattgc gctcttttta atgaaatctc ttcgttttgt catttcctga
      841 ttcgctttaa aattaaaatc tgattattct gttggtaaac tggtttattt aaaattaatt
      901 ttcgcagcct tcttcctggc tttctcaatt actttttcca tctgaacagg agcgcctccc
      961 ttctgcctgc tctcctcagc tgctgtcata aaggcaaaga tctcaagtgt ttcctccggc
     1021 ctgaccggca aaacacccgt ggtgaagaat ctcactatct ctatcagcag gggatcatag
     1081 ccggcatagc ttcccagggt tctgatttcc ttttccccgt ataccacgcc tccgtacccc
     1141 gatctgcctg tccttattcc tctcatggta ccaatcctgc catcctccca tattcccgtc
     1201 accaggtcgg tacccggggt aaatgccctg gatacggttt tacagccggt tcccataatg
     1261 gtaaagagtg tttcaacccc atggactccg taccagtaaa gatcaggatg ggtcttttcg
     1321 agagttgccg gactatagac ctcagcacca atcaccttcc cgatggctcc ctccgcaatt
     1381 tctttggctc cggcaatata tctcagggac gaagcggaaa aaagcggggt cttataatat
     1441 tccgctgcat tgaagatcgc aatggtatca tccagggacg cagcaacagg cttgtcaata
     1501 aacattctct tacctgcctt catcacaggt atggcctgtt ccagatgaag cctgccgtca
     1561 ttggtttcaa gaagaacaaa atcaaccttc cggagaagtt cgtctatgga ttccacaatc
     1621 tcaactccca tttttttcac gtcctccgtg taaccgggga tccgttccac acttgactgt
     1681 atatccctgc tgcctctcgg ataagccgca acaactttat agccctgaaa ctgcggaccg
     1741 gctgaaggat cattcagtgt cttggtgaac gcagtgctgt gagatgtgtc aaggccgatt
     1801 atcccgatgc gctttgagcc ctgtggacgg gaacctgctg caggaaaaga tcttcctgca
     1861 agtccaagtg ccatggcacc cgcgccggct gccctgataa attcacgtct gttaaattga
     1921 tttcccatat ttttagaatt gaaatttata atcggggtaa ataaaatcag tcgtatgttc
     1981 tgttaaaatt aatttctttt agctcatcac attcattcca tacaggattt tatttgttaa
     2041 aagaagttaa ttattctatc catgaatcga gggctgagag atctgcttcg atcctgattc
     2101 tgtcagctgc attccgcaat gaataaacat tccggataat actgatatca tcctgaacat
     2161 ctgttacggt accgtttcca tcagtcatcc cggcaataaa aagcttacgg ggagcaagtg
     2221 ccgcggcaag gtcaggcagg tcataggcag tgagtccggc aggaatcgta ctgagaatga
     2281 gatttgtatc ataggattta ttcattacca ccgacctgaa tgaggaatag ggctcagcca
     2341 gaatcactga tgaaatgttc ctgtcgataa ttgcggtgtg gagaagcacg ggggccattt
     2401 cttttctggc aaagccgata atctcggcat aggggcattc cttccttaac actcttatca
     2461 gcttgaccac atctcccgta tgtatcccgg taatgcttct ccctgcaagc aatgaagcat
     2521 accacagatt gtgggatgaa ccgtcaaagt aagcatcgcc gcggaatgat cccgggccgt
     2581 tttctccgca tccggccata tcgggcgcca taacagcgaa tccctgcctc aggaatcttt
     2641 caatatcgcc tcccggcccg gccccggctg atttaccggc aggattgaga taaaacacac
     2701 cccggttatt ccataataca ggccggaaca ataagtaagg aatgacataa tcgccctccc
     2761 cctttataaa atacttttca atattgtagt aatccctggc aatacggccg gtaaacaccg
     2821 gcagatcatc aagggaaggt tcacggaatc ccgacattct tttcgccggt tctgcaatgc
     2881 cggcataaaa attttcagga ttgcgtcgtg cttcattcag ctcattcagc cgtattccgg
     2941 cctccttcct gttaaggctg aaaacagttt ctcctcccag cgatgttgat acctgtcctg
     3001 tctgagttac cctgagttcc tctgccgtga gcggactgat cctttcatct tcaatacttc
     3061 cgggattgtt aagatgctga cggaaaaaag catacatggc ttcacgattc ttttttgttg
     3121 aggcatggcc gtcatcatct tcaatccttg aaaaattttc tgtttcaccg tacaacctgt
     3181 acaaccctgc aacctccttt tcggtttcca ttgcaccctg aatgctgaat atatcacggg
     3241 ttgtggtgat catcaatgcc ggcttcgggg ctcttaccag aagaaggtca ggatgatcaa
     3301 gcccccggta aagaaaatgg aacatattct gctcggcatc ctggggacct atcgactgaa
     3361 gaagccttct gtagttcgtc agatagtttt caggagctgc cgcaagaatg cggttatcga
     3421 atgctgcaat atatgccgat tgcgtgccgc ctcccgacct gccggtaata ccaatccttg
     3481 caggatcaac ctccctgcgc gaaagaagat aatcaaccgc cctgataccg tcccagatca
     3541 tataccgcgc ctgtgagctt ccggtaatga aagcctgtgc tcctggatat gaatgttcct
     3601 tggtgggtcc accgacagat gattttcctg tggcaggatc gtaatattcc aggcgctctc
     3661 cctgtccgac aggatcaaag gcaaaaacga tgaaaccctt ggttaccagg ttcagaatta
     3721 cgtgctgata ggtcgcgctc cggtaaccct cttcagtatg tccgctgcag tagataattg
     3781 ccggcgcctt atcccgccgc cttaatcctg aagggatata taatgaggag gtgacataaa
     3841 atcccggaag ggattcatag atcacatgct ccaccctgaa agacccctta tcaatggttt
     3901 ttaccgttct ggcattgagt ggtgtttttt cagggaaagg tccaactata tccatcaggg
     3961 tttccctgat gaatttctgt ctttcctgcc agtctgccag agaattcagc ccggaaatgg
     4021 ccttttccct tttatcaata agatcatagc actgatctgc caggtggtgg tacagtgaat
     4081 tggatgcgtc gctgtgttcc atccatgagt ttctgatcac gtcaagatcg ttctgggcat
     4141 tcactgttcc tgtaataaag attatcagca acagtactgc gatcggaaga agaaatgcat
     4201 caaatggttt ccggctgacc gggaccttac taaccggact tttgccggaa ggattttttt
     4261 cctgcatggt aagagagtta ttcagtttta cggtttaatg atattacaca aaaataatta
     4321 ttttaatata ttagttttgt atttaatcaa tgctgaacag aactgagttc agccagccgc
     4381 ggcctgatga aaaacaagaa agcaaaaaaa gaatcccgga tattaaggca caaagactta
     4441 ctgataacag cctctgccgg aatccttgtt tttatgtttt tttctttttt ctatgcaaac
     4501 caccttcgct ttgcggaaca gctgcagctc ttcctttgta caggcaatta ttttactgaa
     4561 aaactactgc tccccggggg tctcaatggc tggacaggct cattccttac acagttttac
     4621 aatattcccg cagccggggc attcatcatc agcattctgt ttatctcaat acagctgctc
     4681 acccgggcac ttatccggga attcaatgac ggaagaaaaa acacttatct tctctcattc
     4741 ctgcctgccc ttgccgccgg cctgatgctc tgcaatgaat tatacccgct ttcagcagta
     4801 acaggattca ccgcctccct tgccgcagca tgggtttata cagggataaa aaatgaaaaa
     4861 cggagatttg ctgccggcgt tattcttatt ccgctgattt attatattct gggaggagca
     4921 tttttgtcca tggtgctgat atgacagcat atgaaattat cctgttaaag aatgccgctc
     4981 tggcgcacgg catgaataaa gaaagaatta ataaggctgg aacaggaaca ggaacaggaa
     5041 gaacaggaac agaaacagca ggaaagggaa gatcatgggc aaaaagatca aagctgtgcc
     5101 cgatcttgct ttacatattc ctgtcagcag ccgtacccct tcttgtcaga tatttttttg
     5161 tgtttcagcc tgccggacag gcctttttca cagaatttta ttataacat
//
LOCUS       DAOSPX010000231         5165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14659_length_5165_cov_4.902740, whole genome shotgun sequence.
ACCESSION   DAOSPX010000231 DAOSPX010000000
VERSION     DAOSPX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14659_length_5165_cov_4.902740"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLZ75_10495"
     CDS             <1..468
                     /locus_tag="PLZ75_10495"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOS72861.1"
                     /translation="KALDLNFDGLMLESHIQPEKALSDSAQQITPGELHELLSRLILR
                     NPSTSDPKLLDILGELRQQIDVFDDHLIDLIERRMEVAEKIGHYKKENNITILQSARW
                     DEIVKRAMTKGLSKGLSREMIDSIFKAIHQESINKQMKIMNNGIDINIADKNI"
     gene            473..1723
                     /gene="aroA"
                     /locus_tag="PLZ75_10500"
     CDS             473..1723
                     /gene="aroA"
                     /locus_tag="PLZ75_10500"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOS72862.1"
                     /translation="MDYFVVPSEISGSVTAPPSKSMTQRAIAAALLAKGESIITNPSD
                     CDDSLAAINIARALGADVTREPGILRISGAGKIREQLLNCGESGLAIRMFSPVAALNV
                     EEIIMTGTGSLVKRPMSIIEDALRQFGVKCKSEGGFLPLTIQGPLRGGHCEIDGSLSS
                     QLLTGLLMALPVTEKDSIIIVNNLKSKPYIDMTLQVLKSFGINILKTGYSRFEIPGNQ
                     KYKAGKFEVEGDWSGGAFLLVAGAINGDIRIEGLRADSLQSDKAILNVLDSAGVRTTI
                     LENSITVRKSPLKAFFFDATESPDLFPPLVTLAAFCRGKSTIKGVSRLIHKESNRAEA
                     LITEFGRIGIRIETSDDNMIVSGGSVKGGEVSSHGDHRIAMAAAVAALGSSGKILIKN
                     SGCVAKSWPLFFEQLRRLGTMIWE"
     misc_feature    1827..1930
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            1970..3331
                     /locus_tag="PLZ75_10505"
     CDS             1970..3331
                     /locus_tag="PLZ75_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOS72863.1"
                     /translation="MKKNTKILLSEREMPRQWYNIVADMPNKPAPPLNPATREPIDPA
                     ALAAVFPMELIRQEMSAERYIDIPDEVLNLYKIYRPSPLHRALNLEKALGTKSRIYYK
                     YEGGNYSGSHKANTAIAQAYYNKKEGIRRITTETGAGQWGSAMSFACNHFGLELLVFM
                     VSVSFHQKPGRKIMMNTYNAKVVPSPSTMTAVGRQILEKDPDSPGSLGIAISEAMEIA
                     VQDPYTKYSLGSVLNHVSLHQTIIGQEAMIQMEKAGDYPDVVIGCFGGGSNFSGIAFP
                     FIRENIVSGRKTRFVAVEPSSCPKLTKGRFLYDFGDSAGMTPLLPMYTLGHNFIPEKI
                     HAGGLRYHGAGVLVSQLLKDGFVEAKAKMQRECFEAGVFFARNEGILPAPESNYAIAA
                     ALDEARQSDIEGTSRVILFNLSGHGHMDVSSYQKFFEDNLPDHEMTQEEVDKFIAASG
                     LEG"
     gene            complement(3377..4168)
                     /locus_tag="PLZ75_10510"
     CDS             complement(3377..4168)
                     /locus_tag="PLZ75_10510"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HOS72864.1"
                     /translation="MTKAIRTLFSMVFVAVLLFPAGGCREQIPQMLYEIKIYDVSGPG
                     QAERTERYISGALKPVLRRSGIEGVGVFKPLESDTAASGKYIYVFIPYETADKYFSMK
                     VVLEKDADYQKRGSDFLNAPHDNPPFQRYESILLKAFAGMPRFSTPSFATDRSERVYE
                     LRSYESATEYKAGKKIDMFNNEEIALFEKLGFNAVFYGQVLAGSHMPNLMYMTTFQNM
                     KTHDEKWEEFRNDPDWIRMRDKEEYRNTVSRNTQYLLYPLSCSDF"
     gene            complement(4214..>5165)
                     /locus_tag="PLZ75_10515"
     CDS             complement(4214..>5165)
                     /locus_tag="PLZ75_10515"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS72865.1"
                     /translation="IKGGQKVDVMIGGKLFTSYQYPSNMEKPYLWPVNAPDGSVVTRG
                     FPLEPRPRERVDHPHHIGIWFNHGDVNGLDFWNNSSAIPAEKKDAYGHIAVREIVKVK
                     NGKKAVLEVISDWKDNKGNTLLTENTQYIFSGTKVSYTIDHISTLTAAEGPVTITDNK
                     EGMFAIRVDRAFEMPTNQPAVYTDSQGNPTTVRATDNEGVTGMYTSSSGKRGDDVWST
                     RNDWVILSGTKNNVLTSFGFWDHPKNIGYPGHSHARGYGLFSTNNLGSQAYNKDDEKI
                     VLTLGKGESVTLRHRFYIQSGKEITAGQANSIFREFSALY"
BASE COUNT         1421 a         1226 c         1236 g         1282 t
ORIGIN      
        1 aaagcgctcg acctcaactt cgacggttta atgctggaat cacacatcca gcctgaaaag
       61 gcgcttagcg attcagccca gcagattact cccggtgagc ttcacgagct cctcagcagg
      121 ctgatactcc gcaatccctc aacatccgat ccgaagctgc ttgatatcct cggggaatta
      181 cggcagcaga tcgatgtttt tgatgaccat ctcattgatc ttatcgaacg taggatggag
      241 gtggcggaaa agatcggcca ctacaagaag gagaataata tcactatcct tcagagcgca
      301 cgctgggatg aaatagtgaa aagggccatg acgaagggcc tgtctaaagg attgagcagg
      361 gagatgatag attccatatt caaggccatc catcaggaat ccatcaataa acagatgaaa
      421 atcatgaata atggcattga cataaacata gcggataaaa atatctgagc tgatggatta
      481 ctttgttgtt ccctcggaaa taagcggatc cgtaaccgcc cctccttcga aaagcatgac
      541 ccagcgtgcc attgcagccg ccctcctggc aaagggagaa agcattataa cgaatccttc
      601 ggattgtgac gattcacttg cagcaataaa tattgccagg gcactgggtg cggatgtgac
      661 ccgggaaccc ggtatactca ggatcagtgg agccggaaag atcagggaac aactgctgaa
      721 ctgcggggaa tcgggacttg ccataaggat gttttccccg gttgcggccc tgaatgtgga
      781 ggaaataatt atgacaggca caggttccct cgtgaaaagg ccgatgtcaa taattgaaga
      841 cgcactcaga cagttcgggg tgaaatgtaa aagtgaggga ggcttccttc ctctcaccat
      901 ccagggaccc ctgaggggcg gacactgtga aatagacgga tccctgagtt cccagctgct
      961 cacgggactg ctgatggctc tcccggtaac tgaaaaggat tcaataataa ttgtaaacaa
     1021 ccttaaaagc aaaccctata ttgacatgac cctgcaggtt ctgaaaagct tcgggatcaa
     1081 tatactgaaa acgggctaca gccgttttga gataccaggg aatcagaaat acaaggcagg
     1141 aaaattcgag gttgaaggtg actggagcgg aggtgcattt ctactggtgg ccggggcaat
     1201 aaacggcgat ataaggattg agggtctcag ggccgacagc ctgcagagcg ataaggcaat
     1261 actgaatgtc ctcgacagtg ccggagtaag gacaacaata ctggagaatt caataacagt
     1321 aagaaaatca ccactcaaag ccttcttttt tgatgcaacc gaatcacccg acctcttccc
     1381 tccccttgta accctggcag cattctgcag gggcaaatcg acaataaagg gcgtgtcccg
     1441 cctgattcac aaggaaagta accgggccga ggccctgatt accgaattcg gcagaatcgg
     1501 catcaggatt gaaaccagtg acgacaatat gatagttagc ggaggctccg taaagggcgg
     1561 tgaagtcagt tcacacggcg atcaccggat tgcaatggcg gctgcagttg cagcactggg
     1621 aagttcgggg aaaatcctga taaaaaattc cggatgtgtc gcaaaatcct ggcccctgtt
     1681 ctttgaacaa ctgcggaggc tcggaacaat gatctgggaa tgattccgga cggcagacgg
     1741 atacagtaat aaaattttta tgctttttgt acgctgaatt aataatttta tatagatttg
     1801 cagggatgag aaaaggaaat ttatataact ggtggtggtg cagcttctga actgatgctg
     1861 tgaatcacgc tgttatataa atacaaaaga tataattgaa aagcacgctg atcacagcgt
     1921 gctttttgtt ttaaaaaacc tttataatac ttaattaata atcgaagaaa tgaaaaaaaa
     1981 cacaaagatt cttttaagcg aaagagagat gccccggcaa tggtataaca ttgtggcaga
     2041 catgccaaac aagccggcgc ccccgctaaa tccggcaact cgcgaaccca ttgatcccgc
     2101 agctctggca gcagtgtttc cgatggagct tatcaggcag gaaatgtcag cggaaaggta
     2161 tattgacata cccgacgagg tcctgaacct ttataaaatc taccgccctt cccctcttca
     2221 ccgggctctt aatcttgaga aagccctcgg gacaaaaagc aggatctatt acaagtatga
     2281 gggtggcaac tattccggtt cgcacaaggc caacaccgca attgcccagg catattacaa
     2341 caagaaggaa ggtataagga gaatcactac cgaaaccgga gcgggccagt ggggaagtgc
     2401 aatgagcttt gcatgcaacc atttcggtct tgagctgctg gtcttcatgg tcagtgtaag
     2461 ttttcaccag aaacccggcc ggaaaatcat gatgaacacc tataatgcca aggtggttcc
     2521 ttcgccctca accatgaccg ccgttgggag gcagatcctt gaaaaggatc cggattcccc
     2581 gggaagcctg ggaatagcca tctctgaagc catggaaata gccgttcagg atccttatac
     2641 aaaatattcc ctcggcagtg tactgaacca tgtttcactg catcagacca taattggcca
     2701 ggaagcaatg attcagatgg aaaaggccgg tgactacccc gatgtggtaa taggatgctt
     2761 cggaggcgga tctaatttct cgggcatcgc cttccccttt atccgtgaaa atattgtcag
     2821 tggcagaaag accagattcg ttgcagtgga accttcttca tgcccgaaac tgaccaaggg
     2881 aagattctta tacgatttcg gtgattctgc cggaatgacc ccgcttcttc caatgtacac
     2941 cctgggacat aattttattc cggaaaaaat acatgccggg ggcctgcgtt atcatggagc
     3001 aggagttctt gtaagccagc ttcttaaaga cggatttgtt gaggcaaagg ccaaaatgca
     3061 gagggaatgc ttcgaagcag gagtattttt tgccagaaat gaaggcattc tccccgctcc
     3121 ggaatccaac tatgcgattg cagccgcact cgatgaagcc agacaatccg acatcgaagg
     3181 gacaagcagg gtaattctgt tcaatctcag cggacacggc cacatggatg tctcatccta
     3241 ccagaaattc ttcgaggata atcttcccga ccatgaaatg acacaggaag aagttgataa
     3301 atttattgcc gcatccggtc tggagggctg acttttacaa gaaacccccg gaggaaatcc
     3361 gggggttaaa tatcatttaa aaatcggaac aggatagcgg atacaggagg tactgcgtgt
     3421 ttctggaaac agtattcctg tattcttcct tatcgcgcat ccttatccag tcgggatcgt
     3481 ttctgaattc ctcccatttt tcatcgtggg tcttcatatt ctgaaacgtg gtcatgtaca
     3541 tcaggttggg catgtggctc cctgccagga cctggccgta aaagacagca ttgaatccca
     3601 gtttttcgaa aagggcgatt tcctcattgt tgaacatgtc gatctttttt ccggccttgt
     3661 attccgtggc actctcatag cttctcagct catacaccct ttctgaccgg tcagtggcga
     3721 atgaaggggt gctgaaacgg ggcatcccgg cgaaggcttt aagcaaaatg ctttcatacc
     3781 tctgaaaagg cggattatca tgcggcgcat ttaaaaagtc actgcctctt ttctgataat
     3841 cagcatcttt ttcaagcacg accttcatgc tgaaatattt gtccgcagtt tcatagggaa
     3901 tgaacacata aatatatttc ccggaagcag cagtgtcgga ttcaagtggc ttgaacaccc
     3961 cgactccttc tatgccggat ctgcgaagga cgggcttgag agctccggaa atatatcttt
     4021 cggttctctc tgcctggccg ggtccggaga catcataaat ttttatctca tagagcatct
     4081 gtggtatttg ttccctgcag ccgcctgccg gaaagagcag gactgccaca aataccatgg
     4141 aaaacaaggt tctgatggcc tttgtcataa acacaagcat ttaaaatttg caatcagtca
     4201 tcctgcatct gtactaatac aatgccgaga attccctgaa gatactgttg gcctgtccgg
     4261 cggtaatttc cttgcccgac tgtatgtaaa acctgtgacg cagtgttaca gattcacctt
     4321 ttccgagggt aaggactatc ttttcgtcat ccttgttata ggcctgggaa cccaggttat
     4381 tggttgaaaa gagaccatag ccgcgcgcat gtgagtgccc cggataacca atgtttttgg
     4441 gatgatccca gaaaccgaaa gatgtcagta cattattttt cgttcctgaa agaattaccc
     4501 agtcattcct tgtactccat acatcatctc cgcgtttccc ggaacttgat gtatacattc
     4561 ccgttacgcc ttcgttatca gttgccctga cagtggtcgg atttccctgc gaatcagtat
     4621 agactgccgg ctgattcgtg ggcatttcaa aggccctgtc aacccttatg gcaaacattc
     4681 cttccttgtt gtcggtaatg gttaccggac cttcagcagc cgtcagggtg gagatatggt
     4741 caatggtata agaaaccttg gtacccgaga aaatatactg tgtgttttcc gtaagcagag
     4801 tatttccctt gttatccttc cagtcggata tcacctcaag aacggctttt ttcccgtttt
     4861 tgaccttcac gatttccctg actgcaatat gtccgtaagc atcttttttc tccgccggaa
     4921 ttgccgagga gttattccag aaatccagtc cgttcacatc cccgtgattg aaccatattc
     4981 cgatatgatg cggatgatca accctttccc tgggccgggg ttccagggga aacccccttg
     5041 taaccaccga accgtccggc gcattgacag gccagagata cggtttttcc atattggacg
     5101 gatactgata ggaagtaaac agctttcccc ctatcatcac atccaccttc tggccgcctt
     5161 tgata
//
LOCUS       DAOSPX010000232         5164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14667_length_5164_cov_3.056371, whole genome shotgun sequence.
ACCESSION   DAOSPX010000232 DAOSPX010000000
VERSION     DAOSPX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14667_length_5164_cov_3.056371"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLZ75_10520"
     CDS             complement(<1..261)
                     /locus_tag="PLZ75_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOS72866.1"
                     /translation="MKSISDILLQRKTIRKYSDRPLDGNLLHDILEKGCRASTTGNMQ
                     VYSVIITRDADMKRNLLPLHFNQPMVTEAPVLLTFCADFNRFS"
     gene            492..1484
                     /locus_tag="PLZ75_10525"
     CDS             492..1484
                     /locus_tag="PLZ75_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS72867.1"
                     /translation="MSRPVTLFTGQWADLPLETVCEKASSFGYDGLELACWGDHFEVN
                     KADDAYCSAKRKLLEKYNLKLFAISNHLVGQAVADRIDERHKSILPPYVWGDGNQDGV
                     RKRAAEEMVRTAEAAKRLGISVVNGFTGSPVWHLAYSFPPNVPGMIENGFREFADLWK
                     PVLDEFQKLGIRFGLEVHPTEIAFDIASARKALEVLNNHPAFGFNYDPSHFGYQGVDY
                     VGFIYTFSERIFHVHMKDAGWSDIPCEAGVFGGHTEFGARGRYWDFRSPGRGNIDFEE
                     IIRALNRIGYNGPLSIEWEDSGMDREFGAREACDFVRSIDFPPSDIAFDSAFEK"
     gene            1635..2768
                     /locus_tag="PLZ75_10530"
     CDS             1635..2768
                     /locus_tag="PLZ75_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72868.1"
                     /translation="MKKIKMGMIGGGTGAFIGDAHRRAARICNDYDLVGGVFSSNPER
                     NRESAAREGIAPDRCYDSVDAMIAGELAKPEDERIRLVSIVTPNSLHFSQAKAFLSNG
                     FHLVCEKPMTMTVEEAVELEQLVEKTGLTFALAHTYTGYPMVRQMRQLISKGVLGTIQ
                     RIDAQYYQGWINAIIHGAESRITGVWRLDPKNAGVSSCMGDIGVHAFNLIEYTTGLEI
                     KEILSDLNKVKEDVELDLDGTVLLRFNNKLKGVIRASQVCGGLENDIVISVYGSRASL
                     RWEQENPNYLYMFSDTEPVRVYKPAHAYNEKMAEESHTMPAGHPEGIYDALANIYRGA
                     AKSIRGEEYTPGEFPTVHEGVRGMKFIHAVVSSNAQGNIWTKI"
     gene            complement(2883..3152)
                     /locus_tag="PLZ75_10535"
     CDS             complement(2883..3152)
                     /locus_tag="PLZ75_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOS72869.1"
                     /translation="MKKALLTFSLVTLLVFGGLAPLFSQEQPRPQKDTVNIDTDAKPT
                     TYYDIEDENLPKQVKSKGSGATVAIIAGAVVVAGAVAYFLLRKKK"
     gene            complement(3170..>5164)
                     /locus_tag="PLZ75_10540"
     CDS             complement(3170..>5164)
                     /locus_tag="PLZ75_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS72870.1"
                     /translation="HLKYYGQAFDNAVTFLIKWFGIEDVYSFRHLMSSLSGWLAVFIT
                     ALFGIWLSGYRTGLLILLLYAVSPTFTGHSMNNLKDIPFALAYIAGIFFTLKLLEDEK
                     PVSWKNITAVIISTGFAIGIRPVGILLICYIFLFWFILRLPHLYRITVKKTGNKGTGY
                     EGIAEDGALNQGVLYEEAVSGEVRNREGKFRGIRDLLAELGILVFISGAGFFLGSLLW
                     PYALQSPVRNILGSYHFMAHFPGTFRQIFEGSNEWSDFMPWYFIPKSMLITIPLIVLA
                     GAVLFLVFAGRAARPGKLVLYLIIIFTFLFPMLFVIVKQSNLYSSWRQFLFLYPPLIL
                     IAASGLNYVYEAVPGKYSGWPVTAVIILMSIHPAVYMIRNHPYEYIYFNQFTGGLKGA
                     YGNYETDYYYVNQTEASEWLIAYLTEKGIDSAVVGATYPVKWQFRKFPGIKTFYFRHE
                     ERSQYNWDYAIITNRYIHPFQLKNGIWPPPEPLHTIYADGIPIGIILERKNRDDFYGY
                     RALEENRPADAVRHYEAALQFSDRDEMIFYNFARALYNEGHHERADSVLKKGLEINPF
                     CEPILMYLGNIAKYRGNTDEALKYYERLISYNRKYFQAYVEMAGLYSGNDNPRARELL
                     RSCLRLNPRYRPAIVALADTYREEDPERAKKYDEMAETVK"
BASE COUNT         1422 a         1208 c         1211 g         1323 t
ORIGIN      
        1 gctaaaccgg ttgaaatcgg cacaaaaagt cagtaataca ggagcctcag taaccattgg
       61 ctgattgaag tgcaatggca gaagattcct tttcatgtct gcatccctcg taataatcac
      121 actgtaaacc tgcatgttcc cggtggttga tgccctgcag cccttttcca gaatatcatg
      181 aagaagatta ccgtcaagag gcctgtccga gtatttgcgt atggtcttcc tttgaagcag
      241 aatatctgat atggatttca tttattaact attaaggatt catcacaaag aaaagaaatt
      301 cttcagaatg cttacggctc cttgaacagg attttcttat attagcatga ttcaaatgat
      361 aaccctgatg aaacccctgt ttcccatttc ctgcatcccc cgtacggccg tcactgcctt
      421 atatcagtgc aggaatggcc atgagccctg ccggaaacaa cattataaaa tccatataaa
      481 acattaatac tatgagcaga ccagtaacat tattcacggg ccagtgggca gatcttcccc
      541 tggaaacagt atgcgagaaa gccagttcat tcggttatga cggactggaa ctggcatgct
      601 ggggagacca ttttgaggta aacaaggcag atgatgccta ttgcagtgca aagcgcaagc
      661 ttctcgagaa atataacctg aagctttttg ccatatcaaa ccatcttgtc ggtcaggcag
      721 ttgcagaccg gattgatgaa cgtcacaaaa gcatactacc gccgtacgta tggggagacg
      781 gcaaccagga tggtgtaagg aagcgtgcgg cagaagagat ggtaagaacg gctgaggccg
      841 cgaaacgcct tggcatatca gtcgttaacg gctttaccgg aagtcctgtg tggcacctgg
      901 catattcctt cccccccaat gtccccggaa tgattgaaaa cggtttcagg gaatttgcag
      961 atctgtggaa accggttctg gatgaatttc agaagctcgg catccgcttt ggcctcgagg
     1021 tgcatccgac tgagattgca tttgatattg catcagccag aaaggcactg gaagtcctca
     1081 ataatcaccc tgccttcggg ttcaattatg acccttccca tttcggatac cagggagttg
     1141 attatgtagg attcatttat actttcagcg aacgcatctt tcatgtccac atgaaggatg
     1201 ccggatggtc cgacataccc tgtgaggcag gtgttttcgg agggcatacc gaattcggcg
     1261 ccaggggccg ttactgggat ttcagaagcc ccggccgcgg caatattgat tttgaggaga
     1321 tcatccgggc cctgaacagg ataggctaca acggccctct gtcaattgaa tgggaagaca
     1381 gcggaatgga ccgtgaattc ggagcaagag aagcgtgcga ctttgtcagg tccattgact
     1441 ttccgccttc ggatatcgcc ttcgattcag cctttgagaa gtgatcattc tccggaaagg
     1501 caaatcagct ttctcagaat gagatgatgg gtttttctct gaaaaggctg tatccatgta
     1561 cacaactttg taaaaatctt tatcccgctc ttccgggatg aaggtttctt ccttaaataa
     1621 aaaacttcaa aatgatgaaa aaaatcaaaa tgggtatgat aggcggcggc acaggagcat
     1681 ttataggaga tgctcatcgc cgggccgcaa ggatctgtaa cgattatgac ctcgtcggag
     1741 gtgttttcag cagcaatcct gaaaggaaca gggaatctgc tgccagggaa ggaatagccc
     1801 cggaccgctg ttatgacagt gttgatgcca tgatagcagg cgaacttgca aaacctgagg
     1861 acgaaaggat caggcttgtt tcaattgtca ccccgaattc tcttcacttc tcacaggcaa
     1921 aggcatttct ttccaatggt ttccacctgg tctgtgaaaa gcccatgacc atgactgtgg
     1981 aagaagccgt tgagctggaa cagctagttg aaaaaaccgg actcaccttt gcgcttgccc
     2041 atacctatac cgggtatccg atggtaaggc agatgaggca actgatttca aaaggtgtgc
     2101 tgggaaccat ccagagaata gatgcccagt attaccaggg atggatcaat gccatcatcc
     2161 atggtgctga aagcagaatc acaggcgtgt ggaggctgga tccgaaaaat gccggcgtaa
     2221 gcagctgtat gggagatatc ggcgttcatg cattcaatct gatagaatat actacaggac
     2281 tggaaataaa agagatattg tctgatctga acaaggttaa ggaagatgtg gaactggatc
     2341 tcgacggaac cgtgctgctg cgtttcaaca ataagctgaa gggcgttatc cgtgcaagtc
     2401 aggtttgcgg cggactcgaa aacgatatcg tcatttctgt ttacggatca agggccagtc
     2461 tgagatggga acaggaaaac ccgaactacc tctacatgtt cagtgatacc gaaccggtaa
     2521 gggtatacaa gcctgcgcat gcgtacaacg aaaaaatggc cgaggaaagc cacacaatgc
     2581 ctgcagggca tcccgaagga atttatgatg ctctcgcgaa catatacagg ggagcggcaa
     2641 aatcgatccg cggagaagaa tacactccgg gtgaattccc gacagtgcac gaaggcgtca
     2701 ggggcatgaa attcattcat gccgtagtca gttccaacgc acagggtaat atctggacga
     2761 agatttagat tctgtacaag gatcctgcaa accgcagaaa atcccggagc agacagaggc
     2821 cgcaggatat ctgcggcctg tcttcagccg ggctccggtc tgaaccggat tactgcagct
     2881 ttttatttct tctttctcag caggaagtat gcaacagctc cggcaacaac cactgctccg
     2941 gcaattattg caactgtagc accgcttccc ttgcttttta cctgctttgg caggttctca
     3001 tcctcaatat cataataggt agtgggcttt gcatccgtgt caatatttac ggtatccttc
     3061 tgtggccggg gctgttcctg ggaaaacagg ggggccaggc cgccaaatac caataaggtt
     3121 acgagagaga atgttaataa tgcttttttc ataacagata taatttaaat tatttaacag
     3181 tttcagccat ttcatcgtat ttttttgctc tttcaggatc ttcttccctg taagtatctg
     3241 ccagggcgac aatggcaggc ctgtaccggg gatttaaccg caggcagctc ctcaacagct
     3301 ccctggccct tggattgtca ttgcctgaat acaatcccgc catttcaaca taggcctgaa
     3361 aatattttct gttatagctt atcagccttt cataatactt cagggcctca tccgtatttc
     3421 cgcgatactt tgcgatattc ccgaggtaca tcagtatcgg ttcacagaaa ggattgattt
     3481 ccagtccttt cttcaatacc gaatctgccc tttcatgatg tccctcattg tacaaagctc
     3541 ttgcaaaatt ataaaaaatc atctcatccc tgtcactgaa ctgcagggct gcttcataat
     3601 gcctgacagc atctgccgga cgattctcct ctagagccct gtatccgtaa aaatcatccc
     3661 tgtttttccg ttcaagaata atgccgatag gaataccgtc ggcatatatt gtatgcagcg
     3721 gttccggcgg cggccatatt ccgtttttca gctgaaaggg atggatatag cggttggtta
     3781 taattgcata atcccagttg tactggcttc tttcctcatg cctgaaataa aaggttttta
     3841 ttcccgggaa tttccggaac tgccatttaa caggatatgt tgccccgacg actgccgaat
     3901 caataccttt ttctgtcaga tatgcaataa gccattcaga agcctccgtc tggttcacgt
     3961 agtaataatc cgtctcataa ttgccgtaag ctcctttcag tccgccggta aactgattga
     4021 aataaatgta ttcataggga tggttcctta tcatatatac tgccgggtga atactcatca
     4081 gaatgatcac tgcggtgacc ggccatcctg aatattttcc cggaacggct tcataaacat
     4141 aattcagtcc ggaagccgct atcagtatca gcggagggta gaggaaaaga aactgtctcc
     4201 aggagctgta gagatttgat tgctttacaa tcacgaaaag catcggaaac agaaaagtaa
     4261 aaatgattat cagataaaga accagcttcc ccggcctggc tgcccttccc gcaaaaacca
     4321 ggaaaagcac tgctcctgcc agcacaatca gcgggattgt tatcagcatt gatttgggga
     4381 tgaaatacca tggcatgaag tcggaccatt cattgcttcc ttcaaagatc tgccggaaag
     4441 tgccggggaa atgtgccatg aaatgatatg agccgagtat gtttcttaca ggagactgca
     4501 gggcataggg ccacagtaat gagcccagga aaaatcctgc tccggaaatg aaaaccagaa
     4561 tccccagttc cgctaacaga tcccttatcc ctctgaactt cccttctctg ttacggactt
     4621 ccccggatac ggcctcttca tacaatactc cctggttcag ggccccatct tctgcaattc
     4681 cttcatatcc ggttccctta ttcccggtct ttttcaccgt aatcctgtac aggtgaggca
     4741 gccgcaaaat gaaccagaaa aggaatatat agcagatcag gagtattcct accggcctga
     4801 ttcctattgc aaatccggta ctgattatta ctgccgtgat atttttccac gaaacaggct
     4861 tttcatcctc cagcagtttc agggtgaaaa agataccggc tatatatgca agggcaaagg
     4921 gtatatcctt gagattattc atggaatggc ccgtgaatgt cggggatacg gcataaagaa
     4981 gcaggatgag aagacccgta cggtaaccgg agagccatat tccgaagaga gcggttataa
     5041 aaacggcaag ccatcccgac agtgagctca taaggtgtct gaagctgtaa acatcttcaa
     5101 taccgaacca ttttatcaga aatgttaccg cattgtcaaa ggcctggccg taatatttca
     5161 ggtg
//
LOCUS       DAOSPX010000233         5146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14730_length_5146_cov_4.476134, whole genome shotgun sequence.
ACCESSION   DAOSPX010000233 DAOSPX010000000
VERSION     DAOSPX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14730_length_5146_cov_4.476134"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLZ75_10545"
     CDS             complement(<1..647)
                     /locus_tag="PLZ75_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HOS72871.1"
                     /translation="MRDLQHIKQRFGIIGNYEGLNRALDIAVQVAPTDLSVLISGESG
                     TGKEVFPQAIHSYSTRKHAPYIAVNCGAIPEGTIDSELFGHEKGAFTGATDSRKGYFE
                     FADGGTIFLDEVAELPLSTQVRLLRVLETGEFIRVGSSKVQKTNVRVIAASNVDVTRE
                     INQGRFREDLFYRLNTVPIRVPALRERGEDIILLFRKFAVDFAEKYRMPAIKADA"
     gene            723..1496
                     /gene="kdsB"
                     /locus_tag="PLZ75_10550"
     CDS             723..1496
                     /gene="kdsB"
                     /locus_tag="PLZ75_10550"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOS72872.1"
                     /translation="MKSISGLNFAVIIPARYASTRFPGKALAMIGGKYMVQRVYEQAR
                     KSVDKVYIATDDQRIFYAAESFGADAVFTSADHTSGTDRCAEAVKKIMAGTGEKIDVV
                     INVQGDEPFIKPEQIDLLKSCFSDKNVTLATLVRKTAQGEDIFNPGRPKVILDVNKDA
                     IYFSRAAIPHIRDHQSAQWSSMHSYYMHIGLYAYSSDILEKITRLPQSSLEKAESLEQ
                     NRWIENGYKIRCAETVWESFGIDTPEDLDKALSNFKRYI"
     gene            1548..1919
                     /locus_tag="PLZ75_10555"
     CDS             1548..1919
                     /locus_tag="PLZ75_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS72873.1"
                     /translation="MKKTLLFVMFVFFSLSLFSQKDTAGIASARLKQDLPARAATATV
                     SIYPVPVRENYFNIKTDQDISSIKITNIIGQVIYKVQYNGPQMLSTIYLDNPRRGMYL
                     VSILFSDGSRMVKKIMIEPSE"
     gene            complement(1957..2943)
                     /locus_tag="PLZ75_10560"
     CDS             complement(1957..2943)
                     /locus_tag="PLZ75_10560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOS72874.1"
                     /translation="MELRTIFKIEESAEKITYNDPVMFIGSCFAGGMGKQMEAGKMPV
                     MVNPSGTVYNPVSVANTLDTITQRRSYTVDDLYNHNGTWLSFNHYTDFSSNDPDIVTG
                     RINKAGEEAFRFLSGASFLFITFGTARVYRWKQSGRIVSNCHKIPATYFTNELLKVSD
                     IVTLWEAVLDRLKTLFPGLRVIFTISPVRHWKDGAHGNQVSKSVLFLAIEELLGHSGS
                     PGYFPAYELLMDDLRDYRFYDDDMLNPSSFAIRYIWQRFSDCYLDAATAELRNEALKI
                     RKAMGHRLGDTPHDRKKEFAKGMLEKISVMENKFRRIDLREERSYFQGIADE"
     gene            complement(2986..3807)
                     /locus_tag="PLZ75_10565"
     CDS             complement(2986..3807)
                     /locus_tag="PLZ75_10565"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOS72875.1"
                     /translation="MKYSIKLRNGQILRGLIVSPGENARANIVFVHGLGEHIGRYVPW
                     AELFRNAGIGFTGVDLPGHGSSGGKRGHIRSYDLTDEMIDLLISHTGSAFTGVPVFLY
                     GHSLGGNIVLDYLLRKTPGIAGAVVTSPWLILRAQPGKSKLVLASAMKYVLPGLTQAS
                     GLNPEHLSRDRDVVERYLADPLVHERISVSLFHNAAGAAKNSLSASSSLKVPLLLMHG
                     SDDQITSPEGSREFASGNNLTEIKIWDDGYHELHNEPFREEVFSFITDWINRMSA"
     gene            4229..4735
                     /locus_tag="PLZ75_10570"
     CDS             4229..4735
                     /locus_tag="PLZ75_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOS72876.1"
                     /translation="MTTHEFNNNLIEMKSNLQRFAMSLTYDRETALDLVQDTYLKAIT
                     NKDKFVDYSNLKAWVFTIMKNTFINNYRRSVKENTIIDGTKDLFYLNQPNDKGFISPE
                     SNYSEAEIEKAIDSLEDEFRIPFRMHISGFKYKEIADELGLKIGTVKSRIFFTRQKLM
                     LMLKDYNG"
     gene            4862..>5146
                     /locus_tag="PLZ75_10575"
     CDS             4862..>5146
                     /locus_tag="PLZ75_10575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOS72877.1"
                     /translation="MQKIIKITTRHRNALYDITSAVEEAVRESGVKTGHASVYAKGST
                     CAIMIQENWDESVQNDVITLLDRIIPEGIWQHDLQDNNGAAHLKAGLIGPS"
BASE COUNT         1489 a         1184 c         1079 g         1394 t
ORIGIN      
        1 tcagcatcgg ccttaatggc aggcattctg tatttttcgg caaagtcaac agcaaacttc
       61 ctgaaaagca ggattatgtc ttcaccacgt tcccgcagtg ccggcactct gatcggaaca
      121 gtattgagcc tgtagaaaag atcctccctg aatcttccct ggtttatctc ccttgtgaca
      181 tcaacattgc tggctgcaat aacccttaca tttgttttct gtacctttga tgatcctacc
      241 ctgataaatt caccagtctc aagcactctc agaagtctta cctgtgttga caggggaagt
      301 tcggcaactt cgtccagaaa gattgttcct ccgtcagcaa attcaaaata gcccttccgg
      361 ctgtctgttg cacctgtaaa agcccccttt tcatgtccga acagttcgga atcaatggtg
      421 ccttcaggta tggctccgca gttaacggct atataggggg cgtgcttcct ggtgctgtaa
      481 ctgtgaatcg cctgtggaaa tacctccttt cccgttccgc tttcaccgct gataagaaca
      541 gaaagatcag ttggggcaac ctgaacggca atgtccagag cccggttaag tccttcataa
      601 ttgccgatta tcccgaatcg ctgctttatg tgctgtaggt ccctcattaa attccttgaa
      661 acagatgcga atttaagata tttttaaaac aatgggaaat ccatttttcg taacttggca
      721 gcatgaaaag catatcagga cttaattttg cagtcattat tcctgcacgt tatgcatcaa
      781 cgcgctttcc cggaaaggcg cttgcaatga taggtggcaa atacatggtc cagagagtct
      841 atgagcaggc ccgcaaatca gtggataaag tgtatattgc aacggatgac cagagaatat
      901 tttatgcagc agaaagcttc ggcgccgatg ccgtattcac ctcggcggat cacacaagcg
      961 ggacagaccg ctgtgccgaa gcagtaaaaa agatcatggc cggaaccggg gaaaagattg
     1021 atgtcgtcat caatgtgcag ggcgatgaac catttataaa acccgaacag atagatctgc
     1081 tgaaatcatg tttttctgac aaaaatgtca ctttagccac tcttgtacgt aaaacggcgc
     1141 agggggaaga tatttttaat cccggccggc caaaagtcat cctggacgtg aacaaagatg
     1201 ccatatactt cagcagggct gcaattcccc atatcaggga tcatcagtcc gcgcaatgga
     1261 gcagcatgca cagctattat atgcatatcg gactgtatgc atacagcagt gatatactgg
     1321 aaaaaatcac ccggctgcct cagagctcac tggaaaaagc ggaatcactt gaacaaaaca
     1381 gatggataga aaacggctat aaaataagat gcgcagagac cgtctgggag agtttcggca
     1441 tagatacacc tgaagatctg gataaggcat taagcaattt taaacgttat atttaatttt
     1501 ttattatctt taacacaaat tttcatgcaa tacaccaaaa caacaatatg aagaaaactc
     1561 tactctttgt aatgtttgta tttttcagcc tcagtctttt ttcccagaaa gacactgccg
     1621 gtattgcatc agcccgcctg aaacaggatc tcccggccag ggccgccact gccactgtaa
     1681 gtatttaccc ggtccctgtc agggagaatt atttcaatat aaaaaccgat caggatattt
     1741 cttcaataaa aataaccaac atcataggtc aggtcatcta caaggttcag tataacggtc
     1801 cccagatgct ctcaacaata tacctggaca atccgcgccg gggaatgtat cttgtttcaa
     1861 tactgttcag cgacggatcc aggatggtaa aaaagataat gattgagcct tccgaatagg
     1921 actctgacca gggcagcaac tcctgcctgt gtatttttat tcatctgcaa ttccctgaaa
     1981 ataagatctt tcttccctca gatctattct acggaattta ttttccatca cacttatctt
     2041 ttcaagcatt ccctttgcaa actccttctt cctgtcgtgg ggagtatcgc ccagcctgtg
     2101 ccccattgct tttcttatct tcaaagcttc atttctgagc tctgccgtag ctgcatccag
     2161 gtagcagtca gaaaacctct gccagatata tctgattgcg aatgatgatg ggttcagcat
     2221 atcatcatca taaaacctgt aatccctgag atcatccatc agcagttcgt aggcgggaaa
     2281 atagcccggc gagccggaat gcccgagcaa ttcctcgatg gcaagaaaaa gtactgattt
     2341 gctcacctgg tttccatggg caccgtcttt ccagtgacgc acaggactga tggtgaatat
     2401 caccctgagc ccgggaaaga gagtcttcag cctgtcaaga acagcttccc acagggtaac
     2461 aatgtcagag accttaagca gttcattcgt aaaatacgtt gccggtattt tatggcagtt
     2521 ggatactatc cggccggatt gtttccagcg gtatacccgt gccgttccga aggtgataaa
     2581 aagaaaggag gccccggaaa gaaatctgaa agcttcttcg cctgccttgt ttatcctgcc
     2641 ggtcacaata tccggatcat ttgaggaaaa atcagtgtaa tggttgaagc tcagccaggt
     2701 gccgttgtga ttgtaaaggt catccacagt atagctgcgc ctttgtgtta ttgtatcaag
     2761 ggtattggcc acggataccg gattatatac cgtccccgaa ggattgacca tcaccggcat
     2821 tttccccgct tccatctgtt ttcccattcc gccggcaaaa caggacccta taaacatgac
     2881 gggatcatta taagttatct tttcagcaga ttcctctatt ttaaagatcg ttctgagttc
     2941 catacaattc cgtgattttc agaaatgact gctcaccagg ccctgtcatg cagacatcct
     3001 gtttatccag tccgtgataa acgaaaagac ctcttccctg aatggttcat tgtgaagttc
     3061 atgatatcca tcgtcccata ttttaatttc cgtaagattg tttcctgagg caaattccct
     3121 gcttccttcg ggagaagtta tctgatcatc actgccatgc atgagaagca atggaacttt
     3181 cagggatgat gaagcggaaa gggaattttt tgccgctcct gccgcattgt ggaaaagtga
     3241 tactgaaatc ctttcatgaa caagagggtc cgccagatag cgttcaacaa catcccggtc
     3301 gcgggataaa tgttcagggt taaggcctga ggcctgagtc agccccggaa gaacatactt
     3361 cattgctgat gccagtacca gttttgattt tcccggctgt gccctgagta tcagccatgg
     3421 cgaggtaaca accgctcctg ctatcccggg agtttttctc agcagataat ccaggacaat
     3481 attccctcca agactgtggc cgtacagaaa aacaggaaca ccggtaaaag ccgatcccgt
     3541 gtggctgatg agcagatcaa tcatctcatc cgtgagatca taacttctga tatgacccct
     3601 ctttccgccg gaagaaccgt gccccggcag atcaactccc gtaaatccga ttccggcatt
     3661 tctgaataat tccgcccatg ggacatatct gcctatatgc tctcccagac cgtgaacaaa
     3721 aacaatattt gcccttgcat tttcaccggg actgacgata agtccccgca aaatctgacc
     3781 gttccttaat ttaatgctgt atttcatttc tgcgttccgt taaattattg cctgaaagca
     3841 ttcatccgga caatatggca acccgtgcat cccatgaacg gatgctcata aaatattcat
     3901 aaatttaata aagtgaaatc aatttccccc ggaaagtgta actattcttc tccgttcagg
     3961 tgaaattctc catcctcatt aagagctccg gccggatgtc ctgtccgcaa agtctgaacc
     4021 ggactccacc ctgacatgat gaaaatattt tttacatttt attgtaattt ataaaaagac
     4081 ataaaccacc ccattataaa attaattatc tgtcatttat tcagaatcac aatttagaat
     4141 aggaccggat ttaacagaat ttaggatatt aagatttgca tatctttttt tattgattat
     4201 ttttgtagca tcaataaaca atgaatccat gacaacgcac gaattcaata ataacttaat
     4261 agaaatgaag tcaaatctcc agagatttgc aatgagcctc acatatgacc gtgaaactgc
     4321 gcttgatctg gtgcaggaca catatctcaa agctattacc aacaaggata agtttgttga
     4381 ttattcaaat ctcaaggcct gggtttttac catcatgaag aatactttca tcaacaatta
     4441 caggcggagc gtgaaagaga atacaataat tgacggaaca aaggatctct tctatttaaa
     4501 ccagccgaat gacaagggat ttatctcacc cgagtcgaat tattcagaag cggagataga
     4561 gaaagcaata gattcacttg aagatgagtt caggattccc ttcagaatgc acataagcgg
     4621 ttttaaatac aaggagattg ctgatgagct cggcctgaag ataggaacgg taaaaagcag
     4681 gattttcttt acaaggcaga aacttatgct gatgctcaag gattacaacg gatgattatt
     4741 ataaaaagat ttgtaaggtt cgaagattgg tcaggttttt gatcaggttg attaggttaa
     4801 gtcaggaaaa aaggctgaag ggtttcagcc tttttttatt attattgtaa aaaataacct
     4861 gatgcaaaag ataataaaga tcaccacccg ccatcggaat gcgctttatg atataacatc
     4921 cgccgtggag gaagcggtca gggaaagcgg agtgaaaaca ggccatgcat cagtatatgc
     4981 caagggctcc acatgtgcaa taatgattca ggaaaactgg gatgaatctg tacagaatga
     5041 tgtcattacc ttgcttgaca ggattatacc ggagggtata tggcagcatg atctccagga
     5101 caacaacggg gccgcccatc ttaaagccgg cctgatcggc ccttcc
//
LOCUS       DAOSPX010000234         5131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14798_length_5131_cov_4.462963, whole genome shotgun sequence.
ACCESSION   DAOSPX010000234 DAOSPX010000000
VERSION     DAOSPX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14798_length_5131_cov_4.462963"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLZ75_10580"
     CDS             complement(<1..385)
                     /locus_tag="PLZ75_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72878.1"
                     /translation="MKNSGDIMEEFIDPAAGGGRNSEEDNNHVPELPGEDFLRLSSSR
                     RDFLKAFGFSFASAALIAGCKIPVRKAVPYVNQPPEFSPGESVYYATSFYDGHEYSDI
                     LVKTRDGRPVKLEGNPLSGFNGEGTT"
     gene            complement(414..1622)
                     /locus_tag="PLZ75_10585"
     CDS             complement(414..1622)
                     /locus_tag="PLZ75_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOS72879.1"
                     /translation="MRKSCSRKYSPGLSALTFSSLVFIFSAGFSHPASADLPPDAPGK
                     QELQSEDSVKSISGSVIQASEELIRGQRLFYGLVYPDNKAMNCAACHNTAVTDTLNWN
                     PDAVEISGKYKNKSVDDLTEILLSPTGKKMAQVHRDFRFTPEEIMLIKGYMDELAGTG
                     LTQRKPPVTNLVLFITAFFLFFVSAVDLIITKKIKKRWINPVVLSIATVYITWSLVVN
                     AIDLGRSPGYSPDQPVKFSHQIHAEQNGTDCIYCHNYAPYSKSAGIPAQNICMNCHLL
                     VRSGTRSGMFEIAKVTGSYEEKRPIEWIRVHNVPDHVFFSHAQHVGAGGIDCAECHGD
                     VAKMVRIRQVSDLSMGWCIKCHRTRKVNFSGNNFYSQYHELSDKLRKGVTDSITPERV
                     GGTECMKCHY"
     gene            complement(1680..2354)
                     /gene="narI"
                     /locus_tag="PLZ75_10590"
     CDS             complement(1680..2354)
                     /gene="narI"
                     /locus_tag="PLZ75_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOS72880.1"
                     /translation="MNGFFFFVLLPYLALFSLLPASIYKYRVHGFRVSSLSSQLLENK
                     LLFFGSRPFHWGIILLFFGHLIAFLIPASVLAWNEKPVRLYILEVAALSFGFMTLIGF
                     LILVYRRLRVKRIRLVTSRMDVFVFLILAVQIVTGLYTALFFRWGSSWFATVLTPYLK
                     SVFAFRPDVAAVTALPVMVKIHIASAFVLVGMIPYTRFMHFLVYPFVYFWRRYQIVIW
                     NKRESA"
     gene            complement(2351..2944)
                     /locus_tag="PLZ75_10595"
     CDS             complement(2351..2944)
                     /locus_tag="PLZ75_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72881.1"
                     /translation="MKDLTHYGLLAEMFRYPTDDLKNYPEKWNDIVSRYDASLMSRLD
                     RFTAHVRGKPLAFQQEYYVGTFDVQALCFLDVGYVLYGEDYRRGIFLVNIKKEQIKAK
                     NDCGSELPDHLPNILTLLPRMADPELAEELICSMLIPAIHRMIAEFRDMSNLYKGLLE
                     ILAGIMEADFPFSTFERFTFGGRENEIIINKNQTGWK"
     gene            complement(2946..4436)
                     /gene="narH"
                     /locus_tag="PLZ75_10600"
     CDS             complement(2946..4436)
                     /gene="narH"
                     /locus_tag="PLZ75_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HOS72882.1"
                     /translation="MNIKSQISIVFHLDKCIGCHTCSIACKNAWTDRKGAEYMWWNNV
                     ETKPGTGYPRRWEEQDLYRGGWEKDGSRVRLKGAGKIKGTGVIFQNPNLPALDNYYEP
                     WTYRYEKLLDAPAGDDQPTAEPVSLITGDRITPKSGPNWDDDLGGSPIYARNDTNLSD
                     LTETEKEALFQLERMVMFYLPRICNHCLNPACVAACPSGAIYKRGEDGIVLINQKKCR
                     AWRLCVTACPYKKVYFNWNTGKSEKCILCYPRIETMQIPACMHSCVGRIRYLGVMLYD
                     ADRIREAASAEDRDIVGSQLDLFLDPNDPVVISQAKKSGIPDSTMEAARKSPVWKYVK
                     EWGLALPLHPEFRTLPGLFYALPLLPAMATMENNFYSSTGSSLWNNVDDSRLPMKYLA
                     NLFSAGDTELVRNILKKMLAVRIHRRSVTVGDITEKEASRVLQDSGLSAGTADEIFRM
                     TSLADYGDRFVIPPAHREEAVEAIENTFIRKENAGFGFREKPERGL"
     gene            complement(4457..>5131)
                     /locus_tag="PLZ75_10605"
     CDS             complement(4457..>5131)
                     /locus_tag="PLZ75_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOS72883.1"
                     /translation="SPIWSGLMTNGRAYSAFTLNIEQLIPWRTLTGRQHMYLDHEGYI
                     RFGENLPTYKPSPRPESYGDLRKTIPEGDALLLNVLTPHGKWHIHSTYGDTLRMLTLS
                     RGMEPCWLSEKDAERLGIRDNDWVEVFNDNGNYCTRACVSSRIPPGTCIIYHAPERTY
                     SVPKSKRSGNKRAGSHNSLIRVHLKPNLLVGGYGQFTFGMNYWGPVGVNRDTFALIRK
                     LDNVTF"
BASE COUNT         1316 a         1305 c         1153 g         1357 t
ORIGIN      
        1 ccgtggtacc ttccccgtta aatccggaaa gaggattccc ttcaagtttt accgggcgcc
       61 cgtcccttgt tttaaccagt atgtcggaat attcatgccc gtcataaaaa cttgttgcat
      121 aatacacgga ttcacccgga ctgaattccg gcggctgatt gacatagggc acagctttcc
      181 tgacaggtat tttgcatccc gcaatcagtg cagctgaagc aaagctgaat ccgaaagcct
      241 tgagaaaatc tctcctggaa ctgctcagcc ttaagaaatc ctctccgggc aattccggaa
      301 catgattatt atcttcctca ctgtttctgc ctccacctgc tgcaggatca ataaattctt
      361 ccataatatc gccggagttc ttcatattac ttgtctttat tgaaattcat ccatcagtaa
      421 tggcatttca tgcactcggt accacccact ctttccggag tgatgctgtc tgttacgcct
      481 ttccgcagtt tatctgatag ttcatggtat tgcgaataga aattattgcc gctgaaattg
      541 actttccgtg tacggtgaca tttaatacac cagcccatcg acagatcaga tacctgcctg
      601 atcctgacca tctttgcaac atccccgtgg cattccgcgc aatcaattcc tcctgcgcca
      661 acgtgctggg catgactgaa aaacacatga tccggaacat tgtgtaccct gatccattca
      721 atcggccttt tctcctcata gcttcctgta acctttgcta tttcaaacat gcccgacctg
      781 gttccgctgc gcaccagaag atgacagttc atacagatat tctgggccgg gattcccgct
      841 gacttgctgt aaggagcata attgtggcaa taaatgcagt ctgttccgtt ttgctccgca
      901 tgaatctgat gggaaaactt taccggctga tcaggtgaat agcccggaga acgcccgaga
      961 tcaatagcat tgacaacaag agaccaggta atatatactg ttgcaataga gagaacaaca
     1021 ggattaatcc agcgtttttt tatctttttg gtgattatca ggtcgactgc tgaaacaaaa
     1081 aacaggaaaa aggctgtgat gaacagaacc aggtttgtga caggaggctt cctttgcgtc
     1141 aggccggtac cggcaagctc atccatataa ccctttatca gcataatctc ctcaggggta
     1201 aaacggaaat cccggtgcac ctgtgccatc ttctttccgg tgggactgag caggatttcc
     1261 gtgagatcat caacactttt gtttttatat ttccccgata tctccaccgc atcaggattc
     1321 cagttcaggg tgtcggttac ggctgtattg tgacaggcag cacaattcat cgctttatta
     1381 tccggataaa ccagtccgta aaaaagtctc tgccccctga tcagttcttc tgaagcctgt
     1441 attactgacc cggatatgct tttcactgaa tcttctgact gcaattcctg tttgccggga
     1501 gcatcaggcg gcaggtcagc ggaagcagga tgcgaaaaac cagcggaaaa aataaaaaca
     1561 agtgatgaaa atgtcagggc ggataagccc ggggaatatt tccggctgca ggacttcctc
     1621 atggcatgga atttatgata atgtatcaat tgtcatttcg tgtttcagct ttttactgtt
     1681 caggcacttt cccttttgtt ccagatcact atctgatagc ggcgccagaa atacacaaag
     1741 gggtaaacca aaaaatgcat gaatcttgta taggggatca ttccgacaag aacaaaggcc
     1801 gaggcaatat gaatcttcac cataacggga agggcagtaa ctgctgcgac atcgggcctg
     1861 aaggcaaaga cagatttaag atagggagtc agcaccgtgg caaaccagga tgatccccat
     1921 ctgaaaaaca atgctgtata caagcctgtt acaatctgga ctgccagtat cagaaacacg
     1981 aaaacatcca ttctgcttgt aacgagccgg atccttttga ccctcaatcg tctgtaaacc
     2041 aatattaaaa atcctatcaa agtcatgaat ccaaaggaca gggcagccac ttcaagtatg
     2101 tacagccgga ccggtttttc gttccacgcc agtaccgacg ccgggatcag aaaagcgata
     2161 aggtggccga agaacaacag gatgattccc cagtgaaacg gcctgcttcc gaaaaacaac
     2221 agtttattct caaggagctg gctggagagg gaggataccc tgaatccatg taccctgtat
     2281 ttatatatgc ttgcaggaag cagggaaaaa agagccaggt aaggcagtaa aacaaaaaag
     2341 aaaaacccgt tcatttccat cctgtttgat ttttatttat tataatttca ttttcccgtc
     2401 cgccaaaggt gaatctttca aaagttgaaa agggaaaatc tgcttccatg attcctgcca
     2461 gaatctcaag caagccttta taaagattcg acatgtccct gaattccgca atcatcctgt
     2521 gtattgcagg aataagcatg gaacagatca gttcctcagc cagttcagga tcagccatcc
     2581 tgggcaggag ggtcagaata ttcggcagat gatcgggcaa ttcgctcccg cagtcatttt
     2641 ttgcctttat ctgttccttt ttaatattca ccaggaatat accccgcctg taatcctcac
     2701 cgtataaaac atagcctaca tccaggaaac acagggcctg cacatcaaag gtgccgacgt
     2761 agtattcctg ctgaaaggcc agcggctttc cccttacatg agcagtaaaa cggtccagcc
     2821 gtgacatcag ggatgcatca taacgggaaa caatgtcgtt ccacttctcc ggataattct
     2881 tcagatcatc cgtggggtac cggaacatct ccgccagcag accgtaatgc gttaaatctt
     2941 tcatgtcaaa gtcccctttc cggtttctcc ctgaatccga atcccgcgtt ctccttacgt
     3001 ataaatgtat tttcaatggc ttcaacagct tcttcgcggt gagcaggagg aataacaaac
     3061 ctgtcgccat agtctgcgag ggatgtcatt ctgaatattt catcagccgt tccggccgac
     3121 agaccggaat cctgcagaac gcgggaagcc tccttttcag ttatatcccc cactgttaca
     3181 gaacggcggt gaatcctgac agccagcatc tttttcagga tattcctgac cagttccgta
     3241 tcaccggcac tgaacaggtt tgccagatat ttcatgggca gccgtgagtc atccacattg
     3301 ttccataatg aactgcctgt agaactgtag aaattgtttt ccatggttgc catggccggc
     3361 aggagaggca gggcataaaa caggccgggc agggtccgga attccggatg aagaggcagg
     3421 gccagccccc attccttaac gtatttccat acgggtgatt tcctggcagc ctccattgtg
     3481 gagtcgggaa taccggactt ttttgcctga ctgatcacca cagggtcgtt cgggtcgaga
     3541 aaaagatcca gctgactccc cacaatatcc ctgtcctcag cagatgcagc ttccctgatc
     3601 cggtcagcat catagagcat tacccccagg tacctgatac gcccgacaca ggagtgcata
     3661 caggccggaa tctgcatggt ttcgatacgg ggatagcaaa ggatgcattt ctccgattta
     3721 cccgtattcc agttaaaata taccttttta tacggacatg cagtgacaca caatctccag
     3781 gccctgcatt tcttctggtt tatcaggact atgccgtctt ctccccgttt gtatatggca
     3841 cctgagggac aggcggcaac acaggccgga ttgaggcaat ggttgcagat tctgggaaga
     3901 tagaacataa ccatcctttc cagctgaaac agagcctcct tctctgtttc tgtcagatcc
     3961 gacagatttg tatcattcct ggcatagatg ggagatcccc cgaggtcgtc atcccagttg
     4021 ggccctgatt tcggagttat cctgtctcca gttataaggg atacaggctc tgccgtcggc
     4081 tgatcatccc cggccggtgc atccagaagc ttttcgtagc ggtaggtcca tggttcataa
     4141 tagttatcca gggcaggcag attcgggttt tgaaatataa cacccgttcc ctttattttt
     4201 ccggctcctt tcagtcttac cctgctgccg tccttttccc atccccccct gtataaatcc
     4261 tgttcttccc atctgcgggg ataaccggta cccggtttgg tctcaacatt gttccaccac
     4321 atatactctg cacccttcct gtcggtccat gcgttcttac atgcaatgga acaggtgtgg
     4381 catccgatgc acttgtcaag atgaaaaacg atggaaatct gagatttgat attcatggta
     4441 tgcctgattc ataaaattaa aaagtcacat tatcaagttt ccgtataagg gcaaatgtat
     4501 ccctgttcac ccccaccggt ccccagtaat tcattccgaa agtaaactgt ccgtatccgc
     4561 caaccagcag gtttggcttc agatgtaccc tgatcagact gttgtgactt cccgcccttt
     4621 tgttgccgct gcgtttcgat ttcggcaccg aataggttcg ttcgggagca tggtaaatga
     4681 tgcatgttcc cggaggtatc cgcgaactga cacatgcacg ggtacagtaa tttccgttgt
     4741 cattgaatac ttccacccag tcattgtccc tgatcccgag cctttcagca tctttttcgc
     4801 tcagccagca gggttccatg cccctcgaca gggtaagcat ccttaaagtg tcgccgtagg
     4861 tggaatggat atgccatttc ccgtgcgggg taaggacatt gagaagaaga gcatcccctt
     4921 ccgggattgt tttgcggagg tccccgtatg attcaggtct tggcgaaggc ttgtaggtgg
     4981 gcaggttttc accgaaacgg atatagcctt catggtcaag atacatgtgc tgacggcctg
     5041 taagggtccg ccagggaatc agctgctcaa tgttcagtgt gaaggctgaa taggccctgc
     5101 cgtttgtcat caggcccgac catataggag a
//
LOCUS       DAOSPX010000235         5124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14821_length_5124_cov_3.930953, whole genome shotgun sequence.
ACCESSION   DAOSPX010000235 DAOSPX010000000
VERSION     DAOSPX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14821_length_5124_cov_3.930953"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLZ75_10610"
     CDS             complement(<1..1171)
                     /locus_tag="PLZ75_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS72884.1"
                     /translation="MRESVLLALIHIFAIVSTVNPSGITARGKKILRSYLRRYLNREL
                     EEDYYALFENNLEFYSNELKGLDKAELADENSLISFQITNICRQIKKGLFLEERMIVF
                     LQLLEFAFEDGVISEQEKIIVDIIVRTFNIPRKEYRNATAFIVGRAFDDISTDSVLII
                     ESDRPELSGSKLYRNYDKWKHLKVKGFRGHMMVLYIESTETLLITYDGPVTLYFKGRD
                     IIAFRPYLLERGVNIKGLGIEPIYYSRIFKKFVSREFTEKVIFEGHDVQYHFRNSVNG
                     VQKMNFRVESGNFVGIMGGSGVGKTTMLNLLHGKIKPTGGHLYINGYDIYADSEQIKG
                     LIGYVPQDDMLIEELTVYQNLYFNARLCFGDYSEDQLNATVEKVLQDLDLYEIRDL"
     gene            complement(1237..2058)
                     /locus_tag="PLZ75_10615"
     CDS             complement(1237..2058)
                     /locus_tag="PLZ75_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72885.1"
                     /translation="MKNTLAALMLPLLLAGIVVTASNTSCKSRKKEKKIELEQAGVLV
                     DEIEKNVYPLPTSAEVIKMLTDLEVGYIFGISNPAENSKKYIKSTDRAINLGGYGADL
                     SYATLYNMQQEVLNYLGALRSLANELNMSQIYDESWYEKIRRNYDNRDELVKILTDAF
                     QNTYAYLVDNNQETLALLVVGGAWVEGLYLTTHVSEAAYQIAGISRNLLEQKKSFDTY
                     LEITRPYADDPDISEFLKILEPVRKVNEGLGTSLTQQNIIDITKAIEEIRARIIK"
     gene            2369..3544
                     /locus_tag="PLZ75_10620"
     CDS             2369..3544
                     /locus_tag="PLZ75_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOS72886.1"
                     /translation="MKWNFDEEINREGSDSVKYDLRYEKFGKRDIIPMWVADMDFRTP
                     GFITEKLRERLDHEIYGYFFRPPQYYDSLISWLNTRYCSPVERDHICFSPGTVPAINL
                     CTLTFTRPGEAVIVQPPVYYPFFSAVESHGRVLVNNQLREKDGRWTMDLEALRQMVTA
                     ETKMIILCNPHNPVGRAWSREELMELARICLKNNIIVLSDEIHCDLTLPGNKHTPFAT
                     LSDEIADITVTCIAPSKTFNLAGLSTSSVIISNPVLRKYFRHKVDSLHIGNGNIFGSV
                     ASEAAYSGGHEWLDALTAYIEGNVNHVTDYCREFIPEIIPVRPEATYMIWLDCRKLGM
                     TGKELRKFFVEEAGVGLNEGSEFGPGGEGFMRMNLAMPRSRVEKAMQQIGKAISRIP"
     gene            complement(3525..4865)
                     /gene="argH"
                     /locus_tag="PLZ75_10625"
     CDS             complement(3525..4865)
                     /gene="argH"
                     /locus_tag="PLZ75_10625"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOS72887.1"
                     /translation="MKLWEQGINPEPAIIEFTAGRDRKIDIHLTKWDIVGSMAHVIML
                     HDVGLIDNEEKKNILKALHSLFVWDEEGSLAIESGIEDIHSQIEKEMASILGTTGRKV
                     HTGRSRNDQVLVDIRLYTRERIDSMALLAGRLFRKFQSLSEQYREVLLPGYTHMQPAM
                     LSSFGLWFGSYAESLSDDVALLATARALNNRNPLGTAAGFGTTLPLNRKLTSELLEFD
                     DLIYNSAYASLNRGKVETAVASALAALAQTLSRFSMDVCLYMSAEYRFVDFPDEYVTG
                     SSIMPQKRNPDVIELIRGRANLIQTLPGQVAMLVSNLPSGYNRDLQLLKELVFGAFED
                     LMECMEITLLMLNNIRIRKNILENPIYNTIFSTEEVNRLVKQGIPFRDAYKAVSGMVR
                     NSSFSRTALSDYVHEGSPGNLCNEAIATRFSESMKCFRNKSAAEMAGKLMEYAK"
     gene            complement(4877..>5124)
                     /locus_tag="PLZ75_10630"
     CDS             complement(4877..>5124)
                     /locus_tag="PLZ75_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOS72888.1"
                     /translation="DFLMAAIEKSQELIDGSVHLCLYKGNVLIEGRESPSSLYDEKLS
                     SMDIEGGFDQTDSLGFIKINAIRLMAHRAIVEQRQKK"
BASE COUNT         1309 a         1224 c         1154 g         1437 t
ORIGIN      
        1 ggagatccct gatttcataa aggtccaggt cctgaagcac tttctccaca gtagcattaa
       61 gctggtcttc cgaataatcc ccgaaacaaa gccgtgcatt gaaatagagg ttctggtaaa
      121 ccgtcagttc ttcaatcagc atatcatcct gcggaacata gcctatcagt ccctttatct
      181 gctcagaatc ggcatagatg tcataaccgt ttatatagag gtggcccccg gttggcttta
      241 ttttcccgtg aagcaggttt agcatggtgg tttttcccac tccgcttccc cccattatac
      301 ctacaaaatt acccgattcg accctgaaat tcattttctg taccccgttc acggaattcc
      361 tgaaatgata ctgaacatca tgaccttcaa aaatcacctt ttcggtaaat tcccttgaaa
      421 caaacttttt gaagatccgt gagtagtaaa tgggttcaat gccgagtccc ttgatattga
      481 cgcctctttc aaggagatag ggtctgaagg cgattatgtc gcggcccttg aaatagagag
      541 ttaccgggcc gtcgtatgta ataagcaggg tttcggtgct ttcgatgtaa aggaccatca
      601 tatgacccct gaaacccttt accttcagat gcttccattt gtcatagttc ctgtacagtt
      661 tagatccgga aagttccggt ctgtcacttt cgataatcag aacggaatca gtggagatat
      721 catcaaatgc cctgccgaca atgaatgccg tggcattcct gtattccttc cggggaatgt
      781 tgaaggtcct gacaattatg tcaacgataa tcttttcctg ctctgaaatc acgccatcct
      841 caaatgcaaa ttccagcagc tggagaaaaa caatcatcct ttcctcgaga aacaatccct
      901 tcttgatctg acggcagata tttgttatct gaaaggagat cagggagttt tcatcagcaa
      961 gctcggcctt gtcgagacct tttaattcat tggaatagaa ttccagattg ttctcaaaca
     1021 gtgcatagta atcttcttca agctctctgt tcagatatcg ccggaggtag ctccgcagaa
     1081 tttttttgcc tcttgccgtt atccctgacg ggttgaccgt tgaaacaatg gcaaagatat
     1141 gaatcagggc caacagtact gattccctca tatggatgcc ggttcaaaaa aagaccgcaa
     1201 agtattattc cctgcggtca aatgtctgta aaaagattat tttatgattc tggcccgtat
     1261 ctcctcaatg gcctttgtaa tatctatgat attctgctgg gtaagactgg ttccgaggcc
     1321 ttcattcact tttctgacag gttccaggat tttcagaaat tcacttatat ccgggtcgtc
     1381 agcatagggt ctggtaattt ccagataggt atcgaaggat ttcttctgtt cgagaaggtt
     1441 cctggatatt cctgcgatct ggtatgctgc ttcggaaacg tgagtggtaa gatacaggcc
     1501 ttccacccat gctcctccga ctacaagcag ggccagggtc tcctggttgt tgtcaaccag
     1561 gtaggcatat gtgttctgga atgcatcggt cagtatctta acgagctcat cacggttgtc
     1621 ataattgcgc cttatctttt cataccacga ttcatcatag atctgcgaca tgttcagttc
     1681 attggccaga gaacggagag cgcccaggta attcaatact tcctgctgca tgttgtacag
     1741 ggtggcataa ctgagatcag ccccgtaacc acccagattg attgcacgat ccgtgctctt
     1801 tatatatttc ttggagttct cggcaggatt tgaaatgcca aatatataac caacttccag
     1861 atcggtcagc attttaataa cctcggcaga tgtgggaagc ggataaacat tcttttctat
     1921 ttcatccacc agtacgccgg cctgttcaag ttcaattttc ttctccttct tcctgctttt
     1981 gcatgaagtg tttgaagccg tgacaacaat tcctgccagt aataatggaa gcatcaatgc
     2041 tgcaagtgta tttttcatcc ctgatcaatt tattgttaac agtacattta aaactgccga
     2101 taaaaatagt aaattaatta ttaaatctgc acgactgtat cagttattta ttcattatat
     2161 cccttgtctt atcaactgta tctgaaataa ttctataaac cggaaatgag ccggctcctg
     2221 aaagtctgaa tatcttgcat gtcaggaagg cctcagccgg cagatcctcc cctgttttac
     2281 tgaaacagca ggcttttaag gggaataggg taaaggaaat ctgattttga tacctatctt
     2341 tgaagccccg taaaattatt atattcagat gaagtggaat tttgacgaag agatcaacag
     2401 ggaaggcagc gattctgtca aatatgatct gagatacgaa aaattcggaa aaagggatat
     2461 tattcccatg tgggttgccg acatggattt caggacgccc gggttcataa ccgaaaaact
     2521 gcgggagcgc cttgatcatg agatatacgg atactttttc cgccccccgc agtattacga
     2581 ttcactgata tcctggttaa acacccgtta ctgctcgccg gttgaaagag atcatatctg
     2641 cttcagtccg ggtaccgtac cggcaataaa tctctgcacc cttacattca cgcgtccggg
     2701 tgaggctgta attgttcagc ctcccgtcta ttacccgttt ttttccgccg tggaaagcca
     2761 tggaagggtg cttgtcaata accagctgag ggagaaggac ggaaggtgga ccatggatct
     2821 tgaagccctc aggcagatgg tcacagccga aacgaaaatg attatccttt gcaaccctca
     2881 taatcccgtt ggaagggcat ggagccgtga agagctcatg gaacttgccc ggatctgcct
     2941 gaaaaacaac ataatagtcc tttccgacga aatccactgc gatcttaccc ttccgggaaa
     3001 caaacatact ccttttgcaa cactttcgga tgagattgct gatataaccg tgacatgcat
     3061 tgctcccagc aagacattca atctggcggg gctttccact tcctcagtga ttatcagcaa
     3121 tcccgtactg aggaaatatt tcaggcataa agttgacagc ctgcatatag gcaacggcaa
     3181 cattttcggc agtgttgcct ccgaggcagc ctacagcggg gggcatgaat ggcttgatgc
     3241 ccttaccgcc tacatagaag ggaatgtaaa ccatgttact gactactgca gggagttcat
     3301 tccggaaatc attcctgtca ggcccgaggc aacctatatg atatggctcg actgcagaaa
     3361 gctcggaatg accggaaagg aactccggaa attctttgtg gaagaggccg gagtcggtct
     3421 gaacgaaggt tcggaattcg gtccgggcgg agagggtttt atgagaatga atctggcaat
     3481 gccgcgcagc agggtggaaa aggcaatgca gcagatcgga aaggctattt cgcgtattcc
     3541 ataagctttc ctgccatctc tgccgcggat ttgtttctga agcatttcat gctctcactg
     3601 aatcgtgttg caatggcttc gttgcagagg ttccccggac tgccctcatg aacatagtcg
     3661 gacagtgccg tcctggagaa ggaagagttt ctgaccattc ccgatactgc cttgtaggcg
     3721 tccctgaagg gtattccctg tttgaccagg cggttaacct cctcagtgct gaatattgta
     3781 ttgtaaatgg gattctcaag gatatttttc ctgatcctga tattgttgag catcaggagc
     3841 gtgatttcca tgcattccat caggtcctcg aaggcaccaa aaacaagctc tttaagcaac
     3901 tgcaggtcac ggttgtatcc tgaaggaaga tttgaaacca gcattgcgac ctgtccgggc
     3961 agggtctgga tcaggttggc tcttccgcgt atcagttcaa taacatctgg attgcgtttc
     4021 tgaggcatga tacttgatcc cgtgacatat tcgtcaggaa aatcaacgaa gcggtattct
     4081 gcactcatgt agaggcacac atccattgaa aagcgtgaaa gggtctgggc cagtgcagcc
     4141 agggccgatg caacggctgt ttcaaccttg cccctgttca ggctggcata ggcagaatta
     4201 tatatcagat catcaaattc cagcagttcc gatgtgagtt tcctgttaag cggcagggtg
     4261 gtcccgaatc cggcagctgt tcccagagga ttacggttgt tgagggcacg ggctgttgca
     4321 aggagggcaa catcatccga cagcgattcg gcataggatc cgaaccacag tccgaaagat
     4381 gacagcattg ccggctgcat gtgagtgtag cccggcagca gaacctcccg gtactgttca
     4441 ctcagcgact ggaacttccg gaagagcctg cctgcaagca gcgccatgct gtcaatcctc
     4501 tccctggtat acagtcttat atccacaagc acctggtcat ttctcgatct tccggtatga
     4561 acctttctgc cggtggtccc gagaatagaa gccatttcct tttcaatctg cgaatgtatg
     4621 tcttcaatac cgctctcaat ggccaggctg ccttcctcat cccatacaaa cagtgaatga
     4681 agcgccttaa gtatgttttt cttttcctca ttatcaatca ggcctacatc atgaagcatg
     4741 atcacatgag ccatggatcc gacaatatcc catttggtga gatgaatatc gatcttcctg
     4801 tcccgacctg ccgtgaattc gataatggca ggttcggggt tgataccttg ttcccagagt
     4861 ttcattttcc tttagtttac tttttctgtc tctgctcaac gattgccctg tgagccatga
     4921 gccttatggc attgatctta ataaatccca gactgtcggt ctggtcaaat cctccttcaa
     4981 tatccatgct tgacagcttt tcgtcataga gggacgaggg tgactccctt ccttcaatga
     5041 gaacattccc cttataaagg cagaggtgta cacttccgtc aatcagttcc tggctttttt
     5101 ctattgccgc catcaggaaa tcca
//
LOCUS       DAOSPX010000236         5091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14931_length_5091_cov_4.875099, whole genome shotgun sequence.
ACCESSION   DAOSPX010000236 DAOSPX010000000
VERSION     DAOSPX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14931_length_5091_cov_4.875099"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PLZ75_10635"
     CDS             <1..1204
                     /locus_tag="PLZ75_10635"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOS72889.1"
                     /translation="FWSKILHVTRKNRRIRENAFSWVEHCDWIPALLTGNTDPLKLKR
                     SRCAAGHKAMWHKNFGGLPSEDFLTGLDSGLAGLRDRLYTETFACDVSAGTMSAEWAK
                     KLGLPPGIKVGVGAFDAHLGAVGGGISPGQLIKVMGTSTCDMLVSPFTGEEERLVRGI
                     CGQVDGSIIPGMLGLEAGQSAFGDIYAWFGTLISWPVSEIIGKMSWLDSRTRKRIKSE
                     TSEAMISELSRAARQVLTEESAVLALDWMNGRRTPDANQALKGAIAGLSLGSDAPRIF
                     RALVEATAFGSRMINERFVSEGMKIDGVIAVGGVAKKNPFVMQVVSDVLNMPIKAAKA
                     EQPSALGSAMAASVIAGIHADIPAAQKAMGSGFEKTYRPDPARAAKYDVIFKEYMKFG
                     AFIENEMT"
     gene            complement(1453..3999)
                     /locus_tag="PLZ75_10640"
     CDS             complement(1453..3999)
                     /locus_tag="PLZ75_10640"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HOS72890.1"
                     /translation="MKIISLTLIHLFTCFVIFAQEWQPQPWPVLKTYDQNHLFQVALP
                     LGGIGTGTVSLGGRGELRDWEIMNIPGKKYSTVTTGLNAPFFAVYVESSDGTSSATLL
                     TGSLESHEYLHYEGRPVNHHGFPRFSNAKFSAAYPFGQVSLSDEKLPVTVEIKGFNPL
                     IPGDADGSGLPVAVLTYEVTNKSNRPLEVAVCGSIRNFIGKDGSKFRTDWKGDYIPTG
                     AKQNKNEYREGKNNLRGIYFYSEGVEKDDPAWGTMALVTEASAGISYRTASVLDHWNN
                     AMLNFWDDFSDDGVLEDVHNQIEDDPMASLSVKKRILPDQTEAFTFFLVWNFPNRKAW
                     SPSMVGNYYSSKYEDAWDAAERIVPGIPNLEDQTLRFVNAFLSSTYPDVVKEAALFNL
                     ATLRSQTVFRLPGGHMMGWEGVMDRFGSCAGSCTHVWNYEVATPFLFGDLAMTMRDVE
                     FNYATKENGLMNFRASLPLSEASKGNSAAADGQMGCIMKMYREWQLSGDNNFLRDNWG
                     QIKKVMSYAWVEKGWDGNQDGVMEGRQHNTMDVDYFGPNPQMGFWYLGALKAAEKMAL
                     AMKDKEFGRKCKDLFNKGSEWMDKNLFNGEYYEHKITDPETFGFLDMESGNTQIPAFQ
                     LGKGCLVDQLVGQYMAHICGLGYLADDNNIRTTLKSIMKYNYVEDFSAHFNNMRSYVM
                     GNESGLLMASWPKGRLKVPFPYFAEVMTGFEYSAAVGMIYEGMEHEALKCIESIRERH
                     DGAKRNPFSEPECGHHYARSMASWASIIALSDFQYSGVDHCMQFTAKPGNYFWSNGYS
                     WGTCAVSDDTVTLEVLKGSLNSIKLTLTGRKKAIGNKISLKEGEKVTLKM"
     gene            4434..4697
                     /locus_tag="PLZ75_10645"
                     /pseudo
     CDS             4434..4697
                     /locus_tag="PLZ75_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4759..5091
                     /locus_tag="PLZ75_10650"
                     /pseudo
     CDS             4759..5091
                     /locus_tag="PLZ75_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799975.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
BASE COUNT         1447 a         1190 c         1061 g         1393 t
ORIGIN      
        1 gttctggtca aagatactgc atgtaacccg gaaaaaccgc aggatcaggg aaaatgcatt
       61 ttcatgggtt gaacattgcg actggatacc tgcactgctg accgggaata ctgatccgct
      121 gaaactgaaa cgcagccgtt gtgctgccgg tcataaggca atgtggcaca agaatttcgg
      181 aggtctgcct tccgaagatt tccttacagg gctcgattcc ggcctggcag gtctgaggga
      241 caggctctat actgaaactt ttgcctgtga cgtctctgca ggtacaatga gtgcagaatg
      301 ggcaaaaaag cttggcctgc cgcccggcat aaaagtaggg gtgggagcat tcgatgcaca
      361 tctcggagcc gtaggcggag gaataagtcc gggacagctg atcaaagtca tgggaacctc
      421 aacatgcgac atgctggttt ctccttttac aggagaagag gaaaggcttg tcaggggaat
      481 atgcggacag gttgacggat caatcattcc cggcatgctt ggcctggaag ccggtcagtc
      541 tgctttcgga gatatctacg catggttcgg aacacttatt tcatggcctg tttcggagat
      601 tatagggaaa atgagctggc ttgacagcag aaccaggaaa agaataaagt cggaaacgtc
      661 ggaagcaatg atatctgaac tcagccgggc agcccggcag gttctgacag aggagagtgc
      721 cgttctagca cttgactgga tgaacggccg gcgtacccct gatgccaatc aggcactgaa
      781 gggcgcaata gccggattgt cactggggtc ggatgcaccc cggatattca gggcactggt
      841 ggaggccacc gctttcggat caagaatgat caatgagcgc ttcgtttcgg aaggaatgaa
      901 aatagacggt gtaatagccg tcggcggagt tgcaaaaaag aatccctttg tcatgcaggt
      961 tgtttcggat gtactcaaca tgccaatcaa ggctgcgaag gcagaacagc cttccgcact
     1021 cggatctgcc atggcggcgt cggtgatagc cggaatccat gcggatatcc ctgcagccca
     1081 gaaagcaatg ggaagcggat ttgaaaaaac atacaggccg gatccggcaa gggcagcgaa
     1141 atatgatgtg atttttaaag aatacatgaa attcggtgct ttcattgaaa atgaaatgac
     1201 ctgacctgaa ctgacctgac ctgacctgat atgacctgat atgacctgac ctgatctgac
     1261 ctgagattct gtcagactca gcatcgcgcg gtcttcccgg gagatattta ttaaaagatg
     1321 atctgactta tctggcaaag gagctggata ttcgagttga actgatctat cattgaagac
     1381 gggaagcttc ggcaaatttg ggtgccagct tccctggcca ggggaacagg tcccattgca
     1441 tattctcagc tattacatct ttaatgttac tttttcccct tccttaagag aaatcttatt
     1501 tccaatagct ttcttcctgc ctgtgagtgt cagtttaatt gaattcaatg atcctttcag
     1561 cacttcaaga gtaacggtat cgtccgaaac cgcgcaggtg ccccatgaat atccattgct
     1621 ccagaaataa ttccccggct tagcggtaaa ttgcatacag tgatcaaccc ccgagtattg
     1681 aaaatcactc aaagcaataa tacttgccca gctagccatg gagcgagcat aatgatgtcc
     1741 acattccggc tcactgaaag gatttctttt agcaccatca tgacgttcac gaatagattc
     1801 aatacatttt agagcttcat gttccatacc ttcataaatc ataccgacag ctgcactata
     1861 ttcaaaaccg gtcattactt ctgcgaaata agggaaaggt acttttaaac gtccttttgg
     1921 ccaggaggcc attaacaagc ccgattcgtt ccccatcaca taagatcgca tattattaaa
     1981 atgggcacta aagtcttcca cataattgta tttcattatg ctcttcaatg tcgtccggat
     2041 attattgtcg tctgccaggt atcccaaccc gcagatatga gccatatatt gccccaccaa
     2101 ctggtccacc agacaacctt ttcccaattg aaaggcaggg atttgcgtat ttccggattc
     2161 catatccaaa aacccgaatg tttccggatc cgtaatctta tgttcgtaat actctccatt
     2221 gaaaaggttt ttatccatcc actcactacc tttattgaac agatccttac actttctccc
     2281 gaactccttg tctttcattg ccagggccat cttctcggca gctttcaaag cacccaaata
     2341 ccaaaatccc atttgaggat taggaccaaa gtaatcaaca tccattgtat tatgctgacg
     2401 tccctccata acaccatcct gattcccatc ccatcctttc tcaacccatg catatgacat
     2461 aacttttttg atttgccccc aattatctct taagaaatta ttatcacccg aaagttgcca
     2521 ttctctgtac attttcataa tacaacccat ttgcccgtca gctgccgcgg aatttccttt
     2581 cgaagcttca gacaaaggta gtgatgcacg aaaattcatt aaaccatttt ctttggttgc
     2641 ataattaaac tcaacatctc tcatcgtcat tgccagatca ccaaaaagaa aaggggtagc
     2701 gacctcataa ttccacacat gagtacatga gccggcacaa gaaccaaatc ggtccatcac
     2761 accctcccat cccatcatat gaccaccggg caaccggaat actgtttgag agcgcagggt
     2821 ggcaaggtta aacaaggctg cctctttgac tacatcaggg taagtactgc tcaggaatgc
     2881 attcacaaac ctcagtgtct ggtcttccag gttgggtatc ccgggaacga ttctttcagc
     2941 agcatcccat gcatcctcat actttgaact ataataatta ccaaccatag atggagacca
     3001 cgcttttctg ttgggaaagt tccacacaag gaaaaaagtg aatgcctcag tttgatcagg
     3061 cagtattctt tttttcaccg acaatgaagc catgggatca tcttcaatct gattatgaac
     3121 atcttccaga acaccatcgt cactaaaatc atcccaaaag ttcaacatgg cattgttcca
     3181 gtggtctaga acagaagcag tgcggtatga tatcccagct gatgcttccg tcaccaatgc
     3241 catggttccc catgcgggat catccttttc cacaccctcc gaatagaagt atattcctct
     3301 gagattattt tttccctctc tatattcatt tttattttgc tttgcgcctg tgggaatata
     3361 atctcctttc cagtcggtac gaaacttact gccatcttta cctatgaaat tacgaatcga
     3421 tccacaaaca gccacttcaa gtggcctgtt acttttgttc gtcacctcat atgtaagaac
     3481 agcaacaggc aaaccacttc catcagcatc gcccggtatc agtggattaa atccctttat
     3541 ttccacggtg acaggcaatt tttcatcaga taaagatact tgtccgaatg gataagcagc
     3601 actgaactta gcatttgaga aacggggaaa accatgatga tttaccgggc gcccctcata
     3661 atgaagatat tcgtgggact caagagaacc tgtaagcagt gtggcagaag atgtgccatc
     3721 cgaagattca acataaacgg caaaaaaagg agcattcaga ccagttgtaa ctgtactgta
     3781 tttttttccg ggaatattca ttatttccca atcacgcagt tcacctcttc ctcccaacga
     3841 gacagtacct gtaccaattc cacccaaagg cagagctacc tggaataaat gattctggtc
     3901 ataggtttta agaaccggcc agggttgggg ctgccattcc tgtgcgaaaa tcacgaagca
     3961 tgtaaacaga tggattaaag taagactgat tattttcatt gttacttgtt ttttatagtc
     4021 aagggagtca ctataataca actccattaa cttttcattg aatttatttt tttcccaatt
     4081 tggattttga gtcaaatttg gattattatc cggagcactt tccgaaagtg gccataaaaa
     4141 atgatttgaa ggatcaaatg tgcgatttta ttcaaataat ttgtagcggg ataatgaatc
     4201 ccctgacaat tatatcccga accaccttct ccccatgcgt ttgtcatcaa agaattacaa
     4261 acacatcagc tcactgacgg gacacgcgtg cggatcaaac cgtgtagtct taatgagagc
     4321 gaggtaatga tcatagtaat ttacttccat ttaatgcgtt acagggactt aaaacattac
     4381 tgtctttttc acgtgtgcga gcatatgcgg gatgattttc cttcgtttac ggtatccgcc
     4441 tgattaccaa actgagaaag aacatgaaaa actccctgat gcttatatca gacaagatcc
     4501 tgcttcgtaa acgtgcgctg atcgaatctg ttatcgatga acttaaaaat atctgccaga
     4561 tagaacacac tcgttataga agtcaggaag ggtttattat caatttgtta tccggattaa
     4621 ttgcatatta ttacttaccc gagaagccat cacttaatcc ggagattatt gatgaatcaa
     4681 tggttttata tttttaactt tatatcgaac tcattttaat ataatatcat cagctataaa
     4741 ccctataaaa gaaaaactat gcgaaaactg cttttattat tcttagccgc ttccttcgga
     4801 atggtcaata tcagttccca aaaggagaat tctgatccgg tcaatcagct tgttattatt
     4861 gccaacaacc ccgggccggt tatcagcaag gatatttacg gtcatttttc ggaacatctc
     4921 ggaacgtgca tttatggcgg tatctgggtt ggcccggatt cgaggatacc aaatactcat
     4981 ggtataagga atgatgtcct ttatgcgctc agggagatca aagttcccaa tctccgctgg
     5041 ccgggcggat gttttgccga tacctaccac tgaaagacgg gataggacca a
//
LOCUS       DAOSPX010000237         5089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14943_length_5089_cov_3.968017, whole genome shotgun sequence.
ACCESSION   DAOSPX010000237 DAOSPX010000000
VERSION     DAOSPX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14943_length_5089_cov_3.968017"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLZ75_10655"
     CDS             <1..334
                     /locus_tag="PLZ75_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS72891.1"
                     /translation="DPSHLLWQQMDYIKPLYEFRDKLFHIHLKDARIYPEKLNEVGIM
                     AYPLEFHTPKLPGLGDIDWRKFFSALNDIRYDGPVCVEVEDKSYEGTLEARKYSLDLC
                     KRYLDTIV"
     gene            404..2518
                     /locus_tag="PLZ75_10660"
     CDS             404..2518
                     /locus_tag="PLZ75_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS72892.1"
                     /translation="MKNKAALFLAVIILLNCLQHTSGQIIYISPDGNDSGSGTLENPL
                     SSLNAALERMKVLKKDGLNEDNPRIIVLDGNYFLTEPIVLTEEHGGTADKPLVIKADA
                     GARPVFMAGMKTGRFEKINDRLWRVFIPRVAWQDLYFEQLYVNGRRAVRARTPNEGFY
                     TVRDVEETVFEKGEGRYPEYAVQKIQLGSEGAECFESFTDQDFRDAIVTFYHKWDNTR
                     KRAEHYSSSESAIYISGEGMKPWNSLDSKTRYRVENYKAALDSPGEWYLEKSGYLYYV
                     PLPDESIETTEAYAPLTEEFLIVRGASSERPVSNVRFENISFRVSGYRTPRTGNEPSQ
                     AAAAIDAAIRLDYARNISFIDCEVAHTGTYAFWFRRGCAGCTVNHCYIHDLGAGGIKI
                     GETILREDPSEITNNITIDNNIITDAGHVFPCAVGIIIFHASDNNLTHNEISNLRYSG
                     ISAGWIWGYAHSPSKRNIIRYNHIHHLGWGELCDMGGVYTLGASEGTCVSDNLIHHVY
                     SFDYGGWGLYTDEGSYGITMENNLVYACKSSGFHQHYGRENVIRNNIFALNIRSQLQA
                     TRIEDHRSLSFTNNIVYFDRGSLLSSNWHRFSLLSDYNCYWDIRTKDIYFAGMTFREW
                     KSEGKDVHSLIADPLFMDPAKFDFRFRKLTVARRIKFTPFDYTLAGVYGTDEWRKLAE
                     TDTDLTEKFDIIVNRMEGRSGY"
     gene            2613..>5089
                     /locus_tag="PLZ75_10665"
     CDS             2613..>5089
                     /locus_tag="PLZ75_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOS72893.1"
                     /translation="MKILAILFFLFTASFQCLHAKIFPTRLTCEYLTDPPVIDAANPR
                     LSWVNIAEEGERGQIQTAWEIRVAGTREQLISGSAGLWSSGKVLSDQSFNVRYGGKPL
                     VSGQECWWQVRVWDKNDKISGWSEPAFWRMGLLREADWKAKWIGPPWQGEEALPKPSN
                     PGAGLPGQLPPPAPLLRKEFSVSGKVARAVAYVTGLGYFEFWLNGEKVGEDVLVPNQT
                     NYGKRPSLPLENIPLDDNFREYRVMYLAYDVSDKLTEGPNVLGAILGNGFYNPAKYWA
                     GSYGTPRFIMQLHISYDDGSEETVISDETWKCSESPILMDMVFYGEHYDARLEQAGWC
                     SSGFKDYGWENAVLKKAPEGKMIAHTACPDRVMEVLKPVKTEKTGEGNYRIDFGQEIS
                     GWVHLKNMNGEAGRKIEIRYIHRVPSGENSYIMKGSGDESYHARFNWFVFREVEILNW
                     PGELKDGQVTAEAVYTRIETSGKFKCSAGLLEDINRIWWRSQTDNMHGGIASDCPNRE
                     RSPYTGDGQAACVTVIHNFDARAFYNKWIHDIIGASNPQTGYVPNGAPWQPGCGGGVA
                     WGAAICIMPWEFWVHYGDRDILRDSYEAMKGYVKYMLSWTDEKGIMFSQAVGRDGKVL
                     RWMNLGDWMAPYKLPPDNMVHTFYLWYCADIISRVAGILGNHQDEAIYGNLAEKTRTA
                     FMSEFYDQVNGTFGRYGGNIIALKMGVPEEIKKKVIKVLADDIKADKGNLDTGIFGTR
                     FFFEVLSANGLHDLAFEAMNKRTMPSYGYWLEQGATTMWEQWDQPLSGNHPMFGGGIV
                     WLYRNLAGMNADPADPGYRRIVFCPGPV"
BASE COUNT         1437 a         1033 c         1369 g         1250 t
ORIGIN      
        1 cgacccttca catttactgt ggcagcagat ggattatata aaaccgcttt atgagttcag
       61 agacaagttg ttccatattc atcttaagga tgcaagaatt tatcccgaaa aactgaatga
      121 ggtaggcata atggcttatc ccctggaatt tcataccccc aagctgccag gactgggaga
      181 tattgactgg agaaaattct tttcggcact gaatgatatc agatatgacg gaccggtgtg
      241 tgtggaggtt gaagataaat cctatgaagg caccctggaa gcaagaaaat attccctcga
      301 cctgtgcaag agatatcttg acacaattgt ataaatctgt ttttcagata gttaaaatag
      361 ttcagatagt tcagatagtt tgtgggatta aaatacatat gaaatgaaaa ataaggcggc
      421 tttatttctg gcagtcataa tattgttaaa ttgtcttcag catacttcgg gacagataat
      481 ttatatttcc cctgacggaa atgattccgg ttccgggact cttgaaaatc ccctgtcaag
      541 cctgaatgct gcactggaaa ggatgaaagt cctcaagaag gatggtttga atgaagacaa
      601 tccccggata attgttcttg acggcaatta tttcctgacg gaacccatcg tgctgacaga
      661 agagcatggc gggacagctg acaaaccact ggtgataaag gcagatgccg gagcaaggcc
      721 ggtattcatg gctgggatga agacaggcag atttgagaaa atcaatgaca ggttgtggag
      781 ggtctttatt ccccgggtgg catggcagga tttatacttt gaacagttat atgttaacgg
      841 aagaagggct gtcagggccc gtactccgaa tgaaggattc tacacagtca gggatgttga
      901 agaaacggtt tttgaaaaag gggagggccg ttatcctgaa tatgccgtac agaaaattca
      961 actcggcagc gagggggcag agtgctttga atcctttacg gatcaggact tccgggatgc
     1021 cattgtgact ttttaccata aatgggacaa tacaagaaag agagcagagc actacagcag
     1081 cagtgaatca gcgatatata tttcagggga aggcatgaag ccctggaatt cccttgacag
     1141 caaaaccaga tacagggtgg agaactacaa agccgccctg gattctcccg gtgaatggta
     1201 ccttgaaaaa tccggttacc tctactatgt tcccctgccg gatgaaagca ttgagacaac
     1261 tgaagcatat gcaccgctca cggaggaatt cctgattgtc aggggagctt cgtcagaacg
     1321 gcctgtcagc aatgtcaggt ttgagaacat ctcattccgg gtgagcggct accggacacc
     1381 ccgtaccgga aatgaaccct ctcaggcagc tgcagcgatt gatgctgcaa taaggctcga
     1441 ttatgcaagg aatatttcct ttatagactg tgaggttgcc cataccggca cctatgcttt
     1501 ctggtttcgc aggggatgtg ccggttgcac tgttaatcat tgttatattc acgatcttgg
     1561 cgcaggaggc attaaaatcg gtgaaacaat tctgagggag gatccctctg aaattacaaa
     1621 caatatcacc attgataaca atataattac tgatgcggga catgtgttcc cgtgtgcagt
     1681 cgggataata atattccatg caagcgataa caatctcaca cataatgaaa tatcaaacct
     1741 gcgatattca gggatctcgg caggatggat atggggatat gcccacagcc cctcaaagag
     1801 gaatattatc agatacaatc atatacatca tctcggatgg ggagagcttt gcgatatggg
     1861 cggggtatat accctgggtg cttccgaggg aacatgtgtg tcggataacc tgattcacca
     1921 cgtttattca tttgattacg gcggctgggg attgtacact gatgaaggtt catacggcat
     1981 aacaatggag aacaaccttg tatatgcctg caaaagctcg ggcttccatc agcattacgg
     2041 cagggagaat gttatacgga acaacatctt tgccctgaat ataagatccc agctgcaggc
     2101 taccaggatc gaggaccaca ggtcactgtc ctttacaaac aatatcgttt attttgacag
     2161 gggaagcctg ctgagcagca actggcacag gttcagtctt ctgtctgact ataactgtta
     2221 ctgggacatc aggacaaagg acatttattt tgccggcatg acgtttaggg aatggaaatc
     2281 ggaaggaaag gatgttcatt ccctgattgc agatcccctg tttatggatc cggcaaaatt
     2341 tgatttcagg ttcagaaagc ttactgtcgc ccggagaata aaattcacgc cctttgatta
     2401 taccctggcc ggagtatatg gtactgacga atggaggaag ctggcagaga cggacactga
     2461 tctgacggaa aagttcgata ttattgtgaa tcgcatggag ggccggtcag ggtactgatc
     2521 tctcctggaa ccatcatgga aatattggtt attcgggaga ctgatgaata aatcaatgga
     2581 tatccaagtt ccatttttaa tattaacttc tcatgaaaat acttgccatc cttttctttt
     2641 tgtttacagc atcatttcaa tgcctgcatg ccaagatttt tccaaccagg ctgacatgtg
     2701 aatacctcac tgatcccccg gtgattgatg ctgcaaatcc gaggctttca tgggtaaata
     2761 ttgcagagga gggtgagagg gggcagattc agacagcatg ggagataagg gttgcaggga
     2821 cgagagaaca gctcatttca ggtagtgccg gattgtggag cagcggcaag gttttgtcgg
     2881 atcagtcgtt taatgtgagg tatggcggca aaccgcttgt ttccggacag gaatgctggt
     2941 ggcaggtgag ggtatgggat aaaaatgaca aaatatccgg atggagcgaa cccgcgttct
     3001 ggagaatggg ccttctcagg gaggctgact ggaaggcaaa atggattggt ccgccgtggc
     3061 agggagagga agctctgccg aagccatcaa atcccggtgc cggtctgccc ggacaattac
     3121 cgccgcctgc accacttctc agaaaagaat tcagtgtttc ggggaaggtt gcccgtgcgg
     3181 ttgcttatgt taccggactg ggatattttg agttctggct gaacggggaa aaagttggtg
     3241 aggatgtcct ggtaccgaat cagacaaact acggaaagcg gccctccctg cctctggaaa
     3301 atattcccct ggatgataat ttcagggaat acagggtgat gtacctggcc tatgatgtaa
     3361 gtgataaact tacagaggga cctaatgtgc tgggagcaat tctggggaat ggtttttaca
     3421 atccggcaaa atactgggca ggctcatacg ggacaccccg ttttatcatg caactgcata
     3481 tcagctatga tgacggaagt gaggagactg ttatcagtga tgaaacatgg aaatgttcgg
     3541 aaagtcccat acttatggat atggtctttt acggggaaca ttatgatgcc cgtctggaac
     3601 aggcaggctg gtgttcttcc ggattcaaag attacggctg ggaaaacgca gttctgaaaa
     3661 aggcccctga aggtaaaatg atagctcata cggcctgtcc tgaccgtgtt atggaagtgc
     3721 ttaaaccagt taaaacggaa aaaaccgggg agggtaacta caggattgat ttcgggcagg
     3781 agatatcagg ctgggtccat ctgaagaata tgaacggtga agccggcagg aagattgaaa
     3841 tcagatatat tcaccgggtt ccgtcgggag aaaacagcta tatcatgaaa ggcagcggag
     3901 atgaatccta tcatgcacga tttaactggt ttgtgttccg ggaggtggaa atcctgaact
     3961 ggccgggaga actgaaagac ggacaggtta cagccgaggc agtctatacc cggattgaga
     4021 caagcggaaa atttaaatgc tccgccggac tgcttgaaga cattaacagg atatggtgga
     4081 gaagccagac cgacaatatg cacgggggaa tcgcaagtga ctgcccgaac agggaaagat
     4141 caccatatac cggcgatggc caggcagcct gcgttaccgt aatacacaat ttcgatgcta
     4201 gggcatttta caacaaatgg atacatgata tcatcggtgc atccaatccc caaaccggct
     4261 atgtgccgaa tggtgctcca tggcagcccg gatgcggcgg tggtgttgca tggggggcag
     4321 ccatctgcat aatgccctgg gagttctggg ttcactatgg agacagggat attctccggg
     4381 acagctatga ggccatgaaa ggttatgtga aatacatgct ttcatggaca gatgaaaaag
     4441 gcatcatgtt ttcgcaggcc gtgggcaggg acggcaaagt ccttcgctgg atgaacctgg
     4501 gagactggat ggccccgtat aaactgccgc ctgacaacat ggtgcatacg ttttacctgt
     4561 ggtactgtgc agatattatt tcaagggttg ccgggatact cggtaatcac caggatgaag
     4621 ctatatacgg gaatctggcg gaaaagacaa gaactgcatt catgtcggaa ttttatgatc
     4681 aggtgaatgg tacttttggc agatacggag gcaatataat tgcccttaaa atgggagttc
     4741 ctgaagagat aaagaaaaaa gtaataaagg tcttggccga tgatattaaa gcagacaaag
     4801 gtaatctgga tacaggaatt ttcggcacca ggttcttttt tgaggtgtta tcggcgaacg
     4861 gactgcatga cctggccttt gaagctatga ataagcggac tatgccctca tacggttact
     4921 ggcttgaaca gggtgcgacg acaatgtggg agcagtggga ccagcccctc tcaggcaatc
     4981 atccgatgtt cggcggcgga attgtatggc tgtacagaaa tcttgcagga atgaatgccg
     5041 atcccgcaga cccgggttac cggagaatag tgttctgccc cggaccggt
//
LOCUS       DAOSPX010000238         5073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_14993_length_5073_cov_4.371064, whole genome shotgun sequence.
ACCESSION   DAOSPX010000238 DAOSPX010000000
VERSION     DAOSPX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14993_length_5073_cov_4.371064"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLZ75_10670"
     CDS             <1..187
                     /locus_tag="PLZ75_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOS72894.1"
                     /translation="GCAVIISHDRWFLDRVATHMLAFEGDSKVVWFEGSYSEYEENYR
                     KRVPDSSPKRIRYKKLA"
     gene            complement(220..3051)
                     /locus_tag="PLZ75_10675"
     CDS             complement(220..3051)
                     /locus_tag="PLZ75_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS72895.1"
                     /translation="MIHLQNITAAAVKNHILTVLFLALSLTVSSQTKRTLTIDDLSEW
                     NRIRENIISDDGSFCAFVYEPWDGDPVARLFDGQGSEKATFPYSSGIRFTSDSRFLIF
                     TITTPKEEIKKLKLKKVKKDDMPVNSLGIYDISAGSTDTIYRIKSYKLPAKWPGWLAY
                     QCEPQKKGIVPADRQDSDTSSTERKKTVQESEDNGYHLIIRNLTDKSSDTVRFVTSYV
                     FAEEAPVLVCSTSGGEDETAAGVLVIDLERNRRSEIFNGKGRFRQLTVAKDGKRAAFL
                     MTADMKDKTGNNYSLFWWKGSGNALLAANQGTPGIPEKWIISENGQLVFAEKSQRLFF
                     GTSPENRIRDTTILDEDRPNVDVWHYAEGKLHTVQVIDRERDMKKTYTAVFHCDRKTV
                     VQLATPEMPDTRLIDKGDSPKILASSNLPYQLRSMWEGGNTRNDYWLVDVFTGMPQKI
                     KENYRARIQPSPAGRYLYWYHGPDSSWYSYSISEGKEYRLTDPSTLPCHNELNDVPDL
                     PPSYPPAGWLKDDRAFLISDRYDIWMLDPAEKNPPANITINGREKKITYSLLRLDTEN
                     DYIDPAELQYLAAFNEKTKGSAFYSLSLKKKNEPLFLSGGDYLTGRPLKAGKGSRVIY
                     TIEDFSRFPDYYLSDLSFRGETKVTDANPRQEEFLWGTAELVSWTSLDGKETEGLLYK
                     PEGFDPSRKYPMIVNFYEKSSSGLFSHRIPEPGRSTIDYHYYTSNGYLVFNPDVHYQD
                     GYPGQSAFNCVIPGIMALIEKGFVDEKRIGAQGHSWGGYQVAYLATRTNLFAAIESGA
                     PVVNMYSAYGGIRWETGLNRSFQYEHQQSRIGATIWEAPHTYWENSPLFFTDKVNTPI
                     LIMANDKDGHVPWYQGIEYFIALRRLGKPAWLLNYSGEPHWPLKHPNKVDFQKRMSQF
                     FAHYLKGEPMPVWMKEGVPATEKDFTSGY"
     gene            complement(3215..3436)
                     /locus_tag="PLZ75_10680"
     CDS             complement(3215..3436)
                     /locus_tag="PLZ75_10680"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOS72896.1"
                     /translation="MQQTGKILIITGIILIIAGLILYFAGNKLSWLGHLPGDIRVEKE
                     NMRFYFPLTTMILVSVLLSLILYLLRKFF"
     gene            3694..3930
                     /locus_tag="PLZ75_10685"
     CDS             3694..3930
                     /locus_tag="PLZ75_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72897.1"
                     /translation="MRKLFFSLLPAVLTLHLSLAGQQLTVSDQIRYDRVVREQDLQSD
                     GQALHPAAARAIARARSPEASGTSAMSWFQMPGC"
     gene            3924..>5073
                     /locus_tag="PLZ75_10690"
     CDS             3924..>5073
                     /locus_tag="PLZ75_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72898.1"
                     /translation="MLTPLQKSSLKLEQDGILDLVRGAFRPSPDSVYIDPASNLVLQA
                     APVDDSRMLALRPPLSAVFADIDIPEQEVPVTLANTTSLARDVLESSLQINNKYAVNL
                     RFDSVSFLIDKVDDDSLVVTLVGQILLANPRVEGKYSKNSGYRLVFLASEQVDMKIYT
                     SMRLKEEVKTPIWGTEISISDLGKCEVGLFLLTSVEGHVTLAVEIHQGIEMALGAKGG
                     TFWYIPTSLNNISAFDSWCDVKYKISSEMKAFAGFQCVAKLSVKGYTALDVFVNGGME
                     GTVATDGMTLNADVGLRLRAGGKIVSKKFTLLDNYYSLWKYQKPDYQGYSMLIHEACA
                     WGDYVAGEIHRLVPGKAGGAMDTVPYQGNLTVTVYRAGSARENYSATTG"
BASE COUNT         1237 a         1195 c         1239 g         1402 t
ORIGIN      
        1 cggctgcgcg gtaataatct cacacgacag atggttcctc gaccgtgtgg caacccatat
       61 gcttgccttt gaaggagatt caaaggtggt ctggttcgag ggcagctatt cggagtatga
      121 ggaaaactac aggaaaaggg ttccggattc aagcccgaaa aggatcaggt acaagaagct
      181 tgcgtgactc ctttcttttc ctttattcac acaggcctgt cagtaaccgc ttgtaaaatc
      241 cttttccgtt gccggtactc cctccttcat ccacacaggc atcggctccc ctttcagata
      301 gtgggcaaag aactgtgaca tccgtttctg gaaatcaacc ttgttaggat gtttcagggg
      361 ccagtggggt tcaccgctgt agttaagaag ccatgccggc ttgcccagtc tccggagtgc
      421 tatgaagtat tcaatcccct gataccaggg aacgtgtccg tccttgtcat ttgccattat
      481 cagtataggc gtgttcacct tgtccgtgaa aaacaatggc gaattttccc agtaggtatg
      541 gggtgcctcc cagattgttg cgccgatacg gctttgctgg tgctcatact ggaacgacct
      601 gttcaggcct gtttcccacc ttattccccc gtaggcgctg tacatgttga ccacgggagc
      661 acctgattct attgctgcaa acaggttggt gcgggttgcc agataggcaa cctggtatcc
      721 gccccatgaa tgaccttgtg cccctatgcg tttctcatca acaaaacctt tttcaatcag
      781 tgccattatc cccgggatca cacaattaaa tgcactctga ccggggtagc cgtcctggta
      841 atggacatcc ggattgaaaa caagataacc gttgctggta tagtaatgat agtcaatggt
      901 ggaacgtccg ggttcaggga tgcggtgact gaagagtccg gaggagctct tttcatagaa
      961 attgacaatc attggatatt tccgtgaagg gtcgaagcct tccggcttat acagcaggcc
     1021 ttcagtctcc ttgccgtcga gggaagtcca gctgaccagc tcagccgtgc cccagagaaa
     1081 ttcttcctga cggggattgg catctgtaac ttttgtttca cccctgaaag acaggtccga
     1141 caggtaataa tcgggaaacc tgctgaaatc ttcgatggta tagatcaccc gggacccttt
     1201 tccggctttc agcggcctgc cggtgagata atcacctccg ctgaggaaca gcggttcatt
     1261 ctttttcttc aggctgaggg aataaaaagc gctgcccttg gttttttcat tgaaggcagc
     1321 aagatactgc agctctgccg ggtcaatata atcattttca gtatcgaggc gaagaaggct
     1381 gtaagtgatt ttcttttccc ttccgttgat ggtaatgttt gccggaggat tcttttccgc
     1441 aggatcaagc atccagatat catacctgtc ggatatcagg aaagccctgt catctttcag
     1501 ccatcccgca ggcggatatg aggggggcag gtcgggaacg tcattgagct cattatggca
     1561 gggaagagtt gacggatctg taagcctgta ttccttcccc tctgaaatac tgtaggaata
     1621 ccatgaactg tccggaccgt gataccagta aagatatctg cctgccggcg acggctgtat
     1681 cctggccctg taattttcct tgatcttctg tggcatgccg gtaaagacat ccacaagcca
     1741 gtaatcattt ctagtattcc cgccttccca catcgaccgg agctgatagg gcagatttga
     1801 tgaggccagt attttcgggg aatcaccctt gtcaatgagc cgggtatccg gcatttccgg
     1861 agtggcgagc tgaacaaccg ttttcctgtc gcagtggaat acggcagtat aggttttttt
     1921 catatcgcgt tccctgtcaa tgacctgaac ggtatgaagc tttccttcgg cataatgcca
     1981 cacatccaca ttgggccggt cctcgtcgag tattgtggta tcccggatcc tgttttccgg
     2041 tgaagtgccg aagaagaggc gctggctttt ctctgcgaaa acaagctgtc cgttttcgct
     2101 gatgatccat ttttccggaa tccccggtgt tccctggttt gcagcaagaa gtgcatttcc
     2161 tgatcccttc caccagaaaa gggaataatt atttccggtt ttatccttca tgtctgccgt
     2221 catcaggaaa gcagccctct taccgtcctt tgcaactgta agctgcctga acctgccctt
     2281 tccgttgaat atctccgagc gcctgttcct ttccaggtca ataaccagga ctcctgcagc
     2341 tgtttcatcc tccccgcctg atgttgaaca tacaagtacc ggggcctcct cggcaaagac
     2401 ataggatgtg acgaatctga cagtatcaga agatttatca gtcagatttc tgatgatcag
     2461 gtgatagcca ttgtcttccg actcctggac tgtttttttc ctttcagtgg aagaagtatc
     2521 ggaatcctgc ctgtcggcag ggacaattcc ttttttctga ggttcgcact gatatgcaag
     2581 ccatcccggc cattttgccg gcagtttata tgattttata cggtatatgg tatctgtgga
     2641 accggcggaa atatcatata tcccgaggct gtttaccggc atatcgtcct ttttgacctt
     2701 cttcagttta agttttttaa tttcttcttt cggcgttgtt attgtgaaga tcaaaaagcg
     2761 ggagtcagaa gtgaacctga ttcctgatga ataaggaaat gttgcctttt cagatccctg
     2821 tccgtcaaaa agccttgcaa ccggatctcc gtcccatggt tcgtaaacaa atgcgcaaaa
     2881 tgatccgtcg tcggaaataa tgttttccct tatcctgttc cattcggaaa ggtcatcaat
     2941 ggtcagggtt ctttttgttt gggaagatac tgtcagactg agagccagga aaagtactgt
     3001 cagaatatgg tttttcacag cagctgcagt aatgttttgt aaatgaatca tcttatccgg
     3061 atattttaaa gtgttgtttt ggaaatcatt gtcattctgt ttttttgccg ggcctcggtt
     3121 tctgcaaagc cgtcaaggta taaaattccc tcagcaatat tgcataaaac aaccggtatc
     3181 tcaggttata aaccttgaaa attcattccg gcgcttaaaa aaatttccgt aacagataca
     3241 ggatcagtga caggagaaca cttacgagaa tcattgttgt aagcggaaaa taaaacctca
     3301 tattctcctt ttcaactctt atgtcgcccg gaagatgtcc cagccagctc agtttgtttc
     3361 ctgcaaaata caatatcagg cccgcaataa tcagtattat acctgtgatg atcagaatct
     3421 tacccgtttg ctgcattttc agtttttacg aaagggttta atttacaaaa agttgttctg
     3481 attctgatca ggttttacaa attattaaat accagggcta tgttgccagg ctcccggtat
     3541 tcttatctgc aaattattgg tccagagctg attttgtaca gcatgagagt gtcagatatc
     3601 gaaaatttta tttattttgt aatgaatttg cttctcaggc aaataaagtc aatgattaac
     3661 tgcatcgtag aaaaacaaaa aattttcaga attatgagaa aacttttctt ttctcttctc
     3721 cctgctgtac tgacactgca cttaagcctt gccggtcagc aattaactgt cagtgatcag
     3781 atccgctatg acagggttgt aagggaacag gaccttcagt ctgacggtca ggctttgcat
     3841 ccggctgcag cccgggcaat tgcccgggcg agaagcccgg aagcttcagg aacgtccgct
     3901 atgagctggt ttcaaatgcc aggatgctga cacctttaca gaaaagcagc ctgaaactgg
     3961 agcaggacgg tattcttgat cttgtgaggg gtgctttcag acccagtcct gattcggtct
     4021 atatagaccc tgccagcaat ctggtgctgc aggcagcacc tgttgatgac agccgaatgc
     4081 tggcactccg ccctccgctg tcagctgtat ttgctgatat cgatatcccg gaacaggaag
     4141 tgccggtgac cctggccaat accacctccc ttgcaaggga tgtgttggag agctccctgc
     4201 aaatcaacaa caagtacgct gtaaacctca ggttcgacag tgtcagcttc ctgatagaca
     4261 aggtggatga cgattcgctg gtggtgaccc tggttggtca gatactgctg gccaatcccc
     4321 gcgttgaggg gaaatactcc aaaaacagtg gctacaggct tgttttcctg gcttcggagc
     4381 aggttgacat gaaaatctac acgagtatga ggctgaagga agaagtcaaa acgccgatat
     4441 ggggcacgga gattagtatt tcagacctgg gcaagtgtga ggtcggactc ttccttctca
     4501 cctccgtcga gggtcatgtt accctggctg tggagataca tcaggggata gagatggccc
     4561 tgggggcaaa gggaggaaca ttctggtaca ttcctacaag cctgaataat atttcggcct
     4621 ttgattcatg gtgcgacgtg aagtacaaaa taagttccga gatgaaagct tttgcgggtt
     4681 tccagtgcgt agccaagctc agtgtaaagg gatacactgc actggatgtt tttgtcaacg
     4741 gcggcatgga aggaacagtc gctactgacg gaatgaccct caatgctgat gttggcctga
     4801 ggctcagggc agggggaaag attgtttcaa aaaagttcac cctgctggat aattattact
     4861 ctctgtggaa atatcagaaa cctgattacc agggttacag tatgcttatc catgaggcat
     4921 gtgcctgggg tgactacgtg gccggtgaga ttcacaggct cgttcccgga aaggccggcg
     4981 gggcaatgga tactgttccc tatcagggca atctcaccgt gacggtatac cgcgccggca
     5041 gtgccaggga aaattacagc gcaacaacgg gca
//
LOCUS       DAOSPX010000239         5068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15008_length_5068_cov_5.068622, whole genome shotgun sequence.
ACCESSION   DAOSPX010000239 DAOSPX010000000
VERSION     DAOSPX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15008_length_5068_cov_5.068622"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1803
                     /locus_tag="PLZ75_10695"
     CDS             76..1803
                     /locus_tag="PLZ75_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOS72899.1"
                     /translation="MKKLIIILIMAFAISGFSQAPQKLSYQAVIRNTDNTLVTNQIIG
                     MRISILKGSASGTSVYMETHTPRSNANGLAVVEIGGGTVISGTFSSIDWSAGPYYLKT
                     ETDPAGGTNYSISGTSQLLSVPYALYSGKAENAATKEDMDRLRQRMINIEELYYGQLP
                     PPSEGLKAFYPFRGNTNDFSGNGWDGTVHGATLTSDRFGKADNAYHFTGTTNITTQYP
                     GVSGNGDRSISFWVRIPAGEAGGSTCFYGSTSNGAFFNPGVFAQAAPHVHLDISNAYL
                     DCAAPNAADGKWHHFVFVFSATLGTTLNGVRVYYDGNLLTGYTGNNFNTYTINTGTAT
                     KFTIGGKTGATSQMSIDDLRFYDRVLDDSEIMLLFYESESGGTAFVNDSDGNSYKVVK
                     IGDQVWMAENLRTTRYNDGTAIPLVTDPTDFINLTTPAYCWSNNDIGNKPVYGALYVW
                     YTVKTGILCPSGWHVPTDAEWTILENHLGGIAVAGGKMKETGTEHWLAPNTDATNESE
                     FNARPGGWRDGLSGGFLPPGQVSMWWTSQETYPDRPYWRELYYNSGTIFPKSGGDPSY
                     AMSIRCVKD"
     gene            complement(2058..4340)
                     /locus_tag="PLZ75_10700"
     CDS             complement(2058..4340)
                     /locus_tag="PLZ75_10700"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOS72900.1"
                     /translation="MNKKIKLPFCVISLIFTVLLAGCGSDTNSPADYVNPFIGTDYHG
                     HTFPGAALPAGMVQLSPDTDVQGWDWCSGYHYSDSSVMGFSHLHRSGMGAGDWGDILL
                     MPATGELKIIPGSKNNPGEGYRSRFSHNEEEAEPGYYAVTLKDYDIRAELTVTPRTGF
                     HRYTFPESGSSHIIIDLKHGIRDSCTNAMVRILNDREIEGFRTSIGFVKSHTVYFCAE
                     FSKPFESYGTWKGRDIKKDSQEESGYNIGAFVNYNTSGDEEIMVKVGISYTSIDQARL
                     NLREENPGWNFNRVKKEAGEKWNRELGRIKAEFITQNDGTWNQDRLVTFYSALYHSLL
                     FPSIFGDSDGKYTGLDEEVHTAKGFTYHSDFSLWDTFRAEMPLLALIKPEIIKDAIRT
                     MLSQYEQGGWLPTPQQFGNCYTNDMIGDHPAASITDAYMKGIRDFDAGKAYEAVRKNA
                     MEKPVGHRSRGRVGLEYYKRFGYIPYDKERESVSRTLEYAYNDWCVAQLAKALGFTDD
                     YKFFMDRSSCYKNLFDPSTGLARPKDSTGKWLDPFNPTFIGQGRERHYTEANAWQYTW
                     FVPHDIDGLISLEGGPQKFIAKLDTLFTMSSEIQSTVSDVTGLIGQYAHGNEPSHHTI
                     YLYDHAGAPWRTQELIRHVMDNLYHSGPDGLCGNEDMGQMSAWFVFSAMGFYPVTPGN
                     NIFSIGSPVFDRITVSLDKKYYKADKFIIETRNNSKENKYIQSVILNGKPLEKPWFDH
                     SEIVKGGTLIFEMGPKPKLW"
     gene            complement(4455..>5068)
                     /locus_tag="PLZ75_10705"
     CDS             complement(4455..>5068)
                     /locus_tag="PLZ75_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS72901.1"
                     /translation="PFQMAKQAVPVDILVKHVYQDAESVIRGMENITLSIMNFVHAAV
                     RLGVDGFYTCTQGGETNRIADRQLFNKTVKAYDMILYKNVSELTSFNILHICDYEGTY
                     EEFESRFRDYPGQVVNVPLEADHKPLSLKLASEIFRRPVMGGIDRLGVISSGSAEDAR
                     NAAIKVLKDAPDNFILGADCTVDSKTPVANLRAAIDAAHNFRK"
BASE COUNT         1376 a         1134 c         1109 g         1449 t
ORIGIN      
        1 ttttatttgt tccgattttt ttttcttccg aaatttggtt atacatatac tcatttacag
       61 gcaaatatta atcccatgaa aaagcttatt attatcctga taatggcttt tgccatatca
      121 ggcttttctc aggcgcctca gaaactaagt taccaggcag ttatccgtaa taccgataat
      181 acacttgtta caaaccagat cattggaatg agaatcagta ttttaaaagg ttctgcctcc
      241 ggaacatcag tttacatgga aacacatact ccccgctcaa atgcaaacgg acttgctgta
      301 gtggaaattg gaggtggaac agttatttcc ggaacatttt catcaataga ctggtctgcc
      361 ggtccttatt accttaagac cgaaacagat cctgcgggtg ggacaaatta ttccatttca
      421 ggaacaagtc agctgttgag tgttccttat gctttatatt ccggtaaagc agaaaatgcg
      481 gcaactaaag aagatatgga cagattgcgg cagaggatga taaatattga agaattgtat
      541 tatggtcagc ttcctccgcc ttcagaaggt ctgaaagctt tttacccttt cagggggaat
      601 accaacgatt tcagcgggaa cggctgggac ggaacggtcc acggtgccac gctgaccagc
      661 gacaggttcg ggaaagcgga taatgcttat catttcaccg gaaccacaaa tattaccact
      721 cagtaccccg gagtctccgg gaacggagac cgctcaattt cattctgggt aaggatccct
      781 gccggtgaag ccggaggaag cacctgcttc tacggatcaa cctcgaacgg agcattcttt
      841 aatccggggg tttttgccca ggcggccccg catgttcatc ttgatatcag taatgcatat
      901 ctggattgtg ctgcaccaaa tgcagccgac gggaaatggc atcactttgt ttttgttttt
      961 tctgccactc ttggtacaac ccttaacgga gtcagggttt attatgacgg aaatcttctt
     1021 acaggataca cgggtaacaa ttttaacaca tatacgatca atacaggtac agccacaaaa
     1081 tttacgatcg gagggaaaac cggggcaaca tcacagatgt caattgatga tcttagattt
     1141 tatgaccggg ttctggacga ttccgagata atgctgcttt tttacgaaag tgaatccggc
     1201 gggacagctt ttgtcaacga ttctgacgga aattcctata aggttgttaa gatcggcgat
     1261 caggtatgga tggcagaaaa cctcaggaca acgaggtata atgacggaac ggctatacct
     1321 ttggttacag atcccacaga ctttataaac ctgacaacac ctgcatattg ctggagcaat
     1381 aatgatatag ggaataaacc ggtttacggg gcattatatg tctggtatac cgtcaagacc
     1441 ggtatactgt gtccttccgg atggcatgta cccacagatg ctgaatggac aatacttgag
     1501 aatcatcttg gaggtattgc tgttgccggg ggtaaaatga aggaaaccgg gactgagcac
     1561 tggcttgctc ccaatactga tgcaaccaat gagtcagaat ttaatgcacg ccccggaggc
     1621 tggcgtgatg gtctgagcgg aggattttta cctcccggac aagtcagtat gtggtggaca
     1681 tctcaggaaa cttaccctga caggccatac tggcgtgaat tatactacaa ttcaggaaca
     1741 atatttccaa aaagcggtgg tgatccaagc tacgcaatgt cgatacggtg cgtaaaagac
     1801 tgagtgatta ggatatattg ccgaagaatt tgaagaggca ggattaaaaa acctgctggt
     1861 ttatgagaac gggaaacctg tttcactgag gtatgatctg ataggtgttt acaaccttga
     1921 aatcattaaa gaacttaaga aacatattga atatccgacc tggctgatct cagaaaagac
     1981 tttgataatt ttaaaggaga caagagatcc cgtgaatctg aatcttcacg aaaatgatca
     2041 tccggatcac ctgtcagtta ccacaatttt ggtttcggcc ccatttcaaa gatcagggta
     2101 ccccctttca cgatctctga atgatcaaac cagggtttct caagtggttt tccattcagg
     2161 atcacagatt gtatatattt gttttccttt gaattgttcc gggtctcaat aatgaatttg
     2221 tcagccttat aatatttctt gtcgagacta actgttattc tgtcaaatac ggggcttcct
     2281 attgaaaaaa tattatttcc gggtgtgacg ggataaaatc ccatagcact gaaaacgaac
     2341 catgcagaca tttgtcccat atcctcattt ccgcaaagac catccggtcc ggaatgataa
     2401 agattatcca tgacatgtct gataagctcc tgagtacgcc agggagctcc ggcgtgatca
     2461 tagagataaa ttgtatgatg ggaaggttca tttccatgag catactgtcc gatcaatcct
     2521 gtgacatctg atacggtact ctgaatttcg gagctcattg taaagagggt atcgagtttg
     2581 gcgatgaatt tctgaggtcc gccttccagg cttatcagtc cgtctatatc atgaggtaca
     2641 aaccaggtat attgccatgc attggcttcg gtataatggc gttcccttcc ctgtccgatg
     2701 aatgtgggat taaaaggatc aagccatttg ccggtactgt ccttcggacg ggccagacct
     2761 gttgaaggat caaagaggtt tttgtagcag gaagaccgat ccataaagaa tttgtaatca
     2821 tcagtaaagc cgagagcctt tgccagttgc gcgacacacc agtcgttata ggcatattcc
     2881 agtgtacggg aaaccgattc tctttccttg tcatagggaa tgtatccgaa tcttttataa
     2941 tactccagtc ccacacggcc tcttgacctg tgtccgacag gtttttccat tgcattttta
     3001 cggacggctt cataagcttt tccggcatcg aaatcgcgga tacctttcat atacgcatct
     3061 gtgatagatg ctgccggatg atcgcctatc atatcatttg tgtagcaatt gccaaactgc
     3121 tggggagtcg gaagccaccc gccctgttca tactgtgata acattgtccg gatcgcgtct
     3181 ttaatgatct caggttttat aagcgccaga aggggcattt cggccctgaa tgtatcccag
     3241 agggagaagt cgctgtgata ggtaaaacct ttggctgtat gaacctcttc gtccagtccg
     3301 gtgtattttc cgtcagagtc cccgaaaatt gaagggaaca atagtgaatg gtacagagcg
     3361 gaataaaatg tgaccagtct gtcctggttc catgtcccgt cattctgtgt gatgaattca
     3421 gctttgatcc tgcccagttc cctgttccat ttctcacctg cttctttttt tacacggtta
     3481 aaattccagc cgggattctc ctctcgtaga ttcaggcgcg cctgatcgat acttgtatat
     3541 gatattccga ctttaaccat gatctcttca tcaccagaag tgttataatt gacaaaggcc
     3601 ccgatattat aaccggattc ttcctgtgaa tcctttttta tatcccggcc tttccatgtt
     3661 ccatatgatt caaatggttt actgaactct gcacagaaat atacagtatg gctctttaca
     3721 aatccgattg atgttctgaa gccttcaatt tccctgtcat ttaatattct taccattgca
     3781 ttcgtacagg aatcccttat accatgtttc aggtcgatga ttatatgtga ggatccggat
     3841 tccgggaaag tatatctgtg aaacccggtc cttggtgtga cagttaattc tgccctgata
     3901 tcataatctt ttaatgttac ggcataatat cccggttctg cttcttcttc attatgggaa
     3961 aaacgtgatc tgtatccttc gccgggattg tttttactgc cgggaattat tttaagttct
     4021 cctgtagccg gcattaaaag aatatcaccc cagtcccccg cgcccattcc gctcctgtga
     4081 agatggctga atcccataac ggaactgtca ctgtagtgat agcccgagca ccagtcccag
     4141 ccctgaacat cagtatcagg acttaactgg accatacctg caggtaaagc tgctcccggg
     4201 aatgtatgac catgataatc cgttccgata aagggattta cataatcagc cggactgttg
     4261 gtatctgaac cacatccggc aagtaagact gtaaagatta acgatataac acaaaacgga
     4321 agttttattt tcttattcat tttcaagatt tttgatacgt cctatcaaca atgaaatcac
     4381 cagtaacact cctgcaatag cggcagcaac caccaggctc tgctgcacag gaaataatgc
     4441 agatgcggat caaatcattt tctgaaattg tgagctgcat ctattgctgc cctgagattg
     4501 gccacggggg ttttgctgtc gactgtgcaa tccgctccca ggatgaaatt atccggcgca
     4561 tccttaagta ccttaattgc agcatttctg gcatcttctg ccgaaccgga agaaataacc
     4621 ccgagcctgt caatcccgcc cattaccgga cgcctgaaga tttccgatgc aagcttgagg
     4681 gataaaggtt tgtgatctgc ttccagcggg acattgacaa cctgtccggg ataatcccgg
     4741 aatctggatt caaattcttc atatgttccc tcatagtcac aaatgtgcaa tatgttgaaa
     4801 gaagtcagtt ccgatacatt tttatataga atcatatcat atgccttcac tgtcttattg
     4861 aaaagctgcc tgtccgctat ccggttggtt tcacctccct gggtacaggt atagaagccg
     4921 tcaaccccga gacgcaccgc ggcatgcaca aaattcatga ttgacagtgt aatgttttcc
     4981 atgcctctga ttactgattc tgcatcctgg taaacatgtt ttacaagaat atcaacaggc
     5041 acagcctgtt tggccatctg gaaaggtg
//
LOCUS       DAOSPX010000240         5066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15015_length_5066_cov_4.531032, whole genome shotgun sequence.
ACCESSION   DAOSPX010000240 DAOSPX010000000
VERSION     DAOSPX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15015_length_5066_cov_4.531032"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="PLZ75_10710"
     CDS             <1..1591
                     /locus_tag="PLZ75_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS72902.1"
                     /translation="NVDIKGYTWLMENTLTYKLAFDAHHLNLLAGQTVQKYVSESAQI
                     AVERFADERLGYYNLGLGLDKTVYSGYNSWAMLSGLTRVMYDYDNKYYLTFSGRLDGS
                     SKFGSYNRYGFFPSVSTAWRITREPFFENIKVINDLKLRLSAGSVGNEGIPSGSTLSL
                     MGTRQYFYGEGSGAKVIGTYVYSLQNNDLKWEVTRQYDIGIDLSLFNSRVEFTAETYL
                     KNTSDLLLYLPVNRSSGFEYVWANVGNLRNHGYEFSVNSVNAVNKFKWDTRFNLAFNK
                     NKVTNLEKSDNIYGTSVLNILDWTMISEGKPIGMIYGYKSDGIIQLGEELSDIPFFPA
                     KIPRYGDRKYVDRNKDGKLTVDDYFELGNANPDFSFGLKNTFSYANFSLDIYIQGDYG
                     NEIVNFNRFQLESFDGYQNNLAVALERWTESNPSSRYPRANASSHGIIMSDVYVEDGS
                     YARLKEVILSYSLPQSLLGKMKIRNMRLSLSGSNLLTLTRYSGYDPEVSIFGGNVFGK
                     GADYGAYPMARTLLFSINATF"
     gene            1610..3076
                     /locus_tag="PLZ75_10715"
     CDS             1610..3076
                     /locus_tag="PLZ75_10715"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS72903.1"
                     /translation="MKKITVFIIVALLISSGCKDFLEQDLRSDLNYNNYFQSEKDLIT
                     FADGMFGGLITWTWTGGGLFFNNYWVVQDLASDNCFETGPSIDMHDLSQFTFDPYNST
                     LYYIWVGCYEVIQSANVLLAESENIKTYSRPEIKNHLQGEAYFLRGMLYFELVRLFGE
                     VPLQLFPTKSIRETQMERNPVSEVYESIVSDLKKAEQLLAVNPFLKRVEGMPTSHTVS
                     ALLAKVYLTMAAAGGDASYYTSAKASLEKIIGHYSLEPEFADIFKISNANSGEILWAV
                     NFSGTLGEGWTTNQFMVRLMPTTISPRGVRNGQGWERPLDYLYHSFSDEDHRKAATFI
                     TGFEDEVFNGPYIRKYWDQEAEGGRQNGESDADFIYMRYADVLLMYAEVLNEINNGPD
                     DNAYRSINDVRNRAGLADLPDGLDYQLFKDALLQERQWEFVMEGHRWYDLVRMGKLVE
                     CVQRAKPTANVKSHHILFPVPQKERYLNPKLTQNEGYY"
     gene            3060..4844
                     /locus_tag="PLZ75_10720"
     CDS             3060..4844
                     /locus_tag="PLZ75_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOS72904.1"
                     /translation="MRDITNRNTVTEPNFNQHPAVPVREYNDGYSGGWNEISGTLSEQ
                     LEENNSGRKVIVVDCYQGVLFEELLSLFSDMMKPVLTITSRDYFLPPEEISRITWPDV
                     TDDRVFGYVTRLTIKDFISPGKLDECRSRIDSVTGGTVLVFGYGAALAFSSPDLLIYA
                     DMPRREIVMRMRSGRVNNLGISNKDELFEQQYKRGFFVDWRVCDRHKKKLKDKWSFVL
                     DSADMSNPAMIRADCYDGALTKAVSVPFSVVPTFDPGLWGGQWMKEKLGLDPSSPNYA
                     WCFNCIVEENSLLFDFGRGLFETPASNLVYFRPVELLGEAVYGRFGDEFPIRFDYLDT
                     IEGGNLSLQVHPVTEYIQEKFGMHYTQDESYYIVDTGEDASVYLGLREGISPAEMIED
                     LLDAQNGNQPFDDTRYVNRWPAEKHDHFLIPGGTVHCSGKNCLVLEISSTTFVFTFKL
                     WDWGRIDSDGRPRPINIWHGKEVIQWERDTEYVRNNLINRIRTVAEGVGWKEESTGLH
                     VREFIETRRHWFTRKVTHNTNGGVNVLDLVEGREVIVESPEGRFEPFIVHYAEVFIVP
                     AAIGEYTIRPYGEAEGTECVTIKAYVRT"
     gene            4861..>5066
                     /locus_tag="PLZ75_10725"
     CDS             4861..>5066
                     /locus_tag="PLZ75_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72905.1"
                     /translation="MRKEICLMIFLLLMAATACDKKQESGKIADNIPDLEELSGIWVS
                     CDTVEMEPSIRNFRGQALLNRDMTS"
BASE COUNT         1437 a         1032 c         1272 g         1325 t
ORIGIN      
        1 aaatgtggat atcaagggat atacctggct gatggaaaat accctgacct ataaactggc
       61 cttcgatgct caccatctta acctgctggc cggccagact gttcagaaat atgtatctga
      121 atccgcacag attgccgttg aacgttttgc tgacgaacgg ctgggatatt ataatcttgg
      181 cctcggactg gacaaaacag tgtattccgg ttacaactcc tgggcaatgc tgtccgggct
      241 gacaagggtt atgtatgatt atgacaacaa atattacctg accttttcag gaagactgga
      301 cggatcatcc aaattcggaa gctataacag atacggattt tttccgtctg tttccacggc
      361 ttggaggatt accagggaac ctttcttcga aaatataaaa gtaatcaatg acctgaaact
      421 gaggttaagt gcaggaagcg tgggaaatga agggattcct tcgggaagta ctctcagcct
      481 tatgggaacc aggcagtatt tttacggtga gggaagcggt gcaaaggtaa taggcacata
      541 tgtctattcg cttcagaata atgatctgaa atgggaagta accagacagt acgatattgg
      601 catcgatctg agccttttca attcacgtgt cgaatttacc gctgaaacct atctcaagaa
      661 cacatcggac cttctgcttt atcttccggt aaaccggtcc tcgggattcg aatatgtatg
      721 ggcaaacgtc ggcaatctcc gcaaccatgg ctatgagttc tctgtcaaca gcgtcaatgc
      781 agtgaataaa ttcaaatggg atacccgctt caatctggcc tttaacaaga ataaggtgac
      841 aaatcttgaa aagtcagaca atatctatgg cacttcagtc ctgaatattc ttgactggac
      901 catgatcagc gaaggtaaac ccatcggaat gatatatggt tacaagtcag acgggataat
      961 acaactcggt gaagaacttt cggatatacc tttctttcct gccaagatcc cgcgctacgg
     1021 cgacaggaaa tatgttgaca ggaacaagga cgggaaactg accgtggatg attattttga
     1081 actgggcaat gccaatcccg atttttcctt cggtttaaaa aacacttttt catatgccaa
     1141 tttttcgctc gatatttata tacagggcga ttacggtaat gagatagtaa atttcaaccg
     1201 gtttcagctt gagagctttg acggttatca gaacaatctt gcagttgccc ttgaaaggtg
     1261 gaccgaaagc aatccctcct ccaggtatcc cagggccaat gcatcatccc atggtataat
     1321 aatgtcagat gtttatgtgg aagatggatc ctatgcaagg ctcaaggaag tgattctttc
     1381 ctacagcctt ccgcaatcac ttctcggaaa gatgaagata agaaacatga ggctttccct
     1441 gagcggttca aacctgctga ctcttacccg ttattcggga tatgatccgg aagtgtcaat
     1501 tttcgggggc aacgttttcg ggaaaggagc tgattatgga gcatatccca tggcaagaac
     1561 attgcttttc agtataaatg ccacatttta aaagattgaa ttatttgcaa tgaagaaaat
     1621 tacagtattt ataattgtcg ccctgctgat ttcatcaggc tgcaaggact tcctggaaca
     1681 ggatcttcgt tcagatctga actacaataa ttatttccag tctgaaaaag acctgataac
     1741 ctttgctgac ggaatgttcg gcggactgat tacatggacg tggaccgggg gcggactgtt
     1801 ttttaacaat tactgggtgg ttcaggatct tgcttccgac aactgctttg aaacaggtcc
     1861 ctcaattgac atgcatgacc tgtcacaatt cacattcgat ccgtataact ccaccctgta
     1921 ttatatatgg gtcggctgtt atgaggttat tcagtcagcg aatgtgctcc tggcagaatc
     1981 cgagaatatt aaaacctatt caaggccaga aataaagaat catcttcagg gggaagccta
     2041 ttttcttcgc ggcatgctct attttgagct ggtcaggtta ttcggggaag taccccttca
     2101 gctttttccg accaagagta tcagggaaac acagatggag agaaaccctg tttccgaagt
     2161 atatgaaagc atagtcagtg accttaagaa agcagagcag cttctggctg tcaatccttt
     2221 tctcaaaagg gtggaaggta tgcccacaag ccatacagta tcagccctgc tggcaaaagt
     2281 gtatttaacg atggcagccg ccggcggaga tgcttcatat tacacctccg caaaagcatc
     2341 ccttgagaaa attatcggac actattctct ggaaccggaa tttgctgata ttttcaaaat
     2401 aagcaatgcc aattccgggg aaatattatg ggcagtcaat ttcagcggta ccctgggaga
     2461 gggctggaca acaaatcagt ttatggtaag actgatgcca acaacaattt ctccccgcgg
     2521 agtgagaaac ggccagggat gggaaagacc gcttgattat ctttaccaca gcttcagtga
     2581 tgaggatcac aggaaagcgg caacattcat aaccggattt gaagacgagg ttttcaacgg
     2641 gccttatatc aggaaatact gggaccagga ggctgaggga ggaagacaga acggggaatc
     2701 cgatgctgat tttatctata tgaggtatgc ggatgtattg ctgatgtatg cagaggtact
     2761 gaacgagatc aataatggtc cggatgataa tgcatacagg tccattaatg atgtgcgtaa
     2821 cagggcagga ctggcagatc ttcctgacgg acttgattat cagttgttta aagatgccct
     2881 gctgcaggaa aggcagtggg aattcgttat ggaaggacat cgctggtatg acctggtacg
     2941 gatggggaaa cttgttgaat gtgttcagag agccaagccc accgccaatg taaaatctca
     3001 tcatattttg tttccggtac cgcagaagga aagatacctt aatcctaaac tgactcagaa
     3061 tgagggatat tactaacaga aacacagtga cagaacctaa cttcaatcag catccggcag
     3121 taccggtaag ggaatacaac gatggctatt caggaggatg gaatgagata tccggaaccc
     3181 tttccgaaca gcttgaggaa aataattccg gccggaaagt gattgtggtg gattgttacc
     3241 agggagtgct ttttgaagag ctgctttccc tgttttcaga tatgatgaaa cctgtactga
     3301 caattaccag cagggattat tttctgccgc cggaagagat ctcacggatt acctggcccg
     3361 atgtaaccga tgaccgtgtt ttcggatatg tcacccgttt gaccataaag gattttatca
     3421 gccccggaaa gcttgatgaa tgcagaagca ggattgattc cgttaccggc ggaacagtcc
     3481 ttgttttcgg ttatggcgcg gcacttgcat tcagcagtcc ggacttgctg atttatgccg
     3541 atatgccccg cagggaaata gtgatgagaa tgagatcggg cagggtgaac aatctgggta
     3601 tcagtaataa ggatgaactt tttgaacaac agtacaaaag gggatttttt gtagactggc
     3661 gggtgtgcga caggcacaag aagaagctta aggacaaatg gagctttgtt cttgattccg
     3721 ctgacatgag caatccggca atgataaggg ctgattgcta tgacggggca ttgacaaagg
     3781 cagtatcggt ccccttcagt gttgttccca cctttgaccc gggattatgg ggcgggcagt
     3841 ggatgaagga aaagctcggc cttgaccctt cctctcccaa ttatgcatgg tgttttaact
     3901 gcatcgtgga ggaaaacagc ctgttgtttg atttcggcag gggattgttt gagactcccg
     3961 catccaacct ggtatatttt cgtcctgtcg aactgctggg agaagccgtt tacgggcgtt
     4021 tcggggatga gtttccgata aggttcgatt accttgatac cattgaaggt ggaaatctaa
     4081 gccttcaggt acatcctgtt acggaataca tccaggaaaa attcggcatg cactataccc
     4141 aggatgaaag ttactatatt gttgatacag gcgaagatgc ctcggtatac ctgggcctca
     4201 gggaaggtat cagtcctgca gaaatgattg aggatctcct ggatgcacag aacggaaatc
     4261 agccttttga cgataccagg tatgtaaaca ggtggcctgc tgaaaagcat gatcatttcc
     4321 ttattcccgg aggtactgtt cactgttcgg gaaagaactg ccttgtcctg gagattagtt
     4381 caacaacctt tgttttcaca ttcaagctgt gggactgggg acgtattgat tcagacggaa
     4441 ggcccaggcc cataaatatc tggcacggga aggaggtgat acagtgggag agggatactg
     4501 agtatgtacg caacaatctg ataaacagga tcaggactgt tgctgagggt gtgggatgga
     4561 aagaggaatc cacaggattg catgtgaggg agtttattga aacacgcagg cactggttta
     4621 ccaggaaggt tacccacaat acaaacggcg gagtgaatgt acttgatctg gttgaaggca
     4681 gggaagtgat tgttgaaagt cctgaaggcc gctttgagcc cttcattgtt cattatgccg
     4741 aagtatttat tgttccggct gcaatcgggg aatataccat ccggccttac ggggaagccg
     4801 aaggcacgga atgcgtcaca ataaaagctt atgtaagaac ataataaaac catatatcag
     4861 atgagaaaag aaatatgcct gatgattttt ctgttgctga tggctgcaac agcttgtgac
     4921 aaaaaacagg aatccgggaa gattgcggat aatatccctg atcttgagga actgtcggga
     4981 atctgggttt catgcgacac tgttgaaatg gaaccttcaa tcaggaattt ccgggggcag
     5041 gccctgctta acagggatat gacttc
//
LOCUS       DAOSPX010000241         5065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15019_length_5065_cov_3.574052, whole genome shotgun sequence.
ACCESSION   DAOSPX010000241 DAOSPX010000000
VERSION     DAOSPX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15019_length_5065_cov_3.574052"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLZ75_10730"
     CDS             complement(<1..390)
                     /locus_tag="PLZ75_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOS72906.1"
                     /translation="MRTNLSAILIAGIIIFLPLSCREKTGSLRLWYDHPADASVPDTG
                     DGWRNDPEWLKALPVGNGFLGAMIFGDVNMERIQLNEKSLWSGSPDDNNNPEAYTSLN
                     AIRQLLFDGKYREATELTLRTQVCRGAG"
     gene            complement(502..1905)
                     /locus_tag="PLZ75_10735"
     CDS             complement(502..1905)
                     /locus_tag="PLZ75_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS72907.1"
                     /translation="MKTLRLSLVIICLAMLKVSAQHDNRYVPDPDPAIRNRIEEWQDI
                     KFGLLMHWGPYSQWGIVESWSICPEDEPWCIPDTVQDYFAYKSRYENLGRTFNPVSFD
                     PDKWAKAAKDAGMKYVVFTTKHHDGFCMFDSKYTDYKITGMDCLFHTNPRADVTKAIF
                     DSFRKEDFWIGAYFSKPDWHCPDYWWPHFPPFDRNPNYDLEKYPGKWNNYVEFTHNQL
                     MELCTGYGKIDIFWFDGGWVKKRNTTAHPYNQDVRMDELVEKIRSKQPGVLVVDRAVS
                     GKNQNYLTPENTVPDRMLPYPWESCIILGGGWSFSYNARMMPERRLIHMLADIVAKGG
                     NLLLNIGPGPDGTWYDEAYDRLRETGEWLRINGNAIYNTRPVAPYTDGKLRFTKGKDG
                     SVYIIYLLDENEKLPSSLRISGLMPQKGARVNMLGRRGNLGWKKEGEGFTISIPASVS
                     DAMPSEYAVAIRISAVL"
     gene            2263..3135
                     /locus_tag="PLZ75_10740"
     CDS             2263..3135
                     /locus_tag="PLZ75_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOS72908.1"
                     /translation="MSSIFTWIILIKAMKEKNLVYSGKSKDVFSISEGQYRGKYLLVF
                     TDKATGYLENGKPVFDPGYDTVVGSIPGKGAIACRFATYFFRLLREKGIATHYIETIS
                     DSEMVVEPALPLSLPIEAPGFPGSAPLENLEFTWRNSATGSFWRRYPFVKPCKNIHKV
                     VEAWTKGDSDTLITFEALEETGAMSRDEISGSIELVKDIAEIVTREFESKGYHVLDGK
                     FELGRLRDGDGKIVLIDEISPDVLRVCKGYSPDHKGDCTIIRDCVMTGISGGKRTIKN
                     RNQVKAADFINIFL"
     gene            3153..3887
                     /locus_tag="PLZ75_10745"
     CDS             3153..3887
                     /locus_tag="PLZ75_10745"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS72909.1"
                     /translation="MKHLYRIMVIFIFIASASPGLWAQQQEMSFGKSVPLKNVTPGTS
                     LKSSALKWINVNTAEYTWSVKKNILVCTGRPIGVMRSEKQYENFILHIEWKHMEPGGN
                     SGVFVWSEAIPDEKSHLPNGVEVQMLELEWVKLNTKDGIEPPVAYVHGELFGVGGVKT
                     TPDNPRGTRSKSVENRCRGRGEWNYYDVVCVDGVIKLSVNGKFVNGISHSTRKTGYLC
                     LESEGGEIHFRNIRITELPPGVISEE"
     gene            4226..4714
                     /locus_tag="PLZ75_10750"
     CDS             4226..4714
                     /locus_tag="PLZ75_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72910.1"
                     /translation="MKRMILFAVLVLAAGTFSFGTKLIGEGKTYTSLGNYRVEAIDSP
                     VPLKGADCQAYVIKYENTPMEVTVIVCRDQKDQKYLVLSDRLSVQYVNNAQYFGVEMV
                     DKSFEKEGYKTDNSGLNKIAYFRQKVIGPGQMREKDATRTIAAFFPFLLRTDENMTAE
                     KQ"
BASE COUNT         1435 a         1122 c         1127 g         1381 t
ORIGIN      
        1 gcctgctccc ctgcacacct gggttcgcag tgtcaattct gttgcctccc tgtattttcc
       61 atcaaacaat aactgcctga ttgcattcag agaggtatat gcttccggat tgttattatc
      121 atccggactg ccggaccaca ggcttttttc attcagctgt atcctttcca tgttcacatc
      181 gccgaatatc attgccccca ggaacccgtt acccaccggc agggctttca gccattcagg
      241 atcattccgc cagccgtccc cggtatccgg tactgatgca tccgcgggat gatcatacca
      301 taatctcagg ctgcctgttt tttctctgca ggaaagcggc aggaaaataa ttattcccgc
      361 gattaagatt gctgacagat tagttctcat tgtaacaaaa tcagagttaa ataaatggta
      421 atgtttgtct taattcctga gggaaacaag tatgttaagc agtaaatcaa ggtattaagc
      481 actaaatcaa acgaaatatc actacagcac cgcgctgatc cggatggcaa cggcatattc
      541 cgacggcatg gcatccgaaa cagaagccgg tatgcttatt gtaaatccct ctccttcctt
      601 tttccatccg agattcccgc gtcttcccag catatttacc cttgcgccct tttgtggcat
      661 taatccgctg atcctgagag aagaaggaag tttttcattt tcatcgagca gatagattat
      721 gtataccgat ccgtctttcc cctttgtgaa tctgagtttt ccgtcagtat atggtgctac
      781 gggcctggtg ttataaatgg catttccgtt gatacggagc cattcaccgg tttccctgag
      841 cctgtcatat gcttcatcat accatgtacc gtcaggaccg ggacctatgt tgagcagcag
      901 gttgcccccc ttggccacta tgtcggcaag catgtgaata agtctgcgtt cgggcatcat
      961 tctggcatta taggaaaatg accatcctcc gccaaggatt atacatgatt cccagggata
     1021 gggcagcatt ctgtcgggca ctgtattttc aggtgtaaga taattctggt ttttcccgga
     1081 aacagcacgg tctactacaa gtactccggg ctgctttgac cgtatctttt ctacaagctc
     1141 atccattctt acatcctgat tataaggatg agccgttgta tttctctttt tgacccaacc
     1201 accgtcaaac cagaaaatat ctattttccc gtaacccgta cacaattcca tcaactgatt
     1261 atgagtaaat tccacataat tattccattt gccgggatat ttttcaagat cataattggg
     1321 gttcctgtca aatggcggaa agtgtggcca ccagtaatcc ggacagtgcc agtcaggttt
     1381 tgagaaatat gcccctatcc agaaatcctc tttcctgaat gaatcaaata ttgcctttgt
     1441 tacatcggcc ctgggatttg tatgaaagag acaatccatg cccgttattt tataatcggt
     1501 gtattttgag tcgaacatac agaaaccatc atgatgcttg gttgtgaaaa caacatattt
     1561 cataccggca tcctttgcag cttttgccca tttgtcgggg tcaaagctta caggattgaa
     1621 tgtcctgccc agattctcat acctgctttt atatgcaaaa taatcctgaa ccgtatccgg
     1681 gatgcaccat ggctcatctt cgggacagat ggaccatgat tcaacgattc cccactggct
     1741 gtacggcccc cagtgcataa gaaggccgaa tttaatatcc tgccattcct ctatacggtt
     1801 acggattgcg ggatccggat caggcacata cctgttatca tgctgggctg aaactttaag
     1861 cattgccagg cagataatca ctaaagaaag tctcaaggtt ttcattttac aggcttttgg
     1921 gttatatatc cactccgtcc ccgggcgtgg atttcagtat tgatgaaaat aagcggatat
     1981 aaaaattcat tcatgcctgt caagataata taaatatttt cttttccggt agaaataaat
     2041 ttgaaataac aggcggttcg aatcagcaat gaagtataat agtctgcctc cgttccgtta
     2101 cctgccggat gttcaccgga atctgtgcag actccccgca accgggctta gcaggattca
     2161 gatatattat tgtattttaa attattactg aaacagtaca aaagatgtat agagcaaata
     2221 atatttttcc actatcttct caaatgattt gatgaaatga tcgtgagtag tatatttacc
     2281 tggataattt taataaaagc catgaaggaa aagaatcttg tatacagtgg gaaatcaaaa
     2341 gatgtattca gcatttccga agggcagtac aggggaaaat accttcttgt tttcactgac
     2401 aaggccaccg gttacttaga aaacgggaag cctgttttcg atccgggcta tgatactgtt
     2461 gtcggcagta ttcccgggaa gggtgcgatt gcatgcaggt ttgccacata ttttttcaga
     2521 ctgctcaggg aaaagggtat tgcaactcat tatatagaaa ccatcagtga cagtgagatg
     2581 gttgtggaac cggcactgcc tcttagtctg ccgattgaag ctccagggtt tccgggctcg
     2641 gccccgctgg aaaatcttga attcacctgg aggaacagtg caactggcag tttctggaga
     2701 aggtacccct ttgtaaagcc ctgcaaaaac atccacaagg tggttgaggc atggacgaaa
     2761 ggggacagtg acaccctgat tacgtttgaa gcactcgagg aaacgggagc catgagccgg
     2821 gatgagatca gcggcagtat tgaacttgta aaagacattg ctgaaatcgt tacccgggaa
     2881 tttgaatcaa aaggctatca tgtgctggat ggcaagtttg agctgggcag gctcagggac
     2941 ggtgacggaa aaatagtact cattgatgaa atatctcctg atgtccttcg tgtatgcaag
     3001 ggctacagcc cggaccataa aggcgactgc accattatca gggactgtgt catgacaggc
     3061 atttccggag gaaaaagaac cataaaaaac aggaaccagg taaaagcagc agattttatt
     3121 aatattttcc tttaaaataa aaactacagg acatgaagca tttgtaccgg attatggtca
     3181 tattcatttt tattgccagc gccagccccg gactgtgggc gcagcagcag gaaatgtcat
     3241 tcggaaagtc cgtgcccctg aaaaatgtaa cacccggaac atctttaaaa agtagtgcac
     3301 tgaagtggat caatgtcaat acggcagaat acacatggtc agtaaaaaaa aatatcctgg
     3361 tttgtaccgg acggccgatt ggcgtgatga gatccgaaaa acaatatgag aatttcatac
     3421 ttcatatcga gtggaagcat atggaaccgg gcggtaattc gggtgttttc gtctggtcag
     3481 aggcaatacc ggatgaaaaa tcccacttac caaacggtgt ggaagttcag atgcttgaac
     3541 ttgaatgggt taaactcaat actaaggacg gaattgaacc accggtcgcc tatgttcacg
     3601 gtgaactgtt cggcgtgggc ggcgtaaaaa caactcccga caatcccagg ggtacccgga
     3661 gcaaatcagt tgaaaaccgg tgcaggggaa gaggtgaatg gaattattat gatgtcgtat
     3721 gtgtggacgg cgtcattaag ctttcggtta acggcaagtt tgtcaatggt ataagtcatt
     3781 ccacccgaaa aaccggatat ctctgcctgg aatcagaagg aggggaaatt catttccgca
     3841 atatcagaat aaccgaactt ccaccaggtg ttatttctga ggaataagcc ccggccatca
     3901 gtttcagtcc cttgccaccc catattcttc ccggcatagc ttcatcagca ggccgctgac
     3961 agaccactcg ccctaaggga ttttccgttc aggctccagg aagagattcc ggcggtttcc
     4021 tgcaatcata attccgattt ctgcaatcat atttccacta atcctgccat ccgttaaccc
     4081 aaacctgcca tagggtgatt ataccttttc tgctaaccgc gttatatata atttcattct
     4141 ctgaaaagat atacggatcc ggaaataaat aaataaaaac aattaataaa actatttact
     4201 aacaaccaaa ttaaactatt aagccatgaa aagaatgatt ttatttgcag tgcttgtttt
     4261 ggcagccggc acattttcgt tcggaacgaa gctgatcggc gaagggaaaa cttacacatc
     4321 ccttggcaat tacagagtgg aggcaatcga cagtcctgta cctctgaagg gcgcagattg
     4381 ccaggcttat gtcattaagt atgagaacac cccgatggag gtaacagtta ttgtctgcag
     4441 ggatcagaag gatcagaaat atcttgtgct ctcagacagg ctttctgttc agtatgtaaa
     4501 caacgctcag tatttcggag ttgaaatggt tgacaaatcc tttgaaaagg aaggctataa
     4561 aacagacaat tcgggactga acaaaattgc gtatttccgt cagaaggtca tcggacccgg
     4621 acagatgcgg gaaaaggatg caacaaggac cattgccgca tttttcccct tccttctgag
     4681 gacggatgaa aatatgactg ctgaaaaaca ataggcactt ttccgggcgc tctgccgggt
     4741 tttgggtttt gaatggaggg accggacagg tccctttttt atttcagccg gcggtatacc
     4801 gcccccgggg gctgtttcag acgaatcttt ccgacaaaaa gtattacttc atgcagcaaa
     4861 caataataaa cactgtcttt aaaataaaat catgaaaagt ctgaagagtt ttacacttta
     4921 aataattaag ccatgaaaag aatgattttg tttgcagtgc ttgttttaac agcagggtct
     4981 tttctcttcg ccaccaaagt ggtggcagaa gggaaaaccc actccacaat gggtgactac
     5041 aaggtgatgg caattgacaa tcctg
//
LOCUS       DAOSPX010000242         5062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15028_length_5062_cov_4.282205, whole genome shotgun sequence.
ACCESSION   DAOSPX010000242 DAOSPX010000000
VERSION     DAOSPX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15028_length_5062_cov_4.282205"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="PLZ75_10755"
     CDS             complement(<1..1582)
                     /locus_tag="PLZ75_10755"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOS72911.1"
                     /translation="MRIRFMLLVPVALAILTGCSGSQNSGGISGNDRGISLKLKKQPV
                     LAGKKRNELLIIDIPPAGSLEQQLPVSRVDIKFEKSSALENLSAIQVYYYEGSDSSSE
                     TSGPAGMPSADEGGISGPSAPALFGSSEKVRNNNRIKGNIPAGDRALKIAVSFTSSGK
                     PDLTAKILVRNIRISFSGGKPVDLQLSPENKILRFGIILREAGQDNCDTYRIPGLITT
                     SSGTLIAVYDNRYNRSKDLQEDIDIGMSRSTDGGESWEPMKVIMDMGGYGGRPERLNG
                     IGDPSILYDHVTNTIWVAALWMSGGSYDEMLWFASGPGMKPEETGQFILVKSTDDGIS
                     WSDPVNITGQIKDPDWQLLLQGPGRGTTMTDGTLVFPAQFKADLGIPALDGGQYTCHS
                     TIVYSKDSGRSWHIGKGAKPNTTEAQVIELGDGSLMLNMRDDLNRRDKSGSNGRAVSV
                     TSDLGQTWTKHPSSNSLLPEPNCMASLIAADMDIGGTIRKVLFFSNPDNRNTRTNMTI
                     KASLDEGISWPEEYRLEIY"
     gene            1748..4102
                     /locus_tag="PLZ75_10760"
     CDS             1748..4102
                     /locus_tag="PLZ75_10760"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOS72912.1"
                     /translation="MKKTLILTGITVFFILVSFSCRTEKTAAVEEDLTKYVDPQIGTA
                     HCRWFHVAPGASPFGMAKPGPSTDGHLGNASGWEAVGYDFRHNSIEGFPNFHEFQIGG
                     VVFMPTNGELRTIPGALDFSDRGYRSKFNKEDEILNPGYYSVILKDYNIRAELTSTDR
                     VSFHRYTFPANDKSHILFDIGNKQGESGEVTAASVVLAEDGHVEGFVETYPEYVKKYQ
                     TGASVRMYFYAILDKKPDEYGIFHGTRTEAGKRTATGRGAGLYLTFSTRDNESINIKA
                     GLSYTSVENARLNLEAEAGNLGFDEARKAAHENWNAWLGRIHVEGNNHEDKVKFYTGL
                     YHALLGRGLASDINGAYPRHDGTNGMIPAGSDNKPEFRFYNTDAIWGGQWNLIQLWAL
                     AYPEYTTDFVKTHLRVYEDAGWLGDGLACSKYVSGVGTNQVPLAIVSAYMCGIRDFDL
                     QKGYEASLKNETVSKNRVFGAGKMDVGEFVKYGYVPYKSTGKGPDELWKFSCSHTLEY
                     SFTSYAVAQFAKALGKNDDYEMLMELSKGWEKIYNPEKKMMWPKFADGSFNGNFDATV
                     PHDGFQEGNAMQYTFYVPHDPRGIAAKIGQEEFNIRLDSTFLISQKDIFGGGTEIHAF
                     AGITKPYNHGNQPCLHTSWLFNYTGEPSKTQKWVRAILNDFYGTEGIHGYGYGQDEDQ
                     GQLGAWYVMSAMGLFDVKGLTEPEPLFSIGSPLFDRIIINLNGNYYSGEQFVIETENN
                     SPENIYIQSVSVNGNNLNSAFIPFKDVVKGGVMLIRMGNEPGDH"
     gene            4127..>5062
                     /locus_tag="PLZ75_10765"
     CDS             4127..>5062
                     /locus_tag="PLZ75_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72913.1"
                     /translation="MQSSRRKFLRKSLASAAGLAAINAFPEGLPFGNLHTTSYPEEII
                     LRPATVRTPGESIRFSVIGLNHDHIYGMSDALIRGGGKLVSVYAPEPELLKTFTKRFP
                     GVRIAGSEKEILEDTSVQLVASASIPVDRAPLGIRVMKSGKDFMADKPGIVTPRQLEE
                     VKKVQKITGRIYSIMYSERLGNPASVQAGNLIEAGAIGKVVQTIGLGPHRMRPASRPD
                     WFFDPEKAGGILCDIGSHQCDQFLFYTGSKQAEVSFAQTGNFANGKYPAFQDFGDMSV
                     RSPHASGYIRIDWFTPDGLNTWGDGRTFILGTEGYI"
BASE COUNT         1396 a         1206 c         1225 g         1235 t
ORIGIN      
        1 cataaatctc gagacggtat tcttccggcc agcttattcc ctcatccagg cttgccttga
       61 tcgtcatatt cgtccttgta tttctgttgt ccggattgga aaagaacaaa accttccgga
      121 ttgtaccacc tatgtccata tcggcagcta tcaggctggc catgcagttt ggttcaggaa
      181 gaagggaatt tgacgacgga tgcttagtcc aggtctgccc gaggtcagat gttaccgaca
      241 cagcccttcc gtttgatccg cttttgtccc tgcggttcag atcatccctc atattcagca
      301 taagactgcc atcacccagc tcaatcacct gtgcttcggt ggtgttgggc tttgctcctt
      361 ttccgatatg ccagctgcgg ccgctgtcct tactgtatac aatggtggaa tggcaggtat
      421 actgcccgcc gtcaagggca ggaattccca gatctgcctt gaactgagca ggaaagacaa
      481 gagtaccgtc tgtcatggtt gttccccgtc cgggaccctg aagcagcagc tgccagtctg
      541 gatcctttat ctggcccgtt atgtttacag ggtctgacca gcttattccg tcatccgtgc
      601 ttttcaccag tatgaactgg cctgtctcct ccggcttcat tcctggtcct gatgcaaacc
      661 acagcatctc atcataagaa cctccgctca tccagagtgc tgcaacccag atggtattgg
      721 tcacatgatc ataaagaata ctgggatccc ctataccgtt cagcctttcc ggccttccac
      781 cgtatccgcc catatccatt atgaccttca tgggttccca gctctcccct ccgtcagtgc
      841 ttctgctcat tccaatgtca atgtcttcct gcagatcctt gcttctgtta tacctgttgt
      901 cataaaccgc aatcagtgtc ccggaactgg tcgtgataag cccgggaata cggtaggtat
      961 cacaattatc ctgtccggcc tccctgagaa taataccgaa acgaaggatt ttgttttcag
     1021 gggataactg caaatcaaca ggtttcccgc cggaaaagga tatccggata ttcctgacaa
     1081 gaattttcgc agtcagatcg ggttttccgg aagaagtgaa ggaaacagct attttcaggg
     1141 cccggtcacc tgccggaata ttacccttta tcctgttgtt gttacggacc ttttcactgc
     1201 tcccgaagag tgcaggagca gaggggccgg atattccgcc ttcgtcagca gacggcatgc
     1261 cagccgggcc ggatgtttca gaggaggaat cagatccttc ataataataa acctgtattg
     1321 cggaaagatt ttcaagagct gatgattttt caaattttat atccaccctg ctgaccggaa
     1381 gctgctgctc caatgagccg gccggtggaa tgtcaattat taaaagctca ttcctcttct
     1441 ttccggcgag aacgggctgt tttttcagtt tcaggctgat tcccctgtca ttcccggaaa
     1501 tcccgcctga attttgtgat cccgaacagc cggttaaaat ggcaagagcc acgggaacca
     1561 acaacataaa ccttattctc atatcattac tatgattaac gaggtaaaat aaactcgtca
     1621 ataaatatac aaattttatg tatttttaga aattccacaa tctcctgtcc ggcaggggaa
     1681 cagattttca aaaaacaaaa acactatgga agcaaagaaa aatccagaat ccataaacca
     1741 caattttatg aaaaagactc tgatcttaac aggtattaca gtttttttta ttcttgtttc
     1801 attttcgtgc agaacggaaa aaacagcagc cgtggaagaa gatctcacga aatatgtcga
     1861 tccgcagatc ggaaccgctc attgcagatg gtttcatgta gccccgggag caagtccttt
     1921 cggaatggct aaacccggtc cttctactga cggacatctc ggaaatgcca gcggatggga
     1981 agccgtgggc tatgatttca ggcacaattc aattgaaggc tttccgaatt tccatgaatt
     2041 ccagatcgga ggtgttgttt tcatgccaac caacggtgaa ctcagaacaa ttccgggtgc
     2101 ccttgacttt tccgacaggg gataccgctc aaaattcaat aaagaggacg agatactcaa
     2161 ccccggctat tattcagtaa ttcttaagga ttacaatatc agggcagagc tgacttcaac
     2221 cgacagggtc agctttcacc gctacacttt tccggcaaat gataagtcgc atatcctttt
     2281 tgacatagga aacaagcagg gcgagagcgg tgaagtcacg gctgcatccg ttgtccttgc
     2341 cgaagacggg catgttgagg gctttgtgga aacatatccc gaatatgtaa agaaatacca
     2401 gaccggcgcc agcgtcagaa tgtactttta tgccatactt gataaaaagc cggatgagta
     2461 cggaatattt cacggaacaa ggacggaggc cggaaagagg acggcaacag gcaggggagc
     2521 aggtctctat cttacctttt ccacccggga taatgaaagc atcaatatca aggcaggcct
     2581 gtcatacacc tcggttgaaa atgcacgcct caaccttgaa gcagaagccg gaaaccttgg
     2641 ttttgatgag gccagaaaag ccgcacatga aaactggaat gcatggctgg gaagaattca
     2701 tgttgagggc aacaatcatg aagacaaggt aaaattttac accggacttt atcatgccct
     2761 gctcgggcgt ggtctggcca gcgatatcaa cggagcctat cccaggcacg acggaaccaa
     2821 cggcatgatt cccgcgggaa gcgacaataa gccggaattc aggttttaca acaccgatgc
     2881 aatatgggga gggcagtgga acctgattca gctgtgggcc ctggcctatc ctgaatacac
     2941 tactgatttt gtaaagacac atctccgggt ttatgaagat gcaggatggc ttggtgatgg
     3001 cctggcctgc agcaaatatg tttcaggggt gggaaccaac caggtgcccc tggccattgt
     3061 aagcgcatat atgtgcggca tcagggattt tgacctgcag aaaggctatg aagcctccct
     3121 caaaaatgaa acggtttcaa aaaacagggt gtttggtgca gggaaaatgg atgtgggtga
     3181 atttgtgaaa tatggctatg tcccgtacaa atcaaccggg aaaggtccgg atgaattgtg
     3241 gaaattctcc tgttcacaca ccctggaata ttcatttact tcatatgctg ttgcacaatt
     3301 tgccaaagcc ctggggaaaa acgacgatta tgaaatgctg atggaactgt caaaaggatg
     3361 ggagaaaata tataatcctg aaaagaaaat gatgtggccg aaattcgcag atggcagttt
     3421 taacgggaat tttgatgcca cggtgccgca tgacgggttc caggaaggaa atgccatgca
     3481 atatacattc tatgtccccc acgatccccg gggaattgca gccaaaatag gacaggaaga
     3541 attcaacatc aggctcgaca gcaccttcct tatctctcag aaggatatat tcggaggggg
     3601 aacggagatc catgcatttg caggaataac aaagccctac aaccacggaa atcagccctg
     3661 tctgcatact tcatggctgt tcaactatac cggtgagcct tcaaaaaccc agaaatgggt
     3721 aagggcgata ctgaatgact tttacggcac ggaaggcatt catggctatg gctacgggca
     3781 ggatgaggac cagggacagc ttggcgcctg gtatgtaatg tcggccatgg gcttgtttga
     3841 cgtaaagggg ctgacggaac ccgagcccct tttcagcatc ggaagtccgc tttttgacag
     3901 gataataatc aatctgaacg gcaattatta ttccggagaa caatttgtta ttgaaacaga
     3961 aaataacagc ccggaaaata tttatattca atcagtatct gtaaacggaa acaatctgaa
     4021 ctcagccttc atccccttta aggacgttgt aaaaggagga gtgatgctga tcaggatggg
     4081 caatgaaccc ggagatcatt aacaaaaatc ttaaatccag actaatatgc aatccagcag
     4141 acgtaaattc ctgagaaaat cccttgcctc tgctgccgga ctggcagcaa taaatgcttt
     4201 tcccgaaggc ctgcctttcg gaaatctgca cacaaccagt tatcctgagg aaataattct
     4261 cagaccggca actgtaagga cacccggaga atcaatcaga ttttcggtca taggcctgaa
     4321 ccatgaccat atttacggga tgtctgatgc actcatcagg ggagggggaa aactggtttc
     4381 agtttatgcc ccggaaccgg aattgctaaa aacatttaca aaaagatttc ccggtgtcag
     4441 aattgcagga agcgagaagg aaatacttga ggatacatca gttcagctgg ttgccagcgc
     4501 atccatccct gtcgaccggg ccccgctggg catacgtgtg atgaaatcgg gcaaggattt
     4561 catggccgac aaaccgggta ttgtaacacc acggcagctt gaggaggtga aaaaggtaca
     4621 aaagatcacc gggcgtatct attccatcat gtacagcgaa cgcctgggaa atcccgcctc
     4681 ggtacaggcg ggcaacctga tagaggccgg agccatcggg aaagtggttc agaccatagg
     4741 tctcggaccg cacagaatga ggcccgcttc ccgtccggac tggttttttg atccggaaaa
     4801 ggcaggcggt attctatgtg acatcggatc ccaccagtgc gaccagttcc ttttctatac
     4861 cggttcaaaa caggcggaag tcagttttgc acagaccggg aattttgcca acggaaaata
     4921 tcccgcattt caggattttg gcgacatgag cgtgagaagt ccgcatgcaa gcggatatat
     4981 ccggatagac tggttcacac cggacggcct gaacacatgg ggagacggca gaacctttat
     5041 tctgggaacg gaaggatata ta
//
LOCUS       DAOSPX010000243         5057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15058_length_5057_cov_4.141543, whole genome shotgun sequence.
ACCESSION   DAOSPX010000243 DAOSPX010000000
VERSION     DAOSPX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15058_length_5057_cov_4.141543"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2596
                     /gene="ppdK"
                     /locus_tag="PLZ75_10770"
     CDS             <1..2596
                     /gene="ppdK"
                     /locus_tag="PLZ75_10770"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOS72914.1"
                     /translation="FTITTEACTEYYENNHTLHPELMPEVWDAMKSTEKSMGMKYGDP
                     ENALLVSCRSGARRSMPGMMDTVLNIGLCSATIPGFLKKTNNPRFVWDSYRRLIMMYA
                     DVVMEKAEGIDPVDGQGIRKTLEEKFEEFKKKKGYESDTDIQADDMKYIADEFKKTVK
                     EVLGKPFPDDAREQLEGSIKAVFKSWNGKKAVSYRRIEGIPDEWGTAVNVQAMVYGNM
                     GDTSATGVAFTRNPATGENLFYGEWLVNAQGEDVVAGIRTPSPLNNETKNEQNMHLKS
                     MEEAMPEAYRELRNIRDILEKSFRDMQDVEFTIQENVLYMLQSRTGKRTGTAALNMAM
                     DMLHEGLIDEQTAVMRVDPAQLDELLHPICDPEAEKKLTPLVKGLPAGPGAAVGRVVF
                     SADDAVAWSRRGEKVILLREETNPEDVEGMRVAEGILTARGGMTSHAALVARGWGKCC
                     IVGAGALKVDVNAKTAKINGTDTVISEGDVLTLNGTKGNVYSGSVNLIDASENPRFRE
                     FMTMVDKYRTMGVRTNAETPEDARVALDFGAEGIGLFRTEHMFYGKDSEKPLFLLRKM
                     ILSASESERRQALDELLGYTKKDMKETLEAMDGKPVTFRLLDPPLHEFVPHSSDKQEE
                     LAKALGISLEEIIKRGEALSESNPMMGHRGVRLSITYPEVTEMQVRAMFEASMELIRE
                     GRKPLPELMVPVTCDVSELDVTKVTVDKIHDEVCRKFGVDKINYSYGTMIEIPRAAML
                     SDRMARSAEFFSFGTNDLSQMVFGFSRDDIGGFLHDYLDKKMLQADPFQTIDVDGVGQ
                     LITMSVEKGRSTRPNLKIGICGEQGGDPASVEFCYKNGLTYVSCSPYRVPIARLAAAQ
                     AAIKFGKR"
     gene            3033..4886
                     /locus_tag="PLZ75_10775"
     CDS             3033..4886
                     /locus_tag="PLZ75_10775"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOS72915.1"
                     /translation="MKRFLQVIQEGIIKNTFMTKNEKLNGWVKEWAELCQPDSIHWCD
                     GSEEENQMLMDMMVKNGNCIKLNDDKRPQSYYFQSDPSDVARVENRTFICSVKEEDAG
                     PSNNWSDPAVMKDTLRPLYRGCMKGRTMYIIPFSMGPLGSEISHIGIEITDSPYVVAN
                     MRIMTRMGKAVLDILGEDGSFVPCVHSVGAPLQPGEKDRKWPCAPDIDNKYITHFPET
                     NEIWSFGSGYGGNALLGKKCFALRIASNMARREGWLAEHMLIMGITSPRGVKKYFAAA
                     FPSACGKTNLAMLIPTIPGWKVETVGDDIAWMKFGEDGRLYAINPEYGFFGVAPNTSM
                     KTNPNAMLSCRANTIFTNTALTPDGDIWWEGIGYDIPEGSITWTDEKYDPASGKPASH
                     PNARFTAPAGQCPVIDSDWENPRGVPVSAILFGGRRPDTLPLVYQSFDWNHGVFVGSI
                     AGSEVTAAAIGLKAGVRRDPFAMLPFCGYHMADYFAHWIKIGETAPDKNKLPRIFNVN
                     WFRKDAGGNYLWPGYGDNIRALKWIFERLEGADNAVETAVGYVPKPESIDISGLEGIE
                     KNLPELLKVDRNEWLAETELVAGHFESFGRKLPEELKKQFSDLRSRLDRIQ"
     gene            4914..>5057
                     /locus_tag="PLZ75_10780"
     CDS             4914..>5057
                     /locus_tag="PLZ75_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOS72916.1"
                     /translation="MSVIKILTAVVTAYLLGSLPSAVWAGRIFHGIDVREHGSGNAGA
                     TNVI"
BASE COUNT         1515 a         1132 c         1290 g         1120 t
ORIGIN      
        1 cttcaccata accacagaag cctgtacgga gtattatgaa aacaatcata ctcttcatcc
       61 tgaactgatg cctgaagtat gggacgcaat gaaaagcacc gaaaagagca tgggaatgaa
      121 atatggcgat cctgaaaatg cattactggt atcatgccgt tcaggagctc gcagatccat
      181 gcccggaatg atggataccg tcctcaatat cggtctctgt tctgccacaa tccccggttt
      241 tctcaagaaa acgaacaatc cccgctttgt atgggattcc taccgcaggc tgataatgat
      301 gtatgccgat gtggttatgg aaaaggccga aggaatagac ccggttgacg gacagggcat
      361 aagaaagaca cttgaagaaa aattcgagga gttcaaaaaa aagaaaggct atgaatcaga
      421 tactgatatt caggctgatg atatgaaata tattgccgat gaattcaaga aaaccgttaa
      481 ggaagttctc ggcaaaccat ttccggacga tgccagggag cagcttgaag gaagtattaa
      541 agctgtcttt aaaagctgga acggaaagaa agccgtatcc taccgccgca tagaaggtat
      601 tcctgatgaa tggggaacgg ctgtaaacgt acaggcaatg gtgtatggca atatgggtga
      661 cacatctgca accggtgttg cattcacgcg taaccctgca accggtgaaa acctctttta
      721 cggcgaatgg ctggtgaatg cccagggtga ggatgtcgtt gcaggtatcc gtacccccag
      781 ccccctgaac aacgaaacaa agaacgagca gaacatgcat ctgaaaagca tggaggaagc
      841 catgcccgaa gcatacaggg aactgcgcaa catacgcgac atacttgaaa aatcattccg
      901 cgacatgcag gacgtggagt ttacaatcca ggagaatgtc ctttacatgc ttcagagccg
      961 tacaggtaaa agaacaggaa cagcagccct gaatatggca atggacatgc ttcatgaagg
     1021 cctgatagat gaacagaccg ctgtaatgag ggttgatccg gcacagcttg atgaactgct
     1081 ccatccaatc tgtgatcctg aagcagaaaa gaaactgact ccccttgtga aaggtcttcc
     1141 cgccggacca ggtgctgcag tgggcagagt ggtattttca gcagatgatg cagtggcatg
     1201 gtcgcgcagg ggtgagaaag ttatcctcct ccgcgaggaa accaatccgg aagatgttga
     1261 aggtatgaga gtagccgagg gaatacttac tgcacgcggc ggaatgacct cacatgccgc
     1321 acttgtggca cgcggctggg gaaaatgctg tatagtggga gcaggtgccc ttaaagttga
     1381 tgtgaatgcc aaaacagcga agataaacgg aacggatacg gttatcagtg aaggtgatgt
     1441 cctgactctt aacggaacaa aagggaacgt atacagcggt tccgtaaacc tcatcgatgc
     1501 atcggaaaat cccagattca gggaattcat gacaatggtt gacaaatacc gtacaatggg
     1561 tgtccgtacc aatgcggaga cacctgaaga tgcaagggtg gcactggatt ttggagctga
     1621 aggaatagga ttgttccgga ctgaacacat gttctacggt aaggattctg aaaagccatt
     1681 gttcctgctg aggaaaatga tactcagtgc aagtgaaagc gaacgccgcc aggcactgga
     1741 tgaactcctc ggatatacca agaaggatat gaaggaaacc ctggaagcta tggacggaaa
     1801 gcctgtcaca ttcagacttc ttgatccccc gcttcatgaa tttgttcccc acagttctga
     1861 caagcaggaa gaactggcaa aagctcttgg aatcagtctg gaagaaatta tcaagagagg
     1921 agaagcactc agtgaatcaa atccgatgat gggacaccgc ggcgtacgcc tttcaataac
     1981 atacccggaa gttaccgaaa tgcaggtaag ggcaatgttt gaagcttcaa tggaacttat
     2041 cagggaaggc aggaagcccc ttccggaact gatggtaccg gttacctgtg atgtgtcaga
     2101 actcgatgtg acaaaagtaa cggtggataa gatccatgat gaggtatgca ggaaattcgg
     2161 cgttgataaa atcaattaca gctacggtac aatgattgaa ataccgaggg cagcgatgct
     2221 ttccgaccgg atggccagat cagccgagtt tttctccttc ggaaccaacg acctttcaca
     2281 gatggtattc ggattcagcc gtgatgacat aggaggattc ctccatgatt atctcgacaa
     2341 gaaaatgctt caggctgatc ccttccagac catcgatgtt gacggtgtgg gacagctcat
     2401 cacaatgtca gtcgagaaag gccgctcaac caggccaaac ctgaaaatag gaatctgcgg
     2461 ggaacaggga ggtgatccgg cttcagtaga attctgctac aaaaacggac ttacatatgt
     2521 aagctgctca ccctaccgcg ttccgatagc cagactggcc gcggcacagg ctgcaattaa
     2581 attcggaaaa agataaaata agcaattgag agaatgaagg gcgttgaaaa ccaacgccct
     2641 tttcttttaa aaagctcctg ataagcgtat acagttaaag aggccgggga atgaagccgc
     2701 gcaacgcggg aagacccggt taacggaaga atatccggca gaaaaaaagc agcaggcgga
     2761 caaaagctgt cagtcccgta aaagcaccgg gaacggaaga atatccggca ggaaaaaaca
     2821 ggaaccaaat cccggactgc agatttcttc ccgccattat tatcagtaaa tcagaatata
     2881 acattttcat taagatataa gtcatatttt ctgaatatat tgtatcaata tacggatatt
     2941 aatttataaa atgtatattc caaattattt tattaacaaa atgatatttt ccatatcatg
     3001 atttgcatca gaaacggaat ctatttatat atttgaagcg gtttttacaa gttattcagg
     3061 aaggaataat aaaaaataca tttatgacaa aaaatgagaa attaaacgga tgggtaaagg
     3121 aatgggctga gctttgccag cctgacagta tccactggtg cgacggctcc gaagaggaga
     3181 atcagatgct gatggatatg atggtaaaaa acggaaactg cattaaactc aatgatgata
     3241 aacgtcccca gagctattat tttcagagtg accccagtga tgtggcccgt gtggaaaacc
     3301 ggaccttcat atgttccgtg aaggaggaag atgccggtcc ttccaacaac tggagcgacc
     3361 cggccgtaat gaaggatact ctccgcccgc tgtaccgggg ctgcatgaaa ggccgtacaa
     3421 tgtatatcat acccttcagc atgggtccgc tcggatcgga gatctcacat atcggtattg
     3481 agataaccga ttcgccgtat gttgttgcca atatgaggat aatgacccgt atgggaaagg
     3541 cggtccttga catactggga gaagacggct catttgttcc ctgtgtgcat tccgtcgggg
     3601 caccccttca gccgggtgaa aaagacagga aatggccctg tgcaccggat atagacaata
     3661 aatacattac acactttccc gaaaccaatg agatatggtc attcggcagc ggttacggag
     3721 gcaatgcact cctcggcaag aaatgctttg ccctcaggat tgcttccaac atggcccgca
     3781 gggaaggatg gcttgcggaa catatgctta ttatgggcat taccagtccg cggggcgtga
     3841 agaaatattt tgccgccgcc ttccccagtg catgcggaaa gaccaacctt gccatgctta
     3901 ttcccaccat tccgggatgg aaagtcgaaa cagtgggcga tgacatagcc tggatgaaat
     3961 tcggggaaga cggacgcctg tatgccatca atcccgaata cgggtttttc ggcgtggctc
     4021 ccaatacttc catgaagacc aatcccaatg ccatgctttc atgcagggca aataccatat
     4081 ttaccaatac agcccttacg ccagacggag atatctggtg ggaaggaata ggctatgaca
     4141 tacctgaggg ttcaataaca tggacagatg aaaaatacga ccctgcatcc ggcaagcctg
     4201 catcccatcc caatgcccgt ttcaccgccc cggcaggcca gtgtcccgta atagacagcg
     4261 actgggaaaa tccccgcgga gttccggtat cggctattct cttcggtggc cgcagacccg
     4321 acacactacc tcttgtatat cagtcattcg actggaacca tggtgttttc gtgggttcca
     4381 tagcaggttc agaagttact gccgccgcca ttggtctcaa ggcaggagtc cgccgtgatc
     4441 cctttgcaat gcttcccttc tgcggatatc acatggcgga ttattttgca cactggataa
     4501 aaatcgggga aacggcaccc gataaaaata aacttccccg catattcaat gtaaactggt
     4561 tcagaaagga tgccggaggc aactacctgt ggcccggtta cggagacaac ataagggctc
     4621 tcaaatggat atttgaacgc cttgaaggag ccgacaatgc ggtggaaact gctgttggat
     4681 atgtaccaaa gcccgaaagc atagatattt ccggccttga gggaatagaa aagaatctgc
     4741 cggaactgct gaaagtggac aggaatgagt ggctggctga aactgaactg gtggcaggcc
     4801 atttcgaaag cttcggccgg aaactgccgg aagagctgaa gaagcagttc agtgatctca
     4861 ggtcacgcct tgacaggatt caataacccg gccggtattc aataaatttt cgaatgtctg
     4921 ttatcaagat actgacagcc gtggtcaccg cctaccttct cggatccctt ccgtcggcag
     4981 tatgggcagg aaggattttt cacggcattg acgtgaggga gcacggaagc ggaaatgccg
     5041 gagcaacaaa tgttata
//
LOCUS       DAOSPX010000244         5055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15061_length_5055_cov_4.424000, whole genome shotgun sequence.
ACCESSION   DAOSPX010000244 DAOSPX010000000
VERSION     DAOSPX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15061_length_5055_cov_4.424000"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLZ75_10785"
     CDS             <1..801
                     /locus_tag="PLZ75_10785"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOS72917.1"
                     /translation="KIELVRPYVAAVNFTDSSSASPRMSSLACSGVAAGLDAEAVLQI
                     TARDTTRTGLQAMATGVARFGVKNILCLTGDSPGVGPSPAASLNFTDLDSVQMLWILR
                     RMRDEGIYLDGRKTKYPPEYFLGAASSPFASEPVLQAIKDQKKVNAGAQFLQTNIVFD
                     PERLDLWLEELDKRSVLDKVFILVGVATMKSLKLARYLHYRVPGVFLPDRILARMEKA
                     GDGEAEEGFQIALETITSLKNKKGINGLHIMTLGWEEIVGRIVKESGL"
     gene            798..1484
                     /locus_tag="PLZ75_10790"
     CDS             798..1484
                     /locus_tag="PLZ75_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOS72918.1"
                     /translation="MMYETFSFDFRQLGITVTQLQKDMGYRKEAPDASVDLIISEVLE
                     ECETLGSIRAEYRIFENIEFDPLSRGLKIENIFFGINEIVFNHLAGSELIAVFLSTAG
                     EETAIRGREAANAGDPLKGFAYDVIGNEIVEGAAELMNKELDKRFVREGMNLTNRFTP
                     GYCGWNVSDQHKLFTLMPDNFCDISLTESALMKPVKSISGFIGIGREAKRVHSVCRFC
                     NMKDCFYRRP"
     gene            complement(1635..2540)
                     /locus_tag="PLZ75_10795"
     CDS             complement(1635..2540)
                     /locus_tag="PLZ75_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS72919.1"
                     /translation="MKNFLSRGLMFTSGLILSFSLSAAKITAEKTGSKINVTINGRFF
                     TSYVFSEDEKYPFFYPLNGPVSGGSVTSMRNAVYPHHTSLFFGCDMVNGGNYWQEGLE
                     RGRIISVNARIEKQGGDSVVISDECIWSRPGALSPVKDSRKYIISSPSETIYQIDVEI
                     SMEMLMDVVIRKTNHSLFSARMAADLAVTSGGTMINAEGAKGEKDTFGKNSAWMDFYG
                     KRGNAVEGLAILQHPSNPWYPSPWFTRDYGFMSPTPMYWPPNDTDTQLKKGTALVLRY
                     RVLVHSGTHTDADIAGQFDKYKVLR"
     gene            complement(2551..3924)
                     /locus_tag="PLZ75_10800"
     CDS             complement(2551..3924)
                     /locus_tag="PLZ75_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS72920.1"
                     /translation="MKRRKFISDTVAAAAGTIVMPVIIPSSVMGKNPPSERINIGWIG
                     CGRQGRGNIMATMRFDSARVVAVSDVDSNRMALGKKMIEDFYSRKTGSSNAITVRSYD
                     DYKDMLLNKSIDAVMITTPDHWHAQPAIEAALAGKDIYLEKPTSLTVAEGRLLSDVVR
                     KRKIILQVGTQQRSSPQWRIAAELVRNGRIGKLHTVKIGLPGDPAGPEAPEMPVPKNL
                     NYDMWLGSTPLVYYTEIRVHPQKDFDRPGWLRCEQFGAGMITGWGQHHFDSAAWGMDT
                     ELTGPVSVQAVAQFPKSGLWDVHGDFMSITEYRNGITQITSGSYPNGVRYEGSEGWIF
                     VTRGAYTATASDPVTVDKNAKALDASDPKILKSVIGPNEIHLYESDSQQGSWLDCIKS
                     RKEPISPVEPGHRACTICLIVHIAMKLGRKLNWDPDNERFINDDEANSMLSRPQRAPY
                     GTNYIKM"
     gene            complement(3993..>5055)
                     /locus_tag="PLZ75_10805"
     CDS             complement(3993..>5055)
                     /locus_tag="PLZ75_10805"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS72921.1"
                     /translation="EPNKALPENKERIFLFVSKEALTEDGASDKIIIMRNALPYWGGA
                     GKIKTPSGLPGTTDQPLGNLVGGNPVEIDLVTPYGRGVAFSRPSPWAQFELWDDPNDQ
                     RHKYPNWIRMQDLVYNHPGLKAAGDSYYGKHLQLRDAGGGILCTDTIRSWFDWPQYKM
                     YIPDPTNRMPQGGNGDWYCYRLAETYLLRSEAYFWKDDLVNAAADLNAVRTRAGCAPY
                     EPGQINIGTILDERARELYYEEMRNVEITRVAFILARTGKTCYNGKTYSMTNFSSDNF
                     WYDRIMEKNRFYSENVVAPFYTYRVAPWIVLWPIPSDAINANTMGHINQNKGYPGAEN
                     NITPRKWIDGPGEGEIVEQ"
BASE COUNT         1231 a         1160 c         1208 g         1456 t
ORIGIN      
        1 aagattgaac ttgtcagacc ttatgtggcg gcggtcaatt tcacggattc atcctcggca
       61 agtccgagaa tgtcaagtct ggcctgttcc ggagttgcgg caggcctgga tgcggaagca
      121 gttcttcaga taactgcccg ggataccaca agaaccggtc tccaggctat ggccaccggt
      181 gttgcccgtt ttggcgtgaa aaacatactc tgccttacag gtgacagtcc gggggttggt
      241 ccatcgccgg cagccagcct gaattttaca gaccttgatt cggtgcagat gctctggata
      301 ttgaggagaa tgagagatga gggaatctat ctcgacggaa ggaaaacaaa atatcccccg
      361 gagtattttc tgggtgcagc ttcttctccc tttgcttcag aaccagttct tcaggccata
      421 aaagatcaga agaaagttaa tgccggagca cagtttcttc agacaaatat tgtttttgac
      481 ccggaaagac ttgatctctg gcttgaggag ctcgacaaaa ggagtgttct tgacaaggtg
      541 ttcatccttg tcggcgtggc tacgatgaag agcctgaagc ttgcgagata tcttcattac
      601 agggttccgg gggtatttct tcctgacagg atccttgccc ggatggaaaa ggcaggagac
      661 ggggaagcag aggaaggctt tcagattgca ctggaaacca tcacttccct gaaaaataaa
      721 aaagggatta acggattgca tattatgacc ctcggatggg aagagatagt gggaagaata
      781 gtgaaagagt ccggactatg atgtatgaaa ccttcagttt tgacttcaga cagctcggga
      841 taactgttac acagctgcag aaagacatgg gttaccggaa agaagcccct gacgcttctg
      901 ttgacctgat tatatcggaa gttctggagg aatgtgaaac actgggcagt ataagggcgg
      961 agtacaggat ctttgagaat atagaatttg atcctctgtc aaggggactc aaaatagaga
     1021 acatcttttt cggtataaat gaaattgttt ttaatcatct ggccggatcg gaactaatag
     1081 ctgtttttct cagtacagca ggtgaagaaa ccgccatccg gggcagggaa gccgctaatg
     1141 ccggagatcc gctcaagggc tttgcctatg atgtaatagg caatgagatt gttgaaggag
     1201 ctgcagagct gatgaataaa gaactggata aaaggtttgt ccgtgaaggc atgaatctga
     1261 caaaccgttt tacgcccgga tactgcggct ggaatgtgag cgatcagcat aagttattta
     1321 ctcttatgcc ggacaatttc tgtgatatat ccctgacgga atccgccctg atgaaaccgg
     1381 taaaatccat tagcggcttc ataggcatcg gcagggaagc aaaacgtgtt cattcggtct
     1441 gccgtttttg caacatgaaa gactgttttt accgtcgacc gtaataagga ttttacagtg
     1501 tccatagaag tttcacaggg ccggctgagg ttattatgga attatgaaaa agcttcctaa
     1561 taccagacaa agaatttaca tggtccggca gaaacttcac ctgatcagac aaaggattac
     1621 aggatcatcc ggatttatct gagaacctta tatttatcaa actgtccggc tatatccgca
     1681 tctgtatgag ttcctgaatg aaccagcacc ctgtatctca gcacaagcgc ggtgcctttt
     1741 ttcaattgag tatcagtatc gttcgggggc cagtacatag gagtcggcga catgaatcca
     1801 taatctctgg tgaaccacgg cgaggggtac cagggattgg aaggatgctg taaaattgca
     1861 agtccttcca cagcattacc cctttttccg taaaaatcca tccatgcaga atttttaccg
     1921 aaagtatcct tttctccctt tgctccttcg gcatttatca tggtgccgcc gctggtgact
     1981 gccaggtcgg ctgccatcct tgcgctgaaa agtgaatggt ttgtttttct gatcacgaca
     2041 tccatcagca tttccatact gatttccaca tcgatctgat aaattgtttc cgaaggagag
     2101 cttattatgt atttcctgct atcctttacc ggggaaaggg ctcccggccg gctccatatg
     2161 cattcgtcac ttattaccac cgaatcaccg ccctgttttt ctatacgggc atttactgaa
     2221 ataattcttc cgcgttcaag tccttcctgc cagtagttgc ctccgttcac catgtcgcag
     2281 ccgaaaaaga gggatgtgtg atgggggtaa acggcgtttc tcattgatgt taccgaacct
     2341 cccgaaacag gtccgttcag gggataaaag aaaggatatt tctcatcttc agaaaaaaca
     2401 tagctggtaa aaaacctgcc gtttattgtg acatttattt ttgatccggt tttttcggca
     2461 gtaatctttg ctgctgacag ggaaaaggat agtatgaggc ccgatgtgaa catcaggcct
     2521 ctggaaagaa aatttttcat tacaggtatg ttacattttt atataattgg tcccgtatgg
     2581 agccctctgc gggcgtgaaa gcatactgtt tgcctcatca tcattaatga atctctcatt
     2641 atcggggtcc cagttcagtt tcctgcccag tttcattgct atatgtacga taaggcagat
     2701 tgtgcaggcc cggtggccgg gttcaacagg agatattggt tcttttcttg atttgatgca
     2761 gtcgagccag ctaccctgct gtgaatcact ctcgtagaga tgaatttcat ttggtccgat
     2821 taccgatttc agaattttgg gatcacttgc atcaagtgcc ttggcatttt tatccactgt
     2881 taccgggtcg cttgcagttg ctgtatatgc tccgcgggtt acgaagatcc agccttcact
     2941 tccttcatat ctgactccgt tgggatagga accgcttgta atctgtgtga ttccgttcct
     3001 gtattccgtt attgacatga agtcaccgtg gacgtcccat agtcctgatt tgggaaactg
     3061 ggcaacggcc tgaaccgaaa ccggtccggt cagttcggta tccattcccc atgcagcgga
     3121 atcaaagtga tgctgtcccc agcctgtaat cataccggcc ccgaactgct cacatctgag
     3181 ccagcccggc ctgtcgaaat ctttctgcgg atgtacgcgg atttcggtat aatagaccag
     3241 gggggttgat ccgagccaca tatcataatt gagatttttg ggaacaggca tttccggggc
     3301 ttcgggccct gccggatctc ccgggagtcc gatttttacc gtgtggagtt taccgattct
     3361 gccgttgcgt acaagctcgg cggcgattct ccactggggg cttgagcgct gttgtgtccc
     3421 cacctgcagg attattttcc ttttccgtac cacatcgctg agcaatcttc cttcggcaac
     3481 cgtgagtgat gttggttttt cgagatagat atcctttccg gccagggcgg cttcaattgc
     3541 cggctgggcg tgccagtggt ccggggtggt gatcatgacg gcatctatcg atttgtttag
     3601 gagcatatcc ttatagtcat cataggatct gaccgtgatg gcatttgaac ttccggtctt
     3661 acggctgtag aaatcctcaa tcatcttttt gccaagtgcc attctgttcg aatcaacatc
     3721 agaaactgcc accactcttg ccgaatcaaa tctcattgtg gccattatat tgcctcttcc
     3781 ctgccttccg cagccaatcc accctatatt tatcctttcg ctcgggggat tttttcccat
     3841 caccgaggag ggtatgatta caggcatcac aattgtaccc gctgctgcag cgactgtgtc
     3901 agaaatgaat tttcttcttt tcatatattt tttccggttt attaaagggt tattaataca
     3961 tgtaaattta acaattagta cggacagagg tattattgtt caacaatttc tccttctccg
     4021 ggtccgtcaa tccattttct gggagtgata ttgttttctg ctccaggata acccttgttc
     4081 tggttaatat gtcccatggt attggcattt attgcatctg aaggaattgg ccaaagtaca
     4141 atccagggag caaccctgta ggtataaaac ggggctacaa cattttcgga ataaaaccgg
     4201 tttttttcca taatcctgtc ataccagaaa ttatctgaag aaaagtttgt catgctgtat
     4261 gtttttccgt tatagcatgt ttttcctgtc cgggccagaa tgaaagcaac acgggtgatt
     4321 tccacgtttc tcatttcttc gtaatataat tcgcgtgcac gttcgtcaag tatggtaccg
     4381 atatttattt gtccgggttc atacggggcg cagcctgccc ttgtgcgtac ggcattgaga
     4441 tcggctgcag catttacaag atcatccttc cagaaatagg cttcagatct caaaagatat
     4501 gtttcagcca gtctgtagca gtaccagtca ccatttccgc cctgcggcat tctgttcgta
     4561 ggatcaggaa tatacatttt atactgaggc cagtcaaacc atgacctgat ggtgtcggtg
     4621 cacagaatgc ccccgcctgc atcacgaagc tgcagatgct tgccgtaata tgaatctccg
     4681 gcagctttta atcccgggtg attatataca agatcctgca ttcttatcca gtttggatat
     4741 ttatgacgct gatcattcgg atcatcccag agttcaaact gggcccaggg tgatggtctg
     4801 gaaaacgcta ctcctcttcc gtatggtgta accaggtcta tttctaccgg attacctccg
     4861 accaggttgc ccagtggctg gtcagtcgtg cccggcaggc ctgacggtgt tttgatttta
     4921 cccgcacctc cccagtaagg gagggcattg cgcatgatta ttatcttgtc gcttgcaccg
     4981 tcttccgtca atgcttcctt ggaaacaaaa aggaatatac gttccttgtt ttccggaaga
     5041 gctttgtttg gttcc
//
LOCUS       DAOSPX010000245         4979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15356_length_4979_cov_4.187449, whole genome shotgun sequence.
ACCESSION   DAOSPX010000245 DAOSPX010000000
VERSION     DAOSPX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15356_length_4979_cov_4.187449"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLZ75_10810"
     CDS             <1..430
                     /locus_tag="PLZ75_10810"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOS72922.1"
                     /translation="YFFYKGADLIIGSHPHVLQKMVWKKDNPVFRNKAIVYSLGNFVS
                     NQRKPLTDGGSMVRIEVARRDSSVVITDAGYFLTWVYTPVEDGKRQFYILPCSEFENR
                     PDFFHDRNDFLQMQRFINTSRSLLNRHNEGFRELYGKEAE"
     gene            524..1171
                     /locus_tag="PLZ75_10815"
     CDS             524..1171
                     /locus_tag="PLZ75_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOS72923.1"
                     /translation="MNYDYNTQRKRMALPEYGRNVQKMADHIKTIKDRNERNRAARTL
                     IQIMGNLNPHLRDEGDFKHKLWDHLALIADFELDIDSPYPPPERGKFAEKPNEIPYRQ
                     GDIKFLHYGGIIEIMIDAACKLEDGPEKEYLTNLIVNQMKKSYVTWNRNQVTDEIIIS
                     DLNQLSGGRLKITEGVKILEIRELLPQPRKKQQQGKTHGKQQNKQYGKKKGHNRH"
     gene            1171..2475
                     /gene="murA"
                     /locus_tag="PLZ75_10820"
     CDS             1171..2475
                     /gene="murA"
                     /locus_tag="PLZ75_10820"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOS72924.1"
                     /translation="MDTFIVQGGRSLKGEINPQGSKNEALQVICASLLTPEKVIIKNI
                     PDIADVNSLLDLLSCLGVRIGRKSGSVAVLQAADINLEYLRTDDFRIKSSGLRGSVMV
                     VGPLLARYGKAYMPKPGGDKIGRRRIDTHLNGFKKMGAEFDYDFSETCFKIGAPSLKG
                     TFILLDEASVTGTANLIMAAVLARGRTTLYNAACEPYIQQLCKMLVSMGALIRGIGSN
                     LLEIDGVRELKGCNHTILPDMIEIGSFVGMAAMTESEITIKNVSWENLGIIPDTFRKI
                     GIRLEKSEDDIFIPRQKHYEIQHFIDGSIMVIADAVWPGLTPDLLSVLLVTATQAKGS
                     VLIHQKMFESRLFFVDKLIDMGARIILCDPHRATVIGLDKSSRLRGTVMSSPDIRAGI
                     ALLIAAMSAEGESIIHNIRQIDRGYQSIDSRLNAIGACIRRI"
     gene            2571..3215
                     /locus_tag="PLZ75_10825"
     CDS             2571..3215
                     /locus_tag="PLZ75_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOS72925.1"
                     /translation="MSKKKSSLREEGKHTEKGQEFDNKEDNVNTESADPGAAGVSAAA
                     DQEEQPVAVDEDEPGTADKVVSSEEILEEKLAEMQDKYIRLSAEFDNYRKRTLREKMD
                     LTKFASENVLLKILPFMDDFERALVNMENTRDHNAVKEGIDLIYAKFLDFLKQNGVSE
                     VEALDTPFNIDLHDAVGKMPVEDKNRKGRVVEVIQKGYYLLDKVIRHSKVIVGE"
     gene            3271..4416
                     /gene="dnaJ"
                     /locus_tag="PLZ75_10830"
     CDS             3271..4416
                     /gene="dnaJ"
                     /locus_tag="PLZ75_10830"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOS72926.1"
                     /translation="MEKRDYYDVLGVPKNASKEDIKKAYRKQALKYHPDKNPGDKKAE
                     ENFKEAAEAYEVLSSDEKRSRYDRFGHAGMGGAAGGFPGGGMTIEDIFSSFGDIFGDA
                     FGGFGGFASGRRSRRVNKGTNLRVKVKLSLQEIANGTEKKIRVSKYVTCNTCGGEGAA
                     DSNSISTCQTCHGTGQVTRITNTLLGQMQTSSICPACGGDGKTITKKCPVCYGEGIVK
                     KDEIIKINIPAGVAKGMQMTVSGKGNAARRGGVNGDLLVLIEEEEHPELIREGNDLIY
                     NLFISIPDAILGTHVEIPTVENNVKIKIEPGTQPGKILRLRGKGLPEVNAYGRGDLLV
                     NVNVWIPKTMTREESKIIEKFKESGSFTPRPDKSDKGFFERMRGYFD"
     gene            4572..>4979
                     /locus_tag="PLZ75_10835"
     CDS             4572..>4979
                     /locus_tag="PLZ75_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS72927.1"
                     /translation="MALFEAVNITKQFGNITALNKVSISVPGKCIYGLLGPNGAGKTT
                     LLRIINQITGPDSGELYLNGKKLSPEDVRKIGYLPEERGLYKKMKVGEQALYFAQLKG
                     LSKHEAMRRLKYWFRKLEITDWWGRKVEELSKGM"
BASE COUNT         1553 a         1083 c         1238 g         1105 t
ORIGIN      
        1 ctactttttc tacaagggtg cagacctcat catcggttcc catcctcacg tcctgcaaaa
       61 gatggtctgg aaaaaagaca atcccgtttt caggaacaag gcaatagtct attccctcgg
      121 gaatttcgtt tcaaaccaga ggaaaccgct taccgacgga ggttcaatgg tgaggattga
      181 agtcgccaga agggacagca gcgttgtaat caccgatgca ggctacttcc tcacatgggt
      241 ctatactccc gttgaggacg ggaaaagaca gttctacatc cttccctgca gtgaattcga
      301 aaacagaccg gatttttttc atgacaggaa tgacttcctg caaatgcaga gattcataaa
      361 cacttcaaga tccttactga acaggcataa tgaaggcttc agggaactgt acgggaaaga
      421 agcggaataa tgatgcatca tggggtgcag cccgggcttt tattttattg acacgtaact
      481 tatttatttt aaattattag ctttgcgata aacagatttt ctgatgaact acgactacaa
      541 tacccagagg aaaaggatgg ccctgccgga atacggcaga aatgtccaga aaatggctga
      601 tcacatcaag accatcaagg acaggaatga gaggaaccgt gcagccagga cattaatcca
      661 gatcatgggt aatcttaatc ctcaccttcg cgatgaagga gatttcaagc acaaactgtg
      721 ggaccatctg gctcttattg cagattttga actggatatt gattcaccct atccccctcc
      781 ggaaagagga aagttcgccg aaaagccaaa tgagatacct taccggcagg gtgatataaa
      841 attcctgcac tatgggggaa taatagaaat aatgatcgat gccgcctgca aactggaaga
      901 cggtccggaa aaggaatatc tcacaaacct catcgtaaac cagatgaaaa aatcatatgt
      961 tacttggaac agaaaccagg taacagatga aataattatt tccgacctga accagttgtc
     1021 gggaggtaga ctgaaaataa ccgaaggcgt aaaaattctg gaaataagag aattgctgcc
     1081 ccagcccagg aaaaagcagc aacagggcaa gacacacgga aaacagcaaa acaagcagta
     1141 cggcaagaag aaaggtcaca accggcactg atggacactt ttattgttca gggaggccgt
     1201 tcccttaaag gtgaaataaa ccctcagggc tcaaagaatg aagctcttca ggtcatctgt
     1261 gccagcctgc tcacccctga aaaagttatc ataaaaaata ttcctgacat tgctgatgta
     1321 aacagcctcc ttgaccttct gtcatgcctt ggcgtcagga tcggcaggaa atccgggtcc
     1381 gttgccgtac tgcaggcagc ggatataaac cttgaatacc tccggactga cgatttcaga
     1441 ataaaaagtt ccggactgag aggatcggtg atggttgtcg gacctctgct ggcccggtac
     1501 ggcaaagcct acatgccaaa gccgggcggc gacaaaatag gccggcggag aattgatacc
     1561 catcttaacg gattcaagaa gatgggtgcc gaattcgatt atgatttttc agaaacctgt
     1621 ttcaaaatag gtgcgccctc acttaaggga acttttatac ttcttgatga agcttcggta
     1681 accggcacag ccaacctcat catggcagca gtgctggcca gaggccgcac aaccctctac
     1741 aatgcagcct gtgaaccata tatccagcag ctctgcaaaa tgctggtctc aatgggagcc
     1801 ctgatccggg gaatcggatc aaacctgctt gaaatagatg gcgtaaggga actgaaggga
     1861 tgcaatcaca ccattcttcc cgatatgata gaaatcggat ccttcgtcgg catggcagca
     1921 atgacggaat cagaaataac catcaaaaac gtatcctggg aaaacctcgg aataataccg
     1981 gatacattca gaaaaatagg tatcaggctt gaaaaatcgg aggacgatat tttcattccc
     2041 cgccaaaagc attatgagat tcagcatttc atcgacggat caataatggt cattgccgat
     2101 gcggtatggc ccggcctcac tcccgacctg ctgagcgttc tgcttgtaac tgccacccag
     2161 gcaaaaggat ctgtactgat acatcagaaa atgttcgaga gccggctttt ctttgtcgat
     2221 aagctgattg acatgggtgc acgtatcatt ctctgcgacc cgcacagggc cactgttatc
     2281 ggtcttgaca aaagcagcag gctgagagga acggtaatga gctcacccga tatcagggca
     2341 ggaatagccc tgctgatagc agccatgtcg gcggaaggag aaagtattat ccataatatc
     2401 aggcaaattg acaggggata tcagagtatc gactcccgcc ttaatgcaat aggagcctgt
     2461 atccggagaa tctgacctgt ccccggaata cccttctgcc aaattgtcaa ctctcctttc
     2521 atggcagatt atttgatggg ctttattgtc aaaatcgaaa tattattata atgagcaaga
     2581 agaaaagcag cctgagggag gaaggtaaac atacagaaaa aggacaagaa tttgataata
     2641 aggaagataa tgtaaacaca gaatccgcag atcctggtgc ggcaggtgta tctgcagctg
     2701 cggatcagga agagcagcct gtggcggttg atgaggatga acccgggaca gctgacaaag
     2761 ttgtatcttc agaagagatc ctggaagaaa aactggcaga gatgcaggat aaatatatcc
     2821 gtttgtcggc cgaatttgac aattacagaa aaaggaccct gcgtgagaaa atggatctga
     2881 caaaatttgc ttcagaaaat gttttactga agatacttcc ctttatggat gatttcgaga
     2941 gggctcttgt taacatggaa aacaccaggg atcacaatgc cgttaaggaa gggatagacc
     3001 ttatttatgc caaattcctg gattttctga agcagaacgg ggtttctgag gttgaggcgc
     3061 tcgatactcc tttcaatatt gatcttcacg atgcggtggg caagatgccg gttgaggata
     3121 aaaacaggaa aggcagggtt gttgaagtga ttcagaaggg ttattatctg ctggacaagg
     3181 tgataagaca ttcaaaagtg attgtggggg aataagattc gcccccgttg ccgctgacag
     3241 caggctgaag ttggaggaaa tctggaaaga atggagaaaa gagactatta cgatgttctg
     3301 ggagtgccga aaaatgcttc caaggaagat atcaagaaag cctacaggaa gcaggcactg
     3361 aaatatcatc ctgataagaa tcccggtgac aagaaagccg aggaaaattt caaggaagca
     3421 gcagaagcct atgaggttct gtcgagtgat gaaaaaagat cgagatacga cagattcgga
     3481 catgcgggta tgggaggtgc tgccggcgga ttcccgggag gagggatgac gatagaagat
     3541 atattttctt cattcggcga tattttcggt gatgctttcg gcggttttgg cggttttgcc
     3601 tcaggacgaa gaagccgcag agtcaataag ggtaccaatc tcagggtaaa ggtcaaactg
     3661 agcctgcagg aaatagccaa cggaaccgaa aagaagatca gggtgagcaa gtatgttacc
     3721 tgtaacacct gcggcggaga gggagctgcc gattcaaaca gtatttccac ctgccagacc
     3781 tgccatggca ccggacaggt aacaagaata accaatactc ttctcggcca gatgcaaaca
     3841 tcctccatct gtcccgcctg cggcggcgat ggcaagacaa tcacaaagaa atgccccgta
     3901 tgctatggcg aaggaatcgt caaaaaggac gagattataa aaatcaatat cccggcaggt
     3961 gtggcaaagg gaatgcagat gaccgtctcg ggaaagggaa atgctgccag gcgcggagga
     4021 gtcaacggcg acctgctggt actgattgag gaggaagaac atcccgaact gataagggaa
     4081 ggaaatgatc tcatatataa tctgtttata agtataccgg atgctatcct cggtacccac
     4141 gtagagatac ccaccgtgga gaataatgtc aagataaaaa tcgaaccggg aacccagccc
     4201 ggtaaaatac tccggctgag aggcaagggc cttccggagg ttaatgccta cggacggggc
     4261 gacctgctgg ttaatgtcaa tgtatggata ccaaaaacca tgacccggga agaatctaaa
     4321 atcatcgaga aattcaaaga atccggctca ttcactccca gacccgacaa gtccgataaa
     4381 ggattcttcg aaaggatgag aggatatttc gactagaaac accactcctt cctttatcgc
     4441 cgatccctta cactccgcca taatatccat tgcccggcat attacataat atcaacattt
     4501 ttcacatcgg cataaagatc cgcagagttt tttatattta catttccaaa tatctctaaa
     4561 ctattccttc aatggcatta tttgaagctg tgaacattac caagcagttc gggaatatca
     4621 ccgcactcaa caaggtaagt atttccgttc ccggaaaatg catatacgga ctgttgggtc
     4681 cgaacggtgc agggaaaacg actcttctca gaataatcaa ccagatcacc ggtcccgatt
     4741 ccggggaatt atacctgaac ggtaaaaaac tgagcccgga agacgtaagg aagatcggct
     4801 acctccccga ggaaagggga ttatacaaaa agatgaaagt aggggaacag gccctgtact
     4861 tcgcacagct caagggtctc tcaaagcatg aggccatgcg gaggctgaaa tactggttca
     4921 ggaaactgga gatcacagac tggtggggcc ggaaagtgga ggaactgtcc aagggaatg
//
LOCUS       DAOSPX010000246         4940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15501_length_4940_cov_5.510338, whole genome shotgun sequence.
ACCESSION   DAOSPX010000246 DAOSPX010000000
VERSION     DAOSPX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15501_length_4940_cov_5.510338"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PLZ75_10840"
     CDS             <1..770
                     /locus_tag="PLZ75_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HOS72928.1"
                     /translation="DEDLKLMNRRHTADQGREALDIIRKSGFDNVSADLIYGIPGMGT
                     ENWAANIDEILSFDIRHLSAYHLSIEPGTVFFRMKEKGQISEVDEDESTAEFNLLLDK
                     TGKAGFINYEISNFGKPGYFSIHNTNYWKQVPYLGLGPSAHSYNGYSRQWNISDVKNY
                     IRSVNAGKPLFEKEELDIRTKFNEYVMTSLRTMWGIDLLYVERIFEKEGYDYVVNLSG
                     KFIDYGLMRQDNNNLFLTSQGMMISDNIISEFIMPSP"
     gene            complement(777..1676)
                     /locus_tag="PLZ75_10845"
     CDS             complement(777..1676)
                     /locus_tag="PLZ75_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72929.1"
                     /translation="MADTNYQTDKTDKKVTPIGMIATTIILAAVLIFLIVMYFDQKHK
                     MVEMETVLTEEKDSLANELKNMAAAYDTMKTTNDTLNANLEKEKNRIIQLLAINASNA
                     QLIKKYRSEITTMREIMKSYIVQIDSLNTRNKMLTAENVEYRQQIARVRDTNVQLERE
                     RVALSSKVETASVIQAKNIVAAALNKKRKETNRINLMESLRVCFTLRENPIAPSGQKE
                     VFMRVIRPDSLVITGSPDNLFEYRGNKMIYSEKRMADYMNQDLEMCIFLSNTGDFITG
                     NYSIELYLDNSKIGSTTFMLAKK"
     gene            1809..3212
                     /locus_tag="PLZ75_10850"
     CDS             1809..3212
                     /locus_tag="PLZ75_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOS72930.1"
                     /translation="MSGFFGVVSNQDCVREVFYGTDYHSHLGTRRAGMVFIVPGKGFR
                     RSIHTLENSYFRTKFEDELKDFRGNVGMGIISDTDPQPILFTSHLGRFAIVTVSRLVN
                     IAELEAKILGRKKHFTENFEGTINPTEVIASLICEKDSFIEGIEYVFENVRGSCSLMI
                     MTEDGIIAARDKYGRTPVVIGRKDGACAIASESCSFPNTGFELEYFMGPGEIVRVSSE
                     GYTQLRKPGNTLQICAFLWVYYGYPPSYYEGINVDECRYRCGAALARRDSVEADFVCG
                     IPDSGVGHAIGYGNEKKIPNKRAYSKYTDTWPRSFLPSFQGQRNLVARMKLIPNRAVI
                     SGKKMIFIDDSIVRGTQLRHNTNDMRKYGAKELHMRIACPPILYPCDFLNFIESRTPM
                     ELAARRAVRELGGRDEELREYSDPDSEKYRKMVEKIAEKLDLDSLLYQRLDDLIAAIG
                     LPREKLCTHCWDGTGYF"
     gene            complement(3272..4051)
                     /locus_tag="PLZ75_10855"
     CDS             complement(3272..4051)
                     /locus_tag="PLZ75_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOS72931.1"
                     /translation="MAGGIYKTGLVLSGGGTRGFAHLGVLKALNEAGIYPDVVSGTSA
                     GALAGLMYCDGIGPEEAFKIMKGSRRLNYMRPTMPRDGLLQLSGIRKILEDHVTARRF
                     EDLKIPLYVSATDLNNGRPVYFSRGELLDVVIASASIPVLFRPVVINNIFYADGGILD
                     NMPVLPIENQCRLIIGSFVNPVGKEESFHNLIKITERTFMIMMSKEINEKSNKFNLFI
                     SPAELRNYGILSPEEAEAVFRIGYRATREKLRDSRILKLFS"
     gene            4317..4898
                     /locus_tag="PLZ75_10860"
     CDS             4317..4898
                     /locus_tag="PLZ75_10860"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS72932.1"
                     /translation="MSFTLEIGQKAVDFNLPATDGKKYSLSDFSSHKFLVVFFTCNHC
                     PYVTGSDEVTRATAEKYKSEGVVFAGINSNSEHTYEEDSFPNMVKRMEKHRFPWVYLH
                     DQDQSVAKAYGALRTPHFFVFDEERTLVYTGRGVDSPRDVSRMTVNDLENALEELVSG
                     RPVSVPLTNPIGCNVKWDGRDAHWMPPEACDLV"
BASE COUNT         1393 a         1007 c         1120 g         1420 t
ORIGIN      
        1 gggatgagga tctgaaactg atgaaccgcc ggcatacagc ggatcagggc agggaagcac
       61 tggatatcat ccgcaaatcg ggttttgaca atgtttcagc agatctgata tacggtattc
      121 ccggaatggg tacggaaaac tgggctgcaa atattgatga aattctttca tttgatatcc
      181 ggcatctttc ggcctatcat ctaagcattg agccgggtac ggtttttttc cggatgaagg
      241 aaaagggaca gatcagtgaa gttgatgaag atgaaagcac agcagaattt aatttattgc
      301 tggataaaac cgggaaggcc ggttttatta attatgaaat atcaaatttc ggaaaaccgg
      361 gatatttctc catacacaat accaattact ggaagcaggt tccctatctc ggactgggac
      421 cctcggcaca ttcatacaac ggatattcaa ggcaatggaa tatcagcgat gtgaaaaact
      481 acatccgctc ggtcaatgca ggcaaaccgc tttttgaaaa ggaagaactg gatatcagga
      541 caaagttcaa tgaatatgtg atgacatcac tgcggacaat gtgggggatc gatctgctct
      601 atgttgaaag gatctttgaa aaggaaggct atgactatgt tgtcaatctc agcggaaaat
      661 tcattgatta cggactgatg aggcaggata acaacaatct ctttcttacc agtcagggca
      721 tgatgatatc ggataatatt atttcagagt ttataatgcc ttctccctga tatctgttat
      781 tttttcgcaa gcatgaatgt tgtactgcct attttactat tgtccaaata gagttcaatg
      841 ctgtagtttc cggtaatgaa atcaccggta ttggacagga agatacacat ttcaagatcc
      901 tggttcatat aatcggccat tctcttttct gaataaatca ttttatttcc cctgtattcg
      961 aaaaggttgt caggacttcc tgtaataacc agcgaatcag gtcttataac cctcatgaac
     1021 acttcttttt gtccggaggg ggcaatggga ttttcacgga gagtaaaaca taccctgagt
     1081 gattccatca gattgattct gttggtttcc tttcttttct tgttcagggc tgctgctaca
     1141 atattcttcg cctggataac cgaggctgtc tctaccttgg atgaaagtgc caccctttct
     1201 ctttcgagct gtacatttgt atcacggact cgggctattt gctgcctgta ttccacattt
     1261 tctgccgtaa gcattttatt ccgggtgtta agggaatcta tctgaacaat atagctcttc
     1321 attatttctc tcatcgtggt gatctcgctt ctgtattttt ttatcagctg cgcatttgaa
     1381 gcatttattg ccagaagctg aatgatcctg ttcttttctt tttccagatt ggcattgagc
     1441 gtgtcattcg tggttttcat tgtatcatat gccgcagcca tattcttcaa ctcatttgcc
     1501 agggaatcct tttcttcagt aaggacggtt tccatttcaa ccattttatg cttctggtca
     1561 aaatacatca caataagaaa aatcagcacg gcggccagga ttattgttgt ggcgatcatc
     1621 ccgatgggag taaccttttt atcggtctta tctgtttgat aatttgtatc agccattgtt
     1681 aaatgagttt aatgaatttc aggtctgcaa aattataaaa aataataata acctgctata
     1741 taataaaatc cgaatcaatg attaatttta caaatgaaat aataattatt ctatttaaaa
     1801 tcaattatat gagtggtttt tttggtgtgg tttcaaatca ggattgtgta agggaagtat
     1861 tttatggcac tgattatcat tcccatctgg gtaccaggcg ggcgggaatg gtgtttatcg
     1921 tgcccgggaa aggcttccgg agatcaattc acacactgga gaattcatat ttcaggacta
     1981 agtttgaaga cgagctgaag gatttcaggg gcaatgtggg aatgggcata ataagtgata
     2041 cggatccgca acccattctt ttcacatctc acctggggcg ctttgcaata gtaaccgtaa
     2101 gccgcctggt gaacatcgca gaacttgaag ccaaaatact cggccgcaag aaacatttta
     2161 cagagaattt cgaaggcacc ataaatccca ctgaggttat tgcaagcctt atttgtgaaa
     2221 aggacagttt tatcgaggga atagaatatg tatttgaaaa tgtcaggggt tcatgttcac
     2281 tgatgattat gaccgaagac gggattattg ccgccaggga caagtacggg cggactccgg
     2341 tagttatagg gagaaaggac ggggcatgtg ccatcgcttc ggaatcctgc tcatttccga
     2401 ataccggctt tgaactggaa tatttcatgg gtccgggtga gattgtcagg gtcagttccg
     2461 aaggatacac acaactgaga aaacccggga acacattgca gatatgtgca tttctttggg
     2521 tttattacgg atatccccct tcgtactacg agggtataaa tgttgatgaa tgccgttaca
     2581 ggtgcggtgc agcccttgcc cgcagggact ccgttgaagc tgattttgta tgcggaatac
     2641 ccgattctgg tgtgggccac gcaataggat acggcaatga aaagaaaatc ccgaataaaa
     2701 gagcttattc aaaatatacc gatacctggc ccagaagctt cctgccctcg tttcagggcc
     2761 aaaggaacct tgttgccaga atgaagctga tacccaacag ggcagttatc agcgggaaaa
     2821 agatgatttt tattgatgac agtattgtca ggggaacgca gctcaggcat aatacgaacg
     2881 acatgagaaa gtatggtgca aaggaactgc atatgagaat agcatgtcct cccattctgt
     2941 atccctgtga ctttcttaat ttcatagagt cgagaacccc gatggagctg gctgcacgca
     3001 gggcggtaag ggaactggga ggaagggatg aggaactccg ggaatattcc gaccccgatt
     3061 cagaaaaata caggaaaatg gttgaaaaaa ttgccgaaaa acttgatctt gattctctct
     3121 tgtaccagcg gcttgatgat ctgattgctg caataggact tccccgggaa aaactctgta
     3181 cccactgctg ggacggaacg ggttattttt agttccaccg ggggaatacc ggcgaaacaa
     3241 gggctttatc caagaaagat gccgctgaaa ttcatgaaaa caatttcaga atccttgaat
     3301 ccctgagctt ttcccttgtt gccctgtagc ctattctgaa aacggcctct gcctcttccg
     3361 ggctgagtat cccgtaattc ctgagttccg cgggggaaat gaaaagattg aatttatttg
     3421 atttttcgtt gatttccttt gacatcatta tcatgaatgt cctttcggtt atcttaatca
     3481 gattatgaaa ggattcttcc tttcccaccg gatttacaaa tgacccgata atcagcctgc
     3541 actggttttc tatcggaagg accggcatat tgtcgagtat tccgccgtca gcataaaaaa
     3601 tattgtttat tacaacaggc ctgaaaagaa ccggaatgct tgcagatgca atcacaacat
     3661 ccagcagctc gcccctggaa aaataaaccg gcctgccatt gttcaggtcg gttgccgaaa
     3721 cgtaaagcgg aattttcaaa tcctcgaatc ttctggcagt cacatgatct tcaaggattt
     3781 ttcttattcc cgaaagctga aggagtccgt cacgcggcat agtgggtctc atgtagttaa
     3841 gacggcggct gcccttcatt attttaaatg cctcttcggg gccgatgccg tcgcagtaca
     3901 ttaatccggc aagagctccg gcacttgttc ccgaaaccac atcaggataa atgcctgcct
     3961 cgttaagagc cttcagtact ccgagatgag caaagccccg tgtgcctccc ccgctcaata
     4021 ccagacccgt cttgtaaatt ccgcctgcca taatggtaat cctttgaaat tccccttaaa
     4081 tttgctaaaa taattgattc aatttatgta atttctttat ttggctgtta tttcagtatt
     4141 gcaggtaaaa gttccggaag gagaagtata actgaacaaa ttcggtatga atattataac
     4201 atagcagaaa atcagggcgg ggagaaggtg taagggcctg ccgggaaaaa tttcatccgg
     4261 cacaataaac acttacgact aattattgtt agttttagaa caaattaaat ccatatatgt
     4321 catttacatt agaaattggt caaaaagccg tggatttcaa tctgccggca accgacggca
     4381 aaaaatacag cctgtcagat ttcagcagcc ataaatttct ggttgtcttt tttacctgca
     4441 accattgtcc ctatgtaacc ggttcggatg aagttacccg tgccactgct gaaaaatata
     4501 agtcagaagg ggtggttttt gcaggaataa actccaacag tgagcataca tatgaggaag
     4561 acagctttcc gaatatggtg aaaagaatgg aaaagcaccg ttttccctgg gtatatcttc
     4621 atgatcagga tcagtcagtg gcaaaggctt atggtgcatt gagaactcct catttctttg
     4681 tgtttgatga ggaaagaact cttgtatata cgggaagggg tgtcgatagt ccccgggatg
     4741 tttcaagaat gacggtcaac gacctggaaa atgcccttga ggaactggta tcaggcaggc
     4801 cggtaagtgt tccgctgacc aatcccatcg gatgcaatgt caagtgggat ggccgtgatg
     4861 cacactggat gcctccggaa gcctgtgacc ttgtatgatt tatttctgat ctttggaata
     4921 aattaactca taaaccaaat
//
LOCUS       DAOSPX010000247         4929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15545_length_4929_cov_4.003488, whole genome shotgun sequence.
ACCESSION   DAOSPX010000247 DAOSPX010000000
VERSION     DAOSPX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15545_length_4929_cov_4.003488"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..2497)
                     /locus_tag="PLZ75_10865"
     CDS             complement(146..2497)
                     /locus_tag="PLZ75_10865"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS72933.1"
                     /translation="MIRGRIGDVDGRPLSGASIGIEGTFRGTHSAEDGSYYLDGLKDG
                     IYRLRFSFIGYESQLISVELAGEVVLDVILQEKSFLAGEVIVNATRAGSRSPMAYTSV
                     DRETVSDRNSGLDLPFLLSLTPSLVETSESGNGIGYTSLRIRGTDASRINVTVDGIPA
                     NDSESQQVFWVNLPDMASSVDNIQVQRGAGTSSNGSGAFGATLNIQTTTAGNEPFAQI
                     GTSLGSFNTSRKMIAAGTGLLADRFAFGLRLSDVKSDGYVDRTGSDHRSAFINGILRG
                     TRSSLKAGIILGEEHTGIGWVGVPAEMLSVNRKYNPAGEYTDMNGVVRYYDNESDNYR
                     QNHYRIVYDARRGENFSFSSALHYTHGTGYYEQYRENARLSSYGLPEISLNGSVITRS
                     DLVRRKWMKNDFYGLVSSFRYRNEKLEAVLGGGASHYSGDHYGRLIWMRHTGDLEKDH
                     QWYLNSGIKREFSIYGKIDYSLTGKLSFFADLQYRHINYRMKGIDDDLKDLDQKHYFR
                     FFNPKTGLFYSIDRKQDAYLSFSVARREPTRTDFKEAADDPRATPKHETLLDTELGYK
                     RRAERYSVAANMYAMFYRDQLVPTGELSSTGRSIMTNVPKSHRLGIELISALKPAAFF
                     NWDFSITLSRNKITGFTGYYIDYNTSDWSEEYKSKDLGTVDIAYSPSLTGVSDIEFRV
                     FKNTDLHFISKYVGKQYFDNTMNSERMIDPYFVNNFRIDSEPVVRGLKNTRFQFMLNN
                     IFNSMYVSNAYGGSWYEDGIEKTWAYYFPQAGINFMLRAEVLF"
     regulatory      complement(2649..2742)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2821..3210
                     /locus_tag="PLZ75_10870"
     CDS             2821..3210
                     /locus_tag="PLZ75_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72934.1"
                     /translation="MLSKLNYKGHNRIAIINAGDKFLEEVSDKHGGVIIDTEIDQRCP
                     YNFMIIFVTTIREVSDISPVALHNLTADGTLWFCYPKKYSKKFSTEPERYNVWKSIDD
                     LGFHGTRKVSLDENWSAIRFRNKKHIK"
     gene            3332..3862
                     /locus_tag="PLZ75_10875"
     CDS             3332..3862
                     /locus_tag="PLZ75_10875"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009293029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOS72935.1"
                     /translation="MIKTTRIRTPLGEMLAGATEEGICILEFADCMKVSGELRDLKKL
                     LNAEIKQGRNIHLRHLKKQLKEYFAGRRTEFDLPLLTPGTKFQQAVWNELLKIPYGST
                     ISYQQQADALNNPLAVRAVAGANGSNRIGIVIPCHRVIGSDGSLVGYGGGVRRKKWLL
                     DHEKKYSGQPVTMQLF"
     gene            complement(3873..4511)
                     /locus_tag="PLZ75_10880"
     CDS             complement(3873..4511)
                     /locus_tag="PLZ75_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOS72936.1"
                     /translation="MEIIQAVVDWYMVNLNYFTVALLMAIESSFIPFPSEVVIPFAAY
                     KAAQGGLNVFGVVIAGTIGALIGALFNYYLSLYLGRPLVYKFADSRLGRVLLLSKDKV
                     QHAENYFIRNGNSSTFIGRLVPAVRQLISIPAGLARMDMRNFLLYTAAGAGIWNIILA
                     VVGYFVYELRDKILPYIGHLLIVLGVAFIAYLIIRGKLNKKKNKDQNAEFGQ"
     gene            complement(4641..>4929)
                     /locus_tag="PLZ75_10885"
     CDS             complement(4641..>4929)
                     /locus_tag="PLZ75_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS72937.1"
                     /translation="GLPLEGHTWLDRDNWGYRTFQDADTLFKTYSAYLGQMVPFIKRG
                     LSAAIYTQTTDVEIETNGLLTYDRKVMKVPAEKLRAAARKMFDAAKGILFR"
BASE COUNT         1347 a         1203 c         1088 g         1291 t
ORIGIN      
        1 ccgaaatcct gtgatcaggg caataaagcc ggggaaaaat gatctgacaa ataaaatata
       61 gcggatgcag tatgctgaat gcagttccga accgggcagt ggtttttcgt gagtcttgct
      121 ggtgaaataa gggcttccgg cattttcaga acagtacttc ggcccttaac atgaaattta
      181 ttcctgcctg agggaaataa taagcccatg tcttttctat cccgtcttca taccagctgc
      241 cgccgtaggc attgctcacg tacatgctgt tgaaaatatt gttcagcatg aactggaaac
      301 gcgtgttttt caatcccctt accacaggtt cggagtcaat acggaaatta ttgacgaaat
      361 acggatctat catcctttcg ctgttcatgg tattgtcaaa atattgcttc cccacatact
      421 tgcttataaa atgcagatca gtatttttaa aaactctgaa ttctatgtca ctgacgcctg
      481 taagggaagg ggaatatgct atatctaccg tccccagatc cttgctttta tattcctccg
      541 accagtcaga tgtattataa tcaatatagt acccggtaaa tccagtaatc ttattccggc
      601 tcagggtaat gctgaaatcc cagttgaaaa atgcagcagg tttcagtgca gatatcagtt
      661 caatacccag gcggtggctt ttagggacat tcgtcatgat tgaacggccc gtgctgctca
      721 gttcaccggt cggcacgagc tggtccctgt aaaacattgc atacatattg gccgccacgg
      781 aatatctttc agcccttctc ttgtatccga gctctgtgtc aagcagtgtt tcatgttttg
      841 gtgtggcccg cggatcatcc gcggcttcct tgaaatcggt tctcgtaggt tcacgcctgg
      901 ccacggaaaa ggaaagatat gcatcctgtt tccggtcaat ggaatagaac agacccgttt
      961 tgggattaaa gaaacggaaa taatgtttct ggtccagatc cttcagatca tcatcgatgc
     1021 ccttcatcct gtaattgata tgcctgtact gcaggtcagc aaagaaggaa agcttcccgg
     1081 ttagcgagta gtcaatcttt ccgtagatgc tgaattccct cttgataccg ctgttcagat
     1141 accactgatg atccttctcc aggtctcctg tatgcctcat ccagatcagc cgtccgtaat
     1201 ggtcacccga atagtggctg gctccgcctc cgagaacagc ctccagtttt tcattcctgt
     1261 acctgaagga actgaccagg ccatagaaat cgttcttcat ccatttcctt ctgacgaggt
     1321 cgctcctggt aattactgac ccgttcagac ttatctccgg gaggccgtag gaggaaaggc
     1381 gggcgttttc cctgtactgc tcataatagc ctgtgccatg ggtataatga agtgcggaac
     1441 tgaaactgaa gttttcaccc cgccttgcat cataaacaat acggtaatgg ttctgcctgt
     1501 aattatcact ttcattgtca taatagcgta caacaccgtt catgtcggta tattcccccg
     1561 ccgggttata tttcctgtta acggaaagca tttctgcagg aaccccaacc catccgatgc
     1621 cggtatgctc ctccccgagg atgataccgg ctttcagact ggaacgtgtg ccgcgcaata
     1681 tgccgtttat aaatgctgag cggtgatcgg agcccgtcct gtcgacatat ccgtcacttt
     1741 tcacatccga aagcctcagc ccgaatgcaa atctgtctgc aagcaatcct gtacctgcag
     1801 caatcatttt tcttgaggta ttgaaagagc ccagcgatgt gcctatctgt gcaaatggtt
     1861 catttcccgc ggttgtggtc tgtatattaa gggttgcccc gaatgctccg gaaccgtttg
     1921 atgaagttcc tgctcctctc tgtacctgta tattatcaac agaggatgcc atgtcaggca
     1981 gattaaccca gaacacctgc tgcgattccg aatcatttgc aggaatgccg tcaacagtga
     2041 catttatcct gcttgcatcg gttccccgta ttctgagact ggtataacca atcccgttgc
     2101 ctgattcgga tgtttcaacc agggaaggag tcagactcag caggaaaggc aggtcaagac
     2161 cgctgttccg gtccgatacg gtttccctgt caaccgaagt atatgccatg ggcgaacgtg
     2221 aacctgccct ggtagcattc acaatgacct ctccggccag aaaggatttt tcctgaagaa
     2281 tcacatccag aacaacttca ccggccagtt caacgcttat aagctgtgat tcataaccga
     2341 tgaatgaaaa cctcagcctg taaataccat ctttcagtcc atcaagataa taagaaccat
     2401 cttcagcgct atgtgttccc ctgaatgtac cttcaatgcc tatggatgca cctgacagtg
     2461 gcctgccgtc aacgtcacct atcctcccgc ggatcacgga actgctttcc tggaaagcaa
     2521 atgactgccg gaaaacaaaa atgcacgcaa aaaaaatcaa aatacacttt agcctcatct
     2581 ttcaacatat ttttctgctt aaactctctg cctgccgaat atgaggtttt aattttcagg
     2641 ctgccgtgaa ttgtgagttt gcttccctcc gccggcatta tccggatcag gtgtaagggt
     2701 atgatctcag cccgtagtat taaggcaccc cattttcaga taacacaaag atacagcctt
     2761 tttttctctg attcaatttt ttcttaattt agctgttcct atgattctct gacaggaaat
     2821 atgcttagca aactcaatta taaaggacat aacaggatag ccataataaa tgccggggac
     2881 aagtttcttg aagaagtatc agataagcac gggggagtaa ttatagatac tgaaatagat
     2941 cagagatgcc cttataattt catgataatc tttgtcacca ccatcaggga agtcagtgac
     3001 atctctcctg tggccctgca taatctgact gcagacggga ctttgtggtt ctgctatccg
     3061 aaaaaatact caaaaaagtt ttcgacggaa cctgagcgct acaatgtctg gaaatcaatt
     3121 gatgatttgg gatttcacgg gacaagaaag gtatctctcg atgaaaactg gtcggcaata
     3181 aggttccgca acaaaaaaca catcaaataa gccctggaca gcccctgaaa cagtaaatga
     3241 aggtgagctg cgggcttgaa gggtgccgga gggaacaggg gattgcactg agaaccggat
     3301 cagtgaagcg gaacacatta atatgcaggt catgataaaa actaccagaa tcaggactcc
     3361 actgggcgaa atgcttgcag gtgccacgga ggaaggaatt tgcattcttg aatttgcgga
     3421 ttgcatgaag gtatccggag aactcaggga tctgaaaaag cttctgaatg cagaaatcaa
     3481 acagggccgc aacattcatc tgagacatct gaaaaaacag ctgaaggaat attttgccgg
     3541 caggagaaca gaattcgatc ttccccttct gacgcccgga acaaagttcc agcaggctgt
     3601 atggaatgaa ctcctgaaaa tcccctatgg ctcaaccatc tcttaccagc agcaggctga
     3661 tgccctgaac aatcccctgg cagtcagggc agtggccgga gccaacggat caaacaggat
     3721 aggaatcgtg attccctgcc acagggtaat cggctccgac ggcagcctgg taggctacgg
     3781 tggcggtgtg agaagaaaaa aatggcttct cgaccacgaa aaaaaatact cgggtcagcc
     3841 tgtaacgatg caactttttt aaaatgctgc ccttactgcc caaactctgc gttttggtct
     3901 ttgtttttct tcttgttcag ttttcccctg attataagat aggcaatgaa ggcaacgccc
     3961 agaacgatca gcagatgacc gatatacggc aggattttat cccgaagttc atatacaaaa
     4021 taaccgacaa cggccagtat gatattccag attcccgccc ctgcagcggt atacagcagg
     4081 aaattacgca tatccatccg cgcaaggcct gcaggtattg aaataagctg ccgcaccgca
     4141 ggaacaagcc ttcctatgaa agtggatgaa ttaccgttcc ttataaaata gttttccgca
     4201 tgctgcacct tatccttcga aagaagtaac acccgcccga gtcttgaatc tgcaaatttg
     4261 taaaccaggg gtctgccgag ataaagtgaa aggtaatagt taaaaagcgc tcctatcaat
     4321 gccccgattg ttccggcaat gaccacgccg aaaacattca gtccgccctg tgccgccttg
     4381 taggcagcaa agggaataac cacttcggag ggaaagggaa tgaaggaact ttctatggcc
     4441 ataagcaggg ccacggtaaa atagttcagg ttgaccatgt accagtcaac aactgcttga
     4501 attatttcca tattgaatca aaaaacaagg ctgcaaatat agcaatattg cccggtcctc
     4561 aatcagtctt ccgcgaaatc ggtgacaatc tgatttgtca gtgccgactc atcctgataa
     4621 caggaataat tatttcccgc ttacctgaac aaaattccct ttgcggcatc aaacattttc
     4681 cttgcagccg ctctcagctt ttctgcagga accttcatca ccttcctgtc ataggtgaga
     4741 agaccgttgg tttctatctc aacatcagtg gtctgggtat atattgccgc tgaaagccct
     4801 ctttttataa acggaaccat ctgcccgagg taagcggaat atgttttgaa aagggtatcc
     4861 gcatcctgaa aagtcctgta tccccagttg tcacggtcga gccaggtatg tccctcgaga
     4921 ggaagtccc
//
LOCUS       DAOSPX010000248         4918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15596_length_4918_cov_3.494139, whole genome shotgun sequence.
ACCESSION   DAOSPX010000248 DAOSPX010000000
VERSION     DAOSPX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15596_length_4918_cov_3.494139"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLZ75_10890"
     CDS             complement(<1..770)
                     /locus_tag="PLZ75_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOS72938.1"
                     /translation="MTNNIILEKLDGVKRRFVEVGELLTRQEVLSDMGRYIALNREYK
                     ELQPIIEAYEQYRLALSNLASVREMLVAEKDEEMREMAKSEYEQLNEQIPEMEEELKI
                     LLLPGDPEDEKNAIIEIRAGTGGDEASIFAGDLLRMYTRFFEMKGWKAEIIRYTEGTA
                     GGYKEIVMSVSGEGVYGIMKYESGVHRVQRVPQTETQGRVHTSAASVVVLPEAGEFDV
                     EIKNEDVRKDTFCSSGPGGQSVNTTYSAIRLTHVPTGIV"
     gene            complement(859..2034)
                     /locus_tag="PLZ75_10895"
     CDS             complement(859..2034)
                     /locus_tag="PLZ75_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOS72939.1"
                     /translation="MARKSKYDLRGVSSQKEDVHAAIRNIDRGLFPKAFCKIVPDILG
                     GDPEYCNVMHADGAGTKSSLAYVYWKETGDMSVWKGIARDAIVMNIDDLLCIGAADNI
                     LVSSTIGRNRNLVPGEVISAIIGGMEEVLEDLRSMGIGIWSTGGETADLGDLVRTIVV
                     DSTVIARMKRADVISNHNIRPGDVIVGLASYGRTSYEREYNGGMGSNGLTSARHDVFG
                     SYLAERYPESYDSGMPENLFYTGRLKLTDPVEGSTLNAGKLVLSPTRTYAPVIRELLE
                     SMRHEIHGMIHCTGGGQTKILHFVDNMHVVKDNLFPLPPLFRMIREQGGTEWREMYQV
                     FNMGHRFEIYTGQKNADKIISTASAFGLDARIIGHCKASDTKKLTIVSESGRYEYSS"
     gene            complement(2053..4053)
                     /locus_tag="PLZ75_10900"
     CDS             complement(2053..4053)
                     /locus_tag="PLZ75_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOS72940.1"
                     /translation="MRRFTALYILLVLFAFQDSAAQKRRHERAYDAYNAGEYYDAIDE
                     FKNTYSKTKKSDKESRAEYIFMIAECYRRVNDPRNAETWYKQAVRSAFSRPEAQFWLA
                     STLKKNGKYQQAIEEFRTYKQVAPADARADQEIRACELALEWQRNPEPYIVDELKDLN
                     SRFADFSPAYGRDDFGVVYFTSSRDGATGNKTHGATGQGFTDIFVSRLDKKSKWSIPV
                     PLDVVNSEFEDGTPCVISDYREMYFTRCEAGKREKKGCVIMYSKRTGDSWSKPEKLDI
                     LPDTLVTAHPALSSDGLTLYFVSDMPGGFGGKDIWKVTRTGEGQSWSDPVNLGPDINT
                     AGDELFPYLRDETTLYFSSDGHIGMGGLDIFRANAQPDGSWSVQNMKAPINSPADDFG
                     IVFEDGNEKGMFSSTRKGRGNDELYSFELPPLKFNVTGLVKDEKTGAPIQGSTIQLIA
                     SDGSNLQAETGEGGDFRFALKADVDYIFLASKKGFLNGKEKETTKGQEKSRDFMVTIL
                     LTAIDRPVELPNIFYDFGKWDLRPESMVSLDKLVETLNDNPNITIELMSHTDSRDTEE
                     YNLELSQKRAQSVVDYLIGKDIDPARLSAKGYGESSPKVVDEEISEQSPFLKPGTLLS
                     EQYIESLPSEEQREIAHQINRRTEFRVLRTDYVPAGEPAKDK"
     gene            complement(4195..>4918)
                     /locus_tag="PLZ75_10905"
     CDS             complement(4195..>4918)
                     /locus_tag="PLZ75_10905"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HOS72941.1"
                     /translation="NAAVAYQLRNFKKEVDHIIKQGRNKDEAIFQVIKKYILASRKVL
                     FEGDNYSREWHEEAEKRDLCNIKSIPASIRCYMDPEGSRIFTESGIFTGKELESRCEV
                     EYEKFTKKIQIEARVLGDLAINHIVPTAITYMNRLLDNVKGLKEVFGDGEYERMAAAD
                     REMIITISGHISEIREKVLQMIEERKKANVVEDAYRKSLMYEEKVKPYLDEIRYHIDR
                     LELVIDDEIWPLPKYRELLFTR"
BASE COUNT         1149 a         1336 c         1003 g         1430 t
ORIGIN      
        1 actattcccg tcgggacatg agtgagcctt atggcggaat aggttgtatt gaccgattgt
       61 ccgccgggac ctgatgaaca gaatgtatct ttcctcacat cctcgttctt tatttcaaca
      121 tcaaattccc cggcttcggg aagcaccact accgatgctg ctgatgtatg aacccttccc
      181 tgggtttcag tctggggcac acgctgaacc ctgtgtactc ccgattcata tttcattata
      241 ccatacaccc cttctccgga aactgacatc actatctcct tgtagccccc ggcggttcct
      301 tcggtataac ggatgatttc agctttccag cccttcattt caaagaaccg ggtatacatc
      361 cgcaggagat cgccggcaaa gatgcttgcc tcatcccctc ctgttcctgc ccttatctca
      421 atgatcgcat tcttttcatc ctccggatca cctggaagaa gaagtatttt cagttcttcc
      481 tccatttcag gaatctgctc attcagctgt tcgtattcac tcttagccat ttcccgcatc
      541 tcctcatcct tctcggcaac aagcatttcc ctgacacttg caagatttga caaggcaagc
      601 ctgtactgtt cgtaagcctc aatgatgggc tgaagttcct tgtattccct gttcagagct
      661 atatagcgcc ccatgtctga aagcacctcc tgacgggtca gaagttcacc aacctcgaca
      721 aaacgccgct tgacaccatc gagcttttca agaataatat tattcgtcat gggtttttta
      781 tttcagggtg caaatataat aaaaagttaa atagccgcaa tggctccggg ataatgcaga
      841 tacctaagaa cgaggaaact atgaggaata ttcatatctg ccgctttcag acacaattgt
      901 cagttttttc gtgtcagaag ctttacaatg gcctatgatt cgggcatcca gaccgaaagc
      961 ggatgccgtg ctgattattt tatctgcatt cttctgcccg gtatagattt cgaaccggtg
     1021 acccatgttg aagacctgat acatttccct ccactctgtt ccgccctgtt ccctgatcat
     1081 cctgaaaagc gggggaaggg ggaaaagatt gtcctttaca acatgcatat tatcaacaaa
     1141 atgcagaatt ttagtctgcc ctccgcccgt acagtgaatc attccgtgga tctcatggcg
     1201 catggactcc agcagttccc gtattacggg tgcataggtc ctggttggtg aaaggaccag
     1261 ttttccggca tttaatgtgc tgccttcaac cggatcggtc agcttcagcc ttcctgtata
     1321 aaaaagattc tcaggcattc ccgaatcata gctttccggg tacctttcag ccagatatga
     1381 tccgaaaaca tcatggcgcg ccgaagtaag gccattgctt cccattcccc cgttgtattc
     1441 cctttcatag cttgtccggc cgtatgaggc cagacctaca atgacatccc cgggccggat
     1501 attgtgattc gagatcacat ccgccctttt catccgggca ataaccgttg aatccacaac
     1561 aattgtccgg accagatccc ccaggtcagc cgtctccccg cctgttgacc atattcctat
     1621 tcccattgac cgcaggtcct ccagcacttc ttccatcccg ccgatgatgg cggaaatcac
     1681 ttcgcccggc accaggttcc tgttccttcc tatcgtggag gaaacaagta tgttgtcggc
     1741 agctcctatg cacaggagat catcaatatt cattacaatg gcatcccggg ctattccctt
     1801 ccagactgac atatcgcctg tttccttcca gtaaacatag gcaagcgatg attttgtccc
     1861 cgccccgtcg gcatgcatca cattgcagta ttccggatcc cccccgagaa tatcgggtac
     1921 tattttacag aaagctttcg gaaatagtcc cctgtcgatg ttccttatag ccgcgtgaac
     1981 atcttccttc tgtgaggaaa cgccccgcag atcgtatttc gatttcctgg ccattttccg
     2041 gaattgtcag tttcacttat cctttgccgg ttctcctgcc ggcacataat ccgtcctcag
     2101 caccctgaat tcggtccggc ggtttatctg atgggctatt tccctttgct cctcgcttgg
     2161 aagcgactca atatactgtt cggagagaag tgtgccaggc ttcaggaaag gtgactgttc
     2221 agagatttcc tcatccacca ctttaggact tgattccccg tatccctttg ccgagagcct
     2281 tgccggatcg atgtccttgc ctataagata gtcaacaacc gactgggcgc gtttttgcga
     2341 aagctcaagg ttgtattcct ccgtatcgcg cgaatcggta tgcgacatca gttcaattgt
     2401 gatattcgga ttatcattga gtgtttcaac cagtttgtca agcgacacca ttgattcggg
     2461 cctgagatcc catttgccga aatcgtagaa aatgttcgga agttccacgg gccggtcaat
     2521 tgcggtaaga agaatggtaa ccatgaaatc gcggcttttc tcctgtccct tggttgtctc
     2581 tttttccttt ccgttcagga atcctttttt ggatgccagg aagatgtaat ccacatctgc
     2641 cttcagggca aatctgaaat cgccgccttc acccgtttcg gcctgaaggt tggaaccgtc
     2701 actggcgatg agctgaattg ttgatccctg gatcggagca cccgtctttt catcctttac
     2761 cagtccggtg acattgaatt tcagcggagg cagttcaaat gaatagagtt catcatttcc
     2821 cctgcccttg cgggttgaac tgaacatccc cttttcgttc ccgtcttcaa agacaattcc
     2881 gaaatcatca gccggactgt ttatgggggc tttcatgttc tgaaccgacc atgaaccgtc
     2941 aggctgggca tttgccctga atatgtccag acctcccatc cctatatggc cgtcagatga
     3001 aaagtaaagc gttgtctcat ccctcaggta aggaaaaagt tcatctccgg ctgtgtttat
     3061 atccggcccc aggttaaccg gatccgacca cgactgtcct tccccggtac gggttacttt
     3121 ccatatatcc ttcccgccaa atcccccggg catgtccgat acaaaataaa gtgtcagccc
     3181 gtcggacgaa agggccggat gtgcagtcac aagagtatcg ggcagaatat ccagcttctc
     3241 cggcttgctc cagctgtctc ctgttctttt tgaatacatt atcacacaac cctttttctc
     3301 acgcttgccc gcctcgcacc tggtaaaata catctccctg taatcggata taacacaggg
     3361 cgtgccgtct tcaaattcac tgttaacaac atcaagaggc acgggaatgc tccacttgga
     3421 tttcttgtcg agacggctta caaaaatatc ggtaaatccc tgtcctgtcg ctccgtgtgt
     3481 tttgttaccc gttgcaccat cccttgatga ggtaaaataa acaaccccaa aatcatccct
     3541 gccgtatgcc ggactgaaat ctgcaaaacg ggaattaagg tctttcagtt catcaacaat
     3601 atatggttcc ggatttctct gccattcaag ggcaagctca caggccctta tctcctggtc
     3661 agccctggca tcagccgggg caacctgctt gtaagttctg aattcttcaa tggcctgctg
     3721 gtatttgcca ttctttttca atgttgatgc cagccagaac tgggcctcgg gtcttgaaaa
     3781 agccgacctg acggcctgct tataccatgt ttcggcattc cggggatcat tgaccctgcg
     3841 gtaacactcg gcaatcatga agatatattc tgcccttgac tccttgtcag acttttttgt
     3901 cttggaatat gtattcttga attcatcgat ggcatcataa tattcacctg cattgtatgc
     3961 atcataggcc cgttcatgcc ttctcttctg cgcggcagaa tcctggaatg caaacagtac
     4021 aagcaagata taaagggcag taaaccttct catgatatcc gtagcgattt gaccataaaa
     4081 ataattaaat taaccagacg gatgttaatt acaaacggca ggggcgaaca attattataa
     4141 tttcccggcg tgcccggatc cggccgccat aaaacgaagc ttctggcacc aagcttatct
     4201 tgtgaaaagc agttccctgt atttgggaag aggccagatt tcgtcatcaa taacaagttc
     4261 cagcctgtcg atgtgatatc ttatctcatc aagataaggt ttgacctttt cctcgtacat
     4321 caatgacttc cggtatgcgt cctctacaac attggccttt ttcctttcct ctatcatctg
     4381 aagtaccttt tcccttattt ctgagatatg gccggaaata gtgataatca tttccctgtc
     4441 ggcagcagcc atcctttcat attcgccgtc gccgaatacc tccttcaggc ctttaacatt
     4501 atccagcagc ctgttcatat aagttatggc agtcggcact atatggttta ttgccagatc
     4561 acccagcacc cttgcctcaa tctgtatctt tttcgtgaat ttttcatatt caacctcaca
     4621 gcggctttct agttcctttc cggtaaaaat gccggattcc gtaaatatcc tgctcccttc
     4681 cggatccata tagcatctga ttgaagcagg tatgctttta atattgcaca aatccctttt
     4741 ttctgcttct tcatgccatt ccctgctgta gttgtcacct tcaaacagga ctttccgcga
     4801 agccaggata tattttttga taacctgaaa aatagcctca tccttgtttc ttccctgttt
     4861 tataatatga tcaacctctt ttttgaaatt cctgagctgg tatgccaccg cagcatta
//
LOCUS       DAOSPX010000249         4900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15663_length_4900_cov_4.257792, whole genome shotgun sequence.
ACCESSION   DAOSPX010000249 DAOSPX010000000
VERSION     DAOSPX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15663_length_4900_cov_4.257792"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLZ75_10910"
     CDS             complement(<1..763)
                     /locus_tag="PLZ75_10910"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOS72942.1"
                     /translation="MNKELNFTGNIIMIGCGSIGQAILPLLGRHLDGIYNRLTVLSAN
                     ESGRRMAQASGAKFIHSIFTPGNYRTILKEYVGEGDLLLNLSIGVSSLDLIRFCAEQG
                     ALYVDTSIEPWPGVFGDPMLELHERTNYIIRSKALDLAKELGPDSPTAVIDHGANPGL
                     VSHFVKRALADLNQIIRKENTMPSSREGWADLARDLGITTIQISERDTQASHKPKRQG
                     EFINTWSIDGFVDELMQPAELSIGTSEIRQLRVPSC"
     gene            1146..2318
                     /locus_tag="PLZ75_10915"
     CDS             1146..2318
                     /locus_tag="PLZ75_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72943.1"
                     /translation="MKKRKSSVLIFFIVLVLLNFLNVIPGNSQKASDHAEIGRYGTSS
                     ISYQELANFNEYKLALGPVRNDSVSEILKIQGKVMMTLYQGPADASSYEIYHAYRDLL
                     ISKQYDILYSCSGTECGTAFLSPLYKLSAFAYDHGYNNSAPITQGSQDFSYLITARKS
                     TGEKTTYVSLIVSHGWFKYPVYKLDVIEMQKPGGKISSIQAAKEDVPSDRTVPDRSGT
                     LHEKVAGRPAEAVSPAMTPDRSPERDEDAGAVRFGFHMSADSYFGLLLRARHFEFVIK
                     GYADLFDGFPEGYRPDDFLMIGGHISYLFRPWEKADIGVGLDLRQGISITGNIKYVQY
                     IDAGPVLSFNYSPGKHFVISGLFYPVWLNTRETDMDDSFSLAVQIPRASVALGFLF"
     gene            2461..3675
                     /locus_tag="PLZ75_10920"
     CDS             2461..3675
                     /locus_tag="PLZ75_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOS72944.1"
                     /translation="MKNLILNFSVLTILICFYGCSSDVQSNKKKISPPGNDYYVVAYI
                     WPSCHDDPLGRKMLWPEGTGEWEVIKKGNPRYEGHYQPRLPLWGHEPDNDPKVVERWI
                     DVATDHGINVFVYDWYWYDGGPFLESALNDGFLGAVNNEKMQFYIMWANHDVKYNYWN
                     YHLHKDNTDILWDAKVDRENFRIIVDRVINQYFKRPNYFKINGEPVFSIFSIGKLVES
                     FDNSLEEARKGLEYFRDEVRKAGFPGLHIQWNQGGGSIMSEKAAGNFVNSVRTMGFNS
                     VAMYNMGGLDEDYLVYGANSLKIREQMDSILDVPVFPCVSVGWDDTPRFPDKGIHDVV
                     HYHNTPQSFAALLAKAKKYADSHPEQPRLITINAWNEWVEGSYLLPDMLHGFGYLEAV
                     REVILEGKYDKY"
     gene            3716..4612
                     /locus_tag="PLZ75_10925"
     CDS             3716..4612
                     /locus_tag="PLZ75_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS72945.1"
                     /translation="MEKKETGSSRREVLKKLAATSVVILTGGKMKAETLKDRQAPEAG
                     LKGNINHSACRWCYSSIPLEEFCRGAKEIGLKAIDLTGPDEWPVLKKYGLDSSMCNGA
                     EIDISRGWNDSRYHSTLVKNYSEMIPLVAKAGYKNLICLSGNRDGKDDETGLRNCVEG
                     LKQVLGLAEKHGVMLVMELLNSRVDHPDYICDRTWWGVELVKRAGSENLKLLYDIYHM
                     QIMEGDIIRTIRDNHQHIAHYHTGGVPGRNEIDDSQELYYPAIMRAIVETGFKGYVAH
                     EFVPSRDDKLESLRQAVAICDV"
BASE COUNT         1374 a          983 c         1206 g         1337 t
ORIGIN      
        1 cgcagctggg taccctcaat tggcggattt cagaagtacc gatggaaagt tctgccggct
       61 gcatgagttc atctacaaaa ccatctattg accatgtatt gatgaattcg ccctgccgct
      121 tgggtttatg actggcctgg gtgtcccgct cagatatctg aatagtggtt atcccgagat
      181 cgcgggcaag atctgcccat ccctcccgtg aagaaggcat tgtattttct ttccggatta
      241 tctgattcag gtcggcaagc gcccgtttga caaaatgtga tacgagcccc gggtttgcac
      301 catggtcaat gactgctgtg ggtgaatcag gtccgagttc tttcgcaaga tccagagctt
      361 tggaacggat aatgtagttt gtgcgttcat ggagttcgag catggggtcc ccgaaaactc
      421 ccggccatgg ttctattgaa gtgtcgacat ataatgcacc ctgttctgca caaaagcgga
      481 tcagatcgag gctggacaca ccgattgaca ggttcagaag cagatctccc tcaccgacat
      541 attctttcag gattgttctg taattacccg gtgtaaagat gctgtgaata aactttgctc
      601 cggaagcctg ggccattctt cgtccggact cattggccga gagcactgtc agccgattat
      661 agattccgtc aaggtgccgg ccgagaagtg gcagaattgc ctgtccgatg ctgccgcatc
      721 ctatcattat gatattgccg gtgaaattca gttctttgtt cattttatcc cagtttgtgt
      781 ttatgttcat tgattcagta ctaagctgga aaacccgttg ctgcgggctg attccctgga
      841 tgctgcccga aatgtgatgg agataaatta ccgtggtttt gcttaatctg ttaagctgaa
      901 aacagtattt ccgtcagggg aaaatactaa gaaataattt aaacagcaac ctgttgttcc
      961 ggatggctca tttacaagcc ataacaaaat tgcctgtgcc gaattaaatt ttactgaaaa
     1021 taaagtgccg ctacatcctg gtcctgttga aaataatttc aaattgttca gaaaaactga
     1081 ctggttggca ggattttgca aaaatcttat ctgaacttag catttaatgc aataatcctg
     1141 ttatcatgaa aaaaagaaaa agttcagttt taattttctt cattgtactg gttttgttga
     1201 attttctgaa tgtaataccc ggaaattcac aaaaagccag tgatcacgca gagattggcc
     1261 gttatggcac ttcctcgatc agttaccagg aactggccaa tttcaatgaa tataaactcg
     1321 ctctcggacc ggtaaggaat gattcggtaa gtgaaatcct gaaaattcag ggtaaggtga
     1381 tgatgactct ctaccaggga cctgccgatg cttcaagtta tgaaatttat catgcctatc
     1441 gcgatctgct gatatcaaag cagtatgata tactttactc ctgttccggg accgaatgcg
     1501 gaaccgcatt tctttcaccg ctttataaac tttcggcatt tgcctacgac cacggatata
     1561 ataattccgc tcccattaca caggggtcgc aggatttctc ttacctgatc actgcaagga
     1621 aaagcaccgg ggagaaaact acttatgtta gtttaattgt ttcgcacgga tggtttaaat
     1681 accctgtcta taaacttgat gtcattgaaa tgcaaaagcc tggcggaaaa atatcaagta
     1741 ttcaggcagc taaagaggat gtgccgtcag acagaactgt tccggacaga tcaggaacct
     1801 tgcatgaaaa agtagcgggc cggcctgcgg aagccgtatc gccggctatg actcctgacc
     1861 gtagtccgga gagggatgaa gatgcgggag ccgtccgttt tggctttcac atgtcagccg
     1921 attcctattt tggcctgctt cttcgtgcca gacattttga gttcgtcatt aagggttatg
     1981 ccgatctttt cgacggattc ccggaaggtt acagaccgga cgatttcctg atgatcgggg
     2041 gacatatatc ttatcttttc cggccctggg aaaaggcaga cattggtgtt ggtcttgatc
     2101 tgcgacaggg tatctccata acaggcaata taaaatatgt tcagtatata gatgccggtc
     2161 cggtgctaag ttttaactac agccccggaa agcattttgt catttccgga ctattttacc
     2221 cggtctggct gaatacaagg gaaacggata tggacgacag cttcagtctg gcggttcaga
     2281 tacccagagc ttcagtagcg ctgggattct tgttctgagt tattgtttgc ctttcaaagg
     2341 ttgatcttcc cgcggatccc gcaggaacag gtaaaaaaat tgaatattta aagcaggata
     2401 tgctataaat gtcttaattt atccggctat attgcattaa tcagatcaaa agccaggata
     2461 atgaaaaatc taattttaaa tttcagtgtt ttgaccatcc tgatttgttt ttacggatgt
     2521 tcgtcagatg ttcaatcgaa taaaaagaaa atcagcccgc cagggaacga ttattatgtt
     2581 gtagcctata tctggccttc atgtcatgat gacccgctcg gaagaaagat gctctggccg
     2641 gaagggacag gtgaatggga agtgattaaa aagggcaatc cgcgttatga ggggcattat
     2701 cagcctcgtc tgccgctctg ggggcatgag ccggataacg atccaaaagt ggttgaaaga
     2761 tggatagatg ttgcaacaga ccatggcatc aatgttttcg tttacgactg gtactggtat
     2821 gacggaggac catttctgga aagtgctctc aatgatggct tcctgggtgc agtgaacaac
     2881 gaaaaaatgc agttctatat catgtgggcc aatcatgatg taaagtataa ctactggaat
     2941 taccatcttc ataaagacaa tacggatatt ttatgggacg ccaaagtaga ccgggaaaat
     3001 ttccgcatca ttgtcgacag ggtcatcaac cagtatttca agaggccgaa ttatttcaag
     3061 atcaatggtg agcctgtttt ttccatattc agtataggca aactggtgga aagctttgac
     3121 aacagtctgg aagaagcccg gaaaggtctg gaatatttcc gtgatgaagt caggaaggcc
     3181 gggtttccgg gcctgcatat tcagtggaat cagggcggtg gttctattat gtcggaaaaa
     3241 gctgccggaa actttgtcaa cagtgtcaga accatgggtt tcaacagtgt tgccatgtac
     3301 aacatgggcg gacttgatga ggattatctt gtttatggag ccaattccct gaagatacgt
     3361 gagcaaatgg actcaattct ggatgtgccg gtattcccct gtgtgtccgt cggatgggac
     3421 gacacaccgc gtttccccga taaggggatt cacgacgtgg tacattatca caatacgccc
     3481 caaagctttg cggctctgct ggccaaggca aaaaaatatg ccgacagtca tcctgagcag
     3541 cccagactga ttaccatcaa tgcctggaac gagtgggtgg agggcagtta tctcctgcct
     3601 gacatgcttc acggcttcgg atacctggaa gcagtaaggg aggtgatcct tgaaggtaaa
     3661 tatgacaaat attaaaaatg gcatcaggtt ctggactgat taaatatccc gggaaatgga
     3721 aaagaaggaa acaggcagtt ccagacggga agttctgaaa aagctggctg ccacatcagt
     3781 tgtaatatta acaggaggaa aaatgaaagc agaaacatta aaagaccggc aggccccgga
     3841 agcgggactg aaaggcaata tcaaccattc agcatgccgc tggtgctatt cttcgatacc
     3901 cctggaagag ttttgcaggg gggcaaagga aattggcctg aaggcaattg atcttaccgg
     3961 tcctgacgaa tggcctgttc taaaaaaata cggacttgac tcttcaatgt gcaatggtgc
     4021 ggagattgat atttcaaggg gctggaatga cagccggtat cattccacac ttgtgaaaaa
     4081 ctattctgaa atgattcctc ttgttgcaaa ggcagggtat aaaaatctta tttgtctgag
     4141 cggaaaccgg gacggaaaag atgatgagac cggtctgagg aactgtgtgg aaggactgaa
     4201 gcaggtattg ggcctggccg aaaaacatgg tgtgatgctt gttatggaac ttctcaacag
     4261 cagggtagat catccggatt atatatgtga ccggacgtgg tggggagttg agcttgttaa
     4321 aagagccggc tcggagaacc tgaaactgct ttacgatata taccatatgc agataatgga
     4381 gggagacatt atccggacaa tccgggataa ccatcagcat attgcccatt accatactgg
     4441 cggtgttcca gggcggaatg agatcgatga cagccaggag ttgtattacc cggcaataat
     4501 gcgggcaatt gtggaaacgg gctttaaagg atatgttgca cacgagtttg tgccttcccg
     4561 ggatgacaag ctggaatccc tcaggcaggc agttgccata tgtgatgttt agagacaggc
     4621 agatgttacc aaaagacaag ccggtataac aatattcagt taaagtaagt taaattcatt
     4681 aaatataatt gacaggatga gccacgtttg aattttttaa tatttttgta tcgccgggat
     4741 taaaaagata aaacccattt tttaaaaaag aatatacttt ttattgaagg acaggtgagg
     4801 agaggataat ttttttttaa cttgccggta acacgaataa gagaattgtt aataccatta
     4861 tcccgtttta gttttgggat tttattttga tagtatagta
//
LOCUS       DAOSPX010000250         4867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15830_length_4867_cov_3.884871, whole genome shotgun sequence.
ACCESSION   DAOSPX010000250 DAOSPX010000000
VERSION     DAOSPX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15830_length_4867_cov_3.884871"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PLZ75_10930"
     CDS             <1..1073
                     /locus_tag="PLZ75_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS72946.1"
                     /translation="FHAWLSPFLMLNSVFALKAIIWLNMAAVAVLAAVMIRKRWFCKY
                     LCPAGWCFDKISSLSPFAGRTYNKLPHAGKWLAVISLVSALFGFPLFVIFDPLSLFNG
                     FFLNLAGKPEPVVFISLLPFPLLLLMHFFLPGIWCKKLCPLGGLQTGLWDIKSHLSGL
                     NLKKAPVSETELPGRRYFVMSGAGLLAGLIMPGILRSSPVKVIRPPGTRDSLSFNTLC
                     SRCCNCIRACPTRIIQPLTVCDQPLAWMTPVIRYENGYCLETCNICSRVCPTGAIAFF
                     PVDEKKHVFIGKAEISSENCYLLNNRECIKCREVCKYEAISYMPGTGTNTGVPVADLS
                     KCVGCGACEIVCPADCIVVRPL"
     gene            1437..2564
                     /locus_tag="PLZ75_10935"
     CDS             1437..2564
                     /locus_tag="PLZ75_10935"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOS72947.1"
                     /translation="MKRILLLTLVFAGAAAFLPSCNQSKKSEIVRKEIYGTYQGKEVY
                     MFTLTNKPGNIVKITNFGGKINWIEVPDRNGKKENITFGYDTFEGTINGDMSYGSLVG
                     RYANRIANARFSLDGVEYELPVNNGPNCLHGGPKGWHSVVWDAEVVSGSKFPAIRLTY
                     VSPDMEMGFPGTVTAEVVYTWTDNNEIVMDYKCTTDKKTVVNVTNHAYFNLLGPGKGD
                     ILGHVLTLKASAFTPVDSVMIPTGELRPVAGTPFDFTSPHTIGERIGEDYDQLILGRG
                     YDHNFVLDNKEPVDVEVYEPVSGRVLEVITDQPGMQLYTGNFLDGTNIGHGGVAFTHR
                     SGFCLESGHFPDSPNHPDFPTTVLNPGETFKSQTIYRFSAR"
     gene            2758..4365
                     /locus_tag="PLZ75_10940"
     CDS             2758..4365
                     /locus_tag="PLZ75_10940"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HOS72948.1"
                     /translation="MTAGKKVEYGLKSITADDMKTHISILASDEFLGRAPSTEGEEKT
                     INYLAEEFRRIGLEPANNGSFFQEVSLVKITADPEMKVDISGGRNRINLKYREDIIGG
                     SPQQKEFIRLDNSGIIFVGYGINSPENNWNDYEGLDVKGKTVLMLINDPGYATSDETL
                     FEGKTMTYFGRWTYKYEEAARQGASAVIIIHETGAAAYPWEVVQNSWGGSQFSLRNDP
                     LASSALQFCGWITNESGRKIFQAAGLDFDGLTRAASGRDFRAVEMNMGLSVNFRNEVE
                     YSVSDNVAALWPGGRKADEYIIYCAHWDHFGVSNTFRGDSILNGAVDNATGTAALLEI
                     AEAFANLPARQNRSILFLSVTCEEQGLLGSDFYANNPLFPPGKTAAVINMDALNIFGR
                     TRDMIISGYGYNQLDDYAIAVLEKYGRYASPDPTPEKGGYFRSDHFSFAKVGIPSINL
                     SAGVDNVEQGKAWGLARSEEWIRENYHEPSDNYEPDKWDFDGMIEDVKVYFETGYNLS
                     MTDKFPEWNPGVPFKALRDEMMENQKP"
     gene            4380..>4867
                     /locus_tag="PLZ75_10945"
     CDS             4380..>4867
                     /locus_tag="PLZ75_10945"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS72949.1"
                     /translation="MKNSILAVIIITVLTSCSGKEKGWRQLFNGRDLTGWEQIGPGKF
                     EVEDGLLKTVGGMGMLLYPSEKFGDVTIRVVYKVEDNDCNSGVFIRIPEIPSDEWYAV
                     NRGYEVQIDHGTRHVGGEYHCTGVLYSLTKAMAYPQKEPGEWNTMEITLDGPRTIVVV
                     ND"
BASE COUNT         1344 a          970 c         1212 g         1341 t
ORIGIN      
        1 gattccatgc atggctcagt ccctttctga tgcttaattc cgtttttgca ttaaaagcta
       61 ttatctggct caatatggca gccgttgccg tattggcagc tgtcatgata cggaaacgct
      121 ggttctgtaa atatctttgc cctgccgggt ggtgttttga taagatatct tcactcagcc
      181 catttgccgg aagaacatac aacaaactgc ctcatgcagg caagtggctg gcagttattt
      241 cacttgtgtc tgctttgttc ggctttcccc tgtttgtgat ttttgatcca ctttccctgt
      301 ttaacggatt ttttcttaat ctggccggga aaccggaacc tgtggttttt atttcccttc
      361 ttccgtttcc gctgttattg ctgatgcatt tctttctgcc cggaatctgg tgcaaaaaac
      421 tttgtcctct cggaggactg caaacaggcc tgtgggatat aaaaagccat ctgagcggat
      481 tgaatttgaa aaaagccccg gtttcagaga ctgaacttcc cggcaggcgc tattttgtca
      541 tgtcaggagc cggtttgctg gccggcctga ttatgcccgg catacttcgt tcttctcctg
      601 tgaaagttat ccgtcctccg ggaacaaggg acagtctgtc gttcaatact ctttgttccc
      661 gttgctgtaa ttgtataagg gcttgtccga ccaggatcat tcagccgctt accgtttgtg
      721 accagccact tgcctggatg actccggtaa tcagatatga gaacggatat tgccttgaaa
      781 catgcaatat ctgcagccgg gtctgtccga ccggggcaat tgcatttttt cccgttgatg
      841 aaaaaaagca tgttttcatc ggaaaggctg agatcagcag tgaaaactgc tatcttctga
      901 ataacaggga atgtataaaa tgcagggaag tatgtaaata tgaagccatc agttatatgc
      961 ccgggaccgg aaccaatacc ggtgttcccg tagcagacct gtcaaaatgt gtcggatgcg
     1021 gggcatgtga aatagtatgc cctgctgatt gtattgttgt caggcccctg tagcgaaagc
     1081 gcaattcgta taaacaatgg aataaatacc ggttttgccg gtttgatttt tctgcttgtg
     1141 agatacatgc ttagatgtgg aataaaatat cgcggatgct gtaatattcc ggataagtga
     1201 cggatatctc cttattttgt accagggttt ttaaggtatt ttgtttttaa gtatttgcag
     1261 cagatttttc tgattgatta accgggaaat atcttaactt tattaactca ttgttttgtt
     1321 ctttatgcgc tgttctgaac ccattgcgaa tgaggctttt caggagccgg cataagacgg
     1381 tactgagtga aataatgatt tacgggaatt tatggattaa acagaataaa cagaaaatga
     1441 aaagaatttt attattaaca ttagtttttg caggagctgc tgcatttctg ccatcctgca
     1501 atcaatcaaa aaagagcgaa atagtcagga aagagattta cggtacttat caggggaaag
     1561 aggtttacat gtttacactc acaaacaagc ccggaaatat tgtcaaaatt acaaacttcg
     1621 ggggaaagat caactggatt gaggttcccg acagaaacgg aaagaaggag aatataacat
     1681 ttggttatga tacctttgag ggtacaatca atggtgatat gtcatacggc tcccttgtcg
     1741 gcagatatgc caacaggata gcaaatgccc ggttctccct tgacggtgtt gaatatgaac
     1801 ttcctgtaaa taatggtcct aactgcctgc atggcggtcc gaaaggctgg catagtgttg
     1861 tatgggatgc tgaggtggtt agcggaagta aatttccggc tatcagactg acatatgtca
     1921 gtcccgatat ggaaatgggc ttcccgggaa cggttactgc cgaagttgtc tatacatgga
     1981 ctgataataa cgagatagtg atggattata aatgcacgac agacaagaaa actgttgtaa
     2041 atgtaaccaa tcatgcttat ttcaatttgc tgggtccggg aaagggtgac atactggggc
     2101 atgtcctgac cctgaaagca tcagcattca caccggtcga ttcggtgatg atacccaccg
     2161 gtgaattaag gcctgttgcc ggcactccgt tcgattttac aagtccgcat acgattggcg
     2221 aaaggatagg ggaggattat gaccagctca tactgggccg cggttatgat cataatttcg
     2281 tgctggacaa caaggagccg gttgatgttg aggtatatga gccggtaagc ggaagagtcc
     2341 ttgaagtaat taccgatcag cccgggatgc agctttatac cggtaatttc cttgacggaa
     2401 caaatattgg tcatggcgga gtggctttta cccacagatc aggattttgc ctggaatcgg
     2461 gccattttcc tgacagtccc aaccatccgg attttccgac aacagtgctt aatccgggag
     2521 aaacattcaa atcacagacg atttacaggt tctcagccag gtaaatcatc aggtccctgc
     2581 ttgttatccg gaggcttcaa cagaaagatg gtcattgact tcctcaatgc atcctgcagg
     2641 tcttaaacag ataataagct tgatataagc atcctgtatt tttaaaatca ttatcatcat
     2701 taagaaatga ggaatatttt tgttatcttt ttttctctga ctttcctggc aggttgcatg
     2761 acggcgggga aaaaagttga atacggactg aaaagtatca cagctgatga catgaagacc
     2821 catatttcga tacttgcttc cgatgagttc cttggacggg ctccttctac cgaaggggaa
     2881 gagaaaacca ttaattatct cgctgaggag ttcaggagga tcgggcttga accggccaat
     2941 aatggcagtt ttttccagga agtgtcgctg gtaaaaataa ctgcagatcc ggaaatgaaa
     3001 gttgatatca gcggaggaag aaacaggata aacctgaaat atcgcgaaga tatcatagga
     3061 ggaagccctc agcaaaagga gtttatcagg cttgataatt ccggaatcat atttgtcggt
     3121 tacgggatta attctccgga aaacaactgg aatgattatg aaggtcttga tgtgaagggc
     3181 aaaaccgtgt tgatgcttat aaatgatcct ggttatgcca cctccgacga aactctcttt
     3241 gaagggaaga ccatgaccta tttcggacgc tggacatata agtatgaaga agcagcacgg
     3301 cagggagcct cggcagtcat catcattcat gaaacaggag cggctgcata tccctgggaa
     3361 gttgttcaga acagctgggg cggttcgcag ttttctctca ggaacgatcc tctggcttca
     3421 tctgctcttc agttctgtgg ctggataacc aatgaatcag gcaggaaaat ttttcaggct
     3481 gcaggactgg attttgatgg gctgaccagg gctgcttccg gaagggattt cagggctgtt
     3541 gagatgaaca tgggattatc cgtcaatttc aggaatgaag tggaatattc tgtatccgac
     3601 aatgttgctg ccctgtggcc gggcggcagg aaagcggatg aatacataat atactgtgca
     3661 cactgggatc atttcggtgt cagcaatact ttcaggggtg attcaatact gaacggtgcc
     3721 gttgataatg ccactggtac agcggcctta ctcgagattg cggaagcatt tgcaaatctt
     3781 ccggcaagac agaaccgttc aattctgttt ctttcagtaa catgcgagga acaaggactg
     3841 ctgggcagtg atttttatgc aaacaatcca ttgtttcctc cgggaaaaac agccgctgtg
     3901 atcaacatgg atgccctgaa catttttgga aggacaaggg atatgataat ctccggttac
     3961 ggatacaatc agcttgatga ttatgcgatt gcagttcttg aaaaatacgg gcgctatgca
     4021 agccctgatc ccacacccga aaaaggaggt tatttccgtt ctgaccattt cagttttgcg
     4081 aaggttggaa taccttccat taatctgtcg gcaggggtcg acaatgttga acaaggcaag
     4141 gcatggggcc tggcccggag cgaggaatgg atacgggaaa actaccacga gccttctgac
     4201 aactatgaac cggacaaatg ggattttgac ggtatgattg aagatgtgaa agtttatttc
     4261 gaaaccggct ataatctcag catgacagac aagtttcccg aatggaatcc aggtgtccct
     4321 tttaaagcat tgagggacga aatgatggag aatcagaaac cttaaaaaac aatgcacata
     4381 tgaaaaattc tattcttgcc gtaatcatta tcacggtttt aacctcatgt tccggtaagg
     4441 aaaaaggctg gcgccagctc tttaacggga gggatctgac cggctgggaa cagataggcc
     4501 ccggaaagtt tgaagtggaa gacggtctgc ttaaaacagt aggggggatg ggtatgcttc
     4561 tttacccttc cgagaaattc ggggatgtga caatacgcgt tgtttacaaa gtggaagaca
     4621 atgactgtaa ttctggtgtt tttataagga taccggaaat tccttcagat gaatggtatg
     4681 cggtgaacag gggatatgaa gtgcagatag atcatggaac caggcatgtt ggaggagaat
     4741 accattgtac gggagtgcta tactctctta ccaaggccat ggcttacccc cagaaggagc
     4801 cgggtgaatg gaacacgatg gaaataaccc ttgacggacc aagaaccata gtagttgtaa
     4861 atgatga
//
LOCUS       DAOSPX010000251         4860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15862_length_4860_cov_4.333403, whole genome shotgun sequence.
ACCESSION   DAOSPX010000251 DAOSPX010000000
VERSION     DAOSPX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15862_length_4860_cov_4.333403"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PLZ75_10950"
     CDS             <1..1064
                     /locus_tag="PLZ75_10950"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOS72950.1"
                     /translation="SAMKKKDIMLYFPYHPFSYLIDLLREASIDPYVTSIHITLYRLA
                     RNSNVINALLNAVRNGKKVTTVVELQARFDEEANILWGKKLMDEGVKVIYGIPGLKVH
                     AKLLLITRIRDDVVKRYAAVGTGNFNEDTARIYCDHLLLTSNLKITNEVFKAFNFLNI
                     NYKKDNYYHLVLSPFSLRNKIILLIENEIKNARAGRKAYIHLKLNNLTDNEIINYLYE
                     ASAAGVSIKLIVRGMISLVPGMKDLSENVRARGIVDRFLEHSRFMIFHNNGKEECYIS
                     SADLMPRNIDRRFEVTCPVFDKNLVNELKKLFQIQWNDNVKARIIDHGFTNKFVKTGK
                     KAFRSQLEIYNYLKEIHSK"
     gene            1080..1604
                     /locus_tag="PLZ75_10955"
     CDS             1080..1604
                     /locus_tag="PLZ75_10955"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOS72951.1"
                     /translation="MNLIILIAGIFVIAFFSWTYSVRDRRYHGIPRFFSFLSIFVLFL
                     LNYKIWFRNVFSWHQILSWILLIASAYPGIAGFLTLKNKGRSEDSFENTTMLVRSGVY
                     KYVRHPLYCSLLLIGTGIMFTLPGIPQIILGLTNALSLYLTAIIEEKEMISRFGDQYS
                     EYMKETKMFIPFLF"
     gene            1679..2698
                     /locus_tag="PLZ75_10960"
     CDS             1679..2698
                     /locus_tag="PLZ75_10960"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOS72952.1"
                     /translation="MRIGILTAGGDCPGINAAIRGVGKTAIFKYGMKLYGISDGFSGM
                     ITKEYTELSEKDLSGILTLGGTILGTSREKPFKPADKNNKELKKPALIKEHYEQMGLD
                     CLVCIGGNGTQKTASLLAREGLNVVGIPKTIDNDVWGTDITFGFDSAVHIATEAIDRL
                     HSTANSHKRIMIIEVMGHHAGWIALYSGIAGGGDVILIPEIPYQENRIAEYLLQRAEN
                     NKAYSIVVMAEGVKNPEKGISAAQYLSRRITELTGLETRETVLGYVQRGGNPSHYDRV
                     LATRYGTEAADMIARRDFGKMVALRKNDIVPIPLDDVAGKLKLIEPGNSLVQLTKNMG
                     TSFGE"
     gene            2711..3826
                     /locus_tag="PLZ75_10965"
     CDS             2711..3826
                     /locus_tag="PLZ75_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72953.1"
                     /translation="MRKRTGIFLSAISGILISIMPSSYAQMLHDSAAVRKITTGVDYL
                     YNMEFARASSMFSEVEKLYPGHPVNYLLKGISLYWKDYPMVPTSEYWQAFENNLRTSI
                     DLCSENQYSARYESESLLTNVCSRGLLLLCYNENNLSFKVITLATNTYRYIMKSFDHV
                     TAFADFYYPTGLYNYYREAYPQMNPVYKPLASLFPPGDMKRGLTELSRAAELSIFLKA
                     ESYSVLSFIYAGFENDFRTALLYSGMLTKKYPSNIYYKALHMRNLLVMKEYGLVEEMI
                     KTTVNNKKNAYFDAQLMIIKGIIQEKKYKNFALARQLYEGGISAIAFTGDYGNEYCGY
                     AYLGLSRICDHEGDKAGKKAFRKKGISLLHFKKITFD"
     gene            complement(3881..4471)
                     /locus_tag="PLZ75_10970"
     CDS             complement(3881..4471)
                     /locus_tag="PLZ75_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOS72954.1"
                     /translation="MKNRVLKTGITILFPCVMLILMPFTIFSQTSNFYSFKIKTLEGN
                     DFNLDSLKGKKVMVVNTASKCGYTPQYKDLQELYEQYSRKLVIIGFPANNFGNQEPGT
                     ALEIRQFCTDNYNVTFPMMEKISVRGEDIHPLYKWLTSKEENGVMDSEVKWNFQKYLI
                     DEEGKLVDVLYSKEKPGSEKVISWLNSPGEKKSVSP"
     gene            complement(4640..>4860)
                     /locus_tag="PLZ75_10975"
     CDS             complement(4640..>4860)
                     /locus_tag="PLZ75_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOS72955.1"
                     /translation="RVMHIHGINEKQARERIDKTDKQRESYYNYFSSKLWGAAGSYHL
                     CINTSVLGIEETTDFIKCFAEKRFRLTH"
BASE COUNT         1472 a          997 c         1035 g         1356 t
ORIGIN      
        1 tttcagcaat gaaaaaaaag gatatcatgc tgtattttcc atatcacccg ttcagctacc
       61 tgattgatct gctgagggaa gcatccattg atccatatgt tacctcaata catataaccc
      121 tctaccggct tgccaggaac tccaatgtta tcaatgcttt gctgaatgca gtgagaaatg
      181 gcaagaaagt gaccacagtc gttgagcttc aggcacgctt tgatgaggaa gcaaatatcc
      241 tctgggggaa aaaactaatg gatgaaggtg ttaaagtgat ctatggcata cccgggctta
      301 aagtccatgc aaaactgctg ctgatcacaa gaatcaggga tgatgtcgtg aaacgttatg
      361 ccgcggtagg cacaggtaat tttaatgagg atacagccag gatttactgt gaccatctct
      421 tactcacttc aaatctcaaa atcacaaatg aagtgttcaa ggcattcaac ttcctgaaca
      481 tcaactataa gaaagataat tactatcatc tggtcctgtc gcctttctca ctccgcaata
      541 aaataatcct gctgattgaa aatgagataa aaaatgcgag ggccggcagg aaagcctata
      601 tccatctcaa attgaacaat cttactgata atgagataat taattatcta tatgaggcaa
      661 gtgccgcagg agtaagcatt aaactgattg tacggggcat gatatcactg gtgccgggaa
      721 tgaaagattt aagtgaaaat gtcagggcca ggggtattgt tgacagattt ctggagcaca
      781 gcagatttat gatattccat aataacggta aggaagaatg ctatataagc tcagctgatc
      841 tgatgccgcg gaatattgac cgccgctttg aagtgacctg tccggttttt gataaaaacc
      901 tggtaaatga actcaagaag cttttccaga tccagtggaa tgataatgtg aaagccagaa
      961 tcattgacca cggatttaca aataaatttg taaagacagg aaagaaagcc ttccggtccc
     1021 agctcgagat atacaattat cttaaggaaa tccattcaaa atagcgggag aaggataaaa
     1081 tgaacctgat aatcctcatt gccggaatct ttgtgatagc atttttttcc tggacttatt
     1141 cggtaaggga caggagatac cacggtattc ccaggttctt ttctttcctg agtatttttg
     1201 ttcttttcct cttaaattat aaaatatggt tccgcaatgt cttcagctgg catcagatat
     1261 tatcatggat tttgctgatc gccagtgctt accccggaat tgcagggttt ctgactctta
     1321 aaaataaagg caggtcggag gatagttttg aaaacaccac aatgctggtc aggtctggtg
     1381 tttacaagta tgtgaggcat cccctttatt gttcgctgct tctgatcgga acagggataa
     1441 tgttcacgct tcccgggatc cctcagatca ttctcgggct tacaaatgcc ctttcacttt
     1501 atttaacggc tataattgag gaaaaagaaa tgatatccag attcggggat cagtattcag
     1561 aatatatgaa agaaacaaaa atgttcattc cttttttatt ctgataaaac ctgtttgaga
     1621 gtaaaaaatt agttttgtcg gttaatttat tatcaatatg agcagaaaaa gcaatggcat
     1681 gaggatcgga atcctgactg ccggaggtga ttgtcccggc atcaatgccg caatacgcgg
     1741 agtcggaaaa accgcaattt tcaaatacgg tatgaaacta tacggtatca gcgacggttt
     1801 ttccggaatg ataaccaagg aatatactga actgtcagaa aaggacctgt cgggaatact
     1861 gaccctggga ggaaccattc ttggcacttc cagagaaaaa ccctttaagc ctgccgataa
     1921 aaacaacaag gaattaaaaa aaccggctct gataaaggaa cattatgaac agatgggtct
     1981 tgattgcctg gtctgcattg gcgggaacgg aacacagaaa acagccagtc tccttgcacg
     2041 ggaaggtctg aacgtcgtgg gcattcccaa aaccattgac aatgatgtat ggggcacgga
     2101 tatcactttc ggattcgatt ctgctgtcca catagctact gaagccatcg acaggctcca
     2161 ctcgactgcc aattcacaca aaaggataat gatcattgaa gtcatgggcc atcatgccgg
     2221 ctggatagct ctttattcag gaattgccgg cggtggcgat gtgatcctta taccggaaat
     2281 tccataccag gaaaacagaa ttgccgaata cctgcttcag agagcagaaa acaacaaagc
     2341 gtattcaata gtggttatgg cagaaggagt caaaaatccc gaaaaaggca tttccgctgc
     2401 acagtacctt agcagaagga tcactgaact taccggactt gaaacgaggg aaactgttct
     2461 gggctatgtt cagcgcggcg gcaacccttc ccactatgac cgcgtgcttg ccaccaggta
     2521 tggaacggaa gcggccgaca tgatagcacg cagggatttt ggaaaaatgg tggcgctgag
     2581 aaaaaatgat attgtgccta ttccccttga tgacgttgca ggaaaactga aactgattga
     2641 acccggcaat tcccttgttc agctgactaa aaatatgggg acaagttttg gagaataaac
     2701 atattcctca atgagaaaac ggacaggaat tttcctttca gcaatttcgg gcatcctgat
     2761 cagtataatg ccatcatcat atgcccagat gcttcatgac agcgcagctg tcaggaaaat
     2821 aacaacagga gtcgactatt tgtacaacat ggaatttgcc agggcctcat ccatgttttc
     2881 ggaggtggaa aaactgtatc ccggacatcc ggttaattac ctgttaaaag ggatatcact
     2941 atactggaag gattatccta tggtgccgac atcagaatac tggcaggctt ttgaaaacaa
     3001 cctcagaacc agtattgacc tctgttctga aaatcagtat tcagccaggt atgagtcaga
     3061 gagtcttctt acaaatgtgt gttcaagggg attactgctt ctttgctaca atgaaaacaa
     3121 ccttagtttc aaggtaataa ctcttgcaac aaatacttac aggtatatca tgaaatcctt
     3181 tgaccacgtc accgcttttg ctgatttcta ttatcctacc ggactgtaca actattacag
     3241 ggaggcatat ccgcagatga atcctgtgta caagcccctg gcctccctgt ttcctccggg
     3301 tgatatgaaa aggggactga ccgaactctc ccgggcagct gaattatcaa tattcctgaa
     3361 agcggaatcc tattcagtac tgtcattcat ttatgccgga tttgagaatg atttccggac
     3421 agcccttctc tacagcggga tgctgacaaa aaaatatcct tccaacattt actacaaagc
     3481 ccttcatatg agaaatctgc tggtaatgaa ggaatatgga ctcgtggaag agatgattaa
     3541 aacaacagtg aataataaaa agaatgcata ttttgatgcc cagttaatga ttatcaaagg
     3601 cataatacag gagaaaaaat ataaaaactt tgcgcttgcc aggcaattat atgagggcgg
     3661 aatctcagca atcgcattca ccggtgatta cggcaatgaa tactgcggtt atgcttatct
     3721 gggcctgagc aggatatgtg accacgaagg agacaaggcg gggaaaaagg ccttccggaa
     3781 gaagggaatc agcctccttc acttcaagaa aattacattt gactgaaatg tttcccggat
     3841 ggatgtactg ctatttttcc ccgggaatga atatgaaaaa tcagggactg accgattttt
     3901 tctcaccggg cgaattcagc caggatatca ctttctcgga accgggtttt tcctttgaat
     3961 agagaacatc cacgagcttt ccttcctcat ctatcaggta tttctgaaag ttccatttta
     4021 cttccgaatc catgaccccg ttctcttcct ttgatgtaag ccatttatac aagggatgaa
     4081 tatcctcacc cctgaccgat atcttttcca tcatgggaaa ggtaacatta tagttatcgg
     4141 tacagaattg cctgatttca agagcagtgc ccggttcctg gtttccgaaa ttatttgccg
     4201 gaaatccgat gatcaccagc ttccggctgt attgttcata cagctcctgc aaatctttgt
     4261 attgcggcgt ataaccacat ttggatgctg tatttacaac catcaccttt ttgcccttaa
     4321 gggaatccag gttaaaatca ttcccctcaa gtgttttaat cttgaaacta taaaaattac
     4381 ttgtctggga aaatattgtg aatggcataa gtatcaacat cacacatgga aagagtattg
     4441 taattcctgt ttttaaaact cggtttttca tgagacacac tggttaataa ttaaatttct
     4501 gtacagttag tattctgttc ctgcctggca tgcttgtcag gttaaaattc agagagctta
     4561 catcatggtt tcaggtatat aagggtaatt tcaaggctca agttacgaaa attattaata
     4621 acaattcaag actacctttt cagtgcgtga gtctgaatct tttttctgca aaacacttta
     4681 tgaaatctgt tgtttcttca attccaagaa ctgaagtatt gatgcaaaga tggtatgatc
     4741 cggcagctcc ccataattta ctgctgaaat agttgtagta tgattcgcgt tgtttatcgg
     4801 ttttatctat acgttcacgt gcctgctttt cattgatccc gtggatatgc atcactcttc
//
LOCUS       DAOSPX010000252         4852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15888_length_4852_cov_4.096936, whole genome shotgun sequence.
ACCESSION   DAOSPX010000252 DAOSPX010000000
VERSION     DAOSPX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15888_length_4852_cov_4.096936"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLZ75_10980"
     CDS             complement(<1..409)
                     /locus_tag="PLZ75_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72956.1"
                     /translation="MMNSLRLIIKELLYRRGSTLVILLTVIIASTVSVAIYTLSKASE
                     NETRKIMREQGLNLYIFPKGTNLIDFFSVNDTPVFPEDYVEVLAGSRTFDAVRHLTGI
                     LQVKYNDWKDPNGSSHQILLVGYKDEAMQRFLPK"
     gene            complement(462..683)
                     /locus_tag="PLZ75_10985"
     CDS             complement(462..683)
                     /locus_tag="PLZ75_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72957.1"
                     /translation="MKQDTLQYRSIDDRPLPKWLTDQRDGLNRYQPEKLEMKEDNSLT
                     LSFLLTGGIIILVIAVLTVYVLKKRPNRY"
     gene            complement(683..1471)
                     /locus_tag="PLZ75_10990"
     CDS             complement(683..1471)
                     /locus_tag="PLZ75_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72958.1"
                     /translation="MKPVYLRILVLSGILSMACTTKVSEWVLLNSEANNYLLVYYHKD
                     PVTEPVKKQNEQIGREINSANIQFRSILRNDIEKPYYALIYGNRLFSTYNNHAELKNL
                     SVSPLREKIASELMSGMLCVMLYLKTGNPEKDAPASEIIKRTVAASPFREVVSVRELD
                     RSSTEEKHFVSMLLNVENDLKEINEPMLFGIFARFKALEPLLARGISTENINLMIDFL
                     TADCSCLIKDNLPGTDILFTNNWENPLPALVNRILDENPALLHH"
     assembly_gap    1497..1506
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1507..>4852)
                     /locus_tag="PLZ75_10995"
     CDS             complement(<1507..>4852)
                     /locus_tag="PLZ75_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS72959.1"
                     /translation="HFFTDGPVRLPLAYSKGKIFFTSDDGYLYCLSAESGQLLWKKML
                     APASNKLLGNKRFISMWPARGGIVIQDDVIYTASSIFPLMGTFIYAINAADGEIIWKN
                     EGTGSNYILQPHNSPAFADIAPQGCFTISGDRLLVAGGRSVPAGFDKATGEKLFYQLA
                     PSGKTGGAFTCANDRVFFNHHRERLTGMYDSENGKKLIPVAGEYPVIEGDKIYFSGKD
                     ITCVFLDESKKLDTLWTIDVPAGTDLIKAGNCLYAADSSGIYAVRISGSGKPELVWKY
                     SPEKVIDRLVASNGKLIAVANDGTIMAFGDKAADRVLVHEKAVAGPRKVSGKIQRLIS
                     SAGISEGYALVFGSDMGLLSGLMKETSLRTVVYEKDRQKVEALREHFNKLGIHADRLM
                     FLEFEKPNPVLPKYFSSLTVINENFGFDNEFLEMIYESTRPYGGKILIRTGRTEREEL
                     ERTFLSLNLYGAVMKSDRLYSMITREGSLEGSSAWTHNYGDIANTVKSDDKRVRAPLG
                     ILWFGGNSNMDVLPRHGHGPGEQVVDGRLIIESLNGISARDVYTGRVLWKRDFRSLKD
                     DFWLVYYDESYDEENPLDPKYNQVHLPGSNARGTNFIATREYVYVIEGDTCHFVDIKT
                     GDLIKSFTTGDEETKELGYIGVYEDLLILGKNFSEYTGLENDSMKIKNPKFTDYNLTA
                     SNELIVVNRFTGERIWSMESNHGFIHNSVIAGDDILFCLDKLPQYVETKLKRRGESPP
                     EGSRLLYVDIRSGRILNEVTEDVFGTWLGYSSEHRLLLQATRPSRDMLHGEEGNRMIT
                     YNVLTGEKIWDKALKYANPPIIHNDKIYTNGEGFHLLTGEILFEKDPLTQEDLKWNFK
                     REYGCGIVAASEHLLTFRSASAGFVNLDVFEGTGSLGGWKAGCSTNLIAADGVLNSPD
                     YTRTCQCPYQNQTSLALVYMPWMTYWTNSNYKWDGKKIRQFGLNLNAPGDRTSENNTL
                     WMEYPFVSGSDPGIPVRMDTLNYYRIRKEPISVVSRGTPWVSASALCGVRSFELTIAD
                     DEDVTETWYTVKLYFSELENKKPGERVLNISLQGEEVLRNFDIVSEAGKTDKEVIKSF
                     TGIRAGRTLRLDLIPLEGNTIISGVEM"
BASE COUNT         1218 a         1202 c         1000 g         1422 t
ORIGIN      
        1 gtttcggaag gaaccgctgc attgcctcat ccttgtatcc gacaagcagg atctgatggc
       61 ttgaaccgtt cggatccttc cagtcattat acttcacctg gagaattccg gtaaggtggc
      121 ggaccgcatc gaaagttctt gaaccggcca gaacctccac ataatcctcg ggaaagaccg
      181 gagtatcgtt tactgaaaaa aaatcaatca ggttggttcc cttcggaaat atgtaaaggt
      241 tcagaccctg ttccctcatt attttacggg tttcattctc gcttgccttg ctgagggtat
      301 aaattgccac agaaacagta ctggcaatta tgactgtcag caggataacc agggtacttc
      361 ccctgcggta caggagctct ttgataatca gtctcagtga attcatcata tatcttgttc
      421 agttttcagc ttaaaatatt taattcattt gcccggacct ttcaatacct gtttggcctt
      481 ttcttcaata catatactgt taaaacagca atgacaagta ttattatccc tcctgtcagc
      541 aggaaagaca gggtaaggga gttgtcttcc ttcatttcga gtttctccgg ctgataccgg
      601 ttcagaccat ctctctgatc ggtcagccat ttgggcagag gtctgtcatc aatagacctg
      661 tattgaaggg tatcctgctt catcagtgat gcagtaaggc agggttctcg tccagtatcc
      721 tgttaaccag tgccggaaga ggattttccc agttgtttgt gaacagaata tccgtgcccg
      781 gaagattgtc cttaataagg caactgcagt cagcagtcag gaaatcaatc atcaggttta
      841 tgttttctgt tgaaatcccc cttgccagaa gtggttcgag agccttgaac ctggcaaaga
      901 ttccgaacag catcggttcg tttatctctt tcaggtcgtt ttccacattc agaagcattg
      961 aaacaaaatg tttctcttcc gtactgcttc tgtcgagttc ccttacggac accacctctc
     1021 tgaacgggga tgctgcaact gttcttttta ttatctcaga ggcaggagca tccttttccg
     1081 ggtttccggt tttaagataa agcattacgc aaagcattcc cgacatcagt tcagaagcaa
     1141 tcttttccct caatggcgaa acggaaagat ttttaagctc cgcgtggtta ttgtatgttg
     1201 aaaacagcct gttcccgtaa atcagagcat aatagggttt ttctatgtca tttctcagta
     1261 tgctacggaa ttgaatatta gctgaattta tctccctgcc tatctgttca ttttgttttt
     1321 taaccggctc agtaacaggg tctttatgat aataaacaag caagtaatta ttggcttcgc
     1381 tgttaagcag cacccattcg gatacttttg tggtacatgc catgcttagt atgccggaca
     1441 gaaccaatat tctgagataa acaggtttca tcttattcag tatttacagg ctgtttnnnn
     1501 nnnnnncatt tccacacccg aaatgattgt atttccttca agaggtatca ggtccagccg
     1561 cagggttctt ccggccctga tccctgtaaa tgattttatt acttccttgt ctgttttccc
     1621 tgcttctgaa acaatatcaa aattccgcag tacttcctca ccctgcaggc tgatattcag
     1681 cacgcgctct cccggttttt tattctcaag ttctgaaaaa tacagtttaa ccgtgtacca
     1741 ggtttcagtc acatcctcat catctgcaat ggtaagctca aaggacctga ctccgcacag
     1801 agcactcgca cttacccagg gcgtaccgcg tgatacaacg gatataggct cctttcgtat
     1861 ccggtaataa ttcagcgtat ccatcctaac gggtattccc ggatcggaac ctgaaacgaa
     1921 gggatattcc atccacagtg tattgttttc ggaagttctg tccccgggtg cattgagatt
     1981 cagtccgaac tgcctgattt ttttaccatc ccatttatag ttgctgtttg tccagtatgt
     2041 catccagggc atatatacca gtgccaggga tgtctgattc tggtagggac actggcaggt
     2101 ccgggtataa tcgggtgaat taagaactcc gtcagcggca atcaggttgg tggagcagcc
     2161 tgccttccac cctccaaggc tgccggttcc ttcgaatacg tcgagattca cgaaaccggc
     2221 ggaagctgac cggaaggtca gaagatgttc acttgccgcc acaataccgc atccgtactc
     2281 acgcttgaaa ttccatttaa gatcctcctg tgtaagcggg tccttctcaa aaaggatttc
     2341 accggtcaaa aggtgaaaac cttcgccgtt ggtatagatt ttatcattat gtattatcgg
     2401 cggattggca tatttcagtg ctttatccca tatcttctcc cctgtaagga cattgtatgt
     2461 tatcatcctg tttccctcct caccatgaag catatccctt gaaggccgtg ttgcctgaag
     2521 taggagcctg tgttcagagg aataccccag ccatgttccg aagacatctt cagtgacctc
     2581 attcagtatc cggccgcttc tgatatcaac ataaagcagc ctggatccct ccgggggcga
     2641 ctctcccctc cttttcagct tcgtctcaac atattgggga agtttgtcaa gacaaaaaag
     2701 aatatcatca ccggctatta ctgaattatg aataaatccg tgattgcttt ccatgctcca
     2761 gatcctttct cccgtaaacc ggttcacgac tataagctca ttgctggcag taagattata
     2821 atcagtgaac ttcggatttt taatcttcat tgaatcattt tccagacccg tgtattcgga
     2881 aaagtttttg cccagaatca gaaggtcttc atatacacct atatagccca gttcctttgt
     2941 ctcttcatcg cccgtggtga atgattttat cagatctcct gttttaatat ccacaaaatg
     3001 gcaggtatct ccttcaataa cataaacata ttccctggtg gcaatgaaat tcgttccccg
     3061 tgcgtttgat ccgggcagat gcacctggtt gtattttgga tcaagcggat tctcctcatc
     3121 atacgattca tcataatata ccagccagaa atcatccttc aggctcctga aatccctttt
     3181 ccagagaacc ctgccggtat aaacgtcacg ggcacttata ccgttaaggc tttctataat
     3241 aagacgtccg tcaaccacct gctccccggg tccgtgcccg tgcctgggca aaacatccat
     3301 gtttgaattc cctccgaacc ataatattcc cagaggggct ctgacccgct tgtcatccga
     3361 cttgacagta ttggcaatat cgccgtaatt atgagtccag gccgaggaac cttcaaggga
     3421 tccttccctg gttatcatgg aataaagtct gtcagatttc attacagccc cgtacagatt
     3481 aaggctcagg aatgttcttt caagttcttc cctttccgtc ctgccggtcc tgataagaat
     3541 tttccccccg tagggacgtg ttgattcgta aatcatttcc aggaactcat tgtcaaaacc
     3601 gaagttctca ttgataaccg taagtgagga aaaatatttg ggaagaacag gatttggctt
     3661 ttcaaattcc aggaacataa gacggtctgc atgtattccg agtttgttaa aatgttccct
     3721 cagggcctcc accttctgcc ggtccttttc ataaaccact gttctgaggc ttgtttcttt
     3781 catcaggcct gataaaagtc ccatgtcgga accaaaaacc agtgcatatc cttcactgat
     3841 tccggcagaa ctgatgagac gctgaatttt acctgatacc ttccggggtc cggccactgc
     3901 tttttcatgt acaaggaccc tgtcagcagc tttatcacca aaagccatta tagtgccatc
     3961 atttgcaaca gcgataagtt tgccgttcga ggccaccagc cggtcaatga ctttttccgg
     4021 ggaatatttc cagaccagtt ccggttttcc gctgccggaa attcttacgg catatattcc
     4081 tgaactgtct gctgcataaa gacagttacc cgcctttatc aggtctgttc ccgcgggaac
     4141 gtcaatagtc cagagagtat ccagcttttt tgactcatca agaaatacac atgtaatgtc
     4201 tttccctgaa aaatagattt tatctccctc aataaccggg tattctccgg ccaccggaat
     4261 cagttttttc ccattttcgg aatcatacat gccggtaagc ctttcccggt gatgattgaa
     4321 aaacaccctg tcatttgcgc aggtaaaagc accacccgtc ttgccgcttg gagcgagctg
     4381 ataaaacagt ttttcacccg tggctttatc gaagccggcc ggaactgatc tgccgcccgc
     4441 aacaagaagc ctgtcaccgc tgatggtaaa gcatccctgc ggtgcaatat cagcaaatgc
     4501 cggtgaatta tggggctgga gaatatagtt gcttcctgtt ccttcatttt tccagattat
     4561 ttcaccatca gcggcattta ttgcataaat aaatgtaccc atcagcggaa aaatgcttga
     4621 agctgtataa atcacatcat cctgtattac aatgccgcct ctggcaggcc acattgagat
     4681 gaagcgctta ttgcccaaaa gcttgtttga cgccggtgca agcatttttt tccacaggag
     4741 ctgacctgat tctgcgctca gacaataaag gtatccgtca tctgaagtaa aaaaaatctt
     4801 ccctttgctg taggccaggg gcagacggac cgggccgtca gtaaaaaagt gc
//
LOCUS       DAOSPX010000253         4838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_15957_length_4838_cov_3.658582, whole genome shotgun sequence.
ACCESSION   DAOSPX010000253 DAOSPX010000000
VERSION     DAOSPX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15957_length_4838_cov_3.658582"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /gene="ftsY"
                     /locus_tag="PLZ75_11000"
     CDS             complement(<1..938)
                     /gene="ftsY"
                     /locus_tag="PLZ75_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOS72960.1"
                     /translation="MAFSLFFTRENKENLNKGLQKTRENVFVRLSRAVVGKSRVDDEV
                     LDNLEEVLVSSDVGVETTLQIIERIEKRVSRDKYLNVNELNTILKEEIVALLQDNRES
                     GETDFAADPGHKPLVIMVVGVNGTGKTTTIAKLAYNYRKNGKKVLLGAADTFRAAAIE
                     QLQIWADRAGADIVKQQMGSDPASVAYDTVKSAVAGNYDVAIIDTAGRLHNKINLMNE
                     LSKIRRVMDKILPGAPHEVLLVLDGSTGQNAYEQAKQFTAATNVTALAITKLDGTAKG
                     GVVIGISNLLKIPVKYIGIGEKMEDLQIFNREEFVDS"
     gene            complement(1033..1188)
                     /locus_tag="PLZ75_11005"
     CDS             complement(1033..1188)
                     /locus_tag="PLZ75_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOS72961.1"
                     /translation="MAKKAVAALKTGKGRVFTKCIKMTKSEKTGAYKFQEDIVHNDRV
                     SDFLDKK"
     gene            complement(1201..1389)
                     /gene="rpmG"
                     /locus_tag="PLZ75_11010"
     CDS             complement(1201..1389)
                     /gene="rpmG"
                     /locus_tag="PLZ75_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOS72962.1"
                     /translation="MAKKAKGNRLQVILECTEHKESGLPGMSRYITTKNRKNTPDRLE
                     RRKYNPVLKKHTIHREIK"
     gene            complement(1402..1644)
                     /gene="rpmB"
                     /locus_tag="PLZ75_11015"
     CDS             complement(1402..1644)
                     /gene="rpmB"
                     /locus_tag="PLZ75_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOS72963.1"
                     /translation="MSRICQVTGKKALSGNKVSHSKRRTKRKFYPNLFEKKYYLAEED
                     RWITLKVSAAGMRLINKNGLTAALKEAREKGYIVNY"
     gene            complement(1761..3002)
                     /locus_tag="PLZ75_11020"
     CDS             complement(1761..3002)
                     /locus_tag="PLZ75_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOS72964.1"
                     /translation="MKAWIITIGDELLIGQTVDTNSAFIGAELSKAGFDIERKISIHD
                     RRDDILQTFRGINPDVQLLICTGGLGPTSDDITKQTLCEVFDTRLVPDREVLTRVEEM
                     MSRRGFPMNENNRLQAMVPENCRVIPNEMGTAPGMLFEKDAAMFIFMPGVPFEMKHMM
                     TEHVIPILKERFNTQSIIHKNIMTYGAPEALLAEMLSAFESALPEELSLAYLPSSGVI
                     KLRLTASGKDRGMLEKIIGEQVAKLYQKIPDLIYGEDEKPLEKVVGELLAERKQTLST
                     AESCTGGKIAQMLTSVVGSSAYFMGSVVAYDNSVKRKLLGVPGELLDKHGAVSEEVVT
                     AMAEGVRRVTGTDYSVATSGIAGPDGGSPSKPVGTVWIAVSSDKGTVTEKYVYGSDRH
                     QNILRSSNAALNLLRLQIIRK"
     gene            3254..3613
                     /locus_tag="PLZ75_11025"
     CDS             3254..3613
                     /locus_tag="PLZ75_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOS72965.1"
                     /translation="MAQKFLILINDAPYGNEKAFNALRLAMQLEKDHVDTEVRVFLMA
                     DAAGCALANQNTPNGYYNIERMLKAVLAKGGKVKICGGCAEARGLKNASLIEGTEISS
                     MAELTNWVVESDKVITF"
     gene            3617..4240
                     /locus_tag="PLZ75_11030"
     CDS             3617..4240
                     /locus_tag="PLZ75_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS72966.1"
                     /translation="MIQNPFDICTEEYEKWFDENRITFESELLAIRQLLPQGRKGIEI
                     GIGTGIFAAELGIKYGIDPSENMLKLARERHLNVTRGFAENLPYPAESFYFAAFITSL
                     CFIDDPGRAMEEAHRILKDEGEIIIAFIDRDSQLGQTLLRLKEENKFYRIARFYSVPE
                     VIDIIEKHGFIVSGIVQTLTGPEAAVPEQPSEGYGKGGFVVIKARKK"
     gene            complement(4255..4764)
                     /locus_tag="PLZ75_11035"
     CDS             complement(4255..4764)
                     /locus_tag="PLZ75_11035"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009293029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOS72967.1"
                     /translation="MNRINIQYYKTSIGEMILGSFNRQLCIMDFRYRRMRSTIDRRIK
                     KGLEAEFTEQDDEVLREARKQLDEYLKGKRTAFDIPILMVGTDFQKEVWKALMQVPYG
                     RTATYSELAESINAKESVRAVAGANGANAIAIIIPCHRIIGSDGDLTGYGGGIAVKKR
                     LLNMERRNR"
BASE COUNT         1179 a         1157 c          998 g         1504 t
ORIGIN      
        1 gagtccacaa attcctcgcg gttgaaaatc tgcagatctt ccattttctc gccgatgcct
       61 atgtatttga cggggatttt cagaaggttt gaaataccga tcaccacgcc gcccttggct
      121 gtaccgtcga gtttcgtaat agccagtgcc gtaacatttg tggcagctgt gaactgtttt
      181 gcctgttcat aggcattctg gccggtggat ccgtcaagta ccagcaatac ctcatgcggt
      241 gccccgggca atatcttgtc catcaccctt ctgatcttgg aaagctcatt catcaggttg
      301 atcttgttat gcagccttcc ggcggtatcg ataatggcca catcataatt cccggccacg
      361 gcggatttca cggtgtcata tgccacggaa gcgggatcag agcccatctg ctgtttaacg
      421 atatccgctc ctgccctgtc ggcccatatc tgaagctgtt ctattgcagc tgccctgaag
      481 gtatcggctg cgcccagcaa tactttcttt ccgtttttcc tgtaattata tgcaagtttt
      541 gcaatagtgg ttgtctttcc cgtaccgttt acgccaacca ccattataac caggggctta
      601 tgaccaggat ccgctgcaaa gtcagtttcc ccggattccc tgttatcctg caggagagca
      661 acaatttcct ccttcaggat ggtgttcagt tcatttacat tcagatactt atccctggaa
      721 accctctttt ctatcctttc aatgatttgc agggttgttt caactcccac atcggaggat
      781 accaggactt cttcaagatt gtcgaggact tcgtcatcca ccctcgattt tcccacaaca
      841 gcccgggata gtcttacaaa aacattttcc cgtgttttct gaagtccctt gttcagattc
      901 tctttgtttt ctctggtgaa gaatagggaa aaagccattg ccggtaaata atatttagaa
      961 attatgtaaa aaaagctctc ttcacggatg aagaaagctt tgaatatcct tgcagtattt
     1021 tatctgttac ggctatttct tatccaaaaa gtcacttacg cgatcattgt gaacaatatc
     1081 ttcctggaac ttgtaggctc cggttttttc tgattttgtc attttgatgc atttggtgaa
     1141 tactctgccc ttacctgttt taagagctgc aacagctttc tttgccatga ttgaatattt
     1201 ttattttatt tccctgtgaa tggtatgttt cttcagcaca ggattatatt tccttctttc
     1261 gagcctgtca ggcgtattct ttctgttttt cgtggtgata tatctcgaca ttcccggaag
     1321 tccgctttcc ttgtgctcgg tacattcaag aataacctgc agtctgtttc ccttagcctt
     1381 ctttgccatg acctgttcct tttaataatt gacaatgtaa cctttttccc tggcttcctt
     1441 cagggctgcc gtcagtccgt tcttgttgat cagcctcata ccggcggccg acaccttaag
     1501 ggtaatccat ctgtcttcct cagccagata atatttcttt tcaaaaagat tgggatagaa
     1561 tttcctcttt gtccttcttt ttgaatgaga aaccttattg ccggacaacg cttttttccc
     1621 tgtaacctga caaatcctcg acattgattt tatgtttaat caaattaagc tatcaaaaat
     1681 gaagcgcaaa atacgtgatt tttatctgat taaccaaatg ttttaactga tattttcatc
     1741 aggcatgctg gtttttcaga ttatttcctg ataatctgca atctgagaag attcaatgcg
     1801 gcatttgagg atcttagtat attctgatgg cggtctgagc cgtaaacata tttttctgtt
     1861 accgttccct tatccgatga tacggcgatc cacactgtgc caacaggttt tgaagggctt
     1921 cccccatccg gaccggctat acccgatgtg gcgacggaat agtccgttcc ggttaccctt
     1981 ctgactccct ctgccatggc agtcacgacc tcttcactta cagccccgtg cttgtccagc
     2041 agttccccgg gaacccccag cagcttcctt tttaccgaat tgtcataggc caccacagat
     2101 cccatgaaat aggcagaact tcctacaaca gatgttaaca tctgtgcaat tttccctcct
     2161 gtgcagcttt cagccgttga aagagtctgt ttccgctcgg caagcagctc ccccaccacc
     2221 ttttccaggg gtttttcatc ttccccgtaa attaagtcag ggattttctg atacagcttt
     2281 gcgacctgtt ccccgattat tttctccagc atgcccctgt ccttgccact ggctgtcagc
     2341 ctcagtttta ttactcccga cgacggcaga tatgccaggc ttagctcctc cggaagagcc
     2401 gattcaaaag cgctgagcat ttctgccaga agggcttcgg gagcaccata tgtcattata
     2461 ttcttgtgga ttatgctttg tgtattgaac ctttccttca ggatcgggat gacatgttct
     2521 gtcatcatgt gtttcatttc aaagggaacg cccggcatga agataaacat ggcggcatcc
     2581 ttttcaaaca gcattcccgg tgctgttccc atttcattcg gaatcaccct gcagttttca
     2641 ggaaccatag cctgaagacg gttattctca ttcatcggaa atcccctgcg gctcatcatc
     2701 tcttcaaccc gcgtgagaac ctccctgtcc gggacaagac gggtatcaaa tacctcacaa
     2761 agagtttgct tggtaatgtc gtcacttgtc gggccaagtc cgccggtaca tatcaacagt
     2821 tgcacatcag gatttattcc cctgaatgtc tgcagaatat catccctgcg gtcgtgaatt
     2881 gaaattttcc tttcgatgtc aaatccggcc ttgctgagct ccgctcctat aaaggcagag
     2941 ttggtatcca ctgtctggcc tatcaggagc tcatccccta ttgttattat ccaggctttc
     3001 atcttgtttt tgttaaattc tacgtaattc tctgtaattc tgcgtaattc tacggcattc
     3061 cccgtaatga atcagtcttt ccccggatta aaacgcaaag aaacaagatt aatttattgg
     3121 taaatgaaca taaatgattt tttgttcggg aatattctgc ccgagccttc ttcctgtgat
     3181 gaaaataaat atcttttaat tttgattatt aggtacattt gcagcagata ataaaaaaaa
     3241 tatattttaa aatatggcac agaaattcct tattctcatt aatgatgctc cttatggaaa
     3301 tgaaaaggcg tttaatgctc ttcgactggc aatgcaactc gaaaaagacc atgttgatac
     3361 ggaagtgagg gtatttttaa tggccgatgc tgccggctgt gctctggcaa atcaaaacac
     3421 acccaatggt tattacaata ttgaaaggat gctgaaagca gtccttgcaa aagggggaaa
     3481 ggtgaaaatc tgcggcggat gtgccgaagc cagaggcctg aagaatgctt cgctgattga
     3541 aggaacagag ataagttcaa tggctgaact tacaaactgg gtagttgaaa gtgacaaggt
     3601 gattacattc tgattaatga ttcaaaatcc ctttgacata tgtactgagg aatatgaaaa
     3661 gtggttcgac gaaaacagaa tcacctttga gtcagagctc cttgccatcc gtcagctgtt
     3721 accgcagggc agaaagggta ttgagattgg cataggcacc ggcatttttg cagcggagct
     3781 tggaataaaa tacggaattg acccatcgga gaatatgctg aagcttgcaa gggaaaggca
     3841 tttaaatgtt accaggggct ttgccgaaaa tcttccttat cccgctgaaa gcttttattt
     3901 tgcagccttt atcacctccc tctgctttat agatgatccg ggaagggcca tggaggaagc
     3961 ccacaggata ttaaaggatg agggcgaaat catcatagcc tttattgacc gcgacagtca
     4021 gctgggtcaa acccttctgc gtctgaagga agaaaacaag ttttacagga tagccaggtt
     4081 ttattcagtt cccgaagtca ttgacatcat tgaaaagcat ggttttattg tttccggaat
     4141 agttcagaca ctgacaggtc cggaggccgc tgttccggaa cagccatcag aaggctacgg
     4201 caagggaggc tttgtggtga taaaagcaag aaaaaaataa gagcctgatc tgtttcagcg
     4261 gtttctcctt tccatgttca gaagacgttt tttgacggct atgccgccgc cgtagccggt
     4321 cagatcaccg tcactgccta taatccggtg acagggaatt attattgcaa ttgcatttgc
     4381 cccgtttgca ccggcaacgg cccttacgga ttctttcgca tttatgcttt ctgccagctc
     4441 actgtatgtt gcagtcctgc catagggaac ctgcatcagg gctttccaca cctctttctg
     4501 gaaatcagtg cccaccataa gtatcggaat atcaaaggcg gttcttttgc ctttaaggta
     4561 ttcatcaagc tgtttcctgg cttcccggag cacttcatca tcctgttcgg tgaattcggc
     4621 ttccagtccc ttcttgattc tcctgtcaat ggtggatctc attctccggt accggaaatc
     4681 cataatacaa agctgcctgt tgaaagatcc gagaatcatt tcacctatgc ttgtcttgta
     4741 atactgtatg ttaattctgt tcattcctgc cgtatcgttt tttattaaat ctttaatttc
     4801 tgtcatatta ttcattccct tcaaattggt tattcccg
//
LOCUS       DAOSPX010000254         4817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16047_length_4817_cov_4.761445, whole genome shotgun sequence.
ACCESSION   DAOSPX010000254 DAOSPX010000000
VERSION     DAOSPX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16047_length_4817_cov_4.761445"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLZ75_11040"
     CDS             <1..199
                     /locus_tag="PLZ75_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72968.1"
                     /translation="ADSIPFVYKRNKTRQIVILGDMNEKNVVVKQGLEPGTPIYIIPP
                     SNSSNFRLTGENLLAEIRQEK"
     gene            311..1747
                     /locus_tag="PLZ75_11045"
     CDS             311..1747
                     /locus_tag="PLZ75_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOS72969.1"
                     /translation="MADALKAPETPGNNKGNNKVPREYLFFLFGVLFVVSLITAADHF
                     NILKIGSRVLMIFRWIAIFAIIGFAWFRKSLTTWILISMIVGAEFGYDLPQVAAKMNL
                     VSKIFLRLIKTIIAPLLFATLVVGIAGHSNLKQIGRMGWKSLVYFEIVSTIALIIGLL
                     AINISRAGIGVNVPDTIDQSDIPHTEPITGSDLILHVFPENIAKSIYEGQVLQIVIFS
                     IIFGIAVAMIREKYRSPMLRFTESLAETMFKFTQLVMYFAPFAVFAAIAYSIGNMGLE
                     ILVNLFKLLATLYASLIIFLLVVLWPVAIMFKIPVRKFLSKIAEPVTIAFATTSSESA
                     LPIAMERMEEFGVPRKIVAFVMPTGYSFNLDGTTLYLSLATIFVAQISGIHLSWEKQL
                     LIVFTLMLTSKGVAGVPRASMVILLGTAASFGLPAWPIYIILGIDELMDMARTSVNVL
                     GNCLATAVIARWEGEFNPPPDPVRPIPA"
     gene            1824..2243
                     /locus_tag="PLZ75_11050"
     CDS             1824..2243
                     /locus_tag="PLZ75_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72970.1"
                     /translation="MNTVNKADISLTAALFAVLLFSSCINTRSSYTSPDEDRLMITRR
                     YAGDYIEYRNTDPDDFTGYDLIWIRTSQDSTYGKITAFGKTCDFVPGDRLFFRREYFS
                     PGGISGYWIYIIENDSTVSYKLTDYQHDRGVPVRSWF"
     gene            complement(2273..>4817)
                     /locus_tag="PLZ75_11055"
     CDS             complement(2273..>4817)
                     /locus_tag="PLZ75_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS72971.1"
                     /translation="LNEGDDTLYISNPSTSVLYKYPLSSAGVFSSDSKWAASRIGYSE
                     KQLEKMTEEKKTVKYKTRLLSLESGKERIFENTESFAFTKDAGHIILAGYANDNKTKD
                     IFIHNLKNGTTKNIGNVAEYAVNKAGNRLAYIISAADKSGNGVELMDLNNYNISFLDN
                     DTSVYRNLVWEKEGNALMFYKSFTDSTRLEENHIVFAVRNIYGKPEIRSFDPSSDTIR
                     FPGKLYVKESFKPQISDNCRIIYFGAHNWTVKEKNSKKSSEKPKIPGVDIWHWKDDPI
                     QPRQQKTYDTDRNFTYLFAWNPDKNSLVRLSGDSLRRTMLTGDGTNVLVMDDTPYRPA
                     FREAVSDYYLADPLTGKRKLLLQAFSSVAASSPAGKYVYYFMEKNWWVYDIRKDIHVN
                     LTGVLETVFWNTRDDSPRDEKPPFGTGGWLKDDKAIFVYDEFDVWKIGMDGSLPVRLT
                     NGRENNIIFRLVRLDTEYPYFSDKTDLYFSAFGDKTKESGFYRLTVKNKFEKLIFEPR
                     SVSALARAEKSDYFVYRSESYTESPNIYRVSASFRNPLRISDTNPQQAEFAWGKSEIV
                     NFTSRDGRPLQGALFYPAAYEEGKKYPMIVYIYEIRSNVLNNYVVPSPRSAYNTSNFT
                     GRGYFVYQPDIVYRTNHPGESAADCVIPAVEEVLKTGMIDAGRVGIMGHSWGAYQTSF
                     IITRTGLFSAAVAGAPLINMISMYNEIYWNTGTPNQNIFEISQGRLREPWWEQMENYM
                     ANSPMYGAQNINTPLLVTFGTSDGAVDWHQGIEMFTTMRRMEKPYIMLVYEGENHSLA
                     KKENQLDYTKKVAEFFDHYLLGTEPAEWILKGRKYLDKKTEEERVKEKK"
BASE COUNT         1150 a         1118 c         1102 g         1447 t
ORIGIN      
        1 agctgacagt attccctttg tttacaaaag aaacaaaacc aggcagattg ttattctcgg
       61 cgacatgaat gaaaagaatg tggttgtgaa acaaggcctt gagccgggta ctccgatata
      121 tataattcct ccttcaaatt cttcaaattt caggctcaca ggtgaaaatt tactggctga
      181 aatacggcag gagaaataaa tttttctttt tttaacttct ttttttacaa ttcctgtatc
      241 aggcaggggt ttttatgtgc caacctatcg cttcaattga ttatgtttgt aattctttta
      301 ccattatctt atggcggatg cactaaaagc cccggaaaca ccaggaaaca ataaaggaaa
      361 caacaaggta ccccgggaat atctgttttt tctgtttgga gttctctttg tggtctcatt
      421 aattaccgct gcagaccatt tcaatatcct taaaatagga agcagggttc tgatgatatt
      481 ccggtggata gccatatttg ccataatagg ctttgcatgg ttccgtaaat cgctgaccac
      541 ctggatactg atcagcatga tcgtgggagc ggagtttggt tatgatctgc ctcaggtggc
      601 agcaaaaatg aatctggtaa gcaaaatatt ccttcgtctt atcaagacta tcattgcccc
      661 cctgcttttt gcaacactgg tggttggcat agcagggcat tcaaatctta aacagattgg
      721 caggatggga tggaaatccc tggtctattt tgaaattgtg tcaaccatcg cacttatcat
      781 aggactgctg gcaatcaata tcagcagggc aggaatcgga gtcaatgtac ccgacacgat
      841 tgatcagtct gacatcccgc atacagaacc tatcacgggg agtgatctca ttcttcatgt
      901 ttttccggaa aacatagcca agtcaattta tgaggggcag gttctgcaga tagttatttt
      961 cagtatcatt ttcggcattg cggttgcgat gatccgggaa aaataccgga gtcccatgct
     1021 gagattcact gaatcgctgg cagaaacgat gttcaagttc actcagctgg taatgtattt
     1081 cgcgcctttt gccgtttttg ctgcaatagc atacagcata gggaatatgg ggctggagat
     1141 acttgtcaat ctgttcaagc tgctggccac cctctatgcc tccctgataa ttttcctgct
     1201 tgttgtgctc tggcccgtag ccatcatgtt caaaatacct gtgcgtaaat tcctcagcaa
     1261 gatagccgaa cccgtgacaa tagcatttgc gaccacaagt tccgaatcgg ctcttcccat
     1321 agcaatggag cggatggagg aattcggggt gccaagaaag attgtggcct ttgtgatgcc
     1381 caccggctat agtttcaatc tggacggaac aacactgtac ctgtcccttg caaccatatt
     1441 tgttgcccag atcagtggca tacacctttc atgggagaaa cagctgctga ttgtcttcac
     1501 cctgatgcta accagcaagg gagtggcagg agtaccgaga gcctccatgg tcatactgct
     1561 gggtaccgct gccagctttg gtctgccggc atggcccata tatattatcc tgggcataga
     1621 tgagctgatg gatatggccc ggacatcagt aaatgttctt ggcaactgcc tggctaccgc
     1681 tgtgattgca agatgggaag gggaattcaa tccgccgcct gatcccgtcc ggcccatccc
     1741 ggcgtgaaga ccggttgttt ccggtgattc ttgtttattt tgcggatttg aattattttt
     1801 gaattgattt atcttctgtc tgaatgaata ccgtgaataa agctgacatt tcgctgactg
     1861 cggcactgtt tgccgttttg ctgttttctt cctgcatcaa taccagaagt tcatatacca
     1921 gtcctgatga agaccggctg atgattacaa ggcgttatgc cggcgattat attgaataca
     1981 ggaatacgga tccggatgat tttaccggct atgaccttat ctggataagg acctcgcagg
     2041 acagcactta cgggaaaatc accgcattcg gaaagacctg cgattttgtt ccgggtgaca
     2101 ggcttttttt caggcgcgag tatttcagtc cgggaggtat ttcaggatac tggatctata
     2161 taatcgagaa cgattccacg gtttcataca aactcacgga ctaccagcat gaccggggag
     2221 tgccggtcag atcatggttc tagattcccc cggtccccga tccggtttct cctcactttt
     2281 tctcctttac cctttcctcc tctgtctttt tatcgaggta tttccttcct ttcagtatcc
     2341 attccgcagg ttccgttccc agaagatagt gatcaaaaaa ttcagcaacc tttttcgtat
     2401 agtcaagctg gttttccttt tttgccagcg aatggttttc cccctcgtaa acgagcatga
     2461 tataaggttt ttccattctt cgcatggttg tgaacatttc aatgccctgg tgccagtcga
     2521 cggctccgtc gctggtgccg aaggttacaa ggagcggggt gtttatgttt tgcgctccgt
     2581 acatggggga attggccata taattttcca tctgttccca ccagggttcc ctcagcctgc
     2641 cctgtgatat ctcaaaaata ttctggttcg gggttcccgt gttccagtat atttcattgt
     2701 acatgcttat catgttaata agcggggcac ctgccaccgc agccgagaaa agcccggtgc
     2761 gggtaatgat gaaactggtc tggtaggctc cccagctgtg tcccatgatt cctaccctgc
     2821 cggcatcaat cattccggtt ttcagtactt cctccactgc tggtatgaca cagtctgcag
     2881 cagattcccc gggatggttt gtacggtaga caatgtcggg ctgatagaca aagtaacccc
     2941 tgccggtaaa gtttgaggta ttgtatgcac ttctgggaga gggtacaaca taattattga
     3001 gaacatttga acggatctca tagatatata ctatcatcgg atatttcttt ccctcctcat
     3061 aagcggcggg ataaaacagt gctccctgca agggcctgcc gtccctgctg gtgaaattca
     3121 ctatttcact tttcccccat gcaaattctg cctgctgcgg attggtgtcg gatattctga
     3181 ggggattgcg gaaggaagca gaaaccctgt agatattcgg agattccgtg tatgactcgg
     3241 atctgtagac aaaataatca gatttctcag ccctggccag ggcgcttact gatctgggct
     3301 caaaaatcag tttttcaaat ttgtttttca cagtcagcct gtagaatccc gattccttgg
     3361 tcttatcgcc gaaagctgaa aagtacagat ctgtcttatc gctgaaatag ggatattcgg
     3421 tgtcaagcct gaccagtctg aaaatgatat tgttctccct cccgtttgtc agacgtacag
     3481 gcagggatcc gtccatgccg attttccaca cgtcaaactc gtcatataca aaaattgcct
     3541 tgtcatcctt cagccatccc ccggtaccga aggggggctt ctcatcgcgc ggactgtcgt
     3601 cgcgggtatt ccagaacact gtctcaagta cgcccgtcag gttaacatgt atatccttcc
     3661 ttatgtcata cacccaccag ttcttttcca tgaaataata tacatatttc ccggccggag
     3721 aggatgcagc cactgatgaa aaagcctgca gcagaagttt cctttttccc gtcaggggat
     3781 cggccaggta ataatcggaa acagcttccc tgaaagcggg cctgtacggt gtgtcgtcca
     3841 taacaagcac gtttgtcccg tcgcctgtga gcatggtccg cctgagactg tcgccgctca
     3901 gcctgacaag ggaattcttg tcgggattcc atgcaaacag atatgtgaaa ttgcggtccg
     3961 tatcataggt tttctgctgc ctcggctgaa tgggatcgtc tttccagtgc cagatatcaa
     4021 caccggggat ttttggtttt tcggaggatt ttttactgtt cttttccttt actgtccagt
     4081 tatgcgcacc gaaatagatt atgcggcagt tatcggaaat ctgaggtttg aatgattcct
     4141 ttacatataa ttttccggga aacctgatag tatcagagga aggatcaaag cttcttattt
     4201 cgggttttcc gtaaatattc ctgactgcaa atacaatatg attctcttca agccgtgtgc
     4261 tgtcggtaaa ggatttatag aacatcagtg cgtttccttc cttttcccac accagattcc
     4321 tgtaaacgga tgtatcgttg tccaggaaag agatgttata gttgttcaga tccatcagtt
     4381 caacaccgtt gccgcttttg tctgcagcac taattatgta tgccagcctg tttcccgctt
     4441 tattgacagc atactccgca acgtttccga tattctttgt agttccgttt ttcaggttat
     4501 ggatgaagat atcctttgtt ttgttgtcgt tcgcgtatcc tgccagtata atatggccgg
     4561 catcctttgt aaaggcaaag gattcagtgt tttcaaatat tctttcctta ccgctttcga
     4621 gactgagcag cctggtttta tatttcaccg tttttttctc ctctgtcatt ttctccagtt
     4681 gtttttcgct gtagccgatt ctgctggcgg cccattttga atcggaggag aaaacaccgg
     4741 cggaggagag cgggtattta tacaatactg atgtggaagg attactgata tacaatgtgt
     4801 cgtcgccctc gttaagg
//
LOCUS       DAOSPX010000255         4795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16133_length_4795_cov_3.218776, whole genome shotgun sequence.
ACCESSION   DAOSPX010000255 DAOSPX010000000
VERSION     DAOSPX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16133_length_4795_cov_3.218776"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="PLZ75_11060"
     CDS             <1..1828
                     /locus_tag="PLZ75_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOS72972.1"
                     /translation="DKWYSSGDPVFSVDGKYLFFTSERDFNPVYSQTEWNHAYVDMSR
                     IYLVPLAKDTPSPFAASDDKISEGEEAAPAETSPARGKKTAPAKSRESDSKNVIIDFD
                     GINDRIVSLPVRPGNYGNLQTVGDKIFYTSFSRSGGQQGAGYFDLKEKKEVDLGSGIS
                     LTPVPSGKKMLVRQGNRYGIIPVPSAKVELKETINTGDMKVLVDLSEEWENIFNESWR
                     QMRDFFYDPGMHGVDWKKMHEKYHVLLPHVSHRSDLTYVIGEMMGELSVGHAYVMNGE
                     RPQPERIQTGLLGARLSRDESGYARIDRILKGANWNQSLVSPLTVSGVDAKEGEYILA
                     VNGEPVNKVKDIYELLTGKAGVLVELTLNSKPDMAGSRNVIVKPLASEKELYYYNWVQ
                     ENTRKVTEATNGRVGYIHIPDMQVAGLNQFAEHYYPQLEKEALIIDDRSNGGGNVSPM
                     IIERLMRTMTYVTMNTGATVGDVNPGGTHLGPKVALCDKYSASDGDLFSYRFKFNKIG
                     TLIGTRTWGGVVGISGPAPTIDGGNINTPSWAPYAADGSSFIVEGYGVDPDIVIENDP
                     HREYLGQDDQLNKAIEVALEKLKTESRKVPEIPPFPDKSGRK"
     gene            complement(1839..2585)
                     /locus_tag="PLZ75_11065"
     CDS             complement(1839..2585)
                     /locus_tag="PLZ75_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOS72973.1"
                     /translation="MKDPYSKLVGILGTVIVHLIAAIIFMSFRVHDLQRDLSREFMVE
                     FEAIPDQENEEELIELPASALEKILQGDEELMNIARNLANRPVEQINPADYIDKVKEE
                     LIESGKLGADNYIDEQKRRAEAGPDENLAYEEKTDREKEEKEPDSAEMAANYQGPTRI
                     YYDLAGRTHQYLPIPIYKCRGAGKVVLRIAVNQKGAVESAVIIEDLSTTGDPCLVETA
                     VSTAKISRFNPDINAPRIQQGTLTYHFVAQ"
     gene            complement(2585..3274)
                     /locus_tag="PLZ75_11070"
     CDS             complement(2585..3274)
                     /locus_tag="PLZ75_11070"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS72974.1"
                     /translation="MNKIQNTVFFLFIFFVPVAGQAIQERFIQLSGIITDEAQNPVQG
                     VAVISKKLRRGSVSERSGIYSIATTPGDTVFFRILGYKRYHTVIPEDYTDRRCMVDIV
                     LESDTISIAEVTIFPWRNYDEFIKDVTKVKPADPLVEHMNENLASIYVAITNQSNLNI
                     TPEAGYRYSMEQNFTSMSGRNQYPVNNLLNPFAWSRFISEVKRGLLKNQSYKKPQQAK
                     VIKKKKKQERN"
     gene            3396..>4795
                     /locus_tag="PLZ75_11075"
     CDS             3396..>4795
                     /locus_tag="PLZ75_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOS72975.1"
                     /translation="MKRLLLLGDEAIAQAAIDAGLSGMYAYPGTPSTEIMEYIRASET
                     ARERNIHRTWSANEKTAMESALGMSYAGKRAMVAMKHVGLNVAADSFINSAITGVNGG
                     LIVVSADDPSMHSSQNEQDSRFYGKFAFVPCFEPSNQQEAYNMVFDAFELSEKYKTPV
                     LFRITTRLAHSRTSVSLVKYPVPEKELSLPGDPVQFVLLPAFARKKYKTLIATYESMR
                     YDARLSEYNFFRDGRRRELGIITCGIAYNYLMENLSETEPDFPILKICAYPFPPDLIR
                     EIMNRCDEILVLEEGYPLIEESITGICDSGKKVYGRLEGTVPRDGELNPSIVAKALGI
                     KSSQKRSVPAVVKMRPPSFCKGCGHSDTFNALIEAMKPYGPGRVFSDIGCYTLAALPP
                     YSIINSCVDMGASVTMAIGAADAGLSPSVAVIGDSTFTHSGMTGLLDAVTKNSPVTII
                     ISDNLTTGMTGGQESPG"
BASE COUNT         1342 a         1030 c         1149 g         1274 t
ORIGIN      
        1 tgacaagtgg tattcatcgg gtgaccccgt tttcagcgtg gacggaaaat acctcttctt
       61 cacttccgag agggatttca atcctgtata cagccagaca gaatggaatc atgcttatgt
      121 ggatatgagc agaatctatc tggttcccct ggcaaaagac acaccttcgc cctttgcagc
      181 ctctgatgat aaaatcagtg aaggagaaga agccgcgccg gccgaaactt ctccggcaag
      241 gggaaaaaag acagcccctg cgaaatcccg ggagagtgat tcaaaaaatg tcataattga
      301 ttttgacgga ataaatgaca gaatagtatc cctgccggtg aggccgggta attatggtaa
      361 cctgcagact gtcggggata aaatatttta tacttccttt tcccgttcag gcggtcagca
      421 gggagcaggt tattttgacc ttaaggagaa aaaggaagtt gacctgggca gcggaatatc
      481 actgacaccc gttcccagcg ggaaaaagat gcttgtcaga caggggaaca gatatgggat
      541 tattccggtt ccgtcagcaa aggtggagct gaaggaaacg ataaataccg gtgatatgaa
      601 agtactggtc gatctttccg aagaatggga aaatattttc aatgaatcat ggagacagat
      661 gcgtgatttt ttctatgacc ctggaatgca tggtgttgac tggaagaaga tgcatgagaa
      721 atatcatgta ttgctgccac atgtgagcca cagatcggat ctgacttatg ttatcgggga
      781 aatgatgggt gaactgagcg tgggacatgc ctatgtgatg aatggagaac gccctcagcc
      841 ggaaaggata cagacaggac tcctcggggc aaggctgagc agggatgaat cgggatatgc
      901 aaggattgac agaatactta agggagcaaa ctggaaccag tcgcttgttt caccattaac
      961 ggtcagcggt gttgatgcga aggaaggaga atacatactg gctgtgaacg gagaaccggt
     1021 aaataaggtg aaggatattt atgaactgct gacaggaaaa gccggggtgc ttgttgagct
     1081 tacacttaat tccaaacctg atatggccgg aagcaggaat gttatagtca aacccctggc
     1141 ttccgaaaag gaactttatt actataactg ggtccaggaa aacacccgta aagtcactga
     1201 agccacaaac ggaagagtgg gctatattca tattccggat atgcaggttg ccggactgaa
     1261 ccagtttgcc gagcattatt accctcagct tgagaaggaa gcacttataa ttgatgacag
     1321 gagcaatggc ggaggaaacg tttctcccat gataatagaa agactgatga ggactatgac
     1381 ctatgtaaca atgaataccg gggcaacggt cggcgatgtc aatccgggag gcacacatct
     1441 cggtcccaag gttgccctct gcgacaagta ttcggcatct gacggtgact tgttttccta
     1501 caggtttaaa ttcaataaaa taggtactct tatcggaacg agaacatggg gaggcgtggt
     1561 aggtatatca ggtccggctc ctacgattga cggtggcaat atcaatacac cctcatgggc
     1621 tccttatgca gcagacggaa gcagctttat tgtcgaaggc tacggagtgg accctgatat
     1681 tgttatagaa aatgatcccc acagggaata cctgggacag gatgatcagc tgaacaaggc
     1741 aatagaggtg gctcttgaaa agctaaagac ggagagcagg aaagttccgg aaataccgcc
     1801 atttcctgat aagagcggaa gaaaatagta aatcaccgct actgcgctac gaaatgatag
     1861 gttaaggttc cctgctgaat ccggggggca tttatatcgg gattgaatct tgatatttta
     1921 gctgttgata cggcagtctc aaccaggcag ggatcgcctg tcgtgctgag atcttctatt
     1981 atcacagccg actctactgc tcctttctgg ttaacggcta ttctgagaac aactttcccg
     2041 gctccccggc atttatagat gggaatgggc agatactgat gggttctgcc ggccaggtca
     2101 taataaatcc ttgtcggtcc ctggtaattt gcagccatct cagcagaatc aggctctttt
     2161 tcctcttttt ccctgtccgt tttctcttca tatgccagat tctcatcagg tccggcctct
     2221 gcccttcttt tctgctcatc tatgtaattg tcggctccca gctttccgct ttcaataagc
     2281 tcttccttaa ccttgtcaat ataatctgca ggattaatct gttcaacagg cctgttggca
     2341 agattccgtg caatattcat cagttcctcg tctccctgga ggattttttc aagagcggat
     2401 gccggtagtt caatgagctc ttcttcattt tcctgatccg gaattgcttc gaattcaacc
     2461 ataaattcac gggaaagatc cctctgcaga tcatggaccc tgaaggacat aaagattatt
     2521 gctgcaataa gatgaactat tacagttcca agaataccaa ccagtttgct gtaaggatct
     2581 ttcatcagtt cctctcctgt ttcttcttct tcttaataac tttcgcctgt tgtggtttct
     2641 tatacgactg attctttaaa agtcctcttt ttacttcaga aataaatctt gaccaggcaa
     2701 atggattcag gagattgttc acgggatact ggttccggcc tgacatgctt gtgaagttct
     2761 gttccatgga atagcggtaa cccgcttcag gtgtaatatt gaggttggat tggttggtta
     2821 ttgccacgta aatggatgcc aggttctcgt tcatgtgctc aaccagggga tcagccggct
     2881 tcacttttgt aacatccttt atgaattcat catagttccg ccatggaaaa attgtgactt
     2941 cagctatgga aatagtgtct gattcgagta caatatccac catacagcgc ctgtcggtat
     3001 aatcttccgg aatcacggta tggtatctct tatagcccag aatcctgaaa aatacagtat
     3061 cgcccggagt ggttgcaatt gaatagatgc cggatctttc acttacactt cccctccgga
     3121 gtttttttga aataactgca actccctgaa caggattttg tgcttcatcc gttataatac
     3181 cgctgagctg aatgaatctt tcctgtattg cctgtccggc aacgggaaca aaaaaaatga
     3241 aaaggaaaaa aacggtattt tgtattttgt tcaaaatgaa aaatcctaaa ctttaagcca
     3301 ttgaattctt atatatctgc aaagtaaaca ttattggatg taatgaaaaa gaattaacta
     3361 aattttaata aattgacaac cttaaaaaac agattatgaa aaggctcctt ttactgggag
     3421 atgaggctat tgctcaggct gccattgatg ccggactgag tggcatgtat gcttatccgg
     3481 gaactccgtc cactgaaatc atggaatata tccgggcttc agaaactgca cgggagcgga
     3541 atattcacag aacatggtcg gccaatgaaa agactgccat ggagtctgct cttggaatgt
     3601 cctatgcagg gaagagagcc atggtggcaa tgaaacatgt tggtctgaat gtggctgcag
     3661 acagttttat caattctgca attaccggtg taaacggtgg tctgatagtg gtttctgccg
     3721 atgacccttc aatgcattca tcacagaatg aacaggattc aagattttac gggaagtttg
     3781 cattcgttcc ctgctttgag ccctcaaacc agcaggaagc ttacaatatg gtttttgatg
     3841 catttgaatt gtctgaaaaa tacaaaaccc ctgttctgtt cagaataacg acccggcttg
     3901 cccactccag gacaagtgtc agtcttgtaa aatatccggt tccggagaaa gagctgagtc
     3961 tgcccgggga tcccgttcag ttcgtactgc ttccggcatt cgcgagaaaa aaatacaaaa
     4021 ccctgattgc cacttatgaa tccatgagat atgatgcaag gttgtcggag tataattttt
     4081 tcagggatgg aaggagaaga gaacttggaa tcatcacctg cgggattgca tacaattacc
     4141 ttatggaaaa cctttcagaa acggagccgg attttcccat actgaaaatc tgtgcctatc
     4201 cctttccccc ggacctgata agggagatta tgaacagatg tgatgaaatc ctggttcttg
     4261 aagaaggcta tccccttatt gaagaaagta ttaccgggat ctgtgacagc ggaaaaaagg
     4321 tttacggacg gcttgaaggc actgtgccga gagacgggga actgaatcct tccattgtgg
     4381 caaaagctct gggaataaaa agctcacaaa aaagaagtgt gcctgcagtc gtaaaaatga
     4441 gaccaccttc attctgcaag ggctgcgggc attccgatac tttcaatgca ctgattgaag
     4501 caatgaagcc ctacggaccg ggcagagtct tttcagacat tggatgctac accctggcag
     4561 cactgccgcc ctatagcatt atcaactcat gtgtggatat gggcgcttcg gtcacaatgg
     4621 caatcggagc tgccgatgcc ggattgtcac cctcggtagc cgttattggt gattccacct
     4681 ttacacactc cgggatgacc ggcctgcttg atgccgttac taaaaattca cctgtaacga
     4741 taattatttc cgataatctc acaaccggca tgaccggcgg ccaggaatca cccgg
//
LOCUS       DAOSPX010000256         4791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16153_length_4791_cov_4.004645, whole genome shotgun sequence.
ACCESSION   DAOSPX010000256 DAOSPX010000000
VERSION     DAOSPX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16153_length_4791_cov_4.004645"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLZ75_11080"
     CDS             <1..353
                     /locus_tag="PLZ75_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOS72976.1"
                     /translation="PDDTLGDLVKAISKSRRNIFPVVDVYNILEGVVILDDVREIMFN
                     TGLYDVTFVRDLMTIPPSYIDKEEDIETVMETFRKTGAWNLPVLDKGYYAGFLSKSRI
                     FSTYRELLVEFTGE"
     gene            372..1652
                     /gene="glyA"
                     /locus_tag="PLZ75_11085"
     CDS             372..1652
                     /gene="glyA"
                     /locus_tag="PLZ75_11085"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOS72977.1"
                     /translation="MNRDKKIFDIIERERQRQLNGIELIASENFVSDQVLQAMGSVLT
                     NKYAEGYPGRRYYGGCEVIDEAEEIAISRLCTLFGAEYANVQPHSGAQANMAVFLSVL
                     KPGDTFMGLNLAHGGHLTHGSPVNSSGMLYRPVSYGVREDTGLVDYDMMEELALKEKP
                     RLIVGGASAYSREWDYERMRALADRIGAIFMVDMAHPAGLIAAGLLENPVKYAHIVTS
                     TTHKTLRGPRGGIILIGKDFENPWGIKTTKGETRMMSSLVNSAVFPGVQGGPLVHVIA
                     SKAVAAGEALDPEFRNYQGKVRRNAFVMAESFIEKGYKVISGGTDNHLMLIDLRTKFP
                     AISGKKAENTLVRAHITVNKNMVPFDTRSPFLASGLRVGTAAITTRGMDEEHMPLIVD
                     LIDEVLSNIDNEDVIEKVGKKAIQLMKPFPLFAY"
     gene            1703..2092
                     /locus_tag="PLZ75_11090"
     CDS             1703..2092
                     /locus_tag="PLZ75_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS72978.1"
                     /translation="MVKKRLIFIVDDDPFINKLIVNKFTREGFEVRAFESGEGCIDNI
                     GDNPGLIILDYFFTGSSELYPDGMVIFNKIRELKPEIPVIMLSGQESGELVLEFARKG
                     IADYIIKDSNLTDNLSAAVSDVFAREG"
     gene            complement(2171..3109)
                     /locus_tag="PLZ75_11095"
     CDS             complement(2171..3109)
                     /locus_tag="PLZ75_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOS72979.1"
                     /translation="MRPHPNNDLYRIPSERNKGIIGTAVIHAVALLLLIVLGFSVPPP
                     PESEEGILVNFGTGETGFGAVEPSPPPSRTETSPPPPPPPAAVPQTVSGEQALLTQDI
                     EEAPEVKKVDPAAEKRRLEQLEAEKKRRAEMEAERIRREIEAERIRKEREEAERIRRE
                     KEAEEKRMAEIKSRTMGALEGSKNAGTTSKSEGIAGGAGNQGSPTGSVDSRNRGEGSG
                     LGDKGVSYDLGGRGVQRLPLPEYIYQTEGRVVVEIRVDRSGKVIAANPGAKGSTSLDE
                     NLLKLAKSAAEKAQFNPDPDAPFTQVGTITYIFKLN"
     gene            complement(3090..3482)
                     /locus_tag="PLZ75_11100"
     CDS             complement(3090..3482)
                     /locus_tag="PLZ75_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOS72980.1"
                     /translation="MALSQRNKISISFSSTGMTDVVFNLLIFFMLTSTLVHPTALKLL
                     LPKGSAQTAAKPQTTVSITADQRYYVEQQPVSLAELEAALRQKLGSNPDTYISLHADK
                     NVPFESVVEVLNIAQANNYKLIIATTPK"
     gene            complement(3491..4174)
                     /locus_tag="PLZ75_11105"
     CDS             complement(3491..4174)
                     /locus_tag="PLZ75_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOS72981.1"
                     /translation="MGFLQFAMQDVSLPVETTVDSHMTLLDFAMKGGWVMVPIVLLSF
                     VAAYIFIERFYVIKKASQQDMSFMNRIKDYIHEGRIEAALALCSSTDSPAARMIEKGI
                     NRLGRPLHDISTAIENAGKLEISKLEKSFPTLATITGAEPMLGFLGTVIGMVKSFYAM
                     SQAGSNIDVSLLSDGIYTALITTVAGLSVGILGYFAYNTLVVRVEKVVFTMEATLTEF
                     MDILNEPVK"
     gene            complement(4317..>4791)
                     /locus_tag="PLZ75_11110"
     CDS             complement(4317..>4791)
                     /locus_tag="PLZ75_11110"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOS72982.1"
                     /translation="GTSAMAVTTTEMLSHQLSNWLSQISNDFDIGFVYRPGYKDINAQ
                     EVEVALSTQLLNDRVTINGNFDVRGAEKAAGTSDKITGDFDIEYKLTEKIRFRFFNRF
                     NNPYTGKGAPYTQGFGLFFRKDFNQFADLFRKTGKSEMKKEDEVMIEGEGPEASR"
BASE COUNT         1186 a         1092 c         1130 g         1383 t
ORIGIN      
        1 aacccgacga taccctgggt gacctggtaa aggcaatatc gaaatccaga cggaacattt
       61 tccccgttgt tgatgtttat aacatactgg aaggtgtggt cattcttgat gacgtgaggg
      121 aaataatgtt caacaccggt ctgtatgatg tgacctttgt cagggatctt atgacaattc
      181 ctccctcata tatcgacaag gaagaggata ttgaaacggt tatggaaacc ttcaggaaaa
      241 cgggagcatg gaaccttccc gttcttgaca agggttatta tgcaggattt ttatccaaat
      301 caagaatttt ctcaacttac cgtgaattgc tggttgagtt taccggggaa taataattta
      361 aaaacaagta tatgaatagg gacaaaaaga tttttgacat aattgaaaga gagcgtcagc
      421 gtcagctgaa cgggattgaa ctgatcgcat ccgagaattt tgtgagtgac caggttctgc
      481 aggcaatggg atcggtactt acaaacaaat atgccgaagg ctatccggga cgcagatatt
      541 acggcggttg cgaggttatt gatgaagccg aggaaattgc gataagccgg ctttgtacat
      601 tgttcggagc ggaatatgcg aatgtgcagc ctcattccgg ggcacaggcg aatatggctg
      661 tattcctgag cgtgcttaaa ccgggcgata cttttatggg acttaatctg gctcacgggg
      721 gacatcttac ccacggatct ccggtcaatt cctcgggaat gctttaccgt cccgtgtcat
      781 acggtgtacg tgaggatacc ggccttgtgg actatgacat gatggaagaa ctggcactga
      841 aggagaaacc caggctgata gtcggaggtg cttcagcata ttcacgcgaa tgggattatg
      901 aaagaatgag agctctggcc gacaggatcg gtgcaatttt catggttgac atggctcatc
      961 cggccgggct cattgctgcc ggactgcttg aaaatcccgt gaaatatgca catattgtca
     1021 cgtcaacaac gcataagaca ctcaggggac cacgtggcgg catcatcctc attggcaagg
     1081 attttgaaaa tccctgggga atcaagacaa cgaaaggaga aacaagaatg atgtcgtcgc
     1141 ttgtgaattc ggcagtattc cccggagtcc agggaggtcc gcttgtacat gtgatagcat
     1201 caaaagccgt tgctgccggt gaagcccttg atccggaatt cagaaactac cagggaaagg
     1261 taaggagaaa tgcattcgtg atggcagaaa gctttattga aaaagggtat aaggtgatct
     1321 ctggcggcac tgacaaccat ctcatgctga tagatctcag aaccaaattt cccgccattt
     1381 cgggaaaaaa agccgagaac accctggtga gggcccatat taccgtaaac aagaacatgg
     1441 taccctttga cacccgctcg cccttccttg cttccggtct cagagtggga acagctgcca
     1501 taacaacacg gggaatggat gaggaacaca tgccgcttat tgttgatctg atcgacgagg
     1561 tgctttcaaa tattgataat gaggacgtta ttgagaaagt tggaaaaaag gccattcagc
     1621 tgatgaaacc tttccctctt tttgcctatt gattgttatt ttttgttatc tttaaataat
     1681 tcagttaaat atatgccctc agatggttaa aaaaaggctg atctttattg ttgatgacga
     1741 tccttttatc aataaactga tagttaataa attcacccgg gaaggatttg aggtcagggc
     1801 ctttgagtca ggagagggct gtatagacaa tattggcgat aatcccggac ttatcattct
     1861 tgattatttt tttaccggca gcagcgagct ctatcccgac ggcatggtta ttttcaacaa
     1921 gatcagggag ctgaaaccgg aaatacccgt cattatgctt tcaggccagg aaagcggtga
     1981 actggtgctt gaatttgcca ggaaaggtat agctgattat ataatcaagg acagtaatct
     2041 gacagataat ctctctgctg ctgtcagcga tgtatttgcc agggagggct gagatttgtt
     2101 aaaaccagta ataacggctt tctctcagcg tcagtgagac ggcagtatga aatctgtctt
     2161 ttttaagggg ctagttcagc ttgaagatat aggtgattgt tccgacctga gtgaatggtg
     2221 cgtcaggatc ggggttgaac tgagctttct cggcagcact ttttgcaagc ttaagcaggt
     2281 tttcatcaag tgatgttgat ccctttgctc ctggatttgc cgctattacc tttcctgatc
     2341 tgtcgactct tatttccacc acaaccctgc cttccgtctg gtaaatgtat tccggcagcg
     2401 gaagtctctg cacgcctctt cctccgagat cataggatac acccttgtct cccagtccgc
     2461 ttccttcacc ccggttccgg gagtcaaccg agccggtggg tgaaccctgg tttccggctc
     2521 ctccggcaat gccttcactt ttggaggtgg tgccggcatt tttggatcct tcaagagcac
     2581 ccatggtacg gctttttatt tctgccatcc tcttttcttc agcttccttt tctcttcgta
     2641 tgcgctctgc ttcctccctt tcctttctta tgcgttcagc ttcaatctcc cttcttatcc
     2701 gttctgcttc catctcggcc cttcttttct tttctgcctc cagctgttca agccgtcttt
     2761 tttcagctgc gggatccacc tttttaactt ccggtgcttc ttcaatatcc tgggtcagaa
     2821 gcgcctgttc cccggaaacg gtctgcggta cggctgccgg aggcggagga ggaggcggtg
     2881 aagtctccgt ccttgagggc gggggagatg gttctactgc cccgaatcct gtttcacccg
     2941 tgccaaaatt aaccaggatg ccttcctcgc tttccggggg aggtggtacg gaaaagccca
     3001 ggactatcag caataacagt gccaccgcat gtattacggc tgttccgatg attcccttgt
     3061 tcctttcgga aggaatcctg tagagatcgt tatttgggtg tggtcgcaat tattaattta
     3121 taattgtttg cctgtgctat gttcaatact tccacaacac tttcaaaagg aacattcttg
     3181 tctgcgtgaa gtgaaatata tgtatccgga ttgcttccga gcttctgtct gagggcagct
     3241 tccagctctg caaggcttac gggctgctgt tcgacatagt atctctggtc tgcggttatt
     3301 gaaacagtgg tctggggctt tgccgctgtc tgtgccgatc ctttcggcag aagaagttta
     3361 agagctgtcg gatgaacaag tgttgaagtc agcataaaga atatcagcag gttgaacaca
     3421 acgtcggtca ttcctgtgga ggaaaagctg atgcttatct tatttctctg ggataaagcc
     3481 atttctgatc ttatttaacc ggttcattca aaatatccat aaattccgtt agtgtggctt
     3541 ccattgtgaa gacaaccttt tcaaccctta caacaagcgt gttataggca aaatatccga
     3601 gtatccccac agacagtcct gcaacggttg tgatcagtgc agtgtagatt ccgtctgaaa
     3661 ggagggaaac gtcaatattg cttcctgcct gtgacattgc gtagaatgat ttcaccattc
     3721 ctataaccgt tccgaggaat cccagcattg gttcagctcc ggttatggtg gcgagagtgg
     3781 gaaagctttt ctccagtttt gatatttcaa gcttcccggc attttctatt gccgtactga
     3841 tgtcatgcag tggtcttccg agcctgttta ttcctttctc gatcatcctt gctgccggac
     3901 tgtcggtgga gctgcagaga gcaagtgcag cctcaatcct tccctcgtgt atataatcct
     3961 tgatccggtt catgaaagac atatcctgct gcgatgcctt ctttatcaca taaaatcttt
     4021 caataaaaat ataggctgca acaaaggaca ataacacgat gggaaccatt acccatccac
     4081 ccttcattgc aaaatcgaga agcgtcatat gcgaatcaac tgtcgtttcg acaggcaggc
     4141 tgacatcctg catggcaaac tgtaaaaatc ccatcttaat ttccgtattt ccgtttaaat
     4201 ttattaatct gttatttaag ctgcgaatat attaaaaaac gtattgatac agccttatag
     4261 tatttcctgt taataacaaa ggtccggttt ttattaaccg gaaactgttt cctcagctag
     4321 cgggaagctt ccggtccttc accctcaatc attacctcat cttccttttt catttcagat
     4381 tttccggttt ttctgaacag gtcggcaaac tggttgaaat ccttgcggaa aaacaaaccg
     4441 aatccctggg tatagggagc tccctttccc gtatacggat tattgaatct gttgaagaac
     4501 ctgaacctga ttttttcggt cagcttgtat tcaatatcaa aatccccggt aatcttatcc
     4561 gatgtgccgg cagctttttc tgcccccctt acgtcaaaat tcccgtttat tgtaaccctg
     4621 tcgttgagaa gctgggtgga caatgccact tcgacttcct gtgcgtttat gtccttgtat
     4681 cccggacggt acacgaagcc gatgtcaaaa tcattgctta tctgggacag ccagttgctc
     4741 agctggtgtg acagcatctc ggtggttgtc acggccatgg cggaagtgcc g
//
LOCUS       DAOSPX010000257         4788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16159_length_4788_cov_5.035918, whole genome shotgun sequence.
ACCESSION   DAOSPX010000257 DAOSPX010000000
VERSION     DAOSPX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16159_length_4788_cov_5.035918"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..679
                     /locus_tag="PLZ75_11115"
     CDS             380..679
                     /locus_tag="PLZ75_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOS72983.1"
                     /translation="MSLDWDNYGARFYDPAIGRWHSVDPLAEKYGRWSPYNYVLNNPL
                     KFIDSDGRKVEFAPGSSQEFKNKGGILLLYSPFKSFLVYRFRNQNPAGNRVLFYL"
     gene            complement(670..924)
                     /locus_tag="PLZ75_11120"
     CDS             complement(670..924)
                     /locus_tag="PLZ75_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOS72984.1"
                     /translation="MKYTVLIERYAQKQIMRLDKKAIPVIKSAIASLADNPRPKGYKK
                     LKGEDAYRIRVGDYRVIYEIDNGKIIVTVVSVGHRKDIYK"
     gene            complement(921..1118)
                     /locus_tag="PLZ75_11125"
     CDS             complement(921..1118)
                     /locus_tag="PLZ75_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72985.1"
                     /translation="MIQEKFVIDKNGRKIAVQVPIKTYEKLVADSEELEEIMEYRKAK
                     TNKGEPIPFEQAFSEIEKAMR"
     gene            complement(1132..1467)
                     /locus_tag="PLZ75_11130"
     CDS             complement(1132..1467)
                     /locus_tag="PLZ75_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS72986.1"
                     /translation="MTLGQHITTLRKAKKLSQSELGKEVGTSGDIIGRYERDEVKPSI
                     EVIIKIADALEISIDYMVGKTDFELDKDTLRRIQDVSTLPVEEKKQVFMVIDALIRDF
                     KTRKAYATH"
     gene            complement(2351..3295)
                     /locus_tag="PLZ75_11135"
     CDS             complement(2351..3295)
                     /locus_tag="PLZ75_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOS72987.1"
                     /translation="MIKSFGKIMAIATSRVKGKGEIQINEDLCTGCAECVEVCKDFSL
                     TIKDNKASVSDNPLFGCIACGHCMAVCPADAIHVSGRMISRDDLFELPHADEAASYEQ
                     LTNLFLRRRSIREFLDKPVEPGKIEKILDAAMTSPMGIPPSDVNVLILNSKEKNRLFI
                     KDFCNYLNSIRWMVSGWFLTLMRPVWGKTNADLFQSFVKPLFEIYISSMNKGINSVTY
                     DAPLVMYFYGSPYCDPADPVVAATTAMYAGQSLGLGTCMLGGIHPLIQYGSGARKFRE
                     KYGIKYTSREGLFVIFGYPDADYKKGIRRSFASTTFLQ"
     gene            3734..>4788
                     /locus_tag="PLZ75_11140"
     CDS             3734..>4788
                     /locus_tag="PLZ75_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOS72988.1"
                     /translation="MFFKKYLAEEIARPVWTPAVMTVNDLFRSFSKLQQAENEILIFE
                     LYNSYKKVSKQVESFDDFYFWGDMLLNDFDDVDKYLSDALTLFRNISDLKKIDQEFGG
                     LTPEQVEIIRRFWTNIDPGKLTREKSGFLNIWSILYDLYVDFRDSLKSRNIAYEGMIF
                     RDVAESYVDGIISGLQWETLHFAGFNALNRCETTLLTALKKAGKGRFYWDYDESYISG
                     GRFSSAGYFIRENIAVFGNDMPGDWNYNTFLSRETAGINRRIINTSSDMAQVKLIPGL
                     ISKLPGFESENAHETAVILADETLLIPFLSSLPENSGNINITMGFPLRHTAVYAFIRR
                     IMEMQVRARSENGTILF"
BASE COUNT         1413 a          959 c          951 g         1465 t
ORIGIN      
        1 agattgacct tataactgaa acttcactga aaaatgaaaa gctaagaaaa tacatttata
       61 aagatctgaa gatcattttt gaatgaaaga tgacaaaatc tatattgaac atattctcag
      121 gagcgttgac aggataaatt cctacctcgg aggtaaagac tatcagtttt tctaaaaaga
      181 ctttttaact caggatgccg ttatacgcta gcttgaaatc attggagaaa ccaccaggca
      241 tgccctggtc cgacatggta ggcatgagaa atattttaat tcatgattat atagatgttg
      301 acacgggtat tatctgggaa actgtttcaa cgagtttgcc agaactccaa aagatgcttg
      361 aaacccttaa gtggcttatg tgagtcttga ctgggataat tacggagcaa ggttttatga
      421 tccagctatc gggaggtggc attcggtgga tccgttggcg gaaaaatacg gaagatggag
      481 tccttataac tatgttctaa acaatccttt aaaatttatt gatagcgatg gaagaaaggt
      541 cgaatttgcg ccaggatctt ctcaggagtt caaaaacaaa ggaggtattt tattattata
      601 ttctcctttt aaatcttttt tagtgtacag atttagaaat caaaacccgg ctggcaaccg
      661 ggttttgttt tatttgtaga tatccttcct gtgtccgaca gaaacgacgg taacgataat
      721 tttaccgttg tcaatttcgt agataaccct gtaatcaccc accctgatac ggtatgcatc
      781 ttcacctttg agttttttat agcccttcgg acggggatta tcggcaagcg aggcaatagc
      841 agatttaatg accggaatag ctttcttatc cagcctcatt atctgttttt gtgcgtaacg
      901 ttcaatgaga acggtgtact tcatctcatg gctttttcaa tctcgctgaa agcctgttca
      961 aacgggatag gctccccttt attggttttt gccttacggt attccattat ctcttccagt
     1021 tcttcggaat cagccacaag tttctcgtaa gtcttaattg gaacctgtac ggctatttta
     1081 cgaccatttt tatctataac gaatttttcc tgaatcatac tgtaatttta tttaatgtgt
     1141 tgcataagct ttacgggtct tgaaatccct gatcagggca tcaattacca taaatacctg
     1201 ttttttttct tcgacaggca gtgttgagac atcctgtatt ctgcgcaaag tatccttatc
     1261 gagttcaaaa tctgttttac ctaccatgta atctatagat atctccaggg catcggcaat
     1321 ttttataatc acttctatgg atggttttac ctcgtccctc tcataacgcc cgataatgtc
     1381 cccggaagtt cccacttcct tacccaactc gctttgagag agtttttttg cttttctgag
     1441 cgtagtgatg tgttgtccta gtgtcatact tatatatcgt taagatgatg taaataatat
     1501 gaattatgca aaaataattg ataaatatat caaaaacaag aaatgttttc tcgacattct
     1561 ctgatatata ttttagattt gtgaccgata tgtcagaaaa tagttaagaa aatatttttt
     1621 aacaatggaa atctccgaaa taaaacaatg tcttttcatt acaatcgtcc tgagacatta
     1681 caacctccaa tccgaccggc accacttttt gaaatgtccc ttccatcagg atgaagatcc
     1741 cagcctgaag aaataatcca gaaaccattg gcacattaaa gcgaacctga acatgataaa
     1801 gaacttcttc caaggaacaa agttaataca aagcccttta gcatcactcg tgaaaaaatg
     1861 gaaactgcta ccgcactaat tgtcacaagt cagggaaagt aataagaaag ataaatgtca
     1921 atgtaaattg aatataagtt aaaagcctcg gcaagtccgg ggctttttgc attaaacctt
     1981 aaacggattt accattttta agccattgat attgttaaag tccttttcgt tgttggtgat
     2041 aagcgtaaaa ccatgagcca gggcagtagc ggcaataaaa tcttttaccc gttgttcttt
     2101 ggctgtatcc gttttccagc aaagcagttc tgtgtaaaat gcacttcgat aagttctgcc
     2161 agtgcgcttt gtgaaatccc tttttgcttg cgaatttgct aaacattgtg aattaacaaa
     2221 ttatattatt aatatatata gttaaatatc aacaacgtaa gtggatttta agggtcgact
     2281 agttactgcc aggatcaaac agcagtactc cggttatttt aaagtatttc gcttcttgtt
     2341 cgtttggagt ttattgcaga aatgtggttg atgcaaaaga tcttctgatg ccttttttat
     2401 aatctgcatc cggatagcca aaaattacga ataaaccttc tctgctcgtg tatttaattc
     2461 cgtacttttc tctgaatttc cttgccccgg atccatactg aattagagga tgtattcctc
     2521 cgagcataca ggttcccagt ccaagagatt gaccggcata catagccgtc gtggcagcaa
     2581 caacagggtc tgcaggatca cagtaggggg aaccgtaaaa atacatcaca agcggggcgt
     2641 cataagtaac cgaattaata cccttgttca tactggaaat ataaatttcg aacaatggct
     2701 taacaaagct ctgaaacaaa tcggcatttg tcttccccca aacgggacgc atcagagtta
     2761 aaaaccatcc ggaaaccatc catcgaatac tattcaagta gttgcaaaaa tctttgataa
     2821 ataatctgtt cttttcttta ctgtttaata tcaaaacatt tacatcagaa ggtggtatgc
     2881 ccattggcga tgtcatggcg gcatcaagta ttttctcaat ttttccgggt tcaaccggtt
     2941 tgtcaaggaa ttctcttata cttctgcgtc tcagaaacag gtttgtcaat tgttcataac
     3001 tggctgcctc gtctgcatgc ggcagttcaa acaaatcatc ccttgaaatc atccttccgc
     3061 tgacatgaat tgcatctgcc ggacacactg ccatgcaatg accgcaggca atacacccga
     3121 agaggggatt atcactgaca gaggccttat tgtccttgat tgtaagacta aaatccttgc
     3181 atacttcaac acattctgca caacctgtgc ataaatcctc gttgatttgt atttctccct
     3241 ttccttttac tctggatgtt gctattgcca taatctttcc aaatgactta atcaattgac
     3301 tattttattt tccggctgat gtgcaatagc aaatgtatgt actttatacc ttcttagaat
     3361 ccttttttcc atacttttga ggaatgaaga agagttatta cattgtcttc tgactggaac
     3421 ggaattcttg agttcctgaa atccggagct ccatagtatg gctgttccgg gatttcgttg
     3481 ttttgaagtt tcggagtata actgttacgg agatccggac cccggctgtt ccggagctcc
     3541 cgcggaaaat acctgaaaag acagaccgat ttgtaatagt ataaatgcaa aagctgaaca
     3601 ttgtaagtaa gcaggtaaat gaaataaatg aaagatggaa tattttctcc ggcgtatagc
     3661 ccgacatctt tattctcaat atggaaacag cctccataat cactgtcttg ttttcccgaa
     3721 cagaagagcc ggattgtttt ttaagaaata tcttgctgaa gagatagcca ggccggtgtg
     3781 gacaccggca gttatgacgg taaatgatct tttccggtcg ttttcaaaat tacagcaggc
     3841 ggaaaatgaa atactgatct ttgaattgta taactcatat aaaaaggtaa gtaagcaggt
     3901 tgaaagcttc gatgatttct atttctgggg cgacatgctc ctgaatgatt ttgatgatgt
     3961 tgataaatac ctgtctgacg ctttaactct tttcagaaat attagcgacc ttaaaaagat
     4021 cgatcaggaa ttcgggggac ttactccgga acaggttgag ataatcagac gcttctggac
     4081 aaacattgat cccggcaaac taacgaggga aaagtcaggt ttcctgaata tctggtcaat
     4141 tctgtatgac ctgtatgtgg atttcaggga ttccctgaaa tccaggaata tcgcatatga
     4201 aggaatgata ttcagagatg tggctgagag ctatgtcgat gggatcatat ccggccttca
     4261 gtgggaaaca ctgcattttg caggtttcaa tgcccttaac cgctgcgaaa cgactcttct
     4321 cacagccctg aagaaggccg ggaagggcag gttttactgg gattatgacg aatcctatat
     4381 cagcggcggc agattcagct ccgcaggata tttcatcagg gaaaatattg cagtgtttgg
     4441 caatgacatg cccggggact ggaactataa tacttttctt tcccgggaaa cagcaggtat
     4501 aaacaggcgg ataataaata cctcatccga tatggcgcag gtgaagctta ttcccggcct
     4561 tatcagcaag ttacccggat ttgaatccga aaatgcccat gaaacggctg tgatactagc
     4621 tgacgaaact ctgctgattc cttttctttc gagcctgccc gaaaatagcg gcaacataaa
     4681 tatcactatg ggatttcccc tccggcacac agcagtatat gcctttatac ggcggatcat
     4741 ggaaatgcag gtcagggcaa gaagtgaaaa cggtacaata ctcttcag
//
LOCUS       DAOSPX010000258         4740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16362_length_4740_cov_4.317396, whole genome shotgun sequence.
ACCESSION   DAOSPX010000258 DAOSPX010000000
VERSION     DAOSPX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16362_length_4740_cov_4.317396"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..881
                     /locus_tag="PLZ75_11145"
     CDS             18..881
                     /locus_tag="PLZ75_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOS72989.1"
                     /translation="MQNKTELITNPDGSIFHLNLLPGDISDKIIIVGDPGRVDMLAEL
                     FDEIRVRKENREFRTVTGTMAGSEITVISSGIGTDNIDILINELDALVNIDLKTGEPR
                     EESVSLNLIRIGTSGGLRDDVPAGSFVLTETAVGFDGLLHFYSEFDWILDTLLSDLLA
                     EYLEWPDTLPYPYAVKASEQLAGFFSPEKFIRGITISAPGFYGPQGRKLRLEPFDSEM
                     NRKLSGFTFRGKKICNYEMESSALYGLSALLGHKALTVCAVIGNRASGEFISDYKPLI
                     RELAMNVLQII"
     gene            948..1934
                     /locus_tag="PLZ75_11150"
     CDS             948..1934
                     /locus_tag="PLZ75_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72990.1"
                     /translation="MSTTGPVKETVSDEIDLRDLCRRMSQTMSDWCRKTGRAFLITVI
                     FLLKNILLLTFSILAGVGISFALKWTTKPEFISEITLRSNAVPTADMISNINQLDILI
                     REKNYSSIASALSSSPEEVVKIKRIKALWVIDKNRDNIPDFTDYRDSHNVYDSVNVRM
                     TDRFVVQVKVENSDEISKMRDRLFSYVNKNPVFIRHNEFRLKTIDELLLRINYDIEQL
                     DSLQKVKYFEETRNIKPEKGGQIIFLQDHSTQLVYDDIYTLYLRKQSLDEEKSLNNQI
                     LSVINDFYQPLRRHNGGWYYGKTVIPACLFLTLLFLIIKRNRARLKEIFSKY"
     gene            complement(1936..3243)
                     /locus_tag="PLZ75_11155"
     CDS             complement(1936..3243)
                     /locus_tag="PLZ75_11155"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOS72991.1"
                     /translation="MRNSLTFTRRNFQSEEKWIVCGIYTLSITLFLMPFPRSWSLYPL
                     ALTMCFGLVSWITDFDYYIKRFRSEILTLIPLILYFLIHALALIHDFNLNIVIERLMF
                     ILVPVLLFPLLISGQFTERIKTLLLFYITGIILIAIYQFLRASFESISIEEGFLTFQH
                     RSSPDSSRFSWTNLSKFENPTYISIKAIWAFTLLLFAQSVLKIKKTWSFLLGAVLLSF
                     IYLLAARAGFIILFLIIIYFIFYKIKRKFYRIAFVFLIPFIFFISFSIMRTNERLNYW
                     SEHVINKISSDDVDWINIEPRTRSWYSTFNIIKRKPLFGVGLNSRQILAEEHRKQGFN
                     VEADFQLNSHNQFLETQLAFGIPGSLILLWMLINPVAKRKRSWNPDLIIPFLIIVTLS
                     MLFESILVRQWGIMFFVLFYCILLIPVNSSDQPGETAIQKNPS"
     gene            complement(3277..4266)
                     /locus_tag="PLZ75_11160"
     CDS             complement(3277..4266)
                     /locus_tag="PLZ75_11160"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOS72992.1"
                     /translation="MPTIIKISDYKQLYAFANERTSHTAQTPTLGGLAVFAGFTLAVI
                     IFSHRDAAPELAYILGALITIFFIGLKDDILVIDPRKKLLGQILASMIVIWLGRFLLS
                     DFHHVFGIGKVSFMISLIFTLFVFLVIINGFNLIDGIDGLASGIGFLASAFYGTWFIL
                     AGSINYAILSLSLAGALISFFRFNVFGKKNKIFLGDTGSMIIGFIIAVLTVKFLESNL
                     TVSSKFRLGSAPAVAISLLIVPFFDTLRIVFLRLKSGKSPFRADHNHIHHILLTGGHS
                     HLSVTLMILFINILLIFLVLLFQDLGSVILTFLMLTFMIVFIFVFPVIRKVKK"
     gene            complement(4434..>4740)
                     /locus_tag="PLZ75_11165"
     CDS             complement(4434..>4740)
                     /locus_tag="PLZ75_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72993.1"
                     /translation="GGTNPSLLEAMGSGNVIVAHDNRFNREVTAGKQFYFRDAGELSD
                     CIEKIESLGKSDRDLYRDSGLELVRNKYNWSKILEKYLILLKEIRSEKEYLSQDMYC"
BASE COUNT         1560 a          926 c          954 g         1300 t
ORIGIN      
        1 gtgcatcatt taactttatg cagaacaaaa ccgaactgat cacgaatccc gacggcagca
       61 tatttcatct taaccttctg cccggggata tttccgacaa aataatcatt gtgggtgatc
      121 ccggccgggt tgacatgctg gctgaattat ttgatgaaat aagagtaagg aaggaaaacc
      181 gcgagttcag aacagtcacc ggaacgatgg ccggaagtga gatcacggtc atttcctccg
      241 gtatcggaac ggacaatatc gatattctga tcaatgaact ggatgcactt gtaaacattg
      301 acctgaagac aggcgagcca agggaagaat ccgtatccct gaacctgatc agaataggca
      361 catcgggagg actgcgggat gatgttcccg cggggtcgtt tgtcctgaca gaaacagctg
      421 tgggctttga cggcctgctg catttctatt cggaatttga ctggattctt gacacactcc
      481 tgtcggatct cctggctgaa tacctggaat ggccggatac actcccctat ccatatgctg
      541 ttaaggccag tgaacagctt gcgggttttt tcagtcctga aaaattcatc agaggtataa
      601 ctatttcagc ccctgggttt tacggtcctc agggaagaaa actgcgtctt gagccttttg
      661 acagcgaaat gaacaggaaa ctatccggat tcacatttcg cggaaagaag atctgcaatt
      721 atgagatgga aagttctgct ctttacggat tatccgccct gctggggcac aaagccctta
      781 ctgtctgtgc agttatcggc aacagggcaa gcggagaatt catttcagac tacaagccac
      841 tgatcaggga actggcaatg aacgtactgc agatcatatg atcagctgtt tggaataatt
      901 ctgaatattc ttaaatttgt tagaacgata atctaaaatt tttgcagatg tccaccacag
      961 gtcctgtaaa agaaactgtt tcagatgaaa tagatcttcg cgatttgtgc agaagaatga
     1021 gccagacaat gtcggactgg tgcaggaaaa ccggccgggc ctttctgata acagtcatat
     1081 tcctgttaaa aaatatttta cttttgacct ttagcatatt agccggcgtg ggaatatcat
     1141 tcgccctgaa atggactaca aaacctgaat ttatatcaga aataacactc cgcagcaatg
     1201 ccgtccccac cgcggatatg atttccaaca tcaatcagct tgatatcctc attcgtgaaa
     1261 aaaattacag cagtatagca tcagcattat catccagtcc tgaagaagtc gttaaaataa
     1321 agagaatcaa ggcactctgg gtcattgata aaaaccgtga caatattcct gattttactg
     1381 attaccggga ttcccacaat gtgtatgatt cagttaatgt cagaatgact gacaggtttg
     1441 ttgtccaggt gaaagttgaa aattcggatg aaatatcaaa gatgagagac agattattct
     1501 cttatgttaa taaaaatccg gtctttatca gacacaatga attcagactc aaaacaattg
     1561 acgaattact gctaagaatt aattatgata ttgaacagct tgacagtctt cagaaagtaa
     1621 aatattttga agaaaccaga aacattaaac cggaaaaagg cggacagatt atttttctgc
     1681 aggatcattc cacgcaactg gtatacgatg atatttatac tctctacctg aggaagcaat
     1741 ctcttgacga ggaaaaaagt cttaataatc aaatcctttc agtcatcaat gacttttacc
     1801 agcccttaag gcggcataac ggaggatggt actatggcaa gacagtgatt ccggcctgtc
     1861 tgtttctgac cctgcttttt ctcattatta aacggaacag ggccaggctt aaggaaattt
     1921 tcagtaaata ctgattcagg aaggattctt ttggatggca gtttcccccg gctgatcaga
     1981 tgagtttacg ggaatcagta atatgcaata gaaaagtacg aagaacataa ttccccactg
     2041 tcgtacaagg attgattcaa agagcatgct aagggtcaca atgattaaaa agggaattat
     2101 caagtccgga ttccagcttc gctttcgttt agccaccggg tttattagca tccacaataa
     2161 tatcagagat ccgggaatac caaatgcaag ctgcgtctca agaaactgat tatgagaatt
     2221 tagctgaaaa tcagcttcaa cattaaaacc ttgtttcctg tgctcttcag caagtatctg
     2281 acgggaatta agaccaactc caaacaaagg ttttcttttt ataatattga atgttgaata
     2341 ccaggatctt gtacgcggct ctatattaat ccagtcaaca tcatctgatg aaattttatt
     2401 tatcacatgt tcagaccagt aattaagtct ttcattggta cgcattatac tgaagctgat
     2461 gaaaaaaata aaaggtatca gaaaaacaaa tgcaatccgg taaaatttcc tttttatttt
     2521 atagaaaatg aaatatatga ttatcagaaa taaaataata aaccctgccc tggcggcaag
     2581 caaatagata aaagacaaga gaaccgcacc cagaagaaaa ctccaggttt tttttatctt
     2641 taaaactgat tgcgcaaaca ggagcaatgt gaaagcccat attgccttaa ttgatatata
     2701 ggtaggattc tcaaattttg acaggtttgt ccaggaaaat ctggatgaat ccggactgct
     2761 ccggtgctga aaagtcaaaa agccttcttc aatagatata ctttcaaaag acgctctgag
     2821 gaactggtat attgcaatca gaataatgcc cgtgatatag aataaaagca gtgtttttat
     2881 tctttctgta aattgtccgg agataagaag aggaaacagc aggacaggta ccaggataaa
     2941 catcagcctt tcaatgacta tattaaggtt aaagtcatgg atcagggcca gcgcgtgaat
     3001 cagaaaatac aggattaatg gaataagtgt cagaatttca gacctgaagc gttttatata
     3061 ataatcaaag tcagttatcc agcttacaag tccgaaacac attgtcaatg ccagcggata
     3121 cagggaccag ctcctgggaa atggcatcag gaaaagtgta atactgagag tataaatccc
     3181 acagactatc catttttcct ctgactggaa atttctacgg gtaaaggtta aactgtttct
     3241 cattatggag ttcctgaaat gcgcaaatga agtattttat ttttttactt ttcttataac
     3301 cgggaaaaca aatataaata ctatcatgaa ggtcagcatc aggaatgtaa gtatgacaga
     3361 accaagatcc tggaagagca aaacaagaaa aatcagcagt atgtttataa ataaaatcat
     3421 taaggttacg cttaaatgtg aatgaccgcc tgtaagcaga atatgatgga tgtgattatg
     3481 gtcggccctg aaaggtgatt tgccactttt caatctcagg aagactatcc ggagagtgtc
     3541 aaaaaacgga actatcagca gactgattgc tactgcagga gctgaaccca atctgaattt
     3601 actgctgact gtaagattgg attccaggaa tttgacggta agaactgcaa tgataaatcc
     3661 aattatcatt gaacctgtgt cgcccaggaa tattttattc tttttaccga aaacattaaa
     3721 ccggaaaaag gaaattaatg cacctgcaag cgagagggat aaaattgcgt agttaatact
     3781 tcctgcaaga ataaaccacg taccgtaaaa agccgaggcc aggaatccga ttcccgaagc
     3841 aagtccgtca atgccgtcaa tcaggttaaa accattaata atgacaagaa aaacaaaaag
     3901 tgtgaaaata aggcttatca tgaaagagac tttgccaatt ccaaaaacat gatgaaaatc
     3961 ggacaacagg aatctgccaa gccagattac tatcattgat gccagaatct gtccgaggag
     4021 ttttttcctg ggatctatta caaggatatc atccttgagg ccgataaaaa aaattgttat
     4081 cagcgctccc agaatatagg ccagttccgg tgcggcatcc cggtgggaaa agatgattac
     4141 agcaagtgta aaacccgcaa agacagccaa tccgccaagt gtaggcgttt gtgccgtatg
     4201 ggaggtacgt tcatttgcaa aggcatataa ctgcttataa tcagatattt ttattatggt
     4261 tggcacagaa acgtatgtta ttatcaaggc acttaagaat ccggcaatta atctgatact
     4321 gtccggaatg aatattatat cagacaaata attattatcc acaatagcaa aatattatga
     4381 ttcttaaata acggaaatat aaccctgaca ttaccaatac ggttttataa aaatcaacaa
     4441 tacatatcct gtgagagata ttctttttcc gatctgattt ccttaagtaa aattaaatat
     4501 ttttccagta tcttactcca gttgtattta tttctcacaa gttccagacc actatccctg
     4561 taaaggtccc tgtctgattt tccaagagat tcaatttttt ctatacaatc agaaagctca
     4621 ccggcatctc tgaaataaaa ctgcttccct gcggtaactt cccggttgaa tctgttatcg
     4681 tgtgccacta taacatttcc acttcccata gcttcaagca gggagggatt ggtcccccct
//
LOCUS       DAOSPX010000259         4740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16363_length_4740_cov_4.042903, whole genome shotgun sequence.
ACCESSION   DAOSPX010000259 DAOSPX010000000
VERSION     DAOSPX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16363_length_4740_cov_4.042903"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLZ75_11170"
     CDS             <1..555
                     /locus_tag="PLZ75_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS72994.1"
                     /translation="VLGNFFRGGAEIQAVSTIVNEDSYPLASIVRYEFPAREDMPPLS
                     LTWYDGGMRPMQIPEMESGMKMGSGGTLYIGDRGKILDDRILPLSLRESYTPPAPYIP
                     SSPGHEEEWINACKGGAAAGSDFEWAGPLTETVLLGNIALRKELKEIMSGESLRFDPE
                     RFCFPDLPEADKFLHTTYREGWSL"
     gene            592..1545
                     /locus_tag="PLZ75_11175"
     CDS             592..1545
                     /locus_tag="PLZ75_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS72995.1"
                     /translation="MKQNRREFITKIAAAAAAIPFAKIHRPAPGNSSAERFPVRLFSK
                     PLDEYDFSFMCECLKRSGIGGFDLTVRPGGKVIPERVENDLPRLAEEAEKYGLVLDMM
                     VTSILYPDEEFTEKILKTASMAGVSHYRLGWAEYEPGVNIMKALQGYKYAMKGIAGLN
                     EKYNIRGGYQNHAGTWIGGPVWDLYELLKDLPPEFIGCQYDVRHAMVEGTNSWILGLK
                     LLLPMINSLAVKDYTWQTVKGRPMAVTVPLGEGMVNWDLFFGTLRDFNVSAPVTLHVE
                     YPWFSKAEEGYSLIRKQDIIVKKLKKDMDFLNGYLSKYNLV"
     gene            1588..2949
                     /locus_tag="PLZ75_11180"
     CDS             1588..2949
                     /locus_tag="PLZ75_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOS72996.1"
                     /translation="MAAGAAATAAPLEMVMASCSPAPEKRISISGVDSNFEREPLIRP
                     FGFKGGYLTELWQTVSRLESSSGVRTTGLCTQSILWSDESVFASHSEAGGNALMYAIT
                     ERALQMVNGQSFTLPYVLLDEILPELYSYGCGITANPRLRKTFVLNSLVGLDNALWLL
                     YAGENKITTFDGLIPEACKPAFSFRHDRVAGIPLMAYTVPVEEIKATAEEGYFVMKIK
                     IGQPGTQEEMLEKDKERLSAIHKAIGNVETPWTENGRLPYYFDANGRYEKRETLMMFL
                     EHAKHIGAFDQIVIIEEPFPEEMETDVRDIPVRLAADESAHTAEDAIRRIEMGYGAIA
                     LKAIAKTLSMTMKIAQAAYERNIPCFCADLTVNPVLVEWNRNVAARLSPFPGMGSVGL
                     MESNGHQNYLNWDSMLKYHPRSDAPWIGIRKGFYELDREYYRTGGGIFDPIPHYESMF
                     AES"
     gene            complement(3016..3861)
                     /locus_tag="PLZ75_11185"
     CDS             complement(3016..3861)
                     /locus_tag="PLZ75_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOS72997.1"
                     /translation="MLKITLTGAGGKMGLRLTRNLMRSKYKISYLEVNSVGIHKLKEM
                     GITVCRQEQCLPEADIVILAVPDTSLERLSEEIIPVMKTGSMIILLDPAAALAGKLYY
                     REDISYFIAHPAHPSVFNWEPERDAMNDHFGGVAAKQSIVCTLVRGSEKDFKRGEELA
                     RVFYAPVERSHRLSLEQMGLLKPALVTTLASACMFTIRQGLDEVIDKGVPAEAARDFL
                     LGHLRMQMAVLFDELPGAVFSDSTNKALQIGLEEIISEDWRDIFDSENIREQIKIITS
                     PAPIY"
     gene            4189..>4740
                     /locus_tag="PLZ75_11190"
     CDS             4189..>4740
                     /locus_tag="PLZ75_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS72998.1"
                     /translation="MKLGFVSAILPELGFEELIDYAALLKYECVEICCWPVGKAERRY
                     AGVTHIDVDTLDDRKKEHISQYVRQKNITISALGYYPNPLDPDREKGSFYIQHIRKLI
                     EASSKLGINRINTFIGRDKNRTVEENFESFRLIWPDIIRFATDRGVKVGIENCPMIFT
                     RDEWPGGNNLATTPVIWRRMFEEI"
BASE COUNT         1300 a         1011 c         1217 g         1212 t
ORIGIN      
        1 gtgctcggga atttcttcag gggcggtgct gaaatccagg cggtttcaac aattgtgaat
       61 gaggacagct atccccttgc ttccattgta agatatgaat tcccggcccg tgaggacatg
      121 cctcccctga gcctgacatg gtatgacggc ggcatgagac ccatgcagat ccctgaaatg
      181 gagagtggca tgaagatggg atctggcggg acactctaca taggcgacag ggggaagata
      241 ctcgatgaca ggatccttcc cctttcactg agagaatcat atacgccacc ggctccgtat
      301 atcccgtcat ctccgggaca tgaggaagaa tggataaatg cctgcaaggg aggagcagcg
      361 gcaggttccg attttgaatg ggccggcccc ctgactgaaa cggtgctgct tggcaatata
      421 gccctcagga aggaactgaa agagatcatg agcggggaat ctctcaggtt tgatccggaa
      481 agattctgtt tccccgacct ccctgaagca gataagtttc ttcatacgac ttaccgtgaa
      541 ggctggtcac tttgaaatta ttttcgttct ggtttttaga tttgaaaaaa tatgaaacaa
      601 aacagaagag aattcattac aaaaattgca gctgcagctg cagcaatacc tttcgcaaaa
      661 atacaccggc cggccccggg gaattcaagt gccgaaagat ttccggtaag gttattttca
      721 aaaccccttg atgaatatga tttcagtttt atgtgcgaat gcctgaaacg gtcgggtata
      781 ggaggatttg atctgaccgt acggcccggg ggtaaggtta ttccggagag ggtagagaat
      841 gatcttccgc gccttgctga agaagctgaa aaatacggtc ttgtcctgga tatgatggtt
      901 acttcgatac tctatcccga tgaggagttc acggaaaaga ttctcaaaac agcttccatg
      961 gcaggagtaa gccattaccg gctcggatgg gcagaatatg aacccggggt gaatatcatg
     1021 aaggcattgc aggggtataa atatgcaatg aagggtattg ccggcctgaa tgagaaatac
     1081 aatatccggg gcggctatca gaatcatgcc ggtacctgga taggcggccc ggtatgggat
     1141 ctctatgaac ttcttaaaga tctgcctccg gaatttatag gttgtcagta tgatgtaagg
     1201 catgcaatgg ttgaaggaac aaactcgtgg atactcggac tgaagctttt gctgccgatg
     1261 atcaattccc tcgcagtaaa agattataca tggcagaccg taaaaggcag accaatggcg
     1321 gttaccgtcc ctctcggcga gggaatggtg aactgggatc ttttcttcgg aacattgagg
     1381 gatttcaatg tatcggcacc ggttaccctt catgttgaat acccctggtt cagcaaagcc
     1441 gaagaaggtt attcactgat caggaaacag gatatcatcg tgaaaaagct aaaaaaggat
     1501 atggattttc tgaacgggta tctttctaaa tataatttgg tttaaaatct tatgactgac
     1561 cggagggaat ttataaaaaa aagcctcatg gctgcaggtg ctgccgcaac tgcagcaccc
     1621 ctggaaatgg tgatggcttc ctgttcccct gccccggaga aaaggataag catttcagga
     1681 gtggattcta attttgagag agaaccatta atccgtccat tcggctttaa gggcggctat
     1741 ctgacggaat tatggcagac agtatcccgt cttgagagca gttcgggtgt ccggacaaca
     1801 gggctttgca cacaaagcat cctgtggtcg gatgaatcag tgtttgcatc gcattcggag
     1861 gcaggcggga atgcgctgat gtatgcaata acggaaagag ccctgcagat ggtcaatggt
     1921 cagtcattta cccttccgta cgttctcctt gatgaaatac tgcctgagct gtattcctac
     1981 ggatgcggga ttactgccaa cccccggctg aggaaaacat tcgtgctgaa ttcgcttgta
     2041 gggcttgaca atgccctgtg gctgctatat gccggggaaa ataaaatcac aactttcgac
     2101 gggctgatac cggaggcctg caaaccggct ttttccttca ggcacgacag ggtggcagga
     2161 attcccctga tggcttatac cgtgccggtt gaagaaataa aagcaacggc tgaagaagga
     2221 tatttcgtca tgaagatcaa gatcggacag ccggggaccc aggaagaaat gctggaaaaa
     2281 gacaaggaga ggcttagcgc catccacaaa gccattggaa atgtagagac cccctggaca
     2341 gagaatggca gactgcctta ttatttcgat gccaacgggc gttatgagaa gagggaaaca
     2401 ctgatgatgt tcctggagca tgcgaaacac atcggggcct ttgaccagat agtcattatt
     2461 gaggaacctt ttcccgaaga gatggaaact gatgtcaggg atattcccgt aaggcttgct
     2521 gcggatgaga gtgcacacac tgctgaagat gccataagga ggattgagat gggctatggt
     2581 gccattgcat tgaaggctat tgccaagacg ctcagtatga ccatgaaaat tgcgcaggca
     2641 gcatatgaaa ggaatatccc ctgtttttgt gctgatttaa ctgtaaatcc tgtgcttgtg
     2701 gaatggaaca ggaatgtggc ggccaggctg tctccatttc ccggcatggg cagtgtcgga
     2761 cttatggaaa gcaatggtca tcagaactat ctgaactggg acagcatgct taagtatcat
     2821 ccgcgcagtg acgcaccctg gattggaata aggaagggat tttatgaact tgacagggaa
     2881 tactaccgta caggaggagg aatttttgat cccatacctc actatgagtc aatgtttgct
     2941 gaaagctgaa aaacaagttc agtaagtaaa ttcatattcg cagctccggg gaagaggaaa
     3001 gccggcgcag ggtattcagt atatgggtgc cggtgatgta atgattttaa tctgttccct
     3061 tatgttttca gagtcaaaga tatcccgcca gtcttcactg ataatttctt ccaggcctat
     3121 ctgaagtgct ttatttgtgg aatctgaaaa gaccgctccg ggaagttcat caaacagtac
     3181 cgccatttgc atccgcaggt ggcccaggag gaaatccctg gctgcctctg ccggtacacc
     3241 cttatcaatt acttcatcaa gtccctggcg tatcgtgaac atgcaggctg aagcaagagt
     3301 tgtgaccagt gccggtttaa ggagtcccat ctgttccagg ctcagcctgt gcgatctttc
     3361 aaccggtgca tagaataccc gcgctagttc ttcccctctt ttgaaatcct tttcgcttcc
     3421 cctgaccagt gtacatacaa tggactgctt tgccgccact ccgccaaaat gatcattcat
     3481 ggcatccctt tcaggctccc agttgaatac tgagggatgg gcaggatgag caatgaaata
     3541 ggaaatatcc tcacggtagt atagtttacc ggccagcgca gctgccggat caagcagtat
     3601 aatcattgat ccggtcttca tcaccgggat tatctcttca gagagccgct ccagggaagt
     3661 gtcaggcacg gcaagtataa caatgtcagc ctcgggcaga cattgttcct gacggcacac
     3721 cgtgataccc atttccttca gtttatggat tccgacactg tttacttcca gataggagat
     3781 tttgtatttg gatctcatca ggttacgggt aagccgtaat cccattttgc ctccggctcc
     3841 tgttaatgtt attttcaaca tgtttcaata ttttcaatat gtttcaatat gtcggtgaaa
     3901 atagtttggt tcattcattt ctgcagcggc ggtaccgcgg atcattcaca gccgggcatc
     3961 atgccggcgc tgaatcgttc acggtcccgg tgtgaaaaca ataacgcaaa aaaagccaaa
     4021 attatcagtg tttttaagat tttcacgaaa tttatttaaa atgtttcaat tataaatact
     4081 tatgcgtttt gcctgtcagt tttactgatt tactggctgg agaagaaaac agaaccgggc
     4141 ctgatttctt ataattatgc atatatttaa cagaaaaagg aggttgtcat gaaactggga
     4201 tttgtaagtg caattcttcc cgaactgggt ttcgaggaac tcattgatta tgcagcgtta
     4261 ctgaaatatg aatgtgttga gatatgctgc tggcccgtcg gaaaagctga aagaagatat
     4321 gcgggagtga ctcatattga tgtggatacg ctcgatgata ggaaaaagga gcacatcagt
     4381 cagtatgtca gacagaaaaa tatcacgata tctgcactgg gttactatcc gaatccgctt
     4441 gatccggaca gggaaaaggg gtctttttac attcagcata tcaggaagct gattgaggct
     4501 tcctcgaaac tgggaataaa ccgcatcaac actttcatcg gaagggacaa gaacaggact
     4561 gttgaagaga actttgaatc attcaggctg atctggcccg atataatcag atttgcaaca
     4621 gacaggggtg ttaaggtcgg aatagagaac tgtccgatga tttttacgag ggatgaatgg
     4681 cccggaggga acaatctcgc cactactccg gttatctggc gcaggatgtt tgaagagata
//
LOCUS       DAOSPX010000260         4714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16464_length_4714_cov_3.779566, whole genome shotgun sequence.
ACCESSION   DAOSPX010000260 DAOSPX010000000
VERSION     DAOSPX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16464_length_4714_cov_3.779566"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..971
                     /locus_tag="PLZ75_11195"
     CDS             15..971
                     /locus_tag="PLZ75_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS72999.1"
                     /translation="MLVTGGAGFIGSNLIESFLSCGNQVSCLDNFSTGKRENLKEYMD
                     HPDFRLLEGDIRNYNDCLEAVSDTDIVFHQAALGSVPRSIKDPVTSTDVNIGGFVKML
                     FASKEAGVKRFIYAASSSTYGDHPGLPKVEDRTGKPLSPYGITKYVDELFAANFASTY
                     NIEVIGLRYFNVFGRRQDPHGAYAAVIPLFVKKLMNHEAPVVNGNGSFSRDFTYIDNV
                     IQANHLAALADRQEAINQVYNVAHGERTDLNTLFELIRDIAGRYDRRVLEIKPVYGPE
                     RAGDIPHSLASIEKAKNLLGYSPTHNVKKGLEEALEWYWENL"
     gene            1015..2517
                     /locus_tag="PLZ75_11200"
     CDS             1015..2517
                     /locus_tag="PLZ75_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOS73000.1"
                     /translation="MPASSRNIIALNVSTSVIQVIITGLVYFFIYRILVLQLGVELLG
                     VWSLTIAVASVANLSNLGFSTAIIKYVAEYDAREESEKISKLLYTSIISVSVLFIIAG
                     ILVCSLAFFFITRLVEIKYFRLAMTILPISLCGLFFVSLANILTSVLEGFQKNYIRNI
                     TCGTTSVLFLVLAIILIPRSGLVGLAIAQAIQALALFIVSCFLLSKILKGFLIKEIKW
                     DRQAFKQLLNYGYRMQLISVGQILTEPVTKILISRFHGMNTLGFYEMASRVIGQLRQI
                     IAGMDQITMPIVSHFQQTQKITVQYIYEKGLSFVLFIVFPLFAGIILFAPYLSKIWIG
                     SVEKVFMNCMYILAAGMLVNVLNVQAYFNSLGEGRLNGILIMILFILLFNTLTGIVAG
                     YFIPVYGVVIANAVASATGSVWFINNYHRTTGINLKKILKKPDLAIMAGSLLFAVFAG
                     FVLLKISRFYDYSLISLFGMMSIYLIYFMILSRPNDNFKLLNIFQYFRNR"
     gene            2750..3877
                     /locus_tag="PLZ75_11205"
     CDS             2750..3877
                     /locus_tag="PLZ75_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73001.1"
                     /translation="MKITIKPVIVLLLVALPLLWALLMSGQSDLYHFSQGFFYLSIPL
                     IMIITGYQLSKIFSLHQFFISIVIIGIITAILFIILTVISAGFAAFLSPYTEARFVVA
                     SGSSVCILSLITAAYSEYFGIKIFKKKKGKYLAVIINLIAIYLFASRTYWVFLFVFVI
                     LFSLKTLRRNKLLFAGFMLIAVLLISLSVIGSKEGLTFQNSILYKLINSFKEVKVRDI
                     TDYEYINLYFRGYEAYRSWVTFSESSLLHKVLGGGYGKLVTLDAEVLLDGKYWNAVPW
                     VHNGFFFILVKEGLLGIVFALLFFFYLSRESIKGYRKDTCQRQYARLVLLGCTITLFV
                     ANYVVCGLFSHEMAILLISIGFLLSGLKQNGTEHEITSYKN"
     gene            3852..>4714
                     /locus_tag="PLZ75_11210"
     CDS             3852..>4714
                     /locus_tag="PLZ75_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73002.1"
                     /translation="MKLRLIRTKNPDKQHVSVADVYDFETTAMELFPFIICNESGYFR
                     KAVRPSVAGYINKLLPKTKAPAFCISMGINGISRSFPHLFLSSGNILYLFDAWPGSYE
                     LIGKTIKAYNIGLLLTSSRESAAALQKMYSDICVLWCPEGIRNTVYRAYDYSQKDIDV
                     IQIGRKYDLWHDRVVGKLKHSGISYVYEKVKGEVIFRHRTDFFEGLGRSRISVCFPRS
                     VTHTEKSGGLTTMTNRYLQSAASKCLILGIIPGEMKDLFGYDPGITADMNDPAGQIKE
                     ILDNFKDYIPL"
BASE COUNT         1323 a          913 c         1019 g         1459 t
ORIGIN      
        1 taaagaataa aaaaatactt gtaaccggcg gcgccggctt tataggctca aatcttatcg
       61 agtcattttt gtcctgcggc aaccaggtaa gctgtctaga taatttctca accggaaagc
      121 gtgaaaacct gaaggaatat atggatcatc cggacttcag gctgcttgaa ggtgatatca
      181 ggaattacaa tgactgcctg gaagccgtgt cggatacgga tattgtgttc caccaggctg
      241 ccctgggctc tgttccgcgg tcaataaaag atcctgtaac atcaacagat gtaaatatcg
      301 ggggatttgt gaaaatgctt tttgcttcaa aggaagcagg tgtgaagaga tttatttatg
      361 ctgcaagctc ctccacatac ggggatcatc ccggactgcc caaggtggag gacaggacag
      421 gaaagcccct gtctccctac ggaattacaa aatatgttga cgaacttttt gccgcgaatt
      481 ttgcttctac atacaatatt gaagttatcg gactgcgtta tttcaatgtt ttcggcagac
      541 gccaggatcc tcacggagcc tatgccgcag tcataccgct tttcgtaaaa aagctcatga
      601 accatgaagc tccggtcgtt aacggcaacg gcagcttttc gcgtgatttc acctatatag
      661 acaatgtcat tcaggcaaat catcttgctg cacttgcaga caggcaggaa gcaataaacc
      721 aggtttacaa tgttgcacac ggggagagga ccgatctgaa cacactcttt gaactgatac
      781 gtgatattgc agggaggtat gacaggcggg tacttgaaat aaaaccagtg tatggtccgg
      841 agagggcagg ggatatacct cattcactcg catcaattga aaaggcaaaa aatcttctgg
      901 gctattcccc cacacataac gtgaaaaaag gccttgaaga agccctggaa tggtattggg
      961 agaatttata gataaaagaa ttacctgaat ctgaataatt aactctttat cccggtgccg
     1021 gcaagttcaa ggaacataat agcccttaat gtaagtactt cagttattca ggttataatt
     1081 acaggattag tctatttttt tatctacagg attctggtac ttcagctggg ggtcgaactg
     1141 cttggagtgt ggtctcttac aatagctgtc gcttcagttg ccaacctttc aaatcttgga
     1201 ttttccacgg caattatcaa atatgtagct gaatatgatg caagggaaga atccgagaaa
     1261 atcagcaaat tactctatac ctcaattata tctgtatccg ttttattcat aattgcggga
     1321 atactggtct gttctctggc tttctttttt ataaccaggc tggttgaaat aaaatatttc
     1381 agactcgcaa tgacaatact gccaatatct ctttgcggac ttttctttgt ttcgctggca
     1441 aatatcctta catcggtact ggaaggtttt cagaagaatt atatcaggaa tatcacctgc
     1501 ggaacaacat ccgtattgtt tctggtcctg gcaattatcc tgattccccg ttccggcctg
     1561 gttggactgg cgattgccca ggccattcag gcccttgctt tatttattgt ttcctgtttt
     1621 cttctatcaa aaatactgaa aggctttctg ataaaggaaa taaaatggga caggcaggcc
     1681 tttaagcaat tgttgaatta tggatatagg atgcagctga tatcagtagg gcagatcctt
     1741 acagagccgg ttaccaagat acttataagc cggttccacg gaatgaatac cctgggcttc
     1801 tatgagatgg ctagccgtgt aattggtcag ctgaggcaga taattgccgg aatggatcag
     1861 atcacaatgc caatagtatc gcattttcag caaactcaaa agattacggt tcagtatatt
     1921 tatgagaaag gcctttcatt tgtgttgttc atcgtgttcc cgctgtttgc gggaattatt
     1981 ttatttgcac cttatctgtc aaagatctgg ataggcagtg tagaaaaggt attcatgaat
     2041 tgtatgtaca ttcttgcagc cggtatgctc gtcaatgtgc tgaatgtgca ggcttatttc
     2101 aattccctgg gagagggccg tctgaatggc atcctgatta tgatactgtt tattctgctc
     2161 tttaatactt taaccggaat agtggcggga tattttatcc ctgtttacgg tgtcgtgatc
     2221 gcaaatgcgg tggcaagtgc gacaggatca gtatggttta tcaataacta tcacaggaca
     2281 accggtatta acctgaagaa aatcctgaaa aaacctgatc tcgcaattat ggcaggcagt
     2341 ctgttgtttg cagtttttgc aggatttgtt ttacttaaaa tcagcagatt ctatgactac
     2401 agtttaatat ccctgtttgg catgatgagc atttacctga tctatttcat gattctgtcc
     2461 cgtccgaacg ataattttaa actgctgaat attttccagt atttcaggaa ccggtaatat
     2521 atttataata ttggatcagg aacaagtaaa tattacagat ttttgatatt tcggtaaggg
     2581 gataatgtat aaaattaagt ctgttataag agtcccggga aggtctgcac tgacaagaac
     2641 aatactgatg tctttgcttt tctccttctt aaccctgtat ttttttatat cacataagat
     2701 tatatggctc ttgtatttgt cttttctcgt cataattgct gcggccagga tgaaaatcac
     2761 aatcaaacct gtgattgttc tcctgctcgt tgcgctgccc ctgctatggg cactgctgat
     2821 gtccggccag agtgacctct atcattttag ccagggtttt ttttatctct ctattcctct
     2881 gataatgatc attactggtt atcagttgtc aaaaatattt tcacttcacc agtttttcat
     2941 ctccattgtt ataataggaa taataactgc aatccttttt ataattctga ctgttatcag
     3001 tgcaggcttt gccgctttcc tttcacccta tacagaagca agatttgttg tcgcaagcgg
     3061 ttcttccgta tgtattttaa gtctgatcac agccgcatat tcggaatatt tcggaataaa
     3121 aatttttaaa aaaaagaaag gaaaatatct ggccgtaatt ataaatctca tagcaatcta
     3181 cctgtttgca tcaaggacct attgggtatt tctttttgtt ttcgtcatcc tttttagttt
     3241 aaagacactg cgccgcaaca agctgctgtt tgcaggattt atgctgatag cagtactgct
     3301 gatttctctc tcagtaattg gttcaaagga aggactcaca tttcagaaca gtatcctcta
     3361 caagctgatt aattcattta aggaggtaaa ggttagagat ataactgatt acgaatatat
     3421 taacttgtat ttcagaggct atgaggctta caggagctgg gttacatttt ctgaaagcag
     3481 tcttctgcat aaagtcctgg ggggaggata cgggaagctt gtaactcttg atgcagaggt
     3541 tttgctggat ggaaaatact ggaacgcagt accatgggtg cataacggat tctttttcat
     3601 acttgtgaag gaaggattgc ttgggattgt atttgccctt cttttctttt tttacctgag
     3661 cagggaaagt atcaaaggat acaggaaaga tacctgtcag agacaatatg cccggcttgt
     3721 tttactgggt tgcacgatta ccttgtttgt agccaattac gttgtctgtg gactgttcag
     3781 tcatgaaatg gcaattcttt taatatcaat cggatttctt ttgtcaggcc tgaaacaaaa
     3841 cggaacagaa catgaaatta cgtcttataa gaactaaaaa tccggataag cagcatgtat
     3901 ccgtggccga tgtttatgat tttgaaacga cggcaatgga attattccct tttataatct
     3961 gtaatgaatc cggatatttc cgaaaagctg tcagaccctc tgttgccggt tatatcaata
     4021 aattattacc aaagaccaaa gctcctgcat tttgcatatc tatgggaata aatggcatca
     4081 gcagaagttt tccgcatctc tttctttcat ccgggaacat tctttatctt tttgatgcct
     4141 ggcccggatc ttatgaatta atcggaaaaa caataaaagc ctataatatt ggactgctgc
     4201 tgacatcctc cagggagtcc gccgcggccc tgcagaaaat gtacagcgat atttgtgtgt
     4261 tatggtgccc ggaagggatc agaaacactg tttacagggc ttacgactat tctcagaaag
     4321 atatcgatgt aatacagata ggaaggaaat atgatctctg gcatgacagg gttgtgggga
     4381 aactgaaaca ttccggcatc agttatgtgt atgaaaaggt aaagggagaa gttattttca
     4441 ggcacagaac cgattttttt gaaggattgg gacgaagcag gatttccgtc tgtttccccc
     4501 ggtcagttac tcatactgaa aaaagcggag gtctgaccac aatgacaaac agatatcttc
     4561 agtcagctgc atccaaatgt cttattctcg gaataatccc cggcgagatg aaggatctgt
     4621 ttggttacga tcccgggatt acagcggaca tgaatgatcc ggccggacag attaaagaga
     4681 ttttagataa ctttaaggat tatattcccc tgat
//
LOCUS       DAOSPX010000261         4709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16491_length_4709_cov_3.930382, whole genome shotgun sequence.
ACCESSION   DAOSPX010000261 DAOSPX010000000
VERSION     DAOSPX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16491_length_4709_cov_3.930382"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..628
                     /locus_tag="PLZ75_11215"
                     /pseudo
     CDS             209..628
                     /locus_tag="PLZ75_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819141.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
     gene            1148..1276
                     /locus_tag="PLZ75_11220"
     CDS             1148..1276
                     /locus_tag="PLZ75_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOS73003.1"
                     /translation="MHSISQKYGVKLNKLYEYNRMARGSEAEAGQVIWLRKAKPAR"
     gene            complement(1242..2234)
                     /gene="fmt"
                     /locus_tag="PLZ75_11225"
     CDS             complement(1242..2234)
                     /gene="fmt"
                     /locus_tag="PLZ75_11225"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOS73004.1"
                     /translation="MKKLRIVFMGTPEFAVATLGSLLDNGYDVVSVVTAADKPSGRGR
                     KTSRSPVSIFAGSHSLPVLQPENLKDPEFISALKKLEADVFIVVAFRMLPEAVWRIPP
                     CGTINLHASLLPHYRGAAPINHVLINGETVTGVTTFLIDDNIDTGKILLREEIPVDPS
                     ENAGDLHDKLMKRGAALVLRTLAAISAGQISPQPQSAFLMPGEIPRTAPKIFPENCII
                     NWQNECHAIHNFIRGLSPSPGARSFLKQDGETLSFKVFESRPEIAVHTLVPGSVISDR
                     KNYIKIACKNGYVNIDSLQLAGRKRMNISEFLKGFRLADHPVAVTWQALPSSAR"
     gene            complement(2261..3499)
                     /locus_tag="PLZ75_11230"
     CDS             complement(2261..3499)
                     /locus_tag="PLZ75_11230"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOS73005.1"
                     /translation="MNNIKLNTIEEAIEDIGRGKLVIVVDDEDRENEGDFICAAELIT
                     PGIVNFMTKHGRGLICVALPEERCEELELDLMVGNNTSLHETQFTVSVDLISPETTTG
                     VSAKDRALTIKALVDPGTRPSDLGRPGHVFPLKAKAKGVIRRAGHTEAAVDLTVLAGL
                     NPGGVLVEIMNDDGSMARLNDLVGLGEKFGIKIVSISDLIKYKLERDTIIEVGERVKL
                     PTDRGEFDFIPFRQISNGLEHGALVKGSWTKDDTVMVRVHSSCFTGDIFGSLRCDCGP
                     QLHRAMEMVEKEGKGVVIYLSQEGRGIGLFNKIKAYRLQDEGMDTVQANLKLGFAEDE
                     RDYGVGASIMRALGLGKIKLISNNPVKRAGLEGYGIKIVETIPLIIESNPHNEFYLQT
                     KEKKMGHNLTCYKHLAKDRP"
     gene            complement(3492..>4709)
                     /locus_tag="PLZ75_11235"
     CDS             complement(3492..>4709)
                     /locus_tag="PLZ75_11235"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOS73006.1"
                     /translation="SLMRIMRPLMFLIGILVIISFLFSNYVLPYSNRQARMLLYDIRR
                     KRPDINLQAGSFNNDIDGFSIKVTAKDPVTNRLDGIIIYDHRDKKGNISVTTADSGYM
                     KITPDETAMIMTLYNGKSFTEIEEKNVAPDQRKYPSRKDEFREQTIVISLSGFDLQRS
                     SDDLFRSTSWMLNLSQLSFYIDSMNNEYNIKYYQQYGEFRDSKIYTGKNVMYDVDGED
                     QEKDSVFFNPGQLYSGLRSHDKKIVVARALENAKEAANFLSMTTETMKMKIRFMKRYE
                     VDWHKKLVLPFGCLVFFFIGAPLGAIVRKGGLGTPTVISILFFVIWYVISMSAEKFVE
                     ENLISSFAGMWTSSFILLPIGIFLTYKASHDSVIMNVDTYLQFFRNIKNFLFRIIFQG
                     KKPKYDIINITDE"
BASE COUNT         1170 a         1113 c         1104 g         1322 t
ORIGIN      
        1 ttccagctta tagtaagcgc ggattgatgt tcacatcagc ctgcatgaaa ttaattcact
       61 ttttactatt tttaccgcaa acggaagatc aatgcacaaa agcccttact gtatcttttt
      121 tctgttcctc cttggcggtc ttgtttcgtg cggggttttc aggccggcct cagtatcctc
      181 cccggcccct ctcacaacat ctgcagcaga atatatccag cggtacaagg atttggctgt
      241 cagcgaaatg gctcgatcgg gagttcccgc cagcattact ctcgcccagg gaatggtgga
      301 atccgattac gggcgcagca ctctcgcccg cacagcaaat aaccatttcg ggataaagtg
      361 tcataatgac tggaggggtc cgtctgtgcg gcatcatgat gacagacaca atgagtgctt
      421 caggaaatac agcactgcag aggactcttt ttacgatcat tctgattttc tgcgaagcag
      481 atcacgctac agttttctct ttgatcttcc ggttaccgac tacaaaagct gggcaagggg
      541 gctgaagaag gcaggatatg ccaccaatcc ccattatgca gagatgctga tcaggaagat
      601 agaggagtac aatcttcatt tatatgatca tatgctgccg gttgccggag cagggaccgg
      661 agcagggtac ggaacatctt cagggacagc atccggaaca ggctcaggaa gaacctccgg
      721 agcagcatcc ggaacagcat ccggaacagc atccggaaca gcatccggaa cagccaccgg
      781 aacaacgtcc ggaacaattt caggaacggc ctcccggtca gtggcttctg aagaaaccag
      841 ggtcagggaa aaagagatct tgtctgacgt gaaaacaagc cggccgctga taaatgacaa
      901 tttcacggta aatattcatg atcagagggt aattgagaat aatggttcaa aatatataat
      961 tgtaagggat gaggagagca tggaaatgat catggaggag tttgatgttt tcaggtggga
     1021 gattctgagg tttaatgatc tggaagatga ttacgtgcca cggccgggac agattctgta
     1081 tattcagccc aagcgcgcca gggcagaggc cggaaaggag aaatatactg cagctgcagg
     1141 tgatacaatg cattccattt cccagaaata cggtgtaaaa cttaacaagc tgtatgaata
     1201 caacaggatg gccaggggga gtgaagcaga ggcaggtcag gttatctggc tgaggaaggc
     1261 aaagcctgcc aggtgactgc caccgggtga tccgctaatc tgaatccttt cagaaattcg
     1321 ctgatgttca tcctttttct tcctgccagc tggagggagt caatatttac ataaccattc
     1381 ttacaggcta tttttatata atttttccgg tcggatatta cagagcccgg aaccagggtg
     1441 tggactgcaa tttccggccg gctttcaaat accttgaatg aaagtgtttc tccgtcctgt
     1501 ttaaggaatg accgtgctcc cggcgaggga gaaaggcctc ttatgaaatt atggattgca
     1561 tggcattcat tctgccagtt tataatacag ttttcaggga atattttggg tgctgtcctt
     1621 ggtatctctc cgggcataag aaatgccgac tgcggctgag gactgatctg ccctgccgaa
     1681 attgctgcca atgttctcag aacaagggcg gcccctcttt tcatcagttt gtcatgcagg
     1741 tcaccggcat tctcggacgg atcaacgggt atttcctccc tgagcaggat tttgccggta
     1801 tcaatattgt catcaatgag aaaggttgtt actcctgtta ctgtttcccc gttgatcaga
     1861 acatggttga tcggggctgc tcccctgtaa tggggcagaa gggaggcgtg aagattgatt
     1921 gttccgcagg ggggaattct ccatacggct tccggaagca tacggaatgc aacaacgatg
     1981 aatacatcgg cctcaagctt tttcagggca ctgataaatt caggatcttt caggttttcc
     2041 ggctggagaa cgggcaggga gtgtgatccg gcaaagatac tgaccggtga cctgcttgtt
     2101 tttcttcccc tgcctgacgg cttatcagct gcggtgacaa ccgaaacaac atcatagccg
     2161 ttatcaagca gtgagcccag tgttgccacg gcaaattcag gcgttcccat aaatactatc
     2221 ctgagttttt tcattgcggt cacggctgta tggatgaact tcagggcctg tcctttgcca
     2281 gatgtttgta gcaggtcagg ttatgcccca tctttttctc cttggtctga aggtagaact
     2341 cgttatgtgg atttgattcg atgataagag ggattgtttc aactattttt attccatagc
     2401 cttctagacc cgctctttta accggattgt tggaaatcag cttgattttt cccagcccta
     2461 gtgcccgcat gatgctggcg ccaacgccgt agtcccgttc atcttccgcg aatcccagct
     2521 tcagatttgc ctggacggta tccattcctt catcctgaag cctgtatgcc tttattttat
     2581 tgaaaaggcc gattcccctg ccttcctgac tcaggtatat cactaccccc ttgccctctt
     2641 tttcgaccat ttccatggcc ctgtgaagct gcggaccgca gtcgcagcgc agcgacccga
     2701 aaatatcacc tgtaaagcag gaagaatgca cccttaccat tacagtatca tctttcgtcc
     2761 agcttccctt taccagcgcc ccgtgttcca ggccgttgct tatctgcctg aatggtatga
     2821 aatcaaattc ccccctgtcg gtgggcagtt tgaccctctc accgacctca atgatagtgt
     2881 ccctttcaag tttgtattta ataagatcgc tgatggaaac tatttttatt ccgaattttt
     2941 ctcccagtcc gacgagatcg ttgagtcttg ccatggatcc gtcgtcattc attatttcca
     3001 ccagaacccc gccgggatta agtccggcca gcacagtcag gtccaccgca gcctctgtgt
     3061 ggccggctct tctgattaca cccttggctt tggcctttaa gggaaagaca tgtcccggcc
     3121 gtcccaggtc ggaaggtctg gttccgggat ccaccagggc ttttatggta agggcccggt
     3181 cttttgccga gacccccgtg gtggtttccg gggatatcag gtcaaccgaa acggtaaact
     3241 gggtttcatg aagtgatgtg ttgttgccta ccatcagatc aagttcaagt tcctcacatc
     3301 tttcttccgg aagagccaca caaatcaatc cccttccgtg tttggtcatg aaattgacaa
     3361 ttcccggagt tataagctca gcggcacaga taaagtcgcc ttcattctcc ctgtcctcgt
     3421 catccactac gatgacaagc tttcccctgc ctatatcctc aattgcttct tcaatggtat
     3481 taagtttaat attattcatc ggttatattt attatatcat atttaggttt tttcccctgg
     3541 aaaattattc tgaaaagaaa attttttatg ttcctgaaaa actgaagata ggtatctaca
     3601 ttcataatta cggaatcatg tgaagctttg taggtaagga agataccgat gggcagcagg
     3661 ataaatgacg atgtccacat tcctgcaaag ctgcttatca ggttttcctc cacaaatttt
     3721 tctgccgaca tggatatcac ataccagatc acaaagaaaa gaattgaaat caccgtgggt
     3781 gttccgaggc ccccctttct gacaattgct cccagcggcg ccccgataaa aaagaaaaca
     3841 agacagccga atggaagaac aagcttttta tgccagtcga cctcatatct tttcatgaat
     3901 cttattttca ttttcattgt ttcggttgtc attgagagaa aattggcagc ttcctttgca
     3961 ttttcaagag cccgggcaac cacgatcttt ttgtcatggg acctgagacc ggaatagagc
     4021 tgccccggat taaagaagac ggaatctttc tcctgatcct caccgtccac gtcatacatt
     4081 acgttctttc cggtataaat ctttgaatcc ctgaattccc cgtactgctg atagtatttt
     4141 atgttatact cgttgttcat ggaatcgata tagaacgaga gctgggagag gttcagcatc
     4201 catgaagttg atctgaaaag gtcgtcactg ctgcgttgca ggtcaaatcc ggaaagggag
     4261 attactatgg tttgttccct gaattcatcc ttccttgaag gatatttcct ctgatccgga
     4321 gcaacgtttt tttcctcaat ttcagtaaag cttttaccgt tgtacagagt cattatcatt
     4381 gccgtttcat cgggtgttat tttcatataa cccgagtcgg ccgtggttac cgaaatattt
     4441 ccttttttat cgcggtggtc gtagataatg attccatcga ggcggtttgt tacagggtcc
     4501 ttggcggtta ccttaatgct gaaaccatcg atgtcgttgt tgaaagaccc tgcctgaaga
     4561 ttgatatcag gccgttttct ccttatgtcg taaagaagca ttctggcctg gcggtttgag
     4621 taaggcagaa catagtttga aaaaaggaat gaaattatta ccagtattcc aatcaggaac
     4681 atgaggggcc tcattattct catcaggga
//
LOCUS       DAOSPX010000262         4697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16551_length_4697_cov_2.775528, whole genome shotgun sequence.
ACCESSION   DAOSPX010000262 DAOSPX010000000
VERSION     DAOSPX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16551_length_4697_cov_2.775528"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLZ75_11240"
     CDS             complement(<1..847)
                     /locus_tag="PLZ75_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS73007.1"
                     /translation="MKRRIFLKTAALGSAGMASSVYFPRPADDRKIKIGLIGAGWYGM
                     VITTAALKSGNVEVAGICDIDSEHLSSAASQIEKLQGTRPETFRHYEDLIGLKGLEAL
                     FIGTIPHWHALQFIAGCEKGLDIYCEKPLSYDVMEGIAMVNAWKKAGNIVQIGFQRRQ
                     SGAFRKAGELIREGRIGDVHQIVAQIHYNPGIQDTRIQAPPPSLDWDAWCGPAPLLDY
                     RPSIGHRSWRLEKEYGNGHLVDWGIHHIDIIRKIMDEGMPDAFHASGGIFALKDQITT
                     PDTLTA"
     gene            1079..2389
                     /locus_tag="PLZ75_11245"
     CDS             1079..2389
                     /locus_tag="PLZ75_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase-maturation protein"
                     /protein_id="HOS73008.1"
                     /translation="MLKPAGPACNLNCNYCYYLEKSKLFGNPATTRMSDELLEEFTRQ
                     FIEAHEVPVVMFTWQGGEPTLMGLDFFRKAIEYQKKYSGGKRIDNAFQTNGTLLNDDW
                     CRFFKDNNILVGISIDGTEKNHDHYRRTRSGAPTFKRVMKGIEFLHRHSVEFNTLSVV
                     NDYNVSMASETYRFLKKTGSGFMQFLPVVERISDNTDDKSLKLVAPVHGEAKLAPWTV
                     GARDYGEFLITIFDEWVRNDVAKYYVQIFDATLANFAGENPGICVYNETCGDALAMEH
                     NGDVYSCDHFVYPDYYLGNIYETPLIDMVKSQRQFDFGIDKRNSLPRYCLTCDVRFAC
                     HGECPKHRFLTTPEGTPGLNYLCEGLKMFFNHVKPYMEFMTRELKAKRPPANVMNWIR
                     NRENQAVRQARPERNDPCPCGSGRKFKNCCAGKPPWSFQAAGNL"
     gene            complement(2363..3820)
                     /locus_tag="PLZ75_11250"
     CDS             complement(2363..3820)
                     /locus_tag="PLZ75_11250"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOS73009.1"
                     /translation="MTLSTENILLIGSVLLFISILAGKTSFRLGIPTLILFLVVGMLA
                     GSEGIGRISFDNPGVAQFIGVFALCFILFSGGFETDWVTVKPVFGKALTLSIFGVLLT
                     AGILGLFVWWITDFTLYEALLLGSIVSSTDAAAVFSILRSKNLALKGHIRPTLEFESG
                     SNDPMAYILTIVFIGLIQSGEGINASVVFMFFKQMIIGGISGFLFGIAGKRINNRIKL
                     DYEGLHPVLIISIMLMTYSITTAISGNGFLAVYLAALYLGNSELLHKRTIMMVFDGMA
                     WLMQIILFVTLGLLVFPSRIIPFIGVGMLISGFLILVARPVSVFLCLIPFRIQMRVKT
                     FYAWVGLRGAVPIVFATFPLMAGLEKADMIFNIVFFISLTSVLIQGTSIPWVARLLHL
                     ALPAKVKPVTPADVLLSEPINSAMAELVIPAHSESVGLKIVELKFPPNARIALIKRDD
                     KYIIPEGETEIHSGDKLFLIATTKDILSQVTSRLE"
     gene            complement(4187..>4697)
                     /locus_tag="PLZ75_11255"
     CDS             complement(4187..>4697)
                     /locus_tag="PLZ75_11255"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS73010.1"
                     /translation="SGPQKAWMSYSRPPEELYNVKADPFQIKNLVNDEKLKPVLDDLR
                     KRHEQWTLETGDMGHLNEPEMIERMWPGGQQPITDIPYFIINSPEERASGNYRTGGTY
                     SSPMTLGFYCPTHGASIVYSLDEKEDTRWKLYSGPLHLKPGIHNIRVRAVRYGYKDSD
                     ELKGTFVIK"
BASE COUNT         1234 a         1135 c         1084 g         1244 t
ORIGIN      
        1 cggcagtaag cgtatcgggt gtcgttatct ggtctttcaa ggcaaagatg ccacccgatg
       61 catgaaacgc atccggcatt ccttcatcca ttatcttcct aattatatca atatggtgta
      121 tcccccagtc aacgagatga ccgtttccgt attccttttc aagcctccat gaccggtgac
      181 ctatactcgg acggtagtcg agcaggggtg cgggcccgca ccacgcatcc cagtccaggg
      241 aaggaggcgg agcctgaatc cttgtatcct gaataccggg attatagtgt atctgtgcaa
      301 caatctgatg aacatcccct atccttccct ctcttatcag ttccccggct ttcctgaatg
      361 ctccgctctg gcgccgctga aaaccaatct gaactatgtt tcctgctttt ttccaggcat
      421 ttaccattgc aataccttcc atcacgtcat aggaaagggg tttttcacaa tagatatcaa
      481 gtcctttctc acaacccgca atgaactgaa gggcatgcca gtggggaatg gttcctataa
      541 acagtgcttc cagccccttg aggcctataa ggtcctcata atgcctgaac gtttccggac
      601 gggtgccctg cagcttttca atctgagagg cagctgaact gagatgctcg ctgtcaatgt
      661 cgcatatgcc ggcaacttcc acattgcctg acttaagagc cgctgttgtg ataaccatcc
      721 cgtaccagcc ggcaccaatg agaccgattt ttattttacg gtcatccgcc ggccggggga
      781 agtagactga tgaagccatc cccgcagatc ccagagctgc ggttttcaga aagatccgtc
      841 ttttcatact ttttcgggtt taagaatttt gaaataaact attcactaaa ttataatttt
      901 tccctcaata tactcaatac aggaagtctt tcttcccctt ctccgcatcc tgctttatat
      961 tatcatttat tgctctttct ccggggaata ttacttttgt atttatatgc agaaaggaaa
     1021 atcaactctc ctgttcactg atgccctgaa gctgaagcgt cccaacgctt tccaggtgat
     1081 gctgaaacct gcaggtccgg cctgtaacct taattgcaat tactgctatt atcttgaaaa
     1141 gagcaaactt ttcggcaatc cggcaaccac ccgtatgtca gacgaactcc tcgaagaatt
     1201 caccaggcag tttatcgaag ctcatgaggt tccggtggta atgttcacat ggcagggagg
     1261 cgaaccaacc ctgatgggtc tggatttttt ccggaaagcc attgagtatc agaagaagta
     1321 ctccggcggc aaacggattg acaatgcttt tcagacaaac ggcaccctgc tgaatgacga
     1381 ctggtgcagg tttttcaaag acaacaatat ccttgtcggg atatccattg acggcaccga
     1441 aaagaatcac gaccactaca ggcggacaag gtcgggtgct cctactttca aacgggtaat
     1501 gaagggcata gagtttcttc acaggcacag tgttgaattc aataccctca gcgttgtaaa
     1561 tgattataac gtgtcaatgg cttctgaaac ttaccggttt ctcaagaaga caggaagcgg
     1621 cttcatgcag tttctgcctg tggttgaacg gatatcggat aacacggatg ataaatccct
     1681 gaaacttgtt gctcccgttc acggggaggc aaagctggca ccatggactg tcggggcaag
     1741 ggattacgga gaatttctga tcacgatatt tgatgaatgg gtaaggaatg atgttgcaaa
     1801 atattatgtt cagatattcg atgccacact ggcaaacttt gcaggcgaga atccgggtat
     1861 atgtgtatat aacgagacct gcggggatgc actggcaatg gagcataatg gcgacgtgta
     1921 ttcctgtgac cattttgtgt atcctgacta ttacctggga aacatttacg agactcctct
     1981 tattgatatg gtgaaatcgc agaggcagtt tgatttcggt attgataaaa ggaacagtct
     2041 tccgcgttac tgcctcacat gcgacgtcag gtttgcctgc catggcgaat gtccgaaaca
     2101 cagattcctc actacgcccg aaggaacgcc cggtctgaac tacctgtgtg aaggactgaa
     2161 aatgtttttc aatcatgtta aaccatatat ggagtttatg acccgggaac tgaaggcaaa
     2221 gcgtccgcct gcaaacgtga tgaactggat aaggaacagg gagaatcagg cggtaaggca
     2281 ggcccggccc gagcgcaatg atccctgtcc ctgcggaagc ggcagaaaat tcaagaattg
     2341 ctgtgccggc aagcctccgt ggtcattcca ggcggctggt aacctgtgac aggatatcct
     2401 tggttgttgc tatcagaaac agcttgtcac ccgaatgtat ttccgtttca ccctcaggga
     2461 tgatgtactt gtcatctctc tttatcaggg caatccttgc attgggaggg aatttgagtt
     2521 caactatctt gagcccgacc gactctgaat gagccggaat gaccagctca gccattgctg
     2581 aattgatagg ctccgacagc agaacgtccg cgggcgttac aggttttact tttgccggca
     2641 gggccagatg gagcaatctt gcaacccatg gtattgatgt gccctgaatg aggacggatg
     2701 tcagggaaat aaagaataca atattgaata tcatatctgc cttttccagc ccggccataa
     2761 ggggaaatgt tgcaaagaca atgggtaccg ctcccctgag cccgacccat gcatagaagg
     2821 tcttcaccct catctgtatc ctgaacggaa taaggcaaag gaacacgctg accggcctgg
     2881 ccacaagaat cagaaatccg gatataagca tgcccacacc tatgaagggt attatccggc
     2941 tcgggaaaac cagcagtccg agggtgacaa acaggattat ctgcatcagc catgccattc
     3001 cgtcaaaaac catcattata gtccgtttgt gcagcagttc actgttgccc agatagaggg
     3061 cagccagata caccgccagg aacccgttgc ccgatattgc cgtagtaatg gaataggtca
     3121 tcagcatgat ggaaattatc agaacgggat gaagtccttc ataatcaagt tttatcctgt
     3181 tgttaatacg ctttcctgca ataccgaaaa ggaatccgct gattccgccg ataatcattt
     3241 gcttgaaaaa cataaaaacc accgaggcat ttattccttc tccgctctgt atcagaccta
     3301 tgaagactat cgtgaggata taggccatcg gatcattgct tccgctctcg aattcgagag
     3361 tgggccggat atggccttta agggcaaggt tttttgatct gagtattgaa aagactgctg
     3421 ctgcatccgt tgaggatact attgatccca gcagcaaggc ttcataaagg gtgaaatcgg
     3481 ttatccacca gacaaaaagg cccaggattc ccgcagtaag caatactccg aatattgaaa
     3541 gggtgagtgc cttgccgaat accggtttta ccgtcaccca gtctgtttca aatcccccgg
     3601 agaaaagtat gaagcacagg gcaaatactc cgatgaattg tgcaacgccc ggattgtcga
     3661 aggatatcct gccgatgcct tcacttccgg caagcattcc gacgacgaga aaaagtatta
     3721 gagtgggaat ccccagacgg aatgaggtct tgccggccag aatgctgata aaaagcagta
     3781 cggaaccgat aaggagtatg ttttctgtgc taagcgtcat ctgctgacaa aattacctca
     3841 tgtttcagat cttttcaagt agaggtatcc gccgttttcc gcttcacggt ttccggactt
     3901 tccgggaact gttccgcata acggaatctc atggtcaata ccttattgtt ttacatgaaa
     3961 agagacctgc aaattcaaat cgtttatgaa ttaatccatt caaaatccga tttttgaatt
     4021 gccaaatata atattttcag attaaaatat tccgtatgtt aataaaaggc aacttataac
     4081 ccgatatata atgcccggta acatgattga acatgcgtcc gcataatccg aagatctttt
     4141 cattattgca agggttacta tgagcgcaag ctgtcccgcg gaaaattcat ttgatgacaa
     4201 atgtcccttt cagctcatca ctgtccttat agccgtatct tacggccctg accctgatat
     4261 tgtggattcc cggtttaaga tgaagtggtc cggaatatag cttccatctg gtatcttcct
     4321 tttcatcaag agaatagacg atcgaggctc cgtgagtcgg gcagtagaaa ccaagcgtca
     4381 tgggcgacga gtaggttcct cctgtcctgt aattccctga ggcccgttct tcaggcgagt
     4441 tgattatgaa atagggtatg tccgtgatcg gctgttgccc tccaggccac atcctttcta
     4501 tcatctcagg ctcattaagg tgtcccatat ctcccgtttc cagcgtccac tgctcatgcc
     4561 gcttacgcag atcatccagc accggtttca gcttttcgtc atttacgagg ttttttatct
     4621 ggaagggatc tgcctttaca ttataaagtt cttcaggagg ccggctgtat gacatccagg
     4681 ccttctgcgg accacta
//
LOCUS       DAOSPX010000263         4675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16662_length_4675_cov_4.398918, whole genome shotgun sequence.
ACCESSION   DAOSPX010000263 DAOSPX010000000
VERSION     DAOSPX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16662_length_4675_cov_4.398918"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLZ75_11260"
     CDS             complement(<1..861)
                     /locus_tag="PLZ75_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS73011.1"
                     /translation="MKKINLIAILIMFAVAGSLSTTFTSCSKKAGKNIGLQLYSVRDS
                     IRNDVPGAVEKVGKMGYTFVEAAGYGDGKFYGLEPAEFRALCEKNGLYLLSSHSGRQL
                     PGEDNYAETMEWWDQCIDAHVKAGAKFIVQPSMGRSAYESIAGLREWCDYFNEIGAKC
                     NEKGIRFGYHNHDREFSTTHGDTIIYDFMLQNTDPAKVMFQIDLYWCVVGGKNPVDYF
                     NKYPGRFELWHIKDKEEIGASGMMDFKSIWAGKDISGMKYGIVEVEHYNFDQFTSCKK
                     SLDFLNEADYV"
     gene            1018..1902
                     /locus_tag="PLZ75_11265"
     CDS             1018..1902
                     /locus_tag="PLZ75_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS73012.1"
                     /translation="MMQSRRTFIKNSLSAGAGILAVSHLSCGRNEKSSFLNEIGICTG
                     VSNYQILKNAGYSYIEDSVTGFLVPLEEESVFNEKLDILRSSGLPMPALNSFIPGKLR
                     SVGPEAAHDEILEYVETAFRRAGISGVKHIVFGSSGSRKVPEGFSRDEALDQFTALCR
                     KMGPVAEKYDVVVVLEPLNKGECNLLNSVAHGGEIVKAVSHKNIMLLADVYHMLRENE
                     SPESIIRYGDYIRHAHIAENRERAAPGTYNEDFRPYLRALKEIDYKGRMSIECNWKNL
                     EEQAGPALEVLRKQIESI"
     gene            complement(1969..3375)
                     /locus_tag="PLZ75_11270"
     CDS             complement(1969..3375)
                     /locus_tag="PLZ75_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOS73013.1"
                     /translation="MKIKLSVLLLAMFSFNVINAQMPEDVFRKPLKEVLTDVERRYDL
                     KLLYSENLVKDLEVSYATWRYRMDPEETLANILLPLDLVYQKTADKTYQISRFSYYQR
                     SPEEGRKHLDRLLSSYPGLQNWEARKAELRKCILEQLGLSPMPGKKPLNPIYTAKRKF
                     DGYTVENVGIETIPGFWLCGSLYRPAKGKGPFPAVLSPHGHFSSKDLNENGRYRPEQQ
                     IRCAMLARMGAVVFSYEMFGYGESLLQIGPEDHRTSFSSSMQTYNSIRVLDFLTSLPY
                     VDAGRIGVTGESGGGTQTFLLTALDDRVTVSVPVVMVSSYFQGGCACESGLPVHSCTD
                     LMTNNVEIAAMASPRPLMLISDGQDWTAHNPEIEFPYLQKVYGLYGKEGNVENVHLPD
                     EGHDYGVSKRMPMYDFMARHLGLDLKAVRDRQGNIDESKVTIEKYEALLVFGREGKQP
                     AHALKGLDRIKEVFRSLK"
     gene            complement(3481..>4675)
                     /locus_tag="PLZ75_11275"
     CDS             complement(3481..>4675)
                     /locus_tag="PLZ75_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73014.1"
                     /translation="DSKEAGTGNKDTGAGNKDTGAGSKDTAADNKETGTGTRGAVSAG
                     EPVKIVVKGSHLRTEADKGSVVVIFADIADREGNPVKGTDRVFTWNVSGPATLAGCPV
                     YVPDDGSNIIRSDGNAGIITVTVSSPGLASGSVTIEALAGQPDNTVVDEPVLSDVGRK
                     RVFVPEQASVFSSESGLELDIIYKDIDLGRKKLSEYRREIGRIISENNPPADSASIEY
                     RTLVSLLAAHLEKNGGILSAYDYNYNIGHYNNCRVITGYVNPLKLPQLYKDWLKRYYA
                     EEMITKGYRKNAIDEMNWLNWIPSGGTVAVRQKAGHHLWPKGTVITATGDLAEMIAAV
                     HPVFRKYSREAKERALVFIAKMNPYLNKGEGGSQQDSSKAGNFKYTAGESVPVLIPFL
                     KFISE"
BASE COUNT         1170 a         1148 c         1034 g         1323 t
ORIGIN      
        1 cacataatcg gcctcattca ggaaatcaag gctctttttg cagcttgtaa actgatcgaa
       61 attatagtgt tctacctcca cgatgccgta tttcattccc gagatatcct ttccggccca
      121 tatggatttg aaatccatca ttccgcttgc gccgatttct tccttgtcct tgatatgcca
      181 cagttcaaaa cgtccgggat atttgttgaa ataatccacc gggtttttcc ctccgaccac
      241 acaccagtac aggtcgatct ggaacattac cttcgcagga tcggtattct gaagcatgaa
      301 gtcgtaaatt atcgtatccc catgggtagt tgaaaactcc ctgtcgtgat tgtggtatcc
      361 gaaacggatt cccttttcgt tacatttcgc gcctatctca ttgaaatagt cacaccattc
      421 cctgaggccg gcaatgcttt cgtaggctga ccgtcccata gagggctgaa cgatgaattt
      481 tgcaccggcc ttaacatgtg catctatgca ctgatcccac cattccatgg tttctgcata
      541 gttatcctca ccgggaagct gacggccgga gtgtgaactg agcaggtaga gtccgttctt
      601 ttcacaaaga gccctgaatt cggctggttc cagaccgtag aatttaccgt cgccgtaacc
      661 ggcagcctca acaaaggtgt agcccatttt ccccactttt tcaacagctc ccggaacgtc
      721 attccttatt gagtcgcgga cggagtaaag ctgcaggcct atattcttac cagccttctt
      781 actgcaggaa gtgaatgtgg ttgacaggga gccagccacg gcaaacatga ttaaaatggc
      841 aatcaggtta atttttttca tttctgttaa ttttaagttt gctgaaagat actaattaat
      901 tttaagctga catgccggca agcctgatca tttgacttct gccgttcagc aggcagttgt
      961 atttatagtt ttaataccat atattcggtt catcaaacaa aattaattaa taaattcatg
     1021 atgcagtcaa gaagaacttt tataaaaaac tctttgtcag caggtgcggg gattctggca
     1081 gtttcccatt tgtcatgcgg caggaatgaa aaatcttcct tccttaacga aataggtata
     1141 tgtacaggcg tttcaaacta ccagatcctg aaaaatgcgg gttattcata tattgaagac
     1201 tcggtaacag gttttcttgt cccgcttgaa gaagaatccg tcttcaatga gaaactggat
     1261 attctcaggt catccggcct gcccatgcca gcccttaatt ccttcattcc cggaaaactg
     1321 aggagtgttg gaccggaagc tgctcacgat gaaatacttg aatatgtcga aactgccttc
     1381 cgcagggccg ggataagcgg agtaaagcat attgttttcg gaagcagcgg atcgagaaaa
     1441 gttccggaag gtttctcaag ggatgaggct ctggaccagt ttaccgcact ctgcaggaaa
     1501 atgggtcctg ttgcagaaaa atatgatgtg gtggttgttc ttgaacctct taacaaagga
     1561 gaatgcaatc tcttgaattc cgttgcccac ggtggtgaaa tagtaaaggc tgtcagccac
     1621 aagaacataa tgctgctggc cgatgtatat catatgctta gagagaatga aagcccggaa
     1681 agcataatca gatacggcga ttacatacgc catgcccata ttgcggagaa cagggaaaga
     1741 gccgctcccg gaacatataa tgaagatttc cggccctacc tcagagccct gaaggaaata
     1801 gactataaag gccgtatgtc aatcgaatgc aactggaaaa accttgagga acaggctggt
     1861 cccgcactgg aagttttacg gaaacagatt gaatccatat aacagtcagc tttcccgtaa
     1921 gcacttgtag tttgtatata cttagagcag ccggatttgc agcagctttt acttaagaga
     1981 cctgaatacc tcctttatcc tgtccagtcc cttgagtgca tgggcaggct gtttcccttc
     2041 cctgccgaaa accaggagag cttcatattt ttcgatagtc acctttgatt catctatatt
     2101 gccctgcctg tccctgaccg ccttaaggtc cagtccgaga tggcgggcca tgaaatcata
     2161 catcggcatt cttttcgaaa caccataatc atgaccttca tcgggcagat gaacgttttc
     2221 aacatttccc tccttgccgt aaagcccgta cactttttgc agatagggaa actctatttc
     2281 cggattatga gccgtccagt cctgtccgtc cgaaataagc ataaggggac gtggtgaagc
     2341 cattgccgct atttcaacgt tgttggtcat cagatcggtg catgaatgaa ccggcaatcc
     2401 gctttcacat gcacatcctc cctggaaata gcttgacacc attaccacag gaacgcttac
     2461 agtcaccctg tcgtcaaggg ccgtaagaag gaatgtctgt gtgcctcccc cggattcccc
     2521 ggtaacacct atccttccgg catcaacata tggaagtgac gtgaggaaat ccagcactct
     2581 gatggaatta tatgtctgca tgcttgatga aaaagatgtt ctgtggtctt cgggaccaat
     2641 ctgtaacagg gattccccgt agccaaacat ctcatagctg aataccactg ctcccattct
     2701 ggcaagcatt gcacatctta tctgctgttc gggccggtag cggccgttct cgttaaggtc
     2761 cttgctgctg aaatgtccgt gtggtgacaa tactgccgga aacggtccct ttccttttgc
     2821 aggacggtag agggatccgc agagccagaa cccgggaatg gtttcaatac ctacattctc
     2881 cacagtatag ccgtcaaatt tccgtttcgc tgtatatatg ggattgaggg gcttctttcc
     2941 gggcatggga gaaagaccga gctgttcaag aatacatttt ctgagttcag ccttgcgggc
     3001 ctcccagttc tgcagtccgg gatagcttga cagaagcctg tcaagatgtt tcctgccttc
     3061 ttccgggctt ctctgatagt aggaaaacct gctgatctga taagttttgt cagctgtttt
     3121 ctgataaacc agatcaagcg gaaggagaat attggcaaga gtctcctccg gatccattct
     3181 gtaacgccag gtggcatagc tgacttccag gtcctttaca agattttcac tgtacagaag
     3241 ttttaaatca tatcttcttt caacatcagt cagaacttcc ttaagtggtt tgcggaaaac
     3301 gtcctccggc atctgggcat taatcacatt aaatgaaaac atggcaagaa gcagaacaga
     3361 taattttatt ttcatgattt acagtttttt atttgtgttt catcctcttc cggtataaaa
     3421 gtccgtacag ccttaagtcc gcttgttacc ggcatgatcc cggccgcagg ttttttatct
     3481 ttattcggag ataaatttaa gaaaaggaat caatactggc actgattctc cggcagtata
     3541 tttgaaattc cctgccttac tgctatcctg ttgactgccg ccctctccct tatttaaata
     3601 tggattcatt ttggcaatga aaacaagggc gcgttctttt gcctcgcggc tgtattttct
     3661 gaaaacgggg tgaacagccg ctatcatttc agcaagatcg cctgttgcag tgattacagt
     3721 tcctttgggc cacaggtgat gcccggcttt ctgacggacg gcaacagttc cccctgacgg
     3781 aatccagttg agccagttca tttcgtcaat ggcgtttttc ctgtacccct ttgtaatcat
     3841 ttcctccgca taatatcttt tcagccagtc cttgtacagc tgcggtagct tcaagggatt
     3901 tacataaccg gtgatgaccc tgcaattatt ataatgacca atattgtaat tgtaatcgta
     3961 tgcgctgagt atccccccgt ttttctcaag atgggctgca aggagggaaa caagggtacg
     4021 gtattctatg gaggctgaat cggccggggg attgttttct gaaatgatcc ggcctatctc
     4081 cctgcggtat tcagacagct ttttcctgcc caggtcaata tccttatata taatatccag
     4141 ttccagtcct gattcagaag aaaagacgga agcctgttcc ggaacaaata ccctttttct
     4201 gcccacatca ctgagaaccg gttcatcaac cacggtgtta tccggttgtc cggcaagcgc
     4261 ctcaatggtc accgaaccgg atgcaagtcc cggtgaagaa acagtgactg tgatgattcc
     4321 ggcattgccg tctgatctta ttatatttga accgtcgtcc ggaacataaa caggacagcc
     4381 tgccagtgtt gccggtccgc tcacattcca ggtgaacacc ctgtctgttc cctttacggg
     4441 attcccttcc ctgtcggcaa tatccgcgaa aataacaaca acagagcctt tgtccgcctc
     4501 cgttctgagg tgactgcctt taacaacgat tttaaccggc tctccggcac taacggcccc
     4561 tctggtacca gtaccggttt ctttgttatc agctgcggta tctttgctgc cagccccggt
     4621 gtctttgtta ccagccccgg tgtctttatt gccagttcct gcctctttgc tgtca
//
LOCUS       DAOSPX010000264         4666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16704_length_4666_cov_4.506398, whole genome shotgun sequence.
ACCESSION   DAOSPX010000264 DAOSPX010000000
VERSION     DAOSPX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16704_length_4666_cov_4.506398"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2341)
                     /locus_tag="PLZ75_11280"
     CDS             complement(<1..2341)
                     /locus_tag="PLZ75_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOS73015.1"
                     /translation="MHYLKNTIPVLISCLISVSALNAGSEDPPFLKYMDHPWVDSVMK
                     TMTPEQRTGQLIWVAAFAQGDLEHEAWLHDMVRNQGIGGLIFFRGEPVRQAEMINYYQ
                     KISKVPLMIVTDGEWGLGMRLKGIEKFPYQLTLGALENDSLIYEMGKAVAGQFKRAGV
                     NINLAPVADINNNPGNPVINFRSFGENPERAAHKAGMYMKGLQDNGIISVAKHFPGHG
                     DTEVDSHTDLPVINHSRERLNSVEFLPFRSLINAGISGIMSAHISLPLIDDLDNRPAT
                     VSERIITGLLKNELSFRGLVLSDAMNMKGITDFTTPGEAEVSALVSGIDVLEYVTEPE
                     KAIAAIMDKINRKQISQGMIDEKCRKVLAAKYWAGLNSLREIDTGNVSEEIMPPADRA
                     LVRDLYASSLTLLRNENNILPVKNLDKARIAVLSIGKNTPDRFCERSGDYTSVSGFRA
                     DPADMRNADRILDTLSHYDIVLAGIYNTDQSPRAGFGIDSHLPAFLDRLVSSHKTVAV
                     YFGNPYALNKLEALQKADGLILAYEENDYTEDLSAQLIFGGTGASGTLPVTINDRWTA
                     GYGLKTSGSQRLQYGFPENAGLSSAILSARIDSLAETGLAAKAYPGCEVIVARKGIVV
                     FHKTYGYQTYDKRVEMKEGDLYDLASVTKISSSLPGLMILETEGRFSTEGKLGDYIPE
                     FRHSDKGELVMKDLLAHQAGLTPSIVPWRKTIKRDSVYKRNTIRYVPSERFPVKISDK
                     LYINRNFRDKMFNEIKHTRLGEKKYVYSDLTFILTPEIVE"
     gene            2656..>4666
                     /locus_tag="PLZ75_11285"
     CDS             2656..>4666
                     /locus_tag="PLZ75_11285"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOS73016.1"
                     /translation="MKTCFFKSLTFLTILFLLNESPAFAQSPERLQAIASEKLKKWEN
                     PLVSWQHIAVPQQDSLKIAAEEKRISLFFDPRLQYYPFREESCNLFRYSLKKSLGRKF
                     RNYEIRIFTNNYELEQLVPNYFRKSLPVDTERLPVARDGDKRVLVRNTDRIYPSMGLS
                     GNSIALWHSHGYYYEMNLDRWEFQRAKLFGTVEDITVMGYVVPYLTPMLENAGANVFL
                     PRERDIQINEVIVDNDWSHGRSELILMPDPETERVNTGFLLTDTLFSGFNPFRHGTSL
                     RIKKDSAVYVPDIPENGSYAVYVSYPLMEDNSGSVMYAVNHTGGSTRFMVDQTMGGET
                     WIYLGTFLFRKGKNIAEGSVSVKNAGNDGGPVALDAVKFGGGMGNVARKPSAEIIKNL
                     QSAVEQAPGSVTGNISGDNSRFGWKISGKPRFLEGARYYLQYAGMPDTLVYSPNACRN
                     DYNDDYQSRSLWVNYLMGEPFPDDRTQAKGLGIPLDLSLAFHTDAGITPGDSIIGTLA
                     IYSTASDEGKFPDGSSRLASRDLSDIVQTQIVEDIRKMYNPEWTRRGLWDRPYYEARK
                     PDIPAMLLELLSHQNLADLKYGLDPKFRFDVSRAVYKGILKYLAWTEGRDYVVQPLPV
                     KDFAIMPAGEKTVRLSWKPVTDENEPSSVPERYRVYMRIGGKGFDN"
BASE COUNT         1205 a         1076 c         1120 g         1265 t
ORIGIN      
        1 tctcaactat ttccggtgta aggatgaatg tcagatccga gtaaacatat ttcttttcac
       61 ccagacgggt atgttttatc tcattgaaca ttttatccct gaaattccgg tttatataca
      121 gtttatccga aattttcacc gggaatcttt ccgatggcac atacctgatt gtatttctct
      181 tatagactga atccctttta attgtttttc tccacggaac aattgaagga gtcagtccgg
      241 cctggtgggc cagaagatcc ttcatcacca gttctccctt gtccgagtgc ctgaattccg
      301 gtatgtaatc accgagtttc ccttcggttg agaagcggcc ttctgtttcc aggatcatga
      361 gtccgggaag ggaggaggat atttttgtga cagaagccag gtcatacaga tccccctctt
      421 tcatttcaac cctcttgtcg taggtctgat acccgtaggt tttgtggaaa acaacaattc
      481 ccttccttgc gactatcact tcacagcccg gataggcctt tgctgccagg cccgtttctg
      541 ccagtgaatc gatacgggca ctgagtatgg ctgaagaaag acctgcattt tcagggaacc
      601 cgtactgcag ccgctggctt ccgctggtct tcagtccgta tcctgccgtc cacctgtcat
      661 ttattgtaac cggcagagtt ccggaggctc ccgttccgcc gaaaatcaac tgggctgaga
      721 ggtcttcagt ataatcattt tcttcatatg caagaatgag tccgtcagcc ttctgcaatg
      781 cttcaagttt attcagggca tagggattcc cgaaatacac tgcaacggtt ttgtgagagc
      841 taacgagcct gtcaaggaag gcgggcagat ggctgtctat accgaaaccg gccctgggac
      901 tctggtcggt gttgtatatt ccagcaagca ctatgtcgta atgagaaagg gtatccagga
      961 tcctgtccgc attccgcata tctgcaggat cagcccggaa accggataca ctggtgtaat
     1021 ctcctgatct ttcacagaat ctgtccgggg tgttttttcc gatggaaagg acagctatcc
     1081 tggccttgtc aagatttttc accgggagga tgttattctc gttcctgagc agcgtaagcg
     1141 aagatgcata aagatccctg acaagagccc tgtctgcagg cggcatgatt tcttcggaaa
     1201 catttccggt atcaatttcc cgcagactgt ttaatccggc ccagtatttg gctgccagaa
     1261 ccttacgaca cttttcatca atcattccct gcgaaatctg cttcctgttg attttgtcca
     1321 taatggcggc aatggctttt tcgggttcag tgacatattc cagtacatct attcccgaaa
     1381 ccagggcact gacttccgcc tctccgggcg tagtaaaatc agttatgcct ttcatgttca
     1441 ttgcgtcgct aaggaccaga cccctgaagg aaagttcgtt tttgagcagg ccggtgatga
     1501 tccgttccga taccgtggcc ggcctgttat caagatcatc tatcagcggc agactaatat
     1561 gtgcggacat tattccggat atcccggcat tgataaggga cctgaaggga aggaattcaa
     1621 cggagttaag cctttcccgt gaatgattta taacgggcag atcagtatgg gaatcgactt
     1681 cagtatctcc gtgtccggga aaatgcttgg ccactgatat tataccatta tcctgcaggc
     1741 ccttcatata catgcctgcc ttatgtgctg ccctttccgg attttccccg aaggaacgga
     1801 aattaatgac gggattcccg ggattgttgt taatatcggc aacaggtgca agattgatat
     1861 ttacaccggc tcttttaaat tgtcctgcaa ctgctttccc catctcatag atgagggagt
     1921 cattttccag tgcgcccagg gtaagctgat agggaaattt ctcaatcccc tttagtctca
     1981 tgccgagtcc ccattcgccg tcagtcacga tcataagggg aacttttgat atcttctggt
     2041 aataattgat catttcagcc tgcctgacgg gctccccgcg gaaaaagata agtcctccga
     2101 taccctgatt cctgaccatg tcatggagcc aggcctcatg ttccagatcg ccctgtgcga
     2161 aggcagcaac ccagatcagc tgccctgtcc tctgttcagg cgtcatggtt ttcattaccg
     2221 aatcaaccca gggatgatcc atgtatttta aaaacggagg atcttcgctg ccggcgttga
     2281 gggcagaaac tgatatcagg caggaaatca gaaccggtat tgtgttttta agataatgca
     2341 ttgttcttaa atatgtttat tgatgtttat aattaatccg gatttcaggt cctgaatccc
     2401 ggggaagttt catttatgaa aaacaatgat cgctctcctg tctgaaggga gatttctccg
     2461 taaaacctgc cggcagggca ttattttttt aatatcacgg atctttaatc cgctgccggt
     2521 atcattttcc ggaaaaattt aaactgtgtg caaaaataag gtaaataaat tgtttgtcct
     2581 taaattgatg ccagatttaa aaagacagga agtctggtca ataattatta tatttcaatc
     2641 ccaaaaaaaa tgcggatgaa gacctgtttt ttcaaatcac tgacattttt aactattctt
     2701 ttccttctga atgagtcacc ggcatttgcc caatctcctg aaagattgca ggcgatcgca
     2761 tctgaaaaac tgaaaaagtg ggaaaatcca cttgtttcat ggcagcatat tgctgtgccg
     2821 caacaggatt ccctgaaaat tgccgcagaa gagaaaagga tatccctttt cttcgatccc
     2881 cggctgcagt attatccctt cagggaagag agctgcaatc tgttcagata ttccctgaag
     2941 aaatcactcg gaaggaaatt cagaaattat gaaatcagga tttttaccaa taattacgaa
     3001 ctggagcagc tggttcccaa ctatttcaga aaatcactgc ctgttgatac ggaacggctt
     3061 cccgttgccc gggacgggga caaacgtgtg ctggtaagga atactgaccg gatttatcct
     3121 tccatgggac tctcaggtaa ttcaattgca ctgtggcaca gtcacggcta ttactatgaa
     3181 atgaatcttg acagatggga atttcaaagg gcaaagctgt ttggcacggt tgaggatata
     3241 acggttatgg gctatgtggt accctatctt acccctatgc tggaaaatgc aggggcaaac
     3301 gtattccttc caagggaaag ggatattcag attaacgagg tgatagtgga taatgactgg
     3361 tcgcacggcc gttcggaact gattctgatg ccggatccgg aaactgaaag agtgaatacg
     3421 ggctttcttc ttacagacac ccttttttcc ggattcaacc ctttcagaca tggcacttcg
     3481 ctgcgcatta aaaaagattc ggcagtatat gttcccgata ttcccgaaaa cggatcatat
     3541 gcagtgtatg tctcataccc tctcatggag gataactccg gatcggttat gtatgcggta
     3601 aaccatactg ggggcagtac ccgtttcatg gttgaccaga ccatgggagg tgaaacatgg
     3661 atatatctcg ggacatttct tttcaggaaa ggaaaaaata tcgcggaagg ttcggtaagc
     3721 gttaaaaacg cgggtaacga cggtggaccg gttgcacttg atgccgtgaa attcggcgga
     3781 ggcatgggaa atgttgccag gaaaccatca gctgagatca taaaaaatct gcagtcggcc
     3841 gtggaacagg ctccgggatc agttacgggc aatatctccg gggacaactc ccgctttgga
     3901 tggaaaataa gcggaaaacc gcgtttcctg gaaggggcaa ggtattacct gcaatatgcc
     3961 ggaatgccgg ataccctggt ttacagtccc aatgcctgca ggaacgacta taacgacgac
     4021 taccagtcga ggagcctctg ggttaactac cttatgggtg aaccctttcc tgatgacagg
     4081 acacaggcga aagggctggg aattcctctt gacctgtcac tcgcgtttca tacggatgca
     4141 ggtataacac cgggagattc gataatagga accctggcta tatattccac ggcatcggat
     4201 gagggaaaat ttcctgacgg aagctccagg ctggccagca gggatctgag tgatattgtt
     4261 cagactcaaa tagttgaaga tatcagaaag atgtacaacc cggaatggac acgaaggggt
     4321 ctttgggaca gaccatatta cgaagcccgg aaaccggata ttcccgccat gcttcttgaa
     4381 ctcctgtcgc atcagaacct ggcagacctg aaatacggcc ttgatcctaa attccgtttt
     4441 gatgtcagca gggctgtata caaggggatc ctgaaatatc ttgcatggac ggaaggacgt
     4501 gattacgtgg ttcagccgct tcccgtgaag gattttgcaa ttatgccggc gggcgaaaag
     4561 acagtcaggc tgtcctggaa gcctgtaact gatgaaaacg aaccttcatc cgtgccggag
     4621 cgctacaggg tctatatgcg gataggcggg aagggctttg ataatg
//
LOCUS       DAOSPX010000265         4665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16713_length_4665_cov_3.723210, whole genome shotgun sequence.
ACCESSION   DAOSPX010000265 DAOSPX010000000
VERSION     DAOSPX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16713_length_4665_cov_3.723210"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLZ75_11290"
     CDS             complement(<1..327)
                     /locus_tag="PLZ75_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73017.1"
                     /translation="MKKNKFPGKTFFSIPKKLFPILRTVVFLIISGTLQVNATDTYLQ
                     NTGIAPDLSDAVLIIDPGSDAPEPGILQQQTISGTVTDIHTREPMPGVNIVEKGTTTG
                     TITDLGG"
     gene            complement(453..608)
                     /locus_tag="PLZ75_11295"
     CDS             complement(453..608)
                     /locus_tag="PLZ75_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73018.1"
                     /translation="MKNIRQGVLDVLGVMGVQGAQGVQGLQDCGYVKLCELSDLGGFY
                     FVFDLIS"
     gene            complement(1177..2739)
                     /locus_tag="PLZ75_11300"
     CDS             complement(1177..2739)
                     /locus_tag="PLZ75_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOS73019.1"
                     /translation="MRIKIISIIAALGITILPSCNSDALTDLNRNPNVIDFIVPEYLF
                     TNILLNCSWNHGSLMDGMHYSARYKDVPDIGGKQYDYITGPGFGTYSTQLNRIQQCMG
                     ELTSPDDVNKIAALKIMRVYAYHVLTDAMGDIPYSEAQQALIGNYKPKYDTQEDIYHG
                     MFTELEDAIAMMDESKPTFKGADVFYGGDMNRWKKFANTLMLRLAMRLSNKDEVTAKA
                     RALQAINGGLLEDFSDIAYLKFSSTATNPRAPYTEYQATQDPDNAQGGKVASTFVDQL
                     KDTRDPRLYVLLVVWTKEGSVYVPDTSRAIQRGMVPGAVMGYPDDFETYSEFSPLWWN
                     RDSSPLLVLGPAEAYLLLAEAVLRGWYTGITEKEAYDLAVTRAMEQWRLWPRITGLAP
                     NINTISQNETEAYLTSGYPYKTAGTFDERLEQISIQKWVSLIGDSYEIWSNWRRTGYP
                     DFSWKNWEGNQPYPGSVTGGEFFRRMPLPDESASNHENQQEALARQGFPLDVKIKAQA
                     DALLGRVWWDKP"
     gene            complement(2760..>4665)
                     /locus_tag="PLZ75_11305"
     CDS             complement(2760..>4665)
                     /locus_tag="PLZ75_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS73020.1"
                     /translation="PTVGYAEMNANWPVYLLANTMDIRRLAPGYDPETFVETPWNPVP
                     DAPNSYFVVNRMGNEDITKRYIVSASIQVDILKNLFIIGKGQRDFQNFEYSSYVPIGT
                     LFTPFGALNTQNGQDEVTNLQSTLHYNTSFSQDFSLSAFVGVNSERTFNKINTVNGRD
                     FVIPDFISLTNLGTITTTGADFRSEVSKGTNSVFGSVDLNYRRFAYMSLTGRKDWFSV
                     LNPGTNSIFYPSVGGGLILSDVINFPKQIDFLKLRANWAQVGSATVGAGTVRQTYTIS
                     TTNGYNIPTQNVSTNLQNPDLRPLMVTTIEGGFDLKVLKNRLGLDFTYYSKVTTDDIV
                     GINISTASGFSGGNVNIGKTTNKGVEIMLTGTPVESRSPGGFSWNTSFNYAYNKSEIV
                     ELAETVAELSLGTGIQGVRIVNRPGLPYATVMAIYPAHNSDGVPVYNSTSNYPIGEER
                     VAGVGIPPHTMGWTNTLNYKNFALTFLVDSKLGAVAYQNLMFYATRFGLSKMTLPGRD
                     TGLQLTGVDENGAEYSYLWTPETIQSYYNQLGRGYSGLFTYNVDFVKLRRLVLNYNVP
                     LNKLGINGIKSMSVAFVSSNLLTLYKDKALRENGLDPEFQESTSNAQGTGGVNEPRTR
                     NLGFNIMVKF"
BASE COUNT         1154 a         1076 c         1012 g         1423 t
ORIGIN      
        1 gcctcccaga tcggttatgg ttcccgtggt ggttcccttc tccacaatat ttactcccgg
       61 catgggttcc cttgtatgaa tgtcagttac cgtcccgctt attgtctgtt gctggagaat
      121 gccgggctcc ggggcatcag atccgggatc tatgatcagt actgcatcgg aaagatccgg
      181 tgcaattccg gtgttttgca aataagtatc agttgcgtta acctgaagtg ttcctgaaat
      241 gataagaaaa acaacagtac gcagaatcgg gaagagtttc ttcggaatcg aaaagaacgt
      301 cttccccggg aatttgtttt ttttcataaa acttttgtgt ttaggttaaa aatcagtaat
      361 atttagtgct tattgagttt caacagattg aacttaactg agaaaatcat gtgattcctt
      421 ttacaaatcc ttttacagca ttttcaaata agctaagaaa taagatcaaa tacaaaataa
      481 aatcctccca aatctgataa ttcacataat ttaacatatc cgcagtcctg caggccctgc
      541 acaccctgcg cgccctgaac gcccatcacg cccagtacgt ccagcactcc ctgccgaata
      601 tttttcatac cgaattttgt ttacttctgc ctgtcactat tgataatttt cattgctgac
      661 ttttcagctc ctgagcgtct tacatgatag tcttgcctgg gttctgtttg caccgcttgt
      721 tctttcagaa ccttaatgcc atgtatgcta tgcctgattt tttctcccgt gattttttca
      781 attgaaatat atgaaagaaa aatgcaataa aaaatgatta ttgccctgaa ccggcagtac
      841 aaccggattc ttttagcaat gtatatgcac ggacggaggt tgatgaacca attcaaaaaa
      901 aacaaaggta aggaatggct cctggctcct tcagcaactg atctgatgct ttccggcact
      961 tacaacttat ttatcggcta ttatttatag gatgtaaacc ggttggaaaa acagggcggc
     1021 gatgcctcag taccttaaat tgccagcctt taagttttct cttgttcaga ataaacaaaa
     1081 agctgccccg tttattctga gacagctttt tattattgag aatcagatga gtttttctga
     1141 tccttctttc tatataaata atcagataat ttacatttat ggtttatccc accatactct
     1201 tccaaggagc gcatcagcct gtgccttgat tttcacgtca agggggaatc cctgcctggc
     1261 cagtgcctcc tgctggtttt catgattgct ggcagattca tcgggcaggg gcattcttct
     1321 gaagaattcc cctcctgtta ctgatccggg atatggctgg ttgccttccc agtttttcca
     1381 gctgaaatcc gggtatcctg tacgcctcca gtttgaccat atctcgtagc tgtcgccaat
     1441 caggctgacc catttctgaa tgcttatctg ttcaaggcgt tcatcgaagg tgcctgcggt
     1501 tttgtacggg tatccggaag ttaaataggc ttctgtttca ttctgtgaaa tagtgttaat
     1561 attaggagca aggccggtta ttctcggcca tagcctccac tgctccatgg ccctggttac
     1621 tgcaaggtca taagcttctt tttctgtgat acctgtatac catcctctca gtacggcttc
     1681 tgccagaagg agatatgctt cggcaggacc cagcaccaga agcggtgagg aatctctgtt
     1741 ccaccacagg ggagaaaact cagaatacgt ttcaaaatca tcgggataac ccataacggc
     1801 tcccgggacc attcccctct ggattgccct cgaagtgtca ggaacataga ctgatccttc
     1861 cttggtccag actaccagaa gtacatataa tctcggatcc cttgtgtcct tcagctgatc
     1921 aacgaaggtg gacgccactt tccctccctg tgcattgtcc ggatcctgtg ttgcctggta
     1981 ctcggtatac ggggccctgg gattagtagc tgttgaactg aatttcaggt atgctatatc
     2041 ggaaaaatct tccagaagtc caccgttgat ggcctgaagg gccctggctt ttgcagtgac
     2101 ttcatccttg tttgacaatc gcattgccag acgaagcatc agagtgtttg caaacttttt
     2161 ccaccggttc atatcgcctc cgtaaaaaac atcggctccc ttgaatgtcg gttttgattc
     2221 atccatcata gctatggcat cctccagctc ggtgaacatc ccgtggtaga tatcctcctg
     2281 ggtatcatac tttggcttgt aatttcctat aagggcctgc tgggcctcgg aataggggat
     2341 atcgcccatg gcatccgtaa ggacgtgata ggcatatacc ctcatgatct tgagtgcggc
     2401 aatcttgtta acatcatccg gtgaagtaag ttcacccatg cattgttgta tcctgttcag
     2461 ctgggtgctg taggtaccaa atccgggtcc ggtgatatag tcatactgtt tcccgccgat
     2521 atccggcaca tccttgtacc gggccgaata atgcatgcca tccatcaggg aaccgtgatt
     2581 ccatgaacag tttagcagaa tgttggtaaa aagatattcc gggacaataa aatcgatgac
     2641 attgggattc ctgttcaaat ccgttaacgc atctgaattg catgatggca gaattgttat
     2701 gccaagcgct gcaattatag atataatttt aattctcata tcgctaacat taaaattgat
     2761 taaaatttca ccattatatt aaagcccagg ttacgggttc tcggttcatt gactccaccg
     2821 gtaccctggg cattgcttgt tgattcctgg aattcaggat caagtccgtt ttcccttaaa
     2881 gccttatcct tatacagcgt aagcaggttg ctggagacaa aggctactga cattgatttt
     2941 attccgttta ttcccagttt gttcagaggt acattataat taagaacaag tctgcggagt
     3001 tttacaaaat caacattata ggtgaataat cctgaatagc cccttcccag ctggttgtaa
     3061 tagctctgga ttgtttcagg agtccacagg taagagtatt ctgcaccatt ttcatccact
     3121 cccgtcagtt gcagtcctgt gtctcttccg ggcagtgtca tcttggacaa cccgaatcgg
     3181 gtggcataga acatcagatt ctgataggct acagcgccca gtttggaatc aacaaggaaa
     3241 gtcagtgcaa aattcttata gttcagggtg tttgtccatc ccatcgtgtg tggtggtatc
     3301 ccgactccgg ccacacgttc ttccccgatg ggatagttac tggttgaatt gtaaacaggt
     3361 actccgtctg aattatgtgc gggatagatt gccatcacag tggcataggg caatcctggg
     3421 cggtttacaa tccttacacc ctgaatccct gttccgagtg acagttccgc cactgtttct
     3481 gccagttcca caatctcact cttgttatag gcgtagttga atgaggtgtt ccagctgaag
     3541 ccgccggggg atctggattc cacaggtgtt cccgtaagca ttatttcaac tcccttattg
     3601 gtggttttgc cgatgtttac attaccgccg gagaatccgc tggctgttga tatgttgatg
     3661 ccaacgatgt catctgttgt tactttgctg taataggtga aatcaaggcc cagacggttc
     3721 ttaaggacct tcaggtcaaa tccgccttcg attgttgtga ccataagtgg cctcagatcc
     3781 ggattttgaa ggttggttga cacattttga gtagggatat tgtatccgtt tgttgtgctg
     3841 attgtatagg tctgccttac agtgcctgct ccaaccgttg cacttcccac ctgtgcccag
     3901 ttagcgcgca gtttcaggaa atcaatctgt ttcgggaaat ttatgacgtc agacagtatt
     3961 aagcctcctc cgaccgaagg gtaaaaaatt gaattggttc cgggattcaa tacagagaac
     4021 cagtcttttc ttccggttag cgacatatag gcgaaccttc tgtagttcag gtccacagat
     4081 ccgaaaactg aattggttcc cttgcttact tcgcttctga aatcagctcc ggttgtagtg
     4141 atggtgccaa ggtttgtaag gctgataaaa tccggtatta caaaatccct tccgtttact
     4201 gtgtttatct tgttgaatgt gcgttcagaa ttcactccca caaacgcact tagtgaaaag
     4261 tcctgtgaga atgatgtatt atagtgaagt gttgactgaa ggtttgtcac ttcatcctga
     4321 ccgttttgtg tgttaagtgc tccgaagggt gtgaacaatg tgcctattgg tacatagctt
     4381 gagtattcaa aattctgaaa atccctttgt ccttttccta taataaacag attcttcagg
     4441 atatcaacct gtatgctcgc cgaaacaatg taacgcttgg taatgtcttc attccccatc
     4501 ctgtttacaa caaaatagct gtttggtgca tcgggtacag ggttccatgg tgtttccaca
     4561 aaagtttccg gatcatatcc cggagccagc cttctgatat ccattgtatt ggcaagcaga
     4621 taaaccggcc agttggcatt catttcagca tatccgactg taggc
//
LOCUS       DAOSPX010000266         4641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16813_length_4641_cov_4.156127, whole genome shotgun sequence.
ACCESSION   DAOSPX010000266 DAOSPX010000000
VERSION     DAOSPX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16813_length_4641_cov_4.156127"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLZ75_11310"
     CDS             complement(<1..189)
                     /locus_tag="PLZ75_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOS73021.1"
                     /translation="METVVSGIRSTGNLHLGNYFGAIRNFLKMQKENNCFFFIADYHS
                     LTTHPKPADLHANIRQVLS"
     gene            311..649
                     /gene="rbfA"
                     /locus_tag="PLZ75_11315"
     CDS             311..649
                     /gene="rbfA"
                     /locus_tag="PLZ75_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOS73022.1"
                     /translation="MESTRQKKVSRLIQKEIAGIFLRKGNELAPGKMISITRVRISPD
                     LSSARIYISIFPSNDNKQVMKAVNDHIPRIRYELGHQIKSQLRIVPEIAFFLDDSLDY
                     IENIDNLLKP"
     gene            712..1464
                     /locus_tag="PLZ75_11320"
     CDS             712..1464
                     /locus_tag="PLZ75_11320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS73023.1"
                     /translation="MRKTLYFLLDILLLRTWHIRKALREARGILPDDARILDAGSGFG
                     QYVWRLSRMGRNWKITGVDIDREMIEDCRDFFGRAGLSERVSFREADLTTFSDPVKYD
                     LILSVDVMEHIEDDGSVFRNFRQSLSEGGILIISTPSDTGGSGVHSPDEESFIGEHVR
                     NGYGTDEIRGKLTAAGFSSINIKYSYGVPGNISWHISMKYPVRLLNKSMAFALVLPFY
                     YLIAFPAALILNFLDLRLSHEKGSGLVIKAVK"
     gene            1465..2703
                     /locus_tag="PLZ75_11325"
     CDS             1465..2703
                     /locus_tag="PLZ75_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOS73024.1"
                     /translation="MKLALYIAKRYLFAKKSRNAINVISGVSVAGVTVGTMALIIILS
                     VFNGLELLVTAIFNTFDPDLKITAAEGKTFIPDTARLHRLSRIDGVSCYSLTLEENAY
                     LKYGDREYIGTIKGVDENYVRVTGIDSTMWEGEFLLTSENMRPWAVAGLGVANHLGMR
                     VNFVTPLFIYVPRKSAGSSMDYENAFIRKYIFPSGIFQIEQTYDSKYVYLPLDFTREL
                     IQDEEGVSSIEIKLSANADANRVRKNVNSIFGEDLVIRNRYEQQEIFYKVMRSERLAI
                     FMILTLIIIIASFNIVGSLTMLIIEKERDIEILRSLGADNKLIRKIFIFEGWLISIIG
                     TAAGVILGFIICWIQQEYGVVRLNTSTLIMESYPVVMKARDFIIIPATVLLIGFWAAW
                     YPVRFLTRKYLGKHEKGSDT"
     gene            2712..3467
                     /locus_tag="PLZ75_11330"
     CDS             2712..3467
                     /locus_tag="PLZ75_11330"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOS73025.1"
                     /translation="MNKILPAIALCLIFPSCKNEEKKGLIPLKTFSRIMYEVHLADGL
                     LSSSVIRNQYYARDSIANYRDIAESHGYTKEALDRTMKYYFTKEPKKFIKIYDNTIAT
                     LTEMELRLEEEMAVMPPLAGGMWKGQPEYHFSGLPDTNRISFDHIFHSPGIYSIKFTL
                     TLHPADQSVNPCMTGSLCPADSLADRECEFFRGPVYLKDGQAHTYIFPIRISGRLPVL
                     FRGRLLDFENRPGGHPRYGTVENISFTLSSSVL"
     gene            3464..>4641
                     /locus_tag="PLZ75_11335"
     CDS             3464..>4641
                     /locus_tag="PLZ75_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS73026.1"
                     /translation="MRRLSASLVFTNDGPPLRNGIVTTSDDGTILKVEKGGGSVPEAD
                     PDRGSSSPEPDTEFYNGIIIPGFVNCHCHLELSHLKGTIPAGKGLGPFLADLTASRKG
                     DPETIRKAAAAADRDMYTEGTELCADICNNSSTFGIKRESRISYFNLIEVFASDPALA
                     PKRMQEAENLAAESTAAGLPFSIVPHSVYSVSLPLFRLIREKSGGNLVNSIHFMESAG
                     EEMYLSDRKGPLMDSFSEAGLIPPVLQTPENCVSAILDEVTTSRSLILVHNTVAVRET
                     VRKINPRGKVYWCLCPNSNLHIEGKMPPVSMLSEEGCDIVIGTDSLASNENLSILAEL
                     KAIQQHYPSARLEDMIRWSTINGAKALGKESRYGSIAPGRKPGLLLIENADTVNMKLT
                     P"
BASE COUNT         1287 a         1105 c         1127 g         1122 t
ORIGIN      
        1 cgaaaggacc tgtctgatgt ttgcatgcag atcagccggt tttggatgag ttgtgagtga
       61 atggtaatct gcaatgaaga agaagcaatt attctccttc tgcattttga gaaaattcct
      121 tatggctccg aaatagttcc ccagatgcag gtttccggtt gacctgatac cacttaccac
      181 tgtctccata tcattgtaaa aaatccctgc aaagctaagg tttttttgat ttataacaac
      241 gctgtcccgc cccagagagg ggctgggaaa attattacta cctttgcatt gtttaaaaat
      301 ttctcaggca gtggaatcaa cacgacagaa aaaggtatcc cgcttgattc aaaaggaaat
      361 agccggtata tttctccgga agggcaatga acttgctccg ggaaagatga tctccatcac
      421 cagggtgaga atcagtcccg acctttcatc cgcaaggata tatatcagca ttttcccctc
      481 aaatgacaac aagcaggtta tgaaggcagt aaatgatcat attcccagaa taaggtatga
      541 gcttggccat cagataaaat cacagttgcg gattgtccct gaaattgcat ttttccttga
      601 cgacagtctc gattatattg agaatataga caatttgctg aaaccctgaa ctgaatgcag
      661 tacgagccaa tcaaaaggtc ggtcggcagg tttttctccg gctccctgtt catgagaaaa
      721 acactttatt ttctgctcga cattctcctt ctccggacat ggcacatcag gaaggcttta
      781 cgggaagcaa ggggcatcct gcctgacgat gcacggatac tggacgccgg ttcaggcttc
      841 gggcagtacg tgtggaggct gagccggatg gggagaaact ggaaaataac aggtgttgat
      901 attgacaggg aaatgattga ggactgcagg gattttttcg gcagggcagg cctgtcggaa
      961 agggtaagtt tccgggaagc cgatctcacc actttcagtg atcccgtgaa atacgacctg
     1021 atcctttcgg tggatgtcat ggaacatatt gaggatgacg ggagcgtttt cagaaacttc
     1081 cggcagtccc tcagcgaggg cgggatactg atcatatcga ctccttccga taccggaggc
     1141 agcggagtac acagccctga tgaggaatca ttcatagggg aacatgtaag gaacggatac
     1201 ggaacggatg aaatcagggg gaaactcaca gccgcaggct tcagcagcat caatataaaa
     1261 tacagttacg gcgtgccggg caatatttcc tggcatatat ccatgaaata cccggtaaga
     1321 ttactgaaca aatcaatggc cttcgccctt gtcctgccct tttactatct tattgcattt
     1381 cctgccgcac tgatacttaa ctttcttgat ctccgtttaa gtcatgaaaa aggcagcgga
     1441 ctggtcatta aagccgtaaa ataattgaaa ctggctctat acatagcaaa aagatatctt
     1501 ttcgcgaaaa aatcgcggaa tgccataaat gtcatttccg gggtgtcggt tgccggagtt
     1561 accgtcggca ccatggcact gataatcatt ttgtcggtat tcaacggact ggaactgctg
     1621 gtcactgcca ttttcaacac ctttgatccc gatctcaaga ttacggcagc ggaagggaaa
     1681 accttcattc ccgataccgc caggcttcac cgcctctccc gtatcgacgg ggtttcctgc
     1741 tactccctta ccctggaaga gaatgcatac ctcaaatacg gcgacaggga atatatcgga
     1801 acgatcaagg gtgttgacga aaactatgta agagttaccg gtatcgacag cacgatgtgg
     1861 gaaggggaat tcttactcac ttccgaaaat atgaggccct gggccgtagc cggactggga
     1921 gtggcaaatc atctgggaat gagagtaaat ttcgtaacac ccctcttcat ctatgtcccc
     1981 aggaaatctg caggaagcag catggattac gaaaatgcct ttatcaggaa atacatcttc
     2041 ccttcgggca tctttcagat agaacagacg tatgactcaa aatatgttta cctgcccctc
     2101 gatttcacca gggaactgat acaggatgaa gagggagtca gttccatcga aataaagcta
     2161 agcgcaaatg ccgatgcaaa cagggtccgt aaaaatgtaa acagtatctt cggggaagac
     2221 cttgtgatcc ggaaccgcta tgaacagcag gagatctttt acaaggtcat gcgttcggag
     2281 agactcgcga tattcatgat tcttaccctc ataatcatta ttgcctcgtt caatattgtc
     2341 ggatccctca ctatgctgat aatcgagaaa gagagggata tagagattct cagaagcctc
     2401 ggggccgaca ataagctgat ccggaagata ttcatctttg agggctggct tatctcgata
     2461 atcggaacag ctgcgggtgt gatactgggt ttcatcatct gctggataca gcaggaatac
     2521 ggagttgtca ggctgaacac ctcaaccctt atcatggaat cctatcccgt tgtgatgaaa
     2581 gccagggatt tcataatcat tcccgcgaca gtcctgctta taggtttctg ggcagcatgg
     2641 taccccgtca ggtttcttac caggaaatac cttggcaaac acgaaaaagg ttccgataca
     2701 tgatccgctg gatgaataaa atattgccgg ccattgcatt atgccttatc ttcccgtcat
     2761 gcaaaaacga ggaaaagaag ggactcattc ccctgaaaac cttttccagg atcatgtatg
     2821 aggtacatct ggctgacggc ctcctttcat catcagtgat ccgcaatcaa tactatgcaa
     2881 gggattccat tgccaattac cgggatattg ccgaaagtca cggctataca aaagaggccc
     2941 tggacaggac catgaaatat tatttcacaa aggaaccaaa gaagttcata aagatatatg
     3001 acaataccat cgccacgctg accgaaatgg agctgaggct ggaggaagag atggctgtaa
     3061 tgcctcccct ggccggcgga atgtggaaag ggcagcctga atatcatttc tcaggtcttc
     3121 cggataccaa caggatcagc tttgatcaca tatttcactc tccgggtatt tattcaataa
     3181 aatttaccct cacccttcat ccggccgacc agtctgtgaa tccatgcatg acaggctctt
     3241 tatgtcccgc cgacagtctt gccgacaggg agtgcgagtt tttcagagga ccggtatatc
     3301 tgaaggacgg acaggctcat acatatattt ttcccatacg tatatcaggc cggttgccgg
     3361 tacttttcag gggaaggctt cttgatttcg aaaacaggcc cggcgggcat ccgagatacg
     3421 gcacggttga aaatatctcg ttcacactta gttcttcagt actatgagac gtttgtcggc
     3481 cagccttgtc ttcacaaatg acggaccacc cctcaggaac ggcattgtaa caacaagcga
     3541 tgacggcacc atcctgaagg ttgaaaaggg cggaggttcc gtcccggagg ctgacccgga
     3601 tcgcgggtcc tcatccccgg agccggacac cgaattttac aacggaatca tcatacccgg
     3661 gttcgtaaac tgccactgcc atctcgaact gtcccatctg aaagggacaa tccctgcagg
     3721 caagggtctc ggtcccttcc tggcagatct taccgcgtca aggaagggag accctgaaac
     3781 gatccgcaag gcagcagctg ccgccgacag ggatatgtac acggaaggaa ccgaactgtg
     3841 tgccgacatc tgtaataact catcaacatt cggaataaaa agggaaagca gaatcagcta
     3901 cttcaacctt attgaagtgt ttgcatcaga tcccgccctt gccccgaaga gaatgcagga
     3961 agctgaaaac ctggcagctg agagtacagc ggcgggcctt cctttcagta tcgtgcctca
     4021 cagcgtttac agcgtgtcac ttccgctgtt caggctgata agggaaaaaa gcggcggaaa
     4081 cctggtgaat tcaatacatt tcatggaatc tgcaggagag gaaatgtatt tgtcggaccg
     4141 taaaggaccg ctgatggatt ccttttcaga ggccgggctg attcctcccg tactgcagac
     4201 accggaaaat tgtgtatcag ccattcttga tgaagtcaca acctccagat cgctcattct
     4261 ggtccataac accgtggccg tccgcgaaac ggtgaggaag ataaatccgc ggggaaaagt
     4321 gtactggtgc ctgtgcccca attccaatct ccatattgag ggaaaaatgc ccccggtttc
     4381 aatgctttcg gaggaaggct gcgatattgt tatcggcacg gacagcctgg cttcgaatga
     4441 aaacctgagc atactcgcgg aactgaaggc cattcagcaa cactatcctt cagcaaggct
     4501 tgaggatatg atccgctggt caacaatcaa cggtgcaaag gctctcggga aagaaagcag
     4561 gtacggcagt atcgctccgg gcaggaaacc cggacttctt cttattgaga atgctgacac
     4621 ggtaaatatg aaactgacac c
//
LOCUS       DAOSPX010000267         4640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16820_length_4640_cov_3.365758, whole genome shotgun sequence.
ACCESSION   DAOSPX010000267 DAOSPX010000000
VERSION     DAOSPX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16820_length_4640_cov_3.365758"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2084)
                     /locus_tag="PLZ75_11340"
     CDS             complement(<1..2084)
                     /locus_tag="PLZ75_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73027.1"
                     /translation="MKLSILPLLLITSFTLYSQEIIEPGEDIMGAFNAPGTECYELFI
                     EEAGEYLLSYNLMNVNIKVIYNGQELFSDYMAIFATDPKKAQIIFQNPGTYQICFSTT
                     GTMAVYQCRIVKADNIVPGEAIPVKYGEYVLKEISADDSPVNYSFSGKADDKIRIGIS
                     GMFVANLKITSPSKTVLYDQSFAAPPPNGLKLDFELEENGDYLITVTPGWGFQQYSLS
                     LYLLEPVQASFIKFNELYAGALANFGVNYLRFYACTGDTLQINYTGSTAYTTMQVITP
                     GGETVICKDIIDENHIAHTLPVEEDGTYVVVISYDMQGILGYGLSVEMKPEFYKLSRF
                     IIREMAHLYKKVYSFHASRNDILRIGLDLPSRILELKDPGGEIILRLDSTSSDNDKSL
                     SFNEITLESTGIYLITIWHDFTGYSPVEMRFCASLVPEPSVIEIDNMYHISVPPYHRY
                     PYSFNAEADHVIRIILPEASPMVTPAGDVITVNTGKTHMVQQSGNFTISAINSLTVTA
                     ETDILLKTIVPPENAVPLEIGKIFKDNIELYETPSIVYNGIAGDTVFVQAEIKELILS
                     PNVGNIINTPSWLYVTRPSGGLLRIEGKPTGRISDNGYLITYSFKKNIILDQSGNHFM
                     TLCMDKEVTENTVDVTMRIDRGGASIEVPFGTYSDYEVPGNYYCSVPQGLEKLFVILK
                     RSNNIGYDQTWHG"
     gene            complement(2096..2461)
                     /locus_tag="PLZ75_11345"
     CDS             complement(2096..2461)
                     /locus_tag="PLZ75_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73028.1"
                     /translation="MKVMGTGSHQETTKIQTNVPVDAKYFVIPKNIEFSDMPMLNQRW
                     EYYRPLDNNLSSFNASLISIQYDYSNSRPGFGHEATTIRKKSPLKRQIPEKEYLLRSN
                     TDRTEMTFNWQVTRKKKPL"
     gene            complement(2539..4296)
                     /locus_tag="PLZ75_11350"
     CDS             complement(2539..4296)
                     /locus_tag="PLZ75_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73029.1"
                     /translation="MPKEQEHDFSGFIAGDWNSRILDLGAAYLDECPLKIEILSHSGV
                     RDLNEMVEGITAVTTGKPKRNPSEYQDPKMNVDYIWKGILELTYIEKIVPGSIEPAYE
                     GGTEYIPGYVEGGWKFTIQLYDVQHDEIVKEAHTTWTGTPLGFQKNLKNEYFAGKTEK
                     IRIEILKELFNREFKDLKKIIIDYEKAPKKAEFESEVIKVESDKTKRITFKVTDEKGE
                     KPKKWQRLAVKVDHGSLTNGTRCCDFSEGPKFYSFLCENGNVTIEYKAPPEEGSRLDH
                     ITVFNGCVTRDPPVIRMEMVDEREEIGSVDIDINPVYETRLIVKGSFINNASYRRNFK
                     DEKGTERYREDLNEVQEATFYVPLELERSDDMPILNQVWEYYRPKSITLTNCNIYSRK
                     YTSSYASSPAGGHDLKSITIKEPLYLEIFGKEAALQSNILVIIDKETGKVVKASPGGF
                     AVDFFWHERYQQKGKVWSKEEGEKPVNVSENKTDEYRSEYSPGPVEDPVPDPTFRSIS
                     QSLRTFLNNMGTPLPANIEIPEDEEHPEIEPELLVKFGDGKKFFGGKGKVVLENEYGP
                     DSRRYSEKTFYWQITRTRK"
BASE COUNT         1253 a          989 c          898 g         1500 t
ORIGIN      
        1 ccatgccacg tttggtcata tccaatatta ttactccttt tcaggatcac aaaaagcttt
       61 tcgagccctt gaggtacaga acaataataa ttaccgggta cttcatagtc ggagtatgtc
      121 ccgaaaggaa cttcgattga tgcacctccc cggtcgatgc gcatagtgac atccacagta
      181 ttttcagtga cctccttatc catgcagaga gtcatgaaat ggttgccgga ctgatcaaga
      241 ataatattct ttttgaaaga ataggttatc agatatccgt tgtcagaaat tcttccagtc
      301 ggttttccct caatccttaa caatccgccc gaaggtctgg tgacatataa ccagcttggt
      361 gtattgataa tgttgccgac attaggggac aaaatcaact ctttgatttc ggcctgtaca
      421 aaaacagtgt cgcctgcaat tccattatat actatagatg gcgtttcgta cagttcaata
      481 ttatctttaa aaattttacc aatttcaagc gggacggcat tttcaggagg aacaatggtt
      541 ttcaacagga tatcggtctc agccgtaacg gttaaagaat taatggcact tattgtaaaa
      601 ttgcctgatt gctgtaccat atgagtttta ccggtgttaa cggtaattac atcgcctgct
      661 ggtgttacca ttggcgaagc ttcaggaaga ataatcctga taacatgatc ggcttcagca
      721 ttaaaagaat aggggtatct atgataggga ggaacagaaa tatggtacat attatctatt
      781 tctatcactg aaggttccgg gacaagagaa gcgcaaaacc tcatctcaac agggctatac
      841 cctgtaaaat catgccagat ggtaattaaa taaataccgg tgctttccag tgttatttca
      901 ttgaaagaaa gtgatttatc attgtctgaa gaagtgctgt ccagcctgag gattatttct
      961 ccccccggat cttttaactc cagaatcctg gatggaagat ccaggccgat ccttaaaata
     1021 tcattccgag aggcatgaaa tgagtaaact tttttataca gatgagccat ttccctgatg
     1081 atgaatctac tcagtttata aaattcgggt ttcatttcaa cactcagtcc ataccctaaa
     1141 ataccctgca tgtcatagct gatgaccacc acatatgttc catcctcttc aacagggagg
     1201 gtatgagcaa tatggttttc atcgattatg tccttgcaga ttacagtttc gcctcccggt
     1261 gttataactt gcatggttgt gtatgctgtt gaacctgtat aattgatctg cagtgtatct
     1321 cctgtacagg cataaaaccg taaataattc acaccgaagt tggccagtgc accagcgtac
     1381 agttcattga atttaataaa tgaagcctga acaggttcaa gcaggtaaag agaaagggaa
     1441 tattgctgaa agccccatcc cggagtaacc gtgatcaggt agtcgccgtt ttcctccagt
     1501 tcaaaatcca gtttgagtcc atttggggga ggcgcagcaa atgactgatc atacaacacg
     1561 gttttcgacg ggcttgtaat ttttaaatta gccacaaaca ttcccgaaat gccaatccgg
     1621 atcttatcat cggctttccc gctgaaagag taattgacag gggaatcatc cgcacttatt
     1681 tccttaagaa catactcacc gtatttaaca gggattgcct ccccgggtac tatattatcc
     1741 gccttgacaa tccggcactg gtacacagcc attgttccgg tggtggaaaa acaaatctgg
     1801 tatgtacccg ggttttggaa tattatttgc gccttcttcg ggtcagttgc aaagatggcc
     1861 atataatcgc tgaatagttc ctgcccgttg taaataactt taatgttgac attcatgaga
     1921 ttatagctca gcaggtattc tccggcctct tcaatgaaga gttcatagca ttccgtaccc
     1981 ggagcattga atgcacccat aatatcctcc cctggttcga taatttcctg gctgtataaa
     2041 gtaaaagatg ttattaaaag caggggaaga attgacagtt tcatatatat atgatttata
     2101 atggtttctt ttttcttgtt acctgccagt taaaggtcat ttctgtacga tcagtatttg
     2161 atctgagtaa atattctttt tcgggaatct gtcttttcag tggacttttt tttctgattg
     2221 ttgtcgcttc atggccaaaa cctggtcgac tatttgaata atcatattgt atggatatta
     2281 atgaagcatt gaatgaagac agattattat caagaggccg gtaatattcc catcgctggt
     2341 taagcatcgg catgtcggag aattcgatat tctttggaat gacaaaatat ttggcatcaa
     2401 cgggaacatt ggtctggatc tttgttgtct cctgatggga tcctgttccc attaccttca
     2461 tatccagaag cataattaat aagctgattg taaatgccac cattatttat ataatcttat
     2521 tgttttttgt ttactcgttt atttcctagt gcgtgttatt tgccagtaga aggttttttc
     2581 ggaatatcgc ctggagtcag gtccgtattc attttcgagg actactttac cttttcctcc
     2641 aaaaaatttt ttaccatcac cgaatttaac cagcagctcg ggttctattt ccgggtgttc
     2701 ttcatcttca ggtatctcta tattagcagg caggggagta cccatgttat ttaagaatgt
     2761 cctgagagac tgtgatatac tcctgaacgt cggatcagga accgggtcct caacaggtcc
     2821 gggagaatac tcggaacgat attcatcggt cttgttttct gacacattga caggtttttc
     2881 tccttcttcc ttggaccata cttttccttt ctgctggtac ctttcatgcc agaaaaaatc
     2941 aacggcaaaa cctcctggtg aagcctttac caccttgccg gtttccttat caataattac
     3001 caggatattt gattgcagtg cagcttcctt tccgaaaatt tcaaggtaca gaggttcttt
     3061 gatggttata cttttcaaat catgtcctcc cgccggggat gatgcataac tactggtata
     3121 tttccgggaa taaatattac agttggtaag ggtaatgctt tttggccggt aatattccca
     3181 tacctggttt aatatgggca tatcatcaga ccgttcaagc tcaagaggaa cataaaaagt
     3241 agcttcctgt acttcattca ggtcctctct gtatctctca gttccctttt catctttaaa
     3301 attacgcctg taacttgcat tgttgataaa actgcctttt acaatcaggc gggtctcata
     3361 taccggattt atatctatat ccactgatcc gatttcttca cgttcatcaa ccatttccat
     3421 tcggatgaca ggaggatcac gggtcacgca tccattaaat acagttatat gatcaagtct
     3481 tgatccctct tccggagggg ccttatattc aatagtcacg ttgccgtttt cgcacaaaaa
     3541 agaataaaac ttaggcccct cgctaaaatc acaacacctt gtcccatttg tcagagagcc
     3601 atggtcaact ttaactgcca gtctttgcca tttctttggt ttttctccct tttcatctgt
     3661 aactttgaag gtgatccttt ttgttttatc agattcgacc ttaattactt cgctttcaaa
     3721 ttcagctttt tttggagctt tctcgtagtc gatgatgatt ttcttcagat ctttgaattc
     3781 tctgttaaaa agctctttca gaatctcaat ccggattttc tctgtttttc cggcaaaata
     3841 ttcattcttc aaatttttct gaaatcctaa tggtgttcct gtccaggttg tgtgtgcctc
     3901 ttttactatc tcatcgtgct ggacgtcgta taactgaatg gtgaatttcc atccaccctc
     3961 aacataaccc gggatatatt cagttcctcc ctcatatgca ggttctatcg agccgggtac
     4021 tattttttca atatatgtaa gttcaaggat acccttccag atgtaatcaa cattcatttt
     4081 cggatcttga tattctgaag gattccgctt tggcttgcct gttgtcacgg cagttatgcc
     4141 ttcgaccatt tcattaaggt ctcttacccc ggagtgactt agaatttcaa tctttagagg
     4201 acattcatcc agatatgccg cacctaagtc aagaatacgc gaattccagt caccagcaat
     4261 aaacccgcta aaatcgtgtt cctgttcttt aggcatctta aaaggcggga acattatggt
     4321 tctccagcaa caatattctt cctgtgctga tccggataat gagattacac ccaatagcaa
     4381 tatcaggcat ctttttagta atttgcggat tgtaattctt ttcatataat tagataatca
     4441 gagtatttcc acgttacaaa ttatcagaag cagtcatccg ggatagtcag ctggtatact
     4501 tccgccatca gcgcctatta ctgagctttc acggatgtcg gggaagtaag ctgcagcgat
     4561 cagcaaggga aatattaatg tctgcaggga tataagaaga aatcgcttcc ttaatgttcc
     4621 ggtgttcata acaggagtta
//
LOCUS       DAOSPX010000268         4619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_16912_length_4619_cov_3.481595, whole genome shotgun sequence.
ACCESSION   DAOSPX010000268 DAOSPX010000000
VERSION     DAOSPX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16912_length_4619_cov_3.481595"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLZ75_11355"
     CDS             <1..594
                     /locus_tag="PLZ75_11355"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOS73030.1"
                     /translation="TGQETGSTLREVDLYTGRVNRQLNLSPELFGEGITLYRDRIYQL
                     TWRSRVGFVYEKSTFRQINKIYYQSEGWGLTTMDDRIVMSDGTNVLYIIEPEMFTVIS
                     RLEVYDNNGMVDQLNELEYINGEIWANIWNTDLIARIDPATGKLLAYIDLKGILRDPG
                     TDTSIDVLNGIAWDSDGGRIFVTGKNWPSLFEIRVTE"
     gene            complement(657..1610)
                     /locus_tag="PLZ75_11360"
     CDS             complement(657..1610)
                     /locus_tag="PLZ75_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOS73031.1"
                     /translation="MSGYSTRKEWFVIVNPNAGGGKGKKDWKRISDLLTRENISFISR
                     FTGRRGQAIDYTDEAIEKGFRRFISVGGDGTLNEVVNGIFIRNRVPANEITIAMIPVG
                     TGNDWGRMFGIPSIYEGAVKVIAEGKTMLHDIGLVDYFEGEQSRRRYFINITGLGFEA
                     LVVKKTNRQKDRGRNSKAIYFFNILSSLVSYRITAADIIIDGRKSSADIFSINVGNGR
                     YCGGGMRQTPDALPDDGLLDVTVIKEMGRLEIIKNLRLLYDGTILSHPEVDGYRCRNL
                     KIESESLLFIESDGELLGHTPAEIGIIPSAVNIVYAGRLFP"
     gene            1995..2780
                     /locus_tag="PLZ75_11365"
     CDS             1995..2780
                     /locus_tag="PLZ75_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS73032.1"
                     /translation="MGRKIAIANQKGGVGKTTTAINLAASLAALEKKVLIIDADPQAN
                     ATSGIGLDTRQMRSTIYDCLIEGKNPEEAVLGSEIENLFLIPSNIDLVGAEIEMLDKP
                     DREKILRNVIAGIEDRYDFIFIDCSPSLGLLTVNALTAANSVIIPVQCEYFALEGLGK
                     LLNTIKIIQANLNTGLEIEGFLLTMYDPRLRLSNQVADEVNKHFQQMVFNTIIQRNVK
                     LSEAPSFGQPAILYDAESRGAVNYLSLAKEILEKQEARLKLVQ"
     gene            2809..3690
                     /locus_tag="PLZ75_11370"
     CDS             2809..3690
                     /locus_tag="PLZ75_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOS73033.1"
                     /translation="MAKKNALGRGLGALIEGVEKEVLEKIVEANKDIPVDLVDANPFQ
                     PRTSFDEQAMEELAASIRKLGIVQPLTVRESGDGRFQLIAGERRLRAAKLAGLTHVPA
                     YIRTADDQAMLELALVENIQREDLDAVEVAISFQRLIEECRLTQEQLSERVGKQRSTV
                     ANYLRLLKLPAEIQLGIRNKNLTMGHARALVNIGDPRQQIDIYYKVVDGELSVRQTEE
                     LVRQLQAKAGKDPEKIRRKQQLNEDFVQLTNHLNRIFSVNVNFRINEEGKGKIVIPFG
                     NSEEMERILGVFDRLNS"
     gene            3743..4378
                     /locus_tag="PLZ75_11375"
     CDS             3743..4378
                     /locus_tag="PLZ75_11375"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOS73034.1"
                     /translation="MKAQHYIIFFIVLLLNCSVSNVSAQDSLFIETREKKMFRADPLR
                     ATMLAVALPGLGQIYNRKYWKIPLVYAGFGGVLYAINYNSTNYNVFMKAYQDFTDKIP
                     ETDSYLKLIRNADPATYDPVLHPDTYDPSAASWYTDRMLRQVDYFKKYRDLSYIAFAA
                     WYLITVLDANVDASLLNYDISNNLVLQMLPVPSLHPAYAGPGMSVGLCFSF"
     gene            4392..>4619
                     /locus_tag="PLZ75_11380"
     CDS             4392..>4619
                     /locus_tag="PLZ75_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73035.1"
                     /translation="MMRIKLFVAVFSLMVLVSNTLSAVGDTIIIRSDISTEKISADLD
                     SLVNSWYVRTALASWSELPPGDAGEEIMFSDS"
BASE COUNT         1280 a          986 c         1132 g         1221 t
ORIGIN      
        1 acaggacagg aaacaggatc aacactcagg gaggttgatc tgtatacggg cagggtaaac
       61 cgccagctca acctgagccc ggagctcttc ggtgaaggca taaccctgta cagggacaga
      121 atttaccagc tgacatggag atcaagggtg ggatttgtct atgaaaaatc cactttcagg
      181 cagatcaaca agatatatta tcagtcggag ggctggggac tgacgaccat ggatgacagg
      241 atcgtgatga gcgacggtac caatgtgctg tacattattg aaccggaaat gttcacagtg
      301 atctcacggc ttgaggttta cgacaacaat ggaatggttg atcagctgaa tgagctggaa
      361 tacattaacg gtgagatctg ggcaaatatc tggaacaccg atcttatcgc gaggatagat
      421 ccggctaccg gaaaactgct tgcctatatc gacctgaagg ggatactcag ggatcccgga
      481 accgatacca gtatagatgt tcttaacgga atagcatggg acagtgacgg cggaaggatc
      541 tttgtaacgg gaaagaactg gcccagcctc ttcgaaataa gagtcacgga ataaactttc
      601 ccgcatgaat aatatacagc aggatcgccg tgaacgtttg tcagtgtccg taattgtcag
      661 ggaaacagtc ttcctgcata aacaatattc actgctgacg gaataatacc gatctccgcg
      721 ggtgtatgtc ccagcagttc cccgtctgat tcaataaaga gcaatgattc gctttcaatt
      781 ttcaggttcc tgcacctgta accgtcaact tccggatggc tcagtatcgt accgtcatac
      841 agcagcctga ggttttttat tatttccagt cttcccattt cctttatgac tgtcacatca
      901 agaagtccgt catcaggcag tgcatcgggg gtctgtctca ttcccccgcc gcagtacctg
      961 ccgttgccga cattgattga aaaaatgtct gctgagctct ttctgccgtc aataatgata
     1021 tcagcggcgg ttatcctgta ggaaaccagg cttgagagaa tattgaagaa atagatggcc
     1081 ttgctgttac ggcccctgtc cttctgccgg tttgtttttt ttacgaccag agcctcgaac
     1141 cccagtccgg ttatatttat gaaatatctt ctgcggctct gctccccctc gaaatagtca
     1201 acaagcccta tgtcgtgcag catggttttt ccttcagcta tcactttcac agccccctca
     1261 tagatactgg gtatcccgaa catccgtccc cagtcattgc ctgttccgac cggaatcatg
     1321 gcgattgtaa tttcgtttgc cggaaccctg ttccggataa agatcccgtt taccacttca
     1381 ttcagggtgc cgtcgcctcc cacggaaata aagcgcctga atcctttttc aatggcttcg
     1441 tcagtgtagt cgattgcctg tcctcttctt ccggtgaacc gtgaaatgaa agaaatgttt
     1501 tcccttgtca gaaggtctga aatcctcttc cagtcctttt ttcccttgcc gcctccggca
     1561 ttgggattga caatgacgaa ccattctttc cgggtgctgt agcctgacat gattattaaa
     1621 atttaaacct aaagataaat tattaaagac aaaggggagg gtgggaaaaa tacttttctt
     1681 cccgtgaaac tgttatttat aatcaggggc gggaacagtc acggtgaaat ttgataatat
     1741 tccgggaaat cattattatt atcatatatt aggatatttc cagaataatg attatttggg
     1801 aacacttgct ttaatattgt aaaatacaaa taatcagata aatataagac tgctttgtat
     1861 ctttgcattg ttaataaaat gttgaaaacc ccggataaaa tgtttgtaat tagatacggt
     1921 ggtttccgga ctgctggtcc tttaatagta aatttgaagc tcaaaatata agtacaaaac
     1981 cttaaggaaa acagatggga aggaaaattg caattgcgaa tcagaagggt ggtgttggaa
     2041 agacaacaac tgcgataaac ctggctgcga gcctggcagc gcttgaaaag aaagtactga
     2101 taatagatgc ggatccccag gcgaatgcca cttcaggcat tggtctggac acacgtcaga
     2161 tgagaagtac catatatgac tgtcttattg aggggaagaa tccggaagaa gccgttctgg
     2221 gatctgagat tgaaaacctc tttttgatac cgtcaaacat tgaccttgtg ggggcagaga
     2281 ttgagatgct ggacaagccc gaccgggaaa agatactgag gaatgtgata gccgggattg
     2341 aagacaggta tgatttcata ttcattgatt gttcaccctc tctggggttg ctgactgtga
     2401 atgctctcac cgcggccaat tcggtgatta ttccggttca gtgtgaatat ttcgcacttg
     2461 agggactggg caagcttctc aatacaatca agattattca ggccaacctg aataccggcc
     2521 tggaaataga aggttttctt cttaccatgt atgatcccag gctgaggttg tccaaccagg
     2581 tagccgatga ggtgaacaag catttccagc agatggtgtt caacacgatt atccagagaa
     2641 atgttaagct ttccgaagca cccagttttg gtcagccggc aattctctat gatgctgaat
     2701 ccaggggagc agtcaattat ctgagtctgg caaaggagat cctggagaag caggaagcca
     2761 ggcttaaact ggtgcagtga atgtgcttat tttttaaaca gtggagatat ggcaaaaaag
     2821 aatgcattgg ggagaggatt gggtgcgctg attgagggag tcgaaaagga agtcctcgag
     2881 aagatcgttg aagccaacaa ggatattccc gtggatctgg ttgacgcaaa cccgtttcag
     2941 ccaagaacca gctttgatga acaggcaatg gaggagcttg ctgcgtcaat ccgcaagctt
     3001 ggaattgttc agcctcttac cgtacgggag tcaggtgacg ggcgctttca gctcattgca
     3061 ggagaaaggc ggctcagggc tgcaaaactg gccggactga cacatgtgcc ggcatatatc
     3121 agaacagctg atgaccaggc catgcttgaa ctggcgcttg tggaaaatat ccagcgtgag
     3181 gatcttgatg cagttgaagt ggcaatcagc tttcagcgcc tgatagaaga atgcaggctt
     3241 acccaggaac agcttagcga gagggttggc aagcagagat caaccgtggc aaattatctg
     3301 cggctgctga agcttcccgc tgaaatccag cttggtatcc gcaacaagaa cctgacaatg
     3361 gggcatgcaa gggcactggt aaatatcggg gatcccaggc agcagattga tatctattac
     3421 aaggtggtgg acggagagct ttctgtaagg cagacggagg aactggtacg gcagcttcag
     3481 gcaaaagccg gaaaggatcc ggaaaagatc agaaggaaac agcagctcaa cgaggatttt
     3541 gtacagctta caaatcatct aaacaggata ttttcagtta atgttaattt cagaatcaat
     3601 gaggagggaa aggggaaaat agttatccct ttcggcaaca gtgaagagat ggaacgcatt
     3661 ttaggggttt tcgaccgcct taacagctga aaataattat ctttattgcg ccgttttact
     3721 gatacttatg cttctctgtc aaatgaaggc gcaacattat ataatctttt ttattgtttt
     3781 actgctgaat tgttctgtaa gtaacgtatc tgcacaggac agccttttca ttgaaaccag
     3841 ggaaaagaaa atgttcaggg ccgatccgct gagggccaca atgcttgcgg ttgcattgcc
     3901 cggactgggt cagatctata acaggaaata ctggaaaatt ccccttgtat acgccgggtt
     3961 cggaggagta ttatatgcca ttaactataa ttcaacgaat tacaatgtgt tcatgaaggc
     4021 ataccaggat tttaccgata aaatacccga gacagactcc tatctcaaac tgatcaggaa
     4081 tgcggatccc gcaacctacg atcctgtcct tcatcctgac acctatgatc cttctgccgc
     4141 atcctggtac actgacagaa tgctgaggca ggttgattat ttcaagaaat accgtgatct
     4201 ttcatatatt gcatttgcag cctggtacct tataacggta ctggatgcca atgtggatgc
     4261 aagcctgctt aattatgaca taagcaataa tcttgtgctg cagatgcttc ccgttccgtc
     4321 cttgcatccg gcttatgccg gaccgggcat gagcgtgggc ctgtgtttta gtttttgatt
     4381 aaagataagg aatgatgaga ataaagttat ttgttgcagt tttctccctg atggtattgg
     4441 taagcaatac cctgtcggct gttggtgata cgataataat acggtccgac atttccactg
     4501 aaaaaatctc cgcggatctt gacagcctgg ttaattcctg gtatgtcaga actgccctgg
     4561 catcctggtc tgaattgccg cccggcgatg caggggagga aatcatgttt tccgacagt
//
LOCUS       DAOSPX010000269         4594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_17040_length_4594_cov_3.380921, whole genome shotgun sequence.
ACCESSION   DAOSPX010000269 DAOSPX010000000
VERSION     DAOSPX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17040_length_4594_cov_3.380921"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PLZ75_11385"
     CDS             complement(<1..1293)
                     /locus_tag="PLZ75_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS73036.1"
                     /translation="MKEEIISCSAGLLILTGAVAKAQEAPKENPNIIFLLVDDLGWRD
                     VGFNGSCFYETPNLDRLAARSMVFNNAYAACAVSSPTRASLQTGRYPARIGITDWIRA
                     KFQVKDGIIETPPPYEENPDRKLKTPSNPYWMDLEEITTAELLKTRGYFTCHIGKWHL
                     GPDDYYPEKQGYDLNIGGSDMGQPVNYFDPYDRDGVSFPNIKPRREGEHLEDRLSDEL
                     VQVIRQHKDRPFFINMCYYSVHTPLMAKPETVDRYEAKNRTDDQTNAVYAAMIEHLDD
                     AVGKLVNTLEEEALMDNTIIIFFSDNGGLVGPTSNSPLRSGKGYPYEGGIKEPMFVYW
                     KNVIQPGTKCDVPVCSIDFLPTLCAITGAALPDRIIDGRDISPLFSGRKMKQVPLYWH
                     FPHYRVGDIVPYSIIRDGNWKLIKWYEGRSFELYNLRKD"
     gene            complement(1412..2650)
                     /locus_tag="PLZ75_11390"
     CDS             complement(1412..2650)
                     /locus_tag="PLZ75_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS73037.1"
                     /translation="MNKANNSVLDRLGMPAQLGWGYLGILIFMTGNGIELGWLSPYLV
                     DRGMTIEQSALLFTVYGIIIAISSWFSGVLAESAGPKKTMLTGLILYIAATIGFVGFG
                     VRDLNFPVMLVTYALRGFGYPLFAYSFLVWIAYVSPQEKLGRAVGWFWFIFTGGLNVL
                     GTYYSIWAIKNLGNINTLWTSVIWVLLGGLFAIILNRAPFPARDKDKQENKLKELLKG
                     LTIVKKEPKVLLGGIVRIINTTAQFAFPVFLPIYLYGHGFSTADWLKIWGTIFTANIL
                     FNLIFGFVGDRLGWRNTIIWFGCIGCMITTLLFFYSAQLSGGSFWIVLICGILWGAAL
                     SGYVPLSALVPTLVKEDKGAAIAILNLGAGLSVFAGPAIVGLFIGLIGNEGVIWILAA
                     LYLLSAILTKFITLPGNTSG"
     gene            2801..3316
                     /locus_tag="PLZ75_11395"
     CDS             2801..3316
                     /locus_tag="PLZ75_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73038.1"
                     /translation="MRNLTLLTILTILVTAGGGCSNNKSSGNRLKEQAVASAREHILE
                     ITRNGDVTVDKEGIITITAGRISYLIDPSAVLIEEINEDPLKDAIVPVTGFDDGGMLA
                     KKHLIMINSDNAMLLDTVLTDVVRILKVEDGVIYAELSKVSMDSPYYGCAECVEIASY
                     RFREGKLEKIE"
     gene            complement(3498..>4594)
                     /locus_tag="PLZ75_11400"
     CDS             complement(3498..>4594)
                     /locus_tag="PLZ75_11400"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS73039.1"
                     /translation="PDLKWNEAKKELDDIISSGKYELMPNFDDNFLAAKRNNKESVFE
                     IQYSVNDGASGSPNAGWGQSLNFPQDVDGMGTCCGFFQPTQNLVNAFIVDPVTGLPFL
                     DADTLANFKNDMNVASNEFFAQDTVTPVDPRLDWTVGRRGVPYLDWGVMRGKDWIRDQ
                     ANGGPYVYKKNMFMKKDKGTLSTTTGWATGVNSNSYRAIRYSHILLWRAEVAVESSPA
                     DLEYAAQLVNMIRQRAGNHVLMGRCRMFQMATTQDIIAPKIDWSVPAANYLVNPYPVP
                     FTSPEQARKAIRHEIRLEFAMEGHRFFDLVRWGIAGKTLNMYITQDRNFRSLLGGVKP
                     AVFENKNEYWPLPQTQLDLQPGVLTQNPGY"
BASE COUNT         1235 a         1050 c         1041 g         1268 t
ORIGIN      
        1 atccttcctg aggttgtaaa gttcaaagga ccggccttca taccacttga tcagtttcca
       61 gttaccgtca cgtataatgc tgtagggcac gatgtcgccc acccggtagt gagggaaatg
      121 ccagtagagg ggcacctgtt tcattttccg gccggaaaag agcggactta tatcgcggcc
      181 gtcaatgatt ctgtcaggaa gagcagcacc ggtaatcgca catagtgtcg gcagaaaatc
      241 gatactgcat acgggaacat cgcattttgt accaggttga atcacgtttt tccagtatac
      301 aaacatgggt tcctttattc ctccctcgta tggatatccc tttcccgaac gcagggggct
      361 gttgcttgtc gggcccacta gtcctccgtt gtcagagaag aagattatta ttgtattgtc
      421 catcagtgct tcttcctcca gggtgttaac cagtttcccg acagcgtcat ccagatgttc
      481 aatcatagcg gcataaacag cattggtctg gtcatcagta cggtttttgg cttcgtacct
      541 gtcaaccgtt tccggttttg ccatcagggg cgtatgaaca gaatagtagc acatgttaat
      601 aaaaaagggc cggtctttgt gttgcctgat tacctgaacc agttcgtcag aaagccggtc
      661 ttcaaggtgt tccccctccc tgcggggttt gatattcgga aagctgacac catccctgtc
      721 gtaaggatca aaataattga ccggctggcc catatcgctt cctcctatat taaggtcata
      781 accctgtttt tccggataat aatcatccgg tccgagatgc catttcccta tatgacaggt
      841 aaaatacccc cttgttttca gtaattccgc tgttgtaatt tcttcaagat ccatccagta
      901 gggattcgag ggagttttca gcttcctgtc ggggttctcc tcatagggcg ggggagtttc
      961 tattatcccg tccttaacct ggaattttgc acggatccag tcagttattc ctatacgtgc
     1021 aggatatctt cccgtctgga gggatgcacg tgtaggggag gatacggcgc aggctgcata
     1081 ggcattattg aaaaccatac ttctggcggc aagtctgtcg agatttggtg tttcataaaa
     1141 gcagctgccg ttgaaaccca catccctcca gccaagatca tcgaccagga gaaaaataat
     1201 attcggattt tccttcggag cctcctgtgc ttttgcaaca gctcctgtaa gaataagtaa
     1261 tccggcagaa caggaaatga tttcttcttt catggtttaa aataataagg ctttgttaaa
     1321 ataatccatt aatatgaaag cgcaaaagga aatttacaat tgctggctga agagaaaaaa
     1381 cacagccagg cagtacagta agttgttcaa tttatcctga ggtatttccg ggaagtgtta
     1441 tgaattttgt aagtattgca cttaagagat agagtgctgc aagaatccat ataacaccct
     1501 cattccctat aaggccgata aagagtccta cgatagcagg accagcaaat accgataatc
     1561 ctgcccccag attaagaatt gctattgctg ctcccttgtc ttcctttacc agtgtgggta
     1621 ccagtgcgga aaggggtaca tagcctgaca gggctgctcc ccaaaggatg ccgcaaatga
     1681 gtacaatcca gaagcttccg cccgaaagct gtgctgaata gaaaaacagc agtgttgtga
     1741 tcatacatcc gatgcatccg aaccatatga ttgtattcct ccagccgagc ctgtctccga
     1801 caaatccgaa tatcaggttg aacagaatat ttgctgtaaa tattgttccc cagattttca
     1861 gccagtcagc cgtggaaaat ccatgtccgt aaaggtatat aggaaggaaa accggaaaag
     1921 caaactgggc tgtggtattg atgatcctca ctattccgcc gagaagaact ttcggttctt
     1981 tttttacaat tgtcagccct ttcaggagtt cctttaattt attttcctgt ttatccttat
     2041 cccgggcagg aaagggggcg cggttaagaa ttatagcaaa taatcctccg agcaggaccc
     2101 agatgacaga agtccataga gtattgatat ttcccagatt cttaattgcc catattgaat
     2161 agtaggtccc gagcacgttc aatcctcccg taaatataaa ccagaaccat cctactgccc
     2221 tgcccagctt ttcctgcgga ctaacatagg ctatccaaac caggaaggag taggcgaaaa
     2281 gtggatagcc gaaaccgcgc agggcatagg taacaagcat gacagggaaa ttcaggtccc
     2341 tgaccccaaa gccgacaaaa cctattgttg cggcaatata cagaatcagt cccgtcagca
     2401 ttgttttttt cggaccggca ctttcggcca gtacacctga gaaccaggac gagattgcga
     2461 taatgatacc gtaaacggta aacagcaggg cagactgttc aatggtcatt cccctgtcaa
     2521 caaggtaggg actgagccat cccagttcaa tgccgttacc cgtcatgaag atcagaatgc
     2581 ccagatagcc ccagcctaac tgagcaggca ttcccaatct gtcaagaaca gaattatttg
     2641 ctttattcat ttttgtgaaa cagatttttt gttttaggca aacaaggtac aaaatagtat
     2701 gatatcaatt tggcattttt ctgaatatcc ctgtgaccac aaaacgtgat tttcataaat
     2761 ttcattttta ttatacattt gaaggttaat gctcaaaatc atgcgaaatc taacattact
     2821 gactattctg acaattcttg ttacagccgg tggcgggtgc agcaacaata aatcatccgg
     2881 gaacagactg aaggaacagg ccgttgcatc tgcaagggaa catattcttg aaataacccg
     2941 caatggtgat gttacagttg ataaggaggg cattattaca attaccgccg gacggatcag
     3001 ttatctgatt gatccttcag ccgtgctgat tgaagaaata aatgaagacc cgctcaaaga
     3061 tgccatagtg cccgttaccg gttttgatga cggcggaatg cttgcaaaaa agcatctcat
     3121 aatgatcaac tctgacaatg ccatgctgct tgacactgtt ttaaccgatg ttgtgagaat
     3181 tctgaaagtg gaagacgggg ttatttatgc tgaattatcg aaagtttcca tggattctcc
     3241 gtattacggc tgtgcagaat gtgttgaaat tgccagttac cggttcaggg agggaaaact
     3301 ggaaaaaatt gaataaggaa agaaagagat ttcaggtata aaactgaaaa tcagaatcaa
     3361 agccgtatgc cggaggttaa ggaaagcctc gtaagatgta accggataag agattaacag
     3421 aaatttctcc ggaaaaaatc agaggcagcc tcaatgggct gcctcttcta ttttaccaca
     3481 tattattctg tctgttttta gtaccccggg ttttgtgtaa gtacacccgg ctgcaggtca
     3541 agctgagtct ggggaagcgg ccagtattca tttttgtttt cgaacacggc aggcttgact
     3601 cctccaagca atgaccggaa atttctgtcc tgtgttatat acatgttgag tgtcttgcct
     3661 gcaatacccc agcgaaccag atcaaagaac ctgtgtcctt ccattgcaaa ctcaagtctt
     3721 atttcatggc gtattgcctt ccgggcctgt tcagggcttg taaagggaac aggataggga
     3781 tttaccaggt aatttgctgc cggtacgctc cagtcaattt ttggtgcaat tatatcctgg
     3841 gtggttgcca tctggaacat tctgcatctg cccatcaata catgattgcc tgctctttgg
     3901 cgtatcatat tgaccagttg tgctgcatat tccaggtcgg ccggggaaga ttctacggcc
     3961 acctctgctc tccagagcag gatatgggaa taccttatgg ccctgtagct gtttgagttc
     4021 actccggttg cccaacccgt ggttgtggat agagttccct tgtccttttt catgaacata
     4081 ttctttttgt aaacataggg acctccgttt gcctggtcgc ggatccaatc cttgccgcgc
     4141 atgacacccc agtcgagata gggcacacct cttcgtccca cggtccagtc cagtctggga
     4201 tcaacgggag taaccgtatc ctgggcaaaa aattcattgg aagccacatt catgtcattc
     4261 ttgaagtttg caagagtgtc ggcatccagg aatggcaaac cggttacggg atcaactatg
     4321 aaggcattca ccaggttttg tgtgggctga aagaatccgc agcaggttcc catgccgtcc
     4381 acgtcctgtg gaaagttgag agattgcccc catccggcat tgggtgaacc gcttgcacca
     4441 tcatttacgc tgtactggat ctcaaatacg gactccttat tatttctttt ggcagccagg
     4501 aagttatcat caaaattagg catgagttca tatttcccgc ttgaaataat atcgtccagc
     4561 tcttttttag cttcgttcca ttttagatct ggat
//
LOCUS       DAOSPX010000270         4583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_17095_length_4583_cov_3.628754, whole genome shotgun sequence.
ACCESSION   DAOSPX010000270 DAOSPX010000000
VERSION     DAOSPX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17095_length_4583_cov_3.628754"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PLZ75_11405"
     CDS             complement(<1..919)
                     /locus_tag="PLZ75_11405"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOS73040.1"
                     /translation="MNKRHFSEKFYEKYGNRNDRPVLFFSPGRVNLIGEHTDYNGGFV
                     LPCALNYGTYLLLRKTGSKLLRLSTLNFNEDEELAVDGLFIKKAKSWINYPLGVMNEF
                     IKKGIDISGLDLLYYGDVPNAAGLSSSASIEMVTAVALNELFSAGLSTLEMVKMCQKA
                     ENEFVGMNCGIMDQFAVGFGKKDHAIFLNCDTLDYENVPVILEECSLIITNTNKKRGL
                     TDSKYNERRSECEKAVELLQSYKAVRNLSELNVTDLPHLPEYIHDPVVLKRAKHVISE
                     NDRVLEAVKVLKGNNLARFGELMNLSHDSL"
     gene            complement(923..2353)
                     /gene="dnaA"
                     /locus_tag="PLZ75_11410"
     CDS             complement(923..2353)
                     /gene="dnaA"
                     /locus_tag="PLZ75_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOS73041.1"
                     /translation="MNKTHHDVWNNCLQIIRDIIPSISYKTWFEPIVPLKLENNILTI
                     QVPSPFFYEYLEEQYIDILRKTLRREIGIDAKLEYNVVMENASYSDGKPYTVKYPSRN
                     KTDLHNKPLQVPFDVTQTSIKNPFVIPGIKKLHFDPKLNPDYNFNNFVEGECNRLARS
                     AGIAVGNNPGGTAFNPLMIYGDSGLGKTHLAQAIGILVKEKHPDKTVLYVNANKFQTQ
                     FVESVRNNNKNDFLHFYQMIDVLILDDVHEFGGKEKTQDTFFHIFNHLHQSGKQLILT
                     CDKPPVDLQGMEQRLLSRFKWGLQADLQKPDYETRLAILKKKAYNDGIELPDEIFEYL
                     AANISNNIRELESVLISLYAQSTLNRKEITLDLARMMVEKLVSSARKEITIDYIQKIV
                     CDYYKIPVDLIQGKTRKREIVQARQISMYFSKNLTKASLASIGSHIGGKDHATVLHAC
                     KTVNNLIDTDKHFRNQINEIEKKLKV"
     gene            complement(2690..3640)
                     /locus_tag="PLZ75_11415"
     CDS             complement(2690..3640)
                     /locus_tag="PLZ75_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOS73042.1"
                     /translation="MKISHEVKVGAVTLLTIAVFIWLFNFLKGKDFFRKTSYYYSVFD
                     DIGGLSESSPVEINGFKVGIVQAVRLLDRTSGKLLVEYSVSNDFRLPVNSVAEIVPVS
                     IIAGMKVQFRIGDGPGFYKVGDTIPGKLSKSIIVSIEEEFGPVKDKLLSLVAALDSVS
                     AAVSKMLDPEFRNNIDGIVANLSSTTGSINNIVKTREADLKNTIANLSAFSQLLSDNS
                     SNISSTLHNLRSISDTVEASGLNESLASLSTSLAETSKILENLNEGKGSAGQFLTNDS
                     LYANLSASLESLNLLLQDMKAHPKRYVHFSLFGKKNIPSE"
     gene            complement(3655..>4583)
                     /locus_tag="PLZ75_11420"
     CDS             complement(3655..>4583)
                     /locus_tag="PLZ75_11420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOS73043.1"
                     /translation="LKKNLKNVQVVYTRDDDSTVDLLDRPQIANRNKADLFISIHANW
                     ARNKNVSGTESYIMGLAKDEDNLAVAMKENEVILLEDDYSTRYQGFDPKSPESYIIFT
                     LMQNVFQKQSTDLAMKVQNQFRERANRNDRGVKQAGFWVLFNTTMPAVLIETGFITNA
                     AEEKYLNSNEGQEHLASAIFRACRDYINSIDNMSNFSMAEKETKETQPPAPADSAAAT
                     PGDGIVFMVQVASASSRSEIKPDQFRGINDIVEIHSQERYKYATGSFAEYADAVKYRR
                     QIEQIYPDAFVIALKDNKIIPLQQALDLKRKK"
BASE COUNT         1181 a          982 c          960 g         1460 t
ORIGIN      
        1 tgagtgagtc gtgggagagg ttcatcagtt ccccgaagcg ggcaaggtta tttcccttaa
       61 gcacttttac ggcttccaga acgcggtcgt tttcactgat tacgtgtttt gcccttttca
      121 ggacaacagg atcatgaata tattcaggca ggtgaggcag gtcagttaca ttaagctcgc
      181 tcagatttct aacagccttg tatgactgaa gaagttcaac cgccttttca cactccgacc
      241 ttctttcatt gtatttcgaa tcggtcagtc cacgtttctt gttggtattt gtgattataa
      301 gggaacattc ttccagaata accggtacgt tttcgtaatc gagtgtgtcg cagttgagga
      361 atatggcatg atctttcttc ccgaatccaa cggcaaactg atccatgatg ccgcagttca
      421 ttcccacgaa ttcattctct gctttctggc acatctttac catttcaaga gtgcttaatc
      481 cggcactgaa aagttcatta agcgcaacgg ctgtcaccat ttcgatggat gccgaggagg
      541 aaaggcctgc ggcattgggc acatcaccat agtagaggag atccagaccg gatatatcta
      601 tacccttttt gataaactca ttcatcacac cgagaggata gttgatccag cttttggctt
      661 ttttgatgaa aaggccatca acagccagtt cctcatcttc attgaaatta agtgtgctga
      721 gtcttaataa tttactgccg gtcttcctga gaaggaggta ggttccgtag ttaagggcac
      781 agggtaaaac aaatcccccg ttataatcgg tatgttctcc gataagattg actctgccgg
      841 gagagaaaaa aagaacaggc ctgtcgttcc tgttcccgta tttctcatag aatttttctg
      901 agaaatgcct tttgttcatt ttttatactt taagtttctt ttctatttca tttatctgat
      961 tacggaaatg cttgtcggta tcaatgaggt tgtttactgt tttacaggca tgaaggacgg
     1021 ttgcatgatc cttgcctcct atatgcgatc ctatgctggc cagggaggct ttcgtaagat
     1081 tcttggaaaa atacatggaa atctgacgtg cctgtacaat ttcccttttc ctggtttttc
     1141 cctggataag gtcaaccgga atcttgtaat aatcacagac tatcttctgt atataatcga
     1201 tggtgatttc ttttctggcg gagcttacca gtttttcgac catcatgcgt gcaaggtcaa
     1261 gggtgatttc cttgcggttg agggttgact gcgcataaag agaaatcagc actgactcaa
     1321 gttccctgat attgtttgat atgttggctg caaggtattc aaagatctca tccggaagtt
     1381 caattccgtc attatatgct tttttcttca gtattgccag tctggtttca tagtcgggtt
     1441 tctgaagatc ggcctgcagg ccccatttaa accttgacag gagtctttgt tccattccct
     1501 ggaggtcaac cggcggcttg tcgcaggtaa ggatcagctg cttgcccgac tgatggagat
     1561 gattgaatat atggaaaaaa gtatcctggg ttttttcctt tccgccaaac tcgtgaacat
     1621 cgtcaaggat gagcacatca atcatctgat aaaaatgaag gaaatcattt ttgttgttgt
     1681 ttcttaccga ttcaacaaac tgggtctgga atttattggc atttacatac agaactgttt
     1741 tgtcgggatg cttctccttt acaagtattc ctattgcctg ggcgagatgg gttttgccca
     1801 gaccggaatc cccgtagatc atgagaggat tgaatgccgt tccgccggga ttgttcccga
     1861 cggcaatacc agccgagcgt gcaagcctgt tgcattcccc ttccacaaaa ttgttgaaat
     1921 tgtaatccgg attaagtttc ggatcaaaat gtaatttctt gataccgggt atcacaaaag
     1981 gattctttat cgaagtctgg gtgacatcaa atggaacctg cagcggtttg ttgtgaagat
     2041 ccgtcttgtt gcgcgaagga tattttactg tataaggctt cccgtcagaa taactggcgt
     2101 tctccattac aacattatat tccagctttg catcaattcc gatctcccgc ctgagggtct
     2161 tgcggaggat atcaatgtac tgttcttcca ggtactcgta aaaaaacgga ctgggaacct
     2221 gaatggtaag aatgttattc tccagtttaa gaggcactat tggctcaaac catgttttat
     2281 aactgatcga aggtattata tcccttataa tttgcagaca gttattccag acatcgtgat
     2341 gcgttttgtt catttagaca ggttgtttta taaggacgta ttcaagctca tttcgtaagc
     2401 aatatttgta aaaaaaaaac ttaaaaaaaa aattattttt acttgatttt gtcaaaaaga
     2461 gtatatggtt ttatacctca ggaacttaat tttgtaaaaa tatttttctt tcataagtta
     2521 tagttaatca gtcatttaga catagcaatc ccctcttggt aatgcatacc aaccccttac
     2581 aaatacaata taacaatcaa acgactgtta agcaaaaatg cggaatatta tcacatgact
     2641 tcacatctat ttcacggcga taagatagtg cgaatttttt aatttccgct cattctgacg
     2701 gaatattttt tttcccgaac agagaaaaat gcacatatct tttcggatgg gctttcatat
     2761 cctgtaaaag cagattcaga ctttccaggc tcgctgacag gttagcatac agtgaatcgt
     2821 ttgtaaggaa ctgcccggca cttcctttgc cctcattaag attttcaagt atcttcgatg
     2881 tctcagccag gctggtgctg agtgatgcca gcgattcatt gaggccggaa gcttcaacgg
     2941 tatcgcttat cgatctgaga ttgtgtaagg tactgctgat attggatgaa ttatctgaaa
     3001 gtagctgtga gaatgcggaa agatttgcaa tggtattctt aagatccgct tcccttgttt
     3061 ttactatatt gtttatactg ccggtggtgc tgctgagatt tgccacaatc ccgtcaatat
     3121 tgtttctgaa ttccggatcc agcattttac tgactgcggc agaaacagag tccagggcag
     3181 ccacaagtga caacagttta tcctttacag gtccgaattc ctcttctatg ctcactatta
     3241 tggattttga aagtttcccc gggatggtat caccaacctt gtaaaaaccc ggaccgtccc
     3301 cgatacggaa ctgaaccttc attccggcaa tgattgaaac cggtactatt tcggcaacag
     3361 aatttacagg aagcctgaaa tcgttgctca ctgaatattc aacgagaagt tttccacttg
     3421 tacggtcaag gagtctgact gcctgaacga tccccacctt gaagccgtta atctctacag
     3481 gacttgattc ggatagtcct ccgatatcat caaaaacact atagtagtaa gatgtttttc
     3541 tgaaaaaatc ctttccttta aggaagttga aaagccagat aaaaacagct atagtcagca
     3601 gtgttactgc tcctactttt acttcgtgtg atattttcat tactgatttt tgttttattt
     3661 cttccttttc agatccagtg cctgttgtaa aggtattatt ttattatctt tcaaagcaat
     3721 gacaaatgca tcgggatata tctgctcaat ctgcctcctg tactttactg catctgcata
     3781 ttccgcgaag ctgcccgttg catatttata gcgctcctgg gaatggattt caacaatatc
     3841 gttaattccc ctgaactggt caggtttgat ttcagaccgg gaggatgctg atgcaacctg
     3901 aaccatgaaa actattccgt cacccggggt ggcagctgca gaatcagccg gtgcaggcgg
     3961 ctgtgtttcc tttgtttcct tttcagccat gctgaaattg ctcatattgt caatgctgtt
     4021 aatataatcc ctgcacgccc tgaaaatcgc ggaagccaga tgctcctgtc cttcattgga
     4081 attcaggtat ttctcctcgg cagcattggt gataaaccct gtttcaataa gcaccgcagg
     4141 catggtggtg ttgaaaagaa cccagaaacc ggcctgtttg acccctctgt catttctgtt
     4201 cgccctttcc ctgaactggt tctggacttt catggcaaga tccgtgctct gtttctggaa
     4261 gacattctgc atcagggtaa agattatata ggattctggt gacttcggat caaatccctg
     4321 atatctggtt gaatagtcat cttccagcag aataacctcg ttttccttca tggccacagc
     4381 cagattgtct tcatcctttg ccagtcccat gatgtaggat tccgttcctg aaacgttttt
     4441 attcctggcc cagtttgcgt gtatggatat aaaaagatca gccttgttcc ggtttgcaat
     4501 ctggggcctg tcaaggagat caacggttga atcatcatcc cgcgtatata caacctgtac
     4561 atttttaagg ttctttttga gat
//
LOCUS       DAOSPX010000271         4562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_17199_length_4562_cov_3.690260, whole genome shotgun sequence.
ACCESSION   DAOSPX010000271 DAOSPX010000000
VERSION     DAOSPX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17199_length_4562_cov_3.690260"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    305..404
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(501..1640)
                     /gene="nspC"
                     /locus_tag="PLZ75_11425"
     CDS             complement(501..1640)
                     /gene="nspC"
                     /locus_tag="PLZ75_11425"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOS73044.1"
                     /translation="MLINYDILPSPCFVMDEERFRKNLSLIRHVAGESGAEIILAFKG
                     FAMWGVFGIAREYISGAAASSLDEARLCFEEMGSPAHTYSVVYKEEEFDSIMELSSHI
                     TFNSLQQYRKYYDRLKKHTPVISPGLRINPEFSEITYSLYNPGAPGSRLGIRADDLGN
                     DLPEGIEGFHFHVLFESDSFALEKLLDAVENKFGRFFPRLKWINMGGGHLITGKGYDT
                     DHLIKLIRDFRKRTGLHVILEPGSAFAWETGELVATVEDIVVNRGIVTAILDVSFTAH
                     MPDCLEMPYKPKILGATDPVQGKPVYRMGGNSCLSGDFMGDWSFDRELKPGDRIVFLD
                     MIHYTMVKTTTFNGVHHPSIGVWTKEGKFRLLKKFGYEDYKNRLS"
     gene            complement(1871..3064)
                     /locus_tag="PLZ75_11430"
     CDS             complement(1871..3064)
                     /locus_tag="PLZ75_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOS73045.1"
                     /translation="MGKVLVIGAGGVGTVVVHKMASLPDVFTEILLASRTRSKCDAIA
                     ERVGTDRVKTAAIDADNVPELVRLIKSFRPDIVVNVALPYQDLHIMDACLETGVAYLD
                     TANYEPLDEAKFEYSWQWAYDDRYRDAGITAILGCGFDPGVTSVFTAYAAKHHFDELH
                     YLDIVDCNAGDHGKPFATNFNPEINIREVTQKGKYWENGKWVYTKPHEIHKPLTYPGI
                     GPRESYLIYHEELESLVKNFPTLKRARFWMTFGQEYLTHLRVIQNIGMAGIEPIMFDG
                     REIVPIRFLKAVLPDPGDLGENYKGWTSIGCRIRGIKDGKEKTYYIYNNCSHELAFRE
                     TGTQAVSYTTGVPATIGAMMYLKGIWKKPGVFNVEEFDPDPFMEQLNKLGLPWVELHD
                     VDLEL"
     gene            3210..>4562
                     /locus_tag="PLZ75_11435"
     CDS             3210..>4562
                     /locus_tag="PLZ75_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOS73046.1"
                     /translation="MKHNTITDYLCPQFLGFDMRFIHPPAIIRILSTNLLIEVFVFLI
                     CVPVALVYHESTLPFIWPAIITLSFSAAFFLLSRKSSVRKINIREGFLSVAMSWVIFS
                     LFGTLPYIFSRTIPRFTDALFESTSGITTTGSTVIANVEALPYSVNFYRALTHWAGGL
                     GIVLLVIIILPELGISAHQLLRRESSIREKIVPKTKSVGFRLLFIYLALTALQIILLS
                     AGDMNLFESICHSFSTIATGGFSIKNTSMMGYSPYSQYIMAIFMLFGGISFVIYYHFI
                     KLQFKKIGSNDEFWFYLAVILLSGTVATLILYGTTSDPFELSFRQGFFQTISIITTSG
                     FTTTDYLNWPHAGTFLIFILLFSGASTGSTTGGIKMARHLILIKSIRNVFIRLSHPSA
                     VTQIKLHGNTLSEKSITSVFSFILIYIFLFISGTLLITLTGTDPVTSASAVAASLGNT
                     G"
BASE COUNT         1214 a         1072 c          889 g         1287 t
ORIGIN      
        1 tggtaactcc ctgtatttat taacaatttg agtttaaaga cagacactaa ttaaaacatt
       61 tcaaagttga atgttaaact attaaataac attttatggt aatagtttca tttttgccaa
      121 tcacgaaatt ctgaaccgaa gtgaaaattg tttataaaac atcagaagct cacatactta
      181 aaatggatta gttacaatag gaattttact acagaagaat atgttagtgt tgcattgcca
      241 taacggattc ttatgttaca gggccttaat gtatccggat gagggagttt cagaagttac
      301 aggannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcttca ggagcttcag
      421 gagcttcagg agcttcagga gcttcaggag cttcaggagc ttcaggagtt tcaggatccc
      481 cggaaagggt ataataaggc tcaggacaga cggttcttat aatcctcgta accgaatttc
      541 ttcagcagac ggaatttccc ttcctttgtc cagacaccga ttgagggatg atgaactccg
      601 ttgaaggtag tggtcttgac catggtataa tgtatcatat caagaaaaac tatcctgtcg
      661 ccaggcttca gctccctgtc aaatgaccag tctcccataa aatcaccgga aaggcaggag
      721 tttccgccca tcctgtatac gggtttcccc tgaaccggat cagtggcacc aagtatttta
      781 ggcttgtatg gcatttccag gcagtcaggc atatgtgccg taaaggaaac atccagaatg
      841 gcagtgacta taccccggtt taccactata tcctccactg ttgccaccag ctcaccggtt
      901 tcccaggcaa aggcgcttcc cggttcaagg atgacgtgca atcccgttct tttcctgaaa
      961 tcccttatca gcttaataag atgatccgta tcatagcctt ttcccgtgat cagatggcca
     1021 cctcccatat taatccattt cagccggggg aaaaacctcc cgaatttatt ttcaaccgca
     1081 tcaagcagtt tttcaagcgc aaaggaatct gactcaaaca acacatgaaa gtgaaatcct
     1141 tcaatgcctt caggcagatc attgcccaga tcatccgcac ggatcccgag cctggacccg
     1201 ggagcaccgg ggttgtaaag gctgtaagtg atttcagaaa attcgggatt tattctcaga
     1261 cccggcgata tgaccggagt atgtttcttc agcctgtcgt aatacttcct gtactgctgt
     1321 aaagaattaa aggtgatatg tgaactcagc tccattattg agtcaaattc ctcttccttg
     1381 taaacaactg aataagtatg cgccggggat cccatctctt caaagcaaag acgtgcctcg
     1441 tccagcgagc tggcagccgc tcctgaaata tattccctgg caataccgaa cacgccccac
     1501 attgcaaatc ccttgaaggc aagaatgatt tcagctcctg attcaccggc tacatgcctg
     1561 ataagggaga ggtttttccg gaagcgctcc tcatccatta cgaaacatgg cgacggcaat
     1621 atatcataat tgattagcat ataattaatt tgttatggcc tgacatccgc tacaaggcaa
     1681 taccaggata ttataagtaa agtcttatgg ccttacatcc gccacacggc aactcctgga
     1741 tctttcatta aagtgatatg gccttacatc cgccacacga caactcatgg gtcttttagc
     1801 aaagtgttgt gtccttgcat ctgcaatgcg gcagtacgag gatctttcaa caaaagcaaa
     1861 acgccgggta ctacagctcc agatcaacgt catgaagctc cacccatggc agtccgagtt
     1921 tatttagttg ttccatgaag ggatccggat cgaattcctc aacattgaaa accccgggtt
     1981 tcttccagat acctttcagg tacatcattg ctccgatagt tgcaggaact cccgtagtat
     2041 aacttacagc ctgggttccc gtttccctga aggcaagctc atgactgcaa ttgttgtata
     2101 tgtagtatgt tttttccttt ccgtccttta tgccccttat cctgcagcct atcgacgtcc
     2161 atcccttata attctctccc aggtcaccgg gatcgggcaa tacggctttc agaaacctga
     2221 tgggaacaat ttcccttcca tcaaacataa taggctcaat acctgccatt ccgatattct
     2281 gaatcaccct aagatgggtc aggtattcct gtccgaatgt catccagaac cttgcacgct
     2341 tcagggtggg gaagttcttt acgagcgatt ccagctcctc atgatatatc agatatgact
     2401 ccctcggtcc tattcccgga taagtgagag gtttatggat ttcatggggc tttgtataaa
     2461 cccacttgcc gttctcccag tatttccctt tctgagtgac ctccctgatg tttatctcag
     2521 gattgaaatt cgttgcaaac ggctttccgt gatcacctgc attacagtca acaatatcaa
     2581 gataatgcag ttcatcgaaa tgatgttttg ctgcataggc ggtaaatacc gatgtaacgc
     2641 ccggatcaaa gccgcagcca agtattgcgg taatcccggc atccctgtac ctgtcatcat
     2701 atgcccactg ccagctgtat tcgaattttg cttcatccag aggctcgtaa tttgcggtat
     2761 ccaggtaggc cacacccgtt tccaggcagg catccatgat gtgaagatcc tggtacggca
     2821 atgccacgtt aaccactata tccggcctga acgatttgat cagccttacc agttccggaa
     2881 cattatcggc atcaatagcc gcagttttaa cacggtcagt gccaactctt tccgcaatgg
     2941 catcacattt ggacctggtc cggcttgcaa gaagtatttc agtaaaaaca tcaggaagag
     3001 atgccatctt atggaccaca accgtgccca ctcctcctgc accaataacc aatactttcc
     3061 ccatatctga attgaatttt taaggtttaa tcattcgggt tcatgttttc ctgcgatcaa
     3121 tactaacagg atttcaaata taggattaat aattgaccaa tcagcagggg tttgtcaggg
     3181 ttgatcaaca tactgatttt tgccggaaat tgaagcataa taccataact gattatcttt
     3241 gtccgcagtt tctggggttt gacatgagat tcatacatcc tccggcgata ataaggatac
     3301 tgagtacaaa tcttctcata gaggtttttg tatttctgat ttgtgttccc gtggcactgg
     3361 tttatcatga atcaactttg ccctttatct ggccggcaat tattacatta tcattttcag
     3421 cggctttttt ccttttatca aggaaaagtt ccgtgagaaa aataaatatc agagagggat
     3481 tcctgtctgt cgccatgtca tgggtcatat tttcactctt cggaaccctt ccgtatattt
     3541 tcagccggac aataccacgt ttcactgatg ccttattcga atccacttca ggaattacaa
     3601 ccacaggttc cacagtaata gcaaatgtcg aggcactgcc ctattcggta aatttttacc
     3661 gggcactcac acactgggcc ggaggactgg gcatagttct tcttgttatt ataattctcc
     3721 cggagctggg aatttcagca catcagcttt taagaagaga atcatccata agggaaaaaa
     3781 tagttcccaa aaccaagtca gtgggtttca ggcttctttt catatatctt gcacttacag
     3841 cccttcagat aattttgctt tcagcaggcg atatgaatct ttttgaaagt atttgtcact
     3901 ctttcagcac aatagcaacc ggtggctttt ccataaaaaa tacgagtatg atgggctatt
     3961 ccccttacag tcaatatatc atggctatct tcatgctctt cggcggtata agctttgtaa
     4021 tatattatca tttcataaaa ctgcagttca aaaagatagg cagcaatgat gaattctggt
     4081 tttacctggc agtaatactt ctgtcaggaa cagtggctac tctcatcctg tacgggacca
     4141 cgtccgatcc ctttgaactc tcattcagac agggtttttt tcagaccata tcaatcataa
     4201 ccacaagcgg ttttacaaca accgattacc ttaactggcc gcatgcaggc acttttctga
     4261 tttttatact cctgttttca ggtgcttcaa ccggttccac aaccggggga ataaagatgg
     4321 ccagacacct gatcctgatc aaaagcatcc gaaatgtatt catcaggctc agtcacccga
     4381 gtgcggtgac acagataaag cttcacggca atacattaag tgaaaaaagt ataacatctg
     4441 tattttcatt tattctcatc tacattttcc ttttcatatc gggaacactg ctgatcactc
     4501 tcaccggaac cgatccggtt acttcagcaa gtgcagtagc cgcttcactg ggaaataccg
     4561 gt
//
LOCUS       DAOSPX010000272         4557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_17220_length_4557_cov_4.224789, whole genome shotgun sequence.
ACCESSION   DAOSPX010000272 DAOSPX010000000
VERSION     DAOSPX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17220_length_4557_cov_4.224789"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLZ75_11440"
     CDS             <1..945
                     /locus_tag="PLZ75_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOS73047.1"
                     /translation="GDAVGSQLALFHYLKSRSIKAEMIAPNNLQEFLKWMEGSEKIRI
                     FISQRDKCRNIIEKADLIVMVDFNQPDRLGEMKDIVVRSGAVKVIIDHHLNPRGFADI
                     EISDPAFSSTTELVHEIVTQINGKPYFTRPYSEAVYVGIVTDTGNFEHGSYTSNTLRV
                     VADLLENGIEKNRILDLLFNNFSETRLRLQGFALNERMAILHEYNTAYIWLTRDDLNS
                     FRYVKGDTEGFVNLPLSIKGIIFSVFFVEKDGFVKLSLRSKGNFPVNEFASRFFSGGG
                     HLNASGGEFRGTLENSIRYFRKVLEENFEEFNGNNSLR"
     gene            942..1463
                     /locus_tag="PLZ75_11445"
     CDS             942..1463
                     /locus_tag="PLZ75_11445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOS73048.1"
                     /translation="MIFRVLIPAILLTGMFSCGQAPEKTGSGNLPGKKEMAELNRYLI
                     QKDRERIESYIERRDLKMNESPSGLWYQLKAEGEGDFLSDNDRIRMEYNCSLIDGRLC
                     YSSDEDGIEEIVLGRTDIPAGLAQGLKMLKNGGEAILIMPPHLAFGLKGDGKRIPARS
                     VIVYEIKVERMNK"
     gene            1577..2065
                     /locus_tag="PLZ75_11450"
     CDS             1577..2065
                     /locus_tag="PLZ75_11450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOS73049.1"
                     /translation="MRKILVFIMLAGLMVLNSCSLASKHEQYEREEKQKIQDYLNDNP
                     NLTFTLKESGLYYMDVSVGDGGSPAKGDTVYVHYTGYFLDGYKFDSNAGKPAYVFPAG
                     MGWVIQGFDEGIMLMRKGGKAKLLLPSGLAYGNSGYLMPAYTPLLFDVSLDSIVPGVG
                     KR"
     gene            complement(2084..2782)
                     /locus_tag="PLZ75_11455"
     CDS             complement(2084..2782)
                     /locus_tag="PLZ75_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOS73050.1"
                     /translation="MKGKLYLIPVTLGGNDVRAVIPDKVIETTKGLRYFVVEDIRSAR
                     RYLKLVCKGFPLDETRFLELNEHTPDEDMANYLDPALNGSDMGLLSEAGMPAIADPGS
                     KLIALAHRKTISVVPLSGPSSILLALVSSGLNGQEFVFHGYLPVKPADRAAKLKELER
                     NAGRGYSQIFMEAPYRNNRMLDTILSVCRNDTLLCIAADLTLPTEFIRTMKVSEWKRS
                     RPDLNRRPAVYIMQ"
     gene            2911..3678
                     /locus_tag="PLZ75_11460"
     CDS             2911..3678
                     /locus_tag="PLZ75_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS73051.1"
                     /translation="MKITFLGTGTSQGVPVIACDCNTCLSDDPHDKRLRTSLLLEKNG
                     LNLLFDAGPDFRQQMLRENVRKLDAIILTHEHKDHIAGMDDVRAFNYKSQDAIDIYGE
                     ERVLEAVKSEFSYVFAEYRYPGIPRMRLNAISDHSFRIKEVEILPVRVRHHHLEIYGF
                     RTGDFAYITDANYIPQESKEKLIGVKYLVINALRKEKHISHFTLREAIDFIREISPRK
                     AFLTHISHQMGLHSEVSRELPPGIFLAYDGLKFVCDE"
     gene            complement(3681..3842)
                     /locus_tag="PLZ75_11465"
     CDS             complement(3681..3842)
                     /locus_tag="PLZ75_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73052.1"
                     /translation="MTGCASSKQNPYHKKRTEPVKKVNPQQLGKNRYYYSPNYQKKLI
                     RGYKKKKKY"
     gene            4131..>4557
                     /locus_tag="PLZ75_11470"
     CDS             4131..>4557
                     /locus_tag="PLZ75_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73053.1"
                     /translation="MTLSTGNHNIILFLLLFFPFSLYSQEIRQIGVEATRGLITGRVY
                     LSDSTVLNPDIPRPLYSFHCNGKYYSSADQPAVKTDSVFSQILRNKLKITFKIPGFSD
                     PDWICDLSLSNTGNDTLRISNVVPYGEDRSEVYITGFGPP"
BASE COUNT         1338 a          890 c         1068 g         1261 t
ORIGIN      
        1 ggtgatgctg ttggttcaca actggcgctg tttcattatc ttaagtcacg cagcataaag
       61 gctgaaatga ttgctccgaa taaccttcag gaatttctca aatggatgga aggctcggag
      121 aagataagga tttttatttc acaaagggat aaatgcagga acatcattga aaaagctgac
      181 ctgatcgtta tggttgattt caatcagcct gacaggctcg gggagatgaa agatattgtg
      241 gtccgctcag gggctgtgaa ggttattatt gatcatcatc ttaatccgag gggatttgcc
      301 gatattgaaa tatctgaccc tgcgttttcc tcaactaccg agcttgttca tgagatagtc
      361 actcagataa acgggaagcc ctatttcacc aggccctatt cggaagcagt gtatgtggga
      421 atagtaaccg ataccggcaa tttcgaacat ggcagctata caagcaatac cctgagggtt
      481 gtggctgacc tgctggagaa cgggattgaa aaaaacagga tactggacct gctgttcaat
      541 aatttttcag aaacacggct gaggctccag ggatttgccc ttaatgaaag gatggcgatc
      601 ctccatgaat acaacactgc atatatctgg cttaccaggg atgatctgaa ttcattcaga
      661 tatgtgaaag gcgacacgga gggttttgtg aacctgccat tgtcaattaa gggaataatc
      721 ttttcagtct tttttgttga aaaagacggg ttcgtgaaat tatcactgag atcaaaggga
      781 aatttccccg taaatgaatt tgcctcccgc ttcttttcag gcggaggaca tcttaatgct
      841 tcgggcggcg aattcagggg aacactcgaa aattcaatca gatacttcag gaaagtactg
      901 gaggaaaatt tcgaagagtt taacggcaac aatagtctca gatgatattc agagtgttga
      961 tacctgccat attgctcaca ggcatgtttt cctgcgggca ggctccggaa aaaaccggca
     1021 gtgggaacct tccgggcaaa aaggaaatgg ccgagctcaa cagatatctt attcagaaag
     1081 acagggaaag aattgaaagt tatattgaaa gaagggatct gaaaatgaat gaaagtcctt
     1141 ccggcctgtg gtatcagctg aaggctgagg gtgaaggtga tttcctgtct gataatgaca
     1201 ggatcagaat ggagtataat tgttcactta ttgacgggag gctttgctat tcatcagatg
     1261 aagacggcat agaggagatt gtgcttggca ggaccgatat accggcagga ctggcccagg
     1321 gtttgaagat gcttaagaat ggtggtgagg ccattctgat catgcctcct cacctggctt
     1381 tcggactgaa gggggatggt aaacgaattc ctgccagatc tgttattgta tatgagataa
     1441 aggttgaaag aatgaataaa tgaccggaga gaatccggat caggttgatg agaattaact
     1501 aaatacgtgg aaaatattga gaaaacagta aaaatctgag ataataataa ccatgattaa
     1561 cataaattta ttgaaaatga gaaaaatact ggtgtttatc atgttagcgg gcctgatggt
     1621 tcttaattcc tgcagcctgg cttcgaaaca tgaacaatat gagcgggaag aaaagcagaa
     1681 aatacaggat taccttaatg acaatcccaa tctgacattc acattaaagg aaagcggatt
     1741 gtattatatg gacgtatccg tgggagatgg cgggagtccc gcgaaaggcg atacggttta
     1801 tgtacattat accggctatt tcctggatgg ttacaagttt gattcaaatg caggcaagcc
     1861 ggcttacgtt tttcccgcag gaatgggctg ggtaatacag ggttttgatg aaggtataat
     1921 gctgatgaga aaaggaggga aggcaaagct ccttttgcca tccggcctgg catacggcaa
     1981 ctcgggatat ttaatgccgg catatacacc cctgttgttt gatgtatcac tggacagcat
     2041 agttcccggc gtaggcaaaa ggtaggaaga ttgcatactg tgattattgc ataatataaa
     2101 cagcaggcct cctgttcaga tcgggtctgc ttcttttcca ttccgatacc ttcatcgttc
     2161 tgatgaattc cgtcggaagg gtcaggtcag ctgctatgca cagcagggtg tcattcctgc
     2221 agacactcag tattgtatca agcatcctgt tgtttctgta gggtgcttcc atgaagatct
     2281 gggaatatcc tctgccggca ttcctttcaa gttcctttaa ttttgccgcc ctgtcagcgg
     2341 gttttaccgg aagatatccg tgaaacacga attcctggcc attcagtcct gacgatacca
     2401 gggcaagaag aatagaggac ggtccggaga gaggtacaac actgatcgtt ttcctgtggg
     2461 caagtgcaat gagctttgaa cccggatctg caatggcggg catgccggcc tcactcagca
     2521 gacccatatc cgaaccgttc aaagccgggt cgagataatt cgccatgtct tcatcaggag
     2581 tatgttcatt aagttcaagg aaacgggttt catccagtgg aaagccctta cagaccagtt
     2641 taagatacct tctggcactg cggatatcct caacaacaaa gtatctgaga ccttttgtcg
     2701 tttcaatcac cttgtcggga ataactgccc tgacatcatt tcctccgagt gtgaccggaa
     2761 taagatatag tttgcctttc acaagtgata agtttatcaa atataacaaa atccgtggag
     2821 tcgcaaaacc ggattatgtg gtaaaaaaca ggttctgtca gtttgttgtt attgattttt
     2881 aatataggtt tgccggtaaa ttaatctgtt gtgaagatca ctttcctggg aacgggcaca
     2941 tcacagggag tacctgtgat tgcctgtgat tgtaacacat gtttgtctga tgatccgcat
     3001 gataagaggc tacggacttc cctgctcctt gaaaaaaacg gactgaacct gctgttcgat
     3061 gccgggcctg atttccggca gcagatgctc agggaaaacg tcaggaaact ggatgccata
     3121 atccttacac atgagcataa ggatcatatt gcgggaatgg atgatgttag ggcattcaat
     3181 tataaaagtc aggatgccat agatatttat ggggaagaac gggtactgga agcagtgaaa
     3241 agtgagtttt catacgtttt tgctgaatac cgctatcccg gaatacccag aatgaggctg
     3301 aatgccataa gtgatcatag tttcagaata aaggaagtgg aaatactgcc ggtaagggta
     3361 agacatcatc atctggaaat ctacggattc cgtaccggtg atttcgccta tatcaccgat
     3421 gccaactata tcccccagga aagcaaggaa aaactgatcg gggtaaaata tctggtcatt
     3481 aatgcattac gcaaggagaa gcatatttca catttcaccc tcagggaagc aattgatttt
     3541 ataagggaga tatctccaag aaaggctttt ttaacgcata taagtcacca gatgggactt
     3601 cacagtgaag taagcaggga attgcccccg ggtattttcc tggcttatga cggactgaaa
     3661 ttcgtatgcg atgaatgaat ctaatacttc tttttcttct tataacccct tatcagcttc
     3721 ttctgatagt tgggcgaata gtaatacctg tttttgccaa gttgctgggg gttgaccttt
     3781 tttacgggtt cagttctttt tttatgatag ggattctgct ttgatgaagc acatcctgtc
     3841 attaagacaa ttgaaccgaa taatactaaa tattttaata ttctgactat tccggacatc
     3901 cttttatctg aatcagaaaa gaccgtccaa tatacaacaa ttaattcaaa aaagcaaatt
     3961 aaaagcttat tattaagctt tttggctcag taaacaggga gaaagaaata tttttcatac
     4021 aggaaagcat ataatccgga tattttaatg tttaaatttt atatttgaaa attagctgtt
     4081 taacaaaacg gaaacccgaa atcaagatgc tgaaacatca ttctcaatat ttgacattat
     4141 caaccggaaa ccataatata attttattcc tccttctgtt ttttcctttc tccctatatt
     4201 cccaggagat acgacagata ggcgtcgagg caacgagagg cctgataacc ggaagggtct
     4261 atttatctga cagcactgtt cttaatcctg atattcccag gcctttatac agttttcact
     4321 gcaacgggaa atattatagt tccgcagatc agcctgctgt taagactgat tccgtattct
     4381 cgcagatact tagaaacaag ctgaaaatca cctttaaaat cccgggcttc tctgatccgg
     4441 actggatctg cgatctcagt ctcagcaata cgggaaatga caccctgaga atatccaatg
     4501 tggttcccta cggggaggac aggtctgaag tgtatatcac gggtttcggg cctcccg
//
LOCUS       DAOSPX010000273         4497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_17510_length_4497_cov_3.936965, whole genome shotgun sequence.
ACCESSION   DAOSPX010000273 DAOSPX010000000
VERSION     DAOSPX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17510_length_4497_cov_3.936965"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLZ75_11475"
     CDS             complement(<1..382)
                     /locus_tag="PLZ75_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOS73054.1"
                     /translation="MKTRQFFILLLTFLLSVSWISAQKADERGYIVKTGDMAPDFTIR
                     EAGGRTYKLSDLRGKIVMLQFTASWCGVCRKEMPFIEKEIWLPKKNSGLVVIGIDRDE
                     PEETVLRFKKDMGISYPLALDPGNR"
     gene            502..2100
                     /locus_tag="PLZ75_11480"
     CDS             502..2100
                     /locus_tag="PLZ75_11480"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOS73055.1"
                     /translation="MKGKPAILLVYMLNISILNAQLTAVEKGLHAITTDAIMAQLGFL
                     ASDWTEGRAAGERGEYLAGDYIASMLRLYGVKPGGDHSGRTDYTITGAAGERSYFQNF
                     SLIRALPSGEPSVSISIRSGDGTRTINLANNVDFSVRPLYRSVRTEAPVVFAGYGFRN
                     EKIKFDDFRKTDIRGKFILIISGYPEFADKRLTPHEINTSLRAAEDYAREKGAAGILE
                     FDPGLLLAGKAPEPEFLNMSPAERSRGPYTYRPQYYLPGKTMPDNLIRIRISAAAAAE
                     ILKGSGISISEYIKHAGQNRSYDLPMLSGRSVSFSSDAILSQVAVRNVIGIIEGDDTD
                     QAIVIGAHYDHLGMKDGYIWNGADDNASGTVGALMLAKALKATGVKPGKTIIIALWSS
                     EETGLLGSRYFVRNPVIPLENIRMNLNFDMISRYISEENKKGVDMTYTASRALFRDIT
                     EANLKKYGIDLEVNYHPSDNPPGGSDHRSFVEAGIPVMRFKPGHREEYHTPSDEIHTI
                     DQDIMEKIIRISFVNIWDLANSEW"
     gene            2176..3066
                     /locus_tag="PLZ75_11485"
     CDS             2176..3066
                     /locus_tag="PLZ75_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOS73056.1"
                     /translation="MKVLVTIFQIIALFLFSQTVPAQDSQAEKLHIIVIGAHPDDPDK
                     VGGTAYKWAQLGHDVLLVSLTSGDAGHQSIKPAKLAKIRREEARKAGETIGVKYITLG
                     NHDGKLMPTYKNRLEVIRLIREHKADIVIFPRPYDYHPDHRYTGTLVLDAAYMVTVPR
                     ILPGVPHLDKNPLFLYMSDGFVNPVPFRPDVCVIIDDVIEKKIDMYHQHTSQMYEWLP
                     YNRGGLAEVPATDSERREWLGRTRKNGSSAEPYREKLIEMYGPEKGGGARYCEAFQDS
                     GYGTRLTKNNLRHYFPFIDN"
     gene            complement(3053..3418)
                     /locus_tag="PLZ75_11490"
     CDS             complement(3053..3418)
                     /locus_tag="PLZ75_11490"
                     /inference="COORDINATES: protein motif:HMM:NF021415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HOS73057.1"
                     /translation="MEKKCLDCGESIRGRTDKKFCSDQCRNNYNNRLNRDTNNFVRNV
                     HGLLRKNRRILADLYREGMLRVHKDALFALGYNFSFFTHIIESTGGYKYYYCFEYGFR
                     EAGNDFIELKENSRYLNYQ"
     gene            3850..4239
                     /locus_tag="PLZ75_11495"
     CDS             3850..4239
                     /locus_tag="PLZ75_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73058.1"
                     /translation="MKKSGFVLLIMLAFSCATSNKSAMMHEDELFITRKYVGEFVDSR
                     HSDPVKIGSPHLIWIKTTQDTVYGKISTYARKCEFSEGDRLYLRRVYQSPGPFGYWMY
                     QIENDNNIWYRISEFQHDNKVLVQTWF"
BASE COUNT         1273 a         1008 c         1041 g         1175 t
ORIGIN      
        1 gccgattacc cggatcaagt gccagcggat agcttatacc catgtccttt ttaaacctga
       61 gcacggtttc ttccggttca tcccggtcaa tgcctataac aaccaggcct gaattctttt
      121 tcggaagcca tatttccttt tcaataaaag gcatttcctt acggcatact ccacaccagc
      181 ttgcagtgaa ctgaagcatg acaattttcc ccctgaggtc cgaaagtttg taggttctgc
      241 ccccggcctc cctgatggtg aaatcaggag ccatgtcacc cgtttttaca atatagcccc
      301 gttcatcagc tttctgagca ctgatccatg atactgacag gaggaacgtc agtaacagaa
      361 taaaaaattg tcttgttttc atttcctata ccgcataaat agtttaaata ccatttttct
      421 ttaacaaaaa tactgtacag cttgttcatt ctcttttccg ttctgattat attagtaatt
      481 tagtgaaaaa ttgagattca gatgaaggga aagcctgcca ttcttttggt ttacatgttg
      541 aatatcagca ttttgaatgc gcagcttacc gcggttgaaa aaggtctgca cgcaattaca
      601 acagatgcca tcatggccca gctcggattt cttgcctcag actggacgga aggacgggca
      661 gcaggagaac gcggtgagta cctggcaggt gattatattg caagcatgct gcggctgtac
      721 ggtgtaaaac ccgggggaga ccattccggg agaactgact ataccataac aggagcagcc
      781 ggagaaaggt catattttca gaatttttca ctaatcaggg ctcttccttc cggggagcct
      841 tcagtcagca tttcaatccg ctcgggagac ggcacaagga caataaacct tgccaataac
      901 gtggattttt ctgtacgccc cctttacagg tccgtccgga cagaagctcc cgtagtattt
      961 gccggttatg gtttcagaaa tgaaaaaatt aaattcgatg atttcaggaa aactgatatc
     1021 aggggcaaat tcatattaat aatatcagga tatcccgaat tcgctgataa aagacttacg
     1081 ccacatgaaa taaatacatc cctgcgtgct gctgaagatt atgccaggga aaaaggcgca
     1141 gccggaatac ttgaattcga tcccggcctg ttattggccg gaaaagcacc ggaacccgaa
     1201 ttcctgaata tgtcacccgc cgaaagaagc cggggaccgt atacatacag gccgcaatac
     1261 tatcttcccg gaaaaaccat gccggataat ctgataagaa taagaatctc ggcggcggca
     1321 gcggcggaaa tactaaaggg atcaggcata agcatttccg aatacatcaa gcatgccggg
     1381 cagaacagat catatgacct gccgatgctg agcggcagat cggtcagctt cagctctgat
     1441 gcaatattgt cgcaggttgc agttagaaac gtcattggaa ttattgaggg tgatgatacg
     1501 gatcaggcga ttgtgatcgg tgcccattat gatcacctgg gcatgaaaga cggttacatc
     1561 tggaacggcg ccgatgataa tgcttccgga accgttggcg cactcatgct ggcaaaggcc
     1621 ctgaaggcaa ccggtgtgaa acccggcaaa accataatta tcgcactgtg gtcatcagaa
     1681 gagacaggac ttctcggctc aaggtatttt gtaaggaacc cggtcattcc cctggagaat
     1741 atccggatga accttaattt tgatatgatc tcaagatata tttcggaaga aaacaaaaaa
     1801 ggggttgaca tgacctatac tgcttcccgg gcccttttca gggatattac ggaagccaat
     1861 ctgaaaaaat atggcatcga cctggaagtt aattaccatc cttccgacaa tcctcctgga
     1921 ggcagcgacc acaggtcatt cgtggaagcc gggataccgg taatgcggtt caagccaggc
     1981 cacagggagg agtatcatac tccttccgat gagatccaca caattgatca ggatataatg
     2041 gaaaagatca ttcgcatcag ttttgtgaat atctgggatc tggctaacag tgaatggtga
     2101 aaacagtcag gccggcatcg gtacaaatgg aacatgcgcc gggaaacagg gagttcaaca
     2161 tttaaatttt atgatatgaa agtgcttgta acaattttcc agatcattgc actgtttttg
     2221 ttttcgcaaa cagtaccggc acaggacagc caggcggaaa agcttcatat aattgtcata
     2281 ggtgcacacc ctgacgatcc cgacaaggtc gggggaactg cctataaatg ggctcagctt
     2341 ggtcatgatg tacttctggt ttcacttacc agcggtgatg ccggccatca gagtattaag
     2401 cctgcaaaac ttgcaaagat caggagagaa gaggcgagaa aagccgggga aacaataggt
     2461 gttaaataca ttaccctcgg taaccatgac gggaaactta tgcctacata caaaaacagg
     2521 cttgaagtga ttaggctgat aagggagcac aaggctgata ttgttatttt tccccgcccg
     2581 tacgactatc atcccgatca cagatatacc ggaactctag tcctggatgc ggcatacatg
     2641 gtcactgttc cgaggatact tcccggagta ccgcatctgg ataagaatcc cctcttcctg
     2701 tatatgagtg acgggtttgt caatcccgtg ccgttcaggc cggatgtatg cgttatcatt
     2761 gatgatgtca tcgaaaaaaa gattgacatg taccatcagc atacttcaca aatgtacgaa
     2821 tggctgccgt ataacagggg cggactggcc gaagttccgg caaccgactc tgaaagaagg
     2881 gaatggcttg gcagaacacg caagaatggc tcaagcgcag agccttacag ggaaaaactt
     2941 attgaaatgt acggaccgga gaaaggtggc ggtgccaggt attgtgaagc cttccaggat
     3001 tcaggatatg gaacacgtct tacgaaaaat aatctcaggc attactttcc cttcattgat
     3061 aattgagata gcggctgttc tccttaagtt cgatgaagtc gttgcctgct tcccggaaac
     3121 catattcaaa acagtaatag tacttatacc cgccggtgct ttcaataata tgggtaaaga
     3181 agctgaaatt gtaaccaagt gcaaacaatg catccttgtg tactctcagc attccctccc
     3241 tgtaaaggtc ggcaagtatc ctcctgttct tcctcagcag tccgtgcaca tttcggacaa
     3301 aattgtttgt gtcacggtta agcctgttgt tgtaattgtt cctgcactga tcggaacaga
     3361 actttttatc tgtgcggcca cggattgatt cgccgcagtc gagacatttt ttttccacag
     3421 cactgaagtt ttgataaaga taataaaaac cgtttataaa cggttaaaaa cgactactga
     3481 aacttttatc cgctgctcag aatttgactg acagcaggca gaaccctttc cggtgaaaca
     3541 gttttcagct ttaaagccct ttgcaatacg attttttgta aattttgacc aatgtctcca
     3601 aatttgtcat cagtcaacgg cacaagtaag cgaagcctgt gatacggctg acagaaaacc
     3661 ccgaaaaaca ggtttaaagc ggggcccttg gggttatctg tttgcaaacc atatcattaa
     3721 acattatctt tggcatgagg ctgcaaagga ttttccggca gtttcattat tgatttaatt
     3781 cttcatcgta cccgttgact catgagggtt ttctgtcagc gggaagtgtt ggttaaaagt
     3841 tatcaggtca tgaaaaaaag cggatttgtt ctgttaataa tgcttgcctt ttcctgcgcc
     3901 acctcaaaca agtcagcaat gatgcatgag gatgaattgt ttatcacacg caagtatgta
     3961 ggtgagtttg tggattcaag gcacagtgat ccggtaaaaa tcggatcacc gcaccttatc
     4021 tggataaaaa ccacacagga cactgtttac gggaagatat caacctatgc aaggaagtgt
     4081 gaattttcgg aaggcgaccg gctttatctg aggagggtgt accagtcacc gggtccgttt
     4141 ggatactgga tgtaccagat tgagaatgat aataatatct ggtacaggat cagtgagttc
     4201 cagcatgaca acaaggttct ggtccagacc tggttctgac aataaaattc cggatgccgc
     4261 ttactcagac tgaaaatggc acccggaacc ttatttatcc ggatctgagg aataactgca
     4321 gatcagttta tcttctgtgc aatttcattt accaggcgga aatcagaatc agtgtcgcgg
     4381 ggattccatg gtgttccctc tccggactca ccaagcgatt tcaatatgtc gccggcttca
     4441 tttttcctgc cttcctgcat cagccgggcc cattccttta ttttatcatt ccccgga
//
LOCUS       DAOSPX010000274         4440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_17778_length_4440_cov_4.333865, whole genome shotgun sequence.
ACCESSION   DAOSPX010000274 DAOSPX010000000
VERSION     DAOSPX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17778_length_4440_cov_4.333865"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLZ75_11500"
     CDS             <1..734
                     /locus_tag="PLZ75_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS73059.1"
                     /translation="SWAYLNGVIHDYNLNIIDDNPGSIATAGIIPRTEITNRFWYNEN
                     LVYSHYMLPGILAILVTAIGFLLAGLNLVKEKEAGTIEQINVTPIRKHQFITGKMVPF
                     IIIGLFDLALGLALGKLVFRIPFEGSIGLFFLCAAIFMISVLGIALFISNFSSSQQQF
                     MFVAYFFMMILILMSGIFTPFESMPSWAIKFNMANPMAYLIRINRMIMLKGSGFRDIY
                     RDIASLAVLAVIFTAFAVRRYRKTA"
     gene            complement(827..1690)
                     /locus_tag="PLZ75_11505"
     CDS             complement(827..1690)
                     /locus_tag="PLZ75_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOS73060.1"
                     /translation="MKKVLFGFVLMSSMVFLLCSCKSEKGTLSVMTFNIRYDNPHDSA
                     NAWPNRIPVVCDFLNAEKPDLIGMQEVLYQQYTVLDSALSDYGSVGVGRTDGLKAGEM
                     NPVFFRKDRLELIRTKTFWLSETPEVAGSMAWGAGLPRIVTWVELADKENDLHFYYFN
                     THFAHDSDSARTRSARLLLEKADSIAADFPIIITGDLNMLPESEGYSVLTGPFESVPL
                     LRDSYEISKKTPEGPVYTFNNFRDKPGPGRIDFILVRDGMKVLDHRTVIRKEKGVFIS
                     DHWPVIATVAF"
     gene            1846..3654
                     /gene="mutL"
                     /locus_tag="PLZ75_11510"
     CDS             1846..3654
                     /gene="mutL"
                     /locus_tag="PLZ75_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOS73061.1"
                     /translation="MPDIIKLLPDSVANQIAAGEVIQRPASVVKELMENAVDAGATAI
                     NVIIRDSGRTLIRVTDDGSGMSETDARLSFERHATSKISSANDLFSIKTKGFRGEALA
                     SIAAVAMVELKTRREDEEVGTMIAINGSRVESQEPCSCPKGSNFSVKNLFYNIPARRK
                     FLKSDNTEMRNIIIEFQKVALTHPDIKFTLLHNDTEVYNLSPANNRQRIIGIFGRQIN
                     RELIPLETETTIVNVKGFITKPEFVRRSCRDQFFFVNRRYMRHPYFHKAVMEAYQKII
                     PPDTIPSYFIFMETDPETIDINIHPTKTEIKFENERAVWQILMASVREALGRFNIAPS
                     IDFENEALVDIPVRTASSPVPEKPLIPVNPLFNPFENEEDASRKPDFISRFGRENVTN
                     WEKLYPAGFRSSGQSVIPGSPEDTERKFFQIKSKYIVCPVKSGLMLIDQKRAHERVLY
                     DRYMESLNRHSPASQTELFPVPIDIDPVDYSLLREIEPELKYLGFIIQFREKNRIIIK
                     GRPSAAVNTDPKDMLDILIAEYKNAPSGPASGAGEKVAAAMASASAIPYGKILFQKEM
                     EDLFDSLFACPSPNYSPGGKPVISIITMEELDRKFR"
     gene            3743..>4440
                     /locus_tag="PLZ75_11515"
     CDS             3743..>4440
                     /locus_tag="PLZ75_11515"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOS73062.1"
                     /translation="MNGFGRGFLTLPTVVKNLIMVNVIMLLATWVIGSALNYNLTGLL
                     GLYFPKSESFKPLQILTHMFMHAGFWHLFFNMYALFLFGQILENVWGPKRFLIYYLVC
                     GLGAALVHETVIGLEYNNLIKTLSPEQVHTVINEGASLLSRNRQFADPGMQKLQLLLN
                     TPTVGASGAVFGILLAFGVLFPNTQLMLLFPPIPIRAKYFVLAYGGIELYLAFTQPGS
                     NIAHAAHLGGMLFG"
BASE COUNT         1235 a         1028 c          988 g         1189 t
ORIGIN      
        1 tttcctgggc ttatctcaac ggtgtcattc acgattacaa cctgaacatt attgatgata
       61 atcccggaag cattgccaca gcaggaatta ttccccgcac tgaaattaca aacaggttct
      121 ggtacaatga aaacctggtt tacagccatt acatgcttcc gggaatactg gctatactcg
      181 tcactgccat cgggtttctt cttgcagggc ttaaccttgt aaaggaaaag gaggcaggca
      241 ccattgaaca gataaatgtg acacccataa gaaagcatca gttcattacg gggaagatgg
      301 ttcccttcat cataataggg cttttcgatc tggctctcgg tctggctctc gggaagctcg
      361 tcttcagaat acccttcgag ggaagcatag gattgttttt tctctgtgct gcaatcttta
      421 tgataagtgt actcgggata gcgctgttta tatccaattt ttcgtcaagt cagcaacaat
      481 tcatgttcgt tgcatatttc ttcatgatga tacttatact aatgagcggt atattcactc
      541 cctttgaaag tatgccatcc tgggcaatta agtttaacat ggccaatccg atggcttacc
      601 tgataaggat taaccgtatg atcatgctca agggatcagg ttttcgtgat atatacaggg
      661 atattgcctc cctggcggtt ctggcagtaa tattcaccgc ctttgcagtg aggagataca
      721 gaaaaaccgc atagaaacag ccggcagaat aaatcgtctg cgctgcctgg agcagcgggg
      781 caaactgtct gcgctgccgt ggcaaaggac atcgtcagcc ggttcattaa aatgccactg
      841 ttgcaataac gggccagtga tccgatatga aaacaccttt ttcctttctg atcactgttc
      901 tgtggtcaag aaccttcatt ccgtccctta ccaggataaa gtcaattctt cccggtccgg
      961 gtttgtccct gaaattattg aaagtatata ccggtccttc gggagtcttt ttcgatattt
     1021 catatgaatc cctcaacagg ggaacacttt caaaaggtcc ggtcagcact gaatatcctt
     1081 cactctccgg aagcatgttc agatcccccg ttatgatgat agggaaatcg gcagcaatgc
     1141 tgtcagcttt ttcaagaaga agcctggcgc ttcttgttct tgcggaatcc gaatcatgcg
     1201 caaaatgagt gttgaagtaa tagaaatgaa ggtcattctc cttatcggcc agctcaaccc
     1261 aggtcacaat ccttggcaat cccgcccccc atgccattga tccggcaact tcgggagttt
     1321 cggaaagcca gaatgttttt gttctgataa gctctaacct gtcttttctg aaaaagacgg
     1381 gattcatttc acctgccttt aagccgtcag tcctgccaac gcctaccgat ccgtaatcac
     1441 ttaaggctga atcaagcaca gtatactgct ggtaaagaac ttcctgcatg ccaataagat
     1501 cgggcttctc tgcattcagg aagtcacata cgacaggtat tctgttcggc caggcattgg
     1561 cgctgtcgtg cggattatca taccggatat tgaatgtcat tacactcagc gttccttttt
     1621 ctgacttgca ggaacaaagc aaaaaaacca tggatgacat cagaacaaat ccaaacagaa
     1681 cttttttcat ctctcctcta tttttttcaa agataagtat cctttaagaa ttccggtaat
     1741 cttcttctgc caatgaatga aaagctatcc ggcaattatt gatatatttg ccggtatcat
     1801 ggaaagttta aacctgcctg tcctattgtt tcaatttaag tactgatgcc ggatataata
     1861 aaattactgc ccgactctgt tgccaaccag attgctgccg gagaggttat ccagagacct
     1921 gcttcagtgg tcaaggaact catggagaat gccgttgatg ccggagccac tgccataaac
     1981 gtgataatca gggattcggg aagaacgctc atcagggtaa cggatgacgg aagcggcatg
     2041 tcggagactg acgcaagact atcgtttgaa aggcatgcca catcaaaaat atcatcggca
     2101 aatgatcttt tcagcattaa gacaaagggg ttccgcggag aagcccttgc atcaatagcc
     2161 gcagtggcca tggtggaact gaaaacccgg agggaggacg aggaggtcgg aacaatgata
     2221 gcgataaacg gatcaagggt tgaaagccag gaaccctgca gctgtccgaa aggctcaaac
     2281 ttctccgtga aaaatctttt ttacaacatt ccggcaagga ggaaattcct gaaatccgat
     2341 aataccgaaa tgaggaatat tattattgaa tttcagaaag tggcactgac gcatcccgat
     2401 ataaaattca cactcctgca caacgatacg gaagtatata acctttcacc ggcaaataac
     2461 cgtcagagga taataggtat atttggcagg cagataaacc gggaacttat tcccctggaa
     2521 acagaaacca cgattgttaa cgttaaaggc ttcataacca agccggaatt tgtgcgccgt
     2581 tcctgcaggg atcagttctt ctttgtgaac aggcgctaca tgagacatcc ttattttcat
     2641 aaggctgtta tggaagcata tcagaaaatc attcccccgg atacaattcc ttcctatttc
     2701 attttcatgg aaactgatcc ggaaacaatt gacataaata tccatcctac aaaaaccgag
     2761 atcaagttcg agaatgaacg tgccgtctgg cagattctca tggcatccgt cagggaggcc
     2821 ctcggcaggt tcaacatagc cccctcgatc gattttgaga atgaagcact tgtggacata
     2881 ccggtgagaa ctgcctcgtc acccgttccg gaaaaacccc ttattccggt aaatcctctt
     2941 ttcaatcctt ttgaaaatga agaagatgcc agccgaaaac ctgattttat ctcaaggttc
     3001 ggccgggaga atgtcacaaa ctgggaaaaa ctataccctg ccggcttccg gagcagcgga
     3061 cagtccgtaa taccgggaag cccggaagat actgaaagga agtttttcca gataaaaagc
     3121 aaatacattg tttgtccggt aaagtccgga ctgatgctga ttgaccagaa aagggcacat
     3181 gaacgtgtat tgtatgaccg gtatatggag agcctgaaca ggcattcccc ggcaagccag
     3241 actgagcttt tccctgttcc cattgacatt gatcctgtgg attacagtct gctcagggaa
     3301 attgagccgg aactgaaata tcttggtttt atcattcagt tcagggagaa aaacaggata
     3361 ataatcaagg gaaggccttc agctgcggtt aatactgatc ctaaggacat gctggatatt
     3421 ctgattgcag aatacaaaaa tgctccttcc ggtccggctt ccggagccgg tgaaaaagtg
     3481 gctgcggcaa tggcttcagc ttcagccatt ccttatggga aaatattgtt ccagaaggaa
     3541 atggaagatc tttttgacag cctttttgct tgtccctcac ccaattattc acccgggggc
     3601 aaacctgtga taagcattat taccatggag gaactcgaca gaaaattcag atagtatata
     3661 tggcaatttg cctgattaat tcagaaatat tagctttgaa gctctatctg gtccttaagc
     3721 cagatttatt aaaaaaggat agatgaacgg attcggcaga gggtttttaa cgttgcccac
     3781 agtagtcaaa aacctgataa tggtcaatgt gataatgctt ttggctacct gggtcattgg
     3841 cagtgcatta aattacaatc ttaccggcct gcttggcctg tactttccca aatcggaaag
     3901 tttcaagcca ttgcaaatcc ttacacacat gttcatgcac gcgggtttct ggcatctctt
     3961 cttcaatatg tatgcactgt tccttttcgg acagatactg gagaatgtat ggggaccgaa
     4021 acgattcctg atctattacc tggtttgcgg actgggggcg gcacttgtgc atgaaacggt
     4081 cattggcctt gaatataaca atcttataaa gaccctgagt ccggaacagg ttcataccgt
     4141 tatcaatgaa ggtgcttccc tgctgtcacg aaaccggcag tttgccgatc ccggcatgca
     4201 gaaactgcag ctattgctca ataccccgac cgtaggagct tccggggcag ttttcggaat
     4261 attgcttgca ttcggcgttc tctttccaaa cactcaactg atgcttcttt tccccccgat
     4321 tcccatcagg gccaaatact tcgtcctggc ttacggggga atagaactat acctggcatt
     4381 cacccagccc ggaagcaata tcgcccacgc agctcatctc gggggaatgc ttttcggata
//
LOCUS       DAOSPX010000275         4434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_17805_length_4434_cov_3.929436, whole genome shotgun sequence.
ACCESSION   DAOSPX010000275 DAOSPX010000000
VERSION     DAOSPX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17805_length_4434_cov_3.929436"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLZ75_11520"
     CDS             complement(<1..743)
                     /locus_tag="PLZ75_11520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOS73063.1"
                     /translation="MKAIKSETGAFREILLKTTFKTILILGMVLLILPSIAGNQAPGS
                     EEERLTLLFIGDIMGHDTQIRAAWNPETKSYNYDDQFRYVRPIIESCDFTAANLEVTL
                     AGPPYRGYPQFSSPPALAAACMNAGIECLFTANNHTADRGNRGILNTISRLDSMGIMH
                     TGIFRDRISADTLQPLIINKNGFSLAFLNYTYGTNGIPVPPPVVVNMLDKDRIAADIE
                     KARSLRPDLIVLALHWGVEYDTIPSEEQT"
     gene            819..2771
                     /locus_tag="PLZ75_11525"
     CDS             819..2771
                     /locus_tag="PLZ75_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOS73064.1"
                     /translation="MEAGKDSTGMRRFSAALSAAALAMVFGSLGTSPLYALRSLVSIG
                     SGNIDELLVYGGLSCIFWTLTLLGTIKYVFVSLEAGNNGEGGIFALFSLIRSKAPWTA
                     LLTMIAGAAFLADGVIMPAVTVTSSIEGFRLLKPSLPVFPIVLIIITVLFFVQQFGAN
                     RTGKFPGPVMAVWFVILAALGISGIMTHPGILRALSPAWAYHFIKEYPGGFILLGAVF
                     LSVTGAEMIYAGLGTFGSRNIRITWIFVKTALVLNYFGQGAWIMSNMKSASGINPFFS
                     MMPSWFLVPGIIIASAASIIASQSIITGSFSLMGEAVSLNLWPKIKVLHPTKSRVNIY
                     IPFVNWFLWISCSLTVIIFRESLDMTRAYGLAINIMEIMTTLLLSYYLLQKGISHRLV
                     LILLMVYLLAEGSFLAANLNKLSSGGWYTILITSVFFLVMYGWYFGRKLKNRYITFSD
                     LDKYLDLFRDLGRDESIPLYATNLVYIIKANREDQVESKVIYSIFHKQPKRAKTYWLL
                     HVDRVDEPNRFDYKVKQIIPGLLIRIDFRIGFKVDPRINLYFREVLEDMMEAGEISLE
                     SAYDSLKKHGLRGEFIFVLIDRIMLRDVKLSASENFILTLHLLVRRLGQPEEKALHLD
                     STSILVEKVPIIIDQPLSQRIRRVQA"
     gene            2873..>4434
                     /locus_tag="PLZ75_11530"
     CDS             2873..>4434
                     /locus_tag="PLZ75_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS73065.1"
                     /translation="MKKLLSAAIFLILFSGFAQSQAVAWKPAGDKILTPWAAEVNPLS
                     PLPEYPRPQMVRDSWKNLNGLWDYAITPSGSARPSVFDGKILVPFAVESALSGVNKSV
                     GPDNILWYRTRFNIPSDFRRKNVLLHFGAVDWKSEIYLNGALVGSHQGGYDPFTFDIT
                     AFIRGSGSQLLEVKVTDPVDRGPQPRGKQVLKPSGIWYTSVTGIWQTVWIEAVPKTYI
                     SSTKQVPDIDREILTLEVDVQEALPGDVVRVEAWDGRNMISDTEVAAGTKIELPVPDP
                     RLWSPDDPFLYDLKISVMRKGKVTDEVKSYFAMRKISLEPDADGWLRMMLNNRFVFQY
                     GPLDQGWWPDGLYTAPTDEALLFDIVKTKEMGFNTIRKHVKSEPARWYYHCDRTGMLV
                     WQDMPSGDLGGNQWNTRPGMEGGSDKIRTQESEMIFRTEWNAIMDYLFNYPCIVVWVP
                     FNEAWGQFRTEEITKWTMHKDPSRLVNSASGGNFHEVGHILDLHNYPGPVMAKPEVFG
                     TKQALVLGEFGGL"
BASE COUNT         1090 a          992 c         1132 g         1220 t
ORIGIN      
        1 ttggtctgtt cttccgaagg aatggtatca tattcgactc cccagtgaag tgcaagcacg
       61 atcaggtcgg gcctgagcga tctggctttt tcgatatcgg cagctatcct gtctttgtct
      121 aacatgttta caaccacagg cggcggaacg ggaattccgt tggttccgta ggtataattg
      181 agaaaggcga gtgaaaaacc gtttttgttt attataagcg gctgaagggt atcggccgaa
      241 atcctgtcgc gaaatattcc ggtatgcatg atccccatac tgtcgagcct ggagatggta
      301 ttcaggattc ccctgtttcc gcggtctgcc gtatgattgt ttgctgtgaa caggcattca
      361 atacccgcat tcatacatgc tgctgccagt gccgggggag agctgaactg gggatatccc
      421 ctgtagggtg gtccggccag ggtaacctcc aggttggcag cggtaaaatc acagctttca
      481 ataatgggtc tgacatatct gaactggtca tcataattat atgattttgt ttcagggttc
      541 catgctgccc ttatctgggt atcatggccc attatatccc ctataaagag tagggtgagc
      601 ctttcctcct cactgccggg agcctggttt ccggcaatgg agggcaggat cagtaacacc
      661 atgccaagta tcagtatggt tttaaatgta gttttaagaa gaatctcccg gaatgctccc
      721 gtttctgatt ttattgcttt catcacaggg caaagataat ttatttatac ctttgttctc
      781 cttttttgct gtttattgtg cttaaacaga tggaaatcat ggaagcaggc aaggatagta
      841 ccggaatgcg caggttttcc gctgcattgt cagcagccgc actggctatg gttttcggtt
      901 ccctgggaac cagtcccctt tatgcgctga gatccctcgt cagcattggt tcaggaaata
      961 ttgatgaact gctggtttac ggaggcctgt catgcatttt ctggaccctg acccttctgg
     1021 gcaccatcaa gtatgtgttt gtgtcacttg aggccgggaa caatggcgaa ggcggcatct
     1081 ttgcactttt ttccctgata aggagcaagg ctccctggac tgcccttctg accatgatag
     1141 ccggtgcggc ctttcttgcc gacggcgtga tcatgcctgc agttacggta acatcctcaa
     1201 ttgaaggatt cagattattg aagccctcat tgccggtttt cccgatagtt ctgataatca
     1261 ttaccgtact cttttttgta cagcagttcg gggcgaacag gaccggcaaa ttccccggtc
     1321 cggtcatggc tgtctggttt gtgatcctgg cggcactggg gatatcgggc attatgactc
     1381 atccgggcat tctcagggcc ctcagcccgg cctgggcata tcatttcata aaggaatacc
     1441 ccggcggctt catccttctg ggtgcggtat tcctttcggt aaccggcgca gaaatgatct
     1501 atgcgggtct tggaaccttc ggcagcagga atatcaggat tacatggata tttgtcaaaa
     1561 cagcacttgt cctgaattat ttcgggcagg gagcctggat aatgtccaat atgaagtcag
     1621 catcagggat caatcctttc ttttcaatga tgccctcatg gtttcttgtt ccggggataa
     1681 taattgcatc agctgcctcc attatagcca gccagtcaat aattacggga tccttcagtc
     1741 ttatgggaga agccgtttca cttaacctct ggccgaaaat caaagtactt caccctacaa
     1801 aatcaagggt caatatctat attccgtttg tcaactggtt cctctggatt tcctgcagtc
     1861 ttaccgtgat cattttccgg gaatcgcttg atatgaccag ggcatacggt ctggcaataa
     1921 atataatgga aataatgacc accctgctgc tgtcatatta cctgctgcag aaaggtatca
     1981 gccaccggct tgtgctgata ttgctgatgg tctatcttct ggctgaagga agctttcttg
     2041 cagctaatct gaataaacta agttcgggag ggtggtatac cattctgatc acttcagtat
     2101 ttttcctggt aatgtacgga tggtatttcg ggcggaaatt aaagaaccgt tatattactt
     2161 tttccgatct tgataaatat cttgaccttt tcagggatct tgggagggat gaatcaattc
     2221 ccctttatgc caccaacctg gtttatatta taaaggcaaa cagggaggat caggttgaat
     2281 cgaaggtgat atattcaata ttccataagc agcccaagag ggcaaagacc tactggcttc
     2341 ttcatgttga cagggttgat gaacctaaca ggttcgacta caaggtaaaa caaattattc
     2401 ccggtcttct gatcagaatc gatttcagga taggattcaa ggttgatcca agaatcaatc
     2461 tctatttcag ggaggtgctt gaggatatga tggaagcagg tgagatatcc cttgaaagcg
     2521 cttatgattc cctgaaaaaa catggtctgc ggggtgagtt catatttgta cttattgaca
     2581 ggataatgct tcgtgatgtc aagctgtcgg cttccgagaa tttcatactt actcttcacc
     2641 tgcttgtcag acggcttggc cagcccgaag aaaaagccct tcacctggat tcaaccagca
     2701 tcctggtaga gaaggtgccg ataatcattg atcagcctct gtctcaaaga atacggagag
     2761 tacaggctta actgccccga tgtcaagctt cttttctgga agggttttta ttctgaaaga
     2821 aattatgtta atttggggaa aggtaaatca ttattgtttc aagaggaatc ccatgaaaaa
     2881 attactaagc gcagcaattt tcctgattct tttttcagga tttgcacaat cacaggctgt
     2941 tgcctggaag ccggccggcg acaagatcct taccccgtgg gcggctgaag tcaatcccct
     3001 cagccctctt ccggaatatc ccagacctca gatggtacgg gattcctgga aaaacctgaa
     3061 cggattgtgg gattatgcca tcactccttc cgggagtgcc agaccttcag tctttgatgg
     3121 aaagatcctg gttccctttg ctgtggaatc ggcattgtcg ggtgtgaaca agagcgtcgg
     3181 acctgacaat atcctgtggt acaggaccag gttcaacatt ccttcggatt tcagaagaaa
     3241 aaatgtactt ctgcatttcg gggccgttga ctggaagtcg gaaatctatc tgaacggagc
     3301 acttgtaggt tctcatcagg gcggctatga tccctttacc tttgacatca ctgcctttat
     3361 caggggaagc ggctcacagc tcctggaggt taaagtgact gatccggttg accggggtcc
     3421 ccagcccagg ggtaagcagg tactcaaacc ctcggggata tggtatacct cggttacggg
     3481 aatatggcag actgtatgga ttgaagctgt tcccaagacc tatatatcat caacaaagca
     3541 ggttcccgat attgacaggg agatactgac actggaagtt gatgttcagg aagcccttcc
     3601 cggggacgtg gtccgtgttg aggcatggga cggacggaat atgatctcgg ataccgaggt
     3661 tgctgccgga acaaagattg agctgcctgt tccggatccc aggctgtggt cgcctgacga
     3721 cccctttctg tatgacctga aaatttccgt gatgagaaaa gggaaggtga cggatgaggt
     3781 taaaagttat tttgccatga ggaagatatc gcttgagccc gatgctgacg gatggctgag
     3841 gatgatgctt aataacaggt ttgtttttca gtatggtccg cttgatcagg gctggtggcc
     3901 cgacggattg tatactgcgc cgaccgatga ggcattgctg tttgatattg taaaaacgaa
     3961 ggaaatgggc tttaatacaa tacgcaagca cgtaaagtct gagccggcca gatggtatta
     4021 ccactgtgac aggaccggca tgctggtctg gcaggatatg cccagcggtg acctgggcgg
     4081 aaaccagtgg aacacccgtc ccggaatgga gggcggatca gacaagatac ggacacagga
     4141 gtcggaaatg attttccgta ctgaatggaa tgccataatg gattatcttt tcaattaccc
     4201 gtgtattgtc gtctgggtgc ctttcaacga agcctggggc cagttcagga cagaggaaat
     4261 aacgaaatgg accatgcaca aggatccgtc aagactggta aacagtgcaa gcggaggaaa
     4321 tttccacgag gtcggacata tcctcgacct gcataactat ccgggtcccg taatggcaaa
     4381 gcccgaggtt tttggcacaa aacaggctct tgttctcggg gaattcggcg gact
//
LOCUS       DAOSPX010000276         4430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_17823_length_4430_cov_3.876571, whole genome shotgun sequence.
ACCESSION   DAOSPX010000276 DAOSPX010000000
VERSION     DAOSPX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17823_length_4430_cov_3.876571"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..624
                     /locus_tag="PLZ75_11535"
     CDS             187..624
                     /locus_tag="PLZ75_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOS73066.1"
                     /translation="MEAHHILIVIGIVLFIAEIFLPGFIAGSLATGCFLAALASFLGL
                     AIKWQILIFSLGVLVTFFTIRPLMIKLEKRSGGKIKTNQEALIGRTGRVVETINNHEN
                     TGRISIDGDVWRSRSSDDSVIQEGTNVEIISISSIILMVKPLK"
     gene            632..1609
                     /locus_tag="PLZ75_11540"
     CDS             632..1609
                     /locus_tag="PLZ75_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOS73067.1"
                     /translation="MGAIIIFGAIALFVIIFAAAGFKIVQQAETMIIERLGRYNRTLS
                     SGINIIWPVIDKPREIIWRYVTEDEDGRKVVRFRTRNRIDLRETVYDFPRQNVITKDN
                     VNVEINALLYFQIMDPARAIYEIENLPDAIEKLTQTTLRNVIGELELDETLSSRDTIN
                     SKLRIILDDATNKWGVKINRVELQDINPPQDIKTAMEKQMRAERDRRAAVLEAEGQKR
                     SRILEAEGFKESEINKAEGEKQAEILRAEGLAQARVRVAEAEAKAITLVTDALKNKES
                     DPVKYLIAVRYIETLSEMVSGKDNKTIYMPFEATGVLSSLGGIKDILSK"
     gene            complement(1732..2853)
                     /locus_tag="PLZ75_11545"
     CDS             complement(1732..2853)
                     /locus_tag="PLZ75_11545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS73068.1"
                     /translation="MPVLILCLLISLLSPGNISGEKDRGKSAGKRIILRLEPGPGNPR
                     NSEGDFITLMDGRILFIYSRYTGGSGDDNDNAYLAGICSEDGGRTWSSESRMIIEQEG
                     KMNVMSVSLLRLQNGEIALFYLRKNSADDCIPMIRLSDDETKTWSEPQPCIMDRKGYF
                     VLNNGRVIQLRDGRLVLAVALHQEYGSSRWNNKGRLFSYYSDDNGRTWKAGSEVPNPG
                     GIVTQEPGLVELRTGNIMMFVRTDAGVQYLSYSADRGESWSPVERSDIRSPMSPASIE
                     RIPSTGDLLMVWNNNGGEDPEMKGKRTPLTIAVSKDEGKTWERIKNIEDDPDGWYCYT
                     AIHFTGRNVLLGYCAGNRPAGTGLAVTQITMLDLRWIYR"
     gene            complement(2891..>4430)
                     /locus_tag="PLZ75_11550"
     CDS             complement(2891..>4430)
                     /locus_tag="PLZ75_11550"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOS73069.1"
                     /translation="GSLNRMTAVAGQIKSKGTGGPGAPDIAWQTDSFIFGEENETRFT
                     PDFTFHTYRYMEISGLAYQPDPEDITGLFIHTDIQRPNHFSCSSGLLNSIQEITERTF
                     RANLVSVQSDCPAREKFGYGGDLNATCESFIYNFNMQSFYRKTILDWVDAMNDSSFVD
                     TAPYVGIAYCGISWESAFLITQYYLYLYYNDLDIVREYYEADKRWMEKVARIHPEGMV
                     HEGLSDHESLEPVPVELTGTCHYLQCAEIMQTFAGIMGDRDGEREYEQLAQRLRLLVR
                     AEFWDKPVKGKINRQTLFSALLYHGIVPDDEIEAAADSLRAAVRNGPSGHFSTGIFGT
                     KYILETLSEYASPGEVFAVVNSTEYPGWGHMISRGATTVWETWKESDNVYSNCHPMFG
                     TVSEWFYRWLGGIRPLPDEPGFKRFIISPAVPGGLDYVNCSWHSPRGEIVSDWKKQGT
                     DAVQYRVRIPGGSKALVDLQPSVVKRIEKVREGKPLQNIVPEGMSSGRFELAEGEYLI
                     SADL"
BASE COUNT         1215 a         1100 c          990 g         1125 t
ORIGIN      
        1 tcccgcgctt tccgacagaa cctttcagta tttagttccc tgattcaaac aatgaaaaac
       61 aggaaaatta tttccccgga ataagatctg tcagccggga acaaaccgat gtcttctttg
      121 tgaatattaa cgtatatttg agtcagagaa acaaaaaagt cagataatac aaaaacataa
      181 tttctcatgg aagcgcatca tatactgatt gtaataggaa tagtactgtt cattgccgaa
      241 atattcctgc cgggttttat tgcaggttcg ctggcaaccg ggtgtttcct ggcagcactt
      301 gcttctttcc tgggactggc gattaaatgg cagatattaa tattttcgct tggtgtgctg
      361 gtcacatttt tcactatcag acccctgatg attaaactgg agaaacgttc gggcggaaaa
      421 attaaaacaa accaggaagc cctgattggc aggacgggga gagtggtaga gacaatcaat
      481 aatcatgaaa ataccggccg tatcagtatc gacggtgatg tgtggcgctc cagatcgtca
      541 gatgactcag tgatacagga aggaacaaac gtcgagatca tcagtatatc cagtataatc
      601 ctcatggtta aacctttaaa ataaatcaca tatgggagca atcataatct tcggggcaat
      661 agccctgttc gtgataatct ttgcagctgc cgggttcaag atcgtccagc aggctgaaac
      721 gatgataatc gaaagactgg gaaggtacaa caggacactc tcttccggta tcaacattat
      781 ctggccggta atagacaaac caagagagat aatatggcgt tatgttacgg aagatgaaga
      841 cggacgtaaa gttgtcaggt tcaggacaag gaacagaatt gacctcaggg aaacggttta
      901 tgacttcccg cggcagaatg tgattacaaa ggataatgta aatgttgaaa tcaatgccct
      961 gctgtatttc cagatcatgg accccgcccg ggcaatatat gagattgaaa acctgcccga
     1021 tgccatagag aagctaaccc agaccaccct gaggaatgtc atcggggaac tggaacttga
     1081 tgaaacactc agttcaagag ataccataaa ctcgaaactc aggataatcc tcgacgatgc
     1141 aacaaacaaa tggggagtga aaataaaccg ggtggaactt caggatatca atcccccgca
     1201 ggatatcaag actgccatgg aaaaacagat gagggctgaa agggacagaa gggctgccgt
     1261 tcttgaggca gaaggacaga agagatcgag gatccttgag gcggaggggt tcaaggaatc
     1321 ggaaatcaac aaggccgagg gcgagaaaca ggctgaaata ctcagggccg aaggtcttgc
     1381 acaggccagg gtaagggttg cagaggcaga agcaaaagcc ataaccctcg ttaccgatgc
     1441 cctgaagaac aaggaaagcg atcccgtaaa atacctgatt gccgtcagat atatagaaac
     1501 attatcggaa atggtatccg gaaaggataa caaaaccatt tatatgcctt tcgaggcaac
     1561 gggagtgctc tcttcactgg gaggcataaa ggatatattg tcgaaataga attgcagttt
     1621 ctccaggact ttaaatggtt ccggaaaaat gacggcacca gggatatatt gccggcatcc
     1681 ttttcagttc ttcattcagg gttcaggcct tttgtgcgta ttagtgcccc gtcagcggta
     1741 gatccatctg agatcaagca ttgtaatctg agtgactgcc agaccggttc cggcaggccg
     1801 gtttcccgca caatatccca ggagtacatt tctgcccgtg aaatgaattg ctgtatagca
     1861 ataccacccg tcaggatcat cttcaatgtt tttaatcctt tcccaggttt tgccttcatc
     1921 cttcgaaacg gcaatggtaa ggggtgttct tttccctttc atttcgggat cttcaccacc
     1981 attattattc cagaccataa gcagatcgcc cgtggaagga atcctttcta ttgaagccgg
     2041 agacatgggc gatctgatat cacttctttc caccggactc cacgattctc ccctgtcagc
     2101 ggaataggac agatattgca caccggcatc ggtacggaca aacatcatta tattccccgt
     2161 ccttaattca accaggcctg gttcctgcgt cacgattccg ccgggattcg gcacttcact
     2221 gcctgccttc caggtacggc cgttatcatc cgaataatag ctgaacaggc ggcctttgtt
     2281 gttccaccgt gaactgccgt attcctggtg aagggccact gcaagaacaa gtctgccatc
     2341 tctgagctgg atgaccctgc cgttgttcag tacaaaatat cctttccggt ccataatgca
     2401 cggctgaggc tcactccagg tctttgtttc atcatcagac agcctgatca tcgggataca
     2461 gtcgtcagca gaatttttcc tcaggtagaa aagagctatc tcaccattct gaagcctgag
     2521 aagcgacacg gacatgacat tcattttacc ttcctgttca atgatcatgc ggctttcact
     2581 gctccatgtc cttcccccgt cttccgaaca tatccctgcc aggtatgcat tgtcattgtc
     2641 atctccggaa ccacccgtat accgggaata gataaacaat atccttccgt ccataagagt
     2701 aataaaatct ccttcactgt tcctcggatt tcccggcccc ggttcaagcc tcaggataat
     2761 tctttttcct gcagacttcc ccctgtcttt ttcccctgat atatttcccg gggaaagcag
     2821 ggatattaaa agacacagaa tcagtaccgg catgtcgcat cagtattatt cctgtcctgg
     2881 cggaaccgca tcacaggtca gcactgatca ggtattcacc ttcagccagc tcgaatctcc
     2941 cgctgctcat tccctcagga acaatgtttt gcaaaggttt tccttccctg accttctcta
     3001 tccttttaac aacggaaggc tgaagatcaa caagagcttt acttcccccg ggtatcctga
     3061 cacggtactg aacagcatcc gttccctgtt tcttccagtc agatacgatt tctccccggg
     3121 gtgaatgcca ggaacagttc acataatcaa ggccgcctgg aacagcaggg gaaatgatga
     3181 atcttttaaa tcccggctca tccggaagcg ggcggattcc tccaagccag cggtaaaacc
     3241 attccgacac cgtaccgaac atgggatgac agtttgaata tacattatcg ctttccttcc
     3301 aggtttccca gaccgttgta gcaccgcggc tgatcatatg accccatccc ggatattcag
     3361 tactgttaac aacggcaaaa acctccccgg gcgatgcata ctccgaaagt gtttccagaa
     3421 tatattttgt tccgaatatc ccggttgaaa aatgcccgga cggaccgttt cttactgcag
     3481 ccctgagtga atccgcggct gcttcaatct catcatcagg aacaatacca tgataaagca
     3541 aggcggaaaa tagtgtctga cggtttattt tacccttcac gggcttgtcc cagaactctg
     3601 ccctgacaag caatctgagc ctttgtgcaa gctgttcgta ttctctttct ccatccctgt
     3661 cgcccattat gccggcaaag gtctgcatta tttctgcaca ctgcaagtaa tggcatgttc
     3721 cggtcagttc cacaggaacc ggttcaagcg attcatggtc acttaatcct tcatgaacca
     3781 ttccttcagg atgaatccgt gcaacctttt ccatccagcg tttatccgcc tcatagtact
     3841 cccttacaat atccagatcg ttgtaataca ggtacaggta atactgggtg ataaggaaag
     3901 ctgattccca gctgatgccg cagtatgcaa taccaacata gggcgctgtg tccacaaagg
     3961 aggaatcatt catcgcatcc acccagtcca gtatcgtctt gcgataaaac gactgcatgt
     4021 tgaaattgta aataaaggat tcgcatgttg cattcaggtc acccccgtaa ccgaatttct
     4081 cccttgccgg gcaatcagac tgaacgctta caagatttgc cctgaaggtc ctttccgtta
     4141 tctcctgtat tgagttcagg agaccggaag aacaggaaaa gtgattcggc ctttgaatat
     4201 ccgtatggat gaagaggccg gtaatatctt ccggatccgg ctgataagcc agacccgaga
     4261 tttccatata cctgtaggta tgaaatgtaa aatccggtgt aaaccgggtt tcgttttcct
     4321 ctccgaaaat aaaactgtcg gtctgccatg caatatcagg agcacccgga cctccggttc
     4381 ccttgctctt gatctgaccg gcaacagcag tcatgcgatt aaggctcccg
//
LOCUS       DAOSPX010000277         4426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_17839_length_4426_cov_3.792038, whole genome shotgun sequence.
ACCESSION   DAOSPX010000277 DAOSPX010000000
VERSION     DAOSPX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17839_length_4426_cov_3.792038"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PLZ75_11555"
     CDS             <1..1188
                     /locus_tag="PLZ75_11555"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOS73070.1"
                     /translation="KLRIGLFGYGCVGQGLHDVLNASSGFRADIVKICVRDREKERRI
                     PMSYFTFDKNDILDNESINLVVELIDDAEEAFNIVSAAVRKGKNVVTANKLMVANHFA
                     ELVEMQEKYGVSVLYEASAGASIPIIRNLEEYYDNELLHSVRGILNGTTNYILTRMYS
                     EGLDYNEALNDAKAKGFAESDPRLDVEGYDAKYKICIITGHAYGLIINPQEVFNYGIS
                     SISQDDIRYAREKGLKIKLLSYAAKVNENTITSYVLPRFISTDKHLYYVDNEYNGIIA
                     EAAFADKQFFSGKGAGGHPTGSAVLSDISANSFGYRYEYKKWAQGTITNYTRDHKLEI
                     YLRYSDAKDLELFHFDSISEDFKSLDYKYVIGIINLEQLYSLRDRLSGMDIFIAGTGR
                     KIQ"
     gene            complement(1360..2964)
                     /locus_tag="PLZ75_11560"
     CDS             complement(1360..2964)
                     /locus_tag="PLZ75_11560"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS73071.1"
                     /translation="MKRRDFIKLSSSAGIAGMAGRRFDSFAVSGKERMTGFDLHPFIR
                     EHPEAVFVCLTSVKEKTDAGAIYDAGFKLSGEMFVKTTEGRGFSNSTRIVCKPNWTAK
                     RIDPADPTSHLGITTDNNFIEGFLNGVKATGPGDIYLRECAAPRSWDVNGYTSMCARN
                     NFNLRDLSSKDFWDLDDEIIFKKVDGTVFREIGFMAPVNAPDTFLINLAKFKAHSMGI
                     TGAIKNLQGTTGRRFHQFCGGYRDVFKLYDKRYHPFFQPDYLARIAELHKKHIEAGIP
                     RWDTEIDNPPFRGGGGGFYMEHWVQRMLDAFSATPTGINIVEGIYGRDGDGFAAGPHE
                     GKAKDYMSNNIIFGTDAFRVDIILHWLAGHEPGNFGLFHIEMERGFSDVLDPFDIPVY
                     LWENGSARKVDLDTLKRTPLLTPYLRKNTRGDDEERYHMCDEPFDYEAWKSTGKIIRY
                     EPSIKAIGTDAADKIVMDMKVPEKGDVYVEILNSDGEVVWRMYADDLEPGVHQVVWDG
                     FSSPGLYTTYVKGMEWDAENEMVIYT"
     gene            complement(3259..>4426)
                     /locus_tag="PLZ75_11565"
     CDS             complement(3259..>4426)
                     /locus_tag="PLZ75_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS73072.1"
                     /translation="GPGLHPYHHAATEASASVDMLKAAMQYCNSAPDIKAKPNGSLYL
                     EGYSQGGHAALATQEELQRNPVPGLILQKTVAGSGAYSLSLVQKNFVFDHADYASPSF
                     LPYLLLGYQEVYGNMYGNLNQVFVQPYSTAMPGLFDGSLTVNEINLQLPAQWQSMFNP
                     TYLWNLRYSYLHPVNSALRKNDLINWKPQSDLHLYYCTCDELVDNNNSLLAYLSFILK
                     GSNRVTTAPLGPFKHAECAPFVMLLAKVKFDCDSHANPCGINLLSLLKSDTDDELVQF
                     SQAMEESYQAPAVEDLLQDNILASYLSQEIPQEKNKLHVYPNPANDIVNVQLSSEMAG
                     KAIISLFDMQGRRIREENYTGNLMIIDVKPLPAGIYKVVVSGDINLSQTLVVSK"
BASE COUNT         1254 a          992 c          954 g         1226 t
ORIGIN      
        1 aaactacgga tcgggctatt cggctacggc tgcgtcgggc agggattgca tgatgttctc
       61 aatgccagca gcggcttcag ggctgatatt gttaaaatat gtgtcaggga ccgggaaaag
      121 gaacggagga taccgatgtc gtacttcact ttcgacaaga atgacatcct cgacaatgag
      181 tcgatcaatc ttgtggttga acttattgat gatgccgaag aggctttcaa tattgtttcg
      241 gctgccgtaa ggaaaggcaa aaatgttgtc acagccaata agctgatggt tgcaaatcac
      301 ttcgcggaac tggttgaaat gcaggagaaa tacggtgttt cggttttgta tgaggcaagt
      361 gccggggcaa gcattcccat aataaggaac cttgaagagt attatgacaa tgaactcctg
      421 cattcggtaa gagggatact gaacggaacc accaattaca ttctcaccag aatgtacagc
      481 gaaggccttg attacaatga ggcactgaat gatgcaaagg caaaaggctt tgcggaatca
      541 gacccccgtc ttgatgtgga aggatacgat gcgaaataca agatatgcat tatcacgggt
      601 catgcctacg gactcatcat caacccccag gaggtattca actacggcat cagttccatt
      661 tcgcaggatg atatccggta tgcaagggag aaaggactga aaatcaaatt attgtcttat
      721 gcagcaaaag taaatgaaaa tactattacc agttatgtac tgccaagatt catcagtacc
      781 gataagcatt tgtattatgt tgacaatgaa tataacggga taatagcaga agcagccttt
      841 gccgacaagc agtttttcag cggaaagggt gcaggcggtc atcccacggg aagtgcggtt
      901 ctttcggata tttcggccaa ttcattcgga tacaggtatg aatataaaaa atgggcgcag
      961 ggaaccataa ccaattacac acgtgatcac aaactggaaa tatatctcag gtattcagat
     1021 gcaaaggatc ttgaactttt tcattttgat tcaatatccg aagattttaa aagtctggat
     1081 tacaagtatg ttatcgggat tataaatctt gagcagcttt attctctcag ggacaggctt
     1141 tccgggatgg atatattcat tgccggaaca gggaggaaaa ttcagtgata cgggaacagg
     1201 cgaaaagtct tttataaaag actaacagac atatattcag gcagacagac cacacggaaa
     1261 gctttggtct ttccatgcag gctatgtgtc catgcggctt attcagtccg gggaacaggt
     1321 tccccggata tctgaacaag cgctgactgt ttaaacaaac taagtataaa taaccatttc
     1381 attttccgca tcccattcca tgcctttaac atatgttgta taaaggcccg gggatgaaaa
     1441 tccgtcccat accacctggt gaaccccggg ttccagatca tctgcataca tcctccaaac
     1501 cacctctccg tcactattga gtatttcaac ataaacatcc cccttttccg gaactttcat
     1561 atccattacg attttatctg ctgcatctgt tccgattgct tttatggaag gctcatagcg
     1621 gatgatcttc ccggttgatt tccaggcctc ataatcaaat ggctcatcac acatgtgata
     1681 tctttcttca tcatccccgc gggtattctt ccggagatac ggagtgagca aaggagtacg
     1741 tttcaatgtg tcaagatcaa ctttcctggc tgaaccgttt tcccacagat aaaccggaat
     1801 atcaaaagga tcgagtacat ctgagaagcc tctttccatc tctatatgga acaggccaaa
     1861 gttccccggt tcatgaccgg caagccagtg cagaatgata tcaaccctga aggcatctgt
     1921 tccgaagatt atattattgc tcatataatc ctttgcttta ccctcatgag gtccggctgc
     1981 aaatccgtcg ccatcacgcc cgtatatacc ttcaactata tttattccgg tcggtgttgc
     2041 actgaatgca tcaagcatcc tttgaaccca gtgctccata tagaaaccac cacctcctcc
     2101 tctgaaagga ggattatcta tttcggtatc ccatcttggt atccccgctt caatatgttt
     2161 tttatgcaat tctgctatcc tggccagata atccggctga aagaacggat gatacctctt
     2221 gtcatataat ttaaaaacat cgcggtatcc tccgcaaaac tgatgaaatc ttcttcctgt
     2281 tgttccctgg aggtttttta tagctcccgt tatacccatt gagtgggcct tgaattttgc
     2341 cagattaata agaaaagtat caggtgcatt taccggagcc ataaacccga tctccctgaa
     2401 caccgtcccg tcaacttttt taaatattat ttcatcgtcc agatcccaaa aatcttttga
     2461 tgaaagatcc ctgaggttaa aattgtttct cgcacacatg gaggtatagc cattgacatc
     2521 ccacgagcgg ggagccgcgc attctcttaa atatatatcc ccgggacctg tggcttttac
     2581 tccattgagg aaaccttcaa taaaattatt atccgtagta attccaagat gggaagtcgg
     2641 atcagccgga tcaatacgct tggctgtcca gtttggcttg cagactatcc tggttgaatt
     2701 tgaaaaacct ctgccctccg tggtttttac aaacatctcg ccggagagtt taaaacctgc
     2761 atcataaatt gcgccggcat ctgttttttc ttttacggat gtaaggcaga caaatactgc
     2821 ttccggatgc tcccggataa acggatgcag gtcaaatcca gtcatccttt ccttaccgga
     2881 aacagcgaaa gaatcaaacc tcctgcccgc cataccggct attccggcag atgatgataa
     2941 ttttatgaaa tcccgcctct tcataattaa tctgcttata tatattttct gctacagacc
     3001 ataaatcctt aaagaaagaa gattattcaa atttataatt attcaagttc cgcagttcct
     3061 tataatgttg aaaccaaata gaaattatta aaaaatgcag cacggtttgt gtgttaatat
     3121 gctgtttttg agcatattaa caacgttgtc caaaacgtac aaccatgctg caaagtaaag
     3181 atatcaataa ttacggaatt taaaagcgct ttcacccaca ggtacctgaa aaagtaaaag
     3241 cagatcggcg gaatgatttt attttgatac taccagggtc tggctcaggt ttatgtcacc
     3301 cgaaacaact actttatata ttcctgcagg cagtggctta acgtcaatta tcatcaggtt
     3361 gccggtgtaa ttttcttccc ggatccttct tccctgcatg tcaaacaggc ttattatcgc
     3421 ttttcctgcc atttctgacg ataactgaac atttacaatg tcatttgccg gattcggata
     3481 aacatgcagc ttattcttct cctgaggaat ttcctgagaa aggtatgatg caaggatatt
     3541 gtcctgcagc aggtcttcca cggcaggagc ctgataggat tcctccatag cctgggaaaa
     3601 ctggacaagt tcatcatctg tgtccgattt cagcaggctc agcagattta tgccgcaggg
     3661 attggcatga gaatcacagt caaattttac cttggccagt aacattacaa aaggagcaca
     3721 ttcagcatgt ttgaacggac cgagcggagc agtagtaact ctgttgctgc ccttgagaat
     3781 gaatgaaagg taggctagca gcgagttgtt gttgtcaacc agttcatcgc atgtacagta
     3841 gtagagatgc aaatcgctct ggggcttcca gttgattaga tcgttttttc tgagtgccga
     3901 attaacagga tgtaaataac tataccggag gttccacagg taggtgggat tgaacatgga
     3961 ctgccattgt gcaggcagct gcagattaat ctcatttaca gtgagcgagc cgtcaaaaag
     4021 ccctggcatt gcggtactgt acggctggac aaacacctga ttcaaattgc cgtacatatt
     4081 tccatatact tcctgatagc ccaacagcag ataaggcaga aacgagggac tggcatagtc
     4141 ggcatgatca aaaacaaaat ttttctgaac gagtgataaa ctataggcac cgctgcctgc
     4201 cacagtcttt tgcagaatga gccccggaac aggattccgc tgcagttctt cctgtgtagc
     4261 cagtgcggca tgcccgccct gtgaataacc ttcaagatag agactgccgt ttggtttggc
     4321 cttgatatcg ggagcactgt tgcaatactg cattgcagct ttcagcatgt ccacgctggc
     4381 tgatgcttct gttgcagcat ggtggtaggg atgcagcccg ggaccg
//
LOCUS       DAOSPX010000278         4377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18071_length_4377_cov_4.549977, whole genome shotgun sequence.
ACCESSION   DAOSPX010000278 DAOSPX010000000
VERSION     DAOSPX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18071_length_4377_cov_4.549977"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLZ75_11570"
     CDS             <1..232
                     /locus_tag="PLZ75_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73073.1"
                     /translation="TYSVVEKPAGTVTSLGKGDGSYEFRCETSGNEILISAHLKIDNT
                     FFEASEYSRIREYYSGILQKQAELIVMSKNEE"
     gene            239..2122
                     /locus_tag="PLZ75_11575"
     CDS             239..2122
                     /locus_tag="PLZ75_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing transglutaminase
                     family protein"
                     /protein_id="HOS73074.1"
                     /translation="MKYLLTILMAAITSNVFSQLYSFDSIPDNLKNRASAVVRTEQCL
                     FTIKGPGNAVMKIKKAVTLLNENATAYRLLQIMYDKYSKVSGIRGMIYDQKGNIAEAL
                     GPSDVFDISAISGGTFYSDDRIKLLYFPIYKYPYTIEYEYEITFSSLLNYPSWNFQDA
                     PDVSVQKSGIQYVVPSGMKLRYYDEYLKNPVDSVILDNKKIYTWQEENIPAYESRDVS
                     IRPVYHIPSVNAAPLDFEYAGFRGSMRSWKEFGNWVYEINRDRDELPQSEIDIVRKLV
                     SEEQGTREKIKRIYEYMQLKTRYVSIQIGIGGYRTAEASAVARNGFGDCKALVNYTYS
                     LLKAAGIKSFYTLVRQSSVSDINKDFVNNQFNHAILCVPQAEDTIWLECTSQTYPFNY
                     LSSSTSNKNALLITPEGGIMAKTPSFKKEQNLIRREGNFSISTQGVSSGRMSSIYSGH
                     HYESATFRYAMQSEDEIKRSLYSGMRFNDLDISGVKYSERKTENPSAELECQLAIKNF
                     STSDASRMYFNPVISVQDYFQEKPVHLKVVLASVTSDSIVYNIPENYVVEYMPPDIDI
                     ENEFGRFVCKFEKEYQVLIFRRIFELNESDISAEKYSSFRTFINTIAKADREKIVLLR
                     TGG"
     gene            complement(2208..3944)
                     /locus_tag="PLZ75_11580"
     CDS             complement(2208..3944)
                     /locus_tag="PLZ75_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73075.1"
                     /translation="MTIDFSDKKTLEKYSSAFTLSDMEIFIFPELFYPLVMANIMSPI
                     IWKWREDPWFADIREKSFNYKVNRIKQYIIDNYVFNLDLATWGLTTKTREIERFRDFF
                     DVEVLRRSNALFGYEGDKYYFDIDIRKHFGLDKFNSDVIPYWKTETVEAMTAFRHKEN
                     FTTGAGECVSLSALYAAAMFIVAGIPLEKIFLIATPLHSQNFITEKEGVITNNRRIVT
                     KNMWFNGTSLSEKARRALENERVTMVSHISGYMHTIYRDATINRKCYKWFSGKLRDFL
                     STELTDRVFINFLRFKSKYKSLFQVECTCSGISYYISLEKMLEYEHTSKFSASPDTRE
                     KLIGEIEGDEFHLSPLPGKIILNRAEEILGTHKNSDLKELEILFTGLNGEIKKDMIRE
                     MFEDLAKFITTRPNLPEEDKNYIDSVVPEISVNDSREQIIEKIHGHADRSEMALLTLY
                     AYRQMDKADWLPFVKAAIERNPTCFGDLNGKSIREVYEILTAMPDESVYDGKRLALPD
                     EIWNFKRGDGLEKAFLLAVFITGTDPAAEINIVSADETVRLSCSGNAYLFRTKKNFRK
                     HIRISGSNYEIS"
     gene            complement(3978..>4377)
                     /locus_tag="PLZ75_11585"
     CDS             complement(3978..>4377)
                     /locus_tag="PLZ75_11585"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOS73076.1"
                     /translation="AIIKPNAVRTGKTGPILAMINEAGFEISALKMTSLTVPQAEAFY
                     NFCQEEPVFESLVEFMSSGPVVAMILRRENAVEEFSRLIGDPDPEFAEPGTIRKQYAV
                     NLLMNAIHGSESEEDARRESEFFFSEIEKF"
BASE COUNT         1242 a          909 c          919 g         1307 t
ORIGIN      
        1 aacctattca gttgtggaaa agcctgccgg caccgtgaca agtcttggga agggggatgg
       61 cagttatgaa ttcagatgtg agaccagcgg caatgagatt ctgatttcag ctcatctgaa
      121 aattgacaat acattcttcg aagcatctga atattcccgg atcagggagt attattccgg
      181 gattctgcaa aagcaggcag aattgattgt gatgtccaaa aatgaagaat gatataaaat
      241 gaaatacctg ttaaccatat tgatggctgc aattacatcc aatgtatttt cccagcttta
      301 ttccttcgat tccattcctg ataatctgaa aaacagagcc agtgcagttg tcagaactga
      361 acagtgtctc tttaccataa aaggaccggg caatgctgta atgaagatca aaaaggcagt
      421 cacacttctc aatgaaaatg caaccgctta cagacttttg cagattatgt atgacaaata
      481 ctcaaaagta agcggtatca gagggatgat atatgatcag aaaggtaaca ttgccgaagc
      541 actcggacct tcggatgttt ttgacataag tgcaataagc ggcgggacat tttattcgga
      601 cgacaggatc aagctgctct atttcccgat atataaatat ccctatacca ttgaatatga
      661 atatgaaatt actttttcca gtctgttaaa ctacccttcg tggaattttc aggatgctcc
      721 ggatgtatct gttcagaaat caggtataca gtatgttgtt ccatcaggaa tgaaactgag
      781 gtattatgat gagtatctga agaatcctgt ggattcggtg atactggata ataaaaagat
      841 ttatacctgg caggaagaaa atattccggc atatgaaagc cgggatgtct ccatccggcc
      901 ggtttatcat ataccctcag ttaatgcagc ccccctagat tttgaatatg ccggattcag
      961 gggatcaatg cgttcatgga aggaattcgg caactgggta tatgaaataa acagggacag
     1021 ggatgaactt ccacagtcag agattgacat cgtccgaaaa cttgtttcag aagagcaggg
     1081 aacccgggaa aagataaaac ggatttatga gtatatgcaa ttaaaaacca ggtatgtttc
     1141 aattcagatt ggaatagggg gttaccgtac agctgaagct tcagctgttg cccggaacgg
     1201 attcggtgat tgcaaggccc tggtcaatta tacctattca cttctgaagg cagccggtat
     1261 taaatcgttt tatactcttg taaggcaaag ttcagtatct gatataaaca aagattttgt
     1321 caacaatcag tttaaccatg caatattgtg tgtgcctcag gccgaagata caatatggct
     1381 ggaatgcacc agtcagactt atccgtttaa ttatttgagc agcagcactt ccaataagaa
     1441 tgctcttctg ataactcctg aaggtggtat aatggccaag actccttctt ttaaaaagga
     1501 acagaatctt attcgcaggg aaggtaattt ttccatcagt acacagggag tatcatcagg
     1561 aagaatgtcc agtatttatt caggacatca ctatgaatcg gcgacattca gatatgccat
     1621 gcagtcggag gatgaaatca aacgttccct ctactcaggc atgcggttca atgaccttga
     1681 tatatcaggg gttaaatatt ctgaaagaaa gacggaaaat ccatcagcag aacttgaatg
     1741 tcagcttgca ataaaaaatt tcagtacttc tgatgcttca aggatgtact tcaatcctgt
     1801 gatttcagta caggattatt ttcaggagaa acccgtgcac cttaaagtag tattggcctc
     1861 ggttacatct gattccattg tatacaacat tccggagaat tatgttgttg aatatatgcc
     1921 tccggatatt gatatcgaaa atgaattcgg cagatttgtc tgcaaattcg aaaaggaata
     1981 tcaagtattg atattcagaa gaatatttga attaaatgag tctgatataa gtgcagaaaa
     2041 atatagcagc ttcaggacat ttataaatac aatagcgaaa gctgacaggg agaagattgt
     2101 tctgttaaga accggtggat aaaaaatttt tcagcagttt cctggtccgg ccggcaaatc
     2161 aggtttcagc acagaatctt gatcggtgga tgatattttt ccgcggatta actgatttcg
     2221 tagttacttc cgctgattcg gatatgttta cggaaattct ttttagttct gaatagataa
     2281 gcattgccgg aacagctgag tctgacagtt tcatcagcgc tcacgatgtt tatctctgct
     2341 gcaggatccg tgcctgttat gaaaacagcc agcagaaatg ccttttccag tccgtcaccc
     2401 cttttgaaat tccatatctc atcgggaaga gccagcctct ttccgtcata aacagattcg
     2461 tcaggcatag cggtgagtat ctcataaacc tcccttatac ttttcccgtt cagatccccg
     2521 aagcaggtgg gattacgttc aatggccgct ttcacgaaag gaagccagtc agccttatcc
     2581 atctgcctgt atgcatacag tgtgagcaat gccatctcgg acctgtccgc atgcccgtgt
     2641 atcttttcaa ttatctgctc acggctgtcg ttaaccgaaa tttccggcac aaccgaatct
     2701 atgtaattct tatcctcctc aggcagatta gggcgggttg tgatgaattt tgccagatct
     2761 tcaaacattt cccttatcat atccttcttt atttctccgt tcagcccggt gaaaagaatt
     2821 tcaagctctt tcaggtctga attcttatgc gtacccagta tctcttcggc cctgttaaga
     2881 ataattttgc ccgggagcgg gctcagatga aattcatccc cttcaatttc cccgataagc
     2941 ttttcccttg tatcaggcga ggcactgaac ttgctggtgt gctcatattc cagcattttt
     3001 tccagcgaga tataataact gattccggag caagtacatt caacctgaaa gagagattta
     3061 tattttgatt tgaatctgag aaagtttatg aatacacggt cagtaagctc agtgctcaga
     3121 aaatctctca gctttccgga aaaccactta taacacttcc tgtttattgt tgcatccctg
     3181 tatattgtat gcatatagcc ggaaatatgg ctgaccattg taactctttc attttccaga
     3241 gcccgtcttg ccttttctga aagggaagtt ccgttgaacc acatgttttt tgttactatt
     3301 ctgcggttgt tggttattac tccttctttt tccgtgatga aattctgtga atgcaggggt
     3361 gtggctatca ggaatatttt ttccagggga atacctgcaa cgataaacat tgccgcggca
     3421 tagagtgccg ataatgatac acattctcct gctcctgtcg tgaaattctc cttgtgcctg
     3481 aaagccgtca tggcttcaac ggtttcggtt ttccagtacg gaatgacatc tgaattgaat
     3541 ttgtcaagtc cgaaatgctt gcggatatct atatcgaaat agtatttgtc tccttcatag
     3601 ccgaacagtg catttgagcg cctgagaacc tcaacatcga aaaagtcccg gaaccgttca
     3661 atttcccttg tcttggtcgt aagtccccag gttgccagat caaggttgaa tacgtagttg
     3721 tcgattatgt attgctttat tcggtttacc ttgtaattga agctcttttc cctgatatca
     3781 gcgaaccatg gatcttccct ccatttccat attatgggcg acatgatgtt tgccataacc
     3841 agggggtaga atagttcggg aaatatgaat atctccatat cggagagggt gaaggccgat
     3901 gaatattttt caagggtctt cttgtcggaa aaatcaatag tcatttgaga attcattgga
     3961 aagttaaaaa ccagggatca gaatttttct atttctgaaa agaaaaactc actttccctc
     4021 cgggcatctt cttcactttc ggaaccatgt atggcattca ttagaaggtt tacagcatac
     4081 tgcttcctta ttgttccggg ttccgcaaat tctggatcgg gatcccctat aagcctgctg
     4141 aactcctcaa ccgcattttc ccttctcagt atcattgcca caaccgggcc ggaactcata
     4201 aattccacaa ggctttcaaa aaccggctct tcctggcaga agttgtaaaa ggcttcagcc
     4261 tggggcactg tcagagatgt cattttcaat gccgaaatct caaatccggc ttcgtttatc
     4321 atagccagta tcggacctgt tttgcccgtt cttacagcat tgggttttat aatcgca
//
LOCUS       DAOSPX010000279         4375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18085_length_4375_cov_4.109954, whole genome shotgun sequence.
ACCESSION   DAOSPX010000279 DAOSPX010000000
VERSION     DAOSPX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18085_length_4375_cov_4.109954"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /gene="ssrA"
                     /locus_tag="PLZ75_11590"
     tmRNA           <1..99
                     /gene="ssrA"
                     /locus_tag="PLZ75_11590"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            470..712
                     /locus_tag="PLZ75_11595"
     CDS             470..712
                     /locus_tag="PLZ75_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73077.1"
                     /translation="MKRLAKIIIFSFLISVSMTGCYAGKRNLRELGGLMMLDNTQQKR
                     NRAFYSGHNKKARKNVHSKYRKAPEKSYKKTFRAFR"
     gene            888..1193
                     /locus_tag="PLZ75_11600"
     CDS             888..1193
                     /locus_tag="PLZ75_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73078.1"
                     /translation="MKKSQDTDKVKLLNFSRPIRNALLKGEIISGVIKKHNGDLIAKY
                     TKTVINNIRYYGIWEKDLLEGKWHLLHYGHKDDVESEWKSFTDFWKRQGDRISPGIA"
     gene            complement(1201..1854)
                     /locus_tag="PLZ75_11605"
     CDS             complement(1201..1854)
                     /locus_tag="PLZ75_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOS73079.1"
                     /translation="MRHNLFIFLISFLFLCSGQSCATFKNVGELIGKSTRRQLADNES
                     PDNYSESITGFLGIVKHTVCGSEKWADPLGIQGGIIYPFYRINDLIDLRIEANISMQG
                     AKWEESGIRGRTNLLYVNTPVVARYLTDFGFYAEGGIQSGFLISARKYEGTVENYKKN
                     FRSVDLSIPLGIGYEFRNNFGIGIRIIPGFTDITRYSSAYDRNFVMAFRGTYIFSFK"
     gene            2283..3494
                     /locus_tag="PLZ75_11610"
     CDS             2283..3494
                     /locus_tag="PLZ75_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS73080.1"
                     /translation="MVFLMMLYSINTTAQIQGCTDPPALNYNSSATINDGSCIYSTTS
                     VSPDISLNLDNRLVETSGLVIWNDRLWTHNDSDDINLYSLDTVRGNIIQSYTLEGTVN
                     RDWEEISQDRDFIYIGDFGNNSSGNRTDLMILRISKNSIVNERLIIDTIRFSYPDQTD
                     FSPASSNYTDFDCEAFIVSADSIYLFTKQWISQKTTCYSLPKIPGTHTAVRKNTLDVR
                     GLITGAVYLESKKLIALCGYTPLLEPFIFLLYDFNNPDFFSGNKRRIDISLPFHQTEG
                     ITTSDGLKYYITNEYFQFLNIRQKLHIVSLDPFLQHYLKPGNPSVDRPGKNDDYKVYL
                     VREDNCLIVKKKENDRNEDYSLISLSGQTVLRGKLYGKENVINISHLTSGLYLFRIGK
                     NSIHPVKLIKI"
     gene            complement(3661..4236)
                     /locus_tag="PLZ75_11615"
     CDS             complement(3661..4236)
                     /locus_tag="PLZ75_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73081.1"
                     /translation="MKKFIGFLLLIGLFSPGLLSQQSTSVTNPSFRYQATPGFVNISE
                     INGGSGSYYCDPAVSKYYFGLTNVFGYQFSRSFMGGIGLGYFQLDKGFYLPLYLENKY
                     SFYLKSFTASAILDGGVLIDHSAPLDDSKIFLNPGAGISRTITPNLEISFSAGYMIQN
                     RTTLSRVGFLNCKAGLIYRKNPFRMFKSGKN"
BASE COUNT         1453 a          884 c          823 g         1215 t
ORIGIN      
        1 ctgcagctgg caggtcacga aaaacaagca gaactaagca tgtagaaagc gtatggtctc
       61 catgtctgga cgcgggttcg actcccgcca actccactac gaaccacatt cgtaacgatt
      121 gtggtttctt tttttccctc ctgatccatt aagaaatctg gcataataag tcgccgtgaa
      181 tgaattccgt tagcagaatc agggataaac cggaaaggat ctttattatt tatctgcaca
      241 acggaggaat tcaggctttt ttaaacttcc tgacacacga ctacagtgtg tttatctggc
      301 atacatcagg ataagatatt attaccggaa gccataagag tgttttgctg aaagaaactg
      361 aggaacattt tccctaacca aacgtaaaag tactttgaaa gaatcatgaa atgaccatat
      421 tatgcattaa accctgatta gcagaacggt ttcttaaaaa gaaggcatta tgaaaagact
      481 ggcaaaaatt atcattttca gcttcctgat ctcggtttca atgacaggat gttacgccgg
      541 caaaaggaat ctcagagaac tcggggggct gatgatgctt gacaataccc agcaaaaacg
      601 gaacagggct ttttattccg gacataataa aaaagccagg aaaaatgttc acagtaaata
      661 caggaaagca cctgaaaaaa gctataaaaa gacattcagg gctttccgtt aataaaaacg
      721 ctgcattgaa catagttccg ggcgacaggc tgccgggccg gaatataatc cgctatcaga
      781 gatcaggtac cctgatcttt tttgtattga aacatttccg gtcattttat cgtattacta
      841 tgtcagaaag tcaataatat caacatattt aaccaaaacc tgaaaatatg aaaaagtctc
      901 aagatacgga taaagtgaaa ttgttgaatt tcagcagacc aatccggaat gctttgctaa
      961 agggggaaat aatttccggc gttataaaga aacataatgg cgacctgata gccaaatata
     1021 ccaaaactgt catcaacaat atccgttatt acggtatctg ggaaaaagat ctgctggaag
     1081 gcaagtggca tttattacat tacggccata aagacgacgt cgaatccgaa tggaagagtt
     1141 ttacagattt ctggaaaaga cagggagaca gaatctcacc cgggatagcc taagccggta
     1201 ctatttaaaa ctgaaaatat aggttcccct gaatgccata acaaaattcc tgtcataagc
     1261 tgaagaatat cttgtaatat cagtaaaacc gggaattatt cttataccga taccgaaatt
     1321 attcctgaat tcataaccaa ttcccagcgg aatggaaaga tccactgagc ggaaattttt
     1381 cttgtagttc tccaccgtcc cctcatattt tctggcactt atcaaaaaac cactttgtat
     1441 tcccccctct gcataaaatc cgaaatccgt cagatacctg gcaactaccg gagtgttgac
     1501 atatagaaga ttagttctcc cccttattcc tgattcctcc catttggcac cctgcatact
     1561 tatatttgct tcaattctca gatcaattaa atcatttatc ctgtaaaaag gataaattat
     1621 tcccccctga atcccaagag gatctgccca tttttcactg ccgcagacag tatgttttac
     1681 aattcccagg aatcctgtta tggattctga ataattatcc ggagattcat tgtccgccag
     1741 ctgtctgcgc gtagatttcc cgataagctc cccgacattt ttaaatgtcg cacatgattg
     1801 accggaacat aaaaagagga aagaaatcag aaaaataaat aaattatgcc tcatgaaaac
     1861 aaacagaact gaaggtgata tttgaacaat ctgtcaaagt taaatttatt ttcggaacat
     1921 actgattatt attgcagatt gcatttcctc tcctaaatat cccgctgccg acaccatagt
     1981 cctgtccgaa aatgaattcc accggaaagg acagagtcat aagtatatta atattagtta
     2041 tttttgtaac tgtacagttg gaataacaat gctgctgagc aggtgcaata ctgcagagat
     2101 aatgcattat tgcagaatcg ggacaatatg gcttagtaag tacaatattg cctggctgga
     2161 atgccacttc atagtcagaa cctgtactgc tgcattgaaa taataccgta cataaaaaca
     2221 attttgtaca gctaaaagaa cagtaaatgc aaatttgcag gcaaaccggg aaattattca
     2281 ttgtggtatt tctaatgatg ttatacagca ttaataccac agctcaaata caaggctgta
     2341 ctgatccgcc agcgctcaat tataattctt ctgcaacgat aaatgatggc agctgtattt
     2401 attccaccac ctcggtaagc ccggacatta gcctgaacct ggacaacagg cttgtcgaaa
     2461 catcaggact ggtaatctgg aatgaccgct tatggaccca taatgacagt gatgatataa
     2521 acctttattc actggataca gtgaggggaa atattattca gtcatatacc ctggaaggta
     2581 cagttaacag ggactgggaa gaaatatccc aggacaggga tttcatttat ataggggatt
     2641 tcggcaataa ttcatccggg aaccggacag atctgatgat actgagaatc agtaaaaatt
     2701 ctattgttaa tgagcgcctg ataattgaca caatcagatt ctcctatccc gatcagacag
     2761 atttcagtcc tgcttcttca aactacactg atttcgattg tgaggctttt atcgtatctg
     2821 ccgacagcat atatcttttt accaagcaat ggatcagtca aaaaacgacc tgctattccc
     2881 tgccaaaaat accgggcacc catactgctg tcagaaagaa tacacttgat gtaaggggac
     2941 tgatcaccgg tgcagtatat ctggaatcaa aaaaactgat agcactttgc ggttatactc
     3001 ctcttcttga accttttatt tttctgcttt atgattttaa taatccggac tttttcagcg
     3061 gaaataaaag aaggatcgat atatcactgc cttttcacca gactgaagga atcaccactt
     3121 cggacggatt gaaatactat attacaaatg aatatttcca gttcctgaat atccgtcaaa
     3181 aattacatat agtcagcctg gatccattcc tgcaacatta tcttaagccc ggaaacccat
     3241 ccgtggaccg gcccggcaaa aatgatgatt ataaggttta tctcgtgcgt gaagataatt
     3301 gtctgatagt taaaaaaaag gaaaatgaca gaaatgaaga ttattcactg atcagtcttt
     3361 caggacaaac tgtattaaga gggaaactct atggtaaaga gaatgtaata aatatcagcc
     3421 atttaacttc gggcctgtat ttgttcagaa taggaaagaa ttccattcat ccggtaaaac
     3481 tgataaaaat ctgatggcat aaaccagaat ttcaggctta tgtcacggtt aaaattatag
     3541 ttgatgagaa tttactgcca gtatgaaacc tgaaatccgc atcccgggtt ttaatcaaaa
     3601 aagaacgatc agccagtgat ttccgccaat gaagctgtta tgattttaag agcagatcct
     3661 ctaattcttt ccgcttttaa acattctgaa agggtttttc cggtaaatca gtccggcttt
     3721 gcaatttaaa aaaccaaccc gcgagagtgt agttctgttc tggatcatgt atccggccga
     3781 aaagctgatt tccaggtttg gagtaatagt ccggctgatt cctgcaccgg gattaaggaa
     3841 tattttagaa tcatccagag gagcagaatg atctattaat actcccccgt ccagtattgc
     3901 tgaagctgta aaacttttca gataaaaact gtatttgttt tccaggtaaa gaggcagata
     3961 aaaaccttta tcaagctgaa aataacccag cccgattccg cccataaaac tcctggaaaa
     4021 ctggtaaccg aaaacatttg taagtccgaa atagtatttc gacactgccg gatcacagta
     4081 atatgatccg cttccgccat ttatttctga tatattaaca aatccgggcg ttgcctgata
     4141 tctgaatgaa ggattggtaa cggaagttga ctgctgactg agcagtccgg gagaaaataa
     4201 tccgataagt aacagaaaac caatgaattt cttcataata accgtattac aggatcacaa
     4261 atttataaaa aaagcattac acagagttga aataattctc tgaataatac tttataacgt
     4321 atcagcccgc attttgtgtc ttaaaaaatg aaatccggcc ctgtatttta aaaaa
//
LOCUS       DAOSPX010000280         4370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18111_length_4370_cov_3.571495, whole genome shotgun sequence.
ACCESSION   DAOSPX010000280 DAOSPX010000000
VERSION     DAOSPX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18111_length_4370_cov_3.571495"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..2830
                     /locus_tag="PLZ75_11620"
     CDS             161..2830
                     /locus_tag="PLZ75_11620"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOS73082.1"
                     /translation="MYFIKEKRLLVVLLVMFITVTLFSQEKNIVPDSLIVRPDTLLTD
                     TLYVKKISPEAIEKQVTYRADGYKRNDLIKKTSTLVKNAEVNYDNIRITADSIEFNMD
                     TRQVFATGIRDTSGVITGKPVLVVGSETYNADTLVYNFKTGRALVKVIATQQEDGLLR
                     SRIAKMFDDQTTNIYRSTYSTCEADTPHFYINLPRAKVYPGKKIISGPGNLVLEGIPL
                     PLFLPFGYFPIQTKRVASGILMPKPHYEDGRGYALTDGGYYFALNNYFDLTLKGNIFT
                     NGSWLSTVSSTYNKRYKFSGNFSFTYANNINGHKGLPDYRKQANYSIGWSFNQNPKAR
                     PGSTFSASVNMSSSGFDRNNSYNINDHITTQRQSSISYSKTWTGTPFNFSASMNHSQN
                     IRGKHPTVNLNLPKMNFNMSRIYPLKSRRQAGPGKWYQELSFSYSARADNQISTYDSL
                     LFTNKVWDNMRSGFSHEIPVSFQLRPFRNFSITPSLSYSGVLYTQKINKTWDINYRDP
                     ETGQIRPVMVKDTVRGYFYGHSINPSIGAGYSPQIFGLFQFTNPDSRIRAVRHVMKPS
                     VSFSYIPFLKGLSTDMYRQVQSDTTGRMEEYSIFEGGLYGTPSLARKSGNIAFSLTNI
                     LEAKVFERNDTSGKPKNVKLIENFGISTSYNVFADSLNWSPVNMVMRTTLFQNISISA
                     GGNFSFYALDSRGRQTGRFYYTDTKKPLRLTSLGMSLDFSLSDLLKGDESKRRSASTS
                     PAGSRMAQADSRIPAGGPAADSRGRSEAASLFDEYGYMEFDIPWSMNVAYSLNYSKLF
                     SSSQLTQTLRLNGNLSLTKKMNVTFNSGYDFTRKEITMTQIAVTRDLHCWDMSFSWIP
                     NGYVKMWEFSIRVKAAVLSDLKYERRKDYHDNF"
     gene            3075..3452
                     /locus_tag="PLZ75_11625"
     CDS             3075..3452
                     /locus_tag="PLZ75_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOS73083.1"
                     /translation="MKRIIRTAEAPAALGPYSQAVEKKGTLYVSGQIGIDPSTGKLVE
                     GGIQEQTKQIFKNIDAILKAAGYSPQDVVKSTCFLDDISDFAAMNEIYGSYYTEDQPA
                     RSAFAVKDLPAGALVEIETIAVK"
     gene            complement(3559..4005)
                     /gene="rplI"
                     /locus_tag="PLZ75_11630"
     CDS             complement(3559..4005)
                     /gene="rplI"
                     /locus_tag="PLZ75_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOS73084.1"
                     /translation="MEIILLQDIAKLGQKDDLVNVKDGYGRNYLIPKGYAIAATPSAK
                     KMHAENLKQRAHKEEKIKAEAEELATRMEGLKLVIGAKTSSSGKIFGSVNTIQIAEAL
                     REKGFEIDRKSITIPEDQIKEIGNYSAVVRLHREVKINIEFEIVAE"
     gene            complement(4018..4287)
                     /gene="rpsR"
                     /locus_tag="PLZ75_11635"
     CDS             complement(4018..4287)
                     /gene="rpsR"
                     /locus_tag="PLZ75_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOS73085.1"
                     /translation="MAKSESEIRYLTPLTVEIKKKKYCRFKKNRIKYIDYKDPEFLKK
                     FLNDQGKILPRRLTGTSLKYQRKVAQAVKRARHIALLPYVTDLLK"
BASE COUNT         1279 a          979 c          951 g         1161 t
ORIGIN      
        1 atttaaaatg agaaacagca gtaaaacggt attacatata taaaaggcat gctgtttgcc
       61 gtttttataa acagggcaca tttttagttc gcgttttttt aatagtttta ctgccgacat
      121 aatgacgtca cataaaaaca gtacaaaaat aacatttaag ttgtatttta ttaaagaaaa
      181 gcggttactt gtagtgttac ttgtgatgtt catcactgtc actcttttct cacaggaaaa
      241 aaatatcgtc cctgattccc tcattgtcag gccagacacc ctcctgactg atacattgta
      301 cgtcaaaaaa atttcacctg aagctattga gaaacaggta acctacaggg ctgatggtta
      361 taaaaggaat gatctgataa aaaaaacatc caccctggta aagaatgcag aagtcaatta
      421 tgacaatatc agaatcacag ctgattcgat agagttcaat atggatacac ggcaggtttt
      481 tgcaacaggt atcagggaca cgtcgggggt gataaccgga aagccggtac tggtagtcgg
      541 atcggagacc tataatgccg acacgcttgt gtacaatttt aaaacaggcc gggccctggt
      601 gaaggtgata gccacacagc aggaagacgg gctcttgcga agcagaatag caaaaatgtt
      661 tgatgaccag acaacaaaca tttaccgcag cacttactcc acctgtgaag ctgacactcc
      721 ccacttttat attaatctcc ccagagcaaa ggtatatccc gggaaaaaaa ttatatccgg
      781 tccgggaaat ctggtactgg agggaatacc cctgcctctt ttccttccct tcgggtattt
      841 tcccatacag accaagcggg tcgcatcggg aatactgatg ccaaagccgc attatgagga
      901 cggaagaggt tatgccctga cggatggcgg atattatttt gcgctcaaca actatttcga
      961 ccttacctta aaggggaata ttttcacaaa cggttcctgg ctctccacgg tatcatctac
     1021 ctataataaa aggtataaat tcagcggtaa tttttcattc acctacgcga acaacataaa
     1081 tggccacaag ggcctgccgg attacaggaa gcaggcaaac tacagcattg gatggagttt
     1141 caaccagaat ccaaaggcaa ggcccggctc aactttctca gccagcgtaa acatgagttc
     1201 cagcggattt gacagaaaca acagctacaa tatcaatgat catataacga cccagcggca
     1261 gtcaagcatc agctattcga aaacctggac gggtaccccc ttcaattttt ctgccagcat
     1321 gaaccacagt cagaatatcc ggggcaaaca tcccaccgtt aacctgaacc ttcccaagat
     1381 gaatttcaac atgtccagga tttatcccct caaaagcaga cggcaggcag gtcccgggaa
     1441 atggtaccag gaactgtcgt tttcatattc tgcaagggct gacaatcaga tatccacata
     1501 cgacagcctg ttattcacca acaaggtatg ggataatatg agaagcgggt tttcacacga
     1561 aataccggta agcttccagc tgaggccttt caggaatttc tccataacac cctccctgtc
     1621 ctacagcggt gtgctttata cgcagaaaat caacaagacc tgggatataa actacaggga
     1681 tcctgaaacc ggtcagatcc ggccggttat ggtgaaggac actgtgcgcg gatattttta
     1741 cggtcattca ataaatccgt ccatcggcgc aggatacagt ccgcagatct tcggactgtt
     1801 ccagttcacc aatccggatt caaggataag ggctgtcagg catgtgatga agccatcggt
     1861 aagtttcagc tacataccct ttctgaaagg cctgagcacc gacatgtaca ggcaggttca
     1921 gtcagacacc accggaagaa tggaagagta ttcaatattt gaaggcggac tatacgggac
     1981 accttccctt gccaggaaaa gtggaaatat tgctttcagt cttaccaata tacttgaggc
     2041 aaaggtattt gaacgcaacg atacaagcgg aaaacccaaa aatgtcaagc tgattgagaa
     2101 tttcggtatc tccacttcct ataatgtatt tgctgattca ttgaactgga gccctgtcaa
     2161 tatggtaatg aggacgacgc tctttcagaa catcagtatt tcagcgggtg gtaatttttc
     2221 tttttatgca cttgacagca ggggcaggca gacgggaaga ttctattata cggataccaa
     2281 aaaaccgctt cgccttacca gtctggggat gagcctggat ttcagcctga gtgacctgtt
     2341 aaaaggggat gaatcgaaac gcaggtcagc cagcacttcg cctgcaggaa gccggatggc
     2401 acaggcagac tcccggattc cggcaggagg ccctgcggct gacagcaggg gaaggtcgga
     2461 agctgcttcc ctgtttgacg aatacggata catggaattc gacattccct ggtcaatgaa
     2521 tgtggcctac agccttaact attcaaagct tttcagttca tcacagctca cccagacgct
     2581 gagattgaac ggaaacctct cacttacaaa aaagatgaat gttacattca acagcggcta
     2641 cgattttaca aggaaagaga tcacaatgac ccagattgcc gtaaccaggg atcttcattg
     2701 ctgggacatg tcattcagct ggataccaaa cggctatgtg aaaatgtggg agttctccat
     2761 aagggtaaag gcagctgtcc tttccgatct taaatacgag aggagaaagg attatcatga
     2821 taatttttaa atgcaggcgg cattaatgaa taccgggata tcagcagaag aaaacacggg
     2881 cccgggaaac agaatatgaa aagcagaatg aaacaggccg aaccggcaat acgcaaagcg
     2941 gaaaaaatgg acctgaaatg cagaatatga aaagcataat gaaaaaggcc atattcgcaa
     3001 tactcaaagc agaaaatacg gaaacctgaa acaggaatca gaataattga atatcagata
     3061 aacagtcagc aacaatgaaa agaatcatca gaacagcgga agctccggcg gctctcggac
     3121 catacagcca ggccgttgaa aagaagggta ctctttatgt ttcgggtcag atcggtattg
     3181 acccctccac cggaaagctt gttgaagggg gcatacagga acagacaaaa cagattttca
     3241 aaaatattga tgcaatactg aaggctgcag gttacagtcc gcaggatgtt gttaaatcaa
     3301 cctgtttcct ggatgatatt tctgattttg cagccatgaa tgaaatatac ggcagctatt
     3361 atactgagga tcagcctgcc cgttctgctt ttgccgttaa ggatcttcct gcaggtgcac
     3421 ttgtcgagat tgagacgata gctgtaaaat gatcagcctg cccgttctgc ttttgccggg
     3481 attgagacca tagccgtaaa gagattatcc ttaccggcag cctctttcat tttgcgggcc
     3541 ctctctgatc agggaagctt attccgcaac aatttcgaat tcaatattta ttttaacctc
     3601 cctgtggagc ctgacgacgg cgctgtaatt acctatctcc ttgatctggt cctcgggaat
     3661 cgtgatggac ttgcggtcaa tttcaaatcc tttttccctg agggcttcgg caatctgaat
     3721 ggtattcacg gaaccgaata tctttcctga agaacttgtt tttgcaccaa taaccagctt
     3781 caggccttcc attcttgttg caagttcttc agcttcagct ttgatttttt cttccttatg
     3841 agctctttgc ttaagatttt cggcatgcat cttctttgcc gagggagtgg cagcaatggc
     3901 atatcctttg ggaatcaaat aatttcttcc gtagccatct tttacattta ccaggtcatc
     3961 cttctgaccc agtttcgcaa tatcctgcaa taaaatgatt tccattcctt ttcctcctta
     4021 ttttaacaga tcggtcacat aggggagcaa agcaatatgg cgtgcccttt ttacagcctg
     4081 tgcaactttt ctctggtatt taagagatgt gccggttaat cttctgggaa gtattttccc
     4141 ctggtcgttg agaaactttt tgaggaattc aggatccttg taatcaatat actttatccg
     4201 gttctttttg aaacggcaat atttcttctt cttgatctct acagtgagag gggttaaata
     4261 cctgatttct gattcacttt tagccatgcc ttattcctcc atgattttag ctgattcttt
     4321 ttgttttctc tttttctatg cgtattccac agcaaatttg tccattttaa
//
LOCUS       DAOSPX010000281         4343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18257_length_4343_cov_3.809701, whole genome shotgun sequence.
ACCESSION   DAOSPX010000281 DAOSPX010000000
VERSION     DAOSPX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18257_length_4343_cov_3.809701"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PLZ75_11640"
     CDS             complement(<1..953)
                     /locus_tag="PLZ75_11640"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS73086.1"
                     /translation="MLKADKREVVVIGGGAAGMEAAGQLAKAGCIVTLLERESVTGGH
                     VKNWYHLFPDSRNSDEVIEYLARQVNHDNITVRTGVTAGSLEKNGKSIIINTSRGERI
                     RSDAVIVATGFDLFESRRKEEYGYGIYDNVISSADLEMMFRKDSIKRHDGETPRRIGF
                     VHCVGSRDAKVNNLYCSKLCCVTAVKQAMEVKKHIGNASVYCFYMDMRMGGALYEELY
                     KESQEKYGIIYIRGKVSEVSENIDTRLIVKVEDTLAGRPLRMELDMLVLMAGMEMSEG
                     GMGLAESSGLKTGENRFFAPADHHFGTNKSTVAGIFYAGACT"
     gene            complement(946..2007)
                     /locus_tag="PLZ75_11645"
     CDS             complement(946..2007)
                     /locus_tag="PLZ75_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOS73087.1"
                     /translation="MKLEGKKRIWSEYQKEIADDHYFYVRSCIRQSFFPGSEQFFLHF
                     LGDVLGKDIYENPSHTTCTGIGYHSDVVPLSTIMTVIARHYALMKEAGYKHIVISCVT
                     SFGIYTEIFETWKHFPEELEKTREYLYKATGREFDLPESMTHTSDIIYKFRNEIADRA
                     KYRLVNHETGEPLNVVEHIGCHYSKMFPKYGVGGAEFPNVLGGMIEAWGGNVVDYPER
                     RHCCGFGFRQYLVQANRGYSLSNTAKKFSSMKPYKPDLVLTNCPGCTMFMDKWQYTIR
                     EMEGTVYGNNGESIPVLTYEELAGLVMGYNPWDLGLQYHMVQAEPLLKKLGIEYDPAD
                     KFKTKDGRQMPVPQNLINA"
     gene            complement(2026..2739)
                     /locus_tag="PLZ75_11650"
     CDS             complement(2026..2739)
                     /locus_tag="PLZ75_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOS73088.1"
                     /translation="MENYCEMLMEDVRFREGLNACINCGVCTAICPAAEFYDYDPRKI
                     VSLVQTKNNEEIEKLMKSETIWYCGQCMSCKTRCPRGNVPAFVVMALRTLSMKLGFFT
                     ESEKGRQQFAIKRTVGENIFKYGYCVASYAVSPEMHPEQGPIWEYLIQNAPDVYKRLG
                     GGHYDELGAGPLRKIPEDAKEELIKIFEVSGGTELFDDIEKHSRKKAADMGLQFDDEG
                     IDNEYFIEVFTANKKDHHA"
     gene            complement(2863..3516)
                     /locus_tag="PLZ75_11655"
     CDS             complement(2863..3516)
                     /locus_tag="PLZ75_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS73089.1"
                     /translation="MLELKNITKTYHKSSSLFTALDNISLKVDRGDYLLVSGPSGAGK
                     STLLYVAGGMIRPDAGEVKYEGEDISGMNNKAKNEYRRKHVGFVFQQFHLMPFLSVLE
                     NIKMACYERSQLEAVDSYMEKCFLTPLRNKKPSELSVGEKQRTAFVRAIITRPGLLLA
                     DEPTGNLDPANSEILMSLIDDFHREGGTVLLVSHNPVASKYAGKQIVLSEGKIVSGE"
     gene            complement(3585..>4343)
                     /locus_tag="PLZ75_11660"
     CDS             complement(3585..>4343)
                     /locus_tag="PLZ75_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOS73090.1"
                     /translation="EIMGTDVVEGTVHVGSLLSRNIPDNTPFTITGRDGSQYSFEVAK
                     RFEEGRGMLDQGVAFNLHDLQNILGMEGKINKIEALGCICHDGRIKNARKQVQEIFSD
                     LEVAEISNIADARENQRLMMNKYGSFIIPFIILTCLLITALLFYQNVTVRNREIGLLK
                     AMGRGNATILFLFLAKAFILGLTGGVAGFFTGTWIAGYFGKEIFLFTYSAIKPVWSLF
                     IVTLIIFPVLWMLASWIPALRATQIDAAKTLSGE"
BASE COUNT         1120 a         1097 c          835 g         1291 t
ORIGIN      
        1 gtacaggctc ccgcatagaa aatcccggct acagtactct tattggttcc gaaatgatga
       61 tcagcaggtg caaaaaacct gttttcaccg gttttgagac ctgaagattc tgcaagtccc
      121 attccgcctt ccgacatttc catccctgcc ataagaacca gcatgtccag ttccatcctc
      181 agaggccgtc cggcaagggt atcctcaacc tttacaataa gacgggtatc tatgttctcc
      241 gatacttccg atacttttcc ccttatatat attattccgt atttctcctg tgattccttg
      301 taaagttcct cgtacaaggc ccctcccatt ctcatatcca tgtaaaagca atagacactt
      361 gcattcccta tatgcttttt tacttccatt gcctgcttta cagccgtcac acagcatagt
      421 ttggagcaat acaggttgtt tacctttgca tcgcgtgatc ccacacaatg cacaaaacct
      481 attctgcgcg gcgtttcacc gtcatgcctt tttattgaat ccttacggaa catcatttcc
      541 agatcagccg agcttatcac attgtcataa atcccatacc cgtattcttc ctttctccgg
      601 ctttcaaaaa gatcaaagcc cgttgccacg atgaccgcat ccgatctgat tctctctccc
      661 cgggatgtat ttatgatgat tgactttccg tttttttcca gtgaacctgc agttactcct
      721 gtacggacag taatattatc gtgatttacc tgtctggcaa ggtattcaat aacttcatca
      781 ctgttccggg aatcgggaaa aagatgatac cagttcttta catgcccgcc cgttacggac
      841 tccctttcaa gaagcgtgac aatacaaccg gcttttgcaa gttgccccgc agcctccatt
      901 ccggcagccc cgcctccgat aacaacaact tcccttttat cagccttaag cattgatcag
      961 attctgagga accggcatct gtctgccgtc tttcgttttg aacttgtccg cgggatcata
     1021 ttcaattcca agttttttca gcaaaggctc cgcctggacc atgtgatact gcagaccgag
     1081 atcccaggga ttgtagccca taaccaggcc ggccagttct tcataggtca gaaccggtat
     1141 tgattccccg ttgttcccat aaacagtgcc ttccatttcc ctgatggtat attgccattt
     1201 atccatgaac atcgtgcatc ccggacaatt ggtgagaacc aggtcgggct tatagggctt
     1261 catggaactg aatttcttgg cggtgtttga aagggaataa cccctgttgg cctgaaccag
     1321 gtactgcctg aaaccaaatc cgcagcaatg tctcctttcc ggataatcaa caacatttcc
     1381 gccccaggct tcaatcattc ctcccagcac attgggaaat tcagccccgc ctacaccata
     1441 cttcggaaac atttttgaat agtgacagcc gatgtgttca acaacattga gaggttcacc
     1501 agtttcatgg ttaacaagcc tgtattttgc cctgtcggca atctcattgc ggaatttata
     1561 aattatatca cttgtatggg tcatactttc cggaagatca aattctcttc ccgttgcttt
     1621 ataaagatat tcacgggtct tttccagttc ttcggggaaa tgcttccagg tttcaaatat
     1681 ctcggtgtaa ataccgaagg atgtaacaca ggaaattact atatgcttgt atcctgcttc
     1741 cttcataagt gcataatgcc ttgcaatgac cgtcattatt gttgaaaggg gaacaacatc
     1801 ggaatgatat ccgataccgg tacatgtggt gtgggaagga ttctcatata tatcctttcc
     1861 gaggacatcc cccaggaaat gcaggaaaaa ctgctctgac cccggaaaaa agctttgtct
     1921 tatacatgat cttacataaa aataatgatc atcagctatc tctttctgat attcggacca
     1981 gattcttttt ttaccttcta atttcatttt accgggttta tatggttaag catgatggtc
     2041 ttttttattt gcggtaaaca cctcaataaa atattcatta tcaatacctt cgtcatcaaa
     2101 ctgaagcccc atgtcagcag ctttcttcct ggaatgcttt tcaatgtcat caaagagctc
     2161 agttcctccg gatacttcaa aaatttttat cagttcctcc tttgcatctt cgggaatttt
     2221 cctcagcggc ccggctccca gctcatcata atggccccct cccagacgtt tgtaaacatc
     2281 aggagcattt tgaatcaggt attcccagat tggtccctgt tcggggtgca tttccgggct
     2341 gacggcatag cttgccacgc agtatccata cttgaagata ttttctccaa cggtcctttt
     2401 tatggcaaac tgctgacggc ctttttcaga ttcggtaaaa aacccgagct tcatggacaa
     2461 tgtcctcagt gccattacaa caaaagccgg gacattgcct ctggggcacc tggtcttgca
     2521 ggacatgcac tgtccgcaat accagatggt ttcagacttc atgagttttt caatctcttc
     2581 attgtttttc gtctggacca gactgacgat ctttctggga tcatagtcat agaattcagc
     2641 agccggacag atggccgtac aaactccgca attgatacag gcgttgagtc cctctctgaa
     2701 cctcacatcc tccatgagca tttcacagta gttttccatc acagtttcct ttgttgaaat
     2761 acttaaaaca aaagtattgc tatgcctgta aaaaggtaag aagattaaag cctattttaa
     2821 gtggtattta tacgtataag aaggaggaaa gaaatataag gatcattctc cggaaacgat
     2881 tttcccctca ctgaggacta tctgtttccc ggcatatttt gaagcaacgg ggttgtgtga
     2941 gacaagcagg acagtacctc cttccctgtg gaaatcatct atcagtgaca tcagtatttc
     3001 actgttcgcc ggatcaagat tgcccgtggg ttcatccgcc agcaaaagac cgggccgggt
     3061 aatgattgcc cttacgaaag cggttctttg tttttctccg acactgagct ctgaaggctt
     3121 cttattcctt aatggtgtaa gaaagcattt ttccatgtag ctgtcaacag cctccagctg
     3181 agacctttca tagcatgcca tttttatatt ttccagcacc gacagaaagg gcatcagatg
     3241 aaactgctga aacacaaagc ccacatgttt cctcctgtat tcattttttg ccttgttgtt
     3301 catgccggaa atatcttctc cctcatattt cacttctccg gcatccggtc gtatcatacc
     3361 ccccgcaaca tacaacaggg ttgattttcc cgcaccactc ggtccgctga ccaggagata
     3421 atcccccctg tcaactttga ggctgatatt atcaagagca gtaaaaagtg aagagctttt
     3481 atgatatgtt ttcgtaatat ttttaagttc aagcataaat atgaaattta tattgtttta
     3541 caactgcttc cgggccggat acggggaaaa ggaaagagaa acattcattc cccgctcagg
     3601 gtttttgcgg catcaatctg ggtagccctc agtgcgggta tccagcttgc cagcatccag
     3661 aggacgggga aaatgatcag ggtgacgatg aacaggctcc agaccggttt aatggcactg
     3721 tatgtaaaaa gaaaaatctc tttcccgaaa tatccggcta tccatgtgcc tgtgaaaaat
     3781 ccggccaccc ctcctgtcag tccaagtata aatgcctttg caagaaacag aaacaatata
     3841 gttgcatttc ctcttcccat agccttaagc aatcctattt cacggttcct tacggttacg
     3901 ttctggtaaa aaagaagagc ggttattaac aggcatgtca gaatgatgaa gggaattatg
     3961 aatgatccgt atttattcat catcagtcgc tgattctccc ttgcatcggc aatattgctt
     4021 atttctgcaa cttccagatc tgaaaatatc tcctgtacct gtttccgggc attttttatc
     4081 ctgccgtcgt gacaaatgca tccaagggct tcgatcttgt ttattttgcc ttccataccc
     4141 agaatattct gaagatcatg aaggttaaaa gccacaccct gatccagcat ccccctgcct
     4201 tcttcaaacc ttttagccac ctcgaaactg tactgactgc catccctgcc cgtaatggta
     4261 aagggtgtat tgtccggtat attccgcgaa agaagagaac cgacatgaac tgttccttcc
     4321 acaacatcgg ttcccatgat ttc
//
LOCUS       DAOSPX010000282         4323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18368_length_4323_cov_4.193299, whole genome shotgun sequence.
ACCESSION   DAOSPX010000282 DAOSPX010000000
VERSION     DAOSPX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18368_length_4323_cov_4.193299"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..587
                     /locus_tag="PLZ75_11665"
     CDS             264..587
                     /locus_tag="PLZ75_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73091.1"
                     /translation="MKTLKLLTLFFFLIGFINCEKNKSGKENSYVFIKPYCDMVTVGG
                     VVGIREKCFNSGDTVSGRVITDGQVTIRIAAHSELNEGPPGPHSYQEFLNVPAEYLKK
                     IKDRN"
     gene            complement(599..1252)
                     /locus_tag="PLZ75_11670"
     CDS             complement(599..1252)
                     /locus_tag="PLZ75_11670"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOS73092.1"
                     /translation="MNASVRERPENIETNENTDPGRTRANELISRIAGILISLTALCV
                     LSPLIIILSFLIKVSGKGPVIYSQDRIGKNGKPFVIYKFRSMVFNAETGLPRLSCSNE
                     ERITRIGKFLRKYRIDEIPNFINVIKGDMSIVGPRPERKFFIDQIMERNPAFSELLRI
                     KPGITSWGQVKYGYASDVDQMLERLEYDLYYIRNKSLFLDIKILFCTTGTILKGKGL"
     gene            1331..1978
                     /locus_tag="PLZ75_11675"
     CDS             1331..1978
                     /locus_tag="PLZ75_11675"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOS73093.1"
                     /translation="MTDDFEARGKRKKLISELRKKGIGDEEVLRAMDAVPRHVFMDNA
                     FLNHAYVDKAFPISSGQTISQPYTVAVQTSLLEVKRRSRILEVGTGSGYQAAILAEMG
                     AKVYTIERYRELFLKAQKTLASLGYSVDSFFGDGYAGKPQYGPYDGIIITAAIKEVPE
                     ELLKQLKTGGRLVAPVGDSSSQRMTLVRRTGEDSYEYSSHGRYLFVPMLPGIVKE"
     gene            1988..2623
                     /locus_tag="PLZ75_11680"
     CDS             1988..2623
                     /locus_tag="PLZ75_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS73094.1"
                     /translation="MEIYRLVFSPIDVNTYILADSDNICAVIDCGCYSDEEFKILTDF
                     IVDKNLSPQLLLNTHCHLDHIFGNGRFLEKYGLGTFCHTGEENNRKGAVMQAKFFGLS
                     MEMPPEPAGFLEDGQIVSFGSVSLKALHVPGHSPGSLAFYCEREGVLFTGDALFAGSI
                     GRSDLPGGDHNTLIKSITGKLFTLPEETIIYPGHYESSTIGEEIKTNPYFA"
     gene            2963..>4323
                     /locus_tag="PLZ75_11685"
     CDS             2963..>4323
                     /locus_tag="PLZ75_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HOS73095.1"
                     /translation="MSHEMFANRHNGPRDHEIPSMLKTVGVESLNELIDKTVPAGIRL
                     SGPLDLPPAMTEYEYLAHLRKLGSKNKQYRSFIGQGYYGVAPLSVIIRNVLENPSWYT
                     SYTPYQAEISQGRLEALINFQTMIIELTGMEIANASLLDEATAAAEAMIMMFNARPRE
                     AVKAGINRFFADEDVFPQTLDVLRTRAMPLGIEVVTGKYSDIVPDKSFFGYFVQYPSG
                     SGEVKDYSDFAAKVHEAGALLSVAADLMSLALLVPPGEWGADIVAGSSQRMGLPVSFG
                     GPHAGYFATRDALKRSMPGRIIGVSVDVHGNTALRMALQTREQHIKRERATSNICTAQ
                     ALLASMSGMYAQYHGPEGLKSIASHIHRSACTLNDSLKELGYRQLNKVFFDTLKIELP
                     ENISRDKIRDLALARAYNFHYPSDGSVIISTDEITSLEEINVISAIFAEAAGKKTPLT
                     EKL"
BASE COUNT         1212 a          888 c         1068 g         1155 t
ORIGIN      
        1 caggagagga agaatacagc acgacattcg tggttgaata acactaaaga ggttttatgc
       61 cgtagagttt attataaaat ttaaccggta aaccatgtaa ttcatacaaa aaggcttaat
      121 aatgttgcag cgttttaacc ggtaaaagcg tatatataaa aaagagcagg gaagtcagta
      181 tcatgctgta attgaaaagc cgtccggaca aaaaaatatc cgcgttgtgg aaattgtcaa
      241 cctttcttta acacaatatt attatgaaaa cacttaaact tcttacactc tttttttttc
      301 ttatcggatt tatcaactgt gaaaagaata aatccggaaa agagaacagc tatgtcttca
      361 taaaaccata ctgtgatatg gtgactgttg gcggagttgt cggaatcaga gaaaagtgct
      421 ttaattcggg tgatacggtt tctggcagag taataactga tgggcaggtt acaatccgga
      481 ttgctgctca ctcagagctt aatgaaggtc ctccgggccc tcatagttat caggaattct
      541 taaacgtgcc tgctgaatat ctgaagaaaa taaaggaccg gaattgaaaa tctgattctt
      601 aaagtccctt gcctttgaga atagtaccgg ttgtgcagaa taatatcttt atatcaagga
      661 acagcgactt attacggatg taatagaggt catattcaag tctttccagc atctgatcaa
      721 catccgaggc ataaccgtat ttaacctgtc cccatgaagt gataccgggc tttattctga
      781 gaagttccga aaatgcagga ttcctttcca tgatctggtc aatgaaaaat tttctttccg
      841 gccggggccc gactatcgac atgtctccct ttataacatt tatgaaattt gggatttcgt
      901 caatccggta ttttctcagg aattttccga tacgggtaat cctttcctca tttgaacatg
      961 agagtcttgg aagacccgtt tctgcgttga agaccatgga acggaactta taaatgacaa
     1021 agggtttgcc gttttttcct atcctgtcct gggaatatat aacgggacct ttgcctgata
     1081 cttttatcag gaatgaaagg attattatca gtggactcaa tacacataat gcagtcaggg
     1141 atatcaggat gccggcgatc cgactgatca gttcattcgc tcttgtcctg ccagggtcag
     1201 tattttcgtt tgtttctata ttttcaggtc tttcccgtac ggatgcattc acatgttccg
     1261 gacatttatt gccggcgaag atagaaattt ttttattttt gctcaatgta ccctattcac
     1321 acgagaataa atgacagatg attttgaagc caggggaaag agaaagaagc ttatctcgga
     1381 actcaggaag aaaggtattg gtgacgagga ggttctgagg gccatggatg ccgttccccg
     1441 tcatgttttc atggataatg cctttctgaa tcatgcctat gtggacaaag cttttccgat
     1501 cagctcggga cagaccatat cccagcccta taccgttgcc gtacagacat cattactgga
     1561 agtgaaaaga aggagcagga tactcgaggt tggtaccggt tcaggttacc aggctgcaat
     1621 actggctgag atgggagcaa aggtttatac tattgaaagg tacagggaat tatttctgaa
     1681 ggcacagaaa acccttgctt ccctgggcta ttccgttgat tccttttttg gcgatggtta
     1741 tgcgggaaag cctcaatacg gaccctatga cggaataata atcactgcag ccataaagga
     1801 agtgccggaa gaacttctga agcagctgaa gacggggggc aggctggtgg ctcctgtggg
     1861 tgacagttct tcacagcgta tgacactggt aaggcggaca ggggaagatt cctatgaata
     1921 ctcctcacat ggccgctacc tttttgttcc catgctgccc ggtatagtaa aagaataatt
     1981 gtaggaaatg gaaatataca gacttgtttt ttcacccatc gatgtaaata catatattct
     2041 tgcagacagt gataatatat gtgccgttat cgactgcgga tgctacagcg atgaagaatt
     2101 caagatcctg actgatttca ttgtggataa gaatcttagt ccgcagctgc tgctgaacac
     2161 tcactgccac cttgatcata tattcggaaa cggacgcttc ctggagaaat acggactggg
     2221 cacattttgt cacacgggag aggagaataa ccgtaagggt gccgttatgc aggcaaagtt
     2281 tttcggcctg agcatggaaa tgccgccgga acccgcggga ttcctggaag acggacagat
     2341 tgtatcattc ggatcggtaa gcctgaaagc cctgcacgtt cccggccatt ccccgggcag
     2401 ccttgctttc tactgtgaaa gggaaggagt gttgtttacc ggggatgcgc tctttgccgg
     2461 aagcattggc agatctgatc ttcccggagg cgatcacaac acacttatta aaagtattac
     2521 cgggaaatta ttcaccctgc cggaagaaac aataatatac ccggggcatt atgaatccag
     2581 tacaataggg gaggagataa aaacaaatcc ttattttgcg taactttcag atgttatttg
     2641 aaataatcat gagtcagact gaaactttta catgtataaa ggtgttgatg tttcagatct
     2701 gtgcctcagc tcctgaataa agctttgaac aaaggcagga tgaatcaggt tatatgagga
     2761 tgtttccgga aatggaggaa gatttctccg ggttggttgt ggaaataccg gatttgtctg
     2821 agggaattgc ggatttgccg gagaaaattt cagggtggat ccgggcatgt ttccggatga
     2881 ctgcggaagc gcttcatgaa aggctttgca aggcagattg aatagcataa aaagaaaata
     2941 atataaacag aaaacttaaa tcatgtccca cgaaatgttt gctaaccgtc ataatggtcc
     3001 gcgtgatcat gagattccgt caatgctgaa aaccgttggt gttgaatcgc tgaatgagct
     3061 tattgacaag actgttcctg cagggataag actttccggg cctcttgacc ttccgcctgc
     3121 catgacagaa tatgaatatc tggcccatct ccggaagctc ggatcgaaaa acaaacagta
     3181 ccggtccttc atagggcagg gttattacgg ggtggctccg ctttcggtca taataaggaa
     3241 tgttcttgag aatccctcat ggtatacttc ctatacaccc taccaggctg aaatatccca
     3301 gggaagactt gaggccctga tcaattttca gaccatgatc attgaactga ccggaatgga
     3361 aatagccaat gcctcactgc tggatgaggc aaccgcggcg gccgaggcga tgataatgat
     3421 gttcaatgca aggccgcgtg aagctgtaaa ggcagggatt aacaggttct ttgcagatga
     3481 ggatgtcttt ccccagaccc tggatgtttt aagaacacgt gcaatgcctc tcgggattga
     3541 agtggtgaca gggaaataca gtgacattgt accggataaa agctttttcg gatatttcgt
     3601 gcagtatccc tccggatcgg gggaagtaaa ggattacagt gattttgccg caaaggttca
     3661 cgaagcaggt gccctgctga gtgttgctgc tgacctgatg agcctggccc ttcttgttcc
     3721 tccgggggaa tggggcgccg atattgttgc agggtccagt cagcggatgg gattgccggt
     3781 aagtttcggg ggaccacatg ccggttattt tgccaccagg gatgccctga aaaggagcat
     3841 gcccgggagg atcataggtg tttcggtaga tgtacacggg aataccgcac tcaggatggc
     3901 gctgcagacc cgtgaacagc acataaagcg ggaaagggca acgtcgaata tctgtacggc
     3961 ccaggcactg ctggcatcca tgtcgggtat gtatgcacag tatcatggcc cggaaggact
     4021 gaaaagtatt gcatcgcata tccacaggtc ggcctgtacc ctgaacgatt ccctgaagga
     4081 acttggctac aggcaactca acaaggtgtt ctttgataca ttgaagattg aactgccgga
     4141 aaatatttcc cgggataaaa tcagggatct tgccctggcc agggcatata atttccatta
     4201 tccttcagat ggttcggtta taatcagtac ggatgagatc accagccttg aagagataaa
     4261 tgtcatctct gccatattcg cggaggctgc cgggaaaaag actccactga ccgaaaaact
     4321 gtg
//
LOCUS       DAOSPX010000283         4317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18406_length_4317_cov_5.096434, whole genome shotgun sequence.
ACCESSION   DAOSPX010000283 DAOSPX010000000
VERSION     DAOSPX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18406_length_4317_cov_5.096434"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PLZ75_11690"
     CDS             <1..731
                     /locus_tag="PLZ75_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS73096.1"
                     /translation="HKKILVTDSENRTYPMAYSIAEDLDGNIWIGTDQGPLVYYSPER
                     IFKNDLKAYRIRIPRSDGSGLADYLLGTETVTAIAVDGSNRKWLGTAGSGVYLVSADG
                     TTEILNFNESNSPLLSNSILSISIDGKSGDVWFATSKGIQSFRGDATSGSENFTDVFA
                     FPNPVREDFQGNLTISGLMRDTRVSITDISGNLVYRTVSDGGTASWDLRTYNGKRVAT
                     GVYLVFCASDDGSRSCVIKVLVIN"
     gene            complement(875..2176)
                     /locus_tag="PLZ75_11695"
     CDS             complement(875..2176)
                     /locus_tag="PLZ75_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOS73097.1"
                     /translation="MVTKNNEELVKRIARLVRQNKELEEQLKVLQKKIGRLTEDNEQL
                     KADREKLISSGTVEGKVSAREERPLKFEMATVLLSDIHTDSILSEGGDSASVMDELDE
                     IIYEFDRIALKYKVEKIKTIGDSFMCAGGIPVKNITNPVDVVMAALEMRTFLSDFESA
                     KRPDGKKLWKFKVGIHTGPVTASVTGKKKISYDIKGDTVNSASRLEKISEKGQILISV
                     MTYELVKEFFECEYFGKLPVKYKGDLQVYRVKGLKEEFSAGGRGTVPNDAFRIKFGLI
                     RFTDIQEIVLDKLERELPGHLYYHNVKHTVDVVTEVELIGLAEGCSDEEILILKTAAL
                     FHDTGHTVDYDEHEFHGTLIAREMLPDYDYTPGEIDLVCSLIMSTRLPPKPSSLLEEI
                     ICDSDLDYLGRSDFIPVSNSLFEELKARNKMGSLMNGTSSR"
     gene            2275..3000
                     /gene="recO"
                     /locus_tag="PLZ75_11700"
     CDS             2275..3000
                     /gene="recO"
                     /locus_tag="PLZ75_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOS73098.1"
                     /translation="MIESTKAIVLHQIRYSDSAAIIRFFTSKFGRMSCMVRGMGNRKA
                     GRNKVLFQPMSILDLSLNYRESREIQTIRDFSPSYSPSDIWSDIRKCTVAIFLGEVLT
                     SVLREESPNEPLFRFIEDSVVYFDKSREGNANFHLAFMAQLTGFLGFEPSGDRHSRSI
                     FFDMLNGTFTDRPPGHRHFANPEISAALSALFSTSPEEADKIALKGSLRNEILDTLMN
                     YFSLHLPGIKKINSLEVLRQVYI"
     gene            3051..>4317
                     /locus_tag="PLZ75_11705"
     CDS             3051..>4317
                     /locus_tag="PLZ75_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOS73099.1"
                     /translation="MMPIISTIFNSVNSSRLKQIDHFKEYPAETQDRVFSRLVETASG
                     TEWGRKYGYSSLASAKEFSERVPLQTYEDLMPYIERIGNGEGDILWPGKIRWFAKSSG
                     TTSSKSKFIPMSREALYETQFRAVKDLLAIYIRNNPETRIFLGKSLTLGGSHRIGQFG
                     SRALCGDLSAVMIENTPLYAEIVRTPKKKIALTDDFEKKLELITKATINRNVTSISGT
                     PSWYLSLIKYILAYTGKSNLLEVWPNLEVFFHGGINFNPYRETYRQLIPGSQMNYMET
                     YNASEGFFAIQEDPASDELLLMLDYGIYYEFVPADRYKHPDPPAYSIAEVEKGRNYVM
                     VISTSGGLWRYIMGDTVVFTGLNPHRIRITGRTKYFINAFGEEVIKENAEEALRKACL
                     STGAVISEYTAGPVYMGASSKGSHEWIIEF"
BASE COUNT         1157 a         1054 c          955 g         1151 t
ORIGIN      
        1 ttcacaaaaa gatcctggta acagattcgg aaaaccggac ttaccccatg gcttactcaa
       61 ttgccgagga tcttgacggc aatatctgga tcggaacaga tcagggcccc ctggtctatt
      121 acagtccgga aaggattttc aagaatgacc tgaaagccta ccgtatcagg attccccgca
      181 gtgacggttc cggtctggcg gattatctgc tcggtaccga aacagttacc gccatagctg
      241 ttgacggatc caacagaaaa tggctcggga ccgcgggttc cggagtatat ctggtttctg
      301 ccgacggaac aacggaaata ttaaacttca atgaatcaaa cagccccctg ctttcaaact
      361 caatcctgag catcagtata gacggcaaat cgggtgatgt ctggtttgcc acctcaaagg
      421 gcattcaatc cttccgcggc gatgcaactt caggatctga gaactttacg gatgtttttg
      481 cctttcccaa tccggtaagg gaagattttc aggggaatct tacaatttcc ggactgatga
      541 gggatacccg ggtcagcata accgacataa gcggaaacct tgtgtacaga actgtttccg
      601 acgggggaac agcttcatgg gatctcagaa cctataacgg caaacgtgtg gcaacaggcg
      661 tgtacctggt cttttgtgca tccgatgacg gaagccgttc atgcgtgatc aaggttctgg
      721 tgattaactg atatccggaa gctgaaaaag ttcccgtgat taaacgatat ccgtcagctg
      781 ataaggcttt gtatcctctt tatctgctgc tgcttattta cttccctgag ccttctggcc
      841 gtcctggtga aatactggtg ccccgaaata aattttacct ggagcttgtt ccattcatta
      901 gggatcccat tttgttccgg gccttcagtt cttcaaaaag agaattggat accggaataa
      961 agtcgctgcg cccgagataa tcaagatccg aatcacatat tatctcttca agcagggagg
     1021 agggcttcgg aggaagcctg gtcgacatta tcagtgaaca gacaagatca atctcaccgg
     1081 gtgtgtaatc ataatccgga agcatttccc tggcgataag tgttccatgg aactcgtgct
     1141 cgtcatagtc caccgtatgt cctgtatcat gaaacagtgc agccgttttc aggatcagga
     1201 tctcctcatc ggaacatcct tccgccagac caatgagctc tacttcagta acaacatcaa
     1261 cagtatgttt tacattgtgg taatagaggt gtccgggaag ctccctttca agcttgtcga
     1321 gaacaatttc ctgaatatcg gtaaaacgga tcagcccgaa ttttatcctg aatgcatcat
     1381 tcggaacagt tcccctgccg ccggccgaaa attcctcttt aagccccttt accctgtata
     1441 cttgcaggtc acccttatac ttaaccggca gcttgccgaa atattcacat tcaaaaaact
     1501 cctttaccag ctcataggtc attaccgata tcagtatctg tcctttttcc gaaatcttct
     1561 caagccggct tgctgaatta accgtatctc ccttgatatc gtaactgatt ttctttttgc
     1621 ctgttacgct ggcagttacg ggacccgtat gaatacccac tttaaacttc cacagctttt
     1681 ttccgtccgg gcgctttgct gattcaaaat ccgacaggaa agttctcatt tcaagagcag
     1741 ccataaccac atcaaccggg ttggttatgt ttttcaccgg aattcctccg gcacacatga
     1801 aactgtcccc tatggtcttt atcttttcaa ccttgtattt cagggctatc cggtcaaact
     1861 catagattat ctcatccagc tcgtccatca ccgaagcgga gtcacctccc tctgacagga
     1921 tggaatcagt atgaatgtcg gacaacagta cggttgccat ttcaaatttc agcggcctct
     1981 cttcccgggc agacaccttc ccctcaactg taccggagga aataagtttt tcccggtcag
     2041 ctttcagctg ttcattatcc tccgtcagtc tgccgatttt tttctgcaga accttcagtt
     2101 gttcttccag ctccttgttc tgcctcacga gccgggcaat cctctttaca agttcctcat
     2161 tgtttttagt gaccatcaga cagcaggttg atgggcaaat atagcaattc cggcatcaca
     2221 attcaatagc agtaatcagc aaattgtttt aattttgaac aaacaaggcc tccgatgatt
     2281 gaatcaacaa aagcaatagt ccttcatcag atcagatatt ccgattcggc tgccattatc
     2341 cgctttttca ccagcaaatt cgggaggatg tcctgcatgg tcaggggaat gggaaacagg
     2401 aaagcaggga ggaacaaggt tctgttccag cccatgtcca tactggatct ttcattaaac
     2461 tacagggaat cgagggaaat acagacaatc agggattttt caccatccta ctccccttct
     2521 gacatatggt ctgatataag gaagtgcact gttgcaattt tcctcggaga ggtgcttacc
     2581 tcggtcctcc gcgaagagag cccgaatgag cccctgttca ggtttattga agattccgtg
     2641 gtgtatttcg acaaaagccg tgagggcaat gccaatttcc acctggcatt catggcgcag
     2701 ctgacgggat tcctgggatt tgaaccttca ggagacaggc acagcagaag catttttttt
     2761 gatatgctta acgggacatt tactgaccgc cctcccggac acaggcattt tgccaatcct
     2821 gaaatatcag cggccctttc ggcacttttt tccacatccc cggaggaggc agataaaatc
     2881 gccctgaaag ggtcgttgag aaatgaaatt cttgacactc tcatgaatta tttctcgctt
     2941 cacctgcccg gaataaaaaa aataaattcc ctggaagtcc tgcgacaagt gtatatctga
     3001 ttcagaattt ttaatgaatt tgactatctt tatttcctga cctttttcca atgatgccga
     3061 ttatatcaac aatattcaat tcggttaatt ccagtcgact gaaacagatc gaccatttca
     3121 aggaatatcc tgccgaaaca caggacaggg ttttttcccg gcttgttgag acggcttcag
     3181 gaactgaatg gggaaggaaa tatggttact catccctggc ttcggcaaaa gagttcagtg
     3241 aaagggttcc gcttcagacc tatgaggatc ttatgccgta tattgaaaga atcggaaacg
     3301 gtgaaggaga tatcctgtgg ccgggaaaaa tcagatggtt cgccaagtcc tcaggaacca
     3361 cctcttcaaa aagcaagttc attcccatga gccgtgaggc tctttatgaa acccagttca
     3421 gggcggtgaa ggatctcctt gccatttata tcaggaataa tcccgaaacc cgtatcttcc
     3481 tgggaaaatc acttaccctc ggagggagcc accgtatcgg tcagtttggc agcagggcat
     3541 tatgtggtga cctttctgcc gtcatgattg aaaacactcc cctttatgct gaaatagtca
     3601 gaacaccgaa gaaaaaaata gcccttaccg acgattttga gaaaaagctt gaactcataa
     3661 caaaggcaac catcaacagg aacgtcacaa gtatatcagg cactccctca tggtatctga
     3721 gcctgataaa atatattctc gcctacacgg gaaaatcaaa cctgcttgag gtatggccca
     3781 atcttgaggt tttcttccat ggcggcatca acttcaatcc ctaccgtgaa acttacagac
     3841 agcttattcc cggcagtcag atgaattata tggaaaccta taatgcatcg gaaggatttt
     3901 ttgccattca ggaggatccg gcttcggacg agttgctgct gatgcttgat tacggaatat
     3961 actatgaatt tgtgccggca gacaggtata agcatcccga ccctccggca tacagcattg
     4021 cggaggttga aaagggccgc aactatgtaa tggtcatttc taccagcggc ggattgtgga
     4081 gatacattat gggagacacg gttgtattta ccggcctgaa ccctcaccgc atacgcatca
     4141 cgggcagaac aaaatatttt ataaacgctt tcggcgagga agtcataaag gaaaatgctg
     4201 aagaagcact gagaaaggcc tgtctgtcaa caggtgccgt catctcggaa tacaccgccg
     4261 ggccggttta catgggagca tcatcaaagg gatcgcatga atggatcata gagttcg
//
LOCUS       DAOSPX010000284         4298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18506_length_4298_cov_4.187367, whole genome shotgun sequence.
ACCESSION   DAOSPX010000284 DAOSPX010000000
VERSION     DAOSPX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18506_length_4298_cov_4.187367"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3743
                     /locus_tag="PLZ75_11710"
     CDS             <1..3743
                     /locus_tag="PLZ75_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOS73100.1"
                     /translation="PESPVPVDALPDIGENQQIVSTEWMGRSPRDIQDQVTYPLTTAL
                     LGIPGVQTIRSSSMFGMSFIYIIFNDNVEFYWSRSRILEKLNSLPPGTLPAGVQPSLG
                     PDATALGQVFWYTLEGRDPKTGLPAGGWDPQELRAIQDYYVKYSLSAAEGVAEVASAG
                     GFVKEYQVDLDPDALRAFNVSVRDVMHAVQSSNLDIGAETIELNYAEYLIRGLGYVKS
                     LEDLEYSVVAVRNNIPVRIRDIARVSFGPSPRRGGLDKSGSEAVGGVVVARYRSNPWA
                     VIGNVKDRIAEVEAGLPQKILPDGTVSKVTIVPFYDRTGLIRETIGTLETALSHEILI
                     SIIVILVLLLNLRASLIVSGLLPAGVLMTFILMRLAGIEANIVALSGIAIAIGVMVDI
                     GIVDVENILRHLEMPENRGVKGERLLKVIYEATVEVRGAVVTSVSTTIVSFLPVFAMQ
                     AQEGKLFHPLAFTKTFALLSAFILGIVILPTLVYLFFNITSGRKTTKKAWNFLLIASG
                     LIIAAVWQTWPALALTAAGINNLLAGRWSERRKNYPGHINIAIVVLFALWYLAKGWLP
                     LGPHNSQFVNFLFVAGIVAVILLALMSLVRYYENIMGWALKNKLKFLLIPGATLLFGW
                     LAWMGFDRTFGFIAGSFEKAGWKKIRQTALWQGASGIFPGAGKEFMPALDEGSFLLMP
                     TSMPHTGIEKNLEYIEILDRRVAAIPEVEAAVGKWGRVNSALDPAPVQMFENTINYRP
                     EYILDENGRRMRFRTDRTGSFILKGDLKYNSGSDKFRLIPADSLIPDRKGDFFRQWRP
                     EIKSPDDIWKEIVKATAIPGLTSAPKLQPIETRLVMLSTGMRAPMGLKVYGPDLEAIE
                     HAGLQFEKALKDVPSVNPSTVFYDRAVGAPYIEIRLRREAMARYGITVSELQEVLQVA
                     VGGMSLTTSVEGRERFPLRVRYARELRDNPEDLGKILIPAAGGIQVPLGAVADIDYTK
                     GAQMIQSENTFLVGYVIFDKHEGKAEVDVVNEAAGILRAGIDSGDIELPPGVSYKFAG
                     NYEHQIRATKRLSVVIPLSLIIILFLLYQRFRTITASFIHFSGVFVAFAGGFIMLWLY
                     GQDWFMNFNLAGMNMRELFQMHTINLSVAVWVGFIALFGIATNDGVLMGTYIHQIFEE
                     RQPATVNEVRKAVLDAGKKRVRPAMMTTAVAVIALLPVLSSTGKGSDIMVPMAIPAFG
                     GMLIQVMTIFVVPLFQAIWRETEINKSAAPGPAERIITEGTQ"
     gene            3765..>4298
                     /locus_tag="PLZ75_11715"
     CDS             3765..>4298
                     /locus_tag="PLZ75_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS73101.1"
                     /translation="MQEKEKHITKSGSAGSGNNSGLIHSGRKRALLYIASFYVFLFSL
                     SPLHSQEDSLKKYIEIAVANNPSLLKKDSEYEAFQKKAVQAGSLPDPQLDAGLFLSPM
                     EIVSGRQYADLRLMQMFPLFGVLKNAEDEMRLLARAKREEYIDSELELVYNVRRTWYE
                     LFRLRREIGISEKNLEIL"
BASE COUNT         1066 a          963 c         1185 g         1084 t
ORIGIN      
        1 tgcctgaaag cccggttccc gttgacgctc tacctgatat aggtgagaat cagcagattg
       61 tttctactga atggatggga agatcaccca gggacataca ggaccaggtg acctatccgc
      121 tgaccactgc cctgcttgga atacccggtg ttcagactat ccggagcagt tcaatgttcg
      181 gaatgtcatt tatttacata attttcaatg acaatgtgga gttttactgg agcaggtcac
      241 ggatacttga aaaactcaat tccctgcctc cgggcactct gcctgcaggt gtgcagccat
      301 cccttggtcc ggatgcaacc gcactcggtc aggtattctg gtatacactt gagggccgtg
      361 atccgaaaac cggactgccg gccgggggat gggatcccca ggagctgagg gcaattcagg
      421 actactatgt gaaatattca ctttcagcgg ccgaaggtgt tgcggaagtg gcttcggcag
      481 gagggtttgt aaaagaatac caggttgatc ttgatccgga tgctctaagg gcattcaatg
      541 tttccgtcag ggatgtcatg catgccgttc agagcagcaa tctcgacata ggagcggaaa
      601 caattgaact caattatgcc gaatacctga taagagggct gggttatgtg aaaagccttg
      661 aggatctgga gtattcagtt gttgcagtaa ggaataacat tcccgtccgc atcagggata
      721 ttgcaagggt cagtttcgga ccatctccgc gcaggggagg cctcgacaag tcgggttccg
      781 aagcagttgg cggtgtggta gtagcccgtt acaggtcaaa cccctgggct gtgatcggca
      841 atgtaaagga caggatagcg gaggtggagg ccggtctccc tcagaagata ctccctgacg
      901 gaacagtttc aaaagtgacc atagttcctt tttatgaccg cacaggactt atcagggaaa
      961 ccatcggtac gctggaaaca gctctctccc atgaaatact tataagtatt atcgtaatac
     1021 tggtactgct tctgaacctc agagcctcac tgatagtgtc gggcctcctg ccggccgggg
     1081 tgctcatgac attcatcctg atgagactgg cagggataga ggcaaacatt gttgcattat
     1141 cgggtattgc cattgccatt ggagtgatgg ttgacatagg gatagtggat gtggaaaata
     1201 tactccggca tctggaaatg cctgagaacc gcggggtgaa aggggaaagg ctgctgaaag
     1261 tcatatatga ggctaccgtg gaggtaaggg gtgctgtggt cacttcggtt tccacaacga
     1321 ttgtcagttt ccttccggtt tttgcaatgc aggcacagga aggaaaactg tttcatcctc
     1381 tggcctttac caaaaccttt gcgcttctct cagcatttat tctcggtata gtaatccttc
     1441 ccaccctggt ctatctgttt ttcaatatca catccgggag aaaaaccacc aaaaaggcct
     1501 ggaacttttt actgattgcc tcaggactga tcatagcggc ggtctggcag acatggcccg
     1561 cacttgccct tactgcggca ggtatcaaca acctccttgc aggaaggtgg agtgaaaggc
     1621 ggaagaatta cccgggtcat ataaatatag ccattgtggt actttttgcc ctctggtatc
     1681 tggcaaaagg ctggcttccg ctcgggcctc acaacagcca gtttgtcaat tttctctttg
     1741 ttgcggggat agttgctgtc attctcctgg cactcatgtc gctggtccgt tattatgaaa
     1801 atattatggg atgggccctc aagaacaagc tgaaattcct tttaattccg ggagccactc
     1861 tgcttttcgg atggcttgcc tggatgggtt ttgacagaac cttcggcttt attgccggca
     1921 gctttgaaaa ggccggctgg aagaaaataa ggcagaccgc cctgtggcag ggtgcatccg
     1981 gtattttccc cggagccggc aaggaattca tgccggccct tgatgagggt tcgtttctgc
     2041 ttatgcccac aagcatgcct cacacgggca ttgagaagaa tcttgaatac atagagatcc
     2101 ttgacagacg ggtcgccgcc attcctgaag tggaagcagc cgtcggcaaa tggggaaggg
     2161 ttaactcggc cctcgatccc gctcccgtgc agatgtttga aaatacgatc aattaccggc
     2221 ctgaatatat tctcgacgaa aacgggcgca ggatgcgctt ccgtactgac cgtacaggta
     2281 gtttcatatt gaagggcgac ttgaaatata actccggatc agataaattc aggcttattc
     2341 cggctgacag cctgatacct gacaggaaag gtgatttttt ccggcagtgg aggccggaaa
     2401 taaaatcgcc cgatgatatc tggaaagaga tcgtaaaggc aactgccatt ccgggtctga
     2461 cttcagcgcc gaagctgcag cccattgaga ccaggctggt gatgctgtcc acggggatgc
     2521 gtgctccgat gggactgaag gtttacggtc ccgacctgga ggcgattgag catgccggcc
     2581 tgcagtttga gaaagcactg aaggatgtgc cttcggtcaa tccttcaaca gtcttttatg
     2641 accgggccgt tggtgccccg tatattgaaa tcagactcag gagggaggca atggccagat
     2701 acgggattac ggtaagtgag ctgcaggaag tgctgcaggt tgctgtcggg ggtatgtccc
     2761 tcacaacttc ggttgagggc cgtgaacgat tccccctgag agtgcgctat gcccgtgaac
     2821 tgagggacaa tcctgaagat ctcggaaaga tactgatacc tgctgccggt ggtatccagg
     2881 taccgctcgg ggccgtagca gatattgact atacaaaagg tgcgcagatg atacagagtg
     2941 aaaacacctt ccttgttggt tatgtgattt ttgacaagca tgaggggaaa gcggaggttg
     3001 atgttgtcaa tgaggcagcc gggattctca gggcgggaat cgattccggg gatattgaac
     3061 tgccccccgg ggtaagctat aagtttgccg gcaactatga gcatcagatc agggcaacca
     3121 aaagactatc agtcgtaata cccctcagtc ttatcataat tctttttttg ctctatcaga
     3181 gattccggac gattaccgct tcattcatac atttctcggg agtgtttgtg gcatttgccg
     3241 gaggctttat catgctgtgg ctgtacggcc aggactggtt tatgaatttc aatctggccg
     3301 gaatgaatat gcgtgaactg tttcagatgc acaccataaa tctgagcgtt gccgtttggg
     3361 tcggatttat tgcccttttc ggaattgcga cgaatgacgg ggttctgatg ggcacctata
     3421 ttcatcagat ttttgaggaa aggcagccag ccactgtgaa tgaggtgaga aaggcagtgc
     3481 ttgatgcggg gaagaagcgt gtaagacctg caatgatgac cacagcggtt gccgtgattg
     3541 cattgctgcc ggttctctcg tcaacaggga agggatcgga tataatggtt cccatggcaa
     3601 tacctgcttt cggaggtatg cttatacagg taatgacaat tttcgtcgta cccctgtttc
     3661 aggcgatctg gagagaaacg gaaataaaca agtcagccgc tcccggcccg gcggaaagaa
     3721 ttataaccga aggaactcag taaacaaatc atacatattg aataatgcag gagaaagaaa
     3781 aacatataac gaaaagcggt tctgcaggtt cgggaaataa cagcggtctg attcattcag
     3841 gcaggaaaag ggctctgctt tatattgcct ccttttatgt ctttttattt tcgctttctc
     3901 cactgcattc acaggaggat tcccttaaaa aatatattga gattgccgtt gcgaacaatc
     3961 catccctgct gaaaaaagac agtgaatatg aggcttttca gaaaaaagca gtacaggcag
     4021 gaagtctgcc ggatcctcag cttgatgccg ggctgtttct ttcgccgatg gagatcgtca
     4081 gtggcaggca gtacgcggat cttcgtctga tgcagatgtt ccccttgttt ggtgtgctga
     4141 agaatgcgga agatgagatg aggctgctgg cccgggcgaa acgggaggag tatattgatt
     4201 cggaactgga gcttgtctac aatgtcagac ggacctggta cgagcttttc aggctgagga
     4261 gggagatcgg catttcggaa aagaacctgg agatactc
//
LOCUS       DAOSPX010000285         4291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18548_length_4291_cov_3.259915, whole genome shotgun sequence.
ACCESSION   DAOSPX010000285 DAOSPX010000000
VERSION     DAOSPX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18548_length_4291_cov_3.259915"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(397..2517)
                     /locus_tag="PLZ75_11720"
     CDS             complement(397..2517)
                     /locus_tag="PLZ75_11720"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOS73102.1"
                     /translation="MSAIQFLREKAGVFVAVVIGVALLLFVVSDFFGGGRGQRLQAKK
                     YYQIGEIAGEYISYQDFDRRVQSMLEIYKLSGAASIDEATTESVREQVWQQMVREHIQ
                     DKQYHKLGIGVSTEETEEMVLGNDPHPIVRQLFTDQTTGMFNKSFLVNFLKQTELDET
                     ANRYWLFFEDEIVNERMNSKYNNLVSKGLYVTSRQAEFDRMVSASTVDFSYIMKNYAL
                     VPDSLVRISKSEIESYYSKNKERYRRNALRNIEYVTFSIDPSEEDIRQTEDWIMKTRE
                     EFAEAADPVQFINLTADSRYNSFFVPIGEVPENLREFVRKENKNEIFGPYEEDGAFKL
                     ARLIEAADRPDSVHARHILIAPGQTRDMARAKVVADSLVRLIKSGSRFEVLAMTNSDD
                     QGSAQIGGDLGWFREGMMVTPFNDACFTARKGEILTAETNYGIHIIEILDKSRNTRKY
                     NIGVIDRKIMPSSATNQRIYSAASQFAGTNNTLEKFNNAISEMGLKKLMANDVSPQQK
                     TLPGLENPRSLVMALFSAKEGQIILDNNQQAVFELGDDYVVAFCTRVQEEGIAPLSAV
                     ETEIRLDLMRNRKADIISDEFARINPGNDKSLDELANAMGLTVQEATGINFRSYSVPG
                     AGTEPALVAAASAEKHGVVAGPVKGSNGVFMFFVNGVTTSDGQDLKHVQENMLISYQM
                     RGGYEAYEALRKSASIVDKRYKFY"
     gene            2895..>4291
                     /locus_tag="PLZ75_11725"
     CDS             2895..>4291
                     /locus_tag="PLZ75_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOS73103.1"
                     /translation="MADLRFSALKEVIRREPVVVQTPAKDVSRYFGSNVFNMHQMEHY
                     LSKEAFRFVKKAVTEGKSLTRQEAEQVAIGLKAWALEKGVTHFTHWFHPLTGVTAEKH
                     DGFIEMGDNGHMVENFSGEVLVQSEPDASNFPSGGIRNTFEARGYTAWDVSSPVFIVG
                     TTLCIPTIFVSYTGEALDYKAPLLKALTELDRAAVEVCQYFDKDVNKVIATLGSEQEY
                     FLVDEALYTARPDLVLADRTLMGHQSSKNQQLSDHYFGSIPERVMCFIKDFECEAHKL
                     GIPIKTRHNEVAPNQFECAPIYEEANLAVDHNQLLMDVMRRIARRHRFRVLFHEKPFA
                     GINGSGKHNNWSMATNTGINLLSPGKNPKSNLQFLTFLVNVIKAVHDSNDILRASVMN
                     ESNSHRLGSNEAPPAIISVFLGTYLTSILDELVSKVTDRKMTPEQKTELKLGIGKIPE
                     ILLDNTDRNRTSPFA"
BASE COUNT         1148 a          994 c          925 g         1224 t
ORIGIN      
        1 cataattata atttacttgt agattctaaa caggcgcctc caaaaattaa gttcatactg
       61 cattggctca ttacatggat aatttacaaa attattcata tttggcaaat cccttgttat
      121 aacagttatg gatttgtctc gtgaatgaca ttgtaaaacc aggttattgc atattggcca
      181 acagtaacat acaatgcttt tagcaacata gctgctatga aactttcccc gcctgggtca
      241 ctctgtttca cgcagttgtt cagaaatttt tattcggatt ttaagttgaa agcattcctg
      301 tccgacggat taccggagaa aaggttcaca tccggaaaat tttctcaggg gaaaaacatg
      361 aaccacagct gaacaaaaag ggaggaaaca gatctcctaa tagaatttat accttttatc
      421 aacgattgag gctgatttgc ggagtgcctc ataggcttca taaccgcctc tcatctggta
      481 tgagatcagc atattttcct gaacatgctt aaggtcctgc ccgtcggatg ttgtaacgcc
      541 attgacaaaa aacatgaaga caccattact gcccttgacg ggtccggcga caacaccatg
      601 tttctcggct gatgcagctg caacaagcgc aggttccgtg ccggcaccgg gaacagagta
      661 ggatctgaaa ttgattccgg tggcttcctg tacggttaat cccatggcat tggccagttc
      721 atccagactt ttatcatttc cggggttgat tctggcaaac tcatcggaga taatatctgc
      781 tttcctgttt ctcataagat caagtctgat ttctgtttca accgcactga ggggggcaat
      841 gccttcttcc tgtaccctgg tacagaaagc aacaacgtaa tcatctccca gttcaaagac
      901 tgcctgctgg ttattgtcca gaataatttg tccttctttg gcagaaaaca gcgccatgac
      961 aaggcttctc ggattttcaa gtccgggaag tgtcttttgc tgaggggaaa catcatttgc
     1021 catgagcttt ttaagcccca tttcagaaat tgcgttgttg aacttctcaa gggtattgtt
     1081 tgtacctgca aactggctgg ctgcactgta aattctctgg ttggtggccg aacttggcat
     1141 tattttcctg tcaatcacac caatgttgta tttccttgta tttcttgatt tatcgagaat
     1201 ttcaataatg tgtattccat aattggtttc agctgttaaa atttccccct tccttgccgt
     1261 aaagcaggca tcattaaagg gggttaccat cattccttcc ctgaaccatc ccaggtcgcc
     1321 ccctatctgg gccgaaccct ggtcgtcaga attggtcatt gccagtacct caaacctgga
     1381 acctgatttt ataagcctta ccagactgtc ggccacgact tttgcccttg ccatatccct
     1441 ggtctgaccg ggggcgatga gaatatgcct tgcatgaacc gaatcaggcc tgtccgctgc
     1501 ttcaatcagt ctggccagct tgaaggcccc atcctcttca tacgggccga agatttcatt
     1561 cttgttctcc ttcctgacaa actccctgag gttttcaggc acttcgccga tggggacaaa
     1621 aaagctgtta tatcttgaat cagcagtcag attgatgaac tgaaccggat cagcagcttc
     1681 cgcaaattct tcacgggtct tcattatcca gtcttccgtc tgacggatat cctcttccga
     1741 agggtctatg ctgaaggtaa cgtattcaat attcctgagt gcatttctgc ggtatctttc
     1801 cttgttcttt gaataatagg attcgatttc tgattttgat atccttacca gtgaatcggg
     1861 aaccagggca tagtttttca ttatatagct gaaatccact gtggaggcag ataccatcct
     1921 gtcaaattcc gcctggcgcg aggtaacata caatcctttt gaaaccagat tgttatattt
     1981 tgaattcatc cgctcgttta ctatttcatc ctcgaaaaac agccagtagc ggtttgcagt
     2041 ttcatccagt tcagtctgtt taaggaagtt cacaaggaat gacttgttga acatgccggt
     2101 tgtctgatcg gtaaacaact gccttactat gggatgagga tcgtttccaa gtaccatttc
     2161 ttcagtttct tcagtactga ctcctattcc gagtttatgg tactgcttgt cctgaatatg
     2221 ctcccttacc atctgctgcc atacctgctc ccttaccgac tcggttgttg cctcgtcaat
     2281 ggatgctgca ccggataatt tgtatatctc gagcatggac tgaacccttc tgtcaaaatc
     2341 ctgatatgaa atatattccc ctgcaatttc tcctatctgg taatatttct ttgcctgaag
     2401 tctctgcccc cttccgcctc cgaaaaaatc actgacgaca aaaagcaaaa gagccacccc
     2461 gattactact gcaacaaata cacctgcttt ttcccttaaa aactgaatag ctgacattag
     2521 gtacgctgtt tatggattta gtaataatga tttttaaatt caggcgacaa atataataaa
     2581 attcagtaaa ataaaagcct gaatccggct cactgattat gcaggtttcc ttactttccg
     2641 gaaagcaccc agctttccgg acaatgatat gttttggcga tagttgtttc ttcccggcat
     2701 caataagtat ccggtttctg aagcaatcgt acaaaccata gagcccgccc cggaaataaa
     2761 gagctgatat atccggcagg gccccttcaa aagagggggt aattcaggaa aaaacggtta
     2821 aaaagcagaa tagtcgctta aaatacgggg gtgtattaaa aaatttatat ttttacgcca
     2881 aaatttttaa caagatggcg gatttaagat tcagtgcact gaaggaagtt atcaggagag
     2941 agcctgtggt ggttcagact cccgcaaagg atgtttcgag atatttcggt tccaatgtgt
     3001 tcaacatgca tcagatggaa cattatcttt caaaggaggc tttccgcttt gtaaagaaag
     3061 cagtcacgga gggcaagtct ctgacaaggc aggaagctga acaggttgct attggtctaa
     3121 aggcctgggc acttgaaaaa ggtgttacac atttcactca ctggtttcat ccgctgacag
     3181 gcgtaacggc tgagaaacat gacggattta ttgaaatggg agacaacggc cacatggtgg
     3241 aaaacttctc aggagaggtc cttgttcagt ctgaacccga tgcatcaaac tttcccagcg
     3301 gcggaataag aaatacattt gaagcgcgtg gctataccgc ctgggatgtg tcttcacccg
     3361 tattcattgt cggaacaacc ctttgcatcc cgaccatttt tgtttcctat accggtgagg
     3421 ctctcgatta caaggcaccc ttgctcaaag ccctcactga acttgacagg gctgctgtgg
     3481 aagtgtgcca gtattttgac aaggatgtga acaaggtgat tgcaacgctc gggtcagaac
     3541 aggaatattt cctcgtggat gaagccctgt atacggcacg tcccgacctg gtgctggccg
     3601 accgtaccct tatgggacat cagtcatcaa agaaccagca gctgtctgat cattatttcg
     3661 gatcgatacc tgaaagggtg atgtgtttta taaaggattt tgaatgtgaa gcccataagc
     3721 ttggaattcc aattaaaacg cgtcataatg aggttgctcc caatcagttt gaatgtgccc
     3781 caatttatga ggaagccaac cttgccgttg accacaatca gcttctgatg gatgttatga
     3841 gacggattgc ccgcaggcac cggttcaggg tactgtttca tgaaaaacca tttgccggca
     3901 taaacggatc ggggaaacac aataactggt caatggctac caatacaggt ataaaccttc
     3961 tttcgcccgg gaagaatccg aaatcaaacc tgcagttcct gaccttcctt gtgaatgtga
     4021 tcaaggctgt gcacgacagc aacgatattc tgagggcatc cgtaatgaat gaatcaaatt
     4081 ctcaccgtct cgggagcaat gaagcaccgc cggcaattat ttcggttttc ctcgggacat
     4141 acctgaccag catacttgac gagctggtca gcaaggtgac tgacaggaag atgactccgg
     4201 aacaaaagac cgaactgaag cttggtatcg gaaagattcc ggagatactg cttgacaata
     4261 ccgaccggaa tcgtacctcg ccctttgcat t
//
LOCUS       DAOSPX010000286         4287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18573_length_4287_cov_3.676512, whole genome shotgun sequence.
ACCESSION   DAOSPX010000286 DAOSPX010000000
VERSION     DAOSPX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18573_length_4287_cov_3.676512"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..946)
                     /locus_tag="PLZ75_11730"
     CDS             complement(137..946)
                     /locus_tag="PLZ75_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS73104.1"
                     /translation="MKKTIIVSLTFFAALMSFGQQAPQRSASAGRPSGTQEYPARMTP
                     QMTEIMDPEVKVIQPGEQTGAAPSDAIVLFDGTAGIINREWEDARGNPTRWILQEGAL
                     VCVKGSGVIRTRRKFTDFQLHIEWRAPAEIEGEGQGRGNSGVFLQGLYEVQILDSYNN
                     RTYRNGQAGSVYKQYAPLVNACRKPGEWQSYDIIYTAPRFGNDSASYFTPPRVTVLHN
                     GVLIQNNVSLRGPTLYIGMPEYSIKKHGPEPLVLQDHGNPVAFRNIWIREL"
     gene            1131..1604
                     /locus_tag="PLZ75_11735"
     CDS             1131..1604
                     /locus_tag="PLZ75_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73105.1"
                     /translation="MESKYLSLEPHDQHGIMKVLQIIFGAVCIIVSFYYFIYGSGSDK
                     SGIKLWIAIFFLLLFGMYQIMAGLGKTRKYFITMHGGINYKQHSFLPGVKIAAGEIDK
                     ITFHPLSIVFQLKKGNRQRFRFGISYPEVIDPVKQNVIEFAESHNISFEIVEDEI"
     gene            complement(1621..2640)
                     /gene="yiaK"
                     /locus_tag="PLZ75_11740"
     CDS             complement(1621..2640)
                     /gene="yiaK"
                     /locus_tag="PLZ75_11740"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF009750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HOS73106.1"
                     /translation="MSETNQSIRIQSGKMKAEFFRILKKHGFNDVKAEKCAEIFTVNS
                     LEGVYSHGVNRFPAFVFNVAAGHIKPDADPSLVHSRGSIEQWDGNLGPGPLNALFAAD
                     RAVALAGKSGTGLVGLANTNHWQRGGTYGWHAAKKGFIMICWTNTIANMPAWGASDAR
                     LGNNPFVMALPYENEAIVLDFSMTQYSYGKMETYSREGKRLPYAGGFDEMGALTDDPA
                     EIIRTQRALSIGFWKGAGFSFLLDILASLLSGGLSTHEISESKSEYSVSQVFMSINPG
                     DLYNFPSIDETIRDIIADLKKSVPAGDASLIRYPGENILRIRSDNQKKGIPVNAELWN
                     QIVSL"
     gene            complement(2709..3959)
                     /locus_tag="PLZ75_11745"
     CDS             complement(2709..3959)
                     /locus_tag="PLZ75_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS73107.1"
                     /translation="MKKSLIVLFCAVPVFLPGYPVSGQDTDSWKASPGIVEKLSKARS
                     DVNYYEEKVPAYVLPDLLLSSSGRKINTASAWMKQRRPEVLELFRENVYGRIPPTPYT
                     KTFRLVNEDSNAMNGMATLKEIDIVIGSEGKSLTIHLYIFLPNNEKKPVPLFLLINNR
                     DPSNIDPSRKVRSEFWPAEEVIARGYGIAAFYNADVDPDKYDEFRDGIHGILDRGRGA
                     DSWGTIAAWAWGASRCMDYFVSDRDIDDKKIAVAGHSRGGKTALWAGAEDQRFALVIS
                     NESGCGGAALARRKFGETIARINGSFPHWFCTNYRKYNDNEDSMPVDMHMLMALIAPR
                     AVYVASAEEDLWADPRGSYLSLYHSMPVFQLLKTKTSLPEAMPPLNKPVYSGKAAYHI
                     RDGSHNMLLKDWNWFMDFADTLFK"
BASE COUNT         1136 a          999 c          919 g         1233 t
ORIGIN      
        1 acaattttaa tcattgttat caactgataa caagacttct gtaattcaga aaaggaaaac
       61 aagttgcttt gcatgcaatg atgccgggaa tcgcttcttt ttcttcccat tttttaaaat
      121 catcaggaaa gataaatcag agttctctta tccagatatt tcggaaagca accggattgc
      181 catgatcctg aagaaccagt ggttccggtc cgtgtttttt tattgagtat tccggcatcc
      241 cgatatacag ggttggtccc ctgagactga cattattctg aatgaggaca ccgttgtgca
      301 gtaccgtgac tctgggaggt gtaaaatatg aagctgaatc atttccaaac cttggggcag
      361 tatagattat atcatagctc tgccactcgc cgggtttcct gcatgcatta accaggggtg
      421 catattgttt gtaaacgctt cccgcctgcc cgttgcggta ggttctgtta ttatagctgt
      481 ccagtatctg gacctcatac aagccctgca ggaaaactcc gctgtttccc ctgccctggc
      541 cttccccctc aatttctgca ggagctctcc attcaatatg caactgaaag tctgtaaact
      601 tacgcctggt acggatcact ccggaccctt tcacacacac cagggctcct tcctgcaata
      661 tccatctggt aggattcccg cgggcatctt cccattcccg gttaataatc ccggcagtgc
      721 cgtcgaaaag gactatggca tcggaaggcg ccgctccggt ctgctcaccc ggctggataa
      781 cctttacttc cggatccatt atttcggtca tttgtggtgt catccttgcc ggatactcct
      841 gggtcccgga gggtctgccg gcagaagctg atctctgagg ggcctgctgg ccaaagctca
      901 tcagagccgc aaaaaatgtc aagctgacaa taatcgtttt tttcatgctg ttcagattta
      961 atatgtaatg gtaatttcag tattataatc cgccggagag caaattatta aattatgagg
     1021 gaaaatgaaa atgccggaag cggataatta aggccaaagt tcaaaaatac ggattttcag
     1081 taattttgcc attcagcaaa aaaaatataa atttcccgaa acaagaactg atggaaagca
     1141 agtatttgag tcttgaacct catgatcagc atggtataat gaaagtgctt cagattattt
     1201 tcggtgcagt atgcattatt gtatcctttt actatttcat atacggaagc gggtctgaca
     1261 agtcgggaat aaaattatgg attgccattt tttttcttct tctttttggc atgtatcaga
     1321 taatggctgg ccttggaaag accagaaagt attttataac aatgcacggt ggaataaact
     1381 acaaacagca ctccttcctt ccgggcgtaa aaatagcagc cggcgaaata gacaagataa
     1441 catttcatcc cttaagcatt gtatttcagc ttaaaaaggg aaacagacag aggttccgtt
     1501 tcggtataag ctaccctgag gttattgatc cggttaagca gaatgtgatt gagttcgccg
     1561 aatcccataa tatttccttt gagattgtgg aggatgaaat atagccggga agcagataaa
     1621 tcacaggctc acaatctgat tccataattc tgcattcacc gggattcctt ttttctgatt
     1681 atctgaacgg attctgagta tattttcccc ggggtaacgg atcaaagagg cgtcaccggc
     1741 aggtactgat ttcttaagat ctgctatgat atcccggatt gtctcatcaa ttgaagggaa
     1801 attatataag tcgccgggat ttattgacat aaagacctgg gatacactgt attcggattt
     1861 gctttcactt atttcatgcg tggaaagtcc tcctgataaa agcgatgcca ggatatcaag
     1921 cagaaaagag aatccggctc ctttccagaa tccgattgag agtgcccgct gtgtccggat
     1981 gatttctgcg ggatcatccg tgagtgcccc catttcatca aagcctcctg catagggcag
     2041 cctttttccc tccctgctgt aggtttccat tttcccatag gaatactggg tcattgaaaa
     2101 atccagtaca atggcttcat tttcatatgg cagggccatt acaaagggat tgtttcccag
     2161 cctcgcgtct gatgcccccc atgccggcat atttgctatc gtattggtcc agcatatcat
     2221 gatgaatccc ttttttgcag catgccagcc ataggtccct ccccgctgcc agtgattggt
     2281 attagcgaga ccaacaagac ctgttccgga ttttccggcg agagccacgg ccctgtcagc
     2341 agcaaaaagg gcattgagag gtcccggtcc gaggttgccg tcccactgct caatggatcc
     2401 cctgctgtga acaagggacg gatcagcatc cggtttaata tgtccggcag ccacgttgaa
     2461 cacgaaagcg ggaaatctgt tgactccatg tgaataaaca ccttcgagac tgttaacagt
     2521 aaatatctcc gcacattttt cagcctttac atcattaaag ccatgttttt tcagaatccg
     2581 gaagaattcc gctttcatct ttccggattg aattctgatg ctttgatttg tttccgacat
     2641 tgagtataaa taaaaatgca tgtactatcc cggacaggag gaaagcccgg agaattcctg
     2701 aagaaggttc atttaaaaag cgtatctgca aaatccatga accagttcca gtctttcagc
     2761 agcatattat gacttccgtc acgaatatgg taggctgcct ttccgctgta aaccggcttg
     2821 ttcaggggcg gcatcgcttc tggcagtgag gtttttgttt tcagtaactg aaaaacaggc
     2881 atggaatgat agagtgaaag atatgatcca cgcggatctg cccacagatc ttcttcagct
     2941 gaagccacat atacggcacg aggagctatc agtgccatca gcatatgcat atctacgggc
     3001 attgaatctt cattatcatt atatttccgg taatttgtac aaaaccagtg aggaaatgag
     3061 ccgttgattc ttgcaatggt ctcgccgaat tttctgcggg ccagagccgc tccgccacaa
     3121 cccgattcat tactgatgac cagtgcaaat ctttgatctt ccgcaccggc ccagagtgct
     3181 gttttgccgc ctcttgaatg gcccgccaca gcgatttttt tatcgtcaat atccctgtca
     3241 ctcacaaagt aatccatgca cctgcttgct ccccatgccc atgctgcaat tgttccccag
     3301 gaatcagctc ctcttcccct gtcgagtatt ccatgaattc catccctgaa ctcatcatat
     3361 ttatccggat ccacatccgc attataaaat gctgcaatgc catagccacg ggcaataact
     3421 tcctcagccg gccagaattc gcttctgact tttctcgacg gatcaatatt tgacggatcc
     3481 ctgttattta tcagcagaaa aagaggcacc ggcttttttt cattgttggg caaaaatata
     3541 taaagatgaa ttgtgagaga ttttccttca gatccgatga ctatatcaat ttctttcagg
     3601 gttgccatgc cgttcattgc attactgtcc tcattcacca gcctgaaggt ttttgtatac
     3661 ggagtcggcg gaatccttcc atatacattt tccctgaaaa gctcaagcac ttcgggacga
     3721 cgctgtttca tccaggcaga tgcagtattg attttccgtc ctgaggaact cagaagcagg
     3781 tcaggaagaa cataggcagg aactttttct tcataataat tcacatcgct gcgtgctttc
     3841 gaaagcttct ccacgatccc gggggatgct ttccagctat ctgtatcctg gcccgaaacc
     3901 ggatagcccg ggagaaagac cggaacagca cagaataaga caataaggct ttttttcatc
     3961 actttctttt ttctttattt tgatatgttt tgccgggcag gaaaaaaccg gtttaaattt
     4021 tttatgatac taagttatag tttttgccca gaagaccaaa aaagatttat cctgataacg
     4081 ggagtccgtc ggcggtgcaa tggaaatctt aagagtcctt tggatttact cgtggtttat
     4141 atgtaatttt atcagcctgt tcctgccaat acatcatgaa cacattatac agacgacagt
     4201 cgggagataa aatccagtgt atcctctgcc ctcacaattg cataattgca gaggggagga
     4261 ccggtatttg cggtgtcaga aaaaaca
//
LOCUS       DAOSPX010000287         4275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18636_length_4275_cov_4.755687, whole genome shotgun sequence.
ACCESSION   DAOSPX010000287 DAOSPX010000000
VERSION     DAOSPX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18636_length_4275_cov_4.755687"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLZ75_11750"
     CDS             complement(<1..549)
                     /locus_tag="PLZ75_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73108.1"
                     /translation="MIWSIAWKNVWRNRKRSLVVIVAVSLGITGGVFLFGFVEGWSRQ
                     RMDNAIYNEVSHIQIHNSEYRKNEEIGLTVQNLNGLSRSIDTLRELSAWTKRTKIIAM
                     LETPWGNTGLKLYGINPEKEKRISRIHNMIVRDGGRYIDKKNPSDILISDKTADMLKL
                     KHYIISDTVIAKLTGGKIPERII"
     gene            complement(563..1984)
                     /locus_tag="PLZ75_11755"
     CDS             complement(563..1984)
                     /locus_tag="PLZ75_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOS73109.1"
                     /translation="MNGEFRMAWRNLWRNRRRTILTAASVSFAVFFALVMRSIQLGSY
                     DHMINNFIGSFTGYLQVQHVKYLDNPIIDNSFNYDTSLIAAISSLDNVVSVTSHLETF
                     TLASGGTQTKGVAVIAIDPEREKYFSDPEGKLVKYRISDESVRKLQDEGLIPEKVLDI
                     LRKNADRSYSSLKRLELELGLDEEETTLFMPEILKHTGFANGYLTDDDDGVLVSDRLS
                     AFLNISIGDSVILMGQGYHGVSATGIFPVRGIIKIPAPDIDNKLIIMTIPAAQVLFDA
                     EGKISSLVINLTDRSERTIRHTRNRINSFLHDRNITTRTWYELNPVLYQQIKGDSQGS
                     LLILAVLYFIIFFGIYGTVLMMVSERRKEFGVLVAIGMQKGKLRRVVSIEMTLLGVVG
                     LLAGMLASLPLVIYLNNYPLFLTGELGNMMEGWGWDAIIATALPGSYYCWQALIVMIM
                     VLLAAIYPLRKIRKLKEIEALKG"
     gene            complement(2031..2795)
                     /locus_tag="PLZ75_11760"
     CDS             complement(2031..2795)
                     /locus_tag="PLZ75_11760"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOS73110.1"
                     /translation="MGKLIRLIFLLILFPGGNIILSAQSLTAKEVVRRAEEKFRGEKS
                     SYAEMSLKVIRPEWQRTIEFKTWAMGSGFAISLITSPPREAGQSFLKRGSEMWSWNPS
                     ISRIIKLPPSMLSQGWMGSDYTHDDILKETSAVNDYSHEITGEEEIEGRMCYKIEMTA
                     LEKASVIWGKQVRWIDRQDFLVLKAELYDEDGYLVRTETCSDIRIMDGRSITSKIELV
                     PEDEPRNKTVLEIRKIRFNIPVEESFFSQQNMKRLH"
     gene            complement(2870..3532)
                     /locus_tag="PLZ75_11765"
     CDS             complement(2870..3532)
                     /locus_tag="PLZ75_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS73111.1"
                     /translation="MAPKSPQQLKEIREEKKAVIMDSALLLFAGRGFHATTIGDIAEG
                     ADISKGLMYNYFDGKEDLLAEIIRTSLAGISRSLDADIPYISHDRPLSPDEFGLIVRR
                     LGRLVREKSTFLKLLFQVFMQPLVREQLSGIFREGSPAGREGEESGETVFLSRLMKSI
                     RGYFERKRALPGDEHDPGMSTEMFLLTMKGFALTYVFMDPENFDSDSFEKAVDEIIDL
                     YK"
     gene            3935..>4275
                     /locus_tag="PLZ75_11770"
     CDS             3935..>4275
                     /locus_tag="PLZ75_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73112.1"
                     /translation="MASLKLILGLIPSTTKLELNEKALTDEYEKLRAFTDSELLARYN
                     KLNDLINSPDFQRKKKEIESLQYKNSEEYAREKEFLSLQKSRDIVLYFKTAAGSALKK
                     FREMDGSAAIR"
BASE COUNT         1146 a         1037 c          801 g         1291 t
ORIGIN      
        1 aataatcctt tcaggaattt ttccccctgt cagtttcgcg ataacagtgt cactgataat
       61 ataatgcttt agtttgagca tatcagcagt tttgtcactg ataagaatat cagagggatt
      121 ttttttgtca atatatctgc caccatcccg gacaatcata ttatgtatcc ggctgatccg
      181 tttttctttt tccggattga taccatacag tttcagtcct gtattccccc acggggtttc
      241 aagcattgca attatttttg tccttttggt ccaggcggaa agctccctca gtgtatcaat
      301 gctccggctt aatccgttga ggttttgcac agtcagacct atttcttcat ttttccggta
      361 ttcactgtta tgaatctgaa tatgggatac ttcattataa atggcattat ccatgcgctg
      421 ccggctccag ccttcaacaa agccgaaaag aaatactcct ccggtaatac ccaggcttac
      481 ggccactatt acaacaaggc ttcttttcct gttgcgccat acattcttcc atgctattga
      541 ccatatcatc aagtgtccgg ttttaacctt taagtgcttc aatttccttt aattttctta
      601 ttttcctcag gggatatatg gcggccagta acaccattat catgacaatc aaagcctgcc
      661 agcaataata ggaacccggc agggcagttg ctattatggc atcccatccc catccttcca
      721 tcatgtttcc cagctctccc gtgagaaaaa gcgggtaatt attaagataa atcacgagcg
      781 gcagactggc aagcattccg gccagcaggc caacaacacc aagcagtgtc atttcaattg
      841 aaactactct cctgagtttt cccttctgca tccctatggc tacgagaaca ccgaattcct
      901 ttctcctttc cgataccatc atgagcacag tcccgtagat gccgaagaaa ataatgaaat
      961 acaggacagc cagaataagc agtgagccct gactgtcacc ctttatctgc tggtacaata
     1021 cggggtttag ttcataccat gtcctggtcg tgatattcct gtcatgaaga aaggaattga
     1081 tcctgtttct tgtatgcctg attgtgcgtt ccgaccggtc tgtgagatta atgacaagcg
     1141 agcttatttt gccttcagcg tcaaatagta cctgggcagc cggtattgtc ataatgatta
     1201 atttgttatc tatgtcaggt gcaggtattt taataatccc gcgtacagga aaaattccgg
     1261 ttgccgaaac accatgatat ccctgtccca tcagtattac cgaatcaccg atgcttatat
     1321 tcaggaaggc cgatagccgg tctgaaacca ggactccgtc atcatcatct gtaaggtagc
     1381 cattggcaaa tccggtatgc ttaagtattt ccggcatgaa aagtgtcgtt tcctcctcgt
     1441 ccagcccgag ttcaagttca agccgtttta aagatgaata ggatctgtca gcattctttc
     1501 tgagaatgtc cagtactttt tccgggatca aaccctcatc ctgcagtttc ctgactgatt
     1561 catcacttat cctgtacttt acaagtttcc cttcaggatc ggaaaaatac ttttcccgtt
     1621 caggatcaat ggcaataaca gccacacctt tcgtctgtgt gccgcctgaa gccagtgtaa
     1681 aagtttcgag atgggatgta acggaaacaa cattatcaag cgaggaaatt gcagcaataa
     1741 gtgaggtatc atagttgaat gaattatcaa ttatggggtt atccagatac ttaacatgct
     1801 gaacctgcag gtagccagta aatgacccta tgaaattatt gatcatatga tcataggagc
     1861 caagctgtat ggatctcatg acaagtgcaa agaacaccgc aaaggaaacc gatgctgcgg
     1921 tcaatatggt ccgccgcctg ttcctccaca gattcctcca tgccatcctg aattccccgt
     1981 tcatagggat agtcctgatt aaaaaaatca catatccgtt ttgctgcaga tcaatgcagc
     2041 cttttcatgt tctgctggga aaaaaagctt tcctccacag gaatattgaa tctgattttc
     2101 ctgatctcaa gtactgtttt gttccgtggt tcgtcttcag gaaccagctc aatttttgaa
     2161 gtaatgcttc tcccgtccat gatcctgata tcagaacacg tttccgttct tacaaggtaa
     2221 ccgtcttcat catataattc tgcctttaac accagaaaat cctgcctgtc aatccatctt
     2281 acctgttttc cccatattac cgaagctttt tcgagtgcgg tcatttctat tttataacac
     2341 atccgtcctt caatctcctc ctctcccgtg atttcatgac tgtaatcatt tacagccgac
     2401 gtttccttca ggatatcgtc atgggtatag tcagatccca tccagccctg cgaaagcatt
     2461 gaaggaggaa gttttattat tctgctgatg gaagggttcc agctccacat ttcagaacct
     2521 cttttcagaa aagactgccc tgcttccctg ggcggagaag taatgagtga aattgcaaac
     2581 ccgcttccca tggcccaggt tttgaactct atcgttctct gccattcagg tctgatcacc
     2641 ttcagtgaca tttcagcata actgcttttc tctcctctga atttctcttc agcccgtctg
     2701 accacttcct ttgcagtaag agactgggca gaaaggataa tattaccgcc cgggaacagg
     2761 atcaacagga atatcagcct gataagtttt cccatttttc tttcttttaa cgtcgtttct
     2821 actgattcaa cttacacttt gcgttctgtc cgggccggat gtgcagggat tatttataaa
     2881 gatctattat ttcatcaact gccttttcaa aagaatcact gtcgaaattt tccggatcca
     2941 taaaaacgta agtcagtgca aatcccttca tagtgagaag gaacatttcc gtactcatcc
     3001 ccggatcatg ttcatccccc ggcaatgccc ttttcctttc aaaatacccc ctgatgctct
     3061 tcatcagccg ggacaggaag acagtttccc cggattcttc cccctccctg ccggccggac
     3121 tgccttccct gaaaataccg gaaagctgct ctctcaccaa aggctgcata aaaacctgga
     3181 aaagaagttt caggaatgtg cttttttccc tgaccagccg gccaagcctg cggactatca
     3241 ggccaaattc atccggggat aacggcctgt catggcttat gtaaggaata tcagcatcaa
     3301 gcgagcggga tatcccggcc agtgatgtcc tgattatttc tgccagaaga tcctcctttc
     3361 catcaaagta attatacata agtccctttg atatatcagc cccttcggca atatcaccta
     3421 ttgtggttgc atgaaatccc cggccggcaa aaagcaacag tgctgaatcc attattactg
     3481 cctttttctc ctctcttatc tccttaagct gttgtggtga ttttggcgcc ataattcttt
     3541 gactgaacgt ttattcaatt tatattattt ttattatcaa ttatttttaa ctatgactga
     3601 acattcattc aagactattt cctgtttcgt tttccttatg acagaatatt cattcaaggt
     3661 catttccttt tttcgttttt cttatgactg aacattcagt cagtatcaat gtttcaaaaa
     3721 accgttccgt gaacggaacg attctgctaa agataaaatg aaaaccgccc ggaagcaaat
     3781 tttaattatc cgccctgtcc gattattatg cagtctcctg caacattcac cgcatatctt
     3841 ccgttaaacc agaaatctga tatttgaata caggttctta aattattaag aaaagatttc
     3901 tatctttata cgctgtatct aaaatgatta accaatggca agtctgaagc ttatactcgg
     3961 attaattccc tctacgacaa aactggaact aaacgaaaag gccttaactg atgaatatga
     4021 aaaactaagg gcctttactg attccgaact cctcgcccga tacaataaac tgaacgatct
     4081 tatcaattcg ccggatttcc agcggaagaa aaaggaaata gaatcacttc agtacaagaa
     4141 ttccgaagaa tatgcccggg agaaagagtt tctttccctt cagaaatcca gggacattgt
     4201 gctttatttc aaaactgccg cgggaagtgc gctgaaaaag ttccgggaga tggacgggtc
     4261 tgcggcgatc aggga
//
LOCUS       DAOSPX010000288         4250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18754_length_4250_cov_5.488200, whole genome shotgun sequence.
ACCESSION   DAOSPX010000288 DAOSPX010000000
VERSION     DAOSPX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18754_length_4250_cov_5.488200"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLZ75_11775"
     CDS             complement(<1..198)
                     /locus_tag="PLZ75_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73113.1"
                     /translation="MNQNEQKFIKIQLQKNHPIQQLLFTFGLHKRLAFSGKNTDTIKQ
                     RQQTHLRSLRTKEGKTMKKLFT"
     gene            complement(363..436)
                     /locus_tag="PLZ75_11780"
     tRNA            complement(363..436)
                     /locus_tag="PLZ75_11780"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(400..402),aa:Ala,seq:ggc)
     gene            645..1445
                     /locus_tag="PLZ75_11785"
     CDS             645..1445
                     /locus_tag="PLZ75_11785"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOS73114.1"
                     /translation="MALFNGTPGFDEIVFASRNRDYGAYKLRKRYFSALLTGLISAIL
                     IACIVVLIPFFSKPPEDEIFPGGGRYISIMMDRLDAPSDQIYVPPPPAAPSRPVPKAA
                     QEIVRYVAPEVIDTIIPSVQTPLSTDELIIQSGEGTDDNEGFGFGDEIFDGHGSGGYY
                     TDEPFFLVEVMPSFRGGDINRFREWVQKRTNYPQEALDKKIQGRIFLTFIVEADGAVS
                     NVTVVKGVDPLIDDEAVKAIAASPKWTPGLQRGQPVRVRYSLWLSFVI"
     gene            1535..2707
                     /locus_tag="PLZ75_11790"
     CDS             1535..2707
                     /locus_tag="PLZ75_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73115.1"
                     /translation="MKRSVIFVLTLTILLQGCGSSKKQFERGNYDAAVTSAVRQLRKK
                     PDDTKQITTLERSYTIANEQDLERVRFLKMENNPRNYDEIYQIYQRLNDRQSLVRTVL
                     PLRSGSRTIDFPYVDYMEEMIAAKRKSADFYYAHGMDLMKNGLKDSYRQAYYEFVRAK
                     EYVGDYEGIDNKIAEAKYLGMSRVLVSLQNSSMIRFPQEFEDDLLNVNLQSLNSEWIE
                     YHTVSYDEKTKYDYFINVNVRHILVSPDQTSQVDSVIKREVEDGFIYQLDKRGNVMKD
                     SLGNDIKTKKYKTLQCALISTLQTKSCQIEGDIETIQINPDKLLKKDPIGAHSIFEHV
                     SARALGDVAALSPGQLARTKSQVIPFPSDIEMILRCSESLKNAIRGSVQSNRRLII"
     gene            2712..3902
                     /gene="hflX"
                     /locus_tag="PLZ75_11795"
     CDS             2712..3902
                     /gene="hflX"
                     /locus_tag="PLZ75_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOS73116.1"
                     /translation="MIETGIPAERAVLVGINYPGQDENDTNEFIDELAFLAETAGAVP
                     VERFIQKLNIPNAATFVGSGKTAEIAGFVEANSIDLAIFDDELSPVQIRNLENALQCR
                     VIDRTNLILDIFARRARTSHARKQVELAQYQYLLPRLRRMWTHLERQRGGIGLRGPGE
                     TELETDRRIIRDKIALLKEQLKKIDVQMATQRKNRGKLVRVSLVGYTNVGKSTLMNLL
                     AGSEVFAENKLFATLDTTVRKVVIRNLPFLLSDTVGFIRKLPHDLVESFKSSLDEVRE
                     ADLLLHIVDIAHPRFEEQIDVVNETLLELGSMNKPAIIVFNKTDIFSHVPKDEDDLTP
                     LKKENYSLEDLKKTWMAKNMNYPTVFISARTGQNMDELRKILYDRVREIHVTRYPYND
                     FLFR"
     gene            complement(3865..>4250)
                     /locus_tag="PLZ75_11800"
     CDS             complement(3865..>4250)
                     /locus_tag="PLZ75_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73117.1"
                     /translation="TFFADNIKRMYAAGSLEKFSSELERSREIASTIPDKAVISVLNG
                     MMARPSRLSVMEGGKVPLLWILGAGDNYIDYRQIQTRVKLPPNAEVFVLENSGHMGFI
                     EEEAEALKKLSEFVTGTGSHYRDNG"
BASE COUNT         1219 a          960 c          996 g         1075 t
ORIGIN      
        1 tgtaaataat tttttcattg ttttgccctc cttagttctt aatgatctta aatgtgtttg
       61 ttgacgttgc tttattgtgt cagtgttttt tccactgaat gctaatcgtt tatgtaaacc
      121 aaaggtaaac aataattgct gaatcgggtg gtttttttgc agctgaattt ttatgaattt
      181 ttgctcattt tgattcatct gttaaaaagg tttttcactg taatatttga aattgattac
      241 cagacaggcc agattgtaat gtacagttac aaacttttta ttgcgggaat ctcccaggca
      301 gcaattaaaa taaaaaacca cccggctgaa ggggtggttc cgcatgctat aatcatgatt
      361 tgtggagatt aggggaatcg aacccctgac cttttgactg ccagtcaaac gctctagcca
      421 actgagctaa acccccggaa aaaaagcctg tctgatccca tccgcttccg gcttctgaaa
      481 cttctcccgg atccctcagt acggaaccgg ccggaacaga ctctgatttt gagccggaca
      541 aagttatata aaatatcaat attataaaac atacttgccg ggctcatcct ccgttttgtc
      601 agtaatccgc tattttgtaa ttttgcagga cattttaaac aggaatggct ttgttcaatg
      661 gtacaccggg ttttgatgag attgtattcg ccagccgtaa cagggattat ggcgcataca
      721 aactcagaaa aagatatttc tcggccctgc tcacgggact gatatctgcc attcttattg
      781 catgtatcgt ggtgctgatc cctttcttct ccaaaccgcc ggaggatgaa atattcccgg
      841 gcggaggcag atatatcagt ataatgatgg acaggctcga tgctccctcc gaccagatct
      901 acgttcctcc tcccccggca gctccttcac gtcccgttcc gaaagccgcc caggaaatag
      961 tcagatatgt tgcccccgag gttattgata ccataatacc ctctgtacag actcccttgt
     1021 caacagatga actgattatc cagtcggggg aaggcacaga tgataatgaa ggtttcggct
     1081 ttggtgacga aatatttgac gggcacggaa gcggaggcta ttacaccgat gagccgttct
     1141 tccttgtgga ggtgatgccc tcattcaggg gaggcgatat aaaccgcttc cgcgaatggg
     1201 tccagaaaag gacaaactat ccgcaggaag ctctcgacaa gaaaatacag gggaggatct
     1261 tccttacctt cattgtcgag gccgacggcg cagtcagcaa tgtaacagtt gtaaagggtg
     1321 tcgatccgct tattgatgat gaggcagtca aagccattgc tgcttccccg aaatggactc
     1381 cggggcttca gagagggcag cccgtaaggg tgagatattc gctctggctc agttttgtta
     1441 tctgattacc ttaaactgtg gacaagaacc cgtttttcca ttccctttta attaatgttt
     1501 ttcttatatt tgagctggtc taagtcagtg caatatgaaa agaagcgtaa tatttgtttt
     1561 aacacttaca atcctcctcc agggatgcgg atcctcaaaa aagcagtttg aaagaggtaa
     1621 ttatgatgct gcagtaacaa gtgctgtcag gcaactgcga aaaaagccgg atgacacgaa
     1681 acagatcact accctcgaac ggtcatacac aatagcaaat gaacaggatc tcgaaagggt
     1741 gagattcctc aaaatggaaa acaatccccg gaactatgac gagatatacc agatatacca
     1801 gaggctgaat gacaggcagt ccctggtcag gacagttctt ccgctgcgct cggggagcag
     1861 gactatcgat ttcccgtacg tggactatat ggaggaaatg atagccgcca aaaggaaatc
     1921 agccgatttc tattatgcac acggtatgga tctgatgaag aacggactca aggattcata
     1981 ccgccaggca tactatgagt ttgtaagggc aaaggaatat gtcggcgact atgaaggaat
     2041 agacaataag atcgccgagg caaaatacct gggtatgagc agggtccttg tcagcctgca
     2101 gaacagttca atgataaggt tccctcagga atttgaggat gatctcctga atgtcaatct
     2161 tcagtcactg aacagtgaat ggattgaata ccacacagtg agctacgacg aaaagactaa
     2221 atacgattat tttatcaacg taaatgtcag acatatactt gtcagccccg atcagacttc
     2281 ccaggttgat tcagttatta aaagggaggt tgaggacgga ttcatctatc agcttgacaa
     2341 aaggggaaac gtaatgaaag acagtctggg gaatgatatc aagacaaaaa aatataaaac
     2401 actgcaatgt gccctgatat ccacacttca gacaaaatcc tgccagatcg agggggacat
     2461 tgagacaata cagataaatc cggataaact tctgaaaaaa gatcccatag gcgcccattc
     2521 aatatttgaa catgtttctg caagagccct cggcgacgtg gctgcactca gtccgggaca
     2581 gctggccagg acaaagtcac aggtcatccc ctttccttcg gatatagaaa tgatactcag
     2641 atgttcggaa tcgcttaaga atgccataag gggatcggtc cagagcaaca ggaggctgat
     2701 catttagatt gatgatagaa acaggtatcc cggcggaaag agcggtactg gtaggtatta
     2761 attaccccgg tcaggatgag aatgatacca atgaatttat tgatgaactg gcttttcttg
     2821 ccgaaactgc cggagcggtt ccggtggaac ggtttattca gaagctgaat attcccaatg
     2881 ccgcaacctt cgtaggttcg ggcaaaacag cggaaatagc cggatttgtt gaggccaaca
     2941 gcattgatct tgccattttc gatgatgaac tctcaccggt acagatccgt aatcttgaga
     3001 acgctctcca gtgccgggta attgaccgga caaacctgat actggatatt tttgcccgca
     3061 gggccaggac atcccatgcc cgtaaacagg ttgaactggc acagtaccag tatcttcttc
     3121 ccagactgag gcggatgtgg actcacctgg agcggcaaag gggaggaata ggcctgagag
     3181 gacccggtga aacagagctt gaaaccgacc gcaggatcat ccgggataag atagccttgc
     3241 tgaaggagca gctgaagaag atagacgtac agatggcaac ccagcggaaa aaccgcggca
     3301 agcttgtcag ggtgtcactt gtcggataca ccaacgtggg gaaatcaacc ctgatgaacc
     3361 tcctggccgg atcggaagta tttgccgaaa acaaactgtt tgcaacactc gatacaaccg
     3421 tccgtaaggt ggttatcaga aatcttccct tcctgctttc cgacacggtg ggtttcatcc
     3481 gcaagcttcc ccatgatctt gttgagtcgt tcaaatcaag tctcgacgag gtccgggagg
     3541 cagacctcct ccttcacata gtggatatag cacatccgcg ctttgaagag cagatagatg
     3601 ttgtgaacga gacactgctc gaactcggtt ccatgaacaa gcccgcaata atcgtattta
     3661 acaagacgga tatcttttcc catgtgccga aggacgaaga cgatctcact ccgctgaaaa
     3721 aagagaatta ttccctggag gatctgaaga agacatggat ggcaaaaaac atgaactacc
     3781 cgacagtatt catttctgca agaaccgggc aaaacatgga tgaactaaga aagatactgt
     3841 acgacagggt aagggagata catgttaccc gttatcccta taatgacttc ctgttccggt
     3901 aacaaactcc gacagcttct tcagtgcttc tgcctcttct tcaatgaatc ccatatgtcc
     3961 ggaattttca agaacaaaaa cttcggcatt cggaggcagt ttcaccctgg tctgaatctg
     4021 cctgtaatca atatagttat ctccggcccc gagaatccac agtaagggaa cctttccccc
     4081 ttccatgacg gagagccggg aaggtctggc catcattccg ttgagaacgg aaataacagc
     4141 cttgtccgga attgttgaag cgatttccct tgaacgttca agttcgctgc tgaatttttc
     4201 cagactgccg gcagcataca tccttttgat attatctgcg aaaaaagtgt
//
LOCUS       DAOSPX010000289         4248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18766_length_4248_cov_4.152635, whole genome shotgun sequence.
ACCESSION   DAOSPX010000289 DAOSPX010000000
VERSION     DAOSPX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18766_length_4248_cov_4.152635"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PLZ75_11805"
     CDS             <1..1010
                     /locus_tag="PLZ75_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73118.1"
                     /translation="SWKWGNAGFVKDNIEWGNNYNGANIFGGRNPSFIQLRLQLRPAK
                     WFTFNYFHGWLNSMVVDSANSYWMSNSYGTNYREVYHRKFLTANMLTVTPFKYLNISA
                     GNSIVYNNTSVNPAYLMPLFFYKSVDHSVSSGIDNMNSQMFFDISSRQIRHLHLYATL
                     FVDELAISRISKKDAWNFLSWKSGFRLNNFPFSDTYFTTEFTYSYPLTYQHYVPTTTF
                     ESNNYNLGHYLKDNSREWFISAGYRPVGNLDIQLFFSDAVRGPDYTELAAPRVGNPPL
                     ESVEWRNTSAGLKAVYQLINDLYAQFSFSYSNISGNKNWSPEYLQGKKLTVNFGITFG
                     F"
     gene            1215..2900
                     /locus_tag="PLZ75_11810"
     CDS             1215..2900
                     /locus_tag="PLZ75_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOS73119.1"
                     /translation="MKRNKFRVITLFTDIVILAVSFMIAVLFRPAGISSYVPSHGVFF
                     IILAVIWLTVSIVNGKMHRGKIINFTSLFNRVIGSNLISVSLLALIMYIIRDYSYSRA
                     VFLGTTVIATIFELLAGSVYIAYKKANIRDYDEAEKFRKYRKPTEEELVNNLNGNGNG
                     FRNGNGNGNGNAYVYWNGNGNGNGNGHSHGNANGNGNGNGNGNGIDHENLQNVSPGII
                     EKIERKVGPEMTGAILAMTGPNLNGRTAVLSTTTVFNVENLPKEKYTYLINLHRINDI
                     AHLDSFIEAVNNKIELNGYFFCCVETKDQRKKRILQKYPAGLNYIYYSFDFILKRILP
                     KLKFTRPLYMFLTNGGNSVISRAEALGRLCRGGFHIMQESFIGNQLCIEAKKIRKPLP
                     VNGYSYGPIIALPRIGKDGKQIKVYKLRTMHPYSEYIQDYVYKMHNLQEGGKFKNDFR
                     ITTWGAFCRKVWLDELPMLINMFKGNMKLVGVRPLSKQYFNLYDDGVKEKRLKHKPGL
                     VPPYYADMPASLEEIQESEMRYLEEFEKQPVKTDLRYFFKSWWNIFFHEARSN"
     gene            3039..3542
                     /locus_tag="PLZ75_11815"
     CDS             3039..3542
                     /locus_tag="PLZ75_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73120.1"
                     /translation="MKKILLLIIASMFITFSRAQKCSDWIITEYDEVADITVISMKER
                     MYIEKDGQRKVSIYVARKIENNEKQTVLTIDVRDGGCINNLNGIIFLFTDGTRLKQMN
                     HGPYNCEGFAMLHIDIPDYEGHTIQDYLKQKKIKTLRVYTNDSFIDVDFSEANQVKLI
                     NTLKCIE"
     gene            3777..>4248
                     /locus_tag="PLZ75_11820"
     CDS             3777..>4248
                     /locus_tag="PLZ75_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOS73121.1"
                     /translation="MNKGRKKVFVSGCFDMLHSGHVAFLQEAAQYGDLYIGLGSDKTI
                     RELKGRETVYSEEERAYMLSSLKSVHRVSVNTGSGLMDFRDDMKNLAPDIFIVNEDGH
                     SPEKEELCTELGIEYRVLKRIPHANLPARSTTALRSSRPIPYRIDIAGTWIDQPY"
BASE COUNT         1334 a          881 c          925 g         1108 t
ORIGIN      
        1 attcatggaa atggggaaat gcaggatttg tgaaagacaa cattgaatgg ggaaataatt
       61 ataacggtgc gaatatcttc ggcggaagga atccttcatt catacagctg aggcttcagc
      121 tcagacctgc aaaatggttc acgttcaact atttccacgg atggctcaat tcaatggtgg
      181 tggacagcgc aaactcctac tggatgtcaa acagttacgg cacaaattac cgggaagtgt
      241 atcacaggaa atttcttaca gcaaacatgt taactgtcac tccgtttaaa tacctgaaca
      301 tctccgcggg aaacagcata gtatacaaca acacatcggt aaacccggca taccttatgc
      361 cgctgttctt ctacaagtct gtggaccatt cagtatcatc aggtattgac aacatgaatt
      421 cccagatgtt ttttgatata agttccaggc agatcagaca tcttcacctg tacgcgactc
      481 tctttgttga tgaacttgca ataagccgta tatccaaaaa ggatgcatgg aacttcctga
      541 gctggaaaag cggattccgg ctgaataatt ttcctttcag cgatacatat ttcactacgg
      601 aattcactta ttcctacccg cttacctatc agcattatgt gccgaccact acatttgaat
      661 caaacaatta caacctcggg cactatctta aggataattc aagggaatgg ttcatatctg
      721 ccggataccg gccggtgggc aatctggata ttcagctgtt tttctcagat gcagtcagag
      781 gaccggatta taccgaactt gctgccccca gggttggcaa ccctcccctt gaatcagttg
      841 aatggaggaa cacatctgcc ggactgaaag ctgtttatca gctgatcaac gatctatatg
      901 cgcaattttc gttttcttac agtaatattt ccgggaacaa gaactggagt ccggagtatt
      961 tacagggaaa gaaattaaca gttaattttg gaatcacatt cggattctag caaggccgga
     1021 tatacatata tatcagaaat cctgtgtgtt aatccgtatc tatttaaaac tggtctgata
     1081 aaaaatctgc cggaaaacat cacagcaaca gctgttcttc aagaccgttg aaaaattccc
     1141 tgaatccccg ctcgtttttt agctgaaaaa acttatattt ggcacgtcct ttatcaggga
     1201 cctactgtca aattgtgaaa agaaataaat tcagggtaat cacgctgttt accgatattg
     1261 ttattcttgc agtatcgttt atgatcgcgg tccttttcag gcccgccggg atcagttcct
     1321 atgtcccttc ccatggtgtt tttttcataa tcctggccgt gatctggctg actgtctcca
     1381 tagtcaacgg caaaatgcat aggggtaaga ttattaattt taccagcctc ttcaacaggg
     1441 taatagggag caacctgata tccgtgtccc ttctcgccct gataatgtat atcataaggg
     1501 attattccta ttcacgtgcc gtgttcctgg gaaccacagt aatagcaacc atcttcgaac
     1561 ttttggcggg atctgtgtat atagcctata aaaaggccaa tatccgcgat tatgatgaag
     1621 ctgaaaaatt caggaagtac aggaagccta cggaagagga gctggtaaac aatcttaacg
     1681 gaaatggcaa tggcttcagg aatggcaacg gaaacggaaa tggtaacgct tacgtttact
     1741 ggaacggtaa tggcaacggc aacggaaatg gccacagcca cggaaacgct aacggaaacg
     1801 gaaacggaaa cggaaatgga aacggcattg atcacgaaaa cttgcagaac gtcagtcccg
     1861 ggataataga aaaaatagaa aggaaagtgg gacctgaaat gaccggggca atactggcaa
     1921 tgaccggacc caacctgaat ggccgtaccg cggttttatc cacaaccaca gttttcaacg
     1981 tcgaaaatct ccctaaagaa aaatacacat acctaatcaa cctgcacagg ataaatgaca
     2041 tagcacacct cgacagcttt attgaggctg tcaataataa aattgaatta aacgggtact
     2101 tcttctgctg tgtcgagaca aaggatcaga ggaaaaaaag gatactgcag aaatatcctg
     2161 ccggtcttaa ttacatctat tattcctttg attttattct gaaacgcatt ttgccgaaac
     2221 taaaattcac ccgtcccctg tacatgtttc tgacaaacgg cggaaactcc gttatatcgc
     2281 gtgccgaggc ccttggccgc ctctgcaggg gaggatttca tatcatgcag gaatcattca
     2341 tcggaaacca gctctgcata gaggcaaaaa agataaggaa gccccttccg gtgaacggat
     2401 attcctatgg tccgatcatt gccctgccgc ggataggaaa ggacggaaag cagattaaag
     2461 tctacaagct caggaccatg cacccctatt cggaatatat ccaggattat gtttacaaaa
     2521 tgcataatct tcaggaagga ggcaagttca aaaatgattt ccggattaca acctgggggg
     2581 ccttctgcag aaaagtatgg ctcgacgagc tgccaatgct tatcaatatg ttcaagggca
     2641 acatgaagct tgtgggtgta agaccgctca gcaaacaata tttcaacctc tatgatgacg
     2701 gggtgaagga aaaaagactg aaacacaagc cgggtctggt acctccgtat tatgccgaca
     2761 tgcctgccag cctcgaggag atacaggaat ctgagatgag atatctcgaa gaatttgaaa
     2821 aacaaccggt aaaaacagat ttaagatatt ttttcaaatc gtggtggaat attttcttcc
     2881 acgaagccag aagcaactga ttcccccggt ccggtttatt gttgcaggtt cagaaaaagc
     2941 gaaaacagat tatcaaatct tttaaccgtt gtatcagagt atatatatct gttaaatact
     3001 caaaggtctg ttttaacctg aaactaaaga atctctgtat gaaaaaaata ttgcttctta
     3061 ttatagcttc aatgttcatt acattttcca gggcacaaaa atgctccgac tggattatta
     3121 ctgaatatga cgaagttgct gatataacag taatttccat gaaagaaagg atgtatattg
     3181 aaaaggatgg tcagcggaaa gtttcaatat atgtagcccg gaaaattgaa aataacgaga
     3241 aacaaactgt gcttacaata gatgtaagag atggtggatg tattaataat cttaatggta
     3301 taattttcct ttttacagat ggtacgaggt taaagcagat gaatcatggt ccttataatt
     3361 gtgaaggttt tgcaatgctg catatagata tcccggacta tgaaggacat acaatccagg
     3421 attacctgaa acagaaaaag attaaaacac ttcgcgttta cacaaatgat tcatttattg
     3481 acgttgattt ttcagaagca aatcaggtaa aattgataaa tacccttaaa tgtattgaat
     3541 aattgatggt gtcaggatga cttgcagcaa ctgctttttc agatggaaaa atcagtctga
     3601 aattccgggg ataatatact ctggcaaaat acccggaatc aataagatcc ctagcggaac
     3661 ggagattcac caatcaaaat tgttaacttt atgccggttt gccggctccg tccgtgctga
     3721 cgcagacagc agagccgggc aaataatcag tgactgaaac tattaccagg tataacatga
     3781 ataaaggaag gaaaaaagtt tttgtatccg gttgttttga tatgctccat tcggggcatg
     3841 ttgcttttct gcaggaagcc gcacaatacg gtgaccttta tattggtctg ggttcagata
     3901 aaaccatcag ggaactgaag ggcagggaaa ccgtctattc ggaagaggaa agggcctata
     3961 tgctgagctc cctgaaaagt gtgcacaggg tttccgttaa cacgggaagc ggactcatgg
     4021 atttccggga tgacatgaaa aatcttgctc ccgatatctt cattgtaaac gaggacggac
     4081 actcaccgga aaaggaagag ctctgcacgg aactgggaat tgaatacaga gtactgaaaa
     4141 ggattccgca tgcaaacctt ccggcgcggt caacaacggc attgcgttcc tcccggccca
     4201 tcccctacag gattgacatt gccggaacat ggatagatca gccatatg
//
LOCUS       DAOSPX010000290         4244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18791_length_4244_cov_4.495345, whole genome shotgun sequence.
ACCESSION   DAOSPX010000290 DAOSPX010000000
VERSION     DAOSPX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18791_length_4244_cov_4.495345"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLZ75_11825"
     CDS             <1..469
                     /locus_tag="PLZ75_11825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS73122.1"
                     /translation="IPAILPISNKDLTRTASGFGNRVHPIYKIIKFHSGMDFTAPTGT
                     EIYATGNGRIQSVTSTKRGLGNHIIIDHGFGYTTVYAHLDRFNVRQGQKVSRGDVIGY
                     VGNTGLSVAPHLHYEVKLNGVNVDPVNYYFNDLTPEEYDRMIEIASKTGQSFD"
     gene            489..1646
                     /locus_tag="PLZ75_11830"
     CDS             489..1646
                     /locus_tag="PLZ75_11830"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOS73123.1"
                     /translation="MKNNRNLVLLCFALSLIICLTANSQIPDTLDFPAGQASDSVPAT
                     MQDQDHLLTADTVSQKQRSLVITGVGDIMFGTTYPSRRFLPRHDDPLVLLGDLADSLA
                     MSDITFGNLEGSFLDKGDPAKKCRDTTICYLFRMPERYAGALRVAGFDILSLANNHFG
                     DFGQASRNRTMELLDSLEIEYAGLIEKPWAIFARDSVVYGFCAFAPNAGTVSITDIPS
                     AERLVRFLSDTCDIVIVSFHGGAEGAEHQHVTRKEEISFGENRGNVYEFSHRLIDAGA
                     DIVFGHGPHVTRAVEVYRERFIGYSLGNFCTYGRFNLSGPNGIAPVIKVNVDMTGRFL
                     SARIIPVFQVYNGGVRVDPDKRVIRKIRELTAMDFPESVIDISDDGEIKYK"
     gene            1649..1984
                     /locus_tag="PLZ75_11835"
     CDS             1649..1984
                     /locus_tag="PLZ75_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOS73124.1"
                     /translation="MPYKERKVEKLYYSIGEVSEMLEVPVSTVRYWENEFDILKPMKN
                     KKGNRMFTASDIKNLKMIHHLLKVEGMTLQGVKRKLGGKREETESSYEITESLMRIRD
                     LLTELKNNM"
     gene            2157..3275
                     /locus_tag="PLZ75_11840"
     CDS             2157..3275
                     /locus_tag="PLZ75_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOS73125.1"
                     /translation="MKLKEIADFLDSAIPLSFQEDYDNSGLQCGLPEMEISSALLTLD
                     VTEEVVGEAVVTGCNMIISHHPVIFRPLKRISESTAAEKIIARAIRQDIAIYSAHTNL
                     DIIENGVSRKMAEKLDLSDIRVLVPLKDRLLKLVVFVPDDHLGRVSQAVFEAGAGIMG
                     NYDSCGFTVSGTGSFRAGEDADPFVGEKGRLHFEKESRFETIFYSHLRPGVIKALLKA
                     HPYEEVAYDIYPLENQNAGAGLGCTGNLTEPLEEKEFLRLLSSVFGAEFIRHSVLRGR
                     KIRKAALCGGSGGSLADAAAASGADAFITADIKYHTFLDSGSRMLLADIGHYESEKFS
                     IEILHEIIIKKFPKFALRFSEINTNPINYLQYGKSQNA"
     gene            3253..4017
                     /locus_tag="PLZ75_11845"
     CDS             3253..4017
                     /locus_tag="PLZ75_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOS73126.1"
                     /translation="MEKVRTHDTEISVEDRLRALSSLQLVDSEIDKIKTLRGELPLEV
                     QDLEDEIAGLETRLGNLREEVVMLEKSVQKKNIEISEAESLIKKYEDQQRNVRNNREF
                     DSLSKEIEFQNLEIELFNKKIREFNAQADEKRKLISESEAALAERQGDLENKRSELDE
                     IISDTQKEEEILLKKHEEVQDVIEERLLAAYRRIRANARNGLAVVPVQRDACGGCFNQ
                     IPPQRQLDIKSRKKIIVCEYCGRILVDDEIIEQSKE"
BASE COUNT         1239 a          819 c         1077 g         1109 t
ORIGIN      
        1 cattccggca atcctcccga taagcaataa ggacctgacc aggacagcat cgggtttcgg
       61 caacagggtc catcccatct acaaaataat caagttccat tcaggtatgg atttcaccgc
      121 accaacagga actgaaatat atgccacggg taacggcagg atacagtcgg tcacttcaac
      181 caagagaggc ctcggaaacc atatcatcat cgatcatggt ttcggatata cgacagtcta
      241 tgctcatctt gaccgattca acgtccgtca gggtcagaaa gtatccaggg gcgatgtaat
      301 aggatatgtt ggcaacacag gtctgtctgt ggcaccccat ctgcattacg aggtgaagct
      361 taacggggtg aatgttgatc cggtcaatta ctatttcaat gatctcactc ccgaggaata
      421 cgatcgcatg atagaaatag cttccaaaac gggccagtcc tttgattaag ttcattttaa
      481 attttccgat gaagaataac agaaacctgg tccttttatg ttttgcgctt tcacttatca
      541 tttgcctcac agctaattcc cagataccgg atacacttga ttttcctgcc gggcaagcca
      601 gtgattccgt tccggccacg atgcaggatc aggaccattt gcttacggca gatactgttt
      661 cacagaaaca aagaagcctg gttattaccg gggtgggaga cataatgttc ggcaccactt
      721 atccgtccag gcgctttctg ccccggcatg atgatcccct ggtacttctc ggggatcttg
      781 cggactcact tgcgatgtct gacatcacat tcggcaatct tgagggatca tttctggaca
      841 aaggtgatcc cgccaaaaag tgcagggata caaccatttg ctatcttttc agaatgccgg
      901 aaagatatgc cggggcactg cgggttgccg gttttgatat tctgagcctt gcaaacaatc
      961 acttcggtga ttttggtcag gcttcccgca ataggaccat ggaactgctt gattcgctcg
     1021 aaatagaata tgcaggactc attgaaaagc catgggcgat attcgccagg gattctgttg
     1081 tttacgggtt ctgtgccttt gcaccaaatg caggcactgt cagtattacc gatattccct
     1141 cggctgagag gctggtaaga tttctaagtg atacctgcga tatcgttatt gtctcttttc
     1201 acggaggggc tgaaggggcc gaacatcagc atgtgacccg gaaggaagag atttcattcg
     1261 gtgaaaacag gggcaatgtg tatgaatttt cccacagact gatcgatgcc ggggcagata
     1321 tagttttcgg tcacgggcct catgtgacac gggccgtgga agtgtacagg gaaagattta
     1381 taggctacag cctgggaaat ttctgcacct acggcagatt taacctgtca gggccaaacg
     1441 ggatagctcc ggtgataaag gtgaatgttg atatgacggg gagatttctt tccgcaagaa
     1501 taattccggt atttcaggta tataacgggg gtgtgagagt ggatcctgac aaaagggtga
     1561 taaggaaaat cagggagctg acagccatgg actttcccga atctgtgatc gatatttcgg
     1621 atgatggtga aataaagtat aaatagccat gccatataag gaaagaaagg ttgaaaagct
     1681 gtattactcc ataggagaag tgtcggagat gcttgaggtg ccggtgtcca ccgtcaggta
     1741 ctgggaaaat gaattcgata ttctgaagcc catgaaaaat aagaagggca acaggatgtt
     1801 cacagcttct gacattaaaa atctgaaaat gattcatcat ttgctcaagg tggaaggaat
     1861 gacgcttcag ggcgtgaaaa gaaaactggg cggcaaacgg gaagaaacgg aatccagcta
     1921 tgagatcacg gaatcactca tgagaatcag ggatctgctg actgaattaa agaataatat
     1981 gtagttgtcc ggagagtttt caggaggttt tgctttaaga tattttgttt tccgttattt
     2041 tatatttcca gcatcttttt tgtgtatatt cctgcgattt aactgtttta ttgtaattca
     2101 aaggagacaa gtctgctttc aggcaaaaga agaattcaaa aaagtaagaa cagggtatga
     2161 aattaaaaga aattgctgat tttctggatt ctgctattcc gctttcattt caggaagact
     2221 atgacaattc gggattgcag tgtggattgc cggaaatgga aatcagttct gcattgctca
     2281 ctcttgatgt gacggaggaa gttgtagggg aagctgtcgt taccgggtgc aacatgatca
     2341 tttcacatca tcctgtaatt ttcaggcccc tgaaaaggat ttcggaaagt acagctgcag
     2401 aaaagataat agccagggcc ataaggcagg atattgccat ttattctgct catacgaatc
     2461 ttgacattat tgaaaacggg gtcagcagaa aaatggctga aaagctggat ctctcggata
     2521 taagggtatt ggtgccgctg aaggaccgtc tgctgaaact tgttgtcttt gttcctgatg
     2581 atcaccttgg cagggtgagt caggcagtat ttgaagccgg ggcaggaata atgggaaatt
     2641 atgacagctg tggttttacc gtcagcggta ccggcagttt cagggcgggt gaggatgcag
     2701 atccttttgt gggggagaaa ggcaggctgc atttcgaaaa ggaaagcagg ttcgagacaa
     2761 tattttattc acatctcagg ccgggggtga taaaagcgct tctgaaagca catccgtacg
     2821 aggaggtggc atatgatatc tatcccctgg aaaatcagaa tgccggagcc gggctgggct
     2881 gtaccgggaa tctgacagaa ccattggaag aaaaggagtt tttaaggctg ctatcctctg
     2941 ttttcggtgc tgaatttata aggcattcgg tgctgagggg caggaagatc agaaaagctg
     3001 ccctttgcgg aggttcagga ggctcccttg ctgatgctgc tgccgcttcc ggggcagatg
     3061 cctttattac cgcggatatt aaatatcata cttttcttga ttcgggcagc agaatgcttt
     3121 tagccgatat cggacattat gaaagcgaaa aattttcaat tgagatttta cacgaaataa
     3181 ttatcaaaaa attccctaaa tttgcactcc ggttttcaga aataaatacg aatccgataa
     3241 attatttaca atatggaaaa agtcagaacg catgatacgg agatttcggt ggaggacagg
     3301 ctgcgtgctt taagcagtct gcagcttgta gattcagaaa ttgacaagat aaaaacactg
     3361 agaggcgaac ttccccttga agtgcaggat ctggaagatg agatagcggg tctggaaaca
     3421 agacttggca atctgagaga ggaagttgtg atgcttgaaa aatcggtaca gaaaaaaaat
     3481 attgaaataa gtgaagctga gtcgcttata aagaaatacg aggatcagca gagaaatgtc
     3541 agaaataaca gggagtttga ttcactttcc aaggaaatag aatttcagaa tctcgaaatt
     3601 gagctgttca acaaaaagat cagagagttc aatgcccagg cagacgaaaa acggaagctg
     3661 atttctgaat cagaggctgc actggccgag cggcagggtg acctggagaa caagcgctcg
     3721 gagcttgatg agatcatttc cgatacacag aaggaggagg agattcttct gaaaaagcat
     3781 gaggaagtac aggatgttat cgaagagcgt ttgcttgcag catacaggag aatacgcgca
     3841 aatgcaagga acgggctggc agttgtaccc gttcagcgtg atgcctgcgg cggttgtttt
     3901 aaccagatac ctcctcagcg acagcttgat atcaagtcga gaaagaaaat cattgtctgt
     3961 gaatattgcg gccgtatact tgtggatgat gaaataatag aacaaagcaa ggagtaatta
     4021 caggaatata aattgaatgc cgtccggatc acatctcagg acggcatttt ttttgccgtt
     4081 ttgcagaaat gctttttatc ctgaattcat taactttact ctgtaggttc acaggatttt
     4141 aatgagtccc tgtgtcagca gaaatatcca tatacattta ttctgataaa atcagcatct
     4201 catgtcagaa acaattttcc agaaaagcat agctgagggc attc
//
LOCUS       DAOSPX010000291         4225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18923_length_4225_cov_3.833333, whole genome shotgun sequence.
ACCESSION   DAOSPX010000291 DAOSPX010000000
VERSION     DAOSPX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18923_length_4225_cov_3.833333"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1458)
                     /locus_tag="PLZ75_11850"
     CDS             complement(247..1458)
                     /locus_tag="PLZ75_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOS73127.1"
                     /translation="MKLSRIPLLGRVGIAIVLGIITGQFAPVWFARIFATFNSLFGNF
                     LSFIIPLIIVGLITPAIGELGKGAGRLLAITALIAYLSTLFSGFFTYFSCQAVYPKIL
                     TGGIDAMMLDTIDEGLVEPFFTVAMPPVMEVMTALILSFCVGLGITFLKGDTLQNAFQ
                     ELREIITLIIRAIIIPFLPLYIFGMFLAISISGQVYSVIMLFLKVILVIFILHILLLI
                     IQFLVAGVITGRNPFKALRTMLPAYFTALGTSSSAATIPVTLECTIKNRVNPSIASFT
                     IPLCATIHLAGSTMKIVGFALALQYFFGIPVDFATFIGFIFMLGIIMIAAPGVPGGAI
                     MAAIAVIQSILGFSEQMIGMMITLYIAVDSFGTACNVTGDGAIALVMDHISGKEKNAV
                     KADVAGEGSIA"
     gene            complement(1598..1670)
                     /locus_tag="PLZ75_11855"
     tRNA            complement(1598..1670)
                     /locus_tag="PLZ75_11855"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1635..1637),aa:Gln,seq:ttg)
     gene            complement(1756..2181)
                     /locus_tag="PLZ75_11860"
     CDS             complement(1756..2181)
                     /locus_tag="PLZ75_11860"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HOS73128.1"
                     /translation="MSYPAKLSIEPTRRSPWVVLEPGRIFVMGRSIIENPSHFYTPGY
                     EWIMEFIKVWSGSIKVDLGFEYINTGSIKWLYILLKRLSEEERVAENAEITWYYEHGD
                     EDMRELGSILRSLINCRFRFVETDAMNDAFYNRFFTEKN"
     gene            complement(2546..3517)
                     /locus_tag="PLZ75_11865"
     CDS             complement(2546..3517)
                     /locus_tag="PLZ75_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOS73129.1"
                     /translation="MLHYKELGLVNSRELFRDAMKGGYAVPAFNFNNLEQLQAIISAC
                     VETKSPVILQVSKGARKYANQTLLQYLAQGAVAYAKELGHEIPIVLHLDHGDTFETCK
                     SCIETGFSSVMIDGSHFPYEENVRLTREVVEYAHKFDVTVEGELGVLAGIEDEVSSEH
                     STYTRPEEVEDFVKKTGVDSLAISIGTSHGAFKFKVKPGELPPPLRFDILEECEKRIP
                     GFPIVLHGASSVPQDIVAEINKYGGKLENTAGVSEDQLRRAARSAVCKINIDSDGRLA
                     MTAAVRKVLTEKPGEFDPRNYLGPARDKLKELYKHKIINVLGSADRV"
     gene            3758..>4225
                     /locus_tag="PLZ75_11870"
     CDS             3758..>4225
                     /locus_tag="PLZ75_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS73130.1"
                     /translation="MKKILILLIVLFSLPPGQVLPQEKIWTLEECIQYAIDNNIQIKQ
                     QVLQTRFQENTLNQSKLKLLPSINGQAAHNFSFNRTLDQTTYQYVDQNGQSNQFYLGG
                     NMDIFNGLQNYNTIRKNKYQLISSELDLESLKNNISLNIALAFLQILLNSDLVN"
BASE COUNT         1283 a          976 c          878 g         1088 t
ORIGIN      
        1 accggaggtc gattaggatg ctttccgctt ttcactctat gttgataaca ccaacagcaa
       61 gggcagtgat gaaaatcccg atacagagga gctccccctg cagaccatac agcatgccgc
      121 caatatagcc ggtcccggtg atgtgatatg tgtaaaaaac ggcagctatc gtgaacgggt
      181 gattgtcaaa aagggatgag aggccggcaa accggttaca attccttttc aggcaagact
      241 tccacctcag gcaatactcc cttctccggc aacatctgct tttacagcat tcttctcctt
      301 tcctgaaata tggtccatta ccagtgctat ggcaccgtca cctgtgacgt tacaggcggt
      361 tccgaagcta tcaacggcaa tgtagagggt gatcatcatc ccgatcattt gttcactgaa
      421 gcccaggatg ctctgaataa ccgcaattgc tgccatgata gcgcctcccg gaaccccggg
      481 agctgcaatc attattatgc caagcatgaa tatgaaaccg atgaaagtgg caaaatcgac
      541 cggtatgccg aaaaagtact ggagagccag tgcaaaaccc actatcttca tcgtgcttcc
      601 tgccagatga attgttgcac agagcggtat ggtaaatgag gcaatactgg gattgaccct
      661 gttttttatg gtacattcaa gtgtaaccgg tatggtagca gcagaagacg aggtgcccag
      721 ggcagtgaaa taggccggaa gcatggttct cagagccttg aaaggattcc tgcccgtaat
      781 tactccggcc acgaggaact gaatgatcag cagaaggata tgaagaataa aaattaccag
      841 tataaccttc aggaaaagca ttattaccga atagacttga cccgagatgg aaatggcgag
      901 gaacattccg aaaatgtaga gcggaagaaa cgggataata atagccctga tgatcagggt
      961 gataatctcc cgtaattcct gaaatgcatt ctgtagggta tctcccttaa ggaatgttat
     1021 acccagcccg acacagaatg agagtatcag ggcggtcatc acctccatca ccggaggcat
     1081 cgccacagtg aagaagggtt caacaagccc ctcatcaata gtatcaagca tcatggcatc
     1141 aattccgccg gtgagtatct tcggatatac tgcctgacat gaaaaatagg taaagaagcc
     1201 cgaaaagagg gttgacaggt atgctatcag ggcagtgatc gccagcagcc tgccggcacc
     1261 ctttccaagc tcacctatgg caggtgtgat gagaccaaca attataagcg ggatgatgaa
     1321 cgacaggaaa ttgccaaaaa gtgagttgaa ggtggcgaag atgcgtgcaa accataccgg
     1381 ggcaaactgt ccggtaataa tgcccagaac gatggcaatc ccgactcttc ccagcagggg
     1441 tatccttgaa agcttcatag caggctgata aaatatgtgc gctaaagcta ctatttttta
     1501 aatatctccc accagagaaa aatcaggttt gtaaggaaag ggcggataaa aaaaccctga
     1561 taagaatgat aatcagggtt atggacataa agatttgttg acccacctgg gctcgaacca
     1621 ggactctgcg gaaccaaaat ccgatgtgtt accagttaca ccatgggtca gttttcctga
     1681 agaaccctgc aaaactataa aaaaaattga ttttattaat aacaaagaga taatcctttg
     1741 tctaagaatg ggatattaat tcttttcagt aaagaaccgg ttgtaaaaag catcattcat
     1801 ggcgtcagtc tccacaaatc tgaaacggca gttaataagt gaacggagta ttgatccgag
     1861 ttcccgcata tcttcatcac cgtgttcata ataccacgtt atctcagcat tttcagcaac
     1921 cctctcttct tcagacaatc tctttaaaag aatataaagc cattttatgg atcctgtgtt
     1981 tatgtattca aagccaagat caacttttat actccccgac cataccttga taaattccat
     2041 tatccattca tatccgggcg tatagaaatg tgaagggttt tcaatgatgg acctccccat
     2101 cacaaaaatg cgtccgggct ccagtaccac ccagggagat cttcttgtag gttctataga
     2161 taatttagca ggatatgaca tactgcagat ttacttattt taaacatgac cttcacaaac
     2221 catattacgc tgtaatacgg aatcagttac agtaatgtgc aaaaatacaa aagattcacg
     2281 tcttaacaaa atatcaggta aattcctccg tgaatacccg ggatccaaag aggaaaaccg
     2341 gaataatata ccgggagatt cacctttact cagggaaaga aaaacccttg ccttgcctgc
     2401 ctgtcagcta agctgcagaa gggaagagcc ccgcttaact ataaaaaagg tagtatgcca
     2461 acgtatcaat aaaccaacac aaacaaagga ttcactttta aatgccggaa aagaaagacc
     2521 agagcatcat gaaaaactca tcctgtcata ctctgtcggc acttcccagt acgttaatta
     2581 tcttatgctt gtacagttcc ttaagcttgt cgcgtgccgg tccgagataa ttgcggggat
     2641 cgaactctcc gggtttctcg gtcagcacct tgcgtactgc ggctgtcatg gcaagccgtc
     2701 cgtcactgtc gatatttatt ttgcagacag ccgaccttgc tgccctgcga agctgatcct
     2761 cggatacacc tgctgtgttc tcaagctttc cgccgtattt gttgatctcg gctacaatgt
     2821 cctgaggtac tgaagaggca ccgtgcaata caatggggaa cccgggaata cgcttttcac
     2881 attcctcgag aatatcaaag cgaagggggg gaggaagttc tcccggttta accttgaatt
     2941 taaatgctcc gtgtgaagtg cctattgata ttgccaggga atcaaccccg gtctttttca
     3001 caaaatcctc aacctcctca ggtcttgtat aagtagaatg ttctgatgaa acttcatctt
     3061 ctatgcctgc cagaaccccc agttcaccct caacggttac atcaaactta tgggcatact
     3121 caacaacctc tcttgtcagc cttacattct cctcgtaggg gaaatgcgat ccgtcaatca
     3181 ttaccgaaga aaatccggtt tctatacatg atttgcaggt ctcgaatgta tcaccatggt
     3241 cgaggtgcag cactattggt atttcatgac caagttcctt tgcataagcc accgcaccct
     3301 gtgccagata ttgcagaagt gtctgattgg catatttgcg tgctcctttt gaaacctgaa
     3361 gaataaccgg agatttagtc tccacgcagg cactgatgat agcctgcaac tgctcgagat
     3421 tattgaaatt gaatgcaggg acagcatacc cacccttcat ggcatccctg aacaattccc
     3481 tcgagttaac caatcctaat tccttataat gtaacatatt tttaagtgtt tgtaaatagg
     3541 cgtgacaaga tagtcatttt ttaaattctg tatcaaaaaa taatgatatc caaccatctt
     3601 ttaaatatga caggtaagat taattgatca gaatcattta tcttcgtagt cctgaaaagg
     3661 ccgcaattgc cttaacttat tctgttcccc gggatgcagc aaatacaata atttgatcat
     3721 taggattcgt tgcggacaaa acaaacaagc tgttcagatg aaaaagatat tgattttatt
     3781 gattgtcctt ttttcacttc ctccgggaca agtccttccc caggaaaaaa tatggacact
     3841 tgaggaatgc atacaatatg cgatcgacaa taatattcag ataaagcaac aggtgcttca
     3901 gaccaggttt caggaaaaca cccttaacca gtcaaagctt aaactgctgc cttccatcaa
     3961 tggccaggca gcccataatt tttcattcaa ccgtaccctc gaccagacaa cctatcagta
     4021 tgttgatcag aacggccagt cgaatcagtt ctatctggga gggaacatgg atattttcaa
     4081 tggtcttcag aattacaaca ccataagaaa gaacaaatac cagctaattt caagtgaact
     4141 tgaccttgaa agcctgaaaa acaatatatc cctgaatatt gccctggctt ttctccagat
     4201 cctccttaac agtgatcttg taaac
//
LOCUS       DAOSPX010000292         4219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_18958_length_4219_cov_4.101105, whole genome shotgun sequence.
ACCESSION   DAOSPX010000292 DAOSPX010000000
VERSION     DAOSPX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18958_length_4219_cov_4.101105"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLZ75_11875"
     CDS             complement(<1..288)
                     /locus_tag="PLZ75_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS73131.1"
                     /translation="MEGSLLKGKKGLIFGALNEKSIAWQVALKTWQHGARLVLTNSPV
                     AVRMGSIDELARITGSSVIPADATRMDDIEKLISETMEIFGGRFDFILHSIG"
     gene            complement(299..1963)
                     /gene="recN"
                     /locus_tag="PLZ75_11880"
     CDS             complement(299..1963)
                     /gene="recN"
                     /locus_tag="PLZ75_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOS73132.1"
                     /translation="MLVRLSVRNYALIRDLDIDFGNGLTIITGETGAGKTILLGALSL
                     ILGARADTSVLLDKENKCIVEGTFSVEGHEMKSFFEENDLDYDSHAILRREISSAGKS
                     RAFINDTPVNITILKELGESLIDIHSQHQTLMLNNNSFQLNVVDSFAGTAAVLDEYRE
                     RYIKYRNLRRAYAGESAKAEKNRADLEYYRFQLNQLDSARLTEGEQEELEREQELLSH
                     AEEIVTGLAAITECFSAESGSILSSLSELRNILNRIKGFLPGGEELAARTESSYIELN
                     DLSSEIEGLSASISADPARLAQVNERLDTIYTLARKHQVNGLDELLVKREEIRKLVQS
                     IETGDERLAEMESLIRSEEAVLTKMASSITGKRKKSLPETEKNITGMLRQLGMPNARF
                     RIDFSSQADFSESGRDQVDFLFSANKQIDPENLAKTASGGELSRVMLSLKSFLTRNVS
                     LPTIIFDEIDSGVSGEVADKVGQILSSMGKYMQVINITHLPQVASRGARHFHVYKEDE
                     GDSTITRVKLLSGDERIMEVARLLSGSEVTQAALKNARELLEAAGN"
     gene            complement(1975..2901)
                     /locus_tag="PLZ75_11885"
     CDS             complement(1975..2901)
                     /locus_tag="PLZ75_11885"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOS73133.1"
                     /translation="MRIIGSSGFIALMLLVLPAIRANSQELNCNVQVSAQKVQGSNRQ
                     VFENMQRDLYEFMNNIVWTNHVYSYAERIDCNLLITINEQISADEFNGTLQIQLRRPV
                     YNTTYNSTMLNFMDNNFRFRYVEFQPLEFDPNTHRSSLVSVLAFYAYIILGFDYDSFS
                     LKGGTDYFRIAEKIVTNAQNAPEPGWKPYDGSRNRNRYWLVKNLLDAQYDGARRFLYE
                     YNINGLDRLESRASEARVSIVESLRLLQEVYRNRPDPFMYLLQIIIDAKSEELINIFS
                     ESFPEEKSRVVQILNEIDPANKSRYEKITASN"
     gene            complement(3166..3498)
                     /locus_tag="PLZ75_11890"
     CDS             complement(3166..3498)
                     /locus_tag="PLZ75_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOS73134.1"
                     /translation="MDYRKSLAPPTTITRDIDNLTRQTGNIYETVIIVARRANQIAVE
                     MKQELNKKLEEFASYSDNLEEIFENREQIEISKFYERLPKPSLIALQELEEGKIFFRN
                     PEKTSRQK"
     gene            complement(3502..>4219)
                     /gene="bamD"
                     /locus_tag="PLZ75_11895"
     CDS             complement(3502..>4219)
                     /gene="bamD"
                     /locus_tag="PLZ75_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOS73135.1"
                     /translation="SKAKWYYENGQYVRATELLAQVLPRFRATEEAEDLNWMNAQSFY
                     KMKDYFAAGSYFKSYLDQYAYGKYAEDAAFMAAMCDYNLSPRAELDQESTRAAIEGFN
                     YFLNKYPYSARADECRNLIRELQERLVEKSYLSARLYYDMKAYKSAIVALNNSLKEYA
                     DTKYREEMMYLKLNSLFLYAEYSMPDKQKERYQDTLDDYYSFMEEFPKSKYSRDVRRI
                     FQATARFLKIDTTDLEANIQ"
BASE COUNT         1012 a         1032 c          850 g         1325 t
ORIGIN      
        1 tccgattgaa tgcaggatga aatcaaagcg gcctccgaat atctccatgg tttcggagat
       61 aagtttttca atatcatcca tcctggtggc atcagcgggg ataacactgc tgccggttat
      121 ccgggcaagt tcatcaatac tgcccattct taccgccacc ggcgagttgg taagcaccag
      181 ccgtgctccg tgctgccatg tcttcagagc cacctgccat gcaatcgact tttcattaag
      241 cgctccgaat atcaatcctt ttttgccttt taacaatgat ccttccatag tttttagttt
      301 aatttccggc tgcctcaagc agttctctcg cgtttttcag ggctgcctgg gttacctcgc
      361 ttccgctgag caatctggca acttccatta tcctttcatc tcctgataac aatttaaccc
      421 tggtaattgt tgaatcgcct tcgtcttcct tatagacatg aaaatgcctg gccccgcgtg
      481 atgccacctg gggaagatgt gtaatattga tcacctgcat atatttcccc atactgctga
      541 gtatttgtcc gaccttgtcc gcaacctctc ccgaaacccc tgaatcaatc tcatcaaaaa
      601 taattgtagg gaggctgaca ttcctggtaa gaaaggactt gagactgagc ataactcttg
      661 acaattcgcc tcccgaagca gtttttgcaa gattttccgg atcaatctgt ttgtttgctg
      721 aaaaaagaaa atcaacctga tcccttcccg attcactgaa atccgcctgt gacgaaaaat
      781 ctattctgaa tctcgcattt ggcattccga gctgcctgag cattccggta atattctttt
      841 ctgtttcagg cagggatttt ttccttttcc cggtgattga tgaagccatt tttgtcagga
      901 ccgcttcttc cgagcgtatc agcgattcca tttctgccag cctttcatca ccggtctcga
      961 ttgactgcac cagctttctt atctcttcgc gtttgacaag aagttcgtcc aatccgttta
     1021 cctgatgctt tctggcaagg gtgtaaatgg tgtctagcct ttcatttacc tgtgccagcc
     1081 tggcaggatc cgcacttatt gatgctgaca gcccctcgat ttccgaagaa aggtcattca
     1141 gctcaatgta cgacgattct gttcttgctg caagctcctc cccgccggga agaaaaccct
     1201 ttattctgtt cagtatattc cgcaattcac tcagactgct cagtatggaa cctgattcgg
     1261 ctgaaaaaca ctctgttatg gctgccagtc ctgtcacgat ttcctcagca tgcgaaagaa
     1321 gctcctgctc cctttccagc tcctcctgtt caccctccgt gagtctggca gaatcaagct
     1381 ggttcagctg aaacctgtaa tattccagat cagccctgtt tttttccgcc ttagcggatt
     1441 ccccggcata tgcacgtctg agattcctgt atttaatata tctttccctg tattcatcca
     1501 gaactgctgc cgtaccggca aaggaatcaa caacattgag ctgaaatgaa ttgttgttca
     1561 gcatcagtgt ctgatgctgt gaatgaatgt ctatcaggct ctctcccagt tcctttaaaa
     1621 tggtgatatt tacaggtgta tcattgatga atgcccgtga tttaccagca gagctaattt
     1681 ctctcctcag gattgcgtgg ctgtcataat caagatcgtt ttcctcaaaa aatgatttca
     1741 tttcatgacc ctcaacgctg aaagtaccct ctacaataca tttgttttct ttgtcgagaa
     1801 ggacggaagt atctgccctt gccccgagaa taagggacaa ggcacccagc aggatggttt
     1861 tacctgcccc tgtttcccct gtgattatag taaggccgtt tccgaaatca atatccagat
     1921 ccctgataag cgcatagttc cggacgctga gtctgactag catttggaat attgttaatt
     1981 tgaagctgtt attttttcgt acctggattt gttggccgga tctatttcgt tgagaatctg
     2041 cacaacacga ctcttttctt caggaaatga ttccgaaaag atattaatca gctcttcact
     2101 ctttgcatct ataataatct gaagaagata catgaaagga tccggcctgt tcctgtaaac
     2161 ttcctgcagt aacctgagac tctcaacaat gctcaccctg gcttccgagg cacgtgactc
     2221 gagcctgtca agtccgttga tattatattc gtaaagaaat cttctcgctc cgtcatactg
     2281 ggcatccagg agattcttga caagccagta cctgttacgg ttccgggaac cgtcataggg
     2341 tttccatccc ggttcgggag cattctgggc attggtgact attttttctg caatcctgaa
     2401 ataatcagtg cctcccttaa gtgaaaatga atcataatcg aagccgagta taatataggc
     2461 ataaaaggca agaacagaca ccaggctgga cctgtgagta ttggggtcga actcaagcgg
     2521 ctgaaattcc acatatctga acctgaaatt attgtccata aaattcagca tcgtcgaatt
     2581 atatgtggta ttatatacgg gacgccttaa ctgaatctgc agggtcccgt taaattcatc
     2641 ggcagatatc tgttcattaa tggttatcaa cagattgcag tcgatccgtt cggcatagct
     2701 gtatacatga tttgtccaga ctatgttatt catgaattca tacaggtcgc gctgcatgtt
     2761 ttcaaacacc tgacggttcg atccctgtac cttttgtgct gatacctgga cattgcagtt
     2821 cagttcctgt gaattcgccc gtattgccgg caatacaaga agcatcaggg caataaaacc
     2881 ggaagatcct atgattctca tctttttgtg atcattgtaa caattaatca tgaatgtctg
     2941 agacagtttc ccttttcgat tttaacccaa atttattaat attattgtac ttttcaatga
     3001 ttgttgcccg attcgtttta aaggcggaat atacagccgg ttcctgcata agaccatacc
     3061 tcccgggatt ttgcaatatc tgtgaagcca catcatccgg tgcatccggg gaattctgcc
     3121 cgggcggctt tcattgcccg ccagctttga aaggccggct gcctgttatt tctgtcttga
     3181 tgttttttcg gggttcctga aaaatatctt tccttcctca agttcctgaa gggcgatcag
     3241 gcttggcttc ggaaggcgct cataaaattt cgatatttct atctgctccc ggttttcgaa
     3301 aatttcctcg agattatcgc tgtatgatgc aaattcctcc agtttcttat tgagttcctg
     3361 cttcatttca actgcaatct gatttgccct gcgggcgaca ataatcactg tctcgtaaat
     3421 attgcctgtt tgcctggtca gattgtcaat atcgcgtgta attgttgttg gcggtgccaa
     3481 tgattttctg taatccatat tttattgaat atttgcttcc agatcagttg tatcaatttt
     3541 caggaacctt gcagttgcct gaaatatcct tctgacatcc cttgaatact tgcttttcgg
     3601 aaattcttcc ataaacgaat agtaatcatc aagtgtatcc tgatatctct ccttttgctt
     3661 gtcaggcata ctgtattcgg catagagaaa caatgaattg agtttgagat acatcatttc
     3721 ctcccggtat ttcgtgtcgg catattcctt gagactgttg ttcagggcca caattgccga
     3781 cttatacgct ttcatatcat agtaaagcct tgcactcaga tatgactttt ccaccagccg
     3841 ttcctggagt tcccttatga gatttctgca ttcatcagct cttgcactgt agggatactt
     3901 attgaggaaa tagttgaagc cctcaattgc ggcacgggtg ctttcctgat caagctctgc
     3961 cctgggcgac aggttataat cgcacatggc agccataaat gctgcatctt ctgcatattt
     4021 cccgtatgca tactggtcga ggtaggattt gaaataactt ccggcagcaa aataatcctt
     4081 catcttgtaa aagctttggg cattcatcca gttcaggtct tctgcctcct ctgtggccct
     4141 gaacctggga aggacctgtg ccagcaactc cgttgccctg acatactggc cattttcata
     4201 ataccatttt gccttcgac
//
LOCUS       DAOSPX010000293         4196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19082_length_4196_cov_4.954359, whole genome shotgun sequence.
ACCESSION   DAOSPX010000293 DAOSPX010000000
VERSION     DAOSPX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19082_length_4196_cov_4.954359"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2945)
                     /locus_tag="PLZ75_11900"
     CDS             complement(<1..2945)
                     /locus_tag="PLZ75_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HOS73136.1"
                     /translation="MSWIKDMISPQTREWEEFYRNRWQYDKVVRSTHGVNCTGGCGWN
                     VHVKEGIVVWELQALDYPRLENSLPPYEPRGCQRGISFSWYLYSPVRVKYPMIRGALL
                     DLFNEERKKTGDPLLAWQNIQNDPEKRRKYQKARGKGGFRRVAWTEVMELIGAANIYT
                     AKKYGADRVVGFSPIPAMSMISFAAGSRFLQLFGGVHLSFYDWYCDLPSSFPEVWGEQ
                     TDVGESADWYNARFIAVMGANLGMTRTPDVHFFSEARHNGTKTVVFSPDFSMVSKYAD
                     QWIPVHAGQDGAFWMAVTHVILKEAHAEKQTGYFIEYTKKYTDAPFLVELVKEDDRWL
                     PGRLLRASLLEKYRNTENGDWKFLNLDENTGDFICPSGSSGHRWQNGTGQWNLKYRDG
                     ETGSEYNPVLSLIDRKDAILEVEFADFARDARSRRGVPVKYLKTREGETAFTTVYDLI
                     MGQYGVDRGLGGDSPSSYDDADSAYTPAWQEIYTGISSATVIRFAREWYRTAEATRGR
                     CMVIIGAGVNHWYHSNLIYRAATMALLLTGCPGVNGGGLNHYVGQEKLAPFDSWGAIM
                     SGKDWNIPARFQQSPIWHYIHSDQWRYDGNQADYNTVPENDFSSRHSADLIVKAVRNG
                     WMPFFPQYDRNNFEIASDAKEAGAMDDKGIQDYVISRLKKGELNYSVSDPDSEENFPR
                     VWFIWRGNALMASAKGHEYFLKHYLGTHHNEIADEVAKELVREIEWKENAPEGKMDLV
                     VDLNFRMDTTALYSDIVLPAASWYEKADLNTTDMHSFINPLAPAIPPVWEAKSDWAIF
                     REIARTTSEIAKKHFPGPVLDIVNVPIGHDTRGEISQPSIKDWYTGECDPVPGKSMHN
                     IVSVERDYTRIYDKFISLGSNVRKGLEAHNITIPCEDFYERLLSDKSHVNRIDGETFP
                     SIGDDTEVINAILLLSSVTNGTLSGRAFSSLEKNTGIDLSEVNPVNNDITVCFNDLQA
                     QPRRLLNSP"
     gene            complement(3010..3825)
                     /locus_tag="PLZ75_11905"
     CDS             complement(3010..3825)
                     /locus_tag="PLZ75_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOS73137.1"
                     /translation="MQTDLTKISLFALCLFLYSVSPVLRAQDGGEKIFESNCSACHTI
                     GGGRLVGPDLSGVYRIRNNEWLISFIRSSQQLIKSGDSAAVAIYEEYNRVPMPDNRLS
                     DAQILGVIDYIKASDSDVQAAGAVPEIPEDSLAVIYSTEYAAAGRALFYGHSAFANGA
                     APCLSCHNISDQSFLGGGRMARDLTASYGNLGAAGITAIVRNSPFPAMKTALSGHDIN
                     DDEIQALISLLKAAGEQTYRTRQPGLIFFSLGLIVALILIAHLYIFYDGRRIP"
BASE COUNT         1044 a         1093 c          941 g         1118 t
ORIGIN      
        1 ataggagaat tcagcagtct ccttggctgc gcctgcagat cattgaaaca gacagtgata
       61 tcattgttaa cgggattaac ctcactcagg tcaattccgg tatttttttc cagcgaagag
      121 aaggcccttc cgcttagagt accgtttgta accgatgaaa ggagaaggat cgcattaatg
      181 acttccgtat catccccgat ggagggaaat gtttcaccgt ctatccggtt tacatgactc
      241 ttgtcactga gcaggcgttc atagaaatcc tcgcagggaa tggttatatt atgggcttca
      301 agtcccttgc gaacgttgct tcccagactt atgaacttat catatattct ggtataatct
      361 ctttcaacac ttacgatatt gtgcattgat ttacccggga cggggtcgca ttctcccgta
      421 taccagtcct ttattgaagg ctgggaaatt tctccccttg tatcatgacc gatgggcaca
      481 ttaacaatat cgagcaccgg gccgggaaaa tgttttttag ctatctcact cgttgttctg
      541 gcaatttcgc ggaatattgc ccagtcgctt ttcgcttccc acacgggagg aattgccgga
      601 gccagcggat tgatgaaaga atgcatgtcg gtggtgttca gatcggcttt ttcataccat
      661 gatgctgccg gaagaacaat atcggaataa agggccgtgg tgtccattct gaaattcaga
      721 tccaccacca ggtccatctt tccttcagga gcattttctt tccattcaat ctcccttacc
      781 agctcttttg ccacctcgtc ggctatttca ttgtgatggg tcccgaggta gtgcttcagg
      841 aaatattcat gaccttttgc actggccatt agggcattgc ccctccatat gaaccacact
      901 ctcgggaaat tctcctctga atcaggatct gaaacagaat agtttaattc tcccttctta
      961 agccggctga tcacataatc ctgaatgccc ttgtcgtcca tggcacctgc ctcttttgca
     1021 tctgatgcta tttcaaagtt gttcctgtca tactgaggaa aaaatggcat ccagccgttt
     1081 ctcaccgcct ttactatcaa atcggctgaa tggcgggaag aaaaatcatt ttccgggacg
     1141 gtgttgtaat cagcctgatt accgtcatag cgccactgat cggaatgaat gtaatgccat
     1201 atgggcgact gctgaaacct ggccggaatg ttccagtcct ttcccgacat gattgcaccc
     1261 catgaatcaa acggtgcaag cttctcctgg cccacatagt gattcagacc gccgccgttg
     1321 actcccgggc agccggtaag cagcaaagcc attgttgctg cacggtagat aaggttgctg
     1381 tgataccagt gattcactcc tgcccctatt ataaccatgc acctgccccg ggtggcctct
     1441 gcagtccggt accattcccg ggcaaaccgg attacagtgg ctgaacttat tccggtatag
     1501 atctcctgcc atgccggggt ataagcagaa tcagcatcat cataggatga agggctgtca
     1561 ccgccaaggc cccggtcaac cccgtactgt cccataatga ggtcataaac agtagtgaac
     1621 gccgtctctc cttcccttgt cttcaggtat ttcacaggga caccccgcct ggatcttgca
     1681 tcccgggcaa aatcggcaaa ctcaacttca agtattgcat cttttctgtc aatcagcgaa
     1741 agtaccggat tatattctga tcctgtttca ccgtcacggt atttcaggtt ccattgtccc
     1801 gtgccgtttt gccatctgtg gcctgaactg ccggaagggc agataaaatc acccgtgttc
     1861 tcatcaagat tcagaaattt ccagtctccg ttctccgtat tacggtattt ctccagtagg
     1921 cttgccctta acaatctccc tggaagccac cggtcatcct cctttaccaa ttccacaaga
     1981 aaaggcgcat cggtatattt ttttgtgtat tcaataaaat agcctgtctg tttttcagca
     2041 tgagcctctt tcagtataac gtgggtcaca gccatccaga aggcaccatc ctggcctgca
     2101 tgaaccggta tccactgatc ggcatatttt gaaaccatgc tgaaatcggg tgagaaaacc
     2161 acggttttgg ttccgttatg acgggcttcg gaaaagaaat ggacatcggg agtacgcgtc
     2221 atgcccaggt ttgcgcccat cacggcaatg aacctggcat tgtaccagtc ggcactctcc
     2281 ccgacatccg tctgttcacc ccacacctcc ggaaaggaac tgggcagatc gcagtaccag
     2341 tcataaaaac ttagatgcac accgccaaac aactgaagaa atctcgatcc tgcggcaaag
     2401 ctaatcatcg acatggcggg aatgggagaa aacccgacta ccctgtcagc accgtatttt
     2461 ttggccgtgt aaatgtttgc agccccgatc agctccatta cctccgtcca tgcaactctc
     2521 ctgaaaccgc ccttgccgcg ggccttctgg tattttctcc ttttttcagg atcattctga
     2581 atgttttgcc aggccagaag aggatctccc gtctttttcc tttcttcatt gaacaaatca
     2641 agaagggcgc ctctgatcat gggatattta acccgaacgg gactgtagag gtaccaggag
     2701 aatgatatcc ctctctggca gcctctcggt tcatatggcg gaagagaatt ctcaagccgg
     2761 ggataatcaa gggcctggag ttcccatacc actattccct ccttgacatg aacattccag
     2821 ccgcagcctc cggtacagtt caccccgtga gtactgcgta ccaccttgtc atactgccag
     2881 cgattacgat aaaactcttc ccattcccgg gtctggggtg atatcatatc ttttatccaa
     2941 ctcatgattc ctgtttttta atcagcataa cttttcccgg atgccgccat ccggacttat
     3001 ttttaaaact cacggaattc ttctcccgtc ataaaaaata tagagatggg caatcagtat
     3061 cagtgccact atcagaccca gactgaaaaa gatcagtccc ggctgcctcg tgcggtatgt
     3121 ctgttccccg gctgctttca ggagagagat cagggcctgg atttcatcat cgttgatgtc
     3181 atgaccgctg agggcagttt tcatagcggg gaagggtgag tttctgacaa tggcagtgat
     3241 tccggccgcg cccaggttgc cataggaggc ggtcagatcg cgggccattc ttccccctcc
     3301 cagaaaggac tgatcactga tattgtgaca ggaaaggcat ggggcggcac cgttggcaaa
     3361 agcgctgtga ccataaaaga gtgccctgcc tgcagctgca tattcagtac tgtagataac
     3421 ggccagactg tcttccggga tttcaggaac ggcgccggca gcctgaacat cggaatctga
     3481 agctttaata tagtcaatta caccgagtat ctgtgcatca ctaagccggt tgtcaggcat
     3541 cgggaccctg ttgtattcct cataaatcgc aactgccgca ctgtcgcccg atttgatcag
     3601 ctgctgcgat gaacgtataa agcttatcag ccattcattg ttccttatcc ggtaaacacc
     3661 gcttaaatct gggcccacca gcctccctcc gccaatggta tggcacgcgg aacagttcga
     3721 ttcaaaaatt ttctcaccgc cgtcctgagc ccgcagaacg gggctcactg agtaaagaaa
     3781 aaggcatagg gcaaacaggg agatctttgt caaatccgtt tgcatgaaat ggttaattat
     3841 aggttattta ctgaaaagtc cggtcatcat gatcctcctt tatctgagca ggagcctggg
     3901 ttaatacgct aaaaaaacta cttttcgggt tatcagtatt ttgccttatt tttagcaaaa
     3961 ttataaggca tcctcacaaa gatatttgtt tttttaaacc ggcaaatgat aaaaccttaa
     4021 atatttcagg ctttccggaa atatcttatg cctgaaagaa aaagcgccag accgattccc
     4081 aggtaaacca ccgagagata ctgcctggga acgggggaat gacgcagccc gattcccatg
     4141 gaaaccatga tggctatgat gagatagctt ttgccggaga tgaaggaaaa gacaca
//
LOCUS       DAOSPX010000294         4165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19261_length_4165_cov_4.715085, whole genome shotgun sequence.
ACCESSION   DAOSPX010000294 DAOSPX010000000
VERSION     DAOSPX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19261_length_4165_cov_4.715085"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..650
                     /locus_tag="PLZ75_11910"
     CDS             90..650
                     /locus_tag="PLZ75_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73138.1"
                     /translation="MKRILLFAAVVSLIAGQLNAQDAVFAKGDKVLNLGLGLGTSLYH
                     GRYWKSQIPPVSASLEFGVADNIIDEKGLIGIGPYLGYTSYKYQVVDWGWKYSNFILG
                     VKGNFHYLFVDNLDTYAGILLGYNVSSSKEFGVITPGYDYSYSAGGLIYGGFVGARYY
                     FKESFAVMAEAGYGLTYLTLGVSLKL"
     gene            complement(778..1404)
                     /locus_tag="PLZ75_11915"
     CDS             complement(778..1404)
                     /locus_tag="PLZ75_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOS73139.1"
                     /translation="MKLKIENILEKEADAIRNIPLSNNYNGITDLIHHHVHKLKGKLI
                     ISGMGKVGQVADNIATTFCSTGTPAVFLHPSEAQHGDLGIIQENDILLVISNSGKTRE
                     IIELIDLAKLLYPSIPVVAITGNENPSFREKLEYIVLTGNPREVCPLDLTPTTSTTVM
                     TVIGDILVVLMMEKIRFTAGEYAKRHHSGYLGDKSRQGGQTQPGGMAD"
     gene            complement(1510..2340)
                     /gene="kdsA"
                     /locus_tag="PLZ75_11920"
     CDS             complement(1510..2340)
                     /gene="kdsA"
                     /locus_tag="PLZ75_11920"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOS73140.1"
                     /translation="MKTIIPKIPDLKHADNEKSFLLIAGPCVVESEDLLDTVAGHLAG
                     LSDKYKIPLILKASYRKANRSRGDSFQGIGDIKALGILKRISSKYSVPAVTDIHNPDE
                     AEIAAGYVDVLQIPAFLCRQTDLLEAAARTGKWLNIKKGQFLSGSAMKFAVTKVHNAG
                     NRNVMLTERGTTFGYQDLVVDFRNIAEMKENKVPVIVDITHSLQQPNQPSGVSGGNPG
                     MIEIIGRAAIAAGADGIFIETHPDPASARSDGENMLKLDKMEYILKQLIAIKNAINSL
                     "
     gene            complement(2577..3200)
                     /locus_tag="PLZ75_11925"
     CDS             complement(2577..3200)
                     /locus_tag="PLZ75_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOS73141.1"
                     /translation="MKKILILICLGYVSLIAVNAQVLGVKGGLSFATGRYVIYEFESS
                     SGNLFGVSGGILGEVPLSDALSLGTGISFVKKGARTDVYKIPVRYLEFPINLTYRLDF
                     ISWKLFAQAGPYAAAGISAKRKNNVSNKISFGSRASQFKRMDYGINLGAGFEIGNLQI
                     GINYGFGFLNISHAYREVIRNRLLTVSAVYYMEDVGYTLGNLWRSIF"
     gene            complement(3422..3595)
                     /locus_tag="PLZ75_11930"
     CDS             complement(3422..3595)
                     /locus_tag="PLZ75_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73142.1"
                     /translation="MKKNYFLIELIKSVPFRIFIILVLIITLIVVISNRREKTIRMKE
                     DVLYLPPRQEQQK"
     gene            3910..>4165
                     /locus_tag="PLZ75_11935"
     CDS             3910..>4165
                     /locus_tag="PLZ75_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOS73143.1"
                     /translation="MNTFILAESNWKTLQDAHIEVAILPWGATEAHNLHLPYSTDNIE
                     TERIAREAARKAFEKGAEIIVLPVIPYGVNTGQLDIRININ"
BASE COUNT         1088 a          881 c          907 g         1289 t
ORIGIN      
        1 tctataattg atatactttt ggagcaattg aaattcaaga attttcatac acctgatgaa
       61 aagtagtgtt taatttaatt tataaattca tgaagagaat tttattgttt gcggctgtcg
      121 tcagcttaat tgcgggacaa ctaaatgctc aggacgctgt tttcgctaaa ggagacaaag
      181 tccttaacct tggtctgggt ctgggtactt ccctttatca cgggcgctat tggaagagtc
      241 agattccacc tgtgtcggca tcactcgaat ttggggttgc agataacatt attgatgaga
      301 aaggtctgat tggtattggt ccctatcttg gttacacgtc ctataagtac caggttgtgg
      361 actgggggtg gaaatattcc aatttcattc tgggagtaaa gggaaatttt cactatctgt
      421 ttgttgataa tctggacact tatgcaggaa ttttactggg ctacaatgtg tcaagttcca
      481 aggaatttgg cgtgataacc ccgggttatg attattcata ttcggccggt ggtcttattt
      541 atggagggtt tgtcggcgca aggtattatt tcaaggaaag tttcgcggtt atggccgagg
      601 ccggttatgg tcttacctat ctcactctgg gcgtttcact gaaattgtag aaacagtttc
      661 ggggttatgg caggaacagc ctgaagtttt tcctggcata ccccgggcat tatttcattg
      721 aaactgtagt tgtattgccg cagtggggta aacaagcccc ggaatgataa ccggtggtta
      781 atctgccatc cctccgggct gcgtttgccc tccttgtctt gacttgtcac ccaggtatcc
      841 gctgtgatgc cgtttcgcat attcacctgc agtaaatctg atcttttcca tcattaaaac
      901 aaccaggata tcacctataa ccgtcatcac tgttgttgat gttgtcgggg taagatcgag
      961 ggggcatacc tcccggggat tgcctgtcag aacaatgtat tcaagctttt cgcggaatga
     1021 cgggttttca tttcctgtaa ttgcaacgac aggtattgac ggatacagca gttttgccag
     1081 gtcaatcagt tctattattt ctcttgtttt tcctgaattc gatatcacca gaagaatgtc
     1141 attttcctgg atgattccga gatccccgtg ctgagcttcg ctgggatgga gaaatactgc
     1201 cggcgtaccg gtgctgcaga atgttgttgc aatattgtcc gcaacctgtc ccactttacc
     1261 cattccgctg atgatcagct ttcctttaag tttatgaaca tgatgatgaa tcaggtcagt
     1321 tattccgtta taattatttg acagggggat gtttctgatt gcatcagcct ccttttcaag
     1381 aatattttct atttttaatt tcatgcgacc ggattttatg gaatgatccc ttcaaaggcc
     1441 ggtaaattac taaaaattgg cttcacagaa cttgcttcgg tgcttatagt cagaatcatc
     1501 attccggttt cataagctgt ttatagcatt tttaattgca atcagctgtt tgagaatgta
     1561 ttccattttg tcgagtttca gcatgttttc gccgtcagat ctggcagagg caggatcggg
     1621 atgtgtttca atgaatatac catctgctcc ggcggcaatt gcagccctgc cgattatttc
     1681 aatcattccc ggattgcctc cgcttactcc tgatggctgg tttggctgtt ggagggaatg
     1741 tgttatgtca acaattaccg gcaccttgtt ttccttcatt tcagctatgt tccgaaaatc
     1801 cacgacgagg tcctgatatc cgaaggttgt gcctctttct gtaagcatta cattcctgtt
     1861 gcccgcgtta tgtactttcg ttactgcaaa cttcatggcg cttcccgaaa ggaattgccc
     1921 cttcttgata tttaaccatt tgcccgtacg ggctgccgct tccagaagat ccgtctggcg
     1981 gcacagaaag gctggtatct gcaatacgtc aacatatccg gcagcaattt cagcttcatc
     2041 aggattatgt atatcggtta cagcggggac ggaatattta ctgctgatcc ttttcaatat
     2101 accaagggct tttatatcgc cgataccttg gaatgaatcg cctctcgatc tgttggcctt
     2161 ccggtaagaa gcttttaaaa taagggggat tttatatttg tccgacaacc cggccagatg
     2221 tccggccaca gtatcgagca gatcttctga ttccaccacg caggggccgg caatgagcag
     2281 aaaggatttt tcattgtccg catgtttcag atccggtatt ttgggaatta tggttttcat
     2341 agaatcattt ttctgttcct ggtcagtttt actacgtttc agattcccgt ccggttttaa
     2401 acaggaaaat cttcactggc tcagttttcg gttattgata tgaaaaaaaa ttgctctaaa
     2461 ttaacaaatt gctgaaacag aaaacatggt ttttcccgga aaactgatac gggctgcaga
     2521 tttggcccgg tacagtgagc acaggttaca agatttcagg aggtctgaag aaagggttaa
     2581 aagatactgc gccagaggtt gcccagggta tatcccacat cttccatata gtacacggca
     2641 gataccgtca ggagcctgtt cctgattact tccctgtaag cgtgggatat gtttaagaat
     2701 ccgaaaccat agtttatgcc gatctgcaga ttgccgatct cgaaaccagc acccagattt
     2761 attccgtaat ccattctttt aaactgtgat gccctggaac cgaaactaat tttgttgctt
     2821 acattgtttt ttctttttgc tgaaataccc gctgccgcat aaggcccggc ctgtgcaaac
     2881 agtttccatg aaatgaagtc cagcctgtat gtcagattta taggaaattc aagatatctt
     2941 acaggtattt tatacacatc agtcctggca ccttttttaa caaatgaaat accggtgccc
     3001 agtgacaaag catctgaaag cggcacttcg cccaatatcc cgccgctgac cccgaacagg
     3061 tttcccgacg atgattcgaa ttcatagatt acatatcttc ctgttgcaaa agataaacct
     3121 ccctttactc caagtacctg tgcatttacg gcaataaggc ttacgtatcc caagcaaatg
     3181 aggatcagta tcttcttcat attacgttta cactatggaa acaagcgata aataatttta
     3241 gtttaaattt atgaaataag tttgtcagtt acaaatcaat ctgcagggca cttggttttg
     3301 ccggtattat ggtcggttta atggctgaca tcccggccgc aaggaaagat tttgcagata
     3361 acgaaaccgg accttttccg cttatccggg ccgttaaata tcagccggga gggcggtctt
     3421 cttatttctg ctgttcctgt ctgggtggca gatacaagac atcttccttc attcttatgg
     3481 ttttttccct ccggtttgaa atcactacaa taagtgtaat aataagtaca agtatgataa
     3541 agatccggaa aggcacactt tttatcagct cgatcaggaa ataattcttt ttcataagaa
     3601 cttctttatc agttaggtat ttaaaacgta aaaatatcaa agaagagaga ataaagcaaa
     3661 tatttactca tgcatattgt ttttactctt tatatttgtt cccggatcac gtatttgccg
     3721 tgcatcagga aggaacaaca gccggcctgc gctcaggcgg tttatccgga tatccgggat
     3781 atacagtctt tattccgcta tccggaatat tatatgtgtt ttacggagga acccggcgta
     3841 tggttgagga agccacgaag gccggcaatg aaacgaaaaa cgatattcat attttaaaga
     3901 atggaaatca tgaatacttt tatccttgcg gaaagtaact ggaaaacact acaggatgcc
     3961 catattgagg ttgcaatact tccctgggga gctacggaag cccacaacct tcatcttccc
     4021 tacagcaccg acaatattga aacagagaga atagcaaggg aggcagcccg caaggccttt
     4081 gaaaaaggtg ctgaaataat tgtccttcct gttatacctt atggagtcaa tacgggccag
     4141 ttggatatcc ggatcaatat aaata
//
LOCUS       DAOSPX010000295         4141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19397_length_4141_cov_3.723201, whole genome shotgun sequence.
ACCESSION   DAOSPX010000295 DAOSPX010000000
VERSION     DAOSPX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19397_length_4141_cov_3.723201"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PLZ75_11940"
     CDS             <1..811
                     /locus_tag="PLZ75_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS73144.1"
                     /translation="GMVGLQLYSIRAAMAKDPLGSLRKLADMGYVYVEHANYINRKFY
                     GYTPSEFRKVLDDLGLKMISGHTVMGRQHWDESKQDFSDSWKYTVDDAAVLGQQYVVS
                     PSMDNSMRNNYDDFRKYMDVFNKCGELCRKQGMKFGYHNHDFEFSEKLNNEKLFDIMM
                     RSIDPDLVVVQLDIGNLYNGGAVALDVVKQYPGRFENIHVKDEIESSGGREKYESCVL
                     GEGIVNARKVTKLATKIGGTKVYIIEQESYQGRDPMDCMRENLAVMKKWGY"
     gene            complement(789..1646)
                     /locus_tag="PLZ75_11945"
     CDS             complement(789..1646)
                     /locus_tag="PLZ75_11945"
                     /inference="COORDINATES: protein motif:HMM:NF039685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HOS73145.1"
                     /translation="MNALTTEEINTILQNIESAEKSSVLGYFSRPFRVYHGDRALFIK
                     LYFPVNNQEYISRIIRNHNDYVRELRFAGIKIPETIILSRKSNRKHQLVIIQDSFRDD
                     ELLRNRILSEDLEGVKQLCTMVFDEMLQFREKRNKDIDIGFHPTLRNYCVHENKLWFF
                     DTFPPMLMNQKALNRLIIKMSPHGGFLRKIIPPRLINRVTDEYYNLDTMFTGVVGSCC
                     RLRPHDSEAILEFSREYANNSPLITDDEKKSILRLLVRPPQLSGIWILVRKLSRNTGR
                     PNVSIPTSS"
     gene            complement(1679..2518)
                     /locus_tag="PLZ75_11950"
     CDS             complement(1679..2518)
                     /locus_tag="PLZ75_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOS73146.1"
                     /translation="MNIRIDYDLCTRCDLCSRVCGSRRIIIADDKRPVKLIEAGCNDC
                     GHCIAICPVNAIVNTRTDMSAFTAMTDPDIRYEQFSNLVRNRRSIRNYRKQPLKQEHI
                     DKLMEVARYIPSGSNKQLLKYRFITDETLLQEIKTEMADKMRMVLKLSASFPVRYFVK
                     DRSKSSASRLVKLFFEDGVDTFLRGAPCLLVIYTEQKYFRIPQWDAGIAANTIDLAAQ
                     TLGIGTLMNGYFVELANRFKSVRKLVRLNGREQVLTALCLGYPSVRYKRTVYRRPPEI
                     SNL"
     gene            complement(2693..3133)
                     /locus_tag="PLZ75_11955"
     CDS             complement(2693..3133)
                     /locus_tag="PLZ75_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73147.1"
                     /translation="MKKVFLLSVIMALILSACGKSPKNYPEGTWKMVQMQMIEKGKKT
                     NYFSDRYKVSQIKMWSGKHFMFVGKYEVDTTSSYRYGVGTFTLDGNIYEETIMYHFDQ
                     AYEGNKNKIWLEIRNDTLIHIFPVDNSGKPDQNRHWCERYIRLQ"
     gene            complement(3422..>4141)
                     /locus_tag="PLZ75_11960"
     CDS             complement(3422..>4141)
                     /locus_tag="PLZ75_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73148.1"
                     /translation="LQKKDLSEILSEAEEFTINELLPALARGAFIEDQQKQHIAEKIS
                     YYSGISIKSVLQLNLNIPTSFFWKELLRDKNGKTLGRLDSRYLGTDKTEAGSSPDYNA
                     EMSAWLDAFTPAINHYMREHLNFRTDLKYYMFGPTHPWSWDTGNSNIRENLRQAMARN
                     PRLRVMFQTGYYDGATTYFSSKYMMWQLDPGGRLKDRLSFRGYRSGHMMYLREEDIKT
                     ANEDLRTFIREGAPDGRPAKY"
BASE COUNT         1036 a          919 c          968 g         1218 t
ORIGIN      
        1 gggaatggtt ggactgcagc tgtattccat ccgtgccgcc atggcaaagg atcccctggg
       61 atcgctcaga aagctcgcag atatgggcta tgtatatgtt gagcatgcca attacattaa
      121 ccgcaaattc tacggataca ccccttccga attcaggaag gtgcttgatg atcttggcct
      181 gaaaatgatc agcggccata ctgtaatggg ccgccagcac tgggatgagt cgaaacagga
      241 tttttccgac agctggaaat atacggtgga cgatgccgcc gtgctggggc agcagtatgt
      301 ggtgagtccc tccatggaca acagcatgcg gaacaattat gacgatttca ggaaatacat
      361 ggatgtattc aacaagtgcg gtgaactgtg caggaagcag ggaatgaaat tcggctatca
      421 caatcacgat tttgagttca gcgaaaaact caacaatgaa aagctttttg acataatgat
      481 gaggagcatt gatcctgatc ttgtggttgt ccagctcgat atcggcaatc tttacaacgg
      541 gggcgcagtt gcactcgatg ttgtgaagca atatccggga aggtttgaga atatacacgt
      601 aaaggatgaa atagaatcaa gcggcggcag ggagaaatat gaaagctgtg ttctgggaga
      661 agggatagtc aatgcccgaa aggtaaccaa actggctaca aagatcggcg gaacgaaagt
      721 ctatatcatt gaacaggaat cataccaggg aagggatccc atggactgca tgagggaaaa
      781 ccttgccgtt atgaagaagt ggggatactg acattgggtc tgccggtgtt tcgcgataat
      841 ttcctgacaa ggatccagat acctgaaagc tgaggcggtc tgacaagcag cctgaggata
      901 ctcttttttt catcatccgt gatgaggggt gaattgtttg catattccct gctgaattcc
      961 agaatggcct cagagtcatg gggccttagc ctgcagcagc ttccaaccac ccctgtgaac
     1021 attgtatcca ggttgtagta ttcgtcagtc accctgttga tcagccgcgg aggaatgatt
     1081 ttcctgagga agccgccgtg gggagacatt ttgattatca gtctgttcag ggctttctgg
     1141 ttcatcagca tgggagggaa ggtatcaaaa aaccatagtt tgttttcatg aacacagtag
     1201 tttctcagtg taggatgaaa cccgatatcg atgtccttat tccttttctc cctgaactga
     1261 agcatttcgt caaaaaccat ggtgcaaagc tgtttcactc cttccaggtc ttcactgaga
     1321 atccggtttc gcaacaattc gtcatccctg aatgaatcct gtattattac aagctgatgt
     1381 ttcctgtttg acttccttga cagtattatt gtttcaggga tttttattcc ggcaaatctg
     1441 agctccctga catagtcatt atggtttctg ataatacggg agatatattc ctggttgttc
     1501 acggggaaat agagtttgat aaaaagggcc ctgtcgccgt gataaaccct gaatggtctt
     1561 gagaaataac ccaggaccga ggatttttct gccgattcaa tattctggag aattgtattg
     1621 atctcctctg ttgttagcgc attcatggca atattccgca cacaatatgt tgtttatatc
     1681 acagattact gatttcagga ggtctgcggt aaactgtcct tttgtaccgt actgacgggt
     1741 agcccaggca taaggcagta agcacctgtt ccctgccatt gagccgtacc agttttctga
     1801 ccgacttaaa cctgtttgca agttctacaa aatatccgtt catcagagtc cctattccaa
     1861 gagtctgggc tgccagatca atggtgttgg cggcaattcc cgcatcccac tggggtatgc
     1921 ggaaatattt ctgttcagta tagattacca gaaggcacgg agcgcctctg agaaaggtat
     1981 caaccccgtc ttcaaaaaag agttttacca gtcttgatgc actgctttta cttctgtcct
     2041 taacgaaata ccttacagga aagcttgcgg aaagcttcag aaccattctc atcttatcag
     2101 ccatttcagt ctttatttcc tgaagtaggg tttcatccgt aatgaatctg tatttcagca
     2161 gctgtttgtt tgatcccgac ggaatgtatc gggcaacctc catcagctta tcaatatgct
     2221 cctgtttaag aggctgtttc ctgtaattcc tgatacttct cctgtttctt accaggtttg
     2281 agaactgttc atagcggatg tccggatcag tcatggcggt aaatgcactc atgtcggtcc
     2341 tggtgttgac aatggcattt acaggacaaa tggcaatgca atgcccgcag tcgttgcatc
     2401 cggcttcgat aagctttacc ggccttttgt catcagcgat gattatcctc cgggaaccac
     2461 agactctgct gcataagtcg catcttgtgc ataaatcata gtctatcctt atgttcatag
     2521 agtgttggga gctttttcaa aagctcaaaa ttagtcaaag tccggtttta tacaacaaac
     2581 tccggattat tcctgtgaac agctgtgatg gcagcaggca gcagagatcc tttcccctcc
     2641 ggcgctgtta cgcccccagg caggggtatg ggttagaaaa tgtttcctga ttttattgca
     2701 acctgatgta tctttcgcac cagtgcctgt tctggtcggg ttttccgctg ttgtcaacgg
     2761 gaaatatgtg tatgagggtg tcatttctta tttcaagcca tattttattc ttatttccct
     2821 cataggcctg atcgaaatgg tacataatag tttcttcata aatattaccg tcaagtgtaa
     2881 aggtccccac gccatatctg tagctggatg ttgtgtcgac ctcatattta cccacgaaca
     2941 tgaaatgctt tcctgaccac attttgatct ggctcacctt gtagcggtca gaaaaataat
     3001 tggttttctt tcctttttca atcatctgca tctgaaccat tttccaggtg ccttccggat
     3061 agtttttggg actcttgccg catgctgaca atatcagcgc catgatgaca ctaagaagaa
     3121 aaactttttt cataatttca ggttttagtt atttactgtg atagccggct cctattcagt
     3181 aaatttacta aacaagacaa tacgagtcaa taaaaaccgg tatttaatga gggtgatcca
     3241 gtgcgaattc atgctgaaaa gtgctgaaac aacagtttgt tcaggctgat tccaaatacc
     3301 accggtgtac ggctggcgat gtactgctgg cgatgtactg ctgacgttgt actgctgaca
     3361 atatatgcct gacgatgtac gactgatacc gccggatatg gcatgttaca gacggtatga
     3421 tttaatattt cgcaggccgg ccgtccgggg caccttccct gatgaaggtc ctcagatcct
     3481 cgttggccgt ttttatatcc tcttcgcgca ggtacatcat atgcccgctg cggtaccccc
     3541 tgaatgacaa gcggtctttc agccttccgc ccggatccag ctgccacatc atgtattttg
     3601 aactgaaata ggtggttgct ccgtcatagt atcctgtctg gaacattacc ctcaaacgcg
     3661 gatttctggc cattgcctgt ctcagattct cccgaatgtt actgtttccc gtatcccacg
     3721 accagggatg cgtagggccg aacatgtaat atttcaggtc ggtcctgaaa ttcagatgtt
     3781 cacgcatgta gtggtttatt gcaggagtga aggcatccag ccaggccgac atttcggcat
     3841 tgtaatccgg agagcttccc gcttccgttt tatctgttcc gagatatctc gaatcgaggc
     3901 ggccgagtgt ttttccgttt ttgtccctga gcagttcttt ccagaaaaag cttgtcggaa
     3961 tattcaggtt gagctgtaat accgatttta tggagattcc tgaataatat gagatttttt
     4021 cagcaatgtg ttgtttttgc tgatcttcaa tgaaggctcc tctggccagg gccggcagca
     4081 gttcgtttat tgtgaactct tcggcttcgg acagaatttc tgaaagatct tttttctgaa
     4141 g
//
LOCUS       DAOSPX010000296         4136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19422_length_4136_cov_3.518500, whole genome shotgun sequence.
ACCESSION   DAOSPX010000296 DAOSPX010000000
VERSION     DAOSPX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19422_length_4136_cov_3.518500"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PLZ75_11965"
     CDS             <1..1038
                     /locus_tag="PLZ75_11965"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOS73149.1"
                     /translation="ADILLENGISYFEIGHPVNDRTLSISNGSFSEIFDINELRDIWF
                     RTSYLLDRRQTPPDLAHERFINYSGHVLKYNMNGFRGTYRSLGISPSRRKRSGIRAAI
                     IREKGTNGDREMAYALFLAGFDVRDVHMTDLTEGRETLEDINMLVFAGGSTNSDVFGA
                     AKGWAAAFRYNRVAAQILEDYYKREDTLSLGVCNGCQLMVELGLIYPEHGNRPRLLHN
                     KSGKFESAFLAVRITPNNSVMLGDMAGMELGIWLAHSEGRFDLPLDEEKYVIPVKYYY
                     ESYPGNPGGSDYNAASLCSADGRHLGLMLHPERSIFPWQCAYYPPDKQDDKVTPWIKM
                     FVNARKWIESQ"
     gene            complement(1097..1651)
                     /locus_tag="PLZ75_11970"
     CDS             complement(1097..1651)
                     /locus_tag="PLZ75_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73150.1"
                     /translation="MRRGFFYLPAIVFVFAIIASCTGSGKRPDSTNGLSINKIWQQPD
                     GTISLHLDKADCYSDVANPSENTAEWDVVVSRSGRYNVWLSSATLDTTDLNYKNKVMI
                     SVQDTRIEGLPECDRIVRNAEDVAYPYYRADSFIGSMYIKDTGEFQIQVISEQILPKE
                     KFNGDVSGEDLSKLLSVSFTPVKR"
     gene            complement(1796..3703)
                     /locus_tag="PLZ75_11975"
     CDS             complement(1796..3703)
                     /locus_tag="PLZ75_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOS73151.1"
                     /translation="MKFDPFVIPFNIGLFFILIYAVVRSIIWFRALSRPDKLRLQRGF
                     FGRAFGQSLKEIFLESLLHRKIFRTNFWLGYMHMSLAFGWFLLILFGTIEADIFGDSH
                     LNPPYKAIFFKFFNPVHGMKGIEAAYSFLMDLILAFILSGLLLAIIKRFYSRVVGMRK
                     TTKLKVLDKIALASLWMIFPSRLLAESFTSGAYGSGSFLTGSLGSSLASFLPAIEMAY
                     PFWWLYSLSLGTFFILLPLTRYMHIPTELFLIFARNSGIRTGDQPDAFMEMQIYSCSS
                     CGICIDACQLNFSTGITNIQAAYLLKGLRNDEDVRDIAYNCLMCGRCDVKCPVGIELT
                     PVRMIARRSQETETKEKNGIWKKYFPDRHHPASSKQSNEPSYSWLPEAEAPKADIVYY
                     AGCMTHLTPAIKNAMVKILDASGLKYDFIDRDGGVCCGRPLMLAGQDKEARELINYNS
                     QAILKSGATTLVTSCPICYKVFRESYYLNVEVLHHTQFIRRLILKGTIRLNFLRKTVT
                     YHTPCELGRRSGIYDDPRLVLMHVARFQKTGFDDENSLCCGGSLGNTRISQEQKNKIA
                     SDAAYELTKTDPDILATACPLCKKTFAGVSDTPVADIAEIVAEALSLPVPLADNEERK
                     TLKQRETANIL"
     gene            complement(3693..4037)
                     /locus_tag="PLZ75_11980"
     CDS             complement(3693..4037)
                     /locus_tag="PLZ75_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS73152.1"
                     /translation="MGDFGFTVLKERHINYDANDKSIAEYIAEREPSFRICIECGCWS
                     ATCTTGNFIRFSLRELQILLKRGENEIVREQIDRCMLCGKCILVCPRGVNTRNVILLA
                     REAFRKSVENEI"
BASE COUNT         1143 a          963 c          907 g         1123 t
ORIGIN      
        1 gcagacatac tgctagagaa tggtatttca tactttgaaa ttggtcatcc ggtaaatgac
       61 agaaccttgt caatatccaa cggcagcttt tctgaaatat tcgatatcaa tgaactgcgt
      121 gacatatggt tcaggacttc ctatcttctt gacaggcggc agactcctcc tgacctggca
      181 catgaaagat tcattaacta ttccggacat gtgctgaaat ataatatgaa cggattcagg
      241 ggaacttaca ggtcactcgg catttcaccc tcacggagga aaagaagcgg tatcagggca
      301 gctatcataa gagagaaagg cacaaacggt gacagggaaa tggcttatgc acttttcctt
      361 gcaggttttg acgtgaggga tgtccacatg acagatctca cagagggaag ggaaacactg
      421 gaggatataa acatgcttgt ttttgcaggc ggatctacaa actcggatgt attcggagca
      481 gcaaagggct gggcagctgc tttccgttat aaccgtgttg ctgcccaaat ccttgaagac
      541 tactataaaa gagaagatac actgtctctc ggtgtgtgca acggctgcca gctgatggtg
      601 gaacttggac tgatatatcc ggagcacggt aacaggcccc gcctgctgca taataaatcc
      661 ggaaaatttg aatctgcctt tcttgcagtc cgtatcaccc cgaataattc cgttatgctt
      721 ggcgatatgg caggaatgga actcgggata tggctggccc acagtgaagg gcgttttgac
      781 ttacctcttg acgaggagaa atatgtaatt ccggttaagt attattatga atcctacccc
      841 ggtaatcccg gaggatcgga ttataacgct gcatcccttt gttcggctga cggcaggcat
      901 cttggcttga tgcttcatcc cgaacgttcc atatttccct ggcagtgcgc ttattacccc
      961 ccggataaac aagacgacaa ggtcactccc tggataaaga tgtttgtaaa tgccagaaag
     1021 tggattgaat cgcaatagcc ccgggaaagc taaaaaaagg gctgcaaacc gcagccctca
     1081 tcagtaagaa tgccttttat cttttgacag gtgtgaatga gaccgaaagg agctttgaca
     1141 agtcctcgcc ggaaacatct ccattaaatt tttccttagg caggatctgt tcgcttatca
     1201 cctgtatctg aaattctcct gtgtccttta tatacattga accgataaat gaatccgccc
     1261 tgtaatacgg atatgcaaca tcttccgcat tcctgactat cctgtcacat tccggcagtc
     1321 cttcaatacg cgtatcctga acacttatca taaccttgtt tttgtagtta aggtcggtgg
     1381 tgtccagggt tgcacttgac agccagacat tgtaccgtcc ggatctggaa accaccacat
     1441 cccattcggc agtattctct gatggatttg ccacgtcact gtaacaatcc gccttgtcaa
     1501 gatgcaatga tatggttcca tccggctgct gccatatttt atttatgctg agaccattgg
     1561 tggaatctgg tcttttgcct gaaccggtac atgaagcgat aatggcaaaa acaaatacaa
     1621 tcgccggtaa ataaaaaaat cctcttctca tgttaaatgt tgaaaaaatc agtttgcaag
     1681 taaaacgtaa caaaaatcat tccattgtgc aggaaataat tttaattgaa aacattttcc
     1741 ggatcagaaa tgttcaattg aggtgatgga aaatgaagat caggctatta ccttttcata
     1801 agatatttgc agtttccctc tgtttcaagg tttttctttc ctcattgtca gccaggggaa
     1861 cgggcaggga aagagcttca gcaacaatct cggcaatatc cgcgacaggt gtatcagata
     1921 ctcctgcaaa agttttcttg cagagcgggc aggcagttgc aagaatatcc ggatcagtct
     1981 ttgtcaattc atatgccgca tcactggcta ttttattttt ctgttcctga cttatcctcg
     2041 tatttccaag gctgccacca cagcacaggg agttttcatc atcaaaaccg gttttctgga
     2101 aacgtgcaac atgcatgaga acaagcctgg gatcatcata aatacccgat cttctcccca
     2161 gttcgcatgg tgtatgatag gttaccgttt ttctcaggaa attaagcctg atcgttcctt
     2221 tcagtatcag tctccggata aactgggtgt gatgtaatac ctccacgtta aggtagtagc
     2281 tttccctgaa aaccttgtag catatcgggc aggaagtgac aagggttgtt gctcccgact
     2341 tcaggatagc ctgtgaatta tagttaataa gctccctggc ttccttatcc tggccggcaa
     2401 gcatcagagg gcgtccgcag caaacccctc cgtccctgtc gatgaaatca tatttcagac
     2461 cggaagcatc cagaatctta accattgcat ttttaatggc cggcgttaga tgtgtcatgc
     2521 agccggcata ataaacaata tcggctttcg gcgcttctgc ctccggcagc cagctgtacg
     2581 acggctcatt actttgtttt gaggaggcag gatgatggcg gtcagggaag tattttttcc
     2641 agatcccgtt tttttccttc gtttcagttt cctgtgaccg ccgggcaatc attcttacgg
     2701 gtgtcagttc aattcccacc ggacatttta catcacaacg gccgcacatc aggcagttgt
     2761 aggctatatc acgaacgtcc tcatcatttc ttagcccctt gagcagatag gccgcctgga
     2821 tattggtaat tcctgtggag aagttaagct ggcaggcatc aatgcatatt ccgcatgagg
     2881 aacatgaata gatctgcatt tccataaatg catccggctg atcgccggtt ctgatcccgg
     2941 agttcctggc aaaaatcagg aataattccg taggtatatg catatacctt gtaagcggaa
     3001 gcaggatgaa aaaagtaccg agagacagcg aatagagcca ccagaacgga tatgccattt
     3061 caattgccgg aagaaatgaa gccagtgatg atccgaggga accggtaaga aagcttccgc
     3121 taccataggc cccgcttgta aagctttctg ccagaaggcg gctgggaaaa atcatccata
     3181 atgaggccag ggctatcttg tcaagaacct tcagttttgt tgtttttctc attccgacaa
     3241 cccgggaata aaatcttttt attatggcaa gcagcagtcc tgaaagaata aaagccagaa
     3301 taagatccat caggaaggaa taggcagctt caatcccctt cattccgtga accggattga
     3361 agaatttgaa aaatatggcc ttatagggag gattcagatg tgaatccccg aaaatatcag
     3421 cttctattgt tccgaataaa atgagcagga accacccgaa tgccaggctc atatgcatat
     3481 aacccagcca gaagtttgtc ctgaagattt tcctgtgtaa aagactttca aggaatatct
     3541 ccttcaggct ttgtccgaat gcccttccga aaaatccccg ctgcagcctc agcttgtccg
     3601 ggcgtgaaag agccctgaac cagatgatgc tccggacaac ggcatagatc agtatgaaaa
     3661 agagaccaat gttaaacggt atgacaaaag gatcaaattt cattttccac cgattttctg
     3721 aatgcttccc tggcaagcag gatcacattc ctggtattca ctcccctcgg acatacaagt
     3781 atgcatttac cgcaaagcat acacctgtca atctgctccc tgacaatctc attttcaccg
     3841 cgtttaagca atatctggag ttccctgaga ctgaacctga taaaatttcc cgtggtacag
     3901 gttgcagacc agcacccgca ttcaatgcat attctgaaac tgggttccct ttcagcaata
     3961 tattccgcaa tgctcttgtc attggcgtca taattgatat gtcgttcctt cagtactgta
     4021 aatccaaaat ctcccatatc agacttaaat atttctgaaa taatccatta catcagctac
     4081 agccgcccgg gcatgagata ttgtttcggt aatattcatc ggtgcagtac aggctc
//
LOCUS       DAOSPX010000297         4133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19442_length_4133_cov_4.615007, whole genome shotgun sequence.
ACCESSION   DAOSPX010000297 DAOSPX010000000
VERSION     DAOSPX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19442_length_4133_cov_4.615007"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLZ75_11985"
     CDS             <1..268
                     /locus_tag="PLZ75_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73153.1"
                     /translation="PPYDGRNTLSRLIARTFREKILRDDSGSDAPVRTYEIASAGING
                     ISRLFGLEMSLTGITDSQGDLKSLYFSSRLVKFNAPLKKDESLQ"
     gene            339..1310
                     /locus_tag="PLZ75_11990"
     CDS             339..1310
                     /locus_tag="PLZ75_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOS73154.1"
                     /translation="MKKLIMILPAIVFLVQGFGQNTSSARDSGLKKTAASDSSDAARL
                     ILGKDLIRIEDDREALRIRVGNRGVTILESLEAGERRICFDDYRRDGKNDEEALFTGG
                     GRDNMRDEGRTRRLRFRGHWAGVEAGFNNYLTPGNSLNLPENIDYMSLHSGKSRFFNA
                     NVAQTSLGLTRRIGFVTGLGINWNNYRFDGNNNIIKLENGEIEKYDPELLLKRSKFST
                     LYLTLPLLLEVQIPANYSQLSIAAGPIGAVKLYSYSKMVFDNGDKVRSEDDFSLNMLR
                     YGATLRIGFGNFQVFGTSYFMPLFQTGKSPGGVDLYPFEVGISFTID"
     gene            1365..2078
                     /gene="pepE"
                     /locus_tag="PLZ75_11995"
     CDS             1365..2078
                     /gene="pepE"
                     /locus_tag="PLZ75_11995"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOS73155.1"
                     /translation="MNLLLISNSTNPGEAYLSYPRHNIREFLGPRALDVLFIPYAAVT
                     FSYDLYEEKVAERFREIGHRITSVHHFPDPVKAVMEAEAIAVGGGNTWKLLKNIRENK
                     LTVPVREKVRGGTPYIGWSAGANVACPTIMTTNDMPVTRPPSFRSFNLVPFQINPHYL
                     DFSPEGHAGETREQRITEFIEMNPDVYVVGLREGTMLEISDGSIRLIGPRKARIFKKG
                     ISAVELGDSDDFSWLLKSR"
     gene            2151..>4133
                     /locus_tag="PLZ75_12000"
     CDS             2151..>4133
                     /locus_tag="PLZ75_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73156.1"
                     /translation="MKLVSKILRPLAFVISRIMAVMFVAAMLVQDKVADIILMSLSNN
                     ISTKFETGSVRLSFIRRFPKASLDLKNVVVRSSPGFDRKCFGRTDTDTLLAAGSVKME
                     FRIRNIINGYYNIERIRVRNARLKILTDTSGMVNYEIRSLNQATQGNELVINLDGINL
                     SNVETIYDNRATKLIISGIMENGRIRSRITGNNIDFTGKGDLKIDLFSLYDFRISKNI
                     SSRVNVILNKSDEGIRFEKSSFVFDDNEFIMDGFVSADNLLDISVKGENIDIAGLKAY
                     LPDRFLEKAKEYNPRGMLALDGTIRGKVSRTVNPGINIAFSLKNGNVSYKPSSSGIQN
                     VSFDGSFTNGREMTPGTSTLKIGNFRGRLGSSDYTGSLVLSDFTSPDCSLELKGRLIA
                     GEIAEFFNLNTVSAAEGSVDMHIRTKGKIPAIRNLSLTDIFGIINEGRLGFNSFGMNL
                     NNNRIRIGNVTGQILFSGNITAKDLDLTYMGQRMRINGSFTGLPQWAAGKNATLTASG
                     SLSSDRISLDAFFPEKTGQGSEEENKKPFAFPDNVILDFGFSIADLEYRDFRARNVSG
                     KMSYKPRTVNFNNLNINSLDGLISGDGYFLQNADKSYIMKGGFSIEKVNVKKAFIVFN
                     NFGQNFIRSENLEGLLSGSLAILIPMDPELKASARTV"
BASE COUNT         1226 a          945 c          981 g          981 t
ORIGIN      
        1 accgccatat gacggcagga ataccctgag cagactcatt gcaagaacct tccgtgaaaa
       61 aatcctgcgg gatgacagcg gaagcgatgc tcccgtcaga acatatgaaa tcgcatcagc
      121 cggaattaac ggcataagcc gtctgttcgg gctggaaatg tcactcaccg gcattactga
      181 ctcacaggga gatttgaaat cactttattt cagttcaaga cttgtgaaat tcaacgcccc
      241 tctgaaaaaa gacgaatcat tgcagtaact tttaaaacag cattacgtcc catctgtaca
      301 gccaaactcc tacaatcatg aaaactgctt ttaccaaaat gaaaaaatta ataatgatac
      361 ttcctgccat agtcttcctg gtacagggat tcgggcagaa cacttcatcg gccagggaca
      421 gcggtctgaa aaagactgca gccagtgaca gcagtgatgc cgcaagactg atactgggca
      481 aggacctgat aagaattgag gatgaccgcg aggccctcag aatcagggtg gggaacaggg
      541 gtgtgaccat actcgaatcc ctggaggcag gtgaacgcag gatctgcttt gatgattacc
      601 gcagggacgg aaagaatgat gaggaagccc ttttcaccgg cggcggacgg gataacatgc
      661 gcgatgaggg acgtacccgc agattgcgtt tcagaggaca ctgggcagga gtggaagccg
      721 gattcaacaa ttacctgaca cccggcaaca gtttaaacct tccggaaaat atcgactaca
      781 tgtcgctaca ttcgggaaaa tcccgctttt tcaatgcaaa cgtggcgcag acaagcctgg
      841 ggctgaccag gagaatcggt tttgtaaccg gtctcggtat aaactggaac aactacaggt
      901 ttgacggaaa taacaatatt ataaaactgg aaaacgggga aatagaaaag tacgatcctg
      961 agcttttact gaaaagatcc aaattctcaa cactttacct gaccctgccg cttttactgg
     1021 aagtccagat ccctgccaac tacagtcagc tcagcatagc agccggaccg ataggcgcgg
     1081 tgaaactgta ttcatattcc aaaatggttt ttgacaatgg cgacaaggtc aggtcagaag
     1141 atgatttcag cctgaacatg ctgagatacg gggcaaccct gcgtataggg ttcggaaatt
     1201 tccaggtatt cgggacttcc tatttcatgc cgttgtttca aaccgggaaa agccccggag
     1261 gggttgatct ctatcccttc gaggtcggga tttcttttac tatagattaa gcctgacaag
     1321 ccgtacagat atgctatctt tgcacagtca aaatatctgt ccggatgaat cttttactga
     1381 taagcaattc aaccaacccc ggagaggcat atctctccta tccgaggcac aacatccgcg
     1441 aattccttgg cccccgggcc cttgatgtac tttttatccc ctatgctgcg gtaacatttt
     1501 catatgacct ctatgaagaa aaagtagcgg aaaggtttcg tgagatcggt caccggatca
     1561 cctccgttca tcatttcccg gatccggtaa aggcagtcat ggaagcggaa gccatagccg
     1621 tcggcggcgg aaacacctgg aaactcctga aaaatatcag ggaaaacaag cttaccgtac
     1681 ctgtcaggga gaaagtcagg ggaggtacac cctatatcgg ctggagtgcc ggtgccaacg
     1741 tggcatgtcc caccataatg acaacgaacg acatgccggt aacaaggcct ccttccttca
     1801 gatcattcaa ccttgttccc tttcagataa atccgcatta tcttgacttc agtccggaag
     1861 ggcatgccgg agaaacacgg gaacaaagaa taaccgaatt cattgaaatg aatccggatg
     1921 tttatgttgt aggattacgc gaagggacca tgctggaaat cagtgacgga agcattcgcc
     1981 ttatcggtcc gcgtaaagcc aggatattca aaaagggaat cagcgctgtt gaactcgggg
     2041 acagtgatga tttctcatgg ctgctcaaaa gcagataata caaagattcg cgcaaataaa
     2101 ttaactttgt ccaccgttat ccggatacaa atcatttttc cggcaggcga atgaagttag
     2161 tgtcaaaaat actcaggcct cttgcttttg tcatttcaag gatcatggca gtaatgtttg
     2221 tggctgcaat gctggtacag gacaaggttg cggatatcat actcatgtcg ctgagcaaca
     2281 atatttcaac caaatttgaa acgggctcgg tcagactgtc cttcatcaga agattcccga
     2341 aggcctccct tgacctgaaa aatgtagtgg tccgttcttc accgggattt gacaggaaat
     2401 gcttcggcag aacagatacc gacaccctgc tggcagcagg ttccgtaaaa atggaattca
     2461 ggatcaggaa tatcatcaac ggctactaca atatcgaaag gatccgcgtc aggaacgcac
     2521 ggctgaaaat actgaccgac acttccggaa tggttaatta tgagataagg tccctaaacc
     2581 aggccacgca gggcaatgaa cttgtcatca atctcgacgg tatcaacctc agcaatgtgg
     2641 aaaccatcta tgacaaccgc gcaacaaagc tgattatctc aggtatcatg gaaaacggca
     2701 ggataagaag caggatcacg ggtaacaata tcgacttcac cggcaaaggc gatctgaaga
     2761 ttgacctgtt ttccctttat gatttcagga tcagtaaaaa catcagttcc agggtaaatg
     2821 tgattctgaa caagtctgat gaaggaatca gatttgaaaa gagcagtttt gttttcgatg
     2881 ataatgaatt catcatggac ggatttgttt ctgctgacaa tcttctggat atctcggtga
     2941 aaggggaaaa tattgatatt gcgggtctga aagcatatct gccggacaga ttcctggaaa
     3001 aggcaaagga atataatccg cgcggaatgc tggcactgga cggcacaatc aggggaaagg
     3061 tctcaagaac cgtcaatccg ggcataaaca tcgccttttc ccttaaaaac ggcaatgtca
     3121 gctacaagcc atcctcttcc gggattcaaa atgtttcatt tgacgggtcc tttacaaacg
     3181 gccgggaaat gacgcccggg acaagcacgc tgaagatcgg caacttcagg ggcagactcg
     3241 gatcatcaga ttataccgga tcacttgtcc tttccgactt cacttcaccc gactgcagcc
     3301 ttgaactgaa gggcaggctc atagcagggg aaatcgcgga attctttaat ctgaatacag
     3361 tttccgcagc tgaaggatct gttgacatgc atatcagaac aaagggaaaa atacccgcga
     3421 tcaggaattt aagccttacg gacatctttg gcataatcaa tgaaggaaga cttggattca
     3481 actcctttgg catgaacctg aacaataaca ggatacgtat tggaaacgtg acggggcaga
     3541 tcctcttttc cggcaatata actgcaaagg acctggatct cacatatatg ggccagagga
     3601 tgagaataaa cggcagcttt accggcctgc cacagtgggc agcaggtaaa aatgcaacat
     3661 taacagcgtc gggatcactg agctctgaca ggatttccct tgatgcattc tttcccgaga
     3721 aaaccggcca gggcagtgag gaggaaaaca aaaaaccctt cgcatttcct gacaatgtaa
     3781 ttcttgattt cggcttcagc atcgctgatc tggaatacag ggatttcagg gcccggaatg
     3841 tgtcgggaaa aatgagctac aagccccgta cggttaattt taataatctt aatattaatt
     3901 cactggacgg tcttatctca ggtgatggtt actttcttca gaatgcagat aaatcctata
     3961 taatgaaggg tggtttcagc attgaaaaag tgaacgtaaa aaaggctttt attgttttca
     4021 ataatttcgg ccagaatttc atccggtctg aaaacctgga aggcctcctg tcaggttccc
     4081 ttgcaattct tattcccatg gaccctgaac ttaaggcctc ggcaagaaca gta
//
LOCUS       DAOSPX010000298         4120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19521_length_4120_cov_3.910947, whole genome shotgun sequence.
ACCESSION   DAOSPX010000298 DAOSPX010000000
VERSION     DAOSPX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19521_length_4120_cov_3.910947"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="PLZ75_12005"
     CDS             <1..1200
                     /locus_tag="PLZ75_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HOS73157.1"
                     /translation="GGEGNCIAYYSYNDIMKLEKFMQGKPIAEQEIGKQLLLETVSYA
                     ESSVCRRKLLLHYFGEEYTPDNCEACDNCLHPKAQFEGSESIVNVLETVLAVKEKFKA
                     DHIANILSGKVTSAIKSYKHNKLEYFGTGEDKDEKYWNMVIRQALIAKFLTKDIENYG
                     LLKVTREGLAFVDNPVSFMLAEDHDYADSDEEDNAFGAKTAAVDEELFSILKDLRRKI
                     SKQKGVPPFVIFQDPSLEDMAIQYPVNLDELQNISGVGAGKAQRYGREFVDLIKKYVE
                     EKEIIRPLDMVVKSVVNKSGIKVYIIQSIDMKRSLEDIADAKDLEMGELLSEIEAIVN
                     SGTRLNLDYYINEVIEDDRQEEIYSYFREEAESDSLEEAINELGNDFEEEEIRLMRVK
                     FLSDMGN"
     gene            complement(1241..2368)
                     /locus_tag="PLZ75_12010"
     CDS             complement(1241..2368)
                     /locus_tag="PLZ75_12010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS73158.1"
                     /translation="MYWLLLLISIPYLYIILRVYKSLRGIRSFTAASTPDTFISVVAA
                     CRNEEASLPFLLADIAAQDYPPSLFEFIISDDNSSDKTHDVASGFPGIINLRVLRNRG
                     SGKKAALRTAVSEARGNLVVAVDADSRMEPGWLKTIVSFHEKEGSDMIICPVMLEAEG
                     SGFFLRFQELEFLGLQAITAGSAAAGDPLMCNGANLFFTKKSFERHAFHLHDELASGD
                     DIFLLHRMKADGSSRISWLESADARVRTNALHSPAEYLRQRARWISKAGSYKDLSTKF
                     TALATLAVVLLQPAALIAAFAGIRFLLLYPALLLAKSLPDYLVLRNTAVRYGKRYLLK
                     WFIPSQLIYPFYVIAVAACALTGRKAGTHRKQAGQKAGREF"
     gene            2555..3034
                     /locus_tag="PLZ75_12015"
     CDS             2555..3034
                     /locus_tag="PLZ75_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73159.1"
                     /translation="MQKTDRLLAIEKILATEKISSHEELLWKLRAEGIVCTQATLSRN
                     LRQLGAVRRPDPGGGYRYVLPESSHVRPAAAGRLQLLPVITDLTNTGGMLLIKTLPGN
                     ASNVAIFIDNADRHEIAGTIAGDDTILVIARGGVSVEGIHDCLETLLPGLHEWLRDR"
     gene            3120..>4120
                     /locus_tag="PLZ75_12020"
     CDS             3120..>4120
                     /locus_tag="PLZ75_12020"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOS73160.1"
                     /translation="MKEKVVLAYSGGLDSSIILKWLVEKGYEVIAFIADVGQDTDFEE
                     SRGKAEMLGASEVIISDLKKEFVENYVLKAIMANAIYENRYLLGTALSRPLIASEQIS
                     VARRVGAGTVAHGATGKGNDQVRFELCYYALMPGIRVISPWKDRDFLASFKGRTDLLK
                     YASENKIPVKASIDKPYSEDENLMHISHRAGILEDPMYSIHKDMLVKMVMPQDAPDKE
                     TRIVIHFRNGLPVRLVNKEDGTMMEDPYDMFVYLNELAGRNGIGLLDMVENRFVGIKS
                     RGVYESPAATVFHIAHRDIEGIAMDREVMRLVSMLTPKFAELIYNGFWFSPEMDFLMA
                     "
BASE COUNT         1121 a          901 c         1124 g          974 t
ORIGIN      
        1 ggcggtgagg gaaactgcat agcctattac agctacaatg acattatgaa gctggagaaa
       61 ttcatgcagg ggaaacctat tgccgaacag gaaataggga aacagctcct gcttgagaca
      121 gtttcatatg ccgaatcatc ggtttgccgg agaaaactgc ttctccacta tttcggtgag
      181 gaatataccc cggataattg cgaggcatgt gacaactgcc tgcaccccaa ggcccagttc
      241 gaaggcagtg aatcaattgt gaatgttctg gaaacggttc tggcggttaa ggaaaaattc
      301 aaggccgacc atattgcaaa tattttgtcg gggaaagtta catccgccat caagtcgtat
      361 aagcacaaca agctggaata cttcggaacg ggggaagaca aggatgagaa atactggaac
      421 atggttataa ggcaggccct tatcgccaaa ttccttacaa aggacataga aaactacgga
      481 cttctgaaag ttaccaggga agggctggca tttgtggata atcccgtgtc atttatgctc
      541 gcggaagatc acgattatgc cgattcagac gaggaagaca atgcattcgg tgcaaaaaca
      601 gctgccgttg atgaggagct gttcagtatc ctgaaggacc tgaggagaaa aatatcaaag
      661 cagaagggag tgcctccctt tgtgattttc caggatccct cactcgagga tatggccata
      721 cagtatccgg tgaacctgga tgaactccag aacatttccg gagtgggtgc aggcaaggca
      781 cagagatacg gcagggaatt cgtggatctg ataaagaaat atgttgaaga aaaggaaatt
      841 atcaggcccc ttgacatggt ggtcaagtcg gtggtgaata agtcgggaat aaaggtttac
      901 attatccaga gtatcgacat gaagcgttcc ctcgaggata ttgccgatgc caaggatctt
      961 gagatgggtg agctgctttc cgaaattgaa gcaattgtca attcgggaac caggctgaat
     1021 ctcgattact atataaatga agtcattgaa gatgaccgcc aggaggaaat ttattcctat
     1081 ttcagggagg aagcagaatc agattcgctg gaagaagcga taaacgagct cgggaatgat
     1141 tttgaggagg aagagataag actgatgcgg gtcaagtttc tttccgatat gggtaactga
     1201 ccggtgctga atcatcagcc cggtacgctg cctgctgccg tcagaactcc ctgcctgcct
     1261 tctgccctgc ctgcttcctg tgcgttccgg ccttccttcc agtcagggca caggctgcca
     1321 ctgcaatcac atagaaagga taaattagct gggagggaat aaaccatttc agcaggtatc
     1381 ttttcccgta gcggacagct gtatttctca gtacaagata gtcaggcagc gatttagcca
     1441 gaagcagggc cggatacaga agcagaaacc tgattccggc aaatgcggca atgagtgccg
     1501 cgggctgcaa aagaaccaca gccagagtgg ccagtgccgt aaatttcgtg gacagatctt
     1561 tgtaggaacc ggctttcgag atccatcttg ccctttgtct cagatattcc gccggtgaat
     1621 ggagagcatt cgtccttacc ctggcatctg ccgattccag ccaggaaatc cgggaagatc
     1681 cgtcagcttt catgcggtga agaagaaaaa tatcgtcgcc cgatgccagt tcgtcgtgaa
     1741 gatggaatgc gtgcctctca aatgatttct tagtaaaaaa caggtttgcc ccgttgcaca
     1801 taaggggatc ccctgctgca gccgatcctg cggttatggc ctgaaggccg agaaattcca
     1861 gttcctggaa gcgcaggaaa aaaccactgc cttcggcttc aagcataacc ggacagataa
     1921 tcatgtcaga cccttccttt tcatggaatg acactatcgt cttcagccac cccggctcca
     1981 tgcgggagtc ggcgtcaacc gcaacgacca ggtttccccg ggcttcagac acagcagtcc
     2041 tgagggccgc tttcttaccg ctgcccctgt tcctcaggac tctgagattt attattccgg
     2101 gaaaccctga cgcaacgtca tgggttttat cagaggagtt atcatcgctg ataataaatt
     2161 cgaacaggga aggaggatag tcctgagcgg caatgtcggc cagcaggaag ggcagactgg
     2221 cttcctcgtt cctgcaggct gcgacaaccg aaatgaaggt gtccggtgtg gaggcagcag
     2281 tgaaggatct gattcccctg aggcttttgt acaccctgag gataatatat aaatatggta
     2341 tgcttatcag aagaagaagc cagtacattg tttatttcat catccgttcc cgaaaccgtg
     2401 ctgtatgatg cccttaccct gatgcgtgcc ttttcgccgg atgttgatga tccgtcaatc
     2461 agcgggcggg aagctgttca aaaaataaac aatcttccgg tgggcatttg tttctgcaaa
     2521 aatatgaata tatttgcggt ctttgtgaat aaatatgcaa aaaacggaca gattgcttgc
     2581 cattgagaag atactggcaa cagagaagat atcgtcccac gaggaattgc tgtggaagct
     2641 ccgtgctgaa ggcatagtct gtacacaggc aactctttca aggaacctgc gtcagctggg
     2701 agctgtcaga aggcctgatc cggggggcgg ttacagatat gttctcccgg aaagcagtca
     2761 tgtccgcccg gcagctgccg gacgtcttca gttgctgccc gtgataacgg acctgacaaa
     2821 cacgggggga atgcttctga taaagacttt gccgggcaat gccagcaatg tggccatttt
     2881 tattgacaat gcggacaggc atgaaatagc cggtactatt gcgggtgatg acaccatact
     2941 tgtaattgcc aggggcggcg tttccgttga agggattcat gactgcctcg agacgctcct
     3001 gccgggattg catgaatggc tcagggacag atgatggttt cggcgggaag cccggaaact
     3061 gcaggaccgc ggaatcatca ccggatcagg tataaggatg taaattaaaa atattcagga
     3121 tgaaagaaaa ggttgtattg gcttacagtg gcggcctgga ttcatcaatc atactgaaat
     3181 ggctggttga aaagggttat gaggtgattg cctttattgc cgatgtgggc caggacaccg
     3241 attttgagga atcccgcggg aaggcagaga tgctgggagc ctcagaggtg attatttccg
     3301 accttaaaaa ggaatttgtt gaaaactatg ttctgaaggc aataatggca aatgccattt
     3361 atgagaacag gtatttgctg ggaactgccc tgtcaagacc tctgatcgcg agtgaacaga
     3421 tatcagttgc aaggagggtg ggtgccggta ccgtggctca tggtgcaaca gggaagggaa
     3481 atgaccaggt gaggtttgag ctgtgttatt atgccctcat gcccggaata cgggtgatat
     3541 ctccctggaa ggacagggat tttcttgcca gcttcaaggg gagaacggat cttctcaaat
     3601 atgcttccga gaataagata cctgtcaagg ccagtatcga caagccttac agcgaagatg
     3661 agaacctgat gcatataagt caccgggcag gtattctgga agatccgatg tacagcattc
     3721 acaaggacat gctggtgaag atggttatgc ctcaggatgc cccggataag gagacaagga
     3781 ttgtcattca tttcaggaac ggtcttccgg tgagactcgt gaacaaggaa gacggaacca
     3841 tgatggaaga cccctacgac atgttcgttt atctgaatga actggccggc aggaacggaa
     3901 tcggtcttct tgacatggtg gaaaacaggt ttgtcggaat caaatccagg ggtgtttatg
     3961 aaagtcctgc tgcaaccgtg tttcacattg cccacaggga tattgaagga attgccatgg
     4021 accgtgaggt tatgcgcctg gtaagcatgc tcaccccgaa attcgctgaa ttgatataca
     4081 acggattctg gttcagtccg gagatggatt tcctgatggc
//
LOCUS       DAOSPX010000299         4119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19529_length_4119_cov_4.376230, whole genome shotgun sequence.
ACCESSION   DAOSPX010000299 DAOSPX010000000
VERSION     DAOSPX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19529_length_4119_cov_4.376230"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /gene="groL"
                     /locus_tag="PLZ75_12025"
     CDS             complement(<1..1145)
                     /gene="groL"
                     /locus_tag="PLZ75_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOS73161.1"
                     /translation="MGKEIKYNIDARALLKEGVDQLTDAVKVTLGPKGRNVVLEKKFG
                     APQVTKDGVTVAKEIELSDPYKNMGAQMVKEVASKTGDIAGDGTTTATVLAQAIISVG
                     LKNITAGANPMDVKRGIDKAVEKVVESIKSQSQVVGDDLKKIEQVARISANDDEEIGH
                     LIAEAMSKVQKEGVITVEESKGTSTSVEVVEGMQFDRGYISAYFVTNTEKMEAELENP
                     YILIYDKKISSMKDMLPILEASAQTGKPLLVVAEDIEGEALATLVVNRLRGSLRVCAV
                     KAPGFGDRRKEMLEDIAILTGGTVISEEKGLKLEHATLDMLGTAEKVIVDKENTTIVN
                     GYGDKEMIQARVGQIKQQMSTTTSDYDKEKLQERLAKLAGGVAVLYIG"
     gene            complement(1173..1451)
                     /locus_tag="PLZ75_12030"
     CDS             complement(1173..1451)
                     /locus_tag="PLZ75_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOS73162.1"
                     /translation="MAQLKGKILAGKVLVKPNEAEAKTASGIIIPDSAKEKPRQGKVV
                     LTGASKKDEEMEVKKGDEVLYGKYSGQELTIDGEEYLLISQSDILFIM"
     gene            complement(1596..1958)
                     /gene="secG"
                     /locus_tag="PLZ75_12035"
     CDS             complement(1596..1958)
                     /gene="secG"
                     /locus_tag="PLZ75_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOS73163.1"
                     /translation="MYIFVTILVIIACILQVLVVLVQNSKGGGLAANFTSTGQTMGVR
                     KTADFLEKATWTLAASILLLSVVATAAIPRGGEAEQSRISTQINQAIDPNAIPTLPTA
                     VPPASSAGTTTGQDQPAN"
     gene            complement(1969..2940)
                     /locus_tag="PLZ75_12040"
     CDS             complement(1969..2940)
                     /locus_tag="PLZ75_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73164.1"
                     /translation="MNKNDFIRLMEGPGPDNRDLLGELGSLINVFPYFHSAHMLLLKG
                     LQNTSDVKFENQLRSSALHIANREVLYYYLTRKDAPGEDQYRKEEDQYRKEEDVPVAA
                     GTDMPPSGINGNNDTTDRQQVVIELAKNSEDFISELEKDDAAYGSDSGPKGYSIIITT
                     ESGDKESDASLIVVNEESGEIEERIVYMDPGFSFHEEDDLLELDTGYDKIADSVEPEV
                     PAGGKEYEGPPTVQQMQSELIDRFIIANPRIEPDRERDDIPPPDLGQTAAEEAEGLVS
                     ETLAKIYVKQGYYSRAIDIYEKLSLKYPGKSTYFASLILEIQELIKK"
     gene            complement(2946..3488)
                     /locus_tag="PLZ75_12045"
     CDS             complement(2946..3488)
                     /locus_tag="PLZ75_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOS73165.1"
                     /translation="MELICKIRKVLSYRMCPCLTAAVFILLLSGCKISYSFTGASISP
                     DVKTISIQYFQNRASLVQPGLSQNLTDALIDKCKAQTNLSFINGIGDVNFEGEISDYN
                     TRPLTVSADAQAATNRFTITVRVRFTNAVDPDLSYEQNFSRYEDYDSALSLADVEDSL
                     SRKIVELLVEDIFNRAFVNW"
     gene            complement(3472..>4119)
                     /locus_tag="PLZ75_12050"
     CDS             complement(3472..>4119)
                     /locus_tag="PLZ75_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS73166.1"
                     /translation="VDFVEKYRMPAIKADAEARIVLLNYSWPGNVRQLKNITEQISII
                     EKGREISASILKTYLPDYDGVKLPAVIKKEEDKTFASEREILYKILFDMKSDMNDLKK
                     LVYDLIQHGDTSITLGQPHTAVIENLFRDEVPSRTGPAVPEAPRVEFQPHQVRDDIQD
                     TEEIVEESLSLADKEVEMIRKALEKFNGKRKLAARELGISERTLYRKIKEYGIDL"
BASE COUNT          978 a         1055 c          817 g         1269 t
ORIGIN      
        1 ccgatataca gtactgcaac acctccggca agcttggcca gacgttcctg cagtttttcc
       61 ttgtcataat ccgatgtggt ggtgctcatc tgctgcttga tctgtccgac gcgggcctgt
      121 atcatttcct tatcgccgta tccgtttact atcgttgtgt tttccttgtc aacaatcacc
      181 ttttcagctg ttcccagcat gtcgagagtc gcatgctcca gtttcagtcc cttttcttcg
      241 gaaataaccg taccgccggt gagaatagct atgtcttcaa gcatttcctt ccttctgtcg
      301 ccgaatccgg gagccttcac ggcacatacc ctcagggaac cacgcaggcg gttgaccacc
      361 agggtagcca gggcttcacc ctcaatgtct tcagcaacaa ccaggagggg tttgccggtc
      421 tgggcagaag cttcaagtat cggaagcata tccttcatgg atgatatttt cttgtcatag
      481 atgagaatat agggattctc cagttctgct tccatctttt cggtgtttgt aacaaaatat
      541 gcagagatat agccgcggtc aaactgcata ccttccacaa cttccactga agttgatgtt
      601 cccttggatt cttcaacggt tataacgcct tctttctgaa ccttgctcat ggcttcggca
      661 ataagatgac ctatttcttc atcgtcattt gccgaaatcc ttgcaacctg ttcaatcttt
      721 ttcagatcat cacccacaac ctgcgattgt gatttgatac tttcaacaac tttctcaact
      781 gccttgtcga ttcctctttt tacatccatc ggattggcac cggcagtgat atttttcaga
      841 ccaacactta taatagcctg ggccagaaca gttgcagtcg tcgttccgtc accggcaata
      901 tcacctgtct tggatgcaac ttccttaacc atttgtgcac ccatattctt ataggggtcg
      961 gataattcta tttccttggc aacggtcaca ccgtccttgg taacctgcgg agctccgaat
     1021 ttcttttcca ggacaacatt cctgcccttc ggacccaatg taacttttac cgcatcggta
     1081 agctgatcaa caccttcctt cagaagtgca cgcgcatcaa tattgtactt aatctctttt
     1141 cccattgtat ttctgattta ttggttttct attcacatta taaaaagtat atctgactga
     1201 gaaataagga gatattcttc cccgtcaatt gttaattcct gtccggaata ttttccgtaa
     1261 aggacctcat cacctttctt aacttccatc tcttcatcct tcttggatgc tcctgtcagt
     1321 actaccttac cctgacgggg tttttccttt gctgaatccg ggattataat tccgctggca
     1381 gtcttggcct cagcctcatt cggtttaacc aaaacctttc ctgccagaat tttacctttt
     1441 agttgtgcca tgttatctag ttttaaagtt gaatatatga ttttactgtc tcgtgttagc
     1501 atatattatg ccaaagcagg aaacctgcca ttttgaaaaa aaaagtgtca gtctatgaca
     1561 aactgacact ttataaaatc atgagttctt cgcattcaat tcgccggctg atcctgaccc
     1621 gttgttgttc cggctgagga agccggggga acagcagtcg gaagggtcgg aatggcattg
     1681 gggtcaatgg cctgattgat ctgggtgctg attcttgact gttctgcctc tcccccgcgg
     1741 ggtattgcgg ccgtagccac aacactgagc agcagaatgg aagcagcaag ggtccatgtt
     1801 gccttttcaa gaaagtcagc agtctttctc actcccatgg tctgaccggt tgatgtgaaa
     1861 tttgctgcca gtccgccacc ttttgaattc tgcaccagta caacaagtac ctgcagaata
     1921 catgcaatga ttaccagaat cgtaacaaaa atataaattc ccattctctt atttttttat
     1981 aagctcctgt atctccagaa tcagagatgc aaagtaagtg ctttttcccg gatatttcaa
     2041 acttaatttt tcatatatgt ctattgccct tgaataataa ccctgtttta cataaatctt
     2101 cgccagtgtc tcactcacca gtccttccgc ttcctccgcg gcagtctgac cgagatccgg
     2161 cggaggaata tcatcccgct ccctgtcagg ttctattctc ggatttgcaa taatgaaccg
     2221 gtctatgagt tccgattgca tctgctgtac cgtaggaggt ccttcatatt ctttcccgcc
     2281 tgccggaact tccggttcta cagaatcagc aattttgtca tatccggtat caagttcaag
     2341 aaggtcgtct tcctcatgaa atgaaaatcc gggatccatg tatacaatcc tttcttcaat
     2401 ctcacccgat tcctcattaa ccacaatgag cgaagcatca ctttccttgt ctcccgattc
     2461 cgtggtaatt attatggaat agcccttcgg accgctgtcc gaaccatatg ccgcatcatc
     2521 cttttccagt tcactgataa aatcctcact gttcttcgcc agttctataa ccacctgctg
     2581 cctgtcggtc gtgtcattgt tgccgtttat tccggaaggt ggcatatctg ttccggccgc
     2641 aacagggaca tcctcttcct tcctgtactg atcctcttct ttcctgtatt gatcctctcc
     2701 cggggcatct tttcttgtca ggtaataata gaggacttcc cggtttgcaa tatgcagggc
     2761 ggagcttctg agctgatttt caaactttac gtcggaggta ttctgcagtc ctttcaggag
     2821 aagcatgtgg gcactgtgga aatacgggaa tacgtttatg agagacccca gctctcccag
     2881 gagatcccgg ttatcaggcc ccggaccttc cataagccgt atgaagtcat tcttattcat
     2941 cggtcttacc agttcacaaa agctctgttg aaaatatctt caaccagcag ttccacaatt
     3001 ttcctggaaa gagaatcctc aacatcggcc agtgaaagag cgctgtcata atcctcatag
     3061 cgcgaaaagt tttgttcata gctcagatcc ggatcaacag cattggtaaa tctcactctc
     3121 accgtaatgg taaacctgtt ggttgcagcc tgtgcatcgg cagatacagt cagaggcctt
     3181 gtattgtaat ctgatatttc cccctcaaag ttaacatcac ctattccgtt tatgaagctg
     3241 agattcgtct gagccttgca tttgtcgata agggcatccg taagattctg actaagtccg
     3301 ggctgaacca gactggccct gttctgaaaa tactgaatgc tgatagtttt cacatcaggt
     3361 gagatgctcg cccccgtaaa ggaatatgat atcttgcaac cgcttaaaag cagaataaat
     3421 acggcagcag tcaggcatgg acacatcctg tagctgagga ctttccttat cttacagatc
     3481 aattccatat tccttgattt tcctgtaaag ggtacgttcc gaaattccaa gttcacgggc
     3541 tgccagtttt ctcttgccgt taaatttctc cagggctttc cttatcattt caacctcctt
     3601 gtcagccagt gaaagtgatt cttccacaat ttcctcggta tcctgaatat catccctcac
     3661 ctgatgcggc tggaattcaa cacggggagc ctccgggaca gccgggcccg ttctggacgg
     3721 cacctcatcc ctgaatagat tttctataac ggctgtatgc ggctggccga gggtaattga
     3781 agtatcgcca tgctgaatca ggtcatatac cagctttttc aggtcattca tatcactctt
     3841 catatcgaag agtatcttgt aaagtatctc tctctcagag gcaaatgttt tatcctcttc
     3901 ctttttaatt actgccggaa gttttacgcc gtcataatcc ggaagatagg tcttcaggat
     3961 tgaggcagaa atttcccttc ccttttcgat gatggatatc tgctcggtaa tattcttcag
     4021 ctgccttaca ttaccgggcc aggaatagtt aagaagcaca atacgtgctt cagcatcggc
     4081 cttaatggca ggcattctat atttttcaac aaagtcaac
//
LOCUS       DAOSPX010000300         4110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19589_length_4110_cov_3.948459, whole genome shotgun sequence.
ACCESSION   DAOSPX010000300 DAOSPX010000000
VERSION     DAOSPX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19589_length_4110_cov_3.948459"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLZ75_12055"
     CDS             <1..381
                     /locus_tag="PLZ75_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73167.1"
                     /translation="IIDHWQTERPEKEGLWNIMTALTGLGDFDLGEAIWYLQEYPLDL
                     TDWSVMNSHRKDIEFIDPGFRGQTIKEVLPPDELPVSRHNANRFRLDRDGGGRSEYSA
                     GDIWLLPYWMGRYLGVIGQASGIN"
     gene            complement(480..3278)
                     /locus_tag="PLZ75_12060"
     CDS             complement(480..3278)
                     /locus_tag="PLZ75_12060"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOS73168.1"
                     /translation="MKKAIPVIFMAFALLSCSRDAGRNLTVQELRCNNLSDPGGSGPD
                     PVFSWILSSPLRGVSQNAYQILLAESAGDLKSEERCIWNSGRVVSGESVRVAYAGPEL
                     KPATKYFWKVRAWDNRDNPSGWSRPALFITGLFDGDDWEGAEWIGFEEVPDSMLLVPG
                     IHRGLQVEGHLAEARTVVPLFRREVSLDKKIAEAFVFVSGLGQYELHINGRRIGDSFL
                     SPGWTDYDKTCFYNTYEITDELKKGKNVIGAIVGNGFFNINRERYYKLVINYGAPRLK
                     LLLKIRYSDGSGETLKSDGLWKTAPSPVTFSSIFGGEDYDARLEQPGWNNADFDEASW
                     KNVLIVREPKGALKPEPDHPLKVMQTFSPRRIFSPASGIYIYDFGQNASGIINLRIKG
                     RSGQNVLLKPAELLGSDSLIMQKASGEPYWFSYTTGSGREVEWRPKFSYYGFRYVQVE
                     GAVPEGKPNPDNLPVITGLQMLHTRNSSPAAGSFECSNDLFNRIFSLIDWAIRSNLAS
                     VATDCPHREKLGWLEQTHLMGNSVKYVYDIHNLFSKMIDDMIEAQLDNGLVPDIAPEY
                     VEFVGGFRDSPEWSSACIILPWNLYEWYGDIDAVKRAYPMMKRYLDYLGSRSRNGILN
                     HGLGDWYDLGPEFPGVAQLTPIAVTATSIYYYDARLLAGMARILGEESDAAFYGELAE
                     RIRTAFNREFFNPLTKVYSTGSQTAYSMPLFFGMADDSLRSEVVNNLVKSINDGNRAL
                     TAGDVGYRYLLRVLEQEGHSGLIFDMNSKTDVPGYGYQMSKGATALTESWAGLAEVSN
                     NHMMLGHLMEWFFSGLGGIRQDENSKAFKHIVVSPEIAGDITRTDCSWKSVYGEISSS
                     WELSGNELTLKVRIPVSCTATIRFPQSDAAKIKLDGKAAGNSDDVYKIRNEGGKICLD
                     ILSGNYTFSCEMDAGKNF"
     gene            complement(3527..3925)
                     /gene="mscL"
                     /locus_tag="PLZ75_12065"
     CDS             complement(3527..3925)
                     /gene="mscL"
                     /locus_tag="PLZ75_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOS73169.1"
                     /translation="MKLIDEFKAFAMRGNVVDMAVGIIIGAAFGKIVSSVVNDIIMPP
                     IGLLLGGVNFTDLKLVMKAATDLEPAVTWNYGNFIQVTIDFLIIAFAVFMLIKGMNTL
                     KKKELEAPAPPPEPTKEEKLLTEIRDLLKK"
BASE COUNT         1019 a         1038 c          922 g         1131 t
ORIGIN      
        1 ataattgatc actggcagac cgagaggccg gaaaaggaag gactctggaa cataatgacc
       61 gcgcttaccg ggctcgggga ttttgatctc ggggaagcaa tatggtacct gcaggaatat
      121 cctctggatc tgactgactg gtcggttatg aacagccaca ggaaggatat tgaattcatt
      181 gatccgggat tcaggggtca gacaataaaa gaggttctgc cgccggatga gcttccggtt
      241 tcaaggcata atgcaaaccg tttccgcctt gaccgcgacg gagggggaag gtcggaatac
      301 agtgcaggcg acatctggct cctgccctac tggatgggac ggtatctggg tgtgataggc
      361 caagcttccg gaataaactg accaccagaa ttggaatcgt tattacccgg gaactgacag
      421 gcagcaataa tcctttaaca attccctgag actgaattcc tgtaagatgt ttaatccgtt
      481 cagaaatttt tcccagcatc catttcacag ctgaaggtat agttgcctga aaggatatca
      541 agacagattt ttccgccttc attcctgatt ttatatacgt catcagaatt tccggcagct
      601 ttcccgtcga gttttatttt tgcggcatcg ctttggggaa atctgatggt ggcagtgcat
      661 gaaaccggta tccttacttt cagagtcagt tcattcccgc tgagttccca tgaactcgaa
      721 atttccccgt aaaccgattt ccaggaacag tcagtccggg taatatctcc cgcgatctcc
      781 ggtgaaacaa caatgtgttt aaaggctttc gagttttcgt cctgtcttat tcctccgagt
      841 ccgctgaaga accattccat cagatggccg agcatcatat ggttgtttga aacttcggcc
      901 agtcctgccc aggattccgt aagggcagtg gcacccttag acatctggta tccgtagccg
      961 ggcacgtcgg tttttgaatt catgtcaaat atcagtccgg aatgtccttc ctgctcaagg
     1021 accctcagca gataacggta accgacatca ccggccgtaa gtgccctgtt accgtcgttg
     1081 attgatttta ccaggttatt caccacttca ctccggaggc tgtcgtcagc catgccgaaa
     1141 aaaagcggca tggaataggc agtctggctg cccgttgaat acactttcgt cagcgggttg
     1201 aagaattccc tgttaaatgc tgttctgatc cgttctgcca gttccccgta aaaggctgca
     1261 tcactttctt caccaagtat ccgggccatt ccggccagca gcctggcgtc atagtaatat
     1321 attgatgttg cagttactgc tatgggagta agctgtgcaa cccccggaaa ctccggtccc
     1381 agatcatacc agtcgcccag gccgtgattc aggattccgt tccgggaacg gctgccaaga
     1441 taatccaggt atcttttcat catgggatat gcccttttca ccgcatcaat atccccgtac
     1501 cactcataca ggttccaggg aagtatgata caggcactgc tccattcggg agaatcacgg
     1561 aaccctccca cgaattccac gtactcgggg gcgatatccg gaacaaggcc attatccagc
     1621 tgtgcttcaa tcatatcatc aatcatcttg ctgaacaggt tatgaatgtc atatacgtat
     1681 ttgacggaat tccccatgag gtgtgtctgt tcaagccatc ccagtttttc gcggtgcgga
     1741 caatcagtgg ccacacttgc aaggttgctt cgaatggccc agtcaataag actgaatatc
     1801 ctgttgaaca aatcatttga acattcaaag ctcccggccg ccggggatga gtttcttgta
     1861 tgaagcatct gcaatcctgt aataactgga agattgtcag gattgggctt gccttccggt
     1921 actgctcctt caacctgtac atacctgaac ccgtaatagc tgaattttgg tctccattcc
     1981 acttcccttc cggaacccgt tgtataggaa aaccagtatg gctcacccga cgccttctgc
     2041 attataaggg aatcggaacc gagcagttcg gcaggtttta ataatacatt ctgcccgctc
     2101 ctgcctttga ttctgagatt tattatgccc gatgcattct gtccgaagtc atatatataa
     2161 atcccggaag ccggactgaa aattcttcgc ggactgaaag tctgcatcac cttcagggga
     2221 tgatcaggct ccggtttcag ggctcctttc ggttctctta caatcagcac gttcttccat
     2281 gatgcctcat caaaatccgc gttgttccat ccgggctgtt caagtctggc atcataatcc
     2341 tctcctccga aaatactgct gaaagtaaca ggtgaagggg ccgttttcca tagtccgtca
     2401 gattttagcg tttcaccgga gccgtcactg tagcggatct tcagcaggag cttcagcctg
     2461 ggcgccccgt aattaattac aagcttataa tagcgttccc tgttgatgtt gaaaaagcca
     2521 ttccctacaa tggccccgat aacgttttta ccttttttta gttcgtctgt tatttcataa
     2581 gtattataga agcatgtttt gtcataatcc gtccagccgg gtgaaaggaa actatccccg
     2641 atcctgcggc cgttaatatg caattcatat tgacccagtc cgcttacaaa aacaaaagct
     2701 tcagctatct ttttgtccag gctgacttcc ctccggaaca ggggtaccac tgtccttgcc
     2761 tccgccagat gaccctccac ctgcagcccc cggtgtattc ccgggacgag cagcattgaa
     2821 tcgggaactt cctcaaaacc aatccattct gcaccttccc agtcatcccc gtcaaagagc
     2881 ccggttatga agagggccgg cctgctccat ccggaaggat tgtccctgtt atcccaggca
     2941 cggaccttcc aaaagtattt tgttgccggt tttaattcag ggccggcata tgcgaccctt
     3001 actgattccc cggaaacaac ccttccgcta ttccagatgc atctttcttc cgatttcaaa
     3061 tctcctgcac tctccgccag aagtatctga tatgcgttct gtgatacacc gcgaaggggg
     3121 gaagagagta tccagctgaa aaccggatcc ggaccgcttc cgccgggatc ggaaagatta
     3181 ttgcatctca gttcctgaac tgtcaaattc ctgccggcat ccctgctgca ggacaacagg
     3241 gcaaatgcca tgaatattac cggaattgcc tttttcattg taggtctgtt atcggattat
     3301 gtatatatac tgctttatga tcttctgccg gaaataaatt ccggttccga aataaaggta
     3361 ataatcccgt atgacattta tctcaaaaag ccatatttat tcagctggtg agccgggaat
     3421 cccgcagtgt catctgcggg cttcgggcaa aggcctgatc cccgggcgca caaaaaaggg
     3481 gcaatgataa tatatcatgc cccggattat tcaactaagg taaggtttat tttttcagca
     3541 ggtcgcgtat ttctgtcagg agtttttctt cttttgtcgg ctcgggagga ggagcagggg
     3601 cttctagctc tttcttcttg agcgtattca ttcccttaat aagcatgaac actgcaaatg
     3661 ctatgatcag aaaatcaatt gtcacctgta tgaaatttcc ataattccag gtaacggccg
     3721 gttcaagatc cgtggcagcc ttcattacca gtttcaggtc ggtgaaatta accccgccca
     3781 ggagtaatcc gatagggggc atgattatgt cgttcaccac ggaggaaacg attttaccga
     3841 atgcagcacc aatgattata ccgacagcca tgtcgacaac attgccccgc attgcaaatg
     3901 ctttgaattc atcaattagt ttcatagtta cgggtttaag ttaattgtta aaattaaaat
     3961 ttataagaaa atcctactcc gaatatctcc ttgaactgta ccagtgaacc cactgatctg
     4021 ttctcatcgg caagctttat ctttatgtca tcatcataaa gaaggtgggt gttgaaactt
     4081 acggaactgt atttgtttat tttcaggccg
//
LOCUS       DAOSPX010000301         4108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19601_length_4108_cov_4.469282, whole genome shotgun sequence.
ACCESSION   DAOSPX010000301 DAOSPX010000000
VERSION     DAOSPX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19601_length_4108_cov_4.469282"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLZ75_12070"
     CDS             <1..239
                     /locus_tag="PLZ75_12070"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOS73170.1"
                     /translation="FSANAAEAVRPFVQAAELARELGLEINAGHDLNLDNLNYLHRNI
                     PWLKEVSIGHALISDALYLGLENTIQMYKKQLED"
     gene            245..1045
                     /locus_tag="PLZ75_12075"
     CDS             245..1045
                     /locus_tag="PLZ75_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOS73171.1"
                     /translation="MKLYCRKSGKGPPLIILHALYGSSDNWMSVARAISSEYTVYLPD
                     LRNHGLSPHSDIHDYNALSDDIDELARELNLEKFFLAGHSMGGKTALFYARKWPEKLE
                     GLLIADISPFPAKGRRSREYRLHLEILRTINETGLSEISSRSDVENLISGKISSGRIR
                     GFILKNLQRMPDGKFRWKINAPALLRNLDNIMDGLPRPDGSSEPVTGFPVIFLKGEHS
                     DYLPDDDRSDILRIFPSAGFETIRNAGHWLNADNPEAVISAFSALRDH"
     gene            complement(1135..1767)
                     /gene="udk"
                     /locus_tag="PLZ75_12080"
     CDS             complement(1135..1767)
                     /gene="udk"
                     /locus_tag="PLZ75_12080"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOS73172.1"
                     /translation="MLIVGIAGGTGSGKTTVVNKLKKEFPNGEIVVIPQDAYYRDNSH
                     IPPEDRQKINFDHPDSLEFSLLIRHLRELKEGRAVEMPVYSYLTCLRSKETIPVKPAK
                     VIVVEGILILTDSELRDMFDIKVFVDADADDRLGRVIQRDIIERGRSVLMVLERYHDT
                     VKPSHLQFIEPSKRYADIIIPGGGENEVGIRVLVSIIEKHLAEKGENGEK"
     gene            1858..2838
                     /locus_tag="PLZ75_12085"
     CDS             1858..2838
                     /locus_tag="PLZ75_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOS73173.1"
                     /translation="MSELYPLKFEPILKETIWGGNSLVNRYNKKGDPEKTYGESWEIS
                     AVQDNLSVVSNGFLAGNNIEDLIEVYMGDITGDSVFEKFGNEFPLLIKLIEARKDLSI
                     QVHPGNELAKKRHRAYGKTEMWYILESARDARIYSGFRESLSREEYISKLENGKLAEV
                     LNADISSPGDVFFTPAGRIHAIGAGNLLVEIQQTSDITYRIFDWNRTGPDGKPRELHT
                     GLALDAIDFNAAGSNKSSAKPQLNKTENLVNCEFFTTNIISFNESVKKEYSLLDSFVI
                     YICTEGGFLIRQNGNAEEVIKGESVLLPAMINEVILEPLPGATLLEIFIQ"
     gene            2866..3633
                     /locus_tag="PLZ75_12090"
     CDS             2866..3633
                     /locus_tag="PLZ75_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73174.1"
                     /translation="MKAETISAALAVIILAMAGCRGGGAGKEASAASDTISIPDTGYT
                     GIKQYMGGNRIIKEVTFRNGVRHGLTKTYYPGGQLYQTFWYENGLRQDSGRYYYVEGQ
                     LFRTTPYKNDTINGIQKQYYRTGKLKAMIGYDMGMRTPFFEEYDNKGRLIRDYPEIVA
                     AVSDEYNTRSLYRISLELSDKGTRVKFNRGEFTNERFDTAYITPLNTRDGKAVISLKK
                     SGQAGKNYLGVIAEITTPFGNRLLAYKRIDLPYNDLN"
     gene            3643..>4108
                     /gene="yihA"
                     /locus_tag="PLZ75_12095"
     CDS             3643..>4108
                     /gene="yihA"
                     /locus_tag="PLZ75_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOS73175.1"
                     /translation="MIIRSARYISSSLTPDGFPSSHLPEIAFIGRSNVGKSSLINMLT
                     GYGKLAKTSGEPGKTRTINHFLINDSWYLVDLPGYGYARAAAAQRRDWMKITTGYILS
                     RENLLCLFVLIDPRHKPQKSDTGFMEFLGINRIPFARVFTKGDKMSRASLKKS"
BASE COUNT         1200 a          997 c          971 g          940 t
ORIGIN      
        1 ggttttcagc aaatgcggca gaggctgtca gaccattcgt tcaggctgcg gaactggcca
       61 gggaactggg cctggaaata aatgccggcc atgacctgaa tctggataac ctcaactacc
      121 tacacaggaa tatcccctgg ctgaaggagg tttccatcgg ccatgcactg atttcggacg
      181 ccctctatct cggactcgag aacactatac agatgtataa aaagcagctg gaagactgac
      241 ggccatgaag ctttactgcc gcaaatccgg gaagggacct ccgcttatta ttctccatgc
      301 cctgtacggt tcatccgaca actggatgtc agtggccagg gccatcagtt cagaatatac
      361 ggtttatctg cccgacctca ggaatcatgg cctttccccc catagcgata ttcatgacta
      421 caatgccctg agtgatgata ttgatgaact ggcccgggaa ctgaacctgg aaaagttctt
      481 ccttgccggc cacagcatgg gaggcaagac tgcactgttc tatgccagga aatggccgga
      541 aaaactggaa ggactgctga tagccgacat ttccccgttt cctgccaaag gcagaagatc
      601 aagggaatac aggctgcacc ttgaaatcct caggacaatc aacgaaaccg gtttgtcgga
      661 aatttcttcc cgcagtgatg tggaaaatct catttccggg aagatctcct ccggaaggat
      721 aagaggattc atattgaaaa atcttcagag gatgcccgac ggaaagttca ggtggaaaat
      781 caatgcccct gccctcctgc ggaatctgga caatataatg gacggtctgc caaggccgga
      841 tggcagcagt gaacccgtca ccggattccc cgtgattttc ctgaaagggg aacactcaga
      901 ctatctgccc gatgatgacc gaagtgacat tctcaggatc ttcccctctg ccgggtttga
      961 aaccatcaga aatgcaggcc actggctgaa tgctgacaat ccggaagcag tcatttcagc
     1021 tttttcggca ctccgtgatc actgatgaat tacttctgaa tgttattccg ggcttaccgc
     1081 ctcctgacca tcaccgatca aggaccgctg gcatatcatt caattcttcc cgtatcattt
     1141 ttcaccgttt tcaccttttt cagcaagatg tttttcaata atggaaacaa gcaccctgat
     1201 gccaacctca ttctctccac cgcctggaat aattatgtcg gcatagcgct tggaaggctc
     1261 aataaactga agatgtgatg gttttaccgt atcgtgatat ctctccagca ccatgagcac
     1321 cgatctcccg cgttcaatga tatccctctg aataacacgc ccgagcctgt catccgcatc
     1381 ggcatccaca aaaaccttga tatcaaacat gtctctcaac tcagaatcgg taaggatcag
     1441 gatcccctcc accactatta cctttgccgg cttaacagga atggtctcct tcgacctcag
     1501 gcatgtaaga taggaataaa ccggcatttc aaccgctctt ccctccttca gctccctgag
     1561 atgcctgatg aggagagaaa actcaagcga gtcgggatgg tcgaaattta ttttctgcct
     1621 gtcttcaggg ggaatatggc tgttatccct gtaataggca tcctggggaa tcaccacaat
     1681 ctctccgttg ggaaattcct tcttcagttt gtttactacc gttgtttttc ctgaaccggt
     1741 acctcctgct ataccaacaa tcagcattta ttcgtaaatt agcatcgctc aatccagcca
     1801 aagttaataa aaagtgactg aaatgagacg aagctgaaac ttaaaccaat attcaaaatg
     1861 tcagaactat atcccctaaa gtttgaaccg attctgaaag agaccatctg gggcggcaat
     1921 tcactggtaa accgttacaa taaaaaggga gatcctgaaa aaacatacgg ggagagctgg
     1981 gaaatttctg cggttcagga caatctgtcg gtggtgagca atggtttcct tgccgggaat
     2041 aatattgaag atctcataga agtgtacatg ggagatatta ccggtgattc cgtgtttgaa
     2101 aagttcggaa acgagttccc gctgctcatc aagctgattg aagcaaggaa ggatctttcc
     2161 atacaggttc atccgggcaa tgaacttgca aaaaagagac acagggccta cggaaaaaca
     2221 gaaatgtggt acatactgga aagtgcccgg gatgccagga tctattcggg attcagggaa
     2281 agcctcagcc gggaagaata catcagcaag cttgaaaacg gaaaactggc agaagtgctg
     2341 aacgcagaca tatcatcgcc gggggacgtg ttttttacgc ctgccggaag aatacatgcc
     2401 atcggtgcgg gcaatctgct ggttgagata cagcagactt cggatatcac ctacaggata
     2461 ttcgactgga acagaacggg accggacgga aaaccaaggg agcttcatac cggcctggca
     2521 ctggatgcca tagacttcaa tgctgcagga agcaataaat catcagcaaa accgcagctc
     2581 aacaaaacgg agaatcttgt aaactgcgag ttttttacca caaacattat cagtttcaat
     2641 gaatctgtaa aaaaggaata cagcctcctt gattccttcg tgatttatat ctgcaccgaa
     2701 ggcggattcc tcatcaggca gaacggaaat gcggaagagg taataaaagg agaaagcgtt
     2761 ctcctcccgg caatgattaa cgaagtaata ctggagccgc ttcccggagc cactcttctg
     2821 gagattttca tacagtaaaa tacacttacc caaatatcgc ataaaatgaa agccgagaca
     2881 atatcagcag cattggcagt aattatcctg gcaatggcag gatgcagggg cggaggagcg
     2941 ggaaaagaag catccgccgc cagtgacaca atcagcatcc cggatacggg atacacggga
     3001 attaaacagt atatgggcgg aaaccggatc atcaaggaag ttaccttcag gaatggtgtc
     3061 cggcacggat taacgaaaac atattatccg ggaggtcagc tttaccagac tttctggtat
     3121 gagaacggcc tgagacagga ctccggacgt tattattacg ttgaaggaca gctgttcaga
     3181 acaaccccgt acaaaaatga cactattaac gggattcaga agcagtacta cagaaccggg
     3241 aaactgaagg ccatgatagg atatgacatg ggtatgcgca ctcccttctt tgaggaatat
     3301 gacaacaaag gcaggctgat acgggattat cccgaaattg tcgcagctgt aagtgatgaa
     3361 tacaataccc ggagcctgta caggatatcc ctggaactgt cagacaaggg cacaagggta
     3421 aaattcaaca ggggcgagtt caccaacgaa cgctttgaca ccgcatatat cacaccgcta
     3481 aacaccaggg acggaaaggc tgtgatcagc ctgaagaaaa gcggacaggc ggggaaaaat
     3541 tacctgggcg ttattgcaga aataaccacg cccttcggaa acaggctgct tgcttataaa
     3601 aggattgatc tgccctataa tgatcttaac taggctgctg aaatgataat ccgttcagcc
     3661 cgatatatca gcagcagttt aacaccagac gggtttccgt cttcacatct tcccgaaata
     3721 gcgtttatag ggcgctcaaa tgtcggcaaa tcctcgctta ttaatatgct tacaggatac
     3781 ggaaaactgg caaagacctc gggtgaaccc ggcaaaacca ggaccatcaa tcactttctc
     3841 attaatgaca gctggtatct cgttgacctg cccggctatg gctacgccag ggcagcggct
     3901 gcccaaagac gggactggat gaagatcacg accggttata tcctcagcag ggaaaacctt
     3961 ttatgcctgt tcgtactgat tgatccgaga cacaaacccc agaaaagcga taccggtttc
     4021 atggagtttc tcggaatcaa ccgcattccc tttgccaggg tctttacaaa gggagacaaa
     4081 atgagcaggg ccagcctgaa gaagtcca
//
LOCUS       DAOSPX010000302         4100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19654_length_4100_cov_3.534487, whole genome shotgun sequence.
ACCESSION   DAOSPX010000302 DAOSPX010000000
VERSION     DAOSPX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19654_length_4100_cov_3.534487"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2630)
                     /gene="purL"
                     /locus_tag="PLZ75_12100"
                     /gene_synonym="purI"
     CDS             complement(<1..2630)
                     /gene="purL"
                     /locus_tag="PLZ75_12100"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOS73176.1"
                     /translation="MILFFKTNTGRIIATETTEKLSEDDLSKLEWLFGNAEFTGKKSV
                     KGRFRGPRKDLVSPWSTNAVEITRLIGIEGIRRIEELEPLNDLQKWDPMLQSVYEDPD
                     QRIFTVGKSPDPVRYIDNIADYNKTEGLALSDDETGYLGDLSLKIGRLLTDSEIFAFS
                     QINSEHCRHKIFNGTFIIDGGKMEKTLFELIKLTTKTNPNRVVSAYRDNCAFLEGPLV
                     EQFAPRTHDRPDYYGIRSYKSVLSLKAETHNFPTTVEPWNGGSTGTGGEIRDRMAGGR
                     GAFPIAGTAVYMTSYPRIDDKSRPWEEKIKERPWLYQTPADILIKASDGAGNFGNKFG
                     QPLICGSVLTFEHSENHRQYGFDKVVMMAGGIGTGKKTGSLKGTPAKGDLIILLGGDN
                     YRIGMGGGAVSSVDTGKYDSSIELNAVQRANPEMQKRVYNAIRALAETDNNPVISIHD
                     HGAGGHLNCLSELVEDTGGIIDTGKLPVGDPTLSEKEIIGNESQERMALVLKRKSVET
                     LRNIAERERAPVYIIGEVTGDKQFTLYNSLTGEKPVDLKIDWLLGSPLKTIMKDNSES
                     QSFEKVRYKAENLREYLGMVLSIEEVACKDWLTNKVDRSVTGLVARQQCAGPLQLPLN
                     DLGAISLDYTGKSGMAVSVGHAPGAGLIDPEAGSVLSVAEALTNIIWAPLINGLRSIS
                     LSANWMWPSRNKGEDARLYKAVKAASDFAISLGINIPTGKDSLSMTQKYTDRVVYSPG
                     TVIISATGEVKNINRIAGPVVMNDPYTSLLYLDMSSDSLKTGGSSLAQVLNMLGDEAP
                     TVRDAEYFALVFDTVQDLIMNDLIISGHDISAGGIIITLLEMCFSNNEGGLVVDLSAI
                     GEPDTVKLLFSQNPGIVIQVR"
     gene            complement(2703..>4100)
                     /locus_tag="PLZ75_12105"
     CDS             complement(2703..>4100)
                     /locus_tag="PLZ75_12105"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOS73177.1"
                     /translation="NSHRMLASISVIPVPGKNELKAYTDTVIAVNDLSRAILAREKPR
                     PGAGAKPVPDRIGEPSVIRHVLYIIKENRTYDQILGDMKEGRGDPTLCTYGENVTPNT
                     HKLCREFLLLDNFHVSGKCSAEGHQWTDASIVTDYIEKNMNAWFRSYPHVQADALVYA
                     PSGFIWDNGMKHGKKVRIYGEASSPVFNDPDVSWTDIYQKYINGEKVDFTNHTTLEPV
                     KDILCPDYPSYGPHVFTDIMRADVFIRELREFEAMEGDQLPELMIMALPNDHTAGTRP
                     GYPTPRAMVADNDLALGRIVEAVSKSKFWANTAILVVEDDSQGGWDHVSAYRTVAMVI
                     SPYSRLRKTISTYYTQPSMIRTIEQILGLPPMNIQDAIANPMADCFTDKADLTPYTAL
                     PSNIPLDEMNKPLSELSGRALHYAKKSMLPVFDNVDTGHDDLLNRILWHDARGSTPYP
                     AKYAGAVGEADEDDD"
BASE COUNT          939 a         1018 c          910 g         1233 t
ORIGIN      
        1 cgcacctgaa tgacaatacc gggattctgg ctgaaaagaa gctttaccgt atccggttca
       61 cctattgcag aaagatcgac aaccagtcct ccttcattgt tggaaaaaca catttcaaga
      121 agagtgatga ttatcccgcc ggcagagata tcatgtcccg aaatgatcag atcattcatt
      181 atcagatcct gaaccgtgtc aaacacaagt gcaaaatatt ctgcatctct taccgtgggt
      241 gcttcatcgc ccagcatgtt cagtacctgt gcaagactgc tccctccggt tttgagtgaa
      301 tcgctgctca tatccagata gagcaggctg gtatatggat cgttcatcac aacaggcccg
      361 gcaatcctgt ttatgttttt tacctctccc gttgccgaga ttattactgt tcccggtgaa
      421 tataccaccc ggtcagtgta tttctgagtc atcgacaggc tgtccttgcc tgtgggaatg
      481 ttaatgccca gcgatatcgc aaagtcactt gcagctttta ctgccttgta aagcctggcg
      541 tcttcacctt tgtttctgga aggccacatc cagttggcag agagggaaat gcttctcagt
      601 ccgttgataa gcggtgccca gattatattg gtcagtgctt cggcaaccga aaggaccgaa
      661 cctgcttccg gatctatcag tccggctccg ggcgcatgcc ctaccgaaac agccatccct
      721 gattttcctg tgtagtcaag agaaatggcg ccgaggtcgt tgagcggcag ctgcagggga
      781 ccagcacatt gctgccttgc caccaggccg gtaaccgacc tgtcaacctt gtttgtgagc
      841 cagtccttgc aggcaacttc ttcaatactc agtaccatgc cgagatattc cctcagattt
      901 tctgccttgt acctgacttt ctcaaaggat tggctctctg aattgtcttt cattatagtt
      961 ttcagagggc tgcccagtag ccagtcaatt tttaaatcca caggtttttc acctgtaagg
     1021 gaattgtaaa gagtaaactg tttatctcct gttacttcac ctataatata aaccggcgct
     1081 ctttctcttt cagctatatt acggagtgtt tcaaccgatt tccttttcag tacaagggcc
     1141 attcgttcct gtgattcatt accgataatc tctttttccg agagtgtcgg atcgcccacg
     1201 ggcagctttc ccgtgtcaat aatccctcct gtgtcttcca ccagttcgga cagacagtta
     1261 agatgacctc cggccccgtg gtcatgtatg gaaataaccg gattgttgtc agtttcagcc
     1321 agtgccctta ttgcattgta cactcttttc tgcatttcag gattggcacg ctgcacggca
     1381 ttcagctcta tagaactgtc atatttgccg gtatccactg aagatacggc acctccgccc
     1441 atccctatcc tgtagttatc tcctccaaga agaatgatga gatcaccttt tgcaggagtg
     1501 cccttgaggc tgccggtttt cttgcctgtt cctattccgc ccgccatcat gacaacctta
     1561 tcaaagccgt attgcctgtg attttccgag tgctcgaaag ttagcaccga accgcagata
     1621 agaggctgac cgaatttgtt accgaaattg ccggctccgt cggatgcctt tatcagtata
     1681 tctgcgggtg tctgatacaa ccagggacgt tcctttattt tttcttccca gggacggctt
     1741 ttatcatcaa tcctgggata tgatgtcata tagacagcgg ttccggctat cggaaaggca
     1801 ccccttcccc cggccatacg gtcgcggatc tcgccgcccg tgcctgttga gcctccgttc
     1861 cagggctcaa ccgttgtagg gaaattatgt gtttcagctt ttaacgacaa tactgatttg
     1921 tagctcctga tgccgtaata atcgggcctg tcatgtgtcc ggggagcaaa ctgttcaaca
     1981 agaggtcctt ctaggaaggc acaattgtcc ctgtaggcag atacaacccg gttgggattt
     2041 gtttttgttg tcagtttgat aagctcaaag agggtttttt ccattttccc gccatcaata
     2101 ataaaggtgc cgttaaaaat tttgtgccgg caatgttctg aattgatctg agaaaatgca
     2161 aaaatctcgc tgtcagtcag taatcttccg attttaaggc taagatcccc gaggtatccg
     2221 gtttcgtcat cacttaaggc cagaccttca gttttattgt aatcagcgat attgtcgata
     2281 tatctgacag gatcggggct tttgccaaca gtaaaaatac gctgatccgg atcttcatag
     2341 actgactgaa gcataggatc ccatttctgc aggtcgttta aaggctccag ttcctctatt
     2401 ctccttatcc cctcaatgcc tattaacctg gtgatttcca cggcattggt gctccaggga
     2461 gagaccaggt ccttcctggg tcccctgaac cgtcccttaa cactcttctt cccggtaaat
     2521 tcggcattcc cgaaaagcca ttccagtttt gagagatcat cttccgacag tttttccgtt
     2581 gtctcagttg caattatcct gcccgtattt gttttgaaaa aaaggatcat gttttaatta
     2641 ttaataattc aatatttccg gaaagaacga cattgtgaca gggtttgctt ttccggtacc
     2701 gtttaatcat cgtcttcatc tgcctctcca actgctcctg catactttgc cggataggga
     2761 gtgctgcccc ttgcatcgtg ccatagtatt ctgttcagga ggtcatcatg tcccgtatcc
     2821 acattgtcaa atacgggaag catgctcttt tttgcatagt gcaatgccct tcccgataat
     2881 tctgacaaag gcttgttcat ttcgtcgagt ggtatgttgc taggaagagc ggtatagggt
     2941 gtaagatctg ccttgtctgt gaaacagtcg gccatgggat tggctattgc atcctggata
     3001 ttcatcgggg gcaatccaag gatctgttca attgtcctta tcatggaagg ttgtgtataa
     3061 tatgtgctga tggttttcct aagccttgaa tagggactta ttaccatggc aactgtccgg
     3121 taggctgata catggtccca gcccccctgt gaatcatctt ccactacaag gattgctgtg
     3181 tttgcccaga atttactttt gcttacggct tcaacaattc ttcccagcgc aaggtcatta
     3241 tccgcaacca ttgccctggg agtggggtat ccgggtctgg taccggcggt atgatcgttg
     3301 ggcagagcca ttatcatcag ttccggaagc tggtctcctt ccatggcttc aaattccctg
     3361 agttctctga tgaacacatc tgccctcatg atatccgtga acacatgcgg accgtatgag
     3421 ggataatcag gacacagaat atccttaacc ggttccaggg ttgtatgatt tgtaaaatca
     3481 actttttcac cgtttatata cttctggtaa atatctgtcc agcttacatc cgggtcattg
     3541 aaaacaggtg aagaggcttc gccgtatatt cttacctttt ttccgtgttt cattccgttg
     3601 tcccagataa agcctgaagg tgcgtagaca agagcatcag cctgcacatg cggatagctc
     3661 ctgaaccagg cattcatatt cttttcaata taatcggtta ctattgatgc gtcggtccac
     3721 tgatgtcctt ctgcagaaca tttgccagat acatgaaaat tgtcgagcag caggaattcc
     3781 ctgcacagct tatgggtatt cggagttaca ttttctccgt aggtacacag ggtaggatct
     3841 cctctgcctt ccttcatgtc accaaggatc tggtcgtatg ttctgttttc cttgatgatg
     3901 taaagaacat gcctgattac ggatggttct ccaatcctgt cgggtacagg cttcgcgccg
     3961 gctccgggcc tgggtttttc ccttgcaagt attgctctag aaagatcgtt tactgcaatt
     4021 acagtatctg tatatgcctt aagctcattc ttgcccggaa ccggtataac tgatattgat
     4081 gcaagcatgc ggtgggaatt
//
LOCUS       DAOSPX010000303         4086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19746_length_4086_cov_3.839990, whole genome shotgun sequence.
ACCESSION   DAOSPX010000303 DAOSPX010000000
VERSION     DAOSPX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19746_length_4086_cov_3.839990"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PLZ75_12110"
     CDS             <1..878
                     /locus_tag="PLZ75_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOS73178.1"
                     /translation="AHMIYDDEENTILALKKFLSYLPSNNVENPPLVPDDGSVQDVCE
                     KLRDIVPDDPNKAYDVKDVIQLVADRNSFFETAELFAPNLVTGFARMKGRTVGIVANQ
                     PKVLAGVLDIDSSVKGARFIRFCDAFNIPILTFEDVPGFLPGADQEHNGIIRHGAKML
                     FAYSEATVPKITVILRKAYGGAFIVMNSKNLGGDFSFAWPTAEIAVMGPDGAVAILYR
                     KELAEAKDPAGLKKELTRKYRDKVANPFIADELGYIDEVIDPAVTRKKILSALNALGN
                     KWVKVPERKHGNIPL"
     gene            989..2494
                     /locus_tag="PLZ75_12115"
     CDS             989..2494
                     /locus_tag="PLZ75_12115"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOS73179.1"
                     /translation="MLIRKILIANRGEIAVRIMKSARKMNIPTVAVKTAAEPDAMYLS
                     FADEIIDNTDDTSEIPVFLDIEKLISIALSTGANALHPGYGFLAENAYFAQKCIENGI
                     IFIGPSPDAIYKMGNKTIARKLAMQNKIPLAMGSEGNIADEEEAVVFAEKIGYPVIIK
                     AASGGGGRGMRIVRDENEMVKMFPVARREAEKAFKDPSVFIEKYIENPKHIEFQVLGD
                     TRGNIIHLYERECSIQRKHQKLLEEAPSSALDDKLREEMGNMAVRIAKAVNYHSAGTV
                     EFLLDSDRNFYFMEMNTRIQVEHPVTELITGIDLVEQQIRIANGEALQFEQKDIRING
                     WAIECRINAEDVQSGFSPSPGRIEKMSMPDYPCVRVDTGVREGSAIVANYDSMIAKLI
                     ISGKDRKEVITRCKMALDKVWIKGTKTTLPFFRLLVRNQKFQEGNYTTAFVEKDLEKY
                     YYNSEYEEMLAAWLATKMFIEENLQADSINIDFSEGKEMSPWLLNKRINQF"
     gene            2508..3035
                     /locus_tag="PLZ75_12120"
     CDS             2508..3035
                     /locus_tag="PLZ75_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOS73180.1"
                     /translation="MNARLQINKLFALSSENRKFSFSVPLKNTIRIGKRDYLVQLKTD
                     EKYGTYILWKNRRYPVEIVRSRQNKYEILFNDISYVFTVETPFSLKRMKVINTGRGKS
                     GHESVKAPMPGKLIDILVREGAPILRGEPLVILEAMKMQNEILSPVNGTVLKIHARPN
                     TNVMKDDVLVEIKAE"
     gene            complement(3061..>4086)
                     /gene="pepD"
                     /locus_tag="PLZ75_12125"
     CDS             complement(3061..>4086)
                     /gene="pepD"
                     /locus_tag="PLZ75_12125"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HOS73181.1"
                     /translation="EETGMTGVFGLKKDLLKGDILLNLDSEDENELCVGCAGGMDISA
                     SLEYREEETPSGMTAYRIEAKGLKGGHSGVDIALGRANSNKLMFRFLMQAESDFGIRL
                     AEAGGGNLRNAIPRESYSVVLVQTGKTGDFEQFTRDYEKMYRDEFTDTEPDLVFSFEK
                     CGMPARVINAEDQYRIIRAAFACPNGVQRMSQAVKDLVETSNNMAIVKCAGGNFEVHN
                     LCRSSVDSSKEATAWRIAAVFQLIGAKISMSGGYPGWKPNMNSPILGTITDIYSRMFG
                     EKPEIKAIHAGLECAIIGGTYPELDMISFGPTIRHPHSPDEKVNIPSVKKFYDLLCKT
                     LEEIPLK"
BASE COUNT         1203 a          899 c          967 g         1017 t
ORIGIN      
        1 tggcacatat gatttatgat gatgaggaaa acactatact ggcccttaag aaattccttt
       61 cctaccttcc atcaaacaat gtggaaaacc ctccccttgt tcccgacgac gggtcagtgc
      121 aggatgtatg tgaaaagctc agggatattg ttcccgacga tcccaacaag gcttatgacg
      181 ttaaggatgt aatacagctt gtggctgaca gaaacagctt tttcgagacc gcggaactgt
      241 tcgcaccgaa ccttgttact ggatttgcga gaatgaaggg aagaactgtc ggcatagttg
      301 ctaatcagcc caaagttctg gccggtgtcc tggatatcga ctcctcggtg aaaggtgcca
      361 gattcataag gttctgcgat gctttcaaca ttcccattct tactttcgag gatgtacccg
      421 gctttcttcc cggcgcagat caggaacata acggaataat aaggcatggt gcaaaaatgt
      481 tgtttgcata cagtgaagca acggtaccga aaataaccgt cattctcagg aaagcctacg
      541 ggggtgcctt tatcgtgatg aacagcaaaa atctgggagg agatttcagc tttgcctggc
      601 ccactgcgga aatagctgtc atgggaccgg acggggctgt ggcaatcctt tacaggaagg
      661 agctcgcgga agcaaaggat cctgctggcc tcaagaagga acttacaaga aaataccgcg
      721 acaaggtggc aaatcctttc attgccgatg agctgggtta tatcgatgaa gttatcgatc
      781 cggctgttac gaggaaaaaa atactttcgg cactgaatgc cctgggtaac aaatgggtaa
      841 aagtgcctga aaggaagcac ggcaatattc ccctgtaaag aatcattgac aaaatgattc
      901 agtgacaggc aatattccgg aaaaatacat atacggttta atgcggattg cagctgcaaa
      961 tccgccggtt taaaaaaagt atgataaaat gttgatacgt aagattctta ttgcaaacag
     1021 gggtgaaata gccgtaagga taatgaagag tgcccggaaa atgaatattc ccaccgttgc
     1081 agttaaaact gcagctgaac ctgatgcaat gtatctttca tttgccgatg agattattga
     1141 caacacggat gatacttcag aaattcccgt ttttctggat attgagaagt tgatctcaat
     1201 agccctcagc acaggggcca atgcccttca tccgggctac gggtttcttg cagaaaatgc
     1261 atattttgcc cagaaatgca ttgaaaacgg aataatcttt atcggcccct ctcctgatgc
     1321 aatttacaaa atggggaata aaaccatagc caggaagctt gcaatgcaga ataaaattcc
     1381 cctggcaatg ggaagtgaag ggaacatagc agatgaggag gaagcagttg ttttcgctga
     1441 aaagatcggt tatcctgtca taataaaggc agcatcgggt ggcggcggac ggggaatgag
     1501 gattgtcagg gatgagaacg aaatggttaa aatgtttccg gttgcaagac gggaagcaga
     1561 aaaagcattc aaggaccctt cggtattcat tgaaaaatat atagaaaatc ccaaacacat
     1621 agaatttcag gtactcgggg acaccagggg caacataatc catctctacg aaagggaatg
     1681 ctccattcag cggaaacatc agaaacttct tgaagaggct ccctcttccg ctctcgatga
     1741 caagctgagg gaggagatgg ggaatatggc tgtcagaata gcaaaagcag tcaattacca
     1801 ttctgccggt actgttgaat tccttcttga ctcggacagg aatttctatt tcatggaaat
     1861 gaataccagg atacaggtgg agcatcccgt taccgaactt ataaccggta ttgacctggt
     1921 tgagcagcag atccgcatag caaacggtga agccctgcag tttgaacaga aagacatccg
     1981 gattaacgga tgggctattg aatgcaggat caatgccgaa gatgttcagt caggtttttc
     2041 cccgtcaccg gggcggatag aaaagatgag catgcccgat tatccctgtg tgagggttga
     2101 taccggtgta agggagggat ccgccattgt agccaactac gactccatga tagcaaagct
     2161 catcatcagc ggaaaagacc ggaaggaagt aataacgaga tgtaaaatgg ctcttgacaa
     2221 ggtatggata aaaggaacaa aaacaaccct tccgtttttc aggctgttgg tcaggaacca
     2281 gaagttccag gagggaaact acacaaccgc ctttgttgaa aaggacctgg aaaaatatta
     2341 ttacaacagc gaatatgaag aaatgctggc tgcctggctt gcgaccaaaa tgttcatcga
     2401 agaaaatctc caggcagaca gtattaatat cgatttcagc gaagggaagg aaatgagccc
     2461 ctggctgctg aataaaagga taaaccagtt ttaaacagac tgcagcaatg aatgccagat
     2521 tacagataaa caaacttttt gcactctcat cggaaaacag gaaattcagt ttcagtgtac
     2581 ctttaaagaa tacgatccgt attggcaaaa gggattatct ggttcagctg aagacggacg
     2641 aaaagtacgg tacatatatt ctgtggaaga acaggcgcta tccggtagag atagtcagat
     2701 cgcggcagaa taaatatgaa atattattca atgacatcag ctatgttttt actgtagaaa
     2761 caccattctc actcaagaga atgaaagtga tcaataccgg aaggggaaaa agcgggcatg
     2821 aatccgtgaa ggctcccatg ccgggcaagc tgattgatat acttgtcaga gagggagctc
     2881 ccatactccg gggtgaacct cttgtaatcc ttgaagcgat gaaaatgcag aacgagattc
     2941 tctcgccagt aaacggcaca gttctgaaaa tccacgccag gcccaataca aatgtaatga
     3001 aggacgatgt actggtggag atcaaggcgg aatagacctg attgaacaat cagcccggct
     3061 ttatttcagc gggatttcct ccagtgtttt acaaagcaga tcataaaatt tcttcactga
     3121 aggtatattc accttttcat caggtgaatg aggatgtctg attgtcggtc cgaaagagat
     3181 catatccagt tccggatatg ttcccccgat aattgcacat tcaagtccgg catgaatggc
     3241 cttgatttca ggtttttcgc caaacatcct gctgtatatg tcagtaatgg tcccgagtat
     3301 gggcgaattc atatttggtt tccatcccgg atagccgccc gacatactta tctttgcacc
     3361 gattagctgg aaaacggccg ctatcctcca ggctgtggcc tcttttgaag aatcaacaga
     3421 gctcctgcag aggttatgaa cctcaaaatt cccgccggca cattttacaa ttgccatatt
     3481 gttggatgtc tcaaccagat ccttcactgc ctggctcatt ctctgaactc cattcggaca
     3541 tgcaaacgcg gcccggataa tcctgtactg gtcctctgcg tttatcaccc gtgcgggcat
     3601 tccgcatttt tcaaaggaaa acacaagatc aggttccgtg tcagtgaact catccctgta
     3661 catcttttca taatctcttg tgaattgctc gaaatctccg gtttttccgg tctgtaccag
     3721 gactaccgaa taagattcac gaggtattgc attccggaga ttgccgccgc cggcttcggc
     3781 aagtctgatt ccgaaatctg actctgcctg catcagaaag cggaacatca gtttgtttga
     3841 attggcccgg ccaagtgcga tatccactcc tgagtgtccg cctttaaggc cctttgcctc
     3901 aattctgtag gcagtcattc cggaaggtgt ctcttcctcc ctgtattcca gtgaggcact
     3961 gatatccatt cccccagcgc atccgacgca aagttcattt tcatcctctg aatcaagatt
     4021 aagcaggatg tctcccttaa gaagatcttt tttcagtccg aacacacccg tcatgccggt
     4081 ttcttc
//
LOCUS       DAOSPX010000304         4073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_19825_length_4073_cov_3.392982, whole genome shotgun sequence.
ACCESSION   DAOSPX010000304 DAOSPX010000000
VERSION     DAOSPX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19825_length_4073_cov_3.392982"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PLZ75_12130"
     CDS             complement(<1..1322)
                     /locus_tag="PLZ75_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS73182.1"
                     /translation="MDRREFSKKVVTGSAAMGMSPLILTGPGWKGANDRVNVAVLGIR
                     AQGQEHIREYQKLNNVNVAALCDPDSNLFEERVKKYYTDRGLPKPKLYTDLRKLYEDR
                     DIDAVSVVTPNHWHALASIWALQAGKHVSVEKPCCHNFFEGMKLVEAAEKYKLIVQDG
                     AEQRSNPCALSMAEFLHSGRLGEVYMAKGLCYKWRDTIGKTADEAVPSGVDYDLWLGP
                     APKRPFSINRFHYNWHWNWDYGNGDMGNQGVHEMDIARWGLNVTLPTRITAAGGHFMF
                     DDDQNTPNVLIAVFEFPGPGGGGDKKKILQFEVRHWITNREGIRSDNPAEDAYMVSAD
                     NVVGNLFYGSKGFMTKNVNEWQVYYGKERKEGERGSGLGNHYQAFIDAIRANDQKLAR
                     GDIREGFYSCALVHLGNISYRLGRSLEFDPVKMKFINAPDADALLTRE"
     gene            1513..3618
                     /locus_tag="PLZ75_12135"
     CDS             1513..3618
                     /locus_tag="PLZ75_12135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HOS73183.1"
                     /translation="MIQNRIKHSLSAVFFVFVSAALFSQKVSVEILKTEDAAVSGWQI
                     LDGQYRKVISGNDFSRGDSLLFSLEAEKRYLLAISLSDNLVRDTTLYLLMISGEPVLR
                     INSGLEPGDHFFPFFTGVKKEAGKIVGGTDADIADFPWQVYYEAGQYICGGSIISEDW
                     VITAAHCTRNKDGTAVPLNEMFVKAGATNPYQAQSGKKYLISEVIVHENFNNRTLDND
                     LALLRLQHPVDVVNAKAVRLLTAADALEGATDPGVMTWVTGWGLTQVFPEEFPWVLQK
                     VQLPIVSKEQASVVWKNISDNVVMAGYRNADRDACNGDSGGPLVVSVSGEYRLAGITS
                     WGSEDCDTYSAYTRISAYEDWIRTNTGISEYIPPVPAGGAIICPGTVSSEYSVNPVSG
                     AQQYQWELYPADAGSISFSAASATVEWDQDYLGPASIKLRVVINNRLSEWSRLNVEVA
                     PATRIITQPGDRTLCAGQNIDLGITAEGHNLEYHRYKNGILTNIFADGDYFIAGALTD
                     DSGIYRCMVTGSCGTAYTNDISIAVHPLTRIKSFSRDMTIDSGDDVKLEVDASGHELS
                     YQWIKDRENLAGSNEPDLWLTNANSSDIGLYQVMVSGSCGTELSGRAYVYVRQEDSAP
                     DPGVIVWPTITDGVINAALDSDESYNIRIFSYTGRIIRELNNCRYNTVIYMNNQPSGI
                     YIINIYNRHFRWSQKFIRK"
     gene            3663..3935
                     /locus_tag="PLZ75_12140"
     CDS             3663..3935
                     /locus_tag="PLZ75_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOS73184.1"
                     /translation="MGNKILYRIRVYGFVQGVGFRWNAAREARRIGIAGHVRNMPDGS
                     VYIEAEGSREQLEKFTEWCREGPGYVESVETESSVPVNYRDFKIIH"
BASE COUNT         1045 a          953 c         1032 g         1043 t
ORIGIN      
        1 tattccctgg tgagcagggc atccgcatcg ggtgcgttta tgaatttcat tttcaccgga
       61 tcaaattcaa gggaacgccc caggcggtat gaaatattcc caagatgtac cagggcacat
      121 gaatagaatc cttcccttat atcacccctg gccagtttct ggtcattggc ccggattgca
      181 tcaataaatg cctggtaatg gttccccagt ccgcttcccc tttctccctc tttacgttcc
      241 ttgccgtaat agacttgcca ttcattcaca ttttttgtca tgaatccttt cgaaccgtag
      301 aaaagatttc ccacaacatt gtctgcactc accatatagg cgtcctcagc cggattgtca
      361 ctcctgatcc cttcccggtt ggttatccag tggcgcactt cgaactgaag aatcttcttt
      421 ttgtcgcccc cgccgccagg accgggaaat tcaaacactg cgatcagcac attgggcgtg
      481 ttctggtcat cgtcaaacat gaaatgccct cctgcggcgg ttatcctggt gggcagcgtt
      541 acattgagcc cccagcgtgc aatatccatt tcatggaccc cctggttgcc catgtctccg
      601 ttgccgtaat cccagttcca gtgccagttg taatggaacc tgtttatgga gaaaggcctt
      661 tttggtgcag gaccgagcca gagatcataa tcaacgccgg aaggaaccgc ctcatcagct
      721 gtcttcccta ttgtgtcgcg ccatttatag caaagaccct ttgccatgta tacctcgccg
      781 agcctgccgg aatgtaggaa ttcagccatt gacagcgcac agggattgct cctctgttct
      841 gccccgtcct gcactatcag cttatatttc tccgcggcct caacaagctt catcccttca
      901 aagaaattat ggcagcaggg tttttcaacc gagacatgct ttccggcctg caaggcccag
      961 attgatgcca gtgcatgcca gtgatttgga gtgactaccg acactgcatc aatatccctg
     1021 tcctcgtaca acttccgcag gtcggtgtaa agcttcggtt tcgggagtcc cctgtcggta
     1081 tagtacttct ttaccctttc ttcaaaaagg ttgctgtcgg ggtcacacag tgccgcaacg
     1141 ttaacattgt tcagcttctg gtattcccgg atgtgttcct gtccctgtgc ccttatgccg
     1201 agaaccgcaa cattgacccg gtcattggca cccttccatc ccgggccggt gagaataagg
     1261 ggagacatcc ccattgctgc cgatccggtc acgacttttt tactgaattc acgacgatcc
     1321 atatcaatga tttaatgatt ttttgttaaa tataattttt aaaaatgaac ctctgatcac
     1381 agttcagcaa taaattgttg caggtatttg gaatataaaa taaaggattg cattatactt
     1441 gcaggataat tgccggataa taatctgaat ggcacacgct ttttggatat attgccgcta
     1501 actggaaagg aaatgattca gaaccggata aagcacagcc tttcagccgt attttttgtt
     1561 tttgtatcag cagcactgtt ttcccagaag gtgtctgttg aaatccttaa aacagaagat
     1621 gcagctgttt ccggatggca gatactggat ggccagtaca gaaaggtgat ttcaggaaat
     1681 gatttcagcc ggggtgattc attgctcttc tctcttgaag cagaaaagcg atatctcctg
     1741 gcaatcagtc tgtcagacaa ccttgttcgg gacacgacgc tttatctgct gatgatcagc
     1801 ggggagccgg tactcagaat aaactccggc ctggagccgg gtgatcattt cttccccttt
     1861 tttaccggag tcaagaaaga agccggtaag atagtggggg gaaccgatgc cgacattgca
     1921 gatttcccct ggcaggttta ttacgaagca gggcagtata tatgcggagg atcaatcatt
     1981 tcggaagact gggtgatcac tgcggcacat tgtacacgca acaaggacgg aacagcggtt
     2041 cctctgaatg aaatgttcgt caaggccggg gcaaccaacc cctatcaggc gcagtccgga
     2101 aaaaaatatc ttatcagcga agtgattgtc catgagaatt tcaataaccg gaccctggat
     2161 aatgatcttg cattgctcag gcttcagcac ccggttgatg tggttaatgc aaaagcagta
     2221 aggctgctca ctgctgctga tgcccttgaa ggagcaaccg atccgggtgt gatgacatgg
     2281 gttacgggtt ggggactgac acaggtgttt cccgaagagt tcccctgggt tctgcaaaag
     2341 gtgcagctgc cgatagtgtc aaaagaacag gcttccgtgg tctggaaaaa catatccgac
     2401 aatgtagtga tggcgggata ccggaatgca gacagggatg catgcaacgg tgacagcgga
     2461 gggccgctgg tggtcagcgt atccggagaa taccggctgg ccgggataac aagctgggga
     2521 agcgaggact gcgataccta cagtgcctat accaggattt cagcctatga agactggatc
     2581 aggacaaaca ccggtatcag tgaatatatc cctccggttc ccgcaggtgg ggcgataata
     2641 tgtcccggga ctgtttccag tgaatacagt gttaatccgg tttccggagc ccagcagtat
     2701 cagtgggagc tctatcccgc ggatgccggt tcaatcagct tctctgccgc ttcagcaaca
     2761 gttgaatggg atcaggatta tctcggcccg gcaagtatca agctgagggt agtaattaat
     2821 aacaggcttt cggaatggtc gcggctgaat gtggaagttg cgcctgcaac acggattatc
     2881 acccagccgg gcgacagaac cttatgtgcg ggacaaaaca ttgacctagg cattaccgcg
     2941 gaaggacaca atcttgaata tcaccgttac aagaacggaa ttctgacaaa catctttgct
     3001 gacggggatt atttcatagc aggtgccctg accgacgatt ccggcattta ccgttgcatg
     3061 gtgacaggat cctgcggtac ggcctataca aatgatatca gtattgccgt tcatccgctg
     3121 acccgtataa aatccttttc gcgggatatg acaattgatt ccggggatga tgtgaaactt
     3181 gaggttgatg catcgggaca tgaattaagc tatcagtgga ttaaggacag ggagaacctg
     3241 gcgggcagca atgagccgga tctctggctg acgaatgcaa actcatcaga tattggtctt
     3301 tatcaggtta tggtgtcagg ttcgtgcggt acagaactca gcggcagggc atatgtttat
     3361 gtaaggcagg aagattccgc gccggatccc ggtgtgattg tctggcccac cattacggat
     3421 ggtgtcatta acgcagccct tgattcggat gagtcgtaca atatccgcat tttcagctat
     3481 accggaagga taataaggga actgaacaat tgccgttaca atactgtcat ttacatgaac
     3541 aatcaacctt cgggtatcta tatcataaac atatacaaca ggcatttcag atggagtcag
     3601 aagttcatca ggaaataaaa ttgttgtaca tttatcaggc cgttgataat tgaacttctc
     3661 tgatggggaa taagatactg tacaggatcc gggtatatgg atttgtccag ggagttggtt
     3721 tcaggtggaa tgccgcaagg gaagccagga gaattggtat tgccgggcat gtgcggaata
     3781 tgcctgacgg cagtgtgtat attgaagcgg aaggcagcag ggaacagctt gagaagttca
     3841 cggaatggtg cagggagggg cccggctatg ttgaatctgt tgaaacggaa tcatccgtcc
     3901 ccgtcaatta cagggatttt aaaataatac attgataaac cggtaaaata aatctaaaac
     3961 acatcatgaa aacagacagg agaacattta tcaggacagg aactatggcg ctggcaggca
     4021 ctgcactgtt gggcagtaag gcttttgccg cacccaaagg gaagggaatg gtt
//
LOCUS       DAOSPX010000305         4005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_20257_length_4005_cov_4.353671, whole genome shotgun sequence.
ACCESSION   DAOSPX010000305 DAOSPX010000000
VERSION     DAOSPX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20257_length_4005_cov_4.353671"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="PLZ75_12145"
     CDS             <1..1572
                     /locus_tag="PLZ75_12145"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOS73185.1"
                     /translation="KELKYNLDFLGSREFRGRETPSPELEIATLYLGNWAGYTGLKPA
                     MKDGSFYQEVPLMVTAVFQPGTRISLSQGTSDRIYYFGRDFCGSFARSGSYRGEVVFA
                     GMGISNPQSGWDDLADMDLRDKVVVILDAQLPGTVFPLGFTMTNRLSSRINAIRGRGA
                     AAVLTVVDPERQKMKEEGQKIFDYIPTGRLQIMYDSRRTDFSSADGSQADRGQMPPSQ
                     NSRPPLPFERAEISHDLAAQMLGITREKIAGMFSAVAKGEKVSSFEVEGVSVKLDVEV
                     EYYPAVSRNVVAMVEGSDPVLRNEYVVICGHHDGRGIDDGGIIAGADDNGSACVALME
                     IGKALLAERPKRSVLLVWFTGEEQMMNGSHYFVNNCPVPLEKITACLNMDMISRNDPD
                     SLFLVGSDLLSSELDASIKKVNRNYDLDFTFDYLYSNLTHPQQVYFRSDHYPFVRFGI
                     PSVWFFCGFTYDYHTVRDVPERVDYHKLYRTTRLVYLTTMEVGNMKSLLKLDVNPAVT
                     SRGAHNLREPSLFGR"
     gene            1629..3413
                     /locus_tag="PLZ75_12150"
     CDS             1629..3413
                     /locus_tag="PLZ75_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73186.1"
                     /translation="MKYIFPLLLFNFLVCVAEAQHLPGFRNSESFDEQQLVIEDAENG
                     IRILINAPLEGFGKKDKVLLVFYALPNGSSIEHTFGRKAEPGLDYRYDIQHIGAQTRF
                     LRNVIGDRTIVVAYLENSRLSWPAWVANTTDPQEKIRKIVDCVTGIFVRWEPEIALNG
                     HSGGGRFIFSYIDSLEEIPPSIVRIGFLDSTYGYKDTLHGKKIASWLMSGRKRYLCTL
                     AYNDSVVVYNGKPLVSPTGGTWYRSKMMKNYLSGYFRFREKERDSLLWSTSPGRRIVF
                     ILKPNPERKIYHTTQVELNGFIHSILSGTKHEQKDYTYFGKRAYSDLIDDSLALPIRM
                     LNIPVRDPLSESGSEFMNRIRSLSPGEREEEIYKAVSSGNIPDFLRDPVTMSGEFMDS
                     RGKAHRVVYEVMPDYISVGSNEDFCRIPMNPYTAQKVADDFGASLITSVISDNIHRSA
                     DIRLEPFYYAPVGHENELVEKFAEHNRQIEKQLSESGGRQGDLVSGIKKDVILSSRID
                     AQPGKVVIYGWHRPDGKPIQPVYSGHIYWYVDYSHGIRLINNQVLIDGKPKLFTEILQ
                     DPVLFRIFSDEDTPMKQPAYRKPGQDGD"
     gene            complement(3472..3933)
                     /locus_tag="PLZ75_12155"
     CDS             complement(3472..3933)
                     /locus_tag="PLZ75_12155"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOS73187.1"
                     /translation="MKTERRSVLKKLFAAVIGVSGAGLATRAKASVPMEKETPGAVNF
                     QDQPLFSPFTKYGNLVFVSGHGQRLEGDIKVHTDNVLKMIEAQLIKAGSSMEKVLKVT
                     VFLETMDDYHGMNEAYKGRFGKNPPARTTVAVPNGLPPADNLIQMDCIAYV"
BASE COUNT         1086 a          869 c         1036 g         1014 t
ORIGIN      
        1 aaggagttga agtacaatct cgatttcctt ggttcaaggg agttccgggg aagagagacg
       61 ccttcgccgg aacttgagat agcaaccctg taccttggca actgggccgg atacacaggc
      121 cttaaaccgg caatgaagga cggctccttc tatcaggagg tgcccctgat ggtgacagca
      181 gtctttcagc ccggaacacg cataagcctt tcacagggca catccgaccg catatattat
      241 tttggaaggg acttctgcgg cagcttcgca cgaagcggct cctacagggg agaagtggtc
      301 tttgccggga tgggcataag caatcctcag tccggatggg atgaccttgc agatatggat
      361 cttcgggata aggttgtggt gatacttgat gcacagcttc cggggacggt ttttccactt
      421 ggatttacca tgaccaacag gctctcgtca cggataaatg ccatccgcgg aagaggtgcc
      481 gcggcagtgc tgactgttgt tgatccggag aggcagaaga tgaaggagga gggacagaag
      541 attttcgatt atatccccac cggcaggctg cagattatgt atgactcgcg caggacggat
      601 ttcagctctg cggacggatc gcaggcagat cgcggacaga tgcccccgtc acagaactcc
      661 cgtccgcccc ttcctttcga gagggctgag ataagtcacg acctggccgc acaaatgctt
      721 ggcataacca gggaaaagat agccggtatg ttctcagcag tggcaaaagg ggagaaggta
      781 tcttcatttg aagttgaagg ggtcagcgta aagctcgacg tggaagtgga atattacccg
      841 gctgtctccc gcaatgttgt tgcaatggtt gaagggtccg atccggtgct gaggaatgaa
      901 tatgtagtta tctgcgggca tcatgacgga cggggcatcg acgacggtgg aatcattgcc
      961 ggtgccgatg acaacggcag cgcatgcgtc gccctgatgg agataggcaa ggcactcctg
     1021 gcagagaggc cgaaaaggtc ggtcctcctc gtgtggttca ccggcgagga gcagatgatg
     1081 aacggttcac attatttcgt gaataactgt ccggtgccgt tggagaagat cactgcatgc
     1141 ctcaacatgg acatgatcag tcgtaatgat cccgacagcc ttttcctggt gggttctgac
     1201 ctgctgagct cggagcttga tgcatcaata aaaaaggtga accgcaacta tgatcttgac
     1261 ttcacgtttg actacctcta ctccaacctt acacacccgc agcaggttta cttcaggagc
     1321 gaccactacc cgtttgtcag gtttggcatt ccgtcagtat ggtttttctg cgggtttacc
     1381 tatgattacc ataccgtcag ggacgtgcct gagcgggtcg attatcacaa gctttacagg
     1441 acgacaaggc ttgtttacct gacaaccatg gaagttggca acatgaagag tctcctgaag
     1501 cttgacgtga accccgccgt tacttcgcgc ggtgcgcata acctcaggga acccagtctg
     1561 ttcggcaggt aatggcggaa gggttttttg gaggcctgtg ataaaaagat aatttttaca
     1621 aaagaaagat gaagtacatc tttcccttac tgctgtttaa ttttctggtc tgcgtcgcgg
     1681 aggcacagca cctgccggga ttcagaaatt cggaaagctt tgatgaacaa cagcttgtga
     1741 ttgaagatgc ggaaaatggc atccgtattc ttataaatgc tcctctggaa ggtttcggta
     1801 aaaaggataa ggtactgctt gttttttatg ctttaccaaa cggcagttca attgaacata
     1861 cctttgggag gaaagcggaa ccgggacttg attatcgcta tgatattcaa catataggag
     1921 cacagacaag gttcctcaga aatgtgatcg gagaccggac gattgttgta gcttatcttg
     1981 aaaattcacg tttaagctgg cctgcatggg tggcaaatac aactgatccc caggaaaaga
     2041 taaggaaaat tgttgactgt gtaaccggga tctttgtccg ctgggaacct gaaatagcgc
     2101 tgaatggtca cagcggaggc ggaagattta tattcagcta tattgattcc ctggaagaga
     2161 tccccccttc aattgtcagg ataggcttcc tcgacagtac ctacggctat aaagatacgc
     2221 ttcacggaaa gaaaatagca agctggctga tgtcgggaag aaaaagatac ctgtgcactc
     2281 ttgcttataa cgacagcgtg gttgtctata atgggaaacc tcttgtttca ccgacaggag
     2341 gaacctggta caggagtaaa atgatgaaga attatctttc cggctatttc cgcttcaggg
     2401 agaaggaaag agattcactg ctatggagta catcaccggg cagacggata gtgttcatcc
     2461 tgaaaccaaa tccggaaaga aaaatttacc atacaaccca ggtggagcta aacggattta
     2521 ttcacagtat cctcagcgga acgaaacatg aacaaaagga ttatacttat tttgggaaaa
     2581 gggcatattc agatctgata gatgattctc ttgcattacc tatcagaatg ctcaatattc
     2641 ctgttcgtga cccattatcg gaaagcggat ctgaattcat gaacaggatc agatcattat
     2701 cgcccgggga acgtgaggag gagatttaca aagctgtttc atcgggtaat ataccggatt
     2761 ttttaagaga tccggtgaca atgagcggag aattcatgga ttcccggggt aaagcccaca
     2821 gggttgttta tgaggttatg ccggattata tttcggttgg cagcaatgag gatttttgcc
     2881 gtataccaat gaatccttat accgcccaaa aagtggccga tgactttgga gcttctttga
     2941 tcacatctgt aataagcgac aatattcaca gatcagcaga tatcagactt gaaccttttt
     3001 attatgcccc ggtaggccat gaaaatgaat tagtcgaaaa atttgctgag cataacaggc
     3061 agattgaaaa acagctttca gaatcgggag gcaggcaggg ggatctggta tcaggtatca
     3121 aaaaggatgt aatactttca tcccggatcg atgctcaacc cgggaaggta gtgatctacg
     3181 gctggcacag gccggatggc aagccgatac aaccggtata cagtggtcat atttactggt
     3241 atgtcgatta tagtcatggg ataagactta ttaacaacca ggtacttata gatggcaagc
     3301 caaaattgtt cactgaaata ttacaggacc cggtactatt caggatcttt agcgatgagg
     3361 atactccgat gaagcaacct gcgtacagaa aaccgggtca ggacggggat tgaatactgt
     3421 tgaaaacagg ataaggcaag ccgtttttcc gggattttcg ctcagtacgg attaaacata
     3481 agcaatacaa tccatctgaa tcagattgtc ggcgggtggc aatccgtttg gcacggcgac
     3541 tgttgtccgg gccgggggat tctttccgaa acgtccctta taggcttcgt tcattccatg
     3601 ataatcatcc atggtttcca ggaataccgt caccttaagg accttttcca tggaagagcc
     3661 ggctttgatg agctgggctt ctatcatttt aagaacattg tcggtatgca ccttaatatc
     3721 accctcaaga cgttgtccgt gacctgaaac aaatacaaga tttccgtatt tagtaaaagg
     3781 cgagaataag ggctgatcct gaaaattaac cgcaccggga gtttcctttt ccatgggcac
     3841 acttgccttt gcccttgttg ccaggcctgc accgcttacg ccaataactg ctgcaaagag
     3901 tttctttagt actgatcttc tttctgtttt cattttgata tagttttagt tttgagaata
     3961 taacagcctg tcccgcaaag cgggagaaac gcatattaat gaaga
//
LOCUS       DAOSPX010000306         3988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_20364_length_3988_cov_3.745487, whole genome shotgun sequence.
ACCESSION   DAOSPX010000306 DAOSPX010000000
VERSION     DAOSPX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20364_length_3988_cov_3.745487"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2503
                     /locus_tag="PLZ75_12160"
     CDS             <1..2503
                     /locus_tag="PLZ75_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOS73188.1"
                     /translation="SAGVAGGRGNIWDSGTRKSKTNLAVYEGRSLEPFTKYYWSVRIR
                     DGSGRLSKISETASFETGMMEAAAWKGSWISDSRDINLKPAACFRKSFRIPGRIKSAR
                     AYIAAAGLYELTVNGVRAGDHLLDPAYTRFDRRNLYVTHDITELLREGENAAGVLMGN
                     GWYNHQSTAVWFFHEAPWRARPSFCMEIHISLEDGSSMIIPSGTDWKTATGPVIFNSI
                     YTAEHYDARQEKPGWDLPGYDDSYWKEPVLVPAPSQNITAQAMHPIRAEAELSAKRLN
                     RINSNTWVYDFGRNIAGVTRLRISGSRGTVIRLKHGERLMPDGRVDQSNIDVHYRPAD
                     DSDPFQTDIFILAGRGEEVFMPRFNYKGFQYVELTSDRPVQVNRESLSAFFMHSDVPP
                     AGYIKSSDTLLNRIWEAANNSYLSNLFGYPTDCPQREKNGWTGDAHIAVETGLYNFDA
                     VTVYEKWLNDHLDEQQPNGVLPAIIPTSGWGYHWANGPDWTSTIAIIPWNIYLFYGDP
                     HLLNLCYDNIKRYVDHITELSPDGLTDWGLGDWIPVKSVSPKELTSSIYYFVDACILA
                     KAARLFGNDADHEKYSRLAQKIKDAVNEKYLDREKGIYGSGFQTEMSFPLMWGIVPDE
                     FREVIASNLAESVRANDYHIDVGLHGTKSILNALSENGYPDIAFRLAVQDTFPSWGWW
                     IVNGATTMFENWPINPESDISMNHIMFGEISAWMYKALGGIFPDEDQPGFKNVILKPH
                     FVDGLDKFEARHDGPYGTIISSWEKAGDRVVYRITLPPGSTATFYPGEGKILEQDREV
                     NKNRHIVQRNTEDGRALLLGSGKYEFTIQKHKAGH"
     gene            complement(2500..>3988)
                     /locus_tag="PLZ75_12165"
     CDS             complement(2500..>3988)
                     /locus_tag="PLZ75_12165"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOS73189.1"
                     /translation="VTGYWLYRETKEAWILEAVESIQANSRDWTSYYEEFPWDSSANA
                     EKRIPLNWKEDGLTAHVVNNAMAIKYPGLWYQQSKNERYRNAVFDGIMKYDLHHGQAG
                     GRFSGDEHLSGKSPVRGTELCSVVEYMFSLEELYAVFGDNSLADRLESLAFNSLPGTT
                     TADMWAHQYDQQSNQVLVTGDPRDWSTNGNYSNIYGLMPNFACCLANMHQGWPKFAAS
                     LWMATNDNGLAAVAYSPCTVSARVGKGDKVTIREETDYPFRGKILLTISTDKNVRFPL
                     YLRIPGWADDVSISYKGRSAEAEAGSSLRIDEKWKNGDEIIIDIPMKLRLERRYNNSV
                     SLLRGPLFFSLGIDRDYRSTRLNYDNFSYKGSVDWEIYPQSPWNYALLADENSLPRGF
                     SVIENSIEKYPFADRGDMIWSPDSGRYIEWEKDPPVVISARGIRIPEWTMKNNSADIP
                     PVSPVKTSLNPEIIRLVPYGSAKLRITEFPVADPSYVRETMRPGN"
BASE COUNT         1049 a          914 c         1010 g         1015 t
ORIGIN      
        1 ttctgcgggt gtggcaggag gcaggggaaa tatttgggac tccggaacca ggaaatccaa
       61 aaccaacctg gctgtttacg aaggccggag tctggaacca tttacaaagt attactggag
      121 tgtaaggata agggatggaa gcggaagatt gtcaaaaata tctgaaaccg catcctttga
      181 aacagggatg atggaagcgg ctgcctggaa gggcagctgg ataagtgatt caagggatat
      241 taatctgaag cctgcagcct gtttcaggaa atcattccgt attccgggca ggatcaagtc
      301 ggcccgtgca tatatagctg cagccggact gtatgaactg acagttaacg gagtcagggc
      361 gggcgatcat ttgctggatc ccgcatatac acgctttgac cggcggaatc tgtatgttac
      421 ccacgatata actgaactgc tcagggaagg ggaaaacgct gcaggggtac ttatgggaaa
      481 cggctggtat aaccaccagt caacggcggt atggtttttc cacgaagctc cgtggcgtgc
      541 aagaccctct ttttgcatgg agatccacat atccctggag gacggatcat ccatgatcat
      601 tcccagcgga accgactgga aaactgctac cggcccggtg atcttcaata gtatttatac
      661 agccgaacat tatgacgcaa ggcaggaaaa gcccggttgg gatctgcccg gatatgatga
      721 ctcatactgg aaagaaccgg tactggtgcc ggcaccatca cagaatatca cggcacaggc
      781 catgcacccc ataagagctg aagctgaatt gtccgcaaag agacttaaca ggataaacag
      841 caatacatgg gtttatgatt tcggacggaa tattgccggt gtcacccgtt tgaggataag
      901 cggaagccgc ggtacggtga tcagactgaa gcacggggaa agattaatgc cggacggacg
      961 tgttgaccag tctaatattg acgttcatta ccgcccggcg gatgacagtg atcccttcca
     1021 gacagatata ttcattctgg ccggaagggg ggaggaggtc tttatgcccc ggtttaacta
     1081 caaaggcttt cagtatgttg aactgacatc tgacaggccg gttcaggtga acagggaaag
     1141 tctttctgca ttcttcatgc acagcgatgt tcctccggca ggatacatta aatcctcaga
     1201 tacattgctg aataggatct gggaggctgc aaacaattcc tatctctcca accttttcgg
     1261 ttatccgact gattgtcccc agagggagaa aaacgggtgg acaggagatg cgcatattgc
     1321 cgttgaaacg ggcctgtata attttgatgc tgttacagta tatgaaaaat ggctgaacga
     1381 ccatctggat gaacagcagc ccaatggtgt gctgccggca attattccca ccagcggctg
     1441 gggctatcac tgggcaaacg gacctgactg gaccagcacc atagccatta taccatggaa
     1501 tatatacttg ttttacggcg atccgcactt gctgaaccta tgttatgaca acataaaaag
     1561 atatgttgac catataacgg aactgagccc ggacggactt accgactggg ggcttggtga
     1621 ctggataccg gttaagtcgg tatcacctaa ggaattaact tcttcgattt attattttgt
     1681 ggatgcatgc attcttgcaa aggctgccag gctgtttgga aatgatgctg atcatgagaa
     1741 atacagcagg ctggctcaga aaataaagga tgctgtaaat gagaaatacc tggacaggga
     1801 aaaggggatt tacggaagcg gatttcagac tgaaatgagc tttccgctta tgtggggtat
     1861 tgtacccgat gagttccggg aagtcattgc ttcgaacctg gcagaaagtg taagggcaaa
     1921 cgattatcat attgatgtgg gccttcatgg cacaaaatcc attctgaatg ccctcagtga
     1981 aaacggatat cccgatattg ccttcagact ggcagttcag gatactttcc cttcctgggg
     2041 ctggtggatt gtaaatggcg caacaaccat gtttgaaaac tggccgatca atcctgaatc
     2101 ggatatctca atgaaccaca tcatgtttgg tgagataagc gcatggatgt acaaggcact
     2161 gggaggcata tttccagatg aggatcagcc cggctttaaa aatgtaatac tgaagccgca
     2221 ctttgttgac ggtcttgata agtttgaggc acggcatgac ggaccatacg gcacaatcat
     2281 atcatcgtgg gaaaaggcgg gagacagggt cgtttacagg atcaccctgc ctccgggaag
     2341 tacggcaaca ttttatcctg gagagggcaa aatcctggag caggacaggg aagtaaataa
     2401 aaacaggcac atcgttcaga ggaatactga ggatggccgg gcacttttac ttggctcggg
     2461 gaaatatgag tttacaatac aaaagcataa ggcagggcat tagttacccg gcctcattgt
     2521 ttccctgaca tacgaagggt cggccacagg gaactcagtg atccttaatt ttgctgagcc
     2581 atagggcaca agcctgataa tttccgggtt aagactggtt tttaccggac tcaccggagg
     2641 gatatcagct gaattgttct tcattgtcca ttccggtatt cttatgcctc tggcacttat
     2701 cacaaccggg ggatcctttt cccattcaat atatctgcct gaatcaggcg accagatcat
     2761 atcacccctg tccgcgaagg ggtatttttc aatactgttt tcaatgacgc tgaatcccct
     2821 gggcagagaa ttttcatcag ccagaagggc atagttccac ggactttgcg gatatatttc
     2881 ccagtcaaca ctgcccttgt agctgaaatt atcgtaattc agtcttgtgc ttctgtaatc
     2941 cctgtcaatt ccaagcgaaa agaagagcgg acctctcagg agggaaacag aattattgta
     3001 tcttctttcc agtcttaatt tcatgggaat atcgataata atctcatcac cgttcttcca
     3061 tttttcatca atccgcaggg atgatccggc ctcagcttca gctgaccgtc ccttataact
     3121 tattgataca tcgtccgccc atcccggaat tctcagataa agcgggaacc ttacattttt
     3181 atcagtgctg atggtcagta agatttttcc cctgaaggga tagtctgttt cttccctgat
     3241 cgtgacttta tctccttttc cgaccctggc gcttacagtg cacggactgt aggctactgc
     3301 tgcaaggcca ttgtcgtttg tagccatcca gaggcttgct gcaaatttag gccatccctg
     3361 gtgcatgttt gccaggcagc aggcgaaatt gggcataaga ccataaatat ttgaataatt
     3421 gccgtttgta gaccagtctc tcggatctcc cgtaacaaga acctggttcg actgctggtc
     3481 gtactgatgg gcccacatgt ctgcagttgt tgttccggga agtgaattga aggccagtga
     3541 ttccagccgg tctgccagac tgttgtcacc aaagaccgca tacagctcct ccagggagaa
     3601 catgtattca accacggaac agagctctgt tcctcggacg gggctctttc cggaaagatg
     3661 ttcatcgcct gaaaaccttc ccccggcctg gccgtgatga aggtcatatt tcataatgcc
     3721 gtcaaaaacg gcattcctgt atctttcatt ctttgactgc tgataccaga gaccgggata
     3781 cttgattgcc atagcattgt tgaccacatg tgccgtcagg ccgtcttcct tccagttgag
     3841 cggaatacgt ttctcggcat tggctgaaga gtcccaggga aattcttcat aataggaagt
     3901 ccagtcgcgg ctgttggcct gtattgattc aacggcttcc agaatccagg cttccttagt
     3961 ttcgcggtaa agccagtatc ctgtaact
//
LOCUS       DAOSPX010000307         3970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_20484_length_3970_cov_4.095530, whole genome shotgun sequence.
ACCESSION   DAOSPX010000307 DAOSPX010000000
VERSION     DAOSPX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20484_length_3970_cov_4.095530"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PLZ75_12170"
     CDS             <1..1174
                     /locus_tag="PLZ75_12170"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOS73190.1"
                     /translation="TAQDLRQDEDVLDTWFSSWLWPITSFDGINQPDNPDLKYYYPTN
                     DLITAPEILFFWVARMIIAGFEYQGEKPFHNVYLTGLVRDQQRRKMSKSLGNSPEPLD
                     LIAKFGADGVRVGMLLCSPAGNDLLYDDSLPEQGRNFANKIWNAFRLVKSWTVGADEA
                     QPASSALAVKWMREAIGQGIAQIDSDFRRFRISEALMTVYKLFWDDFSGWYLEIVKPE
                     YGKPVDRASYDASISFFDTLLRLIHPFMPFISEEIWQLLDERKDGESIMVSRMPEPGK
                     FSRELISGFETVKETVTAIRSLRKERNIPSGEKIMLFARPAGTFAGEEFFPVIIRLCN
                     LADLAKVDSRQEDAVSFMVGTTEFYVPLGGKIDIEGEIARIREDLRYHRGFLESVM"
     gene            1178..1327
                     /locus_tag="PLZ75_12175"
     CDS             1178..1327
                     /locus_tag="PLZ75_12175"
                     /inference="COORDINATES: protein motif:HMM:NF021941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73191.1"
                     /translation="MNNERFVQNAPSPVLDLERKKKSDAEQKIKSLEERLRGLGDQGE
                     SLVTK"
     gene            complement(1300..2604)
                     /locus_tag="PLZ75_12180"
     CDS             complement(1300..2604)
                     /locus_tag="PLZ75_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73192.1"
                     /translation="MKYSWILSIVVILLSGCRAEEKDIYLTPDKALSYFEKIRESCDS
                     DNGRLWGTNLYGPMMFIDRTTRKMVANLPDKDGLLREKDGVYTGLYPRDQLISNTAVR
                     YGSTLFGIAPLPGEEDEFRIITRALHCLFHRYQDSIGFTSSGYNTANLDEKNARLYLK
                     LEWKALRKAIETEDDGHCFAIRDALIFRGANHELYPAFVRDQIRFESYEGLATFTHLM
                     LVTSSPEEFREKLLDLLDRFYSFPSYSRSYGLIHGALYASLLQRKGFDFRTIRSEDTD
                     LGEQVRQLYDIRLPAVCRDIAGSLAFNYDLEEIKAEEIEREKEINQRIDRVVSTFTDK
                     PVVYVELISPYFDFEPEDIRPLAAHGTLYEKIRISDNWGKLTVDKGGCLVSANLKYLR
                     VTARGFEEGRNRIEGEGWHMIINNDWEILQVGDNYFVTRLSP"
     gene            complement(2701..3543)
                     /gene="nadC"
                     /locus_tag="PLZ75_12185"
     CDS             complement(2701..3543)
                     /gene="nadC"
                     /locus_tag="PLZ75_12185"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOS73193.1"
                     /translation="MEDNLLREFLIRCIEEDVGDGDHSSLSCIPAGSVGKAKLLIKEE
                     GILAGVRIALELFRIIDPGLRCEVLKQDGDRISFGDTGFILHGNLHSILKSERLMLNI
                     MQRMSGIATSTGRYVDRIAGTGARILDTRKTTPGMRMLEKEAVRIGGGMNHRMGLYDM
                     IMLKDNHIAYAGSIETAIDKTREYLERNGRELKIVIEAQSTDEVKSILKHGGVNRIML
                     DNFSIADTRVAVDMIEGRFETESSGKINLGTVRQYAECGVDFISVGAITHQISSLDIS
                     LTAI"
     gene            complement(3533..3937)
                     /locus_tag="PLZ75_12190"
     CDS             complement(3533..3937)
                     /locus_tag="PLZ75_12190"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOS73194.1"
                     /translation="MDFKNLVSILVLALLCTNIQAQDQQRIPPGISIAIKAGNAAEMA
                     KYMNSTVELLLLGKEDFYKKNVAETILKDFFATYQTKDFIIRHQGAKNEAQYAIGNLK
                     TVKGDFRVYFLLKRVGPELLIHTIRIESEDGR"
BASE COUNT          964 a          954 c          922 g         1130 t
ORIGIN      
        1 gactgcacag gatctccggc aggatgaaga tgtgcttgat acatggttct cctcgtggtt
       61 gtggcccata acatcctttg acggaataaa ccagcctgac aatcctgatc ttaagtatta
      121 ctatccgaca aatgacctca ttaccgcacc ggagatcctt tttttctggg ttgccaggat
      181 gataatcgcc ggttttgaat accagggtga aaagcccttt cacaatgtat atctcactgg
      241 tctggtacgt gaccagcaaa ggaggaaaat gtccaagtcg ctcggcaatt ctcccgaacc
      301 cttggatctg attgcgaagt ttggtgctga cggtgtgagg gttggcatgc ttctttgctc
      361 cccggcagga aatgatttgc tttatgatga ttccctgccc gagcagggca ggaattttgc
      421 caacaagata tggaatgctt tccgcctggt gaaaagctgg actgtaggtg ctgatgaagc
      481 acagcctgca tcctcggccc tggccgtgaa atggatgaga gaagccatag ggcaaggaat
      541 tgctcagatt gattcggatt tccgcagatt caggatatcc gaagccctga tgacggtata
      601 taaactgttc tgggatgatt tttccggatg gtatctggaa atagtgaagc cggagtacgg
      661 gaagccggtg gacagggcca gctatgatgc aagcatttct tttttcgaca ccctgctcag
      721 gctgatccat ccctttatgc cctttatctc ggaggaaata tggcagcttc ttgatgaaag
      781 aaaggatggc gaaagcatca tggtcagccg catgcctgaa ccgggaaaat tcagccggga
      841 gctcatttcc ggatttgaaa cggtaaagga gactgttacg gccatcaggt ccctgaggaa
      901 ggagaggaat attccctccg gggagaagat tatgcttttt gcaagaccgg cgggcacttt
      961 tgccggggag gagttttttc cggtaatcat ccggctctgt aatcttgcgg atctggcaaa
     1021 ggttgattca aggcaggaag atgccgtgtc cttcatggtt ggaaccaccg agttctatgt
     1081 acctctcggc ggcaaaattg atattgaggg tgaaattgcc aggataaggg aagatctcag
     1141 atatcacagg ggctttctcg aatcggtaat gtaaaaactg aacaatgaac gctttgttca
     1201 gaatgcacct tctccggttc tggatcttga acggaaaaag aaatcggatg ccgagcagaa
     1261 gataaaatca cttgaggaaa gactgcgcgg tcttggtgat cagggagaaa gtctggtcac
     1321 aaaataattg tcaccgacct ggaggatttc ccagtcatta ttgattatca tatgccatcc
     1381 ttcgccttca atcctgttcc ggccctcttc aaatcccctg gccgtgacgc gcaggtattt
     1441 caggttggct gataccaggc atcctccctt gtcgaccgtc agctttcccc agttgtcgga
     1501 gatgcggatc ttttcataca gggtgccgtg ggcggcaagt ggtctgatat cttcgggctc
     1561 aaaatcgaaa tacggactga tcagttccac atatacaacc ggcttgtccg taaatgtact
     1621 taccaccctg tctatcctct ggtttatttc cttttccctt tcgatctctt cggcctttat
     1681 ttcctcaaga tcatagttga aggcaaggct tccggctata tccctgcaga cggcaggcag
     1741 cctgatgtca tacaactgtc taacctgttc tcccagatcc gtgtcttccg atcgtattgt
     1801 cctgaagtca aaacccttcc gttgcagcag tgatgcatac agggcgccgt gaatcaatcc
     1861 gtatgacctt gaataggacg gaaaggaata gaacctgtca agaaggtcca ggagcttttc
     1921 cctgaattct tccggtgatg atgtgacaag catcagatgg gtaaaagtag ccagtccctc
     1981 ataactttca aatctgatct ggtctcttac aaaggcagga tagagttcat gatttgctcc
     2041 tctgaagatg agtgcgtccc tgatggcaaa acaatgtccg tcgtcttccg tttctattgc
     2101 cttgcgaagt gctttccatt caagcttcag atacagccgt gcattctttt catccaggtt
     2161 ggctgtgttg tagcctgatg aagtgaaacc tattgaatcc tgatatctgt ggaacaggca
     2221 gtgcagggca cgtgttatta ttctgaattc atcctcctcg ccgggcagcg gggctattcc
     2281 gaacaatgta cttccgtagc ggacagccgt attgcttatt aactgatctc tcgggtagag
     2341 gcctgtgtaa actccgtctt tttccctcag caggccgtcc ttgtcaggta agtttgcaac
     2401 catcttcctg gttgtgcggt caatgaacat cataggtccg taaaggtttg ttccccacag
     2461 cctgccattg tcactgtcgc aggattccct tatcttttca aagtagctga gcgccttgtc
     2521 cggagtcagg taaatatctt tttcctcagc cctgcacccg gataggagaa taaccactat
     2581 cgaaaggatc catgaatatt tcataggaat tttatgagag accaaatgta aataaaaaaa
     2641 cattaatatg taattcagca attattgttc cgcttgagcg ctgccagcgg gaacaggtgt
     2701 ttaaatggcc gtgaggctta tgtcgagact gctgatctgg tgggttatgg caccaacgga
     2761 gataaaatcg acgccacatt cggcatactg tcttaccgtc ccgagattaa tttttccgga
     2821 ggattccgtt tcgaatctgc cttcaatcat gtccactgca acccgggtat ccgcaatact
     2881 gaaattatcg agcattattc tgtttacccc tccgtgcttc agaatacttt tcacttcatc
     2941 cgtactttgg gcctcaatca caattttcag ctccctgccg ttccgttcaa gatattcccg
     3001 ggttttgtca atggctgttt ctatgctgcc agcataggca atatgattgt ctttcagcat
     3061 tatcatatca tacagcccca ttcggtgatt cattcctccc cctattctga cggcttcttt
     3121 ttcaagcatc ctcatacccg gggtggtttt cctggtatca agaatccttg ccccggttcc
     3181 tgcaatccgg tcaacatagc ggccggtact tgttgcaata ccgctcatgc gctgcataat
     3241 attgagcatc agtctttccg atttcagaat ggaatgcagg tttccatgca gaataaagcc
     3301 cgtgtcgcca aagcttattc tgtccccgtc ctgtttcagg acttcacagc ggagaccggg
     3361 atcaatgatt ctgaacagtt caagagcaat ccgcacaccg gcaaggatgc cctcttcctt
     3421 gatgagcagc tttgcctttc caacggaacc ggcaggtatg catgagagtg aagaatggtc
     3481 gccgtcgcca acgtcttctt caatacaacg gattaagaat tcccttagaa gattatcttc
     3541 catcttccga ttctattctg atcgtatgga tcagcaattc cggaccaact cttttcagaa
     3601 ggaaatagac cctgaaatcg cccttcacgg ttttaagatt gcctattgca tactgagctt
     3661 cattctttgc accctgatgc ctgataatga aatcctttgt ctggtaggtg gcaaagaaat
     3721 ccttcaaaat ggtttctgcc acatttttct tgtaaaagtc ctcctttcca agcagaagca
     3781 gttcaaccgt tgagttcatg tattttgcca tttcagctgc attccctgcc tttattgcaa
     3841 tagatatgcc cgggggaatc ctctgctgat cctgtgcctg aatattggtg cataaaaggg
     3901 ccagaacaag tattgaaaca agattcttaa aatccatttg gttacgtttt gctgactttc
     3961 atattcattt
//
LOCUS       DAOSPX010000308         3943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_20673_length_3943_cov_4.866512, whole genome shotgun sequence.
ACCESSION   DAOSPX010000308 DAOSPX010000000
VERSION     DAOSPX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20673_length_3943_cov_4.866512"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /gene="gcvPB"
                     /locus_tag="PLZ75_12195"
     CDS             <1..1550
                     /gene="gcvPB"
                     /locus_tag="PLZ75_12195"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOS73195.1"
                     /translation="KLCSCTIADEKFIRKSSFLEQAAFNSYHSETEMMRYIKMLERRD
                     ISLTHSMISLGSCTMKLNSATSMFALSWPEFANIHPFVPEDQAEGYYQLMDELGEDLK
                     EITGFAAVSFQPNSGAAGEYAGLLVMREYHLSKGEGHRDAVLIPSSAHGTNPASAVMA
                     GMPVVVVECDEGGNIDIDDLRKKTEANRDNLAGFMITYPSTHGVYEPGVVEMVNIIHE
                     AGGLVYMDGANLNAQMGLTSPGRIGADLCHLNLHKTFAIPHGGGGPGMGPILVNEKLA
                     EFLPSHPMVETGGKRGISAVAAAPFGSAHILTISHSFIRMMGSEGLTLAAKYAILNAN
                     YISFALKDDFRTLYKGEEGFVAHEFILDCRNFKSGTGITESDIAKRLMDYGFHAPTLS
                     FPVYGTLMIEPTESESLQELDKFIEAMKSIRREIDEVAEGKADREDNVLKNAPHTVMV
                     ITANEWNHKYDRQKAAFPAGWIVHNKFWPAVGRIDEGYGDRNLVCTCDPIDSYRTADI
                     AFPEIEL"
     gene            1648..2679
                     /locus_tag="PLZ75_12200"
     CDS             1648..2679
                     /locus_tag="PLZ75_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS73196.1"
                     /translation="MKNLFWNDLIRLIDNGKTDYQPVGFIIDSPWIPGWYGISAVDYY
                     SSDEYWLRSNIRAAETFPDVWFLPGFWSEYGMCTEPSAFGSRMIFLDDSLPHAEKILD
                     NIEDVDRLPQPNVKTDGLLPFMIGRLRRNEEAIREADHQIRFAVARGPMNIASFLMGT
                     TEFMMALALDPENSHKLLRKITLFIQDWLSWQAECFSIDGVLILDDIMGFVGEAEFGE
                     FALPYFREIFDHAGTKVRFLHNDADGLITAANLKEMGVNMFNFSFNHSMGEIRELAGP
                     EVVLVGNIPGRDVLAAGTAEQVEEAVKKAFAETGTHERIIWSAGGGMPPDVSDANIHA
                     FIRAVRNNQ"
     gene            2789..>3943
                     /locus_tag="PLZ75_12205"
     CDS             2789..>3943
                     /locus_tag="PLZ75_12205"
                     /inference="COORDINATES: protein motif:HMM:NF027805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOS73197.1"
                     /translation="MKNIILPVLVSVIITSCGGGGAGHKQSKSGKKMDSNYPVSLMTV
                     DPGHFHAALVQRIMYDEVNPEVHVYAPESPDYVMHLNRIRGFNERAENPTSWNEIVYT
                     GPDFFEKMLENRPGNVVVLSGNNLKKAEYISRSVDAGLNVYADKPMIILPEDFPRLEQ
                     AFRTAAEKGVLIYDIMTERFEITTILQKLLSMKEEVFGKLVQGTPDDPAVSKISVHHF
                     LKLVAGAPNIRPGWFFDVDQQGEGIVDVTTHLVDLIQWECFPEQILSPADVSIFSAKR
                     WPTIITREQFKAVTNLEDFPDYLQKDIRDGKLNVFANGEMVYQIRGVWARVSVEWNYE
                     APPGGGDTHYSVMRGSTCDLVIRQGAEEKFIPTLYVENIRGVSPGDFTGTL"
BASE COUNT         1090 a          824 c         1061 g          968 t
ORIGIN      
        1 aaaaactgtg cagctgtaca atagcggatg aaaaattcat caggaagtcc tccttcctgg
       61 agcaggctgc cttcaacagc tatcacagcg aaacggagat gatgcgttat atcaagatgc
      121 ttgaaagaag ggatatttcc cttactcatt caatgatctc tctcggatcc tgtaccatga
      181 agcttaattc cgccacttcg atgtttgcac tcagctggcc cgaatttgca aatattcatc
      241 cctttgtgcc ggaggatcag gctgaggggt attatcagct gatggatgaa ctgggagaag
      301 acctgaaaga gattacagga tttgcagcag tttccttcca gccaaattcc ggtgccgcag
      361 gggaatatgc cggtctcctt gttatgcgtg aatatcattt gtcgaagggg gagggacaca
      421 gggatgctgt tctgatacct tcatcagcac acggtacaaa cccggccagt gcggttatgg
      481 caggcatgcc ggtagtggtt gtcgaatgtg acgagggagg aaacatcgac attgatgacc
      541 tgaggaaaaa gacggaggca aacagggata atctcgcagg gtttatgata acatacccgt
      601 ccacacatgg tgtgtatgaa cccggtgttg ttgaaatggt gaacattatt catgaggccg
      661 gcggactggt atatatggac ggtgcaaacc tgaatgccca gatgggactt accagtcccg
      721 gaagaatagg agccgatctc tgtcatctga acctgcacaa gacatttgca attcctcatg
      781 gaggcggcgg gccgggaatg ggaccgatac ttgtaaatga aaaacttgca gaattccttc
      841 cttcccatcc gatggttgaa accggcggaa agcggggaat aagtgccgtg gcagccgctc
      901 ctttcggaag tgcacatatc ctgaccatat cacattcatt cataaggatg atgggatcgg
      961 aggggctgac actggcagcc aaatacgcga tactgaacgc gaattatatt tcatttgccc
     1021 ttaaggatga tttccggaca ctgtataaag gtgaggaagg ttttgttgcc catgaattca
     1081 tactggactg ccggaatttt aagtccggga cgggtattac cgaatcggat atagccaagc
     1141 gtctgatgga ttacggcttt catgcaccga ccctgtcatt tccggtatac ggtacgctga
     1201 tgattgaacc gacggaatca gagtccctgc aggagctgga taagttcatt gaagccatga
     1261 aatccatccg cagggagatt gatgaagtgg cggaaggaaa ggcagacagg gaagataatg
     1321 ttctcaaaaa cgcaccgcat acggtaatgg ttattactgc aaatgaatgg aatcacaagt
     1381 acgacagaca aaaagctgct ttcccagccg gatggattgt gcacaataag ttctggcccg
     1441 cagtaggaag gatagatgaa ggctatggcg acagaaacct tgtctgtacc tgtgacccca
     1501 tcgattcata cagaacggct gatattgctt tcccggagat tgaactgtag cagaaggcac
     1561 agcctgcaga gacaattaat ttttttccct gaaataaatt acctaattta ttccgggatt
     1621 attttatgaa tcacaagcac cgggcaaatg aaaaatttat tctggaatga cctgatcagg
     1681 ctgattgaca acggaaaaac ggattatcag ccagttggtt ttattattga cagtccctgg
     1741 atacctggat ggtacggcat ctcagccgta gattattatt cctcggacga atactggctc
     1801 cggtccaata tcagggctgc agaaactttc cccgatgtat ggttcctgcc cggattctgg
     1861 tcggaatacg gaatgtgcac cgaaccgtcg gcctttggtt ccaggatgat tttccttgat
     1921 gatagtcttc cccatgccga gaaaatactg gacaatattg aagatgttga caggcttccc
     1981 cagcccaatg taaagaccga cggactgctt ccctttatga ttgggcggct caggaggaat
     2041 gaagaggcaa taagggaggc agatcatcag ataagatttg ccgttgcaag gggaccgatg
     2101 aacatagcca gtttcctgat gggcacaaca gaatttatga tggctcttgc ccttgatccc
     2161 gaaaactctc ataagctgct ccggaaaatc acacttttca tacaggactg gctgagctgg
     2221 caggcggaat gcttctccat tgacggtgtg cttatactgg atgacattat ggggtttgtc
     2281 ggggaggctg agttcgggga gtttgccctg ccctatttca gggagatctt tgatcatgcc
     2341 ggaacgaagg tcaggttcct gcataatgat gctgacggac tcataaccgc tgccaatctg
     2401 aaagagatgg gtgtcaatat gttcaatttc tctttcaatc attcaatggg tgagataagg
     2461 gaacttgccg gaccggaggt ggttctggta gggaatattc ccggaaggga tgtactggcg
     2521 gcaggcacgg cagaacaggt tgaagaagcg gtaaaaaagg cgtttgcgga aacaggaact
     2581 catgaacgga ttatctggtc ggcgggggga ggaatgccgc cggatgtaag tgatgccaac
     2641 attcatgcct tcatcagggc ggtcaggaac aaccagtgaa tattcaatat agtgatagta
     2701 atgcacccca gtgccgttta ttctgccgga gggagctccg gaccggggag ccagaacaga
     2761 aatcttaaaa caacttaata ataaaaaaat gaaaaacata attctgccag tgttggtttc
     2821 agtgattata acttcctgcg gaggcggagg agcaggacat aaacagtcaa aatccggaaa
     2881 aaagatggac agtaattacc cggtaagtct tatgacagtt gatccgggcc attttcatgc
     2941 ggccctggtc cagaggataa tgtatgatga ggtaaatccc gaggtgcatg tatatgcacc
     3001 ggaaagtcct gattatgtca tgcatctcaa caggataagg ggtttcaatg aacgtgccga
     3061 aaaccctact tcctggaatg aaatagtgta tacaggtcct gattttttcg agaagatgct
     3121 ggaaaaccgg cctgggaatg tcgttgttct ttcgggaaac aacctgaaaa aagcggaata
     3181 tatcagcagg tcggttgatg caggactgaa tgtgtatgcc gacaagccga tgataatact
     3241 gcctgaggat tttccccggc ttgaacaggc tttcaggaca gcagcggaga aaggtgtgct
     3301 gatatatgac atcatgactg aaaggtttga gattaccacc atactgcaaa agcttctgtc
     3361 aatgaaggaa gaggtattcg gaaagcttgt acagggaacg cctgatgatc ctgccgtatc
     3421 caagatcagt gtgcatcatt tcctgaagct tgtggcggga gctcccaaca taaggccggg
     3481 atggttcttt gatgtggacc agcaggggga aggaatcgtt gatgttacaa cccatctggt
     3541 ggatcttata cagtgggaat gttttccgga acagatactt tcaccggctg atgtaagcat
     3601 tttttccgca aaacgctggc ctacgattat taccagggag caattcaagg ctgtgacaaa
     3661 ccttgaggat tttcctgact acctgcagaa agatatcagg gacggaaaac tgaatgtgtt
     3721 tgcaaatggt gaaatggtat atcagatcag gggtgtctgg gccagggtgt ctgttgaatg
     3781 gaattatgag gctcctcccg gaggcggaga tactcattat tcggtaatgc ggggcagtac
     3841 ctgcgacctg gtgatcaggc agggggctga agagaaattc attcccaccc tttatgtcga
     3901 gaatatcagg ggagtatccc cgggtgattt caccgggaca ctt
//
LOCUS       DAOSPX010000309         3941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_20692_length_3941_cov_3.815749, whole genome shotgun sequence.
ACCESSION   DAOSPX010000309 DAOSPX010000000
VERSION     DAOSPX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20692_length_3941_cov_3.815749"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PLZ75_12210"
     CDS             complement(<1..130)
                     /locus_tag="PLZ75_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOS73198.1"
                     /translation="MPRTDFKQLLDAGVHFGHLKRKWNPTMAPYIFMERNGIHIIDL"
     gene            complement(244..630)
                     /gene="rpsI"
                     /locus_tag="PLZ75_12215"
     CDS             complement(244..630)
                     /gene="rpsI"
                     /locus_tag="PLZ75_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOS73199.1"
                     /translation="MEPINAIGRRKAAVARVYLSNGRGNITINGKDYKDYFGQATLHY
                     IVTQPLLLLDASDKYDITVNLSGGGVKGQAEALRLGISRALVRIDEENKTILKANGFI
                     TRDPRVVERKKPGRPGARKRFQFSKR"
     gene            complement(640..1092)
                     /gene="rplM"
                     /locus_tag="PLZ75_12220"
     CDS             complement(640..1092)
                     /gene="rplM"
                     /locus_tag="PLZ75_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOS73200.1"
                     /translation="MDTLSYKTVSANRATVSKEWVVIDAEGAALGRLATMIAGLLRGK
                     HKVNFTPHVDCGDNVIVINAEKVSLSGDKWTDKKYVSHTGYPGGQRFTTAATLLKKNP
                     AGLIERAVRGMLPKNRLGRALFRNMYVYSGSSHPHEAQQPRKIEYTNN"
     gene            1274..1747
                     /locus_tag="PLZ75_12225"
     CDS             1274..1747
                     /locus_tag="PLZ75_12225"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009434527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOS73201.1"
                     /translation="MEKDRVFLCRALEIAVQGVNEGGGPFGALIAKDGRIIAESWNNV
                     VLSADPTAHAEINAIRKACEILGTHDLADCILYSSCEPCPMCLGAVYWAGIKKVVYAF
                     DRKEAEKAGFSDKFIYDEIMLEPSSRHVRFLHIPGCCNGPDPFRKWNENENKIPY"
     gene            1719..2261
                     /locus_tag="PLZ75_12230"
     CDS             1719..2261
                     /locus_tag="PLZ75_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOS73202.1"
                     /translation="MKMKIKSPTDMEGEKRLSAEFFSRDVLEVAPELIGKYLVIRRGS
                     VPERYMITETEAYRGTEDKACHASQGETPRNRVMYGRGGRIYVYLIYGMYWMLNFVTG
                     EENNPQAVLIRALEGLTGPGKLTRELGIDRSFYGEDLAVSGRIWVEDGGISPSFRQGP
                     RIGIDYAGEFWKNKPWRYYI"
     gene            2306..>3941
                     /locus_tag="PLZ75_12235"
     CDS             2306..>3941
                     /locus_tag="PLZ75_12235"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOS73203.1"
                     /translation="MNSVLDIFRRWNNIAGWSVFAVSAAVYLMTAEPTVSFWDCGEFI
                     LSAFRLQVGHPPGAPLFLMLGRVATFFAGGDVLKVAFTVNSFSAICSALTILFLFWSV
                     THLVRRVVSRDGELQAKHILPVIGSGAAGALAYTFSDTFWFSAVEGELYALSSLCTAL
                     VFWTMLKWEEEADKPYAGRWIILTAYIIGLSLGIHRLNLLVIPVLVFVVYFKKYEISG
                     KGILNTLLTAILTLGFMVFVLIPGVPKAAGWFELFFVNALGLPYNTGLFVFLAAVTVL
                     LVAGIRYSIRKKNIILNYIITALTVIMIGHSSYAMIMIRSSARPPMNQNNPSDIFALG
                     YYINMEQYGSAPLLFGPYYSAPAIDVKNRVSGYNKVDGKYEPYYRPEYKYDNRFETVF
                     PRMYSRDPEHEQAYTYWAGIRGKKYTITSGSGKRTLVCPTFGENLKFFFRYQAGFMYF
                     RYFMWNFAGRQNDIQGNGNKIHGNWISGFRFIDNARLGSQDQLPSELLENPGRNRYYM
                     LPLLLGIAGILWQYRRDRKGLFLVFLFFFMTGLAIIV"
BASE COUNT          968 a          903 c          964 g         1106 t
ORIGIN      
        1 caagatcaat gatatgaata ccattgcgtt ccataaaaat atacggagcc atggtaggat
       61 tccattttct tttcagatgt ccgaaatgta ctcctgcatc aagtaattgt ttaaaatctg
      121 ttctgggcat gtatcaatat ttaagtttac attctttatt gtcaatcaca gagtagttcc
      181 cttgcgggaa gtctttgcaa tttagatact aaacttccat ctcatccgct aaggcagctg
      241 ttcctagcgt ttgctgaact ggaatctctt ccgggccccg ggcctgccgg gctttttcct
      301 ttcaaccaca cggggatcgc gggttataaa accatttgcc ttgagaatag tcttgttttc
      361 ctcatcaatc ctgaccaatg cccttgatat tccgagccgc agtgcttcag cctgaccctt
      421 taccccgcca cctgaaagat tgacggtaat gtcatacttg tctgaagcat ccagaagaag
      481 cagcggctgg gtaacaatat agtgaagcgt agcctgtccg aaataatcct tgtaatcctt
      541 gccgttaatt gtgatgttgc ccctgccgtt gctcagatat accctggcga cggctgcttt
      601 tcttcttcca atagcattga taggttccat atgagtgagt tagttgtttg tgtattcaat
      661 ttttctgggt tgctgggctt catggggatg ggaagatccg ctatatacat acatgtttct
      721 gaacaatgcc ctgcccaggc ggtttttggg aagcattccc ctgacagccc tttcaatgag
      781 accggcagga ttcttcttta aaagggtagc agctgtggtg aacctttgcc cgccgggata
      841 gccggtatgg cttacatatt ttttatccgt ccatttatcc ccgctcagtg aaaccttctc
      901 agcatttata acaataacat tgtcgccgca gtcaacatga ggtgtaaaat ttaccttgtg
      961 ttttcctctc agcaaacccg ctatcatggt tgccaggcgt ccgagggctg ctccttccgc
     1021 atcaataact acccattcct tgcttacggt tgccctgttt gccgatactg ttttatagct
     1081 taaggtgtcc acttgtctta cttttttgtt taaaaacttt gacttactgt taaaacccgc
     1141 taattttcgg gattgcaaaa atacaacata tttgataaaa aaaaagaatt tgcaggaatt
     1201 tattcaggca gctcaaagca tgcttatttt tgcaaaccat ttcaacagta atgtatttca
     1261 tttaatctgc cttatggaaa aagaccgtgt ttttttatgc agggccctgg aaattgcagt
     1321 tcagggagtg aacgaggggg gagggccctt cggtgcgctg attgctaaag acggaaggat
     1381 aattgccgaa agctggaata atgttgttct ttcagcggat cccacggccc atgcagaaat
     1441 caatgccata agaaaagcct gcgaaattct cggaacccac gatcttgcag attgcatact
     1501 atactcttca tgcgaaccct gtcccatgtg cctgggcgcc gtttactggg caggaataaa
     1561 gaaggttgta tatgccttcg accggaagga ggcagaaaag gcaggcttca gcgataaatt
     1621 catctatgat gagataatgc tggaaccttc atcaaggcat gtccgcttcc ttcatattcc
     1681 cggttgctgc aacggaccgg accctttcag gaagtggaat gaaaatgaaa ataaaatccc
     1741 ctactgatat ggaaggggaa aaaaggctgt ccgcggaatt cttctcgcgg gacgttcttg
     1801 aggttgcacc tgaacttatc ggaaagtacc tggtgatccg gcggggcagt gtgccggaaa
     1861 gatatatgat tacggaaacc gaggcatacc gcgggactga agacaaggcc tgccatgcca
     1921 gccaggggga aaccccgaga aacagggtga tgtacggaag gggaggcagg atctatgtct
     1981 atctcatata tggcatgtac tggatgctga attttgtaac cggagaggaa aataatcccc
     2041 aggctgttct catcagggcc cttgaggggc tcacgggccc gggtaagctg accagggaac
     2101 tcggaataga ccgttccttt tatggtgaag accttgcagt gtctggcagg atctgggttg
     2161 aggacggcgg tatcagtccg tccttcaggc agggcccgag gataggaatt gattatgccg
     2221 gtgaattctg gaaaaacaaa ccgtggagat attatatctg ataatttttt acgttcttta
     2281 gctcaaatat tcaaaatatt tccttatgaa ttcagtattg gacattttcc gacgctggaa
     2341 caatattgcc ggatggtcag tctttgccgt ttcggcagcc gtttacctga tgactgccga
     2401 accgaccgtc agtttctggg attgcggtga attcattctt tccgcattcc ggctccaggt
     2461 cggccatccg ccgggagcac cgctctttct gatgctgggc cgtgttgcca cattctttgc
     2521 cggaggcgat gtgttgaagg tggcttttac ggtgaacagc ttttcggcta tctgcagtgc
     2581 tctgacaatc ctcttcctct tctggtcggt cacccacctc gtgaggagag ttgtaagcag
     2641 ggacggagag cttcaggcaa agcatattct tccggttata ggcagcggag ctgccggagc
     2701 cctggcttac acattttccg atactttctg gttttcggca gtggaagggg aactgtatgc
     2761 cctttcatcg ctctgtaccg cacttgtctt ctggaccatg ctcaaatggg aggaggaagc
     2821 ggacaaaccc tatgccggac gatggatcat tctcacagcc tatatcatcg gactcagcct
     2881 gggaattcac cggcttaacc tcctggtaat cccggttctc gtgtttgtgg tctattttaa
     2941 aaaatatgag atcagcggga agggaatcct gaacaccctg ctgactgcca ttctgactct
     3001 gggttttatg gtctttgtgc tcataccggg agtgccgaag gctgccggat ggtttgaact
     3061 gttttttgta aatgctctcg gacttccata taataccgga ctgttcgttt ttcttgcggc
     3121 agtgactgtt ctgctggttg ccggtatcag atattcaatc cggaagaaga atatcattct
     3181 caattatatc attacggcgc tgacggtgat aatgataggt cactcatcct atgcaatgat
     3241 catgatacgt tcatcggcca ggcctccgat gaaccagaac aatccctcag acattttcgc
     3301 cctcgggtat tatatcaata tggagcagta cggcagtgcc ccgctgcttt tcggacctta
     3361 ctacagtgca ccggccattg atgtgaaaaa cagggtgtcg ggatacaaca aggttgacgg
     3421 aaagtatgaa ccctattacc ggccggaata taaatatgac aacaggtttg agactgtctt
     3481 tcccagaatg tacagccgtg atcctgaaca tgaacaggcc tatacttact gggccgggat
     3541 aagggggaag aaatatacca tcacttccgg gtcaggcaag agaacgcttg tatgccctac
     3601 tttcggggaa aacctgaaat tcttcttcag gtatcaggca ggattcatgt atttcagata
     3661 cttcatgtgg aattttgcag gcaggcagaa cgatattcag ggcaacggaa acaagataca
     3721 cgggaactgg atatcgggat tcaggtttat tgacaatgcc aggctgggca gccaggacca
     3781 gctgccctcg gagcttctgg aaaatcccgg caggaacagg tattacatgc ttccgctgct
     3841 cctgggtata gcgggcatat tatggcagta ccgcagagac aggaagggtc tttttcttgt
     3901 tttcctgttc tttttcatga caggtcttgc aatcattgta t
//
LOCUS       DAOSPX010000310         3936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_20732_length_3936_cov_4.399124, whole genome shotgun sequence.
ACCESSION   DAOSPX010000310 DAOSPX010000000
VERSION     DAOSPX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20732_length_3936_cov_4.399124"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLZ75_12240"
     CDS             <1..272
                     /locus_tag="PLZ75_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipolytic protein G-D-S-L family"
                     /protein_id="HOS73204.1"
                     /translation="ARYLKAGLERLQSYFPELQALVKAYSRSHAGRVFMGDVKAFAYF
                     RDNYLTDFIHESGNAGTFYLHPNKKGTDALGKFWSDAIYEALTGK"
     gene            complement(278..1741)
                     /locus_tag="PLZ75_12245"
     CDS             complement(278..1741)
                     /locus_tag="PLZ75_12245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOS73205.1"
                     /translation="MNKINRRQFLEKAILYNGSLVFGASWFTSGFNYLFPQSASDYYL
                     PIQPDNPSIARYEKKCKGCRECIAVCRDVQKVYGTYKSSKTHHVCINCGACLKYCKYG
                     AMSEKYNWEDVLAAIDDPSKIVIASVSPAVPAGIGDYFGMPPGSYLAGNIISACRSIG
                     FDYVLDTNFSADLTIMEEAHELQKRIEGKVNMPQFTSCCPAWVKYVEMYYPSLINHLS
                     TTKSPIGMQGTLVKTYFAGKNDIAPENIIHVAIAPCTAKKYEITREELGINGMRSTDF
                     VITTNELAMMVKSRDVDLSAADGVFDPLMGTASGGGKLFGNTGGVMTAAIRTAHFNIT
                     GKNPSSDLIELKGVQGLSGMKSAEVNIAGTTLKVGVCYEMRNARPLLEQIMAGTCDYD
                     FIEVMACPGGCVGGAGQPALSPEILASRIEALESADSQATTRFCHENPEIKAIYKEFL
                     GEPGGPVAEQYLHTEYSDKSNLLEPALAENRLAALVV"
     gene            2491..3651
                     /locus_tag="PLZ75_12250"
     CDS             2491..3651
                     /locus_tag="PLZ75_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOS73206.1"
                     /translation="MARQIKENVFFLNATDWNRRLFDSLIPLPDGTSYNAYLVRGSEK
                     TVLIDTVDPALEDILSGYLENVEKLDFIISLHSEQDHSGSIPLVLEKYPDAMVITSPK
                     GKDLLINHLFIAEEKIITADNGEELSLGGKTLKFIHFPWVHWPETMLAYLKEDKILFT
                     CDLFGSHLATTDMYVTDECQVYEAAKRYYAEIMMPFRNFIKKNLPLLDEYDIEIIAPS
                     HGPMYDKPGFIMDAYRDWISDETKNTVVIPYISMHGSVEKMVDHLVAALEQRGVKASK
                     FDLSVTDIGKLAISLVDAAAIVVGTPTVQADPHPVVTYAVRLANMLKPKLKYASVMAS
                     YGWGGKAIERIAEMIPNIKAELIEPVLVKGYPGEDDLKSVEKLADEISSRLG"
BASE COUNT         1099 a          824 c          901 g         1112 t
ORIGIN      
        1 gtgcgagata cctcaaagcc ggactggaac ggctgcagtc atatttccct gaactgcagg
       61 cactggttaa ggcttattcc cgttcacacg cgggacgcgt attcatggga gatgtaaagg
      121 ctttcgccta tttcagggat aattatctta cagacttcat tcatgaatcg ggaaatgcag
      181 ggacatttta tctgcatccc aataaaaagg gaaccgatgc actcggaaaa ttctggtccg
      241 atgctatcta tgaagcactg acaggaaaat agtcacacta tacaacaagt gctgcaaggc
      301 ggttttccgc cagagccggc tcaagcagat ttgatttgtc agaatattct gtgtgaagat
      361 actgttcagc cacaggtcct ccgggttctc ccagaaattc cttatatatg gctttaatct
      421 ccggattttc atgacaaaac cgtgttgttg cctgagaatc ggctgactca agtgcctcaa
      481 tgcggctggc cagtatttcc gggctaagtg caggttgtcc ggcacctccc acacatccgc
      541 cgggacaggc catgacttca atgaaatcat agtcacatgt gccggccatt atctgttcca
      601 gaaggggccg ggcatttctc atttcatagc atacaccgac tttgagagta gtgccggcaa
      661 tattgacctc ggcacttttc attccgctaa gtccctgtac accctttaat tctataagat
      721 cggaagaagg attcttaccg gtgatattga aatgggcagt tcttattgcc gcagtcataa
      781 ccccaccggt attgccaaac agttttccgc ctccggatgc tgtacccatt aagggatcaa
      841 aaacgccatc ggctgccgac agatccacgt cgcgtgattt taccatcatg gcaagctcgt
      901 ttgtggtaat cacaaaatcg gtactgcgca ttccgtttat tcccagttcc tcgcgcgtga
      961 tttcatattt ctttgccgta cagggagcta tggccacatg tatgatattt tcaggagcaa
     1021 tatcattttt cccggcaaaa tacgtcttga ccagggtgcc ctgcattcct atagggcttt
     1081 ttgtggtgga cagatggttt atcaatgagg gataatacat ttcaacgtat ttaacccatg
     1141 cagggcagca actggtaaac tgtggcatgt tgaccttccc ctcaatgcgc ttctgtagtt
     1201 catgagcttc ttccattatt gtcagatcag cgctgaaatt ggtgtcaagt acatagtcaa
     1261 agccaatact gcggcatgca cttatgatat tgcccgctaa gtaggagccg ggaggcatcc
     1321 cgaaataatc tcctattcct gcaggtactg caggggaaac agacgcgatg acaatttttg
     1381 acggatcatc gatggctgcc agaacatcct cccagttata tttttcagac attgccccgt
     1441 atttacagta tttcaggcaa gccccgcagt tgatacacac gtgatgcgtt ttggaagatt
     1501 tgtatgttcc gtaaactttc tggacatccc ggcatactgc tatacattcg cggcatcctt
     1561 tgcatttttt ttcatatctg gctattgaag gattatccgg ctggataggc aggtagtaat
     1621 cagaagccga ctgagggaac aaataattaa agcctgatgt gaaccatgat gcaccgaaaa
     1681 caagggagcc gttatataga attgctttct caagaaattg tcttctgtta atcttattca
     1741 tatcataaat catttatcag tttatataca tcatttggaa attatttcgt cttcttctaa
     1801 aaaagtcagc cctgaaaatt tttcgaacaa gaatagcaaa tatttttcat ttttaaaaat
     1861 cagcccgacc agttgccact tttagaagtt cgcgggaata cctgaagttg ttcattcatg
     1921 gttttagcca ttatgtctga ctatctgatc cgcttgtaag aatcttgtgt ttccgtcacg
     1981 gaacgtttaa catccttgca gactaattgt gggacaaggc ggtttaatgc aaactggccg
     2041 gcagtattcc gggcctaatc aggatcggaa agtatcaata caaacgaaaa ggacactgta
     2101 agattctgcc taagttattc cgttaaacat ttattgctta attttggcac atcctgcagg
     2161 acacaggtgt aatcaccatc tggctcagtt caggttgatt gtaagacaga tcagttcggg
     2221 gtaattgtaa gacagatcag tttcgggtga ttgtaagaca gatcagttca gggtgattgc
     2281 aaaacggagc agttccggga aagaactatc tgaattaatc tgcagcagga aaggcagtta
     2341 aatcaggcag tgacaaactg aaatgtcact gcgtgcggtg caaactgttg ctgaaacagg
     2401 ttataccggg gctggtctga cgaaacaaat tataatacag ctgattacag cataaataat
     2461 atcaacaaat aaaacaagga gaaaattatc atggcacgac aaattaagga gaatgtgttc
     2521 tttctgaacg ccacagactg gaacaggcgt ttatttgatt cactcattcc actgcctgac
     2581 ggcaccagtt acaatgctta tcttgtcagg ggaagtgaga aaaccgtatt gattgatacc
     2641 gttgatccgg cactggaaga tattttgtcc ggctaccttg aaaatgtaga aaagcttgat
     2701 tttattattt ctcttcattc ggaacaggat cattccgggt ccatacccct tgtcctggaa
     2761 aaatatccgg atgcaatggt gattacatca ccgaagggaa aggatctgct gataaatcat
     2821 ctgttcattg cggaagagaa gatcatcacg gctgataacg gtgaggaact ttccctgggt
     2881 ggcaaaacac tgaaatttat tcattttcca tgggtacact ggcccgagac gatgctcgcc
     2941 tatttaaagg aggataaaat tctttttaca tgtgatttgt tcggatcgca cctggctacc
     3001 acggatatgt atgttacgga tgaatgccag gtgtatgaag cagccaagag atactatgca
     3061 gaaatcatga tgccgttcag gaatttcatt aaaaagaacc tgccgctgct tgatgagtat
     3121 gatattgaaa ttattgctcc gagtcacgga ccgatgtatg acaaacccgg atttataatg
     3181 gatgcctacc gtgactggat atcggatgaa acaaagaaca cggtggtgat cccttatatt
     3241 tcaatgcacg gcagtgttga aaagatggtg gatcatcttg ttgccgccct tgagcagaga
     3301 ggtgttaaag ccagtaagtt tgatctttcc gtgacggata tcggcaagct ggcaataagt
     3361 ctggtggatg ctgctgctat tgtggtaggg acaccgacgg ttcaggccga tccgcatcct
     3421 gtggtcacct atgccgtgag gctggcaaac atgctcaagc cgaagctgaa atatgcatcc
     3481 gttatggctt catacggatg gggaggaaag gcaattgaaa ggattgctga aatgattccc
     3541 aatataaagg cggaactgat tgagcctgtt ctggtgaagg gctaccccgg ggaggatgat
     3601 ttgaaatctg ttgaaaaact ggctgatgag ataagttcaa gactaggctg aaagcctgta
     3661 agagaataat ttccctgttg caagaagcga gagctaaatt atttaagagt tattgatatc
     3721 tgtaattcct gaacggatcg ctgatgcact cctgctgatc aggaagctga agattccggt
     3781 taattgttga ggagaagatc attctatcct ttatttatcc gctgcaggat tatctaaaca
     3841 aagtgaatct gcaatctgtc aggatatcat ttaatttttt atttttatct ttttgatttc
     3901 aaaacccgaa accaggaatt tgcgacagca gaattt
//
LOCUS       DAOSPX010000311         3920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_20855_length_3920_cov_4.621216, whole genome shotgun sequence.
ACCESSION   DAOSPX010000311 DAOSPX010000000
VERSION     DAOSPX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20855_length_3920_cov_4.621216"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="PLZ75_12255"
     CDS             <1..1687
                     /locus_tag="PLZ75_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73207.1"
                     /translation="PYYHLKADLELPRSSMIEVEAEVNGKVLRYLNLHKAGEEEDPSL
                     PAMTHRPPSGAGLSQDNTLYRDMSITGWVKWQPGQNYRIKLSVRMKDNIQASPKDQII
                     SAVRTLTAPEKVKTFDPAWKSYKSVVLTETAGIDRKAEPVEVLLAFYPDEAEDIKRDI
                     RVVAVDPVSYELTEVTSQVYDLQQYLVEDDLAPDENGRPTRDVPVWFPTVSARVAFLA
                     DVPARSSRIFHIYYNNPDALSKVYMSDLTVQGEGPGLQIENRHLSVVLHPKSGHLDQI
                     TLKNKSGFPLYHRKETNGAIHWNPEIYTPPRPWTHTSDWDPPQNISSYSGPVKAETAV
                     WGNLREIPEVDASVRYEFYPGKPYFISSTTMRINETMHCNALRNAEIVIKRELITHAA
                     WYDVIRDKVIIYDVTKMPDLTDLFMEADVPWITFYNQEEGVGFAGIQLEYSNSGLENP
                     ARLLNPFFYITGGPWIYWARALSLPFLSSNMQQLVPAMKGNVFSEKWAYLMYETAKGA
                     EPYAPVLEHMKALTSPLRIQLVEEVDDRVSKTVTELYVGEGRTGWEGRDTGRH"
     gene            1755..3851
                     /locus_tag="PLZ75_12260"
     CDS             1755..3851
                     /locus_tag="PLZ75_12260"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOS73208.1"
                     /translation="MNRDRYRIGISPSSEAIKIDGVLDERSWQLSDTAGNFIRVTPVD
                     TGFATAQTTVFVTYDRNNLYVGAICYDPSPGKRPVESLRRDFVFSKNDNFRIHIDTYN
                     NFTNGFVFGVSAAGAQCDGVINNGNESSFTWDIKWRSSVKNYDDRWVAEMAIPFRSLR
                     YYGGVSEWGVNFGRLDLKTNEKSAWAPVPRQFPHNSLPHAGTLVWDKAPDRAGLRMSF
                     IPYATGTIIKNTEAGEAAKWKWNAGFDTKMILSTSMNLDLTVNPDYSQVEEDRQQIDL
                     DRFELFYPERRQFFLENSDLFASLGNSQARPFFSRRIGLNVPVNFGGRLTGRIGKRWR
                     IGMMDMQTGSKGSVPSGNYAVTVLQREIFSRSSISGFLVHKQVTGTYSDTAFTGHRYN
                     SVAGMEYNYATADNRWQGKTFYHQSFYPGAGMNAAALSGKISYSSQYLKAGIDQSWIG
                     SDYIAEAGYIRRTGFYGITPSLSYLFYPPGGGRILSHGPDITINAVFDPGFRLSDRET
                     RLSYIIGWKNTSKLSLAIEEQSVKLTMDYDPTNTGSEKLEAGEQFRWKNIRAEFISDA
                     RKLFNYEMKAGMGGYYNGKRWNIDGLMNYRVQPYGSIGMTMSYNNISLPEPYNSARLI
                     LAGPTLDITFTDKIFLTTFVQYNNQIDNLNMNIRFQWRFAPVSDLFIVYTGNSYTEHF
                     GNKNRGLVVKLSYWFN"
BASE COUNT         1098 a          959 c          982 g          881 t
ORIGIN      
        1 tccgtattat catctgaaag cagatctgga gctgccccgt tcctcaatga ttgaggttga
       61 agcagaagtg aacggaaagg ttctccgtta cctgaacctt cacaaggctg gagaggagga
      121 agatccttcc ctcccggcaa tgacccatcg tccgccttcg ggtgcaggcc tttcccagga
      181 caacaccctg tacagggaca tgagcatcac gggatgggtc aaatggcagc ccggacagaa
      241 ttaccggata aaactcagtg tgaggatgaa ggataatatc caggcttcac ctaaagatca
      301 gatcatttca gcagtgagga cacttactgc tcctgaaaag gtaaaaacat tcgaccctgc
      361 ctggaaaagc tataaatcgg ttgttctgac cgaaaccgcg ggaatagaca gaaaggctga
      421 acccgttgag gttctccttg cattctatcc cgacgaggca gaggatatca agagggatat
      481 aagggtggtt gcagttgatc ctgtcagcta tgaactgaca gaagtcacgt cacaggttta
      541 tgatcttcag cagtatctgg tggaggatga ccttgccccc gatgaaaacg gaagacccac
      601 cagggatgtt cctgtctggt tcccgacagt gtcggcaaga gttgccttcc tggcagatgt
      661 gccggcccgc tccagccgta tcttccatat ttactacaac aacccggatg cactgtcgaa
      721 ggtgtacatg tcggatctga ctgttcaggg cgaaggcccc ggactccaga tcgagaaccg
      781 gcacctgtcg gttgtccttc atcccaaatc agggcacctc gaccagataa cactcaagaa
      841 caaatccggt ttcccgcttt atcaccgcaa ggaaacaaac ggtgccatcc actggaatcc
      901 tgaaatctat actccgccca gaccctggac ccatacatcc gactgggatc cgccacagaa
      961 cataagttca tactccggtc cggtgaaggc agaaacagct gtatggggca atctccggga
     1021 aatacccgaa gtggacgctt ccgtcagata tgagttttat cccggcaaac cctatttcat
     1081 cagttcaaca acgatgagga taaatgaaac catgcactgc aatgcactcc gcaatgccga
     1141 gatagtgata aagagggaac tgataaccca tgccgcatgg tatgacgtta tcagggataa
     1201 ggtcatcata tatgacgtaa cgaagatgcc ggatctgacc gacctcttca tggaagcaga
     1261 tgttccgtgg atcactttct acaaccagga ggaaggagtg ggttttgcag gcattcagct
     1321 tgagtattca aattccggac tcgagaatcc tgccaggctt ctcaatccct tcttctatat
     1381 aacaggaggc ccgtggatat actgggcaag ggcattatcc ctgcccttcc tttcctcgaa
     1441 catgcagcaa ttggtgccgg caatgaaagg aaacgtattt tcagaaaaat gggcttacct
     1501 gatgtatgaa acggcaaagg gtgcggaacc ctatgctcct gttcttgaac atatgaaagc
     1561 actgaccagt cccctgcgca ttcagctggt ggaagaagtg gatgacaggg tgtcaaagac
     1621 agttaccgaa ctctatgtag gcgaaggcag aacaggttgg gaagggcgtg ataccggaag
     1681 gcactagaaa atttttgctt ttttgcctct tcttcctttc cggaggaata gttattcatt
     1741 gccaggaaaa ggcagtgaac cgtgacaggt accgtatagg tatatcacca agcagcgagg
     1801 cgataaagat tgacggagtg ctggatgaga gatcttggca gctttcggat acagccggga
     1861 atttcatccg tgttacaccg gtggataccg gctttgcaac agctcagacc accgtgttcg
     1921 tcacctacga caggaataac ctgtatgtcg gggccatatg ctatgatccc tctcccggca
     1981 aaaggccggt ggagtccctt cgcagggact tcgttttcag taaaaatgac aacttcagaa
     2041 tacacataga tacctataat aatttcacca acggttttgt tttcggtgtc tcagctgccg
     2101 gagcccagtg cgacggtgtg ataaacaacg gaaacgagtc aagcttcacc tgggatatca
     2161 aatggagatc ttcagtgaag aactacgatg accgctgggt tgccgagatg gcaattccgt
     2221 tcaggagcct tcgctattac gggggagtaa gcgaatgggg agtcaatttc ggccgtcttg
     2281 atcttaaaac gaatgaaaaa tcagcatggg cccctgtccc ccgccagttt ccgcataatt
     2341 cccttccgca tgcaggaacg ctggtatggg acaaggcccc tgacagggcc ggactgcgta
     2401 tgtcattcat cccctatgcc acgggaacga tcataaaaaa cacagaagcg ggagaggcag
     2461 caaaatggaa atggaacgcg ggctttgaca caaaaatgat attatccact tccatgaacc
     2521 tcgatctgac tgttaatccc gattattcac aggtagagga ggacaggcag cagattgacc
     2581 tcgaccgctt tgaattgttt tatccggaaa ggcgtcagtt tttccttgag aacagcgacc
     2641 tcttcgccag ccttggcaac agccaggcac ggcctttctt ttcaagacgc ataggactaa
     2701 atgttccggt gaatttcgga ggaaggctta ccggaaggat agggaaaaga tggaggatag
     2761 ggatgatgga tatgcagacg ggctcaaagg gttcagtccc ttcaggaaat tatgcggtga
     2821 ctgtccttca gcgggagatc ttcagccgtt ccagcatctc cggcttcctt gtccacaaac
     2881 aggtcaccgg aacttacagt gacacggcct tcaccggtca ccgttacaac agtgtggcag
     2941 gtatggaata caattacgca acagccgaca accgctggca gggaaaaaca ttctaccatc
     3001 agtcattcta ccccggagcg ggaatgaacg cagccgccct ttcgggaaaa ataagctact
     3061 cctcacagta tctgaaagca ggtatagacc agtcatggat aggttcagac tacatcgccg
     3121 aggcgggtta cataaggaga acaggcttct atgggatcac gccctccctg agctatcttt
     3181 tctatccccc gggaggcgga aggatactca gtcacggccc cgacatcacc atcaatgcgg
     3241 tttttgatcc gggattcagg ctcagcgacc gggaaacaag gctttcatat attatcggat
     3301 ggaagaatac cagcaaacta agcctggcca ttgaggaaca atcagttaaa ctgaccatgg
     3361 attatgatcc gaccaataca ggaagcgaaa aacttgaagc aggtgaacag ttccggtgga
     3421 agaatataag ggctgaattt atttccgacg cccggaaatt attcaactat gaaatgaaag
     3481 caggcatggg aggctattac aacgggaaac gctggaatat tgacgggctg atgaactacc
     3541 gcgtacaacc atacgggagc ataggaatga caatgagcta taacaacatt tcattgcctg
     3601 aaccctataa cagtgcgagg ctcatccttg caggacccac actcgatata accttcacgg
     3661 ataaaatatt cctgaccaca ttcgttcaat acaacaacca gatagataat ctgaatatga
     3721 atatccgctt ccagtggcgc tttgcaccgg tatcggacct cttcatcgtg tacacgggga
     3781 attcatacac ggaacatttc gggaacaaga accgcggact ggtggttaaa ctatcctact
     3841 ggtttaactg attccgaaaa ccgaagaaag cgggctggtg gttaaactgt catactggtt
     3901 taacagactc cgtaaacagg
//
LOCUS       DAOSPX010000312         3916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_20874_length_3916_cov_5.309764, whole genome shotgun sequence.
ACCESSION   DAOSPX010000312 DAOSPX010000000
VERSION     DAOSPX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20874_length_3916_cov_5.309764"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PLZ75_12265"
     CDS             complement(<1..1041)
                     /locus_tag="PLZ75_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS73209.1"
                     /translation="MTSRDRLLLSLSHREPDRIPVDLGSSTVTGISGIAWNNLRKYLN
                     ISGRTRIFDVVQQLAMVEMDIIDLFGVDVLDANRVSIENTGWYEVSLADGSPAEYPSW
                     FRPQKNEDGSWHTYDRDGTVIAKMAAGATFFDQMHYPYEDAYPENFNGLKDALKKISW
                     IVHSHASNLDVSELRSRLTGLREETGKALVMSGGVKLLELGFFIRRMDNFLMDLIVDG
                     DKVSELLDILVEMHLASLEKKCSALGDIVDVIRFGDDLGMTTAPFMDLETFRKLFKPR
                     YKILCDYVKKNSNMKIFLHSCGSIRQYIPDLIDAGFDIINPVQTNCHDMDPVELKKEY
                     GRDIVFWGGGVDT"
     gene            complement(1118..2239)
                     /locus_tag="PLZ75_12270"
     CDS             complement(1118..2239)
                     /locus_tag="PLZ75_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOS73210.1"
                     /translation="MENKTKTASKRLYSLDALRGFDMFWIMGGEGIFISLAAITGWPV
                     FNWCANQLEHVPWHGFVFYDMIFPLFLFIAGISFPFSYAKRTANNESRKSIYRHVFYR
                     GLILVLLGILYNNGLRFNFPELRYGSVLGRIGLAWMFAALIFMNTRLTARIIWCSGIL
                     IAYWLLLLLFPAHDLGSNDPFSQEGNLASYIDRLIMPGKLYRGNHDPEGIFSTLPAIS
                     TALLGMFTGEFLLSEKYRDRPYRKVLYMFIAAVVLMIIGKLWDLVFPINKNLWSSSFV
                     CYVGGLSLLLFVIFYLVIDVWNIRKWAFFFVVIGVNPITIYLAGRIINFQAATRFFFG
                     GFAALVPEAWVPFVNAVGLTTIGWLFLYFLYRKKIFLRV"
     gene            2593..>3916
                     /locus_tag="PLZ75_12275"
     CDS             2593..>3916
                     /locus_tag="PLZ75_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOS73211.1"
                     /translation="MDVSGPGSVLVISFVGYDVAEVPVGNQTTFNIRLTESMLGLEEV
                     VVVGYGTQKKLNLTAAVDQVTNETLENRSVPNLTQGLKGVLPNLNINLLDGKPIQAPS
                     YNIRGATSIGQGGSALILIDGVEGDPSLLNPNDIASVSVLKDAASAAIYGARGAFGVV
                     LITTKNPEKGKTSITYSSNYSIKKPTVPDFVTDGYTFLKLFAESFINGDGSFPQNVNK
                     ETRFSQAYLDEFKRRAESGQPYNEVEIDPVTGEYVYYGNTDWYALLYKDFTTAADNNI
                     TISGSGEKAAYLVSGRIMDQPGIFRYSSDDYSMINLRAKGSIQVLPWLMLENNTDFST
                     MKYYNPFSVGEGAVDIWNIIAVDTHPHSPMFNPDGTLTTGAVYSVGDFWYGKNGITMK
                     KAVFKNTSGFTASFFDKKFRLRGDFTVRKDDNENVTKRVPVPYSSKPGV"
BASE COUNT         1096 a          888 c          906 g         1026 t
ORIGIN      
        1 agtatcaacc cctcctcccc agaacacaat atccctgccg tattccttct tcagttcaac
       61 aggatccatg tcatggcagt tggtctgaac ggggttgatg atgtcaaatc ccgcatctat
      121 cagatccgga atatattgcc ttatgctgcc gcatgaatgc aggaaaatct tcatgttgct
      181 gtttttcttt acgtaatcac agaggatttt gtatctgggt ttaaacagtt ttctgaatgt
      241 ttcaaggtcc atgaagggag ctgtcgtcat ccccagatcg tcgccaaagc ggatcacgtc
      301 cacaatatcg cccagggcac tgcacttttt ctccagcgac gcgagatgca tctcaacaag
      361 aatatcgaga agttccgaca ccttgtcccc gtcaacaatg agatccatca ggaaattatc
      421 cattcttctg atgaaaaagc caagttcaag gagctttacc cctccggaca ttacaagagc
      481 cttgcctgtt tcttccctga ggccggtcag tctgcttctg agctcgctta cgtccaggtt
      541 cgaagcatgg gaatgaacga tccatgaaat cttcttcagg gcatccttca gtccgttaaa
      601 gttctccgga taagcatctt catagggata gtgcatctga tcgaagaaag tggccccggc
      661 tgccatcttt gcgataactg ttccgtcacg gtcatatgtg tgccaggaac cgtcttcatt
      721 tttctgcgga cggaaccatg acggatattc agccggactg ccgtctgcga gagatacttc
      781 gtaccatccg gtattctcaa tggatacacg gtttgcatca aggacatcaa cgccgaaaag
      841 atcgattata tccatctcta ccatggccag ctgctgaacc acatcaaaaa tccttgtgcg
      901 gccactgatg ttcagatact tcctgagatt gttccaggca attcctgata tccccgtaac
      961 cgttgaactg ccaagatcaa ccggtatcct gtcgggctcc cggtgactga gagataataa
     1021 taatctgtct cttgatgtca tgggctgatt gtaatatagc ttcttttccc gccggtgcag
     1081 agtgttctgt tccggatatt atatttcatt ctgattatca gactctaagg aaaatctttt
     1141 tcctgtaaag gaaatacagg aacagccatc ctatggttgt cagtcctaca gcatttacaa
     1201 acggtaccca ggcttcaggg accagggctg caaatccgcc gaaaaagaat ctggttgctg
     1261 cctgaaaatt aatgattctt ccggccagat aaatagtaat cggattgact cctatgacta
     1321 caaagaaaaa ggcccatttc ctgatattcc agacatcaat gacaagatag aagatcacaa
     1381 agagaagaag gctcagtccc cccacatagc aaacgaaaga gctggaccat aaatttttat
     1441 tgatggggaa gaccaggtcc cacagttttc cgattatcat caaaactact gcagctataa
     1501 acatatacag aactttccgg tacggccggt ctctgtattt ttccgacaag agaaactccc
     1561 cggtgaacat gccgagcaat gctgtcgaaa ttgcgggcag ggtgctgaat attccctctg
     1621 gatcatgatt gccgcgatat agtttaccgg gcattataag acggtctata tagcttgcaa
     1681 ggttgccttc ctgtgaaaag ggatcgtttg atccgaggtc atgtgccgga aagaggagca
     1741 gaagcagcca gtacgcgatc agtattccgc tgcaccatat gatacgggcc gtcagcctgg
     1801 tattcataaa gataagtgcc gcaaacatcc atgccagccc tattcttccg agaacacttc
     1861 cgtaacggag ttccgggaaa ttgaatctga gtccgttatt atacagaatt cccagaagta
     1921 ccaggatcag gcccctgtag aagacatgcc tgtagattga ttttctgctt tcattgtttg
     1981 ctgtcctctt tgcataggaa aagggaaagg atattccggc aataaagagg aagaggggaa
     2041 agatcatgtc gtaaaacaca aagccgtgcc agggcacatg ttcgagctgg tttgcgcacc
     2101 agttaaaaac gggccagccg gttatggcag ccagggaaat aaaaatacct tccccgccca
     2161 taatccagaa catgtcgaat ccgcggagag cgtcaagtga ataaagcctt ttcgaagcag
     2221 tttttgtttt gttttccata ttatcaggtt gtgaaatata agtgttgaaa ttaatttaag
     2281 aaatgaagaa cgtaaatata atacttgttg aacaagctca ggccatatgt tttattttag
     2341 tttggatgat gcggcatttc atatattttt taataccttt caaacgtgaa aatttaatat
     2401 taatcctaaa cttaagccta tgaaaaatat tttgttaatg ctgttactgt taatgtcatc
     2461 agtattgtac ggacaacagg taacagtatc aggaactgta acggattccc agggtgtccc
     2521 gctcccggga gcgacggttc agatcagggg aacaaatgtg gggccctcac tgatgcggga
     2581 gggaaatttt ccatcgatgt ttctggtccg ggatccgtac tggttatatc ctttgtcgga
     2641 tatgacgtgg cggaagtgcc tgtgggcaat cagaccactt ttaatatcag gcttaccgaa
     2701 agcatgctcg gtcttgaaga agttgtggta gtcggttatg gcacacagaa aaaactgaat
     2761 cttaccgccg ctgttgacca ggtgaccaat gaaactcttg aaaacaggtc tgttccgaac
     2821 cttacccagg ggctcaaggg agttttaccc aacctgaaca tcaatctgct ggacggtaaa
     2881 cccattcagg cacccagtta caacatcagg ggtgccacat cgataggcca ggggggaagt
     2941 gccctcattc ttattgacgg ggtggagggc gatcccagcc tgcttaatcc caacgatata
     3001 gcaagcgttt cagtgctcaa ggacgctgct tcggcagcaa tctacggcgc caggggagct
     3061 tttggcgtcg tgcttattac aaccaagaat ccggagaaag ggaaaacgag tataacatat
     3121 tcaagtaatt attcgattaa aaaaccaact gtacctgatt tcgtgaccga cggttatacc
     3181 tttctgaaat tgtttgcaga atcattcatc aatggtgatg gttcatttcc tcagaatgtc
     3241 aataaggaaa ccaggttttc acaggcttat ctggatgaat ttaaaagaag agcggaatcg
     3301 ggccagcctt ataatgaagt ggaaatcgat cctgttacgg gtgagtatgt gtattacggg
     3361 aatacagact ggtatgcatt gctttacaag gatttcacca ccgcggccga taataacata
     3421 acaatatcgg gaagcgggga aaaagctgcg tatctggttt ccgggagaat aatggatcag
     3481 cccggaatat tccgttacag ttcggatgac tattccatga taaacctgag ggcgaaagga
     3541 tccatacagg tgctgccctg gctgatgctt gaaaacaaca ccgatttctc cacaatgaaa
     3601 tattacaatc cattttcggt tggcgaaggt gctgtggata tctggaatat cattgcagtt
     3661 gatacccacc cgcattcccc gatgttcaac cccgacggaa ccctgactac cggtgccgtg
     3721 tacagcgtgg gtgatttctg gtacgggaaa aacggtataa ccatgaaaaa agctgttttc
     3781 aagaatacca gcggattcac ggcaagcttt ttcgataaga agttccgcct gaggggagat
     3841 tttaccgtca ggaaggacga taatgaaaat gtgacaaagc gggtacctgt tccgtacagc
     3901 agcaaacccg gagtat
//
LOCUS       DAOSPX010000313         3913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_20897_length_3913_cov_4.955417, whole genome shotgun sequence.
ACCESSION   DAOSPX010000313 DAOSPX010000000
VERSION     DAOSPX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20897_length_3913_cov_4.955417"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3306)
                     /locus_tag="PLZ75_12280"
     CDS             complement(<1..3306)
                     /locus_tag="PLZ75_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOS73212.1"
                     /translation="MGKRKRSYILIILSMIFGLAAAIAQMICQGAPENSIITRRFNRV
                     LHEKEKIMEDCLNDLRTIMIKGEPHGSVSENRIFSLASQNEITILQYIDRRLVYWSDN
                     SFDVPVLLQDESLFSRPLIFMQNGWFLPSHIAEGNERIIGLLRVRTDYSFENDIVKSG
                     FARDFGLPPDVGFSVEKSENGNDVFNRKGEFLFSLVLPAAGVTTSHILIPLVLWCVFF
                     LLILAFVLGAVKSLAASGKSSSGIIVSLLFFSVLYFTVLFARQPSVIFRTTLFSPYIF
                     SLNAFIPSLGHFMIFSILSALAGYVFYNYADFSKIYGERKPGSRLLPDLLLVSGTLLI
                     AMFHMVFRRLILNSNINFETYKVLDLSAYSAVGFISVLLLLVLPVSFICKAFQTGGRP
                     GGFRTIPVLAVSAGLLILATYSDRISMAALLFLWAGIIAAVWVHQFRQPGRLSIPLFL
                     SLAAGIYSLLVISTFSEEKTNEILKVHALSLSTENDPEAEHLLLDLWPLLQKDAVLAD
                     MMDVEFFERRDFNIISAYLNNNYFNGYWGNFNVSIYLCRQGQLIRIGPGNHDFEDCYA
                     FFEERIDKYGHPLTGTQFYFIDNQGARPYYIGRLFFETGSRITNGLFIELYGDINVFQ
                     PGYSELLLDKRFHGYSGLKNYSFAKYLNGEIILSHGDFPYNKADEEYVDEAEDYRIFD
                     NEGFRHILYRNGNATVMISRPLLNAGKIIISFAYLFAFTFIVVNSILLFFASPSRTGI
                     RSLNFRQKLQISFTGILLFSFILVGIAVASFTIKEFHSNHYNNIKEKLNSIYTELEAE
                     ISMERHLTPNWRNSNNASLNELLINLSNIFNTDVNLYDLNGYLMATSRPELFFRDLTS
                     HRMNMKAFMNVSDLTKSEYIQTESVGNMKYISAYVPFYNVEKHILAYLNLPYFRMQSL
                     LAREISDMIVAIINFTLLIIVITMVFVVFISGRLTSPLSMLGEGLASVRLGRKSEHLT
                     YRGRDEIGDLVRQYNIMVDELEESAHKLANSEREYAWREMAKQIAHEIKNPLTPMKLN
                     VQQLQKSWNDRIHDFGEKLEIFARNQIEYINNLSNIATAFSSFAKMPGANPVEVDLLD
                     QIGVVLADGDAVVL"
     gene            complement(3319..3792)
                     /locus_tag="PLZ75_12285"
     CDS             complement(3319..3792)
                     /locus_tag="PLZ75_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOS73213.1"
                     /translation="MKIVFLQTGKTSEKHIAEGVENYFLRLEKNPGLEIITVPDIRNT
                     ANLTKEEIRKKEGGKILGKIYGTDYVVLLDERGREFRTVEFASWLENRLMQAERRLLF
                     VSGGAWGFSEQVYARADMKISLSKMTFPHQLVRLIFLEQLYRALSIIKGSPYHHE"
BASE COUNT         1095 a          918 c          867 g         1033 t
ORIGIN      
        1 gagtactacg gcatcgccat ccgcaagaac aacgccgatc tgatcaagaa gatccacttc
       61 aacgggattg gccccgggca tctttgcgaa tgaagaaaac gcagtggcta tatttgaaag
      121 attgttgatg tattctatct ggttcctggc aaaaatttcc agtttttccc cgaaatcatg
      181 aatcctgtca ttccacgatt tttgcaactg ctgaacatta agtttcatgg gtgtaagggg
      241 attcttgatt tcatgggcga tctgcttcgc catttccctc catgcatatt ccctttccga
      301 attggcaagc ttatgggcac tctcctccag ttcgtcaacc ataatgttgt actgcctgac
      361 caggtcgcct atctcatccc ttcccctgta ggtaagatgc tcactctttc tgcccagcct
      421 cacggaagca agtccctcac cgagcattga aaggggggag gtgagcctgc cgctgataaa
      481 aacaacaaag accatggtga tgactatgat aagaagcgtg aaattgatta tggccactat
      541 catgtcggaa atctcccggg caagcaggct ctgcatcctg aaatagggca gattcagata
      601 ggccaggata tgcttttcaa cgttgtagaa aggcacatag gccgatatgt atttcatgtt
      661 ccccacactt tccgtctgga tatattcact cttggtcaga tccgaaacat tcataaaggc
      721 cttcatgttc atcctgtggc tggtcagatc cctgaaaaag agctcgggac gggatgtggc
      781 catgagatat ccgttgaggt cataaaggtt tacatctgta ttgaagatat tggacaggtt
      841 gatgagaagc tcattcagcg aggcattatt gctgttgcgc cagtttggag tcagatgcct
      901 ctccattgat atttcggctt caagttctgt atagatggaa ttaagttttt cctttatgtt
      961 gttgtagtga tttgaatgaa actcctttat ggtgaaggag gctactgcaa taccgaccag
     1021 gatgaaggaa aataacaata taccggtgaa ggatatctga agcttctgtc tgaaattaag
     1081 actcctgatt ccggtccgcg aaggagatgc gaaaaaaagc agtatggaat tgacaactat
     1141 gaaggtgaag gcaaaaagat aggcaaagga tatgattatt ttccctgcat tcagaagggg
     1201 cctgcttatc atcaccgttg cattcccgtt gcggtaaagg atgtgtctga aaccctcatt
     1261 gtcaaatatg cggtaatctt cagcctcgtc aacatattcc tcatcagcct tgttgtatgg
     1321 gaaatcccca tggctgagaa taatttcccc gttcagatac ttggcaaagg aataattctt
     1381 cagacccgaa tatccgtgga accttttatc cagcagcagt tccgaataac ccggctgaaa
     1441 cacattgatg tcgccataca gctcaataaa cagcccgttg gtaatccggg acccggtttc
     1501 gaaaaagagt cttccgatat aatacggccg ggctccctgg ttgtcaatga aatagaactg
     1561 ggtgcccgta aggggatgtc cgtatttgtc aatcctttcc tcaaagaaag cataacaatc
     1621 ctcaaagtca tgatttcccg gacctatccg tatcagctgt ccctgcctgc acaggtatat
     1681 gctgacattg aaattgcccc agtatccgtt gaagtagttg ttgttcagat aggcggatat
     1741 tatattgaaa tctcttcttt caaagaattc aacatccatc atatcagcca ggaccgcatc
     1801 tttctgcagc agcggccaca gatccaggag gaggtgttcg gcttccggat cattttcagt
     1861 tgaaagggac agggcatgga ccttgaggat ttcattggtc ttttcttctg aaaatgtgct
     1921 gatgaccaga agcgaataaa tgccggctgc cagggagaga aacaggggaa tgctgagcct
     1981 tcccggctgt ctgaactgat gtacccatac tgcagctata atgccggccc acaggaaaag
     2041 gagcgccgcc atggatatcc tgtcgctgta tgtggcaagg attaaaagcc cggctgacac
     2101 agcaaggacc ggaattgtcc tgaagcctcc gggtctgcct ccggtctgaa atgccttgca
     2161 gataaaagaa accggcaaaa ccagcaggag caggaccgaa ataaagccca cggcactgta
     2221 tgcgcttaaa tccagaacct tataggtttc aaaattaata tttgaattca gtatcagcct
     2281 gcggaaaacc atatggaaca tggctatcag caatgttccc gaaacaagaa gcagatccgg
     2341 cagcaagcgg ctgccaggct tcctttcccc atatattttg ctgaaatccg catagttgta
     2401 aaaaacatag ccggccaggg ccgacagtat gctgaaaatc atgaaatggc ccagggaggg
     2461 aatgaaggcg ttaagtgaga atatataggg agaaaacaat gttgttctga aaatcactga
     2521 aggctgtctg gcaaaaagaa ctgtgaagta caggactgaa aaaaacagca gtgaaactat
     2581 tatgccgcta ctgctttttc ctgaagcagc cagtgatttc accgctccga gcacaaaggc
     2641 aagaatgagc agaaagaaca cacaccacag aaccaggggt atcagaatat gagaggtggt
     2701 tacgcctgcc gccggcaata caagcgaaaa cagaaactca ccctttctgt tgaacacatc
     2761 attgccgttt tcagactttt ccacactgaa gcccacatcc ggggggagcc cgaaatccct
     2821 ggcaaagccg ctttttacaa tatcattttc aaaactgtaa tcggtcctca ccctcagcag
     2881 cccgataatc ctttcattcc cctcagcaat atgagaaggc agaaaccagc cgttctgcat
     2941 aaagatcagg ggccgggaga ataatgattc atcctggaga agcaccggaa catcaaaact
     3001 gttgtcagac cagtaaacca gtctcctgtc aatatactgg agtatcgtta tttcattctg
     3061 ggatgcaagg ctgaatatcc tgttttcgga aacggaaccg tgaggctcgc cttttatcat
     3121 aatagtcctg agatcattaa ggcagtcctc cattatcttc tccttctcat gaaggaccct
     3181 gttgaatctt ctggttatta tgctattctc aggcgctccc tgacagatca tctgggcaat
     3241 ggcggcagcc agcccgaaaa tcatgctcag aatgatcagg atgtagctcc tcttcctttt
     3301 tcccataata aattactgtt actcatgatg gtatggagat cccttgatga ttgacaaagc
     3361 cctgtagagc tgttcaagga atatcagcct cacgagctga tgcggaaatg tcatttttga
     3421 caatgagatc ttcatatcag cccttgcata tacctgttcc gaaaagcccc atgccccgcc
     3481 ggaaacaaac aataatctcc tctctgcctg catcagtctg ttttcaagcc aggaagcaaa
     3541 ttcaacggtc cggaattccc tgccacgctc atccaggaga accacataat ccgtaccata
     3601 tatctttccg agtatttttc cgccctcctt tttccggatt tcctcctttg tcagattagc
     3661 ggtatttctt atatccggta cggtaattat ttcaagtccg ggattctttt caagcctcag
     3721 gaaatagttt tcaactccct cggcaatatg cttttcactg gtttttccag tctgtaggaa
     3781 gacaattttc ataaacggcc cgacttttgt atgatgatat atgcgatgcc aagctgcaaa
     3841 cttataatta aaaacggcct gatgcaatta taattatatt gataaggcag aaccgtttat
     3901 atttctggaa aat
//
LOCUS       DAOSPX010000314         3879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_21117_length_3879_cov_3.593619, whole genome shotgun sequence.
ACCESSION   DAOSPX010000314 DAOSPX010000000
VERSION     DAOSPX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21117_length_3879_cov_3.593619"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLZ75_12290"
     CDS             <1..238
                     /locus_tag="PLZ75_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73214.1"
                     /translation="GEYVNEAEQTALSTLTAIMGRTAAYTGRAVTWDDILKSDMFLGP
                     SKLEFGKVDMVFEAPVPGTPVITTVKPAYKGQII"
     gene            246..1559
                     /locus_tag="PLZ75_12295"
     CDS             246..1559
                     /locus_tag="PLZ75_12295"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS73215.1"
                     /translation="MSGKTSRRKFIRRSAAIAAGAFVIPEIIPSTVLGMGGVKPPSDR
                     VVIGAIGTGSQGMANMRDFLRLRDEVRFTALCDVDSVRLDNAKRVVDTANKSNDCRTY
                     VDFREFLEKETLDAVSIALPDHWHGIIYTAAVNKKLHVYGEKPICRTISDGQAIVSAV
                     RSNNIIWQTGSWQRSQANFHRGAELVANGRAGKITRIVVGLPDGGRSIGTPPVMDPPP
                     ELDWDMWLGPAPKVPYRGVSHWDWRWILDYSGGQLTDWAGHHIDIANWGAGLEHTGPE
                     EISGSGVYPPEGIYDAPVEYDFLCRYANGIEMRVANASRLEHGMGTTWYGDLGWIHTD
                     RGNVLTASDPEILKEVIGEDEIHLYKSENHWQNFIDSLRSGRQAVSPVDSAYRAISVA
                     LLGEIAMTTGETIKWDPDKEEIIGNPRASRLLSRPYRKPWSLPEA"
     gene            1603..2724
                     /locus_tag="PLZ75_12300"
     CDS             1603..2724
                     /locus_tag="PLZ75_12300"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOS73216.1"
                     /translation="MKRQYKFSLPAVLLLTAVLMLAVTSCRDKAAYRALIISGQAEYD
                     RETASQAVKQILDASGLFSTDILIAPEKGEDLSGLSKEFSKYNLIAIAYRGDIWPENA
                     YGAFTAFLEGGGGLLVYYPKTRFGETERDEPVSSAISRSHTFEVKTVQTDHPVTSGLP
                     VRWLQPEDIIVPSPVLPGSGIKVLATAFSDTAYGGSGRAEPVLLAGSEGGGRIFATMI
                     GLPGEEQEQAVHSTGFIATLQRGAEWAASGNVTQAVPSDFPTAAAAVTRTGFRETSTA
                     EAFRMMAAYDIGKSTRYYTHIREEIRKAGGDQKILSGLEREMVKILNSSAATAEAKKL
                     MLRELGWMGSDYCVNAVKRLIEVPELKEEAESALERLGK"
     gene            2705..3676
                     /locus_tag="PLZ75_12305"
     CDS             2705..3676
                     /locus_tag="PLZ75_12305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS73217.1"
                     /translation="MRGSGNNRLLMFSLVIPVFNEEKLIDELVRRTIPAIESFTDDYE
                     VIFVDDGSTDRSAGMILGYREKNKKIKLLSLSRNFGHQAAYTAGLEYSSGDIAGMMDG
                     DLQDPPEILRDMYNKMKEEDLDVVSGIRSGRKGSRGRNLTSRTFHALFRHAGDLANIE
                     NSGNFSIMKRIVVDAVLSMREKVRCLPGLRAFAGFRQGYVEYVRDGRYGGRPKMTFAN
                     LFTLAGDAIFSFSKLPVRICLILGMTGTVVFLGAGIYLLIARAAGAAVPGWSPALLSL
                     YFLGSVQLVFLGILGEYIYRNYKESQNRPIYFARKFYDEYDGNEEEG"
     gene            3660..>3879
                     /locus_tag="PLZ75_12310"
     CDS             3660..>3879
                     /locus_tag="PLZ75_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73218.1"
                     /translation="MKRKDERPIWKRIFFIASALYLILMLFLSRDAGISCDDTLHYDH
                     SVDVYNYFATHGSDRSALETPVTHLKYYG"
BASE COUNT         1033 a          865 c         1088 g          893 t
ORIGIN      
        1 cggagagtat gtcaatgaag cggaacagac cgcactgtcg acactgactg ccatcatggg
       61 cagaacagct gcctatacgg gccgggctgt cacatgggac gatatactga agtcggatat
      121 gttcctgggt ccttcaaagc ttgaattcgg gaaggtagac atggtgtttg aagccccggt
      181 tcccggaact cccgttatta caacagtaaa accggcctat aagggccaga taatataatc
      241 gctatatgtc aggtaaaaca tccagaagaa aatttataag gcgttcggct gcaatagcgg
      301 ccggtgcttt cgtgatcccc gaaataattc cttccaccgt actgggtatg ggaggagtaa
      361 agcccccgtc ggaccgtgtg gtaatcggcg caataggtac gggatcacag ggtatggcca
      421 atatgaggga tttcctgagg ctcagggatg aagtccgctt tacagccctt tgtgatgttg
      481 attcagtcag actggacaat gccaaaaggg tggtggatac tgcaaacaaa agcaatgatt
      541 gccgcaccta tgtggatttc agggaattcc ttgaaaagga aacactcgat gcagtatcaa
      601 tcgctctgcc ggaccactgg cacgggatta tctatactgc ggcagtgaac aaaaaactgc
      661 atgtgtatgg tgagaagccg atatgccgga ccatcagtga cggtcaggca atagtctctg
      721 ccgtccggag caataacata atctggcaga caggcagctg gcagaggtca caggcaaact
      781 ttcacagggg agccgaactg gttgccaacg ggagggccgg gaaaattaca cgtattgtgg
      841 ttggactgcc tgacggggga agaagcattg gtacaccgcc ggtgatggat cctcctccgg
      901 aactggactg ggacatgtgg ctcggacctg caccgaaagt gccataccgg ggggtatctc
      961 actgggactg gcggtggatt cttgattatt cgggaggcca gctcactgac tgggccggac
     1021 atcatatcga cattgcaaac tggggtgccg gactggaaca tacgggtccc gaggaaattt
     1081 ctggctccgg cgtatatcct cccgaaggca tttatgatgc accggtggaa tacgatttcc
     1141 tgtgcagata tgccaatggt atcgaaatga gggtagccaa tgcctcaagg cttgaacacg
     1201 gtatgggaac aacatggtat ggtgaccttg gatggataca caccgacagg ggaaatgttc
     1261 ttacagcttc agatcctgaa atattaaagg aagtgattgg tgaggacgag atacatctgt
     1321 ataagagcga aaaccactgg cagaatttta ttgattccct gcgatcgggc agacaggctg
     1381 tttcaccggt tgattccgcc tatagggcga tatccgttgc attgctgggg gaaattgcca
     1441 tgacaacggg agagacaata aaatgggatc cggataagga ggaaataatc ggaaatcccc
     1501 gggcatcacg gctgctcagc cggccatacc gtaaaccatg gtcattgccg gaagcataaa
     1561 ccccttgaca gtatgaatcc gcaacagcat aaattcaaaa agatgaaaag acaatataaa
     1621 ttttcccttc cggctgtact gcttcttact gcagtattaa tgcttgccgt cacttcctgc
     1681 agggataaag ccgcttacag ggccctgatt ataagcggac aggcggaata tgacagggaa
     1741 acggcctcac aggccgttaa acagatactg gatgcctccg gtctgttttc gacggatatt
     1801 ttaattgccc cggagaaagg agaagatctt tcagggctca gcaaggaatt ttcaaaatat
     1861 aatctgatag ccatagccta ccgcggggat atatggccgg aaaatgcata cggagccttt
     1921 accgctttcc tggagggagg aggaggactg ctggtttatt atccgaaaac ccgctttgga
     1981 gaaacagaaa gggatgaacc cgtttcctca gccatttccc gcagtcatac atttgaggtg
     2041 aagactgttc agactgatca tccggttacc tcagggttac cggtcagatg gctccagcct
     2101 gaggatatta ttgtaccgtc accggtgctg ccgggcagcg gaataaaggt cctggccacg
     2161 gcattttcgg atactgccta cggcggttca ggcagggcag aaccggtgct tctggcgggg
     2221 agtgaaggcg gcggaagaat atttgccacg atgatcggac tgcctggaga ggagcaggaa
     2281 caggctgtgc acagtacggg ttttattgca acactgcagc gtggcgccga atgggctgca
     2341 tcaggaaatg tgacgcaggc agttccgtct gatttcccga ctgccgcggc agccgttaca
     2401 aggaccggct tcagggaaac aagtaccgct gaggccttca ggatgatggc cgcttacgat
     2461 ataggcaaaa gcaccaggta ttatacccat atccgggaag agatccgcaa agccggcggg
     2521 gatcagaaaa tactttccgg acttgagagg gaaatggtga aaattctgaa cagttccgcg
     2581 gccaccgcag aggccaagaa gctcatgctc cgcgaactgg gctggatggg atcggattat
     2641 tgtgtcaatg ctgtaaaacg gcttattgaa gttcctgagc tgaaggagga ggcagaatct
     2701 gcacttgaga ggctcgggaa ataaccgcct gcttatgttt tccctggtaa tacccgtgtt
     2761 caatgaggaa aaactgattg atgagctggt aagacgtacc ataccagcca ttgaatcatt
     2821 tactgacgac tatgaggtga tttttgtcga tgacggaagt acggaccgca gtgccggaat
     2881 gatcctgggt tacagggaaa aaaacaaaaa gataaaactc ctttcacttt ccaggaattt
     2941 cggtcatcag gcagcctata ccgccggtct ggaatactcc tcaggtgata ttgcgggaat
     3001 gatggatggt gaccttcagg atccgcccga gatactgagg gatatgtaca ataagatgaa
     3061 agaggaggat ctggatgtag tgtcgggaat aagaagcggc cggaaaggga gcaggggacg
     3121 taatttaaca tcccgaacct ttcatgccct gttcagacat gcgggagatc tggcgaacat
     3181 tgagaattcc ggcaatttct caataatgaa aaggattgtg gttgatgccg ttctgtcaat
     3241 gagggaaaag gtgcgctgcc ttcccggtct gagggcattt gccggcttca ggcagggata
     3301 tgtggaatat gtgagggatg gccgttacgg aggcagacca aaaatgacat tcgccaatct
     3361 gttcacactg gctggtgatg caatattttc attttcaaaa ttgcctgtca ggatctgtct
     3421 tattctggga atgacaggaa cggtggtctt tctgggggct ggcatctacc tgcttatcgc
     3481 cagggctgcg ggggcagcag ttcccggatg gtcgcccgcc ctgctcagtc tttatttcct
     3541 ggggtcagta cagcttgtgt tccttggaat actgggagaa tacatctaca gaaactacaa
     3601 ggaatcccag aaccgtccca tttattttgc aaggaagttc tatgatgaat atgatggaaa
     3661 tgaagaggaa ggatgaaaga cccatatgga aaagaatctt cttcattgcc tcggcacttt
     3721 atctgatcct gatgctgttc ctgagcaggg atgccggaat atcatgcgat gacacccttc
     3781 attatgacca ttcggttgat gtttataatt actttgccac tcacggcagc gacaggtcgg
     3841 cgctggaaac acccgttaca cacctgaaat attacggcc
//
LOCUS       DAOSPX010000315         3849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_21309_length_3849_cov_4.230364, whole genome shotgun sequence.
ACCESSION   DAOSPX010000315 DAOSPX010000000
VERSION     DAOSPX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21309_length_3849_cov_4.230364"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLZ75_12315"
     CDS             <1..684
                     /locus_tag="PLZ75_12315"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOS73219.1"
                     /translation="ELSVESLPLFISSIPDLKSLLKKPVSIFYNVISQADRRLSEIRD
                     KKPEKRKIFIVCGPRGKGKTSFVRKVIELLRGQNLQVSGIIAEALTDGPESGGYDIVN
                     LETGERMAFMRKEDKYGNDRIGRFYICPEGLEGGKALFAGLVSAKTDIVVIDEAGRLE
                     LRGGGWCDSITDLLGKSEHNILMTVRREFVERIRNKWNLENVIVFDIAKTDPGKAVSV
                     IIKNIRPDK"
     gene            927..1295
                     /locus_tag="PLZ75_12320"
     CDS             927..1295
                     /locus_tag="PLZ75_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOS73220.1"
                     /translation="MSEQEKIVIISTTGPEFQEKATLPFVVATAAQTVDVKVIVILQA
                     SAVLLAKQGMAENVNAPGLMPLKQLMDTFLELGGELQVCSPCIKERNIGEGNLVEGAV
                     TIAAGTVVSEVLSAKAVLTY"
     gene            1334..1900
                     /locus_tag="PLZ75_12325"
     CDS             1334..1900
                     /locus_tag="PLZ75_12325"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOS73221.1"
                     /translation="MENDLNARLLELQSQIEMLKEKVETLESRKKDQLSIAIVSGDLD
                     KILAAMIISVAAAAMDTSVKLFFSFWALSALRDKKKKVEGKDFISKMFGFMLPRGKNR
                     LKLSKMNMCGMGPLMIRYLMKKQNVLSLEQMFREAGELGIEITVCEMSMNLMGFKPGE
                     IIDYPNLRFAGAATFVADAGESSIQLFI"
     gene            1981..2232
                     /locus_tag="PLZ75_12330"
     CDS             1981..2232
                     /locus_tag="PLZ75_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOS73222.1"
                     /translation="MTAEELKNLDVTKSVDARGTACPGPLLEAKKAIGTIKSGDIMEV
                     LSADEGTRVDIPKWCGKQGHEYLGTIEESGYYKILLRKK"
     gene            2235..2642
                     /locus_tag="PLZ75_12335"
     CDS             2235..2642
                     /locus_tag="PLZ75_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOS73223.1"
                     /translation="MPGPATKSARILVFSTEKISDPAIDMAGLLKLHYPPTVYTIAVP
                     CSAGIKPRWILHAYEKGFDGVFIAADGTDCSYGDSCTDRTGRLVYQTHELMKQKNIEP
                     ARLRMAAICSICSESFVKQIKSFNEYLLRNSIN"
     gene            2645..>3849
                     /locus_tag="PLZ75_12340"
     CDS             2645..>3849
                     /locus_tag="PLZ75_12340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS73224.1"
                     /translation="MSDEIRAASYDALVIGGGIAGGEAALDLANNGYKVLLVEKDLSI
                     GGKMILLSKVFPTLDCAACITTPKVSEISRHPGITVFTGAEVTSIEKKGEREFETAVT
                     KRPRYVVEEDCTACQLCEEVCPVVVHDEYQYRLVGRKAAFIPFSIASPRVAAIDIENC
                     TLCGACEKACPPKCIDFTQETEKYIIKTGTVIVATGFKLFDPLKIPRYGYGVYKNVLT
                     SMEMERQLAPTRPFNTILRPGDGKMPDRIAYVLCTGSRDDTIGNPVCSQICCMYSLKQ
                     AQLLMGALPMADVTIYYINIRSFGKGFEEFYEQAKGMGISFVKGKVGKITETETGNLI
                     LRYEDINENRVMEEEHDMVVLAVGVLPNTDPLSFFRNGELKADQFSFVDQAEVLSSPA
                     RTSVKGVFVA"
BASE COUNT         1148 a          774 c          984 g          943 t
ORIGIN      
        1 gaactctctg ttgaaagcct gccgctcttc atttcttcaa taccggacct gaaaagcctg
       61 ctgaaaaaac ccgtgtcaat tttttacaat gtgatttcac aggccgatcg cagactgtca
      121 gagatcaggg ataaaaaacc ggagaaaagg aaaattttca ttgtctgcgg ccccagggga
      181 aaaggaaaaa caagcttcgt gcggaaagtg attgagctgc tcaggggaca aaacctgcag
      241 gtgtcgggaa taattgcgga agccctgacg gatggcccgg aatcgggagg atatgatata
      301 gttaatctgg aaacagggga aagaatggcc ttcatgcgga aagaagacaa atacggaaat
      361 gacaggatag gaagatttta tatatgtccg gaaggcctgg aaggaggaaa ggcattgttt
      421 gccggtcttg tttcagccaa aacagatatt gtcgtaattg atgaggccgg acgccttgag
      481 ctgcgcgggg gaggctggtg tgattccatt actgaccttc tcggaaaatc tgaacacaac
      541 atactgatga cagtccgccg ggaatttgtt gaaaggataa gaaacaaatg gaatcttgaa
      601 aatgtgattg tctttgatat agcaaaaact gatcccggaa aggcggtttc tgtcattata
      661 aaaaacataa ggcctgataa gtagaactga cttcaggaaa tgaaagacat gaaatgatat
      721 ttcacagcct gaaccaagct gtatggaact tattgttatt tttgtaacac ttaacagaac
      781 agacgtgttg cctttaacta actgaaatac agctggcaaa caataattta gcaataataa
      841 aatattcctg aaaggaatta aaccatacct gagccggata gcagaaatac tcaattatta
      901 tattaaaagt taaaaatcag cttattatgt cagaacagga aaaaatcgtg ataataagta
      961 caacagggcc tgaatttcag gaaaaggcta cccttccctt tgtagtggct actgcggcac
     1021 agacggtaga cgttaaggta atagtaattc ttcaggcttc tgccgttctg ctggcaaagc
     1081 aggggatggc tgaaaatgtt aatgctcccg gtctgatgcc cctgaaacaa ctgatggata
     1141 cattcttaga actgggcgga gaactgcagg tctgttctcc ctgcataaag gaaagaaata
     1201 ttggcgaagg aaatcttgtt gaaggagcag tgacaattgc tgcgggtaca gtggtttcag
     1261 aagtattgtc cgccaaggca gttctgactt attaggtgta tgatgtttaa tctgactaaa
     1321 cagaaaaaaa gagatggaaa atgatttaaa tgccagactg cttgaactgc agtctcagat
     1381 cgaaatgctg aaagagaagg ttgaaaccct tgaaagccgg aaaaaggatc agctttcgat
     1441 agcgattgtt tccggggatc tggacaagat tcttgcggca atgattatat ccgttgctgc
     1501 cgcagcaatg gataccagtg taaaactgtt cttttcattc tgggcccttt cggcactgag
     1561 agacaagaaa aagaaggttg aggggaagga tttcatatcc aaaatgtttg gttttatgct
     1621 tcccaggggg aaaaacaggc tgaaactttc aaaaatgaat atgtgtggca tgggtcctct
     1681 gatgatcagg tatctgatga aaaagcagaa tgtactttcc cttgaacaaa tgttcaggga
     1741 agcaggagag ctcggcatcg agatcacagt atgtgaaatg tcaatgaatc tcatgggttt
     1801 taaacccggg gaaataattg attatcccaa tctgagattt gccggcgcgg ccacttttgt
     1861 agcagatgca ggtgaaagca gcattcagct gttcatttga tcagtcaggc agtatatcat
     1921 aaaatatttt ataaataacc ggtcgtactg taaataataa tcatttcaaa atttaaactt
     1981 atgacagcag aagaattaaa aaatctggac gtgacaaaat ccgttgatgc caggggaacc
     2041 gcatgccccg gccccttgct tgaagccaag aaggctatag gtacaattaa gtcgggtgac
     2101 ataatggagg ttctttccgc cgacgagggc accagggtgg atattccgaa atggtgtggc
     2161 aagcagggac atgagtacct gggaaccatt gaggaaagcg gttattacaa gatcttactc
     2221 aggaaaaaat agcaatgccg ggtcctgcta cgaaaagcgc caggatcctg gtgttttcca
     2281 cagaaaagat atccgatccg gccattgata tggcaggatt gcttaagctt cattatcccc
     2341 ccacggttta caccattgca gtcccgtgtt ccgcaggcat aaagccgcgg tggatactgc
     2401 atgcctacga aaagggcttt gatggtgtat tcattgctgc tgacgggaca gactgttcct
     2461 acggggattc atgtactgac aggaccggga ggcttgtata tcagacccat gagctgatga
     2521 aacagaaaaa cattgagcca gcccggctaa gaatggctgc tatatgttcc atatgcagcg
     2581 aatcttttgt aaagcagata aaaagcttta atgaatactt gctcaggaat tcaattaact
     2641 aattatgtca gatgaaataa gagctgcctc ctatgatgcc cttgtcatag gtggcggcat
     2701 tgccgggggc gaggctgcgc ttgatctggc caataacggg tacaaggtgc tccttgttga
     2761 aaaggatctt tcgataggag ggaaaatgat acttctcagc aaggtttttc ccactctgga
     2821 ctgtgctgcc tgcatcacca cccccaaggt ttcggagatt tcaagacatc ccgggatcac
     2881 cgtttttacc ggcgctgaag tcacttccat tgaaaagaag ggcgagaggg aatttgaaac
     2941 ggcagtaacc aaaaggcccc gctatgttgt tgaggaagac tgcacggcct gtcagctgtg
     3001 cgaggaggtc tgccccgtag tggttcatga tgaataccag tacaggctcg tgggaaggaa
     3061 ggctgccttt atccccttca gcatagccag ccccagagtt gcagcaatag atattgaaaa
     3121 ctgtacactc tgcggggcat gcgaaaaagc ctgtccgccc aaatgcatcg atttcacgca
     3181 ggaaacggag aaatacataa taaagaccgg tactgtcatt gttgcaacgg gatttaaact
     3241 ctttgacccc ctgaaaatac cgagatatgg ctatggggtt tacaagaatg tcctgacttc
     3301 catggagatg gaaagacagc tggcccccac aaggcccttc aataccattc tcaggcccgg
     3361 cgacggcaag atgcctgaca ggattgccta tgtcctctgt accgggtcac gtgacgatac
     3421 catcgggaat ccggtctgtt cacagatatg ctgcatgtat tccctgaaac aggcccagct
     3481 tctcatggga gctctgccca tggccgatgt cacgatatat tatattaata tcagatcatt
     3541 cgggaagggc tttgaagagt tttatgaaca ggcaaagggt atgggtatca gctttgtcaa
     3601 ggggaaggtg ggcaagatca ctgaaacgga aacagggaac ctgatcctca gatatgagga
     3661 tatcaacgaa aacagggtga tggaggagga gcacgacatg gtggttctgg ccgtgggagt
     3721 tctgccgaac actgacccac tgtcattttt ccggaacggg gagctgaagg cggatcagtt
     3781 cagcttcgtg gaccaggctg aagtcctttc aagtcctgcc agaacgagtg tcaagggtgt
     3841 ttttgttgc
//
LOCUS       DAOSPX010000316         3772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_21873_length_3772_cov_4.207425, whole genome shotgun sequence.
ACCESSION   DAOSPX010000316 DAOSPX010000000
VERSION     DAOSPX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21873_length_3772_cov_4.207425"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..715
                     /locus_tag="PLZ75_12345"
     CDS             56..715
                     /locus_tag="PLZ75_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOS73225.1"
                     /translation="MNNQEKEPLFSARNIKLLTAPLHKDNPITIQVLGICSALAITVK
                     LKPALVMALSVTIVVAISNVVIAALRSYIPDRIRIIVQLVVIATLVIMVDQILKAYAF
                     DVSKQLSVFVGLIITNCILMGRLEAFAMANRPWPSFLDGLGNGAGYGLILVIVAFFRE
                     LLGSGTIFGYPVMEKIGIYTTGYENNGMFILPPMALILVGLIIWAQRSKDRSLIEEKQ
                     S"
     gene            721..1338
                     /gene="nqrE"
                     /locus_tag="PLZ75_12350"
     CDS             721..1338
                     /gene="nqrE"
                     /locus_tag="PLZ75_12350"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOS73226.1"
                     /translation="MENLLNIFIKGIFVENMIFAFFLGMCSYLAVSKTLKTSFGLGIA
                     VVFVLVITVPVNFLIENYILKKGALAWLGKGFADVDLSFLSFIVFIAVIAAIVQLVEM
                     VIEKFSPALYNSLGIFLPLIAVNCAILGASLFMQEREYANIAEATAFGAGSGVGWLLA
                     IVGIAAIREKLHYSNVPDPLKGLGITFIITGLMAIAFMGFMGITL"
     gene            1355..2608
                     /gene="nqrF"
                     /locus_tag="PLZ75_12355"
     CDS             1355..2608
                     /gene="nqrF"
                     /locus_tag="PLZ75_12355"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOS73227.1"
                     /translation="MILAVSTIQTLLFALIVFMIIILLLVAMLLIARDRLMPKGDVQL
                     LVNDREMTVSPGSNLLTTLSSNGIFLPSACGGMGTCGMCKCRILEGGGSILATETGFF
                     TRKEQLNNWRLSCQVKIREPLKIEVPESVLGIRKWQCEVVSNKNVATFIKEFVVRLPD
                     GEELNFDPGGYIQIDVPKYSADYRDFAIEEEFHADWDNFKVWDFSIRNTEETFRAYSM
                     ANYPAEKNLIKLNIRIATPPWDNVRKQYLNVPPGICSSYIFSLKPGDKVMISGPYGEF
                     HIKDTDNEMVYIGGGAGMAPLRSHIFHLFHTLKTNRKVSYWYGARSLREVFYEEEFRE
                     IERRFPNFRFHLALSAPLPEDNWTGLTGNIHQVLLDNYLKMHDEPEDIEYYFCGPPVM
                     NDAVLNMLDNLGVPSENISFDDFGI"
     gene            2660..3676
                     /locus_tag="PLZ75_12360"
     CDS             2660..3676
                     /locus_tag="PLZ75_12360"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOS73228.1"
                     /translation="MNSLRLIILLTGILCFDSSCREKTEYSKFSGFAQGTTYSMIFEN
                     PRRFNVAAIQDEVEQILTGFDLSLSLYVDSSVLCRINRNETDIPDEYFTEVFRRSKEI
                     SALTGGAFDVTVGPLVRAWGFGPDAHKNFRETDRDSLMQLVGIEKVDIRNGRILKADP
                     RISLDFNAIAKGYSVDVISRFFDRKGIKSYLVEIGGEIRARGDRGGVFWKIGIDKPAD
                     SNVVPGQDLQAIISMKDRSLATSGNYRRFYMEDGVKYSHTIDPKTGYPSKNRLLSATI
                     LASDCMTADGIATACMVMGKERAIEFIERNPEFDAFLIYSDDQGNYRTWISEKLIQYI
                     DQSR"
BASE COUNT         1036 a          810 c          928 g          998 t
ORIGIN      
        1 ttaccttctg aaaaacagaa attgatattt aacaacacat aaataatatt ttcggatgaa
       61 caaccaggaa aaagagcctt tgttttcggc acgaaacatt aaactgctca cggcaccgct
      121 gcacaaggac aatccgatta ccatacaggt tctgggaatc tgttcggcac ttgccattac
      181 agtgaagctt aagccggccc ttgtcatggc attgtctgtt acaatagtcg ttgcaatatc
      241 caatgtggtg atagccgcat tgcgcagcta cattcccgac aggataagaa taattgttca
      301 gctggttgta atagcgaccc tggtgataat ggtcgaccag atactgaagg catatgcttt
      361 tgatgtaagc aagcagctgt ctgttttcgt gggactgatt atcacaaact gtatcctgat
      421 gggcaggctt gaagcctttg caatggcgaa caggccctgg ccctcttttc tcgacggcct
      481 gggcaatggc gccggctacg gactgatcct tgttattgta gcattcttca gggaattgct
      541 gggatcaggt acaatattcg gatatcccgt aatggagaaa atcggaatat acaccaccgg
      601 ctatgaaaat aacggaatgt ttatacttcc gcccatggcc ctgattctgg tgggactgat
      661 aatctgggcc cagagaagca aggacagaag tctgattgaa gaaaaacaaa gctagtattt
      721 atggaaaatc tgcttaatat atttataaaa ggcatctttg ttgaaaacat gatttttgcc
      781 tttttcctgg gcatgtgttc gtaccttgct gtgtcaaaga ccctgaaaac atcattcggc
      841 ctgggaatag cagtggtttt tgtactggtg ataaccgttc ccgtaaactt cctgattgaa
      901 aattacatcc tgaagaaggg agccctggca tggctgggaa aagggtttgc agatgtcgat
      961 ctcagctttc tcagctttat agtgttcatt gccgttattg ccgccatagt tcagcttgtt
     1021 gagatggtaa tagagaaatt cagtcctgca ctctataatt ctctgggtat ctttctccct
     1081 ctgattgccg tgaactgtgc aatacttggt gcttcacttt tcatgcagga aagggaatat
     1141 gcaaatattg ccgaggcaac agccttcggg gccggatcag gggttggctg gcttcttgcc
     1201 atagtaggaa tagcagccat aagggagaaa ctgcattaca gcaatgtgcc ggatcccctg
     1261 aaaggtcttg gaataacatt tattatcacc ggattaatgg caattgcctt catgggattc
     1321 atgggaatca ctttataatt gaaattttca gaatatgatt ttagctgttt caactatcca
     1381 gactctcctt tttgcactga ttgtcttcat gatcattatt ctcctgctgg ttgcaatgct
     1441 tctcattgcg agggacagac tgatgccgaa gggtgatgtt cagcttctgg tcaatgacag
     1501 ggaaatgact gtatctccgg gttcaaatct tcttacgaca ctctcgtcga acgggatttt
     1561 cctgccttca gcctgcgggg gaatgggtac atgcggaatg tgcaagtgcc gtatccttga
     1621 aggcggcgga tccatcctgg caaccgaaac aggctttttt acaagaaagg agcagcttaa
     1681 caactggcgt ctcagctgcc aggtcaagat aagggagccg ctgaaaatcg aagtccccga
     1741 atcggttctc gggatcagga aatggcagtg cgaagtggta tcgaacaaaa atgtcgcaac
     1801 atttatcaag gagtttgtag tcaggctgcc cgacggagag gaactgaact ttgatccggg
     1861 aggctatatc cagatagatg tcccgaaata ttcggcagat tacagggatt ttgccataga
     1921 ggaagagttc catgccgact gggataattt taaggtgtgg gatttcagta tcagaaatac
     1981 ggaagaaaca ttcagggctt attccatggc caattatccc gctgaaaaga acctgatcaa
     2041 gcttaatatc agaatagcca cccccccctg ggataatgtc aggaaacaat atctgaacgt
     2101 tcctccgggg atctgttcat cctatatatt ctcactgaag ccgggagaca aggtcatgat
     2161 ttcaggaccc tacggcgaat tccatatcaa ggacactgat aatgaaatgg tttatatagg
     2221 tggcggggca ggaatggctc cgctgcgctc acatatcttc cacctcttcc ataccctgaa
     2281 aacaaacagg aaggtttcat actggtatgg agccagatca ctcagggaag ttttctatga
     2341 agaggaattc cgggaaattg aaaggaggtt ccctaatttc agattccacc tggccctttc
     2401 ggcacccctg ccggaagaca actggacggg actcaccggc aatatccatc aggtactcct
     2461 ggataactat cttaaaatgc acgatgagcc cgaagatatt gaatattatt tctgcgggcc
     2521 gccggtgatg aatgatgcag tactgaacat gctggacaat cttggtgtgc cgtctgaaaa
     2581 catctctttt gacgacttcg ggatttaaaa caggggtaca cttacacact aacacccgca
     2641 agggaaaaaa cggattaaga tgaatagttt aaggctgata atcctgctta caggcattct
     2701 gtgtttcgat tcatcctgca gagagaagac ggaatattcg aagttcagcg gttttgcaca
     2761 gggtaccacc tacagtatga tatttgaaaa tccgcgccgc ttcaatgtgg cagcaattca
     2821 ggatgaggtg gaacagatac tgaccggatt tgacctgtca ctttccctct atgtggattc
     2881 ttccgttctt tgccgtatca accgcaatga aactgatatt ccggatgaat atttcaccga
     2941 ggttttcaga aggtcgaagg aaatatctgc attgacggga ggtgcatttg acgtaacagt
     3001 cgggccgctt gtaagggcat ggggtttcgg gcctgatgcc cataaaaatt tcagggaaac
     3061 agacagggac agcctgatgc agcttgtggg gattgaaaag gtagatatcc gcaatggcag
     3121 gattctcaag gctgatccga gaatatccct ggatttcaat gcaattgcca agggctattc
     3181 agtagatgtg ataagccggt tttttgaccg caagggaata aaaagctatc tggttgaaat
     3241 aggaggggag ataagagcca ggggcgacag gggaggagta ttctggaaaa tcggtattga
     3301 caagcctgcc gacagcaatg tggttccggg acaggacctt caggcgataa tcagcatgaa
     3361 ggacagatct cttgccactt ccggcaatta caggaggttt tatatggaag atggtgtaaa
     3421 atactcccat accattgatc cgaaaaccgg ttacccttcg aagaaccggc ttctaagcgc
     3481 aaccatcctt gcttccgact gcatgacagc cgacgggata gccacagcct gcatggtgat
     3541 gggcaaggaa agagcgattg agttcattga acgcaatcct gaatttgatg cctttcttat
     3601 ttattctgat gatcagggaa actacaggac ctggatatca gaaaaactta ttcagtatat
     3661 agatcaatcc aggtagggta actgtcagca ggagctgcat atcctcccgt tgccgcaggg
     3721 cacgcagcct gtgtccacat gccttgtaca ctttattccc agtttgcgca tt
//
LOCUS       DAOSPX010000317         3759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_21968_length_3759_cov_4.595842, whole genome shotgun sequence.
ACCESSION   DAOSPX010000317 DAOSPX010000000
VERSION     DAOSPX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21968_length_3759_cov_4.595842"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLZ75_12365"
     CDS             <1..667
                     /locus_tag="PLZ75_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS73229.1"
                     /translation="ESGKWDFAPEYGLARSGLFALQDHGSRIWFKNLKLKELPTKPKE
                     EILFNGKDLTGWDVYGTELWYVKDGELICESGPDKDYGYLGTRKYYDDFDLSVDFKQV
                     SDGNSGIFIRSYIKTGVIISGWQVEVAPPGHHTGGIYESYGRGWIALIPDEKQSILKM
                     GEWNTMRIKMQGDVITTWLNGQEMTVLEDEKIGRGKGRILLQIHDGGGIKVCWKNFVL
                     KEL"
     gene            complement(783..2711)
                     /locus_tag="PLZ75_12370"
     CDS             complement(783..2711)
                     /locus_tag="PLZ75_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOS73230.1"
                     /translation="MNTEAYRQILTRYWGFTSFKPLQEEIILSVAGGRDTLALMPTGG
                     GKSITFQVPALHMEGICVVITPLIALMKDQVRRLNSLEIKAMAIHSGMSGEEIDIALD
                     NCIYGDYKFLYISPERISTRLFQERVSRLNINLVAVDEAHCISQWGYDFRPSYLKIAA
                     LRQLIPDRIPFLALTATATPAVAGDIMEKLEFREKNILRTSFGRKNISYMVKRVEDKA
                     AWLTNTLRNIGGSGIIYVRSRRRCKEIAEMLTANKFSADFYHAGLPQDIRDRKQSAWT
                     AGSTRIIVATNAFGMGIDKAEVRFVIHWEMPDSIEEYFQESGRAGRDGKKAFAILLYS
                     SSDRQQLESSLKKKFPPVGKIKDVYEALCNYLQIPVGSGKNTVFDFSIRDFVSKYRLP
                     VIETFNSLSFLQREGYVEFTEEINNPSRVHFVVGRDDLYRFQVANEAFDDFIKLLLRS
                     YTGMFTEFVAIDEDSLARRSGLSRDAVYRFLVKLSSLNIIRYIPGKKTALVIFTEERL
                     ERKALVISSDNYLDVKDKHVFRLNKMIDYAESATRCRSELLLDYFGEESDRCGICDVC
                     RERNELDLSKYEFDIILDEVKKILTGNSYEAAELVSKVSLPPEKVIKVVRWLLDHNKI
                     VRDDNNLLSWREPDLFSR"
     gene            2831..>3759
                     /locus_tag="PLZ75_12375"
     CDS             2831..>3759
                     /locus_tag="PLZ75_12375"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOS73231.1"
                     /translation="MSFTNDKILFEGLTFDDVLLIPSYSEVLPREADLSSMFSRNIII
                     NTPLVSAAMDTVTEDDLAIAIAREGGIGVIHKNMSIEKQASQVRKVKRAENVMIFDPI
                     TMDPDATVADAVNLMNEYKIGGIPVIDKNGILKGIVTKRDLRFENNRRRKIGEVMTKD
                     LITTDENTNLEAAARILQRHKIEKLPMVDEKGKLKGLITFKDITKIKDRPNSCKDDKG
                     RLRVAGAVGIAPDTAERVEALVNANADALVIDTAHAHSANVLRMLREIKKNYPDIDVV
                     VGNIGTGEAARKLVDEGADAVKVGIGPGSICTTR"
BASE COUNT         1058 a          867 c          893 g          941 t
ORIGIN      
        1 ggaaagcggc aaatgggatt tcgctcctga atacggactg gcccgcagcg gacttttcgc
       61 actgcaggac catggcagca gaatatggtt caaaaatctg aaactcaagg aattgccgac
      121 aaaacccaaa gaagagattc tgtttaacgg caaggacctt accggatggg atgtctatgg
      181 tacggaatta tggtatgtaa aggacgggga actgatttgt gaaagcggtc ctgacaagga
      241 ttacggatac ctgggaacaa gaaagtacta tgatgatttc gatctgagcg tcgatttcaa
      301 acaggtgtct gacggcaaca gcggaatatt catccgttcc tacatcaaaa ccggggtgat
      361 catttccgga tggcaggtgg aggtggctcc tccggggcat catacgggcg gaatctatga
      421 atcctacgga agaggctgga ttgcacttat tcccgacgaa aaacaaagca tcctgaagat
      481 gggtgaatgg aacaccatgc gcataaaaat gcagggggat gtgattacaa cctggctcaa
      541 cggacaggag atgacagtac ttgaagatga aaagatcggc cggggaaaag gcaggatact
      601 gctgcagatt catgacggcg gaggaataaa ggtctgctgg aaaaatttcg tcctgaaaga
      661 actgtaacaa caacgccgcg ttaaatgcaa tttatcccgg ggggtctgta cccggtcagg
      721 gcaggttctg acgaaactgg cctcccgata gaagagaacc gtttagcgcg gcgtaaatat
      781 ttctatctgc tgaacagatc cggttcccgc caggaaagca ggttattgtc atcccttacg
      841 atcttgttgt ggtcgagcag ccatcttacg accttgatga ctttttccgg gggcaggctg
      901 actttgctca caagttccgc ggcttcatag ctgtttccgg tcaggatctt ctttacctca
      961 tccaggataa tgtcaaattc atatttgctg agatccagtt cattcctttc ccggcagacg
     1021 tcacatatcc cgcagcggtc tgattcctcc ccgaaataat ccagcagcaa ttctgaccgg
     1081 cacctggtgg cagactcagc atagtcgatc atcttattga gccggaaaac atgtttgtcc
     1141 tttacatcaa gatagttatc ggatgatata accagggctt ttctttcaag cctttcttca
     1201 gtaaatataa ccagtgccgt tttctttccc ggaatatagc ggataatgtt cagcgatgaa
     1261 agcttgacga gaaatctgta aacggcatcc cgggaaagtc cggatctcct ggccagtgaa
     1321 tcttcatcta tggcaacaaa ttccgtgaac attccagtgt acgacctcaa taagagttta
     1381 ataaaatcat caaaagcctc atttgcaacc tggaacctgt acaggtcatc cctgccgacc
     1441 acaaaatgca cacgcgaggg attatttatc tcctcggtga attcaacata gccttcacgc
     1501 tgaagaaaac tgagactgtt gaatgtttca ataaccggca gacggtattt tgaaacaaaa
     1561 tcccggatac tgaaatcaaa tacagtgttt tttcccgatc ctaccggaat ctggagataa
     1621 ttacagagag cctcgtaaac gtcttttatt tttccgacgg gcggaaactt ttttttcagc
     1681 gaactttcaa gctgttgcct gtcggaagat gaataaagca atattgcaaa tgcctttttc
     1741 ccgtccctcc cggccctgcc gctttcctga aaatattcct cgatggaatc gggcatttcc
     1801 cagtgaatca caaatctgac ctccgcctta tctataccca ttccaaaagc attggtggca
     1861 acaattattc ttgttgatcc agctgtccag gccgactgct tcctgtcgcg aatatcctgt
     1921 ggcagacccg catgataaaa gtctgcactg aacttatttg ccgtcagcat ttcggcaatt
     1981 tccttgcagc gcctgcggct cctcacgtat atgattccgc ttccaccgat attccgcagt
     2041 gtattggtca gccacgctgc cttatcctcc acccgcttca ccatatagga aatatttttt
     2101 cttccaaagc ttgttctgag aatattcttt tcccggaatt caagcttttc catgatatcc
     2161 ccggcaacgg caggagtcgc agtagcagtg agtgcaagga aaggaatcct gtcggggatt
     2221 aattgccgta atgcagctat tttcaggtag gatggccgga aatcataacc ccactgagaa
     2281 atacagtgag cctcatcaac agctacaaga ttgatgttca gacgcgaaac cctctcctgg
     2341 aacaaacggg tggaaattct ctccggcgaa atgtaaagga atttataatc tccgtaaata
     2401 cagttgtcaa gagcaatgtc aatttcctct cccgacattc ctgaatgaat tgccatcgct
     2461 tttatttcca gactgttaag tcttctgacc tgatccttca tcagcgcaat caggggcgtg
     2521 atgacaacac agatcccttc catatgcagg gcgggaacct gaaaggtaat ggacttgccg
     2581 ccgccggtgg gcataagggc cagtgtatcc cttccgcctg caaccgatag aataatttct
     2641 tcctgcaggg gcttgaagga agtaaaaccc cagtacctgg tcagaatctg tctgtatgct
     2701 tctgtattca tgcctccttg aacaaaaata ctaactcttg gccgattttt tacattccgg
     2761 cagataattg aatgttttat gagtgatgaa tttttagtaa tttgcgcggc aaaacttaat
     2821 agcctgcccg atgtccttta caaatgataa aattctgttt gaaggtctta cattcgatga
     2881 tgttctgtta ataccttcat actcggaagt acttcccagg gaagccgacc tgagttccat
     2941 gttttccaga aatataataa tcaacactcc tctggtttca gcggcgatgg ataccgttac
     3001 ggaggatgat ctggcaatcg cgattgcccg cgagggcggc ataggagtga ttcacaagaa
     3061 catgagcata gaaaagcagg cctcgcaggt gagaaaggta aaaagggccg aaaacgtaat
     3121 gatctttgat cccattacca tggatcctga tgccaccgtt gccgatgcag tgaatctgat
     3181 gaatgaatac aaaatcggcg gtattccggt gatcgacaaa aacggtattc tgaaaggaat
     3241 tgtgacgaaa agggatctgc gcttcgaaaa caacagaagg cggaaaatcg gcgaagtgat
     3301 gacaaaggat ctgataacaa ccgatgagaa caccaatctg gaagctgccg caaggatact
     3361 gcaaaggcat aagatagaaa agctcccgat ggttgatgaa aaaggaaaac ttaaagggct
     3421 gataacattc aaggatatca caaagatcaa ggaccgcccc aactcctgca aggatgacaa
     3481 ggggagacta agggtggctg gtgcggtcgg cattgctccg gatacggccg aaagggttga
     3541 agcacttgtc aatgcaaatg ccgatgcact tgttattgac actgctcatg cccattctgc
     3601 caatgtgctg agaatgctca gggaaataaa gaagaattat cccgatatcg acgtggttgt
     3661 gggaaatatc ggtacgggtg aagcagccag gaaactggtt gacgaagggg ctgatgcggt
     3721 taaagtcgga atcggtcccg gatcaatatg caccacccg
//
LOCUS       DAOSPX010000318         3704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_22401_length_3704_cov_4.968485, whole genome shotgun sequence.
ACCESSION   DAOSPX010000318 DAOSPX010000000
VERSION     DAOSPX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22401_length_3704_cov_4.968485"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PLZ75_12380"
     CDS             complement(<1..1141)
                     /locus_tag="PLZ75_12380"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOS73232.1"
                     /translation="MKSIQQLLKEKPWLGWIIFTATVLIVFLLGMLASSIVQRRAEAV
                     FAYTPEVTFSDFEPRNSLWGELFPREYDSYLKTADTTFRSKYNGSAMVDMLQESPRMV
                     VLWAGYLFSRDYGQGRGHYYAIEDIWNTLRTGAPGAGVTSPQPNTCWACKSPDVPRLM
                     NETGAAEFYRGTWETKGSEIINPIGCADCHEAKSMNLRISRPALIEAFESQGRDISKA
                     SHQEMRSLVCAQCHVEYYFNKKRTEGVDYLVFPWKNGTSLEEIEKYYDDIEFADWTHA
                     LSRAPMLKAQHPDYEVYLTGTHASRGVSCADCHMPFISEGGQKFTDHHLQSPLNNIAA
                     SCQVCHREETDKLVQDVYSRQDKIMENRIILETLLVRCHVEAKTAW"
     gene            complement(1155..1739)
                     /gene="nrfH"
                     /locus_tag="PLZ75_12385"
     CDS             complement(1155..1739)
                     /gene="nrfH"
                     /locus_tag="PLZ75_12385"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOS73233.1"
                     /translation="MAFLKKLVPPDNWKFPVIVTLAIFTGLALFVFKISNAPSYLGDK
                     PETCVNCHIMAPQYATWSHSSHREKTNCNECHVPQDNIFRKYFFKAKDGLRHATVFTL
                     RREPQVIFIKDEGRKVVHRNCIRCHEELLTDSKLLARTTETHTFRMERDCTDCHRETP
                     HGRVNSLSSVPWARVPLPGPIVPGWLRNMMKNKD"
     gene            complement(1771..2268)
                     /locus_tag="PLZ75_12390"
     CDS             complement(1771..2268)
                     /locus_tag="PLZ75_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS73234.1"
                     /translation="MSIVYRHIRKKDNLPLARLIRSVFREFGISRPGTVYTDPTTDDL
                     YSLFKEPGSVYWVAEEDGNIVGGCGIFPTAGLPPGCAELVKLYLVPSKRGQGTGYELM
                     RKSLRSARRLGYDQIYLESLPELSRAISLYKKAGFEFISAPLGNSGHYGCDIWMLKKF
                     RKRNP"
     gene            2416..>3704
                     /locus_tag="PLZ75_12395"
     CDS             2416..>3704
                     /locus_tag="PLZ75_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOS73235.1"
                     /translation="MKVASSFFSVFKACLATLILFYSAAESAGRDYLVTGTVTDQTGR
                     AVPDARVSMLAGSAEYAAVSGSDGSYSLRISGIYEPVPDLLKQGIPYPNPFLNSINIP
                     FIINSTGDIRLMIHNISGQKIREFFFPAVKAGSYQIIWDGRTDNGAPARQGLYIYTIV
                     FSGRSWSGRLVKYSSGSSLSAVSTLEQVQIPAAGPSAGGSYRVPVITMVSCRDYYPVR
                     LTDITIGRDTTINFEICASVAIPFNTTADFVAMHTGSAYRPLNLKGINLGSSPPGTFP
                     GEIAYAITEKVYEDWIDLIAQAGFNSIRVYTLHPPEFYEKLANYNQRNPGKPLLLFQG
                     IWLGEIEDGSVTSEYDLVQRTNAFRSEIHEVIDCIHGKREIAFRLGRAYGSYRTDLSR
                     WTAGYIIGREIMPQEVDTTNRLHPSMTSFTGSQLSISG"
BASE COUNT          891 a          886 c          905 g         1022 t
ORIGIN      
        1 cccatgctgt tttagcctca acatggcatc tgaccagcaa cgtttccagg atgattctgt
       61 tttccatgat cttgtcctgc cttgaatata catcctgaac aagtttgtcg gtttcttcac
      121 ggtggcagac ctgacatgac gcagcaatgt tgttcagcgg gctctggagg tgatgatccg
      181 taaatttctg tcctccttca ctgatgaagg gcatgtggca gtcggcacat gacacgcccc
      241 ttgaagcatg ggttcctgtc aggtaaacct catagtcggg atgctgtgcc ttaagcatgg
      301 gagcgcggct cagggcgtgg gtccagtcgg caaattcaat atcgtcgtaa tacttttcta
      361 tttcttccag ggaggttccg ttcttccagg ggaatacaag gtaatccacg ccttctgtcc
      421 ttttcttgtt gaaatagtat tcaacatggc attgtgcaca gaccaggctt ctcatttcct
      481 gatgacttgc cttcgagata tcgcgtccct gtgattcgaa tgcttcgata agggctggcc
      541 gggatattct gagattcatt gattttgcct catggcagtc agcacatcct atcggattga
      601 taatctcact gcctttggtt tcccatgttc ccctgtaaaa ctctgctgct ccggtttcat
      661 tcatgagtct cggaacatcc ggactcttgc aggcccagca ggtattgggc tgcggactcg
      721 taacacctgc tccgggagct cctgtcctga gggtattcca tatatcttca atagcgtaat
      781 aatgacccct gccctgacca taatcccttg aaaacaggta tcctgcccag agcaccacca
      841 tccgcggtga ttcctgaagc atgtcaacca ttgcactgcc gttgtacttg cttctgaaag
      901 tggtatcggc cgttttgagg tatgagtcat attcccgcgg gaacagttcg ccccacagtg
      961 aattcctggg ttcaaaatca gagaaggtca cctccggggt ataggcaaac accgcttcag
     1021 ccctgcgctg cacaatggac gatgcaagca tgccaagcag gaaaacaatt aataccgtgg
     1081 cagtgaaaat aatccatcct aaccatggtt tttccttgag taactgttgt attgacttca
     1141 tacctgtgaa ttatttaatc tttgtttttc atcatattcc tgagccatcc gggtacgatg
     1201 ggccccggaa ggggaaccct tgcccaaggt acgcttgaga ggctgttcac ccttccgtgg
     1261 ggggtctccc tgtggcagtc cgtgcagtcc ctttccatcc tgaaggtgtg agtttcagtg
     1321 gttcttgcaa gcagtttgct gtcggtgaga agctcttcat ggcacctgat acagttcctg
     1381 tgtaccactt tccggccctc gtcctttata aaaatgacct ggggctccct gcggagcgtg
     1441 aaaactgttg catgccgcaa gccgtccttg gccttgaaaa aatatttgcg gaaaatatta
     1501 tcctggggta catggcattc attgcaattg gttttttcgc ggtgagagct gtgagaccag
     1561 gttgcatact ggggtgccat aatatggcag ttgacacagg tttccggctt gtcgcccaga
     1621 taggaagggg catttgaaat cttaaaaacg aaaagcgcca gtccggtgaa tatggcaagt
     1681 gtaacaatta cagggaattt ccagttgtcc ggaggtacaa gctttttcag gaatgccatt
     1741 atttttttag ccgcaatata ttaaaaatta ttatggattt cttttcctga attttttcag
     1801 catccagata tcacatccgt aatgccccga atttccaagg ggtgcagaaa taaattcaaa
     1861 tcctgctttt ttatacagac ttatggccct ggataattca ggaagggatt cgagatatat
     1921 ctggtcatag cccagccgtc tggctgatct caggcttttc ctcatcaatt catagcctgt
     1981 tccctgacct ctttttgaag ggacgagata tagtttcacc aattcggcac atccgggagg
     2041 cagaccggca gtggggaaaa taccgcagcc tccgactata tttccatcct cttctgccac
     2101 ccagtaaact gatccgggtt ctttgaacag ggaatagaga tcatccgtgg tgggatcggt
     2161 ataaaccgtt ccggggcgcg agattccgaa ttccctgaat accgatcgta tcagccgggc
     2221 aagcggcaga ttatcctttt tcctgatatg cctgtaaact atgctcaatg caaaaattat
     2281 cctgttcagc cgcaaagtta aaaaagcaga ctgaatttca tgaattataa acataagtag
     2341 cacggatgat tgtaattatg tatcttttta ttagatttga attctggatt aattattttg
     2401 aataccctcc tgaatgtgaa agttgcaagt agtttttttt ctgttttcaa agcatgtctt
     2461 gccactctga ttctttttta ctctgcggcc gagtcagcag gccgggatta ccttgttacc
     2521 ggaacggtta ctgatcagac cggaagggcg gttccggatg ccagagtctc catgctggca
     2581 ggcagtgctg aatatgctgc ggtgagtggc agcgacggca gctattccct gaggatttcg
     2641 ggaatctatg agcctgtacc cgaccttctg aagcagggga ttccctatcc aaaccctttc
     2701 ctgaattcca taaacatacc ttttatcatt aacagtaccg gtgacatacg gctgatgatc
     2761 cataacatat cagggcagaa aatcagggaa ttcttctttc ccgcggtgaa ggcgggcagt
     2821 tatcagataa tatgggatgg aaggactgat aacggagcac ctgcacggca gggcctgtat
     2881 atttacacca ttgtattttc cggcagatca tggtcaggcc gccttgtgaa atattcctca
     2941 ggttcatccc tgtcggcggt atcaaccctt gaacaggtgc agatacccgc agctggccct
     3001 tccgcgggcg gttcatacag ggtgccggtg atcaccatgg tcagctgcag ggattattac
     3061 ccggtgaggc ttacagatat tacgataggc cgtgatacaa cgataaattt tgaaatatgt
     3121 gcttcggttg cgataccttt caataccact gctgattttg tcgccatgca taccgggtct
     3181 gcttaccggc cactgaacct gaagggtata aatctgggat cctcgccccc cgggactttc
     3241 ccgggcgaga tagcctatgc cataacggaa aaggtgtatg aggactggat agatttgata
     3301 gcccaggcag gattcaacag tataagggtt tacacactgc atccaccgga attctatgag
     3361 aagctggcta attacaatca gaggaatccg ggcaaaccgc tcctgctttt tcagggtatc
     3421 tggctgggag agatagagga tggctccgtt acgtcggaat atgaccttgt tcagagaaca
     3481 aatgcattcc gcagcgagat tcatgaggtg attgactgta tacacggaaa aagggagatc
     3541 gccttcaggc tgggacgggc atacggcagc taccgcaccg acctgtcgcg ttggacagcg
     3601 ggttatatca tcgggaggga gataatgccc caggaggttg ataccacgaa caggcttcat
     3661 ccatcaatga catcttttac cggcagccag ctgagtattt cggg
//
LOCUS       DAOSPX010000319         3688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_22523_length_3688_cov_4.807872, whole genome shotgun sequence.
ACCESSION   DAOSPX010000319 DAOSPX010000000
VERSION     DAOSPX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22523_length_3688_cov_4.807872"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PLZ75_12400"
     CDS             complement(<1..872)
                     /locus_tag="PLZ75_12400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOS73236.1"
                     /translation="MKRVKVTLLLFVLIGITTWSQQDPLSSNYMFSTLTYNPGVAGTS
                     GMICATAIARQQWVGFTGAPATTTFNVSAAVSPFRVESGIGLMVESDQVGFDSDINIS
                     GIYALLVNMGTGKLGIGVNLGVMNKTLDPEWHIPSGDGFTPPDQDPLIPVNKESFVVF
                     DAGFGLYYNADNYYAGLSVRHLNQARFRFTKGEPYLTRHYYLTAGYNLQLPNPALELL
                     PSVFAFSDGRALQASVTTMLRYNKKVWGGVSYRAGDALTGIAGIELFNGIRIGYAYDF
                     PVSDIRKNTMGSHEL"
     gene            complement(1041..2213)
                     /locus_tag="PLZ75_12405"
     CDS             complement(1041..2213)
                     /locus_tag="PLZ75_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOS73237.1"
                     /translation="MTDLNDFFDPVSIGSPPEFEHMAGQSGFPHNIRVHTENLPISGL
                     ESFKIALLGVPEGRNSPASGSMKGPDCIRAELYRLAKIPGKLRIADLGNMKRGTTFND
                     TVAGLTEIIIMLAGYNVFPVILGGSSALAPAVDRALSRQQIRYTLLAVDSRIDYVSEK
                     KGNDSFSYLSTIMHDSRSTFSHYINLGYQTYLNDQQVINRFNRRRSELVRIGDVRKAI
                     HLTEPMFRDSDAVLMDISSVRQSDAPGCTAPSPNGFYGEEICLLARYAGISDNLKIFG
                     LFDLDPDLDIRNQTAALCAQILWFFLEGFSQKQYETPHLSSANSGRFIRYHVRISDLD
                     EDLIFVKSNLTERWWMELKGPASSPLYIACSHEDYIKASRDEVPERWVRGSKRLRP"
     gene            complement(2232..>3688)
                     /locus_tag="PLZ75_12410"
     CDS             complement(2232..>3688)
                     /locus_tag="PLZ75_12410"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOS73238.1"
                     /translation="EAGKITYMRTDSTNLSKLALATLRETIISEYGQEYSKTRQYATR
                     TKGAQEAHEAIRPAYPANPSIQGSQNEKKLYDLIWKRTIASQMADALVEKTTITVGMD
                     NSPVKFTATGEVVKFHGFLKVYAESSDQENGDEEKNLLPPVKKDMPLYYNVVSAVQKF
                     TMPLPRYNEASLVKKLEELGIGRPSTYAPTISTIQNRGYVLKEDRPGEKRQISVYTLE
                     KGKISHSVKNEISGKEKSKLFPQDIGMIVNDFLVENFPEIVDYNFTADIERQFDEIAL
                     GKLKWTGMLKKFYAPFHKTVESTLEKKHRKAAVRFLGNHPETGEPVTVRMGRFGPVAQ
                     IGSSEDGNKPRYASLTHDQLIETISLEEALRLFTLPRTLGSFENEEVVVGKGKFGPYI
                     RHKGKFYSVKKGVDDPYSLTMERAIQIINEKNEAEKKKVVREFGDILLLNGRYGLYLS
                     KDKKNYRIPKGTDTDNLTREECEAIIERSEKKKK"
BASE COUNT          823 a          929 c          842 g         1094 t
ORIGIN      
        1 agttcatggg agcccatggt attcttcctg atatccgata ccgggaagtc ataggcatag
       61 ccgatacgta ttccgttaaa caattcaatg cccgcaattc ctgtcagggc atcacccgca
      121 cggtaagaaa cgccgcccca tactttttta ttgtaccgga gcatggtggt cacactggcc
      181 tgcaatgccc ttccgtcgct gaaagcgaat accgacggca acagctcaag ggccggattg
      241 ggcagctgca ggttatatcc tgctgtcagg taataatgcc ttgtgagata gggttctcct
      301 tttgtaaacc tgaatctggc ctggttcaga tgcctcaccg ataagccggc ataataatta
      361 tcggcattgt aatacaggcc gaatccggca tcaaaaacca caaagctctc cttgttcacg
      421 ggtatcagcg gatcctggtc gggcggggtg aatccgtcac cggaaggaat atgccattcc
      481 ggatcaaggg tcttgttcat gaccccgagg tttactccta ttcccagctt tcctgttccc
      541 atgttgacaa gcaatgcata tattcctgaa atatttatat cagagtcaaa tcccacctgg
      601 tcactttcca ccatcaggcc tattccgctt tctaccctga agggggaaac cgctgcactc
      661 acattgaagg tggttgttgc aggagcaccg gtaaatccca cccattgctg cctggcaatg
      721 gcagtggcac aaatcattcc tgatgtgccg gccactcccg ggttgtaggt aagtgtgctg
      781 aacatatagt tgctggacaa cggatcctgc tgcgaccacg ttgtaatgcc tatcagcaca
      841 aataacagca atgttacctt aacgcgtttc attttatgat gatccaacag tagagatgct
      901 cgtgcaaatt aaaaagcaaa ataaacaaaa atctgacacg tatacaaata tattgaataa
      961 cgcatattaa caaattttgt tatttaacgc acaataacaa tttttgtttc aatgagaagc
     1021 ccttaacttt ctgctttttt tcacgggcgc agccttttgg aaccccttac ccatctttcg
     1081 ggtacctcgt cgcggctggc cttgatgtaa tcttcatggg aacacgcaat atacagtggg
     1141 gacgaagcgg ggcccttcag ttccatccac catctttccg tgagattgct tttgacaaat
     1201 atcagatctt catccaggtc gcttatcctg acatggtatc ttatgaacct gccgctgttg
     1261 gcagagctca gatggggtgt ttcatactgt ttctgggaga atccctcgag gaagaaccat
     1321 aatatctgtg cacagagtgc tgctgtctga tttcttatgt ccagatccgg atccaggtca
     1381 aagaggccga atattttcag gttatccgat attccggcat atcttgcaag aagacagatc
     1441 tcctcgccat agaacccgtt gggagagggg gccgtgcatc cgggggcatc cgactgcctc
     1501 accgatgaga tatccatcaa tacggcatcc gaatccctga acatgggttc ggtaaggtgt
     1561 atggcctttc ttacatcacc tattcttacc agttccgacc tgcgcctgtt gaatctgttt
     1621 atcacctgct ggtcgttcag gtatgtctga tagcccaggt ttatgtagtg gctgaaggta
     1681 ctccggctgt cgtgcatgat ggtgctgagg taactgaagg aatcattccc cttcttctcg
     1741 ctgacatagt ctatcctgga atcaacggcg agcagggtgt atcttatctg ctgccggctc
     1801 agtgccctgt caacagcagg tgccagtgcg ctgctgccac caagtatgac cgggaaaaca
     1861 ttgtagccgg caagcatgat gattatttcc gtaagtccgg ccactgtatc attgaaggtg
     1921 gttccccttt tcatgttccc caggtcggct atcctgagct tgcccgggat cttcgccagc
     1981 ctgtagagtt cagcacgtat gcagtcaggc cccttcattg atccgcttgc cggggaattc
     2041 ctcccttcgg gaacacccag cagggctatt ttgaaggatt ccaatccgct gatggggaga
     2101 ttttcggtgt ggacccttat attgtgagga aatccggact ggcccgccat gtgttcaaac
     2161 tccggcgggc tgcctatact gacgggatca aagaaatcgt tgagatcggt catgatattg
     2221 ttttaggaag tctatttttt ctttttttcg ctcctttcaa taatggcttc acattcctcc
     2281 cttgtcagat tgtcagtgtc ggtgcccttt ggaatcctgt agtttttctt gtccttcgac
     2341 aggtatagcc cgtatctccc gttgagaagc aggatatcgc cgaattccct tacaaccttt
     2401 ttcttttccg cctcattctt ttcattgatt atctgaatcg ccctttccat ggttaatgaa
     2461 taggggtcat ccaccccctt ttttaccgag tagaatttgc ctttatgtct gatatagggt
     2521 ccgaattttc cttttccgac aacaacttct tcattttcaa aagacccgag agttctgggc
     2581 agggtgaaaa gtctgagggc ctcctcaagg cttattgttt caataagctg gtcgtgcgtg
     2641 aggctggcat aacgcggttt gtttccgtct tcgctgcttc ctatctgggc aaccggtccg
     2701 aacctcccca ttcttacagt aaccggctcc cctgtttcag gatggtttcc aagaaatctc
     2761 acagccgcct tccggtgctt cttctcaaga gtgctctcca cggttttatg gaaaggggcg
     2821 tagaattttt tcagcatgcc cgtccatttc agctttccga gagcaatctc atcaaactgt
     2881 ctttcaatat cagcagtaaa attgtaatcc actatttcgg gaaaattttc aaccaggaaa
     2941 tcatttacaa tcattcctat atcctgggga aagagtttcg acttctcctt tccgcttatt
     3001 tcatttttta cggaatggct tatttttcct ttttccaggg tatagacgct gatctgccgt
     3061 ttttcccccg gcctgtcttc cttcagcaca taacccctgt tctgtattgt cgagattgtg
     3121 ggagcgtatg tagaaggcct gccgattccc agctcctcaa gtttcttgac gagactggcc
     3181 tcattgtatc tcgggagcgg catcgtgaat ttctgaaccg ctgataccac attgtaataa
     3241 agcggcatat cctttttcac aggcggaagg agattctttt cctcatctcc gttttcctgg
     3301 tccgatgatt ccgcatatac tttcagaaag ccgtggaatt tgacaacttc gcccgttgca
     3361 gtgaatttga ccggcgaatt gtccatgcca accgtaatag tggttttttc aacaagcgca
     3421 tcagccatct gcgaggcgat ggtccttttc catatcaggt cgtagagctt tttttcattc
     3481 tgactgccct gtattgaggg attggccgga tatgcagggc ggatggcctc atgagcttcc
     3541 tgggcacctt tcgttctggt ggcgtactgt cttgttttcg aatattcctg tccgtattcc
     3601 gatatgattg tctcccgcag tgtagccagg gcaagttttg aaaggtttgt ggaatcggtt
     3661 ctcatatagg tgattttccc ggcttcgt
//
LOCUS       DAOSPX010000320         3682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_22580_length_3682_cov_3.838985, whole genome shotgun sequence.
ACCESSION   DAOSPX010000320 DAOSPX010000000
VERSION     DAOSPX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22580_length_3682_cov_3.838985"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="PLZ75_12415"
     CDS             <1..107
                     /locus_tag="PLZ75_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HOS73239.1"
                     /translation="TNQDEVRGTRIFGPVARELRDKYMKIVSLAPEVL"
     gene            127..450
                     /gene="rplX"
                     /locus_tag="PLZ75_12420"
     CDS             127..450
                     /gene="rplX"
                     /locus_tag="PLZ75_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOS73240.1"
                     /translation="MHKKLHVRKGDVVMVITGESKGQKGRVLGVDRKKDRAIVEGLNL
                     ISKHTKPHSKAPQGGIIRKEAPIHISNLMLIDPASGKPTRTGKRLNKKDKLVRYSKKS
                     GEEIK"
     gene            450..1001
                     /gene="rplE"
                     /locus_tag="PLZ75_12425"
     CDS             450..1001
                     /gene="rplE"
                     /locus_tag="PLZ75_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOS73241.1"
                     /translation="MYIPALKTKYNKEIVPALVKEFGYKTVMQVPKLRKIVINQGIGQ
                     AIADKKLLESGVKELTDIAGQKAVLTYSTKDVSNFKLRKNMPIGVMVTLRSERMYEFL
                     ERLISVALPRIRDFKGINARLDGRGNYTLGITEQIIFPEIDLDKVSKIMGLQVTFVTS
                     ANTDEEALALLRNFGLPFKTSKN"
     gene            1006..1275
                     /gene="rpsN"
                     /locus_tag="PLZ75_12430"
     CDS             1006..1275
                     /gene="rpsN"
                     /locus_tag="PLZ75_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOS73242.1"
                     /translation="MAKESMVAREVKRERMARKYAEKRKRLKAEGNYVALSRLPRNSN
                     PNRQRNRCKITGRTRGYMRQFGISRITFREMASKGLIPGVKKASW"
     gene            1312..1710
                     /gene="rpsH"
                     /locus_tag="PLZ75_12435"
     CDS             1312..1710
                     /gene="rpsH"
                     /locus_tag="PLZ75_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOS73243.1"
                     /translation="MTDPIADLLTRIRNAIQAGHKVVEAPASNLKKEIARILYEKGYI
                     LSYRVVDGVNTQGSLKIALKYNPKSKQPAIKSLQRVSRPGLRHYAAADKMPRVLNGLG
                     IAIISTSRGLMTDKEARKENIGGEVLCYVY"
     gene            1726..2280
                     /gene="rplF"
                     /locus_tag="PLZ75_12440"
     CDS             1726..2280
                     /gene="rplF"
                     /locus_tag="PLZ75_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOS73244.1"
                     /translation="MSRIGKMPVSLPAGVSVEISGNNVITVKGPLGQLSKPVDSDIEI
                     EVSDNEIQVKRPSESKHHKSLHGLYRALIANMVTGVSEGFKKELELVGVGYRAEARGQ
                     DLELSLGYSHDIIFRLPDEVKVETKTERRSNPIITLTSADKQLIGHVAAKIRSLRPPE
                     PFKGKGIKFVGEELRRKAGKSASV"
     gene            2310..2669
                     /gene="rplR"
                     /locus_tag="PLZ75_12445"
     CDS             2310..2669
                     /gene="rplR"
                     /locus_tag="PLZ75_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOS73245.1"
                     /translation="MALSKLDRRKRIRMRIRKKINGTAEMPRVAVFRSNKQIYCQLID
                     DQNHVTLLSASSKEKEIASETGIKKTEQAKLVGKLLAEKCRDKGIEQVVFDRSGYKYH
                     GRVKSLADAAREGGLKF"
     gene            2679..3194
                     /gene="rpsE"
                     /locus_tag="PLZ75_12450"
     CDS             2679..3194
                     /gene="rpsE"
                     /locus_tag="PLZ75_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOS73246.1"
                     /translation="MADSIRKVKTSDLELKDRLVSIQRVTKVTKGGRTFSFSAIVVVG
                     NEAGIVGHGLGKAGEVTTAIAKGIEDAKKNLIKVPVIKGTVPHEQEAKYGGARVFIKP
                     ASEGTGVKAGGAMRAVLESVGIKDVLAKSKGSSNPHNLVKATIAALLEMRDAYSVAGH
                     RGIPVQRVFNG"
     gene            3211..3390
                     /gene="rpmD"
                     /locus_tag="PLZ75_12455"
     CDS             3211..3390
                     /gene="rpmD"
                     /locus_tag="PLZ75_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOS73247.1"
                     /translation="MAKIRITQVKSRIGKPENQKRTLDALGIRKLNHSVEHEATPQIL
                     GMVNKIKHLVRVENI"
     gene            3417..>3682
                     /gene="rplO"
                     /locus_tag="PLZ75_12460"
     CDS             3417..>3682
                     /gene="rplO"
                     /locus_tag="PLZ75_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOS73248.1"
                     /translation="MDLSNLKPAEGSVRKGRRLGRGQGSGRGGTSTRGHKGAKSRSGY
                     SKRIGFEGGQMPLQRRVPKYGFTNINRREYKGINIGMLQTLSER"
BASE COUNT         1205 a          705 c          990 g          782 t
ORIGIN      
        1 ttacaaacca ggatgaggtg agaggtaccc gtattttcgg acctgttgca cgcgagctgc
       61 gtgataagta catgaaaata gtatcactgg cgcctgaagt attgtaggac ctgtaaacaa
      121 acagcaatgc ataagaaatt acatgtaaga aaaggtgatg tggttatggt gataaccggt
      181 gagtcgaaag gtcagaaggg ccgcgtccta ggagtcgaca gaaaaaagga cagggctatc
      241 gtggaaggcc tgaacctgat atcaaagcat accaagccgc attcaaaagc tccccaggga
      301 ggcattatca ggaaggaagc cccgatacat atttcaaatc tgatgcttat tgatcccgca
      361 tcgggaaaac caacccgtac aggaaagaga ctgaacaaga aagataaatt ggtacgttat
      421 tcaaaaaaat caggagagga gattaaataa tgtatatacc agctttaaaa acgaaatata
      481 acaaggagat cgttccggca cttgtgaagg aattcggtta caaaaccgtg atgcaggttc
      541 cgaaactccg gaaaatcgtt attaaccagg gtatcggcca ggccattgca gacaagaaac
      601 tgctggaatc aggagtaaag gaactgacag acatagccgg tcagaaagca gtcctgacat
      661 actccacaaa ggacgtttca aacttcaaac tgaggaagaa catgcctatc ggggtaatgg
      721 ttaccctcag aagcgaacgg atgtacgaat tcctcgaacg ccttatctcg gttgccctgc
      781 cgcgtatacg tgacttcaag ggaattaatg ccagacttga cggaaggggg aactataccc
      841 tgggaattac ggaacaaatc atcttccccg agattgatct ggataaggtg agcaagataa
      901 tgggtttgca ggtcactttc gtgacctcgg ccaacacaga tgaagaggca ctggcccttc
      961 tcaggaattt cggattaccg tttaaaactt caaaaaactg attatatggc taaggaatca
     1021 atggttgccc gtgaggtaaa gcgtgaaaga atggcccgga aatatgctga aaagagaaaa
     1081 agactcaagg ctgaaggcaa ttatgtggca ctgagtcgtc tgcccagaaa ctcaaatccc
     1141 aacaggcagc gcaaccgctg taaaatcaca ggccgtacca ggggatatat gaggcagttc
     1201 ggaatcagca ggataacttt cagggaaatg gcttccaagg gtttgatacc gggtgtaaag
     1261 aaagcaagct ggtaatataa attgaataaa aactttacaa aataacagaa gatgactgat
     1321 ccgattgcag atttattgac cagaatcaga aatgccatcc aggctggtca caaggttgtc
     1381 gaagcaccgg catccaatct gaagaaagag atagcgagga tactctatga aaagggatat
     1441 attctgagtt acagggtggt ggacggggtg aatacccagg gaagccttaa gatagccctt
     1501 aaatataatc cgaaaagcaa acagcctgca attaagagcc tgcagagagt cagccgtccc
     1561 ggtctgaggc attatgctgc tgccgataag atgccgcgtg tgctcaacgg gcttggaatt
     1621 gcaattatct cgacttcaag gggactgatg accgacaagg aagcaaggaa agagaatatt
     1681 ggcggtgagg tattatgtta tgtatattaa tcaggaggga agcttatgtc aagaatagga
     1741 aaaatgcccg ttagcctgcc ggcaggagtc agtgtggaaa tcagcggcaa taatgtgatt
     1801 actgttaagg ggccgctggg tcagctgagc aagcccgttg acagcgatat tgaaattgag
     1861 gtttctgaca atgagataca ggtaaagagg ccgtccgagt cgaagcatca caaatcgctt
     1921 catggccttt acagggcact tattgccaat atggtaacag gtgtttctga gggattcaaa
     1981 aaggaacttg aactggtggg tgtcggctac cgtgccgaag ccagaggcca ggatcttgaa
     2041 ctgagccttg gttattccca tgatattatt ttcaggctgc ccgatgaggt aaaggtggaa
     2101 acgaaaactg aaagaaggag caatcccatt ataacactca catccgccga caagcagctt
     2161 atcggtcatg ttgctgcaaa aatcagatca cttcgcccgc ccgagccctt caaaggaaag
     2221 ggaataaagt tcgtaggcga ggaactgagg aggaaagccg gaaaatccgc cagtgtttaa
     2281 tatgttgata atcaggaaaa gttaataaga tggctttaag taagttggac agaagaaaaa
     2341 ggatcaggat gaggatccgg aaaaagataa acggtactgc agagatgcct agagtggcgg
     2401 tatttcgcag taacaagcag atatattgtc agttgattga tgatcagaat catgttaccc
     2461 ttctttctgc ctcctcgaag gagaaagaga ttgcttcgga aacaggaata aaaaagacag
     2521 agcaggcaaa actggtcgga aagcttctgg cagaaaaatg cagggacaag ggcattgaac
     2581 aagttgtatt cgacagaagc gggtataaat atcacggaag ggtcaaatcg cttgcagatg
     2641 cagcccggga aggcggacta aaattctaag ttattattat ggcagacagt ataagaaaag
     2701 taaaaaccag tgatcttgaa ctgaaggaca ggctggtaag cattcaaagg gttacaaaag
     2761 tcacaaaggg cgggcgtaca ttcagcttct cggccattgt ggttgtggga aatgaagcag
     2821 gcatagtagg ccatggcctc gggaaggccg gcgaggttac caccgcaata gcgaaaggca
     2881 ttgaggatgc aaagaagaac ctgatcaagg ttccggttat aaaaggaacc gtaccccatg
     2941 aacaggaagc caagtacggg ggggcaaggg ttttcattaa gcctgcatcg gaaggtaccg
     3001 gtgtcaaggc cggaggtgcc atgagggccg ttctggaaag tgtcggtatc aaggatgtgc
     3061 ttgccaagtc gaagggatct tcaaaccccc acaaccttgt gaaagctact attgcagccc
     3121 tgctggaaat gagggatgcc tattcggttg ccggtcacag gggcataccg gttcagagag
     3181 tgtttaacgg ctgatgataa attaattgaa atggcaaaaa tccgtattac ccaggttaaa
     3241 agcagaatcg gaaaacccga aaatcagaaa aggacacttg atgctctcgg tatccgtaaa
     3301 ctgaatcaca gtgttgaaca tgaggctaca ccccagattc tgggaatggt caataagata
     3361 aaacaccttg tcagggtgga aaatatatga tgttgaaaaa taaagataat aaagtgatgg
     3421 atttaagcaa tttgaaaccg gctgaaggat cggtcagaaa gggcaggcgc ctgggaagag
     3481 gccagggttc gggaagggga ggaacatcca ccaggggcca taagggtgcg aaatcgaggt
     3541 ccggatattc aaagagaatc ggctttgaag gcggacagat gccgttgcag agacgcgttc
     3601 cgaaatatgg ttttacaaat ataaatcgca gggaatacaa gggtattaac ataggaatgc
     3661 ttcagaccct ttcagaaaga ga
//
LOCUS       DAOSPX010000321         3653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_22816_length_3653_cov_4.937465, whole genome shotgun sequence.
ACCESSION   DAOSPX010000321 DAOSPX010000000
VERSION     DAOSPX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22816_length_3653_cov_4.937465"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLZ75_12465"
     CDS             <1..430
                     /locus_tag="PLZ75_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOS73249.1"
                     /translation="GFKGLLDARAVDIIHPDIGTSGGLLETKKIGEYAEENHVAMALH
                     MAGSPVCLMASVHCAAATQNFLAVEHHSVDVPYYNNLVRMTGSKPMIENGFANVPLDS
                     PGLGIELNEEECKKHLAKGSGWFEPIPEWDQKRSHDRLWS"
     gene            569..1840
                     /locus_tag="PLZ75_12470"
     CDS             569..1840
                     /locus_tag="PLZ75_12470"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOS73250.1"
                     /translation="MKKQLFILIILAFLSSYAGKSQIPVQAGPGQKLAELKNQLANDL
                     KNNILPYWSSKMPDEINGGFFGRIDASNRVIHDASKGGILNARILWTWSAAYRVTGNP
                     EYLRLATRARNYILSNFIDRKHGGAYMTLNHKGEIQDDRKHTYTQTFFIYGLSEYARA
                     TGDRKALKEAKNIFKALEKNALDREANGYFEVFSRDWQRLHDRLIGEKSPADEKSMNT
                     SLHIMEAYANLYRVWPTARMAERLRNMVNIFLDHIIDKNTHHLIYFLDRNWNSTSGID
                     SYGHDIEASWLIYEAATLLGDPELIRKAREASVAIADAAAEGLMPDGSMLSEKDRETG
                     RIRDTRGWWDQAEAVVGYVNAYELTENEDYLNMAVKTWNFIEKYLIDRINGGWFAYVS
                     PEGTPGGDKAGTWICPYHNSRMCLEIMERVK"
     gene            1877..2782
                     /gene="rbsK"
                     /locus_tag="PLZ75_12475"
     CDS             1877..2782
                     /gene="rbsK"
                     /locus_tag="PLZ75_12475"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOS73251.1"
                     /translation="MKHILVIGSSNTDMVVKTEKFPAPGETVLGNTFFMNPGGKGANQ
                     AVAASRLGGRVTFISKLGRDYLGDQYIKTLKKERIDTRHVIRDDKLPSGVALITINSA
                     GENNIVVAPGANFNLLAGEIDTNLFSSGKYSILLVQLEIPLVTVEHAVKTAAGNRMKV
                     ILNPAPARELPGHLMKQLWLITPDENEATELTGIRVSDELSAEQAASALTGKGVKNVI
                     ITMGSMGAYVKSEEFTGMIPAVKAEPVDTTAAGDVFNGALAVALTEGAGIKDAVIFAN
                     RAAALSVTRLGAQSSAPYRNELQHG"
     gene            2818..>3653
                     /locus_tag="PLZ75_12480"
     CDS             2818..>3653
                     /locus_tag="PLZ75_12480"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOS73252.1"
                     /translation="MKKSFSVLTVMAVYLLAASCTGTSGEYRNIRPDVLKDKIAGGWA
                     GKMAGVSYGAPTEFKAQGRTYEEEINWDPSDIRGSLWQDDIYVQLTFLMAMDKYGIDG
                     PAKKFQEMLATAGYPLWHANMQARKNFHDSIFAPLSGSPEYNIHADDIDFQIEADYIG
                     FMCPGMPRKALEIADKIGRIMNYGDGIYGGVFVAALYTEAFFENEISEIIEKALLSIP
                     SGSDYYRIIKDVIKLHGHYPDDWRAAWRELEDKWGDVDICGAGSPFNIDAKLNGAYIA
                     MG"
BASE COUNT         1088 a          823 c          936 g          806 t
ORIGIN      
        1 cggcttcaaa ggtctactgg atgcaagggc tgttgacatc atacatcctg acatcggcac
       61 ttcgggcggt ttgcttgaaa cgaagaagat aggggagtat gccgaagaaa atcacgtggc
      121 catggcgttg catatggcag gatcacccgt ttgtcttatg gcaagtgtgc attgtgcggc
      181 agcaacccag aatttcctgg cagttgaaca tcacagcgtg gatgtgccgt attacaacaa
      241 ccttgtcagg atgacgggtt caaaaccaat gatagaaaac ggcttcgcca atgtaccact
      301 tgactctccc ggtctgggaa tagaactaaa cgaggaggaa tgcaaaaaac atcttgcaaa
      361 aggatccggc tggtttgaac cgatacccga atgggaccag aaacgttcac atgacagatt
      421 gtggagctga ctccggagat tcaaatcagc cggtgacaat gattcagctc aagcttgttg
      481 gctgcggatg gataaaacat tgaataccag ctaccctgaa caggacaaga atgtaccatc
      541 ccggttataa ccaccagatt aacgagacat gaaaaaacag ctttttatcc tgataattct
      601 ggcattcctg agcagttatg ccggtaagag tcagataccg gtccaggccg gtccgggtca
      661 gaaactggcg gaactgaaga atcagcttgc caatgatctg aaaaacaata tccttcccta
      721 ctggtcatca aaaatgcccg acgagattaa cggaggcttt ttcggaagga ttgatgccag
      781 caacagggtt attcatgatg cttcaaaagg cggaattctg aatgcaagaa tattatggac
      841 ctggtcagct gcatacaggg tcacaggaaa ccctgaatac ctccggctgg cgacccgtgc
      901 cagaaattat atattgtcga attttatcga caggaagcat ggaggggcat atatgactct
      961 gaatcacaag ggtgaaattc aggatgacag gaaacacacc tatacccaga ccttctttat
     1021 atatggttta tcagaatatg ccagggccac tggtgacagg aaagcattga aagaagcaaa
     1081 aaatattttc aaagcgcttg agaaaaatgc cctcgacagg gaggcaaacg gctattttga
     1141 ggtgttcagc cgcgactggc agcgcctgca cgacaggctt atcggagaaa agagccctgc
     1201 cgatgaaaaa agcatgaaca cttctcttca tataatggaa gcctatgcaa atctttaccg
     1261 ggtgtggccc actgcacgca tggccgaaag actgaggaac atggtgaata ttttccttga
     1321 ccacataata gacaaaaaca ctcatcatct gatatatttt ctggacagga actggaacag
     1381 cacttccggt attgattcct acgggcacga tatagaggcc tcctggctga tctacgaagc
     1441 ggccacgctg ctcggggatc cggaactgat ccgaaaggca agggaagcaa gtgtggcaat
     1501 agctgatgca gctgctgaag gactgatgcc agatggcagc atgctgagcg aaaaagaccg
     1561 ggaaactggc aggataaggg atacccgggg ctggtgggac caggcagaag cagtggtggg
     1621 atatgtcaat gcctatgaac ttacagaaaa tgaggattac ctcaacatgg cagtaaaaac
     1681 ctggaacttt attgaaaaat atctgataga caggataaac ggaggatggt tcgcatacgt
     1741 ttcaccggag ggcacacccg gaggtgacaa ggccggaacc tggatatgcc cctaccataa
     1801 cagccgtatg tgcctggaaa taatggaaag agtgaaataa aacaaatcat cctctcatat
     1861 cataattttt gaaatcatga aacatatcct tgttatagga agctccaata ccgacatggt
     1921 agttaaaacc gaaaaattcc cggcccccgg tgaaaccgta cttggaaata ccttcttcat
     1981 gaatccgggg ggaaaggggg caaatcaggc tgtggcagct tcccggctcg ggggcagggt
     2041 cacgtttata tcaaaattag gcagagacta tcttggcgat cagtatatta aaaccctgaa
     2101 aaaagagagg atagacacca gacatgttat cagggatgac aagcttccct cgggagttgc
     2161 tcttatcacc atcaattctg cgggggaaaa caacattgtt gttgccccgg gcgccaattt
     2221 taatcttctt gccggagaaa ttgacactaa tctcttctca tcgggaaaat attccatcct
     2281 gcttgtacag cttgaaatac cacttgtgac ggttgagcat gccgtaaaga ctgcagccgg
     2341 aaacaggatg aaagtgatac ttaaccccgc tcctgcccgg gagttgcccg gccatctcat
     2401 gaaacagctc tggctcatta ctccggatga gaacgaagca actgaactga caggcataag
     2461 ggtaagcgat gagctgtcgg cagagcaggc tgcttctgca ttaacaggga aaggcgttaa
     2521 aaatgtgatt ataaccatgg gctcaatggg tgcatatgtt aaatcggaag agttcacggg
     2581 aatgattccc gcagtaaaag cagaaccggt cgacaccacg gctgcagggg atgttttcaa
     2641 cggtgctctt gcagtcgccc tgacagaagg tgcgggaata aaggatgccg ttatttttgc
     2701 caacagagct gcagcccttt ccgtaacccg actgggggca cagtcatccg caccgtacag
     2761 gaatgaattg cagcacggct gaacgctgaa gtgaaaaaca aaataaaact ctcaccaatg
     2821 aaaaaatcat tctccgttct cacagttatg gcagtttacc tcctggctgc ctcctgcacc
     2881 ggcacatccg gtgaataccg caatatcagg ccagatgtcc tgaaagacaa aatcgccggc
     2941 ggctgggccg ggaaaatggc aggcgtaagc tacggcgccc cgacagaatt caaggcccag
     3001 ggcaggactt atgaggaaga gataaactgg gacccttctg acatccgggg cagcctctgg
     3061 caggatgaca tttatgtaca gcttaccttc ctcatggcca tggataaata cggaattgac
     3121 ggcccggcaa aaaagttcca ggaaatgctg gccacggcgg gatatcccct ctggcatgcc
     3181 aatatgcagg ccaggaagaa tttccacgac agcattttcg cccctctttc aggaagtccg
     3241 gaatacaata ttcatgcaga cgatattgac tttcagattg aagcggatta tatcggtttc
     3301 atgtgccccg gaatgccccg caaagcacta gagatcgctg ataagatcgg ccggataatg
     3361 aattacggag acgggatata cggaggtgtt ttcgttgccg ctctatatac ggaagctttt
     3421 tttgaaaatg aaataagtga aataatagaa aaagcccttt tgtcaattcc gtcaggcagt
     3481 gattattaca ggattataaa ggatgtgata aaacttcacg gacactatcc cgatgactgg
     3541 agggcagcat ggagggaact tgaggacaag tggggggatg tggacatatg tggtgccggc
     3601 agcccgttca acatagatgc caaactcaat ggtgcctaca tagccatggg ctt
//
LOCUS       DAOSPX010000322         3652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_22826_length_3652_cov_3.593272, whole genome shotgun sequence.
ACCESSION   DAOSPX010000322 DAOSPX010000000
VERSION     DAOSPX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22826_length_3652_cov_3.593272"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /gene="pnuC"
                     /locus_tag="PLZ75_12485"
     CDS             <1..538
                     /gene="pnuC"
                     /locus_tag="PLZ75_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOS73253.1"
                     /translation="VAGIVYVFLEIRQNIWLWPVGIVTASVYLWVFFTGRLYAEMSLQ
                     GYYLIISIIGWYWWGMRTGGKQPQGKLKVSRIRSGPAVILAIILIVVYTVIWFVLDRF
                     TDSPVPEWDSFITSFSIIGTWMLARKIYEHWYLWIIANSAASVLFFSRGLYPTMLLYI
                     IYLVMSFAGLKAWKKSFK"
     gene            complement(531..1556)
                     /locus_tag="PLZ75_12490"
     CDS             complement(531..1556)
                     /locus_tag="PLZ75_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS73254.1"
                     /translation="MILSRRKFIENSAAVAALSMVPGSGLKGTYSKISPVSSSPDSKF
                     GGVQIGAITYSWRSMPGGIENIIKYCRQSGLSAIELMSGDLESYLGAPANPMAGMFGP
                     PPGGQRPAGTPGQRSQRTPEQEAAIAKYNKDLKEWRLTLPMSKVEQARKLFNDAGIKV
                     HIVKFSPARWSDEEIDYAFRTAKAMGAGGVTEEIGEEAAAKLGPIAQKHGMYAIFHNH
                     MQFATPGFSYDPFLAVSPAVMMNFDAGHFFGSTGIHPNTIIEKYHERIYSIHLKDKTG
                     PKTDPANTNQVWGQGEVPIADVLLLIKKQKWPIYIDIELEYDIKPWSDAVKETKTCLE
                     FARQILI"
     gene            1740..>3652
                     /locus_tag="PLZ75_12495"
     CDS             1740..>3652
                     /locus_tag="PLZ75_12495"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOS73255.1"
                     /translation="MEQAASASRSARSQIKDRFLFIRENTKLIARFLLAVLFIAVGAW
                     FLKHEQAETGEIKRILLTARLEYLGLGILVTLFYIVLQGFMYKMAFASIKSRVSLGLT
                     TMLFLKRNLISIFMPAGGIASLAFFSEDIQKKGESRTKILFASSIYAFVGILTIIMVG
                     IPVLLYTMFKGITGSGEVFALGAVILIVISLFVSFRSILKKKYLYKTIVKYLPSMEVF
                     LGDLISHKIDINYLIITVLVSVLIDMTGIIHLYIAMLALGFKASLFYAMLGYLTAVIS
                     LSLSPFIRGLGAVEISMSFILVRLGYTNIEAIAITFLYRFFEFWLPLLSGALSFFLKI
                     NKILFRIFPALLIFTLGLINIISSITPAISERIHILEDLIPVNAIVASNYFVFIAGAF
                     MLLTAVFMLRGSRNAWWIGLILSIVSCIGHLTKAIDYEEASIALIMVIILIFSRKEYN
                     LKSNPRLHTVGIWSSVLSMTVVLIYGTIGFYFLDEKHFGADFNLWQSIIYSIKNFILI
                     GSPDLVPGSHFAKYFLISMNINGLLTFSFLFYTIIRPYFHKVSPAPEELDNAKRLVEI
                     YGSSALDYFKTYRDKMIFFTEKRDSFISYRTAGNYAVVLENPVAANQAAMEQCIILFD
                     KFCYVNGLKNIFYR"
BASE COUNT          916 a          729 c          785 g         1222 t
ORIGIN      
        1 cgttgcaggt attgtctatg tttttctgga gataaggcag aacatctggc tctggccggt
       61 cgggattgtc accgcatcag tctatttgtg ggtttttttt accggcagac tgtatgctga
      121 aatgtctctt cagggttatt atctgattat cagtattatc ggatggtatt ggtggggaat
      181 gcggacagga ggaaagcagc ctcagggaaa gctgaaagta tcgagaatca gatccggtcc
      241 ggcagtaatt ctggccatta tcctgattgt ggtttatacc gttatctggt ttgttctcga
      301 caggtttacg gattccccgg ttccggaatg ggattccttt attacttcct tttcaataat
      361 cggcacatgg atgctggccc ggaagattta tgaacactgg tatctgtgga tcattgcaaa
      421 ttctgctgcc tctgtcctgt tcttctcaag agggctgtat ccgacaatgc tgctttatat
      481 tatctatctc gtgatgtcct ttgccggtct gaaggcatgg aaaaaaagtt tcaaataaga
      541 atctgccttg cgaactcaag gcatgtcttt gtttccttca cggcgtcaga ccatggcttg
      601 atgtcatatt cgagttcaat gtcgatgtat attggccatt tctgcttctt tatcagtaaa
      661 aggacatcag caatcggcac ttcaccctga ccccacacct ggttggtatt tgccggatca
      721 gttttcgggc cggttttgtc cttcaggtga atactgtaga tacgttcgtg gtatttttca
      781 attatggtat tggggtggat gcctgtggaa ccaaagaaat ggccggcatc aaaattcatc
      841 atcacagccg gggatactgc caggaaagga tcatagctga accccggtgt ggcaaactgc
      901 atatggttgt gaaatatggc atacattccg tgtttttgtg ctatcgggcc aagttttgcg
      961 gcagcttctt ccccgatctc ttcagtaaca cctccggctc ccattgcttt cgctgtcctg
     1021 aaggcatagt caatttcctc atccgaccag cgtgcggggg agaatttgac tatatggacc
     1081 tttattcccg cgtcattgaa taattttcgg gcctgttcca ccttggacat tggcagggta
     1141 aggcgccatt ccttcagatc cttgttgtat tttgcaattg cagcttcctg ttcaggggtc
     1201 ctttgagagc gttgccccgg ggttcctgcc ggacgctgac ctcccggggg aggaccaaac
     1261 attccggcca tgggatttgc cggggccccc aggtaagatt ccaggtcgcc gctcataagc
     1321 tctatggcac tgagccctga ttgcctgcaa tactttataa tgttctcaat tccgcccggc
     1381 atgcttcgcc agctgtatgt aatagctccg atctgtacac ctccgaattt cgaatcgggg
     1441 gaagatgaaa caggagatat tttggaataa gtacctttca gtcctgaacc gggtaccata
     1501 ctcaatgccg caacagcggc actgttttca atgaatttac gtcttgagag tatcatattt
     1561 acttttttag ttagttgcta aaacgtgtta ttcgacaaca catcaaaatt ccaactgcaa
     1621 acagattaat ctaccgggat taccggatat aaaaactttt cattaattgt aaatataaga
     1681 ataatcatat ttacatataa ttatatatcc tgtaaatttg tattacatgt tacctgttta
     1741 tggaacaagc tgcttctgca tcccggtcag cgcgatctca gataaaagac agatttctgt
     1801 ttatcaggga aaatacaaaa ctcattgcgc gatttttatt agctgtttta tttattgctg
     1861 tcggtgcatg gtttttaaaa catgaacagg cagaaacagg tgaaataaag cggattttat
     1921 taactgcacg attggaatac ctggggctgg gtatactggt gacattgttc tatatcgtac
     1981 ttcagggatt catgtataaa atggctttcg cttcaattaa gagccgggta tctctgggat
     2041 tgactaccat gctatttctc aaacgcaatc tgatcagcat tttcatgcct gccggtggta
     2101 tagcctctct tgcttttttt tctgaggata ttcaaaaaaa gggagaatcc aggactaaaa
     2161 tcctctttgc atcatctatt tatgcatttg tcggaatcct cacaattatc atggtaggaa
     2221 ttcctgtttt gctttataca atgttcaagg gtattaccgg gtccggtgaa gtttttgctt
     2281 taggagcagt gattctgata gttatttcac ttttcgtatc gttccgctca atattgaaaa
     2341 agaaatactt atacaagacc attgtaaaat accttccttc catggaagtc tttctcggag
     2401 acctgataag tcataaaata gatattaact atcttattat tactgtttta gtatccgttc
     2461 tgattgatat gacaggtatt attcatctgt atatcgctat gttggcctta ggtttcaagg
     2521 cttctctttt ttatgcaatg ctgggatatc tgactgcagt aatttctttg tctttatctc
     2581 cattcatcag gggacttggt gcggttgaga tatcaatgtc atttattctt gtccggttgg
     2641 gttatacaaa tattgaagct attgcaataa catttctgta caggttcttt gagttctggc
     2701 tgccattgtt atcgggagca ttgagtttct ttttgaagat aaacaagata ctgttcagaa
     2761 ttttccctgc attattaata ttcacactgg ggctcataaa cattatttct tccattacac
     2821 ctgctatttc cgaacggatt catatacttg aggacttaat tcccgttaat gccattgttg
     2881 cctcaaatta ttttgtcttt atagcaggtg catttatgct gctgacagct gtatttatgt
     2941 tgaggggttc gcgtaatgcc tggtggattg gtctgatcct tagcattgtt tcatgtatcg
     3001 gtcatctgac aaaagcaata gattatgagg aagcatcgat tgctttgatt atggttatca
     3061 tacttatttt ttcgagaaag gaatataatc ttaaaagtaa cccccggtta cataccgtgg
     3121 ggatctggtc atcagttctg agcatgactg tcgtactgat ttatggtaca atcggttttt
     3181 attttctcga tgaaaaacac ttcggggcag actttaattt atggcaaagt attatttatt
     3241 caattaaaaa cttcatcctg attggaagcc ctgatctggt acccggcagt cattttgcaa
     3301 aatattttct tatctcaatg aatattaacg gtctgttgac cttttctttt ttgttttata
     3361 caatcataag gccttatttc cataaggttt caccggctcc ggaggagttg gataatgcaa
     3421 agcggctggt tgagatttat ggcagctcag ccctggatta ttttaaaacc tatagggata
     3481 agatgatatt cttcacggaa aaacgcgact cttttatttc ttacaggacg gcgggtaatt
     3541 atgccgtcgt acttgagaat ccggttgccg cgaaccaggc agcaatggaa caatgtataa
     3601 tactcttcga taaattctgt tatgtaaacg gattgaaaaa tattttttac cg
//
LOCUS       DAOSPX010000323         3637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_22954_length_3637_cov_4.826075, whole genome shotgun sequence.
ACCESSION   DAOSPX010000323 DAOSPX010000000
VERSION     DAOSPX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22954_length_3637_cov_4.826075"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /gene="metX"
                     /locus_tag="PLZ75_12500"
     CDS             complement(<1..1058)
                     /gene="metX"
                     /locus_tag="PLZ75_12500"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOS73256.1"
                     /translation="MKTYRYTKPFRTETGSFLPGLEIAYHTYGKLNEKADNVIWVCHA
                     LTANSDIEAWWPGMVGKDLLFDTEKYFIVCANVLGSCYGTTGPASLNPDTGRPWLRDF
                     PLITIRDLVNVHEILRQHLGIKSIHTVIGASIGGFQALEYAIMQPGLIRRLIVLASGA
                     RQSPWALAFSESMRLAMEADDSFTANDPEGGKKGLKAARSIALISYRTAEAYNTTQQE
                     EDDEKLTSFRAASYQAYQGDKLVRRFNPYSYWTLTCMSDTHNIGRGRGGILNALKTIK
                     AEILCVGIGSDLLFPVTDQKYIAENASNAKYTEIDSFYGHDGFLIETEKVTEIVRKFW
                     ENSNKKQQNDKAETTDRA"
     gene            complement(1055..2359)
                     /locus_tag="PLZ75_12505"
     CDS             complement(1055..2359)
                     /locus_tag="PLZ75_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS73257.1"
                     /translation="MKNHNPKFETLQVHAGHQPDSDTLSRAVPIYQTTSYVFRNAKHA
                     ADLFSLEEAGNIYTRINNPTTEILENRIAALEGGVAALAVASGHAAQFTALSSILQKG
                     DNFVSSPFLYGGTYNQFKVTFARFGIECRFSKDLDPASFEKLIDGNTRAIYVETIGNP
                     GFDIPDFAAFSELAQKNGIPLIVDNTFGAGGYLCRPIDHGANIVTESATKWIGGHGNS
                     IGGVIIDAGNFNWLNGKFPAFSEPSEGYHGLVFGEKFSESSGTGNIAFIVRARVESLR
                     DLGSSISPFNSFLLLQGIETLSLRMERIVQNTQALAEWLHEQPLVEKVYYPGLPGDRN
                     YHLAQKYLPNGAGGVLSFVLGLEKGKAVKLVEHFRIVSHLANVGDTRTLIIQPSATTH
                     SQLSEEEQAAAGIDPGLFRVSVGIEHIDDIIDDFSHAFKQIK"
     gene            complement(2473..2730)
                     /locus_tag="PLZ75_12510"
     CDS             complement(2473..2730)
                     /locus_tag="PLZ75_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73258.1"
                     /translation="MVWQIITVPGPAVSEEVSDNRNRQPYRPEKKFYHELIMNGITAP
                     YKNSDRKPCGEYITLPEGSFCPFHLSSNNFTMPLMPMGMLG"
     gene            2960..>3637
                     /locus_tag="PLZ75_12515"
     CDS             2960..>3637
                     /locus_tag="PLZ75_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HOS73259.1"
                     /translation="MQKINDFLIMLDGYIGGHPWFVILLLGTGIFFTLYLKFPQFRYF
                     RHAIDVVRGKYDHHLDIGDTSHFQALSTALSGTVGTGNIAGVALAIHLGGPAALFWML
                     VTAAIGMCTKFVEVLISHKYRDILPDGTVSGGPMYYMKKRLNITTRSGRVIKTGAVLG
                     TFFAAATVLSSFGTGSLPQINSISNSIFTTFGIKHIITGAVLSGVMALIIIGGIKRIA
                     QVTEKLVP"
BASE COUNT          913 a          839 c          828 g         1057 t
ORIGIN      
        1 atagcccgat ccgtagtttc tgccttgtca ttctgctgtt ttttatttga gttttcccag
       61 aatttcctga caatttctgt aaccttttct gtttcaatca ggaaaccatc gtgtccgtaa
      121 aatgaatcta tttcagtata ttttgcattt gaagcatttt cagctatata tttctgatca
      181 gtaaccggaa acagtagatc cgatcctatg ccgacacaca gtatttcagc ctttattgtt
      241 ttcagggcat tcagtattcc cccccttcct ctgccaatgt tatgggtgtc ggacatgcat
      301 gtaagtgtcc agtaggagta aggattgaac ctcctgacaa gcttgtcgcc ctggtaagcc
      361 tggtaggatg ctgccctgaa tgaagtcagt ttttcatcgt cctcttcctg ctgggttgta
      421 ttataagcct ctgctgtcct gtagcttata agcgcaatcg atctggcggc cttcagccct
      481 tttttgccac cttccggatc atttgcagtg aaggaatcat cggcctccat ggccagtctc
      541 attgattcac tgaaggcaag ggcccacgga gactgtctgg caccggatgc aagaactatc
      601 agcctccgga tcagtccggg ctgcatgatg gcatattcca gggcctggaa tcctcctatt
      661 gatgctccga taactgtatg aatgcttttt atcccgagat gctgcctcag tatctcatgc
      721 acattcacaa gatcccttat tgtaatcagt ggaaaatccc tgagccaggg cctgccggta
      781 tcggggttca gcgatgcggg tccggtagtc ccgtaacagg aacccagcac gttggcacag
      841 acaataaaat acttttccgt gtcaaacagg aggtccttgc ccaccattcc tggccaccag
      901 gcttcaatat cggaatttgc agtaagggca tggcagaccc agattacgtt atcagccttc
      961 tcattcagtt tgccgtaggt atgataggcg atttccagtc cgggaaggaa gctccctgtt
     1021 tctgtcctga aaggttttgt atatctgtag gttttcattt tatctgttta aaggcgtggc
     1081 tgaaatcatc aatgatatcg tctatatgct cgatgcccac ggataccctg aaaaggccgg
     1141 gatcaatccc tgcagcggcc tgttcctctt ctgaaagctg ggaatgcgtt gtagcggaag
     1201 gctgtataat gagggtcctg gtatcaccca catttgccag atggcttaca atacggaaat
     1261 gttcaacaag ttttaccgct tttccctttt caagtccgag gacgaagctc aggacaccgc
     1321 ccgccccgtt gggaagatat ttctgtgcaa ggtgatagtt cctgtcaccc ggaagcccgg
     1381 gataatatac tttttccaca agaggctgtt catggagcca ttcggcaagc gcctgtgtat
     1441 tctgcacaat tctttccatg cgaagcgaaa gcgtttctat tccctgaagc agtaagaagg
     1501 aattgaacgg gcttatggaa gagccgaggt cacgcaggct ttcaaccctt gccctgacaa
     1561 tgaatgcaat atttcctgtt cctgatgact ctgaaaattt ctcaccaaaa acaagtccgt
     1621 gataaccctc agaaggttca gaaaatgccg ggaatttgcc attcagccag ttgaaattgc
     1681 cggcgtcaat tatcacgcct cctatgctgt ttccgtgacc gccaatccat tttgttgcag
     1741 attcggttac aatattggca ccatgatcga tcggtctgca gagataaccc ccggccccga
     1801 aagtgttatc cactatcaga ggtatcccgt tcttctgtgc aagttcggag aatgccgcga
     1861 aatcaggtat gtcaaatccc ggattcccga ttgtctcaac atagatggcc ctggtattgc
     1921 cgtcaatcag tttttcaaat gaggcggggt caagatcttt cgaaaaacgg cattcgatgc
     1981 cgaatctggc aaatgtaacc ttgaactggt tataggtacc gccgtaaagg aagggagaac
     2041 tgacaaagtt atcacccttt tgcaaaatcg aggaaagagc agtgaactgt gcagcatgcc
     2101 cggatgcgac agccagtgcc gcaacacctc cctcaagggc agctattctg ttttccagta
     2161 tttcagtagt ggggttgttt atcctggtat aaatgtttcc ggcttcctca aggctgaaca
     2221 ggtcagcagc atgcttggca ttcctgaaaa catatgacgt ggtctgatat ataggtacag
     2281 ccctggaaag agtatcactg tcgggctggt gtccggcatg tacctgaaga gtttcgaatt
     2341 ttggattatg gtttttcatt ttctgatgaa ttacatgatt ttaaatattc cttgaaataa
     2401 aaatgagaat cggacaatca gggtttaaca ttgtccggcc gggttttcag aattccgctg
     2461 taaaacggaa atctagccga gcatgcccat aggcatcagc ggcatggtga agttgttgct
     2521 ggaaagatga aaagggcaga agcttccttc gggtagagta atgtattccc cgcagggttt
     2581 tctgtctgaa tttttatatg gtgctgttat gccattcatg attaattcat ggtaaaactt
     2641 cttttccggc ctgtacggct gcctgtttct gttatcagat acttcttccg atactgccgg
     2701 ccctggtaca gtaataattt gccataccat acccggttga aagcataaac aatgaccaat
     2761 catcttttgt tcgcgacttt aaaaaaaaat taccggattc tgattcccct ggaagtggca
     2821 agagcggacc atcaaaggga taaatgtttc attttccggc aaaacctttt aaggtcaatg
     2881 aatctgtaat tgttttttgt ccttttgtga aaattgttac ttttaccacc gcagtttttg
     2941 tcattctgaa acttaaccta tgcaaaagat caacgatttt cttataatgc tggatggata
     3001 cataggggga catccatggt ttgtgatatt attgctcgga accggaatat ttttcaccct
     3061 ctatcttaaa tttccccagt tcagatattt caggcatgcc attgatgttg tgcgggggaa
     3121 atatgaccac catcttgata taggagacac ctctcatttt caggcattgt caacggcact
     3181 ttccggtact gtgggtaccg gaaacattgc aggcgttgcc ctggcaattc atctcggagg
     3241 acccgctgca ctgttctgga tgctggtcac agcggccatc ggcatgtgca caaaatttgt
     3301 ggaagtactt atctctcata aatacaggga tatattgcct gacgggacag tatcgggcgg
     3361 tcccatgtat tatatgaaga aaagactgaa tatcaccacc aggagcggca gggttatcaa
     3421 aacaggagct gttctgggta cattttttgc cgcagccact gttctttctt catttggtac
     3481 aggaagtctt ccccagataa atagcatttc gaattcaata tttacaacat tcggtataaa
     3541 gcacattatt accggcgctg tcctttctgg tgtaatggcc ctgataatca taggaggaat
     3601 aaaacgtata gcccaggtca ccgagaaact tgtgccg
//
LOCUS       DAOSPX010000324         3620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_23095_length_3620_cov_3.763534, whole genome shotgun sequence.
ACCESSION   DAOSPX010000324 DAOSPX010000000
VERSION     DAOSPX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23095_length_3620_cov_3.763534"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..>3620
                     /locus_tag="PLZ75_12520"
     CDS             361..>3620
                     /locus_tag="PLZ75_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOS73260.1"
                     /translation="MRIRIVQLIFIVLLIPDLSAGIGTSSHSISRREGLSNGAVNAIV
                     RDAEGYIWLGTWNGLNRYDGHGIETFLPGSGPGSIHNHVIREIYPSTSGPLWMLTNRG
                     ISLYDNETGWFSAFFSDEPEQINYEMDVSLAFADDYGAVASVSGRGLFLFNEAAKQFV
                     PLPLDTVSHSDAMQIKRVHLDGNTLYCITSEGELFRLEGSRLAKILQLPLVSTLTTSS
                     IQAADGRPMLFITQRSGPALITDLESLEVQSLRLPDDVITSLFSSRLPDIIWVGTEKG
                     RIFRYSVINHTFTEPGKLNGQNSSLPFDTRILCIYETESDILWTGTDGNGAFTRKLAE
                     FPVTSLASEHFSYPIVRSILVTRKKEILIGTKGGGIDVFDDAGRRIRQISVKDGLSNN
                     SVLSFHERPDGSVWVGTDGNGIDLLSADYRTIRNFPGDICEKAPLTFASVYRIMEDSN
                     GDVYLGTSGYGVIQIKTGANGDPESCRQLMLDSGLASGLQKQIVYALTEERPGIIWIG
                     TRGFGVYRYNTTSQRVIAQYNSQTDPGLIRNDDILSLFTDCRGIIWVGSSSGLFSLEP
                     VSSDSVNPAGIDVQGGLPNFSIHTISQDFAGSLWITTNQGLSMIDSTRRNVQSFNMND
                     GLINFEYSDGASFFDKNSSRLLAGGTMGIDIVQTDSIHFSSYFPPIAINRLLIRNVPV
                     SPDEGSVLYKRINLQNELDLKYSQNSLSFNVSPLAYWGRERHRISYRLLNYSDEWVIL
                     LPDQPLAFSGLKPGSYTLQLRVSDENGIWSDIVREIAITVHPPFWLTGWAISGYIFLF
                     LLVQFLIFNAYRRREARRKEAALLEMKINQEKELQAYKIEFFTNVAHEFRTPLTLISS
                     HIHALIEDKKLTSENPRLLKVYNNSLKLQKLILEIMQFRKLEKGKEPLNIQETDPCSL
                     IREVVSDLEQLAAQRNIHCTVEGCETPFPFRTDADKFQRIITNLVSNAIKYNVEEGRV
                     VVKVAKADSLLTVEVHDTGIGINPEQLPRLFEPFGISSAKKRGNYPDYRSTGLGLVVT
                     KGLTELLKGSIQVKNRPEGGTIFICTFPEVFQVSADAPLVETDGSDSGRWILEEAE"
BASE COUNT          929 a          844 c          876 g          971 t
ORIGIN      
        1 atgctttgcc ggttcgatcc cggctccggg taccccggaa gcctcttgac taatttagtg
       61 ttgagaggct ttgtcatttg tgtgatacaa aagacacctt ttgctcctgc tctgatacct
      121 cgtttttctt cacgtctgta aaaagtctat agcagaggca ttccgttatt tccggggact
      181 cttcttccac tctacaatct gtttactaca gagaatgatt ctctgatggt aattgtaaaa
      241 aaatgcattc ttcaccagac cctaaattgg ctttccgctg gcgtgcttct ttcacagtat
      301 gtattgtttg gtattttgta ctaattttac ttctattcgg catgtgtaat tacttttctt
      361 atgagaatcc ggatcgtaca actgatattc atcgttttac tgattccaga tctgtctgcc
      421 ggaattggca catcgtcaca ctccatctcg cggcgcgaag gtttgagtaa cggagctgtc
      481 aatgccattg ttcgggatgc tgaaggatac atctggctgg gtacctggaa cggactgaac
      541 cggtatgacg gtcatggaat tgaaaccttt cttcccggaa gtgggccggg cagcattcac
      601 aaccatgtta tcagggagat ttatccctca acctcgggcc cgctgtggat gctgacaaac
      661 aggggtattt ccttgtatga taacgaaacc ggatggtttt ctgctttttt ttctgacgag
      721 ccggagcaaa tcaattatga aatggatgtc tccctggctt ttgctgacga ctatggagct
      781 gtagcatctg tatcaggccg gggcctgttc ctgttcaatg aggctgctaa gcagtttgtt
      841 cctcttcctt tggatacagt gtcacattca gatgcgatgc agataaaacg tgtacatctt
      901 gacggcaaca ctttgtactg tatcacttca gagggggagc tattccggct tgaaggcagc
      961 agactggcaa agattctgca actgcccctt gtcagcacac tgaccacttc atccatacag
     1021 gctgctgatg gcagaccaat gcttttcatt actcagcgtt caggccctgc acttattacg
     1081 gatctggaaa gcctcgaagt acaatctctg agacttcctg atgatgtaat aaccagtctg
     1141 ttttcttctc gtctgccgga cataatttgg gtcggaaccg aaaaaggaag gattttcagg
     1201 tattcagtca taaaccacac ctttactgaa cctggaaaac tcaacggaca aaacagcagc
     1261 ctcccctttg acacacgtat actctgtatt tatgaaacag agtcggatat tctgtggacc
     1321 ggaaccgatg ggaacggcgc atttaccagg aaacttgccg aatttccggt taccagcctg
     1381 gcttcggagc atttttccta tcccatagta agaagtatac ttgttacccg caaaaaagag
     1441 atactgatcg gtaccaaggg tggcggtatt gatgtttttg atgatgctgg cagacgtatc
     1501 cggcagatct cggtaaaaga cgggctgagc aacaactctg tgctctcatt tcatgaacga
     1561 cccgatggtt cagtatgggt gggcacggat ggaaatggta ttgatctgtt atcagccgat
     1621 taccgtacca tccgcaactt cccgggtgac atttgcgaaa aagcaccatt gacatttgct
     1681 tcggtttacc ggatcatgga agacagcaat ggagatgttt atcttggaac cagcggatat
     1741 ggcgtaattc agataaagac gggagcaaac ggtgatccgg agagctgcag acagcttatg
     1801 ctggactccg gtctggcatc cggattgcag aaacagatag tatatgccct gaccgaagag
     1861 agaccgggca ttatctggat tggcacccgc gggtttggcg tgtaccgcta caacaccact
     1921 tcgcaacggg tgattgcaca atacaattca caaacagacc ccggactgat ccgtaatgat
     1981 gatatcctct ccctctttac cgattgccgg ggtatcatct gggtgggcag cagcagcgga
     2041 ttgtttagcc tggaaccggt atcgtctgac tcggtaaatc cggctggtat tgatgtacag
     2101 ggcggattgc caaatttcag catccatacc atcagtcagg attttgcagg cagcctctgg
     2161 attacgacta accagggttt gtcaatgatt gacagcaccc gtcgcaatgt acagagcttc
     2221 aatatgaacg acggattgat caattttgaa tacagtgacg gagcttcatt ttttgataaa
     2281 aacagcagtc gtttactggc agggggaacc atgggtattg atattgttca aaccgattcc
     2341 atacatttct cctcatattt tcctccaata gcaataaacc ggttgctgat acgaaatgta
     2401 cctgtaagtc cggatgaagg aagcgttttg tataaaagaa tcaacctgca gaatgagctt
     2461 gatctgaagt acagtcagaa ttcactctcc tttaatgttt ctccgctggc ctactggggt
     2521 cgagagcggc accgtatctc ataccggctg ctgaactact cagatgaatg ggtaatactg
     2581 cttccggatc agccgctggc attttccggt ctcaaaccgg gaagttacac gcttcagttg
     2641 cgtgtgagcg atgagaacgg gatatggtct gacattgtca gggagatcgc tattacggtg
     2701 caccctccgt tttggctgac cggctgggca atctccggtt atatttttct tttccttctg
     2761 gtacagttcc ttattttcaa cgcctaccgc cgccgcgaag cacgccgcaa agaggctgct
     2821 ctccttgaga tgaagatcaa ccaggaaaaa gaacttcagg catataaaat cgagtttttt
     2881 accaatgtgg cacatgagtt ccgcacgcca cttaccctta tttcatcgca tatccatgcc
     2941 ctgatagaag ataaaaagct tacctctgaa aatccgcggc ttctaaaagt gtataacaac
     3001 agcctgaagc tgcagaagct gatacttgag atcatgcagt tccgcaaact ggagaaaggc
     3061 aaagaacccc tcaatattca ggaaacagat ccctgcagtc tgatcaggga ggtggtttca
     3121 gacctcgagc aactggcagc acagcgcaat atccactgta cggtggaggg gtgtgagact
     3181 ccattcccct tcaggactga tgccgacaaa ttccagcgaa tcattaccaa cctggtttca
     3241 aatgccatta aatacaatgt ggaagaaggt agggtcgtcg tgaaagtggc aaaggctgac
     3301 agcctgttga cagtagaggt tcacgatacc ggaatcggga taaatcctga acagctgccc
     3361 aggctttttg aacctttcgg aatctcttcg gcaaagaaga gaggaaatta tccggattat
     3421 cgctccaccg gacttggcct ggtggtaaca aaagggctga cggaactgtt gaaaggcagt
     3481 atccaggtca aaaaccggcc ggaaggtggc actattttca tctgcacttt ccctgaggtg
     3541 tttcaggtaa gtgctgacgc tcctcttgtc gaaactgacg gcagtgattc cggcagatgg
     3601 attctggaag aagcagagag
//
LOCUS       DAOSPX010000325         3577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_23458_length_3577_cov_4.544861, whole genome shotgun sequence.
ACCESSION   DAOSPX010000325 DAOSPX010000000
VERSION     DAOSPX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23458_length_3577_cov_4.544861"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1634
                     /locus_tag="PLZ75_12525"
     CDS             60..1634
                     /locus_tag="PLZ75_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOS73261.1"
                     /translation="MKNKLLLLNLCLLAALLITDASAQQTKPRIGIAGIQIENSVFMP
                     NRQAIIGRTASLPSYLSKDSVMGQSAIWLPSLIGRGGGRGPVTRESFETFVNNALEII
                     RSNMPYDAFWFYNHGACSVDGIDDPEGEFMERVRGVIGNDALVTTTMDLHGNVSWRVA
                     LYSDLITTYRTAPHDDAFESHRRGVVNLLERLSSGKGRPAYKAWVAVPVLLSGEWTST
                     RVEPAKSLYAMVPEVESLPGVVDAGIWIGYVWGDERRNQGVVMVVGDDKAQVENGAKK
                     LARRFWDVRRQFSLEAPGYPLEKCIDLAVASNKKPFLISDMGDNPGGGGSGEVTWTLA
                     RVLKRPEFKSPNGKKLLYCSIPGNEMVEAARKAGIGGQAEALVGAMTDNSYEGPVRLS
                     GTVIYVSPDHEKDQSSESKSPPDQRLPDIAIIKTGSIFVVVGTSSPTPNLEGTGIDPR
                     EMDIIMVKQGYLVSRWYDMQADWVMALTRGGVDQDFRSLPYKRIVRPIFPLDPDMPDP
                     ELNVIMVPSAKHMYGR"
     gene            1891..2697
                     /locus_tag="PLZ75_12530"
     CDS             1891..2697
                     /locus_tag="PLZ75_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73262.1"
                     /translation="MKNTLITVVALLIFISTWSCEKGEYTLPADFRLGMSISDKQAMG
                     GALTIDLIKIKLNAIDIQGYRETGGNVFLTRSFKKGQTFTIRANAKNEILNFDIPQGV
                     YKPLYFSLVFQHDDDEDDLIEDIEEWYEDVLEGDDPEELEEDLGSIIEDYLDDIEPSI
                     LVQGRFKSNKINVRLILLVNDPMTFRILLKNRSGQEEVILEKDRENTAELILDPSYWF
                     SVITPELMNRAYLGREDGITYLFLSKYVNSNIYHAVFNRMEESTYIIVNK"
     gene            2714..3271
                     /locus_tag="PLZ75_12535"
     CDS             2714..3271
                     /locus_tag="PLZ75_12535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOS73263.1"
                     /translation="MPGVSQKEDHILLEGCLNNDRGIQKKLFEKYFAKMFRICLSYSG
                     DRAEAKDILQEAFIKVFTGLDKFDSRGSLEGWIRRIVTNTAIDYFRQRKRLVYTATFP
                     DEPEDDGNSGFLFQELNQDIILDYVRQLPDGARVIFNLFALDGLSHREIGKKLNISEG
                     TSKSQYKRARGLLQKWLCEHEQDRQ"
     gene            3268..>3577
                     /locus_tag="PLZ75_12540"
     CDS             3268..>3577
                     /locus_tag="PLZ75_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73264.1"
                     /translation="MSNNAFDREYREWLQKLEVNKADSVWGGIEDQLDLIETWDRINT
                     GLDRVQPQRRKIIPLPFLRIMEVAAAILILVMIPLFFLKHQNNTISTVAEQRIPETDS
                     P"
BASE COUNT         1056 a          721 c          901 g          899 t
ORIGIN      
        1 aaaggtttat attcgttctt accataaatg aaattacata aaacaaacag gtcaaaaata
       61 tgaagaacaa gttattgcta ctaaatttat gcctgctggc agcgttgtta attaccgatg
      121 cctcagcaca gcaaacgaaa ccacgcatag gaattgccgg catacagatt gaaaacagcg
      181 tttttatgcc caaccggcaa gctataatag ggaggacggc ttctttgcct tcttatctta
      241 gtaaggattc cgtaatgggt cagtctgcaa tctggcttcc ttctttaatt ggcagaggcg
      301 gcggacgagg gccggttacc agagaatcat ttgaaacttt tgttaataac gcgcttgaga
      361 ttatccggag taatatgcct tacgatgcat tctggtttta caatcatggg gcttgtagtg
      421 tcgatggtat tgatgatccg gagggggaat ttatggagag ggtaagaggt gtaataggta
      481 acgatgccct tgttacaaca actatggacc tgcacggaaa cgtctcctgg cgtgtggctc
      541 tctactctga ccttattacc acatatcgta cagctcctca tgatgatgcg tttgaatcac
      601 atcgccgtgg cgtggttaat ctgcttgagc ggttatcgtc cgggaaggga cggccggctt
      661 ataaagcatg ggtggctgtc cctgtacttc tctccggaga gtggacaagt acccgggttg
      721 aacctgccaa atcactttac gcaatggtgc ctgaagttga gtccttgccc ggtgtggtgg
      781 atgccggtat ctggattggc tatgtttggg gagacgagag acgtaatcag ggtgtggtta
      841 tggtagtagg tgatgataaa gcccaggtgg aaaacggtgc aaaaaagctt gcccggagat
      901 tctgggatgt gcgccggcaa ttcagcctgg aagcaccggg gtatcccctc gaaaaatgca
      961 ttgatcttgc tgttgcaagt aataaaaaac ctttcctgat cagcgatatg ggcgacaatc
     1021 ctggtggcgg cggttcaggc gaggtgacct ggactctggc aagggtattg aaacggccgg
     1081 aatttaagag cccaaacggg aaaaaactgc tttattgctc cattccggga aacgaaatgg
     1141 tggaagccgc ccgaaaagcc ggtattggtg gtcaggcaga agctttggta ggggcaatga
     1201 ctgataattc atatgaaggt cctgttcgtt tatccggtac agtaatatac gtcagtcctg
     1261 atcatgagaa ggaccagtcg tcagaaagta aatcacctcc ggaccagagg cttcctgaca
     1321 tagcaattat taaaacggga agcatatttg tggttgtggg aacatcttca cccacaccaa
     1381 acctggaagg tacaggaatt gatcccaggg aaatggatat cataatggta aagcagggtt
     1441 atcttgtgag ccggtggtat gatatgcagg ccgactgggt tatggctctg acacggggag
     1501 gtgttgatca ggatttcagg agcctccctt ataagcgcat tgtcagacct atctttccat
     1561 tggatcctga catgccggat ccggaactga atgttataat ggtaccatca gcaaaacata
     1621 tgtacggcag ataagaggta aatatacctg gtcccggagc cccctggagc ggaaaccggg
     1681 agcattcaga caattgctcc cgccctgttt tttccgcatc ccgcaaacag caaggtgttc
     1741 ctgcctgttc tggcaaaata agccggacag gaatgtcaga cagataacag ggaaaaggaa
     1801 acagacaaaa aatcagttat cattacaacc tttcgtgtga actctcgtct tatccgtgat
     1861 attagtatca aaaagcatgt gcaacagaaa atgaaaaata cattaatcac agtagtcgca
     1921 ttgcttatct ttataagcac atggagctgt gagaagggag aatataccct tcctgctgat
     1981 ttcaggcttg gaatgtcaat cagcgataag caggccatgg gcggcgccct caccattgac
     2041 ctgataaaaa taaaactgaa cgcaatagac atacagggtt acagggaaac aggcggcaat
     2101 gtattcctga caaggtcttt taaaaagggg cagacattta ccataagagc aaatgcaaaa
     2161 aatgaaatac tgaactttga tattccgcag ggtgtttaca agcctttgta cttctctctg
     2221 gtttttcagc atgacgacga tgaggatgac cttattgaag atattgaaga atggtatgag
     2281 gatgttctgg aaggagatga tccggaggaa ctggaggaag atctcggatc aattattgaa
     2341 gattatcttg atgatatcga gccatccatt ttggttcagg gaagatttaa aagcaataaa
     2401 atcaatgtcc ggcttatact tctggtgaat gatcccatga ctttccgcat cctgttaaag
     2461 aacaggagcg gccaggagga agtaatactt gaaaaggaca gggaaaatac agccgaactt
     2521 atcctcgatc cttcctactg gttctcggta attactccgg agcttatgaa cagggcttat
     2581 ctcggcaggg aggatggtat cacatatctt ttcctgagca agtatgtcaa cagtaatatc
     2641 tatcatgctg tgttcaacag aatggaagag tcgacttaca taatagtaaa caagtagcga
     2701 ataattatcc ttcatgcctg gagtttcaca gaaagaagac catatactgc tggaaggctg
     2761 cctgaataat gacagaggga ttcagaaaaa gctgttcgaa aaatattttg caaaaatgtt
     2821 ccggatatgc ctttcataca gcggcgacag ggctgaagca aaggatatat tgcaggaagc
     2881 ttttataaag gtgtttacag gtctggacaa atttgattcc cggggttccc tggaaggctg
     2941 gatacgaaga atagtaacca atactgccat tgattacttc aggcagcgta agagactcgt
     3001 ttataccgct acctttccgg acgagccgga agatgatgga aatagcggat ttttgtttca
     3061 ggaactgaac caggatataa tcctggatta cgtcagacaa cttcctgacg gagcaagggt
     3121 tatttttaac ctgtttgccc ttgacggatt gtcacacagg gaaataggca aaaaactcaa
     3181 tatttcggaa ggaacctcca agtcgcaata caaaagggca aggggactcc tccaaaaatg
     3241 gctttgcgaa cacgaacagg acaggcaatg agcaataatg catttgacag ggagtacagg
     3301 gaatggcttc agaagcttga ggtaaataag gcggattctg tctggggagg aatagaagac
     3361 cagcttgacc ttatcgaaac atgggacagg atcaataccg ggctggacag ggtacagcct
     3421 cagaggagga aaattattcc actgcccttt ctccggataa tggaagtcgc agctgcaata
     3481 ctgattcttg tcatgattcc gctttttttc ctgaaacatc aaaacaatac cataagcact
     3541 gttgcggaac aaagaatccc tgaaacagat tcccccg
//
LOCUS       DAOSPX010000326         3567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_23558_length_3567_cov_4.370159, whole genome shotgun sequence.
ACCESSION   DAOSPX010000326 DAOSPX010000000
VERSION     DAOSPX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23558_length_3567_cov_4.370159"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2081
                     /locus_tag="PLZ75_12545"
     CDS             <1..2081
                     /locus_tag="PLZ75_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73265.1"
                     /translation="LRINGKTPKAKMWRWQPYQGLRKGDIDGFDILSSTRMLFDRDAV
                     MWQVDISNSDTVSRDLSIELDLIGFISKYGGDWQWWYPYPKMTGEVTKRDEEVENIRK
                     HLGEQVIAGEGMGWELVDGKPQLVKIMMRWPGDREILSSSKYSTTAGNNGVLIRDTET
                     DAIVSFRLLSPPDKMETFNSGAVVSWNRTAAPGETIRIKYLMSYGDDEDIIRKNLDTW
                     SASFDDYFSSIKKSWEEKWEQIFQPGNDLVSGCFPVLETKDPLVKKVYYTSPLTMLYL
                     TNTNLPQHDKVFLTGGPRWGASITFFWDITLWSVLWATVDPVMMKEHVSSWIRIDPSK
                     YYGKDNFGGRGVGNGYSANYWALFQMIRDYINTTGDFDFLDESIEGKTVFEHLENYAL
                     NWQKISVYGTEGATDDIYKLADFGDDEWNLLECVPTYKHIVPSFNVGYAWMMRETAKF
                     CRHRGDDRKADSLESLASEMISRILRLYAGNGVWNSIYPGNRTIEVRHCLDFAFFGKY
                     IPHDVPEKIRKEMITFLYDELITDHWMRAQSLFDVAAENSDRADHGPFGAFDGWPVDI
                     MDALTGMGFPDKALEFYRAVEPVTNEGIWAQAHELWGENKRNKNALVRITERDWCNRE
                     SSSGTAFAQVVLKDFLGFYPEADGEVLKSRGDFQFDGKLHHVLYRNNYYTLEYESEKA
                     VMKKEKIRKK"
     gene            2115..3512
                     /locus_tag="PLZ75_12550"
     CDS             2115..3512
                     /locus_tag="PLZ75_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73266.1"
                     /translation="MNKNKNISAFYTIMLLPGLLLFLTAPLTAQNVIRKAEDHFDRFT
                     NVAGGWCAGDATISLPLPDGKTIWLFGDSFIGRKSGKYSIISSGSRMINNAVILDDGT
                     EMTTIHGGSGDSPSSFIPPAGADIFWPEHAVMENDILKVFGVRIKFVDNQTPGFNFEV
                     GTTYKAAFTYPGFGHISTTKFTSVTDSTFRFGACVVKSGEYTYIFGVKDTTAGIFTYP
                     LPYLARVNESIDEPWQFYAGSGNWSYNCSEAVPVGDRPMSESFYVYEKNGRFYLIMHE
                     IWLKGELFILEADRITGPWNRRSSGGTENRFAVISPHAGNITYNLFAHPQFQDEGDIL
                     ISFNVNTSNFISIYQDTRNYRARFYWLDVEAAAVTVIPDTIRLFDDPVGIIDLHNGRP
                     ESRLVYDPLNGCLKLRDISSPALLEISGIDGRIYMEREIRDDAEINCRHLRGRMMIVS
                     LRGKWGAETRKFFFR"
BASE COUNT         1005 a          753 c          928 g          881 t
ORIGIN      
        1 cactcaggat caacgggaaa accccgaagg caaaaatgtg gcggtggcag ccgtaccagg
       61 ggctcaggaa gggggatatt gacggatttg acatacttag ttcaacaagg atgctctttg
      121 acagggatgc cgttatgtgg caggttgata taagcaacag tgacacggta agccgtgatc
      181 tcagcataga gcttgaccta atcgggttca tcagcaaata cgggggtgac tggcaatggt
      241 ggtatcccta tccgaaaatg acaggagagg tgacaaagcg cgacgaagag gtggagaata
      301 tcagaaagca tctgggagaa caggtaattg caggagaagg aatgggatgg gaacttgtcg
      361 acgggaaacc gcagcttgta aagattatga tgagatggcc gggtgacagg gaaattctca
      421 gcagctcaaa atacagtaca acagccggaa ataacggggt gctgatcagg gataccgaga
      481 ctgatgcaat tgtttctttc cggctgctca gcccgccgga taagatggaa acattcaaca
      541 gcggggctgt ggtttcctgg aaccgcacgg cggcaccggg agaaaccatc aggattaaat
      601 acctgatgag ctacggagac gatgaagata ttatccggaa aaaccttgac acttggtcag
      661 cctcttttga tgattatttc agcagtatca aaaagagctg ggaagaaaaa tgggaacaga
      721 tctttcagcc gggcaatgac cttgtttcag gatgcttccc cgtactggaa acaaaagatc
      781 cccttgtgaa aaaggtttat tacaccagtc cccttaccat gctgtatctg acaaacacca
      841 atctgcctca gcatgacaag gtatttctga caggtggacc tcggtggggg gcatccataa
      901 catttttctg ggacattacg ctgtggtccg tcctctgggc aaccgttgat ccggtaatga
      961 tgaaagaaca tgtttcatcc tggatcagga tagatcccag caagtattac ggaaaggata
     1021 atttcggggg caggggagtc ggtaatggct attccgccaa ttactgggct ctctttcaga
     1081 tgatacgcga ttatataaac accacgggtg attttgattt cctcgatgaa tccattgaag
     1141 ggaaaacagt attcgaacat ctggaaaatt acgctcttaa ctggcagaaa atatctgttt
     1201 acggaacaga aggtgctact gatgatatct ataagctggc tgatttcggg gatgatgaat
     1261 ggaacctgct tgagtgtgtg ccgacctata aacacatagt gccatccttt aatgtcggct
     1321 atgcatggat gatgcgcgaa acggcaaagt tctgccgtca caggggcgat gaccggaaag
     1381 ctgacagcct tgaatcgctg gcttcggaaa tgatatcccg catcctgagg ctctacgccg
     1441 gaaacggtgt atggaacagt atatatcccg gaaacaggac aatagaagta cggcattgcc
     1501 tcgattttgc gtttttcgga aaatatattc ctcacgatgt accggaaaag atcaggaagg
     1561 aaatgataac tttcctctat gatgaactta ttactgatca ctggatgagg gcacagtcat
     1621 tgtttgatgt tgccgctgaa aattccgacc gtgccgatca tggacctttt ggtgcttttg
     1681 acggttggcc ggtcgacata atggatgcac ttaccgggat gggatttcct gacaaggccc
     1741 ttgagtttta cagggctgtt gaaccggtaa ccaatgaggg aatctgggca caggcacatg
     1801 aattatgggg agaaaacaaa agaaataaaa atgcattggt caggattacc gaaagagact
     1861 ggtgtaacag ggaatcttca tcgggaacag cttttgccca ggttgtgctg aaggatttcc
     1921 tgggtttcta tcccgaagca gacggagagg ttctgaagtc acggggagat tttcagtttg
     1981 acggcaaact gcatcatgtt ctttacagga ataattatta cacccttgaa tatgaatcag
     2041 agaaagcggt aatgaaaaaa gagaaaatca ggaaaaagta aaagccccaa gaggaccgtt
     2101 aaaactcctg aaaaatgaat aaaaataaaa atatatctgc cttttatacc attatgctcc
     2161 tgccgggtct tctgctcttt ctgactgccc ccctgaccgc acagaacgtg atcaggaagg
     2221 cagaggatca ttttgacagg tttaccaatg tggcaggggg atggtgtgcc ggtgacgcga
     2281 ctatttccct tcctcttcct gacggaaaga ccatatggct gttcggtgac agctttattg
     2341 gcaggaaaag cggtaaatac agcatcattt catcaggatc ccgcatgatc aataatgccg
     2401 tcatacttga cgatggcact gaaatgacaa ccatacacgg gggaagcgga gacagccctt
     2461 cttccttcat tcccccggca ggggcggata tattctggcc cgagcatgca gtaatggaaa
     2521 atgacattct gaaggtcttt ggggtgagga taaaatttgt tgacaaccag acgccgggat
     2581 ttaattttga agtcgggact acttataagg ctgcttttac ctatcccggt ttcggacata
     2641 tcagtaccac aaaatttaca tccgtcacgg acagcacctt tcgtttcggt gcctgcgtcg
     2701 ttaaaagcgg ggaatataca tatattttcg gggtaaagga cacaactgcg ggaatcttta
     2761 cctatcccct gccatatctg gcaagggtaa atgagagcat agatgagcct tggcagtttt
     2821 atgcaggctc cggcaactgg tcttacaact gcagtgaggc tgtaccggtc ggggaccggc
     2881 ccatgagcga atcattctat gtgtatgaga aaaacgggag gttctatctc atcatgcatg
     2941 aaatatggct gaagggagaa ctttttattc ttgaggcaga caggattaca ggtccgtgga
     3001 accgcaggtc atcgggtgga acagaaaaca ggtttgcagt tatttccccg catgccggga
     3061 atattaccta taaccttttt gcacatccgc agtttcagga tgagggagat atcctgatct
     3121 ccttcaatgt aaacacaagc aactttatct cgatttacca ggatacccgt aactacaggg
     3181 cccgtttcta ctggcttgat gttgaagctg cggcagtgac cgtaataccg gacaccatcc
     3241 gtttgtttga tgaccctgtt ggtattattg acctgcacaa cggcaggccg gaaagcaggc
     3301 ttgtttatga tccactgaac ggctgcctga aactcaggga tatttcatcc ccggcattgc
     3361 tggagatatc cggtattgac ggcaggattt acatggaacg ggagatcaga gatgatgcgg
     3421 aaataaactg ccggcacctg cgcggaagaa tgatgattgt ctctctccgt ggaaaatggg
     3481 gtgccgaaac cagaaaattc tttttccgct agccggtatc catatgcctc agatagtttc
     3541 tgccgattaa taagttacga ttttcat
//
LOCUS       DAOSPX010000327         3549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_23706_length_3549_cov_3.658271, whole genome shotgun sequence.
ACCESSION   DAOSPX010000327 DAOSPX010000000
VERSION     DAOSPX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23706_length_3549_cov_3.658271"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLZ75_12555"
     CDS             <1..481
                     /locus_tag="PLZ75_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOS73267.1"
                     /translation="KAKGAAYGFHGGVFLRLGLNKIFLQPELLFSTRTNEYTVTNLKE
                     DLSSLESQKFNKLDLPVMAGLKFGPVRINAGPSASLLINSPGDLIRNPEFNTMYSRMT
                     FGYQAGVGLDLFDLLTIDIRYEGSLKKYQNQIENAVGTRVALDDRPNAFLFSLGIMF"
     gene            789..1823
                     /gene="mltG"
                     /locus_tag="PLZ75_12560"
     CDS             789..1823
                     /gene="mltG"
                     /locus_tag="PLZ75_12560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOS73268.1"
                     /translation="MKHKTAKTLLFLAGFLILAGIVLLINIFFSLHISPGDDDEIVLY
                     IPDRASYPQAMDSIKAHLPVKHPAAFDWIAKRKNYPANVKPGRYVISHRMSYIEVINM
                     FRSGDQVPVNITFNNVRTIYDLAGRVGGRISADSASITGFLENPDNYSADGFNRETII
                     SVFIPNTYKFFWNTGPADFYSRMLREYRRFWNEDRLNKAGETGLDPVEVSILASIVDD
                     EAARSDEKPRIAGVYLNRLKRGIPLQADPTIKFALADFSMTRVLRRHLNVDSPYNTYK
                     YRGLPPGPIGCPTIESIDAVLNAEKHDFIYFVAQADFSGYHHFSRTLAEHNRYAAIYQ
                     RELNKRKIFK"
     gene            complement(1816..2835)
                     /gene="murB"
                     /locus_tag="PLZ75_12565"
     CDS             complement(1816..2835)
                     /gene="murB"
                     /locus_tag="PLZ75_12565"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOS73269.1"
                     /translation="MLYIKSYSLKKNNTFGIDCTADNFVIVHTEREAAEFFRKAGRAK
                     EEVFIIGEGSNLLFTSDFHGTIVRSAIRGIRNEWQDDEFVIISAGSGIVWDSFVEWTV
                     KKGYQGLENLSFIPGLTGAVPVQNIGAYGAEAGESVVRLSAVSTTDGTKRIFENHECG
                     FGYRNSVFKNELKGKYLITRVFFRLRKNPHYNLDYGNLREEADRLGGPSVQNIRKAVI
                     KIRRSRLPDPAVSGNAGSFFKNPVVSPVFAGELKKAFPGLPVYNDPSGGRKLAAGWLI
                     EQCGWKGRRSGDAGVHHSQSLVLVNHGNATGADIYRLSEEIRKSVKAKYGLDLEREVE
                     VLGLI"
     gene            2937..>3549
                     /locus_tag="PLZ75_12570"
     CDS             2937..>3549
                     /locus_tag="PLZ75_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS73270.1"
                     /translation="MNCICHDQRVLKIAGVVNDLGCNVTIIGRRKGDCCRESDLPFRT
                     FRFRMLFNRGFLFYSFFNIRLFFYLLFHNYDVMIANDLDTLLPNFLVAVLKRRSLVYD
                     SHEYFTGVPELTGRIFVTWVWKTIESYIFPRLKHVMTVSDSLALQYEKEYGIRPVTVR
                     NCSVSSAGIEGYSGNETGIKAGDLLLIFQGGGINIDRGGEELVE"
BASE COUNT          933 a          807 c          796 g         1013 t
ORIGIN      
        1 aaaggcaaag ggtgcggctt atggttttca cggaggagta tttctcagac tgggattgaa
       61 taaaatattc ctgcagcccg aactgctttt ttccacgagg acaaatgaat ataccgtgac
      121 aaatctgaag gaggatcttt catcactgga atcacaaaag tttaataaac tggatcttcc
      181 ggtaatggca ggcctgaaat tcggaccggt aaggatcaat gccggtcctt ctgcatcact
      241 gctcattaat tcaccgggag accttatcag gaatcccgag ttcaatacaa tgtattcaag
      301 gatgaccttt ggctaccagg ccggagtcgg actggatctg tttgacctgc tgaccataga
      361 tatacgctat gagggcagcc tgaaaaaata tcagaatcag atagaaaacg ctgtcggaac
      421 cagggtagcc ctggatgacc ggccaaatgc atttcttttc agcctgggga taatgttctg
      481 aggaacatgg gagtaaaaga agggtcgtgt cagagcactg accctttttt ttaattcatc
      541 tgaccggatc ctgctgtatt aaatacggaa atacttcctg ttgcctgtct cataaaggcc
      601 tggtccgtca tccattccgt atgctgtccg ggatacctgt atgtgtctta gtgttataaa
      661 tccggataaa gggatgttga aggccccggg gatccctgta ctgattccgg ccattatatt
      721 gcttcattat tgggaccacg gtttgatctt tctgacaata tccctaattt tgccttcttc
      781 ggaaaaagat gaaacacaaa acagccaaga cactgctttt cctggcggga ttcctgattc
      841 tggccggaat tgtactgttg atcaatattt ttttcagtct tcatatttct cccggtgatg
      901 atgatgagat tgttctttat atcccggata gagcttccta tccgcaggcc atggattcaa
      961 taaaggctca tcttcctgtg aagcaccctg ccgcctttga ctggatcgcg aaaaggaaaa
     1021 attatccggc caatgtaaag ccgggccgct atgtgatcag tcacagaatg agttatattg
     1081 aagtaataaa catgtttcgc tcgggcgacc aggttcccgt gaatattaca ttcaacaacg
     1141 tacgtacaat atatgatctt gccggcaggg ttggcggccg tatcagtgct gattccgctt
     1201 caataacagg gtttctggag aatcctgata attattcagc tgacggtttt aacagggaaa
     1261 ccattatttc cgtttttatt cccaatacct acaaattttt ctggaatacc ggaccggcag
     1321 atttttattc aaggatgctg cgtgaataca ggagattctg gaatgaggac agacttaaca
     1381 aggcagggga gaccgggctt gatcccgtgg aggtatcaat tcttgcatcg atagttgatg
     1441 atgaggctgc aaggtctgat gaaaaaccca ggatagcagg cgtttatctg aacaggctga
     1501 aaaggggtat tcctcttcag gctgatccca caattaaatt cgcacttgct gatttttcaa
     1561 tgaccagggt tctcaggcgc catctcaatg tcgattctcc ctataacacc tacaagtaca
     1621 gggggcttcc tccgggtccc atcggctgcc cgacaattga gagtattgat gcggtgctga
     1681 atgccgagaa acatgatttt atttattttg tggcacaggc cgatttttca ggttatcatc
     1741 atttttcccg gacattggcg gagcataaca gatatgccgc aatctatcag agagaactta
     1801 acaagaggaa aattttcaaa taagacccag cacttcaact tcacgttcaa gatcaagtcc
     1861 gtattttgct tttacggatt ttctgatttc ctcagacaac ctgtagatat ccgcaccggt
     1921 ggcattcccg tgattcacca gtaccaggga ctggctgtga tgaactcctg catccccgct
     1981 tctccgccct ttccagccgc attgttcgat aagccagcct gcagcaagct tccttccgcc
     2041 tgagggatca ttgtaaaccg gcaggccggg aaaggctttt ttaagctctc cggcaaaaac
     2101 aggactgaca acgggatttt tgaaaaaact gccggcattt cctgatacgg caggatcggg
     2161 cagcctgctt ctcctgattt ttataacggc cttccttata ttctgaacag aaggccctcc
     2221 gagtctgtct gcctcttccc tcaggtttcc gtaatccaga ttgtaatgcg gattcttcct
     2281 gagcctgaaa aacacccttg ttatcagata ttttcccttc aattcatttt taaaaaccga
     2341 attcctgtat ccgaagccgc attcatggtt ttcaaatatc ctttttgttc cgtcagttgt
     2401 gctgacagca ctgagcctta caaccgattc tcccgcctca gcgccgtatg ctccgatatt
     2461 ctgtaccgga acagcaccgg tcagaccggg tatgaaggaa aggttttcca ggccctgata
     2521 accctttttc acggtccatt caacaaagct gtcccacact atcccggaac ccgccgagat
     2581 aattacaaat tcatcatcct gccattcatt cctgattccc ctgatggctg atctgacgat
     2641 tgttccgtga aaatcagaag tgaacagcag attgctgccc tcaccgatta taaaaacttc
     2701 ttctttagcc cggccggctt tcctgaaaaa ttcagcagct tccctttcgg tatgaacaat
     2761 cacaaaattg tctgccgtac agtcaatccc gaaggtgtta ttctttttta atgaatatga
     2821 tttaatatac agcattgctt ttacaaccag ggcaaagata attattactg aatcaatatt
     2881 gctaactttg cctcatgccc cgctcagatg ataaaatcaa agcagttttt tcagtcgtga
     2941 actgtatctg tcacgaccag agggttttga agatagctgg tgtggtaaat gatctgggat
     3001 gcaatgtcac aatcatagga agacggaaag gagactgctg cagggaaagt gatctcccat
     3061 tcaggacatt cagattcaga atgttgttta acaggggatt tcttttctat tcatttttca
     3121 acatacgtct gttcttttac ctgcttttcc ataactatga tgttatgata gcaaacgacc
     3181 tggatacatt gctgcccaat ttcctggttg cagtactgaa acgcagatcc ctggtatatg
     3241 attcacatga gtattttact ggtgttccgg aactgacggg aaggatattt gtgacgtggg
     3301 tctggaagac catcgaaagt tatatttttc cccggctgaa gcatgtaatg acggtaagtg
     3361 attcactggc actgcagtat gaaaaggaat acggcattcg tcctgtcacg gtgagaaatt
     3421 gctctgtatc atccgccggc attgaaggat attcaggaaa tgaaaccgga ataaaggccg
     3481 gggatcttct gctgattttc cagggcggag gtatcaatat tgacagggga ggggaggaac
     3541 ttgttgagg
//
LOCUS       DAOSPX010000328         3531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_23856_length_3531_cov_4.006617, whole genome shotgun sequence.
ACCESSION   DAOSPX010000328 DAOSPX010000000
VERSION     DAOSPX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23856_length_3531_cov_4.006617"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PLZ75_12575"
     CDS             <1..1243
                     /locus_tag="PLZ75_12575"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF004937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOS73271.1"
                     /translation="NSMKDFFEDKISDRLGGKSFGKSTAVYKFELIKRAKAAAKLAHP
                     DIRLIDMGVGEPDWPADELVVKVLSEEAGKPENRWYADNGIPEFQEAAARYLGKVYGL
                     SGINPHENIVHGIGSKPVLAMLPLCFINPGDVLLTTVPGYPVTGTYTKYLGGEVFNLP
                     LYESNSFLPDLDSIPGTVLKRARLLYINYPNNPTGAVAGREFFDKVVAFAGKHGIVVA
                     HDIAYGALTYDGYRPLSFLSADGAMDVGIEIHSLSKAFNMTGWRMGFVCGNSRAVSAY
                     ASVKDNTDSGQFRAIQKAGIAALENTEITERTVEKYSRRFDLLVNALNEAGFSASKPK
                     GSFYCYVKAPVGAGNGKTFSSASEVSEFLIREAQISTVPWDDAGSYLRFSVTFEAASA
                     DEEKEVTDELVRRLKLLNLRF"
     gene            1294..2073
                     /locus_tag="PLZ75_12580"
     CDS             1294..2073
                     /locus_tag="PLZ75_12580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS73272.1"
                     /translation="MTKISGVIISYNEEKHIGKCLASLQGIADEIIVVDSFSDDSTRD
                     VCSRFNVRFIEQAFKGYVEQKNSALGFAGNRYVLSLDADEELSEELRESILKIKDNPE
                     YDGYQFNRLTNYCGKWMKYSGWYPDRHIRLFDTEKGRWAGTNPHDKIRMNTGSRVTRL
                     RGDILHWYCETFEEHMDKSNHFSSIMAEEYFRQGKKSGILSASFHKWWRFIRTYFIGA
                     GFLDGFRGHAACSISAQASYLKYIKLLQLNRDAKKTNSGHF"
     gene            2042..2632
                     /locus_tag="PLZ75_12585"
     CDS             2042..2632
                     /locus_tag="PLZ75_12585"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOS73273.1"
                     /translation="MQRKQTAGTFEEDIQKSLAVLKNGGVIVYPTDTVWGIGCDATRD
                     DAVNKLQGIKLRNGKKSLIVLVSGLPMLERYVREIPEIAYELLEVSDRPLTIIYPEGR
                     NLAAGIYAGDNSVAIRICKEPFVNVLIDRFRKPLVSTSANFSTKPFPSNFDEIDPALL
                     KKADYVVRYRQDDRKQYRPSSLIRVELSGVISIIRD"
     gene            complement(2699..3229)
                     /gene="rimM"
                     /locus_tag="PLZ75_12590"
     CDS             complement(2699..3229)
                     /gene="rimM"
                     /locus_tag="PLZ75_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOS73274.1"
                     /translation="MTFSDKILLGKITRPFGFDGAVTVKIEKIFQDKIPEFKAVFLEI
                     EGKAVPFIVSSAEKPASTLLRLVFEGYETLEKIREFIGCRIFLPSVRTRAKEGADLSG
                     LTGYKITDTAGGPPGTIQEIIENPGQILLGVQPDEGKYFYVPFHEDLIVKIDNRKKII
                     IMDLPEGLREINDPVS"
     gene            complement(3251..>3531)
                     /locus_tag="PLZ75_12595"
     CDS             complement(3251..>3531)
                     /locus_tag="PLZ75_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOS73275.1"
                     /translation="LAAEKRINEARAAKLAKKQAELAAKARAESQAEDAPEAPAAEAA
                     PEAPAAETAVEAPSAEDAPEVPAAEAAPEASSGETAPDTTSDEKPAEG"
BASE COUNT          978 a          810 c          855 g          888 t
ORIGIN      
        1 aaattcaatg aaagactttt ttgaagacaa gatatccgac aggcttggcg gaaaaagttt
       61 cggcaaatca acggctgttt acaagtttga actcatcaag agggcaaagg cagctgcaaa
      121 gcttgcccat ccggatataa ggctgataga catgggtgtg ggtgaacccg actggcctgc
      181 cgatgaactg gttgtaaaag tgttgtcgga agaggcagga aagccggaaa accgctggta
      241 tgccgataac ggaataccgg aattccagga ggcagcggca cgctatctcg gaaaagtata
      301 cggactatcc ggaataaacc cacatgaaaa tatagtgcac ggaataggtt caaaacctgt
      361 ccttgccatg ctgcccctgt gctttatcaa tcccggggat gtgctgctca ccactgttcc
      421 gggttatcct gtcacgggta catatacaaa ataccttggc ggagaagtct ttaacctgcc
      481 cctttacgaa tcaaactcct ttctccccga ccttgactca attcccggta cggttctgaa
      541 aagagccaga ctcctttata ttaattatcc caacaatccg accggcgctg ttgccggaag
      601 ggaattcttt gataaggtgg tggcctttgc cgggaaacat ggcattgttg tggcacatga
      661 catagcatat ggtgccctta cctatgacgg ttaccggcct ctcagcttcc tgtcggccga
      721 cggggcaatg gatgtgggta ttgaaattca ttccctgtcg aaggcattca acatgacagg
      781 ctggagaatg ggttttgtat gcggaaacag cagagccgtc agtgcctatg ccagtgtcaa
      841 ggacaacact gattccggtc agttcagagc catccagaaa gccggcatcg ctgctcttga
      901 aaatactgaa atcaccgaaa ggacagttga aaagtattca cgcagattcg accttctcgt
      961 aaatgcactc aatgaagcgg gattcagcgc ctcaaagcca aaaggttcat tctactgcta
     1021 tgttaaagca cccgtgggag ccggaaacgg aaaaacattc agttccgcct ccgaggtatc
     1081 ggaattcctt atcagggaag cacagatctc aacggttcca tgggatgatg caggatctta
     1141 cctgagattt tccgtgacct ttgaagccgc ttcggctgat gaggaaaaag aagtcaccga
     1201 tgaactggtc agaaggctga aattactgaa tctcaggttt taataccgaa aacaataata
     1261 acattctttc ccggtcccaa acaacaaaac aagatgacta aaatatccgg tgtcataata
     1321 tcctacaacg aggaaaaaca tataggcaaa tgcctggctt cattacaggg aattgctgat
     1381 gagattattg tagtcgactc cttttcagat gattcaacga gagatgtctg cagcagattc
     1441 aatgtcagat tcattgaaca agcctttaaa ggctatgttg aacagaaaaa ctcagcattg
     1501 ggatttgccg gcaacagata tgtgctgtcg cttgatgcag atgaggaact cagtgaagaa
     1561 ctgagggaat ccatacttaa aataaaggat aatcctgaat atgacggata tcagttcaac
     1621 cggctcacca attactgcgg caagtggatg aaatattccg gatggtaccc cgacaggcat
     1681 atcagattgt ttgatacaga aaaaggcagg tgggcaggta caaaccccca tgacaagatc
     1741 aggatgaata cgggatccag ggtaacaagg ctgcgtggcg atattctcca ctggtactgt
     1801 gaaacctttg aagaacatat ggataagagt aatcattttt cctcgatcat ggcagaggaa
     1861 tatttcaggc aggggaaaaa aagcggaatc ctctctgcct cctttcataa atggtggcgc
     1921 tttatcagaa catatttcat aggagcaggc tttcttgatg gtttcagggg ccatgcagcc
     1981 tgttccatct cagcccaggc cagctatctg aaatatatca aactgttgca attgaacaga
     2041 gatgcaaaga aaacaaacag cgggcacttt tgaggaagat atacaaaaat cactggccgt
     2101 tctgaaaaat ggcggggtca ttgtctatcc caccgatacg gtctggggca ttggctgtga
     2161 tgcaacacgt gacgatgcag tgaataagct gcaagggata aaattgagaa acgggaagaa
     2221 aagcctgatt gttcttgtaa gcgggcttcc catgcttgag agatatgtaa gggagattcc
     2281 ggagatagcg tatgaactcc tggaagtgtc ggacaggcct ctcacaatca tttatcccga
     2341 aggaagaaac cttgcggcag gaatatatgc cggggataat tccgttgcca taagaatctg
     2401 caaagaaccc tttgtaaatg tactgattga cagattccgg aaaccactgg tatccacttc
     2461 ggcaaatttc agcaccaaac cctttccctc aaattttgac gagattgacc cggctctcct
     2521 gaaaaaagcc gactacgttg tgagataccg gcaggatgac aggaagcaat acaggccttc
     2581 atccctgatc agggtggaac tcagtggtgt aatcagcatc atcagggatt agctcagccg
     2641 gtgatttcat atttcaggga cagcattcct gcagccgttt caggatgaac agcggctatc
     2701 agcttacggg atcattgatc tcccgcaatc cctcaggaag atccattatg attatttttt
     2761 tcctgttgtc gatctttact ataaggtctt catggaaagg cacatagaaa tatttgccct
     2821 catccggctg aacaccaaga agtatttgtc ccggattttc aattatttcc tgaatggttc
     2881 ccggcggacc gccggctgtg tcagtgattt tatagcctgt aagccctgaa agatccgctc
     2941 cttccttagc cctggtccgg acagaaggca gaaaaatcct gcaaccgatg aattcacgga
     3001 tcttctcaag ggtttcataa ccttcaaaaa caagtctgag cagagtgctg gcaggttttt
     3061 cagccgaaga aacaataaaa gggacagcct tgccttcaat ttcaaggaat actgccttaa
     3121 attccggtat tttatcctgg aagattttct caattttgac agtgacggca ccatcaaagc
     3181 caaaaggcct ggtgattttc ccaagtagta ttttgtcact gaaagtcatc ggatctggga
     3241 tccggaatac ttatccttcc gcgggttttt cgtctgaagt ggtatccggt gctgtttcgc
     3301 cggaagaagc ttccggtgct gcctctgcgg caggtacttc aggtgcatct tctgcggagg
     3361 gagcctcaac ggctgtttca gcagcaggtg cttccggtgc ggcctctgcg gcaggtgctt
     3421 caggtgcatc ttctgcctgg ctttccgccc tggcttttgc cgccagttca gcctgtttct
     3481 ttgccagttt tgcagccctg gcctcattaa tccgcttttc ggctgccagc t
//
LOCUS       DAOSPX010000329         3525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_23916_length_3525_cov_4.705187, whole genome shotgun sequence.
ACCESSION   DAOSPX010000329 DAOSPX010000000
VERSION     DAOSPX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23916_length_3525_cov_4.705187"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="PLZ75_12600"
     CDS             complement(<1..1749)
                     /locus_tag="PLZ75_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOS73276.1"
                     /translation="MNQYSEYAGKDGILLGLDIGSVSLNTVVLDTRFNVIENFYDWIQ
                     GRPFNVLNDRLRSLLTRYPADSFRGTAITGTGGKLASGLTGGIFVNEIIAQATSCGAL
                     YPEAKTVIEIGGEDSKLIILEKDPAGGPSKLVDFEMNSICAAGTGSFLDQQARRIGVS
                     IEKEFGEMSLKSVNPPRIAGRCSVFAKSDMIHHQQIATPLHDIVAGLCFALARNFRSN
                     VGKSREFRKPVIFSGGVAANVGMIRAFREILNLNGDELIIPQYHASMGAIGAVMYAHS
                     NQSGMNGFPELEELEQYLSLTSSSFTSLPRLKESDAVYNTELNFRKNGRERLQVYLGI
                     DVGSLSTNVVLIDREHNVVARRYLPTAGKPLEAIQKGLTEIYDEVGEDVEVIGAGSTG
                     SGRYLTGDFIGADTIRNEITAQATAAIDYDPTVDTIFEIGGQDSKYISIENGVVIDFE
                     MNKVCAAGTGSFLEEQAEKLNINIVEEFGNMALNSESPVKLGDRCTVFMESDLNSFMQ
                     KGARNDNLVGGLAYSIVYNYLQKVVGDRRVGNKIFFQGGVTNNKAVVAAFEEVTGKKI
                     IIPPHFDVTGAIGAAIL"
     gene            complement(1875..2771)
                     /locus_tag="PLZ75_12605"
     CDS             complement(1875..2771)
                     /locus_tag="PLZ75_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS73277.1"
                     /translation="MIRDIIKIDEDLCNGCGLCIPNCHEGALQIIDGKARLVSELMCD
                     GLGACIGYCPEGAISIEKRMAEPYNEKRVMEQMVTKGKNTVTAHLRHLMDYNETGYLN
                     EAIDFLEENRSALAFDPDEVLEEVFSGGKTRKPSLKNQSRPSPEPAPGCCPGFGEMPE
                     PKAAALISESSQSELRHWPVQMHLINPEASYFHRADLLIAADCVAYSMAGFHSRFLKG
                     KSLVIACPKLDRNTEIYVEKLKILIDKARVNTITVMMMEVPCCGGLLHMIKTALAEAS
                     EKVPVKQIIISIRGEILKENWI"
     gene            complement(2827..>3525)
                     /locus_tag="PLZ75_12610"
     CDS             complement(2827..>3525)
                     /locus_tag="PLZ75_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOS73278.1"
                     /translation="PGDDKPSLLTAYEAETLFHEFGHALHQLLSNSTYPGVSGTSVPR
                     DFVELPSQIMEHWVFEPEVLEVYAKHYRTGEVIPAELVDKLEKAGKFNTGFNTLEFLA
                     AALLDMEYHSLTEPISDLDIREFEKQILDKYGMIPEIKPRYRSTYFNHSWSGGYAAGY
                     YSYYWSEMLDADAFQAFKETGDIFSPEVAARFENEILSRGGTRDPVDMYVAFRGKEPG
                     IDALLLNRGLKSER"
BASE COUNT          825 a          894 c          790 g         1016 t
ORIGIN      
        1 cagaatagct gcacctattg cacctgtgac atcgaaatgc ggagggatta tgattttctt
       61 accggttact tcttcaaaag cggccacaac ggctttgtta ttggtgacac ccccctggaa
      121 aaagatttta tttccgaccc tgcggtcacc gacaaccttc tggagataat tgtacactat
      181 tgaataggcc agtccgccga caagattgtc atttctggca cctttctgca tgaaggaatt
      241 aagatccgat tccatgaaaa cggtgcatct gtcgccaagt ttaacagggc tctctgaatt
      301 cagcgccata ttcccgaact cttcaacaat attgatattc agcttttcag cctgttcttc
      361 aaggaaggat ccggtccccg cggcacacac tttattcatc tcaaaatcga tcacaacacc
      421 attctcgatg cttatatatt ttgaatcctg ccctccgatc tcaaatatgg tatcgacagt
      481 gggatcataa tcaatagctg ctgttgcctg ggcagttatc tcattccgga ttgtatcagc
      541 cccgataaaa tcacctgtaa gatatcttcc ggatcctgtt gatccggcac cgatgacttc
      601 aacatcttca ccaacctcat catatatttc cgtcagtccc ttctgaattg cttccagggg
      661 ttttccggcc gtggggagat atctcctggc aacaacatta tgttccctgt ctatcagcac
      721 aacattggta ctgagagagc ccacatctat tccgaggtag acctggagtc tttccctgcc
      781 gttcttccgg aaattcaact ctgtattgta aactgcatca gattctttca gcctgggaag
      841 gctggtgaac gatgaagagg tcagggagag gtattgttcc agctcttcca gctccgggaa
      901 gccgttcatg cctgactggt tggaatgagc atacatgaca gctccgattg ctcccattga
      961 tgcatggtat tgcgggatga tgagctcatc cccgttcagg ttcaggattt ccctgaaggc
     1021 tctgatcatg cccacattgg ccgctacacc gcctgaaaag ataaccggtt tccggaattc
     1081 cctgcttttg ccgacgttgc ttctgaagtt cctagccagt gcgaaacata atccggcaac
     1141 aatatcatgg aggggagtgg ctatctgctg gtgatgaatc atatcacttt ttgcgaaaac
     1201 gctgcatcgc ccggcgattc ttggcggatt gaccgatttg agggacatct cgccgaattc
     1261 cttctcaatg gatacaccta ttctcctggc ctgctggtca aggaatgacc cggtccccgc
     1321 ggcacagatg ctgttcatct caaaatccac cagttttgac ggacctcctg caggatcttt
     1381 ttcgagtatg atcagtttgg aatcttcacc gccaatttca attacagtct ttgcttccgg
     1441 ataaagcgca ccgcacgaag ttgcctgtgc aatgatctca ttcacaaaaa tacctccggt
     1501 caggccggaa gccagctttc cgcctgttcc ggtaatggca gttcccctga aagaatctgc
     1561 cggatatctg gtaagcagtg atctgagcct gtcattcagg acattgaatg gccgtccttg
     1621 aatccagtca taaaaatttt caattacatt aaacctggtg tcaagaacga ctgtattcag
     1681 cgacaccgat ccgatatcaa gtccgagcag tataccatcc ttacctgcgt attcagaata
     1741 ctgattcata aggcggttgt attatctggt ctgttacctg aataaaaccg gcctggtaaa
     1801 taatgggttc cggttaatca aatttatgaa atcttccgga aaaatattta atcggatgcg
     1861 gattttttga ggtctcagat ccagttttct ttcagtatct ctccccggat gctgatgatg
     1921 atctgtttta ccggtacttt ttctgatgcc tctgccaggg cggtttttat catgtgcagt
     1981 aatcctccgc agcagggaac ttccatcatc ataacagtga tggtattcac ccttgctttg
     2041 tctatcagga tttttaattt ctccacgtat atctccgtat tgcggtcaag tttcggacag
     2101 gctataacga gtgatttacc cttaaggaac ctgctgtgaa accctgccat tgaatatgcc
     2161 acgcagtcgg cagcgatcag caggtcggcc cggtggaaat atgaagcttc ggggtttata
     2221 aggtgcatct gaaccggcca gtgccggagc tcggattgcg atgactcact aattaatgcg
     2281 gcagccttag gttccggcat ttccccgaag cccgggcagc atcccggggc cggttccggg
     2341 gaagggcggc tctgattttt tagggagggc ttccgtgttt tgccaccgga aaatacctct
     2401 tcaagcactt cgtcaggatc gaatgccagg gcagatctgt tttcctccag gaaatcaatt
     2461 gcctcattca ggtagcctgt ttcattataa tccatcagat gccggagatg agcagtcaca
     2521 gtatttttcc ccttagtcac catctgctcc atcactcgtt tctcattgta tggttccgcc
     2581 atcctttttt ctatactgat ggctccttca gggcagtatc ctatgcatgc ccccagaccg
     2641 tcgcacatga gctcacttac cagccttgcc tttccgtcaa tgatttgcag ggctccctcg
     2701 tggcagttgg ggatgcataa tccgcatccg ttgcacaagt cctcgtcaat ttttattata
     2761 tcccttatca tggccgcaat tttacggata agcggtgaaa gtttgcggga aacatgattt
     2821 ttgtcatcac ctttcagatt tcaatcccct gttaagaagc agggcatcta ttccgggttc
     2881 ttttcccctg aaggcaacat acatatcaac gggatccctg gttccgcccc ttgacagaat
     2941 ctcattttcg aacctggctg ccacttccgg actgaatatg tcgcccgttt ccttaaatgc
     3001 ctggaatgca tcggcgtcga gcatttcgct ccagtaatag ctgtaatacc ctgcagcata
     3061 tcctcctgac caggaatggt tgaagtaggt tgatctgtat ctgggtttta tttcggggat
     3121 cattccgtac ttgtcaagta tttgtttttc aaattccctg atatccagat cggatatggg
     3181 ttcggtaaga ctgtgatact ccatgtcgag cagggctgcc gccaggaatt caagcgtatt
     3241 gaatccggta ttgaacttgc ctgccttttc aagtttatcc accagttcag cggggattac
     3301 ctcgcccgtc cggtaatgtt ttgcatacac ttcaagcact tcgggttcaa aaacccagtg
     3361 ttccattatc tgtgaaggca gttcgacaaa atcgcgggga acagaggtgc ctgagactcc
     3421 cgggtatgtt gaattggaca gcaactgatg aagggcatgt ccgaattcat gaaacagtgt
     3481 ttcggcttca tatgctgtaa gaagggaggg tttatcatct cccgg
//
LOCUS       DAOSPX010000330         3523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_23931_length_3523_cov_3.380623, whole genome shotgun sequence.
ACCESSION   DAOSPX010000330 DAOSPX010000000
VERSION     DAOSPX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23931_length_3523_cov_3.380623"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1307
                     /gene="pdxB"
                     /locus_tag="PLZ75_12615"
     CDS             183..1307
                     /gene="pdxB"
                     /locus_tag="PLZ75_12615"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF001309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HOS73279.1"
                     /translation="MKIIADDKIPFLKGVLEPFAEVIYLPGHRIDNRILQDADALLVR
                     TRTRCNEALLRNTGIKFIATATIGYDHIDADFCNSKGIAWTNAPGCNSSSVRQYVAAA
                     LLRISGQFRYRLKNKTLGIVGVGNVGSKVSSLARILEMNVLLNDPPRARKEGDEGFAG
                     LEQVLMESDIVTAHVPLNPAGEDSTVNLFNEDCFRMMRKGTWFINTSRGEVTDTLALK
                     NALDSGKLMGAVLDVWEKEPDIDTELMEKTFISTPHIAGYSADGKANGTSMAVNALCE
                     FFGFPLKDWYPDNVPAPDAPVILVNCRGKTDEDVMREAVLHTYNIDDDSVRLKQMPSG
                     FEGQRGDYPLRREFPAYTVILKGGTRNTRMMLRDLGFKVV"
     gene            1450..2388
                     /locus_tag="PLZ75_12620"
     CDS             1450..2388
                     /locus_tag="PLZ75_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS73280.1"
                     /translation="MKKQSRRSFIGKLAVAGAGTAAAGIGTAGLISCKPGVKSSDTPG
                     GVFKRRYKLSLNAYSLNQPLRDGSMNLFDLLEFSSENGFDGVDLTGYYFPGYPEVPSD
                     EYLYSVKKRAFQLGLFINGTGIKNDFTWSDRSKREEGKELIRNWVIVAEKLGAPSLRI
                     FTGNLSKEEFSWEERAEWIAEDIRECAGFGKKHGVMMAVQNKNDFISDASHVDKMFKM
                     INHDWAGLMLDMGSYRVPDPYAEMASNSKYAISWLIQEKIYINDQQTDPDYGRIRDMV
                     RECGYQGFLQLETLGEGDPVVKIRTLFDKVNSVFNS"
     gene            complement(2473..>3523)
                     /locus_tag="PLZ75_12625"
     CDS             complement(2473..>3523)
                     /locus_tag="PLZ75_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73281.1"
                     /translation="ICVTGSFIKKLREPGSDPSKMSFFMPDHNGPCRFGQYNHFHRLL
                     FDRLGYKDAELITPSNDTSYADLAGDHGLKFRMNAWKGFIAADFLRKIYRETRPYEIN
                     KGDCDRLYERSLKRLEICIEKGTRGLRKILTEIAEEFHAVPANKNVRKPVVAVIGEIY
                     MRDNAGCNGNIANRLEDLGVEVVIGPFSEWIYYSTHRFTRDSKWKKDTKGIIRSRIQG
                     FGQDVIANTLLRNVKNFTDHEKDVSLHDMLNLCNTYVSEFYDGDPPIAMGTSVALSGR
                     GVSGMAAILPFTCMPGTLISSVSDSFRKDHNNIPFINIAYDGQDTVSLDTMLQAFVFQ
                     AKEYAASLTARTVSF"
BASE COUNT          982 a          721 c          901 g          919 t
ORIGIN      
        1 gaaagaacgt gagactcctc tgagcagatc gcacagaaaa ttgttatctt tagatggtat
       61 tattctgatg aaccaggccc atgactgttg tttcgggggc tcagatattc attcagaatg
      121 atctgccggt acgggaattg attccggccg cataactcat tcatacataa catataacgc
      181 ggatgaaaat aattgccgat gataaaattc cttttctgaa aggagtcctg gaaccctttg
      241 ctgaagtgat atatcttccg ggtcatcgga ttgacaacag gatactgcag gatgctgatg
      301 cgcttctcgt gcggacaagg acgagatgca acgaggccct cctgagaaat acgggaataa
      361 aattcattgc caccgcaact atcggctatg atcacataga tgcggatttc tgcaacagta
      421 agggcatcgc atggaccaat gccccgggct gcaattcatc gtcggtccgg caatatgttg
      481 cagctgccct tctgagaata tctggacaat tcaggtacag gctgaaaaac aaaaccctgg
      541 gtatcgtggg tgtagggaat gttgggtcaa aagtcagcag cctggcccgg atacttgaaa
      601 tgaatgtcct tttaaatgat cctcccaggg caagaaaaga aggggatgag ggatttgccg
      661 gccttgaaca ggttcttatg gaatccgaca tcgttaccgc tcatgttcct cttaatcctg
      721 ccggagaaga ctctacggta aacctgttca atgaggactg tttcagaatg atgagaaaag
      781 gtacctggtt catcaatacg tcgaggggag aggtgaccga taccctggcg ctgaaaaatg
      841 cacttgacag cggaaagctt atgggggcag tgctggatgt atgggagaag gaaccggata
      901 ttgatacaga actgatggaa aagacattta tttcgacacc acatattgca ggttattctg
      961 cagatggcaa ggcaaatggc acatccatgg ccgtcaatgc attatgtgaa tttttcggtt
     1021 ttccgctgaa ggactggtat ccggataatg tacctgctcc ggatgctccg gtaatactcg
     1081 tcaactgcag gggaaagacc gatgaagacg tgatgaggga ggctgttctc catacctaca
     1141 atattgacga tgactctgta aggctgaagc aaatgccttc gggatttgag ggacaaaggg
     1201 gggattatcc cctgagacgt gaattcccgg cctataccgt tattctgaag ggaggaacga
     1261 gaaacacccg gatgatgctg agggatctgg gatttaaggt agtctgacag ggtgcagggg
     1321 cggagcgccg ggagcagggg cagggtgcaa ggtgtgccgg gaaaatggtt atgccggaga
     1381 tggggagata aaggaatagg atggtgatga atatttgagg gaataatcaa aaaaacagga
     1441 tttatgtata tgaagaaaca aagcagaaga agttttatcg ggaaacttgc agtggcagga
     1501 gcaggaacag cagctgcagg gataggaaca gccggattaa tctcctgtaa accgggggtg
     1561 aaatcctcag ataccccggg cggagtcttt aaaagaaggt acaagctcag tctgaatgca
     1621 tattccctga atcagccctt aagggacggc agtatgaacc ttttcgacct tctcgagttc
     1681 agttcggaaa acggttttga cggcgttgac cttacggggt attatttccc gggttatcct
     1741 gaagtgccat ctgatgaata cctgtattca gttaaaaaga gagcttttca gctgggcctt
     1801 tttatcaacg ggaccggaat aaaaaatgat tttacatggt ccgacaggtc aaaaagggaa
     1861 gagggaaagg aacttatcag aaactgggtg atcgttgcgg aaaagctggg agctccgtcg
     1921 ttgaggattt ttaccggtaa cctttcaaag gaggagtttt catgggaaga gagggcagaa
     1981 tggatagcgg aggatatacg ggaatgtgcc ggatttggta aaaaacacgg ggttatgatg
     2041 gctgttcaga ataaaaatga ttttatcagc gatgcttcgc acgtggacaa aatgtttaaa
     2101 atgataaatc atgactgggc gggcctcatg cttgatatgg ggagttaccg tgttccggat
     2161 ccttatgctg aaatggcttc aaattcaaag tatgccatct catggctgat acaggaaaag
     2221 atatatatca atgaccagca gacggatcct gattacggca ggataaggga tatggtcagg
     2281 gaatgcggat atcagggttt cctccagctt gaaactcttg gtgaaggcga tccggttgta
     2341 aaaatccgga ctctctttga caaggtaaac tcagtgttta attcatgagt cctcagtttt
     2401 actgccagta tacaaaccgg cgctgaatat ttaaacgatt aaccttaaac actccgcaac
     2461 cggcacacaa aactaaaaac taactgtcct ggcagtaagt gaagctgcat actcctttgc
     2521 ctgaaacacg aaagcctgca gcatcgtgtc aagcgacacg gtatcctgtc cgtcataggc
     2581 aatgttgata aagggtatgt tgttgtgatc ctttctgaat gaatcgctta ctgaagaaat
     2641 aagagttccg ggcatacatg tgaatggaag tattgccgcc attcccgaaa cacctctccc
     2701 tgacagtgca actgatgtac ccatggcaat cggcggatca ccgtcataga attcgctgac
     2761 ataggtattg caaagattaa gcatgtcatg aagtgaaaca tccttttcgt ggtcggtgaa
     2821 atttttaaca ttgcggagca atgtatttgc tattacatcc tggccgaaac cctgaattct
     2881 ggatctgatg attccttttg tgtctttttt ccatttactg tcgcgggtga agcggtgtgt
     2941 ggaataatat atccattccg aaaacgggcc aatgaccact tcaacgccca gatcctcgag
     3001 gcggttggct atatttccgt tgcatcctgc attgtcgcgc atatatattt cgcctataac
     3061 tgcaaccacg ggcttgcgta catttttgtt cgcaggtact gcatggaatt cttccgcaat
     3121 ctcagtgagg attttacgca gtcccctggt tcccttttct atacagattt ccagtctttt
     3181 aagtgatctt tcataaaggc ggtcacaatc acctttgtta atctcatagg gtctggtctc
     3241 cctgtatatt tttcttagaa aatcggcagc aataaagcct ttccatgcat tcatcctgaa
     3301 cttcagtccg tgatctcctg ctaaatctgc ataggacgtg tcattggaag gagttatcag
     3361 ttcagcatcc ttgtatccaa gcctgtcaaa gagcaatctg tggaaatggt tatactgtcc
     3421 gaacctgcat ggcccgttat ggtcaggcat gaaaaagctc atttttgaag ggtcactgcc
     3481 aggttcccgg agttttttaa taaagcttcc cgtgacacat atc
//
LOCUS       DAOSPX010000331         3522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_23941_length_3522_cov_3.422267, whole genome shotgun sequence.
ACCESSION   DAOSPX010000331 DAOSPX010000000
VERSION     DAOSPX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23941_length_3522_cov_3.422267"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PLZ75_12630"
     CDS             complement(<1..1192)
                     /locus_tag="PLZ75_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOS73282.1"
                     /translation="MNKNLKYTRNIGIMAHIDAGKTTTTERILYYTGRTHRMGEVHDG
                     NAQMDWMVQEQERGITITSAATTTYWKYNNEDYRINIIDTPGHVDFTVEVERSLRVLD
                     GAVAVFCAVGGVEPQSETVWRQADKYHVPRLAFVNKMDRTGADFFSVVSQIREKLNAH
                     PVPIQIPVGSEENFRGLVDLIRMEARMYSDDASPESGFRTESIPAGLLEEAEEWRGRM
                     VEALAETDETFLERYVDDPGSITTGEILAALRKSVIDGSTVPVLCGASFRNKGIQSLL
                     DAIAAFLPSPVDIGAVRGTDPRNNDEITRDPDENAPFLALAFKIANDPFAGRLVFLRV
                     YSGVLRAGDTVLNVRTGKRERINRLFRMHANKQDKADAISAGDICAAVGFKDLRTGDT
                     LCAIH"
     gene            complement(1217..1693)
                     /gene="rpsG"
                     /locus_tag="PLZ75_12635"
     CDS             complement(1217..1693)
                     /gene="rpsG"
                     /locus_tag="PLZ75_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOS73283.1"
                     /translation="MRKVKPKKRILLPDPRFNDPFVTRFVNNLMFNGKKNTAYKIFYD
                     SLDIIGDKLKEEGKTPLEIFKQALENVTPQVEVKARRVGGATFQVPMEIRPDRKVSIS
                     MKNMILFARKRTGRSMAERLAAELMAAYKLEGGAYKKKEDTHRMAEANKAFAHFRF"
     gene            complement(1712..2086)
                     /gene="rpsL"
                     /locus_tag="PLZ75_12640"
     CDS             complement(1712..2086)
                     /gene="rpsL"
                     /locus_tag="PLZ75_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOS73284.1"
                     /translation="MPTIQQLVRKGRIKLKEKSKAPALDSCPQKRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHLIRGAL
                     DTSGVDGRTQRRSKYGTKRPKK"
     gene            2506..>3522
                     /locus_tag="PLZ75_12645"
     CDS             2506..>3522
                     /locus_tag="PLZ75_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOS73285.1"
                     /translation="MKTYQTDQIKNIVLLGNSGSGKTILAETMLFNSGSIERRGTIEG
                     RNTVSDYRPIEQENGNSIFSTVLFTEYLNKKINIIDTPGLDDFSGGVVSSLFAADLAV
                     MVINVQNGVEVGTEIHFRHAAAVQKPMIMVINGLDHEKANFEKSIEMMKERLSTDVML
                     VQYPLNEGTGFNTVIDVVRMKMLRYGKDGGKAEILDIPGSEAGRAAELHYALVERAAE
                     SDEGLMEQFFANDTLTEEEIRKGIAKGIVSRSLFPVFCISAKNNTGIDSLMDFIVNEA
                     PSITDMPGQKNSRGETVQADPNGPASLYIFKSSIEEHIGEINYFRVFSGKVSENLDVI
                     NTSNG"
BASE COUNT          900 a          868 c          756 g          998 t
ORIGIN      
        1 tgtgaatggc acaaagcgtg tcacccgtcc tgagatcctt gaagcccacc gcggcacaga
       61 tatcaccggc tgatattgca tcggccttat cctgcttgtt ggcatgcatc ctgaaaaggc
      121 ggtttatcct ttcacgcttc cccgtacgta cgttgagaac agtatctcct gccctcagca
      181 ctccggaata taccctcaga aataccagcc gtccggcaaa gggatcattt gcgattttga
      241 aggcaagagc taaaaagggt gcattttcat caggatcgcg ggttatctca tcattgtttc
      301 tcggatctgt tccccttaca gcacctatat caacaggcga cggaagaaac gcggctattg
      361 cgtcgagaag actctgaata cccttgttcc ggaaggaagc tccgcaaaga accgggactg
      421 tgctcccgtc aatcaccgat ttcctgagag ccgcaagaat ttcccctgtt gttattgacc
      481 cgggatcgtc aacatatctt tccagaaatg tttcatccgt ttctgccagg gcctcaacca
      541 tccttcccct ccattcctca gcctcttcca gcagaccggc aggtatgctt tcggttctga
      601 aaccggattc gggtgacgca tcatcactgt acatccttgc ctccatccgg atcaggtcaa
      661 caagtcccct gaagttctct tccgatccaa caggaatctg tatgggaacg ggatgtgcat
      721 tcagtttctc cctgatctga cttacgacgc tgaaaaagtc tgctccggta cgatccatct
      781 tattgacaaa tgccagtctc ggcacatggt atttatctgc ctggcgccat actgtctcag
      841 actgtggctc aacgcccccc actgcacaga atactgcaac ggcaccgtcg agaaccctca
      901 gcgatctttc cacttcaacc gtaaagtcaa catgccccgg cgtgtcgatt atattgatcc
      961 tgtagtcttc attattgtat ttccagtatg tggttgtggc ggcagaagta attgttattc
     1021 ccctttcctg ttcctgaacc atccagtcca tctgggcatt tccgtcatgg acttctccca
     1081 tgcgatgggt acgaccggta taatacagaa tgcgttccgt agtggtggtc ttaccggcat
     1141 ctatgtgagc catgatcccg atgtttctgg tatatttcag atttttgttc atcaatctgt
     1201 ccgcccggct tttatattaa aacctgaaat gggcaaaagc cttgtttgct tcagccattc
     1261 tgtgagtgtc ttcctttttc ttgtatgccc cgccctctag tttgtaagct gccatgagct
     1321 cggctgcaag tctttctgcc atggaacggc ctgtcctttt tcgtgcaaaa aggatcatat
     1381 tcttcatgct tatgctcacc ttgcggtcgg gacggatttc catcgggacc tggaaagtgg
     1441 cacctccgac acggcgtgcc ttgacttcaa cctgtggtgt aacattttcc agagcctgct
     1501 tgaaaatttc aagaggcgtt tttccttctt ccttcaattt gtcaccaatg atgtcgagtg
     1561 agtcgtaaaa aatcttatat gcagtgtttt tcttgccatt gaacataaga ttatttacaa
     1621 acctagttac aaacggatca ttgaacctcg gatccggtaa cagaattctc ttctttggtt
     1681 taacttttct cattactata ttctgtttat gttacttctt tggccttttg gtgccatatt
     1741 ttgaccttct ctgagtacgg ccgtcaactc ctgaagtatc aagggctcct cttatcagat
     1801 gatatctgac accgggaaga tccttcaccc tgcctcccct tatcagcacg atggagtgct
     1861 cctgaagatt gtgcccttca cccggtatat aggcgttcac ttccttctga ttggtaagcc
     1921 tgaccctggc aactttcctc atcgctgaat ttggtttctt gggagtggtg gtgtaaacac
     1981 gcacacatac acccctcttc tgaggacatg aatccagggc aggagcttta cttttctcct
     2041 tcagctttat cctgcctttt cttactagct gttgaatagt cggcatattc tcttttgttt
     2101 tatattaatt tttcacttaa aaaattggtc cgcaaaggta ttcaatttat taataaaaca
     2161 acagacccgg tctgaatttt tccgccacag catggatcac ctcagcattc ccccctgcag
     2221 atgatccttc aggcacatct gaacctttat tccgcttctg tctgtataaa cttgtctgta
     2281 agccttaaac aggttacttc ccgatctttc ccggagaaat ctcttttgta aacttactgt
     2341 tgagggaaat accggtactc ctgcatttca aaaaactggt gcaaagaaaa taaaattttt
     2401 gtaattatgc cttacctgac aggcagtttt taaaaaattg ttaattttgt cagcccaaaa
     2461 cagggaatgt ttaattaatt acctactaaa caatttatta taatcatgaa gacataccag
     2521 actgaccaga taaaaaacat tgtgcttctg ggcaactccg gatcaggtaa aactatcctg
     2581 gcagaaacaa tgctttttaa cagtggttca attgaaagaa gagggacaat tgaggggaga
     2641 aacactgtat ctgactaccg gccgatagaa caggaaaacg gaaattcgat cttttcaaca
     2701 gtgcttttca cggaatatct caataagaaa ataaatataa ttgacactcc cgggcttgac
     2761 gatttcagcg gtggtgtcgt ttcctcactt tttgcagccg acctggcagt aatggtaata
     2821 aatgttcaga acggggttga agtaggtacc gagattcatt tccggcatgc cgccgcggtt
     2881 cagaaaccaa tgataatggt gatcaacggt ctggatcatg aaaaggccaa cttcgaaaaa
     2941 tccatcgaaa tgatgaagga aagactgagt actgatgtca tgcttgtaca ataccctttg
     3001 aatgaaggaa caggtttcaa cacggtgatc gatgttgtca ggatgaaaat gctccgttac
     3061 ggaaaggacg gcggcaaggc cgagatcctt gacattcccg ggagcgaggc cggcagggct
     3121 gccgagcttc actatgcact ggttgaaaga gcggcggaaa gtgatgaggg actgatggaa
     3181 cagttcttcg ccaacgatac cctcactgaa gaggaaataa gaaaaggcat tgctaaggga
     3241 atcgtttcga gaagcctgtt cccggttttc tgtatatcgg cgaaaaacaa taccggaata
     3301 gactccctga tggatttcat tgtaaatgaa gccccctcca tcaccgacat gcccggacag
     3361 aaaaacagca ggggagagac ggtgcaggcg gatccgaacg gccccgcttc cctctatatt
     3421 ttcaaatcct ccattgagga acatatcggg gaaataaatt acttcagggt cttttccgga
     3481 aaagtcagcg agaatctcga cgtcataaac accagcaacg ga
//
LOCUS       DAOSPX010000332         3508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_24066_length_3508_cov_4.214306, whole genome shotgun sequence.
ACCESSION   DAOSPX010000332 DAOSPX010000000
VERSION     DAOSPX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24066_length_3508_cov_4.214306"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLZ75_12650"
     CDS             <1..212
                     /locus_tag="PLZ75_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS73286.1"
                     /translation="LSCAGASPVYKLLTGETFPADDMPPLNSPVMSRIGYHIRTGGHA
                     VTLYDWQQYLRFADMHLQKKERATF"
     gene            877..1527
                     /locus_tag="PLZ75_12655"
     CDS             877..1527
                     /locus_tag="PLZ75_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73287.1"
                     /translation="MKKNKEFDNNQADGSIVFPFKPISEWDGKQGIHNNEDIPEWGLA
                     ERLSRGYGLSRERISHLIRYVVYAFGTSSYFEEEIKVLDFEKNREPILSVRFESCNRQ
                     ITIRDPKIIDKLYKLLLGENNLFKLQKPFDKEIIKQIAIEVLKDITGKDGVQEQTAYC
                     IIGFIFAYHGYGICKEKIMTEGEFIYDTIDKESKSILVKETYTEYLARRIREYVTD"
     gene            complement(1628..1701)
                     /locus_tag="PLZ75_12660"
     tRNA            complement(1628..1701)
                     /locus_tag="PLZ75_12660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1665..1667),aa:Arg,seq:acg)
     gene            2003..>3508
                     /locus_tag="PLZ75_12665"
     CDS             2003..>3508
                     /locus_tag="PLZ75_12665"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOS73288.1"
                     /translation="MKKPLKILLLSLSGLILLVFILLVSVPVIFRDEIKAKVEQIINE
                     SVYATVNVQDYRLNFFRNFPNLALGLDNVSVVGIGKFENDTLAAFRSLDLVFYLPSVF
                     KKTGYEIRSIVIDKALLTVTYLDDGSFNWDISRDTASVATDAADAESSDMKILLKKVK
                     LVNSTLVYNDYGLDMKALINDLSFLLSGDMTASETDLKIKLTAGDVNFIMDGIKYLNG
                     ARAKGELDLSANLDDYKFTFRENHLTINDLKINFSGWVAMPEDDIETDLTFKSEQTSF
                     KTLLSLIPAVYMHDYRDLKAAGNFSFSGSARGIYSDADSTMPDIDLRLVVNDGLVSYP
                     ALPEKISSIDLKAEAFVDGKDMDNTVVNIEKFHMELAGNPFDFGFALKTPLSDPDIKL
                     SMNGKLDLDALSNAIPLDSMDLSGLVDVSLMLAGQLSMIDRQQYDDFRASGKLSVKNM
                     RVEMTGYPEIRVNNAGFEFTPAYAALPGADLLIGENSDFSLSGRLENYIPYI"
BASE COUNT         1123 a          663 c          744 g          978 t
ORIGIN      
        1 tcctttcatg tgcgggagca tcgccggtat ataaactgct taccggggaa acatttccgg
       61 cagacgatat gccaccgctc aattcacctg taatgagcag gatcggatac catatcagaa
      121 cgggaggaca tgctgtaacc ctgtatgact ggcaacagta tctcagattc gccgacatgc
      181 atctgcagaa aaaagaaagg gcgacatttt aagacatgaa gtaaaactat tcgcaaggta
      241 aaaactccaa aaccggctat gccgcccttt aattgctggt ttaatataaa gggaaggcgt
      301 tttgttaccc ggaacatgaa aaaaaattaa agaaaaaagg ccggcagaag aggagaaatg
      361 aaaaaaccta aaaccatgat aacttaaacc catttaaaga tgccggcctc cgcgactatt
      421 attgcagcaa gcttttcaat caacttaatg aaagaggaga ctgaatccaa ccagccaaca
      481 atgcaaaacc aaatcaggtt actaaatcaa attctcaaaa taactttcag tttcctttta
      541 tcaggtgtaa atatataaaa tgttttaaat atatgtcaag ggtatttgtc atactgttat
      601 taacactttt taactagata tcaacataaa aaatataatt ttataagatg ctctgattca
      661 attacttgtc gtactcctct gaaaattgaa ataaaagtct ggttcccggt gcaaccagat
      721 tttttttttt tcgtatgaag taattgattc tgcaaatgat tttcaaaaaa gctcgtgtct
      781 gctatgcttc aggtattcag agttttaatc cggctggaat tctgctttca gaaaggagta
      841 aaggtttcat aatatttata tgataataaa atggcaatga aaaaaaataa ggaatttgac
      901 aacaatcagg ctgacggctc aatagtattt cccttcaagc cgatttccga atgggatggc
      961 aagcagggaa tccataacaa tgaagatatt ccggaatggg gccttgctga gcggctttcc
     1021 cgcggatacg gcctgtcaag ggagaggatc agtcacctga tcaggtacgt tgtttatgca
     1081 tttggaacca gttcctattt tgaggaagaa ataaaagtgc tggactttga aaagaacagg
     1141 gaaccaatcc tttcagtcag gtttgaatcc tgcaacagac agattacaat cagggatccg
     1201 aaaattatag acaaactcta taaattactt cttggtgaaa acaatctttt taagcttcag
     1261 aagccttttg ataaggaaat cattaaacaa atcgcgattg aggttctgaa ggatattacg
     1321 ggcaaggacg gagtgcagga gcagacagct tattgcatta tcggatttat ttttgcatac
     1381 catggctatg gcatttgcaa ggaaaaaatc atgaccgagg gtgaattcat ttacgacaca
     1441 atagacaagg aaagtaaaag cattctggta aaggaaacct atactgaata tcttgcacgc
     1501 aggatcaggg aatacgttac agattgaatc tgtgtttccg acgaattcta aaggaaaagt
     1561 tctggtggca tgttatcaaa aataaacgca accagtcaga aaacggctga ttgcgttttt
     1621 gttcaagtga tcccggcggg attcaaaccc acagccttct gatccgtagt cagatgctct
     1681 attcagttaa gctacgggac catttatcaa cagaatgcaa atataataaa atttgaaaaa
     1741 ataaagccaa tgcaggatgg aaaggaccgg aatgaaccgg aatgaaccgg attatctcag
     1801 atccggtcag gagagtcact ggtttaaggt tttaaagttg caataatttc attaatttta
     1861 cttcagtaaa tcccggatgt gccggttgca tacatttctg tcaatgttaa aaagatgatt
     1921 tccatatgtt tcatgtctgg cagcactgaa taaatacata agcaggcaaa ccgtcatatc
     1981 attaaaccaa aattttacaa tgatgaaaaa gcctttgaaa attctattgt tatccctttc
     2041 cgggctgatc cttcttgttt tcatcctgct tgtcagcgta ccggtgatct tcagggatga
     2101 aataaaggca aaagtggaac agattattaa tgaatccgta tatgccacag ttaatgtaca
     2161 ggactacagg ctgaattttt tcaggaattt tccgaatctt gccctgggac tggacaatgt
     2221 ttcagtggtg ggcataggga aatttgaaaa tgacaccctt gcagcattca ggtcactgga
     2281 tctcgttttt taccttccca gtgtatttaa aaagacagga tacgagatca gatcaatcgt
     2341 gattgacaaa gccctgctga ctgttaccta tctggatgac gggtcattta actgggacat
     2401 atcacgggat acagcctcag ttgcaacaga tgcggctgat gcagaatctt cagatatgaa
     2461 aatcctgctg aaaaaggtaa aacttgtaaa cagcaccctt gtctataatg attatggact
     2521 tgacatgaag gcgcttataa atgatctgag ttttttactc tcgggtgata tgaccgcatc
     2581 ggaaacagat ctgaagataa aacttactgc cggggatgtg aattttataa tggacggaat
     2641 aaaatatctg aacggggcca gggccaaagg agagcttgac ttgtcagcaa acctcgatga
     2701 ttataaattt acgttccgcg aaaatcatct gaccataaat gatttgaaga tcaatttttc
     2761 cggatgggtt gcaatgcccg aagatgatat tgaaaccgac ctcaccttta aatctgagca
     2821 gacctcattc aaaacacttc tgtctctcat tccggctgtt tatatgcatg attaccgcga
     2881 tctgaaggct gcgggaaatt tttcattttc cggatcagcc agagggatct acagcgatgc
     2941 tgattcaaca atgcctgata ttgatctcag gcttgttgtt aatgacggac ttgtgagcta
     3001 tcctgcactt cctgaaaaaa taagcagtat tgatcttaag gctgaagctt tcgttgacgg
     3061 gaaagatatg gacaatacag tggtgaatat cgaaaaattc catatggaac tggcaggtaa
     3121 cccctttgat ttcggatttg cccttaaaac acctttgagt gaccctgata taaaactttc
     3181 aatgaacggg aaactggatc ttgatgccct ttcgaatgca attcctcttg acagcatgga
     3241 tctctcagga cttgttgatg tatcgctgat gcttgcagga cagctttcca tgattgaccg
     3301 gcagcagtat gacgacttcc gggcttcagg aaaattgtca gtaaaaaata tgcgggtgga
     3361 aatgacaggt tatccggaaa tcagggttaa taatgccgga tttgaattta caccggctta
     3421 tgcagctctt ccgggtgcag atcttctgat tggtgaaaac agcgatttca gcctcagcgg
     3481 aagactggaa aactatatcc cgtatatt
//
LOCUS       DAOSPX010000333         3472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_24367_length_3472_cov_3.284167, whole genome shotgun sequence.
ACCESSION   DAOSPX010000333 DAOSPX010000000
VERSION     DAOSPX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24367_length_3472_cov_3.284167"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="PLZ75_12670"
     CDS             <1..1458
                     /locus_tag="PLZ75_12670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOS73289.1"
                     /translation="PVASVDLMERAALKLLEWYRRKFTRSRRILIFAGPGNNGGDGLA
                     LARLLEEERYEPELFYVHFSDTTSQDWKINRERLQKSTRVPFRIIDSAEQFPLTGPDD
                     VIIDAIFGSGLSRPVTGLPSEVIRMINQSGRTVIAIDIPSGLMGEDNTSNDYDSIVRA
                     DYTLSFQFPKLSFLFRENEIFVGEWVVLPIGLSPGAIAAINTPWELVEKAHVASLLKK
                     RRKFDHKGIYGHALLIGGSAGKMGALVLAARAALRTGAGLVSCHVPAGGGVIMQCSVP
                     EAMVISDRENDYISGIDPTEAFDAFGIGPGMGTRKESGEALGRFLLSCRNPVVIDADA
                     LNLLSVNKEYLDCLPEKTILTPHPKEFERLAGRAASDFQRLEKQVEFSVRYKCVVVLK
                     GAFTSVTDSSGNVWFNSSGNPGMATAGSGDVLTGMILSLLAQGYDTVNAALAGVCLHG
                     LAGDVAAEKLSFEAIIASDIVESISGAYRRIREEI"
     gene            1572..2660
                     /locus_tag="PLZ75_12675"
     CDS             1572..2660
                     /locus_tag="PLZ75_12675"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOS73290.1"
                     /translation="MIILLAGSCSLFRNTSKSYTRVTPLSDTTVLREGSLIYALPRTM
                     FTIKVELERTIELPGPYAGYADELLGLRNVILHENENWMIRSVSVSSHEEADPSEFYV
                     IESNTQMQSNYLALRKEGLIFDLYPRTSARLIDESDGKEIDVSRFRSFDLGSDEYYRV
                     QTDTVYKRVSVDEQFIRIPYIVEKRKKLSDAQLAEKAARRLMDLRDGKFMILMGEANV
                     FPQSTAAIDEINRMERELTELFTGKTITDIRTFTYSLVPEKRMEGAEVGIFRFSELTG
                     PEAVDSESGMPVVIEMVPERKTKDIAVISRGPADSSPSGPSRVYYRIPDIVNVKIRLG
                     DETLYNSRKLVYQFGTVMQLPANYIIGR"
     gene            complement(2661..3206)
                     /locus_tag="PLZ75_12680"
     CDS             complement(2661..3206)
                     /locus_tag="PLZ75_12680"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOS73291.1"
                     /translation="MRKIFTLLLLTVVTSGLYSQVASPSAGTGVRWYTMEEAEKLYNK
                     APRPIFIDTYTDWCGWCKKMDNETFTDPVIADLLNRKFYPVKFNAEGKNSVTFMGQTF
                     INDGKAGRAHQLAVALLQGQLSYPTVVFMTREDDRFAVSPVPGFRAPRDMEVLLSFFA
                     DDAYKTQKWDEFQKNFTGRLQ"
BASE COUNT          940 a          737 c          853 g          942 t
ORIGIN      
        1 cctgttgctt ctgttgatct tatggaacgt gccgctctga aactccttga gtggtacagg
       61 agaaaattta cacgttcacg cagaatcctg attttcgccg gacccggcaa caacggcggt
      121 gacggactgg cactggcaag attgctcgaa gaagaaagat atgagccgga actcttttat
      181 gttcatttca gtgatactac ttctcaggac tggaagatta acagggaaag acttcagaaa
      241 tccacgcgtg ttccgttcag aattattgat tctgcagaac aatttcccct gacaggcccg
      301 gatgatgtaa ttattgatgc aattttcggc tcggggctgt cccgcccggt aacagggctc
      361 ccctcagaag taataaggat gataaatcag agcggaagaa ccgttattgc cattgatatc
      421 ccttccggac tgatggggga ggataatacg tcaaatgatt atgacagtat tgtaagagcc
      481 gattataccc tcagtttcca gtttcccaaa ctgtcattcc tgttcaggga aaacgaaatt
      541 ttcgtggggg aatgggtcgt tctgcccatt ggtctttccc ccggggccat agctgctata
      601 aatactccgt gggagctagt ggaaaaagcg catgtggctt cccttttaaa gaaaaggcga
      661 aagtttgatc ataaaggcat atacggacat gctcttctga taggaggttc tgcgggaaaa
      721 atgggagcac ttgtactggc tgccagagca gcgctgcgta caggtgccgg actggtaagc
      781 tgccatgtcc cggccggcgg gggcgtgatt atgcagtgca gcgtaccgga agcaatggtc
      841 atttctgaca gggagaatga ctatatttcc ggtattgatc ccacggaagc ctttgatgct
      901 ttcgggatag ggcccggaat ggggaccaga aaagagtcag gcgaggctct cggcagattc
      961 ctgctttcat gcagaaatcc cgttgtaatt gatgctgatg ccctgaacct cttgtctgtg
     1021 aacaaggaat atcttgactg tcttccggag aaaacaatac tcacaccgca tccgaaagag
     1081 tttgaaagac ttgcaggcag ggcagcatca gatttccagc gccttgaaaa acaggttgaa
     1141 ttttcagtga ggtataaatg tgttgtggtg cttaagggtg cctttacttc agtcactgat
     1201 tcctcgggca atgtctggtt caacagcagc ggcaatcccg ggatggcaac ggcaggaagc
     1261 ggagacgtcc ttaccggaat gatcctttct cttctggccc agggatacga cacggtgaat
     1321 gcggcactgg ccggggtctg ccttcatggc cttgccggcg atgttgccgc ggaaaaattg
     1381 tcctttgaag ccattattgc ttctgatata gttgagagta tttccggagc ctacaggaga
     1441 ataagggaag agatctgacg ttttaagaaa caaatgctgc aaagttatat cttagcagga
     1501 tgagcttttt aattattgaa tttttaaaaa atatactgat gaaaaaaaca ggactattac
     1561 ttacttattt aatgataata ttacttgcag gttcgtgctc ccttttccgg aatacatcga
     1621 aatcctatac aagggtgaca ccgctttcag atacaacggt gctgagggaa ggcagcctga
     1681 tttatgccct gcccagaacc atgtttacca taaaagttga acttgaaaga acaatcgagc
     1741 ttcccggtcc ctatgcagga tatgctgatg agctgcttgg cctcaggaat gtcatcctgc
     1801 atgaaaatga gaactggatg ataagaagtg tttcagtcag ttcacatgaa gaagctgatc
     1861 cttcggaatt ctatgtaatc gaaagtaata cgcagatgca gtcgaattat cttgcactca
     1921 ggaaggaagg actgatattt gatctgtatc cccggacatc tgcccgctta attgatgaat
     1981 cagacggtaa ggaaattgat gtcagccgtt tcagatcatt tgacctgggc tcggatgagt
     2041 attacagggt tcagaccgac actgtctaca aacgggtaag tgtggacgag cagtttatca
     2101 ggattccgta cattgtggag aaaaggaaaa aactgtctga tgcccagctt gccgaaaagg
     2161 ctgcaagacg gctgatggat ctcagggacg ggaaattcat gattcttatg ggtgaggcaa
     2221 atgtctttcc ccagagcact gcagccattg atgaaattaa ccgtatggag agggagctta
     2281 ctgaactgtt tacaggcaaa acaattacgg atatcagaac atttacctac agccttgtgc
     2341 ctgaaaaaag gatggagggt gcagaggtgg ggatattccg cttttcagaa ctgacaggtc
     2401 cggaagcagt tgactctgaa tccggcatgc ccgtagtcat agagatggtt ccggaaagaa
     2461 aaacaaagga tattgcagtt atttcccgcg gtcctgctga ttcatctcct tccggcccct
     2521 ccagggttta ttacaggata cccgatattg taaatgtaaa gataaggctg ggagacgaaa
     2581 cattatataa ttcacggaag ctggtatacc agttcggcac agttatgcaa ttgccggcaa
     2641 attatataat tggcaggtga ttactgaagc ctgcctgtga aatttttctg aaactcatcc
     2701 cacttctggg ttttgtaggc atcatctgca aagaaactca gcaatacttc catatccctg
     2761 ggtgccctga accctggtac cggggaaact gcaaagcggt catcctccct ggtcatgaaa
     2821 accacggtgg gatatgaaag ctgtccctga agaagggcca ctgcaagctg atgagccctt
     2881 ccggctttac cgtcattgat gaaggtttgt cccatgaatg taacactgtt ttttccttct
     2941 gcattgaatt tcaccggata gaatttcctg ttcagcagat cggcaatgac gggatcggta
     3001 aatgtttcat tatccatttt tttgcaccat ccgcaccagt cggtataggt atcaatgaat
     3061 ataggacgcg gggctttatt atataatttt tccgcttctt ccattgtgta ccatctgaca
     3121 cctgttccgg ccgaagggga tgcaacctgg gaatacagac ccgatgtgac tactgttaac
     3181 agcaatagtg tgaatatttt tctcattttt ctgtgttaat taatgcaaaa taacttcatt
     3241 ttcctgaatt gataaaatca ttcagacatt tccgccatga aatgacaaca attccgggca
     3301 ggtaatgttc attgcagatt aatatcagga gattcatccg tttatttttt tacatttacc
     3361 gccattaagc tctggggatt tatggaaaca aatttaagaa cactgcgtga ttcagccgct
     3421 ttaaggtggc tcgtcctggt tttgctgagt gcactgatgt ttgccacata ct
//
LOCUS       DAOSPX010000334         3469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_24392_length_3469_cov_4.197715, whole genome shotgun sequence.
ACCESSION   DAOSPX010000334 DAOSPX010000000
VERSION     DAOSPX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24392_length_3469_cov_4.197715"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLZ75_12685"
     CDS             <1..519
                     /locus_tag="PLZ75_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS73292.1"
                     /translation="KSLFSAGTTWPGVFLAEDLKALDILCSRDDVDSSRIGCGGLSGG
                     GMRTVFAGGFDERIRCAVCVGFMTTWKDFILNKSFTHTWMTYVPILPKELDFPEILGL
                     RVPLPTLVLNDSEDGLYTIGEMRKADSILREIFEKAGAASNYHCSFHPGPHKFDIAMQ
                     NEAFDWFDKWLK"
     gene            543..1613
                     /locus_tag="PLZ75_12690"
     CDS             543..1613
                     /locus_tag="PLZ75_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOS73293.1"
                     /translation="MRKILRHSLALFLLSGLIISLAGQIKSPAADLLVHRNRSGRIKT
                     IRSERQWEKQRKNILENMQDVMGPLPAIAAKVSPEPEITEEFSKDGIRFRKILFASEK
                     DDRVPAYLLIPEDISGPVPGILCLHQTIVAGKAEPAGLAGNPNLHYALELARRGYVTL
                     APDYPNFGDYEYDPYRNGYQSATMKGIWNHMAAVDLLQSLPEVDPGRIGCIGHSLGGH
                     NTLFLAAFDKRIRAAVTSCGFNSFRKYYGGDLSGWSHKGYMPRIATVYDKNPDRMPFD
                     FPEVLAAISPRAVFISAPLHDSNFEVSGVWDCVNAAMPVYELLNASGNIIMINPDAPH
                     DFPDEARQAAYEFLDEKLKNGS"
     gene            1780..>3469
                     /locus_tag="PLZ75_12695"
     CDS             1780..>3469
                     /locus_tag="PLZ75_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73294.1"
                     /translation="MIINLLLICLYLLSLTGGCTSDHGKPVVIGNDHFSWEISREGRN
                     LHFTDRHSNIDWLSDDSASFCASVVSNGVTHHISSLESKGKRIIMTFGSAGIQAEIKI
                     TGKKRDWIGMRVEKITGEAESLNFINIPLRLEAMPDEQFAACVLSMNLFTHVEQLPAL
                     QSHLRAECHSRFGMEGAEISVLGVPMDRILPVIREIMRDAKDIPFSDKGGAFAQLEKE
                     GYGSYLMDFGSLTEESVDEIIDQCNSLGFNQVTLHGGNFFRHGEFEPERSKWPDGWKS
                     LKRINDRLHEAGISSILLTYTFFIDKSSVHVSPSASEDLDYFSSFTLAEPIDRDDTEI
                     VVRESTAGISPVTGFFVRNSNSLRINGEIIEFTGVSSSPPWKFTGCRRGACNTTVSAH
                     EAGEKAYHLKEMFGLFIPGPETGLFSHIAQRTAEMVDSCGFDGIYFDAIDGNDLLGGR
                     ENSWYYGTKFVFEVARHLKKPVGMEMCDMPHHYWHYSSRRQAWDRPVRGYKRFIDIHL
                     AAIKSDDHRHGEWMGHSPEINRLAAAENGKLLLPLHLGWWANYTWNPPQVEPTFP"
BASE COUNT          970 a          796 c          868 g          835 t
ORIGIN      
        1 aaatccctgt tcagcgccgg cacaacatgg ccgggtgtct tccttgccga agatctgaaa
       61 gctctcgata tattgtgctc aagagacgat gttgacagca gccgtatagg atgcggcgga
      121 ctgtcaggcg gaggaatgag aaccgttttt gccggaggtt ttgatgaaag gatcagatgt
      181 gcagtatgtg tgggattcat gacaacctgg aaggatttca tacttaacaa atccttcact
      241 catacatgga tgacctatgt tccgatattg ccgaaagaac ttgacttccc tgagatactg
      301 ggactgaggg tgcctcttcc cacccttgtt ctgaatgaca gtgaagacgg attgtatacc
      361 attggagaaa tgaggaaagc tgattcgatt ctccgcgaaa tattcgaaaa ggccggtgct
      421 gcatccaatt atcattgctc attccatccc ggaccccata aatttgatat agcgatgcag
      481 aatgaggctt ttgactggtt cgataaatgg cttaaataac tactgcgttt ttccataagg
      541 aaatgagaaa gattctcagg cattcactgg ccctgttcct tctttcaggc ctgataatat
      601 ccttagcggg acaaattaaa tctcctgcgg ccgaccttct ggttcacaga aaccgttcag
      661 gcaggattaa aaccatcagg tcggaacggc agtgggaaaa gcagcggaag aatatacttg
      721 aaaatatgca ggatgtaatg ggtccattgc ccgccattgc ggcaaaagtg tcaccggagc
      781 cggaaatcac agaagaattc agtaaagatg gaataagatt caggaaaatc ctttttgctt
      841 cagaaaaaga tgaccgggtt ccggcatatt tactgattcc cgaagatatt tccggacctg
      901 tccccggcat cctttgcctg caccagacaa tcgttgcggg taaagccgaa cctgcaggcc
      961 ttgctggcaa tccaaacctt cactatgccc ttgaactggc cagacgcgga tatgtgaccc
     1021 ttgcacccga ctatcccaat ttcggggatt atgaatatga tccttaccgt aatggctatc
     1081 aaagcgccac catgaaaggg atatggaacc atatggctgc agtggatctt ctgcagtcgc
     1141 tgcctgaagt tgatcccggc cggataggtt gtatcgggca ttcactggga ggacacaata
     1201 ctctttttct tgccgcattc gacaaacgta tccgggcagc agtcaccagc tgcggtttca
     1261 acagtttccg caaatattac ggtggcgacc tcagcggatg gagccataag ggatacatgc
     1321 ccagaatagc aacggtctat gacaaaaatc ccgacaggat gccttttgat tttccggagg
     1381 tcctggcagc aatatcccca cgggctgtat ttatcagtgc ccctctccac gacagtaatt
     1441 ttgaggtatc aggagtctgg gactgtgtta atgctgcaat gcctgtatat gaactgctga
     1501 atgcatcagg gaatattatc atgatcaatc cggatgcccc gcatgatttc cctgatgagg
     1561 caagacaggc tgcctatgaa tttctggatg aaaaattaaa aaacggatca tagaatgacc
     1621 ctgcaccgga taatgacccg gaaccagaga atgaccattc accggataat gacccggaac
     1681 cagagaatga ccctgcaccg gatgatggct cgcagccaga aaccggaccc gtaccggtat
     1741 ataaaccggt gtagaaaaac gaccctgcat caggggaaaa tgataataaa tttactgcta
     1801 atatgtctgt acctgctttc cctgacggga ggatgcacct cagaccacgg aaagccggtt
     1861 gtcatcggga atgatcattt cagctgggaa ataagtcggg aaggcaggaa tctgcatttt
     1921 accgacaggc acagcaatat tgactggctt tccgatgatt ctgcatcatt ctgtgcttcg
     1981 gttgtcagca atggtgtaac acatcatatc agctcccttg aatccaaagg aaaacgtatc
     2041 attatgactt tcggatcggc cggcatacag gccgagataa agattacagg aaagaaaagg
     2101 gactggatag gaatgcgtgt tgaaaaaata acaggggaag cagaatcact caacttcatc
     2161 aatattccat taaggctgga ggccatgcct gatgaacagt ttgcagcctg cgtgctttca
     2221 atgaatcttt tcactcatgt tgagcagctg cctgccctgc agagtcatct ccgggcagaa
     2281 tgccattccc gctttggcat ggaaggagct gagatatcag tgctgggcgt tccaatggac
     2341 aggatattac ccgtaataag agaaataatg agggatgcga aggatatacc tttctcggat
     2401 aagggagggg ctttcgcaca gctggagaaa gaaggatacg gatcctatct gatggacttc
     2461 ggaagtctta cagaagaaag cgttgacgaa ataatcgatc aatgcaacag tcttggcttc
     2521 aaccaggtga ccttgcacgg aggaaatttt ttcaggcacg gcgaatttga acctgagcgt
     2581 tccaaatggc cggatgggtg gaaaagtctt aaaaggataa acgacaggct gcacgaagca
     2641 ggaatatcat ctattcttct tacatacact ttctttatag ataaaagctc ggtacatgtt
     2701 agtccttctg cgagtgagga cctggattat ttcagctcct tcacactggc tgaacccatt
     2761 gaccgtgatg acactgaaat agttgtcagg gaatcaactg ccggcatatc cccggtaacg
     2821 gggttttttg tccgcaacag caattcactg agaataaacg gtgagataat agaatttacg
     2881 ggagtaagct cctcaccgcc ctggaaattc acaggatgca ggcgcggagc ctgtaacaca
     2941 accgtgtctg ctcatgaggc aggagaaaag gcatatcatc tgaaagaaat gtttggcctg
     3001 ttcattcccg gacccgaaac cgggttgttc agccatatag cacagcggac agccgaaatg
     3061 gttgattcgt gtggttttga cggaatctat tttgacgcca tcgacggcaa tgaccttctc
     3121 ggaggcaggg aaaattcctg gtattacgga acaaaattcg tttttgaagt agcccgacac
     3181 ctgaaaaagc cggtgggaat ggaaatgtgc gacatgcctc accattactg gcattacagc
     3241 tcccgcaggc aggcatggga ccggccggta aggggatata aaaggtttat tgatattcac
     3301 ctggctgcaa ttaaatcgga tgatcaccgt cacggggaat ggatgggcca tagtccggaa
     3361 ataaacaggc tggcagctgc tgaaaacggc aaactgcttc taccccttca tctgggctgg
     3421 tgggccaatt acacctggaa tcccccgcag gttgaaccca ccttccctg
//
LOCUS       DAOSPX010000335         3465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_24435_length_3465_cov_4.020821, whole genome shotgun sequence.
ACCESSION   DAOSPX010000335 DAOSPX010000000
VERSION     DAOSPX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24435_length_3465_cov_4.020821"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..3300
                     /locus_tag="PLZ75_12700"
     CDS             100..3300
                     /locus_tag="PLZ75_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS73295.1"
                     /translation="MKLMTLLLLLGMSQLTVLADRNGDGSTHNLQQMAVTGRVIDVAG
                     NPVAGVNIIEKGTTNGAISNADGVYSITVASPNSVLSFSFIGYVTQEITVGTQTVINL
                     TLAESLSALDEIVVVGYSTQVRRSLTGAISTVSSEALTTATTANVITRLQGQASGVNI
                     INAKAPGGGATVTIRGLGTINNNTPLYVIDGVPTKSGITELNPEEIESITVLKDASSA
                     AIYGARGANGVILITTKHGMAGRTKITMNARFGISGFSKYYDLLNTQEYGEMLWLEAE
                     NMGVPPNHPLYGSGKDPKIPDYVFPAGKMEGDPLVNPSRYSHTPGATFYNITKANKEG
                     TKWFDEIRQPGIMNEYNVSLTGGSDKGTHAFTIGYFTERGIMKYTSFDRYTLRSNADS
                     KITKWLTIGESLGLSYAVGKGNRNEYGEAIPISQSYRMQPIIPVYDIMGNFAGTKGTG
                     TGNGENPLAYLWRDKDDYSHNLRGIGNVYGEIQVIEGLRLKSLFGFDYRPYDSRDIFR
                     QNPEFQEAKPSDILYMYSGYGTQWNWTNTLNYVKTLAGLHTLNFLAGTEAVAVESKDI
                     SASRSTYFSDDPIYMYLNAGEKDQTNGGSGSEVRWMSYFGRLNYDYAGKYLAELTFRR
                     DGSSRFGENSRWGNFPAFSAGWTLSEEGFMAGTRDYIDNLKIRAGWGMLGNDEVGNYN
                     GFSTFGSNSGYSYYSINGAVSSLASGFYASAKGNPDAKWETTITTNVGIDVTALNNTL
                     SMTLDIWERKTKDMLFRVAIPHVVGAATAPSVNIGDMDNKGFDLNLNYQNSAMGGDLT
                     YNAGLTLSHYKNKIVKISGKEAEFISGGDFRQMVYTRATTGTSYPEFYGLIVDGYLQT
                     QEEADAYPPMYGGVYNIAGHFKYRDVNKDGKVNDDDRGYIGSPHPKFTAGLNANVGYK
                     GLALNAFFYSSYGNKLCNYVRRWIDFTNFTGNRTKDRLYRSWGSPYLKDNKDAVLPLA
                     DETTVSQYPSTFFLEDGSFLRLKTLQLSYTLPKSVSQRLTLNNLELYIQGTNLFTLTK
                     YSGLDPEVSRAGVNLGVDDGQWPTARQFVFGIRLDI"
BASE COUNT         1025 a          787 c          792 g          861 t
ORIGIN      
        1 ttaaagaaca aaagcaccct tttttttaag tacttatgaa taattcaggt tagaacgaat
       61 gtttcatttc tctggctttt tttcaggaaa aaaccggtaa tgaaactgat gacccttctg
      121 ctgttgctgg ggatgtctca gcttacggtc cttgcagacc ggaatggaga tggaagtacc
      181 cacaacctgc agcaaatggc tgttaccgga cgggtaattg atgttgcggg caaccctgtt
      241 gccggagtca atatcatcga aaagggaaca acaaacggtg caatttcaaa tgccgacggg
      301 gtatattcaa ttacggttgc ttctccaaat tccgttttga gtttttcctt tatcggttat
      361 gtaacccagg agattaccgt gggaacgcag actgttatca accttacgct cgccgaaagt
      421 ctgtccgcac tggatgagat agtagttgtc gggtattcaa cccaggtcag gcgatctctt
      481 accggtgcca tctccactgt cagctccgag gccctgacca cggcaaccac cgccaatgtc
      541 attacgcgtc ttcagggaca ggcttccggt gtaaatatta ttaatgcaaa agcaccggga
      601 ggcggtgcca cagttacgat caggggattg ggaacaatca ataataacac cccactgtac
      661 gttattgacg gggttccgac aaaatccgga ataacagaac tcaacccgga agaaatagaa
      721 tccataacag ttctgaaaga tgcttcctct gctgcgatat acggggcaag aggagcaaac
      781 ggagtgatac tgataacaac aaagcatggt atggcaggaa gaacaaaaat cacaatgaat
      841 gcccgtttcg ggatcagtgg attctcaaaa tactatgacc tgctcaatac ccaggaatat
      901 ggtgaaatgc tatggcttga ggcagaaaac atgggtgtgc ctccaaatca tcccctgtat
      961 ggatcgggaa aggatccaaa aataccagat tacgtattcc cggcaggtaa gatggaagga
     1021 gatccccttg tgaatccttc tcgttacagt cacactcctg gagcaacttt ctacaacatc
     1081 acaaaggcaa acaaggaagg caccaagtgg tttgatgaga tacggcagcc cggtatcatg
     1141 aatgaataca acgtctccct gaccggagga agcgacaagg gaacacacgc ctttacaata
     1201 ggctatttta ccgaaagagg tattatgaag tataccagct tcgaccgcta tacccttcgt
     1261 tcaaatgctg acagcaaaat cacaaaatgg ctcaccatag gagaatccct tggcctttcc
     1321 tatgccgtcg gcaagggtaa ccggaacgaa tatggtgaag caattccgat ttcacagtcc
     1381 taccgcatgc agcccataat tccggtttat gatattatgg gtaattttgc cggtacaaaa
     1441 ggaaccggaa caggtaacgg tgaaaatccc ctcgcatatc tttggcgcga caaggatgac
     1501 tattcacata atctcagggg aataggaaat gtatatggtg aaattcaggt gatcgaaggt
     1561 ctgagactca aatcattgtt cggtttcgac tacagaccct atgacagccg ggatattttc
     1621 cgtcagaatc cggaattcca ggaagcaaaa ccatccgaca ttctttatat gtacagcggc
     1681 tacggcacac agtggaactg gacaaacaca cttaactatg tcaaaacctt agccggcctt
     1741 catacattga attttcttgc cggaactgaa gcagttgcag tcgaaagtaa agatatcagc
     1801 gccagtcgct caacttactt ttctgatgat cccatttaca tgtatctgaa tgcgggtgaa
     1861 aaagaccaga caaacggcgg atcaggaagt gaagtcagat ggatgtccta tttcggaagg
     1921 ttgaattatg attatgcagg caaatatctt gccgaactca ctttccgccg cgacggctca
     1981 tcaaggttcg gagaaaacag tcgctggggc aacttcccgg ccttctctgc cggatggaca
     2041 ctctcagagg aaggattcat ggcaggcacc agggattata ttgataacct gaaaatcaga
     2101 gcaggatggg gaatgctggg taatgatgag gtaggaaact ataacgggtt ctccacattc
     2161 ggttcaaaca gcggatattc ctactattca attaatggtg ccgtaagttc cttagcatct
     2221 ggattttatg cctctgcaaa aggtaacccg gatgcaaaat gggaaaccac tataaccacc
     2281 aacgtgggga ttgacgttac tgctctgaac aacaccctga gcatgactct cgacatatgg
     2341 gaaagaaaga caaaggatat gctcttccgt gttgcaatcc ctcatgtggt gggtgctgcc
     2401 acagctccat cagtgaacat cggtgatatg gacaacaaag gctttgacct gaacctgaac
     2461 tatcagaaca gtgcaatggg cggggacctt acctataatg caggcctgac cctgtcacat
     2521 tacaagaaca aaatcgtcaa aatctcagga aaggaagctg aatttataag cggcggagat
     2581 ttcaggcaga tggtttatac ccgggcaaca acaggtactt cctatcctga attttacggt
     2641 ctgatcgttg acggttacct tcagacgcag gaagaagcgg atgcttatcc tccaatgtat
     2701 ggcggagttt acaatattgc gggtcatttc aaataccgtg atgtaaacaa ggacgggaaa
     2761 gtcaatgatg atgaccgtgg ctatataggc agcccgcatc cgaaatttac tgcaggactt
     2821 aatgccaatg taggatacaa gggtcttgca ctgaatgcct tcttctattc aagttacgga
     2881 aataaactct gcaactatgt ccggcgctgg atagacttta caaactttac aggtaaccgt
     2941 accaaggacc gcctttacag atcatggggc agcccctacc tgaaggataa taaagatgct
     3001 gttctcccgc ttgccgatga aacaaccgtg agtcagtacc cctcaacttt cttcctcgaa
     3061 gacggttcct tcctcaggct gaaaaccctg cagctgtcct acacattgcc gaaatcagta
     3121 tcgcagaggt taaccctgaa taatcttgaa ctttatattc agggaaccaa tctctttact
     3181 cttacaaaat actccggact tgatcccgaa gtttcaagag ctggtgtaaa ccttggagtt
     3241 gatgacggac aatggcctac tgcccggcag tttgtatttg gcatcaggct tgatatataa
     3301 taagttcaat taatgaaaac agacaaaatt cagataatat gaaaaagaat attatatttt
     3361 caacactcgc tgtcggatta ctgattgcct ttctgatcgg atgctctgat gattttttga
     3421 atacacaacc gacatcttcc ctcagcgacg ttgttcttgc aaatg
//
LOCUS       DAOSPX010000336         3442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_24656_length_3442_cov_4.984942, whole genome shotgun sequence.
ACCESSION   DAOSPX010000336 DAOSPX010000000
VERSION     DAOSPX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24656_length_3442_cov_4.984942"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLZ75_12705"
     CDS             complement(<1..204)
                     /locus_tag="PLZ75_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73296.1"
                     /translation="MFSKLFRGTGPGVIFLIVVILALMWISAFIEPGTGEAAVYESDP
                     MPLYAVVKMITGPNVWSGLIFTML"
     gene            complement(208..1479)
                     /gene="purD"
                     /locus_tag="PLZ75_12710"
     CDS             complement(208..1479)
                     /gene="purD"
                     /locus_tag="PLZ75_12710"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOS73297.1"
                     /translation="MNILILGSGAREHVLAWKLTQSKKAGRIFAGPGNAGTAAAGTNL
                     AIDPCRFGQVRDAVLANDIGMVIAGPEAPLVAGIHDYFLSDPLLKNIPLIGPCRSAAR
                     LEGSKDFAKAFLMRHGIPTSRYRTFNRATAHEVPEFLATLRSPYVIKADGLAAGKGVL
                     IIDDINEAAAEVNAILDGKFDSAGEKVVVEQFLKGIELSAFILTDGKSWKLLPEAKDY
                     KRAGSGDTGLNTGGMGAVSPVPFAGKEFMDKVRDRIIEPTMRGLAEEGIVYTGFLFFG
                     LMNVDGDPYVIEYNVRMGDPEAEVVIPRIKSDLLDLLEGVAEGNLSERSLETDSRFVT
                     TVMLVSGGYPGSYKNGMEITGLEKTSDCAVFHAGTKAEGERLLTSGGRVLAVSSWGKS
                     LNEALEGSYSNAAQISFAGMYYRKDIGFDLK"
     gene            complement(1558..2169)
                     /locus_tag="PLZ75_12715"
     CDS             complement(1558..2169)
                     /locus_tag="PLZ75_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS73298.1"
                     /translation="MRFFTIPSLAARIYPEALFRIRTREKILCLTFDDGPDPESTPEL
                     LEILERHNVRSIFFCDGRAAETYPGLVGMIRDRGHIVGNHGYNHLNGWSTSAGEYCRD
                     VKRASGFTSASLFRPPYGLLRYNQYLRLKTSFRIVFWDVMPYDFDREFPPARSLEILN
                     RRIRPGSVIVLHDRQDSTSVLFLDEFLETSAGKGWEFRCPDLQ"
     gene            complement(2176..>3442)
                     /locus_tag="PLZ75_12720"
     CDS             complement(2176..>3442)
                     /locus_tag="PLZ75_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73299.1"
                     /translation="TAAGPVLMAAENWDDHDRSGRYTARDIGANYLESCAPGAVIFTY
                     GDNDSFPLWYIQDVEEVRTDVRVANLSYLQAGWYIEMMRQKAFKSEPLPLSLDQDKYR
                     EGQRTQIPVLSRIDDPVNIRELVNFAGMDDRKYLVDISGRGDYVNYFPGNKVLIDVDT
                     SVVLSNGTVKEYFRNRLLSPIVWEITSSDAFKNDLAIMDLLATSKWTRPVYFSTTVPS
                     SQYNGLEKFFVQEGMAYRIVPINTDNSQGGDYGIIDHVVMYDNMMNRFRWGNAEDPSV
                     YLDENNKRMFSNFRRMFGNLGRALLAAGDTVRAVEVARRGMEIVPAEKLQHDFFSIGL
                     AEVLIKAGQKEEGLRLAGDIINHAREYLDYAISLDAGRRYGLDYPTGINMQALLDLYR
                     LSIDLKESDLTSITEALIGNYYDRLYSRG"
BASE COUNT          833 a          973 c          752 g          884 t
ORIGIN      
        1 cagcattgtg aatatcagtc cggaccagac attcggaccg gttatcatct ttacaacagc
       61 atacagcggc atgggatcgg attcatatac agctgcctca cctgttccgg gctcaatgaa
      121 tgcactgatc cacatgagtg caaggatgac aacaatcagg aatatcactc ccggtccggt
      181 tcccctgaat aattttgaaa acatgtccta tttcaagtca aatcctatat ccttcctgta
      241 atacatgccg gcgaaggaaa tctgtgccgc attgctgtat gatccttcaa gggcctcatt
      301 caggctcttt ccccatgagc tcactgccag gaccctcccc ccggaggtca gcaggcgctc
      361 cccttccgct tttgttccgg catggaaaac ggcgcaatcg cttgtttttt ccagtcccgt
      421 tatctccatc ccatttttgt agcttcccgg gtatcctcct gatacaagca tgacggtagt
      481 aacgaagcgg ctgtcagttt caagggagcg ttccgacaga tttccttccg caacaccttc
      541 aagcagatcc aggagatccg actttatcct gggaataacc acctccgcct caggatcgcc
      601 catgcggaca ttgtattcaa tcacgtaggg atcgccgtca acattcatca gtccgaagaa
      661 caggaatccc gtgtatacaa tgccttcttc ggcaagcccc ctcatcgttg gttcaattat
      721 tctgtccctg accttgtcca tgaactcctt cccggcaaag ggcacaggcg acacagcacc
      781 cattcccccc gtattcagtc cggtatcccc gcttccggcc ctcttgtagt cctttgcttc
      841 aggaagcaat ttccaggatt tcccgtcagt aaggataaaa gccgataatt cgatgccctt
      901 cagaaattgt tccactacaa ccttttcccc ggcggaatcg aatttcccgt ccagtatggc
      961 atttacctca gcggccgcct cattaatgtc gtcgattatc agaacaccct tgcccgcagc
     1021 cagtccgtca gccttaataa cataaggact ccggagagtg gcaagaaatt caggtacctc
     1081 atgtgcagtc gccctgttga aagtccggta gcgcgatgtg ggtatgccgt gacgcatcag
     1141 aaatgccttt gcgaaatcct tgctgccttc aaggcgcgca gccgacctgc agggaccgat
     1201 aaggggaatg tttttcagca ggggatcgga aaggaaataa tcatgtatgc ccgcaaccag
     1261 cggtgcttcc gggcccgcta tcaccattcc tatatcgttt gcaagcactg catccctcac
     1321 ctgaccgaac ctgcagggat ctatcgccag gtttgttccg gctgcggctg taccagcatt
     1381 accgggaccg gcaaagatcc ttccagcttt tttgctctgt gtcagtttcc atgcaaggac
     1441 atgctccctt gcgcccgaac ccaggataag aatattcatc tgcgcattta atttggcttt
     1501 tcccgaaaat tataatttcc agctataaaa gtaaagcctg ctgagcaata attcaaatta
     1561 ttgtaagtca ggacagcgga attcccagcc tttcccggct gaagtctcaa gaaattcatc
     1621 aaggaacaga accgaagtcg aatcctgcct gtcatggagt actatcactg atcccggcct
     1681 gatccgcctg ttcaggattt caagtgaccg ggccggcgga aattcccggt caaaatcata
     1741 gggcatcaca tcccagaata ctatcctgaa tgaagtctta aggcgaagat actggttata
     1801 gcgtaataat ccgtacgggg gccggaataa acttgctgaa gtaaatccgg aagccctttt
     1861 cacatccctg cagtattcac ccgctgaggt actccagccg ttcaggtgat tatagccgtg
     1921 atttcccaca atatggcccc tgtcccttat catgcccacc aggcccggat aggtttcagc
     1981 cgctcttccg tcacagaaaa aaatggacct tacattatgc ctttccagta tttcaagaag
     2041 ttccggagtg gattccggat caggtccgtc atcaaatgta aggcaaagga ttttctccct
     2101 cgtcctgatc ctgaacaggg cttcgggata gatccgggca gcaagggagg ggatggtaaa
     2161 aaacctcata acctgtcagc ccctgctgta aagcctgtca tagtagttac ctatcagggc
     2221 ctcggtaatg cttgtcagat cgctttcctt cagatcaatt gacagccggt agagatccag
     2281 aagtgcctgc atgtttatgc ctgtcggata gtcaagtccg tatctcctgc cggcatccag
     2341 actgatggcg taatcgagat attcccgggc atgattgatg atatcccctg ccagcctcag
     2401 gccttcctcc ttttgtcccg cctttatcag tacttcagcc agtcctatgc tgaaaaagtc
     2461 gtgctgaagc ttttcggccg gaacaatctc catgcccctc cttgcaacct caacagccct
     2521 gaccgtatct cctgccgcca gcaatgccct tccaaggttt ccgaacattc tcctgaaatt
     2581 gctgaacata cgtttattgt tctcatccag gtaaaccgag ggatcttcag cattgcccca
     2641 tctgaacctg ttcatcatat tgtcgtacat gaccacgtgg tcaattattc cataatcccc
     2701 gccctgcgaa ttatccgtgt ttatcggaac tattctgtat gccatgccct cctggacaaa
     2761 gaatttttca agaccgttgt actggctgga aggcaccgtg gttgaaaaat aaaccggacg
     2821 ggtccatttg ctggttgcca gcaggtccat tattgcaaga tcattcttga atgcatccga
     2881 tgatgtaatt tcccatacaa tgggtgaaag aagcctgttc ctgaaatatt ccttaaccgt
     2941 tccgttggac agtacaacgg aagtatcaac atctataagc actttgttgc cggggaaata
     3001 attcacataa tcccctcttc cggagatgtc aacaagatat ttcctgtcat ccatccccgc
     3061 aaaattcaca agctccctga tattgaccgg atcatcaatc cttgacagta cagggatctg
     3121 tgtacgctgc ccctcacggt atttatcctg atccagtgac aggggaaggg gttccgactt
     3181 aaatgccttc tgcctcatca tctctatata ccatccggcc tggagatagc tcaggtttgc
     3241 caccctgacg tcggttctca cttcctcaac atcctgaata taccagagcg ggaaggaatc
     3301 attgtcgcca taggtgaaaa ttacagcacc gggagcacat gactccaggt aattggcacc
     3361 tatatccctg gctgtgtagc ggcccgaccg gtcgtgatca tcccagttct ccgcggccat
     3421 cagcacaggg cccgctgcag tg
//
LOCUS       DAOSPX010000337         3425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_24815_length_3425_cov_4.548368, whole genome shotgun sequence.
ACCESSION   DAOSPX010000337 DAOSPX010000000
VERSION     DAOSPX010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24815_length_3425_cov_4.548368"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLZ75_12725"
     CDS             <1..418
                     /locus_tag="PLZ75_12725"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOS73300.1"
                     /translation="HGGNDFKQMIRELGPLFPEMFIATCNWYQAVDQSRFFEAGGGHA
                     DEMETSLMLFLEPGLVLPVGNAGKGDAKKFRIKALNENWAWSERKWSRVTSDTGVGNP
                     SKATGEKGEKYFNAVTDSLCELIVDLARTDVNDMYV"
     gene            complement(415..957)
                     /gene="rsmD"
                     /locus_tag="PLZ75_12730"
     CDS             complement(415..957)
                     /gene="rsmD"
                     /locus_tag="PLZ75_12730"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOS73301.1"
                     /translation="MRIIGGKYRGRRIDLPTGFRIRPTTDFARESLFNILDNIIDFEE
                     TFVLDLFAGTGSIGYEFSSRGAAGVHLVEQNRKNVSGIKKIIGNIGFENIKVVHIDVR
                     TYLKICKAKYDVVFADPPYNLSWLEEIPALVTGSDILKPGGLLIIEHPAKVNFRNHEL
                     FFDHRNYGGVNFSFFRTKQA"
     gene            complement(945..1790)
                     /locus_tag="PLZ75_12735"
     CDS             complement(945..1790)
                     /locus_tag="PLZ75_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOS73302.1"
                     /translation="MPEQDLIDETFDINSSLNYELTMQISTAGFAYCILDTIRNRYVL
                     LRSRDPDENKAMTAEKTEEIIISDSFLSARFKKTNLILPSAKSTLVPSPLFDPLKKEE
                     YFSLNLVKDENEIILYNKITDPDAFVIFSVPEWLTDLTGRYFPSVKPCHHATSLIDQA
                     MHLSRDEAGQLVLVHLERDYFNLLVLERGSLKFYNTFVYKNMNDIMYYLFNVCKTLGV
                     KREENVWFSGLTSSHDELSSRVQEYLRNIKFMIPSGNFSFSYIFNSFEVHRYLSLFGI
                     SRCGL"
     gene            1934..3349
                     /locus_tag="PLZ75_12740"
     CDS             1934..3349
                     /locus_tag="PLZ75_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS73303.1"
                     /translation="MRDRIIYKSLCENLGNTPTEDQASALEKLASFITDGRTESIFLL
                     TGYAGTGKTSIISTVVRTLDNLRIRSVLLAPTGRAAKVLASHAGKQAFTIHKKIYRQR
                     SLKDGTGSFVLDRNLSRNTYFLVDESSMISNIAAESTVFGSGRLLDDLLEYVYSGTDC
                     RLILVGDDAQLPPVGAVISPALDRAVLEGYGFGLITAELRQVVRQSESSGVLMNATGI
                     RLQVSEGRLDHPSLDCRNYRDIVRISGEDLIEELSGSYGACGLEGTIVIVNSNRQANR
                     YNQGIRNRIFFREEEICAGDIVMIVRNNYSLMEEDESGPGFIANGDIAEIRRIRKYEE
                     RYGFRFAEMMLWFPDYDYELESLVLLDVLHLDSPSLPADRNKELFHSILEDYQHIRTK
                     GKQYTAVREDRYFNALQIKFAYAVTCHKAQGGQWERVFIDQGMFNRAEISLDYLRWFY
                     TALTRSTGKVYLVNFSDDFFI"
BASE COUNT          945 a          713 c          848 g          919 t
ORIGIN      
        1 ccatggagga aatgatttca aacagatgat cagggaactt ggtccgcttt ttccggaaat
       61 gtttatagcc acctgcaact ggtatcaggc agttgatcag tcacggttct tcgaggcagg
      121 tggcgggcac gcggacgaga tggaaaccag cctgatgctc tttcttgaac ccggactggt
      181 acttcctgta gggaatgccg ggaaggggga cgcaaaaaaa ttcagaataa aagcacttaa
      241 tgagaactgg gcatggtcgg agcgcaaatg gtcgagggtt acttcagata cgggtgtggg
      301 taatccgtcg aaggcaacag gggaaaaggg cgaaaaatat ttcaatgccg tgaccgacag
      361 cttgtgtgaa ctgattgttg atctcgcccg aaccgatgtc aatgatatgt atgtttaggc
      421 ctgtttcgtc ctgaaaaaac tgaaattaac ccctccgtaa tttctgtggt caaagaataa
      481 ttcatgattc ctgaaattta cttttgcagg atgttcgatg atcagcaggc ctcccggttt
      541 cagtatatcc gatccggtca caagggcggg aatttcttcg agccaggaca gattataagg
      601 cggatcggca aatacaacgt cgtattttgc tttacagatt ttcagatatg tacgtacatc
      661 aatatgaacg actttgatat tttcaaatcc aatattgccg atgatttttt ttattccgct
      721 gacattcttg cggttctgtt caacaagatg aactccggca gctccgcggg atgaaaactc
      781 atagcctata ctgccggtgc ctgcaaatag gtcaagaaca aatgtttcct caaagtcaat
      841 tatattatca agtatgttga acaggctttc acgtgcaaaa tccgtagtcg gcctgattct
      901 gaaaccggtt ggaagatcta ttctccttcc cctgtatttg cctcctataa tccgcacctg
      961 cttataccga aaagactgag ataccggtga acctcgaagc tattgaatat atagctgaag
     1021 gaaaaattgc ccgagggtat catgaatttt atattcctca gatattcctg aacccttgaa
     1081 gacagttcat catggctgga ggtgagtccg ctgaaccata cattttcctc ccgttttacg
     1141 cccagggttt tgcagacgtt gaacagataa tacattatat cgttcatgtt cttgtaaaca
     1201 aaggtattgt agaatttcag ggaaccccgc tcaaggacca ggagattgaa gtagtctctt
     1261 tcaaggtgaa ccagaaccag ctgacccgct tcgtccctgc tcaggtgcat agcctgatca
     1321 atcagggatg ttgcatggtg gcacggtttt actgagggaa aatacctgcc ggtcaggtct
     1381 gtaagccatt caggtactga aaagatgaca aaagcatccg ggtcagtgat tttgttgtac
     1441 aggataattt cattttcatc ctttaccagg ttcaggctga agtattcttc tttcttcagg
     1501 ggatcaaaaa gaggggaggg gacaagagtg gactttgctg aaggcaggat cagatttgtc
     1561 ttcttgaaac gggctgacag aaaagaatcg gaaataatta tctcttctgt tttttcagct
     1621 gtcatagcct tattctcatc aggatcccgc gaacggagca aaacatacct gttccttatg
     1681 gtgtcaagta tgcagtaagc aaatcctgca gtgcttatct gcatcgttaa ttcatagttt
     1741 aaggatgagt ttatatcgaa agtctcatca atcaggtcct gttcaggcat atttgttttt
     1801 aaattcttgt acagaatact taagtttacg gacaaaaata ggaaaaatac acaagctcaa
     1861 ccgtgctagt tcataaattg tgattcagga aaatatcgga cggcgggcag tataggcagc
     1921 cggacagaac aggatgcgtg acaggataat atataaaagt ctgtgtgaga atctggggaa
     1981 tactccaaca gaggatcagg cctcagctct tgaaaaactg gcttctttca tcactgacgg
     2041 caggacggag tcaatctttc ttcttacagg gtacgcgggt acgggtaaaa ccagtattat
     2101 cagcactgtt gtcagaaccc tggacaatct gagaatcaga tccgttcttc ttgcaccaac
     2161 aggcagggca gccaaagttc ttgcgtctca tgccggaaag caggctttta caattcacaa
     2221 gaagatatac cgtcagaggt cccttaagga cggtacgggc agttttgttc ttgacagaaa
     2281 tctgagccgc aatacatatt ttctagtgga tgaatcctcc atgatttcaa atattgctgc
     2341 agaaagtacg gttttcggca gcggcaggct cctggatgat ctgctggaat atgtttattc
     2401 aggaacggac tgcaggctca tccttgtagg ggacgatgcc cagcttcccc cagtaggtgc
     2461 ggttatcagc ccggcactgg accgggcggt tctggaagga tatggtttcg gcctcataac
     2521 ggcagagctc cggcaagtgg tccggcaaag cgaatcgtca ggcgtgctta tgaatgccac
     2581 aggcatcaga ttgcaggtga gtgagggcag actggatcat ccttccctgg actgcaggaa
     2641 ttacagggat attgtcagga taagcggcga ggatctgatt gaggaactat ccggctcata
     2701 cggagcatgc gggctggaag ggactatcgt gattgtcaat tccaacaggc aggccaacag
     2761 gtataaccag ggaataagaa acaggatttt tttcagggaa gaggaaatct gtgccgggga
     2821 tatcgttatg atagtcagga ataattacag tctgatggaa gaagatgagt caggtccggg
     2881 ttttattgca aacggggata tcgccgagat cagaagaata agaaaatatg aggaacgcta
     2941 tggtttccgc tttgctgaga tgatgctctg gttccctgat tatgattatg aacttgagtc
     3001 actggtattg ctggatgtgc ttcatcttga ttctccttca ctgcctgcag acaggaacaa
     3061 ggaactgttc cacagcattc tggaggatta ccagcatatc aggacaaaag ggaaacaata
     3121 tacggctgtg cgggaggacc ggtatttcaa tgccctacag atcaaattcg cctacgcggt
     3181 aacctgtcat aaggcccagg gaggccagtg ggagagagtg ttcattgacc agggcatgtt
     3241 caaccgggcc gaaataagcc ttgattatct gagatggttt tatacagctc tcaccaggtc
     3301 gaccggaaag gtatacctgg taaatttcag tgatgatttc ttcatctgaa ccctgaaaag
     3361 ggggttttat tcaccaatta ttttaacaag tactctcttt cttctctttc cgtcgaattc
     3421 accat
//
LOCUS       DAOSPX010000338         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_25154_length_3388_cov_3.443144, whole genome shotgun sequence.
ACCESSION   DAOSPX010000338 DAOSPX010000000
VERSION     DAOSPX010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25154_length_3388_cov_3.443144"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="PLZ75_12745"
     CDS             complement(<1..1946)
                     /locus_tag="PLZ75_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOS73304.1"
                     /translation="MYSGLSEFVTSLERENELIRIRAFADPVLEIAEIADRIVKSGGK
                     ALLFENNGTDFPLLINAFGSDRRMAMAMGRKSLDDAADETGRLFSELTRSRSSFREKI
                     KSLPLLLKLQAYMPSRARGRGLCQQVIHRDPDLGMLPVLKCWPCDGGRFLTLPVVHTV
                     HPLTGKTNAGMYRMQVLDKNSTAMHWQRHKTGASHFEEWKKTGRKMPVAVSLGGDPVY
                     TYAATAPLPENIDEFILASFLRGKKTRLVKCITNDLYVPADADIVIEGWIDPSEDPVW
                     EGPFGDHTGFYSLADWYPVFRVSCITHRKDAVYPATIVGIPPQEDAWLTKASEKIFLA
                     PLKFAIQPEIEDFHMPDAGVAHNLAIVKIRKTYPGQGKKVISALLGAGQMMFTKYLAV
                     VSGDVDIRNYTQLLGHVLDNTVFRRDLLFSQGPLDVLDHASDLPLLGGKLGIDATVKT
                     GEELTTDPSDEFLSQYNIPDGKGNNRQANAGNSSGSSPQVSYQHIRDEVLRKCRNACS
                     SFALLEGYPVLVAGTGQYDDPDALKKLKEVFSEPDMKSVFRLVLVVDDRLDVKDWFTI
                     VWQMLGNTDPGRGIIFLSDNSILADGTAKIFGKRAFKRKWPNIVCSSESTIRSVDMKW
                     DKLGLGPFIHSPSEKNSGMNFGEGA"
     gene            complement(2586..>3388)
                     /locus_tag="PLZ75_12750"
     CDS             complement(2586..>3388)
                     /locus_tag="PLZ75_12750"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOS73305.1"
                     /translation="FQEFMIRPVGAADFREGLRMGAEIFHSLKKVLRDRGLSTAVGDE
                     GGFAPTLDGTEDALETILKAVADAGYKPGQDIRIALDCAASEFYDKDRGIYDYSKFEG
                     PGGAKRTSKEQVDYLEQLIDKYPIDSIEDGMSEGDWDGWKLLTDRIGNRCQIVGDDLF
                     VTNVEYLRKGIETGCANSILIKLNQIGTLTETLDAIDMAHRAGYTTVISHRSGETEDS
                     TIADVAVAVNSGQIKTGSCSRTDRICKYNQLLRIEEMLGDLAVYGYKK"
BASE COUNT          774 a          925 c          762 g          927 t
ORIGIN      
        1 gccccttcgc caaaattcat tcctgagttt ttctccgacg gagaatggat gaaaggcccc
       61 agcccgagtt tgtcccactt catatcaacc gaccttatcg tgctttccga tgaacataca
      121 atattcggcc atttacgctt aaacgcacgt ttgccgaaaa tttttgccgt gccgtcagct
      181 agtatgctgt tatccgagag aaagataata ccccggccgg ggtcagtgtt tcccagcatc
      241 tgccatacta ttgtaaacca atccttcaca tcaagcctgt cgtcaacaac cagcaccagt
      301 ctgaaaacac ttttcatatc cggttccgaa aaaacttcct tcagcttctt cagggcatca
      361 ggatcatcat attgtcctgt tcctgcaacc agaaccggat atccttcaag caaggcgaaa
      421 gatgagcagg cattacggca cttcctgagg acttcatccc tgatgtgttg ataactgacc
      481 tgcggactgc ttccggagct gtttcccgca ttggcctgcc ggttgtttcc tttgccgtcc
      541 gggatattgt actgtgacag gaattcatca gaaggatctg tcgtcaattc ctccccggtt
      601 ttcactgtgg catcaattcc cagttttcct ccgagcaggg gcaggtctga agcatggtca
      661 agcacatcga ggggaccctg gctgaaaagg agatcccgcc tgaaaaccgt attgtccaga
      721 acatgcccca gcagttgcgt ataattcctt atgtccacat ctccgctgac cactgcaaga
      781 tacttggtaa acatcatctg ccccgccccg aggagcgcac tgatgacctt ctttccctgt
      841 ccgggatatg tttttcttat ttttacaatt gcaaggttgt gggcaactcc tgcatcaggc
      901 atatgaaaat cctcgatctc tggctgaatg gcaaacttca ggggtgcaag aaaaattttc
      961 tcgcttgcct ttgtgagcca tgcatcttcc tggggcggga tgcccactat tgttgcagga
     1021 tataccgcat ccttcctgtg tgtgatgcag ctgacgcgga atacgggata ccagtctgcc
     1081 agggaataaa aacccgtatg gtccccgaaa ggaccttccc acacgggatc ctccgacggg
     1141 tcaatccagc cttcaatcac aatgtccgca tctgccggca cgtacaggtc attggtaata
     1201 cattttacaa gccttgtttt tttccccctc aggaaggaag ccagtatgaa ttcatcaatg
     1261 ttttccggaa ggggagccgt ggctgcgtat gtatataccg ggtctccccc cagacttacc
     1321 gcaacaggca ttttccttcc tgtctttttc cattcctcaa aatgggaagc tcccgttttg
     1381 tgcctctgcc agtgcatggc agtactgttt ttatcaagta cctgcatccg gtacatacct
     1441 gcattggttt tccctgtcag tggatgaacg gtgtggacca ccggaagtgt gaggaagcgt
     1501 ccgccgtcac agggccagca tttcagtaca ggcagcattc caaggtcggg gtcccggtga
     1561 ataacctgct gacacaagcc cctgcccctt gcccgcgaag gcatatatgc ctgcagtttc
     1621 aataaaagcg gcagggattt tattttttcc cggaaggatg atctgcttcg ggtcagttca
     1681 ctgaacagac ggcctgtttc atcagcagca tcatccaggc ttttcctgcc catagccatt
     1741 gccattctcc tgtctgagcc aaaggcattg ataagcaaag gaaaatccgt tccgttattt
     1801 tcaaacagga gggctttccc tccggatttg acaatacggt cggcaatttc agcgatctcc
     1861 agaacgggat cggcaaaagc cctgattctt attaattcat tctctctttc aagagaagtg
     1921 acaaattctg ataatcctga atacatggga atttaaaatg ggataaagat acgggaatta
     1981 tagctgatcc cttaaaaaca gtaatgggaa ctattcacgt ggattagttc ccattcaccg
     2041 tttgtgaccg tggccaccta cggcgccgga ttactgttat tatggccggc tgcatggcag
     2101 gtcttgcgat ctgtgtctgc aacccgtggc tttcgggttt tgctttttac ggcagaacct
     2161 tccggctgtc cctttgttcc tgtcttccgg caggactttc cgggacggtg cgtcattcag
     2221 gccccggtga tgttcccgga ggaacggtac cggtttcagc tggctatggc cggagccata
     2281 tcttcacttc agccctgtga cccaatcgtt gaatgaacgt ttccgttttt cagcgatctg
     2341 ccgggacacc cggaactttc gtcccttcgc ccggaccccg gctctcctga tgtgcaggcc
     2401 cggaagcctg accgacccgg atccggcttc agttaagaaa ccatttccgg ctccacatct
     2461 tttgagcagc cttttatcgc tcatccgata gtgtaaatat aatacggaaa ccgtaaaaat
     2521 acagcaggaa aagcttgttg ttgtaaaccc gatatcaaca agcgttatca acaattgttg
     2581 ataacttatt ttttgtagcc gtaaactgcc aggtcgccaa gcatttcctc aatcctcagc
     2641 aactggttgt acttgcagat cctgtcggtc ctgctgcatg atccggtctt gatctgtccg
     2701 gaattaactg caacggcaac atcggcaatg gttgaatcct ctgtttctcc cgaacgatgg
     2761 gaaataaccg tggtatagcc tgccctgtga gccatgtcga tcgcatcaag ggtttcggta
     2821 agggttccta tctggttcag tttgatcagg atggaattgg cacaacctgt ttcaatccct
     2881 tttctgagat actcaacatt tgtaacaaag aggtcatcgc cgacaatctg gcatctgttt
     2941 cctatcctgt cagtcagcag cttccatccg tcccagtctc cttccgacat tccgtcctca
     3001 atggaatcaa tgggatactt gtctataagc tgttccagat agtcaacctg ttcctttgaa
     3061 gtccgttttg cacccccggg tccttcaaac ttcgaataat catatatgcc cctgtcctta
     3121 tcatagaatt cggatgcagc gcagtccagt gctatcctga tatcctggcc gggcttgtat
     3181 cctgcatcag caacagcctt cagaatcgtc tcaagggcat cctccgtccc gtcaagagtg
     3241 ggtgcaaatc ctccctcatc acccacggca gtgcttaagc cgcggtccct gagtactttt
     3301 ttaagcgaat ggaatatttc ggctcccatt cttagccctt ctctgaaatc cgctgcaccg
     3361 accggtctga tcatgaattc ctggaaag
//
LOCUS       DAOSPX010000339         3340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_25704_length_3340_cov_4.147032, whole genome shotgun sequence.
ACCESSION   DAOSPX010000339 DAOSPX010000000
VERSION     DAOSPX010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25704_length_3340_cov_4.147032"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PLZ75_12755"
     CDS             complement(<1..1315)
                     /locus_tag="PLZ75_12755"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOS73306.1"
                     /translation="MKNKQLILVLLAVLFHQISVGQTDTYTIKRTVFSSDFYDEFAPV
                     FYQNGLVFVTNRSSGVTDYSTSQDANFTKIFFIDTDGKKTRPRLFSKYLRTRLNDGPA
                     TFNASGDTVYFSNNLITEGNLRKISSMRNKLGIFYAVREGKKWTKIREMRFNNEWYNI
                     STPCLSPDGNRLYFASDKPDGFGGTDIYYSERKGEYWSDPVNLGPVINTAGNESYPFV
                     TAEGELYFSSDGHPGLGGKDIFFSAMKEGRWQTPVRLDPPVNSQHDDFALITDPMGRG
                     GYFTTNRDNSLDIYEFSTVSSQIFYAGLQKDNQYCFTFSDPGTIEIDTLFLKYVWDFG
                     DGIKAEGAEAGHCFPGPGKYNVRLDIADKKNGNLFFTKLMLEIDLRDVEQPYITSPDA
                     LTSGETVEFDALKSYLPGFDIADWTWDFGDGTRGEGESIKHSYDKP"
     gene            complement(1328..2245)
                     /locus_tag="PLZ75_12760"
     CDS             complement(1328..2245)
                     /locus_tag="PLZ75_12760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOS73307.1"
                     /translation="MKNLIHILIFLLLPGSLMAQLTPVTNQYILNPMTINPAYAGNRG
                     ALNVASFYRRQWVGITGAPQTMTLAVDAPFLDSKLGIGMTITNDKIGVTKETQFMSSY
                     AYKISMGQGILSLGLGAGLMTTNTAWSDLVVLDPGDDHYLVDSRVFVVPGFTFGTYYS
                     NQNFFAGISIPRLLGYNFNFNRNKYSLIIDPGNYDYLLNMGLLLNLAPKVKLLPSVLV
                     SWTPGTQFLYDINAHLNISDRMWLGATYRNDRSVAGLFQFSVNEQLKVAYTYDYDIGK
                     LGRYSNGSHEVMLRYQFTFRADAVNPMIF"
     gene            complement(2527..>3340)
                     /locus_tag="PLZ75_12765"
     CDS             complement(2527..>3340)
                     /locus_tag="PLZ75_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOS73308.1"
                     /translation="TNAVGCTSASSASVLINARPGPPTVIITDPDTICSIHTADITRP
                     EITEGSDPGLIYTYWMDPDTVRRVQDPTAVPPGIYYIKGRTTANFYTVAPVNVRADEM
                     PVADAGPDQVLGYTFKTVMNALQPESGSGMWSVDSGTGVFTAVSDANTEVRGLSLGRN
                     EFVWTVTNGVCPPASDRMVIQVNDLTIPSLITPNMDGINDFLIIQGLENLGKTELIIF
                     DRRGVEVYKDQDYNNDWYGIDYDSRPLPEDTYFYQIRTASGRNMKGFVVIRR"
BASE COUNT          861 a          794 c          726 g          959 t
ORIGIN      
        1 cgggtttatc gtacgaatgc tttatacttt ctccttcccc ccttgttccg tctccgaaat
       61 cccaggtcca gtccgcaatg tcaaagccgg gcaaatagga tttcagagcg tcaaattcaa
      121 ccgtctcccc gcttgtgaga gcatcgggag aagtgatgta tggttgttca acatccctca
      181 gatcgatttc aagcatcagt tttgtaaaaa agagattccc gttttttttg tcggcaatat
      241 ccagtctgac attatatttg cccgggccgg ggaaacaatg tccggcttcg gcaccttcag
      301 cttttattcc atcgccgaaa tcccaaacat acttgagaaa caaggtatca atttctattg
      361 tccccggatc gctgaaggta aaacaatact gattgtcttt ctgcagtccg gcatagaata
      421 tctgggatga aaccgtactg aactcatata tatccaggga attatccctg ttcgtggtaa
      481 aatatccgcc cctgcccatc ggatcggtta tcagggcaaa atcatcgtgc tgtgagttta
      541 ccggagggtc aagcctgaca ggtgtctgcc acctgccttc cttcatggct gaaaagaaaa
      601 tgtcctttcc acccaggccc ggatggccgt cggaggaaaa atacaattct ccctcggcgg
      661 tgacgaaggg ataggattca tttcctgcag tgtttatcac aggtccgaga tttacgggat
      721 cactccagta ttctcccttt ctctcgctgt aataaatgtc cgtaccgccg aatccgtccg
      781 gcttgtcaga tgcaaaatac aatctgttgc cgtcaggcga aagacacgga gtggaaatat
      841 tataccattc attattgaat cgcatctccc gtatttttgt ccacttttta ccctctctca
      901 cggcatagaa gatacccagc ttgttccgca tcgatgagat tttcctgaga ttcccctctg
      961 ttatcagatt gtttgagaaa tagacggtat caccactggc attgaatgta gccggtccgt
     1021 cgttcagtcg ggtcctcagg tatttcgaaa acagcctggg cctggttttc tttccgtcgg
     1081 tatcaataaa aaatatcttg gtgaaatttg catcctggga tgttgagtaa tcggtcactc
     1141 cggagctccg gttggtgaca aaaaccagtc cgttctgata gaaaaccggt gcaaattcat
     1201 catagaaatc cgaactgaat acagtccgtt ttatggtgta tgtatcggtc tggcccaccg
     1261 atatctgatg aaacaggaca gccagaagga caaggattag ttgtttgttt ttcatgattt
     1321 caggcactta aaaaatcatc ggattaaccg catccgctct gaacgtgaac tgatatctga
     1381 gcattacttc atgcgatccg ttggaatatc tgcccagttt gcctatgtcg taatcgtaag
     1441 tgtaggctac tttgagctgc tcattcaccg agaactggaa caatcctgca acagaccggt
     1501 cgttacggta ggttgccccc agccacatgc ggtcagatat gtttaaatgg gcatttatat
     1561 catagaggaa ctgggttccc ggagtccagc ttacaaggac cgatggcagg agcttcactt
     1621 ttggtgccag attgaggaga aggcccatat taagcagata atcataatta cccggatcaa
     1681 tgataagcga atatttgttc ctgttgaaat taaaattata acccagaagc cttgggattg
     1741 agatccccgc gaaaaaattc tgatttgagt aataggttcc gaatgtaaag ccgggtacga
     1801 caaaaactct cgaatcaaca agataatgat catcgcccgg atctagcacc acaagatccg
     1861 accatgccgt atttgttgtc attaatcccg cacccaggcc aagtgacagt atcccctgtc
     1921 ccatgcttat cttgtatgca tatgacgaca tgaactgagt ttcttttgtc acacctatct
     1981 tatcattcgt tattgtcatt cctattccca gttttgaatc aaggaaggga gcatccacag
     2041 ccagtgtcat tgtttgcgga gcacccgtga ttccgaccca ttgcctgcgg tagaatgatg
     2101 caacgttcag cgctcccctg ttgcccgcat aagccggatt gattgtcatc gggttcagaa
     2161 tgtactgatt ggtgacgggt gtcagttgtg ccataaggct tcccggcaaa agaagaaaaa
     2221 ttaaaatatg aatcagattt ttcattggaa gaaaattaat ttgttcaggg ttcaaaatct
     2281 aaggttcagg gtttctgttc attgaacctt tgaattcact tcgctttaaa aatgaaatta
     2341 ttaagtatgt atttttattt aaaatcattg tccgttcagt aatgatatcc gcaatgtcta
     2401 tttgtctttt tgtctttctc gccctggctc aagatattaa ataactttta atgaccgtcc
     2461 gcgggacgat accggtacat ctcactgctc tgcctttcac cgtgaaacaa aaaggtcttc
     2521 acgatcctat ctccgtataa caacaaatcc cttcatattc cttccgcttg ctgtacggat
     2581 ctgataaaaa taagtatcct caggaagagg cctgctgtca tagtcaattc cgtaccagtc
     2641 gttattgtag tcctggtcct tgtatacctc tacccctctc ctgtcgaata ttatgagttc
     2701 cgttttcccg aggttttcca gtccctgaat gatcagaaaa tcatttattc cgtccatgtt
     2761 tggagtaatg agagagggaa tcgtaaggtc attaacctga atgaccatcc tgtccgatgc
     2821 cggtggacac actccgttgg ttactgtcca gacaaattca tttcttccga gtgacaggcc
     2881 ccttacctct gtattggcat cgctgacagc cgtaaagaca cctgttccgg aatcaaccga
     2941 ccacatgccg cttcctgact caggctgaag ggcattcatt acagttttaa aagtataacc
     3001 cagaacctgg tcgggacctg catctgccac cggcatttca tctgccctga cgttaaccgg
     3061 ggcaacggta tagaaattcg cggttgttct gcccttgata taatagatcc cgggaggaac
     3121 agctgtggga tcttgtactc ttcgcaccgt gtccggatcc atccagtacg tgtagatcag
     3181 tccgggatca gaaccttcgg ttatttcagg ccgtgtgata tcagcagtat gaattgaaca
     3241 aattgtatca ggatcggtaa ttattaccgt tggaggtccc ggccgggcat ttataagcac
     3301 actggctgaa gaggctgagg tacagccaac tgcattggtt
//
LOCUS       DAOSPX010000340         3324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_25849_length_3324_cov_4.358214, whole genome shotgun sequence.
ACCESSION   DAOSPX010000340 DAOSPX010000000
VERSION     DAOSPX010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25849_length_3324_cov_4.358214"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLZ75_12770"
     CDS             complement(<1..687)
                     /locus_tag="PLZ75_12770"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73309.1"
                     /translation="MDRIIRFLTKRVIPFIKEKNLLGTILRIGDILAVTLAFQLSYSI
                     NYSETGGLFFSNLTILWIYLLVAPFWLMILYLLDAIDIPRTKRYSTVFFEYLQSTALV
                     FLLLLVFYFLFKLHDISRLFLIEAAGFGFLFLFLIRLAEYYLFRIYRSKGYNYRNTVL
                     IADDSSIPFIESLLENKEWGYRIILIYSSSPAVMKKYQEFIEVLPERSGKSLYDFMEI
                     EPIDEVMYVRE"
     gene            complement(854..1432)
                     /locus_tag="PLZ75_12775"
     CDS             complement(854..1432)
                     /locus_tag="PLZ75_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73310.1"
                     /translation="MRADLTYNKNIASRGVNYGFPQFLVKNNTGIELLTEKRNEDLTN
                     YLEWLNLADDPNLVVLSSLHHYFYDAEEMKNVKTVVNLIPLNELRDTKSFLHSVYTIL
                     TPKSNFIGSFLDIKNHNSFSLRKEDVDDEAVEHGILSKIPFINTLYNLMDSKTNRNLS
                     KRDVMFLLENNGFKVQDITELNKITYFHSQRV"
     gene            complement(1897..2736)
                     /locus_tag="PLZ75_12780"
     CDS             complement(1897..2736)
                     /locus_tag="PLZ75_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73311.1"
                     /translation="MTEQLNYIEERTRIYDYYRAIREDMFQQIKRNTLDSLRASYSRI
                     AELSLGKSRLNIRIDSLNMLVAGARAGLDEAIKTKNSIKVLGINVNKAAYNSVMWIIV
                     ASLAGTLLIGFLIFRRNLIVAKRTRTDLEELKAEFEEYRKQSRLAREKMSMDHFNEIR
                     RLRARGTEHKGQGNKPDRTDDIMHFRHPEAAPASKPAGQNKSRNTVNEAAQNESTAES
                     EDFPGEAVPVTSADTELTTKEDSTPETKAGSPDIPDKTKTSRKKKSPGKANQNGDQAN
                     LAL"
BASE COUNT          976 a          621 c          643 g         1084 t
ORIGIN      
        1 ttctcttaca tacattacct cgtctatagg ttctatctcc atgaaatcgt agagggactt
       61 tcccgacctc tccggcagga cctcaatgaa ctcctggtac tttttcatca ctgccgggga
      121 actggaataa ataagtatta tccggtatcc ccattccttg ttctcaagaa ggctctcaat
      181 aaatggtatt gatgaatcat cagctatcag taccgtattg cggtaattat aacctttcga
      241 gcggtatatc cggaagagat aatattcagc gagtctgatc aggaaaagga aaaggaagcc
      301 gaaaccggca gcttctatga gaaacaagcg tgaaatatca tgcagcttga acagaaaata
      361 gaaaaccaaa agcagcagga agacgagtgc agtggactgc aggtattcga aaaataccgt
      421 gctgtagcgt tttgttctcg gtatgtcaat agcatcgagc agataaagga tcatgagcca
      481 gaaaggagcg accagaagat atatccacag tattgttaga tttgaaaaaa acagtccgcc
      541 cgtttcagaa taattgatag aatatgaaag ctgaaatgcc agagtaactg ccaggatatc
      601 accaatccga agtatggtgc caagaagatt tttctccttg atgaagggga ttactctttt
      661 tgttaaaaat ctgattatac gatccattat actgacacta ctccaacagg tttatacgtg
      721 ataaaattta aaaggtttat tgtgtaagcc tgcatttatc tgattcctta gagaagcttt
      781 gaattatcta ctgataaata taaaaaaaaa gtcgttgtga acgactttca gatgaatatc
      841 aaatgagcat ttcctatact ctttgagaat gaaaatatgt gattttattc agttcggtaa
      901 tatcctggac cttaaaacca ttattctcaa gcagaaacat aacatctctc tttgagagat
      961 tccggttggt ttttgaatcc ataagattgt aaagcgtatt tatgaaagga atttttgata
     1021 gaattccatg ttcaacggct tcatcatcaa catcctcttt tcttaaggaa aagctgttat
     1081 ggttttttat atccagaaaa ctacctatga aattactttt aggtgtaaga atggtgtaga
     1141 ctgagtgcag gaaactcttt gtatcacgta attcattgag tggaatgagg tttacgacag
     1201 ttttaacgtt cttcatctct tcggcatcgt aaaaatagtg atgcaatgac gaaagaacaa
     1261 ccagattggg atcatcagcc agattcagcc attcaagata atttgtcaga tcctcgtttc
     1321 ttttttcggt aagtaattca attcctgtat tgttttttac taaaaactgg gggaaaccat
     1381 aatttactcc tcttgatgca atattcttat tataagtaag atcggctctc attacttttc
     1441 gcgtttttca ggttttatga ggtaaacaaa tattaatgaa attagtttaa atatcaacac
     1501 gggtcaagta atatgactca tgtgagctgc aaacataata caggaagcgt gccagataat
     1561 caatgttatc agcacgctgg tgtaaattat taacaggttt gtcagaaaat ttaatatgtt
     1621 cgtcaaaaag cactgaaatt gctgaatctg acggcagttt taagactgat ttttaaagaa
     1681 ataggactaa atatctgtat tacagaaaga tgaaatcttg attttaacta aaaatatcaa
     1741 taaagaaaaa ttcaggattt tgacgaacgg ctcttttatg gtgacgaaag ttttgagaat
     1801 ctggataaca ggaaggcatg tacatcagat tcttacatgc cggatcccct gttcgcagct
     1861 tatttatgaa ttacagcctg gtttcccggc tgaggtttac aaggcaagat ttgcctgatc
     1921 cccgttttgg ttcgcttttc ccggggattt tttctttcgg cttgtttttg ttttatcagg
     1981 aatatccgga ctgcctgcct tcgtttccgg cgtactgtct tccttagttg tcaattccgt
     2041 atcagcgctt gttacaggta ctgcctctcc cggaaaatct tctgattctg ccgtggactc
     2101 gttttgggct gcctcgttca ctgtattgcg gcttttattt tgcccggccg gtttagaagc
     2161 tggtgcggct tccggatgtc tgaaatgcat aatatcatcc gtcctgtccg gcttgttgcc
     2221 ctgtcccttg tgttctgtgc cccttgccct gagtctgcgg atctcgttga aatgatccat
     2281 tgacatcttc tcccgcgcca ggcgggactg cttcctgtat tcttcaaatt cggccttcag
     2341 ttcttccaga tcagttcttg tacgtttagc cacaatgaga tttcgtctga atatcaggaa
     2401 tccgattaag agagttccgg caagagatgc aacaataatc cacattaccg aattataggc
     2461 agccttgttg acatttatgc caagaacttt aatgctgttc ttggtcttta tggcctcatc
     2521 cagaccggct ctggcaccgg ctacaagcat attcagggaa tctattctga tgttgagtct
     2581 gcttttcccc agactgagtt ctgcaatcct tgaatatgag gctctcagac tgtcaagcgt
     2641 attccttttt atctgctgga acatgtcttc ccgtattgca cggtaataat cataaatcct
     2701 ggttctttcc tctatataat tcaattgttc cgtcatcttt ccctgtttca gaacctccgg
     2761 catttctgcc tgacctgtgc agacgggtct gaatacggat agcagaataa ttaataaaat
     2821 agctcttaaa atattataat tcattctctt tgacagttag cggtaatatg atctcaaaaa
     2881 ttgcctttat tgagctaacg aatttaaaga ataattattt taaagaaaaa tgaagtattt
     2941 aatttgtttt aatccggagt atatagaata tcattgtgtt acattgatga tgcgttttct
     3001 cattcacgta ataaattcat gattattcat gtgagataat cattaaccag tgaatgaaca
     3061 atgaaatcgt gccttgataa tgaattgaat atgtgtaaac taaatgtttg tcaggaaacc
     3121 ggattaagcc ggatttataa gaaacagctg aaaattaatt cgggaacaat ttccggggag
     3181 tcaggcttta acaatgtaat aatctttcag aatattgctg taaaatattt cttattgtta
     3241 aattcgtagt gttaaaagag ttatctatga aaaaaccatc tcttttcata tttcttgtgc
     3301 tgatagttcc ggcttctgta attt
//
LOCUS       DAOSPX010000341         3313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_25971_length_3313_cov_4.864641, whole genome shotgun sequence.
ACCESSION   DAOSPX010000341 DAOSPX010000000
VERSION     DAOSPX010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25971_length_3313_cov_4.864641"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLZ75_12785"
     CDS             complement(<1..225)
                     /locus_tag="PLZ75_12785"
                     /inference="COORDINATES: protein motif:HMM:NF027542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M64 N-terminal domain-containing
                     protein"
                     /protein_id="HOS73312.1"
                     /translation="MRIFSALSLVFISLTMTAQHSFNSYFTDKVLRFDYMLAGNHEKT
                     LVYPVGMKEEPFYGGSKTQLTDPFGYGNFRY"
     gene            422..1639
                     /locus_tag="PLZ75_12790"
     CDS             422..1639
                     /locus_tag="PLZ75_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOS73313.1"
                     /translation="MADLNTRISDFLWKNEIERRVVAQKDPFWTALCATGTELWLDTG
                     DMEEAGANWTAEMSALTTNNTLLNNEIQKGIYDVLISEAKHLVSHLPVNERVKEIAFI
                     LNARHGLRLASRFGGLVSVELHTDAANDIDAIEYYGRRFHELCPDQFLIKVPFTAEGL
                     IGARKLKEAGIRVNFTLEFSARQNVLVTGVARPDYVNVFLGRIGAFMADNKLGDGSGA
                     GEMAVISSQNWVTGLSAKNPWQTKLIAASLRNYRQLELLAGADVFTIPPRVAAEGRKN
                     LPGTFFPRMHENYEVSMFASAAEAHIEKFWTVDDNVLNLVDRLADNFPETGAELIQIA
                     REEGCADMFPSLTREEKSAVAADGKIPVYSRWKDRIREGSIAADTLLSLAGLASFTAD
                     QKMLDDRILEIIS"
     gene            complement(1641..2381)
                     /gene="rlmB"
                     /locus_tag="PLZ75_12795"
     CDS             complement(1641..2381)
                     /gene="rlmB"
                     /locus_tag="PLZ75_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOS73314.1"
                     /translation="MKAESEYIFGLRPVIEAIRAGKQIDRLLVKEGLRGELYHELMTE
                     VRKHKIVWQTVPAGRIELVTRKNHQGVLAWLSLVEYQDLSVLLPMIFEKGEDPLIVAL
                     DGVSDVRNFGAVVRSADCLGAHAVLIPEKGSARITADAVKASAGALNTLPVCREKSLV
                     KAIRFLKESGLKVICAAEKSEKKPADCDLTGPAVLVMGSENRGISREINALADNITGI
                     QMSGNIASLNVSVAAGILLYEINRQRRG"
     gene            complement(2466..>3313)
                     /locus_tag="PLZ75_12800"
     CDS             complement(2466..>3313)
                     /locus_tag="PLZ75_12800"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOS73315.1"
                     /translation="KYNFTARGHFARNELFNPIVKKNEFVNLIYLKPPADSLFISYYR
                     KLNEIPDPPSMLLPEKTIDYPPDTVIALSYSDTLPLMFPNEGVYLCSIDRKINDGFTF
                     LNLGPRFPQMDEPLTMIEPLAYIGTPDEIFRMKTAERPKMALDEFWISCGGNVDKARE
                     LIRIFYTRVLFANYYFTSYKEGWRTERGMIYIIYGPPDKVYKTNEGENWGYRKPVIKS
                     SWGTRYRVKEDYLFFNFKKKENVFSDNDFFISRSETLITMWDQAVASWRKGIVFRFDN
                     PEDLF"
BASE COUNT          829 a          721 c          859 g          904 t
ORIGIN      
        1 atatctgaaa ttgccgtatc cgaagggatc ggttagctgt gtttttgatc ctccgtagaa
       61 aggctcctcc ttcatgccaa caggataaac aagcgttttt tcatgattac cggcaagcat
      121 gtaatcaaag cggagaactt tatcggtgaa ataactgtta aagctatgct gggcagtcat
      181 tgtaagggaa atgaaaacca gggacaaagc ggaaaagatt ctcatggcgg gttttatttg
      241 ataaacgatg gataaagcta tgatattgga aggaggaatc aaaaccggac cggacaggga
      301 gttaataatt tctctaaatt tgcggaatat tttaattgcg gagaggtgaa agttccggat
      361 tggtaccggt gaaaatcccg gatcccttac cgggagcagg aaaaattaaa actttgaaat
      421 tatggcagat ctgaataccc gaataagcga ttttctctgg aagaatgaga ttgaaaggcg
      481 tgttgtggca caaaaggacc cgttctggac tgccctttgt gcaacaggca ctgagctgtg
      541 gctcgatacc ggtgacatgg aggaagccgg tgcaaactgg acagccgaga tgagtgccct
      601 tactacaaac aatactctgc tgaacaatga gatacagaaa gggatatatg atgtgcttat
      661 tagcgaggca aagcatctgg taagtcatct gcctgtcaat gagagagtaa aagagatagc
      721 attcattctc aatgcaagac atggcttgcg tcttgcttca agattcggcg ggctggtaag
      781 tgtggaactg cacactgatg cggcaaatga tattgatgcg attgaatatt acggacggag
      841 gtttcatgaa ttatgtcctg atcagtttct gataaaggta ccgttcacgg cagaggggct
      901 tatcggggcg aggaaactga aggaagcggg tattcgcgtc aattttaccc ttgaattcag
      961 tgccaggcag aatgttcttg tgacaggagt ggcacgtccg gattatgtca atgtatttct
     1021 gggaaggata ggtgccttta tggctgacaa taagctcggg gacggcagcg gggcgggaga
     1081 aatggcggta atatcgtcac agaactgggt aaccggcctg tcggcaaaaa atccctggca
     1141 gacaaagctg atagctgcca gtctgaggaa ttaccgtcag cttgaactgc ttgccggagc
     1201 agacgttttt accattcctc ccagggttgc cgcggaaggc aggaagaatc tgccgggaac
     1261 atttttcccg aggatgcatg agaactatga ggtcagcatg tttgcctccg cagcagaggc
     1321 acatattgaa aagttctgga cagttgacga caatgtcctg aaccttgttg acaggctggc
     1381 tgacaatttt ccggagacgg gagctgaact cattcagatt gcacgggagg agggctgtgc
     1441 cgacatgttt ccttcactca caagggaaga aaagtctgca gttgccgcgg acggaaagat
     1501 tccggtttat tccaggtgga aggacaggat cagggaggga agcattgcgg ctgacacttt
     1561 gctgagtctt gccggactgg cttcctttac tgcggatcag aaaatgctgg atgacagaat
     1621 actggagata atatcctgaa tcagcccctg cgctgccggt ttatttcata gagcaagatt
     1681 ccggctgcga cagatacatt cagtgatgca atatttccgc tcatttgtat tcccgtaata
     1741 ttgtcggcca gtgcattgat ctcccggctg atccccctgt tttccgagcc catgaccaga
     1801 actgcaggtc ctgtaagatc gcagtctgcc ggtttctttt cagatttttc ggctgcgcat
     1861 attactttca gaccggattc cttaaggaag cggatggctt ttaccagtga tttttccctg
     1921 cacacgggga gggtattcag agctccggca gaagctttaa cggcatctgc ggtgattctt
     1981 gccgatccct tctccggaat aagcactgca tgggctccca ggcagtcggc ggaacggacc
     2041 actgctccga agttccttac atccgacact ccgtcgaggg ccacaatgag agggtcctca
     2101 cccttttcaa atatcattgg aaggagaacg gataaatcct ggtattcaac aagtgacagc
     2161 catgccagaa cgccctggtg atttttcctt gtcacaagtt caattcttcc ggcggggact
     2221 gtctgccata cgattttatg tttccttact tcggtcatta gctcatggta caattctcca
     2281 cgcaggccct cttttacaag gaggcggtct atctgtttac cggcccttat ggcttcaatg
     2341 accggcctca ggccaaagat atattctgat tccgctttca tttttagcgg gggtttttca
     2401 ttactcagga tgttttccat ttccttgcgg tggatttccg gcttttccgg ggctctgcca
     2461 ttgttttaaa aaagatcttc gggattatca aatcgaaaaa cgataccttt cctccagctt
     2521 gcaacggcct gatcccacat ggtgataagt gtttcgctcc tgcttatgaa aaagtcgtta
     2581 tcggagaaaa cgttctcttt ctttttgaaa ttaaagaaga ggtaatcttc ctttaccctg
     2641 tacctggttc cccaggagga ttttatcacc ggtttccggt atccccagtt ttctccctca
     2701 tttgttttat agaccttgtc gggagggccg tagattatgt atatcattcc cctttcggta
     2761 cgccatcctt ccttgtatga tgtgaagtaa tagtttgcaa acaataccct ggtgtagaat
     2821 atcctgataa gttccctggc cttgtccaca tttcccccgc aggagatcca gaattcatca
     2881 agggccattt tcggccgttc tgccgtcttc attctgaata tttcatccgg ggtgccgata
     2941 taggcaaggg gttcgatcat agtcagcggc tcgtccattt gcgggaagcg cggacccagg
     3001 ttgaggaaag taaaaccgtc gtttatcttt ctgtcgatac tgcacaggta tactccctcg
     3061 ttcgggaaca tcaggggaag ggtatcggaa taggacaggg ctattacggt atcgggggga
     3121 taatcaatgg ttttttccgg cagcagcatt gagggcggat cgggtatttc attcagtttc
     3181 ctgtaatacg agatgaacag ggaatcggcc ggtggtttaa gatagatgag attgacaaat
     3241 tcgtttttct tcactatggg attgaagagt tcatttctgg cgaagtgtcc ccgggcggtg
     3301 aagttatatt tat
//
LOCUS       DAOSPX010000342         3306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_26044_length_3306_cov_4.092587, whole genome shotgun sequence.
ACCESSION   DAOSPX010000342 DAOSPX010000000
VERSION     DAOSPX010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26044_length_3306_cov_4.092587"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLZ75_12805"
     CDS             <1..745
                     /locus_tag="PLZ75_12805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS73316.1"
                     /translation="ERLSEIEYYSSAGRVEEASASLNELMKVYPDSENTKYAEALVHM
                     SAGRLTSAVKILTGLAAADQANEKYLRTLGRVQDALGNHAGASVTWSKLIGLEVADPD
                     LYLKRAESYSKTGENDKAVADLEKYLTLFPGEKNALRLAGRISAASGDNIRALSYFSE
                     NLRNNPNDPQCYIDRANSYLSARSWDWAVLDYAMALDLDPGNPDTWLNKGIALLNAGK
                     TDDACHDFRKSFRMGNKRAAEYISRHCIK"
     gene            complement(751..>3306)
                     /locus_tag="PLZ75_12810"
     CDS             complement(751..>3306)
                     /locus_tag="PLZ75_12810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS73317.1"
                     /translation="RLLELLNLIHQSGEAQKLSVSPMNDDSEGFYLGFSISPGYIIVT
                     WEPGENQKKNAEIRRSRFEFENFADMLPEMIYELDLTGKLVFANTQALRFGGYTAEEM
                     RKGLHISQFFPEHYKKMLENLAAIRNHEDISSNEYYIRKKDGTTVPIVTHSFAIFHED
                     KIIGYRGTVTDISKRKKYEEQITREKAFLEHLIDSTPLAIVISDVPGKITMVNKEFTN
                     LFGYTSAEAINRNINDLVVPDEFREEAESIDKLAAARHRVIRETIRKDKSGNRIQVSL
                     TSTAIVVNYEPAAFVGIYRDITTEKKNALQQEVLYNISSAALQQTDLEELYPTIEREL
                     GKIWSTNNFYIALYDSKTETLTMPFFADEKDTFREVSAHKTITGWVIRNKKSVLLKEK
                     DLAELEEAGEIELIGTQCKVWMGVPLQVDNDVIGVICLQDYYDEDRFSPDDLYVLDFI
                     ANQTAIALQRRIMLDNLITARQKAEEAAQSKQIFMSTMSHEIRTPLNEVIGITNLLLQ
                     GNPRDDQMDYIKTLRFSGNHLLTLVNDVLDYNKMESGKIVFEQIRFNLNDFLDEIMRS
                     YSFRSKAKNLDFDIVKVGSLPVNVIGDQIRLNQILSNLLSNALKFTKEGGISVIIREV
                     ERNNSQSKLEFSVRDSGIGIPFEKQAAIFENYAQASSDTTRRYGGTGLGLAICKKLVE
                     LQGGTIGVQSDPGKGSTFSFTLSFEDVTDGDARDEEIQVSETYTGLEGKKILVAEDNK
                     INFFVVNKFLSGWGVIVAHAEDGQLAIEKLENEDFDLILMDLHMPIMDGIEATQVIRS
                     SEDPRIRDIPIVALTAAIMSENHDRLDQLNINDYVLKPFKPHDLFERIRKHIR"
BASE COUNT          777 a          783 c          775 g          971 t
ORIGIN      
        1 ggagaggctt tcggaaatag aatactattc ttcggcgggc agggtggaag aagcctcagc
       61 ttcactgaat gagctcatga aggtttatcc ggattctgaa aacacgaaat acgccgaagc
      121 acttgttcat atgtcagcag gccgtctcac ctcagcagtc aaaatactca caggactggc
      181 cgccgcggat caggcaaatg aaaaatattt gagaaccctt ggcagggtgc aggatgctct
      241 cgggaaccat gcgggtgcat cagttacctg gtcaaagctt atcggtttgg aggtggctga
      301 tcccgatctc tacctcaaac gggcggaatc atacagtaaa acaggcgaga acgacaaggc
      361 agtagctgat ctggaaaaat atctgaccct tttccccggg gaaaaaaatg ctctgaggct
      421 tgccggaagg ataagtgcgg cttccgggga taatatcagg gcactgagtt atttcagcga
      481 aaacctcaga aacaacccca atgacccgca gtgctatatc gacagggcca attcatatct
      541 ttcggccaga tcgtgggact gggccgtact ggactatgcc atggcactcg atcttgatcc
      601 cggcaatccc gacacctggc tgaacaaggg cattgcactt ctgaatgcgg gaaaaacgga
      661 tgatgcctgc catgatttca ggaaatcctt caggatgggc aacaaaaggg ctgcagaata
      721 tatcagcagg cactgtataa aatagggatc ttaccttata tgtttcctga ttctttcgaa
      781 aagatcgtga ggcttgaacg gtttgagaac atagtcattt atgttcagct gatcgagcct
      841 gtcatggttt tccgacatga ttgccgcagt aagggcaact atcggtatat cccttatcct
      901 cggatcctct gaacttctga ttacctgagt ggcttcaatg ccatccatta taggcatgtg
      961 aaggtccatc agtatcagat caaagtcttc attctcaagt ttttctattg caagctgccc
     1021 gtcctcggca tgagctacaa tcactcccca tccgctcagg aacttgttta caacgaagaa
     1081 gtttatttta ttgtcttcag caaccaggat tttcttgcct tcaagacccg tataagtttc
     1141 gctgacctgg atttcctcat cccgtgcatc cccgtctgtt acgtcttcaa aactgagtgt
     1201 aaaactgaat gttgaccctt ttccgggatc actttgaact cctattgttc cgccctgcag
     1261 ttcaaccagc tttttgcaga tagccagccc gagtccggtg ccaccgtatc ttctcgtggt
     1321 atccgacgaa gcctgggcat agttttcaaa tattgccgcc tgtttttcga aggggatgcc
     1381 gataccgctg tccctgactg aaaattcaag ttttgactgg ctgttgtttc tttcaacctc
     1441 cctgataatc acacttatac caccttcctt ggtgaatttc aatgcatttg acaggaggtt
     1501 tgagaggatc tggtttagcc tgatctggtc gccgatgacg ttaacaggca ggctgcccac
     1561 cttaactatg tcgaaatcaa gatttttggc ctttgatctg aatgaatagg atctcatgat
     1621 ctcatcaagg aaatcgttca ggttgaaacg tatctgttca aacactattt tgcccgattc
     1681 catcttgtta tagtccagaa catcgttgac cagggtgagc aggtgatttc cggagaatct
     1741 gagtgttttg atgtaatcca tctggtcatc gcgcggattt ccctgaagca ggaggtttgt
     1801 gataccaatg acttcattca ggggggtccg gatctcatgg ctcattgttg acatgaatat
     1861 ttgcttggac tgggcagcct cctcggcttt ctgcctggcc gtaatgaggt tgtcaagcat
     1921 tattcttctc tgaagcgcga tagccgtctg gttggcaata aaatcaagga catacagatc
     1981 atccggcgag aacctgtcct cgtcataata gtcctggagg cagatgactc caatcacgtc
     2041 attgtcgacc tggaggggaa cacccatcca aaccttgcat tgtgttccta tcagttctat
     2101 ttcacctgct tcctcaagct ctgcaaggtc tttttccttc agaagtaccg atttcttgtt
     2161 ccggatgacc cagcccgtta ttgttttgtg tgctgacact tccctgaagg tatctttctc
     2221 gtcggcaaaa aacggcatgg ttagtgtttc cgtcttgctg tcgtagaggg caatgtagaa
     2281 attgtttgta ctccatattt ttccaagctc ccgttcaatg gtggggtaca gctcttcgag
     2341 gtccgtctgc tggagggcgg cagatgagat attatacagg acttcctgct gaagggcatt
     2401 cttcttttcg gtggttatat ccctgtagat gccgacaaaa gccgcgggtt catagttcac
     2461 gacaattgcc gttgatgtga gggatacctg aatacggttg cctgatttgt ccttcctgat
     2521 tgtctccctt attacccggt gacgtgctgc ggcaagtttg tctatgcttt ccgcctcttc
     2581 cctgaattca tcgggcacaa ccaggtcgtt aatattcctg tttatggctt cggctgaagt
     2641 gtagccaaaa aggtttgtaa attccttgtt gaccatggta atctttccgg gaacatcgga
     2701 aattactatt gccagagggg tggaatctat cagatgttca aggaaggcct tctcccttgt
     2761 gatctgttct tcgtacttct tacgcttgct tatatcggtg accgttcccc tgtaaccgat
     2821 tattttatct tcatggaaga tagcaaatga atgagtcaca atgggtactg ttgtgccgtc
     2881 cttttttctt atatagtact cattgcttga gatatcctcg tgatttctga ttgctgcaag
     2941 gttttccagc atttttttat aatgctcggg gaaaaactgt gagatatgca ggccctttct
     3001 catctcttcc gcggtatatc ccccgaatct gagtgcctgt gtgttggcga agaccagttt
     3061 tccggtcaga tcgagctcat agatcatctc gggaagcatg tctgcaaaat tctcgaattc
     3121 aaacctgctt ctcctgattt cagcattttt tttctggttt tctccgggct cccaggttac
     3181 aattatatag cccggtgaaa tggagaatcc caggtagaaa ccctcggaat cgtcgttcat
     3241 gggagagact gacagtttct gtgcctcgcc cgactgatgg atcaggttca gaagttccag
     3301 gagcct
//
LOCUS       DAOSPX010000343         3298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_26132_length_3298_cov_4.239593, whole genome shotgun sequence.
ACCESSION   DAOSPX010000343 DAOSPX010000000
VERSION     DAOSPX010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26132_length_3298_cov_4.239593"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLZ75_12815"
     CDS             complement(<1..464)
                     /locus_tag="PLZ75_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73318.1"
                     /translation="MAGKIIRQLNGLIPVARLMRFLVGISVTVQLIVIIYNNLSGFYP
                     LESFTHFLLRLLRGVLLTMPAAVMIAVPDLLVIDWLNRLFPWNRKALERVLIQFPVSV
                     TLAAAVSVVITLTANTIMAYSEPLNLVLLYNALIYSVVNIILCIYIFKASIV"
     gene            complement(736..1317)
                     /locus_tag="PLZ75_12820"
     CDS             complement(736..1317)
                     /locus_tag="PLZ75_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOS73319.1"
                     /translation="MKTKVFFISALMAILPLGLVAQNSGKRSALEMNGALSLSSFFSG
                     ETVTGAGAEALFHYRLAKQLGIYGGWGYNVFRNDYPPAGYRCDFEETGYSIGLQYKHK
                     IEFFNSSLFVRGGCLYKHIEIEDPSGELLFDTTHRPGWQAGCGIDIPVAEKWSIVSEA
                     RFSSLNERKAQTNTSPIPVRNYFSIRLGILKKF"
     gene            complement(1551..2333)
                     /locus_tag="PLZ75_12825"
     CDS             complement(1551..2333)
                     /locus_tag="PLZ75_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOS73320.1"
                     /translation="MKLRKEELKEFLDEKAEEYNRPSFIEDDPISIPHRFSGKEDREI
                     SGFLTAAISWGRRSMILANAAKMMDLLNNSPYEFIMNAGETDFAGMKGFVHRTFDSAD
                     LIYFIKALKHIYSHRGGLEEIFTGYMTEDSLQPAIHELHNIFFELPHSKRTEGHVPDP
                     YKGSAAKRINMFLRWMIRKDNRGVDFGIWNRIPPSILSCPLDVHSGNVARKLGLISRK
                     QNDAKAVAELDGILRIFDPEDPVKYDFALFGLGVAEGFSRES"
     gene            complement(2353..3006)
                     /locus_tag="PLZ75_12830"
     CDS             complement(2353..3006)
                     /locus_tag="PLZ75_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS73321.1"
                     /translation="MIRTAGITKSFGDLKVLKGIDLDIKDREVVSIVGASGAGKTTLL
                     QIIGTLDYPDGGSVFYNGTDVTRLRGKNLAAFRNTNIGFVFQFHQLLPEFTALENVCM
                     PAYISGKGRAEAEAKAVELLGFLGLADRMEHKPSELSGGEQQRVAVARALINDPSVIL
                     ADEPSGNLDSENKNELHKLFFRLRDNFGQTIVIVTHDRQLAEMSDRILQIRDGLIIN"
     gene            complement(3061..>3298)
                     /locus_tag="PLZ75_12835"
     CDS             complement(3061..>3298)
                     /locus_tag="PLZ75_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73322.1"
                     /translation="TGSIDKEISSVSDSIDRDVNEISGNTGGEVSGVSESISGDAAAR
                     PDDVSGAENVNEETAGIKKTSEDSASGDYYEYTD"
BASE COUNT          864 a          788 c          719 g          927 t
ORIGIN      
        1 ctcacaatgc ttgccttaaa tatatatata caaagaatga tgttaacaac agaatagata
       61 agggcattat agagaagcac aaggttcagt ggttctgaat atgccatgat ggtattggca
      121 gtcagtgtta tcactactga gactgctgca gccagggtca ccgataccgg aaactgaatc
      181 agcacccttt ccagtgcctt ccggttccag gggaataacc tgttcaacca gtctattaca
      241 agaagatcag gtacagctat cattacagca gcaggcatgg tgagcagcac tccgcggaga
      301 agcctgagaa ggaaatgggt gaagctttca agagggtaaa acccgctgag attattataa
      361 atgataacga taagctgaac tgtcaccgat atcccgacaa ggaacctcat cagccttgcc
      421 accgggatca gcccgtttaa ctgccttata atttttccag ccatttcagt ctgttgatca
      481 gttcttacag caaattgata ttaaaaacaa aattagggaa agaactttta ctctgatcat
      541 aaacacctgc ctatctgatg agctgcaact gcattctgat atatccggta ccattcatac
      601 tgagataacg catttccctc tcgttccaat tatattacct gtcaggcgga tggcactcac
      661 gatgatcatt gcggcaacaa tcacgatcag aactgttttc ctgaaattca ttttaatttt
      721 ctgttgaaag ttgattcaga attttttaag aataccgagt ctgattgaga agtaatttct
      781 cacaggtatt ggagaagtgt ttgtttgagc ttttctttca ttcagggaac tgaacctggc
      841 ctcagagaca atagaccatt tttctgccac cggaatatca ataccgcatc ctgcctgcca
      901 tccgggtctg tgagtggtgt cgaaaagcag ttccccggaa gggtcttcga tctctatgtg
      961 tttgtagaga cagcctcccc tgacaaagag tgaactgtta aaaaactcaa tcttgtgctt
     1021 gtactgaagt ccgatgctgt agccggtctc ctcgaaatcg catctgtatc cggccggagg
     1081 ataatcattc ctgaaaacgt tgtagcccca tcctccgtag atgccgagct gctttgcaag
     1141 ccggtagtga aaaagcgctt cggcaccggc acctgttaca gtctcaccgg agaagaagct
     1201 tgaaagggaa agcgctccat tcatttctaa tgcactcctt tttccggagt tctgcgcaac
     1261 aagacccaga ggcaggatgg ccatcagggc tgaaataaaa aatacttttg tcttcatttt
     1321 gatttgtttt aaaagattta gaattatttg tcgaagcaaa agtagatgca gcatggcggt
     1381 acggaaaata aaagatgcca ggagcgggaa aacaattgct gaaggtgtca gcaaagggta
     1441 tgagagtgtc aggattaacg gaataaagat tccaccttca atatttcagt ttagctttca
     1501 tttgtcttca cagcatcagc cgttggtcca tggcagccgg taagggaatt tcatgattcc
     1561 cgggaaaatc cttccgcgac acccagtccg aaaagtgcaa aatcatactt tacgggatct
     1621 tccgggtcaa atattctcag aataccatcc agctcggcaa cagcttttgc atcattctgt
     1681 ttccggctga tcagacccag cttgcgcgca acgttacctg aatgaacatc cagcgggcat
     1741 gaaagtatgg aaggcggtat cctgttccag ataccaaaat ccactcccct gttgtctttc
     1801 cttatcatcc atctgagaaa catgtttatc ctttttgcag cagagccttt gtaaggatcc
     1861 ggaacatgcc cctctgtcct tttgctgtga ggcaattcaa aaaagatatt atgcagctcg
     1921 tgtatggctg gctgaaggga atcttccgtc atgtatcccg tgaatatttc ctccagaccg
     1981 cccctgtggc tgtaaatatg tttcagggcc tttataaaat agatcaggtc agctgaatcg
     2041 aaggtacggt gaacgaagcc cttcatccct gcaaaatcag tttcaccggc attcattatg
     2101 aattcatagg gggaattgtt gagcaaatcc atcattttcg ccgcatttgc cagaatcata
     2161 ctgcgcctgc cccaggaaat ggctgctgtg aggaagcccg aaatttccct gtcctccttc
     2221 ccggaaaacc tgtgaggtat gctgatggga tcatcctcta tgaaggaagg cctgttatat
     2281 tcttccgctt tttcatcaag gaactctttc agttcttctt ttctcagttt catatttctg
     2341 atcctgacag gcttaattta tgatcagtcc gtccctgatc tgaaggatcc tgtccgacat
     2401 ttctgccagc tgcctgtcat gagtcacaat aacaatggtc tggccgaaat tatccctcag
     2461 tctgaaaaag agtttatgca attcattttt attttccgaa tccagattgc cagagggttc
     2521 atcagccagt atcacggagg gatcgtttat cagggccctt gcaacggcaa ctctctgctg
     2581 ttctcctccg ctgagctctg aaggtttgtg ctccatccgg tcagccagtc ccaggaatcc
     2641 aagcagttca actgcctttg cctcagcctc agccctgcct ttaccggaaa tataggccgg
     2701 catgcagacg ttttcaagtg cagtgaactc aggcagcaac tggtgaaact gaaaaacaaa
     2761 accgatgttg gtattacgga aggctgcaag attttttcct cttaatctgg tgacgtcggt
     2821 cccgttgtaa aagaccgatc cgccgtcggg atagtcaagg gtcccgataa tctgaagcag
     2881 tgtggttttg ccggctccgc tggctccgac aatggaaacg acttcacggt cctttatgtc
     2941 aaggtctatt cctttcagga ccttcaggtc tccgaatgat tttgtgattc cggctgttct
     3001 tatcatgaag ggagggtatt gctggcactg aatatcatca acatgaacag aattcagtat
     3061 tcagtcggta tattcataat aatctccgga tgcagagtct tctgatgttt tcttaatccc
     3121 tgctgtctcc tcattcacat tctctgcccc ggatacatca tcaggccttg ctgcagcatc
     3181 acctgaaatg ctttcactga ctcctgagac ttccccgccg gtattgccgg agatctcatt
     3241 aacatccctg tcaatgctgt cggatacgct gctgatttcc ttgtcaatac tgccggta
//
LOCUS       DAOSPX010000344         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_26156_length_3296_cov_4.129590, whole genome shotgun sequence.
ACCESSION   DAOSPX010000344 DAOSPX010000000
VERSION     DAOSPX010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26156_length_3296_cov_4.129590"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PLZ75_12840"
     CDS             <1..1195
                     /locus_tag="PLZ75_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73323.1"
                     /translation="SLPGVPNASPAVDVTIPFSEIKLYSADYYTGTAGGSVAGSRSKW
                     ARLAAAAQATSTAAGKAASISTPGSRLGGTFSQQETVNRLNEFRNSLTVYRGPIEFIT
                     FTEPSSGSTAVSEESTDTRAAAARAAVARGAVAGKAAAQVPARTAAVFPAEKLLPQRG
                     NVDSPLGLFSVGGLTFEPDQKVKARIEVKGFTVESEWIETEGILVYPIEHEGFRSSYK
                     PGSETFSADNSFVVVSAIHGETAPSGNVKILSGSDAPHSSLTAPQAVPEFPQAVRAKV
                     WFSKDVPLQPLEGHPGSAIASTGPWSVTYAYSSPLDAINPRKNGKHPFEMVTYVFKGK
                     DHGYSDYAGECISCTSPVNIIEKMGRDAMPGTSGRTGRPAMQQNANVPREAPQKQIKK
                     PVMVR"
     gene            1313..2266
                     /locus_tag="PLZ75_12845"
     CDS             1313..2266
                     /locus_tag="PLZ75_12845"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOS73324.1"
                     /translation="METRIIISQIFMLAVVVLIGAISARFRVITTEIKDMLSKLIFNI
                     SLPLMLFTNFLRIEATPKLIANSLTVLGISGFVILLLFIIGWITTRLMRFTTSESAVF
                     RAHSMFGNTIFLGFPLIHALYGAEGLLYATMFQLVSNIIMWTAGVVVLTHGNGVSWKK
                     SILKVMNPNTIATLAGLVFFMFSLKLPEIIVRPLAELGSANTWLSMLYIGAMLVFANV
                     GDLLKTKSLYIISFNRLILAPAILVAVFYVFGVFAGLAPDKLVTSVIILEASMPCMAT
                     VVIMAKEFGADDHLAVGNVFVSTILSIVTLPLVLMAINSFL"
     gene            2520..2789
                     /locus_tag="PLZ75_12850"
     CDS             2520..2789
                     /locus_tag="PLZ75_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73325.1"
                     /translation="MPRFDFEKLNANLEIQGFDDSLTREKIMNAIRAVPEKTYDKAVV
                     FLGIATLIVALGGIILAAMGKTVPDALWAALGAGIGGLAGIFTGK"
     gene            complement(2885..>3296)
                     /locus_tag="PLZ75_12855"
     CDS             complement(2885..>3296)
                     /locus_tag="PLZ75_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73326.1"
                     /translation="SKGEEKEEFMLPFTNTGTRARNDGLNIPGSLVRMILRKDHPLTW
                     GMPGETNIFYRGNPLFNTSMPRFDMDRRVIGAFPEKDILVSGYSEKEEIISEKPAMIW
                     IRKGKGELILFAFSPQFRASTQGTFKLLFNSMLQ"
BASE COUNT          820 a          801 c          850 g          825 t
ORIGIN      
        1 ttcattgccg ggtgtaccca atgccagtcc ggcagtggat gtaacgattc cgttcagcga
       61 gataaagctt tattcggccg attactatac cggcacagcc ggcggctccg tggcaggatc
      121 ccgcagcaaa tgggccaggc tcgccgccgc tgctcaggct acatcaacgg ctgccgggaa
      181 ggcagcgtca ataagcacac cgggaagcag gttgggcgga accttttcgc agcaggaaac
      241 agtaaacagg ctgaatgagt tcaggaacag tctcactgtt taccgcggcc ccattgaatt
      301 cataactttc actgaaccgt catccggcag cactgctgtc tcagaggaaa gcacagacac
      361 ccgggcagca gcagcccggg ctgccgttgc ccgcggagcc gtggcgggga aagccgccgc
      421 acaggttccg gcacggactg cagcagtgtt tccggctgaa aaactcctgc cgcagcgggg
      481 caatgtggat tccccgctgg gattattcag tgtgggcgga ctgacatttg aacccgacca
      541 gaaagtgaag gcacgtatag aggtaaaggg tttcactgtt gaatcagaat ggatagaaac
      601 ggaaggcatt ctggtatatc ccatagagca tgagggtttc aggtcgtcat acaagccggg
      661 cagtgagacc ttctcggctg acaacagttt tgtggtggta agtgcaatac atggcgaaac
      721 tgcaccctcg ggcaacgtga agatcctgag tggttcagac gccccgcatt catcactgac
      781 agcaccccag gccgtaccgg aattccctca ggcagtcagg gcaaaggtct ggttcagcaa
      841 ggacgttccg cttcagcccc tggaaggcca cccgggcagt gcaatagcat ctaccggacc
      901 atggagcgtc acctatgcct actcatcacc gcttgacgcc atcaatccga ggaagaacgg
      961 caagcatccc ttcgagatgg tcacatatgt atttaagggg aaggaccatg gttattccga
     1021 ctatgccgga gagtgtatct cctgcacttc accggtcaat attatcgaaa agatgggaag
     1081 ggatgccatg ccggggactt ccggccggac cggaaggccg gcgatgcagc aaaatgcaaa
     1141 tgttccccgg gaagcaccgc agaaacagat caaaaagcct gtgatggtga gatagtgtga
     1201 atcccggact gccatgaccc ggaagcaggc ccttatacaa gcccggttca gggatgaccg
     1261 gggccggagc gggaacagga ttttattgct acatttgccc tttccctgaa ccatggagac
     1321 caggatcatc atatctcaga ttttcatgct cgctgttgtt gtgcttatcg gggcaataag
     1381 cgcccgtttc agggtaatca caaccgaaat aaaggatatg ttgtcaaaac tgattttcaa
     1441 catatcactt cccctgatgc tttttaccaa tttcctcagg attgaggcca caccgaagct
     1501 gatagcaaac agcctgaccg ttctggggat atccggcttt gtgatactgc ttctgttcat
     1561 catcggatgg attaccaccc gcttaatgag attcactacc tctgagtcgg cagttttcag
     1621 agctcattcc atgttcggaa atactatttt cctgggcttc ccgcttattc atgcgcttta
     1681 cggagctgag ggcctgctgt atgcaacaat gttccagctt gtttccaata taattatgtg
     1741 gacagccggt gttgtcgtgc tcactcacgg aaatggcgtg tcgtggaaaa aaagcattct
     1801 gaaggtgatg aatccgaata cgattgccac actggcaggc ctggtcttct ttatgttttc
     1861 cctgaaactg cctgaaataa tagtcaggcc cctggcagaa cttggctcag caaacacgtg
     1921 gctgtcaatg ttgtatatcg gggcaatgct tgtttttgca aatgttggcg atcttcttaa
     1981 aacaaaaagt ctttatatca taagtttcaa caggcttatc ctggctccgg caatccttgt
     2041 tgctgtcttt tacgttttcg gcgtatttgc tggtctggct ccggacaaac tggtaacctc
     2101 ggttataatt ctggaagcat ccatgccgtg catggcaaca gtggttatca tggcaaagga
     2161 attcggggca gatgaccatc ttgccgtggg taacgtattt gtttcaacta ttctgagcat
     2221 tgttaccctg ccgctggtgc tgatggcgat taacagtttc ctgtaggatt tccgaggtat
     2281 ttgagaataa aatgtcatct ccggcattaa acaaataatt cttccgggaa acatgtgtcc
     2341 ggcagaaatg gaatatgccg gtacaggaaa gccgattaat ttcagactcc tgttgacatt
     2401 tgtcgtaaga gggcttaaat ttgatgcagc gataatgttc tgttttaaaa ccggcaaaca
     2461 actttgatgt aagaaatgat attgttccgc ttcacaaaca actaaacaac aagagagaaa
     2521 tgccgcgatt tgattttgaa aaactgaatg caaaccttga gatccagggt ttcgatgatt
     2581 cccttaccag ggaaaagata atgaatgcca tccgggcagt accggagaag acatacgaca
     2641 aggctgttgt ttttcttggc atagcaaccc ttatcgtagc acttggggga ataatccttg
     2701 cagccatggg caaaacggtc cctgatgccc tgtgggcagc cctgggagcc ggtatcggcg
     2761 gtcttgcagg aatctttacc ggaaagtgat aaaagattcc tgcagcaggg gaggattttt
     2821 ccgggtcaga gtccggatgc aaaattcata agaccacagt atgtcgctta tgcattcgga
     2881 aaaatcactg aagcattgag ttgaacagca gcttgaaagt tccctgtgtt gaagctctga
     2941 actgggggct gaaggcaaaa aggatgagtt ctccctttcc tttcctgatc catatcattg
     3001 ccggcttttc cgaaattatc tcctcctttt cgctgtagcc gcttacaagg atatcttttt
     3061 ccggaaatgc acctatcacc ctgcggtcca tgtcaaaacg cggcatgctt gtattgaaca
     3121 gcgggttgcc gcggtaaaaa atgtttgttt ccccgggcat gccccatgtg agtggatgat
     3181 ctttccggag tatcatccgt acaagggacc cgggaatatt cagtccgtca ttgcgggccc
     3241 gggttccagt gtttgtgaag ggaagcatga attcctcctt ttcttctcct ttgctc
//
LOCUS       DAOSPX010000345         3284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_26275_length_3284_cov_4.797770, whole genome shotgun sequence.
ACCESSION   DAOSPX010000345 DAOSPX010000000
VERSION     DAOSPX010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26275_length_3284_cov_4.797770"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLZ75_12860"
     CDS             complement(<1..369)
                     /locus_tag="PLZ75_12860"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS73327.1"
                     /translation="MAGIYLHIPFCRQQCHYCDFYRVVSHGDNSLFIDALKKEMIIRK
                     DYLAGSNVSTVYFGGGTPSLLKAEETASILDHIRNLFRVDSEAEITLEVNPDDVSHKY
                     MKDLHRAGVNRISIGIQSFRD"
     gene            473..853
                     /locus_tag="PLZ75_12865"
     CDS             473..853
                     /locus_tag="PLZ75_12865"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOS73328.1"
                     /translation="MKKNIYTITVSLIILYLSLATPKSIIRTGVFDIPHLDKLAHFGL
                     YFLLMFTMILEHRKSLTDTRKLLLISLIPLFFGITIEFIQSFFTRNRTGEVLDAIADA
                     GGIAAAVLLWIFFRPYHHPEEEIR"
     gene            complement(846..1286)
                     /locus_tag="PLZ75_12870"
     CDS             complement(846..1286)
                     /locus_tag="PLZ75_12870"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOS73329.1"
                     /translation="MLSNSSRYGIRAVTYLASRPDRKVQTGIATISGDLGLPAPFLAK
                     ILQQLVRHKILNSSKGPHGGFSLSKDPGEITVYEIVRIIEGEDIFHNCIIHNLSCREV
                     HERGEECILHSDYSLIRRNLVDLFRSRTIDDLLRQAEGSDRIVI"
     gene            complement(1288..1734)
                     /gene="rpiB"
                     /locus_tag="PLZ75_12875"
     CDS             complement(1288..1734)
                     /gene="rpiB"
                     /locus_tag="PLZ75_12875"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOS73330.1"
                     /translation="MKKKKIAIVSDHAGFYLKGRLLDHLKKEKYEVRDFGSYSFDSVD
                     YPDFGHKMAESVSAGEYDVGISICGTGNGINMTANKHQGIRSALCWNEEISRLARAHN
                     DANVCALPGRFISESEAYLIVKTFLETGFEGGRHKRRIDKIPLKTD"
     gene            2161..3111
                     /gene="nadA"
                     /locus_tag="PLZ75_12880"
     CDS             2161..3111
                     /gene="nadA"
                     /locus_tag="PLZ75_12880"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOS73331.1"
                     /translation="MQNAEYIAAINRMKQEKNAVILAHYYQTPDIQDIADFVGDSLAL
                     AQKAACNEAEIILFAGVKFMAETAKIISPGKKVLLPDMSAGCSLADSCSAEDLKKFIA
                     ANPGRTLIAYINSTTEIKAMADIICTSSNVIRIIESLPENERIIFVPDRNLGSYAREK
                     TGRDIIIWDGACHVHENFSAEGIIRLRDVYPGARVLVHPECEYPVRQLADFTGSTAGM
                     LEFVERDKNKTYIVATEPGVIHQMKKINPEGKYIAAPAKISLTSGNECNYMKLITIEK
                     IYNCLKNEEPFIELDSELMAAARKPVERMFDISKIPAGSR"
BASE COUNT         1000 a          665 c          643 g          976 t
ORIGIN      
        1 atccctgaac gactggattc ctatgctgat cctgttgaca ccggccctgt gcagatcctt
       61 catatattta tggctcacgt cgtcgggatt gacttcaagg gtaatttccg cttcactgtc
      121 caccctgaac agattcctga tatgatccag tatggatgcc gtttcttcag ctttcagcag
      181 ggagggtgtt cctcctccga aataaacggt tgaaacattg ctgcccgcca gataatcctt
      241 tcttattatc atttccttct tcagggcatc aataaaaagg gaattgtcac catgcgaaac
      301 tacacggtaa aagtcacagt aatgacactg ctgcctgcaa aaaggtatgt gtagatatat
      361 tccagccatt ggtaaaaaat actttcacag atacaaagaa acacaataga tcagaatttt
      421 ctatgtttta aaaggactaa ttgatacatt tgccacaaaa tttcccggtt tgttgaaaaa
      481 aaacatatac acgataaccg tttcactgat tatcttatat ctgagtctcg ccacaccaaa
      541 gtcgattatc aggaccggtg tttttgacat accccatctg gacaaacttg ctcattttgg
      601 tctttatttc ctgcttatgt tcacaatgat ccttgaacac agaaaatcac tgaccgatac
      661 aaggaaactg ttgctgatat cgcttattcc gctttttttc ggaatcacta ttgaatttat
      721 ccagtcgttt ttcaccagga accgtacggg tgaagtgctg gacgcaatag cagacgcagg
      781 cggtattgca gctgccgtat tactctggat ctttttccgg ccctatcatc acccggagga
      841 agaaatcaga taactatcct gtctgatccc tcagcctgcc tgagaaggtc atctatggtt
      901 ctgctcctga aaagatcaac aagattccgg cgtatcagcg aataatcgct gtgcagtata
      961 cattcctccc ctctttcatg tacctccctg cagcttaaat tatgaattat gcaattgtga
     1021 aaaatatcct ccccttctat tatcctgaca atttcataaa cagttatctc cccgggatcc
     1081 tttgaaagtg aaaagcctcc gtgtggtccc tttgatgaat taagtatctt gtgccttaca
     1141 agctgctgca gtatttttgc caggaaaggg gccggaagac caagatctcc ggaaatagtt
     1201 gctattccgg tctgtacctt cctgtccggc ctgctggcaa gataggtaac tgcacgtatg
     1261 ccatatcttg atgaatttga aagcatctca gtctgttttt aaaggtattt tgtcaatccg
     1321 ccttttatgc cttcctcctt caaaaccggt ttcaagaaaa gttttaacaa taagatatgc
     1381 ttccgattct gaaataaatc ttccgggcaa tgcacataca ttggcatcat tgtgagctct
     1441 tgcaagccgg ctgatctctt cgttccagca taaggcactc cttattccct gatgtttgtt
     1501 ggcagtcatg ttgatgccat tgccggttcc gcagatggat attccgacat catattcacc
     1561 agctgaaacg gattctgcca ttttatgacc aaagtcagga tagtcgacac tgtcaaagga
     1621 ataggatccg aaatccctga cttcatattt ctcttttttc aaatgatcca gtagtctccc
     1681 tttcaggtaa aaaccggcat gatcagaaac aattgcaatc tttttctttt tcacattttt
     1741 taagtttact gtaactgttg ataagctgta gaaaagttat aaaaaaacac tattcccggt
     1801 ttaatttact gcaaaagcat caaatttcat ctctttccac aaaaacaaaa atacttttca
     1861 taaaataata tcaggaaata aaacataaat gaacattaaa aatataaatt tgtttcatca
     1921 gatctgaata aatgtgatta ttaacaactg ttggttataa aactaaaata ctgtgtatca
     1981 aaaatggtat attttttgag ttgttgataa aatgtttgca ttatggcata aatgaaaaat
     2041 acaacagttc tcatttatta tttcgaacac aattaacagc atagtattat catcatatat
     2101 attatttaaa tttaaattaa gttatatatg ttgttaatat tgtaaaaaat aatgtggaaa
     2161 atgcagaatg cggaatatat agcggcaata aacaggatga agcaggaaaa aaatgcagtc
     2221 attcttgctc attactatca gactccggat attcaggata ttgcggattt tgtcggggat
     2281 tcgcttgcat tagcccaaaa ggcagcatgt aatgaagctg agataatatt gttcgccgga
     2341 gttaaattca tggcagaaac ggctaaaatt atatctcccg gtaaaaaggt gcttctgcct
     2401 gatatgagtg caggatgttc tcttgctgat tcctgcagtg cggaggatct gaaaaagttt
     2461 atcgccgcaa accccggaag aacccttatt gcatatatta attccacaac ggaaataaag
     2521 gcaatggcgg atataatctg cacttcgtcg aatgtgatca ggattataga atcactgcct
     2581 gaaaatgaaa gaataatttt tgttcctgac aggaatctgg gaagctatgc gcgcgaaaaa
     2641 accggacggg atataattat ctgggacggg gcatgtcatg ttcatgaaaa tttctcagca
     2701 gaaggaatta tacgcctgag ggatgtttat cccggagcca gggtacttgt tcatcctgaa
     2761 tgtgaatatc cggtcaggca gctcgctgat ttcacggggt caacggcagg tatgcttgaa
     2821 tttgtggaaa gagataaaaa taaaacctat attgtggcta cggagccggg ggttattcat
     2881 cagatgaaaa aaataaatcc ggaaggtaaa tatattgccg cccctgcgaa aatatcgctt
     2941 acttcgggaa atgagtgtaa ctatatgaaa ctaatcacaa ttgaaaaaat atacaattgt
     3001 cttaaaaatg aagagccgtt tatagagctt gactctgaac tgatggcagc tgcgcgtaaa
     3061 cctgtggaaa gaatgttcga catttctaaa atacctgccg gaagccgatg aaaagaccag
     3121 caataatcat attgtttatt ttttttgcgg ctaaggcttt ttcccagggc gatgagatcg
     3181 ttgcagacgg atatcaggta ttccgctatc ccacggggac catctcaagc gaggggtaca
     3241 tgaaaaacgg gacacccgaa ggttaccgga aaagctacta tgtt
//
LOCUS       DAOSPX010000346         3274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_26394_length_3274_cov_4.153774, whole genome shotgun sequence.
ACCESSION   DAOSPX010000346 DAOSPX010000000
VERSION     DAOSPX010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26394_length_3274_cov_4.153774"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLZ75_12885"
     CDS             <1..809
                     /locus_tag="PLZ75_12885"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOS73332.1"
                     /translation="IFTMLVLIFVLFLLVNFNTSVFFIGERTFFPAFMYILMTALLPF
                     NQVPNPVLPAIVFLMLALKRIMGSYRKAGTAFNFFDAALFISTGSLFYANLIWFGAVV
                     FIGIILLRSGNIIEITTSVIGLATPYIILAGIYYVIGKDVSLPVSVLGENLFAEPAEF
                     ALSRIETLTLVFTGLMILTAVAFLLKMIDSRKIKSRKTFYLLLWVMIITVVVYFTVPS
                     ASAELLWIFAIPVSYILSHYFIFVRKKVVPEIMFSGFFLLVLLVQVLSLL"
     gene            complement(935..1369)
                     /locus_tag="PLZ75_12890"
     CDS             complement(935..1369)
                     /locus_tag="PLZ75_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73333.1"
                     /translation="MKLYLSLIIMMITACITAYGQSSELLVSNCVQNAGPDARYLKDF
                     RIQLGKTDNPGEPRYKTQMSLWKNTKYRFSLCNSEDSEGLLYLNLKDDANKMVLSSLD
                     PETGKTYAFIDFICNKSGIYQLSFDFTGGLKGSGVGVVSMIK"
     gene            complement(1371..2285)
                     /locus_tag="PLZ75_12895"
     CDS             complement(1371..2285)
                     /locus_tag="PLZ75_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73334.1"
                     /translation="MKGNLFFILIVSLILSVSCRNSQKNSSGFVFPEDDSIPLGEAEK
                     LSAEAIADISNNIASPVEIANLLQMLSVPYSPGYLASSINANRQPTDFDKALALGILG
                     ADLGYLNMYEKTATAINILSDIKKLAEGLKVGQFFDFEAIRRLSLNRSNLDSLLFLSI
                     DSYTRIDKYLRDNGRGHLSALMIIGVWIEGQYLATQVIREYPHPVLRDRIGEQKIILN
                     DLIMLAAPYCERDTEFRALCGYLQDMREKFRNVKITYSLGDPVSIEKDGGLVVEQTET
                     SNVEMSDEQLAGLVEITGNIRNKLVLNN"
     gene            complement(2328..>3274)
                     /locus_tag="PLZ75_12900"
     CDS             complement(2328..>3274)
                     /locus_tag="PLZ75_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73335.1"
                     /translation="FNSVITIYIIIPLLIIPMMVLSGAMFPFDKLNRKIGNVGKVPFL
                     AELMPTRWTYEALMLTQFRNNRYSTTEYNREGETYYSLQKKISESDFNRVYRIPELKR
                     RLSDIPRENVPASTAGKTPARPEKWSSDLILLKNEMIKISDVYKVPQFGHIASLSPEA
                     FSSGIADSLNSYLSRLDEIFSMRFNSASETRDRFYNLNSAKLNELQDQHYNYQLEQIV
                     TKYFERHKMLIYNNSIIQNVDPVYLDPVKKGVLSFRTHFFAPTKYFLGKNIDTYRFNI
                     RVVLINSLFLYLILYFNLLARAIGSLEKFKTRKRLISK"
BASE COUNT          772 a          742 c          745 g         1015 t
ORIGIN      
        1 tgatattcac aatgctggta ctgatattcg tccttttcct gctggtcaat ttcaacacat
       61 cggtattttt catcggggaa cgtacctttt tcccggcctt catgtatatt ctcatgaccg
      121 cccttctgcc gttcaatcag gttcccaacc ccgtccttcc ggcaatagta ttcctgatgc
      181 ttgccctgaa acggataatg ggatcctacc gcaaagccgg aaccgcattc aatttttttg
      241 acgccgccct cttcatcagt accgggtcgc tgttttacgc gaatctcatc tggttcgggg
      301 cagtggtgtt catcggtatt attttattga gatctggaaa tattattgag ataacaacat
      361 ctgtaatcgg ccttgccaca ccctacatta tcctggccgg catctactac gttatcggga
      421 aggacgtcag tctgccggtt tccgtgttag gggagaatct ttttgctgag ccggctgaat
      481 ttgcactttc gagaattgaa acgcttaccc tggtctttac gggactgatg atacttacgg
      541 ctgtggcttt tctgctgaaa atgatcgaca gcaggaaaat aaaatcacgg aagactttct
      601 acctcctgct gtgggtgatg ataatcacgg ttgtggtgta tttcacagtg ccttcggctt
      661 cagcggaact gctgtggatt tttgcaatac ctgtttcata catcctgtca cactatttca
      721 ttttcgtcag gaaaaaggtt gtccctgaga tcatgttcag cggtttcttc ctgctggttt
      781 tactggttca ggttctgagc ctcctttagt tgtcttttca ttcggcgatt tccactttac
      841 cgggatttcc gccttgtaaa ccggctgttt gtgttctgcc gcaatcctgc ctgtgggccc
      901 gaccgtatta tttgccgtgt ccttaacccg gaaatcattt tatcattgat accactccga
      961 ctcccgaacc tttcagtccg ccggtgaaat cgaagcttag ctggtatatt ccgcttttgt
     1021 tgcagatgaa gtcgatgaag gcataggttt tcccggtttc gggatccagg gatgagagta
     1081 ccattttatt tgcatcatcc ttaaggttca gatacagtaa accctccgaa tcctccgagt
     1141 tgcacaggga aaacctgtat ttggtattct tccagagcga catctgggtt ttgtatcttg
     1201 gttctcccgg attgtctgtc ttcccgagct gaatcctgaa atccttgaga tatcttgcat
     1261 cgggtcctgc attctggaca cagttgctca ccaggagttc tgatgactgt ccgtatgccg
     1321 tgatgcatgc ggtgatcatc ataatgatca gtgatagata taacttcatg tcagttattt
     1381 aaaaccagtt tattgcgaat gtttccggta atctcaacca gtccggccag ctgttcatcg
     1441 ctcatttcga cattgctcgt ttctgtctgt tcaacaacaa gtccaccgtc cttttcaatg
     1501 ctgacaggat cacccaggct gtatgttatt tttacattcc tgaatttttc cctcatatcc
     1561 tgaagataac cgcacagtgc cctgaattcc gtatcccgtt cacagtacgg tgccgcaagc
     1621 attatgagat cgttgagaat aattttctgt tcacctatcc tgtcacggag aaccggatgg
     1681 ggatattccc tgatgacctg tgttgccaga tactggcctt cgatccacac tccgattatc
     1741 ataagtgcag acagatgccc ccttccgtta tcccggagat atttgtctat ccttgtatag
     1801 gagtcgatgg acaggaacag gagggaatcc aggttggacc ggttgaggga cagccttctg
     1861 attgcctcaa aatcaaaaaa ctggccgact ttcagacctt ccgcaagttt cttgatgtca
     1921 gaaagaatat tgattgcagt agctgtcttt tcatacatgt tcagataacc caggtcggcc
     1981 cccaggattc ccagggccag cgccttgtca aaatccgttg gctgcctgtt tgcatttatt
     2041 gatgaagcca gataaccggg cgaatagggt acggacaaca tctgaagaag atttgcaatc
     2101 tcaaccggtg aggcgatatt gtttgaaata tcagctatag cttcagcact taatttttca
     2161 gcctcaccca gagggattga atcatcctcg ggaaaaacaa acccggagct gtttttctgg
     2221 gaatttctgc atgagacgga taatataagt gaaacgatca gaataaaaaa taagtttcct
     2281 ttcatcagac tacattgaat aatgcaaata tataaaaaag tatttaacta tttacttatt
     2341 aatcgttttc gggttttaaa tttttcaaga gaaccgatgg cccgggcaag aaggttgaaa
     2401 tacaatataa ggtacagaaa gaggctgttg ataagtacta ccctgatatt gaacctgtaa
     2461 gtatcaatat tttttcccag gaaatatttg gtcggtgcga aaaaatgagt tctgaacgac
     2521 aatactccct ttttcaccgg atccaggtag accggatcca cgttctggat tattgaattg
     2581 ttgtagatga gcattttatg ccgttcaaag tattttgtga ctatctgttc cagctggtaa
     2641 ttatagtgct gatcctgcag ttcgttcagt ttagcagagt tcaggttata gaatctgtcg
     2701 cgtgtttcgc ttgcggagtt gaagcgcatg ctgaagattt catccaggcg gctgaggtat
     2761 gagttcagtg agtctgcaat tccgctgctg aatgcttcgg ggctgagtga agcaatatgg
     2821 ccgaattgcg gcaccttgta aacatcactt atttttatca tttcgttttt caggagaatc
     2881 aggtcgctgc tccatttttc cggcctggcc ggggttttgc ctgcggtgct ggccgggaca
     2941 ttttcccggg gaatatccga caatcttctt ttcagttccg gaatccggta aaccctgttg
     3001 aaatcggatt cgctgatttt cttctgaagg gaataatatg tttccccttc gcggttgtat
     3061 tcggtggtac tgtagcggtt gttcctgaac tgcgtgagca tgagggcctc ataggtccag
     3121 cgggtgggca tcagttccgc cagaaagggc actttcccga catttccgat tttcctgttg
     3181 agcttgtcga agggaaacat ggccccgctc agtaccatca tcggtatgat gagaaggggt
     3241 attataatat aaatggttat tacggagtta aatg
//
LOCUS       DAOSPX010000347         3267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_26477_length_3267_cov_4.319738, whole genome shotgun sequence.
ACCESSION   DAOSPX010000347 DAOSPX010000000
VERSION     DAOSPX010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26477_length_3267_cov_4.319738"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLZ75_12905"
     CDS             complement(<1..772)
                     /locus_tag="PLZ75_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOS73336.1"
                     /translation="MYNQSQLKELVDKALMNLSYNTEAPRLIDPVKYIMSLGGKRLRP
                     VMLLMACNIFSDRINDAVMPAAGIEIFHNFTLVHDDIMDQASLRRGLATIHHKWNVNQ
                     AILSGDVMAFLAGDCFLQLPQGNLVRVLRIFNKAARDVCAGQQLDMDYEKAVIVTQEE
                     YLKMIELKTAVLIAASVKIGAIIGGADEKNADLLSEFGNNLGLAFQIQDDILDVWGDS
                     KVFGKKPGGDIVANKKTLPLVKAMEKASVPQKKVLQELF"
     gene            complement(792..1679)
                     /gene="atpG"
                     /locus_tag="PLZ75_12910"
     CDS             complement(792..1679)
                     /gene="atpG"
                     /locus_tag="PLZ75_12910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOS73337.1"
                     /translation="MANLKEIRVRIASVKSTRQITSAMKMVSAAKLRRAQDKILKLRP
                     YANKLHEILVNLSQSLAETQIENVYGRSSLPGKILLVVITSNKGLCGAFNSNVIREVR
                     RLISEKYADQHRNGNVSILPVGKKGYDYFRNLNIKMLPDCNDLLNDLTFNNAVLVAES
                     VMKSFVAGEYDRVEIIYNQFKNAAVQNLTDEVFLPVETAQPDEFSAAMPADYIYEPTK
                     EEIIEELIPKSLKIQFYKAVLDSFVAEHGARMTAMHKATDNATELIRDLTLEYNKARQ
                     ASITNQILEVVSGAEALKG"
     gene            complement(1710..3260)
                     /gene="atpA"
                     /locus_tag="PLZ75_12915"
     CDS             complement(1710..3260)
                     /gene="atpA"
                     /locus_tag="PLZ75_12915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOS73338.1"
                     /translation="MLRQQLEGIQTGSDLEEVGTVLTVGDGIARIYGLSNAQANELIE
                     FENGIEAIVLNLEEDNIGAVLLGPTEEINEGDIVKRTGRIASIGVGEGLLGRVISPTG
                     QPLDGKGPVEGRLYNMPFERKAPGVIFRQPVKQPLQTGLKPIDAMIPIGRGQRELIIG
                     DRQTGKTAIAIDTIINQRSNFEKGEPVYCIYVAVGQKGSTVANIAKTLEEYGALEYTV
                     IVLATASDPAASQFYAPFAGAAIGEFFRDTGRDALVIYDDLSKQAVSYREVSLLLRRP
                     PGREAYPGDVFYLHSRLLERAAKIIESDDVARQMNDLPESIRHMVKGGGSLTALPIIE
                     TQAGDVAAYIPTNVISITDGQIFLESNLFNAGVRPAINVGISVSRVGGNAQIRAMKKV
                     AGTLKVDQAQYRELEAFSKFGSDLDAATMAVLNKGSKNVEILKQGQYRPMPVEKQIAI
                     IYCGTMALLRDVPVSKVNEFENEYLEMLDLKHRDVLDRLAEGTIDDDITKVLEKVAAE
                     VAMKYKSK"
BASE COUNT          755 a          840 c          716 g          956 t
ORIGIN      
        1 caaagagttc ctggaggact tttttctgcg gaacagaagc tttttccatt gctttcacca
       61 ggggcagggt ctttttgttt gcgacaatat cacctcccgg ctttttaccg aatacctttg
      121 aatcacccca tacatcaaga atgtcatcct gtatctggaa tgcaagaccg agattatttc
      181 cgaattcgga aagcaggtca gcattttttt cgtcagcccc gcctataatt gctccgattt
      241 ttactgatgc ggcaataagt actgctgttt tcagctcaat cattttcagg tattcttcct
      301 gtgtaacgat tactgccttt tcataatcca tatcaagctg ttgtcccgca catacgtccc
      361 tggcagcctt gttgaagatc ctcaggaccc ttaccaggtt cccctgcgga agctgaagga
      421 agcagtcacc tgccagaaaa gccatcacat ctcccgataa tatcgcctgg ttaacattcc
      481 atttatgatg aattgtcgca aggccccttc tgagtgaagc ctgatccatg atatcatcat
      541 gcaccagggt gaaattatgg aatatctcta ttccggcagc aggcatcacc gcatcattta
      601 tcctgtcaga aaagatgttg caggccatca gaagcataac gggacgcagc cttttgcctc
      661 ccagcgacat gatatattta accggatcga tcagtctggg tgcttccgta ttgtatgaca
      721 gattcatcag ggccttatcg accagctctt taagttgaga ttgattgtac attcccttaa
      781 aaagatgata actaaccttt aagtgcttcc gcaccgctga cgacctcgag tatctgattg
      841 gtaatcgatg cctgcctggc cttgttatac tcaagggtaa gatcgcgtat cagttcggtg
      901 gcattatcgg ttgccttgtg catcgctgtc atccttgccc cgtgttcagc aacgaaggaa
      961 tccagcacag ccttgtaaaa ttgtatttta agtgacttgg gaatcagctc ttctataatc
     1021 tcctcctttg tgggttcata gatgtaatca gcaggcattg ctgctgagaa ttcatcgggc
     1081 tgagcggttt ccacaggaag gaagacttcg tcggtcagat tctggacagc agcattcttg
     1141 aactggttgt aaataatctc aaccctgtca tattccccgg caacgaaact tttcataacc
     1201 gattcggcaa caagcacggc attgttgaaa gtaaggtcat taagcagatc attacagtct
     1261 ggcagcatct tgatattcag gttcctgaaa taatcatagc cctttttccc gacgggaaga
     1321 atgcttacat ttccattccg gtgctgatct gcatatttct cagatatcag ccttcttact
     1381 tccctgatca catttgaatt aaaggctccg cataatccct tgttagaagt tattaccacc
     1441 aggagtatct tcccgggcag ggagcttctg ccatatacat tctcaatctg tgtttccgcc
     1501 aggctctggg acaggttgac aagtatctca tgcagcttgt ttgcgtatgg ccggagcttc
     1561 agtatcttgt cctgtgccct tctcagtttg gcagcagaaa ccattttcat ggctgatgtt
     1621 atctgccttg tcgattttac cgaagctatt ctgaccctta tttcctttaa attcgccatt
     1681 ctgaaattat attttggccg ctctcccttc tattttgatt tgtatttcat tgctacttct
     1741 gccgcaacct tttcaagcac ttttgtaata tcatcatcaa tggttccttc cgcaagcctg
     1801 tcaagaacat cacggtgctt cagatcaagc atctccaggt attcattctc aaactcattc
     1861 acttttgaaa ccggaacatc cctgagcagg gccatggttc cgcaatagat tatagcaatc
     1921 tgtttctcca ccggcatggg tctgtactgt ccctgtttaa gtatttcaac gttctttgaa
     1981 cccttgttca gaactgccat ggtggctgca tcaaggtcgg aaccgaattt tgagaatgcc
     2041 tcgagttccc tgtactgggc ctggtctact ttcaatgtcc cggcaacctt cttcatggcc
     2101 cggatctggg catttccacc caccctcgaa acggagattc cgacattgat agccggcctg
     2161 actccggcat tgaagaggtt ggattccaga aatatctgcc cgtcagtgat ggatatcaca
     2221 tttgtgggta tatatgcggc aacgtcaccg gcctgggttt ctataatggg cagggcagtg
     2281 agggaacctc ctccttttac catgtgccgg atcgattctg gaaggtcgtt catctgccgg
     2341 gcaacatcat ccgattcaat gattttcgcc gctctttcaa gcagccgtga atgaaggtag
     2401 aagacatctc ccgggtatgc ttcacgtccg gggggtcggc gcaggagcag ggaaacctcc
     2461 ctgtatgaga ctgcctgttt cgaaaggtcg tcatagataa cgagtgcatc ccttccggta
     2521 tcgcggaaaa attccccgat ggcagcacct gcaaagggtg cgtagaactg ggatgcagcg
     2581 ggatcggaag ctgttgcgag aacaatcacg gtatattcaa gagccccgta ttcctcaagc
     2641 gttttggcaa tattggcaac tgttgatcct ttctgtccca cggcaacata gatgcagtaa
     2701 accggttcac ccttctcaaa attgctccgc tggtttatga tggtgtcaat ggcaattgcc
     2761 gttttcccgg tctggcggtc accgattata agttccctct gccctcttcc tatgggtatc
     2821 attgcatcaa tgggcttcag tccggtctga aggggctgct ttacgggctg acggaaaata
     2881 actcccgggg cttttctctc gaagggcatg ttatacagcc tgccttcaac agggcccttc
     2941 ccgtcaagcg gctggccggt cggggatata acccttccga gcagtccttc accgactccg
     3001 atggatgcaa tacgtcctgt ccgctttaca atgtcccctt catttatttc ttccgtcgga
     3061 ccaagaagca ctgcaccgat attgtcctcc tccaggttaa gcacgatggc ttcgatgccg
     3121 ttttcaaact caatcagttc atttgcctgt gcatttgaaa gaccgtaaat acgggcaatt
     3181 ccgtcaccaa cagtaagaac tgtacctact tcctccaggt ctgatccggt ttgaattccc
     3241 tcaagttgct gtcgcagaat gctttat
//
LOCUS       DAOSPX010000348         3261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_26542_length_3261_cov_4.174049, whole genome shotgun sequence.
ACCESSION   DAOSPX010000348 DAOSPX010000000
VERSION     DAOSPX010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26542_length_3261_cov_4.174049"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLZ75_12920"
     CDS             <1..363
                     /locus_tag="PLZ75_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73339.1"
                     /translation="SKPEIREHARRAGISVWEKPALSCLLTRIPYNTEISSDILSMVE
                     KAEDFLSEKGFPGTRVRIHGDVARIECIPDHMEKFVRDPDRKSIVGYLKEIGFRYVSL
                     DLEGYRTGSMDHPYNSEK"
     gene            360..1109
                     /gene="larB"
                     /locus_tag="PLZ75_12925"
     CDS             360..1109
                     /gene="larB"
                     /locus_tag="PLZ75_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOS73340.1"
                     /translation="MTGKEIEKLLTDVRDGSKSVSEALEILKSFPYTDLGFARIDHHR
                     EIRTGYPEIVYCAGKTTVQVREIFRIMSEKENNIVGTRADSAMYEAVREICPDARYYE
                     EARIISIQKQKPEMPESRIAVITAGTSDIPVAEEAAVTAELLGNRVVRIYDAGVAGIH
                     RLVDKLPEIRSCKVIIVIAGMEGALASVVGGLVDKPVIAVPTSVGYGASFGGISALLA
                     MLTSCSAGVTVVNIDNGFGAGFSASMINRMT"
     gene            1122..1727
                     /locus_tag="PLZ75_12930"
     CDS             1122..1727
                     /locus_tag="PLZ75_12930"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOS73341.1"
                     /translation="MPGNVFVTSAYLPSAEYFSKIIRAEKIFVEAEENYRKQSYRNRC
                     YILASQGPEHLVVPVFLGSFHRTHIRDIRIDYTRRWQKVHTGALAAAYGSSAFFIYYY
                     GIIEEIILRRYKFLLDLNMDLLTALLGILNIDVKPEYTETFMPAGNAEGDFRYSIDPK
                     KKSSYIQKEYFQVFRDRHGFVPGLSIVDLIFNMGPETPGYL"
     gene            complement(1778..>3261)
                     /locus_tag="PLZ75_12935"
     CDS             complement(1778..>3261)
                     /locus_tag="PLZ75_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73342.1"
                     /translation="KFRLILIIILSSTAVLHAQNFDDALRYSRVFYGGTARFLSMGGA
                     FTALGGDISTLGQNPAGIGLFRSSEISVTPQLYHIKSTSDFNGLTSDFLYNFNLNQAG
                     LVVNLIRKNAESGLVSLNFGYTYSMKSNLNQNGLIRGISNRGSMADYWADISTGLFKK
                     ELYKYAADADLAYWAGLIDTLPGYSREYGSIFEYYGESDVADYGQTVRRLISDEGSTS
                     DHSFSIGGNYSNRLYFGLTFGVSTINYISHYEHLETTGNIAPYGLDNFNYALHYENSG
                     RGFSAKLGVILKPVEMLRIGLGIHTPTLYRINEYIHDNITSAYLDGDKFDFKNDPSRY
                     SYALTTPVRFLAGVALQIGKIGLVSADYELVDYSSSKFSETGDGYDYSQKNEAIGKNL
                     RRSNNIRLGAEMRYNHLYFRGGYGYYGKAFSQNDINRALDYQIFSVGTGFREQNIFVD
                     LAFSRMINSQKYILFQSSAGDAISHMSIGRNMFTLTLGYRFGY"
BASE COUNT          926 a          730 c          773 g          832 t
ORIGIN      
        1 tcgaaaccgg aaataaggga gcatgcacgc agggcgggca tttccgtatg ggagaaaccg
       61 gcactgtcat gccttcttac caggattccc tataacacgg agataagcag tgatattctt
      121 tcgatggttg aaaaagctga agattttctt tctgaaaaag gatttcccgg cacaagagta
      181 aggatacatg gtgatgttgc aagaatagaa tgcattccgg accacatgga aaagtttgta
      241 agggatccgg acagaaaatc catagtcggc tacctgaagg aaatcggttt cagatatgta
      301 tctttggacc tcgaaggata cagaacaggc agcatggacc acccatataa ttccgagaaa
      361 tgactggaaa agaaattgag aagcttctca ccgatgtcag ggacggatca aaaagcgttt
      421 ctgaagctct tgaaatactt aaatcattcc cctataccga cctgggattt gccaggatcg
      481 atcaccacag ggagatccgg acgggatatc cggagatagt gtattgtgcc ggtaaaacca
      541 cggtacaggt aagggaaatc ttcaggataa tgtcggaaaa agaaaacaat atagtaggca
      601 caagggccga ttcagcaatg tacgaagccg tcagggaaat ctgtccggat gcccgttatt
      661 atgaagaggc acggatcata tccattcaga agcaaaagcc ggaaatgccg gaatcccgca
      721 tcgctgtcat caccgcgggg acatccgata ttcccgtggc cgaagaagct gccgtcactg
      781 cggaactcct cggcaacagg gttgtcagaa tctacgatgc aggcgttgca ggaattcaca
      841 gacttgttga caaactgccc gagataagaa gctgcaaggt aataattgtc attgcaggaa
      901 tggaaggtgc ccttgcaagt gttgtgggag gacttgtcga caaacctgta atagctgttc
      961 ccacaagcgt cggatacgga gccagtttcg gaggcatctc tgccctcctg gccatgctta
     1021 caagctgctc tgccggagta acggtggtaa atatcgacaa cgggttcggt gccggattct
     1081 cggcaagtat gataaacagg atgacatgac tgcccccgat catgcccgga aatgtttttg
     1141 taacaagtgc atatctgccc tctgctgaat acttttccaa aatcatcaga gctgaaaaaa
     1201 tatttgtcga agcagaagaa aactaccgga agcagtcata ccggaaccgc tgctacatac
     1261 ttgcatcaca gggaccggaa cacctggttg tgcctgtctt cctgggaagc ttccacagga
     1321 cgcatatcag ggatatcagg atagattata cccgcagatg gcaaaaagtt cacaccggag
     1381 cacttgcagc ggcttacggt tcatcagcct tctttattta ctattacgga atcattgaag
     1441 aaatcatact ccgcaggtat aaattcctgc tggatctgaa catggatctt ctgacagctc
     1501 tgcttggaat actgaatata gatgtaaaac cggaatatac tgaaacattt atgccggccg
     1561 ggaatgctga aggagatttc agatattcca ttgatccgaa aaagaaaagc tcctacatcc
     1621 agaaggaata ttttcaggtt ttcagggaca ggcacggatt tgtccccgga ctgagtatag
     1681 ttgaccttat ttttaacatg ggtccggaaa cgcccggcta tctgtaaaga aggcctccgc
     1741 tgcgggaggc cttcaaccaa ggaagtcaaa caaataatca gtaaccgaac ctgtagccta
     1801 aagtaagagt aaacatattc ctgccaatgc tcatatgtga tattgcatcc ccggcggaac
     1861 tctggaaaag aatgtacttc tgtgagttga tcatccggga aaaagccagg tccacaaata
     1921 tattctgctc ccggaaacct gttcccacgg agaatatctg gtaatcaagc gccctgttta
     1981 tatcgttctg cgaaaatgcc ttgccgtaat atccgtatcc gcctctgaag taaaggtgat
     2041 tgtatctcat ttcagcaccc agacggatat tgtttgaacg ccgcagattt tttcctattg
     2101 cctcattctt ctgactgtaa tcgtatccgt caccggtttc ggaaaatttt gaggaagaat
     2161 aatcaacaag ttcataatcg gcacttacca atccgatctt gcctatctgc aatgccacac
     2221 ctgcaaggaa tctgaccggt gttgtaaggg catagctgta cctggagggg tcattcttga
     2281 aatcaaactt atccccgtca aggtatgccg atgttatatt gtcgtgtatg tattcattta
     2341 tcctgtacaa tgtaggggta tgtatgccca gtcctatacg gagcatttcg acaggcttta
     2401 aaatcacacc aagttttgcc gaaaaacccc ggccgctgtt ttcataatga agggcatagt
     2461 tgaaattatc cagcccgtag ggagctatgt tccctgtggt ttcaagatgt tcataatggc
     2521 tgatgtagtt tatggtggaa acaccgaaag tcagtccgaa atataacctg tttgaatagt
     2581 tgcctcctat ggaaaatgaa tgatccgaag tgcttccttc gtctgagatc agtcttctta
     2641 ctgtctgtcc gtaatccgcc acgtcactct cgccgtaata ttcgaaaatt gaaccgtatt
     2701 ctcttgaata tccgggcagg gtgtctatca gacctgccca gtaggccaga tctgcatcag
     2761 cggcgtattt ataaagctcc ttcttgaaca gaccggtact gatatctgcc cagtagtcag
     2821 ccatggaacc cctgttgctt attcccctga taagcccgtt ctggttcagg ttgcttttca
     2881 tggagtatgt atatccgaaa tttaggctga caagacctga ttcggcattt tttctgatga
     2941 ggttcacaac cagcccggcc tgattgagat tgaaattata aagaaagtct gaagtgaggc
     3001 cgttgaaatc ggaagtgctt tttatatgat ataattgtgg agtaacggaa atctccgatg
     3061 aacggaacag tccgattccg gcagggttct ggcccagtgt cgagatatca ccgccaaggg
     3121 cggtaaaagc gcctcccatt gacaggaatc ttgcggttcc gccataaaaa acccgggagt
     3181 atcttaaggc atcatcaaag ttctgtgcat gaagcacggc tgttgaagac agaataatga
     3241 ttagtatcag tctgaacttt t
//
LOCUS       DAOSPX010000349         3248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_26687_length_3248_cov_3.509239, whole genome shotgun sequence.
ACCESSION   DAOSPX010000349 DAOSPX010000000
VERSION     DAOSPX010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26687_length_3248_cov_3.509239"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="PLZ75_12940"
     CDS             complement(<1..1179)
                     /locus_tag="PLZ75_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS73343.1"
                     /translation="MKYIGKIAGLGAAALLCTNAWAQQSQKSPNILWISTEDMGPHLG
                     CYGDETARTPNLDRLASQGVRYTNVFTTAAISAPVRAGIITGMYQTSIGCMHMRTTSY
                     RRSAENPLQFTAVPPHYVKTFTEYMRTAGYYCTNNAKTDYQFAGADVPASIWDECSTT
                     AHYRNRPDKSQPFFAVFNWTGTHESQNWDISKVKTDPEKIKVPPYYPDTEIVRKNLAK
                     MYDNVARLDSVAGTLLNELEAEGELGNTIIFFWGDHGDGLPRCKRWLYDSGLNIPLII
                     KWPDNRNAGTVDDRLISSIDFGPTVLSLAGIPVPAHMQGIPFLGSRAGVARDAVFAAR
                     DRVDESYDMIRSVRTKDYLYIRNYYPNEPFPIWIPYKSRMPIYQEMLRLDAEDRLSGP
                     Q"
     gene            complement(1277..2749)
                     /locus_tag="PLZ75_12945"
     CDS             complement(1277..2749)
                     /locus_tag="PLZ75_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS73344.1"
                     /translation="MMNLKSIAGIGAATLATLPMQAQKALPPEAATPNIILILMDDMG
                     YGDLGVTGASQYDTPNIDRLARDGMQFTWFYSPQAVSSASRAGLLTGCYPNRIGITGA
                     LMPWAVHGINPEETTIAEMLGTKGYHTGAIGKWHLGHHRKFLPLQHGFDYYYGIPYSN
                     DMWPVDFDGVPIHLKDTTSRLMKYPPLPLIEGNEKIYEIGNLDGQDRLTTAYTEKAVS
                     FIEKNRNKPFFLYMPHSMVHIPLGVSEKFRGKSRQGLYGDVMMEVDWSVGEILNALEK
                     HELEKNTLVIFMSDNGPWLNFGNHAGTTGGLREGKGCSWEGGQRVPCIMKWPGKIPEG
                     TICNKLASGIDILPTLAEITGAALPERKIDGISILPLMLGDRDAMPRTEFYYYYQENS
                     LEAVQKDYWKLVFPHSGRTYRGFRPGNDGWPGQTGTEKIEKTELYDLRRDPGEWYDVA
                     EAYPEKVRELEALAEKARQDLGDDLRKIPGKNRRKPGYQD"
     gene            complement(2746..>3248)
                     /locus_tag="PLZ75_12950"
     CDS             complement(2746..>3248)
                     /locus_tag="PLZ75_12950"
                     /inference="COORDINATES: protein motif:HMM:NF027702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS73345.1"
                     /translation="DHKPAGPLRGGKYSAFDGGTRIPFIIYWPEKIRPGTTDALFSQI
                     DLMASFASLTGQELSSGTGPDSFNSLAALTGKTKKGREWLVEHSLNGRLSLIKDDWKY
                     IEPGPGPKIASYTNIELGNDTIPQLYNLKADIGEKVNVAADNESKVKELSTLLSSIKE
                     GTIKSK"
BASE COUNT          698 a          871 c          739 g          940 t
ORIGIN      
        1 ctgcggacca ctaagcctgt cttccgcgtc aagcctcagc atctcctggt atatgggcat
       61 cctgctcttg tagggaatcc agatcggaaa gggttcattc gggtaatagt tgcgtatata
      121 caaatagtcc tttgtcctta ccgatcttat catgtcatat gattcatcca ccctgtcgcg
      181 ggcggcaaac acggcatcac gggccacgcc tgcccggctt cccagaaagg gaatcccctg
      241 catatgggca ggaacgggaa ttccggcaag ggacagtacc gttggcccga aatcaatgga
      301 actgatcagc ctgtcatcaa cagttccggc attcctgtta tcgggccatt ttattatcag
      361 gggaatattt agccccgaat catataacca ccttttgcat ctcggcaatc cgtcaccatg
      421 gtctccccag aaaaaaataa tggtattgcc gagttcccct tccgcttcaa gttcattcag
      481 caaagtaccg gcaacggaat caagccttgc aacattgtca tacatttttg caaggttctt
      541 tctgacaatc tcggtatctg gataataagg cggaactttt attttttccg gatctgtctt
      601 tactttactg atgtcccagt tctggctttc atgtgtgccc gtccagttaa ataccgcaaa
      661 aaaaggctga ctcttgtccg gtctgttcct gtaatgagcc gtggtgctgc attcatccca
      721 tatgcttgca ggaacatcag ctccggcaaa ctggtaatca gtcttggcat tattggtgca
      781 gtaatatccc gcagtccgca tgtattccgt aaatgttttc acatagtgcg gaggcactgc
      841 agtgaactgc aggggattct ccgccgatct cctgtatgat gtcgtcctca tgtgcatgca
      901 tccgatcgat gtctgataca tgccggttat gattccggcc cgtaccggag cgcttattgc
      961 ggcagtggtg aaaacattgg tatatcttac tccctgcgag gccagtctgt caagatttgg
     1021 cgtccgggct gtctcatccc cgtagcagcc cagatgaggt cccatatcct cagtggatat
     1081 ccacagtata ttcggacttt tctgactctg ctgagcccag gcatttgtgc ataacagggc
     1141 tgcggctcca agtccggcaa tctttccgat atatttcata gcttgtcaaa atgatttaca
     1201 catatggcca gtcctgcatg gtcatgattt cctgaaaaca gatcacattc tatctccgac
     1261 cggttccagg gcccggttaa tcctgatatc cgggcttgcg gcggtttttc cccgggatct
     1321 tcctcagatc atccccaaga tcctgacgtg ctttttcagc cagggcctcc agttccctta
     1381 ccttctcagg atatgcttca gccacgtcat accattctcc gggatcacgt cgcagatcat
     1441 acaattctgt tttttcaatt ttctccgttc ctgtctgtcc cggccagccg tcattacccg
     1501 gcctgaatcc gcggtaggtt cttccgctgt gggggaaaac aagcttccag taatctttct
     1561 gaactgcttc caggctgttt tcctggtaat aataatagaa ttccgtgcgg ggcatggcat
     1621 ccctgtcacc cagcatcagc ggcagtatgc ttattccgtc tattttcctc tccggaaggg
     1681 ccgctccggt aatttccgcc agtgtaggaa ggatatctat tccggaagca agcttgttgc
     1741 agattgttcc ttcaggaatt tttcccggcc atttcattat acagggaaca cgctgacctc
     1801 cctcccagct gcatcccttg ccttcacgaa ggccacccgt cgttcccgcg tgatttccga
     1861 aattcagcca gggaccgttg tcgctcatga aaataacaag tgtgttcttt tcgagttcgt
     1921 gtttttcaag tgcattcaga atctcgccca ccgaccagtc gacttccatc attacatcgc
     1981 cgtataatcc ctgcctgctt ttcccccgga atttttcgga taccccgaga ggaatgtgaa
     2041 ccattgaatg gggcatatag aggaagaacg gcttgttccg gtttttttca atgaagctga
     2101 ctgccttttc cgtataagcg gttgtgagcc ggtcctggcc gtcaagattc ccgatttcat
     2161 atattttttc atttccctca attagcggca gcggcggata tttcatcagc ctgcttgttg
     2221 tatccttcag atgaatgggc actccgtcaa aatcaacggg ccacatgtca tttgaatagg
     2281 gaattccgta atagtaatcg aatccatgct gaaggggaag gaatttccgg tgatgcccca
     2341 gatgccattt cccgatggca ccggtatgat aaccctttgt tccgagcatt tcggctattg
     2401 tcgtttcctc aggatttatc ccatgaacag cccatggcat aagtgccccg gttattccta
     2461 tacggttggg atagcagcct gtaagcagtc cggcacgcga agcactgctc actgcctgtg
     2521 gactgtaaaa ccaggtaaac tgcattccgt cccttgccag tctgtcaata ttgggtgtat
     2581 catactggct ggccccggtc acacctaaat caccgtatcc catgtcatcc atgagtatga
     2641 gtattatgtt cggcgtggca gcttccgggg gaagtgcctt ctgagcctgc attggcagag
     2701 ttgccagtgt ggctgcgcct attcctgcta tagatttcag gttcatcatt tgcttttaat
     2761 tgtgccctcc ttgatactgc tgagcagtgt gctcaattcc tttaccttgc tttcattgtc
     2821 agccgcaaca ttgacttttt cgccaatatc cgctttgaga ttgtataact gcggaatggt
     2881 atcatttccc agttctatat tggtataact tgcaatcttc ggaccgggtc cgggttcaat
     2941 atatttccag tcatccttta tcagtgacaa tctgccgtta agggaatgct ccaccagcca
     3001 ttcacgtcct tttttcgtct ttcccgtcag ggcagccaga ctgttgaaac tgtcaggtcc
     3061 cgtgccggag ctcagttcct ggcctgtaag tgatgcgaag gatgccatca ggtcaatctg
     3121 cgaaaagagg gcatctgttg tacccggtct gattttctcc ggccagtata taataaaagg
     3181 tatacgtgtg cccccgtcga atgcactgta tttcccgcct ctcagcggac cggcgggctt
     3241 atggtcac
//
LOCUS       DAOSPX010000350         3225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_26941_length_3225_cov_3.197161, whole genome shotgun sequence.
ACCESSION   DAOSPX010000350 DAOSPX010000000
VERSION     DAOSPX010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26941_length_3225_cov_3.197161"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1993)
                     /locus_tag="PLZ75_12955"
     CDS             complement(<1..1993)
                     /locus_tag="PLZ75_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOS73346.1"
                     /translation="MKSNFPKGVFIIIQIFFLLSVSQLAAQMPGRNPGQAAVTGWLDD
                     SHYLFRTWDSEGKAIIQSVDIRTGKGTVISLPETSRDLLEKSLPEGTQPKPDDIVSPD
                     NSGLIRIIDNDLFYFRTGDKEFRRLTDDNAPEVNVRFCDDGSKIAYTRNRDLYVYDLV
                     SFKETRLTHDASDRIYNGYASWVYMEEILGRASRFAAFWWSPDGSRIAYLRTDESDVP
                     MFTLNRLDEEDGIHGTLEQVPYPKAGDPNPRVRMGIADLATGETVWVKTDYSADQYIA
                     WPFWTPDSRHLAVQILNRDQNNLSIILADPLTGDYREIYNEWRRTWVEFMEDIYVMKN
                     GSGFIIRSFMNDWENLYFYAWEGRLIAQLTDFDFRVTSVIRVDEDQKTVYFTATGPES
                     TDLHAWRVGLDGKNLVRLTTGEGSHNISISPKGTYYIDTWNSLSDPGSIIAYNRKGRM
                     IREIHRYEIPSFDPGRHARSEMIRIPTSDGLFNMPAIITYPLNFDPAEKYPVLFRIYG
                     GPDSRNVYNRWQGTDLSWYSQNGIIVFTVDHRGSGQFGRNGLDYLHRSLGRWEILDYA
                     DAVEWLRTKSFVDPARIGITGSSYGGYMTCMALTRGADYWTHGFAGSSVTDWRLYDNI
                     YTERFMDTPGDNPEGYRQGSALTWAENYRGKLYMTHGDMD"
     gene            2132..>3225
                     /locus_tag="PLZ75_12960"
     CDS             2132..>3225
                     /locus_tag="PLZ75_12960"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOS73347.1"
                     /translation="MTRAILPLSVLMLLIGCSTDRSRIERAVRSISVRSIERYVAELA
                     SDRFMGRKPFTEGEEITISYLADQLRKIKFGPAFDGSYFQKVPMAEITSHVDEILFSK
                     PSGTTLRFRTPDEAAVISPLESGHITAVDLPLVFAGFGINAPEYGWNDYSGLDVRGKM
                     VIVLVNDPGLYTGDSALFRGREMTYYGRWTYKYEEAARQGAAAVLIIHDTEGAGYEYN
                     IPRKSSISPRLYIQGHGQNNPLCDFTGWLPGDSADRLLKACSLSTDSLRAEACKQGFR
                     GFPLNINASVSCRNEIRYDESSNVAGILKGSRNPDECIVFTAHWDHFGIGESENGDSI
                     YNGAVDNATAMAWALAAGKAFSSLGKRPER"
BASE COUNT          802 a          796 c          743 g          884 t
ORIGIN      
        1 cgtccatgtc accgtgtgtc atgtagagct tccccctgta attttccgcc cacgtaagtg
       61 ctgagccctg tctgtagcct tccggattat ccccgggagt atccataaac ctttctgtat
      121 agatattgtc ataaagcctc cagtcggtta ctgatgatcc tgcaaagccg tgggtccagt
      181 aatccgcccc cctggtaagg gccatgcagg tcatgtatcc gccgtatgaa cttcccgtta
      241 ttccgatcct tgccggatcg acaaatgatt ttgtcctcag ccattccacc gcatcggcat
      301 aatccagtat ctcccaccgg ccaagtgaac ggtgaaggta atcaagcccg ttccggccga
      361 actgtcccga accccggtga tcaactgtga aaacaattat tccgttttgt gaataccacg
      421 acagatccgt gccctgccat ctgttataca catttctgga gtccggtccg ccatagattc
      481 tgaacaacac cggatatttt tccgcagggt caaaattaag cggataggtt attatggccg
      541 gcatattgaa caaaccgtca gatgtcggaa tcctgatcat ttctgaacgg gcatgccttc
      601 cggggtcaaa ggaaggtatt tcatacctgt gtatttcacg tatcatcctt ccctttctgt
      661 tgtaggctat tattgatccc gggtcagaaa ggctgttcca ggtatcaata tagtaagttc
      721 ccttgggcga gatgcttata ttatgactgc cttcacccgt tgtaagcctg acaagattct
      781 ttccgtcaag tccgactctc catgcatgaa ggtcggttga ctccggtccc gttgcagtga
      841 aatagacggt tttctgatct tcatcaacac gtattactga agtcaccctg aagtcaaaat
      901 cggtgagctg cgcgatcagt ctgccctccc atgcgtaaaa atacaggttc tcccagtcat
      961 tcataaaact tctgataatg aatcctgatc cgtttttcat aacgtaaata tcttccatga
     1021 attcaaccca ggtccttctc cattcattgt agatttccct gtagtcaccc gtgagcggat
     1081 cagcaagtat tatactgaga ttgttctgat cgcggttcag tatctgaact gccagatgcc
     1141 tgctgtcggg agtccagaac ggccaggcta tatactggtc tgctgaataa tcagttttta
     1201 cccacactgt ttctccggta gccaggtctg caattcccat tctcaccctg gggttgggat
     1261 ctcctgcctt cggataggga acctgctcga gggttccgtg aatgccatcc tcttcatcca
     1321 gtctgttaag tgtaaacatc ggaacatcac tttcgtcggt cctgagatat gctatcctcg
     1381 atccgtcggg agaccaccag aatgctgcga aacggcttgc cctgccaagt atttcttcca
     1441 tataaaccca tgaagcatat ccgttatata tacggtctga ggcgtcatga gtcagtcttg
     1501 tttccttaaa actcaccaga tcatacacat acagatccct gttgcgggta tatgcaatct
     1561 tgctgccgtc atcacaaaat ctcacattca cttccggggc attgtcatcc gtcaatctcc
     1621 ggaattcttt gtcacccgtt ctgaaataaa acagatcatt gtcaatgatt cttatcaggc
     1681 ctgaattgtc agggctgaca atgtcgtccg gtttcggctg tgttccttcc gggagggatt
     1741 tttcaagaag atcccgtgag gtttcaggaa gactgattac ggtgcctttg ccagtacgga
     1801 tgtcaacact ctgaattatt gcctttccct cagaatccca ggtcctgaaa aggtaatgac
     1861 tgtcatcaag ccatcctgta acagcagcct ggcccggatt ccggccgggc atctgtgctg
     1921 caagctgaga gacggaaaga aggaaaaaaa tctggataat aataaatact cctttcggaa
     1981 aattgctttt catgtttata tttttttgtt tcaaattatg gtttttccgt tgtaaaaata
     2041 aaaattaaga gctgtccttt attattttcc acacagtttt caaacataac atttttgcat
     2101 taatttcgct gcacaaatca acctgaccct gatgacccga gcaattctcc ccctgtcggt
     2161 cctgatgctg ctgattggct gttcaacgga cagatcccgg attgaaagag ctgtaaggag
     2221 catatcagtc aggagtattg aaaggtatgt tgccgagctt gcaagtgaca ggttcatggg
     2281 cagaaaacct tttactgaag gagaggaaat cactatatca tacctcgcgg atcagctgcg
     2341 gaaaataaaa ttcggtccgg catttgacgg gagttatttc cagaaagtgc ctatggccga
     2401 gatcacttcc catgttgatg agatactttt cagtaaacct tcaggcacaa ccctgcgttt
     2461 ccgcacaccc gacgaggctg ccgtgatcag tcccctggaa agcggtcata tcactgccgt
     2521 ggatcttccc cttgtgtttg caggctttgg aattaatgcc cccgaatatg gctggaatga
     2581 ttattccgga cttgatgtca ggggaaagat ggtgattgtt ctggtaaatg atccgggatt
     2641 gtatacaggc gactctgctc ttttcagggg aagggaaatg acttattatg gcagatggac
     2701 ctacaaatat gaagaggcag cccggcaggg cgcagcagct gtacttatca tccatgacac
     2761 tgaaggggct ggctatgaat acaatattcc ccgcaagagc tccatttcgc ccaggctgta
     2821 tattcagggc cacggtcaga acaaccccct ctgcgacttt acgggatggt tgccgggaga
     2881 ttccgcagac aggctgctga aagcctgcag tctcagcacc gacagtctga gagctgaagc
     2941 atgcaaacag ggattcaggg gttttccgct taacataaac gcatcagtct cctgccggaa
     3001 tgagataagg tatgatgaat cgtcgaatgt ggcaggaata ctgaaaggat cccgtaatcc
     3061 ggatgaatgt attgtgttta cggcccactg ggatcatttt ggtatcggcg agagcgaaaa
     3121 cggtgattca atatacaacg gtgcagttga caacgcaacg gcaatggcat gggctcttgc
     3181 tgccggtaaa gctttctcct ccctcggaaa gaggccggaa aggag
//
LOCUS       DAOSPX010000351         3201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_27211_length_3201_cov_4.321996, whole genome shotgun sequence.
ACCESSION   DAOSPX010000351 DAOSPX010000000
VERSION     DAOSPX010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27211_length_3201_cov_4.321996"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLZ75_12965"
     CDS             <1..507
                     /locus_tag="PLZ75_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009435054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOS73348.1"
                     /translation="ETPEIPGTPEKEKPLRVGDSVRMTETMAAGEITEIKDNMIRVES
                     GSFSFVVSAEKIERISRSELRKMLKRGPSYRESSPELMQRRLDFSPEIDIRGMRAEEA
                     ILRVQDLLDNALMVQHRNLRILHGKGNGILRQVIRQYLATVDIVGSFRDEHVDMGGAG
                     VTLVEMDF"
     gene            complement(545..1732)
                     /locus_tag="PLZ75_12970"
     CDS             complement(545..1732)
                     /locus_tag="PLZ75_12970"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS73349.1"
                     /translation="MKTSSFILRLVFLAALSLQLAGQGNQVPGVIVNHIPASAKTYIG
                     SPSVCILPNGDYVASHDHFGPGSTEHERALTAVFRSADRGLTWQKISEINGQFWSNLF
                     FYDNALYIMGTWKHHGNLIIRRSDDNGETWTEPLDSRTGLIREGEYHTAPVPVIIHRG
                     RIWRAVENAKSFTSAWGIRYSATVFSAPLGSDLLDASNWTETNGLVHDSAYLEGRFRG
                     WLEGNAVAAPGGKVLNILRVSTTEPGRDMAAIVNISDDGLNAAFDPANGFIDFTGGAR
                     KFSIRYDPKSKRYWTICNIVTKEHTDRNAGSVRNTLVVKSSPDLKHWTVHKVLLYHPD
                     VEKHGFQYVDWQFDGKDIIYLSRTAYDDEYGGANNYHDANYLTFHRINNYRKLIKKEI
                     SEL"
     gene            complement(1861..2127)
                     /locus_tag="PLZ75_12975"
     CDS             complement(1861..2127)
                     /locus_tag="PLZ75_12975"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOS73350.1"
                     /translation="MIHVEKATEEKLARLNVRSWPVWTKEVSEFPWKYDEAETCYILE
                     GDVEVIPAGGQAVRIGKGDFVVFSSGLECIWKVHSPVRKHYKFG"
     gene            complement(2239..>3201)
                     /locus_tag="PLZ75_12980"
     CDS             complement(2239..>3201)
                     /locus_tag="PLZ75_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73351.1"
                     /translation="PSMVLDLSNKYGDIDIQTSETGRVIIDVRVTLRYPNREKAERLF
                     SYIDVEFSEDPDRISARTVISSKFSYSGWSSDSRRFRIDYNVKMPEDMDLTLVNRYGN
                     TILDDLSGHAALDIKYGNLTAGKLLRENEKPLNSIILAYGNGSVEQAGWLDAVFRYCS
                     NFRISKSQALLIDSRYSKIQLGTVSSLVGDLKYDNLRIDKINNLVLNSGYTVTNIGTL
                     TRKLEFDVSYGSCSIERVPAEFESIDLKSRYTSVSIGIDDDANYRLNGTASYGGLKFR
                     ESKYRNVMRKAEGSSTTVDGIIGNEESPASRVKIDAAYGSVKLF"
BASE COUNT          803 a          747 c          743 g          908 t
ORIGIN      
        1 gaaactcctg aaattcccgg aactccggaa aaggaaaagc ccctccgggt gggtgacagt
       61 gtcaggatga cggaaacgat ggcagccggt gaaataacag aaataaaaga caatatgatc
      121 cgtgtggaaa gcggaagctt cagctttgtg gtatcggctg aaaaaatcga gagaataagt
      181 agatcggaac tgagaaagat gctgaaacgg ggaccgtcat acagggaaag cagtcctgaa
      241 ctgatgcagc gcagactgga tttcagtcct gagatagata tccggggaat gagggccgag
      301 gaggctattc tccgggtgca ggatctgctc gacaatgcgc ttatggtaca gcaccgcaac
      361 ctgagaatac ttcacgggaa gggaaacggc atactccggc aggtaatacg gcagtacctg
      421 gccactgtgg atatagtcgg atcattccgc gatgagcatg ttgacatggg aggggcggga
      481 gtcacccttg tcgaaatgga tttttagcct tcgcaggatt taaaaccatt cccggaatca
      541 aatcctataa ttcggagatt tcctttttaa tcagtttcct ataattattt atcctgtgga
      601 aggtaagata attggcatca tgatagttgt ttgcacctcc gtattcatca tcgtaggccg
      661 tacgtgacag atagattatg tcttttccgt caaactgcca gtcaacatac tggaatccat
      721 gtttttcgac atcgggatgg taaagaagca ctttatgaac ggtccagtgc ttcagatcgg
      781 gcgagctttt aacaacaaga gtatttctga cagatccagc attcctgtct gtgtgttcct
      841 ttgtcacaat attgcatatt gtccagtagc gtttgctttt cgggtcatac ctgattgaga
      901 atttccttgc acctcccgta aaatcaataa atccgtttgc cggatcaaat gcggcattca
      961 gtccgtcatc gctgatattc acaatggctg ccatgtcccg tccgggttca gtagttgaca
     1021 ccctcagtat gttcagcact ttccctccgg gagccgccac tgcattacct tcgagccagc
     1081 ctctgaaacg gccctcaaga taggccgaat catgaacaag gccgtttgtt tctgtccagt
     1141 ttgatgcatc cagaagatct gatccgaggg gtgccgagaa gactgtggcg ctgtaccgga
     1201 tcccccaggc actggtaaac gattttgcat tttcaaccgc cctccagata cggcctctgt
     1261 gaatgatcac aggcacgggt gccgtatgat attccccctc acgtatcagg cctgtccggc
     1321 tgtccagggg ttctgtccat gtttcaccgt tgtcatcgga gcgccgtatt atcagattgc
     1381 cgtgatgctt ccatgtcccc atgatgtaga gagcgttatc ataaaagaac agattggacc
     1441 agaactgtcc gtttatttcc gagatctttt gccatgtcag gcctctgtcg gctgagcgga
     1501 aaacagcagt gagagctctt tcgtgttcgg tgcttcccgg tccgaaatga tcatgggaag
     1561 ccacataatc cccgttagga agaatgcaga cagacggaga tccgatatat gttttcgctg
     1621 atgccggaat atgattcact attactccgg gtacctgatt tccctgtccg gcaagctgca
     1681 atgaaagggc ggcgagaaag accaggcgca aaatgaagct tgatgttttc atgtaaatgt
     1741 tcattaatga gtatcataaa tgcaggcaaa aacctgtgtg cagaccactg caggaatatt
     1801 tgtctggctg cttacgggca ggaaaataca gggaagcatt ccggatgaca gtattggtct
     1861 ttatccgaat ttataatgct tccttacggg agaatggact ttccatatgc attcaagtcc
     1921 actgctgaac actacaaaat cgcctttccc gattctgacg gcctgtccgc cagccggtat
     1981 tacttccaca tctccctcaa gtatgtagca tgtttcagcc tcatcatact tccagggaaa
     2041 ttcggacact tccttggtcc ataccggcca ggatctcaca ttaagtcgtg cgagtttttc
     2101 ctcggtcgct ttttcaacat gtatcattgc aattcttttt attttaatgc aaaggtaaga
     2161 atgaatgaaa aataaaaaag ctccgttgca gacggagctt ttattccggg gttatcccgg
     2221 cagttttcaa aaaagttttc aaaaaagttt gactgaaccg tatgccgcat ctatttttac
     2281 ccgggatgcg ggagattctt cgtttcctat tattccatca acagttgtgg agctgccttc
     2341 cgcctttctc attacattcc tgtatttgct ttccctgaat ttcagtccgc catatgaagc
     2401 agtcccgttc agtctgtagt ttgcatcatc gtctattccg atgcttacac tcgtatagcg
     2461 cgacttcagg tctattgatt caaattctgc aggaaccctt tcaatactgc aggatccgta
     2521 ggaaacgtca aattcaagct tcctggtgag tgttcctatg tttgtaacag tatatccgga
     2581 attcagtaca agattgttga ttttgtctat tctgagatta tcatatttta aatcgcccac
     2641 gagggagctt accgtgccaa gctggatttt tgagtatcgg ctgtctatca gcagagcctg
     2701 gctttttgaa atcctgaaat tgctgcaata cctgaatact gcatccagcc agccggcctg
     2761 ttctacagaa ccattgccgt atgccaggat gatgctgtta agcggtttct cattttcccg
     2821 caacagtttg cctgctgtaa gatttccgta ctttatatcc agggcggcat ggccggacag
     2881 atcgtcaagt atggtgtttc cgtatctgtt taccagtgtg aggtccatat cttcaggcat
     2941 ttttacgttg taatcaatgc ggaagcgtct tgaatcactg ctccaacctg aataactgaa
     3001 ttttgaactg ataactgtcc tggctgatat acggtccgga tcctcgctga attccacgtc
     3061 tatatagctg aaaagccttt cagccttttc cctgttaggg tatctgagag taactcttac
     3121 atcaataata acccggccgg tttcggatgt ctgtatatcg atatccccgt atttgttgct
     3181 gagatccaga accattgagg g
//
LOCUS       DAOSPX010000352         3200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_27227_length_3200_cov_4.465183, whole genome shotgun sequence.
ACCESSION   DAOSPX010000352 DAOSPX010000000
VERSION     DAOSPX010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27227_length_3200_cov_4.465183"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLZ75_12985"
     CDS             complement(<1..531)
                     /locus_tag="PLZ75_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOS73352.1"
                     /translation="MHRLFRLFINRRLLIATIFVIIAGIGIYSWIVLPVDAYPDIADV
                     TVEVITQVSGLATEEIEQQITIPLERELNGLPGLQTMRSKNLFGISNILLVFQDGIDD
                     YWARQRVQERISGIDLPFDATPEIGPLTSPTGEIFRYYIESNSKSLRELTDLQRWVII
                     PRLKQITGVADVQNFGG"
     gene            complement(544..1713)
                     /locus_tag="PLZ75_12990"
     CDS             complement(544..1713)
                     /locus_tag="PLZ75_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS73353.1"
                     /translation="MPRILLPILLLIIVSCNNQPVHEKTVPDHTRERTGQRRRREAKR
                     RQEEHRHEIFHLKNDSLRVRDPLLLANLKTDVVHPREYHYELTTFGIVKPVPGNYAEV
                     SVPFDGRIIRSNVKLGQKIAAGTPLFEIFSPDYLESVRTFLEAKREKEIREINYRRKK
                     DLFDSEIISRKEYEEARLEYDLADKEFEKNLELLKIYKQDTDNVDISRPFSVVSPIAG
                     EIVRNEIMVGQFLRTDAEPVITVASLDRVRIVANVKEKDLYAVGADDEVEIISESMPD
                     RPLRGRIIYISDIMNEQTRSVEVYVECGNPDKILKSGMFVTVNFRHTMKDVIIIPSEA
                     VYQDSEVCYLFLQTGEGAYARREVSVKSVGNNLLLVRSGLDKGNIIVTDGGIYLR"
     gene            complement(1799..2167)
                     /locus_tag="PLZ75_12995"
     CDS             complement(1799..2167)
                     /locus_tag="PLZ75_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73354.1"
                     /translation="MKKDRNIILRIQGSALLTIFLLLFAGNNFFHHAHQVDDRIIVHS
                     HPFKSDSQGNPVHKHPATDYLLIHLFNTIVFCFIFSITRAGTVSGPAYTIVPHYRNPF
                     FSLISFLPLKFRGPPPLRLL"
     gene            2517..>3200
                     /locus_tag="PLZ75_13000"
     CDS             2517..>3200
                     /locus_tag="PLZ75_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOS73355.1"
                     /translation="MVNSTLFFIIPVLSLLVFAVPKHLKHYFTLLFLLAGMVLTTSWS
                     TGILAGSAGIREIPLFSPSYGTGITLVIDNLAAFFILVINITVLTGLLYARGYLAPYH
                     QRMPSFRFSIHYFSYIWLWLSMLMVVMLREGVAFLIAWEIMALSSFFLVIFEAEERNI
                     LKTGISYLIQMHIGMFFILIAFLLVQKATGSMSFDALAGYFAVHRNLPVFLLFFTGFA
                     IKAGFIPLHT"
BASE COUNT          773 a          734 c          694 g          999 t
ORIGIN      
        1 gccgccaaaa ttctgaacgt cggccacgcc cgtaatctgt ttcagccggg gaatgataac
       61 ccatctctga agatcggtaa gttccctgag actcttactg ttgctttcaa tataataacg
      121 gaagatctca cctgtgggag atgtgagggg gcctatttca ggagttgcat caaaggggag
      181 gtcgataccg ctgattctct cctggaccct ctgccgtgcc cagtaatcat caataccatc
      241 ctgaaatacc aggagaatgt tagaaatgcc gaaaagattc ttgcttcgca ttgtctgaag
      301 gcccggaagg ccgttcagtt cgcgttcgag aggtattgtt atctgctgtt caatctcttc
      361 tgttgcaagg ccgcttacct gggtgataac ctcaaccgta acatcagcga tatccggata
      421 ggcgtctacg ggcagaacaa tccaggaata tattcctatg ccggctatta taacaaagat
      481 ggtggctatc aggagccttc tgtttatgaa taatctgaat aatctatgca tggcaacaca
      541 ctcttagcga aggtatatac ctccatcagt tacaataata tttcccttat caagccctga
      601 acggaccagc agcagattat tgccaactga tttcacactg acctctcgcc tggcataagc
      661 gccttctccg gtctgcagga agaggtaaca tacttcacta tcctgatata cagcttcaga
      721 aggaatgata atcacatcct tcattgtgtg ccggaaatta acggtgacaa acatacccga
      781 tttcagtatc ttgtccggat tgccgcattc aacatatact tcaaccgaac gtgtctgttc
      841 gttcatgata tcactgatat agataatcct gcccctgagc ggcctgtccg gcatgctttc
      901 acttattatt tccacctcgt catctgctcc tacggcataa agatcttttt cctttacatt
      961 ggccacgatt ctgaccctgt cgagactggc aacggtaatt accggttcag catctgtcct
     1021 gaggaactgc cccaccatta tttcgttcct gacgatctcg cctgctatgg gcgagacgac
     1081 gctgaaggga cgtgatatgt caacattgtc agtgtcctgt ttgtaaatct tcagcagttc
     1141 aagatttttc tcaaactcct tgtctgccag atcatattcc agcctggcct cctcatattc
     1201 ctttcttgaa atgatctccg aatcgaacag gtcctttttg cgtctgtaat ttatttccct
     1261 gatttccttt tcccgctttg cttcaagaaa agtcctgacc gattcaaggt aatcggggga
     1321 aaatatttca aacagcggag tgcctgctgc aattttttgg cccagtttga catttgatct
     1381 gattatcctg ccgtcaaaag gtacagatac ttcggcataa tttccgggaa ccggtttcac
     1441 aataccgaat gttgtcagtt cataatgata ttccctgggg tggactacat cggttttcag
     1501 attcgcgaga agcagggggt ctcttactct gagactgtca tttttcaggt ggaaaatttc
     1561 gtgcctgtgt tcttcctgcc gtctttttgc ttctcttctt ctccgctgcc ctgttctttc
     1621 ccgagtgtgg tcaggtactg tcttttcatg aacgggctgg ttattgcagg atacaatgat
     1681 cagtaataat attggtaata aaatccttgg cattttttta gacttaaaat atcaatattg
     1741 ggggggtgaa acagttttaa gacctgtagc ccttttcaga ttatccggct tcgggatatc
     1801 agaggagtct gagcggggga ggaccccgga atttcagggg cagaaaagaa ataaggctga
     1861 aaaagggatt tctgtagtgc gggacaatcg tgtaagcggg gcctgacaca gtaccggccc
     1921 gggttatgct gaagatgaag cagaaaacaa tagtgttgaa aagatgtatc aggagataat
     1981 cagttgcagg atgcttatgt acgggattgc cctgtgaatc tgatttaaag ggatgggaat
     2041 gaacaattat tctgtcatca acctgatggg catgatgaaa gaagttattg cctgcaaata
     2101 agagcaaaaa tatggtcagt aacgctgacc cctggatcct gagtatgata tttctgtcct
     2161 ttttcattga gcttggacgc aaatatacat aaataggata ataaactggt tcattgattg
     2221 accgttttta acctgatcct ctcatactgc tcagtgttta atccctcatc ctgctattaa
     2281 tcactccttg gtcataaccc cggggtttcg ctcttaaatc tttcgtaccg tgtgtcctaa
     2341 atattttaac aataaaagtt cagaactctg tcagtcatca gagatcctcg cccggctgga
     2401 ggggtgtgcc tgaaactttg ctgaacatca ggctaataca agcctgaaat ttagttttcc
     2461 ccgtatttgt ctttcagctg atattttata catttgtgtg caaatttctg gtcttaatgg
     2521 ttaattccac cttatttttc attatccctg tcctttcgct tctggttttt gcagtaccga
     2581 agcatttgaa gcattacttc acgctgttgt ttctgctggc cggcatggtt ctcaccacgt
     2641 catggagtac cggcattctt gccggatcag ccggaatcag ggaaataccg ctgttttctc
     2701 catcttacgg cacaggaatt actcttgtaa tcgataatct tgcagccttc ttcatcctgg
     2761 tcatcaacat tacggttctc acaggccttc tgtatgccag gggctatctt gctccatatc
     2821 atcagaggat gccgtccttc cgtttttcca ttcattattt ttcgtatatc tggctctggc
     2881 tgtcaatgct gatggttgta atgctgaggg aaggggtggc ttttcttata gcctgggaga
     2941 taatggctct ttcatccttt ttccttgtga tttttgaagc ggaggaaaga aatattttaa
     3001 agaccggcat cagttatctc atccagatgc atatcgggat gtttttcata ctgattgctt
     3061 ttttactggt tcaaaaggcc acgggcagta tgagttttga tgcccttgcg ggctattttg
     3121 ccgtacatcg caatctgcct gtttttctcc tgttttttac aggctttgcc atcaaggccg
     3181 gattcatccc cctgcatacc
//
LOCUS       DAOSPX010000353         3181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_27457_length_3181_cov_3.595329, whole genome shotgun sequence.
ACCESSION   DAOSPX010000353 DAOSPX010000000
VERSION     DAOSPX010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27457_length_3181_cov_3.595329"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PLZ75_13005"
     CDS             complement(<1..924)
                     /locus_tag="PLZ75_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOS73356.1"
                     /translation="METVKLTIDNKTIEVPKGTTIHKAARELGIEIPVLCYMRLDDLN
                     IENKPAGCRICVVEVEGRRNLAPSCATSCEEGMVVRTHTTRVLNARRTVMEFILSDHP
                     KDCLVCAKSGNCDLQSLAQYLGIREIPGEEIAEMSTYRKDTSPSIIRDLDKCILCRRC
                     EMMCNTVQTVGALSAVNRGFMATVAPAFEQNLEHSTCTYCGQCVAVCPTGALTEVDHT
                     AEVLRALADPAKTVIVQTAPAVRAALGELFGMKPGTLVTGKMVTALRALGFDYVFDTD
                     FAADLTIMEEGTELLDRLQRHLSGDKSARLPI"
     gene            complement(942..2735)
                     /locus_tag="PLZ75_13010"
     CDS             complement(942..2735)
                     /locus_tag="PLZ75_13010"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOS73357.1"
                     /translation="MAKERIQIMVCGGTGCTGDQGEALLDNLKLELEANALENSVNVV
                     KTGCMGLCSMGPNIKILPDNIIYTGVRPDDAREIVSEHIMKGRRIERLLFTDPGSGEK
                     LATEKELSPHRKQLRIALRNCGIIDPESIDDAIAHDAYEALATALTNMKPLDVIKLIK
                     DSGLRGRGGGGFPTGIKWEIAFNNKSDVKYVVCNADEGDPGAFMDRSILEGDPHAVLE
                     AMAICGYAIEAAKGLIYIRAEYPLAVSRLNTAIRQAREYGLLGTNIMGSDFSFDIEIK
                     YGAGAFVCGEETALIHSMEGDRGEPTVKPPFPAVSGFREKPTIVNNVETYACISPIIL
                     KGSEWFSSIGTEKSKGTKVFALAGKINNVGLIEVPMGTTLREVINEIGGGIRNGKKFK
                     AAQTGGPSGGCLTEKHLDTPIDFDNLIAAGSMMGSGGLIVMDEDDCMVSVAKFFLEFT
                     VEESCGKCSPCRIGNKRMHELLEHITKGKGVPADLERLKNMCHVIKDTSLCGLGQSSP
                     NPVLSTLENFREEYEAHVTVKKCPAGKCKDLMEYFIIAANCVGCTACARVCPVGAISG
                     ERKEPHVINPEICIKCGACMEKCKFDAIIIQ"
     gene            complement(2760..3128)
                     /locus_tag="PLZ75_13015"
     CDS             complement(2760..3128)
                     /locus_tag="PLZ75_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOS73358.1"
                     /translation="MPKINSPAELSKHKKEIREKLYGQEKILIRVHMGTSGLASGAKS
                     IYEFFREELEKRNIEATVINSGDMGYSFAEPTIEVRLPGREAVVFGNVDKIKADEIIE
                     KYIKNGELTDGILPANYRSI"
BASE COUNT          690 a          813 c          706 g          972 t
ORIGIN      
        1 tatgggcagt ctggcgctct tgtccccgct gaggtgcctt tgtagcctgt ccagtaattc
       61 cgtaccttct tccattattg taaggtcggc tgcaaagtct gtatcgaata cataatcgaa
      121 gccgagtgcc cggagagctg tgaccatttt tccggtaacc agtgtgccgg gtttcattcc
      181 gaaaagttct ccgagtgctg cgcggacggc aggtgcagtc tgaacgatga ctgtttttgc
      241 aggatcggcc agggcccgca gtacttctgc cgtatgatcc acttcggtaa gggctccggt
      301 ggggcagacg gcaacgcact ggccgcagta ggtacatgtg gaatgttcaa ggttctgctc
      361 aaatgcggga gcaacggttg ccatgaatcc cctgttcact gccgaaagtg cgccgactgt
      421 ctggacggta ttgcacatca tttcacagcg gcggcacagt atgcatttgt caagatccct
      481 gattattgag ggagatgtat ccttgcggta tgttgacatt tcagcgattt cttccccggg
      541 tatttccctt attccgaggt attgtgccag tgactggagg tcacagtttc ccgattttgc
      601 acatacaaga caatctttcg gatggtccga caggatgaat tccattacag tgcggcgtgc
      661 attgagaacc cttgttgtgt gggtcctgac aaccatgcct tcttcacagc tggtggcaca
      721 ggaaggagca agatttcttc tgccctcaac ctcaacgaca cagatcctgc atcctgcagg
      781 tttgttttcg atattgaggt catcgagcct catgtagcag aggacaggaa tttcaattcc
      841 aagctccctg gctgctttat gtatggtggt tcctttgggg acttcaatcg ttttattatc
      901 tattgtgagt tttactgttt ccatttccga caattctttt tttactgaat aatgattgcg
      961 tcaaatttgc atttctccat acatgcaccg catttaatgc atatttccgg attgatcaca
     1021 tgtggttcct ttcgctcccc gcttattgcg ccaaccgggc agacccttgc acatgcggta
     1081 cagccaacgc aattggcggc aataatgaag tattccatca gatccttgca tttcccggcc
     1141 ggacatttct taacggtaac atgagcctca tactcctccc ggaaattctc aagagttgag
     1201 agaaccggat tcggagaact ctggccaaga ccgcagagcg aagtatcctt tattacatga
     1261 cacatgtttt tcaggcgttc aagatcggca ggcactccct taccctttgt tatatgttcc
     1321 agaagttcat gcatcctctt gtttcctata cggcagggtg agcatttgcc acaggattcc
     1381 tccaccgtga attcaagaaa gaatttagca accgaaacca tgcagtcgtc ctcgtccatt
     1441 actataagtc cgcccgatcc catcattgat cccgcagcta tgagattgtc aaaatcgatc
     1501 ggagtatcca gatgtttttc agtaagacat ccgcccgaag ggcctcccgt ctgggcagcc
     1561 ttgaattttt ttccgtttct gatacccccg ccgatttcat ttatcacttc cctgagggtg
     1621 gttcccatcg gaacttcaat aagtccgacg ttgtttattt ttcccgcaag ggcgaaaacc
     1681 tttgttccct tggatttttc tgttccgatg ctgctgaacc attctgagcc cttgagtatt
     1741 atcggtgata tgcaggcata tgtttcaaca ttgttgacaa ttgtgggttt ttccctgaag
     1801 cctgatactg ccggaaacgg cggttttacg gttggttcac cgcggtctcc ttccatggaa
     1861 tggatgaggg cggtttcctc gccgcataca aatgcacccg caccatattt tatttcaatg
     1921 tcgaagctga aatcactgcc catgatattg gttcctagca ggccatattc ccttgcctgc
     1981 cggattgctg tattcagccg tgacacagcc agggggtatt ccgcacggat atagatcaga
     2041 cccttggcgg cttcgattgc atatccgcat attgccattg cttcaagtac ggcgtgcgga
     2101 tcaccttcca gtatggaccg gtccataaag gcaccggggt cgccttcatc agcattgcag
     2161 acaacatatt tgacatccga cttgttgttg aaagcaattt cccatttaat gccggtggga
     2221 aatccgcctc ccccgcggcc ccttaatccc gaatccttga tgagtttgat gacatccagg
     2281 ggcttcatgt ttgtcagagc cgtggcaagg gcttcatacg catcatgtgc tatagcatca
     2341 tcaatgcttt cgggatcaat aataccacag ttccttaatg ctatcctcag ctgtttgcga
     2401 tgcggcgaca gttccttttc ggtagccagt ttttctcctg aaccgggatc tgtgaacaga
     2461 agcctttcaa tccttcttcc cttcatgatg tgctcactga caatctcccg tgcatcatcc
     2521 ggcctcacac cggtgtaaat gatattgtca ggcagtattt tgatattcgg ccccatgctg
     2581 caaaggccca tgcagccggt tttcacaaca ttaacactgt tctcgagggc atttgcctcg
     2641 agttcaagct tcaagttgtc tagtaatgct tccccctgat cgcctgtgca tccggttcct
     2701 ccgcatacca taatctggat tctctctttt gccatggttt tcctccgtgg atggttttgt
     2761 taaatagaac ggtaattggc aggcagaatt ccgtctgtaa gctccccgtt cttgatatat
     2821 ttctctatta tctcatcagc tttgatcttg tctacattgc caaaaacaac tgcttccctg
     2881 cctggaagtc ttacctcaat cgtgggttcg gcaaaactgt atcccatgtc tcccgagttt
     2941 attacagttg cttcaatatt acgcttttca agctcttccc tgaaaaattc gtaaatgctt
     3001 tttgcaccgg atgccagtcc gcttgttccc atgtggaccc tgatcagtat tttttcctgt
     3061 ccgtataatt tttcacggat ttcttttttg tgcttactta attcggcagg agaattgatt
     3121 ttaggcatat ttgcttgatt tattgtttat tataacatcc tcatctatat cgtgtaatta
     3181 t
//
LOCUS       DAOSPX010000354         3173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_27530_length_3173_cov_3.835471, whole genome shotgun sequence.
ACCESSION   DAOSPX010000354 DAOSPX010000000
VERSION     DAOSPX010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27530_length_3173_cov_3.835471"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..797)
                     /locus_tag="PLZ75_13020"
     CDS             complement(15..797)
                     /locus_tag="PLZ75_13020"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOS73359.1"
                     /translation="MKKKILLITVVLLLFPFSGGAQVSAYKPGEKIRYTIHYGFITGG
                     EATLELNNDTLSGRQIWHSRLTGRTLGFVDLLFKVLDIYESYMDPATELPVRSIRNIS
                     EGNYKRYNVVDFDHKTRKDSAILTSDVSGVHVTQPGIHDIISCFYWFRNHVLPNIDTI
                     KKGDMITIMTWFTDELYPIRMRYIGTEEVKTRAGRINCYKFNPVTEKGRLFKTEEDVS
                     FWFSSDKNFLPVKIRFDIFVGAFIVDLDSYEGLKYPLEIKKK"
     gene            complement(834..1538)
                     /gene="tsaB"
                     /locus_tag="PLZ75_13025"
     CDS             complement(834..1538)
                     /gene="tsaB"
                     /locus_tag="PLZ75_13025"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOS73360.1"
                     /translation="MILCIETATDICSVALCRNNEVSFLRESEQGRSHASLLTVFIQE
                     LLTANNIDAGTLDAVAVSKGPGSYTGLRIGVSVAKGIAYGASIPLIGIETGLSMFHGF
                     RDYAKGKYTFVPGDLFCPLLDARRMEVYSIVFAWDGKIVRQTRAEIINNESFREFPAS
                     SRIFLFGDGAGKCRDVLKRDNVIIETGYRISASSLCRPASEAYSGHLFEDVAYFEPFY
                     LKDFLTSKPVKNILGK"
     gene            complement(1559..2812)
                     /locus_tag="PLZ75_13030"
     CDS             complement(1559..2812)
                     /locus_tag="PLZ75_13030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS73361.1"
                     /translation="MKNNKILKILIIVVAVLLIFAVIGKKAGWFGKAATVKVAVEFAE
                     RRMITETITANGKIQPEKEVKISPDVSGEIVELHVREGDQVTKGQLLIRIKPDIYISQ
                     RDRSVAAISSARARLAQAEAQFTQSELSFKRTRQLFEEETISRSEFEQAEASYNVAKA
                     EVDAANFSVISSEASLKEANENLVKTSIYAPMDGTVSMLLVELGERVAGTSMMAGTEM
                     LRIADLSRMEARVEVNENDIVRVNLGDTAIIEVDAYLDEKFTGLVTEIANSAKTTGVS
                     ADQVTNFDVKIFILPESYQKLASPGVNPLRPGMSATAEIRTETRENIITVPIQAVTTR
                     MDTSKVTARATRDEIRTLVFATDGKYAFARDVRTGIQDNSYIEIISGIEDSARIISAP
                     FSAISKKLSDSTLVEIVKREDLFSK"
     gene            complement(2836..>3173)
                     /locus_tag="PLZ75_13035"
     CDS             complement(2836..>3173)
                     /locus_tag="PLZ75_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS73362.1"
                     /translation="ISGSKLNIENSQYTLEAEKNQLYKTIQQSWADAVAALKKYSASM
                     KAVASMEESFRYTEQKFNVGMVTPVDYNAAKTQLLTAQSEMAQSKYEFIFKTKVLDFY
                     KGIPLNLNQ"
BASE COUNT          796 a          744 c          658 g          975 t
ORIGIN      
        1 ttattcttcc ttatttattt cttttttatt tccagagggt atttcagtcc ctcgtaacta
       61 tcaagatcaa caatgaatgc accgacaaat atatcaaatc gtattttaac cgggagaaaa
      121 tttttatcgg aagaaaacca gaaagacaca tcttcctcag ttttaaaaag cctgcctttt
      181 tcggtaacgg gattaaactt gtagcagttt atcctgccag ccctggtctt aacttcttca
      241 gttccgatgt atctcattct tatcgggtag agttcatcag taaaccacgt cataatggtt
      301 atcatgtcac ccttttttat ggtgtcaata ttgggaagga catgatttct gaaccagtaa
      361 aagcaggaaa tgatatcgtg gattcccggc tgtgtgacat gcacaccgct cacatcactg
      421 gtcagtattg ccgaatcctt tctggtttta tgatcaaagt caacaacatt gtatctcttg
      481 taattgcctt cgctgatgtt tcttatggat ctcaccggca gttccgtagc gggatccata
      541 tagctttcat aaatatcgag taccttaaag agaagatcca caaatccgag cgttcttccc
      601 gtcagccgtg aatgccatat ctgcctgccc gacaatgtat cattgttaag ctcgagcgtt
      661 gcctcgccgc cggttataaa gccgtaatga atggtatacc gtatcttctc accgggcttg
      721 taggcagata cctgggcccc gcctgaaaaa gggaacaaaa gcagtacaac tgttatcaga
      781 agtatttttt ttttcattag ctgtttcaga ggcaatggca attttcagac caattatttc
      841 cccaggatat tctttacagg tttcgaagtt aaaaaatctt tcagataaaa gggttcaaaa
      901 tatgccacat cttcaaaaag atgacctgaa tatgcctctg aagcaggccg gcacaatgat
      961 gatgcggata tcctgtaacc ggtttctata atcacattat cccttttcag aacatcccgg
     1021 catttgcctg caccgtcccc gaaaagaaaa atacgggatg aggccggaaa ctctctgaat
     1081 gattcattat taattatttc tgccctggtt tgcctgacta ttttgccatc ccaggcaaag
     1141 acaatggaat atacttccat tcttctggca tcaagcagag ggcaaaatag atcccccggc
     1201 acgaaagtgt atttcccttt cgcgtaatcc ctgaatccgt gaaacatgga aagacctgtt
     1261 tcaataccga tgagaggtat tgaggctccg tatgcaatgc ctttcgccac agaaaccccg
     1321 attctgagcc cggtatagga tcccggcccc ttgctgacgg caaccgcgtc aagagtgcct
     1381 gcatcaatat tgtttgcggt aagtaattcc tgaatgaaaa ccgtaagcag cgaggcgtgg
     1441 gaacgcccct gttcactctc cctgagaaag cttacttcat tgttccggca gagtgcaacg
     1501 gaacaaatat cggtggctgt ttcaatacac agaatcatcg taaaaatccc ggaagtgcct
     1561 atttgctgaa aagatcctct ctttttacta tttccacaag agtgctgtcg gacagtttct
     1621 tgcttatggc actgaaggga gcggaaatta ttcttgcact gtcttcaata ccggaaataa
     1681 tttctatata tgaattatcc tgaatgcccg ttctgacatc cctggcaaag gcatattttc
     1741 cgtcagtggc gaagacaagt gtgcgtattt catcccttgt tgcccttgca gtaacttttg
     1801 aagtatccat ccgggttgtg acagcctgta tgggcactgt gattatgttt tccctggttt
     1861 ctgtgcggat ctctgccgtt gcggacattc cgggcctgag gggattgact cccggtgaag
     1921 ccagtttctg gtaggattcc ggcagaatga aaatcttaac atcaaaattt gtcacctggt
     1981 cagctgatac gccggtagtt ttggcagaat tggctatttc ggtcaccaaa cctgtgaatt
     2041 tctcatcgag gtaggcgtca acttcaataa ttgcagtatc tccaaggttt actctcacta
     2101 tatcattctc attgacttcc acccgggctt ccattcttga cagatcagct atcctgagca
     2161 tttccgttcc ggccatcatg cttgttccgg caactctttc tcccagttcc acaagaagca
     2221 ttgagacggt gccgtccatc ggggcataga tcgatgtctt tacaaggttt tcatttgctt
     2281 ccttcagtga tgcttccgaa cttattacag agaaatttgc agcgtcaacc tcagccttgg
     2341 ccacgttgta tgaagcttca gcctgttcaa attccgatct ggaaattgtt tcctcctcga
     2401 aaagctgtct ggtcctttta aaggataatt cgctctgtgt aaactgcgct tctgcctggg
     2461 ccagcctggc acgggcggaa ctgatggcag ctacggaacg gtctctctgt gaaatataga
     2521 tatccggttt gatcctgata agcagctggc cctttgttac ctgatctcct tccctgacat
     2581 gcagttcgac aatttccccg gaaacatcag gtgatatctt gacttccttc tcaggctgga
     2641 ttttgccgtt tgctgttatg gtttcggtta tcatccgtct ttcggcaaat tcaactgcaa
     2701 cttttactgt ggcagccttc ccgaaccatc cggccttttt gcctataaca gcaaaaatca
     2761 gaagcacagc cacaactatt atcaggatct ttaatatttt attgttcttc attttgagat
     2821 tgatgatgaa ttggactact ggttaagatt aagaggtata cccttataga aatcaagaac
     2881 ttttgtcttg aaaataaact catattttga ctgtgccatt tccgactggg ctgtcagcaa
     2941 ctgtgttttt gcagcattgt aatcaaccgg ggtaaccatt ccgacattga acttctgttc
     3001 ggtatatctg aaggattctt ccatggaagc cactgccttc atgcttgcgc tgtatttctt
     3061 aagggctgcc acggcgtctg cccaggattg ctgaatggtt ttgtatagtt ggttcttttc
     3121 cgcctcaagt gtgtactgac tgttctcaat attcagtttt gaacctgata tgt
//
LOCUS       DAOSPX010000355         3168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_27593_length_3168_cov_4.542242, whole genome shotgun sequence.
ACCESSION   DAOSPX010000355 DAOSPX010000000
VERSION     DAOSPX010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27593_length_3168_cov_4.542242"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLZ75_13040"
     CDS             complement(<1..897)
                     /locus_tag="PLZ75_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS73363.1"
                     /translation="MAEDNKIIFTMHRVSKTYPPHRQVLKDISLSFFLGAKIGIIGLN
                     GSGKSTLLKIIAGVEKEFQGQLTFLPGYTVGHLPQDPLLEEERTVKEIVNEGVREIME
                     LMEEFETINNSFGDPEVYENAERMQVLMDRQAELQEKIDYHDGWNIEYRIGRAMDALR
                     CPDGDTLVSVLSGGERRRVALCRLLLSQPDVLLLDEPTNHLDAESVRWLETTLKQYKG
                     TVICITHDRYFLDNVAEWILELDRGEGIPWKGNYSSWLEQKARRLEIEEKGNIRRRKV
                     LERELEWVRMSPKARHAKSKARL"
     gene            complement(991..1368)
                     /locus_tag="PLZ75_13045"
     CDS             complement(991..1368)
                     /locus_tag="PLZ75_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73364.1"
                     /translation="MEENKMKKHVVVVGAIHIGFGFIGLIGALAIFFALRFARGFVTD
                     AEVAEMVLGFLSVSLPLLVGFLSTLALAGGIGLISFQPWARYLIIVVAALGCLNIPIG
                     TLKGVYSLWVLLQDETIRLFAGE"
     gene            1538..1771
                     /locus_tag="PLZ75_13050"
     CDS             1538..1771
                     /locus_tag="PLZ75_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73365.1"
                     /translation="MKRKILFTLLFALITWAVTSCEDLIRCKKCRLVSTDLLTGEVTY
                     DPNETEYCGAALAAIQATPPKTMGNVTTEYVCR"
     gene            2001..2420
                     /locus_tag="PLZ75_13055"
     CDS             2001..2420
                     /locus_tag="PLZ75_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS73366.1"
                     /translation="MKVNSLNLGDKKIVIVEDDLPSVRYYETLLRNSGASIKVFRNGK
                     EFVDYISTGNDRIDLVFMDFLVPLVNGIECTRIFRRREKKVPVIMVTGYASEQSRKEA
                     FLAGCNEYILKPILPEKIFSLLEKYLFESVSSHYLAG"
     gene            complement(2405..>3168)
                     /locus_tag="PLZ75_13060"
     CDS             complement(2405..>3168)
                     /locus_tag="PLZ75_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOS73367.1"
                     /translation="LKGNSIMKSQLIVLDILAHNNWERPVYYVTGFHNDTFGLEEYFQ
                     LEGLAYRLVPIRTENRGWLDYGRIKPDILYDNMMNKFAWGGANDVKVNIDYHHRRTLT
                     VVRARLNYARLAGRLAAEGRKEDALKVLDRCMEVLPVFNIPYDPYFPDLIDSYFKAGN
                     ADRAISLSTDFCNHYYETLDYYLKQKQYIISSAMYDIQLALQQTSKVADICEAAGQED
                     LANEINDRVTAYYVTYMGMQAPPPDADPGEIQPAR"
BASE COUNT          843 a          781 c          655 g          889 t
ORIGIN      
        1 aagtctggct ttggattttg catgacgggc tttcggagac attcttaccc attccagttc
       61 cctctcaagg acttttcttc tccggatatt ccccttttct tcaatttcaa ggcgcctggc
      121 cttctgttca agccatgatg aataatttcc tttccaggga attccctcac ccctgtcaag
      181 ttcaagtatc cattccgcaa cattatccag aaaatacctg tcgtgcgtga tacagataac
      241 cgttccctta tattgtttca gagttgtttc cagccagcgg accgattccg cgtccagatg
      301 gttggtcggc tcatcaagga gaagaacgtc cggctggctg agaaggagac ggcataaggc
      361 aactcttctg cgctcacctc cggaaaggac actgacaagg gtatcaccat caggacatct
      421 gagggcatcc attgctcttc ctatcctgta ttcaatattc catccgtcgt gatagtcgat
      481 tttttcctgc agttcagcct gccggtccat cagcacctgc atcctttccg cgttttcata
      541 gacctccgga tcgccgaagc tgttgttaat ggtttcaaat tcctccatca gctccattat
      601 ttcccttacc ccttcgttaa caatctcctt tactgttctt tcttcttcaa gcaaaggatc
      661 ctgaggcaga tggccaaccg tataacctgg aagaaaagta agctgtccct gaaactcttt
      721 ctccactccg gcaataattt tcagaagggt tgattttcct gagccgttaa gacctattat
      781 acctattttc gctcccagga aaaaggaaag ggaaatatct ttcaatacct gtctgtgagg
      841 cggataagtc ttacttaccc ggtgcatggt aaaaattatc ttattatctt cagccatatg
      901 tgtcctgatt tcccgctaat ataaaacata tctgtcagaa cagccagtga cgctgaatcc
      961 gggattgttg ccgtgagtcc ggaaaataat ttattccccg gcgaaaagtc tgatggtttc
     1021 atcctgcagc aggacccaga gagaatatac tcctttcagg gtaccgatgg gtatgttgag
     1081 gcagccaaga gcagcaacca caattatcag ataacgggcc cacggctgga aggatatcag
     1141 gccgattccc ccggctaatg ccagtgttga caaaaacccc acaaggaggg gcagactgac
     1201 tgataaaaac cccagaacca tttcagccac ttccgcatcc gttacaaatc ccctggcaaa
     1261 acggagtgcg aaaaaaatag ccaaagcccc gataaggccg ataaaaccga atccaatgtg
     1321 gattgcccct accacaacca catgcttttt cattttattt tcttccatga tattcaagag
     1381 ttattccgtt gatacaattt tcattcctgg ttaatgactg ccaatttaat gatttgatgc
     1441 atgctttccg ataaaacaat acatttacgg actttttaag tcatgccgca attatttaaa
     1501 aggatatttt ataattttga aacaaaacag gattcttatg aaaaggaaaa ttctgtttac
     1561 cctgttgttt gcactcataa catgggctgt cacttcctgt gaagatctga tccggtgtaa
     1621 aaaatgcagg ctggtttcga ctgatcttct tacaggagag gtgacttatg atccgaacga
     1681 aacggaatat tgtggtgctg cactggcagc catccaggct actccgccca agactatggg
     1741 aaacgtcaca acagagtatg tgtgccgtta aaatggcatg tcctgcgacg gacacatcac
     1801 atcaaaataa ttccggttcc aacagccgtt ttccactgaa aaccaatctc ctctccgcag
     1861 gccgcaaagc ctgaaaaaat ggactgcaca actgctgttt acatcatgtt gaaaagttgt
     1921 ttaaaaatgt aagccggtcc ggggttctcc tgtgattctt atcgtaaatt tacatttgca
     1981 atacttagtc tgattcatag ttgaaagtca atagtcttaa tctcggggac aagaagattg
     2041 tcatcgtgga agacgatctt ccgtcggtaa ggtattatga aacccttctc aggaattcgg
     2101 gtgcctccat aaaggttttc cgcaacggca aggaatttgt tgattatatc agcaccggaa
     2161 atgacaggat tgatctggtg ttcatggatt ttctcgtacc cctggtgaac gggatcgaat
     2221 gcacacgcat attccgccgg agggagaaaa aggtacccgt aatcatggtt accggctatg
     2281 catccgaaca aagcaggaag gaagcctttc tggcaggatg taacgaatac atactgaagc
     2341 ctatactccc ggaaaaaatt ttctcccttt tggagaaata tcttttcgaa agcgtttcaa
     2401 gccattacct tgccggctga atctctccgg ggtcagcatc cggcggtggc gcctgcatcc
     2461 ccatataagt tacataataa gccgtaaccc tgtcgtttat ctcattggcc agatcttcct
     2521 gtccggcagc ttcacatata tcagccactt ttgaggtttg ctgcagggcc agctgtatat
     2581 catacatggc agaactgatt atgtactgtt tctgcttcag ataataatca agcgtttcat
     2641 aataatgatt gcagaaatct gttgacaggc ttattgccct gtcagcgttt cctgccttga
     2701 aatagctgtc aatgagatcc gggaaatagg gatcataggg tatattaaaa accggaagca
     2761 cctccataca gcggtccaga accttcaggg catcttcctt cctgccttcc gcggccagcc
     2821 tgccggccag ccttgcatag ttcagccttg cccttacaac tgtcagggta cgcctgtgat
     2881 gatagtcaat attcactttt acatcattgg cgcctcccca tgcgaattta ttcatcatat
     2941 tatcatacag gatatccggt ttaatccttc cgtaatcaag ccagcccctg ttttcggttc
     3001 ttatcggaac cagccggtaa gccaggccct ccagctgaaa atattcttca agaccaaagg
     3061 tatcgttatg gaatccggta acataataaa cgggacgttc ccagttattg tgcgccagaa
     3121 tgtcaaggac aatcagctgg cttttcatta tcgaattgcc tttcagcc
//
LOCUS       DAOSPX010000356         3158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_27729_length_3158_cov_3.398969, whole genome shotgun sequence.
ACCESSION   DAOSPX010000356 DAOSPX010000000
VERSION     DAOSPX010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27729_length_3158_cov_3.398969"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1297)
                     /locus_tag="PLZ75_13065"
     CDS             complement(203..1297)
                     /locus_tag="PLZ75_13065"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOS73368.1"
                     /translation="MEKNLLPPDEDPPADPEISVSTVLTGYEIIWGMDFLPGGDLVFG
                     EKRGKLYRLSNGTVKEITGFPEVFPSGQGGLLDIRVHPDYAANGWIYASYSGSLPGGS
                     GQLNLIRFKIINDQVQNIENLFSSGGINTWYGHYGSRILFDRNKYLYLSIGEGGPTSL
                     GGPKSPNLNAQDTKSNWGKIHRFRDDGSIPDDNPVLPGNTGPSSIWSYGHRNPQGLAL
                     HPETGEVWSSEHGPMGGDELNIIKKGLNYGWPLYSIGINYDGSRISSGHTAEGIEPPL
                     YTWTPSIGVCGITFITSDSFGAWKGNLLASGLALQKLYRCVVKDNKVTEETELLTGYG
                     RVRNVIQGPDGNIYVSVEGPGRILRISPQP"
     gene            complement(1538..1747)
                     /locus_tag="PLZ75_13070"
     CDS             complement(1538..1747)
                     /locus_tag="PLZ75_13070"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOS73369.1"
                     /translation="MEREFEIIGEAMKRLEKIDSTLEISSKKQIINMRNRVIHGYDKV
                     DNEIVWGTIIRHLPKLKQEIDNLLK"
     gene            complement(1859..2140)
                     /locus_tag="PLZ75_13075"
     CDS             complement(1859..2140)
                     /locus_tag="PLZ75_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOS73370.1"
                     /translation="MDKIKALCIKHNVKSLHAFGSVCTDKFNTNSDIDLLISFKPMNY
                     AEYAETYFDLAEKFEILFKRPVDLITDKSLSNPYFIESINLTKIPVYEA"
     gene            2606..>3158
                     /locus_tag="PLZ75_13080"
     CDS             2606..>3158
                     /locus_tag="PLZ75_13080"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOS73371.1"
                     /translation="MSRLENSVTRNDLFRPGKCLVDIFIGSYSEAPSVIILDCTDTNN
                     DTCGQQGLALFNKYYNDYCYMPLHIYEGLSGKQITTILKPDRRDKQANIARLLQKLVL
                     YLRAQWPKTKIIVRGDSHFASKDFMDWTGGENNVGFITGLTGNEKLYELARVNIESAQ
                     REYNQYRKPVKRYHSFMYKAQSWE"
BASE COUNT          836 a          680 c          691 g          951 t
ORIGIN      
        1 gcgcgggaag ggatataatt gctttaaccg gggctgtatg aggaaaggga aatcccgccg
       61 gggctgtatg aggaagggga attacgccgg ggctgtgtga cccgggatta catcagaccg
      121 gataagcccg tttctctctg taattttttc atcaggcgtg agcccgggtg gtgcggaagg
      181 tgactttcca gaacgttttc cctcagggtt gtggtgagat cctgagtatc cttcccgggc
      241 cttcaacaga aacataaata ttcccgtccg gaccctggat cacatttcgt actctgccgt
      301 atccggtcag cagttcggtc tcttccgtga ccttgttgtc cttcaccacg catctgtaaa
      361 gtttctgcaa agcgaggccg gatgccagaa ggtttccttt ccatgccccg aaactgtcgc
      421 tggtaatgaa ggttattccg cagacaccga ttgaaggtgt ccaggtatag agagggggtt
      481 ctatgccttc ggcagtatgt cctgatgata tacgtgatcc gtcatagttg ataccgatgg
      541 aatacagcgg ccatccgtag ttgagtccct ttttgatgat gttcagttca tctccgccca
      601 tagggccgtg ttccgatgac cagacctctc ctgtttccgg gtgcagggcc agtccctggg
      661 gattccggtg gccatacgac cagatggagg aaggacctgt gtttccggga aggacgggat
      721 tgtcatcagg aatgcttccg tcatccctga accggtgtat ctttccccag tttgactttg
      781 tatcctgcgc attgagattg gggcttttcg ggcctcccaa agaagtgggt ccgccttcac
      841 ctatactcag gtagaggtat ttattcctgt caaacagtat gcggcttccg taatgcccgt
      901 accaggtgtt tattcctccg gaagaaaaaa gattttcaat attttgtacc tggtcgttga
      961 ttattttaaa gcggatcagg ttcagctggc ctgagcctcc gggcaatgaa cctgaatagc
     1021 tggcatagat ccagccgttt gcagcatagt ccggatgtac cctgatatcc agaagtcccc
     1081 cctgtcctga cgggaagacc tcgggaaagc ccgtgatctc cttcactgtt ccgttactga
     1141 gtctgtaaag cttacctctt ttttcgccga agaccaggtc gcctcccggc agaaaatcca
     1201 tcccccagat tatttcatag cctgtaagga ctgtgctgac acttatttcc ggatccgccg
     1261 ggggatcttc gtccggcggc agcagattct tttccatgca tgatgcgttt agcatcatga
     1321 tcaaccctga taataacaac cctgttttct gcaaaagcat attccgtatc aataaattac
     1381 ctcataatat aaacaaccgg cagcccggaa agtttaagat atctgcttta tttcgggtga
     1441 tgtggtgtta aagataaata cggggaaacc aaacactgct gtcatattct ctgagtgctt
     1501 tggtaaattt ttttcctgat cgagaaagag atattttcta tttcaacagg ttatcgattt
     1561 cctgctttag tttaggaagg tgcctgatta ttgttcccca aacaatttca ttgtcaactt
     1621 tatcatatcc atgaattacc ctattcctca tattgattat ctgcttctta cttgagattt
     1681 caagggtaga atcaattttc tccaatctct tcattgcttc accaattatt tcaaattccc
     1741 gctccacagc cctcgaagca ttttatctga catataagaa atgaagtctt ttttttcacc
     1801 aatgaatgtt tcaatcgatt ccacagattc tctgatgtcg aatagcagtt tttttatttc
     1861 aagcttcata aacaggtatc tttgtcagat ttatcgattc gataaaataa ggattactta
     1921 gtgatttgtc cgttattaaa tcgacaggcc gtttaaataa gatttcaaat ttttcagcca
     1981 aatcaaaata tgtttcagca tactcggcat aattcatggg tttgaaagat attaacagat
     2041 caatgtcgct gtttgtgtta aatttatccg tacaaacaga accaaatgca tgtaacgatt
     2101 ttacattgtg tttgatgcat agtgctttta ttttatccat attatctgtc aggattctat
     2161 gcattaaagt aatatttttg tttttcacaa agatagactt tcgaattgca tcctgcaaat
     2221 attctcttcc ccaactgatt acaagagcag gtttcctgat aaatcataag ggtgattata
     2281 taagatcgcc ttaagcgagt gaaaattatt ccttaagaag tagactatgg tactatctac
     2341 ctgcattaat atatttcaca tgtgttgata ttgcactttg ataccctctt tttctattga
     2401 atcataaagt gaaggtagct tgctaagccg gcctatggct gataataaga ggctgcatgt
     2461 ggacgataaa gaatttgccc atgactgtcc tgaagaatac cggattttaa gtgcggaagc
     2521 gatttcatcc agaacgtttt tactttgagg gggtgaatga attcccctct ctatcttact
     2581 ccagttgctt gcatcacagc caacgatgag ccgtttggag aactctgtaa caaggaatga
     2641 tctttttcga ccgggtaaat gtctggtaga tatttttata ggttcatatt cagaggctcc
     2701 ctctgtaatc attctagatt gtacggacac caacaatgac acctgtgggc aacaggggct
     2761 ggcactgttc aataaatact acaacgatta ctgctatatg ccgctgcata tttatgaagg
     2821 gctgtcgggt aaacagataa ccaccatttt aaaaccagat cgccgggata aacaggccaa
     2881 cattgccaga ttgttgcaga aactggtatt atatcttcgg gcgcagtggc caaaaacaaa
     2941 gatcatagta cgtggcgaca gccattttgc atcaaaagat tttatggatt ggaccggagg
     3001 ggagaataat gtcggattta taaccggatt aacgggtaat gaaaagcttt atgaactggc
     3061 acgggttaac atcgaaagtg cacaaagaga gtacaatcaa tatcgaaagc ctgtaaagag
     3121 gtatcattca tttatgtaca aagcgcagag ctgggaaa
//
LOCUS       DAOSPX010000357         3157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_27736_length_3157_cov_4.449065, whole genome shotgun sequence.
ACCESSION   DAOSPX010000357 DAOSPX010000000
VERSION     DAOSPX010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27736_length_3157_cov_4.449065"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLZ75_13085"
     CDS             complement(<1..588)
                     /locus_tag="PLZ75_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOS73372.1"
                     /translation="MKKNRRSFIQLAGAAGLSLAGGKYFNLHEDMTNKKTGMRDNLLH
                     KAYDSGLSGTEETAVNLIGPYGPWAAGLLEKDLPSLSFRRNEFTNSESWKKEAKNRLA
                     ERLGFPDIGLAPPVRKTREYSYDGLHIEELEWQLPYGRPTSALLLKPAGASGKLPGII
                     AFHDHGGNKYFGTRKITRTSEKQHPLIRAHQEQYYE"
     gene            857..3034
                     /locus_tag="PLZ75_13090"
     CDS             857..3034
                     /locus_tag="PLZ75_13090"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOS73373.1"
                     /translation="MTFLRHIFTAIVILGLSCYLSAQTRGVNRDKYRIHAMRTDEHIT
                     IDGILDENAWKLAEPTGKFQRVTPTDTGYAIAQTVAYVTYDNTNLYVGAICYDPTPGK
                     RPAESMKRDFSFNLNDNFMFFIDTYNDQTNGFAFGITAAGSQADGLQYEGIKVDYNWD
                     IKWRSAVKSYDDRWVVECAIPFRSLRYFGGEKEWGINFGRLDLKNNEKSAWAPMPRQF
                     PHASLPYTGTLVWEDPPEKAGPRFSLIPYGTAKVTSERENGQAAKWDWNAGIDGKMIL
                     STSMNLDLTLNPDYSQVEVDRQQIDLDRFELFYPEKRQFFLENSDLFSSLGRTGIQPF
                     FSRRVGLNVPVNAGLRLTGRFGDRWRIGLMDIQTGARDTIPSSNYAVTILQRNVFSRS
                     SIIAFLVNKQVTGSYSDNISGIMKYNRVTGLEYNMANPDNRWTGKVFYHQSLYPGAKA
                     DASAAGANLTYSSQFLKASLDQAWVGADFNAETGYIRRTGFYEVFPSVSYLFFGSQES
                     KILSHGPAVKFKTIFEPSSGMTDRQTEISYVIGWKSRDQLTLSVTEDFVRLSQAFDPT
                     NTFGVRLPAGSEYSWKNAGIKFDSDTRNLLYFSAEGLYGGYYNGNRLFLSGSLNYRVE
                     PYGSIAITANYNDIKLPEPYNSAELILIGPRLDITFTNNLFLTTFIQYNNQIDNLNTN
                     IRFQWRFAPVSDLFIVYTGNAFADDPMMNKNRGLVVKLSYWFN"
BASE COUNT          858 a          683 c          750 g          866 t
ORIGIN      
        1 ttcatagtat tgttcctgat gtgccctgat aagcggatgc tgtttttccg aagtcctggt
       61 aattttcctg gtcccgaaat atttattgcc tccgtgatca tgaaaggcga ttatccccgg
      121 aagctttcct gatgcacctg cgggtttcag caaaagagca cttgtcggtc ttccgtacgg
      181 aagctgccat tcaagttctt caatatgaag gccgtcatag ctatattccc tggtttttct
      241 aaccggtggc gctaatccta tatccgggaa tcccagcctt tccgcaagtc tgttttttgc
      301 ttccttcttc catgattctg agtttgtaaa ctcattccgg cggaatgaaa gggaaggaag
      361 gtctttttcc agaagcccgg cagcccatgg tccataagga cctataagat ttacagctgt
      421 ttcttccgtg ccgcttaatc cggaatcata tgccttatgc agcagattgt cccgcatacc
      481 tgttttttta ttagtcatat cttcatgtag gttaaaatat ttaccgccgg caaggcttag
      541 tccggcagct cctgcaagtt ggataaagct tcttcggttt tttttcatat cggcagattt
      601 tctgaaacac ttcggtgtaa aatacagatt attgtaaatg tacacaatta atcatttcac
      661 aaaacggcct ccttcacggt ttccggtcct gccggcccgc tgtcgtgcag acgtgttccc
      721 gctgtcgtgc ggacgtgttc ccgcagccgt tagggttatg aaactgccgg catcagttat
      781 gaaattacgg aaagaatatt gttgttatat ttagcacggg aaaaataccg ttttaaaatc
      841 tcaacaatat ttattaatga cttttttgcg acatatattc acagctatag taattctggg
      901 gctgagctgt tacctgagtg cacagaccag gggtgtgaac agggataaat accggattca
      961 tgcaatgagg actgatgagc atataacaat tgacggtata ctggatgaga atgcctggaa
     1021 gctggctgag cctacgggaa aattccagcg ggtgactccg acggatacgg gctatgccat
     1081 tgcccagact gttgcatatg tgacatatga caatacaaat ctttatgtcg gagccatatg
     1141 ttatgacccg acgccgggta aacggccggc ggaatccatg aagagggatt tttcattcaa
     1201 cctgaatgac aatttcatgt ttttcattga cacttacaat gatcagacca acgggtttgc
     1261 tttcgggatt acagcagccg gatcccaggc agacggttta cagtatgagg ggataaaggt
     1321 tgattacaac tgggatatca aatggcggtc agcagtaaag agctatgatg acaggtgggt
     1381 tgtggaatgt gcaattcctt tcagaagtct cagatatttc ggaggtgaga aggaatgggg
     1441 aattaatttc ggccggcttg atctgaagaa taatgaaaaa tcagcatggg ctcccatgcc
     1501 ccggcaattc cctcatgcct cacttcccta taccggaacc ctggtatggg aggatccgcc
     1561 ggaaaaagca ggaccgcgtt tttcactgat tccctatggt acagccaagg ttacttccga
     1621 gagagaaaac ggccaggctg cgaaatggga ctggaatgcc ggaatcgacg gtaaaatgat
     1681 actttcaaca tccatgaacc tcgatcttac attaaatcct gattattcgc aggttgaagt
     1741 tgacaggcag cagatcgatc ttgacagatt tgaattgttc tatccggaaa aaagacaatt
     1801 ctttcttgaa aacagtgatt tgttctcaag tctgggcagg acaggtattc agccgttctt
     1861 ttcaaggcgt gtggggctta atgtgcccgt caacgcgggt ttaagactta caggaagatt
     1921 tggcgacaga tggcggatag ggttaatgga catacagaca ggagccaggg ataccattcc
     1981 ctcaagcaat tatgcggtta ccatactgca acggaatgtt ttcagcaggt caagtattat
     2041 tgcctttctg gtcaacaaac aggtgaccgg gagttacagc gacaacattt caggaataat
     2101 gaaatacaac cgggtgaccg gactggagta caatatggca aatcctgata accgctggac
     2161 gggaaaagtg ttctatcacc agtcgctcta ccccggggca aaggctgatg catcggctgc
     2221 cggggccaat ctgacatatt cctcacagtt tctgaaggca agcctggacc aggcatgggt
     2281 gggggctgat ttcaacgcgg aaaccggtta catcagacga acaggatttt atgaagtctt
     2341 tccttctgtg tcctatcttt tcttcggatc gcaggagagt aaaatactga gtcacggacc
     2401 ggctgtgaaa ttcaaaacca tattcgagcc ttcctcgggc atgacggaca ggcagacaga
     2461 aatttcatat gtaataggat ggaagagcag ggatcagctt accctgtccg taaccgaaga
     2521 tttcgtcaga ctgagtcagg cattcgatcc tacaaacact tttggcgtca gactacctgc
     2581 cggaagcgaa tacagctgga aaaatgccgg tataaagttc gattccgata cacgtaattt
     2641 attatatttc agtgcggaag gcttatacgg aggatattat aacggaaaca ggctgttttt
     2701 aagcggctcc cttaattaca gggttgaacc ttacgggagt attgccatta ctgccaatta
     2761 taacgacatt aaactgcctg aaccgtataa tagtgcagaa cttatcctga taggaccacg
     2821 actggatatt acctttacca acaatctttt cctgaccact tttattcaat acaacaatca
     2881 gattgacaat ctcaatacca atatccgttt tcagtggcgt tttgcgccgg tttcggacct
     2941 gtttatagtc tataccggga atgcctttgc tgatgatccc atgatgaaca agaacagggg
     3001 gctggtagta aaattgtctt attggtttaa ctgaaccgtc aggaaataac tgacgcggct
     3061 gttattaacc attatagatg ggcaataacc gaacccgatg taatcaattg aaacggatgc
     3121 ttatagctga ctcggctgta attaacagaa aggtctg
//
LOCUS       DAOSPX010000358         3139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_27946_length_3139_cov_3.862192, whole genome shotgun sequence.
ACCESSION   DAOSPX010000358 DAOSPX010000000
VERSION     DAOSPX010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27946_length_3139_cov_3.862192"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1111
                     /locus_tag="PLZ75_13095"
     CDS             80..1111
                     /locus_tag="PLZ75_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOS73374.1"
                     /translation="MTDLRTQLKLFAYLYIIMLTILFFLVFILSMALATLGISLTIRL
                     RNTHKSETFGFLLYFQIFIYTFGFYGIWGQVLINAFLLPNIPGGIHPGFPNIPLLMGI
                     PFLIFAWLMLIQFSSSAAGRQKVRYFIPGFLVLNFALLFLLGYYVAGTDSPGTEPLIR
                     NYFIVMNVAYALLASAVISIPSGSRTLTRKKDIRSLALLLPLITVIQCLPLLFHETEP
                     RTGLIFIFIFFLGNIFIPVFLSYAALLPSVPGQTAGDLSFRDFCVKYEISPRESDVIK
                     EICKGLSNKEISDKLFISLQTVKDHTHRIYIKTNVRSRVQLINLVKGESGSPLTSAQD
                     RGLPGIKNS"
     gene            complement(1098..1721)
                     /locus_tag="PLZ75_13100"
     CDS             complement(1098..1721)
                     /locus_tag="PLZ75_13100"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOS73375.1"
                     /translation="MTISSKTVILADGSFPENEIPFGYLRNAERIVCCDGGADQLIRA
                     GFVPEAIVGDLDSISPEIAEKYSARLFPDRDQETNDLTKAVRWCTARGYRDLVILAAT
                     GKREDHTIGNISLLVEYAAEAEVMMVTDSGVFRPYLKSCVFSAFPGQQISVFSINPQT
                     EITSRGLVYPLEARKVKNWWEATLNEATGENVELEFEDGAIIVFSCF"
     gene            complement(1763..2422)
                     /locus_tag="PLZ75_13105"
     CDS             complement(1763..2422)
                     /locus_tag="PLZ75_13105"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOS73376.1"
                     /translation="MMIERKKLVLAGVIACFTACAGDVLTLFILAGKYPGYNQLFNTI
                     SSLGASESPVSSIISLWWVILGVLIISFAFGFRAAFSPGNKYITVAFWLLIIYGLGEG
                     IASGLFKADLVHNSMTLSYGIHDILGGAGVVCILVLPLVVQKVDQFSINPGFRKLSHV
                     VFVLGMLIQILFTFRFFGAGNNFPGKYTGLWQRLFLLMYYTYMIIIAIKMIVSMGRQE
                     K"
     gene            complement(2412..>3139)
                     /locus_tag="PLZ75_13110"
     CDS             complement(2412..>3139)
                     /locus_tag="PLZ75_13110"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOS73377.1"
                     /translation="YRVPEETLQIYKSLSRKCLFLGQEGVVDLGLFTLEGGKNKALRN
                     AINKVIDGGLKSSIHTPPIMDGLLQKLKTVSDEWLRSTRRNEIIFSQGMFDWNELKKQ
                     TIITVENPEGKVIAFLNIIPDYKQGEATYDLIRRTDDAPHGVLDFMLVELFRYLKSQN
                     YLTVNLGFAPLGGITDPHTFQERSMKFAYERLKSFAHFKGLRNYKEKFFPVWYNKYLI
                     YANDYDLLEIPAILAKVIQPNDD"
BASE COUNT          904 a          736 c          594 g          905 t
ORIGIN      
        1 ccggctcaat actatcgtag tgtttttcac catcactacc aaagtatgta tccctgcccg
       61 cggcctgatc tgaaaacaaa tgacagatct ccggacacag ctgaaattgt ttgcatatct
      121 ttatatcatc atgctgacca ttttgttttt cctggttttt atcctttcca tggcactggc
      181 aaccctggga atctctctca ccatacgact gagaaatact cacaaatcgg agacattcgg
      241 cttcctgctc tattttcaga tcttcatata cacatttgga ttttacggaa tatgggggca
      301 ggtactgata aatgcattcc ttctgccaaa cataccgggg gggatccatc cgggttttcc
      361 gaatattccc ttactgatgg ggatcccatt ccttattttt gcctggctga tgctgatcca
      421 gttctcatca tcagccgcag gccggcagaa agtgagatac tttattccgg gcttcctggt
      481 tctgaatttc gcactgttat ttcttcttgg atattatgtt gccggaacgg actcaccagg
      541 aacggaacca cttatcagaa actacttcat tgtcatgaat gtggcctatg ccctgcttgc
      601 ttcagccgtc atcagcatac cttccggaag caggacactg acacgtaaaa aggatatccg
      661 ctccctggca ttgttactgc cgctgataac agttatccag tgcctgcccc tgcttttcca
      721 tgaaacagaa ccccggacgg gactgatctt catttttatt tttttcctgg gaaatatatt
      781 tattcctgtt ttcctgagct atgctgcatt attgccatca gttcccggtc agaccgcagg
      841 agatctttcg ttcagggatt tctgtgttaa atatgaaata tcgccccgtg aatcggatgt
      901 gataaaagag atatgcaaag gtctgagcaa taaggaaata tcagacaaac tctttatttc
      961 ccttcagacg gtaaaagacc atacccaccg catttacatc aaaacgaatg tcaggagcag
     1021 ggtacagctc atcaatcttg taaaggggga aagcggatca ccactcactt ctgcccagga
     1081 ccgtggactg ccgggaatta aaaacagctg aaaactatta ttgccccatc ttcaaactca
     1141 agctcaacat tttcaccggt ggcttcgttc agtgtggctt cccaccagtt tttcactttc
     1201 cttgcttcaa gaggataaac gagtccgcgg gaagtgattt ccgtttgagg attaattgaa
     1261 aaaacagaaa tctgctggcc tggaaaggct gaaaacacac atgatttcag atatggccgg
     1321 aaaactcccg aatcagtgac catcatcact tctgcttccg cggcatactc aacaagaagc
     1381 gaaatattgc ctattgtatg atcttcacgc ttccccgtgg ctgccagtat caccagatcc
     1441 ctgtaccccc tcgcggtaca ccatctgact gccttggtaa gatcattcgt ttcctggtcc
     1501 ctgtcgggaa aaagccgggc actgtacttt tccgcgatct ccggagatat tgaatcaaga
     1561 tccccgacta tcgcttccgg aacaaatccg gcccttatca gctgatctgc ccccccgtcg
     1621 cagcaaacaa tcctttcagc atttctgaga taaccaaatg gaatctcatt ttcaggaaaa
     1681 ctaccgtcag caaggatgac ggtttttgaa gaaattgtca tggaaatatc ttttacatcc
     1741 cggatatggt tcaaatattg aattattttt cctgtctgcc cattgatact atcattttga
     1801 ttgcaataat tatcatgtac gtgtaataca ttagtaaaaa gagtctttgc cataatcctg
     1861 tatatttacc cgggaaatta ttgccggctc caaaaaaccg aaaggtaaat aatatctgaa
     1921 tcaacatgcc aagcacaaaa acaacgtgtg ataatttccg gaatcccggg ttaatggaga
     1981 actgatctac tttctgtacc acgagaggga gtacaagaat gcaaacaact ccggcccctc
     2041 caagtatatc atgaatacca taggacaaag tcatggaatt atgtaccaga tcggctttaa
     2101 acaatcctga ggctataccc tcacccaatc cgtaaattat taataaccaa aatgctacag
     2161 taatatattt attccccggt gaaaatgcag ctctgaaacc aaatgcaaaa gaaattatta
     2221 atacccccag aattacccac cataaggaaa ttatggaaga gacagggctt tcagatgcac
     2281 cgagcgagct tatcgtgtta aaaagctgat tatatccagg atatttacca gccagtataa
     2341 acaaagtcag aacatcaccg gcacaggctg taaagcaagc aattacacca gctaaaacta
     2401 gtttctttct ctcaatcatc atttggttgt atcacttttg caagaattgc cggtatctcg
     2461 agaagatcat aatcattggc gtatattaga tatttattat accatacagg gaaaaacttc
     2521 tctttataat tccttaaacc cttaaaatga gcaaaagatt ttaacctttc ataagcgaac
     2581 ttcattgatc tttcctgaaa agtatgtgga tctgtaattc cgcccagagg tgcaaaacct
     2641 aaatttactg tcaaataatt ctgtgatttc aggtatctga aaagttcaac aagcataaaa
     2701 tccagaaccc cgtgaggtgc atcatcagtt ctgcggataa ggtcatatgt agcttcaccc
     2761 tgtttatagt caggaataat attcagaaaa gcgatcactt ttccttccgg attttcaaca
     2821 gtaattatag tttgtttttt cagttcattc cagtcaaaca ttccctgtga gaaaataatt
     2881 tcattgcgcc gggtagatct aagccattca tctgacactg tttttagttt ttgcaatagc
     2941 ccgtccatga tggggggagt atgaattgaa gatttaagtc ctccatcgat aactttatta
     3001 atggcattgc gcagagcttt gttcttaccc ccctcaagtg tgaataaacc aagatctaca
     3061 actccctcct gcccgaggaa caggcatttt ctggacaggc ttttataaat ttggagggtt
     3121 tcttcaggca cgcggtaaa
//
LOCUS       DAOSPX010000359         3109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_28280_length_3109_cov_3.418795, whole genome shotgun sequence.
ACCESSION   DAOSPX010000359 DAOSPX010000000
VERSION     DAOSPX010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28280_length_3109_cov_3.418795"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLZ75_13115"
     CDS             <1..867
                     /locus_tag="PLZ75_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOS73378.1"
                     /translation="GLAAIDIDNHTAFHLEAVQTINSDDQSLSDWYAEVISERKDTLK
                     SISKYLVADAWFSKKSFVDQIISLDMHLVSRLRDDADLRYLSKAPLTGKRGRPRKYTG
                     KIDTSNIDRDYFTFISEDKESLIYATEVYSKALKRNILLVHVTYSKENGKDTRKLYFS
                     TDISMNPGDVLLYYRSRFQIEFLYRDGKQHTGLNDSQARSENKLHFQFNASLTSINIA
                     KAIHWLSIPIKEREAFSMADIKTMNHNILMMNRFFDVFGIYPYSAKTINMSENLYYTA
                     LWPLKTLTNYCK"
     gene            complement(847..1023)
                     /locus_tag="PLZ75_13120"
     CDS             complement(847..1023)
                     /locus_tag="PLZ75_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73379.1"
                     /translation="MIYSNIDPGKLIRQYDRTVEYLTRGDFASAEVKKMPGTTGYYRA
                     KLDQENHLLFTIVR"
     gene            1429..2202
                     /locus_tag="PLZ75_13125"
     CDS             1429..2202
                     /locus_tag="PLZ75_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS73380.1"
                     /translation="MKKVSRRDFIKTGSIAAAGISISAEKITGAGDQAWSVKVGVIGT
                     GYRGTSHVSNLLTIEGVEIIAVCDLFEDRAKNAASICKKNGRKPPAVYFGDMNVYKKM
                     LDKTDLDAVIIATYWDSHSSIAVHAMEHKIYPGIEVPAALTVDDTWKLVEISEKTGIP
                     CMMLENWSFRQDNLALLNIKRLGMLGEIVHCHCAHSHDCIDHWFFDSKTGFDRWPADY
                     LRKYNRDQYPTHTVGPVMSWLDINQRDAFEEIYSRASAS"
     gene            2305..2739
                     /locus_tag="PLZ75_13130"
     CDS             2305..2739
                     /locus_tag="PLZ75_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73381.1"
                     /translation="MGKTLVINYDMQLPRPFSNRWMLQGTKGVYYEEKPAIYLVDISS
                     EYHQWEPWKPYEEKYNHKWWHKDLSSQSHGGTDWIMLNQFINAVKGKGPTPIDVYDSA
                     VMTAIVELSGISIERNAPVAFPDFTKGKWQTNKPYFAMDKTV"
     gene            2814..>3109
                     /locus_tag="PLZ75_13135"
     CDS             2814..>3109
                     /locus_tag="PLZ75_13135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS73382.1"
                     /translation="MDQLREDSELIKKLQKGDVEAFDLIYEKYAGKLYLFGLKYLRTS
                     ADAEELVQSVFLKIWENHKQLNKELSFKSYLFTIAYNDICKLFRKRKYMQKFIT"
BASE COUNT         1000 a          620 c          627 g          862 t
ORIGIN      
        1 ggcttagctg ccattgatat tgataaccat actgcttttc atcttgaagc tgttcaaaca
       61 attaactctg atgatcaaag cctttctgat tggtatgcag aagtaatctc cgaaagaaag
      121 gataccttaa aatcaatatc aaagtacctg gttgccgatg catggttctc caaaaagtct
      181 ttcgtggacc agattatttc tttagatatg catctggtca gcagactaag ggatgatgct
      241 gaccttagat atctcagcaa agccccgctc acggggaaac gaggcagacc aagaaaatat
      301 accgggaaaa tagataccag caacattgac agagactact ttacttttat ctcagaagat
      361 aaggagtcac tcatatacgc aacagaagtc tattccaaag ctcttaaacg taacattctt
      421 ctggttcatg tcacatattc aaaagaaaat gggaaagata cccggaagct ctatttttct
      481 accgacatct caatgaatcc tggtgatgtg ctgctttatt atcgtagccg cttccagata
      541 gaatttcttt atcgcgatgg gaaacaacat accggtctga atgactcaca agccagaagt
      601 gagaacaaac tccactttca attcaatgcc tctctcactt cgattaacat tgccaaggca
      661 atccattggt taagcatacc aataaaagaa agagaagcat tctcaatggc tgatattaaa
      721 acaatgaatc acaacatctt aatgatgaac cgattttttg acgtgtttgg tatttaccca
      781 tactctgcaa aaacaataaa tatgtcagag aacttatatt atacggcact atggccgctt
      841 aaaactttaa cgaactattg taaataacag gtgattctcc tgatcaagtt tagctctgta
      901 atatccggtg gttccgggca tttttttcac ttcagccgat gcaaaatcac ctcttgtaag
      961 gtattctacc gtcctgtcat actgtctgat aagtttcccg ggatcaatat tgctgtaaat
     1021 caaagtgtta tatatcttct ggtgcttaat taagaaatat cacgcaattt atcaaaaacc
     1081 ttaaaaacgg tcaaactata attgttccgg aaatcaaaca aggtgaataa tccggaaatt
     1141 aagttcctgg caccagggca catacgagac actaagtttt accaggtaca agtaattact
     1201 tctttatgga tctactatat aactcactca taaatccccc aggatggact tttgaatctg
     1261 tacgcccccc ttagggcaag gggtggcaag aagggcagga gttattatag ttgatgttcc
     1321 tgagttctct aagatggtac gcttagtggc atacaaaatt ttccggttat ttaaacagac
     1381 taaaaacaat gaatttattt ttatacctta ccatcagatt taaacataat gaaaaaggta
     1441 tcaagaagag atttcattaa aaccggatcg attgctgcag ccgggattag catatcagca
     1501 gaaaagataa ccggcgccgg tgatcaggca tggtccgtta aggtcggagt gatcggtacc
     1561 ggatacaggg gaacatctca tgtaagtaat ctgctgacca ttgagggagt tgaaattatt
     1621 gccgtatgtg atctttttga agacagggca aagaacgccg cctccatctg taagaagaac
     1681 ggaaggaaac ctccggctgt atattttgga gatatgaacg tctacaaaaa gatgctggac
     1741 aagacagacc ttgatgccgt aataattgcc acatactggg attcccattc ctcaattgct
     1801 gtccatgcaa tggaacataa aatatatcct ggcatcgaag taccggcagc attgactgtt
     1861 gacgatacct ggaaactggt tgagatatcc gaaaagaccg ggatcccgtg catgatgctc
     1921 gaaaactgga gtttcaggca ggacaatctg gcgcttctta acataaaacg tttagggatg
     1981 ttaggagaaa tcgttcattg tcattgtgcc cattcccacg actgtatcga tcactggttc
     2041 tttgacagta aaaccggatt tgacagatgg cctgcagatt acctccgcaa atataacaga
     2101 gatcaatatc ctactcatac agtgggtccg gtcatgagct ggctggatat taaccagcgc
     2161 gatgcttttg aagagatata ttctagggcc tcggcatcat aagggataaa tgcatacttt
     2221 aaaagaaagt ttggtaatga tcatcccaat gccagcctca cttataaaca gggagacata
     2281 gtaacttcaa ctctcaaaac aaagatgggt aaaaccctgg ttatcaatta tgatatgcaa
     2341 ttaccacgcc cattttcgaa ccgttggatg ctgcagggca caaagggagt ctattatgag
     2401 gaaaaacccg cgatttacct ggtcgatatc agttctgagt accatcagtg ggaaccatgg
     2461 aaaccttacg aggagaaata caatcacaag tggtggcata aagatctttc atcccaatct
     2521 catggaggca ctgactggat tatgcttaac cagttcatta atgccgttaa gggaaagggc
     2581 cccactccca tcgatgttta tgattctgct gtcatgactg ctattgtcga attatcagga
     2641 atatcaattg aaagaaatgc cccggttgca tttcccgatt ttacaaaagg caaatggcag
     2701 acgaataaac catattttgc gatggataag acagtctgat ttcaatcgga tttacagggt
     2761 tattgcatgg atattcacat gaggattttg actattttta cacaaaaaac atattggatc
     2821 agttacggga agatagtgaa cttattaaaa agcttcagaa aggagatgtg gaagccttcg
     2881 accttatata tgaaaaatat gcgggaaaac tgtatttatt cggattgaaa tatctccgga
     2941 cttctgccga tgctgaagaa ctggtacagt ctgtctttct taaaatctgg gagaatcata
     3001 aacaacttaa caaggagtta tcgttcaaat cctacctttt taccattgca tacaatgata
     3061 tctgcaagct tttccggaaa aggaaatata tgcagaagtt cataacaga
//
LOCUS       DAOSPX010000360         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_28383_length_3101_cov_3.813198, whole genome shotgun sequence.
ACCESSION   DAOSPX010000360 DAOSPX010000000
VERSION     DAOSPX010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28383_length_3101_cov_3.813198"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLZ75_13140"
     CDS             <1..294
                     /locus_tag="PLZ75_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOS73383.1"
                     /translation="YNSLPRPDIFVYLHLDTGRLLKNIENRGRPYERSITEQYLKGIQ
                     DSYFSFFRQNPENKYLVIDINKLDFVSNNDDYKKIRDAIFMKEYPAGMNRLIL"
     gene            487..2199
                     /locus_tag="PLZ75_13145"
     CDS             487..2199
                     /locus_tag="PLZ75_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73384.1"
                     /translation="MKKISSYFILFLAAAVMTGCSGLNKMKKNQDTIRYEVTPKVLEA
                     HGGIVGLTIKGEFPEKYFDKKATLTATPVLVYDGGETPYEKVQVLQGEKVMANNQVIT
                     YSGGSFTYNDIVPFKEEMRVAQLMLRVEASRGAKTVVFEPVKLADGVISTSTLVQKHG
                     RAVIMKDNFQRIIPESKVADIHYVINRSDIRSSELKGADINELRAYIKEVDADPDRRF
                     TTTVVSSYASPDGPITLNEKLSVNRGSAADRFIKREFTKIEAAKSGDFFDNITTPEDW
                     EGFRAAVENSSIRDKDLILRVLSMYSDPVVREREIKNMSSAYEALKDEILPKLRRSVM
                     TVNVDKIGRTDEQILALARSNPGELSVEEILYAPTLVTDLNEQLRFYQAAAQTYPKCI
                     RAHNNIGHTLLALGRTDEAIRAFEAAKAINYNDEVKNNLAAAALVKGELVNAEELYNS
                     MTTQTPESRFGLGVIAITKGEYDNAVNLLGTAPCFNLALAQLLKGDVNRAKATLDAMT
                     ETCNGKAPYLKAVVGARLDDRNYMLAGLREAVGINPAFKAMAKTDMEMAKFFADDTFR
                     SLVQ"
     gene            complement(2349..2885)
                     /locus_tag="PLZ75_13150"
     CDS             complement(2349..2885)
                     /locus_tag="PLZ75_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOS73385.1"
                     /translation="MRKLRNSELGRKSVEQFRKAGKSPLVIVLDNVRSLSNVGSIFRT
                     ADAFLAECIYLCGITGVPPHPEIHKTALGATESVAWKYFDNTPAAIALLKSSGYRIIG
                     IEQTEGAIYLSDFSVEKDIKYALVFGHEINGVDQAVIDECDGVIEIPQSGTKHSFNIA
                     VSAGIVLWEFHKQHHSYR"
BASE COUNT          934 a          678 c          685 g          804 t
ORIGIN      
        1 tacaactccc tcccgcgtcc cgatatcttt gtgtatcttc atcttgatac cggaaggctt
       61 ctcaagaata ttgaaaacag gggaagaccg tatgaaaggt ccattactga acaatacctg
      121 aaaggcattc aggacagcta tttttccttt ttcaggcaga atcctgaaaa caaatatctt
      181 gtcattgata tcaacaaact ggattttgtt tcgaataacg atgactataa aaaaatccgt
      241 gatgcaatct tcatgaagga atatccggca gggatgaaca ggcttatttt gtagctccca
      301 tttccgggga aagagtgatt tgtcagaagg attgattcac tcaggattca tataatcatt
      361 aaaacggaat tattttataa ttaaatattt tactgtacct gaagtttttt ttacatttgc
      421 agtttaattt gtatattgct caaatattta taaacgacta ttatcaaaca aataaccagt
      481 tttatcatga aaaaaatcag cagctatttt attctttttc tggccgcagc agtaatgaca
      541 ggctgcagtg gcctgaacaa aatgaagaaa aatcaggata ccatcaggta tgaggttaca
      601 ccgaaagttc ttgaagccca tgggggtatt gtaggcctta ccatcaaagg cgagtttccg
      661 gagaaatatt ttgataagaa ggctacgctc actgccactc ctgtcctcgt ttatgatggt
      721 ggtgaaactc catatgaaaa agtgcaggta ctgcagggtg aaaaggttat ggccaataat
      781 caggtaatca catattccgg tggtagtttc acttataatg acattgtccc cttcaaggaa
      841 gagatgaggg tggcgcaact gatgttaagg gttgaggcca gcaggggtgc aaaaaccgtt
      901 gtttttgagc cggttaagct tgctgatggg gttatttcaa catcaaccct ggtccagaaa
      961 catggcagag cggttattat gaaagataat ttccagagga ttattcctga gagcaaagta
     1021 gctgatatac attatgtgat caacaggtca gatatccgca gctcagagtt aaagggtgct
     1081 gacataaatg aactgagagc atatatcaag gaagtggatg ctgatcccga ccgccgcttc
     1141 acaacaacag ttgtcagttc ctatgcatct cctgatggac ccatcacact gaatgaaaaa
     1201 ctgtcagtca acagaggttc agctgccgac agattcatca agagggaatt taccaagatt
     1261 gaagcagcaa aatcaggtga tttctttgat aatatcacca cacccgaaga ctgggaagga
     1321 ttcagggctg cagtcgaaaa ttcctcaata cgcgacaagg atctgatact ccgtgttctt
     1381 tcaatgtatt ccgatcccgt tgtccgtgaa agggaaatca agaacatgtc ttcagcctac
     1441 gaagcactca aggatgaaat tcttccgaaa ctcagaagat cagttatgac tgtcaatgtt
     1501 gacaagatcg gccgtaccga tgaacagatc cttgcactgg ccagatccaa tccgggagaa
     1561 ctgagtgttg aggaaatact gtacgccccc acacttgtga cagatcttaa cgaacagctg
     1621 agattctacc aggcagcagc acagacatat ccgaaatgca tcagggcaca taacaatatc
     1681 ggacacaccc ttctggcact cggaagaacg gatgaagcaa tcagggcttt cgaagcagca
     1741 aaggcaatca attacaacga tgaggtcaag aacaacctcg cagcagcagc tcttgtaaag
     1801 ggtgaactcg ttaatgccga agaactgtac aattcaatga ctacccagac acccgaatca
     1861 agatttggtc tcggagtgat tgcaataaca aagggtgaat atgacaatgc ggttaacctg
     1921 ctgggcacag caccctgttt caatctggca cttgcacagt tgctcaaggg agatgtaaac
     1981 agggccaagg caaccctgga tgccatgacc gaaacatgca acggaaaggc tccctatctc
     2041 aaggcagttg tgggtgcaag acttgatgac aggaattata tgctcgccgg ccttcgtgaa
     2101 gccgtgggaa ttaatcccgc attcaaggcc atggcaaaaa ccgacatgga aatggccaag
     2161 ttctttgccg atgatacatt cagatcactg gttcagtaat acaataagat atattgcata
     2221 aataaaagag gctgtccggg aacgggcagc ctcttgcgtt ttatatccgg cacccggctc
     2281 aggaacagga taagtatcgt gaaaaataca ggccagctgt cgtggccgga atataaaagc
     2341 cgccccattc atctgtaact gtggtgctgt ttatggaact cccacagaac gattcccgca
     2401 cttaccgcta tattgaagga atgctttgta cctgactggg gtatttctat cacaccgtca
     2461 cattcatcaa tcactgcctg atccacgcca ttgatttcat gaccaaatac aagagcatat
     2521 tttatatcct tttccacact gaaatcgctg agatatatgg cgccttcagt ctgttctatc
     2581 ccgataatcc tgtatcctga tgatttaagc agtgcaatgg ctgcgggtgt attgtcaaaa
     2641 tatttccatg ccaccgattc agtggcgccc agtgccgtct tatgtatctc cggatgaggc
     2701 gggacgcccg taatgccgca cagataaata cattcagcaa ggaaggcatc tgctgtcctg
     2761 aatattgacc ctacattgct caggcttctg acattgtcca gaacaattac aagcggcgat
     2821 ttgccggctt tcctgaattg ctccacactt tttctgccaa gttcactgtt ccgaagcttc
     2881 cgcattattt ttttcgctaa tttagaaaaa atatactttg atagtcctgt ttcacaggag
     2941 agtgaaacca gtcaaaacgg aaggaaatga atattcatga ataccaggcc aaatcgctgc
     3001 tgagtgcatt tggagccggc gtacaggaag gctccaaatg cactcagcag cgatttggcc
     3061 tggtattcat gaatattcat gaataccagg ccaaatcgct g
//
LOCUS       DAOSPX010000361         3093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_28497_length_3093_cov_3.510204, whole genome shotgun sequence.
ACCESSION   DAOSPX010000361 DAOSPX010000000
VERSION     DAOSPX010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28497_length_3093_cov_3.510204"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="PLZ75_13155"
     CDS             complement(<1..1440)
                     /locus_tag="PLZ75_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HOS73386.1"
                     /translation="MKKNLLICGSILLLFVFAHNLRSQGVKQVKLTDFSLQSSVLVDE
                     TGEMISSPAFNPDIYWFPVKVPCTVLTGLTANNIYPDPYSGLNNMLIPDASDEFNRMY
                     GLEKYSHLPGHPNPWKKPYWYRTKFHVPEEDRGRHFQLIFKGINYRAAVWVNGIQIAD
                     SAEMAGMFSQYSLDISKHVRAGEENGLAVKIYPLDYPGLPDKEQLEALGDFYLNGGPT
                     GDIGKNVTMLCSVGWDWIPPVRDRNMGIWLPVYLRTSGGVTFSNPRLVTDLPDLPDTS
                     LAGISLNLRLVNHTAVNTGGTLHVKIAPENFTGRSVEFTKPVSINSGESVMIDLNPAN
                     TSELNIRNPALWWPNGHGRPDLYRLRLQYSDHNGIVNDTSFIFGIRTVATKAVSLKNN
                     WRRDFYVNGRRIHLTGGAWVPDMMLNRDSLRYDYELRLCRNAKINLVRIWGGGVTPCD
                     EFFEAADRYGLLVWSDFWITGDTQGEFKGS"
     gene            1572..2453
                     /locus_tag="PLZ75_13160"
     CDS             1572..2453
                     /locus_tag="PLZ75_13160"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOS73387.1"
                     /translation="MSETLGKWLASIIPWLFDHGIKILIIAAGSWILYKIICRIIIRT
                     VRLAVVRDKQMPAEAETKREDTLIRIFTGATRVIMLILAILMMLQEAGLKIGPILAGA
                     GIAGIAVGFGGQYLIKDVITGLFIILENQYRIGDVVNIDGTSGTVQDISLRKVTLRDI
                     DGIVHHINHGSVERVSNKSKDFARVHLDIEVAYDTNVEHLIEVINRTCRELAEDPAFR
                     ESIIEAPRFLRITDFAQSAVKVKILGDTIPLKQWEIGGELRKRLKIAFDREGIKMSVP
                     QRVVSQVNLISNDNNQQ"
     gene            2434..>3093
                     /locus_tag="PLZ75_13165"
     CDS             2434..>3093
                     /locus_tag="PLZ75_13165"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73388.1"
                     /translation="MTITSNKRFGSIPVFFTAISTILGAILFLRFGFAVGTLGFWGVL
                     ILILLGHALTIPTAFALSELATNKRVEGGGEYFIISRSFGLNIGATIGFALFLSQAIS
                     IAFYIIAFTEAFEFIFNYFMNSFNIYIPRQVISIPATLILGYIILKRGANMGMKMLYF
                     VVAILFLSLLLFFLGKTEYGTTMGSGIPAGDLRNMKDFFIVFAIVFPAFTGMTAGVGL
                     SG"
BASE COUNT          827 a          728 c          722 g          816 t
ORIGIN      
        1 ggagcctttg aattctcctt gcgtatcgcc cgtaatccag aaatccgacc ataccagcaa
       61 tccgtaacgg tcggccgctt caaagaattc atcacaggga gttacccctc cgccccatat
      121 cctcaccagg ttgattttcg cattccggca caggcgtaat tcataatcat acctgagaga
      181 atccctgttg agcatcatgt cgggaaccca ggccccgccg gtaagatgaa ttctcctgcc
      241 gttcacataa aaatcacgcc gccagttatt cttcaggctg accgcctttg tcgccacagt
      301 cctgatgccg aaaatgaatg aagtgtcgtt gacaatcccg ttatgatccg aatactgcag
      361 cctgaggcgg taaagatccg gacgcccgtg gccattcggc caccacagtg cggggttcct
      421 gatattaagc tcactggtgt tggccggatt cagatcaatc atcactgatt cacctgaatt
      481 tattgatacg ggttttgtga attcaacaga tctgcccgtg aagttttcag gagcaatttt
      541 cacatgaagg gtaccccccg tatttacagc cgtatggttg acaagcctta gattcaggga
      601 gatcccggcc agactcgtat ccggcaaatc cggaagatcc gtcacgagcc ggggattact
      661 gaatgtgact ccccccgagg tccgcagata aacgggaagc cagataccca tgttcctgtc
      721 ccgcacgggc ggtatccagt cccatcccac cgaacacagc atggtgacgt ttttgccaat
      781 gtcgccggtg gggccaccgt tcaggtaaaa atcccccagt gcctcaagct gttccttgtc
      841 aggcagtccc ggatagtcaa gaggatatat cttaaccgca agtccgtttt cctcgcccgc
      901 ccttacatgc ttcgaaatat caaggctgta ctgggaaaac attccggcca tttccgcgga
      961 atctgctatc tggatgccat tgacccatac ggcagccctg taatttatac ccttgaaaat
     1021 cagctggaaa tggcgtcccc tgtcttcttc aggcacatgg aacttcgtcc ggtaccagta
     1081 gggcttcttc cacggattgg gatggccggg aagatgactg tacttttcaa ggccgtacat
     1141 cctgttgaac tcatccgatg catcaggtat aagcatattg tttagacctg aataggggtc
     1201 aggatatatg ttgtttgcag tcaggcccgt cagtacagta catgggacct tcacgggaaa
     1261 ccagtaaata tccggattga aagccgggga ggatatcatc tctcccgttt catccaccag
     1321 aacagatgac tgaaggctga aatcagtaag ctttacctgc tttaccccct gtgaccggag
     1381 attgtgagca aaaacaaaaa gcagcaaaat actgccgcag atcagcaagt tttttttcat
     1441 gatttttgaa ttaaggctta tgttttgcat gctttgctaa attaattata tatgccaaag
     1501 taaattctgt tcagcatgta atattttcaa aaaagccctt aatttgcaga atttatttat
     1561 ctgaaatcag aatgagtgaa actttgggta aatggctggc ctctatcata ccgtggcttt
     1621 ttgaccatgg cataaagatt ttaataatag cggcaggttc ctggattctg tataaaatta
     1681 tctgcaggat aatcatcagg actgtcaggc tggcggttgt acgcgacaag caaatgccgg
     1741 cagaagccga gacaaaacgc gaagacacgc ttataaggat atttaccggg gcaacaagag
     1801 tgatcatgct aattcttgca attctgatga tgctccagga agccggtctg aaaatcggtc
     1861 ccatcctggc cggggcagga attgccggta tagctgtggg attcggcgga caatatctta
     1921 taaaggatgt cattaccgga ttattcatta tccttgaaaa ccagtaccgg atcggtgatg
     1981 tagtaaatat agacgggaca tccggaacag tacaggatat cagtctgaga aaagtcaccc
     2041 tcagggatat tgacggtatc gttcaccata tcaaccatgg ctccgtggaa cgcgtatcaa
     2101 ataagtcaaa ggactttgcc agagttcatc tcgatattga agttgcgtat gacacgaatg
     2161 ttgaacatct cattgaggta ataaacagaa catgcaggga acttgcagag gatcctgcat
     2221 tcagggaatc cattatcgaa gcacccaggt tcctgcggat aactgatttt gcccagtctg
     2281 ctgtcaaggt aaaaatactg ggagatacca tacctctgaa gcaatgggaa ataggaggtg
     2341 aattacggaa gcggctgaag atagccttcg acagggaagg tataaaaatg tctgttccac
     2401 aaagggttgt aagccaggtt aatcttatca gcaatgacaa taaccagcaa taaacgcttt
     2461 ggttccattc cggttttctt tacggcaata tccaccatac ttggagcaat cctctttctg
     2521 agattcgggt ttgcggtggg aacactgggg ttctggggag tcctgattct gatcctcctg
     2581 gggcatgccc tgactatacc aaccgcattc gccctctctg aactggcaac gaacaagagg
     2641 gttgaaggag gcggggaata tttcattata tcccggtcat tcggactgaa cataggggca
     2701 accataggat ttgccctttt cctgtcacag gctatcagta tagcattcta catcatcgca
     2761 ttcacagaag cttttgaatt catattcaat tattttatga attcattcaa catttatatt
     2821 ccaagacagg taatcagtat acccgcaaca ctgatactgg gatatataat cctcaaaagg
     2881 ggtgcgaaca tgggaatgaa aatgctctac ttcgtggttg caatactgtt tctgtcatta
     2941 ttgctgtttt tcctggggaa aacggaatac ggtacaacca tgggttccgg tattcctgcc
     3001 ggcgacctga gaaatatgaa ggacttcttc attgtatttg ccattgtgtt tcctgcattc
     3061 acgggtatga ctgccggtgt gggcctctcc ggt
//
LOCUS       DAOSPX010000362         3078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_28686_length_3078_cov_4.495203, whole genome shotgun sequence.
ACCESSION   DAOSPX010000362 DAOSPX010000000
VERSION     DAOSPX010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28686_length_3078_cov_4.495203"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLZ75_13170"
     CDS             complement(<1..1092)
                     /locus_tag="PLZ75_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73389.1"
                     /translation="MAISNLTVTGAGGNPPSQILVEGEVTGCEIVQVVSSCTANPVQI
                     NIPTGGTHNWSAILSNDKGCECGEIIKVVAWCGLGQPGSSAVTLDMQLTCGNCPSVNV
                     TADPPGPCVNGKRSVTFHATAGNVPSAGVAMQWKFPNSNMPFGPAFTVTNNGALADQT
                     VEYPVGASGPVTYTATLDVILPSGCMPVDVPVTIDPCKGEPPLCCPDVTLLQPAISGC
                     APGSAKADFTATLTWPEDDCTKTVPSSYLWTLNGSNNKKYRRSTSQPNTDTSTPWTDV
                     STGNPSAVQFVAGGSYSIAVVAIIPGIDLLSCMPTDTRSFIIPACCPQLTGPLNASQK
                     PDDPCTWIFSAQVSNPNNAAVSFEWSFHDG"
     gene            complement(1226..>3078)
                     /locus_tag="PLZ75_13175"
     CDS             complement(1226..>3078)
                     /locus_tag="PLZ75_13175"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOS73390.1"
                     /translation="LISGLLAGKDHDALGEIAEKKLLWVPEDLFAGTEILSKIFVHPS
                     SPKQMSDYLRELLIIISSGNYAAEAEEIVETPAETPETAESIKATKADTAEQKGEKAR
                     QRITDEFIYRTLLSLNRIDAAAENTGIKPALNTWIRILDRILSKQSLPFTGEPLSGIQ
                     VMGILESRTLDFKNIIMLSVNEGVLPAVSTSSSFIPFSLREAFGLPSVNHQESIYAYH
                     FFRLLHRSENVTFIYNSDSEGLKSGEISRFLQQMKYGGGPVPEISGHSFSIQSLKPLS
                     ETVERTEEHTRRLVERFCGPSVIAGTSGTSGTSVIDDKGRSKTKTETGRKYYISPSAI
                     NTWLHCRMKFYYRYVNDLREKEVITEDIDPAMLGTLLHESIRNLYEKYAGRVLDPDET
                     EAMLRNRQLIESAIDKAIEENFRSENDFPVSGNELIIREVLVVFVKRILQRDKMYAPL
                     TILSFEDFHDFRIEVNACHEMSLLTGGRIDRIDMKQGIVRIIDYKTGIVRDAIASTEE
                     LFEEDRNRDLDGWLQTLIYCEAYLSKNPDAGLFPAIYKIRKDPGKDPSEMLRLKPDRS
                     VNDYHIIREEFLTGLKILLEKIFSNSEAFTMTEKAWSKCTYCPYRTLCQR"
BASE COUNT          765 a          728 c          697 g          888 t
ORIGIN      
        1 tccatcatga aatgaccact cgaaacttac agcggcatta tttggattgg atacctgtgc
       61 cgagaaaatc caggtacagg gatcatccgg tttttgcgac gcatttagcg gacctgtcag
      121 ctgggggcag caggcaggta tgatgaatga cctggtgtcg gttggcatgc aggataacag
      181 atcaattccg ggaataatgg caacaacggc tatgctgtaa ctgcctccgg caacgaactg
      241 taccgcagac ggatttccgg tggatacatc agtccagggg gtacttgtat ctgtattcgg
      301 ctggctggtg gatcgcctgt actttttgtt attagaaccg ttgagtgtcc acaaataaga
      361 ggaagggact gtttttgtac aatcatcttc cggccaggtc agggtcgccg taaaatcagc
      421 ttttgcagaa cccggtgcac atccggaaat ggcaggctgt aatagtgtta catccggaca
      481 gcataacgga ggttcgccct tacatggatc gatagttaca ggaacatcta ccggcataca
      541 gcctgatggc agaatgacat ccagagtggc agtataagtc acaggacccg atgcacctac
      601 aggatattct acagtctgat ccgccagggc cccattgttt gtgactgtga aagccggtcc
      661 gaaaggcata ttactattgg gaaatttcca ttgcatagca acccctgctg acggaacatt
      721 gccggcagta gcatgaaaag tgacactcct ttttccgttg acacacggtc cgggaggatc
      781 tgcagtaaca ttcactgaag ggcaattacc gcaagtaagc tgcatatcaa gggtgacagc
      841 cgaagaacca ggctgtccca ggccgcacca ggccactact tttataatct ctccgcattc
      901 gcagcctttg tcgtttgaaa gaatagctga ccagttgtgt gttcctccgg tcggaatgtt
      961 tatctgaacc ggatttgcgg tacagctgga aacaacctga acaatttcgc acccggttac
     1021 ttcaccttcc actaatatct gtgaaggcgg gttgccaccg gctcccgtta cagttagatt
     1081 gctgattgcc atagtaatct tgatttaagt tttacatatg gtctgccgca tattaaatgg
     1141 tgattattaa tctacgatag atcctgatta attgtatata taaacaaatg aggaaaaatg
     1201 tttgttcggg atgcggatga ttttttcatc tctggcataa agtccggtac gggcagtaag
     1261 tgcatttact ccaggctttt tcagtcatgg taaatgcttc cgaattgctg aatattttct
     1321 ccagcaatat tttcagaccg gtaagaaatt cttctcttat tatatggtaa tcattaactg
     1381 aacgatcagg ttttagccgc agcatttcag agggatcctt gcccggatct ttcctgattt
     1441 tgtaaatagc cgggaaaagt cctgcatcag gatttttaga aagatacgcc tcgcaataga
     1501 tcagtgtctg aagccagccg tcaagatccc tgttcctgtc ttcttcgaaa agctcttccg
     1561 tggaagctat tgcatccctg acaatgccgg tcttgtagtc aattatcctg acaattccct
     1621 gcttcatgtc aatcctgtca atcctgcctc cggttaaaag gctcatttca tggcaggcat
     1681 tcacttcaat cctgaagtca tgaaaatctt caaacgacag gattgtcagc ggagcataca
     1741 ttttatccct ttgcaggatc cgtttaacga aaaccaccaa tacttccctt atgatcaatt
     1801 cgtttcccga tacaggaaaa tcattttcag atctgaaatt ttcttctatt gccttgtcaa
     1861 tggcactttc aataagctgc ctgttcctga gcattgcttc tgtttcatca gggtcaagaa
     1921 cccttcccgc atatttttcg tagagattgc gtatcgattc atgcagcaat gttccgagca
     1981 tggcaggatc aatgtcttca gtaatcactt ccttttcccg gaggtcgttc acatacctgt
     2041 agtaaaactt catcctgcag tgcagccagg tattgatggc tgacggggaa atataatact
     2101 ttctcccggt ctcggttttg gtcttgctcc tgcccttatc atcaataacg gaagttccgg
     2161 aagttccgga agttccagca attactgacg gtccgcaaaa gcgttcaaca agccgccggg
     2221 tatgttcctc tgttctttca actgtctcac tgagtggttt caggctttga atactgaagc
     2281 tgtgaccgct tatttccggt accggtccgc cgccatattt catctgctgg aggaagcggc
     2341 ttatttcgcc gcttttcaga ccctcggaat cagaattata aatgaatgtc acgttttccg
     2401 acctgtgcag cagcctgaaa aaatgatagg cgtatattga ctcctggtga tttacggatg
     2461 gcaggccaaa agcctccctg agactgaagg gaatgaatga tgaggaagtg ctgactgccg
     2521 gaagcacccc ttcatttact gacagcatga taatattttt gaaatcaagt gtccgtgatt
     2581 caagtattcc cataacctgg ataccggaga gaggctcccc ggtgaaaggc agcgactgtt
     2641 tgctgagtat cctgtcaagt atccttatcc aggtattcag agccggtttt atccccgtat
     2701 tttccgcagc tgcgtctata cggttaaggg aaagaagagt acggtaaatg aattcatcag
     2761 tgatacgctg tcttgctttt tcacccttct gttctgctgt gtccgccttc gttgctttta
     2821 tagattctgc tgtttccggg gtttctgctg gtgtttcgac tatctcttct gcctctgctg
     2881 cataattccc ggaactgatg attatcagca attccctcag gtaatctgac atttgcttcg
     2941 gcgaggaagg atgcacaaat atttttgaaa gaatttccgt accggcaaac agatcttccg
     3001 gaacccacag gagctttttc tcagctattt ctcccagcgc atcgtgatcc ttacctgcca
     3061 gcagtcctga aatcagat
//
LOCUS       DAOSPX010000363         3074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_28743_length_3074_cov_4.120901, whole genome shotgun sequence.
ACCESSION   DAOSPX010000363 DAOSPX010000000
VERSION     DAOSPX010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28743_length_3074_cov_4.120901"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLZ75_13180"
     CDS             complement(<1..472)
                     /locus_tag="PLZ75_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73391.1"
                     /translation="MKKHGNHSPNITGFIFMLALLALLAGSCGTMQTVIDPKDLSYLY
                     NPTKNPINPGYSVSNESENLSYLAVKFFTAELYFSEANPQGIAMSQLLVTVKLYDVSR
                     GRVLQDTAYYNLNIIREEGRRDFVYKIPLRVRPGLDYMAEVRILDRLRQRVVQSF"
     gene            complement(488..1261)
                     /locus_tag="PLZ75_13185"
     CDS             complement(488..1261)
                     /locus_tag="PLZ75_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOS73392.1"
                     /translation="MKMNNMTIPHFEREFRVHVYETFVDEKANICALFNYMQDIAAEH
                     AIRLGYGRDDLMKENNFWVLSRMYAEISEYPFRDERITVKTWPFGTDNVFALRFFDLK
                     FPDGRTIAKASSSWLIIDAETKRIRRPDQTLEQLSSYRKSHEMPVRIAEKIRETPAGG
                     KVSDRFRVKVSDLDVNMHTNNVNYLKWTIDSYDFDFMLKHFPVSVEINYLAESVLGDN
                     ITIRTSGEGESTCAFTHSIMKDEAKELCRIRIEWKERKN"
     gene            complement(1248..>3074)
                     /locus_tag="PLZ75_13190"
     CDS             complement(1248..>3074)
                     /locus_tag="PLZ75_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOS73393.1"
                     /translation="LKIIAGLQSPTSGQVDMSREVTIGYLPQQMKLDDTTTVLNEVIK
                     AFPEITGLEREIESCTAEIASRKDYESPEYLKLCENLHLVEEKFRYAGGDNYLADSEK
                     ILRGLGFEDRDFDRPTGELSGGWRMRIELAKILLQNPSLLLLDEPTNHIDIESIQWLE
                     GFLASYRGAVILVSHDRAFLDNVTTRTIEISLGKIYDYKVSWSEFAVLRKERREQQLA
                     AFRNQQKMIDDTEKFIERFRYKATKAIQVQSRIKQLDRIERIEVDEEDFSEISLRFPP
                     APRSGTVVVDARELSKNYGTLNVLNRIDFRIHRGEKVAFVGRNGEGKTTFSKIIIGEL
                     DHEGLLKTGHNVRTGYFAQNLDELLNEEKTVFETVDDVARGEIRTKIRDMLGAFLFRG
                     DDADKKVKVLSGGERSRLALVRLLLEPHNLLVLDEPTNHLDMRSKDILKQALISYDGT
                     LIVVSHDRDFLDGIVSKVYEFRNHAIREHIGGIYDFLRKRKIENLREIERKEAAPLQR
                     KEDRENGNISSGKRKYLERKDYERNLRRLRSRLEKSESMIENLEKEISRIDTLMNSPG
                     HLAEATREFYEKYRELKERHAEEMDRWAQYTHELEVFLNEDE"
BASE COUNT          676 a          766 c          658 g          974 t
ORIGIN      
        1 caaaggactg cacgaccctt tgcctgagtc tgtcaagaat tctgacttct gccatgtaat
       61 caagcccggg cctcacccgg agcggaattt tataaacgaa atcccttctt ccttcttccc
      121 tgataatatt gaggttataa taggcagtat cctgaagcac ccttcccctg ctcacgtcgt
      181 aaagttttac cgtgacaagc agctgtgaca ttgctatgcc ctgggggttg gcttctgaga
      241 aatagagttc ggcagtaaag aatttcaccg cgagatagga aaggttttcc gattcgttgg
      301 atacgctgta tccgggattg atgggatttt ttgtcgggtt gtagagatat gaaaggtctt
      361 taggatcaat tacagtctgc atggtaccgc atgagcctgc aagaagagca agcagtgcca
      421 gcatgaatat gaatcctgtg atattagggc tatggttacc atgctttttc atgactgtca
      481 gagattatca gtttttacgt tctttccatt caattcttat cctgcacagt tcctttgcct
      541 catctttcat gattgaatgc gtgaatgcgc aggtgctttc tccctcccct gaagttctga
      601 tagtaatatt gtcaccaaga actgattcgg caagatagtt tatttccact gaaacaggaa
      661 aatgcttcag cataaaatca aagtcatagg agtctattgt ccatttaaga taattcacat
      721 tgtttgtatg catgttgaca tcaaggtcgg agactttcac cctgaatctg tcagacactt
      781 tgccgccggc cggggtttcc ctgattttct ccgcaattct gacaggcatt tcgtgagatt
      841 tcctgtatga cgacaattgt tccagtgtct ggtccggccg ccggatcctc tttgtttcag
      901 catcaattat cagccatgag gaagatgctt ttgcaatggt tctgccgtcg ggaaatttca
      961 ggtcaaagaa tctcagtgca aaaacattgt cggtaccaaa gggccatgtc tttactgtta
     1021 ttctctcatc cctgaaggga tattcactga tttccgcata cattcttgac aggacccaga
     1081 aattattttc cttcatgagg tcgtctcttc cgtatcccag tctgattgcg tgttcagcgg
     1141 caatatcctg catatagtta aagagtgcac agatattcgc tttttcgtct acaaaggttt
     1201 catagacatg taccctgaac tctctttcaa aatgtggtat ggtcatgtta ttcatcttca
     1261 ttcaggaaaa cttccagttc atgtgtatat tgtgcccatc tgtccatttc ttcagcatgt
     1321 ctttccttaa gctcccggta cttttcatag aattcccggg ttgcttccgc cagatgtccg
     1381 ggtgagttca taagcgtatc aatgcgggat atttcctttt caaggttttc gatcatactt
     1441 tcagatttct caagcctgct tctcagtctg cggagatttc gttcatagtc ttttctttcc
     1501 aggtatttcc gtttgcctga ggaaatgttt ccgttttccc tgtcttcctt tctttgcagc
     1561 ggcgctgcct cctttctttc gatctccctc aggttttcaa ttttcctttt gcgcaggaaa
     1621 tcgtatatcc ccccgatatg ctccctgatt gcatggtttc tgaattcgta caccttgctt
     1681 actataccgt caaggaaatc cctgtcatgg gaaaccacaa ttagcgtgcc gtcgtaactg
     1741 atcagggcct gtttcagaat atccttggat cgcatgtcga ggtggttcgt aggctcatcc
     1801 agaacgagaa ggttatgtgg ttccagcagc agtctcacga gggcaagcct tgagcgctcc
     1861 ccgcccgaca gtactttcac tttcttatcc gcgtcatcac cccggaaaag gaaggcaccg
     1921 agcatatccc tgatctttgt ccggatttct cccctggcaa catcatcaac agtttcaaac
     1981 accgtttttt cctcattcag gagttcatcc aggttctgtg cgaaataccc ggttctgaca
     2041 ttatgtcccg ttttaagcag tccttcatga tccagttccc cgataatgat ttttgaaaaa
     2101 gttgttttcc cttccccgtt gcggcctaca aaagccacct tttcaccccg gtgtatcctg
     2161 aagtcgatcc tgttcagcac attgagcgtt ccgtagtttt tcgacagttc tcttgcgtca
     2221 accacaacgg tgcctgaccg gggtgccggc gggaatctca ggctgatttc actgaaatcc
     2281 tcttcatcga cctctatccg ttcaatcctg tcgagctgtt tgatccttga ctgtacctgt
     2341 atggcctttg tggccttgta tctgaacctt tcaatgaatt tttcggtatc atcaatcatt
     2401 ttctgctggt ttctgaaggc tgccagctgc tgttccctcc tttcctttct cagaaccgca
     2461 aattccgacc atgatacctt gtagtcatat attttcccca gtgagatttc tatggtccgt
     2521 gtggtgacat tgtcaagaaa ggccctgtcg tgtgatacca gtatcaccgc cccgcggtat
     2581 gatgcgagga atccttcgag ccactgtatc gattcaatgt cgatatgatt ggtcggttca
     2641 tccaggagca gcaatgacgg attctgcaga aggatctttg ccagttcaat cctcattctc
     2701 catccgccgc tcagctcccc cgtgggcctg tcaaaatccc tgtcctcaaa acccaggccc
     2761 ctgagtatct tttctgaatc ggcaaggtag ttgtctccac cggcatatct gaatttttct
     2821 tcaacgagat ggaggttttc gcaaagttta aggtattcgg gggattcata gtctttgcgg
     2881 gaggcgattt cagccgtgca ggattcaatc tcccgttcca ggccggttat ttcgggaaag
     2941 gctttaatga cctcgttaag cacggttgtt gtatcatcaa gtttcatctg ctggggcagg
     3001 tatccgatgg taacttccct cgacatgtca acctgtcctg aagtagggct ttgcaatccg
     3061 gctattatct tcag
//
LOCUS       DAOSPX010000364         3072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_28770_length_3072_cov_3.965529, whole genome shotgun sequence.
ACCESSION   DAOSPX010000364 DAOSPX010000000
VERSION     DAOSPX010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28770_length_3072_cov_3.965529"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PLZ75_13195"
     CDS             <1..1024
                     /locus_tag="PLZ75_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOS73394.1"
                     /translation="HITKKVADRLNEIFKKNRPEFEKKWDSLKLFINYGMITEEKFYE
                     KAAKFALLKNTDDKYFTFEEYEKLIKENQTDRHKTLIYLYATSKTDQYPYIEKARNKG
                     YDVLLLDGQLDVHLINHLETKFRDSRFVRVDSDVVEKLILKDEERESRLTDEQKDDLK
                     SVFNVNVKSGEMYNVVFEVLDENESPVLITQSEFMRRMKDMAQLGGGSMGFYGDLPDS
                     YNLVVNPNHPLIGKIYEDLRSENATVLDENAAERKKVKEEYDFLQNEHSKKKDEEISQ
                     PEKDDLEKLRKELEEIEDSRKKVLEAFGSSNKVVKQLIDLALLANNMLRGEDLSTFVR
                     RSVELL"
     gene            complement(1168..1689)
                     /locus_tag="PLZ75_13200"
     CDS             complement(1168..1689)
                     /locus_tag="PLZ75_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73395.1"
                     /translation="MEAVTDILKITLPALLVTITAWLVMRNMLRNERDMRRQELILQN
                     SRTVVPIKLQAYERIILFLERISMESLLVRVSTPNMNAAQLHSGLLTSVRSEFEHNLS
                     QQIYMSPQAWEVVRNARSGTIKLINSEFEKLPENASGMAFSKQLLTAVMDMDKEPTRV
                     AIDFIKAEAGRML"
     gene            1793..2986
                     /locus_tag="PLZ75_13205"
     CDS             1793..2986
                     /locus_tag="PLZ75_13205"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOS73396.1"
                     /translation="MEYLSDRIRTMATSATMAMARKSRELKARGLDIISLSLGEPDFN
                     TPAYIREAAKEAIDGNYSKYPPVSGYVDLRDAICKKFRDENGITYTRDQVVVSGGGKH
                     SIINVILSIVNPGDEVIILAPYWVSYYDQILIAGGRPVILETTIENDFKVSLEQLESA
                     ITDKTRLVIFNSPSNPTGMVYSRGEQEAIARVIQRHEGVFLMSDEVYEHILFEGEHVS
                     MASFDFIYDRVITVNAVSKAYAMTGWRIGYIGAPKWIADACDKLQGQFTSGVSSIAQR
                     AALAALSGKDGSIQMMRDAFLRRRDLICNQLSGIRGLRVRVPQGAFYVLPDISYYLGK
                     TDGTEIVRTADDLALWLLDRAGVATVGGDAFGAPGYIRISYANSDDLLTEAVRRIKEA
                     LEILK"
BASE COUNT          863 a          650 c          780 g          779 t
ORIGIN      
        1 ccatataacc aaaaaagtgg ctgacaggct gaatgagatt ttcaagaaaa accgccctga
       61 atttgagaag aaatgggaca gcctcaagct cttcatcaat tacgggatga taactgagga
      121 aaagttctat gagaaggcag ccaaatttgc attgttgaag aatactgacg acaagtactt
      181 tacctttgag gaatatgaaa agctcataaa ggaaaatcag accgacaggc acaaaacact
      241 gatttatctg tatgccacca gcaagacaga ccagtacccc tatatagaaa aggcaaggaa
      301 caagggttat gacgtcctgc ttcttgacgg acagctggat gtgcatctta ttaaccatct
      361 ggaaacaaaa ttcagggact caaggtttgt cagggttgat tcggatgttg ttgaaaagct
      421 catcctgaaa gatgaagaga gggaatcaag gctcaccgat gagcagaagg atgacctgaa
      481 aagcgtgttc aatgtgaacg tgaaatcagg tgaaatgtac aatgtagtct ttgaagtgct
      541 tgatgaaaat gaatcaccgg ttcttatcac ccagtcggag ttcatgcgca gaatgaagga
      601 tatggcccag ctgggcggag gaagcatggg cttttacggt gatctgcccg acagctataa
      661 cctggtggtt aatccgaacc atccgctgat agggaagatt tatgaggatc tgagatctga
      721 aaatgccact gttcttgatg aaaatgccgc tgaaaggaag aaggttaagg aggaatatga
      781 tttcctgcag aatgagcata gtaaaaagaa ggacgaagag ataagccagc cggaaaagga
      841 cgatcttgaa aaactgagga aggagctgga ggaaattgag gattcaagga agaaagtcct
      901 cgaagctttc ggcagcagca acaaggttgt caagcagctt attgaccttg ccctgctggc
      961 aaacaacatg ctgaggggag aagatctcag tacatttgtc aggcggagcg ttgaattgct
     1021 gtagcaaaat tgcctgctgt agctgaatgg ccatagctga attgcaatag ctgaattgct
     1081 gtagcgtaat agctgtagct gaattgcctt ctgcagctga attgaaccgg ttccggaggg
     1141 ttcagcgtta tgcctcagcc cgtaccttta caacatccgg ccggcctctg ccttgatgaa
     1201 gtcaattgcc accctagtgg gctccttgtc catatccatt acggcagtca gaagctgttt
     1261 gctgaaagcc atgccggaag cattctcagg cagtttttca aactcactgt taatcagctt
     1321 tatggtgcct gagcgggcat ttctgacgac ttcccatgcc tggggactca tgtatatctg
     1381 ctgtgagaga ttgtgttcaa actcgcttct gacgcttgtc agcaggccgg aatgaagctg
     1441 tgccgcattc atattcggtg tgctgactct caccagcagc gattccattg agattctttc
     1501 aaggaaaaga ataatccttt cgtaggcctg gagctttatg ggaacaacag tccggctgtt
     1561 ctggagaata agttcctgcc ttctcatgtc ccgctcattc cttagcatgt ttctcattac
     1621 gagccatgca gttattgtga ccagcaatgc aggcagggtt atcttaagta tgtctgtaac
     1681 tgcttccatg gtgaattatt ttgtcaataa tatgcaaact taacttattt tgtatttaat
     1741 ttttacttga tatatttgac cccgcccgta attttaacat atttaccctt atatggaata
     1801 tctatcagac agaatcagga ccatggcaac ttccgccaca atggccatgg cccgcaaaag
     1861 ccgggagctg aaggccaggg ggctggacat catcagcctg agtctcggag aacccgactt
     1921 caatacccct gcctatatca gggaagctgc aaaggaggca attgacggaa attattcaaa
     1981 atatccccct gtttcaggat atgttgatct cagggatgcc atctgcaaaa aattccggga
     2041 tgaaaacggg atcacctata ccagggatca ggtggttgta tcaggcggag gaaagcattc
     2101 cataataaat gtcattttat ccatagtaaa ccccggggat gaggttatca tacttgctcc
     2161 ctactgggtg agctattatg accagatact gattgccggc ggccggcccg taatccttga
     2221 aacaactatt gagaatgatt tcaaggtcag cctggagcag cttgaatctg caattactga
     2281 taaaacaagg cttgtaatat tcaactcgcc gtcaaacccg acgggaatgg tttattcgcg
     2341 cggggaacag gaggcgattg cccgggtgat tcaaaggcat gagggtgtgt tcctgatgtc
     2401 ggatgaagtt tatgaacata ttctctttga aggggaacat gtaagcatgg cttcatttga
     2461 ttttatttac gacagggtta taacggtaaa tgctgtttcc aaggcttatg caatgacagg
     2521 atggaggatt ggctatatcg gtgccccgaa atggattgcg gatgcctgtg acaaactcca
     2581 gggtcagttc acatccgggg tgagttccat tgcccagcgc gcggcacttg ccgccctttc
     2641 cggaaaggat ggctcaatac agatgatgag ggatgccttt ctccggcgcc gtgacctgat
     2701 ctgcaaccag ctttccggaa tcagggggct cagggtcagg gtgcctcagg gtgcttttta
     2761 tgttctgccc gatataagct attacctggg aaaaacagac ggcacggaaa ttgtgagaac
     2821 tgcagatgac ctggcactct ggctgcttga cagggcaggg gtggctaccg tgggaggcga
     2881 tgccttcgga gcaccgggct atatcaggat atcatacgct aattccgatg atctcctcac
     2941 ggaagcggtc agaaggataa aggaagcact tgaaattctg aagtgaaaac gacaataacc
     3001 ggatacaaca ataattgtta tctttctcct ataaatccat cccaaattat agcgcctttg
     3061 gggttgcgag tt
//
LOCUS       DAOSPX010000365         3066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_28853_length_3066_cov_4.227831, whole genome shotgun sequence.
ACCESSION   DAOSPX010000365 DAOSPX010000000
VERSION     DAOSPX010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28853_length_3066_cov_4.227831"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLZ75_13210"
     CDS             <1..851
                     /locus_tag="PLZ75_13210"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOS73397.1"
                     /translation="YAGIIPVKIIISEELKRRKNELSYFKSLSIDDSGSRIKQSFDRY
                     DIFIAAGGDGTVRSVASELIGTNKILGVLPMGSGNGFAKELGFRTNIRSLLKDIKKGE
                     KLQIDVIKINDMNCLNVAGIGLDSFVAHSFDRLRTRGFWSYAWVTMLNFIRLKPVHIE
                     IIPEGEEKISEDLFVLTIANTRQFGNNAYIAPDARPDDGIIDLVMIRPFPKFMFPVFI
                     YRLFRGTLKDSKYVKCLKTDRPLTIRTSETRFHIDGEPVEIPGDVSVKIISNSLSVLK
                     TRHNKL"
     gene            1026..2018
                     /locus_tag="PLZ75_13215"
     CDS             1026..2018
                     /locus_tag="PLZ75_13215"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOS73398.1"
                     /translation="MKRRDFVGKTMIAGGMMAPAAGLMSPGPQGRPVPPDEEIVIERA
                     VAGRPHEGKVLAVIQPHCDDIAYFAAGTVAKLIHEGYTAYLIRTTNDDAAGSGSSYGE
                     RVLNNEKSNEAFARVMGFKKVYDLGYRNHRMDEYNIQEIKGRLIFLFRLLKVDTIVSF
                     DPWDHYEENPDHYVTARAVEAARWMAGMGTDYPEQLEVVDPHPVIERYYYARGPQVFN
                     RIVDISGFIDRKTEANMAVIAQGSGGHAGSNLRKRLAAEGKKLPLLGNDDRTADFNYI
                     KNFMLDMYSEQMRGIPSDRELGKYYGLEWAERFRYFGPQKSGLNDYISKNAVKL"
     gene            complement(2117..2689)
                     /locus_tag="PLZ75_13220"
     CDS             complement(2117..2689)
                     /locus_tag="PLZ75_13220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS73399.1"
                     /translation="MKKMSASWKTRLVIVMVIISQVAVACRKSGQKDDETPPVLSVTP
                     VDITTVRHIIPFGADLRPGQKNPAFEYYVNGSSVEVRSVCAGYVEDIRLNDNFPDYEV
                     WIKTSSASVFKLIYDHVVDLNVKKGDKVDAGTVLGKVGNGDRTELQINKSDRDKGELS
                     YCPFDFATDDFISRHKQFTDTWCLTGTVRP"
     gene            complement(2686..2931)
                     /locus_tag="PLZ75_13225"
     CDS             complement(2686..2931)
                     /locus_tag="PLZ75_13225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOS73400.1"
                     /translation="MPGSSSGPMSNPLIIVDGIIKDISINSIDPEIIKSVNVLKGDFA
                     FRRYGVEGKDGVIEITTKTGSVTPGNDSGQKGKRHRS"
BASE COUNT          877 a          673 c          738 g          778 t
ORIGIN      
        1 cttatgcggg aataatccct gtcaaaatca taatatccga agagcttaaa agaagaaaga
       61 atgaattatc gtatttcaaa tcactgagta ttgatgattc cggttctaga ataaagcaat
      121 cattcgacag gtacgacata tttattgcag ccgggggcga cggaacggtc agaagcgtgg
      181 catcggaact gatcggaaca aataagattc tcggtgtact tcccatggga tcgggaaacg
      241 gttttgccaa ggagctggga ttcaggacaa atatccgttc tctcctgaag gatatcaaga
      301 aaggtgaaaa gctgcagatc gatgtaataa agataaatga catgaattgc ctgaacgttg
      361 caggcatagg ccttgacagc ttcgtggccc attccttcga caggctcaga acaagagggt
      421 tctggtcata tgcatgggtt accatgctta atttcatcag gttgaaacca gttcatatag
      481 agattattcc ggaaggagaa gaaaaaattt ccgaagatct ctttgttctg accatcgcca
      541 atacaagaca gttcggcaat aatgcatata tagctcccga tgccagaccg gatgacggta
      601 tcattgatct ggtgatgatc cggccatttc ctaaatttat gtttccagta ttcatttaca
      661 gacttttcag gggaaccctg aaagattcga aatatgttaa atgcctgaaa accgacaggc
      721 ccctgaccat cagaacgagt gaaacaagat ttcatatcga cggcgagccg gtcgaaatcc
      781 cgggagacgt atcggtaaag attatcagca attccctgtc tgtgcttaag accaggcata
      841 ataaactatg agtctgaact gcaggcatgt tttctccgct tatttaccct tcacctgaat
      901 aaggcggtac gccggtgaat aaattgaaca aaacttagta ataataaaaa taaatcattc
      961 atatatagaa cataagcaca ataaacaatt accaatacat aatgaatatt gaatcaggaa
     1021 ataaaatgaa acgaagggat tttgtaggaa aaacaatgat tgcaggtgga atgatggctc
     1081 cggctgccgg tttgatgtct cccgggccac agggaagacc tgttccgcct gatgaggaaa
     1141 tagtcataga gagagctgtc gcaggcaggc ctcatgaagg aaaagtgctg gcggtaatac
     1201 agcctcattg tgatgatata gcctattttg cggctggcac cgtagccaag ctcatccatg
     1261 agggctatac tgcatacctg atcaggacga ccaatgatga tgccgcggga agcggcagtt
     1321 catacgggga gagagtcctg aacaatgaaa aatccaatga ggcatttgca agggtgatgg
     1381 gtttcaaaaa ggtctatgac ctcgggtacc gtaatcacag gatggatgaa tacaatatcc
     1441 aggaaataaa aggaaggctc atattcctgt tcaggcttct gaaagttgat actattgtca
     1501 gctttgatcc ctgggaccat tatgaagaga accccgacca ctacgttact gcccgtgctg
     1561 ttgaggcggc acgctggatg gcaggaatgg gtaccgacta tcccgaacag cttgaagtgg
     1621 ttgaccctca tccggtgatc gaaaggtatt attacgctag aggaccccag gtcttcaacc
     1681 gtatcgttga catttccggt ttcatcgaca gaaaaactga agccaatatg gctgttatag
     1741 cccagggcag cggcggacac gccggatcaa acctcaggaa aagactggca gcggaaggaa
     1801 agaaacttcc gcttctcggc aatgacgacc gtaccgccga tttcaactac atcaagaatt
     1861 tcatgctgga catgtattct gaacagatga gaggcatccc gtccgacagg gaactcggaa
     1921 aatattacgg acttgaatgg gctgaacggt tcaggtattt cggtcctcaa aaatccggac
     1981 tgaatgatta catcagtaaa aatgcggtga aattatagtt atccatgcaa gtccccgatt
     2041 aagtactgct tcatgtcgta acggcctgtt cttctatcag tttcgaaggc aacaggcgga
     2101 tccggaatga taaatattat ggtctgacag tgccggtaag gcaccatgtg tcggtgaatt
     2161 gcttatgcct gctgataaaa tcatcagtgg cgaaatcgaa ggggcagtat gataattctc
     2221 ccttgtccct gtcactcttg tttatctgaa gctcggtccg gtcaccattt ccaactttgc
     2281 cgagaacagt accggcatca actttatcgc cctttttcac attcagatca acgacatgat
     2341 cgtaaatcag tttgaaaaca gaagctgatg atgtcttaat ccatacctcg taatcgggaa
     2401 agttgtcatt cagcctgatg tcttccacat agccggcaca aacagatctc acctcaacgg
     2461 atgaaccgtt tacataatac tcaaaggcag gatttttctg tccaggcctg agatcggcac
     2521 caaatggtat tatatgcctg actgttgtga tatcaaccgg tgtcactgac aaaacaggtg
     2581 gcgtttcatc atccttctgt ccgcttttcc tgcaggcaac agccacctgg gaaatgataa
     2641 ccatcacaat aaccagtctg gttttccagg atgctgacat ttttttcatg acctgtgcct
     2701 tttccccttt tgtccggaat cattaccggg tgtgacagat ccggttttcg tggttatttc
     2761 aatcactccg tctttccctt ccactccata acgcctgaaa gcaaaatcac ccttcaaaac
     2821 attgacggat ttgataattt caggatctat tgaatttata ctgatatctt taatgatacc
     2881 gtcaactatt ataagaggat tgctcatcgg tccggaagag ctgccgggta ttggtatacc
     2941 ggtattcttt aatgacgata tggcagcatc attgtccctg cccgcgggcg gtggcggcgg
     3001 cggtggcgga ggtggcggcg gcggcggcgg aggtggcggc ggcggcggcg gaggtggcgg
     3061 cggcgg
//
LOCUS       DAOSPX010000366         3060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_28934_length_3060_cov_4.176705, whole genome shotgun sequence.
ACCESSION   DAOSPX010000366 DAOSPX010000000
VERSION     DAOSPX010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28934_length_3060_cov_4.176705"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /locus_tag="PLZ75_13230"
     CDS             <1..2302
                     /locus_tag="PLZ75_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS73401.1"
                     /translation="AVSNHLRTKYGYETGSFSSFPASVSNLKYLVRLDWNINRDNKLS
                     VRFNHTKNIEWFGPNGNSTDGAYRDQGKNRIGEYSMSYANSMYSQDNLVNSGSVELNS
                     RFSDRISNQLLITYSNIEDIRGSNSAPFPFIDIMSGDIADGAAALDPYISAGYELFTW
                     NNGVKNKIINATDNFTYYLDLHKITAGVSFEYQMADNNYMRSGTGYYRYASLSDFLTG
                     AAPVDFALTYGTNGILVPSNAVKFNQIGAYIQDEWSPTYNFKLTYGLRADNITFIQDI
                     MTNNAILELDFGGRSINTGKWPTPKVNLSPRVGFTWDIAGDKSVVLRGGTGLFTGRLP
                     LVFFTNMPSNAGMNQMLMKIQTRFNADGSVRSSDPLLDRLAGDIITDVNDMISRMDFQ
                     TTVTPQDGSVPSSIAGVDPSFRMPQVWKTSAAFDYRAPVDFPLSFTAEGIFTKNINAV
                     RLENYAVKNPQAGWDKFSGPDDRYIFPPGYLYFSSVKDACVLVNTNKGYGYTLNLTVN
                     AEPVSNLDLMFAYTKTEMREVSGMPGSNANSAWVGLITVNGPNNSTVQRSQYVVPDKV
                     IASASYRVPYLNDLMASHFSIFFTGLTPYGNTYRYSNDMNGDGVNNDLIYIPKEKGEI
                     SFVTPEDENAFFAFVEQDKYLRKHKGEYAEAYSARSPWVNRVDLRFVQDFTVKAGQTK
                     NTLQLSVDLLNAGNLLNSKWGVYSNNKISNNGAVLKYEGKDVANNVPSFSMIQDKGKY
                     LDKSYSHFTDLSQVWRLQVGLRYIFN"
     gene            2734..>3060
                     /locus_tag="PLZ75_13235"
     CDS             2734..>3060
                     /locus_tag="PLZ75_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOS73402.1"
                     /translation="MKKEEFSHDEAVKASIGYFRGDELAAKVWANKYALKDSFGKLYE
                     KTPDDMHRRLAREIHRIESKYRNPLPEELIYSVLKDFKYIIPQGGPMTGIGNDFQIAS
                     LSNCFVI"
BASE COUNT          895 a          691 c          703 g          771 t
ORIGIN      
        1 ggcagtgtca aatcatctca gaacaaaata cggttatgaa accggttcat tcagcagttt
       61 tcctgccagt gtgtcaaacc tgaaatacct ggtacgcctc gactggaaca taaaccgtga
      121 caacaaactg agcgtgcgtt tcaaccatac aaagaacatt gaatggttcg ggccaaacgg
      181 caactcaact gacggagcct accgtgacca ggggaaaaac cgtataggtg aatactcaat
      241 gtcctatgcc aattccatgt attcccagga taaccttgtc aattcaggat cagttgaact
      301 taacagccgc ttctccgaca ggatatccaa tcagctgctg ataacctatt cgaatattga
      361 agatatccgt ggttcaaact cagccccctt cccctttatt gacataatga gcggggatat
      421 tgcagacggg gcggcagcgc tggatccgta catctcagca ggctatgagc tgttcacatg
      481 gaataacggg gtgaaaaaca agataatcaa tgcaaccgac aatttcacct attatcttga
      541 ccttcacaaa ataactgcag gcgtaagttt cgagtaccag atggcagaca ataactatat
      601 gcgcagcgga acaggatatt accgctatgc cagcctgagt gattttctta ccggagccgc
      661 tcctgtggac tttgccctta cctatggcac caacggtata cttgtgcctt ccaatgctgt
      721 taagttcaat cagataggtg catatatcca ggatgaatgg tctccgacct ataatttcaa
      781 actgacctat gggctgcgtg ccgataatat taccttcatt caggatatta tgaccaataa
      841 tgctatactg gagcttgatt tcggcggccg cagcatcaat accggcaagt ggcccacgcc
      901 aaaggtcaat ctgtcacccc gtgtcggttt tacctgggat atagcagggg acaaatcggt
      961 ggttctcagg ggaggaaccg gcctgttcac cggcagactg cctctggtat tcttcacgaa
     1021 catgccctcc aatgccggca tgaaccagat gctgatgaag attcagacca gattcaatgc
     1081 cgatggttct gtcaggtcca gtgacccgct gctggacaga cttgccggcg atatcataac
     1141 agacgtgaat gatatgatta gcaggatgga ttttcagact actgttactc ctcaggacgg
     1201 aagcgttccc agttctatag caggagtcga tccctcattc agaatgcctc aggtctggaa
     1261 aacatcagct gcctttgatt acagggcacc ggttgatttt cccttatcct ttaccgctga
     1321 aggtattttt actaaaaaca taaatgctgt aaggctggaa aactatgcag tgaagaaccc
     1381 gcaggcaggc tgggacaaat tttccggtcc ggatgaccgt tatatttttc ctcccggata
     1441 cctgtacttc tccagtgtaa aggatgcatg cgtgctggtc aataccaaca agggctacgg
     1501 gtatacgctg aacctgaccg tcaatgcgga accggtaagt aatctcgacc tgatgtttgc
     1561 atatacaaag accgaaatga gggaagtgtc cggaatgccc ggcagcaatg ccaattcagc
     1621 ctgggtgggg cttatcactg tcaacggtcc caataattcc acggtacagc gctcgcagta
     1681 cgtggttccc gacaaggtga ttgcctcggc atcataccgg gttccatacc tgaatgacct
     1741 gatggcatca catttcagca tattctttac cggtctcacg ccctatggca atacataccg
     1801 ctacagcaac gacatgaatg gtgatggcgt aaacaatgac ctgatttaca ttccgaagga
     1861 aaagggcgaa atatcatttg tcacccccga agatgaaaat gcattttttg catttgttga
     1921 acaggataaa tacctgagaa agcataaggg agaatacgca gaggcctact ccgcccgctc
     1981 accctgggtc aacagggttg acctccgttt tgtgcaggat ttcaccgtta aggcaggaca
     2041 gaccaaaaat acccttcagc tgagtgttga cctgctgaat gccggaaatc tgctgaactc
     2101 aaaatggggt gtatacagta ataataaaat aagcaacaat ggagccgtcc tgaaatatga
     2161 gggaaaggat gtagcaaaca atgttccgag tttctcaatg attcaagata agggaaaata
     2221 cctggacaaa agctattccc attttaccga cctcagccag gtctggagac tgcaggtggg
     2281 actgcgctat attttcaact aaccggaaga ctgaactgat acagataaaa agcgcctgga
     2341 aacggcgctt ttttctttcc ggtaatcttc gggattacag tgcctttatg tttttttact
     2401 gtctccggtg ggaaggacca catttcataa catacagata atgagaaata tacaaaaagt
     2461 taataactct ctcttcaaat gttaataaac ttaaattttt tgtcttgctt tcagattttc
     2521 acttggctat tttagcaaat acccgtttta aatgtcgacg ggcgtttaat ctcaattaaa
     2581 tcagagaata acagaattta caccttgaat atggaagaac tatttgtcca gggatcaact
     2641 attgtttctg aaaacgtgaa tgaagaaaaa acattacccg gagctcagga tctgaataag
     2701 gaagagaagg gtacagaatc tgtaaaaagg aatatgaaaa aggaggaatt ttctcatgac
     2761 gaagccgtta aggcaagtat cgggtacttc aggggcgatg aactggcagc aaaggtctgg
     2821 gcaaataagt atgcgctgaa agattctttc gggaaactct atgagaagac tcccgatgac
     2881 atgcatcgca ggctggcacg ggagattcac cggatagaaa gcaaatacag gaatcctctt
     2941 ccggaggagc ttatttacag tgtactgaag gatttcaaat atatcattcc gcagggcggg
     3001 ccgatgacag gtattgggaa tgacttccag atagcaagtc tgtcaaactg ctttgttatc
//
LOCUS       DAOSPX010000367         3039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_29212_length_3039_cov_2.838807, whole genome shotgun sequence.
ACCESSION   DAOSPX010000367 DAOSPX010000000
VERSION     DAOSPX010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29212_length_3039_cov_2.838807"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PLZ75_13240"
     CDS             <1..1176
                     /locus_tag="PLZ75_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOS73403.1"
                     /translation="LFHDKLRPGILYTSPVSGTVRSIERGDRRKMLEVVVEKSGDDYV
                     DFGKADPESLSAEKIKERLLASGLWPAIRQRPYQVVADPADRPRAVFISGFDTAPLAP
                     DNYFIIENSESSLFLTGIRAVSRLTEGKVHLVLDGKRKAPALLEECPGVEVSYFSGPH
                     PAGNVGIHIHHLDPVNKGEVVWFVNLQDVISIGRLFTGGRYHPERIVALTGSEVIKPQ
                     YYRMLSGSSVENIIKDNINPGKARYISGNVLTGMKISEKGFLGYYDSQITVIPEGDYF
                     ELLGWAAPGLRKYSFHRAFLSNLLPSRSYNIDTNLHGGERAFVVTGKYEKVLPMDIYP
                     MQLFKAILAEDIELMENLGIYEIAEEDVALCEFICPSKIEIQSIVRKGLELMIKEMS"
     gene            1196..2359
                     /locus_tag="PLZ75_13245"
     CDS             1196..2359
                     /locus_tag="PLZ75_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOS73404.1"
                     /translation="MNSLRKQLDKLRPHFSEGGRFQKLHSVFEGFETFLFVPDKVTFK
                     GSHIRDNMDLKRTMTVVIIALVPAILFGCYNTGLQHFRSTGTDASVLQLFWYGFLRFL
                     PLIIVSYITGLGIEFAAAQIRGHEINEGFLVTGILIPLIMPIDVPLWMLALATAFSVI
                     LGKEVFGGTGMNIFNPALLARAFIFFSYPAWMTGDKVWTKGLVSGANVIDGYSGATPL
                     GLCAQGHTADLPSFMEMFIGTIPGSVGETSVPAILIGALILIVTGIGSWKIMLSMFGG
                     GLTMALLLNALGTNEFMQLPFYYHFVMGGFAFGAVFMATDPVTASQTEKGKWIYGFMA
                     GILSILFRVLNPAYPEGVMLAILLMNLFAPLIDYFVIQSNIKRRIRRLQAVNQ"
     gene            2370..>3039
                     /gene="nqrC"
                     /locus_tag="PLZ75_13250"
     CDS             2370..>3039
                     /gene="nqrC"
                     /locus_tag="PLZ75_13250"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOS73405.1"
                     /translation="MRQFSNRYIFVFSTVMVVIVAVLLSLAATLLQPAQERNQEIEKK
                     KSMLESIRIPASRQNAVELYDKYIRESFVINTRGEVLEGIDAFNVALRNEQRKPAEQQ
                     SLPVFRAVTDEGEQVIILPLEGKGLWGPINGYISLRRDMNTVYGVTFDHKGETPGLGA
                     EINTPGFENMFRDKKLYEDQTFVSVKVVKGGARKDDVHGVDAVSGGTITSRGLENMLF
                     DCLEK"
BASE COUNT          804 a          645 c          793 g          797 t
ORIGIN      
        1 ctttttcatg acaagctcag gcccgggata ctttacacat ctcccgtaag cggtactgta
       61 agatccattg aaaggggcga ccgccggaag atgctggaag tggttgtgga gaagagcggt
      121 gatgattatg ttgatttcgg taaggcagat ccggaatccc tttctgcaga gaagataaag
      181 gagagacttc ttgcttcggg cctctggccc gcaatacggc agaggcccta tcaggtggtg
      241 gcagatccgg cagacaggcc cagggcagtt tttatttcag gatttgatac ggcacctctg
      301 gctccggata actatttcat aattgaaaat tcggaaagtt cactgttcct cacaggtatc
      361 agggcggttt cccgactgac agaggggaag gttcaccttg tgcttgacgg aaagagaaaa
      421 gccccggccc tgcttgagga atgccccgga gtggaagtgt catatttttc aggaccccat
      481 cctgccggga acgtgggaat tcatattcat catctcgatc cggtaaataa gggcgaagtg
      541 gtttggttcg tcaatcttca ggatgtgatt tccataggca ggctatttac cggaggcagg
      601 tatcatccgg aaagaattgt ggcacttacc ggttcggaag ttattaagcc tcaatactac
      661 aggatgctat ccggcagttc ggtggaaaat atcataaagg acaatataaa tcccgggaaa
      721 gccaggtata taagcggaaa tgtccttacc ggcatgaaaa tatctgaaaa aggtttcctg
      781 ggttattatg attcacagat aacggtgatt cccgagggcg attattttga actgcttggc
      841 tgggctgcgc ccggattaag gaaatacagc tttcaccgcg ccttcctgtc aaacctgctt
      901 ccttcaagat catacaatat tgatacaaat cttcacggag gggaaagggc atttgtagtt
      961 accgggaaat atgaaaaggt gctgccgatg gatatttacc ccatgcagct gttcaaggca
     1021 atccttgcgg aagatattga actgatggag aatctcggta tatatgaaat agccgaggaa
     1081 gatgttgccc tctgtgaatt tatctgccct tcaaaaatag agatccagtc tatcgtcagg
     1141 aaagggctgg aactcatgat aaaggaaatg agttaataaa actgttaatt ataaaatgaa
     1201 ttcccttaga aaacagctgg ataagctcag gcctcacttc tctgaaggag gcaggtttca
     1261 gaagttgcat tcggtatttg agggctttga aacctttctg tttgttccgg acaaggttac
     1321 ttttaaagga tcccatatcc gcgacaacat ggacctgaaa cggacgatga cggtggtcat
     1381 catcgccctg gtccctgcta ttctttttgg ctgttacaac accggccttc agcatttcag
     1441 atctacaggc acggatgctt ccgttctgca actgttctgg tacggcttcc tcaggtttct
     1501 gcccctgatc atagtaagct acataacagg acttggcatt gaattcgcag cggcacagat
     1561 cagaggtcat gagatcaatg aaggattcct tgtaacgggt attctcatac cgctgataat
     1621 gcctattgat gttccgctct ggatgcttgc ccttgcaaca gctttctcag tcattctggg
     1681 aaaggaagtt ttcggcggca ccggaatgaa tatatttaat cccgcccttc ttgcacgggc
     1741 attcattttc ttcagttatc cggcatggat gacaggcgac aaggtctgga caaaaggact
     1801 ggtgtccgga gccaatgtga ttgacggata ttccggggca accccgctgg ggctgtgtgc
     1861 acagggacat actgccgacc tgccttcctt tatggaaatg tttatcggaa caattccggg
     1921 ttcagtgggt gaaacctctg ttcccgcaat attgatagga gcactgatac tgatagttac
     1981 aggtatcgga agctggaaga tcatgctcag catgttcgga ggaggactta ccatggccct
     2041 tcttcttaat gcccttggaa ccaatgagtt tatgcagctg cctttttact atcattttgt
     2101 aatgggaggc tttgccttcg gggcagtttt tatggctacc gatccggtaa cagcatcaca
     2161 gacggaaaaa gggaagtgga tctacggctt tatggcagga attctttcaa tacttttcag
     2221 agtgcttaac cctgcctatc ctgaaggggt aatgcttgca atcctgctga tgaacctttt
     2281 tgccccgctg atcgattatt tcgtaataca atccaatata aagagaagaa tcaggcgtct
     2341 tcaggcggta aatcaataat ccaaaaaata tgaggcaatt cagtaacagg tatatttttg
     2401 ttttctcaac tgtgatggtt gtgattgtcg ctgtattact ttcgcttgca gctacactgc
     2461 ttcagcctgc ccaggaaagg aatcaggaaa tcgagaaaaa gaagagcatg cttgaatcga
     2521 tacgcatacc ggcaagccgc cagaatgctg ttgaattgta cgacaagtat atacgggaaa
     2581 gttttgtaat caataccagg ggggaagtcc tggagggtat tgatgctttc aacgtggccc
     2641 tcaggaatga acagaggaaa cctgccgaac agcagtccct gcccgttttc agggccgtga
     2701 cggacgaagg cgagcaggta ataattcttc ccctggaggg caaggggctg tgggggccca
     2761 ttaatggcta tatttctctg agacgggata tgaatacggt atacggggtg acatttgatc
     2821 acaagggtga aacgcccgga ctgggagctg aaataaacac tccgggtttt gaaaacatgt
     2881 tccgcgacaa gaagctctat gaagatcaga cattcgtatc ggtgaaggtg gtcaagggag
     2941 gagcccggaa agatgatgtt cacggtgtgg atgcagtctc gggggggacg ataaccagca
     3001 gggggcttga aaatatgctt ttcgactgtc tggaaaagt
//
LOCUS       DAOSPX010000368         3024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_29409_length_3024_cov_3.771977, whole genome shotgun sequence.
ACCESSION   DAOSPX010000368 DAOSPX010000000
VERSION     DAOSPX010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29409_length_3024_cov_3.771977"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..593)
                     /locus_tag="PLZ75_13255"
     CDS             complement(102..593)
                     /locus_tag="PLZ75_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73406.1"
                     /translation="MLKSLILTLSLILHPVHVTITSIEHIPATDSIKVFVRMYYDDFL
                     LDYCMFNEDADPDELSLTELLPDDHMNQYLAERVVLTVNNKRLYGRIINQTLAIGVDE
                     VEVSINLVYHTERQPEIFSIRNEIMTGLYEDQANMTIVRMSGLEEGLKLTPGKSEQTF
                     ILK"
     gene            complement(640..2088)
                     /gene="pyk"
                     /locus_tag="PLZ75_13260"
     CDS             complement(640..2088)
                     /gene="pyk"
                     /locus_tag="PLZ75_13260"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOS73407.1"
                     /translation="MLNRKRTKIVATISDKNCEPDFIRNLYEAGMDVVRINSAHLEIE
                     GALRIIRNVRKVSDKIAILIDTKGPEIRTTDCDEPVNIQTGTVIRMAGDSGKKTTPEV
                     IHVNLRSIADEVPVGSRVMIDDGEIVFRVIGKDRDRDMLILKSENMGLIGSRKSVNVP
                     GVKIDLPSLTDKDRIFIQMAAANDIEFIAHSFVRRKQDVLDVQAEIDKYNSGIKIIAK
                     IENQEGVENINDILDVVYGIMVARGDLAIEIPYEKIPTLQKMIISRCIYCRKPVIVAT
                     QMLHSMITNPRPTRAEVSDIASAVYSQTDALMLSGETAAGNYPVEAVRTMTKVALEVE
                     RNKEKLLETPFINVTGEISSYLAKVAVETSLRIGARGIIADTSGGRTIRDMASYRGFN
                     LILAQCYTTRAMRELALSYGVYTSYQEPRDSIDQFIHIALSNLISEYDFRGDDKVVVL
                     AGNFSGGNGFSFIEVGTVEYLNKRIGFLQDMN"
     gene            complement(2092..2526)
                     /gene="aroQ"
                     /locus_tag="PLZ75_13265"
     CDS             complement(2092..2526)
                     /gene="aroQ"
                     /locus_tag="PLZ75_13265"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOS73408.1"
                     /translation="MKISIINGPNLNLLGRREPEKYGSVTFEDYLEQLRKRYPGIEFS
                     YFQSNIEGKIIDEIQEKGFSTDGIIINAGGYTHSSVAIADAIASVTAPAIEVHITNIG
                     IREVFRHTSLVARSCRGSVIGLGLDGYRLGVEALIEIKANRQ"
     gene            2676..>3024
                     /locus_tag="PLZ75_13270"
     CDS             2676..>3024
                     /locus_tag="PLZ75_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOS73409.1"
                     /translation="MSLNWKQAVRDFATYLRLEKSLSENSISAYTGDVLKLMEYFDLH
                     NPGRMPAEVTYDDLRDFLAWFGSGEHSARSQSRTISGIRSFFSFLLTEEEISENPAAL
                     IESPKTGLKLPEVL"
BASE COUNT          779 a          758 c          604 g          883 t
ORIGIN      
        1 tctcctgccc tgttgtgggt ggccgagaga atcaggaaag tcagtaaaaa aaacaaagtc
       61 gcggataatc ttttcataat ctgaattctt ctagctccca attatttcaa aataaaagtc
      121 tgttcgcttt ttccgggtgt cagcttcagc ccttcttcca gtccgctcat ccggactatg
      181 gtcatattgg cctgatcctc atagagtccg gtcattattt cattcctgat gctgaaaatt
      241 tccggctgcc tctcagtatg ataaaccagg tttatgctta cctccacctc atcaacccca
      301 atggccagtg tctgatttat tatcctgccg taaagccgtt tattgtttac cgttaataca
      361 actctctctg caaggtattg attcatgtgg tcatccggaa gaagctccgt aagtgataat
      421 tcgtcaggat cggcatcttc gttaaacatg cagtagtcaa gcaggaagtc atcatagtac
      481 attcttacaa aaacctttat cgaatccgta gccggtatgt gttcaatact tgttattgta
      541 acatgaaccg gatgaaggat cagtgagagg gtcaaaatca gacttttcag cataatgcct
      601 tatcttaggg ccattaaatt ccatcaaaaa ccagaccaat cagttcatat cctgcagaaa
      661 tcctatacgc ttgttcaggt attccacggt accgacttca atgaaggaga agccgttgcc
      721 gcctgaaaag tttccggcaa gaacgaccac cttgtcatca cctctgaaat catactcact
      781 gatcagattg gaaagtgcaa tgtggatgaa ctgatcaatt gagtccctgg gttcctgata
      841 actggtataa accccgtacg agagggcaag ttccctcata gcccttgtgg tatagcactg
      901 ggcaagaatg aggttgaagc ccctgtagga agccatgtcc cttatggtcc tgcctccgct
      961 ggtatcggca attatacccc ttgcacctat gcggagtgat gtctccaccg ccacctttgc
     1021 cagataggag gaaatctcac cggtaacgtt aataaaggga gtttcaagaa gtttctcctt
     1081 gttcctttcc acctccaggg ctactttggt catcgtcctg acagcctcca cgggataatt
     1141 cccggctgca gtctctccgc tgagcatcag ggcatcggtc tgggaataca ctgcactggc
     1201 aatatcgctc acctcagccc tcgtgggcct gggattggtg atcatggaat gaagcatctg
     1261 ggtggctaca ataacgggct tcctgcagta aatgcatctc gaaattatca ttttctgcag
     1321 ggtgggtatc ttttcataag gaatctcaat tgccaggtct ccccttgcca ccataatgcc
     1381 ataaaccaca tcaaggatat cattgatatt ctcaaccccc tcctgattct caatttttgc
     1441 aataatcttg attccgctgt tgtatttatc tatttcagcc tgaacatcaa gcacatcctg
     1501 cttccttctc acaaaggagt gtgcaatgaa ctcaatgtca ttggcagcag ccatctgaat
     1561 aaatatcctg tccttgtcag tcagggaagg aaggtcaatt ttcactcccg gaacatttac
     1621 acttttcctt gatccgatca gacccatgtt ctctgactta aggataagca tatccctgtc
     1681 cctgtccttt cctataaccc tgaatacgat ttccccgtca tctatcatca ctctcgaacc
     1741 gacaggaact tcatcggcaa tgcttctcag gtttacatga atgacttccg gtgtcgtctt
     1801 cttcccggaa tctcctgcca tccttatcac agttcccgtc tgtatgttta ccggttcatc
     1861 acagtctgtt gtccgtattt caggaccctt ggtatcaata agaattgcaa ttttatccga
     1921 gaccttcctg acatttctta taatacgcaa agcaccttca atctcgagat gggcggagtt
     1981 tatccttacc acatccattc ccgcttcata aagattcctt ataaaatcag gctcgcaatt
     2041 cttgtcggaa atggttgcaa ctatctttgt tctcttgcgg ttcaacattt tctactgcct
     2101 gtttgcctta atttcgataa gcgcctccac tcccagcctg tagccgtcaa gacccaggcc
     2161 gatgacagaa cctctgcagc tgcgggcaac aagactggta tgcctgaaaa cctcacgtat
     2221 cccgatattg gtaatatgaa cctctattgc cggtgcagtc acggatgcga tggcatcggc
     2281 aatcgcaacc gaggaatgtg tgtatccccc tgcattgata ataatcccgt ctgttgaaaa
     2341 tcccttttcc tgaatctcat ctattatctt tccttcaatg ttcgactgaa agtagctgaa
     2401 ttcaataccg ggataccttt tcctgagttg ttccagataa tcttcaaatg tgactgatcc
     2461 gtatttttcc ggctccctcc ttcccagaag attgagattg gggccattta tgatgctgat
     2521 tttcattaca aaaataaaat tctttcaaaa ggcaaaaata gacaaaaaaa caatggctgc
     2581 cacataatta taagcctgcg gtttttatta actttgaggc ggtaaagacg ataccattca
     2641 gtattcaaag tgttttgccc cattgattta ttcacatgag tctaaactgg aaacaggctg
     2701 tcagggattt cgcgacttat ctccgtcttg aaaaatccct ttcggagaac agcatatcgg
     2761 cctatacagg tgacgtgctt aagctcatgg aatattttga ccttcacaat cccgggcgta
     2821 tgccggctga ggtgacatac gatgatctcc gggatttcct ggcctggttc ggaagcgggg
     2881 aacacagtgc aagaagccag tcaaggacca tttccggaat cagatcgttt ttcagttttc
     2941 tgctcacaga ggaagaaatt agtgaaaatc cggctgccct tattgaatcc cccaagaccg
     3001 gattgaaact gcccgaggtt ttat
//
LOCUS       DAOSPX010000369         2996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_29746_length_2996_cov_4.114247, whole genome shotgun sequence.
ACCESSION   DAOSPX010000369 DAOSPX010000000
VERSION     DAOSPX010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29746_length_2996_cov_4.114247"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="PLZ75_13275"
     CDS             complement(<1..1608)
                     /locus_tag="PLZ75_13275"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOS73410.1"
                     /translation="MYFKTGLLVLLFYLSGTYLFAQSSALLLEENFEYVFGTDLAGQG
                     DWVQGKAETNRVTVSEQGLSYTGYPGSGVGLAAHFSPLSDRVQKNFTGTLTGSYYYSF
                     LINVSSAGSGEFFAGFFSQNAFRGRIYLKAEGEGFQFGLVKTTTGTVTYTSGTPYQFG
                     STYLVVVQYDFVSGSANDQVSLFIDPDLEASTPGLPVVGPLSDAGNDVSANVFAMQAR
                     NNSGVFTIDAVRIATSWDAITGNDMENHYLELPRFISSDMVLQRDVPLKLHGWAAQGD
                     TVLVELVRSDSVYTDLVEVNADNKWEVELPAQAVSTEPCQLKFSIKGYPETLQELENI
                     LIGDVWFAGGQSNMEKKVNHLLEADTYISEADNYSQIRSFRAAYNLQNEPSDRVSGSS
                     SPWFVCNSSKVADNVSAVAYVFAREIHEKTGIPIGIMQAYRGGTEIETWISPWKLAEP
                     EYCIIQGRNAFLTASSYNNSHSVNFNGQINPLKGFPIKGFIWYQGESNTKRPLEYRYM
                     MKMLIDDWRSLWNQGDLPFYYVQMFNTA"
     gene            complement(1748..2821)
                     /locus_tag="PLZ75_13280"
     CDS             complement(1748..2821)
                     /locus_tag="PLZ75_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS73411.1"
                     /translation="MKTNLRNKTMFALLMAFGIITGVVAAGSVTETTVFSDNFNRVDI
                     SPGGSPEVTYTTTVTGTAVPIIQDGDLLRLPNVSGENARSQVTAGLAGYSSPFNSKLN
                     NIEADSLVWRFNVRQNYNGRLTGIDDASSRGIGVVLVASSSDLSVANGYAIINGGASP
                     IDYRLVKFSNGLTNGDNITILQHGQTLTDNRVYMSIKLVYLPSSNTWKLYDRNDGPAS
                     GGSFSDPLTDTGYVLAGSVVDNTHTSSDMSHFGFTHKYSGTTSFNFWVDNFSVKTYET
                     DMGVGINVNKNQSSVNWNYIQGGIQITAPAALLTLYDLTGSVVKAVQVKNQADIHIVN
                     PGIYVMKVQSSNGETSIEKILVK"
BASE COUNT          917 a          679 c          489 g          911 t
ORIGIN      
        1 tgcagtatta aacatttgca cataataaaa gggtaaatct ccctgattcc ataaggaacg
       61 ccaatcatct atcaacattt tcatcatgta gcgatattcc aatggtcttt ttgtgttgct
      121 ttctccctga taccatataa agcctttgat aggaaatcct ttcaatggat tgatttgccc
      181 attaaaatta acggaatgac tattattgta actactggct gtcaaaaaag cattcctacc
      241 ctgaattata caatactccg gttcagccaa tttccaggga ctgatccatg tttctatttc
      301 cgtaccacct ctgtaggctt gcatgattcc gatgggtatt ccggtttttt catggatttc
      361 acgtgcaaac acgtaagcta cggccgaaac attatctgct accttactgc tgttacatac
      421 aaaccaggga gaagatgatc cgcttaccct gtcagatggt tcattctgaa gattgtaagc
      481 tgcccggaaa gaacgaatct gagaataatt gtcagcttca ctgatgtatg tatccgcctc
      541 taataaatga ttgacctttt tctccatatt cgactgtcct ccggcaaacc atacatcacc
      601 aattaaaata ttttcgagct cctgcagggt ttcaggatac ccttttatac tgaattttaa
      661 ttgacagggt tcagtagaaa ctgcctgagc aggcaattca acttcccact tattatctgc
      721 attcacttca accaaatctg tatataccga atctgagcgt accaattcaa caagaaccgt
      781 atctccctga gcagcccacc catgcaattt caaaggaaca tcacgctgaa gtaccatatc
      841 ggaactgata aaacgtggta actccagata atgattttcc atatcatttc ctgtaatggc
      901 atcccaactg gtagctattc gcacagcatc tattgtaaaa acacctgaat tattacgggc
      961 ctgcatggca aagacattgg cagatacatc attcccggca tcagacaatg gtcccacaac
     1021 cggaagtccg ggtgttgaag cttctaaatc cggatcaatg aataaactaa cctgatcatt
     1081 tgcactgccg gaaacaaaat catattgaac aaccaccaga taagtactac caaactggta
     1141 aggtgtacct gaagtatagg ttactgtacc ggtagtggtt ttgaccagtc caaactgaaa
     1201 tccctctcct tcagctttta aataaatcct gcctctaaag gcattttggc tgaagaaacc
     1261 tgcaaaaaac tccccactcc ctgcagaact cacattaatc aaaaatgaat aataatacga
     1321 gccggttaat gttccggtaa aatttttctg aacccggtcg cttaatgggg aaaaatgagc
     1381 agccaatcca acacctgacc cagggtagcc tgtatagctt aaaccctgtt ctgagaccgt
     1441 cactctgttt gtttctgctt ttccttgtac ccaatcaccc tgacctgcca aatctgtccc
     1501 gaaaacatat tcgaagtttt cttctaacag aagtgctgag gattgagcaa ataaatatgt
     1561 ccctgataag tagaacaaca gtaccaataa acctgtttta aaatacataa acattcgttt
     1621 tttgaaatac tttcaagttg ccaaatcata ttttttcaat cctcaactct ccgtcagtga
     1681 gtttgacttt cagaatagtt tccttcacac ctttttccat ttttcatgga caagatggta
     1741 atggacttta cttaaccaat attttctcta tacttgtttc cccatttgaa gattgcacct
     1801 tcattacata tattcccgga ttgacaatat gtatgtctgc ctgattttta acctgaacgg
     1861 cctttacaac agaaccggtt aaatcataca gagtaagcaa tgcagcagga gcagtaattt
     1921 gaattccacc ctggatgtaa ttccaattaa ctgatgactg atttttattt acattgattc
     1981 ctacacccat atctgtttca taagttttta cactaaaatt atctacccag aaattgaacg
     2041 aagtcgtacc tgaatactta tgtgtaaagc caaaatgact catatctgaa cttgtatggg
     2101 tattgtcaac aacagaccct gccaacacat atcccgtatc tgtaagcgga tccgaaaaag
     2161 atcctccgga agcgggccca tcatttcgat catataattt ccaggtattt gatgaaggaa
     2221 gatataccaa tttaatactc atgtacactc tattatctgt caaagtttgc ccatgttgta
     2281 atatcgttat attatctcca ttggtgagtc cgttactaaa tttaaccaga cgataatcta
     2341 taggggaagc tccaccatta ataattgcat atccattggc aactgataaa tcagaggagc
     2401 tggcaaccaa tactactcca attcccctgc tagaagcatc atcaataccg gttagacgac
     2461 cattatagtt ttgtcgcaca ttaaatctcc aaaccagaga atccgcctca atattattta
     2521 attttgaatt gaacggagaa ctgtaaccag ctaaaccggc cgtcacttgt gagcgcgcat
     2581 tttcaccact cacattgggt aatcttaata agtcgccgtc ttgtataatc ggcacagccg
     2641 taccggtaac tgtcgttgtg tacgttactt ctggactacc tccgggagaa atatcaaccc
     2701 tattaaagtt atccgaaaac acagtagttt cagtaaccga ccctgctgcc actacaccag
     2761 taataattcc gaatgccatt agcaaggcaa acattgtttt gttacgtaaa tttgttttca
     2821 taattataat ttttaaagtg aataaatttt tagtgaatta ttattatttt tatttgattc
     2881 gtttgattta cattaaaaga taaaacataa atccccttat taaccggaat tttcacagat
     2941 tgtccggctt gcagatttcc gtattgtaac acacgtcccg aaaaatcggc tatatt
//
LOCUS       DAOSPX010000370         2989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_29853_length_2989_cov_3.470007, whole genome shotgun sequence.
ACCESSION   DAOSPX010000370 DAOSPX010000000
VERSION     DAOSPX010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29853_length_2989_cov_3.470007"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PLZ75_13285"
     CDS             <1..878
                     /locus_tag="PLZ75_13285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOS73412.1"
                     /translation="PYEYSLNGGDFRSSGIFTDLSAGSYIVTIRDAQPEEYHIMVILD
                     EPATAVSVETRVINNICHGSNRGIAIAIADGGTGPYEFSWNSLPVQKNDTASGLAAGT
                     YTVTVDDANGCRVTAFAVLTDPPPLTLGVITTDADCPDTYEGSAEVTVSGGNAPYNAF
                     WEDGNTSFSRTEMLPGTYRLIVTDADGCVAQEDIVIGYSGTFGCLEIPQVITPNNDGF
                     NDEWRIRNIEMYPDAEVLVYTRWGKLVYRSRNISADPWDGRFNGRLMPTDSYHYILYL
                     NDGSRPRTGVISIIR"
     gene            932..1903
                     /locus_tag="PLZ75_13290"
     CDS             932..1903
                     /locus_tag="PLZ75_13290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOS73413.1"
                     /translation="MAVLVSFSRELYSQQVPMYSQYIMNGYLVNPSFAGRDGYSTITL
                     TAREQWVGLAQNPATYAASFQTRLLKNSYISKSTEVRKKLVKPSRGSRVGMGGYIFND
                     NNGIMRRTGLQAIYAYHIPIGRSEESRSSLSFGLAMTAYQFAVNTDNLIYDIDDPFLN
                     TYKKSVFIPDFNFGTSYTSSRYYVGFAMTNLLRGSLLFGDVENSRRGELGHFYLTGGV
                     KFPVAEDWIIEPSAFVKSSDMVFKSIQMDITSRVYYKNDYWAGLSWRTNDALILLLGL
                     KYDRFYFAYAFDFTLTDIRRQSNGTHELTAAVKFGESARRYRWINSF"
     gene            1923..2966
                     /locus_tag="PLZ75_13295"
     CDS             1923..2966
                     /locus_tag="PLZ75_13295"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73414.1"
                     /translation="MIKRRLAVLILFLFHFPWSSHMLFPRPSPGEPLLLMFYNVENLF
                     DIYDDPDTNDDEFLPGSPRRWNMRKYKSRINSLYKVISAAGSWTPPGIVALCETENRR
                     VLEDLVYGTNLAKYNYEIIHEDSPDPRGIDVCLIYRKEIVEVLFWEYLVPKTYTAGTF
                     TSRSVLYAKCLAGGDLIHIFVNHWPSRRGGVLAGERLRKDIAVMLRDKADSVALSDAG
                     SKIVITGDFNAAPDDNIVRMIQDPYSSGLAMINLSADTPGGQGTYRYRGTWETIDQFI
                     VSESLLDSPGGLYTSREYYHIFSPDFLLKKDPVYPGMSPYSAWRGYKFQGGFSDHLPV
                     LLELRHRDHSQAE"
BASE COUNT          788 a          669 c          760 g          772 t
ORIGIN      
        1 caccttatga atacagcctg aacggaggtg atttccggtc ttcgggtatc tttacggatc
       61 tttctgcggg cagctatatt gtgacaatca gggatgcaca gcctgaggaa tatcatataa
      121 tggtaatcct tgatgaacct gccacagctg tcagcgtgga aaccagggtg ataaacaata
      181 tttgccacgg aagcaaccgt ggtattgcca tagccattgc agacggagga acagggccct
      241 acgaattttc atggaacagc ttgcctgtgc agaaaaacga tacagcttcg ggactggcag
      301 ccggcaccta tactgtaacg gttgatgatg ccaatggctg cagggtaaca gcctttgctg
      361 tcctgacaga tcctccgccg ctgacgctcg gggtgataac aacagatgcc gattgccctg
      421 acacctatga gggttctgcg gaagttaccg taagcggagg caatgcaccc tacaacgctt
      481 tctgggaaga cggaaacaca tccttcagcc ggactgaaat gttgccgggc acctacaggc
      541 ttatcgtgac ggatgccgac ggatgcgtcg cgcaagaaga tattgtgata ggttacagcg
      601 gtaccttcgg ctgtcttgag ataccccagg tcattacccc gaacaatgac gggttcaatg
      661 atgaatggag gatcaggaat attgagatgt atccggatgc tgaagtcctt gtttacacca
      721 ggtggggcaa gcttgtctac aggtcgagga atatatccgc tgatccctgg gacggaaggt
      781 ttaacgggcg gctgatgcct acggattcat atcattacat attgtatctg aatgacggct
      841 ccaggccgag gacgggtgta ataagcataa tcaggtaaag aggaatatta tgaatatgat
      901 acacatgaaa tacgtcagat gcttattgct gatggctgtt ttagtttcat tcagcaggga
      961 attatattca cagcaggttc ccatgtacag ccagtacata atgaacggat atcttgtcaa
     1021 tccctccttt gccggccgtg atggctattc taccataact ctgaccgcca gggaacagtg
     1081 ggtgggactt gcgcagaatc cggcaaccta tgctgccagc tttcagacca gacttctgaa
     1141 gaacagttat atatcaaaat ccaccgaagt caggaaaaag ctcgtaaaac cttcacgcgg
     1201 aagcagggta gggatgggtg gctatatttt caatgataat aacggcatta tgagacgtac
     1261 cggtctgcag gcaatatatg catatcatat tcccatcggc cggtccgaag aaagcaggag
     1321 cagcctttcc ttcggactcg caatgactgc atatcagttt gcagtaaaca ctgataatct
     1381 gatatatgat attgatgatc cctttctgaa cacgtataag aaatcagtct ttattccgga
     1441 tttcaatttc ggtacaagtt atacttcgtc ccgttactat gtgggttttg ccatgaccaa
     1501 cctgctcagg ggatcccttc tcttcggtga tgttgaaaat tccagaagag gagagctggg
     1561 tcacttctat cttaccgggg gagttaaatt ccctgtagcc gaagactgga taattgaacc
     1621 ttccgcattt gtcaaatcct cggatatggt tttcaagtcg atccagatgg atattacatc
     1681 aagggtttac tacaaaaatg attactgggc aggattgtcg tggaggacta acgacgccct
     1741 cattcttctg ctgggcctga agtatgacag gttttatttt gcctatgcct ttgattttac
     1801 cctcacggat ataaggcgtc agagcaacgg gacacatgaa ctgacggcag cagtcaagtt
     1861 tggtgaaagt gcacgccggt acagatggat caattcattc tgaaatagag ccccggattg
     1921 atatgataaa gagaaggctt gctgttctca tcctgtttct cttccatttc ccgtggtcat
     1981 cccatatgct gtttccccgg ccttcccccg gtgagccgct gctgctgatg ttttacaatg
     2041 ttgagaatct cttcgatata tatgatgatc cggataccaa tgatgatgaa tttctcccgg
     2101 ggagcccgag gagatggaat atgagaaaat acaaatcgag gatcaattcc ctgtacaagg
     2161 taataagtgc cgcaggcagc tggacacccc ccggaatagt agctttgtgt gaaacagaaa
     2221 acaggagggt tctggaagac ctggtgtacg gaacaaatct tgcgaaatac aattatgaaa
     2281 ttatccatga ggactctccc gaccccaggg gaatagatgt ctgtcttatt tacagaaagg
     2341 aaattgtaga ggttttattc tgggaatatc ttgtgccaaa gacctataca gcaggaacct
     2401 ttacaagccg gagtgtgttg tatgcaaagt gccttgcagg aggagacctc atacatattt
     2461 ttgtaaacca ctggccgtcg agaaggggag gtgtgctggc cggagagcgg ctgaggaagg
     2521 atatagccgt aatgctgagg gacaaggcag actcggttgc cctttcggat gccggaagca
     2581 agatagtgat cacgggtgat ttcaatgcag ctcccgatga caatattgta agaatgatac
     2641 aggatcccta ttcttccggt ctggcgatga tcaatctctc cgctgatact cccggggggc
     2701 agggtaccta caggtatcgc ggaacctggg agacaataga ccagtttatc gtttcagaat
     2761 ccctgctgga cagccccggg ggcctttaca catcaaggga gtattatcac atattcagtc
     2821 ctgattttct gttgaaaaag gatcccgttt atcccggaat gagtccctat tcagcctggc
     2881 gcggctataa atttcaggga ggtttcagtg atcatctccc tgttctgctt gaactcaggc
     2941 accgggatca ttctcaagcg gagtgagtcc gagttccctg ccttttctg
//
LOCUS       DAOSPX010000371         2988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_29862_length_2988_cov_4.952949, whole genome shotgun sequence.
ACCESSION   DAOSPX010000371 DAOSPX010000000
VERSION     DAOSPX010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29862_length_2988_cov_4.952949"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1250)
                     /locus_tag="PLZ75_13300"
     CDS             complement(3..1250)
                     /locus_tag="PLZ75_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOS73415.1"
                     /translation="MRLTSPAGRPAFFRLLVIAAFIGFSAASCKDPSTANKSGGSNYY
                     FPEGYLDQPDSIPAFWITTNEEIISFLKTKVRTGKVERIGTSAGGRPVMAVMYGSPRK
                     GGGTTTFSGACGIGNIAPYRGQDNDKTVYLGLGGVHGFETEGIMGIVNLISVLEEGRD
                     LNGKEWPEITSVLRNVDRIILIPLLNPDGRARVPVIMETHKGASRNSYLAHEYLNTGG
                     KPDGSLIGWPDVKEFIPMDFSKVGFPGGYPNDAGVNLMHDDFFGKLQPENQALFDLAA
                     REKPDIIINMHTGAPGNNYFMRMHRPVCEAALEPVYDSLYVMAHTGLALRGLQSTNDP
                     DIEADPSKSPRGVYNLDCALNLHCGALSVVVEAPSHGFSGTDRSGKPVLQTPEMILNA
                     ELVVHLEAMKFLAGSGGRSRWKI"
     gene            complement(1268..2374)
                     /locus_tag="PLZ75_13305"
     CDS             complement(1268..2374)
                     /locus_tag="PLZ75_13305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOS73416.1"
                     /translation="MKNKFLIFIALCLTMTGHAQNKAVSGENPVKFYDHNFFLTEGTV
                     FSGTVKENLYDRFPLSYKDKVREAVWNLSKNSAGISVRFLTNSTSLRVRWELLNDLRM
                     NHMAETGIKGIDLYFKNGTEWQYLNTARPSGKSNDYLLIDNMTPVMREFRMYLPLYDG
                     VTRLEIGIDSLGDMVKPRESTRKPIVFYGTSITQGGCASRPGMVHTSIISRKLDVDCI
                     NFGFSGNGRMEKPLAELISGIDASFYVLDGTNNMTPEQIHENAIQLVEIIRKNHPVTP
                     IVFVEGMLNDKSFLDEKTRNLENAKNTALKQEFEKMKRQGFRNIHYIEQAGGKGYDSE
                     ATVDGIHLTDLGFSRYADFLIREFRKIKLIDRKK"
     gene            complement(2478..>2988)
                     /locus_tag="PLZ75_13310"
     CDS             complement(2478..>2988)
                     /locus_tag="PLZ75_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS73417.1"
                     /translation="TYYSRVFKIDTMTFRFIFLDNGYYSTQEISDGELNFSVDPYQLR
                     WLDAQLKASPSDVEIIFMHIPLPNGKPAENEILTEAMSDYSQKTEVYNLFSVLENNSS
                     TAMIFGGHNHINLINRYKLPNGNLLTQVRTGAFGNDTANWRMIRITKDKILVSRAGST
                     VTEYTVPLY"
BASE COUNT          779 a          722 c          617 g          870 t
ORIGIN      
        1 atttaaattt tccatcttga tcttcccccg cttccggcga gaaatttcat tgcttccaga
       61 tggaccacaa gttccgcatt aaggatcatt tcgggtgtct gcaaaacggg tttgcctgaa
      121 cggtcagttc cggagaaacc atgggaaggt gcctcaacga ccacgctcag tgccccgcag
      181 tgaagattca gtgcacaatc caggttatag actccgcgcg gcgattttga cggatctgct
      241 tcaatatccg gatcatttgt actttgcagt cctctgagag ccaagcccgt gtgagccata
      301 acataaaggg aatcatatac aggctccagt gctgcttcgc atacaggccg gtgcatcctc
      361 atgaaataat tattcccggg agcacctgtg tgcatgttta ttataatatc cggtttctcc
      421 ctggcagcaa gatcgaaaag agcctggttc tccggctgca gttttccaaa aaaatcatca
      481 tgcatcaggt tcacgcctgc atcattcgga tatcctcccg ggaaaccaac cttcgagaaa
      541 tccattggaa taaattcctt tacatccggc cagcctatca gggagccatc aggtttcccg
      601 ccggtgttaa ggtattcatg ggccagatat gaatttctgg aagccccctt atgtgtttcc
      661 attattaccg gtacgcgtgc ccggccatcc ggattaagaa ggggtataag aattatcctg
      721 tcaacattcc tgaggacaga agttatttca ggccactctt tcccgttcag atcccttcct
      781 tcctcaagta ctgagatcag gttaacaatt cccattatac cctctgtctc aaaaccatgt
      841 acccctccga gcccgagata aacggtttta tcattatcct gaccacggta cggggcaata
      901 ttccctattc cgcaggcacc ggaaaaagtt gttgtgcctc cgccctttct gggtgatccg
      961 tacataaccg ccatcacggg acggccgccg gccgaggttc ctatcctttc aaccttccct
     1021 gtcctgactt tagtcttgag gaaggaaatt atctcctcat tggtggtaat ccagaaagcc
     1081 ggaatactat ccggctggtc cagatatcct tcaggaaaat aataattact gcctcctgat
     1141 ttgttggcgg ttgaaggatc tttgcatgaa gctgccgaaa agcctatgaa agcagcaatt
     1201 accaagaggc ggaaaaaggc aggccggcct gccggcgaag ttaaacgcat gaatattgaa
     1261 tttactgtca tttcttcctg tcaatcagtt ttatttttct gaattccctg atcaggaaat
     1321 ctgcataacg tgagaatcca agatcagtaa gatgaatacc gtccacagtg gcctccgaat
     1381 cgtaaccttt tccacctgcc tgttctatat agtgaatatt tctgaatccc tgtcttttca
     1441 ttttttcaaa ctcctgcttc aatgcagtgt ttttggcatt ttccaggttt cgcgtctttt
     1501 cgtcaagaaa ggatttatcg ttcagcatgc cttcaacaaa tactatcgga gtcacaggat
     1561 gattcttacg tattatttca acaagctgaa tggcattttc atggatctgc tccggcgtca
     1621 tattgtttgt accgtccaga acataaaacg atgcatcaat cccggaaata agctctgcca
     1681 gaggtttctc catcctcccg ttgccactga acccgaaatt aatacaatca acatccagtt
     1741 tccttgaaat aatactcgta tggaccatac caggccggga agcacatccg ccctgggtaa
     1801 tactggtacc ataaaacact attggtttcc tggtgctttc cctgggctta accatatcac
     1861 caagactgtc aattcctatt tcaagcctgg taaccccatc ataaagaggc aggtacattc
     1921 tgaactcacg cataacagga gtcatgttgt caataagaag atagtcattg ctttttcccg
     1981 aaggacgtgc ggtattaaga tattgccatt ctgttccgtt tttgaaatag agatctatgc
     2041 ccttgatacc tgtttcagcc atatgattca tccgcaagtc gttaagcagt tcccagcgga
     2101 ctctcaacga tgttgagttt gtcaggaacc ttactgatat tcctgccgaa ttttttgaca
     2161 gattccagac tgcttccctt accttatcct tgtaagatag aggaaacctg tcatacaggt
     2221 tctccttcac cgttccgctg aaaactgtcc cttcggtcag gaagaaatta tgatcataaa
     2281 attttacggg attttcgcct gaaaccgctt tattttgggc atgccccgtc attgttaagc
     2341 ataaggcaat aaatatcagg aacttatttt tcataattta tttctaattt ttgttgagcg
     2401 ggttctttac ttctatcttt tcataagcgg caaaggaaca aaagcaacag ttgccaaaca
     2461 agactgcaac actttggtca gtacaggggg actgtatatt cggtaacagt actcccggcc
     2521 ctgcttacca ggattttgtc ctttgtgatt ctgatcatcc gccagtttgc tgtatcattt
     2581 ccaaaagccc cggtcctaac ctgtgtgagt aaattaccat ttggaagctt atacctgttg
     2641 atcagattaa tatgattatg gcctccgaaa atcatggcag ttgaggaatt attttccaga
     2701 acactgaaca gattgtacac ttcagttttt tgtgagtaat ccgacatcgc ctcagtgagt
     2761 atttcatttt cggccggctt tccgttggga agaggtatat gcatgaagat tatttccaca
     2821 tccgaggggg aggccttaag ctgggcatcc agccaccgca gctggtaggg gtcaacagag
     2881 aaattcagtt ctccgtcaga tatttcctgg gttgaataat agccgttatc aaggaaaatg
     2941 aaacggaaag tcattgtatc aattttaaaa acacggctgt aataggtg
//
LOCUS       DAOSPX010000372         2976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_30025_length_2976_cov_3.786375, whole genome shotgun sequence.
ACCESSION   DAOSPX010000372 DAOSPX010000000
VERSION     DAOSPX010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30025_length_2976_cov_3.786375"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLZ75_13315"
     CDS             complement(<1..522)
                     /locus_tag="PLZ75_13315"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73418.1"
                     /translation="MQIKEFHYDFKALSGFQPLVMDETQSEIRVKGGTFEYTISKMNG
                     LFSSARVLGDEWLAGPLPDLWASPANDPRTMKYFARLTSSAEVKITEPGPDRVVIASR
                     GVFTAEDSTTLPLNYNLLYTFDIDGTARVDLKVSADEKVVLRCLTLLESRISDACSFV
                     SHEYDLGNGSRTCS"
     gene            complement(874..>2976)
                     /locus_tag="PLZ75_13320"
     CDS             complement(874..>2976)
                     /locus_tag="PLZ75_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOS73419.1"
                     /translation="DPPRTDIEDIAAISEAVDRYLRTRTRLGIPAIQLELGGMHGMLA
                     RGASNFPQVIGQGSTWNIELIREMARVIAYEGSLIGCNQVFSPLFDIARDPRYGRIEE
                     CFGEDPYLVAEMGKAFVIGIQGDPSVTKHHIPDGHLISTAKHYAGYSTPVAGINIAPV
                     EIGPRDFRDLYLYPFEKAVKEANVYSVMPAYNEVNGIPVHASEYLLRDVLRGEFGFRG
                     YVFADYGAVSMLHNTHKITADRAETALVALKAGVDQEGSNWAYSELISLAKNDKNIEA
                     LVDEAVRNILGVKFRAGLFDKAYRKPENLTGLCHTEESVKLARKIAEESIILLKNGNS
                     LLPLKAEQLKSVAVIGPNANQVQYGDYSITKDNISGVTILQGIKNLAGNRLKINYAKG
                     CGITDPDKSGFREAVKAAKKSDVVILVIGGTSMSLSGTGWGELAAEGAYPTCGEGYDR
                     SELTPPGVQQELIRAIYKTGKPVVLVMVHGRPYSVKWEKENLDAILEAWYPGEEGGNA
                     VARVLFGEAVPSGKLTVSVPQSVGHVPVFYNHKPTGRGYYKKPGTPESPGRDYVFSST
                     EPLWSFGYGLSYTEFEYSDLKIARKELTDRDSIELSVKIRNTGKVNGKEVAQLYINDM
                     ISSVTTPVKVLKGFKKPEIKAGESVTVNFTLPCRELGLWDRNMNYVVEPGEFEIMIGA
                     SSDDIRLRDVIRYHPASD"
BASE COUNT          703 a          766 c          631 g          876 t
ORIGIN      
        1 gctgcatgtt cttgaaccat tccccagatc atattcatgg ctgacaaaac tacaggcatc
       61 cgatatccgg ctttccaata aagtcagaca ccgtaaaact actttttcat cagcactgac
      121 ttttaaatct actcttgccg tcccatcgat atcaaaagta taaagcagat tgtaatttaa
      181 aggaagtgtt gtactatcct cagctgtaaa tacaccccgg gatgctatta ctacccggtc
      241 aggtcccggt tcagtgatct taacctctgc agaagaggtt aaacgggcaa aatacttcat
      301 ggtgcgggga tcatttgcag gagaagccca aaggtcaggc aacggacctg caagccattc
      361 atctcccaat acccgggcag aggagaaaag accattcatt ttgcttatag tgtactcaaa
      421 tgtaccaccc ttaactctga tctcagattg ggtctcatcc ataacaagag gctggaatcc
      481 ggaaagggct ttaaaatcat aatgaaattc ttttatttgc atcccgtttt cattgatggg
      541 tcgggaatga cctgttgcaa aagagccggt aacacccgta agtgctaaac ccccgtatgc
      601 ggccttatga agaaaatttc tccttttcat tgtttccgaa actttttgtt gattatccga
      661 aacaaatata aaccctcatc cggcaaaagg ctatttaagt ttatagaatt ttatactgta
      721 cagacagggc caaccgtcca ggatggaatc catacccgat attcctgccg gccggtcatc
      781 ataatggcta atatttttcc tgaaagattt tgctgatcag tcaaacagga ttatggttat
      841 agtattgcct gactgtctct gatccgcaca attctaatca ctggcgggat ggtaccggat
      901 tacatccctc agccttatat cgtcggagga tgctcctatc attatttcaa attcgccggg
      961 ttccaccacg taattcatgt tcctgtccca caggccaagc tccctgcatg gaagcgtgaa
     1021 attcacggtt accgattctc ccgccttaat ctctggtttt ttaaatccct tcagcacttt
     1081 cacaggggtg gtaacagaac tgatcatgtc attgatatag agctgggcga cttcctttcc
     1141 atttaccttt ccggtgtttc tgattttcac gcttaactca atactgtccc tgtcagttaa
     1201 ttcttttctg gcaatcttga ggtcggaata ttcaaactct gtgtagctca gtccgtagcc
     1261 gaaagaccac aggggttcag ttgatgaaaa gacataatca cgtccgggac tttccggtgt
     1321 acccggtttt ttataatatc ctcttccggt tggtttgtga ttgtagaata caggaacatg
     1381 tccaacgctt tgcggaacag aaaccgtgag ttttcctgag ggcactgctt caccgaacaa
     1441 tacccgtgcc acagcgtttc ccccttcttc accagggtac catgcctcga ggattgcatc
     1501 caggttttcc ttttcccatt tgacggaata gggcctgcca tgaaccatca ccaggactac
     1561 gggcttcccg gttttgtata ttgccctgat cagttcctgc tgtactcccg gaggtgtaag
     1621 ttccgatctg tcatatccct ccccgcaggt gggatatgcc ccctcggctg caagctcacc
     1681 ccatccggtg ccgcttaaag acatgcttgt gcctccgatg accagtataa cgacatcact
     1741 tttttttgcc gctttcactg cctccctgaa tccgctttta tcgggatcgg ttattccgca
     1801 tccctttgca taatttattt tcagtctgtt gccggccagg tttttaatgc cctgaagaat
     1861 tgtaactccc gagatattgt cttttgtaat gctgtaatcc ccgtactgga cctggtttgc
     1921 attggggccg ataactgcaa cagatttgag ctgttctgcc ttcaggggca gaaggctgtt
     1981 tccgttttta agaaggatta ttgactcttc agcgattttt cgcgcgagtt ttaccgactc
     2041 ttcggtatgg cacagaccgg tcaggttttc gggttttcta taggctttgt caaacaatcc
     2101 cgccctgaat ttcaccccga gtatatttct tacagcttca tccaccagtg cttcaatatt
     2161 tttgtcattt tttgccaggc ttatcagttc cgaataagcc cagttgcttc cttcctggtc
     2221 aactcccgcc ttcagtgcca caagggctgt ttcggccctg tcagcggtta tcttatgtgt
     2281 attgtgaagc atgctaactg cgccgtaatc ggcgaacaca tatcccctga agccgaattc
     2341 cccgcgcagc acatcgcgga ggaggtattc actggcgtgt acgggtatcc cgttaacctc
     2401 attataggcg ggcattacgg aatatacatt ggcctctttc accgcttttt caaaaggata
     2461 taaatacagg tccctgaaat ccctgggccc gatctccacc ggagcaatat tgatgcctgc
     2521 cacaggtgtg ctgtatccgg catagtgttt tgcagtactt ataagatggc catcggggat
     2581 gtgatgcttt gttacagagg gatcaccctg tatccctatt acaaacgcct tgcccatttc
     2641 ggcaaccaga taaggatctt caccgaagca ttcttctatt cttccatagc ggggatccct
     2701 tgcaatgtcg aataatgggg aaaaaacctg gttgcaaccg attaaactgc cttcatatgc
     2761 aataacccgg gccatttccc tgatcagttc tatgttccac gtgctgccct gtccgattac
     2821 ctgggggaaa ttgcttgctc cccttgcaag cattccgtgc attccgccca gttcaagctg
     2881 aattgcagga atgcccagcc tggttcgggt cctgaggtat ctgtcaacgg cttccgagat
     2941 agcagctata tcttcaatat cagttctcgg gggatc
//
LOCUS       DAOSPX010000373         2974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_30047_length_2974_cov_4.486811, whole genome shotgun sequence.
ACCESSION   DAOSPX010000373 DAOSPX010000000
VERSION     DAOSPX010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30047_length_2974_cov_4.486811"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..545)
                     /locus_tag="PLZ75_13325"
     CDS             complement(234..545)
                     /locus_tag="PLZ75_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOS73420.1"
                     /translation="MKTKMSLLILLSSSFSLFSQQNIFISPFGLPDQRGVVITDVREG
                     SLADLSGFRRNDVIRAVNGNEISSIEEIYAFSEENWWLGGMMLKIYRNQKEEEKRIAI
                     K"
     gene            complement(952..>2974)
                     /locus_tag="PLZ75_13330"
     CDS             complement(952..>2974)
                     /locus_tag="PLZ75_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS73421.1"
                     /translation="VGFQTVEIPVGTRSRFDIALQPDVLALDDVVVTATKTIRNLTEV
                     PSRINVVSSKTIEASPALLVEDILRFTPGVNIDRSVGIYSSSNPSVTLRGLSGDEQSR
                     TLVLMNGVPINASDGGSVNWNKINQYDVERIEVFKGPGSSLYGNNAMGGVINIITKVP
                     GKPQEIYGSISYGTFNTIRQDLALRIRSEKGFYGSLSQNLIKSDGYLSIPEEKRTPYD
                     IKRNYEAIDVSAKAGYDKNKWLKTELQYDVYRDRRGEGTRVYIPLGNYRRFDTDLLRG
                     VLKGGDDNTQYDMSVYYQMVNYTDLNESVKGSGAYQRYDVNSYRKDWGALFNFTRQLS
                     ENNSLTGGLEYKNGSVKGGDYYQTAPYDTVYNEGVTQIYAGYIQDEHAFFDEKVRLVA
                     GLRYDYVAFSDGYYYSTSPWGDPPVLKDHNWSEFSPRIGLRFNFIKELSAYLSYSHGF
                     RASILDDLTRTGYMWVGPKYANPDLGPESIDNYEIGADLMLSRFKLSASGYYALGDDF
                     LYYVNSGDELSPGRPIYRRENVSSVSLKGAEAEISYYPLSNLRFMASYTYSHSTINKF
                     DVHPDLVNKFLTYVPKNRFSLAMFWQNRIVNVNIQALYKSAQFSDDANTEANIIDAFT
                     TVDLQLSRKLFGNFSGMIDIQDIFDNQHMQTLNSLSPGRRITAKVAFKF"
BASE COUNT          800 a          695 c          605 g          874 t
ORIGIN      
        1 ggggtgaaaa tgctcatttc acactatgta gatatacccg actggctctc attgggtgtt
       61 gttgtactgg ccctggctgc aggtatgatt gcttcaaaag taaaaaaaaa tcatatcgca
      121 taatcaaccg gaatgtaaat acactaataa gaatctgttt ccaaataaaa cctgaatcag
      181 atgaaatgtc tgttgttttt attttccgcc gccaccagga gataatcctg ttattatttt
      241 atggctattc tcttttcttc ttccttctga ttcctgtata ttttcagcat catcccgcct
      301 aaccaccagt tttcctctga aaaggcatat atttcttcta tggaagagat ttcattgccg
      361 tttacagccc gtatcacgtc atttctccgg aatcctgata aatctgcaag actgccttcc
      421 ctgacatcgg taataaccac tcccctctgg tcaggcaggc caaaggggga aataaaaata
      481 ttctgctggc tgaaaagcga aaaagaggat gacaacagga ttaacaatga cattttagtt
      541 ttcataccag tggtcaatta tatttttaat tcaattcact gttgaacaat gtttacagga
      601 ccggtccgga atgcaggttt caaaggaaca tctgaaaaat attgttcatc acttagcaac
      661 ccgccagccc ggttatttat ccgatgagtt ccggacaaat tattccctgc tcctcaaaag
      721 taatcgaatt cctctgagcg gcaagatttc tgttaagcgg attttctgtc cctggatctg
      781 gttgtccggt ctccctcagt acaggcattt ccggaagttc acaccggaac gtatctgaat
      841 tattatactc tgtcctccaa ttgggggaca gagtgcattc atcatgattc aatccggtga
      901 ccaacatgac agcaatatgt tcaccggatt atttttaaag aatttatcta tttaaaactt
      961 aaaagccact tttgccgtaa tccttctccc cggagaaagg gaattcagtg tttgcatatg
     1021 ttgattgtca aaaatatcct gtatatcaat catccccgaa aaattgccga atagtttgcg
     1081 cgataactgc agatccacag tagtgaatgc gtcaatgata tttgcttctg tgttcgcatc
     1141 atccgaaaac tgcgcacttt tgtaaagtgc ctggatattt acattaacga tcctgttttg
     1201 ccagaacatg gccagagaga aacggttttt tgggacatag gttaggaatt tgtttaccag
     1261 atccggatgg acatcgaatt tgttaatggt tgaatggctg tatgtatatg aagccatgaa
     1321 ccggaggttg gataacggat aatagctgat ttccgcttca gcgcctttca ggctgacgct
     1381 gcttacattt tccctgcggt atattggtcg tcccggcgac agttcatctc cggagttgac
     1441 ataataaagg aaatcgtctc ccagggcata atatccgctt gcagaaagtt taaacctgga
     1501 taacatcaga tcggctccga tctcgtagtt atcgattgac tcaggaccta aatcggggtt
     1561 ggcatatttt gggccgaccc acatatagcc tgtacgggta aggtcatcca gaattgaagc
     1621 cctgaaaccg tgcgaatagg aaaggtaagc gctaagctcc ttgataaaat taaaacgcag
     1681 cccgatcctg ggcgagaatt ctgaccagtt atggtctttc agcaccggcg gatcgcccca
     1741 tggtgaggtg gaataataat atccgtcgga gaaagcgaca taatcataac gtaatccggc
     1801 aaccagcctt actttttcat caaaaaaggc atgttcgtcc tgaatgtatc ctgcataaat
     1861 ctgagttaca ccctcgttat acacagtatc atagggggca gtctggtaat aatcgccacc
     1921 cttgacgctg ccgtttttat attccagacc acctgtaaga ctgttgttct cagatagttg
     1981 tcgtgtgaag ttgaacagtg caccccagtc tttccggtat gaattcacgt cataacgctg
     2041 ataagccccg ctgcctttca cactttcgtt caggtcggta tagttaacca tctgataata
     2101 tacactcata tcatattgtg tgttgtcatc accacctttc agcacgccac gcagcaaatc
     2161 ggtatcaaaa cgccggtaat tgcccagcgg tatgtatacc ctcgtaccct cgccgcgtct
     2221 gtcgcggtat acatcatatt gcagctcagt tttcagccac ttgtttttgt cataaccggc
     2281 tttggctgaa acatcaatag cttcataatt acgttttata tcatagggcg tacgtttctc
     2341 ctcgggaata ctgagataac cgtcactttt tataagattc tggctcagcg agccgtaaaa
     2401 tcccttttca ctgcggatcc ttaatgccag atcctgacgg atggtattaa atgttccgta
     2461 actgatgctt ccgtatattt cctgtggttt gcccggaact ttcgtgataa tgttaataac
     2521 acctcccatt gcattatttc cgtagagaga tgacccggga cctttgaaca cttcaatccg
     2581 ttccacatca tactgattga ttttgttcca gttaacgctg cctccgtcag aagcattgat
     2641 gggaacgcca ttcatcaata ccagggttct cgattgttca tcccccgaca aacccctgag
     2701 cgtaaccgac ggattagatg atgagtaaat ccctacactc ctgtcaatat tcacccccgg
     2761 ggtgaagcgt aagatatcct cgacaagcag agccggactt gcttcaatgg tttttgagct
     2821 taccacgtta attcgtgaag gtacttctgt caggttacga atagttttgg ttgccgttac
     2881 tacaacatca tccagagcta aaacatcagg ctgaagcgca atatcaaacc gggacctggt
     2941 tcctaccggt atttcaaccg tctggaaccc aaca
//
LOCUS       DAOSPX010000374         2974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_30049_length_2974_cov_4.061665, whole genome shotgun sequence.
ACCESSION   DAOSPX010000374 DAOSPX010000000
VERSION     DAOSPX010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30049_length_2974_cov_4.061665"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(601..1998)
                     /locus_tag="PLZ75_13335"
     CDS             complement(601..1998)
                     /locus_tag="PLZ75_13335"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOS73422.1"
                     /translation="MTDILAQKTVIIRPEASDEVLINPGIGFQTFQRFNGDELNEGLG
                     WTEGFPIQYQKFSGKLENKDHPMTTIAYFRVYWRYLEPEMGRFNWEMIDRALKTAHER
                     GQTLSLRIAPYGSGKEPDDPTDVPSWYRAMVGNKNEWMPDGQQGWRVDAEDPRYAGYF
                     GRMITELGRRYDGHPDLEYVDLSIIGFWGEGRGSDIITQNTREALVRAYTDNFKKTPL
                     IMLLTDVKTNKYGLSRANVGWRVDCIGDIGFPVKDKGNPGWAHHYNYYPQGIINFGME
                     DAWKKAPVSLEICHVLKQWKEERGFTEDDVNYVIDETLKWHISSFNAKSSAVPEEWKP
                     LIDRWLKRMGYRFVLRSFSYPEFVSPNGKLAFQSWWDNRGVAPIYKKFLLAVRLTNDK
                     RTEVMITDADITSWLPGDNLYDDAVFIPPDMPPGIYQLQIGIVDRQSHKPGVNLAIGG
                     RTPEGWYRLGKIEIK"
     gene            complement(2147..>2974)
                     /locus_tag="PLZ75_13340"
     CDS             complement(2147..>2974)
                     /locus_tag="PLZ75_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73423.1"
                     /translation="NLEETDPLALRIYYNAHRYGTGNVLYCQRVPAITGRTLALSGLF
                     QGYPVISHARTPEQRGLLERNALLLHAFGANRSIALNKFEMGEVLPSDGGASVPGVSL
                     YYRPDNGNLLICLSTFSQPASTVQLGPILKSIISRISGITGYGAVTGKSSYYIYVFED
                     KDLIGGSPLKPDQLSAYTINLPAENYRNIWLKPAGKQPSILYSLSNGDAPVEDFKAGR
                     REFSVTIPSRARDLAETVVLGAAPRSVKAHDREISFKAHKGWWEGEFPCNTTVRILY"
BASE COUNT          801 a          674 c          641 g          858 t
ORIGIN      
        1 aatcctactt cttcaataat agcgaacttt ctcagaaatc tgaaaaggta tatagctcta
       61 attggctgat atagagacta ttagctaatt gaaaattaat aactataact ataaaatcta
      121 tgaatattcg gttaatttct ttgaaaccct taattttggt taaatcgatt ttaaagatcc
      181 taatggatgg cagaactttt ctgtaggaga aagagaagta aagcgggagc ttcggaaaat
      241 actctggacc aaatatcaga ttaaagatga agagcttttt ctaagagcat acgattatat
      301 cagagaatat tattgatatt cagaaaaata actctgcagt tttttccgac aaagaggacg
      361 gattgttcaa aaaactttgt aataccgtgg gatggatgtt tgactcccaa aatattgcaa
      421 agcatattag cctattataa agaggataca gcggttattc tctgattgaa agacaggaag
      481 tccagtggct ccttcctata tctgaaagtc aaaatgcatc tgtgcttcac gattacaact
      541 ggtttaagga tgatctttta gatagttatg ataaagcagt ttataagcta tggtttgatg
      601 tcatttgatt tctattttcc ctaaccggta ccagccttca ggggttcttc ccccgatggc
      661 aaggttaacc ccgggtttgt gtgactggcg gtcaacaata ccaatctgca gctggtatat
      721 tcccggaggc atatcagggg gaataaaaac agcatcatca tagaggttgt ctccgggaag
      781 ccaggaagtg atatcagcat cagttatcat gacctcagtt cttttgtcat ttgtaagcct
      841 gactgccagg aggaactttt tatatatggg tgcaacccct ctgttgtccc accaggattg
      901 aaatgcaagt tttccgttgg gagaaacgaa ttcgggatag gagaaacttc gcaatacaaa
      961 acgatagccc atccttttca gccatctgtc gataagcggc ttccattcct ccggtacagc
     1021 cgaggatttg gcattgaagg aggaaatgtg ccattttaat gtttcatcaa taacataatt
     1081 tacatcatct tcggtgaatc ctctctcttc tttccattgt ttgagaacat ggcagatctc
     1141 caggcttaca ggtgcctttt tccatgcatc ttccattccg aaattgatga tcccctgggg
     1201 ataataattg taatgatgtg cccagcccgg attgcctttg tctttcaccg ggaaaccgat
     1261 atcacctata cagtccaccc tccaccctac atttgcccgg gaaagaccgt atttattcgt
     1321 tttgacatct gtaagaagca taatcagcgg tgtcttttta aaattgtcgg tatatgctct
     1381 cacaagtgct tcccttgtgt tttgtgtgat tatgtccgaa cccctgcctt caccccagaa
     1441 tccaataatt gaaagatcaa catattcaag gtcagggtgt ccgtcataac gcctgccgag
     1501 ttcagttatc attctcccga aatatcccgc gtagcgggga tcttctgcat caaccctcca
     1561 tccctgctgc ccgtcaggca tccactcatt tttgttccct accatggccc ggtaccatga
     1621 gggcacgtca gtggggtcgt ccggttcctt tcccgagccg tagggtgcaa tacgcaggga
     1681 aagagtctgg ccgcgctcat gagctgtttt cagtgccctg tcaatcattt cccagttaaa
     1741 cctgcccatt tcaggctcca gatatctcca gtaaacccgg aagtaggcga ttgtggtcat
     1801 cgggtgatcc ttgttttcca gtttcccgct aaatttctgg tactgtatgg gaaagccttc
     1861 ggtccaccca aggccttcat ttaattcatc gccgttaaag cgctggaagg tctggaatcc
     1921 tatgcccgga tttatcagga cttcatcgct tgcttccggt cttataatga ctgtcttttg
     1981 agcaaggata tcagtcactg ttaacggaat gagacaaata cagaataata agatcttgat
     2041 tttcaaactt ttcattttag ttaaattatc gtttcactca atttacaaag caaataaaaa
     2101 cccatatccg gataaaaggt acttagccgg cttttttagc ccgcagttaa tacagaatcc
     2161 tgacagttgt gttgcaggga aactctcctt cccaccagcc cttatgagcc ttaaagctga
     2221 tttcccggtc atgtgccttc acggacctgg gagctgctcc cagaacgacc gtttcagcaa
     2281 gatcacgcgc ccgggatgga atggtcaccg aaaattctcg tctgccagcc ttaaagtcct
     2341 caacaggtgc atctccgtta ctcagggaat agaggatcga aggctgcttc ccggcaggct
     2401 ttaaccagat gttacggtaa ttttctgccg gcagattaat tgtgtaagcg ctcagttgat
     2461 ccggttttaa ggggcttccg ccaataagat ccttgtcctc gaacacgtat atgtagtaag
     2521 aagatttacc tgttacggcc ccgtatccgg taatgcctga gatcctggaa attatacttt
     2581 ttagtattgg acctaactga actgttgatg ccggctgtga gaaggttgaa aggcatatga
     2641 gcaaattgcc attgtccggc cggtagtata atgatacccc gggaacggaa gctcctccat
     2701 ccgaaggcag aacttcaccc atctcaaact tgttcaatgc aattgaccgg tttgcgccaa
     2761 aggcatgcaa aagcagggca tttcgctcca gcaggcctct ttgttccggt gtacgggcat
     2821 gactgataac aggatatccc tgaaaaagtc cggataaagc aagagtacgt ccggtaatcg
     2881 ccggaacacg ctggcaataa agtacattgc ctgtaccata tctgtgagcg ttgtagtaga
     2941 tgcgtaaagc caggggatca gtttcttcaa ggtt
//
LOCUS       DAOSPX010000375         2972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_30072_length_2972_cov_4.791909, whole genome shotgun sequence.
ACCESSION   DAOSPX010000375 DAOSPX010000000
VERSION     DAOSPX010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30072_length_2972_cov_4.791909"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PLZ75_13345"
     CDS             <1..880
                     /locus_tag="PLZ75_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73424.1"
                     /translation="NNAWAAIPLTWYSVIDGRSDRLIADAVRENQEVMRWYGERNIPV
                     EVNESHQWSLRDAHDSLAVAMAFLAAYNAKNAGVRHYVSQYMFNNPPGTTAEMDIAKM
                     LAKDEMIAKLSGDSFRVFRQVRAGLAHFSPDPSFAQGQLAASAVISLALKPHILHVVG
                     FSEGDHAIFDDELVQSCNIAHGVLHNTLYGMPDMTENKKVAARKNELISEAGVLLEEL
                     YNRGKNKSQDPWSDPLVISDAVRDGLLDTPHFQGNPHLCGRIVTRLIDGAWYATDRDT
                     GEKMSEWDRIKKLKGK"
     gene            1025..2359
                     /locus_tag="PLZ75_13350"
     CDS             1025..2359
                     /locus_tag="PLZ75_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOS73425.1"
                     /translation="MAGIAGIAADNASEEVLEMLCVMRHRGKTRLSVFENEGTTAGII
                     WNEPEDDSVKEFLDAGEAGYYNSPGNYAVVRPDNGSFTMRRDELGSAPLYYGSDNDGN
                     ICFASEIKALLPITSNIIELSPGHVYEDDKLRSYFGIQTGKKTEKSPDRIASELSGLL
                     DKAVSDCLTSEDIGSFLSGGLDSSVICALATRHLKKLKTFAAGLKGAPDLEHAREVAR
                     YLQTDHYELIITPEKLTAILAEVIYHLESFDALLVRSSLTNYLVARLASEHVAEVFSG
                     EGGDELFAGYGYLKSIPFEHLENELVRITNTMHNTALQRVDRSAAAHSLTPHMIFTNP
                     EVVKFAFTIPVEYKIHGTTEKWILRKAMEGRLPSGILRRPKVKFWEGAGVKELIAVYA
                     EKNISDNDYRTERDLKNGIILGSKEELLYYRIFREHFGSDIDLSFMGRTITH"
     gene            complement(2362..>2972)
                     /locus_tag="PLZ75_13355"
     CDS             complement(2362..>2972)
                     /locus_tag="PLZ75_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73426.1"
                     /translation="LISPSYTSALAQAIQVPGISGMENNMVIFEYDKENPENLGEIIE
                     NTGLIAAGDFDICLLASSRKEMIFRNGIHVWLDFHYPENSNLLILLSFIISGHPDWRK
                     SNIKLFGTCKNGEEEEARLNLTDLVRTGRLPITEKNIEIVQEEPGMTLKNIINKKSGL
                     AALTMIGFNTQILKRVGESFLDGYEETGTILFACSHNQKEIT"
BASE COUNT          864 a          611 c          741 g          756 t
ORIGIN      
        1 aaacaatgca tgggcggcaa ttcccctgac ctggtacagt gtaattgacg gacggtctga
       61 ccgcctgatt gccgatgcag tcagggagaa ccaggaagtg atgagatggt atggtgagag
      121 aaatattccc gttgaagtca atgaatcaca tcagtggagc ctgcgcgatg cccatgattc
      181 actggctgtg gcaatggctt tccttgctgc ctacaatgca aaaaatgccg gagtccggca
      241 ttatgtttca caatacatgt tcaacaatcc tcccgggact actgcggaaa tggatattgc
      301 aaaaatgctt gccaaggatg agatgatagc aaaactcagc ggcgattcat tcagggtttt
      361 ccggcaggtc agggcaggac tggcccattt ttctcctgat ccttcatttg cccagggcca
      421 gctggctgca tccgcagtta taagccttgc actgaagccg catattcttc atgttgtggg
      481 cttcagtgaa ggagatcacg ccatatttga tgatgagctt gtacaaagct gcaatattgc
      541 ccacggagtg cttcacaata ctctttacgg gatgcctgat atgacggaga ataaaaaagt
      601 agcagccagg aaaaatgaac ttatttccga agccggggtt ttacttgaag agctatataa
      661 caggggaaag aataaaagtc aggatccctg gtcagatccg ctcgtgattt cagatgcggt
      721 ccgggatggt cttcttgaca caccgcattt tcagggcaac ccgcatcttt gcggcaggat
      781 tgtcacaagg cttattgacg gggcctggta tgcaacagac agggatacag gggagaagat
      841 gtcggaatgg gacagaataa agaaactgaa gggaaagtaa acaaaaccgg agagcagggg
      901 taaaagaaaa aagcagtcac acgcatgtaa atcagagaaa gacagaaaaa acaggaaaac
      961 aaaatataca aagccggagc ttaaaagaag caaaacggca ataaaaacca gaaagcaggg
     1021 aaatatggcg ggaatagcag gaattgcagc ggacaatgca tcggaagaag tacttgaaat
     1081 gctttgtgtc atgcgtcaca ggggaaaaac acgtctttcc gtatttgaga atgagggcac
     1141 tactgccggg ataatctgga atgagccgga agatgattcc gtaaaagaat ttctggatgc
     1201 aggcgaggcg ggttattata acagtccggg taattatgca gttgtaagac ctgataacgg
     1261 aagtttcacc atgaggaggg atgaacttgg ttcagcaccc ctgtattatg gcagtgacaa
     1321 tgacggtaac atctgtttcg catcggaaat aaaggctctt cttcccataa ccagcaatat
     1381 cattgaactg tctcccgggc acgtttatga agatgacaag ctcagatcct actttggtat
     1441 acagacagga aagaaaacgg aaaaaagtcc ggacaggatt gcatcagagc ttagcggatt
     1501 gcttgataag gctgtttcgg actgtctgac aagtgaagac atcggatcat ttctttcagg
     1561 cggactggat tcgagtgtta tatgtgcatt ggcaaccagg cacctgaaaa agcttaaaac
     1621 ttttgctgcc ggattaaaag gggctccgga ccttgaacat gcacgcgaag tggcacggta
     1681 cttgcaaact gaccattacg aactgataat aacaccagaa aaactgacag caatactggc
     1741 tgaggtaatt tatcaccttg aatcatttga tgcacttctg gtaagatcat ccctgacgaa
     1801 ttatcttgtt gcccgtcttg cgtcagagca tgttgccgaa gtgttttcgg gtgaaggggg
     1861 ggatgaattg tttgccggtt acggatatct gaaatccatt ccgtttgaac atctggagaa
     1921 tgaactggta agaataacaa ataccatgca taacactgcg ctccagagag tcgacagaag
     1981 tgctgctgcc catagtctga ctcctcatat gatttttacc aatcccgagg ttgtcaagtt
     2041 tgcctttaca atcccggttg agtataagat acatggaaca accgaaaaat ggatactccg
     2101 gaaagccatg gaaggccggc ttccgtcagg gatactccgc aggccgaagg tgaaattctg
     2161 ggaaggagct ggagtaaagg aactgattgc cgtttatgca gaaaagaata tcagcgacaa
     2221 tgattacaga acagaaaggg atctgaaaaa tggcataatt ctcggttcaa aggaggaact
     2281 tctgtattac agaatattca gggagcattt cggatcagat attgacctgt cgtttatggg
     2341 aagaaccata acacactgag tttatgtgat ttccttctga ttatgagagc aggcgaagag
     2401 gatggttccg gtttcttcat agccatccag gaaggattcc cccacacgct tcaggatctg
     2461 agtgttgaat cctatcattg taagggctgc cagtcccgat ttcttattta tgatattttt
     2521 gagagtcatt ccgggttctt cctgaactat ttcaatgttt ttctctgtta tcggcagtct
     2581 tcccgtcctc acaagatcag tcagattaag cctggcttct tcttcctcgc cgtttttaca
     2641 ggtcccgaac agtttgatgt ttgattttct ccagtcggga tggcctgaaa taatgaaact
     2701 gagaaggata agcagatttg aattctccgg gtaatggaaa tccagccata catgtattcc
     2761 gttccggaaa atcatttcct ttctgctgga tgccagaagg catatatcaa agtctcctgc
     2821 tgctatcagg cctgtatttt caattatttc accaaggttc tccggatttt ccttgtcgta
     2881 ttcaaaaatc accatgttgt tttccattcc tgatatgccg ggtacctgta tagcctgggc
     2941 aagggccgag gtatatgacg gagatatcag tg
//
LOCUS       DAOSPX010000376         2955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_30311_length_2955_cov_4.591034, whole genome shotgun sequence.
ACCESSION   DAOSPX010000376 DAOSPX010000000
VERSION     DAOSPX010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30311_length_2955_cov_4.591034"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..>2955
                     /locus_tag="PLZ75_13360"
     CDS             139..>2955
                     /locus_tag="PLZ75_13360"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS73427.1"
                     /translation="MGRFFPLVFLTVFCACTSTGSRHTESNNYEAVRNGFARPGGTAR
                     PKVYWWCLNGNIDTLKAREEFIAMRDAGIGGFDMFEIGTRSRGIPAGPAFLGDESLEI
                     IKFVVNEAGKLGLEVGLNVASSWNAGGAWIRPEHGGKSLYRSVVVTQGNSSVQKVNIP
                     FPPIKFPESAIIGGTGKPMIPLRPDGKPEYYEEIAVLAIPAGSAALPDDSEILDISSF
                     FDPENDELSWEIPPGKWEIHRYVCSNSGQQLVLPSPHSAGLAIDHFDSSAVSTHFNYV
                     IGRLGTVLGDFRKTALKSLYLASYEARGFVWSSQLSSWFRQLHGYDIIRYLPVFFDNS
                     LFEEKTGARLQRDFRKTLSEMMINNLYKNAGNICNRHGLMINCEAGGPGYPLYNGPAE
                     PLKALGALDIPRGEFWVNHPRYYMDPNGTDSIDLMQVVKEVAAASHIYERGIVEEEAF
                     TSFEHWMEGPADLRPLGDRAFCEGMNRVVFHGFTHNPEGTGYPGIVYHAGTHMNPKRV
                     WWPKIKPFIDYITRLSYISQKADFFSDVLFYYGDKIPNAATPKNTHFKAGPGYDYEVI
                     NTEVLLDRLSMKNGKLRLSNGAVFRMLALENEPEINPDVLRKLEKLLQQGAVITGEKP
                     EKAAEVRNDPLSGESGTGLINKLWTEVADPQGFRPVKGKIHAGVRPAEMLSALNVAPD
                     LDYRDRETWLIDYIHYRSGKTDFYIVRNTSASWISRDCGFRQKNKTAEIWNPLNGEII
                     PVTVCRREGEHIRIPVTLPPFGSFIVVFREGGMKPPYTGVTVHGEEQDPPELEFSTKG
                     VLMMNRQNCELSGPQGSRTVEKSNDPETIDGPWLVYFDSKWGAPESVTFDRLISWTAH
                     SNPGIRYYSGTGRYRKTFSCDELNSASGDERIFLDLGEVSEVTEVWLNGEPLGISWTK
                     PFRFDVTEVIREGENDLEVEVANTW"
BASE COUNT          790 a          646 c          824 g          695 t
ORIGIN      
        1 ggctgaaccg tcactgggtg taatattctt ctcctggccg ctgttcgaaa gagacccgga
       61 aaggattgaa gcagtccggt tttaatttat ttcagattgt ttgtatataa atctgtcagt
      121 aattcaaaaa tcagagaaat gggcagattc tttcccctgg tgtttctcac cgttttctgc
      181 gcctgtacat caactggttc ccggcataca gaaagtaata attacgaggc tgtcagaaac
      241 ggttttgccc gtcccggggg aacggcccgt ccaaaagtat actggtggtg tctgaacggc
      301 aatattgata ccctgaaggc cagggaggag tttattgcca tgagggatgc aggtatagga
      361 ggtttcgaca tgtttgagat cggaacgaga agcaggggaa tacctgcggg acctgctttc
      421 cttggagatg aatcgctgga gatcataaaa ttcgtggtaa acgaagccgg gaagctgggg
      481 cttgaagtgg ggctgaatgt agccagcagc tggaatgcgg gaggagcctg gatcaggccg
      541 gaacacgggg gcaaatccct ttacaggtcg gttgttgtca cgcaggggaa ttcctcggtg
      601 cagaaagtca atattccctt tccgccgatc aaatttcccg aatccgctat aataggggga
      661 acggggaagc ctatgatacc attgaggcct gacgggaaac cggaatatta tgaagagata
      721 gccgttctgg caatcccggc gggttcagcc gctttgcccg atgactctga aattctggat
      781 atatcttctt ttttcgatcc ggaaaatgat gaactcagct gggagatacc gccgggaaaa
      841 tgggaaatac acagatacgt atgctccaat tccgggcagc agctggtgct tccgagtccg
      901 cattcggctg gactggcaat agatcacttc gattcctcgg cagtaagcac acattttaac
      961 tatgttatcg gaaggctcgg cacggtgctc ggggatttcc gcaaaaccgc ccttaaaagc
     1021 ctctatcttg caagctatga ggcaagggga tttgtatggt cgtcacagct ttcctcctgg
     1081 ttcagacaac ttcacggata tgacatcatc agatacctgc ctgttttctt tgacaacagc
     1141 ctttttgagg agaagacggg ggcaaggctg cagagggatt tcaggaagac tctctctgag
     1201 atgatgataa acaacctcta taaaaatgcc gggaatattt gcaacaggca tggtctcatg
     1261 atcaactgtg aggcaggcgg accgggatat cctctttaca acggacctgc cgaacccctt
     1321 aaggccctgg gagcactgga tattcctagg ggtgagttct gggtgaatca ccccaggtat
     1381 tacatggatc caaacgggac agacagcatt gacctgatgc aggtggtgaa ggaagtggct
     1441 gccgcatcgc atatttatga aagaggtata gttgaagaag aggcattcac ctctttcgag
     1501 cactggatgg aaggacccgc agacctgcgg ccactggggg acagggcttt ctgcgaaggg
     1561 atgaaccggg ttgtatttca cggattcaca cacaatccgg aggggacagg ctaccccgga
     1621 atcgtttatc atgcaggaac gcatatgaat cccaaaaggg tctggtggcc gaaaatcaaa
     1681 ccatttatag attatatcac ccgtctctca tatatttccc agaaggctga tttcttctcc
     1741 gatgtcctgt tctattacgg agacaagata ccaaatgccg ccacgcctaa gaatacccac
     1801 ttcaaggccg gaccgggtta cgattatgag gtgatcaaca ctgaagttct tctcgacagg
     1861 ctcagtatga aaaatggaaa gctccgcctt tcaaacgggg ctgttttcag aatgcttgca
     1921 ctggaaaatg agccggagat aaaccctgat gttctcagaa agctggaaaa actgctgcag
     1981 cagggggcag taataaccgg ggaaaagccg gaaaaagccg cggaggtaag gaatgatccg
     2041 ctgtcgggtg aaagcgggac agggcttatc aacaaacttt ggacggaagt tgctgacccg
     2101 caggggttca ggcctgttaa agggaagatc catgccggtg tcagacctgc agaaatgctt
     2161 tctgcgctta atgttgcccc ggatctggat taccgcgaca gggaaacatg gcttatagat
     2221 tatatccatt accggtccgg caaaactgat ttctacattg tcaggaacac ctccgccagc
     2281 tggatatcac gtgactgtgg tttccggcag aagaataaaa cagctgaaat atggaatccg
     2341 cttaacgggg aaataatccc tgtaactgta tgccgccggg agggtgaaca tataaggatc
     2401 cctgtcacac tgcctccttt cggatctttc attgtggttt tcagggaagg cggtatgaag
     2461 cctccttata ccggagtgac ggtccacgga gaggaacagg accctccgga actggaattc
     2521 agcacaaaag gtgttctgat gatgaataga caaaactgtg agctaagcgg accgcaggga
     2581 tcccggacag ttgagaaatc aaatgatccg gagacaattg acggcccctg gctggtgtat
     2641 tttgacagta aatggggagc accggaatca gtgacttttg acaggcttat ttcatggact
     2701 gctcacagta acccgggaat aaggtattat tccggtacgg gcagatatag aaaaacgttc
     2761 agctgtgatg aattgaattc agcatccggt gatgagagaa tattccttga tctgggggaa
     2821 gtatccgagg tgactgaggt gtggcttaac ggtgagccac tgggtataag ctggacgaag
     2881 cccttcaggt ttgatgtgac tgaagtgatc agggaggggg agaatgatct ggaagtggag
     2941 gtggcaaaca cctgg
//
LOCUS       DAOSPX010000377         2940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_30506_length_2940_cov_4.137955, whole genome shotgun sequence.
ACCESSION   DAOSPX010000377 DAOSPX010000000
VERSION     DAOSPX010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30506_length_2940_cov_4.137955"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLZ75_13365"
     CDS             complement(<1..405)
                     /locus_tag="PLZ75_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73428.1"
                     /translation="MKHLILKPGKTILLLTMMLVCGSAYSQQLSDYLMQAKAFSRTGN
                     PEASVRILTEALGKQRDYRLFLERGDANILIGKYPEALADYNAANSLVPLSGEYGLAK
                     VYALRGDAGTSVYHLELSMKSDFRKSEKEILLD"
     gene            complement(402..>2940)
                     /locus_tag="PLZ75_13370"
     CDS             complement(402..>2940)
                     /locus_tag="PLZ75_13370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOS73429.1"
                     /translation="GEITYVQISDLTYEITITTFTYTLSLADRPQLDVDWGDNTVSTA
                     DRIEKISLPNYYNRNVYKIRHTYPGPGIYKIVVQDPNRNYGVKNIPNSVNVVFSISTI
                     LFVDPAMGRNSTPVLLNPPYDKAALGHVFIHNPAAYDPDGDSLSYELTKCTREDGRVI
                     ENYTFPPATNYLRVDSVTGDLIWNTPADTGAYNVAMEIQEWRGGRKIGTVVRDMQIEV
                     FRTRNKPPVNGPLKNLCVEAGGIVDFTVSATDPNGDFIQQRANSGVFTIEKCPAAFTK
                     TDSAAGFSSSRFTWAPCYEAVRDQPYNIIFKAEDNNPEVKLADIDNMTIKVLGPAPVL
                     LNARPEGKIIRLIWENYGTDVISGFNIYRRNGPSTFVQDSCTSGLPVSTGFIRVGYIA
                     GHETVSYIDSNNGEGLEFGNEYTYRVVAVYKNGAESKASNEITSTLVSGLPVIKNVSV
                     TNTSPSAGSIFTGWKKPGRLDTIPAPGPWEYIIYRADGTTGTEFQMIRSIPTASLNDT
                     TFTDAPLNTQSQGYIYKIELWNRTPGNEFLIGEAAYASSVFLSVSPGDRKARFVIERN
                     VPWINTRYDFFRQNRTTMEFDSVGSTNQLTFTDLNLENEREYCYYVRSTGAYLMENMP
                     RNLINFSQISCVIPIDNEPPCPPDLTVTSQCDSLYNHLRWKIEDPECFADVAGYKVYY
                     ATTYEEHPSLIATIDDKNIFSYKHYPGEIVSGCYAVSAFDAAGNESAWSLKICVDSCN
                     FYEIPNVFTPNMDNINDRLIAKTSALVEKVDFKLFNRNGLLIFSTDKPRIDWDGTYKG
                     KIVSPGVYFYQCDVFERRITGLEQFHLSGFVHVITEQGAEIPRRETK"
BASE COUNT          710 a          716 c          675 g          839 t
ORIGIN      
        1 atccagcaat atttcctttt cgcttttcct gaaatccgat ttcatgctca gttccagatg
       61 atagaccgaa gtgcccgcgt cacccctgag tgcatatact ttcgccagac catattcacc
      121 tgagagtgga acaaggctgt tggcggcatt gtaatcggcc agggcttccg gatactttcc
      181 aatcaggata tttgcatcgc ctctttcaag aaacaggcgg taatcccgtt gtttgccgag
      241 tgcctccgtc agtatccgga cagaagcttc cggattcccc gtcctgctga aggcttttgc
      301 ctgcatcagg taatcggaaa gctgctgact gtatgctgat ccgcagacaa gcatcatcgt
      361 cagaagcaaa attgtttttc ccggtttcag tatcaagtgt ttcatttcgt ttccctcctt
      421 ggtatttctg ctccctgttc agtgatcaca tgaacaaatc ctgaaagatg aaattgttcc
      481 aggcctgtta tccggcgttc aaagacatca cactggtaaa aatacactcc cggggaaact
      541 attttcccct tgtatgttcc atcccagtca atccgcggtt tatcggtact gaaaatcaga
      601 agtccgttcc tgttgaacag tttgaaatcc accttctcca ccagggcaga tgtttttgct
      661 atcagcctgt cgttgatatt atccatattg ggcgtgaaca cattgggtat ttcatagaaa
      721 ttacaggaat cgacacagat cttcagagac caggcacttt catttccggc agcatcaaat
      781 gcagataccg cataacagcc tgatacaatt tcaccggggt agtgtttata gctgaaaatg
      841 tttttatcgt caatggtggc aatcagtgac ggatgctctt cataagtagt tgcataatat
      901 accttatagc cggcaacatc cgcaaaacat tccggatctt caatcttcca cctcaggtgg
      961 ttgtaaaggc tgtcgcactg gcttgttacg gtaagatccg gcgggcacgg cggttcattg
     1021 tcaatgggga taacacagct aatctgtgag aaattaatga gattccgggg catgttttcc
     1081 atcaggtaag ctcctgttga ccttacataa tagcaatact ccctttcatt ctcaagattc
     1141 aggtcagtga aggtcagctg attggttgaa ccaaccgaat caaattccat ggtggttctg
     1201 ttttgccgga agaagtcata gcgggtgttt atccacggaa cattcctttc tatgacaaac
     1261 ctcgcttttc tgtcgccggg acttaccgaa aggaatacgg atgaagcgta ggcagcctca
     1321 ccaataagaa attcgtttcc gggagtccgg ttccataatt cgatcttgta aatatatccc
     1381 tgcgactgtg tgttcagcgg agcatcagtg aatgtggtgt cattgagact ggctgtgggt
     1441 attgaacgga tcatctgaaa ttcggtgccg gtcgtcccgt cagcgcggta gattatatat
     1501 tcccagggac ccggtgccgg tatagtatcc agtcttccgg gttttttcca tcctgtgaaa
     1561 attgatcccg ccgaagggct ggtatttgtt acgcttacgt tctttatgac cggcagacct
     1621 gacacgagag ttgatgttat ctcattcgat gccttgcttt ccgcaccgtt tttataaacc
     1681 gcaaccaccc ggtaggtata ttcatttccg aattccaggc cttctccgtt gtttgaatca
     1741 atgtacgaaa cagtttcatg gccggctatg taaccgaccc tgataaagcc cgttgaaacc
     1801 ggcaatcccg aagtgcatga atcctgaaca aaggtggagg gaccgtttcg cctgtaaata
     1861 ttgaatccgg aaatcacatc cgtgccgtaa ttttcccaga taagcctgat tatcttgcct
     1921 tcgggacggg cattcaggag aacgggagcc ggaccaagta ccttgatcgt catattgtca
     1981 atatccgcga gctttacttc aggattgttg tcctcagcct tgaatatgat attatagggc
     2041 tggtccctga ctgcctcata gcaaggcgcc catgtgaatc tcgatgagga gaatcccgct
     2101 gctgaatcag tttttgtaaa tgctgcagga catttttcaa ttgtgaatac tcctgaattg
     2161 gctctctgct gaataaaatc cccgttggga tcagtggctg atacggtgaa atccactatt
     2221 cctcccgcct ccacacagag atttttcaga ggaccgttta ccggcggctt attccgggtc
     2281 ctgaaaacct ctatctgcat atcacggacc acggtgccga ttttccttcc tcccctccat
     2341 tcctgtatct ccattgccac attataggca ccggtatcgg caggtgtgtt ccatataaga
     2401 tctcccgtca ccgaatcaac cctgaggtaa tttgttgccg gggggaatgt gtagttttca
     2461 ataacccgtc catcctcacg cgtgcacttt gtcagctcat acgaaagact gtccccgtca
     2521 ggatcatagg cggcaggatt gtgaatgaag acatgaccga gggctgcctt gtcataggga
     2581 ggattaagca ggaccggagt gctgtttctt cccatggcgg gatcaacaaa cagaatcgtt
     2641 gagatggaga aaacaacatt cacgctgttg ggtatgttct ttaccccgta attcctgttc
     2701 ggatcctgta caactatctt gtatataccc ggccccggat aggtgtgccg gatcttgtag
     2761 acattcctgt tatagtaatt tggaagggat atcttttcaa tcctgtcagc cgttgaaacc
     2821 gtattgtctc cccagtccac atcaagctgg ggcctgtcgg ccagactcag ggtataggta
     2881 aacgtggtga tggttatttc ataggtaaga tcggaaatct gaacatatgt gatctctcct
//
LOCUS       DAOSPX010000378         2936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_30568_length_2936_cov_3.816730, whole genome shotgun sequence.
ACCESSION   DAOSPX010000378 DAOSPX010000000
VERSION     DAOSPX010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30568_length_2936_cov_3.816730"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLZ75_13375"
     CDS             complement(<1..759)
                     /locus_tag="PLZ75_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOS73430.1"
                     /translation="MKVLKFGGSSLGTPERIKRVKEIIRRNSLPCIVVVSAFEGVTDQ
                     LSQIAELASQGREEYSGNLEKLISAQREFASGLLSDEDDLNRAQKHIDAISGELTEVL
                     RGLYLLRESTAHALDLIMAGGERISSFIISLYLEDSLYIDSRELIKTEGRPGNAIVDF
                     PVTNSLIRSALENSDRLAVLPGYIASNHQGGTTTLGRGGSDYTAAIVAAALKSEILEI
                     WKDVDGFMTADPKKVEKAYTIENLTYSEAMELSNF"
     gene            complement(840..2696)
                     /locus_tag="PLZ75_13380"
     CDS             complement(840..2696)
                     /locus_tag="PLZ75_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS73431.1"
                     /translation="MLKNHPRGLLVAFFSNMGERFGFYTMMAILVLFLQAKYDLPAKA
                     AGDIYSWFYFAIYALALIGGVLADSTKKYKLVILAGIIVMFIGYFVMAIPGMTLTVTL
                     IGLGTIALGNGLFKGNLQAVVGQMYDDPRYSKLRDTAFMIFYMGINIGAFFAPHAADG
                     MRNWWLKTNGFLHDGSLPSLCHQFNKGTLTDITVFQQLADKVSLSGPVSDLSAFANNY
                     LEVFSRGYNYAFAVAAGAMIISLLVYVIFNRLLPSKDKVVSAVRAEEKNIKTAEKIEF
                     EALPIAAAIAAIGVTAFLLQLLNQIDVATGLAFGVFAGFVTWIILSSRKEERARVTAL
                     VLVFFVAIFFWMSFHQNGLTLTLFARDYTVKQVGPFTGLFFTLPSILAFIAVIAGIVM
                     LFYKKQPRNFRITGAVLLAAGLWFVVFFLTGFDPTTLLGRFLSPFGPQNAISPEIFQQ
                     FNPLFIVALTFPVLAFFSWLNKKGIEPSTPKKIGIGLLIASVGFLIVLIGSIGAPSPA
                     SLGGSPVADADRVSPYWLINSYLILTVAELFLSPMGLSFVSKVAPSRFQGLMQGGWLL
                     ATAAGNKLLFVGTLLWDKIQLSTLWLIFIVCLLLSAGFIFSILKRLEKASGS"
BASE COUNT          822 a          684 c          682 g          748 t
ORIGIN      
        1 gaagttggag agttccatgg cttcggagta tgtcaggttc tcaattgtat aggctttctc
       61 cacttttttg ggatcggctg tcataaatcc gtccacatcc ttccagattt cgaggatttc
      121 cgactttaat gcagcagcca caattgctgc cgtatagtcc gagcctcccc gcccgagtgt
      181 tgtcgtgccg ccctgatggt tgctggctat ataacccggc agcactgcaa gcctgtcaga
      241 attttcaagt gctgatctga taagggaatt ggtgaccgga aaatcaacaa ttgcatttcc
      301 tggcctgcct tccgttttga tcagctccct ggaatcaata taaagcgaat cctccaggta
      361 caggctgatg ataaaggagg atatcctttc accgcctgcc attatcagat ccagggcatg
      421 agcggttgat tcgcgcagga gatagagtcc ccgcagtacc tctgtgagtt caccggaaat
      481 agcatcaatg tgtttttgtg ccctgttaag atcatcctca tcgctcagaa gtccggatgc
      541 aaattcccgc tgggctgaaa tcagtttttc aagatttccg gaatactctt ccctgccctg
      601 cgatgcaagt tccgctatct gcgacaactg gtcggtcact ccctcaaaag ccgaaacaac
      661 aactatgcac ggaagactgt tccgccttat gatttcctta acccttttta tcctttccgg
      721 tgtccccagt gatgaaccgc cgaatttcag gaccttcata tttcttccat ttttatatcg
      781 taaaagggcg ttgtattaac taacgccctg tataaatccg ttcaccaaag catcatgatc
      841 taacttcctg aagctttttc aagtcttttc aggatagaga agatgaatcc tgctgaaagt
      901 aacaggcaga caataaatat cagccataag gtgctcagct ggatcttgtc ccacaggagt
      961 gttccgacaa aaagaagctt gttgccagcg gccgtggcaa ggagccatcc tccctgcatc
     1021 aggccctgaa accttgaggg agctactttt gatacgaagg aaagtcccat cggactgagg
     1081 aagagttctg ccacagtaag aatcagataa ctgtttatca gccagtatgg cgaaaccctg
     1141 tcggcatccg caacaggcga cccgcccagt gatgcgggag agggtgctcc tatggaacct
     1201 atcagtacaa taaggaaacc tactgaagca atcagtaatc ctatgcctat ctttttgggt
     1261 gtggatggtt caataccttt tttgttgagc catgaaaaga atgcaagcac ggggaaggta
     1321 agcgctacaa tgaacagagg attgaactgc tggaaaattt ccggcgatat ggcattctgc
     1381 ggtccaaagg gggataagaa ccttccgagc agggtggtgg gatcaaatcc tgtaaggaaa
     1441 aagacaacga accagaggcc ggcagcaagc aaaacagccc cggtgatcct gaaattccgc
     1501 ggctgttttt tgtagaacag cataactatc cccgcaataa cggcaataaa agcaagtatt
     1561 gagggaagtg tgaaaaacag accggtaaag gggccaacct gcttgaccgt ataatccctt
     1621 gcaaaaagtg taagtgtaag cccgttctgg tgaaatgaca tccagaagaa aatagcaaca
     1681 aagaagacaa gtaccagtgc ggtcactctt gccctttctt cctttcgtga actgaggatt
     1741 atccatgtga caaagcctgc aaagacacca aatgccagtc cggttgcaac atcaatctga
     1801 ttcagcagct gaagcaggaa ggcagtgact cctatggctg cgattgctgc tgctatggga
     1861 agagcttcga actctatctt ttcagctgtt ttgatatttt tctcctctgc cctgactgct
     1921 gatacaacct tgtccttgct gggcagcagt ctgttgaaaa tcacatatac cagcagcgaa
     1981 attatcattg ccccggctgc cacggcaaag gcataattgt aacccctgga aaacacctcg
     2041 agataattat tggcaaaagc tgaaagatcg ctgaccgggc cactgagact gaccttatcg
     2101 gccagctgct gaaatacggt aatatcggtc agtgtgccct tattgaactg atgacagagg
     2161 gaggggagac tgccgtcatg cagaaagcca ttggttttaa gccaccagtt gcgcatgccg
     2221 tcggctgcat ggggtgcaaa gaaggcaccg atattgatac ccatgtagaa gatcataaag
     2281 gctgtatccc ttagttttga atatctggga tcatcataca tctggccgac taccgcctga
     2341 aggtttccct tgaacaaacc gttgcccagt gcaatggttc cgagaccgat aagggtaacc
     2401 gtaagagtca tgccgggtat ggccattacg aaataaccta tgaacatcac tattattccg
     2461 gcaagtatca caagcttgta tttcttggtg gaatctgcca gtacacctcc tataagggca
     2521 agtgcataaa tggcaaaata gaaccagctg taaatgtcgc ccgctgcctt ggcagggagg
     2581 tcatatttag cctgaagaaa aagtaccaga atggccatca tggtatagaa gccaaacctt
     2641 tcacccatgt ttgagaaaaa ggcaacgagc aatcccctgg gatgattttt tagcataaat
     2701 tcagttttaa ttcatttaca atttgttgct gacctgaagg ttaaaaatac aatcagccgg
     2761 aaaaccggta ctgccggtcc gttaccggaa ggaaacaaat atattaatct tttttaatta
     2821 ttgcttttag agattaaatt tgtggcaaaa tgcatttaag gatgaagata ttcagctccg
     2881 cgcagatcag ggagattgat gattatacaa taagacagga acctgttgct tctgtt
//
LOCUS       DAOSPX010000379         2916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_30851_length_2916_cov_3.184551, whole genome shotgun sequence.
ACCESSION   DAOSPX010000379 DAOSPX010000000
VERSION     DAOSPX010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30851_length_2916_cov_3.184551"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLZ75_13385"
     CDS             complement(<1..858)
                     /locus_tag="PLZ75_13385"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOS73432.1"
                     /translation="MALRCGITGIAGVGKTTIFNCISNTKAATGMNVPGGAKTNIGMV
                     RVPDPRLYELEKLQPSEKITPTTVEIVDIPGLSGSPAGAEGTGNRFLGDIRNTDALIH
                     VLRCFDDEMLPHVEGSVDPVRDMETVNLELQVRDHESLGKKTEKLRKTAKSGDKDAIR
                     GMEVLERYMAHLGEFNDARTLDAGSDAGRYIADLSLLTMKPVLYVCNVDEKSALSGNA
                     YVDRVRKHLTGQDAEILIIAGAIEAEISELENAADRNAFLKDIGLPEPGVDRLVRAAY
                     KLLDLRTFFT"
     gene            complement(866..2227)
                     /gene="hisS"
                     /locus_tag="PLZ75_13390"
     CDS             complement(866..2227)
                     /gene="hisS"
                     /locus_tag="PLZ75_13390"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOS73433.1"
                     /translation="MSKPSIPKGTRDFQADEMLRREFIFETIRSVFRLYGYQPIETPA
                     MEHLSTLLGKYGEEGDRLIFRILNSGDFLSGVSDRELLEREPGRLSSKICEKGLRYDL
                     TVPFARFVVQHRNKLVFPFRRYQIQPVWRADRPQKGRYREFFQCDADVIGSDSLLNEY
                     ELVRIVEDVFSRLDLKVAVNINNRKILSGFAEYIGEEDRLTDITVAIDKLEKTGYDAV
                     YAELREKGISAEAINKLKPVLRLQGDTVRKLNELEILLAGSDSGMQGIAEMKTLFSMI
                     EGSGLRSRIVFDLSLARGLNYYTGTIIEVKSEDMAMGSICGGGRYDNLTGIFGLEGVS
                     GVGISFGADRIYDVMLQLGLFEKRIPASTRVLVINFGEKEQPYALEIANRLRNSGIST
                     ELYPDPVRLGKQFTYAGSRGIPYVIIAGEEEIGKGECTLNVMETGEQKRLALEDIGSH
                     ITA"
     gene            complement(2310..2666)
                     /locus_tag="PLZ75_13395"
     CDS             complement(2310..2666)
                     /locus_tag="PLZ75_13395"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOS73434.1"
                     /translation="MEEFGVPPESERNWAMMCHLSSFAGYFFPFGGIIGPLICWLSKK
                     DESPWVYENGRASLNFQISILLYMVLALPLCLIIVGIPILMFLAALELICVIIASVRA
                     AKGERYRYPLSIPFIQ"
     gene            complement(2715..>2916)
                     /locus_tag="PLZ75_13400"
     CDS             complement(2715..>2916)
                     /locus_tag="PLZ75_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73435.1"
                     /translation="AIEEKSPETVVLHKVGPSGDADGGYCFKMLIGTDDAVMYYYNSH
                     RIDGQNPDGLLPSDLKRLARFR"
BASE COUNT          722 a          794 c          606 g          794 t
ORIGIN      
        1 ggtgaaaaaa gtccggagat caagcagctt ataggcggcc ctcacgagcc tgtcaacacc
       61 cggttccggc agacctatgt ctttcaggaa ggcattgcgg tcagcggcat tctccagttc
      121 ggaaatctcg gcttcaatgg caccggcaat aatgagaatc tctgcgtcct gacccgtcag
      181 atgctttctc accctgtcaa cataggcatt tcctgaaagc gcggattttt catcaacatt
      241 gcatacatac agcacaggct tcatcgtaag aagtgaaagg tcggcgatat acctgcccgc
      301 gtcacttccg gcatccagtg tccgtgcatc attgaattcc ccgagatgtg ccatgtatcg
      361 ttccagtacc tccatccccc ttatcgcatc cttgtctccc gattttgccg ttttcctcag
      421 tttctcggtt tttttcccca gcgattcatg atcccttacc tggagctcaa ggtttaccgt
      481 ttccatgtcc ctgaccggat caactgatcc ttcaacatgt ggaagcatct catcatcaaa
      541 gcatctcagc acatggatca gtgcatcggt atttcggatg tcgccaagga acctgtttcc
      601 cgttccctca gcaccggcag ggcttcccga caaaccgggt atatccacta tctccacggt
      661 ggttggtgtg atcttttccg atggctgaag cttttccagt tcatagagcc tcgggtcggg
      721 cacccgtacc atcccgatat tggtttttgc acctcccgga acattcattc ccgtagctgc
      781 tttggtattt gatatacagt tgaatattgt tgtcttgcca acaccggcaa ttcctgttat
      841 cccgcaacgc agagccatga tatgcctaag ctgttatatg tgatccgata tcttcaagcg
      901 ccagtctttt ctgctcacct gtttccatca cattgagggt gcattctcct tttccgatct
      961 cttcctcacc ggcaataatg acatagggta tcccccgtga accggcatat gtaaactgct
     1021 tcccaagtct gacaggatca ggataaagtt ccgtacttat tccggaattc ctgagcctgt
     1081 tagcaatctc cagggcgtag ggctgttcct tttcaccgaa gtttatcacc agaacccggg
     1141 ttgaggcagg aattctcttt tcaaagaggc ctagctgcag catgacatca taaatcctgt
     1201 cggcaccgaa ggaaataccc acccccgaca ccccttcaag tccgaaaata cccgtcagat
     1261 tatcatatct cccgccgccg catatgctgc ccattgccat atcttccgat ttcacttcaa
     1321 tgattgtgcc ggtatagtaa ttaagtcccc tggcaaggct gagatcaaat acaatcctgc
     1381 ttcggagtcc cgatccttca atcattgaga acagtgtttt catttcggca atgccctgca
     1441 ttccggaatc cgaaccggca agcagtattt ccagctcatt cagtttcctg acggtatctc
     1501 cctgcagcct gagaacaggt ttcagcttgt ttatggcctc cgccgatatg cccttttccc
     1561 tcagctcggc atatactgca tcataacccg tcttttcaag cttgtcgatt gccactgtaa
     1621 tatccgtcag ccggtcttcc tcaccgatat attctgcaaa tccggatagt attttgcggt
     1681 tgtttatatt gactgcaacc ttcagatcaa gcctggaaaa aacatcttca acgattctta
     1741 ccagttcgta ttcattaagt aatgagtcgc ttcctataac atcagcatca cactggaaaa
     1801 attcccggta tcttcccttc tggggccggt cagctctcca cacaggctgt atctggtatc
     1861 tcctgaaggg aaaaaccagt ttattccggt gctgcactac aaagcgtgca aagggaaccg
     1921 tcagatcata cctcagtcct ttttcacaga ttttcgaaga aagtcttccg ggctcacgct
     1981 caagcagttc cctgtcactt acccctgaaa gaaaatctcc cgaattaagt atcctgaaaa
     2041 taagcctgtc cccttcctca ccatatttcc ccagaagggt tgaaagatgt tccattgcag
     2101 gcgtttcaat gggctgatat ccgtacagtc tgaataccga cctgatggtt tcaaagatga
     2161 actccctcct gagcatttca tctgcctgga aatcccgtgt ccctttggga attgatggtt
     2221 ttgacataaa tgaaaaagca aattattcaa gtgaaagcac aaaagtaaga aagatatgcg
     2281 atatcaggga attttccgga aacgacatcc tactgaatga acgggatgga gagcggatac
     2341 ctgtaccttt cgccctttgc ggccctgacg gatgctatga tcacacagat cagttcaagt
     2401 gcggccagga acatcaggat gggtattcct acaattataa ggcagagcgg aagcgcaagc
     2461 accatgtaca gaagaataga tatctggaag ttcagggatg ccctgccgtt ttcatacacc
     2521 cacggcgact cgtccttttt ggacaaccag cagataagcg gtcctataat acctccaaag
     2581 gggaaaaaat aacctgcaaa ggatgacaga tggcacatca tggcccagtt tctttcagat
     2641 tccggcggaa ctccaaattc ttccatttca aatactgttt atgtaaaaga cggataaatt
     2701 cttcctgtga tgcatcacct gaatctggca agccgtttaa ggtctgaagg aagtaatccg
     2761 tcgggattct gcccgtcaat cctgtgggaa ttataataat acatcactgc gtcatccgtc
     2821 ccgataagca ttttgaaaca gtatcctccg tctgcatcgc cagaaggccc caccttgtga
     2881 agcacaacgg tttccgggct tttctcctca atggcc
//
LOCUS       DAOSPX010000380         2900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_31084_length_2900_cov_4.724077, whole genome shotgun sequence.
ACCESSION   DAOSPX010000380 DAOSPX010000000
VERSION     DAOSPX010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31084_length_2900_cov_4.724077"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="PLZ75_13405"
     CDS             complement(<1..1252)
                     /locus_tag="PLZ75_13405"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOS73436.1"
                     /translation="MKKNKSVGNRFFSIPGKLALTLHTVVFLIFSGTLQIHAADTYSQ
                     GTDVVPDFSDAVAIKDGGLYVSPQQQTVSGKVRDETGNPLIGVSVVVTGTTVGTITDI
                     DGNYSLDVQSGSAVLSFSFVGMESQTVEVGGRSVINITMREAAIGLDEVVVTALGISK
                     AKREIAYSSQDVKSDDFIQAREINIGSALTGKIAGIDATQINAGPGSSSRVVIRGNNS
                     LSNVDGINQQPLYVINGLPMNNKSNAASQNTTGLNVDRGDGIGMINPDDIETISVLKG
                     GAAAALYGSQAANGVILITTKSGQGQQGIGIELNSVANFGTPSIYPVYQYEYGQGQNG
                     RKPMTQAEAQASGRLSFGSKMDGSDYTAVDGKTYPYSPVHVKDNIKNFYRPSSDITNT
                     VALTAGGAQSNLRFSVSDVRANSQT"
     gene            complement(1755..2483)
                     /locus_tag="PLZ75_13410"
     CDS             complement(1755..2483)
                     /locus_tag="PLZ75_13410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS73437.1"
                     /translation="MKDLSNKIALVTGASSGFGAGIAKHLSRAGAKVYIVARNAGKLE
                     SVAKETAASPIVANITDPNDWDKVFEIIIKENNHLDILINNAGSGGIIKPINEQDDTN
                     IIHTIQTNLTGAILGSKRAAAVMKKQHSGTIVNISSVCSFEAWPGWSVYGAAKAGLEQ
                     FTRHLYVEMRPYGIKATILVPSWGATNFNINANIEPFTTDVISKIIQPDDIGQVVANI
                     CRLPDHLIIPEMILLPMTQEIIPY"
BASE COUNT          822 a          615 c          565 g          898 t
ORIGIN      
        1 gtgtctggct gtttgccctg acgtctgata cggaaaatct caggttggac tgagctccgc
       61 ctgcagtaag ggcgacagtg ttggtgatat ctgaactggg tctgtaaaag ttctttatat
      121 tatcctttac gtgaaccggt gaatagggat aagtttttcc atctacagca gtataatccg
      181 atccgtccat ttttgacccg aatgacaaac gacccgaagc ctgtgcttca gcctgtgtca
      241 tcggctttct tccattctgt ccctgaccgt attcatactg ataaacaggg tatatgctgg
      301 gtgttccgaa atttgcaact gaattgagtt ctattcctat tccctgttgt ccctgtccgg
      361 attttgttgt tataagaatt acaccattgg ccgcctggct tccgtacaaa gctgcagctg
      421 caccaccctt gagaacggag atggtttcaa tatcatccgg gttgatcatt ccgataccgt
      481 caccgcggtc aacattaagt cctgtggtat tctggctcgc agcattgctt ttattgttca
      541 tgggcaagcc gtttattaca tacaggggct gctggttgat gccatcgaca ttgctcaggg
      601 agttatttcc cctgatgacc acgcggcttg aactgcccgg accggcattg atctgtgtgg
      661 catcaattcc ggctattttt cccgtaagag ctgagccaat attaatttcc cgtgcctgaa
      721 tgaaatcatc actttttaca tcctgtgatg aataggcaat ttcacgtttg gcctttgata
      781 tgccaagggc agtgacaacc acttcatcca gacctattgc tgcctctctc attgtaatat
      841 ttattactga acggcctccg acctcgactg tttgtgattc cattcccaca aatgaaaaac
      901 tgaggacagc actgcctgat tgtacatcca ggctgtaatt accgtcaata tcagtgattg
      961 tacctactgt tgtgcccgta accaccaccg acactccaat caggggattg ccggtttcat
     1021 cccgtacttt tccgcttact gtctgctgct ggggacttac atacagtccc ccgtctttaa
     1081 tcgcaactgc atcagaaaaa tccggtacaa catcggttcc ttgagaataa gtgtcagctg
     1141 catgaatctg taatgttcct gagaatataa gaaatacaac agtatgcaga gtgagggcaa
     1201 gttttcccgg aatcgaaaag aacctgttcc ccactgattt gttttttttc ataaatcgtt
     1261 ctgtttagat ttaacaatta aaataatgta ctaccaaatg agttttaact agttaaaatg
     1321 ttgctgagta attttcccgg gtgttttatg aaagcctcat gcatttaatg gccaaattag
     1381 gtgaaaaata ttttcaatta caaaatgaat ccggccagag gattattttt taaaatattt
     1441 aacatttata tatcacaaca gctaaattgt aatgagaccg tccctgaaaa taaaagacag
     1501 tgtcattata gatttatgta ccggcagccg gattggtgag tgaaatacaa tttcaaattc
     1561 tgaaacagag ccttgaattg ctgacagtca gccacagcgc agggatcagt gctcaaatca
     1621 aagaaaagaa tgctccatac actatgctcg ggaaaaagag aaaacgcgtt cgcagctgtc
     1681 attacaggca gcttcagggc taatcagttt tcacagaaga ggtaattatg cgtttacagc
     1741 atatccaaac cctctcagta tggaataatc tcttgtgtca taggtagtaa aatcatctcc
     1801 gggattataa gatgatcagg taacctgcag atatttgcta ctacttgacc gatatcatca
     1861 ggttgaatta ttttactgat tacatcagtt gtaaagggtt caatatttgc attaatgtta
     1921 aaattcgttg ctccccatga tggtaccagg atagttgctt ttattccgta aggccgcatc
     1981 tccacataca agtgacgtgt aaattgttca agtccggcct tggctgctcc gtaaacactc
     2041 cagcctggcc atgcttcgaa tgaacaaact gaagaaatgt tgacaatggt cccggaatgc
     2101 tgttttttca taacagctgc ggcccttttg cttcctaata tggccccggt aagattggtc
     2161 tgaattgtgt ggattatatt tgtatcatcc tgttcattaa tgggtttaat aataccacca
     2221 gaacctgcat tattaataag aatatccaaa tgattatttt cttttataat aatttcaaat
     2281 actttatccc agtcattggg atcagtaata tttgccacta ttggggaagc agctgtttct
     2341 ttggcaaccg actccaactt gccggcatta cgagcaacaa tgtaaacctt ggcaccagcc
     2401 ctgctcaaat gcttcgcaat tcctgccccg aaaccactgg aagcacctgt aaccagggca
     2461 attttatttg acaaatcttt cattttaaga atatttttaa atagttcgtt aaagtataat
     2521 ataagtattt gaatgtcaat aaaatagtta ataaataatg gcggaataat ttggttaagt
     2581 ccctgacgat cagtaactta atagattaac caaatctaga ccaagttatg atcatcaggg
     2641 aacataaacc ttataatgca cttatttcca gcatgataga caaattgcca aatattaata
     2701 ggtggagaaa agcatttctt tcagagattt tcatcctgtt tttgtctata cgtggaagaa
     2761 ttaatttctt acagcttgca agatatggaa aatataaaga acagcgatat cgccagcagt
     2821 ttgaaaaacg ttttgatttt ctaacctttc tcctataaat ccatcccaaa ttatagcgcc
     2881 tttggggttg cgagtttctc
//
LOCUS       DAOSPX010000381         2879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_31380_length_2879_cov_3.165722, whole genome shotgun sequence.
ACCESSION   DAOSPX010000381 DAOSPX010000000
VERSION     DAOSPX010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31380_length_2879_cov_3.165722"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLZ75_13415"
     CDS             complement(<1..204)
                     /locus_tag="PLZ75_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73438.1"
                     /translation="MSYQCVVRGAGGELITEKSIGVKTTISRGSPFQIIVYEETYSPN
                     PSTNENGLLTLKIGSGVTSGDFSK"
     gene            complement(283..771)
                     /locus_tag="PLZ75_13420"
     CDS             complement(283..771)
                     /locus_tag="PLZ75_13420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS73439.1"
                     /translation="MRQIKRIISFLSLILFYTQISTGQESIPATGGTATGAGGRASYT
                     VGQVVYHQFKGSTGYIIQGVQQPWEISIVTAIENTEDITLDCMVYPNPVKGCLKLVIG
                     SFENDNMSFQLITMSGILIQDFKIEDKETILLMDNLRPGIYFLKVIKNNLQVKVFKIV
                     KQ"
     gene            complement(823..1425)
                     /locus_tag="PLZ75_13425"
     CDS             complement(823..1425)
                     /locus_tag="PLZ75_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73440.1"
                     /translation="MKKLIVLTVSLFIILPKLAGQEITLKPYGIKSGIIEYSYSGDKV
                     GKGTLYFDDYGMKSAMYTDAVADGEKGKGWIVTFGDYQYIWDLDHPDEGLKLENPIIT
                     WLAESSKGDFESFTESMFSKMGFNKAPDETFLGKPCKVVKGDMGKVLTWNGILMLLDM
                     NVMGTVSHQEATKIQTNVPVDAKYFAIPKNIKFSEMPMMF"
     gene            complement(1500..>2879)
                     /locus_tag="PLZ75_13430"
     CDS             complement(1500..>2879)
                     /locus_tag="PLZ75_13430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS73441.1"
                     /translation="KKARNANEKYISVLDHLPGKLPYIIPYHLAREGVKVVSEKGREC
                     ANSLDKLHNYLKELCTRDAYNEEEKNKRAVSSSTPEDKYGPAGFGMEFGNGYIGKLDL
                     FEYRIDYWNKEDATAPAAIVYIRDTIDTNFNLKTLRFTEIGFLKWKMNLDGGQYFDIN
                     IDCRPEMPYVVNVKGSVDYRSREVFWVHTTLDPFTRELPDNPMAGYLPPIDSTGYQAG
                     WVNFTIEPEARLPHGTSFENQAFVNFDGVGPWGPAPEYGPYTNIFDFIPPRSYVEQLE
                     PVQTSLSFNINLNGDDTGSGIDHFDIYVSKDYGDAYLWKTTKDTTVTFTGENGSYYGF
                     WSIATDRVGNTETMKSTPDTYTFIESSQTGIDQSETIDVISRIRIYPNPFRESAIIGY
                     ELSQPTHVKIEVYNLSGHKVRTMINENKSPGYNEFEFHAESLPDGMYFIHIMSGEYRS
                     AGKLILLRP"
BASE COUNT          786 a          630 c          550 g          913 t
ORIGIN      
        1 tttcgagaaa tccccggagg taacaccgct tccgatctta agagttagta gcccgttttc
       61 atttgtggaa ggattgggag aataagtctc ctcgtaaaca attatctgga aaggacttcc
      121 ccgagaaata gtggttttta ctcctatgct cttttcggtg ataagttcac cgcctgcacc
      181 tcttaccaca cattgataac tcatcaattg cggcgcttgg gcaataatat tagcagacaa
      241 gacagcaatt gctgtaagag ttaataattt tttcataatg atttactgtt taacgatttt
      301 aaaaacttta acctgaagat tatttttaat gactttcagg aaatagatcc ctggcctgag
      361 attatccatt aataaaatgg tctctttatc ttcaatctta aagtcttgaa ttagtattcc
      421 actcatagta ataagctgga aactcatatt gtcattctca aacgatccga taaccagttt
      481 gagacaacct ttgacaggat tgggatatac catacaatcc agagttatat cctctgtatt
      541 ttcaattgcc gtaacaattg agatctccca gggttgttga acaccctgta ttatataccc
      601 ggttgatccc ttaaattgat ggtaaactac ctgaccgacc gtataggagg cacggcctcc
      661 cgcaccggtg gctgtgccgc cggttgcggg aatggattcc tgacctgttg aaatctgagt
      721 atagaataag atcagggaga ggaaagaaat gattctttta atttgtctca tagatttcga
      781 attattattt attcttaatt ccctttcagg aaatcattgt ttctaaaaca tcatcggcat
      841 ttcggagaac ttgatattct ttggaatagc aaaatatttg gcatcaacgg gaacattggt
      901 ctggatcttt gtcgcctcct gatgggacac cgttcccatt acattcatat ccagaagcat
      961 gagtataccg ttccatgtaa ggactttccc catatcccct tttactacct tacatggttt
     1021 accaaggaaa gtttcatcag gggccttgtt gaatcccatc ttagaaaaca tcgattccgt
     1081 gaaggattca aaatctcctt ttgatgattc ggccagccat gtgattatcg ggttttcgag
     1141 ctttaatccc tcatcaggat gatcaagatc ccatatatac tggtagtccc cgaaagtaac
     1201 aatccagcct ttaccttttt caccatctgc aaccgcatcg gtatacatgg cgcttttcat
     1261 accataatcg tcaaaataaa gtgttccttt tccgacttta tcaccagaat aggaatattc
     1321 aataatccct gatttaatac cgtaaggttt cagggtaatt tcctgtcctg ccagcttcgg
     1381 caggataata aacagactga ctgttaatac aattagtttt ttcatgatca taattattgg
     1441 aatgtttcta ttgttttaat aattgaagaa tgaaccataa tatgtatata tttagcattt
     1501 tacggtctta acagtatcag tttcccggca gacctatatt cacctgacat aatatgaatg
     1561 aaatacatcc cgtcggggag cgactctgca tgaaattcaa attcattata tcccggagac
     1621 ttgttttcat tgatcattgt cctgacttta tgacctgaca ggttatatac ctcgatcttc
     1681 acatgtgttg gttgtgacag ctcataaccg atgatggctg attcgcggaa tggattggga
     1741 tagatcctga tccggctgat tacatcaata gtctcagact gatctatccc tgtctggctc
     1801 gattcaataa aagtatatgt atccggtgta cttttcattg tttcagtatt tccaaccctg
     1861 tcggttgcaa tggaccagaa accatagtac gaaccatttt caccggtgaa tgtcacggtc
     1921 gtatctttag tcgttttcca taaataggca tctccatagt cctttgaaac ataaatgtcg
     1981 aaatgatcga ttccgctccc ggtatcatct ccgttaagat taatattgaa tgacaggctg
     2041 gtttgaactg gttcaagttg ttcaacataa ctccggggag ggataaagtc gaaaatattg
     2101 gtgtaaggcc catattccgg tgcaggcccc caagggccga caccgtcgaa attcacaaat
     2161 gcctggttct cgaagctggt tccatggggt aaccgggctt cgggttctat ggtgaaattc
     2221 acccagcctg cctgataccc ggttgaatca atgggcggaa gatatcctgc cataggatta
     2281 tcgggtaatt cccttgtgaa cggatcaagt gtggtatgaa cccagaacac ttccctgctc
     2341 cggtaatcaa cgcttccttt tacattcaca acatacggca tttcaggccg gcaatcgata
     2401 ttgatgtcaa aatactgtcc tccgtcgagg ttcattttcc atttcaggaa acctatttcg
     2461 gtaaacctga gtgttttcag gttgaaattg gtatcaatag tatcccttat ataaacgatc
     2521 gcagccggag cagtggcatc ttccttattc cagtaatcga tacggtattc aaacagatcg
     2581 agtttaccaa tatatccatt gccaaattcc attccaaagc ctgccggacc atatttatcc
     2641 tccggtgtac tcgacgaaac tgctctctta tttttttctt cctcgttata agcatccctt
     2701 gtacaaagtt ccttcaggta attgtgaagc ttgtccaggc tgttggcgca ttctcttcct
     2761 ttctccgaaa caacttttac accttccctg gctaaatggt atggaataat ataaggcagc
     2821 ttaccaggga gatgatctaa aacgctgata tacttttcgt tcgcgtttct ggccttttt
//
LOCUS       DAOSPX010000382         2872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_31488_length_2872_cov_3.643237, whole genome shotgun sequence.
ACCESSION   DAOSPX010000382 DAOSPX010000000
VERSION     DAOSPX010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31488_length_2872_cov_3.643237"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLZ75_13435"
     CDS             complement(<1..654)
                     /locus_tag="PLZ75_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73442.1"
                     /translation="MKRTIAVLPLLLLSVLLAAQAPFPTRNETAQFPVSTTCVVLEDD
                     AFSLFNSYIRDAVQEYWTLTPFEFIDQEEFGRRRNNPDYSFIMLTETTFDKDKSGSVF
                     SFINLVQGKNVADIGKMPEICAVPLAPAGDDGLDYGYKLGAILLFMQKHAALIVAEPS
                     KTGRKYLSFYNENIPRMSGKTILVRQEDLSPAISDPDDIRAIYPGKVEIVTEEEMVKA
                     "
     gene            complement(660..1424)
                     /locus_tag="PLZ75_13440"
     CDS             complement(660..1424)
                     /locus_tag="PLZ75_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73443.1"
                     /translation="MKSMFRKFGFAAFFLSVLPFTGSIAQEREGMTEYEPGFKFKDGI
                     YLNFEQVRNNDPIPKAKILTSADYNDKEFFKDLLASDKIYFYDNLGIRQEVDKGNIWG
                     YSRNGILYIQVQGNFNRITFVGSISHFVADIMTYDSRYYNPYGYGGYYDPYYYRYPYY
                     SPYSYYGNYYYPYGSYYSPYSRNLARSEIKQFIVDFDTGKVLDFDLENTQLLLMKDAQ
                     LYEEFVQLPRKKKKELMFVYIRKFNEKNPLYLPVNE"
     gene            1591..>2872
                     /locus_tag="PLZ75_13445"
     CDS             1591..>2872
                     /locus_tag="PLZ75_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS73444.1"
                     /translation="MPKEKGVVYDADPGQLPGEFYENHRRKLEKFFYVSISLLILAVI
                     FLMLNLALPAGILIILTIITALFLTRQVAGIFYSVRALLRDISSANESKDNVITDFSH
                     KIREPLNNLVFITDMLMGSELGQKEKDLLETFVASTKNMVTSVNELTMQSAQSISFES
                     RKHIKFNLLSTIQNTIELYDLKDRTNIRFTLNRKEHFDFDCSGDPIILKQILLDIFNN
                     IENQVQDQTVKVAIDIRKIRETGSEVFVGLRIRTDSRISLINEKELQHSLAARLITNR
                     RGLFSQELGQDHSVLNISLPFTIALSEHRLKVVTPETAESEKKTKERRELKDIDILLV
                     EDNLINQKITMLTLQPLVKNIDTASNGMEAIEKVSSGNYDIILMDIQMPVMSGLVAAE
                     RIRAMEAGTDSHIPIIAITANAMLGDKEKCISAGI"
BASE COUNT          851 a          640 c          619 g          762 t
ORIGIN      
        1 ggccttcacc atttcctctt ctgtcacaat ctcaaccttc cccggatata tggcccttat
       61 atcatcaggg tctgaaatcg cgggggaaag atcttcctgc ctgacaagaa ttgttttgcc
      121 ggacatccgg ggaatatttt cattatagaa actgaggtac ttccttccgg tttttgaagg
      181 ctctgcaacg ataagggccg catgtttctg cataaacagc agaatggccc cgagtttata
      241 tccgtaatcc agcccgtcgt ctccggcagg tgcaagtgga acagcacata tttcgggcat
      301 tttcccgata tccgccacat tcttgccctg aaccaggttg atgaaactaa ataccgaccc
      361 cgacttatcc ttgtcaaaag ttgtttcagt gagcattatg aacgaatagt ccggattatt
      421 ccttctcctg ccgaattctt cctggtcaat gaattcgaac ggagtcaggg tccagtattc
      481 ctgcacggca tccctgatat aggaattgaa aagggaaaag gcatcatctt ccagtacaac
      541 gcatgttgtt gaaaccggga attgggctgt ttcatttctt gtgggaaagg gagcctgagc
      601 ggccagcagt acggaaagca gcagcaaagg caggacggca atggttcttt tcatatttgt
      661 cattcattaa cagggaggta gagaggattc ttttcgttaa acttccggat atagacgaac
      721 atcagctctt ttttcttctt tctcggaagc tgaacgaatt cctcatacag ctgggcatcc
      781 ttcatcagaa gcagctgggt gttttccaga tcaaagtcaa gtaccttccc ggtatcaaaa
      841 tcgacgatga actgctttat ttcacttctt gccagattcc ttgaataggg ggagtaatat
      901 gatccgtatg gatagtaata gtttccgtag tagctgtagg gcgaataata aggatagcgg
      961 tagtagtacg ggtcataata acctccatag ccataagggt tgtaatacct tgagtcgtaa
     1021 gtcatgatat ccgccacgaa atggcttatg ctgcccacaa atgtgatcct gttgaaattg
     1081 ccctgtacct gaatgtataa gattccgttt cttgaatatc cccagatatt gcccttgtca
     1141 acctcctgcc taattccgag attgtcgtaa aaatagattt tatcggatgc cagaaggtcc
     1201 ttgaaaaatt ccttgtcgtt atagtcagct gatgtaagga tttttgcctt tggtatcggg
     1261 tcattgttcc ttacctgttc aaaattaaga taaataccgt ccttgaattt gaagccgggc
     1321 tcatactcgg tcatcccttc ccgttcctgc gcaattgatc ctgtaaaagg cagaacagaa
     1381 agaaaaaaag ctgcaaaacc aaatttcctg aacatactct tcatcacaat aacctttatt
     1441 ttatctgttc aaatatcata ccgcaattgc aaatttaacc atttacccgt aagcattgaa
     1501 atttcagcga gtgtatattt ctcatatatt ttattaaatt tgcattgatg tcgataaaag
     1561 gaatttgtgt ttagtcaata agctctcgcc atgccaaaag aaaaaggggt tgtttacgat
     1621 gctgatccgg gtcagctgcc cggggaattt tatgaaaatc acagaaggaa actggaaaaa
     1681 ttcttttatg tcagcatatc cctgctgata ctggcagtca tcttcctgat gctcaacctg
     1741 gcacttcctg caggcattct tataatactg accataataa cagcactgtt cctgacaaga
     1801 caggttgcgg gaatttttta ctccgtcagg gcccttctca gggatatttc atcagccaac
     1861 gaaagcaagg ataatgtaat caccgatttc agccataaga taagggaacc cctgaacaat
     1921 cttgttttca taactgacat gctgatggga tcggaactcg gccagaagga gaaggatctc
     1981 ctggagacat tcgtggcatc caccaaaaac atggttacct cggtcaatga actgaccatg
     2041 cagtctgccc agagcatcag ttttgaatcg aggaaacata ttaaattcaa cctgctgtca
     2101 acgatacaga acaccataga actgtatgac ctgaaggaca ggacaaatat caggttcacg
     2161 ctgaacagga aagaacattt tgattttgac tgttcaggcg atcccatcat tctcaaacag
     2221 attctgctgg atattttcaa caatattgaa aatcaggttc aggatcagac tgtaaaagtt
     2281 gccattgata tcaggaaaat cagggagacg ggaagtgagg tctttgtcgg actgaggata
     2341 agaacagaca gcaggatctc cctcatcaat gaaaaagaac tgcagcacag cctggctgcc
     2401 agactgataa caaaccggcg aggactgttc agccaggagc tcggacagga tcattcagtt
     2461 ctcaatataa gtctgccctt taccattgcc ttgtctgaac acagactgaa agtcgtgact
     2521 cccgaaacgg ccgaaagcga aaaaaaaaca aaggaacgcc gcgaactgaa agacatcgac
     2581 atactccttg tagaagacaa tctcatcaac cagaaaataa caatgcttac ccttcagccg
     2641 ctggtaaaaa acattgacac ggcgtcgaac ggaatggaag caatagaaaa ggtaagttcc
     2701 ggcaattatg atattatcct gatggatata cagatgcccg tgatgagcgg acttgtcgca
     2761 gcagaaagga tcagggccat ggaagccgga accgattcac atattcccat cattgcaata
     2821 acggcgaatg caatgctcgg cgacaaggaa aaatgcatct ccgcaggaat ag
//
LOCUS       DAOSPX010000383         2869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_31524_length_2869_cov_3.932836, whole genome shotgun sequence.
ACCESSION   DAOSPX010000383 DAOSPX010000000
VERSION     DAOSPX010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31524_length_2869_cov_3.932836"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2547
                     /gene="sprA"
                     /locus_tag="PLZ75_13450"
     CDS             <1..2547
                     /gene="sprA"
                     /locus_tag="PLZ75_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOS73445.1"
                     /translation="ALTEDFAQRKTIAVSNAGVARRGEKSHVWDLANLSVNYTYNEIY
                     RRNTKTEIDLEKNYRGGLNYNYEAQPANVAPFRNVKMLNSPVFRLIKDFNFYYMPRSI
                     AFRTDLNRYYNEVKSRNISNPYLRITPTFRKDFEWTRMFDFKYDISRQLKFDFTSNNI
                     ARIDEPAGGVDRRRYSTEYNLWRDSVMTNLKNFGRTTNYNHYLNLTYTIPVNKLPLLS
                     WVNANARYGSDYTWLAGPVYPDSMNINIGNSIKNHNDLSLTAMASLTTLYNKSKFLRQ
                     IESNTRPDAAQRMKREYRTVTFSRSNVNFRAGIPRGIIHNLRTGDVKVRVTNKDGTEV
                     EGKLEVVNETRITFTTAEKVDGAQVDIEGRVPVKRSPVIITGEYMLRALMGLRNVSLT
                     MTSSQGQFLPGYMPGTNYLGMSDHNDVLAPGWPFILGYSDRNFFDRAASNGWLSRDTL
                     LNTPASYNNRYDISARAQLEPFPGMRIDVNADRRFLEGVSAYYIADYNGNFPDSTRNR
                     IVTGNFSISIISWGSTFEKISKDNDYVSPAFEKFKENLVVISSRRAAERAGIDPNYDP
                     DYDPQTGGEIKGPYKSGYGKTSGEVMIPAFIAAYTKTDPEKVSLETFPSVMKMMPNWR
                     FTFDGLSRFPFIQKTFRSVNISHQYRSTYQIGNYTTNLSFITDASGAGSLRDLQNNFI
                     QQYEIGVVTINEQFSPLINVDMNWKNSLMTRFEWRKSRTVSLNLTSNQIADARINELI
                     VSAGYRFDDVQIVLRTGGGQKSLKSDLNLRFDLSIRDNKTLARKLVEDVNQPVVGQKV
                     FTAGLTADYVLSDRFNLQLFADHTMNNPFVANTFPTSNTNFGFSLKFTLV"
     gene            2663..>2869
                     /locus_tag="PLZ75_13455"
     CDS             2663..>2869
                     /locus_tag="PLZ75_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HOS73446.1"
                     /translation="MKIPEDLLFSEEHEWIRIEGDTGFIGITDFAQGELGDVVFVEIE
                     TRGETLSRGDVFGTIEAVKTVSDLF"
BASE COUNT          860 a          629 c          683 g          697 t
ORIGIN      
        1 gccctcaccg aggattttgc acaaagaaag acaattgcgg taagcaatgc gggagtggcc
       61 cggcgggggg aaaaatccca tgtctgggat ctggcaaatc tgagtgtgaa ttatacatat
      121 aatgagatct acagaagaaa tacaaagacg gaaatagatc ttgaaaagaa ttaccgggga
      181 ggactgaact ataattatga agcccagccc gccaacgtgg ctcccttcag aaatgtgaaa
      241 atgctgaatt cacccgtttt caggctgata aaggatttca atttctatta catgcccagg
      301 agcatagcat tcaggaccga tcttaaccgt tattacaatg aagtcaagtc aaggaatata
      361 agcaatccct atctcaggat cacccctaca ttcagaaagg atttcgaatg gaccaggatg
      421 tttgatttca aatacgatat cagccgtcag ctcaaatttg actttacttc caacaatatt
      481 gccaggatag atgagcctgc ggggggagtt gacaggagga gatacagcac tgaatacaac
      541 ctgtggcgtg attcggtaat gaccaatctc aaaaatttcg ggcggaccac gaattacaac
      601 cattatctga atcttaccta taccattccg gttaacaagc ttcccctgct gtcgtgggtc
      661 aacgcaaatg ccaggtatgg atccgactac acatggcttg caggtccggt atatcctgac
      721 agcatgaata taaacatcgg caattcaatc aagaatcaca acgacctttc cctgactgcg
      781 atggcttccc ttacgacact ttacaataaa tcgaagtttc tcaggcagat agaaagcaac
      841 acccggccgg atgcagcgca aaggatgaag cgtgaataca ggactgttac attttcaagg
      901 tcaaacgtca atttcagagc cggcattccg aggggaataa tacacaatct cagaaccggc
      961 gatgttaaag taagggtaac aaacaaggat ggtacagagg ttgaaggcaa actggaagtt
     1021 gttaatgaaa cacggataac attcaccact gcagaaaagg ttgacggggc ccaggttgat
     1081 attgagggca gggttcccgt aaagagaagc cctgttatca ttacggggga atatatgctc
     1141 agggctctca tgggcttaag gaatgtaagc cttacaatga cttcctccca gggacagttc
     1201 ctgcccggct acatgcctgg aacaaattat ctcggaatgt cggaccataa cgatgttctt
     1261 gcaccgggct ggccgttcat actgggctac agtgacagga actttttcga cagggcagca
     1321 tcaaacggat ggctgtcaag ggacaccctg ctcaacacac ccgccagtta taacaaccgc
     1381 tatgacatat ccgccagggc ccagcttgaa cccttccccg gaatgagaat agacgttaat
     1441 gccgacagga gatttcttga gggagtgagt gcctattata tagcggatta caacgggaat
     1501 ttccccgaca gtacccggaa caggatagtt acgggtaatt tttccatttc aataatatcc
     1561 tggggaagca cttttgaaaa gatcagcaag gataatgatt atgtatcgcc tgcctttgaa
     1621 aaattcaagg aaaacctggt tgtcatttcg tcccgcaggg cagcggagag agccggaatc
     1681 gatcctaact atgatccgga ttacgatcct caaacgggcg gggaaataaa aggtccttac
     1741 aagagcggtt atggcaaaac ttcgggtgag gtgatgatac cggcattcat tgcggcctat
     1801 acgaaaaccg atcctgaaaa ggtcagtctt gaaacctttc cttcagtaat gaaaatgatg
     1861 ccaaactgga gattcacctt tgacggcctg tcgagattcc cctttatcca gaaaacgttc
     1921 agatccgtca atatctcgca tcagtacagg agtacctacc agataggcaa ctatactaca
     1981 aacctcagtt ttatcacgga tgccagcggg gcaggcagcc tgagggacct gcagaacaat
     2041 ttcatacagc agtacgaaat tggtgttgta accattaatg aacagttcag tccgcttata
     2101 aatgtggaca tgaactggaa gaacagtcta atgacacgct ttgagtggcg gaaatcccgt
     2161 accgtatccc tgaatcttac cagcaaccag attgccgatg caaggataaa tgagctgatt
     2221 gtaagtgcag gctacaggtt cgacgatgtt cagattgttc tcagaacggg agggggtcag
     2281 aaatcgctca aaagtgatct taacctgcgc tttgacctgt cgataaggga taataagact
     2341 cttgcacgga aacttgttga ggatgtcaat cagcctgttg taggtcagaa agtattcact
     2401 gcgggcctta ctgccgacta tgtactgagc gacaggttca atcttcagct gtttgcggat
     2461 cataccatga ataatccatt tgtggccaat acctttccca cttccaacac caatttcgga
     2521 ttcagtctca agtttaccct ggtgtgacaa agaggagcag gacgataata attttgacag
     2581 ccggggatat ctgttaagaa aaatatacta tttttggttg attcagttat ccaacgttgt
     2641 aagactaaaa attatcatca agatgaaaat accagaggat ttattgtttt cagaagagca
     2701 tgaatggata cgcattgaag gcgataccgg attcattggc attaccgatt ttgcccaggg
     2761 cgaactgggt gatgttgttt ttgtcgagat tgaaacccgg ggggaaactc tttcccgcgg
     2821 agacgttttc ggcacgattg aagccgtgaa gacagtatca gacctgttc
//
LOCUS       DAOSPX010000384         2861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_31654_length_2861_cov_4.092302, whole genome shotgun sequence.
ACCESSION   DAOSPX010000384 DAOSPX010000000
VERSION     DAOSPX010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31654_length_2861_cov_4.092302"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLZ75_13460"
     CDS             complement(<1..247)
                     /locus_tag="PLZ75_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOS73447.1"
                     /translation="MITTTLSDFRKDIKKYLDNVTQNFETLIINRGKDSGVVIISIDE
                     YNSLAATQHELSSKANEKRLDSAIEKLKSKKAFQKELL"
     gene            complement(374..565)
                     /locus_tag="PLZ75_13465"
     CDS             complement(374..565)
                     /locus_tag="PLZ75_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73448.1"
                     /translation="MDQLFLLGANAAVAGTEAKTIEKEGAKYTGPGVTVDKNIITANA
                     PKASKLFGETICKILTDRK"
     gene            complement(869..1315)
                     /locus_tag="PLZ75_13470"
     CDS             complement(869..1315)
                     /locus_tag="PLZ75_13470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOS73449.1"
                     /translation="MFLVSEQCFSQQVVSSTGTSAEGANVRISWTVGEPMIGTFTGTS
                     VILTQGFHQSKLTITSLKEIDLPSFEVNVYPNPFFSQLQITVTKGDWNNLQYLLFSLD
                     GKLLQQKVALNQVETMNLESFAQGVYLLKVSGHHNQTIRTFLVIKN"
     gene            complement(1406..2734)
                     /locus_tag="PLZ75_13475"
     CDS             complement(1406..2734)
                     /locus_tag="PLZ75_13475"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOS73450.1"
                     /translation="MARDLSGNVLAGKSVRFRISILKGSTSPTAVYTEQHIKTTNSFG
                     LVELEIGKGSVPSGSFPGINWGNDDYFLKVEMDPNGGSAFQEMGTSQFLSVPYALYAR
                     ETGSSGLTLPYSGSVNTYDYGINQAFIVENTYSNGIGVWGRSGWSGVLGTSDTGIGVR
                     GQGPIALYGVSDVEKGEGLRVVCYDNSSQTIGVWATVASPIGYSGYFIGGRFFVSGNV
                     GIGDGAHSPDYRLDVRAAGTSSTGIAVFRNSSGDNKVLIRQNSNGCGAVAVYNTGGES
                     TIGLVGDGNSYINGGNLGIGTTSPNYKLDVRGTIGNNTTLYHSDRRWKTDFVPVRYGI
                     QDLMKLNSISYLWNVADYPDMGFDKNVQFGFIAQEFEQVIPELVTTDNEGYKSIDYVK
                     LTPLLVKAIQQQQEIIEGMKAENDRLKTENEQVISRIEKLEKLMGTSSMK"
BASE COUNT          786 a          642 c          522 g          911 t
ORIGIN      
        1 cgagcagttc tttttgaaat gcctttttac ttttcaattt ttctatagca gaatcaagtc
       61 gtttttcatt agcttttgat gaaagttcat gctgggtcgc ggccaaggaa ttatattcat
      121 caattgatat gatgacgact cccgaatcct tacctctgtt tattataaga gtttcaaagt
      181 tttgagtaac attgtcaaga tatttcttga tatcttttct gaagtctgat aatgttgttg
      241 ttatcatgga attcctattt atgtacatac tgaaatacaa aaatatgaac attcaataat
      301 atttccaact ctttcagcga atttttaact gtgtgttaac ggatggcgtt atgaaacgct
      361 gggtattgcg ggcttatttt ctgtctgtca atattttaca aatagtttct ccaaataatt
      421 ttgatgcttt tggtgcattg gctgttatta tatttttatc gacagtcaca cccggaccag
      481 tgtattttgc accttctttc tcaatagtct ttgcctcagt gccggcaaca gcagcattcg
      541 cccccaataa aaataactga tccataatct tccacctcta ttttatttag ttcaatttca
      601 gaagttgcaa atccgcctct gcttccataa cagcgaccca ctattgtgct tgctattatt
      661 gtactaaaac cggctttttc taaaacctct ttagtaataa acaattcttc gtctcgaaat
      721 ctttccggtg atataattag taaaactggg ttcatctgtt tcaggcaatt tgttagtaat
      781 cctgggttat aatgtggctt aagccggact cacgttaaaa ttagtgaatt tctgtccgtt
      841 ttttttagat cactaaatca gtgaggtgtc aattcttaat aaccagaaaa gtccggatcg
      901 tttgattatg gtgccctgat actttcaaca ggtatactcc ttgtgcgaaa gactcaagat
      961 tcatggtttc cacctggttc aatgcaactt tctgttgcag aagttttcca tcaagggaaa
     1021 aaagaagata ttgcaggttg ttccaatctc cttttgtgac agttatttgc aactgagaga
     1081 aaaaaggatt ggggtaaaca ttcacttcaa atgagggtaa atcaatttct ttcaatgaag
     1141 tgatggttaa cttgctttgg tgaaatccct gtgtcagaat aaccgatgtg ccggtgaaag
     1201 tcccaatcat cggttctccc acggtccatg aaatccggac attagcccct tctgcgcttg
     1261 ttcctgttga tgaaacaacc tgctgtgaaa aacattgttc tgaaacaaga aacaatatgg
     1321 ttaaaatgac ataataaatt aacttgttca tttctaaact ttttaaaata aaagttataa
     1381 aaaactatct ttcttttacc cgaacttatt tcattgaact tgttcccatc agcttttcaa
     1441 gtttctcaat gcggctgatg acctgttcat tttctgtttt caaacgatca ttttcagcct
     1501 tcattccttc aatgatttcc tgctgttgct gaattgcctt taccagcaaa ggggtgagct
     1561 ttacataatc gatcgactta taaccctcat tgtcggtagt gaccagctcg ggaattacct
     1621 gttcaaactc ctgtgcaatg aatccgaatt gaacattttt gtcaaatccc atgtcgggat
     1681 aatcggccac attccatagg tagctgatac tgttgagttt cattaaatcc tgaatgccgt
     1741 atctgacggg tacaaaatct gttttccagc gcctgtcgct atggtacagt gttgtgttgt
     1801 tcccaatggt tcctctgaca tccagcttat aattgggcga tgttgttcct attcccaggt
     1861 tcccgccatt gatatagctg ttaccatcac ctaccagtcc aatggttgat tcacctcctg
     1921 tgttatatac agctacagcg ccacaaccat tggagttttg acggattaac actttgttgt
     1981 cacctgatga attcctgaaa acagcaattc cggtgctgga ggttcccgcg gcacggacat
     2041 ccaggcgata atcaggagaa tgagccccat ctccaatacc tacattgccg ctgacaaaaa
     2101 acctgccacc gataaaataa ccggaataac ctataggtga agctactgta gcccatactc
     2161 ctattgtctg tgaggagtta tcgtaacaaa ctacacgcag accctcaccc ttctcaacat
     2221 cagaaactcc atataaagcg attgggcctt gacccctcac cccgatacct gtatcagagg
     2281 tccccaatac gccagaccaa ccactccttc cccagacacc aattccatta ctatatgtat
     2341 tttcaactat aaaagcttgg ttaataccat aatcataagt attcacacta ccactgtatg
     2401 gaagtgttaa cccgctgctg cccgtctcac gggcatacag ggcatagggt acgctcaaaa
     2461 actggctggt tcccatctcc tggaaggcag agcccccgtt gggatccatt tccactttca
     2521 gaaaataatc atcattgccc cagttgattc cggggaaaga tcctgaaggg acacttccct
     2581 tcccgatttc aagttccacc aatccaaagc tgttggtggt tttgatatgc tgttcggtat
     2641 ataccgccgt tggggaggta ctgcctttca ggatactgat cctgaatctt acacttttgc
     2701 cggccagcac attccccgaa aggtcacgag ccactgcctg gtacttgaaa gactgaggtg
     2761 cctgagcagc cacgttaagc caaatcatgc ataacatggt caagataaga aaggatttca
     2821 tgacattaag atttatatat aagaaagtta cgactatttc t
//
LOCUS       DAOSPX010000385         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_31719_length_2856_cov_4.446626, whole genome shotgun sequence.
ACCESSION   DAOSPX010000385 DAOSPX010000000
VERSION     DAOSPX010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31719_length_2856_cov_4.446626"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /gene="gldN"
                     /locus_tag="PLZ75_13480"
     CDS             complement(<1..620)
                     /gene="gldN"
                     /locus_tag="PLZ75_13480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOS73451.1"
                     /translation="MRRKISFGILILAFAASAAGQQSFGDIYKKSISEAKKIEYPHLR
                     EADAIWSKRYYRLIDLRERINQPLYYPTTPTLDGRMSFINVVLDEIKKGNITAYSAID
                     INVPTTYQEIETQMGAETRIQQIQIDAAGNTRDSTITEQAKPEEIKQLLLYEEWFFDK
                     KLGKLDVRIIAICPYYMGYDADLGRSLRKALFWVRFDEIRDILARK"
     gene            complement(635..2236)
                     /gene="gldM"
                     /locus_tag="PLZ75_13485"
     CDS             complement(635..2236)
                     /gene="gldM"
                     /locus_tag="PLZ75_13485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOS73452.1"
                     /translation="MSHEKLSPRQKMIGMMYLVLTAMLALNVSKEAVEAFKKVDNSLT
                     LTIANYAAKNDLIYKEFDRAAAENPAKAGNYRKTAYEVKERADELFNYMQGLKIEIIK
                     VAERDENTPAVRGNEVIINEVKKIDENNVPSQILIGAHENGKAFALKTMLVDFREFLI
                     ETLEGKNPSAEEALRKSLNTDDGRDPSGQPERWENLTFHNLPLVAVITVLSKMQVDIR
                     NAETEVLNSLYTQIDAASFKFNKLEAVVIPDANYVTLGGNYSAKVFISAIDTTQKPII
                     LVGDQEIPVDNSGKGIYTVRPTTTGAKPWGGVIKMKAPTGEEISYPFRSSYSVGVPNV
                     VVSPTAMNVMYMGIANPIDVSVPGVSPDNVKIKVVNGNFTTERVRRAGGEYFRGNWAV
                     RPAKAGQNVQVIVSATDASGKTTSYSPIEFRVKTIPKPEARFAGQNGGTISRNTAMAQ
                     QGVFALLPDFDFDLQYKITGFSMLYTDRMGDFEEPSSSSSLTPKQKELLGRLTRGKYL
                     TIKDIKAVGPDNRTVDLSPMLFKID"
     gene            complement(2274..>2856)
                     /gene="gldL"
                     /locus_tag="PLZ75_13490"
     CDS             complement(2274..>2856)
                     /gene="gldL"
                     /locus_tag="PLZ75_13490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOS73453.1"
                     /translation="GGGYGGGAGSAALAKFDEMLEKAEITPELFQKLGAGMQKLGDAT
                     SNISAMNDIAQASNKYMNTMNNANDALSRLAQSYQEATNTVNATGSSYNTIAETIAEM
                     QAGGKSYQQQLEVLNKNLAALNAVYELQKKGADDHIKESESLYQGLQSLMKNLTESSD
                     DTQRYRDQVARLNENLAALNNVYGNMLAAMNVK"
BASE COUNT          604 a          694 c          684 g          874 t
ORIGIN      
        1 tctttcctgg ccaggatatc cctgatctcg tcaaatctga cccagaacag ggccttacgc
       61 agcgatctgc cgagatcggc atcatagccc atataataag ggcagatggc gattattctg
      121 acatccagtt ttcccagttt cttgtcaaaa aaccattcct cgtacagcag gagctgcttg
      181 atctcctcag gcttcgcctg ttctgtaatg gtggagtcgc gcgtattccc cgcagcatca
      241 atctgtatct gctggatacg ggtctcggca cccatctggg tctcaatctc ctggtatgtt
      301 gtcggcacat ttatgtcgat cgcggaatag gctgttatgt ttcccttctt gatttcatca
      361 aggacgacat tgataaagct catccttccg tcaagagtcg gagttgtcgg gtaatacagc
      421 ggctgattga tcctttcccg caggtcgatc aggcggtaat acctttttga ccatatggca
      481 tccgcttccc tgaggtgagg atattcaatc tttttggctt cagaaattga tttcttgtat
      541 atatcgccga atgattgctg gcccgcggca ctggctgcaa aagccaggat taaaattcca
      601 aaagatattt tccttctcat aatatcggtc tttactagtc aattttaaac aacatgggtg
      661 acaggtcaac agtcctgtta tcaggcccca cggcttttat atccttaata gtcagatatt
      721 ttcctcttgt gagacggccc aggagctctt tctgcttggg tgtgagtgaa ctgcttgagc
      781 tgggttcctc gaaatccccc atcctgtcgg tatacaacat tgaaaatccg gtgattttgt
      841 attgaagatc gaagtcgaag tcgggcagca acgcaaatac accctgctgg gccatggcag
      901 tgttgcggga tattgttccg ccgttctgtc cggcaaagcg tgcttcaggt ttcggtattg
      961 tctttaccct gaattcaatg ggcgaatatg aagtggtttt gccactggca tcggtagcag
     1021 acacaattac ctggacattc tgtcctgcct tggcaggcct taccgcccag tttccgcgga
     1081 agtattcacc tccggccctt ctgacccttt cagtggtgaa attgccgttt accactttga
     1141 ttttcacatt gtcgggactg actcccggta ccgagacgtc aatgggattt gcgataccca
     1201 tgtacatgac gttcatggct gtgggtgaca ctaccacgtt cggcacgccg accgagtagg
     1261 acgacctgaa gggataactg atttcctcgc ctgtgggggc cttcatcttt ataacgccac
     1321 cccagggttt tgcaccggtt gtagtgggtc tgacggtata gatacccttg cccgaattgt
     1381 ccacgggtat ttcctggtcg cctaccagta tgataggctt ctgggtcgta tctatagcgg
     1441 aaataaagac ttttgcagag taattgccgc ccagggtaac gtagtttgca tcggggatga
     1501 caacagcctc gagcttgttg aatttgaatg aagcggcatc gatctgggta tacaggctgt
     1561 taagcacctc cgtctcggca ttcctgatgt caacctgcat cttcgagaga acggtgataa
     1621 ccgcaaccag ggggaggttg tggaatgtaa ggttctccca cctttcaggc tggccgctgg
     1681 gatccctgcc gtcgtctgta ttcaggctct tgcgcagggc ttcctcggca gaggggttct
     1741 ttccttccag tgtttcaatg aggaattcgc ggaaatcgac cagcatcgtt ttaagggcaa
     1801 aggcttttcc gttttcatga gcccctatca ggatctggga aggaacgtta ttttcgtcaa
     1861 tctttttgac ttcattgatt ataacttcat tgcctcttac ggcaggggtg ttttcatccc
     1921 tttcggctac cttgatgatc tcaatcttaa gcccctgcat ataattgaat aattcatccg
     1981 ccctttcctt tacctcatat gcggttttgc ggtagtttcc cgccttggcc ggattttcag
     2041 ctgcagccct gtcaaactcc ttgtaaatga gatcattctt tgcagcataa ttggcaatgg
     2101 taagagtcag actattatct acctttttaa aagcttccac ggcttccttt gatacgttaa
     2161 gtgcaagcat ggcggtgagc accaggtaca tcataccgat cattttctgc cgcggggata
     2221 acttttcgtg agacatgttg tgggagttta gttaactaaa gcagatgttt acgttattta
     2281 acattcattg ctgcaagcat atttccgtat acattgttga gagctgccag gttttcgttg
     2341 agtctggcaa cctggtcacg gtatctctgt gtgtcatccg aggattccgt aagatttttc
     2401 atcagagact gaaggccctg ataaagtgac tcagattctt ttatatgatc gtcggctccc
     2461 tttttctgaa gttcatatac ggcattcagt gcggccaggt tcttgttcag tacctcaagc
     2521 tgctgctggt aggattttcc gccggcctgc atttcggcaa tggtttcggc tattgtgttg
     2581 tatgatgatc cggtggcatt gactgtattg gttgcctcct ggtaggactg tgccagtctg
     2641 gaaagagcat catttgcatt gttcatggta ttcatgtact tgtttgatgc ttgtgctata
     2701 tcgttcatgg cacttatatt tgaagttgca tcgcccagct tctgcatccc ggcacccagt
     2761 ttctggaaaa gttccggtgt tatttcggct ttctcgagca tttcatcgaa ttttgccaga
     2821 gctgctgatc ctgcgccccc gccatatccg ccaccg
//
LOCUS       DAOSPX010000386         2817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_32325_length_2817_cov_3.757422, whole genome shotgun sequence.
ACCESSION   DAOSPX010000386 DAOSPX010000000
VERSION     DAOSPX010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32325_length_2817_cov_3.757422"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1114
                     /locus_tag="PLZ75_13495"
     CDS             239..1114
                     /locus_tag="PLZ75_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS73454.1"
                     /translation="MKRITRRQFIGKSAAGIGSAVLASHFSADLSAIAPPRPVRYPLG
                     FQVWTIREQVIRDFAGTMKMMAGIGYRSVEMCSPPGYELSGFGPLMKMSAREMKQIVN
                     DNGLLLESTHYTMDELRKHLDERIGFAAGSGQKQMILSSFGLPGNATMSDWMRAADEL
                     NRIGEKTKKAGIQTGFHNHHTEFGEIDNVLIYDALLNQFDPELVKMQFQVAVINIGYK
                     AADYFNKYPGRFISAHLADYSILDRKNVALGKGIVDWKEFFASAGKGGLKNIFVEMDQ
                     DTFSDSALFIRNLQY"
     gene            complement(1139..>2817)
                     /locus_tag="PLZ75_13500"
     CDS             complement(1139..>2817)
                     /locus_tag="PLZ75_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOS73455.1"
                     /translation="QKILGGLFLFSNGLAFAANSADFVYYRFVFKRATSDVFRTFEGE
                     PGVVKIFFKFVIDYWPVTFFCFFIWFLMAYLYGRIKAEKPQPSSRPAYVITNILFVPL
                     VAGLVVGGARGSLNESTRPISNSNAARYVKNPTNVAIVLNTPFSIFRTSGKKPLDRKI
                     FFDDEKLKELYNPHFIPEHKGDIRKENVVIIILESFGREYIGALNKDLDGGNYSGYTP
                     FLDSLISVSLTFDVSVANGKKSIDAMPSILASIPSLETPYTISHYSNNRIEGLAAHLA
                     RKGYYTTFFHGALSGSMGFDSFARISGFQDYVSYESYPYQGDTDGMWGIWDEPFFRFF
                     GERMKEFPRPFLASIFSLSSHHPFKVPEKYKGKFPRGPVPIAEVVGYTDFALRGFFKS
                     ISEEDFFQNTLFVITADHTNESIHREYQNSYGLYSVPVILYKPGSDLKGIRKRIAQQI
                     DIMPTVLNYLNYDEEFIAFGNDLLDDSVESFAFNTNGSIYHLYMRDHILIMLDYKPIG
                     LYNFKTDRFLEENLLEKETELLSVMDNKLKAIIQSYNGRLIDNEMTAAGP"
BASE COUNT          806 a          623 c          625 g          763 t
ORIGIN      
        1 cacaaaagcg ggtaagcggc ttccctgaaa ttttttaatt ttttacggaa gccccggcca
       61 tagttcacgc taaaggtatt atatttaaaa taaatttcaa ccggcacaat tcataaatat
      121 tgttaaaata ctgtatatct ttatcttagt ttttaaattg cttcattcag ccagcaggag
      181 gaaaggcaaa gaataaagtt acaaaatact ctaaataaga atcttaaaaa aaagcaatat
      241 gaagagaata acaaggcgtc agtttatagg taaaagtgcc gcggggatag ggtcagcagt
      301 gcttgcatca catttctctg cggacctcag tgctattgct cctcccaggc ctgtaagata
      361 tccgctgggg ttccaggtat ggacaatccg tgagcaagtc atcagggatt ttgccggtac
      421 catgaaaatg atggccggaa ttggttacag gagcgttgaa atgtgttctc ctcccggata
      481 tgaactatca ggcttcggcc ctcttatgaa aatgtcagcc agggaaatga agcagatagt
      541 aaatgataac ggcctgcttc tggaaagtac gcactacacc atggatgaac tccgtaagca
      601 tcttgatgag agaatcggct ttgcagccgg atcgggacag aaacaaatga ttctgtccag
      661 tttcgggctt cccggaaatg ctaccatgag tgactggatg agagcggccg atgaactgaa
      721 cagaatcggc gaaaagacta aaaaagccgg catccagacc gggtttcaca atcatcatac
      781 cgaattcggg gagattgaca atgtgcttat ctatgatgcc ctgctgaatc agttcgaccc
      841 tgaacttgta aagatgcagt tccaggttgc tgtaataaac atcggataca aggctgcaga
      901 ctatttcaac aagtatcccg gaaggtttat ttctgctcac cttgcagact attcgatact
      961 tgaccggaag aacgttgctc tcggcaaagg cattgttgac tggaaggagt ttttcgcttc
     1021 agccggcaag ggaggcctga aaaatatctt tgttgaaatg gaccaggaca cattcagtga
     1081 cagtgctctg tttataagga acctgcagta ttaatgccag cagtggccac tttgtctgct
     1141 aaggtcctgc cgctgtcatt tcattatcaa tgagcctgcc gttgtagctc tgtattattg
     1201 ctttcagttt gttatccata acagacagca gctctgtttc cttttcaagc aggttctctt
     1261 ccagaaacct gtcagtcttg aaattatata atcctattgg cttgtagtcc agcattatca
     1321 ggatatgatc cctcatgtag aggtgatata tgctcccatt tgtattgaag gcaaaagatt
     1381 caaccgaatc atcaaggaga tcattgccaa aggcaatgaa ttcttcatcg taattgagat
     1441 aattgaggac agtgggcatt atatctatct gctgggctat ccttttcctt atccccttca
     1501 gatcgcttcc cggcttgtac agtataaccg gaaccgaata taatccatag ctgttctgat
     1561 actccctgtg aatcgactca tttgtgtggt cggcagtaat gacaaaaagc gtattttgaa
     1621 aaaaatcctc ttccgaaatg cttttgaaaa atcctctcag cgcaaagtca gtgtatccca
     1681 ccacctccgc aatcggcacg ggtcccctgg ggaacttccc cttatatttc tcaggtacct
     1741 tgaacggatg gtgggatgaa agtgaaaaaa tcgaagccag gaacgggcgt ggaaattctt
     1801 tcatcctttc accgaaaaac ctgaagaatg gttcatccca tattccccac atgccatcgg
     1861 tatctccctg gtaaggatag ctttcatagc tcacataatc ctgaaaacct gaaatgcggg
     1921 caaaggaatc gaatcccatt gatccgctca gggcaccgtg gaagaatgtc gtgtaatagc
     1981 ctttcctggc gagatgggcg gccaggcctt caatacggtt gtttgaatag tgtgatatgg
     2041 tatagggggt ttccagtgaa ggtattgaag ccagaatgga aggcattgca tcaatggatt
     2101 ttttgccgtt tgcaacagat acatcaaaag tcaggctgac actgatcagg gaatccagaa
     2161 atggggtata accggaataa ttgcccccgt caagatcttt attcaatgca ccgatatatt
     2221 cacggccaaa gctctcaagt attatgatca ccacattttc tttcctgata tcccccttat
     2281 gttccgggat aaaatgggga ttgtacaact ctttaagctt ttcgtcatca aaaaatattt
     2341 tcctgtcaag cggttttttc cccgaagtcc tgaatatgct gaacggagta ttgagaacaa
     2401 tcgccacatt cgtgggattc ttcacatagc gtgctgcatt gctgtttgat atgggccttg
     2461 tcgattcatt cagactgcca cgcgctcccc ccaccacgag tccggcaacc aggggcacaa
     2521 aaagaatatt tgttattaca taggccggcc tgctggaagg ctgaggcttt tctgccttta
     2581 tccttccgta gaggtatgcc atcaggaacc atataaaaaa acagaaaaat gtgacaggcc
     2641 agtaatcaat cacaaatttg aaaaatatct tcaccacccc gggttctccc tcaaatgtcc
     2701 ggaatacatc agaggttgcc cgcttgaaga caaaacggta gtaaacaaag tctgcgctgt
     2761 ttgcggcaaa agccagtccg ttgctgaaaa gaaaaaggcc gccgagtatt ttctgat
//
LOCUS       DAOSPX010000387         2810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_32438_length_2810_cov_3.655535, whole genome shotgun sequence.
ACCESSION   DAOSPX010000387 DAOSPX010000000
VERSION     DAOSPX010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32438_length_2810_cov_3.655535"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..613
                     /locus_tag="PLZ75_13505"
     CDS             323..613
                     /locus_tag="PLZ75_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73456.1"
                     /translation="MARLDPSNNKIIEKRFVLLKGRKDFNSDCLKGNPIYDGNTLRAD
                     PVVNIIIKEPYGPKSIGNLDVWNFDAGKWDAQRIAMGRMIETKSQQYPSDLQ"
     gene            complement(636..1928)
                     /locus_tag="PLZ75_13510"
     CDS             complement(636..1928)
                     /locus_tag="PLZ75_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS73457.1"
                     /translation="MKRTNISRRKFVKGLAGSAAGIAVLRGNPGISAMSYSRIIGANE
                     RINIGVIGCGGMANSHMEALLNMKQSDNIDITAVCDIYTKRLEKAKELTKAQAFKNYQ
                     EILQKKDIDYVLIATPEHWHYQMTLDAIGAGKHIYVEKPMTHTIEQAKDICNKIASSK
                     LKMQVGVQGMSDDSYETAHKMIEEGALGKVVMAHIDYSRNYIGDFWAYEIDPDARPGI
                     NLDWEAWLGPAPKRPWDPRRYFQWRRYWDYSGGIATDLFIHRVTRIIKSVGLSFPDYV
                     VATGGTWNFTNSVAEIPDTFNMMLDYPEKTTVMVVSSLANDMPIRHVIRGHEATLEFT
                     REGFTITPQEQSELAIISGTGETVKGTNLFTYKKTGAEDITLHHRNLIAAIRKNEPLK
                     CDQDLGFYGVVACMMGVESFRNRKYLKWDQQNKVIIES"
     gene            complement(2149..2379)
                     /locus_tag="PLZ75_13515"
     CDS             complement(2149..2379)
                     /locus_tag="PLZ75_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS73458.1"
                     /translation="MSLIIRNGEISGFECSGGYVLNGEGSLIYPGNHTRRYTGQPDVN
                     GPVSSIRFELLREGIEDYEYIWMLKESALKSW"
     gene            complement(2443..>2810)
                     /locus_tag="PLZ75_13520"
     CDS             complement(2443..>2810)
                     /locus_tag="PLZ75_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73459.1"
                     /translation="DISGQWDVSIDFYSGKGEHKLYLGKQEGHEIKGTHKSTFSLLDI
                     AGRVEGRNVKLQSYYRENAQSIPYIFDGNVEGDTISGVLYLGEYRSANFIAKRAESGR
                     RPMMPITIPSHGGKKSDSW"
BASE COUNT          764 a          659 c          504 g          883 t
ORIGIN      
        1 agaaatgacg cgaagccctt tcttctgcgc gtatgagcct gttaatccgg caaaaaccac
       61 tgccagaaac aatgcaatca gccttaaact tttgaaatcc ataaaaatca tacagtttaa
      121 attaatccgt tgtggttatc cccgtgatga ccgttttaaa gataattatt attcatgttt
      181 cacgtgaggt cttcatattc atcgttcagt taagggataa actccaaaat tcctggatgt
      241 aaaaagcaca tatatatgca gcttttcatc gtccggaaat gattttggag aatcaggccg
      301 gagggcttca gtcataccct cattggcgag gctggatccg tcaaataata aaattatcga
      361 aaaacgcttt gttcttctga aagggagaaa ggatttcaat tcagattgcc tgaagggaaa
      421 tccgatctat gatggcaaca ccctaagggc tgaccctgtc gttaatataa tcattaagga
      481 accttatgga ccgaaatcca tagggaatct tgatgtctgg aactttgacg ccggaaagtg
      541 ggatgctcaa aggattgcga tgggcaggat gattgaaaca aaatcacagc aatacccatc
      601 tgatttacag taggttttcc tgattcatac gaaaattagc tttcgataat caccttattc
      661 tgctggtccc atttcagata ttttctgtta cgaaatgact caactcccat catacaggct
      721 acaacaccat aaaaaccaag gtcctgatcg cactttaacg gttcgttctt cctgatcgcg
      781 gcaatcaggt ttctatggtg taaagttata tcttcagcac ctgttttttt ataggtaaaa
      841 agatttgtcc cttttactgt ttctcccgta ccacttatta tggctagctc tgactgctcc
      901 tgaggagtta ttgtaaatcc ttccctggta aattccagtg ttgcctcgtg tcctcttatc
      961 acatgcctta tcggcatatc atttgccaat gaagaaacaa ccatgacagt cgttttttcc
     1021 ggatagtcca acatcatatt aaaggtatcg ggtatctcgg ccacactgtt tgtaaaattc
     1081 caggttccgc cggtggcaac aacataatcc ggaaagctca ggccaaccga tttaatgatc
     1141 cttgtcaccc ggtgaataaa caaatctgtt gcaattccac ctgagtaatc ccagtatctt
     1201 ctccactgaa aatatcttct cgggtcccag ggacgtttgg gtgcaggacc aagccaggct
     1261 tcccagtcga gatttattcc cggtctggca tcaggatcga tttcataggc ccaaaaatca
     1321 ccgatataat ttcttgaata atcaatatga gccatgacaa ccttcccaag agccccttcc
     1381 tcaatcattt tatgcgcggt ttcatacgaa tcatcagaca tgccctgtac cccgacctgc
     1441 attttgagtt ttgacgatgc aatcttatta catatatctt tagcctgttc aattgtatga
     1501 gtcattggtt tttcgacata aatatgcttt ccggcaccta tggcatcgag ggtcatctga
     1561 taatgccagt gttcaggtgt ggctataagt acataatcta tgtctttttt ctgcaggatt
     1621 tcctgataat ttttaaaagc ctgtgctttt gtcagctctt tagccttctc cagcctcttt
     1681 gtataaatat cacatacggc tgtaatatca atattgtctg attgcttcat atttaacaaa
     1741 gcttccatat gactgtttgc catgccaccg caaccgataa caccgatatt gatcctttca
     1801 ttcgctccga tgatacgtga ataactcata gctgagatcc ccggattgcc tctcagtact
     1861 gcaattcctg cagcacttcc tgccaggcct tttacaaatt ttcttcttga aatgttagtc
     1921 cttttcatgt ttcgattgtc ttatttggtt agtaaatctt gcataacatc tgttgtggat
     1981 caaaatccat tttaaaaaga tacagtcttt tcaatttatc ctgtcagaaa ttcagaaaat
     2041 aaagataagt caatccggaa ttactttttc atgacaaata gtattctttt caccggttct
     2101 ataaggaaaa tccgggaaag aagacagtat ttaactgccc tggctgtttt accagctctt
     2161 caatgcgctt tccttcagca tccagatgta ttcataatcc tcaatacctt ccctcagaag
     2221 ctcaaacctt attgacgaca cggggccgtt aacatccggc tggcctgtat acctcctggt
     2281 gtgattgccc ggatatatca gtgatccttc cccgttcagt acatatcccc cgctgcattc
     2341 aaaccctgaa atttccccat tccggattat cagggacaaa aactggtttt atatctgatt
     2401 tttcactatt gaacagtcag gtcatgataa aaaccatcat ttctaccatg aatcgctctt
     2461 ctttcccccg tgggaaggta ttgttatcgg catcatggga cgtcttccgg attctgctct
     2521 tttcgcaatg aaattggcag aacggtattc ccccagatag agcactcccg aaattgtatc
     2581 accttcaaca tttccgtcaa atatatacgg tattgactgg gcattttccc tgtaataact
     2641 ctggagctta acattcctgc cctcaactct tccggctata tcaagcaatg agaaagtgct
     2701 cttatgggtt ccctttattt catgtccttc ctgttttcca agatacagct tatgttctcc
     2761 cttgcctgaa taaaaatcaa tgcttacatc ccactggccg gaaatatcga
//
LOCUS       DAOSPX010000388         2790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_32755_length_2790_cov_3.982450, whole genome shotgun sequence.
ACCESSION   DAOSPX010000388 DAOSPX010000000
VERSION     DAOSPX010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32755_length_2790_cov_3.982450"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="PLZ75_13525"
     CDS             complement(<1..1408)
                     /locus_tag="PLZ75_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS73460.1"
                     /translation="MKKQYLWFVLLFSMVIASSCEKELEQIPLSTASKAAVFGSQHGL
                     EMYVNSFYTILPGRKTNLDAMSDYLAVRTVPIFIQEGAFAATLSSEWTWTDLRNLNYF
                     IVNCKDPAVDIDVRKNYLGIAKFFRAYFYFEKIKRFGDVPWVGKPLDVSDTDILYGGR
                     DSRTLVMDSVLADINYACENIRLTTDPTRSTITKYLAYALKSRICLFEGTFRKYHTEL
                     NLQSTAAAWLSAAETAAKKVMDEGGFSVYTAGGPSKAYRALFTSDDPVASEVMLSAIS
                     DISLKILNDANWYWTSGTYGDKASFIRTFINTYLNIDGTPFTNNPDWPEMLFRDEVKG
                     RDMRLQQTIRMGDYKRIVGGTQIPGPPVFSYTFTGYQPIKWALDDMYYDSERLNTNSI
                     SIFRFAEVLLNYAEAKAELGTLTDADWAATVGALRARAGITGGLDSKPTVADPYLTAN
                     YFPGISDPVILEIRRERGI"
     gene            complement(1428..>2790)
                     /locus_tag="PLZ75_13530"
     CDS             complement(1428..>2790)
                     /locus_tag="PLZ75_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HOS73461.1"
                     /translation="PSNQRFAFFPSISAGWRISKESFWNVSPKIISDLKIRASYGSLG
                     NGNVSSYIYQEQFSISQSANILAGQLPQITRNPAVLPDGITWETSTTTNFGLDFSMLS
                     DRLRFVGDTYIRNTTDMFTIGVTLPALYGATAPKGNYADLQTKGWEASLSWRDHFDVA
                     SKPLNYNVRFTMADNKSVIKKYNNPDKLLTDYYVGQVIGEIWGYETIGFFVDDEDVAN
                     HAKQSPQMRASPTGIWYAGDIKFRDLNNDGYINIGTNRVSDPGDRRIIGNKSPRYTYG
                     INLGADWNNFFLSTFFEGVGKQDWYPSTEAEYFWGVYNRPYNNIPLWHLGNMWTPDNP
                     NAYLPRIMSRAASNATTRELGVPQTKYLQNVAYIRLKNIQLGYDLPKNMISRFGINNL
                     RVYISGEDIWSWSPLYKIVGKGHLDVGNIGPRDQVIRPGAGADGFNYPIMKSFTFGLS
                     IGI"
BASE COUNT          729 a          677 c          606 g          778 t
ORIGIN      
        1 caattcctct ttcccttctg atttccagga taaccggatc acttataccg ggaaaataat
       61 ttgctgtcag atagggatcg gccacagtgg gctttgaatc aagtccgcca gttattcctg
      121 ctcttgccct gagagctccg acagttgcgg cccagtcggc atcagtaagg gtgccgagct
      181 cagcttttgc ctcagcataa ttcagcagta cctctgcaaa acggaaaatg gaaatggaat
      241 ttgtattaag cctttccgaa tcatagtaca tatcatccag ggcccattta atcggctggt
      301 aaccagtaaa tgtataggaa aataccggcg gcccgggtat ctgggtgcca cccacaatcc
      361 gtttataatc tcccatgcgg atggtttgct gtagtctcat atcccttcct ttcacctcat
      421 ccctgaacag catctcaggc cagtcgggat tattggtaaa gggagttccg tcaatattga
      481 ggtaagtgtt tatgaatgtc cggataaagc ttgccttgtc tccgtatgta ccgcttgtcc
      541 agtaccagtt tgcatcattg agtatcttga gcgatatatc cgatatggcc gaaagcatca
      601 cttcactggc aacaggatca tcactggtaa acagtgcacg gtatgctttt gaaggtcctc
      661 ctgccgtata aaccgaaaag ccgccttcat ccatcacctt ttttgcagca gtctcggcag
      721 cggacagcca ggcagcggcc gttgactgca gattgagttc cgtgtgatat tttctgaacg
      781 tgccttcaaa caggcatatt ctcgacttca gggcataggc aagatatttg gttatggtgc
      841 tgcgtgtagg atcagtagtc agcctgatat tctcacatgc atagttaata tcagccagaa
      901 cggaatccat cacaagcgtg cggctgtccc gccccccgta aagaatatcc gtgtcggata
      961 catccagggg tttgccgacc cagggaacat caccgaacct ttttattttc tcgaaataga
     1021 agtatgccct gaaaaatttc gcaatgccca gataattctt ccttacatca atatcaacag
     1081 ccggatcctt acagttgacg atgaaataat taagattcct caaatcggtc caggtccact
     1141 ctgaactgag tgtggccgcg aatgcacctt cctgaatgaa tatcggcact gttctcaccg
     1201 ccagataatc cgacattgca tcaagatttg ttttccttcc cggaaggata gtataaaatg
     1261 agttcacata catttccagt ccgtgctgac ttccgaaaac cgcagctttc gaagctgttg
     1321 acagtgggat ttgttcaagt tccttttcac aggaagaagc tattaccatg gaaaacagga
     1381 gaacaaacca taaatattgt tttttcataa tgatcacact ttaaaaatta aatacctatc
     1441 gataaaccaa acgtaaagct tttcattatc ggataattaa atccgtcagc tcctgctccc
     1501 ggcctgatca cctggtctct cggaccaatg ttcccgacat ccagatggcc ctttccgaca
     1561 atcttgtaga gtggtgacca cgaccatatg tcttcacccg agatataaac ccgcaggtta
     1621 tttataccaa acctggagat catattctta ggcagatcat atcccagctg tatattcttc
     1681 agccttatat aggcaacgtt ctgcagatat tttgtctgag gcacgccaag ttcccttgtc
     1741 gtggcatttg aggcagccct tgacattata cgcggcagat atgcgttagg attatcggga
     1801 gtccacatat tccccagatg ccagagcggt atgttattat agggcctgtt atacacaccc
     1861 cagaaatatt cggcttccgt acttggatac cagtcctgtt tccccactcc ctcaaagaag
     1921 gttgacagga agaaattgtt ccagtcggct cccagattga ttccataggt atatctcggg
     1981 gatttgttgc ctattatcct tctgtcaccc ggatcggaaa ccctgttggt accaatgtta
     2041 atgtatccgt cattgttaag atccctgaac ttgatatcac ccgcatacca gatacctgtc
     2101 ggggatgccc tcatctgggg gctctgcttt gcatgattgg caacatcttc atcatcaaca
     2161 aaaaagccga tggtttcata accccagatc tcaccaataa cctggccaac gtaataatcc
     2221 gtaagcagtt tgtcaggatt attgtatttt tttatcaccg acttgttatc ggccatggtg
     2281 aagcgcacat tataattcaa aggtttcgaa gcgacatcaa aatgatccct ccagctcagg
     2341 gatgcttccc atccctttgt ctgcaggtcg gcatagttgc cctttggtgc cgtggcacca
     2401 taaagtgcgg gaagagtaac accgatggtg aacatatccg ttgtatttct tatataggta
     2461 tcgccgacaa atctcagacg gtcggaaagc attgaaaaat caagaccgaa attggttgtc
     2521 gtggatgttt cccatgtaat tccgtcagga agaaccgcag ggtttcttgt tatctgggga
     2581 agctgtcctg ccaggatgtt ggcggactgg gaaatggaaa actgttcctg atagatatat
     2641 gaactcacgt tgccattccc gagtgatccg taagaggccc tgattttcag gtccgaaatg
     2701 attttcggtg atacattcca gaacgattcc ttggatatac gccatcctgc ggatattgaa
     2761 gggaagaatg caaatctctg atttgaggga
//
LOCUS       DAOSPX010000389         2782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_32880_length_2782_cov_4.355336, whole genome shotgun sequence.
ACCESSION   DAOSPX010000389 DAOSPX010000000
VERSION     DAOSPX010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32880_length_2782_cov_4.355336"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLZ75_13535"
     CDS             <1..638
                     /locus_tag="PLZ75_13535"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOS73462.1"
                     /translation="LDEIFLVKYPKKYLRDSVESKLFGIGSEAMVVGSHDFYMGYALK
                     NDLMITLDNGHFHPTESVGDKISSLLLFYDELLLHMTRGVRWDSDHVTTLNEEIVLIA
                     QEIVRAKALGRVNIGLDYFDASLNRVGAYVLGTRAVQQAFLFALLEPHAALVKMEEQG
                     KTFQRLAMLELMKSKPFGAIWDYHCLKNNVPAGGDYIAEIEKYEKDVLLKR"
     gene            819..2180
                     /locus_tag="PLZ75_13540"
     CDS             819..2180
                     /locus_tag="PLZ75_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOS73463.1"
                     /translation="MKEKIIAVFDVGKTNKKVILFNYKLKVISELEEKFAETVDDDGF
                     PCDDIGHIEQWIRESVLRLVRSDKYDLTAVNFATYGATIVYLDAEGNRLTPVYNYLKP
                     MDERLQERIYRKYGGQDEFCRRTASPALGMLNSGMQIVWLKHEKPEQYSRVRHILHFP
                     QYLSFLLTGKIYTEHTSIGCHTALWDFDNMAYHPWVGNEGIVLPEPADAGTIDEIEIE
                     GKRIKVGIGIHDSSSSLVPYFSSSKGKFLLISTGTWCINMNPFNTEILTAAQLDHDCL
                     CYMSIAKQPVKSSRLFLGNFHENGVRKLSEYFKTRPDHYKSVKPSKKLIEICRARSAE
                     NRCFFDMLPYSRELREKPDLFIFNSFEEGYHQLMVELTELTAEAIDLVIPANDDIENI
                     YITGGFSANSLFRKLICASYPGKNVYTSEILNATALGAALVILRTMNPEASPVLNLGL
                     RRC"
     gene            complement(2200..>2782)
                     /locus_tag="PLZ75_13545"
     CDS             complement(2200..>2782)
                     /locus_tag="PLZ75_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS73464.1"
                     /translation="VFQGYLLISKENLSHFYPSVEGSSVFLVDGLPELSEFYGKILNE
                     RLSGYGFSAISAGEKLASFFRVTNTYLNVFAVFGTFGMILGITGLGFVLLRNYNIRKH
                     EFAFLSASGYTVERIRKLILRDQLIILAWGVVCGTVSGITATIPSLRSGSEMPLGLLA
                     AMILLVIITGTAVLYFSAGGIKSSELISLLRKE"
BASE COUNT          791 a          617 c          667 g          707 t
ORIGIN      
        1 cgcttgacga aatattttta gtgaaatatc cgaaaaaata tctcagggac tccgttgaaa
       61 gcaagctttt cggcatagga tccgaagcca tggtggtggg ttcacacgat ttctacatgg
      121 gatatgccct gaaaaatgat ctgatgatca ctctcgacaa cgggcatttc catccgacgg
      181 aatcggtcgg ggacaaaata tcctcgcttc ttttgtttta tgatgagctt cttctgcata
      241 tgacacgggg tgtcagatgg gacagtgatc atgtcacaac cctgaatgag gagattgtac
      301 tgatcgccca ggagattgtc cgggccaaag ccctggggcg cgtgaatatc ggactcgatt
      361 attttgatgc cagccttaac agggtaggtg cctatgttct cggaacaagg gctgttcagc
      421 aggcatttct ctttgcattg ctcgaacccc atgcggcact cgtaaaaatg gaagagcagg
      481 gaaaaacctt tcagcggctt gccatgctgg agcttatgaa atccaaacca tttggtgcaa
      541 tatgggacta tcactgcctg aaaaacaatg tgccggcagg aggggattat attgctgaaa
      601 tagaaaaata cgaaaaggac gttcttctta aaagataatt tgtatattga tgttttccgg
      661 ataatccgca gggctgttca tacaccaggc agccatgcgg agatccatcc cgctttgcca
      721 ccggcagaat aaacaggacg ggagacagtc gtgctccccg tattgctgtc ctgtacagac
      781 cagtcaaagg gtacttaatt aaacatcagg aaccgtgtat gaaagaaaag attattgctg
      841 tatttgatgt aggcaagacc aataaaaagg ttatcctctt caattataaa ctgaaagtga
      901 tttcagagct ggaggaaaaa tttgctgaaa cagttgatga tgacggattt ccatgtgacg
      961 atatcggaca tattgaacaa tggatcaggg aatctgttct caggctggtc aggtcggata
     1021 agtacgatct tactgcagtt aactttgcca catacggagc caccattgtg tatcttgatg
     1081 ccgaaggaaa ccggctgacg cctgtttata attacctcaa gcccatggac gaaaggctgc
     1141 aggaaaggat atacaggaaa tacggcgggc aggatgaatt ctgcaggaga accgcgtctc
     1201 ccgcactggg aatgctgaac tcaggaatgc agatagtatg gcttaaacat gaaaaaccgg
     1261 aacagtattc aagagtcagg catattctcc attttccgca gtacctgtct tttcttctta
     1321 caggaaagat ttatacggag cacacttcca taggctgcca cactgccctg tgggattttg
     1381 acaatatggc ctatcacccc tgggtgggga atgagggaat tgttcttccg gaaccggctg
     1441 atgcaggtac aattgatgag attgaaattg aaggtaaaag gataaaggtc ggaataggca
     1501 ttcacgacag ttcctcatcg ctggtaccat atttttcgag cagcaaggga aaattcctgc
     1561 tgatttcgac aggcacatgg tgtataaaca tgaatccctt caatacggaa atactgacag
     1621 ccgcccagct tgaccatgat tgtctgtgtt acatgagtat tgcaaaacag cctgtcaagt
     1681 cttcccggct cttccttggc aactttcatg aaaatggggt aaggaagctg tcagaatatt
     1741 tcaagacacg gcctgatcat tataagagtg taaagcccag caaaaagctg atcgaaatct
     1801 gcagggccag atcggcagaa aacagatgtt ttttcgacat gctgccatat tccagggagc
     1861 tgagggaaaa accggatctg tttattttta attcctttga agaaggttat caccagctta
     1921 tggtggaact caccgaactt accgcagaag cgattgatct tgtgatccct gcgaatgatg
     1981 acattgaaaa tatctatatc accggtggtt tctccgcgaa cagcctgttc cgcaagttga
     2041 tctgtgcctc atatcccggc aagaatgttt atacttcgga aatcctgaat gccacagcgc
     2101 tgggtgctgc acttgtaata ctccggacaa tgaatccgga ggcaagcccg gttctgaacc
     2161 tggggctcag gcgctgctga atgcaggtac aggtagaagc tattcctttc gcagaaggga
     2221 gatcagttca ctgcttttta ttcctccggc cgaaaaataa agcacagccg ttccggtgat
     2281 aattaccagg agtatcattg ctgccagcag tccaagcggc atttcgctcc cgctgcgtaa
     2341 tgaaggtatt gttgcagtga tgcctgatac ggtaccgcat accacccccc aggcaagaat
     2401 gatcagctga tccctgagaa taagcttcct tattcgttcc acagtgtagc cgcttgccga
     2461 aagaaaggca aattcatgct tcctgatatt atagttccgc agaagaacaa atcccagtcc
     2521 cgttattccc agtatcattc cgaaggttcc gaatactgcg aacacattca gataggtatt
     2581 ggttacccgg aaaaaggatg caagcttttc accggcactt attgcagaga agccgtaacc
     2641 ggacaaacgc tcattgagta tcttcccgta aaattccgat aattctggca gtccgtcaac
     2701 aagaaagact gaactgcctt ccacagacgg gtaaaaatgt gagaggtttt ctttgctgat
     2761 cagcagatat ccctggaaaa ca
//
LOCUS       DAOSPX010000390         2763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_33163_length_2763_cov_3.709010, whole genome shotgun sequence.
ACCESSION   DAOSPX010000390 DAOSPX010000000
VERSION     DAOSPX010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33163_length_2763_cov_3.709010"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2171
                     /locus_tag="PLZ75_13550"
     CDS             <1..2171
                     /locus_tag="PLZ75_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73465.1"
                     /translation="DPFNFRMVIMDLEKFRILHSIPVGRFPLGIDLSPDEKYAYVANT
                     GIFDYPLAPGLDKDNIEEAGLDFPPYGFPSEESEKGTEIGGRFIPGLGSPHVPEAVSV
                     WTISLDSNKVISREKTGYRMGAMIEGLEVEGGSSPNSIAAGERVFVSNATNDNISVLD
                     PQSGRVTDTIRLTIDPRIDRYRGMIPFGIELSNDGSRLYIALSGMNAVAVIDTRTLNV
                     LGYIPTGWFPTKLKVSPDDRVLYVVTARGLGSGPNGGKDFIPTKNGTYVGDIMLGTFE
                     KINLENIDYKATTQEVINNTFREIIVRDDKKNPLPPASGIRKSPIRHIVYITKENRTY
                     DEIYGELEGASGDPSLARYGKNAAVISRDGKRQVMNVNVMPNHQKIAGEFAVSDNFYC
                     DSDASVHGHRWMVGTYPNEWVEINSSMKMTRNIFSSAPGRKYVAGSSGAVYPEDYNEA
                     GGMWEHLSRNGISFFNFGLGFEFSAAIEEQWHKYSGVKMGVMFPMPKPLFDRTSRKYA
                     TYNTSIPDQFRIDMFEEELREKWLSGKEEFPSVITMMIPNDHGSSERPGDGYPFRESY
                     MADNDLAVGRVVETLSHTKWWPEMLIIITEDDAQDGRDHVDAHRSLLIMISPWIKRGY
                     VSHKHANFGAILKTIYTILDIDPLNQFDATATLLQDFFSLNPDFTPYKAVEVDRRIFD
                     PQKALDPYDKEFRWESLAESPEIDNRDDFRSSHREQADNN"
     gene            2330..>2763
                     /locus_tag="PLZ75_13555"
     CDS             2330..>2763
                     /locus_tag="PLZ75_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS73466.1"
                     /translation="MTEGDTEKVKCLIIGSGPAGYTAAIYAARANLSPVLYTGLQMGG
                     QLTTTTEVENYPGYPDGITGPEMMEHFKKQAERFGTDVRIGMATAADFSAGNLIVTID
                     GNRKINADTVIIATGATARYLGIEAETKYAGMGVSACATCDG"
BASE COUNT          814 a          571 c          705 g          673 t
ORIGIN      
        1 tcgatccgtt taattttcgt atggttataa tggatctgga aaaattccgc atactgcatt
       61 ccattcctgt cggaagattc cctctcggaa tcgacctgtc gcccgatgag aaatatgcct
      121 atgttgcaaa tacgggaatc tttgactatc ctctggcacc gggacttgat aaagataata
      181 tagaagaggc cggacttgac tttcctccct acggctttcc ttcggaggaa tcagaaaagg
      241 gaacggagat cggcggaaga tttattccgg gactgggcag tccacacgtt cctgaagcag
      301 tatccgtctg gaccatcagt ctcgacagca acaaggtgat atcgagggaa aaaacaggct
      361 atcgcatggg ggcaatgatt gagggtctgg aggttgaagg cggatcaagc cccaattcca
      421 ttgctgcagg tgaaagagta tttgtgagca atgccaccaa cgataatatt tccgttctcg
      481 atccgcaaag cggcagggtg acagatacaa tacgactgac aattgatccg cggatagatc
      541 gctacagggg gatgataccc ttcggaatcg aactgagcaa tgacggatca aggttgtaca
      601 ttgcactgag cggtatgaat gccgtggccg ttatagacac ccgcacactg aatgtactgg
      661 gatatattcc gacaggctgg tttcccacaa agctaaaggt ttccccggat gacagggtgc
      721 tgtatgtggt gacggccagg ggactcggtt ccggccccaa tggtggaaag gactttattc
      781 ccacaaaaaa cggtacctat gtaggtgata taatgctcgg gacctttgaa aaaatcaatc
      841 ttgaaaatat tgattacaag gcaactacgc aggaggttat aaacaatacc ttcagagaga
      901 taattgtgag ggatgataaa aagaatcccc ttcccccggc ttccggaata agaaaaagtc
      961 ctatccggca tattgtatac attaccaagg agaaccggac ctatgatgag atatacggtg
     1021 aactggaggg cgcctccgga gatccgtcac ttgcgcgata tggtaagaac gccgccgtaa
     1081 taagcaggga cgggaaaagg caggtaatga atgtaaatgt gatgccaaat catcagaaga
     1141 ttgccgggga atttgctgtc agtgacaatt tctactgtga ttccgatgct tccgtacacg
     1201 ggcatagatg gatggtcggt acttatccga acgagtgggt tgagattaac agttccatga
     1261 aaatgacccg gaatattttc agttcggcac ccgggcgcaa atatgttgcg ggttcctcgg
     1321 gagcggtata tcctgaagac tataatgaag caggtggcat gtgggaacac ctgtcaagaa
     1381 acggaataag cttctttaac ttcggcctgg gatttgaatt ctccgctgct attgaagaac
     1441 aatggcataa atattcaggg gttaagatgg gggttatgtt ccctatgccg aaaccattgt
     1501 ttgacaggac ttcacgaaaa tatgcaacat acaataccag tattcccgac cagttcagga
     1561 ttgacatgtt tgaagaggaa ctccgggaga aatggctgtc gggcaaggaa gaattcccgt
     1621 cagtcattac catgatgata cctaacgatc acggctcttc cgagagaccc ggcgatggct
     1681 atcctttcag ggagtcctat atggctgata atgatcttgc tgtcgggagg gttgttgaaa
     1741 cactgagtca tacgaaatgg tggccagaaa tgctgataat tattaccgag gatgacgccc
     1801 aggacggaag ggatcacgtg gatgcccacc gttcattgct gataatgata agcccctgga
     1861 tcaaacgagg gtatgtgtcc cataagcatg caaatttcgg ggcgatactc aaaaccatat
     1921 ataccatcct tgatattgat cctcttaacc agtttgatgc aacagcaacg ctgctgcagg
     1981 atttcttcag cctcaatcct gattttactc cctacaaagc ggtggaagtt gacagaagga
     2041 tatttgatcc gcagaaagca cttgatccct atgacaagga attcagatgg gaatccctgg
     2101 ctgagtcacc tgaaattgac aacagggatg atttcaggtc aagtcacagg gaacaggcag
     2161 acaataattg aatttataaa tacttttttt agttttgcgc gaaaatagaa aattcaggct
     2221 aaggtttcag aaatccgcaa tgcgggataa ttatatatta ttcagggaat aaaacagttt
     2281 taaaatggaa ttatttaaac caatggatct tacgggtgag agtgttaata tgactgaagg
     2341 tgacaccgaa aaggtgaaat gtctgataat aggatcgggg ccggccggct atactgccgc
     2401 aatttatgca gcaagagcca atctcagtcc tgttctttat accggactgc agatgggcgg
     2461 acaactcacc acaactactg aagttgaaaa ttatccggga tatcctgacg gcataacggg
     2521 acccgaaatg atggaacatt ttaagaaaca ggctgaaaga ttcggcaccg atgtccgtat
     2581 aggaatggct acagcagctg atttttcagc cgggaatctg attgtaacca ttgacggaaa
     2641 caggaaaata aatgcagata cggttattat agctaccgga gcgactgcca gatacctggg
     2701 aattgaagcc gaaacgaaat atgccggaat gggtgtgtcg gcctgtgcta catgcgacgg
     2761 ttt
//
LOCUS       DAOSPX010000391         2747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_33410_length_2747_cov_3.781947, whole genome shotgun sequence.
ACCESSION   DAOSPX010000391 DAOSPX010000000
VERSION     DAOSPX010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33410_length_2747_cov_3.781947"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLZ75_13560"
     CDS             <1..316
                     /locus_tag="PLZ75_13560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS73467.1"
                     /translation="DPLRGLSEMHRVLRREGAVMVLEFSMPRGGLFRRLYRFYFLRIL
                     PLIGKLFSGNDYAYTYLPGSVLEFPVNEHFIDLMEKAGFRSVKQKKLSGGVASIYTGL
                     KI"
     gene            379..1116
                     /locus_tag="PLZ75_13565"
     CDS             379..1116
                     /locus_tag="PLZ75_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOS73468.1"
                     /translation="MKKGSRHNILILLFLFVCITAPAQKQKPKNESWYDEKLLHFGFS
                     IGFNSMDFRITPSQDYLRKDSLYPEVSRLNPGINIQIVTDLRTGRYWDLRFLPGVSFG
                     QRSVRYYKVPGEKDGDPELFNVRQRLESSYLEFPLLLKYKGERLNNARPYVIGGLNFR
                     YDLAARKEFDDEKPVYIRLKRPDLYYETGAGLDFYLRYFKLSVELKMSNGLSDIIARE
                     GEARHPEFRNAIEKMRSQIWILAFHFE"
     gene            complement(1197..1967)
                     /locus_tag="PLZ75_13570"
     CDS             complement(1197..1967)
                     /locus_tag="PLZ75_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOS73469.1"
                     /translation="MLSKKKISFIVSLQKKKAREEHRLFTIEGDKLVREFLSAGQKVK
                     ILVAKPEFLNSLLPSHRQEIEEIIPCSYEELKRISSLKTPHNALAVVEMPEMKADSED
                     TAKGLAVALDFIQDPGNLGTIIRAAAWFGIRTIYCSENCVDVFNPKVIQASMGAILHV
                     KVIYTDLHVLFRNALRDKIKIYGATLDGSSVYSLEPENRGIIFFGNESRGISPDLATY
                     ITDRITIPSMDGSAAGIDSLNVGMAASVILSEFTRRSL"
     gene            2130..>2747
                     /locus_tag="PLZ75_13575"
     CDS             2130..>2747
                     /locus_tag="PLZ75_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73470.1"
                     /translation="MLLVFALFLLFSACHPTRYVPQDASLLDKNHVSVNREGINRGTL
                     EPFIKQKPNKRIFGMRFHLGLYNLSNVKKEGWPHSWLRKIGEEPVIYDPYLTGKSVEQ
                     IRSYVASKGYFDARVTDTVRTEKQKSEVFYNVALKSPYRIRSLHYEFADSLIGSWFYF
                     DSLNCLIVRGKRYDVDVLQAEQQRFEKFIKDRGFYGFSREHIYFRV"
BASE COUNT          725 a          584 c          650 g          788 t
ORIGIN      
        1 cgatccgctg aggggccttt cggaaatgca cagggtgctc agaagggaag gggcggtgat
       61 ggtactggaa ttttccatgc cccggggggg gctgttcaga cgtttgtacc gcttctattt
      121 cctcagaatt cttcctctta ttggaaaact gttttccggg aatgattacg cctacaccta
      181 tcttccggga tctgtcctgg aatttcctgt caatgaacat ttcattgacc ttatggaaaa
      241 agccggattc agatccgtga aacagaaaaa actcagcgga ggggtggcaa gcatttatac
      301 cggactgaaa atttaggtac aatatttcct gcctcttgca gtttattatt tgagaatttc
      361 agtaataatc aatccggttt gaaaaaaggg agccgacata atattcttat cctgcttttc
      421 ctgtttgtct gcataactgc accggcacag aagcagaagc ctaaaaatga atcatggtat
      481 gatgaaaaac tgcttcattt cggattcagt ataggtttca acagtatgga tttcagaatc
      541 accccgtcgc aggattatct gaggaaggat tcgctttatc ctgaagtcag ccgccttaat
      601 cctggtataa acatacagat cgttactgat ctcagaacgg gaaggtactg ggatttaaga
      661 tttcttccgg gtgtttcatt cggacaaaga agtgtaaggt attataaagt tcccggagaa
      721 aaggatggtg atcccgagct tttcaatgtc aggcagaggc tcgagtcatc ctatctcgaa
      781 tttccgcttc tcctgaaata caagggggaa aggctgaaca atgcgagacc ctatgttatc
      841 ggaggcttga atttccgcta tgaccttgcc gccaggaagg agttcgatga tgaaaaacct
      901 gtgtatatca ggctgaaaag gcctgatctc tattatgaaa caggagcggg tcttgatttc
      961 tacctgcgtt atttcaagct ttctgtagaa cttaaaatgt caaacggcct ttcggacata
     1021 attgccaggg agggagaagc ccggcatccg gaattcagga atgcgataga gaaaatgaga
     1081 tcgcagatct ggatactggc atttcatttc gaatagtcgt acagcagagt ttatttgcct
     1141 gtatccggtt gccgggtagc ttttacaggc tttggtttcc tggtaacttg ttctgattac
     1201 aggctccttc ttgtgaattc cgacagaatg acagatgcag ccatcccgac gttcagggaa
     1261 tctattcccg cagcggatcc atccatggaa gggatcgtta tcctatcggt aatatatgtt
     1321 gccaggtcag gagaaatgcc tcttgattcg tttccgaaga agatgatgcc cctgttttca
     1381 ggttcgaggg aatatactga gctgccgtca agcgtggcac cgtaaatttt tattttgtcc
     1441 ctgagggcat tccggaaaag aacatgcaga tcagtataga tgaccttcac gtggagaatc
     1501 gctcccatgc ttgcctgtat cactttggga ttgaaaacgt ccacgcagtt ttccgagcag
     1561 tatatggttc tgatgccgaa ccaggcagct gcccgtatta tggtaccgag gtttcccgga
     1621 tcctgaatga agtccagcgc cacagccagg ccttttgccg tgtcttcaga atctgctttc
     1681 atttcaggca tttccacaac cgcaagtgca ttgtgcggcg ttttcaggga agagattctt
     1741 ttaagctctt cgtaactgca gggaatgatt tcttcaattt cctgcctgtg actggggagg
     1801 agcgaattca gaaactcagg cttggccacc aggattttta ccttctgacc tgcagaaaga
     1861 aattccctta caagcttgtc gccctctatt gtgaaaagcc tgtgctcctc cctggccttt
     1921 tttttctgaa gggatacaat aaaactgatc ttttttttgc ttaacacctg aggattgctt
     1981 tttatgtgat aatattcagc aagttctccc gcttacattt taagcggagc gattccgtaa
     2041 atattattat tgatatattt gttcacttat gcatgataca aaatttgaaa aaaaatatat
     2101 caaataaaaa tacatgcccg ccgggatcaa tgctgctggt tttcgcattg ttcctgcttt
     2161 tttcagcctg ccatccaacg cgatatgttc cgcaggatgc gagcctcctc gataaaaacc
     2221 atgtttcggt aaacagggaa ggcattaaca ggggcactct cgaaccattc atcaagcaga
     2281 aaccgaacaa gaggattttc ggtatgcgct tccacctcgg gctctacaat ctttccaatg
     2341 tgaaaaagga ggggtggcct cattcctggc tcaggaagat aggagaggaa ccggtgattt
     2401 atgatcccta tctgaccggc aaatccgttg aacagatacg cagctatgtg gcatcgaagg
     2461 gttattttga tgccagggtg actgacactg tcaggaccga aaagcaaaaa tcggaagtgt
     2521 tctacaatgt tgctcttaaa tcaccctaca ggatacgcag tctgcactat gaatttgctg
     2581 attccctcat aggaagctgg ttttattttg actcactgaa ctgtttgata gtcaggggta
     2641 aacgctatga tgttgatgtc ctgcaggcag aacagcagag atttgaaaag tttataaagg
     2701 acaggggatt ttatggattc tcgagggagc atatttattt cagggtt
//
LOCUS       DAOSPX010000392         2735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_33620_length_2735_cov_5.125746, whole genome shotgun sequence.
ACCESSION   DAOSPX010000392 DAOSPX010000000
VERSION     DAOSPX010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33620_length_2735_cov_5.125746"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            559..1515
                     /locus_tag="PLZ75_13580"
     CDS             559..1515
                     /locus_tag="PLZ75_13580"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF000824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOS73471.1"
                     /translation="MPNPYLHRKVVVVGAGNVGATYSYALAQSGLADEIVLIDNNKDL
                     VKGQVLDLIHGQPFFPTVNIREGSRNDYDDAAVIVVTAGSAQKPGETRLDLLRRNAEI
                     VGSIAEDISARNKNGVMLIVSNPVDVMTWVAIKRSGWESGRVIGSGTVLDSARFRHLL
                     SKHCDVNVHNVHAYILGEHGDNQFAAWSMTHIGGINIDQYCHICRKCDNWMAEREKIE
                     QQVRDSAYHIISYKGSTHFAVGLAMVRITGTILRGENRVMTVSTILGGEFGLNDVCLS
                     VPSMVSDKGVSRIIESPLANDEAISLAGSAAILKEAIKSINM"
     gene            1638..>2735
                     /locus_tag="PLZ75_13585"
     CDS             1638..>2735
                     /locus_tag="PLZ75_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOS73472.1"
                     /translation="MKQPQLKIEKTGNITIFGRELIDENSVRQIKNCISSENDIAVLN
                     ADAHYGYGHPIGGAVAYRDKISLSGVGFDIACGNYAVRTDIPVKSVNIAGVMDEIVRR
                     ISFGIGRPNNEPVDHPVLEKINRAEFVPQRKLSRLAREQLGTVGSGNHFVDLFEDDDG
                     FLWTGVHFGSRGFGHRTTMGFIAMSRGYSFDEKVREGSMDSPPILFGQDSEIARDYIG
                     AMTLAGEYAYAGRELVVKKVLEILGNPDITFEVHNNHNFAWKEEHFGEQYWVVRKGCT
                     PAFPGQRGFIGATMSDDSVIIEGIDGELSRQALYTTVHGAGRVMSRTKSAGKRKWVKD
                     KEGTRRLSVISKGLVDFDEVKAKMKRRKIELR"
BASE COUNT          842 a          555 c          677 g          661 t
ORIGIN      
        1 gccctcgatg tttttcagtc tgttgattat ctgatagtct ttatcaaatc tgataagaat
       61 gacttcctga ttctgatgga ctgccggctt aaatattatt accggcagtg atgactgttc
      121 cgtggaaatt attctcctta tcgtggtacc catggcagct tgttttatct cctgcttgcg
      181 gtaaaagtaa agtcaattca atgcagattc tgacctccgg aactcagaat acagtctgtc
      241 tgttgcttta attaacgaca ttgttcagtc ttgactgaat actccgaacc tccttcaggc
      301 tatgcatcag ccccgtcagt cacgtcaatg atgttttccg cccgggattc ggaataatcg
      361 gttccgtttt cctgaccaca gggaaatttc tacacaagat aaaaaagata gcggaattat
      421 gatcaattag aacaggcaaa tttgaggaac aatctatgaa taaaattgtt ttaccaccgg
      481 aggagactga atttaatccg gaaagccggt acgctgttat ttaacaaatt tgattcatcc
      541 gtttaataaa agaaaattat gccaaatcca tatctgcatc gcaaagtggt tgttgtcgga
      601 gccggaaacg tcggagcaac atacagctat gcactggcac aaagtggcct ggccgatgaa
      661 atcgttctta ttgacaataa taaggatctg gtaaaaggac aggtcctcga tcttatacac
      721 ggccagccat tcttcccgac agtaaatatc cgggaaggct cccggaatga ctatgacgac
      781 gcggcagtga ttgtcgtcac cgccggctca gcacaaaagc cgggggaaac acgtcttgac
      841 ctgctgcgaa ggaatgcgga aatagtcggc agtattgccg aagatatttc agcacgtaac
      901 aaaaacgggg taatgctgat cgtaagcaat ccggtggacg taatgacctg ggtggccatt
      961 aaaagatcag gatgggaaag tggaagagta atcggctcgg gaactgtcct cgacagcgcg
     1021 cgtttcagac acctgttaag caaacactgt gatgtgaacg tgcataacgt ccatgcttat
     1081 atccttggag aacatggtga taatcagttt gccgcatggt caatgaccca tatcggggga
     1141 ataaatattg atcagtattg ccatatctgc agaaaatgcg ataactggat ggccgaacgg
     1201 gaaaagattg aacaacaggt tcgtgattca gcttatcata taatctcata taagggctca
     1261 acacatttcg ccgtggggct tgcaatggta agaatcaccg gaacaatcct caggggagaa
     1321 aacagggtaa tgaccgtatc aaccatcctc ggcggggaat tcggattaaa cgatgtctgc
     1381 ctcagtgtgc cgtcaatggt atcagacaag ggcgtcagca gaattatcga gagcccgctt
     1441 gcaaatgatg aggcaatctc cctggcagga tccgctgcaa tactgaaaga agcaataaaa
     1501 tcaatcaata tgtaaaacaa agatccctgg gaagaaatca gatcaccggc ctgtaatttt
     1561 gaagaggggg aattattaat tatattgtat cattgacgca gcttaggaga aatctttgat
     1621 gccacagggg ttgaaaaatg aaacagccac aattaaaaat tgaaaaaacc gggaatatta
     1681 caatcttcgg cagagagctg attgatgaaa attctgtcag acaaatcaaa aattgtatca
     1741 gttccgaaaa tgacatagca gttctgaatg ccgatgcaca ttacggctac ggacatccga
     1801 taggtggtgc tgttgcctac cgtgacaaga tttccctctc aggagtgggc ttcgatatag
     1861 cctgcggaaa ttacgcagtc agaaccgata tccctgtaaa atcagtcaat atagcagggg
     1921 taatggatga aatagtcaga aggatatcat tcggaatagg aaggcctaac aatgaaccgg
     1981 tcgatcaccc tgtcctggaa aagataaaca gggctgaatt cgtaccacag cgcaaactgt
     2041 cccggctggc aagggaacaa ctcggcacag taggcagcgg aaaccacttc gttgacctgt
     2101 ttgaggatga cgatggcttt ctgtggacag gtgttcattt cgggtcaagg ggattcggac
     2161 acagaacaac tatgggattc atagcaatgt caagaggcta ttcctttgat gaaaaagtca
     2221 gggagggaag tatggactcc cctccgatcc ttttcggtca ggattcggaa attgcccggg
     2281 attatatcgg tgcaatgacc cttgccggag aatatgccta cgccggcagg gagctggtgg
     2341 ttaaaaaagt acttgaaatt ctgggaaacc ctgacataac atttgaagta cataataatc
     2401 acaatttcgc ctggaaggaa gaacatttcg gggaacagta ctgggttgtc agaaagggtt
     2461 gtacaccggc atttcccgga cagaggggat tcatcggcgc aacaatgtct gacgattcgg
     2521 taattatcga gggtattgac ggtgaattgt ctcggcaggc tctgtataca acagtacacg
     2581 gcgcaggaag agtaatgtca agaacgaaat cagccggaaa acgaaaatgg gtaaaagaca
     2641 aggagggaac aagaagactc agcgtaattt caaaaggcct ggtggatttt gatgaagtga
     2701 aggcaaaaat gaaacggcga aaaattgaac ttagg
//
LOCUS       DAOSPX010000393         2724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_33814_length_2724_cov_5.656051, whole genome shotgun sequence.
ACCESSION   DAOSPX010000393 DAOSPX010000000
VERSION     DAOSPX010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33814_length_2724_cov_5.656051"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..402
                     /locus_tag="PLZ75_13590"
     CDS             214..402
                     /locus_tag="PLZ75_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73473.1"
                     /translation="MRCKTAYEKHKARARFIHQASVEPVIRHLKSDYRMPGNYLKGVE
                     GDMINTIMAVVAFNMMKR"
     gene            541..1389
                     /locus_tag="PLZ75_13595"
     CDS             541..1389
                     /locus_tag="PLZ75_13595"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73474.1"
                     /translation="MKVIEPKDLEPLSFLFRGAKGQRFAEWLIRSLAIDKVNRAYDKL
                     GAWSGSEFTTRLLKDIGVSYVVGNPERLGDLPAGAFITISNHPYGGLDGIITIDMMAR
                     IRPDYKFIVNELLSLIKTLEENFIPVVPKGDKQTTIKATSIKGIREAIEHLGAGHPLG
                     FFPSGAVSDFSLRDMKVRDRPWQESILHLIHSVKVPVLPIRFFDMNSAFFYFLGLINW
                     RVRVLRLPSEMFNKRGSQQRIGIGELISVEEQSRFPDPLSLGESLRKKVYEMPLPEKF
                     VHGNSV"
     gene            1411..2115
                     /locus_tag="PLZ75_13600"
     CDS             1411..2115
                     /locus_tag="PLZ75_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HOS73475.1"
                     /translation="MKARKNLKILFNEEVLRNVFSDRIRTNLLKRQEEMAILWLVKRI
                     PAFITSNMLTGIGFFGNMIVCASFILASFFSRYFLLLGPLGFAVSWFGDSLDGRLAYY
                     RKKPRRKYGFSLDITIDWLSIILIGFGYIVYAEGPWKMLGYGFVVLYGWEMIIALIKY
                     KITGKYLIDSGKMGPTEVRIIIGLVMIAEALIPGSLIYSAIVVVIALFFVNVLDTRKL
                     LRVADEIDKNEFIEKT"
     gene            2148..>2724
                     /locus_tag="PLZ75_13605"
     CDS             2148..>2724
                     /locus_tag="PLZ75_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73476.1"
                     /translation="MGEIIVKEVLTRKDLRDFIYLPEKVHKNEPDWLPPIYMDEWELY
                     DKKKNKSFGYADALLLIAYRDSKPVGRIMGIINRRYNEINDENHGRFCFMECYNEPDV
                     FHALISRIEQWARQNGMSKLVGPLGFSDKDPQGFQIEGFQYPLFIAAANNSPYMVELI
                     ENEGYTKKVDLVNYLGEIPRKFPDVYERVLDK"
BASE COUNT          796 a          582 c          635 g          711 t
ORIGIN      
        1 aactccgttc caaatatatg gtttttgaaa tacgaatttc atttcatttc tcatcatccg
       61 caatatggca gatattaaac ctgcatggtt cgatggttat taattagtca acctgtataa
      121 agcatctata ttactgatat ctaatgatat aagttaatgt taatatttat cagcttgcag
      181 gctttttgct agattcgtta aaaagcttgc aaaatgagat gcaaaacagc ctatgagaag
      241 cataaggcca gggcccggtt catacatcag gcatcagttg aacctgttat cagacatctc
      301 aagagtgatt accggatgcc agggaactat cttaagggag tcgaaggtga tatgataaat
      361 actattatgg cagtcgttgc ctttaatatg atgaaaagat aaaggcagat ccgtgttaca
      421 atttactttg tcctgagttt attgaccggc aactggtctg taaaaattgc cactgtacag
      481 aattattgaa aaacatgact tcttaagagt cgactattta gtaaatttgg aatttacgcc
      541 atgaaagtaa ttgagccaaa agatctcgaa ccgttgtcat tcctgttcag aggcgctaaa
      601 gggcagcgct ttgcggagtg gttgatcagg tcactggcta ttgacaaggt taacagggca
      661 tatgacaaat taggagcatg gagcgggtcc gaattcacca caagactgct taaagatata
      721 ggtgtcagct acgtggttgg taaccccgaa agacttggag atctgccggc aggcgcattt
      781 ataaccatct caaaccatcc ttacggaggg ctcgacggaa ttataaccat tgacatgatg
      841 gccaggatca ggcccgatta caaattcatt gtcaacgaac tcctctccct gataaaaacc
      901 ctcgaagaaa actttatccc cgttgtgcca aaaggtgaca aacagacaac tatcaaggca
      961 accagcataa agggcatcag ggaagccatc gaacatcttg gcgccggaca tcctctcgga
     1021 ttcttccctt cgggtgctgt ctcagacttc agcctgagag acatgaaagt aagggaccga
     1081 ccatggcagg aaagcatact ccacctgatc cactcggtga aggtgccggt gctgccaatc
     1141 aggttttttg acatgaactc ggctttcttt tacttcctcg gactgataaa ctggagggtg
     1201 cgggtgctga gactgccgtc agagatgttt aacaaaaggg gcagtcaaca gagaatcggc
     1261 atcggtgaac tgatcagcgt tgaagaacag tcgcgtttcc ccgaccctct ttccctgggt
     1321 gaatccctca gaaaaaaagt ctatgaaatg cccctgccgg aaaaattcgt acatggtaat
     1381 tcagtttaac ccagaagcaa atttaccgct atgaaagcca ggaaaaatct aaagatactt
     1441 ttcaacgaag aggttctcag gaatgtgttc agtgacagga tcaggaccaa cctcctgaag
     1501 aggcaggaag agatggcaat cttatggctg gtgaagcgaa tccccgcctt tattacctca
     1561 aacatgctta ccggtatcgg ttttttcgga aacatgattg tttgtgccag cttcatcctg
     1621 gcctcattct tcagcagata cttccttctc ctgggaccgc tggggtttgc agtaagctgg
     1681 ttcggcgatt cccttgacgg acgacttgca tactacagaa aaaaaccgag gagaaaatac
     1741 ggattttccc ttgacatcac cattgactgg ttaagcataa tactgatagg ttttggttac
     1801 attgtatatg ctgaaggccc ctggaagatg cttggatacg gttttgtcgt gctctacgga
     1861 tgggaaatga ttattgcact catcaaatat aagatcacag gcaagtattt aattgattcg
     1921 gggaaaatgg gacctacaga ggtcaggata atcatcgggc tggtaatgat cgcagaggcc
     1981 ctcattccgg gatcactcat ctactccgca attgttgtgg taattgcgct tttctttgtc
     2041 aatgtcctgg ataccaggaa attgctcaga gttgctgatg aaattgacaa aaatgaattc
     2101 atagagaaaa cttaaactat ctttatcgcc acgcaacctg actgcccatg ggagaaatta
     2161 ttgttaaaga ggtactcacg cgcaaggacc tccgtgattt tatctacctg cctgaaaaag
     2221 tccacaaaaa tgagcctgac tggctcccgc caatttatat ggatgagtgg gagctgtatg
     2281 acaaaaagaa aaacaaatca tttggttatg ctgacgccct tttgctgata gcttacaggg
     2341 acagcaagcc ggtgggccgg attatgggga tcatcaaccg caggtataac gagatcaatg
     2401 atgagaacca cggacggttc tgttttatgg agtgctataa tgagccggat gtctttcacg
     2461 ccctgatcag ccgcatcgaa cagtgggcaa ggcaaaacgg aatgtcaaaa cttgtcgggc
     2521 cactcggttt ttccgacaag gaccctcagg gattccagat cgaaggattc cagtacccct
     2581 tgttcattgc tgccgccaat aactcgccat acatggttga gctgattgag aatgaaggat
     2641 ataccaaaaa agttgacctg gtgaattacc tgggagagat accccggaag tttccggatg
     2701 tgtatgaaag ggtcctcgac aaaa
//
LOCUS       DAOSPX010000394         2719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_33903_length_2719_cov_5.258258, whole genome shotgun sequence.
ACCESSION   DAOSPX010000394 DAOSPX010000000
VERSION     DAOSPX010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33903_length_2719_cov_5.258258"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..2027)
                     /locus_tag="PLZ75_13610"
     CDS             complement(273..2027)
                     /locus_tag="PLZ75_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS73477.1"
                     /translation="MKKLIYLILIMAGTFACDMIELPESKISKEPIFNSEIGLKLYTN
                     SFYETFPNAPALYTNSYYIAVNSVIKYFTKNGFSAEESSGWNWGTLRNVNYFIVNNNN
                     ESVPLEVRNNYQGIARFFRAYFYFGMMQRFGDLPWIDHPLDVKDELLLAGRDPRTLIV
                     QKIKEDLDFAIANIKERKDNTSSTITSSVAAAFKSRVCLWEGTFRKYQTSYGLQSSAN
                     EWLQEAVDAAEYAMNSGYSIYTGDGPKLSYRKLFISSVPVSSEVLYAVTFDTDQGVVH
                     SGNRRYTSVTYGSSPAPTRSFVNTYLTLDGTPFTEIDDYQTKSFTEECQNRDYRLSQT
                     VRTPGFTRIRGGQTVRTAPNYAYAITGYHICKFTLDDEQFDNVDVCDNNAIIFRYAEV
                     LLNYAEAKAELGTLTDADWSKTIGVLRARAGITGGLDTKPTVADPYMKAKFFPDISNP
                     EILEIRRERAIELSFEGFSWPDICRWKVGDLVTNVWDGIYIPELDVPYDMDDNGTLDV
                     CFTKNLNPDKVPGVFYQYVGEKLASGATNNYQLDDATHSLVFMKDQIRTWDDKLYFYP
                     IPANDLVKNPNLGQNPGW"
     gene            complement(2039..>2719)
                     /locus_tag="PLZ75_13615"
     CDS             complement(2039..>2719)
                     /locus_tag="PLZ75_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HOS73478.1"
                     /translation="GKWLPGDIKFRDLNNDGVIDYGTNKVNDPGDRKVIGNTTPRYTY
                     GINLGVNWSNIFISGFFQGVGKQDWWPGTDNALFWGQYNRPYNNIPKSMLGNIWTVDN
                     PDAYFPRYRGYVALQSSRELAVTQTRYLQNVAYIRLKNLQVGYNLPRHIASSIRMQNA
                     RVYMSAENLWGWSPLYKITKNFDFGSIYGTDIEQAQSVPSSGIANGSQVYNYPILKSV
                     SFGVSVTF"
BASE COUNT          682 a          606 c          600 g          831 t
ORIGIN      
        1 gggatatgat ttttgtccat ggcagaatcg tattacatag taaaaatagt tatttaaaat
       61 cattccgggg aattcctgtg agtataatat tttgctttac tgttatattc cgtggaattt
      121 aggccttatt gtattgcttc ttacgggtta actgtatcag ataaccggaa ttcagtgtcc
      181 tgcctgatcc cgcaatattc tgtaaatgac ggaaaaaaga aaaggcctgt tgaaaacaag
      241 ccttttcttc ctgaaattat ttctgaagga tactaccatc ccggattctg tcccagatta
      301 ggattcttaa ccagatcgtt ggcaggtatc gggtaaaaat aaagtttatc atcccaggtc
      361 cttatctggt ctttcataaa gacaagtgag tgtgtggcat catccagctg ataattattg
      421 gtagccccgc ttgcaagttt ttctcccaca tactggtaga atacgcccgg tactttgtca
      481 ggattcaggt tctttgtaaa acatacatcc agggtgccgt tatcatccat gtcataaggc
      541 acatcaagtt ctggaatata aattccatcc cagacatttg tgaccaggtc tcccactttc
      601 cagcggcaga tatccggcca tgagaaacct tcaaatgaca gttcaatcgc tctttctcgc
      661 cttatttcca ggatttccgg attgctgata tccgggaaaa actttgcctt catatacgga
      721 tctgcgacgg tgggttttgt gtccagtccg ccggtgattc ctgcccttgc cctcagtact
      781 ccgatggttt tgctccagtc ggcatcggta agagttccca gttcagcttt tgcctcggca
      841 taattgagca gtacttcagc gtaacggaat attattgcat tattgtcgca aacatcaaca
      901 ttgtcaaact gttcgtcgtc gagtgtgaat ttacagatat ggtaacccgt gattgcatac
      961 gcatagttcg gggctgttct cactgtttgt ccgcctctga tgcgggtaaa gccgggtgtt
     1021 cggacggtct gtgacaggcg gtaatccctg ttctggcatt cctcagtgaa ggatttggtc
     1081 tggtaatcgt caatttccgt aaagggtgtg ccgtcaaggg tcagataggt atttacaaag
     1141 gaacgtgtcg gggcggggct gctgccatag gttaccgatg tatagcgcct gttgcctgaa
     1201 tgcacaacac cctggtcggt atcaaatgta actgcataca gcacttcact tgaaaccggc
     1261 acactgctta taaataattt acggtaggaa agctttggac cgtcgccggt atagattgaa
     1321 taaccggaat tcattgcata ttcagctgca tcaactgctt cctggagcca ttcgttggca
     1381 gatgactgaa gtccgtagct ggtctgatat ttccggaagg taccttccca caggcagact
     1441 ctggatttga atgctgctgc cacggaggaa gttattgttg agctggtgtt gtccttcctt
     1501 tcctttatgt tcgcaattgc aaagtccagg tcttccttta ttttttgcac aatcagggta
     1561 cgcggatccc tgcctgcaag cagtaattca tccttcacgt caagcggatg atctatccac
     1621 ggaagatcac caaacctctg catcatgccg aaataaaaat atgctctgaa aaatctggca
     1681 atcccctggt aattgttacg tacttccagt ggtacggatt cattattgtt atttactata
     1741 aagtaattaa cattgcgaag ggtaccccag ttccatccgc tgctctcctc tgcgctgaaa
     1801 ccgtttttgg taaaatattt gatgacactg tttacagcta tataatagga attggtatac
     1861 agtgctggag cattggggaa agtttcataa aatgaattgg tataaagctt caggccaatt
     1921 tcggaattga aaatcggttc cttggaaatt ttggattccg gcaactctat catgtcacag
     1981 gcaaaggtgc ctgccataat cagtattaaa tatattagtt ttttcatctt gctcattttt
     2041 aaaatgttac tgaaactcca aaactgacac ttttcaggat aggataattg tatacctggc
     2101 ttccgttggc aattccactg ctgggcactg actgggcttg ttcaatgtcg gtaccgtaaa
     2161 tgcttccgaa gtcaaagttc ttcgttattt tatacagcgg agaccagccc cataggtttt
     2221 ctgcagacat atagactctt gcgttctgca tccttatgga agaggcaata tgtctgggca
     2281 gattgtaacc aacctgcagg tttttcagac gtatgtaggc cacgttctgc agataacggg
     2341 tctgggttac tgccagttcc cttgagcttt gaagggcaac atatcccctg tatctcggga
     2401 aatatgcatc cggattgtct actgtccaga tatttccaag cattgatttg ggtatattat
     2461 tgtatggcct gttatactgg ccccagaaca gggcattgtc ggttcccggc caccagtcct
     2521 gtttgccgac accctggaaa aatccggaaa tgaaaatatt gctccagttt actcccagat
     2581 tgataccgta ggtatagcgg ggggttgtat tcccgataac tttgcggtcg cccggatcat
     2641 taaccttatt ggtaccataa tcaataaccc cgtcattatt cagatccctg aacttaatgt
     2701 cgccaggcag ccattttcc
//
LOCUS       DAOSPX010000395         2717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_33952_length_2717_cov_2.957175, whole genome shotgun sequence.
ACCESSION   DAOSPX010000395 DAOSPX010000000
VERSION     DAOSPX010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33952_length_2717_cov_2.957175"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLZ75_13620"
     CDS             complement(<1..237)
                     /locus_tag="PLZ75_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73479.1"
                     /translation="MLYGKGFSAVVFTGDMVGRSGNVEPMLALIQSIRQINSTAPIYF
                     VAGDDDPQAVVASYRGTPEVLADWVKEAQKAGAIY"
     gene            241..2592
                     /locus_tag="PLZ75_13625"
     CDS             241..2592
                     /locus_tag="PLZ75_13625"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOS73480.1"
                     /translation="MVQNEKRQGYDNQPYGFTEYLISKSLYPAGHPYSWTTIGDMEDI
                     RNATIGDVKEFYGDFYGPNNATLVLSGDFETEEAKKLIEKYFGEIKPHGQPERRTPMI
                     PAPEKTIRVYHEDNFANVPEITMVWPVPQAYHKDAWALDFLAKILASGKKSPLYRVLV
                     KEKQLTSSVNAYNSPEELAGEFMISVRANEGISLAEIENAVFEAFDRFEKEGISRRDI
                     ERVKAVSEKGFYDGLSSILGKSIRLAYYNIFPGDPGFIEKDIENIMAVKQKDVKRVYD
                     QYIKGRNYVVTSFVPREKTGLAAANSVSAEIMEENIAEAVQVEITDGGDEMTEKTESA
                     IDRSSEPPSGKEPEVNIPAVWKATLQNGIRVYGINNTELPLVNIKVTIDGGVLQDNTA
                     LPGVAGMVAAVFPQGTKTRTPEELEEETELLGSAINISAGREELVINLSTLSRNFDRT
                     MALLKEIILEPRWDESEFTIAQNRIRNRIIQSEAQPARIANIAFNKLVYGPDHIFGYN
                     PDGTRESIDRITLDDLRSWYEKNISPSVTRIHVSGNITEEQVIKALRPLESGWPVKEV
                     TFNAYPAPDVPDKSRIYFLDIPGSRQSVIYIGYPAISRDNPDFIKAEFVNYRLGGAFS
                     SILMQKLREEKGFTYGASSSFQEMKTIAPFIASSSVRSDATFESLSIFRDEMKKYREG
                     IGENEILFIRNSMIRSNALRFETNQALVNMLSTISKYGLSDDYVRQEEEIIRNMTRVE
                     HRAITNKYIIPDRMYYLVAGDAASQMKPLEKAGLGKPAILEIK"
BASE COUNT          828 a          613 c          677 g          599 t
ORIGIN      
        1 gtagatggcg ccggccttct gggcctcctt tacccaatcc gccagcacct ccggggtgcc
       61 ccggtaggag gccaccacag cctggggatc atcgtccccg gccacaaagt aaatgggtgc
      121 ggtggagttg atctgccgga tggactgaat cagcgccagc atgggctcca cattgccgct
      181 gcggcccacc atgtccccag tgaacaccac ggcggaaaag cccttgccat acagcaaaac
      241 gtggtacaga acgaaaaaag acagggctat gataatcagc cttacggatt tacggaatat
      301 ctcatctcca aaagccttta tcctgccgga catccttaca gctggaccac cataggggat
      361 atggaagata tccggaatgc aacaataggg gatgtgaagg aattttacgg ggatttctac
      421 ggacccaaca atgcaaccct tgtcctttca ggggattttg agacagagga agcaaaaaaa
      481 ctgatagaaa aatatttcgg cgaaataaag ccgcacggcc agccggaaag gagaacaccc
      541 atgatacccg ctccggaaaa gaccatccgc gtttatcacg aagacaattt tgccaatgtt
      601 ccggaaatca cgatggtatg gcctgtcccc caggcttatc acaaggatgc ctgggcgctt
      661 gattttctgg caaagatact ggcatccggg aaaaaatccc ccctctacag ggtactcgtg
      721 aaggagaaac agctgacatc ctctgtaaat gcctataaca gtccggaaga gcttgcaggt
      781 gaattcatga tttcagtaag ggcgaatgag ggaatatctc ttgccgagat tgaaaatgct
      841 gtttttgagg cttttgacag atttgaaaaa gaaggaataa gccgcaggga tattgaaaga
      901 gtaaaggctg tttcggaaaa aggtttttat gatggtctga gcagcatcct gggtaaatca
      961 ataagacttg cctattataa tatctttccg ggcgaccccg gttttattga gaaggatatt
     1021 gaaaatatca tggctgtgaa gcagaaagat gtgaaaaggg tatatgatca gtacatcaaa
     1081 ggcaggaatt atgttgtcac cagttttgta ccaagggaaa aaaccggcct tgccgccgca
     1141 aattcagttt cagcggaaat aatggaagaa aacatagctg aggcagtaca ggttgagata
     1201 actgacggcg gagacgagat gactgaaaag acagaatcag cgattgaccg cagttctgag
     1261 ccaccatcgg gaaaggaacc ggaggtgaac atacccgcgg tatggaaagc cacactgcag
     1321 aacgggatca gggtatatgg cataaacaac accgagcttc cgctggttaa tattaaagtg
     1381 acgatagacg gtggtgtgct tcaggacaat accgctctcc ccggtgttgc cggaatggtt
     1441 gctgccgttt ttccccaggg aacaaaaacc aggactcctg aagagctgga agaagaaact
     1501 gagcttctgg gttccgctat caacatttca gcaggcaggg aagaactcgt tatcaatctc
     1561 agtacccttt caagaaattt cgacaggaca atggcccttc ttaaggaaat catccttgaa
     1621 ccacggtggg atgaatcgga attcacaatt gcacagaaca ggataaggaa cagaataata
     1681 caatcggaag cccagccggc cagaatagca aatattgcat tcaataaact tgtatacggc
     1741 ccggatcata ttttcggata taatcctgac ggcaccaggg agtcaataga cagaatcacc
     1801 cttgatgatc tcaggtcgtg gtacgaaaaa aatatatccc cctcggtaac aaggatacac
     1861 gtttccggca atataactga ggaacaggtg attaaggcac tcaggccgct ggaatccgga
     1921 tggcctgtaa aagaagttac cttcaatgca tatcctgctc cggacgttcc ggacaaatcc
     1981 cggatatatt tcctggacat acccggatca aggcaatcgg ttatttacat tggctatcct
     2041 gcaatctcaa gagacaatcc tgatttcata aaggcagaat ttgtgaatta caggcttgga
     2101 ggggcatttt caagcatact gatgcagaag ctcagggaag agaaaggatt cacctatggc
     2161 gcaagcagct catttcagga aatgaagaca attgctccct tcattgcctc gtcgagcgtc
     2221 cgctctgatg ccacctttga atcactcagc attttcaggg acgaaatgaa gaaatatcgt
     2281 gaaggcatcg gggaaaatga aatccttttc atcagaaatt ccatgataag atcgaacgca
     2341 ctgcgttttg aaacaaatca ggctcttgta aacatgcttt caaccataag caaatatggt
     2401 ttaagtgatg actacgtaag acaggaggaa gagataatca gaaacatgac acgggtggaa
     2461 cacagggcca taacgaataa atacatcatc cctgacagga tgtattacct ggtggcaggc
     2521 gatgcggcca gccagatgaa gccgcttgag aaagccggat tgggaaagcc tgccatcctt
     2581 gaaattaaat gacaagagcc gcgggtcata ccggtcatcc cgctgctata ttgtaaacca
     2641 tcagggaatt tccgtggcgg acgtaaagtc tcttgtcttt tattacaaga tgagcccagt
     2701 gaggaccgga gccccgc
//
LOCUS       DAOSPX010000396         2704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_34159_length_2704_cov_4.728199, whole genome shotgun sequence.
ACCESSION   DAOSPX010000396 DAOSPX010000000
VERSION     DAOSPX010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34159_length_2704_cov_4.728199"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLZ75_13630"
     CDS             complement(<1..774)
                     /locus_tag="PLZ75_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOS73481.1"
                     /translation="MSKTYIPKEISWLSFNERVLQEAENSSTPLLERFKFLGIYSNNL
                     DEYFRVRVATLKKLSGLGAKSKEILGYNPKATLKKIQEIVLEQNSRFEKIYTSLIEEL
                     ARHNVHIVNEKELNQEQSEFVRNYFLTEVRARLMPFLIENDYKLPNLTDDAIYLAIQL
                     CNTKTAKKRFALLEIPTDVLPRFIVLPENGEDKYVIYLDDIIRFGLKDIFFIFDFDEF
                     SAYTIKLTKDSELEIADDISESYIEKVSRSLHQRKWGTPV"
     gene            complement(771..1268)
                     /locus_tag="PLZ75_13635"
     CDS             complement(771..1268)
                     /locus_tag="PLZ75_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOS73482.1"
                     /translation="MKKLIFIRHAKAEEQAAGITDFERSLTLRGKMVSHGMARRFMEK
                     EKAPGLFITSPAFRAFETALIFAGEAGVRPEDIKIDSNLYFKTDLRHFLDFLKTLPEE
                     TDSITLFGHNPSFTEIADRLSRAGCEFLTKTSVVCISFPVKSWSEVKVASGRQEYFLK
                     PVKTV"
     gene            complement(1452..>2704)
                     /locus_tag="PLZ75_13640"
     CDS             complement(1452..>2704)
                     /locus_tag="PLZ75_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS73483.1"
                     /translation="HVGAGEDDVILFTGTGMTSAIAKLQRILGLKVPEQAMKYCAFTS
                     GKYQSCKDVPSRNRPVVFLTHTEHHSNHTSWFETLADVVVLQPGPDLKVNPEALKKEI
                     IKYLDRPLLIGSFSGCSNVTGYFPPYRELARIMHEHGGYCFVDFAASAPYVEINMHPE
                     DKMQELDAVFFSPHKFLGGPGSAGVLVFNKKLYKNQIPDTPGGGTVKWTNRWGGYSYI
                     SDIEVKEDGGTPGFIQAMKTAMAIRLKERMGIKNIHNREQELIKETFRRLREIKGIHI
                     LADNITDRLGVFSFYADGIHHNLFTKILNDRYGIQVRGGCSCAGTYGHFLLDVNFSLS
                     KEITERIEAGDLSLKPGWIRLSLHPTMTDEELSFIIQAIEETMINASEWEKDYVYNSH
                     TNEFCHRNFREKESSGYSHWFEFA"
BASE COUNT          669 a          604 c          590 g          841 t
ORIGIN      
        1 gacgggtgtt ccccattttc tctgatgaag gcttctggaa accttctcaa tataactctc
       61 cgatatatcg tcagctatct ccagctccga atccttggtc agcttaattg tgtaagcgga
      121 gaattcatca aaatcgaaaa taaagaatat atccttcaga ccgaatctga taatgtcatc
      181 caggtaaata acatacttat cttcgccatt ttccggcagg acgatgaatc tcggaagcac
      241 gtccgtcggt atttcgagaa gggcaaatct ttttttggca gtttttgtat tgcaaagctg
      301 aatggccaga taaattgcat cgtcggtcag gttgggcagc ttgtaatcat tttcaataag
      361 aaagggcatc agtcttgccc tgacttctgt aagaaaataa ttccttacga attccgactg
      421 ttcctggttt agttctttct cgttgactat atgaacattg tgtctggcaa gttcttctat
      481 aagagacgta tagatttttt caaaccggct gttttgctca agaacaattt cctgaatctt
      541 tttaagggtt gctttcggat tgtatccgag aatctccttt gattttgctc ccagtcctga
      601 caactttttg agtgtcgcaa cccttaccct gaaatattca tccagattgt tggaatatat
      661 gccaaggaat ttaaatcttt caaggagggg tgtgctgctg ttctctgctt cctgcaggac
      721 acgttcattg aatgacagcc agcttatttc cttagggatg taggttttgc tcatactgtt
      781 ttaacaggtt tcagaaaata ttcctgtctg cctgaggcaa ctttcacctc tgaccaggat
      841 ttaacgggaa atgaaatgca gacaacactg gttttcgtca ggaattcaca gccggcgcgg
      901 cttagcctgt cagctatttc agtaaaagag gggttgtgtc cgaacagtgt aatactgtct
      961 gtttcttcgg gaagcgtttt cagaaaatcc aggaaatgtc tcaggtcagt tttaaaatag
     1021 agattactgt cgatttttat gtcttccggt ctgaccccgg cttcccctgc aaatatgagg
     1081 gctgtttcaa atgccctgaa ggccggactg gtaatgaaca ggccgggggc cttctctttt
     1141 tccatgaacc gccgggccat tccgtgtgat accattttac ccctgagggt gagggatctt
     1201 tcaaaatctg ttataccggc agcctgttcc tctgctttgg catgacggat aaaaatcagc
     1261 ttcttcatca gttctcagta tgtagacatg cattgataat tcatacaaat gtagttcatt
     1321 atgtgttgat ttcaactgaa gtcactgaaa aaaatgattt aggcggatga aatccctgta
     1381 cggaaggatc tgatgacagg cttttgtccc ctttacgggc tgttcagaat caatacgtgc
     1441 aaaaggactg atcaggcgaa ttcaaaccag tgagaatatc cggagctctc cttttcacgg
     1501 aagttccggt ggcagaattc atttgtgtgg ctgttgtaca cataatcctt ttcccattcg
     1561 gaagcattaa tcattgtttc ctcaatggcc tgtattataa aactgagctc ctcatccgtc
     1621 attgtcggat gaagtgacaa tctgatccat cccggcttaa gagacaggtc tcccgcttca
     1681 atcctctcgg taatttcttt tgaaaggctg aagttgacat ccaggagaaa atgtccgtaa
     1741 gtacctgcac aggagcatcc gcccctgacc tgtattccgt agcggtcatt cagaatcttg
     1801 gtgaacagat tgtggtgtat gccgtcggca tagaatgaaa aaacccccag tctgtcggtt
     1861 atgttgtctg caagtatatg tattcccttt atttccctta atctgcgaaa agtttccttt
     1921 atcagttcct gctcccggtt atggatgttc tttatcccca ttcgttcctt gagtcttatt
     1981 gccattgcag ttttcatagc ctggatgaat cccggtgtgc ccccatcttc cttgacttca
     2041 atatcactga tatagctgta gccgccccat ctgtttgtcc atttaaccgt accgccaccg
     2101 ggagtgtcgg gtatctggtt tttgtagagc tttttattga acacaagaac tcctgcactg
     2161 cctggcccgc caagaaactt atgcggtgag aagaaaacag catcaagttc ctgcatttta
     2221 tcttccggat gcatgtttat ttcaacatag ggggctgatg cggcgaagtc aacaaagcag
     2281 tatcccccgt gttcatgcat tatccttgca agttcccggt acgggggaaa atatcctgtt
     2341 acatttgaac aacctgagaa tgatcctatc agaagaggac ggtcaaggta tttgattatt
     2401 tcctttttaa gcgcttcagg atttacttta agatccggac cgggctgaag tacaaccaca
     2461 tcggcaagtg tttcaaacca tgatgtgtga tttgaatgat gttccgtatg tgtgagaaat
     2521 accacaggcc ggttccggga cgggacatcc ttgcatgact ggtattttcc ggatgtgaag
     2581 gcacaatatt tcattgcctg ttcggggacc ttcagtccga gtattctttg aagcttggct
     2641 attgctgagg tcattcccgt gccagtaaac aggataacat catcttctcc ggctcctaca
     2701 tgct
//
LOCUS       DAOSPX010000397         2663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_34871_length_2663_cov_4.476994, whole genome shotgun sequence.
ACCESSION   DAOSPX010000397 DAOSPX010000000
VERSION     DAOSPX010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34871_length_2663_cov_4.476994"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLZ75_13645"
     CDS             complement(<1..726)
                     /locus_tag="PLZ75_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73484.1"
                     /translation="MQPDRSDYEIWIADWLAGNPDSDRKKQLEDFLADNPDLKEEAES
                     LLLTSVEPGSISFPRKDLLKKSPEDFTPSQVEYLSAAYLENDLSSEQIEELNICLAKN
                     PELRLLSESVRKIKLSPPDSRYNFKRRLKKQTMAGRILKISWVGLSAAASVAILVMSS
                     VFISRIISEKKNIPETARLIQPDLISEPEMKTGKAVSEVNSQTGVSGRIIMNEGPDAD
                     IQGFISSAESRTGEGRTKPEQHVK"
     gene            complement(733..1218)
                     /locus_tag="PLZ75_13650"
     CDS             complement(733..1218)
                     /locus_tag="PLZ75_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOS73485.1"
                     /translation="MTVKEFNRAVDEYSDSLYRFILADIGDEERAGDIVQDTYEKLWR
                     NVTTLDYAVVKSWLFSTAYNGMIDIIRKERRMTVLEEKHTAELVHESQYSDLNAILHA
                     ALTRLPDNQRSVILLRDYEGYSYREIGEITGMSEAQVKINIYRGRIALKNFIGKIETV
                     L"
     gene            complement(1383..>2663)
                     /locus_tag="PLZ75_13655"
     CDS             complement(1383..>2663)
                     /locus_tag="PLZ75_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOS73486.1"
                     /translation="IRDYESLQEYINSFEFKQKQKMKPLTFRDTEEYLKLREYKNLKK
                     DPEIRSYYKAASKGEATKSAAVEKFEELKAFINSSEFLAKKKMKPFTFKDTEEYKKLL
                     EYRRLKSSSEIKDFYKFRDSREYATYRNIDGSQKLARYNELKDYVASAGFREKKNYYL
                     DKKRFEKTEMFKNIQEFNSLKKNEDIVWYFRVKDSDKFDLIKNRELIFCDEFDGDRLD
                     TTKWLTNYYWGDKLLHERYSVEGDLQAYTEKENFEVRNSLLKIITKPQKYQGKLWTPK
                     DGFRPNEFSYTSGIVNTGKSFRHQYGIFSAKVRLGNPAAKNAFWMLADKITPHIDVCR
                     TSDGKVLMDYFPSAANKFRASVASRYAKDFYIYTLEWTPDKLVWKINGTVAFTCTTDV
                     PREPMYLLFSGGLDKAINSITSMEIDWVRVYKPL"
BASE COUNT          621 a          607 c          582 g          853 t
ORIGIN      
        1 ttttacatgc tgttcaggct tcgtccgccc ttctcctgtc ctgctttccg cggaactgat
       61 aaagccctgg atatctgcat caggcccttc attcatgatt atcctgccgg atacaccggt
      121 ctggctgtta acttcagata cggctttccc ggttttcatt tctggttctg aaatcaggtc
      181 aggctgaatg agccgtgccg tttcaggaat atttttcttt tccgatataa ttcttgagat
      241 aaaaactgaa ctcattacaa gtatggcgac tgaggctgct gcacttaatc cgacccagga
      301 aattttcaga atcctgccgg ccattgtttg tttcttcagg cgccttttga aattgtaccg
      361 gctgtccggg ggactgagct ttatttttct gactgattcg gaaagaaggc ggagttcagg
      421 atttttggcc aggcaaatat tcagttcctc aatctgttca gaagagaggt cgttttcaag
      481 atatgcggca gaaagatatt caacctggga gggagtaaaa tcttcagggc tttttttaag
      541 aagatccttg cgggggaagg atatacttcc gggttcgact gaggttaaca gcagggattc
      601 tgcttcttcc ttcagatcag gattgtctgc aaggaaatcc tcaagctgct ttttccggtc
      661 actgtccgga tttcctgcaa gccagtcggc aatccatatt tcatagtctg atctgtcggg
      721 ctgcatgctt acttataata cggtttcaat ctttccgata aaatttttca gggcgattct
      781 tcccctgtaa atatttattt tgacctgggc ttcactcatg cccgttatct cgcctatttc
      841 cctgtaggaa taaccctcat aatcgcgcag caggattacg gatctctgat tgtctggcag
      901 ccgtgtcagg gcagcatgaa gaatggcgtt gagatcactg tactgggact catgaacaag
      961 ttccgccgtg tgtttttctt caagcactgt cattcttcgt tctttccgga taatgtcgat
     1021 cattccgttg tatgcggttg agaaaagcca ggatttcact acggcataat cgagcgttgt
     1081 tacatttctc cacagttttt cataagtgtc ctgaacgata tcccctgccc gttcttcatc
     1141 ccctatatca gcaaggataa agcgatacag ggaatcggaa tattcatcaa cggctctgtt
     1201 aaattccttt acggtcataa tcgggattac cttgtaaaga cgtataaaat tgctgaaagt
     1261 tacaggaaaa aataatttca cgccggctgc atccggccgg tttcccgtaa caatcccgcc
     1321 aggccgtgca tcacggctca ggacgggtat atatagaagg atttggcgcc ggttaaggcg
     1381 gattaaaggg gtttatacac cctcacccag tcaatttcca ttgaagtgat gctgttgatg
     1441 gccttgtcca gtcctccgga aaacagcaga tacatcggtt cccggggaac atcggtggtg
     1501 caggtgaatg caacagtgcc gttaatcttc catacaagct tgtccggggt ccattcaagg
     1561 gtatatatat agaaatcctt agcgtatctg gaagcgactg aggccctgaa tttattagct
     1621 gcggacggga aatagtccat cagcactttc ccgtctgatg tgcggcaaac atcgatatgc
     1681 ggggttattt tatcggccag catccagaat gcattctttg ctgccgggtt gcccagcctg
     1741 accttggctg aaaaaatgcc gtactgatgg cggaagcttt ttccggtatt gactatgccg
     1801 gaagtataac tgaattcatt cggtctgaaa ccgtctttcg gagtccataa tttcccctgg
     1861 tatttctggg gctttgtgat gatcttcaga aggctgttcc ggacctcgaa gttttccttc
     1921 tcggtatatg cctgaaggtc tccttcaacc gaatacctct catgaaggag tttgtcgccc
     1981 cagtagtaat ttgtgagcca cttggtggta tcaagcctgt ctccgtcaaa ttcatcacag
     2041 aaaataagct ccctgttttt gatcaggtcg aatttgtcgg aatcctttac cctgaaatac
     2101 catacaatgt cttcattctt cttcagggaa ttgaactcct gaatgttttt aaacatttcc
     2161 gttttttcaa atctcttctt atcgagatag tagttctttt tttccctgaa cccggcactg
     2221 gccacataat ccttaagttc attgtacctg gccagtttct gtgatccgtc aatattcctg
     2281 taagtggcat attcccttga atccctgaac ttatagaagt cctttatttc agaactgctt
     2341 ttgagtctcc tgtattcaag aagctttttg tattcctccg tatctttgaa tgtgaagggc
     2401 ttcatcttct tttttgcaag gaattcagag ctgtttataa aagccttgag ttcttcaaat
     2461 ttttcaacag cagctgattt tgttgcttct ccctttgaag cagccttgta ataggatctg
     2521 atttcaggat cctttttcag gtttttatat tcccggagct tcaggtattc ctcagtatcc
     2581 ctgaaagtaa ggggcttcat tttctgtttc tgcttgaatt caaaggaatt gatgtattcc
     2641 tgaagggatt cataatccct gat
//
LOCUS       DAOSPX010000398         2658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_34979_length_2658_cov_2.530158, whole genome shotgun sequence.
ACCESSION   DAOSPX010000398 DAOSPX010000000
VERSION     DAOSPX010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34979_length_2658_cov_2.530158"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /locus_tag="PLZ75_13660"
     CDS             complement(<1..1688)
                     /locus_tag="PLZ75_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOS73487.1"
                     /translation="MNKKTVNTREETCSCQSTGTDKIIPVVEEVVALTGKGKDRVIPI
                     LQEVQKRLSYIPSEALRHICKITDITPGQISGVSTFYSQFHHLPTGKHIIKVCSGTAC
                     HVKGSQLISDSFRRVLNIGDRKNTSPDNLFSIEEVACLGCCTLAPVVQIDDKTYGHVK
                     PTQAGDILKDFLVSGGKVSRSEDKASLAGHDAEVRIGLGSCCIAGGSRNILSRLIEIT
                     DSFDLRIKIKPVGCVGVCNQTPLLEIVAGNGEHSRYTNVTQDQVEEILLRHIGPESLT
                     KKIRYRINDLADTFVTDHLLESPVNIPADEREKYLNNFTRKQVRLATMHCGILTPDSV
                     EEYLMLGGFDALKKTIQSGDPGQITDIIIKSDLRGRGGAGFPTGEKWRIVANEPGKLK
                     YIVCNGDEGDPGAFMDRMLLESFPYRIIEGMLIAAYATGASEGIFYIRAEYPLAVERI
                     GNALKHCYEKGFLGENILGSEFSFRAEIFEGAGAFVCGEETALITSLEGRRGTPHLRP
                     PFPAVRGYRNRPTLVNNVETLAVVPWIINMGAEAFSRTGSEKSKGTKVFALAGAV"
     gene            complement(1681..>2658)
                     /locus_tag="PLZ75_13665"
     CDS             complement(1681..>2658)
                     /locus_tag="PLZ75_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS73488.1"
                     /translation="GAENTDCEVYCIDKKSGEILWSASASGIPGEPSVVPEMDYDAGM
                     AVSTAAVNASSVCAIFANGNLICLDHDGNTKWAKNLETPENVYGYSCSLIIHEDLLLV
                     QYDADIKVSLMGFELESGELKWETIRRDRPSWSSPVLANFNGTAQVIINGNPWVTGFD
                     PVSGKELWAVECMSGDVAPSVAVNGTMVYAVTDYAVLAAIRPGTGASIIWSDDMFTPD
                     VSSPVATDEYLFLATGYGDVVCYDAMKGDTLWTHYLMDQFYASPVIADDRVWLLNRSG
                     VMKVIKADSRFELLAESPLGEAADCTPAFSDGCIYIRGRENLYCISEIE"
BASE COUNT          615 a          694 c          596 g          753 t
ORIGIN      
        1 accgcgcccg caagagcaaa aaccttggtt cccttgcttt tctcgcttcc tgtcctgctg
       61 aaggcttccg ctcccatatt tataatccat ggaaccactg caagggtttc cacgttgttc
      121 acaagggtgg gccggttcct gtatcccctg acagccggga agggaggccg cagatggggt
      181 gtcccgcgtc tgccttcgag tgatgttatc aatgcggttt cctcaccaca tacaaaggca
      241 ccggcacctt caaatatctc tgccctgaag gaaaattccg aaccaaggat attttccccg
      301 agaaagcctt tctcataaca atgcttcagt gcatttccta ttctttccac tgccagcgga
      361 tattccgccc ttatatagaa tattccttca gaggcccctg tggcataggc agctataagc
      421 attccttcaa ttatccggta ggggaatgat tcaagaagca tcctgtccat gaatgcaccg
      481 ggatcaccct catcgccgtt acagactatg tatttcagtt ttcccggctc atttgccaca
      541 atacgccatt tttcacccgt ggggaatcct gcaccgccac ggcctctcaa atcgcttttt
      601 ataataatat cggttatttg tccggggtcg cccgactgaa ttgttttttt cagtgcatca
      661 aaaccgccga gcatcaggta ctcttcaaca gaatcgggag tgaggatgcc gcagtgcatg
      721 gtagccagcc tgacctgctt cctggtgaaa ttgttcaggt atttttccct ttcatcagca
      781 ggtatattaa ccgggctttc aagcaggtga tccgtgacaa aagtgtcagc cagatcattg
      841 attctgtacc ggattttctt tgtcagtgat tccgggccga tatgcctgag aagaatttcc
      901 tcaacctgat cctgagttac gtttgtatat cttgaatgtt caccgtttcc ggcaactatt
      961 tccagtaaag gggtttgatt gcatactccc acgcagccaa ccggcttgat tttgattctg
     1021 aggtcaaagc tgtcggtgat ctcaatgagc ctggaaagta tgtttctgct gccgccggcg
     1081 atgcagcatg aacccagacc gatcctgacc tcggcgtcat gaccggccag ggatgctttg
     1141 tcttctgaac gggatacctt gcctcccgaa acaagaaaat ccttcagtat atctccggcc
     1201 tgtgtcggct ttacatgtcc gtatgttttg tcatcgatct gtacaacagg cgccagtgtg
     1261 caacaaccca gacaggcaac ttcctctatt gaaaacaggt tgtccgggga agtgttcttc
     1321 cggtcaccga tgttaagaac tcttctgaat gaatcgctta tgagttgtga gcctttcacg
     1381 tgacaggctg ttccggagca aaccttaata atatgctttc ctgtgggaag atgatggaac
     1441 tggctgtaaa aggtggatac tccggatatc tgtccgggtg ttatatcagt tattttgcat
     1501 atatgcctga gtgcttctga cgggatgtaa gacagtcttt tctggacttc ctgaagtatg
     1561 gggatcaccc tgtccttccc ttttcctgtc agggcgacaa cctcctcaac aaccggaatg
     1621 attttgtctg tgccggttga ctggcaactg catgtctctt ctcttgtatt tactgtcttt
     1681 ttattcaatt tctgatatgc aatacagatt ttctcttcct cttatataaa tacacccgtc
     1741 ggaaaatgcc ggtgtgcagt cggcagcttc tcccaggggt gattcagcaa gcagctcaaa
     1801 ccttgaatcc gcttttatca ccttcatgac gccgcttctg ttaagcagcc ataccctgtc
     1861 atcggcaatc accggggaag catagaactg atccatgaga tagtgggtcc acagtgtatc
     1921 gcccttcatt gcatcataac acactacatc gccgtagcct gttgcaagga aaagatactc
     1981 atccgttgca accgggcttg atacatcggg agtgaacata tcgtccgacc atattattga
     2041 tgcccccgtc cctggcctta ttgctgcaag tactgcatag tctgttacgg catacaccat
     2101 tgtgccgttt accgcgacag aaggggcaac atcgcccgac atgcattcga ctgcccataa
     2161 ttcctttccg ctgacgggat caaagccggt gacccaggga ttgccgttta ttatcacctg
     2221 ggcggtaccg ttgaagttcg caaggacggg ggaagaccat gaaggcctgt cacggcggat
     2281 tgtttcccat ttcagttctc cgctttccag ctcaaaaccc ataagtgaaa cctttatgtc
     2341 tgcatcatac tgtacaagaa gaagatcttc atggataata agagaacatg aatagccata
     2401 tacattttca ggtgtctcca ggttctttgc ccattttgtg ttgccgtcgt ggtcaaggca
     2461 tatcagattt ccgttggcaa atattgcgca taccgagctg gcgttaactg ctgccgttga
     2521 cacggccatt cctgcatcat aatccatttc aggaactacg gaaggttctc cggggattcc
     2581 ggatgctgat gcactccaca aaatttcacc cgatttcttg tcaatgcagt aaacctcaca
     2641 atcagtgttt tctgcccc
//
LOCUS       DAOSPX010000399         2645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_35199_length_2645_cov_4.574903, whole genome shotgun sequence.
ACCESSION   DAOSPX010000399 DAOSPX010000000
VERSION     DAOSPX010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35199_length_2645_cov_4.574903"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLZ75_13670"
     CDS             complement(<1..622)
                     /locus_tag="PLZ75_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOS73489.1"
                     /translation="MDLSGKSYKAFLERREALKKQYSPKQAEKQHRKGKLTAHERIGL
                     LFDPGTFEEIDTFVTPADQPVEFGKVERTYGDGVIVGYGKVNGRLVFAYAQDFTIMGG
                     SLGVVHARKISKIQEMALKMGSPIVGMMDSGGARIQEGVASLSSYASIFHNIIQSSGI
                     IPQISIIMGPAAGGAVYSPALTDFVFMVSHTSYMFVTGPNVVKEVLN"
     gene            complement(986..2080)
                     /locus_tag="PLZ75_13675"
     CDS             complement(986..2080)
                     /locus_tag="PLZ75_13675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOS73490.1"
                     /translation="MSADLKEIFQSFTPEGTYLSGGPYGTGHIHDTYRIITAEKDRDD
                     YIVQRLNNRIFKNIAELQQNIERVTVHLRKKLEQIPGSDIKRECLRLIPSKEGKSWIK
                     DANGSYWRMYIFISNHRSYDIVDSPLKAYEGGKAIGRFQAMLADLPGEPLHDTIPFFH
                     NIEKRLETFFRTVKEDPAGRVRNVAPEIDFIMKRTEEMKIILQLGREGRIPLRITHND
                     TKFNNILLDENDRALCVIDLDTVMPGYVHYDFGDAIRTAANTAAEDEKELSKVSMDIE
                     LYKAYAEGYLSETRETLNPVEKEYLAFSPRLITYTIALRFFTDYIDGDNYFKIHHEHH
                     NLQRARAQFRLVESMEEQYGEMRKIIEKLA"
     gene            complement(2070..>2645)
                     /locus_tag="PLZ75_13680"
     CDS             complement(2070..>2645)
                     /locus_tag="PLZ75_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HOS73491.1"
                     /translation="YVGVEVIAGDTIIRYGQSIGIPMESAKYYTSLTMLGMIIGYTLG
                     IILIPKYLKQGVALRICAILGVIFSLGAILVPAEMVFSMTFIDLTTFKPIQLVLPVTV
                     LFVALLGLANSLIWPAMWPLALYGLGRFTKTAGALLIMAIAGGALIPLLYGKLADIFN
                     TQAAYWICVPCYLIVMYYAFWGYKAGLKNVS"
BASE COUNT          659 a          616 c          574 g          796 t
ORIGIN      
        1 cattcagaac ctcctttacc acgttcggcc cggtaacaaa catgtaactg gtatggctta
       61 ccataaaaac gaagtcggtg agggctgggg agtacactgc accaccggca gcaggaccca
      121 ttattatgga tatctggggt atgatgccgg aggattgtat tatgttgtga aatattgaag
      181 catagctgct gagacttgcc actccctcct gtatcctggc tcctcctgaa tccatcatgc
      241 ccacaatggg agaacccatt ttaagcgcca tctcctgtat ctttgatatt ttcctggcat
      301 gaacaacccc gagtgagccg cccataattg taaaatcctg ggcatatgca aataccagtc
      361 ttccgtttac cttgccatat cctacaatta ccccgtcccc gtaggttctt tccaccttgc
      421 cgaattccac cggctggtcg gctggtgtga caaatgtgtc tatctcttca aaggttccgg
      481 gatcgaaaag gagaccgatc ctttcatgag cagtaagctt tcctttcctg tgctgctttt
      541 cagcctgttt cgggctgtat tgttttttta atgcctcacg gcgttcgagg aaagccttgt
      601 aactcttacc cgataaatcc atttcaaata ctggtttagc gttttcccgg agcatcgtca
      661 gcaggatctg agcatttttc agtgagctcc ggattttcgg attcaaaatt agtcaatccc
      721 cttgtaattc caaacaaaac ttcgaacgca aaagtttgat tgattataag tatttaatta
      781 ttagcagtct ggaaatccaa aatattcgaa tgtaaaacaa attgcttccg ggcacccggc
      841 gggaatcctg agtctgaagc cattgacagt ctttaattct gatattctga gatatggttt
      901 gccctgcctt aacagataat tacctggatt ttttaactat acggaacggg aatgtttccg
      961 gcatatctta cctggaaaac ttgtctcagg caagcttttc gattattttt ctcatctccc
     1021 cgtattgttc ctccatgctt tcaacaagtc tgaactgtgc cctggctctc tgaaggttgt
     1081 gatgttcatg gtggatcttg aaataattgt ctccatcaat ataatctgta aaaaacctta
     1141 gtgcaatggt ataggtgatg agtcgcggcg agaaagccag atattctttt tccaccgggt
     1201 tgagtgtctc cctggtctcg gaaagatatc cttctgcata ggccttgtag agttcaatat
     1261 ccatactgac cttcgacagt tccttttcat cttcggcagc ggtatttgca gcggtccgga
     1321 ttgcatcacc aaaatcgtaa tggacatatc ccggcatcac tgtatcgagg tcaatcacac
     1381 acagggccct gtcattttcg tccagtagta tattgttgaa ttttgtatcg ttgtgtgtga
     1441 tcctgagtgg tatcctgcct tctctcccga gctgcaggat gattttcatt tcctctgttc
     1501 tcttcatgat gaaatcgatt tcgggagcga cattcctgac tcttccggca gggtcttcct
     1561 tcactgttct gaaaaaggtc tcaagccgtt tttcaatatt atgaaaaaac ggaatggtat
     1621 catgaagagg ttctccggga aggtctgcaa gcattgcctg aaaccttccg attgcctttc
     1681 cgccttcata ggccttcagg gggctgtcaa ctatatcgta ggacctgtgg ttggaaatga
     1741 agatgtacat tctccagtaa ctgccatttg cgtcttttat ccagcttttc ccttcttttg
     1801 agggaatcag ccggagacat tcccttttga tatctgaacc cggtatttgt tcgagcttct
     1861 ttcttagatg tacagtaacc ctttcgatat tttgctgaag ttcagctata tttttgaata
     1921 ttctgttgtt aagtctctga acaatgtaat catccctgtc tttttcagca gttatgatgc
     1981 ggtaggtatc atgaatatgg cctgtgccat aaggcccccc gctcaggtat gtaccttcag
     2041 gggtaaacga ctggaatatt tccttcagat cagctgacat ttttcaatcc tgctttatag
     2101 ccccagaatg cataatacat cactatcaga taacagggaa cacagatcca gtaggctgcc
     2161 tgtgtattga atatatcagc aagttttcca taaagcaatg gaatgagagc acctccggca
     2221 atcgccatta taagcagtgc ccctgcggtt ttggtgaatc tgccgagtcc gtagagtgcc
     2281 aggggccaca tggccggcca gataagtgaa tttgccaggc cgagaagggc gacaaaaagt
     2341 acggtcacag gcaatacaag ctgtatgggc ttaaatgtgg tcagatcaat aaaagtcatg
     2401 gaaaacacca tttctgccgg tacaagaatg gctccgagtg aaaagatcac cccgagtatg
     2461 gcacatatcc tgagtgcaac cccctgtttg agatatttgg ggatcagtat aatgcccagg
     2521 gtgtagccga taatcattcc aagcatggtg agagaggtgt aatactttgc agattccatg
     2581 ggtatgccta tcgactgccc gtatcttatt atggtatcac cggcaatgac ttcaactccc
     2641 acgta
//
LOCUS       DAOSPX010000400         2634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_35400_length_2634_cov_3.176037, whole genome shotgun sequence.
ACCESSION   DAOSPX010000400 DAOSPX010000000
VERSION     DAOSPX010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35400_length_2634_cov_3.176037"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..872
                     /locus_tag="PLZ75_13685"
     CDS             39..872
                     /locus_tag="PLZ75_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73492.1"
                     /translation="MNRVCYLLILVLLSLLTGTEIRADKPDISGILENLFERIIGSED
                     DTEKQRLNDSVRIIIGAYAESDSVFTHSFENLRFLGQITSPDRKLKIITWNLLLRNSP
                     NRYFSYIVRKNGKRSPNKVFTLSGMNREEPVRDDIIYTSGNWYGALYYAIQPFRNNRQ
                     LCYLVLGLDYGNIRVSRKIIDVLSFSPDDSLMLGLDCFIREEESRMREVLQYSPEGIL
                     SLRMENNKTVVFDQPVTVRTGHGDGYELSAAGVSFSGYVLHKGNWVFVADIDVKNKKK
                     K"
     gene            889..1407
                     /locus_tag="PLZ75_13690"
     CDS             889..1407
                     /locus_tag="PLZ75_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73493.1"
                     /translation="MSGTKTAHLVCYDDHRIISEDLRKRFSDPSKYRVECFHSRKDFI
                     DALGKAADTRSCRIALIVIPEKTGRQDDTGKMTEEISRRDPDLGIILIVPAGQLEETR
                     KVIKFNIDAYITKNPNAILRIHNEVKKFVSENNINIYRKRRNLSLYILAGYIIFVALL
                     VLIAYFRLPGYF"
     gene            1628..>2634
                     /gene="htpG"
                     /locus_tag="PLZ75_13695"
     CDS             1628..>2634
                     /gene="htpG"
                     /locus_tag="PLZ75_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOS73494.1"
                     /translation="MQTGKIGVTTENIFPIIKKFLYSDHEIFLRELISNAVDATQKIK
                     AMASTGDYKEELGDLTIRVELDKKKKTLKISDRGVGMTREELDKYLNQIAFSSANEFL
                     DKYKDQANNLIGHFGLGFYSSFMVSDKVEVISRSWQDGAEAVKWTCDRSPEYTIESAE
                     RDLRGTDVILHIDKESKEFLEENRIEELLKKYCKFLPVEIAFGREQEWKDGKYVDTGK
                     DKIINNTKPAWTQKPSELKEEDYQKFYRELYPIGDEPLFNIHLNVDYPFKLTGILYFP
                     KIKNNIEIQKNKIQLYSNQVFVTDSVEGIVPEFLTLLHGVLDSPDIPLNVSRSYLQSD
                     V"
BASE COUNT          824 a          515 c          611 g          684 t
ORIGIN      
        1 gcccttctgg atgactgttt gaaatactgc ggagacaaat gaacagagtc tgttatttgc
       61 tgatattggt tttgctttca ttactgaccg ggacagaaat ccgtgcagat aaaccggata
      121 tatcaggaat actggaaaat ctctttgagc ggatcatcgg atcagaagat gataccgaga
      181 agcagagact gaatgattca gtgcggatca taataggagc ctatgcggaa tcggattcgg
      241 tttttaccca cagctttgaa aaccttcgtt ttctgggcca gataacatca cccgacagga
      301 agctcaagat aataacctgg aacctgctcc tgagaaacag tccaaacaga tatttctcat
      361 acattgttcg caaaaacgga aaaagaagcc ccaacaaggt tttcaccctc agcggaatga
      421 accgggaaga gcccgtaagg gatgacatta tatatacctc cggcaactgg tacggtgcac
      481 tttactatgc aatccagcct ttcagaaaca acaggcagct ctgttacctg gttctcgggc
      541 tggattatgg gaatatccgg gtttccagaa agataatcga tgttttgagt ttttctccgg
      601 atgacagcct gatgctgggt ctggattgct ttataaggga ggaagaaagc aggatgagag
      661 aagtgcttca gtattcccct gaaggaatat tgtccctcag gatggaaaac aataaaacag
      721 tggtttttga tcagcctgtc accgtcagga caggccacgg agacggatat gaactgtcag
      781 ctgcaggggt ttctttcagc ggatatgtat tgcataaagg aaactgggta ttcgttgccg
      841 atattgatgt gaagaacaaa aagaagaaat gaactgactg aatgaattat gagcggaaca
      901 aagacagctc atctggtttg ttatgacgat caccggataa tttccgagga cctgcgtaaa
      961 aggttctccg atccttcaaa atacagggtg gaatgcttcc attcacgtaa agattttatt
     1021 gatgcccttg gtaaggcagc tgataccagg tcctgcagga ttgcactgat agttatacct
     1081 gaaaaaaccg gccggcagga cgataccgga aaaatgacgg aagagataag caggagggac
     1141 ccggatctgg ggataatact gattgtgcct gccggacaac tggaagagac aaggaaggtc
     1201 ataaaattca acatcgatgc ctatattaca aaaaatccaa atgcgatcct gagaattcac
     1261 aacgaggtta aaaagtttgt aagcgagaat aatatcaata tttacaggaa gcgccggaat
     1321 ttatccctct atattcttgc cggatacata atttttgttg cactgctggt acttatcgca
     1381 tatttcagac tgcccggtta tttttaagct tcccccttgt ttcaaacagt taagctattt
     1441 ttaaactcta ctgctttttt aaacattccg gcagttacat atactgtagt gctgacattt
     1501 aagctaagcg gtcatttttt atctctggtc cggtatcatg aatgactaat tgtcagtaaa
     1561 ccgtaatggc atatcttttg acatgcaggt gcggaagtta attgttaaat tctaaaatgt
     1621 atatattatg caaaccggaa agattggcgt aaccacagaa aacatttttc ctatcatcaa
     1681 gaaattcctt tattccgatc atgaaatttt cctgagagag ttgatttcca atgctgttga
     1741 tgcaactcag aagataaagg caatggcttc aacgggagat tacaaggaag aactggggga
     1801 tctgaccata agggttgaac ttgacaagaa gaaaaaaacc cttaaaatat ccgacagggg
     1861 agtgggcatg acccgggagg aactggataa atatcttaat cagattgcat tttcaagtgc
     1921 caatgaattc ctcgacaaat acaaggacca ggccaataac ctgataggac attttggtct
     1981 gggcttctat tcatccttca tggtatccga caaggttgaa gtgataagcc gttcgtggca
     2041 ggatggtgct gaggctgtta aatggacctg tgacagaagc cctgaataca ccatagaaag
     2101 tgctgaaagg gatctgcggg gaaccgacgt cattcttcat attgacaagg agtcaaagga
     2161 attccttgag gaaaacagga ttgaagagct tctgaagaaa tactgcaaat ttcttcccgt
     2221 tgaaatcgcg ttcggcaggg aacaggaatg gaaggacgga aaatatgtcg acacggggaa
     2281 ggacaagata ataaacaata caaaaccggc atggacacag aaaccgtcgg agctgaagga
     2341 agaagattat cagaaattct accgtgaact atatcccata ggggacgagc ccttgttcaa
     2401 catacacctg aatgtggatt atccgtttaa actgacaggt atcctgtatt ttccgaaaat
     2461 aaagaacaat attgagattc agaaaaacaa gatacagctg tattccaacc aggtttttgt
     2521 aactgattcg gtagaaggta ttgtacctga attcctcacg cttcttcacg gcgttcttga
     2581 ctcgcccgac atacccctga atgtttcgcg cagttacctt cagagcgatg tgaa
//
LOCUS       DAOSPX010000401         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_35634_length_2621_cov_3.936087, whole genome shotgun sequence.
ACCESSION   DAOSPX010000401 DAOSPX010000000
VERSION     DAOSPX010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35634_length_2621_cov_3.936087"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1785)
                     /locus_tag="PLZ75_13700"
     CDS             complement(118..1785)
                     /locus_tag="PLZ75_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOS73495.1"
                     /translation="MSVKGIFKSLFLIIFGFTRFFDIFSQGAYLPPDKPRLIVGIVVE
                     QLRYDQLEKFRNRLGENGIRRLLNEGTYYQNASFEYVITRSGPGHATIATGTEPSRHG
                     ITSDNWYLPLRDELLYCTKDISVDPVGGSYESGLHSPASLNSSTFSDELKMATNRKAR
                     VFSVGLKESSVILSAGHSADGAYWYDRNSGTWMSSTYYCRSLPGWVSDFNAANHAASY
                     LNTEWKLLRPLADYADCMPDSNNYEAGFRSVNYFPYDLKKLSTAGKNANVRDMSLLME
                     TPFGNSLTTDFALKLITEENLGSDDVTDFLAICYSATDYIGHRFGPSSYEMADAILRL
                     DREIENLMKYLNDKLGKRNILIYFTSAHGVAEIPAVLEKSRIPSGYFRQNQALQLLRS
                     YMNALYGEGDWVRGYVEKQFYLNRSLIEDARIPLEDVQKKVARFIVQFSGVATAHPYS
                     AFAPNDFGNGSFGKIINNYSPQRSGDILITLSPGWVEKHDDHVTDHSSPYDYDSHVPL
                     IWYGWTVNRQTVTRKVNMTDIAVTLSSLCRIPYPNASTGEPMFELFR"
     gene            complement(1873..>2621)
                     /locus_tag="PLZ75_13705"
     CDS             complement(1873..>2621)
                     /locus_tag="PLZ75_13705"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOS73496.1"
                     /translation="YMRDFNNGVTNGYDWYSINGGRQDFVTRELQGREVTVELHDRYI
                     TPESQLNLLWQYNYRSLLAFAENALYGIHGSVSDSETGKPVAARVFIKGYDRDSSHVY
                     SDTLTGSFVRMLDPGIYNLSFRANGYAEKEIKSVLVEEGTRTGLSVVMVRFVSPADTA
                     DSGGLTVFPNPSTDLISVILPPRQYGRINVMILNASGLKMADYFTEGHEGIPLSIDVS
                     GLKDGVYILQLRNRKSGMTDRIRFVVAGKR"
BASE COUNT          694 a          675 c          592 g          660 t
ORIGIN      
        1 agggaaatca ctgacattta cagatcgggt gcagacctga ttgttcttgg aaacggatgc
       61 gaaaaggacc cggacctgct ggctgaagcc tgtgcagcaa gagataaggc cagaaagcta
      121 cctgaatagc tcaaacatgg gctcgcccgt acttgcattg ggatagggaa tacggcagag
      181 agaagataaa gtgacagcta tgtcggtcat gttcaccttc cgggtgaccg tctgcctgtt
      241 gaccgtccac ccgtaccata tcagcggaac atgggaatca tagtcatagg gcgatgaatg
      301 atccgtgacg tggtcatcat gtttttccac ccatcccgga ctgagggtga taagaatatc
      361 acccgacctc tgcggactgt agttattgat aatcttcccg aagctgccgt ttccgaagtc
      421 attcggcgca aatgccgaat agggatgagc cgttgcaaca ccgctgaact gtacaatgaa
      481 cctggccacc ttcttctgga catcctcaag agggatcctt gcatcctcaa tcagtgacct
      541 gttaaggtag aactgctttt ccacgtatcc cctcacccag tcaccctcgc cataaagggc
      601 attcatataa ctgcgcagaa gctgcaatgc ctgattctgc ctgaaatagc ccgagggtat
      661 cctgcttttt tccagaaccg cgggtatctc ggcaacacca tgcgccgaag tgaaatagat
      721 aagaatattt ctctttccca gtttgtcatt cagatacttc atcaggttct caatctccct
      781 gtcgagcctc agaatggcat ctgccatctc atatgatgaa gggccgaacc tgtgacctat
      841 ataatcggtg gcagagtagc aaatggcaag gaaatccgta acgtcatccg atcccagatt
      901 ctcctccgtt atcagcttca gtgcaaaatc tgtggtcagg gaattgccga aaggtgtttc
      961 catcagaagc gacatatccc tgacgttggc attctttccg gcggtactca gtttcttaag
     1021 gtcgtagggg aaataattga ccgacctgaa tcctgcttca taattattcg aatccggcat
     1081 acagtcagca taatccgcaa gaggcctgag cagtttccat tcagtattca gatagctggc
     1141 agcatggttt gccgcgttga aatcactgac ccagcccggc agactgcggc agtaataggt
     1201 gctggacatc catgtcccgg agttcctgtc ataccagtat gcaccatcgg ctgaatgacc
     1261 tgcggataat atgacagaac tttccttcag tcccactgaa aaaaccctgg cctttctgtt
     1321 ggtagccatt ttcagttcgt cactgaaggt ggaagaatta agactggccg gggagtgaag
     1381 ccctgattca taacttccgc caacgggatc gacggatata tcctttgtac agtataaaag
     1441 ctcatccctc aggggaagat accagttgtc agatgttatt ccgtgccgtg aaggttcggt
     1501 gccggttgct atggtggcat ggccggggcc cgagcgggta atcacatatt caaatgacgc
     1561 attctgataa tatgtgccct cattcagaag tcttctgata ccgttctccc ccagcctgtt
     1621 ccggaacttc tcaagctgat catacctcag ctgctcaaca actataccta caataagcct
     1681 gggtttgtca ggaggcagat atgcaccctg ggaaaaaata tcaaaaaacc gtgtaaatcc
     1741 gaaaattatc agaaacagac ttttaaatat gcccttaaca ctcatatgac gaatcgagaa
     1801 taaattgaat tcacaaaagt aactttttta aagatagaat caagagcctg atgtttcagg
     1861 cggaacaggc cgctatcttt ttcctgccac aacaaacctt atccggtcag tcattcccga
     1921 cttcctgttt ctcaactgaa gaatgtaaac accgtccttc agtccggaaa catctatact
     1981 gagaggaata ccttcatgac cttcagtgaa ataatccgcc attttcagtc ccgatgcatt
     2041 aaggatcatg acattgatcc tgccgtattg cctcggtgga agtattaccg aaatcaagtc
     2101 ggtcgaggga ttggggaaaa cggtcaggcc accggaatct gccgtgtcag ccgggctgac
     2161 aaagcgtacc attacgacac tcagaccggt tctggtacct tcttcaacaa gcactgattt
     2221 aatctccttt tccgcatagc cgtttgccct gaaactcagg ttatagatac ccggatccag
     2281 catcctgaca aaacttccgg taagtgtatc ggaataaaca tgagagctgt ccctgtcata
     2341 tccctttata aacaccctgg cagccacggg cttaccggtt tcactgtcac tcacgcttcc
     2401 gtgtatcccg taaagcgcat tctccgcaaa ggcaagaagg gacctgtaat tgtactgcca
     2461 cagcagattc agctgggatt cgggtgtaat gtatctgtca tgaagctcaa cagtcacctc
     2521 gcgcccctga agctcccggg tcacaaaatc ctgcctgccc ccgtttatgg aataccagtc
     2581 gtaaccgttg gttacaccat tgttgaaatc acgcatgtaa t
//
LOCUS       DAOSPX010000402         2617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_35722_length_2617_cov_3.265418, whole genome shotgun sequence.
ACCESSION   DAOSPX010000402 DAOSPX010000000
VERSION     DAOSPX010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35722_length_2617_cov_3.265418"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLZ75_13710"
     CDS             complement(<1..345)
                     /locus_tag="PLZ75_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73497.1"
                     /translation="MMNKKSYAIISLVIILTFIAYIVYDSVKPEKNRETPEKTAAAED
                     PPDLWQAGKQLDIDYGQLMSVAVSPDGDIFLGGKSFLSRYNNSLNLVWKTSTPDGITA
                     VAVYGDTVYAASA"
     gene            complement(342..1805)
                     /locus_tag="PLZ75_13715"
     CDS             complement(342..1805)
                     /locus_tag="PLZ75_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS73498.1"
                     /translation="MGSGKRYTLIIMLTALLSGGFLVWWIADDHYPAEMVARVPGMDN
                     RPELEPRSDSVIIGEFFDTLNSFDEYVPGSWPRFRGTNFDNICRDTFNLAELWDSSGP
                     PVAWKVTLGEGYAGPAVWNGRVYVMDYNERRKADMLRCFSLKSGTELWRRWYYVDIKR
                     NHGYSRTIPAITDDYLLTIGPRSHVMCLDPLSGDLRWSLDMEKEFEIPGKTKGRITPD
                     FYTGQCPLIDDGVAVLAPGGKALMTGIDCESGQVLWTTPNPDTLRMSHCSIIPMRIHG
                     KKMYVYNAIGGVCAVSAEGADRGKLLWQTTKWNPLTVAASPVLLNDNEIAVFGSYGAG
                     GARIKISYDGSGYTASVIESHKAMEGLSSDQQTPVITGDYIWSVMPENAGPLKRQLVC
                     YHLSDLRKPVWTSGKDSRFGRGLGPYVVKEDKMFLMDDDANLFFFQLDGASASLIARY
                     DILDGIEAWGPMAIAGNYLILRDSRNMICLTIGKNSS"
     gene            2300..>2617
                     /locus_tag="PLZ75_13720"
     CDS             2300..>2617
                     /locus_tag="PLZ75_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS73499.1"
                     /translation="MNNKIYLALLASVTAISCSQKDKPLPNIVFILADDLGYGDISCY
                     GQEKFSTPNIDRLARDGMLFTQHYAGCTVSAPSRSSLMTGLHTGHTPIRGNKEWQPEG
                     QWPL"
BASE COUNT          677 a          666 c          566 g          708 t
ORIGIN      
        1 tgctgaagct gcatagaccg tatcaccgta aaccgccacg gcagtgatgc catccggtgt
       61 tgaggtcttc cagaccaggt tcagtgaatt attgtagcgc gacaggaatg attttcctcc
      121 cagaaagata tctccgtcag gcgatactgc cacagacatc agttgcccgt aatcaatgtc
      181 aagctgcttt ccggcctgcc ataggtcggg aggatcttcc gccgcagcag ttttttccgg
      241 tgtttcacgg tttttttccg gtttaacgga atcatagaca atataggcga taaaggtcag
      301 tattatcacc aatgatatta tcgcatagct ttttttgttc atcatgaaga attttttcca
      361 attgtaagac aaatcatatt tcttgaatcc ctcagtataa ggtagttgcc ggcaatggcc
      421 attggtcccc atgcctcgat accatcgaga atatcatatc tggctatcag cgatgcagag
      481 gccccgtcaa gctgaaagaa aaacaggttt gcatcatcat ccatgagaaa catcttgtct
      541 tctttcacaa catagggtcc cagaccccgt ccgaatctgc tgtcctttcc gctggtccac
      601 accggcttcc ggagatccga cagatggtaa cagacaagct gccttttcag ggggcctgca
      661 ttttcaggca tcaccgacca gatataatcc cctgtgataa cgggtgtctg ctgatcgctt
      721 gacagtccct ccatggcttt gtggctttca atcactgaag ccgtgtatcc cgaaccgtca
      781 tagctgattt ttattctggc tccccctgca ccatagctgc cgaaaacagc aatctcatta
      841 tcattcagaa gaacgggcga tgctgccacg gtgagcggat tccatttcgt ggtctgccag
      901 agcagttttc ccctgtcggc accttccgcc gacacagcgc aaacacctcc gatggcattg
      961 taaacgtaca ttttttttcc gtgtatcctc atcggaatga tggaacagtg cgacattctg
     1021 agagtatcag gattgggagt ggtccacaat acctgtccgc tttcgcagtc aatgcctgtc
     1081 atcagggctt ttccgccggg tgccagcact gcaaccccat catcaatcaa cggacactga
     1141 ccggtataaa aatccggagt gatcctgccc tttgtctttc ccggaatttc gaattccttt
     1201 tccatgtcga ggctccacct aagatctccg ctcagcggat ccaggcacat cacatgggag
     1261 cgcggaccaa ttgtcagcag gtaatcatcc gttattgcag gaatggtacg cgagtaaccg
     1321 tgatttctct ttatgtcaac ataataccat cttctccaga gttccgtccc cgatttaagg
     1381 gaaaagcatc tgagcatatc tgctttccgc cgttcgttgt aatccataac atatacccgg
     1441 ccgttccaga ctgcgggtcc ggcatatccc tcacccagtg taaccttcca ggccaccggc
     1501 ggacctgagg aatcccataa ttccgcaaga ttaaaagtgt ccctgcatat attgtcgaaa
     1561 ttggtcccgc ggaatctggg ccagcttccc ggaacatatt cgtcaaaact gttaagggta
     1621 tcaaaaaatt caccaatgat cacggaatcg gatctgggct ctagttccgg cctgttatcc
     1681 attccgggaa cacgggcgac catttctgcc ggatagtggt cgtctgcgat ccaccaaacc
     1741 aggaagccgc ctgacagaag cgctgtaagc atgataataa gcgtatatct tttccctgat
     1801 cccattaccc gtttaagcaa catcaacttt aaagccagaa cgcatcacca tttatgttat
     1861 catgaattat caggcaaaac gtaacaccat tgactgtccg gaatttgcat gcaagcccgg
     1921 aaccgggcaa tgtatatgac aagtccgcgg attccggttt tgcaggtttt gcaatataac
     1981 aaaagctgaa ttaataaact ataattatca tgattgcata tgtctgctga cggctttcag
     2041 gagtttcagg atttgaataa atatgaggct caggcaggag atgtgcagga aaattaaagc
     2101 catttcccca tataaaaggg ttttgttata atatttccct aatttatttt acttttattg
     2161 ctgacgaacc ggtaacaaaa accatcaatc cgtaaaaatg gacatagatg aatttgatga
     2221 tcttaaaata attctctggc tgatactttt tcttatcttc ggcatactga ttataacctg
     2281 attataacct gattatatta tgaacaataa aatctacctt gcacttctgg catcagtgac
     2341 ggcaatatcc tgttcccaga aggataaacc acttccgaac atcgtattta tcctggcgga
     2401 tgatcttggt tatggtgaca taagttgtta cgggcaggag aaattttcaa ccccgaatat
     2461 tgacaggctg gcccgggatg gcatgctgtt cactcagcat tatgcaggat gcaccgtatc
     2521 ggctccgtcg agatcctccc tgatgaccgg tcttcatacg ggccataccc ccatcagggg
     2581 caacaaggaa tggcagcctg agggtcagtg gcccctg
//
LOCUS       DAOSPX010000403         2602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_35982_length_2602_cov_2.767962, whole genome shotgun sequence.
ACCESSION   DAOSPX010000403 DAOSPX010000000
VERSION     DAOSPX010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35982_length_2602_cov_2.767962"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="PLZ75_13725"
     CDS             <1..1454
                     /locus_tag="PLZ75_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOS73500.1"
                     /translation="GLKQKAVSGMFWSFSDSIVTNLVQFIVGLILARLLSPSEFGLIG
                     MIAVFLAISQSLVDSSFSLALIRKKEASDIDFSTAFYFNIAAGLFFFMVLYLCAPYIS
                     SFYNQPELTEITRAVSLTIIINALGITQITRQIRNVNFRLIMKLNITGVIISGSVGII
                     LALKGYGVWSLVWRSITGSAIQTILYWVFSRWVPYFRFSKTSFRELFSFGSKLLLSGL
                     IDTIYKNIYLLVIGKFFSAAQLGYYTRAEQFTKVASQNLTISIQRVSYPILSMVQEDD
                     ERLKSGYRKLIQSTMFIAFFLLLGLAAMARPLILTLIGEQWLPCVEYLQLLCFAAMLY
                     PLQELNLNVLNVKGRTDLVLRLEVVKKTLAVPVIALGVLLGIRALLLGMIGLSVVSSF
                     INAFYSGRMIGYTIRQQLSDLIPSFILSAFVSAFVFMLSCIPGVHYALLLLIQIFVLM
                     ILVIVTGKTLQINGYIELRNIISGKLPWLKEIL"
     gene            1554..2303
                     /locus_tag="PLZ75_13730"
     CDS             1554..2303
                     /locus_tag="PLZ75_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73501.1"
                     /translation="MIKWASLLIWFIAVPAIMAQQHNYQVRRQLKTEKVKKNLKPEIQ
                     RLDSVVGFYYDTQSGSHNPVYLFDYNYRGWKANPYEVVRLILPGRTASNRQLYTYNRD
                     GSAATYIYQEWVAGSWSNVMFTRYNYSSEGNLIHEMFFRPTETGDWVSYQQHFYEYDE
                     SGNRLKYLRQMKETDGSWYDFSENFWIYDEAGRLYRRFERRIADTVVIWTETYNYGDG
                     TVPTERIRQNIKYDPVKAVIVDGRYKRSLLL"
     gene            2272..>2602
                     /locus_tag="PLZ75_13735"
     CDS             2272..>2602
                     /locus_tag="PLZ75_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73502.1"
                     /translation="MDDTRDLYYYDIFGNPNLIEQFSWTNDAWHYTGKRVLYYSFKPG
                     CYITICYEGETMTVRPEKAAYYLERGATPGECSGNEAGVVDYQGKSESFSQSPVIVYP
                     NPAYDYFH"
BASE COUNT          705 a          486 c          606 g          805 t
ORIGIN      
        1 aaggattaaa gcaaaaggct gtctccggaa tgttctggag tttttccgac agtatagtca
       61 caaaccttgt acagttcatt gtagggttga ttctggcacg attgctgtcg ccttccgagt
      121 ttggccttat tggaatgatt gctgttttcc tggctatttc acagagtctt gttgacagta
      181 gtttcagcct tgcactgata aggaagaagg aagcgagtga tattgatttc tcaactgcct
      241 tctatttcaa cattgcggcc gggttattct tcttcatggt cttatatctg tgcgctccgt
      301 atatatcatc attctataat cagccggagc ttacagaaat aaccagagcg gtgagtttaa
      361 caatcattat caatgctctg ggaataacgc agataacaag acagatcagg aatgtaaatt
      421 tcaggttgat aatgaagttg aatatcaccg gggtcatcat ttccggatct gtcggtataa
      481 tattagcatt gaaagggtat ggagtctgga gccttgtatg gagaagtatc acgggaagtg
      541 cgattcaaac gatcctttac tgggttttta gcaggtgggt gccctatttc aggttcagta
      601 aaacatcttt cagggaatta ttctcatttg gttcaaagct attgctttca ggcctgattg
      661 acactatata caagaacatt taccttcttg ttatagggaa attcttctct gctgcacaat
      721 tgggctatta tacaagggca gagcagttta cgaaggttgc ttcccaaaat ctgacaataa
      781 gcattcaacg ggtgagctat ccgatcctga gcatggtgca ggaggatgat gagcgactga
      841 agtcaggata caggaaactg atacagtcaa caatgttcat tgcctttttt cttttgcttg
      901 gtcttgcggc aatggcaaga ccccttattt taacccttat cggagaacaa tggttgcctt
      961 gcgtggagta tctccaattg ctttgtttcg ctgcaatgtt atatccgctt caggaactga
     1021 atctgaatgt cttgaatgtt aaaggccgta cagaccttgt gcttcgactt gaagtggtta
     1081 agaaaacgct tgcggttccg gtaattgctt tgggtgtctt gcttggaatc agggcactcc
     1141 ttctgggaat gatcgggtta tcagttgttt ccagtttcat caatgccttt tattctggcc
     1201 ggatgatagg gtacactatc aggcagcagt tgtctgatct cataccctca tttatccttt
     1261 cagcttttgt ttcagcattt gtttttatgc tatcttgtat tcctggtgtt cattatgccc
     1321 tgttattgct gatccagata tttgtgttaa tgattctggt gattgttacg gggaagacct
     1381 tgcagattaa cggatatatt gaactaagga acattatttc agggaaatta ccatggctta
     1441 aggagatatt atgaaatatg tttgaattaa atgatgtatg atactggtta gacggaaaaa
     1501 acgtacaatt gtttccccgt tattatccga acacagtaca aaatattcat gaaatgataa
     1561 agtgggcatc gttattaata tggtttatcg ctgttcctgc cattatggct caacagcata
     1621 actatcaggt tagaaggcaa ctgaaaacag aaaaggtaaa aaagaatctg aagcctgaaa
     1681 tccagcgtct tgattcggta gtcggttttt attatgacac ccagtctggt tctcacaacc
     1741 cggtttattt gtttgactac aattaccgtg gatggaaggc aaatccttac gaggttgtca
     1801 ggctgattct tccggggaga actgcttcca accggcaatt gtatacatat aaccgggacg
     1861 ggtcagcggc aacttatatt taccaggaat gggttgcagg gtcatggagc aatgtcatgt
     1921 tcaccagata caattattcc tctgaaggca acctgatcca tgagatgttt tttcgcccca
     1981 ctgaaacagg agactgggtt tcctatcagc agcactttta tgaatatgat gagtcgggta
     2041 ataggttgaa gtatctaagg cagatgaaag aaactgacgg cagttggtat gatttctccg
     2101 aaaatttctg gatttacgat gaagccggca ggttataccg gcgatttgag aggagaatag
     2161 cagacactgt ggtgatatgg actgaaacct ataattatgg ggatggcact gttcctacgg
     2221 agcgcatacg acagaatatt aaatatgatc ccgttaaagc agtcattgtt gatggacgat
     2281 acaagagatc tttattatta tgacattttc gggaatccga acctgataga acaatttagc
     2341 tggactaacg atgcctggca ctataccggg aaaagggttc tttattactc attcaagccc
     2401 ggctgctata ttactatctg ttatgaggga gagacaatga cagtccgtcc ggaaaaggct
     2461 gcatattatc ttgagcgggg tgctacacct ggtgagtgtt ccgggaatga agccggcgtt
     2521 gtagactacc agggaaaatc tgagagtttc tcacaatcgc ctgtaattgt ataccctaat
     2581 ccggcttatg attattttca cg
//
LOCUS       DAOSPX010000404         2592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_36149_length_2592_cov_4.808829, whole genome shotgun sequence.
ACCESSION   DAOSPX010000404 DAOSPX010000000
VERSION     DAOSPX010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36149_length_2592_cov_4.808829"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="PLZ75_13740"
     CDS             complement(<1..1636)
                     /locus_tag="PLZ75_13740"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOS73503.1"
                     /translation="MTVTSVSTDSHVTIDGYLDEASWQNATLITGFTQYEPLEGAESD
                     FKTEVRVLFGKDDLYIGAMLYDSRENIENNLGRRDEYNRADWFLVSIDSYYSGRTAFT
                     FAVNAAGVQLDGLQDDGRKVSSSEINPLLPPGLDVSWDAIWFSGVRISDEGWTAEMRI
                     PYNMLRYKRKDPQTWGIYFLRRIPNSGEVSEWPYIPRYRRNNLVSAYGEIAGIHGIEP
                     RRNIQIRPYILTGFGISENENRPGTAAYNFKYNAGGDIKIGIGPNVILDATIRPDFGQ
                     IEADPAVLNLTAFETLFPEKRPFFLEGADIYKFGIGFSRLYYSKRFGADEPIIAAMKL
                     SGRTVKDLSFGLLGVTAGQGFSPVNNYALLRATQRIGNYSSAGGILTYYYSPAGRGRG
                     WQSMTGGIDWDLRFKNNRYGFEGILAFSDRNSLVPGKDDQKGMMNSLVIRKRQGDIDG
                     HVAFLMFTDQYNPNDMGWISFEQNWYQIWANGKYKIRGGKPFGKFQRGEIISDYSRRL
                     SILEHYDMGSEVYIKPSFITNRFRQIKFGTTFFDLLG"
     gene            complement(1811..>2592)
                     /locus_tag="PLZ75_13745"
     CDS             complement(1811..>2592)
                     /locus_tag="PLZ75_13745"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOS73504.1"
                     /translation="NPRGELITLPCISAYVGADVVSGLAVIKPVHRKYLFIDIGTNGE
                     MALVDGNRIYACATAAGPAFEGGSISCGMAAVNGAVSYFAGPEDYRVIGNTEPAGICG
                     SGIVDIVAYLLNNNIIDSTGLLKSPFVVYPFNRIQVTQQDIREIQLAKSAIYSGIKVL
                     LKSSGMNFEDISALYLAGGFGNYINTGSAVRTGLLPSGLGNRIYAIGNSAGIGTLQYL
                     KSEDFAEKLDRIVSVTEYIELSDSDDFITEFVMNMDFRENG"
BASE COUNT          683 a          657 c          533 g          719 t
ORIGIN      
        1 ccccgagcag atcaaaaaac gtggttccga atttaatctg cctgaacctg ttggtgatga
       61 aactcggctt gatgtagact tcacttccca tgtcataatg ttcaagaatg gaaagccgcc
      121 ttgaataatc actgataatc tctcccctct gaaatttacc gaatggtttt cctcccctga
      181 tcttgtattt tccgttggcc catatctggt accagttctg ttcaaagctt atccatccca
      241 tgtcattggg attatactgg tccgtgaaca tcaggaaggc cacatgtccg tcaatatctc
      301 cctgtctttt cctgatcacc aggctgttca tcattccttt ctggtcatcc tttcccggaa
      361 caagggaatt cctgtcagaa aatgcaagga tcccttcgaa gccatacctg ttattcttga
      421 accgcagatc ccagtcaatt cctcccgtca tgctctgcca tcccctgccc ctgccggcag
      481 gactgtaata ataagtcagg attccaccgg ccgatgaata atttcctatc ctctgggtgg
      541 cacggagcag ggcatagttg tttacaggac tgaaaccctg tccggcagtt actcccagaa
      601 ggccgaaaga caagtctttt acggtccggc ctgaaagttt cattgctgct ataatgggct
      661 catcggcgcc aaaacgcttg gaatagtata gtctgctgaa tcctatgccg aacttgtaaa
      721 tatcggcacc ttccaggaaa aagggccgtt tttcaggaaa aagggtttca aaggcagtaa
      781 ggttgagaac agccggatct gcttcaatct gtccgaaatc aggtctgatg gtagcgtcaa
      841 ggattacatt cggaccgata ccgatcttga tatcgcctcc tgcattatac ttgaaattat
      901 aagctgctgt ccccggcctg ttctcatttt cggatatacc gaagcctgta agaatatatg
      961 gcctgatctg tatattccgt ctgggttcta taccatggat tcctgctatt tcgccatagg
     1021 ccgacacaag attatttctc ctgtaccgtg gtatataggg ccattcggat acttcaccgg
     1081 aattcgggat ccgccggaga aaataaatac cccaggtctg aggatctttc cttttgtatc
     1141 tgagcatatt gtaggggata cgcatttcag ctgtccagcc ctcatcgctg atcctgaccc
     1201 cggaaaacca tatggcatcc catgatacat ccagacccgg cggaagcagg ggattgattt
     1261 cactggaact gacttttctg ccgtcatcct gcaggccgtc aagctggact cccgcagcat
     1321 tcacggcaaa agtgaatgca gtcctgccgc tgtaatagga atctattgat acaagaaacc
     1381 agtcggccct gttatattca tcccttcgtc caagattatt ctcaatgttt tccctggaat
     1441 cataaagcat ggctccgata tacagatcat cctttccgaa caatacacga acctctgttt
     1501 taaaatctga ttcagcacct tccagtggtt catactgggt gaagccggtt attaacgtgg
     1561 cattctgcca tgaggcctca tccagatatc cgtcaatcgt gacatgggaa tctgttgaaa
     1621 cagaagtgac cgtcatatcc tgctgtgact gcagttcagc agaaaaaaac aggatgctga
     1681 aaatgaaagt aaggaacagt ggtttatcca tacaagaaag tcatacgatc tattgttaat
     1741 ctcctcctga acgaacctgt tttccaggaa ccggatttgt aatctccaac aatatacact
     1801 ttttccggaa tcagccgttc tccctgaaat ccatattcat aacaaattct gttataaaat
     1861 catctgaatc ggaaagctct atatattcgg taactgatac tatcctgtcc agtttttccg
     1921 caaagtcttc cgatttcaga tactgcagag ttccgatacc tgcgctgttt cctatggcat
     1981 aaatcctgtt cccgagcccg gaaggcaata atccggtcct tacggcagaa ccggtattta
     2041 tgtaattgcc aaatccgccg gcaagataaa gcgcacttat atcttcaaag ttcatcccgg
     2101 aacttttaag taaaactttt attccggaat aaatggctga ttttgccagc tggatctccc
     2161 gtatatcctg ctgggtaacc tgtatcctgt taaagggata aacaacaaag ggggatttta
     2221 acagtcctgt actatcaata atattgttat taagaagata tgccacaata tcaacgattc
     2281 cggatccgca tattccggca ggctcagtat tgcctataac ccggtagtct tccggcccgg
     2341 caaaatatga taccgcaccg ttaaccgcag ccattccgca tgaaatactg ccgccttcaa
     2401 atgccggtcc ggctgcagtg gcacatgcat atatccgatt gccgtcaaca agggccattt
     2461 ccccgtttgt tccgatatca ataaaaagat atttccggtg aacaggtttt atcactgcca
     2521 gtccgcttac cacatcggct ccgacatacg ctgaaataca aggcagggtg ataagttctc
     2581 cgcgaggatt ga
//
LOCUS       DAOSPX010000405         2563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_36734_length_2563_cov_3.110447, whole genome shotgun sequence.
ACCESSION   DAOSPX010000405 DAOSPX010000000
VERSION     DAOSPX010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36734_length_2563_cov_3.110447"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLZ75_13750"
     CDS             complement(<1..636)
                     /locus_tag="PLZ75_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73505.1"
                     /translation="MKKNSFVQYCRISLLALVFVSFSGICTAPALGYRARMDNFRKLS
                     EYVRREHYKSEFARFIKDLGKSESGNNWQCVNLIGCFGEWQFAEKTLHYLGHKHITAR
                     KFKKDPSIFPPDLQLQALKSLIRVNLVSLKDYERFAGDTIRGVVITRSGMIAASHLGG
                     ARSLKLFLNSKGRINKADVLGTSISDYLWKFRNYDLDTFDRYDLYCDASAHA"
     gene            complement(1073..>2563)
                     /locus_tag="PLZ75_13755"
     CDS             complement(1073..>2563)
                     /locus_tag="PLZ75_13755"
                     /inference="COORDINATES: protein motif:HMM:NF019157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HOS73506.1"
                     /translation="LLLLIIIPGITDAQKEVIKDKCIPCEKLTELRLPDVKITEAEIV
                     AEGSSYCKVLGIIGKEINFEILLPDDWNGIYLMGGGGGFVGTIQNAVRSSVENGYATS
                     GTDTGHDAKISPGAGWALDNMERQLNFGYLAVHRTAEVSKAIIASYYGSGPEYSYFMG
                     CSRGGGQAMMEAQRFPEDFDGIVAAAPAFNWPALAAEFIQNTRAAFPEKLTEPLFMPE
                     HIRILHYAVLEQCDQIDGVKDSIISNPERCRFDFEVLPKCRNGKAGKECFTEVQINSL
                     KKIYDGIDLGNGIAYPGFPFGGEGEAGGWTSWITGSGTAVTSTEYPSSQAYFGIEVFR
                     YLILQNPDWDYTEYDFRGYDREMKYASSYLDATSDDYSGFRDRNGKIILWHGWNDPAL
                     SARATIDHYNAVKEKDPAVNSYMRLYLLPGVLHCGGGKGPSETDWITLIRDWVEKDIA
                     PGKIIVRKSVSGRAMMSRPVFPYPAEAVYDGRGNPYRESSFTVRQP"
BASE COUNT          688 a          635 c          522 g          718 t
ORIGIN      
        1 agcatgggct gacgcgtcac aatagagatc ataacggtca aaggtatcca gatcgtaatt
       61 cctgaacttc caaaggtagt cggatatgga agtaccgaga acatcagcct tgttaatcct
      121 tccttttgaa ttcagaaaca gcttgagtga tctggctccg cccaggtggg atgctgcaat
      181 cattccggac ctggtgatga caactcctct tattgtgtct ccggcaaatc tctcataatc
      241 ctttagtgag acaagattca ccctgatcag cgatttcaat gcctgaagct ggagatcggg
      301 cggaaaaatc gaaggatcct tcttaaactt gcgcgccgtt atgtgcttat gaccaagata
      361 gtgcaatgtc ttttcagcaa actgccattc accgaaacag cctatcaggt taacgcattg
      421 ccagttattg ccggactcgc ttttcccgag atcttttatg aatctggcaa attcagattt
      481 ataatgctct ctcctgacat attccgaaag tttccggaaa ttatccattc tggcacggta
      541 acctaaggcc ggtgcagtac atattccgga aaaggaaaca aataccagtg ccagtaatga
      601 aattctgcaa tattgaacaa atgaattctt tttcatctta tcacagttta atatcacagt
      661 gtaaaaatac tgcaaggaaa agggcttgtc aatacgctaa tgcccgtaca gtgggctttt
      721 aaagcattct caccctgtcc tttgtcaaat tattacagca gaagacttgt caccggcagg
      781 tatttaccac tgattaaggc accttaatca aattctttat gatattgact tgagaagtac
      841 ggtaaattca gttcaatcat attatcccca atataatcaa actgatattc aaggtgttaa
      901 tcctgaattt agctgataat tttgttgcat tgcactttat ccccaaattg acgaacaaca
      961 gcggaaaact gattaaactc tatgaaatac ctcattttta aggaaataat ttgcaacagg
     1021 atgctgtgaa aacgtcagat ccggtaaaaa tgcctccgga tttgtttgac acttaaggct
     1081 gcctgacagt gaaactgctc tccctgtaag gattcccccg gccgtcataa acggcttccg
     1141 ccggatacgg aaaaaccggt cttgacatca ttgccctgcc gctgacactt tttctgacga
     1201 tgattttccc gggagcaata tctttttcaa cccagtcacg gataagggtg atccagtctg
     1261 tctccgaggg cccctttcct cccccgcagt gaaggacgcc cggaaggagg taaagtctca
     1321 tataactgtt taccgccgga tctttttcct ttactgcatt gtagtgatca atcgttgcac
     1381 gggcagaaag agccggatca ttccagccgt gccagaggat tatttttccg ttcctgtccc
     1441 tgaatccgct gtaatcgtca gatgtggcat caagatatga cgacgcatac ttcatttccc
     1501 tgtcatagcc cctgaagtca tattcagtat agtcccagtc cgggttctgc agaataaggt
     1561 agcggaaaac ttctatgcca aagtaggcct gggaactcgg atattctgtg ctagtaacag
     1621 cagttccgct gcccgttatc cagcttgtcc agcctccggc ctcgccttcc ccgccaaaag
     1681 gaaaaccggg ataggcaata ccatttccga gatcaatgcc gtcatatatc tttttaagcg
     1741 aattaatctg aacctcagta aaacattctt tccctgcctt gccattcctg cacttcggaa
     1801 gaacctcaaa atcaaatctg cagcgttcag gattactgat aatgctgtct tttacaccgt
     1861 caatctgatc acattgttcg agaacagcat aatggagtat ccggatatgt tccggcataa
     1921 aaagcggttc tgtcagtttc tccgggaaag ccgcacgggt gttctgaatg aattcagcag
     1981 ccagggccgg ccagttgaaa gccggtgcgg ctgcaacaat cccgtcaaaa tcctccggaa
     2041 agcgctgggc ttccatcatt gcctgacctc ctcccctgga acatcccata aaatatgagt
     2101 attccggacc gcttccgtaa tagcttgcaa ttattgcctt tgatacttcg gcggtcctgt
     2161 gaacagccag atatccgaag ttcagctgcc tttccatgtt atcaagagcc catccggctc
     2221 caggtgatat ttttgcatca tgacccgtat cagtgccgga tgttgcatac ccgttttcaa
     2281 cagaagaacg gacagcattc tgaattgtcc ctacaaatcc gcctccgccg cccatcaggt
     2341 agattccgtt ccagtcatcc ggaagaagga tttcaaaatt gatttccttc cctataatgc
     2401 ccagtacttt acaataagat gatccttctg ctacaatttc agcttcagtt attttaacat
     2461 caggaagtct cagttctgta agtttttcac agggaatgca cttgtccttt atgacttcct
     2521 tctgagcgtc agttatgccc gggattatga ttaacaacag cag
//
LOCUS       DAOSPX010000406         2549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_36992_length_2549_cov_3.419407, whole genome shotgun sequence.
ACCESSION   DAOSPX010000406 DAOSPX010000000
VERSION     DAOSPX010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36992_length_2549_cov_3.419407"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLZ75_13760"
     CDS             <1..985
                     /locus_tag="PLZ75_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOS73507.1"
                     /translation="QAEDEIAGMCAALGAAFAGSLAVTSTSGPGLALKSEAIGLAVMA
                     ELPVVIVNVQRGGPSTGLPTKTEQADLMQALYGRNGESPVVVLAAGSPSDCFRFAYEA
                     ARIALEHMTPVILLTDGYLANGSEPWKIPEMKDLPPIQPPFASQDEQPFMPYRRNSST
                     LVRSWAVPGTAGLEHRIGGLEKTPEGIVSYSPENHESMVRLRAGKIARVLEKVPDLQV
                     YGEDSGDLLLAGWGGSGGYIVSAVKELQSEGFSVSFANFNYINPLPGNVREVFSRFRK
                     IAVAELNTGQFAAWLRSQCGGFGYEQINKVQGLPFTTKEIREKCLKILEDI"
     gene            998..2014
                     /locus_tag="PLZ75_13765"
     CDS             998..2014
                     /locus_tag="PLZ75_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOS73508.1"
                     /translation="MDSYQYTAEDLKSPQEVRWCPGCGNHAVLNAVRKALIELGMPRE
                     QYVFVSGIGCSSRFPYYMNTYGFHGIHGRAAAIASGICIANPGLSVWQITGDGDALAI
                     GGNHFIHVIRRNINMNILLFNNQIYGMTKGQYSPTSEKGLVTRTSPFGTIEKPFSVGE
                     LVMGGQGTFFARTADYNMSLSTGIYVEAARHRGTSVVEVLQNCVIFNDGIHDVVTGRE
                     VRDERTIVLRHGQPMIFGKEGDKGLVLDGYRLKVVKIGENGISAGDILVHDKKEADPA
                     IHYLLAAMRYPEYPVALGVIRSVEGKTYEDGLEEQITDIRKGAEIKSADDLLNSGPVW
                     ETGH"
     gene            2037..>2549
                     /locus_tag="PLZ75_13770"
     CDS             2037..>2549
                     /locus_tag="PLZ75_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS73509.1"
                     /translation="MIDNIDELGKYDFSDTSFDLLMQKRIHRVLIICSNYDNYMLEED
                     GRIDEQIFNEYVSLSLRHPPVFVQTDNAEDALRILREESIDLVISMESLKGTDVFALA
                     KRIKNEYPDIPIVVLTYLSREVTLRLKGEDMSAIDYVFCWLGDASVILAIIKLIEDKM
                     NGEHDIEVIGV"
BASE COUNT          671 a          569 c          729 g          580 t
ORIGIN      
        1 tcaggctgag gatgaaattg ccggcatgtg tgcagccctt ggtgccgctt ttgccggaag
       61 ccttgcagta acttccacat cgggccccgg cctggctcta aaatccgaag ccatcggact
      121 ggcagtaatg gcagaattgc cggtggtcat tgtcaatgta caaagaggag gcccctccac
      181 cggcctgcct accaagaccg aacaggctga cctgatgcag gccctgtacg gaagaaacgg
      241 tgagagcccg gtggttgtcc tggctgcagg cagtccctcc gactgcttcc ggtttgccta
      301 tgaggctgca agaattgccc tggagcacat gacacctgta atactcctca ccgacggtta
      361 cctggcaaat ggctcggaac cctggaaaat acctgagatg aaggatcttc cgccaataca
      421 gccgcctttt gcctcacagg atgaacagcc tttcatgcca taccggcgga acagttccac
      481 cctggtccgt tcctgggccg ttccgggtac agccggactg gaacaccgta tcggcggtct
      541 ggaaaagact cctgaaggga tagtatccta ttcaccggaa aaccatgaat caatggtaag
      601 actaagggcg ggcaaaattg cccgggttct ggagaaagta cctgatctgc aggtatacgg
      661 cgaggattcg ggagatctcc ttcttgcagg ctggggcgga tcggggggat acatagtttc
      721 ggcagtaaag gagctgcagt cagaaggctt cagcgtcagc tttgccaatt tcaattacat
      781 aaatcccctt ccgggaaatg taagggaggt tttcagcaga ttccggaaaa ttgcggtggc
      841 agagctgaat acaggacagt ttgcagcctg gctgaggtcg caatgcgggg gattcggcta
      901 tgaacagata aacaaggttc agggattgcc gttcaccact aaggaaatca gggaaaaatg
      961 tcttaaaata ctggaggata tttagataat ggcagcattg gattcatatc agtatacagc
     1021 ggaggatctg aaaagtcccc aggaagtcag atggtgcccg ggatgcggta accatgccgt
     1081 cctgaatgca gtccggaaag ccctcatcga gctgggcatg ccaagggaac aatatgtttt
     1141 tgtttccggc ataggctgtt cttccaggtt cccctattac atgaatacct acgggtttca
     1201 cgggatacac ggccgtgcgg ctgccattgc ctcaggaatc tgcatagcaa atcccggcct
     1261 gtcggtatgg cagatcacgg gtgacggcga cgcacttgca atcggcggca atcattttat
     1321 tcacgtgatc cgcaggaata tcaatatgaa catactattg ttcaacaatc agatttatgg
     1381 catgaccaag gggcagtatt cgcccacttc cgaaaagggc ctggttaccc ggacctcacc
     1441 cttcggtacc attgaaaaac ctttttccgt cggggagctt gtgatgggcg ggcagggcac
     1501 tttttttgcc agaacggcag attacaacat gagcctttca acggggattt atgttgaggc
     1561 tgccaggcac aggggaacgt cggttgtgga agtgcttcag aactgtgtga tcttcaacga
     1621 cggcattcat gacgtggtga caggcaggga agtcagggat gagagaacga tagtgctcag
     1681 gcacgggcaa cctatgattt tcggtaagga aggtgacaag gggctggtgc ttgatggtta
     1741 caggctgaag gttgtgaaaa taggtgagaa tggcatttct gccggtgata tactggttca
     1801 tgataagaag gaagcggatc ccgccatcca ttacctgctg gcagccatga gatatcctga
     1861 atatccggta gccctggggg taatcagatc cgtggaaggg aaaacctatg aagacgggct
     1921 ggaggagcag atcacggaca tcaggaaagg agcggaaata aaatcggcgg acgatctgct
     1981 caacagcggg ccggtatggg aaacagggca ttaatcagta tattaagccc atgacaatga
     2041 tagacaatat agacgagctt ggaaaatatg acttcagcga tacctccttt gatctgctga
     2101 tgcagaagag aatacacagg gtgttgataa tatgcagtaa ttatgacaat tacatgctgg
     2161 aggaggacgg gaggattgat gagcagattt ttaatgagta cgtgtcgctg agcctgcgcc
     2221 atcccccggt ttttgtacag acagacaacg cggaagatgc actcagaata ctcagggaag
     2281 aaagcattga cctggtcatt tcaatggaaa gcctgaaggg gacggatgtt tttgcactgg
     2341 caaaaagaat aaagaatgaa tatccggata ttcccattgt tgttctgacc tatctgtcaa
     2401 gggaggtaac cctgcgtctc aagggcgagg acatgagtgc catagactat gtgttctgct
     2461 ggctggggga tgcttcggtg atacttgcaa taatcaagct tatcgaggat aagatgaatg
     2521 gcgaacacga tattgaggtt atcggcgtt
//
LOCUS       DAOSPX010000407         2540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_37175_length_2540_cov_4.347284, whole genome shotgun sequence.
ACCESSION   DAOSPX010000407 DAOSPX010000000
VERSION     DAOSPX010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37175_length_2540_cov_4.347284"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..75
                     /locus_tag="PLZ75_13775"
     tRNA            1..75
                     /locus_tag="PLZ75_13775"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Val,seq:tac)
     gene            complement(235..1581)
                     /locus_tag="PLZ75_13780"
     CDS             complement(235..1581)
                     /locus_tag="PLZ75_13780"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS73510.1"
                     /translation="MKFRFLLTVLALLSASLFISAQQAANKPEREEWFMDMGFGMFIH
                     WSVDSQVGAVISHSMAGASEDYLARFENDLPATFNPRKFDPDEWAVLAKLAGMEYVVF
                     TSKHHSGFCMWDTKTTEFNIMNTPFRRDAMREILDAFRRRGIATGLYFSPEDFHYFRK
                     NNIPVGRLQDPLHYPANNPGLMAYNKAQIRELLTGYGKIDIFFIDGPEEGLKEYAWQL
                     QPDIVVTRGQMQTPEQQLPDRPLPGPWEACFTMGTDWQYKPTNDPHKSGTEIINMLIE
                     TRAKGGNLLLNIGPKPNGEIQIEQEALLREIALWNLVNGEGIHNIRPWEITREGDIWF
                     TRGKDNSVYAFVPGGDNWKYGERREFVLKTLSGTEKTRISILGYSSELVEYRPGYDAK
                     IYFSSTPVGLVVSAVNGHRLYTNNRWPNPVVLKLENVRFREIDSTEGRRSEIDGAL"
     gene            1651..2460
                     /gene="proC"
                     /locus_tag="PLZ75_13785"
     CDS             1651..2460
                     /gene="proC"
                     /locus_tag="PLZ75_13785"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOS73511.1"
                     /translation="MGKDKIAVLGGGNIGTSLAKGLIKSGQFTAEEIIVTDKRGSRIS
                     YLRENGFRVTENNGEAIDHARIIIMAVKPQQFKTLADEIKSHLTSGHILISTITGISH
                     DNIEAAFGPLPNVRIMPNTAIEICESMTCMSFRNMKKDQKEYISSLFDKMGKTIPVPE
                     ELMDAATVIGACGIAFALRFMRAMSQGGIEIGFNAEMSQMITAQTVRGAARLIMESGN
                     HPEKEIDKVTTPQGITISGLNEMEHQGLSSAVIKGLTVSFRKLENLSSSGK"
BASE COUNT          668 a          606 c          571 g          695 t
ORIGIN      
        1 gggcgattag ctcagttggt tcagagcata tgccttacaa gcatagggtc gggggttcga
       61 ctccctcatc gcccacaacc attcactccc gtggcatccc gatgaggtat cttccatacc
      121 ctgatgaggt ttcctctata tcctgataat attctttgca catatctgaa aatcaggctg
      181 tttaatatct gattttttta ttgccggcag acttgtaagg agatagagtt ttttttaaag
      241 ggcgccgtcg atttccgatc tcctgccctc tgtactgtct atttcccgga acctgacgtt
      301 ttcaagcttc agcacaacag gattgggcca tctgttgttc gtatagaggc ggtgtccgtt
      361 tacggcactt acaaccaggc ctaccggagt ggaactgaaa tagatttttg catcatatcc
      421 cggcctgtac tcaaccagtt cggatgaata tcccagaatg cttattctcg ttttctcagt
      481 gcctgacagg gttttcagca caaattccct gcgttccccg tatttccagt tgtcaccgcc
      541 gggaacaaag gcatatacgc tattatcctt ccccctggta aaccagatat ccccttccct
      601 ggttatttcc catggccgga tattgtgaat accttcaccg tttacaagat tccacagggc
      661 gatttcccgc agaagggctt cctgttcaat ctggatctcc ccgttgggtt tgggtccgat
      721 gttcagtaaa aggttccctc cctttgccct ggtttcaata agcatattga ttatttcagt
      781 tccgcttttg tgaggatcat ttgtaggttt atactgccag tctgtaccca ttgtaaaaca
      841 ggcttcccac ggtccgggca agggcctgtc aggcagctgt tgctccggtg tctgcatctg
      901 tccgcgtgtt accacaatat ctggctgcaa ctgccaggca tattccttca ggccttcttc
      961 gggcccgtca atgaaaaata tgtctatctt cccgtaaccc gtaagcaatt ccctgatctg
     1021 ggccttgtta taagccatca gtccgggatt gtttgccgga taatgcagtg gatcctgcag
     1081 acgtccgacc ggaatattgt ttttccggaa atagtggaaa tcctcggggg aaaaatataa
     1141 tcccgtggca attcccctcc tgcggaatgc atcaagtatc tccctcattg catcccgtct
     1201 gaagggagtg ttcatgatat tgaactccgt cgtttttgtg tcccacatgc aaaaacccga
     1261 atgatgcttt gatgtaaaaa caacatattc cattccggca agttttgcca gaacagccca
     1321 ttcatcggga tcaaatttcc gcggattgaa agtagccgga agatcatttt caaatctggc
     1381 aagataatct tcagatgcgc ctgccattga atgacttatt acagctccga cctgggaatc
     1441 cacgctccag tgaataaaca ttccgaaccc catgtccatg aaccattctt cccgttcagg
     1501 cttattggct gcctgctgtg cggaaataaa aaggcttgct gataataaag ccagaaccgt
     1561 aagtagaaat ctgaatttca taaaacattg tttttaattc cggagggtaa atatagtttt
     1621 taattatctt cgctttctta tttcagaaaa atgggaaaag ataagattgc tgtcctcgga
     1681 ggaggaaata tcggcacttc acttgcgaag ggacttataa aatcaggcca gtttacagct
     1741 gaagagatta ttgtgaccga caaacgcggg tcaagaatct catacctccg ggagaacgga
     1801 ttcagggtaa cagaaaacaa tggcgaggcc attgaccatg cccgcattat tatcatggct
     1861 gtaaagccac agcagtttaa aacactggcg gatgagataa aaagccatct cacttccggt
     1921 catattctga tctctaccat tacgggaata agccatgata atattgaggc ggccttcggc
     1981 cctctgccca atgtcagaat aatgccgaat acagcaattg agatctgcga gtcaatgacc
     2041 tgtatgtctt tcaggaacat gaaaaaagat cagaaggaat atatttcctc cctgtttgat
     2101 aaaatgggga agactattcc ggttcctgaa gagctgatgg atgctgcgac tgtaataggg
     2161 gcatgcggca ttgcgttcgc attaagattc atgagggcca tgtcacaggg agggattgag
     2221 ataggcttca atgcagaaat gtcgcagatg attacagcac agacggtacg gggagcagcc
     2281 cggcttatca tggaaagcgg caatcatccg gaaaaagaaa ttgacaaggt aaccacaccc
     2341 cagggcataa ctatttccgg actgaacgag atggaacacc agggcctcag ttcggcagta
     2401 ataaaaggct taacagtatc tttcagaaag ctggagaacc tgtcttcctc cgggaaataa
     2461 gcggattgtg cccggaaaca aatcatcctt ttcccgggta tgaagcaccc ttttacaaat
     2521 atcagttgct ctgactgaag
//
LOCUS       DAOSPX010000408         2539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_37193_length_2539_cov_4.128824, whole genome shotgun sequence.
ACCESSION   DAOSPX010000408 DAOSPX010000000
VERSION     DAOSPX010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37193_length_2539_cov_4.128824"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLZ75_13790"
     CDS             <1..381
                     /locus_tag="PLZ75_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS73512.1"
                     /translation="GIELCLEGFRFADILRWKRGELMEMEWNGFYVPELNIPMDLNED
                     GIPDVAFYQGTKPSPEVSGVTYVDVSPTIGGKTNPQLLKNGTSGELTWMNNIKRKWED
                     KMYYYPIPATDLITNPNLGQNPGW"
     gene            533..1309
                     /locus_tag="PLZ75_13795"
     CDS             533..1309
                     /locus_tag="PLZ75_13795"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOS73513.1"
                     /translation="MRNLLLILLSAITVLIVSAGGCDANKQKSTLTVKILSYNVRNCR
                     GMDEVVDYQRVADVIRRIDAEVVALQELDSATQRSGGVVVLDELARLTGMYQTFGASI
                     DYQGGKYGIGILTKEKPVSWKRIPLPGREEARSLLIVELKDLVLGCTHFSLNQEDRLA
                     SAGIVSSSFSGYDKPVFLAGDLNAVPESEVMKRFESYWNILTNTSVPTIPSDKPSKCI
                     DYILGLKSDGRTFVTNKTVVEDEPVASDHLPVWAEIEVAY"
     gene            1684..>2539
                     /locus_tag="PLZ75_13800"
     CDS             1684..>2539
                     /locus_tag="PLZ75_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS73514.1"
                     /translation="MKKLFLMLLLFFAFTVVHAQRITVTGTVSDQQGAPLPGATVQVK
                     GTNFGTITDSNGNYSLEVPGSNSILVFSFVGYVQREAQVLNQTVINTTLREELLGLEE
                     VVVVGYSTQKKLNLTAAVDQVTNEALENRSVPNLTQGLQGVLPNLNINLLDGRPNQAP
                     SYNIRGITSIGQGGSALILIDGVEGDPSMLNPNDIASVSVLKDAASASIYGARGAFGV
                     VMITTRNPEKGKTMVNYTSNLSTKKPIHLPEFVTDGYTWVSMFVAAFLNGDGSFPQNI
                     KKTQKVSQA"
BASE COUNT          745 a          472 c          616 g          706 t
ORIGIN      
        1 ggaattgaac tctgtcttga aggattcagg tttgccgata tcctcaggtg gaagaggggc
       61 gagctcatgg aaatggaatg gaacggattc tatgtccccg aactcaatat tcccatggat
      121 ctcaatgagg atggcattcc ggatgttgca ttctaccagg gtacgaaacc ctctccggaa
      181 gtatcaggag tgacatatgt tgatgtgtct cccaccattg gcgggaaaac caatccgcag
      241 ctgctgaaaa acggcacaag cggtgaactc acctggatga acaatattaa aaggaaatgg
      301 gaggataaga tgtattatta tcccattccg gccacagatc tgatcaccaa tccgaatctg
      361 ggacaaaatc cgggatggta aaaggctgat atgcagagaa ataatttatt ctgaccgcgg
      421 ggatcgcaga gagtatgcgg aaagcgcagg gcaaaaggga ggagagcccc gctgtctctg
      481 cgatccggcg gttcagatta tttatcaata tttaaaccaa aatttacaga aaatgagaaa
      541 tctgctgttg atattactgt ctgccattac ggtgcttatt gtttctgcag gtggttgtga
      601 cgcgaacaaa cagaaatcaa cacttacagt taaaatcctg agttataatg taaggaattg
      661 ccggggcatg gatgaggttg ttgattatca aagagtggct gatgtaatca ggcgtattga
      721 cgctgaagtg gttgcattgc aggaactgga cagtgcaaca caaagaagcg gaggtgttgt
      781 cgtgctggat gaacttgcaa ggctgacagg aatgtatcag acattcggag catcgattga
      841 ttaccaggga gggaaatacg gaataggcat tcttacaaag gaaaaaccgg tgagctggaa
      901 gcgcatacct cttccgggca gggaggaagc caggagcctt ctgatagtcg aactgaagga
      961 tctggttctg ggctgtaccc atttctccct gaaccaggaa gacaggctgg catcagcggg
     1021 catagtgagc agttcattta gcggttatga caaacctgtc tttcttgcag gcgacctgaa
     1081 tgctgtacct gaatcggagg ttatgaagag gtttgaaagt tactggaata ttcttacaaa
     1141 tacttcagtg cctactattc cctcggataa gccttcaaaa tgcattgact acattctggg
     1201 cctgaaaagt gatggcagaa cctttgtaac caataaaaca gtggttgagg atgagcctgt
     1261 tgcttcagat catctgcccg tgtgggctga aatagaggta gcctattagt gttattgaag
     1321 gcatttgttt cgggaatagc ttaattcatc cggataataa gttaagttca aataaatagt
     1381 tgttgagttt tcacacacaa atgatgatct gattgttata atgcagaagt atacaatatg
     1441 cgtgcaggga atttataaac ctgccggttt cctgtaaact gctggaatga agcatgagcc
     1501 gtaacggaga ttacagggac aatgcagtgg ctgaaagctt ctttaaaaca ctcaggatct
     1561 aatatttata tcatatagta aacaaaccat aattagtttt tgctttttgt ttattgtttt
     1621 tgtaaatttc ttctcgatag acttttaatg ataaccttta ttttcaacta atcagatttt
     1681 tttatgaaaa aactattcct gatgttgttg ttattttttg catttacagt tgttcatgca
     1741 cagcggataa cagttaccgg aacagtgtcg gatcagcagg gtgctcccct gccaggtgct
     1801 actgttcagg taaaaggcac taattttggg actattaccg attcaaatgg taattattca
     1861 cttgaggttc ccggttcaaa ttcgattctg gttttctcgt ttgtcggtta tgttcaaaga
     1921 gaagctcagg tcctgaacca gacggtaata aatacaactt tgcgggaaga gcttctcggc
     1981 cttgaagaag tggttgttgt cggatacagt acacagaaaa aactgaatct gacagctgct
     2041 gtagatcagg ttacaaatga agctttagaa aacaggtccg tacctaatct gactcagggt
     2101 cttcagggag ttctgcccaa ccttaatata aatcttcttg atggcaggcc caatcaggct
     2161 ccaagttata atatcagagg tataacctcg attggtcagg gaggaagcgc gctgatactt
     2221 atcgatggtg ttgagggaga tcccagcatg ttgaatccca atgatatagc cagtgtgtca
     2281 gtattgaaag atgctgcttc tgcatctatt tacggtgcaa gaggagcatt tggcgttgta
     2341 atgattacca ccaggaatcc tgaaaaaggc aaaacaatgg taaattacac ctcaaattta
     2401 tcaacgaaga aaccgataca tcttccagaa tttgttactg acgggtacac ctgggtttca
     2461 atgtttgttg ccgcattttt aaatggggat ggatctttcc ctcagaatat aaagaagacg
     2521 cagaaagtat cacaggctt
//
LOCUS       DAOSPX010000409         2536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_37246_length_2536_cov_4.541314, whole genome shotgun sequence.
ACCESSION   DAOSPX010000409 DAOSPX010000000
VERSION     DAOSPX010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37246_length_2536_cov_4.541314"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..581)
                     /locus_tag="PLZ75_13805"
     CDS             complement(120..581)
                     /locus_tag="PLZ75_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73515.1"
                     /translation="MKKLLFFLFLIPLAAPAQDSKAEDIFNSSNCLISVMETFVNDSL
                     VSAYITFDAKDDRLATLKNYFTLCYDTPLNVYKFISELEAFAADADAATAQIGKHKVE
                     IEKPAGTKTVKVYDEREMIFHRFTPKVIAAIRSSLEAWALKHNLRLELQEK"
     gene            complement(806..>2536)
                     /locus_tag="PLZ75_13810"
     CDS             complement(806..>2536)
                     /locus_tag="PLZ75_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOS73516.1"
                     /translation="ITKDGYFSSGPYLKGNVSSGFASANRLLSGHADVSTGSAKWYFR
                     LSGTYSDAGDIRTPEGIMPNSRYDYNNVTAKLGLKPFNDHIFKLQLQRYQANDVGIPG
                     GDAFPGPATASYTGIGRDLISADYEIRNLGNVLSSLKFGYFNQYIDRFVSLKPNTVTE
                     TTLPNGNIQRTKPELFTPTGKHLTNGGQIQSTWNLSPGNTLIAGADVWSRKLTTERIK
                     YITVEVINPSGDIIKTNNVVRGETPTPESSFSSAGLFIQDEARLLDDRLTLIMGGRLD
                     GVLIKNKENYDVDYITINDVQNNFPDRRLTFVEGSERNISWSANAGLLYNLTGNTDLS
                     LNLARSFRSPSLEERFKYIDLGNYVRLGDVSLAPESGFSVDLGLRKWNRDLNFQAGVF
                     VNHMTNMIVEVPGKFIYKAFTGISEYTIDTLPALINSNVSRALLYGFDYEFEYNFYNN
                     FVLFSTGSYVRGKDTEAGENLPLIPPLRGRLGLRYTRHGIASAELIVTGVSRQAKIAQ
                     GEKETEGYIRLDMIVSSDNIKIGKTGLQLFAGIDNILDKSYTNHLSTNRGSISVEPGR
                     NFYLRLNFSF"
BASE COUNT          667 a          578 c          567 g          724 t
ORIGIN      
        1 taaagataca aaaatttctg aaatcaaaat ttcttcatat tgaaagagca ttccggccaa
       61 agcaccggac gaggggaata aacaccggaa tgcagaatgg atgggctgga gaagtgaact
      121 tacttttcct gcaattcaag tctgagattg tgtttcagtg cccatgcttc cagacttgat
      181 ctgatggctg caattacttt cggggtgaac ctgtgaaaga tcatttcgcg ttcatcatag
      241 accttcaccg tttttgtccc ggccggtttt tcaatttcaa ccttatgctt gcctatctgc
      301 gccgtggctg cgtcagcatc agcagcaaaa gcttccagtt cactgataaa cttataaaca
      361 ttcagaggag tgtcataaca caatgtgaaa taattcttaa gcgtagccag cctgtcatcc
      421 ttcgcatcaa aagtgatgta ggcggaaaca agtgaatcgt taacaaatgt ttccatcaca
      481 ctgataaggc agttggagct gttgaaaata tcttcagcct ttgagtcttg tgccggcgcc
      541 gccaggggta ttaaaaacag gaagaataat aactttttca tgtgcagata attttagttt
      601 taaatgacct gacaatacag ccatgaccgg caaatactca ttccttgcag tgctttgtca
      661 atcatggctt attcataata cctgaacagg attatgtcaa aaccagtttg actttatcaa
      721 agttatgaaa gacttcaaaa caagtcaagt aaaaccaccg ggaaagagag tgtttcccgg
      781 aaggaccgca tcaacagtct tccgatcaga atgaaaagtt cagtctcaga tagaaattgc
      841 ggccgggttc aacactgatg ctgcccctgt ttgttgaaag atggtttgta tagcttttat
      901 ccaaaatatt gtcaatgccg gcaaagagct gcaggcccgt ttttccgatt tttatattgt
      961 ctgagctgac aatcatgtcc aggcggatat atccctccgt ttccttttca ccctgtgcta
     1021 tttttgcctg tctggatact cccgtgacaa taagttccgc cgaagctatc ccgtgacggg
     1081 tataacggag tccaagccgg cctctcagag gcggaataag cggaagattc tcaccggctt
     1141 ctgtgtcctt tccccgaaca taggatccgg tgctgaagag aacaaaatta ttgtagaaat
     1201 tatattcgaa ttcatagtca aatccgtaca acaatgcccg gctgacattg gaattgatca
     1261 gcgccggcag ggtatcaatg gtatattcgg agattcctgt aaaggctttg taaataaatt
     1321 tcccgggcac ttcaactatc atattggtca tgtggttgac aaaaacacct gcctggaaat
     1381 ttaaatccct gttccatttt cttaatccca ggtcgaccga gaagccgctt tcgggagcaa
     1441 gtgacacgtc acccagccgg acataatttc ccagatcaat atatttgaaa cgttcctcaa
     1501 gggaaggtga cctgaatgaa cgggccaggt taagggaaag gtcagtattg ccggtcaggt
     1561 tgtacagcag ccctgcattg gcactccacg atatgttcct ttcagatcct tcaacaaatg
     1621 taagcctgcg gtctggaaaa ttgttctgca catcatttat tgtaatgtag tctacatcat
     1681 aattttcctt gttttttatc agcacaccgt caagcctgcc tcccattatc agtgtcagcc
     1741 tgtcgtccag caggcgtgcc tcatcctgta tgaaaagacc tgcactgctg aaggaagatt
     1801 ccggtgtcgg ggtttcaccc ctgaccacat tgttggtttt gattatgtcg cctgaaggat
     1861 tgataacctc cacagtgatg tattttattc tttctgtggt cagtttacgg ctccatacat
     1921 ccgctccggc aataagtgta ttgcccggcg acagattcca tgtgctctgt atctggcctc
     1981 cattggtcag atgctttcct gtgggggtaa acagttcagg ctttgtgcgc tgaatatttc
     2041 cgtttggaag tgttgtctcc gttaccgtat tgggtttcag tgaaacaaag cggtcaatat
     2101 actggttgaa atatccgaat ttaagagagc tcagcacatt gcccaggttc ctgatttcat
     2161 aatcggccga tataagatct cttcctatgc ccgtataaga ggcggttgcc ggtccgggaa
     2221 aggcatcacc tcccggaatg cccacatcat ttgcctgata acgctgaagc tgaagcttga
     2281 aaatatgatc attgaagggt tttagtccga gtttggccgt tacattgttg tagtcgtagc
     2341 ggctgttcgg cattattccc tcgggagtac ggatatcacc ggcatcactg taggtgccgc
     2401 tcagtctgaa ataccatttt gccgagccgg tgctgacatc cgcatgcccc gataaaagcc
     2461 tgttggctga agcaaagcct gagctgacat ttcctttcag atagggtccg gaagaaaaat
     2521 atccgtcctt tgttat
//
LOCUS       DAOSPX010000410         2534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_37278_length_2534_cov_4.388463, whole genome shotgun sequence.
ACCESSION   DAOSPX010000410 DAOSPX010000000
VERSION     DAOSPX010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37278_length_2534_cov_4.388463"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLZ75_13815"
     CDS             complement(<1..1084)
                     /locus_tag="PLZ75_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS73517.1"
                     /translation="MKKPVLIFSVILFLVNIYFLKGQQFVANYDESKVPSYRLPDPLK
                     FENGGTVRNKKGWDRRREEIFKIFENEVYGVAPGWKGEIIASELSSDENALNGKAVRK
                     EINLTLKNGNRELTMTMLLYMPKNSAGSPVFLGYNFYGNHTITSEPGIAVTKSWIRNN
                     KDYGVFENRASEAGRGLSSGYWPVEMIVSEGYGVATIYYGDADPDYDDGFHNGVHGLY
                     DQERDGTSWGTIAGWAWGLSRAMDYLVTLPSVDPGRVIVMGHSRLGKTALWAGAMDER
                     FALVISNNSGCGGAALSKRAFGETVGSINRSFPHWFCGNFRKYNEKEELLPVDQHQLI
                     ALIAPRPVYVASAEEDQWADPRGEFLS"
     gene            complement(1198..2316)
                     /locus_tag="PLZ75_13820"
     CDS             complement(1198..2316)
                     /locus_tag="PLZ75_13820"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOS73518.1"
                     /translation="METKSMSSPSRLPGRVTSVDFFRGFTMFLLAGEATHLYGKLLKI
                     DSGFFHFLGTQLDHHEWHGLHFWDLIQPFFMFIVGVAIPFAVANRLRKGHTIRQINRH
                     AFKRAFLLLFFGWALYFIEADHFVLQFQNVLAQLSVTYLVAFLIRDKSFRFQLIFTLA
                     ILLFIDLAYRFFPVQGFNNPWVPYENLGAWFNNKIEGVEKASIWATLNFVSTTAHTVW
                     GVLCGRLLMSDKTQKEKIKLLIIAGVSALAVGYALDLLSITPIIKKIATSSFVFASGG
                     WTILALCLCYWLIDVKKLFPKFAWFFIVVGMNSIFIYIFFHIGSGDLIQKMIGPFMKL
                     FFSWTGPLMVSILTSLGVWAGFWYVTYWLYKHKIFIKI"
BASE COUNT          746 a          599 c          517 g          672 t
ORIGIN      
        1 atgaaaggaa ttcccccctg ggatcagccc actggtcctc ttcggcactg gcaacataaa
       61 ccggtctggg ggcgataagg gcaatcagct gatgctgatc aacaggcagc agttcctctt
      121 tttcattata tttcctgaaa ttaccgcaaa accagtgggg gaatgatctg tttatgcttc
      181 cgactgtttc gccgaatgcc ctttttgaaa gagcagcacc gccgcatccc gaattattgg
      241 aaatgaccaa ggcgaatctt tcatccatgg cacctgccca aagtgcggtt tttccgaggc
      301 gggaatgccc catgactatc acccttccgg gatcgactga cggaagtgtg acaagataat
      361 ccatcgcacg ggacaggccc catgcccacc cggcaatggt tccccatgat gttccatccc
      421 tttcctgatc atataatccg tgtactccgt tatggaaacc gtcatcataa tcaggatcgg
      481 catctccgta atatatagtg gccacaccgt agccttccga aacaatcatt tcaacaggcc
      541 agtacccgga agacaggccc ctgccggctt cggatgccct gttttcaaat acaccataat
      601 ccttattgtt ccttatccag cttttcgtaa cggcaatacc gggttcagaa gtaatcgtgt
      661 gattaccgta aaaattatat ccaaggaaaa caggtgatcc tgcagagttt ttcggcatgt
      721 acaggagcat tgtcattgtc agctccctgt tgccattttt cagagtcaga ttaatttcct
      781 tccggacagc ctttccgttc agtgcgttct catcagatga cagttcactg gctatgatct
      841 cacctttcca ccctggtgca accccatata cttcgttttc aaaaatttta aatatctctt
      901 cccggcgcct gtcccagcct ttcttattgc gcactgttcc gccattttca aatttcagcg
      961 gatccggaag cctgtatgac ggaactttcg attcatcata attggcaaca aactgctgtc
     1021 ccttcagaaa ataaatattt acaaggaaca gaataacaga aaatatcaga accggtttct
     1081 tcatgtttat taagtatttt agttatacct gaatagccct tcttgctccg gtcgcatggt
     1141 accggcacaa gaggattgta taaacggtac ggctgcccct gaaatattat gtcagagcta
     1201 tatttttatg aatatcttgt gcttgtaaag ccagtaggtc acataccaga atccggccca
     1261 gacgccgaga ctggtcagaa tgcttaccat gagggggcct gtccatgaaa agaataattt
     1321 catgaaggga ccaatcattt tctgtatcag gtctccgctc ccgatgtgga aaaatatata
     1381 tataaaaata ctgttcattc ccacaacaat gaaaaaccat gcaaatttcg ggaacagttt
     1441 cttaacatca atcagccagt agcacaggca aagtgccagt attgtccatc ccccgctggc
     1501 aaagacaaag gaggaagtag caattttctt gataatgggt gtgatactaa gcagatcgag
     1561 ggcatagccc actgccagtg cagaaactcc ggcgataatc agcaatttta ttttctcctt
     1621 ttgtgtttta tcactcatca ggagcctgcc gcaaagtaca ccccatactg tatgggctgt
     1681 tgttgaaaca aagttaaggg ttgcccagat actggccttt tcaacacctt caatcttgtt
     1741 attgaaccat gcacccagat tctcgtaagg cacccaggga ttattgaatc cctgaacagg
     1801 gaaaaagcgg tatgccagat cgatgaacag caaaatagca agtgtgaata tcaactgaaa
     1861 cctgaaactt ttatctctta tcagaaaagc aacaagatag gtaacagaca attgtgccag
     1921 gacattctga aactgcaaaa caaaatgatc ggcctcgatg aaatacaggg cccatccgaa
     1981 aaacagcagt aaaaatgctc tcttgaaggc atggcggttg atctgccgga tagtatgtcc
     2041 ctttcttaac cggttggcta cagcaaaagg aattgccact ccgactataa acatgaaaaa
     2101 aggctgtata agatcccaga aatgaagacc atgccattca tgatgatcaa gctgggtgcc
     2161 aaggaaatga aaaaacccgc tgtcaatctt cagcagttta ccataaaggt gtgtagcttc
     2221 accggccaga agaaacattg taaaaccacg gaaaaaatcg accgaggtca ctcttccggg
     2281 aagtcgggat ggggatgaca ttgatttggt ttccatagag tccggaacaa tttaagtttg
     2341 attcgataaa atctttagta tcaacgttgc tgtaaaagtt taataatatt attcaaactt
     2401 aagaatattt taaaatttaa tggagttctg atagaaaata ttaaacaaca ttataccatt
     2461 ccctccggtc aaagttcatc cgggtatatc cgtcaggcat aaggccggaa cagtttaatc
     2521 agcctgcata atcc
//
LOCUS       DAOSPX010000411         2528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_37406_length_2528_cov_3.745653, whole genome shotgun sequence.
ACCESSION   DAOSPX010000411 DAOSPX010000000
VERSION     DAOSPX010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37406_length_2528_cov_3.745653"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="PLZ75_13825"
     CDS             <1..111
                     /locus_tag="PLZ75_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOS73519.1"
                     /translation="AAGDVQDHVYRQAVTAAGSGCMAALDAERYLQERKK"
     gene            complement(157..795)
                     /locus_tag="PLZ75_13830"
     CDS             complement(157..795)
                     /locus_tag="PLZ75_13830"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HOS73520.1"
                     /translation="MKRDKENINLLKAYNMLLYFAGSMIMKEPSDECINDFWTRGTVK
                     NLPVKSSNPRFIKAASILRDSCRDKDTCREKMVADYRRLFDSDGPRLVPVCESEYTDR
                     LNDDFPARTTVDEFYRTYGWNPDQHHHVPHDHLGIELLFLTRLTDKFTSFDDEPCMRE
                     LGKEIIRFINLHILSWVPAWNDRMQQHAQSECYKGVGYLIYACIEDIRGILS"
     gene            complement(943..2187)
                     /locus_tag="PLZ75_13835"
     CDS             complement(943..2187)
                     /locus_tag="PLZ75_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS73521.1"
                     /translation="MDIFKKITSTKGALGQYSEVGDGYFMFPKLEGEIAARMKFRGKE
                     VLTWSLNNYLGLANNPEVRKADAEYTAQYGLGYPMGARMMSGQTSMHEKFEAMAAEFV
                     GKESAYLLNYGYQGMVSIIDALVDRHDVIVYDSESHACIMDGLRLHFGKRFVYSHNDM
                     ESCEKQLQRATKLTEGTNGGILLITEGVFGMAGDLGKLDEIVKLKEKYNFRLLVDDAH
                     GFGTMGESGAGTGEHFGVQDQIDLYFATFAKSMAGIGAFIAGDKDIITYLRFNLRSQI
                     YAKSLPMAMTLGAIKRLEIIKTQPEHKEKLWVIVKALQKGLREAGLDLGKTQSPVTPV
                     FMKVTLFQTTQLIYDLRENFGIFCSVVIYPVVPRDVVMLRLIPTSTHTLEDVEYTVNA
                     FRELKWKVDNDIYPHEVADFSK"
BASE COUNT          686 a          587 c          517 g          738 t
ORIGIN      
        1 gcagccggtg atgttcagga tcatgtttat cgccaggctg ttacggcagc aggatccggc
       61 tgtatggcag cacttgatgc cgaacggtat ctccaggaac gtaaaaaata aacggttaat
      121 ctcccgggcc ggaataactg accggcggac ggaatatcag gagaggatac cgcggatatc
      181 ctctatgcag gcataaataa gatatcctac gcccttatag cattcactct gtgcatgttg
      241 ctgcatccgg tcattccatg ccggaaccca tgacaggatg tgaagattaa taaatcttat
      301 gatctccttc ccgagctcac gcatgcatgg ttcatcatca aagcttgtga acttgtcggt
      361 gagccgggtc agaaagagca gttcaatgcc aagatgatca tgcggtacat gatggtgctg
      421 atccggattc cagccatatg tcctgtaaaa ctcatccact gttgtcctgg cgggaaaatc
      481 atcattcagc ctgtcagtgt attctgattc acaaaccggt acaagccggg gaccgtcact
      541 gtcaaagagc ctccggtagt cagcaaccat tttctcccgg caggtatcct tatccctgca
      601 tgaatccctt aatattgaag ctgcctttat gaaacggggg tttgaacttt ttacaggaag
      661 atttttcaca gtgcccctgg tccagaagtc atttatacat tcatcactgg gctctttcat
      721 gatcatgctg ccggcgaaat aaagcagcat attataggct ttaagaaggt ttatattttc
      781 tttgtccctt ttcattatct gcattctctg atgtgaaagt ggcttatcag acaaattcac
      841 tgaactcaaa tctgtataac caaaaaaaaa ctgatttgtt cggacaggaa taaaaaagag
      901 gctgtccttt gagacagcct cttcagcctg tatttaattc agttattttg aaaaatcggc
      961 tacttcatgc ggataaatat cattatcgac cttccacttg agttccctga atgcattcac
     1021 ggtatattcc acatcttcga gtgtatgtgt tgatgtgggg atgagtctga gcattaccac
     1081 atccctggga acgacaggat atattacaac cgagcagaaa atgccgaaat tctccctgag
     1141 gtcataaatc agctgggttg tctgaaagag agtaactttc ataaaaaccg gagttaccgg
     1201 cgattgtgtt tttcccaggt ccagacctgc ttccctcaat cctttctgaa gagcctttac
     1261 aattacccac agtttttcct tgtgttccgg ctgggttttg atgatttcaa gacgttttat
     1321 tgcgcccagt gtcattgcca ttggaagaga cttggcatat atctgtgatc tcaggttgaa
     1381 gcgaagatag gttatgatat ccttatcacc tgctataaaa gcgcctatgc cggccatcga
     1441 cttggcaaaa gtggcaaagt agagatctat ctgatcctgg acaccgaaat gttcacccgt
     1501 acctgcaccg ctttcaccca ttgtcccgaa gccgtgagca tcatcaacca gaagcctgaa
     1561 attgtatttc tccttcagct ttactatttc atcaagtttg ccaagatcac ctgccatgcc
     1621 aaacactcct tcggtaatta aaagaatccc tccgtttgtt ccctcggtca gttttgtggc
     1681 tctctgaagc tgtttctcac agctttccat atcattatgg gaatatacaa acctctttcc
     1741 gaaatggagc ctgaggccat ccataatgca tgcatgcgac tcggaatcat atacaatcac
     1801 atcatgacga tcaacaagcg catcaattat tgaaaccatt ccctgataac catagttgag
     1861 aagatatgct gattccttgc caacgaattc agccgccatt gcttcaaatt tctcatgcat
     1921 tgaagtctgg cccgacatca ttcttgcccc catgggatat cccagtccgt attgggcggt
     1981 gtattcggca tcggctttcc gtacttccgg attgttggca aggccaagat agttgttaag
     2041 gctccatgta aggacttcct ttccccggaa tttcattctg gcggctattt ccccttcaag
     2101 tttcgggaac atgaaatagc cgtccccaac ctctgagtat tgtccgagag ccccttttgt
     2161 agaggtgatc tttttaaaaa tatccatagt aacaggtttt aataaaattt taacaaaagt
     2221 agggatatta gcggttattc cataatacct ataaatacct aattttatac attaattctg
     2281 ccgctaaata tttcaggccg gataatacaa ataacccgga ttcgttatga tcagggaccc
     2341 ggacaggcag taaacacctg aacgaggtta aaattcacat ttatatgacc cgaatgacaa
     2401 aaaaatatta taattttgcg caacattttt ctatgagatc gcttgcatat atatttctgt
     2461 tgttttcagt cctggcactg tcatgcggtg agtatgaaaa actgcttaag agcagtgatt
     2521 atgacctc
//
LOCUS       DAOSPX010000412         2512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_37734_length_2512_cov_3.830281, whole genome shotgun sequence.
ACCESSION   DAOSPX010000412 DAOSPX010000000
VERSION     DAOSPX010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37734_length_2512_cov_3.830281"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLZ75_13840"
     CDS             complement(<1..143)
                     /locus_tag="PLZ75_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS73522.1"
                     /translation="MTIRNKSWYPWLVVALLWMVALLNYLDRQMLSTMKLAIMGDIRE
                     LEMA"
     gene            complement(171..1355)
                     /locus_tag="PLZ75_13845"
     CDS             complement(171..1355)
                     /locus_tag="PLZ75_13845"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOS73523.1"
                     /translation="MLNDFSTLAEQYKKELLNSVIPFWEKFSPDKEHGGYFTCLDRTG
                     EVYDTDKFIWLQGRQIWMFSTLYNNVEKNEAWLQTAVHGADFLEKHGRDKNGDWYFSL
                     NREGRPLIVPYNIFSDCFATMAFGQLAKATGNDHYSRIAVNTFRNILSRENNPKGVYN
                     KTIGGTRPLKGFSLPMILCNLSLEIEHLLDRSFVDETIDRCIHEVMEVFYDRETGLIR
                     ENVNTDGSFSDSFEGRLINPGHGIEAMWFIMDLAVRNNSRDLLERAVRITLDILNYSW
                     DNEYGGIFYFMDIKGKPVQQLEWDQKLWWVHMETLISLIKAYYHTREPECLRWFKTLH
                     EYTWTHFPDPAYGEWYGYLNRRGEVLLPLKGGKWKGCFHVPRGLYQVWRILEKLSAEQ
                     AG"
     gene            complement(1378..2304)
                     /locus_tag="PLZ75_13850"
     CDS             complement(1378..2304)
                     /locus_tag="PLZ75_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOS73524.1"
                     /translation="MLKFKKFEGLVAAPFTPMDSKGNLVPELIPEYYDFLAVNGVTGA
                     FINGSTGEGPSLTQKEKFLQAEQWAKCLKNDGRVRVINMVGGTSYGECRENAVFSYEI
                     GLSAVAMVAPYYFRPANPGALAEFVAMTGESVPEMPVYFYHIPVLTGVKIPMLEFLKK
                     ITNMLPNFAGLKYTDEDLMDFMSCINYEGGTYDMLYGRDECMLPALALGCRGFVGSTY
                     NYAAPLYHALISAFDKGDLKKARELQQKSIDMIYLLDKYGGMNTGKAYMKLAGFDFGK
                     FRTPVTRMADERFNEFREDVRLLDMDHLFSKK"
BASE COUNT          666 a          655 c          477 g          714 t
ORIGIN      
        1 gccatctcca gttccctgat atctcccatt atagcaagct tcatcgttga aagcatctgc
       61 ctgtcaagat aattaagcag ggctaccatc cacaggagcg ctacaaccag ccaggggtac
      121 catgatttat ttcttattgt cataaggcta ttgctgggaa aatactatgt tcatccggcc
      181 tgttctgcgg aaagtttctc aagtatccgc cacacctgat acaagcctct cggtacatgg
      241 aagcagccct tccattttcc gcccttcagt ggaagaagaa cctcccccct cctgttcaga
      301 tatccgtacc attccccata cgcgggatcc gggaaatgtg tccaggtata ttcatgcagg
      361 gttttaaacc acctcaggca ttccggctca cgtgtgtgat aataggcctt gatcagactg
      421 ataagtgttt ccatatgaac ccaccagagt ttctggtccc actccagctg ctgcacaggc
      481 tttcccttta tatccataaa atagaatatc cccccgtact cattatccca gctgtaattc
      541 agtatatcga gagttatcct gactgctctt tctagtaaat ccctgctgtt gttcctcacg
      601 gcaagatcca tgataaacca cattgcctca atgccgtggc cgggattaat caaccggcct
      661 tcaaaggaat cagaaaaact tccgtccgta tttacgtttt ccctgatcag accggtttcc
      721 ctgtcgtaaa atacttccat cacttcatga atgcagcggt caattgtctc atccacaaag
      781 cttctgtcaa gaagatgctc tatctcaaga gatagattac agagaatcat cggcagggaa
      841 aaacctttca gaggccgggt tccgccgatg gttttattgt aaacaccttt tggattattt
      901 tccctggaaa ggatattcct gaatgtattg actgcaatcc gggaatagtg atcgttaccc
      961 gtagcttttg ccagctgccc gaatgccatg gtggcaaaac aatcggaaaa aatattatat
     1021 ggcacaatca gtggtctgcc ttccctgttc agcgaaaaat accagtcgcc attcttgtca
     1081 cgtccgtgtt tttcaagaaa atccgctccg tgtaccgcag tctgaagcca ggcctcattt
     1141 ttttccacat tattgtaaag ggtggaaaac atccatatct gccgcccctg caaccatata
     1201 aacttatcgg tgtcatatac ctcccccgtc ctgtcaaggc aggtaaaata acctccatgc
     1261 tccttatccg gcgagaattt ttcccagaat ggaataacac tgttcagcag ttctttttta
     1321 tactgctccg caagagttga aaaatcgttc aacatcaata aaactattta tatcctgtta
     1381 ttttttcgaa aagaggtgat ccatgtcaag cagtctgaca tcctccctga attcattgaa
     1441 gcgctcatcc gccattctgg tgacgggtgt cctgaattta ccgaaatcga agcctgccag
     1501 cttcatatat gctttcccgg tattcatacc tccgtatttg tcaagcagat aaatcatatc
     1561 aatcgatttt tgctgcaatt cccttgcttt tttcagatca cccttgtcaa aggcactgat
     1621 aagggcatgg tataaaggcg cagcataatt ataggtgctt cctacaaagc ccctgcatcc
     1681 cagggcaagg gcaggcagca tgcattcatc cctgccgtaa agcatgtcat aggttccgcc
     1741 ttcataattt atacacgaca taaaatccat gagatcttca tcggtatatt tcaatccggc
     1801 aaaattggga agcatatttg ttattttctt caggaattca agcattggaa ttttcacacc
     1861 ggtaagaacc gggatgtgat aaaaatatac gggcatttcc ggtaccgatt cccccgtcat
     1921 tgcaacaaat tccgcaagag cccctgggtt cgccggcctg aagtaataag gggccaccat
     1981 tgcaactgcc gacagtccga tttcatagga aaaaaccgca ttttccctgc attctccgta
     2041 ggaagtaccg cccaccatat tgataaccct cacccttccg tcattcttca gacatttcgc
     2101 ccactgctct gcctgaagaa atttttcttt ctgtgtaagg gacgggcctt cacccgttga
     2161 accgtttata aatgcaccgg taacgccatt tacggctaga aaatcataat actccggtat
     2221 cagttcaggg acaagattcc ccttgctgtc catcggagta aaaggagctg ccacaaggcc
     2281 ttcaaatttt ttgaatttca gcatgataaa tatttatgac aatgtttttt gatttatagc
     2341 tcagaattgt tgaccctccg gatttttccc ggccggttcc gggtgccctt ctaaaatagt
     2401 cccctcaaaa tatcctccac cggaattttc tgaaaataca ggtccctgac gccttcatac
     2461 actatcccta ttgtcttttc atcaaccatg gaaagacagg aatagccgaa tc
//
LOCUS       DAOSPX010000413         2507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_37852_length_2507_cov_2.082790, whole genome shotgun sequence.
ACCESSION   DAOSPX010000413 DAOSPX010000000
VERSION     DAOSPX010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37852_length_2507_cov_2.082790"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="PLZ75_13855"
     CDS             complement(<1..1683)
                     /locus_tag="PLZ75_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOS73525.1"
                     /translation="MIRQNFISIIIICLFLISCSSENRQTFYLNEFHPEDVALLDGPF
                     RQARDLNIKVLLEYDVDRLFATFRKEAGLPEKAELFPNWKGLDGHVAGHYLSAMAMNY
                     TAVKNEECRKRMEYIISELRECQEANGERYPDWGKGYVGGVPNSDTIWSTLKKGDFKA
                     YRSAWVPWYNVHKIFAGLRDAWFYTGNNDAKDIFLKFCDWGIDITSMLSDEQMQSMLD
                     TEHGGMNEIFADAYQMTGNSKYLEAAKRFSHRVLLDSMADGIDNLDNKHANTQVPKAI
                     GFQRIAELTNEKQFIDAGSFFWETVTKNRTLAFGGNSRREHFPDENGYKDFVNEVQGP
                     ESCNSYNMLRLTKGLFRIKQSAQYVDYYERTLFNHILSTQHPEHGGFVYFTPARPRHY
                     RVYSAPNEAMWCCVGSGMENHGKYNQFIYTHSKDSLYLNLFIASELNWKEKGIKIKQE
                     TAFPFEEQTKLTFAGKSAKFTLLVRYPSWVKEGALKVTVNGNSIDYNTRPSSYLANDR
                     TWMDGDVVQIMLPMHNSIEQLPNVPEYVAFLHGPILLAAETGKEDLKGLIADD"
     gene            complement(1724..>2507)
                     /locus_tag="PLZ75_13860"
     CDS             complement(1724..>2507)
                     /locus_tag="PLZ75_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOS73526.1"
                     /translation="MPPFSMLFVHGDGPAFYDVKNVPHGAITTHFYHSPVTNGEREML
                     VYTPPGYDKSKKYPTLYLLGGSGDLAQTWYMHGQANFIMDNLLAEGKAVPMIIVMPNN
                     QMVHRSHPKHTDLTFKLIEREFKEVIIPFVESNYSVIKDRHARAISGLSMGGRHAQYV
                     GLNNLDLFASIGILSAAIGIDQTPALKDPDINSKLDYLFVGAGTHETRPGVRHEVLHN
                     DLVKLNVKHEYYIGSRGAHDLITWRHLLYYRFYPNLFRNLTP"
BASE COUNT          677 a          538 c          495 g          797 t
ORIGIN      
        1 gtcatcagct atcagtcctt tcagatcttc tttacctgtt tcagcagcaa gaagaatagg
       61 tccgtgaagg aaggcaacat attccggaac atttggcaac tgttcaattg aattgtgcat
      121 tggaagcata atctgaacaa catcaccatc catccaggtc ctgtcatttg caagatacga
      181 tgacggacgc gtattataat caattgaatt tccgtttaca gtgactttga gagctccctc
      241 ctttacccac gacggatatc ttaccagaag agtgaatttg gcagacttcc ctgcaaaggt
      301 gagttttgtt tgctcttcaa aagggaatgc tgtttcctgt tttattttaa ttcccttttc
      361 cttccagttc agttccgaag cgataaacag attcaagtat agtgaatcct ttgaatgtgt
      421 ataaatgaat tgattgtatt ttccatggtt ttccattccg ctacccacac aacaccacat
      481 tgcctcattt ggggcagaat agactctgta atgtcgcggc cgggccggag tgaaataaac
      541 aaaaccccca tgttcaggat gctgtgtgga gaggatatgg ttaaataatg tcctttcgta
      601 ataatcgaca tattgagctg attgctttat cctgaatagg ccttttgtca agcggagcat
      661 attatatgaa ttgcatgatt ccgggccctg tacttcattc acaaagtctt tatatccatt
      721 ttcatcaggg aaatgttccc ttctgctgtt gcctccgaat gcaagggtcc ggttttttgt
      781 tactgtttcc cagaaaaaac tgcctgcatc aatgaattgc ttttcatttg tgagttctgc
      841 tatccgctga aaaccaattg ctttaggcac ctgggtattg gcatgtttat tatcgagatt
      901 atcgatgccg tcagccatcg aatcaagcaa tactctgtgg gaaaatcttt ttgcagcctc
      961 gagatatttt gagttgcctg tcatctggta agcatcagca aatatttcat tcattcctcc
     1021 atgttccgta tcaagcattg attgcatctg ttcatcggaa agcatagaag taatgtcaat
     1081 accccagtcg caaaatttca ggaaaatgtc ttttgcatca ttatttccgg tataaaacca
     1141 ggcatctcta agaccggcaa atattttatg tacattatac cagggcaccc atgccgatct
     1201 gtaggctttg aaatcacctt ttttgagagt gctccagatg gtgtcgctgt tgggaactcc
     1261 accgacgtat ccttttcccc agtcagggta tctttcccca tttgcttcct ggcactcacg
     1321 cagttcagat attatatatt ccatgcgttt cctgcattct tcattcttta cggcagtata
     1381 attcattgcc attgcagaaa gataatgccc tgcaacatgg ccatccaatc ctttccagtt
     1441 agggaataat tcggcttttt caggaaggcc tgcttctttc ctgaaagtgg cgaacaatct
     1501 gtcaacatca tattccagga gtacttttat attcagatcg cgagcttgtc tgaacggtcc
     1561 gtcgagcaag gcaacatctt ccgggtggaa ttcattaagg taaaaggttt gccggttttc
     1621 ggatgagcag cttatcagaa acaggcagat aataataatt gatatgaagt tctgtctgat
     1681 cataattgat attattgctt cgtacaaata tttattctta gcatcacggt gttaaatttc
     1741 taaagagatt cggatagaat ctgtaatata ggaggtgtct ccaggttata agatcgtgag
     1801 cacccctgga accgatgtaa tactcgtgtt ttacatttag tttaacaaga tcattgtgca
     1861 gtacctcatg tcttactccc ggtcttgttt catgagtacc ggctccgaca aaaagataat
     1921 caagttttga atttatatcc ggatccttca aagcaggagt ctggtctatc cctatagcgg
     1981 cgcttagaat acctattgat gcaaacaggt caagattgtt taaacccaca tactgtgcat
     2041 gccttccgcc cattgataaa ccacttattg cccgggcatg cctgtctttt attacactgt
     2101 aattactctc tacaaaaggt attataacct ctttaaattc tctttcaata agtttgaatg
     2161 tcagatcagt atgctttgga tgtgaacggt gaaccatctg gttattgggc attactatta
     2221 tcatgggcac tgcttttcct tcagcaagca aattgtccat aataaagttt gcctgtccat
     2281 gcatatacca ggtttgagca agatcccccg atcctcccag aaggtaaagt gtagggtatt
     2341 ttttcgactt atcatatccg ggaggagtat aaacaagcat ttctctttca ccatttgtaa
     2401 caggggaatg gtagaaatga gttgttattg ccccatgagg cacattttta acatcataga
     2461 atgccgggcc gtcaccatgc acaaaaagca tactgaaagg aggcata
//
LOCUS       DAOSPX010000414         2472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_38613_length_2472_cov_3.395532, whole genome shotgun sequence.
ACCESSION   DAOSPX010000414 DAOSPX010000000
VERSION     DAOSPX010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38613_length_2472_cov_3.395532"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..784)
                     /locus_tag="PLZ75_13865"
     CDS             complement(38..784)
                     /locus_tag="PLZ75_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73527.1"
                     /translation="MENKKTTKKITRIEDDGTASAPAGARTTKFVASDESKAKARNFR
                     IIAAFAWIVAIAFEIIAIVQLRNAPVNTTILIVLIVAALIFAVTGSMLWKKANRFDPA
                     SEKDKVRFFVQNQLGAIISIIAFLPLVILIFTNKDLSGKQKGLVGTIAVVALLIAGVA
                     GVDFNPPSQEQYLEQTQQVEALMDGVNHVYWTKSGKVYHLFQDCSYINTDRTDEIFEG
                     TVAKARELKNITNLCSRCEQRAIKEKESRP"
     gene            complement(1013..1519)
                     /locus_tag="PLZ75_13870"
     CDS             complement(1013..1519)
                     /locus_tag="PLZ75_13870"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HOS73528.1"
                     /translation="MKKIYTLMISLIISQILSAQTGSLEALFEKYSEKEGFTSVYISG
                     KMLNMLGAMNRDPDKADNILLRLKSIRILSEKDSLSGGKENFHNELGRILNRSEYEEL
                     MLVREGSDLTRFLVRQKGDIISELLMITKGRQGNSVVSIKGEINLRELADLSRTLGIE
                     NLEHLNTP"
     gene            complement(1528..2160)
                     /locus_tag="PLZ75_13875"
     CDS             complement(1528..2160)
                     /locus_tag="PLZ75_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73529.1"
                     /translation="MEEHRLEVLLEKYYSGRISGKEEQELRDFLCGDAVFPGYEAERE
                     IFLHYSESEKMPVPSPDFETRIIKAIDDLEEAVQQQRPLPGQITEPEQKPLKRYLFTF
                     SGIAAAILLIISLWFFLNLFNHATEPEDTYTDPALAYAEAMKVLSGVSLRLNIAAESV
                     IPVVDLAKASFDNMEPLSKSLNSFSTGLRETGLQMPDTDSDRNKNIPKPK"
     gene            complement(2147..>2472)
                     /locus_tag="PLZ75_13880"
     CDS             complement(2147..>2472)
                     /locus_tag="PLZ75_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS73530.1"
                     /translation="KNSCIDMIRKRKHIDTEWNGNDSADSYLSPSPYEKMVKDESQSI
                     LNRIIDELPPLYRDLIQLKEINGLSYEEISQQSGIKINTLRVTLSRARKIIKAKYLEY
                     VDGRA"
BASE COUNT          633 a          560 c          478 g          801 t
ORIGIN      
        1 gatatgctga tacagtgtgc ctgcctgttg tgttaatcta tggccgggac tctttctctt
       61 taatggctct ctgttcacag cggctgcaca gattggtaat attcttcagt tccctggcct
      121 ttgcaacagt tccctcgaat atctcatctg tcctgtcagt gttgatatag gagcaatctt
      181 gaaagagatg ataaactttg ccggattttg tccagtaaac atggtttaca ccatccatca
      241 gagcttccac ctgctgtgtc tgttccagat actgttcctg tgacggcgga ttgaaatcaa
      301 ctcctgccac acctgctatc agcagagcga ctaccgcaat tgttccgaca aggcctttct
      361 gcttaccgct gaggtccttg ttggtgaaga taaggattac cagtggcagg aaggctataa
      421 tgctgataat agcaccgagc tgattctgaa caaagaatct gactttatcc ttttctgaag
      481 ccggatcgaa tctgtttgcc tttttccaca acattgaacc tgttacggca aaaattaatg
      541 ctgctactat cagaacaatc agaatggttg tatttaccgg agcattccgg agctgaacga
      601 tggctataat ttcaaatgca attgccacaa tccatgcaaa agcagcaata atcctgaaat
      661 tccgggcttt tgccttgctt tcatcgctgg ctacaaattt cgtggtccgt gcgcctgctg
      721 gtgcggaagc cgttccatcg tcctcaatcc ttgttatctt cttcgtagtt tttttatttt
      781 ccattatatc atgttttgtt ggttaaagca attctttccc ggcctgtttt atgcccggag
      841 tataatttcc cttaaatcag ttattgtgtt cttaattgta tattactgga gccggatgat
      901 cgttactaat cagattaatg gctgcaaatt taataattat taacaaaaaa acattcacca
      961 ggttcatttg aggtggaaat agtcctgttt cgttgcagta ttccacatat agctatggcg
     1021 tattcagatg ttcaagattt tcaataccca gcgtccggga aagatcagcc agctccctga
     1081 gatttatttc gcccttgatg gaaaccacag agttaccctg tctgcctttt gtgatcatca
     1141 gtaattctga tatgatatct cctttctgac gtaccaggaa ccttgtcaga tcactacctt
     1201 cacggacaag cataagttct tcatattctg atctgttcaa aatcctgcca agttcattat
     1261 ggaaattttc ctttcccccg gaaagagaat ccttctccga taatatcctt atcgatttaa
     1321 ggcgcaaaag aatattatcg gctttgtcgg ggtccctgtt cattgccccc agcatattca
     1381 gcatctttcc tgaaatataa acggatgtaa agccctcctt ttcagaatat ttttcaaaca
     1441 atgcttcaag ggagcccgtc tgagctgaga gtatctggct gatgatcaga gaaatcatta
     1501 aggtatatat ttttttcatc ttatctttta ttttggtttt ggtatatttt tatttctgtc
     1561 gctatcagta tccggcatct gcagacctgt ttcccttaat cccgtactga aggaattcaa
     1621 ggatttggac aggggctcca tattatcaaa tgatgctttc gcaagatcca caaccggaat
     1681 aactgattct gcagcaatat tcaacctgag tgaaacgccg gaaagaactt tcatagcctc
     1741 agcatatgcc agtgcaggat ccgtataggt atcttcgggc tctgttgcgt ggttaaacag
     1801 gttaaggaag aaccacaaag aaataatcag cagtatggcg gctgcaatcc cggaaaacgt
     1861 aaataagtac ctttttaaag gcttttgttc cggttccgtt atttgccccg gcaatggcct
     1921 ctgctgctgt acagcctctt ctaaatcatc aatagcctta attatcctcg tttcaaaatc
     1981 cggggaagga acaggcatct tttctgattc agaataatgc agaaaaatct ccctttcagc
     2041 ttcatatccg ggaaaaacag catccccgca aagaaaatcc ctgagttcct gctcttcctt
     2101 gccggaaatc ctgccactgt aatacttctc aagcaatact tcaagtctat gctcttccat
     2161 caacatattc aagatatttc gctttaataa ttttccgggc ccgtgataat gtcaccctca
     2221 gtgtattgat ttttattccg ctctgctgtg atatctcttc ataggataat ccgttgattt
     2281 cctttagttg aatcaggtcg cggtataagg gaggcagttc atcaatgatg cggttaagta
     2341 tactctggct ttcatccttt accattttct cataaggtga cggggacaga tatgaatctg
     2401 cggagtcatt cccgttccat tctgtatcaa tatgtttccg ttttctgatc atgtcaatac
     2461 agctgttttt tg
//
LOCUS       DAOSPX010000415         2468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_38707_length_2468_cov_4.279320, whole genome shotgun sequence.
ACCESSION   DAOSPX010000415 DAOSPX010000000
VERSION     DAOSPX010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38707_length_2468_cov_4.279320"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLZ75_13885"
     CDS             <1..316
                     /locus_tag="PLZ75_13885"
                     /inference="COORDINATES: protein motif:HMM:NF025923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOS73531.1"
                     /translation="GEVEGRNIVIIDDMVDTAGTIVLAANMMKERGAKSIRAVATHPI
                     LSGNAVERINNSSLEELVVSDSVPFAVRSDKIKVLSIAALFAGTIRAVNTNQSISTSF
                     VF"
     gene            419..1060
                     /locus_tag="PLZ75_13890"
     CDS             419..1060
                     /locus_tag="PLZ75_13890"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOS73532.1"
                     /translation="MKTIEIKGTLRNELGKKHARQVRKAGNVPCIIYGKEKNINFSAQ
                     ENSIKKLVFTPDAHLVHLNLDGEVTRAILHEIQFHPVTDRILHADFVQVFADKPVTMN
                     IPVAITGDSVGVKAGGRLSVRKRNLKIRALAENLPDNIEINVSSLKINDSIKVGNLKK
                     DKIEFLDSKIATVVMVSSSRVAIKAEGEAEEEVTSGEGTEGTETPAGESADRE"
     gene            1164..1775
                     /gene="pth"
                     /locus_tag="PLZ75_13895"
     CDS             1164..1775
                     /gene="pth"
                     /locus_tag="PLZ75_13895"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOS73533.1"
                     /translation="MKYLVAGLGNVGDEYAGTRHNIGFMVLDAMARASGSLFSDCRYG
                     FISPVKYKGRQLILLKPSTYMNLSGNAVRYWLKREKIELENLLVIVDDIALPLGSIRM
                     RPKGSDGGHNGLAHISSVLGTNEYARIRVGIGNGFRKGSQVHHVLGRWKPGEEEFLKE
                     RIDIVIEMIKSFAVAGTELTMTAYNKMGKMPGKEADGNKTALL"
     gene            1772..2164
                     /locus_tag="PLZ75_13900"
     CDS             1772..2164
                     /locus_tag="PLZ75_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOS73534.1"
                     /translation="MTESKSVRTDKFLWAVRLFKTRTQAANACRMGRVLIDNNKVKPS
                     KYLEGNEIIVLRRPPVTFTYRITGLTENRVGAKLVDNYLEDLTPAGEKIKLEMKRMEM
                     PDGRRKGSGRPTKKERRVIDRWKEFFFL"
     gene            2229..>2468
                     /locus_tag="PLZ75_13905"
     CDS             2229..>2468
                     /locus_tag="PLZ75_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73535.1"
                     /translation="MGRDNPEEYSRGYALLKAIAGFWHNNIFYRKVIVVGRENINPDH
                     HLIFAPNHQNALMDALAVLFTHRGQPVFLARADIFR"
BASE COUNT          768 a          513 c          641 g          546 t
ORIGIN      
        1 cggcgaggtg gaaggcagaa acattgtaat tatcgatgat atggtcgata ccgcgggaac
       61 tatcgtcctt gcagccaata tgatgaagga aagaggtgca aaaagcataa gggcagttgc
      121 aacacatccc atactgtcgg gaaatgccgt ggaacggata aacaattcct cactggagga
      181 actcgttgta agcgattcag taccttttgc cgtcagatcc gacaaaatca aagtgctctc
      241 aatagccgct ttattcgccg gcacaatccg tgcagtaaac acaaatcagt cgatcagtac
      301 aagttttgta ttctgaatgc tttgtaatat atttgcaggc cgatttttga aaaagtccaa
      361 tgtgcgatgg ggagctgcca cgaaggcggc ttttagcagc acaaaaatat attaacagat
      421 gaagacaatt gaaataaagg ggacactgag aaatgaactc gggaaaaaac atgccaggca
      481 ggtaaggaag gccggcaatg tgccatgtat tatttacggg aaagaaaaga atatcaattt
      541 cagcgcccag gagaacagta tcaaaaaact ggtctttacg cctgatgcac atctggtgca
      601 tctgaatctc gacggagagg taaccagggc catccttcat gaaatccagt ttcaccctgt
      661 cactgacagg atcctgcatg ccgactttgt tcaggtcttt gccgacaaac cagtaacaat
      721 gaatatcccc gttgcaataa cgggcgattc ggttggcgtc aaggccggag gcagattaag
      781 tgtaaggaaa aggaatctga aaatacgggc actggcagaa aacctgcccg ataatatcga
      841 gataaatgta agcagcctga aaatcaatga ttcaataaag gttggcaatc tgaaaaagga
      901 caagattgag tttctcgatt caaaaattgc aacggttgtg atggtgtcat catcgcgtgt
      961 cgccatcaag gctgaagggg aagcagaaga agaagttact tcaggcgaag gtactgaagg
     1021 tacggaaact ccggcaggag aatccgccga cagggaatga cgggaagccg ttgatccggc
     1081 ccctccggta caaagaccgg gccgggaaaa gatacgactg aaacggggtg gaaataactt
     1141 aagacttaaa ataatccggc attttgaaat atcttgtggc aggcctgggg aacgtaggtg
     1201 atgagtatgc cggcacaagg cataatatag gattcatggt cctggatgca atggcaaggg
     1261 catccggttc acttttttcc gactgccgct acggatttat aagccccgtg aaatacaagg
     1321 gacgccagct gatcctgctc aagccgtcaa cctacatgaa tctcagcgga aatgcggtaa
     1381 gatactggct caaaagggag aaaattgaac tggaaaacct cctggtaatc gttgatgaca
     1441 tagcacttcc gcttggaagc ataaggatga gacccaaagg cagtgatggc ggacataacg
     1501 gccttgcaca cataagttcc gttctcggaa caaatgaata tgcccgtatc agagtgggta
     1561 taggaaacgg attccggaag gggtcccagg tacaccatgt cctgggccga tggaaacccg
     1621 gggaggaaga gttcctgaag gaaagaattg atatagtcat tgaaatgatt aagtcatttg
     1681 ccgttgcagg aactgaactg acaatgactg catacaacaa gatgggaaag atgcccggaa
     1741 aggaggctga cgggaataaa acagcattat tgtgacagaa agcaaatccg tccggacaga
     1801 caagttcctg tgggccgtcc ggctctttaa gacccgtacc caggcagcaa atgcctgcag
     1861 gatgggaaga gtgcttattg acaataataa ggtcaagcct tcaaaatatc ttgagggaaa
     1921 tgaaattatt gttctccgca gacctccggt tacattcact tacaggataa caggcctcac
     1981 ggaaaaccgt gtgggtgcca agcttgtgga caattacctc gaggatctga cacctgccgg
     2041 tgaaaagatc aagcttgaaa tgaagaggat ggaaatgccg gacggacgga gaaaaggttc
     2101 aggaagacca actaaaaaag agagaagggt cattgacagg tggaaagaat tctttttttt
     2161 ataacttgca actttgcata ttttgatatc agcaggcctg acggcctgaa tcattttttt
     2221 catcgggaat gggaagagac aacccagagg aatattctcg gggatacgcc ttactgaagg
     2281 caattgcggg attctggcat aataatatct tttacaggaa agtaatagtt gtcggccggg
     2341 aaaatatcaa tccggatcat catctgatct ttgcccccaa tcatcagaat gccctgatgg
     2401 atgccctggc tgtcctcttc acacaccggg gccagccggt atttctggcc agggccgata
     2461 tcttcaga
//
LOCUS       DAOSPX010000416         2459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_38872_length_2459_cov_4.050749, whole genome shotgun sequence.
ACCESSION   DAOSPX010000416 DAOSPX010000000
VERSION     DAOSPX010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38872_length_2459_cov_4.050749"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLZ75_13910"
     CDS             complement(<1..626)
                     /locus_tag="PLZ75_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73536.1"
                     /translation="MKKSGIILGIILLAAFCQMLPLNAQEKTKEEKEREMQEAIREQK
                     KIMIERQKAEMERQKAEVERQKADFESRKRAREEFEKAMEDLKEQGKTRPFGDPFMWP
                     GPGDLYRWWPNGNSEKSTWDISKSIKGSSYSREYIFDVEPSAKSVIMSVSGDCKEGEI
                     RIKVIMPNGRTFSEIVIDQYGNLNWRKSLNISDTENQDKTGAWKFSIKS"
     gene            789..2201
                     /locus_tag="PLZ75_13915"
     CDS             789..2201
                     /locus_tag="PLZ75_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS73537.1"
                     /translation="MMKKRNTILLLGITAIAILIIAQIIIIRGILKQNHEMLYLRYRL
                     LSQEALVSLRRQYKVDVYDTVRFILDEYSREVAAELPLIRDEAEMNRKKGEILDYFTM
                     TLNQEEYLSDYLSAYFERMGVEKDFTSRIEIRNLEIISTDTFPIIRTFSFFGKQPPEQ
                     TGGGKTAARTDISPAAGSRLLVERRGLEDNYYSLFFEFFIDFSDKRKMVLQASSASLA
                     MSLFSLVIVVAIFMLTYKNLMEEKRLSDLKTDFINNMTHELKTPLATITVAGKTLEME
                     RIRQDDAKVLETAKLIGKQSVHLNQLINMILEISMWERTEFQLDKKKAVIEEVMNDIV
                     YSFRIGPGNSATITQNYSFNGLEADIDLVYFITMINNLLSNAVKYSARDPVINIEGFA
                     ENGKIFVRIADNGIGISKSDQKHIFEKFYRTSSGNIHKYKGLGLGLYYVKKIAAAHGG
                     DVTVSSKPGKGSIFTVTIPY"
     gene            2218..>2459
                     /locus_tag="PLZ75_13920"
     CDS             2218..>2459
                     /locus_tag="PLZ75_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS73538.1"
                     /translation="MKVLLAEDDRDFGNILAQYITMNGFEVSLARDGREAWEAFTKDR
                     PDICVIDVMMPEMDGFTLGEKIKEAKPEIPFIYLTA"
BASE COUNT          733 a          567 c          511 g          648 t
ORIGIN      
        1 gattttatac tgaacttcca ggcaccggtc ttatcctgat tctcagtatc ggaaatattg
       61 agtgattttc tccagttaag attgccatac tgatcaataa cgatttcgga aaaggtcctg
      121 ccgtttggca taatgacctt tattctgatt tcaccttcct tgcagtcgcc gctgaccgac
      181 atgatgactg attttgcaga aggctccaca tcgaaaatat attcacggga ataagacgat
      241 ccctttatcg atttggagat atcccaggta ctcttttcag aatttccgtt cggccaccat
      301 ctgtacagat cacccgggcc cggccacatg aacggatcac cgaagggcct tgtcttcccc
      361 tgttccttta aatcctccat ggctttctca aattcttccc tggcccgctt ccttgattca
      421 aaatcagctt tctgcctctc cacctccgcc ttctgcctct ccatctccgc cttctgcctc
      481 tcaatcataa tttttttctg ctcccttatg gcttcctgca tctccctttc cttctcctcc
      541 tttgtctttt cctgggcatt cagcggcagc atctgacaaa aagctgccaa aagaattatt
      601 cccagtatta ttcccgattt tttcatggca gtatcttttt taattattgt ctctttaatt
      661 attttcagtt caaagataat accgtttaca gccccgcttt gttaatacgg agttaatgca
      721 gggttaatgt gaggatcagg ggatagtgat cttaagaaaa ttcaatacat ttgataaaca
      781 aactgcagat gatgaaaaaa aggaatacca tactccttct cggcataaca gcgatagcaa
      841 tcctgatcat cgcccagata atcatcatac ggggaatcct gaagcagaat catgaaatgc
      901 tttacctccg gtacaggctg ctttcccagg aagcccttgt atccctgagg cggcagtata
      961 aggtggatgt ttacgatacc gtccgtttta ttctggatga gtattccagg gaagtggcgg
     1021 ctgaactgcc gctgataagg gacgaagcgg aaatgaatag aaaaaaaggt gaaatactgg
     1081 attatttcac aatgacactc aaccaggaag aatacctttc tgactacctc tccgcgtact
     1141 ttgaaagaat gggagtggaa aaggatttta cctcccgaat agaaatccgg aatcttgaaa
     1201 tcatcagcac ggatacattt cccatcatca ggaccttcag cttttttgga aaacaaccgc
     1261 ctgaacagac cggaggagga aaaactgcag cccggacgga tatatctcct gccgcaggat
     1321 ccagactgct cgttgaacgc aggggacttg aagacaatta ttactccctc ttttttgaat
     1381 tcttcattga tttctcggat aagcggaaaa tggtgcttca ggcttcttcc gcatcacttg
     1441 ccatgagcct gttcagcctg gtcatagtgg ttgccatatt catgcttacc tataaaaacc
     1501 tgatggagga aaaaagactg tcagacctca aaacagactt tataaacaac atgacccatg
     1561 agctcaaaac gcccctggct acaataaccg ttgcaggaaa gaccctggaa atggaaagga
     1621 taagacagga tgatgcaaag gttctggaaa cggcaaaact gataggtaaa caaagcgtac
     1681 atctgaacca gctgataaac atgatactcg aaatatcaat gtgggaaaga accgaattcc
     1741 agctcgacaa aaagaaggca gtcattgagg aagtaatgaa cgatatcgtt tacagcttca
     1801 gaatcggacc cggcaactca gccacaatta cccagaatta cagctttaac ggactggaag
     1861 cggatatcga ccttgtctat tttatcacaa tgatcaacaa cctcctgtcc aacgcggtca
     1921 aatattcggc cagggatccg gttataaata ttgaaggctt tgccgaaaac ggaaaaatat
     1981 ttgtcaggat agctgataac ggcataggaa taagcaaatc ggaccagaaa catatttttg
     2041 aaaaatttta ccgtacatca tcaggcaata ttcacaaata taagggactg ggactagggc
     2101 tttactatgt gaaaaaaatt gccgccgccc acgggggtga tgttacggtt agcagcaaac
     2161 ccggaaaagg cagtatcttt acagtaacaa ttccttatta aaccacagag aggcattatg
     2221 aaagttttac tagcggaaga tgacagggat tttggcaata tccttgcaca gtacataacc
     2281 atgaatggct ttgaggtcag cctggcacgc gacggcaggg aagcatggga agcattcaca
     2341 aaagacaggc cggatatatg cgtaatcgat gtgatgatgc cggaaatgga cggatttacc
     2401 ctgggagaaa aaattaagga agccaaaccc gaaatcccct ttatttacct gacagcaaa
//
LOCUS       DAOSPX010000417         2450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_39057_length_2450_cov_3.491858, whole genome shotgun sequence.
ACCESSION   DAOSPX010000417 DAOSPX010000000
VERSION     DAOSPX010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39057_length_2450_cov_3.491858"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            660..2114
                     /locus_tag="PLZ75_13925"
     CDS             660..2114
                     /locus_tag="PLZ75_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73539.1"
                     /translation="MKSTVVRSGILSVLLILSVTGFSQKDPWLSFKLPEPLNPPYSSA
                     VGDSVVLFVDANNSRLIWAFNLKESCWREYHAPSGIAHTASIMAGRGVAMISNDSIII
                     GFSSQKDTLAYLFYEGLPMTGGQYRGAQNDLCWFYTKEKIYVFDGTDGRWHSFPAPAG
                     AFNVLVTAKEKYLAFFTSTTPANFRVLTIYNQLTKNFHQIQFADEYRYIEYLDDGLVF
                     VRRKPGSDDYYGSFTVKNDYIEQKAIPCVLEIANRVSDSDYTDFHYEPRYSVLLTGRT
                     LPSSDFKIYYYIIGFDTRLGYFTRDSVINDISTYEYNVFVGPAYSQNSYAVAAKNYMY
                     KGERYASDLIIYNGLTGSFSTHSVNLTGGKLGIIIHPTINAVLASGIQQWPSNVPTSW
                     QGINPFQGGLTETMMFDLQKHMAYGSSHFYCRDFIMHSYQVGDTLRLLWYNSVFNKIH
                     KYIGIKPNTSSYSLRSGIDYCVISDINRDTRKKM"
     gene            2114..>2450
                     /locus_tag="PLZ75_13930"
     CDS             2114..>2450
                     /locus_tag="PLZ75_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73540.1"
                     /translation="MFYYSALTDSFEESAYTGYSNIMPLDEYVVLLYPDIKKTVIYDP
                     VRGGEVAVNAYPASGNHIQTTRQSVVVYSDHTFYAYSRVKNNQATAQFPENPIFPLLR
                     ESDKVVFIHG"
BASE COUNT          712 a          487 c          546 g          705 t
ORIGIN      
        1 ccttaggtaa cgaagctacg agttggtcat taagacgaag atcgattttt ggagacgatt
       61 ttgccagatg gacaatacac aaatacgatg gggtatccgt tagatgtatt aaagattaaa
      121 gatagtgtcc ctgatgaaaa ctctttttat cctgataaaa tgggaaccat ggaaggatat
      181 gaccttctga atatccagaa ctgaattgga tctgttattg aaactccaaa gtttcaattt
      241 acagctataa gaacacggca tcgggcattt acctgattac tcttcgtcag ataaaggagc
      301 cggaattttc tccggataac ggaaaagatg gttttttatc cgatctccgc gggaagacaa
      361 ttacaaagga agccccggct tatttcctgc acaaattata cctgccggct ctatattgga
      421 aggatggcgg gggaacggac gagataggat ttggtgccag gggcggtggt atcagatacg
      481 gtaacaaccc ttacgtagat ttcgacttac aagagttatg gtgccatttg tggtctatcg
      541 gatggaccgg atacaacgaa gctaatgcta tatgcccaat atttgaacgg tcgtcagatt
      601 gcagaggctg cagcctgaca gtagagatat ctggtaataa attgtaaatc aatttgtcta
      661 tgaaatcaac agtagtaaga tccggtattt tatcagtatt gctgatatta tcggtaacag
      721 gcttttcgca aaaagacccg tggctgagtt ttaaactccc tgagccactt aatcctcctt
      781 attcatctgc ggttggagat tctgtggtat tatttgttga tgctaataac agcaggctga
      841 tctgggcctt taatctgaaa gaatcctgct ggagggagta tcatgcgccg tcaggcattg
      901 cacatacagc cagtattatg gcaggcaggg gtgttgcgat gattagcaat gattccataa
      961 ttattggctt cagttcacaa aaggataccc ttgcgtatct tttttatgaa gggcttccga
     1021 tgaccggcgg gcaataccgg ggtgcacaaa acgacctgtg ctggttttac acaaaagaga
     1081 aaatctatgt ttttgacggg acagatggcc ggtggcatag ctttccggct cctgccggtg
     1141 cttttaacgt gcttgtcact gccaaagaaa agtatctggc attttttaca tctaccaccc
     1201 cagccaattt cagggtatta acgatttata atcagctgac aaaaaacttt caccagatcc
     1261 agttcgctga cgagtatcgt tatattgaat atcttgacga tggactggtt tttgtcagaa
     1321 gaaagccggg atctgacgac tattacggca gtttcacggt aaaaaatgat tacatcgagc
     1381 aaaaagctat cccgtgtgtg ctggaaatag ctaatagggt ctccgactca gattacactg
     1441 attttcatta cgagcccagg tattcagtcc tgctaacggg acgcacactc ccctcttccg
     1501 actttaagat atattactat ataatagggt ttgatacccg tctgggctat ttcacgcgtg
     1561 attcagtcat caatgatata agcacctatg agtacaacgt attcgtgggt ccggcctatt
     1621 cgcaaaacag ttatgctgtt gccgctaaga attatatgta taagggtgaa agatatgcct
     1681 ctgacctcat aatatataac ggacttacag gctctttctc aacacatagt gtaaacctta
     1741 caggtggtaa gttaggaata atcattcatc caacaataaa cgcggtcctg gcttcaggca
     1801 tacaacaatg gccttccaat gttccgacat catggcaggg tattaatcct tttcagggag
     1861 gtctgaccga aaccatgatg ttcgatcttc aaaaacatat ggcttatggt tcaagtcact
     1921 tctattgcag agacttcatt atgcattctt accaggttgg agatacatta aggttattgt
     1981 ggtacaattc agtttttaac aagatccata aatatattgg tataaaaccc aatacttcca
     2041 gctacagtct taggagtgga attgattatt gtgtcatctc ggatataaac agagacacca
     2101 ggaaaaaaat gtgatgttct actattcagc cctgactgat tcttttgaag aatcggctta
     2161 tacaggctac agtaatatta tgccccttga tgagtatgtg gttttgttat atccggacat
     2221 aaaaaaaaca gtaatatatg accctgtcag gggaggagag gtcgctgtta atgcataccc
     2281 cgcgtcgggt aatcatattc agacaaccag acagagtgtg gtggtttatt ctgaccatac
     2341 cttttacgct tacagccggg tcaaaaataa ccaggcaaca gcacagttcc cggagaatcc
     2401 gatatttcct ctgttgagag agtctgataa agtggttttc atacatggag
//
LOCUS       DAOSPX010000418         2448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_39093_length_2448_cov_4.753030, whole genome shotgun sequence.
ACCESSION   DAOSPX010000418 DAOSPX010000000
VERSION     DAOSPX010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39093_length_2448_cov_4.753030"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..1099
                     /locus_tag="PLZ75_13935"
     CDS             335..1099
                     /locus_tag="PLZ75_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73541.1"
                     /translation="MKKYIYLTSTPEALVASMLPPEGFGMYLSTGTKKRNRGQNIFFE
                     VDLDQIGNIFDMESLEKRCVAKEDGSPKSSVYLSVYRVLEMVPTSALKSLYLTTDHGC
                     VLELKKAPYDSKRESVNTLHLYQELCPVTPLVVSDMTPSAFLKKLTDGSIPITLPKLF
                     FVELKLGELAANPLGGSAEHLPYPNIGHMRDCLEILRGEYEKHMKTVQRVFSGTLLYR
                     TIESGFYVGSKDEIVFYPYPTIKDLEDINYEFFRDI"
     gene            1254..>2448
                     /locus_tag="PLZ75_13940"
     CDS             1254..>2448
                     /locus_tag="PLZ75_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOS73542.1"
                     /translation="MISSLFWIVPVAALTALLFAWIFYRNMKAQEEGTDRMIEIATYV
                     REGAMAYLRRQYGVVTKVFLILVVLLLILAYFGIQNPFVPVAFLTGGFFSGLCGYLGM
                     RTATYASNRTAWAASKSLNQGLKVALRSGAVMGLVVVGFGLLDIALWFLILNGWVFTP
                     EHMAAGWKFAGLTFVHPGTTEHEKLIEITAVMLTFGMGASTQALFARVGGGIFTKAAD
                     VGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYAGSILATAALGA
                     ALPGVTGDLQIKSVIAPMLVSAIGIFLSIAGIYLVRTKESATPKALLHALLLGTGGSS
                     LLILVVLAIMAAADWITWGVFGATVSGLLAGVIIGQSTEYYTSDGNKPTQAIAKQAQQ
                     GPATVI"
BASE COUNT          648 a          543 c          568 g          689 t
ORIGIN      
        1 attaatattt tacagggcct ttaatttctg cagtaataat cagatcaatc cggcatcctt
       61 tgactgcagt tcttcatagc cgtaaaaacc ccgcaaatgt aataaatggc ggcatgaaaa
      121 aaaacataat cgttcagctt aacatttcat tacagcttat gcaattatgt aactgactaa
      181 gccagaattt cagggaagcc tgacaggaaa cagatccgga atctgatttg taatcatctg
      241 aaaagatatg tatatttgat taagaacctc gtttaattct ctttattcat gtttctccgg
      301 aaacgtatta ttcatcttct taacagctag agccatgaaa aaatacatct atctaaccag
      361 tacccctgaa gcgcttgttg cttcaatgct tcctcccgag ggattcggaa tgtatctttc
      421 taccggtacc aaaaaacgga acaggggaca gaacatcttt ttcgaggtgg accttgatca
      481 gatcggaaat atatttgata tggaaagtct tgagaaaaga tgtgtcgcaa aagaggacgg
      541 cagccctaaa agctctgttt atctgtcggt atacagagtt cttgagatgg ttcccacatc
      601 tgccctcaag agtctttacc tcactacaga tcatggttgt gttcttgaac tgaaaaaagc
      661 cccgtacgac agtaaaagag aatcagtaaa cactcttcat ctctatcagg aattgtgtcc
      721 tgtcaccccg ctggtggtat ccgacatgac tccttccgca tttctgaaaa aacttaccga
      781 cggatctatt cccatcactc ttccaaagct gttttttgtt gaactgaagc tgggagagct
      841 tgccgccaat ccgctcggcg gttctgcgga acatcttcct tatcccaata tcggacacat
      901 gcgggattgc ctcgagattc tcagggggga atatgaaaaa catatgaaaa cggttcagag
      961 ggttttttcc gggacacttc tgtacaggac aattgaaagc ggcttctatg tgggttcgaa
     1021 ggatgaaata gttttttatc cctatcccac aataaaggat cttgaagata tcaattatga
     1081 attcttcagg gatatctgat ccggcttttt caaccggcat ggtatccgga atattttcac
     1141 tggacagtca aatagatacg gtttttagtt tggattaatc aaaaactaat ttagttttgt
     1201 acccgaattt ttttcgtatc atacacaaca aaatttaatt aaaaactgat atcatgatta
     1261 gtagtctttt ttggattgtg cccgtagctg ctcttacggc cctgctgttt gcatggatct
     1321 tttaccggaa catgaaagca caggaagagg gtacagacag gatgatagag attgcgacct
     1381 atgtcaggga aggtgcaatg gcatatctca gacgccagta cggtgttgtt acaaaggtat
     1441 tcctgatcct tgtcgtactg ttactcatac ttgcatattt tggaattcag aatcctttcg
     1501 ttcccgttgc ctttcttacc ggggggttct tctccggttt atgcggttat cttggcatga
     1561 ggactgcaac ctatgcttcc aacagaactg cctgggcagc ctcaaaatcg cttaaccagg
     1621 gactgaaggt ggctctcagg agcggtgccg taatgggact cgtagttgtt ggattcggac
     1681 tgcttgatat tgcactgtgg ttccttatac tcaacggatg ggtattcacg ccggaacaca
     1741 tggcagccgg ctggaaattt gcaggactga catttgttca tccgggaact actgaacatg
     1801 agaaacttat agaaataaca gcagtgatgc ttacattcgg catgggtgca tcaacccagg
     1861 cactgttcgc acgtgtgggc ggtggtatct ttacaaaagc cgcggacgtg ggtgctgacc
     1921 tggtcggcaa ggttgaagca gggattcctg aggatgatcc gcgcaacccg gccaccattg
     1981 ctgataatgt cggtgacaac gttggtgatg ttgccggaat gggtgcagac ctttatgaat
     2041 cctatgccgg ttcaattctt gcaacagcag cactgggtgc tgctcttccg ggagtaaccg
     2101 gagatctgca gataaaatcg gtcattgccc cgatgcttgt ttcggcaata ggcatattcc
     2161 tgtcaattgc cggcatttac cttgtcagaa ccaaggaatc cgccactccc aaggcactgc
     2221 tgcatgccct tcttctcggt acgggaggca gctccctgct gatcctggtt gttctggcca
     2281 ttatggcagc agccgactgg ataacatggg gtgttttcgg agcaacggtt tccggactgc
     2341 tggcaggagt aatcataggc cagtcgacgg aatactatac ttctgacggc aacaagccga
     2401 cacaggctat tgcgaagcag gcacaacagg gtccggctac tgtaatta
//
LOCUS       DAOSPX010000419         2442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_39243_length_2442_cov_3.058232, whole genome shotgun sequence.
ACCESSION   DAOSPX010000419 DAOSPX010000000
VERSION     DAOSPX010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39243_length_2442_cov_3.058232"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLZ75_13945"
     CDS             <1..241
                     /locus_tag="PLZ75_13945"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="catalyzes the formation of alpha-D-galactose
                     1-phosphate from D-galactose in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOS73543.1"
                     /translation="YEVTGIELDTLVYEGRKLPGVIGTRMTGAGFGGCTVSIVKTKDT
                     KDFMAKLARIYTEITGLIPDFYQPAIGNGAGKLPC"
     gene            complement(254..>2442)
                     /locus_tag="PLZ75_13950"
     CDS             complement(254..>2442)
                     /locus_tag="PLZ75_13950"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS73544.1"
                     /translation="YKVYNGLYSGEWQEGIRTMFRDKYLFDLDRFDDSLYERSDLGWI
                     RNSYLIILQTAWDREFYDSQTGKYTYPELLKKSIGEFGRLDVYGLAATWPVQVLDRRS
                     QQDMYRNLPGGISQLRSFVNLGRSYGTRFFVACNPLEKRTEKENPRRGMTELVRDLDA
                     DGVMLETGAGSGFEGLQAAADSIRSGVVIYPSGMAGVREMQKIISGRVHNSIFLSPEL
                     NLSKFIKPEYAIFRVCDVGEDVLHREIAVSFFNGYGTELNMFRPGGRNEDFTADKEFL
                     AGTTFLLRQNNDAFTDRNWTPLIETAADNIFVNRWHSGDKTLYTVLNLRPEGYSGRLF
                     GVSGKKGYHYVSLWHHENLEPGEMNGRLYAVARTDAWPSAWAGTRREGSVDCIAEFPD
                     ILKSGLKGGDSLSINSPGRGLVTVWKGNPSYNTEKRDLKLTGDTTISLKSLFGGWFEG
                     KIVVQLTENNILKDENVINVSGGRPWPVSRINKSAGAVSVPSDMLLVPESTFTFDVKL
                     SGAFIHWSGTGSRTVTVDTFLIDKYPVTNAQYYEFISRTGYRPADTTRYLRHWSSGIY
                     KQGQDKYPVVYISYEDAQAYAQWAGKRLPSEAEWQLAAQGTDKRKWPWGNEFHATYCN
                     NAFERPTPVDAFPKGQSPYGVMDMVGNVWQMTNDMYFNGSNYFIIIRGGSFHNPGSGN
                     RYASGGPQPLDRTQILLRVSPGFERSETLGFRCVRDIDKKRFRPGK"
BASE COUNT          597 a          650 c          543 g          652 t
ORIGIN      
        1 ttatgaagta accggtattg aacttgacac tcttgtctat gaaggaagaa aacttcccgg
       61 ggtgatcgga acacgaatga ccggggcagg attcggggga tgtaccgttt ccattgtaaa
      121 aacaaaggat acaaaggatt tcatggcaaa actggccagg atctacacgg aaataacagg
      181 actgattccc gatttctacc agcccgcaat cggcaacgga gcagggaagc ttccgtgtta
      241 aatacgcggg cttttatttt ccgggtctga atcttttttt atcaatatcc ctgacacatc
      301 tgaaaccaag ggtttcactg cgttcgaacc cgggcgagac acgaagcagg atctgagtcc
      361 tgtcaagggg ctggggtccg ccggatgcat accggtttcc tgatccggga ttgtggaaac
      421 tgcctccccg tattatgatg aaatagtttg aaccgttaaa gtacatgtcg tttgtcatct
      481 gccacacatt gccaaccatg tccataaccc cataggggct ttgtcctttg ggaaaggcat
      541 caacaggagt gggcctttca aaggcattat tgcagtaagt ggcatgaaac tcattccccc
      601 acggccattt ccgtttatcg gttccctggg cagccagctg ccactcagct tccgaaggaa
      661 gccgttttcc ggcccattgc gcgtatgcct gcgcatcctc ataactgata tatacaacag
      721 ggtacttatc ctgtccctgt ttataaattc ctgatgacca gtgcctcaga tagcgtgtgg
      781 tatcagcagg ccggtagcct gtccttgata tgaattcata gtactgggcg tttgtcacag
      841 ggtatttgtc tatcaggaac gtgtcgacgg tcacggtccg gctgccggtt ccggaccagt
      901 gaataaaagc acccgacagc tttacgtcaa aggtaaaggt gctttcaggg acaagaagca
      961 tgtcggaggg cacggaaaca gctcctgcgg atttgtttat ccggctgacg ggccagggcc
     1021 tgccgccgct gacattgata acattttcgt ccttcaggat attgttttca gtcagctgaa
     1081 caactatttt tccttcaaac catcccccga aaagactctt caggcttatt gtcgtatccc
     1141 cggtaagctt cagatccctc ttttccgtat tatatgacgg attccccttc cagaccgtga
     1201 ccagtccccg tccgggtgaa tttatactta aggaatcacc tcctttcagc ccggatttca
     1261 gaatatccgg aaattctgca atgcaatcaa cggaaccctc ccttctggtt ccggcccatg
     1321 cggagggcca tgcatcagtt ctggcgacgg catataacct gccgttcatt tccccgggct
     1381 ccaggttttc atggtgccag agtgaaacat aatgatatcc tttcttcccg gataccccga
     1441 acagcctgcc ggaatagcct tccggccgca aattaaggac ggtatacaag gttttgtctc
     1501 ccgaatgcca cctgtttaca aaaatattgt cagcagcggt ttcaataagg ggggtccagt
     1561 tcctgtcagt aaaggcatca ttgttctgtc gcagaagaaa ggttgtccct gccaggaatt
     1621 ccttgtcggc tgtaaaatcc tcattccgtc ccccgggccg gaacatattg agttccgtgc
     1681 cataaccatt gaaaaaggat actgctattt cacggtgaag aacatcctct cccacatcac
     1741 atactctgaa aattgcatat tccggcttta taaacttgct gagattaagt tccggactta
     1801 aaaaaatgga attatgaacc cttcctgaaa taattttctg catttccctc acgcctgcca
     1861 taccggacgg atatataacc acaccgctgc gtatgctgtc tgcagctgcc tgcagtccct
     1921 caaaaccgct tcctgctcct gtttccagca tgaccccgtc agcatccaga tccctgacaa
     1981 gctcagtcat tcccctgcgg ggattctctt tttccgttct tttttccagg gggttacagg
     2041 caacaaagaa cctggtacca taggaacggc caaggtttac aaaactacga agctgtgata
     2101 ttccaccggg aagattcctg tacatgtcct gctggctcct cctgtcaagt acctgtacgg
     2161 gccatgttgc tgccaaacca taaacatcca gcctgccgaa ttccccgatc gattttttta
     2221 acagttccgg ataggtgtat ttaccggtct gactgtcata aaattctctg tcccaggctg
     2281 tctgaagaat gattaggtaa ctgtttctga tccagccaag atcacttctt tcatagagtg
     2341 aatcatcaaa cctgtccagg tcaaacaaat atttatccct gaacatcgtc cggatcccct
     2401 cctgccattc cccgctgtac aggccgttat ataccttata gt
//
LOCUS       DAOSPX010000420         2437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_39340_length_2437_cov_4.280017, whole genome shotgun sequence.
ACCESSION   DAOSPX010000420 DAOSPX010000000
VERSION     DAOSPX010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39340_length_2437_cov_4.280017"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2079
                     /gene="pnp"
                     /locus_tag="PLZ75_13955"
     CDS             <1..2079
                     /gene="pnp"
                     /locus_tag="PLZ75_13955"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOS73545.1"
                     /translation="GAVLLKQGKTMLLATVVAAKEPREDTDFMPLSVEYKEKYASSGR
                     IPGGFLKREARPSDNEVLISRLVDRALRPLFPEDFYHETFVTINLISAETGIMPDALA
                     GLAASAALAVSDIPFKGPISEVRVARINNQFVINPTLSELRESDMDLMVGATYDNILM
                     VEGEMKEVQESEMLEAIKVAHEAIKVHCRAQIEFAEELGRTVKREYKAVTDDEELRKK
                     VWDDTYDKCYQAAKSSTGSKQERTEAFDAILEEFLSGYAEDEEADEFLIRKYFHDVLK
                     ESARRLVLDENIRLDGRKPDEIRQIVCEIDPLPATHGSALFTRGETQSLTTVTLGTKL
                     DEKIIDEVLNQGSEKFVLHYNFPPFSTGEARPMRGVSRREIGHGNLAHRALKNMMPAD
                     EENPYAVRVVSDILESNGSSSMATVCAGSLALMDSGIKLRKAVSGIAMGLISDGNTDK
                     YAILSDILGDEDHLGDMDFKVAGTMDGITATQMDIKVEGLSFGILEKALDQAKAGRLH
                     ILGIMNETLSESRPELKPHAPRIEMLSIPKDMIGALIGPGGKVIQEIQRETGATIIVE
                     EINGRGDVNVFGENKEVISAAVDWIKRIVAVPEIGQVYRGKVKTIVQFGAFVEILPNK
                     DGLLHISEIDWKKINKVEDVLKEGDEVEVKLMEIDQKTGKLKLSRKALLPRPEKPDHE
                     HRPHRNHRQA"
     gene            2131..>2437
                     /locus_tag="PLZ75_13960"
     CDS             2131..>2437
                     /locus_tag="PLZ75_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOS73546.1"
                     /translation="METRYNYIVIEGNIGAGKTTLAGKIAGEFNARLILEHFADNPFL
                     PKFYEDPEKYSFPLELSFLAGRYRQLKEELESPDLFSSFTVADYYFTKSLIFAASTLR
                     "
BASE COUNT          726 a          527 c          630 g          554 t
ORIGIN      
        1 ggcgctgttc tcctgaagca gggaaagaca atgttgctgg caaccgtggt tgcggcaaaa
       61 gaaccaaggg aagacaccga ttttatgcct ctttctgtcg agtataagga aaaatatgca
      121 tcatcaggcc gtattccggg aggttttctt aaaagggaag ccagaccttc agataatgaa
      181 gtgcttattt caaggcttgt tgacagggct ttacgccccc ttttcccgga agatttctat
      241 catgaaacct ttgtaacaat aaacctgatt tcagccgaaa ccggtataat gcccgatgca
      301 cttgccggac ttgcagcttc tgcagcgctt gctgtctcag atataccctt caaaggcccg
      361 atttcggaag taagagtggc acgtatcaat aatcagtttg tcatcaatcc cacactgagt
      421 gagctcaggg aatcggacat ggacctcatg gtaggtgcca cctatgacaa tatactgatg
      481 gtggaggggg aaatgaagga ggttcaggag tcggagatgc tggaggccat taaagtcgca
      541 catgaagcga taaaggttca ttgcagggcc cagattgaat ttgcagaaga actcggaagg
      601 accgtcaagc gcgaatataa agccgtgaca gatgacgagg aactccggaa aaaagtctgg
      661 gatgatacat atgacaagtg ttaccaggct gcaaaatcat caacaggcag caaacaggaa
      721 cgcacggagg cctttgatgc catcctggaa gaattccttt ccggctatgc cgaagatgag
      781 gaagctgatg aattccttat aaggaaatat tttcatgatg tactgaagga atcggcgaga
      841 agactggttc tggatgaaaa tatcaggctc gacggcagga aacctgatga gatacggcag
      901 attgtctgtg agattgaccc tcttccggcc acccacggtt cagcattgtt cacaaggggg
      961 gaaacccagt ccctcacaac cgttacactc ggaacaaagc tggatgaaaa aataatcgat
     1021 gaagtactga atcagggaag tgagaaattc gtccttcatt acaatttccc cccgttttcc
     1081 acaggcgaag ccaggccgat gcgcggtgtc agccgcaggg aaataggtca tggcaatctc
     1141 gcgcaccggg cacttaaaaa catgatgccg gctgatgagg aaaatcctta tgcagtaagg
     1201 gttgtatccg acatacttga atctaatggt tcatcctcaa tggctacagt atgtgcagga
     1261 tcccttgccc tgatggattc ggggataaaa cttcgcaaag ctgtttcagg aattgcaatg
     1321 ggtctgataa gtgacgggaa tacggataaa tatgccattc tatcggatat cctgggcgat
     1381 gaagatcacc tgggagatat ggatttcaag gttgccggca caatggacgg aataacagcc
     1441 acccagatgg atatcaaggt tgaaggactg tcattcggga tactggaaaa ggcccttgac
     1501 caggcaaagg caggaagact gcatattctt ggaataatga atgaaaccct gtcagaatcc
     1561 cgtccggaac tcaaacccca tgccccgaga atagaaatgc tgtctattcc caaggatatg
     1621 ataggtgcac ttattggtcc gggaggaaaa gtgatacagg agatccagcg tgaaacggga
     1681 gccacaatta ttgttgaaga aataaacgga cggggcgatg taaatgtatt cggtgaaaat
     1741 aaggaagtca tttccgcagc cgtcgactgg ataaaaagga ttgtggcagt gccggagatc
     1801 ggacaggttt acaggggaaa agtgaagact attgttcagt ttggagcttt cgtggaaata
     1861 ctgccaaaca aggatggcct gctccacata tcggaaatcg actggaagaa aatcaataaa
     1921 gttgaggatg tactgaagga gggtgacgag gtagaagtaa agctgatgga aatagatcag
     1981 aaaaccggaa agcttaaatt atcccggaaa gctcttctgc cgcgtcctga gaaacccgac
     2041 cacgaacacc ggccgcacag gaatcacagg caggcctgat ttattttccg gatttccttt
     2101 caggagcgga aaaggctaaa gtcggcaata atggaaacca ggtacaatta tattgtaatt
     2161 gaaggcaata taggagccgg aaaaaccact cttgccggaa agatagccgg agaattcaat
     2221 gcccggctga ttctcgagca ttttgccgac aatccctttc ttcccaaatt ctatgaggat
     2281 ccggaaaaat attcctttcc cctggaactt tccttcctgg ccggccgtta cagacagctg
     2341 aaggaggaac tggagtcacc tgacctgttc agctcattca cggtggcaga ctattatttt
     2401 accaaatccc tgatctttgc cgcctcaacc ctcaggg
//
LOCUS       DAOSPX010000421         2437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_39345_length_2437_cov_3.434929, whole genome shotgun sequence.
ACCESSION   DAOSPX010000421 DAOSPX010000000
VERSION     DAOSPX010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39345_length_2437_cov_3.434929"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /locus_tag="PLZ75_13965"
     CDS             <1..1617
                     /locus_tag="PLZ75_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73547.1"
                     /translation="KRAPLDAWFYLARAYMINNDFDKSLATFRKFKALAQETRSGRGI
                     ENLDFTDQQIQACENAMEYAKDPVPVSKRMLGEGFSQGAMNENPVVSFDGNTIAYTER
                     RGLANVIYFSRKINGRWQTPVEITSQLNAGEDCSTCSLNNDGTELFLYKNDDYDGNIY
                     SSKFIDDNWTAIRKLNKNINTKYYESHAAVSADGKRLYFTSNREGGFGGLDIYVSELD
                     ATGDWGPPANLAPVVNSPYNEDNPFITGNDSLLYFCSEGHNSMGGFDNFRSRLSASGW
                     DKPENLGSPINSTDDDKFFHPADNGIYAYYSMKTDYKKRDIFFLGINGPDVNQIYEMR
                     GHIIPGDTASGTGGRYYVHIMNRISGETLYRTSPDRLTGRYSLTVAPGEFRIIFSGEG
                     YLPSAIDTAIIADSPDMLINLGDIVLMRDSSLIEAEYERINLAGIPSVSSVDSSLLVR
                     NLNVLNITDGSEEYGVLYYTVQVMALHNPIDISYFRYIADIQVMYNEKDKFYRYTTGQ
                     FRTKDEAGNWRHELIRRGYPNEIFIKKVLR"
     gene            1674..>2437
                     /locus_tag="PLZ75_13970"
     CDS             1674..>2437
                     /locus_tag="PLZ75_13970"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS73548.1"
                     /translation="MLNSQEITLKEFARSVQSADVVFYYDKDYKKAASLYEKLHEAFP
                     DNSNIQAKLGMCYLRLDGKKAEALRLLESASGNVAASAREYTETGQKAPLSTFRYLAE
                     AYHINDNLEKAVSLYTDLKNQLGNSEDDMEMADYYDLQIRDCRYAMEAKKKPVRVLSE
                     LFTPWMADYPGAMNPVLSRNDSVFVFTWRVEGKNRIMCSYKSGEWEEPTDITRELGGL
                     DRLYTNSITGDGRFLVLYIDDGDDGNLYFSERKGPA"
BASE COUNT          724 a          558 c          591 g          564 t
ORIGIN      
        1 aaaagagctc cgcttgatgc atggttctat ctcgccaggg cttacatgat caataacgac
       61 tttgacaaaa gccttgccac cttcagaaaa ttcaaggccc ttgcacagga aacccggtca
      121 ggacgcggca ttgaaaacct tgatttcacg gatcagcaga ttcaggcctg cgagaatgcc
      181 atggaatatg caaaggatcc cgttcccgta agcaagcgaa tgctcggcga aggattcagc
      241 cagggtgcca tgaatgaaaa cccggtggta agcttcgacg gtaatactat cgcctacacc
      301 gaaaggcggg gcctggcaaa tgtgatatat ttctcaagga aaatcaacgg ccgctggcag
      361 acaccggttg aaataacatc acagctcaat gcgggcgaag actgttcgac atgctcgctg
      421 aacaatgacg gaacggaact tttcctttac aaaaatgatg actatgacgg aaatatctat
      481 tcctcgaaat ttattgacga caactggaca gccataagga aactaaacaa aaatatcaat
      541 acaaagtact atgaatctca cgccgctgtc tctgctgatg gtaaaagact gtatttcacc
      601 agtaacaggg aggggggatt cggaggactt gacatatatg tctcggaact tgatgctacg
      661 ggcgactggg gaccgccggc caatctcgct ccggtggtaa acagccctta caatgaggat
      721 aatcccttta ttaccgggaa tgattcactt ctgtattttt gctcggaagg ccacaattca
      781 atgggcggat ttgataattt cagaagccgg ctcagtgctt ccggttggga caagcctgaa
      841 aatctgggtt cgccaatcaa ttcaaccgat gacgataagt ttttccatcc ggcagataac
      901 ggcatatatg cctattattc catgaagacc gattataaga aaagagatat cttcttcctt
      961 ggaataaacg gccccgatgt caatcagatc tatgagatga ggggacatat catacccggc
     1021 gacactgcct ccggtaccgg cggcagatat tatgtgcata tcatgaacag gatatcgggg
     1081 gaaacacttt acagaacctc tccggacagg ctgacaggcc ggtacagtct tacagtggct
     1141 ccgggggaat tcagaatcat attttccggc gaaggatacc tgccctcggc aattgatact
     1201 gcaatcattg ctgacagccc cgacatgctg ataaacctgg gggatatcgt gctgatgaga
     1261 gattcctcac tcatcgaagc ggaatacgaa agaatcaacc ttgccggcat accatcggtc
     1321 agctcggtcg actcaagcct gctggtcaga aacctcaatg tcctgaatat aaccgacgga
     1381 tcagaagaat acggagttct gtattatacc gtgcaggtta tggctcttca caaccccatt
     1441 gatatcagct atttcaggta tattgcagat attcaggtga tgtacaatga aaaggacaaa
     1501 ttctacagat atacaacggg acagttcaga acgaaagatg aagctgggaa ctggaggcat
     1561 gagctgatca gaagagggta ccccaatgag atattcatta aaaaagtact aagataatga
     1621 accctttaac gaagagaatt tttagccttt ttctgctgat cagctgtttc accatgctga
     1681 attcgcagga aataacactg aaggagttcg caagatccgt tcagagtgca gatgttgtct
     1741 tctactatga caaggactat aagaaagctg cttcgctcta tgaaaaactt catgaagcct
     1801 ttcccgataa ttccaatatc caggcaaagc ttggcatgtg ctacctgaga ctggacggga
     1861 aaaaagctga agccctgagg cttctcgaat cagcctccgg aaatgttgcc gcctcggcaa
     1921 gggaatatac cgaaacaggc cagaaagcgc cgctcagcac cttccggtat cttgcggaag
     1981 cttaccacat taatgacaac ctggaaaaag ccgtttctct ttacaccgac cttaaaaatc
     2041 agctgggcaa cagtgaagac gatatggaaa tggctgatta ctatgacctc cagataagag
     2101 actgcagata tgcaatggag gccaaaaaga agccggtcag ggtgctttcc gaacttttta
     2161 ccccctggat ggctgattac ccgggagcaa tgaatccggt cctttcaagg aatgattcgg
     2221 ttttcgtgtt cacctggagg gtggaaggca aaaaccgtat aatgtgctca tataaaagcg
     2281 gtgagtggga agaacccacg gatataacca gggagcttgg cggtctcgac aggctgtaca
     2341 ccaattcaat aacgggagac ggccgtttcc tcgtgctgta tatcgacgac ggggatgacg
     2401 gaaatcttta cttcagcgaa agaaagggtc cggcatg
//
LOCUS       DAOSPX010000422         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_39740_length_2420_cov_3.628330, whole genome shotgun sequence.
ACCESSION   DAOSPX010000422 DAOSPX010000000
VERSION     DAOSPX010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39740_length_2420_cov_3.628330"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1013
                     /locus_tag="PLZ75_13975"
     CDS             45..1013
                     /locus_tag="PLZ75_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOS73549.1"
                     /translation="MTMYRFAAILSVFLLCTAGKSAAQADADPERIRPGYPVYSQYLH
                     NGLMINPAYAGSRDALSVLLSCRVQWMGIPDAPRIESVSLHSPLRNDKVALGLKAGFM
                     QYGVTKSQSIYGIYAYHIRLKTGKISLGLKAGTELSNTDYRGIPGITRPDPAFPETDK
                     SYIFPNAGAGVYYFTDKFFAGLAVPSFLFYRNTGNGHVQASHSFSEYDFVVSAGALLS
                     LSPVFMIKPSLLIDYSMNKSDHLNQFDVNCNFIFSDLVWLGASWRITEQVAVGHLQIQ
                     INPRLMAGLSYDYPAGRMNSFSRGSSEIILRYEFGSRISAANPKYF"
     gene            1016..>2420
                     /locus_tag="PLZ75_13980"
     CDS             1016..>2420
                     /locus_tag="PLZ75_13980"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOS73550.1"
                     /translation="MRRTLRYSISITCLLMVAGSVQSQQPSLYRVKRMSFNDPIFSEM
                     SPVIIRDGIIFCSDRRFSAVKDRKSFEGRRVYNLYKAMRKDSTEYGKPVMIRGERSGL
                     FNIGPLCFAPDGKSVWFTSEVETGRAAKKRGYKNHSGIFRAELNGDELVSLSAFPYNS
                     TEYNIAQPSLSSDGKYLYFASDMPGGEGKSDIWVSELVDGQWSRPVNPGPGINSTGRE
                     SFPFIHPSGRLYFSSDRDGGYGKLDVYYTTLSDGVWADPVLLPEPLNSPENDFAIVAA
                     DDMQSGFFSSDRRINDDIYSFTSTIIRKSVCDTLIENNYCYELVEENAVKHDSIPFRY
                     EWHFGDGSKGTGPSVIYCYPGPGTYMIRLDVVNLITGEILYNEKTFSLEITDIEQPYI
                     SAADTASAGRNIFFSAEKTNLPGWNIAEYYWNFGDETVAGGMKVSKSYLKPGTYNVQL
                     IVRTEPGEAGIIREACIC"
BASE COUNT          654 a          535 c          609 g          622 t
ORIGIN      
        1 gaaggcggaa caacggcaag aatatgtaac agtcagcgaa aggaatgaca atgtacagat
       61 ttgcggcaat attatcagtg tttcttctgt gtacagcggg aaaatccgct gcacaggctg
      121 atgctgatcc ggaaaggatc aggcccggtt atcccgttta cagtcagtat ctccataacg
      181 gcctgatgat caatcccgca tatgcaggat cacgggatgc actcagtgtt ctcctttcct
      241 gccgggttca gtggatgggc ataccggatg ctccccgcat cgagtctgta agtcttcatt
      301 cacctctcag aaacgacaag gttgccctgg ggctgaaggc aggtttcatg cagtacggag
      361 tcactaaatc acagagtatt tacgggatat atgcatatca cataagactg aaaacgggaa
      421 aaatatctct tggactgaaa gccggaacgg aactgtcaaa taccgattac agagggatac
      481 cggggataac aagacccgat cccgctttcc ctgaaacgga taaatcctat attttcccga
      541 atgccggtgc cggtgtatat tactttacag ataagttctt tgccgggctt gcggttccgt
      601 ctttcctatt ttacaggaat acgggaaacg ggcatgtgca ggcttcccat tcattcagtg
      661 aatacgattt tgtagtttct gcaggagccc ttttatcgtt atctccggtt ttcatgatca
      721 agccctcgct gctgattgac tattccatga ataaaagcga tcatctgaac cagtttgatg
      781 tgaactgcaa ttttattttt tccgaccttg tgtggctggg agcatcctgg agaatcacag
      841 agcaggtagc tgtcggtcat ctgcagatac agataaaccc gcggctcatg gccggactgt
      901 catatgatta tcctgcagga agaatgaatt ccttttccag gggatcgagt gaaattattt
      961 taagatatga attcgggtcc aggatcagtg ccgcgaatcc gaaatatttt tgattatgcg
     1021 cagaaccctg aggtatagca tcagcattac atgccttctt atggtggccg gctccgttca
     1081 gtcgcagcag ccatcccttt acagggttaa aaggatgtca ttcaatgatc ccattttcag
     1141 cgaaatgtcg ccggtaataa tccgggatgg cattattttc tgttccgaca ggcgtttcag
     1201 tgcggtaaaa gaccgcaaat cctttgaagg cagaagggtt tataacctgt acaaagccat
     1261 gaggaaagac agtactgaat acggaaaacc tgtcatgatc cgcggtgagc gcagcggact
     1321 tttcaatatc ggcccccttt gctttgcccc ggatggaaag agtgtgtggt ttaccagtga
     1381 ggtggaaacg ggcagagctg caaaaaagcg cggttataaa aaccacagcg gcatattcag
     1441 ggctgagctg aatggtgacg aacttgtgtc attaagcgcc tttccctata acagtacgga
     1501 atacaatatt gcccagccgt cactgagcag tgacggaaaa tacctttatt ttgcctccga
     1561 catgccggga ggggagggaa aatccgatat ctgggtcagt gaacttgttg atggccagtg
     1621 gagcagaccc gtcaaccccg gtcccggcat caacagtacc ggcagagaga gcttcccgtt
     1681 catccatcct tcggggaggc tttatttttc atcggaccgt gacggaggat acggcaagct
     1741 tgatgtctat tacacaacct tgtcggacgg agtatgggca gacccggtac tgcttcccga
     1801 accccttaat tcccctgaga atgattttgc tattgttgcc gcggatgaca tgcagtcggg
     1861 atttttttcc tccgacagga ggataaatga tgacatttat agttttactt ccacaataat
     1921 cagaaaatca gtttgtgaca cactgattga gaacaattac tgttacgagc ttgtagaaga
     1981 aaatgcagtt aagcatgaca gcataccttt tcgctatgaa tggcatttcg gtgacggcag
     2041 taaaggaaca ggcccttctg taatatactg ctacccgggc cccggaacat acatgatccg
     2101 tctggatgtt gttaatctca taaccgggga gattctttac aatgaaaaaa cattcagtct
     2161 tgagataacg gatattgaac agccctatat atcggctgcg gatacagcca gtgcagggcg
     2221 gaatatattt ttcagtgcgg aaaagaccaa tcttcccgga tggaatattg cggagtacta
     2281 ctggaatttc ggggacgaga ccgtggcggg cggcatgaag gtgagcaaga gttatttaaa
     2341 acccggaaca tataatgttc agctgattgt aaggactgaa cccggagagg caggaataat
     2401 cagggaagcg tgtatatgca
//
LOCUS       DAOSPX010000423         2414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_39878_length_2414_cov_3.600678, whole genome shotgun sequence.
ACCESSION   DAOSPX010000423 DAOSPX010000000
VERSION     DAOSPX010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39878_length_2414_cov_3.600678"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1555
                     /gene="gmd"
                     /locus_tag="PLZ75_13985"
     CDS             398..1555
                     /gene="gmd"
                     /locus_tag="PLZ75_13985"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOS73551.1"
                     /translation="MSKTALITGITGQDGAYLAEFLLKKGYIVHGIKRRSSLLNTDRI
                     DHLYQDPHTENRSLILHYGDLSDSMNITRIIQECRPDEIYNLGAMSHVKVSFDVPEYT
                     ANTDGIGTLRILEAIRLLGLKDVTRFYQASTSELYGLVQEVPQTEKTPFYPRSPYSVA
                     KLYAYWITVNYREAYGLHASNGILFNHESPIRGETFVSRKITRGLAKIVLGLQNTLFM
                     GNLSAKRDWGHAKDYIQAMYLMLQQDEPDDYVIATGITTEVREFIRRSFETVGVTLVF
                     EGKREKETGSIARVNDEMFAEKVGEEYLENFRMRIKKDPVAVRVDPAYFRPTEVELLI
                     GDPTKAKSRLGWKPEYDLDKLIEDMIISDLVLMKKEDYLREGGYEILNYFE"
     gene            1559..>2414
                     /locus_tag="PLZ75_13990"
     CDS             1559..>2414
                     /locus_tag="PLZ75_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOS73552.1"
                     /translation="MNRLSKIYVAGHAGLVGSAITRNLRSEGYVNLVFRTFSELDLTN
                     QQAVEDFFSAEKPEYVFLAAALVGGIVANNTYRADFIYNNLMIQSNVIHACWKYGVKK
                     LLFLGSTCIYPKNAPQPLKEEYLLTSPLEYTNEPYAIAKIAGLKTCESFNIQYGTDFI
                     AVMPTNLYGAGDNYDLEKSHVLPALMRKIHLGKCLEKGDREAVIRDLKKNPVEGLGED
                     PADDEIITVLGKSGVEARAGFRNGIETESAKEVVVSVWGTGSPYREFMYSEDMARACI
                     YIMEKVSAS"
BASE COUNT          705 a          470 c          613 g          626 t
ORIGIN      
        1 ggacgagtca ggaaatatca gggcggtata tgcacagaaa ctctgatact attagtgtta
       61 tttgatctgg cccttgctgt aatacgggct ttcatattaa atgatcttgg catcagccgt
      121 cctattacac tgtgtggaaa tctgcaaagg ggagcagtaa gattgcttga tcctctgctt
      181 ctgcggaata gtttcttttt aacttaaatt tccggtttga gtgttgaatg gagttattgt
      241 catttcgaaa gccgggtaaa tgaatgctgt acacagaaaa cttgttgata attattttgt
      301 ttctacaata taatcgccgg aaaacttttg gaatgaaagg aatttttgtt tctttttgtc
      361 tggcattacg tataatataa aaacttaata ccgataaatg agcaagacag cattaattac
      421 aggtatcaca ggccaggacg gagcttatct ggctgaattt ctcctcaaaa aagggtacat
      481 tgtccatggt ataaagagaa gaagttccct tttaaacact gaccgtattg atcacctcta
      541 ccaggatccg cacacggaaa acagaagcct gatacttcat tacggcgatc tgagtgattc
      601 gatgaatatt acacgtataa ttcaggaatg ccgcccggat gagatataca acctgggagc
      661 catgagtcat gtgaaagtca gttttgatgt tccggaatat accgcaaaca ccgacgggat
      721 tggtaccctg cgtattctgg aggcaatacg cctgctgggc ctgaaggatg ttaccaggtt
      781 ttatcaggca agtacttcgg aattatacgg actggttcag gaagtgccgc agacggagaa
      841 gactcccttt tatccccgga gcccgtattc cgttgccaaa ctctatgcct actggataac
      901 cgtcaattac agggaagcct atgggctgca tgcaagtaac gggatactgt tcaaccatga
      961 atctccgata cgcggtgaga ccttcgtgtc gagaaagata acaagagggc ttgcaaagat
     1021 cgttctgggc ctgcaaaaca ccctttttat gggaaacctg tctgcaaaac gtgactgggg
     1081 acatgcaaag gattatatcc aggccatgta cctgatgctt cagcaggatg agcctgatga
     1141 ttacgttatt gccacgggca ttaccaccga ggtccgggag tttataaggc gctcatttga
     1201 aacagtggga gtcacactgg tttttgaagg gaagagggag aaggaaaccg gcagtattgc
     1261 ccgggtaaat gatgagatgt ttgctgaaaa ggtaggagag gagtatctcg aaaatttcag
     1321 gatgaggata aaaaaggatc cggtggctgt gagggttgat cctgcatatt tccgtccgac
     1381 agaagtggaa ctgcttatcg gggaccctac aaaagcaaaa tccaggctgg gctggaaacc
     1441 ggaatatgat ctggataaac tgatagagga tatgattatt tccgaccttg tactgatgaa
     1501 aaaagaggat tatcttcgtg aaggaggtta tgaaatactg aactattttg aatagtatat
     1561 gaacagattg tcgaaaattt atgtggccgg acatgccgga cttgtcggat cggcaataac
     1621 aagaaatctc aggtcggaag gatatgttaa cctggtattc aggactttca gtgaactgga
     1681 ccttacgaac cagcaggccg ttgaggattt tttttcggct gaaaaaccag aatatgtgtt
     1741 tcttgctgca gcccttgtgg ggggaatagt tgccaataat acctacaggg cagattttat
     1801 ttacaacaac ctgatgattc agagcaacgt gatccatgcc tgctggaaat acggggtgaa
     1861 aaaactgttg tttctcgggt caacctgcat ttatcccaaa aatgccccgc agccgctgaa
     1921 agaagaatac ctgcttacct ccccgctgga atacaccaat gagccttatg ccatagccaa
     1981 gatagcagga ctaaaaacct gtgagtcatt caatattcag tatggtacgg atttcattgc
     2041 agtaatgccg accaatctct atggtgcggg agataactat gacttggaaa aatcgcatgt
     2101 actgcctgca ctgatgagaa aaatccatct gggtaaatgc cttgagaaag gagacaggga
     2161 ggcagtcata agggatctga agaagaatcc ggttgagggg cttggagaag atcccgctga
     2221 tgatgagatc atcactgttc tgggaaagtc cggagtggaa gcaagagccg gtttccggaa
     2281 cggaattgag acggaatccg caaaagaggt agtggtttcg gtgtggggaa caggttcacc
     2341 ataccgcgaa ttcatgtatt ccgaagatat ggcccgtgcc tgtatctaca tcatggaaaa
     2401 ggtcagtgcc tcag
//
LOCUS       DAOSPX010000424         2411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_39951_length_2411_cov_3.955433, whole genome shotgun sequence.
ACCESSION   DAOSPX010000424 DAOSPX010000000
VERSION     DAOSPX010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39951_length_2411_cov_3.955433"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..557
                     /locus_tag="PLZ75_13995"
     CDS             51..557
                     /locus_tag="PLZ75_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73553.1"
                     /translation="MIEKPLAMEMDKLIRAVDAVKAAGTVVQVGTQLRSLPGITGARE
                     LFRSGKLGKLSRVEECRNSEQPYWYRYLKEVKQQDVDWKEFLSDRPMRPFNSDLYSGW
                     YGYADFSHGPISGFGSHFIDMVHYITGAEFPESCVYLGEFLPGRMNISSHFRIVYRQH
                     GSIRKDLW"
     gene            1245..2282
                     /locus_tag="PLZ75_14000"
     CDS             1245..2282
                     /locus_tag="PLZ75_14000"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HOS73554.1"
                     /translation="MHYDFEKIDRYIKGVADEKEREIIESLLSEGEKNLYLRNCLEKD
                     WNSLLSNEVPAGVDLAPILDRIHHLIRKNETQKPLKPVHKLLRIYMKVAAILLIPLLV
                     AGGLIYSYLAYKQQIIEGERVSARIFAPMGSRVSFSLPDGTRGMLNSGSRLDYSVPFT
                     SDRKVNLEGEAWFEVSHDEKHPFEITAGSSVVKVLGTSFNISAYPAENYIEMVLSSGK
                     VEYHNNISNENMILLPSERLVCHEGRTDKTVVDPAKYNAWTEGKLVFRGDPMAEVARR
                     IERWYNVNIQIADKELEKYSFRATFQDDPLEDVLKFLSMTSPIRFKISKRKSLPDGTF
                     TKEEVTIYKKN"
BASE COUNT          751 a          479 c          537 g          644 t
ORIGIN      
        1 cagttgttta tgcagataaa ttgtaaatct aaaagtttaa tcggacaata gtgattgaaa
       61 aaccacttgc aatggagatg gacaagctta tcagggcagt ggatgcagtt aaggcagccg
      121 gaacagttgt tcaggttggc acacaattac gcagtcttcc gggaataaca ggagcccggg
      181 aactgtttcg ctcaggcaaa ctgggcaaat tatcgcgggt tgaagagtgc cgcaacagtg
      241 aacagccata ctggtacagg tacctgaaag aggttaaaca gcaggatgta gactggaaag
      301 aatttctgtc tgaccggccg atgagacctt ttaactcaga tctttattcg ggatggtatg
      361 gttatgccga tttcagccat ggaccaattt caggtttcgg ttctcacttc atcgacatgg
      421 tacattatat taccggagct gagttccctg aatcctgtgt atatctgggg gaatttttac
      481 ctggacggat gaacatcagt tcacacttcc ggattgtgta caggcaacat ggctctatcc
      541 ggaaggattt atggtaagca gttcaaataa tctgggacat ggaatgggaa gtgtccggaa
      601 cttttacggt gataagggaa tgctgaatgt cacaaactga aattcacccg tttacagcga
      661 caaaggttca ccgcttagag atggaagcat caaaggggaa aatcccgtta acccggttga
      721 caggccggat cattttcttg actggttgca atgtatacgg tcgggaggaa cacctcatgc
      781 tccgattgaa gccgggtatc agcatgccgt agctgttctt atggccgcag tatctcataa
      841 aactgggcga aaagctattt acgatcctgt taaacgtaaa atccatacag catgaaaaat
      901 caaaaaataa cccgaaggcg tttcacagaa aagatgataa tttaaaaatc atattaggat
      961 gttgtaatta tacagcttac aaaggtgtgg atatcagtgt aaaacctcac ggaccattaa
     1021 actccacagg acgggaatgc cgtccgctaa ttgaaaatgt cggacacagt tacagctgat
     1081 cggtcggtta ctaccttcct cgacttccga atccgctttt gcgggatttc gtcgcttcgc
     1141 tcctcaactt cggacttctt tcggactgat ctttcaaaaa aaagcatttc ccatatgaga
     1201 tttaccttag ttatggatat aacattgaaa ctacatagaa tttgatgcac tacgattttg
     1261 aaaaaatcga ccgctatatt aaaggtgtag cagatgagaa agaaagagag attattgaat
     1321 ccctgctttc tgaaggagaa aaaaaccttt atttacgtaa ttgtcttgaa aaggactgga
     1381 attcactatt aagtaatgaa gtacctgccg gagttgatct ggctcccata cttgaccgta
     1441 tccatcattt aatcagaaag aatgaaactc agaaaccgtt gaagcctgta cataagctcc
     1501 ttcgtattta tatgaaagtc gccgcgattc tgcttatccc gctgttggtc gcgggaggac
     1561 tcatttatag ttatctggct tacaaacaac agataattga aggggagcgt gtttcagccc
     1621 gtatttttgc accgatggga tcgcgtgttt ctttcagtct cccggacggg accaggggaa
     1681 tgcttaacag cggatcacgt cttgattatt ccgttccttt cacttctgac cgaaaagtta
     1741 atcttgaagg agaagcctgg tttgaggtca gtcatgatga aaagcatcct tttgaaataa
     1801 ctgccggcag ttcagttgtg aaagttcttg gaacaagctt taatatcagt gcatatcctg
     1861 ctgaaaatta tatcgaaatg gtgcttagtt ctggcaaagt tgaatatcac aacaatattt
     1921 ccaatgaaaa catgatcctt ttgccatcgg aaaggctggt atgtcatgaa ggcagaaccg
     1981 ataaaacggt agtggatccg gcaaaataca atgcctggac agaagggaag cttgttttca
     2041 ggggagatcc gatggcagaa gtggcaagaa gaattgagcg atggtacaat gtaaatatcc
     2101 aaattgcaga caaagagctt gaaaagtact cattcagggc aacctttcag gatgatccgc
     2161 ttgaagatgt cctgaaattc ctatcgatga cctccccaat ccggtttaag atttcaaaac
     2221 gaaaatcatt accggatgga acctttacca aagaggaggt aaccatatat aaaaagaatt
     2281 gatctgtata acccttaaac tattatctat gaaacccatc cacagtccct gaaaaaagag
     2341 aaaaaaaagg aaatcgcgcc aacgatttcc caaaaaacat cttaacgaca ttttttaact
     2401 taaaacctta c
//
LOCUS       DAOSPX010000425         2375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_40785_length_2375_cov_4.387931, whole genome shotgun sequence.
ACCESSION   DAOSPX010000425 DAOSPX010000000
VERSION     DAOSPX010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40785_length_2375_cov_4.387931"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1390
                     /gene="secY"
                     /locus_tag="PLZ75_14005"
     CDS             59..1390
                     /gene="secY"
                     /locus_tag="PLZ75_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOS73555.1"
                     /translation="MRLIQTIRNIFKIEDLRARLIYTFGIILLYRFGKYITLPGIDPS
                     QLEGLRHQTSSGIMGLLDMFSGGAFSQASIFALGIMPYISASIVVQLLGIVFPYFQKL
                     QKEGESGRRKMNQFTRILTVFILVLQAPTYLVNLHAQLPASAFIMTGVFFQVSSVIIL
                     TAGTMFVMWLGERITDKGIGNGISLIIMIGIVARMPANFVFEAGARMNGAGGAVALVV
                     EMVVLFVVILGTVLLVQGTRRVPVQYARRIVGNKQYGGVRQYIPLKVNAAGVMPIIFA
                     QAIMMLPIIIAGYSSNPTSGFVVAFSNMYGFWYNLVTAILIILFTYFYTAVTINPVQM
                     AEDMKKNGGFIPGIKPGRKTVEFFDTIMSRITLPGSIFLAIIAILPAIAVQVGITPQF
                     AQFYGGTSLLILVGVILDTLQQIESHLLMRHYDGLMKSGRVKGRSGAVTSI"
     gene            1410..2186
                     /gene="map"
                     /locus_tag="PLZ75_14010"
     CDS             1410..2186
                     /gene="map"
                     /locus_tag="PLZ75_14010"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOS73556.1"
                     /translation="MLHYKTDEEVGLLRKSNLLVSRALAEVAGHIRPGVTTLYLDSIA
                     ETFIRDNNAIPAFKGYGGFPNSLCTSVNAEVVHGIPSDYVLKEGDIISIDCGVVLNGW
                     YGDGAYTFAVGRISDELKRLLEYTRASLEEGVRAAVAGNRIGDISSAVQTMAESGGYS
                     VVRELVGHGLGKKLHEAPEVPNFGKRGSGPKMKKGLVICIEPMINLGRKETVRLNDGW
                     TIRTADGRPSAHFEYAVAVDKGRADVLTTFQFIEDVLNKM"
     gene            2191..>2375
                     /gene="infA"
                     /locus_tag="PLZ75_14015"
     CDS             2191..>2375
                     /gene="infA"
                     /locus_tag="PLZ75_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOS73557.1"
                     /translation="MAKQPSIEQDGTIIEALSNAMFRVELENGHVITAHISGKMRMHY
                     IKILPGDKVKVEMSPYDL"
BASE COUNT          625 a          502 c          632 g          616 t
ORIGIN      
        1 aaatcggctg tacaggcgat tgaggccaat aacggaactg ttgtaaaact ttgatttcat
       61 gagactgatt cagaccataa ggaacatatt caagatagaa gacctgcgtg ccagactgat
      121 ttacaccttc ggcatcatac ttctttacag gtttggcaaa tatattaccc tgccgggtat
      181 tgatccctca cagcttgaag ggctgaggca tcagacctca tccggaataa tggggttgct
      241 tgatatgttt tcgggtggtg cattctccca ggcttcaata tttgccctgg gaataatgcc
      301 gtatatatca gcctctatcg ttgtccagct tctcgggatc gtgtttccct attttcagaa
      361 actgcagaag gagggcgaga gcggcaggcg taaaatgaat cagttcacca ggatactgac
      421 cgtgttcatc ctggttcttc aggctcccac ctatcttgtc aacctgcatg cacagcttcc
      481 ggcctcggca tttattatga caggcgtgtt cttccaggta tcgtcagtga tcattctgac
      541 tgccggaaca atgttcgtca tgtggctcgg ggaaaggatc accgacaagg ggataggcaa
      601 cggaatttcg ctcataatca tgattggcat tgttgcaagg atgcctgcaa atttcgtgtt
      661 tgaagccggt gcaagaatga acggtgcagg cggtgccgtg gccctggtgg tggaaatggt
      721 ggtcctgttc gtggtaatac tcggaacagt gcttcttgta cagggaacta gacgtgttcc
      781 ggttcagtat gcaagaagga tagtgggaaa caagcagtac ggcggagtaa ggcagtacat
      841 acccctcaag gtgaatgcag cgggagtgat gccgattatc tttgcccagg cgatcatgat
      901 gctgccgata atcattgccg gatattcatc caatccgact tccggttttg tggttgcatt
      961 ctccaacatg tacggtttct ggtataacct ggtgactgca atactgatta ttctctttac
     1021 atatttctat acggccgtta ccataaatcc tgttcagatg gctgaagaca tgaagaaaaa
     1081 cggaggcttt atcccgggta tcaagcccgg caggaaaacg gttgaattct ttgatacaat
     1141 aatgtcgagg ataacattgc cgggatccat tttcctggcg attattgcaa tcctcccggc
     1201 catcgcggta caggtgggaa taacacccca gtttgcacag ttttacgggg gaacctccct
     1261 gcttattctg gtgggtgtta ttctggatac ccttcagcag attgaaagcc atcttctgat
     1321 gaggcattat gatggcctga tgaaatccgg aagagtaaaa gggcgctccg gtgcggtaac
     1381 gtccatataa aactgtatat cgttctttga tgttgcatta taaaactgat gaagaggttg
     1441 gattgttgag gaagagcaat ttgctggtgt ccagagcact tgccgaagtg gccgggcata
     1501 taaggccggg ggttacaacc ctgtatcttg acagtatcgc cgaaacattc atccgtgaca
     1561 acaatgccat accggcattc aagggttacg gtggtttccc caacagtctc tgtacatccg
     1621 taaatgcaga agtggttcac gggatccctt cggattacgt gctgaaggag ggagatatca
     1681 tttccattga ctgcggagtg gtattgaatg gctggtacgg tgacggggcc tatacctttg
     1741 cggtgggcag aataagtgat gagcttaaaa gacttcttga atacaccagg gcatcccttg
     1801 aagaaggagt cagggcggca gttgccggca accgtatagg ggatatttca tcagcagtcc
     1861 agaccatggc cgaaagcgga ggctattcgg ttgtcaggga actggtgggc cacggactcg
     1921 ggaagaaact gcatgaagca ccggaagttc cgaatttcgg aaaaagggga agcggcccga
     1981 aaatgaaaaa aggactggtc atttgtattg aacccatgat taatcttggc aggaaggaaa
     2041 cggtaaggct gaatgacggc tggaccatcc ggactgcaga cggcaggcct tcagcacatt
     2101 ttgaatatgc ggtcgcggtt gataagggca gagcggatgt tttgacaaca tttcagttta
     2161 ttgaagacgt tttaaataaa atgtgataat atggctaaac aaccatctat tgaacaggac
     2221 ggtacgataa ttgaagcttt atcaaacgca atgttcaggg tagaacttga aaacgggcat
     2281 gttattaccg cgcacatctc cggtaagatg agaatgcact atatcaaaat cctgcccggt
     2341 gacaaggtaa aggttgaaat gtcgccctat gatct
//
LOCUS       DAOSPX010000426         2356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_41256_length_2356_cov_3.730117, whole genome shotgun sequence.
ACCESSION   DAOSPX010000426 DAOSPX010000000
VERSION     DAOSPX010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41256_length_2356_cov_3.730117"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLZ75_14020"
     CDS             complement(<1..246)
                     /locus_tag="PLZ75_14020"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOS73558.1"
                     /translation="MKLTLGFSPCPNDTYIFEAMVHGLIDTEGLEFDYFMADVEELNQ
                     QAFRGLTDITKMSCHAYSFAAGNYLILDSGSALGYKNG"
     gene            complement(243..1079)
                     /locus_tag="PLZ75_14025"
     CDS             complement(243..1079)
                     /locus_tag="PLZ75_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73559.1"
                     /translation="MNVLLVTATETEAAVFRKYAVNDSLRNASPDSPGLSGRYAPAEC
                     PERSARGGSAGSPDLPGNFNLSVMVTGIGSVATAWALTRRLCSGPRPDLVINAGIAGS
                     FRDSIRTGDVVMPLTDCFADAGVESAEGFLTLSEAGLQDPDSHPFTGGRINAYSCPAG
                     FQYPGPSVNRGERTLEPDSFLRLAAANVRPVRALTVNTVTGTAETAGRLMRKYDPDIE
                     TMEGAAFFYVCSREGIPFIALRAVSNRVGVRDRAGWNIPLALENLALLFEDLLKKTLL
                     KR"
     gene            1176..1589
                     /locus_tag="PLZ75_14030"
     CDS             1176..1589
                     /locus_tag="PLZ75_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOS73560.1"
                     /translation="MMIYLTRRERFSAAHRMFRQDWTDEKNMEVFGKCSNPRWHGHNY
                     ELFVTVKGEISEETGFIMNVGRLKEIIRARIIDRIDHRNINLEVDFMKGKTATTENLA
                     ISIWDELRPFIEQEGAKLHCVRICETENNSIEYYG"
     gene            1582..2229
                     /gene="folE"
                     /locus_tag="PLZ75_14035"
     CDS             1582..2229
                     /gene="folE"
                     /locus_tag="PLZ75_14035"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOS73561.1"
                     /translation="MAEKNESGKISELNVHNGYNRIESWDEESIRELSGMYYRVLKLL
                     GEDPDREGLRKTPERVAKALHFLMMGYSMDPCEIINSAKFREDYKQMVIVKNIDIYSM
                     CEHHMLPFFGQAHVAYIPDGYITGLSKIARVVEAYSRRLQVQERLTVQIRNTLQDCLR
                     PLGVAVVIEAQHMCMQIRGVQKQNSVTTTSAFTGIFLSNQNTREEFIHLIGSGLH"
     gene            2238..>2356
                     /locus_tag="PLZ75_14040"
     CDS             2238..>2356
                     /locus_tag="PLZ75_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HOS73562.1"
                     /translation="MKAYVFPGQGAQFPGMGKDLYEGSALAKEMFEKADRLLG"
BASE COUNT          671 a          554 c          593 g          538 t
ORIGIN      
        1 accgttttta tatcccaggg cacttcccga atccagaatg agataattac cagcagcaaa
       61 tgaataagcg tggcagctca tctttgtaat atccgtcagt cccctgaaag cctgctggtt
      121 aagctcctcc acgtcggcca tgaagtaatc gaactcaagt ccttcagtat caatcaggcc
      181 atgtaccatg gcttcaaaaa tataggtatc attcgggcag ggcgaaaagc ccagtgtcag
      241 tttcatcttt tcagcagagt ttttttaaga agatcttcaa atagcagggc aaggttttcc
      301 agtgcaagcg ggatgttcca gcctgcacgg tcacgtacgc cgactctgtt ggaaacggct
      361 ctcagtgcaa tgaagggaat cccctcccgg ctgcatacat aaaagaaggc tgctccttcc
      421 atcgtttcaa tatccggatc gtatttccgc atgagcctgc cggcagtttc cgcggtaccg
      481 gtaacggtat ttaccgtcag ggccctgaca ggccggacat ttgctgccgc cagcctgaga
      541 aagctgtcag gttcaagggt cctttcaccg cggttgacag aaggacccgg atactgaaaa
      601 cctgcagggc agctgtatgc attaatccgg cctccggtga agggatggct gtccggatcc
      661 tgaagaccgg cttctgaaag agtaaggaat ccttcagcac tttcaacacc tgcatccgca
      721 aagcaatccg taaggggcat caccacatcc cctgtcctga tggaatccct gaaacttcct
      781 gcaatcccgg catttatgac aagatccggt cggggaccgg aacagagccg cctggtgaga
      841 gcccaggctg tggcaacact tccgattccc gtgaccatta cgctgaggtt aaaatttccg
      901 ggaagatcag gactacccgc agaaccaccc ctggcgctac gctccggaca ttcagcggga
      961 gcatacctgc cagaaagccc ggggctgtca ggggaagcat tccggaggga atcattgaca
     1021 gcatactttc tgaatacagc tgcttcagtc tcagtggcgg taaccagtaa aacgttcacg
     1081 gtcttcgatc aaatagcatc agttcaacaa tattgagctt aaatatattt aaaaagatca
     1141 actttaatgg ttaatattgc agcttaaata actgaatgat gatctatctc acccgccgcg
     1201 aaagattcag tgcagcacac cggatgttca ggcaggactg gacggatgaa aagaacatgg
     1261 aagtgtttgg caaatgttca aatcccaggt ggcacggaca taattacgag ttgtttgtaa
     1321 cggtaaaagg tgaaatttca gaagaaaccg gatttatcat gaatgtgggc aggctgaagg
     1381 aaatcatccg ggccaggatt attgacagga tagatcacag gaacatcaat cttgaagttg
     1441 actttatgaa gggaaagaca gccacaaccg aaaaccttgc catttccatc tgggatgaac
     1501 tgaggccctt catagaacaa gagggtgcta agctccattg tgtgagaatc tgtgaaactg
     1561 aaaacaattc aatcgaatat tatggctgag aaaaatgaaa gcggaaaaat cagtgagctg
     1621 aatgtgcata atggctataa caggatagag tcatgggacg aggagagcat cagggaactt
     1681 tccggcatgt attacagggt attgaagctg cttggggaag atcctgaccg cgaaggactg
     1741 aggaaaactc ccgaaagggt ggcaaaagcc cttcatttcc tgatgatggg atactccatg
     1801 gatccctgtg agataatcaa ttccgccaag ttcagggaag attacaagca aatggttata
     1861 gtaaaaaata ttgacattta ttccatgtgt gagcatcaca tgcttccctt cttcggacag
     1921 gcgcatgttg cctatattcc cgacggctat atcaccggac tgagcaagat agcacgggtg
     1981 gtggaggcat acagccggcg cctgcaggta caggaaagac tgacggtaca gatacgcaac
     2041 actctgcagg actgcctcag gccgctcgga gtggcagtgg tcatcgaggc acagcacatg
     2101 tgcatgcaga tcagaggtgt tcagaaacag aattccgtca ccaccacatc tgcctttacg
     2161 ggaatctttc tttcaaatca aaacacacgt gaggagttta ttcacctcat tggttccgga
     2221 cttcactgaa acgaaacatg aaagcatatg tatttcccgg acagggagcc cagtttccgg
     2281 gtatgggaaa ggatctgtat gaaggatccg ccctggcaaa agaaatgttt gaaaaggcag
     2341 acaggctcct ggggtt
//
LOCUS       DAOSPX010000427         2320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_42142_length_2320_cov_3.933333, whole genome shotgun sequence.
ACCESSION   DAOSPX010000427 DAOSPX010000000
VERSION     DAOSPX010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42142_length_2320_cov_3.933333"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLZ75_14045"
     CDS             complement(<1..570)
                     /locus_tag="PLZ75_14045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOS73563.1"
                     /translation="MKYKWLDWVIQIQSIAQAGLAFAENQYDADRYRMLRDLSVEIMH
                     HYTEVSHEKLRDLFAGETGYQTPKVDVRASVISDDRILLVREKIDGKWALPGGWADVN
                     SSVSESVERECLEEAGVSVIPERIIALQLAGRHNDHPYPYTIYKVFVECKMVSGSFRD
                     NTETFAAGFFSRNDLPGLSTERTTRKQIDM"
     gene            651..1640
                     /locus_tag="PLZ75_14050"
     CDS             651..1640
                     /locus_tag="PLZ75_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOS73564.1"
                     /translation="MTFLYIIIGLVLIFDFINGFHDSANSIATIVSTKVLSPFNAVLM
                     AALFNFIAFTVFPLKVAATMGKGIIDPGAVTLTVIAGALVAAITWNLLTWWWGLPSSS
                     SHTLVGGLVGASIAGSGPGSVISGGVLKIAAFIVIAPLLGMIISFVISVIVMNLIRNF
                     SPSVIDKHFKRLQILSAAAFSLGHGGNDAQKSMGIIWIALITMGLSSIEDSRPDFWVI
                     ISCQAAIALGTLFGGWRIVKTMGQKITKLRSFEGFCAETAGALTLFGATHFGIPVSTT
                     HTITGSIIGVGAIKGLSAVKWGVTGKIFLAWIFTIPVSALIGALMYYFFEAIF"
     gene            complement(1655..>2320)
                     /locus_tag="PLZ75_14055"
     CDS             complement(1655..>2320)
                     /locus_tag="PLZ75_14055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS73565.1"
                     /translation="TLLPPEEDYLLADPFPAGSSAIPDIENNKDLKNAGLTRLMADYA
                     IKAAKFSYLPDIGLLGGYTYQEGTDIYPRNNAFIGASVQWNLQDVLTNRTVQRQRSLS
                     RLQAEENLINTREQVNRDIAAAMRRIRQAEELIKVAAKVCDYRSADLRIHTDKRRSGL
                     NLEADLLSARAAMARAEADLFAARMNHRIALTELEILTGNYREQAAHLPAGHSRMAPA
                     VQP"
BASE COUNT          528 a          584 c          556 g          652 t
ORIGIN      
        1 catgtcaatt tgtttcctgg ttgtccgttc cgttgaaagc cccggaaggt cgttccggga
       61 aaagaatcct gcggcaaatg tttctgtgtt gtccctgaag ctgccgctca ccattttaca
      121 ttcaacaaaa actttataaa ttgtataggg gtagggatgg tcattatgcc tgccggcaag
      181 ctgaagtgcg attatccttt caggtatgac cgacacgccc gcttcttcaa ggcattccct
      241 ttcaacggat tcactcaccg aggaattgac atctgcccag cctccgggaa gggcccattt
      301 cccgtcgatt ttttccctca ccagaaggat cctgtcatcg ctgatcacgg acgcccttac
      361 atcaaccttc ggagtctgat agccggtctc tcccgcaaaa agatccctca gcttctcgtg
      421 gctcacctca gtgtaatgat gcattatctc aaccgaaaga tccctcagca tccggtacct
      481 gtcggcatca tactgatttt ccgcaaaagc cagacctgcc tgagctattg actggatctg
      541 gatgacccag tcaagccatt tatatttcat aaaagaggaa atatattttg agtaatgaat
      601 tatttcgtaa aattgcaaca aaaatcaaac aaatgctaat cccgtcctga atgacatttc
      661 tatatataat catagggctg gttctgatat ttgatttcat taacggcttt catgattctg
      721 ccaattcgat agccactatc gtttcaacca aggttctgtc accattcaat gccgttctga
      781 tggcggcttt attcaatttt atagcattca ccgtattccc gctgaaggtt gcagccacca
      841 tgggtaaggg aattattgat ccgggagctg tcacactgac agttatagca ggtgcgctgg
      901 ttgcagccat cacctggaat ctgctcacct ggtggtgggg acttccctca agctcgtccc
      961 atacacttgt gggcggactg gtgggtgctt caattgccgg ttcaggaccg ggttcggtca
     1021 tttccggggg agtgctcaaa atagctgcat tcatcgtgat agctccgctg ctgggtatga
     1081 ttatatcatt tgtcatatcc gtaattgtaa tgaacctgat cagaaatttt tccccttcgg
     1141 ttattgacaa acacttcaaa aggcttcaga ttttgtcggc tgccgcattc agtctgggac
     1201 atggcgggaa cgatgcacag aaatcaatgg gtattatctg gatagctttg ataaccatgg
     1261 ggttatcttc tattgaagac agcaggccgg atttctgggt aattatttcg tgtcaggcag
     1321 ccatagctct cggaaccctt ttcggaggct ggagaatagt gaagaccatg ggtcagaaga
     1381 ttaccaagct cagatccttt gaaggctttt gtgcagaaac agccggggcc ctcactctgt
     1441 ttggagcaac ccatttcggc ataccggtca gcacaaccca caccatcacc ggatccatta
     1501 tcggtgtcgg tgcgatcaag ggtctgtcgg ccgttaaatg gggtgtcacc gggaagatat
     1561 ttcttgcctg gatatttacc atacccgtat cagcccttat cggtgccctg atgtattatt
     1621 tcttcgaggc aatattctga atatacagtg ggattcaggg ctgaacggca ggtgccattc
     1681 tgctgtggcc tgcaggcaga tgagcagcct gctcccggta attcccggtc agtatttcca
     1741 gttcagtcag ggcgatccgg tgattcatgc gtgccgcaaa cagatctgct tccgcccttg
     1801 ccattgctgc cctggcagat aaaagatcgg cctccaggtt caggcccgac ctgcgtttat
     1861 cagtatgtat cctgagatct gcgctgcggt aatcacacac ttttgcggca acctttatca
     1921 gttcctctgc ctgcctgatc ctgcgcattg ccgcggcaat atccctgttc acctgctccc
     1981 gtgtattgat caggttttcc tcagcctgca gcctgctgag cgacctctgt ctctgaacag
     2041 tcctgttggt cagtacgtcc tgaaggttcc actggactga ggccccgata aaagcattgt
     2101 ttctcgggta aatatcagtt ccttcctgat aggtgtagcc gcccaggagg cctatgtcgg
     2161 ggagatagct gaattttgcg gctttaatgg cataatcggc cattaaacgg gtcagtccgg
     2221 catttttcag atccttgttg ttttcaatat cagggattgc agaactgccg gccgggaagg
     2281 gatccgccag taaataatct tcctcggggg ggagaagtgt
//
LOCUS       DAOSPX010000428         2319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_42168_length_2319_cov_4.362633, whole genome shotgun sequence.
ACCESSION   DAOSPX010000428 DAOSPX010000000
VERSION     DAOSPX010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42168_length_2319_cov_4.362633"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="PLZ75_14060"
     CDS             <1..1377
                     /locus_tag="PLZ75_14060"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOS73566.1"
                     /translation="AESRELEAYVDSEADAPLLAEMPSPSPVVRRTENKNFRFTELSF
                     GNGVRMILMPTDFKNDEILLSAYSPGGISLYPDNEIMSATLAATVVTQSGLGDYDLTG
                     LQKLLSGNTAKLTPYISELLEGFSGSCSPKDLETLLQLNYLYFTGVRRDDDAYSSYMS
                     RMKNMIGPMRAIPRVKFADTLSKVITRNSPRVINLPTDAQLDQADLGRIMSIFRERFA
                     SAGDFTYIMVGNFDVNEVLPLLEKYIGGLPSVNGKEKWRDMTPGFPEGLVEIEVAENS
                     EPQSLVAMVWKGDFKWKPVERMGFSMLMEILSIKCRESMREDQGGVYGVSVSGTVSKN
                     PEPAYTITATWGCDPGKIDSLTATVISEMNSIIRKGPEQTDLNKVKETMIRQRETGMK
                     ENSFWISYLQSHFLNGNRILSFNEYRDAVNSVDAKTIKKIAGRYLDTKHYVKAALTPA
                     VKDTEPDS"
     gene            complement(1419..2033)
                     /locus_tag="PLZ75_14065"
     CDS             complement(1419..2033)
                     /locus_tag="PLZ75_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOS73567.1"
                     /translation="MHIAIAGNIGSGKTTLAGLLAKHYGWTPHYENADDNPYLNDFYE
                     DMLRWSFNLQIYFLNSRFSQIIQIKKSDKTIVQDRTIYEDAQIFAPNLHSMGLMSTRD
                     FENYSSLFRMMSSLIEPPDLLLYLRASVPTLVSQIQKRGREYESSIRIDYLKRLNERY
                     EAWVETYDLGKMLIIEADHYEFPTNRDHLSEVIDKINAELHGLF"
     gene            complement(2076..>2319)
                     /locus_tag="PLZ75_14070"
     CDS             complement(2076..>2319)
                     /locus_tag="PLZ75_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOS73568.1"
                     /translation="KEPADISLFATILDETLKEVNSDYEAKRYKDINLVMPVVRCVPA
                     GTFHKWMKSKNKLGGQNKVPRLSNGREYIEELYKFL"
BASE COUNT          649 a          485 c          576 g          609 t
ORIGIN      
        1 gcagaatcgc gtgagcttga ggcttatgtt gacagcgagg ctgatgctcc gcttctcgct
       61 gaaatgcctt ctccctctcc cgtagtaaga aggactgaaa ataaaaactt cagattcact
      121 gaactgagtt tcggaaatgg agtcaggatg attctgatgc caacagattt caaaaatgat
      181 gagatactcc tttctgccta cagtcccggc ggcatatccc tctatcccga taatgaaata
      241 atgtcggcaa cgcttgccgc gactgtggtg acgcaaagcg ggcttggcga ctatgatctg
      301 accggtttgc agaaattgtt gtcggggaac acggcgaaac tcacacctta tatcagcgaa
      361 ctgcttgaag gattcagcgg cagctgctca ccgaaggacc tggaaacgct tttgcagctg
      421 aactatcttt atttcaccgg ggtacgcagg gatgatgatg cttacagttc atacatgtcg
      481 agaatgaaga atatgatcgg gcccatgagg gcaataccga gagtgaagtt tgccgatacc
      541 ctctcaaagg ttattaccag gaattcaccc agggttatca atctacctac cgatgcacag
      601 ctcgatcagg cagatctcgg caggataatg agtattttcc gggaacgttt cgccagtgcg
      661 ggagatttca cctatataat ggtcggcaat tttgatgtga atgaagtgtt gccgctgctt
      721 gaaaagtata taggaggatt gccttcggta aacggaaagg aaaaatggag ggatatgacg
      781 cccggatttc ctgaagggct ggttgagatt gaagtagctg agaactcaga acctcagagc
      841 ctcgtagcaa tggtctggaa aggggatttt aagtggaagc ctgttgaaag gatgggtttt
      901 tcgatgctga tggagatact ttcgataaaa tgcagggaat cgatgcgcga agaccaggga
      961 ggtgtttacg gagtgtctgt cagcggtacg gtttcaaaga acccggaacc tgcatataca
     1021 ataaccgcaa catggggatg tgatcccgga aagattgaca gtcttacagc taccgttatc
     1081 agtgaaatga acagcataat acggaagggc cctgaacaaa cagatctgaa caaggtaaag
     1141 gaaacgatga tacgtcagcg ggagaccgga atgaaggaga acagtttctg gatatcctat
     1201 cttcagagtc attttcttaa cggtaacagg atcctgtcgt ttaatgaata cagggatgca
     1261 gtaaattcag tagatgcaaa gaccattaaa aaaatcgcag gaagatatct ggacacaaaa
     1321 cattacgtga aggcagccct taccccggca gtgaaggata cggaaccgga ttcttaatca
     1381 gattctgcat caggttcagg cccggaatag aaagaaattc agaacaagcc atgcagttcg
     1441 gcgttgatct tgtcgatcac ttcgctcaga tggtccctgt ttgtaggaaa ttcgtagtga
     1501 tcggcctcaa tgatgagcat tttcccgaga tcataggttt caacccatgc ctcatacctt
     1561 tcattgagcc ttttcaggta atcaattctg atggagcttt catattcacg gcctcttttc
     1621 tggatctggc tgaccagtgt gggaaccgat gccctcaggt aaagcagaag gtcgggaggc
     1681 tcgatcagag agctcatcat tctgaaaagg gaggaataat tttcaaagtc gcgtgttgac
     1741 atcagaccca ttgaatgaag attgggtgcg aaaatctgtg catcttcata aatagtcctg
     1801 tcctggacaa ttgtcttgtc agatttcttt atctgaataa tctggctgaa acgggaattg
     1861 agaaaataga tctgcagatt gaatgaccac ctgagcatat cctcatagaa atcgttgagg
     1921 tatggattat catcagcgtt ttcataatgc ggggtccatc cgtaatgctt tgcaagcaag
     1981 cccgccagag tggtctttcc tgatccgata ttacccgcga tggcaatgtg cataattgac
     2041 tttttttgac gtgtaaaaat attaaaaata tgtgattaca gaaatttata aagctcttca
     2101 atatactccc tgccgttaga caggcggggc accttattct gccccccgag cttgtttttc
     2161 gatttcatcc atttatggaa ggtgccggcc ggaacacatc ttaccaccgg cattacaaga
     2221 tttatatcct tgtagcgctt tgcctcataa tctgaattga cctccttgag ggtttcgtcg
     2281 agtatggttg caaaaaggct gatatccgcg ggttccttt
//
LOCUS       DAOSPX010000429         2302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_42585_length_2302_cov_3.828215, whole genome shotgun sequence.
ACCESSION   DAOSPX010000429 DAOSPX010000000
VERSION     DAOSPX010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42585_length_2302_cov_3.828215"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..989)
                     /locus_tag="PLZ75_14075"
     CDS             complement(258..989)
                     /locus_tag="PLZ75_14075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HOS73569.1"
                     /translation="MARKIIRIVSFLKGAILLMKPHILLGWLRKPFLKISNTLSLSRW
                     VSEQKGDFIINDFYTAKRDYSKRYKLYKAVSDEFRLKDKAITYMEFGVSGGSSFKWWL
                     NECRNEKSIFIGFDTFEGLPENWGTFKKGDMAAALPGFSDKRAVFVKGLFQDTLHKYF
                     RENKPDPEKRNIVHLDADLFSSTLFALSGISDFLKKGDILFFDEFNVPDHEFYAYRIF
                     SESWYIKTRLLGAVNNYLQTAFIID"
     gene            complement(1129..>2302)
                     /gene="hcp"
                     /locus_tag="PLZ75_14080"
                     /gene_synonym="priS"
     CDS             complement(1129..>2302)
                     /gene="hcp"
                     /locus_tag="PLZ75_14080"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOS73570.1"
                     /translation="EHAFNLGFEDNDIFDFMEQTLVMLRKPADLDSMLSWVMKTGEYG
                     VRVMALLDRANTSTYGNPEITKVNIGTRSRPGILVSGHDLRDLEQLLKQSEGQGVDIY
                     THSEMLPSHAYPALKKYSHLAGNYGNAWHRQLEEFETFNGPVLFTTNCLVPPRKTSTY
                     NDRIFTTGATGMPGWKSIDKKLPDGQKDFSEIIGLAKKCPPPSEIESGEITIGFAHNQ
                     VLSLADKILEAVNSGAIRKMVVMSGCDARQKSREYYTEFAKSLPADTVILTSGCAKYR
                     YNKLNLGDINGIPRVLDAGQCNDSYSWAYVALKLKEVLNLDDINKLPIVFNIAWYEQK
                     AVIVHLALLYLGIKNTHIGPTLPGFLTPNVLKIVQEKFGVRVNGTVEEDLKIFGLN"
BASE COUNT          609 a          493 c          496 g          704 t
ORIGIN      
        1 cttcctgaat cttcaagaag ccgtaaggcg gtaactgctc cattacttcc ttcactcttc
       61 atttgttcca gtctggatct cttgcttgaa accagggaaa tctcagacaa tgaaaaaaag
      121 ctgttaagca ggataagaat gaacaaaata agtatttcca tttgttccgg tgaaaaaaat
      181 gagtatttaa gataacggtt ctgtaatatg ccattcaaac tggcagttgc atcagccggt
      241 gaatgcatcc ggcagggtca gtcaattatg aaagctgtct gaagataatt gtttacagct
      301 ccaagcagcc tggttttaat gtaccatgac tcagaaaata tccggtaggc ataaaattca
      361 tgatccggta cattgaattc atcaaaaaaa agaatatccc cttttttcag gaaatccgat
      421 attcctgaaa gggcaaaaag tgttgaggag aacaggtcgg catcgagatg tacaatattt
      481 cttttctccg gatcaggttt gttttcacgg aaatacttat gcagggtgtc ctgaaacagg
      541 ccttttacaa atactgcgcg tttgtcactg aatccgggaa gagcggcagc catatccccc
      601 tttttgaaag ttccccagtt ttccggcaat ccctcgaaag tatcaaagcc gatgaaaatg
      661 cttttttcat tcctgcattc attcagccac catttgaatg aagagccgcc cgaaacaccg
      721 aattccatgt atgttatggc tttgtctttc agcctgaact catcagaaac ggctttatag
      781 agcttgtacc ttttcgagta atcccgtttt gccgtataga aatcatttat gatgaaatcc
      841 cctttttgtt cagataccca acgcgatagg ctcagtgtat ttgaaatctt gagaaatggt
      901 tttctgagcc agccaaggag aatgtgtggt ttcatcagca ggatggcacc tttcaggaat
      961 gatacaattc tgatgatttt tcttgccatg gggtaataat aaaaatggct gtttataaaa
     1021 tccctgcaat ccggattaaa ttatgcaaag atctttatta acagccagga aaaacagaag
     1081 gtttctgtta aattattgtg ctacaaatct tcaaataaac actatatttt agtttaatcc
     1141 aaaaattttc aaatcttctt ccacggtgcc attcacccgg actccgaatt tctcctgtac
     1201 aattttcagg acattgggtg tgaggaatcc gggcagggtg ggaccgatat gggtattttt
     1261 tattcccagg tagagcaatg caaggtgaac gataacggcc ttttgttcgt accatgcaat
     1321 attgaaaact atcggaagct tgtttatatc gtcaagattc agaacctcct tgagcttgag
     1381 tgccacatat gcccatgaat aggaatcatt gcactgtcct gcatcaagca ctctcggaat
     1441 tccgttgata tcacccaggt tcagtttgtt gtagcggtat tttgcacagc ctgatgtaag
     1501 gatgacagta tctgccggaa gactttttgc aaattccgta taatattccc ggcttttctg
     1561 gcgggcgtca cagcctgaca tcacgaccat ttttcttata gctcccgaat ttacagcttc
     1621 gaggatcttg tcagcaagag agagcacctg gttgtgtgca aaacctattg tgatttcgcc
     1681 gctttcaatt tccgagggtg gcggacattt ttttgcgagt ccgatgattt cggaaaaatc
     1741 cttttgcccg tccggcagtt ttttatcaat ggatttccac ccgggcattc ctgttgctcc
     1801 tgttgtaaat atcctgtcat tgtaagtact ggtttttctc ggcggaacaa gacagttggt
     1861 ggtgaaaagc acgggtccgt tgaaggtttc aaattcctcc agctggcggt gccatgcatt
     1921 gccgtaattg ccggcaaggt ggctgtattt ttttagtgcg ggatatgcat gggaaggaag
     1981 catttcggag tgagtataga tatccactcc ctgaccttct gactgtttca gcagttgttc
     2041 gagatccctg agatcatgtc cgctgacaag tattcccgga cggcttcggg ttcctatatt
     2101 tacctttgtt atttccggat tgccgtatgt tgaagtattt gccctgtcaa gaagagccat
     2161 taccctgact ccgtattcac ctgttttcat tacccagctg agcatggaat caagatcggc
     2221 cggctttctc agcattacca gggtctgttc catgaaatca aatatgtcgt tgtcttcaaa
     2281 gcccaggttg aaggcatgtt cg
//
LOCUS       DAOSPX010000430         2296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_42735_length_2296_cov_4.258813, whole genome shotgun sequence.
ACCESSION   DAOSPX010000430 DAOSPX010000000
VERSION     DAOSPX010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42735_length_2296_cov_4.258813"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PLZ75_14085"
     CDS             <1..841
                     /locus_tag="PLZ75_14085"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOS73571.1"
                     /translation="LGGYDIWETRGLGVWSKPRGIELSAEFASDDRKNWKISPKAFVR
                     KYDNRASEYMVDFQGKIHAGTRLSVEANIKGSREDNKTAWASNETFLYDDNKWKIGNK
                     STSPDKLDLTDFTAFDDGGMLGGILSGVKEYQSGMYYVPVFGTRDTRAIDFTVRGSLT
                     FTSKLSTQLYTQFFFAKGKYDNFSILTGPGTMPAFPVYPKNRDFNYKHLQSNFVARWE
                     YRPGSAIYFVWTHSRNRDEESNPLSPWNDLLYRQSLREQISGLFRTFPGNSFTVKIDY
                     AFH"
     gene            complement(863..1672)
                     /locus_tag="PLZ75_14090"
     CDS             complement(863..1672)
                     /locus_tag="PLZ75_14090"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS73572.1"
                     /translation="MKKVVLITGISSGFGKETARLLAEKGHTVYGTIRKDCEVPGSVK
                     ALRMDLTDEHSIKEAVEAVLKAENRIDVLVNNAGMHSGGAIETYPTELVRLHIETNIM
                     GTLSLIRKVLPRMRKQGGGMIINISSIGGLIGLPFQGYYSSVKFALEGISDALRMEVS
                     GFNIKVVVINPGDFNTNNSIARHEFLTPETEDDPYAGQFKKTLEIIERDENNGWGPEI
                     LAKKIVKIAECKNPRQRYIIASFDQKLAVFLKYILPGKLFRKILEMHYKIG"
     gene            1771..>2296
                     /locus_tag="PLZ75_14095"
     CDS             1771..>2296
                     /locus_tag="PLZ75_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73573.1"
                     /translation="MEKLYNYSLVLIFSLAVIVFLLLFFISAPYGKFRREGWGPVIRA
                     KWAWLIMEFISPVLMIFFFIISDRKSVPAFIFISLWLMHYVHRTFIYPFTQSGRNKPM
                     PLILVVFAIVFNSLNGLVNGYGIFHIYSYDLSWLLSWQFLAGAIVFISGFIINKTADE
                     KLRRLRNENPNEYVI"
BASE COUNT          609 a          493 c          505 g          689 t
ORIGIN      
        1 gctcggggga tatgatatct gggaaaccag gggtctggga gtatggtcaa agcccagggg
       61 tattgaatta tcggcagaat ttgccagtga tgacagaaaa aactggaaaa tcagtccgaa
      121 agcttttgta agaaaatatg acaacagggc ctcggaatat atggttgatt tccagggtaa
      181 gatacatgcc ggaacaagac tgtcagtgga agcaaacatc aagggcagcc gggaagataa
      241 taaaaccgca tgggcatcaa atgaaacatt tctgtatgat gataataagt ggaaaatagg
      301 caacaagtcg acatcaccgg acaagctgga tctgacagat tttaccgctt ttgatgatgg
      361 cgggatgctg ggtggcattc tttcaggcgt aaaggaatac cagtccggaa tgtattatgt
      421 gccggtcttc ggaacacgcg acacgcgggc aattgatttc accgtaaggg gatcgcttac
      481 ttttaccagc aagttgtcaa ctcagctcta tacacagttc ttctttgcca aagggaaata
      541 cgacaatttc agcatcctta cgggtcccgg aacaatgccg gcttttccag tgtatcctaa
      601 aaacagggat tttaattaca agcacctgca gtccaatttt gttgccagat gggaatacag
      661 gccgggctcg gcaatatatt ttgtatggac tcattcccgc aacagggacg aagagagcaa
      721 tccgctttct ccctggaatg atctgctata caggcagtca ctcagggaac agatttccgg
      781 gctcttcagg acattcccgg gaaacagttt cacggttaag attgattatg catttcactg
      841 aacaaatgca gcagcacccc tgtcatccga ttttataatg catttcaaga atcttccgga
      901 acaatttgcc ggggaggata tatttcagaa agacagctaa tttctgatcg aaggatgcaa
      961 ttatgtaacg ttgccgggga tttttgcatt ctgctatttt aacaattttt ttcgccagga
     1021 tttccggacc ccagccattg ttttcatctc tttcgattat ttcaagggtt tttttaaatt
     1081 gtccggcata agggtcatcc tcagtttccg gagtaaggaa ttcatgccgg gctattgaat
     1141 tattcgtatt gaagtcaccg ggatttatca ctaccacttt tatattgaat cctgacactt
     1201 ccattctgag ggcatcactt ataccctcga gggcaaactt cactgatgaa tagtatccct
     1261 ggaaagggag gcctatcagt ccgccgatgg aactgatatt tattatcata cctccaccct
     1321 gctttctcat ccggggaaga accttcctga tcagcgaaag cgtgcccatt atgttggttt
     1381 caatatgcag cctgacaagt tcggtgggat atgtttcaat ggcacctccg gaatgcattc
     1441 cggcattgtt gacaagaacg tcaattctgt tttctgcctt caggacagct tcaacagcct
     1501 cctttattga atgctcgtcc gtaagatcca ttctgagagc ttttacagag cctggcactt
     1561 cacaatcctt ccggattgtt ccgtaaacgg tatggccttt ttcagccagg agtctggctg
     1621 tctctttacc gaaacccgac gaaattccgg ttattaaaac aacttttttc atcatccggt
     1681 tgttctgttt ttaaacccct gaaaaaaatc aattccgata aatatataaa tttttacttt
     1741 tgccgcattc aaaccagatc tcaatgataa atggaaaagc tatataatta ttcccttgtt
     1801 ttgatttttt ccctggcagt aattgttttt cttttgctgt tttttatttc cgctccctac
     1861 gggaaattcc ggagagaagg ctgggggccg gtgatcaggg ccaaatgggc atggctgatc
     1921 atggaattca tttcacccgt cctgatgatt ttctttttca tcatttcgga caggaaatca
     1981 gtgccggctt ttattttcat ttccctgtgg ctgatgcatt atgttcacag aacatttatt
     2041 tatccgttca cccagtcagg cagaaacaaa ccgatgcctc tgattcttgt ggtttttgca
     2101 attgtattta acagtctcaa cggtctggtt aacggttatg gcattttcca tatctacagc
     2161 tatgatttgt cgtggttgct gtcgtggcag ttccttgccg gtgcaattgt atttatcagc
     2221 ggtttcataa ttaataaaac agctgatgag aagctgcgca gactcaggaa tgaaaatccg
     2281 aatgaatatg tgattc
//
LOCUS       DAOSPX010000431         2286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_42988_length_2286_cov_3.467055, whole genome shotgun sequence.
ACCESSION   DAOSPX010000431 DAOSPX010000000
VERSION     DAOSPX010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42988_length_2286_cov_3.467055"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLZ75_14100"
     CDS             complement(<1..1285)
                     /locus_tag="PLZ75_14100"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS73574.1"
                     /translation="MKIIRTTFMLMALLLAAAGSNACKKCPDCPDTGFPCIKIDPVSL
                     TFGNVEAGAEASQEIVVKNTGSGTLTILSVTSSDPAFTGSFAVSAIPPDQEAVVTVRF
                     SPALAKGYSGVLTINSDAVCSQARTVALTGIGRGQTTTPLFSSSLETASTGDPERPDG
                     WWQDFWGISGSNVTFTYLNEGHTGSRSVKVEVSGEPLHPHSGRFPQGDAKWLFEPAEL
                     TPGDYVYTNWYRSDVSTYVTLAITNKEGGTEYYDLPEAPASTGWTKYEAVFDVPANGV
                     AATVLHMLKSNGYLITDDHQIAPYYYKGFNKGLLTITFDDGWNINTETALPVMNRYGF
                     KSVQYYRTDAIQDYAGFQYPEPKSLIQIFKDAGHEFGSHSVNHSDLTLEPDADLTKEL
                     ADSKTFLENYLDITIKHFATPFGLYDSRVTKEAMKH"
     gene            complement(2014..>2286)
                     /locus_tag="PLZ75_14105"
     CDS             complement(2014..>2286)
                     /locus_tag="PLZ75_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73575.1"
                     /translation="SIIWLFSGGILFFFYFYGFGAENEQVVNIEGIPCVEMKIAFHGK
                     KNTSILPFESKPEAGCAGATTGRAITISIKIFFIRMFKLILINSFV"
BASE COUNT          605 a          560 c          510 g          611 t
ORIGIN      
        1 aatgcttcat cgcctctttc gttacccgtg aatcataaag tccgaaagga gtggcaaaat
       61 gttttatagt gatatccaga tagttctcca gaaatgtctt tgaatcggca agttctttgg
      121 ttaagtccgc atccggctcc agggtgagat cactgtggtt gacagagtgg gaaccaaatt
      181 catgtcctgc atccttaaaa atctgtatca ggctcttcgg ctccggatac tggaacccag
      241 cataatcctg aatcgcatca gtcctgtaat actgaaccga tttgaatccg tacctgttca
      301 tcaccggcag ggccgtttca gtatttatgt tccagccatc gtcgaatgta atggtgagca
      361 gacctttgtt aaagcctttg tagtaatagg gtgctatctg atggtcgtcg gtaatcaggt
      421 agccgttgct ctttagcatg tgcaaaacag tggccgccac accgtttgca ggcacatcga
      481 atactgcttc gtactttgtc caccccgtgc ttgcaggtgc ctcaggaaga tcataatact
      541 ccgttccccc ctccttgttg gtaatcgcca gggtaacata tgtgctgaca tccgaacggt
      601 accagtttgt gtagacatag tcgcccggtg tgagctctgc cggctcgaac agccatttgg
      661 catcaccttg cgggaagcgt ccggaatgag gatgcagagg ctcgcccgat acttccactt
      721 tcacgctgcg gctgccggtg tgtccctcat tcaaataagt aaacgtgaca ttggaaccgg
      781 aaattcccca gaaatcctgc caccagccat ccgggcgctc cggatcaccc gtactggcgg
      841 tctccagcga ggaactgaac agcggggttg tggtctgtcc cctccctatg cccgtcaggg
      901 caacagtcct ggcctggctg catacggcat cgctattgat tgtgagaacc ccgctgtatc
      961 ccttggcaag cgcaggggaa aaccttacag tcactacagc ctcctggtca ggcggaatag
     1021 ctgaaacggc aaatgaaccc gtgaaggcag ggtcggaact tgttaccgac aagatagtca
     1081 atgtaccgct gccggtattt ttcaccacaa tctcctgtga tgcttctgcc cccgcctcca
     1141 cattgccgaa tgtcagagag acaggatcaa tcttaataca gggaaagccc gtatcagggc
     1201 agtcaggaca ttttttgcat gcattgctgc cggctgctgc cagaagcagc gccattagca
     1261 tgaatgttgt tctgataatt ttcatttgtt tttctttata tcattttccg gattttctgc
     1321 cttcagtaat aatttgtatg ctgccggcag tccaaataaa cggaaaatcc atgccatgcc
     1381 tagccatacc atgggcattg tgcctttgtc accttctcct gaaaattacc tgctttttct
     1441 tctttacgac tttcaaccca tgctatgtta tgttaaacgg tacaaaccgc ttttatgttt
     1501 tcaaaatgca acatattttc ttttcagcac acggcaaaga atgtcaatcc aacagactgt
     1561 ccactggcag actggtagtc tgcaggggat caggtggctt aatacaactg tatgcaccgc
     1621 ccttttactg agcttattgt accggtaata taatgcagat accggaggca ggaaggaaga
     1681 aacagcagaa ttcaggaaga agtttgctag tcgggcgtta agttcacaga cacagaactc
     1741 tataataaac ctgattgacg ggaaagcaga tatcacaatc gctgccagaa gcctgtcacc
     1801 cgatgagaaa gaatatgccc cgggaaaagg gtttagggtg atcgaaacaa cgaagtggga
     1861 tgagcccggg gtgattattg cacctggcca tcccggtact ccacgctgca agtccctctg
     1921 taacaggagc agcggcatat gcaacaatga aaaaaccagc cccctgataa cattttaact
     1981 ttttgattgt tgaattctct aattggacaa tcgttaaaca aaactattta ttaagattaa
     2041 tttaaacatc cttatgaaaa agatttttat gcttatggtg atagccctgc cagtagttgc
     2101 gcctgcgcaa cctgcctcag gttttgattc gaaaggcaaa attgaggtat ttttctttcc
     2161 atgaaaagca attttcattt caacacatgg aattccttct atgttgacaa cctgctcatt
     2221 ttcagcacca aagccataga aataaaagaa gaataatatc cctccagaaa aaagccaaat
     2281 aatact
//
LOCUS       DAOSPX010000432         2219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_44782_length_2219_cov_4.989834, whole genome shotgun sequence.
ACCESSION   DAOSPX010000432 DAOSPX010000000
VERSION     DAOSPX010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44782_length_2219_cov_4.989834"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PLZ75_14110"
     CDS             <1..1314
                     /locus_tag="PLZ75_14110"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOS73576.1"
                     /translation="QGSQTGTVVYQETFNTLTNGNGLATIEIGSGTPVTGNFPSINWA
                     SGTYYLRTETDPAGGTNYTISGTNQLISVPYALHAKNAASYSETDPVFGAAPAKNITS
                     GNITNWNTAYSWGNHAGLYRPAGWVPAWNDVGGKPVFATVATSGNYNDLINKPTIVNS
                     PWTTTGFTIHYTKGNVGIGMSDPLYYPLTIYNPYSSYITFYNSTSGTGLNDGFQIGTT
                     HQGSPVWIWNCENSNMHFGTNNETRMIIHGDGHVEMTRSLDLNTDSNFGALHVQGKQA
                     LWWDGTYFSWGYDAVWNYFARKVSIGRAADPGSNMLYVQGSAFATGTWNSSDASFKKN
                     ISTIGNSLDRLMKVRGTRFEFRTDEFKDYHFTKGPQYGFIAQELEKEFPEVVRTEGDG
                     YKSVNYNGMIPVLLEAIKEQQKQIENQQKQIDELKMAFELMRKTE"
     gene            complement(1495..>2219)
                     /locus_tag="PLZ75_14115"
     CDS             complement(1495..>2219)
                     /locus_tag="PLZ75_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73577.1"
                     /translation="TVFRGQGKLKLVTPCQNDDDLIDEYLVYKLYNRVTDLSLKARLV
                     KVEYYDTAGDSSIYERYSFFLENKKHTAGRNGLVQKDYFLTPFSLNRDNYMKLAIFQY
                     MIGNKDWFVTSRHNIIIMQTDDGDQNPYAVPYDFDFSGFVNASYTYLPQDTKSALTSR
                     RIFRGLCYTEDEFRQVFKFYRALRPEFRAVIKKQEHLSQRSKRYVLNYIGQFYNIIGS
                     RDLFEKEFLAHCETKKNYNIPE"
BASE COUNT          668 a          502 c          480 g          569 t
ORIGIN      
        1 cagggctctc agacgggaac tgtggtttat caggaaacct ttaacacact tacaaacgga
       61 aatgggcttg ccacaattga aatcggaagc ggaactcctg ttacaggcaa cttcccatca
      121 ataaactggg catcaggtac ctattatctc cggaccgaaa cagatcctgc cggagggacc
      181 aattatacta tttcaggaac aaaccagctg ataagtgttc cttatgcttt gcacgctaaa
      241 aatgcagcca gttacagcga aactgacccg gtttttggtg ctgctccggc aaaaaacatt
      301 acaagcggaa atattaccaa ctggaatact gcttacagct ggggtaatca tgccggatta
      361 taccggcctg caggctgggt gccggcatgg aatgatgtag gcggcaaacc ggtatttgca
      421 accgtcgcaa caagcggcaa ttataatgac ctgatcaata aacccactat tgtaaacagt
      481 ccatggacta caacaggatt tactattcat tacaccaaag gaaatgtcgg gatcggcatg
      541 agtgacccac tttattatcc actaacaata tacaatcctt actcttcgta cataacattt
      601 tataatagta catccgggac cggccttaat gatggttttc aaattggtac aactcaccag
      661 gggagtccgg tgtggatatg gaactgtgaa aacagtaaca tgcatttcgg caccaataat
      721 gaaacccgta tgataattca cggtgacggc catgtggaga tgacgcgttc cctggatctg
      781 aataccgata gcaacttcgg ggctcttcac gtacaaggga aacaggctct gtggtgggat
      841 ggtacatatt tcagctgggg atatgatgct gtctggaatt attttgcccg caaagtatca
      901 atagggagag cagccgatcc cggcagcaat atgttatatg ttcagggatc cgctttcgcg
      961 acaggaacat ggaactcctc cgatgcatct ttcaaaaaga atatatcaac aatcggcaat
     1021 tcacttgaca ggctgatgaa ggtccggggg acccggttcg agttccgtac cgatgaattc
     1081 aaggattatc attttacaaa aggaccccag tacggattta ttgcccagga actggaaaag
     1141 gaattccccg aagtagtcag gaccgaagga gacggttaca aatcagtaaa ttacaacgga
     1201 atgataccgg tcctgcttga agcaataaaa gaacagcaga aacagattga gaatcagcag
     1261 aaacagattg atgaactgaa aatggctttc gaattaatga gaaaaacgga atgacatccg
     1321 tcacgcatat ggctgaatat gaatgcatcc gaagctccgc aaggattcag caaatacccg
     1381 acacgtagtc ccggggttaa ttcctcatac ccgcctgaag aatgaaccgg atagaattca
     1441 aaaggaaata cagctgactg tcttcaccga tgtccggcaa aaccggctgt aaagctattc
     1501 agggatgttg tagttctttt tcgtctcgca atgcgcaagg aattcctttt caaaaagatc
     1561 cctgctgcct attatattgt aaaactggcc aatgtaattc aggacatacc ttttacttcg
     1621 ctgagacaga tgttcctgct tttttatcac tgccctgaat tcaggccgca gtgccctgta
     1681 aaatttaaat acctgcctga attcatcctc ggtatagcat aagcccctga atatcctgcg
     1741 gctggtcagt gcactttttg tgtcttgcgg aagatatgta taggaagcat tgacaaatcc
     1801 cgaaaaatcg aaatcatacg gaactgcata agggttctga tctccgtcat ccgtttgcat
     1861 gatgataata ttatgccgtg atgttacaaa ccagtctttg ttacctatca tatactggaa
     1921 tattgcaagc ttcatataat tatccctgtt aagcgaaaac ggggtaagga aataatcctt
     1981 ttgtaccagt ccgttcctgc cggcagtatg tttcttgttt tccaggaaga aactataacg
     2041 ctcataaata ctgctgtccc cggctgtatc ataatactct accttaacaa gcctcgcctt
     2101 aagactcaga tcagttaccc ggttataaag cttgtaaacc agatattcat caataagatc
     2161 atcatcattc tggcagggag tgacaagctt aagcttcccc tgccccctga ataccgtat
//
LOCUS       DAOSPX010000433         2198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_45334_length_2198_cov_3.737284, whole genome shotgun sequence.
ACCESSION   DAOSPX010000433 DAOSPX010000000
VERSION     DAOSPX010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45334_length_2198_cov_3.737284"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1930
                     /locus_tag="PLZ75_14120"
     CDS             <1..1930
                     /locus_tag="PLZ75_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS73578.1"
                     /translation="LNKKVSGGQRKRLNIGLELMREPVVLFVDEPTSGLSSFDSGKVM
                     NLLRNQALNGRMVFAIIHQPSSDIIKMLDRLWILDKGGYMIYDGDPVEAIVYFKTETS
                     QANAAESECPSCGNIETDNILDIVEVKMIDEDGRPEKTRQVSPLEWYGKYRKKMMPFP
                     EKVTEKTEITHGSFRVPDRAAQVRTFIKRNLITKFADRQYMTINLLEAPLLAFILGYI
                     SKYSRNGDYLFSANVNYPVFLFMSVIVAIFMGLTVSAEEIFRDRKILEREKFLNLSRL
                     SYLFSKINFLFVLSAIQTLSFVLFASAILEIRGMMFRHWLILFSTACFGNLLGLNISA
                     GMRTAVSIYILIPLLLVPQLLLGGAMIQFDELHKTFSRKKYVPVIGDLMATRWAYEAM
                     TVEQFRNNRYERNFFDYDMAVSQNSWKASFLIPGLKVKVQECIRAGRDPEYREYIENN
                     FRKINYHVGEMSEITGIKPGSWFERVNYDEFDGITGDELRYYLDSLGMTFRQRERRIS
                     LQLDSLKRSIENRMGEKQFVRLLEENHNERLAELVLNRRSTLKIIEKDDRFIQKADPV
                     FMPPESKYGRAHFYAPFKQIGKLRTGTLVFNTAVIWMMTAILFCTLYYNLLKAFIIWL
                     EKLKLPFWRKFGRSLLQM"
     gene            1955..>2198
                     /locus_tag="PLZ75_14125"
     CDS             1955..>2198
                     /locus_tag="PLZ75_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOS73579.1"
                     /translation="MNLVRPKKNLGQHFLKDREIARKVASSLGAKECDSVLEIGPGTG
                     VLTEYLVKRNFPDFRVIEIDNESVHYLREKFPAMEYI"
BASE COUNT          638 a          457 c          560 g          543 t
ORIGIN      
        1 actcaataaa aaagtcagcg gagggcagcg aaaaagactc aatatcggtc ttgaactgat
       61 gcgtgagccc gtagtccttt ttgtggatga acccacttcg ggactgtctt cctttgattc
      121 cgggaaggtt atgaaccttc tgaggaatca ggccctgaac ggcaggatgg tttttgcaat
      181 aatccatcag ccttcatcgg atattatcaa gatgctcgac aggctgtgga tacttgacaa
      241 gggcggctat atgatctatg acggcgaccc tgttgaggcc attgtctatt tcaagacgga
      301 aacatcacag gccaatgccg cagaaagtga atgtccgagc tgcgggaata tcgagactga
      361 caatatactt gatattgttg aggtcaaaat gatcgatgag gacgggagac ctgagaaaac
      421 caggcaggta agtccgctgg agtggtacgg gaaataccgt aagaaaatga tgcccttccc
      481 ggagaaggta acggaaaaga cagagattac tcacggcagc ttcagggtgc cggacagggc
      541 agcacaggtg aggacattca taaaaaggaa tctcatcacc aaatttgccg acaggcagta
      601 tatgacgata aatcttcttg aagccccgct gctggccttt atcctgggat atatttccaa
      661 gtacagccgg aacggcgatt atctgttttc tgccaatgta aactatccgg tcttcctgtt
      721 catgtcagtc attgttgcaa ttttcatggg actgacggta agtgccgagg aaatattccg
      781 tgacagaaaa attctggaaa gggagaaatt cctgaatctg agcagactga gttatctgtt
      841 ttcgaaaata aatttccttt ttgtgctttc ggccatacag actctttcct ttgtcctatt
      901 tgcaagtgca atacttgaaa tcaggggaat gatgttcagg cactggctca tcctgttttc
      961 cacggcatgc ttcggcaacc tgctgggcct gaacatatcc gcgggaatga gaacggctgt
     1021 cagcatttat attcttattc ctctgctgct ggttccccag ctgcttctcg gaggtgcaat
     1081 gatccagttt gatgagctgc acaagacatt cagcagaaaa aaatatgttc ccgtgatagg
     1141 tgatctcatg gcaaccagat gggcatatga ggcaatgact gttgaacagt tcaggaacaa
     1201 caggtacgag aggaatttct ttgactatga catggccgta agccagaaca gctggaaggc
     1261 ctccttcctc attccggggc tgaaggttaa ggttcaggaa tgtatcaggg caggcaggga
     1321 tcctgaatac agggaatata ttgaaaataa tttccgcaag ataaattacc atgtagggga
     1381 aatgtcggaa ataaccggca taaaaccggg cagctggttt gaaagggtga attatgatga
     1441 gtttgacggg ataacaggag atgaactgcg ttactatctc gacagtctgg gaatgacttt
     1501 caggcaaagg gaaagaagaa tttcccttca gcttgattca ctgaagagga gcattgaaaa
     1561 cagaatgggt gaaaaacagt ttgtcaggct tctcgaggaa aaccataatg aaaggcttgc
     1621 cgaacttgta ctgaacagga gaagtaccct gaaaataatt gagaaggacg acaggttcat
     1681 ccagaaggcc gatcccgtat tcatgcctcc ggaatcaaaa tacggcagag cccatttcta
     1741 tgcgcctttc aaacagatcg ggaaactcag gacaggaacc cttgttttca atacggcggt
     1801 tatatggatg atgacggcga tcctgttctg cacactttat tacaacctgc ttaaggcctt
     1861 cataatctgg cttgaaaagc tcaagctgcc attctggaga aaattcgggc gcagcctgct
     1921 ccagatgtag ggcctgatgt gaatctgaaa gaaaatgaac ctggtccgtc caaagaaaaa
     1981 cctcgggcag cactttctca aagacaggga gattgcacgt aaagtggcat cctcacttgg
     2041 tgcaaaggaa tgtgattcag tccttgagat aggcccggga accggagttc tcacggaata
     2101 ccttgtgaag agaaatttcc ccgacttcag ggtgatagaa atagacaatg agtcggttca
     2161 ttatctcagg gagaagtttc cggctatgga atacatta
//
LOCUS       DAOSPX010000434         2194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_45471_length_2194_cov_4.526414, whole genome shotgun sequence.
ACCESSION   DAOSPX010000434 DAOSPX010000000
VERSION     DAOSPX010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45471_length_2194_cov_4.526414"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLZ75_14130"
     CDS             <1..439
                     /locus_tag="PLZ75_14130"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOS73580.1"
                     /translation="DFIRSRINQTAFLGSKLKEYGIPVVEPFGGHAIFIDVDRFLPLV
                     PKEEFPAQVLVCEIFIEGGVRGSEVGTLLADRNPLTRENRYPANEFVRLAIPRRVYTD
                     NHMSYVAAVVKNVFNRKGAINKGYRIIKEEPILRYFTLELEVA"
     gene            510..1385
                     /locus_tag="PLZ75_14135"
     CDS             510..1385
                     /locus_tag="PLZ75_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOS73581.1"
                     /translation="MIKSMTGYGKAVAEIPGKKVLIELKSLNSRQFDLNTRLPWLYRE
                     KESEIRNRISRLLDRGKIDLVISFDITDAESVPLINKAVVRSYYTQIREISGELGIEP
                     GMDLLSAVLKFPESLKTEKTELPVEEWNEVLEKLDESLQQLDTFRTEEGRSIESDLRL
                     CIGRIISLLDEIGRYEGNRIERIRERLHRLLEENVGGENIDGNRFEQEILFYLEKLDI
                     NEEKVRLRNHCEYFIEKIETPPPNGKILNFIAQEIGREINTIGSKANDSKIQKLVVMM
                     KDDLEKIKEQTLNIL"
     gene            1404..1982
                     /gene="gmk"
                     /locus_tag="PLZ75_14140"
     CDS             1404..1982
                     /gene="gmk"
                     /locus_tag="PLZ75_14140"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOS73582.1"
                     /translation="MNGRLIIISAPSGAGKSTLVNFLMDRPELDLEFSVSATTREPRG
                     SEKNGSHYHFITVREFRKRIRRGDFIEWEEVYRNQYYGTPREEIERIFRKGKNVLFDV
                     DVKGGINLKNIFGNKSLSVFIRPPSVEELEKRLTGRGTDSPEKIKMRLEKALEEMKLA
                     GLFDYTVVNDILEQAQKEIYTIIKNFIERQDE"
     gene            1975..>2194
                     /locus_tag="PLZ75_14145"
     CDS             1975..>2194
                     /locus_tag="PLZ75_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOS73583.1"
                     /translation="MSKIGLYFGSFNPIHIGHIVIAGYMTEFTPIEEIWFVVSPQNPL
                     KKKGSLLDNYQRLHMVNLGIGRNYRMKAS"
BASE COUNT          736 a          425 c          527 g          506 t
ORIGIN      
        1 agatttcatc agatcaagaa taaaccagac ggcatttctt ggttcaaagc ttaaggaata
       61 cggaatcccg gttgtggaac ctttcggcgg acatgcaatt ttcattgatg tggacagatt
      121 ccttccgctt gtgccaaaag aagaatttcc cgcacaggtg cttgtctgtg aaatatttat
      181 tgaaggcggt gtaagaggaa gcgaagtggg caccctgctg gctgaccgga atccgctaac
      241 acgtgaaaac cgttatcctg caaatgaatt tgtcaggctg gctatccccc ggagggttta
      301 tacagacaac catatgtcat atgtagcggc tgtcgtgaaa aatgtattca acagaaaagg
      361 agcaataaac aagggataca ggatcataaa ggaagagccc atcctccgct atttcaccct
      421 tgagctggaa gtggcctgat tttaacggaa tgcagccagt ctgaatcctt ttgcatattt
      481 ttgccattta aaaccaatcc ctgccagaga tgataaaatc catgacaggc tacgggaaag
      541 ccgtagcgga aattcccgga aagaaggtat taatagaact gaaatcactt aattccaggc
      601 aattcgatct gaatacacga ttgccatggc tgtacaggga aaaagaaagt gaaatacgca
      661 accgtataag caggctgctt gacaggggga aaattgatct ggtaatatct ttcgatataa
      721 cggatgcaga atcagttccg ctgatcaaca aagctgtggt aagatcctat tacacccaga
      781 tcagggaaat atccggagaa ctcggcattg aaccgggtat ggacctgctg tcggctgtac
      841 tgaaattccc ggaatccctg aaaacagaaa aaacggaact gcccgtagaa gagtggaacg
      901 aggtactgga aaaacttgat gaatcgcttc agcaactcga tactttcagg acggaagaag
      961 gcagatcaat tgaatctgat ctcagactgt gtataggcag gataatctcc ctcctcgacg
     1021 agataggaag atatgaggga aacagaattg agagaataag ggaaaggctg cacaggcttc
     1081 ttgaggaaaa tgtgggcgga gaaaatatcg acgggaacag gtttgaacag gagatacttt
     1141 tttatctgga aaaactcgac atcaatgaag agaaggtaag actgaggaat cattgcgaat
     1201 attttattga aaagattgaa acaccgccgc ccaacggaaa aatactgaat ttcatagcac
     1261 aggaaatagg ccgggaaatc aataccatcg gctccaaggc aaatgattca aaaatccaga
     1321 aactggtggt gatgatgaag gacgaccttg aaaaaataaa ggaacagaca cttaacatac
     1381 tctgagattc cgtggacagt tcaatgaacg gcagactgat aatcatttca gccccctcgg
     1441 gcgcaggaaa aagcactctt gtaaactttc ttatggaccg cccggaactt gacctggaat
     1501 tttctgtttc ggcaactacc agggaaccaa gaggcagtga aaaaaacggg agtcattacc
     1561 atttcatcac cgtcagggaa ttcaggaaaa gaatcaggag gggagatttt attgaatggg
     1621 aggaagtata caggaaccag tactatggaa cacccaggga agagattgaa aggattttca
     1681 gaaaaggaaa aaatgtcctc ttcgatgttg atgtaaaagg aggaataaat cttaaaaaca
     1741 tttttggcaa taagtcattg tcggttttta ttaggcctcc atcggtggag gagctggaaa
     1801 aaaggcttac cggcagggga accgattccc cggaaaaaat aaaaatgagg cttgaaaaag
     1861 ctcttgaaga aatgaagctt gccggactgt ttgattacac tgtggtcaac gacatactgg
     1921 aacaggctca gaaggaaatc tataccatta taaaaaattt cattgaaaga caggatgagt
     1981 aaaatcggac tctatttcgg atcattcaac cccatccata tcggtcatat cgtaatcgcg
     2041 ggctatatga ccgaatttac tcccattgaa gaaatctggt ttgttgtcag tccccagaat
     2101 cccctgaaaa aaaaaggaag ccttcttgat aattaccagc ggcttcacat ggtaaacctg
     2161 ggtattggca ggaactacag gatgaaagca tcgg
//
LOCUS       DAOSPX010000435         2143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_46980_length_2143_cov_3.855843, whole genome shotgun sequence.
ACCESSION   DAOSPX010000435 DAOSPX010000000
VERSION     DAOSPX010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46980_length_2143_cov_3.855843"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1021
                     /locus_tag="PLZ75_14150"
     CDS             89..1021
                     /locus_tag="PLZ75_14150"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73584.1"
                     /translation="MNKNSFLVFFLFILSTLVYGQTIEAPPEYIKALTSDWKGERFPD
                     GRPKVPDELLERLANISIEEAWGFLRNRGYSNQFENDWYIVNPDEPMTGRVVTAQYIP
                     LRPDLEKYVRDRGKAEGRTQQGGTNSWPIDLLTEGDVYVADSYGKIIDGTLIGDNLGN
                     SIYSKSGKGVVFNGSVRDAEGLSEIEGFNGWIKGHDPSAIAQMSLLSINYPVRIGRAV
                     VLPGDAVLAKKYGVIFIPAHLLYECVINAEVTNLRDKFGHQRLREGRYKPGEIDTRWS
                     EEIKKDFLEWLRNYPEKLPMSKTELDNYFKERNW"
     gene            1086..>2143
                     /locus_tag="PLZ75_14155"
     CDS             1086..>2143
                     /locus_tag="PLZ75_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOS73585.1"
                     /translation="MKNLIKKLQEENRKLETAEADAIQAEINNPATGSGRRDFLKKTA
                     LGGLSLGGLMALSVEDTIAFTTQKVRRASSPSDLKITDMRYATIMNRHIIRIDTNQGI
                     YGLGEVRDAADVRYALFLKSRILGLNPCNVEMIFKIIRQFGYHGRQGGGVCAVEMALW
                     DLTGKAYNVPAWQLLGGRYREKVRLYADTPDAGDPVKFAEVMKKRVQDQGFTWLKMDL
                     GIHVVTKIPDALVNSKFWDGAVGQYDLRDYMGYGNTLHPFTQVQITERGLAGLAEYVE
                     KVRDEVGYEIPLCADHFGHFDLNNSIRFGRAMEKYRLAWLEDMVPWFDTVNNKKLSDA
                     LETPIATGEDIYCLRDGF"
BASE COUNT          647 a          459 c          526 g          511 t
ORIGIN      
        1 ttagcccggc ctgaattact ggatcctgaa ttgattttct ttgtgaaatg gttttatttt
       61 gaattttaac catataaaac tgtgcaacat gaataaaaac tcatttcttg tctttttcct
      121 tttcattttg tcaacactgg tatacggaca gactattgaa gcccctcccg aatacatcaa
      181 agcccttacg tctgactgga aaggggaaag attccctgac ggccggccca aagtaccgga
      241 tgaactgctg gaaaggcttg caaatatttc cattgaagaa gcctggggat tcctccggaa
      301 cagaggttat tccaatcagt tcgaaaatga ctggtatatt gtaaaccccg acgaacccat
      361 gacaggtcgc gtggtaacag cacaatacat acctctcaga ccggacctgg aaaaatatgt
      421 acgggaccgg ggaaaagcag agggcaggac acaacagggc ggcacaaact catggcccat
      481 cgatctgctt acagagggcg atgtttatgt tgcagacagt tacggaaaaa ttatcgatgg
      541 cacccttatc ggggataatc tcggaaactc catctattcg aaatcaggaa aaggtgttgt
      601 tttcaacgga tctgtcagag atgcagaagg tctgagcgaa atcgaaggat tcaacggctg
      661 gataaagggt catgatccct cagctattgc acagatgagc ctgctctcaa tcaattatcc
      721 tgtcaggata ggaagagctg tcgttctacc cggggatgcc gtactggcaa agaaatacgg
      781 tgttatcttt ataccggcac atcttctgta tgaatgtgtc attaacgcgg aggttacaaa
      841 cttacgtgac aagtttggtc atcagcgtct cagagaagga cgctacaaac ccggagagat
      901 agacacccgg tggtctgaag aaataaagaa ggatttcctt gaatggcttc gcaattatcc
      961 ggaaaagctt cctatgtcaa aaactgagct tgataactac ttcaaggaga gaaactggtg
     1021 atctgccagg ttatgttatt atattgtaaa ccatttgaaa tcaataaaat caaatccgga
     1081 ataaaatgaa aaacctgatc aagaaactgc aggaagagaa cagaaaactt gaaacagctg
     1141 aagctgatgc aatacaggct gaaataaata accccgccac cggctccggc cgccgggatt
     1201 tcctgaaaaa gacagccctg ggcggattgt cactcggagg gctgatggcc ttgtcagttg
     1261 aagatacgat agcctttacc acccagaaag tcagaagggc atcaagcccc tctgacctga
     1321 agattaccga tatgcgatac gccacgataa tgaacaggca tatcatccgt atcgacacca
     1381 accagggaat ttacggactg ggcgaggtaa gggatgctgc cgatgtgagg tatgccctct
     1441 ttctcaagag caggatactc gggctgaatc cctgcaatgt tgaaatgatc tttaagatta
     1501 tcagacagtt tggttatcac ggacgacagg gaggtggtgt ctgtgccgtg gaaatggctt
     1561 tgtgggatct caccggaaag gcttacaatg tccctgcatg gcagctgctc ggcggacgat
     1621 accgtgaaaa ggtaaggctg tatgccgaca ctccggatgc aggtgatccg gtcaaatttg
     1681 cggaagtaat gaaaaaaaga gttcaggacc agggatttac atggctgaaa atggaccttg
     1741 gcattcatgt tgttacaaaa atccctgatg ctcttgtcaa ttcaaaattc tgggacgggg
     1801 cagtcgggca atatgatctc agggattaca tgggatacgg aaatacctta catccgttta
     1861 cccaggtcca gataaccgaa agaggccttg caggactggc tgaatacgtt gaaaaggtga
     1921 gagacgaagt aggatatgaa atacccttgt gcgcagatca tttcggacat tttgacctca
     1981 ataattcaat ccgcttcgga agagcaatgg aaaaatacag acttgcctgg cttgaggata
     2041 tggtcccctg gtttgatacc gtaaacaaca aaaagctttc agatgcactg gaaacaccta
     2101 tagcaaccgg agaggacatc tattgcctca gggacggctt caa
//
LOCUS       DAOSPX010000436         2128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_47423_length_2128_cov_3.920405, whole genome shotgun sequence.
ACCESSION   DAOSPX010000436 DAOSPX010000000
VERSION     DAOSPX010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47423_length_2128_cov_3.920405"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /gene="rplD"
                     /locus_tag="PLZ75_14160"
     CDS             complement(<1..380)
                     /gene="rplD"
                     /locus_tag="PLZ75_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOS73586.1"
                     /translation="MELAVLNIQGKETGRKVLLNDSVFGIEPNDHAIYLDTRQFLANQ
                     RQGTHKAKERGEIAGSSRKIKRQKGTGTARAGSIKNPLFRGGGRVFGPKPRDYGFKLN
                     KKVKDLARKSALSYKALSNNIIVL"
     gene            complement(380..1000)
                     /gene="rplC"
                     /locus_tag="PLZ75_14165"
     CDS             complement(380..1000)
                     /gene="rplC"
                     /locus_tag="PLZ75_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOS73587.1"
                     /translation="MQGLIGKKIGMTSLFDEEGKNVACTVLQAGPCVVTQVKTREKDG
                     YSAIQLGFEDKKEKHATKAEIGHFKKASTTPKRKVAEFTGFETDGIKEGEVLTAEIFR
                     SDMWVDVRGWSKGKGFQGVMKRHGFSGVGDTSHGQHNRQRSPGSLGGSSTPSRVFRGM
                     KMAGQTGSRKVMSESLRIMKLMEEENIMIVKGSVPGPNGAYVIILK"
     gene            complement(1022..1327)
                     /gene="rpsJ"
                     /locus_tag="PLZ75_14170"
     CDS             complement(1022..1327)
                     /gene="rpsJ"
                     /locus_tag="PLZ75_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOS73588.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKSTGAVVSGPIPLPTH
                     KKIYTVLRSPFVNKKAREQFQLSSYKRLLDIYSSTPKTIDALMKLELPSGVEVEIKV"
     gene            complement(1330..>2128)
                     /gene="fusA"
                     /locus_tag="PLZ75_14175"
     CDS             complement(1330..>2128)
                     /gene="fusA"
                     /locus_tag="PLZ75_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853959.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOS73589.1"
                     /translation="EEDPTFKVKTDEDSGQTIISGMGELHLEVLINRLKREFNVECNQ
                     GQPQVAYKEAITTSAEFREIFKRQTGGKGKYADITVEMMPADNGIRGLQFLNEVRGGN
                     IPKEYIPSVEKGFREAMSNGPLAGFPLESLKIILKDGSFHPVDSDDLSFEIAAKQAFR
                     KYSGKCHPVLLEPVMSTEVVTPEEYVGDVISDFNKRRGRVEGMESKAGSRVVRAKVPL
                     AEKFGYVTVLRTLTSGRATSTMEFSHYEEVPAEIANRIVENIKGKIL"
BASE COUNT          450 a          574 c          418 g          686 t
ORIGIN      
        1 tccagaacaa tgatattgtt tgataaagcc ttgtaggaca gagctgattt tcttgcaagg
       61 tcctttacct ttttattcag cttgaatccg taatctctcg gtttcggtcc gaatacacgt
      121 ccgcctcccc tgaagagcgg gtttttgata ctgccagcac gggccgttcc tgttcccttc
      181 tgccttttta tctttctgct gcttcctgca atctccccgc gttctttggc tttatgggtg
      241 ccctgacgct ggtttgcgag gaactgcctg gtatccagat aaattgcatg atcatttggt
      301 tctatgccga aaactgaatc attcagcaag acttttcttc ccgtttcctt accctgaata
      361 ttaagaacag ctaattccat tatttcaaaa ttattacata agcaccattc ggacctggaa
      421 cagatccttt tactatcata atattttctt cttccatcag tttcattatc ctaaggcttt
      481 ccgacatcac ttttctgctg cctgtctgtc ctgccatttt cattccccgg aacactcttg
      541 acggtgtgga agaacctccg agcgaacccg gtgatctttg cctgttatgc tgaccatgcg
      601 atgtgtctcc cacaccactg aagccatggc gcttcataac accctggaat ccttttccct
      661 tggaccagcc acgtacatca acccacatgt cggagcggaa tatttctgcc gtaaggactt
      721 ctccttcctt tatcccgtct gtttcaaaac ccgtgaattc tgcaaccttc ctcttgggtg
      781 tggtactggc ctttttaaaa tgtccgattt ctgctttcgt tgcatgtttt tccttcttgt
      841 cttcaaaacc gagctggatg gcagaatatc cgtccttttc ccttgtcttt acctgtgtaa
      901 caacacaagg cccggcctgc aggacagtgc atgcgacatt ttttccctcc tcatcgaaaa
      961 gggaggtcat tccgattttc ttaccaatta atccctgcat ttctataaat ctttactgct
     1021 ttcatacttt tatttctact tcaacaccac tcggcagctc aagtttcata agagcatcaa
     1081 tggtcttggg agtggagctg taaatatcca gcagcctttt gtatgaagaa agctggaact
     1141 gctccctggc ctttttgtta acaaagggtg accgcaggac cgtgtatatc ttcttgtgcg
     1201 tgggcagcgg gattggtccg ctcaccacag caccggttga ctttacggtt tttacgatct
     1261 tttctgctga tttatcgacc aggttatgat cgtaggattt cagtttgata cgaattttct
     1321 gactcatctt taaagaattt ttcctttaat attttccaca attctgtttg ctatttctgc
     1381 aggcacttcc tcatagtgtg aaaactccat tgttgaagtc gccctcccgg aggtgagggt
     1441 ccggagaacg gtgacatagc cgaatttttc ggcaagcggc acctttgccc ttacaaccct
     1501 tgatcctgcc tttgattcca ttccttccac cctgcccctc cgcttgttga agtcgcttat
     1561 gacatcaccg acatattcct cgggagtaac cacttccgtt gacattaccg gttccagaag
     1621 caccggatgg catttgcctg aatattttct gaatgcctgt tttgccgcta tttcgaaaga
     1681 caggtcatcg gaatcaaccg gatgaaaaga tccgtccttc agtattattt taaggctttc
     1741 cagagggaag ccggccagtg gtccgttaga catcgcctca cggaatcctt tttcaacgga
     1801 agggatgtat tccttcggta tattcccgcc cctgacctca ttcaggaact gaagccctct
     1861 tattccatta tccgcgggca tcatctcaac ggtaatatcc gcatatttgc ccttgcctcc
     1921 tgtctgtctc ttgaatattt cccggaattc cgccgaagtg gtgatggcct ccttataggc
     1981 cacctggggc tgaccctggt tgcattcaac attgaactcc ctcttcagcc ggtttatgag
     2041 aacctccaga tgaagctccc ccattccgct gattattgtc tgaccgctgt cctcatcggt
     2101 ttttaccttg aaagtcggat cctcttcc
//
LOCUS       DAOSPX010000437         2114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_47881_length_2114_cov_3.452647, whole genome shotgun sequence.
ACCESSION   DAOSPX010000437 DAOSPX010000000
VERSION     DAOSPX010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47881_length_2114_cov_3.452647"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PLZ75_14180"
     CDS             complement(<1..997)
                     /locus_tag="PLZ75_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS73590.1"
                     /translation="MLKLIRIYYRGNCPVFRLINGHLRPALAGIVFISAAMLYSCGSG
                     GSSTMDETPTRGNIKITVDESFQPLLDTEVSTFMQLYTNATIKARYKPEVDVINDFMR
                     DSVKVIVTSRKLTDNQIKYLRDSLIVARTITFAYDGLALVTNKQNRDTLLKYEQVRDI
                     FLGRAVTWADLDSRSKLGNIRVIFDNNKSGNIRYFRELFEISEALPSNFYAVNSNPEV
                     IEFVSRNPDALGIISVNWISNKNDFRSRSYAGQVNILAISRPMIDDGSYYRPDQGWIY
                     DKSYPFVREVFMITRETFKGLGAGFIQWSTAEQGQRIVLKSGLVPATMPIRLVQIR"
     gene            complement(1097..1333)
                     /locus_tag="PLZ75_14185"
     CDS             complement(1097..1333)
                     /locus_tag="PLZ75_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73591.1"
                     /translation="MNDNRILQQIYAMFGIFMVMFYLGGGIFFLFFADRLLNINKAIL
                     GILGGTFLLYGVYRIFVTYKQITDAFFSKDDDED"
     gene            complement(1426..>2114)
                     /locus_tag="PLZ75_14190"
     CDS             complement(1426..>2114)
                     /locus_tag="PLZ75_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOS73592.1"
                     /translation="DEQSDAIRYQTIYGRISGSVASPTAGLHFTDRVIKELLNRGMQQ
                     TEITLHVGAGTFQPVRAKNIAEHEMHCERYTVTRSTLELLLSKPGKAIAVGTTAVRTL
                     ESLYWLGRKILTGPSGGREPFYTAQWEAYGVNREKETGTEDSLGALLRIMDENGTDMI
                     EASTSLMIIPGYRFRMTDGMITNFHLPRSSLLLLVSAWTGSKWKEIYDFALTHDFRFL
                     SYGDSSLLIK"
BASE COUNT          538 a          483 c          455 g          638 t
ORIGIN      
        1 tccttatctg taccagcctt atcggcatgg ttgccggcac aagtcccgat ttcagaacga
       61 tgcgctgacc ctgttccgcg gtagaccatt ggataaagcc ggctcccagt cccttgaatg
      121 tttccctggt tatcatgaaa acctctctga caaagggata tgatttgtcg tagatccatc
      181 cctgatccgg gcggtaatag gagccgtcat caatcatggg gcgggagatt gccagtatat
      241 ttacctgtcc ggcatagctc ctgctgcgga aatcattctt gttgcttatc cagttcacgc
      301 ttataatacc cagtgcatca gggttcctgg atacgaattc tatcacttcg ggatttgaat
      361 tcactgcata gaaatttgat ggcaaagctt ctgaaatttc aaacagctct ctgaaatacc
      421 tgatatttcc tgacttgtta ttgtcgaata tgactcttat gtttccgagt ttcgatctgg
      481 agtcgagatc tgcccaggta acggcccttc cgaggaatat atccctcacc tgttcatact
      541 tgagaagggt gtccctgttc tgtttgttgg tgacaagggc aaggccgtca tatgcaaatg
      601 ttattgttct ggctacaatg agactgtctc ttaagtattt tatctgatta tcagtcagtt
      661 tcctgcttgt tactatcact ttcacactgt cccgcatgaa atcgtttatc acgtcaactt
      721 cgggcttgta gcgggcttta atcgttgcat tggtatagag ctgcatgaaa gtggatactt
      781 ccgtatcgag aagcggctga aaagattcat caacggttat ttttatattg ccccgggtag
      841 gtgtttcgtc cattgtggaa cttcctccgc tgccgcagga atacagcatt gctgcagaaa
      901 taaaaacaat gccggcaagg gccggacgca ggtgcccgtt gattaagcgg aaaaccgggc
      961 agttccctct gtagtatatc ctgatcagct tcaacataat cttaactcgc aaactctgtc
     1021 ttcagtacca tttattgtca cgcaaagtat atgccaagac ttcttccccg gaactgagca
     1081 aaggccggat gaagattcag tcctcatcat catcctttga aaaaaaagcg tctgttatct
     1141 gtttgtaagt tacgaaaatc ctgtaaactc cgtaaagcag gaaggtacct cctagtattc
     1201 ctaatatagc cttgtttatg tttaaaagtc tgtcggcgaa aaacaggaaa aaaattcctc
     1261 ctcccagata aaacattacc atgaagattc caaacatggc ataaatttgc tgaagtattc
     1321 tgttatcatt cataattagt cactggcaaa taaaagaaaa atcgtgttaa tttcaaaagt
     1381 tttgaaaatg ttattgataa atgaactaaa actgcaggtg aatgtctatt ttattaacaa
     1441 tgaactgtct ccgtagctca gaaatctgaa atcatgagtc agtgcaaaat catatatttc
     1501 cttccattta ctgcctgtcc acgctgacac cagaagaaga agagaactcc tcggaagatg
     1561 gaaattggtt atcatgccat ctgtcatcct gaatctgtat ccggggatta tcatcaggga
     1621 ggttgaagcc tcaatcatgt cggttccgtt ctcatccatg atccgtagaa gtgcgccaag
     1681 tgagtcttct gtaccggttt ccttctcccg gttcacgcca taggcttccc actgtgcggt
     1741 ataaaaaggt tctcttccgc cggaaggtcc ggtaagtatt ttcctgccaa gccagtagag
     1801 gctctcaagg gttctgacag cggttgttcc cactgcaatg gcctttccgg gttttgacag
     1861 caagagctca agggtactgc gtgtgactgt atatcgttca caatgcattt catgttccgc
     1921 gatatttttt gcccttacag gctgaaatgt tccggctccg acgtgaaggg ttatttctgt
     1981 ctgttgcatg cctctgttta gcagctcttt aattaccctg tcggtaaaat gcagtcctgc
     2041 agtcggggaa gcaactgacc cgctgattct tccgtatatg gtctgatagc gtattgcatc
     2101 actttgttcg tctt
//
LOCUS       DAOSPX010000438         2082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_48922_length_2082_cov_4.004440, whole genome shotgun sequence.
ACCESSION   DAOSPX010000438 DAOSPX010000000
VERSION     DAOSPX010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48922_length_2082_cov_4.004440"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PLZ75_14195"
     CDS             <1..1185
                     /locus_tag="PLZ75_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73593.1"
                     /translation="CCKMTGNNAGLSMLGGADTGNLNAYPLFARLVPLIRQYEELRHR
                     NYFSDSVKALLRQPGKEFSLFRDNDGKWRFKPVSYQRHKIAGTGHPTAKWQVNNEFEE
                     QPLKLRIEVLMSVTPYDGSENIMLSGLPEDTAFVIKESAKGVTGRIIPSFGHRAGDGS
                     GIALAAHNSGQVPREGAWLKAEKRFEPALNLEKNKGLGVWIKGDSCGALLNLRTESPN
                     HVSMGARGDHFIKIDFRGWRYFELVEIESSEFNNYIWPDEYFNVYNSYFYTVHFDNID
                     KLQLWYNNLPAGKEVECVIGPVKALPLVPVTIHDPVIRADEESIQFPVKLESGMYLEF
                     NSLEDCKVYGSRGELISEVRMKGEVPLLRKGLNDISFGCRGTKDISSRVQVTVITEGN
                     AI"
     gene            1267..>2082
                     /locus_tag="PLZ75_14200"
     CDS             1267..>2082
                     /locus_tag="PLZ75_14200"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS73594.1"
                     /translation="MKTRIKAMKNRITPSDDSFPEKSGPPESPGKAIPRRSFLRKAGI
                     AAAALGFHQYACSPGRKSVRTPEVPPPVQGFERSPEQDRSRDWIPVSDRKIRVGIIGY
                     GVCRFGADFGFQNHPNVEVVAVSDLFPDRCAALARACRCSKTYPSLEELVKDDGIEAV
                     FVATDAPGHASHVIDALRHGKHVACAVPAVFGSLEDADRLFEEVKSSGLKYMMFETSC
                     YRQDNYSMQHIYRAGGFGELVYSEGEYFHYMEQPIDSYRGWRIGLPPQWYPTHS"
BASE COUNT          582 a          476 c          533 g          491 t
ORIGIN      
        1 tgctgcaaga tgacaggcaa caatgcaggg ctgtcaatgc ttggcggtgc cgacacggga
       61 aacctgaacg cttatccgct gttcgccagg cttgtcccct taatcagaca gtatgaagaa
      121 ttgcgccaca gaaattattt cagtgattca gtaaaggctc ttctccggca gcccggaaag
      181 gagttcagcc ttttccgtga caatgacggt aagtggagat tcaaacccgt ttcatatcag
      241 aggcataaaa tagccggcac cggtcatcca acagcaaaat ggcaggtaaa caatgaattt
      301 gaagaacagc ccctgaaact gagaatagaa gttctcatgt cggtcactcc ctatgacggc
      361 agtgaaaata ttatgctttc cggcctgccg gaagatacag ctttcgtgat aaaggaaagt
      421 gcaaaaggtg ttacaggccg gatcatacca tctttcgggc acagagccgg agacggttca
      481 ggtattgccc tggctgcaca taattccgga caagttcccc gggagggagc atggctgaaa
      541 gcggaaaaaa gatttgaacc ggcactgaat ctcgaaaaga acaaaggact gggtgtgtgg
      601 atcaaagggg attcctgcgg ggcattgctg aaccttcgta ccgaaagccc caatcatgta
      661 tccatgggtg cacgtggcga ccattttatc aaaattgatt tcaggggatg gagatatttt
      721 gaactggttg aaatcgaatc atcggaattc aacaactata tctggcccga tgaatatttc
      781 aatgtgtata actcctattt ctatacggtt cattttgata atatcgacaa gcttcagcta
      841 tggtataaca accttccggc aggaaaggaa gttgaatgtg tcattggccc ggtaaaggca
      901 cttccgctgg tcccggtaac catccatgac ccggtaatca gagcagatga ggaaagcatt
      961 cagtttcccg taaagctgga atcaggaatg tacctggagt ttaattcatt ggaggattgt
     1021 aaagtttatg gttcacgggg agaactgatc agtgaggtca ggatgaaagg cgaagtcccg
     1081 cttctgagaa aagggctgaa tgacatatca ttcggttgca ggggaacaaa ggatatcagt
     1141 tcccgggttc aggttacggt cataactgaa ggcaatgcta tataatcaca gtataagatt
     1201 atttgatgct acataatcac ctcaatccat aaaatccacc gcaaacccat gaaaaaaaca
     1261 gcatccatga aaacaagaat caaagccatg aaaaaccgga ttacaccttc tgacgattca
     1321 tttcctgaaa aatccggtcc cccagaatcc ccaggaaaag ctattcccag gcgttctttt
     1381 ctcaggaaag cgggaattgc tgcggctgca ctgggttttc accagtatgc ctgttccccg
     1441 ggcagaaaaa gcgtccggac accggaggtg ccgcctcccg ttcagggatt tgaaagaagt
     1501 cctgagcagg accgttcacg tgactggatt cctgtatcag acaggaaaat ccgggtcgga
     1561 ataatcggct atggtgtttg cagattcgga gccgatttcg gattccagaa ccatccgaat
     1621 gtagaagtgg ttgccgtcag cgacctcttt cccgacaggt gtgctgctct tgccagggca
     1681 tgccgctgtt caaaaaccta tccttccctt gaagaactgg tgaaagatga cgggatagag
     1741 gcagtattcg tggcgactga tgccccgggc catgcctctc atgtaattga tgccctgcgc
     1801 cacgggaagc atgttgcctg tgcagtgccc gccgttttcg ggtcgcttga ggatgcggac
     1861 agattgtttg aagaagttaa atcgagcgga ctgaaatata tgatgttcga aacttcctgc
     1921 tacaggcagg acaactattc aatgcagcat atctacaggg ccgggggatt cggagagctg
     1981 gtctattcgg aaggcgaata ctttcactac atggaacaac ccattgattc atacagggga
     2041 tggcggatcg gtctgccgcc gcaatggtat cccacacatt cc
//
LOCUS       DAOSPX010000439         2080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_48987_length_2080_cov_4.101728, whole genome shotgun sequence.
ACCESSION   DAOSPX010000439 DAOSPX010000000
VERSION     DAOSPX010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48987_length_2080_cov_4.101728"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLZ75_14205"
     CDS             <1..334
                     /locus_tag="PLZ75_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73595.1"
                     /translation="PEENISYTVNNDISHGDLKEYQLGLAPKVSIKLADLIKNNPEAT
                     SDKEMAKTFSGIKGIGEARAGKIYDTFKKKGKITNGKADIGIGLVALNRQNKGKIITS
                     GEFSGPWI"
     gene            363..>2080
                     /locus_tag="PLZ75_14210"
     CDS             363..>2080
                     /locus_tag="PLZ75_14210"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HOS73596.1"
                     /translation="MRRILRKYRSATRRSAGTSQFKQASPLFFDFNSGGSFFQGHSSG
                     TMIDRGSDSDDKKRENNQLNRSQISKYNNAPGNVTAYINTLKEGTSLSRKSRQFFEPK
                     FGADFSRVRIHNDKDSAEAAASVHAKAFTYKNHIVFNNGEYDPDTEKGKKLLSHELTH
                     VIQQDNSIKRVETEHEEEEKIQKKDSVTSDTVLAKGPDKEASNRQSDLMPEVSQGSFD
                     TCGAASLVAAIMIHDKQLSTSSVPDNSGFIAAANIVLSYFTMNRESVISGLAAGRKIP
                     AEQAEKIFDFIKNELLRMRNDSLKPGAVVQQYDFQILSVAIYALHISPASGLSQAAIW
                     NITNMLGIKTESKGGLRSYSEIINHSLLKALAPGQVAQIGWYVSRGGGKYGLHALIIG
                     RLNNGTWYLYDQGTDPPARFTASSLPELDAVIRAASGSGSYWLFTGSLSDFSMFLLGG
                     WTGINVLGKTADVLQASANLLKTGTHLAEIDAGYLTIGSDVHVDAFQAEFYDFQAAQS
                     AASGLSGQGGAIIEMPEGRYLLFSTTLVSDANRNETSIDISGGGEFTEKRFLHAWLQL
                     RSKKGTG"
BASE COUNT          650 a          402 c          498 g          530 t
ORIGIN      
        1 cccggaagaa aacataagtt atacggttaa caatgatatc agtcacggtg atttgaaaga
       61 atatcagctg gggctggccc cgaaagtaag tataaaactt gctgacctga taaaaaataa
      121 tccggaagcc acttcagaca aagaaatggc aaaaacattt tccggaataa aaggtatcgg
      181 ggaggcaaga gcagggaaaa tatatgacac gtttaagaaa aaggggaaga tcaccaacgg
      241 aaaggctgat atcggaatcg gccttgttgc actgaaccgt cagaataaag gcaaaataat
      301 aacatctggt gaattttcag gtccgtggat ctgaacatga ataaatcaaa ttcaggaggc
      361 taatgagaag aatccttcgc aaatacagat ctgctacccg caggagtgcc ggaacgtcac
      421 aatttaaaca agcttcacct ttattttttg attttaattc aggaggatca tttttccagg
      481 gtcattcttc cggaacaatg attgaccggg gaagtgattc tgatgataaa aaacgggaaa
      541 acaatcagtt aaatcgttct cagatttcaa aatataataa tgctcccggt aatgtcactg
      601 cctatattaa cactctgaaa gaggggactt cattatcaag gaaatcgcga caatttttcg
      661 aacctaagtt tggagctgac ttcagcaggg tcaggattca taacgataag gattctgcag
      721 aagcagctgc atctgtccat gcaaaagctt ttacttataa gaaccacatt gtttttaata
      781 acggggagta tgatccggac actgaaaagg gcaaaaagct cctgtcgcat gaacttacac
      841 atgtaatcca gcaggataat tctataaaaa gggttgaaac cgaacatgag gaagaggaaa
      901 aaattcagaa gaaagattca gtcacgagcg atactgtatt agcaaaaggg cctgataaag
      961 aagcgagtaa caggcagtct gatttaatgc cagaagtatc acagggtagc tttgatacct
     1021 gtggcgccgc atcccttgtt gcagccataa tgatacacga caaacagctg agtacttctt
     1081 ctgtgcctga caacagcgga ttcattgctg ctgccaatat tgtcctgtcg tattttacga
     1141 tgaaccgtga aagtgttata tcaggtcttg ctgccggccg taagatcccc gcagaacagg
     1201 cggaaaagat atttgacttt ataaagaatg agctgctcag gatgcggaat gacagcctta
     1261 aaccaggcgc ggttgttcaa cagtatgatt ttcagatatt atccgttgct atctacgcct
     1321 tgcatatctc accggcaagc ggattatcgc aggcagctat atggaacata actaatatgc
     1381 tgggtataaa aacagagtca aaaggaggac ttagatcata cagtgagatc ataaatcatt
     1441 ctttactgaa ggcattagct cccggacagg tggcacagat cggctggtat gtaagcaggg
     1501 gcgggggcaa atatggcctg cacgctttaa ttattggcag actgaacaat ggtacatggt
     1561 acctgtatga tcagggcacg gatccgcctg cacgttttac cgcttcttct ctgcctgaac
     1621 tggatgctgt aattagagct gcctcaggat caggatcata ctggctgttt acaggatcat
     1681 tatctgactt ttctatgttc ctgctgggag gatggaccgg gatcaatgtt ttgggaaaaa
     1741 ctgctgatgt acttcaggcc tcagcaaatc tgcttaaaac gggaacccat ctggctgaaa
     1801 ttgatgctgg ttatctgacg attggcagtg atgttcatgt tgatgctttt caggcagaat
     1861 tttacgattt tcaggcagca cagtcagctg catcaggatt atcagggcag ggtggtgcaa
     1921 ttattgaaat gccggaaggc cggtacctgt tattttctac cacgttggta agcgatgcca
     1981 acaggaatga aaccagtatt gatatctcag gtggtggcga gtttacagaa aaaagatttt
     2041 tgcatgcatg gctgcaactg agaagcaaaa agggtaccgg
//
LOCUS       DAOSPX010000440         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_49085_length_2077_cov_3.439664, whole genome shotgun sequence.
ACCESSION   DAOSPX010000440 DAOSPX010000000
VERSION     DAOSPX010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49085_length_2077_cov_3.439664"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLZ75_14215"
     CDS             complement(<1..681)
                     /locus_tag="PLZ75_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS73597.1"
                     /translation="MEKKLYIETYGCQMNIADSDVIASVLSGTGHRLVKDPGEAGLIL
                     INTCSVRENAEQRVLNRLKQLRHLKKKNPGLIVGLAGCMAERLREKLFDTEDLIDIVA
                     GPDSYRDLPSLVAEAASGKKAMNVQLSRCETYSDILPHSGHDGISAFISITRGCNNMC
                     SYCIVPYVRGAERSRNPESILGEARMHFESGFREVTLLGQNVDSYKWVSGNETIEFHA
                     LIAAVAGID"
     gene            844..1587
                     /locus_tag="PLZ75_14220"
     CDS             844..1587
                     /locus_tag="PLZ75_14220"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOS73598.1"
                     /translation="MSGHSKWSTIKRKKGAADARRGKVFTKIIKEIIIAARDGGGDPD
                     SNSRLRLAIQNAKGVNMPKDNIERAIKKAVGSDAEHYAETTFEGYAPNGIAVFVECLT
                     DNNNRTVASVRSVFNKYGGSLGTNGSLSYLFDTKGIFTIRNEGLSLEELELEMIDAGS
                     EEFEIEEETITITCAKEDFGNVSKKLGEMEIEAIEAGLKRIPNDTKTLDVETAKKVLK
                     FIETLEDDDDVQNVYHNLEMTDELAEALE"
     gene            1632..>2077
                     /locus_tag="PLZ75_14225"
     CDS             1632..>2077
                     /locus_tag="PLZ75_14225"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOS73599.1"
                     /translation="MARKALIFLMLFSFSAAGTMAQGFIRTIDLMKRSDEKNSGRLTI
                     VQDRGIDTLISRSIMANSKLKTMEGSPGIQGFRIQIYYSSVRNAREESARARAVFINK
                     FPDIVSYVQYQEPGYFMVRVGDYRTKTELYKDLIAIRRVFPNAYHVP"
BASE COUNT          591 a          449 c          518 g          519 t
ORIGIN      
        1 atcaatgccg gccactgcgg caatcagggc atgaaattca atggtttcat tgcctgagac
       61 ccatttgtag gaatccacat tctgcccgag cagtgtgact tccctgaagc ctgattcaaa
      121 atgcatcctt gcctccccga gtattgattc agggttcctg ctgcgttccg cccctcttac
      181 ataggggaca atgcagtatg aacacatatt gttgcatccc ctggttatgg atatgaatgc
      241 cgaaatgccg tcatggcctg aatgaggcaa tatgtcggaa taagtttcgc agcgtgaaag
      301 ctgtacattc atggcttttt tgccagaagc cgcctctgct acaagtgagg gaagatcacg
      361 gtagctgtcg ggaccggcga caatatcaat gagatcctca gtatcaaaca gcttttccct
      421 gaggcgttcc gccatgcacc ccgccagacc cacaatcagc ccgggattct tctttttaag
      481 atgcctgagc tgcttcagcc tgttcaggac acgctgttcg gcattctccc tgacagaaca
      541 ggtattgata agtatcagtc ctgcttcgcc cggatccttg acgagccggt gccctgttcc
      601 ggaaagaact gaagcaatga catcgctgtc ggcaatgttc atctggcagc cataggtttc
      661 tatatatagt tttttttcca tattgtactg tttttatccg gcaccccggg cttaccgccg
      721 gagcgtcgcg gaatattccc ctgccgggag ccgctttttt tcggactaca aaagtacata
      781 acctggatga agtattaaag atttaattaa ttttgtatac gctaaaatca taaaacctat
      841 tgtatgtcag gtcacagtaa atggtcaaca attaaaagaa agaaaggggc agcagatgcc
      901 cggagaggaa aggtttttac caagatcatc aaggaaataa ttattgctgc aagggatggc
      961 ggcggtgatc ctgattcaaa ttccaggctg agactggcga tacagaatgc caagggtgtc
     1021 aatatgccga aggacaatat tgaaagagcc ataaagaaag ctgttggttc tgatgccgag
     1081 cattatgcgg aaactacttt tgagggctat gcaccgaatg gtattgccgt atttgtcgaa
     1141 tgcctcaccg ataacaacaa caggacggtg gcatcggtca ggtcggtgtt caacaagtac
     1201 ggcggcagtc tgggtacaaa cgggtcgctc agctatcttt ttgacacgaa gggtattttt
     1261 accatccgca atgaaggcct gtcccttgag gaactggaac tggaaatgat agatgccggt
     1321 tcggaagaat ttgagattga agaagaaacc ataacaatca cctgtgccaa ggaagatttc
     1381 ggaaatgtca gcaaaaaact gggagaaatg gaaatagagg ctattgaagc cggattgaaa
     1441 agaatcccga atgatacgaa aacgctggat gttgaaacgg cgaagaaagt gctgaaattc
     1501 attgagaccc ttgaagatga tgatgatgtc cagaatgtgt atcataatct ggaaatgact
     1561 gacgaacttg cggaggcact ggaataaaaa tcagcactaa tattgtacat ttgtccaaaa
     1621 aattgaagcg gatggcaaga aaggccttga tatttctaat gctcttttcg ttttcagcag
     1681 ccggcaccat ggcccagggc tttatccgga ccatagacct catgaagcga tccgatgaaa
     1741 aaaattccgg aaggcttacc attgttcagg acagggggat agatacgctt atcagcaggt
     1801 cgattatggc aaacagcaag ctgaaaacca tggagggctc acccgggatc cagggtttca
     1861 gaatacagat ttattacagt tcggtgagaa atgccaggga agagtcggca cgggccagag
     1921 ctgtattcat caacaaattc cccgatattg tttcctatgt tcagtatcag gagccgggtt
     1981 atttcatggt gagggtgggt gattacagga caaaaaccga attatacaag gatctgattg
     2041 caataaggcg ggtgtttccg aatgcctacc atgttcc
//
LOCUS       DAOSPX010000441         2055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_49857_length_2055_cov_2.664000, whole genome shotgun sequence.
ACCESSION   DAOSPX010000441 DAOSPX010000000
VERSION     DAOSPX010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49857_length_2055_cov_2.664000"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLZ75_14230"
     CDS             <1..256
                     /locus_tag="PLZ75_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73600.1"
                     /translation="RISANHNRPDRRLQITISIIDVSGRTIRIIKEETWSGGYRLSPL
                     LWDGNTDGGERAGKGIYFCRLTVRTDDGEEASGTVRIIIL"
     gene            325..1500
                     /gene="porV"
                     /locus_tag="PLZ75_14235"
     CDS             325..1500
                     /gene="porV"
                     /locus_tag="PLZ75_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOS73601.1"
                     /translation="MKNRLIGIFLVTVMLSGTINLYSQPDGELNAIQTVVPFLTIAPD
                     SRAGAMGDAGVATSPDVYSMHWNPAKFAFIEEKAGVGISYSPWLRNLVPDINIAYLSG
                     YYKLDSRQVLSGSLLYSSLGDVDFTDQYGNLERTFTPNEWSLDVAYSRLFTDRLSGGI
                     AFRMIYSNLTGGSYSGGEATKAGISVAADISLYHHTDMTILNKDSRLAFGVNFSNIGS
                     KMSYTDAQTSDFIPMNMRLGTALTMNLDTYNKITLTTDINKLLVPTPPRYAITNPDSI
                     IAGKDPNVSVPVAIFQSFYDAPGGFREELREYTLSVGTEYWYNEQFALRAGYFHENET
                     KGNRKYFTAGAGFRLSGFTVDFSYLMPTVQNHPLARTLRFSIAFDISSAGKKASSNI"
     gene            1497..1979
                     /gene="ispF"
                     /locus_tag="PLZ75_14240"
     CDS             1497..1979
                     /gene="ispF"
                     /locus_tag="PLZ75_14240"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOS73602.1"
                     /translation="MNLRTGLGIDFHSFGTGPEFWLGGVLIPFSKGCIAHSDGDCLLH
                     AICDAMLGAAALGDIGHHFPDNSPEFKNISSRVLLTRTNELIREKGYCLVNIDSTVCL
                     EEPRISPYIPRMKSVISAILSLDSGSISIKATTTEKMGFTGRGEGVAALASVLLKSLA
                     "
BASE COUNT          585 a          509 c          489 g          472 t
ORIGIN      
        1 aagaataagt gcaaaccata accggcccga ccgcaggctt caaattacca tcagcataat
       61 tgatgtgagc gggagaacca tcagaataat aaaggaggaa acctggtcag gcggttaccg
      121 tctgtcaccg ctgctgtggg acggaaatac cgatggcggg gaaagagccg gaaaaggcat
      181 ctatttctgc aggttaacag tccgtacgga cgatggcgag gaggcttcgg gaacagtcag
      241 aataatcatt ttataaacaa tacataataa gtcggagaaa atttagtttt atatttacat
      301 ttttcttgaa tgaaacgtat cggaatgaaa aacagactaa tcggaatatt cctggtgaca
      361 gttatgctgt caggaaccat taatctttac agccagccgg acggtgagct caatgccatt
      421 cagacggtgg tgccttttct gacaattgca cccgattcaa gagccggggc aatgggtgat
      481 gccggtgttg caacatctcc cgatgtttac agcatgcact ggaatccggc taaatttgca
      541 ttcatcgaag aaaaggcagg tgtgggaata tcatattccc cgtggctgag aaacctggtc
      601 ccggatatta acatcgcata cctcagtggt tattacaaac tggacagcag gcaggtcctc
      661 agcggaagcc ttctgtattc atcactcggg gatgttgact ttacagatca gtacggaaat
      721 cttgagagaa cattcacccc gaatgaatgg tcacttgatg tggcatattc aagactgttc
      781 actgacaggc tctcgggagg aatcgcattc cgtatgatat actccaacct gaccggagga
      841 tcctattccg gcggtgaagc tacaaaagca ggaatctcgg ttgctgccga catttccctg
      901 taccatcata cggatatgac aatcctgaat aaagacagca gacttgcttt cggagtcaac
      961 ttctccaata tcggatcaaa aatgagctat acggatgccc agacttccga tttcataccg
     1021 atgaacatga gactgggaac tgcactgacc atgaaccttg acacctacaa caaaataacg
     1081 ctcaccacag atataaacaa gcttcttgtt cccacacccc cgcgttatgc tatcaccaat
     1141 ccggacagta tcatagccgg aaaagatccc aatgtttcag tgcccgtagc cattttccag
     1201 tccttctacg acgccccggg cggtttcagg gaagaattgc gggaatatac cctgtcagtg
     1261 ggaacggaat actggtacaa tgaacaattc gcactgagag caggctattt ccatgaaaac
     1321 gaaaccaagg gaaacagaaa atacttcact gcaggtgcgg gattcagact gagcggattc
     1381 acagtcgact tctcatatct tatgccgacc gtgcagaatc atcccctggc cagaacactg
     1441 cgtttttcca ttgcattcga catcagctcc gcagggaaaa aagccagttc aaacatatga
     1501 atctcagaac cggcctggga attgacttcc acagcttcgg aacaggcccg gagttctggc
     1561 tgggaggcgt tctgataccc ttctccaaag gctgcatagc ccattccgac ggtgactgtc
     1621 ttctgcatgc catatgtgat gccatgctcg gtgcggcagc actcggcgac atcggccatc
     1681 attttccgga taacagccct gaatttaaaa acatcagcag cagggttctc cttacaagga
     1741 caaatgagct gatcagggag aaaggatatt gccttgtcaa tatcgacagt acggtctgcc
     1801 tcgaagaacc ccgtatctca ccgtacatac ctcggatgaa gtcggtgata tccgcaatac
     1861 tcagcctgga tagcggaagc atatccataa aggccacaac aaccgagaaa atgggattta
     1921 ccggccgcgg tgaaggagta gcggcgctgg cttcagttct tctgaaatcc cttgcctgac
     1981 ctgatacgga gaccgggatc cggtcctgac atatatacac aaacagcgga ttctcacagc
     2041 gtggtatccg ccggc
//
LOCUS       DAOSPX010000442         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_51068_length_2021_cov_4.423194, whole genome shotgun sequence.
ACCESSION   DAOSPX010000442 DAOSPX010000000
VERSION     DAOSPX010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51068_length_2021_cov_4.423194"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLZ75_14245"
     CDS             complement(<1..817)
                     /locus_tag="PLZ75_14245"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOS73603.1"
                     /translation="MKKFIISSFLILTMLFLGIRCSEDFLTPKPLSFYAPENSYIDAN
                     GLNSALIACLRNLRREYYGNMSPFVSEIIFSDQGCSGTTDKTGVHMDLPAQILPDESM
                     IRGDQTQLGRYWTENGYNRIQYANVVINRIDEATWKSEEERNNILGKAYFHRANVYYR
                     MVHQYGDVPLILEEIKKPRLDFYTCTRESILEKCKKDLEFAAQWVYAECPIGDVNKAA
                     CNHLLTKVNLSLGKFDDAVASASAIINDGIHELMTTRFGVDKNMIINWANAAIR"
     gene            complement(829..>2021)
                     /locus_tag="PLZ75_14250"
     CDS             complement(829..>2021)
                     /locus_tag="PLZ75_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS73604.1"
                     /translation="FWSLAGGWTISEESFFNVAWIDQLKLRTSYGTNGNRGVGIYDAL
                     SDLGTGKFVMITNGTPGYTSLLYTNRMANSSLKWERTSAFNVGLDLAVLKGRIRANIE
                     GYYMITKDLLIPRQLPNITGYASVMANLGQVDNKGIELTLNSINIGTKDFTWSTDLSM
                     AHNRNKIVHLYGDYVSDPVTGKMVEQDDIINKWFIGHAIDQIWDYKTLGIWQQNEAAE
                     AAAYSRSPGDYKLEDVNKDGYYTNADKQFQGYKNPVVRLTFRNVVQYKNLELVVKMYS
                     NLGHYRANNYVRNGEAFYDRSTYYNVPYWTPENPGNTWARIDSYKTGFDVWQNNSFVR
                     IDNVALSYNVPNKFLNKFKIAGCRFSLVSENSYVIAPWWSWMDPEYNDYSPSNLSFKI
                     NVTL"
BASE COUNT          512 a          443 c          407 g          659 t
ORIGIN      
        1 gcctgatcgc cgcattggcc caatttatga tcatattctt gtccacacca aaacgagttg
       61 tcattaactc atggattcca tcgtttatga ttgcacttgc tgatgctact gcatcatcaa
      121 actttcccag cgacaggttc acttttgtca gaagatgatt gcatgcagcc ttgttgacat
      181 caccaatggg acattctgca tatacccatt gtgcagcaaa ctccagatct tttttgcatt
      241 tctcaaggat actttccctt gtacaggtgt aaaagtcaag tctcggtttc tttatttcct
      301 ccaggatcag ggggacgtca ccgtattggt gaaccatacg gtaatataca tttgcacggt
      361 gaaaataggc ctttcccaga atattatttc tttcttcctc acttttccag gtggcctcat
      421 cgatcctgtt tataacaaca ttggcgtact ggattctgtt atagccgttt tcagtccagt
      481 agcggcctaa ttgtgtctga tcaccacgaa tcatgctttc atcgggaagt atctgggccg
      541 ggagatccat atgaacgccg gttttatcag tcgtgccgct gcagccctgg tccgagaata
      601 ttatttcgga tacaaaaggg gacatgtttc cgtaatattc tcttctgaga ttgcggaggc
      661 aggcaataag ggctgaattc agtccgttgg catcaatgta cgagttttca ggtgcataaa
      721 acgacaatgg ttttggtgta agaaaatctt cggaacatct tattccaagg aaaagcattg
      781 tcaatatcag gaatgaggat ataatgaact ttttcataat tcttggcttt aaagagttac
      841 attaatttta aatgacaggt ttgacgggga atagtcattg tattccggat ccatccatga
      901 ccaccagggt gctatcacat atgaattttc ggataccagt gagaatctgc aacccgctat
      961 tttaaattta ttcagaaatt tattcggcac gttataggat aaggccacgt tatcaatcct
     1021 tacaaatgaa ttgttttgcc atacatcgaa tccggtcttg taactgtcta tccttgccca
     1081 tgtgttacct gggttttcag gtgtccagta tggcacattg taataagttg acctgtcgta
     1141 aaatgcttca ccgttcctta catagttgtt tgcccggtag tgccccaggt tggaatacat
     1201 cttgacaaca agctcaagat ttttatactg gacaacattt ctgaatgtaa gtctgacaac
     1261 aggatttttg taaccctgga attgcttgtc ggcattggta taatatccgt ctttgttaac
     1321 atcttcaagc ttatagtcac ccggcgatct tgaataggct gcagcttcag ccgcttcatt
     1381 ctgttgccag attcccagtg tcttgtaatc ccatatctgg tcaatcgcat gaccgataaa
     1441 ccacttgttg attatatcat cctgttccac catttttcct gttacaggat cgctgacata
     1501 atcaccgtag agatggacta ttttatttct gttatgtgcc atcgaaaggt ctgttgacca
     1561 tgtaaagtcc tttgttccta tattgattga attcagggta agttcaattc ctttattgtc
     1621 aacctgtccc agattggcca ttacgcttgc ataaccggtg atgttgggca attgtctggg
     1681 aatcagcaga tctttggtta tcatgtaata accttcaata ttagctctta ttctgccttt
     1741 aagtacagcc agatcaagtc ctacattaaa ggcacttgtc cgttcccatt tcaggctgga
     1801 atttgccatt ctgtttgtat aaagcaggga agtatatccg ggtgttccgt ttgttatcat
     1861 tacgaatttt cccgttccca gatctgaaag agcatcatat atgcccacgc cacggttgcc
     1921 gttggttccg tatgatgttc tgagtttcag ctggtcaatc caggcgacat taaaaaagct
     1981 ttcctcactt attgtccatc ctccggcaag tgaccagaaa g
//
LOCUS       DAOSPX010000443         1967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_53174_length_1967_cov_3.489540, whole genome shotgun sequence.
ACCESSION   DAOSPX010000443 DAOSPX010000000
VERSION     DAOSPX010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53174_length_1967_cov_3.489540"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1279)
                     /locus_tag="PLZ75_14255"
     CDS             complement(248..1279)
                     /locus_tag="PLZ75_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOS73605.1"
                     /translation="MKALVLEEYNRLVYRDWPDPEIADNEVLVRIRAAAICGSDVHGM
                     DGSTGRRIPPVIMGHEASGIIEETGSEVRNMKKGDRVTFDSTIYSPADWYSLKGLYNL
                     SDNRMVLGVSTEEYRRHGAFAEYLNVPEHILYRLPDDVSYTQAAMVEPLAVAAHAAGL
                     ASPSWNDSVAVIGSGMIGLSLIQVLRSMGCGKIFAVDTDPFKLGLALRLGADQAFDPR
                     EAPVSDSITELTAGRGADLVFEAVGTGETLSTAVASVRKGGTVVLVGNLSPEAIIPLQ
                     NVVTRQIRLQGSCAICGEFPAVLDMMARGEINTDPLISAEAPLSEGAEWFRRLYAREP
                     GLLKVVLHP"
     gene            complement(1295..>1967)
                     /locus_tag="PLZ75_14260"
     CDS             complement(1295..>1967)
                     /locus_tag="PLZ75_14260"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOS73606.1"
                     /translation="KLSSGEWTTPFDPVYSQHEGSDYTEGNAWQYLWLVPQNVEGLIG
                     LLGGSDAFADKLEKLFMVEEGVSGKEASPDISGLIGAYAHGNEPGHHTSFLFNYAGRP
                     WRTQELNRQIQTTMYTNAPDGLCGNEDCGQMSAWYVFSALGFYPVNPADLKYQFGSPL
                     VNNAKIEVAPGKYFTVKAPAASVDNKYIHKVLLNGRELDRTYITHQEIMDGSTLEFVM
                     SPVRK"
BASE COUNT          456 a          554 c          459 g          498 t
ORIGIN      
        1 acggcagcaa aacgtgaacc gggaaggttt gcgagtgccc gggcatgaat agctgtcact
       61 tttccgatac ccacaacggc agtattcagt ttttccttca ttcttaagcc atacatttga
      121 agtttaaatt gaaattcagg atccaacggt ttcgtcaggc tcccgtcaga caaagataat
      181 cacccgccgg cagtacttcc gggttacgga cacacaggtt tcaggttcgg gttcctgttc
      241 ctcatgatca gggatgaaga accactttca acagtcccgg ttctctggca taaagtctcc
      301 tgaaccactc tgcaccctcg gaaagcgggg cttctgcgga gataagagga tcagtattta
      361 tttctccccg ggccatcatg tcaagaactg cgggaaactc cccgcagatg gcacagcttc
      421 cctgaagcct tatctgtcgg gtcaccacat tttgcagggg aataattgcc tcaggggata
      481 aattcccgac aaggacaacg gttccgcctt tacgtacgga agcgactgcg gttgacaaag
      541 tttcacccgt ccccactgcc tcaaaaacca gatccgctcc ccggcctgca gtcagttcag
      601 taatactgtc actgacaggt gcctcccgtg ggtcaaaggc ctgatccgcg ccaagccgca
      661 gagccagtcc aagcttaaac ggatcagtgt caacggcaaa aatctttccg catcccattg
      721 atctcagtac ctggataagt gataatccta tcatgccgct gccgatcacg gcaacggaat
      781 cattccacga gggagaagcg agcccggctg catgggcagc aacggcaagg ggttcaacca
      841 tggcagcctg tgtatatgag acatcatcgg gcagccggta caggatatgc tccggcacat
      901 tcaggtattc cgcaaatgca ccatgccttc tgtattcttc cgtggagaca cccagcacca
      961 tcctgttgtc gctgagattg tacaggcctt tcagggaata ccagtcagcg ggggaataga
     1021 tggttgaatc gaaggttacc ctgtcaccct tcttcatatt cctgacctca cttcctgtct
     1081 cctcgatgat tccggaagct tcatgcccca ttatgaccgg aggaattctc ctgcccgtac
     1141 ttccgtccat tccatggaca tcactgccac agatggcggc agccctgatt cttacaagaa
     1201 cctcgttgtc tgcgatctcc ggatcgggcc agtcacggta aaccagtctg ttatattcct
     1261 caaggacaag agctttcatt ctcgggtata ttttttattt cctgaccgga ctcatgacga
     1321 actccagggt gcttccgtcc attatttcct ggtgggtgat atatgtccgg tccagttccc
     1381 tgccattcag aagcacctta tggatatact tgttatccac tgaggcagcc ggggccttca
     1441 ccgtgaaata ttttcccgga gccacttcta tttttgcatt attgacaagg ggtgatccga
     1501 actggtattt caggtcggca ggattcaccg ggtaaaatcc cagggcgctg aatacatacc
     1561 atgccgacat ctggccgcaa tcctcatttc cgcataatcc gtccggtgca ttggtataca
     1621 tcgtggtctg tatctgacgg ttcagttcct gagtcctcca gggtcttccg gcatagttga
     1681 agaggaaact ggtatggtgc cccggttcat ttccatgtgc ataagctcct atcagtcctg
     1741 agatgtcggg agaagcttcc tttccgctta caccctcctc aaccatgaaa agcttctcaa
     1801 gcttgtccgc aaaggcatca ctgccgccaa gaagcccgat cagtccttca acattctgcg
     1861 gaacaagcca caggtactgc caggcattgc cttctgtata atcgcttccc tcatgctgtg
     1921 aatataccgg atcgaaaggt gttgtccatt caccgctgct cagtttt
//
LOCUS       DAOSPX010000444         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_55526_length_1907_cov_3.182505, whole genome shotgun sequence.
ACCESSION   DAOSPX010000444 DAOSPX010000000
VERSION     DAOSPX010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55526_length_1907_cov_3.182505"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLZ75_14265"
     CDS             <1..863
                     /locus_tag="PLZ75_14265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOS73607.1"
                     /translation="PVVEELIHTSAEQPDPLHEKAFSPVKGIVHRYPDRVLFTVTQVC
                     SNYCRYCTRSHSVGRLDRLGKQDFEKAFSYIGAHSEIRDVLVSGGDPLTLSDEVLDYI
                     LFNIRRIEHVEIIRIGTRVPVVLPQRITDKLLAILRKYHPLYISLHFSHPAEITPECT
                     RACTLLADNGFPLGSQTVLLKGINDNIAVMRELMHKLLKIRVKPYYLYQCDLIPGSGH
                     FRTSVRKGIEIIKGLRGFTSGYAVPTYVIDAPGGGGKIPLLPDYVVEHGSEKIVLRNY
                     QGVLCEYPEK"
     gene            870..1841
                     /locus_tag="PLZ75_14270"
     CDS             870..1841
                     /locus_tag="PLZ75_14270"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73608.1"
                     /translation="MKIGLTYDLRSWYLDRGYSMEDTAEFDRQETVDAIDGALCRMGF
                     ETEAVGNCFQLIEALSSGKRWDLVFNIAEGLYGDGRESVVPAILDQYRIPYVFSGPVT
                     MGVSLNKYITRLIVESAGIPVSPGILVSSAEETENLTLEYPLFVKPVSEGTGKGITEK
                     NLLNSPAELKEMAGYLITRFGQPALVEEYLPGREFTVGVVGTGSDAMVIGGMEIECAG
                     NNPYSVEFKENYREYCQYIPIAEEFSDECSKIALDAWKAIGAADAGRIDLKADRKGRI
                     CFMEVNPLAGLHPVHSDLPILAGMRGIDFQTLIEMIVKSSLKRHNLV"
BASE COUNT          529 a          412 c          478 g          488 t
ORIGIN      
        1 ttcctgttgt tgaggaactg atccatacct ctgccgagca acctgatccc ctgcacgaga
       61 aagctttctc cccggtaaag ggtattgtgc accgatatcc cgacagggtc ctcttcaccg
      121 tgacccaggt ctgttcaaat tactgcagat actgtacccg ttcacattcc gtgggcaggc
      181 ttgacaggct cgggaaacag gatttcgaga aggcattcag ctatataggg gctcacagtg
      241 aaataaggga tgtccttgtc agtggcggtg atccgcttac tctcagcgat gaggtgctgg
      301 attatattct gttcaatata agaaggattg aacatgtcga gataatacgt atcggaacaa
      361 gggttcccgt tgttctgcca cagcggataa ctgacaaact gcttgccatt ctcaggaaat
      421 accatccgct gtatatcagt cttcatttct cacatccggc agaaataacc ccggaatgca
      481 ccagggcatg tacactactg gctgataacg gatttccgct gggcagtcag acagtccttc
      541 tgaaaggaat caatgacaat atcgccgtta tgagggaact catgcataaa ttattgaaaa
      601 taagagtaaa accctattat ctgtatcagt gtgacctcat tcccggttcg ggccatttca
      661 gaacaagtgt cagaaaaggc attgagataa taaaaggcct gagggggttt acaagcggct
      721 atgccgttcc gacttacgtg atcgatgcac cgggcggagg cggtaaaatc cctctcctcc
      781 ccgattatgt ggttgaacat ggcagtgaaa aaattgtgct tcgcaattat cagggcgttt
      841 tgtgcgaata tcctgaaaaa taatattcaa tgaaaatagg cttgacttat gacctgcggt
      901 cgtggtatct cgaccgcggg tattctatgg aagatactgc agaatttgac aggcaggaaa
      961 ctgttgatgc aattgacggg gcattgtgca gaatgggatt tgaaacagaa gcggtgggta
     1021 actgttttca gctgattgaa gcactgtctt ccggaaaacg gtgggatctg gttttcaata
     1081 ttgccgaagg actgtacggc gatggcaggg aatcagttgt gccggccatt cttgatcagt
     1141 accgcatacc ctatgttttc agcggcccgg tgaccatggg tgtttccctc aataaataca
     1201 tcacccggtt aatcgtggaa tcagcaggaa ttccggtaag tccgggcata cttgttagtt
     1261 ccgctgaaga aacagaaaac ctcacactgg aatatcctct ctttgtaaaa cctgtatctg
     1321 aaggtaccgg aaaaggcata actgaaaaaa atctcttaaa ttcgccggca gaactgaaag
     1381 agatggcagg atatcttatt acaagattcg gacaacccgc acttgttgaa gaatatctcc
     1441 ccggcaggga attcactgtc ggtgtcgttg gtacgggcag tgatgccatg gtcatcggag
     1501 gtatggaaat cgagtgtgcc gggaataatc cctattccgt ggaattcaag gaaaattacc
     1561 gtgagtactg tcagtatatt cccatagctg aagagttcag tgacgaatgc agtaaaattg
     1621 cccttgacgc atggaaggca atcggggctg ccgatgcagg aaggattgat ctgaaagctg
     1681 acagaaaggg aagaatatgc tttatggaag taaatccgct tgcaggcctt cacccggtcc
     1741 attccgacct gcctatcctg gcaggcatga gaggtattga ctttcagact ttgattgaaa
     1801 tgattgtaaa atcatccctg aaacgccata atctagtgtg atgaataaaa agagatgttg
     1861 tatcctgtac aaccagcccc ttgaaaatgc attggcagat gagcttg
//
LOCUS       DAOSPX010000445         1874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_56930_length_1874_cov_4.441451, whole genome shotgun sequence.
ACCESSION   DAOSPX010000445 DAOSPX010000000
VERSION     DAOSPX010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56930_length_1874_cov_4.441451"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLZ75_14275"
     CDS             complement(<1..209)
                     /locus_tag="PLZ75_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HOS73609.1"
                     /translation="MGARKRISAERRKEASLARSQAVLKNCPTSPRKMRLATDLIRGM
                     EVNKALDTLKYSSKESSRRLEKLLLS"
     gene            complement(241..504)
                     /gene="rpsS"
                     /locus_tag="PLZ75_14280"
     CDS             complement(241..504)
                     /gene="rpsS"
                     /locus_tag="PLZ75_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOS73610.1"
                     /translation="MSRSIKKGPFIDFKLDKRVLEMNETGKKSVIKTWSRRSVISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFAPTRTFRSHAGKK"
     gene            complement(517..1341)
                     /gene="rplB"
                     /locus_tag="PLZ75_14285"
     CDS             complement(517..1341)
                     /gene="rplB"
                     /locus_tag="PLZ75_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOS73611.1"
                     /translation="MAVRKIKPVTPGQRNKIISAFNTITSTKPEKSLIRPLKRTGGRN
                     VSGKMTMRYMGGGHKRMYRLIDFHRNKDGIHATVRSIEYDPNRSARIALVVYEDGEKR
                     YILAPAGLKTGDRIISGREATPEPGNTMFLSDIPLGTIIHNIELKPGRGAALARSAGT
                     FAQVSAREGKYATVKLPSGETRLVLTTCRATIGAVSNPDHILERSGKAGRSRWKGIRP
                     RTRGVAMNPVDHPMGGGEGKASGGHPRSRKGLWAKGYKTRNPRKYSSKYILERKKK"
     gene            complement(1349..1639)
                     /gene="rplW"
                     /locus_tag="PLZ75_14290"
     CDS             complement(1349..1639)
                     /gene="rplW"
                     /locus_tag="PLZ75_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOS73612.1"
                     /translation="MNILLKPIVTEKMTEQGDKFNRYGFIVAKNANKIQIKKAVEELY
                     GVTVEDVNTINYGGKKKTRNTRSGLLVGRTAATKKAIITLAEGSKIDFYSNI"
     gene            complement(1660..>1874)
                     /gene="rplD"
                     /locus_tag="PLZ75_14295"
     CDS             complement(1660..>1874)
                     /gene="rplD"
                     /locus_tag="PLZ75_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOS73613.1"
                     /translation="EFIKIGDSLNLSNKNSLFVLPEQNKNIYLSSRNVQGVEVITARE
                     INTYKIMKASTLVLVESAVDVLQSAL"
BASE COUNT          394 a          465 c          371 g          644 t
ORIGIN      
        1 gaaagaagca gcttttccag cctgcgtgaa gattcctttg agctgtactt caatgtgtca
       61 agtgccttat tcacttccat gcccctgatg agatcagtag ccagcctcat cttccttggt
      121 gaagtgggac agtttttcag cacagcctgg cttcttgcca gagaggcctc ttttctcctt
      181 tcagctgaaa tcctttttct tgcacccatc ttattatgtt ttcaatgttt actaatgtgt
      241 ttatttcttg cccgcatgag acctgaaagt ccgtgttggg gcaaattccc ccagcttatg
      301 tcccaccatg ttttcagtaa tatacacggg tatgaacttg ttcccgttat gaaccgctat
      361 ggtatgtccg acgaagtcag gggatatcac tgatcttctt gaccaggtct taatcactga
      421 ttttttcccg gtttcattca tttccaggac acgcttgtca agtttaaaat caatgaaagg
      481 acctttttta atcgatctgc tcatagtaca tatatattat ttcttctttc tttccaaaat
      541 atactttgag gaatattttc tggggttcct cgttttgtat cccttcgccc acagtccctt
      601 gcgtgatctg ggatgtcctc cggatgcttt cccttcaccg ccccccatgg gatggtccac
      661 aggattcatt gcaactcccc ttgttctcgg acggattcct ttccagcggg aacggccggc
      721 cttgcctgac ctttcaagaa tgtggtcggg atttgatact gcacctattg ttgccctgca
      781 ggttgtaagt acaagcctgg tttctcccga gggaagctta actgttgcat acttgccttc
      841 acgggcggat acctgtgcaa aggttcccgc gcttcttgcc agggcagctc cccttccggg
      901 cttgagttca atgttatgaa ttatggttcc cagaggtata tcactgagaa acattgtatt
      961 gccgggctcc ggagtagctt ccctgccgga aatgatcctg tcgccggtct tgaggcctgc
     1021 cggagccagg atataacgct tctccccgtc ctcatacaca acaagagcta ttctcgccga
     1081 tctgttggga tcatattcaa ttgacctgac tgtggcatga atgccatcct tgttcctgtg
     1141 aaagtctatc agcctgtaca tcctcttgtg tccgcctccc atgtatctca ttgtcatctt
     1201 tccgctgaca ttccttccgc ctgttctttt aagcggacga ataagggatt tctccggctt
     1261 cgtgctggta atagtattaa aagcactgat aatcttgttt ctctgacctg gtgttacagg
     1321 ttttatcttc ctgactgcca ttttattttt atatattgct gtagaaatct attttgctgc
     1381 cttcggcaag ggtaatgatc gccttttttg ttgctgccgt cctgccaacc agaaggcccg
     1441 acctggtatt ccttgttttt ttcttgcctc cgtagtttat ggtattgaca tcttcaaccg
     1501 taacaccgta aagttcctca acagctttct ttatctgtat cttgtttgca ttctttgcaa
     1561 cgatgaagcc atagcgattg aacttatcac cctgttctgt catcttttcc gttactatcg
     1621 gttttagtaa aatattcatc tcttaaattt tactggtttt tacaaagctg actgcaaaac
     1681 gtcaaccgca ctctccacaa gtaccagggt tgaagccttc attattttgt atgtgtttat
     1741 ttctctggct gttataacct caaccccttg cacatttcgg gatgacaaat atatattttt
     1801 attttgttcc ggtaaaacaa acagtgaatt tttgttgctc agattcaggc tgtcacctat
     1861 tttgatgaat tcct
//
LOCUS       DAOSPX010000446         1804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_60229_length_1804_cov_4.187536, whole genome shotgun sequence.
ACCESSION   DAOSPX010000446 DAOSPX010000000
VERSION     DAOSPX010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60229_length_1804_cov_4.187536"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PLZ75_14300"
     CDS             <1..994
                     /locus_tag="PLZ75_14300"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOS73614.1"
                     /translation="TSDSAVFQFIGTQFSHHRWNGLHFWDLIQPFFMFIVGVAIPLAV
                     SNRLKRGHSQKSITGHAIKRSLLLLLFGTWLAWIGPGKIVFRFQNVLAQLSVTYIIAY
                     FLMKKSYSFQLIFSILLLIIVDLAYRFFPVEGFNHPWVAYENLGAWVNNKIEGVDKSS
                     IWASLNALPTTAHTIWGVLCGKLLMSDKTHGEKIKIMVIAGVSGLVIGYSLDWLGITP
                     IIKKIATMSFVFASGGWAVLALCFSYWLIDVKKKFRKGSMFFIYVGANCIFIYLFCQL
                     GGDSILIRTFNPIFNTFFSWGGEKLVSMLNGTATWITCWYLCYWMYRNRIFIKI"
     gene            complement(1157..>1804)
                     /locus_tag="PLZ75_14305"
     CDS             complement(1157..>1804)
                     /locus_tag="PLZ75_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS73615.1"
                     /translation="KLVNDLGMEILSSHTQVEAEGITLENATKMAEDHARLGVSYCIQ
                     PWVVEEARTTIASYQKMASDWNKVGQIMKDFGIKFGYHNHNFEFDTVEGKIPYFDVFM
                     TELDKDLVTMEIDLFWTTKAGQDPVEIINRYPGRFELFHMKDMFTNEPPFFTTDGVRD
                     FAPVGAGVIDFKRILAAGETAGMKYMIVEQDLSRDNDPFRDIKTSITNLQTKILV"
BASE COUNT          493 a          383 c          378 g          550 t
ORIGIN      
        1 aacatctgat tcagctgttt ttcagtttat cggcacccag ttcagtcatc acaggtggaa
       61 tggcctgcac ttctgggatc ttatacaacc atttttcatg ttcattgtcg gggttgccat
      121 tcctctggca gtcagcaaca gactgaaaag aggacattca caaaaatcaa ttaccggcca
      181 tgcaataaaa agatcattgc tgttattatt gttcggaacc tggttagcat ggataggccc
      241 cggaaaaata gtcttcagat tccagaatgt tcttgcacag ttatcagtta cctatataat
      301 agcgtatttt ctaatgaaaa aaagctattc ttttcaactg attttctcaa tcttgctcct
      361 gataattgta gatttagcct atcgtttttt ccctgttgaa gggtttaatc acccatgggt
      421 tgcctatgaa aatcttggag catgggttaa taataaaata gaaggtgtcg ataaatcaag
      481 tatctgggca agtctgaatg cactcccgac aacagcacat acaatatggg gggtcctgtg
      541 tggcaaatta ctaatgagtg ataaaacaca cggagaaaaa ataaaaatta tggtcatagc
      601 aggagtgagc ggattggtaa ttggctattc cctggattgg ctcggtataa caccgattat
      661 caaaaaaatt gccacaatgt cttttgtttt tgccagcggc ggctgggctg tccttgcact
      721 ttgcttcagt tactggctta ttgatgtgaa gaagaagttc agaaaaggtt ccatgttttt
      781 tatttacgtg ggcgcaaact gtatatttat ttaccttttc tgccagctgg gaggtgacag
      841 catactgata agaaccttta atccgatatt caataccttc ttttcatggg gtggtgaaaa
      901 actggttagc atgctgaacg gtacagctac ctggataacc tgctggtatc tgtgctactg
      961 gatgtacagg aacaggattt ttattaaaat ataaaagaaa aaagtaccgg tacggttgct
     1021 tcaacaagtg cccggtatta caaaataatt gcttaaggct tattggaatt ttccattgca
     1081 agcccggaag aaattttaaa tcaaagccgt cttccgggct ttagaatcaa caatataacg
     1141 gtccgtctgg ctttatttaa accagaatct ttgtctgaag attggtgata ctggtcttga
     1201 tatccctgaa gggatcattg tcacggctca gatcctgttc aacgatcata tatttcattc
     1261 cggcggtttc acctgcggcc agaattcttt tgaaatcgat gacaccggca cctaccgggg
     1321 caaagtcccg tactccgtcg gtcgtgaaaa agggcggttc attggtgaac atgtccttca
     1381 tgtgaaataa ttcaaacctg cccggatacc tgttgatgat ctctaccgga tcctgccccg
     1441 ccttggtcgt ccagaacaga tcaatctcca tggtaaccag gtctttgtcc agttcggtca
     1501 taaaaacatc gaaatagggt atcttgccct ctacagtatc gaattcaaaa ttatgattat
     1561 gatatccgaa tttgatgccg aaatccttca ttatctgacc aaccttgttc cagtcagatg
     1621 ccattttctg ataactcgct atggtagtac gggcctcttc caccacccat ggctggatac
     1681 agtaactgac tccgagcctg gcatgatctt ctgccatctt tgttgcattc tcaagggtaa
     1741 tgccctctgc ttcaacctgg gtatggctgc ttaatatttc cattccgagg tcattgacca
     1801 gctt
//
LOCUS       DAOSPX010000447         1651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_68650_length_1651_cov_3.676065, whole genome shotgun sequence.
ACCESSION   DAOSPX010000447 DAOSPX010000000
VERSION     DAOSPX010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68650_length_1651_cov_3.676065"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLZ75_14310"
     CDS             <1..786
                     /locus_tag="PLZ75_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73616.1"
                     /translation="MGLLYGNGDPMETLEITTRCGQDSDCNPSNALAVLGVMKGFSSL
                     PEGIKEGVKAVGDSTFIHTGYSFNSAVEATYRYALGLISENGGRTGKNKIRIKLQEPT
                     EPPLEIAFPDLVFDRSISVFDEKSWSARGKWETREIRGGDGTRIRQSRYSGRKGNELE
                     LTFNGTGIALYGNWFRDGGRADVYVDGNLHRTVDTYYHYANQQHTECIWHVLNLQPGE
                     HTMKLVVRDEKRSESEGTNIYITEAKIFKTGKKKNESFRFSFE"
     gene            850..>1651
                     /locus_tag="PLZ75_14315"
     CDS             850..>1651
                     /locus_tag="PLZ75_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73617.1"
                     /translation="MKIDYLLKRSSGPILLAAFLLTAVPETDAYSQFVQKQHRPVKKG
                     EIPVSGPGCFDTAGKTYVLVSDISSPQSAVFLGRDVTLDLNGYTISFADAGYGHVPNY
                     GFEEGLEGWDLSRAPGAKLENTEEVHAFIGNRILRLRKGDEIVSGYVYLPVEKRSYYA
                     MCGVTGNYYSDMGGDLSRDMKVSIFVEDEQGNGIECLTEYSDSAMVSCPVISRSPRLG
                     GGFVLAHLNNLPAGRYRIRVRAETDCLVDEIDIRPAMDAGIGIVGRTHP"
BASE COUNT          468 a          369 c          440 g          374 t
ORIGIN      
        1 atgggcctgc tttacggaaa tggcgacccc atggaaaccc tcgaaataac aacccgctgc
       61 ggacaggact cagactgtaa tccttccaat gcccttgcgg tactgggtgt tatgaaggga
      121 ttcagctccc tgcccgaagg tataaaggaa ggtgttaaag ctgtcgggga ttcaactttc
      181 attcacaccg gctattcctt caattccgct gttgaggcaa catacaggta tgcactcggc
      241 cttatcagcg aaaacggagg cagaacaggg aaaaacaaaa tcaggataaa actacaggaa
      301 ccaacggaac ctcccctgga gatcgctttt cccgatcttg ttttcgacag gagcatttca
      361 gtgtttgacg aaaagtcatg gtcagccagg ggcaaatggg aaaccaggga aatcagaggc
      421 ggagacggaa ccagaatcag gcagtccaga tattccggca ggaagggtaa tgaacttgaa
      481 ctcactttta acggaacggg aattgccctt tacggaaact ggttcaggga tggcggcagg
      541 gcagatgtat atgttgacgg caatctccac cggacagttg atacctacta tcattatgca
      601 aaccagcagc atacggagtg tatctggcat gttctgaacc ttcagcccgg agaacatacg
      661 atgaaactgg tggtaaggga tgaaaagcgc agtgaatctg aaggcacaaa catatatatc
      721 actgaggcaa aaatttttaa aaccgggaag aaaaagaatg aatccttccg gttttcattt
      781 gaatagcagc ctgatttacg gggaaaaagc ctttgcggat ccgggtgcat taaaaattat
      841 acaccattta tgaaaattga ttatctgctg aaacggagtt caggaccgat tctccttgcg
      901 gcattccttc tcacggccgt tccggaaaca gatgcttaca gccagtttgt acagaagcag
      961 caccggccgg ttaagaaagg ggaaattccg gtaagcggac ccggctgctt tgataccgca
     1021 ggaaaaacat atgtcctggt cagtgacata tcttcaccac aaagtgcggt tttccttggc
     1081 agggacgtta cccttgacct caatggctat actatctcct ttgcggatgc aggttacggg
     1141 cacgttccca actacggctt tgaagaaggc ctcgagggat gggacctcag cagggctccg
     1201 ggtgcaaagc ttgaaaacac tgaggaggtc catgctttta tcggaaacag gatactgaga
     1261 ctcaggaaag gagatgaaat agtttccgga tatgtttatc tgcccgtgga aaaaagatca
     1321 tactatgcca tgtgtggcgt gacaggaaat tactacagcg acatgggggg tgatctgagc
     1381 agggatatga aagtaagcat atttgtggaa gatgaacagg gaaacgggat tgaatgtctc
     1441 actgaatatt ctgattccgc catggtgagc tgtccggtca tcagccggtc tccccggctt
     1501 gggggaggat tcgtccttgc ccatctgaac aatcttccgg cgggaagata ccgcatccgg
     1561 gtaagggccg aaacagactg cctggttgat gagatcgata tacgccctgc aatggatgcc
     1621 ggtataggca ttgtgggcag aacacatccc c
//
LOCUS       DAOSPX010000448         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_70606_length_1620_cov_5.414058, whole genome shotgun sequence.
ACCESSION   DAOSPX010000448 DAOSPX010000000
VERSION     DAOSPX010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70606_length_1620_cov_5.414058"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PLZ75_14320"
     CDS             <1..1240
                     /locus_tag="PLZ75_14320"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS73618.1"
                     /translation="PKEKRASIINTHWGGVTEDNSFGTHEFMKLTELIDCDAYINGNV
                     GSGTVREMAEWVEYLTSGSDSPMTRLRKENGREEPWKVKYFAIGNENWGCGGNMTAEF
                     YTDVMRQYSTYLNNYGDNRLYRVACGPSDYNYAWTETLMKDPGSRARFQGLSIHYYSL
                     VDGWNKKGSATEFGEREWFRTMSSNLQMDVIIRGHKAIMDRYDPQKTKGLVVDEWGNW
                     FDVEPGTNPGFLYQQNTMRDALTAAIHLNIFNKHADRVHVANLAQTVNVLQSVILTSD
                     DKMVLTPTWHVFRMFRVHQNARLLTTDLKCEEYEFQGSRITALSASASVDNEGKIHVS
                     LANLNPNKEITVTCPVIGAVIKTVRGEILTADQMNACNTFEKPETVKTGQFRGFKVKD
                     GVITVRMPAKSVVMLELEEK"
     gene            1259..>1620
                     /locus_tag="PLZ75_14325"
     CDS             1259..>1620
                     /locus_tag="PLZ75_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOS73619.1"
                     /translation="MKTSLNLRRYLFGILAAGIGFVYGCQENRSGKDYPIRPVPFTSV
                     RLTDNFWAPRIVKNAEVTIPIAFGYCESTGRIKNFEIAGGLDTGKFQTIYPFDDSDVY
                     KIIEGAAYSLQTKPDPKL"
BASE COUNT          477 a          333 c          421 g          389 t
ORIGIN      
        1 accaaaggaa aaaagggcat caatcatcaa tacccactgg ggaggtgtta ccgaagataa
       61 ttctttcgga acccacgagt ttatgaaact taccgagctg attgactgtg atgcatacat
      121 caacgggaat gtaggctccg gtactgtcag ggagatggcg gaatgggtgg agtacctgac
      181 gtccggttca gatagtccca tgacccggct ccggaaagaa aacggaaggg aagaaccctg
      241 gaaggttaaa tattttgcca taggcaatga gaactggggc tgcggcggaa acatgacagc
      301 cgaattttat accgatgtga tgcggcaata ttctacctat ctcaacaatt acggtgataa
      361 caggctatac agggtagcat gcggtccttc ggactataat tatgcctgga ccgagaccct
      421 gatgaaagat ccggggagcc gtgcaagatt tcagggattg tcaatccatt attattcact
      481 tgtggacggc tggaataaaa agggttccgc caccgagttc ggagaacggg aatggttccg
      541 gacaatgagc tcaaatctcc agatggatgt gattataagg ggccacaagg caataatgga
      601 ccgttatgat ccccagaaaa caaaaggact ggtggttgat gaatggggta actggttcga
      661 tgttgagccc ggtacgaacc ccggattcct ttaccagcag aatacaatga gggacgcact
      721 tacggcagcc attcacctga acattttcaa taagcatgcc gaccgtgttc atgtggccaa
      781 tctggcacag acagtaaacg ttcttcagtc ggtcatactt accagtgacg ataaaatggt
      841 tcttaccccg acctggcacg tgttcaggat gttcagggtt catcagaatg caagattgct
      901 tacaacggat ctcaaatgtg aggaatatga atttcaggga agcagaatca ctgccctgtc
      961 ggcttcggct tctgttgata atgaaggaaa gattcatgtt tcactggcaa atctcaatcc
     1021 aaacaaggaa atcacggtga catgtccggt tatcggagcc gttatcaaaa cggtaagggg
     1081 agagattctg actgcagacc agatgaatgc ctgcaataca tttgaaaaac ctgaaacggt
     1141 gaagaccggg cagttcagag gctttaaagt gaaagacggg gtgattacag tcaggatgcc
     1201 ggcaaaatcg gtggtgatgc ttgaactgga ggaaaaataa attcagtgtg acagacgaat
     1261 gaaaaccagt ttaaacttaa gaagatatct tttcggcatt cttgcagccg gaatcggctt
     1321 tgtttacggt tgtcaggaga acagatccgg gaaggattat cccatccggc cggttccttt
     1381 tacttctgta aggctcactg ataatttctg ggctccccgg atagttaaaa atgctgaggt
     1441 aacgatccct attgctttcg gatattgtga atcgacggga aggataaaga atttcgaaat
     1501 tgcgggaggc ctcgatacgg gaaaatttca aaccatttat ccctttgatg attctgatgt
     1561 ttacaaaata attgagggcg ctgcctattc gctgcagaca aaacccgatc cgaagctgga
//
LOCUS       DAOSPX010000449         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_70622_length_1620_cov_2.995527, whole genome shotgun sequence.
ACCESSION   DAOSPX010000449 DAOSPX010000000
VERSION     DAOSPX010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70622_length_1620_cov_2.995527"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PLZ75_14330"
     CDS             <1..958
                     /locus_tag="PLZ75_14330"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOS73620.1"
                     /translation="AEKGVGPLSRPLVFTGHFDTVPPGNMPWKKDPFTGGVYDKRIWG
                     LGTSDMKGALASIMIAAIEAGKVKTLKNGIRLIFTADEEPGCRGALQYARSFHGSQKA
                     GAIIVGEPTSNLPVTAHKGAIYLKAEYRGKTAHSSMPEKGINAVYKAAGAVCRLSQLD
                     FGTEADPLLGYPTLNIGKFTGGRNINSVPDHAEFTIDIRTTSGKDHGKILELVKNTLG
                     KDAAVEKLVDIEPLHTAAEDPFIRLVYDICGVDPGNLDFPLALPYLTDGAVLQQFYNN
                     APTVILGPGEPEQAHRADEHCHLEKLTESVGIYKEIISQWNG"
     gene            966..>1620
                     /locus_tag="PLZ75_14335"
     CDS             966..>1620
                     /locus_tag="PLZ75_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOS73621.1"
                     /translation="MDLKKFTDEEHLDEFLSRPGKEVKEMFSKIDGDIIFLGATGKTG
                     PYLARMAKRACDEAGIRKRIIGVARFSRPGQKEYLEKHGIETICGDLLDTGFLGSLPQ
                     VRNVFYLAGMKFGAVDNLPVTWAVNSWLPGLVAEHFRNSRIAAFSTGCVYPLVPAASG
                     GSVETDPPEANGEYAQSCLGRERMFEYGSNKYKTPVVLIRLNYAVEMRYGVLVDIASK
                     "
BASE COUNT          460 a          358 c          417 g          385 t
ORIGIN      
        1 tgccgagaaa ggagtcggtc cgctgagccg tccgctggta tttacaggtc attttgatac
       61 tgttccgccc ggaaacatgc catggaaaaa agatcctttc acaggcgggg tgtatgataa
      121 aaggatatgg ggactcggaa cctcagatat gaaaggtgca cttgcatcaa tcatgattgc
      181 tgcaattgag gccggaaaag taaaaaccct taaaaacggc atacggctga ttttcactgc
      241 tgatgaagaa cccggctgca ggggtgccct gcagtatgca aggtcctttc acggttcaca
      301 gaaagccggg gcaataattg tcggggaacc aacctcaaac cttcctgtta cggctcataa
      361 aggggcaatt tatctgaaag ctgaatacag ggggaaaaca gcccattcat ccatgcctga
      421 aaaagggata aatgctgttt acaaggccgc aggtgctgtc tgcaggctta gtcagctgga
      481 ctttggaacg gaagcggatc ctctgcttgg gtatccgact ctgaatatcg ggaaattcac
      541 tggaggaaga aatataaatt cagttcccga tcatgcggaa tttaccattg acatcagaac
      601 tacctccgga aaggatcacg gaaagattct ggaactggtg aaaaacacgc ttggcaaaga
      661 tgctgccgtg gaaaaactgg ttgatattga accattgcac acagctgcag aggatccctt
      721 cataaggctt gtttacgata tctgcggcgt tgatcccgga aatctggact tccctctggc
      781 ccttccctat ctgaccgacg gagcagtcct gcagcagttc tacaataatg ccccgacggt
      841 tattctcgga ccgggagagc cggaacaggc acacagggcg gatgaacact gtcatcttga
      901 gaagctgact gaatctgtcg gcatttataa ggaaataata tctcaatgga acggataata
      961 tgaatatgga tttaaaaaaa tttactgacg aggaacatct ggacgaattc ttatccagac
     1021 cgggaaaaga agttaaagag atgttttcaa aaatcgatgg cgacatcatt tttctcgggg
     1081 caaccggaaa aaccggacca tatctggcaa ggatggccaa aagggcctgt gatgaagccg
     1141 ggataagaaa acgcataata ggtgttgccc gctttagccg tccgggacaa aaggaatatc
     1201 ttgaaaaaca tggtattgaa accatctgtg gcgaccttct tgataccgga ttcctgggaa
     1261 gccttcctca ggtcaggaat gtattctacc tggcaggcat gaaattcggg gcagttgaca
     1321 atctgcctgt gacctgggct gtgaattcct ggcttcccgg acttgttgcg gaacatttcc
     1381 gcaattccag aattgcggca ttttccaccg gctgtgtata tccgcttgtt ccggctgctt
     1441 caggcggttc agtggaaaca gatcctcctg aagctaacgg tgaatatgcc cagtcatgcc
     1501 tgggaaggga aagaatgttt gaatacggca gcaacaaata caaaacacct gttgtcctta
     1561 tcagactcaa ctatgctgtt gagatgaggt acggtgtcct ggttgatata gcatcaaagg
//
LOCUS       DAOSPX010000450         1607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_71479_length_1607_cov_3.979381, whole genome shotgun sequence.
ACCESSION   DAOSPX010000450 DAOSPX010000000
VERSION     DAOSPX010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71479_length_1607_cov_3.979381"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLZ75_14340"
     CDS             <1..441
                     /locus_tag="PLZ75_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73622.1"
                     /translation="KGDTKVQKQILSMLSMVGIAVIVTVITAAGQESLLTVGGVLLLV
                     VTIQNLLGYVLGYNAGRLFRLPKRDCRTLAFEVSMSNGGLASGLAMTIGNIATVGLAP
                     AIYGPLMNVTGSTIASYWRTKPIDPNEKHGDRKSKKAVEEKKDN"
     gene            484..948
                     /locus_tag="PLZ75_14345"
     CDS             484..948
                     /locus_tag="PLZ75_14345"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOS73623.1"
                     /translation="MMKKLSIIFSLFALSVLFNLCTTDKPVQAVDEAEQIRNVTAAVE
                     DLKTAIISADRDLLENIASDKLVYGHSGGKVQNKTEFIEEIVSLIPNDYLTIDLADQT
                     IMVSGNTAVVRHIYSSDYLSDGVPGSLRIGNVLVWQLQDGKWKLLARQAYRL"
     gene            1037..>1607
                     /locus_tag="PLZ75_14350"
     CDS             1037..>1607
                     /locus_tag="PLZ75_14350"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOS73624.1"
                     /translation="MARFTRTQVILKMAESGMIPVFYNSDPEVCKQVISACYKGGVRL
                     FEFTNRGAYAHLVFEEINKWAAVEYPDLIMGTGSVIDAPTAILYAQLDSNFIVSPLLN
                     EEMAYALNRRKILWSPGCGSVSEISRAESLGAEVVKIFPGSQVGGPKFVSAVRGPMPW
                     TNIMPTGGVEPTESNLTEWFKAGTFCVGMG"
BASE COUNT          474 a          313 c          404 g          416 t
ORIGIN      
        1 aagggcgaca caaaggttca gaaacaaata ctttcaatgc tttcaatggt tggaattgct
       61 gtaatagtaa cagtaatcac tgcagcagga caggaaagcc ttctcactgt cggcggtgtt
      121 cttctgcttg tcgtcactat acagaatctc cttggatatg ttctgggtta caatgccggg
      181 aggcttttca ggctgcccaa gcgtgactgc agaaccctgg cattcgaggt gagcatgtca
      241 aacggtggac tggcttcggg acttgccatg acaatcggca atattgccac ggtgggactt
      301 gctcctgcca tttacgggcc gcttatgaat gttaccggct caacaattgc aagctactgg
      361 cgtacaaagc cgatcgatcc caatgaaaag catggtgaca gaaaatcaaa gaaagctgtt
      421 gaagaaaaaa aggataattg atcagtatag gaaaaatatt aattatttta atgtaaaaat
      481 ttaatgatga agaaattaag cattatattc agtttgtttg ctttgtctgt cctttttaat
      541 ttatgtacca cagacaaacc ggtacaggca gtggatgagg cagagcagat aaggaatgtg
      601 actgcagccg tggaggatct taaaactgca atcataagtg ccgacaggga cctgctggaa
      661 aatattgctt cagacaagct tgtgtatggc cactccgggg ggaaagttca gaacaagacc
      721 gagttcattg aggaaattgt cagtctgatt cccaatgatt atctcaccat tgacctggca
      781 gatcagacca ttatggtatc ggggaacact gcagtggtaa gacatatcta ttcttcagac
      841 tatctgagtg acggagttcc gggaagcctg aggataggaa atgttctagt ctggcagttg
      901 caggacggta aatggaaact actggcaaga caggcttaca ggctttaata gaggaagtat
      961 atctcattcc ttttctgtat ggatagcgac agaacggaaa aataattaat gatttaaata
     1021 aaacaaggat attaatatgg cacgttttac aagaacacag gtaattttaa aaatggcaga
     1081 atccggaatg atcccggtat tttacaacag tgatcctgaa gtctgcaaac aggttatttc
     1141 agcctgttac aagggagggg tcagattgtt cgaatttaca aacagggggg cttatgctca
     1201 ccttgtattt gaggaaataa acaaatgggc cgcagtagag tatccggatc ttataatggg
     1261 aaccggatcg gtcatagatg ctccgactgc catcctttat gcccagcttg attcaaactt
     1321 catcgtttca ccccttctga acgaggaaat ggcatatgcc ctcaacagac ggaagatact
     1381 ctggtcgccg ggctgcggat cggtttcaga aatatcaagg gcggaatcac ttggtgccga
     1441 ggtagtgaaa atatttccgg gatcgcaggt tggcggaccg aaatttgttt cagcagtaag
     1501 gggacccatg ccctggacaa atattatgcc caccggtggc gttgaaccca cggaatccaa
     1561 tctgacggaa tggtttaagg caggtacttt ctgcgtgggg atgggtt
//
LOCUS       DAOSPX010000451         1601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_71892_length_1601_cov_3.813713, whole genome shotgun sequence.
ACCESSION   DAOSPX010000451 DAOSPX010000000
VERSION     DAOSPX010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71892_length_1601_cov_3.813713"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLZ75_14355"
     CDS             complement(<1..624)
                     /locus_tag="PLZ75_14355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS73625.1"
                     /translation="MERVMSELASNFAERSDLKVHIVLYGITRELFYPLRGEIIIHTP
                     AFRFNNSLRVFSTMRTMLYLRTEIRKISPDVILSFGEYWNSFVLITLKLLPYRIYISD
                     RCQPDKVLGKLHDWLRKKLYPGASGIIVQTKKAEEIYRSEYKNRNIHTIGNPVRSIAT
                     RSNVQRENIILTVGRLIKSKNHDKLIEIFIRISKPDWKLVIVGYDHLK"
     gene            complement(757..>1601)
                     /locus_tag="PLZ75_14360"
     CDS             complement(757..>1601)
                     /locus_tag="PLZ75_14360"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOS73626.1"
                     /translation="AIYVDAVYNNASHHNGITFLLATVFFSFQIYCDFSGYSDMAVGA
                     ARVMGFKLTNNFNLPYLSKSVAEFWSKWHISLSNWLRDYIYLPMVSTLSKKITKKKVM
                     GIDRPVWLYSGATLVTFIVSGIWHGAAWTFVMLGLVHAFFLIFAIVTKSFRLNMATKT
                     GITKVPFLYDFLQIIITFSLVTFAYIFFRANSFSDVSLIIKSFVHMKGPVFYENYKDI
                     AYPVIGILFLTFVELKRRYFNESFAVMNNRKWYVQSAACATLIIVILLVGVFDGSQFI
                     YFQF"
BASE COUNT          482 a          324 c          331 g          464 t
ORIGIN      
        1 tttaagatgg tcatatccaa caataaccag cttccagtca ggtttggaaa tcctgatgaa
       61 tatctcaatc agcttgtcat gattctttga tttaatcagg cgtcccactg tgagtattat
      121 attctcccgc tgtacattgc tcctggtggc aatagaccgc acaggatttc caatagtgtg
      181 aatatttctg ttcttatatt cagatctata tatttcctct gcttttttgg tctgcacaat
      241 gattcctgaa gcccccgggt atagcttctt gcgcagccag tcatgcaatt ttccgagaac
      301 tttgtccggc tggcatctgt ccgaaatata aatgcgataa ggaagtagtt taagagtaat
      361 caggacaaaa ctgttccagt actctccgaa actcaggatt acatcaggcg aaatctttct
      421 gatttctgtt ctcaggtaaa gcatcgtccg cattgtactg aaaaccctga ggctattatt
      481 gaacctgaag gccggtgtat gaataattat ctctccccga aggggataaa ataattcccg
      541 cgtaattcca tatagtacaa tatgcacttt aagatcagat ctttcggcga aatttgaagc
      601 cagctcagac atcactcgtt ccattcctcc tgcctgcagg gaggggataa caagacatag
      661 ttttttcaga tgcattatat tgcaaagatt aagcaggctg atcagtttct gtcagtcttc
      721 ttttgtttag taacactgag aaaacaggtt gggagattaa aactggaagt aaataaactg
      781 acttccgtca aaaacaccta ctagaagaat aacaatgata agggtagcgc aggctgctga
      841 ctggacatac cattttctgt tattcattac agcaaaagat tcgttgaagt atcttctttt
      901 cagttcaaca aatgtcagaa acagtatacc gattaccggg tatgctatgt ctttatagtt
      961 ttcgtagaac accggacctt tcatatgcac aaagctcttt attatcaaag atacatcact
     1021 gaagctgttt gcccggaaaa agatatatgc aaatgtaacg agtgaaaaag taatgattat
     1081 ctggaggaag tcatagagga acgggacttt ggtaatgcct gttttcgtgg ccatattcaa
     1141 ccggaaggat tttgtaacga tagcgaatat caggaaaaaa gcatgcacca atcccaacat
     1201 aacaaaggtc catgcagccc cgtgccatat gccgcttaca atgaaggtaa ccagggtagc
     1261 tcctgaatag agccataccg gcctgtcaat tcccatcact ttcttttttg taatcttttt
     1321 tgacagtgtg gataccatag gcaggtatat ataatcacga agccagttag agaggctgat
     1381 gtgccattta ctccagaatt ctgcgactga tttggaaaga tatggcagat tgaagttatt
     1441 ggtaagtttg aaacccatta ctctggcggc ccccacagcc atatctgagt aacctgaaaa
     1501 atcacaataa atctggaatg aaaagaaaac ggttgccagg aggaaagtga tgccgttgtg
     1561 gtgagatgcg ttattgtaga ctgcatctac atagatggca a
//
LOCUS       DAOSPX010000452         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_73467_length_1578_cov_2.757715, whole genome shotgun sequence.
ACCESSION   DAOSPX010000452 DAOSPX010000000
VERSION     DAOSPX010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73467_length_1578_cov_2.757715"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="PLZ75_14365"
     CDS             complement(<1..1466)
                     /locus_tag="PLZ75_14365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOS73627.1"
                     /translation="MNDFLIILMLLLPLSVFSQKTTIKGTVTSESGNPLAGATVQVKE
                     TASGTITGPDGKYSIEVDDRSAILVFSFVGYESREIPVQDRNVVNVVLESEIKGLDEI
                     IVIGYGSQKKSDITGTVASLPGNRLEMVPNLNLIQAIMGAVPGIIIETGSAGANPSQS
                     IMIRGRASISADNKPLVIVDGIPYNGSISDINPEDIHKVEILRDASAAAIYGSRGANG
                     VILVSTNEGVAGKTKFSYNGRSGFSRATKLHRLLTGPEFYEFKKTRNPDQISVTEIQN
                     YNDGVSTDWVKLGIRQGRSMEHNLSVSGGFRDTKFYIGGGLTSVEGILKGDDFRRFTS
                     RLNIETGILSWLSAGTRTQLNFDDSGGAVVDFYNTMRLNPLARPFDDEGNYLIYPVPE
                     NIVVTNPLTSLLYDNLDKSYQILTNNYIVVDIPFIKGLSYKLNTGVRISFSDYANYAG
                     TNTYLGYRFMGMSSTANTAGSSGTVENIITFNRQFGKHT"
BASE COUNT          400 a          366 c          331 g          481 t
ORIGIN      
        1 gtgtgcttac cgaactgtct gttgaaggtg attatgtttt caactgttcc gctgcttccg
       61 gccgtgttgg cggtactgga cattcccatg aatctgtatc cgaggtaggt attggttccg
      121 gcgtaattgg cataatcgga aaaactgatt cgcacaccgg tattcagctt gtagctcaga
      181 cctttaatga agggaatatc aacaactatg taattatttg tcagaatctg gtaagatttg
      241 tcaagattat cgtacaacag actggtaaga ggattggtta caacaatatt ttccggaaca
      301 ggatatatca gataattccc ttcatcatca aagggcctgg caagcggatt cagcctcatt
      361 gtgttataga aatccacgac ggctccgcct gagtcgtcaa aattcagctg ggtgcgggtt
      421 cctgccgata accaggataa aataccggtt tcaatattga gtctgctcgt aaaacgccgg
      481 aaatcatcac ctttgagaat gccttcaaca cttgtcagac ctcctcctat ataaaacttt
      541 gtatccctga aacctcctga tactgacaga ttgtgttcca tggaacggcc ctggcggatg
      601 ccaagtttta cccagtcggt tgatacaccg tcattgtaat tctgtatttc agtaaccgaa
      661 atctggtcag gattcctcgt ttttttgaat tcatagaatt caggaccggt cagaaggcga
      721 tggagttttg tggccctgct gaatcctgat ctcccgttgt atgagaattt tgttttgccg
      781 gcgacgccct cattcgtgct tacaagaata actccgtttg ctccccttga tccgtaaatt
      841 gcagctgccg aagcatccct gagaatttcc accttatgaa tatcttccgg attgatatcc
      901 gatatgcttc cgttataggg aataccatca acaatcacca ggggtttgtt gtctgcagaa
      961 atggaagccc ttccgcggat cattattgat tgcgacggat tggcacctgc agacccggtc
     1021 tcgattatta ttcccggaac agctcccatt attgcctgga taagattaag gttcggaacc
     1081 atttcgagcc tgttccccgg cagtgaagca actgttccgg tgatatcact cttcttctga
     1141 ctgccatagc ctatgacaat tatttcatcc agacctttga tttccgattc aagcaccaca
     1201 tttaccacat tcctgtcctg taccggaatt tcacggcttt catacccgac aaatgaaaat
     1261 acgagaattg ccgaacgatc atccacttct attgaatatt ttccgtcagg accggtaatg
     1321 gttcccgatg cggtttcctt tacctgaaca gtggctccgg caaggggatt cccgctttcg
     1381 cttgtgactg tacccttaat ggttgttttt tgcgaaaaca ctgatagtgg aagtaacagc
     1441 atcaggataa tcaaaaagtc attcatcagt ctgttgtttg ggttgaaaat ttccatttca
     1501 aagaaatgaa aatattattt aactgagtat tttttctgat tcatttaagc tgctgcccga
     1561 ctcgttttta tcactcac
//
LOCUS       DAOSPX010000453         1558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_74827_length_1558_cov_3.893546, whole genome shotgun sequence.
ACCESSION   DAOSPX010000453 DAOSPX010000000
VERSION     DAOSPX010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74827_length_1558_cov_3.893546"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLZ75_14370"
     CDS             <1..937
                     /locus_tag="PLZ75_14370"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOS73628.1"
                     /translation="EKVYNTGTGTDILIWEMAEQLRDRIRSGLKEPLFISACPAWNKY
                     AEQFLPSYLPMLSSLKCPQQIAGSLVRTAIADQAGVKPEKIFSVSVTPCTAMKFEARR
                     DGMMRRGISDIDSVLTVRELGRLIRLYGIDLNNSGSEQADSPMEGFSSAASLAELSGG
                     LAEGIIRTLCYSETRQSPARQYMKKLRAGASFRETVIPAGRYEVSVAIVDGLTGLEKL
                     QSSLDAGNKYDLVEVMVCPGGCINGAGMPFRNQKDDRKNLARMIYQADEHDAIDLPEN
                     SPVMMNFYDKLIAENNEIADKKIFHTHYVKRNVLL"
     gene            934..1113
                     /locus_tag="PLZ75_14375"
     CDS             934..1113
                     /locus_tag="PLZ75_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS73629.1"
                     /translation="MKGCKCLIRNVSDDTGFRSEDRQRAAGPEMTGTGTGNRILQGAK
                     GFLRLEQGIQNQEMQ"
     gene            1135..>1558
                     /locus_tag="PLZ75_14380"
     CDS             1135..>1558
                     /locus_tag="PLZ75_14380"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS73630.1"
                     /translation="MLVYTIKELCRTCYTCVRECPARAIRIENGQAEVIEDRCIACGN
                     CTKVCSQGAKVYLNTTGRVSKLLSGNDSVAAIVAPSFPAEFSEIGDYRILVGMLRRLG
                     FRYVAEVSFGADLVAQKYRELVDEGRETFITSDCPSIVS"
BASE COUNT          440 a          346 c          432 g          340 t
ORIGIN      
        1 tgaaaaagtc tataatacag gaaccggaac cgatatcctg atatgggaaa tggcggaaca
       61 gctcagggac aggatcaggt cggggcttaa ggaaccactc tttatctcgg catgtccggc
      121 atggaataaa tatgcagagc agtttctgcc ctcgtatctt cccatgttgt caagcctgaa
      181 atgcccccag cagattgccg gctcgcttgt aaggacagcg attgctgatc aggccggtgt
      241 aaaacccgaa aagatttttt cagtatcggt cacaccctgt actgcaatga aattcgaggc
      301 aaggcgtgac ggaatgatgc gcagggggat aagcgacatt gattcagtgc tgacggtaag
      361 ggaactgggc aggctgatca ggctgtacgg aatagatctg aacaattcag gttcagaaca
      421 ggctgattcc cccatggaag gattcagctc tgccgcatcc ctggcagaat tatccggcgg
      481 acttgctgaa ggaataataa gaaccctgtg ttattccgaa accaggcagt cacccgccag
      541 gcaatatatg aagaaactca gggccggggc ctctttcagg gaaaccgtta tacccgcagg
      601 ccggtatgag gtcagcgttg caatcgttga cgggctcacc ggtctggaaa aactgcagtc
      661 atcgctggat gccggcaata aatatgatct tgtggaagtg atggtttgtc cgggaggatg
      721 catcaacggt gcaggaatgc cgttcaggaa ccagaaagac gacaggaaga accttgcccg
      781 gatgatttat caggctgatg agcatgatgc aatagatctg ccggaaaatt cgccggtaat
      841 gatgaatttt tacgacaaac tgattgctga aaacaacgag atagctgata agaaaatatt
      901 tcacacccat tatgtgaaaa gaaatgtcct gctgtgaaag gctgcaagtg cctgatccgg
      961 aatgtttccg atgacacggg cttcaggtct gaagaccggc agcgggctgc cggaccggag
     1021 atgaccggta ccgggacggg aaaccggata ctgcagggag caaaaggctt tctgagactg
     1081 gaacagggaa ttcagaatca ggaaatgcag taacagaaat aaacacaata aacaatgctt
     1141 gtctatacca taaaagaact ttgccgcacc tgttacactt gtgtgaggga atgtcctgca
     1201 agggctatac gcatagaaaa cggacaggct gaagttattg aagacaggtg tattgcctgc
     1261 ggcaactgca caaaggtgtg cagccagggt gcaaaggtat atctcaacac caccggcagg
     1321 gtatccaagc tcctttcggg aaatgacagt gttgccgcca ttgtcgcacc cagttttccg
     1381 gcggaatttt cagaaatcgg cgattacagg attctggtcg gcatgctccg caggctgggt
     1441 ttcagatatg ttgccgaagt atcattcgga gccgatctgg ttgcgcagaa atacagggaa
     1501 ctggtggatg agggcaggga aaccttcata acatccgatt gtccctccat agtcagct
//
LOCUS       DAOSPX010000454         1525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2308
            NODE_77284_length_1525_cov_4.934014, whole genome shotgun sequence.
ACCESSION   DAOSPX010000454 DAOSPX010000000
VERSION     DAOSPX010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864375
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:39:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,877
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 0 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77284_length_1525_cov_4.934014"
                     /isolate="AMR_MDS_2308"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1525
                     /locus_tag="PLZ75_14385"
     CDS             <1..>1525
                     /locus_tag="PLZ75_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOS73631.1"
                     /translation="RKLEKAFPGLVSLSSAGKSYQGRDIYVLTISDKKSGNPDHKPGF
                     WIDGNIHSIELQGSEMAMYTAWYLCEMFDQNDFINQLLKDKTFYIAPSINPDGRDYFA
                     NIGVPPRSGLIPFDNDRDGLFDEDGPDDLNGDKNISQMRRRNPDGAYKADPKDPRRMI
                     RVEPGEKGEYDLLGMEGIDNDGDGQINEDGPGGYDGNRDWGFNWEPNYVQSGAHKYPF
                     SQPENRAVRDFVSSHRNITAAQSFHNTGGMILRGPSIQNGGAEAYSRSDDAAIEAIGK
                     KGEKILPGYSLLTIWKDLYSVYGGEIDWFHGAMGCFVYSNELWNSYMMFYDRENTDSY
                     EFDKLLLFEDGFIPWQKVNHPVYGEVEVGGFSKMFGRLHPGFMLETDAHRNAAFCLYN
                     AYETPKLEISNLKVTKTEGGLREITASITNTRMIPTHSASNLKFKIDPPVYAFLDGGT
                     VVAGMIVENADLNITSEQKRNPQRIEIPNIAGYQKVDVKWIVKGGSKYTVRVESVKGG
                     "
BASE COUNT          469 a          330 c          395 g          331 t
ORIGIN      
        1 caggaaactt gaaaaagctt tcccgggcct ggtcagcctg agctcagcag gtaaatcata
       61 ccagggacgc gatatatatg tactgacaat atccgataaa aagagcggaa atccggatca
      121 taagccggga ttctggattg acggcaatat ccattcaatt gaactccagg gaagtgaaat
      181 ggccatgtat acagcatggt acctctgcga aatgttcgac cagaatgatt tcataaacca
      241 gttgctgaaa gacaagacat tctatatagc accaagtatc aacccggatg gcagggatta
      301 ttttgcaaat atcggcgttc ctcccagatc aggacttatc ccctttgaca atgaccggga
      361 tggcctgttt gatgaagacg gaccggatga tctgaacggt gacaagaata ttagccagat
      421 gagacgcagg aatcccgatg gtgcatacaa ggccgatccc aaggatccgc gcaggatgat
      481 acgcgttgaa ccgggcgaaa agggcgagta tgatcttctt ggaatggagg gtattgacaa
      541 tgatggagac ggacagatca atgaagacgg acccggggga tatgacggca accgcgactg
      601 gggcttcaac tgggagccaa attacgttca gagcggggcc cataagtatc ctttctcaca
      661 gccggaaaac cgggcggtaa gggattttgt ctccagccac aggaatataa cggctgcaca
      721 gtcattccac aatacaggag gcatgatcct caggggacct tcaatacaga acggcggtgc
      781 tgaagcctac agccggtccg atgatgccgc catcgaagcc ataggaaaga aaggggaaaa
      841 gatcctcccc ggatattcac ttcttacaat ctggaaagac ctgtattccg tatatggcgg
      901 tgaaattgac tggtttcacg gggcaatggg atgttttgtc tattcaaacg aactgtggaa
      961 cagctatatg atgttttatg acagggaaaa caccgattcc tatgaattcg acaaactgct
     1021 tttgtttgaa gacggattca ttccctggca gaaagtgaat catcctgttt acggagaagt
     1081 ggaagtaggc gggttcagca agatgttcgg acggctgcac cccggattca tgcttgaaac
     1141 ggatgcacac cggaatgctg cattctgcct ttataacgca tatgaaacac ccaaactgga
     1201 aatctcgaac ctgaaagtaa caaagactga aggcggtctc agggaaataa cagcctcaat
     1261 aaccaacacc cggatgatac caacgcattc agcatcaaac ctgaaattta aaattgatcc
     1321 cccggtttat gcctttcttg acggaggaac ggttgttgcg ggcatgatag tggaaaatgc
     1381 cgaccttaat attaccagcg aacagaaaag gaatcctcag aggatagaaa tacccaatat
     1441 cgcaggatac cagaaagttg atgtgaaatg gatagttaaa ggcggcagta aatacacggt
     1501 aagggttgaa agtgtcaagg gcgga
//
